LOCUS       DAOPKU010000001        19052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_3043_length_19052_cov_5.083013, whole genome shotgun sequence.
ACCESSION   DAOPKU010000001 DAOPKU010000000
VERSION     DAOPKU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 19052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19052
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3043_length_19052_cov_5.083013"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2248)
                     /gene="fdhF"
                     /locus_tag="PK849_00005"
     CDS             complement(<1..2248)
                     /gene="fdhF"
                     /locus_tag="PK849_00005"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1169..1171),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPE64530.1"
                     /translation="MASIKAIINGKSITAESGMTILQAARANGIHIPTLCDHPALSPF
                     GACRICLVEAEKNPKLLTSCTTPITEGMIVHTNTPRVIEARKAVLELILVRHPLECFS
                     CASNGKCELQDVAYELGVKDSRFADPGDTNTSHKLEDANPFYVRDLNKCILCGRCVRV
                     CEEHAIYHAIDFQGRGIHTSIQPPVGSPIEESDCVFCGQCVQVCPVGALFEKTAAGQG
                     RPWDLEKVKTVCSYCGVGCELEVNVNKKTGKIANVTTNYSSPTALNKGRSCVKGRFAW
                     QFVHSEDRLTTPLIREGDTFRKASWDEALSLVAEKLGEVKTKHGSDALGFFSSARCTN
                     EENYLMQRLAREVMGTNNVDHCAHLUHSATVTGLGETLGSGAMTNDFESIKSADTILI
                     IGSNTTENHPVIGSFIKERKRKGNCTLIVCDPRKIEMAKYADVYIRQKSGSDVVLLNG
                     LMHVILRENLYASDFIEKNVNNFEKLKETMSKYTPQYVSEITGVSPELIEDAARAYAK
                     GPNSAIFYTMGITQHLNGTDNVRTVANLALLCGMLGRPGTGVNPLRGQNNVQGACDTG
                     CLPEVIPGYQKVTLPATQQKVKELWGSDLPATPGLSVVEMMHAAGEGKIKGMYIMGEN
                     SMVTEPDSDHVRHALKKLDFLVVQDIFLTETAEFAHVVLPAASWGEKDGTFTNTCRAV
                     QRVRKAVDSPGESRPDWEILLAVGKRLGASWNDMNSPETIFNAIGRIAPAYAGITYAR
                     LEGGALPWP"
     gene            complement(2263..4056)
                     /gene="nuoF"
                     /locus_tag="PK849_00010"
     CDS             complement(2263..4056)
                     /gene="nuoF"
                     /locus_tag="PK849_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPE64531.1"
                     /translation="MALYRAHVLICHGTGCASGGAPKVLEAFKEELAKKGLEKEILVI
                     ATGCHGMCEFGPIVVVYPEGTFYSQVKPSDVAEIVEEHLYKGRIVTRLLYKEPLSAQK
                     VCHYKDIPFYGKQHRIVLSNCGYINPDNIDEYISRDGYQALGKCLVEMTPAAVIDEMK
                     KSGLRGRGGGGFPTGMKWGFCAASPGDKKYVICNADEGDPGAFMDRSILEGDPHAVIE
                     GMMIGGYAMGADEGYIYCRAEYPLAINRLQTAIKNATEYGLLGEDILGTGFNFTLHIK
                     EGAGAFVCGEETALMASIEGQRGMPRARPPFPAVKGLWGKPTNINNVETWANVPRIIV
                     QGAECFAAMGTEKSKGTKVFALTGKVKNTGLVEVPMGITIREIVFEIGGGIIGDKKFK
                     AVQIGGPSGGCLVDEHLDLAVDYESLTAAGAIMGSGGLVVLDEGNCMVDTAKFFLEFT
                     QRESCGKCVPCREGTKKMLDILTRITEGKGVPEDIDRLLYLGNQIKDASLCALGGTAP
                     NPVLTTLKYFRHEYEAHIFDKKCPAGVCQSLLRYSINTEKCIGCTKCARNCPVSCISG
                     KVKEPHVIDQDKCIKCGACMDNCPVGAVVKG"
     gene            complement(4075..4461)
                     /locus_tag="PK849_00015"
     CDS             complement(4075..4461)
                     /locus_tag="PK849_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPE64532.1"
                     /translation="MTEEKKKIRSLDDLRKIKEKTGDLTSARSEVDKVKVIIGMGTCG
                     IAAGAREIMNAVLKELEVRNLHNVAVETTGCIGMCQNEPLLDVIRPGEPRITYCAVTP
                     PDVSRIVSEHIVNGRIVQDKLYGRAD"
     gene            complement(4499..5041)
                     /locus_tag="PK849_00020"
     CDS             complement(4499..5041)
                     /locus_tag="PK849_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE64533.1"
                     /translation="MLEDLSHHILDIAENGVNAGAESIGVTFDDDGEFIRLTVRDNGR
                     GMSEATARAVVDPFYTTRTERRVGLGLPFLKQLSELCGGEFLLSSAPGKGTTVSASFS
                     KQCVDTPPPGDIPSTLLTLLVGHGHISWKYVHRVGKREFLLDSDELKDALGGGNPFTD
                     PALAVGVKEYIAENIGELYM"
     gene            complement(5044..5523)
                     /gene="nuoE"
                     /locus_tag="PK849_00025"
     CDS             complement(5044..5523)
                     /gene="nuoE"
                     /locus_tag="PK849_00025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPE64534.1"
                     /translation="MVVETKQDVRSMVQEITAPWKGKHGGVIPVLQETQKKVGYLSRE
                     AMEVVSEELGVPLAELYGVATFYAQFHLKPRGRHIIRVCRGTACHVRGSAKIMDKIMS
                     MLKVKENGTTDDLRFTLEPVACLGCCGLAPVVMIDEDTHGRLTDDEAALQEFLDQYA"
     gene            complement(5788..6717)
                     /locus_tag="PK849_00030"
     CDS             complement(5788..6717)
                     /locus_tag="PK849_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64535.1"
                     /translation="MNTTQKIERLLDEAYTTDDPEEMERLARQILNYDKDNVEAIILL
                     ADVTEDSKEKIVLLQKASCLLEKDLEESPVPEGTAFLDDELGMLYAAVLQRLGFSYFS
                     EGENEKALEIAHSIMAFDPEQQTLGRTLYYRILVDMNEYTAILEECLKEREPFPAMLH
                     SKAIAAFRLSGATMPAYRALWEAFAADPDVPFHILGYYEEPDEEAEEEELESYSLAML
                     FEDAWLTEGDAALANWVAGGTILLGLAASLFPAENSEKMLVLADALGLADAAEDIMVK
                     LESRQDWNVLSKQEKVKEALKLLSEGKYLPLKS"
     gene            7016..7555
                     /locus_tag="PK849_00035"
     CDS             7016..7555
                     /locus_tag="PK849_00035"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HPE64536.1"
                     /translation="MGELIAQARGVAIFPSVVKAGFVFGGQYGEGLVLRRDPVKKRWY
                     GPSFVSVTGASWGLQIGAQSIALVLVITNDRGLEGFMGNNVKLGGDLSVAAGPVGRRG
                     EMGTDYKMKASIYSYSMTKGLFAGVSLEGAAINVDRNANQAYWGKPFSAGNALNSAAT
                     KNEIKQLVIELEKIMKRAK"
     gene            complement(7612..8055)
                     /locus_tag="PK849_00040"
     CDS             complement(7612..8055)
                     /locus_tag="PK849_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64537.1"
                     /translation="MAMPMQVSLDEILSMLLARVDSLAINDENSKSKFNIVARVLYKK
                     GILTDADIVESIKEEHRMLKELGAIAEEPSDDIVETIADGILQWIKGDVESLKKGMEE
                     YERKVQEAMAKQQAQKPKIDVASPAVLQQLNRMGGKGQGGGKLII"
     gene            complement(8104..9195)
                     /gene="ychF"
                     /locus_tag="PK849_00045"
     CDS             complement(8104..9195)
                     /gene="ychF"
                     /locus_tag="PK849_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPE64538.1"
                     /translation="MLKCGIIGLPLSGKSTVFNVVTRAGAEVKPYASGKTDPNRAMVN
                     VPDDRFDHLARLFEPKKVTPATVEFVDLAGLSRNASKGEGVGNAFLSFVSESDALVHV
                     VRLFENPDVPHPENSIDPVRDWNIVEMELIFRDLGVIENRISRLQAKKKNIPSETEEK
                     ALLERCQACLLEERPLRELGLSPDELRLLSGFAFVTLKPELIVANLDETQTDDSLPLL
                     NALRELAKTKGIRLLRIFGKMEMELTELPEEEQKEFMGELGIEESGRNRLISEAYALL
                     GLLSFFTTGKDEVRAWTLVKGANAVDAAGAIHSDLARGFIRAQVVSYEDFISHEQSLA
                     KCREKGVLRLEGKDYVVNDGDMIEIRFNV"
     gene            9370..10896
                     /locus_tag="PK849_00050"
     CDS             9370..10896
                     /locus_tag="PK849_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPE64539.1"
                     /translation="MKMGVFNVSLLLFFLLCVLSVPSEAVSEYSILRTDMYPAGARLV
                     FSIPASEKVHFQLPGAFDSASVRPLPSEGVEVLSFEAVDVSRSEWIPPVLEKLFTSIR
                     EKERLQATLGGKISAIENSLALLSGPLPKELKGADVAEYIESVRAVREKMELERASLA
                     DTLDELQKELAVLQADFKSKMPPDSQKAVEITAVLSGSGVFLVEAFTRYAEWVPFYRM
                     NLSVSSGGISAGLFARARQKTGLLVEGTVHFHTSMPSVSVAPPELRPLVADLAPKHKD
                     VRRFAMDEALPMMAPMVQAPAPAEPAPRPVIQTLTDLSTQAEGLLTGDYTPADFNLGS
                     FSLKSETSIISVPSLSEQAWLVAESKGLPFPILPASAELSVDGSPSGKTTLDEHGSGS
                     DILIAFGRTPRVKASKEKLVSKEGSTWTGRGRTEDGYTIEVTNGMPHPVAVTLKDRIP
                     ISSRENISVEVLRIEPKPDEQSEQNLLTWKLSLAPGEKRTINAVFRLSYPSDETVIFR
                     "
     gene            11069..12094
                     /locus_tag="PK849_00055"
     CDS             11069..12094
                     /locus_tag="PK849_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPE64540.1"
                     /translation="MLLLLLYFAFSMFYVFSIKNSLFSQLPPLQMAAQGIIICIVSME
                     AGAHPILPWVGVLAAVSGFFILLHSYSQYGRGTHLPPPVAHAESPSSDTASEPWIRSP
                     FPYVILDNEGTIIDANDAFASFAENGSVQGENVTTFFVPGETEAVVGGKKHAIFQKAK
                     DGQFHFLLVDNPPAQKNSKTAEAKSELELFDPKYGLYSRKYAQLRIPEELGRANRYRR
                     WLCGILLKVNYTYLPGRGRQPALEETFLAAYVLYVKESIRESDMGFSMQDGEFLLLLP
                     ETPQQGAKDTALKLIVVPESLDEMKKDYPFSVDIEYGFLYYSGNYPLSYEQFIQKLYA
                     SLGGSAE"
     gene            12091..13776
                     /locus_tag="PK849_00060"
     CDS             12091..13776
                     /locus_tag="PK849_00060"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HPE64541.1"
                     /translation="MITSKKAVCTLLLGLFFLIPGFMRISEANTRDLSSMTLEEKVGQ
                     IMLVFFEGGAMSENLQKFISEIKVGGVILYSSRNNIESAEQVANLNESIQRRALASSS
                     RPLFIAIDQEGGLIARIREGVTPFPGNMALGAAGDPSLAGRVASVMGEELASLGINMN
                     FAPVVDVNNNPLNPIIGVRSFGSDPQEVARLGAAMISSFLEKGVFPSAKHFPGHGDTE
                     VDSHSGLPIINVSKDRLNSVEFPPFKAAVNAGVPMVMTAHVLVPALDEKNPATLSPAI
                     LGMLRTELNFKGLIITDSMGMAALKKERTMEEAAIEAMNAGADILLFGADKGHEETEH
                     FNMYNALLKACVEGEIPISRLDDAVRKVLEAKEALGLLQDEWEPHFAPRIAPDGAATA
                     REAALRSITVARNRSETLQTVRDGKKRPLLWPGERIAAGKVFCEFLFDVDLIEVPERP
                     ESHDISGILQRVEGVDRILVAEYDSKRNPDWLNLVRQIGEERTLLISLRTPYALLSLP
                     SVSGAIVSYSDNTPSLQALADVLRGNAPAEGRLPVELPGFEIRTGGCRVTSGF"
     gene            complement(14085..14924)
                     /locus_tag="PK849_00065"
     CDS             complement(14085..14924)
                     /locus_tag="PK849_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPE64542.1"
                     /translation="MEDVARFAGVNKATVSRALKGDPRISSVTSEKVWNAAKTLGYRI
                     DRTASGLSGGKTGLAAVVLESLRPWFSPLFFSGLNRVLGNAGMDILLKMPGFSDDALE
                     ASLVSRRVDCILLAGSGEGMAPSRFLLPQIPVVTAGFSIAETPSVFVSEQETLSRLRS
                     VSSGRPLRFVSGTSPLFPFLSRSITDSEAAPEEYFWIMDGCAPEDVGRTVRFGCVCTL
                     PEEPVRTGFFRIEWPAFEMGVAVGRLLMKASSGKDVFPTRLMPVPLVRDPAGEKILFG
                     KMN"
     gene            complement(14956..16602)
                     /locus_tag="PK849_00070"
     CDS             complement(14956..16602)
                     /locus_tag="PK849_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPE64543.1"
                     /translation="MAFTIEVANGPVFSSDVPVTGKSVLSESRPDGEEKTIVAWRVNN
                     YLRSLDWVIEDNARVEFIDTSSFEGMEVYRRSLSFLLVLACKRGLAKDVVLRHSISEG
                     YYWEFVEGEVTDEDLAVIKATMADLVGKDLPFIRKVVPLDKARRIFDRQGSGEVARLF
                     QWGAVDPVELYRCADMYGYYYAPLAPSTGYLKLYDLKKLGPGMALQFPTISYPSSLPP
                     FRASKNLSGVFLDYAKWLEVLDLRTMDSLHKKVAEGRSQEVILIAEAFHAQKLGRIAD
                     EIASKTKVKVAAIAGPSGSGKTTFSERLKIQLLVCGRNPVTLAMDNYFVNREDTPRDE
                     QGNLDFEVVEALELELLEDHLRRLLAGEEVVTPQFSFIEGKKKPGKKIKLGPRDILIM
                     EGIHGLNDRVTGAIAKDELYGIFVSPLTGVNFDNHNRTSTTDNRLLRRLVRDARTRGH
                     SAESTLTLWPKVIRGSMQFVFPYQKHADVMFNSSLPYELAALRAYVDPILHTVPENSP
                     VYGEAQRILSMIKFVPMIPTEIIPNNSIIREFIGGSCFDV"
     gene            16696..17406
                     /locus_tag="PK849_00075"
     CDS             16696..17406
                     /locus_tag="PK849_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPE64544.1"
                     /translation="MENVKGKTKIYDQVVDSIKLRISEGELPQGSALPPERQLIDELN
                     VSRSSLREGFRILEMMGLIESIPGKGRFVRTSRSDGGATGRVPLQDEAILELVEARLV
                     LEPAIAVEAAKHASPSDLTRIRRILTKTGKDVESLEHRAQCDFDFHLVLAESTHNFIF
                     VNIVKMTFNLIMATHERIYTLLDDKEIFLREHEEVYDAIVGRDIDRAANLMVDHVRRV
                     YRTLQNGIAMEGVAAGRK"
     gene            complement(17428..18810)
                     /locus_tag="PK849_00080"
     CDS             complement(17428..18810)
                     /locus_tag="PK849_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPE64545.1"
                     /translation="MHISPTIFREYDIRGIADVDLTDQVVSAIGQAFGTWLVRQGVRK
                     IVVGGDVRLSTRRISGALVEGLLRTGVDVLNIGTVTTPVLYWSLHHFEVGGGVMITGS
                     HNPKDMNGLKLAFRGATLYGDDIQEILRMVLEDDMADAAHPGSLNEADIRDEYVRMLL
                     SKCSLGPKKLKVVCDSGNGTAGMMIGRYLETLGCEVVSLFDTPDGTFPNHHPDPQQRE
                     NLISLMDAVRREKADVGLAFDGDADRLGVVDERGEMIWGDILMALFWREILPKHPGAD
                     VIVEVKCSQSLIDDALKFGGKPLFWKSGHSVIKAKMKEIKAVFAGELSGHFFFADEYF
                     GFDDSFYAAARLLRILSSSDKTLSEMISDIPRYPSTAEVRINCPDEKKFDVVASLRDK
                     ALEEYEAITVDGVRILYPEGWGLIRASNTQPVIVTRCEGRTEADLETISADVRRRLLA
                     EGLPDFSWTY"
BASE COUNT         4424 a         5107 c         4810 g         4711 t
ORIGIN      
        1 atggccacgg aagagcgccc ccttcaaggc gggcatatgt tattccagcg tatgcaggag
       61 ctatacggcc gatggcgtta aaaattgtct cgggtgaatt catatcgttc catgaagcgc
      121 cgagtcgttt acctaccgca agcaggatct cccagtcggg acgagattcg ccgggagagt
      181 cgacagcttt gcggactctc tgtactgcgc ggcaagtgtt ggtaaacgtt ccatccttct
      241 cgccccagct cgcggccgga agaacaacgt gtgcgaactc tgccgtctcc gtaaggaaga
      301 tgtcctgcac taccagaaaa tcgagttttt tcagggcatg ccggacgtgg tcagagtcgg
      361 gttcagtgac catcgagttc tcgcccatga tgtacatgcc tttgattttt ccttcgccgg
      421 cggcatgcat catctcgaca acggaaaggc caggagtcgc agggagatca gaaccccaga
      481 gctccttgac cttctgctgc gtcgcaggaa gggtgacttt ctggtatccg gggatcactt
      541 ccggaaggca tccggtatca caggctccct gcacattgtt ctgtccacgc aacgggttga
      601 ctcctgttcc ggggcgtccc agcatcccgc agagaagcgc aaggttcgcg acggtccgga
      661 cgttatcagt accgttaaga tgctgcgtaa tgcccatcgt atagaagatg gcggagtttg
      721 gccctttcgc gtacgcacgt gcggcatcct caatcaactc gggggaaacg ccggtaattt
      781 ccgaaacgta ctgcggggtg tacttggaca tggtttcctt cagcttctca aagttattca
      841 cgttcttttc aataaaatcg gaagcgtaga ggttctcgcg aagtataacg tgcatcaaac
      901 cattcagaag tacaacgtcc gatccgctct tctggcgtat ataaacgtct gcgtactttg
      961 ccatttctat ctttcgggga tcgcacacga tcagggtgca gttccctttt cttttgcgtt
     1021 cctttatgaa agagccgatg acagggtggt tttcagtcgt gttcgagccg ataataagaa
     1081 tcgtatcggc agatttgata gattcaaagt cgttcgtcat agcgccgctg ccgagggtct
     1141 cgccgagacc cgtcactgtg gcgctgtgtc acagatgggc acagtgatcg acgttgttag
     1201 tgcccatcac ctcccttgca aggcgctgca taagatagtt ctcctcgttg gtgcaacggg
     1261 ctgaggagaa aaatccaagc gcgtccgagc cgtgttttgt ctttacttcc ccgagctttt
     1321 cagccacaag agagagcgct tcgtcccagg acgctttccg gaaggtgtcg ccttcacgaa
     1381 taagcggggt ggtcaggcgg tcctcgctgt ggacaaactg ccatgcgaag cgtcccttca
     1441 cgcacgatct tcccttgttc aaagctgtcg gactggagta attcgtggtg acattggcga
     1501 ttttgcctgt cttcttgttg acgttgacct caagctcgca tccaacgccg cagtaggaac
     1561 agacagtctt gaccttttca agatcccacg gacgcccctg tcctgccgct gttttttcga
     1621 agagggctcc tacagggcag acctggacgc actgcccgca gaagacgcag tcggactcct
     1681 ctatgggcga acccacagga ggctggatgg aggtgtggat tccacgcccc tggaaatcga
     1741 tggcgtggta gatggcgtgt tcttcgcaca cacggacgca gcgtccgcag aggatgcatt
     1801 tattcaggtc gcgaacgtaa aacgggttgg catcctccag cttgtgcgaa gtatttgtat
     1861 cgccggggtc tgcgaaacgt gaatctttga cgccaagttc gtacgccaca tcctgaagct
     1921 cgcacttgcc gttgctggcg caggagaaac actccagggg gtggcggaca aggatgagct
     1981 caagtacagc ttttcgcgcc tcgataactc gtggcgtgtt cgtgtgcaca atcattccct
     2041 cggtaatggg cgtggtgcac gaggtaagaa gtttagggtt cttctcggct tccacgaggc
     2101 aaatgcggca ggcgccgaag gggctcagcg cagggtgatc acaaagagtc ggaatgtgga
     2161 tgccgttagc ccgggcggcc tgaagaatcg tcatccccga ctcggctgtt atcgactttc
     2221 cgttgatgat cgcttttatg cttgccattc tctgatcatc tcctatcctt tgactacagc
     2281 gcccacgggg caattatcca tgcaggcgcc gcacttgata cacttgtcct gatctatgac
     2341 atgaggttcc ttcaccttgc cggaaataca gctcacgggg caattccggg cgcacttcgt
     2401 acagcctatg catttttccg tgttgatgga gtacctcaac aggctctggc agaccccggc
     2461 agggcatttt ttatcgaaaa tatgcgcttc gtactcatgg cggaaatact tgagcgttgt
     2521 gagcacgggg tttggagcag ttccaccgag ggcgcaaagc gaggcgtcct tgatctggtt
     2581 cccaaggtaa agaagtctgt caatgtcttc aggtacgcct ttcccttcgg tgattcgggt
     2641 caggatgtca agcatcttct tcgttccctc gcggcacggc acgcactttc cgcaggattc
     2701 gcgctgtgtg aattcaagga aaaacttcgc cgtgtcgacc atgcagtttc cctcgtcgag
     2761 cacgacaagt ccgccagaac ccatgatcgc gccggcagcg gtaagcgatt cgtagtctac
     2821 tgcaagatca agatgttcgt cgacgagaca gccgcccgaa ggacctccga tttgcacggc
     2881 cttgaacttt ttgtcgccga taataccgcc gcctatttcg aagacgatct cgcggatcgt
     2941 gatgcccatg ggcacttcga caagtccggt attcttgacc tttccggtga gtgcaaaaac
     3001 cttggttccc ttggacttct cggttcccat tgcggcaaag cactctgctc cctggacgat
     3061 aatccttggg acgttcgccc acgtctcgac gttgttgata ttcgtcggct taccccagag
     3121 tcctttgaca gccgggaaag gcggacgggc gcgcggcatg cctctctgtc cttctatgga
     3181 ggccataagc gcggtctctt cgccgcagac gaaagctccg gcgccctcct tgatgtgaag
     3241 ggtgaagttg aagcccgttc ctaagatgtc ttcgcccagc aggccatatt cggtggcatt
     3301 ctttatagcg gtctgcaggc gattaattgc cagggggtat tcagcacgac aatagatgta
     3361 tccttcgtcg gcgcccatgg cgtagccgcc gatcatcata ccttcgatga cggcgtgggg
     3421 gtcgccctca agaatggagc gatccatgaa cgcgcccgga tctccctcgt ccgcattgca
     3481 gatgacatat ttcttatcgc cgggggacgc tgcgcagaaa ccccatttca ttcctgtcgg
     3541 gaaaccgccg cctccacgcc ctcgaagccc ggactttttc atctcgtcaa tcaccgctgc
     3601 cggggtcatc tcgacaagac actttcccag agcttggtat ccgtctctgg agatgtactc
     3661 gtcaatattg tccgggttga tatacccgca gttgctcaat acgattctat gctgtttacc
     3721 gtagaacgga atatccttgt agtgacagac cttctgagcg gacagaggtt ctttgtaaag
     3781 aagacgtgtg acgatgcggc ccttatacag atgctcttcg acgatttctg caacgtctga
     3841 aggcttaacc tgcgagtaaa acgttccttc cggatagacc acgacgatcg gaccgaattc
     3901 acacatgccg tggcagccgg ttgcaataac aaggatctct ttttcgagac ctttctttgc
     3961 aagctcttct ttaaacgctt caagcacctt cggggcgccg ccggaagcgc atcctgttcc
     4021 gtgacagatg agtacgtgcg cgcggtacaa tgccatatcc ttatccctcc ccgcttagtc
     4081 ggctctgccg tacagcttgt cctgcacaat gcggccgttt acgatgtgct ccgagacaat
     4141 cctggaaacg tcgggagggg tgacagcgca gtaggttatc ctcggctcgc ccggacggat
     4201 aacatccaga agcggctcgt tctggcacat gccgatgcac cctgtcgtct ctacggcgac
     4261 gttgtgcaga ttgcgaactt caagctcttt aagcaccgcg ttcattattt cgcgggcgcc
     4321 ggcggcgata ccgcacgtac ccataccgat gatgaccttg actttgtcca cctcggaacg
     4381 cgcggatgtc aaatcgcccg ttttctcttt gatctttcgc agatcgtcga gactccggat
     4441 ctttttcttt tcctcagtca tttctttttc ctcctgtccg cattatttcc catttttttc
     4501 acatataaag ctcgccgata ttttccgcga tgtactcctt tactccaacg gcaagcgcgg
     4561 gatctgtgaa agggttcccg cccccgagcg catccttcag ttcatcgctg tccagaagga
     4621 actccctctt gccaactctg tgaacatatt tccaggagat atgcccgtga ccgacaagaa
     4681 gagtaagcag agtcgaggga atatctccgg gaggaggagt atctacgcac tgtttgctga
     4741 atgacgccga aacagttgtt ccttttcctg gcgctgaaga aagaaggaat tcaccaccgc
     4801 ataactcaga aagttgttta aggaaaggaa gtccaagacc cacccggcgc tccgtccgcg
     4861 ttgtgtagaa aggatccact acagcgcggg ctgtcgcttc gctcattccc ctgccgttat
     4921 cgcgaacggt caaacgaatg aactcgccgt cgtcgtcaaa tgtaacaccg atcgattcgg
     4981 ctccggcatt cacgccattc tcagcgatat cgagaatatg gtgggagaga tcctcaagca
     5041 tgatcaggcg tactgatcaa ggaattcttg caaggccgct tcgtcgtcgg tgagtctgcc
     5101 gtgagtatcc tcgtcgatca tcactacagg agcaaggccg cagcaaccaa gacacgcgac
     5161 tggttcaagt gtaaaacgaa gatcatcggt ggttccgttt tcctttacct tgagcatcga
     5221 catgattttg tccatgatct ttgcgctgcc gcgaacatga cacgcggttc cgcggcacac
     5281 tcggataata tggcgtcccc tgggtttcag atgaaactgc gcatagaagg ttgcaacacc
     5341 gtaaagctcc gcaagaggaa cgcccaactc ctccgaaact acctccattg cctcccggga
     5401 caagtaccct acttttttct gcgtctcctg aagcaccgga ataacacctc cgtgctttcc
     5461 tttccacgga gcggtaattt cctgtaccat ggaacgcacg tcctgctttg tttccacaac
     5521 cattgcccga ccccctaagt atagtgaatt taataacata tagaataaca aaaaactatc
     5581 tcttgttgat tctacacaaa aaaacaactt cggggaaaga aaccgttttc ctgtaaacgg
     5641 cgcccgatgt aaatattacc acagaaaaaa gattttttct ctaaaaaagc aagtttttgc
     5701 tttatctcat acatcttctc gatacgtagt cttcttgctg gatccttttt tacaaaaaca
     5761 catctaattc aataaaaaat aagagcatta agatttcagt ggaagatatt taccttcaga
     5821 gagcagcttt aatgcctcct tcactttttc ctgttttgaa agaacattcc agtcctgtct
     5881 gctctccaac tttaccatta tgtcctcagc agcgtcggcg agaccgaggg catctgcaag
     5941 caccagcatt ttctcggaat tctctgccgg gaagagcgat gcggccagac caagcaagat
     6001 tgtccctccg gcaacccaat ttgccaacgc ggcgtctcct tcagtcaacc acgcatcttc
     6061 aaagagcatt gcgagactgt agctttccag ttcctcctct tcagcctctt catccggttc
     6121 ttcatagtag ccgagaatgt ggaagggaac gtcgggatcc gccgcgaacg cttcccaaag
     6181 cgccctatag gccggcatgg tagctccgga gagcctaaac gcagctatag ctttactgtg
     6241 gagcattgca ggaaaaggct ctctttcttt caagcactcc tcaaggatcg ccgtgtattc
     6301 gttcatatcg acaaggatac gatagtaaag agttcttccc agcgtctgtt gttccgggtc
     6361 aaatgccatg attgagtgcg caatttcaag cgctttttcg ttctcgcctt cggagaaata
     6421 ggaaaaaccg aggcgttgaa gaactgctgc gtagagcatt ccgagttcgt catccagaaa
     6481 ggctgttcct tccggaacgg gcgattcttc cagatctttc tccaataagc aacttgcttt
     6541 ttgcaaaagg acgatcttct ccttcgagtc ctcggtgaca tcggccaaaa ggatgattgc
     6601 ttccacatta tctttgtcgt aattcaaaat ttgtcgagcg aggcgttcca tttcctccgg
     6661 atcatcggta gtatacgcct cgtcaagaag tctttcgatt ttctgtgtag tattcataga
     6721 tatttcctcc gtttgctttt tcagtctctt ccctctccaa gaagtctagc tcacgatttt
     6781 ttcccggtca aataggttgg tgtagaatat gaagaagtgc aaaaaaccat gaattttttt
     6841 tctaggagga gagggaatga gacgggtgcg cgtatctttt tctttgtgtg ttgtcttctt
     6901 gttacttatc ttgaatttga cggaaaggcc atcgttcgct tcaactccaa aaggtcgaat
     6961 tgccgattcc gtaaaagtgc ttcgtgaaat gtcgaaacag gatgatgcat cgacaatggg
     7021 agagcttatt gcgcaggcca ggggagtcgc tattttccct tccgtcgtaa aagcagggtt
     7081 cgttttcgga ggacagtacg gcgagggcct tgtccttcgc cgcgacccgg taaaaaaaag
     7141 atggtacggt ccatccttcg taagcgttac cggggcctcc tggggattac agattggagc
     7201 gcagtccata gcgcttgttc tcgtaatcac caacgacagg gggctggagg gctttatggg
     7261 aaacaacgtc aagctgggag gagatctctc ggtagccgcc ggacctgtgg gacgaagagg
     7321 tgaaatggga accgactata agatgaaagc ctcgatttat agttattcga tgacaaaggg
     7381 tttgtttgcg ggtgtctctc ttgaaggagc agctatcaat gtggacagga atgcgaacca
     7441 ggcgtactgg ggcaaaccgt tttcagcagg aaacgcctta aattcggctg caacaaaaaa
     7501 cgagataaaa cagctcgtca tcgaactgga aaaaatcatg aaacgggcga aataagctcg
     7561 aaaagatatg ctgccggcgc ttcgattccg cgcccgcagc atatcgtaca gttagataat
     7621 aagctttcct ccgccctgcc cttttcctcc cattctgttc agctgttgta gaacagccgg
     7681 agaggcgacg tctatcttcg gtttctgagc ctgctgtttg gccattgcct cctggacctt
     7741 gcgctcgtac tcctccatcc ccttcttcaa agattcaaca tcacctttga tccactggag
     7801 aattccgtcg gcgatggtct ccacgatgtc gtcggaaggc tcttcggcta tggcgccaag
     7861 ctccttcagc atgcggtgct cttctttgat cgactccact atatccgcgt ccgtgagaat
     7921 ccccttttta tacaaaacgc gtgcgacgat attgaacttg ctctttgaat tctcatcgtt
     7981 gatcgcaagg ctatccaccc tggcgaggag cattgagaga atttcatcga gacttacctg
     8041 cattggcatg gccattggaa aagcgcccct ttctaggaaa attagagtac tgattgtatc
     8101 acattagaca ttgaagcgga tttctatcat atcgccgtca tttacgacgt agtctttgcc
     8161 ctcaaggcga agcacgccct tttcccggca ttttgctaaa gattgctcgt gagaaataaa
     8221 atcttcgtag gagacgacct gcgcccgaat aaaaccacgt gcaagatccg aatggatcgc
     8281 tcctgcagcg tcaaccgcat ttgcgccttt tacaagcgtc catgccctga cctcatcctt
     8341 tcccgtcgtg aaaaacgaga gaagcccaag aagggcgtag gcttcggaga taaggcggtt
     8401 ccggcccgat tcttctatgc cgagttcgcc cataaactcc ttctgctctt cctcgggaag
     8461 ttcagtaagc tccatctcca ttttcccgaa gatcctgagt aaacggatgc cttttgtctt
     8521 cgccagctcg cggagtgcgt tgagtagagg aagcgaatca tcggtctggg tttcgtcgag
     8581 attcgcgacg atgagttccg gcttcagcgt tacaaaagca aaaccgctga gaagacgcaa
     8641 ttcatcgggc gaaagcccga gttctctcaa agggcgctcc tcaaggaggc aggcttggca
     8701 gcgttcaagc agcgcttttt cctccgtttc ggaaggaatg ttttttttct ttgcctgcag
     8761 gcgggaaatc ctgttttcaa taacgccaag gtcgcggaag attaattcca tctcgacgat
     8821 attccagtct cttacaggat cgatggaatt ttccggatgc gggacgtcgg gattctcaaa
     8881 aaggcggaca acgtggacca atgcgtcaga ttcggagaca aaagagagaa aagcatttcc
     8941 taccccttct cctttgctgg cgttgcgcga aagacctgca agatcgacga attccacggt
     9001 cgccggagtg acttttttgg gctcgaacaa gcgtgccagg tggtcgaaac gatcatcggg
     9061 gacgttgacc attgctctgt tgggatccgt cttcccgctt gcataaggct taacttcggc
     9121 gccggcacgt gtgactacgt tgaaaaccgt gctctttccg ctcagcggaa ggccgataat
     9181 accacatttg agcatttttc ttcctccttt gttgcgagac agtttttttc agtcccgtga
     9241 tgcattatac cttttccacg ccgaaaggga agaatcgcgt gtactttaaa aaagagtcaa
     9301 tccggttttt caaatttctg ttatcattga ttgcaagctt ttataccttg atcaaggagg
     9361 tttttcgaga tgaagatggg cgtcttcaat gtatcgcttt tgcttttttt tctgttgtgc
     9421 gtcctaagcg ttccctccga ggctgtctct gagtattcga ttctccgtac agatatgtac
     9481 cctgccggcg cacggttggt cttttctatt ccggcttcgg aaaaggtgca tttccagctg
     9541 cccggagctt tcgacagcgc ttcggttcgc ccgctcccct ctgaaggggt ggaagttctc
     9601 tctttcgaag ccgttgacgt atcgcgttcc gaatggattc ctcccgtact ggagaaactt
     9661 tttacgtcta ttcgtgaaaa agaacgacta caagcgactc tggggggcaa gatttcggcc
     9721 atcgagaact cgctggcgct cctttccgga cctcttccaa aggaattaaa aggcgccgat
     9781 gtagcggagt acattgaatc agtccgggca gtccgggaaa aaatggaatt ggaacgcgct
     9841 tctctagcgg acactttaga tgaactccaa aaggaactcg ccgttcttca agcggatttc
     9901 aaatcgaaaa tgcccccgga ctctcaaaaa gcggtagaaa ttacggcagt tctttccggc
     9961 agcggcgttt ttctcgtgga agcattcaca cgctacgcgg agtgggtccc cttttataga
    10021 atgaacctct ccgtaagcag cggcggtatt tcggccggac tttttgcgcg agcgcggcaa
    10081 aagacggggc ttcttgtcga agggaccgta cacttccata cgtcgatgcc ttctgtttcc
    10141 gtggcgcctc cggaactgcg tccgctcgtc gcggatctgg ctccgaaaca caaagatgtt
    10201 cggcgtttcg ccatggacga agctctcccg atgatggccc caatggtgca ggctcccgcc
    10261 cccgcagaac cggcgcctcg ccccgttatc cagacgctta ccgacctttc aactcaggct
    10321 gagggactgc tgacggggga ttatactccg gcagatttta acctgggcag cttttcgctg
    10381 aagtcagaga cttctattat ttccgtcccg tctctctctg agcaggcatg gctcgtagca
    10441 gagtccaaag ggcttccctt ccctatacta ccagcctccg ccgagctctc cgtcgacgga
    10501 agtccgtccg ggaaaacaac gcttgatgag cacggctccg gttccgatat tcttattgcc
    10561 ttcggcagaa cgcctcgagt caaagcttcc aaggagaaac tcgtctcaaa ggaaggaagc
    10621 acatggacgg gaagaggacg caccgaggac gggtatacga ttgaagtcac aaacggcatg
    10681 ccccatcctg tcgctgtcac gctgaaagac cgtattccaa tttcatcgcg ggaaaatatt
    10741 tctgtcgaag tattgcgcat tgaacccaag ccggacgaac agtcagaaca aaatctcctg
    10801 acatggaagc tgtctctcgc tcccggtgaa aaacgtacga taaatgcggt tttccgcttg
    10861 agctacccat cagatgagac ggtgattttt cgttgaaata ctacattctg cggtactcgg
    10921 cgtccaggag aagcaaacat ataattagga gaatccagcc atgattttat ggctcaccat
    10981 acctccgctg ctttcgctgc ctctctctct ccttctcgtc gctttcggaa atgagagcaa
    11041 caaagtatcg gcgggggccc ctttgtgggt gcttttactt cttttgtatt ttgcgttcag
    11101 catgttctat gttttttcaa taaagaacag cttgttttcc caattaccac cgcttcagat
    11161 ggcggcccag ggaatcatca tatgcatcgt ttccatggag gcgggcgctc atccgattct
    11221 cccctgggta ggcgtactcg ctgctgtttc tggttttttt atattgttgc actcgtactc
    11281 gcagtacgga aggggaacac atcttcctcc gccggtcgcg cacgctgaat ccccttcctc
    11341 tgatacagca tccgaacctt ggataaggtc tcctttccca tacgttattt tggataacga
    11401 ggggacaatc atcgatgcaa acgacgcctt cgcgtctttt gccgaaaacg gttctgtaca
    11461 aggggaaaat gtgacaacct tttttgttcc cggtgagaca gaagctgttg tcgggggaaa
    11521 gaagcatgca atttttcaga aggcgaagga cggtcagttc cattttttac ttgttgacaa
    11581 ccctcctgcg caaaaaaatt caaagaccgc tgaagcaaag agcgagctcg aactctttga
    11641 cccgaaatac ggactctact ccaggaaata cgcccagttg cgtatcccgg aggaactggg
    11701 cagagcaaac aggtacagac gctggctttg cgggatttta ctcaaggtga actacaccta
    11761 ccttcccgga agaggccggc agcctgccct ggaggaaact ttccttgccg cgtatgttct
    11821 ctacgtcaag gagtccatca gggagagcga tatgggcttt tccatgcagg acggggagtt
    11881 tctgctcctt ctccctgaaa ctccacagca aggcgcaaaa gacacggcac tcaaactaat
    11941 agtagttccc gaatcgcttg atgagatgaa gaaggactat cctttctcgg tcgatataga
    12001 gtatggattt ttgtattaca gcgggaacta tcctctttcg tatgagcagt ttatccaaaa
    12061 gctttatgct tctttaggag ggagcgctga atgattactt cgaaaaaagc tgtctgcaca
    12121 ttgctcctcg gccttttttt tctgatcccc ggatttatgc gcatctcgga agcgaatacg
    12181 cgagatcttt cttctatgac gcttgaggag aaagttggac agattatgct ggtttttttc
    12241 gagggaggag caatgtccga gaaccttcaa aagttcatct cggagatcaa ggtcggagga
    12301 gtcatcctgt attccagccg aaacaacatt gagtccgccg aacaggttgc gaacctgaac
    12361 gagtcaattc aacggagagc tctcgcttca agttctcgtc cgctcttcat agctattgat
    12421 caggagggag gtctgattgc ccgtatccgc gagggtgtca cccctttccc gggtaatatg
    12481 gcccttggag cggcagggga tccgtctctg gccggtcgtg tcgcttccgt aatgggggaa
    12541 gagcttgcga gcctcggtat caacatgaat tttgccccgg tggtggatgt gaacaacaat
    12601 ccgttgaatc cgataatcgg cgttcgctct ttcggctccg acccgcagga ggtcgcccgc
    12661 ctcggggcag caatgatttc ctctttttta gaaaaaggcg tattcccctc tgcgaaacac
    12721 ttccccggac atggagatac cgaggtggat tctcattctg gcctccctat catcaatgtc
    12781 tccaaggatc gtctgaattc tgttgaattc ccccccttca aagcggctgt caatgccggc
    12841 gtgccgatgg tcatgactgc gcacgttctt gttcctgctc tcgacgagaa aaatccggcg
    12901 accctttccc ccgccatact cgggatgctc cgcaccgaac tgaacttcaa aggcctgata
    12961 atcacagact ctatgggtat ggcggcgtta aaaaaagaaa ggaccatgga ggaagctgct
    13021 atagaagcga tgaacgccgg agcggatatc ctgcttttcg gagccgataa agggcatgaa
    13081 gaaacggagc acttcaacat gtacaacgca ctgttgaaag cctgcgttga aggggaaatt
    13141 ccaatcagtc ggctggatga cgctgtacga aaggttctgg aggcgaaaga agcgcttggt
    13201 cttcttcaag atgaatggga acctcacttt gctccgcgca tcgctcccga tggggctgct
    13261 accgcacgcg aagcggcgtt gcgaagtatc actgttgcca ggaacagatc tgaaactttg
    13321 caaaccgtac gagacggaaa aaaacgcccg ctgctctggc ccggggaacg gattgctgcg
    13381 ggaaaagttt tctgtgaatt tctcttcgat gtcgacctga tcgaagtccc ggaacggccg
    13441 gaatctcatg atatctccgg tatactgcag cgcgtagagg gcgtcgacag gattctggtc
    13501 gcggagtacg atagtaagag aaacccggac tggctcaacc ttgtccggca gatcggtgaa
    13561 gaacgaactc tcctcatctc tcttcggact ccgtacgcac ttttatcgct gccctccgtg
    13621 tcgggcgcta tcgtttcata tagcgacaac accccttcgc ttcaggccct ggccgatgta
    13681 ctccggggaa acgcccccgc tgaaggaaga ttgcccgtag agcttcccgg ttttgaaata
    13741 cgaacgggag gatgccgggt aacctccggt ttttaagcta caccgccagg gagagctggt
    13801 gttttacagg aaatgatgat attgccgcag agatggcgcc gggttccgaa aggaaccacg
    13861 cgccattttc atttttatcc catacgaggg attgggtgca acatccgctg caaagaggag
    13921 cgtcacagaa ccaattcccg tttgtatctt tcatccacat gttgaaaacc tcctcgtgtt
    13981 tttgtctgct tggcagcgac tgacactgtt tctttcacga ggatgttata ccacgacttt
    14041 tttcataagt caacacccgt ttagcttttc tattcaaaaa aaccttagtt cattttcccg
    14101 aagagtattt tttctcccgc gggatcccgg acaagcggaa caggcattag gcgggttggg
    14161 aagacatctt tccccgacga cgctttcata agaaggcgac cgacggcaac tcccatttca
    14221 aaagccggcc attcgatacg aaagaaacct gtacgtaccg gttcctcagg gagagtacag
    14281 acacatccga aacgaaccgt acgcccgacg tcctccggtg cgcaaccatc catgatccaa
    14341 aagtattcct ccggggctgc ttccgaatct gtgatactgc gtgagagaaa ggggaaaaga
    14401 ggagacgttc ccgaaacaaa acgaagcgga cgccccgaag agacggagcg aagccgtgag
    14461 agcgtctctt gctctgaaac gaaaactgaa ggtgtttcag caatggaaaa accggccgtt
    14521 acaacaggta tctgcggtag caaaaagcgg gagggcgcca tcccctcccc gcttcctgca
    14581 agaagaatac agtcgacacg gcgtgataca agcgaagctt ccagcgcgtc atcgctgaaa
    14641 ccgggcatct tgagaagaat atccattccc gcgttgccga gaacacggtt caatccagaa
    14701 aagaaaagcg gactgaacca tggtctaagg ctttcaagca caactgccgc aaggccggtc
    14761 ttgcctccgg aaagcccgct tgcggtacgg tcaatacgat acccgagagt cttcgcagca
    14821 ttccacactt tttcactcgt gacggaagag atgcgaggat ctcctttcag cgctctgctg
    14881 actgtggcct tgttcacacc ggcgaaccgg gccacatctt ccatcgtcgc tcgcgtcaca
    14941 ataggaaggg gggcgctaca cgtcgaaaca tgagccgccg atgaactccc tgataatcga
    15001 gttgttcggt atgatctccg ttggaatcat gggaacaaac ttgatcatcg agagaattcg
    15061 ctgcgcttca ccgtagacag gggagttttc cgggaccgta tgaagtatcg gatcgacata
    15121 cgccctcaga gccgccaatt cgtacggtag agaggagtta aacatcacgt ccgcatgttt
    15181 ctggtagggg aacacaaact gcatgctccc cctgatgacc ttcggccaga gggttaacgt
    15241 tgattcagca gaatgccctc tcgttcttgc atcgcgtaca agacggcgaa gaagacggtt
    15301 gtccgtggta ctcgtccggt tatggttatc gaagttcaca cctgtgagcg gagagacgaa
    15361 aattccgtac agttcgtcct ttgcaatcgc gccggtcacc ctgtcattca agccgtgtat
    15421 tccctccatt atcaggatat cacggggacc gagcttaatt tttttaccgg gttttttctt
    15481 tccctcgata aaactaaact gaggggtgac aacctcttct ccggcgagaa ggcggcgaag
    15541 gtggtcctcc agaagttcca attcgagcgc ctccactact tcaaaatcga ggttgccttg
    15601 ctcgtcgcgc ggagtatcct cccggttcac aaagtagtta tccatagcaa gcgtcacggg
    15661 atttctgccg cagacgagga gctgtatttt cagcctttcg gaaaaagttg tctttccgga
    15721 gcctgaaggc cctgcaatcg ccgctacctt cacttttgtc tttgaggcta tttcatcggc
    15781 gatccgcccc aatttctgcg catggaatgc ctctgcgata aggataactt cttgacttct
    15841 gccctccgca acctttttat gcagactgtc cattgtccgg agatcaagca cttcaagcca
    15901 tttagcatag tccaggaaaa ctccggagag atttttcgaa gcgcggaatg gaggcagaga
    15961 ggaaggatag ctgattgtcg ggaattgaag cgccatcccg ggtccgagct ttttgagatc
    16021 gtagagcttc aaatacccgg tcgacggggc gagcggggca tagtagtatc cgtacatatc
    16081 tgcgcaacga tagagttcca caggatcgac ggcgccccac tgaaagagcc gtgcaacttc
    16141 cccgcttccc tgccggtcga aaattctcct ggccttatcg aggggaacaa cttttcgaat
    16201 aaacggcaga tcctttccga caagatcagc catagtggcc ttgatgaccg caaggtcttc
    16261 atccgtgact tctccctcta cgaactccca gtagtaccct tcactgatgg agtggcggag
    16321 aacgacgtct ttcgccaagc ctcgtttaca tgcaagcacg aggaggaagc tcagggagcg
    16381 gcgatacact tccatgcctt caaaggagga tgtatcaatg aactcaaccc gtgcattgtc
    16441 ttcaataacc cagtcgagtg aacgaaggta attgtttact ctccacgcga cgatggtctt
    16501 ctcttcgccg tctgggcggc tctccgaaag aacggatttt cctgtgaccg gaacgtcgct
    16561 ggaaaaaaca ggcccgtttg caacttcaat agtgaatgcc atagaacgtt ctcctccctt
    16621 cgataaacgg gttgcgctgt atttgaaagt agattatgct tatattacca tacggaacac
    16681 aggaaggaga cacacatgga aaacgtaaaa gggaagacga aaatatacga ccaggtggtc
    16741 gatagtatta aactgcgtat ttccgaggga gaactgcccc aaggctccgc gcttccgccg
    16801 gaacgccagc ttatagacga actgaacgtc agccgcagct cgcttcggga aggatttcgc
    16861 attctggaaa tgatgggttt gatcgaaagt attccgggca aaggccgttt tgtgaggacg
    16921 tcgcgaagcg acggcggagc gaccggccga gtcccgctcc aggacgaggc tatcctggaa
    16981 ctcgtcgaag cgcggcttgt gcttgaacct gcaatagccg tcgaagcggc aaagcatgct
    17041 tctccctccg acctcacgag aatacgccga attctaacaa aaactgggaa agacgtggaa
    17101 tctttggagc accgagccca atgcgatttc gattttcatc ttgttctggc ggagtcaacc
    17161 cataatttca tttttgtcaa catcgtcaag atgacgttca acctgattat ggcgactcat
    17221 gaacgtatct atacgctcct cgacgataaa gagatttttc ttcgtgaaca cgaggaagtt
    17281 tatgacgcca tcgtaggacg cgacatcgac cgcgccgcga acctgatggt cgaccatgtt
    17341 cgaagagtct accgcacgct tcaaaacggt attgcaatgg agggcgttgc cgcagggagg
    17401 aaataacagc ggcaacgcct ctgccgttca gtacgtccag ctgaaatcgg gaagtccctc
    17461 cgcaagaaga cggcgtcgca catcggccga aatcgtctcc aaatccgctt cggttcttcc
    17521 ttcacagcgg gtgacgatca ccggttgggt atttgacgcc cggatcaatc cccacccttc
    17581 cgggtagaga attcgaacgc catcgacggt aatggcttcg tactcttcaa gcgctttatc
    17641 gcgcagcgac gcgaccacgt cgaatttctt ctcgtcggga cagttgatcc gcacttcggc
    17701 agtgctcgga tatcggggaa tatcggagat catttccgag agtgttttgt cgctcgacga
    17761 gagaattcgc aaaagcctcg cagccgcata aaacgagtcg tcgaatccga agtattcatc
    17821 cgcaaaaaag aaatgccccg aaagctctcc ggcgaaaaca gcctttatct ctttcatctt
    17881 tgctttgatc acggagtgcc ccgacttcca gaagagcggt tttcctccga acttcagggc
    17941 gtcgtcaatg agcgactggg agcatttcac ctcgacgatg acatcggctc ccgggtgctt
    18001 gggaagtatc tcacgccaga agagcgccat aagaatatcc ccccagatca tctcgccgcg
    18061 ttcgtcaacc acgccaagcc gatccgcatc gccgtcaaaa gcgagcccta cgtccgcctt
    18121 ttcacgccgc acagcatcca tcaaggaaat caggttctcc cgttgttgcg gatcgggatg
    18181 atgattcggg aacgttccgt ccggtgtatc gaagagcgac acgacttcac atccaagcgt
    18241 ctccaagtac cggcctatca tcataccggc agtgccgttg ccgctatcgc agaccacctt
    18301 cagttttttc ggtccaagag agcatttcga cagcagcata cgaacgtact catcccgaat
    18361 gtcggcttcg ttaagagaac ccggatgggc ggcatccgcc atgtcgtctt cgaggaccat
    18421 acggaggatt tcctggatat cgtctccgta cagggtcgcc cctctaaaag caagtttcag
    18481 accgttcata tccttgggat tgtggctgcc tgtaatcatg acgccgccgc cgacctcgaa
    18541 gtgatgaagg ctccaataga gaaccggcgt ggtgacggta ccgatattga gaacgtccac
    18601 acccgtacgt agcagcccct cgacaagagc gcccgaaatc ctccgggtgg aaaggcggac
    18661 atcgccccca accacgattt ttcttactcc ctgacggacc aaccaggttc cgaaagcctg
    18721 tcctatagct gagactacct ggtcggtcag atcgacatcc gctatgccgc gaatatcgta
    18781 ctcccgaaaa atagtcggcg aaatatgcat tctacttcct ccttttgaca cgtcgctctt
    18841 gtcgtccgca gcgtctcgct gtatcatcga aacgcgtgtg cgtataccct tataatcagg
    18901 gtatacaaac taaaagtcaa gattcggtat gcgtattgca tagaaaagga tgtgctcctc
    18961 gtgaatgaac tcctcataat agcgcctatt gcagcagtga ttctccttgg gagattgctc
    19021 ggtacgagcg gcgcagtttc tccgcagacc tt
//
LOCUS       DAOPKU010000002        17945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_3305_length_17945_cov_5.220011, whole genome shotgun sequence.
ACCESSION   DAOPKU010000002 DAOPKU010000000
VERSION     DAOPKU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 17945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17945
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3305_length_17945_cov_5.220011"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PK849_00085"
     CDS             <1..619
                     /locus_tag="PK849_00085"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HPE64546.1"
                     /translation="FAGNAAGESAEYSDPARWLSLPSYTLMDTDIFYLYPTACRNEEA
                     AIRGVDDPCMAEGALELFEREIPFLEKLGDVYAPYYSQASAYRSSLPRERRRELVNGA
                     PRRDVFAAFDYYMEHYNLGRPFILVGSCQGAEVLIQLLSEYISKDSPIYGRMRAAYLS
                     DCDVPEDFLLSNPHLRLARTPDGDGFVISSAGDVSAVPGSELQKQ"
     gene            complement(681..2057)
                     /locus_tag="PK849_00090"
     CDS             complement(681..2057)
                     /locus_tag="PK849_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE64547.1"
                     /translation="MRGYTLKLFWWFWLTAAATGLAHHLTSVITEGNIFREQMIANVQ
                     EIALVYGERVASKEAREGPEEAVRYLERLRRTHRFFGFLFGEDGTELTGQPKAPESIG
                     KIVDEARNTGAPVVRMERGRPLFAVPLEEFPGLVFAGRILNPFFESRSLPVLRLIAAA
                     LVAGLLSWFLAVRLAKPVRKLRGAVQRLAAGELGARAEHTAKRNDEIGQLAKDFNRMA
                     DRIEELLRGQRQLLADVSHELRSPLTRLGVGLELLRRRSASPDDEAFKRIETELGRID
                     NLIGSLLKLGRLEALEQPERRARIDLTALLRQICADAAFEAAPLGKRIETDFAEGALL
                     HADQALLRSAIENVIRNALRYTPQHTTGQDGGKVRVSLFRVPETERPLHILVEDEGPG
                     VPPGELETIFIPFRRVERARERISASEGTGLGLAIAHRAAALHGGVISARNRPEGGLA
                     VEIELPET"
     gene            complement(2039..2731)
                     /locus_tag="PK849_00095"
     CDS             complement(2039..2731)
                     /locus_tag="PK849_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE64548.1"
                     /translation="MHRILLLDDDIELCDLLTEYLRPEGFEILVAHDGEEGLAAALSS
                     PVDLVLLDVMLPGKNGFDVLRELRAHSPVPVLMLTAKGDEIDRIVGLELGADDYLPKP
                     FNPRELLARIRAVLRRGERPETALSERLSAGDIEMDLGARTVRVAGKDTELTSVEFKL
                     LEALLRSAGKVVSREKLAMAALERTLGPFERSLDVHVSNLRRKLGPADDGSSRIRTLR
                     SEGYLLACAATH"
     gene            2961..3830
                     /locus_tag="PK849_00100"
     CDS             2961..3830
                     /locus_tag="PK849_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HPE64549.1"
                     /translation="MKKMKKRILVGSLLFVLVVLLVGGVSQYAFASRMMGAGPWGGMS
                     HWHDRGDSAFKAELLRALNPMDFTEEQWKKFDEILGGLRDQGKNFRRGMMKEMWNTRK
                     EIHAMMTADGFDEEKAKEMIAAVRPSFVSMAQEVLKARNELYGILNDAQRNKVGAVLD
                     VIGKRLEQPFSGPRELHAAMTRRLELTPDQKGKVEAITKETAPKMHAFAKTLFDQVKS
                     EHAALANGPMSDEQLAESAGKIADLAEGALMEMGKVRAQVWETLTPEQREEMGKMRHP
                     MGGMERRPGRMRK"
     gene            4156..5343
                     /locus_tag="PK849_00105"
     CDS             4156..5343
                     /locus_tag="PK849_00105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPE64550.1"
                     /translation="MTSSDFEYGNDPQLRESIERSILLGNLRRMQRLLWGLALFLALV
                     LLCSPFLYEKSVQHFGSAAPLEVQILWRSAWLAFDLFALFGIRRILSHPPERIGLFHR
                     AVETGVPLINMLFVVSLLAAGYAYNPSLEILYIALFAMAPLIRLNAAKTFIVVGMPLG
                     LFVALMDRRAAGVAHMSNMISLTAVVILAAIVFREMYRSTVREMEQQAIIERQLKIME
                     TFANTDGLTGLANRRSLDENLESEWRRGMREHNFLAVVMLDIDLFKSYNDQYGHPSGD
                     ECIRNIASVIKGYARRAGDIAARYGGEEFVIVLPGVDLGDAASIAEKIRLEIFSANRP
                     HAGSPEGRVTVSAGVASAPAASLDSGEALLRSADLALYRAKAEGRNRVVAASESARVV
                     AEK"
     gene            5382..5861
                     /locus_tag="PK849_00110"
     CDS             5382..5861
                     /locus_tag="PK849_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64551.1"
                     /translation="MSGRDDDLLAKELEKLAAAGGKIAAKISGKSSPAVTAEDSEIMR
                     KAARRLDTDQYREERMIEGDARSVLQLAYDALKNTGRILEENERESSVNPRISGVVGA
                     GFFGKNPAVVHAEIVGADERRCTLRLTGAAKEGLIKQGTARKAVEKVLREFDSLKSS"
     gene            6104..7060
                     /locus_tag="PK849_00115"
     CDS             6104..7060
                     /locus_tag="PK849_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE64552.1"
                     /translation="MSGIRKSMSPLEWLLLLFLSVLWGGSFFFVGIVVHALPRFTIVA
                     LRVGLAALALHILLLFRGERIPLDRKLWGAFFVIGLLNNVIPFSLIIQGQMHIASGLA
                     SILNATTPLFTVVVAHFLTDDEKMTAGRFLGVLVGFSGVVLMIGPEAVAGLGTHVVAQ
                     LCVLGATLCYAFAGVFGRRFRGMKLAPMIAAAGQVTASSIILVPLALVLEHPWTLPAP
                     APEVWGAILGLALLSTAFAYVIYFRILASAGANNVLLVTFLIPVSAILLGTTVLGERL
                     EPKHFAGMALIGVALAAIDGRLFGMLRRFFRTSPGDNPGDCE"
     gene            7076..8290
                     /locus_tag="PK849_00120"
     CDS             7076..8290
                     /locus_tag="PK849_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64553.1"
                     /translation="MFRRKMKGSLLVALFLLAALLPQAPRLEAAPPGTTDPFARAPFL
                     LMDEEAVVLTQESGNIYSDVFLPNVVQREDGSRAWSERLDDAKTTWSPRPPLLDGRVG
                     DAAVLLRNSRNEAFLAQVWNAISSTGLTMSLAPVDQNGYKAEIMRINSRWGFFSINYF
                     VAAAGDLNGDRATELVLVSTGDSNGPIFFTASVAGFDNPVGSEFSNAGEVPRIHWRSG
                     FRIATGDADGNGRDEIAVMYVSEAGKYVVALFKADENLRLRLLGVHTLDIAPYRDTYY
                     PPDQFDIAFADWEGDGRKKIFCVTGYAGDGYGRNVRLLSVTIQDGRATFTTESRHSHK
                     FFGDQGPILAAAADINGNGKDELLLTNRWGSPPSSVRNSSRCTTRATAPPEPPVSIGA
                     TIDSRSTGPSPI"
     gene            8200..10521
                     /locus_tag="PK849_00125"
     CDS             8200..10521
                     /locus_tag="PK849_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64554.1"
                     /translation="MYNKGDGSSGTASKYWSNDRLEIYRPISHLKTGRFTGSLHPSAA
                     AKGGSHAMVLLHQIHWRGDGTFARLLRLEGDTLKEVSEARLSGFSNPDRAHLLVGDLD
                     RDSMVLGYPTHITLENHVTPLLFMNEPPKHIDADGTGAVTNSTRRDDLYVQYAQSDSE
                     SRETTNTFEAESTLGGSVSVEQKGGIDLGIVKAETKLTTTVGGSTKDLRSEIARSYQS
                     RSTSLTGATTRDDVLLYLSNRVHLWRYPILGWMSEPEGGGAKVPTYYQLSIPEAEASM
                     KFLPGLNVDWYHPAHMNGNVLSYPSRRAQIGDFAEDRRLSDNVEFGVGGGTAFTRGIT
                     WKDSFTRESLKSVEVKMNLDVELQVSGSAQVEIFEVGGSTTASFHMDKRTTETSVAKT
                     SVEKEKAFTVNIPAFSTPLGYQITPLMYRTGGGVLKTSHVVDVLNNRSLWASRYAAPD
                     PALNLPRLWRYVEGDGWKWAAADPLNPDARKIRGIFFRNANGVDLGKSLPLGETTTIA
                     VRVHNFSLVECPEVDVLFEAVPYGGGPAVVIGTARTPRIDPWGASEAANWKWATVAWN
                     TSGMTEGNYTVRVTANPDGRVAELEGRALNEPFDNNLGWYDVFVGRSRSAVEEELAAV
                     NENLVAATLELANTPADGKAFALGEVALLRGTVRNEGVLPVGNISLFFYDGNPDGGGK
                     PIANRIVSGIVPGGEYSVVLTHSFDTPGPRSVFMRVVPKVGDPPEDNTVSVAVTVQGG
                     DSGGGGCAVGTAGAWSALLLIGLAPLAFGRKER"
     gene            10525..11718
                     /locus_tag="PK849_00130"
     CDS             10525..11718
                     /locus_tag="PK849_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPE64555.1"
                     /translation="MKNLGLIPKLIIGIIVGILIGKFGGVLPVRILATFNGLFGNFLG
                     FVIPLIIIGFVAPGIGELEAGAGKLLGMTAGIAYSSTVMAGLAAFFVNSALFPLFLTP
                     GSIVLDDPGNPEHALLPSLFNVEMPPLMGVMTALLLAFTLGLGMAAIRSGALQRGFKE
                     FQQIIEKLIKGIIIPLLPFHIAGIFANMTHAGQVAEILSVFLKVFLIIIALHLGVLVL
                     QYVTAGIVGGKNPVRLLRTMIPAYLTALGTQSSAATIPVTLEQSVRNGVRRGVANFVV
                     PLCATIHMSGSTITITSCAMAIMVLNGISFSFSTMLGFIMMLGVTMVAAPGVPGGAVM
                     AALGLLQTMLGFDQTLISLMIALYVAQDSFGTACNVTGDGAIAVVVDRIYGKLAEDEP
                     SGAAS"
     gene            complement(11819..13570)
                     /locus_tag="PK849_00135"
     CDS             complement(11819..13570)
                     /locus_tag="PK849_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HPE64556.1"
                     /translation="MNDTSAHIKRLLIFLYGEEEAERVLPELKALMDSYRGKIPAPSR
                     REYLPDERDAILITYGDQFRAPGEPPLRTLRGFLDTHLEGCVSGVHILPCFPYTSDDG
                     FSVVDYRRVDPELGDWDDIAALGERYRLMLDFVANHISRRSDWFASFLRGEAPYTDYF
                     ITPPEDADISRVVRPRTLPLLTKVETAQGERSVWTTFSADQIDLNYAAPRVLLEMTGI
                     LLHYAFRGAEIIRLDAIAYLWKTPGTSCIHLPKTHAVVRFWRAVLDDVAPHVLLITET
                     NVPHAENVSYFGEAREDGRGTDEAQMVYNFSLAPLTLHALLTGDATVLSTWASELSPP
                     ARGATFFNFIASHDGIGVTPARGILSDEQVRRLAERALAHGGHVSYKTNADGSISPYE
                     LNITLYDFLNDPAKPEPGTDAARFIASQAILLSLAGVPGIYVHSLFGSRNCHECFAET
                     GRARSLNRKKFDRSGLEEAIADPAGRPGRIFNELRRLLRVRREEQAFHPEGEQKVLDI
                     GEGLFALLRTPPEGGRPVLCLQNVTVSPMHVTISAGHLPGLDGSCRDLLTNEEYRTER
                     GVAEVVLNPYGTCWLTT"
     gene            complement(13627..14964)
                     /locus_tag="PK849_00140"
     CDS             complement(13627..14964)
                     /locus_tag="PK849_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPE64557.1"
                     /translation="MNASQETTALLQSAREVLDENWKDGFTVPAQNQYPYQWSWDSAF
                     IAAGYARYDRERAQRELRSLFSAQWADGRVPHIVFHNASPDASYFPGPEFWQTERNPF
                     APLSPRTSGIVQPPNHATAVLHIVRCAGEEGEKAALAFAGEMFPKLRAWHDWLYRERD
                     PRGEGVVYIIHPWESGQDNSPLWDPVLARMDVPPAHFSGFRRRDTRAVAPEERPLDSD
                     YDRYIFLVDFFRQRGYDERKIFGDGCPFLVQDVLFNTLLCRAEQDMAELARLLGENPA
                     PFEARARRTADAVNRKLWDGDRGYYADYDLASEKPIPMPALAGFSPLFARIPGEERAE
                     TMLRRLLTPSFGLGAEQKGYPASSYDRETPEYSPRRYWRGPTWIQMNWLLMHGLRAYG
                     RNDQAERLRRTIAELPAKGGFREYFNPETGEGHGAHRFSWTAALLIDVLYEQL"
     gene            complement(15083..16180)
                     /locus_tag="PK849_00145"
     CDS             complement(15083..16180)
                     /locus_tag="PK849_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64558.1"
                     /translation="MSEIRIENVVKRFGDFVAVNDVSLVVAEHEFMVLLGPSGCGKTT
                     LLRAIAGLGMADEGRISIGGRDVTYLPPRERRISMVFQSYAIFPHMNVYDNIAFGLRM
                     QKEEKTEIENRVRRAAELLHIEAMLDRYPSKMSGGQRQRVAVARAIAMQAQVLLMDEP
                     LSNLDALLRLEMRAELKQLLAEIGTTTVYVTHDQIEAMSMGDRIAVMHGGTVQQVDSP
                     TTVYDYPANRFIGGFIGNPPMNFLEGQVRRKDGDTFVRLGEFDVTPTPRMQPVLKEYD
                     GKPIVLGIRAENLETLHEPAHDALRTDVLVVEPLGSQNLLTAAFGGHRIKVSTHPGFP
                     ARAGGEVWLRFPPESVRWIDPDTKRALYPSL"
     gene            complement(16206..17069)
                     /locus_tag="PK849_00150"
     CDS             complement(16206..17069)
                     /locus_tag="PK849_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPE64559.1"
                     /translation="MSVRVSDEMTKVRARAKRRLVIKRALTYLTAIVLSLWILLPIWL
                     IASMALTTPEAMRNFPKGVLPFIPFSLDTMRFFLEARGIIPGLINSVVVALITLALST
                     LIAAPAGYALSRYVFRGRNAFRLSILAVRAFPVVILSIPLAVTFINLGMFDKVSSLAL
                     MHTALTLPTTVLVIGSVFASIPYELEEAAQVFGCTAFQAFRRVVLPLVLPGIAAAALF
                     TFVMSWNEVFAATVLTVQNRTLPAQVLVSLREAPDAYKFAGGFFMLIPSVIFIMIIRK
                     YLFNMWGQSSK"
     gene            complement(17066..>17945)
                     /locus_tag="PK849_00155"
     CDS             complement(17066..>17945)
                     /locus_tag="PK849_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPE64560.1"
                     /translation="IAQRTSLEVWFLIEGVRNEQTLSGWAPSRRLQIYGDGATGRVDS
                     GDTGRFTLLHIQRMLNDRFFRSALGTTFLLMLLIIPVQLVLAIVMALVIQARLKFNLL
                     FLAIFAIPLAASDLAVGIVWSSIFTQNGYLNSILYSLGLIESPQAYLTAATRHWILVA
                     VWIAEVWRATSLVMVIVVSGLQAISQEVLEAAELFGATLWQRVRYVILPLLKPSLQVA
                     LILRTILALQVFAVVIALGGGDVVTVLANETYRQYYALRNMNVAAAYSGLILLLSMIS
                     AGIYLRTVRGQEEAVS"
BASE COUNT         3354 a         5436 c         5761 g         3394 t
ORIGIN      
        1 ttttgcaggg aatgccgccg gcgaaagcgc cgagtattcg gatcctgcgc gctggctctc
       61 gttgccgagc tatacattga tggataccga tattttctat ctttacccta ctgcatgcag
      121 gaatgaagaa gctgcgattc gcggcgtcga cgatccgtgc atggcggaag gagcgctgga
      181 gcttttcgag cgggagatac cgtttctcga aaagctgggg gatgtgtacg ccccctacta
      241 cagccaggcg tcggcctacc gcagctcgct gccccgggag aggcggcggg aacttgtgaa
      301 cggcgctccc cggagggacg tcttcgcggc gttcgactac tatatggagc actacaacct
      361 cgggcggccg ttcatcctcg tcgggagctg ccagggcgcg gaagtactga ttcagctgct
      421 ttccgaatac ataagcaagg attcgccgat ttacggaagg atgcgcgccg catacctcag
      481 cgactgcgac gtccccgagg atttcctgct gtcgaacccg caccttcggc ttgcacggac
      541 gcctgacggg gatggctttg tcatctcctc cgcaggggat gtctccgccg ttccggggag
      601 tgagctgcag aaacagtaac gcggagcact cctctttggc atacaacgcc gcgcccggag
      661 tttccttcag ggcgcggcgt ttacgtttcc ggcagttcga tctcgacggc gagcccgccc
      721 tccggacggt tccgcgcgct gataacgccg ccgtgcagcg ccgccgccct gtgtgcgatg
      781 gcgagtccga gccccgtgcc ttcgcttgcg gatatccgct cgcgggcgcg ctcgacgcgc
      841 cggaagggga tgaaaatagt ctccagctcg ccgggaggaa cgccggggcc ttcgtcttcg
      901 accaggatgt gaagcggccg ctctgtctcc ggaacgcgga agagggagac tcgcaccttc
      961 cctccgtcct gccctgttgt gtgctgcggg gtgtagcgga gcgcgttgcg gatcacgttt
     1021 tcaatcgcgc tccgaagaag cgcctggtct gcgtggagca gcgctccttc ggcgaagtcc
     1081 gtttcgatcc tcttcccgag gggagccgct tcaaaggccg cgtccgcaca aatctgccgc
     1141 aaaagcgccg tcaggtcgat gcgcgccctc cgctccggct gttcgagcgc ttcgaggcgc
     1201 cccagcttca gcagggagcc gatgaggttg tcgatccgtc ccagctccgt ctcaatgcgt
     1261 ttaaaggcct cgtcgtccgg cgacgcggaa cggcggcgca ggagttcgag tcccacgccg
     1321 agccgcgtca acggcgatcg aagctcgtgc gagacgtcgg caagaagctg acgctgcccg
     1381 cgaagcagct cttctatcct gtccgccata cggttgaagt ccttcgccag ctggccgatc
     1441 tcgtcgttcc gcttggctgt atgctccgcc cgcgcgccga gctccccggc ggcgaggcgc
     1501 tgcaccgcgc ctcggagctt cctgaccggt ttggcgaggc ggacggctaa aaaccacgag
     1561 agcaggccgg cgaccagcgc ggccgcaatg aggcgcagca ccgggagact tctgctttca
     1621 aagaaagggt tgagaatgcg tcctgcgaag acgagccccg gaaactcctc cagcgggacg
     1681 gcaaaaagag gacgcccccg ttccatgcgt acaacgggcg cccctgtgtt tcgcgcttcg
     1741 tcgacgatct ttccgatcga ttccggcgcc ttcggctgtc ccgtcagctc cgtaccgtct
     1801 tcgccgaaca gaaacccgaa aaaacggtga gtacgacgga gccgctccag gtagcggacc
     1861 gcttcttccg ggccttcccg cgcttctttc gatgcaacgc gctctccgta gacgagcgcg
     1921 atctcctgta cgttcgcgat catctgctcg cggaagatat ttccctccgt gatcacgctg
     1981 gtcagatggt gcgccagtcc cgtcgccgcc gcggtcagcc agaaccacca gaagagcttt
     2041 aatgtgtagc cgcgcatgca agaaggtacc cttcgcttcg gagcgtccgt atcctggacg
     2101 aaccgtcgtc cgccggtccg agttttcggc gcaggttgct gacgtggacg tccagacttc
     2161 gctcgaacgg tcccagcgtt cgttcaagcg cggccatcgc cagtttttcc cgcgagacga
     2221 ccttcccggc ggagcgcagc aaggcctcca gcagcttgaa ctccactgac gtcagctccg
     2281 tgtctttccc tgcgacgcgt acggttctcg cgccgaggtc catctcgata tcccctgcgc
     2341 tcagacgttc ggaaagcgct gtttccggcc gctcccctct cctgaggaca gcgcgaatcc
     2401 gagcgagcag ctcgcgcgga ttgaacggct tcgggaggta gtcgtcggcg cccagctcca
     2461 gcccgacgat tctgtcgatc tcgtcgccct tggcggtgag catgagcacg ggaacgggag
     2521 agtgtgcgcg cagctcgcgg agcacgtcga acccgttttt ccccggcagc atgacgtcga
     2581 gaagcaccag gtcgaccggc gacgaaagcg cggcggcaag cccctcctcg ccgtcgtgtg
     2641 ctacgagtat ttcaaacccc tccgggcgca ggtattcagt cagcaggtcg cagagttcga
     2701 tgtcgtcgtc gagcagcagt attcgatgca cgttcctttc ctccccgggt gtgcgaagtc
     2761 cgcctcttcg gcggctctct atccgttcat tatacgacga caggctttgg aggggtgtaa
     2821 ggaattgaaa agatgggtat caagagcttt acgtttcttg acctttatcc acaaagcgcg
     2881 aagagcgcat tgagagatcg aggagtacac tcttcgcgtc gggcgaagaa tgccgaaaac
     2941 tcaacgggag gcgaaccacg atgaaaaaga tgaaaaaaag aattttagta gggtctcttc
     3001 tatttgtact ggttgtactg cttgtcggcg gcgtttcaca gtatgcgttc gcaagcagaa
     3061 tgatgggcgc cgggccttgg ggagggatgt cccactggca tgacagggga gattccgcgt
     3121 tcaaagcgga acttctccgg gcgctgaatc cgatggattt caccgaggag cagtggaaga
     3181 agttcgacga gatcctcggc gggctccgcg atcagggaaa gaacttccgc cggggcatga
     3241 tgaaggagat gtggaacact cgcaaagaga ttcacgcgat gatgaccgcg gatggcttcg
     3301 acgaagagaa ggcgaaggaa atgatcgctg cggtccgccc gtcgttcgtg tcgatggccc
     3361 aagaagtgtt aaaggcacgg aacgagctct atggcatatt gaacgacgcg cagcggaaca
     3421 aggtgggcgc tgttctcgac gtcatcggga aacggttgga gcagcctttc agcgggcctc
     3481 gggaactgca tgccgcaatg acgcgccgcc ttgaactgac gcccgaccag aaaggaaagg
     3541 tcgaggcaat tacgaaggag accgcgccca agatgcacgc cttcgcgaag acgctgttcg
     3601 accaggtgaa gtcggagcat gcggcgcttg cgaacggacc gatgagcgac gagcagctcg
     3661 cggaatcggc cggaaaaatc gccgatcttg ccgaaggagc tctgatggag atgggcaaag
     3721 tgagggcgca ggtgtgggag acgttgactc ccgagcagcg cgaagagatg gggaagatgc
     3781 gccatcccat gggcggaatg gagcgcaggc cggggaggat gcggaagtag cagcttcgcg
     3841 gaacgaagtg ctgcgcgcgg cgcgggatct tgatccgcgc cgcgtacaga ctgaaaaaac
     3901 gacgcgcgag aagcgttccg ttcgattttt gaaaggcgtt cgagggagag gttcctcgga
     3961 cgcttttgca tatttccctc gtctccggcg gcgcctctac ctgagtattt gggagatatt
     4021 cctttgtttg acagcgcaca cctccaaaga tacactgtgc taacgaagat atttcaagcg
     4081 ggggcgcgtt atgaaaaaaa actgtgaaca tatcgggagc gccgcggcgt tccctccgtg
     4141 gggaggagcg atacgatgac ctcctccgac ttcgaatacg ggaacgatcc gcaactccgg
     4201 gagtcgatcg agcgcagcat tctcctgggc aatctgcgaa ggatgcagcg ccttctttgg
     4261 gggcttgcgc tctttctggc gctggtgctc ctctgcagtc cttttctgta cgaaaagtcg
     4321 gtgcagcact tcggatccgc agctcccctt gaagtgcaga ttctctggcg cagcgcctgg
     4381 cttgcttttg acctcttcgc gcttttcggt atccgccgca ttctttcgca tccgccggag
     4441 cggatcggtc ttttccacag ggcggtggag acgggcgttc cgctcatcaa catgctcttc
     4501 gtcgtttctc tgctggcagc agggtatgcg tacaacccct ctttggaaat tctgtatatc
     4561 gcccttttcg caatggcgcc tctcatacgg ttgaatgcag ccaaaacatt catcgtcgtc
     4621 gggatgccgc tggggctgtt tgtggcgctc atggaccgcc gcgcggccgg agtcgcgcat
     4681 atgtccaaca tgatcagcct gaccgccgtc gtcattctcg cggcgatcgt cttcagagag
     4741 atgtaccgtt ccaccgtccg cgaaatggag cagcaggcga tcatcgagcg tcagttgaag
     4801 attatggaaa cgtttgcaaa cacggacgga ctgaccgggc tcgcaaatcg caggagcctc
     4861 gacgagaatc tggagagcga atggagaagg ggtatgcgag aacataactt cctggcagtc
     4921 gtcatgctgg acatcgatct tttcaagagc tacaacgatc agtacggaca tccgtccgga
     4981 gacgaatgta tcaggaacat cgcctccgtc atcaaggggt atgcgcggag agcgggggat
     5041 atagccgcgc gctacggcgg cgaggaattc gtgatcgtgc tgccgggcgt cgatctcggg
     5101 gacgccgcgt cgatagcgga gaagattcgc ctggagatat tctccgcaaa ccgtccacat
     5161 gccgggtcgc cggaaggccg ggtgaccgtc agcgcggggg tggcctccgc tccggcggcc
     5221 tctctcgact ccggcgaagc gcttcttcgt tccgcggacc tggcgttgta ccgtgcgaag
     5281 gcggaagggc gcaacagggt ggtcgcggca agcgagagtg cgcgtgttgt tgcggaaaag
     5341 tgaggcatgg agtgtgaaca acgctgatgg aggtgtcgcg tatgtctgga cgtgatgatg
     5401 atcttctggc gaaggagctt gaaaagcttg cggcggcggg cgggaagatc gcggcgaaga
     5461 tatcgggcaa gtcaagtcct gccgtaaccg cggaagattc cgagatcatg cgcaaagcgg
     5521 ctcggcgatt ggataccgat cagtacagag aggagcgtat gatcgagggg gatgcgagga
     5581 gcgtgctgca actcgcgtac gacgccttga aaaacaccgg ccgaatcctg gaggaaaacg
     5641 agcgtgaaag ctccgtgaat ccacggattt ccggtgtggt gggcgcgggt ttcttcggga
     5701 aaaatcctgc ggtggtccac gcggagatag tcggtgcgga tgagaggcgt tgtactctgc
     5761 gcctcaccgg ggcggccaag gaaggactga tcaagcaagg gactgcaaga aaagcagtcg
     5821 agaaggtatt gcgggagttc gactccctga agtcttcgta gaggaatctc ccggaggacg
     5881 cctcccgttc cgtgtacacg gagggtataa tcaagcatca ttctctattt cgggaggttc
     5941 ctatgcgagt gaaacgaaac agtgtccgat acagagcggg catgaagcga acgcgccgca
     6001 tccgtcctct gttgacgtct cttcttccga ccgggtgtct cttttgttgt tcttgtgggc
     6061 gctcggcagc ctgcaggccg tggctcgggg aaggagcggc gtcatgagcg gcatcagaaa
     6121 gtcgatgagt ccgctcgaat ggttgttgct gctctttctt tcggttcttt ggggcggctc
     6181 gttctttttc gtcggcatcg tggtccacgc cctgccgcgc ttcaccatcg tcgcgctccg
     6241 cgtcggcctg gcggcgcttg cgctccatat ccttcttctc ttcaggggag agcgcattcc
     6301 tttggacaga aagctctggg gggccttctt cgtgataggg ctgctgaaca acgtgatccc
     6361 cttcagcctg atcatccagg ggcagatgca catcgccagc ggtcttgcct cgatcctcaa
     6421 cgcgaccacg ccgctcttca ccgtcgtggt cgcgcacttt ttgaccgacg acgagaagat
     6481 gaccgccggg cgttttctcg gcgtgctcgt cggtttctcc ggcgtcgtac tgatgatcgg
     6541 gccggaggcc gtcgccgggc tgggaacgca cgtcgtcgcg cagctctgcg tcctgggggc
     6601 gacgctctgc tacgccttcg cgggcgtgtt cggcaggcgc ttccggggga tgaagctcgc
     6661 cccgatgatc gctgccgccg ggcaggtaac ggcatccagc atcatcctcg taccgctcgc
     6721 cctcgtcctt gaacatccgt ggacgcttcc agcgccggcg cccgaggtgt ggggcgcgat
     6781 tctcgggctt gcgctgctct ccaccgcgtt cgcctacgtc atctacttcc gcatactggc
     6841 ctcggcggga gccaacaacg tgctccttgt gacctttctc atcccggtca gcgccatact
     6901 cctcggaacc accgtcctcg gagaacgtct ggagccgaag cacttcgccg gaatggcgct
     6961 catcggcgtc gccctcgccg cgatcgacgg caggctcttc ggcatgttgc ggcgcttttt
     7021 ccgtacttcc ccgggagaca acccgggaga ttgcgagtga aaaggaggta ccgacatgtt
     7081 ccgacgaaaa atgaaaggtt ctctgcttgt tgcgttgttt ctgctcgcag cgctgcttcc
     7141 gcaggccccc cgcttggagg cagctccgcc gggaacgacg gacccgttcg cccgcgcgcc
     7201 cttcctgctg atggacgagg aggcggtcgt tctgacgcag gagagcggca acatctacag
     7261 cgacgtcttc ctgccgaacg tggtgcagcg cgaggacggc tcccgcgcct ggtccgaacg
     7321 actggacgac gccaagacga cctggtcgcc gagaccgccg cttctggacg gacgggtcgg
     7381 cgacgcggcc gtgctgctgc ggaacagccg gaacgaggcg tttctggcgc aggtctggaa
     7441 tgcaatctct tcgacggggt tgacgatgtc gttggcgcct gtggaccaga atggctacaa
     7501 ggcggagatt atgcgaataa atagtagatg gggttttttt tcaatcaatt atttcgtcgc
     7561 cgccgcgggg gatcttaacg gggatcgggc cacggaactt gtcttggtca gtaccggcga
     7621 tagtaacgga ccgatatttt tcacggcctc cgtcgccgga ttcgacaatc ccgttggaag
     7681 cgagttttcg aacgcaggcg aagtacctcg catacactgg cgctccggat tccgcatcgc
     7741 gaccggcgac gccgacggga acggcaggga cgagatcgcc gtgatgtacg tatcggaggc
     7801 gggaaagtac gtggtggcgc ttttcaaggc ggatgagaac ctgaggctcc gtctcctcgg
     7861 agtgcatacg ctcgacatcg cgccctaccg cgacacatac taccccccgg accagttcga
     7921 catcgccttc gccgactggg aaggcgatgg aaggaagaag atcttctgcg ttacagggta
     7981 cgccggagac gggtacggaa ggaacgtccg cctgctgagc gtgaccatcc aggacggaag
     8041 agcgaccttc accacggaat cgagacactc ccacaagttc ttcggggacc agggccccat
     8101 actggcggcc gcggcggaca tcaacgggaa cgggaaggac gagcttcttc tgaccaatcg
     8161 atggggaagc cccccttcga gcgttcggaa ctcatcgcgg tgtacaacaa gggcgacggc
     8221 tcctccggaa ccgccagtaa gtattggagc aacgatcgac tcgagatcta ccggcccatc
     8281 tcccatctga agacggggcg cttcaccggc tccctccacc cgtcggcggc ggcgaaaggc
     8341 ggatcgcacg cgatggtcct gctccatcag atccattggc gcggggacgg tacgttcgct
     8401 cgtcttcttc gtcttgaggg cgatacgctg aaagaagttt ctgaagctcg tctcagcggc
     8461 ttctcgaatc ccgaccgcgc ccatctcctc gtcggcgacc tcgaccgcga cagcatggtg
     8521 ctgggctacc cgacgcacat cacgttggag aaccacgtca cgccgctcct cttcatgaac
     8581 gaaccgccga agcacatcga cgcggacgga accggagcgg tgacgaacag cacgcgcagg
     8641 gacgacctgt acgtccagta tgcgcaaagc gactcggagt ccagagagac caccaacacc
     8701 ttcgaggcgg agagcaccct cggaggctcg gtgagcgtgg agcaaaaggg agggattgat
     8761 ctcggcatag tgaaagccga gacgaagctg accaccaccg tcggcggctc gacgaaagac
     8821 cttcgctccg agatcgcaag gagctaccag tcgagatcca cgtcgctcac cggggcgacc
     8881 acgagggacg acgtgctgct ctatctctcc aaccgcgtcc acctctggcg ctacccgatc
     8941 ctcggctgga tgagcgaacc ggaaggaggc ggggcgaaag tccccaccta ctatcagctc
     9001 tccatcccgg aagcggaagc gagcatgaaa ttccttcccg ggctgaacgt cgactggtac
     9061 catccggccc acatgaacgg aaacgtcctc tcctaccctt cgagaagagc acagataggc
     9121 gatttcgccg aagacaggcg actctccgac aacgtcgagt tcggcgtcgg cggcggaacg
     9181 gcgttcacaa gaggcatcac gtggaaggac tcgtttacga gggagagcct gaagtcggtc
     9241 gaggtcaaaa tgaacctcga cgtggaactg caggtgagcg gcagcgcgca ggtcgagatc
     9301 ttcgaggtcg gcggctcgac gaccgcctcc ttccacatgg acaagcggac gaccgagacg
     9361 agcgtggcga agacctccgt ggagaaggag aaggcgttca ctgtgaacat cccggcgttc
     9421 tcgacgccgc tcggctatca gatcacgccg ctgatgtaca gaacgggcgg cggcgtcctc
     9481 aaaacatccc acgtcgtgga cgtgctcaac aaccggtcgc tgtgggcgag ccgctacgcg
     9541 gcccccgatc cggccctcaa ccttccgcgc ctctggaggt acgtcgaagg cgacggatgg
     9601 aagtgggcgg ccgccgaccc gctcaacccg gacgcgcgga agatacgcgg catcttcttc
     9661 cggaacgcga acggcgtcga cctgggaaag agcctcccgc tgggagaaac aacgacgatc
     9721 gccgtgcgcg tgcacaactt cagcctggtc gagtgccccg aggtggacgt cctcttcgag
     9781 gccgttccct acggcggcgg cccggcggtc gttatcggaa ccgcacggac gccccggata
     9841 gacccgtggg gcgcttccga agcggccaac tggaagtggg cgacagttgc gtggaacacc
     9901 tcagggatga cggaggggaa ctacacggta cgcgtcacgg cgaaccccga cgggcgggtt
     9961 gcggagcttg agggacgggc cctgaacgag ccgttcgaca acaacctcgg ctggtacgac
    10021 gtcttcgtgg gcaggtcgcg gagcgccgtc gaagaggaac ttgccgctgt gaacgagaac
    10081 ctcgtcgccg cgacgctcga actggcgaac acccccgccg acggcaaggc cttcgctctt
    10141 ggagaagtcg cgctgcttcg cggaacggtg cggaacgaag gcgtgctgcc ggtcggcaac
    10201 atctcgctct tcttctacga cggcaacccc gacggaggag gcaagcccat cgccaacaga
    10261 atcgtctccg gaatcgtgcc gggcggcgag tactccgtcg tgctgacgca ctccttcgac
    10321 acgccggggc cgcgcagcgt cttcatgagg gtggtcccca aggttggcga cccccccgag
    10381 gacaacacgg tgagcgtggc cgtcaccgtg cagggcggcg actcgggcgg cggcggatgc
    10441 gccgtcggaa ctgcgggcgc atggagcgcg ctgctcctga taggactggc gccgctggcg
    10501 ttcggaagaa aggagaggta aggcatgaag aatcttggtc tcatcccgaa actgatcatc
    10561 ggcatcatcg tcggcattct cataggtaag ttcggcgggg ttcttcccgt gcggattctg
    10621 gcaaccttca acggtctttt cggcaacttc ctcggttttg tcattccgct catcatcatc
    10681 ggcttcgttg ctcccggcat cggcgagctg gaggccggcg cagggaagct gctcggcatg
    10741 acggctggca tcgcctattc ctcgaccgtc atggcggggc tcgccgcctt cttcgtgaac
    10801 agcgcgctct tcccgctctt tctgacgccg gggagcatcg tgctcgacga tccggggaat
    10861 ccggagcacg cgctgctccc ctcgctcttc aacgtcgaaa tgccgccgct gatgggcgtg
    10921 atgacggcgc tgctgctggc gttcaccctc ggcctgggga tggcggccat acgcagcggc
    10981 gcgctccagc gtgggttcaa ggagtttcag cagatcatcg agaagctcat caaggggatc
    11041 atcattccgc tgctcccctt ccacatcgcc ggtatcttcg ccaacatgac gcacgccggg
    11101 caggtggcgg agatcctctc ggtcttcctg aaggtgtttt tgatcatcat cgcgcttcat
    11161 ttgggtgttc tggtattgca gtacgtaacg gccggaatcg tcggcgggaa gaatcccgtt
    11221 cggctgctgc ggacgatgat tcccgcctat ctgacggcct tgggaacgca gtcttcggcg
    11281 gcgaccattc ctgtaacgct cgagcagagc gtccgcaacg gagtccgcag aggcgtggcg
    11341 aacttcgtcg tcccgctctg cgccaccatt cacatgtcgg ggagcaccat caccatcacg
    11401 agctgcgcga tggcgatcat ggtgctcaac ggcatctcct tctccttctc gacgatgctc
    11461 ggcttcatca tgatgctcgg cgttacaatg gtggccgctc ccggagtgcc gggcggcgcc
    11521 gtgatggctg ctctggggct gctgcagacg atgctgggtt tcgaccagac gcttatctcg
    11581 ctgatgatcg cgctctacgt ggcgcaggac agcttcggga cggcatgcaa cgtaacgggc
    11641 gatggcgcga tcgcggtggt ggtcgacagg atttacggaa agctcgcgga ggacgaaccg
    11701 tcgggcgcag cctcctgatc cggtaaccgt tcccacctct tcagaaagaa cgaacgggcg
    11761 cgtccaccga tgcgcccgtt cgtcctttct ccgtcggttc gtggtttgac cgcgacaatc
    11821 atgtcgtcag ccagcaggtt ccatagggat tcagcacgac ctccgcgacg ccgcgctccg
    11881 tcctgtactc ctcgttcgtg agcagatcgc ggcaacttcc gtcgaggccg ggaaggtgtc
    11941 cggcgctgat cgtgacgtgc atcgggctta ctgtgacgtt ttggaggcag agcaccgggc
    12001 gccccccctc tggaggtgtt cggagcagcg cgaacagccc ctcgccgata tcgagcacct
    12061 tctgctcgcc ttccgggtga aaggcctgct cctcgcgtcg gacgcggagc aggcggcgca
    12121 gttcgttgaa gatccgtccc ggccgcccag cgggatcggc gattgcctct tccagccccg
    12181 agcggtcgaa cttcttgcgg ttcagggagc gcgcccgtcc ggtctcggcg aagcattcat
    12241 ggcagttgcg cgagccgaag aggctgtgga cgtagatgcc cggaactcca gcgagcgaga
    12301 gcaggattgc ctgagaggcg ataaaacgcg ctgcgtccgt ccccggctcc ggtttcgccg
    12361 gatcgttcag gaagtcgtag agggtgatgt tcagctcgta cggactgatg gagccgtcgg
    12421 cgttcgtctt gtacgagacg tgcccgccgt gagcgagcgc gcgctccgcg agtctccgca
    12481 cctgttcgtc gctcaggatg cccctggccg gcgtcactcc gatgccgtcg tgcgaggcga
    12541 taaagttgaa gaaggtcgct ccgcgtgccg ggggggaaag ctccgaggcc cacgtcgaga
    12601 ggacggttgc gtctcctgtg agcagggcgt gaagcgtcag cggcgccagc gaaaagttgt
    12661 acaccatctg cgcttcgtcc gttcctcgcc cgtcttcccg cgcctcgccg aagtagctga
    12721 cgttttcggc gtgagggacg tttgtctcgg tgatcagcag cacgtgcggg gcgacgtcgt
    12781 cgagcacggc gcgccagaat ctgactactg catgcgtttt cggcaggtgg atgcaggatg
    12841 ttcccggcgt cttccagaga taggcgatgg cgtccagccg tatgatctcc gcgcccctga
    12901 atgcgtagtg gagcaggatg ccggtcatct ccagcagcac gcgcggcgcg gcgtagttca
    12961 gatcgatctg gtcggcgctg aaggtcgtcc agacgctccg ttcgccttgg gccgtctcaa
    13021 cctttgtcag cagcggcagc gtgcgcggcc gcaccacccg tgagatgtcg gcgtcttcgg
    13081 ggggggtgat gaaatagtcc gtgtagggcg cctccccgcg gagaaaggag gcgaaccagt
    13141 cgctccgccg tgagatgtgg ttggcgacga agtccagcat caggcggtat cgctctccca
    13201 gcgcggcgat gtcgtcccag tcacccagct ccgggtccac gcgccggtag tcgacgactg
    13261 aaaagccgtc gtcggaggtg taggggaagc aggggaggat gtgcacaccg ctgacgcacc
    13321 cttcgaggtg tgtgtccagg aagccccgca gcgttcggag ggggggctct ccgggcgcgc
    13381 ggaactggtc gccgtaggtg atcaggatgg cgtcccgttc gtccggtaga tactctcttc
    13441 gcgagggggc cggtattttc cctcggtacg aatccatcag cgccttcagc tccggcagga
    13501 cgcgctccgc ctcctcctct ccgtagagaa agatcagcag cctcttgatg tgtgcgctcg
    13561 tgtcgttcat tagcgtgctc ctttgagaat gggtgtcaaa aaacggatgg cgtacgcggg
    13621 gcgggatcaa agctgctcgt acagcacgtc gatcagcagc gccgcagtcc atgagaagcg
    13681 gtgcgcgccg tgcccctctc ccgtttcagg gttgaagtac tcgcgaaagc ccccctttgc
    13741 cgggagttcc gcgattgtgc gccggagccg ttctgcctga tcgttgcggc cgtacgccct
    13801 cagaccgtgc atcaacagcc agttcatctg gatccacgtc gggccgcgcc agtagcgcct
    13861 cggggagtat tccggcgttt cccggtcgta gctggacgcg ggatacccct tctgctccgc
    13921 tccaaggccg aacgaggggg tgagcagacg gcgcagcatc gtctccgcgc gctcctcacc
    13981 ggggatgcgg gcgaagaggg gggaaaaccc ggccagcgcg ggcatcggta tcggcttttc
    14041 cgaggcgaga tcgtagtcgg cgtagtagcc cctgtcgccg tcccacagct tcctgttcac
    14101 ggcgtcggcg gtgcggcgag cgcgcgcttc gaacggcgcg gggttttcgc ccagaagacg
    14161 ggccagctcc gccatgtcct gttccgcgcg gcagagcagc gtgttgaaca ggacgtcctg
    14221 caccagaaac gggcagccgt cgccgaagat cttccgttcg tcgtaccccc gctgccggaa
    14281 gaagtccaca agaaagatat agcggtcgta gtccgagtcg agcggccgtt cctctggagc
    14341 tacggcgcga gtgtctcttc tgcggaagcc ggaaaaatgt gcgggcggca cgtccatccg
    14401 cgccagcacg ggatcccaca ggggggagtt atcctggccg gactcccacg ggtggatgat
    14461 gtacaccaca ccctcgcctc tcgggtcgcg ttcccggtag agccagtcgt gccatgcgcg
    14521 cagcttgggg aacatttctc ctgcgaaggc gagcgccgcc ttctccccct cctctcccgc
    14581 gcagcggacg atgtggagga ctgccgtggc gtggttcggc ggctgtacga tgccggaagt
    14641 gcgtggggaa agaggggcga acgggtttcg ttcggtctgc cagaattccg gacccgggaa
    14701 gtacgatgcg tcggggctcg cgttgtggaa gacgatatgc ggtacgcgcc cgtccgccca
    14761 ctgcgcgctg aagagagaac gaagttcgcg ctgcgctcgc tcccggtcgt agcgggcgta
    14821 cccggcggcg atgaacgcgg agtcccagga ccactggtac gggtactggt tctgcgcggg
    14881 aacggtgaat ccgtccttcc agttttcgtc gagcacctcg cgagccgatt gcagcagcgc
    14941 ggttgtctcc tgtgaagcgt tcattgtaca gcctcccttg ccttcataag cgctcccgga
    15001 cgaagccggc cggaatcgtg cgaaacggtg tccgcgagag cggcatgttc gtttcactct
    15061 tcgcctcgct gcggaaagtt cctcagagtg acggatagag cgcgcgcttc gtgtccggat
    15121 cgatccagcg gacgctctcc ggagggaagc gaagccagac ttcgcctccg gcgcgcgccg
    15181 gaaatccggg gtgcgtggaa accttgattc tatggccgcc gaaggccgca gtcaacagat
    15241 tctgcgagcc gagcggctcc acgaccagaa cgtcggtccg cagggcgtca tgggccggtt
    15301 cgtgcagcgt ctccagattc tcggccctga tgcccagcac gatgggcttg ccgtcgtact
    15361 ccttcaggac cggctgcatt cggggcgttg gcgtcacgtc gaactctccc aggcggacaa
    15421 aggtatcgcc gtccttcctg cggacctgcc cttcgagaaa gttcatcggc gggttcccga
    15481 tgaatcctcc gataaagcgg ttcgcagggt agtcgtacac ggtcgtcggg gagtccacct
    15541 gctggaccgt tccgccgtgc atcaccgcga tgcggtcgcc catgctcatt gcctctatct
    15601 ggtcgtgcgt gacgtagacg gtggtcgttc cgatctcggc cagaagctgt ttcagctcgg
    15661 cgcgcatctc cagccggagc agcgcgtcga gattgctcaa cggctcgtcc atcagcagca
    15721 cctgcgcctg catcgcgatg gcgcgcgcca ccgccacgcg ctgcctctga ccgccgctca
    15781 tcttcgacgg gtagcggtcg agcatcgcct cgatgtgcag cagctccgcc gcccgcctca
    15841 cccggttttc tatctccgtc ttctcctcct tctgcatccg caggccaaag gcgatattgt
    15901 cgtagacgtt catatgcggg aagatggcgt agctctgaaa gaccatggag atgcgccgtt
    15961 cccgcggggg caggtaggtg acgtcccgcc cgccgatgct gatcctgcct tcgtccgcca
    16021 tgccgagccc ggcgatggcg cggagcagcg tcgtcttgcc gcagccgctg ggacccagca
    16081 gcaccatgaa ctcatgctcg gcgacgacga gcgagacgtc gttcaccgcc acgaagtcgc
    16141 cgaacctctt gacgacattt tctattcgta tctccgacat gattccgctc cttccgtaat
    16201 tttgttcact tgctggactg cccccacatg ttgaaaaggt acttgcggat gatcatgatg
    16261 aagatcaccg agggaatcaa catgaagaat cctcccgcga acttgtacgc gtcgggggcc
    16321 tcccgcagcg agacgagcac ctgcgcgggc agcgtgcgat tctgcaccgt gagcactgtg
    16381 gccgcgaaga cttcgttcca ggacatcacg aaggtgaaga gcgcggcggc ggcgatgccg
    16441 ggcagcacca gcggcagcac gacccgccgg aatgcctgga aggccgtgca gccgaagacc
    16501 tgtgccgcct cctcgagttc atacggaatg ctcgcgaaga cgctcccgat gacgagcacc
    16561 gtggtaggca gcgtgagcgc cgtgtgcatc agcgcaagac ttgagacctt gtcgaacatt
    16621 cccaggttga tgaaggtcac cgcaagcgga atggagagga tgaccaccgg aaaggcgcgc
    16681 acggcgagga tggagaggcg gaatgcgtta cggcctcgga agacgtagcg cgagagcgcg
    16741 tatcctgccg gcgccgcgat cagcgtggag agggccaggg tgatcagcgc gacgacgacg
    16801 ctgttgataa gaccggggat gataccgcgc gcctccagga agaagcgcat ggtgtccaag
    16861 gagaacggaa tgaagggcag cacgcccttg ggaaagtttc gcatcgcttc cggcgtggtg
    16921 agggccatcg acgcgatgag ccaaatcgga agaagaatcc acagagagag aacaatcgcc
    16981 gtcagatagg tgagcgcccg cttgatcacg aggcggcgct tcgcgcgagc gcgaaccttc
    17041 gtcatctcgt ccgaaacccg tacgctcatg agaccgcctc ctcctgtccg cgtacagtac
    17101 gcagatagat accggcgctg atcatcgaaa gcaggagaat caggccagaa tatgcggcgg
    17161 ccacgttcat gttgcggagg gcgtagtact ggcggtaggt ctcgttggcg agcaccgtga
    17221 ccacgtcgcc tccgccgagc gcgatcacca cggcgaagac ctgcagcgcg aggatggttc
    17281 tcaggatgag ggcgacctga aggcttggct tgagcagcgg aagaatcacg tagcgcaccc
    17341 gctgccacag ggtcgcgccg aagagttcgg ctgcctccag cacctcctgc gagatcgcct
    17401 gcagcccgga gacgacgatc accatcacga gggacgtcgc ccgccagacc tcggcgatcc
    17461 agaccgcgac caggatccag tggcgcgtcg cggcggtgag gtatgcctgc gggctttcga
    17521 tcagcccgag ggagtagaga atgctgttca gatagccgtt ctgcgtgaag atggaggacc
    17581 agacgatccc cacggcaagg tccgacgcgg ccagcgggat ggcgaagatg gcgagaaaga
    17641 gcagattgaa cttcagccgc gcctgaatca ccagcgccat cacgatcgcc agcacgagct
    17701 gcacaggaat gatcagcagc atcaacagga aggttgttcc cagcgcggag cggaagaagc
    17761 ggtcgttgag catcctctga atgtggagca gggtgaagcg gccggtatcg ccggagtcca
    17821 cccttccggt cgcgccgtcg ccgtagatct gcagcctgcg ggagggcgcc catccggaga
    17881 gcgtctgttc attgcgtact ccttcaataa gaaaccagac ttcaagagat gtccgttgcg
    17941 caata
//
LOCUS       DAOPKU010000003        16342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_3753_length_16342_cov_4.911709, whole genome shotgun sequence.
ACCESSION   DAOPKU010000003 DAOPKU010000000
VERSION     DAOPKU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 16342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16342
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3753_length_16342_cov_4.911709"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PK849_00160"
     CDS             <1..381
                     /locus_tag="PK849_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPE64561.1"
                     /translation="VQMATRFACTYEGDASDRFKQAYIDATEEDVVLIESPAGLPGRA
                     IRNPFVNDYLEGHVKSKPCIANCLTHCRYKKTRETFCIAQALVDAFNGNWEEGLFFCG
                     SNVVKVKEMQHVEDIMRDFFPEMA"
     gene            474..2816
                     /locus_tag="PK849_00165"
     CDS             474..2816
                     /locus_tag="PK849_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPE64562.1"
                     /translation="MKTTLRRCVVICAMLLAELFFCILIFGSAGSANLAEASQEAEKT
                     VLEVAKVNVEASVREIPLDKQAIQDRITEIQTELDALIEKKESLLETENIEEDDLRRT
                     LVALGSLQNVYSRLTSAQDNLTEAKREQEAQEKKSAEPVISGQPPFNLSFYEDVRNRY
                     ESVEQRLEAVNSSLRLSEGSVSTVDEQIRRMEEQHRVLSEELQNLKNASGVKQLLQVK
                     ESEADLETAKASRIVQDLNIEAQNISRNLLLAERDKLKTGLEFVKKHVAYDEKDRTAR
                     AERIGEQIAAAREKLPGLREEREKARTALSRSQGALTSARNERERVLAQAGVAEQQAA
                     LQLAQVSLEHTELTLLFLEEEQRIWNARYSLLNGSLKGQELWDMRSLIVTRVKDLESA
                     FLVRQRDVSSIQAEMHAAQKELEEHTGANAVLARIRARIDLLNRIAESANASMARIFS
                     LFNQYNRLREEINDHIDAVKIAERVTTFGKERFLAFWNITLWSGDGFDITVAKLVFAV
                     SLFLAAFFLSGRLTDFLSRTLLNRFGLDSTGMMASRKILFFILMGALILAALDLVGIP
                     LTAFAFLGGALAIGIGFGAQNIFNNLISGFILMFSEPMRVDDTIEIDGLFATVQEIGT
                     RATRVKTFDNIDVLMPNSYFLNNKIVNWTLTDQKIRLRINVGVVYGSDARKVEELLLL
                     AAHDHTRVLRNPEPFVVFREFGPKALEFALYFWIDMTNASTLKVSSDLRYRLIALFEK
                     NGIKIAFPQMDVHLDTLSPVDICLNRPRTADTEQTHTQEE"
     gene            2823..4460
                     /locus_tag="PK849_00170"
     CDS             2823..4460
                     /locus_tag="PK849_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE64563.1"
                     /translation="MLSLAENDLYSLLLDLVAIPSVSFSEEENRAADFIFSTLSDLEY
                     FRRNPSFLRFLPAEGDPLGRKAVAALVKANPRTKKTVIITGHFDVVDAEACGHLKNLA
                     FSPEEYTRRVDELEIPEEARDDLASGEYLFGRGVSDMKSGIALEMCLIGALSRLESFP
                     ANVMFLAVPDEENTSAGMRGAVPWLVRLRNEWELEYTACLNSEPSVGGRGIPAGGVYV
                     GTIGKIMPFYLCVGKEAHVGDYYEGVSASLLTSYLSLILEGSPETAESLDGVHFPPQA
                     CLRFKDLVKNYSVTLPERSVAYYNCLTVSRTPARILEEMKTAAGRALEMTLTHLSKAR
                     QTLRERGASLPEKKAFPPRVLSYGDLLEKVRSRTERFDDVLESFLKTLPDSMDERDLC
                     IETASFLLDLSGEKGPLILVGFLPPFYPPRLNRSESAGERALLRAVERLKEAGKPYGL
                     SISRIDVFQGIMDLSYLGFQGAPSELDALAENMPLWGRGYRFPLNELKKLDVPIANFG
                     PIGKDDHKNAERIHLPYYLHTLPPLFFKFVEFLAEES"
     gene            4748..5974
                     /gene="rocD"
                     /locus_tag="PK849_00175"
     CDS             4748..5974
                     /gene="rocD"
                     /locus_tag="PK849_00175"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPE64564.1"
                     /translation="MSRNRSEELMEMEHEYGAHNYHPLEVVISKAEGIWVEDPEGRRY
                     MDMLSAYSAVNQGHRHPKIIKALKDQADILTLTSRAFFNDRWPVFAKKLADFTGKEMI
                     LPMNTGAEAVETALKTMRKWGYMVKGVEKDKAEIIVFENNFHGRTITIISFSIDPDAT
                     DYYGPFTPGFKVVPYDDLEAVEKAVTKNTVGIMVEPIQGEAGVVVPSDGFLKGLETIA
                     RKHNVLLAIDEIQTGFCRTGKNFAFQHEGIDPDIIIMGKALGGGVFPVSAIAANKDVL
                     GVFKPGTHGSTFGGNPLACAVASAAIDVLVDEKMVERAAEMGKYFMDGIKAIQSPKIK
                     LVRGKGLLIGVVLNESAGKARLYTTALKEKGLLCKETHDWIIRFAPPLVITKQEIDQA
                     LKTIQEVFASTPIPCV"
     gene            6260..7531
                     /locus_tag="PK849_00180"
     CDS             6260..7531
                     /locus_tag="PK849_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE64565.1"
                     /translation="MKRNWMIALVVLVVVAGYFFMQQQAPQPPKEEPVKESPTPTPEA
                     PKAEEPKAEEPKAEEPKASEETVKIGYLAALTGDWAAYGQTEEKTARLAVDEINAKGG
                     VLGKKLELVVYDFRTRAEDAVNAVRRMIEEDKVVAIVGANGSGINIATAPIVNRLGVS
                     QIGTVSTNPLVTVDEKGEVRPYSFRICFTDPYQGKLIAFFAAKELKRMKAAILYDVGN
                     DYSQGLREFFMKSYAEYQGEVVADLGFRGGTDVDFRAQLTEIRDKGADVLVLPNMGKE
                     MALIMKQARELGMNEIVFVGGDGYGEFMWEIAGEAMEGSYWINHVAPEDPAMAPFFAA
                     YKEKWDDECKEFVNGVLGYDSIYWVADAISRAGKADPKLIRDALEATKDLKLHHATIT
                     MDPKTHNPVDKDGVVLIAKDGKGQFFTKIKP"
     gene            7655..8536
                     /locus_tag="PK849_00185"
     CDS             7655..8536
                     /locus_tag="PK849_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE64566.1"
                     /translation="MATLIQQIINGLSLGSVYALIAVGYSLVYSVLLFSNFAHGGFLV
                     IGGYICYAALRTFNIGIWMAGALSLVGAGAAAILTERLAYRPIRERTNVTLYLLIASM
                     GMSIVIENIFVIVAGGRFRALPPVIPTTPVNFFGLATTSAFDLLSLVSAVVFLGALQL
                     FLSKTRWGLAIRAAAYDLRTAGLMGVNVNRLISIVFFVAGALAAVGGIFLSVRYTLYP
                     QLGAITIKAFVAAVIGGLGSLPGAVVGSLILGLAEMLTAGFISSQMRDLVVFSLLVVT
                     LLVKPTGLFGKSVSEKV"
     gene            8550..9401
                     /locus_tag="PK849_00190"
     CDS             8550..9401
                     /locus_tag="PK849_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE64567.1"
                     /translation="MSGYAEGIIILLCINAIAAMGVSLLTGFTGIFSLGHAAYMALGA
                     YTSAILTVQHGVHFFPAILLGGVVAVALAYFIGVPTLRLMGDYYAIASIGLGEAIRLI
                     LENWQSVTRGARGYPGIDPYTSLPIAVGFFVILAIFMFNLINSRFGRACKACRDDYIA
                     SSLLGFNTARIRVVSLMISAFYCGVAGALLGGFLSFIQPVMFDMLKSTELTAVVVFGG
                     LGSMSGCILGTAVITLVTELFRPISQYRMLIYGAVLVIIMVLRPEGIMGNRELLSWIK
                     DPFGRRS"
     gene            9401..10174
                     /locus_tag="PK849_00195"
     CDS             9401..10174
                     /locus_tag="PK849_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64568.1"
                     /translation="MSAIFELSGVTKNFGGVRAVSEMSFSIPEGELAGLMGPNGAGKT
                     TIFNLVTGVYDPDEGSILFRGENLVPLKTFDIVHKGIARTFQNLRLFQRSSVLENVMT
                     AAQQHVPYTFFEGMTHLGRWRSKESDIRKKSMELLERVGLADRANQAAGTLPYGHQRR
                     LEIARALALDPKLLLLDEPAAGMNAEEVLSLNSLIRSIHTDFKLTILVIEHHMDLIME
                     ICPHIVCMNFGAKIAEGTPDEIQNHPEVLKAYLGEEEEE"
     gene            10171..10941
                     /locus_tag="PK849_00200"
     CDS             10171..10941
                     /locus_tag="PK849_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64569.1"
                     /translation="MSDAVMNEKKALLEVHDLHVSYGAIKALSGVSLKVFEGEIVSVI
                     GSNGAGKSTLMNAIMGMVRREKGEILLGDVKLDSRSFRVVQQGISLSPEGRKVFAPLT
                     VLENLKMGAFPRGNDSKVEEDYEWVFSLFPRLKERISQYAGTLSGGEQQMLAIGRALM
                     SRPKVLLLDEPSLGLAPIIIKDIFKELRRINNEGVTILLVEQNARQALLLSNRGYVIQ
                     TGRLVTEGPSEELLANADIKAAYLGGGHAKGKTTQALA"
     gene            11072..11533
                     /locus_tag="PK849_00205"
     CDS             11072..11533
                     /locus_tag="PK849_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPE64570.1"
                     /translation="MAIKLSTKTRYGLRALLYMAERYDGERTVPVGEIAKEQEVSEKY
                     LEQLFLKLRRSNLIQSVRGAQGGYMLGRPPEDITIACVVDSLEGDITFADCLSPGGCR
                     NQGSCPTHELWSRLKGSIDEILEDTTLADLLKNSATNHAGEKSGKGSCSSK"
     gene            11550..12743
                     /gene="nifS"
                     /locus_tag="PK849_00210"
     CDS             11550..12743
                     /gene="nifS"
                     /locus_tag="PK849_00210"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HPE64571.1"
                     /translation="MSRQVYLDYAATTFTAPEVVSAMTPYFTQSFGNPSSIYSFSESN
                     RKAMGIARQQVADVLGAAKEEIFFTSGGTEADNWALKGVAWRNRSKGNHVITTKIEHH
                     AVLHTAQFLQENGFEVTYLDVDAEGRVRLEDLKNAVTEKTILVSVMFANNEIGTIQPI
                     AEIGAFCREKGIIFHTDAVQAVAHVPIDVKAMNIDLLSLSAHKFYGPKGVGALYVRKG
                     IKIDNFMHGGGQERGKRATTENVAGIVGLGAAIELTASRMKDASSRVGKLRNMLIDGV
                     MKVIPHAKLNGAPVSERLPNNSNFSFIGIEGETLLLDLDGAGIYASTGSACSSGSLDP
                     SHVLMAIGLSHEQAHGSLRMTLGEETSEEDIRYVLSVLPAIVERRRAMSPLWEDFLKN
                     TQGGK"
     gene            12748..13173
                     /gene="nifU"
                     /locus_tag="PK849_00215"
     CDS             12748..13173
                     /gene="nifU"
                     /locus_tag="PK849_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HPE64572.1"
                     /translation="MYSEKVLDHFQNPRNVGEIENPDGVGQAGNPKCGDIMKIYLRVE
                     NEVITDIKFKTFGCASAIASSSMATELVKGRNIDEAWNLTNSAVAEALDGLPPVKMHC
                     SVLAEEAIHAAINDYRRRKGLPVLEGKEYDHEHDHAHQH"
     gene            13177..13752
                     /locus_tag="PK849_00220"
     CDS             13177..13752
                     /locus_tag="PK849_00220"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPE64573.1"
                     /translation="MNGAFNRCPWPGTDPLYVAYHDEEWGVPVREDATLFEFLLLEGA
                     QAGLSWITVLRKREAYRSAFDGFDPEIIAGYDERKIGELMRNEGIIRNKRKIEGAVKN
                     ARAFLRIRDRKGSFSAYLWDFLDGEPIVNRWKTMEEVPAVTPLAEKVSGCMKKEGFVF
                     FGPVICYAHMQATGMVNDHLTSCFRHPESGY"
     gene            complement(13813..14019)
                     /locus_tag="PK849_00225"
     CDS             complement(13813..14019)
                     /locus_tag="PK849_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64574.1"
                     /translation="MAGKYGFTAATETEREVFRRNDRHLDDMFSVSWRGPFEGFSASR
                     KADLAEPSFSQTEMRWRGPFEGLR"
     gene            14485..15702
                     /locus_tag="PK849_00230"
     CDS             14485..15702
                     /locus_tag="PK849_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPE64575.1"
                     /translation="MVNGFFVFFVFAAVYLGMIFGRFPRLALDRTGIVLLGAIVLLAA
                     GKASLEGGRSGIDVPTILLLFGFMVISAQFRLGGFYSEVSRRIGALAAPPSLIMGAVI
                     GASALMSALLTNDVVCLAMTPVLGAGCIRRGLNPLPFFLALACAANIGSALTLIGNPQ
                     NMLIGQYLSLPFGGYLLFAAFPVFLSLLSLWGIMLFLFRGRWTLQMDEQLVDERSFDA
                     WQTLKGGVVLTVVVGLFLFSTLPRDLTALGAAGVLLLSRKMASRRTLEIVDWQLLLLF
                     LGLFVVNAAFESAGGLQILKEFLESAGVSLSSPAWLYGVTTVLSNLVSNVPAVMLLLP
                     LVRDVPQAGYILAVSSTFAGNLLIVGSIANIIVAGEAERMGIPFDWKQHALVGVPVTL
                     ISLAAGLACLWLF"
     gene            15834..>16342
                     /locus_tag="PK849_00235"
     CDS             15834..>16342
                     /locus_tag="PK849_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPE64576.1"
                     /translation="MDLSILFRGVVIGFSIAAPVGPIGVLCIRRTLTEGRASGLVSGL
                     GAASADAVYGCIAGFGLTFLSNFLVSRQTELRLAGGLFLLYLAVKTFFAQPAQKAAEV
                     RGHGLLGAYLSTLFLTLTNPMTIISFAAIFAGLGLAEGSGNYISAGVLVLGVFAGSAL
                     WWLLLSGGFG"
BASE COUNT         3647 a         4291 c         4630 g         3774 t
ORIGIN      
        1 gtccagatgg caacccgttt tgcctgtacc tatgagggag atgcaagtga ccgtttcaaa
       61 caggcctaca ttgacgcaac agaggaagac gtggtgttga tagagagccc tgccgggctt
      121 cccgggaggg ctataaggaa tcccttcgtc aatgactacc ttgaaggcca cgtgaaaagc
      181 aagccctgta tcgccaattg tctgacccac tgtcgataca agaaaacaag agagaccttc
      241 tgtatcgccc aggcccttgt ggatgccttc aacggcaact gggaggaagg gctcttcttc
      301 tgcggatcaa acgtcgtgaa ggtaaaggaa atgcagcacg tcgaagacat aatgagagac
      361 ttctttcccg agatggcata attcggactc gccggagcgt ttctgccgcg cttttccttt
      421 tcaaggcggg agcttgctcc cgccttttct gatagaaaga ggaggagaac acgatgaaga
      481 ctaccctgcg tcgttgcgtt gtgatctgcg caatgctctt ggcagaactg tttttctgca
      541 tcctgatttt tgggagcgca ggctctgcga accttgccga agcatcgcag gaagcggaaa
      601 aaacggttct ggaggttgcg aaggtcaacg tggaggcctc cgttcgtgaa attccattgg
      661 acaagcaggc tatacaggat cgcatcactg aaattcagac ggagttggac gctctcattg
      721 agaagaaaga gagcctcttg gagactgaga atattgaaga ggatgatctg agaaggacgc
      781 ttgtcgctct cggctctctt caaaacgtct attccaggct gacgagcgcc caggataatc
      841 ttacggaagc gaagcgggag caagaggcgc aggagaagaa gtcagcggag cccgtcatta
      901 gcggacaacc gcccttcaac ctctcattct acgaagacgt ccgtaaccgg tacgagtctg
      961 ttgagcagcg tcttgaagcc gtgaattctt ctctccgtct ttccgaagga agcgtttcaa
     1021 cagtagatga gcagatacgg aggatggaag aacagcacag agtcctttcc gaagaattgc
     1081 agaacctgaa aaatgcttcc ggcgtgaagc aattgctcca ggtaaaggaa tccgaggctg
     1141 atcttgagac cgctaaggcg tcccggatag tccaggattt gaacattgaa gcgcaaaaca
     1201 ttagccgcaa tctgcttctc gctgaacgcg acaaactgaa aacaggattg gagttcgtta
     1261 agaaacatgt agcgtacgac gagaaagacc ggacggcgcg ggccgagcgc atcggcgagc
     1321 agatagcggc ggccagagag aagctccccg ggctgcgcga agagagagag aaggcgagaa
     1381 ccgccctctc aaggagccag ggagcgctga cttcagcaag aaacgagagg gaaagggtgc
     1441 ttgcgcaggc tggagtagcc gagcagcagg ctgcgcttca actcgcgcag gtttctctgg
     1501 aacatacgga actgacgctg ctctttctcg aagaggagca gcggatatgg aatgccaggt
     1561 attctctcct caacggaagt ctcaaggggc aggaactttg ggatatgcgc tcgctcatag
     1621 tcacacgcgt gaaggatttg gagagcgctt tcctagttcg tcaaagggat gtttcctcca
     1681 ttcaggcgga aatgcacgcg gcgcaaaaag aactggagga acacactgga gcaaatgccg
     1741 ttcttgcaag aatacgtgcg aggattgatc tgctcaaccg catagccgag agcgcgaacg
     1801 cctcgatggc ccggattttt tcgcttttta atcagtacaa ccgactccgt gaggagatca
     1861 acgatcatat cgacgcggtg aagattgcgg aacgagttac caccttcggc aaggagcgtt
     1921 ttttggcgtt ttggaacatc actctctggt ccggagacgg gttcgacatc acagtggcaa
     1981 aactggtttt cgcggtctcc ctcttcctcg ctgcgttctt tctgagcgga cgtctgaccg
     2041 atttcctcag caggacgttg cttaaccgtt tcggtctgga ttcaaccggg atgatggcca
     2101 gcaggaaaat tctctttttc atcctgatgg gcgcccttat tctggccgcg ctcgatcttg
     2161 tcggcatacc gcttacggcg ttcgcgttcc tcggaggcgc ccttgccatc ggcatcggtt
     2221 tcggcgccca gaatattttc aacaacctga tcagtggttt tattcttatg ttcagcgaac
     2281 cgatgcgagt ggacgatacg atcgagatag acggtctttt cgcgacggtg caggagatag
     2341 gcacccgcgc aacgcgagta aaaacgttcg acaacatcga tgtgctcatg cccaatagct
     2401 attttcttaa taataaaatt gtcaactgga ccctcactga ccagaagatt cgcctgagaa
     2461 tcaacgtagg cgttgtgtac ggttccgacg cccgaaaagt ggaggagctt cttctgcttg
     2521 cagcccacga tcatacgcgt gtactgcgta atccggagcc ctttgtcgtc ttccgggagt
     2581 tcggcccgaa agcgctcgaa ttcgcacttt atttctggat cgatatgacg aacgcttcga
     2641 cgctcaaagt gtcgagcgat ctgcgctatc gcctcatcgc cctctttgaa aaaaacggga
     2701 tcaagatcgc ctttccgcag atggatgtgc atctggatac tctctcgcct gtcgatattt
     2761 gtctgaaccg tccgcgtact gccgatactg aacaaacaca tacacaggag gaatagcatt
     2821 tcatgctctc gcttgcagaa aacgacctgt actcgctgct tcttgatctt gtcgccatac
     2881 ccagcgtctc cttctccgag gaggagaacc gggctgcgga ttttattttt tctacgctct
     2941 cggatcttga gtactttcgg cgaaacccct cctttctgcg tttcctccca gcagagggag
     3001 acccgctcgg acgcaaggcc gtcgctgcgc tcgtgaaggc gaacccccgg acgaaaaaga
     3061 ccgtcattat tacgggacat ttcgacgtgg tggatgcgga agcgtgcggt cacctcaaaa
     3121 atttggcatt ctcccccgaa gagtataccc gccgtgtgga cgaactggag ataccggagg
     3181 aggctcggga cgatctggcg agcggagagt atctcttcgg gcgcggagtt tccgacatga
     3241 aatcaggaat agcgcttgag atgtgcctta taggagcgct ctcacgtttg gagagtttcc
     3301 ctgcaaacgt tatgtttctc gctgtccccg atgaggagaa cacctcggca ggaatgcggg
     3361 gggctgtccc ctggcttgta cgtcttcgta atgaatggga gctcgaatac acagcgtgcc
     3421 tcaacagcga accgtcggtc ggcgggcggg gcattcctgc cggaggcgtc tacgtcggta
     3481 caataggaaa gataatgcca ttctacctct gcgtggggaa ggaggcgcac gtcggcgact
     3541 actatgaggg ggtcagcgcg tcgcttttaa cgtcgtatct ttcgctcatc cttgagggaa
     3601 gcccggagac tgcggaatcc ctggacgggg ttcattttcc gccgcaggcc tgccttcgct
     3661 tcaaggatct ggtaaaaaac tattctgtga cgctccccga acgctccgtg gcgtattaca
     3721 actgcctcac cgtttcccgg acgcccgccc ggattcttga ggaaatgaag acggccgccg
     3781 gacgggctct tgagatgacg cttacccacc tgtcgaaagc caggcagacg ctgcgcgaac
     3841 gaggggcttc gctccccgag aagaaggcgt tccctccgcg cgtcctttcg tacggagatc
     3901 ttctggagaa agtccgtagc cggacggagc gattcgacga cgtgcttgaa tcttttctga
     3961 agacgcttcc tgactccatg gacgagcgcg atctctgtat cgaaacggcg tcctttcttc
     4021 tcgatctttc cggggagaag gggccgctca ttctcgtcgg atttttaccg ccgttctacc
     4081 ctccgcgcct caacaggagc gagagcgccg gagaacgcgc tctgctgcgg gcggtggaac
     4141 gtctcaagga ggctggaaag ccgtatggcc tttccataag tagaatcgat gtttttcaag
     4201 gaattatgga cttgagctac ctcgggtttc aaggagcgcc gtcggagctt gacgccctgg
     4261 cggagaatat gcctctctgg gggagagggt accgtttccc tcttaacgaa ctgaaaaaac
     4321 tcgacgttcc gatagcaaac ttcggcccga tcggaaagga tgaccacaag aacgccgagc
     4381 gtattcacct gccgtactac cttcacacgc ttcccccgct ttttttcaaa ttcgtggagt
     4441 ttctcgctga agaatcttaa aaagagggtg gagctactga tcaatactga ttataaaagc
     4501 gggttttcgg agacgaaaac ccgcttttgc atttcaaagt atatacagga aaccttctcc
     4561 tcttctgagg gtttatattt ttgtttttcc ggagttgaga aaaagaggat tatggtggat
     4621 aatgattcaa tgaaagaatc aattctggaa tagcaatagt ggatgcacta ctttttgtaa
     4681 acatcgtttc gcacgcctta gaaatagtga aacgaaattt actatacctt cagaaagagg
     4741 tggcatgatg tctcgaaaca gatcggaaga gcttatggag atggaacacg aatatggcgc
     4801 ccacaactat catccgcttg aagtcgtgat ctcgaaagcc gaaggtattt gggtcgagga
     4861 cccggagggg cggcggtaca tggatatgtt atccgcatac tccgcagtca accaggggca
     4921 cagacatcca aagatcatca aagctttgaa agatcaggct gatatcctca ccttgacctc
     4981 ccgcgccttc ttcaacgacc gttggccggt cttcgcgaag aaacttgccg atttcacggg
     5041 aaaagagatg atactcccca tgaataccgg agccgaggcg gtggaaacgg ctttgaagac
     5101 tatgagaaaa tgggggtaca tggtcaaggg agttgagaag gacaaggcgg agatcatcgt
     5161 ctttgaaaac aactttcacg gacgaaccat taccattatt tccttctcga tagatcccga
     5221 cgccacggat tattacggcc cgttcacccc cggattcaaa gtagttcctt acgacgacct
     5281 cgaagcggtc gaaaaagcgg tgacgaaaaa tacagtcggc attatggtcg agcccatcca
     5341 gggtgaggcc ggcgtcgtcg ttccgagcga cggtttcctg aaggggctgg agacgatcgc
     5401 caggaagcac aatgtgcttc tggccatcga cgagattcag accggtttct gccgcacagg
     5461 gaagaatttt gcgttccaac acgagggaat cgatcccgac atcatcatca tgggaaaggc
     5521 gcttggcggc ggagtctttc cggtttccgc catagcggcg aacaaggatg tactgggtgt
     5581 gttcaagccg ggaacccacg gttcgacctt cgggggcaat ccgttggctt gtgccgttgc
     5641 cagcgctgct atcgacgtgc tcgtagacga aaagatggta gagcgcgcgg ccgagatggg
     5701 aaaatacttc atggacggta tcaaggcgat tcaatcgccg aagatcaagc ttgtgcgagg
     5761 taaaggcctg ctcatcggcg ttgtcctcaa cgagtcggca gggaaggcca ggctctatac
     5821 gacggcgctg aaagagaagg gactcctctg taaggagacg cacgactgga tcatccgttt
     5881 tgctccgcct cttgtcatca caaagcaaga gatcgaccag gcgctgaaga cgatacagga
     5941 agtgtttgct tcgacgccga tcccctgcgt gtaaaagaat ccgccccgat gcgtttgcgg
     6001 cagcttcctg aagaggctgc cgcaaacttt atgggatgca actcgcttgc agtgaatcgc
     6061 gtgttcctta aatgtatgaa aaaatgacgg acgtcaacga agctccaaaa cagaaattgt
     6121 ctctacggaa aacttctttc ttgaagagcc ccaaaagagc cgaaaatatt tgagagatag
     6181 agcgcgacgt agtaaaatga cttctgaaaa gcagtgaaac cgtacggtgg aacacaaaat
     6241 tctcaggagg tggttggttt tgaaacggaa ttggatgatc gctctcgtag tgcttgttgt
     6301 cgtcgccggg tatttcttta tgcaacagca ggcgccgcag cccccgaagg aggagccggt
     6361 gaaggaatct cctacgccga cgcccgaggc gccaaaggct gaagagccaa aggctgagga
     6421 gccgaaagca gaagaaccga aagcgtctga agagactgtg aagatcggct atcttgccgc
     6481 tcttacaggc gactgggcgg cctacggtca gaccgaggag aaaacggccc gtcttgcagt
     6541 tgatgaaatc aacgcaaaag gcggcgtcct tggaaagaag ttggagctcg ttgtctatga
     6601 cttccgtacg cgcgccgaag atgctgtgaa tgccgtccgg cgtatgatcg aagaagacaa
     6661 ggttgtggct atcgtcggag ccaacggcag cggcatcaac atcgctaccg cgcccattgt
     6721 gaaccgtctc ggcgtatccc agatcggcac cgtttcaacg aatcctctcg tcaccgtcga
     6781 tgagaagggc gaggttcgtc cgtactcatt ccgtatatgc ttcaccgacc cctaccaggg
     6841 aaagctgatc gctttctttg cggcgaaaga acttaagaga atgaaggccg ctattctgta
     6901 tgacgtgggg aacgactatt ctcagggcct tcgtgagttc ttcatgaaga gctacgccga
     6961 gtaccagggc gaggttgtcg ccgacctcgg attccgcggc ggcacggatg tcgacttccg
     7021 cgcgcagctc accgagatcc gcgacaaggg cgccgatgtg ctcgtccttc ccaacatggg
     7081 caaggagatg gcgctcatca tgaagcaggc acgcgagctt ggtatgaacg aaatcgtctt
     7141 cgtcggcggc gacgggtacg gcgagttcat gtgggagatc gcaggagagg ccatggaggg
     7201 ctcctactgg attaaccatg tcgctcccga ggatcctgcg atggcgccct tcttcgcggc
     7261 gtacaaggaa aagtgggatg acgagtgcaa agagttcgtg aacggtgtgc tcggctatga
     7321 ctctatttac tgggtggccg acgcgatctc ccgcgccggt aaggccgatc cgaagctcat
     7381 ccgcgacgcg cttgaggcca ccaaagatct gaagcttcac catgccacca tcaccatgga
     7441 cccgaagacg cacaaccctg tcgacaagga cggcgtcgtt ctcatagcca aggacggcaa
     7501 gggacagttc ttcacgaaga tcaagccgta agtgcaccgc agagtttaaa taatacaata
     7561 taccgtgaag aaaaaaatat gatatagttg ttccatgacg agggtaggcg gagaccgtct
     7621 gccctcgtca atacgtgaaa ggggtgatcc atatttggca acgctgatac agcagatcat
     7681 caacggcctc tcgcttggat cggtctacgc actcattgcc gtgggatact ctctggtcta
     7741 ttccgttctc ctcttctcca acttcgccca cggaggcttt cttgtaatcg gcggttatat
     7801 ctgctacgct gcgcttcgta cttttaacat cggaatatgg atggcgggtg cgctttctct
     7861 ggtaggagcg ggagcggcgg ccattctcac ggaacggctc gcctatcgcc cgatccgcga
     7921 acgcaccaat gtgactcttt atcttcttat tgcctcgatg ggaatgagca ttgtcatcga
     7981 aaatatcttt gtcattgtgg cgggaggacg tttccgcgct cttcctccgg tgatcccgac
     8041 cacgccggtg aactttttcg gtcttgccac tacaagcgcg ttcgatcttc tttcccttgt
     8101 ttcggctgtt gttttcctcg gcgcattgca gctcttcctg tcgaagaccc gatgggggct
     8161 tgccataagg gctgctgcgt atgatcttcg aactgccggc ctcatgggag tgaacgtcaa
     8221 tcgtttgatc tccatcgtct tcttcgtcgc gggagcgctc gctgccgtgg gaggaatttt
     8281 cctctccgtc cgctacacac tgtaccccca gctgggcgca atcacaatca aggcatttgt
     8341 agcggctgtt atcggcggtc ttggttccct tcccggcgcc gtggtgggca gcctcatcct
     8401 cgggcttgcg gagatgctca cggccggttt catatccagc cagatgcgcg acctcgtcgt
     8461 cttttccctg ctggtcgtca cgttgcttgt caagcctacc gggcttttcg gcaagagcgt
     8521 cagcgagaaa gtgtaggtga gcgaagacca tgtcaggtta tgcggaaggt attatcattc
     8581 ttttgtgcat caacgcaatc gcagccatgg gagtctctct cctcacggga ttcacgggca
     8641 tattctcttt ggggcatgct gcgtatatgg cgctcggagc ctatacgtcg gcgattctta
     8701 cagtacagca cggcgtccat ttcttccccg cgattcttct cggcggagtg gtggctgtcg
     8761 cccttgccta ctttatcggc gttccgacgc tcagactcat gggcgactac tacgctatag
     8821 cctccatcgg ccttggtgag gccatcagac ttatccttga aaactggcag tctgtgacgc
     8881 ggggagcccg agggtatccg gggatcgatc cgtacacctc gctgcctatc gcagtcgggt
     8941 ttttcgtcat actggccatt ttcatgttca accttatcaa cagccgtttc gggagagcat
     9001 gcaaagcctg ccgggacgac tacatcgcct cgtcgctgct tgggttcaac accgcgcgaa
     9061 tcagagttgt cagtcttatg atttcggctt tctactgcgg tgtcgccgga gcgcttctcg
     9121 gtggttttct ttctttcatt cagccggtca tgttcgacat gctcaagtcg acagaattga
     9181 cagcggtcgt cgtcttcgga ggcctcgggt caatgagcgg ctgcatcctg ggaacggcgg
     9241 tcatcacgtt ggttaccgag cttttccgtc ctatctccca gtaccggatg cttatttacg
     9301 gggctgtcct cgtgatcatc atggttcttc gtccggaggg tatcatgggc aaccgtgagc
     9361 ttctctcctg gattaaggat cctttcggga ggcggtcgtg atgagcgcga tcttcgaact
     9421 ttccggcgtt acaaagaact tcggcggcgt gcgcgctgtg agcgaaatgt ccttttcaat
     9481 tccggagggc gagctggcag gccttatggg accgaacgga gcgggcaaga ctacgatctt
     9541 caatcttgta accggcgtct acgatcccga cgaggggtcg attctcttcc gcggtgagaa
     9601 cctggttcct ctgaagacgt tcgacattgt ccacaaaggg attgccagaa cctttcagaa
     9661 cctgcggctg tttcagcgtt cctcggttct ggagaatgtc atgaccgctg cgcagcagca
     9721 tgtcccctac acctttttcg agggaatgac gcacctcgga cggtggcgga gcaaagagag
     9781 cgacatacgg aagaagagca tggaactgct cgaaagggtt ggactcgccg acagagcgaa
     9841 ccaggctgcg gggacgctcc cttacggaca ccagcggcgt ctggagattg cccgtgcgct
     9901 cgcgctggac ccgaaattgc tgctgcttga cgagcctgcg gccggaatga acgccgaaga
     9961 ggttctctcg ctgaactctt tgatccggtc cattcacacg gatttcaaac tgacgatcct
    10021 cgtcatagaa catcacatgg atctcatcat ggagatctgc ccccatatcg tgtgtatgaa
    10081 cttcggcgcg aagatcgccg aaggaacgcc ggatgagatc cagaaccatc cggaagttct
    10141 caaggcgtat ctcggagagg aggaagagga atgagcgacg cggttatgaa cgagaaaaaa
    10201 gccctcctcg aagtccatga cctccacgtg agctacggcg ccatcaaggc tctctccgga
    10261 gtctctctga aggtgttcga aggcgagatc gtcagcgtca tcggttcgaa cggcgcgggc
    10321 aaatctacgc tgatgaatgc tatcatgggt atggtgcggc gagaaaaagg agaaatcctt
    10381 cttggcgacg taaagcttga ctcgagaagt ttcagggtgg tacagcaggg gatttcgctc
    10441 agccccgagg gccgcaaggt cttcgcccct ctcactgttc tggaaaactt gaagatggga
    10501 gctttccctc ggggaaatga cagtaaagtc gaagaagact atgaatgggt cttctccctt
    10561 tttccccgtc tgaaggagcg gattagccaa tacgcgggca cgctttcggg aggagaacag
    10621 cagatgctcg ccatcggcag ggcgctcatg tcccgtccga aggtgctcct ccttgacgag
    10681 ccttcgctcg gacttgcccc cattatcatc aaggatatct tcaaagagct gcgcaggatt
    10741 aataacgaag gagtcacaat cctgctggtt gaacagaacg cccgccaggc tcttctgctc
    10801 tcaaatcgcg gctatgtgat tcagaccggg cgtctggtca cggaaggtcc ctcggaggaa
    10861 cttcttgcga atgcggacat caaggcggcc tatctcggcg ggggccacgc gaaggggaag
    10921 acgacccaag cgcttgcctg atccttgctc tctcgctgaa tttacgagaa acacccggtc
    10981 tctccggaag accgggtgtt tttttcgata ataaacttga caaaaatagt aggatatagt
    11041 actctgtcgt cagttcagag caggggaagt gatggcaatt aaactctcga caaaaactcg
    11101 ctatggtctt cgcgccttgc tctatatggc tgaaagatac gacggtgaac gaactgtgcc
    11161 cgtcggcgag atcgccaagg agcaggaagt ttccgaaaaa taccttgagc agctttttct
    11221 caagctcaga cgcagcaacc ttatacagag cgtccggggg gcgcagggcg gctatatgct
    11281 cggcaggccg cccgaggata ttacgatcgc atgtgtggtg gattctcttg aaggggatat
    11341 tacctttgcg gactgtcttt cgccgggcgg atgcagaaat cagggttcct gcccgacgca
    11401 cgaactctgg agccgtttga aaggaagtat cgacgagatc ctggaggata ccacgctcgc
    11461 ggatcttcta aaaaattcag cgacgaacca cgccggggaa aaatccggca agggaagttg
    11521 ttcaagcaaa tagaaggggg aaaaaaataa tgtccagaca ggtctatctt gattacgccg
    11581 ctactacgtt tactgcgccg gaagtcgttt ccgcgatgac cccgtacttt acgcagtcgt
    11641 tcggcaatcc ttcgtcgatc tattcttttt ccgagagcaa ccgcaaggct atgggcatag
    11701 caaggcagca ggtggcggat gtgctcggcg cggcgaagga agaaattttt ttcacgagcg
    11761 gcggcactga agccgataac tgggctctca aaggggtagc gtggcgaaat cgttcaaagg
    11821 ggaaccatgt tattacgacg aagatcgaac atcatgccgt gctgcatacg gcacagttcc
    11881 ttcaggagaa cggttttgaa gtgacgtacc tcgacgtgga cgccgaaggc agggtgcgtc
    11941 ttgaggacct caaaaatgcg gtgaccgaga agacaatact tgtttcggtg atgtttgcca
    12001 acaacgaaat cggcacaatc caacctatag ccgagatagg cgcattctgc cgtgagaagg
    12061 gaatcatctt ccacacggac gcggtccagg cggtggcgca tgtccccatc gacgtgaagg
    12121 cgatgaatat cgacttgctc tccctttctg cgcataaatt ctacggtccc aagggagtcg
    12181 gggcgctcta tgtccgcaag ggaataaaga ttgacaactt catgcacgga ggcggtcagg
    12241 agcgcggaaa gcgtgcgacg accgagaacg tggcgggtat cgtaggactc ggcgcggcaa
    12301 tcgaactgac tgcgtccagg atgaaggacg cgtcttcccg cgtcggaaag ctccgtaaca
    12361 tgctgatcga cggcgtgatg aaggttatcc ctcatgcgaa gctcaacggc gctccagtaa
    12421 gcgagcggct tccgaacaac tcgaatttca gctttatcgg gatcgaaggc gaaacgcttc
    12481 ttctcgacct ggacggggcc ggtatctacg cttccaccgg cagcgcctgt tcctctggat
    12541 cgctggatcc gtcgcacgtt ctcatggcga tcggcctttc ccatgaacag gcgcacggtt
    12601 cgctccgtat gacgctcggg gaggagactt cggaagagga tatccgctat gttctctccg
    12661 tgcttccggc aatagtagag cgccgccgcg ctatgtcgcc tctctgggaa gactttctga
    12721 agaacacgca aggagggaaa taacgctatg tacagcgaaa aagtattgga tcactttcaa
    12781 aatccgcgca acgtcggtga aatcgagaat ccggacgggg taggccaggc aggaaacccc
    12841 aagtgcggcg atatcatgaa aatatacctc cgcgtggaga acgaagtaat caccgacatt
    12901 aagttcaaga cgttcggctg cgcctccgcg atcgcatctt cgagcatggc gaccgagctt
    12961 gtgaagggac ggaatatcga cgaagcgtgg aatcttacga acagcgcggt tgcagaggcc
    13021 ctcgacggac tgccccctgt gaagatgcac tgttccgtcc tcgcggaaga agccattcac
    13081 gccgcaatca acgactatcg cagacggaag gggctgcctg tgctcgaggg taaagagtac
    13141 gatcacgagc acgatcacgc tcatcagcac tgagccgtga acggcgcttt caatcgctgc
    13201 ccgtggccgg ggaccgaccc cctctacgtc gcataccacg atgaggagtg gggcgttccc
    13261 gtccgcgagg acgcgacgct ttttgaattt ctcctattgg aaggcgcgca ggccggtctc
    13321 tcctggatca ctgtcctccg gaaacgggaa gcgtacagga gcgcgttcga cggttttgat
    13381 ccggagataa ttgccgggta tgacgaacga aaaataggcg agctgatgcg gaacgaggga
    13441 atcatccgga acaagcggaa gatagagggc gccgtgaaaa acgcacgcgc cttcctccgg
    13501 atcagggatc ggaaaggctc tttttcggca tacttatggg atttcctcga cggggagccg
    13561 atcgtcaaca gatggaaaac catggaagaa gtgcctgctg tgaccccgct cgcggagaaa
    13621 gtaagcgggt gcatgaaaaa agaggggttc gtcttcttcg gtccggtgat ttgttacgcg
    13681 catatgcagg ctacgggaat ggtgaacgat cacctgacga gctgttttcg ccaccccgag
    13741 tcggggtatt gaagcgggaa gggatgacag gatcggggga cgcttccgtc attttcccgc
    13801 ttctttttaa gcctagcgaa gtccttcgaa cgggccgcgc caccgcatct ccgtctgtga
    13861 gaaggagggt tccgcaagat cggccttcct tgaagcggag aatccttcaa agggccctct
    13921 ccaactgacc gagaacatat cgtcgagatg acgatcattt cttctgaaga cctctctctc
    13981 tgtttccgtc gccgctgtga atccgtactt tcctgccatt gtacacactc ccttcaaaat
    14041 tataaaaaga cgcagagtaa aaacacttcc caataaaaaa accgggggcc tccagaaagg
    14101 cggcccccgg ttccggtttt aggtgtacgg cttatgcgcc gaacccaccg gaagggggac
    14161 cgccgataat gaggagtacg aggacgagta caagaacaga aatacgaacc aaactcaatc
    14221 tctgtgcatt catcaaatgg cctcctttcc ggtgaagtgt ctgtctgaat attgataggg
    14281 atgatacagc ccctgcggta tcctgtcaag catctatttc cagacttttt acgtaaattt
    14341 acgttcccgg ggtgcaaaag taggatttgg gatgaaaaat gtgggtacat ccggaacgac
    14401 acgctcattc caagctttcc agagcacgct gttcgggagt ataattccct cttgttgttt
    14461 acttatttct gcgaggtgtt tcctgtggtg aatgggttct tcgtcttttt tgtttttgcc
    14521 gccgtctatc tggggatgat ctttgggcgc tttcctcgcc tcgcgcttga ccgcaccggt
    14581 attgtccttt tgggcgcaat cgtgcttctt gccgcgggaa aggcgtctct cgagggcggc
    14641 cgctccggga tcgacgtccc cacaattttg ctcttgttcg gctttatggt tatttcggcg
    14701 cagttccgtc tgggaggttt ctactccgag gtttcccggc gcataggggc tctcgcggcg
    14761 cctccttcgc ttatcatggg ggcggtcatc ggagcttccg ctctcatgtc ggcgctcctc
    14821 acgaacgatg tggtctgtct cgccatgacg cccgttctgg gggcggggtg catccgccgg
    14881 gggctcaacc ctcttccttt tttccttgcg cttgcgtgcg ccgcgaatat cggttccgcg
    14941 ctcacgctca tcggaaatcc gcagaacatg ctcatcggcc agtatctctc gcttcctttc
    15001 ggcggatatc tgctcttcgc cgccttcccc gtgttcctgt cgctgctttc actctggggg
    15061 atcatgctct tccttttccg gggacgctgg acgctccaaa tggatgagca gctggttgat
    15121 gagcgctcct ttgatgcctg gcagacgttg aaggggggtg tcgtcctgac ggtagtcgtg
    15181 ggcttgtttc tcttctcgac gcttcctcgt gatctcacgg cgctcggcgc tgcgggggtc
    15241 cttctgctca gcaggaagat ggcgtcgcgg cggaccctcg aaatagtgga ctggcagctt
    15301 cttctccttt tcctcggcct tttcgtcgtc aatgcggcgt tcgagtctgc cggaggtctt
    15361 cagattctca aagagttcct tgaatcagca ggggtatcgc tttcctctcc ggcctggctt
    15421 tacggagtga cgacagtgct ctcgaatctt gtctccaatg ttccggccgt catgctgctg
    15481 ctcccacttg tgcgcgacgt tccgcaggcc ggttacatcc ttgcggtgtc gagcaccttc
    15541 gcgggaaatc tgctgatcgt ggggagcatc gccaacatca tcgtcgcagg cgaagcggag
    15601 cgtatgggga ttccattcga ctggaagcag cacgcgctcg tgggagttcc ggtgaccctg
    15661 atcagccttg ccgccggact cgcctgcctc tggctttttt aaaaaatcga tatcatgaat
    15721 actcgttgaa ttgtccatag aatcaacttt attcccttgt cttttgatag gaaagtggta
    15781 tattgacggg gtgacttccc ggaaggcccc cattctggaa acggaggagc aagatggatc
    15841 tctcgatatt gttcagaggc gttgttatcg ggttttcgat cgccgcgcct gtcggtccca
    15901 taggagtttt atgtatccgg cgcacgctga ccgaagggcg tgcgtcaggt cttgtttcag
    15961 gactgggcgc agcgagtgcg gatgcggtgt acgggtgtat cgcgggcttc ggcttgacgt
    16021 ttctctcgaa ctttctggtc agcaggcaga cggagcttcg ccttgccgga ggtcttttcc
    16081 tgctgtatct ggcggtcaag acgtttttcg cacagcccgc acaaaaagcg gccgaggtac
    16141 gcggacacgg cctacttggg gcgtatctct ccaccttgtt cctgacgctc actaatccga
    16201 tgacgattat ttcgttcgcc gccatatttg ccggactcgg actggccgaa gggagcggaa
    16261 actatatatc cgccggagta ctcgtcctcg gggtgtttgc gggttcagct ctctggtggc
    16321 ttcttttgag cggcggcttc gg
//
LOCUS       DAOPKU010000004        16237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_3783_length_16237_cov_4.922630, whole genome shotgun sequence.
ACCESSION   DAOPKU010000004 DAOPKU010000000
VERSION     DAOPKU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 16237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16237
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3783_length_16237_cov_4.922630"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK849_00240"
     CDS             complement(<1..637)
                     /locus_tag="PK849_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPE64577.1"
                     /translation="MNDSYASSDGFPALIRILSEASEWIVLSHVKPDGDALGSASAFA
                     SVGRKLGKTVVWGGPDEYPSRYLFLEGSGEFRPNLKLSSLALTEGSVVLALDTSTRAR
                     SVEDLDALPENVPLLNVDHHADNELFGTVNYVDPSSSSTGEIAWLLLREWGIPLEIDV
                     LEAVYTALATDCGNFRFRVLRRGPTGLQRNFLTEGFLRQSWMSESGAMHPFP"
     gene            complement(654..1067)
                     /gene="rbfA"
                     /locus_tag="PK849_00245"
     CDS             complement(654..1067)
                     /gene="rbfA"
                     /locus_tag="PK849_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPE64578.1"
                     /translation="MTTFRMQRINKQIQREVSLLLELRIKNETAKKAIITDVDCSRDL
                     EHAKVYFTTVDRSEREEVGKALGTVAGALRSQLGKLLTIRQIPALSFFFDTSEEYGRS
                     IDVLLDSLKTEDSVIERDEDDETEDASDTDELPEA"
     gene            complement(1380..3365)
                     /gene="infB"
                     /locus_tag="PK849_00250"
     CDS             complement(1380..3365)
                     /gene="infB"
                     /locus_tag="PK849_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPE64579.1"
                     /translation="MSKIRVYELAKMLGKSNKELLDLLIDLGADVKSHMSSIDNEIAQ
                     LVEDALSSTSSSGTQKTKKEEQKVTKTETFEVSKGSTVKAVAELLGVTPAEAVKSLIS
                     AGMMVPADSPVDERCLEVLGEAYDAVLTLACDDAEQEGEAATCAVIKRKTFSGDHLEA
                     RSPIVTVMGHVDHGKTTLLDFIRKTNITAREAGGITQHIGASIVMHEGRRIVFLDTPG
                     HEAFTSMRARGAQATDVAVLVVAADDGVMPQTREAINHAKAAGVPIVVAINKVDKPAA
                     KADRVRQQLSDVGLAPEEWGGSTIMVEVSAKSGQGIPQLLEMILLVSEMEDLKADPTV
                     RPEGVVVEAKLDKGKGPVATVLVQQGSLCKGDIVLFDTAWGKIRAMIDAEGNPIDCAG
                     PSTPVEILGLTAVPQPGEVFKAVENERIARDALSVKEQEVRNAQQSGPRKLTLEELYS
                     QLKEGDIPQLNLLLKCDVQGSSEALAASLEKLATNEVGINIVHRGVGRIAESDVMLAS
                     ASNAVIIGFNVRPDANAKKVAESEGVQVRLYDIIYDAIDDVKAALEGMLKPTIRENTL
                     GQAEIRQVIRVPKAGNVAGSYVLEGVIRRNAKARLVRNGIVLWDGSLANLRRFKDDAR
                     EVAAGYECGISLSNFQDFEEGDIVEAYEIIEEKRHLS"
     gene            complement(3358..3696)
                     /locus_tag="PK849_00255"
     CDS             complement(3358..3696)
                     /locus_tag="PK849_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64580.1"
                     /translation="MNEQALATLFSLLGFARRSGSLLIGQDIVKSSLVRRESLLVLFA
                     EKDSSFYRSLQKGRYAEKCLLLNPPGISEADLSAAIGSRNVKVVALPLRGGFAQRILQ
                     LLAEGGERIE"
     gene            complement(3693..3977)
                     /locus_tag="PK849_00260"
     CDS             complement(3693..3977)
                     /locus_tag="PK849_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HPE64581.1"
                     /translation="MKRRRPRTCVACREESPKREMLRVVRTPEGSVRLDLSGKAPGRG
                     AYVCVSEECLAAARKRDALAKALKTKVEDAVYEEILAVLREKKKERAETP"
     gene            complement(3989..5092)
                     /gene="nusA"
                     /locus_tag="PK849_00265"
     CDS             complement(3989..5092)
                     /gene="nusA"
                     /locus_tag="PK849_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPE64582.1"
                     /translation="MQLGRDFARALAQITKERGLSQEVIASSLEAALISAYKKFQGGN
                     QDVEVFIDTENGDISICEVKQIVDVVTSPDVQISLEDAQSQGFVDVAEGDYIRIERDP
                     ENFGRIAAQTARQVIIQRLKDAERQIIFEEFSDRVGDLVTGVVFKSENDQVLIRLNDR
                     TEAILPREERIVSEKYIPGERMKLYLLDVRQTTRGPRIVVSRTHPGLLRKLLELEVPE
                     IREGVIDIKNIVREAGARAKIAVQTLDVNVDPVGACVGNNGARIKSISKELCGEKVDV
                     IVWSSDPLQYVRNALSPAKIVKIEPILEQEKAVTVYARPDQLSLAIGKAGQNVRLAAR
                     LTGWKIDINALEPERMPTLHDIFQDIIEEEGSK"
     gene            complement(5094..5594)
                     /gene="rimP"
                     /locus_tag="PK849_00270"
     CDS             complement(5094..5594)
                     /gene="rimP"
                     /locus_tag="PK849_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPE64583.1"
                     /translation="METGRKDGWKSLRAIVEGLGYECVGIALVTEERAVFLRVFIDSV
                     GGIQVRDCEIVSKAVSAFLDEHEEYIPGKYFLEVSSPGIERPLFTPEDYKRFIGKKAR
                     LRVRLPVQGRKSLSGLILSVDDDAVELEIDADTPEEMTAVRISFDIITKGNLAFEEKK
                     DKRRNS"
     gene            complement(5727..5915)
                     /locus_tag="PK849_00275"
     CDS             complement(5727..5915)
                     /locus_tag="PK849_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64584.1"
                     /translation="MAGAKVFERNPYEDVVYWPGVIALPELMEDAMADDLLFADDPME
                     FETYLGEWWEGFVSDFYL"
     gene            complement(5954..7429)
                     /gene="guaB"
                     /locus_tag="PK849_00280"
     CDS             complement(5954..7429)
                     /gene="guaB"
                     /locus_tag="PK849_00280"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPE64585.1"
                     /translation="MSLESKFVPYEGFTFDDVLLEPAFSEVLPSDVSIKTNLTESIAL
                     NIPLCSSAMDTVTEARLAIAMAREGGIGIIHRNMPLEKQAKEVDVVKRSESGVIVDPF
                     FLHPEDMVREAISLMEHYHISGVPIVDAKKRLVGIITNRDLRFVQNFDQPISRIMTKD
                     NLVTASEGTTLEGAQSILMKYKIEKLPIVDENGILKGLITIKDIQKAKDFPNAAKDSG
                     GRLRVGAAVGVGADLFERAAALVKSGVDVLVVDTAHGHAKKVLDSVKNLRGAYPEMTI
                     IGGNIATAGAAEALIDAGADAVKVGVGPGSICTTRIIAGIGVPQLGAIYNVALAAHAR
                     GKTVIADGGIRYSGDIVKAIAAGADSVMIGSLFAGTEESPGEPIIYRGRSYKSYRGMG
                     SLGAMKEGCSKDRYFQESAVGDKLVPEGIEGLAAHKGSLSGVVFQLTGGLRSGMGYVG
                     ARDVQELQQKARFIKVTAASVKENHPHDVVVTKEAPNYWVD"
     gene            complement(7542..8708)
                     /gene="ispF"
                     /locus_tag="PK849_00285"
     CDS             complement(7542..8708)
                     /gene="ispF"
                     /locus_tag="PK849_00285"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPE64586.1"
                     /translation="MKEASLVIVAAGKGERAGGAGGTAKQFRPLAGIPLWKWSASAGE
                     RLFNKNVLHECILVVPESAVESVQRETLGWKIPFFVVPGGAERQESVVRGIRAASREY
                     VLVHDGARPFLSGALVERLLESLTDEYGVAPLLPVSDALKKTGEDGTFSPFPREGLWV
                     TQTPQAFHREKLLSVLQTHGGGAKDEAEAWIAAGHPLRSVRGERKNIKITWEEDFSLA
                     ELYVQRTFRTGTGYDVHPLVPGRRFILGGVEFPEFPLGFAGYSDGDVLAHAVCDALLG
                     AAGLGDIGTLYPAGNPAFKNISSLLLLSDTAKRVTDAGWILEWSDSVICAQEPKLAAS
                     LSRMVSKMEEVLPETWRGRVHLKVKSGEHVGPVGHSHCIVCYSNATLWKPLDII"
     gene            complement(8705..9886)
                     /locus_tag="PK849_00290"
     CDS             complement(8705..9886)
                     /locus_tag="PK849_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPE64587.1"
                     /translation="MTEGFGKIVQLIFSGLMILIGGMIGAQVAQLLFPIIPAEWFAAW
                     KEWLPLWMPGKILMTIFSVVLFAFIGLMTSPLFLRVLGSMGSFIESHLKNTSWSEITS
                     ATVGMIIGLLIANLLALPFSDLPFGSYLAVVLNVVLAYLGARILLKRQSDLRNVFGPI
                     RSLKERLSMIKEHPHHDDEGFPAEEQPWEVPKKILDTSVIIDGRILDVARTGFLEGII
                     LIPQFVLHELQAVADSTDPARRTRGRRGLDVVNELQRLSDLQIEIVDASLKHLKADSV
                     DSALVVLAEKIGGQILTTDYNLNKLAQIQGIRVLNVNDLANALKPMLLPGETIIIDII
                     REGKEPHQGVGYLDDGTMFVVEDGENYIGKRVEVVVTSMLQTSAGRMVFGRIRREVRA
                     S"
     gene            complement(9964..10572)
                     /gene="recR"
                     /locus_tag="PK849_00295"
     CDS             complement(9964..10572)
                     /gene="recR"
                     /locus_tag="PK849_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPE64588.1"
                     /translation="MALPDSFERLASLFRRMPGVGAKSARRMAFFVLQQPPSFSDELA
                     RVLVALKERVHACSVCGNITDDDPCSICSDPLRDHRLLCVVETVEDLFSIEHAGVFAG
                     VYHVLGGRVSPLDGETLDERVLSALKEHIENDAIEEVIIATNPRIEGDLTFHALVEYL
                     GANNVALSRIAYGLPVGGSIEFADRTTLQAAMDSRVRVRRSS"
     gene            complement(10592..10897)
                     /locus_tag="PK849_00300"
     CDS             complement(10592..10897)
                     /locus_tag="PK849_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPE64589.1"
                     /translation="MNMEKIMKQAQKMQAQMAKIQEDLAGEQVEGSAGGGMVTVTCNG
                     QGDVVAVKIEPEVAGDDVEMLEDLVLAAVNDAIRKSKELANSRMSQLTGGLGMPGLF"
     gene            complement(10919..11380)
                     /locus_tag="PK849_00305"
     CDS             complement(10919..11380)
                     /locus_tag="PK849_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPE64590.1"
                     /translation="MKIIILSVGKPKDVHVVSLAEHYFKRLRSFVPVVLESVPEAKDP
                     LPSKKVEREGVGLLKNLRERDFLVTLEERGKCFDSREFAEWVQTSVERTPGRVVLAIG
                     GAFGLSEDVRRRSDFSLSLSPMTMPHELCLVFLMEQLYRAFTLIRGVEYHH"
     gene            complement(11526..13172)
                     /locus_tag="PK849_00310"
     CDS             complement(11526..13172)
                     /locus_tag="PK849_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPE64591.1"
                     /translation="MDKTQYNYFRDLTLIAAWILAALCVGGPSSRFIVGMAILAAVFG
                     MVQHMYPNSLWKPVYLLIGFIFALFGPRIAFIGLPAGQYHYLPVEVSVLITALWIAAF
                     PVLLQELDNIPGMAGHLLAVSFSLLLTATVFSGQSLQDAFFMSLSGLLFLGVFWSRHG
                     HMYRRMGEALAAMWGVLVAGTSVLGVSKGITFGTLMVLPLGLFAIPIMEASLNFMNRA
                     FSSKSPNAAVLYSKLIGKGLDHPSAVRFITSLCALGGSAVAISQLGHPLSMMFVVSLA
                     ILFAGITLAPAIRDMKENVEMHQRPSIWRTTIDNVSMNYALAKARGALASRSGARLVA
                     TVNALAVMEAEKNDKYANALDGSFLTLADGAGLVWALKLLGTPVQERVTGIDYMTGLA
                     RMAAAEGWPVYLLGSKPSIVEKAAFELQRRFPDLTVAGFRDGYFDEGDVSVIDEIRES
                     GAKILFVAMGIPKQEIWVTENAERLGDLLAVGVGGAFDVVSGTLKRAPELLQKIGLEW
                     FYRLLQEPWRWKRDLDLFVFVIKVLLTKTGLYRSRARRTV"
     gene            complement(13349..14620)
                     /gene="serS"
                     /locus_tag="PK849_00315"
     CDS             complement(13349..14620)
                     /gene="serS"
                     /locus_tag="PK849_00315"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPE64592.1"
                     /translation="MLDIRWIRSNADEVKAYLANRNNDFDIEPLLALDEEKRKLLSET
                     EELKAKRNEGSRKVGMAKSKGEDAVELMEEMRVIGDRIRDIDSTLAEIEEKMNNMLLS
                     IPNRPHESVPVGKDENDNPVVRRWGEPKSFAFEPKAHWDIGEAAGIMDFEKGAALAQS
                     RFTVLKGVGARLERALLNFMLDLHTEKHGYLEVQPPFMVNSKTMQGTGQLPKFADDLY
                     KCENDDLWLIPTAEVPLTNLFAGEILEEEQLPFYCTAYTPCFRREAGAYGRDVRGMLR
                     QHQFDKVEMVKISTPERSYEELEMLTDNAEEVLRLLEIPHRTVCLCTGDMGFGSSKTY
                     DIEVWLPSQDKYREISSCSNCEDFQARRMNTRYRPKGGGKPQFVHTLNGSGIAVGRTL
                     IAVLENYQREDGSVEIPKALVSYMGGVEEIR"
     gene            complement(14613..15104)
                     /gene="ribE"
                     /locus_tag="PK849_00320"
     CDS             complement(14613..15104)
                     /gene="ribE"
                     /locus_tag="PK849_00320"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPE64593.1"
                     /translation="MRVIEGKLTGAGLRFCIVVSRFNSFFSEKLLEGAKDALLRHDVS
                     HHGIDIVWVPGAWEIPLIAKEAALSGKYDAIIALGAVIRGDTTHYEHVCAEMSKGIAS
                     VSMTQRVPVSFGVLTCESMDQAVARSGSKAGNKGFEAAAAAIEMANILKQMRTEKETD
                     QNA"
     gene            complement(15133..>16237)
                     /locus_tag="PK849_00325"
     CDS             complement(15133..>16237)
                     /locus_tag="PK849_00325"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPE64594.1"
                     /translation="TTEAINFMVRRARGLVCVPLAKEFARRLRLDPMVREGTDKHGTA
                     FLVSVDAKEGTTTGISAEERAATARLLADTSSRPDDFYRPGHMFPLEAKQGGVLKRAG
                     HTEASVDLVRLAGLPPAGVICEIMNEDGTMARLPQLLVFAAEYGLKIISIEDLIAYRS
                     KRERLIEKIATVELPTVHGSFVAHAYKNVLDENAERVHIALVKGEVNGRMGVLVRVHS
                     ECLTGDVFGSLRCDCGPQLSAAMEMVEKEGCGVVLYMRQEGRGIGIAEKLKAYQLQEK
                     GLDTVEANAALGFAPDLRDYGIGAQILLDLGVQNIRLLTNNPRKVVGLQGYGLEIAER
                     IPLVIPPNKFNERYLGTKEQKLGHMLHICDLLR"
BASE COUNT         3688 a         4938 c         4025 g         3586 t
ORIGIN      
        1 ccgggaatgg atgcattgca ccggattcgc tcatccaact ttgccggaga aacccctccg
       61 tcaagaagtt ccgctgcaac cctgtgggtc cgcggcgtag tacacgaaaa cgaaagttcc
      121 cgcagtcagt cgcgagagca gtgtacacag cttccagcac atcgatttcc aagggtatcc
      181 cccactcgcg aaggagcagc caggctatct cgccggtcga agaggaggag ggatccacat
      241 agttgacagt cccgaacaat tcattgtccg catggtgatc cacgttgagc aggggcacgt
      301 tttcgggaag agcgtcaaga tcctccacag aacgcgcacg ggtcgacgta tccaacgcga
      361 gaaccacgct tccctccgtc agcgccagcg aggaaagctt caggttcgga cggaactccc
      421 cggaaccttc aagaaacagg taacgggaag ggtactcgtc cgggccgccc cagacgacag
      481 tctttccgag tttccgccca accgaagcga aagcgctcgc ggaaccgagc gcatcgccgt
      541 cgggtttaac atgtgagaga acaatccatt ccgaggcttc agaaagaatg cgaatgagcg
      601 cggggaagcc gtccgacgaa gcatacgaat cgttcatttc atcctccccg tcactacgcc
      661 tccggcagtt catccgtgtc ggaggcgtct tcggtctcgt cgtcttcatc cctctcaata
      721 acggaatcct cggtcttcaa cgaatccagc agcacatcga tactgcgccc gtactcctcg
      781 ctggtatcga aaaaaaagga aagggcggga atctgccgta tcgtcaaaag ttttccgagc
      841 tgactccgca gagcccctgc gaccgttccg agcgccttcc ctacttcttc ccgctcggaa
      901 cggtcgactg tcgtaaagta cacttttgca tgttcaagat cacgagaaca gtcgacatca
      961 gtgatgatcg ctttctttgc cgtttcgttc tttatgcgca gctccagcag aagcgaaact
     1021 tctctctgga tctgcttgtt gattctctgc attcgaaatg tcgtcataac gtgttacctc
     1081 gcgccagtga tataggatcg agaactcccc ggtctcctcc tcgttggtta aaaaggagta
     1141 caccgcctgg agccgctcct ccgccatagc aacccctgaa gcggctgcgg agacagccac
     1201 aaaggcctcg gaccagcttc cgtcgggccc gagatccatg gctgaaacat tccaacgtcg
     1261 tctgatcctg tcgagcaggg attgcgtcac ctgtctccgg tccttcatgc ttctgcttcc
     1321 cattatttcg agggcaacaa acaggaaacc tgagtacgga acgggaaccc ttcggttcat
     1381 taggagagat gccttttctc ctcgatgatc tcgtatgctt ccacgatgtc gccctcttcg
     1441 aagtcctgga agttggagag gctgatgccg cattcgtacc ctgcggcgac ctcgcgtgca
     1501 tcgtccttga aacggcgaag gtttgcaagg gagccgtccc agagcacaat accattgcgc
     1561 acgaggcggg ctttcgcgtt ccttcgaatt acgccttcaa gcacgtagct tccggcgacg
     1621 ttaccggcct tcgggacgcg aatgacttgg cggatctccg cctggcccag cgtgttctcc
     1681 cgaatggtag gcttcagcat accttcgagg gcggccttca cgtcgtcgat agcgtcatag
     1741 atgatgtcgt acaaacgtac ttggacacct tccgactcgg ctactttctt cgcattcgcg
     1801 tccgggcgca cgttaaaacc aataataacg gcgttcgacg cggaggcgag catcacgtcg
     1861 gattcggcga ttctgccgac tccgcggtga actatgttaa tcccgacttc gttcgtcgca
     1921 agtttttcca aagatgcggc aagcgcctcg ctcgacccct ggacatcgca cttcagcaga
     1981 agattgagct gcggaatatc gccttccttc agctgcgagt agagctcctc caacgtgagc
     2041 tttctcggac cgctctgctg ggcgttgcga acttcctgct cttttacgct gagagcgtcg
     2101 cgggctatgc gttcgttttc aaccgctttg aagacttcac ccggctgcgg tacggcggta
     2161 aggccgagaa tttccaccgg cgtgctcggg cccgcacagt cgatcgggtt cccttcagca
     2221 tcgatcatag ccctgatctt gccccacgcg gtatcgaaca acacgatatc tcccttgcag
     2281 agagagcctt gctgcacaag aaccgttgca acgggtcctt tccccttgtc gagcttcgct
     2341 tccacgacaa cgccttcggg gcgaaccgtc ggatccgcct tcagatcttc catttccgag
     2401 acaagaagaa tcatctccag aagctgcgga ataccctgtc ccgatttcgc ggaaacttca
     2461 accatgatcg tggagccgcc ccattcctcg ggcgccagac caacatcgga aagctgctgc
     2521 cgaactctgt ccgctttagc tgcaggcttg tccactttgt ttattgccac aacgatgggc
     2581 accccggcgg ccttggcatg gtttatcgcc tcgcgcgtct gcggcattac tccgtcgtcc
     2641 gctgctacga cgagaactgc aacgtcggta gcctgggctc cccgtgcgcg catcgaggtg
     2701 aacgcctcgt gtccgggagt atccagaaaa acgattcgtc ttccttcgtg catcacgatg
     2761 gaagcgccga tgtgctgcgt tatgccgccc gcctcccggg cggtgatgtt cgtcttgcgg
     2821 atgaagtcga gaagggtcgt tttcccgtgg tcaacatgcc ccatcaccgt aacgatcgga
     2881 gaacgggctt caaggtgatc gccagaaaaa gtcttgcgtt tgatgaccgc gcaggtcgcc
     2941 gcctctccct cctgttctgc gtcgtcacag gcaagagtga gcacggcgtc gtaggcctcg
     3001 ccgaggactt ccaggcatcg ttcatcaacc ggactgtccg cgggaaccat catccccgcg
     3061 gagataagac ttttgactgc ttcggcagga gtcactccca agagctcggc cacagctttt
     3121 actgtagagc cctttgacac ttcgaaagtt tcggttttcg tcactttttg ctcctctttc
     3181 tttgttttct gggtcccgct ggacgatgtg cttgagagcg catcctccac aagctgggcg
     3241 atttcgttgt caatggaact catgtggctt tttacatcgg cgccaaggtc tatcagaaga
     3301 tcgagcagct ctttattgct cttccccagc atcttcgcaa gttcatatac acggatttta
     3361 ctcaattctt tctccccctt ccgcaagcag ttgcagtatt ctctgcgcaa aaccgccgcg
     3421 taacggtaaa gcaaccactt tcacgtttct cgaacctatt gccgcagaca gatcagcctc
     3481 tgaaattcca ggcggattca acaagagaca tttttctgca tacctccctt tttggaggga
     3541 tctgtaaaac gaagagtctt tttcggcaaa gagcacaaga agagactccc gccgcaccaa
     3601 agaagatttt actatatcct gcccgattag cagagatccc gaacgacgcg caaaaccaag
     3661 aagggaaaac agagtcgcga gcgcctgttc gttcacggcg tctccgcacg ctccttcttt
     3721 ttttcgcgca gaacggcaag gatttcctcg taaaccgcgt cttctacttt cgttttcaac
     3781 gcctttgcaa gagcgtctct ttttctcgca gcggcaagac actcttcaga cacgcagaca
     3841 tacgcgccgc gccccggcgc cttcccggaa agatcaagcc tgacagagcc ttcaggagtc
     3901 ctcaccacgc gcagcatctc tcgtttcgga gactcctcgc ggcaggcgac gcaggttctc
     3961 gggcgccgcc gcttcaagac ggcgtcgctt atttggaccc ctcctcttcg ataatatcct
     4021 ggaaaatatc gtgcagcgtg ggcattctct ccggctcaag cgcattgata tcaattttcc
     4081 agccggtcaa gcgggccgca agacgtacat tctgcccggc cttcccgata gcgagggaaa
     4141 gctgatccgg acgcgcatac actgtaactg ccttttcctg ctcaaggata ggttcgatct
     4201 tgacgatttt cgccggagaa agcgcattcc gtacgtactg cagcgggtcg ctgctccaga
     4261 cgatgacgtc cactttctcg ccgcagagct ccttgctgat cgacttgatc ctcgccccgt
     4321 tatttccgac gcatgcgcca acgggatcca cattgacatc aagcgtctgg acggcgatct
     4381 ttgcccgggc tccggcctcc cggacaatat ttttaatatc gatgactccc tcgcgaatct
     4441 ccggaacctc cagctcaaga agttttctga gaagacccgg atgcgtcctt gaaacgacga
     4501 tacgaggacc tcgcgtcgtc tgcctgacat cgagcagata gagcttcata cgctcccccg
     4561 ggatatattt ttcactcacg attctctcct cacgtggaag aatcgcctcg gtcctatcgt
     4621 tcagacggat gagcacctgg tcgttctcgg atttgaaaac cactccggtc acgagatccc
     4681 ccaccctatc cgaaaactcc tcgaaaatta tctgccgttc cgcatccttg agcctctgga
     4741 taatcacctg acgggctgtc tgggcggcaa ttcggccgaa gttctccgga tcgcgctcta
     4801 tcctgatgta atctccctcg gctacatcga caaacccctg agactgtgca tcctccaggg
     4861 atatctggac atccggactc gtcacgacat ccacaatctg cttcacctcg cagatggaga
     4921 tatcgccgtt ctccgtatcg ataaaaactt cgacgtcctg attccctccc tggaatttct
     4981 tatacgccga aatcaacgcc gcctcaaggc tcgacgcaat aacttcctgc gagagacctc
     5041 gttccttagt gatctgagcc agagcacggg cgaagtcacg accaagctgc attttaagaa
     5101 ttcctccttt tatctttctt ctcctcgaag gcaagattcc ctttagttat aatatcaaat
     5161 gaaatacgga cagctgtcat ctcctcggga gtatccgcat cgatttcgag ctcgaccgca
     5221 tcatcatcga cggagaggat gagtccgctg agactcttcc ttccttgaac gggtaggcgc
     5281 accctcagtc gcgccttctt tccgataaaa cgtttatagt cctccggcgt aaacaaggga
     5341 cgctcaatcc ccggagaact cacttccagg aaatacttgc ccggaatgta ttcttcatgt
     5401 tcatcaagga acgcgctcac ggccttagag acaatttcgc aatcccggac ctgaatgcct
     5461 cccacggaat caataaaaac acgcaggaaa acagcccttt cttcggtgac gagagcaatc
     5521 ccgacacatt cgtatccgag gccttccacg attgcacgca acgatttcca tccatctttt
     5581 ctgcctgttt ccatattatc gccccgattc actcttcgta aaaaacaaag agtgggggaa
     5641 atcccactct ttgcaaaaaa tcccgcaaaa aaatcattat agagtatagc acaaaaaaag
     5701 tctttgcgca gcgtcactcc acaattttac agataaaaat cggaaacaaa cccctcccac
     5761 cactccccaa gatacgtctc gaactccatg gggtcgtcgg caaagagaag gtcatcagcc
     5821 atagcatcct ccattaattc gggaagagca attaccccgg gccagtagac gacatcctcg
     5881 tacggattcc gttcaaaaac ctttgctccc gccatatcgc acccccgttc tcgggaagag
     5941 caggcctacc ccatcaatcc acccaataat tcggcgcttc cttcgtcacc acgacgtcgt
     6001 gagggtgatt ctccttcaca gaggccgccg ttaccttgat aaaacgcgct ttttgctgaa
     6061 gttcctgaac atcccgcgca ccgacatacc ccataccgga gcgcaagcct ccagtcagct
     6121 ggaagacaac gcctgagaga gaacctttgt gcgccgctaa cccctcaata ccctccggaa
     6181 caagtttatc accaaccgcg ctttcctgga agtaccggtc cttgctgcac ccttctttca
     6241 tcgcgccgag cgaccccatg ccgcggtaac tcttatagga gcgccccctg tagatgatgg
     6301 gttctcccgg actctcctcg gtgcccgcaa aaagagaacc gatcatgacg gaatcggcgc
     6361 ctgccgcgat ggctttcaca atatctccgg agtagcggat accgccgtcg gcaatgacag
     6421 tctttccgcg agcgtgcgca gccagagcga cattgtaaat tgctcccagc tgcgggacgc
     6481 cgataccggc gatgattcgc gttgtgcaga tgctcccggg accgaccccg accttgacag
     6541 catccgctcc ggcgtcgatg agggcctcgg cggctccggc ggtggcgatg tttcctccga
     6601 tgatcgtcat ctccgggtac gctccccgga ggttcttcac ggaatcgagc accttttttg
     6661 catgcccgtg cgcagtatcg acaacgagca cgtcgacgcc gctcttcacg agagcggcgg
     6721 cccgttcaaa aagatccgcc ccgactccga cagccgcgcc gactcgaaga cgtcctccgc
     6781 tgtccttcgc agcattcggg aaatccttcg ccttctggat atcctttatc gtgataaggc
     6841 ctttcagaat gccgttctca tccacaatcg gcaacttctc tatcttgtac ttcatcagaa
     6901 tggactgcgc tccctccagc gttgttcctt ccgacgcagt cacaagattg tccttcgtca
     6961 taatccggct gataggctgg tcgaaattct ggacaaaacg cagatccctg ttggtgatga
     7021 tacccacgag cctttttttt gcatccacta ttggaacgcc ggaaatgtga tagtgttcca
     7081 taagagagat tgcttccctc accatgtcct cggggtgaag aaaaaacgga tcgacgatga
     7141 cgccggattc agaacgcttg acaacgtcca cttccttggc ctgtttctcc agcggcatgt
     7201 tcctgtggat aatgcctatc ccgccctctc gggccattgc aatggctaaa cgagcctccg
     7261 tcacagtatc catcgcggaa ctgcacagag ggatattcag agcaatcgat tcggtcagat
     7321 ttgtcttgat agaaacatcg gacggcaaaa cctcactgaa cgccggttcg agaagaacgt
     7381 catcaaatgt gaatccctcg taggggacga acttggactc taaactcatt acggtacctc
     7441 cctgcccgtt agggactcgt aaaagatttt aaaggattgt agggctttcc ggaaaaagaa
     7501 gcaactcccc ttacgaaaaa aacaacaaaa aaacgtccgg ctcaaataat gtcaagaggt
     7561 ttccagagcg tcgcgtttga gtaacagacg atgcagtgag aatgtccgac cggtcctacg
     7621 tgttccccgg atttcacttt gaggtgcact ctgccccgcc acgtttcagg gagaacttct
     7681 tccatcttcg agaccattcg ggagagagaa gcggcgagct tcggctcctg agcgcaaatg
     7741 acactgtcgc tccactcaag gatccatcct gcgtccgtga ctcttttcgc agtatccgaa
     7801 agcagaagga gactcgagat gtttttgaac gcgggattcc ccgccggata gagcgtgccg
     7861 atatcaccca accctgcggc ccccagaagc gcgtcgcaca cggcatgcgc cagaacgtcc
     7921 ccgtccgagt acccagcgaa accgagagga aactccggga attcgacacc gccgagaatg
     7981 aaccttctgc cgggaacgag agggtgcaca tcatacccgg ttccggtacg gaaagttcgc
     8041 tgcacataga gttccgccag agagaagtcc tcctcccatg ttatctttat atttttgcgc
     8101 tctcccctga ccgaacggag aggatggcct gcggcgatcc atgcctccgc ctcgtccttg
     8161 gctccgcctc catgagtttg gagcacggag agcaatttct cccggtgaaa ggcctgcgga
     8221 gtctgcgtaa cccagagccc ctctctcgga aagggcgaaa aagtcccgtc ctctcccgtc
     8281 tttttcaggg catcggatac tggaagcagg ggcgccactc cgtactcgtc cgtaagagac
     8341 tcaaggagcc tttccacgag agcgccggag agaaaaggcc gcgcgccgtc gtggacgagc
     8401 acgtattccc gggaagcggc gcggataccg cgcacgacgg attcctgccg ttcggctcct
     8461 cccggcacaa cgaaaaacgg gattttccac ccgagcgtct ccctctgcac cgactctacg
     8521 gcgctctccg gaacgacgag aatacattcg tggagtacgt ttttgttgaa gaggcgttct
     8581 ccggcggaag cgctccactt ccatagggga ataccggcga gcggacggaa ctgtttcgct
     8641 gttccgcccg ctcctccggc acgctctccc ttgcccgccg caacgatgac aagagaagct
     8701 tctttcacga cgcgcgaact tcccttcgta tccgtccaaa caccatccgg ccggcggatg
     8761 tctggagcat ggaggtgacg acaacctcaa cccgtttgcc gatatagttc tctccatcct
     8821 cgacgacgaa catcgtcccg tcgtcgaggt agccgactcc ctgatgaggt tcctttccct
     8881 ctcgtataat atcaattatg atggtctctc cgggaagcag catcggtttg agcgcatttg
     8941 ccagatcgtt gacgttcaac acacgaatcc cctggatctg cgcgagttta ttgaggttgt
     9001 aatcggttgt aagaatctgt ccgcctatct tctcggctaa aacgaccagc gcgctgtcaa
     9061 cggaatccgc tttcagatgc ttcaatgaag cgtcgacgat ttcaatctgc agatcggaaa
     9121 ggcgctgcaa ctcgttgacg acgtcaagcc ctcttcgccc cctcgttctt cgcgcggggt
     9181 cggtggaatc agccacggcc tgcaattcgt gaagaacaaa ctggggtata agtataatcc
     9241 cctccaagaa gccggtgcgt gcgacatcaa gaatacgccc gtcgatgata acgctcgtat
     9301 ccagaatttt tttgggaacc tcccagggct gttcttccgc gggaaaacct tcatcatcat
     9361 ggtgagggtg ctccttgatc atggaaagac gttctttcag gcttcgaatt ggaccgaata
     9421 cgttccggag atcgctttgc cgcttcagta gtattcgagc cccaagatag gccaaaacga
     9481 cgttcaacac aacagccaaa taactaccga atgggagatc ggagaatgga agagccagaa
     9541 gattcgcgat gagcaacccg atgatcatac cgacggtagc tgaagtaatt tccgaccagc
     9601 tcgtattttt gagatgcgat tcgatgaagg agcccataga accaagaacc cggaggaaaa
     9661 ggggagaggt catcaaacca ataaaagcga ataaaaccac agaaaaaatt gtcatcagta
     9721 tcttgccggg catccaaagg gggagccact ctttccaggc ggcaaaccat tcggcgggaa
     9781 taatggggaa aagtaactga gctacctggg cgccgatcat gccgccaata agtatcataa
     9841 gccctgagaa aatcaactgg acgattttcc cgaaaccttc cgtcatactt tcacctcgct
     9901 tttttgacaa atatgtcatc agcttaaact ttttcacagc acgtctcctt ttccaagaca
     9961 caattaggaa gatcgcctga ctcgtacgcg ggaatccatc gccgcctgca gcgtcgtccg
    10021 atcagcgaac tcgatactgc ctccgacagg aagcccgtac gcaatccttg aaagagcgac
    10081 gttgttggcg ccgaggtatt caactagagc atggaatgtc agatcgcctt caatacgcgg
    10141 attagtggca atgatcacct cctcgatagc atcattttca atgtgctctt tcaaggcgga
    10201 caggacgcgc tcgtcgagcg tctcgccgtc caggggagag actcgtcctc caagaacatg
    10261 gtataccccg gcaaaaacac ccgcgtgttc gatgctgaaa aggtcctcga cggtttccac
    10321 cacacagagc aaacggtggt cccgaagagg gtcggagcag atcgaacagg gatcatcgtc
    10381 cgttatgttg ccgcaaacgg aacaggcatg aacgcgctcc ttcagcgcga caaggacgcg
    10441 ggcgagctcg tcggaaaaag aaggcggctg ctgcaggacg aaaaaagcca tacgccgggc
    10501 gctttttgct ccgacacccg gcattctccg aaacaatgat gcaagtcttt caaaggaatc
    10561 aggaagagcc acggagaagc ccgggaacgc cctagaaaag accgggcata ccaaggccgc
    10621 ccgtcagctg actcatcctg ctgttcgcaa gttccttgct cttacggatc gcgtcgttca
    10681 cagcggcaag tacaagatcc tcaagcatct caacatcgtc ccctgcaacc tcgggctcga
    10741 tcttgactgc aacgacatcc ccctgtccgt tgcaggtcac tgtgaccata ccgccccccg
    10801 cgctcccctc aacctgctcg cctgcgaggt cctcctgaat tttcgccatc tgggcctgca
    10861 tcttctgcgc ctgtttcata attttttcca tattcatatc aaaagcacaa ctcctttttt
    10921 aatgatgata ctccacgccg cgaatcagag taaacgcacg ataaagctgc tccatgagaa
    10981 aaacgaggca cagctcgtgc ggcatcgtca tcggcgaaag agaaagtgaa aagtcgctcc
    11041 gacgccgaac atcctccgaa aggccgaacg cgcctcctat cgccagaaca actcttcccg
    11101 gcgttcgctc aacactagtt tgcacccatt cggcaaactc acggctgtca aagcattttc
    11161 cccgctcttc aagcgttacc aggaaatctc tttcgcgaag attctttaaa aggccgactc
    11221 cctcgcgctc cactttcttc gaaggaagcg ggtcctttgc ttcgggcacg ctctcaagca
    11281 caacaggaac aaaagaacga agacgtttaa agtagtgttc cgcgagcgaa acaacatgaa
    11341 catctttcgg ttttccgaca ctaaggataa tgattttcat agtatatgtc cagcacgttt
    11401 ctcgtatttt ctctttgtcg atcgatcctg tgaaccggac aacaaaaaac gcgcccctcc
    11461 gtcttcgcag aaaacatcag ggcgcacata atacagaggg agccaatttt tcagaaggag
    11521 tttcattaca ccgttcgtct tgctcgtgaa cggtaaagac ctgttttcgt cagaaggact
    11581 tttatcacaa acacaaaaag gtcaagatcc cttttccatc tccaaggctc ctgaagaaga
    11641 cggtagaacc attcaagccc gattttctga agaagctccg gagctcgttt cagagtgccc
    11701 gacacgacgt cgaaagcacc gccgacgcct acggcaagaa gatcgcccag ccgttcggcg
    11761 ttttcggtta cccatatttc ctgcttggga atacccatag caacaaaaag gatttttgcg
    11821 ccgctctctc gtatctcatc aatcacggaa acgtcgccct cgtcaaaata tccgtcgcgg
    11881 aaacccgcaa cagtgagatc gggaaaccgg cgctgcaact cgaacgccgc tttctccacg
    11941 atcgagggct tcgatccgag aagatagacc ggccaccctt cggcagcagc catacgagcg
    12001 agtccggtca tgtagtctat tccggtcact ctctcctgca ctggcgtacc gagcagcttg
    12061 agcgcccata cgagaccggc gccgtcggca agggtcaaga acgaaccgtc taaagcgttc
    12121 gcatacttgt cgttcttctc ggcctccatc acggcaagtg cgtttaccgt cgcgacaagg
    12181 cgtgcgccgc tccttgaggc aagggctccg cgggcttttg caagcgcata gttcatagaa
    12241 acgttgtcaa tagtagtccg ccaaattgaa ggtctctggt gcatttccac attctccttc
    12301 atatcgcgta tggccggagc caaagtaata ccggcgaaaa gaatagcgag gctcacaaca
    12361 aacatcatgg agagaggatg tccaagctgg gagatggcaa cggccgaccc tccgagagcg
    12421 cagagcgacg tgatgaagcg gacggcgctg ggatgatcaa gtccctttcc tatgagtttg
    12481 ctgtacagta cggccgcatt cggcgacttc gaggaaaagg cacggttcat gaaattcagc
    12541 gacgcctcca tgatgggaat cgcaaaaaga cctagaggga gcaccatcag agtaccgaag
    12601 gtaatacctt tgctcacgcc gagaacggaa gtgccggcga ccaaaacgcc ccacatcgcg
    12661 gcgagcgcct ctcccattct tcggtacatg tgcccgtgac ggctccagaa aactccgagg
    12721 aagagcaagc ccgacaggga cataaaaaaa gcatcctgca aactctgacc ggaaaagacc
    12781 gtggcggtca gcagaaggga gaacgaaaca gcaagcagat gcccggccat gcccggaatg
    12841 ttatccagtt cctgaagcag cacggggaag gcggcaatcc agagggcggt tatcagtacg
    12901 gagacctcaa ccggcaaata gtgatactgc ccggcgggca aacctataaa ggcgatgcgc
    12961 ggaccgaaaa gagcaaaaat aaaaccgata agcaggtaga ccggtttcca gagcgagttc
    13021 ggatacatat gctgcaccat gccgaatacc gccgcaagga tcgccattcc gacgatgaaa
    13081 cgggaagacg gccccccgac gcacaacgcc gcaaggatcc aggccgcaat aagggtcagg
    13141 tcacggaaat agttgtattg cgtcttgtcc atgctccttc tgcaggcgcg ctgcgcaaaa
    13201 aaacagagca ccgtaagagg aagcacacat aaaagaatgt cggtaacgta catttccgcg
    13261 tggaaaatat ccagttccct cccctgcaaa aaagtctttg atgattgtat cagctgtcgc
    13321 tgtatttttc caccgttctt tgccgaggtt accggatttc ctccacgccg cccatatagg
    13381 aaacaagcgc cttcggaatc tccacggaac cgtcctcgcg ctgatagttc tcgagaacgg
    13441 cgatgagcgt ccgtcccacc gcgatgcccg agccgttgag cgtgtggacg aactgcggct
    13501 tgcccccgcc cttcggacgg tagcgcgtat tcattcttct ggcctggaag tcctcgcagt
    13561 tactgcacga actgatctcc cggtacttat cctgggaggg cagccacact tcgatgtcgt
    13621 atgtcttgct ggagccgaac cccatgtcgc ccgtgcagag acagacagtc cggtgcggaa
    13681 tttcgagcag gcggagcacc tcttccgcat tgtccgtcaa catctccagc tcctcgtagc
    13741 ttctctccgg cgtgctgatc ttgaccatct cgaccttgtc gaactggtgc tgccgaagca
    13801 ttccgcgcac atcccgcccg tacgctccgg cctctctgcg gaaacacggc gtataggccg
    13861 tgcagtagaa cggaagctgc tcctcctcca gaatctcgcc tgcgaagagg ttggtgaggg
    13921 ggacctccgc ggtcgggatc agccacagat cgtcgttctc gcacttgtag agatcgtcgg
    13981 cgaacttcgg gagctgcccc gttccctgca tcgttttgct gttcaccatg aagggcggct
    14041 gcacctcaag gtagccgtgc ttttccgtgt gaaggtccag catgaagttg aggagcgcgc
    14101 gttcaagacg ggctcccaca cctttcagta cggtaaatct gctctgggcc agcgccgcgc
    14161 ccttttcgaa gtccatgata cccgccgcct cgccgatatc ccagtgcgct ttcggttcga
    14221 aagcaaaaga tttgggctct ccccagcggc gaactaccgg attgtcgttc tcgtccttcc
    14281 ctacggggac ggattcgtgc ggacgattgg gaatggagag aagcatgttg ttcatcttct
    14341 cttcaatctc cgcaagcgtg ctatctatat cgcggatcct gtcgccgatg acgcgcatct
    14401 cctccatcag ctcgactgcg tcctcacctt tgctcttcgc cataccgacc ttgcgcgagc
    14461 cctcgttccg cttcgctttc agttcctcgg tctcggagag cagtttccgt ttctcttcgt
    14521 cgagcgcaag aagaggctct atatcgaagt cgttgtttct gttggccagg taggccttca
    14581 cttcatcggc gttggaacga atccatcgga tatcaagcat tctgatcagt ctccttttca
    14641 gtccgcatct gcttcaatat attcgccatc tcgatagcgg ccgccgccgc ctcgaaccct
    14701 ttgttaccgg ccttgcttcc cgaacgggcg acggcctgat ccatcgactc gcacgtcaaa
    14761 acgccgaacg acaccggcac acgctgcgtc atgctgacgg acgcgattcc tttcgacatc
    14821 tccgcgcaga cgtgttcgta gtgcgtcgta tcgcctcgta tcacggcgcc gagcgcgata
    14881 attgcgtcgt acttgccgct cagcgcggcc tctttcgcga tcagggggat ctcccacgct
    14941 cccggaaccc atacgatgtc gatgccgtga tgagacacat catggcgcaa cagcgcgtcc
    15001 ttcgctcctt cgagcagctt ctcgctgaag aagctgttga aacgcgaaac aacgatgcag
    15061 aaccgaagcc ccgcccccgt cagttttcct tcgataaccc tcattcggaa ctctctccct
    15121 tcaggatctc ccttaccgca gaagatcgca tatatgcagc atgtggccga gcttttgctc
    15181 cttggttccg aggtagcgct cgttgaactt gttgggcgga atcaccagcg gtatccgttc
    15241 cgcgatctca agcccgtacc cctgaaggcc gaccaccttc ctcggattgt ttgtcagcag
    15301 cctgatattc tgtaccccaa gatccagaag aatctgcgcc ccgatgccgt aatcgcgaag
    15361 atcgggagca aaaccgagcg cggcgttcgc ctccacggtg tccagcccct tctcctggag
    15421 ctggtacgcc ttcagcttct ccgcgatgcc gatgccgcgc ccctcctgcc gcatgtagag
    15481 gacgacgccg cacccttcct tctccaccat ctccatcgcg gcggagagct gcggaccgca
    15541 gtcgcagcgg agagaaccga agacgtcgcc cgtaaggcac tcggaatgaa cgcgcactaa
    15601 aacgcccatc cgtccgttta cttccccctt cacgagtgcg atatgcaccc gctccgcgtt
    15661 ctcgtccagc acatttttgt acgcgtgcgc cacaaacgaa ccgtgaaccg taggaagctc
    15721 gaccgtcgcg atcttctcaa tcaggcgttc cctcttgctt ctgtacgcta tcaggtcttc
    15781 gatcgaaatg atcttcagcc cgtattccgc ggcaaataca agcagttgcg ggagccgggc
    15841 cattgtgccg tcctcgttca ttatctcgca tattaccccg gcgggcggaa gcccggcgag
    15901 gcggacgaga tccaccgacg cctccgtgtg gccggcgcgc ttcagcacgc cgccctgttt
    15961 cgcttcaaga ggaaacatgt gcccaggacg ataaaaatca tccggcctgg acgacgtatc
    16021 cgcaagcaga cgcgccgtcg cggctcgctc ctcggccgaa atccccgttg tcgtcccctc
    16081 ttttgcgtcg accgaaacga ggaaggccgt tccatgctta tccgtcccct cgcgcaccat
    16141 tggatcaaga cgaagacggc gcgcaaattc cttcgcaaga ggaacgcata caagcccccg
    16201 cgcccttcgc accatgaaat tgatcgcctc ggtggtc
//
LOCUS       DAOPKU010000005        15485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_4003_length_15485_cov_4.932016, whole genome shotgun sequence.
ACCESSION   DAOPKU010000005 DAOPKU010000000
VERSION     DAOPKU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4003_length_15485_cov_4.932016"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..959)
                     /locus_tag="PK849_00330"
     CDS             complement(69..959)
                     /locus_tag="PK849_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPE64595.1"
                     /translation="MERLNIRKLLTQFGELVKAEWSTFWYATLGAVLMSFSIIALIVP
                     YRFAGAGLAGIALLARYVFDVSPAWVLAVGNLLLLGWGWRALSARFVLWTLYVSILSS
                     FAVAFFELFHYPVLANTLLAALLGGVCSGIGMGLVFRVGGSTGGTDVIVAVAKKRWGV
                     DMGMFSFYINTAILFLSWFAVDLEQLLMGGVLLYAESITIDSVLKSFDRRKQVTVITS
                     KMEEVKRFIVEDMEKGATVIRGEGAYTAAERPMIMVVLNRRQAMELKLFTVSVDPNAF
                     IIMADVAEVVGEGFKHWKNL"
     gene            complement(1019..2155)
                     /locus_tag="PK849_00335"
     CDS             complement(1019..2155)
                     /locus_tag="PK849_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE64596.1"
                     /translation="MMTNDPGRRKRLLLVGAGHAHLFSFARIERFLRGGIDVTVISSA
                     PLRFFGTGPEVLGDSDGMKELTVDVRALVERGGGRFLEGRVASVHPQEHCVRTESGEE
                     LFFDALSLNIGSEIPSALLGGSPGRVFAVRSISSLLELRRRILALPPGAKPHLAVAGG
                     GPSGCEAAANVYELSRRHGRSLRITLTTGAAGLLPYFPLKARRLVAAELQKIGVEVSE
                     RRTTLVSGDTLVYSDGSTGDADFLVVATGARPSKVLTESGLEVDDEGAVIVDEYLQSL
                     SAKGVFGGGDCVAFQPMELERSASHALRQGRVLFANLLAYLSGEPMRPFIPKKEPLTI
                     LNLGRTGLLVRKPFVARGRFALLLKRWIDRRIVKEFQSPSEEKS"
     gene            complement(2450..2692)
                     /locus_tag="PK849_00340"
     CDS             complement(2450..2692)
                     /locus_tag="PK849_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64597.1"
                     /translation="MDVGTLRWFFFWCAIFNFFILGGFFLGFIFSGDMIYSFHRKWFT
                     LDRDSFNTVMYFFMGIYKILILVFNLVPWLALLIAG"
     gene            complement(2786..3340)
                     /locus_tag="PK849_00345"
     CDS             complement(2786..3340)
                     /locus_tag="PK849_00345"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HPE64598.1"
                     /translation="MPVVSSFPPVARTDAVICILGSMPGRASLEADQYYAHPRNAFWR
                     LLESVLGIPCSLPYPERLERLKERRTALWDVLGTCARDGSLDSRIEPSSIIPNDFATF
                     FAMHPLIGTVFFNGGKAEAVYRREVLPTLAEPARSIRLLRLPSTSPAHAGMTFEQKKD
                     AWGRLGRVLEENASQGYHERGSDC"
     gene            complement(3377..4681)
                     /locus_tag="PK849_00350"
     CDS             complement(3377..4681)
                     /locus_tag="PK849_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HPE64599.1"
                     /translation="MEFIVFLVIAALLVRFIKSYFGAPKRDEILVIPGDAENYRSGSK
                     NASSQWIEPGQSFICRGYSIDDGMVYVGERMRDFTGANEGCLINPELDVAPEGTPSLP
                     VQQIQPRYETMSPEQRGAFLRWLAEGRFGRADPAWPFLFFYGIERRLFVDGPRGYVSH
                     QERNELVLEVRRLLHVYGEHRSFRGYCKNLLATEWVLKGDYAEIPEWVDFTDRFCAEA
                     FNSIISLYVSRDQPIPGEVALQWLFLHPEHISGTLSRREPEKFEHLFLAGYKEKFGEG
                     ILIHKNRSPLNLIYKGANPSLGNGVKLTVPSLPDPFLLATPIKKLDLLAEECIKAMET
                     DDTLEFKGQVFEADGVDPLESLDDIHSEFMARLATKMLWPRADVLAVCDALSIAPEPS
                     FELLNSWGRVAGGLPLIDDGDPVFVDIPLLQEMLLRENADAQ"
     gene            complement(4790..5824)
                     /locus_tag="PK849_00355"
     CDS             complement(4790..5824)
                     /locus_tag="PK849_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64600.1"
                     /translation="MIIGRDDLRSAAKKGILSEEQADSLWDFLSEEMREVPSFRMAHI
                     LYYLGGLIAIGAMTLFMNRGWELFGGWGIFAISLAYALVGLFFLAYFMKKPGLSIPAG
                     ILGAFVAVMVPLAVYGLQNALGYWEGNRIYREYHVYIDWRWLSMELATLAVAVGLLWR
                     WKLPFMLMPVALTLWYMSMDLAPFLFKNEDLTWELRRTVSLWFGLAVLCFAVFVDFRT
                     RSRKDYAFWLYLCGLAAFWGGLSLMDSDSELGKFVYFCINIFLIAVGALLSRRAFAVF
                     GGFGAAGYIGHLTWRVFSDSVVFPFVLSFAGFFVIWLGIRWQKKEKQITALLRSFLSP
                     EMRSFLERNE"
     gene            6025..6450
                     /locus_tag="PK849_00360"
     CDS             6025..6450
                     /locus_tag="PK849_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPE64601.1"
                     /translation="MIAKKGSKVKVHYTGTLKNGDVFDSSEGRSPLEFTVGAGQMIPG
                     FDKGVEGMNAGEKKTITLPPKEAYGEKRDDMFFRVSRDVLPEGYTPSIGDPLRVATDD
                     GGVLNVFIAEMDEDGITLDGNHRLAGEELTFEVTMVEIA"
     gene            6635..7945
                     /locus_tag="PK849_00365"
     CDS             6635..7945
                     /locus_tag="PK849_00365"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPE64602.1"
                     /translation="MSREQRAEDSWPLLAAAHAAASLDRPLICLFCFAPSFAHAQKRH
                     YAFLIQGLKETSRQLRKYNTQMKILYGKPGPTIAALTATLDAALVFIDFDPFPEKQPW
                     RKEFLDRVSTTVYEVDGHNIIPAWIVSPKKEYSAATFRRKVHPLLDSYLEEYPLFPEL
                     PPRESIETPLFLSLEDAAARVATETAEAPLSPYSPGSSAARAAMRRFFSERLNDYDTR
                     RNNPALSALSDLSPWLHFGQLSPQRVALEALKTPQSPAREAFLEELIVRRELADNFCF
                     YEPMAGTYDGLPEWGRKTLALHAGDRRPRLYTMAELEEARTGDPLWNAAQKEMVWKGK
                     MHGWARMYWAKKILEWTETPEEAFERVLILNDRYELDGRDPSGVVGVSWAIGGLHDRP
                     WAERSVFGMVRYMNDKGAARKFRVADYIESVNAFISNRTGENLS"
     gene            complement(8165..8755)
                     /locus_tag="PK849_00370"
     CDS             complement(8165..8755)
                     /locus_tag="PK849_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPE64603.1"
                     /translation="MRYKGWILAGFFAGLTVVGTFLRIPFPLVPLTLQVLMVLLAGFT
                     LGPKYGMLSQVIYLFIGLAGLPVFSGGGGIGTVFSPTFGYLLSYPAAAWIVGRISSKE
                     GANAIRFVVASCAGIAFIYLLGASVLYLNLKFLAGKSIGVYQVVKIGVLPFLLPDLLK
                     GGVAALFALKIRPLLGTSGAELRRAPLHGRLTRYKP"
     gene            complement(10249..10869)
                     /locus_tag="PK849_00375"
     CDS             complement(10249..10869)
                     /locus_tag="PK849_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPE64604.1"
                     /translation="MQVDYKEPASETTVEVKINKSRFLGHVRICRSEEEVRMKLKEVC
                     DLHKQATHNCWAYRIGEESYREYFSDDGEPAGTAGKPILGAISRLDLTNALIVVTRYF
                     GGIKLGVRGLIEAYGSTATNALNASGMAECTIKSQYQIVAPYDMVKTLHRLLFSLGAV
                     EKDVLFDYGERVSILCSLPRSSGKDFESALDELLNMSRIHSWNGVQ"
     gene            complement(10869..11348)
                     /locus_tag="PK849_00380"
     CDS             complement(10869..11348)
                     /locus_tag="PK849_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPE64605.1"
                     /translation="MENIFAPWRMAYIAVSKDQGGCIFCDFPKEDNDEARMILFRGET
                     CFVIFNAFPYNPGHLMVAPYRHTAEYELLTDEELLEMHRLAGKCIVVLRKTMNPQGFN
                     LGVNLGKVAGAGFDGHLHLHIVPRWNGDTNFMPVFADVRVVAEELAETYRKMKEAWD"
     gene            complement(11659..12549)
                     /locus_tag="PK849_00385"
     CDS             complement(11659..12549)
                     /locus_tag="PK849_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPE64606.1"
                     /translation="MAKGRGLGKGLASLLPAAQTLGEGEPEITMLSMDRILANPFQPR
                     KEINENTLVELADSIRAHGVIQPLIVRRNEDGDYWIVAGERRWRAAKIVGLSEVPVRV
                     IDGNEMEMREITLIENIQREDLSPVDTALALEELLNVYDITQDELAASIGWSRTSVTN
                     KLRLLQLPEEVKTLLSNNELSEGHCRALLPLESQDEIAKLASDAVNAGLSVRQVEDIV
                     KRKNAHSPKEKRPKDVFALPESISQKARSLGFSIRITGKKSKMKLHIEGVHEELVRDV
                     LTYLEENAEKYFPGNTPDEQ"
     gene            complement(12528..13298)
                     /locus_tag="PK849_00390"
     CDS             complement(12528..13298)
                     /locus_tag="PK849_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE64607.1"
                     /translation="MITLAVSNQKGGVGKTTTCINLAAELGRKGYSVLSVDIDPQANC
                     TSGLGVALPPKAPSLYNVLLGDVPPQEAILQTKWKGVSMLPATLDLAGAEIELASSIS
                     RETKMRRVLSRIEGFDIAILDCPPSLGMLTVNALVAADKLVIPIQCEYFALEGLGQLS
                     RTISLVKDGLNQNLGIDGILLTMHDSRTRLSIDVADEVRRQFGDVVFKTFIPRNVKLA
                     EAPSYAEPICYYDPSCSGAKAYEEFAEEVSTLWLKEED"
     gene            complement(13306..13980)
                     /gene="rsmG"
                     /locus_tag="PK849_00395"
     CDS             complement(13306..13980)
                     /gene="rsmG"
                     /locus_tag="PK849_00395"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPE64608.1"
                     /translation="MTESVESLVRVHEKKLREYVSLLSSYEGKIRLTGPSDPEVLWNE
                     HIVDCLHSVPYLPESGRTLDVGSGGGLPGLVWALCRPDLQVTLLDSVRKKCGALAEMA
                     SALQLENVQVVCARCEEYAVKEREVFQCAAARAVAAADVLLEYLAPFVTVSGRVLAFK
                     GPLYVEEIKPMEGKWARLGLSAPEVHTYTAGEKERYLLLWTKQTSVPQRFPRKIGMAE
                     KTKWWR"
     gene            14080..14838
                     /locus_tag="PK849_00400"
     CDS             14080..14838
                     /locus_tag="PK849_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPE64609.1"
                     /translation="MRLYDSILQEVLKAPDGAVDNLIIGWKGSFLSLSDGRWGVGSTP
                     PEGGEPMSPREEHTRNILMSGAHSLARLLVSPYPQEFSAASASLAALAPAPEGGEPLE
                     FLLQLPGRDRVALLAPDPWVTDFLRDWNWKISIFDDSRRGLGILPEWTTSQHLHSSAV
                     LWINAETLRNRRILSLMPLLKGKRGVILQGPGIPFLPSVFREAGITHLVLPAAEEAAA
                     PETRRYIAAGGTPWTCPSLRWRVFPLLYTDEKNR"
     gene            14835..>15485
                     /locus_tag="PK849_00405"
     CDS             14835..>15485
                     /locus_tag="PK849_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPE64610.1"
                     /translation="MRRIALFLLLFALFFVLFLFFRAVPFQRDEQPFPPPGVRVFDGD
                     TAELPSGGGVARVRYLLVDTPELHHPSRPAEELGEEARLLNSRLLASGPLRLEYDSER
                     TDRYGRTLAYVFAGGEGSELFLNEILVREGLAQPLIIPPNRRYAGRILKAFEEARAGQ
                     RGLWNRAEGRVFTSAQVWSEAPLLAGSFLTLFMTIEKAELKGRRILVTRPKTGNTRM"
BASE COUNT         3822 a         4333 c         3926 g         3404 t
ORIGIN      
        1 aaaaaagccg ggaatcttcc cggcttcgta aggcagcatt cttcccgcgg ttcttccgcg
       61 caggaaggtt agaggttctt ccagtgcttg aacccctcgc ccaccacctc ggcgacgtcg
      121 gccatgatga tgaacgcgtt cgggtccacg gagacggtaa agagtttcag ctccatcgcc
      181 tgccgccggt tgagcaccac catgatcatc gggcgctccg cagccgtgta ggctccttcg
      241 cccctgataa ccgtggctcc tttctccatg tcctcgacga taaagcgttt gacctcctcc
      301 atcttcgatg tgatgacggt aacctgcttt ctccggtcga aggacttgag cacgctgtcg
      361 atggtgatgc tctccgcgta gagcagcact cctcccatga ggagctgttc gaggtccacg
      421 gcgaaccatg aaaggaagag aatcgcagta tttatataga aggaaaacat tcccatgtcg
      481 acgccccagc gcttcttcgc aacggcgacg atgacgtcgg ttccgccggt ggagcctccg
      541 acgcggaaga ccagccccat gccgattccg ctgcacaccc cgcccagcag cgcagcaagg
      601 agggtgttgg ccagcacggg gtagtggaag agttcaaaaa aggcgacggc gaacgaggag
      661 agtatcgaga cgtagagcgt ccagaggaca aagcgcgcgg agagcgccct ccacccccag
      721 cccagcagca gcaggttgcc gacggcgagc acccacgcgg gggagacatc gaagacgtac
      781 cgtgcgagca gagcgattcc tgcgaggccg gcgcccgcga aacggtacgg aacgatgagg
      841 gcgatgatgg agaaactcat cagcaccgcg ccaagcgttg cgtaccagaa ggtgctccac
      901 tccgctttga ccagttcgcc gaactgcgtc aagagctttc gtatgttcaa acgctccatg
      961 attcattcct ttcaactgag agaaattgag ggtacgcaaa aggctttcat cgttcgtctc
     1021 acgatttttc ctcggaggga ctctgaaact ccttcacaat acggcggtcg atccatcgct
     1081 tcaacagaag cgcgaagcga cctctggcga cgaacggctt cctgacaagc agccccgttc
     1141 ttcccagatt caggatcgtg agcggctctt tcttcggaat aaacggacgc ataggctccc
     1201 ctgagaggta cgccaaaaga ttcgcgaaga gcacccgccc ctgccggagg gcatgggaag
     1261 cggaacgctc cagctccatg ggctgaaagg caacgcagtc tcctccgccg aacacgccct
     1321 tcgccgaaag cgactggagg tactcgtcga caatcacggc gccctcgtcg tcaacctcaa
     1381 gccctgattc cgtcagcact ttcgacggac gcgcgcccgt tgcgaccacc agaaaatcgg
     1441 cgtctccggt cgaaccgtcc gagtagacga gcgtatcgcc ggatacgagc gtggtgcgcc
     1501 gttccgaaac ctcgacccca atcttctgga gctcggccgc cacaagccgg cgcgctttca
     1561 gggggaagta cggcaatagc cctgccgctc ccgtggtgag cgtgatacgg agagagcgtc
     1621 cgtgccgcct gctcaattcg tacacgtttg cggccgcttc gcatcccgag ggacctcctc
     1681 ctgcgacagc aaggtgcggc ttcgcaccgg gaggcaaggc aagtatccgc cgtcgcagct
     1741 ccagaagcga ggatatggac cttacggcaa agacgcgccc cggcgagccg ccgagaagag
     1801 cggacggaat ttcgctgccg atatttaaag agagcgcgtc aaaaaaaagt tcctctccgc
     1861 tctccgttcg cacacagtgt tcctgcggat gtacggaagc gaccctcccc tccaggaagc
     1921 gccctccccc gcgctcgaca agggcgcgca catctacggt caattccttc atcccatcgg
     1981 aatcgccaag tacttcaggc cccgtcccaa aaaaccggag gggcgcggaa gagatcacag
     2041 tcacatcaat cccgccgcgg agaaaccgtt ctatgcgcgc gaagctgaaa aggtgcgcgt
     2101 gcccggcgcc tacaaggagc aggcgttttc ttctccccgg atcattcgtc atcatgcgca
     2161 acctcctttc ctgaacagac tggtttcaaa ccatgaaaag cattataaac catgagagca
     2221 ggccgtatct tcagacttct tgagttctgc cgatgactgt ttactgggaa cggaaccttc
     2281 ttggcacgca ggagacaccc ggcacaaagg gtggggtgca gacggatcaa gggcgcagtc
     2341 gacgtgaaat cgcgctttga gggaataaag gacgaacgga aaggggtttt caatagaagg
     2401 gcgcgtccca gggcgaggga aaaacagcta tggaacgcgc ccctcgtaat tagccggcga
     2461 tcagaagcgc gagccaggga accaaattga agaccagtat caggattttg tagattccca
     2521 taaagaagta catcaccgtg ttaaaagaat ccctgtccag cgtgaaccac ttgcggtgaa
     2581 aagaatagat catgtcgccg gaaaaaatga atccgagaaa gaagcctcca agaataaaga
     2641 aattaaaaat ggcgcaccag aaaaagaacc aacgcagtgt accgacatcc attacaaacc
     2701 ctccctttgt tatgatccat cgaatcagga gaccacctgc ggtcacggag gtactcccgg
     2761 gaagattgat atgctcactc tctcctcagc aatcgctccc ccgctcatgg tacccttgac
     2821 tggcgttttc ttcaaggact ctcccgaggc ggccccacgc atccttcttc tgctcgaagg
     2881 tcattcctgc atgcgcggga cttgtagagg ggaggcggag aagtcgaatg gatcgggcag
     2941 gttcggcaag agtcgggagt acttctctgc gatagaccgc ctcagccttt ccgccgttga
     3001 aaaaaacagt tcctataaga ggatgcatgg cgaaaaaagt cgcaaaatcg ttgggaatga
     3061 tactggacgg ttcgatacgt gaatcaaggc tcccgtcgcg agcgcacgtc ccaaggacgt
     3121 cccagagcgc tgtacgtcgt tctttcagcc gttcaagcct ctccggatat ggcaacgagc
     3181 agggaatacc cagtacggac tcaagaagac gccagaaggc atttcgaggg tgcgcatagt
     3241 actggtcggc ttcaagcgac gctctgccgg gcatactgcc gaggatacag atcacggcgt
     3301 ccgtccgggc gaccgggggg aaacttgaaa ctaccggcac aaaataacct tcctctcttg
     3361 aggcgctccc ggcacgctac tgagcatctg cattctcccg aagcaacatc tcctgcaaca
     3421 aagggatatc cacaaaaacc ggatccccgt cgtcaatcag gggcagtccc ccggctaccc
     3481 gcccccacga gttcagcagc tcgaaggacg gttccggcgc aatggaaagg gcgtcgcaga
     3541 ccgccagaac atccgctctc ggccagagca ttttcgtcgc caggcgggcc ataaattcgc
     3601 tgtggatatc gtccagagac tcgagaggat cgacgccgtc ggcctcgaac acctgtcctt
     3661 taaattcaag agtgtcatcg gtctccatcg cctttatgca ctcttcggca aggaggtcga
     3721 gtttctttat aggcgttgca agaagaaagg gatccggtag agaaggaact gtgagcttga
     3781 caccgtttcc cagcgaaggg ttcgctcctt tatagataag gttcaagggc gaacggttct
     3841 tatggataag gatgccttcg ccgaactttt ccttgtatcc tgcgaggaaa agatgttcga
     3901 acttttccgg ttcgcggcgc gaaagcgtcc ccgaaatatg ttcaggatgc agaaagagcc
     3961 attgaagcgc cacttcgccg ggaatcggtt gatcccgcga aacgtagagc gagatgatgg
     4021 agttgaacgc ctcggcgcaa aaacgatcgg tgaagtcgac ccattcggga atttccgcat
     4081 aatccccttt gaggacccat tcggttgcaa ggaggttttt acagtatccc cggaatgaac
     4141 ggtgctcgcc gtagacatgc agcagacgcc gcacttccag gacaagctcg ttgcgttcct
     4201 ggtgagagac gtaccctctc ggtccgtcca caaagagccg ccgttcgata ccgtagaaga
     4261 agagaaaggg ccatgcgggg tccgcccggc cgaatcgccc ttctgcgagc cagcgcagaa
     4321 aggccccgcg ctgctcgggg ctcatcgtct cgtaacgggg ctgtatctgc tgtaccggta
     4381 acgagggggt accctcagga gcaacgtcca gttccggatt gatgagacac ccttcgttcg
     4441 cgccggtaaa atcccgcatt ctttcaccga catacaccat tccatcatca atcgaatacc
     4501 ctctgcaaat gaaagactgt cctggctcga tccactggga agaggcattc ttcgaaccgc
     4561 ttctgtagtt ttccgcgtct cccggaatga ccagaatttc gtcgcgcttc ggagctccaa
     4621 agtaggattt gataaagcga accagaagtg cggcgatcac aagaaagaca ataaactcca
     4681 cgtttttccc tcttttcagc agggcttcaa agcaacaggc ccttcactag atttccgcgg
     4741 attccttcgc ggccggcgcc tctccgaatc gtcacgctct cggagggaat cactcattcc
     4801 tctcaagaaa actccgcatt tcgggggaga gaaaggagcg cagcagcgcc gtaatctgtt
     4861 tttccttctt ctgccagcgt attccgagcc agatgacaaa aaaaccggca aacgaaagaa
     4921 cgaacggaaa gactacgctg tcgctgaaca ctctccatgt taaatgacca atatacccag
     4981 cggccccaaa tccgccgaag acggcaaagg ctcttcgtga gagaagggct cctacggcaa
     5041 tcagaaagat gttgatgcag aagtagacaa acttccctag ttcgctgtcc gagtccatca
     5101 gagagagccc gccccagaaa gcggcgaggc cgcacagata gagccaaaac gcataatcct
     5161 ttcgtgaacg cgtacggaaa tcgacgaata ccgcaaaaca gagcactgcg aggccaaacc
     5221 agagcgaaac cgtccgtcgc agttcccaag taagatcctc attcttgaag aggaagggcg
     5281 ccaggtccat gctcatgtac cagagggtca gcgcaaccgg catgagcatg aagggaagct
     5341 tccagcgcca taaaagtccc accgcgaccg caagagttgc caattccatg gacagccagc
     5401 gccagtcgat gtacacgtgg tactccctgt atattctgtt cccctcccaa taccccagcg
     5461 cgttctgaag gccgtatacc gcaagcggca ccatcaccgc cacaaatgca ccgagaatac
     5521 ccgcgggaat ggagaggccg ggttttttca tgaaatacgc cagaaagaag agacctacaa
     5581 gcgcataggc aagtgagata gcaaagatgc cccatcctcc gaaaagctcc cacccccgat
     5641 tcatgaagag cgtcatcgca ccgatcgcaa tcagtccgcc aagatagtag agaatatgcg
     5701 ccatgcgaaa agaggggact tctcgcatct cttcggaaag aaaatcccat agggaatccg
     5761 cctgttcttc ggagagaatc ccttttttcg ctgcacttct caaatcatct ctgccgataa
     5821 tcatctctca ctcacctccc gggaaagatt tgccgtcata ttcgtattct acctcataat
     5881 cggacgcttt ccagagcttc ttcaaaatac ttcgacggta gatttttttc tccggatgaa
     5941 atagactggt tgaagagagc gcatcggggt tctttcatcc gggaaatgcg cgcttccggt
     6001 tcggaccgga gaaaggagca acgaatgatt gcgaagaaag gttcaaaagt gaaagtccac
     6061 tatacgggca ctctgaaaaa cggggatgtc ttcgactcct cagaaggacg atcccctttg
     6121 gagttcaccg ttggcgccgg acagatgatc cccgggtttg acaagggggt tgaagggatg
     6181 aacgcaggag agaaaaagac catcacactc ccccccaaag aagcctacgg ggagaagaga
     6241 gacgacatgt tcttcagagt ctcgcgcgac gtcctcccgg aaggatacac gccctccatc
     6301 ggcgatcctc tcagagtagc gacagacgac ggcggcgtac tcaacgtctt tatcgccgag
     6361 atggacgagg atggaatcac tctcgacggg aatcaccgcc ttgccggcga agaactcaca
     6421 tttgaagtaa caatggtaga aatagcctga gcaacgccac caaataaaag aggaggccgc
     6481 agcgaggaga cacgccgcgc ctcctctttt atttcccggg aaatacaact gaaaggagag
     6541 atcaaatatg ccctttcaat tttctgaacc attttcacga agagcacatc tgcttcaaaa
     6601 cggaagtcaa ggcaaaggtc ctgttctcta ttggatgagc cgcgagcagc gcgcagaaga
     6661 cagctggcct ctcctcgccg cggcgcacgc ggctgcttcg cttgaccgcc ctctcatctg
     6721 ccttttctgt ttcgctcctt ccttcgccca cgctcagaag cgacactatg ccttccttat
     6781 acaaggtcta aaagaaacat cccgacaact acgcaaatat aacacgcaaa tgaaaatact
     6841 atacggaaaa ccgggcccaa ctatcgctgc actcacggcg acgctcgatg cagcgcttgt
     6901 cttcatcgat ttcgacccgt tcccggagaa gcagccgtgg agaaaggaat tcctcgacag
     6961 agtttcgaca acggtctacg aggtcgacgg gcacaacata atcccggcgt ggatcgtatc
     7021 gccgaagaag gagtacagcg ccgcgacatt tcgcagaaaa gtacaccctc tgcttgattc
     7081 ctatctcgaa gagtaccctc tgtttccgga actccctccg cgagagagta tagaaacgcc
     7141 gctttttctc tctcttgaag atgctgccgc ccgagtcgca acagaaaccg cagaagcgcc
     7201 cctttcgcct tactcgcccg gctcttccgc tgcccgcgct gccatgcgcc ggttcttttc
     7261 agaaagattg aatgactacg acactcgaag aaacaatccg gcgctttctg ccctctccga
     7321 tctttcgccg tggctccact tcggccagct ctccccgcaa agagtcgcac tggaggcgct
     7381 gaaaacaccc caatcccccg cgagggaagc ctttctcgaa gaactcatag tccgtcgaga
     7441 gctggcagat aatttctgtt tttatgagcc aatggccgga acctacgatg gactccctga
     7501 atggggccgg aaaacgctcg cgcttcatgc gggcgaccgg agacctcgtc tttacacaat
     7561 ggcagagctt gaagaagccc gaaccggcga tcctctctgg aacgctgcgc aaaaagagat
     7621 ggtctggaag ggaaagatgc acgggtgggc gagaatgtac tgggcgaaga aaatcctcga
     7681 atggacggag accccggaag aggcctttga acgtgtgctc attctcaacg accggtacga
     7741 gcttgacgga cgcgacccca gcggcgtcgt aggcgtttca tgggcaatcg gcggtcttca
     7801 cgacagacct tgggctgaac gctctgtttt cgggatggta cgctatatga acgacaaggg
     7861 agcggcgaga aagtttcgtg tagccgacta tatcgagagc gtcaacgcgt ttatatcgaa
     7921 ccgaacggga gagaatctca gctaagatca gtattagtca acgtctatga acactacttt
     7981 gtttaaaatt ctgctcatcg gaagcatgta gatcgtgata ttggagacac gctcctcttt
     8041 ctgcaacgaa gatgagggcg gcccccccca tttcaaacat aacacaatgc cgccggagaa
     8101 aggcctgagt cctctcaact ggcggcattg tcattcgttc attgaacacc gggattccgg
     8161 tctgttacgg cttgtatcgt gtaagcctcc cgtgcagagg cgctctccgc aactctgcac
     8221 cgcttgtacc aagaagcgga cgtattttca aagcaaacag cgcggccact ccccccttga
     8281 gaagatcagg gagtaagaag ggcagaacac cgatcttcac aacctggtac acaccgatgc
     8341 tctttccggc caaaaacttc aggttgagat agagcactga agcgccgagc aaataaataa
     8401 acgctatccc cgcacatgag gcaacaacga aacggatcgc gttcgctcct tccttggaag
     8461 agatccggcc aacgatccat gcggccgcag gatatgagag aaggtagcca aacgtcggag
     8521 aaaagaccgt tccgatccct cccccgccgc tgaaaaccgg tagtcccgca agtccgataa
     8581 acaggtagat gacctgtgat agcatgccgt atttcggtcc cagcgtaaaa ccggcgagaa
     8641 gcaccattaa tacctgaagc gtcagcggaa ccagagggaa aggaatacga agaaacgttc
     8701 caacaactgt aaggccggcg aaaaaaccgg caagaatcca ccctttgtat ctcatcgagc
     8761 accccctcag ctatgtcacc cggatatttt agtggttgac atagcttttt gcaacgtatt
     8821 tccgcctcct cgtctgcttc tctcttaaag gaagccatat tccgttgtca gcgaacaggc
     8881 gaagtatatg ttctctggga aaaatctttc acgttcctgt aaacagtctc tctgatttct
     8941 agtcttcgcg agaaagaaac accataattg cgtatgaaga agctgatcta tactgaccta
     9001 caccctccaa ataggtctct ttgtatcgaa aggatgttcc aacagttgac aacgttcacc
     9061 ctcaccagtc tgaaggcaca cttccggaat agcgtacaac cgctaatttg cagcgcatgc
     9121 aatcacctgt aacaattcac aaagtctcca actacaaaga ggcccccaac gtgcctctca
     9181 aagcagcgca ctataacaaa gatacagagc attttccgcg atgtcttggg tacagccgat
     9241 cccggccgcg catgcaattg atacaatcgg ccgcatgaaa ggataaatgg gttttcggac
     9301 tcttttgtct ttcgaaccac acattgaatg ctatttagcg tgcagaatgt cacttcaata
     9361 gaggatccac gtgacaggaa gaaagaagcc aaatggacgt gtacaagaat tccaccacta
     9421 cttctcaata cgatgagtaa ggaacagaga tgcacgcatt tcgaccagac acgaaatgtg
     9481 gacgtgtggc gcgttgaatc agactgtctc attgactcat aacatcatca accagaaaga
     9541 gaagaaacga agattttttc taagagatcc actttggttc tatcagaact gtttcattca
     9601 acaagaagag tttcgaaagg gaacaaacca ggagatttcg atgaaaagaa cgaaagaatt
     9661 ttggctctta atcaaaataa atacgacgat gcacttcgga catccggaag aaggcacacg
     9721 aaagtggcat tgtggttgaa ttatgtttag tggtgagctc gagtatcgaa ggcgaaggga
     9781 acaactaaag aaatctactt tttacgagtt cgaggaagga gtttgttttt ccgtgattcc
     9841 attccaaata tggactacaa ttcctggaac tcattcataa atgaaaacgg ttcgtattga
     9901 gactctttgc tgagtctcaa tacgaaaagg agaaagcgtt gataaccgga gctgctgtca
     9961 gatgatcaat tttcggctgt cgttcgctca aaaagtgatc aatcttagtg taccaaaacc
    10021 aagcgatata tgaaaaagac tcatgtgttg tagaaagtta ctctccagag aattcgcggc
    10081 gaaaaatgtg attcttcaac aaaggaaacc cagaatactg tcgagtcaga ctcgtaggaa
    10141 ctcgttccaa tcgagctcga tgaagaaaga agaccgttga atcagatctt ctcaaagatt
    10201 gaaagggtgg caaaaaaaac ggaacggcgc ccggaagaat gaagaagtct actgaacacc
    10261 gttccatgaa tgtattctgc tcatgttcaa gagctcatca agcgcgcttt caaaatcctt
    10321 tccggaggat cgaggcagcg aacaaagaat actgacacgc tctccgtagt cgaagagaac
    10381 atctttctcg actgcgccaa gtgaaaataa cagccggtgc aatgttttta ccatatcgta
    10441 cggagcaact atctggtact gactctttat cgtacattcc gccataccgg aggcattcag
    10501 cgcattcgtc gcagtggagc cgtacgcttc gattaaaccg cgaacaccaa gctttatgcc
    10561 tccgaaatat cgagtgacta caataagagc atttgtcaga tcaagtcgtg aaattgctcc
    10621 aaggatcggt ttccccgctg tccctgcggg ttcgccgtca tcggaaaaat actctctata
    10681 tgactcctcg ccgatcctgt atgcccagca attatgcgtt gcctgcttat gcagatcgca
    10741 cacctccttg agcttcatcc gaacttcttc ctcggatctg caaatacgca catggcccaa
    10801 gaatctggat ttgttgattt ttacttctac cgtagtttcc gaagctggtt ctttgtaatc
    10861 aacctgcatc aatcccaagc ttccttcatc tttctgtatg tttctgccag ttcctccgcg
    10921 actactcgta catctgcaaa tacaggcata aaattcgtat ctccgttcca tcgaggaaca
    10981 atgtgaagat ggagatgacc gtcaaaacca gcccccgcca cctttccaag attcacgcca
    11041 agattgaaac cctgcgggtt catcgtcttt cggagcacca cgatgcactt tcccgcaaga
    11101 cgatgcattt cgagcagttc ttcgtcggtc aacaattcat actcggctgt atgcctatag
    11161 ggggcgacca taaggtgccc cggattatat ggaaaagcat tgaaaataac aaagcacgtc
    11221 tcccctctga aaagaatcat tcgcgcctca tcgttatcct cttttggaaa atcacaaaaa
    11281 atacacccgc cttggtcctt tgaaacagca atatacgcca ttctccacgg cgcaaaaata
    11341 ttctccacag cgcaaccccc ttcaacaccc aatgtttcac gtgaaacata taaagcaaac
    11401 atttacatta tattgtttgg tttttatacc ctaccgctgt attctatttg ttgattttat
    11461 gaattaattc ccttatgacg aaatacattt ggtgtgttcc gactattgct ccaaccagta
    11521 tgagtgccgg gtgtgtccag gtagggtatc cgttatctac caatcttctt cccaaatagt
    11581 aaccgagcaa aatatatcct accacgagga gtgctgtgga tactaccttt ccgaaaagga
    11641 tcagatctgc gtagtttttc attgttcgtc tggagtattt cccgggaaat acttctccgc
    11701 gttctcttca agatatgtga gaacatcccg gacaagctct tcgtgaactc cttcgatatg
    11761 cagcttcatc tttgatttct tccccgtgat tcgtattgaa aatccaagag atcgcgcttt
    11821 ttgggagatg ctttcaggaa gcgcaaagac atcctttggt cttttttctt tcggagagtg
    11881 ggcgttcttt cgttttacga tgtcctcaac ctgacgcaca gataacccgg cgttcactgc
    11941 gtctgaggca agtttcgcaa tctcatcctg agactccagc gggagaagcg ctctacagtg
    12001 accctcggag agctcgttgt tcgagaggag cgttttaacc tcttcaggta actgaagaag
    12061 gcggagctta tttgtgacgg acgttctgct ccatccaatg cttgcggcaa gttcatcctg
    12121 tgtgatgtcg tagacgttca gaagttcttc gagtgcgagc gcagtgtcca ctggtgagag
    12181 atcttcgcgt tgtatgtttt cgatgagtgt gatttcacgc atctccatct cgtttccatc
    12241 gatcacgcgc acaggaactt cgcttaagcc tacgatcttc gcggctctcc atcgtctctc
    12301 tccggcgact atccagtagt cgccgtcttc atttctccgg acgatcaacg gttgtattac
    12361 tccgtgcgcc ctgatggagt cggcaagttc aaccagagtg ttttcgttga tctcttttct
    12421 tggctggaac gggttggcga gaattcgatc catcgaaagc attgtgattt ccggttctcc
    12481 ctccccgagc gtttgcgccg ccgggagcag cgacgcaaga ccctttccta gtcctcttcc
    12541 tttagccaca gcgtcgacac ctcctccgcg aactcttcat aggcttttgc acctgagcat
    12601 gaggggtcgt aatagcagat tggctcagcg tagcttggag cttccgccag tttgacattc
    12661 ctcgggatga atgttttgaa gacgacgtcg ccgaactgcc tccggacttc atcagccaca
    12721 tcgatagaaa gacgcgtcct tgagtcgtgc atggtcaaga gaattccgtc gatgcccagg
    12781 ttttgattca gtccgtcttt tacgagcgat atcgtcctgg agagttgccc taatccttcg
    12841 agtgcaaaat actcgcactg tataggaatg accagcttat cggcggcgac cagcgcgttg
    12901 accgtcagca tgccgagtga aggcggacag tcgagaattg cgatatcgaa gccctcaatt
    12961 cgggagagca cacgacgcat cttcgtctct ctgctgatag aactcgctag ctctatctcc
    13021 gcccccgcca ggtcgagcgt cgccggaagc attgaaacac ccttccactt ggtttgaagg
    13081 attgcctcct gcggcggtac gtcgccaagg aggacgttat acaagctggg tgctttcgga
    13141 ggcaaggcaa cgccgagccc gctcgtacag tttgcctgcg ggtcgatatc gaccgagagg
    13201 acggaatacc ctttgcgtcc aagctccgca gcgagattga tgcatgttgt ggtctttccc
    13261 actccgcctt tctgattcga tactgcaaga gtaatcactc ccgcatcacc tccaccattt
    13321 tgttttttcc gccatgccta tcttccgtgg aaatcgttga ggaacagacg tttgttttgt
    13381 ccagagaaga aggtagcgct ctttttcacc ggctgtatag gtatgcactt cgggagcgga
    13441 gagtcctagg cgcgcccatt ttccttccat cggcttgatc tcctctacgt agagcggtcc
    13501 tttgaatgca agcacacgtc ctgaaacggt cacaaaggga gcaagatact ccaggagcac
    13561 atccgctgcg gcaacggcac gggctgctgc acattgaaaa acctcccgtt ccttcacggc
    13621 gtattcttca cagcgagcgc agacaacctg aacattctcc agctgtaacg cggaggccat
    13681 ctcggcaagc gcgccgcact ttttgcgaac gctgtcaagc agcgtcacct ggaggtcggg
    13741 gcggcagaga gcccacacaa gaccgggaag acctcctccc gaccccacgt cgagcgttct
    13801 tcccgattcc ggtagatacg gaacggagtg gaggcaatca acgatatgtt cattccagag
    13861 gacttcgggg tcgctcggtc ccgtcaatcg aattttgcct tcgtacgagg acaggagcga
    13921 tacatactcc ctcagcttct tctcatgtac acgcaccagc gattcaacac tttctgtcaa
    13981 tacgcttctc ctttcgacgt tcagaatttg agttttcaaa gagtagcgct tctgcttctc
    14041 tcctcgtatg cgttacaatg gtcggcgagg aggagcgata tgcgcctgta tgattccatt
    14101 ctacaagaag tcctgaaagc tcccgacgga gccgtcgaca atttgatcat cggctggaag
    14161 gggagttttc tctcgctttc ggacggccga tggggcgtcg gttccactcc gcccgaggga
    14221 ggcgagccga tgtccccccg ggaagagcat acgcgcaaca ttctcatgtc cggcgcccat
    14281 agtctcgccc ggctcctcgt ctccccgtat ccgcaggagt tttccgctgc gtcggcctcg
    14341 ctcgccgcgc tcgccccggc gcctgagggg ggcgaacctt tggaattcct tctgcagctc
    14401 cccgggaggg accgggtcgc ccttcttgcc cctgacccct gggtgacaga ctttttgcgc
    14461 gactggaact ggaagatttc catcttcgac gactcccggc gcggtcttgg tattcttccc
    14521 gagtggacga catcgcagca cctgcattcg agcgccgtac tctggataaa cgcagaaacg
    14581 ctgcgcaacc ggagaatcct ctcgcttatg ccccttctga aagggaagag aggagtgatc
    14641 ctccaggggc caggaatacc gtttcttccc tcggttttca gagaggcggg cattacgcac
    14701 cttgtcctcc ccgccgcgga ggaagcggct gctccggaaa cgcggcgata catcgcggcg
    14761 ggcggtactc cgtggacctg cccttccctt cgctggagag tcttccctct attatatacg
    14821 gatgagaaaa acagatgaga cgaatcgcct tgttccttct cctttttgcg ctgttctttg
    14881 ttctctttct cttttttcgc gccgttcctt ttcagcgaga cgagcagcct tttccgcctc
    14941 cgggggtcag ggtcttcgac ggagataccg cggagcttcc ttcgggaggg ggcgtcgcac
    15001 gagtacgata ccttctggtg gatacgccgg aactgcatca cccgtcccgt ccggcagaag
    15061 agcttggaga ggaagcgcgc ctgctgaaca gcagactgct tgcaagcgga cctcttcgtt
    15121 tggagtacga cagcgaacgg acggacaggt acggacgcac ccttgcctac gtcttcgccg
    15181 gtggtgaggg ctctgaactg tttcttaacg agatactcgt ccgggaggga cttgcacaac
    15241 ctctcatcat tcctccgaac cggaggtacg cggggcgaat cctcaaggcg ttcgaggagg
    15301 cgcgtgccgg ccaaagaggc ctttggaaca gggcggaagg gagggtgttt acctcggcgc
    15361 aggtgtggag cgaagcgccg ttgcttgccg gttcgtttct cactctcttc atgacgattg
    15421 aaaaggccga actcaagggg cggcgcatcc tcgtcacccg gccgaagacg ggcaacaccc
    15481 gcatg
//
LOCUS       DAOPKU010000006        14745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_4277_length_14745_cov_4.948673, whole genome shotgun sequence.
ACCESSION   DAOPKU010000006 DAOPKU010000000
VERSION     DAOPKU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 14745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14745
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4277_length_14745_cov_4.948673"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PK849_00410"
     CDS             complement(<1..650)
                     /locus_tag="PK849_00410"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPE64611.1"
                     /translation="MIEKVKAASAFLKERISSSPESAVILGSGLGSFADSLEGRTVIP
                     YEEIPHWPLSTAPGHGGRLVHGRLGDRELLVMQGRVHYYEGYPMEEVVFPVRVLAQLG
                     VKNLVITNASGGVDVSLSPGDIVLLEDHVNFMGANPLRGPDTPGWNDRFPDMTRCYDE
                     KFIAAFEAAGAAEGAPVRRGVYFAFSGPSFETPAEIRMARALGATVVGMSTVPEAMT"
     gene            complement(683..1576)
                     /locus_tag="PK849_00415"
     CDS             complement(683..1576)
                     /locus_tag="PK849_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPE64612.1"
                     /translation="MTLYDFESAHESFLAWLLLERGLSRNTASAYSRDLHKWFAYCTT
                     NGILPFPPDEDGIARFQRFLVESEGSKASRQRTMAGLRTWMRFLQVEEVLEADVALPP
                     LPQREQRLPKILSEGEVDRLLNVCGGDDPLSQRDRALFEVAYGCGLRASEICSLRVGD
                     VDFAAKTLRTLGKSEKERAVPFLGEPAHRVRRYLEEARPRLAKEETGILFLSRNGMML
                     NREDIWRIIQKRGAEAGISKQRLHPHILRHSFATHLLRRGMDQKTLQELLGHSSIATT
                     DKYLHFDLELRDVYDASHPRA"
     gene            complement(1679..2224)
                     /locus_tag="PK849_00420"
     CDS             complement(1679..2224)
                     /locus_tag="PK849_00420"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPE64613.1"
                     /translation="MSEGVREKTLAQKTVYSGRILNLRVDDVELPGGKRAIREVVEHK
                     PAVGILPVMEDGTVLLIRQYRYAVGEDMLEIPAGIVEGNEPFDDAARRELQEEVGYFP
                     EHLREIGRIYNSPGFSTELMALFVATGLRPSKLQEDDDEFITVVPVAKEEIPALITGG
                     KLKDAKTFAALSWLMAAGEVH"
     gene            complement(2209..2766)
                     /locus_tag="PK849_00425"
     CDS             complement(2209..2766)
                     /locus_tag="PK849_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE64614.1"
                     /translation="MSFFKTLLAAGFGGQGVMVLGQLVAYTGIEEGRHVTWIPSYGPE
                     MRGGTANCGVVLSDDEIGSPVVDKADVLVVMNQPSLSKFIDRLKPGGVLVYNSNLVEY
                     DNSRKDIRVVALPATTLAEELGSDKITNVVILGAVVESSGIVADAVCVETIKEKLGKK
                     KPQFLPMNLGAYEKGKEVARECLKA"
     gene            complement(2779..3525)
                     /locus_tag="PK849_00430"
     CDS             complement(2779..3525)
                     /locus_tag="PK849_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE64615.1"
                     /translation="MSEVKVYERPKSWMPNVSSHYCPGCGHGIAHRLVCEAIDELGIQ
                     NDTIGVAPVGCAAMIYDYMDTDYCEAPHGRAPAVATGLKTVRPDKIVYTYQGDGDLAS
                     IGMGEIIHAANRGQNITVIFINNAIYGMTGGQMAPTTLIGQRASTCPLGRDVKLNGYP
                     IRVSELLAALPAPGYVTRVTVAQPKYIIKAKQAVKQAFQNQLDGKGFSLVEILSSCPT
                     NWGMRPVEAFDWLVNTMMPYYPLGTFKDFD"
     gene            complement(3541..4602)
                     /gene="vorB"
                     /locus_tag="PK849_00435"
     CDS             complement(3541..4602)
                     /gene="vorB"
                     /locus_tag="PK849_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPE64616.1"
                     /translation="MGRVLMKGTEAIAEAAIQAGCKYFFGYPITPQNEIPEYMSAHLP
                     EVGGVYVQAESEVASINMVLGGGATGYRVMTSSSSPGISLMSEGISYVAGCEIPAVIV
                     NVMRAGPGLGGILPGQADYLQSTKGGGNGDYNLIVLAPSTLQECVDLTQLAFDLAQKY
                     RNPVMVAADGFMGQMMEAVEIKPSPSKDQPADPSWALGFMGERGGKRSHLHSLFLSPE
                     PLEEHNRKLQKKYQAMKDAEQRCECYMMDDAEVLIAAYGTTARISRSAVNNLRAEGLK
                     VGMIRPITLWPYPYEAFKKLPSTVKHILVSEMNAGQMVDDVKVATGCKYPVSFYGRMG
                     GFAPSVGEIENECRRILGL"
     gene            complement(4629..4847)
                     /locus_tag="PK849_00440"
     CDS             complement(4629..4847)
                     /locus_tag="PK849_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPE64617.1"
                     /translation="MAKKGRIEVFEQFCKSCGLCVAACPVKVLRISEKTNAKGYRPVE
                     QFKEGCIGCGMCAMTCPDAAIEVFKIEE"
     gene            complement(4886..5674)
                     /locus_tag="PK849_00445"
     CDS             complement(4886..5674)
                     /locus_tag="PK849_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64618.1"
                     /translation="MNASEREQISQLMASFQTFASTLEKWPKAVAVTGALGSGKTEWV
                     LNLALGFEAIGDQVTIADLDIINPYFCIRQVTDALGARGFNIIMPPENARWSDMTVVS
                     PKISSALATDEGRLLLDIGGDAEGALALKQFEPDIRRAGYLLILVINAFRPQTSSVRG
                     VRTMLERMESICGLSVGALISNSHLMDETTAEHCADGLETVLEAGSELGLPVLYAGVP
                     PKLFPEAASVWKSRNLSVPCWPLSRYMMLPWEEGAMWSRPSTGE"
     gene            complement(5671..6618)
                     /gene="fmt"
                     /locus_tag="PK849_00450"
     CDS             complement(5671..6618)
                     /gene="fmt"
                     /locus_tag="PK849_00450"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPE64619.1"
                     /translation="MKDAFQRNPVLWFFGTGTFAASCLKHLVKNVHFDCIVTSPPSSG
                     GRGMRNILSPVETLCETLHLHVIRSSKVSEEPELLHHLAESPPDCILVVDFGQKILEP
                     FLSAPAWGCLNIHPSLLPSYRGAAPVPRALLNGDAVTGVSLFRLVEEMDAGPILFRKE
                     LTVGETASAGEILEILAEKGSELYLLGVKCLIEGSCKFRDQNSESATYAPKIANKEAE
                     CSWVWPSHRLVSTVRAMNPSPGAYIFIGAKRMKIWEVLHHPSLGRPGEIVDFLDGFPL
                     VGTSDGSVALLCVQPEGKKRLSGAEWARGIRLKKGDFLQ"
     gene            complement(6615..7133)
                     /gene="def"
                     /locus_tag="PK849_00455"
     CDS             complement(6615..7133)
                     /gene="def"
                     /locus_tag="PK849_00455"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPE64620.1"
                     /translation="MNSLRLRVYPDPVLRKETEPVVAFDDDLKDLVAEMIRLMNLHDG
                     VGLAAPQAGVSSRLAVVFHDGMLHVLANPVLLSSEGEQDGEEGCLSFPGIFGNVHRPS
                     RITIRHNDLDGVEHETSVEGFLARAFLHEMDHLNGRLLIDNFSPMRRAMARKKLLRSK
                     EGEDAGGGSSRR"
     gene            complement(7201..8763)
                     /locus_tag="PK849_00460"
     CDS             complement(7201..8763)
                     /locus_tag="PK849_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPE64621.1"
                     /translation="MTEEMMNEGLRTTDVENAEGREVEETGEAQETMESILEQYGEVE
                     ELHRGKIVTGTVVNVVDGGWLVDVGYKCEGFLPAKEWSHRVLVEDEAEPAIEEKIQIQ
                     VVSIRHGEEAQLLVSRWRCEFDRRWSELESKLAQNEVIPVKGIRKVKGGLMVECCGLE
                     GFVPISHLAEEGRGVNPGKFVGEVFDAKLMEKDKKKHRLVFSRRMIVEETLVAERESF
                     YTDVAEGSVLDGEVSSITSFGVFVNVGPIDGLVHMSELSWKRNIKPKEMFKKGDPVKV
                     RVIGIDRENNRVSLSIKQTENDPWTTASSKWKKNEVTKGVVTNVTDFGAFVEVEPGIE
                     GLIHIGDLSWTRIKHPRDVLRKGQEVEVQVLDVDMEKKRMSLGFKQLNDPWNDIATRY
                     SKDQDLTVKVVRLADFGAFVEIEEGVEGLIHISQLSTKRVEKPGDVLSEGQEVLARII
                     EINPSDRRMRLSISALEEPAGESSARPRDEERRRGKQERGDHDRHPQKNVMSDGEANV
                     TIGEFLKNSLQD"
     gene            complement(8741..9661)
                     /gene="ispH"
                     /locus_tag="PK849_00465"
     CDS             complement(8741..9661)
                     /gene="ispH"
                     /locus_tag="PK849_00465"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPE64622.1"
                     /translation="MEHKRVIETASPTGFCFGVQRAIESLEKRIEECASSASGSDGRV
                     YSIGMPIHNPQEVERLSRKGLRVVDRIEDIPCGSAVFVRAHGVPPSVKEELISRCGAE
                     NVTDATCPFVKNAQDKAAFLAGEGYSVLVLGDPGHPEVQAIVGCAGGDVLVASSLSEV
                     KALGKRDRLGVISQTTQKGEFLGSAASILAPQIRELRVFNTICGATTQRQEAVREITS
                     RVDGLLVVGGRNSANTAKLVEIARSRNCDVLWIEHAGELSGEWFSGKHKIGIAAGAST
                     PDWLIEEVKHAIETSQVSRGMEGYDGRNDE"
     gene            complement(9639..10655)
                     /gene="miaA"
                     /locus_tag="PK849_00470"
     CDS             complement(9639..10655)
                     /gene="miaA"
                     /locus_tag="PK849_00470"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPE64623.1"
                     /translation="MFSESGGTGTRTGDRLPIIAIVGPTAVGKTAVSLLLAERLGAEI
                     ISVDSRQVYRYLDIGADKISPDIRRRTIHHMIDVADPDQVFTAADFVSGCVDAVERIR
                     ARGRIPLFAGGTPFYFEALFGGMLSEELPKDEDLRRELEQIAAEQGAGALHATLRSVD
                     PESAGRLHENDVRRVVRALEICRLTGKTVAEAWSERKKMTPPKEYDVLYVGLTRERRF
                     LFESIERRVGEQFASGFVEEVEWLLNNGYDERFPSMQGFGYKDILEYLRGRCSREEAA
                     ERDIRQTKAFSRRQMTWFGKFSPILWYDTVDCSMTKLVDTIENDVRHYPGRWSFVNGA
                     QEGN"
     gene            complement(10661..12469)
                     /gene="mutL"
                     /locus_tag="PK849_00475"
     CDS             complement(10661..12469)
                     /gene="mutL"
                     /locus_tag="PK849_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPE64624.1"
                     /translation="MYSRIAAGEVVERPVSVVKETVENALDAGATEISVALHEGGKLR
                     IVIEDNGEGMSFDDLPLAVARHATSKIRTVEELETVRSLGFRGEALASIAVVSRLEIR
                     SRKAGEEQGALLSIEGGRTVRHVPTPCRPGTRISIEDLFYTLPARRKFLKTAAAEARR
                     VSALVRDLAVAYPAVAFLEQHDGKQVFSSPGDGDRESLLRAMWGDPRQAPRTSSGRAL
                     DAGFSGAELRMCEAAATNLSLECWWMPFPGKTRSSISTFVNGRTVTDSVIRGAVGALC
                     RSLVGNWIFFFALSPELLDANIHPAKAEVRFRYPGEVFDVVQQAVLTLSGKPQTIAPF
                     SAGVSSDGFDARRNAPLLGHQGNASGPSRSWQRQDASDGELFNRVAEAPFSRETATAP
                     PQSEESMGIFPTEQEAQRGEERDSVRLLGRMESGYVVFEDGDALAVMDPHAAHERIGY
                     ERARRLSKDSVTRQRCAVPLPLPPSFSMSVREHMEALEDIGFSFDEREGLLLLTVYPA
                     LQGGIGEDPVRLLRSVLLEWTEDRKSPLEDILWRKLATLACGLSVKLGDRLTASECLA
                     LWRGLMQCEQPWSCPHGRPTVLLLPRNKLESFFGRE"
     gene            complement(12474..>14745)
                     /gene="mutS"
                     /locus_tag="PK849_00480"
     CDS             complement(12474..>14745)
                     /gene="mutS"
                     /locus_tag="PK849_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPE64625.1"
                     /translation="AGSGGWLAAVCRAGKELAVALLSVETGRLEAGVFPKGEAAALLG
                     SFAPGELLHPASLSLSEWFPLAGDYFPLPREDEFFSPVQGTRRLLQQWKTATLASFGL
                     EDGSPAAGAAAAVLAYLHETQFGAVEYIKRAHPLRSSEHLHLDVTTGRNLELFDTRQE
                     QNGDAPSLYRTLNRCRSTMGRRLLREWLLRPLLDLGELRRRQDAVEWLAENGRERRAL
                     RDLLGGCRDVERSSSRLSLGTGTPKDLGAIRDTLYLLPSLLPLCADTPLSCWTGGIAD
                     FSALREELCAALEDDLPRNRTQGGLIRGGYDAELDEWRGVEAGASGMMDAYVEKVREI
                     TGISRIKAGYNKVFGYYLEVSRAALNAGEVELPGWFIRKQTLVNAERFITQEMKELEE
                     RIASAASEISRREGALFDGLTERTLARVEELQHLGSVLSALDVLTSFGEVARERGYIR
                     PELNDGYDIVVQNARHPVVEECLTDSPFVPNSIALRRSERRIALITGPNMAGKSTYLR
                     TAALLVVMAQIGSFVPAESAAMGLCDRIFTRIGARDDLARGSSTFMVEMVETANILNN
                     LTERSLVILDEVGRGTSTYDGMSIAWAVLEHLAEGCGCSPKVLFATHYHELTCLDERY
                     PGIENLSMAVKEDSDGIFFLHQVVKGSADRSYGIEVARLAGLPRPVLKRAFELLQRFE
                     KDEKCAAPPQHAAKRKEIPGIRQMTLFESARSGIVEEIAALTPDTLTPFRALELLYSL
                     VEKSREAIRSENLSSS"
BASE COUNT         3012 a         4743 c         3966 g         3024 t
ORIGIN      
        1 gtcatagctt ccggtacggt ggacattccc accacggtcg cccccaacgc gcgcgccatc
       61 cgtatttcgg ccggagtctc gaacgacggt cctgaaaaag cgaaatagac tcccctgcgc
      121 accggcgcac cctcagccgc tcctgccgct tcgaaagcgg ctatgaattt ttcgtcgtag
      181 catcgcgtca tatccgggaa acggtcgttc caccccggag tatcgggacc gcgcagcgga
      241 ttcgctccca tgaaattgac atgatcctcc agaagaacaa tatcccccgg cgagagcgag
      301 acgtcgactc cgcccgaagc gttggtgatc accaggttct tcacgcccaa ctgagcgagc
      361 acgcgcaccg ggaacactac ctcttccatc gggtatcctt cgtaatagtg aacgcggccc
      421 tgcattacga ggagctcacg atctcccagc cttccgtgga caagtcttcc tccgtgtccg
      481 ggagcggttg aaagaggcca gtgcggtatc tcttcatagg gtatcacggt acgcccttca
      541 agggaatccg cgaaagagcc caaccctgaa cccaaaatca ccgcagattc cggagacgaa
      601 gaaatacgtt ctttaaggaa agcgcttgcg gccttgactt tttcgatcat tctctcaact
      661 cctcttcctt aattttttcg ttttaagcac gtggatgaga agcatcatac acgtcccgaa
      721 gttcaaggtc aaagtgcagg tacttatcgg tcgttgcgat ggaggaatgc ccaagaagtt
      781 cctggagcgt cttctggtcc ataccgcgcc ggagaagatg cgttgcgaag ctgtgacgga
      841 ggatatgcgg gtgaagacgt tgttttgaaa tacccgcttc agctcccctc ttctgtatga
      901 ttcgccaaat atcctccctg ttcagcatca taccgttccg cgaaaggaag agaataccgg
      961 tctcctcctt cgccagacgc ggccgggcct cttcaagata cctccgcacc ctgtgggccg
     1021 gttctccaag aaacggcaca gcacgctcct tctcactctt cccaagcgtt ctgagcgtct
     1081 tcgcggcaaa atccacatcc ccgacccgga gagagcagat ctcgcttgcc cgcagtccgc
     1141 atccatacgc cacttcaaaa agcgcacggt ctctttgcga aagaggatca tcgcctccac
     1201 agacgttgag cagtctgtcc acctcccctt cgctgagaat tttcggaaga cgctgttctc
     1261 tctgcggcaa cgggggaagt gcgacatccg cctcaaggac ctcctcgacc tgaagaaagc
     1321 gcatccacgt acgcaagccc gccattgtcc gctgacgcga agctttgctc ccctcgctct
     1381 cgacaagaaa acgctgaaag cgagcaattc cgtcctcgtc gggagggaat ggaagaattc
     1441 cgttcgtcgt gcaatacgca aaccacttgt gaagatcacg agagtacgcg gaggctgtat
     1501 tccggctcaa cccccgctcc agcaacaacc acgcaaggaa agattcatgg gcggattcaa
     1561 agtcatataa tgtcataata gttacattgt agcataaaaa aaggaaccgg aaaaaccggt
     1621 tcctcaggcg tctgtaatag agtgcaacaa aatagttggc ggatcattca caagaaactc
     1681 agtgaacctc accggcggcc atcaaccacg agagcgcggc aaacgtcttg gcatccttca
     1741 gtttgccccc cgtaatcaag gccgggatct cctccttggc aacaggaaca accgtgataa
     1801 actcgtcgtc gtcctcctgg agcttcgacg gacgcagccc ggtagcgaca aacagcgcca
     1861 tcagctccgt actgaacccc ggagagttgt agatacgtcc gatctcccgc aaatgctccg
     1921 gaaagtagcc cacttcttcc tggagctctc ttcgagcggc atcgtcgaaa ggctcgtttc
     1981 cctcgacaat tcctgcggga atttcgagca tatcctcccc gacagcgtaa cggtactgcc
     2041 gtatcaaaag aacggtcccg tcttccatga ctggaaggat accaacagca ggcttgtgct
     2101 ccacgacctc gcggatcgcc cgcttccccc cgggaagctc aacatcgtcc acacggaggt
     2161 tcagtattct tccggaatag acggtcttct gcgcgagggt cttctccctc acgccttcag
     2221 acattccctg gcaacctctt ttcccttttc gtacgcgccg aggttcatcg ggaggaactg
     2281 cggcttcttt ttacccagct tctccttgat cgtctccacg cacacggcgt cggcgacaat
     2341 accgctcgac tccacgaccg cgcccagaat aacgacgttc gtgattttgt cgctgcccag
     2401 ctcctcggcg agggtggtcg cgggaagggc gacgacgcga atatccttac gtgagttgtc
     2461 gtattcgacg agattgctgt tgtacaccag gacgccgccc ggcttcagcc tgtcgatgaa
     2521 cttggaaagc gacggctggt tcatcacaac aaggacgtcc gccttgtcga caacgggcga
     2581 accgatctcg tcgtccgaaa gcaccacgcc gcaattcgcc gttccgccgc gcatctccgg
     2641 accgtacgag ggaatccacg ttacatgtct gccctcttcg atccccgtat acgccacgag
     2701 ctgtcccaaa accatgactc cctgaccgcc gaatccggcc gcgagaagcg tcttgaaaaa
     2761 tgacatgctc ttccctccct agtcgaaatc cttgaacgta ccaaggggat agtagggcat
     2821 catcgtgttg acaagccagt cgaaggcttc gacaggacgc attccccagt tcgtggggca
     2881 ggaggagagt atctcgacga gggagaaccc cttcccgtcc aactggttct ggaaggcctg
     2941 tttcacagcc tgcttcgcct tgatgatgta ctttggctgc gccaccgtca cgcgggtcac
     3001 gtagccgggc gcaggaagcg ccgccagaag ttcgctcacg cggatcgggt agccgttgag
     3061 cttgacgtcg cgtccaagcg gacatgtgga ggctctctgc ccgataagcg tcgtcggcgc
     3121 catctgaccg ccggtcatac cgtaaatcgc gttgttgatg aagatcaccg tgatgttctg
     3181 ccctctgttt gcggcatgaa tgatctcgcc catgcctatc gaggcaaggt cgccgtcgcc
     3241 ctggtaggtg tacacgatct tgtcgggacg gacggtcttt aatccggtcg ccactgcagg
     3301 agcgcgcccg tggggcgctt cgcagtagtc ggtgtccatg tagtcgtaga tcattgctgc
     3361 gcaccctacc ggcgcgacgc ctatggtatc gttctgtatg ccgagttcgt cgatagcctc
     3421 gcagaccaga cggtgggcga ttccgtgccc gcaaccgggg cagtagtggc tgctgacatt
     3481 cggcatccaa ctcttcggac gttcgtagac ttttacttca ctcatcagaa atcccctccc
     3541 ttacaggccg agaatccgtc ggcattcgtt ctctatctcc ccgaccgagg gggcgaatcc
     3601 gcccatgcgg ccgtagaagg agacagggta cttgcaacct gtggcaactt tcacgtcatc
     3661 caccatctgt ccggcgttca tctccgaaac aagaatgtgc ttgacggtgg aaggaagctt
     3721 cttgaaagcc tcgtaaggat acggccacag tgtgatcggg cggatcatgc cgaccttgag
     3781 cccttcagcg cgcagattgt tcacggcgga gcgggagatg cgggctgttg taccgtaggc
     3841 ggcgatgagc acctcggcgt cgtccatcat gtagcactcg cagcgctgct cggcatcctt
     3901 cattgcctgg tacttcttct gcagttttct gttgtgctcc tccaaaggtt ccggagagag
     3961 gaagaggcta tgcaggtggc tgcgctttcc gcccctttct cccatgaaac cgagagccca
     4021 tgacgggtcg gccggctggt ctttcgacgg actcggctta atctcaacag cttccatcat
     4081 ctgccccatg aacccgtcgg cggcaaccat gacaggattg cggtacttct gggcaagatc
     4141 gaaagcgagc tgggtaagat cgacgcactc ctgcaacgtg ctgggagcga ggacaatcag
     4201 gttatagtct ccgttgccgc cccccttggt cgactgaaga tagtcggcct gtccaggaag
     4261 aatgccgccc aatcccggac cggcgcgcat cacgttcacg atgaccgcgg gaatttcaca
     4321 acctgcaacg taggaaatcc cttccgacat aagcgaaata ccggggctcg aggaggatgt
     4381 catcacccga taccctgtgg cgcctcctcc aaggaccatg ttgatcgagg ccacttcgct
     4441 ctccgcctgc acatacactc ccccgacctc gggaagatgc gccgacatat actccggaat
     4501 ctcgttctgc ggcgtaatcg gatacccgaa gaagtattta cagccggcct gaatggccgc
     4561 ctcggcaata gcttcagtac ctttcatcaa aactcgtccc atgtctactt cacctccggc
     4621 aagacaaact actcttcaat tttgaaaact tcgatcgccg catcgggaca ggtcatcgca
     4681 cacatgccgc acccgatgca cccctccttg aactgttcga ccgggcgata ccccttggcg
     4741 ttggtctttt cggaaatacg aaggaccttc accggacagg cagccacaca cagtccgcag
     4801 cttttgcaaa actgctcgaa aacctcgatg cgcccctttt tcgccattgc ctgtgcaccc
     4861 cttccacaaa aaagattgga gaaaattatt cgcccgtcga cggcctggac cacatggccc
     4921 cttcctccca cggaagcatc atgtaccggg agagcggcca acagggaacg gaaagattcc
     4981 gggatttcca cacggaagcg gcctcgggaa acagtttcgg aggaacgccc gcgtacagca
     5041 cgggaagacc gagttcgctt ccggcttcca gaactgtttc aagtccgtcg gcacaatgtt
     5101 ccgccgtcgt ctcgtccatg agatgagagt tgctgataag cgcccccact gaaaggccgc
     5161 agatactctc cattctttcc agcattgtcc gcacaccgcg tactgaagac gtctgcggac
     5221 ggaaagcgtt gataacgagg attaaaagat accccgcgcg gcggatgtcc ggctcgaact
     5281 gcttcagcgc cagcgcgccc tccgcgtccc ctccgatatc cagaagcaga cgcccctcgt
     5341 cggttgcgag cgcgcttgag attttcggcg acacgacggt catgtcggac catctggcgt
     5401 tttcaggggg catgatgata ttgaaccctc ttgcgcccag cgcatccgtt acctggcgga
     5461 tacaaaaata ggggttgata atatccaggt cggcaatcgt aacctgatcg ccgatcgctt
     5521 caaaacctaa agcgagattg aggacccatt cggtctttcc ggagccgagc gcgcctgtta
     5581 cggcaaccgc cttcggccat ttttcaagag tggaggcaaa tgtttgaaag gacgccatca
     5641 actggctgat ttgttcgcgt tcagaggcgt tcattgaaga aaatcccctt tcttcaggcg
     5701 gattccgcgg gcccattccg cgccgctgag gcgttttttc ccctcaggct gaacacaaag
     5761 aagcgcgaca gacccgtctg acgtaccaac aagaggaaat ccgtcgagaa agtctacgat
     5821 ttcgccgggg cgccccaggg atggatgatg gaggacttcc caaattttca tccgttttgc
     5881 gccaataaaa atataagcgc ccggagaggg gttcatcgcc cgaacggtgg agacaagacg
     5941 atgcgagggc cagacccaag aacattcagc ctctttgttg gcaattttag gtgcatatgt
     6001 tgctgattct gaattttgat ctctaaattt gcaacttccc tcaataagac attttacacc
     6061 caacagataa agttcgctac ctttttctgc aagaatctcc aaaatttctc cagcgcttgc
     6121 ggtttcgcct accgtcaact cttttcgaaa gaggattggc cccgcatcca tctcctctac
     6181 gagacggaag agagaaaccc cggttacagc gtctccgttc agaagcgccc tcggcacagg
     6241 ggcagcccct ctgtatgaag gaagcaacga cgggtggatg ttcaaacacc cccacgcagg
     6301 cgccgagaga aagggctcca gtatcttctg cccgaaatcc acaacaagaa tacagtccgg
     6361 aggcgactct gcgaggtgat gaagcagttc cggctcttct gaaaccttgg aggaacggat
     6421 cacatggagg tgaagtgttt cgcagagtgt ctcaacagga gagaggatgt tgcgcatacc
     6481 tcttccccct gaagagggcg gcgaggtcac gatacaatcg aaatgaacgt tctttacgag
     6541 atgcttcagg cagctcgccg caaacgtccc ggttccgaaa aaccagagga cggggtttct
     6601 ttggaaggcg tccttcatcg tctgctcgaa cctccgccgg cgtcttcccc ttcttttgaa
     6661 cgcagtaact tttttcgcgc catggcgcgc ctcattggag agaagttatc gatgagcagc
     6721 ctcccgttca gatggtccat ttcgtgtaga aacgcgcggg caagaaagcc ctccacagac
     6781 gtctcgtgtt caaccccgtc gaggtcgttg tgccgtatgg tgatcctgga aggccggtgg
     6841 acattgccga aaatccccgg aaaactcagg catccttctt ccccgtcctg ttctccttcc
     6901 gaagagagca gcaccggatt ggccagaaca tggagcattc cgtcgtgaaa cacaacggcg
     6961 agccgggaag aaactccggc ttgcggagcc gccaatccta ccccgtcgtg cagattcata
     7021 agccggatca tctcggcgac aaggtctttc aggtcatcgt caaaagcaac gaccggctct
     7081 gtctctttcc tcagaaccgg gtcgggatac acacgaagcc gcaatgaatt cattatattc
     7141 gccgcacttt ctttgtaaat aaaaagggcc gcctttttta aggcgaccct ttgacgtacg
     7201 ttagtcctgc aaggagttct tcaaaaactc tccgatggtg acgttcgcct cgccgtcgct
     7261 catcacgttc ttctgcgggt gacggtcgtg atcgccgcgt tcctgcttcc ctctgcggcg
     7321 ttcctcgtcg cgggggcggg cggaagactc tccggccggc tcttcaagtg cgctgatgct
     7381 caggcgcatt ctgcggtccg aaggattgat ctcgatgata cgggcgagaa cttcctgccc
     7441 ctcggagagc acgtcgcccg gtttctcaac cctctttgtg ctcagctggg agatgtggat
     7501 cagcccctcg acgccctctt cgatctcgac gaaagcgccg aaatcagcaa gccggacaac
     7561 cttgacggtc agatcctgat cctttgaata cctggtggca atatcgttcc acgggtcgtt
     7621 cagctgcttg aagccgagac tcatgcgctt cttctccata tcgacgtcga gcacctgcac
     7681 ctcgacctcc tgccccttgc gaagcacatc tctcggatgc tttatgcggg tccagctaag
     7741 atcgccgatg tggatgaggc cttcgattcc gggctcgacc tcgacgaatg cgccgaagtc
     7801 ggtcacgttt gtgacaactc ccttggtgac ctcattcttc ttccacttgg aagaagccgt
     7861 ggtccacgga tcgttctcag tctgcttgat gctgagagaa acgcggttgt tctcgcggtc
     7921 gatcccgatg acgcgcacct tgaccgggtc gcccttcttg aacatctcct tcggcttgat
     7981 gttccgcttc caggaaagct cgctcatgtg gacgagtccg tcgatgggac cgacgttcac
     8041 gaagacgccg aaagatgtga tgctgcttac ttctccgtca agaaccgagc cttccgccac
     8101 gtcggtgtag aaagactcgc gctctgcaac cagcgtctcc tcgacgatca tacgtctgga
     8161 gaagacgagc cggtgcttct tcttgtcctt ttccatcaat ttagcgtcga agacctcgcc
     8221 cacgaatttg ccgggattga cgcctctgcc ctcttcggca agatgcgaaa tggggacgaa
     8281 cccctcaagt ccgcagcact ccaccatgag accgcccttg accttgcgga tccccttcac
     8341 tggaatgact tcgttctggg caagtttgct ttcaagttcg ctccagcggc ggtcgaactc
     8401 gcatctccaa cggctgacaa gaagctgcgc ctcttcgccg tgacgaatgc tcaccacctg
     8461 gatctggatt ttttcttcga ttgcgggctc cgcttcatcc tcgaccagaa ccctgtgcga
     8521 ccattccttt gcgggaagga agccctcgca cttgtatcct acgtccacaa gccagccgcc
     8581 gtcgacgacg ttcaccactg taccggtgac aatctttccc ctatgaagct cctcgacctc
     8641 tccatactgt tcgaggatgc tctccatcgt ctcctgtgct tcgcccgttt cctcaacttc
     8701 gcgcccttcg gcgttttcga cgtccgttgt acggagtcct tcattcatca tttcttccgt
     8761 cataaccttc catccccctt gacacctgcg atgtctctat tgcgtgtttc acttcttcta
     8821 tcaaccagtc cggtgtgctg gcgccggctg ctattccaat tttgtgtttc cctgagaacc
     8881 actcgccgga aagctcccct gcatgttcaa tccacaggac gtcgcagttg cgcgaccggg
     8941 ctatttcgac gagcttcgcc gtgttggcgc tgtttcggcc tccgaccacg agcagtccgt
     9001 cgacccgcga ggtaatctcc cgcacggcct cctgacgctg cgtcgtggcg ccgcatatcg
     9061 tgttgaacac ccgcaactcg cggatttgcg gcgcaagtat ggaggcggcg gatccgagga
     9121 actcgccctt ctgggtcgtc tgagatatta ctcccaacct gtcccttttg ccaagagcct
     9181 ttacctcgga aagagaggat gcgaccagaa cgtcgccccc cgcgcacccc acgatggcct
     9241 gcacttcagg gtgtccggga tcgccgagca caagcaccga gtacccctcg cccgcgagaa
     9301 aagcggcctt gtcctgcgcg ttcttcacga acggacaggt cgcgtcggtg acattctcgg
     9361 cgccgcaccg cgaaatgagc tcctccttca cagagggagg aacgccgtgc gcacggacga
     9421 aaacagcgga gccgcatgga atatcctcta tccggtcgac aactcgaagc cctttccggg
     9481 aaagacgctc cacttcctga ggattgtgga tcggcattcc gatggaatag actcttccat
     9541 cgcttccgga agccgaggaa gcgcactctt caatgcgttt ctccagactt tctatggccc
     9601 gttgaacacc gaagcaaaaa ccggtggggg acgcagtctc aattaccctc ttgtgctcca
     9661 ttgacgaaag accatctccc aggatagtgc cgtacatcgt tttcgatcgt atcgacgagt
     9721 ttcgtcatcg aacaatctac agtatcatac catagaattg gagaaaattt cccaaaccag
     9781 gtcatctgcc ttcgagaaaa tgcttttgtt tgccgtatat cccgttcggc tgcctcttcg
     9841 cgcgagcagc gcccccgcag atactccaga atatccttat atccgaaccc ctgcatggac
     9901 ggaaaacgct cgtcgtatcc gttgttgaga agccactcca cctcttctac aaacccagag
     9961 gcgaactgct ctccgacccg ccgttcgatg ctttcgaaga gaaagcggcg ttcgcgcgtc
    10021 aggccgacgt acagaacgtc atactctttc ggcggcgtca tcttttttct ttcgctccac
    10081 gcctccgcga cggtctttcc ggtcaaacga cagatctcca acgcgcggac gacccgccgg
    10141 acatcgttct catggagcct tccggcgctc tcagggtcga ccgaacgaag cgtcgcatgc
    10201 agcgcgcccg ctccctgttc cgcggcgatt tgctccagct ctctccgcag atcttcgtcc
    10261 ttgggcagtt cctcggagag cattccgccg aagagcgctt cgaagtagaa gggcgtgcct
    10321 cccgcaaaga gaggaatccg cccgcgcgcg cgtattcttt ccaccgcgtc cacgcagcct
    10381 gagacgaagt ccgccgcggt gaacacctgg tccgggtccg ccacgtcgat catgtgatgg
    10441 atagttcgcc gtcgaatatc cggcgaaatc ttgtccgcgc cgatatccag gtacctgtaa
    10501 acctggcgtg aatccaccga gatgatctcc gcgccgaggc gttccgcaag cagaaggctg
    10561 acggcggttt ttcccaccgc ggtcggccct acaatggcga tgatcggcag acggtcgccc
    10621 gtcctcgttc cggtaccgcc ggattctgaa aacatgcagt ctactccctt ccgaagaacg
    10681 attccagttt atttcgcggc agaagcagca cggtcggccg tccatgaggg cacgaccacg
    10741 gctgttcgca ctgcatcagg cccctccata aggcgaggca ctcggacgcg gtcagccggt
    10801 cccccagctt gacggagagt ccgcacgcaa gcgtcgccag ctttctccag aggatatcct
    10861 ccagcggact cttccggtcc tccgtccact ccagaagcac cgagcgcaga agacggacag
    10921 ggtcctcccc tatacctccc tgcagcgccg ggtagaccgt aaggaggaga agcccctcgc
    10981 gctcgtcgaa ggagaagccg atatcctcaa gagcctccat gtgctcgcgg acggacatcg
    11041 agaaggacgg cggaaggggg agcggaaccg cgcagcgctg ccttgtcacc gaatccttcg
    11101 agagcctgcg cgcccgctca tatccgatcc gctcgtgcgc cgcgtgcggg tccatcaccg
    11161 caagagcgtc cccgtcttcg aagacgacgt accccgactc catacgcccc aacagccgca
    11221 cggagtcgcg ctcctcgccc ctctgcgctt cctgttcggt cgggaagatt cccatgcttt
    11281 cttccgattg aggaggagcg gttgcggtct ctcgggaaaa cggcgcttcc gcgaccctgt
    11341 tgaagagttc gccgtccgaa gcatcctgac gctgccatga gcggctcggc cccgaggcgt
    11401 tcccctgatg cccaaggagc ggagcgttcc gacgcgcatc gaatccgtcc gaagacactc
    11461 ccgcagagaa cggagcgatg gtctgcggtt ttccggagag cgtgagcacg gcctgctgca
    11521 ccacgtcgaa gacctctccc ggataccgga agcgcacctc ggcctttgca gggtggatat
    11581 tcgcgtccag cagctcgggc gaaagagcga aaaagaaaat ccagttgccc accagcgatc
    11641 tgcacagagc gcccaccgcg ccccgaatga cggaatccgt caccgtccgt ccgttcacga
    11701 atgtcgaaat ggagcttctc gtcttcccgg ggaagggcat ccaccagcac tccagcgaaa
    11761 gattcgtcgc cgccgcctcg cacatacgga gttccgcgcc cgagaacccg gcgtcgagcg
    11821 ctctgccgga ggaagtacgc ggcgcctgac ggggatcccc ccacatggcg cgaagcagcg
    11881 actctctgtc gccgtccccc ggagaggaga agacctgctt gccgtcgtgc tgttccaaaa
    11941 aagcgacggc ggggtacgcc acggcgagat cgcgcaccag cgccgagact cgccgcgcct
    12001 ccgccgccgc agttttcagg aattttctgc gcgccgggag cgtatagaaa agatcctcga
    12061 tggagatgcg cgtccccgga cggcagggcg tcggcacatg gcgaacagtg cgccctccct
    12121 cgatcgagag caacgcgccc tgctcctccc cggctttccg cgaacgaatc tccaggcggc
    12181 tcacgacggc gatgctcgcc agcgcctccc cccggaatcc gagcgagcgg acggtctcca
    12241 gctcctcgac ggtgcgtatt ttgctcgtcg cgtggcgggc tacggccaga ggcagatcgt
    12301 cgaacgacat tccctcgccg ttgtcctcga tgacgatacg gagctttccg ccctcgtgga
    12361 gagctacgga aatctccgtc gctcccgcgt caagcgcgtt ctccaccgtc tccttcacca
    12421 cggaaacggg gcgttcgacc acctctcccg ccgcgatgcg ggagtacacg tcctcaggaa
    12481 gaagagagat tttcagagcg aatcgcctcc ctgcttttct cgaccagcga atagagtaat
    12541 tccagcgcgc ggaagggtgt cagcgtatcc ggcgtcagcg ccgcgatctc ctcgacgatg
    12601 ccggagcgcg cggactcgaa cagcgtcatc tgccggattc cggggatctc cttccgtttg
    12661 gcggcgtgct gcggcggcgc ggcgcatttc tcatccttct cgaagcgctg caacagctcg
    12721 aacgccctct tgagcacagg gcgaggcaat cccgcaagac gcgcgacttc gataccgtac
    12781 gaccggtccg cgcttccttt caccacctga tgcagaaaga agatgccgtc cgaatcctcc
    12841 ttcacggcca tgctcaggtt ctcgatcccc ggatagcgct cgtcgaggca ggtcaactcg
    12901 tggtagtgcg tcgcgaagag caccttcggc gagcagccgc acccctccgc cagatgctca
    12961 aggacggccc aggcgatgct catgccgtcg taggtggacg tcccgcgccc cacctcgtcg
    13021 aggatgacca gactcctctc cgtcaggttg ttcaggatgt tcgccgtctc caccatctcc
    13081 accatgaagg tgctgctccc gcgcgccaag tcgtcccgcg cgccgatgcg cgtgaaaatc
    13141 cggtcgcaga gtcccatcgc cgccgactcc gcaggaacga agctcccgat ctgcgccatc
    13201 acgacgagga gcgcggcggt ccgcaggtag gtggactttc ccgccatgtt cggccctgtt
    13261 atcagcgcga ttctcctctc ggaacgccgc agcgcgatgg agttgggaac gaagggcgaa
    13321 tcggtcaggc actcctccac aaccgggtga cgggcgttct gcacgacgat atcgtacccg
    13381 tcgttgagct ccgggcggat atacccccgc tccctggcga cctcgccgaa ggaggtcagc
    13441 acgtcgagcg ccgacaggac gctccccaga tgctgcaact cctccacgcg agccagcgtc
    13501 cgctccgtca ggccgtcgaa gagcgcgccc tccctccgcg agatctccga cgccgcagac
    13561 gcgatacgct cctcaagctc cttcatctcc tgcgtgatga agcgctccgc gttcaccagc
    13621 gtctgcttgc ggatgaacca cccgggcagc tccacctcgc cggcgttcag agcggccctg
    13681 ctcacttcga gatagtagcc gaacaccttg ttgtacccgg ccttgatccg cgagatgcct
    13741 gtgatctcgc gcaccttctc cacgtaggcg tccatcatac cggaagcgcc cgcctcgacg
    13801 ccgcgccact cgtccagctc cgcgtcgtac ccgccgcgaa tcagaccgcc ctgcgtcctg
    13861 tttcggggga ggtcgtcctc aagggccgcg cacagctcct cccgaagcgc cgaaaaatcg
    13921 gctatccctc cggtccagca agaaagcggc gtgtcggcgc acaacggaag tagagaaggc
    13981 agcagataga gggtatcccg gatcgcgccc aagtctttcg gagtgccggt tccaaggctc
    14041 agccgcgaag aggagcgctc cacgtcgcgg catccgccga gcagatcgcg gagcgcgcgc
    14101 cgctctctgc cattctccgc aagccattcg acggcatcct gcctgcggcg cagctcgccg
    14161 aggtcgagca gcggacggag cagccactcc ctgagcagcc gtctccccat ggtcgaacgg
    14221 cagcggttca gcgtccggta gagggacgga gcgtctccgt tctgctcctg gcgcgtatcg
    14281 aaaagctcaa gattccgccc cgtcgtcacg tccagatgca gatgctccga cgagcgcaac
    14341 gggtgcgccc gctttatgta ttccaccgcg ccgaactgcg tctcgtggag atacgccagt
    14401 acggcggcgg cggctccggc agcgggagag ccgtcctcca ggccgaagga ggcaagcgtc
    14461 gccgtcttcc actgttgcag cagacgccgc gttccctgga ccgggctgaa gaactcgtcc
    14521 tcccttggga gagggaagta gtcgcccgca agcgggaacc actcggagag cgaaaggctc
    14581 gcgggatgca gcagctcccc cggagcgaag gaaccaagca gcgcggcggc ctcccccttc
    14641 gggaagacgc ccgcctcaag acggccggtc tccacggaga gcagcgccac ggcaagctcc
    14701 ttcccggcgc ggcatacggc cgccagccat ccgcccgatc cggcc
//
LOCUS       DAOPKU010000007        13966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_4582_length_13966_cov_4.911581, whole genome shotgun sequence.
ACCESSION   DAOPKU010000007 DAOPKU010000000
VERSION     DAOPKU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13966
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4582_length_13966_cov_4.911581"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PK849_00485"
     CDS             <1..646
                     /locus_tag="PK849_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HPE64626.1"
                     /translation="TVIDVNTGKYIGKTDLRHTVLETNLEAASEIARQLRLRAIGGIV
                     VIDFIDMDFEEDRSRLLRRLEEVFQPDRYRARVFGVSQLGLVEITRKRARPDIRSMLT
                     RSCPACGGGGWVFREDTIAMAIKRFLRKVSFANRAEALLLEANAAVAQYIFETYLMLW
                     EAELGRKIFIAAAPDFAWSKFRLEAQGGVDQVERRIEQMEKREARIIVYRTSSS"
     gene            621..4253
                     /gene="smc"
                     /locus_tag="PK849_00490"
     CDS             621..4253
                     /gene="smc"
                     /locus_tag="PK849_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPE64627.1"
                     /translation="MYIERLHLKGFKSFGASLDLLLSPGFTAIVGPNGSGKSNLLDAL
                     RWVLGEAGSQRLRIVKQGDLLFSGSVTLPPASRAEVSLLLRSTPYSGRESAHSPEAGQ
                     CVLRRSYSSETGTVLTVDGTRVRLSDLDEVKRLWHLEGDQFAFIGQGEVAEAIHQRPA
                     QRRAHLEVLFGIDQYRKKRNETSQKLVAASEEVLRLGALAAELTHRREEIAPAVEIAQ
                     AAKKITDELEEKRRGYYFFRRRLLEEKSAALGEDLRTLREEEALRLRWKELWEAMYTR
                     SVQQCGEQEERVKRLLGDRDELVARREGLRRSCFAGAASIREILSRRRALAEEQKETA
                     ARLASLTKESEELKSKEASLHVEHGSRASERELLRKKVRELHEAIDAERRRKDETTNT
                     LASLSGELETLRSRLRSRHSFLETCGADLAASEEALRTLEESHAAASRNLEQLEEREP
                     ELVEAHSEIFARCRTTGGTLQSVRKEASALEAALEDLRSAEDSMYPEPVRFLTAASRL
                     GKLSVPVKVAADAFTCPQRVTSGLEAYLGGRQYWVLVKTLAEAGQCIEMLKERRAGRA
                     TFLPLERSRPRFPQSRFSLPKSGVVGWAMDLVTVDREWESAVMHLLGDLLVVERYEDG
                     SALVNKGASFPIVSLDGEVFAPSGTVSGGRARSSAGAIERRSRILESEEKLKSLKRRI
                     SDLTKTLAEEEALERTRAAEKDEWVLGVREAKKEVEEKRRELELERARRDRLASEHAA
                     GLTEASGWERRAGEIDAEMKALMASAAPSMDRGDLSALPAKLSEVEGICALLEERLSS
                     VRALRERADEELARASERGRALLEDESGASRREQEERERLSLWGKEQYRIFLDLREIL
                     GELDNLHGQERALVARSAKISSRSRAAVERAGALRAKADELLRQIGTVSEERQRLIET
                     WEEEYPYDAASAPGPGEGEAIASAVRRLERDLRALGPVDWGALSEDTSLASRVAYLGE
                     QLADVRAAMDELRAIIAETDRHVGVVFTGALDNINVRFDALFRRLFGGGEARLQLLSP
                     PQPGQPDANGEDEAQPEQGESAAAWDRGVEIVARPPGKHLQNLAQLSGGEQTLTAIAY
                     LFASMEVAGVPLAVLDEVDAALDESNLLRFGELAREYACPSEEASNADAEDAGRAASS
                     SAIQLIVMTHRRATMERADILYGVTLAEPGLSKVVGIRVEDWVEPGTDPRGRRAPTAV
                     SLRGRP"
     gene            4250..5032
                     /locus_tag="PK849_00495"
     CDS             4250..5032
                     /locus_tag="PK849_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPE64628.1"
                     /translation="MSAPENAAHPVSRDELLMGSLSLDQPEKGPRVNADTILLASYVR
                     NTFSGARGGSDVLEIGCATGAVSLILAYRFPEIPRIVGVDIQGRLVQLAAANAVRNGF
                     ADRVSFIEADVRAARSSFPAQSFDVVAANPPYESPGRGRLSATHSERLARQEVSCTLE
                     EIAAACRYLLRNRGRLYLVFKAERACELFARLSEQGIEPKRVRWVHPLPGRKASVVLV
                     EALRGGRPGMIVEPPLFIHDATGGYTEEFLSAYTKEGLPCCV"
     gene            5020..5850
                     /gene="rsmI"
                     /locus_tag="PK849_00500"
     CDS             5020..5850
                     /gene="rsmI"
                     /locus_tag="PK849_00500"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPE64629.1"
                     /translation="MLCIVPTPVGNLEDMTPRGVRMLAEADFIACEDTRTSMPLLRRY
                     GISKPLIAYHAFNEKGKTEALVERLLRGEKGALISDAGTPGISDPGYELIRRCIDEGV
                     EMTALPGPTAFVPALILSGLPPYPFLFYGFLPDKQGERERAILSVASVPWTLLFYVSP
                     HKAEKHLLHICALLGDRRAALVREISKIYEEARRGVLSALAESVRGGVKGELVLVVEG
                     SSGERGPSAELPEWRERAASLASEGVSSKEVVKIVSEEYSVPKNEVKSFLFQESGGNQ
                     "
     gene            5916..7895
                     /gene="metG"
                     /locus_tag="PK849_00505"
     CDS             5916..7895
                     /gene="metG"
                     /locus_tag="PK849_00505"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPE64630.1"
                     /translation="MKEQKFAGGIANMNEKKSFYLTTPIYYVNDIPHIGHAYTTIAAD
                     AMCRYKRMKGFDVFFLTGTDEHGQKIQQSAAAKGLTPRELADRTVRNFQDLWKALNIS
                     NDDFIRTTEERHYKTVQSIFKKLLDQGDIYKGKYEGWYCVPCETYVPESSMGEDKTCP
                     DCKRPLQMMEEESYFFRASKYVDQLLAYYEKNLKGVMPRSRYNEIISFLKGGVRDQSI
                     SRTTLKWGIPVPGDDAHVVYVWFDALINYVTACGYLDDRKKFDTFWPEVRHLVGKDII
                     RFHCVIWPIMLLALGVNPPVSVFAHGWWTVEGEKMSKSKGNVVDPFEMVERYGADPFR
                     YFLLREVPFGLDGDFSESAVVGRINSDLANDLGNLLNRTLQMIDNFFQGSLPASCRRG
                     EREEEIVQRAAAVLAEVDEKIEDFAFDDALKSIWSFISRGNKYIDETMPWKLAKEGKT
                     EELSSVLNTLYDILRLSAMLVAPFIPETAARIWEQLGLDGEPVNMRFDGFSWGDRAPG
                     HTVKKGKVLFPRIDLEEWKKAREAKAPAPAQPASEEPAIVHEELVGIDSFKAVELRVA
                     QILNVEEIPKSKKLYKLTVDLGYEKRTIVSGIKEFFTPDELLHKKIVVVVNLQPAKLC
                     GVESNGMLLAAGDGKKTTLALLTPDKDIPLGSRVS"
     gene            8052..8939
                     /locus_tag="PK849_00510"
     CDS             8052..8939
                     /locus_tag="PK849_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPE64631.1"
                     /translation="MKNERDFPQSTAGEERTVPTVPALVDTHCHVHADDYGAETAAVV
                     ERAAQAGVARMLVIGSDIRTSRLAVEAAEQFASSGVFAAVGIHPHEAATEPSCSVEGK
                     GIPEELRGLAAHPRVAAIGETGLDYHYDHSPRDQQRNSFREHIRFSAEAGKPLVIHGR
                     ESYDDLRAILHEERGERIRAVVHCFSGTEDDARDFLEMGYFLSFGGPLTFPKNDALRA
                     LFRELPEERILLETDSPWLTPHPFRGKRNEPMHVRLVYEKAAEIRGVPFASFAAAMES
                     NAARLFGWGTERTLGDRTS"
     gene            8936..10084
                     /gene="tgt"
                     /locus_tag="PK849_00515"
     CDS             8936..10084
                     /gene="tgt"
                     /locus_tag="PK849_00515"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPE64632.1"
                     /translation="MSAKAPLEFVVDAVCPVTGARAGRVITQHGEIPTPVFMPVGTQA
                     TVKAMAPFELEEIGAKIILSNTYHLHLRPGGELVRAAGGLHNFMRWNHPILTDSGGFQ
                     VFSLASLGRISDEGVVCKSHLDGSTHFMSPEWSMETQLHLGSDIAMCFDQCAPYPCSR
                     EEAEEAVRRTTLWAGRSLEAHSRGIGEAPHQALFGIVQGSVHEDLRLRSAAELIPLDF
                     PGYAVGGLSVGEPAEEMYRILDVLNPVLPASKPRYLMGVGRPDNLVEGVARGIDMFDC
                     VLPTRNGRNGTLLTPEGAMNIKKKQYERDFSPVDPECDCYACRTFTRAYLRHLYRAGE
                     ILAMRLCSWHNLRYLVRLGERMRAAILEGTFPEFRREFRARFRAPEGS"
     gene            10084..11091
                     /locus_tag="PK849_00520"
     CDS             10084..11091
                     /locus_tag="PK849_00520"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPE64633.1"
                     /translation="MPPRILPVDPWNPEPGPIAEAAEILLSGGLVAFPTETVYGLGAN
                     GLDPCAAAKIFAAKGRPADNPLILHFAAPDAVDEVALVPSKARRLMELFWPGPLTLVL
                     PSKGTVPPEVTAGLDSVAVRMPSHPAALALLARAKIPVAAPSANSSGRPSPTDAAAVA
                     SDLGDGVDLILDGGETSVGVESTVLDATGEQLVLLRPGGCAVEEIEARSGMKVLLSGG
                     AAHRSPGTRYRHYAPRVPLLLDTDADGIARAAAETDGAPRIAWIGMHAPEISLGEGVA
                     HPGLRILFDSPENYARGLFHALRVFEERGAGLIAAQWPPSEEGIGRALRDRLMRASMK
                     E"
     gene            11169..11256
                     /locus_tag="PK849_00525"
     tRNA            11169..11256
                     /locus_tag="PK849_00525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11203..11205,aa:Leu,seq:cag)
     gene            11387..>13966
                     /gene="rpoB"
                     /locus_tag="PK849_00530"
     CDS             11387..>13966
                     /gene="rpoB"
                     /locus_tag="PK849_00530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPE64634.1"
                     /translation="MAEFVPVSSKRQRLTFGRARDLVEIPDMIEVQRDSYNWFYQESR
                     EADMRTRQGLQELLQEVFPIESYDGSFALEFVRYILDDPSTEEEEARQRDLTWSRPVR
                     ATIRLVNRKTRELKEEEIFLGDFPIMTGRGTFVINGTERVVVNQLARSAGIYFTKDEM
                     IPGQEAYSAKIIPERGAWLEFALSHGEPITVNIDNRKKIPATLLLKIFGAGSNEGVLE
                     LFNARTVEKDVSEDDVRGLLSAESVLDPDGGVILPRNRLITKDHLEALWDMGRTKVRV
                     WDADPALALTLERDNAANADDAMVELFRKLRPNEPARIENAREYVHTLFFDPRRYNLG
                     RVGRYKMNRSLGIAISEEIRLLQLEDLAKIVTGLLTLKEPEKIGDDIDHLGNRRVRSV
                     GELLANQIRIGLLRMERIAKERMTTIPDLGAAMARDLINVRPIAASLREFFGSGQLSQ
                     FMDQTNPLAEVTHRRRLSALGPGGLSRERAGFEARDVHYTHYGRVCPIETPEGPNIGL
                     VTSLATYARLNEYGFLITPRRKVLNGRVTGEIVFLSADEEDGVYVGMANTPVDDEGVI
                     TTAECFTRYQGNIVTVPREEVSYLDVSPKQIVSSSTALIPFLEHDDANRALMGSNMQR
                     QAVPLIAPQAPVVGTGMEHRIAKDSGSCVVARKNGEVEYVDSTRIEIRSGVETIVYPM
                     IKFRRSNQGTIIHQKPLVRKGDSVSAGEIIADGQAVEGGELALGRNVLVAFMAWEGYN
                     FEDAILLSERLVKEDFYSSIHIEEYEIEARDTKLGPEEITRDIPNVGEDVLKNLDDEG
                     IVRVGAEVNAGDILVGKVTPKGESDQTPEEKLLRAIFGEKAREVRDTSLKVPHGARGK
                     VVAV"
BASE COUNT         2675 a         4322 c         4534 g         2435 t
ORIGIN      
        1 gaccgttatc gacgtcaaca ccggtaagta tatcggcaag acggatcttc gccataccgt
       61 cctggagacg aatcttgaag ccgcgtcgga aattgcgcgt cagctcaggc tccgggccat
      121 cggcggcatc gtcgttatcg acttcatcga catggacttt gaagaggacc gttcccgtct
      181 gctcaggagg ctggaggagg tcttccagcc cgacagatac cgcgcgcgcg tttttggcgt
      241 ctcgcagctc ggacttgtag agatcacgcg caaacgagct cgccccgaca tccgctcgat
      301 gctcacccgg agctgccccg cgtgcggcgg aggcggatgg gtcttccgcg aggataccat
      361 cgcgatggct attaagcggt tcctcaggaa ggtctccttc gccaacaggg cggaggcgct
      421 gcttcttgaa gccaacgccg cggttgccca gtatatcttc gagacctacc tcatgctctg
      481 ggaggcggaa ctcggacgga agatcttcat cgccgccgcc ccggacttcg cgtggagcaa
      541 attccgcctt gaggcgcaag ggggcgtcga ccaggtggag cgtcgtatag aacagatgga
      601 gaagcgggag gcacgaatca ttgtatatcg aacgtcttca tcttaaaggc ttcaagagct
      661 tcggagcctc tctggatctc ctcctctcac caggattcac ggcaatcgtc ggtcccaacg
      721 gaagcggcaa gagcaacctg ctcgacgcgc tccggtgggt gctgggcgaa gccggttcgc
      781 aacgcctccg catcgtcaaa cagggggatc ttctcttctc cgggagcgtg acgctgcctc
      841 cggcttcccg cgccgaggtc tctcttctgt tgcgctcgac gccgtactcg ggccgggaga
      901 gtgcgcactc tccggaggcc ggtcagtgcg tgctgcgacg gagctattct tccgaaacag
      961 ggacggtgct caccgtcgac ggaacgcgcg tccggctttc ggatctcgac gaggtgaagc
     1021 gtctctggca tctcgaagga gatcagtttg ccttcatcgg gcagggagag gtcgccgagg
     1081 cgatccatca acgccccgcg cagcgacgcg cgcaccttga ggtgctcttc ggcatcgacc
     1141 agtaccggaa aaagagaaac gaaacgtcgc agaagctcgt cgccgcatcc gaggaggttc
     1201 tgcgtctcgg cgcgctcgcc gcggagttga cgcaccgcag ggaggagatt gctccggccg
     1261 tagaaatcgc gcaagccgcg aagaagatca ccgacgaact ggaagaaaag aggcgggggt
     1321 actacttctt cagacgccgc cttctcgagg agaagtctgc ggccttggga gaagatctgc
     1381 gcacgctcag ggaggaggaa gccctccggc ttcgctggaa agagctgtgg gaggcgatgt
     1441 acacacggtc ggtccaacag tgcggggagc aggaagaacg ggtgaagcgg ctcctcggcg
     1501 accgcgacga actcgttgcg cgacgcgaag gactcaggag gagctgcttt gcaggggcgg
     1561 cctccatacg ggaaatcctg tcgcggcggc gcgccctcgc cgaggaacag aaagagactg
     1621 cggcgcgcct tgcatcgttg acgaaagaga gcgaggagct gaagagcaaa gaggcttctc
     1681 tgcacgtcga gcacggatcg cgtgcttcgg agcgcgaact gctccgaaag aaggtgcgcg
     1741 agctgcacga agcgatcgac gcggagcgca ggcgaaagga cgagaccacg aacacactcg
     1801 cctccctctc cggcgagctg gagacgctcc gttcacgcct tcgctcgcgg cattccttcc
     1861 tggagacgtg cggcgctgac cttgccgcct ccgaagaggc cctgcggact ctggaggagt
     1921 cgcatgccgc agcctcccgg aatctcgaac agcttgagga gagagaaccg gagctcgtag
     1981 aagcccacag cgagattttt gcccgctgcc ggacgaccgg aggaacgctt cagtccgtcc
     2041 gcaaggaggc ctctgcgctc gaagcggcgc tcgaagacct gcggagcgcc gaggactcca
     2101 tgtacccgga gcccgtccgc ttcctcaccg cggcctcaag gctcgggaag ctctccgtcc
     2161 cggtgaaggt ggccgcggat gcgttcacct gtccgcagcg cgtgacatcc ggtctggaag
     2221 cgtaccttgg cggccgccag tactgggtgc tcgtcaaaac cctcgcggaa gccggtcagt
     2281 gcatcgaaat gctcaaggag cggcgcgccg gacgagcgac gttcctcccg ctcgaacgaa
     2341 gccgcccgcg ctttccgcag agcaggtttt ccctgccgaa gagcggcgtt gtcggctggg
     2401 ctatggatct tgtcacggtc gaccgggagt gggagtccgc cgtgatgcac cttctcggcg
     2461 atctcctggt ggtcgagcgc tacgaggacg gctccgctct cgtgaacaag ggggcgtcct
     2521 tcccgatagt ctcgctcgac ggcgaggtgt tcgccccgtc gggaaccgtc agcggcggac
     2581 gcgctcgttc atctgcgggc gcgatcgaac gcagaagccg tattcttgag tcggaggaga
     2641 agctgaagag cctgaagcgg cggatttccg acctgacgaa gacgcttgcc gaagaggagg
     2701 cgctcgaacg tacccgcgcc gcggagaagg atgaatgggt tctcggtgtg cgggaggcga
     2761 agaaagaggt cgaggagaag cgtagggagc tggaactgga gcgggcgcgg cgggaccgcc
     2821 tcgcgagcga acatgcggcg gggctgacgg aagcgtccgg ctgggaacgc cgcgccgggg
     2881 agatcgacgc cgagatgaaa gcgctcatgg cctcggccgc tccctccatg gatcgcggcg
     2941 atctctccgc ccttccggcg aaactctccg aggtggaagg gatttgcgcg ctcctcgaag
     3001 aacgactgtc gagcgtccgc gccctgcggg agcgggccga cgaggagctc gcccgggcct
     3061 ccgagcgagg acgagcgctc ctggaggacg agagcggcgc ttcccgccgc gagcaggagg
     3121 agcgggaacg tctctcgttg tgggggaaag agcagtatcg tatcttcctc gatcttcggg
     3181 agatccttgg ggagctcgac aaccttcacg ggcaggaacg ggcgctcgtc gcccgctccg
     3241 cgaagatctc ctcgcgcagc agagcggccg tcgagagggc gggagcgctg cgtgcgaaag
     3301 ccgacgagct cctcaggcag atcggaaccg tctctgagga gcggcagcgc ctgatcgaga
     3361 cgtgggagga ggagtatccg tacgacgcgg cctccgcgcc cggacccggc gaaggcgagg
     3421 cgatcgcctc cgccgtccgc aggctcgaac gcgatctccg cgcgctcggt cccgtcgact
     3481 ggggagcgct ctcggaagac acgtcgcttg cgtcgcgcgt cgcgtacctg ggagaacagc
     3541 tcgccgacgt ccgggcggcg atggacgaac tgcgcgccat catcgccgag acggaccggc
     3601 atgtcggcgt cgtgttcacg ggcgccctcg acaacatcaa cgttcgtttc gacgcgctct
     3661 tccgcaggct cttcggagga ggagaggcgc gcttgcagct cctttctccg ccccagcccg
     3721 gacagcccga tgcgaacggg gaggacgaag cgcagcccga gcagggagaa agcgccgcgg
     3781 catgggacag aggcgtcgag atcgtcgcac gccctcccgg aaagcatctg cagaatcttg
     3841 cccagctctc cgggggcgaa cagacgctga ccgcaatcgc ctacctcttc gcctcgatgg
     3901 aggtcgcggg ggttcctctt gccgttcttg acgaagtgga cgcggcgctc gacgaatcca
     3961 acctgctgcg cttcggcgag ctggcccggg agtacgcctg tccttcggag gaggcgtcga
     4021 acgccgacgc ggaggacgcc ggccgtgcgg cctcatcctc cgcgattcag ctcatcgtga
     4081 tgacgcaccg gagggcgacc atggagcggg ccgacattct ctacggcgtg acgctcgcgg
     4141 agccgggact ctcgaaagtg gtcggcatac gcgtcgagga ctgggtggag ccggggacgg
     4201 atcctcgcgg acgccgcgcg cctacggctg tttccctccg ggggcggccg tgagcgctcc
     4261 tgaaaacgca gcccatccgg tttcgcggga tgagctcctg atgggctcgc tctcgctgga
     4321 ccagccggag aaggggccga gagtcaatgc agacaccatt ctcctcgcat cctacgtgcg
     4381 caatacgttc tcaggggcgc gcggaggaag cgacgtcctt gaaatcggct gcgccacggg
     4441 ggccgtctct ctcattcttg cgtaccgctt cccggaaatt ccccggatcg tcggggtgga
     4501 catccagggg agactcgtcc aacttgccgc agcaaacgcg gtcagaaacg gcttcgcgga
     4561 cagagtttcc ttcatcgagg cagacgtccg ggccgcccgc tcgtccttcc ccgctcagtc
     4621 gttcgacgtt gtggcggcga acccgccgta cgagagcccc gggagagggc gcctctccgc
     4681 gacgcactcc gaacggcttg cgaggcagga ggtctcgtgc acgcttgagg agatcgccgc
     4741 ggcctgccgt taccttctca ggaaccgggg gaggctctac ctcgtcttca aggcggagcg
     4801 ggcctgtgaa ctttttgccc gtctctccga acaggggatc gagccgaaac gagtgcgctg
     4861 ggtccatcca ctccccggaa gaaaagcctc cgtggtgctt gtcgaggcgc tccggggcgg
     4921 gcgtccgggc atgatcgtcg agccgccgct ctttattcac gacgccacgg gcggatatac
     4981 ggaggagttt ctgagcgcct acacgaagga gggattgccg tgctgtgtat agtccccacc
     5041 ccggtgggaa accttgagga catgacgccg aggggcgttc gtatgctcgc cgaggcggac
     5101 ttcatcgcct gcgaggatac gcgaacctcg atgccgctgc tgcgccgcta cggcatctcg
     5161 aagcccctca tcgcgtacca tgccttcaac gagaagggga agacggaggc gctcgtcgaa
     5221 cgcctcctcc gcggagagaa gggggctctt atctccgacg cggggacgcc agggatctcc
     5281 gaccccgggt acgaactcat ccgcaggtgc atcgatgaag gcgtcgagat gacggcgctc
     5341 cccggtccga ctgccttcgt ccccgcgctc atcctctcgg ggcttccgcc gtaccccttt
     5401 ctcttctacg gttttcttcc ggacaaacag ggggaacgcg agcgggcgat cctctccgtg
     5461 gcctccgtac cgtggacgct ccttttttac gtctcgccgc acaaggcgga gaaacacctt
     5521 ctgcacatct gcgcacttct gggagaccgc agggcggcgc ttgtccgcga gatcagcaag
     5581 atctacgagg aggcgcgccg cggcgttctt tcggcgcttg cggagtcggt gcgcggcggc
     5641 gtgaagggag agctcgtcct cgttgtcgag ggatcgtccg gggagcgcgg gccttcagcg
     5701 gaactgccgg agtggagaga gcgcgccgca tcgcttgcgt cggagggagt ctcctctaaa
     5761 gaggttgtca aaattgtctc ggaggagtat tctgtaccga aaaacgaagt caaatcgttc
     5821 cttttccagg agtccggcgg aaatcaataa cgtaccgcat cgcgggcggc gattcagccg
     5881 gacggcgtga accgttccgc gctcagtccg ttctcgtgaa ggagcagaaa tttgcaggag
     5941 gtatcgcaaa catgaacgaa aagaagagct tctacctcac caccccgatc tactacgtca
     6001 acgacatccc gcacatcggg catgcctaca cgaccatcgc cgccgacgcg atgtgcaggt
     6061 acaaaaggat gaaggggttc gacgttttct tcctcaccgg gaccgacgag cacggacaga
     6121 agattcagca gagcgccgca gccaaggggc tcacgccgcg cgaacttgcc gacaggacgg
     6181 tgcgcaactt ccaggatctc tggaaggcgc tcaacatctc caacgacgac ttcatccgca
     6241 ccaccgagga gcgtcactac aagacggtgc agtccatctt caagaagctc ctcgatcagg
     6301 gcgacatcta taaagggaag tacgaggggt ggtactgcgt cccctgcgag acgtacgttc
     6361 ccgagagcag catgggcgag gacaaaacct gtcccgactg caaacggcct cttcagatga
     6421 tggaggagga gagctacttc ttccgcgcgt cgaagtacgt cgaccagctc ctggcctatt
     6481 acgaaaaaaa tttgaagggc gttatgccgc gcagccgcta caacgagatc atcagcttcc
     6541 tcaagggggg cgtgcgcgac cagtccatct ccagaaccac cttgaagtgg gggatcccgg
     6601 tgcccggcga cgacgcgcac gtggtgtacg tctggttcga cgcgctgatc aactacgtga
     6661 cggcctgcgg ctacctcgac gaccggaaga agttcgacac cttctggccg gaagtgcgcc
     6721 atctcgtcgg caaggacatc atccgcttcc actgcgtgat ctggcccatt atgctcctcg
     6781 cgctcggcgt caaccctccc gtttccgtgt tcgcccacgg ctggtggacc gtcgaggggg
     6841 agaagatgtc gaaatcgaag gggaacgtcg tggacccctt cgagatggtg gagcgctacg
     6901 gcgcggatcc cttccgctac ttcctgctcc gcgaggttcc gttcggcctc gacggcgact
     6961 tctccgagag cgcggtagtg gggcggatca actccgatct tgcgaacgac ctggggaatc
     7021 tgctcaacag aactcttcag atgatcgaca acttcttcca gggatcgctt cccgcatcat
     7081 gcaggagggg ggagcgcgag gaggagatcg tacagcgggc cgccgccgtc ctcgccgagg
     7141 tcgacgaaaa aatcgaggac ttcgccttcg acgacgcgct gaagagcatc tggagcttca
     7201 tctcgcgcgg gaacaagtat atcgacgaga ccatgccgtg gaagctcgcc aaggagggga
     7261 agaccgaaga gctctcctcc gtgctcaaca cactctacga catccttcgt ctgagcgcca
     7321 tgctcgtggc ccccttcatt ccggaaactg cggcgcggat ctgggagcag ctcggacttg
     7381 acggcgaacc ggtgaacatg cgcttcgacg gattctcgtg gggcgaccgg gcgcccggcc
     7441 acacggtgaa gaagggaaag gtcctcttcc ctcgcatcga ccttgaagaa tggaagaagg
     7501 cgcgggaggc gaaagcgccg gcccccgcgc agcctgcttc ggaggagccc gcgatcgtcc
     7561 acgaggagct ggttgggatc gacagcttca aggccgtcga gctgcgcgtg gcgcagattc
     7621 tgaacgtgga ggagataccg aagtcgaaga agctctacaa gctgaccgtc gacctcggat
     7681 atgagaagcg gaccatcgtc tccggcatca aggagttctt cacgccggac gagctgctgc
     7741 acaagaaaat cgttgtggtg gtgaatctcc agcccgcgaa gctctgcggc gtggagagca
     7801 acggcatgct gctcgccgcc ggggacggaa agaaaaccac gctcgcgctg cttacccccg
     7861 acaaggatat ccctctcggc agccgcgtga gctaagagaa cgcatccggg acgccgctcc
     7921 cgcagcgggg agtcccggca ggtatcagtg acggggagcc tctccggaaa tccggggagg
     7981 ctccctgatt cctgtatccg ccgaacactg cggaacagcg tgataacgaa cagaaaatga
     8041 ggatgaagca gatgaaaaat gagagagatt tcccgcagag taccgcgggc gaagagcgta
     8101 ctgtacccac cgttccggcg ctcgtggata cgcactgcca cgtgcacgcg gacgactacg
     8161 gcgcggaaac cgccgcggta gtggagcgcg ccgcgcaggc cggggtggcg cgcatgctcg
     8221 tcatcggaag cgacatacgg acgagccgcc ttgccgtgga ggccgcggaa cagttcgctt
     8281 cctccggggt gttcgccgcc gtggggattc acccgcacga ggcggcgacg gaaccctcct
     8341 gttccgtgga gggaaagggg atcccggagg agcttcgagg attggccgcc cacccccgcg
     8401 ttgcggcgat cggcgagaca ggactcgact accattacga ccattcgccg cgcgaccagc
     8461 agcggaacag cttccgcgag catatccgtt tctccgcgga ggcgggcaag ccgcttgtca
     8521 tccacgggcg ggagtcctac gacgacctgc gcgccatcct gcacgaggag cgcggggagc
     8581 ggatacgcgc cgtcgtccac tgcttctccg ggacggaaga cgatgcgcgg gattttctgg
     8641 agatggggta cttcctctcc ttcggcggcc cgcttacatt tccgaaaaac gatgcgctgc
     8701 gcgcgctctt ccgtgaactg ccggaggagc gtatcctgct ggagaccgac tcaccctggc
     8761 tgactccgca cccgttcagg ggcaagcgga acgagccgat gcacgtcagg ctggtctatg
     8821 agaaggctgc ggagatcagg ggcgttccct ttgcctcttt cgccgccgca atggagtcga
     8881 acgccgcccg cctcttcgga tggggtacgg agcggacatt gggggacaga acgtcatgag
     8941 cgcgaaagct cccctcgaat tcgtcgtcga cgcggtctgt ccggtcaccg gagcgcgagc
     9001 cgggcgcgtg attactcaac acggcgagat tccgactcct gtcttcatgc cggtcggcac
     9061 tcaggcgacc gtcaaggcga tggcgccctt tgaacttgag gagatcggcg cgaagatcat
     9121 tctctccaac acctaccacc ttcacctgcg gcccggcggc gaactcgtcc gagcggccgg
     9181 ggggctgcac aatttcatgc ggtggaacca tcccatcctg acggacagcg gagggtttca
     9241 agtcttttcc ctcgcctcgc tcggccggat ctccgacgag ggcgtggtgt gcaagtcgca
     9301 cctcgacggt tcgacgcact tcatgtcgcc cgagtggtct atggaaacgc agcttcacct
     9361 cggaagcgac atcgccatgt gcttcgacca gtgcgccccg tacccgtgct cgcgcgagga
     9421 ggcggaggaa gctgtgcggc ggacgaccct ctgggcaggc cgctctcttg aagctcattc
     9481 ccgaggaatc ggagaggccc cgcaccaggc cctcttcggc atcgtgcagg ggtcggtcca
     9541 cgaggatctg cggcttcgat ccgccgctga actgatcccc ctcgattttc cggggtacgc
     9601 tgtggggggg ctttcggtcg gcgaacccgc cgaagagatg taccggatac tcgacgtgct
     9661 caatccagtg ctccccgcgt ccaaaccgcg ctacctgatg ggcgtcggca gaccggacaa
     9721 cctggtcgag ggcgtcgccc gcggcatcga tatgttcgat tgcgtgctcc ccacccgcaa
     9781 cggccgcaac ggcacgctcc tcacgccgga gggagcgatg aacatcaaga agaagcagta
     9841 cgagagagac ttctcgcccg tcgacccgga gtgcgactgc tacgcctgcc gcaccttcac
     9901 acgcgcgtac ctccgtcatc tctacagggc aggggagata cttgcgatgc gcctgtgcag
     9961 ctggcacaac ctccggtatc tcgtcaggct cggagagcgg atgcgcgccg ccatcctgga
    10021 ggggaccttc ccggaattcc gcagggagtt ccgcgcgcga ttccgcgcgc cggagggctc
    10081 ctgatgccgc cgcgcatcct ccctgtcgac ccctggaatc ccgaacccgg accgatagcg
    10141 gaagccgcgg agatactcct ctcagggggg cttgtcgcct ttccgacgga gaccgtctac
    10201 ggactcgggg caaacgggct ggacccctgc gccgcggcga agatttttgc cgcgaaggga
    10261 cgacctgcgg ataacccgct catccttcac ttcgccgcgc ctgatgcggt agacgaagtc
    10321 gcgttggttc catcgaaagc cagacgcctg atggagctct tctggcccgg gccgctgacg
    10381 cttgtgcttc cgtcgaaggg tactgtgcct cccgaggtga ccgcggggct cgacagcgtc
    10441 gccgtccgga tgccgtcgca cccggcggcc cttgcgctcc ttgcgcgggc gaaaataccc
    10501 gttgcggcgc cgagcgcgaa ctcgagcggc aggccgagcc cgacggacgc cgccgccgtg
    10561 gcgtcggacc tcggggatgg agtcgatctc atcctcgacg gcggggagac gtcggtaggc
    10621 gtcgaatcca ccgtcctcga cgccaccggc gagcagctcg ttctccttcg tcccggaggg
    10681 tgcgcggtcg aggagataga agcccgctcc ggcatgaagg tgctcctctc cggcggcgcg
    10741 gcgcacaggt cgccgggaac gaggtatcgt cactacgccc cccgcgtgcc gctgctgctg
    10801 gatacggacg cggacggaat cgctcgcgcc gcggcggaaa cggacggagc gccgcgcatc
    10861 gcgtggatcg gcatgcatgc accggagatc agtctgggtg agggtgttgc gcaccccggg
    10921 ctgcgtatcc tcttcgactc cccggagaac tacgcgcgcg gccttttcca tgcgctcagg
    10981 gtctttgagg agaggggagc cggtcttatc gcggcgcagt ggcctccctc ggaagaaggg
    11041 ataggacgcg cgctgcggga cagattgatg cgggcttcaa tgaaagagtg attttttccg
    11101 aggaattctt ttttcgctct tgcaaaagcc ttcttccctt gctacaatta cccgcgtggt
    11161 tttgaaaggc cggggtggcg gaattggtag acgcgctaga ttcaggttct agtaggcata
    11221 acgcctgtcg gggttcgagt ccccgcttcg gcaccatcga aaatttgagg gcaacctttt
    11281 ttcgaaggtc gtactatgtc tgttgacata gtacgacctt tttgttatct taactctttg
    11341 tcagcaccat tttccccatt tttatttcag ggaggtcaaa aggttcatgg ccgaatttgt
    11401 ccccgtcagc agcaagcgcc agaggttgac ctttggacgt gcccgggatt tagttgaaat
    11461 ccccgacatg atcgaagtgc agcgtgattc ctacaactgg ttttatcagg agagcaggga
    11521 agcggacatg cggacccggc agggattgca ggagctgctg caggaagttt ttccaataga
    11581 aagctatgac ggctcgtttg ccctcgagtt cgtccgctac atactcgacg atccttctac
    11641 ggaggaggag gaggctcgtc agcgggatct gacgtggtcc cgtcccgtcc gtgcgacaat
    11701 ccggcttgtg aaccggaaaa ccagagagct caaggaagag gagatctttc tcggcgactt
    11761 tcctatcatg acggggagag gtacgttcgt catcaacggg acggagcgtg tcgtcgtgaa
    11821 ccagcttgcg cgttctgcag gaatttactt cacgaaggac gagatgattc cggggcagga
    11881 ggcgtactcc gccaaaatca tccccgagcg gggagcctgg cttgagttcg cgctctccca
    11941 cggcgaaccc atcaccgtga acatcgacaa ccggaagaag attccggcga cgctgctgtt
    12001 gaagatattc ggcgccggct cgaacgaggg cgtgctcgaa ctcttcaacg cgcgcaccgt
    12061 ggaaaaggac gtctcggaag acgacgtcag agggctgctt tccgccgaat ccgtgctcga
    12121 tccggacggc ggcgtgatcc tcccgaggaa ccgcctgatc acgaaagatc atctcgaagc
    12181 gctctgggac atggggagga cgaaggtccg cgtctgggac gccgaccccg ccctcgcgct
    12241 cacgctggag cgggacaacg ctgccaacgc cgacgacgcg atggtggaac tcttcaggaa
    12301 gctgcgcccg aacgagcccg cacgaataga gaacgcacgg gagtacgtcc atacgctctt
    12361 cttcgatccg aggcgttata acctgggccg cgtggggcgc tataaaatga accggagcct
    12421 cggcatcgcg atttccgagg aaattcgcct gctccagctc gaagacctgg cgaagatcgt
    12481 cacaggtctc ctgacactga aggaaccgga gaagatcggc gacgacatcg accatctcgg
    12541 caaccggcgc gtccgctccg tcggcgaact gctcgccaac cagatacgca tcggcctgct
    12601 gcgcatggag cgcatcgcca aggagcgcat gacgacgatt cccgacctcg gcgcggccat
    12661 ggcgcgcgat ctgatcaacg tgcgccccat agccgccagc ctgcgcgagt ttttcggttc
    12721 ggggcagctt tcgcagttca tggaccagac gaacccgctc gcggaagtga cgcaccgccg
    12781 ccgcctctcc gcgctcggac cgggagggct ctcccgcgaa cgcgccggat tcgaagcgcg
    12841 cgacgtgcac tatacgcact acggcagggt ctgcccgata gaaacgcccg aaggcccgaa
    12901 tatcggtctt gtgacctcgt tggcgacgta tgcccgcctg aatgaatacg gctttctcat
    12961 cacgcctcgc cggaaggttt tgaacggccg cgtcacgggg gagattgttt tcctttccgc
    13021 cgatgaagag gacggcgtct acgtcggcat ggcgaacact cccgtcgacg acgagggggt
    13081 tatcacgacc gccgagtgtt tcacgcgata ccagggaaac atcgtcacgg ttcctcgcga
    13141 agaggtgagc tacctcgacg tgtcgccgaa gcagatcgtt tcgtcctcta cggcgctcat
    13201 ccctttcctt gagcacgacg acgcgaaccg cgccctgatg ggctcgaaca tgcagcgtca
    13261 ggcggtgccg cttatcgcgc cgcaggcgcc tgtggtcggc acggggatgg agcaccgcat
    13321 cgccaaggat tcgggctcgt gcgtcgttgc ccgcaagaac ggagaggtcg agtacgtcga
    13381 ttccacccgc attgagatac gaagcggggt ggaaacaatc gtctacccga tgatcaagtt
    13441 ccgccgttca aaccagggaa cgatcattca ccaaaaacct ctcgtccgga agggcgattc
    13501 cgtctccgca ggggagatca tcgcggacgg ccaggccgtc gaaggcggcg aactcgcgct
    13561 tggacggaac gttctggtgg ccttcatggc ctgggagggg tataacttcg aggacgctat
    13621 cctgttgagc gagcgtcttg ttaaagagga cttctactcc tcgattcaca tcgaggagta
    13681 cgagatagag gccagggaca cgaaactcgg tcccgaggag attacccgcg acatcccgaa
    13741 cgtcggcgag gacgtgctga agaacctcga cgacgagggg atcgtccgcg tcggcgcgga
    13801 agtgaacgcc ggagacatcc tcgtcggcaa ggtcaccccc aagggggagt cggaccagac
    13861 gcccgaggag aagctccttc gcgcaatctt cggcgagaag gcgcgcgagg tgcgcgacac
    13921 ttcgctgaaa gtgcctcacg gcgcgcgagg caaggtggtc gcggta
//
LOCUS       DAOPKU010000008        13901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_4617_length_13901_cov_5.083273, whole genome shotgun sequence.
ACCESSION   DAOPKU010000008 DAOPKU010000000
VERSION     DAOPKU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13901
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4617_length_13901_cov_5.083273"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PK849_00535"
     CDS             <1..529
                     /locus_tag="PK849_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HPE64635.1"
                     /translation="KGLAAFLGSTLGILLTCGLSLYFGGEFRIHGAVKPFSETLLYSG
                     FAHLDLTRIFLGGIFLASSGAVMDLAMDVASAMREIVEKKPDITRKALIMSGFTVGRA
                     VVGTMTTTLLLAYTGSYTSLLLVFVAQGIPLANILNMQYVSAEILHTLVGSFGLVTVA
                     PFTALASGFLFVKKS"
     gene            complement(760..1461)
                     /locus_tag="PK849_00540"
     CDS             complement(760..1461)
                     /locus_tag="PK849_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64636.1"
                     /translation="MDKKTELIHRLRVDFLSKYGSSELYHEMDSVVNEMTGYLSHLLN
                     TKEDIELFFQCLPQILSEMPRESEETPLGHAPSYSAGVFETDARNAVSMSAFLKPFIR
                     ESVEEAGSLAYFHEYHPRTDNQPAHSNEGAKLQAVFNFQGVEELKKLCVRTGVNYERL
                     RKECCYHGEIHGVPVFVSYALDESSKKGRLELNILDPENPYTITETSRAMARNVDSFL
                     KAVFNKKKRRFANKS"
     gene            complement(1752..2885)
                     /locus_tag="PK849_00545"
     CDS             complement(1752..2885)
                     /locus_tag="PK849_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HPE64637.1"
                     /translation="MLKYFVADAFADEVFEGNPAGVCILDEWLSEDLMKKIAMENNLS
                     ETAFAVKEDERTYGLRWFTPVGEIDLCGHATFGTAFVLFRFEEQTADTLHFHAKKCGH
                     HLIVTKNGDMLAMRFPTIAPERYAYAEYMGEGMGAVPSEVYRTERDLLLVYDSEETIR
                     NMKPDFAKLKEFPVGLSVYVTAKSSGNDYDIVARAFWPKINVNEDPVCGTMYCTLMPF
                     WRERLGKDTLVARQLSARGGTVYCSYNGDSVGISGRGALYSVGTIFVNGSNEYSISKI
                     NTTDTAELCEISVENKRALGISVNIPGTELQILQIRCQKGMLFCRVFDTSVVEKLQFP
                     AAIFSAPKFEDMLNNNPIHLSKAAVALGATADMTGRELVKLFS"
     gene            3156..3515
                     /locus_tag="PK849_00550"
     CDS             3156..3515
                     /locus_tag="PK849_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPE64638.1"
                     /translation="MEKKRKFNCTIEASMCFIGGKYKPLILWHLIEGTKRFSALQRLL
                     PQATPRMLTMNLRELESDGLVHRKVYPEVPPKTEYSLSDLGKTLIPLITELREWGRYY
                     FEVTGQPNPCDVPSAEK"
     gene            complement(4081..5148)
                     /locus_tag="PK849_00555"
     CDS             complement(4081..5148)
                     /locus_tag="PK849_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE64639.1"
                     /translation="MAAHLKDEDLKPEEREEYLSILVKSGESLLDILNNILDIARLES
                     GSAREERSSFRPAVLAQEVVALFAHRAAEKGIALTPFVDPNLPEVLLGYPMRLKQVLF
                     NLVGNGIKFTDSGEVTISLGCARRSGDETLLAVAVKDTGIGISPKGIERIFTPFSQAD
                     DSISRRYGGTGLGLAISKRLVEFMGGRIAVASKLGEGSVFSFTLPVTVGAEDVAAETN
                     SLNAGETPLRGLSVLVAEDNVFNRKVLEKILEKSGISFDSAVNGVEAVKKGTEGAFDA
                     ILMDIEMPEMDGYEASRAIRRSEKETGRRVPIIALSAHVSEEHRQMAFEAGMDAFASK
                     PVRKTDLLRALAEATGREEAP"
     gene            complement(5133..6170)
                     /locus_tag="PK849_00560"
     CDS             complement(5133..6170)
                     /locus_tag="PK849_00560"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HPE64640.1"
                     /translation="MHWKRHLPVLAVLTALLWYGANHNYLLFHSIVEFADITIYLFAA
                     FLGVFASRMTPDPFLYGVSVVYFAMALVTAVHTLAFEGMGVFPGWTANHPTQLWMLMR
                     FIHGIGVPLTLLFSSRPQFIRFFTAGAMLSVFGGILLIAAGYFPDCFIPGEGLTLFKV
                     AGEYAVALLLVLSILYSLAYRRREARRYGYSFEISLACFIASGMAFTLYTDVYGFFNM
                     LGHILHGYGAYILLFGFVVKSAQTLMDRHFYDLNEQIRNMNRELENRVAQRTRELEET
                     NALLASDIARRKEAEEHLRTAKEEAEEANRAKSAFLATMSQREASRSKRPSEGTKSAR
                     RSTAFSAWRPT"
     gene            6390..7550
                     /locus_tag="PK849_00565"
     CDS             6390..7550
                     /locus_tag="PK849_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPE64641.1"
                     /translation="MSIYMNNGATTWPKPESVARAMYDFLTGGGANVARGSASKRDLK
                     SMDTVLSCREELVSLFGGWDDDPRYATFTANVTESINVVLKGFLKPGMTVLTSSMEHN
                     AVLRPLRRMETRGVKVEIAPCDEEGFLHPDIFAEALSRTRPDLVILSHASNVCGTVQD
                     LSALAPLCRDAGVPFAVDSAQTGGILPISLRELGLAALCFTGHKGLLGPQGIGGIVWN
                     PEFAQKVDWFVEGGTGSFSHLEFQPDAMPDKFEAGTPNLPGIAGLLAALSWLGETGIN
                     AVRAREEALGTRLLNGLLTLPGVRLSGRRTMEGRLPVFAVNFEGRDNGLVAGRLSEEF
                     GIETRPGLHCAPLAHKTLGTFPGGALRLSPGYFSTEEDVDATLSALRTILDS"
     gene            7740..9104
                     /gene="trkA"
                     /locus_tag="PK849_00570"
     CDS             7740..9104
                     /gene="trkA"
                     /locus_tag="PK849_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPE64642.1"
                     /translation="MKIVVVGGGEVGYNVANTLSEEEHDVTVVEEDEERAEKVDNELD
                     VITVRGSGARPQVLEEAGIFPGCDVDILVACTDRDEVNILACWIAKRAGVKRVISRAR
                     ALEFTDSPTWAKDLGIDVMNSPERSVAREILELLFVSSAVHTAELLDGRSGIYALRVS
                     PNSALVGLALKDLRTLYPNFIAIIVYIERDGTGYVPYGEMVIEANDLCYIVTSREQAW
                     KLEEIYQMKKSRPLKRVLIIGGGKLGFQVAWKLESQFRNLDIRLIDHNREKCERLASE
                     LKRTLVLCGDGADESLLRQEGIEGADGLVCATESDEANLLFAVIGKALGARKTIAVVR
                     RKIYMKLDNYMSVDAIVNPNSALASVILRHARYKSGAGVLSIIEKIDAEMLELTVPAA
                     SPAVGRTIMSLGMPKGMLVALIVRKGDVFVPVGATVVQADDKVIIFATSDLMPEAMDY
                     LGVQ"
     gene            9107..10558
                     /locus_tag="PK849_00575"
     CDS             9107..10558
                     /locus_tag="PK849_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPE64643.1"
                     /translation="MHLRLVSKVLSLLSAIVSLSMLWPLVWSLWDGTPDVRSFLLSIA
                     FGLALALVLYAFGKQADYDELGIKDGFVAVSLAWVIASVVGAMPYWLHGTVPTFTDAF
                     FEAMSGFTTTGASVLSDIEANPRGILFWRDLTHWLGGMGIIVLSLAILPFIGVGGMQL
                     YKAEVPGPIPEKMTPRIQQTALYLWGVYVLLSALQTALLMLGGMNLFESLTHTFGTMA
                     TGGFSPLNKSIGQYNSAYFDWVITIFMFLAGVNFVLHYRFLLGRWGAFWRDEEFRFYT
                     VLTVFCTLTVTFVLRLHGTYDSFVDALRFGAFQVVSIITTTGYVTADYELWPTYTQFI
                     LLLLMFVGACAGSTGGGIKNLRRMVLARHVRAELSSILHPKAIVHVRVGGKPVGREII
                     ASVTSFFILYIAIFTAGTLFMTILDLDLITAMSSVAATLGNIGPGLGDVGPMQNYAAI
                     PDSGKWVLSLCMMMGRLELYTVVLLLLPETWKR"
     gene            10640..10963
                     /locus_tag="PK849_00580"
     CDS             10640..10963
                     /locus_tag="PK849_00580"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE64644.1"
                     /translation="MKLIDMNRLENEQILPGLRARFVNAENVSFAQWTMEAGTFFPLH
                     DHAHEQILNVISGKVEMNVAGEKVILDEGCALVLAPGEPHAMKVLTDTFMVGVYHPRR
                     DGYKK"
     gene            11084..13072
                     /locus_tag="PK849_00585"
     CDS             11084..13072
                     /locus_tag="PK849_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE64645.1"
                     /translation="MTDETYRAMPLPFSSSSGCTEEALRESEENFRTFFEAIDELASV
                     ISLDGNILFTNGAFRRKTGYAEDEIVGMSALDLELPENREKGAEVFAAMLRGELKSCS
                     MPMRAKDGSHVLVETRLWFGRWNGAPCVYTLSKDVGPEQEAQQRFEAVFRNNPAMMVL
                     ASLPDQRCVDANTTFLRTLGYEKEDVVGKQAREIGLFIDPEQQREIGEMMRNDGRITD
                     YEVHAKNRNGDVFHGLMSADRISIHGRPYLLTVTVDITVRKRAEEALLVAKEQAEWLG
                     RQAEAANRAKSAFIANTSHEIRTPLNGVVGFLDLLARTRLDDVQREYVEGIRASAGSL
                     LDVINDVLDISKIEADKVELRPAPTDIRELAGQAADTVLGTARTKGLHLTVNVAPEVP
                     GAFFLDQSRLKQVLINLLGNAVKFTHEGGVSFSIRCEEAEEDRCLLRFSVEDTGIGIR
                     PESMERLFEPFFQADVSNTRKYGGTGLGLSICSALLQMMGSELKVESVPGEGSRFFFD
                     LRAERVPVSETTEEERARIVPEEPFMPATRPTILIIEDEELNRRLLRLLLSKLLDADI
                     VEAEGGEEGLERFRERAPDLIFLDLQMPGRDGHWTARTLRELEGAGVHTPIVAFTADV
                     QPATREACFASGMDDYICKPADLYTLREVLERHLKTKE"
     gene            13138..>13901
                     /gene="murQ"
                     /locus_tag="PK849_00590"
     CDS             13138..>13901
                     /gene="murQ"
                     /locus_tag="PK849_00590"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPE64646.1"
                     /translation="MIELKHLTTEQRNEKTLDLDSMPLDEALRVMNAEDAKVAPAVGE
                     QLPAIAKAVEAVVRSFESGGRLLYLGAGTSGRLGILDATECVPTFGTPPEMVVGRIAG
                     GMGALVRSVEGAEDSLTMGVDDLKELKADSRDTVVGIAASGRTPYVIAALEYAKSVGC
                     ATVGVTCNKNSAVGAAADIAIEIDVGPEVLTGSTRLKAGTAQKLALNMISTMAMVGIG
                     KVYKNLMVDVQRTNEKLRTRAVNIVADAAEVDKETAR"
BASE COUNT         2668 a         4118 c         4358 g         2757 t
ORIGIN      
        1 gaaagggctg gcggcgtttc tggggtcgac gctgggcatc ctgctcacct gcggactctc
       61 gctctacttc gggggggagt tccgcatcca cggagcggtg aagcccttct cggagacgct
      121 gctctacagc ggcttcgcgc acctcgacct gacgaggatc ttcctcggtg gcatctttct
      181 ggcctcttcg ggggcggtga tggacctggc gatggacgtc gcgagcgcga tgcgagagat
      241 cgtggagaag aagccggaca taacgagaaa ggcgctgatc atgtcgggat tcaccgtggg
      301 gcgcgcggtg gtcggaacga tgacgacgac gctgctgctg gcgtacaccg ggagctacac
      361 gagcctgctg ctggtcttcg ttgcgcaggg gatcccgttg gcgaacatcc tgaacatgca
      421 gtacgtctcg gcggagatac tgcacacgct ggtggggagc ttcggcctgg tgacggtcgc
      481 ccccttcacc gccctggcga gcggttttct gttcgtaaaa aagagctgaa atccgacgcg
      541 ctcagtccgt gcatcggaga gcgtgtcttg gcgtaagtcg agggaggttt cggaaaaggg
      601 aagcggaagt acgaacagga gcggatgcgg ggagcgcttc ccgggagagt cgccgtatca
      661 tagtccttca gttggcccca atgaatcctt ggcgtgccgg tcatgtcgcg cgcgatcctc
      721 ttctgcaacg tatccgcttc ggtttcgacg aaacacggtt catgacttgt tcgcgaagcg
      781 ccgcttcttc ttattgaaaa cggcttttaa gaaactgtcg acatttcggg ccattgcgcg
      841 cgaagtttcg gtgattgtat atggattctc agggtcaaga atgttcagtt caaggcgccc
      901 ctttttgctg gactcgtcca aagcgtacga cacgaaaaca gggacgccgt gaatctctcc
      961 atggtaacag cattcttttc tcagtctttc atagttcacc cctgtcctga cgcacagttt
     1021 tttcagctct tcaacaccct gaaagttgaa aacggcctgt aatttggcgc cttcattcga
     1081 gtgggcgggc tgattgtccg tccgcggatg atactcatga aaatatgcaa gactcccggc
     1141 ttcttccacg ctctcacgaa tgaacggttt gagaaatgcg ctcatcgaaa ccgcgtttcg
     1201 agcgtccgtt tcaaaaacac ccgccgaata ggagggggcg tgcccgagcg gagtctcttc
     1261 gctctcgcgg ggcatttccg aaagaatttg cggaaggcat tgaaaaaaga gttctatatc
     1321 ttcttttgta ttcagcaagt gggagagata gcccgtcatt tcgttgacaa cggagtccat
     1381 ctcatgatag agttccgaac ttccgtattt tgaaagaaaa tcgacgcgaa gccggtgaat
     1441 caattccgtt tttttatcca ataccctgcc ccccttcagg actctcttcg cgaaagccgt
     1501 tttacttttt gattcagaat atcctgcaga gcaagcgtcg ttctccgagc gagcaagcag
     1561 agtatcgttt cgtttcggaa caaatgcaag agtatatgcg ctgtgcccgg gcgctattgc
     1621 aagccctggg cggtgaaggc atgggtttga cgcgctctcg ggcggccgtc ccttcgcctc
     1681 atttcaacag gaaattccat ttcgctcaaa tacgaatgtg ttgttcccgt ccggttttgt
     1741 agagcgaccg ctcaactaaa cagcttcacc aactcgcgtc cggtcatatc ggctgttgcg
     1801 cctaacgcta cggcggcctt gctgagatgg atggggttgt tgttcagcat gtcttcgaac
     1861 ttgggagcgg aaaagattgc cgccgggaat tgaagttttt cgacaaccga cgtatcgaaa
     1921 actctgcaaa aaagcatccc tttttggcat cgaatctgta gaatttggag ttcggtaccg
     1981 ggaatgttta cggaaatgcc cagcgcgcgc ttgttctcca cggaaatttc gcagagttcg
     2041 gctgtatccg tcgtattgat tttcgatatg gaatactcgt tcgatccgtt cacaaaaatg
     2101 gttccaacgg aatagagcgc gcctcttccg ctgatgccga cgctgtcgcc gttgtacgaa
     2161 caatagacgg ttccgcctcg cgctgaaagc tgccgcgcca ccagcgtgtc tttcccaagc
     2221 cgttcgcgcc agaagggcat cagagtacag tacatcgttc cgcacaccgg atcttcgttg
     2281 acgttgattt tcggccagaa tgcgcgcgct acgatatcgt agtcgttgcc gctgctcttc
     2341 gcggtcacat agaccgagag accgaccggg aattccttga gcttcgcaaa gtcgggcttc
     2401 atatttcgga tcgtctcttc ggaatcgtac accagcagca agtcgcgctc cgttctgtac
     2461 acctcggagg gaacggcgcc catgccctcg cccatatact cggcataagc gtatcgctcg
     2521 ggagcgatgg ttggaaagcg catcgcgagc atatcgccgt tctttgtaac gatcaggtga
     2581 tgaccgcatt ttttcgcgtg aaaatgcaac gtgtcggccg tttgttcctc gaagcgaaac
     2641 agaacgaagg ctgttccgaa ggtggcgtgt ccgcaaagat cgatctcccc gaccggggta
     2701 aaccagcgca ggccgtatgt acgctcgtct tctttcaccg cgaatgcggt ttccgacaga
     2761 ttgttttcca tggcgatttt tttcatcagg tcttccgaaa gccactcgtc aaggatacac
     2821 acgcccgccg gattcccttc gaagacctcg tcggcgaagg catccgcaac aaaatacttc
     2881 agcatcgttt ttcctccttc cagatattcg cacgcgatcg tttcactcca gaatccccga
     2941 tgagagcgga ccgccatgcc ggtcgtaccg gcatccgcga atccggggag cgttttgaaa
     3001 tacatcggag atacttgtcg cgagcgcagg catctgcggc gactccttcg ccgtgtttga
     3061 tacgtccgcc tttttactat aggataatga tatggaagac gcaagtacgc acatttctta
     3121 taggtactat caaaaactat agcgggggta tcctcatgga aaaaaagaga aaattcaact
     3181 gtacgatcga ggcgtcgatg tgcttcatcg gcgggaaata caagccgctg atcctgtggc
     3241 atctgatcga gggaacgaag cggttttccg ctctgcagcg gcttcttccg caagcgacgc
     3301 cccgtatgct gacgatgaat ctgcgggaat tggaatcgga cggtctcgtt caccgaaagg
     3361 tatacccgga ggtgcctcca aagaccgaat actctctctc ggatttggga aagactctga
     3421 tcccgttgat cacggaatta cgcgaatggg gaaggtatta tttcgaggtt acgggacagc
     3481 cgaacccgtg cgatgtgccg agcgcagaga agtaaatttc caaggtgtcg ctgaatcctt
     3541 tgcccgctcg atctccgtat tgattccctt aaaagcacct ttcatgctcc tcatagggcg
     3601 aatcggcacg agaacgattt tttcgtcgta cgacagaacc tccagtcgag tccctggctt
     3661 tgcgccgatc gcgtctccga tctcctttgg gattactatc tggtactcgt tggaaatagt
     3721 gactgtattc attcgcccgg gcctcccttc gtgagagccg ttttacttgt gattcagaat
     3781 accctgcaga atatgcgtcg ttctctgacc atgcaagcat agtatcgttt cggaacaaat
     3841 gcaagagtat atgcgacgtg cccgggcgtt attgcaaacc ctgggcggtg aaggcatggg
     3901 tttgacgcgc tctcgggcgg ctggctcttt ttcgggaagg aaggccgctc atggatatgc
     3961 acgccgtcgt ctggaaggct gttgattcca tccgctcgaa ccgtgtcgaa gaccttgacg
     4021 tcgagaccgt cgccgaggcg gtgaactcca cgaggttccc atttcgaggg aggaacctct
     4081 ctacggggct tcctctctcc ccgtcgcctc cgcgagggcg cgcagcaggt cggtctttcg
     4141 caccggctta cttgcgaacg cgtccatccc cgcttcaaaa gccatttgcc gatgctcttc
     4201 cgacacgtgc gccgaaagcg cgatgatggg gacgcggcgt cctgtttcct tctcactgcg
     4261 ccggatggcc cgggaggcct cgtagccgtc catctccggc atctcgatgt ccatcaggat
     4321 ggcgtcgaac gcgccttccg ttcctttctt caccgcttcg acgccgttca ccgcggagtc
     4381 gaacgagatc cccgactttt ccaggatctt ctccagcacc ttccggttga agacgttgtc
     4441 ttcggctacg aggaccgaaa gaccgcgtag cggcgtctcc cctgcgttga gggagttcgt
     4501 ctccgcggca acgtcttccg cgccgaccgt taccggcaga gtgaacgaaa agacgctccc
     4561 ctcgccgagc ttcgaagcga cggcgatccg ccctcccatg aactcgacaa gacgtttcga
     4621 aatagccagt cccagccccg tcccgccgta gcgccttgag atggagtcgt cggcctggct
     4681 gaagggcgta aaaatcctct cgatgccctt cggagatatg ccgatgccgg tatccttcac
     4741 ggcgacggcc agaagcgtct cgtctccgga gcggcgcgcg cacccgaggg aaatggtcac
     4801 ctccccgctg tcggtgaact tgattccgtt gccgaccagg ttgaagagca cctgcttcag
     4861 gcgcatcggg tagccgagca ggacttcggg cagattcggg tcgacgaatg gcgtcagagc
     4921 aatccccttt tccgccgccc ggtgtgcgaa gagtgcgacg acctcctggg cgagaacggc
     4981 gggccggaaa gaggatcgtt cctcccgcgc gcttcccgac tccagccgcg cgatgtcgag
     5041 gatgttgttc aggatgtcca gcagcgactc ccccgatttg acgaggatgg agaggtactc
     5101 ttcccgctct tccggcttca gatcctcgtc cttcaggtgg gccgccatgc cgagaatgcc
     5161 gttgagcggc gtgcggactt cgtcccttcg ctcggacgct tgctccggga ggcttcgcgc
     5221 tggctcatcg tggcgaggaa ggcgctcttc gcccggtttg cctcctccgc ttcctccttg
     5281 gccgtgcgga gatgctcctc ggcctccttg cgccgggcga tgtccgaggc aaggagcgcg
     5341 tttgtctcct ccagctcgcg cgttcgctgc gcgacacggt tttccagttc gcggttcata
     5401 ttccgtatct gctcgttcaa gtcgtaaaaa tgcctgtcca tcagcgtttg ggccgacttg
     5461 acgacgaagc cgaagagcag aatgtaggcg ccgtagccgt ggagaatatg ccccagcata
     5521 ttgaagaagc cgtagacgtc cgtgtagagg gtgaaggcca ttccgcttgc gatgaagcag
     5581 gcgagcgaaa tttcgaagga gtagccgtat cgccgcgcct ccctgcggcg gtacgcgagc
     5641 gaatacagga tggaaagaac aagcagcagc gcgacggcgt actcgcccgc caccttgaag
     5701 agcgtgagtc cttcgccggg gatgaagcag tccgggaagt atcccgccgc gatcagcaga
     5761 atgccgccga agaccgagag catcgcgccc gcggtgaaaa aacggatgaa ctgaggacgc
     5821 gaggagaaga ggagcgtcag cggaacgccg attccgtgaa tgaagcgcat cagcatccag
     5881 agctgcgtgg ggtggttcgc cgtccacccc gggaagactc ccattccctc gaaggcgagc
     5941 gtgtggacgg ccgtgacgag cgccatggcg aagtagacga cgctgacgcc gtagaggaaa
     6001 ggatccggcg tcattcgcga ggcgaagact ccgagaaagg ccgcgaagag atagatcgtg
     6061 atgtcggcga actccacgat cgagtgaaag agcaggtagt tgtgattcgc tccgtaccag
     6121 agaagcgctg tcaggacggc gagaaccggc agatgacgtt tccagtgcat agaaaatcct
     6181 ccttggtacg gcaaagaagg caggggggta tcttctgaca ctgtctccac cgtattgcca
     6241 gtgaaagtat agcattgttt cccacgaatc gctccgggaa cgcgccgttc tttacccgtt
     6301 gcagccgcat ctttcccccg aagtctggcg tttttgcaga aaagcagtac aatggtgcat
     6361 atattttata ataaggaagg gtgatcgcta tgtccattta tatgaacaac ggggcaacaa
     6421 cgtggccgaa gccggagagc gtggcgcggg cgatgtacga ctttctgacg ggaggggggg
     6481 cgaacgtcgc gcgggggagc gcctcgaagc gggatctgaa gagtatggac accgtcctct
     6541 cctgcaggga ggagcttgtt tcgctgttcg gcggatggga cgacgacccc cgctacgcca
     6601 cgttcacggc gaacgtgacg gaatccatca acgtcgtcct gaagggcttt ttgaagccgg
     6661 ggatgaccgt gctcacgtcg agcatggagc acaacgcggt gctccgcccc ctgcgcagga
     6721 tggagacgag gggcgtgaag gtggagatcg ctccgtgcga cgaagaaggg tttctgcacc
     6781 ccgatatctt cgctgaggcg ctctcccgaa cccgccccga tctcgtgatc ctctcccacg
     6841 ccagcaacgt ctgcggcacc gtgcaggacc tgtcggcgct cgccccgctc tgccgcgacg
     6901 cgggcgttcc gttcgccgtc gacagcgccc agacgggcgg gatactgccg atcagcctgc
     6961 gggagctggg gcttgccgcg ctctgcttca ccgggcacaa ggggcttctc gggccgcagg
     7021 ggatcggggg tatcgtctgg aatccggaat ttgcacagaa ggtcgactgg ttcgtcgagg
     7081 gcggcacggg gagcttctcg cacctggagt tccagccgga cgcgatgccc gacaagttcg
     7141 aggccgggac gccgaatctt cccggaatcg ccggactgct cgcggcgctc tcgtggctgg
     7201 gagaaaccgg aataaacgcg gtgcgcgccc gcgaggaggc gcttggaacg cgccttctga
     7261 acggcctcct cacactcccc ggcgtgcgtc tctccgggcg gcggaccatg gaggggcgcc
     7321 tccccgtctt cgcggtgaac ttcgaggggc gcgacaacgg tctggtcgcc gggcgtctct
     7381 ccgaggagtt cggtatcgag acgcgccccg gcctccactg cgccccgctc gcccataaga
     7441 ccctcgggac cttccccggc ggcgccctcc ggctcagtcc gggctacttc tcgacggagg
     7501 aggatgtgga cgcgacgctc tcggcgcttc gtacgatcct cgattcgtga tcttttccgc
     7561 ggacacgacg gagggggtat ggtcttttcc ggcgaattag ggtatcttgt ccctgagctg
     7621 cgcgccccca acgggcgggc ggcggattgt tgttctcttt catgggccga aacgcgctcc
     7681 gggagggaag aaagccgatc atcagggccc ccgaggggcc gccggaggga tttttagaga
     7741 tgaagatagt tgtggtgggc ggaggagaag tcgggtacaa cgtggcgaac acgctttccg
     7801 aagaggagca cgacgtcacg gtcgtcgaag aggacgagga gcgggctgaa aaggtcgaca
     7861 acgaactcga cgtcatcact gtgcgcggaa gcggcgccag gccgcaggtg ctggaggagg
     7921 cgggaatctt ccccgggtgc gatgtggaca tcctcgtcgc ctgcaccgac cgcgacgagg
     7981 taaatatcct cgcctgctgg atcgccaagc gcgccggcgt aaagcgcgtg atctcccgcg
     8041 cgcgggcgct cgaattcacc gacagcccca cctgggccaa ggacctgggg atcgacgtga
     8101 tgaactcgcc ggagcgctcc gtagcccgcg aaatcctgga gctgctcttc gtcagctcgg
     8161 cggtgcacac cgcggagctg ctcgacggca ggtcggggat ctacgcgctc cgcgtgtcgc
     8221 cgaactccgc gctggtcggg ctcgcgctga aggacctgcg gacgctctac cccaacttca
     8281 tcgccatcat cgtgtacatc gagcgggacg ggacggggta cgttccgtac ggcgagatgg
     8341 tcatcgaggc gaacgacctc tgctacatcg tcacctcccg cgagcaggcg tggaagctgg
     8401 aggagatcta ccagatgaag aaaagccgcc cgctgaagcg cgtgctgatc atcggcggcg
     8461 gcaagctggg gtttcaggtc gcgtggaagc tggagagcca gttccgcaac ctggacatcc
     8521 gcctgatcga ccacaaccgg gagaagtgcg agcggctggc gtccgaactc aagcgaacgc
     8581 ttgtcctctg cggcgacggc gccgacgaga gcctgctccg gcaggagggg atcgaggggg
     8641 cggacggcct tgtctgcgcc acggagagcg acgaggcgaa tctgctcttc gcggtcatcg
     8701 gcaaggcgct cggcgcccgg aagaccatcg cggtggtcag gcgcaagatc tacatgaagc
     8761 tggacaacta catgtccgtc gacgccatcg tcaacccgaa cagcgcgctg gcctcggtca
     8821 ttctgcgcca cgcgcgctac aagtcgggag cgggggtgct ctccatcatc gagaagatcg
     8881 acgccgagat gctcgaactg acggtcccgg cggcgagccc cgccgtgggg cgcaccatca
     8941 tgagccttgg catgccgaag ggaatgctcg tcgcgctcat cgtccgcaag ggggacgtct
     9001 tcgtgccggt aggggccaca gtggtccagg ccgatgacaa ggtgattatc ttcgctacct
     9061 ccgacctgat gcccgaggcg atggactacc tgggagtgca gtagccatgc atcttcggct
     9121 cgtctcaaag gtactctcgc tgctttcggc gatcgtctcg ctctccatgc tctggccgct
     9181 cgtctggtcg ctgtgggacg gaacgcccga cgtgcgctcg ttcctgctct cgatcgcgtt
     9241 tggcctggcg cttgctcttg ttctctacgc cttcggaaag caggccgact acgacgaact
     9301 cggcatcaag gacggtttcg tggcggtctc gctggcctgg gtcatcgcct cggtcgtcgg
     9361 ggcgatgccc tactggctgc acggcacggt tcccaccttt acggacgcct tcttcgaggc
     9421 aatgtcgggc ttcaccacca ccggagcttc ggtcctctcg gacatcgagg ccaatccgcg
     9481 cggcatcctc ttctggcgcg acctcaccca ctggctcggc ggcatgggga tcatcgtcct
     9541 cagtctggcg atccttccct tcatcggcgt cggcgggatg cagctctaca aggcggaggt
     9601 tcccggcccg atccccgaga agatgacccc gagaatccag cagaccgcgc tctatctgtg
     9661 gggggtctac gtgctgctct cggcgctcca gacggcgctc ctgatgctcg gagggatgaa
     9721 cctcttcgag tcgctgacgc acaccttcgg cacgatggcg acgggcggct tctcgccgct
     9781 gaacaagagc atcggccagt acaactccgc gtacttcgac tgggtgatca ccatcttcat
     9841 gttcctcgcg ggggtgaact tcgtcctcca ctaccgtttt ctgctgggtc gctggggggc
     9901 gttctggagg gacgaggagt tccggttcta tactgtcctg accgttttct gcacgctgac
     9961 ggtgacgttc gtgttgcgtc tgcacggtac atacgactct tttgtggatg ccctgcgctt
    10021 cggcgccttc caggtggtga gcatcatcac cacgacgggg tacgtcactg ccgactacga
    10081 gctgtggccg acctatacgc agttcatcct gctgctgctg atgtttgtcg gcgcatgcgc
    10141 gggctccacc ggcggcggca taaagaatct ccggcgtatg gtgcttgcgc ggcacgtccg
    10201 ggctgaactc tcctccattc ttcacccgaa ggccatcgtg cacgtccgtg tcggggggaa
    10261 gcctgtgggg cgcgagatca tcgcttcggt aacctctttc tttatcctct atatcgcgat
    10321 ttttaccgcc ggaactcttt ttatgaccat cctcgacctc gatctgatca ccgccatgtc
    10381 gagcgtcgcg gcgacgctcg ggaacatcgg ccccggcctc ggcgacgtcg gtcccatgca
    10441 gaactacgcc gccattccgg actcgggaaa gtgggtgctc tcgctctgca tgatgatggg
    10501 gcggctcgag ctgtacaccg tcgtcctcct gctgctcccg gagacgtgga agcggtaggg
    10561 ggccgtttct tcgccgcgct gtgatagaat cggaagaaga aagaggctgc atcaaaacga
    10621 cgaatcggag gggattttcg tgaagctcat cgacatgaac cgtctggaga acgagcagat
    10681 tcttcccgga ctgcgcgcgc gtttcgtcaa cgcggagaac gtgtccttcg cgcagtggac
    10741 catggaggcg ggcacgttct ttccgctgca cgaccatgcg cacgagcaga tactgaacgt
    10801 gatctcggga aaggtggaga tgaacgtcgc cggcgagaag gtgatcctcg acgaagggtg
    10861 cgccctcgtt ctcgctccgg gggagcccca tgcgatgaag gtgctgacgg acaccttcat
    10921 ggtcggcgtc taccacccgc gccgcgacgg gtataaaaaa tgaccgcagg cggccttcgg
    10981 gccgtctttc cagtatgcga agaggggcgg agggggcatt ttcggcgttc ttcggccctt
    11041 cgttccgcat cgaatggagc gcaccgctca tggaggttcc gaaatgacgg atgaaacgta
    11101 ccgggcgatg ccccttccct tctcctcatc ttccggctgt acggaggagg ccctccggga
    11161 gagcgaggag aacttccgca cgttcttcga ggccatcgac gagctggcga gcgtcatctc
    11221 gctcgacggc aacatcctgt tcaccaacgg cgcattccgg cgaaagacgg gctacgccga
    11281 ggatgaaatt gtagggatga gcgcgctgga tctggagctc cccgagaacc gggagaaggg
    11341 cgccgaggtc ttcgcggcca tgctgcgcgg ggagctgaag agctgttcta tgcccatgcg
    11401 ggcgaaggac ggctcgcacg tcctcgtcga aacgcggctc tggttcggcc ggtggaacgg
    11461 cgcgccgtgc gtctacaccc tcagcaagga cgtcggaccg gagcaggagg cgcagcagcg
    11521 cttcgaggcc gtcttccgga acaaccccgc catgatggtg ctcgcctcgc tgcccgacca
    11581 gcggtgcgtt gacgcgaaca ccacgttttt gcggacgctc ggctacgaga aggaggacgt
    11641 cgtcggcaaa caggccaggg agatcggtct tttcatcgac ccggaacagc agcgcgagat
    11701 cggcgagatg atgcgaaacg acggcaggat caccgactac gaggtgcacg cgaagaaccg
    11761 gaacggcgac gtcttccacg gcctcatgtc cgccgaccgc atttctatcc acggacgccc
    11821 ctatcttctg acggtgacgg tggacatcac agtccgcaag cgcgcggagg aggcgctcct
    11881 ggtagccaag gagcaggccg agtggcttgg gcggcaggcg gaggcggcga acagggcgaa
    11941 gagcgccttc atcgcgaaca ccagccacga gatccgcacc ccgctcaacg gcgtcgtcgg
    12001 cttcctcgac ctcctggcgc gaactcgtct cgacgacgtg cagcgggagt acgtcgaggg
    12061 gatacgcgcg tcggcgggct ctctcctcga cgtgatcaac gacgtgctcg acatctcgaa
    12121 gatcgaggcc gacaaggtcg agctgcggcc ggcgccgacg gatatccggg agcttgcggg
    12181 gcaggcggcg gatacagtcc tcggcacggc gcggacgaag gggctgcatc ttacggtgaa
    12241 cgtcgccccg gaggtcccgg gggcgttctt cctcgaccag tcgcgcctga agcaggtgct
    12301 gatcaatctg ctgggcaacg ccgtcaagtt cactcatgag gggggcgtgt cgttctcgat
    12361 ccgctgcgag gaggctgaag aggaccgatg ccttctccgc ttctccgtcg aggataccgg
    12421 gatcggcatt cgtccggaat ccatggagcg cctcttcgag cccttcttcc aggccgacgt
    12481 ctcgaacacg cgaaagtacg gcggcaccgg actcggcctc tccatctgca gcgcgctcct
    12541 tcagatgatg gggagcgaac tgaaggtcga gagcgtcccc ggcgagggga gccgcttttt
    12601 cttcgacctg cgggcggagc gcgtcccggt ttcggagacg acagaggaag agagagcgcg
    12661 gatcgttccg gaggagccct tcatgccggc gacgagaccg accatactga tcatagagga
    12721 cgaggagctg aaccggcggc tgttgcgcct gctgctctcc aagctgctcg atgcggacat
    12781 cgtcgaggcg gagggggggg aagagggtct cgaacgcttc cgcgagcgcg ctcccgacct
    12841 gatcttcctc gacctccaga tgcccggacg tgacggccac tggacggcgc ggacgctgcg
    12901 ggagctggag ggggcggggg tgcatacgcc gatcgtcgcc ttcaccgccg acgtgcagcc
    12961 cgcgacgaga gaggcctgtt tcgcgagcgg catggacgac tacatctgca aacccgccga
    13021 cctctacacg ctccgcgagg tgctggagcg gcatctgaag acgaaagagt agcgggacgt
    13081 ccggcgcccc gccggagacc gccgcgacga cgcacacaac gaaaggaaga agcgcgcatg
    13141 atcgaactga agcacctgac gacggaacag cgcaacgaaa agacgctcga cctggacagc
    13201 atgcccctcg acgaggccct ccgggtgatg aacgcggagg acgcgaaagt cgcccccgca
    13261 gtgggggaac agctccccgc cattgcgaag gcggtggagg ctgtggtccg ctctttcgag
    13321 agcggggggc gactgctcta cctgggcgcg gggaccagcg gacgcctggg aatcctcgac
    13381 gcgacggagt gcgttcccac cttcggcacg ccgccggaga tggtcgtggg acgcatcgcg
    13441 ggcggcatgg gagcgctggt gcgctccgtg gagggcgcgg aggacagcct gaccatgggt
    13501 gtggacgacc tgaaggaact caaggccgac tccagggaca ccgtggtggg gatagcggcc
    13561 agcggacgca cgccgtacgt gatcgccgcg ctggagtacg cgaagagcgt cggctgcgcg
    13621 acggtgggcg tcacatgcaa taaaaactcc gcggtgggcg ctgcggcgga catcgccatc
    13681 gagatcgacg tcggccccga ggtgctcacc ggctccacgc ggctgaaggc ggggacggcg
    13741 cagaagctgg cgctcaacat gatctccacg atggcgatgg tgggcatagg caaggtgtac
    13801 aagaacctga tggtcgacgt gcagcggacg aacgaaaagc tccggacgcg ggccgtcaac
    13861 atcgtcgccg acgctgccga ggtcgataag gaaaccgccc g
//
LOCUS       DAOPKU010000009        13653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_4720_length_13653_cov_4.462642, whole genome shotgun sequence.
ACCESSION   DAOPKU010000009 DAOPKU010000000
VERSION     DAOPKU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13653
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4720_length_13653_cov_4.462642"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK849_00595"
     CDS             <1..399
                     /locus_tag="PK849_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE64647.1"
                     /translation="KYAQQLTKELDIRCTGPSQLTRRLSGGNQQKVCIARAITLAPNL
                     LFVSEPTRGIDIGAKKLILDHLVKLNRENGLTIIMTSSELAELRSICDRIAIVYQGKL
                     EGILLPADSDRDFGLMMAGEYHKFHEKEAV"
     gene            401..1441
                     /locus_tag="PK849_00600"
     CDS             401..1441
                     /locus_tag="PK849_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64648.1"
                     /translation="MGNSLLNRIGIPRLIISLFLAFLVASAFSYGLPMGQIFGAMLTR
                     FGMNALLVLAMIPTIQAGAGPNFGLPFGIICGLIGATLAIELDLRDFTALFFAIGVSV
                     PLGAFAGWLYGLLLNRVKGQEMTVGTYMGFSIVSLMCIFWLMAPYTSPEMVWPYGGDG
                     LRVTVVLDGRMEQLLDKLWSFTIGTVRIPTGLLVVTAICCVLLWIFMKTRTGLAMSIV
                     GSNPRFAEASGLNVNKYRLIASVISCAMGAAGIVIYSQSYGFIQLYQAPLYMALYSVS
                     AILIGGASLQRATITQALIGTFLFNALLVIALPVANVAMDSDISEIMRVIISNGIILY
                     ALTRKEVGGDAR"
     gene            1438..2511
                     /locus_tag="PK849_00605"
     CDS             1438..2511
                     /locus_tag="PK849_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64649.1"
                     /translation="MKLIQKNIVPIVFIILCTIGFQYSGLSWIFFANDLMTRLARNSF
                     LVISLIIPVLAGMGLNFGIVLGAMAGQFAAFITVVHNLTGIGGFFAACVMAIPFAILF
                     GWLTGILFNKAKGKEMITGLILGFFANGIYQLVCLIFIGWLIPISNKALLLPSGVGFV
                     NTLDLKIWQYAIDKFYVFRARGIDIPVLKYLNNINIPVLTLTIVVLLCIAVVLLFRTK
                     LGQDFRAVGQNRHIAEIAGIKVDRVRIIAVIFSTVLAAWGQLIFLQNIGNVQVYGSHV
                     QVGTFAVAALLIGGATVSKATIGQALLGTVLFHALFIVSPLAGKNLMGSAQVGEYFRV
                     FIAYGVIGIALALHAWQRLVKKQ"
     gene            2726..4453
                     /locus_tag="PK849_00610"
     CDS             2726..4453
                     /locus_tag="PK849_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPE64650.1"
                     /translation="MSENGFACYSLREELLLALNKKGFSIPTPVQEKVLSMDRFDTDL
                     IVRAKTGSGKTLAFLLPLLQEMELPASAPLILVLSPTRELAQQTAREAEWLSRYLDIS
                     VATLVGGLDMTTQIRSLKEGAVVVVGTPGRTLDHVNRGSLKTEKIHTVILDEGDHMLD
                     MGFRDELEGILDALPHRNRTWLFSATMPREVKELSRKYLSSPVSISLVEDGDQHEDIV
                     HKAYLVPFRHKMEGLVNVLLWERPKRGLIFCHTRLETMNVSQRLNEEGFNSGSLHGEM
                     TQRERNAVLSAFKNGHLPLLVATNVAARGLDVEGVTHVIQIGLPDDRDTFVHRSGRTG
                     RAGHEGMDILLLSPQEAEKFKYMIGSAKVNIEWNNVPDVQAIGKVQREIAEEKLLTLK
                     EASENEDYLQWAEDLLTRVNPKNLVARLLFSLTSKRSAGYNLAADLERELRRKSRGPV
                     EKDRTPGNRSLKGGRPKGTMLRLSKGLADGWDVGRVLHSVCTSLNVDRSEVGAIRMKD
                     DHVLVELLPVALSRFEEDSRGLERCGLIEEGKGRDMLFQAYSTSRPSRDRAPSPRPRR
                     QSKTDSRNR"
     gene            complement(4514..4714)
                     /locus_tag="PK849_00615"
     CDS             complement(4514..4714)
                     /locus_tag="PK849_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPE64651.1"
                     /translation="MSQGTVKWFNDSKGYGFITTDEGKDVFVHFSAITGDGFKTLAEG
                     QKVSFEVVDGEKGPQAANVHKA"
     gene            complement(4948..6267)
                     /locus_tag="PK849_00620"
     CDS             complement(4948..6267)
                     /locus_tag="PK849_00620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE64652.1"
                     /translation="MHEKSALIREDERKSMEKRALAYRWVVWGVMVFAFMVVFFHRLA
                     AGVVKEDVTSAFSLSATAFGAMSSMYFYAYISMQIPVGLLADSLGARITVSVGMFAAG
                     AGSILFGFAPSYFWLLAGRFLVGLGVATVFVCIMKIQSQWFRDREFATISGATTFIGN
                     AGGIFSQGPLAFLLTIVSWRSSFIAIGIATLGISLLCYRLIRNRPQDMGFPPLNEREM
                     LREEMEAEPFSVLKGLKTVLSVRGVRIATLFYFFNQAGLFAFLGTWGTPWLVHVFEMS
                     IATASSLMVMTVIGIMIGGISTGWISDKIGRRRLPMIIAASVHSVLWGCMIFPWQQPL
                     GVGALRCIFLLLGFTNTAFVLSWSVAKELTSKKYTGLAISVLNAAGFFAIAVTTSIMG
                     MVIDYYSFLPLEAAYRRAFSLGLICSVFSLLFALLIPETGFMKRKEK"
     gene            6441..8399
                     /locus_tag="PK849_00625"
     CDS             6441..8399
                     /locus_tag="PK849_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE64653.1"
                     /translation="MIHLINITLRFGERVLFKNISWSIPKGSRVGLVGSNGAGKTTLL
                     RILSGEMESDSGEVSLAKDQSIGYLPQDLIEIPDVPVLDFLRERTGLADIERRLASCT
                     RNLTEAVPGSEEGNRLLTLHDELMKQYEMRDGFAFEPRARKILKGLGFTDEDAFSTCG
                     NFSGGWKMRLHLASLLLISPDILLLDEPTNHLDTESMEWLEGWLSVFPGTIIAVSHDR
                     RFLDTMCTSVAELSLGSLSLYQGNFSAYVEERERRIDDLRKAQKQQKEEIAHIEEFIE
                     RFRYKASKASSVQSRIKRLEKIELVEIEEETKRVHFRFPPCTRSGLESIVLKDVEKRY
                     GDKIVFHDASLSIQRGEKIALVGVNGAGKSTLSRILGGTEQPSSGSVRFGHNVKLAFF
                     SQESSQNLDYSRTVWDTISSRNAAWSEREKRSLLGAFLFGGDDIYKPVSVLSGGEKSR
                     LALLKLLLEEANVLILDEPTNHLDMQTKDLFQRALLEYDGTLVIVSHDRFFLDNLVDK
                     VIEITSGSLIVYPGNYSWFIEKRRERLASEASLLSFGNANASSEKSSQDGIRNLKRME
                     AEKRNALYRRKKRVLDTMEPLEARIEHLERDQAQRDAQLCDPSFLADSVKVSAILIER
                     NEAAREIADLLAQWEELAEEVDRIERTG"
     gene            8651..9286
                     /locus_tag="PK849_00630"
     CDS             8651..9286
                     /locus_tag="PK849_00630"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HPE64654.1"
                     /translation="MILENEPNDEGLIRELYLDVTGCDIGGVRIDSLKLRAVGVTLSP
                     PSQWEQEGGLDAKEVLNVHAFASITEKDLNENLLQKQFGDDDEWHNLQVRMTPDGIYA
                     RGNYHAQVLFTLNILIEIFSKFKIVDMQQVWLDDYTLRVNRVDVPDFITEKAVSQIQP
                     LLDLSKFVFPLRLSSITFKEGSMTIASRLLPEPFEGIVYTYEKNKKTEEKK"
     gene            complement(9339..10235)
                     /locus_tag="PK849_00635"
     CDS             complement(9339..10235)
                     /locus_tag="PK849_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE64655.1"
                     /translation="MDFHLVDLPLSRPGFRRFVSAWLLRDTARDRTYIVDPGPPSTWP
                     LLRDAVHSKGNGRVDAVLVTHVHIDHAGASGLVVREFGALVAAPEKGVPHLLDPSALW
                     KGSRQTLGETALLYGEPPPVPAEKILSIDSLPKGFFAVDTPGHAAHHQSFLFDDDGVL
                     TLFAGEAAGVYLEGEEPFPYLRPATPPRFFPDVTLASLDRLLERGCSRICYGHYGAAE
                     GAREMLTFAKEQLLFWKDVILGLLRNTADPRNEEFFLEELLERDPFLAAWRRMDHDIR
                     ERERMFLCNSIRGYLGAFSREK"
     gene            complement(10245..10931)
                     /locus_tag="PK849_00640"
     CDS             complement(10245..10931)
                     /locus_tag="PK849_00640"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPE64656.1"
                     /translation="MFYGKRKVPEWAKQVAVLIVLLVLTVSAWQWYRILEHRTEQEFS
                     APVPAPSPAPTPVPAPSPEPRPDLEPGPLPTAEPPAVIQPPVAPHEARIDEAWLHVVK
                     GQYRMYFYRGETVERVYQIAIGANGGQKLRVGDHRTPVGSFSVQQIQDSRKWTYDFGD
                     GKGAIRGAYGPWFIRLKTPGWKGIGIHGTHDPDSIGRMITQGCVRLRNEDLEELKDKV
                     FIGMKVIISE"
     gene            11125..12300
                     /locus_tag="PK849_00645"
     CDS             11125..12300
                     /locus_tag="PK849_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HPE64657.1"
                     /translation="MENEFQFDLERYMSQANFNRIKDFAQDKETPFLIVDLKKVEEKY
                     DELLDSLPFAKIYYAVKANPLEQVLRMLVQKGSCFDIASIYELDHILALGASPDRISY
                     GNTIKKARNIAYAYSKGVRLFATDSESDLRKLAKNAPGARVFFRILTDGSGADWPLSR
                     KFGAHPDTIFKLILLAAKLGLEPYGISFHVGSQQRDIGQWDNAISACKYLFDCAEAEG
                     ITLKMLNLGGGFPATYISPTHETSVYAAEIIRFLKEDFGENLPEIILEPGRFMAGDAG
                     VLVTEVVLISKKSEANQYSWVYLDAGKFGGLIETLDESIKYPIYSEKKGPVQETILAG
                     PTCDSMDILYENEKYALPASLEEGDRLYFFTTGAYTQTYSSVCFNGFPPLKAYVLEK"
     gene            12404..13249
                     /gene="speE"
                     /locus_tag="PK849_00650"
     CDS             12404..13249
                     /gene="speE"
                     /locus_tag="PK849_00650"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPE64658.1"
                     /translation="MEIRQKRTNELWFTEEQSVNMKLSLRVSDVLLNITSPYQDILLI
                     QTAEYGRMLVLDGAIQITERDEFCYSEMMAHVALCSHPSPKRVLIVGGGDGGVLREVL
                     RHKCVEKATLVDIDEEVINASKKFLPTINVALEDPRADVRPMDAIEFIASSDDEFDVA
                     IVDSTDPVEFAAGLFRSPFYRGIFKALKKDGMVVAQTESPFTDASVVKGAFKEMSAVF
                     PIVRMCWGAMPTYPSGMWTYTIGSKTVDPADIRRPSPDGTKYYTEDIHKAAFVLPPFV
                     KDLLK"
     gene            13308..>13653
                     /locus_tag="PK849_00655"
     CDS             13308..>13653
                     /locus_tag="PK849_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPE64659.1"
                     /translation="MRVLVALGGNAILQRGQKGNASEQRANVQKTVTQIVRMIHAGHE
                     VVVTHGNGPQVGAILIQNELGSASVPAMPMDICGAESQGLIGYMFCQSIQNTLLADGI
                     EGHAPACLVTRVE"
BASE COUNT         3377 a         3656 c         3417 g         3203 t
ORIGIN      
        1 aaatacgctc agcaactgac caaagagctc gatatccgct gcacaggtcc gtcgcagctg
       61 acacggcgcc tctctggagg caatcagcaa aaagtctgca tcgcccgcgc catcacgctt
      121 gcgccgaatc ttctcttcgt ctccgaacct acgcggggta tcgacatcgg cgccaagaag
      181 ctcatcctcg atcatcttgt caaactgaac agagagaacg ggcttactat aatcatgacc
      241 tcttctgagt tggcggagct tcgctccatc tgcgaccgga tcgcaatcgt ctaccagggg
      301 aaactggaag gcatactgct tcccgcggac agcgaccgcg attttgggct tatgatggca
      361 ggagagtatc ataagttcca tgaaaaggag gctgtctgat atggggaaca gtcttctgaa
      421 ccgcatcggg attccacgcc tcatcatctc ccttttcctc gcatttcttg tcgcttcggc
      481 tttcagttac ggccttccca tgggacagat cttcggagct atgcttacgc gtttcgggat
      541 gaacgcactt ttagtgctcg caatgattcc gaccattcag gccggagctg ggccgaattt
      601 cggccttccg ttcggtatca tctgcggact tatcggcgcc acgctcgcta tcgaactcga
      661 tctgcgagac ttcacagcgc tcttcttcgc gatcggggtc tccgtacctt tgggggcatt
      721 cgcgggctgg ctttacggct tgctgctcaa cagggtcaaa ggacaggaga tgacagtcgg
      781 cacctatatg ggattctcta ttgtgtcgct gatgtgcatc ttctggctca tggctcccta
      841 tacaagccct gaaatggtct ggccctatgg tggagatggt ctccgggtca ccgttgtgct
      901 cgatggcagg atggagcaac tcctggacaa gctgtggagc ttcacaatag gcacagtacg
      961 gattcctacc ggcttgcttg ttgtgacggc gatctgctgc gttcttctct ggatctttat
     1021 gaaaacacgt acgggtcttg ccatgtccat cgtcggctcc aaccctcgct tcgcggaagc
     1081 gtcggggctg aacgtcaata aataccgtct catcgcttcg gtcatttcgt gtgcaatggg
     1141 cgccgccggt attgtcatct actcgcagag ctacggtttc atccagctct accaggcccc
     1201 cctctacatg gcgctctatt cggtatccgc cattctcatc ggcggcgctt cgctgcagcg
     1261 ggcgacgatc acgcaagcgc tcataggcac attcctcttc aatgcgctcc tggtcatcgc
     1321 gctcccggta gccaatgtag ccatggacag cgacatctcg gagatcatgc gggtcatcat
     1381 aagcaacggc atcattttgt acgcgctcac ccgcaaggaa gttggaggtg acgccagatg
     1441 aagctcatac agaaaaacat cgttccgata gtgttcatta tcctctgcac cataggattc
     1501 cagtactcgg ggctctcttg gatattcttc gccaacgacc tcatgacgcg gctcgcccgg
     1561 aactccttcc tggtgatctc cttgatcatt cccgtccttg cgggcatggg attgaatttc
     1621 ggaatcgtcc tcggagcgat ggccggacag tttgcggcgt tcatcacggt cgtgcataac
     1681 ctcacgggga tcggcggttt cttcgcggcg tgcgtgatgg cgattccatt cgccattctc
     1741 ttcggctggc tgacgggcat cctcttcaac aaggccaaag ggaaagagat gatcaccgga
     1801 ctgattcttg gcttctttgc caacggtatc taccagctgg tctgcctgat ctttatcgga
     1861 tggctgattc ccatctcgaa caaggcgctt ctcctgcctt ccggtgtcgg ttttgtgaac
     1921 acgctggacc tgaagatatg gcagtacgcg atcgacaagt tctacgtctt ccgcgcccgc
     1981 ggcatagaca taccggtgct caaatatctg aacaacatca acattccggt attgaccctg
     2041 acgatagttg ttctcctgtg catcgccgtc gtccttcttt tcaggacaaa gcttgggcag
     2101 gacttccggg ctgtgggaca gaacaggcat atagcggaaa tcgcgggaat caaggttgac
     2161 agggtacgta ttattgccgt catcttttcc actgttctgg ctgcatgggg acagctcatc
     2221 ttcctccaga acatcggaaa cgtgcaggtt tacggttctc atgtgcaggt cgggacattt
     2281 gcggttgcgg cgcttctcat cggaggcgca accgtctcga aagccactat aggtcaggcg
     2341 cttcttggaa ccgtactctt tcacgccctt ttcatcgtct ccccacttgc cgggaaaaat
     2401 cttatgggaa gcgcacaggt aggcgaatac ttccgtgttt tcattgccta cggcgtcatt
     2461 ggcatcgctc tggcgcttca cgcctggcaa cgattggtaa aaaaacagta agattctcgt
     2521 cacacagatt cgcgcctcca tattgacata ttctgaaatt catgtataat ttcagtttgt
     2581 caatggaggg aagaatttga gtggaccccc ggaacggggt ccactctttt ttggggaacg
     2641 cgggccgaac aaagaacggc aagtactatt tgatgcatta ttccccagga tttgccctcc
     2701 taatttgagg aggaataata tactcatgag tgaaaacggt ttcgcgtgct attctctgcg
     2761 ggaagaactt cttctcgcgc tcaacaagaa aggcttttca atccccacac cggtccagga
     2821 aaaagtgctt tcgatggacc gtttcgatac cgacctgatc gtccgggcaa agacgggatc
     2881 gggcaaaacg ctggcgttcc tgctccctct gctccaagaa atggagcttc ccgcatcagc
     2941 ccctctcatc ctggtgcttt cacccacccg tgagcttgca cagcaaacag ctcgtgaggc
     3001 ggagtggctt tcacgttacc tcgatatttc cgtcgccact ctcgtcggcg ggctggatat
     3061 gacgactcaa atccgttcgc tcaaagaagg cgcagtcgtc gtcgtcggga ctcccggaag
     3121 aacgcttgat catgtcaacc gcggttcgct gaagacggag aaaatccaca ctgtcatcct
     3181 ggacgagggc gatcacatgc tcgacatggg attccgcgac gaacttgaag gcattctgga
     3241 cgcgctcccc catcgtaacc ggacgtggct tttctccgct actatgccga gggaggtcaa
     3301 ggagctctcc agaaagtacc tctccagccc cgtttccatc tctttagttg aagacggcga
     3361 tcagcacgag gatattgtcc acaaggccta cctggttcca ttccggcata agatggaggg
     3421 attggtgaac gttcttcttt gggagcgccc gaagcgggga ctgatcttct gccatacccg
     3481 tcttgaaacg atgaacgtct ctcagcgtct taacgaggag ggcttcaatt ccggctcgct
     3541 ccatggagag atgacccaga gagagcggaa cgccgtcctt tcagccttca agaacggcca
     3601 tcttcccctt ctggtcgcaa caaatgtagc ggcgcgcggc ctggacgtcg agggagtgac
     3661 ccacgtcata cagatcggcc ttcccgacga cagggatacc ttcgtccacc gcagcggacg
     3721 tacaggcaga gcaggacacg aaggtatgga tatccttctt ctctctccgc aggaagcgga
     3781 aaagttcaag tacatgatcg gctccgccaa ggtgaacatc gaatggaaca acgtcccaga
     3841 cgtgcaggct atcggcaagg tacagcgaga gatagcggaa gaaaaactgc ttacgctgaa
     3901 agaagccagt gaaaatgaag actaccttca gtgggccgaa gacctgctga caagggtgaa
     3961 tcctaaaaac ctcgtcgccc gtcttctctt ttcactgaca tccaagcgct ctgctggata
     4021 caatctcgcc gccgatcttg aaagagagct tcgaagaaaa agccgcggcc ccgttgaaaa
     4081 agaccgcact cccggcaatc gttctttaaa agggggccgc cccaagggaa ctatgcttcg
     4141 cttgagcaag ggacttgccg acggatggga tgtgggacgg gtgctgcatt ccgtgtgtac
     4201 gtcgcttaac gttgaccgct cggaagtcgg cgctatccga atgaaagacg accatgtcct
     4261 ggtggaacta cttccagtgg ctctttcccg ttttgaagag gattctcgcg gccttgaacg
     4321 ttgcggactt atcgaagagg gaaaagggag agatatgctc ttccaggcgt actcgacttc
     4381 gagaccatcg agagaccgcg ccccttctcc cagacccaga agacagagta aaacagattc
     4441 cagaaataga taagtataaa gccggcctcg tttgaggccg gctttatcaa atattattat
     4501 aatcggggaa taattacgcc ttatgaacgt tagcggcctg agggcccttt tcgccgtcca
     4561 ctacctcaaa acttaccttc tggccctcgg caagcgtctt aaaaccatcg cctgtgattg
     4621 cgctgaaatg aacgaaaaca tctttgccct cgtccgtcgt aatgaaacca taccccttgc
     4681 tatcgttaaa ccatttcaca gttccctgac tcatccaaag acagcctcct aaaactttat
     4741 taaattctgc ggtccatatc ctgatgccgg actgcaaatc aacaatatag gattttaggc
     4801 cttatgtcaa gcttttttta aaacttttcc tttttacgtt cgtatcgatt ccttttttac
     4861 ccccctcgag tctttggcat gattaaaatg aaaaggcttt tcgtgagatg aaattaaact
     4921 aaatttcata tagagtccac cgcgcgttta tttctctttt cttttcataa accctgtttc
     4981 tggaataaga agagcgaaaa gaagagagaa aaccgaacaa atcaacccca acgagaacgc
     5041 ccttcgatat gcggcttcca gggggaggaa cgaatagtag tcgatcacca ttcccataat
     5101 ggatgtcgtc actgcaatgg cgaaaaaacc cgccgcattg aggactgaaa tcgccaatcc
     5161 ggtgtacttt tttgaagtca actcttttgc cacggaccag gaaagcacga atgcagtatt
     5221 ggtgaatccg aggaggagaa aaatacaccg cagcgccccg acaccgagcg gttgttgcca
     5281 tggaaagatc atgcaccccc aaagcactga atgcactgat gcggcgataa tcatggggag
     5341 ccgtcttctc ccgatcttgt ccgagatcca tccggtagag ataccgccta tcatgatacc
     5401 gatgactgtc atcaccataa gggatgaagc cgtcgctatc gacatttcaa agacatgcac
     5461 aagccatgga gttccccacg tcccgagaaa agcgaacaaa cctgcctggt tgaagaagta
     5521 aaagagcgtc gcgatacgta cccccctcac agagagcact gttttcaatc ccttgagaac
     5581 ggagaacggc tctgcttcca tttcttcacg gagcatctcg cgttcgttca acggcgggaa
     5641 gcccatatcc tgcggacgat ttcgaatcaa cctgtaacat agaagagaaa tacccaaagt
     5701 cgctattccg atcgctataa aacttgatcg ccaggatact attgtgagaa ggaaagcaag
     5761 gggaccttgc gaaaagattc cgccggcgtt accgataaac gttgttgcac ccgagattgt
     5821 ggcgaattct ctatcccgga accactggga ctgtattttc attatgcata caaaaactgt
     5881 cgcgacgcca agccctacca agaaacggcc ggcaagaagc caaaaatacg acggagcgaa
     5941 gccgaaaagt atcgatcccg ctccggcggc gaacatcccc accgaaacgg ttatcctggc
     6001 tcccagggag tccgcgagaa gtccgaccgg tatttgcatg gaaatatatg cgtaaaagta
     6061 catcgaagac atggcgccga acgctgtcgc gcttaatgaa aacgcactcg ttacatcttc
     6121 cttgacgact ccggcggcta accgatgaaa aaaaactacc ataaatgcaa aaaccattac
     6181 tccccatact acccatcggt aggcaagcgc ccgcttctcc atagattttc gctcatcctc
     6241 acggatgagc gcactctttt cgtgcatgtg aacaattcct ttcttcacgc cccttcgcag
     6301 gtcggcgtat tctacgcatt cattttacaa tgctgtagaa gataatgata gcatcataca
     6361 atttttccaa acctctttta tcaatacgcc gtacccctta taatgaaccc tgtacacgag
     6421 gaacaaggag gaacttttcg gtgatccatc taataaatat cacgctccgt ttcggagaac
     6481 gcgtactttt taaaaatatt tcctggtcta tacccaaagg ttccagagtc ggccttgtgg
     6541 gaagcaacgg agccggaaaa accacactgc tccgcatact ctccggagag atggaatccg
     6601 actcgggtga agtatctcta gcgaaagatc agagcattgg ctatcttccg caggacctca
     6661 tcgaaattcc ggatgttcct gttcttgact ttcttcgcga gagaaccggc ctcgccgaca
     6721 tcgaaagaag gcttgcgtcg tgtacgcgga atcttacgga agctgttcca ggatccgaag
     6781 agggaaacag gcttctgacg ctccacgacg aactcatgaa gcagtatgaa atgcgagacg
     6841 gtttcgcttt cgaaccaagg gctcgaaaaa tcctcaaagg actcggtttt accgatgagg
     6901 acgctttttc tacctgcgga aatttctccg gaggatggaa gatgcgcctt cacttggcgt
     6961 ctctgcttct catctctccc gacatactcc ttcttgacga gccgacgaac caccttgata
     7021 ccgagagcat ggagtggctg gaagggtggc tttcagtgtt tccggggacg attatcgccg
     7081 tatctcacga taggcgtttc ttagatacga tgtgtacttc ggtcgcggag ctttcactgg
     7141 gatccctttc actgtatcag gggaattttt ccgcgtacgt ggaagagagg gaacgtcgca
     7201 tcgacgatct ccgcaaggcc cagaaacaac aaaaagaaga gattgcacac atcgaggaat
     7261 tcattgagcg attccgttat aaggcctcca aggcgtcttc ggtacagagc aggatcaagc
     7321 gtcttgagaa gattgagctt gtcgaaatag aagaagagac aaagcgtgtc catttccgct
     7381 ttccgccgtg tacgcgaagc ggacttgaat cgattgtcct caaggatgtc gaaaaacgat
     7441 atggcgacaa gatcgttttt catgatgctt ctctctccat tcaaagaggg gagaaaattg
     7501 ctctggtcgg ggtgaacggc gcagggaagt cgacgctctc acggatcctg ggcgggacgg
     7561 aacaaccttc ctctggctcg gttcgtttcg gccataacgt caaactcgca ttcttctcgc
     7621 aagagtcctc tcaaaatctg gactactcaa ggactgtctg ggatactatc tcttctcgga
     7681 acgcagcgtg gtcggaacgt gaaaagagga gccttctcgg ggcgttcctc ttcggaggcg
     7741 acgatattta taagcccgtt tctgttttgt ctggagggga aaaatccaga cttgctctcc
     7801 tgaagcttct tctggaggag gcgaacgtac tgatcctcga cgagcccacg aaccatctgg
     7861 atatgcagac gaaagacctc ttccagaggg cgcttctgga gtacgacggc acgttggtca
     7921 ttgtttcgca cgaccgtttc ttcctggata atttagtcga caaagtaatc gaaatcactt
     7981 ccggttcttt gatcgtctac cccgggaact actcctggtt catcgaaaaa agaagggaac
     8041 gtcttgcttc ggaagcttct cttctttcct ttggaaacgc aaacgcatca tccgagaaat
     8101 cgtctcagga cggaatacga aaccttaaac ggatggaggc tgagaaacga aacgcgctct
     8161 acaggcgcaa aaaaagggtt ctggacacaa tggaacctct tgaagcgcgt atcgaacacc
     8221 ttgaaaggga ccaggcccaa cgcgatgctc agctctgcga tccttcgttc cttgcagatt
     8281 cagtcaaggt ttcagcgatt cttatcgaac gaaatgaagc cgcccgcgag atagcagacc
     8341 ttctcgctca atgggaggaa ttggcggaag aggtggatcg tatcgagaga acggggtaaa
     8401 acgccgatat accaagagac cttcaaaaaa tgcactgcat atctcgtgag gaggacacat
     8461 gatgcgtatt tttttaattc cgtacagtcg cggcaatacc agagaaaggg cctttctcct
     8521 tctctttgtt tttagccttg cactgttcat gctatcgcag gcaaacacag cttccgctga
     8581 aacggatcaa acgcgggggg aaaaactgct cctgcatttc gtagaggagt tcaaacctga
     8641 aaaaatacac atgatcctgg aaaatgaacc taacgacgaa ggccttatcc gggaattgta
     8701 tctcgatgtc acgggctgcg acatcggcgg ggtccgtata gattctctga aactacgcgc
     8761 agtcggcgta acgctctccc ctccttctca atgggaacaa gagggcgggc tcgacgcaaa
     8821 agaagtactc aatgtccatg cgttcgcctc cattacggag aaagacttga acgaaaattt
     8881 gcttcaaaaa cagttcggcg acgatgacga atggcataac cttcaggttc ggatgacccc
     8941 ggacgggata tacgcgcgcg gtaattacca cgcccaggta ctgtttacgc tcaatatcct
     9001 catcgagata ttcagtaagt tcaagatcgt cgacatgcaa caggtgtggc ttgacgacta
     9061 tacgctccgg gtgaaccgtg tcgacgttcc ggacttcatc acggaaaaag cagtctctca
     9121 aattcaaccg ctcctcgacc tgagtaagtt cgtctttcct ttgcgcctct cttccattac
     9181 cttcaaggag gggagcatga cgatcgccag ccgtcttcta ccggaacctt tcgaaggaat
     9241 cgtctacacc tacgagaaaa acaaaaagac tgaggagaaa aagtaaacgc acgctgaata
     9301 caaagcgggg aggttccgaa cggagccctc cccgttattt atttctcccg tgaaaacgcc
     9361 ccaaggtacc ctcttatgct attgcataaa aacatacgct cccgctctcg tatatcgtga
     9421 tccatacgcc gccaggcggc aaggaaaggg tcccgttcca ggagttcttc caggaaaaac
     9481 tcttcgtttc tcggatccgc tgtgtttcga aggagcccga ggataacgtc cttccagaac
     9541 aacagctgct ctttggcgaa tgtgagcatc tctctcgcac cttccgccgc gccgtaatgt
     9601 ccgtagcaga ttcgggagca tcctcgctcc agaagccggt caagggaagc aagcgtgaca
     9661 tcggggaaaa aacggggcgg tgtcgccggc cgaagatagg ggaagggttc ttccccttca
     9721 agatagactc cggcggcttc tccggcaaag agagtcagca ccccgtcgtc atcgaaaagg
     9781 aaactctgat ggtgcgccgc atgacctgga gtatccacag cgaaaaaccc tttcggcaga
     9841 gaatctatcg aaaggatctt ctccgctgga acaggaggcg gctcaccgta gagcaaagcc
     9901 gtttcaccaa gcgtttgcct gctgcccttc cagagagctg atgggtcaag cagatgcggc
     9961 acgccttttt caggggctgc aacaagagcg ccaaattctc tgacgacaag ccccgatgct
    10021 ccggcatgat caatatgaac gtgggttacc aacactgcgt cgacccgtcc gtttcctttg
    10081 ctgtgcaccg catcacgaag cagaggccac gtggaaggcg ggccagggtc gacaatgtat
    10141 gtcctgtctc ttgcggtatc tcgtagaagc cacgctgaaa cgaaccgcct gaaaccgggt
    10201 ctcgatagcg ggaggtcaac cagatgaaaa tccatcacgc cctcctattc cgaaataata
    10261 actttcattc caatgaagac cttatctttt aattcttcga gatcctcgtt cctaaggcga
    10321 acacaccctt gagtgatcat cctcccgata gaatcaggat cgtgggtacc gtgaatgccg
    10381 attcccttcc aaccaggggt tttcagtcga atgaaccaag gcccgtacgc gcctcgaata
    10441 gctccttttc catcgccgaa atcatacgtc cattttcttg aatcctggat ctgctgtaca
    10501 gaaaaactcc cgacaggcgt acgatgatcc cccactctca gtttctgtcc gccgttcgct
    10561 ccaatggcaa tctggtatac gcgttcgaca gtctctcctc tatagaagta cattcgatac
    10621 tgccctttga caacgtggag ccacgcctca tcaatccgcg cctcatgggg ggcaacaggc
    10681 ggctgaatca ccgccggagg ttcggcagtg gggagaggcc ccggttcaag gtcaggccta
    10741 ggctccggtg aaggggcggg aaccggggtc ggcgccggag aaggcgccgg gacgggagcg
    10801 gagaattcct gttctgtgcg atgctcaagt atccggtacc actgccacgc ggaaacagta
    10861 agtaccaaca ggacgatcag aacagctacc tgcttcgccc actcaggtac ttttcttttg
    10921 ccataaaaca tgaaaggagc ctcctcatcg ttcggtgcgg aagtttttca acagaatttg
    10981 attctaccat ggaaacggaa agaagaaaga gaggaaaata cttaccgttt ttgctcgttt
    11041 tccttcggtt cccccttgca tttctgactc gaaggtcgta cactgcataa gtttttaaat
    11101 tacccctaag gaggaggtag agacgtggaa aacgagtttc agttcgacct cgaacgttac
    11161 atgtcccaag ctaatttcaa taggataaag gattttgcac aagacaaaga aacgcctttt
    11221 cttattgtgg acttgaaaaa ggtcgaggaa aagtacgatg aacttcttga ttcgctgcca
    11281 tttgcgaaga tctattatgc cgtaaaggcg aacccccttg aacaggtgct ccgtatgctt
    11341 gtgcagaagg ggagctgctt tgatatcgct tccatctacg agctggatca cattcttgct
    11401 ctcggcgctt cgccggacag aatcagttac ggcaatacca taaaaaaagc gcgaaacatc
    11461 gcgtacgcat actccaaagg agtccgtctt ttcgccacgg attctgagag cgacctgcga
    11521 aagttggcga aaaacgcccc cggagcacga gtttttttcc gcatcctgac ggatggaagc
    11581 ggcgccgact ggcctctctc cagaaaattc ggcgcgcatc ctgacactat tttcaagctt
    11641 atcctcttgg ctgcaaaact cgggctcgaa ccgtacggca tttctttcca tgttggttct
    11701 cagcagcggg acatcggtca gtgggacaac gcaatctccg cttgcaaata cctcttcgat
    11761 tgcgccgaag ctgaaggaat tactttaaaa atgctcaatc tcggcggggg attccctgcg
    11821 acatacatct cgccgacgca tgagacttcc gtatatgctg cggaaatcat ccgttttctc
    11881 aaggaagact tcggagaaaa tcttccggaa atcatcctcg aaccggggag atttatggca
    11941 ggagacgcag gcgtccttgt cacggaggta gtccttatat cgaagaaatc ggaagcgaac
    12001 cagtattcct gggtatatct cgacgccggc aagtttggcg gattgattga gacgcttgat
    12061 gaatccatca aatacccgat ttactcggag aagaaaggcc ccgtccagga gaccattctc
    12121 gcgggaccca catgcgacag catggatatt ctttacgaaa atgaaaagta cgcccttccg
    12181 gcctctcttg aagagggcga ccgcctctac tttttcacca ccggagcgta tacgcagaca
    12241 tactcctccg tctgtttcaa cggctttcct cctcttaaag cgtacgttct tgaaaaataa
    12301 aggaagttct cttatactga cataataatg tccatcgggg acgggaagat acccgccccc
    12361 gatgttctat tgacggcgtt cttcgtgaaa ggaggcgtgt cccgtggaaa ttcgtcaaaa
    12421 acggacaaac gaactttggt tcaccgagga acagagtgta aacatgaaac tctctcttcg
    12481 cgtgtcggat gttcttttga atatcaccag cccctatcag gatatcttgc tcattcaaac
    12541 cgctgagtac ggaaggatgc tggtgcttga cggcgcaatc cagattactg aacgcgacga
    12601 gttctgctat tcggagatga tggcgcacgt tgcgctctgc tcccatccat ctccgaaacg
    12661 agtgcttatt gtcggcggcg gagacggcgg tgtgctccgc gaagtgctcc gccacaaatg
    12721 cgtcgaaaaa gcgacgctcg ttgatatcga cgaggaagtg atcaacgcat caaaaaaatt
    12781 tctgcccacg atcaacgtgg cgcttgagga tccccgcgcc gatgtgcgtc ctatggacgc
    12841 aatcgagttt atcgcatcaa gcgacgacga attcgacgtc gccatcgttg acagtaccga
    12901 cccggtcgag tttgccgccg ggttgttccg ttcccctttc tacaggggta tttttaaggc
    12961 gctgaaaaag gacggtatgg tagtcgccca gacagaatca ccattcaccg acgcatccgt
    13021 tgtcaagggg gctttcaagg aaatgagcgc tgttttcccc atcgtgcgga tgtgctgggg
    13081 agcgatgccc acctatccaa gcggtatgtg gacctacacc ataggctcga agactgtcga
    13141 cccggccgat atacgcagac cttctcccga tggaacaaaa tactatacgg aggacattca
    13201 caaagcggct ttcgtgcttc cgccgtttgt taaagacttg ctgaaataag gcgtatactc
    13261 tataatatag aagtcaacta cgacattcag gagggaattt tgcagagatg agagtattgg
    13321 tcgcccttgg gggcaatgcc attcttcaaa gagggcaaaa gggaaacgcc tcggaacagc
    13381 gtgcgaacgt ccaaaagacc gtgacgcaaa ttgtcagaat gatccatgcc gggcacgaag
    13441 tggtcgtgac gcacggaaac ggccctcagg tcggagcaat tctcatacag aacgaactcg
    13501 ggagcgcctc tgttcccgcg atgcccatgg atatatgcgg cgccgagagc caggggctca
    13561 tcggatacat gttctgccag agcatacaga ataccctgct tgcggacggc atcgaaggac
    13621 acgcgcctgc ttgccttgtt acccgagtcg aag
//
LOCUS       DAOPKU010000010        13529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_4780_length_13529_cov_5.085053, whole genome shotgun sequence.
ACCESSION   DAOPKU010000010 DAOPKU010000000
VERSION     DAOPKU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4780_length_13529_cov_5.085053"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..933
                     /gene="rsmA"
                     /locus_tag="PK849_00660"
     CDS             1..933
                     /gene="rsmA"
                     /locus_tag="PK849_00660"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPE64660.1"
                     /translation="MVGGSNPLVPTRILKRGHTPAFFVFVEERKDRMGREQKGFKNKK
                     SLGQNFLVDRNILRHILEQADLSVDDTVLEIGAGQGVLTRELAASPCSFVFSVEIDEG
                     LSEYLRDITLLYPNTFSLLWGDALAVDYSSLSPVPTKVVANIPYNITTPLLWRLLEQL
                     PEAGTGYFLLMVQKEAAERITAPPGTKERYPLGITLELMGKARSVRTVHPQSFRPVPA
                     VESSLLEIRLSGMHATLPKDSLWRNLLRSSFAQRRKKLSNNLRGFYPSVSWSEAFERT
                     EIHENARAEELTGEQWLALWFFLKTFEKKSLSPE"
     gene            complement(966..1250)
                     /locus_tag="PK849_00665"
     CDS             complement(966..1250)
                     /locus_tag="PK849_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPE64661.1"
                     /translation="MYTVTKAELITEVAKATGLNKKESGAAVGAVFEAIEAALEKGEK
                     IQLVGFGTFEVRERAAREGRNPQDPKKVIKIPAKKIPVFRPGKALKDKVE"
     gene            complement(1380..2696)
                     /gene="der"
                     /locus_tag="PK849_00670"
     CDS             complement(1380..2696)
                     /gene="der"
                     /locus_tag="PK849_00670"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPE64662.1"
                     /translation="MPVVSIIGRPNVGKSSLFNRLLGRREAIVDDMPGVTRDRLYGEA
                     EWRDRKFFVVDTGGFLARDENEFVEGMRVQVKLAIEESDLVLFVIDGREGPVWMDEDV
                     ADMLRKSGKPVIVVANKIDDGVHEDDVFQAYTLGFEEVIGISAEHKRYIYDLQDLIVS
                     KLPPEKEQPSETGEIRVAIVGRPNVGKSSILNSLAGEERALVSNIPGTTRDALDTLVT
                     INGTVFNFIDTAGLRRKSRVENDIEYYSFVRTLQAIDRCDVALLVLDAEEPFTDQDKK
                     LAALVVERGKGIVLLLNKWDLLGNNEKLGDSMRKKFRDEMVFLQYAPFAFISALTGRG
                     MHRISEQILHVAENRNRRIGTTQLNRLVRDILAFDTLPTDQKGRPFKIYYCTQSGVEP
                     PTFIFFVNHPDLASPAFENHMEKALRGLEDFSGTPIRIFWRGKEEK"
     gene            complement(2720..3631)
                     /locus_tag="PK849_00675"
     CDS             complement(2720..3631)
                     /locus_tag="PK849_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64663.1"
                     /translation="MPLTHTLLSETKRCLASGKTALWLLDRKGYAGEIACEDCGNAAR
                     CPKCGTAMVWEEKNDRLRCPACASLRSIPQVCPVCRGHFLIGKRPGLEALFPVAKSLE
                     SADQPTLFLEDFKTAGKGAVRSLAGRLSSGGIVVGTRAALALCDIVNTGFAGWIDVDA
                     EVRSIAYQAKFTVFSMVWESLWRGMNHSERVVLLQSRRPGAGWQKGVVQGWKAFWNHE
                     LEERKELGLPPYSYLLEIKTSSLQQKSDMISRMEERGLFPMDPGDPPLHFWVTVCSVS
                     DVQDIIAPFFSIGCSRNGFPEVTVWID"
     gene            complement(4550..5767)
                     /gene="coaBC"
                     /locus_tag="PK849_00680"
     CDS             complement(4550..5767)
                     /gene="coaBC"
                     /locus_tag="PK849_00680"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPE64664.1"
                     /translation="MPTWKNKVKIVLGITGGIAAYKAPELVRTFVKSGCEVEVVLTED
                     GEKFVSPMVLSTLAGRRIWRQRDFLSDEYGWRIPHISLADWADVVVVAPCTAETLANT
                     VQGRGKELLGSLLLATRAQVLLCPAMNVNMLSNPATAENIRTLKNRGYAVVDPDSGDL
                     ACGYEGKGRLPSPNVLLEEMWRVACPKKELQRKNVLVTAGPTWEFLDPVRFLSNPSTG
                     KMGVAVARAAWYRGASVTLVLGPVSLTNLSGFEVVRVVSAEEMKEAVLSRSGEMEYIV
                     KAAAVGDYRPASYAGEKIKRSGSDTLRIDLIQNTDIAAALGERKRPGQVLIGFAAESH
                     DLQKNASEKIRKKNLDFIVANDITAPAAGFGADTNTVRILSADGSVSEFAGTKEDVAE
                     EIWNTVLADKQLS"
     gene            complement(5748..6002)
                     /locus_tag="PK849_00685"
     CDS             complement(5748..6002)
                     /locus_tag="PK849_00685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPE64665.1"
                     /translation="MKFYDLDSLAEKQGLDNKYILTAAVAARARSLSEQKGRTLEEDN
                     EKFISTALRDFDLGLVRLSSDERESEEAPVAVVSDAHVEE"
     gene            complement(5999..6586)
                     /gene="gmk"
                     /locus_tag="PK849_00690"
     CDS             complement(5999..6586)
                     /gene="gmk"
                     /locus_tag="PK849_00690"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPE64666.1"
                     /translation="MKKRTTGSLFVLSGPSGAGKGTLRKKMFEKVPGITFSISCTTRE
                     PREGEREGIDYHFISADEFQSRLERGEFLENADVHGHLYGTLREDAVRVLNSGSDILL
                     EIDVQGALQVREKMPGSILLFVAPPSFEELEQRLRDRGTESEERLQIRLRNARWEMEK
                     APLYDYVIINDDIERAAEELEGIILEQRKNREARR"
     gene            complement(6564..6833)
                     /locus_tag="PK849_00695"
     CDS             complement(6564..6833)
                     /locus_tag="PK849_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HPE64667.1"
                     /translation="MTGRLVHIGFGNMLVAERIVAIVHPSSAPIKRLKEEAREDGRLI
                     DATQGRKTRAVLITDSNHVVLSAIQPETIVNRYEEEAEDEKTNDG"
     gene            complement(6841..7725)
                     /locus_tag="PK849_00700"
     CDS             complement(6841..7725)
                     /locus_tag="PK849_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPE64668.1"
                     /translation="MIRSMTGFTRETRAFEWGTLTVELSSVNHRYQEISPRLPREMAS
                     LEPLITGRLRQGVGRGKIRCSVELHWAPQFRTATLDATVLSNYHTQLAELAERLGTGE
                     TLPLASLLSLPGVFESPSVLAGIEDELNAALESLLEQAIEHLQAMRSTEGTNLLHAIE
                     EYLNAFESLLNSLEKAWDPKRDQAIEDMRSRITNLLEAMSIEVDQNRVAQEVVILSDK
                     WDIAEEFVRSRSHLKQFAAVLNGKSSEGRKLDFLLQEMNREINTMGSKISDAELRWMV
                     VEAKSLLEKIREQVQNVE"
     gene            complement(7722..8825)
                     /gene="hflX"
                     /locus_tag="PK849_00705"
     CDS             complement(7722..8825)
                     /gene="hflX"
                     /locus_tag="PK849_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPE64669.1"
                     /translation="MRKAILAAIELPKQEFAPTVLLDELTLLLENLGIQTAGTVIQRR
                     ERPDSAFLLGKGKVDEIALFCKAAGADLVVCNESLTPGQKSNLQHTIGLEVWDRPFVI
                     MKIFEKRARTSEAKLQVEMALCRYEIPHLKGLGAQMSRLGGGIGTRGPGETEFERHRR
                     KLERRVRDIGRKLEAMKHKRALQRDRRRKMDVPVVSLVGYTNSGKSSILRALSGDTSL
                     IAENRLFSTLDTFMRRVDLPSGRTVLLSDTVGFIRDLPPGLVTAFLTTLEEIVASTFL
                     IVVLDISTTDVQEIRDVVEHTLHEIGAGEIPRLFVLNKIDLTSPEYLDATTARLGIEN
                     SQSIATSVLEKRGLHELLDSMDLFLEKMEVSQI"
     gene            complement(8822..9478)
                     /locus_tag="PK849_00710"
     CDS             complement(8822..9478)
                     /locus_tag="PK849_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPE64670.1"
                     /translation="MMTYWNRVFYFFVKWFFFIALKIYNRLSIRWTSSLPQRNVIVVA
                     NHASNLDPVIMGAVFPRRLRYLAKEELFKPFIFGNMVRALGSIPVRKSDSQSAGTALR
                     TFLKLLESGENVLIFPEGSRSLNGELQPLEGGVALIALKSGAPIIPSFISGTFEAMPP
                     GSRWVKPLHLSIVFGDTIDSAKYIPFGKEGREMLLKDLNEVFISLSTADLPGKSRNNS
                     "
     gene            complement(9483..10151)
                     /gene="cmk"
                     /locus_tag="PK849_00715"
     CDS             complement(9483..10151)
                     /gene="cmk"
                     /locus_tag="PK849_00715"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPE64671.1"
                     /translation="MNCVSFVAAIDGPAGAGKSTLARKVANLLRMRYLDTGALYRALA
                     FSLYSSQVSPEEGPEMEAAIAGISVEILENGLLLNGKEVGKEIRSPLVDSIVSSYAAL
                     PSVRKRLLSIQREQGKHGSLVADGRDMGTVVFPDADVKIFLTASDEVRAKRRLLELRE
                     RGEDVTYEEVLQTIRERDRVDSERSTAPLRKAEGAIEVNTDALSIEEAVAALASIISK
                     RKGA"
     gene            complement(10148..11038)
                     /locus_tag="PK849_00720"
     CDS             complement(10148..11038)
                     /locus_tag="PK849_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPE64672.1"
                     /translation="MEGIDERSRELIKTRILGKIKEIRSFPQFVVETLRKLNDPDSSA
                     HDVAASLSRDEGLVIRTLKLANSAAYGISRDISSVAEAIALLGYKNISSIVLAASVYS
                     VMDKPLAGYALDRGMLWRHSLTVAYSSRYIAQLTKKGLPEEAYVGGLLHDIGKVVLND
                     YVRFGYGIIVKMVEEQQIPFLEAEIQVLGFDHAMVGAMLVEKWGLPEPYKYAVAHHHS
                     PNNLAEPKHQNLVDVVHLANSLCLMLGVGIGADGLQNYLYPEVLERLGISDYEMLMSD
                     LVDFVAQAEAELSEMEEAFQ"
     gene            complement(11060..11788)
                     /locus_tag="PK849_00725"
     CDS             complement(11060..11788)
                     /locus_tag="PK849_00725"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPE64673.1"
                     /translation="MENGTRLNRFLAMCGIGARRKVEDVIASGRVRINGKVVLLPGYR
                     VENDMDVSVDGQTVQPQEHRYLVMNKPDGVVCAVEDGYDPTVLGLLPPEFQTVRLFPV
                     GRLDRESQGLLILTNDGAFAQEILHPSKRILREYEAMLDHAITRKEVRKWMAGSLLDG
                     RLIKPVNVLLLDKEPAGCWVSVVLAEGVKREVRRMAAEIGCDVVVLVRKRIGKMELKN
                     LQQGQFIEMSRGSLWNAIRSGGVV"
     gene            complement(11838..12446)
                     /gene="scpB"
                     /locus_tag="PK849_00730"
     CDS             complement(11838..12446)
                     /gene="scpB"
                     /locus_tag="PK849_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPE64674.1"
                     /translation="MTSSLPRFETLSSTAKIIEAILFAAPSPVSVNEMATLLSLSPSA
                     VKSELEDLRSYLLRHEHGITVIESAGGWRMATAEDVSEIVGAFRETAIAQKIRLSKAA
                     VETLAVIAYNQPVTRSEIEELRGVRCERVIETLLSHALIRIAGRRKATGSPLLYRTTD
                     RFLETFGVLSISELPTLEELDELAPVDEPHFVPVDEEERTNG"
     gene            complement(12421..13194)
                     /locus_tag="PK849_00735"
     CDS             complement(12421..13194)
                     /locus_tag="PK849_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPE64675.1"
                     /translation="MEERLGELAEFQAEIDGFSGPFDLLCWLVESRELEAASISVGQV
                     VRIYGAYLANTNKVSVSVVSEFLFMAASLVLNKIRALLPGRESLYESEEPENDPGEDV
                     LEKLSRYRPYRSAARDLAILKEERDRFFFRSAPEEEDDEPSFDLGDLFALCRLWWSLR
                     DLKRRTKNAAPYIFENEDDWSGVPSSLPEEEQIERKISEILSELNSDGSLRLSFLLRE
                     NRAMANFVVTLLALLEMSRMGRIRILQEELFDDIVITPL"
     gene            complement(13197..>13529)
                     /locus_tag="PK849_00740"
     CDS             complement(13197..>13529)
                     /locus_tag="PK849_00740"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048480.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPE64676.1"
                     /translation="DPARERRTDPGDPEKCPVWDIHKVFNNNEDEKNEIASGCRSAGI
                     GCVDCKKKLQAHVERVMGPIQERRTFYEKSPDLLSDILREGAEKAGEVARRTIDEVIN
                     AIGFFKRG"
BASE COUNT         3229 a         3720 c         3249 g         3331 t
ORIGIN      
        1 gtggtcggag gttcgaatcc tctcgtcccg accagaatat taaagcgggg gcataccccc
       61 gctttttttg tatttgtcga ggagcgaaaa gacagaatgg gaagagaaca aaagggtttt
      121 aagaataaaa aaagcctcgg gcagaacttt cttgtcgacc ggaatatact tcgacatatt
      181 ctggaacagg ccgatctttc cgtggacgat accgttctgg aaataggagc cggccaagga
      241 gtgctgacaa gggaacttgc ggcctctccg tgctcgttcg ttttctctgt agaaatcgat
      301 gaagggcttt cggagtatct gcgggacatc actctgctct atccaaacac gttttctctt
      361 ctgtggggag atgcgcttgc cgtggactat tcttcccttt ctcccgttcc aacgaaggtt
      421 gtagcaaaca tcccatacaa cattaccact cccctgctct ggaggctcct ggagcaactc
      481 ccggaggccg ggacgggcta tttcctcctt atggtacaga aagaggcggc agagaggatt
      541 acagcccctc caggaacaaa ggaacgctac ccccttggga taacgctcga gcttatggga
      601 aaggcgcgaa gtgttcggac cgtccatcct cagtctttcc gtccggttcc tgctgttgaa
      661 tcatcgcttc ttgaaatccg tctttccgga atgcatgcga ctctcccgaa ggatagcctc
      721 tggaggaatc tcctccgttc cagttttgct caaaggcgta agaagctctc gaacaatctg
      781 cgcggttttt atccttcagt tagctggagc gaggcttttg aacgtacgga gatacatgaa
      841 aatgcacggg ccgaggaact gacgggagaa cagtggttgg cgttatggtt ttttttaaag
      901 acctttgaaa aaaagagcct ctccccggag tgagcgggaa gaggcaaaaa aggaaggaaa
      961 gaggcctact ctaccttgtc cttcaaagct ttgccgggcc ggaaaacagg aatcttcttc
     1021 gcggggatct tgataacttt cttcggatcc tggggattac gtccttcacg agccgctctt
     1081 tcccggacct caaacgtacc gaaaccaacg agctgaatct tctcgccctt ttcaagagcg
     1141 gcttcgatcg cctcaaaaac cgctcctacc gccgcgccgg actccttttt gttcaagccg
     1201 gtcgccttcg ctacttctgt gatgagttct gccttggtca ccgtatacag cctcctccaa
     1261 atatgatatg aatctgaaac tgactccgaa cctggttcgt tttgataaag ccttgcggta
     1321 tccgaaccta tcagttcttt attattacca gcaactgcaa ttccggtcaa gcggaatctt
     1381 catttttcct cttttccacg ccaaaaaatg cgaattggtg tgccagaaaa gtcctccaga
     1441 cctctcaagg ccttctccat atggttctca aaggccggag aggctaggtc cggatggtta
     1501 acgaagaaga taaatgtagg aggttctacg ccggattgcg tgcagtaata aatcttgaac
     1561 ggccgtcctt tttggtcggt gggaagcgta tcgaacgcaa gaatgtcccg tacaagacgg
     1621 ttcaattgag tggtaccgat tcttctgttt ctgttctctg cgacatgaag aatctgttcg
     1681 cttattctgt gcatccctct tccagttaat gcggaaatga atgcaaacgg agcgtactgt
     1741 agaaagacca tttcatcgcg gaattttttt ctcattgaat ccccgagttt ttcgttattc
     1801 cccagcagat cccatttgtt gagcagaagt acgatccctt taccgcgttc gacgaccaga
     1861 gctgcgagtt ttttgtcctg atcggtaaaa ggttcttcgg cgtcgagaac aagtaaagcg
     1921 acatcgcagc gatcaatcgc ttgcagggtt ctgacaaacg agtagtattc aatgtcgttc
     1981 tccactctgc tttttctgcg gagtccggca gtatcgatga agttgaaaac agttccgttg
     2041 atggtaacga gcgtatctaa agcgtcgcga gtcgtgccgg ggatattgct caccaacgcc
     2101 cgctcttctc cggccaaact attcagtatg cttgatttgc caacatttgg acgaccgaca
     2161 atcgcaacgc ggatttctcc ggtttccgaa ggctgctcct tttcgggggg cagttttgaa
     2221 acgataagat cctgaagatc atagatatac cgtttgtgct cggcgctaat acctatgacc
     2281 tcttcgaagc cgagagtgta ggcctgaaag acgtcgtcct catgaacgcc gtcgtcgatc
     2341 ttgttagcaa cgacgatgac tggttttcct gatttccgaa gcatgtccgc cacgtcctcg
     2401 tccatccaga cgggtccttc cctaccgtct atgacgaaga gtacaaggtc gctctcttcg
     2461 attgccagct tcacctgcac gcgcattcct tctacgaact cattctcgtc ccgcgcaaga
     2521 aatccaccgg tatcaacgac gaaaaacttt ctgtcgcgcc attccgcctc tccgtaaagc
     2581 ctgtctcttg ttactccggg catatcgtcc acgattgcct ctcgtcttcc tagaaggcga
     2641 ttgaacagcg acgatttgcc aacattcggt cttccaatta ttgatactac tggcattgcc
     2701 tcttcctccc cgataaagct caatcaatcc agacggtaac ttcaggaaaa ccatttctgg
     2761 aacagcctat ggaaaaaaac ggagcgatga tatcttgaac atccgatacc gagcacacag
     2821 tcacccaaaa atgaagcgga gggtcgcccg gatccatcgg aaagagtccc cgctcctcca
     2881 tccgtgatat catatcggac ttttgctgaa gcgatgaagt ctttatttcc aggagatagg
     2941 agtacggagg aaggcccagc tcttttcgtt cctcgagctc atggttccaa aacgctttcc
     3001 atccctgcac aacgcccttt tgccagcccg ctcccggcct tctgctttgt aaaagaacga
     3061 ctctttccga atggttcatt ccccgccata aggactccca gaccattgaa aaaacagtaa
     3121 atttcgcctg gtaggcgatg ctgcgcactt cagcgtcaac atcaatccac cctgcgaagc
     3181 cggtattgac tatatcacat aaagccagcg ccgcacgtgt tcctacaaca atacctccgg
     3241 aggagaggcg accagcgaga gaacgtacag cgcctttccc ggcggtttta aaatcttcca
     3301 gaaaaagtgt aggctgatct gcggattcca aactcttcgc aaccggaaaa agagcttcca
     3361 gtcccggcct ttttccgata agaaagtgcc ctctgcacac aggacacacc tgaggaatgg
     3421 aacgaagaga tgcgcaggca gggcagcgca gacgatcgtt tttttcttcc cagaccattg
     3481 ccgttccgca tttgggacaa cgtgcggcat ttccgcagtc ttcacaggcg atttctccag
     3541 cgtatccttt cctgtcaaga agccagagcg ccgtcttccc tgaggcaaga caccttttcg
     3601 tctccgagag gagcgtatgt gtcaacggca aagagtcccc cactccctga acagaggaag
     3661 caaacgcctc ccgaaggtcg acgatcctga cattattccg tgccgggcgt tcgttagact
     3721 gcggtttccc gcgcagaaaa atacgggaag aggggacccg ccccgagagt acaagcaagg
     3781 cggactccag cctggctctt ttgccggcaa gagtccgtgc attaacgaac ggttttttat
     3841 aggagcgata tgctccgctg ctttcctcat caacaataat ggtttcaatc tgttgtaaag
     3901 gggcaaaaac tgcgccgggc cccccgatga ttcctctcgg tatattattg cgcgcttgaa
     3961 tccatgccgc aaggagtttc ttccctccct gggacggcca gagaatggtc tctttctttt
     4021 gagactcgga aagctgcttc caaaacgcgg aggcaagtcc ctgctcaggg aaaagaacaa
     4081 gaaaacgctc gcttctttcc agaagagaga ggaatttgtt acaacgctct tggaaactac
     4141 acgcataaaa aaacaactct tcctgagcct tggtctccat ccgaggcaat ggaagaggta
     4201 cgggagtggc atccgagggt gaaaggaaag cggacggaat tccgatacga agcagttctc
     4261 cacttccgca aagaaacact cttcctcccc agtcaaaaag ctcccataaa ggcggtggga
     4321 aaaaagaaac gtcgtcaata atcctcttta tgcgccgaat cgagaacgcg ccgtctccct
     4381 cctgaacagc gttgctgagg cgctgcacaa aaccgatacg ctctccacgt cctaaaggaa
     4441 cgacgactcg tgcgccgagc ggtacgttca actctgtcgt atacgtcaga ctgttccacc
     4501 atgggcctgg aacgagaacg tcataatatg gcatggaggc gcctcttctt cacgataact
     4561 gtttgtctgc gagaacggta ttccagatct cttcggctac gtcttccttg gtccctgcaa
     4621 actccgatac agagccgtcg gcactcagta tgcggacagt gttggtatct gcaccgaagc
     4681 ctgctgcagg ggctgttata tcgtttgcga caatgaagtc taaatttttc tttcgtatct
     4741 tttcggaggc atttttctga aggtcatggc tttcggcagc aaacccaatg agcacctgcc
     4801 ccggtctttt tcgttcaccg agggcggcgg cgatgtcggt attctggatg agatctatcc
     4861 gaagcgtatc cgacccgctt cgttttatct tttctcctgc ataggaagcg gggcgatagt
     4921 cccctaccgc agctgctttt acaatgtact ccatttcccc ggagcgtgag aggacagcct
     4981 ctttcatctc ttcagcggaa acgacgcgaa ctacctcgaa tcccgaaaga ttggtcaagg
     5041 agaccggacc aagcacaagg gtgacggaag ctcctctgta ccaggcagcc cgggccacgg
     5101 cgacccccat ctttccggtg ctcggattac tgagaaaacg caccggatca aggaactccc
     5161 aggttggtcc ggcagtgaca aggacatttt tcctttgaag ctctttcttg ggacaggcta
     5221 cacgccacat ttcctcaaga agtacattcg gggagggaag gcggcctttc ccttcataac
     5281 cgcacgccaa gtctccggag tcgggatcga caaccgcata ccccctgttc tttagagttc
     5341 ggatgttttc cgccgtggca ggattggaga gcatattcac gttcatcgcc ggacagagaa
     5401 gcacctgcgc ccgagtcgcg agaagaagag aaccgagtag ctctttacct cttccttgaa
     5461 ctgtatttgc aagcgtttcc gccgtacaag gagcgaccac cacaacgtcc gcccaatccg
     5521 ccagcgaaat gtgaggaatc ctccatccgt attcatccga caaaaaatca cgctgacgcc
     5581 agattcttct tcccgcgagg gttgaaagca ccatcggact gacgaacttt tctccgtcct
     5641 cggtcaacac aacttcgacc tcgcacccgg acttcacaaa ggttcgtaca agctccgggg
     5701 ctttgtaagc ggcgattccg cccgttatgc cgaggacgat ttttacttta ttcttccacg
     5761 tgggcatctg agacaacggc aacgggagct tcttcgcttt cccgctcatc tgatgaaagg
     5821 cgcactaggc caagatcaaa atcccgcaga gctgtcgaga tgaacttctc gttgtcctct
     5881 tcaagcgttc gacctttctg ctcgctgagc gagcgcgctc ttgccgctac tgctgccgtg
     5941 aggatatact tgttatccag accctgcttt tctgcgagag aatccaaatc ataaaatttc
     6001 atcttcgcgc ctccctgttc tttctctgtt caaggatgat accttcgagt tcttctgcgg
     6061 ccctctcaat atcatcgtta attatcacat aatcatataa cggcgccttt tccatttccc
     6121 atcgagcatt tcgaaggcga atttgtaaac gttcctctga ttccgttcct ctgtcacgaa
     6181 gacgctgttc aagctcttca aacgagggtg gcgcgacgaa cagcaggata cttccgggca
     6241 tcttctccct gacctgcaaa gccccctgaa cgtcgatttc caagaggatg tcgcttcctg
     6301 aattcagcac acgcaccgca tcttcgcgca aagtaccgta taagtggccg tggacatccg
     6361 cattttcaag aaattcgcct ctctcaagac gtgactggaa ctcatccgca gaaataaaat
     6421 gatagtcgat tccttcgcgc tcaccttcgc gcggctcgcg ggtagtgcac gaaatcgaga
     6481 aggtgatacc gggcaccttc tcgaacattt ttttccggag tgttcctttc cctgccccgc
     6541 taggacctga gaggacaaaa agactacccg tcgttcgttt tttcatcttc agcctcttct
     6601 tcgtatcgat tgactatcgt ctctggctga atcgcagaga ggacgacatg attgctgtct
     6661 gtaatcaaaa ctgcccgtgt ctttcggcct tgcgtcgcgt caatcaggcg accgtcctct
     6721 cttgcctctt ccttcaggcg ctttatagga gcggaggacg gatgcacaat agcgacgata
     6781 cgttcagcga caagcatatt gccaaaacca atgtgaacga gccttccggt catattctta
     6841 tcactccacg ttttgtactt gttctctgat cttttcaagg agcgacttcg cttcaacgac
     6901 catccaccgg agttcagcgt cggaaatctt cgaccccatg gtgttaattt cccggttcat
     6961 ctcttgaaga agaaaatcga gtttgcgccc ttccgatgat tttccgttga gcacggcagc
     7021 aaactgtttc aggtggcttc gtgaacgaac aaactcctcg gcaatatccc acttgtcgga
     7081 gagaataaca acttcctgag caacccggtt ctggtcaacc tctatcgaca tggcttccag
     7141 aaggttcgta attcttgatc gcatatcctc gatagcctga tctcgcttgg ggtcccatgc
     7201 tttctccaaa gaattgagga gggattcaaa cgcgttgagg tactcttcaa tggcgtgcaa
     7261 gagatttgtt ccctccgtac ttcgcatcgc ttgaagatgt tctatggctt gttccaacag
     7321 agactcaagc gctgcgttca gttcatcttc gatgcctgcg agaacggaag gagattcaaa
     7381 aacgccgggc agcgataaaa gcgacgcaag aggaagagtt tctccagttc cgagacgttc
     7441 ggccaactcc gcaagctgtg tatgataatt cgaaagcaca gttgcgtcaa gtgtggcggt
     7501 tcgaaactgc ggagcccagt gcaattccac agaacaacga attttacctc tgccaacgcc
     7561 ttgtctcagt cttcctgtga tcaaaggctc caatgacgcc atctcccgag gcagacgagg
     7621 tgaaatttcc tgatatctat gatttaccga ggagagttcc acggtgagcg ttccccattc
     7681 aaaagcgcgg gtttcccgcg taaatccggt catgctgcgt atcatatttg ggagacctcc
     7741 attttttcaa gaaaaagatc cattgaatcg agtagttcat gtaatcctcg cttttcaagg
     7801 acgcttgtcg cgattgactg cgagttttca atgcctaagc gagccgttgt cgcgtccaaa
     7861 tattctggtg aggtcaggtc gatcttgttc aagacgaaaa gacgaggaat ttcccctgcg
     7921 cctatttcgt gcaaggtatg ctctaccacg tcccggattt cttgaacgtc ggttgtagag
     7981 atatcgagaa caactatgag aaatgtagat gcgactatct cttcgagagt cgtaaggaac
     8041 gctgttacca gaccgggagg aagatctcgt ataaacccga ccgtatcgga cagaagaact
     8101 gtcctcccgg aggggagatc aaccctgcgc atgaacgtat ccagtgttga gaaaagcctg
     8161 ttttccgcga taagcgaggt gtcgccgctt aatgctctca ggatcgaaga ttttccgctg
     8221 tttgtataac cgaccaaaga aacaaccggt acatccatct tcctgcggcg atctctttgg
     8281 agcgccctct tgtgtttcat cgcctccagt tttctgccga tatcccggac tcttcgttca
     8341 agtttccgac gatgtctttc aaattcggtt tcacccggac cgcgtgttcc gatacctcct
     8401 ccaaggcgcg acatctgcgc tcccaaccct ttgagatgag gaatttcgta tctgcacaac
     8461 gccatctcga cctgcagctt tgcttcggac gtcctcgcgc gcttttcgaa tatcttcatg
     8521 atgacaaaag gacggtccca gacttccagg ccaattgtat gctgcagatt actcttctgt
     8581 cccggggtga ggctttcgtt acaaacaacg aggtcagccc ccgcagcttt acaaaaaaga
     8641 gcgatttcgt cgactttccc cttgccaaga agaaacgctg aatccggtcg ttcgcggcgc
     8701 tgtattacag tccctgccgt ttggattcca agattttcaa gaagaagcgt cagttcgtcg
     8761 aggaggactg tcggagcaaa ctcctgcttt ggaagttcaa tcgccgcaag gattgctttt
     8821 ctcatgaatt gttcctggat ttgccaggca gatcagcagt gctcaaagag atgaaaacct
     8881 cgttaagatc cttgagcaac atctctcttc cttctttccc gaaaggaatg tatttcgccg
     8941 aatcaatggt atccccaaag acaatcgaaa gatgaagagg ttttacccag cggcttccgg
     9001 ggggcattgc ctcgaaagta ccggagatga aagagggaat gatcggcgct ccggatttta
     9061 acgcaatgag cgccacccct ccttcgagag gctgaagttc cccgtttaga gaacggcttc
     9121 cctccggaaa gatcaatacg ttctccccgc tttccaggag tttcaggaaa gtgcggaggg
     9181 ctgtccctgc gctctggctg tcgctctttc gtacgggtat ggagccaagc gccctgacca
     9241 tattgccgaa tatgaacggt ttgaagagct cttccttcgc aagatagcga agacgccgcg
     9301 ggaatacagc gcccatgatt acgggatcca gattgctcgc atgattggcg acaacaatga
     9361 cgttgcgttg agggagtgaa gacgtccatc tgatggacaa acggttgtag atcttcaagg
     9421 caatgaagaa aaaccatttg acaaaaaaat agaaaacgcg gttccaatac gtcatcatcc
     9481 ggtcacgctc cttttcgttt tgagatgatc gaagcgagcg ccgctacggc ttcttcgata
     9541 gaaagagcgt cagtattcac ttcgatagct ccttccgctt tccgaagcgg cgcggttgaa
     9601 cgctcgctgt cgactctgtc gcgttcgcga attgtttgca gcacttcctc ataggtcaca
     9661 tcttcgcctc gttcacgtag ttcaagaagg cgacgcttcg cacgcacttc gtcggaagcc
     9721 gtgaggaaaa tcttgacgtc ggcgtcaggg aagaccacag tacccatatc ccggccgtcg
     9781 gcgacgagag agccgtgttt tccctgttca cgttgaatcg agaggaggcg tttccgaact
     9841 gagggaaggg cggcataaga agatacgatg gaatcaacaa gaggagaacg aatttctttc
     9901 cccacttctt ttccgttgag caaaagaccg ttttcaagaa tttccacgga gataccggcg
     9961 attgccgctt ccatttcggg gccctcttcc ggcgagactt gactcgagta aagagaaaac
    10021 gcaagagccc tgtagagcgc gcccgtgtcg agatagcgca tacgaagaag gttcgcaacc
    10081 tttcgtgcaa gggtgctttt ccctgctccc gccgggccgt caatggccgc gacgaaagag
    10141 acgcaattca ttgaaaagct tcctccatct ccgataactc cgcctcggcc tgagctacga
    10201 aatccacgag atccgacatg agcatttcgt aatcggaaat accaagacgc tccagcactt
    10261 caggatagag atagttctgg agaccgtcgg ctccaatacc aactccgagc atcaagcaga
    10321 gggaattcgc aagatgcact acatccacga gattctgatg tttgggttca gccaggttgt
    10381 tgggagagtg atgatgggcg actgcatatt tatacggttc cggcaatccc cacttttcca
    10441 caagcatggc ccccaccatc gcatgatcga agccaagcac ctgtatttca gcctcaagaa
    10501 atggaatctg ctgttcctcg accattttga cgatgatgcc gtatccgaag cgaacgtagt
    10561 cgttcaaaac aactttcccg atatcgtgca aaaggccgcc gacgtaggct tcttcgggaa
    10621 gacctttttt tgtgagttga gctatatagc gggatgaata ggcgacggtc agggagtgac
    10681 gccacagcat accccggtcc agcgcgtagc ctgcgagcgg tttgtccatt acagaataca
    10741 ccgaagcggc gaggacaata ctgctgatgt ttttgtaccc gaggagcgca atagcctcgg
    10801 cgacgctgga aatatcacgt gaaatgccgt atgccgcgga attggcaagt ttcaacgtcc
    10861 gaatcaccaa gccctcatcc ctcgaaagac tcgcagcgac atcatgagcg ctgctgtcgg
    10921 gatcattcag ctttcgcaaa gtttccacca cgaactgcgg gaatgaccga atttctttta
    10981 tcttaccgag aatgcgcgtc ttgatcagtt ctcgggaccg ttcgtcaatt ccttccatct
    11041 tccgccctcc tccataccat cagacaaccc ctccggatcg gatcgcattc cacagagatc
    11101 ctcgggacat ctcgataaac tgcccctgct gcagattttt caactccatt ttaccaattc
    11161 ttttccgaac tagcacgaca acatcgcaac caatttcggc ggccatacga cgaacttcgc
    11221 gttttacccc ttcggcgagt acaacggaaa cccaacaacc ggctggttcc ttgtcaagca
    11281 acaaaacgtt gaccggcttg atcaagcgcc cgtcaagaag agatcccgcc atccatttcc
    11341 gtacttcttt ccgtgtaatg gcatgatcaa gcatcgcttc gtactcccta agaatcctct
    11401 tcgaagggtg caatatttct tgagcaaatg ccccgtcgtt ggtcagaatc aacagcccct
    11461 ggctttccct gtcaagacgt ccaacaggaa aaagccgaac cgtctggaat tccggaggga
    11521 gcaatccaag caccgtcgga tcatacccat cctctacggc gcacacaact ccatccggtt
    11581 tgttcataac gaggtatcga tgttcttggg gctgaacagt ctgtccgtca acgctcacat
    11641 ccatgtcatt ttcgacgcga tatcctggca gaaggacgac cttgccgttg atacggacgc
    11701 gtccggaagc gatgacatcc tccactttcc gtcgggcgcc tataccgcac atcgcaagga
    11761 atctgttcag tcgcgtcccg ttttccacgg cgtactcctc ctccgctcaa agagctttat
    11821 aaaatcttca ggggaaatca cccgttcgtg cgttcctcct cgtccacggg aacaaaatgc
    11881 ggttcgtcaa ccggcgcaag ttcgtcgagt tcctccaaag tgggaagttc cgagattgaa
    11941 agaacgccga atgtttctaa aaaacgatcc gttgtgcgat acaggagggg cgaaccagtc
    12001 gcctttcttc gtcccgctat ccggataagc gcgtgcgaga gaagcgtttc gatcactctt
    12061 tcgcaccgaa cacctcttag ttcctcgatc tcgctgcgcg ttaccggttg attatatgcg
    12121 atgaccgcca atgtctccac tgccgctttg ctgagacgga tcttctgtgc gatcgctgtc
    12181 tcgcggaagg ctcccacaat ttccgataca tcctccgccg tcgccatccg ccatcctcct
    12241 gcggactcga ttacggtaat gccgtgttca tggcgcagga gatacgaacg aagatcctcc
    12301 agctctgatt ttactgcgga aggagaaagc gaaagcagcg tagccatctc atttacagaa
    12361 acgggagaag gcgccgcgaa gaggattgct tcgattatct tcgcagtact tgaaagcgtc
    12421 tcaaagcggg gtaatgacga tgtcatcgaa aagctcctcc tgcagtatcc ggattcgtcc
    12481 catcctgctc atttcaagga gagcgagaag agtcacaaca aaattcgcca tcgctcggtt
    12541 ttcgcggaga aggaatgaga gacgaagaga accgtctgag ttcagttcag aaagaatctc
    12601 tgaaatcttg cgttcaattt gttcctcttc cggtagggac gacggaacgc cgctccaatc
    12661 gtcctcattc tcgaaaatgt atggcgctgc attttttgtc cgtcgtttta aatctctcaa
    12721 gctccaccaa agacggcata gagcaaacag gtcgccaagg tcaaaagacg gttcatcgtc
    12781 ctcttcctcg ggggcgctgc ggaagaaaaa acggtcgcgt tcctctttga gaattgcaag
    12841 atcccgtgca gccgaccgat agggacgata cctcgatagt ttttcgagga catcttcacc
    12901 gggatcgttc tcgggttctt cgctttcgta cagtgattcg cgtcccggca gaagggcgcg
    12961 aatcttgttg agaacaagag aagctgccat aaacaaaaac tccgaaacta cggaaacgga
    13021 gaccttgttc gtgttggcca ggtaggcgcc gtaaatgcgg acgacctgac cgacggagat
    13081 actggcggct tccagctctc tgctttcaac gagccagcag agtaaatcaa agggaccgga
    13141 aaaaccgtcg atctccgcct ggaactctgc gagttctccc aggcgctcct ccactcctag
    13201 cctctcttga aaaagccgat tgcgttgatc acttcgtcaa tcgtacggcg ggcaacctct
    13261 ccggcttttt ccgcaccctc gcgaagaata tcggaaagta gatcgggcga cttctcataa
    13321 aacgtccgcc gttcctgaat cggtcccata actctttcaa cgtgcgcctg cagcttcttc
    13381 ttgcagtcca cgcagccaat tccggctgac ctgcatccgg aagcaatctc atttttttcg
    13441 tcttcgttgt tgttgaagac tttatggata tcccacacgg ggcacttctc aggatcaccg
    13501 gggtcggtgc gccgttctct cgcaggatc
//
LOCUS       DAOPKU010000011        13196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_4950_length_13196_cov_4.771022, whole genome shotgun sequence.
ACCESSION   DAOPKU010000011 DAOPKU010000000
VERSION     DAOPKU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4950_length_13196_cov_4.771022"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PK849_00745"
     CDS             <1..591
                     /locus_tag="PK849_00745"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPE64677.1"
                     /translation="YSCGKQMMDRVIVAYGQEKGLRYTLFRPFNWIGPRLDTFKDAEE
                     RTARSITQMVYDVLSGRGITLVNGGEQMRSFTHISDGVAALMAILADEKASNGKIFNI
                     GNPANNASIKELAHIIINVMKEIPSFREAAEKTTVTVMPAEQYYGNGYDDMKNRVPSI
                     QAIGAALGWKPLVPLREAVAMTVASYLDSGEEQKRG"
     gene            584..1504
                     /locus_tag="PK849_00750"
     CDS             584..1504
                     /locus_tag="PK849_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPE64678.1"
                     /translation="MVDTVLVLKTDVDTLRGYREGVPRLLEIFRARKLRASFFFSFGP
                     DNSGKAIRRIFKKGFLQKMRRTKAASAYGLRTLLYGTLLPAPKIVESEPSVFIRAVEE
                     GHDCGIHSWDHVHWHDNLSKMSEEQIRGEFRKAMELFERYARRTAKSCAAPGWQVTPA
                     SLAVQDELGFDYCSDVRGSGYAFRPRMGGRTFKTLQIPTTLPTLDEVLGDVPGDRIND
                     HYMELLQPGLNVHTIHAEMEGGGQSALFEELLDRCVKRGVVFKTLDDIALEISLKKPI
                     IHTCDVVAGETPGRWGTVSVQRCAREETAD"
     gene            complement(1546..3576)
                     /locus_tag="PK849_00755"
     CDS             complement(1546..3576)
                     /locus_tag="PK849_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE64679.1"
                     /translation="MKSIRTKIMASFIAATIVFMTIMGVTLYARVDGKVTDLTEDMAL
                     QISRASSVGLSEWLDNMVNLTGAFAGMDVVRSLEWKNAEAFLEGQMKKHHRLFEMLFI
                     SGTDGRIKGTGGTTADISQRPYFKELMSGGKQYAISDPVVSLATGNVVFVIAHAVPGA
                     DGKPAGVFAATVLLQNLQGLVDAVRVGETGFGWIVDGGGIVAAHRDKEHIGKFDVRQD
                     AAYTTVWKRMSAKETGYELHKRFDGTPAYVFFAPIAGSPGWTFAVTVLEREIKATAVD
                     LSWTIVIIVAFVIIAFAIISLLIGNSIARPVQTVQRLAERAKGGDLTILRSDFNIATK
                     DEIGRMADALADMIRVQREMVHDLKEKAVTLSALSEETAASTEEVTSTVSEMAESNAT
                     LAEKTRNGRKDTVEASMVMLEMSSLIQIAQNLASRADKNSEEMTEAALQGRETVEKTI
                     TQMESVKEAVSETEAFLSQLDTFSARIGVVGETITGIADQTNLLALNAAIEAARAGEA
                     GRGFAVVAEEVRKLAEQSQKGAQEVSELVAKILEGTRSAVSSMQKSQEGVMEGVSVAH
                     DAGDALRKIKSAIESSIADVRKIIKTTDEEVAKSDKVIALINASATVMESTDDNVQSM
                     AAAMEETAAAMENVATSSQEVSETAEDLRRISERFKVDDEDGVQGGGLVPVK"
     gene            3839..4612
                     /locus_tag="PK849_00760"
     CDS             3839..4612
                     /locus_tag="PK849_00760"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HPE64680.1"
                     /translation="MRKTTNTVALALALLFFAISAFAEQPTREFKGSFEPANNGERLL
                     SLLVSLTDPESMELRMDQQPDNDGNIRDFSIFIQGASIGGFRVEKIAIESSFLELNRP
                     SEWRTGERDSLKVRNVLRSNVELVALEKDINAALTSYAGGDWRRISVDLKRGMVSARG
                     NYRSGSLDILAEVSTKLEIRQGKQIWLKDTAIKINHDDQTDVIQKELAKIQPVVDIGQ
                     FPFPVTLAVLKVDEQKLVFSTRTLPKPVKGIFYRYVRGS"
     gene            complement(4594..5778)
                     /gene="truD"
                     /locus_tag="PK849_00765"
     CDS             complement(4594..5778)
                     /gene="truD"
                     /locus_tag="PK849_00765"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HPE64681.1"
                     /translation="MRIKTAPDDFRVEEVLHFAPAKKEAPFRVFLLEKEGWNTADAIQ
                     EAAKACGVPLAEIRSVGKKDRHAHTLQHVSVPLKYDLAYEGKGFVIRFAGYSTAHLAP
                     SVLKGNRFSAALRDMTAAEAGACEARLERIAARGFANYFDDQRFGNVGEKGEFFAEML
                     VKRRFGPALRKYFSFRHPEAPLPLKKRKAQLAALWGKWEEMVPLCPEPLLKQMLDILR
                     RKGESGYRACLKLIPAQEMGLYYSAYQSFLWNETLCRFLSKQPGELFPVPVKTGELLQ
                     YRMLDESLLSRLLTEPMHTAAPDMPQLPSDLGDVFSAVLFERGVRLSKFRLKEVEQAY
                     FSSFPRACAVIPENMTWKREEDALFPGKTALRVDFFLPRGSYATMMLKSAEEGGVQEP
                     RT"
     gene            complement(5803..7176)
                     /locus_tag="PK849_00770"
     CDS             complement(5803..7176)
                     /locus_tag="PK849_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE64682.1"
                     /translation="MYDVIIVGSGPAGIFAAMECVRHEKKVLIIDKGRLIRERKCPIV
                     EGTSKTCLNCSSCSIVSGWGGAGSASDGKLTLTTGFGGNLEESIGEDALLDMIAQVDK
                     VFVEYGADNHAYEPSGDIVRDTIRKASSVGIKILPARIRHIGTDASREVLNNMYEALK
                     PKCDIMMGTFVEDLLIENGRACGVVLADGTVHRSASVIVAPGREGASWLAGVVAKLNI
                     PIASMPVDIGVRVEVPDSVCEDLTRHFYEIKCLYNTPTFDDRCRTFCMNPSGFVVYEY
                     NQAHDLVTVNGHSLKNVKSRNTNFAILVTKNFTQPFNDPIGYATHVAKLANMLAGGGI
                     LVQRLGDLRDGRRSTQSRIDRGMITPTANAQPGDLSLVLPHRYLTDVVEFLDGLNSIM
                     PGVNQNDTLLYGVEIKLYSLRLELRKNLEVPVIERLFMAGDGAGVSRGIIQAAASGVV
                     AARGALA"
     gene            complement(7273..7635)
                     /locus_tag="PK849_00775"
     CDS             complement(7273..7635)
                     /locus_tag="PK849_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64683.1"
                     /translation="MALRVCEYCGKAWFDEGGEKQVALCPPCAQEVQGIYMLAWGYLR
                     DNTAEAGRHRKISIQQLADELGIDTKAINLLVKMGKIQVESFAGEGNADEIARNLEIL
                     RKLRKNVRGKSKYDRSGR"
     gene            complement(7640..8293)
                     /locus_tag="PK849_00780"
     CDS             complement(7640..8293)
                     /locus_tag="PK849_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPE64684.1"
                     /translation="MGRVQIIVEGMTASGKSTVVNLLSERLGLRVMPEEFRDQYDLLR
                     RFHLERRWAFPMQLNFLVTRFAQYLCASESDEYILDRSVFGDKIYATLYYKLGYFRDS
                     QFGTYLMLYDSLLKHLAMPKLIIVLSCPFEEIMRRIRMRGRSDEIEAGEEYWRSLYDA
                     YASFLDFVRSEIGLSATRCITLDLGDPSFIHTPERVERFIEEVTEALDMEAPVTTGG"
     gene            complement(8355..11036)
                     /locus_tag="PK849_00785"
     CDS             complement(8355..11036)
                     /locus_tag="PK849_00785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE64685.1"
                     /translation="MKDSLGVVRERNVGEIGGRQAGTCPEGYCEKTLRLEKELTRWKR
                     EEAVLRDIERRYLALLDSPLLILLILSEGRVLFMNRRGEEFFGFALRERPRFLLSEYA
                     ASGYASSVESLFTPGSDGAVRGGRLDFAVRSESGRERRIDCAFVPGVYQGAPVLLGAG
                     YELPEGGEEGSETDSVDVLLSEREDLLFCSMDTEYVPTFMTEGFRNASVRIWGRSPDQ
                     NEALLKILPSGEHGESFRLAAERAHAGEYAETAFEAGELVYSCSFAPLYSKEGEPLGI
                     SLLLTDRTELCRLERERYAEAESFQRLFASSGEMMLIASVEDGKLLRGSPAFLSWMGY
                     EETAILGRTESDLALYADALQRERLVNELEENGSVEDFEVEMRTASGDLVPGRASVSR
                     ILHAGRDSLLYVFHETVAEPEEEEPVATSEDADVVEEQESMSEAIQEIPGAEEKESVA
                     AFPVDGFDGFELPEPPEGEPQTLIDSSLLPKEGVQDARLRSMLAQNEDTMLCILDEEC
                     VPIIMTKGFEHVCETLWGRLPAEGEALHNLLPAGVQGEAFRVAVERARNGERSKTGQE
                     AGDRYFSLSFSPVDDSEGRFSAISLAATDLTESRARERECRAHADNFRRLFSSSPEML
                     LISSVKEGRVLSCNRAFLSRMGFAETSVLGRTEEELGIYNGAGQRERLVKELDDAASV
                     ENYEVQLKSSSGDVFPGRVSVERIESEGAAALLSLFRDITNEKQAEECKQAATDMLSG
                     IPSRSGFERILNTEIDRAMRYRGNMSLILIDVDGFGALNETLGREAGDRVLKDFCAAV
                     KSRIRPTDFIGRWGGDEFAVLTPMSGRIAFQIAEAIRDMIYNYRFLPDRILSSSMGVA
                     EFRRSMDMNEFVKRAEDALAEAKKAGGNKAILAPFVP"
     gene            complement(11082..11600)
                     /locus_tag="PK849_00790"
     CDS             complement(11082..11600)
                     /locus_tag="PK849_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HPE64686.1"
                     /translation="MDYVGLLLIFCARIVDVSCGTVRILFLVRGRRALAAMIGFVEVM
                     IYLTALGHIIGGGREMNWIQMVVYCAGFSTGNFIGSLLEEKLMNAYVFLELIMDRTAE
                     TKEMIDNIRADGFGATVIYGRGRDGVRTIVKIICRRRDVPVIVAHTHNKGFICISDVK
                     GCSGGQFRFQRK"
     gene            complement(11600..11731)
                     /locus_tag="PK849_00795"
     CDS             complement(11600..11731)
                     /locus_tag="PK849_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64687.1"
                     /translation="MSEVLRRKGRVLSNASLRPLFEKTGAGKALLQALRATKYGGGI"
     gene            11893..13125
                     /locus_tag="PK849_00800"
     CDS             11893..13125
                     /locus_tag="PK849_00800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE64688.1"
                     /translation="MNGEQTIPELEERIVFLTMERRAAMEALEVALDISSFTASLTGI
                     ESVEEILRESAVKIRALLPFEALAFFLADEDDDMALAPAYADPPEKMHVIEQEKKPLI
                     DDRTVAWALRRKKPVIVATQDRSGRIFLHALASPSRVLGLFIGILGKEEEAQQTPSYP
                     DFLSIVLSATSGVIGTNMLLRKINLLNLALQEKVHFLEKSKEELSLYRNNLEREVAAR
                     TKELARRNEDLRREIVERKRMESEIRYRAWHDALTGLANRELFHSRLLEAVAKGEPIA
                     VFFMDLDGFKNINDTLGHDSGDSLLQEAARRIAREMGEENTVARMGGDEFTVIISFPT
                     SRELLAECAERLLHGISEPISLEGASVSITASIGISRFPEDGATTQDLMKCADVAMYK
                     AKERGKNRYCFWSDIRIK"
BASE COUNT         2754 a         4167 c         3627 g         2648 t
ORIGIN      
        1 tacagctgcg gcaagcagat gatggacagg gtcatcgtgg cctacgggca ggagaagggg
       61 ctccgctaca cgctcttccg cccgttcaac tggatcggcc cccgcctcga caccttcaag
      121 gacgccgagg agcgcaccgc ccgctccatc acgcagatgg tctacgacgt gctctccgga
      181 cgggggatca cccttgtcaa cggcggagag cagatgcgga gcttcacgca catctccgac
      241 ggcgtcgcgg cgctgatggc catactcgcc gacgaaaaag cgagcaacgg gaagatcttc
      301 aacatcggca acccggcgaa caacgcatcc atcaaggagt tggcccacat catcatcaac
      361 gtgatgaaag agatcccctc tttccgggag gccgcggaaa agaccaccgt gacggtgatg
      421 cccgccgaac agtattacgg taacggctac gacgacatga agaaccgcgt tccttcgatt
      481 caggccatcg gcgccgcgct cggctggaag ccgctcgtgc cgctccgcga ggccgtggcg
      541 atgactgtgg cgagctacct tgattcagga gaggagcaga agcgtggttg atacggtgct
      601 cgtcctcaag acggacgtcg atacgctgag aggctaccgc gagggcgtcc cccgtctcct
      661 ggagatcttc cgcgccagga agctgcgcgc ctccttcttc ttctccttcg gtccggataa
      721 ctccggcaag gcgatccggc gtatcttcaa aaaagggttc ctgcagaaga tgcggcgtac
      781 caaggccgcc tcggcgtacg gactccgcac cctgctctac ggcacgctgc ttccggcgcc
      841 gaaaatagtg gagagcgagc cgagcgtctt catccgggct gtcgaggagg gacacgactg
      901 cggcattcac agctgggacc acgtccactg gcacgacaac ctttcaaaaa tgagcgagga
      961 gcagatacgc ggggagtttc gaaaggcgat ggagctcttc gagcggtacg caaggagaac
     1021 ggcgaaaagc tgcgccgcgc ccggctggca ggtcacgccc gcgagcctcg ccgtgcagga
     1081 cgaactgggc ttcgattatt gcagcgacgt ccgcgggagc ggatacgcct tccgtccgcg
     1141 catgggaggg cgtaccttca agacgctcca aatccccaca acgcttccca ctctcgacga
     1201 agtgctcggc gacgttccgg gggacaggat caacgatcat tacatggagc ttcttcagcc
     1261 cgggctgaac gtgcacacca tccacgcgga gatggagggg ggcggacaga gcgcgctctt
     1321 cgaggagctg ctggaccgct gcgtgaagag aggcgtcgtc ttcaagacgc tcgacgacat
     1381 cgccctggag atttcattga agaaaccgat catccacacg tgcgacgtcg tcgccggaga
     1441 aactcccggc cgctggggga cggtttccgt ccaacggtgc gcccgcgaag aaacggcgga
     1501 ctaaaaaaga gggggccagc aagccccctc ttctgtactc cgtatttatt tgactggaac
     1561 gagccctccg ccctgaacac cgtcttcgtc atccactttg aaacgctccg agatccggcg
     1621 cagatcctct gcggtctcgc tgacctcctg cgagctggtc gcgacgttct ccatagccgc
     1681 ggcggtctcc tccatggcgg ccgccatgct ctgcacgttg tcgtccgtgc tctccatcac
     1741 cgtagcgctg gcgttgatca aggcgatgac cttgtcggac ttggcgacct cctcgtcggt
     1801 cgtcttaatg atcttacgaa catccgcgat ggagctctcg atcgcacttt ttatttttcg
     1861 aagcgcatcg cctgcgtcgt gcgcgactga aacgccctcc ataacccctt cctggctctt
     1921 ctgcatggag gagacggcgg agcgtgttcc ctcaaggatt ttggccacga gttcggagac
     1981 ctcctgcgcc cccttctggg actgctcggc aagcttccgc acctcttcgg ccacaacggc
     2041 gaaaccgcgt ccggcctcgc ctgcccgggc ggcctcgatc gccgcgttca gggcgagcag
     2101 gttcgtctgg tcggcgattc cggtgatcgt ctcgccgacg acgccgatac gcgccgaaaa
     2161 cgtatccagc tgcgaaagaa acgcttccgt ctcggagacc gcctccttca ccgattccat
     2221 ctgcgtaatg gttttttcga cggtctccct gccctggagc gcagcctcgg tcatctcctc
     2281 cgagttctta tccgcccttg acgcaaggtt ttgcgctatc tgtatcagac tgctcatttc
     2341 gagcatcacc atcgaggctt cgacagtgtc ttttcgtccg ttcctcgtct tctccgcaag
     2401 cgtcgcgtta ctctccgcca tctcgctgac cgtgctcgtc acctcttcgg tcgaggcggc
     2461 cgtctcctcg gagagcgcgg aaagggtcac cgccttctcc ttcaaatcgt gcaccatctc
     2521 gcgctggacg cggatcatat ccgcgagcgc gtccgccatt cgcccgattt catccttcgt
     2581 cgcaatgttg aagtcgctcc tgagaatcgt gagatcgccg cccttcgccc tctcggcaag
     2641 acgctggaca gtctgcacgg gccgagcgat cgagttgcct atgaggagag agatgatcgc
     2701 gaaggcgata atgacgaagg cgacgatgat cacaatcgtc cacgaaagat cgactgccgt
     2761 cgccttgatc tcccgttcca ggacggtgac tgcgaacgtc cagccgggcg atcccgcgat
     2821 aggggcgaag aaaacgtatg ccggagtccc gtcgaatcgt ttgtgcagct cgtaaccggt
     2881 ctcttttgcg gacatcctct tccataccgt cgtatacgcc gcatcctgac gcacgtcgaa
     2941 cttcccgata tgctccttgt cgcgatgcgc ggcgacgatg ccgccgccgt cgacgatcca
     3001 tccgaacccg gtctcgccca cccggacggc gtcgacaaga ccctgcaggt tctgcagaag
     3061 caccgtcgcg gcgaagactc cggccggttt gccgtcggcg cccggcacgg cgtgcgcaat
     3121 gacgaagacg acgtttcctg ttgccagaga gacgaccggg tcggaaatcg cgtactgctt
     3181 cccgccgctc atgagctctt tgaaatacgg tctctgtgag atgtccgcag tcgtcccccc
     3241 cgttcccttg attcgtccgt cggtaccgga gatgaagagc atttcgaaaa gacggtggtg
     3301 ctttttcatc tggccttcaa gaaaggcttc ggcattcttc cactcgagcg aacgaaccac
     3361 gtccatcccc gcgaacgctc ccgtcagatt caccatgttg tccagccact ccgacagacc
     3421 gacggagctc gccctcgaaa tctgcagggc catatcttcg gtaaggtcgg tcaccttgcc
     3481 gtcgacgcga gcgtagagcg tcactcccat aatcgtcata aacacaatgg tcgctgcaat
     3541 aaatgatgcc atgatcttcg tccgtatgct cttcaacgcc catccaccat cctttcagcc
     3601 gtctcttaca acgccgtctc cttcaccgca ggattctcga aggcgttttt gcacgcctgt
     3661 atcgctcaca acctcttccc gcatttcaaa atagatatag agcgcacgct gattttctgc
     3721 tagtataacc taagaaacgc aagaggaata gtctcggaaa agagaggagg tttatttccc
     3781 ggcgccattc cgaaggcaat aaatagatag agcattctac attcaaggag gaaaaatcat
     3841 gagaaaaaca accaataccg ttgcgcttgc gctcgcactg ctcttctttg cgatctccgc
     3901 cttcgcggag cagccaacca gggagttcaa gggaagcttc gaacctgcga acaacggaga
     3961 acgcctgctc tccttgctgg tttcgctgac cgaccctgaa tcaatggagc ttcgcatgga
     4021 ccagcagccg gacaacgacg ggaatatacg cgacttttcc atcttcatcc agggggcttc
     4081 catcgggggg ttccgggtgg agaagatcgc gatcgagtcg tccttcctgg agctcaaccg
     4141 accgtcggag tggcggacgg gggagcggga ttcgctcaag gtcagaaacg tccttcgcag
     4201 caatgtggaa ctcgtcgcgc ttgaaaagga tatcaacgcc gccctgacct cctacgccgg
     4261 aggcgactgg aggcgcatct ccgtcgacct gaagagggga atggtcagcg cgcgcgggaa
     4321 ctaccgctcg ggctccctcg acatcctggc ggaggtgtcc acaaagctgg aaatccggca
     4381 ggggaagcag atctggctga aggataccgc gatcaagatc aaccacgacg accagacgga
     4441 cgtcatccag aaagagctgg cgaagattca gcccgtcgtc gatatcgggc agttcccctt
     4501 cccggtgacg cttgccgtgc tcaaagtgga cgaacagaag ctcgtcttct cgacgagaac
     4561 gcttcccaag ccggtcaaag ggatcttcta ccgctacgtc cggggctcct gaacccctcc
     4621 ctcttcggcg ctcttgagca tcatcgtcgc ataacttcct cgcgggagga agaagtcgac
     4681 gcgaagcgcg gtttttcccg ggaagagggc atcctcttcc cttttccatg tcatgttctc
     4741 cggaatcacg gcgcaggcgc gcgggaagga ggagaagtac gcctgctcga cctctttcag
     4801 ccggaatttc gagagacgga ctccccgttc gaaaagcacc gcggagaaaa catcgccgag
     4861 gtcggaggga agctgcggca tatccggcgc cgcagtgtgc atcggctcgg taagcaggcg
     4921 cgaaagaagc gattcatcaa gcatgcggta ctgaaggagt tcgccggttt tcaccggcac
     4981 aggaaagagc tcgcccggct gcttcgaaag gaagcggcag agcgtctcgt tccacaaaaa
     5041 gctctgatag gccgagtagt agagccccat ctcctgcgcg ggtatgagct tgagacaggc
     5101 tctgtacccg ctctccccct tgcggcggag gatgtcgagc atctgcttca acagaggttc
     5161 gggacagagc gggaccatct cctcccactt tccccagaga gcggcgagct gtgcctttct
     5221 ctttttgagc ggcaggggcg cttcagggtg ccggaaggag aagtacttcc tgagggcggg
     5281 tccgaagcgc cgttttacca gcatctcggc gaaaaactcc cccttctctc ccacgttgcc
     5341 gaagcgttgg tcgtcgaagt agttggcgaa gccgcgcgct gcgatccgct ccagccgcgc
     5401 ctcgcacgcc ccggcctcgg cggctgtcat gtcgcggagc gcagcggaga agcggttgcc
     5461 tttcagcacg gacggcgcca ggtgcgccgt ggagtatccc gcaaagcgga tgacgaaccc
     5521 ctttccctca tacgcaagat cgtacttcaa cggcacggaa acgtgctgga gcgtgtgcgc
     5581 gtgccggtct ttcttcccta ccgagcggat ctcggcgagc gggacgccgc aggccttcgc
     5641 cgcttcctga attgcatcgg cggtgttcca cccctccttc tcaagcagga agacgcggaa
     5701 cggcgcctcc ttcttcgccg gagcaaagtg aagcacctct tccacacgaa aatcgtcggg
     5761 agctgtcttg attcgcatct gtcttctcct ctgctgcggg cgctaagcca gcgcgccgcg
     5821 cgcggcgacg acgccgctcg ccgcggcctg tatgattccc cggctgacgc ccgcgccgtc
     5881 gccggccatg aagagacgtt cgatgaccgg cacctccagg ttcttccgaa gctccagccg
     5941 gagcgagtac agcttgatct ccaccccgta gagcagcgtg tcgttctggt tcacgccggg
     6001 catgatggag ttgaggccgt cgagaaattc cacaacgtcg gtcagatagc ggtgcggcag
     6061 cacaaggctg aggtcgccgg gctgcgcgtt tgccgtcggg gtgatcatcc cgcggtcgat
     6121 gcgcgactgg gtggaacgcc gtccgtcccg gagatcgccg agccgctgga cgagaattcc
     6181 gcctccggca agcatgttcg ctagttttgc gacgtgcgtg gcgtacccta tcgggtcgtt
     6241 gaacggctgc gtgaagttct tcgtcaccaa aatggcgaag ttcgtgttcc tggacttcac
     6301 gttcttcagg ctgtggccgt tcacggtgac gaggtcgtgc gcctggttgt attcgtagac
     6361 gacgaatccc gacggattca tgcagaaggt gcggcagcgg tcgtcgaagg tcggcgtgtt
     6421 gtacagacac ttgatctcgt agaagtgccg tgtgaggtct tcgcagacgc tgtccggaac
     6481 ttccacccgc acgccgatat ccaccggcat agaggctatc gggatgttca gtttcgcgac
     6541 aactccggcc agccaggaag ccccctccct tccaggggcg acgatgacgc ttgcgctcct
     6601 gtggacggtt ccgtcggcaa ggacgacgcc gcaggcgcgt ccgttctcta tcaggagatc
     6661 ctcgacgaac gttcccatca taatgtcgca cttcggcttc agcgcctcgt acatattgtt
     6721 gagcacttcg cgcgaggcgt ccgtgccgat atggcggatc ctcgcgggca gaatcttgat
     6781 cccgacgctg gaggccttcc tgatggtatc gcggacgatg tcgcccgagg gttcgtacgc
     6841 atggttgtcg gcgccgtact cgacgaacac cttgtccact tgcgcaatca tgtcgaggag
     6901 cgcgtcctcg ccgatgctct cctcaagatt accgccgaaa ccggtggtca gcgtcaactt
     6961 tccgtcggat gccgaacccg cgccgcccca ccccgagacg atgctgcacg aggagcagtt
     7021 gaggcaggtc ttgctcgtcc cctcgacaat cgggcacttg cgctcgcgga tcaagcgccc
     7081 cttgtcgatg atcagcacct ttttttcatg ccgcacgcac tccatggcgg cgaaaattcc
     7141 ggcaggaccg gaacctacta taatgacgtc gtacattgtg agacgcactt cctttcatag
     7201 cgtcctcaaa aaagcgccgg agttcctcgc ctgccgtcgg acggcaagcg gcttcaaaaa
     7261 gagacgaagg cgttaccgcc cgcttctgtc gtatttcgac tttcctctga catttttccg
     7321 gagcttccgg agtatttcga gattcctcgc aatctcgtcg gcgttccctt cgccggcgaa
     7381 cgactcaacc tgaatcttcc ccatcttcac aagcaggttg atcgccttcg tatctatccc
     7441 cagttcatcg gcaagctgct gtatcgagat tttccggtgt ctgcccgcct ccgcagtgtt
     7501 gtcccgcaga tagccccatg cgagcatgta aataccctgc acctcctgag cacacggagg
     7561 acagagcgct acctgcttct ccccgccctc gtcgaaccat gccttgccgc aatactcgca
     7621 tactctcaac gccatagcgt cagcctcccg tcgttaccgg cgcctccata tccaatgctt
     7681 ccgtcacttc ctcgatgaaa cgctccactc tttcgggagt gtgaatgaac gaaggatcgc
     7741 ccaaatccag ggttatgcag cgcgtcgccg agagtcctat ctccgagcgc acgaaatcga
     7801 ggaaagacgc atacgcgtcg tagagcgaac gccagtactc ctcacccgcc tctatctcgt
     7861 cggagcgccc ccgcatacgg atgcgccgca tgatctcctc gaacggacag gagagcacaa
     7921 tgatcagctt gggcatggcc agatgcttca gaagcgaatc gtacagcatc aggtacgttc
     7981 cgaactggct gtcccggaaa tagcccagct tgtagtagag cgtcgcgtag atcttgtcgc
     8041 cgaagacgct gcggtcgaga atatactcgt cggattccga cgcgcagaga tactgggcga
     8101 acctcgtcac gaggaagttg agctgcatag ggaaagccca gcgccgctcc aggtgaaaac
     8161 ggcgcagcag atcgtactga tcccgaaact cctcgggcat gacacgcagt ccaagccgct
     8221 cggagaggag attcaccacc gtcgatttgc cgctcgccgt cataccctca acaataatct
     8281 gcaccctgcc catattcttc ctcctccggt tgtatcatcc ccgacgtttc cggcgcgaaa
     8341 agggcgacgc tacgtcaggg gacgaagggc gcgaggatcg ctttgtttcc ccccgccttt
     8401 tttgcctcgg cgagcgcgtc ctccgctctt tttacgaatt cattcatgtc catgctccga
     8461 cgaaactcgg cgacccccat gctggaggag agtattctat cgggaaggaa tctgtagttg
     8521 tagatcatgt cgcgtatcgc ctccgcaatc tggaaggcga tacgtccgct catcggcgtc
     8581 agtacggcga attcgtcccc gccccagcgt ccgatgaaat cagtgggacg gatgcggctc
     8641 ttgacggcgg cgcagaagtc cttgaggact ctgtcgccgg cttcccgccc gagtgtttcg
     8701 ttcagcgcgc cgaagccgtc cacgtctatc agaatgagcg acatgttccc tctgtacctc
     8761 atcgcccgat cgatctccgt attcagaatc cgttcgaacc cgctgcggct gggaatcccg
     8821 gagagcatat ccgtggcggc ctgtttacac tcttcagcct gtttttcatt cgtaatatcc
     8881 cggaaaagag aaagaagagc cgccgcccct tccgattcga tcctctcgac ggagacacgc
     8941 ccgggaaaca catcgccgga ggacgacttc agctgcacct cgtaattttc gacagacgcc
     9001 gcgtcgtcca attctttcac cagccgctct ctctgccccg ctccgttgta gatgcccagc
     9061 tcctcctcgg tacgccccaa caccgaggtt tcggcgaatc ccatccggga gaggaacgca
     9121 cggttgcagc tgagcacacg cccctccttc accgaggaga tcagcagcat ctctggagag
     9181 gaggagaaca agcgccggaa gttgtccgca tgggcgcggc actcccgctc ccgggcacgg
     9241 gattcggtca ggtcggtcgc tgcgagcgaa atggctgaaa aacggccttc cgagtcatct
     9301 accggtgaaa aggagagcga gaagtaacgg tcgcccgcct cctgccccgt cttcgaccgc
     9361 tccccgttcc gggctcgctc tacggcaaca cggaacgctt ccccctgcac acccgcgggc
     9421 agaagattat ggagggcttc tccttcagca ggaagacgtc cccagagggt ttcacaaaca
     9481 tgttcaaacc ccttggtcat gataattgga acgcactctt cgtccagaat gcagagcatc
     9541 gtatcctcgt tttgtgcaag catggagcgc agccgcgcgt cctgcacacc ctccttcgga
     9601 aggagagagg aatcgataag cgtttgcggt tccccctcag gaggctccgg gagttcaaag
     9661 ccgtcgaaac cgtccactgg gaatgcggcg acgctttcct tttcttcagc tcccgggatt
     9721 tcttgaattg cttcggacat gctctcctgt tcttcgacga catccgcatc ttcgctcgtc
     9781 gcgacaggct cttcctcttc cggttcggca actgtttcgt ggaagacata aaggaggctg
     9841 tcgcgccccg cgtgcagaat cctcgatacg gaggctctgc cgggaacaag atcgccggac
     9901 gccgtccgca tctcaacctc aaaatcctct acagagccgt tctcttccag ttcatttaca
     9961 aggcgctccc gttgcagggc atctgcatac agagcaagat ccgactccgt gcgccccaag
    10021 atcgccgtct cctcataccc catccatgag agaaacgcgg gagatccccg aagcaacttc
    10081 ccgtcctcca cggaggcgat cagcatcatc tccccggagg aggcgaaaag acgttgaaag
    10141 ctctccgcct cggcgtaccg ctccctctcc agccggcaga gttctgttct gtcggtcaga
    10201 agcaacgaaa ttccgagcgg ctccccttcc ttgctgtaga gcggagcgaa ggaacaggaa
    10261 tacacgagtt ctccagcctc aaaagcggtt tcagcatact ctccggcatg cgctctctcg
    10321 gccgcgaggc ggaacgattc tccatgctca ccggagggga gaatcttcaa aagcgcttca
    10381 ttctggtcgg gactcctccc ccagatacga acggaagcgt ttctgaaacc ctccgtcata
    10441 aacgtcggaa catactccgt atccatggaa cagaaaagaa gatcttctct ttccgagagc
    10501 agcacgtcga cagaatcggt ctccgagcct tcctccccgc cctccgggag ttcatacccc
    10561 gcccccagga gcaccggcgc tccctggtac acgccgggga caaacgcgca gtcgattctc
    10621 cgctcccgtc cgctctccga acgaacggcg aagtcgagac gccctccgcg aacggcgccg
    10681 tcggaaccgg gagtgaagag gctctctacc gatgaagcat atccggacgc tgcatactcc
    10741 gaaaggagaa atcgggggcg ttcgcgaagc gcaaatccga aaaactcctc gccgcgcctg
    10801 ttcataaaga gcactctccc ctcggagagg atcagcagga tcaggagcgg gctgtcaagc
    10861 agcgcaagat agcgccgttc aatgtcgcgc agaacggcct cctcgcgttt ccagcgggtc
    10921 aactcttttt ccagccgaag ggtcttttcg cagtacccct ccggacatgt tcccgcttga
    10981 cgccccccga tctctccgac gttcctctcg cgaacaactc ccagactgtc cttcatgaag
    11041 ccccatccct tccttccgtg aaccaggggt atgaaccaaa gttacttgcg ttgaaaacgg
    11101 aactgcccgc cggagcaccc cttgacgtcg cttatgcaga tgaacccttt attatgtgtg
    11161 tgcgcgacga tgacgggtac gtcccttctg cggcagatta tcttcacgat ggtgcggacg
    11221 ccgtccctac ctcggccgta gatcaccgtc gccccgaaac cgtccgcacg gatattgtcg
    11281 atcatctctt tcgtctcggc cgttcggtcc attatgagtt ccaggaagac ataggcgttc
    11341 atcagttttt cttccagcag ggagccgatg aaatttcccg tggaaaaacc ggcgcagtag
    11401 accaccatct ggatccagtt catttctcgt ccgcccccga tgatgtgccc aagggcggta
    11461 agatagatca tcacttcgac aaagccgatc atcgccgcca gcgctcttct ccctctgacg
    11521 aggaagagaa tccgcaccgt tccgcagctt acgtcgacaa tacgggcgca gaagataaga
    11581 agaagtccga cataatccat cagatccctc ctccgtactt ggtcgcccgg agcgcctgca
    11641 gaagagcttt cccggcgccg gtcttttcaa agagaggacg aagagaggcg tttgaaagaa
    11701 cgcgtccctt ccttcgcaac acttcagaca ttccgaaacc tccttgcttc ccggtaaaga
    11761 cactcagtgc atgccgggac gagcaatttt tgatacaaca taccgttact atcctatcat
    11821 acctctccgg cagagtataa taaacggcaa atgagcaaaa acgcaattct ttcgcgtaaa
    11881 aggagagacg ctatgaacgg cgaacagacc atccccgagc tcgaagaacg gatcgttttt
    11941 ctcaccatgg agcgtcgcgc cgcaatggag gcgcttgagg tcgccctgga catcagctcc
    12001 ttcaccgcgt cgcttaccgg catagagtcc gtggaagaga tcctccggga gtccgcggta
    12061 aaaatacgcg cgctcctccc gttcgaggcg ctggcattct tcctcgccga cgaggacgac
    12121 gacatggcgc tcgctcccgc gtacgccgat ccgccggaaa aaatgcacgt catcgagcag
    12181 gaaaaaaaac cgcttatcga cgaccgcacc gtcgcgtggg cgcttcgcag aaagaaaccg
    12241 gtcatcgttg caacgcaaga ccgctccggc agaattttcc ttcatgcgct cgcctcgccc
    12301 tcgcgagtac tcggcctctt tatcgggatt ctcgggaagg aagaagaagc acaacaaacg
    12361 ccttcctacc ccgactttct ctccatcgtc ctgtcggcaa catccggcgt gatcggcacg
    12421 aacatgcttc ttcgaaaaat caatctgctc aatctcgcgc tccaggaaaa agttcacttt
    12481 ctggaaaagt cgaaggaaga gctgtcgctc tatcgcaaca acctggaaag agaagtagcc
    12541 gcacgaacaa aggaactcgc cagaagaaac gaagaccttc gccgggaaat tgtcgaaagg
    12601 aaacgaatgg aatcggagat ccgctatcgc gcgtggcacg acgcgcttac aggccttgcg
    12661 aacagagagc tctttcactc ccgcctgctc gaagcggtcg caaagggaga accgattgcc
    12721 gtcttcttta tggatctcga cggcttcaag aacatcaacg acaccctcgg acacgacagc
    12781 ggcgatagcc tgctgcaaga agctgccagg aggattgccc gggaaatggg agaagagaat
    12841 accgttgcac ggatgggcgg agatgaattc acagtgatca tctcctttcc cacatcgcgg
    12901 gaactgctcg ccgagtgcgc cgaaaggctg ctgcatggca tatcggaacc gatcagcctc
    12961 gaaggggcct ccgtctccat cactgcgagc atcggcatca gccgcttccc ggaggacggc
    13021 gccaccacgc aggacttgat gaaatgcgca gacgtagcca tgtacaaagc caaagagcgc
    13081 ggaaaaaaca ggtactgctt ctggtccgat atcaggataa aatgaccgga aggtgagcga
    13141 aaaaaagggc gggaaccgat gctgcacggc cccctgccct tattctccaa atctac
//
LOCUS       DAOPKU010000012        13053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_5010_length_13053_cov_5.259578, whole genome shotgun sequence.
ACCESSION   DAOPKU010000012 DAOPKU010000000
VERSION     DAOPKU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5010_length_13053_cov_5.259578"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PK849_00805"
     CDS             complement(<1..648)
                     /locus_tag="PK849_00805"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPE64689.1"
                     /translation="MKKWILVLTALALVFAALPAAADTVVLRLGETHVADYPTTKGNY
                     EFARLVEERTGGRIKIEVYHSSQLGQEKAVIEGVQFGAIDFTRVSISPLSAFAPAFDA
                     LQMPYLYRGEEHMWKVLNGSIGEEFLASLEPANFVGLAWYDSGARNFYNSKKEIKSVA
                     DLKGMKIRVQESKLMMGLVSALGAVPTPMPFGEVYSALQTGVIDGAENNWPSYFST"
     gene            complement(765..2180)
                     /gene="uxaC"
                     /locus_tag="PK849_00810"
     CDS             complement(765..2180)
                     /gene="uxaC"
                     /locus_tag="PK849_00810"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HPE64690.1"
                     /translation="MAKKFLDKDFLLHSKAAQKLYHEYAAPMPIFDYHCHIPAEEIAG
                     DTQFASLAEAWLGGDHYKWRAMRACGVDERFVTGDASPEEKFAAWAKVVPQTIGNPLY
                     HWTHLELQRIFGVETLLNADTAAEIHDHCSKMLRKKEFSVRGLIRKFNVAALCTTDDP
                     ADSLEHHEAIKKSGFETLVAPAFRPDRALDAHLPDQFNPWLDSLAELTDVKVDSFGRF
                     IECIWRRHQYFHGKGCRLSDYGIETPYSVEWTTEDVEGSFAALRRGKTVTGDALARFR
                     SAVLFEMLTMDANQGWVQQLHLGAMRNNNSRAFSRLGPNTGYDSMGDFEQGRALVRLL
                     DRLERTGKLTKTIVYSLNPKDNDMIPTILGSFQDGKIPGKMQMGSAWWFADTKDGMTR
                     QLRALSSVGLLSRFVGMLTDSRSFLSYPRHEYFRRILCDLLGSDVERGELPEDYALLG
                     SVVQDVCFNNAKEYFQLNFNR"
     gene            complement(2267..3010)
                     /locus_tag="PK849_00815"
     CDS             complement(2267..3010)
                     /locus_tag="PK849_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HPE64691.1"
                     /translation="MATWVYDGSFNGFLCLLFESTRAGEPPEAIEHAGSGQPFLWPPK
                     EARTDELLAKQTGDVLRRRLSAPVFTRGYYAFLSALKEREMSVYTYYNLAWTLGKSVD
                     RLLTDPRVLPVHEASRAVLRERHRFLGLLRFTEIGGVLYAPFEPEADLLQLLAGHFST
                     RLGNEKWIIHDLGRGKAAIYAPRLWRIADFSLPEGIAPSRREEAFRDLWKEYFHSIAV
                     RSRENPRLQRQFMPKKYWKHLPEKEPPAE"
     gene            complement(3014..4249)
                     /locus_tag="PK849_00820"
     CDS             complement(3014..4249)
                     /locus_tag="PK849_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HPE64692.1"
                     /translation="MDAAGKLSILAEAAKYDVSCSSSGNGGRRGAVGNTSLGGICHSW
                     SDDGRCISLLKILFSNDCAFDCAYCVNRRSNDVPRASFTPRELADLVIGFYRRNYIEG
                     LFLSSAVVGSPDDTMIQLIRTAKILRDEYRFGGYIHAKAIPGASPRLVTALGFLADRM
                     SVNIELPSEVSLRALAPQKTKESIFRPMSLIGDSIAERKEKRLVSTRSLAPFVPAGQS
                     TQLIVGASPETDLSILSLAGGLYHRFGLKRVYYSAYVPVSANPLLPAITTTPPLLREH
                     RIYQADWLLRFYGFRPDEVLSPEQPNLDESLDPKTSWALRHLDLFPLDINRASYEELL
                     RTPGIGVLSAKRILTARKGRRLDEDDLKKLGVVMKRARYFLACKGFSLPSLDARALRR
                     LLTPPPPPAWKTRQLSLEF"
     gene            4486..7410
                     /locus_tag="PK849_00825"
     CDS             4486..7410
                     /locus_tag="PK849_00825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE64693.1"
                     /translation="MNALKAFSSLNADSPKRTGRMESIHWYVFFVLLLVGAVFLVMEL
                     LHARAVRKQEQFFSDRQYLQTTMSRLALEDRVSSVLMQERFLLEHLLLEPANGSFPPL
                     SDIQRALQSAIYNTADILGFYFAPHPLYSAAQRFSSFRSTPTGDAAREFAGSLEDSFD
                     FSTPRPDDLYHFSGSVRASSRVRMMALVEAAVSEKGFMGYFITIIDLTPFLVRYVLPM
                     AQGAGSAVFLIDREGVVVEHRDSSLVGGNAEKLSGGEGDALRVAWSRVRESFSGMEAI
                     ELFDVSSGMPQRKRLAWNSARVGENRLFVGVYAPESDINPVVSELRVQRFILGGVLAL
                     LLVMIAVVFIRKRSDFEVRRSESTFRAIFNSAASGIAILDANGVILSCNTTMENMTGR
                     PSAQLRMRTLFDLAAPSLQEEKEKVREALRSGALSARAELLFSRKGAEATSPENGSFW
                     GDVSLNRLERDSSTVQMLAVVTDISGLKRAEDLLRQNAETLEARKKELEHVAASQSAL
                     LEMFTFFSECVTIEELFQVLSEHLFPVIPFRCQTLFIKRSREAASFEVLDSGGDVLRS
                     MQTDFLEKKKGILGYVANTGKAYLMGDVSIDPYFIPHSPEVRSAVVAPVMYKGFLWGA
                     IVLDSDRKFAFGIRERDMLSIVGSYVALHLEEFFARQELDKKATQLRFLHRVIQQIAA
                     ERNNEALARKIVDVLFDELGFPGAGFYTPPPKGENAPVLVAGRSRAERETCSPECELE
                     AGAAMRSRTPVEREVNGRALVLSIPVTFEGEVFGALAARHDAGFSAADRELLEITAEH
                     MTTFWALNNLLAQRRHESLVDPLTQVWNRRYIMHRLDEESSRINRTNGRGSVILVDLG
                     DFKQINDRYGHVTGDEVLRVTSATMSENLRNYDMIGRYGGDEFLLYLPDVSREEAGAI
                     MVRMELKVAEQKIEGFEGAVILDYGIAGCPEDGINLSEVIRIADERMYRYKAERKAVK
                     SE"
     gene            7407..7850
                     /locus_tag="PK849_00830"
     CDS             7407..7850
                     /locus_tag="PK849_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPE64694.1"
                     /translation="MIVSLHHASFTVENLERSVAFYQETLGLKLEGVWERDQEYSEGV
                     TGIRGAWLKVAYFSLPNASFELVEYLGAKGKKIDTSTNNVGSAHVCLIVANFDAFVEA
                     LAERGVVFAGKTCTIPAGSNKGKKIAYIEDPDGNTLELVSSRALS"
     gene            7902..8096
                     /locus_tag="PK849_00835"
     CDS             7902..8096
                     /locus_tag="PK849_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64695.1"
                     /translation="MLGQYYLITGIGAFALILLAVITGLFGRNLRKIVPGPLVLKIHK
                     FSALTGALCALLHVLGVHGY"
     gene            8183..9187
                     /locus_tag="PK849_00840"
     CDS             8183..9187
                     /locus_tag="PK849_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE64696.1"
                     /translation="MPSQKILVVEDSLSQRMMISRHLRNWEYEVLEAADGVEAFTAFR
                     QQSPAIIITDLDMPEMNGFQLIEEIRRKEIARTYIIVLSASDDKSSVVSALALGADDY
                     LVKPYHPDELRVRLTGAERVLRLQNQELLIFAMAQMVDTRSPETGAHLDRVQYFSRRI
                     AEELAAEGDPLVTASWIAQLFSLSPLHDIGKIGIPDAILNKPGRLTPEEFVIMKEHVT
                     IGASIFSRIYERTHYAPFGFAIDIIRCHHEKWDGSGYPDGLAGESIPLCGRIVALADV
                     YDAISSKRVYKPSFPREQCWDIINRGRGSHFDPRLVDIFNRNEDYFWNIVEELRDIDD
                     "
     gene            9221..12109
                     /locus_tag="PK849_00845"
     CDS             9221..12109
                     /locus_tag="PK849_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE64697.1"
                     /translation="MYGQFSRFVGRISLRRIIRIAFVFQILIPVSFLGWMFFRTSRES
                     VEALSMRLGSEVAARIAEHLQSYMAVPLLVNSLNADALESGRVDIASPRIWQPFFTER
                     IRAFPSIAYTFIGTPRGEFFGARRFKENVELFVASPETTGGASVYYSVDPRGMTGDEI
                     ISYPGFDPRTRPWYTAGEHADTPVWSEIYRHFALDDLAITAVQALRGEDGSLVGVLGV
                     DLPLSAINSFLRELKVGAEGQIFILDNDGNIVADSTVDKPFHIRNGAFSRIAGVASPN
                     PAIRAAAERMAREEQGERTGRMFSVENSAGISEYVRTRPFRSLGLDWKIAVVIPEKEF
                     IGSVQRMARSAFLITLAALGLSLLTAFWVSRWICRPLSDLVESAKAITRGEWRAPFVL
                     KRDDEVGELSRSFALMSKQLSEAFSGLEEKVRLRTVELEQKNRELTEAKAVSESLAEE
                     AKAASRAKSSFLASMSHEIRTPMNAVLGLSDLVLSTPLSEEQREHLSLVQTSAESLLG
                     ILDDILDLAKIEAGKMSLENRPFRLRPLVEQVGALMRPRAEKNGVALGVSVAPELSDA
                     FEGDPLRVRQILMNLVGNAVKFTSEGSVEIVVTLAGGDEEQAQVAFAVSDTGPGIPQS
                     KVHLLFDDFTQLDGSSTRKHGGTGLGLAISRSLARLMGGDITVESEVGRGSVFRFSLP
                     LLRCSGEIVEEHEGTETDFSSMKGNVLLAEDNAVNTKMTVAMLKKTGLTVRHAQNGRE
                     AVEMWRSEPFDLILMDCEMPVMDGLEATRRIRSEEQDGKSIPIVALTAYAMKGDRERC
                     LEAGMTEYISKPIRSRKLYSLLARFLGTPSPSLHTDSEETPARFSAENGERERWESGF
                     AELLLSLDGDREALAEMVEAFFSEVPELRGSLRSSLEKGDSSAAARALHKLKGVLGYL
                     MGGEDISLFRVLEIDARTEGLKKDDPRLRELHDLLDRYELFLREAASR"
     gene            12237..>13053
                     /locus_tag="PK849_00850"
     CDS             12237..>13053
                     /locus_tag="PK849_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPE64698.1"
                     /translation="MNPQFARRMTRFQESDLTPILRLAANPEVISFAGGLPAPELFPV
                     EDLKKVAVKVLDANGRRALQYSATEGHPPLREKIAARTNRNLKTSLTAGNVVITTGSQ
                     QSLDILAKMYIDEGDCILCESPTYLGALSAFNSCLPAYREVATDGEGMIIADLEKALA
                     SEKRAKMIYVIPDFQNPSGKTWSLERRKAFLDTVSKYDILVVEDNPYGELRFEGTTPP
                     SLKSMDTRGQVVMLGTFSKIFCPGVRVAWVCGEPAFLESFCNLKQAADLHTSTI"
BASE COUNT         2600 a         3614 c         4019 g         2820 t
ORIGIN      
        1 ggtggagaag tagctgggcc agttgttctc cgcgccgtcg atgacgccgg tctggagcgc
       61 gctgtagacc tccccgaagg gcatgggcgt cggaacggcg ccgagagccg acacgaggcc
      121 catcatcaac ttgctctcct ggacgcggat cttcatccct ttcagatcgg cgacggattt
      181 gatttccttc ttggaattat agaagtttcg ggcgccggag tcgtaccagg cgaggccgac
      241 gaagttcgcc ggctcgaggc tggccaggaa ctcctctccg atcgaaccgt tgagtacctt
      301 ccacatgtgc tcttcgccgc ggtacagata cggcatctgg agggcgtcga aagcgggcgc
      361 aaaggcggaa agcggggaga tgctgacgcg ggtgaaatcg atcgctccga actgaactcc
      421 ttcgatgaca gccttctcct gtccgagctg actgctgtgg tagacttcga tcttgatccg
      481 tccgccggtg cgttcctcga cgagcctggc aaactcgtaa ttccctttcg tggtcggata
      541 gtcggccacg tgcgtctctc ccaagcggag cacgacggtg tcggccgccg ccgggagtgc
      601 cgcgaagaca agagcaagcg ctgtcagaac cagaatccac ttcttcattt tttctcctcc
      661 ttcatagtgt ggtgatgaat aacctgcgat tcctatttat tgtaaatgcc tcgcagggca
      721 aaagcaagcg tttttacaca atacgcgcac tcttcgacct tgtttcacct attgaaattc
      781 agctgaaagt attcctttgc attgttgaag cagacatctt gaactacaga gccgagaagc
      841 gcgtagtcct ccggcagttc ccctcgttcc acgtcggatc cgagcagatc gcagaggatg
      901 cggcggaagt actcgtgcct cggataggag aggaagctcc tcgaatcggt cagcataccg
      961 acgaagcggg agagcaatcc gacagaggaa agggcgcgaa gctgcctcgt catgccgtcc
     1021 ttcgtgtcgg cgaaccacca ggcggagccc atctgcatct tgccggggat ctttccgtcc
     1081 tgaaagctgc cgaggatagt cgggatcatg tcgttgtcct tggggttgag cgagtacacg
     1141 atggtcttcg tcagcttgcc cgtccgttcg aggcggtcga gaaggcgtac aagcgcgcgc
     1201 ccctgttcga agtcgcccat ggagtcgtac cccgtgttcg ggccgagacg gctgaacgcg
     1261 cgggaattgt tgttgcgcat ggctcccagg tggagctgct gcacccaccc ctggttcgcg
     1321 tccatcgtga gcatctcgaa gagaacggcc gaacggaagc gcgcaagcgc gtcccccgtg
     1381 acagttttcc cccttcgcag cgccgcaaag gagccctcta catcctcggt ggtccattcg
     1441 actgagtagg gagtctcgat tccgtagtcc gagagtcggc atcccttgcc gtgaaagtac
     1501 tggtgcctgc gccagatgca ctcgatgaaa cgtccgaagg agtccacttt tacgtcggtg
     1561 agttcggcga gcgagtcgag ccacggattg aactggtccg ggaggtgggc gtcgagggcg
     1621 cggtcggggc gaaaagcggg cgcgacgagc gtctcgaagc cgctcttttt gatggcctcg
     1681 tggtgttcaa gcgagtcggc ggggtcgtcg gtggtacaca gggcggcgac gttgaatttt
     1741 cggatcagcc cgcggaccga gaactccttc ttccggagca tcttcgaaca gtggtcgtga
     1801 atttcggcgg ccgtgtccgc gttcaacagc gtctcgacgc cgaagattcg ctgcagttcg
     1861 aggtgcgtcc agtggtagag cgggttgccg atggtctgag gaactacctt cgcccacgcg
     1921 gcaaacttct cctcggggga ggcgtcgccg gtgacgaagc gctcgtcgac gccgcacgcc
     1981 ctcatagcgc gccacttgta gtgatctcct cccagccacg cctcggcaag ggaggcgaac
     2041 tgtgtatcgc ccgctatttc ctccgcggga atatggcagt ggtagtcgaa tatcggcatc
     2101 ggggcggcgt attcgtggta gagcttttgc gccgccttgg aatgcagaag gaaatcctta
     2161 tccagaaact ttttcgccat agcgtctcct ccttgcaaat ggtttcatac tctgaaaatg
     2221 tattttactc tatgataaga atttttgcac ggggaaatcg taggaactat tcggcgggag
     2281 gctccttttc cggaaggtgt ttccaatatt ttttcggcat gaactgtctc tggagtctcg
     2341 ggttctccct cgaccggacg gcgatggagt ggaaatactc tttccacagg tcgcggaatg
     2401 cttcctcccg gcgggacggg gcgattcctt cggggaggct gaagtcggcg atgcgccaga
     2461 ggcgcggagc gtagatcgct gcttttcccc tcccgaggtc gtggatgatc cacttctcgt
     2521 ttcctaacct cgttgagaag tgcccggcga gcagctgaag gagatcggcc tcgggctcga
     2581 acggagcgta gagcacgccg ccgatctcgg tgaagcggag aagccccagg aaacggtggc
     2641 gctctctcag gacggcacgg ctcgcttcgt ggaccggcag gacgcgcgga tcggtcagca
     2701 gtctgtccac gctcttgccg agcgtccacg cgagattgta ataggtatag accgacattt
     2761 cgcgttcttt gagcgcgctg agaaaggcgt agtaccctcg ggtgaataca ggggccgaaa
     2821 gacgacggcg caggacgtcg ccggtctgct tcgcaagcag ctcatctgtc cgggcttctt
     2881 tgggcggcca gagaaacggc tgtccgctgc ccgcgtgctc gatcgcctcg ggcggttctc
     2941 ctgcgcgcgt actttcgaag agcagacaga ggaatccgtt gaagctgccg tcgtagaccc
     3001 aggtggccac tcgtcagaac tccagcgaga gctggcgggt cttccacgcg gggggcggag
     3061 gaggagtgag aagacggcgc agcgcccgcg catcaaggga ggggagggag aatcccttgc
     3121 aggcgaggaa gtagcgcgcc cgcttcatca ccactccgag cttcttcagg tcgtcctcgt
     3181 cgaggcgtcg tcccttccgt gccgtcagaa tgcgttttgc cgagaggacg ccgatgcccg
     3241 gggttcgcag cagctcctcg taggaggcgc ggtttatgtc cagcggaaag aggtcgagat
     3301 ggcggagcgc ccatgacgtc ttcgggtcga gcgactcgtc aagattcggc tgctccggcg
     3361 agaggacttc gtccggacgg aatccgtaga agcggagcag ccagtcggcc tggtagattc
     3421 tgtgttcgcg caggagcggc ggcgttgtgg tgatcgcggg gagaagcggg ttcgccgaga
     3481 cgggaacgta cgcggagtag tagacgcgct tgagaccgaa acggtgatag agtcctcccg
     3541 ccaggctgag gatggagagg tcggtctccg gagaggcgcc cacgatgagc tgcgtgctct
     3601 gtcctgcagg gacgaacggc gcgagcgaac gtgtggaaac gaggcgtttc tccttccgtt
     3661 cagcgatgga gtcgccgatg aggctcatcg gacgaaagat ggactccttt gtcttttggg
     3721 gcgcgagggc ccgcagactg acctccgaag ggagttcgat gttgacgctc atcctgtcgg
     3781 caaggaaacc gagcgcggtg accaacctgg gagacgcacc cggaatggcc ttcgcgtgga
     3841 tgtagcctcc gaaccggtac tcatcgcgga ggatcttcgc cgtgcggatg agctgaatca
     3901 tggtgtcgtc gggggaaccg acgaccgcag aactcaggaa gagcccctcg atgtagttcc
     3961 tgcggtagaa tccgatgacg aggtcggcga gctcacgcgg ggtgaatgat gcccgaggca
     4021 cgtcgttgct tcttcggttg acgcagtagg cgcagtcgaa ggcgcagtcg ttggagaaga
     4081 ggatcttgag cagcgagatg caccgtccgt catccgacca gctgtggcag atgccgccaa
     4141 gcgatgtgtt gccgaccgct cctcttcgtc ctccgtttcc gctggaggag caggaaacgt
     4201 cgtattttgc ggcttcggca agaattgaca gcttaccggc ggcatccatt cttttcgccc
     4261 ccttcctcaa ggttatgcta accaaatgtt atcattattt cgcggaaatg aaaagccctc
     4321 cggaaaactg gggaagtggg cagatcgttg aacagcattg cgtgtgtgcg tacgaaaaaa
     4381 tggaggatgt tttgtatcca tgcctcgtct gcgctgaaaa gtttgctata atggctaccg
     4441 gagtccgagc tgaagaagtg ccgcacgtat ctggttgctg ggaggatgaa cgctttgaag
     4501 gctttttcct cgttgaacgc cgattctccg aagcgtaccg gacgcatgga atctattcac
     4561 tggtacgtgt ttttcgtctt gctgcttgta ggggctgtat ttttggtcat ggagctgctc
     4621 catgcccgcg cagtccgcaa gcaggaacag tttttcagcg accgtcagta ccttcaaacc
     4681 accatgtcgc gtctcgcgtt agaggatcgg gtctcctccg ttctcatgca agaacggttt
     4741 cttttggagc accttcttct agagcctgca aacgggtcct tccctcctct atccgacatt
     4801 caacgggcgc ttcaaagtgc catttacaac accgccgata tcctgggctt ctatttcgcg
     4861 ccccacccgc tgtactccgc cgcgcagcgg ttcagttcgt tccgttcgac cccgaccgga
     4921 gacgcggcca gggagtttgc cggttctctg gaggattctt ttgatttttc cactcctcgt
     4981 cccgacgatc tctaccattt ttcaggaagc gtccgggcgt cctcgcgcgt caggatgatg
     5041 gcgctggttg aggcggctgt atcggaaaaa ggctttatgg ggtacttcat cacgatcatc
     5101 gatcttaccc ctttccttgt cagatatgtg ctccccatgg cccagggggc agggagcgcg
     5161 gtcttcctca tcgacaggga gggagtggtt gtcgaacatc gggattcttc tctggtgggg
     5221 ggcaacgcgg aaaagctctc gggaggagag ggggatgccc ttcgggtggc gtggtcgcgg
     5281 gtcagagagt ctttttccgg aatggaggct atcgagcttt ttgatgtctc ttccgggatg
     5341 ccgcagagga aacggctggc atggaattcc gccagggtag gggagaacag gttgtttgtc
     5401 ggcgtctacg ctcctgagtc cgatatcaac ccggtggttt ccgaactgcg ggtgcagcgc
     5461 ttcattctcg gaggggtact cgcgctcttg ctggtcatga ttgcggttgt ctttattcga
     5521 aaacgttccg acttcgaagt ccggcggagc gaatcgacat tcagggcaat tttcaacagc
     5581 gccgcgtccg ggatagcgat tctcgatgcg aacggggtta tcctttcctg taatacaacg
     5641 atggaaaaca tgacggggcg cccctccgcg cagttgagga tgcgcacgct cttcgacctc
     5701 gcggcgccgt cgttgcagga ggagaaggaa aaagtgcggg aggctttgcg atccggcgcg
     5761 ctctcggcga gggctgaact ccttttctca aggaagggag ccgaagctac aagtcccgag
     5821 aatggatcct tctggggaga tgtttcgctg aaccgcctgg aaagagactc gtcaaccgtc
     5881 caaatgctcg cggtggtgac tgatatttcg ggattgaaac gggcggagga cttgctccgg
     5941 caaaacgcgg aaacgctgga agcgcggaag aaggagcttg agcatgttgc ggccagtcaa
     6001 agcgccctgc tggagatgtt tacgttcttt tccgagtgcg tgacgataga agaacttttc
     6061 caggtgctct ccgaacatct tttcccggtg attccgttcc gctgccagac gctcttcatc
     6121 aagcgctcgc gtgaagccgc ttcgttcgaa gtgctcgact cgggaggaga tgtgctgcgg
     6181 tcgatgcaga cggatttcct cgaaaagaag aagggaattc tcggctacgt cgccaacacg
     6241 ggcaaagcct acctcatggg cgatgtgtcc atcgatccct atttcatacc ccattcgccc
     6301 gaggtgcggt cggccgtcgt cgcccctgtg atgtacaagg gattcctctg gggagctatt
     6361 gtgctggaca gcgacaggaa gtttgctttc ggcatacggg agagggatat gctgagcatc
     6421 gtgggttcct acgtcgcgct ccacctcgag gagttttttg ccagacagga actggataag
     6481 aaggcgacgc agctgcgctt tctccacagg gtgatccagc agatcgctgc agagcgaaac
     6541 aacgaggcgc tcgcacgcaa gattgtcgat gtgctcttcg acgagctcgg tttccccgga
     6601 gccggattct acactcctcc tccgaaggga gagaacgctc cggttctggt tgccggcaga
     6661 tcgagggcgg agcgagaaac ctgttccccg gagtgcgaac tcgaggcggg agctgctatg
     6721 agaagccgca cccccgtcga gcgggaagtg aacggcagag cgctcgttct ctccattccg
     6781 gtcacttttg aaggagaggt cttcggcgct ctcgccgccc ggcacgacgc cggtttttcc
     6841 gcagcggacc gggagctgct cgagatcacg gcagagcaca tgacgacctt ctgggcgctg
     6901 aacaatctgc ttgcgcaacg ccgccacgag tcgcttgtcg atcctttgac gcaggtatgg
     6961 aacaggcggt acatcatgca ccggcttgac gaagagagtt cccgcatcaa caggacgaac
     7021 ggcagaggct ccgtgattct cgtcgacctc ggcgacttca agcagatcaa cgaccgttac
     7081 gggcacgtta cgggggacga ggtccttcgt gtgacatccg ccacaatgag tgaaaacctc
     7141 aggaattacg atatgatagg gcgctacggc ggagatgagt tccttctcta cctgccggac
     7201 gtctcacgag aggaagccgg ggcgatcatg gtacggatgg agctgaaggt cgccgagcaa
     7261 aaaatcgaag gctttgaggg agcggtcatc cttgattacg gcatagccgg ctgccctgag
     7321 gatggaataa acctctcgga agtcatcagg attgcggatg aaaggatgta cagatacaag
     7381 gcggagagaa aggcggtgaa gagtgaatga tcgtatcgct gcatcatgcc agttttacgg
     7441 tggagaatct cgaaaggtcg gtcgcgtttt atcaagaaac gctggggctg aaactcgaag
     7501 gagtgtggga gcgggatcag gaatactccg agggcgtcac cggcatacgc ggggcatggc
     7561 tcaaagtggc ctatttttct cttccgaacg cttcttttga acttgtggag tacctggggg
     7621 cgaaggggaa gaagatcgac acgtcgacaa ataatgtcgg aagcgctcat gtgtgcctta
     7681 ttgttgcaaa tttcgacgcc ttcgttgagg cgcttgcgga acgcggcgtc gtttttgcag
     7741 ggaaaacctg cactatccct gccggttcga acaagggaaa gaaaatcgcc tatatcgagg
     7801 accccgacgg aaataccctt gagctggtgt catcgagagc tctttcgtaa cacgccggat
     7861 gcgaaaaccg gaaagaacca gcaaaggagg ataaaaacgt catgcttggt cagtattatt
     7921 tgattaccgg gataggtgct tttgctctga ttctcctcgc cgtgatcacc gggttgttcg
     7981 gcagaaacct caggaagatc gtccccgggc cccttgtgct gaaaatccac aagttctcgg
     8041 cccttacagg cgctctttgc gctcttctgc acgtgctcgg cgttcacggg tattagcagc
     8101 gtccgcctgc cggcttcaca atgcgtcttt attctccggc gtcacagttt accataaaga
     8161 aaggtgattt tcagtgcttc ctatgccaag tcagaagatc ctcgtcgtcg aggactcgct
     8221 cagtcagcga atgatgatct cccgccacct ccgtaactgg gagtacgagg tgctggaggc
     8281 cgccgacggc gtagaggcgt tcaccgcgtt tcggcagcag agccccgcga tcatcataac
     8341 ggacctcgat atgccggaaa tgaacggctt tcagcttatt gaggagattc gcaggaagga
     8401 gatagcccgt acgtacatca tcgtcctcag cgcctccgac gacaagtcga gcgtcgtttc
     8461 agccctggcg ctgggcgcgg acgactatct cgtgaagccg tatcaccccg atgaactgcg
     8521 cgtgcgcctg acaggagccg agcgcgtgct gcgcctccag aaccaggagc tgctcatctt
     8581 cgccatggca cagatggtgg acacccgaag tcccgagaca ggagcccacc tcgacagagt
     8641 gcagtatttc agcaggcgca tagcggaaga gcttgccgcg gaaggagatc cgcttgttac
     8701 ggcttcgtgg atagcgcagc ttttttccct ttctccgctt catgatatcg gcaagatcgg
     8761 catccccgac gccatcctga acaaaccggg cagactgact cccgaagagt ttgtcattat
     8821 gaaagagcat gttaccatcg gcgcctcgat cttctcgcgg atctacgaac gtactcacta
     8881 tgcgccgttc ggattcgcca tcgatattat ccgctgtcat cacgagaagt gggacggcag
     8941 cggatacccc gacgggctcg cgggagagag cattccgctc tgcggaagga ttgtcgctct
     9001 cgccgacgtg tacgacgcca tcagcagcaa gcgtgtctac aaaccatcct tcccgcggga
     9061 gcagtgctgg gatattatca acaggggcag gggaagccat ttcgatccgc ggcttgtcga
     9121 catattcaac cgcaacgagg attacttctg gaatatcgtc gaagagctac gggatatcga
     9181 cgattgagtt cgccgtaggt gacgggaggg ggatcttttt atgtacggac agttctcccg
     9241 ttttgtcggg cgtatttcgc tccggaggat cattcgcatc gcttttgtct ttcagatact
     9301 cattcctgtc tctttcttgg ggtggatgtt cttcagaacg tcccgggaat cggtcgaagc
     9361 gctctctatg cgcctgggga gcgaagtggc ggcgcggatc gcggagcatc tccagtcgta
     9421 tatggctgtc cctctcctcg tgaactctct caatgcggac gcgctggagt cggggagggt
     9481 cgacatcgcc tctccccgga tctggcagcc ctttttcacc gaacgcatca gggcctttcc
     9541 gtcaatagcg tataccttca tcggcacgcc ccgcggagag ttcttcgggg cgcgccgctt
     9601 caaggagaat gtcgaactct tcgtagcaag tcccgagaca acgggaggcg cttccgtcta
     9661 ttactccgtc gatccccggg ggatgacggg agacgaaatc atctcctacc ccggcttcga
     9721 cccccgcacc cgtccgtggt atacagcggg cgaacacgcc gacactcccg tctggagcga
     9781 aatctaccgc cattttgcac tcgatgacct ggcgattacc gccgtccagg ctcttcgcgg
     9841 ggaggacggc tctctggtcg gagtgctcgg cgtcgatctc cccctctccg ctatcaacag
     9901 ctttctcagg gagctgaagg tgggcgccga aggacagatt ttcatcctcg acaatgacgg
     9961 aaacattgtg gcggattcga ctgtcgataa gccgtttcac atccgcaacg gcgccttttc
    10021 ccggattgcg ggcgtggctt ctcccaatcc ggcaataagg gcggccgctg aaaggatggc
    10081 gcgcgaagag cagggagagc gcacaggccg aatgttctcc gtcgaaaact ccgcaggtat
    10141 ttccgagtac gtccggacac gcccgttccg atctctcggt ctcgactgga agatcgccgt
    10201 cgtcattccg gagaaggagt tcatagggtc tgtgcagcgt atggcccgct cggcgtttct
    10261 gatcaccctg gctgcgcttg gtctttcttt gctgacggcg ttctgggttt cccgctggat
    10321 atgccgtccc ctttcggatc ttgtggaatc ggcgaaggct atcacccgcg gcgagtggcg
    10381 cgctccgttc gtcctgaagc gggacgacga ggtgggagag ctctcccgct ccttcgccct
    10441 gatgtcgaaa cagctttcgg aggcgttctc cggccttgaa gagaaggtcc gcttacggac
    10501 ggtcgaactc gaacagaaaa accgggagct gaccgaggcc aaggcggttt cggagtctct
    10561 cgctgaggag gcgaaagcgg cgagccgcgc gaagagctcg tttctggcct ccatgagcca
    10621 tgaaatccgc acgccgatga acgcagtgct cgggttaagc gacctcgtgc tctcaacgcc
    10681 gctctcagag gagcagcgag aacatctttc tcttgtgcag acatctgccg agtcgctgct
    10741 tggtattctt gacgatattc tcgatcttgc gaagatagag gcgggaaaga tgtcgctgga
    10801 gaaccggccg ttccgtctgc gtcctctcgt ggagcaggtg ggagcgctca tgcgccctcg
    10861 cgcggagaaa aacggagtgg cgcttggagt gtcggtagcg ccggagctct ccgacgcctt
    10921 cgagggagat ccgctccgcg tgcggcaaat tctgatgaat cttgtcggca atgccgtaaa
    10981 attcacctcc gagggctctg tggagatcgt cgtgacgctc gcgggtgggg atgaggagca
    11041 ggcgcaggtt gcgtttgcgg tcagcgacac cggcccgggg attccgcaaa gcaaagtgca
    11101 tcttctgttt gacgatttta cccagttgga tggttcttcg acccgaaaac acggagggac
    11161 cgggctcggc cttgcgatat cgcgcagcct cgcccgcctg atgggcggcg atatcactgt
    11221 ggagagcgaa gtcggcaggg ggagcgtctt ccgcttttct cttccccttc ttcgctgctc
    11281 cggagagatt gttgaggaac atgagggtac ggaaacggat ttctcatcga tgaagggtaa
    11341 cgttcttctt gccgaggata acgccgtcaa tacgaagatg accgtcgcga tgctcaaaaa
    11401 aacaggactc acggtacggc acgctcaaaa cggccgggag gcggtcgaga tgtggcgcag
    11461 cgaaccgttc gatctgattc tcatggattg cgagatgccg gtgatggatg gattggaggc
    11521 gacgaggagg attcgctccg aagaacagga cggaaagagt attcctattg tagcgctcac
    11581 cgcgtatgcg atgaaagggg accgggagcg atgcctggag gcgggcatga cggagtatat
    11641 ctccaagccc attcggtccc gcaagctgta ctcgttgctc gccagatttc tcgggacacc
    11701 ctctccctct ctgcacaccg acagcgaaga aacgcccgcg cggttttctg ccgaaaatgg
    11761 agagcgggag cgctgggaga gcgggttcgc cgaactgctc ctctctctcg acggagaccg
    11821 ggaagcgttg gcggagatgg tggaggcctt cttttccgaa gtcccggagc tgcgcggctc
    11881 gcttcggagc tccctggaaa agggcgattc ctccgcagca gcgcgcgcgt tgcataaact
    11941 caagggagta cttggctatc tcatgggagg agaggacatc tcattattcc gcgttcttga
    12001 gattgatgcc aggacggagg gtttgaaaaa ggacgatccg cgccttcgtg aactgcacga
    12061 cctgctcgac cggtacgaac tctttcttcg tgaagcggct tcccgctgag catccgttgt
    12121 gcgcttattc cggggaattt tctcagcttc ctcactttcg attgaaaagg ccatcttttc
    12181 ggacgaaacg cttcggggta taatcctctc caatctttcg tccaggaggc attgctatga
    12241 atccacagtt tgcacgaaga atgacgcgct ttcaagagtc cgatctgacg cccatcctgc
    12301 gtcttgcagc aaatcctgaa gtgatctcgt tcgccggagg tcttccggcg ccggagctct
    12361 tcccggtcga ggatttgaag aaggtggccg tgaaggtgct cgacgccaac ggcagacggg
    12421 cgcttcagta ctccgccacg gaagggcatc ctcccctccg cgagaagatc gccgcgcgca
    12481 ccaacagaaa cctgaagacg tcgctgacgg ccgggaacgt cgtgatcaca accgggtccc
    12541 agcagtcgct ggatattctg gcgaagatgt acatcgacga gggggactgc attctgtgcg
    12601 agagtcccac ctacctcggc gctctttcgg ccttcaactc gtgccttccg gcgtaccggg
    12661 aagtggcgac ggacggcgag ggaatgatca tcgccgatct tgagaaggcg ctcgcctcgg
    12721 agaagcgcgc gaagatgatc tacgtcattc cggacttcca gaacccctcc ggcaagacgt
    12781 ggtcgcttga acggcgcaag gcgtttctgg ataccgtctc gaagtacgat atcctggtgg
    12841 tggaggacaa cccgtacggc gagctccgct tcgaagggac gacgcccccc tctttgaagt
    12901 cgatggatac ccgcggccag gtcgtgatgc tgggcacctt ctcgaagatc ttctgccccg
    12961 gcgtccgcgt ggcatgggtc tgcggggagc ccgcgttcct tgagagcttc tgcaatctga
    13021 aacaggccgc cgacctccac acgtcgacga tca
//
LOCUS       DAOPKU010000013        12907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_5102_length_12907_cov_5.348739, whole genome shotgun sequence.
ACCESSION   DAOPKU010000013 DAOPKU010000000
VERSION     DAOPKU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5102_length_12907_cov_5.348739"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PK849_00855"
     CDS             <1..628
                     /locus_tag="PK849_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE64699.1"
                     /translation="TYMPQSEETGYLSGLIAGMTTKKNIVGLVGPVDGGDAARYNRGF
                     VLGVHAVNPEAKIMVAHTGSFADFVKAGEVAETQIKAGADVLTGSSQQALGALRAVAD
                     HKDAPIWWVGQDIAQIHITEGYKCIAASSYNYAAVIVGLVAKLDAGVRGGECIPMNFS
                     NNGFVFAFNETLKDMYTGAIEQKVNEALEKFRATPETLNWSAVDYSKL"
     gene            696..2234
                     /locus_tag="PK849_00860"
     CDS             696..2234
                     /locus_tag="PK849_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64700.1"
                     /translation="MEPNTRKITSLRVEHITKRFPGVLACNDISLSVAEGEVLALVGE
                     NGAGKTTLMNILMGLYRPDEGRILINGEEVVFRSPNDAFAAGLGMVHQQYMLVANMTV
                     LENIALGFTQAWSPLKLNLDMVRARIDEVAAKYGLAVDPDAFVWQLSVGEQQRVELVK
                     TLCLGARFLILDEPTSALTPQETDELILLLKRMTSELSIVFISHKLHEVKNLSDKVTI
                     LRHGSVVFEGSTADHSSGDIAALMTGREIHLPRNEAPTCEGNPALEIRGLKVRSDRGF
                     LAVDGLDLSVCAGEIVGLAGVSGNGQRELAEAINGLRKVEGGEILLFGQELANRSPRA
                     VIDAGMGYIPEERNTEGIVPSFSMKENFILKDADEAQFSARSFLRLKEIDRNAEALRT
                     EFDIRSPDTKVAAGSLSGGNIQKVILAREISRKPRFLIAVYPTRGLDMGAAEFIHKRL
                     LEKRLEGIGILLISEELEEILDLSDRVAVIFKGRIIDTLDRRSADSRKIGILMAGVHN
                     DEAV"
     gene            2218..3315
                     /locus_tag="PK849_00865"
     CDS             2218..3315
                     /locus_tag="PK849_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64701.1"
                     /translation="MTKQFKALYKTVVILLAAAAAMLLGALILWTIGADVTKTYSVII
                     LEPLKNKIQIGEVLIRAIPLTIIALGISVAYRSGIINIGAEGQMAMGILAATAVALAL
                     PGLPKYALLPLAMIAGTLGGAAWGFIPGILKAKLHVSELLSTVMLNYIAAQFYTFCLR
                     GPMLDPAEITMGSGTPQSMRLTKAIWLDRLVPGTRIHTGVYIALAMAFIIWLLLWRTS
                     WGYKMRAAGASERAARYGGIGVAGYLVVAMMISGAFAGLAGAVEIAGVHRRAIEGITG
                     GYGFSGIVVALFGGLHPAGIIPASFFFGLLIVGADMTQRMVGVPANMVLVLQGIIILV
                     IVATRMVLANPYLMERVWRRYQKLTRRDKEA"
     gene            3320..>3642
                     /locus_tag="PK849_00870"
     CDS             3320..>3642
                     /locus_tag="PK849_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64702.1"
                     /translation="MDFVVNIFALGIPFSVALLLASLGEMFNQRAGVFNLGCEGIMAM
                     GAFLGMLVPYSVGQGGPVSGAYNILGLLLAMGIGALLGLFFGLVVVTFRAPQGIAGIG
                     LQMFGV"
     assembly_gap    3643..3742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3743..4419
                     /locus_tag="PK849_00875"
     CDS             <3743..4419
                     /locus_tag="PK849_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64703.1"
                     /translation="TFRAPQGIAGIGLQMFGVGAAGTLFRHFVGGTQSIPGIDRLPIP
                     VLSEIPVLGPVLFSHNPLVYMTFLMVPAAWYVLFKTPWGLRVRASGTNPRAADSIGIK
                     VNLVRFQALAVGGALAGLAGAYLSLCQVKMFSDEIIAGRGFIAVALVYFGHWRPLKIM
                     GGALLFSLAQALQLAIQGQGIDFPYEFAVMLPYAMVIVVLAFSRESLLLGPTALGKPF
                     NREMRT"
     gene            4497..4982
                     /locus_tag="PK849_00880"
     CDS             4497..4982
                     /locus_tag="PK849_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPE64704.1"
                     /translation="MNTSHETLLRRCVEISKEAVAAGNHPFGALLADKDGNILVESGN
                     IEVTERDCTGHAETTVMRLAGKKYSKEFLWECTLYTTAEPCCMCVGAIYWGNVGHVVF
                     GITEKQLLELTGSHEDNPTFDLPSREIVARGQKKIEIIGPVADEALQEEIIAVHRGYW
                     K"
     gene            5081..5524
                     /locus_tag="PK849_00885"
     CDS             5081..5524
                     /locus_tag="PK849_00885"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPE64705.1"
                     /translation="MPLMTNSSPVCSSAAMDSSWTKRRNNAIRRRCEQFACSLETLFG
                     DDLLAVWLYGSCATKTNHAESDVDVAIILTEAGKKRYEQNTDGIASDCDDLEFSMARE
                     LAMPVSLLIWSEERLSREIAFCTGFASRLLEKNVVLFQKRGWMPK"
     gene            5521..6051
                     /locus_tag="PK849_00890"
     CDS             5521..6051
                     /locus_tag="PK849_00890"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPE64706.1"
                     /translation="MMVKTLTGANDSPEIRERLVLKALRVSLGHLNGCVLMIELNEAV
                     MANDHLCYALFHAAKAALFARGKDPSTHQGVKMELSGVASENGLDREFIRFFGNRERL
                     RSEMSYSYLGEVETFSIQEVTTALQASHRWMKTLIDELVRPLIPSVPQFDELVRSSET
                     GNSLLGDKGLWSVLHE"
     gene            complement(6249..7451)
                     /locus_tag="PK849_00895"
     CDS             complement(6249..7451)
                     /locus_tag="PK849_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE64707.1"
                     /translation="MLEKIKKLATAYGDEVVAFRREFHMHPERSWEEVETSKKVVEAL
                     KGLGCTIVKTGFGGTESGVVAEMKGGKPGKCVALRADIDALPLQEENKVPYASQTPCV
                     MHACGHDAHTAILLGAAKILAEIRDELPGTVRFIFQPAEECGLRSGAKVMLDEGALDG
                     VDSIGGLHVWSLAPTGTALYRSGPVMAAADAWYLTIKGKGGHGSAPENSIDPTITAAN
                     LVLNLQAIVGREVSARETAVVSTGVVKAGETVFNIIPDSVTMNGTVRTFNKEVQDTIE
                     AAIRRVIAGLCAAGRCEFELDYRRNLPATVNDHEVTMTAKAVCEYVLGADKVKETELI
                     MGSEDFSYFEQKVPGTFFFLGTGNPEKETNNPHHHPRFDLDEDALPAGSAMLAGFAWA
                     QLTGEVRG"
     gene            7829..8224
                     /gene="rbsD"
                     /locus_tag="PK849_00900"
     CDS             7829..8224
                     /gene="rbsD"
                     /locus_tag="PK849_00900"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HPE64708.1"
                     /translation="MKRSGILHPELAFQIASCGHGDLVCIADAGLPIPPETPRIDLAY
                     ALGQPGFFNVLEAILPELAIEGAVWAEEALAKSPMLAGRLETLFQQTPHERIPHEEFK
                     KLLPDVRFVVRTGECTPYANVLLRCGVPF"
     gene            8240..9187
                     /locus_tag="PK849_00905"
     CDS             8240..9187
                     /locus_tag="PK849_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64709.1"
                     /translation="MPIFRRFDSRTLLLLLLGGLMLVAGSLEPRFFTGANLLNVLRQS
                     SIIGVISLGMTVVILTGGIDLSVGSVLSLSVLFAASASKTGLPAPVAWACALLAGAGL
                     GWINGFGVSRLRLPPFIVTLGMMGFARGLSFIYTGGAPITGFDALFRLPGTGVFAGIP
                     APALLFGAAFIIVFVLLERCPWGEHIRNTGSNPVAAWGSGIDVPGTISKAYVLCGLLS
                     ALAGLMLVGRLDSAQPGAGVGYEFGAIAAVVLGGTSFSGGKGTLAGTILGVLIMGVLE
                     NGMNLLNVNPFSEQVVKGVVIAAALVAYGSLGRKSRTAG"
     gene            9355..10233
                     /gene="rbsB"
                     /locus_tag="PK849_00910"
     CDS             9355..10233
                     /gene="rbsB"
                     /locus_tag="PK849_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019259653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HPE64710.1"
                     /translation="MKKLFAVLLVVLLVAAPAAAAEKTLGLVISTLNNPFFVTLKQGA
                     EEAATKAGVKLVVLDAQDDSAKMTAAMEDLIQQKVDAILVNPTDSDAVTPSIMKANAA
                     GIPVLTIDRGASGGQVLCHVASDNVAGGVMAGEFLAKKLEGKGNVVELVGIPGTSAAR
                     DRGKGFNDAIATFKDIKVVASQAADFSRDKGLKVFENILQAQPEIAGVFAHNDEMILG
                     AVAAAEAAGRTGIVFVGFDAVDDAVAAVKAGKLAATVAQQPAVIGAMGVEKAVAHLGG
                     EKLPEFIPVPLQLVVE"
     gene            10305..11813
                     /locus_tag="PK849_00915"
     CDS             10305..11813
                     /locus_tag="PK849_00915"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPE64711.1"
                     /translation="MTLLLEMKNVHKRYPGVHALKGISYSLRAGELHALVGENGAGKS
                     TLVKVLGGVVAPDEGKILIRGESARWSGPGDAIRAGVGIIHQELSLASHLTVAENIYL
                     GREPKRGPFIDRRKLADDAARLVEGFGVGIDVKRRAGTLSPGEQQLVEIAKAISQNVS
                     ILAMDEPTSSLSARETEQLFRVIAGLRASGTGIIYISHHLEEVFLLADRISVLRDGEM
                     AGSADAKELTPERVVSLMVGRQIEALYPRTFRDPGAVLLDVRGLSRRGVLEGVSFTLR
                     SDEILGVAGLVGSGRTELARCLVGLDPFDAGEIAVEGTPFTPRSPGDALDRGIVLVPE
                     DRKRQGLFLNRTIRDNTISMRLLQGLRKGPFVDRPASESLAGSFVQRLGVRCRSVFDS
                     VFSLSGGNQQKVAIAKGLAVSPKILILDEPTRGVDVGAKAEIHQIMNDLAAQGMGILM
                     ISSDLPEVLGMSDAVLVFRDGRCVARFPRGEGAQGTLGERVMTAATGGIANA"
     gene            11806..12786
                     /locus_tag="PK849_00920"
     CDS             11806..12786
                     /locus_tag="PK849_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64712.1"
                     /translation="MPEKTQSFLATLRDKGWGSVLLLFAVVGLLAAFHPGVLDRQNMA
                     NVLAQSAIVGVCAVGMTFVLLSGGIDLSVGSVLALAGAVGAWTCKELGLPVPLAWAVA
                     VLTGTLCGLASGSLVAFGRVPPFMATLAMLAAARGGTLLLTQGYPVSGLPPAFYTPGW
                     ASVGPFPVSGLVFFGLALAAWVLLQRTPFGTHLRAFGDNPETARLAGLSAPRLTLSVY
                     AISGSLAGLAGVLMVGRLWSAQPNVGMGLELDVIAAVVLGGASLFGGVGGVGGTVAGV
                     LIMGFLDNGLRLLAISSYVQQSVKGLVFVGAVLLDLAFKGYYRKGRGRDA"
BASE COUNT         2270 a         4292 c         3963 g         2282 t
ORIGIN      
        1 cacctacatg cctcagagcg aggagaccgg ctatctctcc ggcctgatcg cgggcatgac
       61 gacgaaaaag aatatcgtcg ggctcgtcgg cccggtcgac ggcggcgacg cggcgcgcta
      121 caaccgcggc ttcgtcctcg gcgtgcatgc ggtcaacccc gaggcgaaga tcatggtcgc
      181 ccacaccggc tcgttcgcgg acttcgtgaa ggccggagag gttgcggaaa cccagatcaa
      241 ggcgggcgcg gacgtgctca ccggctcttc gcagcaggcc ctcggcgctc tgcgcgccgt
      301 cgcggaccac aaggacgccc ccatctggtg ggtcggccag gacatcgccc agatccacat
      361 caccgagggg tacaagtgca tcgcggcgtc ctcctacaac tacgcggcgg tcatcgtcgg
      421 ccttgtcgcc aagcttgacg ccggagtacg gggcggcgag tgcatcccga tgaacttctc
      481 caacaacggg ttcgtcttcg ccttcaacga aacgctgaag gacatgtaca cgggcgcgat
      541 cgagcagaag gtcaacgagg ccctcgaaaa gttccgggca accccggaga cgctcaactg
      601 gagcgccgtc gactactcca agctgtaatc cgcatgtatg tcctgcgggg gcgccccctc
      661 cgcaggacat ttttttctct cgggaggtct cccgcatgga accgaatacc cgcaaaataa
      721 ccagtcttcg agtggaacac atcaccaagc gcttccccgg agtgctcgcc tgcaacgaca
      781 tctcgctctc cgtggccgag ggcgaagtgc ttgccctcgt cggagagaac ggcgccggaa
      841 agacgacgct gatgaacatc ctgatggggc tctaccgccc cgacgaggga cgcatcctga
      901 tcaacgggga ggaagtcgtc ttccgctccc ccaacgacgc attcgccgcc ggacttggga
      961 tggtgcacca gcagtacatg ctggtggcga acatgacggt gctggagaac atcgccctgg
     1021 gcttcacgca ggcgtggtct cccctgaagc tgaacctcga catggtgcgc gcccgcatcg
     1081 acgaggtggc ggcgaagtac gggctcgccg tcgacccgga cgccttcgtc tggcagctct
     1141 ccgtcggcga acagcagcgc gtggagctgg tgaagacgct ctgcctcggc gcgcgcttcc
     1201 tcatcctcga cgaaccgacc agcgccctca cgccgcagga gacggacgag ctgatcctcc
     1261 tgctcaaacg gatgacgagc gaactctcca tcgtcttcat cagccacaag ctccacgagg
     1321 tcaagaacct ctcggacaag gtcaccatcc tccggcacgg ctcggtcgtc ttcgagggca
     1381 gcaccgccga ccactcctcg ggcgacatcg cggcgcttat gaccggacgc gagattcacc
     1441 tccccaggaa cgaggcgccg acgtgcgagg gcaatccggc tctcgaaatc cgcgggctca
     1501 aggtcaggag cgaccgcggc ttcctcgccg tcgacgggct tgacctctcg gtctgcgcgg
     1561 gcgagatcgt cggactcgcc ggggtctccg gcaacgggca gcgcgagctg gccgaggcga
     1621 tcaacggcct gcgaaaagtc gaggggggcg aaatcctcct cttcgggcag gagctggcga
     1681 accgctctcc gcgcgccgtg atcgacgcgg ggatggggta cattcccgag gagcgcaaca
     1741 ccgaggggat cgtcccctcc ttctccatga aggagaactt catcctgaag gacgccgacg
     1801 aagcgcagtt ttcggcgcgc tcgttcctca ggctcaagga gatcgaccgg aacgccgagg
     1861 ccctgcggac ggagttcgac atccgcagcc ccgacacgaa ggtcgcggcg ggctcgctct
     1921 cgggagggaa catccagaag gtcatcctcg cgcgggagat ctcccgcaaa ccccgcttcc
     1981 tcatcgcggt ctaccccacg cgcggcctcg acatgggggc tgcggagttc atccacaagc
     2041 gcctcctgga gaagcgcctg gaggggatcg gcatcctgct catcagcgag gagctggagg
     2101 agatcctcga cctctccgac cgcgtcgccg tcatcttcaa ggggcgcatc atcgacacgc
     2161 tcgacagacg gtcggcggac agcagaaaaa tcggcatcct gatggcaggt gtgcacaatg
     2221 acgaagcagt ttaaggcgct ctacaagacc gtcgtcatcc tcctcgcggc ggccgcggcg
     2281 atgcttttgg gcgcgctcat cctctggacc atcggcgcgg acgtgacgaa gacctactcg
     2341 gtcatcatcc tcgaaccgct caagaacaaa attcagatcg gcgaagtgct catccgggcc
     2401 atcccgctga cgatcatcgc gctgggaatc tcggtcgcct accggagcgg catcatcaac
     2461 atcggcgccg aggggcagat ggcgatgggc atcctcgccg cgacggccgt ggcgctcgcc
     2521 ctccccggcc tgccgaagta cgcgctgctc ccgctggcaa tgatcgcggg aacgctcggc
     2581 ggggcggcct ggggcttcat tccgggcatc ctcaaggcga agctccacgt cagcgaactt
     2641 ttgtcgacgg tcatgctgaa ctacatcgcc gcgcagttct acaccttctg cctgcgcggg
     2701 cctatgctcg accccgccga gatcacgatg gggagcggca cgccgcagtc gatgcgcctg
     2761 acgaaagcga tctggctcga caggctggtc cccggcacgc gcatccacac cggggtctac
     2821 atcgcgctgg cgatggcctt catcatctgg ctgctgctct ggaggacgtc gtgggggtac
     2881 aagatgcgcg ccgccggagc gagcgaacgg gcggccaggt acggcggcat cggcgtggcg
     2941 gggtatctcg tcgtcgccat gatgatcagc ggcgcgttcg ccggactcgc aggggccgtc
     3001 gagatagccg gcgtgcaccg ccgcgccatc gagggaatca ccggcggcta cggcttcagc
     3061 ggcatcgtcg tcgccctctt cggagggctg cacccggcgg gcatcatccc ggcctccttc
     3121 ttcttcgggc tgctgatcgt gggagccgac atgacgcagc gcatggtggg cgtcccggcg
     3181 aatatggtgc tcgtgctgca aggcatcatc attctcgtca tcgtggcgac gcgaatggtg
     3241 ttggcgaacc cctacctgat ggaacgggtc tggcggcgct accagaagct gacccgccgc
     3301 gacaaggagg cgtaatccga tggacttcgt cgtcaacatt ttcgcgctcg gcattccgtt
     3361 cagcgtggcg ttgctgctcg catccctcgg ggagatgttc aaccagcggg cgggcgtctt
     3421 caatctcggc tgcgaaggca tcatggcgat gggcgccttc ctcgggatgc tcgtcccgta
     3481 cagcgtcggt cagggaggcc ccgtctccgg agcctacaac atactcggcc tcctgctcgc
     3541 catgggaatc ggcgcgctgc tcggcctctt cttcggcctg gtcgtggtca ccttccgcgc
     3601 cccgcagggc atcgcgggca tcggacttca aatgttcggc gtnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nntcaccttc cgcgccccgc agggcatcgc gggcatcgga
     3781 cttcaaatgt tcggcgtggg agccgcagga acccttttcc gtcacttcgt gggcggcacg
     3841 cagagcatcc ccggcatcga ccggctgccg atccccgtcc tttcggagat tccggtactc
     3901 ggaccggtcc tcttctcgca caatccgctg gtctacatga cgtttctcat ggtccccgcc
     3961 gcgtggtacg tcctcttcaa aacaccgtgg gggctgcgcg tccgcgcttc ggggaccaac
     4021 ccccgggcgg ccgactccat cggcatcaag gtcaacctcg tccgcttcca ggcgctcgcc
     4081 gtcggaggcg cgctcgcagg gctcgccggg gcgtatctca gcctctgcca ggtgaagatg
     4141 ttcagcgacg agatcatcgc cggacgcggc ttcatcgccg tggcgctcgt ctacttcggc
     4201 cactggcgtc cgctgaagat catggggggc gcgctcctct tcagtctcgc gcaggcgctc
     4261 cagctcgcca tccaagggca gggaatcgac ttcccgtacg agttcgccgt catgctcccc
     4321 tacgcgatgg tcatcgtcgt cctcgccttc agccgcgaga gcctgctgct gggccccacc
     4381 gcgctcggca agccgttcaa ccgcgagatg cgcacctagg aagagagacg gtcattgcag
     4441 cacatccgga ggggagccgc gcgctcccct cgtttttact tgaggaggag aagacaatga
     4501 acacatcgca cgaaacgctt ctgagacggt gcgttgaaat atcgaaggag gctgtcgccg
     4561 cgggaaacca tcccttcggc gcgctgcttg ccgacaaaga cgggaacatc ctcgtcgaat
     4621 cgggaaacat cgaggtcact gagcgggact gcacggggca cgcggagacc actgtcatgc
     4681 ggctcgccgg gaaaaagtac tccaaggaat tcctgtggga gtgcaccctc tacaccaccg
     4741 cagagccgtg ctgcatgtgc gtcggcgcga tctactgggg aaacgtgggg catgtggtct
     4801 tcggcatcac cgagaagcag ctcctcgaac tcaccggctc gcacgaggac aaccccacct
     4861 tcgacctccc ctcccgcgag atcgtcgcca ggggacagaa gaaaatagag atcatcggcc
     4921 ccgtcgccga cgaagcgctc caagaggaga tcatcgccgt ccacaggggg tattggaagt
     4981 agaggcgcgc cttcttggcg cctttcgatg cacgaagagc gcgcagtgta taataaactt
     5041 gatcaagggc tgcggagaac tcttggaagg gggcggagca atgccgctca tgacaaacag
     5101 ctcgcctgtg tgttcatccg ccgcgatgga ctcgtcgtgg acgaaaagaa gaaacaacgc
     5161 aatccgtcgt cggtgcgaac aattcgcctg ctcacttgaa acccttttcg gggacgatct
     5221 gctcgccgtc tggttgtacg gctcctgtgc gacgaagacg aatcatgcgg aatccgacgt
     5281 ggacgttgca attatcctga ccgaggcggg aaagaaacgc tacgagcaga ataccgatgg
     5341 tatcgcctcc gactgcgatg atctcgaatt ttcaatggcg agagaactcg cgatgcccgt
     5401 ctcgcttctg atatggagcg aagagaggct ttcgcgcgag atcgcttttt gcacgggctt
     5461 cgcctctcgt ttgctggaaa aaaacgttgt gctgttccaa aaacgggggt ggatgccgaa
     5521 atgatggtga aaacgctcac gggagcgaac gattctcctg aaatcaggga gcggctggtt
     5581 ctcaaggccc tccgcgtctc tctcggccat ttgaacgggt gcgtgctcat gatcgaactg
     5641 aacgaggctg tgatggcgaa cgatcacctc tgctacgcgc tgtttcatgc ggcgaaagca
     5701 gcgctttttg caagaggcaa ggatccttcc acgcatcaag gggtgaagat ggaactctcc
     5761 ggggtagcgt ccgaaaacgg attggaccgt gagttcatcc gttttttcgg aaatcgtgaa
     5821 cgattgcgct cggagatgag ctactcttat ctaggcgaag ttgagacctt ttccatccag
     5881 gaggtaacca ctgccctgca agcctcccac cgctggatga agacgctgat cgatgaactt
     5941 gttcgcccgc tgattccttc cgttccgcaa ttcgacgaac ttgtgcgatc ttcggaaaca
     6001 gggaactccc ttctcggcga caaaggactc tggtcggttc ttcacgagtg acggcgggtt
     6061 gccctggagg aggcaacagc gacagacttc aaaaagagcg tccggacgga gaaaaacagc
     6121 gtatgaagag tctttgcggc tcgctttccg cgtcgggatc aacctctatc aaaatatgcc
     6181 gtaggcgctt cgtcctcggg agttgtccgc cctcgggcga aacgccgtca aatttaccgg
     6241 aagccgcttc agccccgcac ttcccccgtc agctgcgccc acgcgaaccc cgcgagcatg
     6301 gcggaaccgg ctgggagcgc gtcttcgtcc aggtcgaatc tcgggtgatg gtgagggttg
     6361 ttcgtctcct tctccggatt gcccgttcca aggaagaaga aggttccggg gactttctgc
     6421 tcgaagtagc tgaagtcctc cgaacccatg atcagttccg tctccttcac cttgtccgca
     6481 cccagaacgt actcgcagac cgccttcgcc gtcatcgtga cctcgtgatc gttgactgtt
     6541 gcgggaaggt tgcgcctgta gtccagttcg aactcgcagc gtccggcggc gcacagcccc
     6601 gcgatcaccc tccggatagc cgcttctatc gtgtcctgca cctctttgtt gaaagtgcgc
     6661 acggtgccgt tcatcgtcac cgaatcgggg atgatgttga agacggtctc ccccgccttg
     6721 acgacgccgg tgctcaccac ggcggtctcg cgcgcgctca cctcgcggcc gacgatggcc
     6781 tggaggttga gcacgaggtt cgccgccgtg atcgtcgggt cgatggagtt ctccggagcg
     6841 gaaccgtgcc cgcccttccc cttgatcgtc aggtaccacg cgtccgcagc cgccatcaca
     6901 gggccggagc ggtagagcgc cgttcccgtg ggggccagcg accagacatg aagaccgcct
     6961 atggagtcga cgccgtcgag cgcgccctcg tcgagcatga ctttcgcgcc cgaccgcagg
     7021 ccgcactcct ccgcgggctg gaagatgaaa cggacggttc cgggaagctc gtcacggatc
     7081 tcggcgagga tcttcgccgc acccagcagg atggcggtgt gcgcgtcgtg gccgcaggcg
     7141 tgcatcacgc acggagtctg cgacgcatac gggaccttgt tctcctcctg aagcgggagc
     7201 gcgtcgatgt cggctcgcag cgccacgcac ttgcccggct tgccgccctt catctcggcg
     7261 acgacccccg actccgtgcc gccgaacccc gtcttcacga tggtgcagcc aagtcccttc
     7321 agcgcctcga cgaccttttt gctggtctcg acctcttccc aggagcgctc cggatgcatg
     7381 tggaactcgc ggcggaatgc gacgacctcg tcgccgtacg ccgttgccag tttcttgatt
     7441 ttttcaagca aaagaaatca cctcgttcaa tcgaatttta gaatacgaaa cagatatgca
     7501 aaaacactat cattcttttc cgcaccgcac aagcccccct cgaaacagat agctgaaaaa
     7561 catccggggt gtcgcgcagc ggcatctttc cgcacgcccg caccttccgc acatcacagt
     7621 ctgaagtagg tacaatgcac gaatttctct ggtcgaagac gcgtgattca cgttgtaaaa
     7681 accatgcctc cgtgctacga tacatgacaa tagcatgata aattacagcg cttctcttga
     7741 gtgaacgacg ggcgggcgca aggccacgga agtatgcgat agacggcccg cacgcttcac
     7801 catgagcgaa cgcaaggggg gacgcactat gaaacgcagc ggcattcttc acccggaact
     7861 ggctttccag atcgcttcct gcggccacgg cgaccttgtg tgcatcgccg acgcggggct
     7921 tccaataccc ccggagacgc cacggatcga cctggcctac gcgctcgggc aacccggctt
     7981 cttcaacgtg ctggaagcca tccttcccga actcgcaatc gaaggcgccg tctgggccga
     8041 agaggcgctc gccaaaagtc cgatgctcgc cggacggctg gaaacgctct tccagcagac
     8101 cccgcacgaa cgcattccgc acgaagagtt caagaagctc ctgcccgacg tccgcttcgt
     8161 cgtccgcacc ggtgagtgca ccccgtacgc caacgtgctg ctccgctgcg gcgttccgtt
     8221 ctaaagaagg cggaaggccg tgccgatctt tcggcgtttc gactcgcgaa cgctgctcct
     8281 gctgcttctg ggggggctca tgcttgttgc gggctcgctg gagccgcgct ttttcaccgg
     8341 cgccaacctg ctgaacgtgc tgcgccagtc ctccatcatc ggcgtcatct cgctgggcat
     8401 gaccgtggtc atcctcaccg gaggcatcga cctctccgtc ggcagcgtcc tctcgctctc
     8461 cgtcctcttc gccgcctccg cgtcgaaaac cggcctcccc gcccccgtcg cgtgggcgtg
     8521 cgccctcctc gcgggagccg ggctggggtg gatcaacggg ttcggcgtct ctcggctgcg
     8581 cctccccccc ttcatcgtca cgctgggaat gatggggttc gcgcgcgggc tctccttcat
     8641 ctacacgggc ggcgcgccca tcaccgggtt cgacgctctc ttcaggctgc cgggaacggg
     8701 cgtcttcgct ggaattcccg cgcccgcgct gctcttcgga gcggccttca tcatcgtctt
     8761 cgtcctcctc gaacgctgcc cgtgggggga gcatatccgc aacacgggct cgaaccccgt
     8821 cgccgcatgg ggatcgggga tcgacgtccc cggaacgatc tcgaaggcgt acgtcctctg
     8881 cggccttctc tccgcgctcg caggactgat gctcgtcgga aggctcgact ccgcgcagcc
     8941 cggcgcgggc gtcggctacg aattcggcgc catcgccgcc gtcgtgctcg gcggaaccag
     9001 cttctcggga ggcaagggaa cgctcgccgg aaccatcctc ggcgtgctga tcatgggcgt
     9061 gctggagaac gggatgaacc tgctcaacgt caatcccttc tccgaacagg tggtgaaggg
     9121 ggtggtcatc gcggcggccc tcgtggccta cggaagcctg gggcggaaga gccggacggc
     9181 ggggtagcgc ctgcttgccg tattccggac agccgcacgg ctcaaggaag tccgcgaaga
     9241 gcgcatgcac atcttgaacg attcatacgc gcttcccggc gtaccaaacc tgtttccaca
     9301 acgacgcgga ggcgtgaaac gccccgcgcg aaaattcaag gaggtgtccg tttcatgaaa
     9361 aagttgtttg ccgtactgct ggtggttctg ctcgtcgccg ctcccgcggc ggcggcggag
     9421 aagacgctcg gcctggtgat ctccacgctg aacaacccct tcttcgtcac gctgaagcag
     9481 ggggccgaag aggccgcaac aaaggcgggc gtgaagctcg tggtactgga cgctcaggac
     9541 gactcggcga agatgaccgc cgcgatggaa gacctgattc agcagaaggt cgacgccatt
     9601 ctggtgaacc cgaccgactc cgacgccgtc acgccctcca tcatgaaggc caacgccgcg
     9661 ggcatccccg tgctcaccat cgacaggggc gcgtccggcg gacaggttct ctgccacgtg
     9721 gccagcgaca acgtcgcggg cggcgtcatg gccggagagt tcctcgccaa gaagcttgaa
     9781 ggcaagggga acgtcgtcga gctggtcggc atccccggaa cctccgccgc cagagaccgc
     9841 ggcaagggat tcaacgacgc tattgccaca ttcaaggaca tcaaggtcgt cgcctcgcag
     9901 gccgccgact tcagccgcga caaggggctc aaggtcttcg agaacatcct ccaggcgcag
     9961 ccggagatcg cgggcgtctt cgcccacaac gacgagatga tcctcggcgc ggtcgccgcg
    10021 gccgaggccg ccggacgcac cggcatcgtc ttcgtcggtt tcgacgcggt ggacgacgcc
    10081 gtggccgccg tcaaggcggg caaactcgca gcgacggtcg cccagcagcc cgcggtcatc
    10141 ggcgcgatgg gcgtcgagaa ggcggtcgcg cacctcggcg gcgagaagct gcccgagttc
    10201 atccccgtgc cgctgcagct cgtcgtcgaa taggccgtac accagatgaa gtaccgggac
    10261 gccgtcgttc aagggcggcg tccctcccac cggaggttga tggcgtgaca ctcttgctgg
    10321 agatgaagaa cgtacacaag cgctaccccg gagtccacgc gctgaagggg atctcatact
    10381 ccctgcgcgc gggggaactc catgcgctcg tcggcgaaaa cggcgcgggg aagtccaccc
    10441 tcgtcaaggt actcggcggc gtggtcgctc ccgacgaagg gaagatcctg atacggggag
    10501 agtccgcccg atggagcggc cccggcgacg ccatccgcgc cggggtgggc atcatccacc
    10561 aggagctgag cctcgcgtcg cacctcaccg tggcggagaa catctacctc gggcgcgagc
    10621 cgaagcgcgg ccccttcatc gacaggcgga agctcgcgga cgacgcggcc cggcttgtcg
    10681 aggggttcgg cgtcggcatc gacgtgaagc ggcgcgccgg aacgctctct ccgggggagc
    10741 agcagcttgt cgaaatcgcc aaggcgatct cgcagaacgt ctccatcctc gccatggacg
    10801 agcccacctc cagtctctcc gcgcgcgaaa cggagcagct cttccgcgtc atcgccgggc
    10861 tacgcgcgtc cggaacgggc atcatctaca tctcgcacca cctcgaagag gtcttcctcc
    10921 tcgccgaccg catctccgtc ctccgcgacg gcgagatggc gggaagcgcc gacgcgaagg
    10981 agctcacccc cgagcgcgtc gtctccctga tggtggggcg acagatcgaa gccctctacc
    11041 cgcgaacctt ccgcgacccc ggcgccgtcc tcctcgacgt gcgcggactc tcgcgaagag
    11101 gggttctcga aggcgtctcg ttcacgctcc gcagcgacga aatcctcggc gtggcggggc
    11161 tcgtcggcag cggccgcacg gagctggcac gatgcctcgt cggtctcgac ccgttcgacg
    11221 cgggagagat cgccgtcgag ggaacgccgt tcaccccccg ctcccccggc gacgccctgg
    11281 acaggggcat cgtcctcgtc ccggaggacc gcaagcggca ggggctcttc ctcaaccgca
    11341 ccatccgcga caacacgatc tccatgcgcc tgctgcaagg gctccgcaag gggccgttcg
    11401 tcgaccgccc ggcctccgaa tcgctcgccg gatcgttcgt gcagcgcctc ggcgtgcggt
    11461 gcaggagcgt cttcgactcc gtcttctccc tcagcggcgg caaccagcag aaggtcgcca
    11521 tcgccaaggg gctcgccgtc tcgccgaaga tcctcatcct cgacgaaccc acgcgcggcg
    11581 tcgacgtcgg cgcgaaggcc gaaatccatc agatcatgaa cgacctcgcg gcgcagggga
    11641 tgggcatcct gatgatctcc tccgacctcc ccgaagtcct cggaatgagc gatgccgtcc
    11701 tcgtcttccg cgacggccgc tgcgtcgccc gcttcccgcg cggagagggc gcgcagggaa
    11761 ccctcggaga acgcgtcatg accgccgcga ctggaggaat cgccaatgcc tgagaaaaca
    11821 caatcgttcc ttgcaacgct gcgcgacaag ggctggggga gcgtcctgct gctcttcgcc
    11881 gtcgtgggcc tgctggccgc cttccacccc ggagtgctcg accggcagaa catggccaac
    11941 gtcctcgcgc agtccgccat cgtcggggtc tgcgccgtcg gcatgacctt cgtgctgctc
    12001 tcgggcggca tcgacctctc cgtcggcagc gttctcgccc tcgcaggagc cgtcggcgca
    12061 tggacctgca aggagctcgg cctccccgtc ccgctcgcgt gggccgtcgc cgtgctcacc
    12121 ggaaccctct gcggcctcgc ctccggttcg ctcgtcgcct tcgggcgcgt tccccccttc
    12181 atggccaccc tcgccatgct cgcggcggcg cgcggcggca cgctgctcct gacgcagggg
    12241 taccccgtct cgggactgcc tccggcgttc tacacccccg gatgggcctc ggtcgggccg
    12301 ttccccgttt caggactcgt cttcttcggc ctcgccctcg ccgcgtgggt gctgctgcag
    12361 cgcaccccct tcggcacgca cctccgcgcc ttcggagaca accccgagac ggcgcgcctc
    12421 gccgggctct ccgccccgcg cctcaccctc tccgtctacg ccatctccgg ctccctcgcc
    12481 ggactggccg gagtcctcat ggtcggacga ctctggagcg cccagcccaa cgtcggcatg
    12541 ggactcgaac tcgacgtcat cgccgcggtc gtcctcggcg gcgccagcct cttcggcggc
    12601 gtgggcggcg tcggcggaac cgtcgcgggc gtcctcatca tgggcttcct cgacaacggc
    12661 ctccgcctgc tggccatctc cagctacgta cagcagtcgg taaagggcct cgtcttcgtc
    12721 ggcgcggtgc tgctcgacct ggcgttcaag ggctactaca ggaaggggcg gggacgggat
    12781 gcgtgagggt ggattcgtgg cgtaaaaaaa acgactggac tcctcctctc ccttctcgct
    12841 ataatgctaa gaagttcttt tatctcgcat cgaagggggc ggcggtttgg agcagatggg
    12901 tttggga
//
LOCUS       DAOPKU010000014        12668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_5232_length_12668_cov_4.882581, whole genome shotgun sequence.
ACCESSION   DAOPKU010000014 DAOPKU010000000
VERSION     DAOPKU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12668
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5232_length_12668_cov_4.882581"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1644
                     /locus_tag="PK849_00925"
     CDS             <1..1644
                     /locus_tag="PK849_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPE64713.1"
                     /translation="TAAELTLPEASMIAGLIAAPGRYSPLSNFENAKNRQWYVLDRLV
                     TLGWISDEQRNEAYNAELVFRHTPNRVQEYNRAPYFVSHILFNDLLPKYGPDLVYSGG
                     MEIYTTLDLKMQEAAENAMKGLKSQGAIVGMDPETGEVLALVGGHDFAKSKFNRATQA
                     FRQPGSGFKPVVYATAIDSGLLPTDHFLDMKIEYAKKGPKQTTWGPGNYDGKYNGEVT
                     LQYALIRSLNTVAVRLIDYLGADPVVAMARRMGVVSQHLPADLSLALGTASVTPLEMA
                     AVFSVFANNGKAVDPLMIREIRSSNGDILETRRPYAREALSVEAAVTVRSMLLDAVRA
                     GTGRKALIPDRETFGKTGTTNDFTDAWFLGGVPGLVAVVYAGNDDHTPLGKRNVTGGD
                     VSAPVWNAFVSAAVKTVDLPKKFNIPSNAGVEAVSICRTTGFRALKGCSAATVYMRKG
                     AAPQTSCPIHGGDMNASTLDPNAPRLVLVHNDSELHTGDLYAAGMQPVETPPVAVTAQ
                     KQKQEVVVPESTGAYKDPNPAVTVEDRYQQLLKQYGLSN"
     gene            complement(1736..3157)
                     /locus_tag="PK849_00930"
     CDS             complement(1736..3157)
                     /locus_tag="PK849_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPE64714.1"
                     /translation="MTQNEKQTTEKTHSDLSGSVAQGKKLLCRGLAASPGSAVGRVVF
                     IPGTADISKVADGDIIVARMTTPDMVSALRKAGAVVTDEGGRTCHAAILSRELGIPCI
                     VGARNATKILSEGQLVLVDATRGVVFQADEAPEQEAAQPPRTPPVSKAFSPGSSLSAT
                     PPRAEQLPGVTATKLLLNIEGPAAIDRLASLPADGVGLLRTEFIFSGEIGTHPMHLVR
                     TKQGSLFVDKLAEGMSTVARAFFPRPVLARFSDFRTNDFRKLNGGQELEPVESNPMIG
                     WRGASRYISPDYEEGFRLECRAVKKVRDEMGNSNLSVIIPFVRTLKEMERVKAILASE
                     GLASGASFKIWLMAEVPSVVFQADAFASLVDGITIGTNDLTQLILGVDRDSAALENMG
                     YFDERNPAVLHAVSLVIEAARRAGIPCSVCGEGPSVFPDFAEHLVREGIDSISVNADA
                     LEYTRRIVASVEQKIILDALRGK"
     gene            complement(3242..5890)
                     /gene="ppdK"
                     /locus_tag="PK849_00935"
     CDS             complement(3242..5890)
                     /gene="ppdK"
                     /locus_tag="PK849_00935"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPE64715.1"
                     /translation="MTKEKYIYSFKEGNADMKALLGGKGANLAQMVTYGLPVPPGFIL
                     TTHACLEYGKNPGFLDSIWDGVVASMKTLEQEAGKTFGTGSNPLLVSVRSGAPISMPG
                     MMDTILNLGLNDESVESLAKAADDRRFAFDSYRRFIQMYGNVVHGVDGEAFEEVLTHT
                     KKRLGVTFDNEIPAKDLETIVAEFKKIFKEKAGFAFPSDPWDQLKNAILAVFKSWGNP
                     RAVTYRKLHNISDSLGTAVNIVSMVYGNLGDDCGTGVCFTRNPSDGTKELYGEFLINA
                     QGEDVVAGIRTPLPIAELKVKMPEIYKQLFKITTDLETAYKDMQDIEFTIERGKLHML
                     QTRNGKRSAGAAVKIAIDMLEEGLIDKKTAVSRVTPEQVEMLLHRQVDPKAEVTLLAK
                     GLPASPGAGVGIVTFDPDDAVDLVKAGNKVVLARPETCPDDIHGLFAAEAIVTSRGGM
                     TSHAAVVARGLGKPCVSGCEDLFIDLKNETITAGETVIRKGDFITVDGSTGRVLVGKA
                     PLIEASFTKDFERLLDWADEVSRLQVWANGDTPEDARRARAFGAKGIGLCRTEHMFMA
                     ADRLPVMQRLVVADSYEERVAALDELKVMQKGDFYGILKEMQGLPVIIRLLDPPLHEF
                     MPKERDLEEELHALVAAGKENSPEADKVRKVMAKVEMLKESNPMLGFRGCRLGIVYPE
                     ITAMQASAIFEAVIDLVKEGVDIHPEIMIPLVGVQKEMEFFRKQIDDIAKDYMEKSGV
                     KFSYLVGTMIEIPHAAFVADQIAEYAEFFSFGTNDLTQTTFGFSRDDAEGKFLGHYED
                     KGILPVNPFHVLDREGVGELMKLAVSKGRGRNPNLSIGICGEHGGNPSSIAFCHAVGL
                     NYVSCSPFRVPVARISAAHAAMGKLV"
     gene            complement(5929..6756)
                     /locus_tag="PK849_00940"
     CDS             complement(5929..6756)
                     /locus_tag="PK849_00940"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HPE64716.1"
                     /translation="MERVDTAPTCELFYLSDYLGEEMDSAASWITRRFEKIDIEISRY
                     GLLRTEGAVQKVLDEAARRDAFVLHGFLSGAFRRIVVERCAELGLDEYDVLRSLFSRL
                     SRIAGEEPRRDPSLVHPMDNEYFKRVKAIEYTIAADDGGNSSILKEADVVIIGVSRTG
                     KSPLCMYLAHKGIKAANIPLVPEIEPPVQLFQIEPARIIGLTRSSHSLEHIRTTRMHM
                     MGFDPDRSSYVKFERILSEIDYASGIMKRLGAVVFDVTARAIEETAHEILKLLRDGH"
     gene            complement(6782..7588)
                     /locus_tag="PK849_00945"
     CDS             complement(6782..7588)
                     /locus_tag="PK849_00945"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HPE64717.1"
                     /translation="MESLNLFIVSDSTGETAEHVALAAISQFNTDFANIYRYRYVEGE
                     ARIDEILAKASEAPSMVICTLVDKKSRSALAAKCAQHDTPFVDLLGPVIDALEIRLER
                     APLQAPGLLRKLDEDYFQRIKAVEFAIQCDDGRSPESLPEADLVILGVSRTGKTPLSM
                     YIANKGFRAANIPLIPEVDPPEILKTIPSSRIVGLIISGERLSQIRQERLRLLGLEPK
                     ASAYANRERIERELQTAREYLQSLGARIYDVTDRAVEETAQEILDFLRSK"
     gene            complement(7624..>8688)
                     /gene="glyS"
                     /locus_tag="PK849_00950"
     CDS             complement(7624..>8688)
                     /gene="glyS"
                     /locus_tag="PK849_00950"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPE64718.1"
                     /translation="EDLKKPLASNVERLKTIVYQEKLGTLHDKVMATRDLALWLCAET
                     GMKDVLQLLDRAALLAKADLVTNMVYEFPDLQGVMGREYALRNGEPERVARALYEQYL
                     PKAAGSELPEDIIGALLGLAERVHIITSCFKVGFEPTGSQDPYALRRAARCINEILWG
                     KAMDVNLGRLVARSCEQLAIQEDVRERIVAFLQQRLHVQIREKGYGHELSTLAVSVAG
                     SRPLQVLRFLEVFSKLQAEQWFVELVTSAVRVRNILAKSGDASASVDESLFAKEAESR
                     LYAEIVRINPLVEEALAGQDWKALAGYLSELSPFVTSFFEDVLVMDPDERVKCNRIHL
                     LGLCNALFLKVGDLGILKGV"
     assembly_gap    8689..8698
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8699..9697)
                     /gene="glyS"
                     /locus_tag="PK849_00955"
     CDS             complement(<8699..9697)
                     /gene="glyS"
                     /locus_tag="PK849_00955"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPE64719.1"
                     /translation="MTYKNVLLEIGTEEIPSRFLPDTLEFLEKTAKDDFKNARIGFKS
                     LSVFATPRRIALMVKDVDDQQQDLVNTFKGPAWTAAFDMNGNPTRAAFGFAKSKGVSI
                     EQLVGVEVDGVKYAHAEVSEPGKPVLEILPELLPALIKKIVFPKNMYWNIPTVRFARP
                     IRWIVAMADKNVIPFQYGGIESGSATKGHRFMGAKQIVVDDAANFMERLYDNHVILDQ
                     EKRRQKMIAGISALENDLDGAVELDPEVVNENLYLVEYPIPFFGSFSKKFLEIPEEVL
                     VTSMKKNQKYFSVRGKNGKLLPYFVGVSNNLASNMNVVREGNERVLRARLEDAAFFW"
     gene            complement(9703..10590)
                     /locus_tag="PK849_00960"
     CDS             complement(9703..10590)
                     /locus_tag="PK849_00960"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPE64720.1"
                     /translation="MNFQEIVFRLERFWAEQGCVVQQPYDIEVGAGTMNPATCLRVLG
                     PEPWRVAYVEPSRRPTDGRYGENPNRLQHYYQYQVIIKPAPSNIIDLYLQSLAALGIE
                     PEAHDIRFVEDDWESPTVGAWGLGWEVWLDGMEVTQFTYFQQVGGIDMDLVPAEITYG
                     IERIAMFVQKVENVYDLQWVDNVKYGDVHHKGEVEYSHYNFELADTKMLFQLFGMYEA
                     EADRVLAKGFVLPAYDYVLKCSHTFNLLDARNAISVTERTGYIGRIRMLASRCCAAYA
                     EQRREMGLPFTGKFDETKR"
     gene            complement(10649..11446)
                     /locus_tag="PK849_00965"
     CDS             complement(10649..11446)
                     /locus_tag="PK849_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPE64721.1"
                     /translation="MRAADIFVSSGGEYQDIPNRRSFPSYSGDSIPQGLVRLSGVVLR
                     RDVSAEGNVSLYLFLKETGPVWVSAPGASRGRVRFGGAIEPLVWANFNLYKGARRFYL
                     QSAEVKEDFWSLRSDSGKIRRMLEWDGLLCEHLVPGLPCDEVLALFYWSGVLLKEDVH
                     PLVAEWRFLWKWLYNWGIAPSPDLCGECGAALSSARLTASGFLCPACARTHEGTALSG
                     DELSLLRRSLEIPVKKMIEYFPSKPFPGDSPWKEVNGRLRLYFSMMQ"
     gene            complement(11452..12396)
                     /gene="era"
                     /locus_tag="PK849_00970"
     CDS             complement(11452..12396)
                     /gene="era"
                     /locus_tag="PK849_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPE64722.1"
                     /translation="MNNPFAGIGFHAGNVVLAGRPNVGKSSLVNRLLDYKVSIVSEKP
                     QTTRNVIRCVYNGEDFQIIFSDTPGIHLPKHKLGRTLVDAAVESLEEADLVCYVVEAE
                     DRTVAPEDEEILSVLGKVRTPVLLVVNKCDVVLDRKGGGEAALAAVRSIYEARISLCG
                     VVFVSARMGRGLDTLIAAIRPHLPEGPPFYDEDILVDRPEKFIAAEIIREKVLLMTHR
                     EVPHSVAVEIEEYKSPDEYPERDVLFIRATLYVEREGQKRIVIGDSGARLKEIGRRAR
                     MDIETLTGHKVYLDLWIKVRKDWRQSDADLRMLGIQER"
     gene            complement(12393..>12668)
                     /locus_tag="PK849_00975"
     CDS             complement(12393..>12668)
                     /locus_tag="PK849_00975"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPE64723.1"
                     /translation="NVENGSYGLSICAERSAAVAMIARGGKKPLAAAVVGKRGEVCLP
                     CGACRQFLAEFNPEMLLVFEQGDSFVVTTLAELFPAPFLLTEQSGAR"
BASE COUNT         2429 a         3629 c         3854 g         2746 t
ORIGIN      
        1 actgccgccg aactgactct ccccgaagcg tcgatgattg ctggtcttat tgcggctccg
       61 gggcgctaca gtcctctttc gaacttcgaa aatgcgaaga accggcagtg gtacgttctg
      121 gacaggcttg tgacgctcgg ctggatttcc gacgagcaac gcaacgaggc gtacaacgcg
      181 gaactggttt tccgccatac gccgaacagg gtgcaggagt ataaccgcgc gccgtacttc
      241 gtgtcgcata tcctgttcaa cgatctgctg cccaagtacg gtccggatct tgtgtattcg
      301 ggcggtatgg agatttatac caccctggat ctgaaaatgc aggaagcggc tgaaaatgcg
      361 atgaaggggc tgaagagcca gggagctatc gtcggtatgg acccggaaac gggcgaggtg
      421 ctggcgctgg ttggcgggca cgacttcgca aagagcaagt tcaacagggc gacgcaggcc
      481 ttccgtcagc cgggctccgg ctttaaaccg gtcgtctatg cgacggcgat tgattcgggg
      541 ctgctgccta cagatcattt tctcgatatg aagatcgagt acgcgaagaa ggggccgaag
      601 cagacaacat gggggccggg gaattacgac ggcaaataca acggcgaggt gacgcttcag
      661 tacgccttga tacgttcgct gaatacggtt gcggtgcgcc tgatcgatta tctgggggcg
      721 gaccccgtgg tcgctatggc gcgaaggatg ggggttgtat cgcagcatct tccggccgac
      781 ctttctctgg cccttggaac cgcgagcgta acgccgcttg agatggcggc tgttttttct
      841 gtgttcgcaa acaacgggaa agccgtagac cctctgatga tcagggaaat ccgctcttca
      901 aacggcgaca ttctggaaac gcgcaggccg tacgcgaggg aggctctttc agtagaggcg
      961 gcggtgacgg ttcgttcaat gctgctggat gccgttcgcg cggggacggg acggaaagcg
     1021 ctcattcccg accgcgagac gttcggcaag acgggaacaa cgaacgactt taccgacgcg
     1081 tggttcctcg gcggggttcc cggcctggtg gccgtggtgt acgcggggaa cgacgaccat
     1141 acgcccttgg ggaagcgcaa cgttaccggg ggagacgtct ccgctccggt gtggaacgcc
     1201 tttgtctcgg ccgcagtgaa gaccgtcgac cttccgaaaa aattcaatat cccttcgaat
     1261 gctggtgtcg aggcggtttc catctgccgc acgacaggct tccgcgctct taagggctgt
     1321 tcggcagcaa cggtgtacat gcggaagggc gccgcgccgc agacgtcatg cccgattcat
     1381 ggaggggaca tgaacgcgag tacgctggac ccgaacgctc ctcgcctggt gctggtgcac
     1441 aacgactccg agctgcatac cggggatctg tatgcagcag gaatgcagcc ggttgaaacc
     1501 cctccggtcg cggtgaccgc gcagaaacag aagcaggagg tcgtcgttcc tgagtcaaca
     1561 ggcgcgtaca aggatccgaa ccctgccgtg actgtggagg acagatacca gcagcttttg
     1621 aagcagtacg gcctgtccaa ctgagcatct tttccctcaa tgagcaaaga gcgccggaac
     1681 tcctgcattc acagggtccc ggcgctcctt ttctttgcgt tgcgcgtccc tgacgttact
     1741 tgccgcgaag ggcgtcgagg atgatctttt gttcgaccga tgcgacgatg cggcgagtgt
     1801 actccagtgc gtccgcattg acgctgatgc tgtcgatgcc ttcccgcaca aggtgttcgg
     1861 cgaagtcggg aaagactgag gggccttcgc cgcagacgga acacggtatt cccgcgcggc
     1921 gagcggcttc gatcacgagg gaaacggcgt gaagcaccgc aggattgcgt tcatcgaagt
     1981 accccatgtt ctccagggct gccgagtctc tgtcgacgcc gaggataagc tgcgtgaggt
     2041 cgtttgttcc gatggtgatg ccgtcgacca gtgaggcgaa ggcatctgcc tggaagacaa
     2101 cagagggtac ttccgccatg agccagatct tgaacgaagc gccggaagcg agcccttcgg
     2161 aggcgaggat ggccttgacc cgctccatct ccttgagggt gcggacaaag gggatgatga
     2221 ccgagaggtt ggagttgccc atttcatccc ggactttttt gacggccctg cattcgagcc
     2281 ggaagccctc ttcatagtcg ggggagatgt agcgggaggc gcctctccat ccgatcatcg
     2341 ggttggactc gaccggttcg agttcctgcc ctccgttgag cttccggaag tcgttggtgc
     2401 ggaagtcgct gaagcgggca aggacgggac gcgggaagaa cgcgcgcgca actgtgctca
     2461 tgccttccgc gagtttgtcg acaaagaggc tgccctgctt tgtgcgtacg aggtgcatcg
     2521 ggtgcgttcc gatctcgcct gaaaagatga attccgtacg gagaaggccg acgccgtccg
     2581 caggcagtga agccaagcgg tcgattgccg caggaccctc tatgttgaga agcagctttg
     2641 tcgcggtgac tcccgggagc tgttctgccc tgggaggcgt tgcggaaaga gagcttcccg
     2701 gtgaaaaagc ctttgaaaca gggggcgtcc gagggggctg cgccgcttcc tgttcgggcg
     2761 cttcgtccgc ctggaacacc acgcctcgcg tcgcgtcgac aaggacgagc tgcccctcgg
     2821 aaaggatttt cgtggcgttt ctggcgccga cgatacacgg aattccgagt tctctggaga
     2881 gaatggccgc gtggcaggtg cgcccccctt catcggtgac cacggctcct gcttttctga
     2941 gggctgagac catgtcgggg gtggtcattc tggcgacaat aatgtcgccg tcggcaactt
     3001 tggagatgtc tgcggtcccg ggtatgaaga ccactctccc gaccgcggat cccggagagg
     3061 cggcaaggcc ccggcagagg agcttcttgc cctgcgcgac gctgccggac aagtcggagt
     3121 gcgtcttttc cgttgtctgc ttctcgttct gtgtcatgct ctcagttcct ttccgatacc
     3181 cttgcgggca ggcgatagta cggcgccggg aggattcccg gcgccgcgtt cattgctgtt
     3241 gttataccag tttgcccatt gcagcgtgcg ccgcggagat acgcgcgaca ggcactctga
     3301 acggggagca gctcacatag ttcaggccga cggcatggca gaaggcgatg ctgctggggt
     3361 tgcctccgtg ctcgccgcag atgccgatgg agaggttcgg attccggcct cttcccttgg
     3421 agacggcgag cttcataagt tcgccgacgc cctctctgtc gaggacgtgg aacgggttga
     3481 cgggaagaat tcccttgtcc tcgtagtggc cgaggaattt gccctccgcg tcgtcgcggg
     3541 agaagccgaa agtggtctgt gtgaggtcgt tggtgccaaa gctaaagaac tcggcatact
     3601 cagcgatctg gtcggcgacg aacgccgcgt gcgggatctc gatcattgtg cccacgaggt
     3661 aggagaactt cacgccgctc ttctccatgt agtccttggc gatgtcgtcg atctgcttgc
     3721 ggaagaactc catctccttc tgaacgccga cgagcgggat catgatctcg gggtggatgt
     3781 cgacgccttc cttgacgagg tcgatgaccg cttcgaaaat ggcgctcgcc tgcatggcgg
     3841 tgatttcagg gtagacgatg ccgaggcggc agccgcggaa gccgagcatg gggttggact
     3901 ctttcagcat ttcgaccttc gccatgacct tgcggacctt gtcggcctcg ggcgagttct
     3961 ccttgccggc ggcgacgagg gcgtggagct cttcttcaag gtcgcgctcc ttcggcatga
     4021 actcgtggag gggcgggtca agaaggcgga tgataacggg cagcccctgc atctccttga
     4081 ggatgccgta gaaatcgccc ttctgcatga ccttgagctc gtcgagcgcg gcgacgcgct
     4141 cctcgtagct gtcggcgacg acaagacgct gcatgacggg cagccggtcg gcggccatga
     4201 acatgtgctc ggttctgcag aggccgatac cctttgcgcc gaacgcgcgg gcgcggcggg
     4261 catcctcggg ggtatccccg ttggcccaga cctgaaggcg ggagacttcg tcggcccagt
     4321 cgagcagtct ttcgaagtcc ttggtgaagg aggcttcgat gaggggcgcc ttgccgacaa
     4381 gcacgcggcc ggtgctgccg tcgacggtga tgaagtcgcc cttgcggata accgtctcgc
     4441 ctgcggtgat cgtctcgttt ttcaggtcga tgaagaggtc ttcgcagccg ctgacgcacg
     4501 gtttgccgag gccgcgggcg actacggcgg cgtggctggt cattccgccg cggctggtga
     4561 cgatggcttc tgcggcgaag agaccgtgga tgtcgtcggg gcacgtctcg ggacgggcaa
     4621 gaacaacttt gttgcccgcc ttgacaagat ccactgcgtc gtcgggatcg aaagtcacga
     4681 tgccgacgcc cgcgccgggt gatgctggaa ggcccttggc aagcaaggta acctcggctt
     4741 tgggatcgac ctggcggtgc agcagcatct caacctgctc gggggtgacg cgggagactg
     4801 ccgtcttctt gtcgatgagg ccctcttcga gcatatcgat ggcgatcttc accgccgcgc
     4861 ccgcggagcg cttcccgtta cgggtctgga gcatgtgaag cttgccgcgc tctatggtga
     4921 actcgatatc ctgcatgtcc ttgtaggcgg tctcgaggtc ggttgtgatt ttgaaaagct
     4981 gtttgtagat ctcgggcatc ttgactttca gctccgcaat gggaagcggg gtgcggatgc
     5041 ctgcgaccac gtcctcgccc tgtgcgttga tgaggaattc gccgtacagc tccttcgtac
     5101 cgtcggaggg gttgcgtgtg aagcacacgc cggtgccgca gtcgtcgccg aggtttccgt
     5161 agaccatgga gacgatgttg acggcggtgc cgaggctgtc ggagatgtta tgaagcttcc
     5221 ggtaggtcac cgcgcggggg ttgccccagc tcttgaagac ggcgaggatg gcgttcttca
     5281 gctgatccca ggggtcggac gggaaggcga atccggcctt ctccttgaag atcttcttga
     5341 actcggcgac gatggtttcg agatcctttg cgggaatttc attgtcgaac gtgacgccga
     5401 gccgcttttt cgtatgcgtc aggacttcct caaacgcctc tccgtcgacg ccgtgcacca
     5461 cgttgccgta catctggata aagcggcggt agctgtcgaa cgcgaaacgg cggtcgtcgg
     5521 cggctttggc aagcgattcg acggactcgt cgttcaggcc gagattgaga atggtgtcca
     5581 tcattccggg catcgaaatg ggcgcgccgg aacggaccga gacaagaaga ggattgcttc
     5641 ctgtgccgaa tgtttttcca gcctcctgct caagcgtttt catcgaggcg acgacaccat
     5701 cccaaatact gtcgaggaat ccgggatttt tgccgtattc gaggcatgcg tgcgtcgtca
     5761 gaataaagcc cggaggaacc gggagcccgt acgtcaccat ctgggcgagg ttggcccctt
     5821 tgcccccgag gagcgccttc atgtccgcgt tgccttcttt gaaactgtag atgtacttct
     5881 ctttcgtcat ctctatactg cacctccgta ggtttggata agatactttc agtgtccgtc
     5941 gcgaagcagc ttcagtattt cgtgcgccgt ctcctctata gctctggcag tgacgtcgaa
     6001 aacaaccgct ccaagacgtt tcattatacc cgatgcgtag tctatttcac tcaaaatccg
     6061 ttcgaatttt acgtaactgc ttctgtccgg atcgaacccc atcatgtgca tacgcgtcgt
     6121 ccggatatgt tccagagaat ggctcgaacg tgtcaggccg ataattcttg ccggctctat
     6181 ctgaaagagc tgaaccgggg gctcgatttc agggacaagc ggtatgttgg cggctttaat
     6241 tcccttgtgg gcaaggtaca tgcacagagg agacttcccg gttctggaaa cgccgatgat
     6301 gacgacgtcg gcttctttca ggatcgacga gttcccgccg tcgtcggcgg caatagtgta
     6361 ctcgatcgcc tttacccgct tgaaatactc attgtccatc gggtgtacca gggacggatc
     6421 gcgcctgggc tcttctccgg cgattcgcga gaggcgggag aacagagacc ggagcacatc
     6481 gtattcgtca agtccgagtt cggcgcacct ttcgacaaca atgcggcgaa aagcgccgga
     6541 gaggaatccg tgaagcacga aggcgtctct tcgggcagct tcgtcgagca ctttctgcac
     6601 ggcgccttcg gtacgaagaa gaccgtatcg ggatatttcg atatcgatct tttcaaagcg
     6661 cctcgtaatc cacgacgcgg ccgaatccat ctcctctcct aaatagtcgg agaggtagaa
     6721 aagttcgcac gtcggcgcgg tatcgacacg ttccatactg ccggaaaggt cttctctcaa
     6781 tctatttcga acgcaggaaa tcgaggatct cctgcgcggt ctcttcgacg gccctgtcgg
     6841 taacgtcgta aatgcgggcg ccgagcgact ggagatactc cctggcagtt tgaagttctc
     6901 tttcaatccg ctcccggttt gcataggccg acgccttcgg ttccaatccc agaaggcgaa
     6961 gacgctcctg acggatctgt gagagccttt ctccggagat gatcagccct acaatcctcg
     7021 atgacgggat ggttttaaga atctcaggcg gatcgacttc cggtatgagg gggatattcg
     7081 ccgccctgaa tcctttgttc gcgatgtaca tcgagagcgg cgtcttcccg gtacgggaga
     7141 ctccaaggat gaccaggtcc gcctccggca gcgattcggg gctgcgtccg tcgtcgcatt
     7201 ggatggcgaa ttccacggcc ttgatccgct gaaaatagtc ctcgtcgagc tttctgagaa
     7261 gtcccggcgc ctggagcgga gctctttcga ggcgaatttc gagcgcatcg atgacaggcc
     7321 ccagcagatc aacgaaaggg gtgtcgtgtt gggcgcactt ggcggcgagc gcggagcggc
     7381 tctttttgtc caccagcgta cagatcacca tggagggagc ttccgacgcc tttgcgagta
     7441 tttcgtcgat tctggcctcc ccttcgacat accggtaacg gtaaatgttg gcaaaatctg
     7501 tattgaattg actgatggcc gccagagcca cgtgctccgc cgtctcgcca gtggaatcgg
     7561 atacgatgaa caaattcaag gattccattg cttcacactc cttatgttga cgccgctctc
     7621 aggctacacg cccttgagta ttcccaggtc cccgaccttc aggaagaggg cgttgcacag
     7681 acctagaaga tgtatcctgt tgcattttac cctttcatcg gggtccatta caaggacgtc
     7741 ttcgaaaaat gacgtaacaa atggcgaaag ttccgacaga tacccggcaa gcgccttcca
     7801 atcctgcccg gcgagcgctt cttcgacgag gggattgatt ctgacgatct cggcgtagag
     7861 cctgctctcg gcctcctttg cgaaaaggct ttcgtcgacg gatgcggagg cgtcgccgct
     7921 cttggcgagg atgttgcgga ctctgacagc cgatgtaacg agctcgacga accactgctc
     7981 cgcctgaagc ttggagaaga cttcgaggaa gcgcagcacc tggagcggac ggcttcccgc
     8041 cactgaaacg gcgagcgtgg aaagctcgtg gccgtacccc ttctcgcgaa tctgcacgtg
     8101 cagccgctgc tgtaagaagg cgacgatcct ttcgcggaca tcctcctgga ttgctaactg
     8161 ctcgcagctt ctggccacca gccgcccgag attgacatcc attgcctttc cccagaggat
     8221 ctcattgatg cagcgggccg cccttcgaag ggcgtacgga tcctgagatc ctgtaggttc
     8281 gaacccgacc ttgaagcagc ttgtgataat gtgcacccgt tcggccagtc cgagaagggc
     8341 gccgatgata tcttcaggca gctcgctccc cgcggccttg ggaagatact gttcgtagag
     8401 cgccctggcc acccgttcag gctctccgtt gcggagggcg tactctcttc ccatgactcc
     8461 ctggaggtcg gggaactcat agaccatgtt tgtcacaagg tcggcctttg caagaagagc
     8521 ggcccggtcc agaagctgga gcacgtcttt catccccgtc tccgcgcaga gccagagcgc
     8581 aagatcgcgg gtcgccatca ccttgtcgtg gagagtgccg agtttttcct ggtagacgat
     8641 tgttttcagc cgctcgacgt tggatgcgag gggctttttc agatcctcnn nnnnnnnncc
     8701 aaaagaacgc ggcgtcctcg aggcgtgcgc gaaggacccg ctcgtttccc tcgcggacga
     8761 cgttcatatt cgatgcgaga ttgttgctta ccccgacgaa atagggaagg agctttccgt
     8821 tctttccccg cacagagaag tacttctggt tcttcttcat cgaggtgacg agcacttcct
     8881 ccggtatttc gaggaacttc ttgctgaagc ttccgaagaa gggaatcggg tattcgacca
     8941 gatagagatt ctcgttgaca acctccggat cgagctcgac agcaccgtca aggtcgtttt
     9001 cgagcgcgct gatacctgcg atcatcttct gacggcgttt ttcctggtcg agaatcacgt
     9061 ggttgtcgta gaggcgctcc ataaagttcg ccgcgtcgtc caccacgatc tgctttgcgc
     9121 ccatgaagcg gtgtcccttg gtcgctgaac cgctctcgat tcctccgtac tgaaacggga
     9181 tgacgttttt atcggccatt gccacaatcc atcggatggg gcgcgcaaag cggacggtgg
     9241 gtatgttcca gtacatattc tttggaaaga cgattttttt aatgagcgcc ggaagaagtt
     9301 cgggaaggat ttccagcacc ggctttccgg gttccgaaac ttcggcgtgg gcgtacttga
     9361 cgccgtccac ctcgacgccg acaagctgtt cgatggagac gcccttgctc ttggcaaagc
     9421 cgaaggccgc gcgcgtcggg tttccgttca tgtcgaacgc ggctgtccat gccggtcctt
     9481 tgaacgtgtt caccaggtcc tgctgctgat cgtcgacgtc tttcaccatt aaggcaattc
     9541 ggcgcggcgt cgcaaagacg gagagactct tgaagcctat gcgggcgttc ttgaagtcgt
     9601 ccttcgccgt tttttcgagg aattcgagcg tatcggggag gaaacgtgag ggaatttcct
     9661 cggtcccgat ttcgagcagt acgtttttat aggtcatggt cctcacctct tcgtctcgtc
     9721 gaatttgccg gtgaagggaa gccccatctc ccggcgctgc tcggcgtagg ccgcgcagca
     9781 tctgcttgcg agcatgcgta ttcttccgat gtaccccgtc ctctcggtca cgctgatcgc
     9841 gttgcgcgcg tcgagcaggt tgaaagtgtg cgaacacttg agcacatagt cgtacgcggg
     9901 aaggacgaat ccttttgcaa gcaccctgtc ggcttcggcc tcgtacatgc cgaagagctg
     9961 gaagagcatc ttcgtgtcgg caagttcgaa gttgtagtgc gagtactcga cctcgccctt
    10021 atggtggacg tcgccgtact tcacgttgtc cacccactgg agatcgtaga cgttttcgac
    10081 cttctggacg aacatggcga tgcgttcgat cccgtaggtt atctccgcgg ggacaaggtc
    10141 catgtcgatg ccgccgacct gctggaagta cgtgaactgc gtaacctcca tgccgtcgag
    10201 ccagacctcc cacccgagcc cccacgcgcc gacggtgggc gactcccagt cgtcctcgac
    10261 aaaccggata tcgtgcgcct ccggttctat cccgagagcc gcgaggctct gaaggtagag
    10321 atcgatgatg ttggaggggg cgggcttgat gatcacctga tactggtagt agtgctgaag
    10381 ccggttcggg ttctctccgt agcggccgtc ggtcggacgg cgcgagggtt cgacgtaggc
    10441 gacgcgccac ggttcgggcc cgaggacgcg aaggcaggtc gcgggattca tagtcccggc
    10501 cccgacctct atgtcgtacg gctgctgtac aacgcacccc tgctccgccc agaagcgttc
    10561 gaggcgaaag acaatttcct ggaaattcac atatatccct ccttgagaaa aacttccggc
    10621 tgccggaagc tctattcttt ttccactctt attgcatcat actaaagtac aggcgaagac
    10681 gcccgttcac ttccttccag ggcgagtctc ccggaaacgg tttcgaggga aaatattcta
    10741 tcatcttttt caccggaatt tccagcgacc gacgcagcaa ggagagttca tccccggaaa
    10801 gcgccgtccc ttcgtgcgta cgggcgcagg ccgggcagag aaatccggaa gccgtcagac
    10861 gggctgaaga gagcgcggcg ccgcactcgc cgcaaagatc gggtgaaggc gctatgcccc
    10921 aattgtacaa ccacttccac aggaagcgcc actccgcgac aagcggatgg acgtcttctt
    10981 tcagcagcac gcccgaccag taaaagagcg caagcacctc gtcgcagggg agtccgggta
    11041 cgaggtgttc gcagagcagt ccgtcccatt cgagcatcct ccggattttt ccggaatcgg
    11101 acctcaggct ccagaagtcc tcttttacct ccgcggactg aaggtagaag cggcgcgccc
    11161 ccttgtacag gttgaaattc gcccagacga gcggctcgat ggcgccgccg aaacggactc
    11221 tccctctcga cgctcccggc gcagagaccc agaccggccc cgtctccttc agaaagagat
    11281 agagagagac gtttccctct gcggagacgt ccctgcggag cacaaccccg gacagtctga
    11341 cgagcccctg ggggatggaa tcgccgctat aggacgggaa ggaccggcgg ttcggaatgt
    11401 cctggtactc cccgccggac gaaacgaaaa tatccgctgc acgcaccccc gtcacctttc
    11461 ctgaatgccg agcatccgca gatcggcgtc ggattgacgc cagtctttgc gcaccttgat
    11521 ccagaggtca agatagacct tgtggccggt gagcgtttca atatccatcc gcgcccgtct
    11581 gcctatctcc ttcagccgcg ctccggaatc gccgatgacg atgcgcttct gcccttcgcg
    11641 ctccacgtag agcgtggcgc gaataaaaag cacgtcgcgc tcggggtatt cgtcagggct
    11701 tttgtactcc tctatttcga cggcgacgct gtgcggcacc tcccggtgcg tcatcagcag
    11761 cactttttcc cgtatgatct ccgcggcgat gaacttctcg ggccggtcga caaggatatc
    11821 ctcgtcgtaa aaaggaggtc cttcggggag gtggggacgg attgcggcga ttagcgtatc
    11881 aagtcctctt cccattctgg cggagacgaa gacgaccccg cagagagaga ttcgcgcctc
    11941 gtagatggag cgcacggcgg caagcgccgc ctcccctcct ccttttcggt cgagaacgac
    12001 gtcgcacttg ttcacgacga ggagcacggg ggtgcgcacc tttccgagga cggagaggat
    12061 ttcttcgtcc tcgggggcga cggttctgtc ctccgcctcg acgacgtaac agacgaggtc
    12121 ggcctcctcc agcgattcta cggctgcatc caccagcgtt ctgccaagct tgtgcttcgg
    12181 gagatgaatc cccggagtgt cggagaagat gatttgaaag tcttccccgt tgtagacgca
    12241 acggatgacg tttctcgttg tctgcggttt ctcggaaacg atggaaacct tgtagtcaag
    12301 aagcctgttg accagcgagg acttcccgac gttgggacgg cccgcgagga cgacgttccc
    12361 cgcatggaag ccgatccctg cgaaggggtt gttcatcgtg ccccgctctg ctccgtcaga
    12421 aggaacgggg ccggaaagag ctctgccaat gtggtcacga cgaatgaatc tccctgttca
    12481 aagacaagca gcatttccgg gttgaactcc gcgaggaact gccggcacgc gccgcacggc
    12541 aggcacactt ctcccctttt tccgacaacg gctgcggcaa gaggcttttt gcctccgcgg
    12601 gcaatcatcg cgacggcggc gctccgctcg gcgcagatgg agagtccgta ggagccgttc
    12661 tccacgtt
//
LOCUS       DAOPKU010000015        12370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_5373_length_12370_cov_4.921721, whole genome shotgun sequence.
ACCESSION   DAOPKU010000015 DAOPKU010000000
VERSION     DAOPKU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12370
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5373_length_12370_cov_4.921721"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PK849_00980"
     CDS             <1..1222
                     /locus_tag="PK849_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE64724.1"
                     /translation="EMALLNRLTGRLLAIEGALEMAETLCSEIEAVFSPAFIRFHLIA
                     GGEKRKLLFGEKNELSMDPLEEDFLDWVVENRKGVASPASDVARLWSSGGSDSPVLHS
                     SIFSAVSRGDVFLPALSSSGIEGILHVGERRSRSPYRWNEVQLLFAAARMAGTFLERS
                     RLGESARQARTLKEGDKLKSAIFSSVSHELKTPLSSLTATISGLLEQDAEPDPSRVRQ
                     ELLSVKKDLDRLSHSIGSLLELSRLAAEAWKPDLQWAEPGEVLSWVLFRLDKTDRERV
                     RVDLPEHLPLILVDFQQWGRLLMHLLENALSYSPAGSPVAVGARSTDEGFVTWIADEG
                     PGIPDEEKSRVFEKFFRGTFSNRLPGGTGLGLPIAMEIARVHEGSISIEDNSPAGTRI
                     VVRLPAAHVMETEE"
     gene            1227..1928
                     /locus_tag="PK849_00985"
     CDS             1227..1928
                     /locus_tag="PK849_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE64725.1"
                     /translation="MTSAPERNIRVLVIDDEESIRRALKSILSARKYEVSLAATAAEG
                     IDTAIETNPELVVLDLALPDMDGQDVCKELRAWMTAPILILSVRGNETDKIQALDAGA
                     DDYLTKPFQAGELLARMRALLRRAESRVVSPPELCAGDLTVNLARRQVFVKGEEVSLT
                     RTEFEILALLVQNADCVVTHRMLMEKLWDDKEERDPRLLRVHVSNLRKKIEPAPSLPR
                     FIHTEPGVGFRFTPE"
     gene            2049..2531
                     /locus_tag="PK849_00990"
     CDS             2049..2531
                     /locus_tag="PK849_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64726.1"
                     /translation="MTAQDIGLCSVIEKLRRNGAEADIAEAAQYMHRNEITLLMLKRA
                     GVPLEIMDACFLLSQCADSLIPERVEELRRSSLARTIKIGELRALLQEGDMRAAFRDR
                     WEICRESLVHALDILENGARADESPKKKPTMALFWERFAEFYEETEEMQNVALSGRSS
                     "
     gene            2503..2691
                     /locus_tag="PK849_00995"
     CDS             2503..2691
                     /locus_tag="PK849_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64727.1"
                     /translation="MLLCLGDLLEANASCSLEENGELHLYFQDESDILYGEDNKVVVS
                     AGESDLSGSDAVKWLQKH"
     gene            2831..4093
                     /locus_tag="PK849_01000"
     CDS             2831..4093
                     /locus_tag="PK849_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPE64728.1"
                     /translation="MIGAGTLLLLLFNIHSHPLRFVDALFTATSAVCVTGLVVVDTGK
                     DLAPASQTVVLLLIQLGGIGVMTATTALSLLIRERIGLRQRMFLAGGFGMETPAGVVR
                     FLRRILLLTFSVETVGALFLYAGFLRAGFSPLRSLYNAVFHSVSAFCNAGFSLFSDSL
                     AGFSRNLLIPGTVMALIVVGGLGFMVLSELMMTVRRREKLSMLPRLVLFTTAGLLLFG
                     LFSITLSEWNHAFAGMSGGWKIWNGLFASVTPRTAGFDTVPPATFSSAGIFLLILLMI
                     VGASPGSTGGGFKTTTLAVLLVSTWNNLRKNPEINLWNRRISIRILQRTVTVVVLYLG
                     TIGLGLGALALLEPFPFRSLLFETVSAMGTVGLSLGITAELSDAGKIVLMLLMFWGRV
                     GIVTFLYGLLKPVPRGKVSYPDADIPIG"
     gene            4106..4765
                     /locus_tag="PK849_01005"
     CDS             4106..4765
                     /locus_tag="PK849_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPE64729.1"
                     /translation="MNDKEKVVLVAGLGRFGQSLCKTLSEQGRQVIAVDRSRQFVEEM
                     ADIVDLAVQADVTDSEALVKVGAKEADIGIVAIGGNIEASILATTILKDLEIPYVIAR
                     AQNTVHARVLARVGANRVVFPERDMGERLALLLVHPWMSHFVQVPGSRFHVGEIRPLP
                     EMVGKTLPELNFRVRYNAVVLSVNRSGDRFLPTAETVIEAGDMLLVAGRREDLEKWVE
                     E"
     gene            5002..5868
                     /locus_tag="PK849_01010"
     CDS             5002..5868
                     /locus_tag="PK849_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64730.1"
                     /translation="MTSTALRPEDTSASAGALLFYYPAAFLLFFADILIVDFPGIRIG
                     MLDVLPAVPGITWSCVGAVFAVLMLVATVRYVAGGGTSDLARLFLRAGHSVVVLLSFI
                     GFLVVPMAVTLSGLSESTDTYGAFVAFRGAFFFLFLFVLSGMSHAFLATGRTDRGTTI
                     VGASQKLLAAQMAMLSAAFLFLVLSAPGTLPSLSCSMDVPSWLVYLLPVCFVLVGALF
                     ASFGGGAEHGALLGFMFRLCVLLSAAAPVAVLLAGRSEESGMALYMASLALFVSALKG
                     LYAVEASEEAAA"
     gene            6026..6622
                     /locus_tag="PK849_01015"
     CDS             6026..6622
                     /locus_tag="PK849_01015"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPE64731.1"
                     /translation="MQKQDTPFPVLFTPRLRLCAIESSHAERLHAVWTDPLVIEYLVL
                     DPFTELRQSEEMIGLLQWLRPSGEGVRWAITLRSDGTVLGTCGFHNWKKEHGRAEMGY
                     ELDSRMWRQGFMSEAVFAALEYGFGTMELNRVEALVTEGNQRSRGFLEKAGFRLEGTL
                     RQYEWARGRYQDQWMFSLLKEDRKGGQFTSVIDTPASL"
     gene            6709..7755
                     /locus_tag="PK849_01020"
     CDS             6709..7755
                     /locus_tag="PK849_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPE64732.1"
                     /translation="MNRTATMKDVARKAGVSTATVSHVLNGTRPVGSVLRDRVRQAVA
                     ELGYRPNFIARSLRRKGSGAVGVILIDDTNPFFSESTRGVKDAALENGYSVLVCNSEG
                     DPDRENEFLELLAEKQVEGVILFNVRDSNLREAAGRRLAVPVVLVDRDGASRGMDSVV
                     VDHYAGGMDAMEHLLSLGHGRIACIAGPSNSTPALLRLRAYANALRRAGIPYDKSLVY
                     RGDFRSESGYAAIPFLFSDGKLPTALFAFNDLMAFGAITALAEMGLRIPEDVSVVGYD
                     DILLSSFFNPPLTTVAQPKKEMGRAAFGLLLDRINDESLPARSLRMNARLVVRKSTAS
                     PGASKGGDGSGCMR"
     gene            7895..8932
                     /locus_tag="PK849_01025"
     CDS             7895..8932
                     /locus_tag="PK849_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE64733.1"
                     /translation="MKGLRKVLLLVMVLVIALSAFGVAGAAESKVKVALVLSGFLGDK
                     SFNDSAYEGLERSIKEFGIDLKVLESKVPSDWESNLVAMAAEGYDLVLGCSTQLQEII
                     AKHAPDFPDVKFGIIDGVVKAPNVMSAIFAQNEGSFLAGAAAAMFTTKTDVPGVNAEK
                     IIGWVGGMDIPVLHDFLVGYKQGAAYVDPETRVLVSFAGTFNDPLKGKELTLAQFEQG
                     ADIVMNVASNTGNGILEAAYEAKKYAIGVDLNQDEAYPGHILTSMLKRVDMGSYLIVK
                     AVVEGNFEGGKILDMTIANGGVGLTDMSTMKKALGDKFPQDILESIAKLTEEVKGGKI
                     KVDSYEGFQRN"
     gene            8995..10512
                     /locus_tag="PK849_01030"
     CDS             8995..10512
                     /locus_tag="PK849_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64734.1"
                     /translation="MSRILIEMKGICKEFPGVKAVQDGLFSLEEGEIHSLIGENGAGK
                     STLMKILYGMYVPEEGSIAVKGETYPSLTPKQAIGLGIGMVHQEFMLVREMTVLENII
                     LGFEPRRGGRIDRRAALEKIEYYADTYGLRVHPDKKVEEISVGEAQRVEIIKTLYRGA
                     EILVLDEPTAVLTPQEAEQLFCILRSMKEGGKSIVFISHKLQEVLAISDRITVMRGGK
                     HIATVAGSSTSAPELARLMVGREVFLGGSPVPAAKGEVALSVENVFVPGEKELSKLRG
                     VSFSVRKGEILGIAGVNGNGQSELAEAIAGLRQVQRGRVVLGGAEVQNMPPRRIREHG
                     LSHIPEDRNQRGLDRFSDVKENLAACSVSEPPLSSLGVIRWGKVREFAEGLIRSYDIR
                     PAGADIEASGLSGGNAQKVVVAREVSRKAEVLLASQPTRGVDIGSIETIRNELNRAKE
                     AGAAGVLISSDLEEILSLSDRIAVMFEGRITGELDSSEADEEKLGLLMTGGGTHV"
     gene            10505..11545
                     /locus_tag="PK849_01035"
     CDS             10505..11545
                     /locus_tag="PK849_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64735.1"
                     /translation="MYKTSWGSTLMTIILSLALGAGAILLLGYDPIEAYYQLFRGAFV
                     GKFNIGGVMERFVPLMLTALAFAVSAKVAVFNVGVEGELYLGAIAAAWAGYAFTGLHP
                     LVHIPLCFAVAMVASGAWGAIPGALKAYWKVNEVCVTILMNYVAIYFTSYLVGNPFSA
                     RASIPRTPTIEESARLLRILRPSRANIGIFVALGVLVLVWWMLYHTNWGYKLRHVGQN
                     PSFSEYVGIPPKKVMIWGMILSGAIGGLAGAIEVMGIHGHFLDNFSPGIAFDGMLASL
                     IAKNDIRMVPVLSLFLAVLKTGALGMERFTGVPKTLIDTIIAVFILLAAMEGLFAYSR
                     RRSARSSGEEAA"
     gene            11545..>12370
                     /locus_tag="PK849_01040"
     CDS             11545..>12370
                     /locus_tag="PK849_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64736.1"
                     /translation="MDGLNEILSLVFNATLVRSTIRAATPILYATLACVLTQQANILN
                     IGIEGIMIFAAFAAVTTSYFAGSWVLGLLAAIVTGVVVAAFMAFAHLKAKADIFVVGM
                     GVNMLAIAMTRFLLQRTFGVSGSFHHNDIVPMPRLNFDFGGNALLDTLFNDYSLFEPV
                     GIVLVFVLAFILYKTVWGLRLRSVGLNPLAAETAGINVTFRKFEVMLYSGVLGGIAGA
                     HLSLGYSRMFVENMTNGRGFMGVAAMFFGGANPFWGWIGCLIFGFADSLGSRLQSLG"
BASE COUNT         2251 a         3656 c         3862 g         2601 t
ORIGIN      
        1 ggagatggcc ctcctcaaca ggctgaccgg gaggctgctc gccatcgaag gcgcccttga
       61 gatggcggag acgctctgct ccgagataga ggctgttttc tccccggcct tcatacgttt
      121 ccacctgatc gcaggcggtg agaaacgaaa gctcctgttc ggagagaaga acgagctttc
      181 gatggaccct cttgaggagg attttctcga ctgggtggtc gaaaaccgga aaggggtggc
      241 gtctcctgcg tcggatgtcg cccgcttgtg gagttcgggc gggagcgaca gcccggtgtt
      301 gcattcgtcg attttctccg ccgtgtcgag aggggatgtc tttctcccgg cgctctcttc
      361 ttcaggaatc gagggcatac ttcacgtcgg ggaacgtcgc agccggagtc cgtaccgctg
      421 gaatgaagtg cagcttctct tcgccgccgc gcgcatggcc gggaccttcc ttgaacggag
      481 ccgcctcggc gagtcggcgc ggcaggcccg gaccctgaag gagggagaca aattgaagtc
      541 ggcgatattt tcgtcggttt cgcacgagtt gaagacgccg ctctcctcgc tgacggccac
      601 gatctcggga cttctggagc aggatgccga accggaccct tcgcgcgtac gccaggagct
      661 gctctcggtc aaaaaggatc tcgacaggct ctcgcactcc ataggctccc tgctggaact
      721 ttctcgtctt gccgccgagg cgtggaagcc ggatctgcaa tgggcggagc ccggcgaggt
      781 gctgagctgg gtgctcttca gactcgacaa gacggatcgc gagcgcgtac gggtcgacct
      841 cccggaacat ttgccgctta tccttgtcga ttttcagcag tggggaaggc tgctgatgca
      901 cctgctggag aacgcgctct cctacagtcc cgccggaagt cccgtggccg tgggcgcccg
      961 cagcacggac gaagggttcg tcacgtggat tgcggacgag ggacccggaa taccggacga
     1021 ggagaagagc cgggtcttcg agaagttctt ccggggaacg ttttcgaacc gccttcccgg
     1081 cggaacgggg ctcggtctcc ctatagcgat ggagattgcc agggtccacg aaggaagcat
     1141 cagtatcgag gacaactcgc ccgcgggaac ccgtatcgtc gtccggctcc ccgcggcgca
     1201 cgttatggaa acggaggaat gaacggatga cgagcgcgcc cgaaagaaat atcagggttc
     1261 tggtgatcga cgatgaagag tccatcaggc gggcgctcaa gtcgattctc tccgccagaa
     1321 agtacgaggt gagcctcgcg gcaaccgccg ccgaagggat cgacacggcc atcgagacga
     1381 accccgagtt ggtcgtcctc gaccttgcgc tcccggatat ggacggacag gatgtgtgca
     1441 aggagctgcg cgcctggatg accgctccca tcctcatcct ttcagtgcgg ggaaacgaaa
     1501 ccgacaagat acaggccctg gacgcgggcg ccgacgacta cctgaccaag ccgttccagg
     1561 ccggcgagct tctggcgagg atgcgggcgc tgttgcggag ggctgagtcg agggtcgtgt
     1621 cccctcctga actctgcgcc ggagatctga ccgtgaatct cgccaggagg caggtcttcg
     1681 tgaaggggga ggaggtttcc ctgacgcgca cggagttcga aatactggcg cttcttgtcc
     1741 agaacgccga ctgcgttgtt acccaccgta tgctgatgga gaagctttgg gacgacaaag
     1801 aagagcgcga cccgcggctt cttcgtgtgc atgtgagcaa cctgagaaaa aagatcgagc
     1861 ccgccccatc cttgccgcgc ttcattcaca ccgagcccgg agtaggattc cgcttcaccc
     1921 ccgaatgatc ttcacccttt acgaaaactt tacgcccgcg ccccgtttct tgacggagcc
     1981 tttatccctc gcttggtagc atatgcctgc acatggaaag aaatgccttt tcaacaggag
     2041 gattctttgt gactgctcaa gacatcgggt tgtgctccgt gatagaaaag ttgcggcgga
     2101 acggagcgga ggcggatatc gccgaggccg cgcagtacat gcatcggaac gagatcaccc
     2161 tgctcatgct gaagagagcg ggagtgccgc tcgaaatcat ggatgcatgt tttctgctga
     2221 gtcaatgcgc ggattcgctg attccggagc gggtcgaaga gttacgccgc agctccctgg
     2281 cacgaacaat caagatcgga gaactccgag ccttgcttca agaaggggat atgcgcgctg
     2341 ccttccggga tcggtgggaa atatgcaggg aatcactcgt gcatgcgctg gatatactgg
     2401 agaacggcgc cagggcggac gaatccccga agaaaaaacc cacgatggcg ctgttttggg
     2461 agcggttcgc ggagttctac gaggaaacgg aggagatgca gaatgttgct ctgtctgggc
     2521 gatcttcttg aggcgaacgc ctcgtgttcg ctcgaggaaa acggcgaact gcatttgtac
     2581 tttcaggatg agtccgatat actgtacggc gaggacaaca aggtggtcgt atccgccggg
     2641 gagagcgatc tttccgggag cgacgccgtg aagtggttgc aaaaacactg attttgcgcg
     2701 tgcataagaa agaaaccgcg aagagagaaa tgcccgggaa gacgcacgtc cgggaggagg
     2761 tcgtagtata gcattcagag cctttatctc gctggccatt gaaaggaaac tcgtcgcagg
     2821 gttcctgctc ctgattggtg caggaactct gttattgctt cttttcaata tacattcgca
     2881 tcctctgcga tttgtggacg cgctctttac agctacttcg gcggtctgcg tgacgggact
     2941 cgtcgtcgtg gatacgggca aggacttggc gccggcctct cagaccgtcg tcctgctgct
     3001 catacagctt ggcggtatcg gtgtgatgac ggcgacgacg gcgctctccc tgctgatccg
     3061 ggagagaatc ggactacgac agcggatgtt cctcgccggt ggttttggta tggagacgcc
     3121 tgcgggcgtg gtgcgtttcc tgcggcggat tttgctgcta accttctccg tcgagactgt
     3181 cggggcgctc tttctctacg cggggtttct ccgtgccgga ttttccccgt tgcgttcgct
     3241 gtacaacgcc gtgtttcaca gtgtcagtgc cttctgcaac gccggcttct ccctcttctc
     3301 cgacagtctg gccggttttt cgcggaatct tctgattccg gggacggtca tggcgcttat
     3361 cgtcgtcggc ggactggggt tcatggtgct ttccgagctc atgatgacag ttcgcagaag
     3421 ggaaaaactt tcgatgctcc cgcgtcttgt gctctttacg acggcggggc ttttgctttt
     3481 cggccttttc tcaatcaccc tctcggagtg gaatcatgcg ttcgcaggga tgtccggcgg
     3541 atggaagatc tggaacggac tgttcgcgag cgtcacgccg aggaccgcgg gcttcgatac
     3601 ggtccctccg gcgaccttct cctccgccgg tatcttcctt ttgatactgc tgatgatcgt
     3661 cggcgcctcc cccggctcca caggaggcgg attcaagacg acgacgcttg cagtgctgct
     3721 ggtctcgacg tggaacaacc tcaggaaaaa ccccgagatc aacctgtgga acagacgaat
     3781 ctccatccgg atactgcaga ggaccgtcac ggtcgtcgtc ctctatctgg ggaccatcgg
     3841 attgggcctg ggggcgcttg cgctgctcga accctttccc ttcaggtcgc tgctcttcga
     3901 gacagtctcg gcgatgggaa cggtcggcct ttcccttgga atcactgcgg agctgagcga
     3961 cgcgggcaag atcgtgctca tgctcctgat gttctggggg cgcgtcggca tcgttacctt
     4021 cctctacggg cttctgaaac ccgtgccgag aggaaaggta tcctaccccg atgcggacat
     4081 accaataggt taggaggtgc gggaagtgaa cgataaagag aaagtcgtcc tggtcgcagg
     4141 gctgggacgt tttggtcagt cgctctgcaa gacgctttcc gagcagggga gacaggtgat
     4201 cgcggtggac cgttcccggc agttcgtcga ggagatggcg gacatcgtcg accttgccgt
     4261 gcaggcggac gttacggatt ccgaggcgtt ggtgaaggtc ggcgcgaagg aggctgacat
     4321 cggcattgtg gcgatcggcg gcaacatcga ggccagtatc ctcgcgacga ccatactgaa
     4381 ggatctggag atcccgtacg tcatcgcccg ggcgcagaat acagtgcatg cgcgtgtttt
     4441 ggcccgcgtg ggggcgaacc gcgtcgtctt ccccgagcgg gatatggggg agcggctggc
     4501 gttgctgctg gtgcacccgt ggatgtcgca cttcgtacag gtgccggggt cgcgttttca
     4561 cgtcggagag atccgccctc ttcccgagat ggtgggaaag accctgccgg aactcaactt
     4621 cagagtgcgc tataatgcgg tggtattgag cgtcaaccgg tccggcgacc gcttcctccc
     4681 cactgcggaa acagttatcg aggcgggcga tatgcttttg gtcgccggcc ggcgtgaaga
     4741 tctggagaag tgggtcgaag agtgaagcgc cctttcctcc tgttcatcgc cctaccctga
     4801 cgggcagttt tccgcgcacc cggaatgctg cggagcgaac gcctgctctc cggaagaccg
     4861 cttccggcaa gaaggccatt cacgaaacaa ataatggagc gccttcttgt accgatgttg
     4921 atttttaagc ttttttctgt ataatcagag cggcagtaaa tgccaaggcc acacttattt
     4981 ttatttcgag gaggttctcc aatgacaagc acagcacttc gtccagaaga cacgtccgct
     5041 tcagccggcg cactcctctt ctactatccg gcagcctttc tccttttctt cgccgatatt
     5101 ctgatcgtcg atttcccggg aatccgcatc ggcatgctcg acgttcttcc ggcagttccc
     5161 ggtattacgt ggtcgtgcgt cggcgcggtc ttcgccgttc tcatgctcgt tgcgacggtg
     5221 cgctacgtag cgggaggcgg aacgtcggat ctggcgcggc tcttccttcg ggccggacac
     5281 tccgtcgtcg tcctgctttc gttcatcggt ttccttgttg tgccgatggc cgtcacactt
     5341 tccggactct ccgaaagcac tgacacgtac ggcgcgttcg tcgcgttccg cggggccttt
     5401 ttcttcctgt tcctttttgt cctctccggg atgagccatg cgttcctggc aaccggacgg
     5461 accgatagag gcacgacgat cgtcggcgca agtcaaaaac tcctggctgc gcagatggcc
     5521 atgctttcgg cagccttcct tttccttgtt ctgagcgctc ccgggacgct gccctctctc
     5581 tcctgctcca tggatgtgcc ttcatggctt gtctaccttc ttcctgtctg ctttgtcctg
     5641 gtcggggcac ttttcgcatc gttcggggga ggagccgagc acggagcgct tcttggcttc
     5701 atgttccgcc tgtgcgttct tctctccgcg gccgctccgg tagctgtact gctggccgga
     5761 aggtctgaag agtcggggat ggcgctctac atggcgtcgc tcgcgctctt tgtatcggcg
     5821 ctcaaaggac tctatgccgt ggaggcctcc gaagaggctg ccgcatagca aaagctttct
     5881 gctccagtca taagggcgga ccgggttttt tccggttcgc ccttttccct tttctctacg
     5941 ccgttttgag tgtgtctcgc atcaaggact ttctttacca gctgctcgtc cgttgatgtg
     6001 cgggcgcgat atggaggaaa acgcaatgca aaaacaggat acccctttcc ccgtgctttt
     6061 cacgccccgg ctgcgactgt gcgcgatcga atcgtcccat gccgagcggc tgcacgcagt
     6121 ctggaccgat ccgctcgtca tagaatacct cgttctggat ccttttaccg agctgcgtca
     6181 gagcgaggag atgattggcc ttctccagtg gctgcgcccc tccggagagg gcgtccgctg
     6241 ggcgattacg ctgcgctccg atggaacggt gctcggaacg tgcggcttcc acaactggaa
     6301 gaaggaacat gggcgcgcag agatggggta cgaactcgac tcccgtatgt ggcggcaggg
     6361 cttcatgagc gaagccgtct tcgccgcgct ggagtacggt ttcggcacga tggagttgaa
     6421 ccgtgtcgag gcgctggtga cggagggaaa tcagcgatcg agagggttcc tggaaaaggc
     6481 gggcttcagg cttgaaggca cgctgcgcca gtacgaatgg gcgcgcgggc ggtaccagga
     6541 tcagtggatg ttctcgctcc tgaaagaaga tcgtaagggg ggacagttca cttccgtcat
     6601 tgacacaccc gcctctttat gagataaaag aatccggtta atcgtttacg taatcgttta
     6661 ccgcagtgtc cctttgcagg gaaccccatt tttcgggagg cgtgtggtat gaaccggacg
     6721 gcgacaatga aggacgtagc tcgcaaggcg ggtgtttcca ccgcgacggt ttctcacgtg
     6781 ctcaacggaa ccaggcctgt gggaagcgtc ctccgcgacc gcgtacgcca agcggtggcg
     6841 gaactcggct accgtccgaa cttcatcgcc cgcagcctcc gccgcaaagg ctccggcgcc
     6901 gtcggcgtca tcctcatcga cgataccaac ccttttttct ccgaatccac ccgaggcgtc
     6961 aaggacgccg ccctcgaaaa cggatacagc gttctcgtct gtaactcgga gggagacccc
     7021 gaccgggaga acgagtttct tgagctcctt gcggagaagc aagtggaagg ggtcattctc
     7081 ttcaatgttc gcgactccaa cctgcgggag gcggcagggc ggcggctcgc cgtgccagtt
     7141 gtccttgttg atcgcgacgg cgcctctaga ggaatggact cggtcgtcgt ggatcactac
     7201 gccgggggca tggacgcgat ggagcacctt ctttcgctcg gtcacggacg gatcgcctgc
     7261 atcgcaggcc cctcgaactc cacccctgcg ctgctgcgcc ttcgggcata cgcgaacgcc
     7321 ctgcgccgcg ccggaatacc ctacgacaaa tccctcgtct accggggcga cttccgctcc
     7381 gaaagcggct acgccgccat accgttcctc ttctcagatg gtaagctccc cacggcactt
     7441 ttcgctttca acgacctgat ggccttcggc gcgatcaccg ccctcgcgga gatggggctt
     7501 cgcatcccgg aggacgtctc cgtcgtcggt tacgacgaca tactgctctc ttccttcttt
     7561 aatccgccgc tgacgaccgt ggcgcagccg aagaaggaga tggggagggc cgctttcggt
     7621 cttctcctcg acagaatcaa cgacgagtcg ctgcccgcgc gctctctgcg tatgaacgcg
     7681 cggctcgtgg tgcgaaaatc caccgcttcg ccgggtgcgt cgaaaggagg ggatgggtcg
     7741 ggctgtatgc gatgaaccgc tccgggggag ggttgctttt cccgcggggc gcgatgcggc
     7801 acggtgcgct cctggttttc cggacgaatt ccgtccggtc ggtccgtcct tcgaggggcg
     7861 gaaacatact atccacattc agggagggaa acgcatgaaa ggtttgagaa aagttcttct
     7921 gctggttatg gttctggtta ttgcgctctc cgcgttcggc gtcgccggag cggccgagtc
     7981 gaaagtcaag gttgccctgg tcctctccgg cttcctgggc gacaagtcct tcaacgattc
     8041 cgcgtacgag gggctcgagc gctcgatcaa ggaattcggc atcgatctga aggtccttga
     8101 gtccaaggtc ccctccgact gggagtccaa cctcgtggcc atggcggccg aggggtacga
     8161 cctggttctg ggttgcagca cgcagcttca ggagatcatc gccaagcatg ctcccgactt
     8221 ccccgatgtg aagttcggca tcatcgacgg cgtggtgaag gctccgaatg tcatgtccgc
     8281 catcttcgcc cagaacgagg gctccttcct cgccggagct gccgcggcca tgtttacaac
     8341 aaagactgac gttcccggcg tcaacgccga gaagattatc ggctgggtcg gcggcatgga
     8401 cattccggtg cttcacgact tcctcgtggg ctataagcag ggcgctgcct acgtcgatcc
     8461 cgagacccgc gtgctcgtct ccttcgccgg aacgttcaac gaccccctga agggcaagga
     8521 gctgacgctg gcccagttcg agcagggcgc ggacatcgtc atgaacgtcg cgtcgaacac
     8581 cggcaacggc atcctcgagg cggcctacga ggcgaagaag tacgccatcg gcgtcgacct
     8641 gaatcaggac gaagcctacc ccggacacat cctcacctcc atgctcaaga gagtggacat
     8701 gggcagctac ctgatcgtga aagcggtcgt cgagggcaac ttcgagggcg gcaagattct
     8761 cgacatgacc atcgccaacg gcggcgtggg actcaccgac atgtccacga tgaagaaggc
     8821 tctgggcgac aagttcccgc aggacatcct ggagagtatc gcgaagctga ccgaagaagt
     8881 caagggcggc aagatcaagg tcgactccta cgagggattc cagagaaatt agacatcatt
     8941 ttccggaggc ggcggggaga tttccccgtc cgtctccatt gcgaggtggg gttcgtgtcg
     9001 cgtatcctga tcgaaatgaa aggcatctgc aaggaattcc ccggagtgaa ggcggtccag
     9061 gacggactgt tctcgcttga ggagggagag atccactcgc tcataggaga gaacggagcg
     9121 gggaagtcga cgctcatgaa gattctctac ggcatgtacg ttcccgagga ggggagcatc
     9181 gccgtcaagg gagaaacgta tccgtcgctg acgccgaaac aggcgatcgg gctcgggatc
     9241 ggcatggtgc accaggagtt catgctcgtc cgcgagatga cggtgctgga gaacatcatc
     9301 ctcggctttg aaccgaggcg gggagggcgc atcgaccgga gggcggcgct cgaaaaaatc
     9361 gaatactatg cggacaccta cggactccgc gtgcatccgg ataaaaaggt ggaggagatc
     9421 tccgtcgggg aggcccagcg cgtcgagatc atcaagacgc tctacagggg cgcggagatc
     9481 ctcgtcctcg acgagccgac cgccgtgctc acgccgcagg aggccgaaca gctcttctgc
     9541 attctgcgct cgatgaaaga gggcggcaag tccatcgtct tcatctccca caagttgcag
     9601 gaagtgctcg cgatcagcga ccggatcacc gtgatgcggg gaggaaagca catcgcgacg
     9661 gtggcgggat cctctacatc cgctcccgag ctggcgcgcc tgatggtggg gcgcgaggtc
     9721 ttcctcggcg gctcgcccgt cccggcggcg aagggggagg tcgccctctc cgtggagaac
     9781 gtcttcgtcc ccggcgagaa ggagctgtcc aagctgcgcg gcgtctcgtt ctccgtccgg
     9841 aagggagaga tcctcggcat cgccggggtc aacgggaacg gtcagagcga acttgccgag
     9901 gccatcgccg ggcttcgtca ggtgcagcgg gggcgcgtcg tgctcggcgg cgccgaggtg
     9961 cagaacatgc ctccgcggcg tatcagggag catggactgt cgcatattcc ggaggaccgg
    10021 aaccagcgcg gactcgaccg cttttccgac gtgaaggaga acctggccgc ctgttcggtc
    10081 tcggagcctc cgctctcgag ccttggcgtg attcgctggg ggaaggtgcg cgaattcgcc
    10141 gaggggctga tccgttcgta cgacatccgt ccggcgggcg ccgacatcga ggcctccggg
    10201 ctctccggcg ggaacgccca aaaggtcgtc gtagcgcggg aggtctcccg gaaggcagag
    10261 gtgctgctgg cgtcgcagcc cacgcggggc gtcgacatcg gctccatcga gaccatacgg
    10321 aacgagctga accgcgccaa agaagcgggg gcggcgggtg tgctgatctc ttccgatctc
    10381 gaggagatcc tctccctttc cgacaggatc gccgtcatgt tcgagggacg gatcaccgga
    10441 gagctcgatt cctcggaggc cgacgaggag aagctcggcc tgctgatgac tggaggcggg
    10501 acgcatgtat aagacatcct ggggcagcac gctgatgacc atcatcctct cccttgcctt
    10561 gggagcgggg gccatcctgc tgctcggcta cgatcccatc gaagcctact accagctctt
    10621 ccgcggggcc ttcgtgggga aattcaacat cggcggcgtc atggaacgtt tcgtcccgct
    10681 gatgcttacg gcccttgcct tcgcggtctc cgcaaaggtt gccgtgttca acgtcggcgt
    10741 cgagggagag ctctacctgg gcgccatcgc cgcggcgtgg gcggggtacg ctttcaccgg
    10801 gcttcatcct cttgtccata ttcctctctg cttcgccgtc gcaatggtcg cttccggagc
    10861 gtggggggct atccccggcg cgctgaaggc gtactggaag gtgaacgagg tctgcgtcac
    10921 catcctgatg aactacgtcg ccatctactt cacctcctac ctggtaggga accccttctc
    10981 cgcgcgcgcg agcatcccca gaacgcccac catcgaagag tcggcgcgtc tgcttcggat
    11041 tctccgtccc agccgggcga acatcggtat ctttgtcgcg ctcggcgtgc tcgtgctggt
    11101 ctggtggatg ctctatcata ccaactgggg gtacaagctg cgccacgtcg ggcagaaccc
    11161 atctttctcg gagtacgtcg gtattcctcc gaagaaggtg atgatctggg ggatgattct
    11221 ctccggagcc atcggcggtc tcgcgggggc catcgaggtg atgggcatcc acgggcactt
    11281 cctcgacaac ttctcgccgg gaatcgcctt tgacgggatg ctcgcctcgc tgatcgcgaa
    11341 gaacgacatc cgcatggttc ccgtactctc gctcttcctg gccgttctca agacaggggc
    11401 gctggggatg gaacgcttca caggagtccc caagaccctg atcgacacga ttatcgcggt
    11461 cttcatcctg ctcgccgcta tggaggggct cttcgcctat tcgcggcgca gaagcgcccg
    11521 gagctccgga gaggaggctg cgtaatggac ggactgaacg agatactctc cctcgtcttc
    11581 aacgcgaccc tcgtccgctc gaccatccgc gccgctacgc cgattctgta cgccacgctg
    11641 gcgtgcgtcc tgacgcaaca ggcgaacatc ctgaatatag gcatcgaggg catcatgatc
    11701 tttgccgcct tcgcagccgt gaccaccagt tacttcgccg gaagctgggt gctgggcctt
    11761 cttgccgcca tcgttaccgg cgtcgtcgtc gcggccttca tggccttcgc gcatctcaag
    11821 gcgaaggcgg acatcttcgt cgtcggcatg ggggtgaaca tgctcgccat cgcgatgacg
    11881 cgctttctgc tccagcgcac cttcggcgtc tccggcagct ttcaccacaa cgacatcgtc
    11941 ccgatgcctc gcctgaattt cgacttcggc ggcaacgcgc tgctcgatac gctcttcaac
    12001 gactactccc tcttcgagcc ggtcgggatc gtcctcgtct tcgtcctcgc cttcatcctc
    12061 tacaagacgg tgtggggact ccgcctccgc agcgtggggc tcaaccccct ggcagcggag
    12121 acggccggaa tcaacgtgac gttccgcaag ttcgaggtga tgctctactc cggggtgctc
    12181 ggcggcatcg ccggggcgca cctctcgctc gggtactccc gcatgttcgt ggagaacatg
    12241 accaacggcc gcggcttcat gggtgtggcg gcgatgttct tcggcggcgc gaaccccttc
    12301 tgggggtgga tcggctgtct gatcttcggt ttcgcagact ccctcggatc gcggctgcaa
    12361 tcacttggat
//
LOCUS       DAOPKU010000016        12081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_5538_length_12081_cov_5.571262, whole genome shotgun sequence.
ACCESSION   DAOPKU010000016 DAOPKU010000000
VERSION     DAOPKU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12081
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5538_length_12081_cov_5.571262"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="PK849_01045"
     CDS             <1..1364
                     /locus_tag="PK849_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HPE64737.1"
                     /translation="WLARNLASIPLAIRDCMPELVEGLDDWRLMNLPVKDVLDLVDRA
                     RERGRFDEVRDRSVRGTGMAGRDLGVLLVPESKHYSWAKAADIFGIGRENLIPVPVRG
                     DYRMDVRALESIIDSLAAQKRPVLAVVAVVGSTEEGAVDEVHEIVRLRERYAEKGVFF
                     YLHVDAAYGGYARALFLDEENRFMEYDEIKKTHADVGHGGWLVPEVYEAFKAMSSADS
                     ITVDPHKLGYIPYSAGAIVAADRRVIDLISYFAAYVFEKGDDNPMLLGSYILEGSKPG
                     AAVAAVWMAHRVVPLNANGYGRIIESSIEGAFRFHQSILDSTPLEVAGRRFEALPLTR
                     PDLNIVVYAFHEIGNGSLEEMNRLNQAIYEQCSYKSGPLYISDFITSKTSLTEEEYGD
                     APRSFVERFGIEEREWRSCGSVYVLRSCILTPYLVNNTTYEIYWGNFMKSMERAIRRL
                     YES"
     gene            complement(1361..3385)
                     /locus_tag="PK849_01050"
     CDS             complement(1361..3385)
                     /locus_tag="PK849_01050"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPE64738.1"
                     /translation="MITKPVLGVDFGTTKTYLSKCPGDDPSPTSVDFGDGRDGVPSAI
                     LYRKEKEPLIGQSALDEYGEATAWERASFRLVTNFKADLARSEEARAAASDFLSALLA
                     DARKQRLDVEPATRRVLFGIPSEADDAFRAALEATAERAGFGAPFLVDEPKGALLYHM
                     KQRDISVDKALGGVLVADFGGGTCDFAFLEGGRVVRSWGDMLLGGRLFDDLFFQWLCD
                     QNPGLDTELEAQGSAYFVQSHLCREVKEFFSRSMARDRGEPVSKVLAGYGRLTDLTWE
                     EFLRRGASYTPSRPFIEFSKGILPELRGRARSTAWNFLAHNGGEPLQGQKLDLFGWFR
                     ECLRSGLEEGKIERERISLVILAGGSSLWPFVGEILREEIPLREERALVRSDRPYAVI
                     AQGISLLPALKKRFARARQSLQNELPDFLENRLGALTANRVAALEEKMASLAVDIFFR
                     KEVVPLLLEFRKQGGSLALLRKRIDERTAAAEPLAREHLARLAPRFLEGLREEVRDLL
                     AEWFASHGVALPRKDLAFVLPDELSEPMLRVRTPAADGMLDVVGTAVGGLLTAGAAML
                     CGGSSTALITAGPIGLAAGAILGASAGWMLLRYGKARMKETAERMKLPALLAAFMIDE
                     KRIADLENDLSDALADHLERRFVPLKRELDERIRTLVRDEIRALNEINSF"
     gene            complement(3382..4554)
                     /locus_tag="PK849_01055"
     CDS             complement(3382..4554)
                     /locus_tag="PK849_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64739.1"
                     /translation="MIHHFLAPGRKTLALRRSRRYSIGIKRKRGCVLMGRRRKTPVIT
                     TKEEEEIRRRAVEKGVNVYFSYLPPNGVPEQRALESAVRRAEAAASEELLPALLDSVA
                     DEPLPPERRSLLLLRMEERLPALILPEPELLSEIPPLRAAAASAFGTIAGMAFLPSLT
                     WILLGNRAAGVFIGAPLGALFGTLAALLLPRSRKLLLLLAAILGLATVREVIRFLSGG
                     AIFFKGWSLLREKSGSLKGLVLFPLLLATLLLLGARETRFDRKRHEESVRRAFASWTE
                     GAARVALLAASPSAPLQKDEEEAIPLLASRILSLEGACCEDLTAGVSELAAEVRNLGY
                     SDENPAVLFRWNEEAAEKYTSFGAVEPGDAVRVEREPLFRNGELLAKGLVRKVRRS"
     gene            4617..5645
                     /locus_tag="PK849_01060"
     CDS             4617..5645
                     /locus_tag="PK849_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE64740.1"
                     /translation="MRTETLQRGTRFSVILLLCAAALLSAFGQAASASALTVRVLDVG
                     QGDSIFVVFPEGGTMLVDAGTAEAGPRVVETLRSLDAEKIDILVATHPHSDHIGGMLA
                     VLDAFPVGKVWDSGYVHGTRTQQLFLQTVLDRKIRFGRPKAGFSEEFQGVLIEVLAPV
                     KLIYGTDADVNNNSIVLRLSFGEVSFLLAADMETEQRRTVRRFPETTVLKVAHHGSHN
                     GTDARFLAMLRPKTAIISCGKENPYGHPHREAVALLEEAGVDLFTTIGGDVVVETDGI
                     ECTVRKVLESEKSGQEKVRFYIGNRSSRVFHTSACDGLPSERNRVYFKSAEEAVKAGY
                     RPCGRCVQ"
     gene            5642..5857
                     /locus_tag="PK849_01065"
     CDS             5642..5857
                     /locus_tag="PK849_01065"
                     /inference="COORDINATES: protein motif:HMM:NF022659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HPE64741.1"
                     /translation="MRAFIDSVHEGVARVLLGDDESLAVEIPVSWLPEGAAEGKALRV
                     LWELDEEETFCARKAVEDLYDELGDMP"
     gene            complement(5934..7709)
                     /locus_tag="PK849_01070"
     CDS             complement(5934..7709)
                     /locus_tag="PK849_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64742.1"
                     /translation="MKKHVLRIMLAALTLLLVSSACWADMGEIQNFTFRGVGEDVLGP
                     GEDPTPDGKQDYFFSVTVSGIGALSGFILQNEDGNSVWDTVAGNNVPGMTVKDSSGNI
                     IVHSGGSMPLVPLILGAGFTISVHDDGSIARGGKFALLARFADGSRSQSVIEIPASEQ
                     PADAAASVKILSAEWTGTGTRDLTRGDEQLRGDGKADMTARISVQGKGVLTGITIRSV
                     EGEKGAWDTVPANRVPLIAVTQGNKTLNAADGNIEIPLDGKYTFDLLLTDTGAIARGK
                     SRFEVVLVFSDGSVVAKEIAKGKSEEPSSGEFSGSAVFAGPGNRDLVGRNEQRRGNGS
                     PDWKIDLKIETLGTISGIKVFNSTGPAGEWDTIPGNGRWLVAVTGADGKLLNTADGSV
                     RIAVPNPLTLQLWVEDNGSLEDPNVRSKVTLFYDDGRELTREIVSSGSKEDETPTPTP
                     RVRPDRRRERVKREIFFSNPRQASSSDYVGSKDKIGKSGKKDWLFEFRIEGNGAVKQI
                     TVEAVDASFIWDTVPKNRNWVIGVLAPGKGLLNKKDGSVSFEVPPHNNLQLFVEDNGV
                     LKQGASQFEITVTWSDGATSKVSNF"
     gene            complement(7747..8232)
                     /locus_tag="PK849_01075"
     CDS             complement(7747..8232)
                     /locus_tag="PK849_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPE64743.1"
                     /translation="MDETTRGQKTASGIGRTAADSFLSGLYCAESALLAVAESRGTRS
                     ELIPNIATGFCSGMARTCGTCGALTGAIMGIGLALGRSAAAESVEPAYRAVRQLIAEF
                     EEEFGARDCHLLLGCDLGTPEGQAVFREKGLRDRCAEYTGKAAEIAARVIAEIEEERR
                     P"
     gene            8464..8814
                     /locus_tag="PK849_01080"
     CDS             8464..8814
                     /locus_tag="PK849_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPE64744.1"
                     /translation="MFEMHFTVETWGRSVLLSGRLDARSAPDFEKAALAAADEQPEKR
                     LHMLLDFSGLEYISSAGLRSILVLAKRLSPSGGKVVLCGMSGVVEEVFGVSGFDSFLP
                     VTKSREEALRLLGE"
     gene            8829..9257
                     /locus_tag="PK849_01085"
     CDS             8829..9257
                     /locus_tag="PK849_01085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE64745.1"
                     /translation="MENTVRERVFPASLDLLGDIRAFILEEDGAEERLPPKRVAHLDV
                     AIEEIVVNICSYAYETPPGELTIRVEEKPEFFAVDFLDNGVAFDPLAMDEPDVSLPLE
                     KRDAGGLGILLVRRMMDEVHYSRKGTLNSLRIVVDTPRMP"
     gene            9269..10291
                     /locus_tag="PK849_01090"
     CDS             9269..10291
                     /locus_tag="PK849_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE64746.1"
                     /translation="MSMLRALRRLLPAVLLPVFVLLCVCPARSQETAPAASGMTKATL
                     TLQWYPQTQFAGYYMAREKGFYAARGIDLEIRRGGSDVDTIGELREGKTDFATLFLTA
                     GIRYRSQSLPLVNVAQIVNRGNLLVIAYKDRKIRTIKDLNGRKMSLWGDHFSGGYKKA
                     FSDNGIWPVIFPQFISVEVFLRGGVDACSAMSYNEYLRILHTGRRPEDLTVISLRDEG
                     YDFPEDGVYCTEETWRKNPQLARDLREASLEGWRYAAEHPEEAVELILGEADKAGYIV
                     NRLLLRRMLDTILPSIFPEDGSTWTPGVLSRADYERTSEMMKSVFVDMTETAPYDVFF
                     PGGDER"
     gene            10288..>12081
                     /locus_tag="PK849_01095"
     CDS             10288..>12081
                     /locus_tag="PK849_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPE64747.1"
                     /translation="MKERFFPLFPDGRRGVSIAAKLALMIVAGVGAVFLAIVFYGYST
                     SREALEEEFQGRMENLGRAAESQFLQIPMVAEAVTRDLATMITLFEPPPEKMPEMMKE
                     LLASHSQVTGLYVGFSKQATNPLFRNFCPLVFRQGNSIQYKNLAGEMDWTVQDWYQLP
                     LQMGKPLWTEPFFADERAESMMISYGIPLYDANWRYLASIGASLELNWLADIISTMEV
                     GGHGYVFLVTSNGAFVSHPRRELLMRETLFTVAEMENRPDLIPLGHRMIRGEKGVASL
                     YDPLRGDLLVFFRPVGNIGWSLGIVFPEEEVLDDIIHLRRVQTFLGLGGVFAMLLMVF
                     FISNRISGPIRKLKDATMLLASGDLHAPLPPVRGRDEVALLTGSFASMRENLLLHLER
                     LKTETAARERIASELDIARSIQMSLVPRTFPPFPKDGRIDLFAKLEPAREVGGDFYDF
                     FHTDEDHLLLVVGDVSGKGIPAALFMAVTRSFVKALAISSGSCVPPGELMAAVNDRIA
                     EGNDACMFVTLCFVLVDLRDGSFSYACGGHPFPRLLGGDAALPLAPVKGPLVGAMEGM
                     FFEEGGGRLKEGEGIFLFTDGVTEAFDVDESL"
BASE COUNT         2203 a         3748 c         3769 g         2361 t
ORIGIN      
        1 tctggctggc gcgcaacctc gcctccattc ccctcgcgat ccgcgactgc atgcctgagc
       61 ttgtcgaggg cctggacgat tggcgcctca tgaacctgcc cgtgaaggac gtgctcgacc
      121 ttgtggacag ggccagggag cgcggccgct tcgacgaggt gcgcgacagg tccgtccggg
      181 ggacggggat ggccgggcgc gacctcggcg ttctgctggt tcccgagtcg aagcactact
      241 cgtgggccaa ggcggcggat atcttcggca tcggccggga gaacctgatt cccgttccgg
      301 tacgcggcga ctaccggatg gatgtccgcg ccctcgaatc catcatcgac tcactggccg
      361 cgcaaaaacg ccccgtcctc gccgtggtgg cggtcgtcgg cagcaccgag gagggagcgg
      421 tggacgaggt gcacgaaatc gtccggctgc gcgaacggta cgccgaaaaa ggcgttttct
      481 tctacctgca cgtggacgcc gcatacggcg gctacgcacg ggcgctcttt ctcgacgagg
      541 agaatcggtt tatggagtac gacgagataa agaagacgca tgcggacgtg gggcacggag
      601 gctggctggt tccggaagtg tacgaggcgt tcaaggcaat gagcagcgcc gactcgatca
      661 cggtcgatcc gcacaaactg ggctacatcc cgtactccgc gggcgcgatc gtcgccgccg
      721 accggcgggt tatcgacctg atctcctact tcgccgcata cgtcttcgag aagggggacg
      781 acaacccgat gctcctaggg agctatatcc tggaggggtc caagccgggc gcggcggtgg
      841 cggcggtctg gatggcgcac cgggtggtgc ctctgaacgc gaacggttac gggaggatta
      901 tcgagagcag catcgagggc gccttccgct tccatcaatc aattctcgac agcacgccgc
      961 tggaggtcgc cggccgtcgt ttcgaggcgc ttccgctgac ccgtcccgat ctcaacatcg
     1021 tcgtctacgc cttccatgag atcggcaacg gcagcctgga ggagatgaat cgtctgaacc
     1081 aggcgatcta cgagcagtgc tcctacaaat cgggaccgct ctacatcagc gacttcatca
     1141 cgtcgaagac ctcgctgacg gaagaagagt acggggacgc gccgcgctcc ttcgtggagc
     1201 gcttcggcat cgaggagagg gagtggagga gctgcggaag cgtctacgtt ctccgctcgt
     1261 gcatcctcac cccctacctg gtgaacaaca ccacctacga gatttactgg gggaacttta
     1321 tgaagtcgat ggagcgggcg atacggcggc tctacgagag ctaaaagctg ttgatctcgt
     1381 tgagcgccct gatctcgtcc cgcacgagcg tccgaatgcg ttcgtcgagc tcccgtttca
     1441 gcgggacgaa gcggcgctcc aggtgatcgg ccagcgcgtc ggacaggtca ttctccagat
     1501 ccgcgatgcg cttttcgtcg atcatgaagg cagcaaggag agccggcagc ttcatccgtt
     1561 ccgccgtctc cttcatcctc gccttgccgt agcgaagcag catccaccct gcggaggctc
     1621 ccagaattgc gcccgccgcg aggccgatag gtcccgccgt gatgagcgcg gtgctcgatc
     1681 ctccgcagag catcgccgcg cccgccgtca gcagtccgcc gacggccgtt ccgacgacgt
     1741 cgagcatacc gtccgccgcg ggagtccgca cccggagcat aggctccgag agttcgtccg
     1801 ggagaacgaa cgcgaggtct ttccgcggaa gggcgacgcc gtgcgacgcg aaccattccg
     1861 cgagcaggtc gcgtacctcc tcccgaagcc cttcaagaaa gcgcggagcg agccgggcga
     1921 gatgttcgcg ggcgagaggc tcggcggcgg cggtccgctc gtcgatgcgc ttccggagga
     1981 gtgccagcga gccgccctgc ttccggaact ccagcagaag ggggacgacc tccttgcgga
     2041 agaagatgtc gacggcgagc gaggccatct tctcctccag cgctgcaacg cggttcgccg
     2101 tcagcgcgcc caggcggttc tccaggaagt cggggagttc gttctggagc gattggcgcg
     2161 cgcgggcgaa gcgctttttg agcgcgggaa gcagcgaaat tccctgggcg atcacggcgt
     2221 acggccggtc gctgcgcacc agggcgcgct cctcccgcag ggggatttct tcgcgcagga
     2281 tctctccgac gaacggccag aggctgcttc cgccggccag aatgaccagc gaaattcgct
     2341 cgcgttctat cttcccctct tcaaggccgg agcggaggca ctcgcggaac cagccgaaga
     2401 ggtcgagttt ctgcccttgg agcggctccc ctccgttgtg cgcgaggaag ttccacgccg
     2461 tcgaacgggc gcgcccgcgc agctcgggga gaatcccctt ggagaattcg atgaaggggc
     2521 gcgatggggt gtaggaggcg cctctgcgaa gaaactcctc ccaggtcagg tcggtcagcc
     2581 tgccgtaccc ggcgagcact ttcgataccg gctcgccccg gtcgcgcgcc atcgagcgcg
     2641 aaaagaactc cttgacctcc cggcagaggt gggactggac gaagtaggcc gatccctgcg
     2701 cctccagttc ggtgtcgagc cccggattct ggtcgcacag ccactggaag aagagatcgt
     2761 cgaagagccg tccgccgagc agcatatccc cccacgaccg gacgacgcgc cccccctcga
     2821 ggaaggcgaa gtcgcacgtc ccgcccccga agtccgcgac gagcacgccg ccgagcgcct
     2881 tgtccaccga gatgtcgcgc tgcttcatgt ggtagagcag cgctcccttc ggttcgtcga
     2941 cgagaaacgg cgcgccgaaa cccgctcttt cggcggttgc ctccagagcg gcccggaatg
     3001 cgtcgtccgc ttcgctggga atgccgaaga ggacgcgccg cgtcgccggc tccacatcaa
     3061 gccgctgctt ccgcgcgtcg gcgaggagcg cggagaggaa gtcgcttgcc gctgcgcgag
     3121 cctcctcgga acgtgcaaga tccgccttga agttggtgac aaggcggaac gaggcgcgct
     3181 cccatgcggt cgcctcgccg tactcgtcga gcgcgctctg gccgataagc ggttccttct
     3241 ccttccggta gaggatggcg gaaggaacgc cgtcccttcc gtcgccgaaa tccacgctcg
     3301 tcggcgacgg atcgtccccg gggcactttg agaggtacgt cttcgtggtg ccgaagtcca
     3361 cgcccagaac cggcttcgtg atcatgagcg ccgcaccttc ctgacgagcc ctttcgccag
     3421 cagttcgccg ttccggaaga gcggctctcg ctccacgcgc accgcatcgc caggctccac
     3481 cgcgccgaag gatgtgtatt tttccgcggc ctcttcattc catcggaaga gcacggccgg
     3541 attctcatcg gagtagccca ggttgcgcac ctccgcggca agctcggaga cgcccgcggt
     3601 caggtcttcg cagcaggcgc cttcaaggga gaggattctc gacgcgagga gcggaatggc
     3661 ctcctcttcg tccttctgca gaggagcgga cggcgacgcc gcgagcaggg cgacgcgcgc
     3721 cgccccctcg gtccatgatg caaaggcacg gcgaacggac tcctcgtgac gtttccggtc
     3781 gaaacgggtc tcgcgcgctc ccaggagcaa gagcgttgcg agaagcagcg ggaaaagcac
     3841 gagccccttg agcgacccgc tcttttcgcg cagaagcgac caccccttga agaagatcgc
     3901 gccgccgctg aggaagcgga tgacctctct tacggtcgcg agccccagaa tggcggccag
     3961 cagcaggagc agcttccggc tccgggggag caggagcgcc gcaagggttc cgaagagcgc
     4021 gccgagcgga gccccgatga agacccccgc ggcccggttg ccaaggagaa tccaggtaag
     4081 cgaaggcaaa aacgccatac cggcgatcgt tccgaaagcg gatgccgccg ccgcccgaag
     4141 tggaggaatc tcggagagca actcgggctc cggaaggatg agcgcgggaa ggcgctcttc
     4201 catgcgcagc agaagcaacg aacgccgctc cggagggagc ggttcgtcgg cgacggagtc
     4261 aagaagcgca gggagcagct cctccgacgc cgcagcctcg gctctcctga cggcgctttc
     4321 aagggctctc tgctccggaa cgccgttcgg cggcaagtac gagaagtaga cgttgactcc
     4381 cttttcgacg gcgcgacgac ggatctcctc ttcttctttt gttgtaatca caggcgtttt
     4441 tcttctcctg cccatcagaa cgcaccctct ttttctcttg attccaatag agtagcgcct
     4501 cgaccgtcga agagcaagcg tttttcggcc gggggcgagg aaatgatgta tcatggtatg
     4561 cacgcctcta cgcggcggca tccgcgaaag gcgcgacaga gaggagtgta ctgagaatga
     4621 gaacagaaac gcttcagaga ggaacgaggt tcagcgttat ccttctctta tgcgcggcgg
     4681 cgcttctttc ggccttcgga caggcggcct ccgcgtcggc tttgacggtg cgcgttctcg
     4741 acgtcgggca gggggattcg attttcgtcg tttttccgga gggcggcacg atgctcgtcg
     4801 acgccggaac cgccgaggcc ggaccgaggg tcgtcgagac gctccgttcg ctcgacgccg
     4861 aaaagatcga catccttgtg gcgacgcacc cgcactcgga ccacatcggc ggtatgctcg
     4921 ccgttctgga cgccttccct gtcggcaagg tatgggacag cgggtatgtc cacgggacac
     4981 ggacgcagca gcttttccta cagacggtgc tcgaccggaa aattcgtttc gggcggccga
     5041 aggcgggctt ttcggaggag tttcagggcg tgctcattga agtgctggcg cccgtgaagc
     5101 tgatctatgg gaccgacgcc gacgtaaaca acaactcaat cgtcctccgt ctctccttcg
     5161 gagaggtctc cttcctgctg gccgccgaca tggagacgga gcagcggcgt acagtccgcc
     5221 gctttcccga gacgacggta cttaaggtcg cgcaccacgg ctcgcacaac ggaacagacg
     5281 cgcggtttct cgcaatgctc cgcccgaaga cggccatcat ctcctgcggc aaggagaatc
     5341 cgtacggaca cccgcaccgg gaagccgtgg cgctgttgga agaggccggc gtcgacctgt
     5401 tcacgacgat cgggggcgac gtcgtcgtcg agacggatgg aatcgagtgt accgttcgga
     5461 aagtccttga atcggagaag tccggtcagg agaaagtgcg tttctatata ggaaaccgtt
     5521 ccagccgggt gttccataca tccgcgtgcg acggtctccc ttccgagaga aaccgtgtgt
     5581 acttcaaaag cgcggaagaa gctgtaaaag ccggataccg gccgtgcggg aggtgcgttc
     5641 agtgagggcg ttcatcgatt cggtgcatga gggggtagcg cgcgttctgc tcggcgacga
     5701 cgagtcgctc gccgtggaga tacctgtctc ctggctgccc gagggggcgg ccgaggggaa
     5761 ggcgctccgg gtgctgtggg agcttgacga agaagagacg ttctgcgcgc ggaaggccgt
     5821 ggaggacctc tacgacgagc ttggcgacat gccgtgagga aatgcgctga acggatggga
     5881 gagctacaga aaaagggaga aggcgctcgt ccttctccct tcgtcaccgc agattaaaaa
     5941 ttggatactt ttgaagtggc gccgtcgctc caggtcaccg tgatttcaaa ctgcgacgct
     6001 ccctgtttca acaccccgtt gtcctccacg aaaagctgaa ggttgttgtg cgggggaacc
     6061 tcgaaggaga ccgatccgtc cttcttgttc aacagcccct tccccggagc cagtacgccg
     6121 ataacccagt tcctgttttt cgggacggtg tcccagatga acgaagcgtc cacggcctct
     6181 accgtgatct gcttgacggc gccgtttccc tcgattctga attcgaagag ccagtctttc
     6241 ttgccgctct tacctatctt gtccttcgat ccgacgtagt cgctgcttga ggcttggcgg
     6301 ggattcgaga aaaagatctc ccgcttcact ctctcacgcc gtctgtcggg gcgcactctc
     6361 ggagtgggcg tcggcgtctc atcctccttc gagccggagg agacaatttc tctggtgagc
     6421 tctcttccgt cgtcatagaa cagtgtgact tttgagcgga cgttcggatc ttcgagggaa
     6481 ccgttgtcct ctacccagag ttggagcgtc agggggttcg gaacggctat ccggacggag
     6541 ccgtccgcgg tgttcaggag ttttccgtcc gctcctgtga cggcgacgag ccagcggccg
     6601 tttccgggga tcgtgtccca ctcgcccgca ggtccggtcg agttgaaaac ttttatcccg
     6661 ctgatcgtcc cgagcgtctc gatcttgagg tcgatcttcc agtcggggct tccgttgccc
     6721 ctgcgctgct cgtttctccc tacaagatct ctgtttcccg gaccggcaaa aacggcggaa
     6781 cccgagaact cgccggaaga tggctcctcg ctctttccct ttgcaatctc tttggcgacg
     6841 acagatccgt ccgagaagac gaggacgact tcaaagcgcg acttcccgcg ggcgatcgct
     6901 cccgtgtccg tcagcagcag gtcgaaagtg tatttgccgt cgagaggaat ttcgatgttt
     6961 ccgtccgctg cgttcagcgt cttgttcccc tgcgttactg cgataagagg aacgcggttg
     7021 gcaggcacgg tatcccaggc gcccttctcc ccttcgacgc tccggatggt gatgccggtg
     7081 agcacgccct tgccctggac ggaaattcgg gccgtcatgt cagcctttcc gtcgccgcgg
     7141 agctgttcgt ctccgcgggt aagatcgcgc gtccccgttc ccgtccattc agcggagagt
     7201 atcttgaccg aagccgccgc gtccgccggt tgctcggaag cgggaatctc gatgacggat
     7261 tggctccgcg aaccgtcggc gaatcgcgcc agcagggcga actttccgcc gcgcgcaata
     7321 gatccgtcgt cgtgcacgga gatcgtgaat cccgccccga ggatcaacgg aaccaacggc
     7381 atgctgccgc cgctgtggac gatgatattg ccgctgctgt ccttcaccgt cattccgggg
     7441 acgttgttcc cggcgacagt atcccacacg gagttcccgt cctcgttttg cagtatgaac
     7501 ccggaaaggg cgcctatccc tgaaacggtc accgaaaaga aataatcctg tttcccgtcc
     7561 ggcgtagggt cttcgccggg gccgagcaca tcctcgccga cgcccctgaa cgtgaagttc
     7621 tgaatttctc ccatgtcagc ccagcaggcg gacgaaacca gcaaaagtgt caatgccgcg
     7681 agcatgatgc gcagaacatg ttttttcacc tgaaccatct cctttgtagg tatttccccg
     7741 ttattgttat gggcggcgct cttcctcgat ttccgctatg acgcgcgccg caatctccgc
     7801 ggctttcccc gtgtactcgg cgcagcgatc gcgaagcccc ttctcccgaa aaacagcctg
     7861 cccctccggc gtcccaaggt cgcacccaag cagcaggtga cagtcgcgcg cgccgaactc
     7921 ctcttcgaac tcggcaatga gctgcctgac cgccctgtag gcaggctcca ccgactcggc
     7981 cgccgcagac cggcccaacg cgagtccaat ccccataatt gcgcccgtta acgctccgca
     8041 cgtcccgcag gtacgcgcca taccgctgca aaagcccgtc gcgatgttcg ggatcagctc
     8101 ggagcgtgtc ccgcgggatt ccgccactgc aagcagcgcg ctctcggcgc agtagaggcc
     8161 cgagagaaat gagtccgcgg ctgtccttcc gattccggaa gctgttttct gtcctcgtgt
     8221 tgtttcgtcc atcttttcct ccttgaaaaa tgacatgatg acgggtcgat attggtcgac
     8281 aataacataa attccgtcgt ctcgcaagaa gcggaatcgg caagagaata ttcgactgca
     8341 tcccgtttct ccgcttttct tgacacgtcc ggtaaaaacg cgcacaatga acttcaagaa
     8401 cgcctgtatt cgtttcggca gtagtgtggt ggttctgtat gctgctttca gggggaatga
     8461 actatgttcg aaatgcactt tacggtcgaa acctgggggc ggagtgttct gctctcggga
     8521 cgtctggatg ccaggtcggc tccggatttt gagaaggccg cgctcgccgc agccgatgaa
     8581 cagcccgaaa agcgtcttca catgctcctc gatttttcag gactcgagta catcagcagc
     8641 gcgggactcc gttcgattct cgtgcttgcc aagcggctct ctccttcggg agggaaggtg
     8701 gtgctttgcg gaatgtccgg ggtcgtcgag gaggtcttcg gcgtttccgg cttcgacagc
     8761 tttcttccgg tgacgaagag ccgcgaggag gctcttcgcc ttctcggcga gtagaggagg
     8821 aaaagacgat ggaaaacacc gtcagagagc gggttttccc agcctctctc gacttgttgg
     8881 gcgatatccg tgccttcatt ctggaggagg acggggcgga ggagcgtctt ccgccgaagc
     8941 gggtggcgca cctggatgtt gcgatcgagg agatcgtcgt gaatatctgt tcctacgcgt
     9001 acgaaacccc tccgggcgag ctgacgatac gggtcgagga gaaacccgag tttttcgccg
     9061 tggattttct ggataacggg gtcgcgttcg accctctcgc catggacgag cctgacgttt
     9121 ctcttcccct tgaaaagcgc gacgcggggg ggctgggcat cctcctggta cggagaatga
     9181 tggacgaagt gcactactcc cgcaaaggga cgttgaacag cctccgtatc gtggtggaca
     9241 cgccccggat gccgtagggg gattggacat gtcgatgttg cgcgcccttc ggcggctgct
     9301 tccggccgta cttctcccgg tgtttgttct tctctgcgtt tgccccgccc gaagtcagga
     9361 gacggccccg gccgcttccg gcatgacgaa ggcgacgctc acgcttcagt ggtacccgca
     9421 gacccagttc gccgggtact acatggcgcg tgagaagggc ttctacgcag cgcgcggtat
     9481 cgacctcgaa atccggcgcg gcggctcgga cgtcgacacc atcggagagc ttcgcgaagg
     9541 aaagacggac tttgcgacgc tcttcctgac ggccgggatc cgctaccgct cccagagcct
     9601 gccgctggtg aacgtcgccc agatcgtcaa ccggggcaac ctgctcgtga tcgcctacaa
     9661 agaccgcaaa atccgcacga tcaaggatct gaacggacgg aagatgagcc tgtggggcga
     9721 ccatttctcc ggcggatata aaaaggcttt cagcgacaac ggtatctggc cggtgatctt
     9781 tcctcagttc atctccgtgg aggtcttcct gcgcggaggg gtggacgcct gttccgcgat
     9841 gagctacaac gaatatctgc gcattctgca cacaggacgc cgcccggagg atctgacggt
     9901 gatctcgctc cgggacgaag ggtacgactt ccccgaggac ggcgtctact gtacggagga
     9961 gacgtggaga aaaaatccgc aactggcccg cgatctccgc gaggcctccc tcgaagggtg
    10021 gcgctacgcc gccgagcacc ctgaggaggc cgttgagctg attctcggag aggccgataa
    10081 agccgggtat atcgtgaacc ggcttctgct gcggcggatg ctcgacacga tactcccctc
    10141 gatcttcccg gaggacggct ccacatggac gcccggcgtt ctgtctcgcg ccgactacga
    10201 gcgcacctcc gaaatgatga aaagcgtctt cgtcgatatg acggagacag ccccgtacga
    10261 cgtctttttc cccgggggtg acgagcgatg aaggagcgtt ttttccctct ctttcccgac
    10321 ggacgcagag gcgtctccat agcggcgaag ctggcgctga tgattgtcgc gggcgtcggc
    10381 gccgttttcc tcgccatcgt cttctacggc tactccacat cgagggaagc gcttgaggag
    10441 gagttccagg ggcggatgga gaacctcggc agggccgcag agagtcagtt cctccagatt
    10501 cccatggtgg cggaggccgt gactcgcgat cttgctacca tgatcaccct cttcgagccg
    10561 cctccggaga agatgccgga gatgatgaag gagcttcttg cgtcccattc gcaggtcacc
    10621 gggctgtatg tcggcttttc aaagcaggcg acgaatccgc tctttcgcaa tttctgtccg
    10681 ttggtttttc gccaggggaa ttccattcag tataagaacc ttgccggcga aatggactgg
    10741 acggtgcagg attggtacca gcttccgcta cagatgggca agcctttgtg gacggagccg
    10801 ttcttcgccg acgaacgcgc cgaatcgatg atgatcagtt acggtattcc cctctacgac
    10861 gcgaactggc gttatttggc ctctatcggc gcgtcgctcg aactcaactg gctggccgac
    10921 atcatctcca cgatggaggt cggaggtcac ggctacgtct ttcttgtgac gtccaacggc
    10981 gcgtttgtct cccatccgcg gcgggagctg ctgatgcgcg aaacgctctt caccgtggcc
    11041 gagatggaga accgccccga cctcatccct ctcggccaca gaatgatacg gggcgagaag
    11101 ggggtagcgt ccctgtacga tcccctccgc ggcgacctcc tggtcttttt ccgtccggtg
    11161 ggaaatatcg gatggtcgct cggcatcgtc ttccccgaag aagaggttct cgacgacatc
    11221 atccatctgc gccgggtgca gactttcctc ggtctgggag gagtattcgc catgctgctg
    11281 atggtttttt tcatctcgaa cagaatcagc gggccgatcc gaaaactgaa agacgccacc
    11341 atgctcctcg cctccggcga tctccacgcg ccgctccctc ccgtgagggg ccgcgacgag
    11401 gtcgcgctgc tcaccggctc gttcgcctcc atgcgggaga atcttctgct tcaccttgaa
    11461 cgtctgaaga ccgagaccgc ggcgcgcgag cgcatcgcca gcgagctgga catcgcccgg
    11521 tccattcaga tgagccttgt tccgcgcacc ttccccccgt tcccgaagga cggccgcatc
    11581 gatctcttcg ccaagctcga accggcgcgc gaagtcggcg gcgattttta cgacttcttc
    11641 cacacggatg aagatcatct gctgctggtc gtcggcgacg tctccggcaa ggggatcccc
    11701 gccgcgctct tcatggccgt gacgcggagc ttcgtcaagg cgcttgccat ctcctccgga
    11761 agctgcgttc ctcccggaga gctgatggcc gccgtcaacg accgcatcgc cgaggggaac
    11821 gacgcctgca tgttcgtcac gctctgcttc gtcctcgtgg atcttcggga cggttcgttc
    11881 agctacgcct gcggagggca tcccttcccc agactgctgg gcggcgacgc tgcgctgccg
    11941 ttagctccgg tgaaagggcc gctcgtcggg gcgatggagg ggatgttctt tgaagaggga
    12001 ggcggacgac tgaaggaagg ggagggcatc ttcctcttca ccgacggcgt gacggaggcg
    12061 ttcgatgtcg acgagtcgct g
//
LOCUS       DAOPKU010000017        12023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_5567_length_12023_cov_4.804228, whole genome shotgun sequence.
ACCESSION   DAOPKU010000017 DAOPKU010000000
VERSION     DAOPKU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12023
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5567_length_12023_cov_4.804228"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..514
                     /locus_tag="PK849_01100"
     CDS             185..514
                     /locus_tag="PK849_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPE64748.1"
                     /translation="MKRPQRKDGNTTLSLRDLPNGSLARIVKILSPLHNAAETGSSCC
                     AARLEALGLRVGKIVEKVSGMPFHGPVTLLLDGRQIAVGWNISSHVLVVPVKERERAH
                     GRSAERE"
     gene            489..2264
                     /locus_tag="PK849_01105"
     CDS             489..2264
                     /locus_tag="PK849_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HPE64749.1"
                     /translation="MDEVRNESKKLLLVGNPNVGKSALFTRITGVHAVSSNYPGTTVG
                     FLEGWLRRSGDVWRVIDVPGAYTLSPTNEAEEVAERMVNEGADVAVLVLDATALERNL
                     FLAFQTMERRLPVIIALNMVDEARHKGIDVDPEALERLLGVPVVPTVAVTGQGISRLL
                     DRLPEARPARIALPGGYASETGVPGREERWSAIGEIIEQVQRVSHRHHTLLDKLEDAS
                     VDSFWGLIIGVLVIVASFASIRFLGEGIIDEFFDPLFEAWLLPVAERLSLFLGGEGLF
                     HDIVVGRLIDGHIDFEQSFGLLTTGLYVPLAMVLPYVAAFYFVLSLLEDFGYLPRLAV
                     IFDALLHRMGLHGFAIVPSLLGLGCNVPGILATRVLDSDRERFIAATLISVAIPCAGL
                     QAMILGTVGQLGMRWVFAVYATLILSWLILGRLLHSILPGFSPELIVEIPPYRIPSFK
                     SLGLKLWIRIRGFLLEAVPLVLGGILVVGLLDSTGVTAFLSRLLAPVFNGLLGLPAEA
                     SGPILLGILRKDVAMGMLGALGLSPVQLVVATVTLSMTFPCIATFIVLWKELGGRRMA
                     ASMGIMVFSALVAGTAVRLLLSIHF"
     gene            complement(2334..2777)
                     /gene="aroQ"
                     /locus_tag="PK849_01110"
     CDS             complement(2334..2777)
                     /gene="aroQ"
                     /locus_tag="PK849_01110"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPE64750.1"
                     /translation="MKRILMLHGVNHNMFGKRDPAQYGTITLDEIDEKLRTLGAELGV
                     EVVGFQTNSEGAMCERIHQAFQEGMDGVIINAGAWTHYSYAIRDALAILTVPVAEIHM
                     SNIHAREEFRHRSVFAEVVKGQICGFGLDSYLLGLRAVVSAIGGA"
     gene            complement(2858..3655)
                     /locus_tag="PK849_01115"
     CDS             complement(2858..3655)
                     /locus_tag="PK849_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPE64751.1"
                     /translation="MQGHTTDEKTTVTGTGEEKSVRSVERAFSILMCFTLDEDELTLM
                     DIARKMHLPTTTVSRMVNTLVGLGFLEKSPGKTYSLGANLYYLGAVARNHFKLQKIVY
                     PYMKQVRDTTKEAVSLYVLDGDARVCYEHVESLLSMRCVVRVGDRFPLWAGASGKCLL
                     AYADPELVDRELAKAKPITPTTIISREAFLADLETIRKKGHAISYGEREEGITSIAVP
                     VFHPKNKVTTVFSVAGPSVRFTDEIIEELLPVVKAMTEEISRSLSYR"
     gene            complement(3700..4593)
                     /gene="aroE"
                     /locus_tag="PK849_01120"
     CDS             complement(3700..4593)
                     /gene="aroE"
                     /locus_tag="PK849_01120"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPE64752.1"
                     /translation="MFNAKVNTRFFGLLGNPVGQSAAPFMHNSVYQKLGLDMLYVPLE
                     LEMESLETVVRTMQLFNYAGTGVTMPFKTQVHKYVDGLADSARYTEVVNTIVIGKDGR
                     KIGHNTDGTGFVESLEKQAGLNIPDHRYLLLGSGGAATAIACALAMRGAKSIRSLCIR
                     QDYSYAETLSSRVNAHFPGVCEIGEMNDTTIRKGLEEFDVIIHATKVGFYPKTGDILF
                     DPALLSSSHLVCDVVYVPMETKLLVEAEKRGCRTLSGLWMNVYQAAEQMRLWAGIEEP
                     PIDFMYQCGIEYLRREGKLTA"
     gene            complement(4616..5443)
                     /locus_tag="PK849_01125"
     CDS             complement(4616..5443)
                     /locus_tag="PK849_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPE64753.1"
                     /translation="MQPQFSLAQLTVLKTSPAEISRIAADCGYDYVSMRQIYMGLPEE
                     PDYDLSKNKKLMAETKAVFADTGIKLLDIELARIFDGMDVKKYEPAMTTAVELGGKHV
                     LSSIWTLDREYAIERFAELCDLAAQYGLTVELEYVPIAGVRTLGGAVDVLRKVNRKNA
                     GLMIDIHHFHRAQDDPAELAKLPKEWFRFAHLCDAPAEIPSEQTEMVRILREARSYVG
                     EGGIDIAPILNAMPVVPYSIELPNLERVAESGYAEHARRCLESAKAFCAGHLKGRSE"
     gene            complement(5641..7062)
                     /locus_tag="PK849_01130"
     CDS             complement(5641..7062)
                     /locus_tag="PK849_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPE64754.1"
                     /translation="MAKKELTEAQRQTLETVFARARAAEKIIENYDQERVDRMCRCIA
                     WAAANPRTFDRFCKMGVEESGAGDWSGRYGKRHKILGVLRDALRQKSVGIVEVNPEKG
                     LVRYGKPAGLITSLIPMTNPELTPIVTAIYALKARDVVVFSPHPRTRKTTFEVVESMR
                     QGLKSIGEPEDFLQCITEVNMTIVEELMKMGDLVMATGGLAMSRAAHSSGKPAYCSGA
                     GNATMIFDETADVEEAARNTRISKTSDFGSGCSADGNLVIHGSIYEKMVAQLVKEGGY
                     LANDEEREKLKKAMWDDEGHRIVETVAVAPQKLCKAAGFEIPEDRKFVMVVGEGIGKE
                     FPFSGEKLTTLLALYRYEGEFENALKMIDEIYKVGGRGHSCGIYSFNEEHIHALALRA
                     PVTRIMVRQPQSKANAGSANNGMPMTSSMGCGTWGGNQVSENIALKHYMNSTWVAKPI
                     LTDAPSEEVLFGEFYDPSNTREG"
     gene            complement(7141..8940)
                     /locus_tag="PK849_01135"
     CDS             complement(7141..8940)
                     /locus_tag="PK849_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPE64755.1"
                     /translation="MRKDLVAFQLVKFLEARGVEKVFGLCGHTVIGFLDALKESKITF
                     ITVRHEQIAAHAADGYARGKGCRVPGVLLTHLGPGLANATTGVAEAGLNSIPMVVIAG
                     DVPSCYFGRHPHQEVNLHADASQYEIYRPFVKRAWRVDRPELLPEVMDKAFRLAVTGR
                     PGPVLVSVPMDIFSMEIDTRFFEQRMHNMPLLPRPGLDVKTAEDIAALLAEAKSPVLY
                     PGGGVISSGAASALTELATFLEIPVLYTLMGKGSIPDDHPLAVGMTGFWGTEFNNSAA
                     MKADVMMAVGTRLSEADCSSWYFGETFDVPPTKLIHIDINQEEIGRNYTTEIGAVCDA
                     KMALDAILAAAKRKYPNGVKRPELIGEIAEAKAAYRATIAEAQKSDQFPMRPERILAD
                     LREALPRDGFVVADVGWNKNGVGQQFDIYEPGTFVAPGGLATMGYGPSAALGVKVACP
                     DRKVVALIGDGGMGANVSPFATAAVENIGVVWVVMNNCAFGTIAGLERQHYDHEFGTL
                     FEREGKPYSPDFAAIARAYGIDGYTVERAEDFKPMLEKALASDKPCVLDVRMENAPVV
                     TKGCWNINDIFRKRGEEKPWRVWSWEEIEPWRM"
     gene            9268..10206
                     /locus_tag="PK849_01140"
     CDS             9268..10206
                     /locus_tag="PK849_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPE64756.1"
                     /translation="MTMTLPKSIFFCEVGPRDGLQNEPATLSVERKVRLIEGVVDAGV
                     RIVEIGSYVNPKAVPQMADTDEVARAIRRMEGVEYRALVANLKGVERALASGVNKVKL
                     TVSASESHAKANLNKTPSELIDGFKSCTEFARSNGAEVSGAISTAFGCPFEGKVPPEK
                     VAEIAERLHDLGITELSLSDTTGMANPRQVYDLATSMQEMLPDVRWFLHFHNTRGMAL
                     ANTLAGLQAGIVRYDASLAGLGGCPFAPGASGNVASEDMIHMLHEMGVDTGIDLDKLL
                     DLARELRGWVGHDTDSALLKAGPCCRPIPRDGAAKQ"
     gene            complement(10337..11509)
                     /locus_tag="PK849_01145"
     CDS             complement(10337..11509)
                     /locus_tag="PK849_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPE64757.1"
                     /translation="MKEVVVGVVGGGFASHFHSRCFARVSGFSVRLKAIADVEIEKAK
                     AVAEAYRYEEYHDDYSALLRDPEIDVVDICTPPFLHPRITMEAMEAGKHVICEKPLAG
                     YFGSPGDIEPIGDTVPKSRMYQSVLTEIETMKAALEGSDRLFMYAENYVYSPNILKAA
                     EIVRAKKSKILFMKGEESLKGSTSPVAGRWDKTGGGSLIRVGCHPLGGILWLKREEAR
                     AGGAQIGVHSVLCDTGRMTPGLDEYERRHISVRPVDVEDYASVALTFSDGTKALVIAG
                     DTMLGGTRNYVEVYANDAVMHCRITPSDVMSTYMLDEEGLENVYISEMLPAKTGWQQP
                     FVAEEHLRGYAAQFQDFIECAATGRAPLSDFELACETIRVIYAAYLSAEEGRRILL"
     gene            complement(11846..>12023)
                     /locus_tag="PK849_01150"
     CDS             complement(11846..>12023)
                     /locus_tag="PK849_01150"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE64758.1"
                     /translation="VTPPLGVRLFVGCGISGITLESISKAVWPFILSMIVAVLLLTYI
                     PWISIILPRLAGIY"
BASE COUNT         2415 a         3756 c         3229 g         2623 t
ORIGIN      
        1 aatgatacgg cgacgacgga gcgcatcaag gagttcgcag ccattcttgc caaactcggc
       61 atcgaaaacg agatccgcaa ggaaaaagga acggacatcg acgctgcctg cggccaactg
      121 cgggcgcaga gcatgtaagg gggtcgtcag aagagtgaac ctcctccaac gtgtcgcccg
      181 ctccttgaaa cgcccgcaaa ggaaggacgg caacacgacg ctttcgttgc gcgacctccc
      241 gaacggatct ttggcccgga ttgtcaagat cctgtctccg ctgcacaacg cagcggagac
      301 aggatcttca tgctgtgccg cacggcttga agctcttggg cttcgcgtcg gcaagatcgt
      361 tgaaaaagtg tccgggatgc cctttcacgg accggtaaca ctgcttctcg acgggcgaca
      421 gatagccgtc ggatggaata tttcgtcaca cgtactggtt gttcccgtga aagaaagaga
      481 gcgcgctcat ggacgaagtg cggaacgaga gtaaaaagct tctgctcgtc ggcaacccga
      541 acgtcggcaa gagcgccctc ttcacccgaa tcacaggcgt tcacgccgtt tcgtcaaact
      601 accccggcac gaccgttgga ttcctggaag gctggcttcg ccggagcggc gatgtgtggc
      661 gtgtaatcga cgttcccgga gcgtacacgc tctcgcctac gaacgaagcc gaggaagtag
      721 cggaacgaat ggtcaacgag ggagcggatg ttgccgtgct tgttctcgac gcgacggccc
      781 tggaacgcaa cctctttctc gccttccaga cgatggagcg gcgcctgccg gttatcatcg
      841 cgctcaatat ggtcgacgaa gcccgccaca agggaattga cgtcgatccc gaggcgttgg
      901 aaagactgct cggcgttcca gtcgtcccta cggtcgcagt gaccggacag ggaatcagcc
      961 gtctgctgga ccgtctcccg gaagcccgtc ccgccaggat cgcgctcccc ggagggtacg
     1021 cttccgaaac cggcgttccc ggcagggaag agcgctggag cgccatcggt gagataatcg
     1081 aacaggtcca gagagtcagt cacaggcacc acacgctcct cgacaagctt gaggacgctt
     1141 ccgtggactc tttttgggga ctgattatag gagttctcgt catcgtcgcg agctttgcct
     1201 ccattcgctt ccttggagag ggaatcatcg acgaattttt cgacccgctc tttgaagcgt
     1261 ggctgctccc tgtagccgag cgtctttcct tgtttctggg aggggaaggg cttttccatg
     1321 acatcgtcgt cggccgcctc atcgacgggc acatcgactt cgagcagagc ttcggcctgc
     1381 tcaccaccgg tttatacgtc cccctggcga tggtcctgcc gtatgtcgca gcgttttact
     1441 tcgtcctcag cctcctcgag gacttcggtt acctcccgcg cctcgccgtc atcttcgacg
     1501 cccttcttca ccgaatgggc ctccacggct tcgccatcgt cccctcgttg ctcgggcttg
     1561 gctgcaacgt cccgggtatc ctcgccacca gggtgctgga ctccgaccgg gagcggttca
     1621 tcgccgcgac ccttatctcg gtcgcaatac cctgcgccgg acttcaggcg atgatactgg
     1681 gaacggtcgg acagctcggc atgcggtggg tttttgccgt ctatgcaacg ctcatccttt
     1741 cgtggctcat acttgggcgc ctgttgcact ccattctccc cggattcagt ccggaactta
     1801 tcgtggagat cccaccctac cgcatcccgt cgttcaaatc gcttggactg aagctctgga
     1861 tacgaatccg cggatttctc ctcgaagctg tgccgcttgt ccttggaggc attctcgtcg
     1921 ttggtctcct cgactcgaca ggcgttaccg catttctttc gagacttctt gcccctgtct
     1981 tcaacggcct gctcggtctc ccggccgagg cgtccggtcc cattctcctc ggtatcctgc
     2041 gaaaggacgt ggcaatgggg atgctcggcg cgctcggact ctcgccggta cagcttgtcg
     2101 tcgccacagt gacgctgtcg atgaccttcc cgtgcatcgc caccttcatc gttctctgga
     2161 aggagctcgg cgggcgtaga atggcggcct ccatggggat catggttttc tccgctcttg
     2221 tcgccggaac agctgttcgc ctgttgctct cgattcactt ctgaagcgag tcaaataaga
     2281 gggagtggga ggggcgaagt cgcccctccc actccctctc gaagtgttgt ttgctacgcg
     2341 ccgcctatcg cgctgacgac cgcgcgcagg cccaacagat agctgtcgag gccgaaaccg
     2401 cagatctgcc cctttacgac ctccgcaaag accgaacgat gacggaattc ctccctggcg
     2461 tggatgttcg acatgtgtat ctccgccacg ggaacagtca ggatcgccaa ggcgtcccga
     2521 atcgcgtagc tgtagtgcgt ccatgccccc gcattgataa tgacgccgtc catgccctcc
     2581 tgaaaggcct gatgaatccg ttcgcacatc gcgccttcgg aattcgtctg aaagccgaca
     2641 acctcgacgc caagctcggc gcccagcgtg cgcagcttct cgtcgatctc gtcgagcgtt
     2701 atcgttccgt actgcgccgg atcgcgcttt ccgaacatat tatgattcac accgtgaagc
     2761 atgagtattc tcttcatggc cctgtttcca tctccctttc gttgttcgag tcgactgaac
     2821 gttttctatg atggagccca acgaagcaaa gaactcgtta cctgtaggaa aggctccttg
     2881 aaatctcctc cgtcatcgcc ttcaccacag gaagcagctc ttcgataatt tcgtcggtaa
     2941 agcgaaccga cggaccggcg acggagaaca cggttgtcac tttgtttttc gggtggaaca
     3001 caggaacggc gatggaggta attccctcct cgcgttcccc gtagctgatt gcgtgtcctt
     3061 ttttgcggat cgtctcaagg tcggccagga acgcctccct cgatattatc gtggtcggcg
     3121 ttatcggctt cgccttcgcg agttcgcggt ccacaagttc agggtcggcg tacgccaaga
     3181 ggcacttgcc cgaagctccc gcccacagag ggaaacggtc tccgacacgg accacgcaac
     3241 gcatgctgag aagactctcc acatgttcgt agcacacccg agcatccccg tcgagaacgt
     3301 agagcgacac cgcctccttt gtcgtatcgc gaacctgctt catatatggg tagactatct
     3361 tctgcagttt aaaatggttc cgtgcgacag cccccagata gtacagattc gctccgagcg
     3421 agtaggtctt cccgggactt ttttcgagga agccgagacc tactagggtg ttcaccattc
     3481 gagagactgt cgtcgtcgga aggtgcatct tccgtgcgat atccatcagc gtcagctcgt
     3541 cctcgtcgag agtgaagcac atcaggatgg aaaacgcccg ttccactgaa cggacagact
     3601 tttcctcccc ggttccggtc acggtcgttt tttcgtctgt tgtatgccct tgcatgacat
     3661 ccccctctag aacgccccgc tcccactctc gtgggaagct caggctgtca gtttaccttc
     3721 tcttcgaaga tattcaatac cgcactggta catgaagtca ataggaggct cttcaatacc
     3781 cgcccagaga cgcatctgct ccgccgcctg gtagacgttc atccacaggc cggagagagt
     3841 gcggcatcct cgcttctccg cctctacgag aagttttgtc tccatgggga catagaccac
     3901 gtcgcacacg agatgtgacg aactcagaag cgccggatcg aagaggatgt cgccggtctt
     3961 cgggtagaac ccgactttgg tcgcgtggat gatgacatcg aactcctcca gccccttgcg
     4021 aatcgttgtg tcgttcatct cgccgatctc gcagacgccg ggaaaatgag cgttcactct
     4081 tgaagagaga gtctccgcgt aggagtagtc ttgccggatg cagagcgaac gtatcgactt
     4141 cgcccctctc atcgcgagag cgcaggcgat cgcggtcgcg gcgcctcccg agccaaggag
     4201 aagataccgg tgatccggga tgttcagccc ggcctgtttc tcaagcgact cgacaaaacc
     4261 cgttccgtcg gtattatgac cgatctttcg tccgtctttc ccgatgacga tggtgttgac
     4321 cacctcggtg taccttgcgg agtcggccag cccgtcgacg tacttgtgga cctgtgtctt
     4381 gaagggcatc gtaacccccg tgcccgcata attgaagagc tgcatggtgc gaaccactgt
     4441 ttcaagactc tccatctcca gctcaagtgg aacgtagagc atgtcgagtc ccagtttctg
     4501 gtagacgctg ttatgcataa agggcgcggc gctctgtccc accgggttgc ccagaagacc
     4561 gaagaatcgc gtgttcacct tcgcgttgaa catattctcg aactccttcc ccggcctact
     4621 ctgaacggcc cttgagatgt ccggcgcaga aggctttcgc cgactccaga cagcggcgtg
     4681 catgctcagc gtaccccgac tccgccacgc gctccaggtt cggcagctcg atcgagtagg
     4741 ggacgacggg catcgcgttc agaatcgggg cgatgtcgat ccccccctcc cccacatacg
     4801 atctggcctc cctgagaatc cgtaccattt ccgtctgttc cgaggggatt tccgcgggcg
     4861 cgtcgcagag atgcgcaaag cggaaccact ccttcggcag cttcgcaagc tccgcagggt
     4921 cgtcctgggc tctgtggaag tgatgaatat cgatcatcag cccggcgttc ttccggttga
     4981 ccttccggag cacatcgact gcgccgccca gcgtcctgac cccggcgatg ggaacatact
     5041 ccagctcgac ggtcaggccg tactgcgcgg cgagatcgca caactccgcg aagcgctcta
     5101 tggcatactc cctgtcgagc gtccagatgc tgctcaggac atgcttgccc cccagctcga
     5161 cagccgtcgt cattgcgggt tcgtactttt tgacgtccat gccgtcgaat atcctcgcga
     5221 gttcgatgtc aagcagcttg atacccgtat ctgcgaagac ggccttcgtc tccgccatca
     5281 gttttttatt cttcgagaga tcgtaatcgg gctcctcggg gagccccata tatatctgcc
     5341 gcatgctcac atagtcgtat ccgcaatccg ctgcaatccg cgagatctcg gcaggcgacg
     5401 tcttcagcac cgtaagctgc gccagggaaa attgaggctg cataatccgt actctcctcc
     5461 tccgaatgta aaaaaacgtt ctcgaacgac ggcattcgtc attaggccgt gtcgggggaa
     5521 tcctttccga cgaaaaccac gccgacgttt tccatcatca gaaagactgt gtcgagcggt
     5581 acattcacga atccgaaaaa acggatcttc tttccgaagc gttcgcgctg tggaaagaag
     5641 ctacccctcc cgtgtgttcg aaggatcgta gaactcgccg aagagcacct cttcagaggg
     5701 agcatccgtc aaaatcggct tcgcaaccca tgtgctgttc atgtaatgtt tcagtgcaat
     5761 gttctccgaa acctggtttc cgccccaggt gccgcacccc atgctcgacg tcatgggcat
     5821 tccgttgttt gcgcttcccg cgttcgcctt cgactgcggc tgccggacca tgatacgggt
     5881 gacgggcgcc ctgagagcca gcgcgtggat gtgctcctcg ttgaagctgt aaataccgca
     5941 ggagtgcccg cgcccgccga ccttgtagat ctcgtcgatc atcttcagcg cattctcgaa
     6001 ctccccctcg tatctgtaga gcgcaagcag cgtcgtgagc ttctccccgg agaaggggaa
     6061 ctccttgccg atcccctccc ctacgaccat gacgaacttc ctgtcctcgg gtatctcgaa
     6121 cccggcggcc ttgcagagct tctgaggagc gaccgcaacc gtctctacaa tccggtgtcc
     6181 ttcgtcgtcc cacatcgcct tcttcagttt ctcccgctcc tcgtcgttgg cgagatagcc
     6241 accctccttg acgagctgcg ccaccatctt ctcgtagatg gagccgtgga tgaccaggtt
     6301 gccgtccgca gaacagccgg aaccgaagtc ggacgtcttg ctgatgcgcg tgttccgtgc
     6361 cgcttcttca acgtcggctg tttcatcgaa gatcatcgtc gcattcccgg cccccgagca
     6421 gtaggcgggc tttccggagc tatgggcggc cctgctcatc gcgagccctc ccgtcgccat
     6481 gacaaggtcg cccatcttca tgagctcctc cacaatagtc atattgacct cggtgatgca
     6541 ctggagaaaa tcctcgggtt cgccgatcga cttcagacct tggcgcatgc tctcgacaac
     6601 ctcgaacgtc gtctttctgg ttctggggtg cggagagaag acgaccacat cccgagcttt
     6661 cagcgcgtaa atcgccgtga cgatgggcgt caattcagga ttcgtcatcg gaatcaggga
     6721 tgtgatcagg cccgcgggct ttccgtaacg gaccagtcct ttctccgggt tgacttcgac
     6781 gatcccgacg ctcttctggc gcaatgcgtc gcgcagcacc ccgaggatct tgtgcctctt
     6841 tccgtagcgg ccgctccaat ctcccgcgcc gctctcctcc acacccatct tgcagaatcg
     6901 gtcgaaggtg cgaggattgg ccgccgccca ggcaatgcag cgacacatcc tgtccacacg
     6961 ctcctggtcg tagttttcta tgatcttctc cgccgcgcgc gctctcgcga agaccgtctc
     7021 cagagtctgt cgctgcgctt cggtcagttc ttttttcgcc atagcatcgt catctccttc
     7081 ttccattcgc cgcgttcagt ccggcgtaca gctcgaaatt tgccgtggtc gcttgccggt
     7141 ctacatcctc cacggctcga tctcctccca ggaccatacc ctccacggtt tctcctcgcc
     7201 gcgctttcta aaaatatcgt tgatgttcca gcaccccttc gtcaccacgg gagcgttttc
     7261 catccggacg tcgaggacgc agggtttatc ggacgcgagt gccttttcca gcatcggctt
     7321 gaaatcctcc gcccgctcga ccgtgtatcc gtcgatgccg tacgccctcg caatggcggc
     7381 gaaatcaggg ctgtacggct tgccttcgcg ctcgaagagc gtgccgaact catgatcgta
     7441 gtgctgtctc tcaaggccgg cgatcgtacc gaaggcgcag ttgttcatca caacccagac
     7501 aacgccgata ttctccaccg ccgcggttgc aaacggcgag acgttcgctc ccataccccc
     7561 gtcgccgatg agcgcgacga cctttctgtc agggcaggcg acctttacgc cgagcgccgc
     7621 cgagggaccg taccccatag tcgccaaacc gccgggtgcg acaaaggttc ccggttcgta
     7681 aatatcgaac tgctgcccga cgccgttctt attccagccg acgtccgcca ccacgaagcc
     7741 gtctcgggga agcgcttccc gaagatccgc caggatgcgc tccggccgca tcgggaactg
     7801 atccgacttc tgcgcctctg cgatcgtcgc acggtacgcg gccttggctt cggcgatttc
     7861 tccgattaac tccgggcgtt tcacgccgtt cggatacttc cgcttcgcag cggcaaggat
     7921 agcgtcgagg gccatcttcg cgtcgcacac cgcgccgatc tccgtagtgt agttacggcc
     7981 gatctcctcc tggttgatgt cgatatgaat aagcttcgtc gggggcacgt cgaaggtctc
     8041 gccgaagtac cacgagctgc aatccgcctc cgaaagcctc gtaccgaccg ccatcatcac
     8101 gtccgccttc atcgcggcgc tattgttgaa ctccgtcccc cagaaaccgg tcatgcctac
     8161 ggccagcgga tgatcgtccg gaatggagcc tttgcccatg agcgtataga gcaccggtat
     8221 ctcaagaaat gttgcgagct ccgtcaacgc ggacgccgca ccagaagaga taacgccgcc
     8281 ccccgggtag agcaccggac tcttcgcctc ggcgaggagc gcggcgatat cctccgctgt
     8341 cttcacgtcg agccccggcc tggggagaag cggcatattg tgcatccgct gctcgaagaa
     8401 gcgggtatcg atctccatcg agaagatatc catcggcacg gagacgagaa ccggtccggg
     8461 acgtcctgtg acggcgagac ggaacgcctt gtccatcacc tcgggaagca gctccggtct
     8521 gtccaccctc caggcgcgct tcacaaacgg cctgtagatc tcgtactggc tggcatccgc
     8581 atggaggttg acctcctgat gaggatgacg gccgaaatag caactgggga cgtcgcctgc
     8641 gatgacgacc atagggatag agttgagccc cgcttccgcg acgccggtcg tcgcgttggc
     8701 cagccccggc cccaggtgcg tcagcagaac gcccggaacc cggcacccct tcccgcgcgc
     8761 atagccgtcg gccgcatgcg cggcgatctg ttcatggcgt accgtaatga acgtaatttt
     8821 gctctctttc agagcgtcca gaaaaccgat gacagtgtgc ccgcagagac cgaagacctt
     8881 ctccaccccc cgggcctcca gaaacttcac gagctgaaac gctaccaggt ctttacgcat
     8941 attcttccgc acctccgttg aaaagttcgc aatcaaccgt ttttatgatg ctggattccc
     9001 cgtacgattg agagtagaaa atcccgtaga acggaaacaa aaaccatctc aaagaaatga
     9061 gtacacaata atctatcgcc gtcgcctttg tcaatacttc ctcttctttt tttctgtcac
     9121 ccggaaaaca ccttcgttgt atcggcgctt ctccatcact tcgaattccc attgaacgga
     9181 aatataaacc gtttgacagg aattcgtttt ttgagtactg ttgtcccgga gagaggttta
     9241 tcatcagtat gcaaaggagg gatagcaatg accatgacac tgcccaaaag cattttcttt
     9301 tgtgaagtcg gcccccgcga cggtcttcag aacgagccgg ccacgctctc cgtcgagcgg
     9361 aaagtccgct tgatcgaggg cgtcgtcgac gccggggtca ggatcgtgga aataggctcg
     9421 tacgtcaatc caaaggcggt tccgcagatg gccgataccg acgaggtagc acgcgccatc
     9481 cgccggatgg aaggtgtgga gtaccgagcg cttgtcgcca atctcaaggg agtggaacgc
     9541 gccctcgctt cgggagtgaa caaggtgaag ctcacggttt cggccagcga atcgcatgcg
     9601 aaggcgaacc tcaacaaaac accgtccgaa ctgatcgacg ggttcaaaag ctgcacggag
     9661 tttgcccgtt cgaacggagc cgaagtttcg ggagccatct ccaccgcctt cggctgcccc
     9721 tttgaaggga aggtacctcc cgaaaaggtt gcggaaatag ctgaacgtct gcacgacctc
     9781 ggcatcaccg aactctccct ttcggatacc accggcatgg cgaacccgcg gcaggtttac
     9841 gatctcgcaa cctctatgca ggagatgctc cccgacgtcc gatggttcct tcacttccac
     9901 aatacgcgcg gaatggccct tgcaaatacg cttgccggtc tccaggccgg gatcgtacgt
     9961 tacgacgcgt cgcttgcggg cctcggcggt tgccccttcg cccctggcgc ttccggaaac
    10021 gtcgcttccg aggacatgat tcacatgctt catgagatgg gcgtcgacac ggggatcgac
    10081 ctcgacaaac tactcgacct tgcgagggaa ctccgcgggt gggttggaca cgacacggac
    10141 agcgcgctgc tgaaagcggg tccgtgctgc cgccccatcc ctcgggacgg cgccgcgaag
    10201 cagtgagagc agccgcacgc cgcagaaaga ccgcatatcc ctaccaatcc agtgaaggcg
    10261 agacgcagac gaaaaggcga ctcgctttca ctgtgaaatg ctctgtcagg agtttacggt
    10321 cgcctccgaa tggcgtctac agcagaatgc gacgcccctc ctcggcagag agatacgccg
    10381 cataaatgac gcggatcgtt tcgcaggcca gttcaaaatc cgaaagtgga gcccttcccg
    10441 tagctgcgca ctcgatgaag tcctggaact gcgccgcata cccccgaagg tgctcctcgg
    10501 caacaaaagg ttgctgccag cccgtctttg cggggagcat ctcggagata tagacgtttt
    10561 ccaacccctc ttcgtcaagc atataagtgc tcataacatc ggacggcgta atccggcagt
    10621 gcatgacggc gtcgttggcg tacacctcta cgtaattgcg cgttcccccc agcatggtat
    10681 cgccggcgat gacgagcgct ttcgtcccat cgctgaaagt aagggctacc gaagcgtaat
    10741 cctcgacgtc gacaggacgc acgctgatat gacgccgttc atattcatcg aggccgggcg
    10801 tcattcgtcc ggtatcgcac agaacgctgt gcacgcctat ttgcgctcct ccggccctcg
    10861 cctcctcccg cttgagccag agaataccgc cgagagggtg gcacccgacg cggatgagcg
    10921 aaccgccccc ggttttatcc catcgtccgg cgactggaga ggttgatccc ttcaggcttt
    10981 cctcaccctt catgaagaga attttgcttt tcttcgcccg cactatctcg gcggccttca
    11041 gaatgttcgg cgagtagacg tagttttcgg cgtacataaa gagcctgtcg cttccttcaa
    11101 gcgccgcctt cattgtttct atttccgtca acacggactg gtacattctg gacttgggaa
    11161 cagtatcgcc tatcggttcg atgtctcccg gagagccgaa gtacccggcg agcggttttt
    11221 cacaaatgac atgcttcccc gcctccatcg cctccatcgt gattcgcggg tgcagaaaag
    11281 ggggagtgca tatatcgacg acgtcgatct ccggatcgcg caaaagcgcg ctgtaatcgt
    11341 catggtactc ctcgtaacga taggcttccg caacggcctt cgccttttcg atctcgacgt
    11401 ccgcgatggc tttcaaacgc acggaaaacc ccgaaaccct tgcgaaacat ctgctgtgaa
    11461 aatgcgaggc gaatccgcct ccgacaacgc ccacaacaac ttctttcatt ctgtgtcacc
    11521 tcgcgattgt gaagaaaaag taagaaggaa aaaagagctc tccgagcgag ggcagaggat
    11581 tctccgctca aaaaagcgca ccatatcccg ctctacggga ataacatccg ctaaaaggac
    11641 ttcgaggtta caatacaaaa cacggcatac cctgtcaaga ctatttccat attcaaagat
    11701 cagcataaca agagcgattt gttgatttat cgacacgctt gcgctgactt ggcgtatccg
    11761 ttcgagtatc gcgatctctt tagtccaatt gaaaaagcgc cccgaaacga tacgtttcga
    11821 ggcgcttttg cgtgcagcaa gttttctaat aaatccccgc cagacgagga agaatgatgc
    11881 ttatccaagg gatatacgtc agcagtagca ccgcgacaat catgctcagg atgaacggcc
    11941 acaccgcttt ggagattgat tccagcgtga tcccgctgat tccgcaaccg acgaagaggc
    12001 ggacgccgag cggaggcgtg acc
//
LOCUS       DAOPKU010000018        11816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_5697_length_11816_cov_5.034776, whole genome shotgun sequence.
ACCESSION   DAOPKU010000018 DAOPKU010000000
VERSION     DAOPKU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5697_length_11816_cov_5.034776"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PK849_01155"
     CDS             <1..1300
                     /locus_tag="PK849_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HPE64759.1"
                     /translation="EALRLRKADDEEGTSAHPVGAEEAWERFEPFLKRTGVTRVSDIT
                     QLDRIGIPVFNVLRPSVNGYTAAHGKGFTRTAARLSAAGESLERWYATCTSTPSFRGT
                     WNELSATYTMIPPERMAMTPYSFFHKDLEIPWTLGWDIVHQEEIPLPLELVRLSPGGP
                     DSALMSLDEGFLFQCSSNGLSCGVHFLEAVSQALLEVVERDSVTASTMGARGKGIPGA
                     VFRAALPETIPFPKVQELFKKIRSAGVEVLLCDNGTDVGVPTWNCYLFDMEDSPGEIG
                     GVAHGMGSSLDHETAMIRAVTEAVQGRCVFLSGVRDIVPSKEFAGFMNVGARTALKAM
                     KSSVGEWLDMSGVKDVPFATFEEDVHETVRRLSAVGLDRVIVYRLSRPDDPVQAVKVV
                     VPGAEGYILPYYQPGERGVTAIRRGFEEGSPLFRTEGFSW"
     gene            1294..2697
                     /locus_tag="PK849_01160"
     CDS             1294..2697
                     /locus_tag="PK849_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HPE64760.1"
                     /translation="MVAVIFGGPSLEGVDRTQSSALFLPPAVQGDLLRAVREHRPSVI
                     GLLDCDLPYRSLPAWHKEILFALSRGIAVVGAAAVGAHRAAELAPNGMEGVGGIYERV
                     ASGELERDDEVLCDWEETPDGIRRLTLPLVTMRAALSAAKDAGVLSGRSAASLLSHAE
                     GLFWRKRTWKAFFAAADDISQDELHSFESWLPNAPDPVKEDAEALLRRVEELASKSGE
                     SGSPLPNDSAGKGDPGEASALFKSLDQRDRPVPTSNGTIRQWMIGDMVSFAHPEAEDI
                     NGRGLNRRLALLLAALWGIVPRTEEIEAEKQRLRRRFKLKSDEAFAAWKKENDLDEEE
                     MSRLLKEEALLHKLHRWYMQGRIHGRNTGAVLDELKLRGGYAEWKERAGRRDALLRSR
                     PEVLREERERSCSTPFMTLVREHMRAEGFPWHGAMLPEAIPETGMEARDLLDELLLAR
                     AARRILGNATNCGAGEA"
     gene            2753..3064
                     /locus_tag="PK849_01165"
     CDS             2753..3064
                     /locus_tag="PK849_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HPE64761.1"
                     /translation="MSMESAVAFYERVESDVELQEKLTELGTKENVSQYVRDELGYDF
                     TLEEMQKVIFERHPEMTDEELEAVVGGTTASEIGKAAATGTAIGLGLCALASLLAAAA
                     A"
     gene            3348..4646
                     /locus_tag="PK849_01170"
     CDS             3348..4646
                     /locus_tag="PK849_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin system secretion protein"
                     /protein_id="HPE64762.1"
                     /translation="MKKQIFRQSALDRLSSPEQLDMLFQVVRPAYWAVLSALLLLLCV
                     LVVWGFYGTVTTMVSGQGILLREGARVDASSPGSGRLEAIFVDIDDSAGKGQVIARLA
                     QPDLRHQIAEMRGRVEILRAERAMGTEMGADKKMLESEYLARRRQALNSSIKVARDRA
                     KSLREQLEQYDALYAKKYITRTQYLEVKDRYNAVLQEILSFNEDLARLPMTAADTTSQ
                     IERQRIDVDMRLLTAEKQLAALEERYDLETSIRSPEAGRVLEIFKLEGQVISAGEALL
                     SLEKNDDTGAPLYVHAYVQPQDGKKILPEMEAQISPSVVKQEEFGVLRGRVTQVSSFP
                     ASARGMTRVLGNEQLVQALSRGGASIAVTMELYPSDKTESGYVWSSRNGPPLALQSGT
                     LCGVTVVVRRQAPATLVLPFLKKFFLGVGEQERDGGAKDA"
     gene            4639..6894
                     /locus_tag="PK849_01175"
     CDS             4639..6894
                     /locus_tag="PK849_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="HPE64763.1"
                     /translation="MPDRPAAKQTPPPGGASLAPSGQFPSWKNRRVVTPTMLQMEVTE
                     CGAASLGIVLGYYGLHLPLETLREECGISRDGSKATNLLKAARRHGMEAKGYRKEPQT
                     LGDLPLPIILFWNFNHFLVLEGIKGNRVYLNDPAAGPRTVDRETLDRSFTGVAISIVP
                     GENFHPEGRPRSPWAALGRRLAGARTALLFAVLAGLALVIPGLMEPAFQQIFVDRLLV
                     EGRLEWFRPMLTLMAIVALVKGALTWLQGSVLLRQELAMAIRDSASFMNHLLKLPYTF
                     FLQRYAGEIGNRVQLNDRVAQLLSRELATTVLQCILVLFYGVMITLYDPPVSVIALGA
                     AAGCALLIISVNRRRTDLNERFTGEVGKFAGAAIGGLSVIETIKSFGGEEDQFARLAG
                     YLSKIADCRIELSRLAVRIQAILPMLTALAAAGVLCYGGFRVMDGVLTMGMLLALQTL
                     MTQTINPVTELVTLWDSFQTAQGNLNRLDDVLRYPRMPERPALEDGAMAAPKHPGQAR
                     ARSVRLSGRLELRDVSFGYSKLGEPLIRDFSLTLVPGSRVALVGGSGSGKSTVARIVC
                     GLFEPWSGEVLLDGRLLATISREETATSFAFVDQEISLYEGTIRENLSLWDPSVPTEQ
                     LVAACRDAAIHKDVMARQGGYDGIVEENGRNFSGGQRQRLEIARALVRSPSLIVLDEA
                     TSALDAPTEEAIDRALRRRGCTCLIVAHRLSTIRDCDEIIVLHQGKVAERGTHEELLG
                     LGGRYAGLIEN"
     gene            6922..9861
                     /locus_tag="PK849_01180"
     CDS             6922..9861
                     /locus_tag="PK849_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin export ABC transporter
                     permease/ATPase subunit"
                     /protein_id="HPE64764.1"
                     /translation="MVGTLPFEALRPLGTEQEITGKTPFFLDDPETVWCVLSGQIDVY
                     VTAREGALQTGGRDHLFTMEAGEALFGVEPGLFGASMGLLAGGIPGTRVLRISRSLLL
                     EQTDGEGTAIAAFLADRFLEGLGRGIVKDIVPLPRIKQSIPAGRTELGPNRPAGSETL
                     LWTEMEEGRALFVGTEDLAPGSGLFALPPNCWIVPLEQCVVRSIPTGELLRRGEFAAV
                     FDAFCEMIFSSLAMNARLRAADDLGALRQMQMSSRRALSSALGTLARALSGKRPREFA
                     SREESPIRRAFATVAVFLGRSAVFPRGGEIAAAQGEGRESLRDLARASNLQLRRVTLA
                     GPWWTSDGAPLIAFMNAPGDGLSGRGDGRSGTPVALLPSGRGYRLVDDGEERPVDEQI
                     AASIAPDAYQVYRPLPGSPLGGRDLLRFGLAGAKRDMGAVLLISVFLALLGLISPEVN
                     RQIFGEVIPQAERGRMLQLFAILVSVAVSSGLLHLANGTAFLRTESVMDHDVSAGIWD
                     RILRLPVGFFRKTTSGDLANRALGLFMLRETASRTVKTLLVQSVFLVFFLGQSFWYDW
                     RLALAGLGCLLIPAVLMVAVNLFQLRFQRKMIGLQNRLLALTFQIMTGISKIRVAGAE
                     ERSFARWAELFGAQRMLGTKARRLEVFLNTVMALFPLSVAIVILFALVRWSAADASYE
                     TGRFMAFWSAFGALQGAFFQMVSSFTATLNMLPLVENLAPILRAEPETGDMKTDPGEI
                     TGRIDLEGVVFRYLKDGPPVLKGLDLHIEAGEFVAVVGPSGAGKSTLIRLLLGFDQPE
                     SGGIFYDNKDIAELDLGKLRRQIGVVLQSGGLLSGDIASNVRCSRNLTAGQIEDALRT
                     AGMEEDIAAMPMGIHTVITDGANTVSGGQKQRLLIARAVAGKPRVLIFDEATSALDNR
                     TQAMISRSLENIQATRIVVAHRLSTVIGADRICVLEDGRLVEEGKYDELMAKDGLFAE
                     LVRRQTV"
     gene            complement(9953..11665)
                     /locus_tag="PK849_01185"
     CDS             complement(9953..11665)
                     /locus_tag="PK849_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE64765.1"
                     /translation="MKIFLCNVSKKSDTNAHDYVGLFYLAGALEEAGFEPLVFHGKPE
                     HLLDEIEKNKPRAVGFSCDFDNVRIIEDMSGKIKERWGIPVVVGGPQSIALGRDFLEK
                     SGADYILRGEGEVSLPRLLDAVLKDEGDVDGVKGLARIQDGVFLENPPEIIEDLDALP
                     LPAYSASLHADHAYGNLIFTGRGCPYRCAYCAPSPGKRKVRLRSIPLVLEEIKVNLKN
                     NPGLKCLIVMDDTFTLGRERIEEFCRGMTELRKERDLVWYCECHVGRIRDFMDILPAM
                     IDAGLIRLQIGIESGDQNVIDQYNKHVKIDDIVEFVTYAADCGLAQIATNFIVGGPAE
                     KDGATASLIRTLIDRAPGVIDIITGFLRAYPGTEIFENPGKFNLQLHDESGRRTGDDY
                     PFVTPVGCSQDQVMRQRQELNRVIRRSMQQKIKHRGLKEDAVRKQFEAAFKYGIRSRW
                     FAEISANPRAYEYYYVLFLNEGQAFEESLDFSRDYPQRTFELWRAMTFSDGFPQLDGM
                     ALSPLEYELLLRCAGKRSLKKIQTELYEVFGGSYENEREFHRQLIDMLHRFDSKYWLT
                     HFVV"
BASE COUNT         2160 a         3605 c         3761 g         2290 t
ORIGIN      
        1 cgaagcgctc cggttgcgga aggccgacga cgaggaagga acctctgccc atcccgtggg
       61 ggcggaagaa gcgtgggagc gctttgagcc gtttttgaag cgcacgggtg tcacacgggt
      121 ttccgacatc acgcagctcg accggatcgg tattccggta ttcaacgtac tgcgccccag
      181 cgtcaacggg tacaccgcgg cccacggcaa ggggttcacc aggacagcgg cgcgcctctc
      241 ggccgccggc gagtcgctgg aacgctggta cgcgacgtgc acgtccaccc cctcctttcg
      301 gggaacgtgg aacgaactgt ccgcgacgta caccatgatc ccgccggagc ggatggcgat
      361 gacgccatat tcgttctttc acaaggatct ggagattccc tggacgctcg ggtgggacat
      421 cgtgcatcag gaggagattc cgctgccgct cgagctggtc cgtctctccc ccggaggccc
      481 cgacagcgcc ctgatgagcc tcgacgaagg gtttctcttc cagtgcagct ccaacggact
      541 ctcctgcggc gtccactttc tggaggccgt ttcacaggcg ctgctggagg tcgtcgagcg
      601 cgactccgtg accgcatcca ccatgggggc ccgcggcaag ggaatccccg gcgcggtctt
      661 tcgcgccgcc ctgccggaga cgattccgtt tccgaaggtg caggagctgt tcaaaaaaat
      721 acgctcggcc ggtgtcgagg tacttctctg cgacaacggg actgacgtcg gcgtgccgac
      781 ctggaactgc tacctcttcg atatggagga ctccccgggg gagataggcg gggtggccca
      841 cggaatggga agctctctcg accatgaaac cgcgatgatc cgtgctgtaa ccgaggctgt
      901 tcaggggagg tgcgtcttcc tctccggtgt gcgcgacatc gtcccgtcca aggagttcgc
      961 cggattcatg aacgtcggtg cgcgcaccgc gttaaaggca atgaagagct ccgtcggcga
     1021 atggctggac atgagcggtg tcaaggacgt tcccttcgcc acgttcgagg aggacgttca
     1081 cgagacggtc cggcggctct cggccgtggg gctcgaccgg gtgatcgtct accgcctttc
     1141 ccgtcccgac gatccggtcc aggccgtgaa agtggtggtt cccggagcgg aggggtatat
     1201 cctcccgtac tatcagccgg gagaacgggg cgtcacggca atccgccgcg gattcgaaga
     1261 aggaagccca ctcttcagaa cggagggatt ctcatggtag cggtcatctt cggcggtccc
     1321 tccctggagg gcgtcgacag aacacaatcg agcgcgctct tcctgccgcc cgccgtacag
     1381 ggcgatcttc tacgggcagt tcgcgaacat cgcccgtcgg tgatagggct gctcgactgc
     1441 gatctgcctt accggagcct gccggcatgg cacaaggaga tcctcttcgc tctctcccgc
     1501 gggatcgcgg tcgtgggtgc cgctgcggtc ggggcgcaca gggccgccga gctggcgccg
     1561 aacggtatgg agggagtcgg cggaatctac gaacgtgtgg cttcgggcga actggaacgc
     1621 gacgacgaag tgctctgcga ctgggaagaa acaccggacg gcatccggcg cctcacactg
     1681 ccgctggtga cgatgcgcgc cgctctgtcg gcggcgaagg atgccggagt cctctcaggg
     1741 aggagtgccg cttcgctgct ctcccacgcg gaagggctct tctggagaaa gcgcacctgg
     1801 aaagcgttct tcgccgccgc ggacgacatt tcacaggacg agctgcactc gttcgaatcg
     1861 tggctgccga acgcccccga ccccgtgaag gaggacgccg aagcgctgct gcgccgcgtg
     1921 gaggaactcg cctccaagag cggggaatcc ggtagtcctc tcccgaacga ttccgctgga
     1981 aaaggagacc ccggcgaggc atcagcgctc ttcaagtcgc tggaccagcg cgaccgcccg
     2041 gtgcccacgt cgaacggcac gatccgccag tggatgatcg gcgacatggt cagcttcgcc
     2101 caccccgagg cggaggacat caacggcagg gggctgaacc gtcgtctcgc gctgctgctc
     2161 gccgcactct gggggatcgt tccccgcacg gaagagattg aagcggagaa acagcgtctc
     2221 cgccgccgct tcaaattgaa gagcgacgaa gcgttcgccg catggaagaa ggagaacgac
     2281 ctcgacgaag aggagatgtc ccgtcttctg aaagaggaag cgctgctcca caagctgcac
     2341 cggtggtaca tgcaggggcg aatacacggc aggaacacgg gcgcggtgct cgacgaactg
     2401 aagctccggg gcggatatgc cgagtggaag gagcgtgccg gaagacgcga cgcattgctg
     2461 cgcagccggc ccgaggttct ccgggaggag cgggagcgct cgtgttcgac tcccttcatg
     2521 acgctggtgc gtgaacacat gagggcggag ggctttccgt ggcacggggc gatgctgccc
     2581 gaggcgatac cggagaccgg gatggaagcc cgcgaccttc tggatgagct gctgctggcc
     2641 cgcgccgccc ggaggattct ggggaacgcg acgaactgcg gcgccggaga agcatagaga
     2701 gttccgacgc ccgttccgca tggaagggcg aattcacagg gaggtgttgc agatgtcaat
     2761 ggaatcggcg gttgcgtttt acgagcgggt tgagagcgat gtcgagttgc aggagaagct
     2821 gacggagctt ggcacgaagg agaatgtctc acagtacgtg agggatgaac tggggtacga
     2881 cttcaccctg gaagagatgc agaaggtgat cttcgagcgc catccggaga tgaccgacga
     2941 ggaactggaa gcggttgtcg gcggaacaac ggcatcagaa ataggcaagg ctgcggcaac
     3001 gggtaccgct ataggtttgg gactctgtgc gctggcatca ttattggcag cagcagcggc
     3061 atagcggaat ttaccgggat tcccggtttc ggaggtgtcc tctgtgttaa ccgagcgtct
     3121 tttcgaaatc gaatatccgg gaccgttttt tgaggagggg aaattcttcc ctcctttttg
     3181 tgtaggcggc agctccgaaa aaccggacag agcaccggca gaaaaaaagc gcagccgtct
     3241 ctgtttcttc cggaagcatc ggggtataat tcaatggaca acaggatttc tcgtgcgcat
     3301 accggaaaaa gccggtatgg ctttttcttg aaggagcgtg cccgaccgtg aagaaacaga
     3361 ttttccgcca gtcggctctc gacaggcttt cctctcccga acagctcgac atgctctttc
     3421 aggtcgtccg gcccgcgtac tgggcggtgc tttccgcgct gctgctttta ctctgtgtgc
     3481 tggtcgtctg ggggttctac ggcacggtga ccacgatggt ctccgggcaa ggcattttgt
     3541 tacgggaagg agcgcgtgtg gacgcctcct ctccggggag cgggcgcctg gaggccatct
     3601 tcgtcgatat cgacgactcc gctgggaagg ggcaggtgat cgcgcggctc gcccagcccg
     3661 acctgcggca tcagatcgcc gaaatgcgcg gcagggtgga gattctgcgt gcagagcgtg
     3721 cgatgggtac ggagatgggt gccgacaaaa agatgctgga atcggaatac ctcgcccggc
     3781 gcagacaggc tctgaactcc tccatcaagg tcgcccggga tcgtgccaag tcgctgcgtg
     3841 agcagctgga gcagtacgat gccctctacg cgaaaaagta catcacccgt acccaatatc
     3901 tcgaggtgaa ggatcgctac aacgctgttc tgcaggagat cctctccttc aacgaggatc
     3961 tggcccgact gccgatgacc gccgccgata ccacctcgca gatagagcgg cagcggattg
     4021 acgtggacat gcgccttctg acggcggaga agcagcttgc cgcgctcgag gagcgctacg
     4081 acctggagac ctccatccgc agtcccgagg ccgggcgcgt gctggagatt ttcaagctgg
     4141 aaggtcaggt catatccgcc ggggaggcgc tgctctcgct ggagaagaac gatgataccg
     4201 gcgcgccact gtacgtgcac gcctatgtcc agccgcagga cggcaaaaag atcctgccgg
     4261 agatggaggc gcagatttcg ccgtccgtgg tgaagcagga ggagttcggc gtgcttcgcg
     4321 gacgggtgac acaggtctcc tcgttccccg cgtccgcgag gggaatgacg cgggtgctgg
     4381 gaaacgaaca gctcgtccag gcactttccc gcggaggcgc gtccatagcc gtgacgatgg
     4441 agctctaccc ctcggacaag acggagagcg gctacgtctg gtcctccagg aacgggcccc
     4501 cgctggccct ccagagcggc accctctgcg gcgtgaccgt ggttgtccgc cgccaggctc
     4561 cggcgacgct ggtcctcccc ttcctgaaga agttctttct cggcgttgga gaacaagagc
     4621 gcgacggagg ggcgaaagat gcctgaccgc ccggcggcga aacaaacgcc ccctcccggg
     4681 ggggcttccc ttgccccttc ggggcaattc ccctcatgga aaaacagacg cgtcgttacg
     4741 ccgacaatgc tccagatgga ggtcaccgag tgcggggccg cctccctggg gatagtcctt
     4801 ggctactacg gcctccatct gcctcttgaa acgctgcgcg aggaatgcgg catcagccgc
     4861 gacggcagca aggcgaccaa ccttctcaaa gccgcgcgac gccacgggat ggaggcgaag
     4921 gggtaccgca aggaaccgca gactctcggc gatcttcctc ttccgattat ccttttctgg
     4981 aacttcaatc actttctcgt gctggaaggc atcaaaggca atagggtcta cctgaacgac
     5041 cccgcagcgg ggccgcgcac ggtggaccgc gagactctgg accgctcgtt taccggggtg
     5101 gccatttcca tcgtccccgg agagaatttt catcccgagg gccgtccccg cagcccctgg
     5161 gccgcgctcg gaagaaggct cgccggcgcg cgtacggcgc tgctcttcgc cgtgctcgcc
     5221 gggcttgcgc tggtgatccc cggcctcatg gaaccggcct tccagcagat cttcgtcgac
     5281 cgtctcctcg tcgaggggcg tcttgagtgg ttccgcccga tgctgaccct catggcgatc
     5341 gtcgcgctcg tcaagggtgc gctgacatgg ctccaggggt cggtgctgct ccgtcaggag
     5401 cttgcgatgg cgatccggga ttcagcctcc ttcatgaacc atctgctgaa gctcccctac
     5461 accttctttc tgcagcggta cgccggggag atcggcaacc gcgtgcagct caacgacagg
     5521 gtggcgcagc ttctctcccg ggagctggcg acgacggtgc tccagtgcat cctggtgctc
     5581 ttctacgggg tgatgattac cctctatgac cctcccgtct ccgtaatcgc ccttggcgcg
     5641 gcggcaggct gcgcgctgct catcatctcg gtgaacaggc ggcggaccga cctgaacgag
     5701 cgcttcaccg gcgaggtcgg aaaattcgcc ggggcggcga tcggcggtct ctccgtcatc
     5761 gagacgatca aatccttcgg cggtgaggag gaccagttcg cccgtctcgc ggggtacctg
     5821 agcaagatcg cggactgccg tatcgaactc tcccgcctcg cggtgcgaat tcaggccatc
     5881 ctgccgatgc tgaccgccct cgcagcggca ggggtgctct gctacggcgg ctttcgggtg
     5941 atggacggcg tgctcaccat gggtatgctg ctcgcgctgc aaacgctgat gacgcagacg
     6001 atcaacccgg tgacggagct ggtgaccctc tgggactctt ttcaaaccgc gcaggggaac
     6061 ctgaaccgtc tcgacgacgt gcttcgctac ccccgtatgc cggagcggcc ggcgctcgaa
     6121 gacggagcaa tggccgctcc gaaacatccg ggacaggcaa gggcacgttc agtgcgcctt
     6181 tccggacgcc tggagctgcg cgatgtctcc ttcggctact cgaagctcgg cgaaccgctg
     6241 atccgggact tttccctgac gctcgttccc ggctcgcgtg ttgcgctcgt cggcggttcc
     6301 ggctcgggca agtccacggt ggcccgtatt gtctgcggtc ttttcgaacc atggtcgggg
     6361 gaagtgctgc ttgacggccg cctgctggcg accatatcgc gggaagagac agcgacctcc
     6421 ttcgctttcg tcgaccagga gatctccctc tacgagggga ccatacggga gaatctttcg
     6481 ctctgggacc cctctgtgcc gacggagcag ctcgtggcgg cgtgccgcga cgctgctatc
     6541 cacaaggatg tcatggcgcg gcaggggggg tacgacggga tcgtcgagga gaacgggcgg
     6601 aacttcagcg gcgggcagcg tcagcggctg gagatcgccc gcgcccttgt ccgctccccg
     6661 tcgctgatcg ttctcgacga ggctacaagc gccctcgatg cccccaccga ggaggccatc
     6721 gaccgggcgc tgcgaaggcg tggatgcacc tgcctgatcg ttgcgcaccg tctgtccacc
     6781 atccgcgact gcgacgagat catcgtgctc catcagggga aggtcgcgga gcgggggact
     6841 catgaagaat tgctggggct cggagggcgc tatgcagggc tcattgagaa ctgagccgcg
     6901 cctcgaaaga ggcggattcc catggtaggc acccttccct tcgaggcgct ccgtcctctc
     6961 gggacggagc aggagattac cgggaaaact cccttcttcc tcgatgatcc ggagactgtc
     7021 tggtgtgtcc tctccgggca gattgatgtc tatgtgaccg cacgggaggg tgctctgcag
     7081 acggggggca gggatcatct cttcacgatg gaagcgggag aggccctttt cggggttgaa
     7141 cccggtcttt tcggggcgag catggggctc ctggcgggag gcattccggg aacaagagtg
     7201 ctgcggatct ccaggtccct tctcctcgaa caaacggacg gagagggaac cgcgattgcg
     7261 gcgttccttg ccgacaggtt tttggagggg ctggggcggg gcatcgtcaa ggatatcgta
     7321 ccgcttccgc gcataaaaca gtccattccc gccggaagga cggaactcgg gccgaacagg
     7381 ccggccggaa gtgaaacgct gctctggaca gagatggagg aaggccgcgc gcttttcgtc
     7441 ggcaccgagg accttgcgcc gggaagcgga ctcttcgccc ttcctccgaa ctgctggatc
     7501 gttcccctgg agcagtgcgt ggtgcggtcg attccaaccg gcgagctgct ccgccggggc
     7561 gagttcgccg cagtcttcga cgcgttctgc gaaatgatct tctcttcgct ggcgatgaac
     7621 gcgcggctcc gggccgcgga cgaccttgga gcgctccgcc agatgcagat gtccagcagg
     7681 agggcgctct cttcggccct cggaacgctg gcccgggcgc tttcagggaa acgccccagg
     7741 gagtttgcct ccagggagga gtcgccgatc cggcgagctt ttgccacggt cgccgttttt
     7801 ctggggcgga gcgccgtttt ccctcgaggc ggcgagattg ccgcagcgca aggagaagga
     7861 cgcgaatcgc tccgcgacct cgcacgtgca tccaacctcc agctccgccg cgtgacgctc
     7921 gccggaccgt ggtggacctc agacggcgcg ccgctgattg ccttcatgaa cgctcctggg
     7981 gacggcttgt ccggaagagg agacggaaga agcgggacgc ccgtcgcgct tcttccgtcc
     8041 ggacgcggct atcgtctcgt cgacgacggg gaggagcgtc cggtcgacga acagatcgcc
     8101 gcatccatcg cgccggacgc ttaccaggtc taccgtccgc tccccggctc gccgctgggc
     8161 ggcagggatc tgctccgttt cggcctcgcc ggggcgaaga gagacatggg ggcagtcctc
     8221 ctcatctctg ttttcctggc tctccttgga ctgatctccc ccgaggtgaa ccggcagatc
     8281 ttcggcgaag tcattcctca ggccgaacgg gggcggatgc tccagctctt cgccatactc
     8341 gtctcagtcg ctgtttcatc cggtctgctg cacctggcga acgggacggc cttcctccgc
     8401 accgagagcg tgatggacca cgacgtctcc gcagggatct gggaccgcat acttcgcctg
     8461 ccggtaggat tcttccggaa aacgacgtca ggcgacctcg caaaccgggc ccttggcctc
     8521 ttcatgctcc gcgagaccgc gtcgaggacg gtgaagacgc tgctcgtcca gagcgtgttt
     8581 cttgtttttt tcctcggaca gtctttctgg tacgactgga ggctggcgct cgccggactc
     8641 ggatgccttc ttatcccggc agtgctgatg gtggcggtga accttttcca gcttcgtttt
     8701 cagcggaaga tgatcggcct gcagaaccgg ctcctggcgc tgacgttcca gattatgacc
     8761 ggcatttcaa agattcgcgt cgcgggcgcc gaagagcgtt ccttcgcccg ctgggcggag
     8821 ttgttcggag cgcagcggat gctggggacg aaggcgagac ggctggaagt ttttctgaac
     8881 acggtcatgg cactcttccc cctctccgtc gccatcgtta ttctctttgc ccttgtccgc
     8941 tggagcgctg cggacgcctc gtacgaaacg gggcgcttca tggccttctg gtcggccttc
     9001 ggggcgctgc aaggggcctt ctttcagatg gtcagctcgt ttacagccac gctgaacatg
     9061 cttccgctgg tggagaacct cgcacccatc ctccgggcgg agcccgagac cggcgacatg
     9121 aagaccgacc cgggcgaaat cacaggtcgt atcgaccttg aaggggttgt cttccgctat
     9181 ctgaaggacg gccctcccgt gctgaaagga cttgacctcc acattgaggc aggggagttc
     9241 gtcgccgtgg tcggtccctc cggggcgggc aagtccacgc ttatccggct gctcctgggt
     9301 ttcgaccagc cggagtcggg aggcatcttc tacgacaaca aggacattgc cgaactggac
     9361 ctggggaaac tgcggcggca gatcggcgtc gtcctccaga gcggcggact gctctccggg
     9421 gacatagcct ccaacgtgcg ctgctcgcgc aatctcacgg ccgggcagat agaggatgcg
     9481 cttcgcacgg cggggatgga agaggacatc gccgcgatgc ccatggggat tcatacggtc
     9541 atcacggacg gggcgaacac ggtttcaggc ggacagaagc agcgcctcct gatcgcccgc
     9601 gccgttgccg ggaagccgcg tgtgctcatc ttcgacgagg ccacgagcgc cctggacaac
     9661 agaacgcagg cgatgatcag ccggagcctg gagaacatcc aggcgacgcg catcgtcgtt
     9721 gcgcaccggc tctccactgt catcggcgcg gaccggatct gcgttctgga ggacggccgg
     9781 ctcgtcgagg aggggaagta cgacgagctg atggcgaagg acggtctttt cgcagagctg
     9841 gtccgcaggc agacggtata gaatcttgtc cgcttccccc ggaagaacgg gagggttctg
     9901 tgctttttcg gaatgccgga tgcgcttcgg cccgtatgcc tgttcttttt ggctacacca
     9961 cgaaatgggt cagccagtat ttagaatcaa aacgatgcag catatcgatg agctgccgat
    10021 ggaattccct ctcgttttca tacgagccgc cgaacacctc ataaagctcg gtctgaattt
    10081 tcttcaggct tcgtttgccg gcgcagcgca acagcagttc atactccagc ggcgagagcg
    10141 ccataccgtc cagctgcgga aatccgtcgg aaaacgtcat ggcgcgccat aattcaaagg
    10201 tccgctgggg ataatcccgg gaaaaatcca gggattcctc aaaggcttgg ccctcgttca
    10261 aaaaaagcac ataataatat tcgtaggccc tgggattggc cgaaatttcc gcgaaccacc
    10321 ggcttcggat cccgtatttg aatgcggctt cgaactgctt cctcaccgcg tcctctttca
    10381 gcccgcggtg tttgattttt tgctgcatgc tgcggcgaat caccctgttg agttcctggc
    10441 gctggcgcat cacctggtcc tggctgcatc cgaccggcgt tacgaacggg tagtcgtccc
    10501 cggtccggcg gccggattcg tcatggagct gcaggttgaa ttttcccggg ttctcgaaga
    10561 tctcggtccc cgggtaggcc cgtaaaaaac cggtaatgat atcgatgact ccgggggccc
    10621 gatcgatcag ggtccggatc agcgaggcgg tcgccccgtc tttttctgcg ggccccccca
    10681 cgatgaagtt tgtggcgatc tgggcgaggc cgcagtcggc cgcgtaggtt acaaattcaa
    10741 cgatgtcgtc gattttgacg tgcttgttgt actggtcgat gacgttctga tcacctgatt
    10801 ctatcccgat ctgcagacgg atcagcccgg cgtcgatcat ggcgggcagg atatccataa
    10861 agtccctgat tcggccgaca tggcattcac agtaccagac caggtcacgc tctttccgca
    10921 gctccgtcat gccgcggcag aattcttcga tccgctcccg gccgagggta aaagtgtcgt
    10981 ccataacgat caggcacttc agccccggat tgtttttcag attgactttg atttcctcca
    11041 gcaccagcgg aatgcttctc aggcgaacct tccgcttgcc ggggctgggg gcgcagtatg
    11101 cgcaccggta gggacacccc cggccggtaa aaatcaagtt gccgtaggcg tggtccgcat
    11161 gcagcgatgc tgagtacgcc ggcaggggca gagcatccag atcctcgatg atctcgggcg
    11221 ggttttccag aaacactccg tcctgaattc tcgccagccc cttcaccccg tcgacatccc
    11281 cttcatcctt caggaccgca tcgagcagcc tgggcagaga gacttcgcct tcgccccgca
    11341 ggatatagtc agccccgctt ttttccagaa aatcccttcc cagagcaatg ctctggggcc
    11401 cgcccacaac aacgggaatt ccccagcgct cctttatttt ccctgacatg tcttcgatga
    11461 tccggacatt gtcgaaatca caggaaaaac ccaccgcccg gggcttgttc ttttctatct
    11521 cgtccaggag atgttccggc tttccatgaa agaccaaagg ctcaaagccg gcctcttcca
    11581 gggcgcccgc caggtaaaag agccccacat aatcatgggc attggtatcg gactttttcg
    11641 acacgttgca gagaaagatc ttcatcgcat caccttttct agtacggaac gacaagcagc
    11701 cagtactttt catcaaaaac cttcaaggtt tcaatgattc gctccatcaa ttcttcctgc
    11761 gtttccccga acggttttcc gaaccgctcg tggaggatcc tggcaatggc ggcaat
//
LOCUS       DAOPKU010000019        11732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_5742_length_11732_cov_4.818361, whole genome shotgun sequence.
ACCESSION   DAOPKU010000019 DAOPKU010000000
VERSION     DAOPKU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5742_length_11732_cov_4.818361"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /gene="mrdA"
                     /locus_tag="PK849_01190"
     CDS             complement(<1..1627)
                     /gene="mrdA"
                     /locus_tag="PK849_01190"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPE64766.1"
                     /translation="MSDHRPEMNLRLLLWKLFLMFSLLLLAGGLYFFQIVNADAYVRL
                     AANNRLRFIRFAPIRGEIFDRNGVPLAANVTTFDIMGYPLDIEKNNMVENLALLLSRH
                     GIPVSAEDLRRDVKRQFWAPYRVVRVVSNITLAQMADLVADPEFPRQLFPIPVLRRTY
                     PAGSIASNVTGFVGEINENELRQMEGKSYVGGDQIGKTGIERQYEEILRGEPGGESVE
                     VDARGRRVRQIDTYPAGRGEDLHLTLDLGAQRLAADLLQGYRGALIVMDVHDGDVLVL
                     ASSPSYDNNPLSWGVSAKEWNALLSDPQKPMLDRSIAGLYPPASTFKILVGLAALVEK
                     EVTPSTTFHCGGAFTLGNRTFRCWRRSGHGTVNLTGALQNSCDVYFYQVGLKLGITRL
                     LKWVELFGLGRPTGIDLPGEAGGVVAGPDWKKARFKEGWYRGDTVNYSIGQGFLLTTP
                     LQLATMYAVIANGGKLVRPRLANSLPVQAAELNLSPSHMNVIRKGLDYVVRRGTARRA
                     GGFGITVAGKTGTAQNAHGDDHALFVGYAPMDNP"
     gene            complement(1620..2078)
                     /locus_tag="PK849_01195"
     CDS             complement(1620..2078)
                     /locus_tag="PK849_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64767.1"
                     /translation="MIYLLAWYAQDFLWVLGAGKVLVPEIFLLTLVFLSLFRSEDGVR
                     VVWAAFLGGVLWDLRWTGFPGISSLIYVLSLMSVRWIWHSIPASGRTITLFGFLLWSA
                     NMPIALGRIFLWGARGIEVWRAYLLQQSHIVPLVLLACAAYAWRLKNQDV"
     gene            complement(2075..2893)
                     /gene="mreC"
                     /locus_tag="PK849_01200"
     CDS             complement(2075..2893)
                     /gene="mreC"
                     /locus_tag="PK849_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPE64768.1"
                     /translation="MTEKYSEREWIHGLLALAASLLLLAAAPRREFFRGAVDFVGEVL
                     RYPEYPAVFIERTTREFSVWFADRYVLQERLNTLKEENERLNLVWSMENARNLRRELD
                     GFVRYARITLREPLSWWAEVRINKGVHDGVVVGAPALQSGFLAGRVSGAEDDFAWVEL
                     LSSSSLMIPVVIEETRDLGVVAGDGEGGVWLLYVPENRMLKSGMRVSTAMVSEALPPG
                     IPLGSLGEERRETSGGYTEWRILPGASLSQLYALEVLGLRTPSQEKPAGGRSGL"
     gene            complement(2921..3976)
                     /locus_tag="PK849_01205"
     CDS             complement(2921..3976)
                     /locus_tag="PK849_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPE64769.1"
                     /translation="MFKFLTGVFGGDVGIDLGTANIVVYVKGRGIVISEPSAVAIRKR
                     PRGGATEVIAVGREAKAMAGKTPGGVFTIWPLQDGVIANFEMTEELIRHCLRRANGGK
                     PFVSHPRVVISVPAEVTEVERKAVIDATLGAGAKEAYVIEEPVAAALGVGLPIQEPRG
                     SMILDIGGGTSEVGVLSLGGIVVTNSLRAAGKEMDNAIVAMLRQRYALLIGDTTAEEV
                     KIAIGSALPLDKELEMAVKGRDLADGLPKADVVTSVEVREALEPILLRIEDMVKVALE
                     QTPPELAKDIVDQGIFLSGGVSLLRGLAERLSKAMNTPVVVAEDPLFAVARGVGKILE
                     NLGDMKKVLLSVEKGSK"
     gene            complement(3996..4667)
                     /gene="radC"
                     /locus_tag="PK849_01210"
     CDS             complement(3996..4667)
                     /gene="radC"
                     /locus_tag="PK849_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPE64770.1"
                     /translation="MKLASLPREEMPRERLRLRGASSLSLPELLAILLRTGSRGKDVL
                     ELAADVLNEFGGAKGMARATEEEMLGFPGLGDAKACVLLAALELGKRLEAARRELQEG
                     KDWRRSVDEIAGELAFEEREYIFAIHVRSNGSVIHVDRISFGGAEGAYLDVKYLLRRA
                     VRMDSYGLVLVHNHPDGSLQPSREDRLLTDFVSRRTKILDIHLLGHFVVANGESHGIA
                     LPGEP"
     gene            complement(4664..5314)
                     /locus_tag="PK849_01215"
     CDS             complement(4664..5314)
                     /locus_tag="PK849_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE64771.1"
                     /translation="MKYTRFPLGPLWTNSYLVWDASGDGFLVDPAGDAAEILSFAREN
                     SIVVRAVLLTHGHLDHVGGLPELLEKNASPVYIGEGDAEMLRSPDQNAAEWMGYDFKG
                     IRNFFTLRDGEQLSVGVMSVSVLETPGHTPGCVCYHVSESSDTGEETLLLSGDTLFAR
                     SVGRTDLPGGNGERLHESLIRLAALPDTLRVLPGHGPETTIGAERIHNPFWPEDKA"
     gene            complement(5423..5860)
                     /gene="dtd"
                     /locus_tag="PK849_01220"
     CDS             complement(5423..5860)
                     /gene="dtd"
                     /locus_tag="PK849_01220"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPE64772.1"
                     /translation="MRAVVQRVSSARVSVDGETKGAIGKGVCVLLGVSDADGEQEADW
                     MAEKIVNLRIFEDDEGKMNRSLLEVGGSMLAVSQFTLYGDCRKGRRPSFVDAGEPGHA
                     NRMYESFKSAAAGRGVEVESGVFQAHMLVEIANDGPVTLIIDR"
     gene            complement(5868..8243)
                     /locus_tag="PK849_01225"
     CDS             complement(5868..8243)
                     /locus_tag="PK849_01225"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPE64773.1"
                     /translation="MDPSKLTESILESRTHPVRTGALPDQDPAAESGEVSSLRSGGTA
                     DLDRKSSGKLRDSYIGRIPEEERAISVKFAWHELWSKVIVYLSREELMQLGEALVMAG
                     AAHKEQRRSTGDPYIVHTINVASILADMQLDNVTLMAALLHDVLEDTDITMEKLTGAF
                     GAPVAMLVDGVTKLGKLPFKSFEDYQAENLRKMFVVMAKDIRVVLIKLADRLHNMRTL
                     GALRKDKQQRIARETLEIYAPLAHRLGIYQVKRGLEDLAFKYADPEMYYEIRRRVRKK
                     LPERETVVKKALEVLTARLEEEGIRCKVKGRAKHFYSIYEKMNRKKLPVEQLYDLLAL
                     RVMVDDIAACYTVLGIVHTIWKPIPGQFDDYIANPKSNMYQSLHTTVVGPTGEPLEVQ
                     IRTWDMNSLAEYGIAAHWRYKEGGASLDDLDAKLTWIRQAIEADHDGSEPSEFLERLK
                     EDVLSSDVFVFTPQGEVVSLPKGSTPLDFAFAIHTQVGTRCVGAMVNNRIVAMGYELQ
                     NGDIVKIITSPQGAPSRDWLKIARSSKARSKIRAYFRALEKTERLEKMQRGRDLLERE
                     VKRRGTPEFEEFDELLPLLNKIARDTAYSNGEELLSAIGGGHQSVSLVAQRLAGKPQP
                     QPAPVQSLPPEPEKHTSFSGKKGDSDIVVEGADGVQVVLSNCCEPVPGDPIIGFSTRT
                     RGITVHRADCENVRNSREERRVSVSWGMTGTRRFSARLKIEAVDRAGLFGDVAQAITA
                     GDGSMTGIKAGVVGGNQARMKLEVRVRDLEHLYLIVARLNAVKGMIQVSRG"
     gene            complement(8303..10021)
                     /locus_tag="PK849_01230"
     CDS             complement(8303..10021)
                     /locus_tag="PK849_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPE64774.1"
                     /translation="MTALCRSASLRLFQPDSSTVELADRIGCSLLTAAVLESRNLAAA
                     DERAPSLPDASGVESARESLFLDAKIPFEDLMEELSLGENAAEAADRWRSLKTLGRVL
                     VYGDYDADGVSSAALALELCRGKAERARFFIPHRHEQGYGLHESVLDQLLDIGWDTLV
                     VVDCGTKDGEILEKVARSGVNVFVFDHHLPEEGAPLHSAVVNPHTCAPRSAPGENEAG
                     RALCATGVLWAWAWKHSLLPRKRLAAMTDLVALATIADCMPLTPLNRALVRQGMDILS
                     TTPRKGLARLFSRLGIARPGSALTEETLSMKVIPCINAAGRMDFADTAVNVLLGSPNV
                     DESVEMLISLNRKRQSLSLKISKEAELVLSGGVNVILGESWPVGVLSGVASRICGATG
                     APVALAAPVNGGIRGTLRVPEGADAVGVLSALSSKLEAWGGHKYAAGFSVSRENWDFV
                     RRDLQQALSSMKVEETSVTAIAMAPEEISIESWKEACTLGPFGNGNPPPMFFLARKGR
                     ERMLPLGKDGKHIQIDAGGIRLLAFHGENEREALAGADGWVYRPRIDCWRGEERLQYV
                     VDYAVV"
     gene            complement(10351..>11732)
                     /locus_tag="PK849_01235"
     CDS             complement(10351..>11732)
                     /locus_tag="PK849_01235"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HPE64775.1"
                     /translation="PVLKNKGGIWIGWAGTHENVSLSTLREILGPVSETSGYRLLPVM
                     LTKEDVDSFYSGFSNSVLWPLFHDLQSRCDFNPSYWDGYLAANRKFAKVLMKHTEEHD
                     FIWVNDYHLIPLGKLLREKKPSLKASFFLHIPFPSVDIFMKMPQRKEILSQLIHYAFV
                     GFQTMRDRRNFLDCLKVFFPEMQVSGRGNVVRVRIGESNFLVGALPISIDYRSFASLA
                     SSKEVISRSKDIQREMFAQKYILGVDRLDYSKGIPERLRGFQRCLERYPELREKLSLM
                     QVVVPSRESVGEYLDLKQEIELLISRINGEYSTMRWVPVHWRYGSVPRSELAALYRGA
                     EMALVTSLKDGMNLVCKEYCACKGDGSGVLVLSEFAGAAIQLSKGALLVNPYDVDAVA
                     AAIAEGFAMDAHEKKRRMRLLRKSIARHDVFRWVDDFLRASVGKKLAEFPEEEIPPLW
                     PGLESDGEK"
BASE COUNT         2330 a         3820 c         3266 g         2316 t
ORIGIN      
        1 tcgggttatc catcggcgcg taccctacga agagcgcgtg atcatccccg tgcgcgttct
       61 gagcggttcc ggtctttccc gcgaccgtaa tgccgaatcc tcccgccctc cgggccgtcc
      121 ctctccggac gacatagtcc agccccttgc gtatgacgtt catatggctc ggtgaaaggt
      181 tcagttcagc cgcctgcacc ggcaaactgt tcgcgagccg cggccggaca agcttgcctc
      241 cgttggcgat cacagcgtac attgtggcga gctggagcgg cgtcgtcagc aaaaacccct
      301 gcccaatgga gtaattgacg gtatcgcctc tgtaccagcc ctccttgaaa cgggccttct
      361 tccagtcggg gccggccacg acgccaccgg cctctccggg aagatcgatt cctgtgggcc
      421 tccccaatcc gaaaagctca acccacttca aaaggcgggt aatcccgagc ttcagtccaa
      481 cctggtaaaa atagacgtcg caggaattct gaagcgcccc cgtcaggttc accgtcccgt
      541 gtcccgaacg tctccagcag cgaaacgtac ggtttcccag ggtgaaagcg cccccgcagt
      601 ggaaggtcgt cgacggagtg acctccttct ctacaagagc ggcaagcccg acgagtatct
      661 tgaaggtcga cgccggcggg taaagcccgg cgatactcct gtcgagcatt ggcttctgcg
      721 ggtcggagag gagcgcattc cactccttcg ccgaaacgcc ccacgagagg gggttgttgt
      781 cgtacgaagg ggaagaggcc agtacaagga cgtccccgtc gtgcacgtcc atcacgatca
      841 gcgctccgcg gtatccctgg agaagatcgg ccgccaggcg ctgcgccccg agatcgagcg
      901 tcaggtgaag gtcctcgccg cgccctgccg gataggtatc tatctgccgg accctgcgcc
      961 ccctggcgtc cacctcgacg gattctcctc cgggctcccc gcgaaggatc tcctcgtatt
     1021 gcctctcaat accggtcttc ccgatctggt ctcccccgac gtagctcttc ccctccatct
     1081 gacgcagctc gttctcgttg atctcgccga caaatcctgt gacgttcgac gcgatacttc
     1141 ccgccggata ggtccttcgc aggacgggga tcggaaagag ctgccgcggg aactcgggat
     1201 cggccaccag gtcggccatc tgcgcgaggg ttatgttgga gaccacgcgc accaccctgt
     1261 agggcgccca gaactgccgc ttgacatccc ggcgaaggtc ctccgccgat accggaatac
     1321 cgtggcggga gagcaaaagc gcaagattct ccaccatgtt gttcttttcg atatcaagcg
     1381 ggtaccccat tatatcgaat gtagtcacgt tcgccgccag cgggactccg ttcctgtcga
     1441 aaatctctcc ccgtatgggg gcgaaccgga tgaaccggag gcggttgttc gccgcgaggc
     1501 gaacgtacgc gtcggcgttg acgatttgaa aaaagtaaag ccctcccgcg agcagaagga
     1561 gcgaaaacat aagaaacagc ttccacaaaa gaaggcgaag attcatctcc ggacgatggt
     1621 cagacatcct ggtttttcaa cctccacgca taggcggcgc aggcaaggag caccaacggc
     1681 acaatgtgac tctgctgaag caggtacgcc cgccacactt ctattccccg cgctccccac
     1741 aggaaaattc ttccgagcgc gataggcatg ttcgcggacc agaggaggaa accgaagagc
     1801 gtgatcgtcc ttcccgaggc cgggatggaa tgccaaatcc accggacgga catcaatgac
     1861 agaacgtaaa tcaaggagct gatacccgga aatccggtcc atcgtaaatc ccagaggacg
     1921 cctccgagga aagcggccca aaccacccga actccgtcct cggaacgaaa gagcgaaaga
     1981 aagacgaggg tgagcaaaaa aatctccggg acgaggacct tcccggcgcc gagaacccac
     2041 aaaaaatcct gagcatacca cgcgagcaga tagatcataa accggacctc cccccggccg
     2101 gtttctcctg cgatggagtg cgcaggccga gaacttcgag cgcatagagc tgcgagagag
     2161 acgcgcccgg aagaatccgc cactcggtat atccgccgct cgtttcgcgg cgctcttcgc
     2221 cgaggctgcc caatggaatc ccgggaggca acgcctcgct gaccatggcg gtgctcacgc
     2281 gcatcccgct tttgagcatc cggttctccg gcacgtacaa cagccagact cccccttcgc
     2341 cgtctcccgc cacgacgccg agatcccgtg tctcctcgat aaccacagga atcatcaggg
     2401 atgatgacga gagcagctcg acccacgcaa aatcatcctc cgcaccgctg actcttccgg
     2461 cgagaaatcc gctttgcaga gcgggggccc cgacaacgac gccgtcgtgc acccccttgt
     2521 tgatccgcac ctcggcccac catgagaggg gttcccgcag cgttatgcgg gcgtagcgga
     2581 caaagccgtc aagctctctc cggagattgc gcgcattctc catcgaccag acgagattca
     2641 ggcgctcgtt ctcctctttc agggtattga ggcgctcctg taaaacgtaa cggtcggcaa
     2701 accagacgga gaactccctc gtcgttctct cgatgaagac ggcgggatac tcggggtagc
     2761 gcagaacctc tccgacgaaa tcaacagccc cccggaaaaa ctctcttcgg ggagccgccg
     2821 caaggagcag aagactggcg gcaagtgcca aaaggccgtg aatccattct ctttcgctgt
     2881 atttttccgt catgagcagg ctctccgccg gatgcgcaga ttacttggac cccttctcca
     2941 cggagaggag caccttcttc atgtcgccga gattctccag aatcttcccc acgccgcggg
     3001 ctaccgcgaa gagaggatcc tccgccacca ccacaggggt attcatcgcc ttggacaggc
     3061 gttcggccag acctcgcagc agcgagacgc cgccggacag aaagatcccc tggtcgacga
     3121 tgtccttggc cagttcggga ggcgtctgtt cgagcgccac cttcaccatg tcctctatgc
     3181 gcagcagaat cggctccagc gcctcgcgca cctccaccga ggtcacgacg tcggccttcg
     3241 gaagcccatc tgcgaggtcg cgccccttga cggccatctc aagctccttg tcgaggggaa
     3301 gcgcggatcc gatggcaatc ttgacctcct ccgccgtcgt atcccctatg agcagcgcat
     3361 atcgctggcg gagcatggcg acaatggcgt tgtccatctc cttcccggcg gcgcggagcg
     3421 agttggtcac gacgatgccg ccgagagaga gaacgccgac ttcgctcgtc cccccgccga
     3481 tgtcgaggat catgctccct cgcggttcct ggatcggcag tcccacgccg agcgccgccg
     3541 caacaggctc ctcgatgacg tacgcctcct tggcgccggc cccgagcgtt gcgtcgataa
     3601 cggccttccg ctcgacttcg gtaacctccg cgggcactga gatcacgacc cgcggatgag
     3661 agacgaaggg tttccccccg ttggccctcc tgagacagtg acggatcagt tcttcagtca
     3721 tttcgaagtt cgcgatgacg ccgtcctgca gcggccagat agtgaagacg cctccgggcg
     3781 tttttccagc catcgccttg gcctcgcgtc cgacggcgat cacttccgtc gcccctcctc
     3841 tggggcgctt ccgaatagca acggcggacg gctcgctgat gacgattccc cgccccttga
     3901 cgtacacaac tatgttggca gtcccgaggt cgataccgac gtcgccgccg aacactcccg
     3961 taagaaattt gaacacctgc ttcagtcccc gctttctagg gctctccggg gagagcgata
     4021 ccatggctct caccgttggc cacgacaaaa tgtcctaaaa ggtgtatatc caggatcttt
     4081 gtcctcctcg atacaaaatc agtcagcaga cgatcctcgc ggctcggctg cagcgacccg
     4141 tcgggatggt tgtgcaccag caccaggccg tacgaatcca tccggacagc tcttcgtaac
     4201 agatatttta catcaagata ggcgccctcg gcgcctccga aggaaattct gtccacatga
     4261 atcacggatc cgttgctccg gacgtgaatg gcaaagatat attcccgctc ctcgaaagcc
     4321 agctctccgg ctatctcgtc gacgctccga cgccagtcct ttccctcctg aagctctctc
     4381 ctcgccgctt cgaggcgctt tccaagctcc agggcggcaa ggagaacgca ggccttcgcg
     4441 tcgccgagtc cgggaaaacc gagcatctct tcctccgtag cccgcgccat tccttttgcg
     4501 cctccaaact cgttgagcac atccgcagcg agttcgagga catccttccc cctgctcccg
     4561 gttctgagca gaatcgcaag cagctccgga agcgagagcg acgaggcgcc gcgaagtcga
     4621 agccgctccc gcggcatctc ctcgcggggc agcgaggcca gtttcacgcc ttgtcctccg
     4681 gccagaacgg gttgtggatg cgttccgctc cgatagtggt ttcgggaccg tggccgggca
     4741 gcacccggag tgtatccgga agcgcggcca ggcgaatcag cgattcatgc agtctttccc
     4801 cgtttcctcc gggaaggtcc gtacgcccga cgctgcgggc gaagagggtg tctccggaga
     4861 gaagaagggt ctcctctccc gtatccgagg actccgatac atgatagcag acgcatccgg
     4921 gcgtatgccc cggggtctcc agcacggaga ccgacatgac gccgacggaa agctgttcac
     4981 cgtccctcag cgtaaaaaag ttcctgatcc ccttgaaatc ataccccatc cattccgccg
     5041 cgttctgatc gggtgagcga agcatctccg cgtcgccctc tccgatgtac accggggaag
     5101 catttttctc aaggagttcg ggcagcccgc cgacgtggtc aagatgtccg tgagtgagga
     5161 gaaccgcgcg gacgacgatg ctgttctccc gtgcaaacga gaggatttcc gccgcatcgc
     5221 cggccggatc aaccagaaag ccgtctcccg aagcgtccca tacgagatac gagttcgtcc
     5281 agagaggccc caggggaaat cgtgtgtact tcatcataaa tcccttcctt ttgtacgcat
     5341 acttcccggc gccgcgctat gcggccgcag gttcttttcg cgaagggggg cgcactgcgc
     5401 gccttcgccc tccgcacatg actcagcggt cgatgataag cgtcaccgga ccgtcgttcg
     5461 caatctctac gagcatgtgc gcctggaaaa cgccgctctc cacctcaacg cccctcccgg
     5521 ccgccgcgct cttgaacgat tcgtacattc gattcgcgtg accgggctcg cccgcatcga
     5581 caaacgaagg ccgtcttccc ttccggcagt ctccgtagag cgtgaactgc gagaccgcaa
     5641 gcatgctgcc gccgacttca agcagcgacc tgttcatctt cccctcgtca tcctcgaaga
     5701 tacggagatt gacaatcttc tcggccatcc agtccgcctc ctgttcgccg tcggcgtcgg
     5761 aaacgccgag caggacgcac actcccttcc cgatggctcc ttttgtctct ccgtccacgg
     5821 aaacccgtgc ggaggagact ctttgcacca cagccctcac gacagcgtca cccccgcgat
     5881 acctggatca tgccctttac ggcattgagc ctggcaacta taagatataa gtgttcaagg
     5941 tcgcggacac gaacttcaag cttcattcgc gcctgatttc ctcctacgac tcccgccttg
     6001 atgcccgtca tactgccgtc ccccgcggta attgcctggg cgacgtcgcc gaagagcccg
     6061 gccctgtcca cagcttctat tttcaggcgc gcggagaacc tccgggtacc cgtcattccc
     6121 cacgacacgc tgacgcggcg ttcttcgcgg gagttcctga cgttttcaca gtcggccctg
     6181 tgcacggtga tccccctggt tcgcgtggaa aagccgatga tcgggtctcc gggaaccggc
     6241 tcgcagcagt tggaaaggac gacctggacc ccgtccgccc cctcaaccac gatatcggaa
     6301 tcccccttct tcccggaaaa cgaggtatgc ttctccggtt cggggggaag ggattgcacc
     6361 ggagcaggct gcggctgcgg ctttccggcg aggcgctgcg ccaccagcga gacgctttga
     6421 tggccgccgc ctattgcgga gagcagctct tccccgtttg aataggccgt gtcgcgggca
     6481 atcttgttga gcagcgggag cagttcgtcg aactcttcga actcgggagt ccctctcctc
     6541 ttgacttcgc gttcgagcag atcccggccg cgctgcatct tctccaggcg ctcggtcttt
     6601 tcgagggcgc ggaagtaggc gcgtatcttg ctcctggcct tgctgctgcg ggcgatcttg
     6661 agccagtctc tcgaaggggc tccctgcggc gacgtgatga tcttgacgat atcgccgttc
     6721 tgcagctcgt accccatggc gacgatacgg ttgttcacca tcgcgccgac gcatcgcgtc
     6781 cccacctggg tatggatggc aaaggcgaag tcgagcggcg tcgacccctt cggaagcgaa
     6841 acgacctcac cctgaggcgt gaagacgaag acatccgaag aaagcacgtc ctccttcagg
     6901 cgctccagga attcggacgg ttccgagccg tcatgatccg cctcgatcgc ctgacggatc
     6961 caggtcagct tcgcgtcgag atcgtcgagg gaggcgcctc cctctttgta tcgccagtgc
     7021 gctgctattc cgtactcggc cagggagttc atatcccagg tgcggatctg cacttcaagg
     7081 ggttctcccg tcggtcccac cacggtggtg tggagcgact ggtacatgtt cgacttcgga
     7141 ttggcgatat agtcgtcgaa ctgccccgga atcggcttcc atatcgtgtg gacgatgccg
     7201 agcaccgtat agcatgcggc gatatcgtcc accatcaccc ggagcgcaag caggtcgtag
     7261 agctgctcca cggggagctt cttcctgttc atcttttcgt agatactgta aaagtgcttc
     7321 gcgcgcccct ttaccttgca gcggatccct tcctcttcaa gccgggcggt caacacttcg
     7381 agcgccttct tgaccacggt ctcccgttcg gggagttttt tccggacgcg gcgtctgatt
     7441 tcgtagtaca tctcgggatc ggcgtacttg aatgcgagat cctcaagtcc gcgtttcacc
     7501 tgataaatac cgaggcggtg cgcaagcggc gcatagattt cgagcgtctc gcgcgcgatg
     7561 cgctgctgct tgtccttcct gagggctccc agagtgcgca tgttgtggag acgatccgcg
     7621 agcttgatga gcaccacgcg gatatccttg gccatcacga caaacatctt ccgcaggttt
     7681 tccgcctggt aatcctcgaa cgacttgaag gggagcttgc cgagctttgt caccccgtcg
     7741 accagcatcg ccacaggagc gccgaaagcg ccggtcagtt tctccatggt gatgtcggta
     7801 tcctccagaa cgtcgtgaag cagggcggcc atcagcgtga cgttgtcgag ctgcatatcc
     7861 gcaagaatgg aagcgacgtt gatcgtgtgg acaatgtacg gatcgccggt cgagcggcgc
     7921 tgctctttgt gggcggcgcc cgccattacg agcgcctcgc cgagctgcat cagctcttcc
     7981 ctggagagat agacgatcac cttcgaccag agctcgtgcc atgcgaactt gaccgatatt
     8041 gccctctctt cctccggaat acggccgata tagctgtcgc ggagctttcc cgagctcttc
     8101 ctgtcaagat ccgccgttcc cccggagcgc aacgaagaaa cctcgccgct ctccgctgcg
     8161 gggtcctggt ccggcaacgc tcccgtacga acgggatgcg tccggctttc cagaatactc
     8221 tcggtcagtt tactggggtc catttttccc tccgctcaca tctggaaccg atcgcttcca
     8281 aagaactccg gggtgcgcct tgctaaacaa cggcgtagtc gacaacgtac tgcagacgct
     8341 cttcgcctcg ccagcaatcg atccgggggc ggtacaccca tccgtccgct cctgcgagcg
     8401 cctcccgctc gttctccccg tgaaaagcga gaaggcgaat cccgcccgcg tcaatctgga
     8461 tatgtttgcc gtccttgccg agcgggagca ttcgctccct tcccttgcgg gcgagaaaaa
     8521 acattggagg cgggttgccg ttgccgaatg gaccgagcgt gcacgcttcc ttccaggact
     8581 ctatggatat ctcctccggc gccatcgcaa tcgccgtcac agacgtctcc tcgaccttca
     8641 tcgaggaaag cgcctgctgg agatcgcgcc ggacaaaatc ccagttctcc ctggagacgg
     8701 aaaaaccggc ggcgtacttg tgccctcccc acgcctccaa ttttgaagag agggcggaga
     8761 gcacgccgac ggcatcggcg ccttcaggaa cgcggagcgt tcctcgtatg ccgccgttca
     8821 caggggccgc aagcgccacc ggagctcccg tggcgccgca aatcctgctg gcgacgccgc
     8881 tgagcacccc gaccggccag gactccccga gtatgacgtt cacgcctccg gagagcacca
     8941 gctccgcctc tttcgatatc ttcagcgaaa gcgactggcg cttccgattc agcgaaatca
     9001 gcatctccac ggattcatcc acgttcggag aaccgaggag cacgttcacc gcagtatcgg
     9061 cgaaatccat cctcccggcg gcgttgatgc agggaatcac cttcatcgag agggtctcct
     9121 ccgtcagcgc gcttccgggg cgggcgatcc cgagtctcga aaaaagccgt gcgagcccct
     9181 tgcggggggt tgtcgagaga atatccatac cctgccggac aagcgccctg ttgagcggcg
     9241 tcaacggcat gcagtccgct atcgtggcga gcgcgacgag atccgtcatc gccgcgagac
     9301 gttttcgggg aaggagcgag tgcttccatg cccatgccca gagcactccg gtagcgcaca
     9361 gcgctctgcc ggcctcgttc tcccccggcg ccgaccgggg cgcgcacgta tgcggattca
     9421 ccaccgcgct gtgaagcggc gccccctctt cgggaaggtg gtggtcgaag acgaagacat
     9481 tgacgcctga acgcgcgacc ttctccagga tctccccgtc cttcgtgccg cagtccacca
     9541 cgacgagcgt atcccagccg atatcgagga gctgatcaag caccgattcg tgaagtccgt
     9601 acccctgctc atggcgatga ggaataaaaa accgggcgcg ctcggccttc ccacggcaaa
     9661 gctccagggc aagcgcagcc gacgacaccc cgtccgcgtc gtaatcgccg tacacaagca
     9721 ccctgccgag cgttttgagg ctccgccagc gatctgccgc ttcggcggcg ttctccccaa
     9781 gagacagctc ctccatgagg tcttcgaacg gtatctttgc atcgagaaac agactctcac
     9841 gggcactctc cactccggaa gcgtccggaa gagatggggc ccgctcatcg gcagcggcca
     9901 ggttgcggct ctcaaggaca gccgctgtaa gcaacgaaca gccgattctg tccgcaagct
     9961 ccacggtcga ggagtcgggc tgaaagagac gaagcgaagc ggaacgacag agcgccgtca
    10021 caggactccg ttccctttcc gttccgcact gtttttttgg acgcaccgta cgccgttccg
    10081 ggcccggatg tcattcgcac atctttctgc tgtctgaaaa tacaccataa atacatcctc
    10141 atttcaccat atatcgtaac ggaagacgtg cattactcca tcttccggcc ggccgtctgt
    10201 tttccgtgcg tgtattccgg gcggagctct cagcggcgct ccgcagaaaa ctgcggagcc
    10261 tgctcacgat gaaccgtgca gctccgcttc atttctatcc ttttgtttat tgtagtcggt
    10321 gaggatggag aagtaaactc ccgaaagaat ctacttctca ccatcggact cgagcccggg
    10381 ccagagcgga ggtatctcct cctccgggaa ctcggcaagc tttttgccca ccgatgcgcg
    10441 cagaaaatcg tccacccagc gaaagacgtc gtgccgtgca atcgatttcc ggagaaggcg
    10501 catccgccgt ttcttttcat gtgcatccat cgcaaatcct tcggcgatcg ccgccgcgac
    10561 ggcgtcgaca tcatacggat tcacaagcag ggcccccttg gaaagctgga ttgccgcgcc
    10621 ggcgaactcg ctcagtacga ggacgccgct tccgtctccc ttgcaggcgc aatactcctt
    10681 gcacacgaga ttcatcccgt ctttcagaga ggttacaagg gccatctccg cgccgcgata
    10741 gagcgcggca agttccgaac gcggaacgga gccgtagcgc caatgcaccg gaacccaccg
    10801 catcgttgaa tactctccgt tgatcctgct gatgagcagc tcgatctcct gcttcaggtc
    10861 aagatactca cccactgact ccctgctcgg gacgacgacc tgcatcaggc tgagtttttc
    10921 acgaagctcg ggataacgct ccagacagcg ctgaaaaccg cgcagccgtt cgggaattcc
    10981 cttcgagtag tcaagacggt cgacgccaag aatgtatttt tgggcgaaca tctcccgctg
    11041 gatgtccttc gagcgggaga tcacctcttt cgaggaggca agggaggcga acgaccggta
    11101 atcgatgctg atcggcaggg cgcctaccag gaaattggat tctccgatgc ggacgcgcac
    11161 cacgtttccc cttccggaaa cctgcatctc cgggaagaag accttcaggc agtcaaggaa
    11221 gttcctcctg tcccgcatcg tctggaatcc gacgaatgcg tagtgaatca gctgcgatag
    11281 aatctctttt ctctgcggca tcttcatgaa gatgtccacg ctggggaacg ggatgtgtaa
    11341 aaagaatgaa gccttgagcg agggtttctt ctcgcgcagc agctttccga gcgggatcag
    11401 gtggtagtcg ttgacccaga tgaaatcatg ttcctcggta tgtttcatca gcaccttcgc
    11461 gaattttcgg ttcgctgcga ggtatccgtc ccagtaggag ggattgaagt cgcacctgct
    11521 ctgaaggtcg tgaaagagcg gccagagcac ggaattcgag aatccggaat agaaggaatc
    11581 cacgtcctcc ttggtcagca tcaccggaag aaggcgataa ccgcttgtct cggaaacggg
    11641 gccgagaatt tcgcggagcg ttgaaagaga aacattctcg tgcgttcctg cccacccgat
    11701 ccaaatcccc cccttgttct tcagcacagg gc
//
LOCUS       DAOPKU010000020        11492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_5902_length_11492_cov_4.617120, whole genome shotgun sequence.
ACCESSION   DAOPKU010000020 DAOPKU010000000
VERSION     DAOPKU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5902_length_11492_cov_4.617120"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PK849_01240"
     CDS             <1..322
                     /locus_tag="PK849_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HPE64776.1"
                     /translation="ITSIIRNMTGMGTLFAFPGSIPGAIAAGLAFKFFSRPVSALAEP
                     LGTGPVGAALSALLLGPAMGKPVGFLTLQTAFLISSIPGALLGMGILHIFQKAPFSRV
                     LRGR"
     gene            482..1459
                     /locus_tag="PK849_01245"
     CDS             482..1459
                     /locus_tag="PK849_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64777.1"
                     /translation="MNPVIQLNVLKCILSIRRTKTEKTNIFFYEILSPAGLFASGIAF
                     SPDDDEKSKKINVEVGAEAFLSFTEELSTRQEAFGKLLSPSVLSSLERLLGEILTAEA
                     VREALENPRDNMKTLEECLREEISSRLGYRATAYLKADRRGEEALAAFLKKDGAVLSS
                     EDANKESAPENISFLVPCAPYIDPVKGTPVASLQKGDFVLVRLPEDEAAPIREKLQRA
                     DPDFDGTVRGEVISVHKDRHGNFTLLVRLSDEFNGVTTLEGNSRIRTAPLAQPATVLP
                     GTAAGSKEKEETPDLPQPSPGDTPGWVYFFVFGAMATLVVILFFIRNFL"
     gene            complement(1440..2090)
                     /gene="thiE"
                     /locus_tag="PK849_01250"
     CDS             complement(1440..2090)
                     /gene="thiE"
                     /locus_tag="PK849_01250"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPE64778.1"
                     /translation="MNTTERIQRALKVYVIPDSTAGAPRSLEEQARLAILGGATMIQL
                     RDKKMTGRELYETAKRLLSLCRPRDIPLIVNDRLDVALASGADGVHLGAEDLPVHAAK
                     RFVPEGFIVGATAHSVEEGIRAEAEGADYVGVGAAFPSGTKKGAAVLGVAGIREVCSA
                     ISLPTVAIGGITAQNAREVLDAGVDGVSVVASVVGQEDIQAAASLLAKAVIRGSYE"
     gene            complement(2087..2914)
                     /gene="thiM"
                     /locus_tag="PK849_01255"
     CDS             complement(2087..2914)
                     /gene="thiM"
                     /locus_tag="PK849_01255"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HPE64779.1"
                     /translation="MAPVLKIVPEDWAEALSVCRARRPLIYAVTNYIAASFQANAILA
                     VGASPVMSPCIHEAEELARSAEGVLINTGAPSEDRELLIRNALTAAEVKRSATLLDPV
                     GYGAASFRRQLVDALLRDFRFAVVKGNRGEISLLAGEKGDVKGVDSEGSYASIVRSVR
                     TVAVKTRSLVCATGETDVLSDGDAVLSVRGGSALLGRITGGGCVLGALMLTLSTAASS
                     ISAGVVCASVLLRLAAERAEKISNGPGTFQAHLLDALHKTHPEELLNEAWRVEEVTE"
     gene            complement(2899..3711)
                     /gene="thiD"
                     /locus_tag="PK849_01260"
     CDS             complement(2899..3711)
                     /gene="thiD"
                     /locus_tag="PK849_01260"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HPE64780.1"
                     /translation="MSVYRGIAVSIAGSDSGGGAGIQADLKTCAALKVFGMTVITALT
                     AQNSTAVTDVWNVPANMVRAQMDALWSDFPVAAAKTGMLSSPEIIRTVVQGVRQWSVT
                     NLVVDPVMVAQSGASLIADDAVEALREELLPHALLVTPNIPEAERLAGFSIRSTEEME
                     RASRRIAELGPKAVLVKGGHLLREDSVTDVFFYKGELTLFEDRRIFTENTHGTGCTLS
                     AAITAELAAGIPLEEAVRRGRQYLRMGLEASFRPGKGWGPLGHAVTPPWLRS"
     regulatory      complement(3775..3878)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3903..4583)
                     /gene="pssA"
                     /locus_tag="PK849_01265"
     CDS             complement(3903..4583)
                     /gene="pssA"
                     /locus_tag="PK849_01265"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPE64781.1"
                     /translation="MVTSGNLLCGTMSLILVLHGRVVPAAWLVFMAVFFDFMDGKVAR
                     RLGGGTRFGLEFDSLADVVSFGVAPAVLFYSVYAERLGVMGALAVSFFALCGALRLAR
                     FNVTHVPGPFQGLPIPAGGLFLASFVLAGAHLSPWLAALLVLGTGALMVSSVPYSSMK
                     KLRKGYARKKRLLLVGGAAISGAILLENAAPLAGISLYVVSGILRFDWEKWLSVPGAE
                     EERVEKSL"
     gene            complement(4633..5253)
                     /locus_tag="PK849_01270"
     CDS             complement(4633..5253)
                     /locus_tag="PK849_01270"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HPE64782.1"
                     /translation="MKLAREGYPLIAFTVFMIIGSVFFAPPVALFLLPVLGLVVWFFR
                     DPERFPDGEGFLSPADGRIVEIEQADLPFTGKAWKIGIFMNPLSVHVNRFPCAGTVEH
                     LEYVPGKKWMAFAPKASEVNERMLVGISTEYGPVLLVQIAGFLARRIVCRLRKGDRLE
                     RGQRYGMIKMGSKVDVYLPAKTRLAVAKGQQVFAGRTVLGGVSCGE"
     gene            complement(5250..7727)
                     /gene="gyrA"
                     /locus_tag="PK849_01275"
     CDS             complement(5250..7727)
                     /gene="gyrA"
                     /locus_tag="PK849_01275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPE64783.1"
                     /translation="MDEQKSEMPIGKVLPLPLVEEIKHSYLDYAMSVIVGRALPDVRD
                     GLKPVQRRILYAMMELGLRSGSSYKKSARVVGETMGKYHPHGDAAIYETMVRMAQDFS
                     MRYPLVDGQGNFGSIDGDPAAAMRYTEAKLFETGELMLADIDEDTVEWGPNFDESLKE
                     PLTLPSMIPNLLVNGSSGIAVGMATNIPPHNLCEVVDALEYLIEADGNIEFGEIYARL
                     PGPDFPTGGVILGRNGIIDAYRTGRGKIILRGKTHVEEGKKGKTSIVITEIPFMVNKT
                     LLIETIASCVQEKHIDGISDIRDESDRDGLRVVLELQRDADAELVLRQLYRRTQLQST
                     FGVINLALVENSPKELPITDMLSLFLEHRRDVVRRRTQFRLDKALARAHIVEGLVKAL
                     DIIDQVIATIRAAQTTQEAREGLVDTLGFSELQAQAILEMRLQRLTGLEREKLDAELA
                     ALLADIERFRSILGNAKILDGVIKDELSLLRKKFGSPRRTEIIDSYEEVSIEDLIPEE
                     DIVVILSKDGYLRRKSLDEYVPQARGGKGRKGAALQDEDEIAVFAVTNTHKDIYLFTS
                     SGRVLAIRGHMIPESRSGKGKLVGKLVPLEDGETVVSMYGRSLEGKSYLFFATKNGIA
                     KRLQLSEILRINRGGKRVLTLDEGDEIAQVRLTSGDDELLFMTAAGQALRVPEQEFRA
                     MGRAARGVKGIRLAKDDSVISCEVVNKERHALVISEKGVAKRTSFEEFTLHHRGGSGV
                     KAMNLGRKTGMLVGSWSVAEEDEIMAITARGRIIRVAVSEIPVLSRTAMGSITVRLDG
                     GDSVADVSVVCGEVCVAAEIPYEEETE"
     gene            complement(7864..8391)
                     /locus_tag="PK849_01280"
     CDS             complement(7864..8391)
                     /locus_tag="PK849_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPE64784.1"
                     /translation="MTLRDNSVIRAILSRRSIRKFRSDAVSPEMRDLLLECAFAAPSA
                     HNRRPCHFIVLEDRALLDAVAEVHDSGKMLFQAPLAIAVCAETASYPEGDLAWVEDCS
                     AALENILVAAKGLGLDGVWLKVMGRHPRNELVRPVLGVPESVQIVGIAALGFGAEEKS
                     PCEGIKEDRVHKNRW"
     gene            8483..9001
                     /locus_tag="PK849_01285"
     CDS             8483..9001
                     /locus_tag="PK849_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HPE64785.1"
                     /translation="MSKKIAVLTEETIHDLEFWYPYYRLAEEGYDLVIVGPEAGKSYT
                     GKMGGVIRSEKSPSEISAKDFAGVVVPGGWGPDKLRTHKAIVDFVRDVHANGGVVAAI
                     CHGGSVLVSARILEGVRATSYKSIKDDMILAGAEWVDEAVVVSKNIVTSRTPSDLAHF
                     GKALVETLKKIK"
     gene            complement(8994..9635)
                     /locus_tag="PK849_01290"
     CDS             complement(8994..9635)
                     /locus_tag="PK849_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPE64786.1"
                     /translation="MGERENTEARILDAVGTILSRKGFRYLGVNAIAREACVDKVLIY
                     RYFGGLQPLLRTFFTRRKYWPTTSEILETAGEGEADPATRAYAILRGYLRELKKSDAA
                     REVLRGEIAGREDVASETAEERYRQGMELLDLLREPDKETEGLTEMAALLSAGFTFLL
                     LRKDVSSRYLGLDLRNEETWERLEKAIHLLVKLYYKEKAPPEGRGLAGQEETT"
     gene            complement(9701..11362)
                     /gene="ilvD"
                     /locus_tag="PK849_01295"
     CDS             complement(9701..11362)
                     /gene="ilvD"
                     /locus_tag="PK849_01295"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPE64787.1"
                     /translation="MRSDNAKKGAERAPHRSLLKASGYTDWEIARPWVGVVNPYNALI
                     PGHIHLNRIAEAVKSAVYAFGGFPLEFPVIGVCDGIAMNHEGMKFSLPSRELIMDSIE
                     VMVRAHALDALVLVTNCDKIIPGMAMAALSLNIPTIMISGGPMLAGTTGGKDVDLSNI
                     FEAVGKRAAGLISDEELREIEDITCPTCGSCAGMFTANTMNCMIEALGFALPGNGTIP
                     AVYSARDRLAKEAGRQIMTLVERNIKPRDILTEKAFDNAVAVDMALGGSTNTALHLPA
                     LAHAAGLSLTLDRFDEISARTPHLCSMSPGGPHHIQDLYAAGGVQAVMARLVQGGLID
                     GNSLTVTGKTVAENLEGVVVKDDAVIRPLDRPYHAQGGLAVLKGNIAPEGSVVKQSAV
                     DPSMLKHSGPARVFDSEEAASKTILEKGIKDGDVVVIRYEGPKGGPGMREMLTPTSSL
                     AGMGMDKTVALITDGRFSGASRGASIGHVSPEAAAGGMIGLVKEGDIIEIDIPARKLH
                     LAVPEDEIARRRSEWKPVETQVDSLFLNRYREFVTSGSKGAVFSD"
BASE COUNT         2440 a         3578 c         3075 g         2399 t
ORIGIN      
        1 tatcaccagc atcatccgaa atatgacggg catggggacg ctgttcgcgt ttcccggaag
       61 catcccgggc gcgatcgctg cgggtttggc tttcaagttt ttctcccggc ctgtcagcgc
      121 gcttgccgaa ccgctcggta ccggtcctgt tggagctgcg ctttcggcgc ttctgcttgg
      181 tcccgccatg ggaaagcctg tcggctttct tacgttacag actgcatttc ttatcagcag
      241 catacccggg gcgcttctcg gaatgggtat tcttcacatc tttcagaagg cgcctttcag
      301 cagggtactc cgagggcggt aagaaaacta cggagcgagt ccttccgttc ttctcaggga
      361 cgaacccgct ccgtagtttt cgaatgcata ttccagccta tttggcgtcc ggggctttcg
      421 cgaacagaga aaacaacgta taatgccatt ctcggaatca ataccgaaag ggagaaaacc
      481 tgtgaatcct gtaatacagt tgaacgtttt gaagtgcatc ctctcaatac ggaggacaaa
      541 aacggaaaag acaaacatct ttttctacga aattttatcc cctgcgggac tgttcgcctc
      601 cggtatagcg ttttcaccgg acgatgacga aaagagtaaa aaaattaacg tcgaagtggg
      661 agccgaggca tttctcagct tcaccgaaga gctctccact cgtcaagaag ctttcgggaa
      721 gctgctctct ccctctgtcc tctcctctct ggagcgcctc ttaggcgaaa tactcaccgc
      781 agaggcagta agagaggcgt tggaaaatcc gcgcgataat atgaaaaccc tggaagagtg
      841 cctccgggaa gagatttctt cccggctcgg gtatcgagca actgcgtatc ttaaggcgga
      901 tcgcagggga gaggaggcgc tcgctgcgtt ccttaaaaaa gacggcgccg ttctttcctc
      961 cgaagatgca aacaaagaat cggcgcccga gaatatctca tttctcgttc cttgcgctcc
     1021 ctatattgat ccggtaaagg ggactccggt agcctcgtta caaaaagggg atttcgtgct
     1081 ggtacgtctc cccgaagatg aagcggctcc catccgggag aagttacagc gtgccgaccc
     1141 cgatttcgac ggtacagtac gcggagaagt catatcggtc cacaaagacc ggcacggcaa
     1201 cttcactctg ctggtccgac tctcggacga attcaacggg gtaacaacct tggaaggcaa
     1261 cagccgcata cgcaccgctc cgctcgcgca gcccgcaacc gttcttccgg gaacggccgc
     1321 aggctcgaaa gaaaaagagg aaacgccaga tcttcctcag ccttctcccg gcgatactcc
     1381 gggttgggtg tattttttcg tgttcggcgc tatggctacc cttgtcgtta tcctcttttt
     1441 tattcgtaac ttcctctgat aacagccttc gcgaggagag acgcggctgc ttgtatatct
     1501 tcctgtccga ctaccgaagc gacgaccgaa accccatcca caccggcatc taaaacttca
     1561 cgggcattct gggctgtgat accgcctatg gcaaccgtcg gaagggaaat cgcggaacaa
     1621 acctcacgta tcccggcaac gccgagaaca gcggctcctt ttttcgttcc tgaaggaaat
     1681 gccgctccta cgccgacata atcggcgcct tcggcttccg cccgtatccc ttcctccaca
     1741 gagtgcgccg tcgcccctac aatgaatcct tcgggaacaa agcgtttcgc cgcatgcacc
     1801 ggaagatcct cggctcccag atgcaccccg tccgctcctg aggcgagggc aacatccaaa
     1861 cggtcgttga cgattaacgg aatatcgcgc ggacggcaca atgaaagaag acgcttcgcc
     1921 gtttcataga gttcgcgtcc cgtcatcttc ttgtcccgca gctgtatcat cgtcgcgccg
     1981 cccagaatcg ccagccgggc ctgctcttcg agagagcgag gcgctcccgc agtgctgtcg
     2041 ggaataacgt acaccttcaa cgctctttgt attcgttccg ttgtgttcat tccgttacct
     2101 cctccactct ccacgcttcg ttgagcaact cctccggatg cgtcttgtgc aaggcatcca
     2161 acaagtgcgc ctgaaaggtt ccgggaccgt tcgagatctt ttccgcgcgt tccgccgcca
     2221 gccgaaggag aacggaagca cagacaaccc cggcggagat actggaagcc gccgtactca
     2281 gggtaagcat caacgcgccc aacacgcacc ctccgcctgt gatccgtccg agcaacgcgc
     2341 tgcctccccg caccgacaac acggcatcgc cgtcgctcag cacatccgtc tcgccggtag
     2401 cgcagacgag cgaacgcgtc tttactgcaa ctgttctgac ggaacggaca atggacgcgt
     2461 agctcccctc tgaatccacc cccttcacgt cacccttctc tcccgccaaa agagagatct
     2521 cgccccggtt ccccttgacg actgcaaaac gaaaatctcg gagaagcgcg tcgacaagct
     2581 gtctgcgaaa tgaagcagcg ccgtatccta cgggatcaag cagcgtagcg cttcttttta
     2641 cctctgcggc ggtaagcgcg ttccggataa gcagttctct atcctccgag ggagcgcctg
     2701 tattgattag cactccttcc gcgctcctcg caagctcttc cgcttcatga atacagggcg
     2761 acataacagg agaagcccca accgcgagga tcgcgttcgc ttggaaagac gcggcgatat
     2821 aattcgtgac ggcgtaaatc aggggacggc gagcacggca gacactcagc gcctccgccc
     2881 agtcttcggg gactattttc aagaccggag ccatggcgga gtcaccgcat gccccagagg
     2941 tccccaccct tttccggggc gaaaactcgc ctccagcccc attcgcagat actgccttcc
     3001 ccggcgaaca gcttcctcca aggggatacc ggccgcaagc tcggcggtaa tcgctgcgct
     3061 cagcgtgcat cccgtgccgt gcgtattttc cgtgaaaatc ctccggtctt caaagagcgt
     3121 gagctcccct ttataaaaga aaacatccgt tacggaatcc tcgcggagaa gatgcccgcc
     3181 ctttaccagc acggctttcg ggcctagctc cgctattctc ctcgacgcgc gttccatctc
     3241 ctccgtggaa cggatcgaaa aaccggccag acgttcagcc tcgggaatat tcggggtcac
     3301 aaggagcgca tgaggaagca attcctctct gagcgcttcc acggcgtcat ccgcgatgag
     3361 cgacgcgccg ctctgtgcga ccatgacggg atcgaccacg agattcgtca cactccattg
     3421 tcgcacaccc tgcaccaccg tccgtatgat ctcgggcgag ctcagcatcc ccgtctttgc
     3481 ggcggcgacc ggaaagtccg accagagggc gtccatctgc gcgcggacca tatttgcagg
     3541 gacattccat acatccgtga cggcagtgct gttctgcgct gtcaaggctg tgatcaccgt
     3601 catgccgaac accttcaaag cggcgcacgt cttcagatcg gcttgtattc ccgcgccccc
     3661 tcctgaatcg cttcccgcga tggacaccgc tattccccga tacacactca tcacacattc
     3721 cgcctttcct caaataaaaa aggcccgttc gcaatgaacg gaccttctcc aggctcgctg
     3781 aaaagagccg ctccctacgc cggcattatc cggatcaggt agagggtcga aggcctccgc
     3841 cttcctctca gcccgttcgg gctcccccgt cactatcgat tgtaaaaaaa gaaaaaagac
     3901 actcaaaggc tcttttcgac ccgctcctcc tctgctccgg gaacggaaag ccacttttcc
     3961 cagtcaaaac gcagtatacc gctgacgaca taaagcgaaa ttccggcaag aggcgccgcg
     4021 ttctccagca ggattgctcc gctgatcgcg gcgcccccca ccaggagaag ccgcttcttt
     4081 cgcgcgtacc ccttgcgcag cttcttcatg ctgctgtagg gtacgctcga caccatcagc
     4141 gcgcctgttc cgagcacaag caacgccgcg agccatggag agagatgagc tccggcgaga
     4201 acaaaggagg ccagaaaaag cccgcctgct ggaataggca gcccctggaa gggcccgggc
     4261 acgtgtgtga cgttgaatcg cgcgagacgc agggcgccgc aaagcgcaaa gaaggagacg
     4321 gcaagcgcgc ccatgacgcc gagccgctcc gcatacaccg agtaaaaaag aacggccgga
     4381 gcaacgccga agctcacaac gtctgccagg ctgtcgaatt caagaccgaa gcgcgtcccg
     4441 ccgccgagcc gcctcgcaac ctttccgtcc atgaagtcga agaaaaccgc catgaaaacg
     4501 agccacgccg cagggaccac cctgccgtgg agcacaagaa tgagcgacat cgtcccgcag
     4561 agcagattgc cgctcgtgac catgttgggc gctatctcgc ggaaagggag aggacgccgt
     4621 ttctttctga tgctactcgc cacaggacac acctcccaga accgtacgtc ctgcaaaaac
     4681 ctgctgcccc tttgcaacgg caaggcgagt cttcgcaggc aaatagacat cgactttcga
     4741 ccccatcttg atcatcccgt accgctgccc tctttcgagc cggtctccct tgcggagacg
     4801 gcagacgatg cggcgcgcca gaaacccggc gatctgcacc aggaggacgg gaccgtactc
     4861 cgtggaaata ccgacgagca tccgctcgtt gacttccgaa gccttggggg cgaacgccat
     4921 ccatttcttc cccgggacgt actcaagatg ctccacagta ccggcgcagg ggaaacggtt
     4981 gacgtgcacg ctcagcgggt tcataaaaat accgatcttc cacgcctttc ctgtgaaggg
     5041 aaggtccgcc tgctcgattt ccactatacg cccgtccgcg ggagagagaa atccctcgcc
     5101 gtccgggaat cgttcagggt cgcgaaaaaa ccagacgacg aggccaagga cagggagaag
     5161 aaagagcgcc acgggagggg cgaagaaaac gctcccgata atcatgaaca ctgtgaatgc
     5221 gataagcggg tatccttctc gagccagttt cattcagtct cctcctcgta tgggatctcc
     5281 gcggcaacgc aaacctctcc gcagacaacg ctcacgtccg ctacgctgtc ccccccgtcg
     5341 aggcgaacag tgattgaacc catagccgtt ctgcttaaaa cagggatctc cgaaacggcg
     5401 acgcggataa ttctgcctcg cgccgtgatc gccataattt cgtcttcttc cgccacagac
     5461 cagctgccca caagcatccc ggtctttctt ccgaggttca tcgccttcac gccggatccg
     5521 cccctgtgat ggagggtaaa ctcctcaaag ctcgtcctct tggctacccc cttttcactg
     5581 atgacaagcg catgccgctc tttattcacg acttcacaac ttattacgga gtcgtccttt
     5641 gcaaggcgta tgcccttgac gccgcgcgcg gccctcccca ttgcgcggaa ctcttgttcg
     5701 ggaacgcgga gagcctgtcc agcagcggtc ataaagagca gttcgtcatc cccggacgtc
     5761 aggcgcacct gcgcgatttc gtcgccttca tcaagggtaa gcactcgttt ccctcccctg
     5821 ttaatgcgca aaatctccga aagttgcaga cgtttcgcta tgccgttttt cgtcgcgaag
     5881 aagagatagc tcttcccctc caagctccgt ccgtacatgg agacaaccgt ctccccgtcc
     5941 tccagcggca caagcttccc gacgagtttg cccttgccgg agcgtgattc cggaatcatg
     6001 tgcccccgta tcgcgagcac acgcccggag gatgtaaaca aataaatgtc cttgtgggtg
     6061 ttcgtcaccg caaaaacggc gatctcatcc tcgtcctgga gcgcggcgcc tttccggccc
     6121 tttccgcccc tcgcctgcgg gacgtactcg tcgaggctct tccggcggag atagccgtcc
     6181 ttggagagaa tcacgacgat atcttcctcc gggatgagat cttctatgga cacctcctcg
     6241 tagctgtcga ttatctccgt cctcctgggg ctgccgaact tctttctcag aagactcagt
     6301 tcgtctttga tcacgccgtc cagaattttc gcattgccaa gaatgctccg gaagcgttcg
     6361 atatcggcga gaagcgcggc cagctcagcg tcgagctttt ccctttcaag gcctgtgagg
     6421 cgctgcaggc gcatttcgag tatcgcctgc gcctgaagct ccgaaaatcc gagggtgtcg
     6481 accagccctt cccttgcttc ctgcgtcgtc tgcgccgccc gaatggtcgc gatgacctgg
     6541 tcgatgatat caagcgcttt gacgagccct tcgacgatat gcgcacgggc gagggccttg
     6601 tcgagacgga actgcgtccg tctgcggacg acgtcgcggc ggtgttcgag gaagagagag
     6661 agcatgtcgg tgatggggag ctctttcgga ctgttctcca ccagcgcgag attgatgact
     6721 ccgaatgtcg actggagctg tgtgcggcgg taaagctggc ggagcacaag ttccgcgtcg
     6781 gcgtcgcgct gcagttcgag aacgacgcga agtccgtcac ggtccgactc gtcccggatg
     6841 tcggaaatgc cgtctatgtg cttttcctgc acgcaggagg cgatcgtctc gatcaacagc
     6901 gttttgttca ccataaaggg gatttcggtg atgacgatcg aggtcttgcc tttcttcccc
     6961 tcctccacat gcgttttgcc gcggaggatg atctttcccc gcccggtgcg gtacgcgtcg
     7021 ataatgccat tgcgtccgag aatcactccg ccggtcggga aatcagggcc gggcagccgc
     7081 gcgtaaatct cgccgaactc aatatttccg tcggcttcaa tcaggtattc gagtgcatcc
     7141 acaacttcgc acaggttatg cgggggaatg ttcgtcgcca tgcccacagc gatcccggaa
     7201 cttccgttca ccaggagatt cgggatcatc gagggcaggg tcaacggctc cttgagcgat
     7261 tcgtcgaagt tcggccccca ttccacggtg tcttcgtcga tatccgccag catcagctct
     7321 cctgtttcga agagtttggc ctcggtgtat cgcattgccg ccgcaggatc gccgtcgatg
     7381 gagccgaagt tgccctgtcc gtcgaccagc ggatagcgca tgctgaagtc ctgcgccatt
     7441 cggaccatgg tttcgtagat cgccgcgtcg ccgtgcgggt ggtacttacc catcgtctct
     7501 cccacgacgc gcgccgactt tttatacgag gatcctgacc ggagccccag ctccatcatt
     7561 gcgtacagaa tccgccgctg caccggtttc agaccgtccc gcacatcggg gagcgcgcgc
     7621 cccacgatga cgctcatcgc gtaatcgaga tagctgtgtt tgatctcctc taccagagga
     7681 agagggagca ccttgccaat gggcatttca cttttttgtt cgtccatacg atccttcaac
     7741 ctccaaagct gattcgccgt gcaaaaagcc ggcgacgtgt acagcataac ctgtatattg
     7801 tatcacgtca ggaggagtag tgagctgtcc caacacgcat gaatgctctt cccgggtgtt
     7861 tatttaccaa cggtttttgt gcacgcggtc ttctttgatt ccttcgcagg gacttttttc
     7921 ctccgcaccg aatccgagcg cggcgatacc cacaatctgc accgattccg gtacgccgag
     7981 aacaggacgc accagttcgt tccgcgggtg acgccccatc actttcagcc agacgccgtc
     8041 gaggccaagc ccctttgcgg cgacaaggat gttctcaaga gccgcagagc agtcctccac
     8101 ccaggcaaga tctccttcag gataggatgc ggtttcggcg cagacggcga tcgccagggg
     8161 cgcctgaaaa agcattttcc ccgagtcgtg gacttccgcc accgcatcga gcaaagcccg
     8221 atcctcgaga acaataaagt ggcacggacg ccggttgtgc gccgacggcg cggcaaatgc
     8281 gcactccaga agcaggtcgc gcatctccgg cgatacagca tcggagcgaa acttcctgat
     8341 actcctccgg gagagaatcg ccctaatcac agaattatcc ctcaatgtca ttgtcatcgc
     8401 ctcctgtaaa ggatgtttta tgatagacta taaacgtgaa gagtttcaac aatcgtatca
     8461 ccaaaagaag gagggacaga gtatgtccaa gaaaatagcc gtacttacag aggagaccat
     8521 tcacgacctc gaattctggt acccctacta ccgtcttgcg gaagaggggt acgatctcgt
     8581 catcgtcggc ccggaagcgg gaaagagcta taccgggaag atgggcggcg tgatccgctc
     8641 ggagaaatca ccttcggaga tctccgcgaa ggacttcgca ggcgtcgtcg tccccggagg
     8701 gtggggcccc gataagctca gaacacacaa ggcaatcgtt gatttcgtca gggacgtcca
     8761 tgccaacggc ggcgtcgtcg cggcaatatg ccacggcggc agcgtccttg tatcggcgcg
     8821 tattctggaa ggcgttcgcg ccacttccta caagagcatc aaagacgata tgattctcgc
     8881 cggagcggaa tgggttgacg aggcggtagt cgtctcgaag aatatcgtca caagccgcac
     8941 gccttccgat ctggcgcact tcggcaaggc gctcgtcgaa acgctcaaaa agatcaagta
     9001 gtttcttcct gtcctgcaag gcctcgcccc tccggcgggg ccttttcctt gtaatacaac
     9061 ttcacaagaa ggtgtatggc tttctccagt cgctcccacg tctcctcgtt ccgcaggtcg
     9121 agcccgagat accgggagga tacatccttt cgcagcagca agaaagtgaa tcccgcagag
     9181 aggagcgcgg ccatctcggt cagtccttcc gtctccttat cgggttcacg aagaaggtcg
     9241 agcagctcca tcccctgccg gtaacgttct tccgccgttt ccgaagcgac gtcttctctt
     9301 cctgcaattt ctccccgcag aacttcacgg gcagcatccg actttttcag ctcccggaga
     9361 taccccctga ggatcgcgta cgccctcgtc gccggatcgg cctcgccttc accggcggtc
     9421 tccaggattt ccgaggttgt cggccagtac ttcctccgcg tgaaaaacgt ccgcagaagc
     9481 ggttgaagcc ctccaaaata acgatagatg agcaccttgt ccacacaagc ttcccgggca
     9541 atggcgttca ccccaaggta ccggaaacct tttcttgaaa ggatggtccc taccgcgtcg
     9601 aggatgcggg cttcggtatt ctccctctcc cccacattct tttccccctt tggaaagcga
     9661 gaaggggctt ccggagaaag ccccttcaca gcaaaaatca ttagtcgctg aacaccgctc
     9721 ctttggagcc ggatgtaaca aactcgcgat acctgttgag gaaaagactg tcgacttggg
     9781 tctctacagg tttccattcc gatctgcggc gggcgatctc gtcttcagga acggcgaggt
     9841 gcagtttccg tgcggggatg tcgatctcga tgatgtctcc ttctttcacg aggccgatca
     9901 ttccgcccgc agccgcctcc ggagagacat gaccgataga tgcgccgcgt gaagcgccgg
     9961 agaaccgtcc gtcggtgatg agcgcaacgg tcttatccat ccccattccg gcgagcgaag
    10021 aggtcggagt aagcatctcg cgcatccccg gtccgccctt ggggccttcg taacggatga
    10081 caaccacgtc gccgtccttg atccccttct caagaatggt cttggacgcc gcttcctcgc
    10141 tgtcgaagac gcgggccgga ccggagtgct tcaacattga aggatcaacg gcggactgct
    10201 tgacaacgct cccctccgga gcgatgtttc ccttaagcac ggcaagaccg ccctgggcgt
    10261 ggtacggcct gtcgagcgga cggataacag cgtcgtcctt tacaacgacc ccctcgaggt
    10321 tttcggctac ggtcttccct gttactgtaa gcgagttccc gtcgataaga ccgccctgca
    10381 caagacgcgc catcacggcc tggactccgc ctgcggcgta gaggtcctgg atatggtgcg
    10441 gcccgccggg gctcatgctg cacagatgcg gcgtacgagc gctgatctcg tcgaacctgt
    10501 cgagcgtcag cgaaagacct gcggcatgcg ccaatgccgg aagatggagc gcagtgttgg
    10561 tagagccgcc gagcgccata tcgacggcaa cggcgttgtc gaaggccttc tccgtgagga
    10621 tgtctctggg cttgatgttc ctctcgacca gcgtcatgat ttgccgtccg gcctccttgg
    10681 ccagtcggtc ccgggccgag tagacggcag gaatggttcc gttcccgggg agagcaaaac
    10741 cgagcgcctc aatcatgcag ttcatggtat tcgcggtgaa cataccggcg cacgaaccgc
    10801 atgtcggaca ggtgatatcc tctatctcgc ggagctcctc gtcggaaatc aacccggcgg
    10861 ccctcttgcc gacagcctcg aagatgttgc tgagatcgac gtccttgcct cccgtcgttc
    10921 cggcgagcat cggcccgccg ctgatcatga tcgtcgggat gttcagcgag agcgccgcca
    10981 tcgccatgcc ggggataatc ttgtcgcagt tggtgacgag aacgagagcg tcaagcgcat
    11041 gcgcccgcac catcacctcg atggaatcca tgatcagctc gcggctgggg agcgagaact
    11101 tcatcccctc gtgattcatg gcgatgccgt cgcagacgcc gatgacgggg aattccagag
    11161 ggaagccccc gaatgcgtac acagcggatt tcactgcctc cgcgatccgg ttcagatgaa
    11221 tatgaccggg aataagcgcg ttataggggt tgaccacgcc cacccaggga cgggcgatct
    11281 cccaatcggt atatccggac gctttcaaca gcgagcggtg aggcgcccgt tccgcgccct
    11341 tctttgcgtt gtcgcttcgc ataacaatta cctcctcttg aaattacttt tgaagcgtta
    11401 tattcctccc ggtccccaga cgagcattgg aagagagagg atgagcgatg gcacataggt
    11461 cactaaaaga agaatagcga tgaggacaaa aa
//
LOCUS       DAOPKU010000021        11445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_5927_length_11445_cov_4.311150, whole genome shotgun sequence.
ACCESSION   DAOPKU010000021 DAOPKU010000000
VERSION     DAOPKU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11445
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5927_length_11445_cov_4.311150"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PK849_01300"
     CDS             <1..973
                     /locus_tag="PK849_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64788.1"
                     /translation="VSVMGLSFAEAEFGGLQARAIADAVNRLAHGIRSELGGESSHVL
                     NVSGNEVVIDVGSTMGAKEGQLYLVYAEGKTILGMSGESLGRDKMPLAVVKVRDVASG
                     HSTCAVASNCSLSLVRRGDKIEPVTAAKAKTMKFVSSRPAASSETFNQIFGPQGQSGA
                     VASVPQDAPAPAAETPAKPAVHEDKKEEPAPAAVSSVASAPRSSAPVAQAGEAKKIPG
                     FDPNTSTDVKVIQTYNLDMGTSNMLRIAHVNAMNSLNSRKVKVAYEKFVEAAGKYDGH
                     YLAAYWAGVAAHRLKKADDSLQWMNRALAINPQYQPAADYKSKKLKQ"
     gene            1068..1544
                     /locus_tag="PK849_01305"
     CDS             1068..1544
                     /locus_tag="PK849_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64789.1"
                     /translation="MNTRLFHRSNALSISVRVLLVLLFVLAVQTAGIAAETETEVEKE
                     KALIDWGQNYIEGSGMAVAPEGTTGAQAKALARRGAMVDLQRNMLEFVCGVQIDARTV
                     MDDFMAEDRVRSEVHGLIRNIEILKGEWDGESYTVTGRIKTEQLRLIVNYVLTLVQ"
     assembly_gap    1714..1813
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1930..2469)
                     /locus_tag="PK849_01310"
     CDS             complement(1930..2469)
                     /locus_tag="PK849_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64790.1"
                     /translation="MIRLATTVSGEGGCIASYVGGASLMRNGEYEQGRDLALPVLDRC
                     YGASVQFWKDSERLRTLVAGEVSLHAGGQGDYGTIITMEDKLGKDILTDRLLVRDAKG
                     ILEGRTRLGDVLRFHKARAYTTAQMPNEAKQTLKELEFASGKVFVDGEVTGLKDAIAK
                     LKIQVDGLARLIRSFFRAA"
     gene            complement(2709..3875)
                     /locus_tag="PK849_01315"
     CDS             complement(2709..3875)
                     /locus_tag="PK849_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPE64791.1"
                     /translation="MNGRRNISAFSVLMLLTLLALPGRAFAASMIVAVQPFLAQGTSF
                     HLGATVGDIVSTSLAGNRDITLVERSRLAEVARQQRLAMSGVVETATAAKAGKLVGAR
                     YFILGAVSRFGALLVATARLVDVESGRVLASFERTSKQGEDETTLVSRNLASDILAFI
                     SGDAPAPGDPTSDYKYYLYEALGYYNMGEYRKSLPFWEKMTQLSPRNGLLRFIVAGLH
                     FQTRRYSDALLAAQQAVTWEPSFAEAHLLVGKAYFLLGDNHKATPPLDKALELDPRLV
                     EAIFLKGSAFKNRKRVEEAADYFLEAIQIDKTYIPAYLALGQLLLENGAADEASGILL
                     SGLQQQPNNGNLRFLLGAAQIMRGNLQGAKEQLSALKTIDAALAKKLEDLLPKQ"
     gene            complement(3914..4633)
                     /locus_tag="PK849_01320"
     CDS             complement(3914..4633)
                     /locus_tag="PK849_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64792.1"
                     /translation="MNTLKRISLGLSAVLCILLIATALPVFAEVQVFIDAHTVRVYDP
                     VLKRQSILNDFRTSGYRVHNHLALVWSEDAAWVYDVRTQQWLSESDFRTLLGSLSDEY
                     ALVWNENEAAIFDAKKQEWIRSDYIPWKLTGASLSRGMTILTGEEGLVVYDPVLKKWL
                     SSQFFPYGALKKAQAGDVLAAAWSDNDLVLYDMTIHDWVVKEGISPQACIIDGYKASI
                     FTADTVYTYDAVSHRWSSKNR"
     gene            complement(4684..5469)
                     /locus_tag="PK849_01325"
     CDS             complement(4684..5469)
                     /locus_tag="PK849_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64793.1"
                     /translation="MKKMFAVCMAVILFFALLPSGEARAELPKGKTIAVLVKGTSHAP
                     LARSILIRALLDEGYKAVDEKQLERIRQNKAAILALEGNVEAIMALGRTFGFSVLLSG
                     SVAVPSPVRNEFGLFTATATVSATACTASNARQVAAGSASAKEIGYTADEAARKAVEV
                     ATRSAAASLLGKTPPQPPDSAGATFLVIAPARSFAEAHALAASCRDAGASNASVARFA
                     GGRAEIDVSYSGSAGQLLAALLAKRPDLKEETVEGNTLRLVGE"
     gene            complement(5475..5945)
                     /locus_tag="PK849_01330"
     CDS             complement(5475..5945)
                     /locus_tag="PK849_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64794.1"
                     /translation="MRVCRVLVCLLVVFFSSAAFASLPAEGTEDLAALFQSIVESKSV
                     AEDIQVFRFGVVDWKGESVTSQGKAPLPSTSPQDQMLAKRGALTDARRNLLCLLYEIR
                     HGLPEKITSIEIEGEVVDGQVDFQGVKDGVYTVEVTVPLKRFFTESRIVRADVR"
     gene            complement(5949..7088)
                     /locus_tag="PK849_01335"
     CDS             complement(5949..7088)
                     /locus_tag="PK849_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64795.1"
                     /translation="MRRTLLSVVCTCALLLLLLPALASGTEKIVVEAEGMAAIANGDV
                     ARAREEARRHLYRDALEKGIGARVQGITEMKDFEVVRDTVFSRSEGLVTATENLKEEQ
                     TNDGILRLTAQCTVSASTLDGVLGPAVIDALGNPRIMVLIDEVVDGERQFLSTAEGEV
                     LKAFQKAGYMLVDPGQAGAIRKGEIEAAKISGDTAVLQELARSFQSDVLIHGKAQAST
                     ATKQKVSGVVLYAVNSQLQLKAIITQTAHIIGAEGSEQRVQGTSPNDGAMKAFQKLSP
                     RMAGSLLHTVAYALVSGASGSIPGRSFKVTVSDLSFAEARSLRDSLGEVRGVNGVYQR
                     AYANRTLELDVSAETSSEEIAIALEALGVEITGITAGTVNGRKGR"
     gene            complement(7093..8025)
                     /locus_tag="PK849_01340"
     CDS             complement(7093..8025)
                     /locus_tag="PK849_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64796.1"
                     /translation="MSQKRFVSCLLFLLLLGVPAFATEEVFLPPIEDLPEAGDSVPAA
                     PAVDVSTAFDTGTLPAPSPSALIVGTEFARVNWTDDYVEASGQAVAPTGKEKTAQGKL
                     LARRGAIVDLQRNLLEAIQGVRVDAKTTMTDFMANDTVRTEVQGLIKGIEISDASWDG
                     EIYTVTGRVKLEKVRTAVLPGLPKAPGQKPVPPTPPAGKISASGLLIDATHLPLIPAM
                     IFRVVDENGKEVYGIDFADAERFLASGLCDYHTNLAYAKGAPRIASTPLVAKAVRTVA
                     PQNVDIVISNADAAKVRGSSYDFRVPCRVTVVKR"
     gene            complement(8251..9330)
                     /locus_tag="PK849_01345"
     CDS             complement(8251..9330)
                     /locus_tag="PK849_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64797.1"
                     /translation="MKIVIDSITKDFGSFDDPGSIFRAVDAVSLEVRDGELVTLLGPS
                     GCGKTTLLRMLAGFEDPTSGNIFFGERRMNDIAPNKRNATMVFQSYAIFPHLNVRDNI
                     AFGLKLKDLSKKEIRERTERVVELVGLEGLEERQPSQLSGGQQQRVALARAVVMEPSV
                     LLFDEPLSNLDAKLREQMRIDIRKLQQRLGITSIYVTHDQIEAMSISDRVVVMNRGRI
                     EQMGSPRELYARPINRFVASFIGKAAFLPAEKDASGAWTLLGKPWRPEAGEELTAGAY
                     EAMIRPEAVRLSPLEDSGRNAIAGTVLRATYLGSVMEYEVELPGTEPVTVHIPNPFER
                     DPLPPGAEAELRFLPEGLHFLPAEA"
     gene            complement(9335..11026)
                     /locus_tag="PK849_01350"
     CDS             complement(9335..11026)
                     /locus_tag="PK849_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPE64798.1"
                     /translation="MTAFTRGRREIQLLARDPLLALLVVLLFVSLGVFVVFPLLSVLV
                     KSFQSEQGAFSLENYTRFLTFNYLRSSLWNSLTVGFCTAACSVLVGYAAAFTITRTSV
                     PWKKALHLLFILPIISPPFTSALSILMLFGANGLITRGLLGIRNYNIYGFKGVLLSQI
                     FTFAPVAYLTLKGVMESLNPTLEDAAMNVGAGRLQTFLRVTLPLSLPGIASAFLVVLI
                     ESLADFGNPLVLAGSRFPMLSTQAYLEITGSFNLPLGAALAVVLLIPSITSFAIQRYY
                     LQKRQYTTVTGKPVASSSKLVSRNARRGLIAFSAAFCGLVLLFYGTIFVGAFTRVWGF
                     DFTPTLHHFAYAFSVGMGTIRDTLIVAICATPLSGVLGMLIAFLVVRTSFPGKGVMEF
                     TSILNFAVPGTVIGIGYILAFNTPPLMLIGTLAILVLNFVFRYIPVGIQSGIAVLRQI
                     DPSIEEAAQNLGADRLTTFRKVTLPIIAPAFFSGLVFAFVRAMTAISAAIFLVSANWN
                     LLTVQILNQVGSGRLGVAAAFSVILVVIVTAAMAIIARLVPGRAGGMAAIQIQEE"
     gene            complement(11099..>11445)
                     /locus_tag="PK849_01355"
     CDS             complement(11099..>11445)
                     /locus_tag="PK849_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPE64799.1"
                     /translation="QFTPAFLKLVDEGYPAKGVVPAEGVGYEAAALSIIKGAKNLDSA
                     KALVDWIVSKEGQQVLSAKKTYFFPVRTDVSAGEGLPPLSEIKLIDYDRLEAAREKKR
                     LVDRWVTEVLGQ"
BASE COUNT         2187 a         3464 c         3504 g         2190 t
ORIGIN      
        1 tgtctctgtg atggggcttt cgttcgccga agcggagttc ggcggtcttc aggcgcgcgc
       61 gatagcggac gcggtcaatc gtctggctca cggaatccgg tcggaactcg gaggcgagag
      121 ttcccacgtg ctcaacgtgt ccgggaacga ggttgtcatc gatgtcggtt cgacgatggg
      181 cgcaaaggaa gggcagctgt atctggttta tgccgaagga aaaacgattc tcgggatgag
      241 cggagagtct ttgggtagag ataaaatgcc gcttgccgtt gtgaaagtgc gggatgtcgc
      301 gagcggacac agcacctgcg ccgtcgcttc caactgcagt ctgagcctgg tgcggcgcgg
      361 cgacaagatc gagccggtca ctgcggcgaa ggcgaagacg atgaagttcg tctccagcag
      421 gcctgcggcc tcgagcgaga cctttaatca gattttcggc cctcaaggcc aaagcggagc
      481 tgtagcgtcc gttcctcagg acgccccggc gcctgctgcg gagacaccag caaagcctgc
      541 ggtgcatgaa gacaaaaaag aagagcccgc gccggcggca gtatcctccg ttgcgtccgc
      601 tccccggagt tccgcacctg tcgctcaggc gggcgaggcg aagaagattc ccggtttcga
      661 tcccaacacg tcgaccgacg tgaaagtcat tcagacatac aacctggata tgggtacatc
      721 caacatgctc cgcatcgccc atgtcaacgc gatgaactct ctgaacagca gaaaggtgaa
      781 agttgcttac gagaagttcg tcgaggcggc gggcaagtac gacgggcatt atcttgcggc
      841 gtactgggcg ggagtggcgg cgcaccggct gaagaaggcc gacgactccc tccagtggat
      901 gaacagggcg cttgctatca atcctcagta ccaaccggcc gccgactaca agagcaaaaa
      961 actgaaacag tagcaaacta tccgcccccg tcggctcgct cctgcggggg cggcgctgta
     1021 tcccgcgctt gaacgcggga agatcgacat cttaggaggt catgacaatg aatacccgtc
     1081 tctttcatcg ctccaatgct ctttcgatat ccgtacgcgt tctgctcgtg cttctgttcg
     1141 tccttgccgt tcaaacggcc ggaatagcgg cggaaaccga aacggaggtc gagaaggaaa
     1201 aagcgctcat cgactggggc cagaactaca tcgagggctc cggcatggcc gtcgccccgg
     1261 aaggcacaac gggcgcccag gcaaaggccc ttgcccgcag aggcgcgatg gtcgacttgc
     1321 agcgtaacat gctcgagttc gtctgcggcg tccagataga cgcccgcacc gtcatggacg
     1381 acttcatggc ggaagatcgc gtccgctccg aggtccacgg tctgatccgc aacatagaga
     1441 ttctcaaggg cgagtgggac ggcgaaagct acaccgtcac cggccgcatc aaaacagagc
     1501 agctccgcct gatcgtgaac tacgtgctga cgctggtgca gtaacgaaga agggcggaga
     1561 atgcgattgc agctttcccc gcccttcttc tcaaatgcat gtcgttttcc ggatatgatc
     1621 aatcgctcct cacacgtccg ggccgcccag ttccatcgcg atgtcctcgg cgaggaagcc
     1681 ggatgcttcg ataagcccct cctcgccctg gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnggaagag gagcggcgag gatgctcgct cccaagagca acagcagaat
     1861 tagccgacac gcttttttac aggacatcct cgatcccccc tttgcagcaa ggtcgtttcc
     1921 gctccgagat catgccgccc tgaagaacga gcgtatcagg cgagccaatc catcaacttg
     1981 aatcttcaat ttggcgatgg cgtctttcag tccggtgacc tcgccgtcca cgaagacctt
     2041 cccggaggcg aactcaagct ctttgagcgt ctgcttcgct tcgttcggca tctgcgccgt
     2101 ggtgtacgcg cgcgccttgt ggaagcggag aacgtcgccg aggcgggtcc gcccctcgag
     2161 gatgcctttc gcgtcgcgga ccagcagcct gtcggtcagt atgtcctttc cgagtttgtc
     2221 ctccatcgtg ataattgttc cgtagtcgcc ctgtcctccg gcgtgaaggg aaacctcccc
     2281 ggcgaccagt gttctcaggc gttcgctgtc tttccagaat tggaccgacg cgccgtagca
     2341 tctgtccaaa acggggagcg cgagatcgcg tccctgttcg tactcgccgt tccgcatcag
     2401 gctcgctccg ccgacgtagg atgcgataca ccctccttcg ccgcttacgg ttgttgcaag
     2461 tcgaatcatc tggtcaagat tgcgtccgct gagcgttcct tcgttcaaaa aaatctgtac
     2521 cagtttgtcg atttctttct gatctgccgt tgatgctcca tcgccgccgc ctccggaacc
     2581 tccggagccg ccgcctcctc agccgaaccc cgcgacgggc gccagaagca gcgcgccgcc
     2641 aagtacaaaa acgctcagaa gaagtacaaa tttctttctc attaccgcca catcctttct
     2701 gttctcgatc actgtttggg aagcaaatcc tccagtttct tcgcaagcgc cgcatctatt
     2761 gtcttcaggg cggaaagctg ttctttcgct ccctgaaggt ttccccgcat gatctgggca
     2821 gcgcccagaa ggaagcggag attgccgttg ttcggctgct gctggagccc cgaaagcaaa
     2881 attccggagg cttcgtccgc cgcgccgttc tcaagcagaa gctgcccgag ggccagatag
     2941 gccggaatat aggtcttgtc gatctgaatg gcctccagga agtaatccgc tgcttcctcg
     3001 accctctttc tgttcttgaa tgccgacccc ttcagaaaga tcgcctcaac gaggcgggga
     3061 tcgagttcga gcgccttgtc caacggcggc gtggctttgt ggttgtcgcc gagaagaaag
     3121 tacgctttgc cgacgagcag gtgtgcctcc gcgaaggatg gctcccatgt gactgcctgc
     3181 tgggccgcga gcagcgcgtc ggaataacgc ctcgtctgga agtggagccc cgcaacgatg
     3241 aaacgcagaa gtccgtttct cgggctgagc tgcgtcattt tctcccagaa cggaagcgac
     3301 ttgcggtact ctcccatatt atagtacccc aacgcctcgt agagatagta cttgtagtcg
     3361 ctcgtcggat ctccgggcgc gggggcgtcg ccgctgatga aggccagaat gtcggaggcc
     3421 aggtttcgcg atacgagcgt cgtttcgtcc tctccctgtt tcgaggttcg ttcgaagctc
     3481 gcgagcacac ggccgctctc cacgtcgaca agccgcgccg tggccacgag cagggccccg
     3541 aatctgctga ccgcgccgag gatgaagtag cgcgcgccga cgagttttcc cgccttcgcc
     3601 gccgtggcgg tttccacgac gccggacatg gcgagccgct gctgacgggc gacctcggcc
     3661 agtcgggatc gttcaacgag cgtgatgtca cggttccctg cgagcgaagt ggagacgata
     3721 tcgccgaccg tcgcgccgag gtgaaagctc gttccctggg cgaggaaagg ctgcacggcg
     3781 acgatcattg aagccgcgaa agccctcccc ggcagtgcga gcagtgtaag cagcatcagc
     3841 accgagaaag ccgatatgtt tcgcctgccg ttcattgtcg gagcgcctcc tctccgacgt
     3901 ctcgcgccgc tcctcatctg ttcttgctgc tccatctgtg gctgaccgcg tcgtaggtgt
     3961 ataccgtgtc cgcggtgaag atggacgcct tgtacccgtc gataatgcat gcctgaggcg
     4021 aaatcccctc cttgaccacc caatcatgga tcgtcatgtc gtagagcacc aggtcgttgt
     4081 ccgaccaagc ggccgcaagc acgtcgcccg cctgcgcctt tttcagcgcg ccgtaaggga
     4141 agaactggga cgaaagccat tttttcagca ccggatcata caccacaagg ccttcttcgc
     4201 cggtcagtat cgtcattccg cgcgaaagcg acgcgccggt gagtttccat ggaatgtagt
     4261 cgctccggat ccattcctgc ttcttcgcgt cgaagatcgc ggcctcgttc tcgttccaga
     4321 cgagcgcgta ctcgtcggag agggacccca gaagcgtccg gaagtcgctc tccgagagcc
     4381 actgctgggt gcgcacgtcg tagacccagg ccgcatcctc gctccagacg agggcaagat
     4441 ggttgtggac gcggtatccg ctcgtacgga agtcgttcag gatggactgg cgtttcagca
     4501 ccgggtcgta cacccgcacg gtgtgtgcgt cgatgaagac ctgtacttca gcgaaaaccg
     4561 gaagcgccgt cgcgataagg agtatgcaga ggactgcgga aagtccgaga gatatccgtt
     4621 tcagtgtatt cataggatac cagctccctt catgtgtgtt tctgaacttc ttcatttatt
     4681 cccctattcc ccgacaagac ggagcgtgtt tccctcgacg gtctcctcct tcaggtccgg
     4741 gcgtttggcg aggagggctg cgaggagctg cccggccgaa ccggaatagg aaacgtcgat
     4801 ctccgccctg cctccggcga aacgggcgac cgaggcgttc gaagcgcccg cgtcgcggca
     4861 gcttgcggca agggcgtgcg cctcggcgaa cgaacgcgcc ggagcgatca ccaaaaacgt
     4921 tgctccggcc gaatccggtg gttgaggagg cgtcttgccg aggagcgacg ccgccgcgga
     4981 gcgtgttgca acctccacgg cttttcgcgc cgcctcgtcc gccgtgtacc ctatctcctt
     5041 tgcggaggcg cttccggcgg cgacctgacg cgcgttggaa gccgtgcacg ccgtcgccga
     5101 caccgtcgcg gtcgcggtga agaggccgaa ttcgttccgc accggagacg ggacggccac
     5161 ggagcccgaa agaaggacgg agaagccgaa tgtcctgccc agcgccataa tcgcctcgac
     5221 gttcccttcg agcgccagga tggccgcctt gttctggcgg atccgttcga gctgtttttc
     5281 gtcgacggcc ttgtaccctt catccagaag cgcccggatc agtatcgatc tcgcgagggg
     5341 ggcatgcgac gtccccttca caaggacggc gatagtcttg cccttcggca gttcggcccg
     5401 cgcctcgccg gacggaagaa gcgcaaagaa caagatgacg gccatgcaga ctgcgaacat
     5461 ttttttcatg gcatttacct cacatcggcg cgaacgattc tgctctcggt gaagaatcgc
     5521 ttcagcggaa ccgtcacctc gaccgtgtac acgccgtcct tcacgccctg aaaatcgacc
     5581 tgtccgtcga cgacttcgcc ctctatctca atggaggtga tcttctccgg aagtccgtga
     5641 cgaatctcat aaaggaggca gagaagattg cggcgcgcat ccgtcagcgc cccccgtttt
     5701 gccagcatct ggtcctgcgg ggaggttgaa ggaagagggg ccttcccctg cgacgtcacg
     5761 gactcgccct tccagtcgac gacgccgaag cgaaagacct gaatatcttc cgcaacgctc
     5821 ttcgactcga cgatggactg aaagagagcc gccaaatcct ccgttccctc cgcaggaagc
     5881 gacgcgaagg ctgcggagga gaagaaaacg accagaaggc acacaagtac tctgcagact
     5941 ctcaacgtct atcgcccctt tcgtccgttc accgtccctg cggtaatacc ggtgatttcc
     6001 acgccgagcg cctccagcgc gatggcgatc tcctccgaag aggtttccgc cgacacatcc
     6061 agttccagcg tccggttcgc gtaggcgcgc tggtagactc cgttgacgcc gcgtacctct
     6121 cccagactgt ctcgaagcga cctggcctcg gcgaatgaaa gatcgctcac ggtgaccttg
     6181 aacgatcttc cggggatact gccggaagcg ccgctgacga gcgcgtaggc gactgtgtgc
     6241 agcagcgagc cggccatgcg cggtgacagc ttttgaaacg ccttcattgc gccgtcgttc
     6301 ggcgacgtcc cctgcactct ctgttcggag ccctcggcgc cgatgatatg ggccgtctgg
     6361 gtgatgattg ctttgagctg aagctgcgag ttgacggcat agagcacgac gccggaaact
     6421 ttctgtttcg tcgctgtgct cgcctgcgcc tttccgtgga tcagcacgtc ggactgaaaa
     6481 ctccgtgcaa gctcctgaag cacggcagtg tcgcccgata ttttcgcggc ttcgatctct
     6541 cccttgcgga tcgcccctgc ctgtcccggg tcgacgagca tgtacccagc cttctgaaac
     6601 gctttcagta cttctccctc cgccgtggag aggaactgtc gctcgccgtc gacaacttcg
     6661 tcgatgagca ccatgatcct cggattgccc agcgcgtcga tcacagcggg gccgagaact
     6721 ccgtcgagcg tcgacgccga gacggtgcac tgcgcggtca ggcgaagaat gccgtcgtta
     6781 gtttgttctt ccttcaaatt ctccgtggct gttaccagcc cctccgaacg ggaaaagacc
     6841 gtgtcgcgga ccacctcgaa gtctttcatc tcggtgatgc cctgaacccg tgcgccgatt
     6901 cccttttcga gcgcgtctct gtagaggtga cgccgcgctt cctcccgcgc ccgagcgacg
     6961 tcgccgttcg caatcgcggc cattccctcc gcctcgacga cgatcttctc cgttccggag
     7021 gcgagcgccg ggagaaggag caggagaagc gcgcatgtgc acactacgga gaggagcgtc
     7081 cgtctcatgg cgtcaccgtt tgaccaccgt cacgcggcag ggaacgcgaa agtcgtacga
     7141 gctgccgcgc accttcgcag cgtccgcgtt gctgatgacg atatcgacgt tctgcggcgc
     7201 gaccgtccgc accgcttttg cgacgagagg tgtggaggcg attcgcggag cgcccttcgc
     7261 ataggcgagg tttgtgtggt agtcgcacag accggaggcg aggaagcgct ccgcgtcggc
     7321 aaagtcgatg ccgtacactt ccttgccgtt ttcgtcgacg acgcggaaga tcatcgccgg
     7381 gatgagaggg agatgcgtcg cgtcgatcag cagccccgat gcgctgatct tgcctgcggg
     7441 aggtgttggg ggaacgggtt tttgtcccgg agccttggga agtcccggca gcacggcggt
     7501 gcgcactttt tcgagtttca cgcgtcctgt gacggtgtag atctcgccgt cccaggaggc
     7561 gtcggaaatc tcgattccct tgatgagccc ctgaacctcc gtccgtacgg tatcgttcgc
     7621 catgaagtcg gtcatcgtcg tcttcgcgtc cacgcgcacg ccctggattg cctcaagaag
     7681 attgcgctgg aggtcgacga tggcccccct gcgcgcaagc agttttccct gcgcggtctt
     7741 ttccttgccg gtcggggcga cggcctgtcc cgaggcctcg acgtaatcgt cggtccagtt
     7801 gacgcgggcg aattcagtgc cgacgataag cgcggacggg gatggagcgg ggagcgttcc
     7861 agtatcgaat gcagtggaaa cgtcgaccgc cggcgcggcc ggcacggaat ctcctgcctc
     7921 aggcaggtct tcgatcggcg gaaggaacac ctcctccgtg gcaaacgccg gaactcctaa
     7981 gagcaacagg aacagaagac aagaaacgaa gcgtttttga gacatcgcat actcctcctc
     8041 tctagttttt cgaggaagat gggccgaggt ttatgtgcgg agagaaaaga aaatgtatac
     8101 cattctcgcg tcttcctcgt acactatgat actctattta cttcgaattt tcaaccgaag
     8161 cgcgatgttg ctttaatagg aaaaaaggct gaagcgcctt ccttaagaag cgcttcagcc
     8221 cgtaatttct cgcaggaggt ttttctggcg ctacgcttcc gcaggaagaa agtgcagccc
     8281 ctccgggaga aaacgcagct ccgcctccgc tccggggggg agcgggtcgc gctcgaacgg
     8341 gttcggaatg tgcaccgtaa ccggttcggt cccgggcagc tccacctcgt actccatcac
     8401 gcttcccaga taggtggcgc ggagcacggt cccggctatc gcgttcctgc cggagtcttc
     8461 gagaggggag aggcgcacgg cctcggggcg gatcatcgcc tcgtaggcgc cggccgtcag
     8521 ttcttctccg gcttcggggc gccacggttt ccccagaagc gtccacgcgc cggaagcgtc
     8581 tttttcggcc ggaagaaagg ccgctttgcc gatgaaggag gcgacaaagc ggttgatagg
     8641 gcgtgcgtac agctcccgcg ggcttcccat ctgttcgatg cgcccccggt tcatcacgac
     8701 gacgcggtcg gagatgctca ttgcctcgat ctggtcgtgc gtcacgtaga tgctcgtgat
     8761 gccgagacgc tgctgtaact tcctgatgtc gatgcgcatc tgttcgcgga gcttcgcgtc
     8821 gagattcgag agcggttcgt cgaagaggag gacggacggc tccatcacga cggcgcgcgc
     8881 cagcgccacc cgctgctgct gtcctcccga gagctggctc ggctggcgtt cctccagccc
     8941 ctccagcccg acgagctcca cgacgcgttc ggtccgctcg cggatctcct tcttgctcag
     9001 atccttcagc ttcaggccga aggcgatgtt gtcgcgcacg ttgaggtgcg ggaagatggc
     9061 gtacgactgg aagaccatcg tcgcgttccg cttgttcggc gcgatgtcgt tcatccgccg
     9121 ctccccgaag aagatgttcc ccgaggtagg atcctcgaag cccgcgagca tccggaggag
     9181 cgttgttttg ccgcatcccg agggaccgag aagagtgacc agctcgccgt cgcggacctc
     9241 cagcgagacg gcgtccaccg cccggaagat gctgcccgga tcgtcgaagc tgccgaaatc
     9301 cttggttata gagtcgatga ctattttcac gagcctattc ctcctgaatc tggattgccg
     9361 ccatgccgcc cgcgcggccg gggacgaggc gtgcgatgat tgccatcgcc gccgtcacga
     9421 tcaccacgag aatcacgctg aatgccgcag ccacgccgag acgccccgat cccacctggt
     9481 tcagtatctg caccgtgagc aggttccagt tggccgagac gaggaagatg gcggcgctga
     9541 tggcggtcat cgcgcggacg aaggcgaaga cgagccccga gaagaaggcc ggggcgatga
     9601 tcggcagcgt gacctttcgg aaggtggtca gcctgtccgc gccgaggttc tgcgcggcct
     9661 cctcgatgga cgggtcgatc tgacggagca ccgcgatccc cgactggatg ccgaccggga
     9721 tgtagcggaa gacgaagttc agcaccagga tcgcgagcgt gccgatcagc atgaggggcg
     9781 gcgtattgaa ggcgaggatg tacccgatgc cgatcacggt gccggggacg gcgaagttga
     9841 ggatggaggt gaactccatg acccccttgc cggggaacga ggtgcgcacc acgaggaagg
     9901 cgatcagcat ccccagcacg ccggagagcg gcgtggcgca gatcgcgacg atgagcgtgt
     9961 cgcggatggt ccccatgccg acgctgaagg cgtaggcgaa gtggtgcagc gtaggagtga
    10021 agtcgaaccc ccagacgcgg gtgaacgcgc cgacgaagat cgtcccgtag aagaggagga
    10081 cgaggccgca gaaggcggcg gagaaggcga tgaggccgcg ccgagcgttc ctgctcacca
    10141 gcttggagga ggaggcgacg ggctttccgg tcacggtggt gtactggcgc ttctgcaggt
    10201 agtagcgctg gatggcgaag gaggtgatcg acgggatcag cagcaccacc gcgagagcgg
    10261 ccccgagcgg caggttgaac gagccggtga tctcgaggta ggcctgggtg gagagcatgg
    10321 ggaagcggct tccggcgagc accagcgggt tgccgaagtc ggcgagcgac tctatgagca
    10381 ccacaaggaa ggcgctcgcg attcccggca gactcagcgg cagcgtcacg cgcagaaagg
    10441 tctggagcct ccccgcgccc acattcatcg ccgcgtcctc cagcgtcgga ttaagcgact
    10501 ccatgacgcc cttcagcgtg aggtaggcca ccggggcgaa ggtgaagatc tgcgacagca
    10561 gcacgccctt gaagccgtag atgttgtagt ttcggattcc cagcagcccc cgcgtgatca
    10621 ggccgttcgc gccgaagagc atcaggatgg agagcgcgct cgtgaagggg ggcgagatga
    10681 tggggaggat gaagagcagg tggagcgcct tcttccatgg gaccgacgtg cgcgtgatcg
    10741 tgaacgccgc cgcgtagccg acgagcacgc tgcacgcggc ggtgcagaag ccgacggtga
    10801 gactgttcca gagagacgag cgaaggtagt tgaacgtgag gaaacgcgta tagttctcga
    10861 gcgaaaacgc gccctgctcc gactgaaagc tcttgacgag cacggagagc agcgggaaga
    10921 cgacgaagac gcccagcgac acgaagagca gcactaccag aagcgcgaga agggggtcgc
    10981 gggcaagaag ctgaatctcc cttctcccgc gtgtgaatgc ggtcattttg ttgattgaca
    11041 cctcctaagc aaaaggggga agaggcaagc cccttccccc gcagctacaa cacaacgatt
    11101 actggccgag gacttccgtc acccagcggt cgacgaggcg cttcttctcg cgcgcggcct
    11161 caagacggtc gtagtcgatc agcttgatct cggagagcgg gggaagccct tcgcctgccg
    11221 agacgtcggt gcgcacgggg aagaagtagg tcttcttcgc gctgagcacc tgctgccctt
    11281 ccttggagac gatccagtcg acaagcgcct ttgcgctgtc gagattcttc gcccccttga
    11341 tgatggagag cgccgccgcc tcgtacccca cgccctcggc ggggacgacg cctttcgccg
    11401 ggtacccctc gtccaccagc ttcaggaacg ccggggtgaa ctgga
//
LOCUS       DAOPKU010000022        11312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_6021_length_11312_cov_5.169406, whole genome shotgun sequence.
ACCESSION   DAOPKU010000022 DAOPKU010000000
VERSION     DAOPKU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_11312_cov_5.169406"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /gene="yedE"
                     /locus_tag="PK849_01360"
     CDS             complement(<1..784)
                     /gene="yedE"
                     /locus_tag="PK849_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HPE64800.1"
                     /translation="MNSIQRLLLSRSGPAITGAVAGVLGAFLVFFGNPGFCFSCFTRG
                     LTNVAAGFCGVEAVLHLRPEIPAFVLGSFLSAFSAKEFRPRGGSSPLLRLLLGFFTMV
                     GAKIFLGCTWRAYARLAEGDWNALAGIAGVIAGVGAGVYFLRQGFTLGRAGKVSVRSG
                     ALLPLISCVLLAILLFRPVFFPASGPDMSLVSVALSVGVGLLVGWMAQKSRYCTVGAI
                     RNLIMARDAHLLYGIIAFLIASFTMNILLDQFMPGFDNQPLEH"
     gene            993..1430
                     /locus_tag="PK849_01365"
     CDS             993..1430
                     /locus_tag="PK849_01365"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE64801.1"
                     /translation="MFDTALVILIGLAGGVAVGLQSPLAGALGQRIGPTASSTILHLS
                     GLLLSLFLLWCRGGERIQEWYTAPWYMLAAGCLGVALFQTINVTLPRLGATAMVTLII
                     VGQLLTGVLIDAQGWLGTAARPFDMFRAAGVLLLLAGGWLIVR"
     gene            1492..2697
                     /locus_tag="PK849_01370"
     CDS             1492..2697
                     /locus_tag="PK849_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPE64802.1"
                     /translation="MADFRASAQQLHQQVTTWRRDLHRIPETGVDTPQTEAYICAELD
                     KIGVSYRKGLGRHGVVALVEGKHKNKVFAIRADMDGLPIKEETGLPFASQNGCMHACG
                     HDAHAAMGLGSVKLLHDVRDQIEGSVLVVFQPGEEGCTDGPGGAKRMLDDGAFDDPKP
                     DAMVGLHTGSIWKGFVPGEIGYRPGGIMACMDRFDILIKGKGAHGANPQGSVDPISIA
                     GQIICELQTIISREVDPQEPCVISLGEIHAGSAFNIIPGECRITGTVRAFNQEMREFL
                     ARRIGEVASAVASGMRGSVEFSYGWHGPSPVVNDPVMTEELRLAAEKIVGPEKVREIP
                     RPSMGGEDVAFFLDKAPGTFFFLPGCNEAKGQTWPHHNSRFDIDEDVLWIGPAVMATM
                     AFDWLKKQA"
     gene            complement(2741..3217)
                     /locus_tag="PK849_01375"
     CDS             complement(2741..3217)
                     /locus_tag="PK849_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPE64803.1"
                     /translation="MRNAVASIETYYERGMGFLFRLIDECPDELWGKKGGGFFYWQQL
                     YHAFYCVDYFLLPAGSEFPEKPYGSAVAMFRETLDTVPAKDEIREYGAMMKAKADAWI
                     AALDDGMLGDRHEGMSERRKTEVTNAGVLTSLCGHNMYHVGCLDSVLRENGLKGVY"
     gene            3408..4208
                     /locus_tag="PK849_01380"
     CDS             3408..4208
                     /locus_tag="PK849_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPE64804.1"
                     /translation="MIGDMFDVFFFDLDGVIYIGGETTPGAVSVLDALRSMGKTIRFL
                     TNNPTTRVRIADRLRGHGVAAEMDEIVTSGSATAKYLAGEGIGRAWVIGESGLHKEVE
                     LAGILPAGETDCEAVVVGWDDAATLATIRKAASAIRKGARFVATNIDRTFPSPDGPMM
                     AVGALVEAIRIGSGQEPVVVGKPFAPMFREALHSVGVPPQRVVMTGDTPEVDILGAHR
                     AGVTAILMGDAPVPGEGDFRRPDGRIFSLPDLLDPVKSVVRWTPPPTE"
     gene            complement(4272..5276)
                     /locus_tag="PK849_01385"
     CDS             complement(4272..5276)
                     /locus_tag="PK849_01385"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HPE64805.1"
                     /translation="MNLARFPRRRYTEGRTPLEFLERLSAKLGGPEIWIKRDDMLGFL
                     PGGNKTRKLEFLMADALAQGADAVITCGAVQSNHCRLTLAAARKEGLECHLVIEERVP
                     GSYSEQASGNNFLFHLMGVDSIRVVPAKSDMLAEMEKTADELRRAGKKPYVIPGGGSN
                     ALGALGYVACAEEILAQAFDKGVAFDGIFTTSGSAGTHAGLAVGLWANRSGIPLVGIN
                     ISRPNETQVPIVRKLAGETARLLDIEEPLPDDLIQCVDGYVGEGYSLPTKEMVETVAL
                     LARTESILLDPVYTGKAFAGMLDLLRRGFCGKPERVLFIHTGGVPALFHYQKYFDGAY
                     "
     gene            complement(5304..6242)
                     /locus_tag="PK849_01390"
     CDS             complement(5304..6242)
                     /locus_tag="PK849_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPE64806.1"
                     /translation="METLRTHALFFLCLALAVALALLPPTAPYAVGMALLLGAAVSNL
                     APGSAPPGLGKMRKLALNSAVVLFGFGLNIRQVISVGGQGFWQTAVSLAVIISLGFFL
                     LRFFKVEAATLKLVTFGTSICGGSAIAAVSSVMKVRDEDIGVAMGVVFLLNAVALVAF
                     PLLASLLPLTPEQYGVWCALSIHDTSSVVGAAAVLGDASLQTATIMKLTRTLWITPIV
                     FLISLRQGEKGKLSIPLFIVFFLLASVTASVLPFAPFFKTLASSGKVLMAVALYIVGF
                     GLHRSVLKKVGAQGMLFGMLLWAVSIVMGYLLASMS"
     gene            complement(6361..7260)
                     /locus_tag="PK849_01395"
     CDS             complement(6361..7260)
                     /locus_tag="PK849_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE64807.1"
                     /translation="MTAPRQESRAAGEPNACRAISGREALRALACIAHLIREIFPGDM
                     SFAVIEDDEYLVYVPGRTIDIKKKPGDKLTEGSAGHRCMMEKRKIVKEFKKEDSPFGF
                     PYVAASVPIVDASGKAVGCVITAENITRQETMRETAELLRASTGQIADSLQTMTGQME
                     EMRAAGEMLNSITSQAVSKVEGTEQVFGVIEGVAKQTNMLGLNASIEASRLGDQGKGF
                     GVVAEEVRKLAVRSSDSARQIKEILEYFKNSTIEINSSTDTLLHIIKEQSKIIETIAA
                     SSEEMASVSAMLEKLSSSLGQGS"
     gene            complement(7333..10599)
                     /locus_tag="PK849_01400"
     CDS             complement(7333..10599)
                     /locus_tag="PK849_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE64808.1"
                     /translation="MTDPVLVFLHLVAAGYLLFLCVLTLLPSAEETSLKGGPLPSAPL
                     FLFNAGWGVALSVRAFLAANSFQSTVYPLAVSLAVGGSLFLIKKKGRTGMLLRALLAG
                     GCASLFLPGTRLPGGALFLLAGGWLFIDRFPLRARLGLSSTGDAPGGARDRAFSMFFL
                     AEGLELLFEGSTPFPVLLSLFFVFTGIFLLARLTFRDRKFPSKVRRALLAGFALYGAL
                     SLVGGVGIARMERSFRESLLREGYSRLEMVRSKFLFFEMMGSALAKTVASDPTLVREG
                     HAPSFASDLQLRLLSRRLGATLVFLLDTEGTVVSTSEKLLEGMNFAFRSYFQDALQGR
                     SGLMYAVGKATMSAGAYFSRPLVDRLGTVLGIVVVKMDLEPVFGELFRTDRVVMHRQG
                     EILSGPKDFESGRLEILQEDFLTLRCMSGEFQGGGVRGPDGRMLPIVSLPLPGGGWEL
                     TKLLDPGSLIRYRRTLFAFYSLTSVLVLLLLLRYTQKNQLIGELKREVREREAAEQAE
                     RAARASAEQTNALLAEERNRAQMLAEQAEAASTAKSSFLANMSHEIRTPLNAVLGMIG
                     LLLDTKLDERQRHYAEVVQTSGDALLSLINDILDFSKIEADRLEMEHIGFNLQTILED
                     TVEMLSQRAEQKGLHFGFFVEDDVPRFLKGDPGRLRQILINLIGNAIKFTPSGEVILN
                     VMLGSEVRETVSIRCTVRDTGIGIPEDRLPALFSAFEQVDASTTRTFGGTGLGLAISK
                     RLTELMGGHIGVESRIGEGSTFWFSIPFPVVPESDVARTADPEFLQEACECEAPMGSR
                     GKILLVEDTPVNQQVAVALLARMGYMADVAGNGEEALTLLDSIPYDLVLMDIQMPVMD
                     GFETTALIRSREASEKRERIPIVAMTAHALSGYREKCIEAGMDDYVSKPILPKELETA
                     LRRHLKKRSAEREEEIAAEEVAEEVKAEKGTENVLETAESSQWEDIPLFDQADGAVRT
                     AGDVVFLGKLLLLFADSNAGEEGRLREFAGRGEWESAKHAAHSLKGSSGNLGLLRLAE
                     QARWTELALAEYMAGQSELDENALRSAVLSLADLASETMELARRQGTILGKEAEEAPS
                     "
     gene            complement(10631..>11312)
                     /locus_tag="PK849_01405"
     CDS             complement(10631..>11312)
                     /locus_tag="PK849_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HPE64809.1"
                     /translation="LVFTGLNRAVAEYDKGASFLDEKGLPAKNITGVFEYLFMKEQFE
                     MLEVLLQRPLRKVALLHSVDPVGVILKEQIADELSRTPYAGIVEFHAAEDAESALRLA
                     DAIGADDSVDAWIPVTMSVKDSEGGFLTMGDLAKEMTARIRKPDLALNVSFTELGFYG
                     GVSVDFYQMGFQTGSMAAKLLKGLPIREIPVENAGASIIAVNRSRLRELGISIPGDFS
                     AVVDLFLE"
BASE COUNT         2307 a         3546 c         3409 g         2050 t
ORIGIN      
        1 tgtgttccag aggctggttg tcgaatccgg gcatgaactg gtcgaggaga atattcattg
       61 tgaacgaggc aatcaggaag gctatgattc cgtaaaggag gtgggcatcc ctggccatta
      121 tcaggttacg gatggctcct acagtgcagt agcggctctt ttgcgccatc caccctacca
      181 gtaaacctac gccgactgag agcgccaccg aaaccaaaga catgtccggt ccggaggcgg
      241 ggaagaaaac cggtcgaaag aggagaatcg ccaacagaac gcacgatata agaggaagga
      301 gcgctcctga tcttacggag acctttccag ccctcccgag agtgaagccc tgcctcagaa
      361 agtagacgcc cgctccaacc ccggcaatca cccctgcgat acctgccagc gcattccagt
      421 cgccttcagc gagacgggca tacgcccgcc aggtacagcc aagaaatatc ttggcgccga
      481 ccatcgtaaa gaagccgagc agaagtcgaa gcagcggaga ggagccccct ctgggcctga
      541 actcttttgc ggaaaaagcc gaaagaaacg agcctaaaac gaaggccggt atctccggac
      601 gaagatggag cacggcttcg acgccgcaaa accccgccgc aacgtttgtc aaaccgcgtg
      661 tgaaacatga aaaacagaac cccgggttac cgaagaagac caggaaagcg ccaagcacac
      721 cggctacagc gccggtaatt gcggggccgg aacgggagag aagcagtctt tggatagagt
      781 tcatacgcaa cagccgcctt tcggatggtg cccacagcat catttttccc aaattgtacg
      841 ttatcttcca agttccagca agggaaactg gcatttcctc aagaacagat acaaaccctc
      901 ctctgccaaa cgtggagatc aacgcaccgg ccactgccgg cgtgatatga ttgtttccgt
      961 cctgaaaatg taacttttaa ggggagaacg ccatgttcga tacggctctt gtcattctga
     1021 tcgggttggc cgggggcgtc gccgtcggtc tgcaatcgcc gctggccggg gcattgggac
     1081 aacggatcgg ccccaccgcg agcagcacga tcctgcacct ctccggattg ctgctctcgc
     1141 tgtttctgct ctggtgcaga gggggagaac ggattcagga gtggtatacg gcgccgtggt
     1201 acatgcttgc cgcggggtgc ctgggagtgg cgctgttcca gacgatcaac gtaacgctcc
     1261 cccgcctggg cgcgacggcc atggtgacgc tgatcatcgt aggacagctg ttgacgggcg
     1321 tcctcatcga cgcgcagggg tggctgggta cggcggcgcg cccgttcgac atgttcaggg
     1381 cggcgggcgt gctcctgctg ctcgccggag gctggctgat cgtacggtag gcgtttttct
     1441 gcgttgtttt ttacgggaca tcaacgaaca aagagcaaag gagatggtgt aatggcggac
     1501 tttcgcgcat cagcgcagca gcttcatcag caggtaacaa catggcggcg ggatctgcac
     1561 agaatccccg agacgggagt ggacacaccg cagacggagg cgtatatctg cgccgagctg
     1621 gataaaatag gggtctccta ccgcaagggg ctcggcaggc acggcgtcgt cgcgctcgtc
     1681 gagggaaagc ataaaaacaa ggtcttcgct atccgcgcgg acatggacgg tcttcccatc
     1741 aaggaagaga cggggcttcc cttcgcctcg cagaacggct gtatgcacgc atgcgggcac
     1801 gacgcgcacg cggcgatggg actgggctcg gtgaagctgc tgcacgacgt cagggaccag
     1861 atcgagggct cggtactcgt cgtcttccag cccggcgaag aggggtgcac cgacggcccc
     1921 ggcggagcaa agcggatgct cgacgacgga gcgttcgacg atccgaagcc ggacgcgatg
     1981 gtcgggctgc ataccggctc catctggaag ggttttgtcc ccggtgagat cggctaccgc
     2041 cctggaggaa tcatggcctg catggaccgc ttcgatattc tgatcaaagg gaaaggggcg
     2101 catggagcaa accctcaagg ctccgtagat cccatctcta tcgcagggca gatcatctgc
     2161 gagttgcaga cgatcatcag ccgcgaggtc gaccctcagg agccgtgcgt gatcagcctg
     2221 ggcgagatcc acgcgggaag cgccttcaac atcatccccg gagagtgccg catcaccggc
     2281 acggtccggg ccttcaatca ggagatgcgg gagttccttg cccggcgaat cggcgaggtg
     2341 gcctcggcgg tggcctccgg aatgcggggg agcgtcgagt tctcgtacgg ctggcacggc
     2401 ccctcgcccg tggtgaacga ccccgtcatg acggaggagc ttcgcctggc ggcggaaaag
     2461 atcgtcggtc ccgagaaagt ccgggagatc ccccgcccct cgatgggcgg cgaggacgtc
     2521 gccttcttcc tcgacaaggc ccccggcacg ttcttcttcc tccccggctg caacgaggcg
     2581 aaggggcaga cgtggccgca ccacaacagc cgtttcgaca tcgacgagga cgtcctctgg
     2641 atcggaccgg ccgtcatggc cacgatggcc ttcgactggc tgaagaaaca ggcgtagttt
     2701 tcaaagaagg gagtcccgca acggactccc ttccgccctc ttaatatacg cccttcagcc
     2761 cgttctcccg cagcacagag tcaaggcagc cgacatggta catgttgtgg ccgcacagcg
     2821 acgtaaggac acccgcgttg gtcacttcgg tcttccgtcg ctcggacatg ccttcgtgcc
     2881 tgtcgccgag catgccgtcg tcgagagcgg cgatccatgc gtccgccttc gccttcatca
     2941 tcgcgccgta ctcgcgaatt tcatccttcg ccgggacggt gtcgagggtc tcgcggaaca
     3001 tggccaccgc gctgccgtac ggcttctccg gaaattcgct tcctgcgggg agcaggaagt
     3061 agtccacgca gtagaacgca tgatacagct gctgccagta gaagaaccct ccgcccttct
     3121 tcccccagag ctcgtcgggg cactcgtcga tcaaccggaa gagaaagccc attccccgct
     3181 cgtaataggt ctctatagac gctaccgcat ttctcatctt cattcctccc tttcgctgta
     3241 catactcggc agccgtttct ggacggctgc accccgaacg cattataaac caaacgaagg
     3301 tataggaaag aaccggaggg caatcgcacg cgcctttcca aaaaaaatgg aaaaatggta
     3361 caatgatttc cggtttttct cagtaattgc ggcgaaggag gatactcatg atcggcgaca
     3421 tgtttgatgt ttttttcttc gatcttgacg gtgtgatcta catcggcggt gagacgacgc
     3481 ccggagcggt gtcggttctc gacgccctgc ggagcatggg gaagacgatc cgattcctga
     3541 ccaacaaccc caccacgagg gtgcgcatcg ccgaccggct gcgcgggcac ggcgtcgcgg
     3601 cggagatgga cgagatcgtc acctccgggt cggccaccgc gaagtacctc gccggagagg
     3661 gaatcgggcg ggcgtgggtg atcggcgaat cggggcttca caaagaagtg gagcttgccg
     3721 ggattctccc cgcgggcgag accgactgcg aggcggtcgt cgtcggctgg gacgacgctg
     3781 caacccttgc gacgatccgc aaagcggcga gcgccatcag gaagggggcc cgcttcgtcg
     3841 ccacgaacat cgacaggaca ttcccctctc ccgacgggcc gatgatggca gtcggggcgc
     3901 ttgtcgaggc gattcgcatc ggctccgggc aggagcccgt cgtggtggga aagccgttcg
     3961 cgccgatgtt ccgcgaggcg ctgcactctg tgggcgttcc cccgcagcgc gtggtgatga
     4021 ccggcgacac gccggaggtg gatattctgg gagcgcaccg ggcgggcgtc accgccattc
     4081 tgatgggcga cgctcccgtc cccggagagg gcgacttccg ccgtccggac gggcggatct
     4141 tctcgctccc cgacctcctc gatccggtga aaagcgtcgt ccgatggacg cctcccccga
     4201 cggagtaacg cacacacaag aaagcgtccc ccgaaactga ggagggacgc tttcttgtgt
     4261 gtgcacgttt ttcagtatgc cccgtcgaag tacttctggt agtgaaagag cgcggggacg
     4321 ccgcccgtgt ggataaagag cacgcgctcc ggtttgccgc aaaagccccg ccgcagaaga
     4381 tcgagcattc cggcgaacgc cttcccggtg tacaccgggt cgagcaggat ggactccgtc
     4441 ctcgccagca gcgccacggt ctcgaccatc tccttggtgg gcagggagta cccctctccg
     4501 acgtagccgt cgacgcactg aatcaggtcg tccggcagcg gctcctcaat gtccagaaga
     4561 cgcgccgtct ctccggcaag tttccgtacg atcggaacct gcgtctcgtt ggggcggctg
     4621 atgttgatcc cgacaagggg gatgcccgac cggttcgccc acagtccgac ggcgagtccg
     4681 gcgtgcgttc cggcgctgcc gctggtggtg aagatgccgt cgaacgcaac ccctttgtcg
     4741 aacgcctggg ccaggatctc ctcggcgcag gcgacgtagc cgagcgcgcc gagcgcgttg
     4801 gaaccaccgc cgggaatgac gtagggcttt tttcccgcgc ggcgcagctc gtccgcggtc
     4861 ttttccatct cagcgagcat gtccgatttg gccggaacga cgcggatcga gtccactccc
     4921 atcaggtgaa agagaaagtt gttgccggaa gcctgttcgg agtagctccc cggaacgcgc
     4981 tcctctatga cgaggtggca ctcgagccct tccttcctcg cggcggcgag ggtgagacgg
     5041 cagtggttcg actgcaccgc gccgcaggtg atcacggcgt ctgcgccctg cgccaacgcg
     5101 tcggccataa ggaattcgag cttccgggtc ttgttccctc ccgggagaaa gccgagcatg
     5161 tcgtcccgct tgatccatat ctccgggccg ccgagctttg ctgaaagccg ttcgagaaat
     5221 tccaaagggg tccgcccctc ggtatagcgt cttctgggaa agcgtgcgag attcatctta
     5281 ttccatctcc tttgtgtcga atgtcagctc attgatgcga ggaggtaccc catgacgatg
     5341 gagaccgccc agagcagcat gccgaagagc atcccctgcg cgccgacctt cttgaggacg
     5401 ctgcggtgca ggccgaagcc gacgatatag agcgcaaccg ccatcagtac cttcccggac
     5461 gaagcgagcg tcttgaagaa cggggcgaag ggaaggacgg acgccgtcac cgaggccagc
     5521 agaaagaaga cgatgaagag cgggatggag agcttgccct tctcgccctg gcgcagcgag
     5581 atcaggaaga cgatgggcgt gatccagagg gtccgggtga gcttcatgat ggtcgcggtc
     5641 tgcagcgacg catctcccag caccgccgcc gcccccacaa ccgagctggt gtcgtggatg
     5701 ctcaacgcgc accagacgcc gtactgctcc ggcgtgagcg gaagcaacga cgcaagcagc
     5761 ggaaaggcga cgagcgccac ggcgttcagc agaaagacga cgcccatggc gacgccgatg
     5821 tcctcgtcgc gaaccttcat caccgaggac acggcggcaa tggcgcttcc gccgcagatg
     5881 gacgtgccga aggtcaccag cttcagcgtc gccgcctcga ccttgaaaaa ccggagcaga
     5941 aaaaaaccga gcgagatgat caccgcaagg ctgaccgcgg tctgccagaa cccctggccg
     6001 ccgacggaga tcacctgtcg gatgttcaga ccgaacccga agaggacgac ggcgctgttc
     6061 agcgcaagtt tgcgcatctt ccccagtccg ggaggcgccg agccgggggc gaggttgctc
     6121 accgccgcgc ccaggaggag cgccataccg acggcgtagg gcgcggtcgg aggaagcagc
     6181 gccagcgcca ctgcaagggc gaggcagaga aaaaagagcg cgtgggtgcg aagagtttcc
     6241 actacatatt cctccttggc agaaaacgca ggactcggga gaaggcaacg tctcgcgatc
     6301 ctgcgatgtt tttcaaaaag agcgggctga cggagaccgt ccttcccgcc gggacacgtc
     6361 tcagctccct tgcccgagcg aggaggagag cttctccagc atcgccgaga cggaggccat
     6421 ctcttcgctg gacgcggcga tggtctcgat gattttcgac tgttccttga tgatgtggag
     6481 cagggtgtcg gtgctgctgt tgatctcgat cgtggagttc ttgaagtact ccagaatctc
     6541 cttgatctgc cgtgctgagt cggacgaacg gacggcgagc ttccgcacct cctccgccac
     6601 gacgccgaac cccttcccct gatctcccag gcgggaggct tcgatcgagg cgttcagccc
     6661 gagcatattc gtctgcttcg ccacgccctc gatgacgccg aacacctgtt cggttccctc
     6721 caccttgctc acggcctgcg atgtgatgga gttcagcatc tcgcccgccg cgcgcatctc
     6781 ctccatctgt ccggtcatcg tctggagaga gtccgctatc tgccccgtgg aggcccgcag
     6841 cagttccgcg gtttcccgca tcgtctcctg acgggtgatg ttctccgccg tgatgacgca
     6901 gccaaccgcc tttccggaag cgtcgacgat cggcacggag gccgcgacgt acgggaagcc
     6961 gaacggcgaa tcctccttct tgaactcctt gacgatcttc cgtttttcca tcatgcaccg
     7021 gtggcccgcc gacccctcgg tcagcttgtc accgggtttc ttcttgatat cgatcgtcct
     7081 gcccggcaca tagacgagat actcgtcgtc ctcgatgacc gcgaaactca tatctcccgg
     7141 gaagatctcc cggataagat gcgctatgca ggcaagcgcg cgaagcgcct cccggccgct
     7201 gatcgcgcgg caggcgttcg gctctcctgc ggctcgactc tcttggcgcg gtgctgtcat
     7261 gattcatccc tccatcattc ggtcaatcct cgccgaaaag gatagcacaa ttcagcccgg
     7321 ccgcgtagag gatcaggagg gggcttcttc cgcctccttg cccagaattg ttccctgccg
     7381 ccgggccagc tccattgtct cggaggcgag atcggcgagg gagagaacgg cggaacgcag
     7441 ggcgttctca tccagttcgg attgccccgc catatattcg gcaagtgcca gttccgtcca
     7501 gcgtgcctgt tcggcgagac ggagaaggcc caggtttccg gaggaacctt tgagcgagtg
     7561 cgccgcgtgc ttcgctgatt cccactcccc gcgtccggca aactcgcgga gacgcccttc
     7621 ctccccggcg ttcgagtcgg cgaagagaag aaggagtttt cccagaaaaa cgacatcccc
     7681 ggcagtccgc acagcgccgt ctgcctgatc gaagagcggg atatcctccc actgcgaact
     7741 ctctgccgtc tcaaggacgt tttcagttcc tttttccgcc ttcacctctt ccgcaacctc
     7801 ctctgctgca atttcttctt cgcgttccgc ggatcgtttt ttcaaatgcc gccggagcgc
     7861 tgtctccagc tccttcggca atatgggttt gctcacgtag tcgtccattc ccgcctcgat
     7921 gcacttctcg cggtatcctg agagcgcgtg cgccgtcatc gcgacgatcg gaattctctc
     7981 tctcttttcg gacgcttctc gggagcggat gagcgcagtc gtctcaaatc cgtccatgac
     8041 gggcatctga atatccatca ggaccagatc gtaggggatg gagtcaagaa gcgtcagcgc
     8101 ttcctcgccg ttccccgcga cgtcggccat gtaccccatc ctggcgagga gcgcgacggc
     8161 gacctgctga ttcaccggag tatcctccac gagaagtatt ttcccccttg aacccatcgg
     8221 cgcttcacac tcgcaagcct cctgcaggaa ctcagggtcc gccgtccgcg caacgtcgct
     8281 ctccgggacg accggaaacg ggatggaaaa ccagaaggtg gagccctcgc cgatgcggct
     8341 ttccacacct atatgtccgc ccatcagctc cgtcaggcgc ttgctgatgg ccagccccag
     8401 ccctgtgccg ccgaaagtac gggttgtgga ggcgtccacc tgctcgaagg cgctgaaaag
     8461 cgccggaagg cgatcctccg ggataccgat ccccgtgtcg cggacggtac agcgtatcga
     8521 gaccgtttca cgaacctccg acccgagcat cacattgagg atcacttcgc ctgagggagt
     8581 gaacttgatc gcgttgccta tcaggttgat cagtatctgg cggaggcgtc cgggatcccc
     8641 tttcaagaaa cgcgggacgt cgtcctcgac gaagaaaccg aaatgcagcc ccttctgctc
     8701 ggctctttgg gagagcatct ccaccgtatc ctcaaggatc gtctgcagat tgaacccgat
     8761 atgctccatc tccagacggt ccgcctcgat cttcgagaag tcgaggatat cgttgatcag
     8821 cgagagcagc gcgtccccgc tcgtctgtac gacttcggcg tagtggcgct gtcgttcgtc
     8881 gagcttcgta tcgagcagaa ggccgatcat gccaagcacg gcgttcagcg gagtgcgtat
     8941 ctcgtggctc atgttggcga ggaagctgct cttcgcagtg ctggcggctt cagcctgttc
     9001 cgcaagcatc tgcgcccggt tgcgctcctc cgcaagaagg gcgttcgtct gttccgcgga
     9061 ggccctggcc gcgcgctccg cctgttccgc cgcctcccgt tcgcggacct cccgtttcag
     9121 ctcgccgatg agctggttct tctgcgtata acgcagcagc agcagcagca caaggacgga
     9181 ggtgagcgaa taaaaggcga aaagcgttct tcggtatcgg atgagagagc ccgggtcgag
     9241 cagttttgtc agctcccagc cacccccggg aagcggcagc gaaacaatcg gcagcattcg
     9301 cccatccggg cctcgcaccc ctcccccctg aaattcgccg gacatgcacc gcagcgttaa
     9361 aaagtcttcc tgaagaattt caaggcggcc gctctcgaaa tccttcggcc ccgagagaat
     9421 ctccccctga cgatgcatca caaccctgtc tgtgcggaaa agctcgccga agaccggttc
     9481 caggtccatc ttgaccacga cgataccgag gaccgtcccc aacctgtcca caaggggacg
     9541 ggagaagtac gcccccgcgg acatcgtggc ctttccgacc gcgtacatca ggccgcttct
     9601 tccctgaaga gcgtcctgaa agtacgaacg gaaggcgaag ttcattcctt caagcagttt
     9661 ctcggatgtg gagacgaccg ttccctccgt atcaagcaga aagacgaggg tggcgcccaa
     9721 cctccggctc agcagacgaa gctgaagatc cgaggcgaac gacggcgcgt gcccctccct
     9781 gaccagcgtg gggtcggagg cgaccgtctt cgcaagagcc gatcccatca tctcgaagaa
     9841 gagaaacttg cttctcacca tctcaagccg gctgtacccc tcgcggagaa gcgactccct
     9901 gaacgagcgc tccatccgcg cgatcccgac gcctccgacg agcgacaagg cgccgtacag
     9961 cgcaaaaccc gcgagcagcg ccctccgtac cttggagggg aacttcctgt cgcgaaaggt
    10021 cagccgggcg agcaagaaga ttcctgtaaa gacgaagaaa agagaaagaa gaaccgggaa
    10081 cggcgtgctg ccttcaaaga ggagctccag cccctcggcg agaaaaaaca tcgagaacgc
    10141 cctgtcgcgc gcgcccccgg gagcatctcc ggtcgaggag aggccgaggc gcgcgcgcag
    10201 cgggaaacgg tcgatgaaaa gccatccccc cgccagaagg aaaagcgcgc cccccgggag
    10261 cctggttccc ggcagaaaaa gagacgcgca gcctcctgcg agcagcgccc gtaggagcat
    10321 tcctgtacgc ccctttttct tgatgaggaa aaggctcccc ccaaccgcca gcgagacggc
    10381 cagcggatag accgtgctct gaaaggaatt ggcggccagg aaggcgcgga cgctgagcgc
    10441 gacgccccac cccgcattga agaggaagag aggcgcgctt ggaagaggcc ctcccttgag
    10501 ggaggtctcc tcggcagatg gaagaagcgt caggacgcag aggaaaagaa ggtaccccgc
    10561 tgcgacaaga tgaagaaaca ccagaaccgg atccgtcatc ccgccctcct tcggagccga
    10621 aaaaaacgac tcattccagg aatagatcga cgacagcgga aaaatcgccg ggaattgaga
    10681 tccccagctc gcgcagacgg ctcctgttga cggcaatgat cgaagcgcct gcgttctcca
    10741 ccggaatttc ccgtatcgga agtcccttca ggagcttggc ggccatcgag ccggtctgga
    10801 accccatctg gtaaaagtcc acgctgacgc cgccgtagaa ccccagctcg gtgaaagaga
    10861 cgttgagcgc aaggtcaggc ttgcggatgc gcgccgtcat ctccttcgcc agatctccca
    10921 tggtgagaaa tccgccttcc gagtccttca ccgacatcgt caccggaatc cacgcgtcga
    10981 cggagtcgtc ggcgccgatg gcatcggcaa ggcgaagcgc gctctccgcg tcctccgcgg
    11041 cgtgaaactc cacgatgccg gcgtaagggg tgcgggagag ctcgtcggca atctgctcct
    11101 tcaaaataac gcccacggga tcgaccgagt gcaggagcgc caccttgcgc agcggccgct
    11161 ggaggagcac ttccagcatc tcgaactgct ccttcatgaa gagatattca aagacgccgg
    11221 tgatattctt cgcaggaagc cccttctcat caaggaagga agcgccctta tcatactccg
    11281 caaccgcacg gttcagtccg gtgaacacaa ga
//
LOCUS       DAOPKU010000023        11139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_6147_length_11139_cov_4.377752, whole genome shotgun sequence.
ACCESSION   DAOPKU010000023 DAOPKU010000000
VERSION     DAOPKU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11139
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6147_length_11139_cov_4.377752"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..2596
                     /locus_tag="PK849_01410"
     CDS             320..2596
                     /locus_tag="PK849_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPE64810.1"
                     /translation="MTERLNVVGANVPRHDALAKVTGRARYVADLPCDGAWVGGALWS
                     RTPRARVRNIVFSPDFDWSRVTVITAPDLPGPNEVHMIKDDYPILAGDEISYIGQAIA
                     LVAAPDKQTIEEALGAMRPDLEELPPVLSVEASLAKEAVIWGEDNVQAHYRIEHGDLE
                     EAFSRSDVIIEETYRTHHQEHLYLETNGMFARPRPDGGVIVEGSLQCPYYVARALAHA
                     LGLPLEKVTMRQTETGGAFGGKEDYPSVLAIQTALLSMKSGKPVRMVLERREDIDVTT
                     KRHPSVIRHRTGVTRDGVILAAEIDIIFDGGAFSTMSPVVLSRGVLHAWSAYRLPNAR
                     IVGRALATNTAPNGAFRGFGAPQTVFAMERQMDRIARELGLSPLEVRRKNLLKTGDTL
                     PCGQVLRSAYAGLVLERALDISGYEEKHGKQQEDDRILRGVGVSVFLHGGGFTGAGEE
                     KISGRAAVEVADDGIVEVLVSSTEMGQGAATVLPQIAAEVLSLPAWKVRHPNPDTSRV
                     PDSGPTVASRTTMMVGKILQDAAKDMRATLTGEAAKNAGVLPESILWENGRLASGGRD
                     LGSFEELALQYKEEHGKPLRGEGVYNPPSDCQWNEAEYRGDAYKDYAWACDVVEVEID
                     ADTLELRVPHVTSVVEIGKAVHPMLAEGQVIGGTLQALGWGAMEEVRMEKGRYRNNSV
                     STYIIPTAVDAPEFTVEIAETPSPFGPWGAKGLGELPMDGGAPALAAAVEDALGVFAT
                     ELPLTSDRLHALLRQRRK"
     gene            2609..3979
                     /locus_tag="PK849_01415"
     CDS             2609..3979
                     /locus_tag="PK849_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPE64811.1"
                     /translation="MKFELTVNGKVCSVDTSPMRRLLDILRQDLGLPGAKEGCGEGEC
                     GACAVLLDGRLVNSCLIPAFQLPGRSVETIEALSVDGTPDTLQQAFLDAGAVQCGFCT
                     PGLVMASRALLSKNAHPTRKEIREGIAGNLCRCTGYEAVVHAVEQAAEKGYAPRLVPA
                     KRLDASVLLEKEEEGKVFLPRTLEEAFEALSVHGDELAVVAGGTDLMVDMEKKGLPAP
                     EKVLDITGIEGMRRIGVSGDWLEIGAGATFAEIAVHENVLSVAPALAHCASQVGAVAI
                     QNRATIGGNIATASAAADSPPVLMALGAVLVLASGKGSREMPVEEFFSEYRRTARRAD
                     ELLVTVRIPLSVRGTRQMFRKVGLRQALAISRVSVACVAALESGVVTSCRIAAGSMSP
                     TPLLLKDVCSAVTGRALSRELAEEAGRVADSLVSPRTAPEYRKETTGNLVRRFFTDIL
                     KENATV"
     gene            4203..4406
                     /locus_tag="PK849_01420"
     CDS             4203..4406
                     /locus_tag="PK849_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPE64812.1"
                     /translation="MTTQGTVKWFNATKGYGFVTCDDGKDVFVHYSAIQGDGFKTLDE
                     GQRISFRITQGSKGDQAADVEKI"
     gene            4634..5977
                     /gene="tig"
                     /locus_tag="PK849_01425"
     CDS             4634..5977
                     /gene="tig"
                     /locus_tag="PK849_01425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPE64813.1"
                     /translation="MRSEILSQENNVVSIKLEFEASEFAQGVEKAIKDLSTKVNIAGF
                     RKGHVPRKVMEMRFGRQAIYAEAIEAMLPDAIEQIVKDYDLDLIDEPHVSIEKMEEGS
                     PVDVRLTFEVSPEVVLPEVSAVTVHRPVASVADEIVSGTIEDIRAQNAEHSPVENVPV
                     AENHVVDIEYHTVVLLDEGKQERHGPDSTTLDLGQPSVRTEIRDALLGAKKGESRTAD
                     VQVEENYQDPKVAGRTVRYELTVNEIKEKVLPEMTPDFFKKILRSDCETEELFREEIK
                     KRILEKIASEIQGKAEFDALQGISDLSQLTVPETLISRQMTAMKKEDEENIKKSRNMS
                     LEEMLSEASTTMEEYEKNIREQAETIVRRSLVLDKVADEMGITVEKEDFEAEMQTLAS
                     SYKIDAQRLVEGLFKDEKRLMEIANRIKYKKTMKAIMNTVQIDETEPAAEEKPAE"
     gene            6010..6588
                     /gene="clpP"
                     /locus_tag="PK849_01430"
     CDS             6010..6588
                     /gene="clpP"
                     /locus_tag="PK849_01430"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPE64814.1"
                     /translation="MLVPYVVEQTGRGERTYDIYSRLLKDRIIFLGDSVDEHTGNIVV
                     AQLLFLESEDPEKDIHLYINSPGGYVSSGLAIYDTMQYIKCPVSTICLGHAASMAAVL
                     LAGGATGKRFVLPNARIMIHQPLGGVQGQATEVEIHAREILKLRERLNDILVKHTGQS
                     KKKVRADTERDFYMSPEEAVEYGIVDKVIHSR"
     gene            6813..8123
                     /gene="clpX"
                     /locus_tag="PK849_01435"
     CDS             6813..8123
                     /gene="clpX"
                     /locus_tag="PK849_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPE64815.1"
                     /translation="MFRFDDRGRGSKSNGGKNLHNGRCSFCGKGQDEVQKLIAGPGVY
                     ICDECIHLCNMILVEEAGETAAKAKTAEPVFPLSEVPKPREIKEYLDQYVIGQDLAKK
                     AVSVAVYNHFKRISNNMAEESDVELQKSNVLLMGPTGCGKTLIAQSLAKKLNVPFAMA
                     DATTLTEAGYVGEDVENILVKLLQAADYDVKAAQRGIIYIDEIDKITRKSESASITRD
                     VSGEGVQQALLKILEGTLSNVPPKGGRKHPYQDFIQIDTSNILFICGGAFEGIEEIIG
                     RRVNKKIIGFGGDIMGKHDARRFNLMKQIQPEDLMAFGFIPEIAGRLPLLVPLEELNT
                     DALIRILVEPKNSIVRQYQKTFSMEGVSLEFTEDALRSIALKAGEKNTGARGLRAILE
                     NLMMDLMYDIPSRGKDVGKVIITKEAVEGSQPPLEEARSSEEVA"
     gene            8128..10515
                     /gene="lon"
                     /locus_tag="PK849_01440"
     CDS             8128..10515
                     /gene="lon"
                     /locus_tag="PK849_01440"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPE64816.1"
                     /translation="MKKEEEAQAQDIQDITEVSASGPRSCFVLPVRDIVIFPGIIAPL
                     FVGRPRSLKAIEMAMLQDRNVLVVAQKEMQVDDPRVEDLYSVGTVCSILQMVRIPDGT
                     TKVLIEGVERVQVVSYGKGKETLEADVVSLPWEEHHSPNLEALKRSVLEQFEKYVTLH
                     PRIPGEVLISVMNVEDPRQISDLVGSHLSLKVEKKQKLLEMVNLEKSLKFLLKVLLEE
                     IDILQLEHAIQDKVRQEVERGHKEYYLREQLKVIQDELGQGEKPSEIDELRTRIEEAE
                     MPEASLSKALHELDRLSKMPLMSAEATVVRTYIDWLVTLPWNTMTPDNLEIKRAQKVL
                     DEDHYGLEKVKERILEFLAVRKLALDKMKGQVLCFVGPPGVGKTSLGKSIAKALERKF
                     VNMSLGGMRDEAEIRGHRRTYVGAMPGRILQKIRQAGARNPVLLMDEIDKLGLDFRGD
                     PAAALLEVLDPEQNSSFTDHFMEVPFDLSDVIFITTANVTHSIPRPLLDRMEVIKIPG
                     YVWQEKIKIAKNHIFPRILEEHGLTPKQLKITPSGLEHVISEYTREAGVRGLERELST
                     ICRKVARRIVEDEKNAEEPVSIGPKHLKEYLGPPRLYDPRLPKTKEIGTVVGLAWTET
                     GGDVLVIEAVTMKGKGEVTLTGNLGSIMQESAQTAIGFLRSNADGFGIGNIDWKAIDV
                     HLHVPEGAIPKDGPSAGITMAVAILSAVRKSSVLPNIAMTGEVSLQGKVLPVGGIRDK
                     ILAAKRQGIKNIILPLANKPDVEEIPEWSRDGLSFRYVDRVENVFEYALERAPSP"
     gene            10512..11111
                     /gene="yihA"
                     /locus_tag="PK849_01445"
     CDS             10512..11111
                     /gene="yihA"
                     /locus_tag="PK849_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPE64817.1"
                     /translation="MNSKWKVSLECTAFTADQFPPPEIPEIAVAGRSNVGKSTLINNL
                     LGARLAHVGATPGKTRSVNFFLVEREGVPFRLVDLPGFGYASRSKAERNTWSRLIESY
                     VASRKSLWLVLHLVDFRHGLLENDASLQEWLDGIGLPVFVVFTKGDKIAKGKHLSTRD
                     QFIRKGMKSIDVPIITSGDKGTGIQELRVFIESFLDRQE"
BASE COUNT         2672 a         2808 c         3337 g         2322 t
ORIGIN      
        1 ggaggcaaca tcaacgggca ccttctcttc ggcgtttgcg gcaggcacgt cagcaccaca
       61 atagccaacg ggaaagtcct gatggacgag cggaaacttg tccacgtgga cgaggaaaaa
      121 ataatggccg agagccgcgc tctggccaaa aaactctggg agcggattta acgaaaagac
      181 gatcgttgcc ggaggggtga gggagcgccc tttgaagcga ctatgcgtcg cgccggaggg
      241 cgcttcctca cgggctgtac ctgtcggcgg cgttcagtct tctcgtagat aatcccattt
      301 ttaaggagga tgcatgccca tgaccgaaag gctgaacgtg gtcggcgcaa atgtgccgcg
      361 acatgacgcc cttgcgaaag taacaggccg cgcccgctac gtcgccgatc tgccctgcga
      421 tggcgcatgg gttgggggag cgctgtggag cagaactccg cgtgcccggg tgcgaaatat
      481 cgtattttcc cccgattttg actggtcacg cgtgacggtg attaccgcgc ccgatttacc
      541 cggccccaac gaagtgcaca tgatcaagga cgactatccg atcctcgccg gcgacgaaat
      601 ctcctatatt ggtcaggcga tagctttggt tgccgcgccc gataaacaaa ccattgaaga
      661 ggcgctcggc gcgatgcggc cggatctgga ggaactcccc ccggtgctca gtgtggaggc
      721 ttctcttgca aaagaagccg ttatctgggg cgaggacaat gtccaggcgc actaccggat
      781 agagcacgga gacctcgaag aggcgttttc ccgttcggat gtgataatcg aagagaccta
      841 cagaacgcat catcaggagc atctgtacct tgagacaaac ggtatgtttg cccgccctcg
      901 accggatggc ggggtcatcg tcgaaggttc tcttcagtgc ccgtactacg ttgccagagc
      961 gcttgcgcat gccttggggc ttcctctgga aaaggtgacg atgcgccaga ccgagaccgg
     1021 cggcgctttc ggcggtaagg aggactatcc ctcggtattg gccattcaga ctgcgctgct
     1081 ttccatgaaa agcggaaaac ccgtacgaat ggtgcttgaa cgcagagagg atatcgacgt
     1141 caccacaaag cgccacccgt ccgtcatccg ccatcgcacg ggagtcacgc gcgacggcgt
     1201 tatcctcgca gctgaaatag acatcatctt tgacggcggc gcgttctcca ccatgagccc
     1261 ggtggttctc tcacgaggcg tgctccatgc gtggagcgcc tacaggcttc ccaatgcccg
     1321 tattgtcggc agggcgctgg cgacgaatac cgcccccaac ggagcgttcc gcggtttcgg
     1381 cgctccgcag actgtttttg ccatggaacg tcagatggac aggatagccc gcgagcttgg
     1441 gctctctccg cttgaagtgc gacggaaaaa ccttttgaaa acaggagata cgctcccgtg
     1501 cggccaggtg ctgcgttcgg cctatgccgg ccttgtgctc gaaagggcgc tcgacatatc
     1561 cggatacgag gaaaaacatg gaaagcagca agaggatgac cgcattctgc gcggggtagg
     1621 cgtctccgtc tttctgcacg gcggaggatt caccggggcg ggggaggaga agatctccgg
     1681 ccgcgccgct gtggaagtag ccgacgacgg aattgtcgag gtgctcgtca gcagcaccga
     1741 gatgggccag ggcgccgcga cggtgcttcc gcagatagcg gcggaagtcc tctctcttcc
     1801 agcctggaag gtgcggcatc cgaatcccga tacttcgcgc gtacccgaca gcggcccgac
     1861 agtcgcttca cgaacgacca tgatggttgg gaaaatactc caggacgccg cgaaggatat
     1921 gcgcgccacg cttaccggcg aggcggcgaa aaatgccgga gtacttcctg aatccatcct
     1981 ctgggagaac ggacgccttg cttccggggg gagagatctg ggctcttttg aggaacttgc
     2041 cttgcagtac aaggaggagc acgggaagcc tctgagaggg gaaggggtgt ataatccccc
     2101 ctccgactgc cagtggaacg aggcagagta ccgcggcgac gcatacaaag attacgcctg
     2161 ggcgtgcgat gtggtggaag tggagatcga cgcggatacg ctggagcttc gcgtcccgca
     2221 cgttacctcc gttgttgaaa tcggcaaggc ggtgcaccct atgcttgctg aagggcaggt
     2281 tatcggcggg acgcttcagg cgcttggctg gggcgcgatg gaggaggtcc gaatggagaa
     2341 gggacgctac cgcaacaata gcgtgtcgac ctacattatt cccacggccg tggatgcgcc
     2401 tgaattcacc gtggagatcg ccgagacgcc gtctcctttc ggtccctggg gcgccaaagg
     2461 attgggcgag ctcccgatgg acggcggcgc gcctgccttg gccgccgccg tcgaggatgc
     2521 tcttggcgtc ttcgcaacgg agcttccgtt gaccagcgac cgtttgcatg ctctgttgcg
     2581 gcaacgccgg aagtaaggag ggaactctat gaagtttgaa ctcactgtaa acggtaaggt
     2641 ctgttctgtg gacacgtctc caatgcgccg tttgcttgat attcttcgcc aggacctagg
     2701 gctgccgggc gccaaggagg ggtgcggcga gggggaatgc ggagcatgcg ccgtgcttct
     2761 cgacggccgt cttgtaaact cttgccttat ccccgctttc cagctgccgg gcagaagtgt
     2821 ggagactata gaagcgctct ccgtggatgg tacgcccgat acactgcagc aggcctttct
     2881 tgatgccggt gcggtgcagt gcggtttttg cactcctggt ttggtcatgg catcgagagc
     2941 gcttctgtcg aagaacgcac accctacacg taaagaaatt cgcgaaggca tcgccggaaa
     3001 tctctgccgc tgtaccgggt atgaagcggt ggtgcacgct gtggaacagg ctgcggaaaa
     3061 aggctacgca ccccgtcttg ttccggcgaa gcgtctggat gcctccgtgc ttctcgaaaa
     3121 agaagaagag ggaaaagtgt tcctccctcg aactcttgaa gaggcctttg aggcgctgtc
     3181 cgttcacgga gatgagctcg ccgtggtcgc gggcggcacc gacctgatgg tggatatgga
     3241 gaaaaaaggg cttcccgcgc ctgaaaaagt tctcgatatc acgggaatcg aggggatgcg
     3301 tcgcataggc gtttccggtg attggctgga gataggcgcg ggcgcgacct tcgctgaaat
     3361 cgcagtgcat gagaatgtcc tctccgtagc tccggctctg gcgcactgcg cttcacaggt
     3421 gggcgccgtc gcaattcaga atcgggcaac catcggaggc aacatcgcta ccgcttcggc
     3481 ggcagcggac tctccacccg tcttaatggc cctcggcgcc gtccttgttt tggcaagcgg
     3541 gaaaggatcg cgtgaaatgc cggtagagga atttttctcg gaatacagac gcactgcccg
     3601 gcgtgcagac gaactgctgg tcacggtgcg tatccctctt tccgtgagag gaacccgtca
     3661 aatgttccgg aaggtgggtc tccggcaggc gcttgccatt tcacgggttt ccgttgcctg
     3721 cgtcgcagcg ctggagagcg gcgttgtgac ttcgtgccga atagctgcgg gcagtatgtc
     3781 gccgacgccc ctgctcctga aggacgtctg ctcggcagtc acaggcagag ctctgagcag
     3841 agagctggcg gaagaggccg gaagagtggc cgattcgctg gtgtctcccc gtaccgcgcc
     3901 ggaatacagg aaggaaacta ccggaaattt agtacgacgt tttttcaccg atattctcaa
     3961 ggagaacgcg acggtctgat cgatccggag caggccgcaa acggcagagc gaaaaggagc
     4021 cggcagagtg gcattttttg ttttctcgtg cggattccgg aagaaacgca ggatgtaacg
     4081 aaaagaaagt gcgaatttaa agcaagaaag acttgacatg ctcttcattt cttattatga
     4141 tgtatgcgtt gacaggcgcc gtcagcaaaa agcacaagca atcaagctta ggaggaacaa
     4201 ctttgacaac tcaaggcaca gtaaaatggt tcaacgcgac gaagggttat ggattcgtaa
     4261 cctgcgacga tggcaaggac gttttcgttc attatagcgc tatccaggga gatggattca
     4321 agactctcga cgagggccag cggatttcct tccgtatcac tcagggttcc aagggagatc
     4381 aggctgccga cgtagagaag atataacttc gttcgacata tcgccatttc caaggcagcg
     4441 gttttccgct gcctttttct tttatttttg gcgactactt tgctttgcca gaaagagagc
     4501 ctgcatttct ttgcattctc tcagtgttcc gtgtacaata ccttccgcat attttctctt
     4561 cctgtaggaa tgagatacat tatagagaaa aaattacacc agtgcgaatc gcactgaaag
     4621 gggttgcgat tgtatgagat ccgagattct ttcccaggag aataatgtcg tctccatcaa
     4681 acttgagttc gaagcatcgg agttcgccca aggagtagaa aaggcgataa aagacctgtc
     4741 gacaaaggtg aacatagctg gtttccgtaa agggcacgtg ccgcgcaaag ttatggagat
     4801 gcgtttcggc aggcaggcga tctatgccga ggcgatcgaa gcgatgcttc ccgatgcaat
     4861 agagcagatc gtgaaagatt atgatctcga tctcattgac gaacctcatg tatcgattga
     4921 aaagatggaa gaggggagtc ctgtggacgt ccgcctgacc ttcgaagttt ctccggaggt
     4981 agttctcccg gaggtttcgg ctgtcaccgt acatcgtccc gtcgcttctg ttgccgacga
     5041 aatcgtctcc ggcacaatag aggacatacg agcgcagaac gcggagcact ctcctgtgga
     5101 gaacgttccg gttgctgaaa atcatgttgt ggatatcgag tatcataccg ttgtgctgct
     5161 tgatgaaggg aaacaggagc gccacgggcc ggattcaaca acgctcgatc tggggcagcc
     5221 ctctgtacgc accgagatcc gagatgcgct gctcggcgcg aagaaaggcg agtccagaac
     5281 tgcggatgtc caggtggagg agaattatca ggatcccaaa gtcgcaggac gtaccgtacg
     5341 gtatgaactg accgtcaatg agattaagga gaaagtcctt cccgagatga cgcctgattt
     5401 cttcaagaag atactcagga gcgactgcga gacagaagaa cttttccgcg aggagataaa
     5461 gaagcgcatc ctggagaaaa tagcctcgga gattcagggg aaagccgagt ttgatgcgtt
     5521 gcagggcatc tccgatcttt ctcagcttac ggttccggag actctgattt cccgccagat
     5581 gacggcgatg aagaaagagg acgaagagaa cattaaaaag agccggaata tgtcgcttga
     5641 agagatgctc tccgaggctt ccacgacgat ggaagagtac gagaaaaata tccgcgaaca
     5701 ggctgagacc atcgtccgca gatcgctggt gctcgacaag gtcgccgatg aaatgggaat
     5761 caccgtggaa aaagaagact ttgaagcgga gatgcagacg ctcgcctcat cgtacaaaat
     5821 cgatgcgcaa cgccttgttg aagggctctt caaagatgaa aaaaggctga tggagatagc
     5881 gaacaggata aaatacaaaa agacgatgaa ggctatcatg aatactgtac agatcgacga
     5941 aacggagcct gcggcggaag agaagcccgc tgaataggaa caacgctacg atcacaggag
     6001 gtgttccctt tgttggttcc atatgttgta gaacagaccg ggcgaggcga acggacatat
     6061 gatatataca gccgtcttct caaggacaga attatcttcc ttggggattc ggtcgatgaa
     6121 catacaggca atatcgttgt tgcacagctg ctttttcttg agagtgaaga tcccgagaag
     6181 gacattcatc tctatatcaa cagcccggga gggtatgtct cctcgggact cgctatttac
     6241 gatacgatgc agtatattaa atgccctgtt tccacgatct gccttggaca tgccgcgagt
     6301 atggccgcgg ttcttcttgc aggtggagcg acgggaaaac gctttgtttt gccgaacgca
     6361 cgcatcatga tccaccagcc tctcggaggc gtgcaggggc aggcgacgga agtggaaatt
     6421 cacgcacgag aaatactgaa gctgcgtgaa aggctcaatg atatacttgt gaagcacacg
     6481 ggacagtcga agaagaaagt acgggccgat acggaaagag atttctacat gtctccggaa
     6541 gaagctgttg agtacgggat cgtcgataaa gtcatccatt cgagatagag cgcgcttcag
     6601 ttccggaaac aactgttttg attcgacttc aagggagccc gagacaagca ggcaacgctc
     6661 cggcgcgttt atggaaacgc accgtcggca tagagggaaa atacccttct tgcccgggag
     6721 tttgtcggct tgcattcctg tacaggggat gggagttcag gcgctccctt gttgctgtat
     6781 cccgggtaga gaggttgcag ggaggtttat gaatgttcag gttcgatgac aggggaagag
     6841 gttccaagag taacggcggc aaaaatctcc ataacggacg ctgttcgttc tgcggaaaag
     6901 ggcaggacga ggtgcagaag ctcatagcag gccccggcgt gtatatttgc gacgagtgta
     6961 tccatctgtg caatatgatc ctggtcgagg aggccgggga gactgctgcg aaagcgaaaa
     7021 ccgcagagcc cgtctttccg ctttcggagg ttccaaaacc acgagagata aaagagtatc
     7081 tggatcagta cgtcataggt caagatctgg ccaagaaagc cgtttcggtc gcagtataca
     7141 accatttcaa acggatcagc aacaacatgg cggaagagag cgatgtcgag ctgcaaaaga
     7201 gcaacgtcct gctgatgggg cctaccgggt gcggcaagac gctgattgcg cagtccttgg
     7261 cgaagaagct caacgttccc ttcgctatgg ccgatgcgac gacgctcaca gaggccggtt
     7321 atgtcggcga ggatgtcgag aatatcctcg taaaactgct tcaggccgcg gactacgatg
     7381 taaaggccgc gcagagaggc atcatctaca ttgacgagat cgacaagatc accagaaagt
     7441 cggagtcagc ctcaataaca agagacgtct ccggcgaagg tgtgcagcaa gcgctcttga
     7501 agatactcga aggcacgctt tcaaatgttc ccccaaaggg cggaagaaaa cacccgtacc
     7561 aggattttat ccagattgac acgtcgaata ttctctttat ttgcggcggg gcattcgaag
     7621 gtatcgagga aatcattggg cgcagagtga acaagaagat catcggtttt ggcggcgaca
     7681 tcatggggaa gcacgatgcc cgacgtttta atttgatgaa gcagattcag cccgaggacc
     7741 ttatggcgtt cggcttcata ccggagatcg cggggcggct tccgctgctc gttccccttg
     7801 aagagctgaa tacggatgcg ctgatccgta ttcttgttga gccgaagaac tctattgttc
     7861 gtcagtatca gaagaccttc tccatggaag gggtgtcctt ggagttcacc gaggatgctc
     7921 ttcgaagcat cgccttaaaa gccggggaga aaaacacagg cgccagaggg cttcgcgcaa
     7981 tcctcgagaa cctgatgatg gacctgatgt acgatatccc ctccagggga aaggacgtcg
     8041 gcaaggtgat cataaccaaa gaggccgtcg aaggatcgca gccccctctg gaagaagcga
     8101 gaagctcgga ggaggttgcc tgatatcatg aagaaagaag aagaagcgca ggcgcaggat
     8161 atacaggata taacggaggt ttccgcttcc ggtccgcgca gctgttttgt ccttccggta
     8221 cgggatatcg ttatcttccc cggtatcatc gcgccgcttt tcgtggggcg tccccgttcc
     8281 ctgaaggcaa tcgagatggc catgctccag gacaggaatg tcctggtagt cgctcaaaaa
     8341 gagatgcagg ttgacgaccc tagggtagag gatctctatt cagtgggcac tgtatgcagc
     8401 attctccaga tggtcaggat tcctgacgga acgacgaaag tgctcatcga gggcgtggag
     8461 cgcgttcagg ttgtttcgta cggtaaggga aaagagactc tggaggctga tgttgtatcc
     8521 ctgccgtggg aagagcacca ctccccgaac ctggaagctt tgaagaggag cgttctggaa
     8581 cagtttgaga aatacgtgac gctccacccg cggattccgg gcgaagtgct catttcagtg
     8641 atgaacgtag aggatccccg gcagatttcc gatttggtcg gctcgcacct ctcgctgaag
     8701 gtggaaaaga aacagaaact gctcgagatg gtcaatcttg aaaagagtct gaagtttctc
     8761 ctgaaggtcc tcctggaaga gatcgatatc ctacaactgg agcacgctat ccaggacaag
     8821 gtgcgccagg aagttgagcg ggggcataag gagtactacc tcagggagca gctgaaagta
     8881 atccaggacg aactcggaca gggtgaaaaa ccttccgaga tcgatgagtt gcggacgcgc
     8941 atagaggaag cggagatgcc ggaagcctct ttgagcaaag cgcttcacga gttggaccgg
     9001 ctttcaaaga tgccgctcat gtccgcagag gcaaccgttg tacggacgta catcgactgg
     9061 cttgtgaccc tcccgtggaa tacgatgacg ccggacaacc tggagataaa aagagcgcag
     9121 aaagttcttg acgaagacca ctacggcctg gagaaagtga aggagcgtat cctggaattc
     9181 ctggctgtcc gcaagctcgc gctggacaag atgaaggggc aggtactctg ctttgtcggc
     9241 cctccgggag tgggaaagac ctcgctcgga aaatcgatcg caaaggcgct cgaacgcaaa
     9301 tttgtcaata tgtcgcttgg cggtatgcgg gatgaagccg agattcgcgg ccacagaaga
     9361 acctatgtcg gcgcgatgcc gggccgcata ttgcagaaga tacgacaggc aggcgcacgg
     9421 aacccggtgc ttttgatgga cgagatcgac aagctcggac ttgacttcag gggagatcca
     9481 gcagcggcgc ttctggaagt actcgatccg gaacagaaca gctcctttac cgaccacttc
     9541 atggaggtgc cgttcgatct gagcgacgtc atcttcatca cgacggccaa tgttacccac
     9601 tctatcccca gacctctgct cgacagaatg gaggtcatca aaattccggg gtacgtatgg
     9661 caggagaaga taaagatagc gaaaaaccat atcttcccga gaattcttga agagcacggg
     9721 cttactccga agcagttgaa gataacccca agcggtctcg aacatgtcat cagcgaatac
     9781 acccgggaag cgggagtccg cggcctggaa cgagagctct ccaccatctg ccgaaaggtg
     9841 gccaggagga tcgtcgagga cgagaagaat gccgaagagc cggtttcaat aggccccaaa
     9901 cacctgaagg agtacctcgg cccaccgagg ctgtacgatc cccgtcttcc gaagacgaag
     9961 gagatcggca ccgtcgtggg gttggcatgg acggaaaccg gcggcgatgt gctcgtcatt
    10021 gaggccgtca cgatgaaggg aaaaggagaa gtcaccctta cagggaatct tggctccatc
    10081 atgcaggaat ccgcgcagac cgcgataggt tttctccgtt cgaatgcgga tggttttggc
    10141 atcggaaata tcgactggaa ggctatcgac gtccatcttc atgtcccgga aggcgctatt
    10201 cccaaagacg gcccctccgc aggaatcacc atggcggtag ccattctttc cgcggttcgc
    10261 aagagctcgg tgcttccaaa tattgccatg acaggcgagg tatctcttca gggaaaggtg
    10321 ctccccgtcg ggggtatccg ggacaagata ttggctgcta aacgacaggg gatcaagaac
    10381 atcattcttc ctctggcaaa caagcctgat gttgaagaaa tacctgagtg gtcgcgggat
    10441 ggtctttctt tccgttatgt tgaccgggtg gagaacgtgt ttgagtatgc ccttgaaagg
    10501 gctccctctc catgaactcg aagtggaaag tttcgctcga gtgcaccgcg tttacggcag
    10561 accagtttcc tcctccggag attccggaga tcgcggtggc cggacgatcg aacgtaggga
    10621 agtcgacgct tatcaacaac ctgctcgggg cccggttggc ccatgtgggg gcgactcccg
    10681 ggaagacccg gagcgtcaat ttctttcttg ttgagcggga aggagtcccg ttcaggctgg
    10741 tagatcttcc gggatttgga tatgcctccc ggagcaaagc ggagcggaac acgtggtccc
    10801 gccttatcga atcgtatgtt gccagccgga agagtctttg gcttgtgctg catcttgtcg
    10861 atttccggca cggtctcctg gaaaatgatg catcgcttca ggagtggctc gatggcatag
    10921 gccttcccgt gttcgttgtc tttacaaaag gggacaaaat agcgaaagga aagcacctga
    10981 gtacgcgaga ccagttcatc cgtaagggga tgaaatcgat cgacgtcccc atcatcacct
    11041 caggcgataa ggggactggc attcaggagc ttagggtatt tatcgagtca tttcttgacc
    11101 gtcaggagta acagatgttt cccccgcagg gggattcta
//
LOCUS       DAOPKU010000024        11083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_6188_length_11083_cov_4.909775, whole genome shotgun sequence.
ACCESSION   DAOPKU010000024 DAOPKU010000000
VERSION     DAOPKU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11083
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6188_length_11083_cov_4.909775"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /gene="dnaX"
                     /locus_tag="PK849_01450"
     CDS             complement(<1..1197)
                     /gene="dnaX"
                     /locus_tag="PK849_01450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPE64818.1"
                     /translation="MSVSLYRRYRPRSFDEVAGQETVVEVLKKALQRDQVAHAWLFSG
                     PRGCGKTSAARLLAKALNCTQLKDGYEPCGECLQCVSIGAGDSLDVVEIDGASNNGVE
                     EIRELKTHVSLSPFSAKWKVYIIDEVHMLSISAFNALLKTLEEPPPFVVFILATTEPH
                     KVPVTIRSRCQHIPFHRIELRAICSRLEAVAEKEGVPADPRALREIARHADGALRDAL
                     SLMEQALSLGNGEVSMEAADRLLGGGTLSDLERWIASLKENNVQPFLFLEEMFQKGAS
                     PQRVVEGVFLLFRNLWAVKRWGRPVLEALSLAGSEMEFLCEEAASWKESQLSEMMLFC
                     SKLIPQVRAGLRSDVLSGLLAAKGLECRSFAEGPRADVKEEAPALRPEPPVVSPARRA
                     QRVEDVT"
     gene            complement(1355..2452)
                     /locus_tag="PK849_01455"
     CDS             complement(1355..2452)
                     /locus_tag="PK849_01455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPE64819.1"
                     /translation="MRGSLAMKFNAVRKLLFPVCCLLLLAASSLFADELTKEIALGKK
                     VAAEVEEKWELVTDPARTARLGMLLTRLLPHTTRPLPFEARIVREDMINAFSLPGGIV
                     YFTTGMLDFLRTDAEIAAILAHELVHADRRHVMIQTARSSKISIAALALMIATQGAAG
                     PMILTSLAQVAVTNSYSKDLEREADKEGFRMLVAAGFPPAAMVTSLEAMIFDQLKHPY
                     VDPGVFMTHPELSERVEYILKMSEEMKVPIRRKHALNLLRPAVVESGDHLALTLDGTE
                     VWRSNRTDEGAALLRKAVETIDAFLQMETPPYEIQVLDLDGKGALRIGPALVAREPLP
                     GGTPPLAELRVSLVQALGAALDKHRSANYHR"
     gene            complement(2449..2904)
                     /gene="flgN"
                     /locus_tag="PK849_01460"
     CDS             complement(2449..2904)
                     /gene="flgN"
                     /locus_tag="PK849_01460"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HPE64820.1"
                     /translation="MQADLEKKLSSLVSSELALFNELALLVEKEEECVLQEDMECLLN
                     VLQEKQDVISRQEKIHEGWSFISSSLGISEGRDGPSFWSRLGELLGEGADDLKASLSV
                     IRDVAGNVLEQEIRVQGLLEKHVDSLRSQMAQVSRGKKALQGYSKAGGV"
     gene            complement(2894..3382)
                     /gene="fliS"
                     /locus_tag="PK849_01465"
     CDS             complement(2894..3382)
                     /gene="fliS"
                     /locus_tag="PK849_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPE64821.1"
                     /translation="MDSKNAQNAQTVYLANKVNTASREQLLLITYDIGIKSCKGAENA
                     LDAGVFDEANKHIQKAQDVLRELMVTLRVEEGGEVAQNLMRLYDFMYLMLVEANVSKD
                     REKISVVRGMLEELRTTWEEAIVKLAEEAGIEKPMPHLQGGAAAKEEARAVPVGGLNI
                     AG"
     gene            complement(3558..4745)
                     /gene="trxB"
                     /locus_tag="PK849_01470"
     CDS             complement(3558..4745)
                     /gene="trxB"
                     /locus_tag="PK849_01470"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPE64822.1"
                     /translation="MEKRELVIIGAGPAGLSAAIYGRRAGLDVLLLEKGVPGGQINIT
                     EEIENWPGVEHATGPELGRMFRSHAEKFKTEFRDVDVSKVEIRGDAKIVVTNKGEIEA
                     EAVIIATGAYFRRLGCEGEAEHIGQGVSYCAVCDGAFFEGEEIAVVGGGNTAVEEGVY
                     LTNFASKVFIIHRRDEFRADRAAVEHAMSNPKIVPVWNTVVEKIEGDGMVENLVLKNL
                     KTGEVSNLPVAGVFMFVGQSPHDECIRGLVDASKGGWIKTNDAMETSVEGVFAAGDVR
                     DKGLRQVITAASDGAIAAMSASSYINEQVHLRSVLIEPAHVVALFYSSIDREQVKLSD
                     EVEALSQKSGKKVALIDGYKNHRMVEKFGLGSLPAMVELSSGTVTRKASVAAASDAAS
                     FLK"
     gene            complement(4791..5636)
                     /gene="prmC"
                     /locus_tag="PK849_01475"
     CDS             complement(4791..5636)
                     /gene="prmC"
                     /locus_tag="PK849_01475"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPE64823.1"
                     /translation="MTAAEARRRLVRELSAAGVFSPGLEADVILSVACGVSSALLRAR
                     PDFGLTEENVSELARITGRRARREPLQYILGSWEFYGRALETPPGVLIPRPETEVLVE
                     KALERLPPEGGVFLDWGTGSGCIALSLLCENASACAIAADCNPRALRVSWTNMKRWGV
                     LRRCLLWHSRTPDDLPVSPASLDMLVSNPPYIPTDRMNTLMEEVRYEPQSALDGGIDG
                     ADCYRRLFIAGERMLRRGGSLLFEAGDGEQIAFLAGIAPECFELDGIFEDFSKSPRVI
                     AWRRV"
     gene            complement(5638..6714)
                     /gene="prfA"
                     /locus_tag="PK849_01480"
     CDS             complement(5638..6714)
                     /gene="prfA"
                     /locus_tag="PK849_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPE64824.1"
                     /translation="MNFQDKLSELERDFEETEAKMADPAFHSNMKELQGLAKRHSKLA
                     PVVSKFREFKKVTVDLEEARSLLHCGDPEMESLAKDELSSKEDRLREIEEEMKLLLLP
                     EDPNDEKSVIVEIRAGTGGEEAALFAANLFRMYTRFAEREGWRTEIVDYNETGIGGYK
                     EIIFRIDGDGVYSKLKFESGVHRVQRVPATEAAGRIHTSAATVAVLPEVEDVDVEIRT
                     EDLKIDTYRSSGAGGQYVNMTDSAVRITHLPTGVVATCQDERSQLKNRVKAMAYLRAK
                     LYDLELQKQNDEVASERRGQIGSGDRSERIRTYNFPQNRISDHRIGMTLYKLDQYLDG
                     DLYELVDAMTMADQSQKLHAIEAN"
     gene            complement(6711..7649)
                     /locus_tag="PK849_01485"
     CDS             complement(6711..7649)
                     /locus_tag="PK849_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HPE64825.1"
                     /translation="MKRLFASMLLFLAEGLEQKRIPVGGQAVIEGVLMKGPERWGLSV
                     RKPDGEIENETWQSFSRTKRMPWKLPIVRGVVTMVEMMTVGFRALNKSAQIAVGEEEK
                     ITPKEFLLSVLVAIGAVVGLFIALPLWLSDLAVTSWGLTQTGKNILEGCVRGLVFISY
                     VGGIGLWSEIRQVFRYHGAEHKTINAFESGADMTPENIRKFSRIHPRCGTSFLLIVVA
                     VSIVVFSIAGSGSILWRIGSRIVLLPLVMGISYEIIKGASCAGILGRILMAPAMSFQY
                     LTTREPDEKQIEVALTSLETALGRKLSPVAAEAPAE"
     gene            complement(7663..8358)
                     /gene="thyX"
                     /locus_tag="PK849_01490"
     CDS             complement(7663..8358)
                     /gene="thyX"
                     /locus_tag="PK849_01490"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPE64826.1"
                     /translation="MRCGVLLLSFTPRPDAVVAAAAKLCYSDVSAADLLSDESSEKMR
                     GLLRHIRASGHFSPFEHASFTFAVDGLSRVTSHQLVRHRIASFSQQSQRYVTMGEPDV
                     VVPPSIAERPELTDGFLEMTRSAHAFYRKLVESGVPKEDARYILPHGWSTRLVMTMNA
                     RELHHFFTLRLCRRAQWEIRNLAREMLLAVREAAPLLFETAGPSCLVRGRCEEKSPCG
                     RPFASLEELLDDN"
     gene            complement(8412..8645)
                     /gene="rpmE"
                     /locus_tag="PK849_01495"
     CDS             complement(8412..8645)
                     /gene="rpmE"
                     /locus_tag="PK849_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPE64827.1"
                     /translation="MKKDIHPHYEKCTVNCACGNSFETRSTQKEIRVGVCSSCHPFFT
                     GKKGSRVLTEAGRLEKFQKKYEGINYGQKESAE"
     gene            complement(8766..9353)
                     /locus_tag="PK849_01500"
     CDS             complement(8766..9353)
                     /locus_tag="PK849_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HPE64828.1"
                     /translation="MIWKNIFKNPQDEDIEEEPVEMELLQGMEEEREPVTLQMNGPSA
                     DSERSSIELNLKIRIDDENQAGRAALLLASTTTSEAENIFKRELASLGWKSVATEVGG
                     LAGDLPQKITRALVGASLNAGVVEKKRNEMHALMHAALEALDGFLVVGMLEASVGAKI
                     AIVRNNRWISVAVMGDTAYHAVAHHERCGLGVMHI"
     gene            9596..10558
                     /locus_tag="PK849_01505"
     CDS             9596..10558
                     /locus_tag="PK849_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE64829.1"
                     /translation="MNKKFTAGLALLLMVSIFAGAASATTFISIATGGTGGTYYPLGG
                     GIADIISRNVPDFQVTSETGNASAANINLIGTHQIEMAFAQNDIAFWAAKGMSPFKEK
                     YDNIRVVASLYPEHVHCVTLKGSGVSDIMDINGKRVSVGAPGSGVLGDVSAILKVAEI
                     KFSDMQTDFLDFNNTTQRFKDGQLDVGFVVAGYPTSSLMDLATTHDIGLVSFNDEFMS
                     KLVKEFPFFIKDTIPAGTYNGVDQDTPTPAVMAMLVCDAQLSDDVVYKFTKALWDNIA
                     DLHKVHAKAKLITLETALDGVSVPVHPGAAKFYTEKGMKVPAIK"
     gene            complement(10615..>11083)
                     /locus_tag="PK849_01510"
     CDS             complement(10615..>11083)
                     /locus_tag="PK849_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64830.1"
                     /translation="SQVSRRMVRSVGCPLSRLEDVFSGLRDENAVLGKTLEKAAQLVA
                     IPWDCSETGGVSLYTAVIPGVPKELVTAAARRWSDAHPGGIALLLLPDPEKEGGAFLL
                     YRGAAVERDFSRFLKSSPSLEARGGGRSDWLNGASSCMDPGVWTQEIERYMSE"
BASE COUNT         2348 a         3407 c         2993 g         2335 t
ORIGIN      
        1 agtaacgtcc tcgacccttt gcgcccggcg agcaggagac acaacgggag gttcaggacg
       61 aagcgcaggc gcctcctctt tcacgtccgc gcggggccct tccgcaaagc tgcggcattc
      121 aagtcctttc gcggccagca ggccggagag gacgtccgag cgaagccctg cgcgcacctg
      181 cgggatcagt ttggaacaga agagcatcat ctcggagagc tgcgattcct tccaggacgc
      241 agcctcttcg cacagaaact ccatctcgct cccggcgagt gaaagcgcct caagaaccgg
      301 cctgccccat cgtttcaccg cccagaggtt gcggaagaga agaaagacgc cctcgacaac
      361 tctctgcgga gatgctcctt tctggaacat ctcctccagg aagagaaacg gttggacatt
      421 gttctccttc agggaggcga tccaccgttc gaggtcggag agcgtccctc cgccgagcag
      481 acgatcggcg gcttccatgg agacctcgcc gttaccgagc gagagcgcct gctccatcaa
      541 ggagagcgcg tcccgcaaag ctccgtcggc gtgccgcgcg atctcgcgca acgccctcgg
      601 atcggcagga accccttctt tctccgcgac cgcctcaagc ctggagcaga tggcccgaag
      661 ctctatcctg tggaagggga tgtgctggca ccgggagcgt attgtcaccg gcaccttgtg
      721 cggttccgtg gtcgcgagaa tgaagacgac aaagggaggc ggctcctcga gtgttttcag
      781 cagcgcgttg aaagccgaga tggagagcat gtgcacttcg tcgatgatgt acaccttcca
      841 ttttgcggaa aacggagaga gggagacgtg cgtcttgagc tcgcggatct cctcaacgcc
      901 gttgttggaa gctccgtcga tctcgactac gtcaagactg tcccccgcgc ctatggagac
      961 gcactgtaga cactcgccgc acggctcgta accgtctttg agctgcgtac agttaagagc
     1021 cttggcaagg aggcgcgccg cggaggtttt tccgcagccg cgcggcccgg agaagagcca
     1081 ggcgtgggca acctgatctc gttgaagcgc cttcttcaac acttcgacga cggtctcctg
     1141 gccggcgact tcgtcgaacg accggggcct gtaccggcgg tagagagata cggacacgga
     1201 gtactcccct ttcaaagggt cgagtcaaag ggtcgagcct gatcgaccgg gactgcatgt
     1261 cagtaaggag caaactcctc ctgatcgaaa aatgcacaaa aaaaaggtgc gggggccggc
     1321 attttctccc ccgcacctat tgtaccatca gaaatcagcg atggtagttt gcgcttcgat
     1381 gtttgtccag ggcggctccc agagcctgca caagggagac gcggagctcg gcgagaggag
     1441 gcgttcctcc cgggagaggt tcccgggcca ccagggctgg gccgatacga agcgctcctt
     1501 tgccgtcgag atcgaggacc tgtatctcgt agggcggcgt ctccatctgg agaaaagcgt
     1561 cgatcgtctc aaccgctttc cgaagaagcg cggccccttc gtctgtcctg ttcgaacgcc
     1621 agacctccgt tccgtcaagg gtcagcgcaa ggtgatcccc ggattcaacc actgctggac
     1681 gtaaaaggtt caacgcatgt tttctccgga tcggaacctt catctcttcc gacatcttca
     1741 gaatatactc aaccctctcc gaaagctcgg ggtgggtcat aaatacaccg ggatccacgt
     1801 acggatgctt gagctggtcg aagatcatgg cctcaagcga ggtgaccatc gctgcgggag
     1861 gaaagcccgc ggcgacgagc atacggaacc cctctttgtc ggcctcccgt tccaggtcct
     1921 tgctgtagga gtttgtcacc gcaacctgcg caaggctggt caggatcatc ggtcctgcgg
     1981 cgccttgggt ggcgatcatc aacgccaaag cggcgataga aatcttcgac gaacgggcgg
     2041 tctggatcat cacgtgccgc ctgtccgcat ggacgagctc atgagccagg attgccgcaa
     2101 tttccgcgtc tgtccgcaaa aagtccagca tccccgtggt gaaatagaca atccctccgg
     2161 gaaggctgaa cgcattgatc atatcctccc gtacaatacg agcttcgaag ggaagaggtc
     2221 tcgtcgtgtg cggaagcaac cgggtgagga gcattccgag acgcgcggtc cgcgcgggat
     2281 ccgttacaag ctcccatttt tcttcaactt ccgccgcgac tttcttcccg agagcaatct
     2341 cttttgtcaa ttcgtcggca aaaagagacg aagctgccag aagaagcagg caacagaccg
     2401 gaaagagcag tttgcgaaca gcattgaatt tcatggcaag cgaacctctc atactcctcc
     2461 tgcttttgaa tagccttgca gcgctttttt cccccggctc acctgggcca tctggcttcg
     2521 gagggaatcg acatgctttt ccagaagccc ctggacgcgg atttcctgtt ccagaacatt
     2581 tccggcgaca tcccggataa cagaaagaga ggctttcaaa tcatccgcgc cctcgccgag
     2641 aagttctccc aggcggctcc agaatgaagg gccgtctctc ccttcggaga tgccaagcga
     2701 cgaagagatg aagctccacc cctcgtggat tttttcctga cgggaaataa cgtcctgctt
     2761 ttcctgcagc acgttcaaaa gacattccat atcctcctgg agaacgcact cctcttcttt
     2821 ctccacaaga agagcgagct cgttgaagag cgcgagctcg gaggaaacaa ggctggagag
     2881 ttttttttcg agatcagcct gcaatattca accctccaac cgggacagcg cgagcctcct
     2941 ctttcgcagc ggcgcctccc tgaagatgag gcataggctt ctcaataccc gcctcttccg
     3001 caagcttcac aatagcttcc tcccatgtgg tccgaagctc ttcgagcatt cctctgacga
     3061 ctgagatctt ttcgcggtcc ttgctcacgt ttgcctcgac aagcatgaga tacatgaagt
     3121 cgtaaaggcg catcagattc tgcgcaactt cgccgccctc ttcgacacgg agcgtcacca
     3181 taagctcacg aagaacgtcc tgagcttttt gaatatgttt attcgcctcg tcgaaaacac
     3241 cggcatccaa ggcgttctca gctcccttgc agctcttgat gccgatatcg tatgttatga
     3301 gaagaagctg ctcgcgagag gctgtgttca ctttattggc aaggtagact gtctgggcgt
     3361 tctgtgcgtt tttcgaatcc attgcggaga tcactccttt ctctccatct tgtttcggta
     3421 aaggagagag aaaaccttac caggaacagc ggtcattttg aaagtacgct ccgaatcttt
     3481 cctgcccgac tccaggccgg gcgaagaaaa agagggagcc gcatcctgct gcatgacacg
     3541 accccctttg agaaaaacta ctttaaaaaa gatgcggcat ccgaagcggc ggcaacggaa
     3601 gccttcctgg tcacagtgcc ggaggagagt tcaaccattg caggaagaga accgagaccg
     3661 aacttttcca ccatccggtg gtttttgtac ccgtcgatga gggcaacctt cttcccgctc
     3721 ttctgcgaga gcgcctcgac ctcgtcggag agcttgacct gctccctgtc gatgctggag
     3781 tagaagagcg cgaccacatg cgcgggctcg atgaggaccg agcgcaagtg aacctgttcg
     3841 ttgatatacg aggaagcgct cattgccgcg attgcgccgt cggaggccgc tgtaatcacc
     3901 tggcgaagcc ccttgtcccg gacgtctcca gccgcgaaga cgccttcgac ggatgtttcc
     3961 atggcgtcgt tcgtcttgat ccagccgcct ttcgacgcgt caacaagccc ccggatacac
     4021 tcgtcgtggg gagactggcc gacgaacatg aagacgcccg ccacaggaag gttggagacc
     4081 tcgccggtct tgaggttctt caggaccaga ttctcgacca tgccgtcgcc ttcgatcttt
     4141 tcgacaacgg tgttccacac cgggacaatc ttcggattcg acatggcatg ttcgactgcc
     4201 gctctatcgg cgcggaactc gtctcttctg tggatgatga agactttcga agcgaagttc
     4261 gtgaggtaga cgccctcttc gacggccgtg ttgccgccgc cgacgaccgc gatctcctcg
     4321 ccttcaaaga aggcgccgtc gcagactgcg cagtagctca cgccctggcc gatatgttcg
     4381 gcttcgccct cgcacccgag cctgcggaaa taggccccgg tggcgatgat taccgcttcg
     4441 gcttcaatct cgcccttgtt tgtgacgacg atcttggcgt cgccgcgaat ttcgaccttg
     4501 gaaacgtcga catcgcggaa ttcggtcttg aacttctcgg cgtggcttct gaacatcctg
     4561 ccaagctccg gaccggtggc atgctccact ccgggccagt tctcaatttc ctcggtgatg
     4621 ttgatctgtc cgccgggcac gcccttttcg agaagaagca cgtcaagtcc ggcccttctg
     4681 ccgtagatcg cggccgataa accggccggc cctgcaccga tgattacaag ctctcttttt
     4741 tccatatcga aactatccct ccagataaga cacatgatct cttttcgaaa ttatacacga
     4801 cgccatgcaa ttacgcgagg cgacttcgaa aaatcctcaa aaattccatc caattcaaag
     4861 cactccggag cgatgcccgc taaaaaagca atctgttctc cgtcgcccgc ctcgaagaga
     4921 agactccctc cgcggcgcag catccgttct cctgcgatga aaagccgcct gtaacagtcc
     4981 gctccgtcta taccgccgtc caaggcggat tgcggttcgt accgaacctc ctccatgagc
     5041 gtgttcatac ggtccgtcgg gatatagggc gggttgctta cgagcatatc gagggatgcg
     5101 ggagagacgg gaagatcgtc gggagtgcgc gaatgccaca ggaggcacct ccggagaacg
     5161 ccccagcgct tcatgttcgt ccacgatact cgaagcgcgc gagggttgca atcggcggct
     5221 atagcgcagg cggaagcgtt ttcgcagagg agggaaagcg cgatgcaccc gctccccgtc
     5281 ccccagtcga gaaagacgcc tccctcgggt ggaaggcgct ccagggcttt ctcaacgagc
     5341 acttccgtct ccggcctcgg aatcaggaca cccggcgggg tctcaagggc gcggccgtaa
     5401 aattcccatg atccgagaat atactggaga ggctcccttc gagcgcgccg tccggtgatg
     5461 cgggcgagtt ccgaaacatt ctcttcggtc agcccgaaat cgggtcgggc acgaaggagc
     5521 gcggacgata cgccgcacgc aactgaaagg atcacatccg cttcgaggcc gggagaaaag
     5581 actcccgccg ctgaaagttc gcggacgagc cttctcctgg cctcggctgc ggtcatgcta
     5641 gttcgcctcg atagcgtgga gtttctgcga ctggtcggcc attgtcatgg cgtcgacgag
     5701 ttcgtaaaga tcgccgtcca gatactggtc cagcttgtag agggtcatcc cgatgcgatg
     5761 atcggagatt ctgttctgag ggaaattgta ggtccgaata cgctccgaac ggtcccccga
     5821 gcctatctgc cccctgcgtt cggaggctac ttcatcgttt tgcttctgca gctcgaggtc
     5881 gtacagcttg gcgcgaaggt aggccatcgc ctttaccctg ttcttcagct gtgagcgttc
     5941 gtcctggcag gtggccacga cgcccgtcgg gaggtgcgta atccttactg cggagtccgt
     6001 catgttgacg tactgcccgc ccgccccgct cgaacggtag gtgtctattt tcagatcctc
     6061 ggtccggatc tcaacgtcga catcctccac ctcgggaagc accgcgactg tcgccgcgga
     6121 ggtatgtatc cttccggcgg cctctgtcgc gggaacgcgc tgcaccctgt gcacgccgct
     6181 ctcgaacttg agcttgctgt acacgccgtc gccgtcgatc cggaagataa tctccttgta
     6241 tccgccgata ccggtctcgt tgtagtcgac gatctcggtt ctccacccct cgcgctccgc
     6301 gaagcgtgtg tacatccgga agaggttcgc ggcaaagagc gcggcctcct ccccccccgt
     6361 cccggcgcgg atttccacaa taacgctctt ttcgtcgttc gggtcctcgg gcagaagaag
     6421 gagtttcatc tcctcttcga tctcccgaag acgatcttct tttgaggaga gttcatcctt
     6481 ggcgagcgac tccatctccg gatcgccgca gtgaagcagc gaccgcgcct cttcgaggtc
     6541 aacggttact tttttaaact cgcggaactt ggagacaacg ggagcgagct tcgaatggcg
     6601 cttcgccagt ccttgcagct ccttcatatt cgagtgaaag gcagggtcgg ccatcttggc
     6661 ttctgtttct tcaaaatccc tttcgagctc gctcagcttg tcttggaagt tcattcagca
     6721 ggcgcctccg ccgcaacagg cgagagttta cggccaagcg ccgtctcaag cgacgtcagg
     6781 gcgacttcga tctgcttctc gtccggctcc cgcgtcgtga gatactggaa ggacattgcc
     6841 ggagccatga ggatcctgcc cagaataccg gcgcaagagg ctcctttgat gatctcgtag
     6901 gaaattccca tgaccagcgg aagaagcacg atcctgctgc cgattctcca gaggatggag
     6961 ccgcttcccg cgatggagaa gaccacgatg ctcaccgcaa cgacgatgag gaggaacgat
     7021 gtgccgcacc gcggatgaat ccgcgaaaat ttccgtatgt tctcgggcgt catgtcggcg
     7081 ccggattcaa aggcgttgat cgtcttgtgc tccgccccat ggtagcggaa gacctgacgt
     7141 atctcactcc agaggccgat gccccctacg taggaaataa agacaaggcc cctgacacat
     7201 ccttcgagaa tgttttttcc ggtttgcgtc agcccccacg aagtgacggc aagatcggag
     7261 agccaaagcg gcaaggcgat gaacagtccg acgaccgccc caatggcgac aaggaccgag
     7321 aggagaaact ccttcggcgt tattttttct tcttcaccga cggcgatctg cgcggacttg
     7381 ttcagcgcgc ggaaaccgac cgtcatcatc tccaccatcg tcacgacgcc gcgcactatc
     7441 ggaagcttcc acggcatccg cttcgtccgc gagaagcttt gccacgtctc gttctcgatc
     7501 tctccgtccg gttttctgac cgaaagtccc cagcgctccg gacccttcat aagaacgcct
     7561 tcgatgaccg cctggccgcc gaccggtatt ctcttctgct caagcccttc cgcaaggaag
     7621 aggagcatcg acgcaaataa tctcttcatg ttcacctcac acttaattat catccaaaag
     7681 ttcctcaaga gacgcaaacg ggcggccgca cggagacttt tcctcgcagc gtccgcgaac
     7741 caggcacgac ggccccgccg tctcgaagag caggggcgcc gcctcacgca ccgcgagaag
     7801 catctcccgc gccagattcc gaatctccca ctgcgccctg cggcagagcc ggagtgtgaa
     7861 aaaatgatgg agctcccgcg cgttcatcgt catgacaagc cgtgtgctcc agccatgggg
     7921 caaaatgtag cgggcatcct ccttcggaac gccgctttcg acgagcttcc ggtagaaagc
     7981 gtgcgccgat ctggtcattt cgagaaaacc gtccgtgagt tcggggcgtt ccgcaatgga
     8041 agggggtaca acgacatcgg gctctcccat cgtcacgtac cgttggctct gctggctaaa
     8101 ggaagctatt ctgtggcgga caagctgatg agacgttacc ctgctcaagc cgtccacggc
     8161 aaaggtgaag gaggcgtgct cgaacgggga gaaatgaccg cttgcgcgta tatgacgcaa
     8221 cagaccgcgc atcttctccg aagattcatc cgagagaagg tccgcagccg aaacgtcgct
     8281 gtaacagagt tttgccgcag cggcgacaac agcatcaggc cggggcgtga aggagagaag
     8341 gaggacgccg cagcgcatac agcccacttc ctttccgaaa aagaggggac cgaagccccc
     8401 tcgtcgttgt cctattccgc gctttccttc tggccgtagt tgattccctc gtacttcttc
     8461 tggaatttct cgagtcttcc ggcctccgtg agcacacggc tgcccttctt cccggtgaag
     8521 aaggggtggc aggaggaaca aacgccgacg cgaatctcct tctgcgtcga acgggtctcg
     8581 aaagaatttc cgcatgcaca gttcaccgtg cacttctcgt agtgaggatg aatatctttt
     8641 ttcatctatg ggcacctccg aaattatcgt aaagccttgc atatatctcc aaacgaagag
     8701 aaactttagc acaggaaggc cccggacgca agagcaaatt ttcttccggg accgattttc
     8761 tcccttcaga tatgcatgac tccgaggccg cagcgttcat gatgcgcgac ggcatggtag
     8821 gccgtatctc ccatgacggc gacggagatc cagcgattgt tccggacgat ggcgatcttc
     8881 gcgccgacgc tcgcttcgag catgcctacg acgagaaaac cgtccagcgc ttccagcgcg
     8941 gcgtgcatca gcgcatgcat ctcgttcctt tttttctcca ccacacccgc gttgagcgaa
     9001 gccccgacaa gcgcccttgt gatcttctga ggaaggtctc cagcaagtcc cccgacttcc
     9061 gtcgccaccg atttccaacc cagggaagcc agctctctct tgaaaatgtt ctccgcctcg
     9121 ctggtcgtcg tcgatgcgag gagaagggcg gctctccccg cctgattttc gtcgtcgata
     9181 cgtatcttga gattgagttc gatcgagctt ctctccgaat cagcgctcgg cccgttcatc
     9241 tgcagcgtaa cgggctccct ctcctcctcc atcccttgca aaagctccat ctcgacgggt
     9301 tcctcctcaa tgtcttcgtc ctggggattc ttgaagatat tcttccagat cacaacatac
     9361 agctcctctc tggtgagttc tgaatcccat tataaaggaa tttgaggcaa gagtcacttt
     9421 tccttccaga gattcaataa aaaaacacaa gagtttcttg ataccccggg gaattcgtcc
     9481 tatactatag gaggcaaaga gaacgctcaa ttcaaataca aaggggggaa caaaatacac
     9541 gcgagttttt gaatcattca aagcaccaca aattcaagct aggaggaata cacatatgaa
     9601 taagaaattc accgcaggtc ttgctcttct tcttatggtc tccattttcg cgggcgcggc
     9661 gtccgcaacg accttcattt ccatcgccac gggcggcacg ggcggcacct actatcccct
     9721 cggcggcggc attgccgaca tcatcagcag aaatgtcccc gacttccagg tcacttccga
     9781 aaccggcaac gcttccgcag ccaacatcaa ccttatcggc acccatcaga tcgagatggc
     9841 ttttgcccag aacgacatcg ccttctgggc agcaaaggga atgtctccgt tcaaggagaa
     9901 gtatgacaat atccgcgttg tggcctccct gtaccctgag cacgtccact gcgtcaccct
     9961 taaggggagc ggcgtgagcg acatcatgga cataaacggc aaaagagtct ccgtcggcgc
    10021 tcccggctcg ggcgtgctcg gcgacgtatc cgcgattctg aaggtggcgg agatcaaatt
    10081 cagcgatatg cagaccgatt tcctcgactt caacaacacc acccagcgct tcaaggatgg
    10141 gcagctcgac gtcggtttcg tcgtcgccgg ctaccccaca tcctccctca tggacctggc
    10201 gaccactcat gacatcggcc tcgtcagctt taacgacgag ttcatgagca agctcgtcaa
    10261 ggagttcccc ttcttcatca aggacaccat ccccgccggc acctataacg gcgtcgatca
    10321 ggacactccg actcccgccg tcatggcgat gctcgtctgc gacgcgcagc tttccgacga
    10381 cgtcgtctac aagttcacca aggctctctg ggacaacatc gccgacctgc acaaggtcca
    10441 cgcgaaggcg aagctgatca ccctcgaaac agcgctcgac ggcgtctccg tccccgtaca
    10501 tccgggcgcg gcgaagttct acaccgagaa gggcatgaag gttcccgcta tcaagtagcg
    10561 acgcctttcc caagttctca atgaaagaag gcggctctcc ggccgccttc ttttttattc
    10621 cgacatatac cgttctattt cctgcgtcca cactcccgga tccatacagg acgaagcgcc
    10681 gttcagccag tcgcttcgcc cccctcctct ggcttcaaga gagggcgacg atttcaggaa
    10741 gcgagaaaag tcacgctcta ccgcagcgcc tctgtagagg agaaacgctc ccccctcttt
    10801 ctccgggtcg gggagcaaca gaagcgcgat ccccccgggg tgggcgtcgg accatctgcg
    10861 cgccgcagcc gtcacgagct ccttcggaac tccgggaatg accgcggtat aaagggaaac
    10921 tccgcccgtt tccgagcaat cccagggtat ggcaaccaac tgcgcagcct tctccagagt
    10981 cttgccgagc acggcgttct cgtcccgcag tccggagaag acgtcctcaa gccgcgaaag
    11041 cggacacccc acgctccgta ccattcggcg gctcacttgg ctg
//
LOCUS       DAOPKU010000025        10513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_6637_length_10513_cov_5.040448, whole genome shotgun sequence.
ACCESSION   DAOPKU010000025 DAOPKU010000000
VERSION     DAOPKU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6637_length_10513_cov_5.040448"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PK849_01515"
     CDS             <1..283
                     /locus_tag="PK849_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPE64831.1"
                     /translation="IYYATGSRRKAFNYSFLSGVSEPIGALLAWLVLAPFLSGALFGV
                     IFASVAGIMVFISVDQLLPAARKYGEHHVSIYGLVIGMMIMAVSLLLFI"
     gene            297..1427
                     /locus_tag="PK849_01520"
     CDS             297..1427
                     /locus_tag="PK849_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPE64832.1"
                     /translation="MGITLNVEQYLKDLEYLVNIDSQSKDPEGVAKVAAFFEKAFGDI
                     GWTVERVVFDPTAGPSLKITNGEPPYDALLLGHLDTVFPKGTVAERPFSRDEKRAYGP
                     GVNDMKSGLLFGLYAARALTAKGSKGSFCIAYNSEEEIGSRRARPWIEQLAQESRTSV
                     ILEPARPNGNLMNARKGLGRIEVTFNGKAAHAGVEPEKGVSAVNEMAYWIIALHGLTD
                     FEKGTTLNAGIVAGGTGANVVPEKATMTVDVRVKIPEEAGRIAEKILQLKAHPATPGI
                     SVDADFILTRPPMNPGEKTPKLCALVEEAGKEAGVEFGWQSTGGGSDGNFTAALGVPT
                     VDGLGAMGGGSHSIGEYIEIDALPSRFAMLLGLLERIPDAGF"
     gene            1431..2642
                     /gene="fdhD"
                     /locus_tag="PK849_01525"
     CDS             1431..2642
                     /gene="fdhD"
                     /locus_tag="PK849_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPE64833.1"
                     /translation="MNMKGIILAVCTAPAKGLQKHDQGEGRLVPGKGIDGDGHFGFAH
                     RQVSLLESEEIEKMKKVIPTLFHGAFAENLVTEGIDLKRLILGDTLRIGDALLRVTQI
                     GKECHSHCAIYEAAGDCIMPRLGIFCEVLSEGTVTVGDTIEFVHEDAPQVLVPRTITR
                     LDGTKRETVTDRLLKEGRVTLVLDGEEEVTAVCTPGEERYWAVGHLKCRRLLKSREDI
                     ASLEITPGAVSVKRKQMTEGLPLLQRILSSSASALLAEGAEEQLGGVIPSEWRIPASV
                     LREGAEWLSEAPIFRATGGTHVAALMARDGEKLFRTEDIGRHNAVDKAVGWAVLHDVP
                     LGESALLVSGRLPVDMVMKALGAGIPVLGSVSAAIAEGAEAAEKGGLTLAGFIRGERM
                     NIYTHPERISD"
     gene            2770..3800
                     /locus_tag="PK849_01530"
                     /pseudo
     CDS             2770..3800
                     /locus_tag="PK849_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(3970..4929)
                     /locus_tag="PK849_01535"
     CDS             complement(3970..4929)
                     /locus_tag="PK849_01535"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPE64834.1"
                     /translation="MNDILQRIGELGIVPVVKLDKASDALPLGKALLEGELPIAEVTF
                     RTDAAEESIRIMSRELPELLVGAGTVLTRDQVKKAVDAGAKFIVTPGFNPSVVDYCVE
                     HNIPITPGINSPSQIEMALERGLDVVKFFPAEQSGGLPMLKAMSAPYGSVRFIPTGGV
                     DAKNMPSYLAFNKILAVGGSWMVKPEVVAEGKFDEIARMTREAVFAMHGFELIHLGIN
                     CPDEKEALAGAERLAALFGMAVKDGNSSAFVGKGFEFMKSMYLGEKGHVAVGCNDVMR
                     AAAFLKRKGIASLPETEKKKDGRTVALYLDLHIGGFIFHLLQK"
     gene            complement(5134..5598)
                     /locus_tag="PK849_01540"
     CDS             complement(5134..5598)
                     /locus_tag="PK849_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPE64835.1"
                     /translation="MAEQQLVVFALGKEEFGIDISRVREIVRLQNITTIPQSMDFVEG
                     IVNLRGQIVPIVDLCKRFLVADRSGSSDTERRIIVVNMSGQNIGILVDGVSEILRIPD
                     ESIEPTPPIVASGVSSDFIRGVAKVDGRLIIFLDLDRIFSVEEQEALYQSVE"
     gene            complement(5722..7767)
                     /locus_tag="PK849_01545"
     CDS             complement(5722..7767)
                     /locus_tag="PK849_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE64836.1"
                     /translation="MTIRNKLLVMALVVLAMIGTMAGVTYYRAGSIIEGLVDTAGVEI
                     VKSAAENVDARLNKVEAIVLTAAETVRDAMLRLNVTDEDGVEKIVAALTARTKNEGIM
                     DLYMGHEATGKFSDGTGWKEPDDYDCRTRSWYKEAVAAGKGKIIFTEPYVDGITKQVV
                     ISTATAIYDDAGKLLGVVGGDMDISGLSDYVVGLQIFGKGSGLMLLKSGIFVAYNEKD
                     DVLKANMMTDEKFAEPIRNIARKMVSGETGYESYVYQGEERQMFFAPTQRAFYLGILF
                     PAAEINAMVNALTFILLGLAVVAVLVTGGTIFFIARGLTKSIRSMESVTEKLGEGDLT
                     VSYEASGKDEIAHISGILNGMVFSLREVMGSIRNESQETAARAETLASLSEETLASME
                     EVSSSIEKVQDMMEHSSSALQETNASIEEIAAGAQTAARATNEGADGATNASDAANAS
                     LQEVGRAINNIRNAGSESAKSIDRIKELGKSVEAISGFVTTITSIADQTNLLALNAAI
                     EAARAGEAGRGFAVVAEEVRKLAEESARAAHEVSKLINELQKHSDDSIAATEKTGTIL
                     DDTMKGAEEMRQKLKGAVDAIVKIAEAVQNIAAVSEEQAASSEEMTSAIQSVTESTGQ
                     VVSSVGSIRGASHETTKAAESIATEAQAMAVTSEKLQTLVSRFIVDDARRSGLALKK"
     gene            complement(8210..10237)
                     /locus_tag="PK849_01550"
     CDS             complement(8210..10237)
                     /locus_tag="PK849_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE64837.1"
                     /translation="MNRIFNTVRGRILVSFLAMGLLVLGGVSYVAQRQSAAALLASAE
                     DEGIALVRTLQEGFEQYLTARTAFMEAAAGHAEMKSGDWERQRAFLSSLDAKGMQIQA
                     YFLIDLQGDGHYLDGKVNKLGDREYFQRARDTRKTVVGQPVLSRATGETVVIVASPVF
                     SEKGELLSVLCGRITANTLITLASTQKWGESGHALVTDANGLFIVHPDKEFIGAVNAT
                     QAGEKIPEALAAAAKRALGGEQGIARYVEGGAEQVAAYSRVPSTGWSVAMAASVDEFL
                     APVRAMRNVILTSLLVAALLVIAVSFWIAASVSRPLARVVAKMDELATGDFSSSFEPR
                     SSLVEMRRLSSSFNGMIASVADFAGGVFGVSRKLLARAEDMSAASEQSSASVHEVIGL
                     VGKVAQNSHDAASAIEEANAGVGEVAHGAQAGAKAAVETGERAQEISTAAETGGSALE
                     EMVHLIGEVSRSGASVNAAVNNLASSVSGITGFVNTITQIADQTNLLALNAAIEAARA
                     GEAGRGFAVVAEEVRKLAEESNRAAGEVGRVIGEISQKTEHALADQKGSVEQIRQLVV
                     RAKETKAVIDDVVLKVGAITENVQSIAATMQEQSASAEEMTAGMDHVARSGAAIAEQV
                     ENINRSMDEQGRMTESIASTAVDLVDLSEELQRSVARFKTTAEGTGLALKK"
BASE COUNT         1920 a         3384 c         3165 g         2044 t
ORIGIN      
        1 catctactac gccacgggga gcagaagaaa ggcgttcaac tactccttcc tctccggagt
       61 ctcggaacca atcggcgcgc tgctggcgtg gctcgttctg gcgccctttc tgagcggagc
      121 gctcttcggc gtcatcttcg cctccgtcgc ggggatcatg gtcttcattt cggtggacca
      181 gctgcttccg gccgcccgca agtacggcga acatcacgtt tccatctacg ggctcgttat
      241 cggcatgatg atcatggccg tgagcctgct gctttttata taaaggggga gtttgcatgg
      301 ggataacgct caacgtcgaa cagtatctga aagatcttga atacctcgtg aacatcgaca
      361 gccagtcgaa ggatcccgag ggagtcgcga aggtcgccgc gttcttcgag aaggcgttcg
      421 gcgacatcgg ctggaccgtc gaacgggtgg ttttcgaccc gaccgcaggg ccgagcctga
      481 agatcaccaa cggagagcct ccgtatgacg cgctgctgct ggggcacctg gacacggtct
      541 tcccgaaggg aaccgtggcc gagcgtccgt tctcgcggga tgaaaagcgc gcctacggac
      601 cgggcgtcaa cgacatgaaa tcggggctgc tcttcggcct ctacgccgct cgggcgctca
      661 cagcgaaggg aagcaagggg tcgttctgca tcgcctacaa cagcgaggag gagatcggct
      721 cccgacgcgc gcgcccttgg atcgagcagc tggcgcagga gagccgcacc tccgtcattc
      781 tcgaaccggc ccgtcccaac ggcaacctga tgaacgcccg caaggggctg gggcgcatcg
      841 aggtgacgtt caacggaaag gccgcacacg ccggagtgga gccggaaaag ggggtcagcg
      901 cggtcaacga gatggcgtac tggatcatcg cgctccacgg gctcaccgac ttcgagaagg
      961 ggacgacgct caacgcgggc atcgtcgccg ggggcacggg cgcgaacgtc gtcccggaga
     1021 aggcgaccat gacggtcgac gtccgcgtga agataccgga ggaggcggga cgcatcgccg
     1081 agaagatact ccagctgaag gcgcaccccg ccacgccggg aatctcggtg gacgcggatt
     1141 tcatcctgac ccggccgccg atgaaccccg gtgagaagac gccgaagctg tgcgcgctcg
     1201 tcgaggaggc ggggaaggag gcgggcgtcg agttcggctg gcagtcgacc ggcggcggct
     1261 ccgacggcaa cttcacggcg gcgctcggcg tgccgaccgt ggacggcctc ggcgcgatgg
     1321 gcgggggctc gcactccatc ggggagtata tcgaaatcga cgcgcttcct tcgcgcttcg
     1381 ccatgctgct cggacttctg gagcgcatcc ctgacgccgg attctaaacc atgaacatga
     1441 agggaatcat cctcgccgtc tgcaccgctc cggcgaaggg attgcagaaa cacgatcagg
     1501 gggaagggcg tcttgtcccc ggcaagggga tcgacggcga cggacacttc ggcttcgccc
     1561 accggcaggt gagcctgctg gagtcggaag agatcgagaa gatgaagaag gtcatcccca
     1621 cgctcttcca cggagcgttc gccgaaaacc tggtcaccga ggggatcgat ctcaaaagac
     1681 tcatcctcgg cgacacgctc cgcatcgggg acgcgctgtt gcgggtgacg cagatcggca
     1741 aggagtgcca ctcgcactgc gccatctacg aggctgcggg cgactgcatc atgccgcgcc
     1801 tcgggatctt ctgcgaggtg ctctcggagg ggacggtaac ggtcggcgac acgatcgagt
     1861 tcgtccatga ggacgctccg caggtgctcg tcccgcggac gatcacgcgg ctcgacggaa
     1921 cgaagaggga gaccgtcacc gacagactgc tgaaggaagg gcgggtcact ctcgttctcg
     1981 acggcgaaga agaggttacg gccgtctgta cgcctggcga ggagcgctac tgggccgtcg
     2041 gccacttgaa gtgccgccgc cttttgaaaa gtcgcgaaga tattgcgagt ctcgaaataa
     2101 cccccggcgc ggtctcggtg aagcgcaagc agatgacgga ggggctgccg ctcttgcagc
     2161 gaatcctctc ctcgtcggcg tcggcgctgt tggccgaggg ggcggaggaa cagctcgggg
     2221 gagtcatccc ctcggagtgg cgcatccccg cctcggtgct ccgcgagggc gcggagtggc
     2281 tgagcgaagc ccccatcttc cgcgcaaccg gcggcacgca cgttgccgcg ctgatggcga
     2341 gggacgggga gaagctcttc cgcaccgagg acatcggacg gcacaacgcc gtggacaagg
     2401 cggtcggatg ggcggtgctc cacgacgtcc cgctcgggga gagcgcgttg ctcgtctccg
     2461 gcaggctccc cgtcgacatg gtaatgaagg cgctcggcgc gggcatcccc gtgctgggga
     2521 gcgtctcggc ggccatcgcc gagggcgcgg aggcggcgga gaagggcgga ctcaccctcg
     2581 cgggcttcat acggggggag cggatgaaca tctacacgca cccggaacga atttcggact
     2641 gaaaaacggc ccttcacggg gccgtttttt gttgacagat tgccgaaaag gcgatagtgt
     2701 agttgtggtt catctaaaga accgacgttt caacaagcga aattatatgt cgttaaggag
     2761 gtagagagaa tgtcccgatt cgtcacgttc ggagagatta tgctgcgcct cacgccgccc
     2821 gaacacgagg tgctcttcca gacggaccgt ctggtcgcga ccttcggcgg cgcggaggcg
     2881 aacgtcgccg tctcgctcgc caactacgga gagaacgtct cgtacgtcac cgccgccccc
     2941 gccaacccgg tgggcgacgc gatgatcaag gaagtccgct cgttcggcat ctgcaccgac
     3001 cacatcctcc gcaaggggag cggctgggca tctacttcac cgagacgggc gcggccatgc
     3061 gcccctcgaa ggtggtctac gaccgcgcgg gctcgtccat cgccgccgtg cagcccggcg
     3121 acttcgactg ggacaagatt ctcgacggcg caacctggtt ccacaccacc ggcatcaccc
     3181 cctcgctcgc cgaagggacg gccgccgtgg cgctggaggc cgtgaaggcc tgccgcgcga
     3241 agggaatcac cgtgtcgtgc gacctgaact accggaagaa actctggaag tgggggaaga
     3301 gcgccaaaga ggtgatgacc gagttcgcca agtacgtcga cgtgaccatc gcgaacgagg
     3361 aggactgcca gaagtgtctc gggatcgagc tcgacgtgga cgtgacgtcc ggcgcgctgg
     3421 acaccgcgaa gtacaaggtg ctcgcggaga aggtgatggc cgcctacccg aatatgaagt
     3481 acctcgccgt cagcctccgc gagagcgtga gcgccgactg gaacggctgg tccgcagtgc
     3541 ttaccgacag caaggggaac ttctacacca gcaagaagta cgagatccgc gacatcgtcg
     3601 accgcatcgg cggcggcgac tccttcggct ccggcctcat ctggggcctg aacaacctga
     3661 agacgccgca ggacgccctc gaattctccg tggcggcctc cgcgttgaaa cacaccgtct
     3721 acggcgactt caaccgcgtc tccaaggagg acgtcctcac cctgatgggc ggcgacgcct
     3781 cgggacgcgt acagcggtag aagctcgagc gctcggacga cgaaaaagcc gctgtggggg
     3841 aaatgcagcc tcagggacag ccgcctacaa cgccgatcgg tgcgcgccga tttccgcgtg
     3901 cggttttctc ttcaaagcga caggggcctc cgactactcc ggaggcccct gttctttcgg
     3961 gacgggaggc tacttctgca gcaggtggaa gatgaagccg ccgatgtgga ggtcgaggta
     4021 gagcgcgacg gtgcggccat ccttcttctt ctccgtctcc gggagggagg cgatcccctt
     4081 ccgcttcagg aaggcggcgg cgcgcatgac gtcgttgcag ccgacggcga cgtgcccctt
     4141 ctcgccgagg tacatgctct tcatgaactc gaagcccttc ccgacgaagg cggagctgtt
     4201 cccgtccttc accgccatgc cgaagagggc ggcgagcctc tcggcgccgg caagcgcctc
     4261 tttttcatcg ggacagttga ttcccaggtg gatcagctcg aagccgtgca tcgcgaagac
     4321 ggcttctctg gtcatgcggg cgatctcgtc gaacttcccc tccgcgacga cctcgggctt
     4381 caccatccag ctcccgccca cggcgaggat cttgttgaag gcgaggtagg agggcatgtt
     4441 cttcgcgtcg acgccgccgg tggggatgaa gcggacgctt ccgtacggag cgctcatcgc
     4501 cttgagcatg ggcagaccgc ccgactgctc ggccgggaag aacttcacca cgtcgagccc
     4561 gcgctccagc gccatctcga tctgcgacgg gctgttgatc ccgggggtta tggggatgtt
     4621 gtgctcgacg cagtagtcca cgaccgaggg gttgaacccc ggcgtaacga tgaacttcgc
     4681 ccccgcgtcc acggccttct tcacctggtc gcgggtgagc acggtcccgg cgcccaccag
     4741 cagctccgga agctcgcgcg acatgatgcg gatggactcc tccgccgcgt cggtacggaa
     4801 ggtcacctcc gcgatgggga gttccccttc aagaagcgcc ttgccgagag ggagagcgtc
     4861 ggaggccttg tcgagtttga ccacgggaac gatgccgagt tcccctatgc gctgcagtat
     4921 gtcgttcata ttatttttcg tctcctttcc tatgtggctg acgcgtcaag catagacaat
     4981 gaatcgccct ttcgtcaagg acttttttcc gcagaataaa acattgtttc gaaattcgcg
     5041 gcgagatttt cggccggtag ggagacgttc ccaaaaaaag cgaataaaaa agaccccgta
     5101 tcgctacggg gtccgccatg cattgggttc gcgctactca accgactggt agagcgcttc
     5161 ctgctcctcg accgagaaga tgcggtcgag atcaaggaag atgatcagcc tgccatcgac
     5221 cttggccaca ccccggatga agtccgagct gacgccgctg gccacgatgg gaggcgtcgg
     5281 ctcgatcgac tcgtccggga tgcgaagtat ctcggagact ccgtccacga ggatgccgat
     5341 gttctgtccc gacatgttga cgacgatgat ccggcgttcc gtatcgctgg agccgcttct
     5401 gtccgcgacc agaaacctct tgcacagatc gacgatcggg acgatctgcc cccgaagatt
     5461 gactatcccc tcgacgaagt ccatggactg ggggatggtg gtgatgttct gcagtctgac
     5521 gatctcccgg acgcgggaga tgtcgatgcc gaattcctcc ttgccgagag cgaacacgac
     5581 aagctgttgt tccgccattt tttttcctcc ttccgcaaga tcctgctgaa taaactcttc
     5641 aggacaattc taacagatat tgtgatgaag agcacccgaa tcccgcaaaa tcacacaacg
     5701 gttttatgcc gcgcgcccgc gtcacttttt cagcgcgagc ccgctccggc gcgcatcgtc
     5761 cacgataaat cgcgagacaa gcgtttgcag cttctcggac gttaccgcca tcgcctgcgc
     5821 ctcggtcgcg atcgattcgg cggccttcgt cgtttcgtgc gacgctcccc ggatagagcc
     5881 gaccgagctc acaacctgtc cggtggattc ggtgacgctc tgaatcgccg aggtcatctc
     5941 ctcgctcgac gcggcctgct cctccgagac ggcggcgatg ttctgcaccg cttccgcgat
     6001 cttgacgatt gcgtccacgg cgcccttgag cttctgccgc atctcttccg cgcccttcat
     6061 cgtatcgtcc agtatagttc ccgtcttttc cgtggctgcg atggagtcgt ccgaatgctt
     6121 ctgaagctcg ttgatgagct tgcttacctc gtgcgccgcc ctggccgact cctcggcgag
     6181 cttgcggacc tcttcggcca ccaccgcgaa gcctcttccg gcctcgccgg ctctggccgc
     6241 ttctatcgcc gcgttcagcg ccagcaggtt cgtctggtcc gctatcgacg tgatggtcgt
     6301 cacgaatccg gagatagcct ccaccgactt gccgagctcc ttgatccggt cgatgctctt
     6361 cgcggactcg ctccccgcgt ttcgtatgtt gttgattgcg cgtcctacct cctgcagcga
     6421 agcgttcgcg gcgtcggagg cgttcgtcgc gccgtccgct ccttcgttgg tcgcccttgc
     6481 cgcagtctgc gcgcccgccg cgatctcctc tatcgacgcg ttcgtctcct gcagcgcgga
     6541 ggagctgtgc tccatcatgt cctggacttt ttcaatggag ctggagacct cttccatgga
     6601 cgcaagcgtc tcctcggaga gggaagcgag cgtctcggcg cgcgcggccg tctcctgcga
     6661 ctcgtttcgg atcgacccca tgacttcgcg cagcgagaag accatgccgt tcagaatgcc
     6721 ggaaatatgc gcgatctcgt ctttgcccga ggcctcgtac gagaccgtca ggtcgccttc
     6781 tcccagcttc tccgtcacac tctccatcga gcgtatcgac ttcgtcaggc cgcgcgcgat
     6841 gaagaagatc gtccctcccg tcaccaggac ggcgacgacc gcgagaccga gcagaataaa
     6901 ggtgagcgcg ttcaccatcg cgttgatctc cgcggcgggg aagaggatgc cgaggtagaa
     6961 agcgcgctgc gtaggggcga agaacatctg ccgctcctcc ccctggtaga cgtagctctc
     7021 atatcccgtc tcgccggaga ccatcttccg cgcgatattc cgtatcggct cggcgaactt
     7081 ctcgtcggtc atcatgttcg ccttcaaaac gtcgtctttc tcattgtagg caacgaagat
     7141 gccgcttttc aggagcatca ggccgcttcc cttgccgaaa atctggagcc cgacgacgta
     7201 gtcgctcagg ccgctgatgt ccatgtcgcc cccgacgact cccaggagtt ttccggcgtc
     7261 atcgtagata gccgtggcgg tggagatgac gacctgcttc gtgatcccgt cgacgtaagg
     7321 ctcggtgaag atgatctttc cctttcccgc cgccaccgct tccttatacc acgagcgggt
     7381 tctgcagtca tagtcgtcgg gctccttcca gccggtgccg tcggagaact ttcccgtcgc
     7441 ctcgtgcccc atgtagagat ccatgatccc ctcgtttttc gtccgggcag tcaaggcggc
     7501 gacgatcttt tccacgccgt cctcatccgt cacgttcagc cggagcatcg cgtcgcggac
     7561 cgtctccgcg gctgtaagca cgatcgcctc gaccttgttc agacgcgcgt cgacattctc
     7621 ggcggcgctc ttcacgatct ccacgccggc cgtgtccacc aggccctcga taatactccc
     7681 ggcccggtag tacgtcactc ccgccatcgt ccctatcatg gcgagcacca caagcgccat
     7741 caccagcagt ttgttcctga tcgtcaattt ttttccccct cttccgtgat attatcggaa
     7801 cttcctctcc gcgcacatga agcctaaagc agcgagcgga gcatttcccc ccggcacgtt
     7861 cccctgaaac gcttctcagc aatgaagaaa agaatttcct cctttcagaa aagcttctac
     7921 tttgttcaat tcaacacaag gattgctttc attcgtcttt tgaaaggcag tcaaacaggc
     7981 ttcatttccg tatcggccct ttccttccgc caaagaagtt tctgaagttt taatctatag
     8041 aacgggagct tgcgcagccg tttccggcgg tcattcctga cccgccccga gtcctcgaag
     8101 aggcttcgct tatcgccgaa atcgcgccgc ggagaaccgt cataaaaaag gggggagaag
     8161 gcatacgttt gcctcctccc ccctcgtttc tacgttgtga aagcccaggt cactttttca
     8221 gcgcgagtcc ggttccttcc gccgttgtct tgaagcgggc cacggagcgc tggagctcct
     8281 ccgagagatc gacaaggtct accgccgtgg acgcgatgct ctccgtcatg cgcccctgct
     8341 cgtccatcga acggttgatg ttctccacct gctcggcgat cgccgcgccg gagcgggcca
     8401 cgtggtccat ccccgcggtc atctcttccg cgctcgccga ctgctcctgc atcgtcgcgg
     8461 cgatggactg cacgttctcg gtaatggccc ctaccttcag caccacgtcg tcgatgacgg
     8521 ccttcgtctc tttcgcccgg acgacgagct gccgtatctg ctccacgctt cctttctgat
     8581 cggcgagcgc gtgttcggtc ttctgggaga tctcgccgat tacccggccg acctcgcccg
     8641 cggcccggtt ggactcctcg gccagctttc tgacttcctc ggcgacgacc gcgaagcccc
     8701 gtccggcctc tccggcgcgc gcggcctcga tcgccgcgtt cagcgccagc aggttcgtct
     8761 ggtccgctat ctgcgtaatc gtgttgacaa agcccgtaat gcccgacacc gacgaggcga
     8821 gattgttcac cgcggcgttg acgctcgctc ccgaacggga gacctcccct atcaggtgca
     8881 ccatctcctc cagtgcgctg ccgcctgttt ccgcggcggt ggaaatctcc tgcgcacgtt
     8941 ctcccgtctc gacggcggcc ttggcgcccg cctgcgctcc gtgggccact tcgccgacgc
     9001 ccgcgttcgc ttcctcgatt gcgcttgccg catcatgcga gttctgcgcc accttgccga
     9061 caagaccgat aacctcgtgc accgaggcgc tgctctgctc cgaggccgcg ctcatatcct
     9121 ccgcgcgcgc aagaagcttc cgcgagacgc cgaagacgcc tcccgcgaag tccgcgaccg
     9181 aagcgatcat cccgttgaac gaggaggaaa ggcgccgcat ctccacgagg ctggaacggg
     9241 gctcgaagga agagctgaag tctcccgtcg ccagttcgtc catcttcgcc accacgcgcg
     9301 caaggggcct ggagacgctt gcggcgatcc agaacgagac ggcgatgacc agcagcgccg
     9361 ccacgagcaa cgacgtcaga atgacgttcc gcatggccct gacgggcgcg aggaactcgt
     9421 cgacggatgc ggccatggcc acgctccatc cggtcgaggg aacgcgcgaa taggctgcaa
     9481 cctgctccgc tcctccctcg acgtagcgcg cgatcccctg ttctcctccg agagcgcgct
     9541 tcgcggcggc ggccaacgcc tcgggtatct tctccccggc ctgcgtcgcg ttgacggcgc
     9601 cgatgaactc cttgtcgggg tggacgatga agagtccgtt cgcgtcggtg acgagggcat
     9661 gcccgctctc gccccacttc tgggttgaag cgagggtgat cagcgtgttc gccgtgatcc
     9721 ggccgcagag cacggagagc agctctccct tctcgctgaa gacgggggag gcgacgatga
     9781 ccaccgtttc tccggtcgcg cgggagagga cgggctggcc gacgacggtc ttccgggtat
     9841 cgcgggcgcg ctggaagtac tcgcggtcgc ccagcttgtt caccttgccg tcgagatagt
     9901 gcccgtctcc ctgaagatcg atgaggaaat acgcctggat ctgcattcct ttcgcatcca
     9961 gagaggagag gaaggcgcgc tgacgctccc agtcgccgct tttcatctcg gcgtggccgg
    10021 ccgcggcctc catgaatgcg gtccgcgcgg tgaggtactg ctcgaacccc tcctgcagag
    10081 tacgcacgag ggcgattccc tcatcctccg cggatgcaag aagcgccgcc gccgactgac
    10141 gttgcgcgac gtagctcact ccgcccaaaa ccagcagtcc catcgcgaga aaggaaacga
    10201 gaatccttcc gcgcaccgtg ttgaaaatcc tgttcatacc ctctcctcct tatcggccct
    10261 cgggccgtgt ttgaaacgga acacaaaaaa cattaaaaca cctcgctgat ttttcggaac
    10321 ttttcgcaag gtgtacaccc tgtaatagat attctccata taatattcac attccatttc
    10381 cttgtaaaac tatcacatgt atggactttt gtaaaggact tgcgaacctg ttttatacac
    10441 agatgcctat tgaagctctg cgttgaacga aagacttttt accaaaaacg acggaagaga
    10501 gcgtcgcgcc ctc
//
LOCUS       DAOPKU010000026        10478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_6666_length_10478_cov_4.892545, whole genome shotgun sequence.
ACCESSION   DAOPKU010000026 DAOPKU010000000
VERSION     DAOPKU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10478
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6666_length_10478_cov_4.892545"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="PK849_01555"
     CDS             complement(<1..2056)
                     /locus_tag="PK849_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE64838.1"
                     /translation="MRRERVIKQLGRSLPAFSAAALLFLCIAVCAACLFAFPSDASLM
                     EDFRSSGEAVELSGEERDYLAALGPVTVCPDPDWMPFEHVDEQGNFTGIAADLLELIA
                     GRLGIEFSYVFPKDWSEAVALSKSGDVLILPFLNKTPAREEWLLFTEPLLVDPNVFIA
                     REEHPYIYDASQLTDRTIVLPEGTSMEERLRRDFPNLDVITVEREEDVFRAVVERRAD
                     MTLRSLTIAAWTIRKEGYFNLKVVGQAPEEYVNRLRMGVLKSEPRLRDILNKGIAAIT
                     PSELEAIVNRHVNITVVRQMDYWFVIRIAAALGLFVAFSFYWNARLRKSRAALEESER
                     SKSVLIANLPGIAYRCRYDEEWTMEFVSEGCLRLTGYASDDLLFNKKISFNKLMLPEY
                     REYARAVWERAMAERVPAALEYRILTAEGEEKWVFEQGVPLFDETGAVEFLEGLIIDI
                     SSRKALEDRLQSTLGLQGLLAEISASFINATLANIDQKIDAMLERCGEFLKVDRTFLF
                     QLSEDCTLMSNTHEWCAPGIPAVKDDVQNYPVADVPLIEKTICAQEMLFIPDVSELSE
                     EWSAEKRELERRQVRSVLCLPLIKGGRFLGYFGFDAVRATKQLDDETVEVLKILANTL
                     ADALESNRIEKEMLEAKTRAEDATRAKSEFLANMSHDIRTPMNGVLGMTALLLDTQLS
                     EEQ"
     gene            complement(2056..4260)
                     /locus_tag="PK849_01560"
     CDS             complement(2056..4260)
                     /locus_tag="PK849_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE64839.1"
                     /translation="MTKRKQADENCPGSAGLAAGSCGEETLQEENRRLKLEAAVAREA
                     ASKAMAASIAKSEFLANMSHEIRNPMNGVIGMTGLLLDTRLNGEQRYYAGMVRDSAVS
                     LLSILNDILDFSKIEAGKLELETLDFDLPSLLQDFSATMSLRAREKNVALLCSIAPDV
                     PSLLRGDPGRLRQILNNLVGNALKFTQAGEVELGVFLEEESENFALLRFSVRDTGIGI
                     PEERLPGIFDKFMQVESSTARTFGGTGLGLAISKQLAEMMGGRIWATSVEGKGSEFCF
                     TAALGKQYEQSVPDISSVDVRGVRVLVVDEKPGSFKLLTTRLLSWGMRPSEVSDGASA
                     ISLLREAVRAKDPFLLAVMDTQTGDMDGETLGRLIRGDELLSATRTVMLASIGVRGDA
                     KRFADAGFSGYLSGPALQNDLHDIFSLVLSEQRKNTRFIATRHTVREALPPFDGKGAH
                     VLLAEDNVTNQRVAIGMLKKFGVTVDVVADGLDALRLLRKGGYDLVLMDCQMPKMDGF
                     ETARRIRSMEGPRRDVPIVAMTAYAMVGDREKCLQAGMNDYIAKPILPALLVDTLKKW
                     LPEREKERTSPEKEGGPPVAKPKKTNAPKQKKTALRVPAAGAPAAPEQQAWNRGLLLE
                     RLMGDEELVMEVLQDFLSELPRELRKLGDVLSSDNLPLMERHAHSIKGMSACTGAEAL
                     ASVAFKIEERAREEDAEGARSFLPALKKEWKRLKTELEEERAPRNHSRRTRT"
     gene            complement(4495..4704)
                     /locus_tag="PK849_01565"
     CDS             complement(4495..4704)
                     /locus_tag="PK849_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPE64840.1"
                     /translation="MESVYKVIELVGTSSESWEKAAAAAVELASKSLRDLRIAEVTAM
                     DMHLEDGKIMAYRTKLKVSFKFEDK"
     gene            4936..5457
                     /locus_tag="PK849_01570"
     CDS             4936..5457
                     /locus_tag="PK849_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPE64841.1"
                     /translation="MESVGCVHVYTGTGKGKTTAALGLMLRAAGAGLSVLLVQFLKRA
                     EYSEHEALKLLGDRVTVRTFGGERRIGAPATDEDRKLAREGLAFLGTALASGAYDLVV
                     ADEIVVAAHIGLLLEDEILALLDVRPPSVELVLTGRYAWEELMERADLVTEMQEIRHY
                     FAKGVPARRGIEK"
     gene            5454..6836
                     /locus_tag="PK849_01575"
     CDS             5454..6836
                     /locus_tag="PK849_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HPE64842.1"
                     /translation="MTHPRIVIAGTNSGSGKTTFTAGLISALRSRGMTIQPFKTGPDY
                     IDPGFHTLAAGRPCRNLDTMLLSRDGTLELFDRNSSGADLSVVEGVMGLFDGAGARDE
                     RGSTAHLAKMLKAPVLLVVNGRSMARSAAALVLGFSKFDRRVAVAGVLLNALGSANHY
                     RILKEAIEGDTGIPVFGYLPRTADIAVPERHLGLVPSVEHEQGAEMIERIRLLIEEHV
                     DVKALLELARRAPLLPSYSPAIFAPPATPRGARIAVARDEAFHFYYQDNLDILEHLGA
                     TLLPFSPLRDPKLPEGTQGVYIGGGFPEEFAEKLSANESLRAELRAAADRGMPLFAEC
                     GGLMYLVERLEDKSGTAHRMAGVFPGVTWMGKRLQALGYCGGTLERGALIGRKGASLR
                     GHLFHWSIYDSEGNDELFSLRLEKNGNVYRDGLSRKNAFASYLHLHFGTNLAPARRFL
                     ERAAKFTGSV"
     gene            complement(6955..>10478)
                     /locus_tag="PK849_01580"
     CDS             complement(6955..>10478)
                     /locus_tag="PK849_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLAT/LH2 domain-containing protein"
                     /protein_id="HPE64843.1"
                     /translation="FGEFDLVEEGISVCKLHSPSCLQILDQIDGKYLLSLRGGRLAEQ
                     GYSGDSYTYLLTTPSGAVTVLVKNSRHFSGWFAPDGGIVLGDWGRPAGIRRYSPLGVP
                     EGEFALAECRTRYGFFVSQSEMLFPSDFFPYTFIKVDTLTGVCTGMYPFSPGMTMHFA
                     DSFSRDGKVYSFSSSRPSISSPLRGANLICFDASGQGWLDIAMAPLDPHAGAPMSTFE
                     QTEVAVRFLHEYGRSFGNQLDVTFGGGTVRSADPEGGRYVWETPPTGGAEFVVVPVEA
                     RLGPVSRVFEVTLRRLRNPLQLAVTKRLQTDRNGHIKLFADWELTNPAPYDLNDLAWS
                     FSGTNLKLSGVSGLPRSMRAGETRSGAAEVDMLYEGMESEPSVWGYSVEGRGSLSVTA
                     GRGGPVSGEIAHSFFVPKMYGFRLELFDPELNRRAPLEYHLRGLRVFDEEGNDITSSL
                     TYSKRSTFDLFTGVAAGLPGKPVRLRVRVHEAEAVVDLQFADNTHPKETHPWNIPSPV
                     ARITLGPNKSPVADFRIRPQDPKWSPETILDASPSYDPDGIITRYSWTLEAGSPPDAR
                     NDSVIAHRFPAGGVQTVTLSLTDNKGATSTVTKRVHTSKPFTVRGDEFAAQSVFAVPP
                     TASYEVTVRTGDRVKSGSDAGVFLALYGPADEQGTPTGSGEIHLLPSSSLQCTSTDPF
                     ERGGTDVFTIPEIYGFGSLDEAERMTLRHDNGGDGAGWFVDGVQVKNLKNGKSWTFPA
                     NRWLATDEGDGKTYAEFTPITGAYERGILFGGDARSIGMIEASDRIFILPAGASSFYF
                     TQLEGSRELRVYRNDVLVGTHSQAGRGLRDPLFLPESGWGVSYDASLITAPARFRVQT
                     LDSGSVREDGSVWVLPQSWAGHEKTVRQAVMADALIRELRSGLSGEDPYGVFACGKSA
                     VKVFAAARPNLGVAGVKILRYGMSSLSIFGDFDDVQFDLFTELASNAYFSSKLPALVA
                     QLLTGTFGSVTTEMLSLLKSIFNAAEWGAEFFSTSIFDAPSDVYAVDLLNDIAVSDSF
                     FLQSAELLQASGEKLRAAVSAFEQNDPEACAAAFGSMSTLVVGDAPESETLADHRVDY
                     GTGVDNCINHPDTQYPLVVLLAVKLQNIESWRRDGHSFLNHYYNPDAPGLDPIMATRT
                     AMVPFEKAIRDMLRIAGGLAQICLVGR"
BASE COUNT         1999 a         3382 c         3091 g         2006 t
ORIGIN      
        1 gctgctcttc gctcaactgt gtgtccagca gaagcgccgt catgccgagc accccgttca
       61 tcggcgtgcg gatgtcgtgg ctcatgttgg cgaggaattc gctcttcgcg cgggtcgcgt
      121 cctcggcgcg ggttttggcc tccagcatct ctttttcgat cctgttgctc tccagagcgt
      181 cggccaatgt attcgcaagg atcttgagca cttcgaccgt ctcgtcgtcg agctgcttcg
      241 ttgcgcggac cgcgtcgaaa ccgaaatatc cgaggaaacg gccgcctttg atcagcggca
      301 gacagagcac agaacgcacc tgccggcgtt cgagctcgcg cttctccgcg ctccactcct
      361 ccgacagctc cgagacgtcc gggatgaaga gcatctcctg tgcgcagatt gttttctcga
      421 tcagcgggac gtcggcgacg gggtagttct gcacgtcgtc cttcaccgca ggtattccgg
      481 gagcgcacca ctcgtgcgtg ttggacatga gcgtacagtc ctccgaaagc tggaagagga
      541 aggtcctgtc caccttgaga aactcgccgc aacgttcgag cattgcgtcg atcttctggt
      601 cgatgttggc gagtgtggca ttgatgaaag aggcggaaat ctccgcgagc agcccttgga
      661 gtccaagagt gctttgaagt cgatcctcaa gagctttccg cgaagagatg tcgatgatga
      721 gtccttcaag aaactcgacc gctcccgtct cgtcaaagag gggaacgccc tgttcgaaga
      781 cccacttctc ctccccctcc gccgtcagga ttctgtactc cagcgccgcc ggaacgcgct
      841 ccgccatcgc tcgttcccag acggcgcggg cgtactccct gtactccggc agcatcagct
      901 tgttgaacga gatttttttg ttgaacagca gatcgtcgct ggcgtacccg gtcaaacgga
      961 gacacccttc ggagacgaac tccattgtcc actcttcgtc gtagcggcaa cgataggcaa
     1021 tccccggcag gttggctatc agcactgatt tgcttcgttc gctctcttca agcgcggcgc
     1081 ggcttttccg gaggcgcgcg ttccagtaga aggagaaagc gacgaaaagc cccagggccg
     1141 cggcaattct gatgacgaac cagtaatcca tctgccggac gaccgtgatg ttcacatgtc
     1201 ggttgacgat cgcctccagc tcgctcggcg tgatcgcggc aattcctttg ttcaggatgt
     1261 cgcgcaaccg cggctcgctc ttcaggacgc ccattcggag acggttgaca tactcctcgg
     1321 gagcctgccc gacaaccttc agattgaagt agccttcctt gcggatggtc caggcggcga
     1381 tagtcagcga acggagcgtc atgtccgccc gtcgctcgac gaccgcgcga aaaacatcct
     1441 cttcgcgttc gaccgtgatc acgtccaggt tgggaaagtc gcggcggaga cgctcctcca
     1501 tcgaggttcc ctccggcagg acgatcgtcc tgtccgtgag ttgcgaagcg tcgtagatat
     1561 aggggtgttc ttcgcgggcg ataaagacgt tcgggtccac cagcagcggt tcggtgaaga
     1621 gaagccattc ctcccttgcg ggagtcttgt tcaggaaggg aagaatcaga acgtcgcccg
     1681 acttggagag cgccacggcc tcgctccagt ccttcgggaa aacgtaggag aattcaatgc
     1741 cgaggcgtcc ggcgataagc tccagcagat ccgccgctat tcccgtgaag tttccctgtt
     1801 cgtcgacgtg ctcgaacggc atccagtccg gatcgggaca gactgtgacg ggaccgagcg
     1861 ccgccagata gtcccgctct tccccggaga gttcgacagc ctcccctgac gagcggaagt
     1921 cctccatgag cgacgcatcc gagggaaaag cgaagagaca ggctgcgcac acggcgatgc
     1981 acaggaaaag aagcgcagcg gcagagaacg ccggaagaga acgcccaagt tgcttgatga
     2041 cgcgctcccg tcgcattacg ttctcgtcct cctgctgtga ttgcgcggcg ctcgttcttc
     2101 ttccagctcg gttttcagcc gcttccactc cttttttaac gcgggaagga agcttcgcgc
     2161 accctccgcg tcttcttctc tcgcccgctc ctctatcttg aaagcaacgg aggcgagagc
     2221 ctcggccccg gtgcatgcgc tcatcccctt gatactgtgc gcatgccgct ccatcaaggg
     2281 gaggttgtca gaggagagca catccccaag cttccggagt tcgcggggaa gctccgacag
     2341 gaaatcctga agcacttcca ttacaagctc ctcgtctccc atcaggcgct ccaggagcag
     2401 ccccctgttc caggcttgct gctccggtgc agcgggcgcg ccggccgccg gaacgcgaag
     2461 agctgtcttc ttttgcttcg gcgcgtttgt cttcttcggc ttcgcgaccg gaggcccgcc
     2521 ctctttctcc ggagacgttc gttctttttc acgttcagga agccattttt taagcgtatc
     2581 gaccagaagc gccggaagta tcggcttcgc gatatagtcg ttcatcccgg cctgaagaca
     2641 cttctcccgg tcgccgacca ttgcatacgc cgtcatggcg acaatcggca catcgcggcg
     2701 cggtccctcc atgctccgga ttctgcgcgc cgtctcgaag ccgtccatct tcggcatctg
     2761 gcagtccatc agcacgaggt cgtagccgcc ttttcgcagc aaacgcagcg cgtcgaggcc
     2821 gtcggcaacg acatcgaccg tcactccgaa ttttttgagc atgcctatcg ccacacgctg
     2881 gttggtaacg ttgtcctccg ccagaagaac atgcgcgccc tttccgtcga aaggcggaag
     2941 cgcctcgcgc acggtatggc gagtggcgat gaaacgcgta ttctttcgct gttccgacag
     3001 gacaagcgaa aagatatcgt gaaggtcgtt ctggagcgcc gggccgctca ggtaaccgga
     3061 gaatcccgca tccgcaaagc gtttcgcgtc gccgcggacg cctatggaag ccagcatgac
     3121 ggtgcgcgtg gccgagagca gttcatcgcc cctgatgagg cgtcccaacg tctcgccgtc
     3181 catatcgccc gtctgcgtat ccatgaccgc aaggagaaag ggatcctttg cccgcaccgc
     3241 ttcgcgcaga agcgagatgg cggacgcgcc gtccgaaact tccgaggggc gcattcccca
     3301 agagagcagg cgggtcgtca gaagcttgaa actcccgggt ttttcatcca cgacgagcac
     3361 gcgcacgccg cggacatcca ccgaggagat atccggaacc gactgttcat actgttttcc
     3421 cagcgcggcg gtgaaacaaa attccgaacc cttcccctcg acgctcgtcg cccagatccg
     3481 tccgcccatc atctccgcaa gctgcttcga aatggcaagc ccgagacctg tccccccgaa
     3541 tgtccgtgcc gtggacgatt ccacctgcat gaacttgtcg aaaatgccgg ggagccgctc
     3601 ctccggaatg ccgattcccg tatcgcgcac cgagaagcga aggagcgcga agttctcgct
     3661 ttcctcttca aggaaaactc cgagctcaac ctcgcccgcc tgagtgaact tcaaggcgtt
     3721 ccccacaagg ttgttcaata tctggcgaag ccgcccggga tctccgcgaa gcaacgaggg
     3781 aacgtcggga gcaatgctgc acagaagcgc gacgttcttt tctctggcgc gaagcgacat
     3841 cgtcgcggaa aagtcttgaa gcagacttgg aagatcgaaa tccagagtct ccagctcgag
     3901 tttccccgct tcgatcttgg aaaagtccag aatatcgttc aggatgctca acaacgagac
     3961 cgcgctgtcg cggaccatcc cggcgtagta gcgctgttcg ccgttcaggc gcgtgtccag
     4021 caagagcccg gtcatgccga tcacgccgtt catggggttc cggatctcat ggctcatatt
     4081 ggcaagaaac tcgctcttcg cgatgctcgc cgccattgcc ttcgaagcag cctcccgcgc
     4141 caccgcagct tcgagcttca agcggcggtt ctcctcttgg agcgtctctt ctccgcaact
     4201 ccccgcggcg aggcccgcac tccccgggca gttctcgtca gcctgttttc tcttcgtcat
     4261 gtcgtcgctc ccgtgacgtg taagtttcgc tctccgagcg atccggcccg ctctccccgg
     4321 atttgatgaa ccagcgcttc cgctgttcat accacgcgct tcgtgcactc ggttcgatta
     4381 tatcatccgt tcttttcagg ctctatggga ggtcatcggg aatacgaagc cgcccgcccg
     4441 cttaaagaac tttatcgggc gggcggcttc ctggtacggc aacgcagggg catgctactt
     4501 atcttcgaac ttgaacgata ctttgagctt ggtccggtat gccataatct tgccgtcctc
     4561 gaggtgcata tccatggcgg taacttccgc gatacgcagg tccctcaacg acttcgacgc
     4621 cagctccact gccgccgcgg cggctttctc ccacgactcg ctgctcgtcc ccacaagctc
     4681 gatcaccttg tacacgctct ccatgtcatc tcctccttcc agatcggctc cgaagagccc
     4741 gccagtttcc gccggcaaag catcgccgcc ggaaaaaaga atagtttaat tataccatta
     4801 ccactcttca tttcactctg aataaatact tttcgggacg cttgcttctg cacgggttgc
     4861 gccgttatcc gcatcccggc gaacgtcctt ttccataccg tataatgaga gaaaaagcag
     4921 ggaggggctt cgtttatgga gagcgtcggt tgcgtgcatg tctacactgg aacgggaaag
     4981 ggaaagacca cggcggcgct cggcctgatg cttcgcgctg ccggggcggg gctttccgtg
     5041 ctcctcgtcc agttcctcaa aagggcggaa tacagcgaac acgaggcgct gaagctcctc
     5101 ggggaccgcg tgaccgtccg caccttcgga ggagagcggc ggatcggcgc tcccgcaaca
     5161 gatgaggacc ggaagctcgc gcgggagggg ctggcctttc tcgggacggc gctcgcgtcc
     5221 ggagcgtacg atctcgtcgt cgccgacgaa atcgtggtgg cggcgcacat cgggctgctg
     5281 ctcgaggatg agattctcgc cctgctcgac gtccgccccc cctcggtcga actggtgctc
     5341 acggggcgct acgcctggga ggagctcatg gagcgcgccg accttgtcac cgagatgcaa
     5401 gagatccgcc actacttcgc gaagggcgtt cccgcccgga gaggaatcga aaaatgacac
     5461 atccgcgcat tgtgattgca gggaccaaca gcggttccgg gaagacgacc tttacggccg
     5521 ggctgatctc cgcgctccgt tcgcgcggga tgaccatcca acccttcaag acggggccgg
     5581 actatatcga ccccggcttt cacaccctgg ccgcagggcg gccctgccga aacctcgaca
     5641 cgatgctgct ctcccgcgac ggcacgctgg agctgttcga ccgcaactcc tccggcgcgg
     5701 acctctcggt cgtcgaaggg gtgatgggac tcttcgacgg cgcgggcgcg cgcgacgagc
     5761 ggggaagcac cgcacacctg gcgaagatgc tcaaagcccc tgtgctcctt gtggtcaacg
     5821 gccgctcgat ggcgaggagc gccgcggcgc tcgtcctcgg tttctcgaag ttcgacaggc
     5881 gcgtcgcggt cgccggagtg cttctgaacg cgcttggaag cgccaaccac taccggattc
     5941 tcaaggaggc catcgagggc gatactggca ttccggtctt cggctacctg ccgcgcactg
     6001 ccgatatcgc cgtgccggag cgtcacctcg ggctcgttcc ctcagtggag cacgaacaag
     6061 gcgcggagat gatcgagcgc atccgtctgc tcatcgagga gcatgtcgac gtcaaggcgc
     6121 tcctcgaact cgcccggcgc gccccgctcc ttccgtcgta ctcccccgcg atcttcgccc
     6181 ccccggcgac gccgagaggc gcaaggatcg cggtcgcgcg cgacgaggcc tttcacttct
     6241 actatcagga caacctggac atccttgagc atctgggagc gacgctgctc ccattcagcc
     6301 cgctgcgcga cccgaagctg cccgaaggga cgcagggggt gtacatcggc ggcggctttc
     6361 cggaggagtt cgcggagaag ctttccgcca acgaatcgct ccgcgcggag ctgcgagcgg
     6421 cggcggaccg cgggatgccc ctcttcgcgg agtgcggcgg tctgatgtac cttgtcgaac
     6481 ggcttgaaga caaatcgggg acggcgcacc gaatggcagg ggtctttccg ggagtcacct
     6541 ggatgggcaa gcgcctgcag gcgctcgggt actgcggagg gacgctcgaa cgcggcgcgc
     6601 tgatcggaag gaagggcgcg tcgcttcgcg gacatctctt ccactggtcc atctacgaca
     6661 gcgaagggaa cgacgagctc ttctccctcc ggctggagaa aaacggaaac gtctaccgcg
     6721 acgggctgtc gaggaagaac gccttcgcaa gctaccttca tctgcacttc ggtacgaacc
     6781 tcgcccctgc gcggcgcttt ttggagcgcg cggcgaagtt caccgggagc gtctgaggcg
     6841 ctggacgccg cggcggcaca ggaaatctct ccgaaaacct gccgcctgtt tccggagaag
     6901 agacacacag tccgttcttc ccgtagcggc gaacgcactc cgttgtattg cgcttcaccg
     6961 cccgacgagg cagatctgcg ccagtccgcc cgcgatccgc agcatatctc tgatcgcctt
     7021 ctcgaacggg accatcgccg tcctcgtcgc cataatcggg tcaagcccgg gagcgtccgg
     7081 gttgtagtag tggttcagga aggaatgccc atcccttcgc catgactcga tattctgcag
     7141 cttgacagcg aggagcacaa caagcggata ctgcgtatcg gggtgattaa tgcagttgtc
     7201 gactcccgtt ccgtagtcga ccctgtgatc ggcaagcgtt tcgctctcgg gggcgtcgcc
     7261 gaccacaagt gtggacatgc tgccgaaggc cgccgcacac gcctccgggt cgttctgctc
     7321 aaaagccgat acagccgccc gcagcttctc tccggaagct tgcagaagct ccgcggactg
     7381 caggaagaaa ctgtctgaca cggcgatgtc gttcagcagg tcgaccgcat agacatccga
     7441 aggcgcgtcg aaaatcgacg tcgagaagaa ctccgccccc cactccgctg cgttgaaaat
     7501 gctcttcaga agagaaagca tctccgtcgt caccgagccg aacgtacctg tcaaaagctg
     7561 tgcaaccagc gcgggcaact tgctcgaaaa ataggcattg gaagcgagtt cggtgaacag
     7621 atcgaactgc acatcgtcaa aatcgccgaa gatcgaaagg gaggacatgc cgtaccgcag
     7681 gattttcact ccggcaaccc cgaggttcgg gcgcgccgcg gcgaacacct tcacagcgct
     7741 cttgccgcag gcaaagacac cgtacggatc ctctcccgac aggccgctgc gaagctcccg
     7801 aatcagagcg tccgccatca ccgcctgccg gaccgtcttc tcgtgtccgg cccagctctg
     7861 cggcagcacc cagacagagc catcctcccg gacggaacct gaatcgagcg tctggacgcg
     7921 gaagcgcgcc ggagccgtga tgagcgacgc gtcgtacgaa actccccagc cgctctccgg
     7981 gaggaagagc gggtcgcgaa gcccccgccc cgcctgggag tgcgtcccta ccagcacatc
     8041 gttccggtag acgcggagct cccgcgatcc ttcaagctgc gtgaagtaga aggacgaagc
     8101 gcctgcgggc aggatgaaga tcctgtcgct cgcctcgatc atgccgatgg agcgggcgtc
     8161 tcccccgaag agaattccgc gctcgtaggc gcccgtaatc ggggtgaact ctgcgtacgt
     8221 cttgccgtcc ccctcgtccg tcgcaagcca gcggttcgcc gggaaggtcc agctcttccc
     8281 gttcttcaga ttcttgacct gaacgccgtc cacgaaccac ccggctccat caccgccgtt
     8341 gtcatgacga agcgtcatcc gctccgcctc gtcgagcgaa ccgaatccat agatctccgg
     8401 aatggtgaag acatccgtcc ctcctcgttc gaacgggtcg gtggaggtac actgaaggct
     8461 cgaactcggg agcagatgaa tctcccccga tcccgtcggc gtgccctgtt cgtcggcagg
     8521 accgtagagg gcaaggaaga ccccggcgtc ggagccgctc ttcacacgat cgccggtcct
     8581 caccgtcacc tcgtacgacg ctgtcggggg cacggcaaag acgctctgag cggcgaactc
     8641 atccccccgc accgtaaagg gctttgaggt gtgtactctt tttgtgacag tggatgtcgc
     8701 ccccttgttg tcggtcagac tcagcgtcac cgtctgtacg cctcccgccg ggaagcggtg
     8761 ggcaatgacg gaatcgttcc gggcatcggg gggactcccc gcctccagcg tccaggagta
     8821 ccgggtgatg atcccgtccg gatcgtacga cggcgaggcg tccagaatgg tctcgggcga
     8881 ccacttcgga tcctgcggcc ggatgcggaa atcggccacc ggagacttgt tgggtccgag
     8941 cgtgatccgc gccacgggcg aagggatatt ccacggatgc gtctccttcg gatgcgtatt
     9001 atcggcgaac tgcaagtcca ccaccgcctc ggcctcgtgc acgcgcacgc gcagcctgac
     9061 gggcttcccc ggcagtcccg ccgcgacgcc ggtgaagagg tcgaacgtgc tcctcttgct
     9121 ataggtcagc gaggaggtaa tatcgttccc ctcctcgtcg aacacccgaa gcccgcgcag
     9181 atggtactcg agaggcgctc tcctgttcag ctccggatcg aagagctcca ggcggaagcc
     9241 gtacatcttc gggacgaaaa aggagtgcgc tatctctccg gaaacggggc cgccgcgtcc
     9301 ggcggttacc gagaggctcc cccgcccctc gacggagtac ccccacaccg agggctcgct
     9361 ctccatccct tcatacagca tgtccacctc ggcggcgccg ctgcgcgtct ccccggcgcg
     9421 catgctccgc ggaaggccgg aaacgccgga gagcttcagg tttgtccccg aaaaggacca
     9481 ggcaagatcg ttcaggtcgt acggcgcggg gttggtcagc tcccagtcgg cgaagagctt
     9541 gatatgcccg ttccggtccg tctgtagccg cttcgttacg gccagctgca gcgggttgcg
     9601 aaggcgacgc agcgtcactt cgaagactct cgataccggg ccgagacgag cctccacagg
     9661 aaccacgacg aattcggcgc ctcctgtcgg aggcgtttcc cagacgtatc gcccgccttc
     9721 gggatccgcg ctgcgcaccg ttccgcctcc gaacgtcacg tcgagctggt ttccgaagct
     9781 gcgtccgtac tcgtgaagga agcgcaccgc gacctcggtt tgctcgaagg tggacatcgg
     9841 agcacccgcg tgcgggtcga gcggcgccat cgcgatgtcg agccacccct gaccggaggc
     9901 atcgaagcag atcaggttgg cgcctcgcag cggagacgat atcgacgggc gcgacgagga
     9961 gaaggagtag acctttccgt cgcgggaaaa actgtccgcg aagtgcatgg tcatcccagg
    10021 agagaagggg tacatgccgg tacagacacc ggtgagggta tccaccttga tgaaggtgta
    10081 ggggaaaaag tcggaaggga agagcatttc gctctgcgag acgaagaagc cgtaccttgt
    10141 tctgcactct gcaagcgcga actccccctc cggcacgccg agcggggagt acctccggat
    10201 acctgcgggc ctcccccaat ctccgaggac gattcccccg tcaggcgcga accagccgga
    10261 aaagtgcctg ctgtttttca cgagcaccgt aaccgcgccg gacggcgtcg tcaaaagata
    10321 tgtgtaggag tcccccgaat acccctgctc cgccagccgt ccgccgcgaa gggagagcag
    10381 atacttcccg tcgatctggt ccagtatttg gagacatgaa ggagaatgga gcttgcagac
    10441 ggagatccct tcctcgacca aatcgaactc cccgaaga
//
LOCUS       DAOPKU010000027        10198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_6904_length_10198_cov_4.921128, whole genome shotgun sequence.
ACCESSION   DAOPKU010000027 DAOPKU010000000
VERSION     DAOPKU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6904_length_10198_cov_4.921128"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..610
                     /locus_tag="PK849_01585"
     CDS             5..610
                     /locus_tag="PK849_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HPE64844.1"
                     /translation="MLVGRVVAEVRKRLSFEVEASGRHVHLSREHVDALFGKGHTLTD
                     SRGLSQPGQFLCSERVTLAGPKGAIENVAILGPERKQSQVELSITDATMLGVKAPVRD
                     SGDLEGSAPLRLRTPKGEIALEKGVIVAQRHIHMTPQDAELFGVADGDRVSVRVAGSR
                     PAILEGVLVRVSPKYSLAMHIDYDEANACGYSKGVRGTIIR"
     gene            638..1465
                     /gene="eutJ"
                     /locus_tag="PK849_01590"
     CDS             638..1465
                     /gene="eutJ"
                     /locus_tag="PK849_01590"
                     /inference="COORDINATES: protein motif:HMM:NF011660.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HPE64845.1"
                     /translation="MHELEKGNGLLDATYARRGKKAAVRPGTMLKVGVDLGTASIVIV
                     VLDGNNTPLTWEMEEASVIRDGLVVDFAGAGAIVRRLKGRVEERLGAELHSAAIAVPP
                     GTGVRDCATHGYVVESAGLEVTSVLDEPTAANAVLGIRDGAIVDIGGGTTGIAVFSEG
                     AVTAVYDEPTGGTHVSLVIAGNRKIPFPDAERLKQDPRMHRELLPLVTPVLQKMASIV
                     SGCVKGHDVKVLYLVGGTCCLEGMERVIERETGIPAFKPAHPFLVTPLGIAMECKSL"
     gene            1618..2292
                     /locus_tag="PK849_01595"
     CDS             1618..2292
                     /locus_tag="PK849_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64846.1"
                     /translation="MDQAQLIDVVTKELIRRLGLTTDAPQEKPLLIAGEPGCCGALEG
                     VCVEYRSSGDETPDWSRYSGAAIPSLSENQLVLASFGLKYGAESSTMVDALLAGVPVY
                     VIEEGVAWRRAACPDSPLGKHYEACEAKLRSFGVRFVPAAKLAGCAAADDVKKAVPAA
                     CVEVPADAPCVDLRSKKVLSERDLQDVCSNGCGEILVAARAIITPLGLDWLRLKGISV
                     RREGTK"
     gene            2289..2564
                     /locus_tag="PK849_01600"
     CDS             2289..2564
                     /locus_tag="PK849_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HPE64847.1"
                     /translation="MIIAKVVGNLWATKKDDKLNGLKFFVVRPAKGSKSETFVAVDGA
                     GAGIGDDVLVSQGSSARTVFAGGDVPVDAVIVGIIDSVEVDESLLEY"
     gene            2701..3825
                     /locus_tag="PK849_01605"
     CDS             2701..3825
                     /locus_tag="PK849_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HPE64848.1"
                     /translation="MGINDIILYIMTGFMVLGALDKAFLNSRLGFGEKFEEGFMAMGS
                     LMLAMGGVITLAPLLAAILKPVIVPLYQAVGADPAMFATTLLANDMGGYPLAMQMAEN
                     PAVGRFAGIILGAMMGPTIVFSIPVALGIIEKKDHPALAKGILLGIVTIPFGCLAGGL
                     VMLSSEFTVGMMLVNLIPVVIFSALIATGLKMIPNAMIKGFTYFGKGVVTVITIGLAM
                     GIIEALSPFRFFAEGSPMALAPLSDSIATIGAIAIVLAGAFPMVMFITKVFKQPLLVF
                     GRKLGMGDVAAAGMVATLANNIPMFTLMKDMDERGKVINVAFAVSAAFVFGDHLGFTA
                     GVERGLILAMIVGKLVGGITAVILACKVTPANAPAPAKKK"
     gene            3890..4333
                     /locus_tag="PK849_01610"
     CDS             3890..4333
                     /locus_tag="PK849_01610"
                     /inference="COORDINATES: protein motif:HMM:NF018008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE64849.1"
                     /translation="MDKKELESLVRKIILEIAGEGCGGSFEKTADPSGILSVSIPTVK
                     PAPFDTGKKGDQVYLTDVVTLQESPRLGCGVMEMEESAFDWTLRYDEVDYIIEGTLEI
                     LIDGRKVTGKKGDMIFIPANSSITFSAPCFARFLYVTYPADWASQ"
     gene            4413..5702
                     /locus_tag="PK849_01615"
     CDS             4413..5702
                     /locus_tag="PK849_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HPE64850.1"
                     /translation="MKKVTNNRYLKCVLPLLLVALLVSAASAAPIGLDPGKWAPATYQ
                     RLAAFVEANGSGGPNYDPAKPPYVVFDWDQTCIFNDTEEALFRYKIETLNFKMTPEVF
                     AEAIRKDIPKDNFSDDYKNADGKTVNIDLIGADLDAAYTFLYKNYEGFGAGGKMTLAE
                     ARATEEFIDFRAKLAWLYEAIGGTFSADVSYPWVLYLFSGMTADEVKALAEKSNDAAL
                     ADKLETYTLSSSKTLQSKAGYISMGGYKRGLRTVPEMSNLMNVLRSHGIHVYVCTASL
                     DNVVRVFSNLPKYGYNVPEENVLGMRVAMEDGKYVPRYDDTWVQTQQAGKTEAIKREL
                     VSKYGYGPIAVFGDSQGDYKMCVDFEETQVVLVVNRLRKDDFGKLGLQAMETHGKPDA
                     KFILQGRDENIGAFRPDEKTIKYGKTEPELFHSSLVK"
     gene            5733..6542
                     /locus_tag="PK849_01620"
     CDS             5733..6542
                     /locus_tag="PK849_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HPE64851.1"
                     /translation="MKLITEQDLRAEIGGKDLRSYRVDRGTIVTPSARQYLSDRKIEL
                     LVGDEAPAQPKETIPPATLPPQGAEAAEPKRARFIGPDGGTFTEKPEHLTHLSGNRLV
                     TKGHPRIAFRGRMDSLQSKIMELQVRAVELGNGVLAEELDELLDFARSLLAAEVTGRP
                     VDDVPLLGLELDEIRAMSHNPKKTFGLGHIRPHYSMGALCVGLNALRSEVREVELAAF
                     RAFSGSDGALDSGQDMPDRRLDVLRALNRLSSAFYILMYRHLPEGYDREFK"
     gene            6713..8224
                     /locus_tag="PK849_01625"
     CDS             6713..8224
                     /locus_tag="PK849_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HPE64852.1"
                     /translation="MTETVLSVGIDIGTSTTQLVFSALEIENTAGVTSVPRISVVDKR
                     IVYRSDIHFTPLLSSTVIDAEGVRRIVESEYRAAGMDREKISAGAIIITGETARKENA
                     ETLLRSMEGLAGDFVVATAGSDLESILAGKGSGAAAYSKRELTSVVNFDIGGGTTNVA
                     IFRNGEVTDTTCLDIGGRLVRLDPKTVSRRPANAREASKEPSRSLTANDSVAGRVEYI
                     APGLAPVISSLGLPLAEGKTASVDDLERLSRRMAAFFDELMGLAPRSSLFESMLTAHD
                     FRSGDLKVKHVSFSGGVADGIAGVPDGGPFVYGDIGILLGQAVKWTETYRNLDVVQAE
                     ETIRATVVGAGSHSLEISGSTIGFSDESVFPLKNLPILKLTEEDERNSLGRALSEKLH
                     WFKDSSGRQRTVAVSFRGRENPSFDEVQRIAEDLHDGLSEYLTHSDKVILVVENDMAK
                     ALGYALRSRLGDGKKVVSIDGIKVENGDYIDIGKPLAKGRVLPVVIKTLVFGG"
     gene            8259..9608
                     /locus_tag="PK849_01630"
     CDS             8259..9608
                     /locus_tag="PK849_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPE64853.1"
                     /translation="MSDILEKIRNAGVVGAGGAGFPTHVKFARACEYILLNGAECEPM
                     LEVDQQIASVHTKKLFGAMTALVELTGAKGGIFALKEKYHDAIRNVEAALPAFPKLCH
                     HVLSNSYPAGDEHVLVYEVFKRIVPEGGIPLDVGVVVLNVETLYNIASALEDRPVIEK
                     YLTVAGEVKNPATLKVPVGVSFGEVIEACGGPTVKEWVAVDGGPMMGRLVSDPQEPVS
                     KTTKGILLLPRDHPLIISKGRDMHEMMRLAKIACCHCMLCTDVCPRYLLGHKLRPDKL
                     MRLAAYNSTCEHDAAATEAFLCCECGLCETACIMGLQPWKLNKELKVRMNAAGIKNPH
                     HERPEQVNPFREYRLFPVTKLTHRLGLSKYIGRKAPLREEFPSPVEKVVLKLKQHIGA
                     PTEPIVAPGDVVERGQMVARGEGPVSAHVHSSISGVVRAVEKDRIVVEATTDGRENE"
     gene            9605..10153
                     /locus_tag="PK849_01635"
     CDS             9605..10153
                     /locus_tag="PK849_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HPE64854.1"
                     /translation="MSKAIGLVEYKTIPKGIEAADAMLKASEVQLLFSSPICPGKYVT
                     IVGGEVDSVKTAVDKAVAVGDIFTVDSHVLTSIHPSVLPAITATVEIKKVEALGVVET
                     ISAVAAIQAGDVAAKAGNVQLVEIRIARGLGGKGFVLFTGDLGSVETSVRACEKKLGE
                     EGGIVSSAVIPSPHKDLISSVL"
BASE COUNT         1937 a         3060 c         3462 g         1739 t
ORIGIN      
        1 tgaactgctc gtcgggcggg tggtcgccga ggtccgaaag cgcctctcct tcgaggtgga
       61 ggcctcaggt cggcatgtcc acttgagccg ggagcatgtc gacgcgctct tcgggaaggg
      121 gcataccctc accgactccc gcgggctttc gcagccgggg cagttcctct gctccgagcg
      181 cgtgacgctg gccgggccga agggcgcgat tgaaaacgta gcgatactcg gccccgagcg
      241 gaagcagagc caggtggagc tttccattac ggacgcgacg atgctcgggg tgaaagcccc
      301 cgtccgcgac tccggggacc tggaggggag cgcgccgctt cggctgcgga ctccgaaggg
      361 ggagatcgcg ctcgaaaagg gcgtgatcgt ggcccagcgg catatccaca tgacgccgca
      421 ggacgcggag cttttcggcg tggccgacgg agaccgcgtg agcgtccggg tggcgggaag
      481 ccgtcccgcg atcctggagg gcgtgctggt gcgcgtgtcg ccgaagtaca gcctggcgat
      541 gcacatcgac tacgacgagg cgaacgcctg cggctactca aagggcgtcc gcgggacgat
      601 catccgctga acagggaagc aagttaggag agggaacgtg cacgaacttg aaaaagggaa
      661 cgggctgctg gatgcaacct atgcgcggcg cggaaagaag gccgccgtgc gcccggggac
      721 gatgctgaag gttggcgtcg acctgggaac cgcctccatc gtcatcgtcg tgctcgacgg
      781 gaacaacact cccctgacgt gggagatgga ggaggcgtcg gtcatccgcg acgggctcgt
      841 ggttgacttc gccggggcgg gagccatcgt ccgccgtctg aagggacggg tcgaggagcg
      901 cttgggcgcg gagctgcaca gcgcggccat cgccgtcccc cccggcacgg gagtcagaga
      961 ctgcgcgacc cacggctacg tggtggagag cgcggggttg gaggtcacat cggttctgga
     1021 cgagccgacc gccgcgaacg cggtcctggg aatccgcgac ggcgcgatcg tggacatcgg
     1081 aggcggaacc accggcatcg ccgtcttctc ggaaggcgct gtgacggccg tctatgacga
     1141 gccgaccggc gggacgcacg tttcgctggt gatcgcgggc aaccggaaga taccttttcc
     1201 ggacgcggag cggctgaagc aggatccgcg gatgcacagg gagctgctgc cgctggtgac
     1261 gccggtgctt cagaagatgg cgtccatcgt gagcggctgt gtgaaggggc acgatgtgaa
     1321 ggtgctctat ctggtcggcg gcacgtgctg cctggagggg atggagcgcg tgatcgagag
     1381 ggagacggga atccccgcct tcaaaccggc gcacccgttc ctggtaaccc cattaggtat
     1441 agcgatggag tgcaagagct tataaggaat gcggccagaa gcatccctac gcgcctacgg
     1501 cgctgggagg ctacgctctg ccgcataagc gaccatcagc gcgaagcctc cggcatgttt
     1561 ttgccggaca attcgcaaaa gcggcgaatt ggatgtctgc ttaagggtgg tgattagatg
     1621 gatcaggcgc aattgattga tgtggtgacg aaggaattga tacggcggct cgggctgacg
     1681 acggatgctc cgcaggagaa gccgctgctg atcgcgggcg aacccggatg ctgcggcgcg
     1741 ctcgaaggcg tgtgcgtcga gtaccgctct tcgggcgatg aaacgccgga ctggagccgc
     1801 tactccgggg cggcgatccc gtcgctttcg gagaaccagc tcgttcttgc ctccttcggc
     1861 ctgaagtacg gcgccgagtc ctcgacgatg gtggacgcgc ttcttgcagg cgttcccgtg
     1921 tacgtgatcg aggagggggt ggcatggcgg cgcgccgcgt gccccgattc tcccttgggg
     1981 aagcattacg aggcgtgcga ggcgaagctg cgctcgttcg gggtgcggtt cgtcccggcg
     2041 gcaaaactcg ccggatgcgc ggcggcggat gacgtgaaga aggcggtccc ggcggcgtgc
     2101 gtcgaggttc cggccgacgc gccgtgcgtg gatctgcgat cgaagaaggt gctctcggag
     2161 cgcgatcttc aggatgtgtg ttcgaacggc tgcggtgaaa tacttgtggc cgcgagggcg
     2221 atcatcacgc ccctcgggct ggactggctg cgcctcaagg gaatctccgt ccgccgggag
     2281 ggaacgaagt gattattgcg aaggtcgtgg gcaacctctg ggccacgaaa aaggatgaca
     2341 agctcaacgg cctgaagttc ttcgtggtcc gtccggcgaa ggggtcgaaa tcggagacct
     2401 tcgtcgcggt ggacggcgcc ggagcgggca tcggcgacga cgtgctcgtc tcgcagggca
     2461 gctcggcgcg gaccgtcttc gcgggcggcg acgttcctgt ggacgccgtc atcgtcggca
     2521 tcatcgactc cgtggaggtg gacgagagtc tgctggagta ttagcggctc attgcgcccg
     2581 agacacaccg ggcagggtat cgccgcgccg ctcgaaatcc gaggggcgcg aggatgcacc
     2641 atcggcactt tgagtaccgg aaaagaaccg gaaccgccat tctattcgga ggtgaactgc
     2701 atgggaatca acgatatcat tctctacatc atgaccggat tcatggtttt gggcgcgctc
     2761 gacaaggcgt ttctgaacag ccgtctcggc ttcggcgaga agttcgagga gggcttcatg
     2821 gcgatgggct ccctgatgct cgcgatgggc ggcgtcatca cccttgctcc gctgctcgcc
     2881 gctatcctga agcctgttat cgtgccgctc taccaggccg tgggcgcgga tccggcgatg
     2941 ttcgccacca cgctgctcgc caacgacatg ggcggctatc cgctcgcgat gcagatggcg
     3001 gaaaacccgg ccgtcgggcg cttcgcaggc atcatcctcg gcgccatgat gggcccgacc
     3061 atcgtcttct ccatccccgt ggctcttggc atcatcgaga agaaggacca cccggcgctc
     3121 gccaagggaa tcctgctcgg tattgtgacc atccccttcg gctgcctcgc gggcggcctc
     3181 gtcatgctga gcagcgagtt caccgtcggt atgatgctcg tcaaccttat tcccgtggtc
     3241 atcttctccg cgctcatcgc gacggggctc aagatgattc cgaacgcgat gatcaaaggg
     3301 ttcacctact tcggcaaggg cgtcgtcacg gtcatcacca tcggattggc gatgggcatc
     3361 atcgaagcgc tttctccgtt ccgcttcttc gccgaaggat cgccgatggc gcttgctcct
     3421 ctctccgatt ccatcgcgac aatcggcgct atcgccatcg tgctcgcagg cgcgttcccg
     3481 atggtcatgt tcatcaccaa ggtcttcaag cagcctctcc tggtcttcgg caggaagctc
     3541 ggcatgggcg acgtggcggc ggcgggaatg gtggcgacgc tggcgaacaa catcccgatg
     3601 ttcaccctga tgaaggatat ggacgagcgc ggtaaggtca tcaacgtcgc cttcgcggtg
     3661 agcgcggcct tcgtcttcgg cgaccacctc ggcttcacgg cgggcgtcga gcgcggcctc
     3721 atcctcgcca tgatcgtcgg caagctggtc ggcggcatca cggctgtcat ccttgcgtgc
     3781 aaggtgacgc cggccaacgc cccggccccg gccaaaaaga agtagtgcgc ttcctccgga
     3841 gggcggccgc cctccggagg accgttcgta gaaagcgtgg tgtgtgtgca tggacaaaaa
     3901 agagcttgaa tcgctcgttc ggaagatcat tctggagatc gcgggcgaag ggtgcggggg
     3961 ctccttcgag aagacggcgg acccgagcgg catcctctcg gtgagcatcc ccaccgtgaa
     4021 gcccgcgccg ttcgacacgg gcaagaaagg ggaccaggtc tacctcacgg acgtggtcac
     4081 gttgcaggag agcccccgcc tggggtgcgg cgtcatggag atggaggagt cggccttcga
     4141 ctggacgctt cgttacgacg aggtggacta catcatcgaa ggaaccctgg agattctgat
     4201 cgacgggcgg aaagtcacgg gcaagaaggg cgacatgatc ttcatccccg cgaactcgtc
     4261 gatcaccttc agcgcgccct gcttcgcgcg ttttctctac gtgacctacc cggcggactg
     4321 ggcgtcgcag taaccggcgg caagcggaat tccattcgga agagggaggt gtgtcaccac
     4381 gctccccgga ggtatgaagg agggacagac acatgaaaaa agtcacgaac aacaggtatt
     4441 tgaaatgcgt tctgccgctt ctccttgtcg cgcttctcgt ctccgcggca tccgccgccc
     4501 cgatcgggct cgatcccggc aagtgggccc cggcgaccta ccagcgtctc gccgcgttcg
     4561 tcgaggccaa cggctccggc ggtccgaact acgacccggc gaaaccgccc tatgttgtct
     4621 tcgactggga tcagacgtgc atctttaacg ataccgagga ggcgctcttc cggtacaaga
     4681 ttgaaacgct caacttcaag atgacgccgg aggttttcgc cgaggcaatc cggaaggaca
     4741 tcccgaagga caacttctcc gacgactaca agaacgccga cgggaagacc gtgaacatcg
     4801 acctgatcgg cgccgatctc gacgcggcct acactttcct ctacaagaac tacgaggggt
     4861 tcggcgcggg cgggaagatg acgctcgccg aggcgcgggc caccgaggag ttcattgact
     4921 tccgcgccaa gctcgcgtgg ctgtacgagg ccatcggcgg cacgttcagc gcggacgtga
     4981 gctatccgtg ggtgctctac ctcttcagcg ggatgactgc ggacgaggtg aaggcgctcg
     5041 ccgagaagtc caacgacgcg gcgctcgccg acaagctgga gacctacacg ctctccagct
     5101 ccaagacgct gcagagcaag gcggggtata tcagcatggg cggctacaag cgcggcctgc
     5161 ggacggttcc ggagatgtcc aacctgatga acgtgctccg ttcgcacggc attcacgtgt
     5221 acgtctgcac cgcgtcgctc gacaatgtgg tccgcgtctt ttccaacctg ccgaagtacg
     5281 gctacaacgt tcccgaggag aacgtgctcg gcatgcgcgt ggccatggag gacgggaagt
     5341 acgtcccgcg ctacgacgat acgtgggtgc agacgcagca ggcggggaag accgaggcca
     5401 tcaagcgcga acttgtgagc aagtacggct acggtcccat cgccgtcttc ggcgacagcc
     5461 agggagacta caagatgtgc gtggacttcg aggagacgca ggtcgtcctc gtggtgaacc
     5521 gcctccgcaa ggacgacttc ggcaagcttg gcctgcaggc gatggaaacg cacggaaagc
     5581 ccgacgcgaa gttcattctt caggggcgcg acgagaacat cggcgctttc cgccccgacg
     5641 aaaagacgat aaaatacggc aagacggagc cggagctctt tcactcgagt ctggtgaagt
     5701 agcgcacggc cggatgccgg ggggtgactg gaatgaagct gatcacagaa caggatctgc
     5761 gggccgagat aggcggcaag gatcttcgct cctaccgggt tgaccggggg acgatcgtca
     5821 ccccttcggc tcgccagtac ctcagcgacc ggaagataga gctcctggta ggggacgagg
     5881 cgccggcgca gccgaaagag acgatacctc cggcgaccct cccccctcag ggggcggagg
     5941 ccgcggagcc gaagagggcc cgtttcatcg gcccggacgg aggaaccttt accgagaagc
     6001 cggagcacct gacgcacctc tcgggcaacc ggctggtgac caaggggcac ccccgcatcg
     6061 cgttcagggg gaggatggac agcctccagt cgaagatcat ggaattgcag gtgcgcgccg
     6121 tcgagctggg caacggcgtg ctcgccgagg aactggacga actgctcgac tttgcgcgct
     6181 cgctccttgc cgccgaggtg acggggcgtc cggtggacga tgtgccgctt ctcgggctgg
     6241 agcttgacga aatcagggcg atgtcccaca atccgaaaaa gaccttcgga ctggggcaca
     6301 tccgtccgca ctactcgatg ggcgcgctct gcgtcgggct gaacgccctg cgctcggagg
     6361 tccgcgaggt ggagctggcg gcattccgcg ccttctccgg aagcgacggc gcgctcgact
     6421 ccggccaaga catgccggac aggcgtctgg acgtcctgcg cgccctcaac cgcctcagca
     6481 gcgccttcta catcctgatg tacaggcatc tgccggaggg gtacgacaga gagtttaagt
     6541 aggcgaccaa cgtaagcagg cgaccaacga gctgattttg cgagttgcgt cgatcgctcg
     6601 cgtactcgca gcgcagcgga gggtatagtg cgccgtgctc ttgggcgcac tctcagggat
     6661 tgagaaaaac attggtgatc acttgttgta aaagatttgg ggtgttgttt tcgtgactga
     6721 aaccgtgctc agcgtcggca tcgacatcgg aacatcgaca acgcagcttg tcttcagcgc
     6781 gctcgaaata gagaacacgg ccggggttac ttccgtgccg cgcatctccg tcgtggacaa
     6841 gcggatcgtc taccggagcg acatccactt cacgccgctg ctctcctcca ccgtgatcga
     6901 cgcggaagga gtgcggcgga tcgtggagtc ggaataccgc gccgcaggca tggacagaga
     6961 gaagatctcc gccggagcga tcatcatcac cggcgagacg gcgcggaagg agaacgccga
     7021 gacgctgctg cggagcatgg aggggcttgc gggcgacttc gtcgtcgcca ccgcgggttc
     7081 cgacctggag tccatcctcg cggggaaggg ctcgggagcg gcagcctact cgaagcgcga
     7141 actgacctcc gtggtcaact tcgacatcgg cggcggcacg acgaacgtcg ccatcttccg
     7201 caacggcgag gtgaccgaca ccacctgcct cgacatcggc ggcaggctcg tgcgcctcga
     7261 cccgaagact gtaagtagac gaccggcgaa cgcgagagaa gcgagcaagg agccgagtcg
     7321 gtcacttacg gcgaatgatt ctgtcgccgg aagagtggag tacatcgcgc cggggctcgc
     7381 cccggtgatc tcctcgctgg gccttcctct ggccgaggga aaaaccgcgt cggtggacga
     7441 tttggagcgc ctttccaggc gcatggccgc cttcttcgac gagctgatgg ggctcgcccc
     7501 gcgaagttcg ctttttgaga gcatgctcac cgcgcacgac ttccggagcg gcgacctgaa
     7561 ggtaaaacac gtctcctttt cggggggcgt cgccgacggc atcgccggcg ttccggacgg
     7621 tggcccgttc gtctacggcg atatcggcat cctgctcggg caggcggtga agtggacgga
     7681 gacctaccgg aatctcgacg tcgttcaggc ggaggagacc atccgcgcga cggtggtggg
     7741 ggcggggagc cactcgctgg agatcagcgg gagcaccatc ggcttctcgg acgaatcggt
     7801 ctttccgctg aaaaacctcc ccatcctgaa gctcacggag gaggacgagc ggaacagcct
     7861 cggccgcgcg ctttcggaga agctccactg gttcaaggac tcctccggac ggcagcggac
     7921 ggtcgccgtc tccttccggg ggagggagaa cccctccttc gacgaggtgc agcggatcgc
     7981 ggaagacctg cacgacggac tgtcggagta tctgacgcac tcggacaagg tgatcctcgt
     8041 cgtcgagaac gacatggcga aggcgctggg gtacgcactc cgcagccgcc tcggggacgg
     8101 gaagaaggtc gtgagcatcg acggcatcaa ggtggagaac ggcgactata tcgacatagg
     8161 caaaccgttg gcgaagggga gggtgcttcc cgtggtgatc aagaccctcg tcttcggagg
     8221 atgaacggcc cggaacgggg aacaggggga ggggctcgat gagcgacata cttgagaaaa
     8281 tacggaacgc gggggtcgtc ggcgcgggcg gagcgggatt tcccacccac gtcaagttcg
     8341 cccgcgcctg cgaatacata ctgttgaacg gcgcggagtg cgagccgatg ctggaggtgg
     8401 atcagcagat cgcctcggtg cacacgaaga agcttttcgg cgcgatgacc gcgctggtgg
     8461 agctgacggg ggccaaggga ggcatcttcg ccctcaagga gaagtaccac gacgccatcc
     8521 gcaacgtgga ggcggcgctt ccggcctttc cgaagctctg tcatcacgtg ctctcaaaca
     8581 gctatccggc gggcgacgag catgtgctcg tctacgaggt cttcaagcgc atcgtccccg
     8641 aggggggcat tccgctcgac gtgggcgtgg tcgtgctcaa cgtggagacg ctctacaaca
     8701 tcgcttcggc gctggaggac cgtcccgtga tcgagaagta cctgacggtg gcgggcgagg
     8761 tgaagaatcc ggcgacgctc aaggttcccg tcggcgtctc gttcggcgag gtcatcgagg
     8821 cgtgcggcgg cccgacggtg aaggagtggg tcgccgtcga cggcggcccg atgatgggcc
     8881 gtctggtgtc cgatccgcag gagcccgtga gcaagacgac gaagggcatt ctgctgcttc
     8941 cccgcgatca cccgctgata atctcgaagg ggcgcgacat gcacgagatg atgcgtctgg
     9001 cgaagatcgc ctgctgccac tgtatgctct gcacggacgt ctgtccccgc tatctgctgg
     9061 ggcacaagct ccgcccggac aagctgatgc gcctggccgc ctacaacagc acctgcgagc
     9121 acgacgccgc ggcgacggag gcgttcctct gctgcgagtg cggcctctgc gagacggcgt
     9181 gcatcatggg gctccagccc tggaagctga acaaggagct gaaggtccgc atgaacgcgg
     9241 cggggatcaa gaacccgcac cacgagcgcc ccgagcaggt caacccgttc agggagtacc
     9301 gcctcttccc ggtgacgaag ctgacgcacc gcttgggact gtcgaagtac atcggacgga
     9361 aggccccctt gcgggaggag ttcccctctc cggtcgaaaa agtcgtgctg aagctgaaac
     9421 agcacatcgg cgcgcccacg gaacccatcg tggcccccgg cgacgtcgtg gaacgcggcc
     9481 agatggtcgc gcggggagag gggcccgttt cggcgcacgt gcactcgtcg atctccggcg
     9541 tcgtgcgcgc ggtcgagaag gacagaatcg tcgtcgaggc aacgaccgac gggagggaga
     9601 atgaatgagc aaagccatag gactcgttga atacaagacc ataccgaagg gtatcgaggc
     9661 ggcggacgcc atgctgaagg cttcggaggt gcagctgctc ttctcgtcgc ccatctgccc
     9721 cggcaagtac gtcaccatcg tcggcggcga ggtggactcc gtaaaaaccg ccgtggacaa
     9781 ggccgtcgcg gtcggcgaca tcttcacggt ggactcgcac gtgctcacca gcatccaccc
     9841 gtcggttctt cccgccatca cggccaccgt cgagattaaa aaggtggagg cgctcggcgt
     9901 ggtcgagacc atctccgcag tcgccgcgat tcaggcgggc gacgtggcgg cgaaggccgg
     9961 caacgtccag ctcgtggaaa tccgcatcgc gcgcggcctg ggcgggaagg ggttcgtcct
    10021 cttcaccggc gacctgggaa gcgtggaaac atcggtgcgc gcctgcgaga agaagctcgg
    10081 cgaagagggc ggcatcgtca gctccgccgt catcccctcc ccgcacaagg acctgatctc
    10141 ctcggtgctg taaaactgtt ttcccgcaca catcacgaag acgtgtgcgg gaaaattt
//
LOCUS       DAOPKU010000028        10147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_6944_length_10147_cov_4.679053, whole genome shotgun sequence.
ACCESSION   DAOPKU010000028 DAOPKU010000000
VERSION     DAOPKU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10147
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6944_length_10147_cov_4.679053"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PK849_01640"
     CDS             complement(<1..825)
                     /locus_tag="PK849_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64855.1"
                     /translation="MNILFCSDAMIVDGVTSFVFHLATALSEGGHRVAVLGRWGGKGF
                     QSRLRTYGVEVISIPSVTVGNFWFDKKAREFAPDVLVTDSRRSFPLAVRLKRATGAKI
                     FTFFLDNLEKTDKKGRDVDSLVRWSDAWLSAEPPLLEKLEEIPTPFPKFLFQRPLTGL
                     LTPTPIPSRDPFRVLCFGRISGYKSAGPLKLLQEAPTLKKAIPSLKIAFVGGGWRTWK
                     FRLMAARANAAAGERYLRIIGTQTDPQRWFEWATLVCAGSTSAVEAVLAHRPTVVFS"
     gene            complement(871..2136)
                     /locus_tag="PK849_01645"
     CDS             complement(871..2136)
                     /locus_tag="PK849_01645"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HPE64856.1"
                     /translation="MSPGEAKEAFSHDTQDVPQQRPAHGRASGPLLVFILSDLIRGHL
                     SQSRGIAGWLSRFTGADIVEIDVPRITGKRRVELLKVKGRRLPKMNSCDILVWLDETG
                     GSVLFDTMKNELAARKLTGKDALFLAAGGSAAPFALALAKMTRGKSCVLMTPSVLGTA
                     PFDFAVVPAHDRPRLTPNLLATLGAPNAIFPDELERKGWELAELYPPVESAEQRWGLL
                     VGGDDANYAVTADWVRRVISPILSAAGKEGADLYITTSRRTTPEAEEALSRLAAGRSH
                     VRMLLLASRDEFNPVAGMLGLCSRLFVTEDSVSMVSEAITAGREVFLLRTDRKGKLRV
                     AMQDLTASLVRRRLLPPGVLWGKPRFDALFDVLERRGFLKEMQPRALRRSSSGGASLH
                     ADGPVLNEARRAAEWIVERWGEAGKDNRK"
     gene            complement(2141..2998)
                     /gene="kdsB"
                     /locus_tag="PK849_01650"
     CDS             complement(2141..2998)
                     /gene="kdsB"
                     /locus_tag="PK849_01650"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPE64857.1"
                     /translation="MNILGVIPARYASTRLPGKPLADICGKTLIRRVYERAALSPLLD
                     RLVVATDDERILEEVRSFGGEAVLTSSDHPNGTCRVAEAAEKYEGDIVLNIQGDEPLL
                     DPLMVDEVAQALLDDDSALSSTLCVPITERSMLDDPSVVKVVRDLRGFALYFSRYPIP
                     YIRSDADAVAVYEHIGIYGFRRDFLNIYVSLPPTPLSLAESLEQLKIMEHGYSMKVSV
                     TGAERKGTSVDTPEDLEAVRRILCSNEVKSTVCSNEVKSIVCSNEAKSTVCSNEAKGP
                     AARPAVKGV"
     gene            complement(2995..4242)
                     /locus_tag="PK849_01655"
     CDS             complement(2995..4242)
                     /locus_tag="PK849_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64858.1"
                     /translation="MTMNEQLLKEFYSGLDDRSKESLDAAAEKIVEAKRRGGKVVVAT
                     GSGPNIHEGVTTLIAELMEKGLVDGVTTSSAVVGHEMGGALDLVKMCSASALGFEERF
                     MPRGDVFEFTQLDEERMNALRAEMVLDEELLAKGERAEGRLVIKAAGNMAYPMGLRNE
                     NLAGEILVLCKALGLPFETVAGWGADRRTMLGAGAALGLPVLVSIPQMVGSGHIGMAI
                     GDSISVFERSRRIAAMLESADVIIESAVALTQEIHDGPFECYTGHGIWSWWKGLPVYS
                     LRDKTLIRIDLDENLRKARDLETGGSLIQEAINQGLPKTKISKIPFRMEMSAFARHEG
                     SIPVIGDIGQVWPVLAWKVAGALGISLDFMSYAQHTTEGKAMREWIVREVQPVDREKI
                     LARARKCGISAAPFGNVETVAKP"
     gene            complement(4239..5474)
                     /locus_tag="PK849_01660"
     CDS             complement(4239..5474)
                     /locus_tag="PK849_01660"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPE64859.1"
                     /translation="MDERRGVYSEETRRAFSGGPLWIHAVSVGEAQSAWPLLDALRPA
                     GGEKNLFPVLLSTTTTTGKEMAFRLADSLFERHIYYPWDIPRIVNRALDAIRPRGYAT
                     METEIWPNMLFSLARRGVPAFLVNGRFSERTARTMRKHPSFWRMVYDCFTLLLVRSET
                     DKEMLLSLGVPAERVRVTGDCKVDALLLRRRNACLGPLEALFGVRPGDPPHDDAKRRP
                     ERDPLFLAGSTHEGEEAIVIDAFMHVRRVFPSARLVIAPRHPERADGVRALASEVAPS
                     ALLSDVERQTASSRPDIVVVDRIGVLFDLYGVVDAAFIGGSLVDKGGQNILEPAAFGT
                     PLSHGPYMRDFVEAAKDLGALGIATRVGDASEIAAHWEASMKPERRAAVRESAERYFR
                     ASGGAARLSADEINGVILR"
     gene            complement(5587..6642)
                     /locus_tag="PK849_01665"
     CDS             complement(5587..6642)
                     /locus_tag="PK849_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPE64860.1"
                     /translation="MFCLPWEREGRCLSDVELLETGRGILHAEAAEIERAAQRTGLEL
                     VKAARIVHSCTGRLVVVGMGKSGIIGRKIAATLASLGTPSFFLHAAEGSHGDLGMVCR
                     DDAALFISNSGTTAEVVSLLPHFKRLGAPIIAITGGLNSPLAKNADAVLDSSVRSEAG
                     ITACATPLEPGERDQDALNLAPLCSTTLQLALGDALAGMVTELRGLRPEDFALFHPGG
                     ALGRRLLTRVGDVMGTGEKLPLVGMNVSVSDALFEMTSKGYGATVIVDEQGEIAGVFT
                     DGDLRRLIGSRGVECLSLEVGSVMTRNPLIIQPDKLAVEAVRVMERREISAIIVVEGK
                     RPVGILHLHELLQAGVA"
     gene            complement(6644..7531)
                     /gene="kdsA"
                     /locus_tag="PK849_01670"
     CDS             complement(6644..7531)
                     /gene="kdsA"
                     /locus_tag="PK849_01670"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPE64861.1"
                     /translation="MADTSPEKLWDFSIGRGELTLIAGPCSLETPELGLEIAETVRDI
                     CASLGVRYIFKASFDKANRTSLSGFRGPGLDAGLEQLAEIRRKASVPVLTDIHESWQA
                     EPAARAVDVLQIPAFLCRQTDLLLAAAGTGKPLNVKKAQFLAPGDMKHVVEKCRQGGC
                     SGLMLCERGTFMGYGQLVVDMRSLPLMRALGCPVVFDATHSVQMPGAAGGASGGDRRF
                     VLPLARSAVAAGIDALFLETHPRPEKAKSDGPNLVPLGKMRFLLEQLAELHAFVRERF
                     GRLSLEWADEHAAENKGGR"
     gene            complement(7654..10050)
                     /gene="lpxK"
                     /locus_tag="PK849_01675"
     CDS             complement(7654..10050)
                     /gene="lpxK"
                     /locus_tag="PK849_01675"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPE64862.1"
                     /translation="MNGAGGVRRRFSPANVIQSIRSSVGVALGSYLSFARGETRFSPW
                     ILVYPFGLIARMVVAGRNFAFDHGLIRSEEPPLPVVSVGNITLGGTNKTPFVEMLCRM
                     FQEAGVTPGIISRGYGGRTVDPVVITAEEISAGAASEKTLSRYRDLVGDEPLLLASRL
                     PGISVAVSKDRLRDVDVLSERNVQLIVADDAFQHRRMGRDADIVLVDACCPFGNGWIA
                     PAGILRESPSVLARASAVVVTKSDQVEPERLERLVGELSRFVPKERLFFSRISLLNWR
                     RWNGGWKDAAGERPDSVLAFSAIGSPESFRRSLEAGGVDILKEHRFKDHYRYRMEDMA
                     ALEASLEECGASCMVCTEKDVYNLPQEWRPTRDILVPFISTVLDEEARFRECLLEALR
                     PRMVVASNGYGEDSMGVLLARKLKERFPSASVSAFPIVGRGEHYLKEGIPIDSVPSDS
                     PSGGVIKYRFADLWRDLRSGLLRSIARQMGAWKLLRGRIRTPLCVGDVYLLLHALFGQ
                     GQLPVLIATAKTVYLSGHWRLERFLIKRRSRMAWTRDRDTAEELRRSGVQARFDGNPI
                     MDITCDNTIEPVSWGSENAPRILLLPGSRRRAYDDLVLLLQAVERIHAMLSEGASYLM
                     VVAPTLDTEKLLKACERVPATEEGQWTSFGGEHAPGVRKGTCKIRFFFGPLPAVAGRA
                     HLLVGLGGTANQVCAGMGVPVVSIEEKGKFVQKKLLGDSEVLVPPQPQALAEAAVRIL
                     SDEPLRLRMAAEGMARLGGPGALDKVVEYAASKMGWDLRVRLYETLAGCWGASDGRRP
                     "
BASE COUNT         1902 a         3377 c         3093 g         1775 t
ORIGIN      
        1 ggagaagacc acggtcgggc gatgcgccag cactgcttcg accgccgacg tggagccggc
       61 gcagacgagc gtcgcccatt cgaaccagcg ctgagggtcg gtctgcgttc cgatgatccg
      121 aaggtaccgc tcccccgccg cagcgttggc gcgtgcggcc atcaggcgga acttccacgt
      181 cctccaccct ccgcctacga aggcgatctt gagcgaaggg atagcttttt tcaacgtcgg
      241 cgcctcctgg agcagcttta aaggcccggc cgacttgtac ccgcttattc tgccgaaaca
      301 gaggacccgg aacgggtcgc gcgaaggaat cggcgtcggg gtaagcagac cggtgagagg
      361 gcgctggaag aggaacttcg ggaacggcgt cggtatctcc tccagcttct ccagaagcgg
      421 cggctcggcg gagagccacg cgtcgctcca gcgcaccagg gaatcgacgt cgcgcccctt
      481 cttgtccgtt ttttccaggt tgtccagaaa gaaggtgaaa attttcgccc ccgtcgctcg
      541 tttgagccgc acggcaaggg gaaaggagcg tcgggaatcg gtgaccagaa catcaggcgc
      601 aaactcacga gccttcttat cgaaccagaa gtttccgact gtcacggagg gaatggagat
      661 cacctccacg ccgtacgtgc ggaggcggga ttgaaacccc ttgccgcccc accgtccgag
      721 aacggcgacc cgatgccctc cttcgctgag ggcagtcgca agatgaaaaa caaacgaagt
      781 gacgccgtcg acgatcatgg cgtccgaaca aaaaagtatg ttcatccgag aatggagctc
      841 cttttatcgg cgcgtacaca cgccgcacgt ttattttctg ttgtcttttc ccgcttctcc
      901 ccaccgttcg acgatccact ccgccgcccg ccgcgcctcg ttgaggacgg ggccgtcggc
      961 gtggagcgac gcgcctcccg aggaagagcg tcgaagggcg cggggctgca tctccttcag
     1021 aaatccacgg cgttccagta cgtcgaagag ggcgtcgaag cgcggtttgc cccaaagcac
     1081 ccccggcggc agcagtcgcc tccgtacgag cgacgccgtg agatcctgca tcgcgacgcg
     1141 gagcttcccc ttccggtctg ttcgcagaag gaagacctct cttccggcgg tgatggcctc
     1201 ggaaaccatc gagacggagt cctcggtgac gaaaaggcgc gagcagagcc cgagcattcc
     1261 ggcgaccggg ttgaactcgt cccgcgacgc gagaagaagc atgcgtacgt gcgagcgtcc
     1321 ggcggcaagc ctcgacagcg cttcttccgc ctcgggggtg gtccgtcgcg acgtagtgat
     1381 gtagagatcg gcgccttctt ttcctgcggc cgagagaatc ggggagatca cgcgccgcac
     1441 ccagtccgcc gtgacggcgt agttggcgtc gtcccctccg acgaggagac cccatcgctg
     1501 ctcagcgctc tccacgggag ggtagagctc cgcaagttcc caccccttcc gctccagttc
     1561 atcgggaaaa atcgcattgg gcgcccccag ggtcgccagc aagttaggcg tgagccgcgg
     1621 gcggtcgtgc gcgggcacca ccgcgaagtc gaagggcgcc gtaccgagca ccgagggggt
     1681 catcagaacg cacgacttgc cccgcgtcat cttcgcgagc gcgagggcaa acggcgccgc
     1741 gctcccaccc gcagcaagga agagcgcgtc cttgccggtg agcttgcggg cggcgagttc
     1801 gtttttcatc gtgtcgaaga gcacggagcc tcccgtctcg tcgagccaga cgaggatatc
     1861 gcacgagttc atcttcggca gacggcgccc cttcaccttg agcagctcca cccgccgttt
     1921 tccggtgatc ctgggaacgt cgatctcgac gatgtcggcg ccggtgaagc gcgagagcca
     1981 tcctgcgata ccccggctct gcgagaggtg tccgcgtatc aagtcgctca gaatgaagac
     2041 gagcagaggc ccggacgccc gcccatgcgc cgggcgctgc tgcgggacat cctgggtatc
     2101 gtgggaaaac gcttcctttg cttcgccggg ggacatgacg ctagactcct ttcacggccg
     2161 gccgcgccgc ggggcctttc gcttcgttcg agcacacggt actcttcgct tcgttcgagc
     2221 acacgatact cttcacttcg ttcgagcaca cggtactctt cacttcgttc gagcacaaga
     2281 tacgccggac agcctcaagg tcctccggcg tatccacgct tgtacccttt cgctccgccc
     2341 cggtgaccga aaccttcatg gaatagccgt gctccatgat tttgagctgc tccaaagact
     2401 cggccaacga gagcggcgtc ggggggaggg agacgtagat gtttaaaaag tcccgccgga
     2461 agccgtagat gccgatatgt tcgtacaccg ccacggcgtc cgcgtccgaa cgaatatacg
     2521 ggatgggata cctgctgaag tagagcgcga atccgcgaag atcccgcacc accttcacta
     2581 cggatggatc gtccagcatc gagcgttccg ttataggaac gcagagcgtc gaggagagcg
     2641 ccgaatcgtc gtccagaagc gcttgcgcga cttcatccac cataagcgga tcgagcagcg
     2701 gctcgtcccc ctggatattc agtacgatat cgccctcgta cttctccgcc gcctcggcga
     2761 cccggcatgt cccgttgggg tggtcggacg aggtgagcac ggcctccccg ccgaacgagc
     2821 ggacctcttc gaggatccgt tcgtcgtctg tggccacgac aagacggtcg agcagcggcg
     2881 aaagcgcggc gcgttcgtag actctccgga tgagcgtctt cccgcatata tccgccagcg
     2941 gtttgccggg aaggcgcgtc gaggcgtagc gggcggggat cacgccgaga atgttcatgg
     3001 cttggcgaca gtctccacgt tcccgaacgg cgcggcggaa atgccgcact tccgcgcgcg
     3061 ggcaagaatc ttctcccggt cgacgggctg cacttcccgt acgatccatt cgcgcatcgc
     3121 cttcccctcc gtggtgtgct gggcatagct catgaaatcg agcgagatgc cgagagcgcc
     3181 cgcaactttc cacgcgagga ccggccagac ctgcccgatg tcgccgatga cggggatgct
     3241 cccctcgtgg cgcgcgaagg cggacatctc catcctgaaa ggaatcttgg agatcttcgt
     3301 cttcggcaac ccctggttga tcgcctcctg tatcagcgag ccgcccgtct ccaggtcgcg
     3361 cgccttgcgg aggttctcgt cgaggtcgat acggatgagc gtcttgtcgc gcaaagagta
     3421 gacggggagc cctttccacc acgaccagat gccgtggccg gtgtagcact cgaagggacc
     3481 gtcgtgaatc tcctgcgtga gggcgaccgc cgattcaatg atgacgtccg cggattcgag
     3541 catcgccgcg atgcggcggg aacgctcgaa gaccgagatg ctgtccccga tggccatgcc
     3601 gatgtggccg cttccgacca tctgcgggat gctcaccagt acgggaaggc ccaaagccgc
     3661 ccctgcgccg agcatggtcc gccggtcggc gccccacccc gcgacggtct cgaagggaag
     3721 tcccagcgcc ttgcagagca ccagaatttc tccggcaagg ttctcgttcc gcagccccat
     3781 ggggtatgcc atgttccccg ccgccttgat cacgaggcgt ccctcggcgc gctccccctt
     3841 cgccagcagc tcctcgtcga gaaccatctc ggcgcgaagc gcgttcatcc gctcctcgtc
     3901 gagctgcgtg aattcaaaga cgtcgccgcg gggcatgaaa cgctcctcga acccgagggc
     3961 cgaggcgctg cacatcttca caaggtcgag cgcgccgccc atctcgtggc cgacgaccgc
     4021 agaactcgtg gtcacgccgt cgacgagccc cttctccatc agttcggcga tgagcgtcgt
     4081 taccccttcg tggatgttcg ggccgctgcc cgtcgccacg acgacctttc cgccgcgccg
     4141 cttcgcctcg acgatctttt ccgccgccgc gtcgaggctc tccttggagc ggtcgtcaag
     4201 tccgctgtaa aactccttca gaagctgttc gttcattgtc atcgcagtat cactccgttt
     4261 atctcgtctg ccgagagccg ggcagctcct ccggaagcgc ggaagtaacg ctccgcactc
     4321 tcacggactg ctgcccggcg ctccggtttc atacttgctt cccagtgcgc ggcaatctcc
     4381 gaggcgtccc cgacacgggt tgctataccc agagctccca gatccttcgc cgcctcgacg
     4441 aaatccctca tgtagggccc gtgggagagc ggcgtcccga aggccgccgg ttcgaggatg
     4501 ttctgtccgc ccttgtccac aaggcttccg ccgatgaacg cggcgtccac gacgccgtac
     4561 aggtcgaaga gcacgccgat acggtccacc accacgatat caggtcttga ggatgccgtc
     4621 tgccgctcca catccgaaag caaagcgctc ggcgcaacct ccgaagccag tgcgcgcacg
     4681 ccgtccgccc gctccggatg tctcggcgca atcaccaggc gtgcagaggg aaagacgcgc
     4741 cgaacgtgca taaaggcgtc gatcacgatc gcctcctcgc cttcatgggt gcttccggcg
     4801 agaaagagcg gatcacgctc cgggcgcctc ttcgcgtcgt cgtgcggagg atctcccggc
     4861 cgaacgccga aaagagcttc gagaggcccg agacacgcat ttctcctgcg aagcaagagc
     4921 gcatccacct tgcagtctcc ggtgacacga accctctccg cgggaacgcc gagtgaaaga
     4981 agcatctctt tgtcggtctc cgaacggacg aggagcagcg tgaaacaatc atacaccatt
     5041 ctccagaaag atggatgctt ccgcatggtg cgtgcggtgc gctccgaaaa gcggccgttt
     5101 acgagaaacg ccgggacgcc tctgcgggcg agcgaaaaga gcatgttcgg ccagatctcg
     5161 gtctccatgg tcgcgtaccc cctcggcctg atcgcgtcga gggcgcggtt cacgatgcgc
     5221 gggatatccc acggatagta gatatgccgc tcgaaaagac tgtccgcgag gcggaaggcc
     5281 atctccttgc cggtcgtcgt cgtcgtggag aggaggacgg ggaagagatt cttctcccct
     5341 ccggcggggc gcaacgcgtc cagcagaggc catgcggact gggcctcccc caccgagact
     5401 gcgtgaatcc agagcgggcc tccggagaac gcacggcgcg tctcttccga atagacgcct
     5461 ctgcgctcgt ccagcccctc cgtatacttc ctgagaagcc acgggagcag gagaggatag
     5521 gctccggagg tacagactct gtagaaccct tttcccgctc tccgggtgag cgagtccgta
     5581 cccagctcag gccacccccg cctgaagcag ttcgtgaaga tgcagaatgc cgacggggcg
     5641 cttcccctcc accacgatga tcgcggagat ctcccgccgc tccatcacgc gcaccgcttc
     5701 gacggccagc ttgtccggct ggatgatcag cggatttctg gtcatgacgc tccccacttc
     5761 cagcgagaga cactccacgc ctcttgaacc gatgagccgc cgcaggtcgc cgtcggtgaa
     5821 gacgcccgcg atctctccct gttcgtcgac gatgaccgtc gcgccgtacc ccttgctcgt
     5881 catctcgaag agcgcgtcgc tcaccgagac gttcatgccg acaagcggca gcttctcccc
     5941 cgtccccatg acgtcgccca ctctggtgag caacctgcgg cccagggcgc cccccggatg
     6001 gaagagggcg aagtcctccg gccggaggcc ccgaagctcc gtcaccatcc ctgcgagcgc
     6061 gtcgccgagt gcgagctgca acgtcgtgct gcacaagggg gcgaggttga gcgcgtcctg
     6121 atcgcgttct cccggttcga ggggcgttgc gcacgcggtg atacccgcct cggaacgcac
     6181 cgaggagtcg agcacggcgt cggcgttttt cgccagaggg gagttcagtc ctccggtgat
     6241 ggcgatgatc ggcgcgccga ggcgtttgaa gtgcgggagc agcgagacga cctccgccgt
     6301 ggttccgctg ttgctgatga agagcgccgc gtcgtcgcgg cagaccatgc cgaggtcgcc
     6361 gtgcgatcct tcagcggcgt ggaggaagaa ggagggcgtt cccagggagg cgagcgtcgc
     6421 cgctatcttc ctcccgatga tccccgactt gcccatgccc accaccacga gcctgccggt
     6481 acaggagtgc acgatacgcg cggccttcac cagctccaag cccgttcgct gcgcggccct
     6541 ttctatctcg gccgcctcgg cgtgcagaat tcctctcccc gtttccagga gttcaacgtc
     6601 ggagagacat cgtccctcgc gctcccacgg aaggcagaac atctcagcgt ccccctttgt
     6661 tttcggctgc gtgttcgtcg gcccactcca gggagagacg gccgaatcgt tcgcggacga
     6721 aagcgtgcag ttcggcgagc tgttcgagga gaaagcgcat cttccccagg ggaacgaggt
     6781 tcggaccgtc gctcttcgcc ttctcggggc gggggtgcgt ctccagaaag agcgcgtcga
     6841 taccggcagc cacggcgctc cgggcaaggg ggaggacgaa acgtctgtcg ccgccgctcg
     6901 cgccgcccgc ggcgccgggc atctgaacgc tgtgcgtggc gtcgaagacg acaggacaac
     6961 caagcgcgcg catgaggggc agggagcgca tatccaccac aagctgtccg taccccatga
     7021 aggtgccgcg ctcgcagagc atcaggccgg aacacccgcc ctggcggcac ttctccacca
     7081 cgtgcttcat gtcgcccggc gccaaaaact gcgccttctt gacgttgagc ggcttcccgg
     7141 ttccggcagc ggcgagcagg aggtctgtct ggcggcagag aaacgccgga atctgcagga
     7201 cgtcgacggc gcgcgccgcg ggctcggcct gccacgattc gtgaatatcc gtgagcaccg
     7261 gaaccgacgc cttgcggcgt atctccgcga gctgttcgag cccggcgtcg agccccggac
     7321 cgcggaaacc ggacagggag gtgcggttcg ccttgtcgaa agaggccttg aagatatagc
     7381 gcaccccgag ggacgcgcag atgtcccgca ccgtctcggc gatctcgagt cccagttccg
     7441 gcgtctccag ggagcacgga cctgcgatga gtgtgagttc tcctctccct atcgaaaaat
     7501 cccacagttt ctcgggcgag gtatccgcta tgcggacaac cggctccgaa caaacacccg
     7561 ttccctccgg aacagggatg cgctccgagg cgcatgagga agtattcact gctgcatcat
     7621 ctcctgattc tcaataatca tttcgaacag cggtcaagga cgtcttccgt cggacgcgcc
     7681 ccagcacccc gcgagcgttt catacaggcg gacgcgaagg tcccacccca tcttggaggc
     7741 cgcgtactcc acaaccttgt ccagagctcc cggccccccc agccgcgcca tgccttcggc
     7801 ggccatacgc agccggagcg gctcgtcgga gagaatgcgc accgcagctt ccgcaagcgc
     7861 ctgcggctgc ggggggacaa gcacctcgga atcgccgagg agcttcttct ggacaaactt
     7921 tcccttctcc tcgatggaaa ccactggaac ccccatccct gcgcatacct gatttgcggt
     7981 ccctccgagc ccgacaagaa ggtgcgctct gccggccacc gccggaaggg gaccgaagaa
     8041 aaagcgaatt ttacaggttc ctttccgcac gcccggcgcg tgttctccgc cgaaagaagt
     8101 ccactgtcct tcctccgtcg cgggaacgcg ctcgcaggct ttcagcagtt tttccgtgtc
     8161 gagagtgggc gcgacgacca taaggtagga cgctccttcc gaaagcatag cgtgtatgcg
     8221 ctctacggcc tgcaacagga gaacaagatc gtcatacgcc cgtcgcctgc tcccgggaag
     8281 aagcaggata cgcggcgcgt tctcacttcc ccaggagacc ggctcgatag tattatcaca
     8341 cgtaatgtcc attatgggat taccgtcgaa gcgagcctga acgcctgagc gccgcagctc
     8401 ctcggccgta tcccggtcgc gggtccacgc catccggctc ctgcgtttga tgaggaatcg
     8461 ttcgagcctc cagtgaccgc tgaggtagac agtcttcgcc gtggcgataa gaacaggaag
     8521 ctgtccctgt ccgaagagcg cgtggagcaa caggtagacg tcgccgacgc acaacggggt
     8581 cctgatgcgc ccgcggagca gcttccacgc ccccatctgg cgggctatgg aacgaagaag
     8641 gccggagcgc agatcccgcc agaggtcggc gaaccggtac ttgatcactc cgccgctcgg
     8701 cgagtcggag ggaacggagt cgatcgggat cccctccttc aagtagtgct cgccccttcc
     8761 gacgatgggg aacgccgaaa ccgacgcgga cggaaaacgc tccttgagct tcctggcgag
     8821 cagcacgccc atcgagtcct cgccgtaccc gttggacgcg accaccatcc gggggcgcag
     8881 ggcttccaga aggcactccc tgaatcgcgc ctcctcgtcg agcacggtgg agatgaacgg
     8941 aaccagaata tcacgtgttg ggcgccactc ctgagggaga ttgtagacat ccttttcggt
     9001 acagaccatg cacgacgcgc cgcactcctc caaagaagcc tccagcgcgg ccatgtcctc
     9061 catccggtag cggtagtgat ctttaaagcg gtgttccttc aggatgtcga cgcctccggc
     9121 ttccaaagaa cggcgaaagc tctccggact gccgatggcc gagaacgcga gcaccgagtc
     9181 cggtcgttct ccggccgcgt ctttccaccc cccgttccaa cgcctccagt tgagcagcga
     9241 gatgcgtgag aagaacagcc gttccttcgg gacgaagcga gaaagttccc cgacaagacg
     9301 ctccaggcgc tcgggctcga cctggtccga cttggtgacg acgacggcgg acgccctcgc
     9361 aagcaccgac ggcgattctc tgagaatacc ggccggcgca atccatccgt tgccgaacgg
     9421 gcagcacgcg tcgacaagca cgatgtccgc gtcgcgcccc atccttcggt gctgaaaggc
     9481 gtcgtcggcc acgataagct gtacgttccg ctcggaaagg acatcgacgt cgcgcaggcg
     9541 atctttggag accgcaaccg aaatccccgg aaggcgcgaa gccagaagaa gcggttcgtc
     9601 gccgacaaga tctctgtatc gggagagcgt cttttcggaa gccgccccgg cggatatctc
     9661 ctccgcagtt attactacag gatcaacggt tcgccccccg taccctctgc tgatgatacc
     9721 gggcgtcacg cccgcctcct ggaacatacg gcagagcatc tcgacgaacg gcgtcttgtt
     9781 cgtccctccc agcgtgatgt tcccgacgct caccacagga agcggaggct cctccgagcg
     9841 gatcaggccg tgatcgaaag cgaaattacg accggcgacc accatcctgg caatcaggcc
     9901 gaacggataa acaagaatcc acggggaaaa gcgcgtctct ccgcgggcga aggagaggta
     9961 gcttccgagg gcgacgccga ccgaagagcg gatgctttgg atcacattcg ccggagagaa
    10021 acgccttcgc acgcctcccg cgccgttcat ggagcagcag tctccccgag gacgcacagg
    10081 cggtgataga ggccgttgcg cgcgagcaga tcttcatgcg tcccctgctc ggcgatgcgc
    10141 ccttcgt
//
LOCUS       DAOPKU010000029         9779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_7271_length_9779_cov_4.455574, whole genome shotgun sequence.
ACCESSION   DAOPKU010000029 DAOPKU010000000
VERSION     DAOPKU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7271_length_9779_cov_4.455574"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK849_01680"
     CDS             <1..214
                     /locus_tag="PK849_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE64863.1"
                     /translation="KAFIAAVFGGIGSIQGAMIGGMLLGLIEIMAVAFFPHLSGYRDA
                     FAFVLLIVILLLKPTGLMGEKLEDKI"
     gene            214..1281
                     /locus_tag="PK849_01685"
     CDS             214..1281
                     /locus_tag="PK849_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE64864.1"
                     /translation="MNTRTRNFILNCLLIAALGAFLYWAQNTLDGYKIQVLNLIAVNA
                     ILALSLNLIYGFTGMFSLGHAGFMAIGAYVCSILILSPAQKEAMWILEPLIWPLSVIK
                     APFFVAVLSGGLIAAFFALLIAIPVLRLGGDYLGIATLGFAEIIRVVLTNLSFITNGA
                     LGIKGIPPYANIWWNYAWLLLTLFFIIRLVNSNFGNVLKAIRDDEIAAKNMGINTFFY
                     RVLAFTIGAFFAGVGGALMGSLITTIDPRMFMFVLTFNILMIAVAGGLGSITGSIIGS
                     IVITILLEWLRFVENPLVIGSFEIPGIPGMRMVIFSLALILIILFRREGIMGMREITW
                     DTFFSRGRRSPEHEEKGGKMK"
     gene            1278..2072
                     /locus_tag="PK849_01690"
     CDS             1278..2072
                     /locus_tag="PK849_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64865.1"
                     /translation="MTTPASILRTENLTMRFGGLTAVGNFSIDVPQGKIVGLIGPNGA
                     GKTTVFNMITGFYRPTEGRIFFDGQDITGFQPHMVCRSGISRTFQNIRLFTNETVLEN
                     VMIGCHVRRKSKWWMAPLGLPSFRAEEEAIREKSMDLLKSVGLEDFADEPSASLSYGA
                     QRRLEIARALATDPKFLLLDEPAAGMNPQESQELMSFIRNIQKRFNLTILLIEHDMKV
                     VMGVCEYIWVLDYGVKIAEGNPDAIQSDQKVIVAYLGEEYVKYVEN"
     gene            2059..2787
                     /locus_tag="PK849_01695"
     CDS             2059..2787
                     /locus_tag="PK849_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64866.1"
                     /translation="MLRIENLHVYYGAIHAVKGINLEIPEGAIVTLIGANGAGKSSTI
                     RAVAGLVKNVKGKILYTPSGGDASGQVNLIGKTPEEMVKLGIAMSPEGRRILPHLTVE
                     ENLQLGAFIRNDKDGIERDRDHVYELFPRLKERSWQKGGTLSGGEQQMLAVGRALMSQ
                     PKLVMLDEPSLGLAPLLVKEVFEIIGEINALGKTVLLVEQNAFAALKIASYAYILEVG
                     NIVLEGKGEELLKDARVKEAYLGG"
     gene            2897..3466
                     /gene="pgsA"
                     /locus_tag="PK849_01700"
     CDS             2897..3466
                     /gene="pgsA"
                     /locus_tag="PK849_01700"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPE64867.1"
                     /translation="MGKKVWNIPNTLSLLRVFLAPLVLIFLTFKFTYPVYSVEDLGIL
                     VTVGDVLAGSVFILAALTDTADGYIARKKGMVTNLGKFIDPLADKILVMAALIALVEL
                     HRIPAWIVVVILTREMIVTGVRMVAAAEGIVIAASNAGKLKTVFQIVAIAMIIFNIPF
                     GIHVMWVAMLMTLWSGMDYLIKGKKLFTE"
     gene            3607..6291
                     /gene="secA"
                     /locus_tag="PK849_01705"
     CDS             3607..6291
                     /gene="secA"
                     /locus_tag="PK849_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPE64868.1"
                     /translation="MLEMLKKALGLDPNERALKRYTSVVEQINALEPALLSLSDEELR
                     EKGASFKKRLTEGETPDDMLPEVFAVVREVSRRTIGLRHFDVQLMGGMALHEGKITEM
                     KTGEGKTLVATLAVVLNALSGEGVHLVTVNDYLARRDAEWMGPIYTFLGLSVGVIYAY
                     MDPEERLASYRADITYGTNSEFGFDYLRDNMAVNESHLVQRGHAYCIVDEVDSILIDE
                     ARTPLIISGPSDDNVEMYTTSDRIARQLKEGRDFEKDEKERNVAMTEAGIARCEELLK
                     LPDLFSDAAHSDLAHRIVQALKARILFQKDVHYVVKDGEIIIVDEFTGRLMVGRRYSD
                     GLHQAIEAKEGVKVGRESQTLATITLQNYFRMYRKLAGMTGTAATEAEEFKEIYGLEV
                     IVVPTNVEMIREDCPDVIYRTVLEKFSAAADEIEESYKEGCPVLVGTTSIESSERLSK
                     LLKARKIPHQVLNAKHHEREAQIVAQAGHLGAVTVATNMAGRGTDILLGGNPEFLTRE
                     ALRRENGEAAESSEQYSALLEKYRGQCRAEREKVKALGGLKILGTERHESRRIDNQLR
                     GRAGRQGDPGSSRFYLSLEDDLLRLFGSERIQGLMEKLGMEEGESIEHNLLTKAIANA
                     QKKVEQLHFDIRKQLLAYDNVMNRQREAVYTERQQILRDEGIQEHVWEIIEGVIDDIL
                     ERYFPEEGEADPARAVARLKAIFGSGLEARLEGVDNVRDMLAHRDELVAEIRDRFIGK
                     VETLGKEEGRALLRFLVLHTLDSGWKDHLLAMDELRRGIGLRAIGQKDPLLEYQFESY
                     NLFQSMMERVRESVAELAFRVRVVSERGGASDGSNRREGREFILPFGASQEQQPPSLK
                     HGVEGVQQPVKKGPKTGRNDPCPCGSGKKFKHCCGKNA"
     gene            6440..8155
                     /locus_tag="PK849_01710"
     CDS             6440..8155
                     /locus_tag="PK849_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64869.1"
                     /translation="MIQECPSFSDYPGSSGVIWLKDVRYSLGADGAMTRDSVFVILAR
                     RGIDESWSRWTLPVPEDGGSVEVFSAALYDPGSGRMLAPVLPRKSQSGGVSVVELAFP
                     DLQEEYIISLSYREVFPKRFAVDDFIWVNETLPLWELQVTVDVPAGSDLAVSARGVGD
                     LRRENAGAVERHAWHMVNNLPWTTRTLRGDARSYLAFSTRKGLEPLARMLSAYENVLV
                     PQPSAPVEKLINQQNKLRTGSALISWMNAAPSFQGPFLSSIVRSTIPEEGPWSEWEKV
                     LILNRWMRKAGWESTVHWLTAHALNDGTPASGGSVLRPVLELNLVGISPFFLDLGQSQ
                     SPNETPPSLWGKHIYTSAGSSLTGRIVSGSSAAEHRLSVEWSLELSADGEANGRVDVF
                     VRNGWNAFFFPGGSPNAATLKRLVNELLPNLPFTEGTEAVKSIKYGWQATFPVSLRNS
                     IVSGGSMLFPYPAVLPSWLNELGRWSEEYRMNFPFVIEQNFTLKLPPKTDIVMLPASA
                     TRSLEKVKYEESVFHNRRRNTLTAGAKLVLSTDTINDAAGRSLAEAVQRWMSWGTKNL
                     PMRSR"
     gene            8183..>9779
                     /gene="argS"
                     /locus_tag="PK849_01715"
     CDS             8183..>9779
                     /gene="argS"
                     /locus_tag="PK849_01715"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPE64870.1"
                     /translation="MADVTKSLRGIVENALRKLADEKGIPSSDLPEIAFEKPKREGQG
                     DVSTNCAMKLCKLFGEKPLDLAGKIVERLEENEFVAKVEVAAPGFVNFFLSLKWMEKV
                     LSEILLEGKRYGSSSLGEDKKVQVEFVSANPTGPLHVGHGRGAAVGDIIASILSFTGW
                     RVEREYYINDAGLQMDILGRSTQSRYFEIACVPEKAPFPEDGYKGEYIYDIARAVREL
                     EGDRFLSMPLEESRPFFKKFAADAILETIKKDLADFGVKFDVWFSEKSLYERDLVPSA
                     MQTLKERGFAFENEGALWFKSTDFTDEKDRVLIRSNGVPTYFASDITYHKEKFDRGFE
                     KVIDIWGADHHGYVPRMKAGVEALGRNAEDLQVLLIQFVNLLRDGQQIPMSTRSGQFV
                     TLRDVLEEVGVDATRFFFVMRRSDSHLDFDLELAKQASNDNPVYYAQYAHARICSVLR
                     EAEARDVRLPGADEVNMEALSGDEERRLLARLGSFPEEMEKASRELAPHILVSYVLDL
                     AGDFHSFYNACRIHGEEEFVMKAR"
BASE COUNT         2211 a         2494 c         2820 g         2254 t
ORIGIN      
        1 gaaagccttt attgccgccg tcttcggagg cataggctcc atccagggcg cgatgatcgg
       61 cgggatgctg ctcgggctca tcgaaatcat ggctgtcgcc tttttcccgc acctgtcggg
      121 ctatcgtgac gcatttgcct tcgtgctgct catcgtcatc cttctcctga agcccacggg
      181 gctgatggga gagaagctgg aggacaaaat ctgatgaaca ccaggacacg caatttcata
      241 cttaactgcc tccttatcgc cgctctcggc gcatttctct actgggctca gaatactctc
      301 gacggttaca agattcaggt gctcaacctc atcgcggtca acgcaatcct tgccctcagt
      361 ctgaatctca tctacggttt taccggaatg ttctctcttg gccatgcggg gttcatggca
      421 ataggcgcat acgtatgctc aattcttatt ctttccccgg cccagaaaga ggctatgtgg
      481 attcttgagc ccttgatatg gcctctttca gtcatcaagg cgccgttttt cgtcgcggtg
      541 ctttcgggcg gtttgattgc ggctttcttc gcgctcctta tcgccatacc tgttctgcgc
      601 cttggcggag attaccttgg catcgccacg ctgggcttcg cggagatcat ccgtgtggtg
      661 ctcaccaacc tgagctttat taccaacggc gccctcggta ttaagggaat tcccccctat
      721 gcgaatatct ggtggaatta tgcctggctc cttctgactc tcttctttat catccgcctc
      781 gtgaacagta acttcggaaa tgtcctgaaa gcgatccgcg atgatgaaat cgcagcgaaa
      841 aacatgggaa tcaatacctt cttctacagg gtgctcgcgt tcacaatagg cgcttttttt
      901 gccggcgtag gcggcgctct catgggaagt ctcatcacca cgatagatcc gaggatgttc
      961 atgttcgtcc tcacgttcaa tatcctcatg atcgcggttg cgggaggttt ggggtcaatt
     1021 acgggcagca tcatcggaag tatcgtcatc accattcttc ttgaatggct tcgctttgtc
     1081 gagaacccct tagtgatcgg ctcctttgag attccgggta ttcctggaat gagaatggtg
     1141 atcttttccc tcgcgctcat tcttatcatc ctcttccgca gagaggggat tatgggaatg
     1201 cgtgagataa catgggatac gtttttctct cgcggaagac gaagccccga acatgaagaa
     1261 aagggaggaa aaatgaaatg acgactcccg catccatact tcggactgag aatctcacca
     1321 tgcgcttcgg aggcctcact gcggtgggga atttcagcat cgacgtgccg cagggaaaaa
     1381 tagtcggtct tatcggaccg aacggggcgg gcaagacgac cgttttcaat atgattacag
     1441 gcttttaccg tcctaccgaa ggacgtatct tctttgacgg acaggatata acggggtttc
     1501 agccgcatat ggtctgccgt tcggggatat cgaggacatt ccagaatatc cgcctcttca
     1561 caaacgagac ggttctggaa aacgtcatga tcggctgtca cgtccgcaga aagtcgaaat
     1621 ggtggatggc tccgctgggg cttccctcgt tcagggcgga agaggaggcc attcgcgaaa
     1681 aatcgatgga tctcttgaag tctgtgggct tggaggattt tgcggacgaa ccttcagctt
     1741 ccctctccta cggagcgcag cgccgccttg agattgcacg cgctcttgcg accgatccca
     1801 aatttctgct gctcgacgaa cccgcagccg gtatgaatcc gcaggaatcg caggagctca
     1861 tgtcgttcat ccggaatata cagaagcgct ttaacctgac catccttctt atcgaacacg
     1921 atatgaaagt tgtcatgggc gtctgcgagt atatctgggt tcttgactac ggcgtaaaaa
     1981 tcgccgaggg caaccccgac gctatccagt cggaccagaa ggtgatcgtg gcctaccttg
     2041 gagaggagta tgtaaagtat gttgagaatt gagaatctcc atgtgtatta cggcgccatt
     2101 cacgctgtga aagggatcaa ccttgaaatt cctgaaggcg ccatcgtcac tcttatcgga
     2161 gcgaacggcg cgggaaagag cagcaccatc cgcgctgtgg cgggacttgt aaaaaacgtc
     2221 aaggggaaga ttctctacac gccctccggc ggcgatgctt cggggcaggt aaacctcatc
     2281 ggcaaaactc cggaagaaat ggtgaagctt gggattgcca tgagtcccga agggcgtcgt
     2341 attctgccac accttactgt ggaggaaaat cttcagctcg gcgcctttat ccggaatgac
     2401 aaggacggaa tagaaagaga cagggatcat gtgtacgagc tcttcccccg gttgaaagag
     2461 cgctcttggc agaagggcgg gactctgtcg ggaggagagc agcagatgct cgccgtgggc
     2521 cgcgcgctta tgagccagcc gaagctcgtt atgctcgacg agccctcgct gggactcgcg
     2581 ccgctccttg tgaaagaggt cttcgaaata atcggcgaga taaacgctct gggaaaaaca
     2641 gtgctccttg tggagcagaa cgcctttgca gcccttaaga tcgcttccta cgcctatata
     2701 cttgaggtcg ggaatattgt attggaaggc aagggagaag agctcctcaa ggatgcccgt
     2761 gtaaaagagg cctatctggg gggataaatt gtttttttat gcagtgagaa gggtagccgg
     2821 gactttttcc ggttgccctt ttttgcttta tcaactacac tgtactcgca aatcatcttt
     2881 caggaggaac attttcgtgg gaaaaaaagt atggaatata cctaacacac tcagtctcct
     2941 tcgtgttttt ctcgcgcctc tcgttttgat tttcctgacg ttcaaattta cgtaccctgt
     3001 ctacagtgtc gaagatctcg ggattttggt gacggtcggc gatgttttgg ccggctccgt
     3061 gtttattctc gcagccctga cggatacggc cgacggctat atcgcccgga agaaaggaat
     3121 ggttacaaac cttggcaagt ttattgatcc gctcgcggac aagattcttg tcatggcggc
     3181 tcttatagct ttggtcgagc ttcaccgtat cccggcctgg atagtggttg tcattctcac
     3241 ccgggagatg atcgtcacgg gagtcaggat ggtcgccgca gctgaaggca ttgtcattgc
     3301 ggcctccaac gcgggcaaac tgaagacggt ctttcagatc gtggcaattg cgatgatcat
     3361 cttcaacatt cccttcggaa tccatgttat gtgggtggct atgctgatga cgctctggtc
     3421 ggggatggac tatctgataa aggggaaaaa actctttacc gagtagccgt gcgtttttca
     3481 gtcctgacgc gccgtttccg ggaggtccgc ataaatactt tgaccacgac gggttcggga
     3541 catataatga cccggtggta tgtgccgtgc gcgctgcacg gccgaatttt ttgttgaggt
     3601 gacgctatgc tcgaaatgct caaaaaggcc ctcggtctgg atcctaacga acgggccctg
     3661 aagcgctata caagtgtcgt agaacagatc aatgctcttg agcctgccct tttatcgctc
     3721 tccgacgaag agttgcgcga gaaaggcgct tcatttaaaa aacgcctcac ggagggtgaa
     3781 acccctgatg atatgctccc ggaggtcttc gcggtagtga gagaagtgtc caggcgcacc
     3841 attggactgc gtcatttcga cgtccagctg atgggtggta tggcgctgca cgagggaaag
     3901 atcaccgaga tgaagacggg agagggaaaa acgctcgttg caactcttgc cgttgtgtta
     3961 aacgctcttt caggggaagg agttcacctg gtgacggtga acgactacct cgccagaagg
     4021 gacgccgagt ggatggggcc gatctacaca ttcctcggac tctccgtcgg cgtcatttat
     4081 gcgtacatgg acccggaaga gcgtcttgcc tcctacagag cggatatcac atacggaaca
     4141 aacagcgaat tcggtttcga ctatctgcgc gacaacatgg cggtgaacga gtcgcatctt
     4201 gtgcagaggg gacacgcgta ctgtatcgtg gacgaggtcg actctatcct gatcgacgaa
     4261 gcgcgaacgc cgctcatcat ctccggtcca tcggacgaca acgtggagat gtacaccaca
     4321 tccgatcgga tagcccgtca gttgaaagag gggcgcgact tcgagaagga cgagaaagag
     4381 cggaacgttg cgatgaccga agccggtatc gccagatgcg aggagctcct caagctgccg
     4441 gacctctttt ccgatgccgc gcactccgat ctggcgcacc ggatcgtgca ggccctgaaa
     4501 gcgcgcattc tcttccagaa ggatgtccac tacgtcgtga aggacggaga aatcatcatc
     4561 gtcgacgaat tcacaggccg tttgatggtc ggccggcgtt actccgacgg actgcatcag
     4621 gccatcgaag ccaaggaggg tgtaaaagtc ggcagggaga gccagaccct ggccaccata
     4681 acgcttcaga actacttccg catgtaccgc aagctcgccg gaatgacggg taccgccgcg
     4741 accgaagctg aggagttcaa ggagatctac ggccttgaag ttattgttgt cccgacgaat
     4801 gtggagatga ttcgcgagga ctgcccggac gtcatctaca gaaccgtttt ggaaaagttt
     4861 tccgccgctg cggacgagat agaagaatca tataaggaag ggtgccctgt ccttgttggc
     4921 accacatcca tagagagctc ggagcgtctg agcaagctgc tcaaggcgcg taagatcccg
     4981 catcaggtgc tcaatgccaa gcatcacgag cgggaagcgc agattgtcgc ccaagcgggg
     5041 catctcggcg ctgtgactgt cgccaccaat atggcgggac gcggcacgga cattcttctt
     5101 ggcgggaatc ccgagttcct gactagggaa gcccttcgcc gggagaacgg agaagccgcg
     5161 gagagttcgg aacagtattc tgcgcttctt gagaaatacc gtgggcagtg ccgcgcggaa
     5221 cgcgaaaagg taaaggctct cggaggtctc aaaatactgg ggactgagcg tcatgaatcc
     5281 aggcggatcg ataatcagct tcgaggtcgc gcgggacgtc agggcgatcc tggctccagc
     5341 cgcttctatc tttcgcttga agacgatctt ctccgtcttt tcggctccga acgtattcag
     5401 gggctcatgg agaagctcgg aatggaggag ggagagtcta tagagcacaa tctcctcacc
     5461 aaagcgatcg caaatgctca gaagaaagtg gaacagctcc atttcgatat tcgaaaacag
     5521 ctcttggcgt atgacaacgt gatgaaccgc cagagagaag ccgtctatac cgaacgccag
     5581 cagatccttc gcgacgaggg gatccaggag catgtctggg aaattatcga aggtgtgatc
     5641 gacgatatcc ttgagcggta cttccccgag gaaggggaag cggatccggc gcgcgcggtt
     5701 gcccgtctga aggctatctt cggaagcggg cttgaggccc ggctggaagg tgttgataac
     5761 gtccgcgaca tgctcgcgca ccgtgatgaa ctggtggcgg aaatcagaga ccggtttatc
     5821 gggaaagtgg agaccctcgg taaagaggag gggcgggcgc tgcttcgttt ccttgtgctt
     5881 catacgctcg acagcggctg gaaggaccat ctccttgcaa tggacgaact ccggagggga
     5941 atcggccttc gagcgatcgg acagaaggac ccgttattgg aataccagtt tgaatcgtac
     6001 aacctctttc agtcgatgat ggagcgcgtg cgggaatctg ttgccgaact ggccttcagg
     6061 gttcgcgtag tgagcgaacg cggcggtgcg agcgacggaa gtaaccgccg cgaaggaaga
     6121 gagtttattc ttcctttcgg cgcctcgcag gaacagcagc cgccttcact gaaacacgga
     6181 gttgagggcg tacagcagcc tgtcaaaaag ggaccgaaga cagggaggaa cgacccctgt
     6241 ccatgcggca gcgggaaaaa attcaagcac tgctgcggta aaaatgcata acaagggcgt
     6301 ctggatgcgg aaaggagagt gtgcaatgaa cgcgtgcaaa aaaatgtttt caggtgtttt
     6361 gtctctactt gccgttggtg tagttctctt ttattcaccc ggagagagtt tttccgcgct
     6421 ttcggcgaac attccccgta tgatccagga atgtccgagt ttttccgact acccgggaag
     6481 ctccggcgtt atctggctga aagatgtccg gtattcttta ggcgccgacg gggctatgac
     6541 ccgcgattcg gtcttcgtta ttcttgcccg gcgagggatt gacgaatcgt ggagcagatg
     6601 gacgcttcct gtacctgagg acggagggag cgtggaggtc ttttctgcag ctctttacga
     6661 tcccggctcc gggcggatgc tcgctcccgt tcttcccaga aagagccagt ccggcggggt
     6721 ttcggttgtc gagctcgctt ttccggatct tcaggaagag tacattatct ctctttccta
     6781 ccgggaggtc tttccaaaac gctttgccgt agatgatttc atttgggtga atgaaaccct
     6841 cccgctctgg gagcttcagg ttaccgtcga cgttcctgcc ggcagcgatc ttgcagtctc
     6901 ggcgagaggc gtcggcgacc tgaggagaga aaatgccggc gctgtggaac gccatgcctg
     6961 gcatatggtg aacaatcttc cgtggacgac gcggacgctg cgaggagatg ctcggtccta
     7021 tctggctttt tccaccagaa agggacttga acctcttgcg agaatgcttt cagcctacga
     7081 gaatgtactc gttccgcagc cttcggctcc tgtggaaaaa ctgatcaatc agcagaacaa
     7141 gctgcgaacg gggagcgcgc ttatttcatg gatgaatgcc gcgccttcct tccagggacc
     7201 gtttctttcc tcgattgtac gttcgactat ccctgaggaa gggccctggt ccgaatggga
     7261 aaaggtgctt attctcaaca ggtggatgcg aaaagcaggc tgggaaagca ccgtacattg
     7321 gctgaccgcg catgcgctca atgacggtac tccagcttcg ggcggatcgg ttctccgtcc
     7381 cgtgctggag ttgaacctcg tcggcatctc cccgtttttc ctcgatctcg gacagagcca
     7441 gagtccgaac gaaacacctc cctcactgtg gggaaaacat atctatacct ccgccgggag
     7501 cagccttacc ggtcggatag tcagcggaag ttccgctgcg gaacacaggc tttcggtcga
     7561 atggagcctt gaactttccg ccgacggcga ggcgaatggg cgtgtggacg tattcgtccg
     7621 gaacggctgg aacgcctttt tcttccccgg agggagcccg aatgcggcga cgttgaaacg
     7681 tctcgtgaat gaactgttac caaatcttcc cttcaccgaa gggaccgaag cggtgaagtc
     7741 catcaagtac ggatggcagg cgacgttccc cgtttcgctg aggaacagta tcgtgagcgg
     7801 cggttccatg ctcttcccgt atccggcagt cttaccgtcg tggctgaatg aactgggccg
     7861 atggtcggaa gagtacagga tgaacttccc cttcgtgatc gagcagaact tcacgctgaa
     7921 acttcctccg aagaccgata ttgtaatgct gcctgcttcc gcgacacgct cgctggaaaa
     7981 agtcaagtat gaggagtcgg tctttcataa ccgccgccgc aacacgctga cggcgggggc
     8041 gaaacttgtg ctgagcactg ataccataaa cgatgcagcg ggaagaagtc tcgccgaagc
     8101 cgtccagcga tggatgtcct gggggacgaa gaaccttccc atgcgttcgc gctaaacgaa
     8161 gagaacctat ggaggtggct atatggcgga tgtaacaaaa tcccttaggg ggatcgttga
     8221 aaacgcgttg cgaaaactgg ctgacgagaa ggggatccct tcctcggacc tgccggagat
     8281 cgcatttgaa aaacctaaac gcgaggggca gggagacgtt tcgacgaact gtgcgatgaa
     8341 gctctgtaaa ctgttcggcg aaaaaccttt agatctggcc gggaagatcg tcgagcgtct
     8401 tgaggagaac gaatttgttg cgaaggttga agtagccgct cccggtttcg tcaacttttt
     8461 tctctcgctg aagtggatgg agaaggttct gtcagagatt ctcctcgagg ggaaaaggta
     8521 cggttcctct tctctcggcg aagacaagaa ggtgcaggtg gaattcgtca gcgcgaatcc
     8581 taccggaccg cttcacgtag ggcacggcag gggggcggcc gtcggcgata tcatagcgtc
     8641 cattctctcc ttcacggggt ggagggtaga gcgggagtac tatatcaacg atgcgggcct
     8701 tcagatggac atccttggac gctcgacaca gtcacgctat ttcgagatcg catgcgttcc
     8761 tgaaaaggcc cccttccctg aggatggcta taagggggag tatatctacg acatagcgcg
     8821 cgccgttcgc gagcttgagg gggatcgttt tctctccatg cctctggagg aaagccggcc
     8881 gttcttcaaa aaattcgcag ccgacgctat tctggaaacg ataaaaaagg acttggccga
     8941 tttcggtgtg aagttcgacg tttggttctc agagaagagt ttgtatgagc gtgatttagt
     9001 cccttcagct atgcaaacct tgaaagagcg cgggtttgct ttcgaaaatg aaggagcgct
     9061 ctggttcaag tcgacggact tcaccgacga gaaggacagg gtcctgatca gaagcaacgg
     9121 agtccccacg tacttcgcgt cggacataac gtatcacaag gagaagtttg acaggggctt
     9181 cgagaaagtc attgacatct ggggagcgga tcatcacgga tacgttccga ggatgaaggc
     9241 aggcgttgaa gctcttggaa gaaatgccga ggatcttcag gtgctgctca tacagtttgt
     9301 caatctcctt cgggatggac agcagatccc catgtccacg cgatcaggcc agttcgtcac
     9361 gctacgggac gtactggagg aagtcggagt ggatgctact cgctttttct ttgtcatgcg
     9421 gcgaagcgac tctcaccttg atttcgatct tgaactcgca aaacaggcat caaatgacaa
     9481 cccggtgtac tacgcccagt atgcgcatgc acgtatctgc agcgtcctcc gcgaagcgga
     9541 ggctcgtgac gtgcggcttc ccggtgcaga cgaggtaaat atggaggcgc tctccggaga
     9601 tgaagaaagg cgtcttcttg ccaggcttgg ctcctttcct gaagagatgg aaaaagcttc
     9661 gcgtgaactt gcgccgcata tattggtcag ctatgttttg gatctcgccg gtgatttcca
     9721 ctccttctac aacgcgtgcc gcatacacgg cgaggaggaa ttcgtgatga aagcgcgtc
//
LOCUS       DAOPKU010000030         9766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_7284_length_9766_cov_4.547420, whole genome shotgun sequence.
ACCESSION   DAOPKU010000030 DAOPKU010000000
VERSION     DAOPKU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7284_length_9766_cov_4.547420"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..2437
                     /locus_tag="PK849_01720"
     CDS             110..2437
                     /locus_tag="PK849_01720"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPE64871.1"
                     /translation="MNQAEREFLEELRRDFVPEAEEYLQTIVSGLLALEKSPASSSGV
                     IEEVFRAAHSLKGAAQAVQMPGVSSICQVVESVFSGMKKGVLHLSEGDFDILQRAADT
                     LSAMIGAPEKEDASGITPLIHEIESLLDPAGGAAPPKTPLLQGSRRPHAEEDEPEGKV
                     EALSLPDRALASHAVSPAVTEASPQPKEERSRHTGQETVRIAASKLDSLLLKAEELIS
                     VNLALEVRIENIREILSLSAEFKSEWEKTLDALRREEQAIEKLPQKLQGFLQSGRDSF
                     RKILETLRDMEKALRSDQRAASGLVRDLLEGARTVLMLPFSSLLQSFPKIVRDLAREL
                     GKEANVNIRGGEVEVDKRILEGLKDPLVHLVRNCVDHGLEKAEDRRAAGKPPEGTITL
                     TVSQTDGGRVEILVADDGKGINAVKLREIAVKNGLISREEAALLDDAAAMSLIFRSGV
                     STSPLITDISGRGLGMAIVQEGVEALGGSIVLESKPGKGSSFRLSLPLTLATFRGVLV
                     EEWGRVFVIPSYGVERVARVEKSKIRLIEQRESVSFNGTPLALARLGHILELPSPPER
                     LKIRETLPLVVIGAGGVSVAFGVDKVLNEQDILLKPLGKQLERVRNVSGATVLGSGMV
                     VPVLNTADLIRSVQRSGGGRGFLSDPEEKKTRRNVLIAEDSITSRTLLRNILSAAGFV
                     VQTAVDGQEAWDYLAKGGFHIVVSDVEMPRMNGFELTAKIRSDPATADIPVVLVTSLE
                     SREDRERGAEAGADAYIVKSGFDQGNLLDVLRRLL"
     gene            2464..3501
                     /gene="cheB"
                     /locus_tag="PK849_01725"
     CDS             2464..3501
                     /gene="cheB"
                     /locus_tag="PK849_01725"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HPE64872.1"
                     /translation="MADVLIVDDSALLRRILKQMLSKDPGLNVIGCVESGEAALQFLE
                     NTRVDVVTMDIHMPGMGGFDATRRIMVSPHPVPVVIISSCWDPNEVEKTFEAMDAGAV
                     AILPKPADISSGDDEYSAELLKTVNEAAVSKVGRLRQRKRPMKSDDAPRNPLQSSERR
                     ISLVGVGASTGGPQALAEFLARLPADFPRPLLVVQHIAAGFTSGLAEWLGKVTPLPVR
                     VAQNGQKAMNGVVHIAPDGFHMGVRQGGFLSLAKDPPEHMVRPSASVLFRSLAAAYGG
                     AAAVILFSGMGKDGAAEMKTLRNMGAATFAQDRESSVVWGMPGEAVRLDAAEHVGSPA
                     AIASLLLDQFR"
     gene            3594..5000
                     /locus_tag="PK849_01730"
     CDS             3594..5000
                     /locus_tag="PK849_01730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE64873.1"
                     /translation="MLSRIERADVMKEHAEILIVEDSLTQAKRLERLLVQNGYVVYVA
                     RNGAEGLEAAARKKPTVVISDVMMPEMDGYEMCRRIKNTEEIKGTPVVLLTSLSDPRD
                     VIKGLECGADNFLTKPYDGEHLIRRLEHILTNLELRKAGQAQMTVEVFFAGQYHRLTA
                     DRIQIIDLLLSTFEAAVLQSTQLEKLGGDYRNALEDVKRAQANFQTLMETTGDAVIVA
                     GDNGTIYYANPAAENLFDTKASDLVGRPFAFPLDVKGEEHREITISRSDGSKVVGDMR
                     VATSNWDGELVQFATIRDISEMVHLRELLKTEAITDALTGLHNRRGFMLLGEELLRKA
                     LEKESNVLCFFADLDGFKSVNDTLGHEEGDKVLQESAEALRKTFRSSDILARVGGDEF
                     AALVLHDAAGNETDIIGRLRQALDTINSRSLRSYRIVMSAGISLWEPENPISLGELMS
                     DADKKMYREKEERKKVSK"
     gene            5350..6663
                     /gene="lysA"
                     /locus_tag="PK849_01735"
     CDS             5350..6663
                     /gene="lysA"
                     /locus_tag="PK849_01735"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPE64874.1"
                     /translation="MTLGNSEHVNEKGHFLFGGWDTVELAGRFGTPLYVISEEMIRAR
                     CRSVRETFLNRWENVFAVYAGKAFLPLAMCRIVQSEGLGLDLVSGGELYTALAAGFPP
                     EKTFFHGNSKTREELRFALSSGVGRIVVDSMSELETLEEEAAAAGKKPAVLFRVAPGV
                     DAHTHKYILTGHTGSKFGFPLAGDSLNAAVRRASASEHVCLKGFHFHIGSQIFENTSH
                     VMAVEIMVRAIAGFKKEIGFVTEELNMGGGYGVEFAPDGRTPRIDSFTDAMMRALEEE
                     CRSAGVDRPRVIIEPGRWIVSEAGITLYTVQAIKKIDGIVTYASIDGGMTDNPRPSLY
                     GAKYWAVAAGKMNREASAKVTVAGRCCESGDILIENLKTPPLERGDLLAVLNTGAYTF
                     SMASNYNRLPRPAVVLASEGRADVIVPRQSYEDLIRGESLPEHLR"
     gene            6810..7967
                     /locus_tag="PK849_01740"
     CDS             6810..7967
                     /locus_tag="PK849_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE64875.1"
                     /translation="MKKSLALFGVLLLLVFAGSAFAADVIKIGHTVSLTGGASMWGQS
                     EARALDMLAKKINEAGGVLGKQIEFVRYDNRNDGVEAVNVARRLVSDGVVAVIGPAQS
                     GNSIASAPVFERGKIPMVVTTATNPYVTIDQRSNKVRPFAFRPCFIDPFQGTVAARFA
                     VTDLGAKKGAILYDVGSDYGQWLARYFEEAFVAKGGEIVAKEAFRTEELDYRAQLGKM
                     KDLAPDVIFIPTSQKEAAMAAKQARDLGINAVLLGTDNWGSPDLIELGGSAIDGGYFV
                     NLTDLADPDIQEFVAEYKAAFGEEPVLPNPVMAQDALLMIVDAIARAGSVEGDAVAEA
                     LATIKDLKVTSGVLTINAEDHNPLDKPAVIQQVDVTNKTFKFVKKYVSVDD"
     gene            8128..9000
                     /locus_tag="PK849_01745"
     CDS             8128..9000
                     /locus_tag="PK849_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE64876.1"
                     /translation="MFLQTLITGLSIGGIYALMAVGYSLVFSVLNFSNFAHGAVIMLG
                     AYVGLALATKFSLGLGLVIAGSVAGAGIIAVLNERLAYSSLRRRKAPSLYLMISAMGC
                     AVFLENMVYATIGSRFYAFPEFFGRQIIDLWGSSVSLLDMGAFLFALAAILGLHLFIS
                     RTRTGIAIRAGVSDMTMCSLMGVDLDRLIAIVFLLAGAFAGVAGVFLGIKYLVYPTMG
                     WVTNKAYIAAVIGGLGSLPGALAGGLILGVVETFVSTYVSSVLRDVFSFTLLIAFLVL
                     RPNGLLGRDGEEKV"
     gene            9054..9766
                     /locus_tag="PK849_01750"
                     /pseudo
     CDS             9054..9766
                     /locus_tag="PK849_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582982.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1988 a         2654 c         3083 g         2041 t
ORIGIN      
        1 atgaaggacc tggaagcggc ttcgggcggt ctgaaggaga tgggacaaaa actctcgctt
       61 ctgatcgaac ggtatacggt gtgaggcccc gaggtacgaa ggagcgctca tgaaccaggc
      121 ggagagggaa tttcttgagg aactgcgcag agattttgtt ccggaggcgg aggagtatct
      181 ccagaccatc gtctccggtc tgcttgctct tgaaaagagt cccgcaagct cttccggggt
      241 catcgaggag gtcttccggg ctgcccatag cctcaaaggg gcggcgcagg cggtgcagat
      301 gccgggggtc tcttcgatat gccaagttgt ggagagcgtc ttttcaggca tgaaaaaagg
      361 ggtccttcat ctctccgaag gagatttcga catcctccag cgtgctgccg atactctgtc
      421 cgctatgata ggcgctccgg agaaggagga cgcgagcggc ataactccgc tgattcatga
      481 gattgaaagc ctgttggatc cggcgggcgg cgccgctccg ccgaaaacgc ctttgttgca
      541 ggggagccgg agaccgcacg cggaggaaga tgagccggag ggaaaagttg aagcgctctc
      601 gcttccggat cgcgctcttg cgtctcatgc agtctctccg gctgtaacgg aagcctcccc
      661 gcagccgaaa gaagagcgct cacgacatac cgggcaggag accgtgcgta tcgccgcctc
      721 gaaactggat tcgttgctgc tgaaggccga agaactgatt tcggtgaatc ttgctctgga
      781 agtccgcata gagaatatac gggaaatact gtcgctttcc gcagagttca aaagcgagtg
      841 ggaaaagact cttgacgcgc tccggaggga agagcaggcc attgaaaagc ttccccagaa
      901 attgcagggt tttttacagt cgggaagaga ttcttttcgt aaaattctcg aaacgctgcg
      961 agacatggaa aaagcgctgc gttccgatca gcgggccgcg agcgggcttg tccgcgatct
     1021 cctcgaggga gccaggactg tgctgatgct tccgttttcg tcgctcttgc agagcttccc
     1081 gaaaattgtc cgcgatcttg cgcgcgagct tggtaaagaa gcgaacgtga acatacgggg
     1141 cggggaagtc gaagttgaca aacggattct tgaaggattg aaggatcctc tggttcatct
     1201 ggtgcgcaac tgcgtcgacc acggactgga aaaggcggaa gatcgccgcg ccgcaggaaa
     1261 gcctcccgag gggacgatca cgcttacggt ctctcagact gacgggggca gggtggaaat
     1321 tcttgttgcg gacgacggaa aagggatcaa tgcggtaaaa cttcgcgaga tcgccgtcaa
     1381 aaacggcctg atctcaaggg aggaggccgc gctcctcgac gatgccgctg caatgtcgct
     1441 catcttcagg tcaggcgttt ccacgagtcc gcttattaca gatatttcag ggcgagggtt
     1501 aggaatggcg attgttcaag aaggcgtcga agcccttgga ggtagcatcg tccttgaatc
     1561 aaagcccgga aaggggtctt cttttcgcct ttctcttccg ttaacgcttg caacgttccg
     1621 cggagtgctg gtggaggagt gggggcgtgt cttcgttatt ccatcctatg gtgtggagcg
     1681 cgtggccagg gtggagaaaa gcaaaatcag gcttatagaa caaagggaga gcgtgtcgtt
     1741 taacgggacg ccgctggcgc tggcgagact gggacatata cttgagctgc cgtctccccc
     1801 tgaacggctc aagatacggg aaacgttgcc tctcgttgtg attggagccg gaggggtctc
     1861 cgtggcgttt ggcgtcgaca aagtgctcaa tgaacaggat attctgctca aaccgcttgg
     1921 gaaacagctg gagcgggtgc ggaacgtctc gggcgccacc gtgctcggat cgggaatggt
     1981 cgttcccgtg ctcaacacgg ccgatcttat ccgttctgtt cagaggagcg gcgggggacg
     2041 aggttttctt tccgacccgg aagagaaaaa aacccgcaga aacgttctca tagccgagga
     2101 ttccattact tcgcgtactc tcctgcggaa tattctctca gccgcaggtt ttgttgtgca
     2161 gacagccgtg gatggacagg aggcgtggga ttaccttgcg aagggcgggt ttcatatcgt
     2221 ggtctccgac gtcgaaatgc cccggatgaa cgggttcgag ctgacggcga aaattcgctc
     2281 cgaccctgcg acggctgata tcccagtggt gctggtgaca tctcttgaat ccagagagga
     2341 cagggaacgg ggcgcggagg ccggagccga cgcatatatc gtgaaaagcg gtttcgacca
     2401 gggaaacctt cttgatgtat tgcgtcgtct tctgtagagt gtaaaagaga gggggcgttc
     2461 ggcatggcgg atgttctcat tgttgatgat tccgcgttgc tgcgcaggat cttgaagcag
     2521 atgctgagta aggatccggg gctgaacgtt atcgggtgtg tcgagagcgg cgaagcggcg
     2581 ttgcagttcc tcgaaaatac cagggtcgac gttgtgacta tggatattca catgccgggc
     2641 atgggcggtt tcgacgcaac acgccggatc atggtatctc cgcatcctgt gcctgtggtg
     2701 attatcagtt catgctggga tccgaacgaa gtcgaaaaga cgttcgaggc tatggatgca
     2761 ggcgccgtgg ctattctccc caagcccgcg gatatctcct caggcgatga cgaatactct
     2821 gcggagcttc tgaaaactgt aaacgaggct gccgtatcaa aagtaggacg tctgcggcag
     2881 agaaaaaggc cgatgaagag cgatgatgct ccccggaatc ctctccagtc ctctgaacga
     2941 cggatatcgc ttgtcggcgt cggcgcctcg acaggaggtc cgcaggctct tgcggagttt
     3001 cttgcgcggc ttccggcgga tttcccgcgc cctctcctcg tcgtgcagca tatagccgcc
     3061 gggtttacct cgggattggc ggagtggctt ggaaaagtga cgcctcttcc ggtacgtgtc
     3121 gcccaaaatg gacagaaggc gatgaacggc gtcgtccata ttgctcccga cggttttcat
     3181 atgggagttc gccagggagg ttttctttct cttgcaaaag atccgccgga gcatatggtt
     3241 cgtccgtccg cgtcagtgct cttccggagc ctcgccgcgg cgtatggggg agccgcggcc
     3301 gtcatcctct tctccggaat gggaaaggac ggcgccgcgg aaatgaaaac gcttcggaat
     3361 atgggggccg ccacgttcgc ccaggacaga gagagttccg ttgtctgggg catgcccggg
     3421 gaggctgtgc gcctggatgc agcggaacat gtaggatctc ccgcggcgat cgcctccctt
     3481 cttctggatc agttccgtta atctgagacc ggtggcgcgc atgattcttt ggcgagagtg
     3541 aaacgcactt cattcgggag gaaaatatac cgaaaatatg gagggaccac tccatgttga
     3601 gcaggattga aagggctgac gtgatgaaag aacatgctga gatcctcatt gtcgaggaca
     3661 gtctgaccca ggcgaagcgg ttggagcgtc tccttgtgca gaacggctac gttgtgtacg
     3721 tagccagaaa cggcgccgag gggttggaag ccgccgccag gaaaaagccc acggtggtga
     3781 tctcggacgt catgatgccc gagatggacg ggtacgagat gtgcaggcgc ataaagaaca
     3841 ccgaggaaat caaggggacg ccggtggtac tgctcacctc gctctccgat ccgagggacg
     3901 tgatcaaagg gcttgaatgc ggcgctgaca attttcttac caaaccgtac gacggcgagc
     3961 atctgatccg gcgtctcgaa catatactga ccaatttgga gctgagaaaa gccgggcagg
     4021 cgcagatgac ggtcgaggtt tttttcgcag ggcagtacca ccggctcacg gccgaccgga
     4081 tacagatcat cgacctcctg ctttccacat tcgaggcggc cgtgctgcag agcacgcagc
     4141 ttgaaaagct cggcggggac taccggaacg ccctcgaaga cgtgaaacgg gcccaggcca
     4201 actttcagac gctgatggaa actaccggcg acgccgtgat tgtggcgggc gataacggca
     4261 ctatctacta cgctaacccc gccgccgaga atctttttga caccaaggcg tccgacctgg
     4321 taggacgtcc ttttgccttc ccgctcgacg tcaaggggga agaacacagg gagatcacaa
     4381 tttcacggtc ggacgggtcg aaggtcgtgg gcgatatgcg cgtggcgacc tccaactggg
     4441 acggagagct cgttcaattt gctaccattc gtgatatttc cgagatggtc catctcaggg
     4501 agctcttgaa gaccgaggcg attaccgacg cgcttacggg gctccacaac cggagagggt
     4561 tcatgctcct cggagaagag cttctgcgga aggctcttga aaaggagtcg aacgtcctct
     4621 gcttcttcgc cgatctcgac gggttcaagt cggtgaacga cacgctcggt cacgaggagg
     4681 gggacaaagt cctccaggag tcggcggaag cgctcagaaa gaccttccgt tcttcggata
     4741 tactggcaag ggtcggcggg gatgaattcg cggctctcgt gcttcacgat gctgcgggaa
     4801 acgaaacgga tatcatcggg aggttgcggc aggcgcttga tacgatcaat tcgcggagcc
     4861 tgcgatccta ccgtatcgtg atgagcgcgg gaatatcgct ttgggagccg gaaaacccca
     4921 tttctctcgg cgagctgatg tcggatgcgg acaagaagat gtatcgtgaa aaagaggaga
     4981 gaaaaaaagt ttcaaagtag cagttttttt actgttgacg tttccgtatg tactggctat
     5041 aatgtcacga ataaataaga tgtacacgcg atgatgcaga ttgcctgcgt tcacggttgc
     5101 cgccagagag agagatccga aggctggaag gtctcttcgg caagggcgaa aggcgcaaag
     5161 actgcggagc gggggctgaa acggtcacga ccggaagtac gcccacggga ggctcccgtt
     5221 atagagccga gagcgggacg caaaaatgcg tccaagcagg gtggtaccgc gagacatatg
     5281 gctcgtccct tcattgggac gggccatttt tcatatttcg ctgcgaatga agaaagaggt
     5341 gcgcgtactt tgactttggg caacagtgaa cacgtcaatg aaaaggggca tttcctcttc
     5401 gggggttggg acacggtgga gcttgccggg cgattcggca cgccgctgta tgtcatctcg
     5461 gaggagatga tcagagcgcg gtgcagatct gtgagagaga cctttctgaa ccgctgggag
     5521 aacgtctttg ctgtgtatgc gggaaaggcg tttttgccgc ttgccatgtg caggattgtt
     5581 cagtcggagg ggttggggct tgatcttgtt tccggagggg aactgtacac tgctctcgcg
     5641 gcggggtttc ctccggaaaa gacttttttc cacggcaaca gcaagacaag ggaggagctg
     5701 cgtttcgctc tttcttcggg agtggggcga atagtcgtcg acagcatgag tgagctggaa
     5761 acgctcgagg aggaggcggc cgcagcgggg aaaaagcctg cggttctctt tcgggtggcg
     5821 cccggagtgg acgcgcacac gcataaatat atcctgacgg ggcataccgg ctcgaaattc
     5881 ggatttcctc tcgccggcga ctcgctgaac gcggccgtcc gccgtgcttc cgcttcggag
     5941 catgtgtgtc tgaaagggtt tcattttcat atcggctcgc agatcttcga gaatacgtcg
     6001 cacgtcatgg ccgtcgaaat catggtacgc gccatcgccg gattcaagaa ggaaatcggt
     6061 ttcgtcaccg aagaactcaa catgggcggg ggatacggcg tggaattcgc cccggacggc
     6121 aggactcccc ggattgattc ttttacggat gcgatgatgc gcgctctcga agaggagtgt
     6181 cgctcagcgg gcgtcgaccg tccgcgcgtg atcatagagc ccgggcgctg gattgtctcc
     6241 gaggcgggga ttacgctcta tacggtccag gcgatcaaaa agatcgacgg catcgtaacc
     6301 tatgcgagta tcgacggagg aatgaccgac aaccccagac cctcgctcta cggggcgaag
     6361 tactgggcgg tcgccgcggg aaagatgaat cgcgaggcgt cggcgaaagt gactgtggcg
     6421 ggacgatgct gcgagtcagg cgacattctt atcgaaaatt tgaaaacgcc gccgttggag
     6481 cggggggatc tgcttgccgt gctcaatacc ggcgcgtaca ccttctccat ggcgagcaac
     6541 tacaaccgac tcccgcgccc tgccgtggtg ctggcgagcg aggggagagc ggacgtgatc
     6601 gttccccggc agagctacga ggaccttatc cgcggagagt ctctgcccga acatttacga
     6661 tagaactggt tcctgaattt gtaggcggag gtggggcctg agaagaatcg ttgcgggaaa
     6721 aggcgggaat ccgtgaaacg ggcttcgcgc gggtgaacgg ccccggcgcg gggaatcacg
     6781 aaacacatca caaggaggta ggaagagata tgaaaaagag tcttgcgttg tttggagtac
     6841 tgcttctgct cgtctttgcc ggatcggcgt ttgcggctga tgtcatcaaa ataggccaca
     6901 cggtctctct caccggcggc gcttccatgt gggggcagtc cgaggcccgg gcgctcgata
     6961 tgctggcgaa gaagatcaac gaggccggag gcgttctcgg caagcagatc gagttcgtca
     7021 ggtacgacaa ccggaacgac ggcgtggaag cggtcaacgt ggcccggcgc cttgtctcgg
     7081 acggagttgt cgccgtcatc ggcccggcgc agagcggcaa ttccatcgcg tcggccccgg
     7141 tcttcgagag gggaaaaatt cccatggtcg tgacgactgc gaccaacccc tacgtcacga
     7201 ttgatcagcg ctcgaacaag gtccgtccct tcgccttccg tccctgcttc atcgacccct
     7261 tccagggaac cgtggcggcg cgtttcgccg tgaccgacct cggcgcgaaa aagggcgcca
     7321 tcctctatga cgtgggttcg gactacggcc agtggcttgc acgttatttc gaagaggcct
     7381 ttgtggccaa gggcggcgag atcgtcgcaa aggaggcctt ccgcaccgag gagcttgact
     7441 accgcgcgca gctcggcaag atgaaggacc tcgctcccga cgtcatcttc atcccgacaa
     7501 gccagaagga agccgctatg gcggcgaagc aggctcgcga tcttgggatc aacgccgttc
     7561 ttctgggtac cgacaactgg ggcagccccg atctcatcga gctgggcggc agcgccatcg
     7621 acggcggcta cttcgtcaac ctgacggatc tcgccgaccc ggatatccag gaattcgtcg
     7681 ccgagtacaa agccgcattc ggcgaggagc ccgtgcttcc gaacccggtc atggcgcagg
     7741 atgcgctgct tatgatcgtc gacgccatcg ccagagcggg aagcgtcgag ggcgacgcgg
     7801 ttgcggaagc cttggcaacc atcaaggatc tgaaagtcac cagcggcgtg ctcaccatca
     7861 acgcggagga ccataacccg ctcgacaagc cggcggtcat ccagcaggtg gacgtaacca
     7921 acaagacctt caagttcgtc aagaagtacg tttccgtcga cgattagcag cggcctgttt
     7981 tcacaggagc gtgctgaaca cgttctccga acatcgtatc gaaaccgaag ggtgcgggaa
     8041 tctctcccgc gcccttcatc cggaggcccg gggactccgc ggattcagcg ttcctcccca
     8101 atatgctcaa aggatgtgtg agccggtttg tttcttcaaa ctctgattac tggcctctcg
     8161 attggaggca tctatgccct gatggccgtg gggtactccc tcgtcttcag cgtgttgaat
     8221 ttcagcaact ttgcccacgg agcggtgatc atgctcgggg cgtacgtggg gctcgcactg
     8281 gcgacgaagt tcagcctggg gctgggactc gtgatcgcgg gaagcgtcgc cggagcgggg
     8341 attatcgcag tgctcaacga gcggcttgcc tactccagcc tccggcggag aaaggcgcct
     8401 tcgctctacc tgatgatcag cgcaatgggg tgcgcagtct ttttggagaa catggtgtac
     8461 gcgaccatcg gctcccgttt ctacgccttt cccgagtttt tcggacggca gatcatcgac
     8521 ctgtggggga gcagtgtcag tctcctcgac atgggggcct ttctcttcgc gcttgccgcc
     8581 attcttgggc tgcatctctt catcagccgg acgcggacgg gcatcgccat ccgcgcgggc
     8641 gtctcggaca tgacaatgtg ctccctgatg ggagtggacc ttgacaggct catcgccatc
     8701 gtctttcttc tcgcgggagc attcgcggga gtggcgggag tctttctcgg gataaagtat
     8761 cttgtctacc cgaccatggg gtgggtgacc aacaaagcgt acatcgccgc cgtgatcggc
     8821 ggtctcggca gcctccccgg agcgcttgcc ggagggctca tcctcggcgt ggtcgagacg
     8881 ttcgtctcga cgtacgtctc cagcgtgctg cgcgacgtct tcagcttcac gctgctgatc
     8941 gcgttcctgg tgttgcgtcc caacggtctc ctcgggcgcg acggagagga gaaggtctag
     9001 ccatggacta tattctctcc atcgttatcc ttgcggggat caatatgatc gccgtgctcg
     9061 ggctttccat cttcaccggg tttaccggcc tcttctcgct ggggcacgcc gccttcgtcg
     9121 ccgtcggcgc gtacacctcc gccatcctca cgtactacta ttacgtcgac tactatctgg
     9181 cgctcgctgc gggagtaccg cggcgtgctc ggcctcatca tccattccaa agggacgctc
     9241 ttctccctgg ccatcgccgc catcgtgggc gcggtcaccc tggtgaacag caccttctgg
     9301 caggtcgtga tcagccagcg gctcggggtg cccgacccgc tgctgccgtt cttcccgatg
     9361 gtccggtcga tcctgtcggt ggtgttcttc ttcacgatca tcccgcggct cacccacgcc
     9421 aagcacctga aggcacccac cctgatcggg ttcgcggtgt acctcgcggg gcagttgctg
     9481 cttgtcggca tccccgagcc ggacggggcc gcgaccgccg ccacctacgc catgctcgcc
     9541 ggctgtctgc tgctggacgc cttcggcgcg ggcatcctgt tcatgctcgc cgaatcgttg
     9601 gtcgcgctgc acgtcgacca gcacgagcgc tcccgggtga tggccatcca gcgcacctgc
     9661 gtgatgctgg tcaccgcccc ctttggctgg atctcgggct ggctctccag catggatcgc
     9721 acctggccct tcctgctcac cgcggtgctc ctggtgctcg gcctgc
//
LOCUS       DAOPKU010000031         9458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_7616_length_9458_cov_4.463363, whole genome shotgun sequence.
ACCESSION   DAOPKU010000031 DAOPKU010000000
VERSION     DAOPKU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9458
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_9458_cov_4.463363"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PK849_01755"
     CDS             complement(<1..816)
                     /locus_tag="PK849_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64877.1"
                     /translation="MPINISFLRKQFRYLVLVAALLLHGTLSSCAAPRVAGKILLLNS
                     YSIEDTWTAETNRGIAAAFEENGANVEFFVENLDITGTFSAMHLPELRELYRTRYTSM
                     RPDLIITTENDATSFALGQRGVLFPDVPIIFCSLTNKALLSIIPRTKGTGIFSENTSL
                     FILREIIRLHQNAPRIALVTDMSPRGFRDIAQMNEAATMLSIPRKRLQPLFGMDIEQL
                     TTSLSELPPNSVIILGHYNITREGDLVSFPRLIEAIQEASDLPVYSLIEEGAAL"
     gene            complement(841..2094)
                     /locus_tag="PK849_01760"
     CDS             complement(841..2094)
                     /locus_tag="PK849_01760"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPE64878.1"
                     /translation="MSAPQIVVIGAGPAGLIAAGRAAEKGARVILVEKNQTVGEKLLI
                     SGKRRCNLTNAEEDMDVFLSKYGPNGKFLYSSFAGFGPRETVSFFQSRGVPLKEERGK
                     RFFPATGGSEGVLAALLNYVREGGVTIYRKRESINLEIRDGRVRRFILRGQEVEGDAF
                     IICTGGKSFAKTGSTGDGYRFARRAGHTVIPPVPALCPVRTVEKWPLASRGMTLRNVS
                     LTLWKNDEVASQRFGELLLTHFGISGPIAMDMSKEIAAAMETGTARLTLDLKPALSLP
                     VLKNRIGRDFEKFSGRKFKDSLGELLPRGLIPAVLALKPLPEDKIVNSASNQEIETLA
                     ELLKAFPLTPDGLLGFDWAIVTSGGVALKEIEPRTMRSKLVENLFFAGEVLDLDGPTG
                     GFNLQMCWSTGYQAGESAADLSLPR"
     gene            complement(2091..4223)
                     /locus_tag="PK849_01765"
     CDS             complement(2091..4223)
                     /locus_tag="PK849_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPE64879.1"
                     /translation="MNTEICERVSKELKLPRDSVHAVLLLLEKDCTVPFIARYRKEAT
                     GSLDEEAVLAIKERSIRLEELEARRSAIHASLTDRGLLNPELEKAIANAASLTALEDI
                     YLPYRPKRKTRASIAAEKGLAPLADLLLRQEGNDPFSLAISFLSEEKGVATEEEALQG
                     AKDIIAERISENREARRQVRILFGRRGKLKSSCIKGKEEEGANFKDWFAWEEPAALAP
                     SHRILAMFRGEREKVLSLSLLPPEEEALRILRDIFVHGVGPDSILVNEAIIDGYRRLL
                     APSMETELRNALKKKADREAIAVFAKNVREILLASPLGPRNILAVDPGFRTGCKFVCL
                     NRQGDLLFNGTIFPHPPRADEAKSAEIMRKAIDEYGIEAVAIGNGTAGRETERFFRGL
                     GLPSDILITMVNESGASIYSASETARREFPEHDLTVRGAVSIGRRLLDPLAELVKLDP
                     KSIGVGQYQHDVDQKELKNALATVVESCVNAVGVELNTASLELLSFVSGIGSGTADRI
                     IKFREANGPFSSRKELLNVPRLGPKTFEQAAGFLRIRGGTNPLDASAVHPERYPLVER
                     IAADLGCSLPDLLSSPTLKNKIDVKQYISEDAGLPTLTDILNELEKPGRDPRETFEPF
                     TFQEGVEKIDDLLPGMPLPGIVTNITSFGIFVDVGVHQDGLVHRKRLPHGKEFRPGER
                     VNVSVLEVDRPRKRISLSMREEKKGTNR"
     gene            4341..4964
                     /locus_tag="PK849_01770"
     CDS             4341..4964
                     /locus_tag="PK849_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPE64880.1"
                     /translation="MGRNTWLPLYRRLVLGLSLPVDAEALRGKVALHFSDTPSTFYGV
                     MRRLIRFFEPQCIVHTGDLADDVKLEIRPGELPLYRRKLMELTSVLSSVQKSRIVLVT
                     GNHDHEESVREFFPDSSVIIKSGRIEMCGLDFNLSHEPEGLQTPYGHFNLFGHAPVPE
                     EAPSQGQVFLNGLYAIHVLRVDTGEVLALEYPGYVNDARLNKRRVGL"
     gene            4986..6008
                     /locus_tag="PK849_01775"
     CDS             4986..6008
                     /locus_tag="PK849_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64881.1"
                     /translation="MLSIVRGGPRVLFLKGERIALDYALSAAFSVTRHTFESALDCAG
                     EGQTIVALHGKEDDGGREFRIVSSSSDEVLAALVNDSSCRLESVRLLPRILFFRIFGD
                     KQRVLDQIENDLAGGGIPGATVRRGRLRALLGSSRRNEIAVCFTYAPLNGLIAVADML
                     DDVLFVEETSFYDLNVFLRSRGLWYFSEGLENRSWNEMEIRIYDSWGFFMEHAERLRA
                     LLGSLEIGMILGEGWGKDFAHILMPVQIYRLRLFTFLSTLLVKELLMGLEYAENGDRL
                     ADFDLYSGRDKISWGSLKDRGAGRANLGKTAREKLFSRLSVRDRERLTAMEHRLLRLR
                     QGGNNG"
     gene            6293..6553
                     /locus_tag="PK849_01780"
     CDS             6293..6553
                     /locus_tag="PK849_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HPE64882.1"
                     /translation="MEVLKVSAKSQPKSVAGAIAAVLREKGAVEVQAVGAGAVNQSVK
                     SIAIARGYVAPNGIDLVCIPAFSKISIDDEERTAIKFQLESR"
     gene            6766..8046
                     /gene="hisS"
                     /locus_tag="PK849_01785"
     CDS             6766..8046
                     /gene="hisS"
                     /locus_tag="PK849_01785"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPE64883.1"
                     /translation="MAEMSAPKGVRDILPEESWKWAYVFRMVQETARHFGYSEVHLPI
                     FEHTELFSRGIGDTTDVVEKEMYTFLDKGDRSITLRPEATASMVRAYLEHRMGTMAQP
                     VKMWCAGPMFRYERPQKGRYRQFWQVDFESIGASSPYADVEIIAISLELFRRLGLRHL
                     EVVINSVGCPECRPDYRKRLTDYFESRKDNLCETCLGRLERNPLRILDCKRDGCKAVT
                     EEAPDVYDSLCGECREHFDVVKDSLDALGASYRLDKRLVRGLDYYTKTAYEILSGELG
                     SQNAVCGGGRYDNLAEAIGGPHVPGVGFAAGVDRIVMVMEQQECSFGSVPTVEVFAVA
                     LDDISRRNVLQLVDMLRRNGIVAEMDLAGKSMKAQMKAASSRGARFACILGESERTRG
                     TVMVKDLATAEQQEITLSLLVAELKNKLTSTRKG"
     gene            8070..>9458
                     /gene="aspS"
                     /locus_tag="PK849_01790"
     CDS             8070..>9458
                     /gene="aspS"
                     /locus_tag="PK849_01790"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPE64884.1"
                     /translation="MEGAQRYFDKEWQRTVYCGDVSGKSGEELPVVVNGWVRRRRDLG
                     GLIFIELWDHTGAVQVVINPELTPEVHERARELRSEFVISVKGKIRVRPEGTANPSMK
                     TGEWEIVAGDLLRLAPAAPLPFEIGELTDKVDENLRLHNRYLDLRREKMQRNLRLRHK
                     IAMYTRNFLSDGGFLEIETPMLTLSTPEGARDYLVPSRVNPGSFYALPQSPQIFKQIL
                     MISGFDRYFQIVKCFRDEDLRADRQPEFTQIDIEMSYLVEEDIFSLIEKYLQGLFKET
                     IGVDVATPFRRMTYWEAMDRFGSDKPDLRIPFEIVDLSPVFAGSSFEPFRDIAAQGGY
                     VRGIPLPGGASLSRKQLSDIEEKAKRLGARGLSPFQIKDGELKGPLVKYLDESSRSLL
                     KSSSSIDDGDALFVIAGSDRREISEILGALRLELAREHGLVAKGAWEFLWVVDFPLFE
                     RDEEAGRWSAVHH"
BASE COUNT         2056 a         2439 c         2727 g         2236 t
ORIGIN      
        1 taatgcggcc ccttcttcta tgagagagta cacagggaga tccgaagctt cttgaatagc
       61 ctctatcaga cgggggaaag aaacgagatc tccctcccgg gtaatattgt agtgcccgag
      121 gatgataaca gaattcggag gaagctcgga aagagatgtt gtgagctgtt cgatatccat
      181 tccgaaaagc ggttgcagac gttttctcgg aatcgagagc attgttgccg cctcgttcat
      241 ttgtgcaata tcacgaaacc cgcgaggcga catatccgta acaagcgcga tacggggggc
      301 gttctgatga aggcggatta tttcccgcaa tataaaaagc gaggtgtttt cactgaagat
      361 tcccgttccc tttgtccggg ggataataga gagaagcgct ttgttcgtaa gggagcagaa
      421 gatgatggga acgtcgggga aaaggacgcc tctttgtccg agagcgaatg atgtagcgtc
      481 attttccgtt gtgatgataa gatccgggcg cattgaagtg taccgagtac gatagagctc
      541 ccggagttcc gggaggtgca ttgcggagaa cgtccccgtg atatcgaggt tttcaacaaa
      601 gaattcaaca ttggctccgt tctcctcaaa agctgctgcg atgcctcgat ttgtctcggc
      661 agtccacgtg tcttcaatgg agtatgagtt gagcagtaga atctttccgg ctaccctggg
      721 agccgcgcac gatgaaagtg tgccgtggag aagaagcgct gcaacgagta cgagataccg
      781 gaattgcttt cgtaggaaag aaatgttgat cggcactatg tatctcctct ctcgctttgt
      841 ttagcgcggc aatgagaggt cagccgcgct ctctcctgcc tggtaccctg tgctccagca
      901 catttggagg ttgaacccgc ctgtaggacc gtcgagatcg agcacttctc cagcgaaaaa
      961 gaggttctcc accagcttgg atcgcatcgt tcggggctcg atctccttca acgcgacacc
     1021 gccgctggtc actattgccc agtcgaatcc gagaagtccg tccggagtca gaggaaaggc
     1081 tttgagcagc tcggcaagcg tttcgatttc ctgattgctt gcggagttca caattttatc
     1141 ctccggtaga ggcttcagag cgagtactgc aggaatgagc ccccttggga gaagctcccc
     1201 gagagagtcc ttgaattttc tgccggagaa cttctcaaaa tccctgccaa tcctgttttt
     1261 cagcacagga agagaaagcg ccggcttgag gtccagagta agcctggccg tgcccgtttc
     1321 catggcggct gcaatctcct tgctcatgtc catagcaatc ggaccgctga tgccgaaatg
     1381 tgtcagcaga agctctccaa agcgttgcga tgccacttca tcatttttcc aaagagtgag
     1441 tgagacgtta cgtagtgtca tcccccggga agcaaggggc cacttttcca ctgttctgac
     1501 cgggcagagc gccggaacag gaggaatgac ggtgtgtccc gccctgcgtg cgaagcggta
     1561 accgtcgcct gtcgatccag tcttcgcaaa ggactttccc cccgtacaga tgatgaacgc
     1621 gtctccctcc acttcttgtc ccctgagtat gaagcgacgg acccgaccgt cgcgaatctc
     1681 gaggttgatc gattcgcgct tgcggtagat ggtgactccg ccttcccgta catagttcag
     1741 aagggctgcg agcaccccct ccgatccgcc tgttgccggg aagaaccgtt tgccccgttc
     1801 ttccttgagc ggcactcccc tggattggaa aaaagaaact gtctcgcggg gtccgaagcc
     1861 ggcgaaagag gagtagagaa attttccgtt cggtccgtac tttgagagaa agacatccat
     1921 gtcttcctcg gcgtttgtca ggttgcaccg tcttttcccg ctgataagga gcttttcacc
     1981 gacggtctga tttttctcga ccagaatgac cctggcgcct ttctcagccg cgcgtcccgc
     2041 ggcaatcagt ccggcgggac cggcgccgat taccacgatc tggggagcgc tcatcggttc
     2101 gtaccctttt tctcttcacg catggagagc gagatacgtt tgcggggacg atccacctca
     2161 agtaccgaga cgttgacgcg ctctccggga cgaaactctt tcccgtgcgg caggcgcttc
     2221 ctgtggacga gtccgtcctg gtgtacgccg acgtcgacaa agataccgaa agaagtgatg
     2281 tttgtaacga tcccgggaag cggcatgccg gggagcaggt cgtcaatctt ctcgacgccc
     2341 tcttgaaatg tgaacggctc gaatgtctcc cgcgggtcgc gcccgggttt ttccagttcg
     2401 ttcagtatgt cggtaagcgt gggaaggccc gcgtcttcgg agatgtactg ctttacatca
     2461 attttgttct tcaacgtcgg gcttgagagc aggtccggga gagaacaccc cagatccgcg
     2521 gctatacgct cgacaagcgg atagcgttcg gggtggactg cgcttgcgtc gagaggattc
     2581 gttcctccgc ggattcgaag gaaccctgct gcctgttcaa atgttttcgg tccgagccgc
     2641 gggacgttca ataactcttt tctggaggag aacgggccgt tcgcctccct gaatttaatg
     2701 atacggtccg ctgttccgga gcctatgccg gagacgaaag agagcagttc aaggctcgcc
     2761 gtgttcagtt ctacgccgac tgcgttcacg caggattcga caaccgtcgc gagcgcattt
     2821 ttcagttctt tctgatcaac gtcgtgttgg tactgaccga cgcctatcga tttaggatca
     2881 agtttgacaa gttcggcgag cgggtcgagc agtctgcgcc cgatggacac tgcgccgcgc
     2941 accgtgagat catgttccgg aaactctcgc cgcgccgtct ccgaagcgga atagatcgac
     3001 gcgccgctct cgttcaccat cgtgatcaga atgtccgaag ggagtccgag tccgcggaaa
     3061 aaacgttctg tttcccgccc cgccgttccg ttgccgatgg cgacggcttc aattccatac
     3121 tcgtctattg ccttccgcat gatctccgcg ctttttgctt catcggcacg gggaggatga
     3181 ggaaaaatag tcccgttaaa gagcagatca ccctgccggt taaggcagac gaacttgcag
     3241 cctgtccgga aacccggatc aactgcgagt atattccggg ggccgagcgg cgaggcgaga
     3301 agaatctccc ggacgttctt tgcaaagaca gcgatagcct ccctgtccgc cttttttttc
     3361 agcgcgttgc ggagttcggt ctccatggag ggcgcgagca ggcgccgata gccgtcgata
     3421 atcgcttcat tcacaagtat ggaatcaggg cctacgccat gcacaaaaat atcccggaga
     3481 atacgaagcg cttcttcctc gggtgggagc agagagagcg aaagcacctt ctcccgttct
     3541 cccctgaaca ttgccagtat cctatgggaa ggagcaagcg ccgcgggctc ttcccaagcg
     3601 aaccagtcct tgaaattggc tccttcttct tcttttccct tgatacagct gcttttcagc
     3661 tttcctctcc tgccgaagag aattcggacc tggcggcgcg cctccctatt ttcgctgata
     3721 cgttcggcga taatatcctt tgcgccctgc agagcctctt cttctgttgc aactcctttt
     3781 tcttcggaga ggaaagagat tgcaaggctg aaaggatcgt tcccctcctg acggagtagc
     3841 aggtcggcga gtggagcaag tcctttctca gccgctatgg acgctctcgt cttgcgtttc
     3901 ggcctgtatg ggagatagat atcttcgagc gcagtaagag aagctgcgtt cgcgattgcc
     3961 ttttccaatt caggattaag aagcccacgg tcagtaagcg aggcgtgaat cgcgcttctg
     4021 cgcgcctcca gctcttcaag acggatcgaa cgctccttga tagcaaggac ggcctcttca
     4081 tccaatgagc ctgtcgcctc tttcctgtac ctggcaatga acggcacagt acagtctttt
     4141 tcgaggagca ggagcactgc gtgcacggag tcgcgcggca gtttgagttc ttttgacact
     4201 cgttcgcaaa tttcggtgtt catcaggaat cctccgatcc cgcaaatgca tgtatgaggc
     4261 ctcttgtcct ctcattctag cacgagaaga aatgcgggga aatatcgtgg tagaatctgt
     4321 tgtgaagaga gggttgcttt gtgggaagaa atacgtggct tccgctgtac agaagacttg
     4381 ttttggggct gtctctcccc gttgacgcag aagcgcttcg gggaaaagta gcgctgcact
     4441 tctcggatac tccgtccacc ttttacggag tgatgaggcg tttgatacgg tttttcgagc
     4501 ctcagtgcat cgtccataca ggagacttgg cggacgatgt gaagcttgag attcgtccgg
     4561 gcgaactccc tctgtatcga agaaaactga tggagctcac ttcggtgctc tcatctgttc
     4621 agaagagcag gatagttctt gttacgggga atcacgatca cgaagagagt gtccgcgaat
     4681 tcttccccga cagttctgta attattaaga gcgggcgaat cgagatgtgc ggtctggatt
     4741 tcaatctaag ccacgaaccg gaaggattgc agactccata cggccatttc aaccttttcg
     4801 ggcatgcgcc tgttccggag gaagcgccga gtcaaggaca ggtgttcctt aatggtcttt
     4861 atgcgattca tgtcttacgt gtcgacacag gggaagtatt ggccctggag taccccgggt
     4921 atgtgaacga cgcccggctc aataaaagaa gagtcggcct ttgattacag tagggaggtg
     4981 tgttgatgct ttcgattgtt cgaggcggtc ctcgggttct cttccttaag ggagagcgaa
     5041 tcgctttgga ttatgcgctt tccgccgctt tctccgtaac gaggcatacc tttgaaagcg
     5101 ccctggactg cgccggagag gggcagacga ttgtcgcgct tcacggaaag gaggatgacg
     5161 gagggagaga gttccggatt gtttcctctt cctctgacga ggtgcttgcc gcccttgtga
     5221 acgacagctc ctgccgcctt gaatcagtcc gtctgcttcc tcgtattctc tttttccgta
     5281 tcttcggcga caagcagcgt gtgcttgatc agattgaaaa cgatctcgca gggggaggta
     5341 ttccgggagc aaccgtacgg agagggaggc tgcgagctct tttagggagt tcacgaagaa
     5401 acgagattgc ggtttgcttt acgtacgctc ctctgaacgg tctcatagcc gtggcggata
     5461 tgctggacga cgtgcttttt gtggaagaga cttcctttta tgacttaaat gtgtttctcc
     5521 ggagcagggg cctctggtat ttcagcgaag ggctggaaaa ccgctcatgg aacgagatgg
     5581 aaatacggat atacgattca tggggttttt tcatggagca cgccgagcgt cttcgcgcac
     5641 tcctcggctc tctggagata ggcatgatcc tcggagaagg ctgggggaag gattttgcgc
     5701 atattctgat gcctgtccag atataccgcc ttcgtctttt tacttttctt tcgacgctgc
     5761 tcgtcaagga gttgctcatg gggctggagt acgcggagaa cggagacagg ctggcggact
     5821 ttgatctgta tagcggacgg gataaaatct catggggaag tttaaaagac cgtggagcag
     5881 gacgcgcgaa cctgggaaaa acagcacgcg aaaagctttt ttcccggctt tcagtgcgtg
     5941 accgcgaacg gttgaccgct atggagcacc gtctgcttcg tctgcggcag ggtggaaaca
     6001 acgggtgaaa atgagccgtc gcaagacagg agtccatctc cttgcgcctc gaattactga
     6061 aacaggatga tagagcgttt ttttgaaaaa agagaaaaaa gcgattcact ccttgcaaag
     6121 aacttgctcc tgcgattata ctaatttaga aactttcgga gaactctagt ggcagactct
     6181 taggctttct cgccccggcg aattgaagga gaacttgttt cttcgagctc atgattcgcc
     6241 ggatgcgttc cgatatgcgc agatcgtcaa tctacgaagg aggtcgctac ttatggaagt
     6301 gctcaaggtc tctgcaaagt cacagccaaa gtcggtcgcg ggagccattg cagcggtact
     6361 gcgggagaaa ggggcggtcg aagttcaggc agtgggcgcc ggagccgtga accagtcggt
     6421 gaagtccatt gcaatcgcaa gggggtatgt cgctcccaac ggtattgatc ttgtgtgtat
     6481 accggccttt tcgaaaattt ccattgacga tgaagaaagg acggccatca agtttcagct
     6541 ggaatcgcgc tgaattcacg cggcgtattc aacgcatttg agcccggccc cgaattgagg
     6601 gtcgggtttt gtttttttta gatcatctct ggaaaaaaaa cgaaggaatg ggtatactcg
     6661 cttgcataat gtattcgccc tcgaggtgtc tccatctttt ctttgaggtg aaggaatgca
     6721 acagcgtttc ggcggggcaa aaaccgccgg tcgggaggag aaaacatggc ggaaatgagc
     6781 gcaccaaaag gagttcggga tattcttccg gaggaatcgt ggaaatgggc gtacgtcttt
     6841 aggatggtgc aggagacggc ccgtcatttt ggatattcgg aagtgcatct tcctattttt
     6901 gaacatacgg agctcttctc aaggggaatc ggcgatacaa ccgacgttgt cgaaaaggag
     6961 atgtacactt ttctcgataa aggggacagg agcattacgc tccgtccgga ggctacggcg
     7021 tccatggtgc gcgcatatct tgaacaccgg atgggaacga tggctcagcc ggtgaaaatg
     7081 tggtgcgccg gccccatgtt ccggtacgaa cgccctcaaa agggacggta cagacagttt
     7141 tggcaggtcg atttcgagag tatcggcgct tcttctccct atgccgatgt cgagattatc
     7201 gccatctccc tggaactctt ccggcggctg gggcttcgtc accttgaggt ggtgatcaat
     7261 tccgtcgggt gtcccgagtg ccgccccgac tataggaagc gtctcaccga ctacttcgag
     7321 tcgagaaagg acaatctctg cgagacgtgt ctcgggcgcc tcgaaaggaa ccccctgcgc
     7381 atattggact gcaagcggga cgggtgcaaa gcggtcactg aagaggctcc cgatgtctac
     7441 gacagcctct gcggcgaatg ccgcgaacac ttcgacgtgg tgaaagacag ccttgatgct
     7501 ctcggcgcct cctaccgtct tgataagcgc cttgtccgcg gactggacta ctatacgaag
     7561 acggcgtacg aaattctctc aggagagctc ggctcccaga acgccgtttg cggcggaggc
     7621 cgttacgaca acctcgcgga agcgatcgga gggccgcatg tccccggcgt cggatttgcc
     7681 gcaggcgttg atcgtattgt gatggtgatg gagcagcagg agtgttcttt tggttccgtt
     7741 cccaccgtgg aagtcttcgc ggttgccctg gatgatatct cgaggcggaa tgtcctgcag
     7801 cttgtggata tgcttcgccg gaacggaatc gtagcggaga tggatcttgc cgggaagagt
     7861 atgaaagcgc agatgaaggc cgcttcatcg cgcggcgcac gattcgcctg cattctcggc
     7921 gagagcgaac gcacgcgcgg cacggtaatg gtgaaggatc tcgcaacggc ggaacagcag
     7981 gaaattacac tgtccctgct cgttgccgaa ctgaaaaata aactgacctc tacccgcaaa
     8041 gggtaaacgg ctcctggagg gaacgcttca tggaaggtgc gcaacggtat ttcgacaaag
     8101 agtggcaaag aacggtctat tgcggtgatg tctccggaaa gagcggagaa gagcttcctg
     8161 tcgtcgttaa cgggtgggtc aggcgaagga gagatctcgg cggtctcatc ttcattgaac
     8221 tttgggatca tacaggcgca gttcaggtgg taatcaaccc cgagctcacc cctgaggtac
     8281 atgagcgagc ccgggaactg cgcagcgaat tcgttatttc ggtgaagggg aagattcgcg
     8341 tccgtcctga agggaccgcg aatccctcga tgaagacagg agagtgggag atagtcgccg
     8401 gagatttact ccgcctcgcc cccgccgctc cgttaccttt cgagataggc gagttgaccg
     8461 acaaagtgga tgagaacctg cgtcttcaca acaggtatct cgaccttcgc agagagaaga
     8521 tgcagcggaa cctgcgtctt cggcataaga tagcaatgta cacgcggaat tttctgtccg
     8581 acggcggatt tctcgagatc gaaactccca tgcttaccct ctcgactccg gagggagccc
     8641 gggattatct tgtgccgagc cgcgtcaacc cgggttcgtt ttacgcgctt ccgcagtcgc
     8701 cgcagatctt caagcaaatc ttgatgatca gcggtttcga caggtatttc cagatagtga
     8761 aatgcttccg tgacgaggat ctccgggcgg accggcagcc tgagttcaca caaatcgaca
     8821 ttgaaatgag ctacctcgtg gaggaggaca tcttctctct catcgagaag taccttcagg
     8881 ggctgttcaa agagacgatc ggcgtggacg tcgcgacgcc gttccgccga atgacctact
     8941 gggaggccat ggaccgtttc gggagcgata agccggatct ccgcatcccg tttgaaattg
     9001 tcgatctctc cccggtattt gcagggagct cctttgaacc gttccgcgat atcgcggcgc
     9061 aaggagggta tgtccgcggt attcctcttc ccggaggggc ctcgctctca agaaagcaac
     9121 tttccgatat cgaggaaaaa gcgaagcgcc tcggcgcccg cggtctttct cccttccaga
     9181 tcaaagacgg agagctgaag ggacctttgg taaaatacct tgacgagagc tcccgatcgt
     9241 tgctcaaatc atcctcgtcc attgacgacg gcgacgcgct ctttgtgatc gccgggagcg
     9301 accgtcgcga gatttccgaa attctcgggg cgcttcgcct tgaactggct cgtgagcatg
     9361 ggcttgtcgc gaaaggcgcc tgggagtttc tctgggttgt ggattttccc ctgttcgaac
     9421 gcgatgaaga ggcgggacgc tggagcgcgg tgcatcac
//
LOCUS       DAOPKU010000032         9438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_7639_length_9438_cov_5.126825, whole genome shotgun sequence.
ACCESSION   DAOPKU010000032 DAOPKU010000000
VERSION     DAOPKU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7639_length_9438_cov_5.126825"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PK849_01795"
     CDS             complement(<1..991)
                     /locus_tag="PK849_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE64885.1"
                     /translation="MGSIRSRQSNPPFRMRVYGLLLFVFLILCAACFPTDLFAAKIVR
                     VGFYENSPKIFTAESGLPAGIFADIIRYIAEKEGWELRFVHGTWSQGLDRLEKGDIDL
                     MPDVAYTEARGKLFSFHSDPALYSWFQVFSRRGSGIRSIPDLDGKRVAVLYRSIQQEI
                     FDRIIRDFGLSVSFVTFSDFESAFQAVANGKTDAVLTNRFFGAHHMRAYNLEDTAISF
                     NPTRLFFAAPRDSDPAIPAAIDKHLREMKRNPDSVYFSSLAQWTSSPVFMVIPTWLKS
                     TATLLAFLTLLGGILSFAFKRQADSRAAELTRLFDNAPMGLFFSGRDGTIKRTN"
     gene            complement(1174..1914)
                     /locus_tag="PK849_01800"
     CDS             complement(1174..1914)
                     /locus_tag="PK849_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE64886.1"
                     /translation="MKVFLVMAFCLFSVSAWALTVDDVTFMTEDYPPFNMAGADGTAT
                     GASVEVLVGIFEKLGAAKTVKDIQVLPWARGYREVQNTPNTCLFLMTFTDERKPLFKW
                     VGPLAPTKVGVLALKSKGLKITSASDLANLKAGVVRDDIGDELAKQAGIQQIERVPSN
                     DQNIKKLTEGRIDIWVFEESVAKWQIQSLGFNADDYESVWTLSESDLYYAFHKDTDDA
                     LIASIQKTLDDMKADGSYEAVMKKYNMR"
     gene            complement(2144..2893)
                     /locus_tag="PK849_01805"
     CDS             complement(2144..2893)
                     /locus_tag="PK849_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE64887.1"
                     /translation="MKLLKIALVLVFCLCSGSAWALTVDDITYMTEDYPPFNMAGADG
                     VATGAAVDTLTTIFERMGAAKTAKDIQVLPWARGYREVQSTPNTCLFLMTLTDERKPL
                     FKWVGPVVVNTFDAIALKSKGMKIASAEELVNLKAGVIRDDVGDALAQKAGIKQIERV
                     PSNDQNIKKLNEGRIDIWVFGESSAKLQVKEMGLNPDDYESVWRMSESGLYFAFHKDV
                     DDALIESMQKVLDEMKADGAYEAIMKKYNVQ"
     gene            3416..4300
                     /gene="dapA"
                     /locus_tag="PK849_01810"
     CDS             3416..4300
                     /gene="dapA"
                     /locus_tag="PK849_01810"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPE64888.1"
                     /translation="MFGGTGTAVVTPFKDGSVDYESFERFLNWQIEGGVEFLVVLGTT
                     GESPTITADERAEIIKFAVKAAAGRVRVVIGTGTNSTASTILLSQQAEGLGADGLLVV
                     GPYYNKPTQEGFYQHFKAVAESVKTPIIVYNVPGRTGSNIAPETVYRLSKLRTIAGIK
                     EAAGDMAQIDMLVRLIKKERPDFAILSGNDDQAFHLVNSGGDGVISVLSNVMPRETSD
                     MIRLALAGEVEKARALHHRMFPLMKNLFVEANPIPVKYAASRLGFCRNELRLPLVPAS
                     EKCVAIVDASMKECGTAL"
     gene            4297..4947
                     /locus_tag="PK849_01815"
     CDS             4297..4947
                     /locus_tag="PK849_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPE64889.1"
                     /translation="MRYGLVGASGRMGGEIRKAFAAHELTFSLDVGGETHDAAPQVLV
                     DFSLPAAFPSTFAVARRFVCPLVIGTTGLSAEQLAELRTLGEQVAVVQSFNFATGVTL
                     LKMMLREFAPLFADWDVEISETHHNKKKDAPSGTAILLREATGRDCPTHSLRLGGIPG
                     DHTVCYANEGEVLTLTHRALSRSVFALGALKAAEFAVSAAPGFYSFEEVLSCGLKR"
     gene            4929..5630
                     /gene="dapD"
                     /locus_tag="PK849_01820"
     CDS             4929..5630
                     /gene="dapD"
                     /locus_tag="PK849_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HPE64890.1"
                     /translation="MRTEEIIRLIKESKKKTPAKAYIAGDLEGLDGERITFIGGKDFG
                     ILLGDRDDVERVLVENAGKIRSAHVEISARNSAVPLADLTKYEARIEPGAIIRDMVEV
                     RKGAVVMMGAVINIGAVIGERTMIDMNVVVGGRAEIGADCHIGAGAVVAGVIEPPSAT
                     PVVIEDNVLVGANAVILEGVRVGKGAVVAAGAVVTKDVPPHSVVAGSPAKVIKEVDAK
                     TESKTQIVQDLRTLK"
     gene            5665..6873
                     /locus_tag="PK849_01825"
     CDS             5665..6873
                     /locus_tag="PK849_01825"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPE64891.1"
                     /translation="MIVQKYGGSSVATPEKIKNVAARIKKRVDEGEKLIVVVSAMGKT
                     TNSLISLAQEVSAEPSPRELDMLLATGEQMTSALLAMALSDFGIPALSYNAFQLEMTT
                     TGDFSNARIMDLNLNKLKRQLEMRSVIVVTGFQGITPDGDLTTLGRGGSDTSAVAIAA
                     KCGCPCEIYSDVAGVYTCDPNKRAGARKLEYITFDEMLELSSLGAKVLHSRAVEIAKK
                     YSVDLYCASTFSDERGTYVVNSLPEWLEQPVVTGVTTDSNQMRVNIVRMPASIEAVSV
                     LFGGLAEQGVNVDMISTVNENGHSHLTFTVVDAKEKTVLETVRRTLAPWEGWNVTEDR
                     NVAKISAVGVGMKSAPGVAARFFGALSRKGIKMLGTTTSEIKISALVSREEGDEALRA
                     LVDEFGLGEA"
     gene            6878..7969
                     /locus_tag="PK849_01830"
     CDS             6878..7969
                     /locus_tag="PK849_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE64892.1"
                     /translation="MLSPVYAPYTLVVERAEGVRLFTDKGVFLDTYAGIGVLPLGHSH
                     PDVVRAIREKSERYTHLSNYFLDPDAPALAESLLFMTGRKGEVYFANSGAEAMEAALK
                     AVKKNREGALVSFEGNFHGRTLGALSVTWGPAIRHPFDPLIPHCAFLPFNGEALLRFA
                     RENEIAAVFLECIQGNSGVYPLPADLAEAVNILQKERGILVVADEIQAGLGRTGRYFA
                     YEHFGLRPDIITLGKGIGGGLPLGAAIFCEWSPFASGEHGSTFAPNPVSLAAGKATLA
                     HITPSLLGDVSRKGERLRERLALLSWARGTRGLGLMIGVSAENPAEVKKKAFERGVLL
                     NVTGGGIRFLPALTMSDAEIEEILSLLNF"
     gene            8191..9327
                     /locus_tag="PK849_01835"
     CDS             8191..9327
                     /locus_tag="PK849_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPE64893.1"
                     /translation="MLYRTMPQTGDKISILGFGCMRFPLDAEGKIDEPRSAAMLHSAI
                     DRGVNYIDTAYPYHGGESEPVVGRALEGGYREKVFLATKLPSWMVETPSDMDRLLDEQ
                     LTKLRTDHIDYYLLHSLNADRWGIMKRNDYGKFLERALADGRIRRAGFSFHDQLPLFK
                     EIVDDWPWHFCQIQYNFLDTNYQAGTEGLKYAAEKNIGMVIMEPLRGGNLAKNVPDAM
                     MRIWNESPKKLSPAGWALRWIWDQPEVNVVLSGMTTESDLEDNLSAAEGGAPNSLTAI
                     EREMISRVTTEYRTRMKVGCTACQYCMPCPAGVNIPECFNRYNMAFMFDNLEQAKATY
                     PVFLKPGARASACVKCGACEEKCPQNLPIREHLASVVELLEPGE"
BASE COUNT         1954 a         2650 c         2927 g         1907 t
ORIGIN      
        1 cattggtccg tttgatcgtc ccgtctcttc cggagaagaa aagtcccatg ggcgcgttgt
       61 cgaagaggcg ggttaactcc gcggccctcg aatcggcctg ccgtttgaag gcgaaagaga
      121 ggattcctcc gagcaaagtc agaaaggcga ggagcgtagc ggttgacttc agccatgtcg
      181 ggattaccat gaagacggga ctggaggtcc attgggcgag cgagctgaaa tagaccgaat
      241 cagggtttct cttcatctcg cggaggtgtt tgtcgatagc tgccggaatc gccgggtcgg
      301 aatcccgagg ggcggcgaaa aagaggcgtg tcggattgaa gctgatggct gtgtcttcga
      361 ggttataggc gcgcatgtgg tgcgcgccga agaaacggtt ggtgagcacc gcgtccgtct
      421 ttccgttggc gacggcctgg aaggcgcttt cgaagtcgga aaaggtcacg aaggaaacgg
      481 agaggccaaa atcgcggata atccggtcga agatctcctg ctggatggaa cggtagagca
      541 cggcgacccg tttcccgtcg agatcgggaa tcgagcggat tccgcttccg cggcgtgaaa
      601 aaacctggaa ccagctgtac agcgcagggt cggagtgaaa cgagaagagt tttccgcgcg
      661 cctcagtgta ggccacgtcg ggcataaggt cgatatcgcc cttttcgaga cggtcgagcc
      721 cctgactcca tgttccgtgg acgaaccgga gctcccaccc ctctttttcg gctatgtacc
      781 tgatgatgtc cgcaaaaatg cctgcgggaa ggccggattc cgcagtgaaa atcttcggac
      841 tgttctcgta gaagcctacg cggacaattt tcgctgcaaa gaggtctgtc ggaaaacatg
      901 ccgcacaaag gatgagaaat acaaacaaaa ggagaccgta tacgcgcatc cggaacggcg
      961 gattactctg gcgactgcgt atgctgccca taagaccagc ctcctctttc ttttgttccc
     1021 aaacatagaa tacactactt tcaccgaaag gcaaagagaa gagcagaggg cgagtaactc
     1081 ttttttcttc cccgtcgccc ttaccaaccg ggggggacaa aaaagaccgc cccgggttga
     1141 ggggcggccc tattgtcgtc ggaagagaga gggttaccgc atgttgtact tcttcatgac
     1201 agcttcgtac gagccgtcgg ccttcatatc gtcaagcgtc ttctgaatcg acgctatgag
     1261 ggcgtcgtca gtgtccttgt ggaatgcgta gtagaggtcg ctttcgctga gcgtccagac
     1321 gctctcgtag tcgtcggcat tgaagccaag gctctgaatc tgccatttcg ccacgctttc
     1381 ttcaaaaacc catatatcga tccggccttc ggtcagcttc ttgatgttct ggtcgttcga
     1441 aggaactcgt tcaatctgtt gaatacccgc ctgcttcgcc agttcgtcgc cgatatcatc
     1501 tctgacgacc ccggccttca ggttggccag atcgcttgcg gaagtaattt tcagcccctt
     1561 gcttttcagc gccagcacgc cgaccttcgt cggagcgagc ggtcccaccc atttgaacag
     1621 aggtttgcgt tcgtccgtga acgtcataag gaaaaggcat gtgttcgggg tgttttgtac
     1681 ttcacggtat cctctcgccc acgggagcac ctggatatcc tttacagtct tcgccgcccc
     1741 cagtttctca aagatgccga cgagtacctc aacggacgct cctgtcgccg ttccgtccgc
     1801 tcccgccatg ttgaacggag gataatcctc ggtcatgaac gtaacatcat ccaccgtcag
     1861 cgcccacgcg cttaccgaga acaagcaaaa agccattacg agaaaaacct tcatcaattt
     1921 cactatgttt cccctccttt tggtgtttgg ggcgccggtt tcgattgatt cattcgatcc
     1981 ggagcagtat agaaaaaaga gtgattgagt ggtataaata aaatatatca aaattgctaa
     2041 aaaagtcaag gttttttatc tgtggcgtgg gtgaaaagaa aacgaaacaa ggccgcccgg
     2101 attgaggcgg ccttgtcagg tactatttag aaagcgtttt ttgctattgt acgttgtact
     2161 tcttcatgat tgcttcgtac gcgccgtcgg ccttcatctc gtcgaggacc ttctgcatcg
     2221 actcgataag cgcatcgtcg acgtccttat ggaaggcaaa atagaggccg ctctcgctca
     2281 tacgccacac gctttcgtag tcgtccggat tcagccccat ctccttgacc tggagcttcg
     2341 cgctgctttc tccgaagacc cagatgtcga tgcggccttc gttcagcttc ttgatattct
     2401 ggtcgttgga aggcactctt tctatctgct tgataccggc tttctgagcg agcgcgtcgc
     2461 cgacatcgtc acggatgact ccggccttga ggttgacaag ctcctcggcg gaggcgatct
     2521 tcattccctt gctcttgagg gcgattgcgt cgaacgtgtt gacgacgacc ggaccgaccc
     2581 acttgaacag gggtttgcgc tcgtccgtga gagtcatcag gaagaggcag gtgttcggcg
     2641 tgctctgaac ttcccggtat ccccgggccc agggaagaac ctgaatgtcc ttcgccgtct
     2701 tggccgcgcc cattctctcg aagatggtcg tcaaggtatc cactgccgcg cccgttgcca
     2761 ctccgtccgc ccccgccatg ttgaacggag gataatcctc ggtcatatac gtaatatcat
     2821 ccactgtaag agcccatgcg ctccctgaac aaagacaaaa gaccagtaca agagcaatct
     2881 tcaacaactt cacgacgtat cccctcccaa aattatggaa ttatggtgat tctgatacat
     2941 ggggtaatgg tacggaccaa ttcctttttc ttcgtacaaa gaaaccatca aggcgttgat
     3001 tggaatatat caaatttgct ccaaaagtca agcccggttt caccagttga aaattcatgg
     3061 ttgacaaatc ggcgttcttt gcctaaaatc actcaacata tcgttagatt tgaaaaagcg
     3121 atgaaccgga tcgccttttt ttaaaaagcg tcgcgtacag agaggaacgt tcatcggctg
     3181 aaagacgttc cggcggcgga gaagaaggcc caaagcccgg tgagctggaa tggaaagacg
     3241 agcacggaac ggtgttcgct ggagtacatt cacggtccgg acctccgtta cagtgtccga
     3301 gcgggacata tttcgacatg tccaatcagg gtggtaccgc gagttcaggc tcgtcccttt
     3361 cggggacggg cctttttgta tagggaaccg acactatgat caggaggtgc gagcgatgtt
     3421 tggaggaaca ggtactgcgg tggtaacccc gttcaaggac gggagcgtgg attacgagag
     3481 tttcgagcgg tttttgaact ggcagatcga aggaggcgtc gagttcctcg tcgtccttgg
     3541 gaccacaggc gaaagtccga ctataaccgc cgacgaacgg gcggagatta tcaagttcgc
     3601 cgtgaaggcg gcggcgggca gagtgcgcgt ggttatcggc acggggacga attcgacggc
     3661 gagcacgatt ctgctgtcgc agcaggcgga ggggctgggc gccgacggtt tgctggtcgt
     3721 ggggccgtac tacaacaagc cgacgcagga gggtttctac cagcacttca aggcggtcgc
     3781 tgagagcgtg aaaaccccga tcatcgtcta caacgtgccc gggcggaccg ggtcgaacat
     3841 cgcgccggag acggtgtacc gcctgagcaa gctccgcacg atcgcgggga tcaaagaagc
     3901 ggcgggggat atggcgcaga tcgacatgct cgtgcgcctc atcaagaagg aacgtcccga
     3961 tttcgccatc ctttcgggga acgacgatca ggcgttccac cttgtgaaca gcggcggcga
     4021 cggcgtaatc tcagtgctct ccaacgtgat gccgcgcgag acgtccgaca tgatccgcct
     4081 ggctcttgca ggagaggtgg agaaggcgcg tgcgctgcat cacaggatgt tcccgctcat
     4141 gaagaatctc ttcgtcgagg cgaatccgat ccccgtgaag tacgcggcca gcaggctcgg
     4201 cttctgccgc aacgaacttc gcctgccgct cgttccggcg tcggagaagt gcgttgcgat
     4261 cgtcgacgcc tccatgaagg agtgcggaac ggcgctatga gatacggtct tgtaggcgct
     4321 tcggggagga tgggcggcga gatcaggaag gccttcgccg cgcacgaact tactttttcc
     4381 ctcgacgtcg gcggtgaaac gcacgacgcc gcgccgcagg tgctggtcga cttctccctt
     4441 ccggcggcct tcccctcaac gttcgcggtt gcgcggcgct ttgtctgccc gctggtgatc
     4501 gggacgaccg gtctctccgc ggagcagctc gccgagctga gaacgctcgg ggagcaggtc
     4561 gcggtggtgc agagcttcaa cttcgcgacc ggcgtcacgt tgctgaagat gatgctccgc
     4621 gagttcgccc cgctcttcgc ggactgggac gtcgagatca gcgagacgca ccacaataag
     4681 aagaaggacg ccccttcggg gacggcgatt ctcctccggg aggcgacggg gcgcgactgc
     4741 ccgacgcatt cgctgcgcct cggggggatt cccggagacc atacggtctg ctacgcgaac
     4801 gagggcgagg tccttaccct gacgcaccgg gctctttcgc ggagcgtctt cgccctcggc
     4861 gcgctgaagg ccgcagagtt tgcggtctcg gccgcgcccg gattctattc tttcgaggag
     4921 gtcctctcat gcggactgaa gagataatac ggctcatcaa ggagtcgaag aagaaaacgc
     4981 ccgccaaggc gtacatcgcc ggggatcttg agggacttga cggcgagcgg atcaccttca
     5041 tcggaggaaa ggacttcggc attctgctcg gcgaccgtga cgacgtggag cgggtgctcg
     5101 tggagaacgc ggggaagatt cgctccgcgc acgttgagat ttcggcccgc aactcggccg
     5161 tgcccctcgc cgacctgacg aagtacgagg cgcgcatcga gcccggcgcg attatccgcg
     5221 acatggtgga ggtccgcaaa ggcgctgtgg tgatgatggg cgcggtgatc aacatcggcg
     5281 cggtcatcgg cgaacggacc atgatcgata tgaacgtcgt ggtcggagga cgggcggaga
     5341 tcggcgcgga ctgccacatc ggcgcaggcg cggtggttgc cggagtgatc gagcccccga
     5401 gcgcaacgcc cgtggttatc gaagataacg tgctcgtcgg cgcgaacgcg gtcattctgg
     5461 aaggagtgcg ggtcggaaag ggcgccgttg tggcggccgg agccgtcgtg acgaaggacg
     5521 tgccgccgca ttccgtggtc gccggatcgc ccgcgaaggt gatcaaggaa gtggacgcca
     5581 agacggagtc gaagacgcag atcgttcagg acctccggac gctcaagtga tcgaaccggt
     5641 cggacgaagg ggagagaagc ggaagtgatc gtgcagaagt acggcgggtc gtcggtggcc
     5701 acgcccgaga agataaagaa cgtcgccgcc aggataaaaa agcgcgtgga cgagggggag
     5761 aagctcatcg tggtagtctc cgcgatggga aagaccacga attcgctcat ctccctcgcg
     5821 caggaggtgt cggcggagcc gtcgccccgc gagctggaca tgctcctcgc aacgggagag
     5881 cagatgacct ccgcccttct ggcgatggcg ttgagcgact ttggaattcc ggcgctctcc
     5941 tacaacgcgt tccagcttga gatgaccacg accggcgatt tcagcaacgc ccggatcatg
     6001 gacttgaacc tgaacaagct gaagcggcaa ctcgaaatgc ggagcgtcat cgtcgtcacc
     6061 ggtttccagg ggataacccc ggacggggat cttacaacgc ttggccgggg agggtccgac
     6121 acctcggcgg ttgccatcgc ggcgaagtgc ggttgtccat gcgagatcta tagcgatgtg
     6181 gccggtgtgt acacctgcga cccgaacaaa cgcgccgggg cgagaaagct ggagtacatc
     6241 accttcgacg agatgctcga gctctcctcg ctgggggcga aggtgctcca ctcgcgggcg
     6301 gtggaaatag caaaaaaata ctcggtcgat ctgtactgcg cgtcgacctt ctccgatgaa
     6361 agggggacct acgtggtgaa cagccttccc gaatggctgg aacagcctgt ggtgaccgga
     6421 gtgaccacgg acagcaacca gatgcgggtg aacatcgtac gcatgccggc ctccattgag
     6481 gctgtgagcg tgctcttcgg cgggctcgcc gagcagggag tgaacgtgga tatgatctcc
     6541 actgtcaatg aaaacggcca ctcgcacctc accttcactg tggtggacgc caaggagaag
     6601 actgtgctgg aaacggtccg gaggacgctc gccccttggg aggggtggaa cgtcacagaa
     6661 gaccgaaatg tggcgaagat ctccgcggtc ggcgtcggca tgaagtcggc cccgggcgtc
     6721 gcggcgcgtt tcttcggcgc gctctcccgg aaggggatca agatgctggg gaccacgacg
     6781 tcggagatta aaatctctgc gctcgtctcc cgcgaagagg gcgacgaggc gctgcgcgcc
     6841 ctggtggatg agttcggcct gggggaggca tagccggatg ctctcccccg tctatgcgcc
     6901 gtacactctt gtcgtcgagc gggcggaagg cgtccggctc ttcactgata agggcgtctt
     6961 cctggatacg tacgcgggca ttggcgtcct ccctctcgga cacagccacc cggatgtcgt
     7021 ccgggcaatc cgtgagaaat cggaacgcta tacgcatctg tcgaactact ttctcgaccc
     7081 ggacgcgcct gcgcttgccg aatcgctgct cttcatgacc ggcagaaaag gcgaggtgta
     7141 ttttgccaac tccggcgccg aagcgatgga agccgctctg aaagcggtga agaagaaccg
     7201 cgaaggagcg ctcgtctctt tcgagggaaa ctttcacggg cgtaccctgg gtgcgctctc
     7261 agtgacctgg gggcctgcga tacgccatcc tttcgatccg ctcattccgc actgcgcctt
     7321 tctcccgttc aacggggagg cgctgctgag gttcgcgcgg gagaacgaga tcgcggccgt
     7381 cttcctggag tgcatccagg ggaacagcgg cgtctatccg cttcccgcgg acctggccga
     7441 ggcggtgaac atcctgcaga aagagcgggg catcctggtg gtggccgacg agattcaggc
     7501 gggccttggg cgcacgggca ggtacttcgc ttacgagcac ttcggccttc gccccgatat
     7561 catcactctc gggaagggga tcggcggggg gcttcccctg ggagcggcga tcttttgcga
     7621 gtggtcgccg ttcgcatccg gcgagcacgg ctcgaccttc gccccgaacc ctgtttcgct
     7681 cgccgcaggc aaagcgacgc tcgcgcacat cacaccctct ctgctgggcg acgtctcccg
     7741 gaagggcgaa cgcctccgcg aacgcctggc gcttctctcc tgggcgcgcg ggacgcgcgg
     7801 gctggggctc atgatcggcg tgagcgcgga gaatcccgcg gaggtgaaga aaaaggcctt
     7861 cgagcgcggc gtgctgctca atgtaacggg cggcgggatc cgattcctcc ccgcgctcac
     7921 aatgagcgac gcggagatcg aagagatcct gtcgctgctg aatttctgag gacgaggccg
     7981 ggaagtcgct tcccccatac tccgccttta tcagagaggt tcggaatctg tgccgcttgg
     8041 ggccggattc cggaccttcc tgttattcgg agtactgcgc aacaggaaag tctatgcgat
     8101 aatatatgat atacggaaag atggcgcgcc tgtttttcgt gccggcactt ctctcaggcg
     8161 cggcgaaata tgaaggaggt tgtattgaat atgctctatc gtacaatgcc ccagacaggg
     8221 gataagattt ccattcttgg ttttggctgt atgcgctttc ctctcgatgc cgaaggaaag
     8281 atcgacgagc ctcgctctgc ggcgatgctg cattccgcta tcgacagggg cgtgaactac
     8341 atcgatacgg cgtacccgta tcacggagga gagagcgagc ccgtggtcgg ccgggcgctc
     8401 gaaggcggat atcgcgagaa ggtcttcctc gcgaccaagc ttccctcgtg gatggtggaa
     8461 acgccgtcgg acatggaccg tcttcttgac gagcagttga caaaactgcg tacggaccat
     8521 atcgactact acctgctcca ctcgctgaac gcggaccggt ggggaatcat gaaacggaac
     8581 gattacggca agttcctcga aagagctctc gccgacggac gtatccggcg tgcgggcttc
     8641 tccttccacg accagttgcc gctcttcaaa gagatcgtgg acgactggcc gtggcacttc
     8701 tgccagattc agtacaactt cctcgacacg aactaccagg ccgggacgga agggttgaag
     8761 tacgccgcgg agaagaacat cggcatggtg atcatggagc cgctcagagg aggcaacctg
     8821 gcgaagaacg tccccgacgc gatgatgcgc atctggaacg aatcgccgaa gaagctctct
     8881 cccgcagggt gggcgctccg gtggatctgg gatcagccgg aggtcaacgt ggtgctcagc
     8941 ggcatgacaa cggagagcga cctcgaagac aacctctccg ccgcggaagg gggcgcgccg
     9001 aattcgctca cggcaatcga acgcgagatg atctcccgcg tcacgacgga gtaccgcaca
     9061 aggatgaagg tcgggtgcac cgcgtgccag tactgcatgc cctgtcccgc aggcgtgaat
     9121 atccccgaat gcttcaaccg ctataatatg gcctttatgt tcgataatct tgagcaggcc
     9181 aaggcgacct atccggtatt cctgaagccg ggggcgcggg cgagcgcgtg cgtcaagtgc
     9241 ggcgcctgcg aggagaagtg cccgcagaat cttccgatcc gcgaacatct cgcgtcggtg
     9301 gtcgagctgc tggaacccgg cgagtaaggc cgttttccga tgcggcgtta cgctctgctg
     9361 acggccggaa gccgcttccg cgtgcacatc gtcttccagg ggatcgtcgt cggactttcg
     9421 gcgggcgtcg cctctatc
//
LOCUS       DAOPKU010000033         9375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_7720_length_9375_cov_5.395494, whole genome shotgun sequence.
ACCESSION   DAOPKU010000033 DAOPKU010000000
VERSION     DAOPKU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7720_length_9375_cov_5.395494"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1053
                     /locus_tag="PK849_01840"
     CDS             211..1053
                     /locus_tag="PK849_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPE64894.1"
                     /translation="MKEKEVRYYFAEQRGLEVVRLKGSRFFYPEHSHVSTYTASLVLS
                     GALRLEAGRATTVHGPDSFFVIPPYEPHALSSSGGCETITICMGDDLLRALSGGEKSL
                     NDCLRALSGALPAAPWIFTERFRRMLDRLFSSVREPGRRQRDFVGAVEQRLLLHPEEP
                     LDLETFAEALHVSRFHLLREFKRSVGLPPHKFQMQNRIRKARKMMLDTRLPLTEVALA
                     AGFYDQSHFIRCFRKLAGMTPSEFREACAIGIPSAERGKKLGEEREPLRPRADFVRNP
                     CGEE"
     gene            complement(964..1356)
                     /locus_tag="PK849_01845"
     CDS             complement(964..1356)
                     /locus_tag="PK849_01845"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE64895.1"
                     /translation="MSGMNVFEKFNTGKMRIPGGEREFNSLEWKKHPAFNGVELKHLL
                     TGEQTGGGFSFHLVRIAPGSAIGLHVHETQLETHEVIAGQGVCLNDGTEIPYEEGTVS
                     FFPAGVPHEVRAGTEGLSLFAKFFPALC"
     gene            1615..4251
                     /locus_tag="PK849_01850"
     CDS             1615..4251
                     /locus_tag="PK849_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPE64896.1"
                     /translation="MPLRLKFFLSVVLTLTLVLLMAGFTSNYTTMQAFRNVERRFFQQ
                     DMARVQNRLDAELQMLHHYAADWGAWDSMYFFMDRKDRVRFDEMIDSASLRALDVDLL
                     ALFDTNLLPVVAYTVGPEGEEEALPPALLEDLRRQALLLSDRASEGAFDDILITGDGA
                     FFAGISPVLTTYRTGPSRGLLIVGALLQKRLPLMRESLGTQFDILPLSEDAAPERSSE
                     DIRIAPNESGGASVWQARGSAVIRIDSPRTIYEKGRDAVMASYLWILLAGMGILIVVT
                     LLLETFVLARLKRLQTLSDKIAAEGMIEARVPLEGKDEITALSRSYNTLLHTLENLLM
                     DIPDAFFLVDPEGVIVMSNEAARAALKQDDGADLRGRDIAAFLGREKLDFYRKFDEPA
                     EVGAGEGSVFEASLLRLDGALVPVEVHRRRIEYGDRTLLLLLARDLTERKQFEERLAQ
                     KAYFDAMTGLPNRHSFVRALNRALNAGDALAVAVMNMDHFKLVNAQVGNLNGDRVLMI
                     IAERIRTLLEPPAQLYRTGGDEFAALIPLAGRTEEIASLMERIRRAVAAPCPVGDDSV
                     FPSASIGVVLDIADGMASSEIMDAALHAMARARKNGLGRIAYCRAGDVTANGAGGENI
                     LTLQAELHAALDRDEFIPFFQPVYSIASGRLSGFETLSRWKHPQKGILPPAAFIPQAE
                     QTGFVANLDTRMLEHALDAVNTARRLNPQLRLFFSSNASSVFLRSAAAVESVKFFLES
                     TGADPTRFTLEVTESSLIENLDDVHVKLDLLKESGIRIALDDFGTGYSSLQYINNLPL
                     DVIKLDRAFVSQLFVSAKTERMMRSIITMGRDLGLDIIAEGVETAEQLAWLKSAGCPK
                     AQGYYFSPPVPWDEAQRMIQEV"
     gene            4256..>4477
                     /locus_tag="PK849_01855"
     CDS             4256..>4477
                     /locus_tag="PK849_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64897.1"
                     /translation="MKQRMRSTVRSVFLVCFLTVFLSAPEASGADGRVVIFALNDMHS
                     QLLPVTTKVKTTTVLFGGLAHAGGVIAEQR"
     assembly_gap    4478..4487
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4488..5904
                     /locus_tag="PK849_01860"
     CDS             <4488..5904
                     /locus_tag="PK849_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPE64898.1"
                     /translation="AEKDPVLALQAGDMVAGLMWRLFSGEPEFSALEHAGVQAGTLGN
                     HEFDYGVEHLKKLLSFTSMPMVVSNLEFDDDFLKQRVLKRLMLQAGAMKVGVFGLASP
                     NLFALAGAGDSVKVDAALRQISEKMVAGLREEGADAILLLSRLSDEENEALAAGVEGI
                     HAILGGSSHNELHETRIVEGPNGWKTALGRAGAYCAFVGRLRLAVKDGKLDEQETEWS
                     LLRVTPRARTDGDVLALALEYERKLNEAMLFTIGTFSDAADARAQTVRARESALGNLI
                     ADSLRWRMKTDVAVINGGGVRGDKIFPAGSVSRKVLAEILPFDNEIHIVVLTGEQLRK
                     VFEIAASSLVGKDDRYESSVRTYAGGFLQISGARVVYSLSGRPTLVDPDGKVLEWGNR
                     VKSLHVERDGAWLPVEDDASYTLAANSWTATGGDRFSVLGDAPQQKTDFKDLDVLADY
                     IASHPEGRVRLELDGRITVEE"
     gene            complement(5997..7172)
                     /locus_tag="PK849_01865"
     CDS             complement(5997..7172)
                     /locus_tag="PK849_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE64899.1"
                     /translation="MKAILAGTLETGRGTTQKNATIFLDKGKIVDIAEGLDPKGASEV
                     LDASAFVVTPGLIDAHTHIGTYCEGFPVGMADANDMVHPIAPQLRILDAIYQDDTAFE
                     DALAGGVTCVQTLPGSGNVIGGQGAIIKTSTSVHGRKKVVEEMLVRAPSSMKAALGEN
                     PIRVYTEKGKLPNTRMGNAALMRQALVDAENYRNKQNQAKAKNEAGERNLQHEAMLPV
                     LDGELPLCVHAHRCDDIATAVRIAKEFSIRYTVEHCTEGHLIAPFLAENKVMAAVGPT
                     LTGKPKIELRNKTWDTPVLLWKAGVHFCIITDHPVVPIEHLSVCLSLAVRAGLPREEA
                     LRAVTLSAAEHLGIADRVGSIEKGKDADIVVWDGDPLDARSRAVATIIDGETAYSRI"
     gene            7388..7819
                     /gene="tsaA"
                     /locus_tag="PK849_01870"
     CDS             7388..7819
                     /gene="tsaA"
                     /locus_tag="PK849_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPE64900.1"
                     /translation="MNEEQMQLFHIGVVRSSLLTRADCPKQGGEGGVEAEIFVAPEYR
                     EGLDGLEPGRDVLLLTWLHRAERDVLKVHPRGNREAPLKGVFATRSPARPNPVGLHRV
                     KILELEKERGRLLVAPLEALDGTPVLDIKPVLRESGPFSEA"
     gene            7994..>9375
                     /locus_tag="PK849_01875"
     CDS             7994..>9375
                     /locus_tag="PK849_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64901.1"
                     /translation="MSSTLRRNAVILCMVLFGVLFSWLVVERTDRKMRDDLLYQTRLG
                     VQTIDLDQVRALSGADEDVEKEEYHSLRRRLSAFREAADCRYVYLMQRRPVRDNPAAT
                     EVVFLVEAQNDGIETSPPSQPGDVYEEEYITEEFAALFDGGAPFVEGPVEDEWGIWVS
                     ALVPMVDHRTGKLLAVLGVDYDAKLWRWTVVSSSLLPVSLMLVLLLICIALFVLSRPV
                     DPMPRPVLKRLLPTMAVALLLLSSAAVLLLWRTYSDNLDERLNSLHASISAEFHLDLK
                     NELSLMESSIASLAEAPRLQWALFAGDADALFSDWLSMFARLRREYGVRSFSFANEEF
                     RRLYSFAPGSDEKLFYDGPVVQKTKETGKIASGVEVCPSGNLVLRAVLPVSRAGELVG
                     YAIITKDVQGLLQKRSLRSGSHLALSLRKGLLDRAKWEKTCNPDRTTEWEQFPDCVIA
                     FSSMERLPPG"
BASE COUNT         1692 a         2727 c         3026 g         1920 t
ORIGIN      
        1 gctccatccg cgcggaggcg gtgcagcagc aggggggcgg cggcggaggc ggatgctcgg
       61 tcggcggcgg gagtgcgtgg acggccgcgc tgctgctctt tgttcccctc gtcgtcaacg
      121 gatgcggaaa gcgcagacgg cgctcataag gttgttcgtc gggtacctat gaaatatact
      181 tgacgcgcgt gttcgaaaag gggattcgcg atgaaggaga aagaggtccg ctactatttc
      241 gcggaacagc gggggctgga agttgtgcgc ctgaagggat cgcgcttctt ctacccggaa
      301 cacagtcatg tgtccaccta tacggcgagc ctggttctgt cgggggcgct gcgtcttgag
      361 gcggggaggg caacgaccgt ccacggcccc gactcttttt tcgtgatccc cccctacgaa
      421 cctcacgccc tctcctcctc cggcggctgc gaaacgatta ccatctgtat gggcgacgat
      481 ctgttgcggg ctctctccgg cggagaaaaa tccctgaacg actgcctgcg cgctctttcc
      541 ggcgcgctcc cagccgctcc ctggattttt accgaaaggt tccggcggat gctcgaccgt
      601 cttttttctt cggtgcggga gcctggccgc aggcagcgtg atttcgtcgg tgcggtggag
      661 cagcggctgc tccttcatcc tgaggagccc cttgatctcg aaaccttcgc ggaagccctg
      721 cacgtcagca ggttccatct gctcagagag ttcaaaagaa gcgtcggcct ccctccgcac
      781 aagttccaga tgcagaacag gattcgaaaa gcgcggaaga tgatgctcga tacgcgcctc
      841 cctctgacgg aagtggccct ggccgccgga ttctacgacc agagccactt catcagatgc
      901 ttccggaagc tcgccggaat gaccccctcg gagttccggg aggcgtgcgc tatcggaata
      961 ccttcagcag agcgcgggaa aaaacttggc gaagagcgag agcccctccg tccccgcgcg
     1021 gacttcgtgc ggaacccctg cggggaagaa tgagaccgtc ccctcctcgt acgggatctc
     1081 cgtcccgtcg ttgaggcaga ctccttgtcc tgcgatcacc tcgtgcgtct caagctgcgt
     1141 ctcgtggaca tgcagtccga tcgcgcttcc cggggcgatg cgcacgagat gaaagctgaa
     1201 cccccctccc gtctgctccc ccgtcagcag atgcttgagc tccacgccgt tgaacgccgg
     1261 atgtttcttc cactcaaggg agttgaactc cctctctccg cccggtatgc gcatctttcc
     1321 ggtattgaat ttttcgaaca cgttcatgcc gctcatacga ccgccccttt tccttttctt
     1381 gaagagtcgc cggagttttc tcgtcccggt atgcactatt ctcttcgggg cgcccgcgga
     1441 tggattgtac aaaagtgctc tcttccctca atcgttccgt tgttttcatg ttatattgaa
     1501 gtcaaggaag tttttgttgc ggaacgcctt ctttcggccg ataatagaaa cgcagtggta
     1561 agcgcgtgat cggagagtgc gttccctccg aaatttacgc ggggagaaaa gccgatgcct
     1621 ctacgcctta aatttttcct gtccgtggtg ctgacgctga cgctggtgct ccttatggcc
     1681 ggtttcacgt cgaactacac cacgatgcag gcgttccgca acgtggagcg ccgcttcttt
     1741 cagcaagata tggcgcgggt tcagaaccgt ctggacgccg agcttcagat gttgcaccac
     1801 tacgccgccg attggggcgc ctgggacagc atgtactttt tcatggaccg gaaggacaga
     1861 gttcgttttg acgagatgat cgacagcgcg agccttcgcg cgctcgacgt cgatcttctg
     1921 gcgcttttcg acaccaacct tcttccggtg gtcgcctata cggttggtcc ggaaggcgag
     1981 gaggaggcgc tccccccggc gctgctcgaa gatctgcgcc ggcaagccct ccttctgagc
     2041 gaccgggcct cggagggcgc cttcgacgac attctcatta cgggggacgg cgcctttttt
     2101 gccggaatca gccccgtgct cacgacgtac aggaccggtc cctcccgcgg gctgctgatc
     2161 gtgggcgctc tgttgcaaaa acgtcttccc cttatgcggg agagccttgg cacgcagttc
     2221 gacatccttc ccctgtcaga ggacgcagcg cccgagagat cttcggaaga tatccggatc
     2281 gcgccgaacg aaagcggcgg ggcgtcggtc tggcaggcga gagggagcgc cgtaatccgt
     2341 atcgattccc cgcggaccat ttacgaaaag ggaagagacg cggtgatggc ttcctacctg
     2401 tggattctgc tcgccggtat gggcatactc attgtggtga cgctgctgct tgagaccttt
     2461 gtccttgcgc ggctgaagcg gcttcagaca ttgtccgaca aaatcgccgc cgaagggatg
     2521 atcgaagccc gcgttccctt ggaggggaag gacgagatca ccgctctttc ccgctcgtac
     2581 aataccctgc tgcacacctt ggaaaacctg ctgatggata tccccgacgc gttcttcctg
     2641 gtcgacccgg agggtgttat cgtcatgtcc aacgaagcgg cgcgcgcggc gctgaagcag
     2701 gacgacggcg cggatctgag gggccgggac atcgccgcat tcctcggaag agagaagctc
     2761 gatttctaca ggaagttcga cgagcccgcg gaggtcggcg cgggagaagg ttccgtcttc
     2821 gaggcgtcgc ttctccgttt ggacggcgcg ctcgttcccg tggaggtgca ccggcggcgt
     2881 atcgagtacg gcgaccggac gcttttgctg ctcctcgcgc gcgacctcac cgagcggaag
     2941 cagttcgagg aacgcctggc ccagaaagcc tatttcgacg ccatgacggg gcttccgaac
     3001 cggcactcct tcgtcagggc gctcaaccgt gcgttgaacg cgggggatgc gcttgccgtc
     3061 gcggtgatga acatggacca cttcaagctg gtgaacgcgc aggtcggaaa tttgaacggc
     3121 gacagggtgc tcatgattat cgccgagaga atccgcacgc ttctggagcc cccggcgcag
     3181 ctctacagaa cggggggcga cgagttcgcc gcgctgatcc cgcttgcggg cagaacggag
     3241 gagatcgctt cgctgatgga gcgtatcagg agggcggtcg ctgcgccctg cccagtgggg
     3301 gacgattccg tcttcccgag cgcgagcatc ggcgtcgtcc ttgatatcgc ggacggaatg
     3361 gcgtcgtcgg agattatgga cgccgcgctt catgcgatgg cacgggccag gaaaaacggg
     3421 ctgggccgga tagcgtactg ccgggcgggg gatgtgacgg ccaacggagc aggcggcgaa
     3481 aacatcctca ccttgcaggc ggagctgcat gccgctctgg acagggacga gtttattccc
     3541 ttcttccagc ctgtctacag cattgcgtcc gggaggctct ccggtttcga gaccctctcc
     3601 cgttggaagc atccgcagaa ggggatcctt cctcccgcgg cctttatacc gcaggccgag
     3661 cagaccggat tcgtcgcaaa cctcgacacc cgcatgctgg agcacgctct tgatgcggtc
     3721 aacacggcgc gcaggctgaa cccgcagctt cggctcttct tctcgtcgaa cgcctcgtcg
     3781 gtcttcctga ggagcgccgc cgccgtggag agcgtgaagt ttttcctgga gagcacaggg
     3841 gccgatccga cgcgcttcac ccttgaggtg acggagagtt cgctgatcga aaacctggac
     3901 gatgtgcacg tcaaactcga tcttctgaag gagagcggaa tcagaatcgc cctcgacgac
     3961 ttcggaacgg gctactcctc gctccaatat atcaacaacc tgccgctcga cgtgatcaag
     4021 ctggacaggg cgtttgtcag ccaacttttc gtttccgcga agacggagcg gatgatgcgc
     4081 tcgatcatca ccatgggaag agatctaggg ctcgacatta tcgcggaggg agtggagacg
     4141 gcggagcagc tcgcatggct gaagagcgcg gggtgcccga aagcgcaggg gtactacttc
     4201 tctccgcccg tcccgtggga cgaggcgcaa cggatgatac aggaggtgtg aggtaatgaa
     4261 gcagaggatg cggagtacgg tacggtcagt ttttcttgta tgcttcctga cggtgtttct
     4321 ctccgccccc gaggcgtctg gagccgacgg gcgggttgtt atttttgcgt tgaacgatat
     4381 gcatagccag cttcttcccg tgaccacaaa agtgaagacg accacggtgc tcttcggagg
     4441 gcttgcccat gcgggcggcg tcatcgcgga gcagcgcnnn nnnnnnncgc cgagaaagac
     4501 ccggtgcttg cgcttcaggc cggggatatg gttgccggcc ttatgtggcg gctcttctcc
     4561 ggggagccgg agttctcggc gctggagcac gcgggcgtgc aggcggggac tttgggcaat
     4621 cacgagttcg attacggcgt ggagcatctc aaaaaactgc tctcgtttac ctccatgccg
     4681 atggtcgtct ccaacctgga gttcgacgac gactttttga aacagcgggt tttgaaacga
     4741 ctcatgcttc aggcgggcgc catgaaggtg ggcgttttcg gactggcttc gccgaatctc
     4801 ttcgctctgg cgggcgcggg cgattccgtg aaggtggacg cagcgctgcg gcagatctcc
     4861 gaaaagatgg tcgccgggct ccgggaagag ggggcggacg ccattctgtt gctgagccgc
     4921 ctctccgacg aggagaacga ggcgctggcc gccggggtgg aggggattca cgcaatactg
     4981 gggggctcct cccacaacga gctgcacgag acgcgcattg tcgaggggcc gaacggctgg
     5041 aagacggcgc tcggacgcgc gggcgcttac tgcgccttcg tgggcaggct ccggctcgcc
     5101 gtcaaggacg ggaagctcga cgaacaagag accgaatgga gcctgctccg cgtgacgccg
     5161 cgcgcccgga ccgacggcga cgtgctggcg cttgcgcttg aatacgagcg caagctgaac
     5221 gaggcgatgc tcttcaccat cgggaccttc tccgacgccg cggacgcccg cgcgcagacg
     5281 gtgcgcgcca gggaatccgc cctcggcaac ctgattgccg attcgctccg ttggaggatg
     5341 aagaccgacg tcgccgtcat caacggcggg ggcgtcaggg gggacaagat attccctgcg
     5401 ggaagcgtct cgcggaaagt gctggcggag attctcccct tcgacaacga gatccacatt
     5461 gtcgtcctga cgggcgaaca gctccggaag gtctttgaaa tcgccgcgtc ctccctcgtg
     5521 ggaaaggacg acagatacga gtcctccgtt cgcacgtacg cgggagggtt cctccagatc
     5581 tccggggcgc gcgtcgtcta ctcgctctcc ggccgtccga cgctggtcga tcccgacggc
     5641 aaggtactcg agtggggaaa ccgcgtgaag agcctccatg tggagcgcga cggggcatgg
     5701 ctgcccgtcg aggacgacgc ctcgtacacg cttgccgcga attcgtggac ggcgaccgga
     5761 ggcgacagat tctcggtgct cggcgacgcg ccgcagcaga aaacggactt caaggatctc
     5821 gacgtcctcg ccgactacat agccagtcac ccggaagggc gggtgcgcct cgaactggat
     5881 ggaaggatca ctgtggagga gtagcggcgg ggagcgttct ggagaggccg ggagagaatg
     5941 ataacggggg gcggtctgcg caggcgcggc cgccccccgt tcgtcggatt ccggcgtcag
     6001 attctggagt aggcggtctc gccgtcgatg atcgtcgcga cggccctgct tcgcgcgtcc
     6061 agagggtctc cgtcccagac gacgatatcg gcgtccttcc ccttctcgat cgagccgacg
     6121 cggtccgcga tgcccaggtg ctcggcggcg gagagcgtga cggcccgcag cgcctcctca
     6181 cgcggcagtc cggcgcggac ggccagcgag aggcagacgg agaggtgctc gatggggacc
     6241 accggatggt ccgtgatgat gcagaagtgc actcccgcct tccagagaag cacgggggtg
     6301 tcccacgtct tgttgcggag ctctatcttc ggcttgccgg tgagcgtggg gcctacggcc
     6361 gccatcacct tgttttcggc gaggaagggg gcgatgagat gcccttcggt gcagtgctcc
     6421 accgtgtagc ggatggagaa ctccttcgcg atgcggaccg ccgtggcgat gtcgtcgcac
     6481 cggtgcgcgt ggacgcagag ggggagttcg ccgtcgagca ccgggagcat cgcctcgtgc
     6541 tgaaggttgc gctctccggc ctcgttcttc gccttcgcct ggttctgctt gttccggtag
     6601 ttctccgcgt cgacgagggc ctgccgcatc agcgcggcgt ttcccatgcg ggtgttgggg
     6661 agctttccct tttccgtgta gacgcggatc ggattttccc cgagcgccgc cttcatggaa
     6721 gagggcgccc tcaccagcat ctcctcgacg accttcttcc gtccgtgtac cgaggtgctc
     6781 gtcttgatga tcgctccctg tccgccgatg acgttgccgc tgccggggag cgtctggacg
     6841 caggtgacgc cgcccgcgag ggcgtcctcg aacgccgtgt cgtcctgata gatcgcgtcg
     6901 agaatgcgaa gctgcggagc gatcgggtgc accatgtcgt tggcgtcggc catgccgacg
     6961 gggaaccctt cgcagtaggt cccgatgtgc gtgtgcgcgt cgatgagccc gggagtgacg
     7021 acgaacgcgg aggcgtcgag cacctccgaa gcgcctttag ggtcgagccc ttccgcgatg
     7081 tcgacgatct ttcccttgtc caggaagatg gtcgcgttct tttgtgttgt gccgcgtccg
     7141 gtttcaagcg taccggccag aattgccttc atgaaaagag tacctccttg cgatgccgga
     7201 gcatcggata ctattgatct ttctttattc tacagcatac acggctctgg gaaaaggcaa
     7261 agcaagtata agaagagtac aaagatattg acgaatagtg accaatttcg agtgcgcgat
     7321 acaatacaat gaagaccggg cggggtgtgt gcattctccc ggggataaag gaaaagaggg
     7381 agagaggatg aacgaggagc aaatgcagct gttccacata ggcgtggtgc gctcttcttt
     7441 gctgacgcgg gcggactgcc cgaagcaggg gggcgagggt ggcgtggagg cggagatctt
     7501 tgttgcgcct gaatacaggg aggggctcga cggtctggag ccggggcggg atgtgctgct
     7561 cttgacgtgg ctgcatcggg cggagcggga cgtcctcaag gtccacccgc gcgggaacag
     7621 ggaagcgcct ctgaagggcg tcttcgcgac ccgttctccg gcgcgtccga atccggtagg
     7681 gctgcacaga gtgaagatcc ttgaacttga gaaggagagg ggccgtcttc tggtggcgcc
     7741 tctggaggcg ctcgacggca cgccggttct ggatattaaa cccgtgctcc gggagtccgg
     7801 accattcagc gaagcgtaga tatttttcag gaagtaagaa tgacatggaa gaaaagctga
     7861 tatatatagt gtaaaataga tgtgttcgca aaggttcggt actcgataga cgcaacggaa
     7921 aaagccgatg atttcggagt tttcccccga aggggagatg tgtgtgggag ttccctctgc
     7981 tgcagaaaga acagtgtctt cgacgctccg ccggaacgcg gtcatcctgt gtatggtcct
     8041 cttcggagtg ttgttttcat ggctggtcgt cgagcggacg gacaggaaga tgcgggacga
     8101 tctgctctat cagacgcgcc ttggtgtgca gacgatagac cttgaccagg ttcgcgcgct
     8161 ctccggcgcc gatgaggacg tggaaaaaga ggagtatcac agcctgcggc gccgtctttc
     8221 ggcattccgg gaggctgcgg attgccgcta cgtctacctg atgcaacgca ggccggttag
     8281 ggacaatccg gcggcgacgg aggtcgtgtt tcttgtcgag gcgcagaacg acgggatcga
     8341 aacatcccct ccgtcgcaac caggagacgt ctacgaggaa gaatatatca ccgaggagtt
     8401 cgccgctctc ttcgacggag gcgcgccttt cgtcgaaggc ccggtggagg acgagtgggg
     8461 gatctgggtc tcggccctcg tccccatggt cgaccatcgg acggggaagc tgctcgccgt
     8521 cctcggggtg gattacgacg cgaagctctg gagatggacc gtcgtctcgt cttcgttgct
     8581 gccggtatcc ttgatgctgg tgcttctgtt gatttgcatc gcgctgttcg tcctctcccg
     8641 gcctgtggat cccatgccgc gtccggtgct gaagagactg ctgccgacca tggcggtcgc
     8701 cctgcttctc ctctcctccg cagcggtgct tcttctgtgg cgtacgtatt ccgacaacct
     8761 tgacgagcgg ctgaattccc ttcatgcgtc gatttccgcc gaatttcatc tggacttgaa
     8821 gaacgaactc tcccttatgg agtcctcgat tgcctctctt gcggaggccc cccgtctgca
     8881 atgggcgctc ttcgccggcg acgcggacgc gctcttctcc gactggctgt cgatgttcgc
     8941 ccgcctgcgc cgggaatacg gcgtacgctc tttttccttt gccaatgagg agttccggcg
     9001 tctctattcc tttgcgccgg ggagcgatga aaaactgttc tatgacgggc ctgtcgtgca
     9061 gaagacgaag gaaacgggaa agatcgcctc cggtgtcgag gtctgtcctt ccggaaatct
     9121 ggtgctgcgg gccgtcttgc cggtttcaag ggctggagag cttgtggggt atgccattat
     9181 aaccaaagat gttcaaggtc tcctccaaaa aagatccctt cgttccggat cccacctggc
     9241 gctttcactc agaaaaggcc tgcttgacag ggcgaagtgg gaaaaaacgt gtaaccccga
     9301 ccggacaacg gagtgggagc agttccccga ctgtgtgatt gccttttcat caatggagcg
     9361 ccttcctccc ggtat
//
LOCUS       DAOPKU010000034         9348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_7758_length_9348_cov_4.312924, whole genome shotgun sequence.
ACCESSION   DAOPKU010000034 DAOPKU010000000
VERSION     DAOPKU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7758_length_9348_cov_4.312924"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PK849_01880"
     CDS             complement(<1..409)
                     /locus_tag="PK849_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64902.1"
                     /translation="MFSALFPVHKDGMTDVLPGAETPGSVKNGGDGEKKEQFAKLLFV
                     SLETADDGKNTTTETPGETPLWTNGARSLTALAASLLGENAGQEAAVIADAADEDPSA
                     QITPDDSLTSLFASAFADAKDATAQIPAASETPA"
     gene            complement(431..1093)
                     /locus_tag="PK849_01885"
     CDS             complement(431..1093)
                     /locus_tag="PK849_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtE intracellular region"
                     /protein_id="HPE64903.1"
                     /translation="MPEEEMELERAEGEIAPSPRKKKKKTKGSKLPFLLLVLLMAAGV
                     GAGLHIAGAWDARPLLYASVPKIPWIGNDLAKLTGIPEEYTLTVEQRRRLELQRWNDR
                     LDELERSLKERETALEALSADLEARSISVEKKEAEFTLKENKPPESPEEEKAYVDMLM
                     RTYQEISPRRAALIIEQLREDLAVKMLAAMPQDARASILGRMEAARAARLTERLAAGG
                     SR"
     gene            complement(1093..1674)
                     /locus_tag="PK849_01890"
     CDS             complement(1093..1674)
                     /locus_tag="PK849_01890"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPE64904.1"
                     /translation="MRPNLSGIVRVQGRIEEIERRIRPKKELPEPPKQERFVDVLERE
                     EAKLPLPGASSGVRVPEVSEGWESHLPGLAARYGVDEALVRAVIRMESGGKTDAVSYK
                     GAMGLMQLMPGTAKMLGVEDPFDPVQNLEGGIKYLSQLADKYNGDLTKTLAAYNAGPG
                     RVDAYGGIPPFAETQKYVQSILALYERNSEGDS"
     gene            complement(1671..2126)
                     /gene="fliJ"
                     /locus_tag="PK849_01895"
     CDS             complement(1671..2126)
                     /gene="fliJ"
                     /locus_tag="PK849_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HPE64905.1"
                     /translation="MKEKVDRFERILKTRVKGREEEQLLLAGQKSEEEQIVTRLDTLR
                     SQKERALVSFSMQQGTLISPQDMWFQRKSIDVIEKHICDGDSSLCDVRQEIEATEVRL
                     VEKHREVQIMEKYISSIVEEWRTTLQKNEQTEMDDIAGIRHGSHGRKNQ"
     gene            complement(2200..3528)
                     /gene="fliI"
                     /locus_tag="PK849_01900"
     CDS             complement(2200..3528)
                     /gene="fliI"
                     /locus_tag="PK849_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HPE64906.1"
                     /translation="MTTKTADDELLQILEHRLGSVQLTRINGRIVQVVGLVAESQGPD
                     VRVGDLCSIRYRNSESSLSAEVVGFRGDRVLLMPLGNLKDVGPGCDVLSMERPLGVRV
                     GQALLGRVLDGLGNPLDDKGPIAASDFYPLYAEPPHPLRRKMITSPLPVGVKVIDGML
                     TLGTGQRIGIFAGSGVGKSTLLAMMARYTEADINVIALVGERGREVREFLDRDLGEEG
                     KKRSVLVIATSDQPPLIRLKAALTATAIAEYFRDCGKNVLLMMDSVTRVARAQREVGL
                     AVGEPPTTRGYTPSVFELLPRLLERAGAGEKGSITGVYTVLVEGDDMNEPVADTVRGV
                     LDGHVVLSRKIAARNFYPSVDLLESVSRVMPSIVDEEHLMAAGRVRELLAVYAEAEDL
                     ISIGAYKSGSNPRVDWAITHLQKVRAFLRQSVGEHVPFDVMAQELKSLVP"
     gene            complement(3509..4402)
                     /locus_tag="PK849_01905"
     CDS             complement(3509..4402)
                     /locus_tag="PK849_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HPE64907.1"
                     /translation="MSKFPRANIIRAVRLLPEAVRVGAAAHSVEEEQEERVSSPPAAQ
                     RKEPDELKELQEEVQRLTSALRDAERENRDLAARVTLSEKELAKEKERLAAERREMEA
                     AQKSEYESLRATAAAEGAEEGRKTGHAEGIRTAREEIAREYEQKVSSLVALLEKVHST
                     LAERTEELAALGMPRLIRLWELMLSKMLHREAQVSEGAVFPVLRSLLERLSDRDRILV
                     YLNPEDSEQTAERKDVFGDLLRGVKHLEFIPDANVEKGSCIVETNLGIYDARWQTQLE
                     QIHREIEHLFLEGRKNDDENG"
     gene            complement(4395..5411)
                     /gene="fliG"
                     /locus_tag="PK849_01910"
     CDS             complement(4395..5411)
                     /gene="fliG"
                     /locus_tag="PK849_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPE64908.1"
                     /translation="MAKTGGKGMPGKEKIAILMVALGNEIAAEVYKRLDDTAIEIVTL
                     EVANLRKVTPEQRLSVLKDAQEVLLAREYMARGGVDYARDILERALGPERAHNLLTRI
                     TASLQVRPFDFMRHTDAQQVLGFIQGEHPQTIALIMSYLEPAQAASIIGGLPATMQAE
                     VAKRIARMDRITPEVLREVERVLERKLSTVMGQDFTLAGGIDAIVNIINNADRGTERN
                     IMEHLEENDPELAEEIKRRLFVFEDIIKLDDRSLQRVLREVEMKELSLALKGATEELR
                     SKFFKNMSKRASEMLKEDMDYMGPVRVKDVEESQQKVVNVIRALEDVGEIVISRGGEE
                     DLVV"
     gene            complement(5427..6992)
                     /gene="fliF"
                     /locus_tag="PK849_01915"
     CDS             complement(5427..6992)
                     /gene="fliF"
                     /locus_tag="PK849_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HPE64909.1"
                     /translation="MNWFRQALSRGGLFWSSLRPWQRFSIVGASALVLLALILGVYWA
                     GRPTYEPLYSALEVEDQSAIVAWLRENKVDYTLDPAANAILVPRDLVYETRLSLAQGG
                     LPKGGSVGYEIFDTTKMGLSEFQQKITYIRALEGELERTIRQIDAVDYAKVNIVIPEQ
                     RLFLEEQQPSTASVLVRLRSGMQIGPDHVKSIVHLVSHAVQGLVPENITVVDTAGKVL
                     SDLLDQEFFIYSGAGDGRTVSSVQRELERQQERELESKVRVMLERVFGPGKVVVRVKV
                     DLDFDRRTSATKEYIPGETGRGVIRSQQQMEETYTGTGGPIGGAPGTTTNIPGYALAQ
                     QQAGETEYAKTDTTNNYEITTRETQQVVTPGAILRLSASVLVDDELPPERLGELRALV
                     APAIGLNETRGDQLVINSMRFSTTFADALADQLAAERRMQIIIALVIGAILLAAAVFA
                     GVIWMRRRRIRQAMAPSQEPKRIPSIQELLSSPELLEAQGELAVLEEQLRAYARSKPE
                     EIANLVQDWLSED"
     gene            complement(7003..7323)
                     /gene="fliE"
                     /locus_tag="PK849_01920"
     CDS             complement(7003..7323)
                     /gene="fliE"
                     /locus_tag="PK849_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HPE64910.1"
                     /translation="MDGLKLDLMKMAGIQSLPAVAAVSEKKDTPRGTGLSFEDLLESS
                     MKQVNDLQQDSDKMVNRLATGDVDDISEVVLSAQRAEFALRLITEVRNKLVDAYQQLG
                     RMSV"
     gene            complement(7500..7919)
                     /gene="flgC"
                     /locus_tag="PK849_01925"
     CDS             complement(7500..7919)
                     /gene="flgC"
                     /locus_tag="PK849_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HPE64911.1"
                     /translation="MRMFRSIDVSGSALTAHRLWMDSISSNLANVNTTRTPEGGPYKR
                     RVPVFAEMLDKTIGGYEDIGGVRVTEIAHDNTPPRLAYQPEHPDANAEGYVAFPNVNV
                     VREMTDMLVASRAYEANLAVADAARNMWAGALEIMRG"
     gene            complement(7922..8323)
                     /gene="flgB"
                     /locus_tag="PK849_01930"
     CDS             complement(7922..8323)
                     /gene="flgB"
                     /locus_tag="PK849_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HPE64912.1"
                     /translation="MISDFNWKVMEKTMEGLSRRFESTSRNLANANTPEYARRNVSFE
                     DQLREVVDGPRSLPMTITNEGHIPSGPMAVSAVVPEEIKIYDEKFRLDGNNVDPEREM
                     AVLSQTRMAYNAMSRFVAKKGSLYRLVIGGR"
     gene            complement(8445..9329)
                     /gene="codY"
                     /locus_tag="PK849_01935"
     CDS             complement(8445..9329)
                     /gene="codY"
                     /locus_tag="PK849_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HPE64913.1"
                     /translation="MNYQEGFTVATAKKSTKITSEQNAAELLPQVIDNEPGLKDLLDK
                     TRQVGRALQSRREGTRPDYNKLAKLLCEFSTANVYLINREGKILGHSWVSEYHSEEVS
                     NFLEKGFMPEVFVEKMNQHRETILSETDGYLFDDDNGNAPEKHMLYIPIYGAAERLGT
                     LLLVRFFDPFSMRDLVLAEYLATLVGIEILHERTKNIEERARERLIVQMAMRALSYSE
                     VESVKHIITELGSFEGVVIASKVADRVGVTRSVIVNALRKLESAGIIESRSLGMKGTF
                     IKVLSPLFVEELGVLQGE"
BASE COUNT         1695 a         2835 c         2667 g         2151 t
ORIGIN      
        1 tcgctggggt ttcggaagcc gccgggatct gcgccgtagc gtcttttgca tctgcaaagg
       61 cggaagcaaa caagcttgtg agcgaatcgt cgggggtgat ctgtgcagaa ggatcttcat
      121 cggccgcatc cgcaataacg gctgcttcct ggcctgcatt ctctcctaaa agagaggcgg
      181 caagtgcggt caacgaacgg gcgccgtttg tccacaaagg ggtttccccg ggggtttccg
      241 tggtcgtatt tttaccgtcg tccgcagtct ccaacgaaac aaagagcagt ttggcgaact
      301 gctccttttt ttcgccgtcg cctccgttct ttacgcttcc cggcgtctcg gcgcctggaa
      361 ggacgtcggt cattccatct ttatgcactg gaaacaaggc tgaaaacatg aagccgcacc
      421 tcccgaatcg ttatcgcgac cctccggccg ccagccgctc cgtcagcctt gccgccctgg
      481 ccgcctccat gcggcccaaa atggacgctc tcgcgtcctg tggcatcgcg gcgagcatct
      541 tcaccgccag atcctcgcgc aactgctcga tgatcagcgc cgcccgcctc ggtgatattt
      601 cctggtacgt ccgcatcagc atgtcgacgt acgctttttc ctcttcagga ctctccggag
      661 gcttgttctc ctttaaagtg aactccgcct ccttcttctc gaccgaaata cttcgtgctt
      721 cgaggtcggc ggaaagcgct tcgagggctg tctcccgttc tttgaggctg cgctccagtt
      781 cgtcgagccg gtcgttccat cgctgaagtt caagcctgcg ccgctgctcg acggtaagcg
      841 tgtattcttc cggaatgccg gtcagcttgg cgagatcgtt tcctatccac gggatcttcg
      901 gaacgctcgc gtacaaaaga ggccgtgcat cccacgctcc ggcgatatgc aacccggcgc
      961 cgactccggc cgccatcaag agcaccagga ggaggaacgg cagcttgctc cccttcgttt
     1021 tctttttttt cttccttggt gaaggggcaa tctcgccctc cgctctctcg agctccatct
     1081 cttcttcagg catcagctat ctccttcaga attgcgttcg tagagagcga gaatgctctg
     1141 tacatacttc tgcgtctcgg cgaagggggg aattcccccg taggcgtcaa cccttccggg
     1201 tcccgcgttg tacgcggcaa gagtttttgt aaggtccccg ttatacttat cggccagttg
     1261 cgagaggtat tttatacccc cttcaagatt ctgtaccgga tcgaacggat cttctacccc
     1321 gagcatcttc gccgtccccg gcataagctg catcaacccc attgccccct tgtacgagac
     1381 ggcgtccgtc tttccgcccg attccatcct gatgacggcg cgtaccaagg cttcgtcgac
     1441 tccgtaccgg gctgccaaac cggggaggtg tgattcccaa ccttcggaga cttcgggaac
     1501 tcgcactccg ctggacgcgc ccggaagggg aagctttgcc tcctcgcgtt cgagcacatc
     1561 aacaaaacgt tcctgcttcg gaggctccgg aagctccttt tttgggcgta tccggcgttc
     1621 gatctcctcg attcttccct gaacgcgaac aatacccgag agattcggtc tcattgattc
     1681 ttcctcccgt gagagccgtg acgaatgccg gcaatatcat ccatttcggt ctgctcgttc
     1741 ttctgaagcg ttgtccgcca ctcctcaact atcgatgaga tatatttttc cataatctgc
     1801 acctcgcgat gtttctccac caggcgcacc tccgtcgctt ctatttcctg ccggacatcg
     1861 cagagggagg agtcgccgtc gcagatatgc ttttcaatga catcgatcga cttccgttgg
     1921 aaccacatat cctgggggga aatcaaggtt ccctgctgca tactgaagga gacgagcgcc
     1981 cgctcctttt gggagcggag cgtatcgaga cgcgttacaa tctgctcctc ttcgctcttt
     2041 tggcctgcga gaagcagctg ctcctcttcc cggcctttta ctctggtctt cagtattctt
     2101 tcaaatctat ccactttttc tttcatggcg tcctccggaa tacatcattt tcattccttg
     2161 tttcggcact caacgacttt ttcttgattc atcgcggtct tacggcacga ggctcttcag
     2221 ctcctgcgcc atgacgtcga aagggacatg ttcccctaca gattggcgga gaaaggcgcg
     2281 gactttctgc aggtgcgtga tcgcccagtc gaccctgggg ttgcttccgc tcttgtacgc
     2341 gccgatgctg atcaaatctt ccgcctcggc gtagactgcg agcagctcgc ggaccctccc
     2401 ggcggccatg aggtgttctt cgtcgactat tgagggcatg acgcggctga cgctctcaag
     2461 cagatccacc gacggataga agttcctcgc ggcgattttc cgcgagagca cgacgtgtcc
     2521 gtcaagaacg ccgcggactg tgtcggcgac gggctcgttc atgtcgtctc cctcaacgag
     2581 gaccgtatag actcccgtga tgctcccttt ctcgccggcg cctgcccttt cgaggagacg
     2641 cggcagaagc tcgaagacgg acggcgtgta tccgcgcgtt gtcgggggct cgccgacggc
     2701 caggccgacc tcgcgctgcg ccctcgccac gcgggtgacg gagtccatca tcaagaggac
     2761 gtttttgccg cagtcgcgga agtattcggc gatcgccgtg gcggtgaggg cggctttcag
     2821 acggatgagc ggaggctggt cggacgttgc gatgacaagg acggagcgtt tttttccttc
     2881 ttctccgaga tcgcggtcga gaaactcgcg gacctccctc ccccgttcgc cgacaagggc
     2941 gatgacgttg atatcggcct cggtgtagcg tgccatcata gcgagaaggg tgctctttcc
     3001 cactccggaa ccggcgaaga tgccgattcg ctgacctgtt cccagtgtga gcatgccgtc
     3061 gatgaccttg acgcctacag gaagcgggga cgttatcatc ttgcggcgca gcgggtgggg
     3121 aggttctgcg tagagagggt agaagtcgga ggcggcgatg ggccccttgt cgtcaagcgg
     3181 gtttccgagt ccgtcgagca cgcgcccgag cagggcctgc ccgacgcgga ctccaagcgg
     3241 tcgttccatg gagaggacgt cgcaccccgg gccgacgtcc ttgaggttac ccagaggcat
     3301 caaaagcacc ctgtcccccc tgaagccgac gacttcggcg gagagcgacg actcgctgtt
     3361 cctgtaccgt atggagcaga ggtcccctac gcggacgtcg gggccttgcg attcggcgac
     3421 aaggccgaca acctggacga tgcgcccgtt gatgcgggtg agctgaacgc tccccaaccg
     3481 gtgttccagt atttgaagca gttcgtcgtc agccgttttc gtcgtcattt ttccgtccct
     3541 caaggaacag atgctctatc tctctgtgaa tctgctcaag ctgcgtctgc cacctggcgt
     3601 cgtagattcc caggttcgtc tcgacgatgc agctcccctt ttccacattg gcgtcgggta
     3661 tgaactcaag atgcttgact ccccgaagca aatcgccgaa gacgtctttt cgttcggcgg
     3721 tctgctcgga gtcctctgga ttcaggtaga cgagaattcg gtcgcggtcg ctcaaccgct
     3781 caagaagact gcgaagaacc gggaaaacag ctccttcgga gacctgcgcc tcgcgatgga
     3841 gcattttaga gagcatgagc tcccaaaggc ggataaggcg aggcatgcca agggctgcaa
     3901 gctcttccgt ccgttcagca agggtcgagt gcaccttttc gaggagggcg acgagcgagg
     3961 aaactttttg ttcgtattct cgggcaatct cttcccgcgc cgtccggatt ccttccgcat
     4021 gtcctgtctt tcggccctcc tcggcgcctt cggctgctgc agtggcgcgc agagactcat
     4081 actcggactt ttgcgccgcc tccatctccc gacgttcggc ggcaagacgc tccttctcct
     4141 tggcaagctc cttctcggag agggtgacgc gagcggcaag gtcgcggttt tcccgttcgg
     4201 catcccggag agcggacgta aggcgttgta cctcttcctg aagctccttc agttcgtccg
     4261 gttctttcct ttgagctgcg ggcggcgagg aaacgcgctc ctcctgttct tcctctacag
     4321 agtgagccgc ggcgcccacc ctgaccgctt cgggaagaag acggaccgcg cgtatgatgt
     4381 tggcgcgcgg aaatttagac aaccagatct tcctctccgc cgcgggagat gacgatctcg
     4441 ccgacatcct ccagggcccg gatgacgttg acgaccttct gctgcgactc ttcgacgtcc
     4501 ttgacgcgca ccggccccat gtagtccatg tcctccttga gcatttcgga ggcgcgcttg
     4561 gacatgtttt tgaagaactt ggagcgaagc tcctccgtgg ctcccttgag ggcgagagag
     4621 agctccttca tctccacctc gcggagcacg cgctggagcg aacggtcgtc gagcttgatg
     4681 atgtcctcga agacgaagag ccggcgcttg atctcctcgg ccagttccgg atcgttctct
     4741 tcgaggtgtt ccataatgtt gcgttccgtt ccgcggtcgg cattgttgat gatgttgacg
     4801 atggcgtcga tgcctccggc gagcgtgaaa tcctgaccca tgacagtact cagctttctt
     4861 tcgagcactc tttccacctc ccgaagcact tccggagtaa tccggtccat ccttgcgatg
     4921 cgcttggcca cctcggcctg catcgtggcg ggcaggccgc cgataatact ggccgcctgc
     4981 gcgggttcga gataggacat gatgagcgcg atggtctgcg ggtgctctcc ctgaatgaag
     5041 ccgagcacct gctgcgcgtc cgtgtggcgc atgaagtcga aggggcggac ctgaaggctt
     5101 gcggtgatcc gcgtaaggag gttgtgcgcg cgttccgggc cgagggcgcg ctccaggatg
     5161 tccctggcat agtccacgcc gccgcgggcc atgtactccc gggcgagaag cacctcctga
     5221 gcgtctttca gcacggagag gcgctgctcc ggcgttacct tccgcagatt cgcaacctca
     5281 agggtgacga tctcgatcgc cgtgtcgtcc agtcttttat agacttccgc ggcgatctcg
     5341 tttcccaagg cgaccatgag aatggcgatc ttctcttttc ccggcattcc cttgcctccg
     5401 gttttggcca tggtctcccc cccgtcctaa tcttcggaga gccaatcctg aaccaggttg
     5461 gctatctctt caggcttgct tcgcgcgtag gcgcgaagct gctcttccag aaccgcaagc
     5521 tctccctgcg cctcaagaag ttcgggcgag gaaagaagct cctgaatgga aggaatacgt
     5581 ttcggctcct gagacggggc catcgcctgc cggatcctcc tgcgccgcat ccagatcacc
     5641 ccggcgaaaa cagcggcggc aagaagaatc gctccaatca cgagcgctat gatgatttgc
     5701 atccgccgtt cggccgcaag ctgatcggcc aatgcgtcgg cgaatgtcgt agagaatcgc
     5761 atcgagttga ttacaagctg gtcgccccga gtctcgttca atccgatggc cggagctacc
     5821 agcgcgcgca gttcgccgag gcgttccggc ggaagctcgt cgtccacgag gaccgaggcg
     5881 gaaagacgca gtatcgcgcc cggtgtgaca acctgctgcg tttcgcgggt ggtgatctcg
     5941 tagttgttgg tcgtatccgt cttcgcgtac tcggtctcgc ccgcctgctg ctgagcgagc
     6001 gcgtacccgg gaatattcgt cgtcgtaccg ggagcgccgc cgatgggacc gcctgttccg
     6061 gtgtacgtct cttccatctg ctgctggctg cggatgaccc ctctgccggt ctctccgggg
     6121 atgtactcct tcgtggcgct ggtgcggcgg tcgaagtcga gatcgacttt cacgcggaca
     6181 acgacctttc cggggccgaa gacccgttca agcatcacgc ggaccttgct ctccagttca
     6241 cgctcctgct gacgctccag ttcgcgctga acggacgaga ccgttctccc gtcgcctgcg
     6301 ccggagtaga tgaagaactc ctggtcgaga aggtccgaga gcaccttccc ggctgtgtcc
     6361 accaccgtga tattttcggg aacaagtccc tgcaccgcat gggaaacgag gtgaacaatc
     6421 gattttacat gatccggacc gatctgcatt ccggaacgga ggcgaacgag gacggaggcc
     6481 gtcgagggct gctgctcttc gagaaagaga cgctgttcag gaatgacgat gttcaccttt
     6541 gcgtagtcga ccgcgtcgat ctgacgaatg gtacgttcca actccccttc gagtgcgcgg
     6601 atataggtta tcttctgctg aaactcgctc aaccccatct tcgtcgtgtc gaagatctcg
     6661 tatccgacgc ttcctccttt ggggaggccg ccttgagcaa gactcaagcg cgtttcgtag
     6721 accagatcgc gaggaacgag gatggcgttt gcggcaggat cgagcgtgta gtccacttta
     6781 ttttcacgga gccaggcgac gatggccgac tggtcttcaa cctcaagcgc ggaatagagc
     6841 ggttcgtacg tgggacgtcc cgcccagtaa actcccaaaa tcaaggcaag caggacaagc
     6901 gccgacgctc cgacaatgga gaatctctgc cacggtctta gggacgacca aaaaaggccg
     6961 ccccgcgaga gcgcttgccg aaaccagttc atcggctact tcctacaccg acatgcgtcc
     7021 gagctgctgg taggcgtcga cgagtttgtt ccgtacctcg gtgatcaggc gcagggcaaa
     7081 ctccgcacgc tgcgccgaaa gcactacttc agagatatca tcgacatcac ccgtggccag
     7141 cctgttgacc atcttgtcgg aatcctgctg aagatcgttt acttgcttca ttgaactctc
     7201 caggaggtct tcgaacgaga gtccggttcc ccgaggcgtg tccttctttt cggaaacggc
     7261 agctacagcc ggcagggatt ggatccccgc cattttcatt aaatcgagct tcaagccgtc
     7321 cacttgaatc tcctcctctt cgcggtaaga gttactggaa cgtattttaa cacaacgggc
     7381 aaggttataa caaaaaaggg aggcagtcgt gtgcgccccc cgttccacag gtcatgagtc
     7441 ctgagactct ttcgtaaaga ggagagagat tccccttttc ggaaattcga aaaaaaacat
     7501 caaccgcgca tgatttccag cgccccggcc cacatgttcc gggcagcgtc cgccactgca
     7561 agattcgctt cgtacgccct gctggcaacg agcatgtcgg tcatttctct gacgacgttc
     7621 acgttcggga aggcgacata cccctccgca ttggcatcgg gatgctccgg ctgatacgca
     7681 agccgggggg gcgtgttgtc gtgcgcgatc tcggtcacgc ggacgccgcc gatatcctcg
     7741 tagccgccga tggtcttgtc gagcatctcc gcgaagacgg gtacacgtcg cttgtacggt
     7801 cctccctctg gggtgcgcgt ggtgttcacg ttggcgaggt tcgacgaaat agagtccatc
     7861 cacagacggt gcgccgtgag cgcgcttccg gaaacatcga tggaacggaa cattctcatg
     7921 gtcatcgtcc tcctatgaca aggcgataga gcgagccttt ttttgcaacg aaccgcgaca
     7981 tggcgttgta ggccattcgc gtctgtgaca gtaccgccat ctctcgttca gggtccacat
     8041 tgttcccgtc gagacggaat ttttcatcgt atatttttat ctcctcggga acaactgcgc
     8101 tgaccgccat cggtcccgag ggtatgtgcc cttcgttcgt aattgtcatc ggcagactcc
     8161 gcggaccgtc gacgacctcc ctgagctgat cctcgaagga gacgttacgg cgggcgtact
     8221 cgggggtgtt ggcgttggca aggttgcgcg aagtggattc aaatcgtctc gacagcccct
     8281 ccatcgtttt ttccattacc ttccagttaa aatcactgat catcgccgta tccctccctg
     8341 aaaacgctct ttctcagttt cggctctccg tgctccggac ttgagcaaaa gccggcggtt
     8401 ttccccgccg gctcatatca taccacagac tttcgtcttc ggtgttactc tccctggagc
     8461 acgcccagct cctcgacgaa gagggggctg agcactttga tgaaggttcc cttcatgccg
     8521 aggctgcggc tttcgatgat cccggcgctt tcaagcttgc ggagggcgtt gacgatgacg
     8581 ctgcgggtga cgcccacgcg gtcggcgacc ttgctcgcga tgacgacgcc ctcgaaggag
     8641 ccgagctcgg tgatgatgtg tttgacggac tccacttccg agtaggaaag cgcgcgcatg
     8701 gccatctgca cgatcaggcg ctcgcgggcg cgctcctcaa tgttcttcgt ccgctcgtgg
     8761 agaatttcga tgcccacaag cgtggcaagg tattcggcaa gcacgaggtc gcgcatggag
     8821 aacggatcga agaaacggac gagaagcagc gtacccaggc gctccgcggc gccgtaaata
     8881 ggaatgtaga gcatgtgctt ctcgggcgcg ttgccgttgt cgtcgtcgaa aaggtagccg
     8941 tcggtctcgc tgagtatggt ctcgcggtgc tggttcatct tctcgacgaa gacctccggc
     9001 atgaatccct tctcgaggaa gttggagacc tcttctgaat gatactcgga gacccaggag
     9061 tgcccgagga tcttgccttc tctgttgatg aggtagacgt tggcggtcga aaactcgcag
     9121 aggagcttgg cgagcttgtt gtaatcgggg cgggtcccct ccctgcggct ctggagcgcc
     9181 cggccgacct ggcgcgtctt gtcgagaaga tccttcagcc ccggctcgtt gtcgataacc
     9241 tgggggagaa gttccgctgc gttctgttca cttgtgatct tggtgctttt ctttgctgta
     9301 gccactgtaa atccctcctg atagttcatc cggcgctgcg gatggttg
//
LOCUS       DAOPKU010000035         9288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_7835_length_9288_cov_4.521066, whole genome shotgun sequence.
ACCESSION   DAOPKU010000035 DAOPKU010000000
VERSION     DAOPKU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7835_length_9288_cov_4.521066"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..391)
                     /locus_tag="PK849_01940"
     CDS             complement(92..391)
                     /locus_tag="PK849_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPE64914.1"
                     /translation="MKERSRQSTAPCSALFLSNRKTRLCQQQIANRIALWLKKAGIEK
                     NLTPHGLRHTFATHLYGATSDLLVVQRALGHRDISTTQIYTHLVDGQLEDALERL"
     gene            complement(420..1070)
                     /locus_tag="PK849_01945"
                     /pseudo
     CDS             complement(420..1070)
                     /locus_tag="PK849_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
     gene            complement(1098..1439)
                     /locus_tag="PK849_01950"
     CDS             complement(1098..1439)
                     /locus_tag="PK849_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPE64915.1"
                     /translation="MAVKKTGSPMAAVGEYETVLPFQAVGVKPCIVKDDETSDVGAIL
                     LELAKVGFAVVFVQESFFVGHTRRIEELNDEYETSFIPIPGLKGSEGVGLESIRSSVE
                     RAVGMDIFAVK"
     gene            complement(1429..2442)
                     /locus_tag="PK849_01955"
     CDS             complement(1429..2442)
                     /locus_tag="PK849_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPE64916.1"
                     /translation="MAPAERYAYAVARLRAMSGRLLEEALIQRILDSEDLDSALKVLG
                     ETTYSSWLVELKGGADFDRVIESELLHVYTEVQKFVPDPRLVHLCRIPYDFHNVKVLL
                     KSLILAREGGERRFDLLTSLGVLPTDDLIMAMESEDYRLLPFGLHRLVPLCLSHWEQT
                     KDILQVENILDDGLFSVLSEMAAATGFDSATRWVKGRIDAENIRNLLRLKRMEMDTGN
                     IASFLHGGGWISREKLLALLPEPVESWPRILSFADVASAFSHIQDLSDLGVLLVEMEK
                     VLDEYVSSILARAKYDAFSPENVIAFLWMKELEAKNLRIAMVSVANNTDRNLARGLLR
                     HGR"
     gene            complement(2459..3040)
                     /locus_tag="PK849_01960"
     CDS             complement(2459..3040)
                     /locus_tag="PK849_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HPE64917.1"
                     /translation="MSLADIKKKIENDARDEAGRILEKARENVEAIKKNAADEIKKME
                     NGYSDRFKKEQPEILRRREIVAGLDVKKIELGVRQQAITDAFQAALKTLASMPGEAYI
                     SFIGKLFKKAVETGNEKVYISKSEKNISSEWISKLNAQNGWSLSLSEERRDISGGFIL
                     EQDNIETNCSFDMLVRWIRDEIEADVVKRLFSA"
     gene            complement(3062..3541)
                     /locus_tag="PK849_01965"
     CDS             complement(3062..3541)
                     /locus_tag="PK849_01965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPE64918.1"
                     /translation="MDLLGIMLAILGAAMAAGWAGAGSAIGVGIAGEAGAGVMTEDPG
                     KFGLVLLLQALPGTQGIYGLLIAFFAILKVGLLGGGAGVTVNVWQGLGIFFACLPIAV
                     GGFFSAIAQGKTSAACIQMIAKRPEETGKAVILPAMVETYAVLALLMSIILLNGITL"
     gene            complement(3587..5599)
                     /locus_tag="PK849_01970"
     CDS             complement(3587..5599)
                     /locus_tag="PK849_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HPE64919.1"
                     /translation="MAVARIKKAELYVHKADLDNVLHALQKTGSFEVVPFGSESLSES
                     TAPVHTDLSRVEGLLSEARFLLRFLEPYFKDNVSSLARTMGERETISLHDGKTLEDRT
                     DLMSLSASVRSLERRLMEIRSELTQIASTETLLNALSDFPYPFELLTSGTERVRGILG
                     TMPVSKIPAWREETEALLLSMGEVFTSTQGEKDTEGWIAVFFDEQKTAQITDLLARNS
                     FSRVDVPLSLKKTPEEERLALSDRKVALASEEERVLAEIAGAAEQWVPSVRILSDYWT
                     VLRDRYQAISSGSSTEQVIMLRGWVPASAVQRLTKAITPFGLSVELFLFDPKPEDSPP
                     SHIVNSAWCFPFENLTRLYGAPKYGEIDPTPLLAPFFFIFFGMCLGDAGYGAIMIGFF
                     LWFLRKYKKMPESFKGFFKLFVLSGVSTIVVGALTGSWFGDLVDAFSIFAFLRPLKNF
                     MLVLNPMENPMIFLAISLALGILQLFFGLFIAFYDALRKGNYMGAFADTGGWIVFLTG
                     LLLWGGVASGFISAQTSFLAKTLTFLGAAVLIATQGREKEGIIGKAVSGLLSLYSVTS
                     YLGDVLSYSRLLALGLATSAVGVIINMLAGLAGDVPYIGWIIALLLLIGGHIFSIAVN
                     VLGAFVHSLRLQYVEFFSKFYTGGGTVFSPLTYKTAFVAIKNEPEE"
     gene            complement(5580..5912)
                     /locus_tag="PK849_01975"
     CDS             complement(5580..5912)
                     /locus_tag="PK849_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HPE64920.1"
                     /translation="MAANLAEEIKEVEARSKDIVKDAKNGAAQMINEAQNEVERRTKE
                     AKQKAFRLFKENTASVEREAEEKAKQSVEAGQKKSEQLAKQYGQKVNSAAQWISEEVI
                     SRYGSSEN"
     gene            complement(6125..6200)
                     /locus_tag="PK849_01980"
     tRNA            complement(6125..6200)
                     /locus_tag="PK849_01980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6165..6167),aa:Gly,seq:ccc)
     gene            complement(6389..7765)
                     /gene="rlmD"
                     /locus_tag="PK849_01985"
     CDS             complement(6389..7765)
                     /gene="rlmD"
                     /locus_tag="PK849_01985"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPE64921.1"
                     /translation="MIRVYEKGASVLLRTEKISSDGSGIARTPEGFVIFIPGALPGET
                     LKATVMLRKKEYAIAFPDELLEPHPLRRQPFCPVFNSCGGCQLQHASYALQLELKRAI
                     VQDAFERIYKRPFPPVTSCNPSPEQIRYRNKTSLPVRNREGKTVMGYFARRSHDVVPI
                     KSCPVAAHSIDRAFSAVAESLPVLGLVPYNEKNGKGMLRHAIFRQSIENNDTLVSFVL
                     ADTLSSRKKAIFEERLLPALQEALPNLRSLTLNINSARNNVIVGPKTEVLFGDGLIEE
                     TLASFRFEYDTTAFFQVNSRQACILYEFVVDLAGLTGTERVLELFSGIGTLTSFLAAR
                     SLHVTAVEEWDSSVRMMRSNLSRNGVGEKTAIIAGAVEKVAPSLEGTFDVIVLDPPRT
                     GCDASVLHKVLSLNPRRIIYVSCNPATLARDAGILTDQGFVLDTLTCFDLFPQTVHVE
                     TVARFFRI"
     gene            complement(7975..8538)
                     /locus_tag="PK849_01990"
     CDS             complement(7975..8538)
                     /locus_tag="PK849_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE64922.1"
                     /translation="MASRYEIRFAGSGGQGVILAAVVAGEAAALFEEGLSVVQTQSYG
                     PEARGGKSKAEVVISTEPIDYPKTMKPNLQVILTQQAAEEYAAETLPGGRIIYDDFFV
                     TSFPQVDANIYYLPIVRTAREKIGKEIVTNMVALGAVARVLELEKIVRPESLKKAILA
                     KVPEGTKQLNAQAFDEGYLLFKNSVHL"
     gene            complement(8528..>9288)
                     /locus_tag="PK849_01995"
     CDS             complement(8528..>9288)
                     /locus_tag="PK849_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPE64923.1"
                     /translation="HGIIMHSLLRALVDLKKKKTETVIASGIGCSSRMVGYIDACTVH
                     TTHGRSLAFATGIKLANPELTVVDVMGDGDCTAIGGNHFIHACRRNIDITAIVMNNNI
                     YGMTGGQVSPTTPVGSLATTSPYGAIDPSFDICKLAEGAGASYVARTTIANPKQAEQF
                     IKAGINNKGFSVVEILTHCHTQFGRRNKRRTPIDNVNFFKANSVPLNKAQEMSPEELA
                     EKIVVGEFVKREIPEYTAQYLSIIERVGGLKNGK"
BASE COUNT         2310 a         2391 c         2224 g         2363 t
ORIGIN      
        1 cataacggca tacggcgcaa ttttaatatg caccccggga acgaaaaagg agcagcagga
       61 gccgctccgg atgttcaggc catggatgcg gtcaaagccg ttcgagcgca tcctcgagct
      121 gaccatcaac aaggtgggtg taaatctggg tcgttgaaat atcccggtgc cccagcgccc
      181 tctgcacgac aagcaggtcg ctggtggccc cgtaaaggtg ggttgcaaag gtatgccgca
      241 gaccatgcgg agtcaggttc ttttcaatac cggccttctt cagccacaag gctattcggt
      301 tggctatttg ttgctggcaa agacgtgttt ttctattgga cagaaacagt gcgctgcatg
      361 gcgctgtgct ttgccgggat cgttctttca aatatctttt cagcaggatg cggaggatgt
      421 cctgttgtca tcggaataac aggaggaaga cgcttcgcaa cgggtcgtgc ttttcgaacc
      481 ggccaccttt gaagcattgt tacgttcttt ttctcgccgt tctgttcaag aaccgcgaca
      541 acttcctcta cagtgaaaga gcctttttca atcttgacta ctttgccacc caagcctacc
      601 ggcaccatga tgcggtgctc cacgagaacc gactcctgca caacgccaag aatatcgccc
      661 tgttccactg cgtcgcccgc ttttactttc ggggtgaact cccatttttt ctttctgtcg
      721 atggcgggta cgtcgatacc tcgggagata taatgactct ttgccgcgtc ttctatcaga
      781 ttcaaaggtc tctgtacgcc gtcgtagaac tgttctataa ggccgggccc cagttcgacg
      841 ctcaaaggtt cccctgtact gacaaccctc tctccgggca taaggccgga ggtctcttca
      901 tacgcctgta tagatgccgt atcgccctta agctcgatga tttctccgac gagtccgatc
      961 tcgccgatcc gcaccacgtc gtacatgccg gcgccgaaca tacctttcgc cactacaagg
     1021 gggccggata ttttttcgat agttcctttt atgactttct cagcggccac aatctatcgc
     1081 ctccaaactt ctattcatta cttcactgca aaaatatcca ttccgactgc tcgctcgacg
     1141 ctgcttcgaa tggattccaa accgactccc tcgctccctt ttagcccggg gatggggatg
     1201 aaacttgttt cgtactcgtc gttcagttct tcgatcctac gggtgtgccc gacgaagaaa
     1261 gactcttgga cgaagacgac cgcaaaacct acttttgcaa gctcaagaag aattgcgccg
     1321 acgtccgagg tttcatcatc cttgacaatg cacggcttca ctccgaccgc ttgaaaagga
     1381 aggactgttt cgtactcgcc gacagcggcc attggagaac ctgttttctt aacggccatg
     1441 acgaagtaac cccctcgcaa gatttctgtc cgtattattc gctacagaga ccatcgcaat
     1501 acggagattc ttagcctcaa gctccttcat ccaaaggaag gcgatcacat tttccggcga
     1561 gaacgcatcg tattttgctc ttgcgagaat gcttgacaca tactcgtcaa gaactttttc
     1621 catctccacc aagaggacgc caaggtcgga aaggtcctgg atatgtgaga aagcgcttgc
     1681 cacgtccgcg aaagaaagaa ttctcggcca gctttccacg ggttcaggaa gcagagcgag
     1741 gagtttctct cgcgatatcc agccgcctcc atgcagaaat gatgcaatat tccccgtgtc
     1801 catttccata cgctttaacc gaagaaggtt acgaatattc tcagcgtcga tcctgccctt
     1861 tacccaacga gtagcggagt caaaacctgt cgctgccgcc atttcggata agacagagaa
     1921 aagaccgtcg tcaagaatgt tctcgacttg aagaatatct ttcgtctgtt cccaatgcga
     1981 aaggcataac ggaacaagac gatgaaggcc aaaggggaga agacggtagt cttcgctctc
     2041 cattgccata atcaaatcat ccgtggggag aacgccaaga gaagttaaaa gatcaaaccg
     2101 gcgctctccg ccttcccgtg caagaatcaa actttttaaa agtactttca cattatgaaa
     2161 gtcataagga atacggcaca gatgcacaag acgaggatcg ggcacgaatt tctgcacttc
     2221 ggtatacaca tgcaaaagct cggattcgat tactctgtcg aagtcagctc cgcctttcaa
     2281 ttcaacgagc caagatgagt atgtcgtctc gcccagaact ttcagtgctg aatcgaggtc
     2341 ttcactgtcc agtatccgtt ggatcaacgc ttcttctaga agacgtccgg acattgcccg
     2401 gagtcgcgcg accgcatagg cataacgctc agccggagcc aaggagcgtc acctccgatc
     2461 acgccgaaaa cagccgcttg acaacgtctg cctcgatttc gtcccgaatc caccggacaa
     2521 gcatatcaaa cgaacagttt gtctcgatat tgtcctgttc cagtatgaat ccacccgata
     2581 tatcccggcg ttcctcactc agagaaagcg accatccatt ctgggcattg agcttcgaaa
     2641 tccactcgga agagatgttt ttttcggact tcgaaatata cactttttca tttcccgtct
     2701 ccactgcttt tttgaacagc ttgccgataa aagaaatata tgcttctcca ggcatggacg
     2761 cgagcgtctt cagtgctgct tgaaaagcat ccgttatagc ctgctgcctg actccaagtt
     2821 cgattttttt cacatcaaga ccggcgacga tctctcgtct tctgagaatc tctggctgtt
     2881 cttttttaaa acggtcgcta tagccgttct ccattttttt aatctcgtca gcagcgtttt
     2941 tcttaatcgc ctcgacgttt tctctggctt tttccagaat tcttcccgcc tcatcacgcg
     3001 cgtcattttc tatttttttc tttatatctg ccaaggacat tcagcattcc tccttgacaa
     3061 tctacagagt gattccgttg aggaggataa tgctcatgag aagtgcaaga acagcgtagg
     3121 tttccaccat cgcgggaaga atgacggctt ttcccgtctc ctcggggcgt tttgcgatca
     3181 tctgaatgca ggccgcggat gtcttcccct gtgcaatcgc cgaaaagaaa ccgccgacag
     3241 caatgggaag gcaggcaaag aaaatgccga gtccctgcca tacgttcacg gtaactccag
     3301 cgccgccgcc aagaagacca actttaagaa tggcgaagaa ggcgataaga agaccgtaga
     3361 tgccctgagt gccgggaaga gcctggagaa gaagcacgag accaaattta ccggggtcct
     3421 ccgtcataac tcctgctcct gcttctcctg cgattccaac accgattgct gaacctgcac
     3481 cagcccatcc agccgccatt gccgctccaa gtattgccaa catgattcca agaagatcca
     3541 tagttataga cacactcctc tcgtattcgt gaaaaatctg aacagtttat tcttcgggtt
     3601 cattttttat tgcgacaaat gctgtcttat acgtcagagg agaaaagact gttccccctc
     3661 ctgtatagaa tttgctaaaa aactcgacat actggagacg cagagagtga acaaaagcgc
     3721 ccagtacgtt gacagctata ctgaaaatgt gccctcctat gagaagaagg agtgcgatga
     3781 tccagccaat atagggcacg tcccccgcaa gaccggcgag catgttgata attacgccta
     3841 ccgcagaagt tgcaagccct agtgcaagca gacggctgta gctcaatacg tcgccaagat
     3901 acgatgtgac gctgtacaga ctcagcaatc cggaaaccgc ttttccgata atgccttctt
     3961 tttctctccc ttgtgttgcg atgagcaccg cagctccaag gaacgtaagc gttttcgcaa
     4021 ggaaagatgt ctgagcggag ataaaaccgc ttgctactcc tccccaaaga agcagtcccg
     4081 taagaaagac aatccaaccg cccgtgtccg caaaggcccc catatagttc cctttccgca
     4141 gagcatcgta gaatgcaata aagagtccga agaaaagctg gagaatgcca agtgcgagtg
     4201 aaattgcaag gaaaatcatg ggattctcca tggggttaag gacgagcata aagttcttta
     4261 gcggacgcag gaaggcaaaa atggaaaaag cgtctacaag atctccgaac cagcttccgg
     4321 taagcgcccc aaccacgatt gtggaaacac cggaaagaac gaagagtttg aagaacccct
     4381 taaaactctc gggcattttc ttatactttc tgagaaacca caagaagaat cctatcatga
     4441 tagctccgta accggcatcg ccaagacaca taccgaagaa gatgaaaaag aacggtgcta
     4501 gcagcggtgt ggggtcaatt tctccgtact tcggcgcgcc gtagagtctc gtcaggtttt
     4561 caaacgggaa acaccatgcc gagttgacta tgtgtgaggg cggtgagtct tcaggtttcg
     4621 gatcaaacag aaagagttct acagataaac cgaagggggt aattgccttc gtgagtctct
     4681 gaacggcaga tgcgggaacc cagccgcgaa gcatgattac ctgttctgtg gaagaaccgg
     4741 aagaaattgc ttggtacctg tcacgcaaga cagtccagta atcactcaaa atacgcactg
     4801 agggaaccca ctgttccgcc gcgccggcaa tctctgcaag aacgcgttct tcttcagatg
     4861 ccaaggcgac ctttctgtcc gagagagcga gtcgttcctc ctctggtgtt tttttgagag
     4921 aaagaggtac gtccactctg ctaaatgagt tgcgagcaag aagatctgtg atttgcgccg
     4981 tcttttgttc gtcgaaaaag acagcaatcc acccttccgt atccttttct ccctgagttg
     5041 aggtaaaaac ctcacccata gataaaagaa gcgcctcagt ttcttctctc caagccggaa
     5101 ttttcgaaac aggcatcgtt cccagaatgc cgcgcacgcg ttcggttccg gaggtgagaa
     5161 gttcaaacgg ataagggaag tcggagagcg cgttcagaag cgtctccgtc gatgcgatct
     5221 gtgtgagttc agagcgaatt tccataaggc ggcgttcaag cgatctcaca ctcgcagata
     5281 ggctcatgag atctgttctg tcttcaagag tttttccatc gtgcaacgag attgtttctc
     5341 gttctcccat ggttctagcc aaggaagaaa cgttgtcctt gaaataaggc tcaaggaatc
     5401 gaaggagaaa acgcgcttcg ctgaggagtc cctccacccg tgataagtca gtgtgaacag
     5461 gcgcggtact ttctgacaat gattccgacc cgaaagggac aacttcaaag gatcctgtct
     5521 tctgaagagc gtgcaggaca ttgtccaaat cggctttgtg aacgtataac tcggcttttt
     5581 taattctcgc tactgccata tcgagaaatc acctcttccg aaatccattg tgccgcgcta
     5641 tttaccttct gtccgtactg cttggcaagt tgctcggact tcttttggcc ggcctcaaca
     5701 gactgttttg ctttttcttc cgcttctctt tccacagagg ctgtattctc tttgaaaagc
     5761 ctgaatgctt tctgttttgc ctccttggtc cggcgctcaa cttcgttctg agcttcgttg
     5821 atcatttgag cagccccgtt ctttgcatcc ttcacaatat cttttgaacg agcttcgact
     5881 tccttgattt cttcagccag atttgctgcc atataaaccc acctccttcc ttggattgaa
     5941 cgcgagtgca cacgctgtgc acacggtcaa cataaaaaca gtgactattt tattaccaga
     6001 caaaatttgt gtcaaacgta acaatatgtc cagatcataa tcaacaaaac gaaacaaggc
     6061 aagagtctta aagaatattt tctttcagga gaaaagtcag tatcagggga ttggggaaaa
     6121 gaattggagc ggacaacggg attcgaaccc gcgaccctta gcttgggaag ctaatgctct
     6181 accagctgag ctatgtccgc cctcttttgc ttgaacgatg gtattatatc cgtcgtatcc
     6241 tgttttggca acatcttttt cctgacacac ctgatttcat ttgttgaaac acagactgga
     6301 gaggatattc tcagtagtat gcgttcttag aaataactgc gttgcccggg taacgttttc
     6361 cctctggcca atgaaaaaca cgtggtgatt agatacgaaa gaatcgcgcc acggtttcga
     6421 cgtgaacggt ctggggaaat aaatcgaaac atgtcagagt atcaaggacg aaaccttgat
     6481 cagtgagaat accggcatcc cttgccaggg ttgccggatt acacgaaaca tagataatcc
     6541 ggcgtggatt caacgaaagg accttgtgca gcaccgaagc gtcacagcct gtgcgcggag
     6601 gatcaaggac aatcacatcg aaagtacctt cgagagaagg cgctactttc tccactgctc
     6661 ctgcaataat cgcggttttt tcgcctactc cattgcggga gaggttgctc cgcatcattc
     6721 gtaccgaaga gtcccactcc tccaccgctg ttacatgaag acttctggcg gcgagaaaag
     6781 aagttaaagt acctataccg ctaaagagtt cgagcactcg ttcagtgccg gtgaggccgg
     6841 ctagatccac aacgaattca tacaggatgc atgcctgacg cgagttgact tggaagaatg
     6901 ccgttgtatc gtactcaaat cgaaaagagg cgagggtttc ttctataagg ccgtctccga
     6961 agagcacctc tgttttcggg ccgacgatta cattgttccg tgccgagttg atgttcagag
     7021 tcaggctgcg taagttggga agcgcttcct gaagcgccgg gagcaggcgt tcttcgaaaa
     7081 ttgctttctt gcgggaagag agtgtatcgg caaggacgaa ggaaacaagg gtgtcgttgt
     7141 tttcgatact ctgacggaaa attgcatggc gaagcatgcc ttttccgttt ttctcgttat
     7201 atggaacgag accgagaacc ggcagacttt cggccaccgc tgaaaaggcc ctgtcaatag
     7261 agtgcgcggc aacgggacaa ctttttatcg gtactacgtc gtgacttctt cttgcaaaat
     7321 accccataac agtttttcct tctcggttcc gtaccggaag ggaagtcttg tttctgtacc
     7381 ttatctgctc tggactcgga ttacatgagg taacgggggg aaacggtctt ttatagatac
     7441 gttcaaaagc atcttggaca atagcacgtt tcagttccag ctgaagagcg tacgaggcat
     7501 gctgtaactg gcatcctccg caagaattga aaaccgggca gaagggctgc cgacgaagag
     7561 ggtgcggttc aagcagttcg tcagggaagg caatcgcata ctcttttttt cgaagcatga
     7621 ctgtagcttt tagcgtttct ccagggagcg cccccgggat gaaaataaca aaaccttccg
     7681 gagtgcgcgc aattccgctc ccgtcgctgc ttatcttttc agtccgtaga aggacggaag
     7741 cgcctttctc gtacactcgg atcatctcct ctttttttgc gcacagacca cattatacac
     7801 ggaagagagc ggaaaaagtc tttcgagtga gggaaaggtt cagagcagaa aaaggcttcc
     7861 aagtttcgtt gggcgtattc ctcgctatta cgttgtattt ggtgtggaga aaggctcaaa
     7921 aattgaacgc cctccttacg aaaagaaggg cgttcattat ggagaaagtg ttttttaaag
     7981 gtgcacgctg tttttgaaca ggagataccc ctcgtcaaaa gcctgggcgt tgagttgttt
     8041 tgttccttcg ggaaccttcg caagaatcgc tttctttagg gactccggtc gcactatttt
     8101 ttcgagctca aggacgcggg ccactgctcc caaagctacc atgttcgtaa ctatttcttt
     8161 gcctatcttc tcacgcgcag tcctgacaat gggaagatag taaatattcg catccacctg
     8221 cgggaagctg gtgacgaaaa aatcatcata aataatacgc ccgccgggga gtgtttccgc
     8281 tgcgtactcc tctgctgcct gctgcgtgag aataacttga aggttgggct tcattgtttt
     8341 cgggtagtca atcggttctg tggagatgac tacttccgct ttcgacttgc ctcccctggc
     8401 ctccggaccg taagactgcg tctggacaac gctcaacccc tcctcgaaaa gagctgcggc
     8461 ttctcctgcg acgacggctg cgagaatgac gccctgtccg ccggaaccgg cgaaacggat
     8521 ttcgtaccta cttgccattt ttcagccccc ctactctttc gattatggag agatactgcg
     8581 ccgtgtactc cggaatctct cgtttgacga attcgccgac gacgattttt tcggcgagct
     8641 cctcgggaga catctcctgt gccttattca aaggcacaga attggccttg aagaaattca
     8701 cgttatcaat aggtgttctc cgcttgtttc ttctgccgaa ttgagtatgg cagtgcgtca
     8761 ggatttcgac gacagaaaat cccttattgt ttatgcctgc cttaataaac tgctctgctt
     8821 gtttcgggtt ggcaatggtg gttcttgcaa cgtatgatgc tccggcgccc tcggcgagtt
     8881 tgcaaatgtc aaaggaagga tcaattgcgc cgtaggggct cgtcgtagct aaacttccga
     8941 cgggcgtggt gggcgaaacc tggccgccag tcataccgta gatgttattg ttcataacga
     9001 tggcggttat gtcgatattt ctcctgcacg catggatgaa gtggttaccg ccaatagcgg
     9061 tgcagtcgcc gtctcccata acatcaacaa ctgtgagctc cggatttgcg agtttgatcc
     9121 cagttgcgaa agcgagggag cggccgtgcg tggtgtgtac ggtgcatgcg tcaatgtagc
     9181 cgaccattct gctcgaacac cctattccgg acgcaatgac cgtttccgtt ttctttttct
     9241 taaggtcgac cagcgccctt aaaagagagt gcataatgat accgtgtc
//
LOCUS       DAOPKU010000036         9259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_7862_length_9259_cov_4.938288, whole genome shotgun sequence.
ACCESSION   DAOPKU010000036 DAOPKU010000000
VERSION     DAOPKU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9259
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7862_length_9259_cov_4.938288"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PK849_02000"
     CDS             <1..1061
                     /locus_tag="PK849_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE64924.1"
                     /translation="GNRKVNRILAIDDKADNLVVLRAMMKMLLPECVLLQALSGEEGL
                     RIAKEQRPDTILLDIFMPGMDGYETTMRLKADPETASIPVILLTAAENTAANRTAGLK
                     VGADAFLSKPIEEEELVAQVRAMLRVKESETALKTKMDVLEDLVEQRTKELKESANRL
                     ALTLDQTISVLANTVETKDPYTAGHQVRCASLALALGNELRLDPQQLRGIHMAARIHD
                     IGKIKIPSEILSKPGKITPLEFSLIKEHPGVGEAIVAPIVFPWPVGRMIAQHHERIDG
                     TGYPKGLFGDDILMEAKVIAVADVVEAISSYRPYRPALGIDAALQEIENGIGTKYDPD
                     AVRACTALFRENRFEFDE"
     gene            complement(1122..2867)
                     /locus_tag="PK849_02005"
     CDS             complement(1122..2867)
                     /locus_tag="PK849_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPE64925.1"
                     /translation="MFTLKKKIVAVISLAVMLAFGISWLLLYGVVRNGIVRQSTLELS
                     QQTALLAASLKDGGMERFTANVEKWEDILRGRVTLIGANGVVLADTERSPEAMENHLT
                     RPEVKGALASGEGSALRYSATTNMYYLYFARKVEAEGRVAVLRAAYPLEFLSEAFAET
                     RNKFIFYLFVAVVLIVAFGTWLARLFFRPLDRIVEFAGKIAQGEAVRFPIMAEPELQR
                     LSSALDGMSARLRGALAELRTEREDLSRIVTALPVGVVLLDEERRVRYLNDVGKRLLC
                     VKEEAPHGVPVERILPSGDMYPLVEAAMNGEEDCLTLDLPELDKRYLRICSRRTSSGV
                     LLVISDLTEEYRLEQSRRDFIADAGHELQTPLASIRLAAEFLMEGPKRSEEDEKCLTT
                     IIAQQERMTRLVDDLLLLSRMESEPPLHAEEEVDLCRLLSAVVEENRRHPLAGRIGVE
                     EEYECAAPAVVRSEDISRALNNIVENAVKYVHEKFGDEEGGRILVSLRPEQLFWSIRV
                     EDNGPGISSETAGVLFQRFRRGDSHRARGKWGKGGYGLGLAIARKIVLDAGGDILLHP
                     SDAGAVFEVLLPRKD"
     gene            complement(2873..3562)
                     /locus_tag="PK849_02010"
     CDS             complement(2873..3562)
                     /locus_tag="PK849_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE64926.1"
                     /translation="MPEARILIVEDEDAIMEIVSQALRRHGYETASASDGDAALEKVF
                     SLRPDLVILDLMLPKMDGWEVCRRMRAEPETERIPILMLTARRDERDVVEGLELGADD
                     YMKKPFSLAELTARVKALLRRTGERRESRIVENGDLRIELDDETALLRGTPLDLSPTE
                     FRLLEALARKMGRTASREELLSKIWSFYGGDTRTVDVHISRLRKKLDDGKSPALAINS
                     LRGRGYRLVWE"
     gene            complement(3568..4290)
                     /gene="phoU"
                     /locus_tag="PK849_02015"
     CDS             complement(3568..4290)
                     /gene="phoU"
                     /locus_tag="PK849_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPE64927.1"
                     /translation="MEPVSTRKQLEADLTELKRMVLRLGKMAEESISKAIWALKNQDA
                     ELARMVLAKDDDIDDLAEALDNACMHFAARFQPLGEDLRAVTSIMHMAVDLERIGDYG
                     GNIAKVAAELADKELIKPLIDIPRMLDVLSAMMSKALAAFDTNNAEMAKEVFAMDDMM
                     DDLEKQIMRELLLLMMERPQRIEQATQLLNVARTLERGGDHVTNVAERVVYILTGKTV
                     KASAFRRPRPAALNGRKEHEER"
     gene            complement(4319..5077)
                     /gene="pstB"
                     /locus_tag="PK849_02020"
     CDS             complement(4319..5077)
                     /gene="pstB"
                     /locus_tag="PK849_02020"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPE64928.1"
                     /translation="MEKILVSSRGVNLYYDSARVLKDITFDVMEKQVTALIGPSGCGK
                     SSFLRCINRMNDFIPSARVEGEILVGGSNIYAGGVDVIALRRNVGMVFQKPNPFPMSI
                     YDNVAYGPRLHGVKQKDRLDETVKQSLVGAALWDEVKDKLKTPGTGLSGGQQQRLCIA
                     RAIATEPEILLMDEPTSALDPMATARIEELVRELKKRYTVVIVTHNMQQAARISDRTA
                     FFLMGDLVEYDATPKMFTSPEDKRTEDYITGRFG"
     gene            complement(5094..5948)
                     /gene="pstA"
                     /locus_tag="PK849_02025"
     CDS             complement(5094..5948)
                     /gene="pstA"
                     /locus_tag="PK849_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPE64929.1"
                     /translation="MDGRRFADMMMTVLSWAAALLLTGMVVAIIGFVVVRGYSVLSLE
                     FLIEPPRDSMTRGGIMTPLVGTLQLVGVAMFFAIPVGVATGLYFAEYARDDMFTSILR
                     LAIRSLAGVPSVVFGLFGLSLFVILLGFGPSLLAAALTLACLTLPLMVTASEQAFLAV
                     PKDYRDASYALGASKWQTIRKVVLPAAAPTVITGIILSVGRVAGETAPIMFTGAAFFA
                     PHIARSLFSEVMALPYHIYVLATAGTFIEETRPIQYGTVLVLLLLVLGICSGGIYMRS
                     RLRRLMRR"
     gene            complement(5949..6812)
                     /gene="pstC"
                     /locus_tag="PK849_02030"
     CDS             complement(5949..6812)
                     /gene="pstC"
                     /locus_tag="PK849_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPE64930.1"
                     /translation="MKDRLPGIVVKGMSSAGIFIMIFILYFLFREGIPVLKAVTLKDL
                     FFGDLWYPAENPPVLGMFPLIVGTLAVTAASSLLALPFSLLIAVFVSEVAPRPVRELL
                     KPVLELLGFFPSIVLGFIGMVVLAPWLQETFDMLSGLNLLNASVLLGVLTVPIVSSLA
                     EEALSAVPADLRDASYALGATRFETTRKVVVPSALPGILSASLLGVMRALGETMVVLM
                     AAGGAAIIPLSIFDPVRPLTSTIAAEMGETPVGSPHYAALFFGGIILLLMTLGINLLS
                     AWIEKKGKLVQ"
     gene            complement(6927..7745)
                     /locus_tag="PK849_02035"
     CDS             complement(6927..7745)
                     /locus_tag="PK849_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE64931.1"
                     /translation="MKKFAAVLAACAVLSISGAAFANAIVMNGSTTVLPIAQAAAEKY
                     MAANSDVKISVSGGGSGNGIKAIIDGTTDIADSSRFIKTDEVKAAVEKGTYPVPFAVA
                     MDALLPIVHPENPVSDLSIEQLRDIYAGKITNWKDVGGDDKGIAVVGRDTSSGTYETW
                     QEIIMKKERVSQRALVVASSGAMLTTVAKNKYAIGYDGIGYVNNTVKPLMVGGKKGSA
                     ETALDGTYPIARFLYMFTKGWPTGEIMKFINFVLSDEGQKIVAETGFVPLRVLK"
     gene            8208..8621
                     /locus_tag="PK849_02040"
     CDS             8208..8621
                     /locus_tag="PK849_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPE64932.1"
                     /translation="MKSERTTAIYPAIFSHAEDGITITFPDLPGCISCAQTDEEALHM
                     ARDALGAWIVATEDLGEPVPLPSPTSKLRPKKNEAVCLVDVWLPLFREEKRSGSVKKN
                     VTVPLWLNVLAEKAGLNFSQILQAGLKSTLGIQDR"
     gene            complement(8877..9098)
                     /locus_tag="PK849_02045"
     CDS             complement(8877..9098)
                     /locus_tag="PK849_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64933.1"
                     /translation="MPMTVEREKLAQNILALTDEEVSAVAAFVRNLREDEPPLSDDEL
                     AQMAESDEDIAAGRLTPWKEARQRLEALP"
BASE COUNT         1914 a         3106 c         2561 g         1678 t
ORIGIN      
        1 gagggaaccg aaaagtgaac aggatacttg cgatcgacga taaggccgat aatctcgtgg
       61 tgcttcgggc aatgatgaag atgctccttc ccgaatgcgt tctgctgcag gcgctctcgg
      121 gtgaagaggg actccgcatc gccaaggagc agcgccccga tacgattctg ctcgacattt
      181 tcatgccggg gatggacggc tatgaaacga cgatgcgcct caaggccgac cctgaaacgg
      241 catccattcc ggtgatcctc ctgaccgccg ccgaaaacac ggcggcgaac cgcaccgcgg
      301 gactgaaggt cggcgccgac gcgttcctct ccaagcccat cgaggaggag gaactggttg
      361 cgcaggtgcg cgcaatgctc cgcgtcaagg agtccgaaac ggcgctgaag acgaagatgg
      421 acgtactcga agatctggtc gaacagagaa ccaaagagct gaaggagagc gcgaaccgcc
      481 ttgccctgac cctcgaccag accatctccg tgctggcgaa caccgtggag acaaaagacc
      541 cgtacacggc gggacaccag gtccgctgcg cctccctcgc cctcgccctg gggaacgagc
      601 tgcggctcga cccccagcag cttcgcggca tccacatggc cgcaagaatc catgatatag
      661 ggaagatcaa gatcccctcc gagatcctca gcaaaccggg gaaaatcacc ccgctcgagt
      721 tctcgctcat aaaggaacat ccgggcgtcg gcgaggccat cgtcgcccct atcgtctttc
      781 cgtggcccgt cggccggatg atcgcacagc atcacgaacg aatcgacggc acggggtatc
      841 ccaaagggct cttcggcgac gacatcctga tggaggcgaa ggtcatcgcc gtcgccgacg
      901 tcgtggaggc catctcctcc taccgcccct accgcccggc gctcgggata gacgccgcgc
      961 tccaggagat tgaaaacggc atcggaacga aatacgatcc cgacgccgtc cgcgcatgca
     1021 ctgccctctt cagggaaaac cgattcgaat tcgacgaatg aaaaagggcg gaagagcccg
     1081 tttcggagct cttccgccct gcgttcctct ttcgccgcgg atcagtcctt ccggggcagc
     1141 agcacctcga agacggcgcc cgcatcggac gggtgaagca gtatatcccc tcccgcgtcg
     1201 agcacgatct tcctcgcaat cgccaggccg agcccgtagc cgcccttccc ccacttcccg
     1261 cgcgcccggt ggctgtcgcc cctgcggaaa cgctggaaaa gcacgcccgc agtctcggac
     1321 gaaatcccag gcccgttatc ctccacacgg atactccaga aaagctgctc cggccgaagc
     1381 gacaccaaaa ttctgccgcc ctcttcgtcg ccgaacttct catggacata cttcaccgcg
     1441 ttctcgacga tattgttcag cgcgcgggag atatcctcgc tccgcaccac ggcgggggcc
     1501 gcgcattcat actcttcctc gaccccgatg cgccccgcga gaggatgtct ccggttctcc
     1561 tcgaccacgg cggacagcag cctgcacagg tcgacctcct cctccgcgtg cagcggcggc
     1621 tccgactcca tccgcgaaag cagcagcaga tcgtcgacga gccgggtcat ccgctcctgc
     1681 tgcgcgatga tggtcgtcag acacttttcg tcctcttcgc tccgcttcgg cccctccatc
     1741 aaaaactccg cggccagcct gatgctcgcc agcggcgtct gaagctcatg ccccgcgtcg
     1801 gcgatgaagt cgcgcctcga ctgctccagc cggtactcct ccgtgaggtc ggagatcacg
     1861 agcagcaccc cgctcgacgt ccgtcgcgag cagatacgaa gatagcgctt gtccagctcc
     1921 ggaagatcca gcgtcaggca gtcctcttct ccgttcatgg ccgcctccac aagggggtac
     1981 atatccccgg aaggaagaat ccgctccacg gggacgccgt gcggcgcctc ctccttcacg
     2041 cagagaagcc gcttccccac gtcgttcagg taccggaccc tccgctcttc gtcaagcaga
     2101 accacgccca caggcagcgc cgtcacgatg cgcgaaagat cctcgcgttc cgtgcgaagc
     2161 tcggccagcg ccccacgaag ccgggcggac atcccgtcga gcgcgctgga aagccgttgc
     2221 agctccggct ccgccatgat cgggaagcgg accgcctccc cctgcgcaat cttgcccgcg
     2281 aactccacga tccggtcgag cggacggaaa aagagcctcg ccagccacgt gccgaacgcg
     2341 acgatcagca ccacggcgac gaaaagatag aagatgaact tgttcctggt ctccgcgaag
     2401 gcctccgaca aaaactccag cgggtacgcc gccctgagta ccgcgacgcg cccctcggcc
     2461 tccaccttgc gggcgaagta gaggtagtac atgttcgtcg tcgcgctgta gcgcagcgcg
     2521 gacccctcgc ccgaagcgag cgcccccttc acctccggcc tggtcaggtg gttttccatc
     2581 gcctccgggc tccgctccgt gtcggcaagc accacgccgt tggcgccgat cagcgtgacc
     2641 cgtccgcgga gaatatcctc ccatttttcc acattcgcgg taaatcgctc cataccgccg
     2701 tccttcagcg acgccgcaag aagcgccgtc tgctgcgaaa gctccagcgt gctctgtcgg
     2761 acgatgccgt tccgcaccac cccgtacagc agcagccagc tgataccgaa cgcgagcatc
     2821 accgccagcg agatcaccgc gacaatcttt ttcttcaacg tgaacatgga catcactccc
     2881 acaccagacg ataccctctc cctcgaaggg agttgatagc caacgccgga gactttccgt
     2941 cgtcaagctt cttccgcagg cgagagatat gcacgtccac ggtccgcgtg tcgccgccgt
     3001 agaagctcca tatcttcgac agcagctcct cgcgcgaggc agtccgcccc atcttccgcg
     3061 ccagcgcttc caggagccgg aactccgtcg ggctcagatc gagcggcgtc ccgcgcagca
     3121 gcgcagtctc gtcgtcgagc tcgatgcgaa gatcgccgtt ttcaacaata cggctctccc
     3181 gccgctcccc ggtgcgccgc agcagcgcct tcacgcgcgc cgtcagctcc gcgagcgaga
     3241 acggcttttt catgtagtcg tcggccccca gctccagccc ctccaccacg tcccgctcgt
     3301 cgcggcgcgc ggtgagcatc aggatgggaa tccgctccgt ctccggctcc gctctcatcc
     3361 ggcggcagac ctcccagcca tccatcttcg gcagcatcag gtcgaggatc accaggtcgg
     3421 gtcggagaga gaagactttc tccagcgccg cgtcgccgtc agaggccgag gccgtctcat
     3481 agccgtgccg ccgcagcgcc tgggagacga tctccatgat cgcgtcctcg tcctccacaa
     3541 tgagaatccg cgcttccggc acgtccctca ccgctcctcg tgttcttttc gtccgttcag
     3601 agccgcaggg cgcggacgcc ggaacgccga agccttcacg gtcttcccgg tgagaatgta
     3661 gaccacccgt tcggccacgt tcgtcacgtg atcgccgccc ctctccagcg tccgcgccac
     3721 gttgagcagc tgcgtcgcct gctcgatgcg ctgcggccgc tccatcatca ggagcagcag
     3781 ttccctcata atctgtttct ccagatcatc catcatgtcg tccatggcga agacctcctt
     3841 cgccatctcc gcgttgttcg tgtcgaacgc cgccagagcc ttcgacatca tcgcggaaag
     3901 gacgtcgagc atccgcggaa tgtcgatcag gggcttgatc agctccttgt ccgcaagctc
     3961 cgccgccacc ttcgcgatgt tgccgccgta atcgccgatg cgctccaggt ccaccgccat
     4021 gtgcatgatg gaggtcaccg cccgcagatc ctcgcccaaa ggctggaagc gcgcggcgaa
     4081 gtgcatgcag gcgttgtcca gcgcttcggc gaggtcgtcg atgtcgtcgt ccttcgccag
     4141 caccattctg gcaagctccg catcctgatt cttcagcgcc caaatcgcct tgctgatgga
     4201 ctcctcggcc atcttgccga ggcggagcac catccgcttc agctccgtca aatccgcctc
     4261 aagctgtttc cgtgtactca caggctccat gctcatcgtc tcctctctcc cggccttcct
     4321 agccgaaccg ccccgtgatg tagtcctccg tccgcttgtc ctccggcgac gtgaacatct
     4381 tgggcgtcgc gtcgtactca acgaggtcgc ccatcaggaa gaaggcggta cggtccgaga
     4441 tgcgggcggc ctgctgcatg ttgtgggtta cgatcaccac agtgtaccgc tttttcagct
     4501 cgcggaccag ctcttcgatg cgagccgtcg ccatcgggtc gagcgcgctc gtcggttcgt
     4561 ccatcagcag aatctccggc tccgtcgcga tcgcccgcgc gatgcacaaa cgctgctgct
     4621 gcccgccgga gagccccgtg ccgggagtct tcagcttatc cttcacctcg tcccagagcg
     4681 ccgcgcccac aaggctctgc ttcacggtct cgtccagcct gtccttctgc ttgacgccgt
     4741 gcaggcgagg cccgtacgcc acgttgtcgt agatcgacat cgggaagggg ttcggcttct
     4801 ggaagaccat ccccacgttc cggcggagcg cgatcacgtc cacgccgccc gcgtagatgt
     4861 tggaaccgcc gaccagaatc tccccctcaa cccgcgcgct cgggatgaag tcgttcatcc
     4921 ggttgatgca gcgcaaaaag ctgctcttgc cgcaccccga cggcccgatg agcgccgtga
     4981 cctgcttttc cataacatca aacgtgatat ccttcagcac gcgcgccgaa tcgtaataca
     5041 gattcacgcc gcgtgaactc accagtattt tttccattat agtccctcca cccctatcgc
     5101 cgcatcagcc tgcggagacg ggaacgcatg tagatgcctc cggaacagat gcccagaacc
     5161 agcagcagca gcacgagcac tgtaccgtac tgaatcggcc gggtttcctc gatgaacgtc
     5221 cccgccgtgg caaggacgta gatgtggtac ggcagcgcca tcacctcgga gaagaggctg
     5281 cgcgcgatgt gcggcgcgaa gaaggccgcc cccgtgaaca tgatgggcgc cgtctccccc
     5341 gccactctgc cgacgctcag aatgatcccc gtgatcaccg tcggcgccgc ggcgggaagc
     5401 accaccttgc ggatggtctg ccacttcgag gccccaagag cgtacgaggc gtcacgatag
     5461 tccttcggca ccgccagaaa cgcctgctcc gacgccgtca ccatcagcgg cagcgtcaga
     5521 caggcgaggg tgagcgccgc ggccagcagg ctcggaccga accccagcag aatcacgaag
     5581 agcgacaggc cgaagagccc gaacaccacc gacggcacgc ccgcaagaga gcgaatcgcc
     5641 agccgcagaa tcgacgtgaa catatcgtcg cgagcgtact ccgcgaaata cagcccggtc
     5701 gccaccccca cgggaatcgc gaaaaacatc gccacgccca ccagctgcaa cgtccccacc
     5761 aggggcgtca tgatcccccc tcgcgtcatg ctgtcgcggg gaggctcgat cagaaattcc
     5821 agcgagagca cgctgtaccc ccgcaccacg acgaagccga tgatcgcgac caccattccg
     5881 gtcagcagca gcgccgccgc ccaggagagg accgtcatca tcatgtcggc aaatctgcgc
     5941 ccgtccatct actgcaccaa ctttcccttc ttctctatcc acgcggagag aagattgatc
     6001 ccgagcgtca tcagcagcag gatgataccg ccgaagaaga gcgcggcata gtgcggcgac
     6061 cctaccggag tctcccccat ctccgccgcg atggtggagg tcagcggacg caccgggtca
     6121 aaaatcgaga gcgggatgat cgccgcgccg cccgccgcca tcagcaccac catcgtctcc
     6181 cccagcgcgc gcatcacccc aagcagcgac gcgctcaaaa taccggggag cgccgagggc
     6241 acgaccacct tccgcgtcgt ctcgaaccgg gtcgcgccga gcgcgtagga ggcgtcccgc
     6301 agatcggcgg gcaccgcgct cagcgcttcc tcggcgagcg aactcacgat cggcacggtc
     6361 agcaccccga gcaataccga ggcgttcagc agattcagcc ccgagagcat gtcgaacgtc
     6421 tcttgcagcc agggcgccag caccaccatc ccgatgaacc ccagcacaat ggaggggaag
     6481 aagcccagca gctcaagcac cggcttcagc agctcgcgca ccggcctggg cgccacctcg
     6541 gagacgaaaa ccgcgatcag cagactgaac ggaagcgcca gcagcgagga ggccgccgtc
     6601 accgcaagcg ttcccacgat aagaggaaac atccccagca ccggaggatt ctccgccgga
     6661 taccacagat cgccgaaaaa cagatccttc agcgtcaccg ctttcagcac gggaataccc
     6721 tctctgaaca ggaaatagag gatgaagatc atgatgaaga tccccgcgct cgacattccc
     6781 ttgactacaa ttcccggaag cctgtctttc atgaaaaaac ctctttcgtt cgtcgaattc
     6841 gtcgccctcg gcgggcccgg aaaaagcccc ctccccgtga ggggaagggg tacggctgga
     6901 ggaactctct ggatgagtcg tcgatgttat ttcagtactc tcaggggaac gaatccggtt
     6961 tcggccacta tcttctgtcc ctcgtcgctc aggacgaagt tgatgaactt catgatttcg
     7021 ccggtgggcc agcccttcgt gaacatgtag aggaagcgtg cgatggggta cgtgccgtca
     7081 agcgccgtct ccgccgagcc tttcttgccg cccaccatca ggggcttcac ggtgttgttg
     7141 acgtagccga tgccgtcgta gcctatcgcg tacttgttct tggccacggt ggtgagcatg
     7201 gctccggagg aggcaaccac aagcgcgcgc tgggaaacac gttccttctt catgatgatc
     7261 tcctgccacg tctcgtaggt gccggaactg gtgtcgcggc ccacgaccgc gattcccttg
     7321 tcgtcgccgc cgacgtcttt ccagttggtg atcttgccgg cgtagatgtc gcgaagctgc
     7381 tcgatgctca ggtcgctcac ggggttctcc gggtgcacga tgggaagcag cgcgtccatt
     7441 gccaccgcga agggcaccgg gtacgttccc ttctccaccg cagccttcac ctcgtccgtc
     7501 ttgatgaagc gggaggagtc ggcgatgtcc gtggtgccgt cgatgatcgc cttgatgccg
     7561 ttgccgctgc cgccgccgga aacgctgatc ttgacgtcgc tgttggccgc catgtacttc
     7621 tctgccgccg cctgggcgat gggaagcacc gtggtggaac cgttcatcac aatggcgttc
     7681 gcaaacgccg cgccggaaat ggagaggacc gcacatgccg ccagaactgc cgcgaatttc
     7741 ttcataccga atagcctcct tgatttctca tcttggtgtg ccggggacca tccccgatcg
     7801 acacgcacag tataggaggc gagcgtaacg gatgttacaa aagtatgtaa cagatgagta
     7861 acgatgacgt tacgttcgta acggggggaa gaagcatcat tttatacgca taaaatacaa
     7921 gtacgtatat atcatacttg acgtctcact aaaggagtat aaaatatact tatcattcaa
     7981 agggggggtg ctggtaatcg gtagggatga aatagtcaaa attctactgg aagccggttg
     8041 gatcagcaca ggaagaggca aaggaagcca catggttttc tttcatccgg agacgaagca
     8101 gagaaccacg attccatacg gcaaggatat tcccaaagat actcttgctg ctatacgacg
     8161 tcaaacaggg atcaaagaaa tacgctaaaa aagaaggagg cgaaataatg aaaagcgaac
     8221 gcacaaccgc aatctatccg gcgatcttct cccatgcgga agacggtatc acaataacct
     8281 tccccgacct cccgggatgc atttcctgcg ctcaaacaga cgaggaggcg ctgcatatgg
     8341 cacgagacgc cctcggagca tggatcgtcg ccaccgagga cttgggagaa ccagtcccgc
     8401 tgccctctcc tacttcgaaa ctacgcccga aaaagaacga agccgtctgc ctggtcgacg
     8461 tgtggctgcc gctcttccgc gaagaaaagc ggtccggctc cgtaaagaaa aatgtcacgg
     8521 tcccgctttg gctgaacgtg ctggctgaaa aagccgggct gaacttctcc caaattctcc
     8581 aggcagggct taaatccact ctcggaattc aagaccgttg aggagctgtg gaaaatgacg
     8641 gaccacaaga ttgaagacat gtggatcaaa gaagcctccc gccgggaacg tgagatggat
     8701 acgggcatgg cacgttgcat acctgcggat gaggcgctgg aaagagctta tcaacggcgc
     8761 aggagcagag agtagctgtt tttttaaaaa acacccggga aaatatggag gctttgcttt
     8821 tatttttcat caccatgctg attcaagaag atcacgaaaa gagtgtgtta ccaacgtcac
     8881 ggcaacgcct caagacgttg tcttgcctct ttccaagggg tcagacggcc cgccgcgatg
     8941 tcttcgtcgg actcggccat ctgtgccagt tcgtcgtcgc tcaatggcgg ctcgtcctca
     9001 cgcagattgc gcacaaacgc agccaccgca gaaacttcct cgtccgtaag tgccagaatg
     9061 ttttgcgcca gcttttcccg ttccacggtc atcggcatag tcgtcacccc cctttcatcg
     9121 tcattatagc gtgcttcata gcgcaccgca gagagagcgt ccattaaagc atgatgcctc
     9181 tcaggaaaca ggctcggcct tcttcccgga ctcctgttca agaaagaccg atggctgtat
     9241 gctattatga gcgttggga
//
LOCUS       DAOPKU010000037         9119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8024_length_9119_cov_5.156774, whole genome shotgun sequence.
ACCESSION   DAOPKU010000037 DAOPKU010000000
VERSION     DAOPKU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9119
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8024_length_9119_cov_5.156774"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PK849_02050"
     CDS             complement(<1..249)
                     /locus_tag="PK849_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE64934.1"
                     /translation="MHKIHLDDVEGTLFPAGRRTKVLVGKDSPLQAKGFVMGRVRVFP
                     GGSIPAHEHFNEEIYHILSGTGEMQVGGETGEVRAGDTV"
     gene            394..1137
                     /locus_tag="PK849_02055"
     CDS             394..1137
                     /locus_tag="PK849_02055"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPE64935.1"
                     /translation="MNESRKILVEKVYAAIKDGSIVKDGKLPTEREMAALMTTSRTSM
                     REALIVLETLGIIEVKGKQGLFVKDAGMGRLNQSLDLYATWPADIIPQIFQVRIMMES
                     PAAGLAARNRTDADLAKMEECLAQFSRIFGEDGPDAGREGAHWNDIFHRIVIAASHNE
                     VLIRMHEGLSSIIERAMESLNKNRLTTPRPQWTERILGEHEQIVRAIRERDETGARES
                     MRKHLEISAANLEKLCQDKESALLGLPVG"
     gene            1334..2689
                     /locus_tag="PK849_02060"
     CDS             1334..2689
                     /locus_tag="PK849_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64936.1"
                     /translation="MAQYEPFIYETLDELRRKIQSLGLDISFSEDLSVLAAPAAVGGR
                     TAPNRFSTLPMEGRDSLPDGSPGELTFRRYRRYGAGGWGLIQFEACAVWQGGRSSDRQ
                     MCIQPSAEKAIASLLEAARAAAREPFGPSHSPLCLLQLQDAGRYRHATGTTPGLTVRY
                     PLLDRRAKVPEHVEPLSDADIEAVQDRMVEAAVRAEKLGFDGIDIKSCHRYLASELLS
                     AHLRPGRFGGGFDGRTRFLRETTERIRSAVSNPSFLVTSRMNLFDGFPYPYGWGCDRS
                     DPPVPDMSEPLQLVGILKGLGLNLLTTSTGTPYYNPWLVRPFDRLIPGNIEAPEHPLE
                     GVARLFALTARVRKEYPDLPTAGSGYTWLRQFGVFAAAANVARKAVTFAGFGRTGFAY
                     PELPRDVLEKGAVDPKKVCVSCSLCSEVMGQGGSTGCYAKDREVYGPMLKAHRASRKE
                     H"
     gene            2720..3880
                     /locus_tag="PK849_02065"
     CDS             2720..3880
                     /locus_tag="PK849_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE64937.1"
                     /translation="MKNGTRKWLFCAVLVVLLAGLTGMAVAKELLIGNMQDISGPTSV
                     WGNAVTRGAEIAIEKINAAGGIDGAMLKLVTMDTKANVQEAIKGYNSLVSSGVVAVVG
                     PPVSNIGLALGPIANKAGVPVVGSFIDPRVTVGEDGKPHAAMFLMQPSSIQYSEIMAS
                     YTIDVLGLKKVGVLYDQSNAFAVSLMKPYVDYIKAAGGEIVSEQVYTKGDKDFKTQLL
                     KIKDAGADCLYFPNYIQDAVLAMNQRKQVGLDVPVIGGLDFAPPFASLVNDPEATNDI
                     YLANNFSETEPQLKEVWEIYKTKYGEDPVNKVFLGYDKVLLIREAMKLGGGTSAAQVM
                     AGLSQVKDLQGTTGVLTISPGTHQPVGLSMVMYKIEKGKYVELGRYVPEKHK"
     gene            3990..4880
                     /locus_tag="PK849_02070"
     CDS             3990..4880
                     /locus_tag="PK849_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE64938.1"
                     /translation="MLVQNILNGISLGSVYALIAVGFALIFNVLKFSNFSQGGVMTVT
                     AYAGYVASRYLSDRLDSLALFAAVLAAAALSGAVIAAAVERLAFRRLRRTKSEPVYYF
                     ISSITMGILLENLITIYASSNFYSYPRFFTTSAVPLFGVNVAVTDMMMFALSITSLLA
                     LLFILHRTKIGLGIRAACFDVNTVQLMGMNPDFLVTTALMISGALGGVSGVFLGMNYT
                     LYPQLGQMVVKGFVASVLGGLGSIHGAVIGAVLLGVLEIFFISWVGAGWAPVFVFIVM
                     LAFLLVRPRGIAGIIVQEKA"
     gene            4891..5754
                     /locus_tag="PK849_02075"
     CDS             4891..5754
                     /locus_tag="PK849_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE64939.1"
                     /translation="MDYFVSTVIFTGIALIGVLGVYLITGLTGLFSLGQAAFMAIGGY
                     VSAVLIRNYSVPVVPAVALAIAAAMLAALLVGLPTVRLRRDYISLVTLGFGEAIVALL
                     NNSANITGGAMGISGIPQLTTLPVVLVSVVVCLGLVILYKNSKYGRQCLALRSDELAA
                     KSMGINVYRLKLITFLFAGALTGYSGVLFGFYTTYLEPAAFNWIISAEWTIIVFVGGV
                     NSLTGTVLATILLTGLPEFLRFASEWRVAIYCVIVLLIVNYRTAGIMGEHELSPKTLR
                     RLFGKTKGAPR"
     gene            5751..6530
                     /locus_tag="PK849_02080"
     CDS             5751..6530
                     /locus_tag="PK849_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64940.1"
                     /translation="MSLLEVRNLTKSFGGVRAVDDFSFGAEQGEIVGIIGPNGAGKTT
                     AFNLVTGVYPLDRGSILLGGKELAGKEQFEITRSGIGRTFQNIRLFKGLSVLENVMTA
                     ADPYSQYGLFSLFTLGAAFRRREREVRAAAGEYLSLVGLEKYADEKPSNLPYGLQRKL
                     EIARALATEPKVLLLDEPAAGLNPSEVREFIGLVGELRERFGFAVLVIEHRMEVIMTL
                     CSRIYVLNFGKLLATGTPAEVRANREVTEAYIGKEDGSCSV"
     gene            6518..7252
                     /locus_tag="PK849_02085"
     CDS             6518..7252
                     /locus_tag="PK849_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64941.1"
                     /translation="MLRIEDLWVSYGHVQALLGVSLEVKRGSIVSIIGSNGAGKSTLL
                     NTISGLVKQERGKIFLTEGIGPDTAKLLPREPYRVVKLGVVQVPEGRQVFPGLTVREN
                     LMAGAYTVNDAGRVRRNLDRMFEMYPILKERARQQAGTLSGGEQQMLAICRGLMAEPK
                     VLLLDEPSLGLAPIIVKGVFDTVRRIRDEGITVVLVEQNANQALAIADYAYVLENGRV
                     ALEGEGRALLCDPAVKDAYLGGAQEA"
     gene            7291..8625
                     /locus_tag="PK849_02090"
     CDS             7291..8625
                     /locus_tag="PK849_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64942.1"
                     /translation="MVNKLFVQPLLFASPVYWPDRAELDAAGKAVAGMLEAMDAPTSL
                     MRTARIVAGDTDLDDALSSLGRAVPLLVPLSGGVQKRMLAAAGAASESLLWSYFPNDD
                     IYPEEERPTITRIVSKNALPAVADVWGTLRNRGCRVAKVYHEGSWLAAARELKAAHRV
                     RSARLLLVGYTQEWVVSASVDTRKMERLLGISSVHIGLEELFAEFEACGNDSVAASLA
                     KEFADGARSCLEPNMEQIEQAFRMYRALKNLLARYGCNALAISCFSLAKTLGVTACVA
                     LSMLNDEPETVAACEGDLDSAVSMIVGKAITGMPVFMGNPVYNLENTLDIVHCTAPRK
                     LLSANAQPYEIRSHHETGLSVSQRVEVEGERDATIFRIGNEFRNAVLFEAPLLSNPRL
                     DTCRTQFRFGLKSWEACFDAMLGCHHMVAFGRHGDALERFMKQRLGLEVVRF"
     gene            complement(8788..>9119)
                     /locus_tag="PK849_02095"
     CDS             complement(8788..>9119)
                     /locus_tag="PK849_02095"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPE64943.1"
                     /translation="PVEIICYHSQQSAEKYIKALMVSSGEEPPKTHNLIDLARICKEK
                     KIAAEIWDGMASEIVGLNRFGTVPRYPSKIELAESDARRAVEDASSIREVITGRIPSQ
                     TSCPGPI"
BASE COUNT         1602 a         2871 c         2973 g         1673 t
ORIGIN      
        1 gaccgtgtcg ccggcgcgga cctcgcccgt ttcgccgccc acctgcattt cgcctgttcc
       61 ggagaggatg tggtagatct cctcgttgaa gtgttcgtgc gcggggatgc ttccgcccgg
      121 aaagacgcgg acgcgcccca tcacgaaccc cttcgcctga agcgggctgt ccttcccgac
      181 gagcactttc gtcctgcgcc ctgcaggaaa gagcgttccc tccacatcgt caagatgaat
      241 cttgtgcatt cgggcatcgc ctcctcgact gaagcgtttt tcgtgtataa tgcggttgtc
      301 catggacaac cataagtgga accatactcc ccttggcgcg tctctataat agcatctcgg
      361 ccgaaagacg gggagtcgaa accggaggaa gaaatgaacg aatcgagaaa aattctggtg
      421 gagaaggtgt acgccgcgat taaggacggc tccatcgtaa aggacggcaa gctccccacg
      481 gagcgcgaga tggccgcgct gatgaccacc agcaggacgt cgatgcgcga ggcgcttatc
      541 gtgctggaga cgctcgggat catcgaggtg aaggggaagc aggggctctt cgtgaaggac
      601 gcgggcatgg ggcggctcaa ccagagtctc gacctgtacg cgacgtggcc ggcggatatc
      661 atcccccaga tcttccaggt gcgcattatg atggagagtc cggccgcggg gctcgcggcg
      721 cgcaaccgga cggacgcgga cctggcgaag atggaggagt gcctcgcgca gttctcgcga
      781 atcttcggcg aggacgggcc cgacgcgggg cgcgaggggg cccactggaa cgacatcttc
      841 caccgcatcg tcatcgcggc ctcccacaac gaagtgctca tccggatgca cgaggggctc
      901 tcctcgatca tcgagcgcgc gatggagtcg ctgaacaaga accgcctcac cacgccgcgt
      961 ccgcagtgga cggagcgcat tctgggcgag cacgagcaga tcgtccgggc gatccgcgag
     1021 cgggacgaga cgggcgcgcg cgagtcaatg cggaagcatc tggagatctc cgccgcaaac
     1081 ctggaaaagc tctgtcagga caaggagtcg gcgcttttag gactacccgt cggatagtaa
     1141 cgaacagcgc tcttttctcc cggttcgccg gttccggcgg aggctttccg gcctgagggt
     1201 atttgaaacg ctccggggct tctcaatctg aggagcaaat ggtataattg cgtcagtttt
     1261 ggttgtcctt ggtcaaccca agaattgaat ccggcgcctt cccacggaag gcccgtcaca
     1321 gagagggagg cccatggcac agtacgaacc attcatctac gaaacactgg acgagcttcg
     1381 gcggaagatc caatcgctcg gcctcgacat ttccttcagc gaggatctct ccgttctcgc
     1441 cgcgcccgcg gcggtcggag ggaggaccgc tcccaaccgc ttctcgacgc ttcccatgga
     1501 ggggcgcgac tcgctccccg acggttctcc gggcgaactc acgttccggc gctaccgccg
     1561 ctacggcgcg gggggctggg ggctgatcca gttcgaggcg tgcgccgtct ggcagggggg
     1621 gcgcagcagc gaccgtcaga tgtgcatcca gccctccgcc gaaaaggcga tcgcctcgct
     1681 gcttgaagcg gcgcgcgccg cggcccgcga acccttcggt ccgtcgcatt cgcctctctg
     1741 cctgcttcag cttcaggatg ccgggcgcta ccggcacgcg acggggacga cgcccggcct
     1801 gacggtgcgc tatccgctgc tcgacaggcg cgcgaaggtt cccgaacatg tggagcccct
     1861 ttccgacgcc gacatcgagg cggtgcagga tcgcatggtc gaggccgccg tccgcgcgga
     1921 gaagctcggc ttcgacggca tcgacatcaa gagctgccac cgctacctcg cctcggaact
     1981 gctttcggcc catctacgcc ccggaaggtt cggcggcggt ttcgacggca ggacgcgctt
     2041 cctgcgcgag acgacggagc gcatccgctc ggctgtctcg aatccttcgt tcctcgtgac
     2101 cagccggatg aatctcttcg atggctttcc gtatccgtac ggctgggggt gcgaccgctc
     2161 cgacccgccc gttcccgaca tgagcgagcc gctgcagctc gtcggcatcc tcaaagggct
     2221 cggactgaac cttctgacca cctccaccgg tacgccctat tacaatccgt ggcttgtgcg
     2281 cccgttcgac cggttgattc cggggaacat cgaggcgccg gaacatccgc tcgaaggcgt
     2341 cgcgcgcctc tttgcgctga cggcaagggt gcggaaagag tacccggatc ttccgacggc
     2401 cggttcgggg tacacgtggc ttcgacagtt cggcgtgttc gccgccgccg ccaacgtggc
     2461 gcggaaggct gtgacgtttg cgggctttgg acggacgggg tttgcgtacc ccgagttgcc
     2521 gagagacgtt ctggagaagg gggcggtcga tccgaagaag gtgtgcgttt cgtgcagttt
     2581 gtgcagcgag gtcatgggtc agggaggttc caccgggtgc tacgcgaaag accgggaggt
     2641 ctacggcccc atgctcaaag cgcatagggc gtcccggaaa gagcactaaa tacatttgaa
     2701 agcgggaggg aaaaaaagca tgaagaacgg tacgagaaag tggttgttct gcgcggtgtt
     2761 ggttgtactg cttgccggtc tgaccggtat ggcggtggcg aaggagcttc tgatcgggaa
     2821 tatgcaggac atcagcggcc ccacgtcggt gtggggaaac gcggtgacgc ggggcgcgga
     2881 gatcgccatc gagaagatca acgcggcggg cggcatcgac ggcgcgatgc tgaagctggt
     2941 cacgatggac acgaaggcca acgtccagga ggccatcaag gggtataaca gcctcgtgag
     3001 ttcgggcgtg gtcgccgtgg tcggacctcc cgtcagcaat atcggactgg cgctcggccc
     3061 catcgccaac aaggcgggcg ttcccgtggt cggcagcttc atcgacccgc gcgtcaccgt
     3121 gggcgaggac ggcaagccgc atgccgcgat gttcctgatg cagccctcca gcatccagta
     3181 ctccgagatc atggccagct atacgatcga cgtgctcggc ctgaagaagg tcggcgtgct
     3241 ctacgaccag tcgaacgcct tcgcggtctc cctgatgaag ccgtacgtcg actacatcaa
     3301 ggccgcaggc ggcgagatcg tctcggagca ggtgtacacg aagggcgaca aggacttcaa
     3361 gacgcagctg ctgaagatca aggacgcagg cgcggactgc ctgtacttcc ccaactacat
     3421 tcaggacgcc gtgctggcga tgaaccagcg caagcaggtc ggcctcgacg ttcccgtgat
     3481 cggcggactg gacttcgcgc cgccgttcgc ctcgctcgtc aacgaccccg aggcgaccaa
     3541 cgacatctac ctcgccaaca acttctccga aacggagccc cagctcaagg aagtctggga
     3601 gatctacaag acgaagtacg gcgaggatcc cgtgaacaag gtcttccttg gctacgacaa
     3661 ggtcctcctc atccgggagg ccatgaagct cggcggcggg acctccgcgg cgcaggtaat
     3721 ggcggggctg tcgcaggtga aggaccttca gggaaccacg ggcgtcctca ccatctctcc
     3781 ggggacgcac cagcccgtcg gactctccat ggtcatgtac aagatcgaaa agggcaagta
     3841 cgtcgaactg ggccgctacg tccccgagaa gcataagtag gcgacctgat actataattt
     3901 agaggccgcc ctttctcttt cgaaaagcgg ggcggcctct catttcttcc catccttccg
     3961 ccgccgggag gggcaaagga gctggtttca tgctggtgca gaacatcctc aacggtatct
     4021 ccctcggatc cgtctacgcg ctcatcgcgg tggggttcgc gctcatcttc aacgtcctca
     4081 agttctcgaa cttctcgcag ggcggcgtga tgaccgtgac ggcgtacgcg gggtacgtcg
     4141 cctcccgcta tctctcggac cggctggatt cgcttgccct gttcgcggcg gtgctcgcgg
     4201 cggccgcgct ttcgggggcg gttatcgccg cggccgtcga gcgtctggcg ttccggcggc
     4261 ttcgccggac gaagagcgag ccggtgtact atttcatctc gtcgatcacg atgggcatcc
     4321 tgctggagaa cctgatcacg atctacgcca gcagcaactt ctactcctac ccccgtttct
     4381 tcaccacctc ggccgtgccg ctgttcggcg tgaacgtcgc cgtgacggac atgatgatgt
     4441 tcgccctctc catcacctcg ctgctcgcgc tgctcttcat cctccaccgg acgaagatcg
     4501 ggctgggcat ccgcgccgcc tgtttcgacg tgaacaccgt gcagctcatg gggatgaacc
     4561 cggacttcct ggtgaccacc gcgctgatga tctcgggcgc gctggggggc gtcagcggcg
     4621 tcttcctggg gatgaactat acgctctatc cgcagctcgg gcagatggtg gtgaaggggt
     4681 tcgtcgcctc ggtcctcggc gggctgggga gcatccacgg cgcggtgatc ggcgcggtgc
     4741 tgctgggcgt cctcgaaatt ttcttcatct cgtgggtcgg ggccggatgg gcgccggtct
     4801 tcgtcttcat cgtgatgctc gccttcctcc tcgtgcgccc caggggaatc gcgggaatca
     4861 tcgtgcagga aaaggcgtag ggggctcgcg atggactact tcgtctccac cgtcatcttc
     4921 accggaatcg cgctcatcgg ggtgctcggc gtctacctca tcacggggct cacggggctt
     4981 ttctcgctgg ggcaggccgc gttcatggcc atcggggggt acgtctccgc ggtgctcatc
     5041 cgaaattact ccgtacccgt cgttcccgcc gtcgctctcg ccatcgccgc cgccatgctc
     5101 gccgcgctgc tcgtcgggct gcccacggtg cgcctcagga gggactacat ctccctcgtg
     5161 acgctcggct tcggcgaggc catcgtcgcg ctgctcaaca actcggcgaa catcaccgga
     5221 ggcgccatgg ggatctccgg catcccgcag ctcacgacgc tccccgtggt gctcgtcagc
     5281 gtcgtcgtct gcctcggcct cgtcatcctc tacaagaact cgaagtacgg acggcagtgc
     5341 ctcgcgctcc gctcggacga gctggcggca aagtcgatgg ggatcaacgt ctaccgcctg
     5401 aagctgatca ccttcctctt cgcgggcgcg ctcaccgggt attcgggcgt gctcttcggc
     5461 ttctacacga cctatctgga acccgccgcg ttcaactgga tcatctcggc ggagtggacg
     5521 atcatcgtct tcgtcggggg cgtgaacagc ctcaccggga cggtgctggc cactattctg
     5581 ctcactgggc tgccggaatt tctccgcttc gcctcggagt ggcgcgtggc catctactgc
     5641 gtgatcgtcc tcctgatcgt gaactaccga acggcgggca tcatgggcga gcacgaactc
     5701 tcgccgaaga cgctgcgacg cctcttcgga aagacgaagg gggcgccgcg atgagcctcc
     5761 tcgaagtgcg gaacctcacc aagtcgttcg gcggcgtccg cgcggtggac gacttctcct
     5821 tcggcgcgga gcagggggag atcgtgggga tcatcggccc caacggcgcg gggaagacga
     5881 ccgcgttcaa cctggtcacc ggcgtctacc ctctcgacag ggggagcatt ctcctcggcg
     5941 ggaaggagct ggcggggaag gagcagttcg agatcacgcg aagcggcatc gggcgcacct
     6001 tccagaacat acgtctcttc aaggggctct cggtgctgga gaacgtgatg accgcagccg
     6061 acccgtattc gcagtacgga ctgttctcgc tgttcaccct cggcgcggcc ttccggagac
     6121 gcgaacgcga ggttcgggcg gcggccgggg agtacctctc gctcgtcggg cttgagaagt
     6181 acgccgacga gaagccgtcg aatcttccgt acggccttca gcggaagctg gagatcgcgc
     6241 gcgcgctggc gacggagccg aaggtgctgc tgctcgacga acccgccgcg gggctcaacc
     6301 cgagcgaggt acgcgagttc atcggacttg tcggcgagct gcgggagcgc ttcggcttcg
     6361 ccgtgctcgt gattgagcac cggatggagg tcatcatgac gctctgttcg cgcatctacg
     6421 tgctcaactt cggcaagctg ctggctacag ggacgcccgc cgaagtccgc gccaaccgcg
     6481 aagtgacgga agcctacatc ggcaaggagg atggatcatg ctccgtatag aggatctgtg
     6541 ggtctcctac ggccacgtgc aggcgcttct gggagtctcg ctcgaagtca agcgggggag
     6601 catcgtcagc atcatcggct ccaacggcgc ggggaagagc acgctgctga acacgatctc
     6661 cggcctggtg aagcaggagc ggggaaaaat cttccttacg gaaggaatcg gcccggacac
     6721 cgcgaagctg ctgccgcgcg agccgtaccg cgtggtgaag ctcggcgtgg tgcaggttcc
     6781 cgaggggcgg caggtctttc ccggcctcac cgtccgcgag aacctgatgg cgggcgcgta
     6841 caccgtgaac gacgcggggc gcgtccgcag aaacctcgac cggatgttcg agatgtaccc
     6901 catcctgaag gagcgcgctc ggcagcaggc ggggacgctc tcgggcgggg agcagcagat
     6961 gctcgccatc tgcagagggc tgatggcgga gccgaaggtg ctgcttctcg acgagccgtc
     7021 gctcgggctc gccccgatca tcgtgaaagg ggtcttcgac acggtgcggc gcatccgcga
     7081 cgaggggatc accgtcgtgc tggtggagca gaacgccaac caggcgctcg ccatcgccga
     7141 ctacgcctac gttctggaga acgggcgcgt ggcgctcgaa ggcgaggggc gcgcgctgct
     7201 ctgcgacccc gccgtgaagg acgcctacct cggcggggcg caggaagcct gatccgccgc
     7261 tattgcagta agagagaagg aggaaggatc atggtcaaca aactcttcgt gcagcctctt
     7321 cttttcgcct cccccgtcta ctggcccgac agggcggagc tggacgccgc agggaaggcc
     7381 gtcgccggaa tgctggaggc gatggacgcg ccaacgtcgc tcatgcggac cgcgcgcatc
     7441 gtggcgggag acaccgacct cgacgacgcg ctctcttcgc tcgggcgggc ggttcctctg
     7501 ctcgttcccc tctcgggggg cgtgcagaaa cggatgctcg ccgccgccgg agcggcctcc
     7561 gaaagcctgc tctggagcta cttccccaac gacgacatct atccggagga ggagcggccg
     7621 acgattacgc gcatcgtctc gaagaacgcc ctccccgccg tcgccgacgt ctggggaacg
     7681 ctccgcaaca gggggtgcag agtggcgaag gtctaccacg agggctcctg gctggccgct
     7741 gcgagggagc tgaaagccgc gcacagggtc cggagcgcgc gtctgctgct ggtggggtac
     7801 acgcaggagt gggtggtctc cgcgagcgtg gacacgcgga agatggagcg gctgctgggc
     7861 atttcgtcgg tccatatcgg gctggaagag ctgttcgcgg agttcgaagc gtgcggaaac
     7921 gattccgtcg cggcctcgct cgcgaaggag ttcgccgacg gcgcgcgttc ctgcctggag
     7981 ccgaacatgg agcagatcga acaggcgttc cggatgtacc gggcgctgaa gaacctgctc
     8041 gcccgctacg gctgcaacgc gctcgccatc tcctgcttct cgctggcgaa aacgctgggc
     8101 gtcacggcgt gcgtcgcgct ctcgatgctc aacgacgaac cggagaccgt ggccgcctgc
     8161 gagggggacc tggactccgc cgtctccatg atcgtcggca aggcgatcac cgggatgccc
     8221 gtcttcatgg ggaaccccgt gtataacttg gagaacacgc tcgacatcgt ccactgcacc
     8281 gcgccccgga agctcctctc cgcgaacgcg cagccgtacg agatccgctc gcaccacgag
     8341 acggggctct ccgtctcgca gcgggtggag gtggaggggg agcgcgacgc gaccatcttc
     8401 cgcatcggca acgagttccg gaacgccgtg ctcttcgagg cgcccctgct ctcgaacccg
     8461 aggctcgaca cctgccggac gcagttccgc ttcggcctga agagctggga ggcgtgcttc
     8521 gacgccatgc tcggctgcca tcacatggtc gccttcggcc gccacggcga cgccctcgag
     8581 cgcttcatga agcagcgcct cggcctggag gttgttcgtt tctgacggca gccgccacga
     8641 gatacgcaag cgcccccgga gaggttttcc aggggcgttt gcgttcttcc acctctatga
     8701 tgttcttgac gagcgtgaac ccgcaatatc gcgtttctcc tgcatccgct gccgccgccg
     8761 ctcgaaatga gcgaccgcag gcagcggtta aatcggtccc gggcaggacg tttgagacgg
     8821 tattcttccc gtgataactt cccgaatcga cgatgcatct tcgaccgccc tgcgggcgtc
     8881 ggattcggca agttcgattt tggaggggta cctcgggacg gtgccgaatc gattcaaccc
     8941 gacgatttcg ctcgccatgc cgtcccatat ctccgctgcg atcttctttt ctttgcatat
     9001 ccgggcaaga tcgataaggt tatgggtttt aggcggctct tcgccggatg aaaccataag
     9061 ggcctttata tatttctcgg cggactgctg actgtgataa cagatgattt caacgggta
//
LOCUS       DAOPKU010000038         9100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8052_length_9100_cov_5.208955, whole genome shotgun sequence.
ACCESSION   DAOPKU010000038 DAOPKU010000000
VERSION     DAOPKU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9100
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8052_length_9100_cov_5.208955"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PK849_02100"
     CDS             <1..1473
                     /locus_tag="PK849_02100"
                     /inference="COORDINATES: protein motif:HMM:NF037272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HPE64944.1"
                     /translation="DVLPVGRRPSPLNLEHLKHPEAGASARTKRALPAKYDLRTLNRL
                     PPIRDQGLYGSCWSFASFVALESPLLPGEVRDFSEWHLAYWTFVTNPEGFPAFTAWSD
                     PNDPFSPDDPAKVYNQGGDDWKTVAVLVRGTGPVNEADLPYGGPLPSTKPNLPREKLL
                     TGALYMPYSASEIWEQGYPYIVADEVKASIIKYGALSIGMMADFNENTWNTETHAFFY
                     NGPRGANHAVNVVGWDDGFPKENFATTPPGDGAWIVRNSWGEEFGEGGYFYMSYYDTS
                     VDSGIAYILASPDLYGIIHQHDPLGWVRSVSASEANPETGWMANIFTAGEDQTLRAVS
                     FYAAAYGTMYEVFIDDVSGGVSGSGEALRTAEASGTLEQPGYHTIPLPRPVGVGEGTR
                     FRVRVKLTTPGYEYPLPVELPLEEYSDNATASPGESFYSADGASWTDLTEDFAEANFC
                     IKVLSTPGMSRSGGSGGCSAASASPLLFALLAPLLLLRRR"
     gene            complement(1564..3252)
                     /locus_tag="PK849_02105"
     CDS             complement(1564..3252)
                     /locus_tag="PK849_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE64945.1"
                     /translation="MEKKKVLIIGGVACGAKTAARLARLAPEFDITILERGEHLSVAG
                     CGFPYFVGDVVKEYKDLVCTPLGIVRDANFFRNVKNIAVHTGHLATRIDREKKVVAAT
                     DTASGEERLFPYDELVLATGASPIRPPIPGNDLSRVFTLWTMPDAMAMKSAIDAGSVK
                     SAVIIGGGLIGMEVVEALVDRGISVSVADIQPTPLPALIGEDFGLRVTKVLRQKGVGF
                     YGGEKVVEIIGENGVVTGVRTDKRTLPADMVLLAVGIRPNTILAKEAGLELHPNGTIV
                     VDECLRTSDPAIYAGGDCVQSKNILTEKSVWQPMGSTANRQGRVIADNIAELPTRFAG
                     VVGTAILKLFHLTIGKTGLNEEQAHAAGFDAVSIVVEEPDRPHFMPGMGAMTIRLVAD
                     RKSRRIVGAQIMGGGVVDKRLDSIVAAISAGMTVDMLADTDFAYAPPFATALDPTTHA
                     ANALRNKMDGLVKTYSAKELKALMEGANPPFLLDVRSPQELDLQGRIPYDFANIPLGQ
                     LRGRITEIPKDREIVTFCKISVRGWDAIATLRGNGYENVALLEGGIMAWPYDLR"
     gene            complement(3369..3599)
                     /locus_tag="PK849_02110"
     CDS             complement(3369..3599)
                     /locus_tag="PK849_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPE64946.1"
                     /translation="MHCLATFDITSMAVLFERVCRKEGITAKIVPVPRSLSTSCGLAC
                     EFPCEEEERVRELCGKKHVDVTDFHSLGDDWT"
     gene            3747..3923
                     /locus_tag="PK849_02115"
     CDS             3747..3923
                     /locus_tag="PK849_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64947.1"
                     /translation="MRFLLLALVLIAACIPMLLGSERVRPCYKGCHGCGKCMKSFRGD
                     GENEERTPQPPANR"
     gene            complement(4001..5296)
                     /gene="larC"
                     /locus_tag="PK849_02120"
     CDS             complement(4001..5296)
                     /gene="larC"
                     /locus_tag="PK849_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HPE64948.1"
                     /translation="MKTLYLDCFAGIAGDMFLGAMLDLGLDREAFLRTMESVVLFPGH
                     EHGHAHPDPHDHEHEHSHEHNDTHHHAHHHSHGTDRLRIAITQTVRGGIAGTKVNIAS
                     SEDHPHRGLADVLEIIGASPLSPRVKERSAAAFRLLAEAEAKVHGTTPDKVHFHEVGA
                     LDSIADIIGAFVLVEMAGVDAVVSSPLNVGHGTITCAHGILPVPAPATAELLAGLPVR
                     VEGGPMERVTPTGALLVRCLASSFGPLPAGKVLGSGKGAGDRDGDIPNVLRVLLLETE
                     ATNTDGAGNIARDSGIVIETNIDDMNPQYYAPVMNRLLAEGALDVWVAPIVMKKGRPA
                     ITLGCLCRPGAERSLAETLLRETTTLGVRMYAVDRLKADAEIVERETSWGIVRFKVAR
                     LGEEVLRSNPEFEDVRRLSEEHGIPLPEMRERLMNEFLL"
     gene            complement(5457..6005)
                     /locus_tag="PK849_02125"
     CDS             complement(5457..6005)
                     /locus_tag="PK849_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64949.1"
                     /translation="MTVTLSPVLSFRRGGASAGSSPHCLKGLFLLLLLFFCFAPILSP
                     LPADAKGPEEIVFLKGLFVKEVRGYGLYVEEESNRFHKDDKPTIYLEVDNFKLGVKDE
                     QYHISLSLDLLVKDEKGAVVAEDKQIITHEYFLKSRVRDVWFTVILDLSGWPEGKHIL
                     SWVVTDNTSGKQCTKDMEIEIH"
     gene            6174..6884
                     /locus_tag="PK849_02130"
     CDS             6174..6884
                     /locus_tag="PK849_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HPE64950.1"
                     /translation="MKTPSSTPENHYLSLDADWGEALARIPESSSKKVSGPFRGHFDG
                     ASRGNPGEAGAGALLLDASGTPVWTCARPLGRKTNNEAEYAALILLLEEILARGLAGV
                     SVRGDSQLVVNQVTGRWKVKEPRLQELAERAKELLKATKSSLSWVPREENKDADALSN
                     EALDGPAKPAQSALTGKGADDTAAFPAERLERVTGSIYIAHGSEDYAVDLLHKACTCP
                     SFQHRKRCKHLEAAERLA"
     gene            complement(6910..7728)
                     /locus_tag="PK849_02135"
     CDS             complement(6910..7728)
                     /locus_tag="PK849_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64951.1"
                     /translation="MNRVLLDIRKLTIDFPPSRKRKAVRALKEFSLRLEAGESVSIVG
                     ESGSGKTTLIRCISGHVTPVSGSVSLFGADLSEASNQELVALRRRCGVVSQDPYGSLP
                     PTLTVLEAVMEPWLLVRGREARQAGEHKARELLDELGIGREEILSSKVRLSLSGGQRQ
                     RVAIARALVLGPELLLCDEPTSMQDASTRGDILRILQRRQETGMSMIFVTHDLFLARK
                     AAPRGLVLYRGEAVEEGLCRDFLASPQHPYTKALVAALPKLSSSATYAGESGGR"
     gene            complement(7725..8519)
                     /locus_tag="PK849_02140"
     CDS             complement(7725..8519)
                     /locus_tag="PK849_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64952.1"
                     /translation="MISIRNLSITYGRMTSRGRSGETEAVKNISFSVEKGKLFALAGE
                     SGSGKSSVLMAIPGLLPEGTRVSGEILLDGRNLTALSEEELNALRWRRIALIPQGAMN
                     SFTPVLTVGRHIEEVLDVHLGLSKRAARERTQELFVRAGLDGALASRYPHELSGGQKQ
                     RAAIALALACDPDWLLCDEPTTALDVITQKGIVDTLKDLVTTKGVGMLLVSHDLPLAA
                     SVCSRLFIMKGGTIVEEGAPREIVAHPKNPHSAALVNALLELEREE"
     gene            complement(8516..>9100)
                     /locus_tag="PK849_02145"
     CDS             complement(8516..>9100)
                     /locus_tag="PK849_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64953.1"
                     /translation="TFLGTSFGLAAGYLGGWTDSILMRIVDLLLSIPLLPVLMALAAL
                     WGKGLWQLVLILSVFSWMGTARTVRALVLTLRDAPYIEGLRGLGAPSGYILRKHLLPE
                     TLPVLLATMTLGVPGAILSEAGLSFLGLSDPRVISWGRMLHEAHSFGAFTAGAWWMLL
                     PPGLGIALLCLVFLDLGKFLEENVDPRLRKGVRP"
BASE COUNT         1670 a         2781 c         2922 g         1727 t
ORIGIN      
        1 gatgtgctgc ctgttggaag acggccgtcg ccgctgaacc tggagcatct aaagcatcct
       61 gaggcagggg cgtccgcgcg gacgaagagg gctctccctg cgaagtatga cctgcggacg
      121 ctgaacaggc ttcctcccat ccgggatcag gggctctacg gttcctgctg gagcttcgcc
      181 tccttcgtcg ccctggagtc gcctcttctg cccggagaag tccgggattt ttctgaatgg
      241 catctggcat attggacgtt tgtgacgaac cccgaaggct tccccgcatt tactgcgtgg
      301 agcgacccga acgatccatt ctcccccgac gatccggcga aagtctacaa tcaggggggc
      361 gacgactgga agaccgtggc ggtgcttgtc agagggacgg ggccggtgaa cgaggccgac
      421 ctcccttacg gagggcctct tccgtcgacg aaaccgaatc ttccgaggga aaaactgctg
      481 accggagcgc tttacatgcc ctactctgca agtgaaatct gggagcaggg gtacccgtat
      541 atcgtcgcgg acgaagtgaa ggcgtccatc ataaagtacg gcgcgctctc catcggcatg
      601 atggcggact tcaacgagaa tacgtggaat actgaaacgc acgctttttt ctacaacggc
      661 ccccgtggtg cgaatcacgc ggtgaacgtg gtcggatggg acgacggttt cccgaaggag
      721 aacttcgcga cgacgcctcc gggagacggt gcgtggatcg tccggaacag ctggggagaa
      781 gaattcggcg agggcgggta cttctacatg tcctactacg atacgagcgt cgacagcggg
      841 atcgcgtaca ttctcgcttc ccctgatttg tacggcatca tccaccagca cgatccgttg
      901 gggtgggtgc gctcggtgag cgcctcggag gcgaatcccg agacagggtg gatggcgaac
      961 attttcaccg ccggggagga tcagacgctc cgcgcggttt cgttctacgc ggcggcgtac
     1021 ggaacgatgt acgaggtgtt catcgacgac gtatcgggcg gcgtctccgg ttcgggggaa
     1081 gcgcttcgta cggcggaggc ttccggtacc ctggagcaac cgggatacca tacgatccct
     1141 ctgccccggc cggtcggagt gggagaagga acgcgcttcc gcgtgcgggt gaagctcacc
     1201 acgccggggt acgagtaccc cctgcccgtc gaacttccgc ttgaggagta ttcggacaac
     1261 gcgacggcct cccccggcga gagcttctac agcgcggacg gcgcttcgtg gacggatcta
     1321 acggaggact tcgcagaggc gaacttctgc atcaaggtcc tctcgacgcc cgggatgagc
     1381 agaagcggcg ggagcggcgg atgctccgcc gcctccgcgt cgccgctgct tttcgcgctg
     1441 ctggcgcctc ttctgctgct ccgcaggagg tagagccaaa aggctgttac gcaaaaagaa
     1501 gatgcgggtt cccggagttc tcgggggccc gcatcttctc tttgcatgta atactcgggt
     1561 tttctaccgt aaatcgtacg gccaggccat gatgccgcct tcgaggaggg cgacgttctc
     1621 gtagccgttc ccgcgaagcg tggcgatggc gtcccatccg cgcacggaga tcttgcagaa
     1681 cgtcacgatc tcgcggtcct tcgggatttc cgttatacgg ccgcgaagct gtcccagcgg
     1741 gatgttcgcg aagtcgtagg ggatgcgccc ctgaaggtcg agctcctgcg gggagcgaac
     1801 gtcgagcagg aacggagggt tcgccccctc catgagcgcc ttcagctcct tcgccgaata
     1861 ggtcttcacc agcccgtcca tcttgttccg cagcgcgttg gcggcgtgcg tcgtggggtc
     1921 gagcgcggtg gcgaaggggg gggcgtaggc gaagtcggtg tcggcgagca tgtcgaccgt
     1981 catcccggcg gagatcgccg cgacgatgga gtcaagccgt ttgtccacca cgccgccgcc
     2041 catgatctgc gcgccgacga tgcggcgtga ctttctgtcg gcaacgaggc ggatcgtcat
     2101 cgcgcccatg cccggcatga agtgcggccg gtcgggctcc tcgacgacga tactcacggc
     2161 gtcgaatccc gccgcgtgcg cctgctcctc gttgaggccg gtctttccga tggtgaggtg
     2221 gaagagcttc aggatcgcgg taccgacgac tccggcgaaa cgcgtgggca gttccgcgat
     2281 attgtcggcg atgacgcgcc cctggcggtt cgcggtggag cccatcggct gccagacgga
     2341 cttctcggta aggatattct tggactgaac gcagtcgcct ccggcgtaga tggccgggtc
     2401 gctcgtgcga aggcattcgt cgacgacgat ggtcccgttg gggtggagtt cgagcccggc
     2461 ttctttggcg agtatcgtgt tcgggcgaat gccgacggcc agcagaacca tgtcggcggg
     2521 aagggtgcgc ttgtcggttc tgacgccggt gacgacgccg ttctcgccta tgatttcgac
     2581 gaccttctcg ccgccgtaaa agccgacgcc cttctgacgc agcactttcg tcacgcggag
     2641 gccgaaatcc tctccgatga gggccgggag gggggtgggt tggatgtcgg ctacggagac
     2701 cgagatgccg cggtccacaa gcgcctcgac gacttccatg ccgatgagcc ccccgccgat
     2761 gatgacggcg gacttgacgc ttccggcgtc gatagccgac ttcatcgcca tcgcgtcggg
     2821 catcgtccag agggtgaaga cgcggctgag gtcgtttccg ggaatgggag gacggatggg
     2881 ggaggcgccg gtggcgagta cgagctcgtc gtaggggaag agacgctcct ctccggaggc
     2941 ggtgtcggta gcggcgacga ccttcttttc cctgtcgatg cgcgtcgcca agtgtcccgt
     3001 gtggacggcg atattcttca cgttgcggaa gaagttcgcg tcgcggacga tgccgagcgg
     3061 cgtgcagacg agatccttgt actccttcac cacgtcgccg acgaaatacg ggaagccgca
     3121 tccggcgacg ctcagatgtt cgccccgctc cagaatggtg atgtcaaact ccggcgcaag
     3181 tcgcgcgagc cgggccgccg tcttcgcgcc gcatgccacg ccgccgatga tgaggacctt
     3241 ctttttctcc atgaccacaa tcccctgcct ttcggaaatt cgcggcccgg cggacgtatg
     3301 ttcagcgagc ggacgccctg acgccgcgat ttatactgct acccctatat ggtatttcaa
     3361 aaaggaagtt atgtccagtc gtcccccaaa gaatggaaat cggtcacgtc cacatgtttt
     3421 ttcccgcaga gctcgcggac gcgctcttcc tcttcgcagg ggaattcgca ggcaagtccg
     3481 cagcttgtgg aaaggctgcg cgggacgggg acaattttgg cggtaattcc ctctttgcgg
     3541 cagactcgct cgaagaggac ggccatgctc gtgatgtcga aggtcgccag acagtgcatc
     3601 gctaccgcct cctttcgtga tgaggaatgt gccggcaaat ggccggttcg tgcgtcctgg
     3661 ttcgttcaaa gcgcatgcaa acacaacctt tcaaaaagag cgtatactat gggctgaaaa
     3721 aaactctttc ccggaggtga cgtcgtgtgc gttttcttct gctcgcgctg gtgctgattg
     3781 ccgcctgtat cccgatgctg ctgggatcgg agcgggtccg cccctgctac aaggggtgtc
     3841 acggctgcgg aaagtgcatg aagtccttcc gcggcgacgg ggagaacgag gagcgcacac
     3901 cgcagcctcc cgcgaatcgc tgacgactgc tgcggacgcg acggcgttcc ggactcctga
     3961 acagacggct ctctccggtc cgaatgcggc gcgccgcttt tcagaggagg aactcgttca
     4021 tcaatcgttc ccgcatctcc ggaagcggga tgccgtgttc ctccgaaagt ctccggacgt
     4081 cctcgaactc agggttgctc cgaagaactt cctctccgag gcgcgccacc ttgaagcgga
     4141 cgatacccca cgaggtctcc cgctccacga tttccgcgtc ggccttcagc ctgtcgaccg
     4201 cgtacatccg gacgccgagc gtcgtcgtct cccgcagcag cgtttccgcc aagctccgct
     4261 ccgcgccggg ccggcagagg cagccgagcg ttattgcggg gcgtcctttt ttcatgacga
     4321 tgggcgcaac ccatacgtcg agcgccccct ccgcgaggag cctattcata accggcgcgt
     4381 aatactgcgg gttcatgtcg tcgatgttcg tctcgatgac gattcccgaa tcccgcgcga
     4441 tattccccgc gccgtcagtg ttggtcgctt cggtctcaag cagcagcacg cgcagtacgt
     4501 tggggatgtc gccgtcgcgg tcgcccgccc ccttgccgct tccgagcacc ttccccgcgg
     4561 ggagcggccc gaacgaggaa gcgaggcatc gcacgagcag cgctccggtt ggggtgacgc
     4621 gctccatcgg ccctccttcg acgcggaccg gaagaccggc aagcagctct gcggtcgccg
     4681 gagccgggac gggaaggatg ccgtgcgcgc atgtgatggt tccgtgcccg acgttcagcg
     4741 gcgaggagac gacagcgtcc acgcccgcca tctccacgag gacgaacgcg ccgatgatgt
     4801 cggcgatcga gtccaatgcc ccgacttcat ggaagtgtac cttgtccgga gtcgttccgt
     4861 ggaccttcgc ctcggcctcg gccaaaagac gaaaggcggc ggcgctccgc tccttcacac
     4921 gcggagagag cggacttgcc ccgatgattt cgaggacgtc ggcgagccct cggtgcggat
     4981 gatcctcgga cgaggcgatg ttcacctttg ttcccgcgat gccgccgcgg accgtctgcg
     5041 taatggcgat gcgcagcctg tccgtgccgt gcgaatgatg gtgtgcatga tggtgtgtat
     5101 cgttgtgttc gtggctatgc tcgtgttcgt gatcgtgcgg gtcggggtgc gcatgaccgt
     5161 gctcgtggcc gggaaagagc acgacgcttt ccatcgtgcg gagaaacgcc tctctgtcga
     5221 gcccgaggtc gagcatcgcg ccgaggaaca tatcgccggc gattccggcg aaacagtcaa
     5281 gatagagtgt tttcatcaaa agacctccgt gtgcaaagtt cacaacgcgg ttattgtatc
     5341 atctgccgga cggaatacat cgggaggtga gaaaaacggc gcgccgtccc cacgcaaaaa
     5401 gccgccgggc gaacggatac atccggcggc gctgtgttct ccttcacgag aaaaggtcag
     5461 tggatttcga tctccatatc cttcgtgcac tgcttcccgg aagtgttgtc ggtgaccacc
     5521 caggagagaa tatgcttccc ctccggccac cccgagaggt ccaggatcac cgtgaaccag
     5581 acgtcgcgaa ccctgctctt caggaaatac tcgtgcgtga tgatttgctt gtcctcggcg
     5641 acgacggcgc ctttttcgtc tttgacgagc agatcgagcg aaagactgat atgatactgt
     5701 tcgtccttca ctcccagctt gaagttatcg acctcgaggt agatcgtcgg tttgtcgtct
     5761 ttatgaaagc ggttgctctc ctcttcgacg tagaggccgt atccgcggac ctccttgacg
     5821 aaaagcccct taaggaagac gatttcttca ggacccttgg cgtcggcggg aagaggcgag
     5881 agaatcggcg cgaagcagaa aaacagaaga agaaggagaa aaagaccttt aaggcagtgc
     5941 ggcgagcttc ctgcggaagc gccgcctctc cggaaagaaa ggactggcga tagtgtcaca
     6001 gtcatgcagc ctccttgaga taaaaatgaa aagcccaaag cattatatag atttttttat
     6061 tcatggacta gggaaatata ctgtttatcg aaaatacatc gtgcaaatga atcttaaggc
     6121 ttatttgttc tcgaaaatta acgaaagtta cctttgctga taaggagcat cctatgaaaa
     6181 caccttcgag tacccctgaa aatcactatc tgagcctcga cgcggactgg ggcgaagccc
     6241 ttgcccgcat tccggagtcc tcttcgaaaa aagtttccgg ccccttccgg gggcatttcg
     6301 acggcgcttc ccggggcaat cccggcgagg ccggcgcggg tgcgctgctg ctcgacgcgt
     6361 ccggaacgcc tgtatggacg tgcgcccgtc cgctcgggcg gaagaccaac aacgaggcgg
     6421 agtacgccgc gctcatcctg ctgctggagg agatactggc gcgcgggctc gcgggcgtct
     6481 ccgtccgggg ggacagccag cttgtggtga atcaggtcac cggacgctgg aaggtgaagg
     6541 agcctcgtct gcaggagctt gccgagcgtg ccaaggagct gctcaaggct acgaagagct
     6601 cgctctcgtg ggttccgcgg gaagagaaca aagacgccga cgcgctctcc aacgaagcgc
     6661 tcgacggtcc tgcaaagcct gctcagagcg ctttgacggg gaagggcgcg gatgataccg
     6721 ccgcatttcc ggccgaacgc ctggaacggg ttaccggctc catctacatc gcccacggaa
     6781 gcgaagacta cgccgtcgat ctgctccaca aggcgtgcac ctgtccttcg ttccagcaca
     6841 ggaagcggtg caaacacctg gaggctgcgg agagattggc gtagcgcgcg ccgtgaaccg
     6901 gccgcgccgt catcttccgc cggactcccc ggcgtacgtc gctgaagatg agagtttcgg
     6961 aagcgccgcg accagcgcct ttgtgtacgg gtgctgcggc gaagcgagaa agtcgcggca
     7021 gagcccctct tcgaccgcct ctcctcggta gagcacgagc cctctcggcg cagccttccg
     7081 cgcgaggaag aggtcgtggg tgacgaagat catcgacatc cccgtctcct gccgccgctg
     7141 gagaatccgc agaatatccc ctctcgtcga ggcgtcctgc atgctcgtcg gttcgtcgca
     7201 gagcaggagt tccggtccga gcacgagcgc cctggcgatg gcgacccgtt ggcgctggcc
     7261 gccggagagg gagagcctga ccttggacga caaaatttcc tcccggccga ttcccagttc
     7321 gtcgagcagt tcccgcgcct tatgctcccc ggcttgccgc gcttcgcgtc ctctgacgag
     7381 aagccacggc tccatcaccg cctccagcac ggtgagggta ggagggagcg agccgtacgg
     7441 atcctgcgag acgacgccgc atcggcgtct gagcgcgaca agctcctggt tcgaggcttc
     7501 ggagaggtcg gcgccgaaga gcgacacgct ccccgagacc ggcgtcacgt gtcccgagat
     7561 gcagcggatg agcgtcgtct tcccgctgcc ggactcgccg acgatcgaga cgctctcccc
     7621 ggcctcaagg cgaagagaga actccttgag cgcccgcacc gcctttcgtt tccgcgaggg
     7681 cggaaagtct atcgtcagct tccggatgtc gagcagcacc ctgttcactc ctctctctcc
     7741 agttcaagca gcgcgttcac cagcgccgcc gagtgggggt tcttcggatg ggcgacgatc
     7801 tcgcggggcg ctccctcctc gacgatcgtt ccccccttca taatgaagag gcgcgagcag
     7861 accgacgcgg caagggggag gtcgtgcgag acgaggagca ttccgacgcc cttcgtcgtg
     7921 acgaggtctt tcaacgtgtc gacgattcct ttttgtgtga tgacgtcgag cgccgtggtc
     7981 ggctcgtcgc agagcagcca gtcggggtcg cacgcgagcg cgagcgcgat ggcggcccgc
     8041 tgcttctgcc ctcccgaaag ctcgtggggg tatcgggatg ccagggcgcc gtcgagtccc
     8101 gctctgacga acagctcctg cgtccgttca cgcgccgctc tttttgagag cccgaggtgg
     8161 acgtcgagca cctcctcgat gtgccgcccg acggtgagca ccggcgtgaa ggagttcatc
     8221 gccccctggg gaatcagcgc gatgcgcctc cagcggaggg cgttcagctc ctcctcggag
     8281 agcgccgtca ggttcctccc gtccagcaga atttcccctg aaacgcgcgt tccctccggc
     8341 agcagccccg gaatcgccat gagcacggag ctctttccgc ttccggactc tccggccagc
     8401 gcgaagagct ttcctttttc gacggagaac gagatgttct tcaccgcctc ggtctcgccg
     8461 cttcgtccgc gggacgtcat tcgtccgtac gtgatcgaga ggttgcgtat ggagatcatg
     8521 ggcggactcc tttccggagc cgggggtcga cgttctcttc aaggaatttt ccgaggtcga
     8581 gaaagaccag gcagagcagg gcgatgccga gcccgggagg gagcagcatc caccacgcgc
     8641 cggcggtgaa cgcgccgaag gagtgggctt cgtggagcat cctgccccag gagatgaccc
     8701 tcgggtcgga gagcccgagg aaggagagtc ccgcctcgga gagaatggcg ccggggacgc
     8761 cgagcgtcat cgtcgccagc agcaccggga gcgtctccgg aagcaggtgt tttcgcaaaa
     8821 tgtaccccga gggcgcgccc aggcccctca gcccctcgat gtacggggcg tcgcggagcg
     8881 tgaggacgag ggcgcggaca gtccgcgcgg tccccatcca cgagaagacg gagaggatga
     8941 ggacgagctg ccacagcccc tttccccaga gcgcggcgag cgccatgagc accgggagca
     9001 gcgggatgga gagcagcagg tcgacgatgc gcatcaggat gctgtcggtc cacccgccaa
     9061 ggtaccctgc tgcgagtccg aaggaggtcc cgaggaaggt
//
LOCUS       DAOPKU010000039         9082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8069_length_9082_cov_4.160851, whole genome shotgun sequence.
ACCESSION   DAOPKU010000039 DAOPKU010000000
VERSION     DAOPKU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8069_length_9082_cov_4.160851"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="PK849_02150"
     CDS             complement(<1..1199)
                     /locus_tag="PK849_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE64954.1"
                     /translation="MGLVLAGSFAFFLLLGVPVGLAIGVSGLLIVFLNGFPLEMIPQT
                     MFSGNESFALVAVPFFVLAGDILAKGGISERIIAFAEATLGRFRGGLSIVSTVASMFI
                     AAISGSGAATTAAVGSALLPQLEEKGYDVDFSAALIAASGTIGVVIPPSVPMVLYAVI
                     AGVSVAKLFMAGFVPGFLMGLGLIVYSIYVARKRGYPASRSTTGREKWKLFLDATWGL
                     MTPVIILGGIFSGFFTPSEAAAVAVVYSLFVAFFIYRSLSFKQFYKIVVGSGVTSALI
                     MFIIATAKLFGWGLAFFQIPEAVARFLLGFAGGNTFMIYFMITIIILLAGMFMETASA
                     LIVLTPIFLPTIIRTGGNLLHFGVLIAIGLAIGMATPPVAINIYVAGAITGLPMEQIS
                     KPIIPMV"
     gene            complement(1201..1710)
                     /locus_tag="PK849_02155"
     CDS             complement(1201..1710)
                     /locus_tag="PK849_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE64955.1"
                     /translation="MNSRDNGTRGGSVFSPLVRLSDFVNKISEILLFAMMLAMIAVTT
                     MQIVCRVFFEALIWSEELTTYLLVAASLLGAAIAFKRGSHIAVTFLVHKLPLPLRKVA
                     EFLVQGIGILFFFVVAYYGGVLMKSEAMQTTPAMGISMTWIYLMYPVVGSIVLLHLVA
                     GLGGILGRK"
     gene            complement(1722..2696)
                     /locus_tag="PK849_02160"
     CDS             complement(1722..2696)
                     /locus_tag="PK849_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPE64956.1"
                     /translation="MKRSIAVVSIVLLTLLFVASAAVAAVDIKLAHVVNENDAFHVCA
                     LKFKELVEAGSKGELTVTIYPNAQLGDERNLLESMRMGVVDSAIITGGPIMNFMPKFG
                     VFDLPFLFSTPEHAYKVLDGEIGKMMLNEMEQHGWKGLAYGERGFRNLTNSKQPVKTP
                     EDMKGLKIRLMQNPVYVDSFRALGANAIPMAWTEALTALQQGAIDGQENPLNVIVAFN
                     LFESQKHLAITRHAYAPNVIMMSKKTWEKLAPEMQSLVQDAAQKAAEHNRAYDNEKEA
                     EWLQFLKDKGMEVTETDTAAFREAVKPVYQKYEEQFGKELIEAILADK"
     gene            3010..3393
                     /locus_tag="PK849_02165"
     CDS             3010..3393
                     /locus_tag="PK849_02165"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE64957.1"
                     /translation="MADFVLLAGKISDIRLAEDPSHASGIEKRIVFAPGKFWNDYVAR
                     HFIVKGKNSKTPFHTHDWPHYVFILSGRCNATIDGTVYPLEKGCWAHVPPNVEHFFES
                     TGEEPLDFICIVPPKGDPAGSAHLA"
     gene            complement(3475..3942)
                     /locus_tag="PK849_02170"
     CDS             complement(3475..3942)
                     /locus_tag="PK849_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE64958.1"
                     /translation="MTYLNYVFFLVFWGWILSVGYIRYILPFIESAFDTLEAMEKSGE
                     LFPRAIAFIAKLAMTGSQMYILGIWSAYCVLRTMIFLHEPGTNGWLYYITAFLVCEGF
                     LGAVAKKEKYRGLLSVFHSAMAMGLFVIFAMNPYFLRSVYPWLPPMMNFSFPH"
     gene            complement(4093..4569)
                     /gene="gpt"
                     /locus_tag="PK849_02175"
     CDS             complement(4093..4569)
                     /gene="gpt"
                     /locus_tag="PK849_02175"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HPE64959.1"
                     /translation="MSSERYHKTYPVSWEQVHRDCKALAWRLVSVCKWERIIGIARGG
                     LIPAAIIARELDVRLVDTVCVSSYVLRTQGEPSVLKEISGDGAGWLIIDDLVDTGKTA
                     QVVRRMLPKAHFAAVYAKPDGRAFVDTYITEVSQDTWVLFPWDAETAYAKPIVEQR"
     gene            4744..5829
                     /locus_tag="PK849_02180"
     CDS             4744..5829
                     /locus_tag="PK849_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE64960.1"
                     /translation="MKRFVVLLVFVLSVTLCGAAYAAFPAIPAEKINPGFVYIGPIGD
                     GGWTYMHEQGRLAMEKEFPGVKSTFVESIPEGPDAARVFETMIRNGSKVIFGTTFGYM
                     DFVQEVAKKYPDTIFMHCAGYKDAPNVGTYFGRMYQARYLSGLVAGKMTKKNRIGYVG
                     AHPIPEVIRGINAFTQGVRKANPEAEVQVIWIFSWLDPGKEKEAAKALIDSGVDILAM
                     HADTGATPQAAEEAGIYVIGYNNDMSAYAPTKHLTAPIWDWGMVYKETIGQVIKGTWQ
                     PSHIWYGLKEGMVALAPYGKDVPDLVKALVDVEKAKIVSGEWDVFHGPVKDQSGEVKV
                     PEGAAMTDGEMLSMNWFVEGVKGDIPK"
     gene            5997..7565
                     /locus_tag="PK849_02185"
     CDS             5997..7565
                     /locus_tag="PK849_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE64961.1"
                     /translation="MDARAPSKPLVRMEKITKRFTGVTANLDVDFDVRPGEVHALLGE
                     NGAGKSTLMNILYGLYRPDSGVLTIEGKDVSFSSPRDAIASGIGMVHQHFMLIPSQTV
                     WENMILGLEGLSSVLPKRAIRERILELSSRYGLSVDPDAKIWQLSIGEQQRVAILQML
                     YRRAKILVLDEPTAVLTPQEAEHLFVTVRQMTSEGHGIVFISHKMDEVMGLSHRVTIL
                     RKGRLVGTVDTASTTKEKLAEMMIGQKMIFSIEKKELQPGKPVIECASVCALGDRGIP
                     AVSRATFMIREQEILGVAGVAGNGQQELCETLVGLRKVESGAIRVNNEELTNSIPKTF
                     IDKGIHYIPADRKGTGLVPNMNVSENSILKRYWSRAVSKKGFIDWKEVFSFARGLVSR
                     YNVSTPSMETPVRNLSGGNLQKLMLGRELSDSPIAILAVHPTWGLDVAATKFVREQLL
                     AAREAGCAVLLVSEDLDELLAMSDRLAVMCKGEIMGILDSPAETPVEAIGLMMAGTPA
                     EEALASAPRGGEKS"
     gene            7562..8671
                     /locus_tag="PK849_02190"
     CDS             7562..8671
                     /locus_tag="PK849_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64962.1"
                     /translation="MKLSIKVEPRLETPRMLGIAIPVISLAAALLSGAVFLFFMGVSP
                     FQAYSTIIRSSLGDAYGLSETLVKAIPLAMAAIAVMLSFTMLIWNIGAEGQIFMGAIA
                     AAAAVRYLFVDNHLAMFAIMFAAAGIAGGIWAAIAGYFRARWSVNEIITTLMLNYVAM
                     NLLNYFVYGPWRDPASLGFPMTAPFPDAARLPSFWGTRIHAGLFLAILFPLIFWVILR
                     FTRWGYEIRVMGENPKAAGFAGMSYIKNVVFVMFLSGAIAGIAGMCELSGLQGRLQHG
                     FSGGYGYTAIIVAWLARLHPIAIILVSFLMGVLLVGGETLQIVMRLPLSSMLVLQGLI
                     LFFVLGGEFFRKFRIRILSGGATPPIATTSAKEEK"
     gene            8673..>9082
                     /locus_tag="PK849_02195"
     CDS             8673..>9082
                     /locus_tag="PK849_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE64963.1"
                     /translation="MEILIPILTAAVRSGTPILYATLGEIVTEKGGILNLGLEGIMLL
                     GAYAGFAVSYSTGNAWLGIAAAFFVGTLLGLVHAFICVTLKGNQVVSGLAMTMFGTGA
                     SSLLGRNFVGFTIPGLNKIPVPLLSDIPVIGPVL"
BASE COUNT         2096 a         2577 c         2431 g         1978 t
ORIGIN      
        1 aaaaccatcg gaatgatcgg cttgctgatc tgctccattg gaagcccggt aatggctcct
       61 gcaacgtaga tatttatcgc gacagggggg gtagccattc cgatggcaag ccctatggcg
      121 atgagcacgc cgaagtggag aaggtttccc ccggtgcgga tgatcgtcgg taagaagatg
      181 ggtgtgagga cgatcagtgc ggaagcagtc tccataaaca tcccggcaag aagaatgatg
      241 atcgtgatca taaagtagat catgaaggta ttcccgccgg caaaaccgag gaggaaacgc
      301 gcgaccgcct ccggtatctg aaagaaggcg agcccccatc caaagagctt cgcggtggcg
      361 atgatgaaca tgatgagcgc cgaggtgacg cccgaaccga caacgatctt gtagaactgc
      421 ttgaagctca gcgatctgta gatgaaaaat gcgacgaaga gcgagtagac caccgccacg
      481 gcagcagcct ccgaaggggt gaagaacccc gagaagatgc ctccgagaat gatgacgggc
      541 gtcataagac cccatgtggc gtcaaggaag agtttccact tctctcggcc cgtggtgctt
      601 ctgctcgcgg ggtatcctct ctttctcgcg acgtagatcg aatagacgat aagtccgagc
      661 cccatcagaa aacccgggac gaatcccgcc atgaagagct tcgccacgga aactccggcg
      721 ataactgcat agagcaccat cgggacgctt ggcggaatga ccacgccgat cgtcccggat
      781 gctgcgatca gggcggccga gaagtccaca tcgtacccct tctcctcaag ctgcgggagg
      841 agcgcggagc cgacggccgc cgtcgtagcc gctccggagc ctgagatggc cgcgatgaac
      901 atggaggcca cagtagagac gatggagagt ccgcctcgga accttccgag agtcgcctcg
      961 gcaaaagcga taatccgctc cgagatgccg cccttcgcga gaatatctcc tgcaagaacg
     1021 aaaaaaggga ccgcgacgag cgcaaacgat tcattcccgg agaacatggt ctgcggaatc
     1081 atctcaagag ggaatccgtt caaaaagacg ataagcagac ccgatacgcc gatggcaagt
     1141 cccaccggaa cccccaggag taaaaaaaac gcaaaactgc ctgcaagaac gagtcccata
     1201 ttacttcctc ccgagaatgc cgccgagtcc agcgacaaga tgcagcagaa cgatactccc
     1261 cacgaccggg tacatcagat aaatccatgt catggaaata cccatggctg gagttgtctg
     1321 catcgcctcg gacttcataa gaacgccgcc gtagtacgcg acgacgaaga agaagagaat
     1381 ccctatcccc tgcacaagga attccgccac ttttctcagc ggaagaggga gcttgtgtac
     1441 aagaaacgtt accgctatgt gagagcctcg tttgaaagct atcgcggcgc ccaggagcga
     1501 cgcggcgacc agaagatatg tcgtcagttc ctccgaccag atcagcgcct cgaagaagac
     1561 cctgcagaca atctgcattg tcgtcacggc aatcatcgcg agcatcatcg cgaagagaag
     1621 tatttcgctg atcttattaa caaaatcgct taatcggaca agaggcgaga atacgctccc
     1681 gcctcttgtc ccgttatctc tggaattcat ccgctacagc cttacttgtc agccagaata
     1741 gcctcgataa gctctttacc gaactgctct tcgtactttt ggtacacagg tttgacagcc
     1801 tcgcggaacg cggccgtgtc ggtctctgta acttccattc ccttgtcctt caggaactgg
     1861 agccactcgg cctctttctc attgtcgtag gctctgttat gctccgctgc cttctgcgcc
     1921 gcatcctgaa caagggactg catctcgggg gcaagttttt cccacgtctt tttgctcatc
     1981 ataatcacgt tcggcgcgta ggcatggcgg gtgatcgcaa gatgtttctg ggactcgaaa
     2041 agattgaagg caacgatgac gttcagcgga ttctcctgtc cgtcgatagc tccctgctgg
     2101 agtgcggtaa gcgcctcggt ccacgccata gggatcgcgt tcgcgccaag cgctctgaag
     2161 gagtcgacat agaccgggtt ctgcatgagc cggatcttca gccccttcat atcctcgggg
     2221 gtcttgacag gctgtttgct gttcgtcaga ttacggaagc cgcgctcgcc gtacgccagc
     2281 cccttccagc cgtgctgctc catctcattg agcatcattt tgccgatctc gccgtcaagc
     2341 actttgtacg catgctccgg tgtcgagaag aggaatggca gatcgaaaac accgaacttc
     2401 ggcatgaagt tcatgatcgg accgccggtg atgatggcgg aatcaaccac gcccatgcgc
     2461 atgctttcga ggagattccg ctcgtcgccg agctgcgcgt tggggtatat cgtcaccgtg
     2521 agctcccctt tgcttcccgc ctcgacaagc tccttgaact tcagcgcgca gacatggaac
     2581 gcgtcattct cattcacaac gtgcgcaagc ttgatatcca ccgccgcgac cgcggcggaa
     2641 gcgacaaaga gcaaggtcaa aaggacgatc gatacaactg cgatacttct cttcatgata
     2701 ctatcccctc ctggttgtat tgaagatatg attgtgaaaa aaccgctcct cggacacagc
     2761 atccaaagag gagcattaag ccttggcaac gcattatagc tattagcgcg agaattgcaa
     2821 gtctgtttca atgttttgcg tccagtaaac cgaatgttca tttaaggaaa cccgctacag
     2881 tcttcgccgc acatatgttt gtgattctgt ttgtgattct ggacaacgct tcgcttgcct
     2941 gctatcatcg gaacagaccg gcgttcctct ggcacattga ggacaatacg taaggagagt
     3001 ggttatacaa tggccgattt tgtgctgctc gcagggaaaa tttccgacat ccggctggcg
     3061 gaggacccat cgcatgcatc gggtatcgag aaacgaattg tcttcgcccc cgggaaattc
     3121 tggaacgact acgttgcgcg ccactttatc gtaaagggca aaaactcgaa aacgcctttc
     3181 cacacgcacg actggcccca ttacgtgttt attttatcgg gaagatgcaa cgccacaatt
     3241 gacggcacgg tctatccgct tgaaaaaggg tgctgggctc atgttcctcc gaatgtggaa
     3301 cactttttcg agagtacagg ggaagagccg ttggacttta tctgcattgt gcctcccaag
     3361 ggcgaccccg caggaagcgc ccacctcgcc tgattttcgg cgggaggcag cctttttaca
     3421 gcatccccca aagcagagag aggccggaag cgtcgcttgc cggcctctct ctttttaatg
     3481 cggaaaggaa aagttcatca tcggaggaag ccacgggtac accgaccgaa gaaagtacgg
     3541 gttcatagca aaaatgacaa acaaccccat cgccattgcg gaatggaaca ctgaaagcaa
     3601 ccccctgtat ttctccttct tggcgacagc gccaagaaac ccttcgcaga caaggaacgc
     3661 cgttatgtaa tacagccaac cgtttgttcc cggttcgtga agaaaaatca tcgtcctcag
     3721 tacgcagtag gcggaccaaa tacccagaat atacatctgg cttcctgtca ttgccagctt
     3781 cgcaataaag gcgattgctc tcggaaaaag ctcgccgctc ttttccatag cttcaagcgt
     3841 atcgaaagcc gattcgatga agggaaggat ataacgaata tatccgacgg agagaatcca
     3901 tccccaaaaa acaagaaaaa aaacataatt aagataggtc aacaaaatcc cgccttccgg
     3961 agcgtatgat aactaaagcg ctcgcaatat actgcttccc tgtactctgc gagacacgaa
     4021 atgaaacggc gcaccctcca acccgcctct tttttccaga gcgtttgttc aggcggccgt
     4081 gagcaaggca ggctacctct gttccactat cggtttcgcg taggcggttt ccgcatccca
     4141 cggaaagagg acccacgtgt cctgactgac ttccgttata taggtatcca cgaacgcacg
     4201 gccgtccggt tttgcgtaca cagcggcaaa gtgggctttc gggagcattc gtcgaacaac
     4261 ctgggcggtt tttcccgtat cgacgagatc gtcgatgata agccaccctg ccccgtcgcc
     4321 ggagatttcc tttaaaaccg aaggctcccc ctgtgtgcgc agcacgtagc tcgatacaca
     4381 gactgtatca acaagccgaa catcaagctc ccgggcgatg attgctgcgg ggatcagtcc
     4441 gcccctcgca attcctatga ttctttccca cttgcacaca ctgacaagac gccacgccaa
     4501 ggctttgcaa tcgcgatgca cctgctccca tgaaaccgga taggtcttat gatatctttc
     4561 acttgacacg ccacccactc ctccttcaat tcttctcgcg cagccgactg gtgtattgta
     4621 gcatgcccgt ttccttctga aaaagtaaaa tgcttcctct acattttttt tcttaagacg
     4681 ctactgtata atatctaagg aggggcgtac ccctcaattc ttatattcag ggaggttgaa
     4741 agcgtgaaaa gattcgttgt gctcctcgtt tttgttttga gcgtcactct gtgcggtgcg
     4801 gcgtacgccg cgttccccgc gatccccgcc gagaagatca acccgggatt tgtctacata
     4861 ggccccatcg gcgacggcgg atggacctac atgcatgagc agggccgcct tgccatggag
     4921 aaggaattcc ccggggtgaa gtcgaccttt gtcgaatcca ttccggaagg ccccgacgcg
     4981 gccagagtct tcgagaccat gatacgcaac ggctcgaagg tcattttcgg cacgactttc
     5041 ggctacatgg atttcgtcca ggaggtcgcg aagaagtacc ctgacaccat tttcatgcac
     5101 tgcgcgggat acaaagacgc cccgaacgtc ggaacatatt tcggcagaat gtaccaggct
     5161 cgttatctct ccggcctcgt cgcagggaaa atgaccaaga aaaaccgtat cggctacgtc
     5221 ggcgcacacc ccatccccga ggtcatcagg ggaatcaacg cgttcacgca gggcgtccgc
     5281 aaggcgaacc ccgaggccga agttcaggta atctggattt tctcctggct tgacccgggc
     5341 aaggaaaagg aagccgcaaa agcgctcatc gactccggag tcgatatcct tgcaatgcac
     5401 gcagataccg gcgcgacccc gcaggcggct gaagaggcgg gtatctatgt catcggatat
     5461 aataacgaca tgagcgccta tgcgccgacg aagcacctca ccgcgcccat atgggattgg
     5521 ggaatggtgt acaaggagac gatcggccag gtcatcaagg ggacatggca gcccagccac
     5581 atctggtacg gactgaaaga gggcatggtg gctctcgctc cttacggaaa ggatgtaccc
     5641 gacttggtga aagctctcgt cgacgtcgag aaagccaaga ttgtgtcggg agagtgggat
     5701 gtattccacg gcccggtgaa ggatcagagc ggcgaagtga aggttcctga aggcgctgca
     5761 atgaccgacg gagagatgct ttccatgaac tggtttgtag aaggcgtcaa gggagatata
     5821 cccaaatagc cctttctttt gtatcatata tgggggaact tcggggcgcg aatttccgcg
     5881 cccctttttc ttcccgattc atcactacat taccgatcca gaacggatac acatcctctc
     5941 cacagacact cttttgagcg gagaatccgt acactggatc gcaaggggga aaagggatgg
     6001 atgcacgagc tccttcgaaa ccgctcgtcc gcatggaaaa aatcaccaaa cgctttacag
     6061 gggtgacggc caatctcgac gtggacttcg acgtccgccc gggagaagtg catgcccttc
     6121 tcggggagaa cggcgcgggc aaatcgaccc tgatgaatat tctctacggc ctctataggc
     6181 cggacagcgg cgtcctgact atagaaggga aggacgtttc tttttcttcc ccgcgggatg
     6241 ccatcgcctc gggaataggg atggttcacc agcacttcat gctcatcccg agccagacgg
     6301 tatgggagaa catgatactc ggtcttgaag gactctcttc ggtcctccct aaacgcgcga
     6361 ttcgggagcg tatcctcgaa ctctcctccc ggtatggact gtcggttgat cccgacgcga
     6421 aaatctggca gctctccatc ggtgaacagc agcgtgtggc gattctccag atgctctaca
     6481 ggagggcaaa aatccttgtg ctcgacgagc ccacggcggt gctcacacct caagaggcgg
     6541 agcatctctt cgtgactgta cgccagatga catccgaagg acacggcatc gtcttcatct
     6601 ctcacaaaat ggatgaggtc atggggcttt cccatcgggt gacgatccta cggaaaggcc
     6661 gccttgtagg aactgtcgat acggcgtcca caacaaagga aaagctggcg gagatgatga
     6721 tcggccagaa gatgatcttc agtattgaga agaaagagct tcagcccggg aaaccggtta
     6781 ttgaatgcgc ttccgtctgc gccctcggcg accggggcat tcccgccgta tcgcgcgcga
     6841 cgttcatgat ccgggagcag gaaatcctgg gcgtcgcagg agtggcggga aacggccagc
     6901 aggagctttg cgaaacactg gtgggacttc gaaaggttga gtccggtgca attcgagtca
     6961 ataacgagga gctgaccaat tcgattccga agaccttcat cgacaaaggg atacactata
     7021 tccccgccga ccggaaagga accgggctcg ttcccaatat gaatgtgagc gagaactcca
     7081 tcctgaagag atactggagc cgcgccgttt ccaagaaggg attcatcgac tggaaagaag
     7141 tcttctcttt cgccagaggg ctggtttccc gctacaatgt gagcactcct tcgatggaaa
     7201 ctcctgttcg caacctctcc ggagggaacc tccagaagct gatgctcgga cgcgaactga
     7261 gcgactcccc gatagccatc ctcgccgttc atcccacatg gggactcgac gtcgcggcga
     7321 cgaagttcgt cagggaacag cttctcgccg cccgcgaggc tggatgcgcc gttctgctgg
     7381 tttcggagga tctcgacgaa ctcctcgcca tgagcgaccg ccttgccgtt atgtgcaagg
     7441 gcgagatcat gggcattctg gattcgcctg cggagacgcc tgtcgaagcc atcggcctta
     7501 tgatggccgg aacacccgct gaagaggcgc ttgcctccgc tccccgagga ggagaaaaat
     7561 catgaaactc tctatcaaag tggaaccccg acttgaaacc ccgcggatgc tcggcattgc
     7621 cattcccgtc atctcactcg cagccgcgct tctttcggga gctgtttttc tcttcttcat
     7681 gggcgtctct ccgttccagg cctactccac catcatccgc tcttctttgg gtgacgcgta
     7741 cggtctcagc gagactctcg tcaaggcgat ccctcttgca atggctgcga tcgcggtgat
     7801 gctctccttc acaatgctca tttggaatat cggcgcggag gggcagatct tcatgggagc
     7861 tattgccgcc gccgccgctg ttcgctacct cttcgtcgac aaccacctgg cgatgttcgc
     7921 catcatgttt gcggcagcgg gaatcgcagg gggaatctgg gcggccatcg ccggatactt
     7981 ccgcgcgcga tggagcgtca acgagatcat cacgacgctg atgctcaatt atgtggccat
     8041 gaacctgctg aactattttg tctacggccc gtggcgcgac cctgcaagcc tcggcttccc
     8101 gatgacggcc ccttttccgg acgcggctcg tctcccctct ttctggggca cccgaatcca
     8161 tgcagggctt ttcctcgcaa tcctgtttcc tctgatcttc tgggtgattc tccgtttcac
     8221 caggtggggg tacgagattc gggtcatggg agaaaatccc aaagctgccg gttttgcagg
     8281 gatgagctat attaaaaacg tggtgttcgt gatgtttctt tccggagcta tcgccggaat
     8341 agcggggatg tgcgaactct ccgggctgca gggccgctta cagcacggtt tttccggcgg
     8401 ctacggctac accgcgatta tcgtggcatg gctggcccgc ctgcacccga tcgcaatcat
     8461 actggtctcc ttcctcatgg gggtgctcct ggtaggcggc gagactcttc agatcgtcat
     8521 gcggcttccc ctttcaagca tgctcgtgct gcaaggtttg atcctcttct ttgttctcgg
     8581 cggtgagttt ttcaggaaat tccgtatccg cattctctcc ggaggcgcga ctcctccgat
     8641 cgcaacgacc tctgcaaagg aggagaagta gcatggaaat tctgattccc attctcacag
     8701 cggctgtccg aagcggcacg cctattctgt acgcgactct cggcgagatc gtcacggaaa
     8761 aaggaggcat cctcaacctc ggacttgaag gcatcatgct cctgggcgcg tatgcgggat
     8821 tcgcagtctc ctactccacg gggaacgcat ggcttgggat cgctgcggca ttcttcgttg
     8881 gaacgctcct ggggcttgtt cacgctttca tctgcgtcac gctgaaaggc aaccaggtag
     8941 tcagcggtct cgccatgacc atgttcggta cgggcgcgtc ctctctcctg ggccgcaatt
     9001 tcgtcggctt caccatcccg gggttgaaca agattcccgt tccgttgctc tccgacatcc
     9061 ctgtcatcgg acctgtgctc tt
//
LOCUS       DAOPKU010000040         8950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8235_length_8950_cov_5.101405, whole genome shotgun sequence.
ACCESSION   DAOPKU010000040 DAOPKU010000000
VERSION     DAOPKU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8235_length_8950_cov_5.101405"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PK849_02200"
     CDS             complement(<1..363)
                     /locus_tag="PK849_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPE64964.1"
                     /translation="MRNIFDEIEELYEKTRRKERNFDGEEYGGGGGNEKPRRRFSFSF
                     GFLKLLLPVLLLLIVAWQGFYIVPAGSRGVVFRLGKISHVANQGMNFKLPVVDQVTIV
                     NTEQIQRFEYGYRTVNPGP"
     gene            complement(360..1220)
                     /locus_tag="PK849_02205"
     CDS             complement(360..1220)
                     /locus_tag="PK849_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HPE64965.1"
                     /translation="MSGWWYNDYTPAPPRAVRGGIKASGKGGFAKKWWGKRWLDVLES
                     FGIGARLSRGRAYAKKGQVADLSIGTGLIRASVQGTRVKPYTITIQLAPYSAAQWDEI
                     FGAIAEHPVVAARLLAGEMPEEMEELCAKRKLPLFPKRQGDMVTECSCPDWSNPCKHI
                     AAVFFVMAEAFDGDPFLLLRLRGMDRDVFISRIAPGASEEEHEEEIGAEPLPAEPSRF
                     WGPEGEIPEIRIPASLPKVHGALPGRLGPLPFWRGKETFPETMAALYESATCRAEEIY
                     RTLEENGGIR"
     gene            complement(1217..4252)
                     /locus_tag="PK849_02210"
     CDS             complement(1217..4252)
                     /locus_tag="PK849_02210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPE64966.1"
                     /translation="MKALHISLLNGNPVVWCEGGSLFDALEAIGFPLPRSAPLSAMTL
                     WLPGRKSVPFPSSSLLGEAEGRGKVALVPFRTQGRSPSTFAEAAALAYRFEGSSPRGS
                     GVLFGSSVRWFAAAMRAVRLLTAGESVLPAAARTEGVWEARWIPAPDDSAQRLLEELA
                     ASMPHVCRSIASDEQGPPDHSPRRVLAAFLGRFVDLAMRGEERRKEEAPKGRKKKTAS
                     SDAGSLHDAWREALSGADARILWSDENELSEFARALSAWRRPVEFASKAAYRFCFRLE
                     EPEEGDDWRLVYLVQPKADPSLLLPLDALWEQTPPPKKSRKKGPAPSPLPAVRDVSAE
                     FLLTMLGQAAALSPRVAQSLKQPAPSSIRMSSEEAFSFLGGEAQTLADAGFQMMLPRW
                     FVKGPGERIKVKAVVKAPPMQSASGMSLESVMEFDYALSLAGETLAPEELEMLVMLKG
                     DFVRFRGQWMKVDRESIASGLRLFSQKKETRATGRDLLMKAVGGEGALSGVSVDSAEL
                     HGWPAELLAKLVEKRSDDLPSPEGLRGVLRPYQQRGYSWLAFLKEWGLGACLADDMGL
                     GKSVQALALVQRERERGETRPALLVCPTSVMDNWRREAEKFTPGLSVAVHWGASRARR
                     GDFAGVAAANALVVSSYALVQRDIAFLKEVEWAGVLLDEAQNVKNPEAKQSRAVRSLR
                     SGYRVALTGTPVENHVGDLWSLMDFLNPGLLGSASAFKRRFHTPITRDGDTEAAERLR
                     RATAPFILRRLKSDRSIISDLPDKIETKSYCPLTKEQASLYAAVLADIDERLTNSEGI
                     ERKGLVLAALTRLKQVCNHPAHFAGDGSALTGRSGKLERLMEMLEEARESGGRTLVFT
                     QFAEMGELMASHIREYFAEEVFLLHGGVPKKKRDEMVARFQEDAAAPHVFILSLKAGG
                     SGINLTRADRVIHYDRWWNPAVENQATDRAYRIGQTKNVQVYKFLVAGTLEEKIDALI
                     ERKSAIAGTIIGAGENWLTELSDGELRDLLALEKEAVVE"
     gene            complement(4266..4682)
                     /locus_tag="PK849_02215"
     CDS             complement(4266..4682)
                     /locus_tag="PK849_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPE64967.1"
                     /translation="MPTVDDVCRNKGSIIERTLRRMKEEYAADPELKSFTHIDAMTLN
                     IERACQAAIDAAMHIAARDHLGMPRNSADVFRLLHRAGRLSETTARAMVAMTGFRNVA
                     IHEYRELDMSVLRAIAADRWTSLVDFCREIGLTIRP"
     gene            complement(4669..5106)
                     /locus_tag="PK849_02220"
     CDS             complement(4669..5106)
                     /locus_tag="PK849_02220"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPE64968.1"
                     /translation="MTDFFETLSPAEKDTGGSIPEETVRLIAERCECDERVLAVYLLG
                     SAARGTMRPDSDIDIGVLPAPGEFFSALELAEISAEISFETGYVVDAGLVDGSDLVYA
                     NQALDGVRLFVREGTTTELREAALRAMYLRFCEDRKEVVDAYS"
     gene            5237..5566
                     /locus_tag="PK849_02225"
     CDS             5237..5566
                     /locus_tag="PK849_02225"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPE64969.1"
                     /translation="MNTVRGIFAPIATAFDASGEVNYDVFAENVRSFGATKLAGLVVL
                     GSNGEFTLLSHDEKVKLVETARKHLPSDKKIIAGTGCESLKETVELTKQCAAAGADVA
                     LVVTPNY"
     gene            5574..6128
                     /locus_tag="PK849_02230"
     CDS             5574..6128
                     /locus_tag="PK849_02230"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPE64970.1"
                     /translation="MNEAALEKFYTMLADASPIPVMIYNMPGNSGVNVPSPLTLKLAA
                     HPNITGIKDSGGNIVQISEVLAKAPSDFSVFAGSGSYLLATLVLGGVGGTLAVANVVP
                     DYCAEIQERFEKGDLDGARKMQLALLPLNAAVTSRFGIGGMKAAMDMVGYKGGLPRLP
                     IQPASEETKREIARILKELGVPTV"
     gene            6131..7321
                     /locus_tag="PK849_02235"
     CDS             6131..7321
                     /locus_tag="PK849_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HPE64971.1"
                     /translation="MLVSGDRFLADLEALGLIGWVEGEGMRRPAYSASYDEARRFVAA
                     KMKEAGLTVRVDGVGNLFGRLEGSDPSLPSIMSGSHLDAVPGGGKYDGPLGVMAALEA
                     ARSIKERKLPLRHPFEAVGFTAEEGGEMGGTFGSRAMAGLLDEPLPEEKLASVGLTPE
                     MVRSSKRDPSRIACYIELHIEQGPFLERRGISIGIPTGIVGIGRYAVRLTGEANHAGT
                     TPMKERRDAMREAAELLSEWFAWTDARDDMVCNVGVFSIHPGAAAVVPDRAEFTLEIR
                     SLKDSVMEEAASAFRRLLEKRTRVAVSMEPLIWKGAVELSPLLQEAAEKACGEMGVSC
                     VRMPSGASHDAAPMAGIVPAGMLFVPSAGGISHAKEEATPPDDLVRGAEALLRTVLEA
                     DARL"
     gene            7502..8548
                     /locus_tag="PK849_02240"
     CDS             7502..8548
                     /locus_tag="PK849_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPE64972.1"
                     /translation="MEKFQVMIGLVGVIALVAVGFVLNFAQAVFIPLIIAWLLSYLFG
                     PIVRYLAKRRVPVGLAVVFVLAIFFGLCFLGAIFLNTRIASFAAAFPKYYSRLMEIGK
                     SLTTNLDIPPDFWTSINWGFTIRGYLLDLSSSFVTIISKLVMVIVFLVFMLLGSPYFE
                     YKVKKAFVTPHGSERARKILTTISWQIGRYLSALALISAVTGLIVWMSLEYLQVDFAV
                     TWGVLAFILNFIPTIGSIVASIPPILVAVVQFYPSLTAPLITALTLLTIQVVIGNFIT
                     PKVMGDRLNLSPVVVLISLLFWGLIWGVVGALISVIIAAIIKIVCENFPMLNVISVMM
                     GSGRVYQKEFEEKR"
     gene            complement(8600..>8950)
                     /locus_tag="PK849_02245"
     CDS             complement(8600..>8950)
                     /locus_tag="PK849_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPE64973.1"
                     /translation="GHTVGHALEATSGYRWRHGDGVAAGMMVAGRLAHRLGEFRKEEL
                     ARLERLLEAFRIPCRPDMPWDEIAPFLARDKKFTGGAPRLVLPRTGERCRLRDDVTLA
                     ALRDAYEEELLKAR"
BASE COUNT         1584 a         2857 c         2750 g         1759 t
ORIGIN      
        1 aggtccgggg ttcaccgtcc ggtagccgta ctcgaagcgc tggatctgtt ccgtgttcac
       61 tatagtgacc tggtcgacga cgggaagttt gaagttcatt ccctggttcg ccacgtggga
      121 gatctttcca aggcggaaga cgacgccccg cgaccctgcc gggacgatat agaacccctg
      181 ccaagcgacg ataagcagaa gcagtacggg gagcagaagt ttcaggaagc cgaagctgaa
      241 ggagaaacgg cgccggggtt tttcgttccc cccgcctccc ccgtactcct cgccgtcgaa
      301 gtttctttcc ttgcgtctcg tcttttcgta aagctcctcg atttcatcga aaatatttct
      361 catctgatac ctccgttttc ttcaagtgtg cgatagatct cttccgccct gcacgtcgct
      421 gattcgtaga gagccgccat cgtctccggg aaggtctcct tacctcgcca gaaggggagc
      481 ggtccaaggc gtccggggag cgccccgtgc actttcggga gcgatgcggg aatcctgatt
      541 tcgggaatct cgccttccgg cccccagaag cgcgacggct ccgccggaag tggttcggct
      601 ccgatctcct cctcgtgttc ctcctccgat gcgcccgggg cgatgcggga gataaagacg
      661 tcccgatcca ttccgcgcag cctgagcagc agaaagggat ctccgtcgaa cgcctccgcc
      721 atcacgaaaa agactgcggc tatgtgcttg cacgggttgg accagtcggg gcaggagcat
      781 tcggtgacca tgtccccctg tcgtttcggg aaaagaggaa gctttctctt cgcacacagc
      841 tcctccatct cttcgggcat ctccccggcc aaaagacggg ccgcgacgac cggatgttcc
      901 gcgattgcgc cgaagatctc gtcccactgc gccgcggagt agggggcaag ttggatagtg
      961 atcgtatacg gtttgaccct cgtcccctgg acggatgccc gtatcagccc cgtcccgatg
     1021 gagaggtcgg ccacctgccc cttcttcgcg taggcgcgtc cacgcgagag acgggcgccg
     1081 atgccgaagg attcgagcac atcgagccac cgttttcccc accacttctt cgcgaagccg
     1141 cccttgcccg aagccttgat tccgccgcgc accgcccgcg gcggcgcggg cgtgtagtcg
     1201 ttgtaccacc atccgctcat tccaccacag cctccttctc cagtgcgagc aggtcgcgca
     1261 gctctccgtc cgagagttcc gtcagccagt tctcccccgc gcctataatt gtacccgcaa
     1321 tcgcggattt gcgttcgatg agcgcgtcga ttttctcctc cagcgtcccg gcgaccagaa
     1381 atttgtacac ctgcacgttt ttcgtctgcc cgatccggta ggctctgtcg gtagcctggt
     1441 tctccacggc cggattccac caccggtcgt agtggatcac cctgtccgcc cgcgtcaggt
     1501 tgatccccga accgcccgcc ttcagagaga ggatgaagac gtgcggggcg gcggcgtcct
     1561 cctgaaagcg cgcgaccatc tcgtcgcgct tctttttcgg cacgccgccg tgaagcagga
     1621 aaacctcttc ggcgaagtac tcccggatat gcgacgccat caattcgccc atttccgcga
     1681 actgggtgaa gacgagcgtg cgtccgccgc tctccctcgc ttcctccagc atctccatca
     1741 gccgctccaa cttgccggaa cgccccgtca gcgccgatcc gtccccggcg aagtgcgccg
     1801 gatggttgca cacctgcttc agccgtgtca gcgccgcgag gacgagcccc tttctttcga
     1861 tgccctctga attggtcaga cgctcgtcga tgtccgcgag gaccgccgca tacagagacg
     1921 cctgctcctt ggtgagcggg cagtagctct tcgtctcgat cttgtccggc aggtcggaga
     1981 tgatgctcct gtccgacttc agtctgcgga ggataaaggg ggccgtcgcg cggcgcaggc
     2041 gttccgccgc ctccgtgtcg ccgtctctgg tgatgggcgt gtggaagcgc ctcttgaacg
     2101 cgcttgccga gccgaggagc cccgggttca ggaagtccat cagggaccag agatcgccga
     2161 cgtggttctc cacgggcgtt ccggtcaagg caacgcgata ccccgagcgg agagagcgca
     2221 ccgcgcggga ctgcttcgcc tcggggttct tcacgttctg cgcctcgtcg aggagcactc
     2281 ccgcccactc cacctctttg aggaatgcga tgtcccgctg gacgagggcg tagcttgaaa
     2341 cgaccagcgc gttcgccgcg gcgacgcccg cgaagtcgcc gcggcgcgcg cgcgaagcgc
     2401 cccagtggac cgcgacggag agtccgggcg taaacttctc cgcctctctc ctccagttgt
     2461 ccatcaccga ggtggggcag acgaggagcg ccggacgggt ctctccccgt tcgcgctccc
     2521 tctggacgag ggccagggct tgtacgcttt tcccgagccc catatcgtcg gcgagacagg
     2581 cgccgagccc ccactccttc aggaaggcga gccacgagta cccccgttgc tggtaggggc
     2641 gaaggacgcc gcgtagtcct tccggagagg ggagatcgtc gctcctcttc tctacgagct
     2701 tcgcgagcag ctccgcaggc cagccgtgca gctccgcgga atcaacggag acgccggaga
     2761 gcgccccctc gccgcccacc gccttcatga gcagatcgcg gccggtagcc cgcgtttctt
     2821 tcttttgaga aaagagacgg agcccggaag caatgctctc gcggtccacc ttcatccatt
     2881 gaccgcggaa acggacgaaa tctcccttga gcattacgag catctccagc tcttcgggcg
     2941 cgagcgtctc gccggcgagc gagagcgcgt agtcgaactc catcacgctt tcgagcgaca
     3001 tccccgatgc gctctgcatg gggggcgctt tcaccaccgc ctttaccttg atgcgttctc
     3061 cggggccttt tacgaaccat ctgggaagca tcatctggaa ccccgcgtcg gcaagcgttt
     3121 gcgcctcccc tccgaggaag gaaaacgcct cctcggagga catgcggatc gaagagggag
     3181 cgggttgctt caggctctgc gcgacccgag gagaaagagc cgcagcctgt ccgagcatcg
     3241 tgaggagaaa ctccgcagag acgtctcgga ccgcgggaag cggcgaggga gcgggccctt
     3301 tcttgcggct ttttttcgga ggcggagtct gctcccagag cgcatcgaga ggaagaagaa
     3361 ggctcgggtc ggccttcggc tggaccaggt agacgagccg ccagtcgtcg ccctcttcgg
     3421 gctcttcgag gcggaaacag aagcggtacg cggccttcga ggcgaattcc acagggcggc
     3481 gccatgccga aagcgcgcgt gcgaactccg agagttcgtt ttcatcggac cagaggatcc
     3541 gggcatccgc tccggaaaga gcctctctcc acgcgtcgtg gaggcttccg gcgtccgacg
     3601 acgctgtttt cttctttctg cccttcggcg cctcttcctt ccgtctttct tcgccgcgca
     3661 tcgccagatc gacgaagcgc cccagaaagg ccgccagaac gcgccgggga gagtggtcgg
     3721 gcgggccttg ctcatcggaa gcgatgctcc ggcagacatg gggcatcgag gccgccagct
     3781 cttcgagcag acgctgggcg gagtcgtcgg gcgccggaat ccagcgggct tcccatacgc
     3841 cctccgtccg tgccgccgca gggaggacgg attctccggc cgtgagcagc cggacggccc
     3901 gcatcgccgc ggcgaaccac ctgacggacg agccgaagag aacgcctgag cctcgcggag
     3961 agcttccctc gaaccggtac gcgagcgcag ccgcctccgc gaacgtagac ggagaccgcc
     4021 cctgcgtacg gaaggggacg agcgcgacct ttccgcgccc ttccgcctct cccagcagag
     4081 atgacgaggg gaacgggacg ctctttctcc ccggcagcca aagagtcatg gcgctcaggg
     4141 gggccgagcg gggaagggga aatccgatgg cttcaagcgc gtcgaaaaga gatccacctt
     4201 cgcaccagac gacagggttt ccgttcaaca gcgagatgtg cagtgctttc aaggggttca
     4261 gctcctcaag ggcggatggt caggccgatc tcccggcaga agtccacaag ggacgtccac
     4321 cggtcggccg cgatggcgcg aagcaccgac atatcaagtt cgcggtattc atggattgcg
     4381 acgttgcgga accccgtcat cgcaaccatc gcccgcgccg tcgtctcgga gaggcgtccg
     4441 gcccgatgga gcagacggaa gacgtccgcg ctgttgcgtg gcatgccgag gtgatcccgc
     4501 gccgcaatgt gcatcgccgc gtcgatggcg gcttggcagg cgcgttctat gttcagcgtc
     4561 atggcgtcga tgtgcgtgaa gctcttcagc tccgggtcgg cggcatattc ttccttcatc
     4621 cggcgcagcg tccgctcgat gatggagccc ttgttcctgc atacatcgtc aactgtaggc
     4681 atcgacgacc tcctttctgt cctcgcaaaa gcgaaggtac atggcgcgca gcgccgcttc
     4741 gcgcagttcc gtcgtcgtcc cttcccggac gaaaagacgc accccgtcca gcgcttggtt
     4801 ggcgtagaca agatcgctcc cgtcgacgag cccggcgtcg acaacgtatc ccgtttcgaa
     4861 ggagatttcg gcggagattt cggcgagttc aagagcggaa aagaactctc cgggggcggg
     4921 aagaactccg atgtcgatat cgctgtcggg gcgcatcgtc ccccgagcgg cgctgccgag
     4981 cagatagacc gcaagcacgc gctcgtcgca ttcgcaacgc tccgctatca accggaccgt
     5041 ctcttcgggg atcgagcccc ctgtatcttt ctccgccgga gaaagagttt caaaaaagtc
     5101 cgtcataccg cttccccctt ttgcttcacc aacattgtaa cgcatcggcc cgcacaaaat
     5161 cgagagcctc ttcacggaaa cggttttttg aagtaccata taagaaacat catgcattct
     5221 aaaggagaga tcgggtatga atacggttcg cggaattttc gcccccatcg ctacggcgtt
     5281 cgacgcttcg ggagaggtga actacgacgt tttcgcggag aacgtgcgct ctttcggcgc
     5341 gacgaagctt gcggggcttg tggttctggg gagcaacggc gagtttacgc tgctctcgca
     5401 cgacgaaaag gtgaagctgg tggagacggc tcgaaaacac ctcccctccg acaagaagat
     5461 catcgccgga acggggtgcg agtcgctgaa ggagaccgtc gagctgacga aacagtgtgc
     5521 ggccgcgggc gcggatgtgg cgctcgtcgt caccccgaac tactaaaaaa gatatgaacg
     5581 aggccgcgct ggagaagttc tacacgatgc tcgccgacgc ctccccgatc cccgtgatga
     5641 tctacaacat gcccggcaac tccggcgtga acgtcccctc cccgctgacg ctcaagcttg
     5701 ccgctcatcc gaacatcacc ggcatcaagg acagcggcgg caacatcgtg cagatctccg
     5761 aggtgctggc caaggctccc tcggactttt cggtcttcgc cgggtcgggg agctaccttc
     5821 tggccaccct cgttctcgga ggcgtcggcg gcacccttgc cgtggccaac gtcgtccccg
     5881 actactgcgc cgagattcag gaacgcttcg agaagggcga cctcgacgga gcgcggaaga
     5941 tgcagctcgc cctgctgccg ctgaacgccg cggtcaccag ccggttcggc atcggcggca
     6001 tgaaggccgc gatggacatg gtcggctaca agggaggcct cccgcgcctg ccgattcagc
     6061 ccgcgagtga ggagacgaag agggagatcg cccggatact gaaggagctg ggcgttccta
     6121 cggtgtagcc atgctcgtct ccggggatcg ttttctcgcc gatcttgagg cgcttgggct
     6181 cattggctgg gtcgaggggg agggaatgcg ccgtccggca tattccgctt cttacgatga
     6241 ggcgcgtcgg tttgttgcgg cgaagatgaa agaggctggg ttgaccgtca gagtggacgg
     6301 cgtgggaaac ctcttcggca ggctggaggg gagcgacccg tcgcttccct ccatcatgtc
     6361 cggttcccat ctcgacgcgg ttcccggagg cgggaagtac gacggccctc tcggcgtcat
     6421 ggccgcgctg gaggcggcgc gctccatcaa agaacggaag cttccgctcc gtcacccctt
     6481 cgaggcagtg ggcttcaccg ccgaggaggg aggcgagatg ggcggcacct tcggaagccg
     6541 cgcgatggca gggctgctcg acgagccgct cccggaggaa aaacttgcct ccgtagggct
     6601 caccccggaa atggtgcgct cgtcgaagag ggatccttcg cggatagcct gctacattga
     6661 gctccacatc gagcaagggc ccttccttga gcggcggggg atatccatcg gcatccctac
     6721 cggcatcgtc ggcatcggcc ggtacgccgt ccgcctgacc ggcgaggcga accacgccgg
     6781 gaccacgccg atgaaggagc gcagggacgc catgcgcgag gctgcggagc tgctgagcga
     6841 atggttcgcg tggacggacg cgcgggacga catggtctgc aacgtcggcg tcttctcgat
     6901 tcatcccggg gctgcggcgg tcgtgccgga ccgggcggag ttcacgcttg agatccgctc
     6961 gctgaaggac tcggtgatgg aggaggccgc ctctgcgttc cgcaggcttc tggagaagcg
     7021 gacccgcgtc gccgtgtcga tggaaccgct tatttggaaa ggagcggtgg aactctcccc
     7081 gctgctccag gaggcggccg aaaaggcatg cggcgagatg ggcgtctcct gcgtcagaat
     7141 gccgagcggc gcgtcgcacg acgcagcccc gatggccggc atcgtcccgg ccgggatgct
     7201 cttcgttccc agcgcgggcg gtatcagcca tgccaaagaa gaagcgaccc cgccggacga
     7261 tcttgttcga ggggcggagg cccttctgag gactgtgctt gaagctgacg cgcgcctgta
     7321 gacaggaagc ggcggactcc gccttttccc cgaatgcggc ggagtctgca gatgtgtgtt
     7381 ttccttcttt tttcagcgcg atgtattttt tattcaccct tttctcccaa aacggattcc
     7441 tcagtgtaga ataataagtg tggaggggtc tgctcgcatc tcaaggaagg gaagggatag
     7501 tgtggaaaaa tttcaggtta tgatcggcct cgtgggagtg attgctctcg tcgccgttgg
     7561 ttttgtgctg aacttcgcac aggcggtctt cattccgctt attattgcat ggcttctttc
     7621 gtatcttttt ggtcccatcg ttcgttacct cgccaaacgc cgcgttcctg tggggcttgc
     7681 ggtggttttt gtccttgcca tttttttcgg tctctgcttc ctcggcgcta tctttctgaa
     7741 tacccgaatc gcctcgttcg cggccgcctt tccaaagtac tactcgcgcc tgatggagat
     7801 aggcaagtcg ctcacgacga atctcgatat tcctcctgac ttctggacgt ccatcaactg
     7861 ggggttcacc atcagggggt atctcctcga tctctccagc tccttcgtca ccatcatctc
     7921 gaaacttgtt atggtcatcg tcttcctcgt ctttatgctg ctcggctctc cgtatttcga
     7981 atacaaggtg aagaaggcgt tcgtaacgcc tcacgggtcc gagcgagccc gcaagattct
     8041 gaccaccata tcgtggcaga tagggcgcta cctcagcgct ttggcgctca tcagcgcggt
     8101 gaccgggctg atcgtctgga tgtcccttga atacctgcag gtggatttcg ctgtaacgtg
     8161 gggagtgctc gctttcatcc tcaacttcat ccccacgatc ggctcgatcg tcgcgtccat
     8221 tcctccgatc ctcgtggccg tagtccagtt ctacccgagc ctgacagccc ccctgatcac
     8281 cgcgctcacg ctgctcacca ttcaggtggt catcggcaac ttcatcacgc cgaaggtcat
     8341 gggcgaccgg ctcaacctca gcccggtagt ggtcctcatc tcgctgctct tctggggact
     8401 catctggggc gtggtcggcg cgctcatctc ggtgatcatc gccgcgatca tcaagatcgt
     8461 ctgcgagaac ttcccgatgc tcaacgtgat cagcgtgatg atgggctcgg gaagggtcta
     8521 ccagaaggag ttcgaggaga agcgctaaac cgtccggaca cctgcggcgg aatatgaaga
     8581 gaggagctcc ccgggcgttt cagcgcgcct tgaggagctc ctcttcgtat gcgtcccgaa
     8641 gcgcggcgag cgtcacgtcg tcccgcaggc ggcagcgttc gcccgtgcgg gggagtacca
     8701 ggcggggagc gccaccggta aatttcttat cccgtgccag aaacggggcg atctcgtccc
     8761 acggcatatc cggacggcat ggaatcctga acgcctccag aagccgttcc agacgagcca
     8821 gctcctcctt ccggaattcg ccgaggcggt gcgccagcct tccggcaacc atcatccccg
     8881 cggcgacgcc gtcgccgtgc cgccatctgt agccggacgt ggcttcaagc gcgtgtccga
     8941 cggtgtgccc
//
LOCUS       DAOPKU010000041         8896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8308_length_8896_cov_4.237982, whole genome shotgun sequence.
ACCESSION   DAOPKU010000041 DAOPKU010000000
VERSION     DAOPKU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8308_length_8896_cov_4.237982"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PK849_02250"
     CDS             <1..544
                     /locus_tag="PK849_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE64974.1"
                     /translation="ILGDSTFFHSGITGLIDAVVNKSRGVFVILDNRITAMSGQQENP
                     GTGRTLMGEETTALDIPRLCAACGVKEDHIHVVDPYNLKACRDAVDVAYAAEETSVIV
                     TTRPCALIKEVQKSRASLHCVVDAEKCVLCSSCLKLGCPAITKQEGRIVIDSVACNGC
                     SLCVQVCPKQAISREGELHE"
     gene            537..1118
                     /locus_tag="PK849_02255"
     CDS             537..1118
                     /locus_tag="PK849_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPE64975.1"
                     /translation="MNKNGKNVLLVGVGGQGTILASKLLSEGLLALGYDVKMSEIHGM
                     AQRGGSVSTHVRYGARVYSPVMGAGEADVLVAFEKMEALRWLSYLSPEGMLIVNNHEI
                     YPMPVILGMSEYPARLEETLAEAATRMLLIEAADEAAALGNPRVSNVVLLGAVVKSLG
                     LEDPCWAETLRSCVPPHALQVNERAFRKGLSLV"
     gene            1391..1891
                     /locus_tag="PK849_02260"
     CDS             1391..1891
                     /locus_tag="PK849_02260"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPE64976.1"
                     /translation="MIDFTKGYSFNEYLEKGYEEERDRQRRACSRAVFSPAFEEAARN
                     VNKPLKLAAFAEIYCPDSVVTMSFVHRIAELNPKIELAVFPRSPFEKELEALTGSSRI
                     PTLLFFDEKMNLAGSYVELPKALKEDMRDLDAGEKSALTLDYRRGKYNDILQAELTEI
                     LSLFAS"
     gene            1970..2503
                     /locus_tag="PK849_02265"
     CDS             1970..2503
                     /locus_tag="PK849_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPE64977.1"
                     /translation="MPLSSIFLLFLRIGAFTFGGGLVMLGFIEKELRSTARLSSEEIA
                     DMMVLATAFPGPVAANMAFLAGKRLAGIAGSFCALLGTVIPPFLTILLLSKAVIHFMN
                     EPWLAAFFLGAACAVVVIIGRVVYSMTRISCGGRWKDIAVFAAVGILLISTDVHPFFA
                     LAGGVTVRLLLGEEDAG"
     gene            2500..3033
                     /locus_tag="PK849_02270"
     CDS             2500..3033
                     /locus_tag="PK849_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPE64978.1"
                     /translation="MSVLGTLALVFARIGLGAFGGGLATIPFIHFELVASRAWLSERG
                     FAEVVSLAQMTPGPLAVNAATFVGYRIAGVAGASVATFSVIAAPLLVLGLALFLISRA
                     SEKWKTRVDRLQRALRPAVSGMLFAAFWMVARSLTGDWRLWVMTAGVFMLIGQEYFKR
                     YPQLLLLIAGMAGVLFL"
     gene            3060..4901
                     /locus_tag="PK849_02275"
     CDS             3060..4901
                     /locus_tag="PK849_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPE64979.1"
                     /translation="MEMKIARVNLTESTIEIFSAEDELLRKYLGGSGLGTYYLFKYGS
                     PKCAALSPENPLLFMNGPFQGTGIPTSGRHQVLAKSPLTDMFGESDCGGAFGYHMRKA
                     GFDGIILLGKAPSPVYIALLDGKIEIRSASSLWGKDTFETEEFLLELEGKPAGVACIG
                     PAGENQVLLANIMHDGRNARAAGRGGMGAVMGSKNLKAIVARGSAKVLMADEKAVREK
                     SAEKAKYYMEKLPAMTRFGTAGGVALAEQNGDLPLKNWSMGSWTEDVKSITGEAMAET
                     ILTGNWGCMSCPIRCGRDVEFDGIKGAGPEYETIGMLGSNCLISDLKAIARGNDLCNR
                     MGIDTISGGSAVAFAMELYERGIISEEEIGYPLLWGDGESMIRLLSDIVEKRGFGALL
                     SEGTKKAAERIGKGAERYAIHSKGMDFPAHDPRCYKSLATGYATSNRGACHLSGFSYS
                     FERSMTYPEFGVDTVMDRTVDEGKGAVNIPFQNMMGVLDSLKMCKFPFAITRIDDILQ
                     WINGITGWNMSGDELLLAGERIFNLKRLFNVACGASALDDTLPERILTEPRGSGGSAN
                     TLPDLKLQLKEYYPERGWSEDGIPTEEKLKSLDLEEFLCCVNGPALE"
     gene            4928..6091
                     /locus_tag="PK849_02280"
     CDS             4928..6091
                     /locus_tag="PK849_02280"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HPE64980.1"
                     /translation="MVHKIVRYSVLLFILVPLLTWFASRGADMVALARWNTEDSAIVI
                     LEKVLGREDDVESGAEGNWETERVHIRITYRNGAFAGTTEEIEILQLADSRLELVPGR
                     EYLFLVDTFDDGTHQYSISDRYRIPAVAGFITLACAALAVLAGTSGVKALCGLFLSLF
                     LLLGWFVPMLAEGYTPVPFAILAIAGVSLLTIVFVVPKRALWPIPFIGAVGGAIGASV
                     TGWIMVGLWQLTGLENDSAVLLFSVAPHLELRGLLLAAVMVGGIGAVLDVAVSVTSSM
                     AELYSYDPSIPLRRLWRAGINVGSDVLGSMINTLILAYLGSSLPFVVLISMEGPDLVA
                     LLNDPHIAQEILRSVAGTVGLLLTIPVTAGAGVWWIARSHIPDEKRHNEELAD"
     gene            6160..7167
                     /gene="gap"
                     /locus_tag="PK849_02285"
     CDS             6160..7167
                     /gene="gap"
                     /locus_tag="PK849_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPE64981.1"
                     /translation="MKKIAINGLGRIGRLVLRCYMANKPADVEIVALNDLTPASEMAY
                     LIKFDSIHRKANFSVEARENSLVLDGKEIPLFKEKDPAKLPWKELGVDIVLECTGFFT
                     KREKAMAHIEAGAKRVIISAPAEDSDLTIVLGVNENMYDPAKHVVVSNASCTTNSLAP
                     VTKILNDAFGIEYLMGTTIHAYTSTQVLVDVPKGGGRKGRAAAVSLVPATTGAAKAMV
                     PLFPELKGKMDMISVRVPVADGSLTDIVVHFKKEVTVESVNAALKSAADGKLKGIVEY
                     NDEEIVSADIIGNTHSGIVDAPSTKVIMGKVAKVMVWYDNEYGYSNRMVELAQYIAGK
                     E"
     gene            complement(7275..8828)
                     /locus_tag="PK849_02290"
     CDS             complement(7275..8828)
                     /locus_tag="PK849_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPE64982.1"
                     /translation="MAVYLKRKINRRKKTEEELLRQTEYWKKLFEHSPQGILIYDGAG
                     QVKEANSRFREMFRLSAPAPLPENITSLLSGTEADFSRNDIFPQEENISGTAAPAKET
                     ALPDGDGGTLSVSFQAFPLSSNDEETHYCALFHDIRDRKTMEQKLHRRTILQERMTAI
                     SSRFVLFQNFRDSMEAALEDILALSGARTASLFVISDSGILIPEMEAFSPDKAGPSLA
                     ANFSDGDDFFWKSLLLNDALPSVITIESAVHKVRESKWDFLKEMGIDSITILPLFVKT
                     SFQGFLALTDPLQQWVDMSNDPLLGLFSDLMAMAIERRKEEDSLRETHERIHDRFTGA
                     ITALCQVSELRDVSTSGHQKNVSVLAEDLANEMQLPEEVRMGVRYAGLVHDLGKLYIP
                     AEILGKPSRLSEAEYELVKKHPEFGHDILSLLNFPWPLAEIVLQHHERVDGTGYPYSL
                     KRKDIRIEARILSVADAFEAMTSDRPYRKRKTAKAALKELMELAGKAYDAKIVKSFVK
                     MILERGENP"
BASE COUNT         2000 a         2169 c         2540 g         2187 t
ORIGIN      
        1 catcctcggt gattccactt ttttccattc cggtattacg ggccttatcg acgcggtggt
       61 gaacaagtcg aggggcgtgt tcgtcatcct tgacaaccgg attaccgcga tgtccggaca
      121 gcaggagaat cccggcacgg gaaggacgct catgggcgag gagacgaccg ctttggacat
      181 cccgcgtctc tgcgcagcgt gcggagtcaa ggaggaccat atccacgtcg tcgatccata
      241 taacctgaag gcgtgccgcg atgcagtgga tgtagcgtac gccgcagaag aaacatcggt
      301 gatcgtgacg acccgtccct gcgctttgat caaagaggtt cagaaaagca gggcctcgct
      361 ccactgcgtc gtcgatgcgg agaagtgcgt tctctgctct tcgtgcctca aactcggctg
      421 tccggcgatc acgaaacagg aaggccggat tgtcatcgac tccgtcgcct gcaacgggtg
      481 ttctttgtgc gtgcaggtct gcccgaaaca ggctatttcg agggaaggtg agcttcatga
      541 ataagaacgg aaagaacgtc cttctggtcg gcgtaggcgg tcagggaacg attctcgcga
      601 gcaagctcct ctcggaagga cttcttgcac tcgggtatga cgtgaaaatg tccgagatcc
      661 acgggatggc gcaacgaggc ggcagcgtct ccacgcatgt ccgctacggc gccagggtct
      721 actctcccgt gatgggtgcg ggcgaggcgg acgtcctcgt cgctttcgag aagatggagg
      781 cgcttcgttg gctttcctac ttgtctcctg aaggcatgct catcgtgaat aatcatgaga
      841 tctacccgat gcctgttatc ctcggcatgt cggagtatcc cgctcgactt gaggagacgc
      901 ttgccgaagc cgcgactcga atgcttttga ttgaagcggc ggatgaggcg gcggcattgg
      961 gcaatccgcg ggtgagtaac gtcgttttgc tcggagctgt ggtgaagagt ctcgggcttg
     1021 aagatccatg ctgggcggaa actcttcgtt cgtgcgtccc gcctcacgcg ctccaggtca
     1081 acgagagggc tttccgaaaa gggcttagcc tggtatagct acaaaacgaa tacagcagag
     1141 caggagaatg gggagctttc tccctgttct ccttttttta caagccaaag gtgcgttcgt
     1201 attccatata tacgaccgtg agcatcgtat gcgggcaata gcatgaaaac gagtccatcg
     1261 caggatatct ctttcagtcc tgcggtatcg gttcaggttt tgagtcgcaa ctcgtggaaa
     1321 taccctttga gaaaaacgtc cgcgaagggt aatatagact gcatactgca aagaatgcgg
     1381 ggtggagcgt gtgattgatt ttacaaaggg gtattctttc aatgagtacc ttgaaaaagg
     1441 gtacgaagaa gagcgggaca gacagcggcg cgcatgttcg cgtgccgtct tttctccggc
     1501 attcgaagag gcggctcgga atgtgaacaa acctctcaaa ttggctgcgt ttgccgaaat
     1561 ttactgcccg gactcggttg tgacaatgtc ttttgtccac cggatagcgg agctgaaccc
     1621 aaagattgaa cttgcggttt ttccgcgatc tccgtttgaa aaggagctgg aagcgctgac
     1681 aggttcctcc aggattccta cgcttctctt tttcgacgaa aaaatgaacc ttgcaggcag
     1741 ctacgtagag cttccaaaag cgttgaaaga ggatatgcgc gatctcgacg ccggagagaa
     1801 aagcgctctt actctcgatt acaggagagg aaagtataac gacatactcc aggccgagct
     1861 tacggagata ctctcactgt ttgcttcatg acgttctccg cgtacgaagg atttggagcg
     1921 cgttcggggc gtcttttttg agtggccggg tgttttggga ggtcgatgaa tgccgctctc
     1981 ttcaattttt ttattgtttc ttcgaatcgg cgcctttacc tttggcggcg ggttggtcat
     2041 gctggggttt atcgaaaaag agctgcgatc aactgcgcgg ttgtcgtccg aggaaatagc
     2101 ggacatgatg gtacttgcta cggcgtttcc cggtccggtt gccgccaata tggcattcct
     2161 ggcagggaaa cgccttgctg gcattgccgg ttcgttttgc gctctattag gcacggtcat
     2221 ccctccgttt ctgacaatcc tgctgttgtc gaaagcggtg atccatttca tgaacgaacc
     2281 ctggcttgca gcttttttcc ttggcgcggc ctgcgcagtc gtcgtcatta tcggaagggt
     2341 cgtctacagc atgactcgca tctcctgcgg aggaagatgg aaagatatag cggtgttcgc
     2401 ggcggtgggg attctgctta tcagcactga tgtacacccg ttttttgccc tcgcgggggg
     2461 agtgactgtt cgtctgctcc tcggggaaga ggatgccgga tgagtgttct tggaactctc
     2521 gcacttgttt ttgcccgcat aggcctgggg gcgttcggcg gaggcttggc gaccataccc
     2581 ttcattcatt ttgaacttgt cgcctccaga gcgtggcttt cagagagagg cttcgctgaa
     2641 gttgtttcat tggcgcagat gacgcccggc cctctcgccg taaatgctgc aacctttgtt
     2701 ggctacagga tcgcaggcgt tgcgggcgca tctgttgcaa cattctctgt cattgcagct
     2761 ccgcttcttg tgctcggctt ggcgcttttc ctgatttcca gagcatcgga gaaatggaaa
     2821 acgagagtcg atcgattaca gcgtgcactt cgccctgctg tatcgggaat gctcttcgcc
     2881 gccttctgga tggtagcccg ctctctgaca ggagactgga ggctctgggt gatgacagca
     2941 ggagtattca tgctcatcgg ccaagagtac ttcaagcgat atcctcaatt actgcttctt
     3001 attgcgggga tggctggagt gctcttcctc tgaacgaaaa aagaaaggga tgatggagaa
     3061 tggagatgaa aatcgctcgc gttaatctca cggaatcgac aatagaaatt ttctctgccg
     3121 aggacgaact tttacgaaag taccttggag gatccggact tgggacgtac taccttttca
     3181 agtacggctc gccgaagtgc gcggcgctct cccctgaaaa tcctctgctt ttcatgaacg
     3241 gtcctttcca agggacagga attcctactt cgggaagaca tcaggtgctc gcgaaatccc
     3301 cgcttaccga tatgttcgga gaatccgact gcggaggcgc ttttgggtat catatgcgca
     3361 aggccggttt tgatggcatc attcttcttg gcaaggcgcc ctctccggtg tacatagccc
     3421 tccttgacgg aaaaatcgag attcgcagcg cgtcctctct ctggggaaag gacacgttcg
     3481 agacggagga gtttttgttg gagctggagg gaaaaccggc cggtgttgcc tgtataggtc
     3541 ctgcaggaga gaatcaggtc ttgcttgcaa atatcatgca cgacggtcga aatgccagag
     3601 ctgcgggaag aggcggtatg ggggcggtaa tgggctccaa aaatttgaaa gccatagttg
     3661 cccgcggcag cgcaaaagtg ctcatggcgg acgaaaaggc tgtccgagag aagtctgctg
     3721 aaaaggcgaa gtactatatg gaaaagcttc ccgcgatgac ccgtttcggt accgccgggg
     3781 gagttgcgtt ggctgagcag aacggagatc ttcccctgaa gaactggagt atgggctcct
     3841 ggacggaaga tgtgaagtcc attaccggtg aagcaatggc cgagacgata ctgaccggca
     3901 actggggctg catgtcctgc ccgatacgat gcggccggga tgtagagttc gacggaataa
     3961 agggcgccgg gccggagtac gaaacgatcg gtatgctggg aagcaactgc ctcatcagcg
     4021 atctgaaggc cattgcgcgt ggaaacgacc tgtgtaaccg aatgggtatc gacacgattt
     4081 caggaggttc ggctgtcgct ttcgccatgg agttgtacga aagagggata atttcggaag
     4141 aggaaattgg ctacccgctt ttgtggggtg acggggagtc aatgatacgt cttctatcgg
     4201 atattgtaga aaagcgagga ttcggcgcgc tgctgtccga gggaaccaaa aaggctgcgg
     4261 agcgtatcgg aaaaggggcg gaacggtatg ccattcattc caagggaatg gatttccccg
     4321 cgcatgaccc ccgctgttat aagagcttgg caacgggata cgcaacatcg aaccggggag
     4381 cctgccatct ttccggtttt tcctattcgt ttgaacggag catgacctat ccggagttcg
     4441 gcgttgatac ggtgatggat aggacggtcg atgaagggaa aggggctgtg aatattccct
     4501 tccagaacat gatgggcgtt cttgattcct tgaaaatgtg caagttcccg ttcgcaatca
     4561 caaggataga tgatattctt caatggatca acggaatcac ggggtggaat atgagcggcg
     4621 atgaactcct tcttgcggga gagaggattt tcaacctcaa gcggcttttc aatgtagcct
     4681 gcggagcctc ggcgctggac gatactcttc cggagagaat tcttacggaa cctcgcggaa
     4741 gcggcggttc ggcgaacact cttccggatc tgaagttgca gctcaaagag tattatcctg
     4801 aaagaggctg gtctgaagat ggtattccta ccgaggaaaa actgaaatct cttgatttgg
     4861 aagagttctt atgctgtgta aacgggcctg ctctcgaatg atacgctttg gggaaaagga
     4921 gtgaattatg gtacataaga ttgtccgtta ctccgttcta ttgtttattc tcgtaccgct
     4981 gctcacctgg tttgcgtcgc gaggagccga catggttgcg ctcgccaggt ggaatacgga
     5041 agattccgcc atcgtcatcc ttgaaaaggt actcggcagg gaggacgacg ttgaaagtgg
     5101 agccgaaggc aactgggaga cagagcgcgt acacatacga attacctacc gtaacggtgc
     5161 gttcgccggc acgactgagg agatcgaaat ccttcagctt gcggatagcc ggctggagct
     5221 tgttcctggg agagaatacc tctttcttgt cgatacgttc gacgacggaa cacatcagta
     5281 ttctataagc gacagataca ggatacccgc tgtcgccggc tttatcacct tggcctgcgc
     5341 agcgcttgcc gttcttgcgg gtacgtcggg ggtaaaggct ctctgcggtc tgtttctgtc
     5401 gcttttcctg ttacttggat ggtttgttcc gatgcttgcc gaagggtata cgcctgtgcc
     5461 tttcgctatc ttggccatag ccggggtttc tctgctgacg atagtcttcg tcgttcccaa
     5521 acgcgctctt tggccaattc ctttcatcgg ggccgtagga ggagcaatcg gagcctcggt
     5581 tacgggctgg attatggttg ggctgtggca gctgaccgga ctggagaacg atagcgccgt
     5641 gcttcttttt tccgttgcgc ctcaccttga actgcggggg cttcttcttg ccgcagtgat
     5701 ggtcggcggc ataggagctg tgcttgacgt agcggtttcg gtgacctcgt cgatggcgga
     5761 gctgtactcc tacgatccct cgatcccgct acgaagatta tggagagccg ggatcaacgt
     5821 cgggagcgat gttctcggca gtatgatcaa tacgttgata cttgcctacc tcgggagttc
     5881 gctcccgttc gtcgttctta tctcgatgga gggcccagat ttggtcgcac ttctcaacga
     5941 tccgcacatc gcacaggaga ttttgcgcag tgttgccggc acggtaggac tgcttctcac
     6001 aattcctgtg actgccggcg ccggcgtatg gtggatagca cgttctcata tcccggatga
     6061 gaaaagacac aacgaagagc ttgctgactg accgttcact ttgtgataag atatggcaat
     6121 gtagattaaa gctattcata cacttctgga ggtgttgcaa tgaaaaaaat agcaatcaac
     6181 ggacttggaa ggatcgggcg gcttgttctt cgctgctata tggcgaacaa gcctgccgat
     6241 gttgagatcg tcgcattgaa cgatctgacg ccggcctcgg agatggccta tctgataaag
     6301 ttcgactcta tccacagaaa agccaacttc tcggtcgaag ctcgagagaa cagcctggta
     6361 cttgacggga aagaaatccc tctttttaag gaaaaggatc ctgcaaaact cccctggaaa
     6421 gagcttggtg tggatattgt tcttgagtgc actgggtttt ttacgaagag agaaaaagcc
     6481 atggcacaca tcgaggccgg agcgaagcgg gtcattatca gcgctcctgc tgaagactcc
     6541 gaccttacca tcgtcctcgg cgtgaacgag aatatgtacg acccggcaaa acatgtcgtc
     6601 gtctccaacg cctcttgcac gacaaactcc ctggcgccgg tgacaaagat tcttaacgac
     6661 gctttcggca tcgaatatct gatgggaaca accattcacg cctacacatc gacacaggtc
     6721 ctcgtcgatg tacccaaggg gggcggcaga aaaggacgtg ccgctgcagt ttctcttgtg
     6781 cctgcaacaa cgggtgcggc aaaggctatg gttcctcttt tccccgagct taaaggcaag
     6841 atggacatga tctctgtacg tgttccggta gccgacggat ctctcactga tatcgtcgtt
     6901 catttcaaaa aggaagttac cgtagagtca gtcaacgcgg cgctgaaatc ggcagccgac
     6961 ggaaaactga agggaatcgt cgagtacaac gacgaagaga tcgtctccgc cgatatcatc
     7021 ggcaacacgc actcgggtat tgtcgacgcg ccttccacca aggtgatcat gggcaaggtc
     7081 gccaaggtca tggtgtggta cgacaacgag tacggctatt cgaacaggat ggtggagttg
     7141 gcgcagtaca tcgcgggcaa ggaataaccc atctttcaca gttcgttttg caagcgcccg
     7201 agtcccccga tgggagctcg ggcgcttgcg tttttacaaa gattcagtgc gcgctgtccc
     7261 agttgcttgt actcttaagg attttcccca cgctcaagaa tcatcttcac aaacgacttc
     7321 actatctttg cgtcataggc tttccccgca agctccataa gttccttcag agcggctttc
     7381 gccgtctttc tctttctgta ggggcgatcg gatgtcatgg cctcaaaagc atctgcgaca
     7441 gacaagatac gcgcctcaat gcgaatatct tttctcttca gggaatacgg gtagcccgtt
     7501 ccatcaacgc gttcatggtg ttgaagaaca atctccgcca atggccaggg gaaattcagc
     7561 agggagagaa tatcgtgtcc gaattcgggg tgttttttta cgagctcgta ctcggcttcc
     7621 gagagccggg agggctttcc caggatttcg gcaggtatat agagtttacc taaatcatgc
     7681 acgagtcctg cgtacctgac ccccatccga acctcttcgg ggagctgcat ttcgtttgca
     7741 aggtcctctg caagaacaga aacatttttc tgatgtcccg atgtggagac gtccctgagt
     7801 tcgctgacct ggcagagcgc tgttatggct ccggtgaatc tgtcgtgaat tcgctcatgc
     7861 gtctcccgaa gggagtcctc ctctttgcgt cgctcgatag ccattgccat caggtcggag
     7921 aaaagaccga gcagagggtc attggacatg tctacccact gctggagagg gtctgtgagc
     7981 gctagaaatc cttggaaact tgtcttgacg aaaagaggga gaatcgtgat ggagtcgatc
     8041 cccatttcct ttagaaaatc ccatttactc tctcttactt tatgtactgc tgattcgatg
     8101 gtgatgacgg agggaagggc gtcgttcaat aggagagatt tccagaagaa atcgtcgccg
     8161 tcggagaagt ttgcggcaag agaggggccc gctttgtccg gagaaaacgc ttccatttca
     8221 ggaatcagaa ttcccgaatc ggaaatcacg aaaagcgagg cggtacgcgc tcccgaaagt
     8281 gcaagtatat cttcgagcgc ggcttccatg gagtccctga agttctggaa gaggacgaat
     8341 cgagaggaaa tagctgtcat tcgctcttga agaatcgttc tccgatggag cttctgttcc
     8401 atcgttttcc tgtctcttat atcatggaag agagcgcaat aatgagtttc ctcatcattt
     8461 gacgacaggg gaaaggcttg gaatgagaca gagagcgttc cgccgtcccc gtcaggaaga
     8521 gccgtctcct ttgccggcgc ggctgtacct gaaatgttct cctcctgtgg aaaaatgtcg
     8581 tttcgcgaaa aatcagcttc ggttccggag agaagcgacg tgatattctc tggaagcgga
     8641 gccggcgccg aaagacggaa catttcccgg aaacggctgt ttgcctcttt tacctggcct
     8701 gcgccatcgt agataagaat accttgagga gagtgttcaa agagcttttt ccagtactct
     8761 gtctgtcgaa ggagctcctc ttccgttttt tttcgtctgt tgatctttct cttcagataa
     8821 acggccaatc ccgcaaggaa aagaaggaga aaaaggtttg cgaaaataaa aggagtatac
     8881 tcctggatat tataaa
//
LOCUS       DAOPKU010000042         8852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8359_length_8852_cov_4.597363, whole genome shotgun sequence.
ACCESSION   DAOPKU010000042 DAOPKU010000000
VERSION     DAOPKU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8359_length_8852_cov_4.597363"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PK849_02295"
     CDS             <1..567
                     /locus_tag="PK849_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPE64983.1"
                     /translation="MDSEGRTSIPGLFVCGEVSGGVDGASRMGGNALTNILVFGLRAG
                     KTAAEEAMASSAVSDAVSKLPECVLRSGREGVSPAEVRCELRSIVQEGLSPWRNAEGI
                     ERCISKIELLNASRRALNIASSLDLLHAMELHGLHRTAEAVARAAFTRKESRGVHFRE
                     DFPNESEEWKRRIRILLEEGAVTARVGE"
     gene            complement(641..1819)
                     /locus_tag="PK849_02300"
     CDS             complement(641..1819)
                     /locus_tag="PK849_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE64984.1"
                     /translation="MRRMHSGIFCVIFALVLLFAAASSAATPSGDPIKIGYLATLTGD
                     GATWGQHERDGALLAVKELNEAGGVLGRPLELVYYDVRGRQEDAIQAARRLIHEDKVV
                     AIGGTNYSGLNIAIASVVNSGEVPQIGTASTNPAVTVDPKTNTVRPYTFRLCYTDPYQ
                     GKVMADYLVNKLGVKSTAVITDIGSDYSEGLKEFFLARIEELGCEMKGVWGFRGGDVD
                     FRAQLTEMKATGAEAVALPILYKEMGLIMKQAAELDWKPIFIGGDGFSPSMHEIAGDA
                     MEGSYWVYSMDLGNPQMLYLLSRYEKEYGDPAAEPGNVAYAYDLVTWVADAIRRAGTA
                     DPKAIRDAMENTQNLRLVHFTLTVDKATHNPLNKPAAMMLWKNQLFNFLEMWAPQDKF
                     "
     gene            complement(1981..5307)
                     /locus_tag="PK849_02305"
     CDS             complement(1981..5307)
                     /locus_tag="PK849_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE64985.1"
                     /translation="MKKNIKVTLNGKTVYGYEGQRILDLCTECGVEIPTLCYDPHLSL
                     HGGCSVCLVEVEGAKTLLRACANTISQGMIIKTETERAVSARRTALELLLSDHVGDCR
                     PPCTLTCPANGNVQAYINLAAQGKYDESLDVLHHHVTLPACIGRVCPAPCEKKCRRNF
                     VDDAPVSIREIKRFVGDWAIRNEKMGFIPEIEENGKRIAIVGGGPAGISAAYYLRLKG
                     YAPVIFEKEMLLGGMMRYGIPDYRLPQQVLQAEIDWLLAHGVEVRTGMTLGDNITLEA
                     LRKEFDGVILAMGCWKSSPMRVPGEDLEGVLGGINFLYDVKTNPSVTIGRRVAVVGGG
                     NTAMDACRSARRLGAEQVSVLYRRSREEMPADNIEIEEATEEGVDFIYLAAPKAIEGN
                     GKVERILCERMQLGEPDASGRRSPVPTGETFILEVDTVIAAVGQGIDFSTVPAELHDG
                     RKMKVGKDYDTPLPGVFVCGDQQTGPKIAIEAIGNGHWAADSLDHYLTHGTPKKPFFY
                     DIVQTDLGPEDFADVERTTQEHVAHVSGETRLAAPFKEYSKGLTEEQTLRDAKRCMEC
                     GCPDVFECKLRKFATTHEVQPEKLAGAHIEKFEDANQYYVRNMDKCILCAKCVRACDE
                     ISGFHAIDFAKRGFESVLTPHFFNDMEHSDCTFCGLCTQVCPVGALMEKRVERWPHLE
                     EPTIVKTTCMLCPVGCELDLNLDRKRSRIVRVTTDLDNPEAPTGGSCCVKGRYAFKDV
                     ADDGAFYPALKGACSSWSDAVSAFEELASGNSPAFVLGPSLTLEEVRALKEYTALRAP
                     NAEMYAIWDAGFISLLNEAAKRKDVVRATYASMTGPSPSSDIRCGEADAFLLVGTNTD
                     KDQPVLTSWLRRSARHRKSAVVYIGETPGLLDKGEAVVLHPEAGKEAQVLTALTSAVK
                     AITAKESVASTNLLSGTGVLPEDLEKAAGLLAGAKHPVTLIGAEASAKEVFDAAAALA
                     GGRYMLLFKGSGSVALISAIPGILPAATLRTVQKNMGALLFVGTTPEDAGFTADSLTE
                     RKYAVLSSVSSPLAEKADVLLPLLPWIEKDGSITNLEGRTLSVRRGPLTKKTGKSLSA
                     LLAEAALPYEGRIHANPVAR"
     gene            complement(5335..7101)
                     /locus_tag="PK849_02310"
     CDS             complement(5335..7101)
                     /locus_tag="PK849_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPE64986.1"
                     /translation="MTKTIVKVGLASCGVAAGAVPIYETLLSALKGHDDVEIKKVGCI
                     GLCYMEPIVEIERDGKSIAYGKVDTELAKEIAESHALRGEIVQRAVILDPSSKACENE
                     RMDSQVRIVLRNSGLIDPEKIDEYVARDGYGALKKALSMTPEQVIEEVLASGLRGRGG
                     AGFPTGLKWKFARGAKGDKKYFVCNADEGDPGAFMDRSVLESDPHSIIEGMTIGGYSI
                     GSDEGIIYCRAEYPLAIKHLNVALEQAREKGYLGKNILGSNFSFDIHIKEGAGAFVCG
                     EETAMIASIEGRRGMPRPRPPFPANSGVFGKPTNINNVETIANIPWIILNGAAKFSEY
                     GIGKSRGTKVFALAGKIKKGGLAEVPMGMPLREVIFNVAGGIAENKKFKAVQLGGPSG
                     GCIPESLLDTPVDYESINATGAIMGSGGMVVMDETTCIVDVAKFFLAFVQKESCGKCP
                     FCRIGTKRMLEILERISEGKGVMEDLDTLLDLAYQVKEGSLCGLGQTAPNPVLTTLKY
                     FREEYEAHIRDKKCPAKVCTSLIHYVIDPAVCIGCTKCARNCPVSCIAGEVKKPHVID
                     DEKCIRCGQCKKVCPVGAISVE"
     gene            complement(7120..7629)
                     /gene="nuoE"
                     /locus_tag="PK849_02315"
     CDS             complement(7120..7629)
                     /gene="nuoE"
                     /locus_tag="PK849_02315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPE64987.1"
                     /translation="MSTEASVSSALEQRLLPIADRYRGRKGITIPLLADLQKEMGYIA
                     PEAVDFIAHELDIPAAEMFGVATFYAMFRLQPEGKHVIRLCRGTACHVQGSARIGEQI
                     QRHLSVSDGGTTSDGVFTLQFVACLGCCSLAPVMMVDGEVFGLLTPEKAVDVLESVRS
                     ASSGKGAAD"
     gene            7811..8047
                     /locus_tag="PK849_02320"
     CDS             7811..8047
                     /locus_tag="PK849_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPE64988.1"
                     /translation="MITVYSTKTCPWCTKVKEYLEGKKIPFEVIDVASDREAAMEMVK
                     KTGQMGVPVTRIGEKYIVGYNPEAIDEEIAQQNQ"
     gene            8073..>8852
                     /locus_tag="PK849_02325"
     CDS             8073..>8852
                     /locus_tag="PK849_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPE64989.1"
                     /translation="MTRGTRSGSDLLTVSFGMAVDSGADSASTRGVSVEALKECGDSL
                     AKADAWLREGRNEGRDGVGWLSLPYKDIGDIQEAAGWLRGFNAVVQAGIGGSALGNLM
                     LHGALLPPYWNELPEAKRGGPRFYLADNVDPRDNRTVWNLIEPEKTAFIVISKSGSTA
                     ETMANFLFFWDRLKVELGPKRAAEHVLVITDEEKGVLRPFADEIGCRRLVLPSDVGGR
                     FSVLSSVGLLSACALGIDGRKLLAGAAEMDEKLAQRASLFEN"
BASE COUNT         1747 a         2488 c         2641 g         1976 t
ORIGIN      
        1 atggattccg aggggagaac ctcgattcca ggcctcttcg tctgcgggga ggtttcggga
       61 ggtgttgacg gcgcgagccg catgggagga aacgccttga cgaatatcct tgtcttcggt
      121 ctccgtgcgg ggaaaacggc cgctgaagag gctatggcgt cttccgcggt ttctgacgcc
      181 gtaagcaaat taccggaatg tgtgcttcga tcggggcggg aaggcgtctc tcccgcggaa
      241 gtacgctgcg aactccgcag tatagtccag gaagggcttt ctccatggcg gaatgcggaa
      301 gggatcgagc ggtgcatctc gaagattgaa ctcctgaacg cttcacgaag agcgctgaat
      361 atcgcgtctt ctctcgatct tctgcacgcg atggaattac acggtctcca tcgtaccgcg
      421 gaggcggtcg caagggcggc cttcacccga aaggaaagcc gcggagtcca tttcagagag
      481 gacttcccga acgagagtga ggagtggaaa cggcgtatca ggatacttct ggaggaaggc
      541 gcggtaacag ccagagtcgg ggagtgaacg ccgagtatga acagaaaaac cccgcctctt
      601 ctgaagatga ggcggggttt ctgtaaaagg ttccaaagaa ttagaatttg tcctgcggag
      661 cccacatttc aaggaagttg aacagttgat ttttccagag catcatcgcg gcaggcttgt
      721 tcagcgggtt gtgcgtcgcc ttgtccacgg tgagcgtaaa atgaacgaga cgaaggttct
      781 gcgtattctc catcgcatcc cggattgctt tcgggtcagc cgtaccggcg cgtctgatcg
      841 cgtcggcaac ccatgtgacc agatcgtatg cgtacgcaac attcccgggt tcggctgcag
      901 ggtcgccgta ctccttctcg taacgggaga gaaggtagag catctgcgga ttgccgaggt
      961 ccatgctgta gacccagtag ctcccctcca tcgcgtcgcc ggcgatctcg tgcatactcg
     1021 ggctgaagcc gtcgcctccg ataaaaatcg gcttccagtc gagttccgcc gcctgcttca
     1081 taatcaggcc catctctttg tagagaatgg ggagcgcgac cgcctcggcg ccggtcgctt
     1141 tcatctccgt cagctgggcc cggaagtcga cgtcgccgcc gcggaaaccc caaacaccct
     1201 tcatttcgca gccgagttct tcaatgcgtg cgaggaaaaa ctccttaagc ccttcggagt
     1261 agtcgcttcc aatgtcggtg ataacggccg tgcttttgac gccgagcttg ttgacgaggt
     1321 agtcggccat gacctttccc tggtaggggt cggtgtagca gagacggaac gtgtaggggc
     1381 gcactgtgtt tgtcttcgga tcgacggtga ctgcggggtt ggtgctcgcg gtacctatct
     1441 gcgggacttc gccgctgttc acaacggagg ctatggcgat attgaggccg ctgtagttcg
     1501 tccccccgat cgcaacgact ttgtcttcgt gaatcaaacg gcgggccgcc tggatagcgt
     1561 cctcctgtcg tccgcggacg tcgtaataga ccagttcgag cggccgtcca agaacgccgc
     1621 ccgcctcgtt cagctctttg accgcgagca gcgcgccgtc gcgttcatgc tggccccaag
     1681 tggcgccgtc tccggtgagc gtcgccagat acccgatctt gatcggatct cccgacggtg
     1741 ttgccgcaga agaagcggca gcgaaaagca gtaccagcgc gaagatgacg caaaaaatgc
     1801 cactgtgcat tcgtctcatg aatttctcaa cctccctgat atgtgttaaa aaaatatttg
     1861 gttaattcta aaggccgttg ccctttgtgt caatgcggct aataaaaaga aacaaaaatt
     1921 cacagggccg gaaggccctg tgaattttga tgacgctcgt tctttttttg tgtttttgta
     1981 tcagcgagca acaggattgg cgtgaattct tccctcgtaa ggcagagcgg cttccgcaag
     2041 aagggcggag aggctctttc ctgttttttt agtcagaggc ccccgtcgga ccgagagcgt
     2101 tcgtccttcg aggttcgtga tgcttccatc cttttcaatc catgggagaa gcggtagcag
     2161 aacgtcggcc ttctcggcga gcggagagga tacggacgaa aggaccgcat actttctctc
     2221 tgtcaggctg tccgcggtga agccggcatc ctcgggagtc gtccctacga agagcagggc
     2281 gcccatgttc ttctgaactg tgcgaagagt tgccgcagga agtatcccgg gtatggccga
     2341 gatgagcgca acgcttcccg atcccttgaa gagaagcata tatcgtcctc ctgcgagcgc
     2401 cgcagccgcg tcgaaaactt cttttgcaga cgcctccgcg ccgatgagcg tcacgggatg
     2461 tttcgcaccg gcaaggagtc cggccgcttt ttcaaggtct tcgggaagaa cgccggttcc
     2521 tgagaggaga ttggtcgacg cgacgctttc cttggccgtt atcgccttga ctgcggatgt
     2581 gagcgctgtg aggacctgcg cttcttttcc ggcttccggg tgcaaaacta cggcttcgcc
     2641 tttgtccaga aggcctggag tctcgccgat gtagaccacc gctgatttgc ggtgacgggc
     2701 agacctgcgg agccaggatg tgagcaccgg ctggtctttg tcggtgttcg ttcctacgag
     2761 caagaaggcg tcggcctctc cgcagcgtat gtcgcttgac ggggaggggc cggtcatgga
     2821 ggcgtaggtc gccctgacca catccttccg cttcgccgct tcattgagaa gggaaatgaa
     2881 cccggcatcc caaatcgcgt acatttccgc gttcggagcg cgaagggccg tgtactcctt
     2941 aagagcgcga acttcttcga gtgtgagcga tggaccgagt acgaacgcag ggctgttccc
     3001 ggatgcgagt tcctcgaaag ccgagacggc gtcgctccag gaggagcaag ctcccttgag
     3061 cgcagggtag aaggcgccgt cgtcggcgac gtctttgaag gcgtagcgcc cctttacgca
     3121 gcaggaaccg cctgtcggcg cctccggatt gtcgagatct gtggtaacgc ggacaatacg
     3181 ggaacgcttc ctgtcgaggt tcaagtcgag ctcgcagccg accgggcaga gcatacatgt
     3241 tgtcttgacg attgtaggct cttccaagtg cggccagcgt tcgacgcgtt tttccataag
     3301 cgccccgacc gggcagacct gggtgcagag gccgcagaaa gtgcagtcgg agtgttccat
     3361 gtcgttgaag aagtgcggtg tgagtacgga ctcgaagccg cgcttcgcaa agtcgatagc
     3421 gtggaaaccg gagatttcgt cgcatgcgcg gacgcacttc gcacagagaa tgcacttgtc
     3481 catgttccgt acgtagtact ggttggcgtc ctcgaacttc tcgatgtgcg cgcccgccag
     3541 cttctcgggc tgtacttcat gcgtagtcgc gaacttgcgg agcttgcatt cgaagacgtc
     3601 ggggcagccg cactccatgc agcgcttcgc gtcgcggagg gtctgttctt cggtcagccc
     3661 tttactgtac tccttgaagg gggcggccag ccgtgtttct cctgatacgt gcgcgacgtg
     3721 ctcctgggtt gtgcgttcga cgtcggcgaa gtcctcgggg ccgaggtccg tctggacgat
     3781 atcgtagaag aacggtttct tgggcgttcc gtgcgtcagg tagtggtcca gcgaatccgc
     3841 cgcccaatgt ccgttgccga tcgcctcgat ggcgatcttg ggtcccgtct gctggtcgcc
     3901 gcagacgaag acgccgggaa gcggcgtatc atagtctttg ccgaccttca tttttctgcc
     3961 gtcgtgcagc tctgccggaa cagtggagaa gtcgattccc tggcctactg ccgcgatgac
     4021 ggtgtcgacc tcgagaataa aggtctcgcc cgtgggaacg gggctccgtc tgccggaggc
     4081 gtcgggttcg ccgagttgca tgcgttcgca gagaattcgc tcgacctttc cgtttccttc
     4141 gattgccttc ggcgcggcga gatagatgaa gtcgacccct tcttcagtag cctcttctat
     4201 ttctatgttg tcggccggca tctcctcgcg ggagcgccgg tagagcacgg agacctgctc
     4261 cgcgccaagg cggcgggccg aacggcatgc gtccatcgcg gtgtttccgc cgccgacgac
     4321 cgcgactctt ctgccgattg tgacggaggg atttgtcttc acatcgtaca ggaagttgat
     4381 gcctccgagt acgccttcaa gatcctctcc cggaacgcgc atgggggagg acttccagca
     4441 ccccatggcg aggatcacgc cgtcgaactc cttccggagc gcttcgagtg tgatattgtc
     4501 gccgagggtc atccctgtgc gcacctccac gccgtgcgcg agcagccagt cgatctccgc
     4561 ctggaggacc tgctgcggca ggcggtagtc ggggatcccg taccgcatca taccgccgag
     4621 gagcatctcc ttttcgaaga tgaccggggc gtaccccttc agccgaagat agtaagcggc
     4681 ggaaataccg gcggggcctc cgcccacgat agcgattctc tttccgtttt cttcgatctc
     4741 gggaatgaat cccatcttct cgtttctgat cgcccagtcg ccgacgaatc gtttgatttc
     4801 ccgtattgag acaggcgcgt cgtcgacaaa gtttctacgg catttcttct cgcagggggc
     4861 ggggcagacc ctgccgatgc acgcagggag cgtgacatgg tgatggagca cgtcgagcga
     4921 ctcgtcgtac tttccctgcg cggcgagatt gatataggcc tgcacgttgc cgttcgcagg
     4981 gcaggtgagc gtacatggag gacggcagtc ccccacgtgg tcggagagca gcagctcgag
     5041 tgcggtacgg cgcgcgctga ctgcacgctc agtctcggtt tttatgatca ttccctgcga
     5101 gatggtgttg gcgcacgcgc gcaggagcgt cttcgcacct tcgacttcga cgagacagac
     5161 agaacagcct ccgtgcaggc tcagatgcgg atcgtagcag agcgtgggaa tctccacgcc
     5221 gcactcggta cagaggtcga gaatccgttg cccctcgtag ccgtagacag tttttccgtt
     5281 gagtgtgact tttatgtttt tcttcatggc agctcgaaat cctcccgaca atgcttattc
     5341 cacggaaatg gcgcccacag ggcagacctt tttacactga ccgcaccgga tgcacttttc
     5401 gtcgtcgata acgtggggct tttttacttc tccggcgata caggagacag gacagttccg
     5461 ggcgcacttt gtgcagccga tgcacacggc ggggtcgatc acgtagtgaa tgagactcgt
     5521 gcagaccttc gcggggcact tcttatccct gatgtgtgcc tcgtactctt cgcggaagta
     5581 tttcagcgtg gtaagcacgg ggttcggagc tgtctggcca agaccgcaga gagagccctc
     5641 ctttacctga taggcaagat caaggagcgt atcaaggtct tccatgacgc ccttcccctc
     5701 ggagatgcgt tccaggatct caagcatccg cttcgtcccg atccggcaga aggggcactt
     5761 cccgcaggac tccttctgaa cgaaggcgag gaagaacttg gcaacgtcga cgatgcaggt
     5821 cgtttcgtcc atgaccacca ttccgccgga gcccataatg gcgcccgtgg cgttgatgga
     5881 ttcatagtca acaggggtgt cgagcagcga ttcgggaata cagcctcccg aaggacctcc
     5941 aagctggacg gccttgaact tcttgttttc tgcgataccg cccgccacgt tgaagatgac
     6001 ctcgcgcagc ggcattccca tcggaacctc cgcgaggccg cctttcttga tctttcctgc
     6061 gagggcgaag acttttgtcc cccggctctt gccgattccg tattcggaga acttcgccgc
     6121 tccgttcaga atgatccagg gaatattcgc gatggtctcc acgttgttga tattcgtcgg
     6181 tttgccgaag acgccgctgt tcgcagggaa cggaggccgc ggccgcggca tgccgcgccg
     6241 gccttcgatg gaagcgatca tggcagtctc ctcgccgcat acaaaggcgc ccgcgccttc
     6301 ttttatgtga atgtcgaagg agaagttcga ccccaaaatg ttcttcccga ggtacccttt
     6361 ttcacgcgcc tgttcgagcg cgacgttcag gtgcttgatg gcgagcgggt actccgctct
     6421 gcagtagatg atcccctcgt ccgaaccgat ggagtagccg ccgatggtca ttccctcgat
     6481 gatgctgtgc ggatcgctct ccagaacgga gcggtccatg aacgcgcctg ggtcgccctc
     6541 gtcggcgttg cagacgaagt attttttgtc ccccttggcg ccgcgagcga acttccattt
     6601 gagtcctgtg gggaatcccg cgccgccgcg cccgcggagg ccgctggcga gaacctcttc
     6661 gattacctgc tccggcgtca tggacagcgc ctttttcaat gcgccgtagc cgtcgcgggc
     6721 gacatactcg tcgatctttt ccgggtcgat gagtccgctg ttcctgagaa cgatacgtac
     6781 ctgactgtcc atacgctcgt tttcacaggc tttggatgaa ggatcgagaa tcaccgcacg
     6841 ctgaacaatc tctccgcgaa gcgcgtgcga ctcggcgatc tccttcgcga gctcggtatc
     6901 caccttaccg taggcgatgg atttcccatc acgttcaatt tcaacgatag gctccatgta
     6961 gcagagtcct atgcagccga ctttttttat ctcgacgtcg tcgtgtcctt tcagggccga
     7021 gagaagagtt tcatatatcg ggacggcgcc ggcagccacg ccgcagctgg ccagaccgac
     7081 cttcacgatg gttttggtca ccttgcacac ctccatgagc tagtccgcag cgccttttcc
     7141 cgaggaggcg ctgcgcacgg attcaagaac atcgacggct ttttcgggcg tgagcagccc
     7201 gaaaacttca ccgtccacca tcatgaccgg cgccaggctg cagcatccca gacaggcgac
     7261 gaactgaagt gtgaacactc cgtcggaggt ggtcccgccg tcggaaaccg aaagatggcg
     7321 ctgaatctgc tcccctatac gcgccgatcc ctgaacgtga catgccgtcc cccggcaaag
     7381 gcggatcaca tgctttccct ccggctgcag gcggaacatg gcgtaaaatg tggcgacgcc
     7441 gaacatctcc gcagcaggaa tatccagctc gtgtgcaata aaatccaccg cttccggagc
     7501 gatgtacccc atctccttct ggagatccgc aagaagcggt atggtgatcc ccttccttcc
     7561 ccggtatcgg tccgctattg gaagaaggcg ctgctccaaa gcggaactca cggaggcctc
     7621 cgtcgacatg gtcattttct ctcctcctcg tgctttttca gtgaaatacc ccggtgatgg
     7681 tatttattat accgcgtatt gaggacaatt tcccatccaa aaagaaattc ggtatataat
     7741 aaatctcgac tgggagtgag atttgatata tactcccctc aagtatatat catttatgga
     7801 ggtgtctgtc atgataacag tctattcgac gaagacatgc ccgtggtgca ccaaggtgaa
     7861 agagtacctg gaaggaaaaa aaataccgtt tgaggtgatc gacgtcgctt cagaccggga
     7921 agcggccatg gagatggtca aaaaaacagg gcagatgggc gttccggtga cgaggatagg
     7981 ggaaaagtat attgtaggtt acaatccgga ggccatcgac gaggagatag cgcaacagaa
     8041 tcagtaagcc gttacaggaa ggagtggaga aagtgacgag aggaacacgt tcaggctccg
     8101 atctgcttac tgtatcattt ggaatggcgg ttgacagcgg ggcggattcg gcgtctaccc
     8161 gcggtgtgag cgtcgaagct ctgaaagagt gcggcgatag tctggcgaaa gccgacgcct
     8221 ggcttcggga aggacgtaac gagggaaggg acggagtagg gtggctttcc cttccctaca
     8281 aggatatcgg cgatatacaa gaagccgcag gatggttgcg tggcttcaat gcagtggtcc
     8341 aggcgggaat cggcggttcc gctctcggta atctgatgct gcacggagcg cttcttcccc
     8401 cgtattggaa cgaactgccc gaggcaaaac ggggcggtcc cagattctat cttgcggaca
     8461 acgtcgatcc acgggacaac aggacggtct ggaatcttat cgagcctgag aagacagcct
     8521 tcatcgtcat cagcaaatcc gggagcaccg ccgagacgat ggcgaatttt cttttctttt
     8581 gggatcggct gaaggtggag ctggggccga aaagagctgc tgagcatgtt cttgtcatca
     8641 ccgatgaaga aaagggcgtt ctgaggcctt ttgccgacga aatcggctgt cgtcgtcttg
     8701 tacttccttc cgatgtcggc gggcgctttt cggtgctctc ttcagtaggg cttctttccg
     8761 cttgcgcact cgggatcgac ggcagaaaac tcctcgcagg agcggcagag atggacgaga
     8821 agctcgcgca gcgcgcttct cttttcgaga at
//
LOCUS       DAOPKU010000043         8799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8438_length_8799_cov_5.256176, whole genome shotgun sequence.
ACCESSION   DAOPKU010000043 DAOPKU010000000
VERSION     DAOPKU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8438_length_8799_cov_5.256176"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /gene="tsf"
                     /locus_tag="PK849_02330"
     CDS             <1..479
                     /gene="tsf"
                     /locus_tag="PK849_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869491.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HPE64990.1"
                     /translation="LREKGLAKAAKKAARTAADGRVFSYIHTNGKLGALIELNCETDF
                     VAKTDEFQNLGHELCMHIAAAAPQFLSSEEVTADVLEREREIYKQQALEEGKPANIVD
                     KIADGKINKFYELNCLLEQNWIRDGDKKIKDLIVEVIAKLGENIVLRRFSRFSIGE"
     gene            555..1265
                     /gene="pyrH"
                     /locus_tag="PK849_02335"
     CDS             555..1265
                     /gene="pyrH"
                     /locus_tag="PK849_02335"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPE64991.1"
                     /translation="MKFNRVLLKLSGEVLAGNNGFGFDFDAVRRIGEEIVEVARSGIQ
                     IAMVVGGGNMLRGKELESLGVERSQADYMGMLATVMNALCLQDVLEKFGVPTRVQTAI
                     EMRQMAEPYIRRRALRHLEKERVVIFAAGTGSPYFSTDTAAALRAAEVGADCLLKATK
                     VDGIYSADPKKFPDAVLLHRLTYMEALQRQFEVMDAAAFSLCMENAIPIVVLNILRKG
                     NLKDFLVDGKNTGTMVSA"
     gene            1430..1987
                     /gene="frr"
                     /locus_tag="PK849_02340"
     CDS             1430..1987
                     /gene="frr"
                     /locus_tag="PK849_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPE64992.1"
                     /translation="MPKTELKELRERMEKVIEHLKNEFLAIRTGRAHPGLVSDIKVDY
                     YGAPTPVKQIATVSIPEGRQIAIAPFDRSALKLIEKAILASSLGVTPQNDGEIIRVNL
                     PELTRDRRIELTKLVGKYAEEGRIALRNLRRDSNDVLKKKEKNSEISEDDLKKYTKEV
                     QDIIDEYIKKVDEVLKAKEKEIMEE"
     gene            2062..3042
                     /locus_tag="PK849_02345"
     CDS             2062..3042
                     /locus_tag="PK849_02345"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HPE64993.1"
                     /translation="MSLPKVYVSRRIQDAGMRLLDGRVEYEVWEEIGPVDRRILLEKG
                     ADADGLLVTLSDRIDDELLSLCPNLKVVSNYAVGYDNIDVDAATRRGVLVTNTPDVLT
                     NATADIAFTLLLASARRMVEANEFLRSGDWVTWHPNLLLGADVSEAVLGIVGMGKIGQ
                     AVARRAKGFDMEILYVNRSPKPEIESMLGARRVSLQELLAKSDFISLHCPLSGETRGL
                     IGEKELRSMKKTAFLINTSRGPVVDQKALLKACSEGWIGGAGLDVFDKEPVPLDEPLL
                     TLKNVTTLPHLGSATIKAREGMARKAAENVLAALEGRRPADLVNPEAFKK"
     gene            complement(3099..4289)
                     /locus_tag="PK849_02350"
     CDS             complement(3099..4289)
                     /locus_tag="PK849_02350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPE64994.1"
                     /translation="MYVSTRARKMEPSATLAVTAKAKSLKREGKPVISFGAGEPDFNS
                     PPSALRFAEEAMKKGQTHYTPGTGIPELREAVCAYYKEHFGLEYKPADVVVGAGAKPL
                     LYEALGCLLDPGDEVLVFTPAWVSYVEQIRFFDGKAVLIDTSKTDFVPRIEDVRKAVT
                     PRTRAMIINTPNNPTGAVYDEQCLRDLGKLAVEKDITILYDEIYERLVYGNAVHHQIL
                     NLVPEAREHTIIINGVSKAFAMTGWRIGYALGPSSIMGYLGDLQGHITSNACSVAQWA
                     SVGALKEAEQDVVTMHKAFSKRRDLIVELMKEMPYISFTEPKGAFYVWFNIENALGKS
                     WKGQSLTDDTTFCKVLLESKYVALVPGSAFMADGNVRISYSNSEEEIREGMRRFKEFL
                     SELA"
     gene            4432..4968
                     /gene="raiA"
                     /locus_tag="PK849_02355"
     CDS             4432..4968
                     /gene="raiA"
                     /locus_tag="PK849_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPE64995.1"
                     /translation="MDIRFVTRGVEMKDELKDYMENKMSKLERFFNRIIDSQIVVSFG
                     RGMYTVEITSNANGVIMRGQENDSDMRKAFDQALKNLERQIKRHKSYLKDRAQLKTHD
                     ISFEIPVVEPELPIPPEEEIVKVKRFPLRPMSAKEATMQMDLLGHEFFLFKNAESGIV
                     NVVYKRKDGGYGLLEPSE"
     gene            5157..5498
                     /gene="hypA"
                     /locus_tag="PK849_02360"
     CDS             5157..5498
                     /gene="hypA"
                     /locus_tag="PK849_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPE64996.1"
                     /translation="MHELSLADAIVSALLKMCEDEAWGRVKKVRLRVGALRQVFPEML
                     EFAFSTVVKDTALSGALLEIEEIPLEWRCLECGNRWREDSGVCPLCGSFNRETLAGME
                     LEIHSVEVEET"
     gene            5495..6160
                     /gene="hypB"
                     /locus_tag="PK849_02365"
     CDS             5495..6160
                     /gene="hypB"
                     /locus_tag="PK849_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPE64997.1"
                     /translation="MNKIVTLQQSVMAEDEKFAAAIRDRNTRMGLLMVNIIGSPGCGK
                     TTLLEKTALHAGLSMAVIEGDLATDRDAERLARLGVPVVQINTKGGCHLEANVVEKAM
                     ESLPLDTLDVLFIENVGNLVCPAEFDLGEDFKVAVLSAPEGADKPLKYPYLFTSAKAV
                     LLTKTDLLPHLSFDMEMFAEDTRKLNPAAPVFHVDSLSGAGIDQWVGFIKLKAKEKKA
                     NVR"
     gene            6150..8168
                     /locus_tag="PK849_02370"
     CDS             6150..8168
                     /locus_tag="PK849_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPE64998.1"
                     /translation="MFAEQRPNKGLKMAEAERDLLEKLNPRQREAVAYCDGPLLVLAG
                     AGSGKTRVLTHKIAYLIAAGAAKPWDFLAVTFTNKAANEMKDRVRSLVGEGGGDMQVC
                     TFHSFGLRFLFRNRSHLPSVGLREGFAIFDRGDSRSLVKEIIESFNLDPKQFEAAALL
                     DRISTVKNDCRSPSSGQSPLLEGVYGDIFTAYNEALRRQNAVDFDDLLLLPLQLLSGD
                     EELRSREQSRLSWILVDEYQDVNRPQYLLLRKLVGDRNKIMVVGDPDQSIYGWRGADM
                     GMILNFEKDFPSAKVVVLDQNYRSSGNILGAANALIEGNSARRKKDLWTSRNMGEKIY
                     TMLGKSEYEEADFLVSEIHRLHEREGYGFGDIAVLYRINAMSRVYEQKFLENRLPYRV
                     VRGTAFYERKEVKDVLSFLRAAVNPFDRVSLSRIANIPARGLGKKSLEKVFEFLDSSV
                     PLPPEEFWSSAASRKIIDGKAGVGFQQLAEHLARILHKSDDLNDILVYILDTMGYEAV
                     LRNDDPEGWEERVENIRELRSIVPSGGDLAEMLAEAALFTDLETLNLEDGNAVNLLTL
                     HAAKGLEFPVVFLVGMEEAVFPHFKCLEDREGMEEERRLCYVGMTRAEERLYLSGGRS
                     RRLFGTTFRNGLSRFLWEIPDRFKRVDDRGEEEKTHVGFSGNRRRWGW"
     gene            8128..>8799
                     /gene="coaE"
                     /locus_tag="PK849_02375"
     CDS             8128..>8799
                     /gene="coaE"
                     /locus_tag="PK849_02375"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPE64999.1"
                     /translation="MLVSAVTGDVGAGKSTLTAFWREAGASVIDADEIVRMLWTRPEI
                     ITAAGFRWGEGIFDGKGAVLPSRVAARAFGDEKEYRWLCDLLHPLVRIEMERRTASLG
                     GWVVAEIPLLFESGPPWWVDVSVYVTAPDEFRTLRNSVRGWNEDEIPRRESFLLPSEE
                     KRRRASLVMENSGTLEEFRALAIKQADLFKRASAIVRGSVLFQKNEDARRYAEFLPLK
                     DLEFWT"
BASE COUNT         2111 a         2212 c         2481 g         1995 t
ORIGIN      
        1 accttcgcga aaaaggtctt gccaaagctg caaagaaggc cgccagaact gccgcagacg
       61 gtcgtgtttt ctcgtatatc cacacgaacg gcaaactcgg cgctttgatc gagctgaact
      121 gtgaaactga ttttgttgca aagacagatg aattccagaa ccttggacat gaactgtgca
      181 tgcacatcgc tgctgcggcg ccgcagttcc tttcctcgga ggaagtaacc gccgacgtgt
      241 tggaaagaga acgtgagatc tacaagcagc aggcccttga agaagggaaa cccgcaaata
      301 tcgtcgataa aatcgccgac ggtaagatca acaagttcta cgagctcaac tgcctgctcg
      361 agcagaattg gatccgagac ggcgacaaga agatcaagga tctcatcgta gaggttatcg
      421 caaaactcgg cgaaaacatt gtcctcagac gcttctcccg attctcgatc ggtgaatagt
      481 tttttataaa aaaaggcggg gccgaaagcc tcgccttttt catacccgaa atcttgagaa
      541 ggggaggaat tccgatgaaa ttcaacagag ttcttttaaa gctctccgga gaggttcttg
      601 cgggaaataa cggatttggc ttcgattttg acgcagtccg gcggataggc gaggagatcg
      661 ttgaggtcgc ccgctcgggt atccagattg ccatggtcgt aggagggggc aatatgcttc
      721 ggggaaaaga acttgagtcc ctcggggtag aacgttccca agccgactat atgggaatgc
      781 tggccacggt tatgaacgcc ctctgccttc aggatgttct cgaaaagttc ggtgtgccga
      841 cacgtgtaca gacagcgatc gagatgcgcc aaatggctga accgtacatt cgccgcaggg
      901 ccttgcgtca tctcgaaaaa gagcgcgtcg tcatctttgc ggcaggaaca ggctctcctt
      961 atttctctac ggacacggcc gccgcgcttc gtgcggcgga ggtgggagca gattgcctgt
     1021 tgaaagcaac gaaagtagat ggtatatata gtgcggaccc gaaaaaattt ccggatgcgg
     1081 ttctgctgca ccgcttaacc tatatggaag ctcttcagcg tcagttcgag gtaatggatg
     1141 cggccgcttt ctcgctctgt atggagaatg ccatacctat cgttgtattg aacatactgc
     1201 gtaagggcaa cctgaaagat tttctggttg acgggaagaa cacgggaacc atggtatcgg
     1261 cgtaggagag tctcgaaaga gagacgtgcg cgcgcagaaa tccagcgtat cgtcggctga
     1321 tcgcatgccg aaggagacgc cggagtgttt tttagttctg cactggatgc ttgactgttc
     1381 cgcccccatt tttcggggat ctattcttca tcgaaggagg aattccggaa tgccgaaaac
     1441 tgagctgaaa gaactgagag aaagaatgga gaaggtcatt gagcacttaa agaacgagtt
     1501 cttggctatt cggaccggac gcgctcatcc cggtctcgta agcgacatca aggtggatta
     1561 ctacggagcc cctacgcctg tcaagcaaat tgccacagtc tccattccgg aggggcgtca
     1621 aattgctatc gcccccttcg acagaagcgc tttgaaactc attgaaaagg caatcctcgc
     1681 ctcttcgctc ggagtcactc cgcagaacga cggcgaaatt attcgcgtga atcttccgga
     1741 gctcacaaga gatcgtcgta ttgaactgac aaaactggtg ggcaaatatg cggaagaggg
     1801 acgtatcgct cttcgaaacc tccggaggga ttcgaacgac gttctgaaga aaaaggaaaa
     1861 gaactcggag atcagtgaag acgacctcaa aaagtataca aaagaggttc aggatattat
     1921 cgacgagtac ataaagaagg tagacgaggt tctcaaagcg aaagagaaag agattatgga
     1981 ggaatagctt tttctattca caaccggcag gggcggagtt ttctccgccc tttttctcag
     2041 ggagttgcag gaggtgtttt cgtgtctctt ccgaaagtat atgtttcacg taggatacag
     2101 gacgccggaa tgcgcctgtt ggatgggcgt gtcgagtacg aagtctggga agaaatcggc
     2161 cccgtagacc gccgcatact gctcgaaaag ggcgcagatg cggacggttt gctggtgact
     2221 ctttcggata ggatcgacga tgaacttctg tcactctgtc cgaatttgaa agtcgtcagc
     2281 aattatgccg tagggtatga caatatcgac gtcgatgcgg ctacacggag gggcgtcttg
     2341 gtgaccaaca ctcccgatgt gctgacgaac gccaccgcag acattgcgtt tacgcttctc
     2401 ttggcgagcg cacggaggat ggtggaggca aacgagtttc tgcgttcggg cgattgggtg
     2461 acgtggcatc ccaatcttct gctgggcgcg gacgtttcag aagcagtgct gggtatcgtc
     2521 ggcatgggaa aaatcggcca ggcggtcgca cgccgggcaa aaggattcga tatggaaatc
     2581 ctctacgtca accgttctcc gaagcctgaa atcgagagca tgctcggcgc caggcgcgtc
     2641 tctctccaag aactgctggc taaaagcgac tttatctcgc ttcactgccc attgagcggc
     2701 gaaacgcgag gattgatcgg tgaaaaagag cttcgctcga tgaagaagac cgcatttctt
     2761 attaatacgt cgcgaggtcc tgttgtcgac cagaaggcgc ttttaaaagc gtgctccgaa
     2821 gggtggattg ggggagcggg actcgacgtc ttcgataagg aaccggttcc cttggacgag
     2881 ccgcttctta cactgaagaa cgtaacaacg cttccgcacc taggaagcgc aaccattaaa
     2941 gccagggaag gcatggcgag aaaagctgcg gagaacgtac tcgcggctct cgaaggacgc
     3001 cgcccggcag acctggtaaa cccggaagca ttcaaaaaat gaagagaagc ggagcgttct
     3061 gtacattacg ctccgctttc tattcccaat cggcagaact acgccagttc gctgaggaac
     3121 tccttgaaac gccgcattcc ttcgcgaatc tcctcctcgg agtttgaata cgaaatgcgc
     3181 acgttgccgt cggccatgaa tgcgctgccc ggcacaagcg ctacatactt cgactcaagc
     3241 agtaccttgc agaaggtcgt gtcatcggta agagactgtc ctttccagga ttttccaaga
     3301 gcgttctcta tattgaacca cacgtagaac gcgcccttgg gctccgtgaa cgaaatatag
     3361 ggcatctctt tcatcagttc cacgatgaga tcgcggcgct tgctgaatgc cttgtgcatc
     3421 gtgacgacgt cctgttcggc ttctttcaag gcgccgacgg aggcccactg ggcaacggaa
     3481 cacgcgttcg atgtgatgtg tccctggagg tcgcccaaat accccatgat actcgaaggg
     3541 ccaagggcgt atccgatacg ccagccggtc atcgcaaaag ccttgctcac gccgttgatg
     3601 atgatggtat gctctcgcgc ctccggaacc aggttcaaaa tctggtggtg cacagcgttc
     3661 ccgtagacaa ggcgttcgta gatctcgtcg taaaggatag taatatcctt ctcgacagcg
     3721 agtttgccga ggtctcgaag acactgttcg tcatacaccg cgccggtcgg gttgttcggc
     3781 gtattgatga tcatggcgcg cgttctcgga gtcaccgctt tcctgacatc ctcgatccgg
     3841 gggacaaaat cggttttgct cgtgtcgatg agcaccgctt tcccgtcgaa aaagcgaatc
     3901 tgctccacgt aactcaccca tgcaggcgta aaaacaagca cttcatcacc cgggtcgaga
     3961 agacagccga gcgcctcgta aagcaaaggc ttggccccgg cgcccacaac aacgtcggcc
     4021 ggtttgtact caagcccgaa atgctctttg taataggcgc atacagcttc tctcagctcg
     4081 gggattcctg ttcccggagt gtagtgcgtt tgcccctttt tcatcgcctc ttccgcgaag
     4141 cgaagagcag agggaggcga attgaaatca ggctctcctg cgccgaagga gataacgggt
     4201 ttgccctccc gcttgagact ttttgccttt gccgttacag caagggtcgc cgaaggttcc
     4261 atcttccggg ccctagtcga aacatacatt tcacagtacc tcctgaatga tatttttttg
     4321 tgtgatgtat actcattctt taaaaaaccc cgagtgaaag cctttgttgc ccttctcctt
     4381 acccggtgct agaataatac catattacag agtctgaatg gagggatttg catggacata
     4441 cgtttcgtga cccgtggcgt cgaaatgaaa gatgaactca aggattacat ggagaacaaa
     4501 atgtccaagc ttgagaggtt tttcaaccgc atcatcgaca gccagatcgt ggtgagtttc
     4561 ggacgtggta tgtacactgt cgaaattacc tccaacgcca acggggtaat aatgagaggg
     4621 caagagaacg attccgatat gaggaaagcc ttcgaccagg cgctcaaaaa ccttgaacgc
     4681 cagatcaaac gccacaagag ttatttgaag gacagagcgc agctgaaaac gcacgacatc
     4741 tcctttgaga ttcctgtcgt ggagcctgag cttcctatcc ctcccgaaga ggaaatcgtg
     4801 aaggtgaaac gtttccccct gcgtcctatg agcgcaaaag aagcgacgat gcaaatggat
     4861 cttcttgggc atgagttttt cttgttcaag aacgccgagt ccgggatagt gaacgtggtg
     4921 tacaagcgga aggacggcgg atacggcctt cttgagcctt cggagtgatt ttcagtcgca
     4981 gcgggcgccc ctcgcgcggg ggcttcggat cgcggtaact cgatctgtac cggtgaatgt
     5041 gtgtattctc cggtttttcc cccgcctgaa cgggtatggc aggtttttcc ccgtcagtaa
     5101 ccgtcgatgc gaaaaggacc cgaaagggtc ctttttttct ggaaaagagg aacggcatgc
     5161 atgaactctc tcttgccgat gcgattgtat cggccctttt aaaaatgtgc gaggacgaag
     5221 catggggcag ggtaaaaaaa gttcgccttc gggtcggcgc ccttcgccag gtttttcccg
     5281 aaatgctcga attcgcattc agtacagtgg ttaaagatac ggcgctttcc ggggcgctcc
     5341 tcgaaatcga ggagattcct ctggaatgga gatgccttga atgcgggaat cgctggcggg
     5401 aggattccgg agtctgtccg ttatgcggct ctttcaacag ggagacgctt gcagggatgg
     5461 aactggaaat tcattctgtg gaggtcgagg aaacatgaat aagatagtta cgctgcaaca
     5521 gtccgtgatg gcggaggatg agaaattcgc cgccgcgatc agggacagaa atacccgaat
     5581 gggccttctg atggtcaata tcatcggttc tcccggttgc gggaagacaa ccctgctgga
     5641 aaaaacagcg cttcatgcag gactctcgat ggctgtaatc gaaggagatc ttgccacaga
     5701 ccgcgatgcg gaacgattgg ctcgccttgg cgtacccgtc gtgcagatca acacaaaagg
     5761 cggatgccac cttgaagcca acgttgttga aaaagcgatg gaatcgcttc cgctggatac
     5821 gctggacgtt cttttcatcg agaacgtcgg caaccttgtc tgcccggcgg aattcgacct
     5881 cggtgaggat ttcaaggttg cggtgctgag cgctccggaa ggtgccgata aaccgctgaa
     5941 atacccgtat ctcttcactt ccgcgaaagc ggtgctgctg accaaaaccg atctgcttcc
     6001 ccacctctct ttcgacatgg agatgtttgc ggaagatacg cgaaaactca atcctgccgc
     6061 gcccgtgttt cacgtcgatt ctctttcggg cgccggtatc gatcagtggg tcggctttat
     6121 aaagctcaaa gcgaaggaga aaaaagcgaa tgttcgctga acagcgccca aacaaaggat
     6181 tgaagatggc agaagctgaa cgggatctgt tggaaaagct gaatccccgc cagcgggagg
     6241 ctgtggcgta ctgtgacggc cctcttcttg tgctcgccgg cgccggaagc ggaaaaacgc
     6301 gcgtcctcac gcacaagata gcctacctta ttgccgccgg agccgccaaa ccatgggact
     6361 ttcttgccgt taccttcacg aacaaagcag caaacgagat gaaagatcgc gtccgctctc
     6421 ttgttggaga agggggcggc gatatgcagg tgtgcacatt ccactctttc ggattgcgtt
     6481 tccttttccg aaacaggtcc catctgcctt ccgtcgggct tcgggaagga ttcgctatct
     6541 tcgaccgggg agacagccgc tccttggtga aggagattat agaaagtttt aaccttgatc
     6601 cgaagcagtt cgaggctgcc gcgctgcttg ataggatttc caccgtaaaa aacgactgcc
     6661 gttctccttc gtcggggcag tctcctcttc tagagggagt ctacggcgac atcttcaccg
     6721 cgtacaacga agcgctccgg cgtcagaatg cggtcgactt cgacgatctt cttttgctcc
     6781 ctttgcaact tctttcggga gacgaggagc tgcgttcacg agaacagtcg agactttcct
     6841 ggatattggt ggatgaatat caggatgtaa accggcctca atatcttctt ttacgaaagc
     6901 ttgtcggcga ccgaaacaaa ataatggtgg tcggagatcc tgatcagtcg atctacgggt
     6961 ggcgcggcgc cgatatggga atgattctga acttcgaaaa ggacttcccg tctgcgaagg
     7021 ttgtggttct tgatcaaaat taccgctctt caggaaacat cctcggagcg gccaacgcgc
     7081 tcatagaggg gaactcggcc cggcgaaaga aagatctctg gacttcgcga aacatgggag
     7141 aaaaaatcta cacgatgctg gggaagagcg agtacgaaga ggcggatttt ctggtctctg
     7201 agatacatcg tctccatgaa cgcgaggggt acggtttcgg ggatatcgca gtgctctaca
     7261 ggatcaacgc tatgagccgc gtgtacgagc agaaattcct tgagaaccgc ctcccctaca
     7321 gggtggtgcg tggaacggcc ttctacgaac ggaaagaggt caaggacgtg ctctcttttc
     7381 tgcgtgccgc cgtcaaccct ttcgaccgcg tttcgctctc ccgtatcgcg aacatacccg
     7441 cccgcggtct cggaaagaaa tctctcgaaa aggtcttcga gtttcttgat tcgtccgttc
     7501 ctctcccgcc cgaggagttt tggagctccg ctgcttcccg caagataatc gacggaaaag
     7561 cgggtgtcgg tttccagcag ctggcggagc atcttgcgcg tattcttcac aaatcggacg
     7621 atctgaacga tattcttgtg tatatcctgg acacgatggg gtacgaagca gtgctccgaa
     7681 atgacgaccc cgaagggtgg gaggagcgag tcgaaaatat ccgggaactt cggtccatcg
     7741 ttccgtcagg aggggatctc gcggaaatgc tcgccgaagc ggcgctcttc acagatcttg
     7801 aaacgctcaa tctggaagac ggcaacgccg tcaatctcct gacgctccat gcggccaagg
     7861 ggctagagtt ccccgtggtg tttctcgtcg ggatggaaga agctgtattt cctcacttca
     7921 aatgtcttga agaccgcgaa ggcatggagg aggaacgccg gctctgctac gtggggatga
     7981 cccgcgcgga agagcgtctt tatctctccg ggggccgctc gcgccgtctt ttcggcacga
     8041 cgttccgtaa cggactctcg cgttttctct gggaaatccc cgaccgcttt aaaagagtgg
     8101 acgatagagg cgaggaggag aaaacacatg ttggtttcag cggtaacagg agacgttggg
     8161 gctggtaaat cgacgctgac tgctttctgg agggaagcgg gagcgtctgt gatcgatgcg
     8221 gacgagatcg tccgcatgct ctggacacgt ccggaaatca tcacagcagc cggcttccgg
     8281 tggggagaag gcattttcga cgggaaggga gccgttctcc cttcccgagt cgccgccaga
     8341 gcattcggcg atgaaaaaga gtaccgctgg ctctgcgatc ttctgcatcc cctcgtaagg
     8401 atcgagatgg agcgcagaac ggcgtctttg ggcggatggg tcgtggcgga gattcctctt
     8461 ctttttgaaa gcgggccccc atggtgggtc gatgtaagcg tctacgtaac ggctccggat
     8521 gaattccgca cgttacggaa cagtgtgcgc ggatggaacg aagacgagat cccgcggcga
     8581 gagtcttttc ttctgccttc ggaggaaaaa cggagacgcg cttctctggt catggagaac
     8641 tctggaacgc tcgaggagtt tcgcgctctt gcgatcaaac aggctgattt gtttaaacgg
     8701 gcgagcgcga tagtgagagg ctcagttctc tttcaaaaaa acgaagacgc ccgaaggtat
     8761 gcagaatttc ttcctttgaa agaccttgaa ttttggacg
//
LOCUS       DAOPKU010000044         8754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8501_length_8754_cov_4.166801, whole genome shotgun sequence.
ACCESSION   DAOPKU010000044 DAOPKU010000000
VERSION     DAOPKU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8501_length_8754_cov_4.166801"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..250
                     /locus_tag="PK849_02380"
     CDS             80..250
                     /locus_tag="PK849_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65000.1"
                     /translation="MANLHIYRTLYAPSRLSASDAAAPGDVPDEDAEPGSAGSEPEES
                     DDAPVPADDGGK"
     gene            complement(285..2405)
                     /locus_tag="PK849_02385"
     CDS             complement(285..2405)
                     /locus_tag="PK849_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HPE65001.1"
                     /translation="MLKVKEAMPKDVGHSIARISPEDMAALGLSEGQIVEIEGKRRTP
                     VRILSCDSDLVASGGAKGVVQIDGITRDNAQASIDDKVSVHTTTHHFAGSVTLRPLTS
                     TALLEKERDAAYIRSLLEGLPVLTDDRVRTNLFGARICDFRVVDTTPAGTVIISKSTY
                     VNVEKQAEGEKKAKISYEDIGGLGPQIQRIREMIELPLRFPQVFDRLGVQPPKGVLLY
                     GPPGTGKTVIARAVASETDVYFTHISGPEVIGKFYGESEERLRNVFEDAQAHAPSIIF
                     IDEIDAIAPKREEMGGEKQVERRVVAQLLALMDGLESRGQIVVIGATNIPNTLDPALR
                     RPGRFDREISIPIPDKKGRYEILTIHTRGMPLAADVDLRRLADMTHGYVGADIEALAK
                     EAAMASLRELLPEIDMEMEEIPYDRLLAMDVRMDHFLDAFREVDPSAIREVFVEIPDV
                     SWRDIGGLEDIKEELHDAVQWPLEHADIFEKFAISPPKGIMLHGASGTGKTLLAKALA
                     HESGVNFISVKGPALMSKYVGESERAIREVFRTAKQAAPSILYFDEIESLVPVRGKDS
                     GSGASFTERVISQFLAEMSGIEELKGVVVLATTNRLDLVDPSLLSSGRFDLLLELPKP
                     DAAAREKIFEIHLRKKPLSAEIRIPTLARLTDGRSGGDIAFVCRKASMMAICDHIKGG
                     GEAEPLLEWRHFETALKELKNVLS"
     gene            complement(2523..3734)
                     /locus_tag="PK849_02390"
     CDS             complement(2523..3734)
                     /locus_tag="PK849_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPE65002.1"
                     /translation="MKFSERVLAMQSSPVRRLLPYAEEARAKGKKIYPLNIGQPDIKT
                     PESFLKAIKDFSQEVIAYATSQGNNDLRDAISRYYKEFNIPIEREDLIVTNGGSEALL
                     FAFMTMCDPGDEVLIPEPFYANYNAFAQSVNVKVVPITTKAEEGFHLPPKEQVEALVT
                     PRTRAILVANPGNPTGTIYTRDEMDMLADLALRHNLYIVADEVYREFVYDGLKYTSFG
                     HLPEIQDRVIIVDSVSKRYSACGARIGCIACKNKDFMAQVLKIAQGRLCVPTLEQVGA
                     TALYSTPKSYLDEVNKEYQSRRDTLYAALKKMPGVICSEPKGAFYVMVKLPVDDAEKF
                     IIWMLQNFDVDGETVMASAGEGFYATPGLGKQEFRLAYVLKNDELVKAMDILKVALEA
                     YPGRIEPVAAR"
     gene            3884..5092
                     /locus_tag="PK849_02395"
     CDS             3884..5092
                     /locus_tag="PK849_02395"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPE65003.1"
                     /translation="MKRMDILKSLATDNGKKMILLVMDGIGGLPGPEGKTELEAAATP
                     NMDALARKSELGLLDMVDVGITPGSGPGHLSLFGYDPMEFSIGRGILEAMGVGALVGP
                     GDVCARGNFCTRSDDDVITDRRAGRIPTEKSALVVKKLAESIKEINGVRVTFYPGKEH
                     RFVVVFSGEGLSENVADADPQQEGSPMRWARPTVSDGERMADVANAFIKKVAEVLKGE
                     APANGCLLRGFSSAPDIPLLGDVYKIKPVAVATYPMYKGLAKLVGMNVVEAGETLDKL
                     FDSVGRLWSDYDYFYVHVKYTDSRGEDGDFEGKKKVVETVDALLPRLLALSPDVLVIT
                     GDHSTPSVMSGHSWHPVPFLLASEFVRGGDSKSFDERECRIGAGGRIEAKKLLGLMLA
                     HARRLDKFGA"
     gene            5126..6157
                     /locus_tag="PK849_02400"
     CDS             5126..6157
                     /locus_tag="PK849_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE65004.1"
                     /translation="MEEQQICTIGEIRRMSKDEKFKVLGVVVKSSTRKDKNGKNYWDL
                     GIMDSEGTIEGKVWSNAQWWDRRDAPLLVAEPAESEILRDLTGKTVGLVGQVTEFRGQ
                     QQYNFNAVSLVNQEKYPPHQYVQRSPVPEESLERAFLELLEVCEGPVGDFLDFVFFKK
                     NLWERFKTCPAAVSHHHAYVGGLLEHTIAVARTAKNIVEAGISGGYEADVAVTLAGAL
                     LHDLGKMEAYRLVPAPVMTVAGTFHDHIVIGYSMFESLAREFNLDERLFLAIGHIIVS
                     HHGCREYGSPVLPATPEAMAVSAADEMDFRLFCWKHSVDQMEDGREISDFNPSAQRRF
                     WKWELNNGV"
     gene            6147..7076
                     /locus_tag="PK849_02405"
     CDS             6147..7076
                     /locus_tag="PK849_02405"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPE65005.1"
                     /translation="MGYKLHISPSDDGRRLDKTIRSIWPALPLSVVMKGIRTGAVRLD
                     GKKAACDVRITAGQELYVPWEGPAEREEGFRYRRTLPILFEDERLLAVNKPENLLVQP
                     DRKDQDNVLDRVKYMRSLEGAEERAYAVHRLDRNTTGVLLVALSGVSLRILQDAFRAR
                     EVRKTYLAVVSGVPPAKGEIDAPLLKDPASNTVKVDPSGKEALTRYRLLAAGGDVSLV
                     EIDLVTGRSHQARVHMASIGHSILGDIRYGSENVNAKWRKRGIRRPLLHSYQVVFGSL
                     LGELESLSGMRIAAPLPEDMGELLRSRGWKPPV"
     gene            7268..7441
                     /locus_tag="PK849_02410"
     CDS             7268..7441
                     /locus_tag="PK849_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65006.1"
                     /translation="MEAEFQKIEQDGVVLWVPSFMEFKNNEILIVQRGFLWSAYLMLV
                     GVLSESEESCCNS"
     gene            7635..>8754
                     /locus_tag="PK849_02415"
     CDS             7635..>8754
                     /locus_tag="PK849_02415"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPE65007.1"
                     /translation="MSVVKRTSSNVLLDTALKNFYAAAEEMGLEEGLIDILSHSERKT
                     SVSIPVEMDDGSVKVFDGYRVLHSSAIGPGKGGVRFHPEVNVEECEALAMMMTWKCSL
                     AGIPYGGGKGGVSCEPLELSKKEKERISRTWAARMAPVIGAWTDVPAPDVNTGGPEMV
                     WFMDTISKMKNQLEPAIFTGKPIPLWGSKGRTAATGYGVATCALELLKNLGKDPKAIS
                     VSIQGFGNVGTYAALTMVNAGSKVVAISDITGTYFAEKGLDIKKAIDHVNNHPKHLLE
                     GFTCDGCQKLDLPEALFVNCDILIPAALEGVINGKNADKVKAKYIVEAANGPVTPEGD
                     AILDAKGVLVVPDFLANSGGVIGSYFEWAQDLGGFFWSE"
BASE COUNT         1890 a         2310 c         2606 g         1948 t
ORIGIN      
        1 cggtcggaag gacgtgccgc acgattagtg gattgaatcc gtccgtttcg aggcaaccaa
       61 cgactatgta caaaaggtga tggcgaacct gcatatctat cgcacgctgt atgcgccttc
      121 acgtctctct gcctcggacg cggccgcgcc gggagacgtt cccgacgagg atgcggagcc
      181 ggggagcgcc ggatcggagc cggaggagag cgacgacgcc cccgttcctg ctgatgacgg
      241 aggaaaataa agaagggcgg accggataac ggtccgccct tcttctatga taaaacgttc
      301 tttagctctt tgagcgcggt ttcgaaatgc cgccattcga gaagaggttc ggcttcgccg
      361 ccgcctttga tgtgatcaca tatcgccatc atcgacgcct tgcggcagac gaaggcgatg
      421 tcgcctcctg aacgcccgtc ggtcagcctc gccagcgtcg gaatacgaat ctccgcagac
      481 agcggttttt ttcgaagatg aatctcgaag atcttctccc tggcagcagc gtcgggcttc
      541 ggtagttcaa ggaggaggtc gaatctgccg ctggagagaa gcgaaggatc cacaagatcc
      601 agacggttcg ttgttgcaag gaccaccacg cctttcagct cttctatgcc gctcatctct
      661 gcaaggaact gactgataac tcgctctgtg aagctcgctc cgctgccgct gtccttcccc
      721 cggaccggga caagagattc aatctcgtcg aagtagagta tggaaggagc agcctgcttc
      781 gcggtacgga agacctcgcg gatggcgcgt tcgctctcgc cgacgtattt cgacatgagc
      841 gccggtccct tcacggagat gaagttcacg ccgctctcat gggcgagcgc ctttgcgaga
      901 agcgttttgc ccgttcccga agctccatga agcatgatac ctttgggagg cgagatggcg
      961 aatttctcaa agatgtcggc gtgttccaga ggccactgga ccgcgtcgtg cagctcttct
     1021 ttaatatctt ccaaaccgcc gatatcgcgc cacgatacat cggggatctc aacgaaaacc
     1081 tcccgtatcg ccgaaggatc gacctccctg aacgcgtcga ggaagtggtc catccgtacg
     1141 tccatggcaa gaaggcggtc gtagggaatc tcttccatct ccatgtcgat ctcggggagc
     1201 agttcgcgaa ggctggccat cgcggcctcc ttggcgagcg cctcgatatc cgccccgacg
     1261 tatccgtgcg tcatgtctgc aagacggcga agatccacgt cggccgccag cggcatcccg
     1321 cgcgtatgaa tggtgagaat ctcataccgg cccttcttgt cggggatagg tatggagatc
     1381 tctctgtcga agcggcccgg acgcctgagc gccgggtcga gagtgttcgg tatgttggtg
     1441 gcgccgatga ccacgatctg accgcgggac tccagcccgt ccatgagcgc caggagctgg
     1501 gcgacaaccc tgcgctcgac ctgtttttct ccgcccatct cctcacgctt cggggcgatg
     1561 gcgtcgattt cgtcgatgaa gatgatcgag ggcgcatggg cctgcgcgtc ttcgaatacg
     1621 ttgcgcagcc gttcttcgct ttcaccgtag aacttgccga tgacctccgg gcccgaaatg
     1681 tgggtgaaat aaacgtccgt ctccgaggct acagccctcg cgataacggt ctttccggta
     1741 cccggagggc cgtagagcag tacgcccttc ggtggttgaa cgccaaggcg gtcgaagacc
     1801 tgagggaagc gcagcggcag ttcgatcatc tcgcggattc tttggatctg aggtccaagc
     1861 cccccgatgt cctcgtagga tatctttgcc ttcttctcgc cctccgcctg tttttccacg
     1921 ttcacgtagg tggacttgct gatgatcact gttccggcag gcgtcgtgtc gacgacgcgg
     1981 aagtcacaga tacgcgcgcc gaagagattg gtacgcaccc ggtcgtcggt aagaacggga
     2041 aggccttcaa gaagggaacg gatatacgcg gcgtcgcgct ctttctccag cagcgccgta
     2101 gaggtaagcg gacggagcgt aacgcttcct gcgaagtggt gcgtcgtcgt atggacggac
     2161 accttgtcgt cgatactcgc ctgcgcgttg tcccttgtga taccgtcaat ctggaccacg
     2221 cctttcgcgc ccccggaggc gacaaggtcg gaatcgcatg agagaatccg gacgggagtg
     2281 cggcgtttcc cttctatttc gactatctgg ccttccgaaa ggcccagcgc ggccatgtcc
     2341 tcgggggaga tgcgggcaat actgtgtccg acatccttag gcatagcttc cttgactttc
     2401 agcatgtgta acttcctctc ttcattgaat gttctgcgga aaacataata acataccctg
     2461 cggccgccgt tcatccggac cgcagggtat ggaagggcgg agaatccgtg tttacggaag
     2521 aatcagcggg cggctaccgg ctcgatacgc cccgggtagg cttcaagagc gaccttgaga
     2581 atatccattg ccttaacaag ctcatcgttc tttaagacgt acgcaagacg gaactcctgc
     2641 tttcccagcc cgggagtcgc gtagaatccc tcgccggcgg aagccatgac ggtttcgccg
     2701 tcaacgtcga agttctgaag catccagatg atgaacttct ccgcgtcgtc gacggggagc
     2761 ttcaccatga catagaaggc gcccttcggt tccgagcaga tcactccggg catcttcttc
     2821 agcgcggcgt agagcgtgtc gcggcgggac tggtattctt tgttcacttc atcaaggtac
     2881 gattttggcg tactgtacag cgctgtagcg ccgacctgtt ccagcgtggg aacgcagagg
     2941 cggccctgcg cgatcttgag aacctgcgcc atgaagtcct tgttcttgca cgcgatgcac
     3001 ccgatgcggg cgccgcaggc gctgtagcgc ttcgaaacag aatcgacgat gatgactctg
     3061 tcctgtattt ccggcaggtg gccgaagctt gtgtatttca gtccgtcgta cacgaactcc
     3121 cggtagactt cgtccgcgac tatgtagaga ttgtgtctga gcgcaaggtc cgcgagcata
     3181 tccatctcgt cgcgggtata aatcgttccg gtcggatttc cagggttcgc cacaaggatt
     3241 gcccgtgtcc gcggcgtaac gagcgcttcg acctgctcct tggggggaag atggaagcct
     3301 tcttcggctt tggtcgtaat tgggacgact ttgacgttca cgctctgtgc aaacgcgtta
     3361 taattggcgt agaatggttc agggatgagc acttcgtctc cggggtcgca catcgtcata
     3421 aacgcaaaaa gaagagcttc gctgccgccg ttggtgacaa taagatcttc tctctcgatt
     3481 gggatgttaa actctttgta gtagcggctt attgcatctc tcagatcgtt gttcccctgg
     3541 gaggttgcgt acgcaataac ctcctgcgaa aagtccttga tagccttgag gaacgattcg
     3601 ggtgttttga tgtccggctg cccgatgttg agaggataga tcttcttccc cttcgctcgc
     3661 gcctcctccg cgtacggcag tagtctgcgc accggagacg attgcatcgc caggactctt
     3721 tcggaaaact tcaccttgat gacccccttg aattgaaatt aagtggatgg accgctgaag
     3781 aaaatgataa cataaaatgc agaggttttt gcagaaaaaa acgagcactc gagacaaatc
     3841 gatccattag tgaaatgaaa acttttcgaa ggtggcgaga aatatgaaaa gaatggatat
     3901 attgaagagc ctggcgactg acaacggcaa gaagatgatt cttcttgtca tggacggtat
     3961 aggaggtctt cccggccccg aaggaaaaac ggaactcgaa gcagcggcga cgccgaatat
     4021 ggacgccttg gcgagaaaga gcgaactcgg ccttctggac atggtcgatg tcggcataac
     4081 tccgggaagc gggcccgggc atctctccct cttcgggtac gacccgatgg agttctccat
     4141 agggcgagga attctcgaag ccatgggcgt tggcgcgctt gtaggtccgg gagacgtatg
     4201 cgcgcggggg aacttttgca cccgttccga cgacgatgtg atcaccgaca ggcgcgcggg
     4261 gcgcatcccc accgagaaga gtgcgcttgt cgtgaagaaa cttgctgaat cgatcaagga
     4321 gattaacgga gtccgtgtaa ctttttaccc gggaaaggaa catcgtttcg tcgtcgtctt
     4381 ctcgggagag gggttgagcg agaacgtggc cgacgccgat cctcagcagg aaggcagtcc
     4441 gatgcgctgg gcgcggccga cggtttcaga cggcgaacgg atggcggacg ttgcaaacgc
     4501 cttcataaag aaagtcgcgg aggtgctgaa aggcgaagct ccggcgaacg gctgtcttct
     4561 gcgcggattt tcatccgctc ctgatatccc gcttttggga gacgtttata aaatcaagcc
     4621 tgtggcggtt gcaacctacc ctatgtacaa ggggctcgcc aaactggtcg ggatgaacgt
     4681 cgtcgaagcg ggcgagacgc ttgataagct tttcgacagc gtggggcgtc tttggagcga
     4741 ctacgactac ttctacgtcc acgtgaaata tacagatagt cgcggcgagg acggcgattt
     4801 tgaaggaaaa aagaaagttg tggaaacagt ggacgcgctc ctgccgcgtt tgcttgcgct
     4861 ctcgcccgac gttcttgtca ttaccggaga ccacagcacg ccaagcgtga tgagcggcca
     4921 ttcctggcat ccggtcccct tcctgctcgc aagcgagttt gtccgcggag gcgactcgaa
     4981 gtcgtttgac gagcgcgaat gccgtatcgg cgcaggcggg cgcatagagg cgaaaaaact
     5041 tctcggcctg atgctggcgc acgcccgcag gcttgataaa ttcggcgcat agcggcgccg
     5101 acgtatgcgt acatgaggtg attgagtgga agaacagcag atttgcacga tcggcgaaat
     5161 acggcggatg tcaaaggacg agaagttcaa ggtgcttggc gtcgttgtga aatcgtcgac
     5221 caggaaagat aagaacggga agaactactg ggatctcggt atcatggatt ctgaagggac
     5281 gattgaggga aaggtgtggt cgaacgcgca gtggtgggac aggagagacg ctcccctgct
     5341 cgtcgcggaa ccggctgaat cggagatttt acgcgatctt acgggaaaga ctgttggtct
     5401 tgtcggtcag gttacggagt tcagaggaca gcagcagtac aatttcaatg cggtttcact
     5461 tgtcaatcag gaaaaatacc cgccgcatca gtacgtgcag cgttctcccg tgccggagga
     5521 gagccttgaa cgcgcattcc tggaactgct ggaagtgtgc gaaggacccg tcggtgattt
     5581 tcttgatttc gtctttttca agaaaaacct gtgggagcgt ttcaagacat gcccggccgc
     5641 agtttctcac catcacgcgt acgtcggcgg tcttctggag catacgatag cggttgcgcg
     5701 cacggcgaaa aatatcgtcg aagcgggaat atccggcggg tacgaggcgg acgttgccgt
     5761 cacattggcg ggagcgctgc tccatgatct tggcaagatg gaggcgtacc gccttgttcc
     5821 tgcgcctgta atgaccgtgg cggggacctt tcacgatcat attgtgattg ggtacagcat
     5881 gtttgaatcc ctcgcccgtg aattcaacct ggatgaacgg ctttttctgg ccatagggca
     5941 tatcattgtc agccatcacg gatgcaggga gtacggttct cctgtgcttc ccgcgactcc
     6001 ggaagccatg gcggtgagcg cggccgacga gatggatttc aggctcttct gctggaagca
     6061 ttctgtcgat cagatggaag atggacggga aatatccgat tttaatcctt ccgcccaaag
     6121 acgtttctgg aaatgggagc tgaacaatgg ggtataaact gcatatttca ccttctgatg
     6181 acgggcgtcg cctggacaag acgatccggt cgatctggcc tgcgttgccg ctcagcgttg
     6241 taatgaaagg catccgtaca ggcgctgtcc ggctggacgg gaaaaaggcg gcgtgcgacg
     6301 tcaggataac ggcggggcag gagctgtatg tcccttggga agggcctgcc gaaagggaag
     6361 agggcttccg ctatcgccgc acacttccta ttctgttcga ggacgagcgc ttgctggctg
     6421 tgaataagcc ggaaaacctg cttgtccagc cggaccggaa ggatcaggac aatgtgctcg
     6481 acagggtgaa gtacatgcgg agtcttgaag gggccgagga gagagcctac gcagtgcacc
     6541 ggctggacag gaacacgacg ggagtgcttc tggtggcgct ctccggggtc tctctccgca
     6601 tactccagga tgccttccgg gctcgtgaag ttcgaaagac gtatctcgcc gtagtttcag
     6661 gggttcctcc tgcaaaggga gagatcgacg cccctttgct caaggatccc gcttccaaca
     6721 ccgttaaggt ggatccttcg gggaaggagg cgctcactcg ctacagactt cttgcagccg
     6781 gaggggacgt ttctctcgtc gaaatcgacc tcgtgacggg gcgctctcac caggcccggg
     6841 tgcacatggc gtcgataggt cattcgattc tcggagatat tcgctacggc agcgaaaatg
     6901 tcaatgcgaa gtggaggaag agggggattc gccgccctct tctgcatagt tatcaggttg
     6961 tcttcgggag ccttctggga gaacttgaat cgctttcagg catgcggata gctgctcctc
     7021 ttccggagga tatgggggag cttctacgtt cgaggggctg gaaacctccg gtataacccg
     7081 gttctacccg cgaaggacaa acttcgtcag ggaaggaggg aagagcatgg aacatgtaaa
     7141 aatcgttgcg gaagagaaga ttctggaaaa gctgcggaag aggggagaag tctatgcaat
     7201 ccggcggaca ctcggtgtcg gctgaaatgc caggagagtc gtccgtatag aggacgggaa
     7261 gcctccggtg gaggcggagt ttcaaaaaat tgagcaggat ggcgttgtcc tctgggttcc
     7321 gtcattcatg gagttcaaga acaacgaaat tctcattgta cagcgcggat ttctctggtc
     7381 tgcatatctg atgcttgtcg gggtgctcag tgaatcggaa gaatcgtgct gtaattcgtg
     7441 agattctgag aacccaaacg ctgcaagcct atggtacttc gtttctccgt taaagagatt
     7501 ttgtgttttg tggcgggtat ttaggattat tttgatgtat ttcaatttct cgcttgttct
     7561 ttggaaaaag agtgctatga tagttactaa ggtctcaaac ggctcaggcc gtaaatatac
     7621 aggaggtgtg tttcatgtct gttgtaaagc gtacttcctc gaacgtgctt cttgacaccg
     7681 cactcaaaaa tttctatgcc gcggcggagg agatggggct tgaagagggc cttatcgata
     7741 ttctcagcca ttccgaacgc aagaccagcg tgtccatccc tgtcgagatg gacgacggat
     7801 ctgtgaaggt cttcgacggc taccgggtgc tccattcttc cgcgataggc cccggcaagg
     7861 gcggcgtccg tttccacccg gaagtgaacg ttgaagagtg cgaagcgctt gccatgatga
     7921 tgacatggaa gtgttcgctg gccggtatcc cttacggcgg cggcaagggc ggagtttcct
     7981 gcgagcctct ggaactctcg aagaaggaaa aagaacgtat ttcccgcaca tgggctgccc
     8041 gcatggctcc cgtgatcggc gcatggaccg acgttcccgc tcctgacgtg aacaccggcg
     8101 gcccggaaat ggtctggttt atggatacca tcagcaagat gaagaaccag cttgaacccg
     8161 ccatcttcac aggcaaaccc attccgctgt ggggttccaa aggacgcacc gcggcgaccg
     8221 gttacggcgt tgcaacctgc gctcttgaac tgctgaagaa cctcggcaaa gacccgaaag
     8281 caatctctgt ctcgatccag ggcttcggca acgtaggaac ctacgcggcc cttactatgg
     8341 tgaacgccgg ttccaaagtt gtcgccatca gcgatattac cggaacttac ttcgcagaga
     8401 aggggcttga cataaagaaa gctatcgacc atgtgaacaa ccatcccaag cacctgcttg
     8461 aaggcttcac ctgcgacggc tgccagaagc tcgaccttcc cgaagctctc tttgtcaact
     8521 gcgatattct cattcccgca gctcttgaag gcgtcatcaa cggaaagaat gctgacaaag
     8581 tcaaagcaaa gtacatcgtt gaggctgcaa acggccccgt gactccggaa ggcgacgcta
     8641 ttcttgacgc aaagggcgtt ctcgttgtcc cggacttcct cgccaactcc ggcggcgtta
     8701 tcggctctta cttcgagtgg gcacaggatc tcggcggttt cttctggtcg gagg
//
LOCUS       DAOPKU010000045         8749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8507_length_8749_cov_5.089372, whole genome shotgun sequence.
ACCESSION   DAOPKU010000045 DAOPKU010000000
VERSION     DAOPKU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8507_length_8749_cov_5.089372"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PK849_02420"
     CDS             complement(<1..774)
                     /locus_tag="PK849_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HPE65008.1"
                     /translation="MSELEKSQARSKSETLSVLLGAAFLMATSAIGPGFLTQTVSFTN
                     ELKAVFAFAILLSILIDIVVQLNIWRVLAVSGLRGQDVANKVLPGLGYFLAFAVALGG
                     LAFNVGNVGGAALGFQVLFGADQMVGAVISAGIAITIFLWKDLGKAMDKFTQILGFLM
                     LALVFYVVFVTKPPVMETVHEMVRPFTSLAILKEYKWLVVLTLVGGTVGGYISFSGAH
                     RIIDAGIVGKENIGKISKGSINGIAITGVMRYLLFLAFLG"
     gene            940..1719
                     /locus_tag="PK849_02425"
     CDS             940..1719
                     /locus_tag="PK849_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPE65009.1"
                     /translation="MEFLSSFTGLDLSLWSWVVVLSAGAGVGIAKTAVPGSGILVVAL
                     MAMTLPSRLSVGVMLPMLIFGDLFAVGKFWMHTDKRRLLTLLLFALPGLGVGYMVLRF
                     ASDDQLRPIIGGVVLAMLILHQVSRVLKQRRGAAAAADGKTSPLLTAVFGFFAGVGTG
                     MANASGPVMSLYLLIAGLPKLPFIAVTAWFFFIMNLLKVPLFFSLGLITMQSLRINML
                     ALPSIAAGALLGYWIVKRISQERFNQIVLTLAFVAALRLLT"
     gene            complement(1943..2284)
                     /locus_tag="PK849_02430"
     CDS             complement(1943..2284)
                     /locus_tag="PK849_02430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPE65010.1"
                     /translation="MKNDSENLVQYGVYWTDLDPTQGAEMNKTRPCVVVSPPEMNMHL
                     LTVLVAPVTSRQRESYPTRVHFSIGDVSGWIVLDQLRAIDRKRLRRKIGCLDGETALL
                     VKNVIREMLVD"
     gene            complement(2271..2525)
                     /locus_tag="PK849_02435"
     CDS             complement(2271..2525)
                     /locus_tag="PK849_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPE65011.1"
                     /translation="MEVNVVKVGNSKGIRIPAPLLKQLGIGERVILEVEGDALLVRPV
                     RAPRQGWAAAFGRMRRSGDDVLLMDDALDDDMLEAWDEKR"
     regulatory      2883..3001
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3245..5989
                     /locus_tag="PK849_02440"
     CDS             3245..5989
                     /locus_tag="PK849_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE65012.1"
                     /translation="MIQKVFFVNGIERNIIVDEETTLAEVLREQLGLTGTKVGCGQGH
                     CGSCNVLMDGKVTRTCVYKMSRLPEGTKIVTIEGIGTPEKLHPLQKAWIKHGAAQCGF
                     CTPGFIVSAKGLLDTNPNPTREEVRDWFQLNRNACRCTGYIPIVDAVMDAAKVLRGEM
                     SEKELEFKMPADGRIWGSKYPRPTAVAKVTGTLDYGADLILKMPKKTLHAALVQATVS
                     HANIKGIDTSEAEKMPGVYKIVTHKDIKGKNRITGLITFPTNKGDGWDRPILCDEKIF
                     QYGDAVAIVCADSYKNAVAAAEKVKLDLEVLPAYMSAPEAMTDDAIEIHPGTPNVYFT
                     QKIRKGEETAPIFSRSDVVTVEDDFYVGRQPHMPVEPDVGFAYTDHEGRLVIHSKSIG
                     VHLHLAMIAPGLGVEPEKLVLVTNPAGGTFGYKFSPTMEALVGAAALATGHPVALKYT
                     WKQQQQYTGKRSPFFVNMKFAADKEGKLLAMETDFSVDHGPYSEFGDLLTLRGIQFMG
                     AGYDIPNIRGEGRTVCTNHAWGSAFRAYGSPQSLFSSEVLMDELAEKLGMDPLEIRYK
                     NAYRPGSTNPTGQAPESYSLPKMLEALRPKYELAKKRAAEGSTTRFKKGVGLSVGVYG
                     CGLDGPDGSEARLDMNPDGTITVCTAWEDHGQGADAGAIGTAHEALRPLGISPDKLKF
                     TWPNTAKCPNSGPAGGSRSQVMTGNAIRVACETLLRETAKPKTGFLKRDGGFMTYNEL
                     VAAGKPTSFTGKWSAVEGTACNEDGQGKPFVIYMYGVFMAEITVDTETGKTSVDRMTL
                     MCDCGKINNRLVVDGQNWGGMAQGIGLALTEDFEDIEKHSTMPGAGFPYIKDIPDDME
                     IIYFEEHREDGPHGAAGIGELPLSSPHASIINGIYNATGVRITRLPAYPEKVLAGLKK
                     "
     gene            6047..8353
                     /locus_tag="PK849_02445"
     CDS             6047..8353
                     /locus_tag="PK849_02445"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPE65013.1"
                     /translation="MEKARLLEFESKCVQEEPPYCQAACPLHLDGRALCAFVREGRID
                     DARKLIERTLPLPEALARICDAPCKEACLRRELGGAIELGALERACVEQGPLRRAPLL
                     IPKNGKRVAVVGGGLSALCAASEGIRKGYSVAVFCAEDQAAALTALSPLITDDIARRE
                     LAWLEKGGVLFLPLEDLPPLSELSVRFDAAYAGLDDPFAACATAEATASVNPVSLETS
                     VRGLFAGGAAPSFILRAADGRRGMKSAERFLQGASLFSSREKEGPYATRLFTSTEGMA
                     SAEPEAVPQEGYSPEEAIREAKRCILCECMECVKHCAYMQHYKGYPKKFAREIYNNLS
                     VIQGTRLANGMINSCTLCGRCERICPNGFSMADLCREARREMLRQEKMPPSAHDFALE
                     DMRFNNSPRATLLRHEPGKETSAYLFLPGCRLAGCTPEQTTALYAFLRDRLEGGVGFW
                     LRCCGAPADWAGRDEELHREAAKITEEWESMGSPVVVAACTSCLQTFRKATPSIKAVS
                     LWEILEGTELPEGALSFERTLAVADPCTAAQAPEIRDAVRNVASRAGVRFEEPLLSGE
                     FSFCCGFGGLQESANPTLAALSAKTRGEESSLDFLVYCAMCRNLFAQAGKPAVHLLDI
                     LFPQGDEDPAGRPAIGYSEQQENRVGLVRALRASLWKEEDIVNEPHESIRLVIPPDVE
                     KTLAKRRILADTVRRAIWSAERSGRKMKQINGALVASLRPASVTYWVEYAPKGDGSFE
                     VRNGWSHRMFVAGAAENLVPDASGGDGA"
     gene            8350..8565
                     /locus_tag="PK849_02450"
     CDS             8350..8565
                     /locus_tag="PK849_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPE65014.1"
                     /translation="MKELIKSFEKYTKTECGACGRFLELQKTEITYLGSVFTLDLPVC
                     PSCGTVFVPEELATGKMLEVEKILEDK"
     gene            8565..>8749
                     /locus_tag="PK849_02455"
     CDS             8565..>8749
                     /locus_tag="PK849_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65015.1"
                     /translation="MDVFDLDDLQMEMLSLEGKGYHCSQILILLALRQTGGERNDSLI
                     RSMGGLSLGLGHSNGTCG"
BASE COUNT         1793 a         2756 c         2600 g         1600 t
ORIGIN      
        1 gccgaggaag gcgaggaaaa gcagatagcg cataacgccg gtgatggcga tgccattgat
       61 ggagcctttg ctgatcttgc cgatattctc cttgccgacg attccggcgt cgatgatccg
      121 gtgcgcgccg gagaaggaga tgtagccgcc gaccgtgccg ccgacgaggg tgagaacaac
      181 gagccacttg tactccttca ggatagccag gctggtgaag ggacggacca tctcatgcac
      241 cgtctccata actggaggct tcgtgacgaa aacgacgtag aagacaaggg cgagcatcaa
      301 aaagccgagg atctgcgtga acttgtccat tgccttgccg aggtctttcc agaggaagat
      361 ggtgatcgcg atgcccgcgc tgataactgc gccgaccatc tggtcggctc cgaagagcac
      421 ctggaagccg agcgccgcgc cgccgacgtt ccccacattg aaggccagcc ctccgagggc
      481 gaccgcaaag gcgaggaagt acccgagacc ggggagcacc ttgttggcga cgtcctgccc
      541 tcttaaaccg gagacggcga gaacgcgcca gatgttcagc tgcaccacga tatcgatgag
      601 gatggagaga aggatcgcaa atgcaaatac cgctttgagt tcgttggtga aggataccgt
      661 ctgggtcagg aatcccgggc cgatggccga ggtcgccatg agaaacgccg ccccgagaag
      721 gacgctgagc gtttcactct tgcttcgcgc ttgactcttt tccagttccg acataatgat
      781 acctcccgat tataatgatg gtgcattcct tcttgcactg caagcattat acccgatttt
      841 cccctgcgtt ttgcaaggga tagcctgtag tttttctgaa tacaaaagac atgatgtata
      901 atggcggcac tgcgcagtca ttgaaaggtg gtatcctcaa tggagtttct ctcttctttt
      961 acggggctcg acctctccct gtggtcgtgg gttgtcgtcc tcagtgcggg cgcgggcgtc
     1021 ggcatcgcaa agacagccgt tccgggcagc ggcatcctcg tcgtcgcgct gatggcgatg
     1081 acgctcccct cgcgtctctc cgtcggcgtg atgctcccga tgctgatttt cggcgacctc
     1141 ttcgccgtgg gaaaattctg gatgcacacc gacaaaaggc gtcttctgac gctgctgctc
     1201 ttcgctcttc cggggctcgg cgtggggtac atggtgctgc gctttgcgag cgacgaccag
     1261 ttgcggccca tcatcggagg cgtggtgctc gcaatgctca tcctgcacca agtctcgcgc
     1321 gtcctgaaac aacggcgcgg ggcggctgca gcggccgacg ggaaaacatc tccgctgctc
     1381 accgcagttt tcggcttctt cgccggagtc ggaacgggaa tggcgaacgc ttccggcccg
     1441 gtaatgagcc tctacctgct tatcgcagga ctgccgaaac ttcccttcat cgccgtcacc
     1501 gcgtggttct ttttcatcat gaacctgctg aaggttccgc tcttcttcag cctggggctc
     1561 atcaccatgc agagcctcag gatcaacatg ctcgccctcc cctccatagc ggcgggggcg
     1621 ctgctgggat actggatagt caagcgcatc tcccaggagc gcttcaacca gatcgtcctc
     1681 acgctcgcct tcgtcgcggc gctgaggctg ctgacgtagc gagctatctt ccggaggaag
     1741 ccgccggagg ccagagcaaa aaaaccccgg agggttgcgg cgtccccctg cgctacctgg
     1801 gttttcactc ggtctacgtc ttttgcgtag gcgcgctcct catcgggaag gaaatgcccg
     1861 gccacttccc cgggcatttt aaaaccgggg agtattgggc ggacgaatcc cttcgcaaag
     1921 agcccctgca acggcacgct gctcagtcca cgagcatctc ccggatgaca ttcttcacca
     1981 gaagagccgt ctctccgtcc agacatccta tctttcgacg tagcctcttc ctgtctatcg
     2041 ccctgagctg atcgagaacg atccaacctg aaacgtcgcc tatcgaaaaa tgaacgcggg
     2101 taggatagct ttcacgttgt ctgcttgtta ccggcgcaac cagaacggtc agcagatgca
     2161 tgttcatttc cggcggagag acgacaacgc acgggcgagt cttattcatc tccgctccct
     2221 gcgtcgggtc gagatcggtc cagtacacgc cgtactgaac caagttctcg ctatcgtttt
     2281 tcatcccacg cctccagcat atcgtcgtcg agcgcgtcat ccatcagcaa aacgtcgtct
     2341 ccgctccggc gcatcctccc gaaagcggcc gcccaccctt gcctcggggc tctgaccgga
     2401 cgtacgagta acgcatcccc ctccacctcc agaatcaccc gctcccctat accgagctgc
     2461 ttcagaagag gggccggaat ccgtatccct tttgaattac cgacttttac aacattcact
     2521 tccacagggt gcacctcctc ttcacggaac aaaaagtact tacttcgtat ccacattcta
     2581 ccatacagac accacctcga cacgaccacc tccacacaac gcgtcgaatt tgcgttcatc
     2641 ggcggaggga actctgaaga actctgtata ccgcgtattt ccggtaccgc tgcagcgagg
     2701 ttcttccgcc ccgtctgttc aaaagaacgt gggaaacctt tgaaaagcgc cgcacaaaat
     2761 gcaaccatgt tgcaaaatca aaacattttc ccgaaatttc catcctgatg ttgcccttga
     2821 atttatgatg ctctataatg agtatcgaag gtacattttt caaaaaaaga cgactgtttc
     2881 agtcgagata aaacctccga gtcgcaggcc ctacaggaac aatcccacgg actgcgaccg
     2941 ttagggtgca tctggaaacg ggtgcgcctc ccctttggca aggaggattt ccaaaaacgg
     3001 catgcgccgt cctctttgca cggaggacgg cgtttttgta ttcttgtttc acgactcatc
     3061 gatggaaggg ggagtgcccg ggcaacggta ctgcgttctc ctcttcactg aggaaaactg
     3121 gcggaagagt gtggtttgat acggagaagg acggcggctt ccggaccgcg ttgcttgccg
     3181 gcgccccggt tcgcggcgcc gccgtcccga cccctgccga gattcggaag gagctggaaa
     3241 caggatgatt caaaaggtct tttttgtcaa cggcattgag cggaacatca tcgtcgacga
     3301 ggaaacaacg ctcgccgaag tgctccgcga gcagctcggg ctcacgggaa ccaaggtagg
     3361 atgcggccag ggacactgcg ggtcgtgcaa cgtgctgatg gacggcaagg tgacccgcac
     3421 gtgcgtttac aagatgtccc gcctccccga gggcacgaag atcgtcacga tcgaggggat
     3481 cggcacgccg gagaagctcc acccgctcca gaaggcgtgg ataaagcacg gcgcggccca
     3541 gtgcggcttc tgcacgccgg gcttcatcgt ctccgcgaaa gggctgcttg acacgaatcc
     3601 gaaccctacc cgcgaagagg ttcgcgactg gttccagctc aaccggaacg catgccgctg
     3661 caccgggtac attcccatcg tcgatgccgt gatggacgcc gcaaaggtcc tccggggcga
     3721 gatgagcgag aaggagctcg agttcaaaat gcctgcggac ggacggatct gggggagcaa
     3781 gtaccctcgc ccgaccgccg tcgcgaaggt gaccggaacg ctcgactacg gcgcggatct
     3841 catccttaag atgcccaaaa agacgctgca cgcggcgctg gtgcaggcaa ccgtctccca
     3901 cgcgaacatc aagggaatcg acacctcgga agccgagaag atgcccggag tctacaagat
     3961 cgtcacccat aaggacatca agggaaagaa ccgcattacc ggtctgatca ccttcccgac
     4021 aaacaaggga gacgggtggg accgcccgat cctctgcgac gagaagatct tccagtacgg
     4081 cgacgccgtc gccatcgtct gcgcggacag ctacaagaac gccgtcgcgg cggccgaaaa
     4141 ggtgaagctc gacctcgaag tcctgccggc gtacatgagc gcgcccgagg cgatgaccga
     4201 cgacgccatc gagatccacc ccggaacgcc gaacgtctac ttcacgcaga agatcaggaa
     4261 gggcgaagag actgctccca tcttctcacg ctccgatgtg gtcacggtcg aagacgattt
     4321 ctacgtcggc cgtcaaccgc acatgcccgt cgagcccgac gtcggcttcg cgtacaccga
     4381 tcacgaggga cgccttgtca tccactccaa gtccatcggc gtccacctgc acctcgccat
     4441 gatcgccccc ggactcggcg tcgagcccga gaagctggtt cttgtgacaa accccgccgg
     4501 aggcaccttc ggctacaagt tcagcccgac catggaggcg ctcgtcggcg ctgcggccct
     4561 cgccacggga cacccggtcg cgctgaagta cacgtggaaa cagcagcagc agtacaccgg
     4621 caaacgctcc cccttcttcg tcaacatgaa gttcgccgcc gataaagagg gcaaactgct
     4681 cgccatggag acggatttct cggtcgatca cggcccctac tccgagttcg gcgacctcct
     4741 cacgctccgc ggtatccagt tcatgggcgc cgggtacgac atcccgaaca tccggggcga
     4801 gggacggaca gtctgcacga accacgcgtg gggctccgcc ttccgcgcgt acggatcgcc
     4861 gcagtcgctc ttctcctccg aggtgctgat ggacgagctg gcggagaagc tgggaatgga
     4921 cccgctggag atccgctaca agaacgccta ccgccccgga agcaccaatc cgaccggcca
     4981 ggcgcccgag tcctactcgc tgccgaagat gctcgaagcg ctccgcccga agtacgaact
     5041 ggcgaagaag cgcgccgcgg agggctccac aacccgcttc aagaagggcg tcggcctttc
     5101 ggtcggcgtg tacggctgcg gcctcgacgg gcctgacggc tccgaagcgc ggctcgatat
     5161 gaacccggac gggacgatca cggtctgcac cgcgtgggaa gaccacggac agggcgccga
     5221 cgccggcgcg atcggcacgg cgcacgaggc gctgcgtccc ctgggaatct cccccgacaa
     5281 gctgaaattc acctggccga atacggcgaa gtgccccaat tccggccccg cgggcggctc
     5341 ccgttcccag gtcatgacgg ggaacgccat tcgcgtcgcc tgcgagacgc tactgcggga
     5401 gaccgcgaaa ccgaagacag gcttcctgaa acgggacggc ggctttatga cctacaacga
     5461 actggtcgcc gccggaaagc cgacctcctt caccggcaag tggtccgccg tcgagggaac
     5521 ggcctgcaac gaggacggac agggcaagcc gttcgtgatc tacatgtacg gcgtcttcat
     5581 ggcggagatc accgtggaca cggaaaccgg aaagacctcg gtcgacagga tgacgctgat
     5641 gtgcgactgc ggaaagatca acaaccgcct cgtcgtcgac ggtcagaact ggggcggcat
     5701 ggcccagggc atcggcctcg cgctgaccga ggacttcgaa gatatcgaga aacactccac
     5761 catgccgggg gcgggattcc cgtacatcaa ggacatcccc gacgatatgg agatcatcta
     5821 cttcgaggaa catcgtgaag acgggccgca cggcgcggca ggcatcggcg agcttccgct
     5881 ctccagcccg cacgcatcca tcatcaacgg catctacaac gcgacgggcg tccgtatcac
     5941 caggctgccc gcatacccgg agaaggtact ggccgggctg aaaaagtaac agtatccggg
     6001 gaggcgttcc tcgcggggcg cctccctccg ttccgggagg tacgccatgg aaaaagcacg
     6061 gctgctggaa ttcgaatcga agtgcgttca ggaggagcct ccgtactgcc aggcggcttg
     6121 tccgctgcat ctcgacggac gggccctctg tgcttttgtc cgcgaaggcc ggatcgacga
     6181 cgcccggaag ctgatcgaga ggacgctccc cctgcccgaa gcgcttgcgc gtatctgcga
     6241 cgcgccgtgc aaggaagcct gcctccgccg cgaactcggc ggcgcgatcg agttgggcgc
     6301 tcttgaaaga gcctgcgtcg aacaggggcc tctccggcgc gctccgctcc ttattccgaa
     6361 gaacggaaag agggtcgccg tcgtcggcgg cggactgagc gcgctctgcg cagcctcgga
     6421 aggcattcgc aaagggtact cggtcgccgt cttctgcgcg gaggaccagg ccgcggcgtt
     6481 gactgccctc tcccccctga tcaccgatga tatcgcgcgc cgcgagcttg cctggctcga
     6541 aaaaggcgga gtcctgtttc tgccccttga ggacctccct ccgctttcag agctttccgt
     6601 acgtttcgac gccgcctacg ccggtctgga cgacccgttc gcggcctgcg cgactgcgga
     6661 agcgactgct tcagtgaacc ccgtgagcct tgagacttcg gtcagaggac ttttcgccgg
     6721 aggcgccgcc ccctccttca tccttcgagc cgccgacgga cggcggggaa tgaagtcggc
     6781 ggagcgtttt ctccagggcg cgtccctctt ctcctcacgg gagaaggagg ggccgtacgc
     6841 cacccgtctg ttcacctcca ccgaggggat ggcttcagcg gagccggaag cggttccgca
     6901 ggaaggctac tcccccgaag aggccatccg cgaggcgaaa cgctgcatcc tctgcgagtg
     6961 catggagtgc gtgaaacact gcgcctacat gcagcactac aaagggtatc ccaagaagtt
     7021 cgcgcgggag atctacaaca acctctccgt gattcaggga acgaggctgg cgaacggcat
     7081 gatcaactcg tgcaccctct gcggccgctg cgaacgcatc tgccccaacg gcttctccat
     7141 ggccgatctc tgccgcgaag caaggcggga gatgctccgc caggagaaga tgcccccttc
     7201 ggcgcacgac ttcgcgctgg aggacatgcg cttcaacaac tctccaaggg cgacgctgtt
     7261 gcggcacgag cctggaaagg agacgagcgc ctacctcttc ctcccgggat gccggctcgc
     7321 gggctgcact cccgaacaga caaccgccct ctacgccttt ttgagggacc gtctggaggg
     7381 cggcgtcggt ttctggctcc gctgctgcgg cgctccggcc gactgggcag ggcgcgatga
     7441 agaactccac agggaagccg cgaagatcac ggaagagtgg gagtccatgg gttccccggt
     7501 cgtcgtggcc gcctgcacga gctgcctcca gaccttccgc aaggcaacgc cgtcaatcaa
     7561 ggccgtctca ctgtgggaga tcctggaggg cacggagctt ccggaggggg cgctctcttt
     7621 cgaacgaact cttgccgtcg ccgacccctg taccgctgcg caggcgcccg aaatcaggga
     7681 cgccgtgcgg aacgtcgcct cccgagcggg agtgcgcttc gaagagcctt tactttcagg
     7741 agaattctcc ttctgctgcg gcttcggcgg actccaggag agcgccaacc cgaccctggc
     7801 cgctctttcc gccaaaaccc ggggggagga gagttctctt gactttctcg tatactgcgc
     7861 catgtgccgg aatctcttcg cacaggcggg gaaacccgcc gtccacctgc tggatatcct
     7921 ctttccgcag ggagacgaag acccggccgg gcgtccagcc atcggctact ccgaacagca
     7981 ggagaaccgg gtcggactgg tccgcgcgct gcgcgcatct ctctggaagg aggaagacat
     8041 cgtgaacgaa ccgcatgaat ccatccggct tgtcatcccg cccgacgtgg aaaagactct
     8101 ggcgaagcgg cgaattcttg ccgataccgt ccggagagcc atctggtcgg cggaacgaag
     8161 cggacgcaag atgaaacaga tcaacggcgc gctggtcgct tcgctgcgcc cggcgtcggt
     8221 cacctactgg gtggagtacg ctcccaaagg cgacggctct ttcgaggtgc gcaacgggtg
     8281 gagccacaga atgttcgtcg ccggagccgc ggagaatctt gtccccgacg cttccggagg
     8341 agacggagca tgaaagagct gatcaagtcg ttcgagaagt acacgaagac cgaatgcggc
     8401 gcatgcggcc gttttctgga gttgcaaaag accgagatca cctatctcgg cagcgtcttc
     8461 acgctggatt taccggtctg cccatcctgc ggcacggttt tcgtccccga agagctggcc
     8521 acggggaaga tgctcgaagt ggagaagatt ctggaggaca agtaatggat gttttcgatc
     8581 tcgacgacct gcagatggag atgctctccc tcgaagggaa ggggtaccac tgcagccaga
     8641 ttctcatact gctggcgctt cgccaaacag gaggagagcg caacgactcg ctgatccgtt
     8701 cgatgggagg gctctccctc ggactcggtc acagcaacgg cacgtgcgg
//
LOCUS       DAOPKU010000046         8678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8599_length_8678_cov_4.273687, whole genome shotgun sequence.
ACCESSION   DAOPKU010000046 DAOPKU010000000
VERSION     DAOPKU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8599_length_8678_cov_4.273687"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4575)
                     /locus_tag="PK849_02460"
     CDS             complement(<1..4575)
                     /locus_tag="PK849_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65016.1"
                     /translation="MQCANRIRTILRSLPTLLSVCCLLFFAALPYAGAQERKAPGPVQ
                     VEVKAQDGTLLSGVTVFLGGRYAPSGADGRAVFDGVPEGKQRLAVEHIDYDAFRGNVD
                     LAAGVRRPLGVKLNPVVWSKISGKVLLEGAEQPLPGVRVSIAPKSVKATLQGPVEFMT
                     GWDGAFVVSGLPPGVYKVELSHSGCAPKSFDLQVAPNMQEVSWSLAPLFDTASLTVTV
                     RDGDGGKEIASARVVLAEAWPRGEIAQKTTDASGSAAFAGLKIGRLNRAGADGRMTLS
                     RSAVTVRAEADGYEPMLVPVLLGRTASVEVQLNSAEKIAEQPGNLDFSSAQRIRTGAP
                     VEFVIENAGENKFFRFHLPYPAWVGLDANRGQNPWLRFHLHAADGTEIGNWGLSGESA
                     LFDAGALPAGEYVLQAREWNNNKAATLPMTFTIQCRWASDPHSPNAELSSARAFRTGE
                     EVRGYILPRGDRNHFRFEIKRPGQVRLWLQRPENIWIRAFLFRSDGAEIANLGCSAAT
                     AEAVYRLDPGAYVVDIREWNNNAASLDPYTLRIEEIEDDGMDDAKQEEASVRIFRPLP
                     IASLRGGTIFPQGDRDWYQATLPSAGVFKAFLLRSPSVWQRITLFDTKGVRLTDAGVS
                     GPRGDLAWHADGPATVFLQVREWNDNAADPSPYILGTFFEPCDQLDAQGRNDSPDTAS
                     PLELNEWTRGNLLPNGDNDWFRIDVDHPGILRLEGTTPPGIWVRLALHGGNAEQLADV
                     GYSRPSSEFTWENPVLPGAYFIRLREWNNNASNPGIYSLRAVLQRSEPEESVPLAEDA
                     VRTLTPGEARPFFIDHGGDRDVFLFDIAQQGPFWLKIWKPGNIWIRARLYDDRTGAEL
                     KKIDLSGASYNGKFEAEGPTRYRLELGEWNNNAYSVQPGFIQVRPDDAQITGDELKAV
                     PDAVTPTLVTFTRSAWAHMQIPRARTIEVDATGDGKTDFSFRPGDTATWRYPAEGLYK
                     AAALMQGEGGATTRLPFWVDVSGPRERTGIHLTVDVPGEGQRVEDEAPARARAMSYSG
                     SPIARIEASVDGTQLPPAYTPPYTFEVPWKSLPRAAKEHLFTFKAVDGRGESATVERR
                     ISISEYFGLRPADGAVLSGNQVRVSWSSGEFSPTKVRYRAKGTEEWTEIVGESGREHA
                     VFLEDLESGTAYEFQPLGGSEEGPVRTLTRVKGLAFGKSRYGASIERTYDQRFPISVR
                     NHAEEAMKVVLTSGKLEEEGLFIGFVGEGSEGEPFDLAPGEEREFLLVLNAQNAVKPK
                     HVFPVKISSANGFADEAEVEVEVRLPVVKLEWEDLGEAPKGPGRRLRLVNRGDSLTDL
                     SVFTDTGELKVSPAINHGFLPSGQSVELTVTPRLYEGFREARGAVTAKSIATESSFPV
                     HLALPEGKEIFGVNLMPKMPEETADEDERVLLEARALSAAFLTPDYINASMWGHGIDS
                     NGDGRIDRWSYIDQEERILWVGEDASGDGEIDFVRADVGFDGRFDYSALKGAKGWEET
                     NLLDAWLEMTFTLPWNRGSYEPHDLDIVMNG"
     gene            complement(4698..6275)
                     /locus_tag="PK849_02465"
     CDS             complement(4698..6275)
                     /locus_tag="PK849_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65017.1"
                     /translation="MRFFSVRRFCVFMIAWAVAGGFSGAASALENTWTYQRIDSLYIE
                     IPDSWSPYSKKGSSGFYTGKPPSGGASVGSLVLLAGREKRNRYEDVLAPLRRSPGGSI
                     EALFHEEEKVVGGKDAVLFTNEISRREKDEAARPFIRMRSVVWKEAAEDGVLLLAVGG
                     DAALFDEHAADIDRMIASVRFDEPPLRQPGRKLEGIGGHAAVGEPVLPPIDLPPIELE
                     SGETAENEETKEGPPAEKTSRERPVFNSDALLEEEIRKARTALSLRDEGELLLAKGDR
                     EGAMDRFRGSLANVPDEDLSAFVETLGRRASEEHKAEEGRASLLRREGMELQREGKLH
                     EALTKYRESLAVNDDETVREIVLGLEKLLQERASASVAQGEAHEAGGRLEEARAAYAE
                     SLSRVDEEFVRRRIAAVERLIRERQEAASPERVLAATLRNEGKALEEEGRLYEALGKY
                     RESLKSYEEQELLTRADALETELKERARARIREGGALQRAGKHAEALEKFRESRRYYP
                     RSEVDEHIRKLEEFLKK"
     gene            complement(6310..6852)
                     /locus_tag="PK849_02470"
     CDS             complement(6310..6852)
                     /locus_tag="PK849_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65018.1"
                     /translation="MTEMRCLRFLPILLILLFLQGAAGPLLASPETAPAASEGAQRLS
                     RLETAALLRGRGVAHEDAGRLENAVRNYEESLLFFPDAEVSQRIDLLKKTLAERTAPL
                     SPPAPLLPEARVSRLEKARALREDGKNAFVRGELHDAARFFEESLSLFEDPELSRFAG
                     AVKKEIELSSSLEGLEELFD"
     gene            7091..7876
                     /gene="nudC"
                     /locus_tag="PK849_02475"
     CDS             7091..7876
                     /gene="nudC"
                     /locus_tag="PK849_02475"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HPE65019.1"
                     /translation="MDLCSTQGAWLLFHNDMVLVKSKEPLTLPSEDEGAAFTDKLLHR
                     GALSPARGAYSWGEYPADEPAPQGFEPVGLRELWALGGDSIFHSAGTAFQMMDWRRNC
                     RYCPRCATLMQPAEEGRAYACPQCAYVSYPTLSPAIIVAVVRDGKLLLAHGTRFPSGR
                     YSVLAGFVEPGESLEETVEREIFEEVGIKVKDIAYFGSQPWPFPHSLMLGFTARWASG
                     EIVPDGVEIGDADWFTPENMPEIPPSISISRKLIDHWLSGGIA"
     gene            7902..>8678
                     /locus_tag="PK849_02480"
     CDS             7902..>8678
                     /locus_tag="PK849_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65020.1"
                     /translation="MIRALGILVFLAFALALHPNAEAEAKEIEKGLSRGSVLLSWFKD
                     AEMDFQLLRSLGADVAGGGAAGEIFVTAKGITDGNPASWSAAFLALGERLEADGNARL
                     ERGHKVSAMESLLRAASYYRAAEYYGDPSAPETAQTGMKCRDVFMKGMQLAAWKTEGV
                     LIPFGKENDFLPGYFLTPAKGSGKRKTLIAQSGFDGTAEEMYFAVGKAALERGYNVLL
                     FEGPGQTGKRRFHPDSSFVPDCAPALRAVVDFALSRPEVHP"
BASE COUNT         1494 a         2885 c         2688 g         1611 t
ORIGIN      
        1 accgttcatc acgatgtcga ggtcgtgcgg ctcgtagctc ccgcgattcc atggaagggt
       61 gaaggtcatt tcgagccaag cgtcaagcag gttggtctcc tcccacccct tcgcgccctt
      121 gagcgccgag tagtcgaagc ggccgtcgaa gccgacgtcg gcgcggacga agtcgatctc
      181 tccgtccccg ctcgcgtcct ctccgaccca gaggatgcgc tcctcctggt cgatgtagct
      241 ccatcggtcg atcctcccgt cgccgttgga atcgatcccg tgcccccaca tggaggcgtt
      301 gatatagtcg ggcgtcagga aggccgcgga gagcgccctg gcctcaagga gcacacgctc
      361 atcctcatcc gccgtctcct ccggcatctt cggcatcagg ttgacgccga agatctcctt
      421 cccttcggga agcgccaggt ggacagggaa cgacgactcc gtggcgatgg acttcgccgt
      481 caccgcgccc cgcgcctcgc ggaacccctc gtagagcctc ggcgtcacag tgagctccac
      541 agactgcccc gaagggagaa atccgtggtt gatcgccgga gagaccttca gctcgcctgt
      601 gtccgtgaag accgaaagat cggtaaggct gtcccccctg ttcaccaggc gcagacggcg
      661 tcccggcccc ttcggcgctt cgccgagatc ctcccactcc agcttcacga cggggaggcg
      721 cacctccacc tccacctccg cctcgtcggc gaaaccgttc gcggagctga tcttgacggg
      781 gaagacgtgc tttggcttca cggcattctg cgcgttgagg acgagcagga actcccgctc
      841 ctcgcccggc gccagatcga acggctcccc ctccgacccc tcgccgacga agccgatgaa
      901 gagcccctcc tcctctagct tgccggaggt gagcacaact ttcatcgcct cctcggcgtg
      961 gtttctgacc gaaatgggga accgctggtc gtaggtgcgc tcgatggacg cgccgtagcg
     1021 gcttttgccg aaggcgagcc ccttgacccg cgtgagggtc cgcaccggcc cctcctcgct
     1081 ccctccgagc ggctggaact cgtacgccgt tccggattcc agatcctcaa ggaatacggc
     1141 gtgttcgcgg ccgctctcgc ccacgatctc cgtccactct tccgtcccct tcgccctgta
     1201 gcggaccttc gtcggcgaga actccccgct cgaccagctc acgcggacct ggttgccgga
     1261 aagcacggcc ccgtccgccg gacgcaggcc gaagtactcg gaaattgaga tccggcgctc
     1321 gacggttgcg gattcgcccc gcccgtcgac ggctttgaac gtgaagagat gctccttcgc
     1381 cgcgcgggga aggctcttcc acggcacctc gaacgtatag gggggcgtgt aggcgggcgg
     1441 gagctgcgtt ccgtcgaccg aggcctcgat gcgcgcaatg ggcgagccgc tgtagctcat
     1501 ggccctggcg cgcgccggag cctcatcctc cacgcgctgc ccctctcccg gaacgtcgac
     1561 cgtcaggtga atccccgtcc gctcccgtgg gccggagacg tccacccaga agggaagccg
     1621 cgtcgtcgcg ccgccctcgc cttgcatcag cgcggcggcc ttgtacagcc cctccgcggg
     1681 gtagcgccac gtggccgtgt cgcccggacg gaatgaaaag tcggttttcc cgtcgcccgt
     1741 cgcgtcgact tctattgtgc gtgcgcgggg gatctgcatg tgcgcccacg cgctccgggt
     1801 gaaggtcacg agcgtcggcg tcacggcgtc gggaaccgcc ttgagctcgt cgcctgttat
     1861 ctgcgcgtcg tccggacgca cctgaatgaa gccgggctgg acggagtacg cgttgttgtt
     1921 ccactccccc agttcaagcc ggtagcgcgt cggtccctcc gcttcgaact ttccgttgta
     1981 ggaagcgccg gagagatcta tcttctttag ctccgctccc gtcctgtcgt cgtagaggcg
     2041 cgcgcggatc cagatgttcc caggcttcca tatcttcagc cagaacggcc cctgctgtgc
     2101 gatgtcgaag agaaagacgt ccctgtcgcc tccgtgatcg atgaaaaacg ggcgcgcttc
     2161 tcccggcgtg agcgtgcgga ccgcgtcctc ggccagcggt acgctctctt ccggctcgga
     2221 ccgctggagc accgcacgga gagagtagat gccggggttg gaggcgttgt tgttccactc
     2281 ccggaggcga atgaaataag ctccggggag aacggggttc tcccatgtga attcagagga
     2341 ggggcgtgaa tatcctacgt cggcgagctg ctcggcgttc ccgccgtgga gagcgagccg
     2401 cacccagata cccggaggcg tcgtcccctc cagacgcagg attcccggat ggtccacgtc
     2461 gatgcggaac cagtcgttgt cgccgttcgg gagcaggttg ccgcgcgtcc actcgttcag
     2521 ttcgagcgga gaggccgtat ccggcgagtc gttccgcccc tgggcgtcga gctgatcgca
     2581 cggctcgaag aacgtcccca ggatgtaggg cgacgggtcg gcggcgttgt cgttccactc
     2641 ccttacctgg aggaagaccg tcgcaggtcc gtcagcgtgc caggcaagat caccgcgagg
     2701 tccgctcact ccggcgtcgg tcagccgcac ccctttcgtg tcgaagagcg tgatgcgctg
     2761 ccatacagaa ggggaacgga gcaggaacgc cttaaagacg cccgcgctcg ggagcgtcgc
     2821 ctgataccag tccctgtccc cctgggggaa gatcgtcccg ccgcgcagcg aggcgatcgg
     2881 aagcggcctg aagatacgga cagaggcctc ctcctgcttc gcgtcgtcca tgccgtcgtc
     2941 ttcgatctcc tcgatgcgga gcgtgtaggg atcaaggctg gcggcgttgt tgttccattc
     3001 gcgaatgtcg acgacgtacg cgccggggtc gagcctgtag acggcctccg ccgtcgccgc
     3061 cgaacagccg aggttcgcta tctcggcgcc gtcggaccgg aacagaaagg cgcgtatcca
     3121 gatattctcc ggccgttgga gccagaggcg tacctggccg ggccgcttga tctcgaaccg
     3181 gaagtgattc ctgtccccgc gcgggaggat ataccctcga acctcctcgc ccgtccggaa
     3241 agcccgtgcc gaggagagct ccgcgttcgg agaatgcggg tcggaggccc agcggcactg
     3301 gatcgtaaag gtcatgggca acgtcgccgc cttgttgttg ttccactccc gggcctggag
     3361 cacgtactcg cctgccggaa gcgccccggc gtcgaagagc gcgctctctc cggagagccc
     3421 ccagttgccg atctccgttc cgtccgccgc gtgcagatgg aagcgtaacc acggattctg
     3481 tcctctgttc gcgtccagtc cgacccatgc ggggtacggc agatggaagc ggaagaactt
     3541 gttctctccc gcgttttcga tgacaaactc tactggagcg ccggtcctga tccgctgcgc
     3601 cgaggaaaaa tcgagattgc cgggctgttc agcgatcttc tccgccgagt tgagctgcac
     3661 ttccacggac gccgtccgac cgagaaggac ggggacgagc atcggctcgt acccgtcggc
     3721 ttcggcgcgc actgtgacgg cgctcctgct gagcgtcatc ctgccgtcgg cgcctgcgcg
     3781 gttgagccgc ccgatcttga ggccggcaaa ggccgccgat ccgctcgcat ccgtcgtctt
     3841 ttgggcgatc tccccccggg gccacgcctc ggcgagaacg acgcgcgcgc tcgcgatctc
     3901 cttgccgccg tcgccgtccc tgacggtcac ggtgagcgag gccgtatcga agagcggcgc
     3961 aagcgaccac gagacctcct gcatattcgg cgcgacctgg aggtcgaagg acttcggcgc
     4021 gcatccggaa tggctcaact cgactttgta gacgccgggc ggaagcccgg acacgacgaa
     4081 ggcgccgtcc cagccggtca tgaactccac aggcccctgg agcgttgctt tcacgctctt
     4141 cggagcgatg gagacgcgca cgcccggcaa gggctgctcc gcaccttcaa gaagcacctt
     4201 ccccgagatc ttgctccaga cgaccggatt gagcttgacg ccgagaggac gccgcactcc
     4261 ggccgccaga tcgacatttc cccggaaggc gtcgtagtcg atgtgttcaa cggcgagacg
     4321 ctgttttccc tcggggacgc cgtcgaagac ggcccttccg tccgcgccgg acggagcgta
     4381 cctgcccccg agaaagaccg tcactccgga gaggagcgtt ccgtcctgcg ccttgacctc
     4441 gacctgcaca gggccgggag cttttctctc ctgcgctccg gcgtacggga gcgcggcgaa
     4501 aaagagaaga cagcacaccg acagaagcgt cggaaggctg cggaggatag tgcggattct
     4561 gttcgcacac tgcatcgcgg ctcactctcc ttttacagca tctgcgtgca gtaccccggc
     4621 acgctctttc cgaacccggc ttttcgctcc gggacgactt gaggccggag aggcgacgtc
     4681 cgcttctccg gtacgcgcta ttttttcaag aactcctcca gcttgcgaat atgctcgtca
     4741 acctcgctgc gcgggtagta cctccggctt tcacggaact tttcgagggc ctccgcatgc
     4801 ttcccggcac gttgcagcgc tccgccctcg cggatacgcg cgcgggcccg ctctttcaac
     4861 tccgtctcca acgcgtcggc gcgcgttagc agctcctgtt cctcgtatga tttcaggctt
     4921 tcgcggtact tcccgagcgc ctcgtagagg cgtccctctt cttccagcgc cttgccttca
     4981 ttccgaagcg ttgcggcgag gactctctcc ggggacgcgg cctcttgccg ctcccggatc
     5041 aggcgctcca ccgcggcgat tctgcgccgg acgaactcct cgtcgacccg ggaaagactc
     5101 tccgcgtacg cggcgcgcgc ctcctcaagc cgccctccgg cctcgtgcgc ctccccctgc
     5161 gcgacagagg ccgaagccct ctcctgcagg agtttttcga gtccgaggac gatctccctc
     5221 accgtttcgt cgtcgttcac ggcgaggctc tcacggtact tcgtcagcgc ctcgtgcagt
     5281 tttccctccc gctgcaactc catcccctcg cggcgcaaga gagaagcgcg gccttcctcg
     5341 gccttgtgct cttccgacgc ccgacgcccc aaagtctcca cgaaggcgga cagatcctcg
     5401 tccggcacgt tcgcgagact tccgcggaaa cggtccatcg cgccctcccg gtcgcccttc
     5461 gccagcagga gctctccttc gtccctgagc gacagcgccg ttcgcgcctt ccgaatctcc
     5521 tcttcaagaa gcgcgtccga attgaagacc gggcgctctc gcgacgtctt ctccgccggc
     5581 ggaccttcct tcgtttcttc gttctccgct gtttctccgc tctccagttc gataggcggc
     5641 aggtcgatgg gcggcagaac cggctccccg acggcggcgt gtcctccgat tccctcaagc
     5701 ttcctgccgg gctgccgcag gggcggctcg tcgaagcgca cggaagcgat catccgatca
     5761 atgtccgcag catgctcgtc gaagagggca gcgtctcccc cgaccgcgag gagcagcacc
     5821 ccgtcctccg ccgcttcctt ccagacaacg ctccgcatac gaatgaaggg gcgcgccgct
     5881 tcatcctttt cccggcggct gatctcgttc gtgaagagga cggcgtcctt ccctccgaca
     5941 actttttctt cctcgtggaa gagcgcttcg atgctccccc cgggagatcg cctcagcgga
     6001 gcgaggacgt cttcgtagcg gttccttttc tccctcccgg cgagcagcac cagggaacct
     6061 acggaagcgc ctccgcttgg aggtttgccg gtataaaaac cggaagagcc tttttttgaa
     6121 tagggggacc atgaatccgg aatttcgatg tacagcgagt cgatacgctg gtacgtccag
     6181 gtattttcaa gagcggacgc cgccccggag aaacctcccg cgacagccca ggcgatcatg
     6241 aagacacaga aacggcgaac agaaaagaat cgcatgccac acacctccat agcgaataaa
     6301 atctcccggt caatcgaaca gctcctccaa cccctcaaga gaggacgaga gctcaatctc
     6361 cttcttcacg gccccggcga agcgggagag ctccgggtcc tcgaagagcg agaggctttc
     6421 ttcgaagaaa cgcgccgcat cgtgaagctc gcccctgaca aacgcattct tgccgtcctc
     6481 ccgcagagcg cgcgcttttt caagacgaga aacccgcgcc tcgggaagca ggggcgcggg
     6541 cggggagagc ggagcggtac gctcagcgag cgttttcttc aaaagatcga tccgttgcga
     6601 aacctcggcg tcggggaaga agagcaggct ttcttcatag ttccggaccg cgttctccag
     6661 cctgccggcg tcttcatgcg caacgcctcg cccccggaga agagcggccg tttcaagacg
     6721 cgaaagacgc tgcgcgccct ccgatgctgc cggagccgtt tccggcgagg cgagaagagg
     6781 acctgccgcc ccttgcagga agaggagaat cagaagaatg ggcaggaagc gcaggcatct
     6841 catttccgtc ataacatctc ttcctttcca taaaaaagtg gtgtatcaac tgccgaatct
     6901 tatgttttct gaattatacc cctcttcgca ggggagtaca atgggcgtgc gcttcaaaac
     6961 agggagcgcc tgttccgcac tccgcgcctt ctccgtgggg aaaaaacatt ggattcgaaa
     7021 ggcgcgtatg atagaatttt cgggcggact tccttcatat aaggcggccg caaaggagga
     7081 taacgatcac atggatttat gcagtacaca gggcgcctgg ctgctctttc acaacgacat
     7141 ggttctggta aagtcaaaag aacccttgac gcttccatcc gaggacgagg gcgcggcgtt
     7201 tacggacaag ctgctccacc ggggagctct ctcccctgcc cggggcgctt attcatgggg
     7261 ggagtatccc gccgacgaac cggcgccgca gggcttcgag ccggtcggcc tgcgcgagct
     7321 gtgggccctc ggcggagatt cgatctttca ctcggcgggc acggcgtttc agatgatgga
     7381 ctggcggcgg aactgccgct actgcccccg ctgcgccact ctcatgcagc cggccgagga
     7441 ggggcgggcc tacgcctgtc cgcagtgcgc gtacgtctcc tacccgacgc tcagcccggc
     7501 gatcatcgtg gcggtcgtgc gcgacggcaa acttcttctg gcgcacggaa cccgctttcc
     7561 ttcggggcgc tacagcgtgc tcgcggggtt cgtcgagccg ggcgagagcc tggaagagac
     7621 tgtcgaacgg gagatcttcg aggaagtcgg catcaaggtg aaggacatcg cctacttcgg
     7681 cagtcagccg tggccgtttc cccattcgct gatgctcggc ttcaccgcgc gctgggcaag
     7741 cggggagatc gttccggacg gagtcgagat aggggacgcc gactggttca cacccgagaa
     7801 tatgccggag atccctccat ccatcagcat ttcgcgaaaa ctgatcgacc actggctctc
     7861 cggaggtatt gcgtaacaca gtcagggagg tggaacaacg gatgatacga gcgctcggaa
     7921 tactggtatt ccttgccttc gccctcgcgt tgcatccgaa cgcggaagcg gaggcgaagg
     7981 agatcgagaa gggactgtcg cgcggcagcg tcctgctctc atggttcaag gacgcggaga
     8041 tggactttca gcttctccgc agccttggcg cggacgttgc ggggggcgga gcggcagggg
     8101 aaatcttcgt gaccgcgaag gggataacgg acggaaatcc ggcctcgtgg tcggcggcct
     8161 ttctcgcgct gggagagcgg ctggaagcgg acggaaacgc ccggctggag cgggggcaca
     8221 aggtcagcgc gatggagagc ctgctgcgcg ccgcgtcgta ctacagggcg gcggagtact
     8281 atggagatcc gtcggctccc gaaaccgcgc agacgggaat gaagtgccgc gacgtcttca
     8341 tgaaggggat gcagcttgcg gcgtggaaga cggagggcgt gctcattccg ttcggcaagg
     8401 aaaacgattt tctgcccggc tacttcctca ctccggcgaa gggaagcggc aagcggaaga
     8461 cgttgatcgc gcagagcggc ttcgacggca cggcggagga gatgtacttc gccgtcggaa
     8521 aggcggcgct tgaacggggg tacaacgtgc tcctcttcga gggaccggga cagacgggga
     8581 agcggcgttt ccaccccgac tcgtccttcg tccccgattg cgcccccgca ttgcgggccg
     8641 tcgtggactt cgcgctctcc cgccccgaag tacatccg
//
LOCUS       DAOPKU010000047         8640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8637_length_8640_cov_5.386954, whole genome shotgun sequence.
ACCESSION   DAOPKU010000047 DAOPKU010000000
VERSION     DAOPKU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8637_length_8640_cov_5.386954"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1629)
                     /locus_tag="PK849_02485"
     CDS             complement(148..1629)
                     /locus_tag="PK849_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPE65021.1"
                     /translation="MTDKTYDIIIVGAGIVGATLARELSAYALEIAVIDKAADIPSGA
                     SRANSAMIHAGYDDKPGSIKADFCAPGNRLYQELAGPLDITLRKCGSYVCGFDAKDLK
                     HLEKLLDQGKRNGVPGVEIITGDALRAKEPHVSAEIAHALWAPSGCIVNNFEAVLAFM
                     DNAQQNGVELFLETELQDIVLSDDRKEVLGIRTNRGFMRTSVVINAAGINSDLVAKMA
                     GDDSFSIEPTRGEYYIMDKNVGNLVGSFFFPCPTDKGKGITVAPTAENNLLIGPTSVV
                     QESRDDTSTSVDGLKEVVDGARLLVPAIPLGMAITTFSGLRASASTKDFVLGVLDTPR
                     GFVNVAGIKSPGFTSAPALAKHTVELMKEKLGDRLSFASKPDFVPERRHIPRFEALPM
                     EEKIRLAAKDPRYAQIVCRCEMVTEAQIVEAVARGARTVAAVKIWTRAGAGRCQGGFC
                     GPRVVDILARELGVSPEEITRHGGHSRYLVGRTKDYWFKGGEK"
     gene            complement(1777..3288)
                     /gene="glpK"
                     /locus_tag="PK849_02490"
     CDS             complement(1777..3288)
                     /gene="glpK"
                     /locus_tag="PK849_02490"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPE65022.1"
                     /translation="MSEKKYVVAIDQGTTSSRAIVFDLEGTPKNSHQMEHEQIYPKPG
                     WVEHNPMEIWSRVQDVIREVLNTNGVKADEIGAIGITNQRETTVVWEKATGKPVYNAI
                     VWQCMRTQDFCAEWQKKPGWDKKVNDLTGLVISPYFSGTKIKWILDNVPGVRAKAEKG
                     EVLFGNIDTWVTWNLTGGPNGGVHVTDVTNASRTLLMNIETLKWDKEMMDFLGVPEAM
                     LPAIKPSSYVYGYTPESGPFGAKIPVSGILGDQQAALFGQTCYSPGEAKNTYGTGCFM
                     LLNIGDKPVRSKNGLLTTVAYGLEEGKATYALEGSIAITGAAVQWLRDNLRLFDDAPD
                     SEYFAAKVKDSGGIYFVPAFSGLYAPYWDMSARGAILGLTRYITKEHIIRATLESICY
                     QTRDVQAAMDADSGVPLKALKVDGGAVKNNLLMQMQADILGVPVVRPVVNETTALGAA
                     YAAGLATGLWKSTEELRKHWKVDKQFDAAMPADKREEMYKGWKKAIEKSKGWL"
     gene            complement(3451..3849)
                     /gene="dhaM"
                     /locus_tag="PK849_02495"
     CDS             complement(3451..3849)
                     /gene="dhaM"
                     /locus_tag="PK849_02495"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HPE65023.1"
                     /translation="MNAILVVSHSAEAARGIVAIASQMAGERVGIAACGGTDDGELGT
                     SVPSILSALEGLLETSDGVLVIPDLGSAVLAARTAKDLLGERAGRVLIADGPVLEGTL
                     MASVEASVGAGLDRLAAVVDETRGLRKLQE"
     gene            complement(3850..4488)
                     /gene="dhaL"
                     /locus_tag="PK849_02500"
     CDS             complement(3850..4488)
                     /gene="dhaL"
                     /locus_tag="PK849_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HPE65024.1"
                     /translation="MNLDVQGMLKVFDAIGETMAAHKEYLTELDSAIGDADHGINMNR
                     GFHKVHDKLSAGSYTDLGALFKDVGMTLMGNVGGASGPLYGTFFMRMSTKLAGRQDVD
                     TVSLADAMEEGLKGIVALGKAQLQDKTMVDALTPAVEALKKASDNGVDLCGALEAGVA
                     AAQSGMESTIPLVARKGRASYLGERSAGHQDPGATSIYLILSTILRSVKESS"
     gene            complement(4499..5497)
                     /gene="dhaK"
                     /locus_tag="PK849_02505"
     CDS             complement(4499..5497)
                     /gene="dhaK"
                     /locus_tag="PK849_02505"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HPE65025.1"
                     /translation="MKKLLNKVDDVVVESLAGLAAAHPGLVKLHPSVKAVLRADAPQK
                     KVAILSGGGSGHEPLHGGYVGYGMLDAACPGEVFTSPTPDQMYEAAKAVDGGKGVLFI
                     VKNYTGDVMNFQMAADLLAADGIPVAQVVVDDDVAVQDSLYTAGRRGVGGTVLIEKIV
                     GAKAEAGASLEEVKALCEKVNSQVRSMGMALTSCAVPAAGKPTFELGEDEMEIGIGIH
                     GEPGRERMKLKTAKEIVEMMAKAIVDDLPFKAGDEVVAFVNGMGGTPQMELYLAYNDL
                     VNFLKGKGIVIARSLVGNYITSLEMQGFSITLLKLDGEMKALWDAPVETAGLRWGR"
     gene            complement(5603..6340)
                     /locus_tag="PK849_02510"
     CDS             complement(5603..6340)
                     /locus_tag="PK849_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HPE65026.1"
                     /translation="MTNLLGEFFGTMTLVVFGDGLVACALLNKSKGQNAGWVHITIGW
                     GAAVVMGVFAATSLGAPQADINPAVTLAKMLAGTYTLPHAMMTMLAQVGGGILAGVVV
                     WLAYLGHWEATEDKGLKLAVFSTGPAIRNPINNFITEMIGTIFLVVPIMFIFSSGVKE
                     ALGGLTPGFGPFLVGWLVMVIGMALGGPTGYAINPARDLGPRIAHFVLPIAGKGDSDW
                     GYAWIPVLAPLTGGAVSYFIAHALGVM"
     gene            7084..7449
                     /locus_tag="PK849_02515"
     CDS             7084..7449
                     /locus_tag="PK849_02515"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPE65027.1"
                     /translation="MESILSISKALGEKNRLRVFLALIHTKELCVCEVGELLGLAPAT
                     VSRHMSILSRAKLVDSRKRGKWVYYCIKPAIAPELLQWIKNSVVDSFSVAEDVKRIEI
                     LVRERQDKDCTKACVSEER"
     gene            7456..7911
                     /locus_tag="PK849_02520"
     CDS             7456..7911
                     /locus_tag="PK849_02520"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPE65028.1"
                     /translation="MSKKPKVLFLCTGNSCRSQMAEGWTRHFHSAAIEAHSAGIETHG
                     LNPFAVEAMKEAGADISGQRSKNVMELLDVSFDYVVTVCGDADERRPFFPGETRVLHV
                     GFDDPPKLALLEETEEGKMNCYRRVRDEIRAFIKDLPGFLENLPEEKRS"
     gene            7919..>8640
                     /gene="arsM"
                     /locus_tag="PK849_02525"
     CDS             7919..>8640
                     /gene="arsM"
                     /locus_tag="PK849_02525"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HPE65029.1"
                     /translation="MDDIRNYVKERYAQAIKNKSSCCGSGGCCCGTTGASGIPSMTRG
                     NYDGATLEKTPEQMADQSFGCGNPFTEARIHPGETVLDLGSGAGLDLFLASEMTGPYG
                     KVIGLDMTDEMLATAAENLKGIENVSLVKGYIEEMPVESNSVDVIISNCVINLSPDKG
                     KVLKEAFRVLRPGGRFCVSDTVFLRPVTERAVKNLAAWSGCISGALRETEYAEKMREA
                     GFEEVEVRRTKVYSFPDAMAAMA"
BASE COUNT         1732 a         2549 c         2591 g         1768 t
ORIGIN      
        1 ggtggatgca ctgctgcagg atacccccca ggtcccagtc acgttcgagg acgagcacgt
       61 tctccgctcc ggcctctctg gcgccgatcc ccgctgcaac gccggcaggg cctgccccga
      121 tgatcgcaac atcaacatgc atgtcgctca tttttctccc cccttgaacc agtagtcctt
      181 cgtcctgccg acgagatagc gcgaatgccc gccgtggcgg gtgatctcct cgggggagac
      241 gccgagttcg cgcgcaagaa tgtcgaccac gcgggggccg cagaaacctc cctgacagcg
      301 tccggcgccg gctctcgtcc atattttgac ggcggcaaca gtccgcgctc cccttgcgac
      361 ggcctcgacg atctgcgcct cggtcaccat ctcgcagcgg cagacaatct gcgcgtagcg
      421 ggggtccttc gccgcaaggc ggatcttctc ctccatcgga agcgcctcga agcgggggat
      481 atgtcttctc tccggaacga aatcgggttt cgaagcaaat gaaaggcggt cgccgagctt
      541 ctctttcatc agttccacag tgtgcttcgc caaggcgggc gcgctggtga agccgggcga
      601 cttgattcct gcgacgttga cgaagccccg cggggtgtcg agcacgccga ggacaaagtc
      661 cttggtgctc gcactcgccc ggagccccga gaaagttgta atggccatgc cgagcgggat
      721 ggcgggaacg agaaggcggg ccccgtcgac gacctccttc agaccgtcga ccgatgtgga
      781 cgtgtcgtca cggctctcct ggaccacgga ggtggggccg ataaggaggt tgttctccgc
      841 cgtcggcgca actgtaattc ccttcccctt atcggtcggg cacgggaaga aaaagctgcc
      901 gacgaggttg cctacgttct tgtccatgat gtagtactcg cctctcgtcg gttcgataga
      961 gaatgaatcg tcgccggcca tcttcgcgac aaggtcggag ttgatcccgg ccgcgttgat
     1021 gacgacggag gtacgcataa agccgcggtt ggtgcgtatt ccaagaacct ctttccggtc
     1081 gtccgagagg acgatgtcct gaagctctgt ttcgaggaag agctcgacgc cgttctgctg
     1141 cgcattgtcc ataaacgcga ggaccgcttc aaagttgttc acgatgcatc cggagggcgc
     1201 ccacagcgcg tgggcgatct ccgcgctgac atgcggctcc ttggcgcgca gggcatcgcc
     1261 ggtgatgatc tccactccgg ggacgccgtt gcgctttccc tggtcgagaa gtttttcaag
     1321 gtgcttcaaa tctttggcat cgaagccgca gacgtaggaa ccacacttcc ggagcgttat
     1381 atccagcgga ccggccagtt cctgatagag tctgttgccg ggggcgcaaa aatccgcctt
     1441 gatggagccc ggcttgtcgt cgtatccggc gtgaatcatg gcgctgttgg ccctgctcgc
     1501 cccggaggga atatccgccg ccttgtcgat gacagcgatt tcaagggcat aggcggaaag
     1561 ctccctggcc agggtagccc cgacgatccc tgcaccgacg atgatgatat cgtaggtttt
     1621 gtccgtcaac ttcagaacac ctccatagta cgaagaggga gaaaggtgcg tgccatgggc
     1681 agcgtacggc ctctctccct ctgtcgcgca gtgcgattta tttcagaaga cttctcaaaa
     1741 tcggccgctg cgagtgtccg cagacggcgt cagaatctag agccagcctt tggacttctc
     1801 tatggccttc ttccacccct tgtacatctc ttcgcgcttg tctgcgggca ttgcggcgtc
     1861 gaactgctta tcgaccttcc agtgcttgcg cagctcctcg gtgctcttcc agagaccggt
     1921 ggcaagtcct gcggcgtatg ctgcgccgag cgccgtggtc tcgttgacga ccgggcggac
     1981 gacgggcacg ccgaggatgt cggcctgcat ctgcatgagg aggttgttct tgactgcgcc
     2041 gccgtcgacc ttcagggcct tcagaggtac gccggagtcg gcgtccatag ccgcctggac
     2101 gtcgcgggtc tggtagcaga tgctctccag cgtcgcgcgg ataatgtgct ccttggtgat
     2161 gtagcgggta aggccgagga tggcgccgcg ggcgctcata tcccagtaag gggcgtagag
     2221 cccggagaac gcggggacga agtagatgcc gccgctgtcc ttcaccttcg ctgcgaagta
     2281 ctcggaatcg ggggcgtcgt cgaagaggcg cagattgtcg cgcagccact ggactgcggc
     2341 gccggtgatg gcgatggatc cctcaagagc gtaggtcgcc ttgccctctt caaggccgta
     2401 ggcaaccgtc gtcagcaggc cgttcttgga gcggaccggc ttgtcgccga tgttcagcag
     2461 catgaagcag ccggtgccgt aggtgttctt cgcttcgccg gggctgtagc atgtctggcc
     2521 gaagagcgcc gcctgctggt cgccgaggat gccggatacg gggatcttcg cgccgaaggg
     2581 gccgctctcg ggggtatagc catagacgta gctgctgggt ttgatcgcgg ggagcatcgc
     2641 ctcgggaacg ccgaggaagt ccatcatttc cttgtcccac ttcagcgtct cgatgttcat
     2701 gaggagggta cgggaggcgt tggtgacgtc ggtgacgtgg acgccgccgt tggggccgcc
     2761 ggtgaggttc caggtaaccc atgtgtcgat gttgccgaag agaacctcgc ccttttccgc
     2821 cttcgcgcgg acgccgggga cgttgtcgag aatccacttg atcttcgttc cggagaaata
     2881 ggggctgatg acgaggccgg tcagatcgtt gaccttcttg tcccagccgg gcttcttctg
     2941 ccattcggcg cagaagtcct gcgtcctcat gcactgccag acgattgcgt tgtagaccgg
     3001 cttgccggtg gccttctccc agacgacagt ggtttcgcgc tggttcgtga tgccgatggc
     3061 gccgatctcg tccgccttga ctccgttcgt gttcagtacc tcgcggatga cgtcctgcac
     3121 gcgggaccag atctccatcg gattgtgctc cacccagccc ggcttcggat agatctgctc
     3181 gtgctccatc tggtgcgaat tcttcggagt tccctcaagg tcgaaaacaa tggctcggct
     3241 gctggtcgtt ccctggtcta tggccacaac gtatttcttc tcggacatga cagtacctcc
     3301 tgttttgggt ttatcgaggc gtgagaacaa gttgctgaac cagttgggca tgatcgtacc
     3361 gcctccttta tttgcgatgc cgccgagacg tgaacgccac tatagcgcct cccggctgtt
     3421 tcgcgtcata cacgagctat gaacagagcc ttattcctgt agctttctca atcccctggt
     3481 ttcgtccacg acggcggcga gccggtcaag ccccgccccg acgctggcct ccaccgaggc
     3541 catgagggtt ccctccagaa cagggccgtc ggcgatgagt acgcggcctg cacgttctcc
     3601 aaggagatcc ttggccgtgc gcgccgcaag aacagcgctc cccaggtcgg ggatgacgag
     3661 aacgccatcg gatgtttcga gcagcccttc aagcgcagag agaatcgacg gaaccgaggt
     3721 tccgagttcg ccgtcgtcgg ttccgccgca tgcggcgata ccgacccgct cccctgccat
     3781 ctgcgaggcg attgccacaa tgccccgcgc agcttcggcg ctgtgggaaa cgacgagaat
     3841 ggcgttcact caggaactct cctttacgga gcgcagaatc gtcgagagaa tcaggtagat
     3901 ggacgtcgct ccgggatcct ggtgtcccgc cgaacgctcc ccgaggtagc ttgcccgccc
     3961 tttgcgggcg acaaggggaa ttgtcgactc catacccgac tgcgccgcag cgacgcccgc
     4021 ttcgagagcg ccgcaaagat caacgccgtt gtcggaggct tttttcaaag cctcgactgc
     4081 cggcgtcagc gcgtcgacca tggtcttatc ctgaagctgg gctttgccaa gggcaacgat
     4141 gcccttaagc ccctcttcca ttgcatcggc cagggagacg gtatccacat cctgccttcc
     4201 ggcgagtttc gtcgacatgc gcatgaaaaa cgttccgtag agaggtcccg aagcgccgcc
     4261 gacatttccc atcagggtca tgccgacatc cttgaaaaga gcgccgagat ctgtgtagga
     4321 tcctgcggag agcttgtcgt gaaccttgtg aaaaccgcgg ttcatgttga ttccatggtc
     4381 ggcgtcgcct atagccgaat cgagttcggt caggtattcc ttgtgcgccg ccatcgtctc
     4441 gccgatagcg tcgaacacct tgagcattcc ctggacatcg aggttcatct ctgcgcgcct
     4501 acctccccca gcgaagcccc gccgtctcga cgggagcgtc ccaaagggcc ttcatttctc
     4561 cgtcgagttt cagcaaggta atggagaaac cctgcatttc gaggcttgtg atatagttgc
     4621 cgacgagaga acgtgcaatg acgatcccct tccccttgag gaaattgaca agatcgttgt
     4681 atgcaaggta gagttccatc tgcggcgtgc cgcccatgcc gttgacaaag gcgacaactt
     4741 cgtcgcccgc tttgaacgga agatcgtcga cgatcgcctt ggccatcatc tccacgatct
     4801 ccttcgccgt tttcagcttc atgcgctcac ggccgggctc gccatggatt ccgatgccta
     4861 tttccatctc gtcctcgccg agctcgaagg tcggtttgcc cgcagccgga acggcgcacg
     4921 atgtgagcgc catgcccatg gagcgaacct gactgttgac cttttcgcag agcgccttaa
     4981 cctcttcgag cgaagcgccc gcttccgcct tggcgccgac gatcttttcg atcagaacag
     5041 tgccgccgac tccgcggcgt cccgccgtat acagcgaatc ctgaacggcg acgtcgtcgt
     5101 cgacgacgac ctgcgcgacg ggaatgccgt ccgcagcaag aagatcggcg gccatctgga
     5161 agttcatgac gtcgcccgtg tagttcttca cgataaagag gacgccctta ccgccgtcca
     5221 cggccttggc cgcctcgtac atctgatcgg gagtgggaga ggtgaacact tcgccggggc
     5281 aggccgcgtc gagcataccg taccccacgt accctccgtg aagaggttcg tgcccgctgc
     5341 cgccgccgga gaggatggca accttttttt ggggcgcgtc ggccctcagg acagccttga
     5401 ccgacgggtg gagcttcacc agaccgggat gcgccgcggc gagaccggcg agcgattcga
     5461 cgacgacgtc atccactttg ttgaggagct ttttcactaa acaacgcctc ctttcaagga
     5521 gcaggttcta ttccggagaa ccggcggagg aggcgtttgt gtttctcccc cgccggccta
     5581 atttcgaaaa gaaaaccgtg cgttacatca cgccgagagc gtgtgcgatg aagtaggaga
     5641 ctgcgccgcc ggtaagaggc gcaagcacgg ggatccaggc atatccccag tccgaatcgc
     5701 cttttccggc gatcgggagt acaaagtgcg caatcctggg gccgaggtcg cgggccgggt
     5761 tgatggcgta tccggtgggg ccgccgagtg ccataccgat aaccatgacg agccagccga
     5821 cgaggaaggg accgaaaccg ggggtaaggc cgccgagggc ttccttgacg ccggaggaga
     5881 agatgaacat gattggaaca acgaggaaaa tggtaccgat catctcagtg atgaagttgt
     5941 tgatggggtt gcggatcgcg ggtccggtgg agaagacggc cagcttgagc cccttgtcct
     6001 cggtggcttc ccagtggccg aggtaggcaa gccatacgac gacgcctgcg aggataccgc
     6061 cgccgacctg cgccagcatg gtcatcatcg catgcggcag ggtgtaggtt ccggcgagca
     6121 tcttggccag tgtcactgcg gggttgatgt cggcctgagg cgctccgagg gatgtcgcgg
     6181 cgaagacgcc cattaccact gccgcgcccc atccgatggt gatgtgcacc catccagcat
     6241 tctgtccctt ggacttgttg agcagcgcgc aggcgacgag gccgtcgcca aaaacaacca
     6301 gagtcatggt tccgaagaat tcacccagaa gattcgtcat cgcatttcat acctccttcg
     6361 taaaataaaa acaaagaacg gtagctcaga aacacacttt tacatcctta ctcaatgctt
     6421 ggcatccctc ctcacatacg gttccgcagg aacctcaggg cagatagagc aaagaatcaa
     6481 cggacgtacg ctcacaaatc tcccgatggt taaggggatt cgtgaaaacc ttaataggga
     6541 gactttaccg gagcatgtcg gcggcgaagg ggaaaatcat aaatcatgtc gcgctggtga
     6601 agatgataat acctttctaa cgaaagatat ccacggcgtc cactacgctt cgccattcct
     6661 tatttgtccc ggcagggctc cggggaaatc gctccttgcc gcacaccacc gcttgaagcg
     6721 acgaaatgcc gcgaaaggat tgggttgtgt taccggagcc gacacggtct tctgattctc
     6781 gcgcaatctt cggaagtatg ttgctgcttc acccgtgcga tgatatacca tttttcgcca
     6841 cattgtcaac gagtgaaata aaaaaaagga aattgtaaat gagctaacat aatttacggt
     6901 atacatttta atgtaagtat gtgcggatac gcctctaact tgtacaaaat gcatgatgtc
     6961 tcttcgattt gacgagcgct cacgtccagg aaaagaaact acccgacgaa gcctcttgac
     7021 aaagagggcg cccccgctta tcatttcgtc atgtgtaaaa ttaacgaaat aaaggagtca
     7081 gctatggaat ccatactttc catttcaaaa gcgctcggag aaaagaacag gctccgcgtc
     7141 tttctggcgc ttatccacac gaaggaactt tgcgtctgcg aggtggggga acttctcggc
     7201 cttgcccccg cgacagtgtc gcgccacatg agcattctct cccgggcaaa gctcgtcgac
     7261 tcccgtaaac gaggcaaatg ggtctattac tgtatcaagc ctgcgatagc gccggagtta
     7321 ctccaatgga taaaaaattc cgtcgtcgat tctttttccg ttgcggaaga cgtgaagaga
     7381 atcgagatcc tcgtcaggga acgccaggac aaagactgta caaaagcctg tgtatccgaa
     7441 gagaggtgaa ataacgtgag caaaaagcca aaggtgctct ttctctgcac cgggaactcg
     7501 tgccgcagcc aaatggccga ggggtggacc cgtcatttcc atagcgccgc catagaagcg
     7561 cattctgccg gaatcgagac gcacggcctg aatccgttcg ccgtggaggc tatgaaggag
     7621 gcgggagcgg acatctccgg acagcgctcc aaaaacgtga tggagctgct cgacgtttca
     7681 ttcgactacg tcgtcaccgt gtgcggcgac gcggacgagc ggcgcccgtt ctttccgggg
     7741 gagacgagag tcctccacgt cggcttcgac gacccgccga agctggcgct cctcgaagag
     7801 acggaggagg gaaaaatgaa ctgctaccgc cgggtgcgcg acgagatacg cgcattcatc
     7861 aaagaccttc ccggctttct tgaaaatcta ccggaggaaa aaaggagcta gatcaagtat
     7921 ggacgatatt cgcaactacg tgaaagagcg gtacgcgcag gcgatcaaga acaagtcctc
     7981 atgttgcggc tcgggagggt gctgctgcgg aacgacgggc gcatccggca tcccgagcat
     8041 gacgcgcggc aactacgacg gcgcgacgct cgaaaaaact ccggagcaga tggccgacca
     8101 gtccttcggc tgcggcaatc cgttcaccga ggcgcgcatc catccgggcg agaccgtcct
     8161 cgacctggga agcggcgcgg ggctcgacct ctttctggcg tcggagatga ccggcccgta
     8221 cggcaaggtc atcgggctcg acatgacgga cgagatgctt gcgaccgccg ccgaaaatct
     8281 caaggggatc gaaaacgtct ccctggtgaa agggtacatc gaggagatgc cggtcgagag
     8341 caactccgtc gatgtcatca tttcaaactg cgtgatcaac ctttccccgg acaagggaaa
     8401 ggtgctgaag gaggctttcc gcgttctccg tcccggcggg aggttctgcg tctccgacac
     8461 cgtcttcctc cgtcctgtga cggagcgcgc cgtcaagaac ctggcggcgt ggtccgggtg
     8521 catttccgga gcgctccggg agacggagta cgcggagaag atgagggagg cgggcttcga
     8581 ggaggtcgaa gtacggcgca cgaaggtgta ctccttcccc gacgcaatgg cggcgatggc
//
LOCUS       DAOPKU010000048         8634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8645_length_8634_cov_5.405292, whole genome shotgun sequence.
ACCESSION   DAOPKU010000048 DAOPKU010000000
VERSION     DAOPKU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8645_length_8634_cov_5.405292"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK849_02530"
     CDS             complement(<1..523)
                     /locus_tag="PK849_02530"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPE65030.1"
                     /translation="MFSPFSYEMPTRVEFGRGTALKVADEVRRLGCSRVLLLSDNGVK
                     NAGLLDGVQRALHSGSVPCELFLDIEPNPKDRNVAAAAQAARAFKADCLVAVGGGSPI
                     DCAKATAIVAAAGGTARDYEGRFKSDIPPLPIIAIPTTAGTASELTFGAVITDTEAKF
                     KFTAKSTQLAPKVA"
     gene            complement(566..1879)
                     /locus_tag="PK849_02535"
     CDS             complement(566..1879)
                     /locus_tag="PK849_02535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE65031.1"
                     /translation="MISTSRRMELEKELLEQFHLQEQIFSLVPVPLVMKDANSVYLHV
                     NQAFADFLGLPREQVLGKGPFDLFSPEFAQRIFEEDQEIIRNGVPATDLERRIKNGHG
                     KMVWLKSYKGPVFNSSGGVIGIVGGNMDITAVKNAEEALRKSEERWKFAVEGAGDGVS
                     EWNLETGDVFYSKQWKAMLGYKDDEIAPTFEEQKKRIHPDDLSDVMFALEQHLRGETP
                     SFSHEFRMATKNGGWIWVLDRAKVIEWSDDGAPLRMIGIYTDITRRKSAEEMILHQAT
                     HDMLTGLPNRMLFNDRLFMAMADGKRLGRKTALIFLDLDNFKTVNDTLGHQMGDLLLM
                     AVAERLSKEIREIDTLARFGGDEFAFVIPFLENKEGAAALADRIQQSLCREFRIDGKS
                     LFITGSMGVAFHPDDSDDLETIMKQADMAMYRSKREGRNAWRFWG"
     gene            2244..3536
                     /locus_tag="PK849_02540"
     CDS             2244..3536
                     /locus_tag="PK849_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE65032.1"
                     /translation="MIQRHFKDVCCDILILGGGIAGAAAALAAAREGASVVLAEKSGR
                     LGGIATNGLVSTLCGCYSSLPGRRKIIGGFRDLLAAELHRTGGILEGVDRDTHRNDTC
                     DPELLACALDTLMQRHDVQLLFSSCVIGADFSERLLRSVEVVNTESGTRCSITPRLAV
                     DASGGGLLSRLADPASIVSGGEGGKLQAATMVFRMDGVDGDRARLVTKDEIRALLAEA
                     RRNGDVVGERGNGVLTVLPGNSSAIVNANWVQADMSVPEDVTRGLVEGRKKALENARF
                     FRKYVPGFGNAVLSSIAASLGVREASRIKGKYVLSQEDVMEGRRFEDAISLGSWPMEY
                     HDYEQNRLVYLWSENDYDIPFRCLLPEGLDNVIAAGRNISVSSMAYASTRVMGPCMAI
                     GQAAGTATRLCLKMNALPKEVDIHLLRKTLLNEGAILE"
     gene            complement(3577..5085)
                     /locus_tag="PK849_02545"
     CDS             complement(3577..5085)
                     /locus_tag="PK849_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPE65033.1"
                     /translation="MDLVLSVVSQIDAVFVFFIFAGAVAGLFVGAIPGLSVTMATALL
                     VSITYTWSVKNALGVILGVFVSGVYAGAVSAILINIPGAPSSVVTTLDGYPMARKGEA
                     ALALWAATFHSFVGTVFGFLVLMFVAKPVTSFALTFSAMDYFLLALFGLTTIGTLTAK
                     NYIKGGICALLGLLLSMIGIDPFSGGVRLTFGWFDLQGGIPLIAALIGLFGFSEVLDQ
                     VARGAMNAIAAEAGKMRYCFREMLRFLPLSLRSSLIGVFIGALPGAGGPVASIIAYDS
                     ARKSVKNPSRPFGEGAVEGVIASEAANNACIGGALIPMLTLAVPGDAVTAVILAAFYV
                     HGLQPGPLLFVRTPELFSIVLAGGFIASVAMLILGMTVVPRLSKIVLVPKRILMPLVT
                     VLCVVGTYAVNSSMFDVGLMFCFGLAGFLMRSRGYPPAPLVLGLVLGEMMDSNLRRAI
                     NMALAGDNFFLSLFGRPTSVILAVLVILSVLAAVPGVKDRVLSVFSRRERVE"
     gene            complement(5098..5553)
                     /locus_tag="PK849_02550"
     CDS             complement(5098..5553)
                     /locus_tag="PK849_02550"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HPE65034.1"
                     /translation="MNTSPWAANMRHSLFFLVLAALFGWQGARSHDGGDWALSPALFP
                     LLLSSALALLALALLLQGYRRLKNGVPAVLPQGADFSTGRVFLVFGLCCAYALLLPWA
                     GFFPSTALFLVIFSLMIGERRLWPIVLLSFLSPAAILLIFRHGLNVLLP"
     gene            complement(5631..6644)
                     /locus_tag="PK849_02555"
     CDS             complement(5631..6644)
                     /locus_tag="PK849_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HPE65035.1"
                     /translation="MKKVWKLGVLVFIVAAMLSVPAGGFADEYPSKGITLVVPWGAGG
                     VTDVAARVFAPLFEKYMGVPVVILNRPGASGAIGTEYAYARPADGYTVLFSAETPASF
                     RIMDVSKLSFHDFEPIMLLSHSEKIIAVAPESPYQTLADLVNDIRARPGKVRFAYSGA
                     GASGHVQGLLMMQKGNLDFSSTPFGGGNNALLAVLGGQVDFTSPNIGTVKDYISSGKL
                     RALAVFDSKKTAYLPELPPITDAIPEIAPYLPLDYPNCLAVKKGTPPEVVEKIADATS
                     KAVADPKWKEFLENNWYVGLDAVRGQDMLVYWDKWASVVGWVLQDAGVAKKSPEAFNI
                     PRP"
     gene            complement(7020..8339)
                     /gene="xylA"
                     /locus_tag="PK849_02560"
     CDS             complement(7020..8339)
                     /gene="xylA"
                     /locus_tag="PK849_02560"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF003998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HPE65036.1"
                     /translation="MGDLFPGIKKIQYEGPLSQNPLAFKEYNADEVVAGKPMKEHFRF
                     AMSWWHTICSSGSDMFGSGTAIRPWDYEPHPVKRAKLAADGAFEFMEKLGLEYFCFHD
                     RDIAPEALTLAETNERLDEVCDYVKGLMSKTGIKLLWGTANLFSHPRYMHGAATSCNA
                     DVFAYAAAQVKKAMTLTRDMGGVNYVFWGGREGYETLLNTNMSLELDNLARFLKMAVA
                     YAKEIGFTGQFLIEPKPKEPTKHQYDYDVPAVLAFLRKYGLEKDFKINLEANHATLAG
                     HTFHHELHIARINGVLGSIDANQGDLFLGWDTDNFPTDVNAATLAMVEVLKNGGIAPG
                     GFNFDAHVRRPSFQDDDLFIAYIVGMDTFAKGLKVAAKLIEGGEIEAFVAKRYESYSS
                     GVGKKIADASVGFRELEAYALAHDQPVNGSGRQEMLEAIVNRYMLNP"
     gene            complement(8424..>8634)
                     /locus_tag="PK849_02565"
     CDS             complement(8424..>8634)
                     /locus_tag="PK849_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65037.1"
                     /translation="NPDFIMLGGMGSILPPSFVAGVGENVAKKTPGATSPDFLLTTAS
                     LGIESAALEGATLMAMNHYVKKAVK"
BASE COUNT         1901 a         2558 c         2427 g         1748 t
ORIGIN      
        1 gcgcaacctt cggagcgagc tgcgtgctct tcgcagtgaa cttgaacttc gcctccgtat
       61 ccgtgatcac ggcgccgaac gtcagctcgc tcgccgtccc ggccgtggtc ggaatcgcga
      121 tgatcggcag cgggggaata tcgcttttaa aacgtccttc gtaatcccgc gccgttcctc
      181 ctgcggccgc cacgatcgcg gtcgcctttg cgcagtcgat aggactgccc ccgcccaccg
      241 ccacaagaca gtcggctttg aaagcacgcg cggcttgggc cgcggcggca acgttgcgat
      301 ccttgggatt cggctcaata tcgaggaaca gttcacacgg cacggagccg gaatgcaagg
      361 cgcgctgtac gccgtcgagc agacctgcgt ttttcactcc gttgtccgaa agaagcagca
      421 ccctggagca ccccaggcgt cgcacctcat ccgcgacttt caacgcagtt ccgcgtccga
      481 actctaccct cgtgggcatt tcataactga acggtgaaaa cattctctct tcccctttca
      541 catgtgaaca gactgcacga aacgctcagc cccagaagcg ccatgcgttc cgcccttctc
      601 ttttcgaccg gtacatcgcc atatcggcct gtttcataat tgtctccaga tcgtcgctgt
      661 cgtcggggtg gaaggcgacc cccatgctcc ccgtgataaa aagagacttg ccgtcgatgc
      721 ggaattccct gcaaagactc tgctgtatgc ggtcggcaag ggcggcggcg ccctctttgt
      781 tttcgagaaa cggtatgacg aaggcaaatt catcgccgcc gaacctggca agagtgtcga
      841 tctccctgat ctctttactc aatctctccg ctaccgccat gaggagcaga tcccccatct
      901 gatggccgag cgtgtcgttg acggtcttga aattatccaa gtcgaggaag atgagcgcgg
      961 tttttctccc gaggcgcttt ccgtccgcca tcgccatgaa gagcctgtcg ttgaagagca
     1021 tgcggttcgg gagtccggtg agcatgtcgt gcgtcgcctg gtgaaggatc atctcctccg
     1081 cgctcttccg cctcgtgata tcggtgtaga tgccgatcat tctgagcggc gcgccgtcgt
     1141 cgctccattc gatcacctta gcgcggtcga ggacccaaat ccagccgccg tttttcgtgg
     1201 ccatacggaa ttcgtgggaa aaagaagggg tttccccccg cagatgctgt tccagcgcga
     1261 acatcacatc actgaggtcg tcggggtgta tgcgcttttt ctgttcctcg aaggtcggcg
     1321 caatctcgtc atccttatac ccgagcattg ctttccactg ttttgaataa aacacatcgc
     1381 cggtctccag attccactcg ctgactccgt cgcccgcccc ctcgacggcg aacttccatc
     1441 gctcttcgct cttgcggagc gcctcttcag cattcttgac cgccgtaatg tccatgttgc
     1501 ctccgacgat accgatcacg ccgccgctgc tgttgaacac agggccttta tacgatttca
     1561 gccataccat ctttccgtgg ccgttcttaa tacggcgttc cagatcggtc gcgggaacgc
     1621 cgtttcgtat gatttcctga tcctcttcga aaatgcgttg cgcgaactcg ggagaaaaaa
     1681 gatcgaaggg tcctttacca agcacctgtt ccctgggaag cccaagaaaa tcggcgaacg
     1741 cctgattgac atggagatag acggaattcg catctttcat gaccagggga acgggaacca
     1801 gggaaaaaat ttgttcctgc agatggaact gctccaatag ctccttctcc agctccatcc
     1861 ggcgagacgt tgaaatcaaa ggttctccgg ataaagactc gaattccttt tttgtcttct
     1921 gacgaagagg agccggtcct tttttttgaa gcaccatgtc cggtgtggta ctttttttgc
     1981 aggacactga ataatttccc ccttgtgtgt ggaaacgatg caaaaaaccc gggtatttct
     2041 cgggatttct caagtctgcg gagtcgcgtg aatgaaacaa tactatcaca aagggggaaa
     2101 caaaaaaata atttgcctga ttctctgaaa aaagttttct gagattgctt cgcgtacgtc
     2161 gtatccgtta cacttaacct ctcgactgaa gaatcgccgc ttcctgtaaa actgttcgga
     2221 agcgaagaaa tgaggaggcc ataatgattc aacgccattt caaagacgtt tgctgcgata
     2281 ttcttatcct gggaggcggc atcgcaggtg cggcggcagc tttggcagca gcccgcgagg
     2341 gagcgtcggt cgtcctggcg gaaaagagcg ggcgacttgg cggcatcgcg acgaacggtc
     2401 ttgtgagcac gctctgcggc tgctattctt cactgccggg aaggcgcaaa atcatcggcg
     2461 gattccgcga tctcctggct gcggagcttc atcgtacggg aggcatactg gaaggggtgg
     2521 acagagacac gcacagaaac gacacctgcg atccggaatt gctcgcgtgc gccctcgaca
     2581 ctctgatgca gcgtcacgat gtgcagctgt tgttctcctc atgcgtcatc ggcgcggact
     2641 tctcggaacg ccttcttcgt tccgtcgagg tcgtcaacac ggaatcgggg acgcggtgtt
     2701 cgataactcc ccgtctggcc gtcgacgcct ccggaggagg cctcctctcc cggctggccg
     2761 acccggcatc catcgtctcg ggcggcgaag gaggcaaact ccaggctgca accatggtct
     2821 tccgcatgga cggcgtcgac ggagacaggg ctcgtctcgt caccaaggac gagatacgcg
     2881 cgctgctcgc cgaggcccgc cggaacggcg acgtcgtcgg ggagcgcggc aacggcgtct
     2941 tgacagtgct tcccggcaac agctcggcca tcgtcaacgc caactgggtg caagccgata
     3001 tgtcggttcc ggaagatgtg acgcgcgggc ttgtggaagg ccggaaaaag gcgcttgaga
     3061 acgcccgttt cttccggaag tacgtcccgg gcttcgggaa cgcggtcctc tcctccatcg
     3121 cggcttcact tggcgtgcgc gaggcgtcgc gtatcaaagg caagtacgtc ctctcgcagg
     3181 aggacgtcat ggaggggcgg cgcttcgaag atgcaatttc gctgggctcc tggcccatgg
     3241 agtatcatga ctacgaacag aacaggctcg tctacctgtg gagcgaaaac gattacgaca
     3301 ttccgttccg ctgtctgctc cccgaggggc tggacaacgt catcgcagca gggcggaaca
     3361 tctcggtatc gtcgatggca tacgcctcca cccgagtgat gggaccgtgc atggccatcg
     3421 gccaggctgc gggaacggcg acgcgcctct gcctgaagat gaatgcgctc cccaaggaag
     3481 tggacataca cctgctccga aagacgcttc tgaacgaggg ggcgattctg gaataatgaa
     3541 ggggaggctg gtcggcctcc ccttcattat tctgctttat tctacccgct ctcttcttga
     3601 aaaaactgag agcacccggt ccttcactcc cggcacggcg gcgagaacgg agagaatcac
     3661 taaaaccgcg aggatcacgc ttgtcggtct gccgaagagc gagaggaaaa agttatcccc
     3721 cgccaacgcc atattgatcg cacgccgaag attcgagtcc atcatctccc ccagcacgag
     3781 gccgaggaca agcggcgcgg gcggataccc ccggctccgc atcagaaacc ccgccagtcc
     3841 gaagcagaac atcaatccca cgtcgaacat gctggagttc accgcatagg tcccgaccac
     3901 gcagagcacc gtcacgagcg gcatcagaat tcgcttgggg acgaggacga tcttcgagag
     3961 ccgcgggact acggtcatcc cgaggatcag catggcgacg gaggcgatga atccccctgc
     4021 gaggacgatg gagaagagtt caggcgtccg gacgaagagc agcggtcccg gctgcaatcc
     4081 gtggacgtaa aaagccgcga ggatgactgc cgtcaccgcg tctcccggca ctgcaagcgt
     4141 gagcatcggt atcagcgccc ctccgatgca ggcgttgttc gccgcttcgc tcgcgatgac
     4201 cccttccacg gcgccttcgc cgaacggacg gctcgggttc ttcacgctct ttcgcgccga
     4261 gtcgtaggcg atgatcgaag cgacgggccc ccccgctccg ggcagcgccc cgataaagac
     4321 cccgatgagc gaggaacgaa gcgaaagcgg gaggaagcga agcatctcgc ggaagcagta
     4381 ccgcatcttc cccgcctccg ccgcgatggc gttcattgcg ccgcgcgcaa cctggtcgag
     4441 cacctccgaa aacccgaaga gccctatcag ggcagcgatc agaggaatgc ctccctgaag
     4501 atcgaaccac ccgaacgtca gacgaacgcc gccggagaag gggtcgatac ctatcatgct
     4561 caacagcagt cccagaagag cgcagatgcc ccctttgata tagttcttcg ccgtcagcgt
     4621 gccgatcgtg gtcaggccga aaagcgccag cagaaagtag tccatcgcgg aaaaagtaag
     4681 cgcgaagctc gtcacaggct tggcgacgaa catcaggacg agaaagccga agacggtgcc
     4741 gacgaaggag tggaaggtcg ctgcccagag agcgagcgcc gcctcgcctt tgcgggccat
     4801 cggatacccg tcaagcgttg tcactaccga ggaaggagcc cccggaatat tgatcagaat
     4861 ggcggagacc gcgcctgcgt acaccccgga gacaaagacc ccgaggatga ctccgagcgc
     4921 gttcttcaca ctccatgtat aggtaataga cacaaggagc gccgtcgcca ttgtcacgga
     4981 aaggccgggg atagccccca cgaagagccc cgctacagcc ccggcgaaaa taaagaacac
     5041 aaagacggcg tcgatttgag aaacgacgga aagcacaaga tccattgatt cttcctccta
     5101 tggaagcagc acgtttaaac cgtggcgaaa gatgaggaga atcgccgccg gagaaagaaa
     5161 cgaaaggagc acaataggcc aaagacggcg ctcaccgatc atcagggaga aaatcacgag
     5221 aaaaagcgcc gtagagggga agaagcccgc ccagggaagc aagagagcgt aggcgcaaca
     5281 taagccgaaa acgagaaata cccttcccgt cgagaaatcc gccccctgcg gcaataccgc
     5341 aggcacgcca ttttttagtc ggcggtagcc ttgcaggagc agcgcaaggg cgagtagagc
     5401 aagcgccgac gagagaagca gaggaaaaag cgcgggagaa agcgcccagt cgcctccgtc
     5461 gtgcgagcgc gctccctgcc aaccgaaaag cgcggcaagg acgaggaaga acaggctgtg
     5521 ccgcatattt gccgcccagg gagaagtgtt cataaaaagc catcctttca tgaaagcaaa
     5581 agagccgcca ctcccttgcc gggaaggggc ggctcgtcgt gaagcgagtt ttacggtctg
     5641 ggaatattga acgcttcggg gctctttttc gccactccgg cgtcctgcag cacccatccc
     5701 accacggagg cccacttgtc ccagtagaca agcatatcct ggcctcgcac agcatccagc
     5761 ccgacatacc agttgttctc caggaactcc ttccatttcg ggtcggcaac agctttcgac
     5821 gtcgcgtcgg cgatcttctc tacaacctcc ggaggagttc cctttttcac cgcaaggcaa
     5881 ttcgggtagt caagcggcag atagggggcg atctcaggaa tggcgtcggt gataggaggc
     5941 agttcaggca ggtaggcggt cttcttgctg tcgaagaccg caagcgcgcg gagtttccct
     6001 gaggagatgt agtccttcac agtcccgatg ttgggggacg tgaaatcgac ctggccgccg
     6061 aggaccgcga gcagcgcgtt gtttccgcct ccgaagggag tgctcgaaaa gtcgaggttt
     6121 cccttctgca tcatcaggag cccctggacg tgaccgctgg cgcccgcgcc gctgtaggcg
     6181 aagcggactt ttccgggccg cgcgcggata tcgttcacca ggtccgcaag cgtctggtac
     6241 ggcgactccg gcgcgaccgc gatgatcttt tccgaatggc tcagcagcat gatcggctcg
     6301 aagtcatgga aggagagctt actcacgtcc atgatccgga acgacgcagg cgtctcggcc
     6361 gagaagagca ccgtatatcc gtcggcagga cgggcatagg catactctgt gccgattgcg
     6421 ccggaagcgc cgggccggtt gaggataacc acggggacgc ccatgtattt ctcgaagaga
     6481 ggcgcaaaga ccctcgccgc cacgtcggtg acgcccccgg ccccccaagg aactacaagc
     6541 gttatcccct tcgacggata ctcgtctgca aaaccgccgg ccgggacgga aagcatcgcc
     6601 gctacaataa agaccaggac gcccagtttc cacacctttt tcatctgcaa cactcctttt
     6661 gttctttcgt ctttctccat agagaagatg agaggaatac aaccgtttca tccaatgaac
     6721 gcaacgcgtt tcggttgagg gtgagcgact tccacccgcc aaaacataac ttaggtacac
     6781 taccgatcaa aagaatgaat aacggttgaa ggcattataa actatcaaca tactacctta
     6841 caagagcatc aggaagaaga gaaaactcgc cggaactaaa aaaccgtacg ccgatggaca
     6901 ttccggttgt gctgccggca gcgctccgtg aaaaaaaccg accggttctt cagcttcgcg
     6961 aaagatgaac cggtcggaaa cgactccatt tctggatttt ctacgagccg tatggttgtt
     7021 tacgggttga gcatatagcg gttcacgatc gcttcgagca tctcctgccg tccggagccg
     7081 ttgacaggct gatcgtgcgc gagcgcgtac gcttcaagct cccggaatcc gacggaagcg
     7141 tccgcaatct tctttcccac tccagacgag tagctttcgt accgttttgc cacaaacgcc
     7201 tcgatctcgc cgccctcgat cagtttggcc gcgaccttca gccccttggc aaaggtatcc
     7261 atcccgacga tgtacgcgat gaagagatcg tcgtcctgga acgaaggacg acggacatgc
     7321 gcgtcgaagt taaagccgcc cggagcaata ccgccgttct tcaggacttc gaccatcgcg
     7381 agcgtcgccg cgttcacgtc cgtcgggaag ttgtcggtat cccatccgag gaagagatcg
     7441 ccctggttcg cgtcaatgct gccgagaacg ccgttgattc tggcgatatg cagttcgtga
     7501 tggaaggtgt ggcccgcgag cgttgcgtga ttcgcctcca gattgatctt gaaatccttc
     7561 tccagaccgt acttcctcag gaatgccagt acggcgggga cgtcatagtc atactgatgc
     7621 ttggtaggct ccttcggctt cggctcgatg aggaactgtc ccgtgaaacc aatctccttc
     7681 gcgtaggcta ccgccatctt gaggaagcgg gccagattgt cgagttcaag gctcatattg
     7741 gtgttcagca gagtctcgta cccctcccgg cccccccaaa agacgtagtt cacaccaccc
     7801 atgtcgcggg tgagggtcat ggcctttttg acttgggcgg cggcataggc aaagacgtcc
     7861 gcgttgcacg atgtggcggc gccgtgcatg tatcgcgggt gactaaagag gttcgcagtc
     7921 ccccaaagga gcttgattcc cgtcttgctc atcagcccct tgacatagtc gcaaacttcg
     7981 tcgaggcgct cgttcgtctc ggcaagcgtc aacgcctcgg gagcgatatc cctgtcgtga
     8041 aaacagaaat actcgagccc cagcttctcc atgaactcga aggctccgtc cgctgcgagc
     8101 ttcgcccgct tgaccgggtg cggctcgtaa tcccacggac ggatagcggt tcccgagccg
     8161 aacatatcgc ttcccgaaga gcatattgtg tgccaccatg acatggcgaa gcggaagtgt
     8221 tccttcatag gctttccggc gacgacttcg tcggcgttgt actccttgaa agcgagcgga
     8281 ttttgtgaaa gcggcccctc atattgaatc ttctttattc cggggaagag atctcccatt
     8341 gtatttacct ccttttcaga gtgcagtttc tttttccttg acgccctcac acgagaacat
     8401 ctccttggat tcgtcgcctt tcttcatttc actgctttct tcacataatg gttcatcgcc
     8461 atgagcgtcg ctccttcaag cgccgcggat tctataccca atgaagctgt ggtgagcaag
     8521 aaatccgggg aagtcgcgcc gggcgttttt tttgcgacgt tttctccgac acccgctaca
     8581 aacgaggggg gcagaatgga tcccattcct ccaagcatga tgaagtcggg atta
//
LOCUS       DAOPKU010000049         8617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8665_length_8617_cov_4.540762, whole genome shotgun sequence.
ACCESSION   DAOPKU010000049 DAOPKU010000000
VERSION     DAOPKU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8665_length_8617_cov_4.540762"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PK849_02570"
     CDS             <1..783
                     /locus_tag="PK849_02570"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPE65038.1"
                     /translation="VLARLAVLRGDGEAYATARKSLCGYRKEFPERSVVRMVELCLAS
                     LDLLLGRTEGIPDWLRDVESIKKTLYIQGHACGLILYGKLLLLEKRFPELYGLTEPAM
                     EFAYGMNYELPRLCQHICLAAAYTEEGREDEAENHLSRALRIALPDRIYLPFAELGTT
                     LLPLLENAKAGFNMKQMNSLIALCKRHSSGAASVRRHLDPSTALLTPREREIALLAKE
                     RCSTREIASRLFISENTVKSALKIIYGKLEVHSKNELATMDF"
     gene            901..1188
                     /locus_tag="PK849_02575"
     CDS             901..1188
                     /locus_tag="PK849_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65039.1"
                     /translation="MRVQTGYIRSVKALPGYKLEVEMTTDTRIEFDFASRLNTIRFGA
                     LKDEKLFVTASTDGNFITFGTNDAVKIRISAIDFMDLVLVDRTGRFPPDET"
     gene            1354..1824
                     /locus_tag="PK849_02580"
     CDS             1354..1824
                     /locus_tag="PK849_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65040.1"
                     /translation="MKKTLVKTAGLFVLAALLAVHCASRAEAWIKITLKNNRSHNIAV
                     AFCWSGFDYADDQRKGWYVVQPNDSRVINLANATSSLTWEDFGFYAQGTLRNGKKIRW
                     SGDMKQVIIHPSKAFDGHPEDPIAGGKKVGFRKLNLKKVGDANTDAVATLTFNP"
     gene            1839..2021
                     /locus_tag="PK849_02585"
     CDS             1839..2021
                     /locus_tag="PK849_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65041.1"
                     /translation="MQLWAAALLFVLFAAAAFSFLKGGRNKSGIRFYTGILFAFIGGV
                     ALAYIAATVFFVVSIE"
     gene            2115..3227
                     /locus_tag="PK849_02590"
     CDS             2115..3227
                     /locus_tag="PK849_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65042.1"
                     /translation="MFHCTQKRLVTIMVVCSFLVFSCGAALAAADKGYSSADLKKMST
                     FLSNFTELGFMSFDAKELTNEKDPADMIRFGIWHNYVNNFKSRISQCKKKDCEWGSLV
                     IEGKYVAETIKKYFDVDYKKLASVTESDPPYFYDGKLYHFEGADGEAVYYARVSEAEK
                     NTKGQIVMRGEIYNAEDENDLLGSFEALAKPHKFGGKDTWAILSMETEYYDNEGQVSD
                     EAVEGLWIEVPNFPAEAEVVDFQMNDEGVASFTRILDAGVVALVVERLPVTNTEGEQV
                     TPQTLGKLVAEFEGIEEEKIEITPSLDDFAKLYSYPVAGAEFTTGENEDTRKVIDLFL
                     FTDSWLFRIHVSVTADSFEEYEDQVKEWLSSLKFRE"
     gene            3523..>8617
                     /locus_tag="PK849_02595"
     CDS             3523..>8617
                     /locus_tag="PK849_02595"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPE65043.1"
                     /translation="MRKFFVALLAIVLLFGGAYGAWAADDFTILSFSPGGMVKGRMPV
                     KIAFSQPVVEKSLVGKTAPQQHIPVEFSPSIRGAGKWTDQKTFVFTPVANLQQATRYR
                     ATAKESLQDAKGRRLSGKQSFEFFTEPLKLLRAQQIDFTESNNVVIELKFNLPVSPFR
                     LRGFLSLLNEQRQQLGYSPAGQAPSSTVRLTTNSFEGGKIALVLSPGLTSDAGPLGIE
                     QEIRHEIAVTRKLEIRSVYADTRYPATSVINVNTSVGADMSVVSKFIEISPATEISID
                     PGYNGFTILGDFKPKQRVELTIKKGLPTKEGKALEQEFKRAVIFPDYYPSLAFPAGGT
                     FLSPAGDLRIPIESVNIEEITLNLWRLYENNIPVSMTMSNYSIPRDLARRVATRTARP
                     EGDLNETVRRAIDLRELAGEAKGVFLLTASESSGEYWADTEQLVAVTDLGIVARVWAN
                     GITVWVNSILGIEPVKDATVRVYSKSNQLLASGSTDENGLWTKQGAEPWDDQLLPAIV
                     TVEKGDDISFLKFESNLLADTGFDIDGRPWTDKYDALLFAPRGVFRPGENVDFSALVR
                     TKDLLPPEPFPVVYVVRSSLGREVARGTALLSSEGIASFSARLAPASPTGTYSAVIAL
                     PGGENAPLGRTEFFVEDFVAPRLEVKAVPDASFLTAGDTLTIDVSSSYLFGAPASGLV
                     FEGEVRAVAAPFRPEGWNAFTFGDMERTFESMTDFVGSGTLDETGKGTLSFSGPEGWL
                     PPAAIDLSFFVRVMEESGRWVPTSVRLPFHPYPVYLGIETPKGDIVPGKEIAVRVAAV
                     TPEGKASDLEIAVAEVFLVQRHYNLVRTGNQTRMQMQRELVPHAQGGVEFADGIGSFS
                     FTPKIDGEYVVKISDETSNSMASSTLYAWTPYGGGASGSTLLDRVVVTADKKSYEPGE
                     KATVTLRSPFEGNLLVTVETNRELFRKVVSLKKGEVNLQVPVTDDMIPNAYITASVIR
                     PVVEGEEWGNHRAIGTLSIPVERKEYHLDIALLSPERTLPDEPLKVNGTLTDGSGAPR
                     KGEVVLFLVDEGILSLTGYRTPDPWAFFMAKRAQGVSVYDLYDQLLPLESRETPLLKA
                     GGGAGEEAMAALRAGMSPVSARTFRMLSLFLGSVPTDENGAFEATFDVPEFSGKGRLM
                     AVASAGKSFGKGEKFVAIARDITTELSLPRAVSPGDTFHAPLKIFSSASGEKQVSVKV
                     GIDGPLALTGKKEFVANLAAGGEALFSVPFKAGDEAGTTYLTVETQWEDGSFTQNLDL
                     PVRPAFPRIALSGSGVVRGGGQGSIEIPRDWFPGTEEGRLLLSDLPMLDLAGAASFLL
                     QYPYGCLEQTVSGAWPLLVLGDIVRDVDPLLVNEEEQKSALALRIRQISAMQLYSGGF
                     AMWPGSSIPSMWGSLYAAHFLVEAKKGGAPVPDDLLDSALANVRSMLPLAPANESDVA
                     YRENMTLKAYAAYILALRGEPPLGWMAHLNEHSDALRESGKIFLAGAYALASKTSEPL
                     RKLGTGAPSLRGGESTTFESASRNNALKLLMWSEVDPLSATASDLALKLIEEARSSTW
                     RTTQDNGMAVLALGRWMEKTRGARKPFTALVKDTSGATVASFTDGKRVSLSLKEVPQG
                     PLTLSLDGEGTAYFAWTSAGVPMKAPKPSSNGIALKRTWSGRDGKPLAEGAPLSRGDR
                     ITATLTMTPSTALSDLVVVDMLPGGMEIEN"
BASE COUNT         1918 a         2490 c         2529 g         1680 t
ORIGIN      
        1 gttcttgcgc gccttgcagt tctgcgaggc gacggagagg catacgcgac ggcccgcaag
       61 agcctgtgcg gataccgaaa ggaatttccc gaaagatccg tcgtccgcat ggtcgaactc
      121 tgccttgcct cgcttgatct cttactggga agaaccgagg gaatccccga ttggcttcgc
      181 gacgttgaaa gcatcaagaa aacgctctat atccagggac acgcctgcgg cctcatactc
      241 tacgggaagc tgctgcttct ggaaaagcgt tttccggagc tgtacgggct gacggaaccg
      301 gccatggagt ttgcgtacgg gatgaactat gagctccccc gcctctgtca gcatatctgc
      361 ctcgccgccg catacacaga ggaaggacgc gaggacgagg cggagaatca tctttcccgg
      421 gcgctccgga tcgcgcttcc ggaccggata tacctgccgt tcgcggagct cgggacgacg
      481 cttctgcctc ttctggagaa cgcgaaagcc ggcttcaata tgaagcaaat gaactccctg
      541 atcgcgctct gcaagaggca ttcttccggc gccgcatccg tacgaagaca ccttgatccc
      601 tccacagctc ttctgacgcc gcgggaacga gagatcgccc tgcttgcaaa agagcgctgc
      661 agcacccgcg aaattgcatc ccgtctgttc atttcggaga acactgtcaa atcggcgctc
      721 aaaatcattt acggcaaact ggaagttcac tcaaagaacg agcttgccac gatggacttc
      781 taacgagcgt agaacaccac cccccgttcc cccccgaaag agggggtgta gaacaagcag
      841 ttttccttca aaatacttat ggactctgcg ggaaaggctc ttctacaggg gtgtgagaaa
      901 atgagggtac aaacaggata tatcagaagc gtcaaagccc tgccggggta caagctcgaa
      961 gttgagatga caacggacac ccgtattgaa ttcgatttcg cctccaggct taatacaata
     1021 cgtttcggcg cgctgaaaga cgaaaaactc ttcgtcacgg cctccacgga cggaaacttc
     1081 atcacgttcg ggacgaatga tgcggtgaag atcaggattt cggcgatcga cttcatggac
     1141 cttgtcctcg tggacagaac gggcaggttc cccccggatg aaacgtagct tcttgattca
     1201 cctcgttctc gttacgactg tgcaccaagc aaaactgctc acagggaaat cttgagcact
     1261 gcgccttttt ttcttttccg gtattctgag gacgccggaa tatatgctgt tcgtcgatac
     1321 ccggtcaagc gggtaagaaa agggaggaat acgatgaaaa agacactggt aaaaacagcg
     1381 gggcttttcg tccttgcggc gctcttggcg gtacactgcg cctccagagc agaagcctgg
     1441 atcaaaataa ccctgaaaaa caacagatcg cacaacatag cggtcgcctt ctgctggagc
     1501 ggattcgatt atgcggatga tcagagaaaa ggctggtacg ttgtccaacc caacgattca
     1561 agagttatca atctggcaaa tgccacttct tcgttgacat gggaggactt cggtttctat
     1621 gcgcagggaa cactgagaaa cgggaaaaag atcagatggt caggcgacat gaaacaggtg
     1681 attatccacc ccagtaaagc gttcgacgga catcctgaag accctatcgc gggtggcaag
     1741 aaggtgggct tccgcaaact caatttgaaa aaagtgggag acgcaaatac ggacgcggta
     1801 gcaaccctga cgtttaatcc ctgacggcga cacaaggtat gcagctctgg gcagcggcgc
     1861 ttctgttcgt ccttttcgcg gcggcggcgt tctctttcct caaaggggga aggaacaaaa
     1921 gcgggatacg cttctacacg ggcattctct tcgcgttcat cggcggtgtt gcattggcgt
     1981 acatcgcggc gacagtcttc ttcgtagttt cgatagagta agcgagtacg ggaaacggca
     2041 gtcgcaggca tgccttcgca ttcatgttga atcgaaacga aatgcgaaca tattcaaaga
     2101 aagaggtggt tcacgtgttt cattgcactc aaaagcggct ggtcacgatc atggttgtct
     2161 gctcgttcct cgtcttctcc tgcggcgcag cgctcgccgc agcggacaag gggtattcga
     2221 gcgccgacct gaagaagatg agcaccttcc tttcaaactt tacggaactg ggttttatga
     2281 gtttcgacgc gaaagagttg acgaatgaaa aagaccccgc cgatatgata cgcttcggca
     2341 tatggcacaa ttacgtcaac aacttcaaga gcagaatcag tcagtgcaaa aagaaggatt
     2401 gcgagtgggg atcccttgtt attgaaggga agtacgtcgc cgagaccatc aaaaagtatt
     2461 tcgacgtcga ctataagaaa ctcgcgagcg tgacggaatc ggacccgccg tacttttatg
     2521 acggaaagct ctaccacttc gaaggcgccg acggagaggc ggtgtattac gcaagagtca
     2581 gcgaagcgga gaagaacaca aaaggacaga tcgtcatgcg cggggagatc tacaacgcgg
     2641 aggacgaaaa cgatctgctc ggctcctttg aggcgcttgc aaaaccgcac aaattcggcg
     2701 gcaaggatac ctgggcgatc ctcagcatgg aaacagagta ctacgataat gagggacagg
     2761 tttccgacga ggctgtggag ggattgtgga tcgaggttcc caacttcccc gcggaagcgg
     2821 aagtcgtcga tttccagatg aacgacgaag gcgttgcctc gtttacgcgc atcctggacg
     2881 caggcgtcgt tgcactcgtc gtggaaagac tgcctgtcac gaataccgaa ggcgaacagg
     2941 tgacgccaca aacgctcggg aagcttgtcg ctgagtttga aggaatcgag gaagagaaaa
     3001 tagagattac cccctctctt gacgattttg caaagctgta ctcctacccc gtcgccgggg
     3061 ctgagttcac gactggagaa aacgaggata cccgcaaggt cattgatctc ttccttttca
     3121 ctgattcttg gctgttccga atccacgtgt cggtaacagc agactctttc gaggagtacg
     3181 aagatcaggt aaaagagtgg ttgtcgagcc tgaaattcag ggaatagctt agtaaaaaat
     3241 cactatacgc atgaggggta ggctccagca ccgaaaaccc ctcggatgaa gagcggggag
     3301 aaggacgtac tcggtctgcg cgattgctga aacccattgt tttgggtgaa cgcaacagcc
     3361 cggcacagaa tgtcttctct ccgtttttct atagaggatc gcgtgtattt cgtattcgtc
     3421 tctttcgcat accctcttca ccgcgattcg aggtataatt caaaaagcaa cgacatgcac
     3481 ggcgcactac catatagacc agagaaaaag gagatgatac atatgagaaa gttttttgtg
     3541 gcgcttctcg cgattgtctt gctcttcggc ggagcgtacg gtgcttgggc ggcagatgat
     3601 ttcaccattc tctcgttttc tccgggagga atggtcaaag gcaggatgcc ggtgaagatc
     3661 gccttctcgc agccagtcgt ggagaagagt ctcgtcggca agaccgcgcc gcagcagcat
     3721 ataccggttg agttttcacc gtccatacgc ggagcgggca aatggacgga tcagaagacg
     3781 ttcgtcttca ctcccgtggc gaatctccag caggcgacgc gataccgtgc gaccgccaag
     3841 gagtcgctgc aggacgcgaa ggggcggcgg ctctccggaa agcagagctt cgaattcttc
     3901 accgaaccgc tgaagctcct gagggcgcaa cagatcgact tcaccgagag caacaatgtc
     3961 gtgatcgaac tgaagttcaa cctccccgtc tcgccgttca ggctgcgcgg ttttctctcg
     4021 ctgctgaacg aacagcggca gcagctggga tactcccccg ccggacaggc gccttcatca
     4081 acagtccgcc tgacaacaaa ctccttcgaa ggggggaaaa tcgcgctcgt cctctctcca
     4141 gggctgacaa gcgacgccgg tcctctggga atagaacagg aaatccggca cgagatcgcg
     4201 gtcaccagaa agctggagat ccgcagcgtc tacgcggaca cacggtatcc cgcgacaagc
     4261 gtcatcaatg tgaacacctc cgtgggcgcg gatatgtcgg tcgtctccaa gttcatcgaa
     4321 atctcgcccg cgacggagat cagcatcgac ccggggtaca acggcttcac gatcctcggc
     4381 gacttcaagc ccaaacagcg agtggaattg accatcaaaa agggacttcc caccaaagag
     4441 ggcaaggcgc tcgaacagga gttcaagagg gcggtcatct tccccgacta ctacccgtcc
     4501 ctcgccttcc cggccggagg gaccttcctc tctcccgcag gcgatctgcg gattcccatt
     4561 gaatcagtga acatcgagga gatcacgctc aacctgtggc gtttgtacga gaacaacatc
     4621 ccggtctcca tgacgatgtc gaactactcc atacccagag accttgcccg gagagtcgcc
     4681 actcgcaccg cgcgccccga gggggacctc aacgaaaccg tgcggcgcgc catcgacctg
     4741 cgcgaactgg ccggagaggc gaagggcgtc ttcctgctga cggcgtccga gtcttcaggc
     4801 gaatactggg cggacactga acagctggtc gccgtgaccg acctgggcat cgtcgccagg
     4861 gtctgggcga acgggatcac cgtgtgggtg aactccatcc tcgggataga gcccgtcaaa
     4921 gacgcgacgg ttcgcgtcta ctcgaagagc aaccagcttc ttgcgagcgg ttccaccgac
     4981 gaaaacggtc tgtggacgaa acagggcgcc gaaccttggg atgatcagct tcttccggcc
     5041 atcgtgacgg tcgaaaaggg agacgacatc tcattcctga agtttgagag caatcttttg
     5101 gcggatacgg gcttcgatat cgacggacgc ccctggaccg acaagtacga cgcgctgctc
     5161 ttcgcgccga ggggcgtctt ccgcccgggc gagaacgtgg acttctccgc gctcgtgcgg
     5221 acaaaagacc tgctgccccc ggagccgttc cccgtggtgt acgtcgtgcg ttcgtctctt
     5281 gggcgcgaag tcgcgcgcgg aaccgcgctg ctctcttccg aagggatagc ctccttctcc
     5341 gcccgtcttg cgcccgcctc ccccacaggc acgtacagcg cggtgatagc ccttcccgga
     5401 ggagaaaacg cgcctcttgg aagaaccgag tttttcgtgg aggacttcgt cgctccccgc
     5461 ctcgaagtga aggccgtccc cgacgcctcc ttcctgaccg ctggggatac gctgaccatc
     5521 gacgtctcct cgtcgtatct cttcggcgct cctgcgtccg gactcgtctt cgagggtgag
     5581 gtgcgcgccg tggcagcgcc gttccgcccc gaagggtgga acgcgttcac cttcggcgat
     5641 atggagcgca cgttcgagag catgacggac ttcgtcgggt ccggcacact cgacgagacg
     5701 gggaagggaa cgctctcctt ctccggacct gaaggctggc ttcccccggc ggctatcgat
     5761 ctctcattct tcgtcagggt gatggaggaa agcggacgat gggtcccgac ctccgtaagg
     5821 ctccccttcc acccctaccc cgtctatttg ggtatcgaga cgcccaaagg ggacatcgtt
     5881 cccggtaagg aaatcgcagt ccgagttgcg gcagtaacgc cggagggcaa agcatcggat
     5941 ctcgaaatcg cagttgccga ggttttcctt gtccagcgcc actataacct cgtccgtacg
     6001 ggcaaccaga cgaggatgca gatgcagcgg gaacttgtcc cgcacgctca agggggcgtg
     6061 gaattcgcgg acggtatcgg ctcgttctcc ttcacaccga agatcgacgg cgagtacgtc
     6121 gtcaaaatct ccgacgaaac gtccaacagc atggcgtcgt cgacgctcta cgcttggact
     6181 ccctacggcg gcggcgcaag cggctccacg ctgctcgacc gcgttgtggt gaccgcggac
     6241 aagaagagct acgaaccggg agaaaaggcg acagtcacgc tccgaagccc cttcgagggg
     6301 aacctcctgg ttaccgtcga aacaaacagg gaacttttcc gaaaagttgt ctctctgaag
     6361 aaaggcgagg tgaatcttca ggttcccgtc accgacgaca tgattccgaa cgcctatata
     6421 acggcttccg tgatccggcc cgtcgtcgag ggtgaagagt ggggaaacca ccgggcgatc
     6481 ggcacgctct ccatcccggt ggagcgtaag gaataccatc tcgacatcgc tctgctgagc
     6541 cccgaacgca cgctcccgga cgaaccgctg aaagtgaacg gcacgctgac cgacggttcg
     6601 ggagcgcccc gcaagggcga ggtggtcctc ttccttgtag acgagggaat cctctcgctc
     6661 acagggtaca ggacgccgga cccgtgggcc ttcttcatgg cgaaacgcgc gcagggagtt
     6721 tcggtctacg atctgtacga ccagctgctc ccgttggaat caagagagac gccgctcctc
     6781 aaggcgggcg gcggcgcagg cgaagaggcc atggcggcgc ttcgcgcggg aatgtccccg
     6841 gtctccgcac ggacgttcag gatgctctcg ctcttccttg ggagcgtccc gaccgatgaa
     6901 aacggcgctt tcgaggcgac gttcgacgtg cccgagttca gcggcaaagg acggctcatg
     6961 gctgtcgcct ccgctggaaa atcgttcggc aagggagaga agttcgtcgc gatagcgcgc
     7021 gacatcacca cggaactttc gttgccgcgc gccgtctccc ccggagacac gttccacgct
     7081 ccgctgaaga tattctcctc agcgtccggc gagaagcagg tctcggtgaa ggtggggatc
     7141 gacggtcccc ttgcgctcac aggaaagaaa gagttcgttg caaaccttgc cgccggaggc
     7201 gaagcgcttt tcagcgtgcc gttcaaggcc ggcgacgaag cgggaacgac ctacctgacg
     7261 gtcgaaacgc agtgggaaga cggctctttc acacagaatc tcgatctgcc ggtgcgtccc
     7321 gccttcccga gaatcgcgct ctcgggttcg ggcgtggtgc gcggcggagg acagggatcg
     7381 atagagattc caagggactg gttccccgga actgaggagg ggcggcttct gctctccgat
     7441 cttccgatgc tcgatctcgc gggcgcggcc tccttccttc tgcagtatcc gtacgggtgc
     7501 cttgaacaga cggtttcggg cgcatggccg ctcctcgtcc tcggcgatat cgtacgcgac
     7561 gtggatcctc tccttgtgaa cgaggaggag caaaaatcgg cgctcgcgct ccgaatccgt
     7621 cagatttccg cgatgcagct ctactcgggc ggtttcgcca tgtggccggg gagctcgatt
     7681 ccctcgatgt ggggttctct gtacgccgcg cacttccttg tggaggcgaa gaagggcggc
     7741 gctcccgttc ccgacgacct gctcgacagc gccctcgcca acgtccggag catgctgcct
     7801 ctggcccctg cgaacgagag cgacgttgcg taccgggaga acatgaccct gaaagcgtat
     7861 gcggcgtata tcctcgcttt gcgcggcgaa ccgcccctcg ggtggatggc gcacctgaac
     7921 gaacatagcg atgcgctccg cgagtccgga aagatcttcc ttgccggcgc gtacgcactg
     7981 gcttccaaaa catcggaacc tctccgcaaa ctcgggacag gcgccccttc gctccggggc
     8041 ggcgagtcga cgaccttcga gtcagcgtcg aggaacaacg cgctgaaact cctcatgtgg
     8101 agcgaggtcg atcctctctc ggcgacagcc tccgatctgg ccctcaaact gatcgaagag
     8161 gcccgcagca gcacatggag aacaacgcag gacaacggca tggccgtgct cgcgctcggc
     8221 cgttggatgg agaagacgcg gggggcgcgc aaaccgttca ccgcgcttgt gaaggatacc
     8281 tccggcgcga cggtcgcttc gttcaccgac ggaaagcggg tttctctgag cctgaaggag
     8341 gttccgcagg gaccgctgac gctctcgctc gacggcgagg gaaccgccta cttcgcgtgg
     8401 acgtcggccg gcgtaccgat gaaggcgccg aagccatcga gcaacggaat cgccctcaaa
     8461 cgcacatgga gcgggcgtga cgggaagccg ctcgcggaag gcgcgcctct ttccaggggc
     8521 gaccggatca cggcgacgct cacgatgacc ccctcaacag cgttgagcga tctggtggtc
     8581 gttgatatgc tgccgggcgg tatggagatc gagaacc
//
LOCUS       DAOPKU010000050         8600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8691_length_8600_cov_4.639321, whole genome shotgun sequence.
ACCESSION   DAOPKU010000050 DAOPKU010000000
VERSION     DAOPKU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8691_length_8600_cov_4.639321"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..957)
                     /gene="cobM"
                     /locus_tag="PK849_02600"
     CDS             complement(124..957)
                     /gene="cobM"
                     /locus_tag="PK849_02600"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HPE65044.1"
                     /translation="MANTEYPVLFVGAGPGDPELITLKGKRALEEADLVLYAGSLVNE
                     ALLDYCSPGCVRADSSPLSLEEQMALVAAKVREGGRVVRLHTGDPSLFGAVAEQKERL
                     ESLGIEVRFIPGVSSFQAAAAALGIQYTVPGGSQTVVCTRRSGRTPVPPRENLRLLAE
                     HGATMVVFLSADQAEEVSADLQAGGFSAETPAACVYRASWEDEKTIVSTLGALPSLMA
                     EHGITRHALIVVGECVASHGARSLLYSPHFAHGARDAAPEQSDEERTLASSEGFCEAP
                     L"
     gene            complement(1008..2291)
                     /gene="cbiE"
                     /locus_tag="PK849_02605"
     CDS             complement(1008..2291)
                     /gene="cbiE"
                     /locus_tag="PK849_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HPE65045.1"
                     /translation="MGRFTVVGLGPGDDSCLLPCARRAIEDAEILAGARRHLAPYEKS
                     GKTLLFLEKGVDELLDAVDKHRKLGRVALLLSGDPCLFSLLGRIAARFPSGDYEVVPG
                     LSSFQLLFARLASLPGEPGAPSPSWSDVRLESIHGRPIEGVASMLHEHRRTLLFLDSK
                     NSGPAVAAFLRDRGFPDRRVILAERVGYENERISPTTLFNLANERDAEGLALLLVEPG
                     PIPPLSKGVLPDSWFVRHERTPLSKEITRALTVSFLLPLDGLSVLEVGTGSGGITVEL
                     ARRIAGGNVVSVERSPDALAVAEENLRRAGVFDRVRLLRGSAPEVFAELNDRSGAPLR
                     FHRAVIGGHGGASGEIATAAWNRLLPGGRLLATANMPSSADAIYRALKSLGATPSLTH
                     VNASSARESAGQGAEASWMLLASNPVFLIYADRNA"
     gene            complement(2264..4180)
                     /gene="cbiD"
                     /locus_tag="PK849_02610"
     CDS             complement(2264..4180)
                     /gene="cbiD"
                     /locus_tag="PK849_02610"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HPE65046.1"
                     /translation="MDEQRTRRHGAEFKTENGEEPVRKRDRDLSPSEIEEKSFRILEG
                     LLGDFQAPAPEREVMKRVAHATTDVEWAKTFRFSPNAVQAGIEAVRRGAPVVTDVEMV
                     SAGVRRSAAEARRFRNAQDGNETLCFLNDPDVAEEAKRNGTTRARLAIRKAIPYLDGA
                     IVAIGNAPTALFEVCDLIRRGECRPALVVGVPIGFVGAAESHDELASLDCEWITASGP
                     KGGSPVAAAVVNAIIRLALKDAPPAPEKGGRRGFTTGSCAAAAAKAAATLLVTGEAPC
                     SAELRLPSGGAVLRIPVEACELSPSGARCSVRKDAGDDPDVTDGMLVVAEVTLSEEPG
                     ITISGGRGIGRVTRKGLPLPPGEWAINPTPRTMIRDALETLLPPEKGLSVTISAPEGE
                     ERAAKTWNPRLGIEGGISILGTTGIVEPKSTAAYLASIDLYISAALAFDSPRPGTIFL
                     VPGYVGEKTLEERYAVPRELIVRTGDHFGHALKKSVELGARRLHLFGHVGKWAKVAAG
                     LFNTHCDYGDARLETLAACAGACGATPEQIRELLALPLAEEAVPLIERWGLSKTFDVL
                     AERMHRRCALYAVSRASLVEPQRKSAETRETPPGKTVAFGVAVLSLKGDILGSFPSLS
                     LKEGSSWEDLPSSV"
     regulatory      complement(4382..4497)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4672..5343
                     /locus_tag="PK849_02615"
     CDS             4672..5343
                     /locus_tag="PK849_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE65047.1"
                     /translation="MSLKETYKTKSGFVYELLRERIVAGEWPQGTVIVVASVARELGV
                     SAIPVREAMKQLQAEGLVELEPHRGARVTVFDPKKIVEVMSIRGALEGYAARVALPFI
                     DDAALADLRRKVDDMGTFTVAGDTARLVAANKEFHRKLYSMGPFPMLNDMIFKLWDGG
                     AFSSIVFALRPDRIRTVYEEHLAILAAIERRDAEELERLVRAHKSGFAEALCEISARK
                     RLETL"
     gene            5386..6153
                     /locus_tag="PK849_02620"
     CDS             5386..6153
                     /locus_tag="PK849_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPE65048.1"
                     /translation="MKVFRYSVCAALIVCLCLALAVPVLADPAEMLLAKITGTNVNVR
                     AEPSRTGRLTGRFTGGEHAVVLEERSGADPFPWSRVIAFASGGTLVEGWVYGQYVEPL
                     PQDDWSNSAEGDPGERFSRFFDKTLERIGDTPEEAAARFGAPAAREDTRLPGRHDPAY
                     MVDYFRLEYPAFELLYFKTKDSYGLIGVKIVEGEAVIGEGIRLGADAASVVRELGVPL
                     YQKGSAFAWTDEPGYGVLTLTFAEGRVLNIDLDVELD"
     gene            6228..6863
                     /locus_tag="PK849_02625"
     CDS             6228..6863
                     /locus_tag="PK849_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65049.1"
                     /translation="MRVQTSDVLKFLFLYVLFAASVSSPALCFDDSILPPLPEDELAQ
                     PHPQEDLPESGDVSGLDPSEVKTTGGVLAGVSSDIFLPPLDADPEARARAVAIWNGAA
                     ELQRGKKYEEALEKYREGLAVCEDPVVREHIAKLEAFLELQRKKAEASSARARAVALW
                     NEAAELQLAKKYEEALEKYREGLAIASDPVVEEHVKKLREFIEKRKARGKQ"
     gene            complement(7039..8067)
                     /locus_tag="PK849_02630"
     CDS             complement(7039..8067)
                     /locus_tag="PK849_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE65050.1"
                     /translation="MAANRTIRTCAFLLFVLLVGGSVLAPLPSFSFVPPPLPGDEEDI
                     EPPPPPIFDETPPAQEQPAPTKVRPTPTPEPASQQPAPKPKPKPKPKPAPKPKEPSYT
                     ADQLFRMGMDAYKGEGGAQQSFQEALKWWTRAASAGHVVAQYNLGIMHQNGEGTSKDY
                     KKARDYFLKAAGKGYANAAEQLGHLYFGDFLGAPEYGEAGKWYEQASAKGNLPFAEFR
                     LGLIHYKGLGRPKDSMKAFYWFDRAARNGYIDAMVNLGVLYEKGDGAVFSPEKAIRWY
                     TEAAEKGSFLGMAYLGEAYYQGRGVRKDEKKAVGLLEQAASSGLPYAQRLLYRIDRIK
                     YMRYRDVQ"
BASE COUNT         1569 a         2590 c         2714 g         1727 t
ORIGIN      
        1 ggttccacca cgaggcgacc gtcgaggcaa gggttccgga ctcgacgcgg acgacggtcc
       61 ccgcctcttc aagacggcgg gctgtcgctt ctcccttcga cgagaagcag aaaaacgccg
      121 tcttcatagc ggagcctcgc agaagccttc ggacgaagcg agcgttcgtt cttcgtccga
      181 ctgttcggga gcggcgtccc gcgcgccgtg ggcgaagtgc ggcgagtaga gcaggcttcg
      241 tgcgccgtgc gacgcgacgc actctccgac gacgatcagc gcgtggcggg tgatgccgtg
      301 ctccgccatc agcgaaggga gcgcgccgag cgtggagacg atcgtcttct catcctccca
      361 ggaggcgcgg tagacgcagg cggcgggtgt ttcggcggag aatccccccg cttggaggtc
      421 tgcggagacc tcctccgcct ggtccgcgct gagaaagacc accatcgtcg cgccgtgctc
      481 cgccagcagg cggagattct cgcggggagg cacgggcgtc cgccccgatc tgcgggtgca
      541 gacgaccgtc tggctccctc cggggacggt gtactgaatg cccagtgcgg cggcggcggc
      601 ctgaaagctg ctcactccgg ggatgaaccg gacctcgatg ccaagcgatt ccagccgctc
      661 cttctgctcg gccacggccc cgaagaggct cgggtcgccg gtgtgcaggc gcacgacgcg
      721 cccgccttcc ctgacttttg ccgcaaccag cgccatctgc tcttcgagcg aaagcggcga
      781 cgaatcggcc ctaacgcagc cgggggagca gtagtcgagg agcgcttcgt tgacgaggct
      841 ccccgcgtag agcaccaggt ccgcctcttc gagcgcgcgc ttccctttga gtgtgatcag
      901 ttccgggtca cccggacccg cgccgacgaa gagcacgggg tattctgtat tcgccattgg
      961 ttttccctca ctttgtgtgt tccagtgcgc gttctcccgt atacccgcta cgcgttcctg
     1021 tcggcgtaga tcaaaaagac cgggttcgag gcaagcagca tccacgacgc ctccgctccc
     1081 tgtccggcgc tctcacgcgc cgacgaggcg ttcacatgcg tcagcgacgg cgtcgcgccc
     1141 aggctcttca gcgcgcggta gatcgcgtcg gccgaggagg gcatgttcgc cgtcgccagc
     1201 agcctccctc cgggaagaag acgattccac gcggctgtgg cgatctcgcc tgaggcgcct
     1261 ccgtgaccgc cgattacggc acggtggaaa cggagcggcg cgcccgagcg gtcgttgagc
     1321 tctgcaaaca cctcgggagc ggagccgcgg agcaggcgga ccctgtcgaa aaccccggcg
     1381 cggcgcagat tctcctccgc gacggcgagg gcgtccggcg agcgttccac cgaaacgacg
     1441 ttgcctcccg ctatccggcg tgcgagttcc acggtgatcc cccccgaacc cgttcccact
     1501 tcgagcacgg agagcccgtc gagagggagc agaaaagaga cggtaagggc ccgcgtgatc
     1561 tccttcgaga ggggggtgcg ttcgtgccgg acaaaccagg agtcaggaag gacccctttg
     1621 gagagcggcg ggatcggtcc cggctcgacc aggagcagcg ccagtccctc cgcgtcccgt
     1681 tcgttcgcga ggttgaagag cgtggtcggg gagatgcgtt cgttctcgta ccccaccctc
     1741 tcggcaagga tgacgcgccg gtcgggaaag cccctgtccc gcagaaacgc cgccacggca
     1801 gggccggagt tcttcgagtc gaggaagagc agcgttctcc tgtgctcatg gagcatcgag
     1861 gcgacccctt cgatggggcg tccgtggatg ctttcaagac gcacatcgct ccacgagggg
     1921 ctcggagcgc ccggttcccc cggaagcgac gcgagtcggg cgaaaaggag ctggaagctg
     1981 ctcaggccgg gaacgacctc gtagtcgccg gagggaaaac gggccgcgat ccgtccgagg
     2041 aggctgaaaa ggcagggatc gcccgagagc agcagcgcga cccgaccgag tttccggtgt
     2101 ttgtccaccg cgtcgaggag ctcgtcaacg cccttctcca gaaagagaag cgtcttgccg
     2161 cttttctcgt acggcgcaag atgccgccgt gctccggcga ggatctccgc gtcctcgatc
     2221 gcgcgccggg cgcaggggag caggcaggag tcgtctccgg ggcctagacc gacgacggta
     2281 aatcttccca tgacgacccc tccttcagcg aaagagaggg aaagctcccc agaatatccc
     2341 ccttgagcga gagcaccgcg acgccgaagg cgacggtctt cccagggggt gtttctcgcg
     2401 tctccgcgct tttccgctgc ggctcgacca gagaggcgcg ggagacggcg tacagggcgc
     2461 accggcggtg catccgctcc gccagcacgt cgaaggtctt cgaaagcccc cagcgctcga
     2521 tcagcggaac cgcctcctcc gccagcggca gtgcgagcag ttcgcggatc tgttcgggcg
     2581 ttgcgccgca agcgcccgcg cacgccgcaa gcgtttcgag cctcgcgtcg ccgtagtcgc
     2641 agtgcgtgtt gaacagcccg gcggcgacct tcgcccactt accgacgtgc ccgaagaggt
     2701 ggaggcggcg cgcgcccagc tcgacgctct tcttcagcgc gtgcccgaag tggtcccccg
     2761 tacggacgat cagctcgcgt ggaacggcgt aacgttcttc aagcgtcttc tccccgacgt
     2821 aaccggggac gaggaagatg gtccccggcc ggggagagtc gaaggcgagc gccgccgaga
     2881 tgtacaggtc gatggaggcg aggtaggcgg ccgtagactt cggctcgaca atgccagttg
     2941 tgccgaggat ggagatcccc ccttcgatgc cgaggcgcgg gttccacgtc ttcgcggccc
     3001 gctcctcccc ctcgggggcg gagatcgtca cggacagccc cttttccggg ggaagcagcg
     3061 tttcgagcgc gtcgcggatc atcgtccggg gagtcgggtt gatcgcccac tctccaggag
     3121 ggagggggag ccccttccgt gtgacgcgcc cgatgccccg gcctccggag atcgtgattc
     3181 cgggctcttc ggagagcgtg acctccgcga cgacgagcat gccgtcggtg acgtccgggt
     3241 cgtccccagc gtccttgcgg acagaacagc gggcccccga tggggagagc tcgcacgctt
     3301 ccacggggat gcgcagcacg gctccgccgg aaggaagacg gagctccgcg gagcaagggg
     3361 cctccccggt gacgagcagc gttgccgcag ccttcgccgc cgccgccgcg cagcttccgg
     3421 tcgtaaaacc tcgcctgccg cccttttctg gagccggggg agcgtccttg agcgcgaggc
     3481 ggataatagc gttcaccacg gccgcggcca cggggcttcc gcccttcgga ccggaggcgg
     3541 tgatccattc gcagtcgagc gacgcgagct cgtcgtgcga ctccgccgcg ccgacgaagc
     3601 cgatggggac gccgacgacg agcgccgggc ggcactcccc ccgccggatc agatcgcaga
     3661 cctcgaagag ggctgtcggc gcgttgccga tggccacgat cgcgccgtcg agatacggaa
     3721 tagccttccg tatcgccagg cgcgcgcggg tcgttccgtt ccgtttcgcc tcctcggcga
     3781 cgtcgggatc gttcaggaag cagagcgttt catttccgtc ttgggcgttc cggaatcgcc
     3841 gcgcttccgc tgcggaacgg cgaacgcccg cgctcaccat ctcgacgtcg gtcacgacgg
     3901 gagcgccgcg ccgtacggcc tcgatgcccg cctgcactgc gttcggcgag aagcggaacg
     3961 tcttcgccca ttcgacgtcg gtcgtggcgt gggcgactct cttcatcacc tcgcgctccg
     4021 gagcgggagc ttgaaagtcg cccagcagtc cctcaagaat ccgaaagctc ttttcttcta
     4081 tttccgaggg ggagagatcg cggtcgcgct tccggacagg ttcttccccg ttttctgttt
     4141 tgaattccgc gccgtgccgc cgcgtcctct gttcgtccat ggaatctcct ccattcatac
     4201 ggatacgcgg agcgcgcacc gctgaagaca aaaataaggc gaacccccgt gtgaggttcg
     4261 ccggcgccgg tcatatatat gcgacgagtg caaaatccgg aaaaacctgt ccgtttgccg
     4321 caaaaaatac atgaaaagcc gacccgaacc ctcgcaggtg tcgcacagat gcgcttcttc
     4381 agggtcttct ggcttccgtc atcctcgttc accccttccc ggcaaaaacc ggtggtcgat
     4441 gaactcgtcg ggtacacagc ggcgggaccg cgccggaatt tcaccgggct tccccttgaa
     4501 gacgagcgcg ttcatatagt attcgatgaa catctccatc ctaatcttcc ggtgtcgacc
     4561 tgtcaatcgg atttcgtgga acgggtgcat ttcttcgttt gactaggtat aatatgcatc
     4621 atatattgtg cttttaaaaa gggcttgatt tttcaaaatc aggaggtttc catgtcgctc
     4681 aaagagacgt ataagacaaa gtcggggttc gtatacgaac tgctgcggga gagaattgtc
     4741 gccggagagt ggccccaggg aacggtgatc gtcgtcgctt ccgtcgccag agagctcgga
     4801 gtctcggcga ttccggtgcg tgaggcgatg aagcagcttc aggccgaagg acttgtcgaa
     4861 cttgagccgc accggggggc gagagttact gtattcgatc cgaagaagat cgtcgaggtc
     4921 atgtcgattc gcggcgcgct cgaagggtat gccgcccggg tcgcccttcc attcatcgac
     4981 gacgcggcgc ttgcggattt gcgccgcaag gtggatgata tgggaacgtt tacggtcgcc
     5041 ggcgatacgg ctcgtctggt tgcggcgaac aaggagttcc atcggaagct ctattccatg
     5101 ggccccttcc cgatgctgaa cgacatgatc ttcaaactct gggacggggg cgccttttcg
     5161 agcattgtct tcgctctcag acccgaccgt atcaggacag tgtacgaaga gcaccttgca
     5221 atccttgccg cgatagagcg gcgcgacgca gaggaactgg aacgcctggt acgggcgcac
     5281 aagagcggtt ttgccgaggc gctttgtgag atttctgcga gaaagcggct cgagactttg
     5341 tagcggcagt atccggtact ttcatcccga aaggagttgg aattggtgaa ggtttttcga
     5401 tattcagtat gtgcggcgtt gattgtgtgc ctgtgtctgg cgcttgccgt gcccgtcttg
     5461 gccgacccgg cggagatgct cctggcgaaa attaccggga cgaatgtgaa tgtgcgagcc
     5521 gaaccatccc ggacaggccg tctcacgggg cgttttacag ggggcgagca tgcggttgtc
     5581 ctcgaagaac gctccggcgc ggaccccttc ccgtggtcgc gggtcatcgc attcgcttcc
     5641 ggagggacgc tcgttgaggg ctgggtctac ggccagtacg ttgaaccgct gccccaggac
     5701 gactggagca atagcgccga gggagatccc ggcgaacgct tttcacgttt ttttgataaa
     5761 accctggaac gcatcgggga tactccggag gaggcggccg ccaggttcgg cgctcctgct
     5821 gcgcgtgagg ataccaggct tcccggccgg cacgaccccg cctacatggt ggactatttc
     5881 cgccttgagt acccggcctt cgaacttctc tatttcaaga cgaaagacag ttacggtctg
     5941 ataggagtga agatcgtcga gggcgaggcc gtgatcggcg agggcattcg tctcggcgcc
     6001 gacgccgctt ccgtggtccg ggaactcggc gtcccgttgt atcagaaagg ctctgccttc
     6061 gcatggaccg acgagcccgg gtacggcgtc ctcaccctta ccttcgcgga ggggcgcgtt
     6121 ctcaatatcg acctggacgt cgaactggat taggaggcat ggcggggcgc tttttctcgt
     6181 gagagcgcgt atgcgtcgat actttttttc tggaggtgtt ttttgccatg agagtacaga
     6241 cctccgatgt tctgaagttt ttgttcctgt acgtgctgtt tgcggcgagc gtatcttctc
     6301 ctgcgctctg cttcgacgac agtattttac cgccgcttcc cgaggatgaa ctcgctcagc
     6361 ctcatcctca ggaagacctc cctgagagcg gcgatgtttc cggactcgac ccttccgagg
     6421 tgaagacgac gggaggcgtt ctcgcaggag tatcgtcgga catcttttta cccccgctgg
     6481 acgcggaccc ggaagcccgt gctcgcgccg ttgcaatttg gaacggcgct gcggagttgc
     6541 agcgtgggaa aaagtacgaa gaggcgctgg agaagtaccg cgaggggctt gcagtgtgcg
     6601 aggatcccgt tgtccgcgag cacatagcaa agctggaggc atttcttgag cttcagcgga
     6661 aaaaggcgga agcctcctcg gcacgtgcgc gcgccgttgc gttgtggaac gaagccgctg
     6721 aattgcagct cgcaaagaag tacgaagagg cgctggagaa gtaccgcgag ggactcgcca
     6781 tagcatccga ccctgtcgtc gaggagcatg tcaagaagct ccgggagttc atcgagaagc
     6841 ggaaggcccg cggaaagcag taaacaagga gcgccccgat gaaacaattt tatcgaatgc
     6901 tctccgaaac gccgctccgg cggaagtgtt ccgtgtgcgg aagaagagac ctcatcccga
     6961 tgaaggggat gaggtctcta taactatggt cgggcgccga aagcctgccg cacttccggg
     7021 gacagcgtgt atgtcgggct attgaacgtc gcggtaccgc atgtacttga tcctgtcgat
     7081 ccggtagagc agtctctgcg cgtagggcag ccccgacgag gccgcctgtt cgaggagccc
     7141 caccgctttc ttttcgtctt ttctgacgcc cctgccctgg tagtaggcct ctccgagata
     7201 cgccatacca aggaaagacc ccttctccgc cgcttccgtg taccagcgga tcgctttctc
     7261 cgggctgaag acggcgccgt cgcctttctc gtagagcacc cctagattga ccatggcgtc
     7321 gatgtacccg ttccgggcag ctctgtcgaa ccagtagaag gccttcatcg agtccttagg
     7381 gcgtccaagc cccttgtagt gaatcagacc caggcggaac tccgcaaagg gtaaatttcc
     7441 ttttgcggag gcctgttcat accacttccc ggcttcgccg tactccggcg cgccgagaaa
     7501 atctccgaaa tagaggtggc cgagctgttc ggccgcgttc gcataccctt tccccgccgc
     7561 cttgaggaaa tagtcccgcg ccttcttgta gtcctttgaa gttccctcgc cgttctggtg
     7621 catgatgccg aggttgtact gcgcgacgac atgacccgcc gaggccgcgc gcgtccacca
     7681 cttcaatgct tcctggaagc tctgctgcgc ccctccctcg cccttgtagg cgtccattcc
     7741 catcctgaac agctggtcgg cggtatagga cggctccttt ggtttcggcg caggcttggg
     7801 tttcggcttc ggtttgggct tcggcgccgg ctgttgagag gccggttcgg gcgtcggagt
     7861 cgggcgcacc ttcgtcgggg caggctgctc ctgcgcgggc ggcgtttcgt cgaaaatagg
     7921 cggaggcggc ggttcgatgt cctcttcgtc gccgggaagg ggagggggaa caaaactaaa
     7981 agaaggaagc ggcgcgagga ccgatcctcc aaccagaaga acgaacaaaa ggaatgcgca
     8041 tgttcgaatc gtcctgtttg cagccatttg cacaaccccc tttaggaaat ctggcggaca
     8101 ggacaggcga acctctctgc ccgctgaacg aaaacgccct tctgcctgtt tggaacaaac
     8161 ccggaaggcg catcaatctg accataatat taacagattc cggcgggtgg cgcccttttt
     8221 ctgtctgaga atttgtttct ttttatctgt tggattatgt atgcaataat atgactatgc
     8281 tatattatct gggctgattt attccatttg gctcttagat atgtatattt tgtctgaaag
     8341 ggaaagtcaa ttgttcttta tgagtctaaa ttttattttt ttttgggcga gctttcctga
     8401 aagaagtgtt gtatttattg cgcttctcca agtcggagtg atataatcgc gtatgcatgc
     8461 aatgctattt ggattgtact gtaattacat aaatgagtaa aatgatagtt ggtatgctca
     8521 aaacagatgt ttcagtgtta gcggaatgct ttttcgagtt tttaaagtat cgtgcattgt
     8581 cgtttttgac gaaaatatga
//
LOCUS       DAOPKU010000051         8555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8743_length_8555_cov_5.152588, whole genome shotgun sequence.
ACCESSION   DAOPKU010000051 DAOPKU010000000
VERSION     DAOPKU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8743_length_8555_cov_5.152588"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..653
                     /locus_tag="PK849_02635"
     CDS             123..653
                     /locus_tag="PK849_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPE65051.1"
                     /translation="MKRRLGLLSDTHGHEDAWMKASLLWGNSLDAVLHAGDVLSSDVD
                     TGLAGMIRTAPFPVIISRGNCDYPEDEKLLHRPILSPYAFVWWNGKSILMAHGNAFEQ
                     FRSLALRSRVDLAVYGHTHVASVVREEGTLFVNPGSASLPRGRDPASAALIDEDGVVI
                     FTLDGAELHREQWIYS"
     gene            691..1215
                     /locus_tag="PK849_02640"
     CDS             691..1215
                     /locus_tag="PK849_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPE65052.1"
                     /translation="MNPTLATKETQDLKAREVESRGTERITLVFALYEEDFGLDVTYV
                     REIVRVPPFITRVPNSPDYIRGVINLRGSIVPVFDMELKIGMMQKELTEEARIVVVSW
                     NEILFGIIVDSVREVCTIYDSQIESAAKLNTTMDKKYLLGVAKHEDGRLIVLLDLAAL
                     FDIDQILDEEAFNG"
     gene            1227..2099
                     /locus_tag="PK849_02645"
     CDS             1227..2099
                     /locus_tag="PK849_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPE65053.1"
                     /translation="MIRIDLHLHSTFSDGSMTPEALVAHGRSKGVTVMSLTDHDTVDG
                     LPSFMAACARWNMAGIPGIELSADHARTVHILGYRLDLRRAGSASSASAQGNTPSMGM
                     KKMLEEVRRGRDERNAAMCENLRRMGLDITLDEVALEAGGDVVARPHMARVLVRKGYA
                     QDIVSAFARYLNTSSPAYVERYRLSPEDCIRMIADAGGLAVLAHPAQTTGDLSELREI
                     VRMLKDAGLWGIECVSSRASAEQIFRYLSLAAEFSLFPTAGNDFHGANRPSAMMGVAV
                     AEDFLPWARLGVSL"
     gene            2279..3310
                     /gene="ltaE"
                     /locus_tag="PK849_02650"
     CDS             2279..3310
                     /gene="ltaE"
                     /locus_tag="PK849_02650"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HPE65054.1"
                     /translation="MFPVDLKSDTVTKPSEEMRKAMYAAEVGDDVYGEDPTVNRLEET
                     AAALTGMEAALFLPSGTMGNLVAVLTHCGRGDGAILGADCHILNYEGGGLAALGSVVP
                     LVADDSSGVISPEEVRTHCRPSNVHFAPARLLCLENTHNRRGGLALSPEAVRSSAQEA
                     HRLGLSVHIDGARIFNASTAWSCSVKKYAESVDSLQFCISKGLGAPVGSLLCGRRSFV
                     DAARHWRKRLGGGLRQSGILAAAGLYALEHNIERLREDHENADLLAHLLSEGTVVKVE
                     HNEKPTNMVYCAVPDGACADLHLRCADRGVLFNGVAENRFRLVTHLDVSREQVTRAAA
                     IILEEAAAS"
     gene            3307..4668
                     /locus_tag="PK849_02655"
     CDS             3307..4668
                     /locus_tag="PK849_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPE65055.1"
                     /translation="MKKFPERTAAESAASFLIERAVKRGASAADVLYTFNSGHSLSLL
                     DGEPEKISSGFSLGIGLRSLDSAGRQGVAHVNSLDRNLLEEVVEWSFSNCMSSEPDAF
                     VALSDPIPACTEDLALTDEAVLNLSSSDRMAFCREMWETARSADPRVVSVRSASWGDG
                     AGEVLYASSTGAMMWYEGASAGCGVSVILSSGDEMEMGGYGDESRFLASLQPRATALE
                     AVRRTAMVLGGEPLPTGKYDLLLDPESSASFVEVLGELFLASNIHKNKSFLKGKLGEK
                     IASPALTLVDDGTLKGGIGAAPFDGEGIPCSRTCLLSGGVVESWLYNLKYARIDGVAS
                     TGNAARSVSGTPDVDCSNLFLLPGKWTPQDLLLQVGSGIMVTELLGLHTMNPVSGDFS
                     LGIKGVRIKDGTLSEPVAGMTVAGNLKDVLRSIDLVGNDFKFFGSIGSCSLVIRDVTA
                     AGT"
     gene            4676..6265
                     /locus_tag="PK849_02660"
     CDS             4676..6265
                     /locus_tag="PK849_02660"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPE65056.1"
                     /translation="MLLLFLFAMFLPAEAESALQLFSGNQCLGNVNSLQQGAFSLVSA
                     TDMASLLELPAVAKDDTLVVTAGRNKIQLVSGASAAWLDVELVPLAASCVQRNGQWFL
                     ESGSALKLFHSLLSRSGKNVSLRWGTEVSDGAPAPLVTPPEPTKTPSSPVRDASAVSA
                     PSSARPLLKGVRWGIDDDKIRVVLDLEGTGEPEVQRGAGSMKVFFSSGPWPVPGIPSP
                     YREVNAGAMNFGDRLMIDFASSLVVHEILSLEAPKRLVIDFRRSAASGAPVVPPVVVH
                     APRPTPLPTLSPLPPSTVETPRSPSRTGKRIVVVDAGHGGKDPGAVANKIREKDLNLS
                     VSRMIVARLKEFGIDARLTRDSDIYLTLRRRTEIANTMNADLFISIHGNALPPGKSAT
                     GMEIYLMALPTDKDAMELARFENRELAEDNGDSTKAADKRTQTLLAILGNMQQNAKIS
                     ESTGFAEYLFNGGKAGGLKMRRVAQAPFYVLRGAAMPAVLVELGFLTDRQEAKLLADK
                     SYQRKMADALAGGIREYLKKN"
     gene            6287..6976
                     /locus_tag="PK849_02665"
     CDS             6287..6976
                     /locus_tag="PK849_02665"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPE65057.1"
                     /translation="MGRKYDDFEDGFEVRRDEAPRSSRRSAANREGGRKAPLFIRLIA
                     WTGVLVFCFAAGYVGTSVALRMLNRQDILQREDVAANRGEAEKILNEAPKEIRLNARK
                     VEFTLYYPKDGTISSEKAEVLSGIMEDDIRQVIGKILSTVSGKGASDVKLLNLFRSGD
                     TLFLNFNAHFLSLLKAQGEQGSALLITSVVRTMNENFSPIVQVRFLVDGKEQKGGAPV
                     DLSVPWRLPRG"
     gene            6987..7586
                     /gene="rdgB"
                     /locus_tag="PK849_02670"
     CDS             6987..7586
                     /gene="rdgB"
                     /locus_tag="PK849_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPE65058.1"
                     /translation="MRAPGLERLLFASGNRGKYVEVIDLFAPLGVDILYGPDVVVLDV
                     EETGSSYGSNAFLKAKAYAAASGMPALADDSGMEVRALNWEPGIFSARVAPTDGERIE
                     TILGRMRGVADRYARYVAAFALYFPSGSCIVSEGVCPGTLGEAPAGDRGFGYDPVFSP
                     SGYDRTFGELPDEVKRKISHRAVAGYRLMDILSGNCVVE"
     gene            7632..7856
                     /gene="secG"
                     /locus_tag="PK849_02675"
     CDS             7632..7856
                     /gene="secG"
                     /locus_tag="PK849_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPE65059.1"
                     /translation="MKTFLGIVHILLCIALSGAILLQQRKQGGFSGIFGGGTQADMGG
                     SQWQRFTGLSKVTVILTAIFMVTSIVLVLL"
     gene            7911..>8555
                     /locus_tag="PK849_02680"
     CDS             7911..>8555
                     /locus_tag="PK849_02680"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPE65060.1"
                     /translation="MESFSDVRDFRPEGGRLYSATHEEILGGCTTDVYFVKTRDVLRK
                     SGKLSTDVTAEIFARKNGVFAGLDEALLLLKGKNLAVESLREGDPFGPKETLMRIQGS
                     YELFGTYETVLLGMLASASAWATAAKACVEAARGKPVLCFGARHVHPSIAPVMERTAV
                     RVGGCSGASCILGAKLAGIEPVGTVPHAAVLIMGDTVDLAMEYDAVMPSGDPRIV"
BASE COUNT         1686 a         2378 c         2641 g         1850 t
ORIGIN      
        1 gcaagcgatg aaaaagcccg gagcatgcta caataaatga gtatcggggc ttttgttcct
       61 ctccatttta gtacactttg ccgtattggg gaacatcccg ttgtcaggag gggaaccgga
      121 taatgaaacg tcggcttggt ttgctgtcgg acacgcatgg gcatgaagat gcctggatga
      181 aggcatcctt gctgtggggt aattctcttg acgctgtcct gcatgcaggc gatgtgctct
      241 cttcggatgt cgataccgga ttggccggga tgattcgcac cgcgccgttt cctgttataa
      301 tttcgcgggg aaactgcgat tatcccgagg atgaaaagtt gttgcaccgg cccattcttt
      361 cgccgtacgc ctttgtatgg tggaacggga aaagtatcct tatggcgcat ggaaacgctt
      421 tcgagcagtt ccggagtctt gcgttgcgaa gcagggtcga ccttgcggtg tacggacata
      481 cgcacgttgc ctccgtggtg cgcgaggaag ggaccctttt tgtcaacccc ggttccgcgt
      541 ctcttccgag ggggagggat cccgccagcg ccgcactcat cgacgaagac ggcgtcgtca
      601 tcttcacgct tgacggtgcg gagctgcaca gagaacagtg gatatactca tgatatattc
      661 gagagagatt ctaccgggag gggaaagaaa atgaatccga cgcttgcgac taaagaaaca
      721 caggatttga aagcccgtga ggtggaaagc agaggaacgg agcgtatcac gcttgtgttc
      781 gctctgtatg aggaagactt tggtcttgat gtgacgtacg tcagggagat agtccgggtt
      841 cccccgttta tcacgagagt tcccaactct cccgactata tccggggcgt tatcaacttg
      901 cggggatcca tcgttccggt cttcgacatg gagctgaaga tcggcatgat gcagaaggaa
      961 ctgactgagg aggcaaggat cgtcgtggtc tcctggaacg aaatcctctt cggcatcatt
     1021 gtggactccg tgcgggaagt atgcactatc tacgacagtc agatcgagtc cgccgccaag
     1081 ctcaacacta ccatggacaa gaagtatctc ctcggtgtgg cgaagcatga agacgggcgt
     1141 ctgatcgtgc tgctcgatct tgctgccctg ttcgacatcg accagattct cgacgaagaa
     1201 gcattcaacg ggtgagtcgg ggccgtatga tccgtatcga cctgcatctc cacagcacct
     1261 tctcggacgg ctcgatgacg ccggaagccc tcgtggccca cggcagaagt aaaggcgtga
     1321 ccgtcatgag cctgaccgat cacgatacgg tcgacgggct cccttcgttc atggcggcgt
     1381 gcgcgcgatg gaacatggcc ggtattccgg gaatagaact ctcggccgac catgcgcgta
     1441 ccgtccacat cctggggtat cgattggatc ttcgccgcgc agggagcgca tcttccgctt
     1501 ccgcgcaggg gaatacgcct tccatgggca tgaaaaaaat gcttgaggaa gtgcgtcggg
     1561 gaagggacga gcggaatgcc gcgatgtgcg agaatctgag gaggatgggg ctcgacatta
     1621 ctctggatga ggttgccctg gaggcgggcg gcgatgtcgt cgcgcgcccc cacatggcaa
     1681 gagtgcttgt gcgaaaagga tacgctcagg acattgtttc tgccttcgca cgttacctga
     1741 acacatcctc tccggcgtat gtggaacgct atcgtctttc gccggaggac tgcatccgga
     1801 tgatcgccga tgcgggaggg ctcgccgttc tcgcccaccc cgcgcagact accggcgatc
     1861 tttcggaact ccgggagatc gtccggatgc tcaaggacgc cggattgtgg ggaattgaat
     1921 gcgtctcctc gcgggcttcc gcggagcaga tcttccgcta cctttcactt gcagcggaat
     1981 tttctctctt cccgacggcg ggcaacgatt tccacggcgc caaccgtccg tccgccatga
     2041 tgggcgtggc ggttgcggag gatttcctcc cttgggcgag gctgggcgtt tctctgtaac
     2101 gtcctgcttg caggggcttc gatgaagctg cgggcgaatt tcggcgggaa ctaccctccg
     2161 gaattcgctc ttttgatggt attatatcag ccggagcttt tgtataatgt tgatttgaga
     2221 atgtaattgc cacacggcat attcgtgaac gctctggttt aaaggaggaa ttggacacat
     2281 gtttccagtt gatcttaaaa gcgatactgt aacgaagcct tcggaggaga tgcggaaggc
     2341 tatgtatgct gctgaagtcg gcgatgacgt ctatggcgaa gatcccaccg tcaatcgcct
     2401 ggaggagacg gccgccgcgc tcaccggcat ggaggccgcc ctgtttctgc cctcaggaac
     2461 catggggaac ctggtggcgg tgctcacgca ctgcggcaga ggggacggcg cgattctcgg
     2521 cgcggattgc cacatactga attatgaagg cggcggtctg gccgctctcg gctccgtcgt
     2581 tccgctggtt gccgacgaca gctcgggggt gatctctccg gaggaagtgc gcacacactg
     2641 tcgtccgtcg aatgttcact tcgcccccgc caggctgctc tgccttgaga acacgcataa
     2701 ccgcagagga ggactggcgc tctctcccga ggccgttcgg tcttcggcgc aggaagccca
     2761 tcgtctcggt ctctccgtgc atatcgacgg agcgaggatc ttcaacgcat ccacggcatg
     2821 gagctgttct gtgaagaagt acgcggaaag cgtggattcg cttcagttct gcatctcaaa
     2881 ggggcttggc gcaccggtcg gaagccttct gtgcgggagg cgcagctttg tcgacgccgc
     2941 ccgccattgg aggaaacgtc ttggcggcgg actgcggcag tccggcattc ttgctgccgc
     3001 cggtctgtac gcgctggagc ataatatcga acgtctccgg gaggatcacg agaatgccga
     3061 tctgctcgcg catcttctct ccgagggaac cgtcgtcaag gtcgagcaca atgagaaacc
     3121 gacgaacatg gtgtactgcg ccgttcccga tggcgcgtgc gccgatctgc atctccgctg
     3181 cgccgacaga ggcgtcctgt tcaacggcgt tgcggagaac cgtttccgcc ttgtgacgca
     3241 tctcgacgtg tcgagagaac aggtaacccg ggccgcggcg atcatcctcg aggaggctgc
     3301 tgcttcgtga aaaagtttcc ggagaggacg gcggccgaat ctgccgcctc cttcctgatc
     3361 gaaagagcag taaagagggg ggcgtcggcg gcggacgtgc tgtatacctt caattccggc
     3421 cactcccttt ctctgctcga cggagagccc gaaaaaatat cctccggttt ttccctcggg
     3481 ataggtcttc gttcgctcga ttcggcaggg cggcagggcg tcgcgcatgt caactctctc
     3541 gaccggaatc ttcttgaaga agtggtggaa tggagtttct ccaactgcat gtcctcggag
     3601 ccggatgcgt tcgtcgcatt gtccgacccg atccctgcat gcacggagga tctcgctctc
     3661 actgatgaag cggtactgaa cctctcttcc tcggatcgaa tggcgttctg ccgcgagatg
     3721 tgggagaccg caaggagcgc cgatccccgg gtggtctccg tccgcagcgc ttcctggggc
     3781 gacggagccg gagaggtgct ctacgcctct tcgacagggg cgatgatgtg gtacgaaggg
     3841 gcaagcgcag gatgcggcgt ctctgtcatc ctttcatccg gcgacgagat ggagatgggc
     3901 ggatacgggg acgaaagccg gtttcttgcg tctctgcagc cccgtgcgac agctctggag
     3961 gctgtcaggc gtaccgccat ggtgctcggc ggcgagccgc tgcccacagg gaagtacgat
     4021 ctgctccttg atcccgagtc ttccgcgtca ttcgtcgaag tgctcggcga gctctttctc
     4081 gcctccaaca ttcataagaa caagtcgttc ctgaagggca agctcggcga aaagatcgcc
     4141 tccccggcgc ttaccctcgt cgacgacggt acgctcaaag gaggaatcgg cgccgccccg
     4201 ttcgacggag aaggcattcc gtgctcaagg acctgtctcc tctccggggg agttgtcgaa
     4261 tcgtggctct acaacctgaa gtatgcgcga atcgatgggg ttgcgtctac gggaaacgcc
     4321 gcgcgctccg tctccgggac gcccgatgtg gattgctcga atctcttcct tcttccggga
     4381 aagtggactc ctcaggatct tttgctccag gtggggagcg gtatcatggt gaccgagctg
     4441 ctcggactgc atacgatgaa tcccgtgagc ggtgattttt cgcttgggat caaaggcgtg
     4501 cgtatcaagg acggaacgct ttcggaacct gtagcgggga tgacggttgc cggaaacctc
     4561 aaggatgtac tgcgctcaat cgatctcgtc ggcaacgatt ttaagttttt cgggagcatc
     4621 ggctcctgtt ctctggtgat tcgtgatgtt acggctgccg gaacgtaaag tcctgttgct
     4681 tctcctgttc cttttcgcca tgttccttcc ggcggaggcg gagagcgcgt tgcagctctt
     4741 ttcgggcaat cagtgtctgg ggaatgtcaa ctccttgcag cagggcgcgt tttccctcgt
     4801 ctccgcaacg gacatggcat cgcttctgga gttgccggcc gttgcaaagg atgatacgct
     4861 cgtcgtgacg gcggggagaa acaagataca gctcgtatcg ggcgcctcag cagcatggct
     4921 ggacgtggag ctggttccgc tggccgcttc gtgcgttcag cgaaacggtc agtggttttt
     4981 ggaaagcggc tcggcgctca agctttttca ctctcttctc tcccggtccg ggaaaaacgt
     5041 ctcgctgcgc tggggaactg aagtaagcga cggagcgccc gctcctctcg taacgccccc
     5101 cgagccgacg aaaactccct cttcccccgt tcgcgacgcg tctgccgtct ccgccccgtc
     5161 ttcagcccgt cctcttctga aaggggtccg ctgggggatt gacgacgaca agatcagggt
     5221 tgtactggac cttgaaggga ccggcgaacc tgaagttcag cgcggcgccg gcagcatgaa
     5281 ggtcttcttc tcttcagggc cctggcccgt tcccggaatt ccttcccctt accgggaagt
     5341 gaacgcgggt gcgatgaact tcggcgaccg gctgatgatc gacttcgctt cctcgcttgt
     5401 cgtccacgag atcctctctc tggaagctcc gaagagactt gtcatcgact tcagacgaag
     5461 cgccgcatcg ggagcgccgg tagttcctcc ggtcgttgtg cacgcccccc ggcctactcc
     5521 tctcccgacg ctctctccct tgccaccttc gaccgtggaa acgccccgct ctccttcccg
     5581 gacaggaaag cggattgtcg tcgtcgacgc agggcatggg ggaaaagacc ccggcgccgt
     5641 agcgaataaa atccgggaaa aggatctcaa tctctcggtc tccaggatga ttgtcgctcg
     5701 tttgaaggag ttcgggatag acgcgcggct gacgcgcgac tcggatatct accttaccct
     5761 gcgccgccgc acggagattg cgaacactat gaacgccgat ctcttcatca gtatccacgg
     5821 aaatgcgctt cctccgggga aaagcgcaac cggcatggag atttatctga tggcgcttcc
     5881 gacggacaag gacgcgatgg agcttgcccg cttcgaaaac agggagttgg ccgaggacaa
     5941 cggcgattcg acgaaggccg cggacaaacg gacccagacg cttctggcca tcctcggaaa
     6001 catgcagcag aatgcgaaga taagcgagag caccgggttt gccgagtatc tcttcaacgg
     6061 cggaaaagcg ggaggactca agatgcgccg tgtagcccag gcgccgttct acgtgctcag
     6121 gggggcggcg atgcccgccg tgctcgtcga actgggcttt ttgacggaca ggcaggaggc
     6181 gaagcttctc gcggacaaat cctatcaacg gaagatggcg gatgctctcg caggcgggat
     6241 acgggaatat ctgaaaaaga actgatccaa ggaggacgat catttgatgg ggagaaagta
     6301 tgatgatttc gaagacggtt tcgaagtgcg gagggatgag gctccccgtt cctcgaggcg
     6361 atccgccgca aacagagaag gcggacggaa ggcgccgctc ttcatccgac tgattgcgtg
     6421 gaccggcgta ctggttttct gttttgcagc ggggtatgtg ggcacctccg tcgcgttgcg
     6481 catgctcaac cgccaggata tcctccaaag agaggatgta gcggcgaacc gcggagaagc
     6541 cgaaaaaata ctgaacgagg cgcccaaaga gatccgcctg aacgcccgca aggtggagtt
     6601 tacgctgtac tatccgaaag acgggacgat ctcgtccgag aaggcggagg tgctctccgg
     6661 tatcatggag gacgacatcc gccaggtgat agggaagata ctgtcgacgg tttcgggaaa
     6721 gggagcctcc gacgtcaagc tgctcaacct cttccgctcg ggcgatacgc tctttctcaa
     6781 cttcaacgca cactttcttt cgctgctcaa agcgcaggga gagcagggga gcgcgctcct
     6841 catcacctct gtagtgcgga cgatgaacga aaacttttcg ccgatcgttc aagtgcgctt
     6901 cctcgtcgac ggcaaggagc agaagggcgg cgcgcccgtc gacctcagcg tgccgtggcg
     6961 tctcccgaga ggatgacgcc gcgcacatga gagctcccgg actggaacgc ctcctcttcg
     7021 caagcggcaa cagaggcaag tacgtcgaag tgattgatct tttcgcgccg cttggcgtcg
     7081 acattctcta cggacccgac gtcgtcgtcc ttgacgtcga ggaaacgggg agcagctacg
     7141 ggagcaacgc ctttctcaaa gcgaaggcct atgcggctgc gtccggaatg cccgcgctgg
     7201 ccgacgacag cggcatggaa gtccgggcgt tgaactggga accgggtatt ttctcagccc
     7261 gcgtcgcacc gactgacgga gagagaatag aaacgattct cggacgtatg cgcggcgtgg
     7321 cggaccgcta tgcgcggtat gtggcggcct ttgcgctcta tttcccctcc ggcagttgca
     7381 tcgtgtccga gggcgtctgt cccggaacgc tcggggaagc tcccgcggga gatcgcgggt
     7441 tcggctacga cccggtcttt tcgccctccg ggtacgaccg gaccttcggc gaattgccgg
     7501 acgaggtgaa aagaaaaatt tctcaccggg cagtggcagg atatcgattg atggatatcc
     7561 tttcagggaa ttgtgtggta gaatagcttt tcgtttggat ttggttccca tttttttagg
     7621 aggtgcacga cttgaagacg tttcttggta tcgtccacat tcttctctgc atagctctgt
     7681 ccggagccat ccttctgcag cagagaaagc agggcggttt ctccggtatt ttcggcggag
     7741 gtacccaggc agacatgggc ggaagtcagt ggcagcgttt cacggggctt tccaaggtca
     7801 ccgtgatttt gactgcaatc ttcatggtaa cttcgatcgt cctggttctt ctgtaagaga
     7861 agcccatgga atcccgcagc agagggcaat cctgtccggt gtgtccggtg atggaaagtt
     7921 tttccgacgt ccgtgacttc cgtcctgaag gaggtcgttt gtattccgcc acacacgagg
     7981 aaatcctggg cggatgcacg accgacgtct attttgtaaa gactcgggac gttctccgga
     8041 agagcggaaa actgtccacg gatgtgaccg cggagatctt cgcacgaaag aacggcgtct
     8101 tcgccgggct tgacgaggcg cttttgctcc tcaaagggaa gaatctcgcc gtggaatccc
     8161 tccgcgaggg agaccccttc ggaccgaaag agaccctcat gcggatacag ggctcgtacg
     8221 agctcttcgg aacgtacgaa accgttctcc ttggaatgct ggcgagcgcc tccgcatggg
     8281 cgacagctgc gaaagcgtgt gtcgaagcgg cccggggaaa acctgtactc tgtttcgggg
     8341 ctcgtcatgt ccatccttcg attgcgcccg tgatggaacg tacagcggtg cgcgtgggcg
     8401 gatgttccgg cgcaagctgt attctgggcg cgaagctcgc cggtatcgaa cctgtgggca
     8461 cagtgccgca cgcggcagtc ctcataatgg gcgataccgt cgatttggcg atggagtacg
     8521 atgcggtgat gccctccgga gatccgagga ttgtt
//
LOCUS       DAOPKU010000052         8444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8926_length_8444_cov_5.130051, whole genome shotgun sequence.
ACCESSION   DAOPKU010000052 DAOPKU010000000
VERSION     DAOPKU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8926_length_8444_cov_5.130051"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="PK849_02685"
     CDS             complement(<1..1422)
                     /locus_tag="PK849_02685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE65061.1"
                     /translation="MHRLSAKEGGDAVLKTAYEEYLRTGAHGMKIEDGAEAFFVRMEE
                     YRLNGLDLLISSAIPERIFTAGVFSSMRLALALVILVLLAAITLYSALASAFMRPMEA
                     LIDAAEQFSRGDLEKRATVVRDDEIGRLSRTFNAMADNIYLLVGTLESKVKERTAKLE
                     KANAALNESEDRLRLILDSTAEAIYGIDAEGRCTFANASCLRMLGYGREEELLGRDMH
                     ALIHYSQRDGRPIRQDECRIFQVLGMGESAFADDEVFWRADGDSFDVEYSAYPQYKDG
                     KLVGAVVAFMDNTERRKNRAHIDFLTYHDALTGLYNRMYFERALKSLDAEEHLPVAII
                     FGDVNGLKLTNDVFGHAAGDTLLKRSAEILKKVCRESDVVARVGGDEFAVLLPRTGPE
                     AVKKVIERIRNEFGKERILAIKASISMGYAVKTGPDEQLERTVKDAEDAMYKEKTLDR
                     KSVAAEMVRTILETLYARSPREHK"
     assembly_gap    1456..1555
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1556..1947)
                     /locus_tag="PK849_02690"
     CDS             complement(<1556..1947)
                     /locus_tag="PK849_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65062.1"
                     /translation="MLPPTKSVSIKDIILLAFVMLTLITGGISGFIVFSGWLSSAEEN
                     AGRMADEISDVIFSRVNAHFNIPLHINAAYREQLEKGVVDMNNPLQRERFLASVMRAH
                     SGEVIYSFGYATTEGEYYAVRWNERNEL"
     gene            complement(2094..3413)
                     /locus_tag="PK849_02695"
     CDS             complement(2094..3413)
                     /locus_tag="PK849_02695"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HPE65063.1"
                     /translation="MKNTLFAYILLLSLCAVAPAGGEHVPVFPPDSGAQDNGGRLLST
                     LVSLLLPESMELRMDEPPDGAGNVRNISVALRGALLTGFRVERIALESSFLELSPPSS
                     WKPGDRSSLAVRGALRTNTEIVLLEEDINAALEPYATEQYRLAVDLKPGGIRLRVNFT
                     AGHLGGGSLATVDAGLEIRRGSQLWLKNPRILVNGDDQTDAVVNELLKIQPFVDFAAF
                     GFPPNLVAASVDENALRLSSRTSPKPVEGLLYRYEALPGVSPPVVPNAVLKYSPELFR
                     NGDIVFVNGKRWRTKIVRLFDKSPRGFAHAGIVRIVDGLPHVVHASPELGKVGMQSME
                     DFFSFENVDYARVYRFSGDPAVAEAASLRALEYCTREIPFDDRFDTSRTEAMYCTEVI
                     WRAYKAEGIDLARGNWEFARNPIVQGRVLLPYKLSLSPHLEEAFVLE"
     gene            complement(3641..4714)
                     /locus_tag="PK849_02700"
     CDS             complement(3641..4714)
                     /locus_tag="PK849_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65064.1"
                     /translation="MSSVTIRQVTKSFGSNLILSNFDETFKDGEFVTLLGPSGCGKTT
                     MLRMIAGFEKPTTGEIYIDDTLISSDKVFLPPEKRGVGMVFQSYAVWPHMDVFHNVAY
                     PLTIAKKDRASVERKVAHVLDIVHLSQYAKRYPNQLSGGQQQRVALARALVAEPKLLL
                     LDEPLSNLDAKLRESMRFEIKEIQRNLGITVVYVTHDQTEAMAMSDRVFLINRGVVQQ
                     KGTPEEIYRQPVNQFVADFLGKVDFIKGEAGGGRILLHHTEVSLPYAGDKTGAVEVAI
                     RPENITMSKETGDIEGTVTAGYYLGDAGDYRVAVGGISLRVITEGRVFREFTPGDKVF
                     LSLDDFIVYDDDGSLEEQLKINT"
     gene            complement(4730..8113)
                     /locus_tag="PK849_02705"
     CDS             complement(4730..8113)
                     /locus_tag="PK849_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPE65065.1"
                     /translation="MKTLAGADRAIERRTQTALGILLLLTIAMIALGIYGIQALNHSG
                     FDDAKGFGFIRDAGRMAGRNADEDAALLYSLPADAQQAVQGAVDTTIRALWQRVRYAG
                     ETAQDFARADSLKRQGVMQSLLRETQNADLQRSVRPALEALLLMREEIAADGMPAYEK
                     LLKDVAALLPAAEAQSLRVRLADLKKTEAAAVHADIRRRSRALFEEAVAERRLSRFTD
                     GGAGLIPVAKALFEELHAAAPAADLSSAFELVKQVPRQQEAYASLFGLPAHEGTRKIL
                     AMLDAKEIAGARLVAFNKEAYALLKQYHPDINSVDHLPLIAMQAEAMKAEFGDTLSQQ
                     ADLLAARARNDRLSPLLHDAAAAALLSAPREKRALLSSLIAVIEGKDVHVQEDGLVRQ
                     LIAQRDAETAGSIERYLENLQGESALKTKLMLLQTLYVFDAPRLPAAVQDVVGSLPDA
                     ERNTLRLDILRRLSGAPAALPGAVESAFAKDGAELNEQLFHLFLTSRTPQLHVFSEET
                     DTGLRLSIRQNASRVRAAQAVAQGGGNALLQTVNSHGKPLTLLGALLFINVLMLFFLM
                     RSGRDWRFDVKWLLILLIVDFMLVFQILPLGYLLFKAIKPGDTFSFVTFSRLFSYNMN
                     RSALVNTIIGGLAAMVLGTLIAFPLAWMVGRTNMYGKKFFRHVFVLTYMVPPYVGAMA
                     WLRLLNPNVGTINVLLRSFFGITEAAGPINVYSLGGLVWVLTTFYYPYAFITISRAME
                     KMDPSLEEASRISGASPFTTLMRVTLPIMTPSLIAGALLVFVSAASCYGIPSIIGAPG
                     RVHTVTTRIIEYYGRGTQGLNDATGLAVFLMALAILILYLSDFVLAKKQYITVSGKST
                     RPNIVDLRSWRWPLTLLVGLFAVMVVLVPFTTILTTSLKIDVGKPLMAEGNFTFTQWS
                     TIFSRSETMSSLQNSLVYAGVAATAGILVAITMSYLMQRTRIRGRRLPDFLITLGSGS
                     PSVVIALGLIMSMQGRFGVNIYNTAYILIVAYLIKYMMMGMRTVTSAISQIHISLEES
                     SQVSGASWVTTMRRVTGPLIFPSIAAGWFLIFIPCFYELSMTTLLYSNTTKTIGFQLY
                     EYWTYTSQPQASALAFGILLIVILINQVFSRLTKGEFTI"
     gene            complement(8197..>8444)
                     /locus_tag="PK849_02710"
     CDS             complement(8197..>8444)
                     /locus_tag="PK849_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65066.1"
                     /translation="SANMNIAACQAITDWLLSEAGQKFVVAGYMHSVFKGSRDVPFDS
                     VDTNELIKKDIGVDWVRTYKQRNEIQNAFQQSVTVSK"
BASE COUNT         1659 a         2433 c         2581 g         1671 t
ORIGIN      
        1 cttgtgctcc cggggactcc tggcatagag cgtctccaga atcgtgcgca ccatctccgc
       61 cgcgacgctc ttccggtcga gcgtcttctc cttgtacatc gcgtcctcgg cgtccttcac
      121 agtgcgttcc aactgctcgt ccgggcctgt cttcacggca taccccatgg agatgctcgc
      181 ctttatcgca aggatccgct cttttccgaa ctcgttccgg atcctttcga taaccttctt
      241 caccgcctcc ggcccggttc gaggcagcag aacggcaaat tcgtcgcctc ctaccctggc
      301 gacgacgtcg ctctccctgc acactttctt cagtatctcc gccgaccgtt tcaggagcgt
      361 atctcccgcg gcgtgcccga agacgtcgtt cgtcagcttg agaccgttga cgtccccgaa
      421 gatgatcgcg acaggaagat gctcctcggc gtcaaggctt ttcagggctc tctcgaagta
      481 catcctgttg tacaggccgg tgagcgcgtc gtggtaggtg aggaagtcga tgtgcgccct
      541 gtttttcctg cgctcggtat tgtccatgaa ggcgacgacc gcgccgacaa gtttcccgtc
      601 tttgtactgg ggataggccg agtactccac gtcgaatgag tctccgtcgg cccgccagaa
      661 gacttcgtcg tcggcgaagg cgctctcgcc catcccgagc acctgaaaaa ttctgcactc
      721 gtcctggcgg atcggcctgc cgtctctctg cgagtagtgg atgagagcgt gcatatccct
      781 gccgagcagc tcttcctcgc gcccgtaccc cagcatcctc agacagctcg cgttggcgaa
      841 cgtgcaccgt ccctccgcgt cgatgccgta gatagcctcc gccgtggagt cgaggatcag
      901 gcggagacgg tcttcgcttt cgttcagcgc cgcgttcgct ttctccagct tcgcggtgcg
      961 ctccttcacc ttcgattcca gcgtcccgac gagaaggtag atgttgtcgg ccattgcgtt
     1021 gaacgttcgc gaaaggcggc cgatctcgtc gtcgcgcaca acggtcgccc tcttttcgag
     1081 atcgcctctt gagaattgct cggcggcgtc gatcagcgcc tccatggggc gcatgaaggc
     1141 gcttgcaagc gcgctgtaaa gcgtgatcgc ggccaggagt accagaatga ccagcgcaag
     1201 cgccagccgc atgctgctga agacgcccgc ggtaaagatc cgttccggta tggcggaaga
     1261 aatgagaagg tccagcccgt tcagacggta ttcctccata cggacaaaga acgcctcagc
     1321 gccgtcctcg atcttcatgc cgtgcgcccc cgtgcgaaga tactcctcgt aggcggtctt
     1381 cagtacggcg tccccgcctt ctttcgcaga gagtctgtgc acgcgcccat ccgggaggag
     1441 agaaaagttc ggcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntccag
     1561 ctcgttccgt tcattccacc ggacggcgta gtattccccc tccgttgtgg cgtagccgaa
     1621 gctgtagata acctcgcccg aatgcgcgcg catcacggaa gccagaaagc gctcccgctg
     1681 cagcggattg ttcatgtcga cgacgccttt ttccagctgt tcgcggtagg cggcgttgat
     1741 atgcagggga atgttgaaat gcgcgttcac cctgctgaag atgacgtcgc ttatctcgtc
     1801 ggccatccgg ccggcgttct cctcggcgga cgagagccat ccggagaaga cgatgaagcc
     1861 cgaaatcccg ccggtgatca gcgtcagcat cacgaacgcc aaaaggatga tgtcttttat
     1921 tgagacgctc ttcgtcggcg gaagcatggg ctcacctcga tcattccggg ctcttcatta
     1981 tgaaggcagt tgtaattata cagacaaaat tcccttttat tcaaccgctc gttccgtcgt
     2041 gaccgtgata ctgtgtaaat gtgtattctt gcagttcgaa aaaggacgag gcgctactcg
     2101 agcacgaacg cttcctccag atgcgggctc agactcagtt tgtagggaag gagcaccctc
     2161 ccctggacga tggggttgcg cgcgaactcc cagtttcccc gggccagatc gatcccctcc
     2221 gccttgtagg cgcgccagat cacctcggtg cagtacatgg cttctgttct cgacgtatcg
     2281 aaccggtcgt cgaaggggat ttcgcgcgta cagtattcga gcgcccgcag gctcgccgcc
     2341 tccgcgacgg cgggatcgcc ggagaagcgg tatacccgcg cgtagtccac attctcgaag
     2401 gagaagaagt cctccatcga ctgcatcccc accttgccca gctcgggcga cgcgtggacg
     2461 acatgcggaa gaccgtcgac gatgcgcacg attccggcgt gagcgaatcc gcgggggctt
     2521 ttgtcgaaga ggcggacaat cttcgttctc cacctcttgc cgttgacgaa gacgatgtcc
     2581 ccgttgcgga agagttccgg cgaatacttc agaaccgcat tcggcacgac gggaggagag
     2641 actcccggga gcgcctcgta acggtagagc agcccttcga cgggcttcgg agaagtccgg
     2701 gaggagagtc ggagcgcgtt ctcgtccacg gaggcggcga caagattcgg cggaaagccg
     2761 aaagcggcga agtcgacgaa gggctgaatc ttcagcagct cgttcactac cgcgtccgtc
     2821 tggtcgtcgc cgttgacgag gatacgcgga ttcttcagcc agagctggct cccccttctg
     2881 atttcaagtc ctgcgtccac cgttgccaga ctcccgcccc ccagatgccc cgccgtgaag
     2941 ttgacacgaa ggcggatacc tccgggcttg agatcgactg cgagtcggta ctgctccgtc
     3001 gcgtacggtt cgagcgcagc gttgatatcc tcttcaagga ggactatttc cgtgttcgtc
     3061 cgcagcgcgc ctcgcacggc aagcgagctc ctgtcgccgg gcttccacga cgacggggga
     3121 gagagctcca ggaaggagga ctcaagcgcg atccgctcca cgcggaagcc ggtcagcaag
     3181 gctccgcgca gcgcaacgga aatgttgcgg acattccccg cgccgtccgg gggttcgtcc
     3241 atgcggagtt ccatcgactc gggaagcagc agcgatacca gcgtcgaaag aagacgcccg
     3301 ccgttgtcct gcgctccgga atccggcggg aagaccggta cgtgctcccc tcccgccggg
     3361 gcgacggcgc agagggaaag aaggagtatg tacgcgaaaa gggtgttttt cacacggcac
     3421 ctccttgaca tgttcgtttc cgttgatcta tcgacgatga ttcttccggc tgcgcagggg
     3481 aattggcgga aatcatgaag gaaaaggggc tgagagcgat gaccgcgtgg aaaaacaagg
     3541 cggccaaagc aagagcgtat cgcccagtat gcaacggcgt cacgacgtag taccccttcc
     3601 cggcgccgcg cacaaaagag cacgaagctt caagggaaca tcaggtgttt atctttaact
     3661 gctcctccaa ggagccatcg tcgtcgtaga cgataaagtc atccaaggag agaaagacct
     3721 tgtcgcccgg cgtgaactcg cggaagacgc gcccctcggt aatgacgcgc agggaaatgc
     3781 cgcccacggc gacccggtag tcgccggcgt cgccaaggta gtatccggcg gttaccgtac
     3841 cctcaatgtc gcccgtttcc ttggacatgg tgatgttttc ggggcggatg gcaacttcta
     3901 cggcgcctgt tttgtcgccc gcatacggca gcgatacctc ggtgtggtgc aaaagaatgc
     3961 gtccgccgcc ggcctcgccc ttgataaagt ccaccttgcc caggaagtcc gcgacgaact
     4021 ggttgacggg ctgacggtag atttcttcgg gagtgccctt ttgctgcacg acgccgcggt
     4081 tgatcaggaa cacgcggtcg ctcatggcca tggcctcggt ctggtcgtgg gtgacgtaca
     4141 ccaccgtgat gcccagattg cgttgaatct ctttgatttc aaagcgcatt gattcgcgca
     4201 gtttagcgtc caggttactc agcggctcgt ccagcagcaa gagcttgggt tcggccacca
     4261 gggcgcgggc gagggcgacg cgctgctgct gtccgcccga caactggttg gggtagcgtt
     4321 tggcatactg tgacaggtgc acgatgtcca gcacgtgcgc caccttccgc tcgacggagg
     4381 ctctgtcctt cttggcgatg gtcagcgggt aggccacgtt gtgaaagacg tccatgtgcg
     4441 gccagacggc atagctttgg aacaccatgc ccacgccccg tttttcgggc ggcagaaaca
     4501 ccttgtcgga gctgatcagc gtatcgtcga tataaatttc cccggtggtg ggtttttcaa
     4561 accctgcaat catgcgcagc atggtggttt tgccgcaacc ggagggcccc agcagggtaa
     4621 cgaactcgcc gtctttgaac gtttcatcga agttgctcag aatcaggttg ctgccgaagg
     4681 acttggtgac ttgacggatt gttacgctcg acataatgcg gctcctctat caaatagtga
     4741 actcgccctt ggtcaggcgg ctgaaaacct ggttgatcaa aatgacaatg agcaggatac
     4801 caaaagccag ggcgctggcc tggggctggc tggtataggt ccaatactcg tacagctgaa
     4861 agccgatggt cttggtggtg ttggagtaaa ggagcgtggt catggacaac tcatagaagc
     4921 agggaataaa gatgaggaac cagcccgccg cgatggaggg aaagatgagc ggtcctgtga
     4981 cacgccgcat ggtcgtcacc caggaggcgc ccgacacctg cgaggattct tccaacgaga
     5041 tgtggatctg gctgattgcg ctggtgacgg tacgcatgcc catcatcatg tacttaatca
     5101 gatacgcgac gatgaggata taggcggtgt tatagatgtt gacgccgaag cgcccctgca
     5161 tgctcatgat aagccccagg gcaatgacca cggaggggga acccgagccc agtgtgatta
     5221 agaagtcggg caggcggcgt ccccgtatgc gggtgcgctg catcaggtag ctcatggtga
     5281 tggccaccag tatgcccgcc gtagcggcca ccccggcgta cacaaggctg ttttgcagcg
     5341 agctcatggt ctcggagcgg gagaagatgg tgctccactg ggtgaaggtg aagttgcctt
     5401 ccgccatgag cggcttgccc acgtcgattt tgagcgaggt ggtgaggatg gtggtgaagg
     5461 gtaccagcac caccatcacg gcgaaaaggc ctaccagcag cgtcagaggc caacgccagg
     5521 accgcagatc gacaatgttg gggcgggtgg acttgcccga aaccgtgatg tactgttttt
     5581 tggccagcac aaagtcggac agatagagta tcaggatagc cagcgccatc aaaaagacgg
     5641 ccagaccggt ggcgtcgtta agcccctgcg tgccgcgacc gtagtattct atgatgcggg
     5701 tagtgacggt atgcacgcgg ccgggcgcgc cgatgatgga agggatgccg tagcagctgg
     5761 ccgcgctgac gaacaccagc agagcgcccg caatcaggga gggcgtcatg atgggcagtg
     5821 taacgcgcat cagcgtggtg aagggagaag cgcccgagat gcgggatgct tcttccaggg
     5881 aggggtccat cttctccatc gcacgggaaa tggtgataaa agcgtagggg tagtagaagg
     5941 tggtcagcac ccagaccagg ccgcccagcg agtacacgtt gatagggccg gcggcctcgg
     6001 tgatgccgaa gaaacttctc agcagcacgt tgatcgtgcc cacattgggg ttcagcaggc
     6061 gcagccaggc catggcgccc acgtagggcg gcaccatgta ggtaagcacg aacacgtggc
     6121 ggaagaactt cttgccgtac atgttggtgc gccccaccat ccacgcgagc gggaaggcga
     6181 tcagggtgcc cagtaccatg gcggcgaggc cgcctataat ggtgttgacc agcgcgctgc
     6241 ggttcatatt gtagctgaac aggcggctga aggtgacaaa ggaaaaggtg tcgcccggct
     6301 taatggcctt gaacaaaagg tagcccagcg gcaatatctg gaaaaccagc ataaagtcga
     6361 caatcagcag aatcaacagc catttgacat cgaagcgcca gtcgcggccc gagcgcatca
     6421 ggaaaaacag catcaggacg ttgataaaga gcagggcgcc cagcagcgtc agcggctttc
     6481 cgtggctgtt gacggtttgc aagagcgcgt tgccgccgcc ctgggccacc gcctgcgcgg
     6541 cacgcacgcg tgaggcgttc tggcggatgg aaaggcgtag gccggtatcg gtttcctcac
     6601 tgaacacatg caactgtggg gtgcggctgg ttaaaaagag gtggaacagc tgttcgttga
     6661 gctcggcgcc gtccttggcg aaggcgcttt ccactgcccc gggcagggcg gcgggcgcgc
     6721 cggacagacg gcgcaatatg tccagcctga gcgtattacg ttccgcgtcg gggagggatc
     6781 ccaccacatc ttgcacggcg gcgggcaggc gcggcgcgtc aaacacatac agagtttgca
     6841 gcagcatgag ctttgttttc agggcgcttt ccccctgcaa attttccagg tatctctcga
     6901 tgctgccggc ggtctcggcg tcgcgctgcg caatcagctg gcggacgagc ccgtcttcct
     6961 gaacatgtac atccttgccc tcgatgacgg cgatgaggga agagagcaga gcgcgcttct
     7021 cgcggggcgc cgaaagcaga gcggcggcgg cggcatcgtg caacaggggc gacagtctgt
     7081 cattccgtgc gcgggcagcc aataaatccg cctgttggct gagcgtatcg ccgaactcgg
     7141 ccttcatggc ttcggcctgc atggcgataa gcggcagatg gtcgacgctg ttgatatcgg
     7201 ggtggtattg cttgagcagc gcatacgcct ctttgttgaa ggcaacaagg cgcgcgccgg
     7261 caatctcctt ggcgtcaagc attgcgagaa tttttcgcgt gccctcgtgc gcgggtaaac
     7321 cgaacagcga ggcataggct tcctgctgcc tggggacctg cttgacaagt tcgaaggccg
     7381 aggatagatc ggcggcggga gcggcggcat gcaactcctc aaacaaggct ttggcgacgg
     7441 gaatcagccc ggcgcctccg tccgtaaagc gggacaggcg cctttccgcg acggcctctt
     7501 caaacagggc ccgggagcgg cgccggatgt cggcgtgcac ggcggcggcc tctgtcttct
     7561 tgagatcggc aaggcgcacc cgcagggact gcgcctctgc cgcgggcagg agggcggcga
     7621 catccttcaa cagcttttca taagcgggca ttccgtccgc cgctatctct tcgcgcatca
     7681 acaacagggc ttcaagcgca gggcggacgc tccgctgcaa atccgcattc tgcgtttcgc
     7741 gcaacagcga ctgcatgacg ccctgacgtt ttaggctgtc ggcgcgcgca aaatcctgcg
     7801 cggtttcgcc ggcgtagcgc acacgctgcc ataaggcgcg gatggtcgta tccacggcgc
     7861 cttggacggc ctgctgcgcg tctgcgggaa gcgaatacaa cagcgcggca tcttcgtctg
     7921 cgtttctgcc ggccattcgc ccggcgtcgc ggataaaccc gaagcccttt gcgtcgtcaa
     7981 agccggagtg gttgagagcc tgtatgccat agatgccaag ggcaatcatt gcgatggtca
     8041 gcagcaataa aatgcccagt gctgtttgag tgcgacgttc gatagcgcgg tcggcgccgg
     8101 caagtgtttt catggtgtat atcctctaaa aagggcgaag gaaaacctcg ttctgccggt
     8161 ctgcctccgc ccgattactg tgcaatagag cttcgtttat ttggaaacgg tcacgctttg
     8221 ctggaaggcg ttctggattt cgttgcgctg cttgtaggta cgcacccagt cgacgccgat
     8281 atccttctta atcaattcgt tggtatcgac ggagtcgaaa ggaacgtcgc ggctgccctt
     8341 gaacacagag tgcatatagc cggccaccac gaacttctgg cccgcttcgg acagcaacca
     8401 gtcggtgatt gcctggcagg cggcgatgtt catgttggcg ctgc
//
LOCUS       DAOPKU010000053         8404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_8986_length_8404_cov_4.073302, whole genome shotgun sequence.
ACCESSION   DAOPKU010000053 DAOPKU010000000
VERSION     DAOPKU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8986_length_8404_cov_4.073302"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..757
                     /gene="jag"
                     /locus_tag="PK849_02715"
     CDS             107..757
                     /gene="jag"
                     /locus_tag="PK849_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPE65067.1"
                     /translation="MNDDILVLDVSSVEEARKIASAEWDLDSADLTIKVIEEEKSFFG
                     LLGRKLRVEVRPVAPLHVLRGRNTIDSLLKMMELHITSEIADENRINLSGPDAGIIIG
                     KYGETLKSIEYLLNLVSRGADEGRRIVLDSDGYRERRELSLQRLALAAARKCARKGRP
                     AHLEPMTSWERRIVHLTLKDSPDVETRSVGESPSRKVVIYPLRRSSYSGRPPRKDS"
     gene            757..1536
                     /gene="lgt"
                     /locus_tag="PK849_02720"
     CDS             757..1536
                     /gene="lgt"
                     /locus_tag="PK849_02720"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPE65068.1"
                     /translation="MYPVLFHIGDNPVHSYYLLWTLALTLAVVFSRRRMTCSYGIDDD
                     DARSVIIWAFIGMLLGARAGSVYDSWSIYSADPLKILRIWEGGLSAVPAFLGAGAVSF
                     VYSKRRKIPYWMIVDAGALPAALTVAIGRWGCFLNGCCTGIETTVSWGVRFPSDALGL
                     LRHPTQLYYSFGALFIAALLQWTESSWLGYHNDRRIRGAVLCPLFAILYSLLRLAADP
                     FRSDFTRIGMQTNRSVLFVVLGISMLWLGYSVFYEKKCIRS"
     gene            1631..3049
                     /locus_tag="PK849_02725"
     CDS             1631..3049
                     /locus_tag="PK849_02725"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPE65069.1"
                     /translation="MKGTKITSAFFGSVKVLFVLLLLVVPFLLTADRAFAQDPFTGNP
                     VAKIARELSPAVVNIDVETMVTRSVHPFADDPFFRQFFGEEQRRFSRTVPMKGRGSGF
                     IVSKDGHIITNNHVIDGADKITVTFSDGRTFEAKVLGKDPTFDLAVIKIDGADLPFLE
                     LGDSDATEVGEWVVAIGNPFGLEHTVTVGVVSAKNRSIHAGNVNFDGFLQTDAAINPG
                     NSGGPLLNLEGKVVGINTAIVPFAQGIGFAVPVNMAKQVMDDLIEFGRVKRAWLGVYI
                     QPLTKEFAEAYGVKEENGAVVSDVLRDSPAEKAGLQRGDVILEVDGSGVKNHQEFVMK
                     IRQKLAGDKVTLTLIRQKKNIKVDIPLGEMPGNEKQAASRTGGSKMLTSIGLEAGEMT
                     DDIRKQNNLRKDEGVLITAVEDGSMAQRAGIRENDVLLEVNGVRLSGLSDLDKADVKG
                     NSVVLLIMRDSRSFFVSIRTGQ"
     gene            complement(3125..3802)
                     /locus_tag="PK849_02730"
     CDS             complement(3125..3802)
                     /locus_tag="PK849_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE65070.1"
                     /translation="MSVLIIEDNRDLVQVLAEVLNENGFSVESAHTGEEGLEKALLSR
                     FDCIILDIVLPGINGFQVVEEYRKEDGSSPVLILSARDSVEDKVEGFNRGADDYMVKP
                     FDSRELIVRVRSLIRRAADKQSPLLACGRLTLDPVTRECRVDGTFVNLRRREFDILET
                     MLRHANQVFTRERLVASVWKKEYDGRSNVVDVHVKYLRDKLRAHGFDTAIETIRGVGY
                     KIRCSEE"
     gene            4062..4328
                     /gene="rpsO"
                     /locus_tag="PK849_02735"
     CDS             4062..4328
                     /gene="rpsO"
                     /locus_tag="PK849_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPE65071.1"
                     /translation="MLQGEKKQGIITEFQVHTTDTGSPEVQVAMLTQRIRELTEHLKI
                     HTKDFHSRRGLLKMVGKRRRLLRYLKDKDFNRYRTLIERLGLRH"
     gene            4443..>5174
                     /locus_tag="PK849_02740"
     CDS             4443..>5174
                     /locus_tag="PK849_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPE65072.1"
                     /translation="MEMTFSVEIGGRPLEFSTGKVAKQAGGAVVAAHGETTVLTTACI
                     TDKPRTGIDFFPLLVDFEERFYSAGKIPGGFIKREGRPSETAILSCRMVDRSIRSLFD
                     ETMRHDVHVVSTVLSVDQVNPPNVLAINAASAALTISDIPWGGPVGAVRIGRLGDEFV
                     VNPTEEQMLESTLDLLVAGHLDGVTMVECGSKEIAEDLLVDALELAQNEIKKIVGLFN
                     EMRAAVGREKLVLPSAPSFPDIDAWA"
     assembly_gap    5175..5274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5275..6588
                     /gene="pnp"
                     /locus_tag="PK849_02745"
     CDS             <5275..6588
                     /gene="pnp"
                     /locus_tag="PK849_02745"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPE65073.1"
                     /translation="TCETGVLPRVHGSALFTRGETQALVVTTLGMMGVDDQILDGLKQ
                     DEPAKRFILHYNFPPFSVGEVRPMRGPGRREIGHGALAERALRSMIPDEADFPYVMRV
                     VSGILESNGSSSQASICGGSLALMSAGVPMKKHVAGIAMGLIKEGEKVAVLTDIQGLE
                     DHFGDMDFKVAGTRDGVTALQMDNKAGGITRAILEQALSQARAARMQILDSMEAAIPV
                     PAELSANAPRIFMTTIDPEKIRDVIGPGGKVIRGITQKTGIKINVEDSGEIYIGGSSQ
                     EKVDEALSIIRGLTKDLEAGEVYHGTVTRLMTFGVFIECLPGKEGLLHVSEVSTHRIP
                     KVEDVFKVGDKVLVMVKEIDDMGRTNLTRRRILENESRIAEEGLSEVLPAEQERESVI
                     AALAEANKNNPQPERERPRDGGGRGDRRPGGGGSGPRRHSSGGRP"
     gene            6595..7038
                     /gene="dut"
                     /locus_tag="PK849_02750"
     CDS             6595..7038
                     /gene="dut"
                     /locus_tag="PK849_02750"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPE65074.1"
                     /translation="MPVAVKILREGRAKEFPLPEYATESAAGADLRSAEDMLLLPGER
                     TSVGTGIRIELPEGYEAQIRPRSGLAIRHGVTILNAPGTIDSDYRGEIRILLVNLGKD
                     PFTIVAGDRIAQLVVAPVARVEWREDETLGLTGRGEGGFGSTGKS"
     gene            7358..>8404
                     /locus_tag="PK849_02755"
     CDS             7358..>8404
                     /locus_tag="PK849_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPE65075.1"
                     /translation="MMQFTGNNGKQAELASGTAGDVLDALGMKKGAIAAKIDGCAVDL
                     SRVVERGGEAAPILPESDEGLDILRHSAAHLMAQAVAQMYPGTRYGVGPAIKDGFYYD
                     MEIPGGITEEDLPKIEKEMHRIAKRSVPVERRVLSKAEALEWFKAKNDPYKVEIISEL
                     EDETVTLYVQGEYADLCRGPHVPNTSYVRHFKLLSVAGAYWRGDEKNIMLTRVYGTAF
                     GDEESLAAYIHRMEEAKNRDHRRLGKELDLFSLQAEGPGFPFFHPKGMVILNNLMDFW
                     KKEHTKRGYCEIRTPLILERSLWIQSGHWDHYRDNMYFTEIDERPFAIKPMNCPGGML
                     VYKSQIRSYRDLPLR"
BASE COUNT         1806 a         2300 c         2491 g         1707 t
ORIGIN      
        1 tcggggtgtc cgcgtacgtc gtgcagcata cagccacttc ttctcctttt caggggaacg
       61 gagaggcttt ctcccaggga gttctctgaa cggaaggagg attgttgtga acgacgacat
      121 acttgttctg gacgtttctt cggtggaaga ggcccggaaa atagcttcag ccgagtggga
      181 tcttgactct gctgatctga caatcaaggt aatcgaggag gagaagagtt ttttcggtct
      241 cctcggcaga aaattgcgcg tggaggtgcg tccggtcgcc cctcttcacg ttctccgggg
      301 cagaaatacc atcgatagcc tgctgaagat gatggaactg catattacct cggaaatagc
      361 cgatgaaaac aggatcaatc tctccggacc ggatgcgggc atcatcatcg ggaagtacgg
      421 agagacgctc aagtcgattg agtacctgct caatcttgtc tcaaggggag cggacgaggg
      481 gcgccgcatt gttctggaca gcgacgggta cagagagcgg cgagagctga gccttcagcg
      541 cctcgcgctg gccgccgcgc gcaagtgcgc ccggaagggg agacccgcgc accttgaacc
      601 gatgacgagc tgggaacggc ggatcgtcca tcttacgctg aaagacagcc cggatgtgga
      661 aacgaggtcc gtaggcgaat cgccctcgcg aaaggttgtc atttatccgc ttcggaggag
      721 ttcgtacagc ggaagacccc cccgaaagga ttcctgatgt acccggttct ctttcatatc
      781 ggcgacaacc cggtccacag ctactacctg ctgtggacct tggctttgac tctggccgtc
      841 gttttttcgc gccggagaat gacctgttcc tacggaatag atgacgacga tgcgcgctcg
      901 gtgattatct gggccttcat tggaatgctt ctgggcgctc gtgcgggaag cgtctacgac
      961 tcatggtcca tctatagcgc ggaccctctg aagattctca gaatatggga gggagggctt
     1021 tccgccgtcc ccgcctttct gggcgccggg gccgtttcct tcgtctattc aaagcgccgg
     1081 aaaatcccgt actggatgat cgtggacgcc ggagcgcttc cggcagccct caccgtcgca
     1141 ataggccgct ggggctgttt tttgaacggg tgctgcacag ggatcgagac gacggtttca
     1201 tggggagtcc gtttcccctc ggatgctttg gggcttctcc gccatccaac gcagctctac
     1261 tactctttcg gagcgctttt catcgccgct cttctccagt ggacggaatc ctcgtggctt
     1321 ggctaccata atgaccgcag aatccgggga gcggttctat gtcctctctt cgcgatactt
     1381 tattctctgc tgcggctggc tgccgatccc ttccggagcg actttacccg catcgggatg
     1441 cagacgaacc gctctgttct cttcgttgtg ctcggaataa gcatgctctg gctcgggtat
     1501 tccgtattct acgagaaaaa atgtatacga agttgatatg aaatgcgtat tcatggatag
     1561 gtaaaaaaaa cgatgtatcc agccgccggg cgcatacaat aaaagcatct tgattcggag
     1621 gtgacacacc atgaaaggaa caaaaattac gagtgcgttc ttcggttccg tgaaagtttt
     1681 gtttgtgctt cttctgcttg tcgtgccctt tttgctgacg gcggatcggg cattcgcgca
     1741 agatccgttt accgggaacc ctgtcgccaa aatagcaagg gagctttctc ccgcggtggt
     1801 caatatcgat gtggagacga tggtcacccg ttccgtccat ccgttcgcgg acgacccgtt
     1861 tttccgccag ttcttcggtg aggagcagcg ccgtttctcc cgtaccgttc ccatgaaagg
     1921 gagaggctcc gggttcatcg tgtcgaagga cggacatatc ataacgaata accacgtcat
     1981 cgacggcgcc gacaagataa cggtgacctt ctcggacggc cgtacgttcg aggcgaaggt
     2041 attgggcaag gatcctacat tcgatcttgc cgtcatcaag atcgacggtg cagacctccc
     2101 gtttcttgaa ctcggcgatt cggacgcgac cgaggtcggc gagtgggtag tcgcgatagg
     2161 aaatccgttc ggacttgaac atacggttac cgtcggtgtc gtctcggcga agaaccggag
     2221 cattcatgcc ggaaacgtga acttcgacgg gttccttcag acggatgccg cgatcaaccc
     2281 cggcaatagc ggcggtcctc ttctgaacct ggaagggaag gtcgtcggaa tcaacaccgc
     2341 catcgttccg ttcgcccagg gtatcggctt cgctgtgccg gtgaatatgg cgaagcaggt
     2401 catggacgat ctcattgagt tcggccgggt gaagagagca tggctcggcg tgtacattca
     2461 accgctgacc aaggagtttg ccgaggcata cggtgtgaag gaagagaacg gggccgttgt
     2521 cagcgacgtg cttcgcgact cacccgccga aaaagccggg ctccagcggg gcgatgtcat
     2581 ccttgaggtt gacggttccg gcgtgaagaa tcaccaggag ttcgtgatga agattcggca
     2641 gaaactcgcc ggagacaaag ttaccctgac gcttatccgc cagaagaaga atatcaaggt
     2701 ggatatcccc ctcggcgaga tgcccggcaa cgaaaaacag gccgcttccc gcaccggcgg
     2761 atcgaagatg ctcacctcta tcggattgga agccggcgag atgacggatg atatccggaa
     2821 gcagaataac cttcgtaagg atgagggtgt gctgatcacc gccgtcgagg acggctccat
     2881 ggcgcagaga gcgggtatcc gcgagaacga cgtgcttctg gaagtgaacg gcgtccgtct
     2941 ttccggcctc tcggatctcg acaaggcgga tgtcaagggc aattccgttg tgctgctgat
     3001 tatgagggat tcgagatcct tcttcgtctc catacggaca gggcaataaa gaagagtcag
     3061 taaaagaaga agagcgaagc gcccctgcac gggcgcttcg ctcttcttct ggagtaggtt
     3121 tctcctactc ttcggaacaa cgaatcttgt atcccacacc tcgtatcgtt tcgatggcag
     3181 tatcaaatcc gtgagccctc agcttgtccc tgagatactt gacatggacg tcgacaacgt
     3241 tgctcctgcc gtcgtattct tttttccaca cggatgcgac aagacgttcc cttgtgaaca
     3301 cctggttggc gtgccggagc attgtttcaa gaatatcgaa ctccctgcgt ctgaggttga
     3361 cgaaagttcc gtccaccctg cactcccgcg tcaccggatc gagcgtgagc cgaccgcagg
     3421 caagcagcgg cgactgtttg tcggcggctc tccggatgag cgatcgtacc cgcacgatga
     3481 gttctcttga gtcgaacggt ttcaccatgt agtcgtcggc tccgcggttg aacccctcca
     3541 ccttgtcctc caccgagtcg cgggcggaca aaatgagtac cggcgaactt ccgtcttcct
     3601 ttctgtattc ctccacgacc tgaaacccgt tgatccccgg gaggacgata tccaaaatga
     3661 tacagtcgaa tctcgagagc agcgcttttt caagcccttc ttcccctgtg tgcgcgctct
     3721 caacggaaaa accgttttcg ttgagcactt cggcgagcac ttgcactaga tctctgttgt
     3781 cctcgatgat gagaacggac acgttgcttc ccccctaatg aaaaaactct ggtaaaataa
     3841 tcaaaaagat taaagcacat ccagaattaa aagtcgagtg caagatgaga tcactttttc
     3901 tttttctgaa atccagctga aatttgctct cttcccgctg ggcgtgcgtg atgatttgct
     3961 ttattttctg caagatgata gtattactgg cgacccttac ccggatttgc gcatagagcg
     4021 ctcttctgag ttacgggatt ttgtctacga ggtgaagaaa catgttgcag ggagagaaga
     4081 aacagggaat tattacggag tttcaggtgc acacgaccga caccgggtcg cccgaggttc
     4141 aggtggcgat gctcacgcag cgcattcgcg agcttaccga acatctcaag attcacacga
     4201 aggacttcca ttctcgccgt ggtcttctga agatggtcgg caaacgccgc agacttctcc
     4261 ggtatctgaa ggacaaggat ttcaaccggt acagaacgct tatcgagaga ctcggtctgc
     4321 gtcactaaaa aaggtcgatt tccggtgcga gggcgaaaag tcctcgcact ttttttgttc
     4381 gattgtggta ttcttactcc gcatacaata aaaagagctg aattttgagg aggaagaagt
     4441 gtatggaaat gacgttttca gttgaaattg gaggccgtcc tctggagttt tccaccggaa
     4501 aagtggcaaa gcaggcaggc ggagccgttg tggcggcaca tggcgaaacg acggtgctta
     4561 ccacagcctg catcaccgac aaaccgcgca ccgggataga ttttttcccg ctgcttgttg
     4621 attttgaaga gagattttac tccgccggga agattcccgg agggtttatt aagcgcgaag
     4681 gtcgcccctc cgaaacggcc attttgagct gccggatggt cgaccgttcc atccgctcgc
     4741 tttttgacga gacgatgcgc cacgacgtcc atgtggtttc gacggtgctc tcggtcgacc
     4801 aggtgaaccc gcccaacgtg ctcgcaatca acgccgcatc cgccgcgctc accatctcgg
     4861 acatcccgtg gggaggcccg gttggagccg taaggatcgg ccgtctcggc gacgaattcg
     4921 tggtgaaccc caccgaggag cagatgctcg aatccaccct cgaccttctg gtcgcaggac
     4981 atctcgacgg agtgacgatg gtcgagtgcg gttcgaagga gatcgcggaa gacctcctgg
     5041 tcgacgccct tgagctggcg cagaacgaga tcaagaagat cgtcggcctc ttcaacgaga
     5101 tgcgcgccgc tgtaggacgc gagaagcttg tacttccgtc agctccctcc ttccccgaca
     5161 tcgacgcatg ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacctgc
     5281 gagacgggcg ttcttccgag agtgcacggc tccgcgctct ttacccgcgg cgagacgcag
     5341 gcgctcgttg tgacgacgct cggcatgatg ggtgtggacg accagatact cgacggactg
     5401 aaacaggacg agcccgccaa gcggttcatt ctgcactata acttcccccc gttctccgtc
     5461 ggagaggttc gtcccatgag aggaccggga cggcgcgaga tagggcacgg cgcgctcgcg
     5521 gagagggcgc ttcgctccat gataccggac gaagccgact tcccctacgt gatgcgcgtg
     5581 gtctcgggta tcctcgagtc caacggctcc agctcccagg cgtccatctg cggcggcagc
     5641 ctcgctctga tgagcgcggg cgttccgatg aagaagcacg tcgctggaat cgcgatgggt
     5701 ctcatcaagg aaggcgagaa ggttgcggtg cttaccgata ttcaggggct tgaagatcac
     5761 ttcggcgaca tggacttcaa ggttgccgga acccgcgacg gcgtgaccgc tctccagatg
     5821 gacaacaagg ccggcgggat caccagggcg attcttgagc aggcgctctc ccaggcccgt
     5881 gcggcgagga tgcagatcct cgacagcatg gaggccgcga tccccgttcc cgcggagctt
     5941 tccgcgaacg cgcccagaat cttcatgacc actatcgacc ccgaaaaaat ccgcgacgtc
     6001 atcggccccg gcggcaaggt tatccgcggc attacgcaga agacgggcat caagatcaac
     6061 gtcgaggaca gcggcgaaat ctacatcggc ggctcctctc aggaaaaggt cgacgaggcg
     6121 ctctccatca tccgcggcct cacgaaggac cttgaggcag gagaggtcta ccacggcacc
     6181 gtgacccgtc tcatgacctt cggcgtcttc atcgagtgcc ttccgggcaa ggaagggctg
     6241 cttcatgtga gcgaggtcag cacccaccgc atcccgaagg tggaggatgt cttcaaggta
     6301 ggcgacaagg tgctcgtcat ggtgaaggag atagacgaca tggggcgcac caacctgacg
     6361 cgcaggcgca tcctcgaaaa cgagagccgc atcgccgaag aaggcctctc ggaggttctg
     6421 cccgccgagc aggagcgcga gagcgtgatc gccgcgcttg cggaggcgaa caagaacaat
     6481 cctcagccgg agcgggaacg cccccgggac ggcggcggaa gaggcgaccg caggccgggc
     6541 ggcggcggaa gcggtccccg caggcactca tcgggaggaa ggccgtagcc tccaatgccg
     6601 gtggctgtaa aaattctcag ggaaggaaga gcgaaagagt tccctcttcc tgaatacgca
     6661 acggaaagcg ctgcaggcgc ggatcttcgc tcggcggagg acatgctcct cctgccgggg
     6721 gagcggacga gcgtcggcac cggcatccgg atagagcttc ccgaagggta cgaagcgcag
     6781 attcgaccca ggagcggtct ggctattcgt cacggtgtga cgatcctgaa cgctcccgga
     6841 acgatcgaca gcgattacag gggggagatc aggatcctcc tggtgaacct cgggaaagac
     6901 cctttcacca tcgtcgcggg cgacagaatc gcgcagcttg tcgtcgctcc cgtcgccaga
     6961 gttgaatggc gcgaggatga aacgctcggg ctgacgggac ggggagaggg cggtttcggg
     7021 agcaccggaa agagttgaca gattcccctt ttaggggtat aagttcagaa aagttttctt
     7081 tcctgcgatg agagggagag tagggacgat tcgtccggac agagagggag gcacgcggct
     7141 gaaaggcctc ctccggattc gtcatcgaac accgcccttg agctgacgcc gaaatacccc
     7201 tcgggacaag gcgtctgggg atccgccccg aacagcggac acgagcgccg cgcaaaagca
     7261 gttcgcgcgg aaatggagtg gaaccgcgac atgcccacgt cgtctccata atggagatgc
     7321 gtgggcattt ttttatcgaa aacgaggagg taggagtatg atgcagttta cagggaacaa
     7381 cggcaaacag gcggagttgg cttccggcac ggcgggcgat gtgctcgacg cgttggggat
     7441 gaagaagggc gcgatagcgg cgaagatcga cggctgtgcg gtggatctct cgcgcgtcgt
     7501 cgaaaggggc ggcgaggctg ccccgatact tccggagtcg gacgaggggc tcgacattct
     7561 gcggcactcc gcagcgcatt tgatggcgca ggccgtcgcc cagatgtacc cgggaacgcg
     7621 ctacggcgtc ggcccggcga tcaaggacgg tttttattac gacatggaga tccccggagg
     7681 catcaccgag gaggatctgc cgaagatcga aaaggagatg caccggatcg ccaagcgctc
     7741 cgttcccgtg gaacggcggg tgctgagcaa agccgaggcg cttgaatggt tcaaggcgaa
     7801 gaacgacccg tacaaggtag agatcatctc cgagctggaa gatgagacgg tcacgctcta
     7861 cgttcagggt gaatacgcgg atctctgccg ggggccgcac gtccccaata cctcctatgt
     7921 ccgtcacttc aagctgctct ccgtggcggg ggcgtactgg cggggcgacg agaagaacat
     7981 catgctcacc agagtctacg gaacggcctt cggcgatgag gagtccttgg cggcatacat
     8041 ccacaggatg gaggaggcga agaaccgcga ccacagacgt ctcggcaagg agctcgacct
     8101 cttcagcctc caggccgagg ggcccggttt cccgttcttt caccccaagg ggatggtcat
     8161 tctcaataac ctgatggact tctggaagaa ggagcataca aagagagggt actgcgagat
     8221 ccgcaccccg cttatcctcg aacgctcgct ctggattcag tccggccact gggatcatta
     8281 cagagacaac atgtacttta ccgagatcga cgagcgcccc ttcgctatca agccgatgaa
     8341 ctgccccggc ggcatgctcg tctacaagag ccagattcgc agctaccgcg atctgccgct
     8401 cagg
//
LOCUS       DAOPKU010000054         8363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9047_length_8363_cov_4.732186, whole genome shotgun sequence.
ACCESSION   DAOPKU010000054 DAOPKU010000000
VERSION     DAOPKU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9047_length_8363_cov_4.732186"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PK849_02760"
     CDS             <1..1004
                     /locus_tag="PK849_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPE65076.1"
                     /translation="AELLAEGQEDCLARIEEAASGVSLPLSLKTAAGSRIGIDCSVAA
                     LGEEMLVFGMYSDQENDRIITEMSVLNLEMANMTRELHKKNLQLAVSQQALAKADSMK
                     KTFLAIIAHDLRGHVGGVSQLLSMLQQDYDDMDDAEKKECVTLASDEARRTFLLLEDL
                     LSWSRSQLDQIEFTPVDCNLRALIEENIALLQSRAKEKGVALSHETPPSLSATLDRSM
                     MGTVLRNLLNNALKFTAPGGRVLVSASQKGESVEIIVRDTGTGMSQEQLENLFRIDAK
                     PVSTQGTSGEKGSGLGLILCKEFVEKNGGMIRAESVAGEGSSFFISLPSARGASDGA"
     gene            complement(1117..3246)
                     /locus_tag="PK849_02765"
     CDS             complement(1117..3246)
                     /locus_tag="PK849_02765"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPE65077.1"
                     /translation="MSVSRSELRLSLEGLNCAQCSARIEESVRLLPGVSFAALDLVAG
                     RLRVVLSGEHNGDETLSRIRGIVDSIEPGVSVSEEGAQAKSVSLPLKEIARLSAGVLL
                     WVAAMFAEVSEGVRMALYVAAYLAAGINVLRTVFGNLRQGRIFDEFFLMTIATGGAFA
                     IGEFSEAVAVMLFYESGELVQDLAVAKSRRSILSLMDIRPDSAVLLEEGGERRVRAED
                     VRVGETVLVKPGERIPLDGSVKTGTSSLDTSSLTGESVPRDVAPGDDVLAGSVNLAGT
                     LTVVVSRLFGESTLSKGLRLIEEARERKAPTERFITAFAKRYTPAVVAAAALLAVVPP
                     LMGLGAFREWGYRALIFLVVSCPCALVISVPLALFGGIGAASRRGVLLKGGNVLETLA
                     KVRTVLFDKTGTLTRGVFRVTGVFQAGNTDRERLLSIAGEAERGSNHPVARAVVEAAG
                     ALPPLGGAIEELPGRGVRLVGGGKVILAGNARLLTDSGIASAPEAEGRPGAVVHVAED
                     GRYAGYIVVEDTVRSQSKRAVERLRSLGVSTVGMLSGDRRENASRIAEDLGLDLWESD
                     LLPSDKLEHFQRISERTGGTTLFVGDGMNDAPLLAAADVGMAMGGLGADAAIEAADGV
                     ILNDDPLGAADVIEIARKTRSIVWQNIAFALGVKGAVLTLGAFGMATMWEAVFADVGV
                     ALLATLNAARVLRFGERMQREAEEIKV"
     gene            complement(3243..3629)
                     /locus_tag="PK849_02770"
     CDS             complement(3243..3629)
                     /locus_tag="PK849_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPE65078.1"
                     /translation="MNSQTSVSSDEALRLTPDEVSSRMPDERTLYELADLFRVFGDST
                     RIKILCALSVSDLCVADIASLLSMTQSAISHQLRVLKQVRLVRVARKGRSSVYSLDDD
                     HVRTIFDQGFRHVRHIAPSSERGEAK"
     gene            complement(3819..5063)
                     /locus_tag="PK849_02775"
     CDS             complement(3819..5063)
                     /locus_tag="PK849_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HPE65079.1"
                     /translation="MRHSLRILSVSFLLLFLTSSSLFGAGFALYDFSARGNALGGAMV
                     GRADDPSAIAFNPAGITQIPGSAFMTGLGFLLPSGTAVATGTAMSVDQNDRTFILPNM
                     YYTKQMNEKLWLGVGVMTRFGLGTEFPHDWFGRYNSYRAMLESVSINPNIAWKVNDAL
                     SLSVGVEALWFEADLRRKIDSTQGVAPNPATDIDLRLKGDDIGYGWNVGLHYKMDETT
                     RFGLHYRSRVKLALSGTAIATSPLATDSTEGRVDLTLPDMLMFGVSKQLTPKLNVEVG
                     AIHTGWDSYDSIAINFGKPLLGALPIRSFTPKNWASVWRYQLGLEYAYSPEWTWRLGY
                     TYDQEPMPDATIDYQAPSGDRQLFSVGFGYGKDNWALDFAYSHLISAERNIAGRRAEG
                     ILDSRTHDMNVNIFMLSYSIRF"
     gene            5395..5658
                     /locus_tag="PK849_02780"
     CDS             5395..5658
                     /locus_tag="PK849_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HPE65080.1"
                     /translation="MDGAKALAAKVLTDEELAKKLGEAKSEAEFNAVVAKLGYKCTAA
                     EFEAAFKEAQGKAPLSDEQLDQASGGLSIVGVDYAFVAVQTAK"
     gene            5743..7494
                     /locus_tag="PK849_02785"
     CDS             5743..7494
                     /locus_tag="PK849_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE65081.1"
                     /translation="MDLLLIKSHRSVCGYIPGSNLGMNILIQLLRQKKYTAEMFQGYA
                     KEALSHVKMLMEQEDRPKVIGFYCDFDNVHWVRLFAREVKRNYPEVILLAGGPQTVDM
                     DADFLKDTGILAVCIGEGEETVPELMEYCIHRRGSLETIQGIAFLDASSQLVKTPPRT
                     PPEALDSIPWPDITLTNDYKHYSLLPILTGRGCPFGCTFCYEGANAKRVRRHSVPSLL
                     HEIRANFERNPSIKYINFLDDTFTLDHKRVDEICEGIKELRKEYDFVWFASAHISLIG
                     KHRDMARTMAEAGLKKIFFGLESGSDAVLQSYNKKITRKMAVEVIAHCVDSGIHAISG
                     NIILGGPHETRETVQESEDLIMELLYRAPGQFETAYFSFLPFPQTPITLNPQKFGMEI
                     YESLIDCCNEDIPLSGTEALDFIELTKIRLQANKRLQNEMRTIYLDGKIPESVILDAY
                     RLGERYGVFTRWNDYVYKNLPIDDAYWRMRAFGEYVTGSQLGAWEENAIPHRTFELWL
                     YTDISGAKPRVVDTPLEEIDYTLLKLCSGKLSKREVIRQGKAALDPAGTQPDFYKRAE
                     EALHSMEQKKWILYQKP"
     gene            7557..>8363
                     /locus_tag="PK849_02790"
     CDS             7557..>8363
                     /locus_tag="PK849_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPE65082.1"
                     /translation="MIGNLGILTLAAQLNADGFEAYAYTGITTDVVKTIGQMKERLFA
                     VCFYCDFDNQSAVEAIIKGLKNEAGNNFYVVLGGPHTLHMTERDLERYGADAIIKGEG
                     EESLPAWLRARHTGATVAVPGEMLPGEPSEYVYLKDFSHYAPPQDEAIVGYGERPLMS
                     VITARGCPYRCAFCFEGGNSKNLRMRTPDDVLCEIEARLQKSRGPRYLFFCDDTFTAN
                     PERLSELLKGVRRLRERHDFVWFCEGHPGFLARRPELIAEMIASGMVRMQI"
BASE COUNT         1948 a         2456 c         2321 g         1638 t
ORIGIN      
        1 tggcggaact cctcgcggaa ggacaggagg actgccttgc gcgcatcgaa gaagctgctt
       61 ccggcgtgag cctgccgctc tccctgaaga cggcggcagg atcgcgcatc ggaatcgact
      121 gcagcgtcgc cgcgcttggt gaggagatgc tcgtctttgg tatgtactcg gatcaggaga
      181 acgaccggat cattacagag atgtcggtct tgaacctgga gatggccaac atgaccaggg
      241 aactccacaa gaaaaacctt cagctcgctg tctcccagca ggcgctggca aaggccgatt
      301 cgatgaaaaa aacgtttctt gccatcatcg cccacgacct cagaggtcat gtgggaggcg
      361 taagtcaact gctctcgatg cttcagcagg actatgacga catggacgac gcggaaaaga
      421 aggagtgtgt tacgcttgcc tccgacgaag cccgcagaac gttccttctt ctcgaagacc
      481 tcctctcctg gtcgagatca cagcttgacc agattgaatt cacccccgtc gactgcaacc
      541 tgcgcgcgct gatcgaagag aacatcgccc tgctgcaatc ccgcgcgaag gagaagggag
      601 tcgccctctc tcacgagacg ccgccttccc tttccgccac gctggataga agcatgatgg
      661 gaacggtgct ccgcaatctg ctgaacaacg cgctgaagtt caccgccccc ggagggcgcg
      721 tcctcgtttc cgcatcccag aagggagaga gcgtcgagat catcgtacgc gataccggaa
      781 ccggcatgtc gcaagagcag ctagagaacc tcttccgcat cgacgccaaa cccgtcagca
      841 cacaagggac gtcgggggag aaagggagcg gactcggtct tattctctgc aaggagttcg
      901 tcgaaaaaaa cggaggcatg attcgtgcgg agagcgtcgc cggagagggg agctcctttt
      961 tcatttcgct gccatccgca cgcggagcat cggacggagc gtaataataa aacacctaca
     1021 caacacgcaa caacaaagag gccgcagcaa acccccagtt cgggacgcgc tgcggcctct
     1081 tttctccact cttttggtca gtattcatct acctcttcat actttaattt cctctgcttc
     1141 gcgctgcatg cgctctccaa agcgcagaac ccgcgctgcg ttgagcgttg caagaagcgc
     1201 gacacccacg tcggcaaata cggcctccca catggtcgcc attccgaacg ctcccagcgt
     1261 gagtacagct cccttcaccc cgagtgcaaa ggcaatattc tgccagacga tgctccgcgt
     1321 cttcctggct atttcgatga cgtcggccgc gccgagcgga tcgtcgttca ggatgacgcc
     1381 gtcggcggcc tcgatcgcgg cgtccgctcc caggccgccc atggccatgc ccacatccgc
     1441 ggcggcgagc agcggagcgt cgttcattcc atcccccaca aagagcgtcg tgcctcccgt
     1501 tcgctcgctg atccgctgga agtgttccag cttatcggac ggaagaagat cgctctccca
     1561 gagatccagt ccgagatctt ccgctatgcg ggaggcgttc tcccgacggt cgcccgagag
     1621 catcccgaca gtagacaccc cgagggaacg aaggcgctcc accgctcttt tcgactgcga
     1681 gcgcaccgta tcctcgacga cgatgtaccc tgcgtatcgg ccgtcctcgg cgacatgcac
     1741 cacggccccc gggcgccctt cagcctccgg agccgaggcg atgccggagt cggtcaacag
     1801 gcgagcgttt cccgcgagaa ttacctttcc cccgccgaca agccgaacgc ccctgccggg
     1861 gagctcctcg atcgccccgc cgagaggagg aagcgctccg gcagcctcta caacggcccg
     1921 cgcaaccggg tgattcgacc ctctctccgc ctccccggcg atcgaaagca atctctcccg
     1981 gtccgtattc ccggcctgga agactccggt caccctgaac acgcccctag tcaacgtacc
     2041 cgtcttgtcg aaaagcacgg tgcggacttt cgccagcgtc tccaacacat ttccaccttt
     2101 caaaagcacg cctcgccttg aagcggctcc gataccgccg aagagcgcga gcggcacgga
     2161 gatcacaagg gcgcacggac aggagacgac gaggaagatg agcgcgcggt acccccattc
     2221 ccggaacgcg cccaacccca tcagcggcgg tacgacggcg agcagcgcgg cggcggcaac
     2281 caccgccggg gtatatcttt ttgcaaaggc ggtaatgaag cgctctgtgg gcgcctttcg
     2341 ttcccttgcc tcttcaataa ggcgcaaccc tttcgagagc gtggactcgc cgaagagacg
     2401 gctcacaacg accgtgagcg ttccggccag gttgacgctt ccggcaagca cgtcgtcgcc
     2461 gggcgcaaca tcccgcggaa cggattcgcc cgtcaacgac gacgtatcca gcgatgatgt
     2521 ccccgttttc actgaaccgt cgagaggaat tcgttcgcct ggcttcacaa ggacggtctc
     2581 ccccacccgg acgtcctccg cacgcacgcg ccgctctccc ccttcttcga gaagcactgc
     2641 cgaatcggga cggatatcca tcagcgagag aatggaacgt cgggacttcg ccaccgccaa
     2701 atcctgcaca agttcccccg actcataaaa gagcatcacc gccactgcct cggaaaactc
     2761 gccgatggcg aacgctcccc ctgtggcaat cgtcatcagg aagaactcgt caaagattct
     2821 cccctgccgc agattgccga acacagtacg gagcacgttg atgcccgccg caaggtaggc
     2881 agccacatag agtgccatcc gcaccccttc ggaaacctcc gcaaacatcg ccgcaaccca
     2941 gagcagcacc cctgccgaaa gacgcgcgat ttccttcagc ggaagagaga cgcttttcgc
     3001 ttgcgccccc tcttccgaga cggagactcc cggttctatc gaatctacaa ttccgcggat
     3061 gcgcgataag gtttcgtcac cgttgtgctc accggacaag accactctga gccgcccggc
     3121 gaccagatca agcgctgcga acgacacgcc gggaagcagg cgaacggact cctctatcct
     3181 cgcggaacat tgggcgcagt tcagcccctc cagcgagaga cgcaattcgc ttcgtgaaac
     3241 gctcacttcg cctcccccct ttcagaggag ggcgcaatgt gccgcacatg ccggaatccc
     3301 tggtcgaaga tggtccgcac gtggtcgtcg tcaaggctgt acacggacga tcgtcctttc
     3361 ctggcgacgc ggacaagacg cacctgttta agcacccgaa gctggtgcga aattgcagat
     3421 tgggtcatgg aaagcaggga cgcgatatcc gcgacgcaga gatcggaaac ggaaagcgcg
     3481 cagagaattt ttatcctggt actgtcgccg aacacccgga agagatctgc aagctcgtag
     3541 agcgtccttt cgtcgggcat ccgggaggat acttcatccg gggtcaggcg aagcgcctca
     3601 tcagacgaaa cagatgtttg agagttcatg ggggcctcct tatatgttta tatgaatata
     3661 tgagcaacta ttcatatatc ataaaaagac tctacccgca agagggagga gaaaagagca
     3721 aaaagacaga atacacaagc atgggaagaa tgtaggaggc gagacaaaaa acatcccgga
     3781 gagcgacgaa gcgggcccgc gccctccggg aatagaggtt aaaacctgat gctgtagctc
     3841 agcatgaaga tattgacgtt catgtcatgt gtccgcgagt caagaatccc ctcggcacgg
     3901 cgtccggcaa tgttccgctc ggcggagatc aggtgcgaat aagcaaagtc cagcgcccag
     3961 ttatccttcc cgtagccgaa gccgaccgag aaaagctgtc tgtcgcccga aggggcctga
     4021 tagtcgatgg tcgcatccgg catcggttcc tggtcgtacg tgtacccaag cctccaggtc
     4081 cactcgggag aatacgcata ctcgagcccc agctggtatc tccagacact ggcccagttc
     4141 ttcggcgtga aagagcggat gggcagcgcc ccgagcaagg gtttcccgaa gttgatcgcg
     4201 atggagtcgt agctgtccca cccggtatgg atggcgccta cctccacgtt gagcttgggg
     4261 gtcagctgct tgctgacgcc gaacatcaac atatctggaa gggtaaggtc cacgcggcct
     4321 tccgtcgagt cggtggccaa cggactcgtt gctatggcgg ttcccgacag ggcgagcttc
     4381 accctgctcc ggtagtgaag gccgaaccgc gtggtctcgt ccattttgta gtgcagtccc
     4441 acgttccatc cgtagccgat gtcgtcccct ttcaagcgaa ggtctatgtc cgtcgccgga
     4501 ttgggggcaa cgccctgtgt cgagtcgatt ttccgccgca gatccgcctc gaaccagaga
     4561 gcttccaccc ccaccgagag cgagagtgca tcgttgacct tccaggcgat gttcgggttg
     4621 atcgagaccg attcaagcat cgcccggtag ctgttgtagc gtccgaacca gtcgtgaggg
     4681 aactccgtcc cgagcccgaa tcgtgtcatc acgccgacgc ccagccagag cttttcgttc
     4741 atctgttttg tgtagtacat gttcggcagt ataaaagttc tgtcgttctg gtccacgctc
     4801 atcgcggttc ccgttgcgac agccgttccg ctgggcagca aaaacccaag accggtcatg
     4861 aaggcgcttc cggggatctg cgtgataccg gccgggttga acgcgattgc cgaaggatcg
     4921 tccgcccggc ccaccatagc cccgccaagc gcgttgccgc gcgcgctgaa gtcatacagc
     4981 gcgaatcccg ccccgaagag cgacgaactg gtaagaaaaa gaagaagaaa cgaaacgctg
     5041 aggatacgaa gagaatgtcg caacctatac cactcctttt gtcaggagat tatgtttgca
     5101 gaaaactcct tgcggaaaaa agtcgttcgc attatatcac gcggaagaga tttttgttac
     5161 ctgtcggtga catttgaaac aacttgtctc aagaggagtt cattcaatgg aacagactgt
     5221 atcgccgcca caacgacgcc gctcttccgt gtttcgggcg catcgcaact tctgacaagg
     5281 tctccccaaa gagctcattt gacaatatcc cggtgggctg tataataatt tctatatttt
     5341 catacgaggt gcgtatcacc taccttatag acacacaagg aggagaagaa aatcatggac
     5401 ggcgcgaaag cattggctgc aaaggttcta acggacgagg agctggcgaa gaaactagga
     5461 gaggcgaaat ccgaagcgga attcaatgcg gtggttgcaa aactggggta caagtgtact
     5521 gcggcggaat tcgaggctgc cttcaaggaa gcccagggga aggcgccgct cagcgacgag
     5581 cagttggatc aggcttcagg cggtttgagc atagtcggcg tggattacgc gtttgtagct
     5641 gtgcaaacag caaaataaac gaaggagccc cttctccttc acggatgatg aaagaggagg
     5701 ccggaatttt tccggtctcc tcttttaggc ggaggaaacc acgtggatct gttgctcata
     5761 aaatcacacc gctctgtctg cgggtacatt cccggaagca atctgggaat gaatatttta
     5821 atacagcttt tacggcaaaa aaaatataca gccgagatgt ttcagggcta tgccaaagaa
     5881 gccttgagcc atgtaaaaat gctcatggag caggaagacc ggcccaaagt catcggattc
     5941 tattgcgatt ttgataatgt ccactgggtc aggctcttcg cccgggaagt gaaaagaaac
     6001 tacccggaag tgattctgct ggcaggcggg ccgcaaaccg tcgacatgga cgccgatttt
     6061 ttgaaagaca cgggaatcct tgccgtctgc atcggcgaag gggaagaaac agttccggag
     6121 ctcatggagt attgtatcca tcgccgaggg tcactggaga caattcaggg aatagctttt
     6181 ctggatgctt cttcacagct tgtgaaaaca cccccgagaa ccccgcctga agccttggat
     6241 tccattcctt ggcctgatat caccttgacc aacgactata aacactactc gcttctcccc
     6301 attttaaccg gacggggctg tcctttcgga tgcaccttct gctatgaggg ggccaatgca
     6361 aaacgggtgc gccgccactc cgttccctcg ctgcttcacg agatcagggc aaactttgaa
     6421 cggaacccct ccattaagta catcaatttt ctcgacgaca cctttacgct ggatcataaa
     6481 cgggtcgatg aaatctgcga gggaatcaag gaactccgca aagagtacga tttcgtatgg
     6541 tttgccagcg ctcatatctc cctcatcggc aaacaccggg acatggcccg cactatggcg
     6601 gaggcggggc tgaaaaaaat ttttttcggc ctggagtccg gcagcgacgc cgttttgcaa
     6661 agctataaca agaaaattac gcggaagatg gcggtggagg tcatcgccca ctgcgtcgac
     6721 tcggggattc acgctatttc cggcaacatt attctgggag ggcctcacga aaccagggaa
     6781 accgttcagg agtccgaaga tctgatcatg gagctgctgt atagggcgcc cgggcaattt
     6841 gaaaccgcct acttctcctt tcttccgttt cctcaaacgc cgatcaccct gaatcctcaa
     6901 aaattcggga tggaaattta cgagtccctc atcgactgct gcaacgaaga tattccgctt
     6961 tccggaacgg aagcattgga tttcatagag cttacaaaga tacgccttca ggccaacaag
     7021 aggctccaga atgaaatgcg aacaatctac cttgacggca aaattcccga atcggtgatt
     7081 ctggacgcat accgtctggg ggagcgctac ggcgtattta cccgatggaa cgactatgta
     7141 tacaaaaacc tgcccatcga cgacgcctac tggagaatgc gggcgttcgg cgagtatgtc
     7201 acaggcagtc aactgggcgc ttgggaagag aacgccattc cgcaccggac cttcgagttg
     7261 tggctgtaca ccgacatcag cggggcaaaa ccgcgggttg tcgatacgcc gctggaagaa
     7321 atcgactaca cgctcctcaa attatgcagc gggaagctct cgaaacggga agtcattcgg
     7381 caggggaaag cggcgctcga ccccgcggga acgcagcccg acttttacaa acgggcggaa
     7441 gaagcgctgc attcgatgga acagaagaaa tggattctct accagaaacc atgaacgcgt
     7501 ctccccttgt tctcctcgtg tacacccaac ggctggtccc ggggaggacc tttgaaatga
     7561 tcgggaatct gggaattctc acgctcgcgg cgcagctcaa cgccgacggt tttgaagcat
     7621 acgcctatac cggcatcaca accgatgtgg tcaaaaccat tggacaaatg aaagagcgcc
     7681 ttttcgcggt ctgtttctac tgcgacttcg acaaccagtc cgcagtggag gcgatcatca
     7741 agggtctgaa aaatgaagcc ggaaataatt tctatgttgt cctgggcgga ccgcatacgc
     7801 tgcacatgac ggaacgggat ctggaacggt acggcgcgga cgccatcatc aagggggaag
     7861 gggaagagtc cctccccgcc tggctccggg cccgccacac gggcgcaaca gtcgccgtgc
     7921 cgggggaaat gcttcccggg gaaccttccg aatacgtgta tctgaaggat ttttctcact
     7981 acgctccgcc gcaggatgaa gcgatagtag gatacggaga acggcccctg atgtcggtga
     8041 tcacggccag gggttgtccg taccgctgcg cattctgttt cgaggggggc aattcgaaaa
     8101 acttgcgaat gcgcaccccg gacgacgtgc tgtgtgaaat cgaagcgcgg ttacagaaaa
     8161 gcagggggcc acggtatctg tttttttgcg acgacacctt caccgccaat cccgagcgcc
     8221 tgagtgagct gctgaaaggg gtgcggcgcc ttcgggaacg ccatgatttc gtctggttct
     8281 gcgaagggca cccgggtttt ttagcgcgcc gcccggagct gatagccgaa atgatcgcga
     8341 gcggtatggt ccggatgcag atc
//
LOCUS       DAOPKU010000055         8353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9063_length_8353_cov_6.179079, whole genome shotgun sequence.
ACCESSION   DAOPKU010000055 DAOPKU010000000
VERSION     DAOPKU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9063_length_8353_cov_6.179079"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PK849_02795"
     CDS             complement(<1..293)
                     /locus_tag="PK849_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HPE65083.1"
                     /translation="MKKVIRTDKAPDPIGPYSQGLVCGNRVYVSGQGPRNPETGKNPE
                     GIKEQTRQVLKNIKAILEAGGATMDQVVKVTAHLTNLDRDFQGYNEVYAEFFT"
     gene            438..1094
                     /gene="eda"
                     /locus_tag="PK849_02800"
     CDS             438..1094
                     /gene="eda"
                     /locus_tag="PK849_02800"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPE65084.1"
                     /translation="MNIRKFEVLRRISDLGVVGIIRTPDIEKGVAAAEAVFEGGIDVL
                     EVSTTFPGALDTMKEIIERGRCKGLILGAGTVADATTARLCIDAGAEFIVSHCFSEEV
                     ARMCNRSGIAYMPGVGTVTEVVRAMEHGVDVVKAFPGEVLGPKFIKAVHGPLPGAQIM
                     PIGGVAPGNLDDWFQAGAFAVGLGSALLKPAGTEGDFQAISETAERIVTQIAQIRAHL
                     "
     gene            1125..2054
                     /locus_tag="PK849_02805"
     CDS             1125..2054
                     /locus_tag="PK849_02805"
                     /inference="COORDINATES: protein motif:HMM:NF020038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE65085.1"
                     /translation="MSSHWIGVFAALVTGVAWGVVSPLGRMLALRGADMTTVVVVRTL
                     LAACILFLWLLLKNPRSLRISFKDGLVLLAYAVLAIPLTATGFLYSLKYLTVPAALII
                     HYTFPLATLLGGLWITGERPTAPQYIAALLVIAGVWLGVFSSGTADGSFSLLGVFWGL
                     LAVVGLSGQFLMGRVLLSGKRLSGVLLIFYSHLFGGLIMAALKTVFSGWGDLAAMQPS
                     DWGAIAIMALVGSVLGLIAYCYSLRYVTAATASHVCTVEIPSGIGLAALMSGEIPTLQ
                     EINGSLLIILAIFLAALPEDFLRRLRKERVVLK"
     gene            complement(2151..2729)
                     /locus_tag="PK849_02810"
     CDS             complement(2151..2729)
                     /locus_tag="PK849_02810"
                     /inference="COORDINATES: protein motif:HMM:NF036878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPE65086.1"
                     /translation="MRKVVYIDYENLPGVHIEDVEGTKFLIFIGENQKKIPTDTIVRM
                     QPLGERVEWISISGVGRNALDFHIAYYLAKYHTNNTVTHYILSKDAGYDPLIKHVVKF
                     GQRVKRIITLEEIKEKPVLAPELAQKYAKVLEILKKQDKTRRPKIRKTLASSIETLFQ
                     GQVSKEDTDLLIEHLFRERFIEEKNKRIAYLD"
     gene            complement(2726..3553)
                     /locus_tag="PK849_02815"
     CDS             complement(2726..3553)
                     /locus_tag="PK849_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPE65087.1"
                     /translation="MAMLALAAMLTLSDVELYYEVHGDGPPLLLIAGMASDSQSWGSS
                     APSLAEHFTVICPDNRGTGRSKPADAPVSIERMADDCAALVRSLGYEKVHVLGHSMGG
                     FVAQTLAIRFPELVDRLVLAGTGSKFSARNAALFSDALETLERGADPVLWFRNFFYWL
                     FTPAFFEQPGMLDAAVNYALNYPWPQETVSFRNQVEAVRAFDVADDMPKITAPTLVLA
                     GAEDLLFTPGECREFAARLPDATLEIIEGAAHSIHMERGNEFVRAIIEFLTDQRGRR"
     gene            complement(3716..7312)
                     /locus_tag="PK849_02820"
     CDS             complement(3716..7312)
                     /locus_tag="PK849_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HPE65088.1"
                     /translation="MPSLCSLKKTLFRSSAPLIAGVVSLLFILGCVSRADAGQQKKNI
                     LVLHSFHQGYEWTDSVQQGILDEMAPRRKDVSLSVEYLDSIRSQEASQLEAARELFRR
                     RYAHGKTLFDVILCSDDEALGFLLEYGGELFGDVPVVFCGVNNFSQERLGGRKNITGV
                     NETISVRETIEIALKLRPSAKTVAVVAGFRPAEIRNLEMAKAAEPFFKGRVAFEYLHG
                     FEPEEIANRLKELSSEDIVLYLSYLLSPSGKRLSVEENRTFVVGATDAPVFSCWDTLL
                     SYGFVGGKVVHGRSQGEIAASLALQILDGKRAEDIPVVFDSPNRYAFNASALKTFGVP
                     ESLLPPYSFLLQTTGDGLAATWDELVVRRFFSEELFSRHGAPMLIIDPESGLILAGNQ
                     AAHSFYGYPELQGMTIGSINALSSQQVQDEMQKATRLRKNHFDFRHRLADGSVRDVRV
                     YSYPIELDGREVLFSMVIDETERTIAEASLHRKERSFITVLSVAVLLQLAALFLLARS
                     AARRKKAEKELAEAYENQSILLDNIPTQIWLLTDDHTYGAVNRAHADFNGMRAEEMSF
                     RDMYEFLPGEVVEVCRRSNAEVFADARPVRTDEKIRDASGEERLLSIWKTPRLRADGS
                     VEYVICAGEDVTEQKRTEDSLRAALRELEQKTELANEMAERAQAANVAKSAFLANMSH
                     EIRTPMNGILGMTGLLLETGLTQEQRGFAGSLMSSAESLLSLLNDILDFSKIEAGKLE
                     LETLDFNLETLLKDFTSVMAVVARKKGLELRCVIEPDVPALFRGDPGRLRQILNNLTS
                     NAVKFTERGEVTTTVSLVGQDARFASLRFTVRDTGIGISPETMELLFEKFSQGDASIS
                     AKYGGTGLGLVISKQLVALMGGEIGVRSEEGKGSEFWCTVVLEKLSEAEFSAAGARGA
                     TEEPLSGFERRGRRILLAEDNITNQRVALGMLKKIGVTADAAENGEAALEALASCSYD
                     LVLMDCRMPVMDGYEATRRIRRMEGDVRNIPVIALTAYAMPGDREKCLEAGMNDYLAK
                     PIIRDALLAVLERWLPRREKEEGPFEDVATEGAREMEAQYLESVWDRDVLLERLLGDE
                     EMLEEIITGFLEDAPAQLSALSEALATGDAASAEMSAHAIKGAAAGIGAERVRSAAFA
                     VENAVRNGAMDEAASLIGALELEFGRLKRFIEERTRPNEVGDVSPENPVAKE"
     gene            7590..>8353
                     /locus_tag="PK849_02825"
     CDS             7590..>8353
                     /locus_tag="PK849_02825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE65089.1"
                     /translation="MRRDYLRMLDTLKEGVYFVDLDRKITFWNKGAERITGFRREEVV
                     GSSCMDNILVHVDCKGTQLCKEGCPLLYTMKDGSSREAEVFLHHRRGHRVAISVRTTP
                     LEDDEGNIIGGIELFSELNPEESLRQRMEELERCAMIDPLTGVPNRSYLNPELERLFR
                     LWEANRIPFGLLFFDIDHFKRVNDTYGHDVGDQVLRTVAQSLSSSVRNYDSVGRWGGE
                     EFVGFFPNANPEVLLPIAEKLRQVVRNSWIEIEGNSS"
BASE COUNT         1692 a         2573 c         2344 g         1744 t
ORIGIN      
        1 tcggtaaaaa actccgcgta cacttcgtta tacccttgaa aatcgcggtc gaggttcgtc
       61 agatgcgccg tcaccttgac cacctggtcc atcgtcgcgc cgcccgcctc caggattgct
      121 ttgatgttct taagcacttg ccgcgtctgt tccttaattc cttctggatt cttccccgtc
      181 tcgggatttc tagggccctg tcccgaaacg tagacgcggt tgccgcagac cagcccctgg
      241 gagtacggcc cgatgggatc cggcgctttg tcagtacgga taaccttttt cactgcaatc
      301 atcctctcgt aagtttttct cccccccttc cgcgaaaaaa acgccggaca tgcaaggaaa
      361 gagactttct ttgctatcat tgtatccgaa aaagctctcg aacgggaagc catactcttg
      421 tcaggaggat gcaaagaatg aatatccgaa agtttgaagt gctgcggcgg atctccgacc
      481 tcggcgtggt agggatcatc aggactccgg acatcgagaa gggcgtggcg gctgcggagg
      541 cggttttcga aggaggcatc gacgtgctcg aagtctccac gaccttcccg ggcgccctcg
      601 ataccatgaa ggagatcatc gaacgcggcc gctgcaaagg gttgatactc ggcgcgggaa
      661 cagtcgccga cgcaacgacg gcgcgcctct gcatcgacgc gggggcggag ttcatcgtct
      721 ctcactgctt cagcgaggag gttgcgcgga tgtgcaacag gtccggcatc gcctacatgc
      781 ccggcgtggg gacggtcacc gaggtcgtcc gcgcgatgga gcacggcgtc gacgtcgtga
      841 aggcgtttcc gggagaagtg ctggggccga agttcatcaa ggcggtccac ggtcccctgc
      901 ccggcgcgca gatcatgccg atcggcggcg tggcgccggg caatctcgac gactggttcc
      961 aggcgggcgc cttcgccgta gggctcggaa gcgcgctcct gaaaccggcg ggaacggaag
     1021 gagacttcca agccatcagc gagacagccg aacggatcgt cacccagatt gcccagatcc
     1081 gcgctcatct ataaggcgaa gtccgccgca ggagggaaga gaggatgtca tcacactgga
     1141 taggcgtttt cgcggcgctg gtcaccggcg tcgcatgggg ggtcgtcagc ccgctcggcc
     1201 gcatgctcgc ccttcggggc gccgacatga cgaccgtggt cgtcgtacgg acgcttctgg
     1261 cggcatgcat tctctttctc tggctcctgc tgaaaaatcc ccggtctctc cgcatttctt
     1321 tcaaggacgg attggtgctt cttgcgtacg ccgtcctcgc cattccactc acggcgaccg
     1381 gatttttgta ttctctgaag taccttacgg ttccggcggc gctcatcatc cactacacat
     1441 tccccctcgc cacgctgctc ggagggctct ggatcaccgg ggaacggccg accgccccgc
     1501 agtacatcgc cgcgctcctt gtcatcgcgg gcgtctggct cggcgtcttc tcatccggta
     1561 cggccgacgg gagcttttcg cttctcgggg tcttctgggg gttgctcgcg gtcgtcgggc
     1621 tttcgggcca gtttctgatg gggcgggtgt tgctctcggg caaacgcctc tccggtgtgc
     1681 tcctgatctt ctactcccac ctgttcggcg gtctgatcat ggccgcgctc aagactgttt
     1741 tctcggggtg gggcgacctg gcagcgatgc agccctcgga ctggggagcg atcgcgatca
     1801 tggcgctcgt cggcagcgtg ctcgggctca tcgcgtactg ctattccctg cgctacgtca
     1861 ctgcagcgac ggcgagccac gtctgcaccg tggaaattcc ctcggggatc ggccttgccg
     1921 ctctcatgag cggtgaaatc ccaacgctcc aggagatcaa cggcagtctg ctgatcatcc
     1981 ttgcgatctt cctcgccgcc cttcccgagg acttcctgcg cagactgcgg aaggaacgtg
     2041 tcgtcctgaa gtaggcggga atccgttctt tctaaaaagt aacccaaaat aaaaggaagg
     2101 aaccggcttc gggcggttcc ttccttttat tttgcgcttt gaaaaaactc tcagtccaga
     2161 taggcgatgc gcttgttctt ctcctcgatg aaccgctcgc ggaacaagtg ctcgataagg
     2221 aggtcggtat cctccttcga aacctgcccc tgaaagagcg tttcgatgct cgacgccagc
     2281 gtcttgcgta tcttcggacg gcgcgtcttg tcctgtttct tcagtatttc caacactttg
     2341 gcgtacttct gcgccagctc cggggcgagg acgggcttct ccttgatctc ctcgagcgtg
     2401 atgatgcgct tgaccctctg gccgaacttg acgacgtgct tgatcagcgg gtcgtacccc
     2461 gcgtccttcg agagaatgta gtgggtcaca gtattgttcg tgtggtactt cgccaggtag
     2521 tacgcgatat gaaagtccag cgcgttccgc cccacgccgc tgatggaaat ccactccacc
     2581 cgttcgccga gcggctgcat ccggactatg gtatcggtgg gaatcttctt ctgattctcc
     2641 ccgatgaaaa tgaggaactt cgtcccctcc acatcttcga tatggactcc cggaagattc
     2701 tcgtaatcaa tgtagacaac ctttctcatc ggcgccccct ttggtcggtt aagaattcga
     2761 tgattgcgcg tacaaactcg tttccccgct ccatgtggat ggagtgggcc gccccctcga
     2821 ttatttcgag cgtcgcatcc ggcaggcggg ctgcaaactc ccggcattcc cccggcgtga
     2881 acagaagatc ctccgcgccg gcaagcacca gggtcggcgc ggtaatcttc ggcatgtcgt
     2941 cggcgacgtc aaaagcgcgg accgcctcga cctggttccg aaaagagacc gtctcctgag
     3001 gccacggata gttcaacgca tagttcaccg cggcgtcgag cataccgggc tgctcgaaaa
     3061 acgcgggggt gaacaaccag tagaagaagt tgcggaacca gaggacggga tcggcgccgc
     3121 gttcaagcgt ttccaaagca tcggagaaaa gggccgcatt tcgtgccgag aactttgaac
     3181 ccgttccggc gagcacaagc ctgtccacaa gttcgggaaa gcgaatcgcc agtgtctgcg
     3241 cgacgaatcc ccccatcgag tgcccgagaa cgtgcacttt ttcgtatccg agggagcgga
     3301 ccagcgcggc gcagtcgtcc gccatcctct cgatcgagac cggggcgtcc gcgggcttgg
     3361 agcgtcccgt tccccggttg tcggggcaga tgacggtaaa atgctccgca agcgagggcg
     3421 ccgaagagcc ccagctttgc gagtccgacg ccatcccggc gatcagcagc agaggcggcc
     3481 cgtcgccgtg cacctcgtag tacagctcga catcgctcaa cgtgagcatt gcggccagcg
     3541 ccaacatcgc catcacaaaa cccctttcag ataaatgaag agcgggaaaa agacttccat
     3601 aacccgccgt tctccggtca ttccataagg gcacgggcgg acggcattac tataccacgc
     3661 ggcggctctt ttcagaaaga gaccaaacgc ctcccgaaag aaagcgttcc acgcgtcact
     3721 ctttcgcaac cgggttttcc ggggaaacgt ctcccacttc gttcggacgg gttctttctt
     3781 cgatgaagcg cttcaacctc ccgaactcga gctcgagcgc tccgataaga gaagcggctt
     3841 cgtccatcgc tccgttgcgt acggcattct ctaccgcgaa cgccgcgctt cggaccctct
     3901 ccgctccgat acccgccgca gctcccttga ttgcgtgcgc ggacatctcc gcggaagcgg
     3961 cgtctcccgt cgcaagagct tcggaaagcg cagaaagctg cgccggcgcg tcctccagaa
     4021 agcctgtgat gatctcttcg agcatctctt catccccgag aagacgttcc aacaggacgt
     4081 ccctgtccca gaccgactcc agatattgcg cttccatttc ccgggctcct tccgtcgcaa
     4141 cgtcctcaaa cggcccttct tccttctccc ggcgggggag ccagcgttcc agcacagcga
     4201 gcagcgcatc gcgaataatc ggcttggcga ggtagtcgtt catgcccgct tcaaggcatt
     4261 tctccctgtc tccgggcatg gcgtacgcag tcagtgcaat aacgggaata ttccggacgt
     4321 cgccctccat gcggcggatt ctgcgggttg cctcgtatcc atccatcacg ggcatccggc
     4381 aatccatcaa cacaaggtcg taagagcatg aggcaagggc ttcgagcgca gcttcgccgt
     4441 tttcggcggc atccgccgtc acgccgatct tcttcagcat gcccagggca accctctggt
     4501 tagtgatatt atcctcggcc agcaagattc gtcgtccccg ccgttcaaaa ccggacaggg
     4561 gctcttctgt cgcgccccgt gcgccggccg cagaaaactc agcctcgctc aacttctcca
     4621 ggacgaccgt acaccagaac tcggatcctt tcccctcttc gctccgtacc cctatttcgc
     4681 ctcccatcaa ggcgacaagc tgtttcgaaa ttacaagacc aagccctgtg ccgccgtact
     4741 ttgcggagat cgacgcgtct ccctggctga acttttcaaa gagcagctcc attgtttcgg
     4801 ggctgattcc tattcccgta tcccgaacgg taaaacgcag agacgcgaaa cgcgcatcct
     4861 gtccgaccag cgagacggtt gtcgtcactt cgccgcgctc cgtaaacttg acggcgttgc
     4921 tcgtaaggtt gttgagtatc tgacgcaaac ggccgggatc cccccggaac agcgccggga
     4981 cgtccggttc gatgacgcag cgcagttcca gccccttctt ccgggcaacg acggccatca
     5041 cggaggtaaa atccttcagg agcgtctcca ggttaaaatc aagcgtttcc agctccagct
     5101 ttccggcctc aatctttgag aagtcgagaa tatcgttgag aagagagagc agcgactcgg
     5161 cgctcgacat caaactcccg gcaaaccccc gctgctcctg cgtcaaaccg gtctccagca
     5221 gcaggccggt cattccaaga atgccgttca tgggcgtgcg tatctcgtgg ctcatattcg
     5281 ccagaaacgc gcttttcgca acgtttgccg cctgcgcgcg ctcggccatc tcgttcgcca
     5341 gctccgtctt ctgttcgagt tccctcagcg cagcgcgcaa cgaatcctcc gttcgctttt
     5401 gctcggtaac gtcctccccg gcgcagatga catactccac actgccgtcg gcacggagcc
     5461 gcggcgtttt ccaaatggag agaagccgct cttctccgga agcgtcgcgg atcttctcgt
     5521 cggtacggac agggcgggcg tcggcaaaga cctccgcgtt cgatctccgg cagacctcga
     5581 cgacctcgcc cggcagaaat tcgtacatgt ccctgaacga catctcctcc gcccgcatcc
     5641 cgttgaagtc ggcgtgcgca cgattcaccg cgccgtatgt gtggtcgtcg gtcagaagcc
     5701 agatctgcgt cggaatgttg tcgagaagga tactctggtt ttcgtaggct tctgcaagct
     5761 ctttctccgc tttctttctc cgcgcggcgc ttctcgcaag gagaaagagc gccgcaagct
     5821 gcaagagtac cgcaacggac aacaccgtga taaacgaacg ctccttgcga tgaagagagg
     5881 cttctgcgat cgtcctctcc gtttcgtcga tcaccatgga gaagagtacc tctctcccgt
     5941 cgagctcgat cgggtaggag taaacccgta catcgcgcac cgaaccgtcg gcaagcctgt
     6001 ggcgaaaatc gaagtgattc ttccgcagcc tggtcgcttt ttgcatctcg tcctgcacct
     6061 gctgcgaaga gagtgcattg atgctgccga ttgtcatccc ctgtagctcc gggtaaccgt
     6121 agaagctgtg cgctgcctga ttccctgcca gaatgagtcc gctctccgga tcgatgatga
     6181 gcatgggcgc cccgtgtctg gagaagagct cttcgctgaa gaagcggcgg acgacaagct
     6241 cgtcccatgt cgcggccaat ccgtccccgg ttgtttgaag aagaaacgaa tacggcggaa
     6301 gaagcgactc gggtacgccg aacgttttga gcgcggaggc gttgaaggcg taccggttgg
     6361 gactgtcgaa aacgaccggg atatcctccg ccctttttcc gtccagaatc tgcagcgcaa
     6421 gcgacgcggc gatctccccc tgagaccgcc cgtgcaccac ctttccgccg acgaaaccgt
     6481 acgaaagcag cgtatcccag cagctgaaga cgggggcgtc cgttgccccg acgacgaacg
     6541 ttctgttctc ctcgaccgag agccttttcc ctgaagggga gaggaggtag gagaggtaga
     6601 ggacgatatc ttcggatgaa agctccttca gccgatttgc gatctcctcc ggttcgaaac
     6661 cgtgcaaata ctcgaacgcc actcttccct tgaagaaagg ttccgcggcc tttgccatct
     6721 ccaggttgcg aatttcggcg gggcggaaac cggccaccac ggcgaccgtc ttcgcggaag
     6781 gacgaagctt cagggcgatc tctatcgtct ctctgacgga gatcgtttcg ttgacgcccg
     6841 taatgttctt ccttccgccg aggcgctcct gtgaaaagtt attgacgccg cagaagacga
     6901 cggggacgtc gccgaagagc tctccgccgt actccagaag aaagccaagc gcttcgtcgt
     6961 cgctgcagag gatgacgtcg aaaagcgttt tcccgtgtgc gtaccggcgg cggaagagtt
     7021 cgcgcgccgc ctccagttgg ctcgcctcct gagagcgtat ggagtccaga tactccacgg
     7081 agagcgatac gtccttccgc cggggcgcca tctcgtcgag gatgccttgc tggacggaat
     7141 cggtccactc gtacccctga tggaacgagt gcagcacgag gatatttttc ttttgttgcc
     7201 cggcgtcagc ccgcgaaacg catccaagaa tgaagagcag cgacacaacg cccgcgatca
     7261 gcggtgctga agaacgaaac agagtttttt tcaaggaaca aagagatggc aagaacacct
     7321 cttcaccagc tttcagttat aataaaagcg tcaataagcg ctatggcgtg cggcttccta
     7381 aaaagcgaat tgtcatgtac tattcatacc aagcatttgt aaatctcact atatcgcaca
     7441 aaatctttga tgtaaagaaa acatcgggaa agaacacacg aagacgctgc ctggagcaaa
     7501 cggtgaatcg gcgcaaacaa aaccagagta tccttctgta ctttttagaa ataaaattga
     7561 aaacagcctc ctacgaaagg atgaaaagaa tgcggcggga ctatcttcgg atgctggata
     7621 cgctcaagga aggagtctac ttcgtcgacc tcgacagaaa gatcaccttc tggaacaagg
     7681 gcgcggagcg aatcaccggg tttcgcagag aggaagtcgt cgggagctcg tgcatggaca
     7741 acatcctcgt gcacgtcgac tgcaagggaa cccagctttg caaagagggg tgtccgctcc
     7801 tctacaccat gaaggacggc tcctccagag aggccgaggt ttttctgcac cacaggagag
     7861 gacacagggt cgccatctcc gtgcgcacaa ccccgcttga ggacgacgag ggaaacatca
     7921 tcggcggcat cgagctcttc agcgagctca accccgagga gagcttgcgt cagcggatgg
     7981 aggagcttga acggtgcgcg atgatcgacc cgctcaccgg cgtgccgaac aggagttacc
     8041 tgaatccgga gctggaacgc ctcttccgct tatgggaggc taaccgcatc ccgttcggac
     8101 tgctcttctt cgatatcgac cacttcaagc gggtcaacga cacctacgga cacgacgtcg
     8161 gcgatcaggt gctgcgaacg gtcgcgcaat cgctctcatc ctccgtcaga aactacgaca
     8221 gcgtcggacg ctgggggggc gaggagttcg tcggcttctt cccgaacgcc aacccggagg
     8281 tgctcctccc gatcgctgaa aagcttcgcc aggtcgttcg caactcctgg atcgaaatag
     8341 aaggaaacag ctc
//
LOCUS       DAOPKU010000056         8165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9343_length_8165_cov_4.955980, whole genome shotgun sequence.
ACCESSION   DAOPKU010000056 DAOPKU010000000
VERSION     DAOPKU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9343_length_8165_cov_4.955980"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PK849_02830"
     CDS             complement(<1..531)
                     /locus_tag="PK849_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65090.1"
                     /translation="MAFQEFRIDTHVFEEINDYLTDPQNPVLADLRRVVAKYGTVRDI
                     NERAREAGKTESLVKKLERMNSPFVKDLEWLASLRDEGKFVSLSDYRARVNPERPVWD
                     YDFSKAPTLEISALQYFPWLLAEVRQTVEKGEILPGRFIRVRKMKEQEADGGDLPAVR
                     AAIKILGATCVETLDTK"
     gene            complement(911..1975)
                     /locus_tag="PK849_02835"
     CDS             complement(911..1975)
                     /locus_tag="PK849_02835"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPE65091.1"
                     /translation="MRVDSSLGLCFIGFGNVGQGLARILVRKERELAERWGFAFRTVA
                     VVTRTRGAAVSRDGEGLSLADLLARVERGDPVGNERISPVEAALLPQTDIVLDVTPTN
                     LINGEPGLSLTRAALGSGRSVVSSNKGPVSLALAELKELARARGAHYRFEGSVMSGTP
                     SLNLALESLAGCDVGEVQGIVNGTTNYILTRMEEGFEYADALREAQEKGYAEADPAGD
                     VDGWDAAVKAQILAAAVLGFPLPLDAVERTGIAAISRADVEAAAKRGKRIKLLAKAAR
                     TPDGVAASVAPAEIPLSHPLASVGGATNALTFTTDTLRDVTIIGPGAGREETGQALLS
                     DMLAIAGAERHGARFSVKAG"
     gene            complement(1966..3168)
                     /locus_tag="PK849_02840"
     CDS             complement(1966..3168)
                     /locus_tag="PK849_02840"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPE65092.1"
                     /translation="MWKEGEGVVLKFGGSSVATPERIRAAARRVASFARQGRRTAVVV
                     SAMGKTTDDLLGLARATAPSAGDRRELDRLLATGEQQSAALLAMALSAEGCPARSFSG
                     DEAGFVAEGAWGEGRILSVDPARIEAATAFGHVAVITGFQACTRDGETITLGRGGSDL
                     SAIALAAALGAAECRIFTDVDGIYTADPRVVPGAVKLDRIPWDECLEMAFCGAAVMQA
                     RSIEMAGRMSIPLCVASSFQESEGTWIMERNVDEAVFIRSVASDDDLAVLTVEGRTVE
                     RELMDALSGEGIRTTAMVGAAGASFHIEGARLPEAVEICRKLAGCSVSVNETLSRVSV
                     IGPGAGNHPEVCRAMLGILAEMGATVHLLTSSALSVTCVVDRDRSAEAVRALHEEFIG
                     KKGVFQCA"
     regulatory      3668..3839
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4141..5514
                     /locus_tag="PK849_02845"
     CDS             4141..5514
                     /locus_tag="PK849_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HPE65093.1"
                     /translation="MNLKTKLFGRVYQFKNVKEVLAKANEEKSGDRLAGIIAESMEER
                     IAAKHVLANLTVADLRNNPVIPYEQDEVTRIIQDDVNERIYSEIAQWTLSDLREWILC
                     DETSGDDIKRVSRGLTSEVVAGVAKLMTNLDLVYGASKIRIQAHCNTTIGLPGTLSAR
                     LQPNHPTDDIEGVTLSVFEGLSYGVGDAVIGLNPVDDTVDNVKKILFKFQEIKEKFEI
                     PTQMCVLAHVSTQMEAVRQGAPTDLFFQSVAGSQKGNEAFAISGDLLSEAMELAAKQG
                     TGVGPNFMYFETGQGSELSSDSHFGADQVTLEARCYGFAKRFKPFMVNTVVGFIGPEY
                     LYDSRQVIRAGLEDHFMGKLTGISMGCDACYTNHMKADQNDIENLAVLLTSAGCNYFM
                     GVPHGDDVMLNYQCTGFHEAAALRQLLRVRPVAAFEKWLEKMGIMENGILTKKAGDAS
                     IFCVRGG"
     gene            5519..6403
                     /gene="eutC"
                     /locus_tag="PK849_02850"
     CDS             5519..6403
                     /gene="eutC"
                     /locus_tag="PK849_02850"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HPE65094.1"
                     /translation="MVTELELKQMVQQILVEMSGKESVAQKVGPLPVQSASSDDGDCL
                     PDLTEIDLRKQCLVPAPADAEALMAMKLSTPARIGVWRAGPRYKTETLLRFRADHAAA
                     QDAVFSHVSDEFLAANGLETIQTECTSKDQFITRPDLGRQFSAEAKEAMKRIAGPSPK
                     VLVYVSDGLSSTAVETNAMDTLKMIGQGLERQGVKLAKPFFVKYGRVPAMDVVSEVTG
                     ADVVCVLLGERPGLVTAESMSAYIAYKATVGMPEAKRTVVSNIHAGGTPAVEAGSYIA
                     DIIKLMLEKKISGIDLKL"
     gene            6434..7087
                     /gene="eutL"
                     /locus_tag="PK849_02855"
     CDS             6434..7087
                     /gene="eutL"
                     /locus_tag="PK849_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HPE65095.1"
                     /translation="MKGDPLKATLLSVKIIPNVNPEMAASLNLSPEQRSLGIITADSD
                     DVTYTALDEATKKADVAVVYAKSFYAGAANANTKLAGEVIGILAGPNPAEVRSGLNAV
                     ADFIENDAWFVSANEENTIPYYAHCVSRTGSYLSKVAGVNEGDAIAYLIAPPLEAMYA
                     LDAALKAADVKLAAFYGPPSETNFGGGLLTGSQSACKAACDAFAQAVIFVAENPVLY"
     gene            7095..7331
                     /locus_tag="PK849_02860"
                     /pseudo
     CDS             7095..7331
                     /locus_tag="PK849_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            7721..>8165
                     /locus_tag="PK849_02865"
     CDS             7721..>8165
                     /locus_tag="PK849_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPE65096.1"
                     /translation="MTQLQDRDLLSVQEVRNLVAKAKEAQKRLASMSQGKIDELVAAV
                     AEAACRHAERLGRMAHEETGFGKPEDKTLKNLFAAKSVYESIKDLRTVGIVREDTKNR
                     MFEVAVPVGVIAGLIPSTNPTSTAIYKAMVALKAGNGIVFSPHPSA"
BASE COUNT         1498 a         2471 c         2756 g         1440 t
ORIGIN      
        1 cttggtgtcc agcgtctcga cgcaggtcgc gcccagtatc ttgatcgcgg cgcggactgc
       61 ggggaggtcg ccgccgtcgg cctcctgctc tttcatcttg cgcacgcgga tgaagcggcc
      121 cgggagaatc tccccctttt ccacagtctg ccggacttcg gcgagcagcc acgggaagta
      181 ctggagcgcg ctgatctcga gcgtcggagc cttggagaag tcgtagtccc acacggggcg
      241 ctcggggttg acgcgggcgc ggtagtcgga gagggagacg aacttcccct cgtctcggag
      301 cgaggccagc cattcgaggt ccttcacgaa gggggagttc atccgctcca gcttcttgac
      361 gagcgactcc gtctttccgg cctcgcgggc ccgttcgttg atgtcgcgta ccgtgccgta
      421 tttggcgacg accctcctga ggtcggcgag cacggggttt tgggggtccg tcagatagtc
      481 gttgatctcc tcgaaaacgt gcgtgtcgat acggaactcc tgaaatgcca tcggtgtttc
      541 ctccttataa tagtgttggg ttacaaaaaa ccgggggcct ccgaaatgtt gatttcgaag
      601 acccccggcg gaagcgtacg cagtgtaggc gccctacgct ccgggagggt cgctgcacat
      661 catcatggcg aaaaagttac agaagcgcga agcatgcgaa gaccggttca aaaaagtcat
      721 gccgccagga ttcctttcta tttcatggta tggaacattc tccacgaaaa cgccgtttct
      781 gtcaaccgtg ttccggcgca ttcgcgacgg cgcattgcgt tcgttcgccg tcgatccgca
      841 gcgtgctcgg cggagcgtgt cgcaacggcg gcgcactgtt ttcgcgctgc ttccgcgtct
      901 gcggtccgtc ttaccccgcc ttgaccgaga agcgcgcgcc gtgtcgctcg gcgccggcga
      961 tggcgagcat gtcggagagg agggcctgtc cggtctcctc gcgccccgcg ccggggccga
     1021 tgatggtgac gtcgcgcagg gtgtccgttg tgaaggtgag ggcgttcgtc gcgccgccca
     1081 cgctcgccag cgggtgggag aggggaattt ccgccggggc gacggaggcg gccacgccgt
     1141 cgggcgtacg cgcggccttc gccagcagct tgatccgctt gccgcgcttc gcggcggcct
     1201 ccacgtcggc gcgggagatg gcggcgatgc cggtgcgctc cacggcgtcg agcggcagcg
     1261 ggaagccgag gacggcggcg gcgaggatct gggccttcac ggcggcgtcc cacccgtcga
     1321 cgtctccggc cgggtcggcc tcggcgtacc ccttctcctg cgcctcgcgg agagcgtcgg
     1381 cgtactcgaa cccttcttcc atgcgggtga ggatgtagtt cgtggtgccg ttgacgatcc
     1441 cctgcacctc gcccacgtcg caccctgcga gcgattcgag ggcgaggttg agcgacgggg
     1501 tgccgctcat cacggagcct tcgaagcggt agtgcgcgcc ccgcgctcgg gcgagctcct
     1561 tcagttcggc gagggcgagg gagacggggc ccttgttcga gctgacgacg gaccgccccg
     1621 acccgagcgc ggcgcgggtg agcgacagcc ccggctcgcc gttgatcagg ttggtgggcg
     1681 tgacgtcgag gacgatgtcg gtctgtggaa ggagggccgc ctcgacgggg gagatgcgtt
     1741 cgttcccgac ggggtcgccc ctttcgacgc gggcgagcag gtccgcaaga gagagccctt
     1801 ccccgtcgcg cgagacggcc gcgcccctgg tgcgggtgac gacggcgacg gtgcggaagg
     1861 cgaagcccca gcgttccgcg agttcccgct ccttgcggac gaggatgcgg gcgagcccct
     1921 gcccgacgtt gccgaagccg atgaagcaga gtcccaaaga agagtctacg cgcattggaa
     1981 tacccctttc ttccctatga attcttcatg aagcgcccgg acggcctccg ccgagcggtc
     2041 cctgtccacg acgcaggtga cggagagcgc cgacgaggtg agcaggtgga cggtggcgcc
     2101 catctccgcc agaatgccga gcatggcgcg gcacacttcg gggtggttcc ccgcgcccgg
     2161 cccgatcacg gagacgcggg agagcgtttc gttgaccgag acgctgcatc cggccagctt
     2221 ccggcagatt tcgaccgcct cggggaggcg cgccccttcg atgtggaagg atgcgccggc
     2281 cgcgcccacc atcgcggtgg tgcggatgcc ctctccggag agcgcgtcca tcagttcgcg
     2341 ctcgaccgtt ctcccctcca cggtgagcac cgcaaggtcg tcgtcggatg cgaccgacct
     2401 gataaagaca gcttcatcca cgtttcgttc catgatccat gttccctcgc tttcctgaaa
     2461 gctggatgcg acgcacaggg ggatgctcat cctgcccgcc atttcgatgc tgcgcgcctg
     2521 catgaccgcg gcgccgcaga aggccatttc gaggcactcg tcccagggga tccggtcgag
     2581 cttgaccgcg ccggggacga cgcgcgggtc cgccgtgtag atgccgtcga cgtcggtgaa
     2641 gatgcggcat tcggccgccc cgagggccgc ggccagggcg atggcggaga ggtccgagcc
     2701 gccgcgtccg agggtgatcg tctcgccgtc ccgcgtgcac gcctgaaagc cggtgatcac
     2761 ggcgacgtga ccgaacgcgg tggccgcctc gatccgcgcc gggtcgaccg agaggatgcg
     2821 cccctctccc cacgcgccct cggcgacgaa tccggcctcg tcgccgctga acgagcgcgc
     2881 cggacacccc tcggcggaga gggccatcgc aagcagcgcg gccgactgct gctctcccgt
     2941 ggccagcagc ctgtccagct cgcgcctgtc gcctgccgac ggggcggtcg ctctcgccag
     3001 ccccagaaga tcgtcggtgg tctttcccat ggcggagacg acgaccgccg tcctgcgccc
     3061 ctgccgggcg aaggaggcga cacgccgcgc cgcggcgcga atccgttcag gcgtcgccac
     3121 ggacgagccg ccgaacttga gtactacccc ttctccttct ttccacacaa ccgccgacct
     3181 cccttatgcg tacaaaaaaa gccgggcgcc ttcatctacg aagacccccg gctgaagcta
     3241 tcgcgcacgc ggtactaagc gccgaccgct ccaggagggt cgccgctagt catcgtcatt
     3301 gcacatgcga acagaactgc cgcgcggatc tttttcgcat tggtcatagt gcggggacct
     3361 cctttcgaat cggtttaggt tgggcaggat aatacgcccc ttccggtgac ttgtcaagcg
     3421 caggaagtga aaaaaatccg cagggtggaa aaatgcgcgc ctccctttcc cctctccgtc
     3481 gctgcaagag cgggcgggga ggggaaataa cgggtggcag gagtcttgtc tgcctttaca
     3541 aggatgtcga tttttaaatt atatagaaat tatttctaaa tggaggactt ctgttgactc
     3601 tttgtgaaga accgttgaca tttttctatc catgatatac tctgattgct ggtgaaaaag
     3661 ttgctctagt ttattttttt gtcgaggtgc gcgcgatgct cgggaagccg gtaagagtcc
     3721 ggcgcgggcc cgccactgta cgcctgacga agccgcgggg ttccgccgga gtcttccggg
     3781 aaggagcggt ctgaggatga aggcaagcca ggagaccgat tgcgcgcatc gggcagaaac
     3841 gccttgcggg gacttgaggc cgtttacgga gaagaccggc ggtgaacacc ggtgtttttc
     3901 gcggatgcgc cccgggtctt tttttgtacg cgaaattccc cgggtgcggg aagcgcgcgg
     3961 cgccgtattg ctacgaaagg ggggtggttc gggagagttg cactgatgcc gtatgctctt
     4021 cgggggcggt ccgttcccgg agacgtatcg ccggttgcgc acgtccgtcc gcgttcctgg
     4081 ccgctcttcg gggctccggc gcgtgaggac gcatgaaaac acattggagg tgaaatgacg
     4141 gtgaacctca aaaccaagct ctttggtcgt gtgtaccagt ttaaaaatgt caaagaggtt
     4201 cttgccaagg cgaatgagga gaagtccggc gaccgcctcg ccggaatcat cgcggagtcg
     4261 atggaggaac ggatcgcggc gaagcacgtg ctcgccaacc tgaccgtcgc ggatctccgg
     4321 aacaatccgg tgatcccgta cgaacaggac gaagtcaccc gtatcatcca ggacgacgtg
     4381 aacgaacgga tttactccga aattgcacaa tggacgctct ccgacctgcg cgagtggatc
     4441 ctgtgcgacg aaacttccgg agacgacatc aagcgggtca gccgcggact caccagcgaa
     4501 gtggtagcgg gcgtggccaa gctgatgacc aacctcgacc tcgtctacgg agcgagcaag
     4561 atccgtatcc aggcgcactg caataccacg ataggccttc cggggacgct ttccgcccgc
     4621 ctgcagccca accaccccac cgacgatatc gaaggcgtga cgctctccgt cttcgaaggc
     4681 ctctcttacg gagtgggcga cgcggtgatc ggtctgaacc ccgtcgacga cacggtcgac
     4741 aacgtcaaga aaatcctctt caaattccag gagatcaagg aaaagtttga aattcccacc
     4801 cagatgtgcg tcctcgcgca cgtgagcacc cagatggagg ccgtccgcca gggcgctccg
     4861 accgacctct tcttccagag cgtcgccggt tcccagaagg ggaacgaggc gttcgccatc
     4921 tccggcgatc tgctgagcga ggcgatggag ctggccgcga agcagggaac aggcgtcggc
     4981 ccgaacttca tgtacttcga gacggggcag ggctccgagc tgtcgtccga ctcgcacttc
     5041 ggcgccgacc aggtgacgct ggaggcccgc tgctacggct tcgcgaagcg cttcaagccg
     5101 ttcatggtga acacggtcgt cggcttcatc ggccccgagt acctgtacga cagccggcag
     5161 gtcatccgcg cgggattgga agatcacttc atgggcaagc tgaccggcat ctccatgggg
     5221 tgcgacgcct gctacaccaa ccacatgaag gccgaccaga acgatatcga aaacctggcc
     5281 gtcctgctca cctcggcggg gtgcaactac ttcatgggag ttccccacgg cgacgacgtc
     5341 atgctgaact accagtgcac cggtttccac gaggcggcgg cgctgcgtca gctgctccgc
     5401 gtccgtccgg tcgccgcgtt cgagaagtgg cttgaaaaga tgggaatcat ggagaacgga
     5461 atcctgacga agaaggccgg agacgcgtcc atcttctgcg tgaggggagg ctgagattat
     5521 ggtaaccgag cttgaactca agcaaatggt ccagcagatt cttgtggaga tgagcgggaa
     5581 ggaaagcgtt gcccaaaagg ttgggcccct gccagtgcag agcgcttcca gtgacgatgg
     5641 agactgtctc cccgatctga cggagatcga tctgagaaaa cagtgccttg tccccgctcc
     5701 ggcggacgcc gaggcgctga tggcgatgaa gctgtcgact cctgcgcgta tcggcgtatg
     5761 gcgcgcgggg ccccgttaca agacggagac gctgctccgc ttccgggcgg accacgcggc
     5821 ggcgcaggac gcggtgttca gccacgtatc cgacgagttc ctcgcggcga acgggctgga
     5881 gacgatccag acggagtgca caagcaagga tcagttcatt acgcgccccg acctggggcg
     5941 gcagttcagc gcggaggcga aagaggcgat gaaacgtatc gccgggccgt ctcccaaggt
     6001 gctggtgtac gtttccgacg ggctcagctc gaccgcggtg gagaccaacg cgatggacac
     6061 gctgaagatg atcgggcagg gtttggagcg gcagggcgtg aagctcgcga agccgttctt
     6121 cgtgaagtac gggcgcgttc cggcgatgga cgtcgtctcc gaagtgacgg gcgcggacgt
     6181 cgtctgcgtg ctgctcggcg aacgtcccgg actggtgacc gccgagagta tgtcggccta
     6241 tatcgcctac aaggcgacgg tcggtatgcc ggaggcgaag cgcaccgtgg tgtcgaacat
     6301 ccatgccggg ggaactccgg cggtggaggc gggcagttac atcgcggaca tcatcaagct
     6361 gatgctcgag aagaagatca gcggcatcga tctgaaactg taagtatttc ttactgtcgg
     6421 gagggaagat gaaatgaagg gcgatccctt gaaggcaacg cttttgagcg tgaagatcat
     6481 ccccaatgtg aatccggaga tggcggcttc gctgaacctg agtccggagc agaggagcct
     6541 cggcatcatc accgcggaca gcgacgacgt gacctacacg gcgctggacg aggcgacgaa
     6601 gaaggcggat gtggcggtgg tgtacgcgaa gtcgttctat gcaggcgccg cgaatgcgaa
     6661 cacgaagctc gcgggagaag ttatcggcat ccttgcgggg ccgaaccccg ccgaggtgcg
     6721 gagcggactg aacgccgtcg cggacttcat cgaaaacgac gcgtggtttg tttcggcgaa
     6781 cgaggagaat acgattccgt actatgcgca ctgcgtctcc cgcacgggaa gctatctttc
     6841 caaggtggcg ggagtgaacg agggcgacgc gatcgcctac ctgatcgcgc ctccgcttga
     6901 ggcgatgtac gcgctcgacg cggcgctgaa ggccgcggac gtgaagctgg cggcgttcta
     6961 cggacctccc tcggagacca acttcggcgg cggcctgctc actggctcgc agtcggcgtg
     7021 caaagcggcc tgcgacgctt ttgcccaggc agtgatcttc gtcgcggaga acccggttct
     7081 ctactaacgg tttcatggag agcagagccc tgggaatgat cgaagtccgc ggaatgctcg
     7141 gcgcggtcga ggccgcggac gcggcgctga aatcggccgc cgtgcgtctg ctgcggtcga
     7201 ggaaagtcgg cggcgggctg gtgacggtct tcctgacggg agaggtcgcc gccgtccggt
     7261 cggcggtgga cgccgccgtc gcgtcattga acaggctcgg actgcagtgt tcgtcgcacg
     7321 tgatcccgcg tcttacgccg gatgttttca cgatgatcgc ggagggtggg ggcgcgcatt
     7381 cgaaacaggc cgcgcccgaa gcgccgcgcg ggccgtcttc taccggagat gccgtttctg
     7441 cggagaaaga tgtttccggg gagcgcccgt ccggctgcgt gatgaaggat gcggccggcg
     7501 aagaggacga tgtcgaggaa gccgaggacg agggcgatgt cggcgcggta tggagcgggg
     7561 acgcccccga cctcgagaac atgacggtga tggagcttcg tcgtcttgcc cgcaccgtcg
     7621 ggctggagac gatgacacga aagcagatac gcttcgccgg aaaggcggag ctgcagcgcg
     7681 aactgacgga gttttggaag aaccggggag gtgagagcga gtgacgcagc ttcaggacag
     7741 agatctgctc tccgtacagg aggtccgcaa cctcgtcgcg aaagcgaagg aggcgcagaa
     7801 gcgcctcgcc tccatgagtc agggcaagat cgacgaactc gtggcggcgg ttgccgaagc
     7861 ggcgtgccgg cacgcggagc ggctggggag gatggcgcac gaggagaccg gcttcggcaa
     7921 gcccgaggac aagaccctca agaacctctt cgccgcgaag agcgtgtacg aatcgatcaa
     7981 ggacctccgg acggtcggca tcgtccgcga ggatacgaag aacaggatgt tcgaggtcgc
     8041 cgtccccgtc ggcgtcatcg cggggctgat cccctcgacg aacccgacct cgacggcgat
     8101 ttacaaggcg atggtcgcgc tgaaggcggg gaacggcatc gtcttctctc cccacccgag
     8161 cgcga
//
LOCUS       DAOPKU010000057         8115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9407_length_8115_cov_5.983127, whole genome shotgun sequence.
ACCESSION   DAOPKU010000057 DAOPKU010000000
VERSION     DAOPKU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9407_length_8115_cov_5.983127"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PK849_02870"
     CDS             <1..763
                     /locus_tag="PK849_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HPE65097.1"
                     /translation="TAFLGSTTGGIAEMALLSMSMGADVASVTLLQVFRMLSAILVTP
                     LVCRKWTRWFGAGERSSCSSPSSLSVEPQFSDEEEAAALEPSVAPSAKGYALLAVVSL
                     IGGILGYIFRLPVGILTGAMFAVAATSLLRGEFPPMPNILRTVAQIGIGIIIGSNITI
                     STLHQFGELWLPVVALTAVLLVCSVLLAVLLHRMTGWDYPTCLLSSSLGGLSQMSIIS
                     EEMGADPLKVSILQTIRLLSILIVLPFLFSFLFGA"
     gene            complement(760..2205)
                     /locus_tag="PK849_02875"
     CDS             complement(760..2205)
                     /locus_tag="PK849_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPE65098.1"
                     /translation="MPLFLGVDLGTSSLKAAVLDEKGNIVARAKVPSVMNSPEKDFYE
                     VDAEATWWDGFLSLCTEAGKIVSLREIAAVCVSSVCASFVPVDEGGVPMHDAILYGID
                     KRASRQIERLNRRYGASLADRLGGMFTTHSILPKAIWLKEERPDAFRRTKYFMEPNNF
                     VTFRLTGERAWDYPSAAAAGLVDREKLTWAEDIFNEQGVSARSFAELRWPLSVLGSIS
                     EDASARTGLPAGIPVITGACDINAEAAAAGALSVGECVVVFGSTVSILLNTERPVRLP
                     GFVSGISVVEGMYRLGAATSSGSRFLQWVHSVIRSGDGKSVSSPTGILMVPWLDGART
                     PFHDPMAKMLFQGMDSGTSGGALLRAGKEALGYELDSLFAMLEQAAPVPETISVSGGL
                     SNDVELMEIVSAITGKKLRLHPSVDAACGDALLAAAGSGYVSLADCGRCARGARTISP
                     DMELHARFRPFVERFRKMTKQRGVPVGSSRG"
     gene            complement(2287..3216)
                     /locus_tag="PK849_02880"
     CDS             complement(2287..3216)
                     /locus_tag="PK849_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPE65099.1"
                     /translation="MKKYGVVVAVACLAALLVTGVAAAKQITVGFIATNFSAEAQARV
                     ANAFEKMAKEKNWDVKMLNSTGSIETQSNQLENLYQMKVDAVVMAMAHPLEIRPALDK
                     VLSANIPVITIDSGYVEGVVADITADNFAMGAKASTFLMDSIGGQGNIVVIKFEKHYG
                     TRRRGKVLDVVLSEYPGVKVLAEYSVVATKRFMDDTRAAMETYVTRFGDQIDGVWCAF
                     DQLAYVAGDVLQERGIKAVVVGTDGNQETMRRIGAGQMSATVAQPFEAMAEKAVELVQ
                     AIAAEGKSIEEATGGRKIFYMDAPLVDKTSLPE"
     gene            complement(3267..4226)
                     /locus_tag="PK849_02885"
     CDS             complement(3267..4226)
                     /locus_tag="PK849_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE65100.1"
                     /translation="MSQGKFQMQKMLTRYGTILALIVLCAGFSFAVPGFLAGENGMNI
                     LRQIALLAVISEGFTMCLIVGELDLAFPHIASLSGALVAGLIFGGMAPLPAVLLSLAT
                     GLLFGFFSGLLVTKIGIPSLITTLATGIIAGGMVYMYTRGVSFYGQMPPSFLALGRGM
                     VGPVPSLIFIMFVVVAVAQVFISKTRTGKYMQATGANQAASRLAGINTDRCKILALSL
                     SGLAAAFTGILLTSRLGSANPEGASGFMMDAFASALLGMTVLSVGRANPFGTFIGALM
                     IGVINNGMTLAGAPYYTQDIVKGLIILLSVTMTSLQAKRMAGN"
     gene            complement(4213..5709)
                     /locus_tag="PK849_02890"
     CDS             complement(4213..5709)
                     /locus_tag="PK849_02890"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPE65101.1"
                     /translation="MSILETRNLTKEFPGVRALSDFSFTLEKGEIHGLVGENGAGKST
                     LVKILAGVYTPTSGSFLLGGREMRFRSPRDAAERVGVVHQERELIPHFSGWENLFLGM
                     EFSSLGFLRKRRMREEALRFVEKYSMNIDLDLPASELGSGRQEMLAILKVLFRDPEVV
                     IFDEPTAPLSVKECESLFALIRDLKAKGLPILYISHHLSEVLHLADRITVLRNGRKVS
                     TVHAKDADEQTLIRLMIERDMESQYPKRSVSAGEEVFTMKRASSRKGRVRDVSIHVRR
                     GEIVGFAGLVGAGRTELAKAVFCGSRFEEGRMTLAGKPFSSRGPGESIRKGIAMIPEN
                     RREEGVVAEMTVGENLVLPLLKQLASFGFLRSRQCDLHANSVVDKLAIKISGLSQETR
                     TLSGGNQQKVSVGKWFGTPSELWIFDEPTQGIDVDAKREVYTIMGDLAERGAGVWFIS
                     SDLRELLAIADRIYVMKDFRIAGEFLPPFDDEEILGSMMGEGLRNVAG"
     gene            complement(5706..6515)
                     /locus_tag="PK849_02895"
     CDS             complement(5706..6515)
                     /locus_tag="PK849_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HPE65102.1"
                     /translation="MTVFETMFKVKKPILGMVHFPPLPGSPLYDGSAGMRKIRDTAMR
                     DAEALVKAGFDGIVFSNEGDRPYLSSVDHVTVAAMSRVISETAATFNVPFGLSVLADP
                     EAAVAIGAAVEADFVRIFLSWVFVGDWGIVDPDAGKLQRLRASLAPSMKVFANISGHT
                     EPLGGRKLEDIARGAVKFGLADAVCLAGTTAGSEIPEEDLRGARRGAEGYPVIAGTGV
                     RVENVERMLSLADGVIMGTSVKVDRDTFKPVDPLKAAEFIRCAKDARDKMQ"
     gene            complement(6502..7470)
                     /locus_tag="PK849_02900"
     CDS             complement(6502..7470)
                     /locus_tag="PK849_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HPE65103.1"
                     /translation="MPMYSDALLVKAAELYYYSRRSQAEIGRTLGVSTPTVSRILQEA
                     MERGVIEVRVRDILVRSSETEATLKRTFGLVDAVVVDMPQDADLNFLRKLLGKKASEL
                     LPRFCERGSTVGIGCGVTVREMIESLDRSRRFPDLRIVPLMGGWGKEEPERETNRLAS
                     SMGSILGCAFFYLLAPAIVSSPAVRDVFLAEPQIQLTTKLWNTVDTAIFSIGPELDAP
                     AYSYIPREYLDLEKAAKAGGVGDVLGRIIDEKGKELALPFNEHLMSIPFATLLKIPRR
                     VGIGGGAQKCRGVLAALRGGLVNILVTDYETGSYILQMEAMQRDSV"
     gene            complement(7767..>8115)
                     /locus_tag="PK849_02905"
     CDS             complement(7767..>8115)
                     /locus_tag="PK849_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65104.1"
                     /translation="DDSTSFRLLEAMLKKAGYSVLRAVDGEEALQAVRENPDVALVLM
                     DVKMPGMNGLEATRRIRRFNTVLPIIAQTAYAFPEDRTKALEAGCDDYISKPVERTAL
                     LKMIKRYCERKDA"
BASE COUNT         1610 a         2628 c         2244 g         1633 t
ORIGIN      
        1 aacggccttt cttggatcga cgacgggagg aatcgcggag atggcgcttc tctccatgtc
       61 catgggggcc gatgtcgcaa gcgtgaccct gcttcaggtc ttccgcatgc tctccgcgat
      121 cctggtcact cccctggtct gccggaaatg gactcgatgg ttcggcgccg gggagaggag
      181 cagttgttcc tccccttctt ctctttccgt tgaaccgcag ttttcggacg aagaggaagc
      241 tgccgcgctc gaaccttctg ttgcgccatc cgcaaagggg tatgcgttgc tggcggtcgt
      301 gtcgctcatc ggcggcatcc tcgggtacat tttcaggctg cccgtgggca ttttgaccgg
      361 agccatgttc gccgtagcgg cgacgagtct tctccgcgga gagtttccgc cgatgccgaa
      421 catactccga acggtggcgc agataggcat cggcatcatc atcggctcca acatcacgat
      481 ctccacgctt catcagttcg gcgagctctg gctgccggtt gtcgcgctca cagcggtctt
      541 gctcgtctgc agcgttctgc ttgccgtact gctccacagg atgacggggt gggactaccc
      601 tacctgcctt ctctcctcct cgctcggggg gctctcccag atgtccatca tctccgagga
      661 gatgggggcc gatccgctga aggtgagcat actccaaacc atccgcctgc tcagcattct
      721 catcgtgctg ccgttccttt tttcctttct tttcggcgct tagccgcggg agcttcctac
      781 cggcacgccg cgctgtttcg tcatcttgcg gaatcgttcg acaaaaggcc ggaagcgcgc
      841 gtgcagctcc atatcgggag agatcgtccg cgcgccgcga gcacacctac cgcaatccgc
      901 aagcgaaaca taccccgagc ccgccgccgc aagcagcgcg tcgccgcagg ccgcgtcgac
      961 cgaagggtgc aggcgcagtt tttttcctgt gatcgcggat acaatctcca tcaactcgac
     1021 gtcgttgctc agaccgcccg agacgctgat cgtttccggc acgggagccg cctgttccag
     1081 catggcgaag agcgagtcga gttcgtaccc caacgcctcc ttgccggcgc gcagaagcgc
     1141 acctcccgaa gtgccgctgt ccattccctg aaacagcatc ttcgccatcg gatcgtgaaa
     1201 cggcgtccgc gctccgtcca gccaggggac catcagaatg ccggtgggag aagatacgga
     1261 ttttccatcc ccgctccgaa taacggagtg cacccattgc aaaaagcgcg accccgaaga
     1321 ggtggccgcg ccgagccggt acatcccttc cacaacggat attcccgaaa cgaaccccgg
     1381 caaccggaca gggcgctccg tgttcagaag aatgctgacc gtcgagccga agacgacgac
     1441 gcactctccg acgctcaagg cgcccgccgc ggcggcctcc gcgttgatgt cgcacgcgcc
     1501 tgtgattacc ggtatccctg cggggagacc ggttcgcgcc gacgcatctt ccgaaatgct
     1561 tccgaggacg gagagcggcc accggagctc cgcgaaggaa cgtgccgaaa cgccctgttc
     1621 gttgaaaata tcctccgccc acgtcagttt ctctctgtcc acaagtccgg cagcggcggc
     1681 ggaagggtaa tcccacgcgc gttcgccggt cagccggaag gtgacaaagt tgttcggttc
     1741 catgaagtac ttcgtccgcc tgaacgcgtc ggggcgctcc tccttcagcc aaatcgcctt
     1801 gggaaggatg gagtgcgtgg tgaacatgcc tccgagacga tcggccagcg aggcgccgta
     1861 gcgccggttg agccgttcta tctggcgcga ggcccgtttg tcgataccgt agagaatggc
     1921 gtcgtgcatc ggaacgcctc cttcgtcgac gggaacgaac gatgcgcaga cggagctgac
     1981 gcacaccgcg gcgatctcgc gcaaagaaac gatttttccc gcctccgtac aaagcgaaag
     2041 aaaaccgtcc caccaggtcg cctcggcgtc cacctcgtaa aaatcttttt cgggggagtt
     2101 cattacgctc gggacctttg cccgcgcaac gatattcccc ttttcgtcca gaacggccgc
     2161 cttcagcgac gacgttccga ggtccactcc aagaaaaaga ggcactgcat acacctcctg
     2221 aaaaacggcg cggagagcat gtgatcgcca tccgcgccga aatgctgcgt acgtggaaga
     2281 aaggaactat tccggaagcg acgtcttgtc gacgagcggc gcatccatgt agaatatttt
     2341 ccgcccgccg gtagcttctt caatcgattt tccttcggcc gctatggcct gcacaagctc
     2401 aacagctttc tccgccattg cctcgaaagg ctgcgctacg gttgcggaca tctgtccggc
     2461 gcctatcctc cgcatggtct cctggtttcc gtccgttccg accaccacgg ccttgattcc
     2521 ccgctcctgc agcacgtccc ccgcgacgta cgcaagctgg tcgaaggcgc accatacgcc
     2581 gtcgatctga tcgccgaagc gggtaacgta cgtctccatc gccgctctgg tatcgtccat
     2641 gaagcgtttc gtcgcgacga cgctgtactc ggcgagcacc ttcacgcccg gatactccga
     2701 gagaacgacg tcgagcacct tccccctgcg cctggttccg tagtgtttct cgaacttgat
     2761 gacgacgata ttcccctggc cgccgatgct gtccatgagg aaagtggaag ctttcgcccc
     2821 catggcgaag ttatcggcgg taatatcggc gacgacgccc tccacgtacc cggagtcgat
     2881 cgtgatcacc ggaatgttcg cggagagcac cttgtccagc gcgggacgga tctccagcgg
     2941 atgcgccatt gccatcacca cggcgtccac cttcatctgg tagaggttct ccagctgatt
     3001 ggactgcgtt tcgatggacc ccgtcgagtt gagcatcttg acgtcccagt tcttctcctt
     3061 ggccatcttt tcgaacgcgt tggccacccg cgcctgcgct tcggcggaga aattcgtggc
     3121 gatgaacccc acggttatct gcttcgccgc cgcgactccc gtgacgagca acgctgccag
     3181 acacgctaca gcaacaacaa ccccgtactt tttcattccc tttccctcct tgaattgtgg
     3241 tttcctgtct ataaaagaag gcgaggtcag ttccccgcca ttctttttgc ctgaagagag
     3301 gtcatcgtca ccgaaagcaa tatgatcagt cccttcacga tatcctgagt gtagtacggc
     3361 gcgcccgcga gcgtcatgcc gttattgatc acgccgatca tcagcgcgcc gatgaacgtc
     3421 ccgaagggat tcgcccgtcc cacgctgagc accgtcatcc cgagcagcgc ggaggcgaac
     3481 gcgtccatca taaacccgga ggctccctcg gggttggccg acccgagacg ggaggtgagc
     3541 aagatgccgg tgaaggctgc ggcaagaccg gaaagtgaga gcgccaaaat cttgcaccgg
     3601 tccgtgttga taccggcaag ccgggaagcg gcctgattcg cccccgtggc ctgcatgtac
     3661 ttccccgtgc gggtcttcga gatgaacacc tgcgcgaccg ccaccacgac gaacatgatg
     3721 aaaatgagag aggggaccgg cccgaccatc ccccgcccga gcgcgaggaa cgaagggggc
     3781 atctgcccgt agaaagagac gccgcgcgtg tacatataca ccatcccccc ggcgataatg
     3841 cctgtggcca gcgtggtgat gagcgacgga atgccgatct tcgtcacaag gagccccgag
     3901 aagaaaccaa agagaagtcc ggtcgccaga gagagcagca ccgcgggaag cggcgccatt
     3961 cccccgaaga tcagccccgc caccagcgcg ccggaaaggc tggcgatgtg cggaaaggcc
     4021 aggtccagct ctcccacgat gaggcacatc gtgaatcctt ccgagatcac cgccaaaagg
     4081 gcgatctgcc tgagaatatt cattccgttc tcacccgcca aaaaccccgg caccgcaaaa
     4141 gaaaaccctg cgcacaagac gatcagggcg agaatcgtcc cgtaccgcgt cagcattttt
     4201 tgcatttgaa atttaccctg cgacatttcg gagcccctcc cccatcatgc ttccaagaat
     4261 ttcttcatcg tcgaacggag ggaggaactc ccccgcaata cgaaaatcct tcatgacgta
     4321 tatccggtcg gcgatggcaa gcagctcgcg aagatccgag ctgatgaacc acacgcccgc
     4381 ccctctttcc gcgagatcgc ccatgatggt gtagacctcg cgttttgcgt ccacgtcgat
     4441 cccctgcgtc ggctcgtcga aaatccagag ctccgaaggg gtcccgaacc attttccaac
     4501 ggagaccttc tgctggtttc ctccgctgag cgtcctcgtc tcttgcgaga gaccggagat
     4561 cttgatggca agcttgtcca cgacgctgtt cgcgtgcaga tcgcactgcc tcgatcgaag
     4621 aaagccaaac gacgcgagct gcttcagaag agggagcacg aggttctccc ccaccgtcat
     4681 ttcggcgacg acgccctctt cgcgcctgtt ctccgggatc atcgcaatcc cctttcgtat
     4741 gctctcgccc ggaccgcggg aggaaaaggg tttccccgcc aacgtcatcc gcccctcctc
     4801 gaagcgagag ccgcaaaaga ccgctttcgc caattccgtg cggcctgcgc cgacaagccc
     4861 ggcgaacccg acgatctcgc ctctgcgaac atgtatcgaa acgtcgcgca ctcttccctt
     4921 ccgggaggag gctcgtttca tcgtgaacac ttcctccccc gcggaaacgc tccgtttggg
     4981 gtactggctc tccatatccc gctcgatcat caaacggatc agcgtctgtt cgtccgcatc
     5041 cttcgcgtgc accgtggaca cctttctccc gttccggaga accgtgatcc ggtcggcgag
     5101 gtgcaacacc tccgaaaggt ggtgggagat atacaagatg gggagccctt tcgccttcag
     5161 gtccctgatc agagcgaaga gactctcgca ctccttcacg ctcaagggcg ccgtgggttc
     5221 gtcgaaaatg acgacctccg gatcacggaa gagcaccttg aggatggcga gcatctcctg
     5281 gcggccgctg ccaagctccg aagcgggaag atcgaggtcg atgttcatcg aatacttctc
     5341 gacgaaacga agcgcctctt cgcgcattcg tcttttccgc agaaaaccga gcgaggagaa
     5401 ctccatcccg agaaagagat tctcccatcc tgaaaagtgc gggatcagtt cccgctcctg
     5461 atggacaacg cccacccttt cggcagcgtc gcgcggcgac cggaaacgca tctctctccc
     5521 gccgagcagg aacgacccgg aagtcggcgt atacacgccg gcgagtatct tcaccagggt
     5581 ggacttcccg gcgccgttct ctcccacaag accatgaatc tcccccttct ccagcgtgaa
     5641 ggagaaatcg gaaagcgccc ggactccggg gaattccttg gtgaggttcc gggtctcgag
     5701 aatactcatt gcatcttatc cctggcgtcc ttcgcgcagc gtatgaactc cgccgctttc
     5761 agcgggtcga ccggcttgaa ggtgtccctg tccaccttga cgctcgtccc catgatgacg
     5821 ccgtcggcaa gggagagcat tctttcgacg ttctccacgc gtactcccgt cccggcgatc
     5881 acgggatacc cttccgctcc gcgcctggcg ccgcgcagat cctcctcggg aatttcgctc
     5941 cccgccgtgg ttcccgcgag gcagaccgcg tcggcgagtc cgaacttgac cgctcctctc
     6001 gcgatatcct ccagtttccg ccctcccaga ggctccgtat gccccgagat gttggcgaac
     6061 actttcatcg agggagcaag cgacgcccgc aggcgctgga gctttcccgc gtcggggtcc
     6121 acgatccccc agtcgcccac gaaaacccat gagaggaaga tgcggacgaa atcagcctcc
     6181 acggccgcgc cgatcgccac ggccgcctcc ggatcggcca gtacggagag gccgaagggg
     6241 acattgaacg tggcggcagt ctcggaaatc actctgctca tcgccgccac cgtcacgtgg
     6301 tccacggagg agagatacgg cctgtcgcct tcgttgctga aaacgattcc gtcgaagccc
     6361 gccttgacga gcgcttcggc gtcgcgcatc gcggtatcgc gaattttcct cattcccgcg
     6421 cttccgtcat acagaggaga gccgggaagc ggcgggaaat gcaccatccc caaaataggc
     6481 ttcttcacct tgaacatcgt ttcaaacact gtcacgttgc atcgcctcca tttgcagtat
     6541 gtacgatccc gtctcgtagt cggtgaccag tatattgaca agccccccgc ggagcgccgc
     6601 gaggacgccg cggcattttt gcgcccctcc tccgataccg acgcgccggg ggattttcaa
     6661 caacgtcgcg aagggaatac tcatcagatg ttcattgaag ggcagggcaa gttcctttcc
     6721 tttctcgtcg ataattctgc cgagcacgtc gcccacccct ccggctttcg cggctttttc
     6781 gaggtcaaga tactccctcg gtatgtagga gtacgccggg gcgtcaagct ccggcccgat
     6841 ggaaaagatc gccgtatcca cggtgttcca cagcttcgtg gtcaactgga tctgcggttc
     6901 cgctaaaaag acgtcccgga cggcgggcga gctgacgatg gccggagcga gaagatagaa
     6961 gaaagcgcat ccaagaatac tccccatgga actggcgagg cggttcgtct cccgctccgg
     7021 ctcttccttc ccccaccctc ccatgagcgg gacgatgcgg agatcgggga agcggcgcga
     7081 gcggtcgagc gactcgatca tctcgcgtac ggtgacgccg caccctatcc cgacggtact
     7141 tcctctctcg cagaagcggg gcagcagttc gctcgccttc tttccaagca gtttccgaag
     7201 aaagttcaag tccgcatcct ggggcatgtc gaccaccacc gcgtcgacaa gcccgaaggt
     7261 tctcttcagc gtcgcctcgg tctccgagga gcggacaagg atgtcgcgaa cgcggacttc
     7321 gatgacgccg cgctccatcg cctcctgaag aatccgagac actgtggggg tggaaacccc
     7381 gagagtcctt cctatctccg cctgtgatct ccgggagtag tagtagagtt cggctgcctt
     7441 taccagaagc gcgtccgaat acatcggcat cccccttgaa aaaaattcct gaaataattt
     7501 tctatctctc ttctgtataa gtataggaga gttccgcaaa ttgtcaatcg ctcggaagtg
     7561 aattgaaagt ggaggttgta taaacgacgc tcccctccgt ctgtgtacca cggaggggag
     7621 cgtatcggaa gtcgcgcaca acatgttttg gaggggccaa cgcgaaaggt tctctcgtcc
     7681 ataaaatccc cttcgttagt acccgggaaa gactgctttc acggaagagg ggcaatttcc
     7741 cttcatttca aagagagggg gggcgctcaa gcgtctttcc tttcgcagta gcgcttgatc
     7801 atcttcagca gcgccgtccg ttcgacaggt ttggagatat agtcgtcgca ccctgcttcc
     7861 agcgcttttg tgcggtcttc cggaaaggcg tacgccgtct gcgcgatgat cgggagcacg
     7921 gtattgaagc gccgtatccg tcttgttgcc tccagtccgt tcattcccgg catttttaca
     7981 tccatcagaa caagagctac gtccgggttt tcgcggacag cctgcagcgc ttcttccccg
     8041 tcgacggcgc gaagcacgga ataccccgcc ttcttcagca tcgcctccaa aaggcggaaa
     8101 ctggtcgagt cgtcc
//
LOCUS       DAOPKU010000058         8110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9412_length_8110_cov_5.177281, whole genome shotgun sequence.
ACCESSION   DAOPKU010000058 DAOPKU010000000
VERSION     DAOPKU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9412_length_8110_cov_5.177281"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK849_02910"
     CDS             <1..195
                     /locus_tag="PK849_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65105.1"
                     /translation="VDHCVHDLRAVFRQVEGEDLVSEGDDVLRERFRLFFPGELPVDE
                     KKQPVGGLPVEDGRNAAATP"
     gene            complement(185..622)
                     /locus_tag="PK849_02915"
     CDS             complement(185..622)
                     /locus_tag="PK849_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPE65106.1"
                     /translation="MPGMKEEVNVRLVNQKVQFIGVSSANPEQPLTFDYAPPMGDGAG
                     FNGLELLLMSLCGCSATAVVYLLRKMRKTVSGFEVQAEGIRKETHPIKFEKITLRFIL
                     KSPDAQEEDIQKAVQLTEETYCPVWQMIKNNVDVVPEYTIVRE"
     gene            1043..2134
                     /gene="rsgA"
                     /locus_tag="PK849_02920"
     CDS             1043..2134
                     /gene="rsgA"
                     /locus_tag="PK849_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPE65107.1"
                     /translation="MNTKILERIGFDRRFADEASMYPGFYAGRVVSQSKDLYRVTTEE
                     SDLTAEISGKFRFEVRTPSDYPAVGDFVLLDRNEDSSGNAIIHRVLTRKSVFIRRAAG
                     TSHEEQVVAANIDTVFICMSLNNDYNLRRVERYLGVAWASGAAPVVVLTKADLCDDVA
                     ERLAELDSVACGVDVLVTSSLTEDGYASVLEYLGAGRTIAFIGSSGVGKSTLINRLIG
                     RDALETRGLRNDDKGRHTTTRRELIALPNGGIVIDTPGMREMGLEGADLAKTFADIDE
                     LAAQCRFRDCTHANEPHCAVQRAIRDGLLSEERLAGYLKLKKEARYEGLNSRQIETEK
                     LSSMFAEIGGIKNARKFAKEKNRTKHGYC"
     gene            complement(2302..3591)
                     /locus_tag="PK849_02925"
     CDS             complement(2302..3591)
                     /locus_tag="PK849_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE65108.1"
                     /translation="MESLHNRSKGAGEWILENIVTLIFIAFTLFGFMVSSGVSVGYFF
                     SELSSRIFRNSFLVLSLIIPVLAGLGLNFGIVIGAMSGQVAISIARYFEMGGISGLLF
                     CFLVAALVAALCGYFTGKLYNKTRGQEMIASLIVGFFANGIYQFLFLFVVGVVIAVPA
                     THPMIKPDGIGIRMTVDLVPVKQGGLKYALENIYQIPFVHALLIIALGLLALLLIRYY
                     WNKRKGYGYRNRMSSLIVNGTLCLALAGVAVHAMVTKSQLMMVRKVPVVTGLLIIGLC
                     VFTVLIMKTKLGQDFRSVGQSQHTAEVSGINVDRTRIIATMISTVLAAWGQIIYLQNM
                     GTLNTYNAHTQIGMFSVAALLVGGASTSKASITHAIFGTILFNAMFIMSPEIGQSLFG
                     QAILGEYFRTFMVYGVIGVALGLHVWNTNKKSRITVE"
     gene            complement(3591..4664)
                     /locus_tag="PK849_02930"
     CDS             complement(3591..4664)
                     /locus_tag="PK849_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE65109.1"
                     /translation="MNGNGNGNNRLRCVGGVLGGIGLPTLIIGGFWVFTVIAGHFVGI
                     SMATLVSDTIKRAGMNGILVLAMVPAIQSGTGPNFALPIGIVCGLLGLVTAIELNLMG
                     MAWFLTSCAIAIPLAAIVGYGYGKLMNAVKGSEMTIATYTGFSIVALMCLAWLMIPFK
                     NPKMGWFIGTGLRETIQLDAVGGAQILNNFLMFTIGEVIIPTGLLLTFAFFSFLVWLF
                     FRSKRGIAISAGGINPKFARATGLDIDGNRVFANVISTVLGAIGIIVYSQSYGYAQLY
                     TAPLMMAFPAVAAVLIGGATASRAKVSHVIIGVVLFQGLLTTALPVANEVFAGTDLSE
                     IMRMIIQNGIILYALTQVKRGVQ"
     gene            complement(4666..>5032)
                     /locus_tag="PK849_02935"
     CDS             complement(4666..>5032)
                     /locus_tag="PK849_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPE65110.1"
                     /translation="LQHAGALSGGNQQKVCVARALTQDPIFLFVSEPTRGIDIGAKKL
                     ILDLLLKLNEEFGMTIVMTSSELAELRSICDRIVIVCEGKVEGVLPPDASDADFGLMM
                     SGSRVKKAEASPKERVSHV"
     assembly_gap    5033..5132
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5133..6437)
                     /locus_tag="PK849_02940"
     CDS             complement(<5133..6437)
                     /locus_tag="PK849_02940"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPE65111.1"
                     /translation="MSESLLQFKNVSKNYYGNSVLTGINFDVKEGEIHALIGENGAGK
                     STLMNILFGMPVITSTGGYEGDVLIDGKKISFKSPHEAMYAGIGMVHQEFMLIPGFSI
                     TENIKINREITTPNVVSRVLGKRLETLDMVTMNADARKALDKLDMGVDEFIMVAGLPV
                     GHMQFVEIAREIDKTGIKILVFDEPTAVLTESEAQNLLAAVKRLAAKGIGIIFISHRL
                     DEIVTVADRVTVLRDGELVATKEIKDTNAVEIAALMIGRKVAISRVQSETRKVSDEIM
                     MSIRDLHVGMPGEMVKGIDLEIKKGEIVGIGGLAGQGKLGIANGVMGTYPSRGEVLFN
                     GKRLNLDDPHGTIASGIGFVSEDRKGVGLLLNESIELNIAFTAMQVGNKFLKKSGPFR
                     FQDDAAIRRHALKMIKDLDIRCESPLQHAGALSGGNQQKVCVA"
     gene            complement(6584..7735)
                     /locus_tag="PK849_02945"
     CDS             complement(6584..7735)
                     /locus_tag="PK849_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HPE65112.1"
                     /translation="MKKVVGILLVLAVVAGFAYYYMAPKPGPVEPPVKPTAGKVAIVT
                     NTVSQNEEEYRSAQEMVAKYGDRVVHVTWPDNFMAEQEQMVSVVAKLAYDPDVKAVII
                     NQAVPGTNAAVDKLLETRKDVFIVYCTPQENPPDVAARAQIIMMPDELAMGNTIPTQA
                     KKLGAKVFVHYSFPRHMSQVLLSSRREIMKKKCAELGLEFVDATAPDPTGDSGLPGAQ
                     QFILEDVPKMVAKYGKDTAFFSTNCAMQIPLIKSVVDTGAIYPQPCCPSPYHGFPTAL
                     GIESDLSKQDNIKHVIDETRRILKEKGMTGRLSTWPVPVAMMGTVAGTEYAFKVIDGE
                     VPFDKLDVKVLEQCMSDYAKVAVETNPYVDEAGKKYDNFLFVLMDFLTY"
BASE COUNT         1773 a         2283 c         2196 g         1758 t
ORIGIN      
        1 gttgaccact gcgttcatga tctgcgagcc gtgttccggc aggtcgaagg cgaggacctg
       61 gtatccgaag gggacgacgt cctccgcgaa cgcttccgcc tcttctttcc gggagagctt
      121 cccgtggacg aaaagaaaca gcctgtcgga gggctccccg tagaggacgg ccggaatgcc
      181 gccgctactc cctgacgatt gtatattccg gtacgacgtc gacattgttc tttatcatct
      241 gccacacggg gcagtacgtt tcctcggtaa gctgcacggc cttttgaatg tcctcttctt
      301 gtgcgtccgg cgatttcagg atgaaacgga gcgtaatctt ctcgaatttt atcggatgcg
      361 tctccttccg gattccttcc gcctgaactt cgaatccgga gacggtcttc ctcatttttc
      421 gcaacagata gacaacggcg gtggcgctgc agccgcacag gctcataagg agcagctcga
      481 gcccgttgaa ccccgccccg tcgcccatcg gaggcgcgta atcgaacgtg agcggctgtt
      541 cgggattggc agatgaaacg ccgataaatt ggaccttctg gttcactaac cggacgttga
      601 cttcttcttt cattccaggc atttttcttg ctccttcgat cttttagaat gtgattcgtt
      661 cgagagcaca gcctctcgta cagatggcgc agagcgcggg aaggcgatat cctcgggtgc
      721 agcggcgaaa cagtacacgg aatagagcac tatcaaagcc actcttgaag aaaactttat
      781 ccctgcgccc ttttaccata tcctatcagt taaatcaagt tttttgttca attttttgtt
      841 tattgccgcc cggcgtcttg caatcacact ctttcgtgct atcatcagac ggcggaaatt
      901 attcggaaac agaaggagtg gtgacgtatg ttgttgaaga gggtcgcgcc ggaggttgtc
      961 aaagtccggg aggcgactgc acgctgagtt gatgtatccg cctcccgaaa gtcattgaaa
     1021 aatacttttt ggagggaaaa cagtgaacac taaaatttta gaacgtatcg gcttcgaccg
     1081 gcgcttcgcc gacgaagcaa gcatgtaccc cggattctac gccgggcgcg tcgtgtcgca
     1141 gtcgaaggac ctgtacaggg tgacgacgga agagagcgac ttgacggcgg agatctcggg
     1201 gaaatttcgt ttcgaggtgc gcacgccgtc ggactatccg gcggtgggcg attttgtgct
     1261 gctcgaccgg aacgaggatt cgagcggcaa cgcgatcatc catcgcgtgc tgacgaggaa
     1321 gagcgtcttc atccggaggg cggcggggac gtcgcacgag gagcaggtgg tggcggcgaa
     1381 tatcgacacc gtcttcatct gcatgtcgct gaacaacgac tataacctgc ggagggtgga
     1441 gcgctacctg ggggtcgcct gggcgagcgg cgcggcgccc gtggttgtgc tgacgaaagc
     1501 cgacctgtgc gacgacgtcg cagaaagact ggcggagctg gactctgtgg cctgcggggt
     1561 tgatgtactg gtcacgtcga gtctgaccga agacgggtac gcatctgtcc ttgaatacct
     1621 gggcgcgggg cggacgatcg ccttcatcgg ctcgtcgggg gtcggcaagt cgacccttat
     1681 aaacaggctg ataggcaggg acgctctgga aacgcggggc ttgcggaacg acgacaaggg
     1741 gcggcacacg acgaccaggc gcgaactgat cgcactgcca aacggcggca tcgtcatcga
     1801 cacgccgggg atgcgggaga tggggctcga aggcgccgac cttgcaaaga cgttcgccga
     1861 catcgacgag ctggctgcgc agtgccgctt ccgcgactgc acgcacgcaa acgagccgca
     1921 ctgcgcggtg caacgcgcca tacgggacgg cctgctctcc gaggagcgcc ttgccggcta
     1981 tctcaagctg aagaaggaag cccgctacga ggggctgaat tcgcggcaaa tcgagacgga
     2041 gaagctctct tccatgttcg ccgagatcgg cggcatcaag aacgcgcgca agttcgccaa
     2101 ggagaagaac agaacgaagc acggctattg ctagggaacc tctgaaaaat cagaaatgct
     2161 ccctcgggaa gccgctttca ccgaatttcc cgagggaacg cgcttctttc cgagaatctc
     2221 tcaatgcaac gcccccgtcc ttccggttca ggaaagacgg gggcgttgcg ttgtttcgac
     2281 ggagcagcac cctccgccgg gttattccac ggttattctg ctctttttgt tcgtattcca
     2341 gacgtggagt ccgagggcca cgccgatgac gccgtacacc atgaaggttc tgaagtattc
     2401 tccgaggatc gcctgaccga agagcgactg ccctatctcg ggcgacatga tgaacatggc
     2461 gttgaacaga atggtgccga agatggcatg ggtgatgctc gccttcgagg tggacgcgcc
     2521 gcccacgaga agggcagcga ccgagaacat gcctatctgg gtatgcgcgt tgtaggtgtt
     2581 cagcgtcccc atattctgga ggtagatgat ctgcccccag gccgcaagaa cggtcgagat
     2641 catagtagcg atgatcctcg tccggtcgac gttgattccg gagacctccg ccgtgtgctg
     2701 gctctgcccg acggaacgga aatcctgccc cagcttcgtc ttcatgatga gcaccgtgaa
     2761 gacgcaaagg ccgatgatga gcagccccgt cacaacgggt accttgcgca ccatcatgag
     2821 ttgcgacttc gtgaccattg cgtggacggc gacgcccgcg agagcaaggc acaacgtccc
     2881 gttgacgatg agagacgaca ttctgttcct gtacccgtac ccttttctct tgttccagta
     2941 gtagcgaatg agcagcagcg caagaagacc gagggcaatg atcaggagcg cgtggacgaa
     3001 cgggatctgg tagatgtttt caagcgcgta cttcagcccg ccctgcttca ccggcacgag
     3061 gtcgacggtc atgcggatgc cgatgccgtc cggtttgatc atcgggtgcg tcgcgggaac
     3121 agcgatgacg acgccgacga cgaagaggaa gagaaactga tagatgccgt tcgcaaagaa
     3181 accgacaatc aggctggcga tcatctcctg accccgcgtc ttgttgtaca gcttcccagt
     3241 gaagtacccg cagagcgcgg caacaagcgc cgccaccagg aaacagaaca gaagccccga
     3301 aatgccgccc atctcgaagt agcgcgctat ggatatggca acctgccccg acatcgcccc
     3361 gatgacgatg ccgaaattca gcccgaggcc cgcaagtaca ggaatgataa gcgaaaggac
     3421 gagaaaggaa tttctgaaga tcctgctgga aagctccgag aagaagtagc cgacggagac
     3481 gccgctcgac accatgaagc cgaaaagcgt gaatgcgata aaaatcagcg tgacgatgtt
     3541 ctcaagaatc cactcgcctg cacccttgga tctgttgtgt agactttcca ttattgcaca
     3601 cctctcttca cctgggtcag ggcgtagagg atgattccgt tctgaataat cattctcatt
     3661 atctccgaca ggtcggtacc ggcaaatact tcgttcgcca cgggaagcgc cgtggtcagc
     3721 aagccctgga agaggacaac gccgataata acatgggata ctttggcgcg cgacgcggtg
     3781 gcccctccga tgaggactgc ggcaaccgcc gggaacgcca tcatcaacgg cgccgtgtac
     3841 agctgcgcgt atccgtagct ctgggagtag acgatgatgc cgatcgcacc cagaactgtg
     3901 gagatcacgt ttgcgaagac cctgttgccg tcgatatcaa ggccggtagc ccgcgcgaac
     3961 ttcgggttga ttcctcccgc ggaaatcgcg attccccgtt tcgagcggaa gaagagccat
     4021 acgagaaatg aaaaaaacgc gaacgtcaga agaagccccg tcgggatgat cacttccccg
     4081 atggtaaaca tcaggaaatt attgagaatc tgcgcgcccc cgaccgcgtc gagctgaatt
     4141 gtctctctga gtccggtgcc gatgaaccag cccattttgg ggtttttaaa cgggatcatc
     4201 agccacgcga gacacatcag cgcgacaatc gaaaaccccg tataggtggc aatcgtcatc
     4261 tcggatccct tcaccgcgtt catcagcttg ccatatccgt agccgacaat cgcggcgagc
     4321 ggaatcgcga tggcgcacga cgtcagaaac catgccattc ccatcaagtt cagctcgata
     4381 gcggtgacaa gaccgagcag cccgcagacg atgcctatcg gcagcgcgaa gttcggcccc
     4441 gttcccgact ggatcgcggg taccatggca agaacgagga ttccgttcat ccccgctcgt
     4501 tttatcgtat cgcttacaag agtcgccatc gatatgccga caaaatgccc ggcgattacc
     4561 gtaaagaccc agaatccccc gataatcagc gttggaagac caataccgcc cagcactccg
     4621 ccgacgcagc ggagacgatt attcccgttg ccgtttccgt tcatctcaga catgcgatac
     4681 tctctccttc ggtgaagctt ccgctttttt gactctgctg ccggacatca tcaggccgaa
     4741 atccgcgtcc gaggcatccg ggggcagtac gccctcgacc ttgccctcgc agacgatgac
     4801 gatcctgtcg cagatgctcc gaagctccgc gagctccgac gaggtcatga caatcgtcat
     4861 tccgaactcc tcgttcagtt tcaaaagcag gtcgagaatc agcttcttcg cacctatgtc
     4921 gatacctcgt gtaggctcgg agacgaaaag aaagatcgga tcctgcgtca gcgccctcgc
     4981 gacgcagacc ttctgctggt tgccgccgct caaggcgccc gcgtgctgca gannnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcgacgc agaccttctg ctggttgccg
     5161 ccgctcaagg cgcccgcgtg ctgcagaggc gattcgcaac gaatatcgag atcctttatc
     5221 attttcagcg catggcgtcg aatcgccgcg tcgtcctgaa aacggaacgg accgcttttt
     5281 ttcaagaatt tgttcccgac ctgcatcgcg gtgaaagcga tgttcagctc gatggattcg
     5341 ttgagcagca gcccgacgcc ctttctgtcc tcgctgacga aaccgatccc cgaggcgata
     5401 gtaccgtgcg gatcgtcaag attcagccgc ttgccgttga acagcacttc gcccctcgac
     5461 gggtaggttc ccatgacgcc gttcgcgatc cccagttttc cctggccggc gagtccgcct
     5521 atccccacga tctcgccctt tttgatctcg aggtcgatcc ccttgaccat ctcgccgggc
     5581 attccgacat gcagatcacg tatgctcatc atgatttcgt ccgatacttt gcgcgtctcg
     5641 gactgcactc tgctgatcgc caccttgcgg ccgatcatca gggcggctat ttccaccgcg
     5701 ttcgtatcct tgatctcttt cgtggcgaca agttctccgt cgcgcaaaac cgtgactctg
     5761 tccgcgacag taacgatctc gtcgagcctg tggctgatga aaataatgcc gattcccttc
     5821 gcggcaagcc gtttcacggc ggcaagcaga ttctgcgcct cactctccgt aagcaccgcc
     5881 gtgggctcgt cgaagacgag aatcttgatg cctgtcttat ctatctcgcg ggctatctcg
     5941 acgaactgca tgtgccccac gggaagcccc gcgaccatga tgaattcgtc gacgcccatg
     6001 tcgagcttat ccagcgcttt gcgggcatcg gcgttcatag tgaccatgtc gagcgtttca
     6061 aggcgtttgc cgagaactct ggagacgacg ttcggagtgg ttatttccct gttgattttg
     6121 atgttctccg tgattgaaaa ccccggaatc agcatgaact cctggtgaac catgccgatt
     6181 cccgcgtaca tagcctcatg cggcgacttg aacgaaatct tctttccgtc gatcagcaca
     6241 tcgccctcgt agccgcctgt gcttgtgatc acgggcatcc cgaagaggat attcatcagc
     6301 gtggacttcc cggcgccgtt ctcccctata agcgcgtgta tctcgccctc tttgacatcg
     6361 aagttgatgc cggtcaatac gctgtttccg tagtagttct tggaaacatt tttgaactgg
     6421 agaaggcttt cgctcaacgc attacccccc gtatctcaaa aaatataaaa gctgcaataa
     6481 aagagggacg aacggattcg acaccagcaa ttcgcttcgg tgaaggatcc attcgtcccc
     6541 ggacgctaca tttgttacag cttcttttta tgcaaccctt cgattagtag gtcaggaagt
     6601 ccataaggac gaacaggaag ttatcgtatt tctttcccgc ctcgtcgaca tacggattcg
     6661 tttccaccgc gaccttcgca taatcggaca tgcactgctc cagaactttg acatcgagct
     6721 tatcgaacgg gacttcgccg tcgatgactt tgaaagcgta ctcggtaccg gcgaccgtac
     6781 ccatcattgc aaccggcacg ggccacgtgg aaagtcttcc agtcatcccc ttctccttga
     6841 ggattctgcg cgtctcgtcg ataacgtgct tgatgttgtc ctgcttggac aaatcggact
     6901 cgataccgag agccgtcggg aatccgtggt acggagacgg gcagcagggc tgcggataga
     6961 ttgcgcccgt gtcgacgacg gacttgatca gaggaatctg cattgcgcag ttcgtggaga
     7021 agaacgccgt gtccttgccg tacttcgcga ccatcttcgg cacgtcctcg aggatgaact
     7081 gctgcgcgcc gggaaggccg gagtcgcctg tcgggtccgg agccgtcgcg tcgacgaatt
     7141 caagtccgag ttcggcgcac ttctttttca tgatttctct gcgcgaggaa agaagaacct
     7201 gggacatgtg acgcgggaac gaatagtgga caaaaacctt tgcgccaagc tttttggcct
     7261 gggtgggaat cgtgttgccc attgcgagtt cgtcgggcat catgataatc tgcgccctcg
     7321 cggccacatc ggggggattc tcctgcgggg tgcagtacac gatgaagaca tcttttcttg
     7381 tttcaagaag tttgtcaacc gccgcgttcg ttcccggaac agcctggttg atgataaccg
     7441 ccttcacatc cggatcgtac gcaagcttgg ccacgacgct gaccatctgc tcctgctccg
     7501 ccatgaagtt gtcgggccac gtaacgtgaa caactctgtc tccgtacttc gcgaccattt
     7561 cctgtgcgga acgatactct tcctcgttct gagaaacagt gttcgtcacg atcgcgacct
     7621 tacctgccgt aggcttaacg ggaggctcga ccgggccggg tttcggtgcc atataataat
     7681 aggcgaaacc ggcaaccacg gcgagaacta ataaaatacc caccactttc ttcattcagt
     7741 accctcctat ccaattgtaa gttcaaaacc tttttctaag aaaagatacc gcccagcgcg
     7801 ccccttgtca agcgctaact tttctttgca ataatgacct tataacaatt tcatcaaatc
     7861 aacacagtaa atatttgaac caaggtgcct aaaagagaca ataactcacc caagtttcgc
     7921 atgtcttgct ctttttcgga aagttatttt ggacaatggc cttttcaggc ggtagaatag
     7981 agcgcagcgg ttgtttcgca actctttccg gagggatgtt catgatcaat caaaaaaggt
     8041 taaaaaagct tgccgatgag ttacacaagc tgggtttgga cgcaatctac cttggtcctt
     8101 caacggacct
//
LOCUS       DAOPKU010000059         8086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9446_length_8086_cov_3.922177, whole genome shotgun sequence.
ACCESSION   DAOPKU010000059 DAOPKU010000000
VERSION     DAOPKU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9446_length_8086_cov_3.922177"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PK849_02950"
     CDS             complement(<1..219)
                     /locus_tag="PK849_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE65113.1"
                     /translation="MPVRVFLADDHPLTRAGISAYLAQETSVELVGEAEDGETAWKLI
                     SEIRPDVALLDIRMPGEDGVSVARRVKES"
     gene            complement(325..909)
                     /locus_tag="PK849_02955"
     CDS             complement(325..909)
                     /locus_tag="PK849_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPE65114.1"
                     /translation="MNFTRENALDLLKKHNKDEGHIKHALAVESSMKFFAEKMGGDVE
                     KWGMAGLLHDIDWEEMQETPERHTIEGARWLSEEGYPEDIVRAVLAHGWGICSDVKPE
                     NDMEKVLFTIDELTGLVMTAALVRPSRSVQDLEVKSVKKKWKDKAFAKGVDRELIVKG
                     AEMIPMPLDTVIEWVILAMRRIEPDLGLGSAAGA"
     gene            complement(906..1436)
                     /locus_tag="PK849_02960"
     CDS             complement(906..1436)
                     /locus_tag="PK849_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HPE65115.1"
                     /translation="MSFSRPEWDVYFMMMASVAGTRSTCLRRRVGAVVVRDRQIVSTG
                     YNGAPKGLPHCADAGCLRTLLSIPSGERHEMCRGSHAEMNAIAQAAAAGVCTAGGQIY
                     CTHEPCSFCTKAIINAGIERVIFLHPYPDTLAREMRSQASIEVTCLSPDFFATAGALL
                     GDLFSPAENARRNETE"
     gene            1585..2820
                     /locus_tag="PK849_02965"
     CDS             1585..2820
                     /locus_tag="PK849_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE65116.1"
                     /translation="MFSSFPKLEKKVWSWCTYDVGNSAFATTIMAAIYPVYFREVASR
                     GLAPNISTAYWAYTASAALFLSAFSSPLLGALADIQGSKKRLLAIFTGIGVLSTGCMA
                     FIGPGDWLLALILMAAGTIGFTISIIFYDALLPSLVPPERVDRVSAQGYAFGYLGGGV
                     LLAINFLFIALLPGTIGARLSFLSVAFWWGGFSLPVLFNVAEPPVDGERVDTNTAFRG
                     SVKRLQKTFREIRSFRNLFLFLVAFWFYNDGIGTIIRMAAIYGSQLGISMNHLVGALL
                     LTQFVGIPFSLAFGTLASHMGSKKTILIGLYFYLCLIVAAVFISKPWHFWALAFSVGM
                     VQGGTQAISRSFFASMIPPGRTAEFFGFYDISSKFSGILGPAIFGFITQVSGSSPLAI
                     AILSYTFILGIVILRKIEG"
     gene            2829..3557
                     /locus_tag="PK849_02970"
     CDS             2829..3557
                     /locus_tag="PK849_02970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPE65117.1"
                     /translation="MHTSFLRTLCLILILLVSLNQNRAEASPPVYSGSRMVHTIAITF
                     DDGPHQILTPALLDTLDALDVKASFFLVGKMVDRYPALVREIYARGHTVANHSYWHNN
                     SSLLSPEEFLDDLRRCSESIERNIPVRVRFFRPPGGNYTKNVLSLVEEAGMRTVLWDI
                     NSRDYTGVSAARMTARILSKVSPGSILLFHSGVSSTINALPEIVSSLRSKGFDFVTLD
                     EMLSGYSALLPHTTGSESSTLASK"
     gene            3587..4612
                     /locus_tag="PK849_02975"
     CDS             3587..4612
                     /locus_tag="PK849_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPE65118.1"
                     /translation="MASFLVISTGGTIASRAGSNGLVPSLPGEELLSFVPGVERFGEI
                     TVLDLLSKDSSNMSPEDWKMIAACLRAEEHNYNGFLVLHGTDTMAYSASALSFMLPGF
                     EKPVVLTGSMEPIGVPGSDAEDNIEGAFAFLSALFERKQRGVSIAFHNRLIHGPRSQK
                     ILSHESTAFSSINYPQLGCRENGAIVLEHTPVMHEKYPVNVHSLELETSIFLVTLFPG
                     FRSRYLSHLADAGPKAIVLEALGLGGVPYLGENLLPAIAMCRERNIPVIITTQCVYGG
                     VDLSVYEVGRKTLELGAISGKDMTREAIITKLMAVLPGTDNKQIGNILHCNFCDEIEE
                     PECPADA"
     gene            complement(5542..6069)
                     /locus_tag="PK849_02980"
     CDS             complement(5542..6069)
                     /locus_tag="PK849_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPE65119.1"
                     /translation="MENGIDPRALFTLNYGVYILSTGYEGKYNGQIINALMQLTADPI
                     CMAACLHKDNYTTELVEKSGRFSVSVLEESVPLKFIGVFGFRCGRDFDKFGECRYEVK
                     EGGLPLVTEYSLAGIELKVLSVQDVFTHKVFIGQVEKAQLFKEGTPLTYANYHRVKKG
                     KSPSNAPSAIFNEVK"
     gene            complement(6163..6657)
                     /locus_tag="PK849_02985"
     CDS             complement(6163..6657)
                     /locus_tag="PK849_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPE65120.1"
                     /translation="MISKKIEDAINDQINAELYSAYIYLSMSSWFDSANLKGMSHWMG
                     IQAKEEVEHAMKFAGYVMERGGRVLYKAVEGPETEWTSPLEAFKVAYEHEKYVTRRIN
                     DLMDLAVAEKDYASQALLQWFVSEQVEEEANADEIVSKLEMIGEGKHGLLMIDRELGG
                     REDD"
     gene            complement(6660..7022)
                     /locus_tag="PK849_02990"
     CDS             complement(6660..7022)
                     /locus_tag="PK849_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPE65121.1"
                     /translation="MKKLDIFKCELCGNVVELLHVGGGDLVCCGQPMKLLDEQSADSA
                     TEKHVPVIEGNKVKVGSVPHPMTEQHYIEFIEIMAGDKLCRKFLNPGEAAEAVFEGFV
                     PEKSREYCNIHGLWKAEK"
     gene            7270..>8086
                     /locus_tag="PK849_02995"
     CDS             7270..>8086
                     /locus_tag="PK849_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65122.1"
                     /translation="MQSQSSIIDRFFFVLLLIIVMYACQSEAASSRHIVLLPTLNYTE
                     YEIWESKYYPVNVLEQKMTEYLASLLRQNPFTDVSILDSSQAQRWMDDPYSRQGAFAV
                     QMELYSVLAKEREVLGSFEKGDVSLRVRIYSALDAELETSYISSGKDQRFTFNPGDDR
                     LYLLNTRTSIIDIFYRNGLDFLRLAPADKGQKMSRLTWQQFSGTPYWQAFKNAIKGVA
                     AGIADMGDGELAVIGRIIAPTANSTRRNREYIITLGRQGALAVGDILQVVRGDT"
BASE COUNT         1881 a         2213 c         1885 g         2107 t
ORIGIN      
        1 cgactctttc actctccggg caactgagac cccgtcttca cccggcatcc tgatatccaa
       61 aagcgccacg tcaggacgta tctccgaaat gagcttccac gcggtctcgc cgtcttcagc
      121 ctcccccacc agctccacgg aggtttcctg tgcaagatag gccgatatgc cggctcgcgt
      181 cagcggatga tcgtcagcaa gaaagactcg tacaggcata ctatccactc ctttttcccg
      241 gtcagggagt ttttattgtc cgtaaagcat ggtatcaaga caacaagagg aaaacatcgg
      301 caaagacgcc gaaaaaacag tatctcaggc tcccgccgct gatccgagcc ccaaatccgg
      361 ttcgatacgt ctcatggcca ggatgaccca ttctatgacg gtatccaaag gcattggaat
      421 catctcggct cctttgacta tcagttcacg atccacgcct ttcgcaaagg ctttatcctt
      481 ccacttcttc ttcaccgact tgacctcaag atcctgtact gaacggcttg ggcgaaccaa
      541 tgccgcggtc atcacaagac cggtgagttc gtcgatcgtg aaaaggactt tttccatatc
      601 gttttcaggt ttcacatccg agcagatgcc ccatccgtga gcaagaactg ctctcacgat
      661 atcctcggga tacccttctt cagaaagcca gcgcgccccc tcaatggtat gccgttccgg
      721 cgtttcctgc atctcttccc agtcgatgtc gtgcagaaga cccgccatac cccatttttc
      781 tacatctccg cccatcttct cggcaaagaa tttcatagag gattccacgg caagggcatg
      841 cttgatgtga ccttcatcct tgttgtgttt tttcaaaaga tccagtgcat tttctcgtgt
      901 gaaattcatt ctgtttcgtt cctcctcgca ttttccgccg gcgaaaaaag atccccaaga
      961 agagccccgg cggtagcaaa gaagtccggg gaaaggcatg tcacttcgat agacgcctga
     1021 gacctcatct ctcttgcaag agtatctggg taggggtgaa gaaaaatcac ccgttcaata
     1081 ccggcgttaa taatcgcttt tgtacaaaag gagcagggtt catgcgtgca gtatatctgc
     1141 ccgcccgcag tacatacgcc tgcagctgct gcctgagcta tcgcattcat ctccgcgtgg
     1201 gatcctctgc acatttcatg gcgttctccc gacggaatag acaacaaggt gcggagacac
     1261 cctgcgtcgg cgcaatgcgg aaggcctttc ggcgccccat tgtaccctgt gctgacgatc
     1321 tgcctgtctc tgacaacgac cgcgccgact ctgcggcgaa gacaggtgct ccttgttcca
     1381 gcaacgctcg ccatcatcat gaaatagaca tcccactccg gtcttgaaaa gctcataacc
     1441 cgcccccttg aactgttttt tcgattataa cactcgcaga acatctctat gggatgaaag
     1501 ggacggaagt gtagctgaag aaagcatgat atgatactct ctacggatca caatccgcat
     1561 cttaaccatg gagaggagtt ccatatgttc tcttctttcc cgaaactcga aaaaaaggtg
     1621 tggtcgtggt gcacgtacga tgtcggaaac tcggcgtttg ccacgacaat aatggcggct
     1681 atctatccgg tctattttcg cgaagttgcg tcccgggggc ttgcaccgaa tatctcaacc
     1741 gcctattggg catatacggc ttcggcggcg ctctttctca gcgcattttc ttcgcctctg
     1801 ctcggggccc ttgcagatat ccagggttca aaaaagcgcc ttctcgcgat tttcacagga
     1861 atcggcgtac tctcgaccgg atgcatggcg ttcatcggtc cgggcgattg gcttcttgca
     1921 ctgatcctta tggcggctgg aacaataggg ttcacaatat caattatttt ttacgacgcg
     1981 cttctcccct cgcttgtccc tccagaacgt gtcgatagag tatccgcgca agggtatgca
     2041 tttggatatc tcggaggagg cgtcctgctc gccattaact ttttgtttat tgcgctcctg
     2101 cccggtacaa tcggagctcg cctctctttt ctctccgtcg ccttctggtg gggagggttt
     2161 tctctaccag tgctctttaa tgtagccgaa cctcctgtgg acggagagag agtcgacacg
     2221 aacacggcgt ttcggggcag cgtaaaaagg ctgcaaaaaa ccttcaggga aattcgctcg
     2281 ttccgtaatc tttttctctt cctcgtagcc ttctggttct ataacgacgg tatcggaacg
     2341 atcattcgca tggcggcgat ttacggctct cagcttggaa tttcaatgaa tcatctcgtt
     2401 ggagcgctgc ttcttaccca gtttgtaggt atcccgtttt cgctcgcctt cggcactctg
     2461 gcctcgcaca tggggagcaa aaaaaccatt ctcatcgggc tctatttcta cctctgtctt
     2521 atcgttgccg ctgtctttat cagtaaacca tggcactttt gggcgcttgc tttctcggtg
     2581 gggatggttc agggaggcac gcaagccatt agcagaagtt ttttcgcttc gatgatacct
     2641 ccaggacgta cagccgagtt ctttggtttc tatgatattt ccagcaagtt ctccggcatt
     2701 ctcggtccgg caattttcgg cttcattacc caggtttcag gctcaagtcc gctggcgatc
     2761 gccattctgt cgtacacatt tattctcggt attgtgattc tgcggaagat agaaggatga
     2821 ttttcaatat gcatacttct tttctacgaa ctctctgtct cattctcatt cttcttgttt
     2881 cgttgaatca aaaccgggcc gaagcctccc cccccgtcta ctcgggaagc aggatggtcc
     2941 atacaatcgc aataaccttc gacgacgggc cgcatcaaat tcttacgccg gcactccttg
     3001 acacgctcga tgcgcttgat gtaaaagcca gttttttcct tgtaggaaag atggtggacc
     3061 gttacccggc gctcgtgcgt gaaatatatg cacgcggaca taccgtcgca aatcactcct
     3121 actggcacaa caattcttcg cttctctctc ctgaggaatt cctggatgat cttcgacgat
     3181 gcagcgagtc gatcgaacga aacataccgg ttcgggtacg ttttttccgg cctccgggag
     3241 gcaactatac gaaaaatgtt ctttctctgg tcgaagaagc cggaatgcga actgtgctct
     3301 gggatatcaa cagccgggac tacactggtg tttcggccgc caggatgacg gcaagaattc
     3361 tctcgaaagt gtctccgggt tccatccttc ttttccattc cggagtatcc tcgacgatca
     3421 acgcgcttcc cgagatcgtc tcctcgctcc gaagcaaggg cttcgatttc gtcaccttgg
     3481 acgaaatgct ctcggggtac tccgcgctcc tccctcacac cacgggaagc gagtcttcaa
     3541 cactcgcaag caagtaatgt aaacggtatc caaaggagat gagaaaatgg catcgtttct
     3601 tgtgatttcg actggcggta caatagcctc acgggcaggc tccaacggtc tagtaccttc
     3661 gttgccgggg gaggaacttc tttcgtttgt tcccggggtt gaacgtttcg gggagatcac
     3721 agttctcgat cttctttcaa aggacagttc aaacatgtcc cctgaagact ggaagatgat
     3781 cgccgcatgt ctcagagcgg aggagcacaa ttacaatgga tttcttgtcc tccacgggac
     3841 ggacaccatg gcgtattctg cctcggccct ctcttttatg cttcccggct tcgaaaagcc
     3901 cgtagtactg acgggttcca tggaacctat cggggttccg ggctctgacg ccgaagacaa
     3961 catagaaggc gcttttgcat ttctctccgc tctctttgaa cgaaaacaaa ggggagtctc
     4021 catagccttt cacaacagac tgatccacgg gccccgctcc cagaagattc tcagccatga
     4081 atccactgct ttttcaagta tcaattaccc ccagctcgga tgccgggaaa acggagcgat
     4141 cgttttggaa cacactcctg ttatgcacga gaaatacccg gtaaatgtac actctcttga
     4201 actggagacc tcgatttttc ttgtaactct ctttcccggt tttcgttccc ggtacctctc
     4261 tcatcttgcc gacgccgggc caaaggccat agttctcgaa gcgctcggcc tgggaggagt
     4321 tccgtatctg ggtgagaatc tgctcccggc catcgctatg tgccgcgagc gaaacatccc
     4381 ggtcattatt acgacgcagt gcgtttacgg aggagtcgat ctcagtgtgt acgaagtcgg
     4441 aagaaaaacg ctcgaactgg gcgccatctc cggaaaggac atgacccggg aggcaatcat
     4501 cacgaaactg atggctgttt tacccgggac cgacaacaag cagatcggga atatccttca
     4561 ctgtaatttt tgcgatgaaa tagaagaacc ggaatgcccg gcggatgcgt agtttaacgc
     4621 tccctggcat ttatcaagaa aggagggttc gctcagctcc ttccgtatac cgaatctcgt
     4681 aaggacgaaa tcaaattttg taggatcctc cggaagaatc tcccgaaaaa gtgcggttac
     4741 ttcaatcgca gttttcagat ccggttgttt gcgtcgtgtc attccaaggg ttgagcagat
     4801 ccggaacata tgtacgtcca ttggaataag gagatcccgc ggtttcaggg ctgtccatcc
     4861 gccgggatcg acctcgtccc ttcggaccat ccatttgaga aaaagaaaga atcgtttgca
     4921 tgcgctccca tcactcggag aaggcagaag aaaacggctt tcaagggaac tctttcttcg
     4981 tacgttctca acaaaatttg aaattccttc gagaacatct cccgtcctgc tcaaagaaac
     5041 tgcaagaagc tcctctacgc ttccatattc tcgaagaata cgccctaacc cggtcattag
     5101 cgccgcgatc tccgctccgg atgtgaagcg atggcgaaat aattctgttt cacgagcaag
     5161 aacaagaggc gaaacagtct tgaggaattc cgcgggcgat ggaccgagga gttcaagaag
     5221 acgccgggcg cttcgaagga tcatcgcaac acgcccgtac gctatacttg agacgaggag
     5281 cgccgcaacc tccctgtcct cggtacgaga gtaagggtag atagtctcca gaggatcggg
     5341 agaaacaaat tcacgccggt tgtaccgggc gtatgcttct tcgaaacagg gccatattcg
     5401 atgaagagcg ctcgcttttc taattttttc cgtcattaca ctccccgaaa aataaaccag
     5461 gaaccgtgag agagaaaaac ctacttcccg cacctcacag ttccgtggaa aaattaacca
     5521 ttcgttttca gtcttcggcc gtcacttgac ttcgttgaat atcgcggacg gagcattcga
     5581 tggagattta ccttttttca ctctatggta gttcgcgtag gtcagtgggg taccttcttt
     5641 aaaaagctgc gctttctcca cctggccgat gaagacctta tgtgtgaaga catcttgtac
     5701 ggagagaact ttcagttcta tgcccgccag agaatactcc gttacaagag gaagaccgcc
     5761 ctctttcact tcgtacctgc attccccgaa tttatcgaaa tcgcgtccgc aacggaaacc
     5821 gaagacgccg atgaacttca gagggactga ttcttcgagc acggagacgg agaaacgccc
     5881 gctcttctcc acgagctccg ttgtgtagtt atccttgtga aggcaagctg ccatgcagat
     5941 cgggtcagcg gtaagctgca taagcgcgtt gatgatctgt ccgttgtatt tgccttcata
     6001 cccggtactg agaatgtaca ctccataatt caatgtaaaa agagcccggg ggtcgatccc
     6061 gttttccatt gagtatcctc ccttcatctt cctgcactgc gggcaggagc atgaagctcc
     6121 tgcccgcaga ttgtcttcac taaggagatg cttaaaggac tcctagtcgt cctctctgcc
     6181 acccaattct ctgtcgatca tcaggaggcc gtgcttccct tcgccaatca tctcaagctt
     6241 gctcacgatt tcgtccgcgt tcgcctcttc ttcaacctgc tccgagacaa accattggag
     6301 aagcgcctgg cttgcataat ccttctcggc cacggcgaga tccatgaggt cgttgatccg
     6361 gcgagtcaca tacttctcat gttcgtacgc aactttaaag gcttccaaag gagaagtcca
     6421 ctcggtttcc ggtccctcca cagctttata caggactctt ccgccgcgct ccattacata
     6481 gccggcgaat ttcatcgcat gctctacttc ttccttcgcc tggattccca tccagtgaga
     6541 cattcccttc aaattcgccg aatcgaacca agacgacatc gaaaggtaga tatacgcaga
     6601 gtaaagttcc gcattgatct gatcgtttat cgcgtcttca atttttttac tgatcatgct
     6661 tacttttccg ccttccataa accatgtata ttgcagtatt ccctgctctt ctcaggaaca
     6721 aatccctcaa aaacagcctc tgcggcttca cccgggttca ggaactttct gcatagtttg
     6781 tctcctgcca taatttcaat gaactcaata tagtgctgct cagtcattgg atgaggaacg
     6841 ctgccaacct tcaccttgtt accttcgatg accggcacgt gtttctccgt tgcagaatcc
     6901 gccgattgct catcaaggag cttcatgggc tgaccgcaac agacaaggtc gccccctccg
     6961 acgtgaagca gttccacgac attgccgcac agctcacact taaagatatc cagctttttc
     7021 attcagcatc tttcctcctt tgaaatcgtt ttttttcttg aaaacagatt tattatatca
     7081 ccttccaagt caattctcat cctctccacc cttgaatcag aatatgctct ataataggaa
     7141 gcgggaagaa gacggagagc gcatttctac gaatgcctcc ttccttggct gtccctttac
     7201 cggtagtatc ttctgccgat acattattga gagcagcctg gactccattt gaaaagagga
     7261 tgagaaccaa tgcagtctca atcatcaata atagaccgtt ttttcttcgt tttactgctg
     7321 attatcgtga tgtacgcatg tcaatccgaa gccgcttcgt cacgtcatat cgttttgctt
     7381 cccacgttga attatacgga gtatgaaatt tgggagagta agtactaccc agtgaatgtg
     7441 cttgaacaga agatgactga gtatctggcc tctctcctgc ggcaaaaccc ctttacggat
     7501 gtttcgatac tggatagctc gcaggcgcaa cgctggatgg atgatcctta ctccaggcaa
     7561 ggcgctttcg cagtgcaaat ggaactctac tcggtactcg caaaggagcg cgaggttctc
     7621 ggctcttttg aaaaaggtga tgtgtccttg cgagtcagaa tttacagcgc tcttgatgcg
     7681 gagcttgaaa cttcctatat ttcctcgggg aaagaccagc gattcacgtt caatccaggc
     7741 gacgacaggt tgtatctgct gaacacacga acgagcatta tcgatatttt ctatagaaac
     7801 ggccttgatt tccttcgact tgccccggca gataaggggc agaaaatgag tcgtcttaca
     7861 tggcagcagt tttccggcac cccctactgg caggcgttta aaaacgctat caaaggtgtg
     7921 gctgcaggaa tagccgatat gggggacggc gaactcgctg ttatagggcg cattattgcc
     7981 cctacggcaa actcgacacg acgcaacaga gagtacataa taactctcgg tcgacagggc
     8041 gctctcgctg tcggcgacat acttcaggta gtcagaggcg atacat
//
LOCUS       DAOPKU010000060         8049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9500_length_8049_cov_4.710158, whole genome shotgun sequence.
ACCESSION   DAOPKU010000060 DAOPKU010000000
VERSION     DAOPKU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9500_length_8049_cov_4.710158"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1175
                     /gene="moaA"
                     /locus_tag="PK849_03000"
     CDS             117..1175
                     /gene="moaA"
                     /locus_tag="PK849_03000"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPE65123.1"
                     /translation="MGRIMRDPYGRTLDYVRISITDRCNFRCLYCMPANGIAWIPHED
                     IMTYEDIFFLVSVLSELGVKRLRFTGGEPFVRKGFVSFLQEIRQRFPSIDVAVTTNGA
                     FLERDASALSALRLHSVNVSLDTLHPQKFADITRTGELGTVLRGIRALVAKGGVSVKI
                     NTVVMRDFNLNEISGLVRFAKENDVLLRFIEFMPLDKDVWSNQQYVSASEILSLLPDA
                     LQWSPSPKSSVTTQNTSSAIPNGPAKYFRNAKTGQDIGIISAVSDHFCHTCNRLRITS
                     TGEVRPCLFSNRGVAIIDALRRKDPDAARDGILMAVKNKPHEGGTGIPEERQNPEDAP
                     QKRTDLFQEERHMSRIGG"
     gene            1183..1707
                     /gene="moaC"
                     /locus_tag="PK849_03005"
     CDS             1183..1707
                     /gene="moaC"
                     /locus_tag="PK849_03005"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPE65124.1"
                     /translation="MSIIEDEKNELTHLDEQGRPLMVDVGEKPFSARTAEAEGWVLLD
                     QAICDLLGDKGLSKKGDVLRIAETAGIMAVKKTWELIPLCHSIRVDSVRVQCELLADI
                     RKIHIKSIVKANDVTGVEMEALTGVSVAALTVYDMCKAVSKGMVIDGIRLLRKTGGKS
                     GDYIASTPIQVEDV"
     gene            1802..2536
                     /locus_tag="PK849_03010"
     CDS             1802..2536
                     /locus_tag="PK849_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPE65125.1"
                     /translation="MNGTEKPFFLLSPGEREKLPEKSVGFVLSKDALKFSYFLSSGED
                     VLFRILEGNPLPRLSVEQGGFISVSHRMQLLRPISVGILTVSDKGSRGEREDLSGPAL
                     ARSVKPLGMFVEEVAIVPDEIGIISDTLTKWSDTGRLNLILTTGGTGLSKRDVTPEAL
                     LSVAEKHVPGIGETMRSVSMKITSKAMLSRGCAVIRGETLIIALPGSEKAAEECFAAI
                     APALRHGIEILCGWDSECGSRPHHHH"
     gene            2604..3485
                     /gene="rapZ"
                     /locus_tag="PK849_03015"
     CDS             2604..3485
                     /gene="rapZ"
                     /locus_tag="PK849_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPE65126.1"
                     /translation="MDAVGVNKCIIITGMSGAGKSTALKILEDQGMFAIDNIPPVLLS
                     QLLFLLEKHRAAVRWGVAAVVDIRGDSLLDDFPAALALLRKQTRHVHILFFDAKDEIL
                     LRRFETTRRRHPLEPDGSFLDGIAKERERLQPILDYADTIIDTSALSLSAIRKELISR
                     FFEDAAAVPLVISSFGFKHGAPADCDYVFDVRFLDNPFYNPVLKPLSGKDEPVQEEIW
                     RSVEARGFWEKLISFLEYIVPLYLRSGKAHLHIGVGCTGGRHRSVAVAERLYAHFKEY
                     SGGCILRHRDIEKDQGW"
     gene            3488..4609
                     /gene="yvcK"
                     /locus_tag="PK849_03020"
     CDS             3488..4609
                     /gene="yvcK"
                     /locus_tag="PK849_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HPE65127.1"
                     /translation="MDLLISYGLGLFTAIILVFLFRLRYSGRTGQLLQRAPQERSTAI
                     SKAIEYRLSMGPCVAALGGGTGLSTLLRGLKSFTRNIVAVVTVTDEGGSSGKLREEWG
                     VLPPGDVRNCIVALAENDNALKRLLDFRFDRGALAGHSLGNLMLLAISEQFGDFRLAV
                     EEMNHLLAIRGRVLPVTTESVSIVAETRDGSRIRGELSVSEHGSQIHKIWLEPRDAKP
                     LKEVLLSLDEADIIVLGPGSLFTSIIPNLLIRKVAERLRQSEAPKVYIANLMTQPQET
                     QGFNILDHVRWISAAMGVSPDYVLVNEGVIPHLLEEKYRVEGAEPLYITEEEEWELEK
                     MGCKILRGDFVRIMDGNVVRHNGQALSEILIRLCRELDE"
     gene            4609..5508
                     /gene="whiA"
                     /locus_tag="PK849_03025"
     CDS             4609..5508
                     /gene="whiA"
                     /locus_tag="PK849_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HPE65128.1"
                     /translation="MKPIDSRSTLTSSMWDEWTSSIASPGRAVEDEIRGIVSGLSEVA
                     SHERETVFSSGRLWIFRRLRMLWSAWENARNYEFASLLNIPRSMKGRVFFRLPSEIYA
                     TFRERESESRNTPWLRGLWGSCGSLYLPKTGYYLCFRISSRITEEKTAHLLQRNGFTA
                     GRRKIQGKYEVTLRNQQEIADILASFNMVKTMLHLEEKAIFRSLRNQANKLVNCDASN
                     IRKSLEASSRQIEISRNALELPDYDDFPAVLKDLISTRLANPSMTLEELGRIQSPPVS
                     KSTIKYRWKKIEDLATPLWQRRK"
     gene            5606..6793
                     /locus_tag="PK849_03030"
     CDS             5606..6793
                     /locus_tag="PK849_03030"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPE65129.1"
                     /translation="MKLRTFSESDVRGKNVLVRVDFNVPLKNGKVTDSTRILAHRETV
                     STLLGWGAQVALSSHLGRPKGKRVPEMSLECVLSEAEKAFRVPTRFCNDCIGPSVQSA
                     VDGMKAGELLLLENLRFYAEEQENDTAFAGKLAAPFDVFVMDAFSASHRADASTNAIM
                     GILPSFSGALLRREVEMLSAVSEKPESPFVLILGGAKVSDKIGVVEHLMDKTSTILIG
                     GGMAFTFLKAAGKPIGRSLFEAEKKDFALEMMEKAQRSGVEILLPTDVVVGTSLEMTS
                     GEKIVSADALPDDAMGLDIGPKTVSLFAERIAGARTVLWNGPMGVFENPLFASGTKGV
                     AEGVAACTRKGGITVVGGGDTAAAAKMLGFDQNVSHVSTGGGASLEFCEGKDLPGIKP
                     LEE"
     gene            6821..7615
                     /gene="tpiA"
                     /locus_tag="PK849_03035"
     CDS             6821..7615
                     /gene="tpiA"
                     /locus_tag="PK849_03035"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPE65130.1"
                     /translation="MKKIYLYGNWKMNMLPSEAEAFSNELLSETAGELYSTRSVDIAL
                     FPPYISIAPVVRMLPEKSFIKAGAQDGWFEDRGAYTGAVSMDMIREIGCTHVLVGHSE
                     RRSIFGDTDEIVGRKLKQAIQKGLIPVLCFGETLEQREAGQTLAILGKQLLAAWKDIE
                     ADEIKGSDNLPGILLAYEPVWAIGTGRNAKAADAQEACAFSRNLFKERFGKQMDCPVL
                     YGGSVKESNAAELLSQQDIDGALIGGASLKVGSFLGIYENYRKSAD"
     gene            complement(7749..>8049)
                     /gene="rsfS"
                     /locus_tag="PK849_03040"
     CDS             complement(7749..>8049)
                     /gene="rsfS"
                     /locus_tag="PK849_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPE65131.1"
                     /translation="KRGLDIVALDLEGSSAISDVFILVTANSDVHMDTLRDAALEALH
                     VQGIKTTVEGNDSMRWRLIDAGDLTVHVFSKAGRDHYNLEKMWRDARSYHYAYHD"
BASE COUNT         2032 a         1978 c         2135 g         1904 t
ORIGIN      
        1 ccctatgaac tcgtctactt gaaagaattc gaaagcctta caggactgaa agccgtgctc
       61 aacgcattgg acgataagga gtggcgcgcc ggcgtactgc gtatgggagg ataccgatgg
      121 gcagaataat gcgtgatcct tatggaagaa cgcttgacta cgttcgtatt tctatcaccg
      181 accgctgtaa ttttcgctgt ctttactgca tgccggcaaa cggtattgcc tggattcctc
      241 atgaagatat tatgacgtac gaagacatat tctttctcgt ctccgttctt tctgaacttg
      301 gagtaaagag gcttcgtttc accggcggag agccttttgt caggaaggga ttcgtttcgt
      361 ttctgcaaga aatacggcaa agattccctt ccatcgacgt cgcggtaacg acaaacggag
      421 cgtttctgga acgggatgca tcagcacttt ctgctctccg ccttcattcg gtgaatgtga
      481 gtcttgatac gttgcacccg cagaagttcg ctgacattac gaggacgggg gaactcggaa
      541 cagtgctccg cggaattcgc gccctcgttg caaagggagg agtttctgta aagataaaca
      601 cagtggtaat gcgtgatttc aacctcaatg agatttccgg gctcgttcgc ttcgccaaag
      661 aaaacgatgt actgctcagg tttatcgaat tcatgccgct tgacaaggac gtctggtcaa
      721 accagcagta cgtctccgcc tcggagattc tctcgcttct cccggacgct cttcagtggt
      781 ccccctcacc gaagagctct gtgacaacgc agaacacaag ctctgcaatt cctaacggcc
      841 cagcaaagta tttcaggaat gcgaaaacag ggcaggatat cggtatcatc tcggctgtct
      901 ccgatcattt ctgccatacg tgcaatcgcc ttcggataac atcaactggt gaagtgcgcc
      961 cttgcctctt cagcaacaga ggcgtcgcca tcatcgacgc tttgcgaagg aaggatcccg
     1021 acgccgcccg cgatggcatt ctcatggcgg taaaaaataa accccatgaa gggggtacgg
     1081 gtattcccga agagcggcaa aacccggaag acgctcctca aaagcgtaca gatctcttcc
     1141 aagaagaacg tcacatgtcc cggatcggag gatgaactct ctgtgagcat tattgaagat
     1201 gaaaaaaatg aattgacgca tctggatgaa caggggcggc ctctaatggt cgatgtcggc
     1261 gagaagccct tttccgcccg cacagcggag gcggaaggat gggttttgtt ggatcaggca
     1321 atctgtgatc tcttgggcga caaaggcctc tcaaaaaaag gagatgtcct tcgtattgcg
     1381 gaaactgccg gaataatggc cgtgaaaaaa acgtgggagc tgatcccttt atgtcactcg
     1441 attcgagtcg acagtgtacg agtgcagtgc gagctgctgg cggacatccg aaaaatccat
     1501 atcaagagca tagtgaaggc aaatgacgtt acgggagttg aaatggaggc gcttaccggc
     1561 gtttcagtag cggctctcac ggtgtacgat atgtgcaaag cggtctcgaa agggatggtt
     1621 attgacggca tccgtcttct ccgtaaaacc ggaggaaaaa gcggcgacta catcgcttcg
     1681 actccaattc aagtggagga cgtttgacat gaaagtcctc gctattattg aaaggtctac
     1741 ccgtaccttt ctcccgcaca cgctcagtta cgtaagcatt aacaaagccg gagaaagcgc
     1801 cttgaacggg acagaaaagc cgttctttct gctctctccg ggagagagag aaaaacttcc
     1861 ggaaaagagc gtcggctttg ttctctccaa agacgcgcta aaattctcct atttcctctc
     1921 ttccggagag gatgttctct ttcgcatatt ggaagggaat ccgttgccgc gattgagcgt
     1981 tgaacaggga ggtttcatct ccgtttcaca tagaatgcag ctgctccgtc cgatttcggt
     2041 cggcatactc accgtgagcg acaaagggag caggggagag agggaggacc tctccggtcc
     2101 ggctctagcc cgtagcgtca agccgctcgg gatgtttgtc gaagaagttg cgattgtccc
     2161 tgatgagatc ggaatcatct cggatacgct cacgaagtgg agcgataccg gccgtctcaa
     2221 tctcatactg actacaggcg gaaccggtct gtccaagaga gatgttactc ccgaggcgct
     2281 tctttccgtt gccgaaaaac atgttccggg catcggggaa acaatgcgca gcgtttcgat
     2341 gaagataacc tcaaaagcca tgctgagcag aggctgcgcc gttatccgtg gagaaacgct
     2401 tattattgcc cttccaggga gcgaaaaggc tgcggaagaa tgcttcgctg caatagctcc
     2461 cgcactgcgc cacggtatag agatactgtg cggatgggac agtgaatgcg gctcccgccc
     2521 gcatcatcat cattgagcag cccgataaca gagaaaatat acattggggc gcaaatctgt
     2581 atgaagggag acgaagcgtg gatatggatg ccgtcggtgt gaacaagtgc attattatca
     2641 cgggtatgtc cggcgcaggg aaatccaccg cgctgaaaat cctcgaagat caaggtatgt
     2701 ttgcaattga caacatacct cctgttctgc tttctcaact gctctttctt ctggaaaaac
     2761 atcgggcagc cgttcgctgg ggagtcgcag cggtcgtgga tattcgcgga gattcgctgc
     2821 tggatgattt tccggcggcc ctcgccttgc tgagaaaaca gacgcgtcat gtgcatattc
     2881 tctttttcga tgccaaggat gaaattttgc tgcggcgttt tgaaaccaca cggcgccgcc
     2941 atcctctgga acccgacggg tcttttcttg acggtatcgc aaaggaacgc gaacgcctac
     3001 aaccgattct cgactacgcc gatacaatca tcgatacctc ggcgctctct ctttctgcaa
     3061 ttcggaaaga gctgatctcc aggttttttg aggatgccgc ggccgttccg cttgtgatct
     3121 catccttcgg tttcaaacac ggagctcctg ctgattgcga ctacgttttc gacgttcgtt
     3181 tcctggataa tcccttctac aatccggttc tcaaaccgct ttcggggaaa gacgaacctg
     3241 ttcaagagga gatctggcgt tcagttgagg ctcgcggttt ttgggagaag ctcatttctt
     3301 ttctcgaata tatcgtccct ctgtatctcc gttcagggaa agcgcattta catataggcg
     3361 tcggctgcac aggaggacga catcgctcag tggccgttgc ggaacgtctc tacgctcatt
     3421 tcaaagagta ttcaggcgga tgcattcttc gtcaccggga tattgaaaaa gatcaggggt
     3481 ggtgattatg gacctgttga taagctatgg actcggtctt tttaccgcca taatcctcgt
     3541 ctttcttttt cgtttgagat actccgggcg taccggacag cttcttcagc gagcgccgca
     3601 agagcgcagc acagccatct cgaaagctat cgagtatcgc ctttcgatgg gaccttgtgt
     3661 tgcggcgctc ggcggaggta ccgggctttc aacccttctg agagggctaa aaagctttac
     3721 acgcaatatc gtggcggttg ttacagtaac tgacgaagga ggcagttcgg gaaaactccg
     3781 cgaagagtgg ggagtcctcc ctccgggcga cgtccgtaac tgcatagttg ctcttgcaga
     3841 aaacgacaat gcgctgaagc gccttctcga ctttcgattt gatcgcggcg ccttagcagg
     3901 gcacagcctc gggaacctga tgctgctcgc tatctccgag cagttcggcg acttccgcct
     3961 cgcggtagaa gaaatgaatc atctgctcgc tattcgcggc agagtcctcc cggtaactac
     4021 ggaatccgta agtatcgtgg ctgaaacacg ggacggttcg cgcatccgag gggagctctc
     4081 tgtgtccgaa catggatctc agattcacaa aatctggctt gaaccaagag acgccaagcc
     4141 gctcaaggaa gtgctgcttt cgcttgatga ggccgacatc atcgttctgg gtccgggaag
     4201 cctctttacc agtatcattc cgaacctcct cattcgaaag gttgcagaaa ggctgcgcca
     4261 gagtgaagcc ccaaaagtct atattgccaa tctcatgacg caacctcagg aaacccaagg
     4321 attcaacata cttgatcacg tccgctggat cagcgcggct atgggggtat ctccggatta
     4381 cgtcctcgtg aacgagggtg tgattcctca tcttctcgaa gaaaaatacc gtgtagaggg
     4441 tgcggaacct ctttatatta ctgaagaaga ggagtgggag ctggaaaaaa tggggtgcaa
     4501 gatactacga ggagactttg ttcgaatcat ggacggtaat gtagtgcgac ataacggaca
     4561 agcgctctcc gagattctga tacgactgtg ccgggaactg gatgagtgat gaaaccgata
     4621 gattcccgct caacgctgac gtcaagcatg tgggacgaat ggacatcgtc aatagcctcc
     4681 cccggtcgtg ctgtcgagga tgaaatcaga gggatagtgt ccggtctttc agaggtggca
     4741 tctcatgaaa gggaaactgt tttttcgagt ggacgccttt ggatttttcg acgcctcaga
     4801 atgctctggt ctgcatggga aaacgcacgg aattatgaat tcgcttcgct cctcaacatc
     4861 ccgcgttcaa tgaaaggacg cgtttttttc cgtcttcctt cagaaattta tgcgacgttc
     4921 cgcgaacgag aatctgaatc tcgaaatacg ccctggctga gggggttgtg gggaagttgc
     4981 ggctctttgt atttgccgaa gacaggctac tatctctgtt ttcgaatctc ctcccggata
     5041 acggaggaga aaactgcaca tcttttgcag aggaacggat ttacagctgg aagaagaaaa
     5101 attcagggga aatatgaagt cactctcaga aaccagcagg agattgcaga catacttgcg
     5161 agtttcaaca tggtaaagac aatgctccat cttgaggaaa aggcgatctt ccgatcgttg
     5221 agaaaccagg caaacaagct ggtgaactgt gacgcctcga atattcggaa gagcctggag
     5281 gcatcgtctc gtcagatcga aatatcgcgc aacgcactcg aactccctga ttacgatgat
     5341 ttcccagccg ttctgaaaga ccttatatcc acacgtctgg ccaatccaag tatgacgttg
     5401 gaagaactcg gccgtatcca gtctccccct gtaagcaaaa gtactataaa atatcgctgg
     5461 aaaaaaatag aagacctggc aaccccttta tggcagcggc gaaaataaaa tttctctaac
     5521 ttcagatccc gcgatgccaa agtgaagggg tacgccatat aatagaaatg ctcacgacaa
     5581 agaagctaaa ggaggtcgga aacacatgaa actgcgtacg ttttcagaaa gcgatgtccg
     5641 tgggaaaaat gtgcttgtgc gggttgattt caatgttccg ttgaaaaatg ggaaagtaac
     5701 ggattccacg cgaatccttg cccacaggga aactgtttct acgcttctgg gatggggagc
     5761 ccaggttgcg ctctcttccc atcttggccg accaaaaggg aaaagagttc ccgaaatgtc
     5821 tctcgaatgc gttttgtcgg aagcggaaaa ggcgttccgt gttccaacca gattttgcaa
     5881 cgactgcatt ggaccttcag tccaatctgc agttgacgga atgaaagcgg gggagttgct
     5941 cctgctcgaa aaccttcgtt tttacgcgga agaacaagaa aatgacacgg cgttcgccgg
     6001 caagctcgcc gctccctttg atgtcttcgt catggacgcg ttcagcgcct cgcacagagc
     6061 agacgcgtcg acaaacgcta ttatggggat tctcccctcc ttttcaggcg ctctgcttcg
     6121 tagagaagtg gaaatgctgt cagccgtgag cgagaagcct gaatctccgt tcgtccttat
     6181 cctcggaggc gcaaaggttt cggataaaat cggcgtggtc gagcatctca tggataagac
     6241 aagtacgata ctcatcggag gcggtatggc cttcacgttc cttaaagcag ccgggaaacc
     6301 gattggacgg tcccttttcg aggcggaaaa gaaagacttt gccctcgaga tgatggagaa
     6361 ggcacagcgc tcaggagtcg aaatcctctt accgacagac gttgttgtag gaacctctct
     6421 agaaatgacg agcggtgaaa agattgtgtc tgccgacgct cttcctgacg atgccatggg
     6481 attggatatc ggaccgaaga ccgtatccct tttcgccgaa agaatcgcag gagccagaac
     6541 tgttctctgg aacgggccga tgggcgtctt cgaaaatcct ctctttgcct cgggaacgaa
     6601 gggggtggct gaaggagtgg ccgcatgcac ccgcaaaggt ggcattaccg tcgtcggagg
     6661 aggagatact gccgcagcgg caaaaatgct cggcttcgac cagaatgtct ctcacgtttc
     6721 aacaggaggc ggggcaagtc ttgagttttg cgaagggaaa gaccttccgg gaatcaaacc
     6781 gctcgaagag taaacaataa aggaggccga tgagcatacc atgaaaaaaa tctatcttta
     6841 tggcaactgg aagatgaaca tgcttccttc cgaagcggaa gctttttcaa acgaacttct
     6901 ttctgaaaca gcgggggaac tgtatagcac acgttcggtc gacatcgctc ttttcccgcc
     6961 ctacatatcg atagcgccag tagttcgcat gctcccggag aaaagcttca tcaaagcggg
     7021 cgctcaggac ggctggttcg aagaccgggg cgcctacacc ggtgcagtat ccatggatat
     7081 gattcgtgaa atagggtgca cgcatgtact tgtgggtcat agtgaaagaa ggagcatatt
     7141 cggcgataca gatgagatcg tcgggcgaaa actgaaacag gccatccaga aaggacttat
     7201 acccgtactt tgtttcggag agacgcttga acagcgtgaa gctggacaaa cgctcgcaat
     7261 tcttgggaag cagcttctgg cggcatggaa ggatatcgaa gcggacgaaa tcaaaggttc
     7321 ggataacctt cccggtattc tgcttgcata tgaacctgta tgggctatcg gcacaggacg
     7381 taacgcaaag gctgcggatg ctcaggaggc atgtgctttt tcacggaatc ttttcaaaga
     7441 gcgatttggt aagcagatgg attgtcccgt tctgtacgga ggcagcgtca aagaatcaaa
     7501 tgccgcggag cttttatctc agcaggatat cgacggcgct cttatcggag gagcttcttt
     7561 gaaggtcggt tcgttccttg gaatttatga aaactaccgg aagtcggcgg actgaaaaag
     7621 agaatatgta acataagata cattatagga cacctcaaaa agagaactga aaaaccgggc
     7681 agacttcgtt tcggtctgcc cggttctctt ttgaatctct ttatagaaac gaaaaaaacg
     7741 cagcgttctc aatcatggta tgcataatga tacgagcgcg cgtctctcca catcttctct
     7801 aggttatagt gatctctccc ggctttactg aacacatgta ccgtcaggtc tccggcgtca
     7861 ataagacgcc accgcatact gtcgtttccc tcgacggttg tcttgattcc ctgcacgtga
     7921 agcgcctcca gagctgcatc ccgcagcgtg tccatatgta cgtcggaatt tgccgtcacc
     7981 aatatgaaaa catctgaaat cgccgaagag ccttccaggt caagcgccac gatatcaagc
     8041 cctctcttg
//
LOCUS       DAOPKU010000061         8046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9506_length_8046_cov_4.615317, whole genome shotgun sequence.
ACCESSION   DAOPKU010000061 DAOPKU010000000
VERSION     DAOPKU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9506_length_8046_cov_4.615317"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PK849_03045"
     CDS             complement(<1..338)
                     /locus_tag="PK849_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPE65132.1"
                     /translation="MSKVVGIDLGTTNSCIAVQEGDVTTIIPNAEGNRTTPSVVAFTK
                     EGERLVGQLAKRQAIVNGDRTIMSIKREMGTDHRVTVDGKKYTPQEISSMILQKMKRD
                     AEDYLGEPVT"
     gene            complement(486..1091)
                     /locus_tag="PK849_03050"
     CDS             complement(486..1091)
                     /locus_tag="PK849_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPE65133.1"
                     /translation="MSSVNDQVSSELEKEGVASASTEESVSRDEPEARQEVAPSPEEL
                     LAALQNAEREKIELTEALARLRADFFNYRKRVDRDRQKERSMIAEEKALDFLPVLDNL
                     DRALSVSPDIDGKSVLQGVAMVRKQFLAVLESMGVEAVPSIGSVFSPEYHEAVVAVAT
                     DDPEKNGIVMEEIQSGYRTKERVLRPAKVKVASYTLTAEKG"
     gene            complement(1116..2132)
                     /gene="hrcA"
                     /locus_tag="PK849_03055"
     CDS             complement(1116..2132)
                     /gene="hrcA"
                     /locus_tag="PK849_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPE65134.1"
                     /translation="MLTERQLEVVFSVVYEYIQTGEPAGSRTISKKYLRGCSPATIRN
                     EMADLEEMGYFYQPHTSAGRQPTSKAYRLYVDAIMQRRRTAPAETEKWKREIGERKQG
                     IESILTYVSRLLGQATNYVGVAALSALEEVQIQRVSFLRLGGNTVLLLIVLEGGLVHH
                     SNIQLPCELSQDMLDELGRRITTIAEGHSWSEVRDVLFRYVLDGLERVWDTCREAIVQ
                     MDALLNRRSSRLFIGGAQHILSLPDFQDIGKFQTVLSLLEQEQALAEMVERYSVKQGV
                     SVTIGDENTEEDMKQLSLVLVPASGQSRRAILGLIGPLRMDYEKSIAILETLAEDLDD
                     MLVQ"
     gene            2331..2978
                     /locus_tag="PK849_03060"
     CDS             2331..2978
                     /locus_tag="PK849_03060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPE65135.1"
                     /translation="MNEHTVRTTILLVRHGECRGNVDGLFRGRVDFPLNETGLLQAEE
                     VAGALASYRPEAVFSSPLLRALQTAESITAASSSHLFIEDGFNNICLGTWEGRPKKEI
                     AALFPEMWRTWLENPEALSVEGAESLNDVLVRSKKSLDRIVAEYQGKTVVAVTHRTVV
                     KPLLAGCLGIPSPYFWKTHMDTAAYSVLHFDDLHGYSLFCLNQTNHLSRFTTEWI"
     gene            3138..3875
                     /locus_tag="PK849_03065"
     CDS             3138..3875
                     /locus_tag="PK849_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65136.1"
                     /translation="MRDGKLLLRITLVLVLCLLARQSSAYVKILTAPGHPVSLIVEHD
                     EGRIRKAFFRSPEGVRPLEHIEQHLLLSDSLTFLCADDDILDDLVWKIDLLQPKTEKM
                     LSLWITSVTEASTAWLALAPSGAGFWESLPRAPLPREDVFLYIAPHLPAYQELGQRSG
                     PEILTFIYTMLLTKNGPKLAAVPEIYRQFLPLTALVCRAQENKDLKAAYTALHQDFER
                     MGQGGMPSREAIDNFLWKRILTVRWKR"
     gene            complement(3919..4380)
                     /locus_tag="PK849_03070"
     CDS             complement(3919..4380)
                     /locus_tag="PK849_03070"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4255..4257),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPE65137.1"
                     /translation="MEGKPVPGFLLQDGSGKEFSHKDFSGSWLALFFYSKDNTSGUTN
                     EVKDFADRMSAFEELNCRIVGVSPDSVESHRKFAAKLDISFPLLSDPEHEFIEACGFW
                     KQKKMYGKEYMGVERSTLLVTPDGIVARLFSKVKVAGHAEDVLHALKERAG"
     gene            complement(4394..5251)
                     /locus_tag="PK849_03075"
     CDS             complement(4394..5251)
                     /locus_tag="PK849_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE65138.1"
                     /translation="MGTSFDLPGIEISWCPGCGDFKILDSIKQALEELGLSPLDVVVV
                     SGIGQAAKTPHYMKSHFFNGLHGRALPNATAIKAANPSLEVVAVGGDGDMYGEGGNHF
                     LHTIRRNPGITNLVFNNMVYGLTKGQASPTSPRGFRTSVQVDGVHAQPFNPLATALVQ
                     GAGFVARAYAGDVEKTKDVIKEAVQYKGYALVDIFQPCVSFNSVNTYAWFKENTYWLD
                     GHDPSDFSKALSLALSEEPFPLGVLFRGEERTTFEGSLAAYRNDKNPLFRRAAPREAV
                     KKLLDGMCR"
     gene            complement(5251..6231)
                     /locus_tag="PK849_03080"
                     /pseudo
     CDS             complement(5251..6231)
                     /locus_tag="PK849_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
     gene            complement(6253..6993)
                     /locus_tag="PK849_03085"
     CDS             complement(6253..6993)
                     /locus_tag="PK849_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE65139.1"
                     /translation="MIFPFSGEVKEVSCVLSGAAGLGIQTVEDMLARIVVDSGFSVFG
                     SREYMSRVRGGNNSTELRIAPFRVDALVDRMDILVALSRNVRENIRERITPSTLIIGD
                     KEELKGELEDAGALFVDVPLSRLAAEAGGRIYSNSVAAGILLGILGLGTEKAEAFFEE
                     RFSGKKGDVAGKNTTACRSGYELGEKLVKGKGLLSGMQADLQVGKALINGSDAVSLGA
                     MAAGCNFVTAYPMSPATGVLSFFAKHAE"
     gene            complement(7449..>8046)
                     /locus_tag="PK849_03090"
     CDS             complement(7449..>8046)
                     /locus_tag="PK849_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HPE65140.1"
                     /translation="MITGTIAQGGPTITYAQGFGKFAAIIGIDPKVGMSLGLLAINSF
                     LLTSLDTATRLTRYQIQELSNMKIDKYTATVIAVAAGMALLLTKAHGPTGNVIPAWAA
                     IWPIFGASNQLVAALALLTIGVWVGKALKKDNRFAMYPMWFMLVTTVAALGFLIKDNM
                     AYEHPNYILVVPSIILLVLAIMMVFESLKALKNPDIKA"
BASE COUNT         1867 a         2362 c         2006 g         1811 t
ORIGIN      
        1 ttcgtcaccg gctcgccgag atagtcctcc gcgtcgcgct tcatcttttg caggatcatc
       61 gaagagatct cctggggagt atactttttc ccgtcgacgg taactctgtg gtcagttccc
      121 atctcacgct ttatggacat gatcgtcctg tcgccgttca cgatagcctg gcgcttggcg
      181 agctggccga caagacgctc tccttccttc gtaaacgcga cgacggacgg ggttgtgcgg
      241 ttcccttcag cattggggat tattgttgta acgtccccct cctgaacagc gatgcagctg
      301 ttggttgttc caagatcgat tcctaccact ttcgacatat ttttctcacc tcgtaatagt
      361 ttgaacagaa gatcgtactc cgcacaagac atggcaaaag agtgcacacg ctgtaagagc
      421 tcgctctgaa tgtactcggg gggaccgccc cctgcgggaa accggcagag aaagaagcgg
      481 acgcgctacc ccttttccgc cgtaagcgta taagaagcta ccttcacctt cgccggacgg
      541 agcacgcgtt ccttcgtccg ataaccggac tgtatctcct ccatgacgat tccgtttttt
      601 tccgggtcat cagtcgcaac cgcaactact gcttcgtggt actccggcga gaagacagag
      661 cctatagacg ggacagcctc tacgcccatc gattccagaa ctgcgaggaa ctgtttcctg
      721 accatggcga cgccttgaag cacgctcttc ccgtcgatgt ccggggacac tgaaagcgcg
      781 cggtccagat tgtcgaggac cggcagaaaa tcaagcgcct tctcttcggc gatcatcgac
      841 cgctctttct gacggtctct gtcgacccgt ttgcgatagt tgaaaaaatc cgcgcgcaat
      901 ctggccaagg cctccgtcag ttcaattttc tcacgctccg cattctgaag cgcagcaagc
      961 agctcttccg gtgaaggcgc aacctcctga cgagcttccg gttcgtcccg ggagacgctc
     1021 tcttcggttg aagcggaggc gaccccctct ttttccagct ccgaagaaac ctgatcgtta
     1081 acactgctca ttcgtgtcgc tcctttcata taaaactact ggacgagcat atcgtccaaa
     1141 tcttcagcaa gggtttccag tatggcaatc gacttctcgt agtccatacg gagcgggcct
     1201 atcagcccca ggatcgccct gcggctctgg ccggatgccg gcacgagaac aagagagagc
     1261 tgcttcatgt cctcttccgt gttttcatct ccgatggtaa ccgagacccc ctgttttaca
     1321 ctatagcgtt cgaccatctc tgcaagggcc tgttcctgtt caagaagaga gagcaccgtc
     1381 tgaaacttcc ctatatcctg gaaatcgggc aggctcagga tatgctgggc gccccctatg
     1441 aacaggcggg aactccgcct gttcaagagc gcgtccatct ggactattgc ctcgcggcag
     1501 gtatcccaga cccgctccag accgtcgaga acatatctga aaagaacatc gcggacttcg
     1561 ctccaagaat ggccctcggc tatggtggtg atacggcgtc caagctcgtc aagcatgtcc
     1621 tgagaaagct cgcagggaag ctggatgttg gagtgatgca ccagtccgcc ttcaagaaca
     1681 atcagaagca gcacagtatt gcctccaagg cgaaggaagc tcacccgttg gatctgtacc
     1741 tcttcaagcg cagagagagc cgctaccccc acatagttcg tcgcctgccc gaggagccgg
     1801 gaaacatagg tgaggatcga ttcgatcccc tgttttcgtt ctccgatctc ccgcttccac
     1861 ttctccgtct cggcgggagc ggtccgcctt cgctgcatga tcgcatccac atacaggcga
     1921 tacgccttcg acgtaggctg ccttccggcg gacgtatgcg gctggtagaa gtaccccatc
     1981 tcctccaggt cggccatttc atttcgaatt gtcgccggac tgcatccccg gagatacttc
     2041 ttcgaaatcg tccgggagcc cgcgggctct ccggtctgga tatactcgta gacaacggaa
     2101 aaaacaactt cgagctgcct ctccgtaagc atttcgacac ctcctcgttg cgttagcact
     2161 ctttcaatgt gagtgccaat gcatcgctcc cggaaggtag agtaacaatc catgatatga
     2221 ttgtcaagac tggtcaaagg cttttatttt ttttcccgct atcgcggcag atccacgatg
     2281 aagctctctt ccgtcgtcga ctccacgaga ctgcggagac ggagggaaaa atgaacgaac
     2341 acaccgttcg caccaccatc ttactcgtgc gtcacgggga gtgccgagga aatgttgacg
     2401 ggctttttcg gggaagggtt gattttcctt tgaacgaaac cggactcctg caagccgaag
     2461 aagttgccgg cgccctcgct tcttatcggc cggaagcagt cttttcgagt cctcttctca
     2521 gagccttgca gaccgccgaa tcaataaccg cggcgtcatc ttctcacttg tttatagagg
     2581 atgggttcaa caacatctgt ctcgggactt gggaagggcg tcccaagaaa gagatagccg
     2641 cactattccc tgagatgtgg aggacctggc ttgaaaaccc ggaagcgctt tcagtcgagg
     2701 gggcggaatc tttgaacgat gtccttgtgc gctcaaaaaa aagtcttgac cgaatagttg
     2761 ccgaatacca agggaaaacc gtcgtcgcag tcacacacag aactgttgtc aaaccgcttc
     2821 tcgcaggatg cctcggtatt ccttctccct acttctggaa aacccatatg gataccgctg
     2881 cctactcagt gctccatttc gacgatcttc acgggtatag cctcttttgt ttaaaccaaa
     2941 cgaatcatct ttcacgtttt actaccgagt ggatttaagg cgggaaacgg cggcattgaa
     3001 aagaaaagac cagctatact ccaccttttt cgaaaaatat agtattatac gatacggaga
     3061 agaagaaccg caaaacccga ctgcaagtgc tccggcgctt cattcggact catgttttca
     3121 tccgggaggc tgccttcttg agagacggaa aacttttgct tcgcatcaca cttgtactcg
     3181 tcctctgcct ccttgcacgg cagagttccg cttacgtaaa gatactgacg gcccccggac
     3241 accccgtatc tttgattgtc gaacatgacg aggggcgtat ccggaaagcg ttcttccgtt
     3301 cgccggaggg agtacgtccc ttggaacaca tcgaacagca tcttcttctt tcggattcgc
     3361 ttacttttct gtgcgcagac gacgacatcc ttgacgactt ggtgtggaaa atcgacctcc
     3421 tgcaaccgaa gaccgaaaag atgctctctc tctggataac atccgtcacc gaagcatcta
     3481 ccgcctggct tgcactggcg ccgtcaggcg ctggcttttg ggaatcgctt cctcgggcgc
     3541 cgctccccag ggaagacgtt tttctctaca tcgcgccgca tctccctgcg taccaagagc
     3601 tcggccagcg aagcggaccg gaaatcctga cgttcattta tacgatgctc ctcacgaaaa
     3661 acggcccgaa actggcggcc gtcccggaaa tatacagaca gtttcttccc ctgaccgctc
     3721 ttgtctgccg ggcacaggaa aacaaggacc tcaaagcggc ctacacggcc ttgcatcagg
     3781 actttgagcg gatggggcaa ggaggaatgc cttcacggga ggccatagat aactttctgt
     3841 ggaaacgcat tcttactgtt cgatggaaac gatgacggaa gaagtgcggg gcgccccgtt
     3901 tcggcggcgc cccgtttttt accctgcacg ttctttcagg gcatgaagaa cgtcttctgc
     3961 gtgaccggct actttcacct ttgagaagag acgggcaacg atcccgtcag gggtgacaag
     4021 caacgtagaa cgttcgactc ccatatactc cttcccgtac atcttcttct gcttccagaa
     4081 accgcatgct tcaatgaact catgctcagg atcgctcaaa aggggaaagg atatgtcaag
     4141 ttttgcagca aacttgcggt gactttcgac cgaatcaggg ctcacaccaa caattcggca
     4201 gttcagctct tcaaaagcgg acatcctgtc cgcaaagtcc ttgacttcgt ttgttcaccc
     4261 cgacgtattg tccttgctgt aaaaaaagag cgccagccag ctccctgaaa aatctttatg
     4321 cgaaaactcc ttccctgatc catcctgaag gagaaaacct ggcacgggct tcccttccat
     4381 tctttttccc tccttaacgg cacattccgt cgagtaactt cttcacggcc tctcgcggcg
     4441 cagcgcggcg gaagagcgga tttttgtcgt tcctgtatgc ggccagcgac ccctcgaagg
     4501 tcgtccgttc ttctccgcga aaaagaactc ccagcggaaa aggctcctcg gacagggcaa
     4561 gcgagagagc cttcgagaaa tccgaaggat cgtgcccgtc cagccagtat gtgttttcct
     4621 tgaaccacgc gtaggtgttc actgaattga aggagacgca cggctggaag atatcgacaa
     4681 gggcataccc cttgtactga acggcttcct tgatgacgtc cttcgtcttt tcgacgtcgc
     4741 ccgcataggc ccttgcgacg aaaccggctc cctgaacaag cgccgtggcc aaggggttga
     4801 aaggctgcgc atggacgccg tccacctgca cggacgtacg aaaacccctc ggactggtcg
     4861 gggaagcctg ccccttggtc agaccgtaca ccatgttgtt gaataccagg ttcgtgatcc
     4921 ccggatttct tcgtatcgta tgtaaaaagt gattgccgcc ctctccgtac atatcgccgt
     4981 caccccctac ggcgacaact tcaagcgacg gatttgccgc cttaatcgcc gtagcgttgg
     5041 gaagcgctct gccatgcagt ccgttgaaga agtgcgactt catatagtga ggcgtttttg
     5101 ccgcctgtcc gattccggag acaacgacaa catccagcgg agaaagccct aactcttcga
     5161 gcgcctgttt gatagaatcg agaatcttga aatcaccgca ccccgggcac caggagattt
     5221 cgatgccggg aaggtcgaag gacgtaccca tcacgatctc cctcctgttc cggaaaaaac
     5281 ttccgacagt ctgcgcgcag cctcttcaac cgagaactga aggccgttgt atttcaatac
     5341 cgtgtccgtc gcggcaacgc cagtctgcgc gcggagcaag cgcgcgaact gcgcagtgct
     5401 gtttccctcc gcggcgacga tgcgacgagc tcgtttcaac agcgcatcta ccgaggaatg
     5461 aagcggccaa acctgttcga aagccagaag agctgtttgc ttcaaatcca ggagcgtcaa
     5521 cgcctccttg aaaatcggaa tcgtcgaccc ccaacagagt acaaggtccg taaattcctc
     5581 ggccccgtac agcttcggcg caagcgcttc ttcacgcatg ccctcaagct ttctcatccg
     5641 tttttcctgc attcgtttcc gaagcttgaa attctcgtag acgtgtccga cctcgtcgtg
     5701 ttcatgactg tccgcgccga cgataccgct cccccacccc ggaacgccga acggggaaca
     5761 gccggatgcg gtctcttcat agcgtctgta cccctccccg gtctcccgga caggagacgg
     5821 cggttccgag aaaaacaagt cgtcggggcg cacattatag aaagaattca caaaatactg
     5881 atcggtaagc acaatggcgg gcgtctggta cttcagcgcg accctgaacg cctcttgtgt
     5941 cagcgaaaaa gcgctttcga tgctccccgg cgcgtagatc gcccgtggaa attcgccgtg
     6001 ccccgagtac agcgcaagtt cgagatccgc ttgttctgtt ctcgtcgcca tcccggtagc
     6061 aggacctggc cgctgcgaaa gatggactac aacaggagtc tccatgacac ctgccaaact
     6121 cagtccctcg cccataaggg caaagccgcc tcctgacgtg gtaaccaagg gacgcgcgcc
     6181 ggtataggag gcgcctacag ccatgttgat cgcagccagt tcgtcctcta cctgctctac
     6241 ggcagcgcca gcctactccg catgcttcgc gaagaaggag agcacgccgg tggccggaga
     6301 catcgggtag gctgtcacga aattacaccc ggcggccata gcccccaaag agactgcgtc
     6361 gcttccgttg atcagagcct tcccgacttg aagatccgcc tgcattcccg agagaagccc
     6421 cttccccttt accagcttct cgccgagctc gtatcccgac cggcaggccg tcgtattttt
     6481 ccccgcgaca tcgcctttct tcccggagaa acgctcctcg aagaatgctt ccgccttctc
     6541 cgttcccagc ccaagaattc ccagaagaat acccgccgct acagaattcg agtaaatacg
     6601 acctcctgcc tcggctgcaa gccgggaaag cgggacatca acaaacagcg ctccggcgtc
     6661 ctcaagctct cccttcagtt cctccttatc gccgatgatc agcgtggaag gcgtgatacg
     6721 ctctcgaata ttctcccgca cgttccggct cagggccacc aggatatcca tcctgtccac
     6781 aagcgcatcg acacgaaacg gcgctatacg cagctccgtc gagttgttcc ctcctcttac
     6841 cctggacatg tactcgcgtg atccgaaaac ggaaaaaccg gaatcaacga caatacgggc
     6901 gagcatgtct tcaactgttt gaattcccag accagcagcg ccggagagaa cacaggatac
     6961 ttccttcacc tctcccgaaa atggaaagat catagtcgtc ctcccttcaa aaaagtacgt
     7021 taccctgttt taaattatac agctttctac ttcccgaagc gctcgttcag caaatccact
     7081 tcttttcgac cacatgctat aatatctcat gaagtatgga cgatggatat agggtaactt
     7141 tgattttcca agaaaatacg gaggtgtttt tatggggaaa tttagatgtc tatcgtgcaa
     7201 aagcgaattc gagttggacg aaaaaaactc gaccatgcga tgccagaggt gtggcagcag
     7261 attccttgag gtaattgacg ccgaaaacag gaaacggggc aaatcctgga gcgcaaaatc
     7321 cttcagcgta gggaaaagca caaaataatc tgtctaaaag gccaaataaa ggaaggcgcc
     7381 gaaaaaaccg gcgccttcct ttatttttgc agcgttcgtt tctgtacaac ccttgaacta
     7441 gagctcagct acgccttgat atctggattt ttaagcgcct tcagagattc gaagaccatc
     7501 atgatcgcca gaaccaaaag gataatggag ggaacaacca agatgtaatt aggatgctca
     7561 tacgccatgt tgtccttgat caagaaacca agagccgcaa cagttgtgac aagcatgaac
     7621 cacatcgggt acatcgcgaa tcgattgtcc ttcttcagag ccttaccaac ccaaacgccg
     7681 atagtcaaca gcgcaagcgc cgcaacaagc tggttggaag cgccgaagat cggccagatc
     7741 gccgcccacg cgggaattac gtttcccgtg gggccgtgag ccttcgtaag aagcagagcc
     7801 ataccggcgg caactgcgat cacggtggcg gtatatttgt cgatcttcat gttcgaaagc
     7861 tcctgaatct ggtaccgggt caaacgggtt gccgtatcaa gcgacgtcag aaggaaggag
     7921 ttaatagcca aaaggccgag agacataccg accttcgggt cgatgccgat tatcgcggcg
     7981 aactttccga atccctgcgc ataggtgatc gtcgggccgc cctgcgcgat ggtgccggta
     8041 atcatg
//
LOCUS       DAOPKU010000062         8041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9516_length_8041_cov_4.994866, whole genome shotgun sequence.
ACCESSION   DAOPKU010000062 DAOPKU010000000
VERSION     DAOPKU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9516_length_8041_cov_4.994866"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PK849_03095"
     CDS             <1..760
                     /locus_tag="PK849_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HPE65141.1"
                     /translation="RMKERGLGVYAFTGPFFVTGAEPGDVLEVRIKRILPGQYAVTHI
                     YPDETGIGGLPEGFETGYLKPLYLSEDRKSIPFAPGVTVPVRPFLGTMAVAPRPGEEH
                     PPAVPGYFAGNMDNKELGAGSTLYIPVNVPGALFMAADAHAVQGDGEVSIAAAETYFE
                     EVELEFVVRKDLPLRFPMAETPDHWIVMAFHKDLDEAMKGAIREAIRFLEREKRLTRN
                     EAYSLCSLAVDFRVTQVVDGDKGVHGMIPKAIFG"
     gene            complement(860..2161)
                     /locus_tag="PK849_03100"
     CDS             complement(860..2161)
                     /locus_tag="PK849_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPE65142.1"
                     /translation="MASLLENRFHLKEAGTDVKTEVLAGITTFMTMAYIIFVNPGILQ
                     NAGMPFGALMTATCLASALATFLMAFMANYPIALAPGMGLNAFFAFSVVLGMGISWQV
                     ALAAIFVEGILFILLTLTKIRESVVNGIPKSLKIGISTGIGLFIAFIGLQSAGVIVKN
                     DAVLVGLGNLKSVEVLLACLGFFIMGALEARKVKGSILWGILIITGIAVFLGVAKAPE
                     ALVSTPPSVMPIFMKMDFSGLMNPNFWIVMFSFFFVDFFDTVGTLVGVTNRAGMLDAE
                     GRLPRAREALMADAIGTTAGAVLGTSTVTSYVESASGVEQGGRTGLTALVTGVLFLLA
                     LFFSPIVSIVPACATAPALVLVGVFMMASLKDLDFSDWTDFVPASVAIFVMPFSYSIA
                     HGIEFGIISFVVLKVLGGKAKDVSPIMLVLAALFVAKEIFF"
     gene            complement(2195..3922)
                     /gene="ade"
                     /locus_tag="PK849_03105"
     CDS             complement(2195..3922)
                     /gene="ade"
                     /locus_tag="PK849_03105"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPE65143.1"
                     /translation="MNVDDFLAVARGDREADLVLRGARVANVFTLEYEEADVAIYGGR
                     IAGVGKGYSGRAEEDLSGCVLAPGFLDGHCHIESTMLTPAGFAELAAVRGTSAVAADP
                     HEIANTCGMAGVEFMWRESLGCPVDIHYTAPSCVPASSFETPLEPLDAGAIAEMFERG
                     WCDSLGEVMNYPGVINGDPALWAKLYASGKRPRSGHAPGLSGKELCAYLLSGCDSDHE
                     SFSIEEGLEKLRRGMWLMIREGATEHNLEELAPIVKENEARSSRCMMVSDDLTARYLR
                     DVGHMDEKIRRAVRLGISPLVALRMVTLSTAEYFGWRDLGAVAPGRFADLVAVDSLEN
                     CSVRKVWKRGRLVAENGKLAGPRVSRVAPPVLAAGALSVPEESFFHLPVPAGAKIRVV
                     GLRRGQVVTEHLVMEPTVRNGMASADPERDLAYLAVLEKNRGSGRLAVGFVHGLGLKE
                     GAICSSVAHDAHNFVAAGMDIPSLRRAFALLIEQGGGIAAVRGDTVLASLALPVGGLM
                     NPSPADAVISGFEEVERAAATLGTDVNHPFMAMSFLSLSVIPELKLTDQGYVDLGRGG
                     EQSLFVEEA"
     gene            complement(4086..5252)
                     /locus_tag="PK849_03110"
     CDS             complement(4086..5252)
                     /locus_tag="PK849_03110"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HPE65144.1"
                     /translation="MKKYRILASFLILALCIVAAPASAKYMIVGNDEKMTWNEKGTVV
                     NLPSGKDTVQIFDISEGGTEPKLLATLELMNSISGPPTNLAITKDEKLAVLANPLEWA
                     EKDGKWSGSPSTKIYVIDLEGDAPKHIATVDGGRQPSGLAINRAGDLMLVANRADNSV
                     GVFSIEGKTVTLIDTVPMDEQVAGVAITPDGTKAWVVKNLSHKLALLNIDGKKVTPAK
                     FEIPVLWPYNVDITPDGSLALGADMGGPGGSSGHVDTVSVIDMSQNPPRVIDRVVAGD
                     GPEGFAVSPTGEYLATVNIRGSNVAHDSWFYHKNGTVSLLKIDGKKLTKVNEVEVGAL
                     PEGVVWSPDGKWLYVGNFLDKNISVLKIEDGKLVDSGVKIPVAGSPASMRGPVN"
     gene            complement(5632..7284)
                     /locus_tag="PK849_03115"
     CDS             complement(5632..7284)
                     /locus_tag="PK849_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HPE65145.1"
                     /translation="MSEQLEHTSRQEEPRYDPHYYPYPSRRNMTYGSRGMVCASHPLA
                     AQAGLDALKKGGNAVDAAVATAAALTVVEPTANGIGSDAFAIIWKDGKLSGFNASGPI
                     PKALSMELAESKGWTASGKLPTYGWTPSTVPGAPAAWAELTKRMGRLSLEENLAPAVF
                     LARDGHAVSVTTAHHWELAFKKYALEKGEEFRAWFDTFAPSGHAPRAGELWRSEAHAR
                     TLERIAGSNARDFYEGEIAAKILEAARKTGGYFSAEDLGSFVPEWVDPIGVNYKGYDV
                     WEIPPNGQGIVALIALSILRGFEFSSHDDPMTVHRQIEAIKMGFADAHRYVADTRFSG
                     VPTAELLAAPYADARRALLGERAEARNAGNPLPGGTVYLCAADGEGNMISYIQSNYMG
                     FGSGVVVPGAGVALNNRGHCFSLEKGHPNMLEPGKRPYNTIIPSFLTKDGVPVGPFGV
                     MGGFMQPQGHVQVVMNTVDFHMNPQQALDAPRWQWMGGMNVSVEPGFSPALAQSLQRK
                     GHAMKSELESNSFGRGEIIWRTPEGTLGGATESRTDGCAATW"
     gene            7601..>8041
                     /locus_tag="PK849_03120"
     CDS             7601..>8041
                     /locus_tag="PK849_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPE65146.1"
                     /translation="MRIPYDEMQDVFLRILMKYGFSEEKAARAAELFTDNTCDGVASH
                     GANRFPRVLSYIRKRHIDPAADPVIEESFGALERWNGNRGMGCTNASSAMDRAMELAG
                     LSGVGCVAMRNTNHWMRGGAYGLQAARKGYTGICWTNTMPNMPPW"
BASE COUNT         1593 a         2498 c         2479 g         1471 t
ORIGIN      
        1 gcgcatgaaa gagcgcggcc tgggcgtgta cgccttcacc gggccgttct tcgttacggg
       61 agccgagccg ggagacgtcc tcgaagtccg tatcaagcgc atccttcccg gccagtacgc
      121 cgtcacgcac atctaccctg acgaaacggg gatcggcggc cttccagagg ggttcgagac
      181 cgggtatctc aaacctctgt acctctcgga ggaccggaaa agcatcccct tcgctcccgg
      241 cgtgacggtt cccgtccgtc ccttcctcgg gacgatggcg gtggccccgc gccccggcga
      301 ggagcaccct cccgcagtcc cgggctactt cgcggggaac atggacaaca aggagctggg
      361 ggcgggaagt acgctctata tcccggtgaa cgtgccgggg gcgctcttca tggccgccga
      421 cgcgcacgcc gtgcaggggg acggcgaggt gagcatcgcc gcggcggaga cctacttcga
      481 ggaggtcgag ctggaattcg tcgtccgcaa ggacctgccg ctgcgctttc cgatggcgga
      541 gacgccggac cactggatcg tgatggcctt ccacaaagac ctcgacgagg cgatgaaggg
      601 agccattcgc gaggcgatcc gcttcctcga acgggagaag cggctcaccc ggaatgaagc
      661 ctactccctg tgcagtttgg cggtcgactt ccgggttacc caggtagtag acggagataa
      721 gggagtgcac ggcatgatcc cgaaggcaat tttcgggtaa gcgacaccgg cacggacagc
      781 ccatgaaaaa ggggagcgcc ctgcggttcg gagcgctccc cttcgcgtag tgtactgtgc
      841 ctgatcggcc ggcgcggcgc tagaagaata tctccttggc gacaaagagc gctgccaaaa
      901 caagcatgat cgggctgaca tccttcgcct tgcctccgag taccttgagg accacgaagg
      961 agatgatgcc gaactcgata ccgtgagcga tgctgtagct gaagggcatg acgaagatcg
     1021 ccacgctcgc cggcacgaag tcggtccagt cgctgaagtc caggtccttc aagctcgcca
     1081 tcatgaagac gccgacgaga acgagggcgg gagctgtggc gcacgccgga acgatggaga
     1141 cgatgggaga gaagaagagg gccagcagga aaaggacgcc ggtcacaaga gcggtcagtc
     1201 cggtgcgtcc gccctgctcg acgccgctgg cgctctcgac atacgacgtg acggtggacg
     1261 ttccaaggac ggcgcccgcc gtagtgccga tggcgtcggc catgagggcc tcgcgggccc
     1321 ggggaagccg tccctcggcg tcaagcattc cggcgcggtt ggtcacgccg acaagcgtcc
     1381 cgaccgtatc gaagaagtcg acgaagaaga aggagaacat gacgatccag aagttcggat
     1441 tcatcagtcc ggagaagtcc atcttcatga agatcggcat gacggaggga ggcgtggaca
     1501 ccagcgcttc gggcgccttg gccacgccca ggaagaccgc aatccccgtg atgataagga
     1561 taccccaaag gatggagccc ttcacctttc gcgcttcaag cgctcccata ataaagaagc
     1621 cgaggcaggc gagcagcact tcgacggact tcaaattgcc cagcccgacc agaacggcgt
     1681 cgttcttcac gatgacccca gcgctctgca ggccgatgaa tgcgataaaa aggccgatac
     1741 ctgtcgagat gcctatcttg agcgacttgg gaataccgtt gacgacgctc tctcggatct
     1801 tcgtgagcgt gagcaggatg aagagaatgc cttcgacgaa gatcgcggca agcgcgacct
     1861 gccaggagat acccatgcca aggacgacgc tgaaggcgaa gaaggcgttg agccccatcc
     1921 cgggcgccag cgcgatcggg tagttcgcca tgaacgccat caggaacgtc gccagagcgc
     1981 tcgccaggca ggtcgccgtc atcagcgctc cgaagggcat tcccgcgttc tggaggatgc
     2041 cggggttcac gaagatgatg tacgccatcg tcatgaacgt ggtgataccc gccagaactt
     2101 ccgtcttcac atccgtcccc gcctccttga ggtgaaagcg gttttccaac aatgatgcca
     2161 ttataattcc tcccattcta tagtgagaat atgactacgc ttcttcaaca aagagtgatt
     2221 gttccccgcc cctgccgagg tcgacatacc cctggtcggt cagcttcagc tccggaatta
     2281 ccgagaggga gaggaagctc atcgccatga aggggtggtt gacgtcggtt cccagggtcg
     2341 cggcggcccg ttccacctcc tcgaatcctg agatcaccgc gtcggccgga gagggattca
     2401 tcaaacctcc gacggggagt gcaagagagg caaggaccgt atcgccccgc accgcggcga
     2461 tgccgcctcc ctgttcgata aggagggcga aggccctccg cagcgaaggg atgtccatgc
     2521 ccgccgccac aaagttgtgc gcgtcgtgcg ccacggagga gcagatggct ccctccttca
     2581 gcccgagtcc gtgaacaaat ccgacagcga gcctgccgct cccccggttc ttttcgagga
     2641 cggcgaggta ggccagatca cgctcgggat ccgccgatgc cataccgttc cgcacggtcg
     2701 gctccatgac gagatgttca gtaaccacct gcccccggcg aagtccgacg acgcgaattt
     2761 tggcgcctgc ggggacggga agatggaaaa aagattcctc cggaacggaa agagcccctg
     2821 cggcaagcac aggcggggcg acgcgggaaa ctctgggacc ggcaagcttt ccgttctcgg
     2881 cgacgaggcg tccgcgcttc cagaccttcc ggacggagca gttctccaac gaatccacgg
     2941 cgacgaggtc ggcgaacctg ccgggagcga cggcccccag gtcgcgccag ccgaagtact
     3001 cggccgtgga gagggtgacc atcctgagcg cgacgagcgg agagattccc agacggaccg
     3061 cccggcgtat cttctcgtcc atgtgaccga cgtcccggag gtagcgggcg gtaaggtcgt
     3121 cgctcaccat catacagcgg ctggagcgcg cttcgttctc tttgacgatc ggcgcaagct
     3181 cctccaggtt gtgctccgtg gcgccctcgc ggatcatcag ccacatcccg cgacggagct
     3241 tttcgagccc ctcctctatt gaaaagctct catggtccga atcgcacccc gagaggaggt
     3301 acgcgcacag ctccttaccc gagaggccgg gggcgtgccc ggagcggggt cgcttcccgg
     3361 aggcgtacag cttggcccaa agggccgggt cgccgttgat gacgccggga tagttcatca
     3421 cttctcccag cgagtcgcac cagccgcgct cgaacatctc ggcgatagcg cccgcgtcca
     3481 ggggttccag cggcgtctcg aaggacgagg cgggaacgca tgaaggagcc gtataatgga
     3541 tgtccaccgg gcagccgagg ctctcgcgcc acatgaactc gacgcccgcc ataccgcagg
     3601 tgttcgctat ttcgtgcgga tcggcggcga cggcggacgt gccgcgaacc gcggccagct
     3661 cggcgaagcc cgcaggagtg agcatggtgc tctctatgtg acagtgtccg tccaggaagc
     3721 cgggagcgag cacacaaccg gagaggtcct cctcggcgcg tccggaatac cccttcccca
     3781 ccccggcgat ccgtccgccg taaatggcga cgtccgcttc ctcgtattcg agggtgaata
     3841 cgttggcgac cctggctccg cggagcacca gatcggcctc gcggtcgccc cgagcgacgg
     3901 cgagaaaatc atcgacattc acatgcaatt cctcccttct ttttccatga tgaagtgtaa
     3961 cggaaagatc gccgatgcgc aatcggaagc gatgaaaagt tgcagaatcc gggaatgcat
     4021 ccgtatacgg gtacaaaaaa gcccccgcct ggagaaacag caggggcttg tatgcttcgg
     4081 ttgcttcagt tcacagggcc gcgcatggat gccggagatc ccgcaacggg tatcttcaca
     4141 ccgctgtcaa cgagctttcc gtcctcgatc ttgagcacgg agatgttctt gtcgaggaag
     4201 ttgccgacat acaaccactt gccgtcgggg ctccagacga cgccctctgg gagcgcgccg
     4261 acctcgacct cgttcacctt cgtgagcttc tttccgtcga tcttgagcag agagacggta
     4321 ccgttcttgt gatagaacca ggagtcgtga gcaacattgc tcccccggat gttcacggtc
     4381 gcaaggtact ctcccgtggg agagacggcg aacccttcgg ggccgtcgcc cgcgaccaca
     4441 cggtcgatca cgcggggcgg attctggctc atgtcgatga cggagacggt gtccacatgg
     4501 ccggaagagc cgccggggcc gcccatgtcg gcgcccaggg ccagggagcc gtccggcgtg
     4561 atgtccacgt tgtacggcca gagcacgggg atctcgaact tcgccggggt tactttcttt
     4621 ccgtcgatgt tgagcagcgc gagcttatgc gaaaggttct tgacgaccca ggccttcgtg
     4681 ccgtccggag tgatggcgac cccggcgacc tgctcgtcca tcgggacggt gtcgatgagg
     4741 gtcacggtct tcccctcgat ggagaagacg ccgacggagt tatccgcccg gttggcgacg
     4801 agcatcaggt cgccggccct gttgatcgcg aggcccgacg gctgccgtcc gccgtcaacc
     4861 gtcgcgatgt gcttcggcgc gtcgccttca aggtcgatga cgtagatctt cgtggagggg
     4921 gagccgctcc actttccgtc cttttccgcc cattcaagag ggttcgccag gacggcgagc
     4981 ttctcgtcct tcgtaatcgc caggttcgtg ggaggtccgg agatggagtt catcagttca
     5041 agagtggcca gaagcttcgg ctcggttccc ccttcggaga tgtcgaagat ctgcactgta
     5101 tcctttccgg aaggcagatt cacgacggtt cctttttcgt tccacgtcat cttctcgtcg
     5161 ttgcccacga tcatgtactt cgccgaagcc ggcgctgcga cgatgcacag cgcgaggatc
     5221 aagaaagacg caagaatccg gtactttttc attgaggcac gctccttcta taccaaacca
     5281 acactacgcc gatacacgcg atgtcccgac cttaccgatt tgcgcatgca ctcgctcatt
     5341 accggttccc tatcacctcc gggggctgcg aaacgctttc ggtcatcatt agagaacccc
     5401 aaacgtttcc cgccgaaaaa ataacctgat tctacaacgc gatattttac tgcaaaaaag
     5461 tttattttac tccccttaga cttcccaaaa acaaaaaata catgttcgga cctttacgtt
     5521 cccggacgtg tttctctttt cgagacaaac gtattcggcc gaacaactcg taaaaataaa
     5581 gcccccccga tgctccggag aggcttcgcg cgtcggctga aacgagagag attaccaggt
     5641 ggccgcgcag ccgtcggtgc gcgattccgt cgcgccgccg agcgttcctt cgggtgttct
     5701 ccagatgatc tcgcccctgc cgaaggagtt ggactccagc tcggacttca tcgcgtgccc
     5761 tttgcgctgg aggctctgcg caagggcagg agagaagccg ggttcgacgg agacgttcat
     5821 cccgcccatc cactgccagc ggggggcgtc gagcgcctgc tgcggattca tgtggaagtc
     5881 gacggtgttc atgacgacct gcacgtggcc ctgcggctgc atgaagccgc ccatcacgcc
     5941 gaacggcccc acggggacgc cgtccttcgt gaggaaggag ggaatgatgg tgttgtacgg
     6001 ccgcttgccc ggttcgagca tgttcgggtg ccctttttcg agcgagaagc agtgtccgcg
     6061 gttgttcagc gcaacgccgg cgccggggac cacgacgccg gagccgaagc ccatgtagtt
     6121 gctctggatg taggagatca tgttgccctc gccgtcggcg gcgcagaggt agaccgtccc
     6181 gccgggaagc gggttcccgg cgttccgcgc ttctgcgcgt tcgcccagaa gagcgcggcg
     6241 cgcatcggcg tacggagccg ccagaagctc cgcggtcgga acgccgctga atcgggtgtc
     6301 cgcgacgtag cggtgcgcgt cggcaaagcc catcttgatc gcttcgatct gccggtgaac
     6361 ggtcataggg tcgtcgtgcg aggagaactc aaacccgcgc agtatcgaga gcgcgataag
     6421 ggcgacgatt ccctgtccgt tggggggaat ctcccagacg tcgtaccctt tgtagttcac
     6481 gccgatggga tcgacccatt caggaacaaa agacccaagg tcctcggcgc tgaagtaacc
     6541 accggttttc cgtgcggctt cgaggatctt cgcggcgatc tccccctcgt agaagtcgcg
     6601 cgcattgctc cccgcgatcc gctccagcgt gcgggcgtgc gcctccgatc tccagagctc
     6661 tccggcgcgg ggtgcgtgcc ccgagggggc gaaagtatcg aaccaggcgc ggaactcctc
     6721 ccccttctcc agcgcgtatt tcttgaacgc cagctcccag tggtgcgccg tcgtcaccga
     6781 gaccgcgtgc ccgtcgcgcg cgagaaagac cgccggggcg agattttcct caagagagag
     6841 ccgccccatc ctcttcgtca gctccgccca cgccgcagga gcgccgggaa ccgtcgaggg
     6901 agtccatccg taggtcggca gcttgcctga agcggtccac cccttcgact cggcaagttc
     6961 catggagagc gccttcggga tgggaccgct cgcgttgaat ccggacaatt ttccgtcctt
     7021 ccaaataatg gcgaaggcat cgcttccgat gccgttcgcg gtcggctcca ctacggtcag
     7081 cgccgcggcg gtggccactg cggcgtccac cgcgttgccg ccctttttca gcgcatccag
     7141 cccggcctgg gcggccagcg gatgcgacgc gcacaccatg cctcgcgaac cgtaggtcat
     7201 attcctgcgg gatgggtagg ggtagtagtg cggatcgtag cgcggctcct cctgccggga
     7261 agtgtgttcg agctgttcac tcatcgttct ttcctcctag aaaaagagtt ttcttcgtcg
     7321 atttcctcga atcagcctgc gcctgccagg cgctcgatat atctattgca ggataccaca
     7381 tatttcctga aagtcgctca gtaacggaga agagcccgca gaaagacgcc gaaccactca
     7441 cgacggccgc attgtttcat ccagagaaac gcaagtttat cctgtcgaac gttgacgaaa
     7501 ttctcctcca tgggtaagat taaagagaag cggcaagagc ttcccgggaa gagacaaggt
     7561 cccggttttt taaccgaaac acgggagggg atttcgagag atgcgcatac cgtacgacga
     7621 gatgcaggac gtgttcctac ggatactgat gaaatacgga ttctccgagg agaaggcggc
     7681 gcgcgccgcc gagctgttca ccgacaacac ctgcgacggc gtggcctccc acggagcgaa
     7741 ccgcttcccg agggtgcttt cctatatccg caagaggcat atcgaccccg ccgccgaccc
     7801 ggtcatcgag gagtccttcg gcgcccttga acgatggaac ggcaacagag gcatggggtg
     7861 cacgaatgcg tcctccgcga tggaccgcgc tatggaactc gccggtctct ccggcgtggg
     7921 gtgcgtcgcc atgcggaaca cgaatcactg gatgcgcggc ggggcctacg gccttcaggc
     7981 cgcgcgtaaa ggctatacag gcatctgctg gacgaacacc atgccgaaca tgcctccatg
     8041 g
//
LOCUS       DAOPKU010000063         8039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9521_length_8039_cov_4.412450, whole genome shotgun sequence.
ACCESSION   DAOPKU010000063 DAOPKU010000000
VERSION     DAOPKU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9521_length_8039_cov_4.412450"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /gene="rsmH"
                     /locus_tag="PK849_03125"
     CDS             <1..867
                     /gene="rsmH"
                     /locus_tag="PK849_03125"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPE65147.1"
                     /translation="LSHYFVCVLAYVLDCTLGLGGYSEAILESFESTEVYAVDQDSQA
                     LSFAKERLLPFGARFHPEKGNFASLPPAVTCNAPFDAMLFDLGVSNLQLVDEDRGFSF
                     QSDGPLDMRMDREFRYSAADLVNSYSEREIADIFWKYGEERFSRQLARGIVRYREESG
                     KIERTSTLVDIVRKTLPAPVQRKMGGHPARRIFQALRIAVNDEISILETGLHNIPDVA
                     APGALLVVVSYHSLEDRIVKNLFKKWREEGKGSIVTKRPLVPSDEEIEANHKSRSAKM
                     RVFSFKPKRMEV"
     gene            875..1294
                     /locus_tag="PK849_03130"
     CDS             875..1294
                     /locus_tag="PK849_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65148.1"
                     /translation="MRFREDIKKETVSDSFHKISPYIIVFTLCAFGLFMGLGVLRLYS
                     FRLECKLNSVNRQIESYKANRIVLKHELSSLVSPARIYGFSKQEMGMTFASNVRILRV
                     EEHLLAEASFESSPVVASKQENEGWFFFFLEGAMAGE"
     gene            1326..3002
                     /locus_tag="PK849_03135"
     CDS             1326..3002
                     /locus_tag="PK849_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPE65149.1"
                     /translation="MTRRFGSPWFLVFIFFGVVIWRLTSLQLFPDPRVENQSRRQYWI
                     HVPVSTNRGFIYDRNGNALAVSVPSFSFFLDPAFWNPSDAVHLKGILPDSLVDTLSKP
                     LSGRFLWLARKVDIETAEKIRSLKIKGIFEIEEKKRLYPSGSLLSHVLGFCDIDDKGL
                     SGIELMWDKALFSPPGLRVLAKDSSGRTLDISRNDHDHTPTEGGNVSLTIDSRIQFIV
                     ERRLEEGIREHSARWGSIICMDPQTGAILSMASWPTFNPNVRTDLSKTQNLLNNSIGR
                     TYEPGSTFKPVVLGISMERGSVHPNESFHCPYRIKIADGHISEAGKNGFGRLSVPEIL
                     AKSSNTGMAQIGIRVKPFNMYHSVREWGFGKPTGIELPGAEDGLLSSPEQWRGVVPSN
                     IAIGQGLAITPLHLASAISAIANGGNLLRPHIVSKVVDSSGNIVYNEERQFLRTVLSP
                     QVALWLQRAMVDTVVKGTGRNGAVPGISIAAKTGTAQIAERGEYAKGRWVSSFAGFWP
                     SSDARYVVLVVIGEPSKGKYYGGDVAAPVFRRIVEDMEQIGLLAAVKTEG"
     gene            3010..4524
                     /locus_tag="PK849_03140"
     CDS             3010..4524
                     /locus_tag="PK849_03140"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPE65150.1"
                     /translation="MILSKIIEEYAARYSSPLRLHNQAIDGGTDVSVVHVGCNSLEIK
                     KDELFCCIRGEHNDGHSFAGDAVQKGASTLLVERPLPLETPQIVSSNVRRDMGRLSSI
                     VYGDPANTLKMFAVTGTNGKSTTTWIIRHLLQSIGIRTGLLGTIVYNDGIEEKDAART
                     TPESCDIQLMLLRMLQNGCGACVMETSSHGLSFGRIEGCSFDAAVFTNLSEEHLDYHG
                     TLENYFSAKSRLFSDYMKQNWAGGANGDDAFARRLLGLYSDKLVSFGIEKEYGAHLLG
                     SNLQSSLEGTRFSLSLPGVSEPVPVFLPLPGKFNVYNALAALSATLPYATDKLALVDS
                     LHSMIQVPGRMERYSFNSGVSCIIDFAHTPFALQNVLTEVRRLCGGRIISVFGHGGER
                     FQPNRFSLGNIAATLADEVIVTMDNPRNEPAEQIAAQIVEGVESSERRIPLKTILDRS
                     AAVYEAMDSAKPGDVVVVSGKGPEKYLRIGSESIPYSDGDTLRRWADERGVEWR"
     gene            4521..5903
                     /gene="murF"
                     /locus_tag="PK849_03145"
     CDS             4521..5903
                     /gene="murF"
                     /locus_tag="PK849_03145"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPE65151.1"
                     /translation="MKNDLRLSCIAALADTHLTGPDGPIPLRFVADSRLVVPGDAFVA
                     FRGEQVNGHDFIHDAVSRGASLIICEESAGVPNHVASIVVDNCFSAIPRMARRYLEIH
                     RESLEVLAVTGSVGKTTTREMIRTCLAPSFKVHSAEHSYNTLIGCALSILAMPADTEI
                     LILEMGTNHPGEIGEMVDFFPPTISMITEVAPAHLEGLGSVEGVAQAKFEITGSPFLK
                     AFFYFGDNPLLVSGAASLPSHVRSLSVGFGENDFSIRDTEFILSNGLPRLTFDVVYKG
                     KTFHASSPLFGRHSALAAGFGIALSLYLGASIEQALQSIESLQPPCGRGRFFLLTSGA
                     MLLDDSYNANPASMKASLSESLRIPVQRRIAVLGEMKELGKSALDYHRELLLFFTKFS
                     KVFIMGTLWRTLFQNTPLPDNTVFFDNPLSLGTEIKKELGEGDLIVVKGSHGNRLDFL
                     VDFLVEEGVE"
     gene            5900..6862
                     /gene="mraY"
                     /locus_tag="PK849_03150"
     CDS             5900..6862
                     /gene="mraY"
                     /locus_tag="PK849_03150"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPE65152.1"
                     /translation="MIYALSWAFLFFLQIFCSKIWIARLHQWRVVQIQKSYGLQSHIE
                     TKSSTPSMGGVVFLLIGIASLLPEAVFSETRLSNAALFWILPLGSAAIGFIDDWIKLT
                     RKSSEGFPSLYKLAAQILLTVPWSCWIVFSQGLSLWPGVVVPALLAVPLLTIVSIGML
                     NAVNVTDGLDGLAAGAALFSFIGAIVLLPLESFLLYPALAGSALCAGFLWHNAHPAKV
                     FMGDIGSHFLAGMLVALCAHSEYFIAVIPLGFLFGVEIVSVAMQLIAIHCFKRKVFRM
                     SPLHHHFELLGWSETQIVTRFWIIHGAGLAFLALFVQGILWKAV"
     gene            6979..>8039
                     /gene="murD"
                     /locus_tag="PK849_03155"
     CDS             6979..>8039
                     /gene="murD"
                     /locus_tag="PK849_03155"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPE65153.1"
                     /translation="MAHRLGAQVFVSDEKKLQDQSLVALFQSHGIEWEDGGHTPRLFE
                     ADYILVGSGISPNAKPVTESNRRNIPVIGELDFISPFLRSRIIGVTGSNGKSTTTALA
                     GYLLQKAGFSAEVAGNIGKSLADFAFVQRDFLVVELSSFQLHWNTRFACDLAMVTNLA
                     PDHIDWHGSYEEYIRAKAKILSTQSPGGFAIVQETERSVLFGDREDDAHEVLAFSWNG
                     MQSSHGRKLLADEKACHVLFRDETGAEELLFSFDDVPLIGKHNMENAAFAAGAIRMLT
                     GKGIMRGPLFSGFKGLAHRCEFVANIRGVTYVDDSKGTNVAASATALRSITGPKIVIL
                     GGQGKGEEYAPLAEAVKSEA"
BASE COUNT         1937 a         1890 c         2041 g         2171 t
ORIGIN      
        1 ctaagccatt attttgtttg tgttcttgct tacgttcttg actgtactct tggtctcggg
       61 ggctactcgg aagcgatact cgagtctttc gaatcaacag aggtatatgc cgtagatcag
      121 gacagccaag ccctttcgtt tgcaaaagaa cggcttcttc ctttcggcgc ccgttttcac
      181 cctgaaaaag ggaattttgc ctcccttcct cctgcagtta cttgcaacgc accatttgat
      241 gcgatgttgt tcgatcttgg ggtatcgaac ctccagcttg tcgatgaaga cagaggattc
      301 tcgtttcagt cagacggtcc cttggatatg cggatggacc gagaatttcg ttattctgcc
      361 gccgaccttg tgaactctta ttcagaaaga gagattgcag atatcttctg gaagtacggg
      421 gaggagcgct tttcaagaca gctcgcaaga ggaatcgtcc gatacagaga agaatcaggc
      481 aaaatcgaac gcacgtccac acttgtagat atcgttcgaa aaacattgcc agccccggtc
      541 caacgaaaaa tgggggggca tccggcaaga agaatattcc aagccctacg tatagcagta
      601 aacgacgaaa tctcaatcct cgaaaccggt ttacacaata ttccagatgt cgcagcgccg
      661 ggcgcacttc ttgtcgtggt gagctaccat tctcttgaag acaggattgt aaagaatctg
      721 tttaaaaaat ggcgcgaaga ggggaaagga agtattgtga caaagcggcc tctcgttcct
      781 tcggatgaag agattgaagc gaatcataag tcgcgaagcg ccaaaatgag ggttttttca
      841 ttcaaaccga aaagaatgga ggtgtaaaaa ggacatgcga tttcgcgaag atataaaaaa
      901 agaaaccgtt tctgattcct ttcacaaaat ctctccatat attattgttt ttacgctctg
      961 tgcgtttggc ctctttatgg gactcggagt ccttcgtctt tattctttcc gtctggaatg
     1021 caaactgaat tctgtcaaca ggcaaataga atcatacaaa gcaaatcgta ttgtacttaa
     1081 acatgaactc tcatcacttg tttcccctgc tcgcatctac ggtttttcaa agcaagagat
     1141 gggtatgaca ttcgcatcta atgtccgcat cctccgcgta gaagagcatc ttttagctga
     1201 ggcttctttc gagagttctc ctgttgtcgc ttcgaaacag gagaatgaag gctggttctt
     1261 tttctttctt gaaggcgcaa tggccggaga gtaattcccg tgtatcctga acggagggtg
     1321 tttttgtgac gcgacgtttc ggatcccctt ggtttctagt ttttatcttt tttggcgtcg
     1381 ttatctggag gctgacatct cttcagctct tccctgaccc gagggttgag aatcagtcgc
     1441 gccgacagta ctggattcat gttcctgtca gtaccaatag aggctttatt tatgacagga
     1501 acgggaacgc ccttgcggtt tccgttcctt cattcagttt ttttcttgat cccgcttttt
     1561 ggaatccttc agatgcagta cacctcaaag ggatactccc cgatagttta gtcgatacgc
     1621 tctcgaaacc tctttctggc cgttttttat ggttagcgag aaaagtcgat attgaaacgg
     1681 ctgagaaaat tcgctcgctc aaaattaaag gtatattcga gatagaagag aaaaaaagac
     1741 tctatccgag cggctctctt ctctcacatg tcctcggatt ttgcgatatc gacgacaaag
     1801 ggctttcagg tattgaactc atgtgggata aagcgttgtt ttctcccccg ggtcttcgtg
     1861 tccttgccaa ggattcgtcg ggacgaactc ttgatatcag cagaaatgac catgaccaca
     1921 cacctacgga aggtgggaat gtctccctca caatagactc cagaatccag tttatcgtgg
     1981 agcggcgtct tgaggagggt attcgcgaac atagcgcgcg atggggcagc attatctgta
     2041 tggatcctca aacgggcgcc attctttcga tggcgagttg gccgacgttt aacccaaatg
     2101 tgcgaacgga tctatcgaag acgcagaatt tattaaacaa ttctatcggg cgtacgtatg
     2161 aacccggctc gacgtttaag cccgttgttc ttggaataag catggagaga ggctccgttc
     2221 atccgaatga atcattccac tgcccctata ggatcaaaat tgccgacggt catatatccg
     2281 aagccggaaa aaacggtttc ggaaggcttt ccgtccccga aattctcgca aaatcctcga
     2341 atacaggaat ggcgcaaatt gggatacgcg taaaaccatt caacatgtat cattcagttc
     2401 gcgagtgggg attcggcaaa ccgaccggga ttgaacttcc cggagccgag gacggccttt
     2461 tatcatcacc ggagcagtgg agaggggttg ttccttcgaa tatagccata gggcagggcc
     2521 tcgctattac gccgttgcac cttgcatccg caatctctgc gatagcgaac ggaggcaatc
     2581 tcctgcgtcc acatatcgtt tccaaggtag tagattcttc aggaaatata gtatacaatg
     2641 aagagaggca gtttcttcgg actgtgttgt ctcctcaagt cgcgttatgg cttcagagag
     2701 cgatggtcga tactgttgtc aagggcacag gacggaatgg cgcagttcca ggaatttcaa
     2761 tcgctgccaa gacaggtact gctcagatcg ctgaaagagg agaatacgcc aagggcagat
     2821 gggtttcctc cttcgcaggt ttttggcctt cgagcgatgc tcgctatgtt gtgcttgttg
     2881 tcattggaga accttcaaag ggaaagtatt atggcggaga tgttgcagcg cctgtattta
     2941 ggcgaattgt tgaggacatg gaacagatcg gattgttagc ggcagtgaaa acggaaggat
     3001 gataccttga tgattctctc gaagataatt gaagaatatg ccgcacgtta ttcatctccg
     3061 ttacggctgc ataatcaggc gatcgatgga ggaacggatg tttccgttgt acatgtcggt
     3121 tgcaacagct tggaaataaa gaaagacgag ctgttttgct gcattcgagg cgaacataat
     3181 gacggccatt ccttcgctgg agacgccgtt caaaaggggg cttcaactct tttagtagag
     3241 cgccctcttc cactggaaac acctcaaatc gtgtcatcca acgttcgaag agacatggga
     3301 cgtttatctt caattgtcta cggagatccg gccaacactc tgaaaatgtt cgctgttacc
     3361 ggaacaaacg gcaagagcac gactacgtgg ataatacgcc accttctgca atcaatagga
     3421 atacggacag gccttttagg gacaatagtc tacaatgacg gtattgagga aaaagacgcc
     3481 gcaagaacaa ccccggaaag ctgcgatatt cagctgatgc ttttgagaat gctgcaaaat
     3541 ggctgcggtg cgtgtgttat ggagacatcc tcacatggtc tctctttcgg aagaattgaa
     3601 gggtgttcgt ttgacgccgc agtctttaca aacctcagcg aggagcatct tgattaccac
     3661 ggaacgctcg aaaattactt ttcggcaaag agccgtctgt tttctgacta catgaagcag
     3721 aattgggcgg ggggagcgaa tggtgacgat gcatttgccc gccggctttt aggcctttat
     3781 tccgataagc ttgtttcttt cgggatagag aaagagtatg gcgctcatct ccttggaagc
     3841 aatcttcaga gctctcttga aggaacacgt ttttcgcttt cacttcctgg agtcagtgaa
     3901 cccgttcctg ttttcctgcc tcttccggga aaattcaacg tgtacaatgc tttagctgcg
     3961 ctctctgcaa cgcttccata cgctacggac aaattggctc tcgttgattc cttacattca
     4021 atgattcagg ttcccggaag aatggaaagg tattccttca atagtggagt gagttgtatc
     4081 atcgacttcg cccatactcc tttcgcgcta cagaacgtac tgacggaagt gcgtcgtctc
     4141 tgcggaggaa ggattatttc tgtattcgga cacgggggag aacgtttcca gccgaacagg
     4201 ttttcattgg ggaatatcgc cgctacgctt gcggatgaag tgatcgtcac gatggacaac
     4261 ccgcggaacg aaccggcgga gcagatagcc gcccaaatag tggaaggcgt cgaatcctcc
     4321 gaaagaagga ttcccttgaa aacaattctc gacagatccg ccgcagtgta cgaagctatg
     4381 gattcggcaa aacccggaga tgtcgttgta gtatccggga aagggccgga aaaatacttg
     4441 cgtatcggct ctgaatccat tccatattcg gacggggata ctcttcgccg atgggctgac
     4501 gaaagagggg tagagtggcg atgaaaaacg atcttcgact ttcttgcatt gcggcgcttg
     4561 ccgatactca tctcacaggc cccgacggtc cgatacccct gcgtttcgtt gccgacagca
     4621 gacttgtcgt tcccggggac gcctttgtcg cttttcgcgg cgaacaggtc aacggccatg
     4681 attttattca cgatgcagtt tcgcgcgggg cttctcttat catctgcgaa gaaagcgcgg
     4741 gagtaccgaa tcatgtcgct tccatcgtcg tggacaactg tttttcggca atacctcgaa
     4801 tggcgcgcag atacctcgaa attcaccgcg aatcactcga ggtacttgcc gtcaccggaa
     4861 gtgtcgggaa aacaacgacg cgtgagatga tccgcacctg cctggctccc tcgtttaaag
     4921 tacattcagc ggaacacagt tacaacacgc ttattgggtg cgcgctctcg atacttgcca
     4981 tgccggcgga caccgaaata ctgatactgg aaatgggcac caaccatccc ggcgagattg
     5041 gagagatggt tgatttcttt cctccgacga tttctatgat taccgaggtc gctcccgctc
     5101 atcttgaagg actgggcagt gtggaaggtg tggcacaggc aaagttcgaa attaccggat
     5161 ctccttttct taaggctttc ttttatttcg gagacaaccc gctgcttgtt tctggagcag
     5221 cttcgcttcc ttctcatgtg cgttctctca gcgtaggctt cggggagaat gacttttcaa
     5281 tccgtgatac ggaatttatt ctctccaacg gactgccgag gctcactttc gacgtagtct
     5341 acaagggaaa aactttccac gcatcatctc ccctttttgg acgacattcg gcgttggcgg
     5401 cgggattcgg aatcgcattg tcactttatt taggggcgtc tattgaacaa gcccttcagt
     5461 ctattgaatc tctccagccc ccctgcggaa ggggccggtt ttttcttctg acttcgggag
     5521 ctatgcttct tgacgactcc tacaacgcaa atccggcttc gatgaaggcg tcgctctccg
     5581 aatcgcttcg tattccggtg cagcgtcgta tcgccgtact tggtgaaatg aaggagcttg
     5641 gaaaaagtgc tctggactat catcgagaac tgcttctgtt ttttaccaaa ttttccaagg
     5701 tgttcattat gggtacgctc tggcggactc tgttccaaaa tacaccgctt cccgataata
     5761 ccgttttctt tgataatccg ctctcgctcg gaaccgagat aaagaaagaa cttggcgaag
     5821 gagatctcat tgtagtcaaa ggatcgcatg gtaacagact ggattttttg gtcgatttcc
     5881 ttgtcgagga gggagtggaa tgatttacgc tctttcctgg gctttcctct tcttcttgca
     5941 gatattctgt tctaagatct ggatcgccag gcttcatcaa tggcgcgttg tgcagattca
     6001 aaaaagctac gggttgcaaa gccacatcga aacgaaatca tcgacgccat ccatgggtgg
     6061 agttgtgttt ctcctcatag gaattgcgag tcttctgccg gaagcagttt tttctgagac
     6121 gcgcttatct aacgcggcac tcttctggat tctgcctttg ggatccgcag ctatcggttt
     6181 tattgacgat tggatcaaac ttaccaggaa atccagcgaa ggcttcccta gcctctataa
     6241 acttgccgca cagatactgc ttacagtacc gtggtcgtgt tggattgtct tttcacaagg
     6301 cctctctctc tggccgggcg tcgttgttcc ggctctcctc gccgtcccgc ttctgacgat
     6361 agtatcaata gggatgttaa atgcggtgaa tgttaccgac ggcctggacg ggcttgccgc
     6421 cggtgcggcg ctgttttcat tcattggagc gatcgttttg ctgccccttg agagttttct
     6481 gctatatcct gctctggcgg gttctgctct ctgtgccggg ttcctttggc ataatgcgca
     6541 cccggctaag gtgttcatgg gagatatagg ctcccacttt ttggcgggta tgctcgttgc
     6601 cctctgtgcg cacagtgaat acttcattgc tgttatccct ctcggttttc tctttggcgt
     6661 ggagatagtc tcagttgcaa tgcaactgat cgccattcat tgtttcaaga gaaaggtctt
     6721 tcggatgagc ccgcttcatc atcatttcga actccttgga tggagcgaaa cgcagatagt
     6781 cactcgtttt tggataatcc atggagcagg acttgcgttc cttgcacttt ttgtacaggg
     6841 cattctttgg aaggcggtgt gagatgatgc cgttggacgt ggacagtttc tcaggaaaaa
     6901 gagtttccgt catcggggcg ggaatcagcg gagcaggttt ggctcgtttg gcaaacctgc
     6961 tccgcaggtt tggctcgctt ggctcatcgt cttggagcgc aagtttttgt ctcggatgaa
     7021 aaaaaactgc aggaccaatc cctcgtcgct ctttttcagt ctcatgggat tgagtgggag
     7081 gatggaggac acaccccccg tctttttgaa gcagattata ttttggtagg ttcaggtatt
     7141 tctcccaacg caaaacctgt gacggagagt aatcgaagga atatcccagt gataggggag
     7201 ttggatttta tatcgccgtt tctccgaagc agaattattg gtgtaaccgg aagcaacgga
     7261 aaaagtacaa cgaccgcgct tgccggctat cttttacaga aagcgggctt ctccgcagaa
     7321 gttgcgggca acataggaaa atcgctagcc gactttgcct ttgtgcaacg cgactttctc
     7381 gtggtggagt tgagcagttt tcaacttcac tggaacacca gatttgcatg cgatcttgcc
     7441 atggtgacaa acctggctcc tgatcatatc gactggcacg gcagttatga agagtacatt
     7501 cgggcaaagg caaagatcct ctcaacacaa tccccgggag gcttcgctat tgttcaggaa
     7561 acggaacgca gcgtcctttt cggagatcgc gaggatgacg ctcacgaagt tctagctttc
     7621 tcgtggaatg gaatgcaatc atctcatgga agaaaacttc ttgctgacga aaaagcatgt
     7681 catgtactat tccgggacga aacaggagcc gaggaactcc tcttctcttt tgacgacgtt
     7741 ccccttatag gaaaacataa catggaaaat gcagcatttg cagccggagc tatacgtatg
     7801 ctcaccggaa aaggtattat gcgcggtccg cttttttcag gttttaaagg gttggcacat
     7861 aggtgcgagt tcgtggcgaa cattcgcggc gtaacctatg ttgatgattc gaaaggaaca
     7921 aacgtagcgg cgagcgctac cgctctcaga tcaataacag gacctaaaat tgttatactg
     7981 ggcggtcagg gaaagggaga agaatatgcc cctttggcag aagcggtaaa aagtgaagc
//
LOCUS       DAOPKU010000064         8021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9549_length_8021_cov_4.404846, whole genome shotgun sequence.
ACCESSION   DAOPKU010000064 DAOPKU010000000
VERSION     DAOPKU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9549_length_8021_cov_4.404846"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK849_03160"
     CDS             <1..708
                     /locus_tag="PK849_03160"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPE65154.1"
                     /translation="GPLCIIDEGAVIEERAFLHGRVFIGRDCCVGAETTVEPGAVLFE
                     SVRTGRRCLIHSNAVLGCDGFGITPSAGEAPPEKIPQIGGVVLGDDVELGVCSTVDRG
                     TLDDTVIGNGTKVDNHVHIAHNVRIGRNCILVAMTGIAGSAILEDNVIMAAGSGVKEH
                     TRIGKGATVAAKGGAIKDVPPGEVVSGFPARPHRENFRVQAALQRLPETLSRVKKLEK
                     RLDALLPDGSGGSGAGE"
     gene            705..1574
                     /locus_tag="PK849_03165"
     CDS             705..1574
                     /locus_tag="PK849_03165"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPE65155.1"
                     /translation="MTATRRLLHPVCFSGQGLHTGESSEVVISPLPSGSGPELEIEGA
                     RTPLEICPVSGAGRGTTVSLPHSDLRARTTEHLFAALAVLGLWSVRISLKGPEIPAAD
                     GCSALFAESLYGASAPEKPEEDSSMRMDAFSPAFPVTIVDGEKESSLSLFPWGALSIT
                     YVIQYAERPIGTQMATFDAEKDDFRKYIAGARTFALDTEIERLRAAGLARGGSLDNAI
                     VVGEDAVQVLGGLRFPDEFVRHKILDLLGDLYLLGRPLNARITAIRAGHAMHCILVDR
                     IRRLCRGGRRANQ"
     assembly_gap    1650..1749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1750..1953
                     /locus_tag="PK849_03170"
     CDS             <1750..1953
                     /locus_tag="PK849_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65156.1"
                     /translation="RPVKPGDQLVTTAEMLKLRGKMGKVRTVGRVDGAVAAEAEFSFV
                     AAETLAASEEKAAPSRSGEEAAR"
     gene            1954..2775
                     /gene="lpxA"
                     /locus_tag="PK849_03175"
     CDS             1954..2775
                     /gene="lpxA"
                     /locus_tag="PK849_03175"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPE65157.1"
                     /translation="MPLIHPTALVSPKAELADDVVVGPYCIVHDRVRIGSGTVLSSFV
                     TLLDFVETGKNCRFEQHVVLGGEPQDHAFNGEESWVRIGDAVTLRENVTVHRATGEGA
                     CTVVGDESLLMEGVHVGHNAVVGKRATLANKVGLSGFSSVGDGAVMGGMSGIHQFVRV
                     GALCMIGGLSKIVKDIPPYLMADGHPADIYGLNKVGMRRAGFDASARSAVRSLYDELF
                     RGGLPFRQAVERLAASEKGSDPVAREILSFCGESKRGVALWTKGHAFRGKDHEED"
     gene            2762..3271
                     /locus_tag="PK849_03180"
     CDS             2762..3271
                     /locus_tag="PK849_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPE65158.1"
                     /translation="MKRIKLLVLDVDGTLTDGGVYMDGRGGEFKRFDIHDGMGIVRLR
                     AAGVRTAFLSGRRSEATAQRAADLGVELLFNGVREKLPVLCSLLSDLSLSAEEAAYMG
                     DDVNDVDCLRAAGLAFAPADAVPEAKAAADFVTSAKGGGGAVREAAEHIISLNARCEG
                     RSSGAEERP"
     gene            3271..4377
                     /locus_tag="PK849_03185"
     CDS             3271..4377
                     /locus_tag="PK849_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPE65159.1"
                     /translation="MRKRAGVGILDRFILKELSGSFLFGVTSFTMVFVAGDLLFQAAN
                     LIIEQGVSLGVVARLFIYRLPEVVMLTLPMASLLSSLLTFSRLSTNSELVALKSAGIP
                     FHRILRPVFLASILVSLASTIGAETVVPFANRAAENLMKFEILQEKPSLLKEKVFLRE
                     EKDGVLQRVLYLGELKPKDGLMKDIIIQEFEEGRLRRISLADEGLWAGGEWWIENGQV
                     FEVTPAGKVQTLFRFERQRLILNLSPVQIEQASRRPSEMSSLELLAQIALMEQQGQNL
                     APLKVMFHLRLALPWASVVLAVLGSSLGVRSTRAGPGIGFGLSVLIVFAYYVVMSFSR
                     ALGEAGYLPAFIASWFPNMSFLLVGGYFARRANG"
     gene            4462..5406
                     /gene="lpxI"
                     /locus_tag="PK849_03190"
     CDS             4462..5406
                     /gene="lpxI"
                     /locus_tag="PK849_03190"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583133.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HPE65160.1"
                     /translation="MKQDVSPCSPSGNSAGALIAGEGALPLLVAEYASRSGMQLTVYC
                     LGDRSAYVGLYGVEAVPLPSCEGNDSDPRGGLDLRATIQDMLRRGVGAVTLAGTVPKR
                     MMYEARLDPSLRALLAGGKNDDHSLLGRIVGAFESAGLRVLPYTSYLADSLAPEGHVA
                     GRDPSDAEAADIEYGRGVLSVTLPLSFGQSVVVAGGAVVAVEAMEGTDAMIRRAGELL
                     GGRSAKAGVDVPVPATGGGVVVKMMRCDQDERYDVPVVGLSTLETMRDAGVSCLAVEA
                     GRTLLLHPETFREHAAMWRIAVVGISGGSKPLSGGPRS"
     gene            5403..>5845
                     /locus_tag="PK849_03195"
     CDS             5403..>5845
                     /locus_tag="PK849_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPE65161.1"
                     /translation="MTAEMAAGGLFISCGELSGDGYASAVVRTLRSKGFGGRVFGMGG
                     TRSAAAGVEILWDSRMLHLMGIGEVLPSIPRLLRLKSRMIDTVMFSGVPTVLVIDSPD
                     FHLPFVASLRARGWRGRVVYAVPPTVWAWRSGRVKALARDVDLCLP"
     assembly_gap    5846..5945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5946..6395
                     /locus_tag="PK849_03200"
     CDS             <5946..6395
                     /locus_tag="PK849_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65162.1"
                     /translation="ELRGMEIFEGEGRELLAGSAAAAGASGTVAVEAMLLDRFMVVLY
                     KTGFLSECVWRMLGKTERISLPNLLAGRDVYPELLQRHATPKNAMEALLRYLRDPEYR
                     DAVHSGLRSAREGMGEPGALAFWAESILSGEVETQRPERQRTEEISP"
     gene            6392..7564
                     /locus_tag="PK849_03205"
     CDS             6392..7564
                     /locus_tag="PK849_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPE65163.1"
                     /translation="MSRKIALLTNGPGELWGWVRPFAAELLRRRWDVSLRILPCQFAS
                     GEERRIALALGIPDVTGPESSLRTALSLRKFGKGANGTPPDAVVQLGGDLLWGRMLAA
                     SARAPLFCYSYGRKNGIERCAALFTAYREMAESIASSGEGQALQRTGRILLAGDLAAE
                     LSDTFVHAPDGEDRIPEGRPRVAFFPGSREHIREYAVPLIRELAAALRESFPALDARI
                     ILSPFASAGEEEAFRRSGDLTPVRGGSKESLRGLDFAVTQPGTNTLELMHCAVPFLVA
                     VPFSSLRHIPLPGVAGALANIPLIGPALREAALKAKGRRTGFVAWPNRLAGRQVVDEL
                     IGDISARDVARVVGGHLLDGEYLARTKKSLAALSSSAPRNAAVFMADHIERMLAER"
     gene            7561..>8021
                     /locus_tag="PK849_03210"
     CDS             7561..>8021
                     /locus_tag="PK849_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPE65164.1"
                     /translation="MTSEEKTASPSPYLRLLAYARPYSRRFFLALGCMVAASACNVVP
                     PWLLKNVVDDVLIAKNLAMLNALPFILVALFAGKGIASYGHQYLMNWVGQNVVMDLRL
                     RLYDHLQRMSLKYLYGKRLGELMSRITNDVTILQNLVTSVLVNLVVQAATF"
BASE COUNT         1359 a         2274 c         2580 g         1608 t
ORIGIN      
        1 ggtcctctct gtataattga cgaaggcgcc gtcatcgagg aacgcgcttt tttacatggc
       61 cgggttttta tcggcagaga ctgctgtgtg ggggcggaga ccacagtcga gccgggggcg
      121 gtcctctttg aaagcgtgcg aacaggacgc cgctgcctga ttcactccaa tgcggttctc
      181 gggtgcgacg gattcggtat tactccctcc gcaggcgagg cgccgccgga gaaaattcct
      241 cagataggcg gcgtcgtcct cggagacgac gtggagctgg gcgtctgctc aacggtggac
      301 aggggaacgc ttgacgatac cgtaatcggg aatggtacga aggtggataa ccatgtgcac
      361 atcgcgcata atgtccgcat aggcaggaac tgtattctcg tcgccatgac gggaatcgcc
      421 ggcagcgcca tccttgagga caacgtgatc atggcggccg gcagcggggt gaaagagcat
      481 acgcggatag ggaagggcgc tacggtcgcg gcgaaaggcg gagcgatcaa ggatgttcct
      541 ccgggggagg tcgtctcggg ttttccggct cgtcctcaca gagagaactt cagggtacag
      601 gcggcgttgc agcgccttcc ggaaacgctc tcccgtgtaa agaagctcga aaaacgcctt
      661 gacgcgctgc tccccgatgg gagcggcggg agcggagcag gggaatgacc gcgacccgcc
      721 gtctgcttca tccggtctgc ttctccggac aagggctcca tacaggagaa tcgtcggagg
      781 tcgtcatctc tcccttaccc tccggttccg gacctgaact ggagatagaa ggcgcaagga
      841 cccctttgga gatctgtccc gtttccggcg ctggacgcgg aacgacagtt tcccttccgc
      901 acagtgatct acgggctcgt acgaccgagc atctctttgc agcgcttgcc gtgttggggc
      961 tctggtcggt gcggatatcg ctcaaggggc cggagattcc ggcagccgac ggatgctccg
     1021 ccctcttcgc cgagtcgctc tacggagcgt cggccccgga aaaaccggaa gaggattcgt
     1081 ccatgcgaat ggacgccttt tctccggcgt ttcctgtgac aatagttgat ggcgagaagg
     1141 agagctctct ctctctcttc ccttggggag cgctttcgat cacgtatgtc atacagtacg
     1201 ccgaacggcc gatcggaacc cagatggcga cgtttgacgc agaaaaagac gacttccgga
     1261 aatacatcgc aggagcgcgg accttcgcgc ttgatacgga aatagaacgc ttgcgcgccg
     1321 ccgggcttgc ccgcggcggg agtcttgaca acgcgatcgt cgtgggagag gacgctgttc
     1381 aggttttggg aggcttgcgt ttccccgacg agtttgtccg gcataagatt ctcgacctgt
     1441 tgggcgacct ttatctgctc ggacggccct tgaacgcccg aattacagcc atccgggcgg
     1501 ggcacgcaat gcactgtatt cttgtggaca ggattcgcag gctctgccgg ggcggacgca
     1561 gagcaaatca atgaattttt tatgaaaagg agacgaacta tgcttgatat ccagaagata
     1621 atgaaatttt tgccgcaccg tttccccttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnna ggcccgtaaa gccgggagac cagcttgtga cgactgcgga gatgctcaaa
     1801 ctcaggggga agatggggaa ggttcgcacc gtaggtcgtg tggacggagc agttgcggcg
     1861 gaggcggaat tcagttttgt ggctgccgag accctcgccg cctcggaaga gaaagccgcc
     1921 ccctcgcgaa gcggggagga ggcggctcgt tagatgccgc tcatccatcc gacggctctt
     1981 gtttcaccga aggcggaact ggcggacgac gtcgtcgtcg gcccctactg catagtgcat
     2041 gatcgcgtcc ggatcgggtc ggggacagtg ctctcatctt tcgtcaccct gctcgatttc
     2101 gttgagacgg ggaaaaactg ccgttttgaa cagcacgtcg tcctcggcgg cgagccgcag
     2161 gatcatgcgt tcaatggaga ggagtcgtgg gttcgcatag gcgacgccgt gacgctccgt
     2221 gaaaacgtca cggtgcatcg cgctacgggc gaaggcgcct gcaccgtcgt cggcgacgaa
     2281 tcgctgctga tggaaggagt ccacgtcgga cacaatgccg tcgtcgggaa gcgcgctacc
     2341 ctggcgaaca aggtcggcct ctccggattc tcctccgtcg gcgacggcgc cgtcatgggc
     2401 ggtatgtccg gtatccatca gttcgtccgt gtcggggctc tctgcatgat cggagggttg
     2461 tcgaagattg tcaaggatat tcctccctac ctcatggcgg acggacaccc tgccgacatc
     2521 tacggtctga acaaggtggg gatgcggcgg gcgggcttcg acgcgtccgc acgttccgca
     2581 gtccgctccc tctacgatga actcttcaga ggaggcctgc ccttccgcca ggccgtcgaa
     2641 cgcctcgccg cttccgaaaa gggaagcgat ccggttgcca gggagattct ctccttctgc
     2701 ggggaatcca agcggggggt tgcgctctgg acaaagggac atgccttccg ggggaaggac
     2761 catgaagagg attaagctgc ttgttctcga tgtcgacggc acgctcaccg acgggggagt
     2821 gtatatggac ggccgcggcg gagagttcaa gcggttcgac atccatgacg gaatgggaat
     2881 tgtccgtcta cgcgcggcgg gagtccggac ggctttcctc agcggacgcc gttcggaagc
     2941 gacggcgcag cgcgccgccg atctcggcgt ggagctcctt tttaatggag taagggagaa
     3001 acttcccgta ctatgttcgc tgctgtcgga tctttcactt tccgcagagg aggcggccta
     3061 catgggggac gacgtgaacg acgtcgactg tctccgggct gccggtcttg cttttgctcc
     3121 tgcggatgca gtcccggagg cgaaggccgc ggcggatttc gtgacgtccg ccaagggcgg
     3181 aggcggggct gtccgcgagg cggcggagca tatcatttcg ctgaatgcac gctgcgaggg
     3241 gcgttcttcc ggagctgaag agagaccgtg atgagaaaaa gggccggggt cggtattctt
     3301 gatcgtttta tcctgaagga gctttccggc tccttccttt tcggcgtgac ctctttcacg
     3361 atggtctttg tcgccggaga tctgctcttc caggcggcga acctgatcat cgagcagggc
     3421 gtttcgctcg gcgtagttgc ccgcctcttc atctaccggc ttcccgaggt ggtgatgctc
     3481 acgctcccca tggcctctct gctctcgtcg ctgctgacgt tcagcaggct ttccaccaac
     3541 agcgaactgg tcgccttgaa gtcggcgggg ataccgtttc acaggattct tcgtccagtc
     3601 ttccttgcct ccatcctcgt gagtcttgcc tcgacgatcg gggcggagac tgtcgttccg
     3661 tttgccaaca gggcggcgga gaacctgatg aaattcgaga tcctgcagga gaaaccgtcg
     3721 ctgctgaaag aaaaagtctt tcttcgggaa gaaaaggacg gtgtgctcca gcgggtgctc
     3781 tatctcggag agctcaagcc gaaagacggt ctcatgaaag acatcattat ccaggagttc
     3841 gaggaggggc gtttgcgaag gatctccctg gcagacgaag gattatgggc agggggcgaa
     3901 tggtggatag agaacggcca ggtgttcgag gtaacgcccg ccggcaaagt gcagacgctc
     3961 ttccgctttg aacggcagcg cctcatcctt aatttaagtc ccgtgcagat agagcaggcg
     4021 tcgagacgcc cttcggaaat gagctctctg gagcttctgg cgcagatcgc gcttatggag
     4081 cagcaggggc agaaccttgc gccgctgaag gtgatgtttc acctccggct ggcgctgccc
     4141 tgggcgagcg tcgtgcttgc ggtgctgggg tcgtcgctcg gcgtccgctc cacgcgcgca
     4201 ggtcccggaa tcgggttcgg actcagcgtg ctcattgttt tcgcctacta cgtcgtgatg
     4261 tccttcagcc gggctctcgg cgaggccggg taccttccgg cgttcatcgc ctcgtggttt
     4321 ccgaacatgt cgtttcttct cgtcggcgga tactttgcca ggagagcgaa cggctgattc
     4381 cgagcgcgtc ccgccgcgga ctgctcgttg ctacggagcg ttccggcagg gacgaatgat
     4441 atcgtgaaag ggttggaata tatgaaacag gacgtttcgc cgtgctctcc ttccgggaat
     4501 tccgccgggg cgcttattgc cggagaaggc gctctgccgc ttctcgtcgc tgaatacgcg
     4561 tcgcgctccg gtatgcagct cacagtctac tgcctgggcg accggtccgc atacgttggt
     4621 ctgtacggag tggaagccgt cccgctgcct tcgtgcgagg ggaacgattc ggacccccgc
     4681 ggaggactcg acctgagagc gacgatacag gatatgctcc gccgcggcgt cggcgccgtg
     4741 acgcttgcag gcactgtgcc gaagcggatg atgtacgaag cgcgcctcga tccctcgctc
     4801 cgcgcccttc tcgcaggcgg gaagaacgac gatcacagcc tgctcggaag aatagtcggc
     4861 gcctttgaaa gcgcggggct gcgcgtgctt ccgtacacgt cgtaccttgc agacagcctt
     4921 gccccggagg ggcatgtcgc cgggcgggat ccgtcggatg ccgaggctgc ggatatcgag
     4981 tatggccgcg gcgtgctctc cgtcaccctg ccgctctctt tcggtcagtc cgtcgtggtt
     5041 gccgggggag ctgtggttgc ggtggaggcg atggagggga cggacgccat gatccgcagg
     5101 gccggggagc ttttaggcgg caggagcgcg aaggcgggag tcgacgtgcc cgttccggct
     5161 accggaggag gcgtcgtggt gaagatgatg cggtgcgatc aggacgaacg ctacgatgtg
     5221 cctgtcgtcg gcctctccac gctggagacg atgcgcgacg ccggagtgtc ctgccttgcc
     5281 gtggaggcag ggaggacgct gctgctccat cctgagacat tcagggagca cgcagcgatg
     5341 tggcgtatag cggtcgtcgg gatatcgggc ggttcgaagc cgctttccgg aggtcctcgt
     5401 tcttgaccgc ggagatggcc gccggagggc ttttcatcag ttgcggcgag ctttccggcg
     5461 acggatatgc ttccgctgtc gtgcggacgc tgcgctccaa ggggtttggc ggaagggtct
     5521 ttggaatggg cgggacgcga agcgccgctg caggtgtgga aatcctctgg gactcccgta
     5581 tgctccacct catgggtatc ggcgaggtgc ttccctcgat cccgcggctg cttcgtctga
     5641 agtccaggat gatcgatacc gttatgttca gcggagtgcc aacagttctc gtgatcgaca
     5701 gcccggattt tcatctaccg ttcgttgctt ctcttcgtgc ccgcgggtgg cgcggacgcg
     5761 tcgtgtacgc ggttcctccc acggtatggg cgtggaggag cggccgagtg aaggcgcttg
     5821 cccgcgacgt ggatctctgc cttccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnngagct tcgaggcatg gagattttcg agggggaggg gcgcgagctc ctcgccggga
     6001 gcgccgcggc cgcgggagcc agcggcactg tcgccgtcga ggcgatgctg ctcgaccggt
     6061 ttatggtcgt gctgtataaa acgggtttcc tttcggaatg cgtctggagg atgctgggga
     6121 agacggagag gatctctctg cccaatctcc tcgccgggag ggatgtctac cccgagctgc
     6181 tgcagcggca tgcgacgccg aaaaacgcga tggaagcgct gctccgctac ctccgcgacc
     6241 ctgagtatcg cgacgccgtt cacagcggcc ttcggagtgc cagggagggg atgggagagc
     6301 ccggagcgct tgcattctgg gcggagagca ttctgtccgg cgaggtcgaa acgcagcgtc
     6361 cggagcggca gcgtaccgag gagatatctc cgtgagcagg aagatcgctt tgctgaccaa
     6421 cggccccgga gagctctggg ggtgggttcg ccccttcgcc gcggaactgc ttcgccggcg
     6481 ctgggacgtg tcgctcagga ttcttccctg tcagttcgcg tcgggggaag agcgccgcat
     6541 cgcgcttgct ctcggtattc cggatgtaac cggaccggaa tcgtctctcc ggacggcgct
     6601 ctccctgcgg aaattcggga aaggggcgaa cggaacccct cccgacgcgg tggtgcagct
     6661 cggcggcgac ctgctctggg ggcggatgct cgccgcgagc gcccgggcgc cgctcttctg
     6721 ttactcgtac gggcgaaaaa acgggataga acgctgcgcc gccctcttta ccgcctacag
     6781 agagatggcg gagtcgatcg cctcctccgg cgaggggcaa gcgctgcaac ggacagggcg
     6841 tattctcctg gcgggcgatc ttgccgccga gctgtcggat actttcgtgc acgcgcccga
     6901 cggcgaagac cgcatacctg agggacgtcc aagagttgcc ttcttccccg gaagccggga
     6961 acacatcagg gagtacgccg tgccgctgat acgcgagctt gctgcggcgc tgcgagagtc
     7021 gttccctgcg cttgacgccc gcattattct ttctcccttc gcttcggccg gggaggagga
     7081 agcgttccgt cgctccggag atctgacccc ggtgcgagga ggaagcaaag agtcgctccg
     7141 gggccttgac ttcgctgtga cgcagccggg aacgaacacg ctcgaactga tgcactgcgc
     7201 cgttcccttt ctcgtcgcgg ttccgttttc ttcacttcgc catattccgc tgcccggcgt
     7261 cgccggagcg ctggccaaca ttccgctgat cggccctgcg ctgcgcgaag ccgcgctcaa
     7321 ggcgaaaggg cgccggacgg ggtttgtggc ctggccgaac cgcctcgcag ggcgccaagt
     7381 tgttgatgaa ctgatcggcg atatctctgc gcgggatgtt gcccgggtcg tcggagggca
     7441 tcttctggac ggagagtacc tcgcccggac gaagaagtcg ctcgccgcgc tctcttcctc
     7501 cgcgccccgc aacgccgccg tattcatggc cgatcatatt gaaaggatgc ttgctgaacg
     7561 atgacctccg aagagaaaac cgcctcccct tctccgtatc ttcggctgct cgcctacgcc
     7621 cgtccctact cgcgcaggtt cttccttgcg ctggggtgca tggttgcggc ctcggcctgc
     7681 aacgtcgtgc cgccgtggct gctcaagaac gtcgtggacg acgtcctgat cgccaagaac
     7741 cttgccatgc tcaacgcgct tcctttcatc ctcgtggcgc tcttcgccgg gaaagggatc
     7801 gcctcgtacg ggcaccagta cctgatgaac tgggtagggc aaaacgtcgt gatggattta
     7861 cgccttcggc tctacgacca tctccagcgg atgtcgctga agtacctcta cggcaagcgc
     7921 ctcggagagc tgatgtcgcg tatcacgaac gacgtgacca tcctccagaa tctggtcacg
     7981 tccgtactgg tcaatcttgt cgtgcaggct gcgaccttct t
//
LOCUS       DAOPKU010000065         7949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9683_length_7949_cov_5.243096, whole genome shotgun sequence.
ACCESSION   DAOPKU010000065 DAOPKU010000000
VERSION     DAOPKU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9683_length_7949_cov_5.243096"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PK849_03215"
     CDS             complement(<1..573)
                     /locus_tag="PK849_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE65165.1"
                     /translation="MSDYSITILNFIAIQAIVACGLNVIVGYAGQISLGHAAFFGIGA
                     YSSALLTTKAGLTFWAALPLVVLITAAIGLVLGMPSLRLREDFLAVTTIGINFIVESV
                     FLYVPFFGGALGLGGIPSIVFFGTRLRGANFLYLCLFFLALVLLISRRFTKSWAGLAC
                     FALREEETAASSMGVSPVRFKLMAFVIGTAM"
     gene            complement(587..1450)
                     /locus_tag="PK849_03220"
     CDS             complement(587..1450)
                     /locus_tag="PK849_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE65166.1"
                     /translation="MQLFIEQLLNGAAMGAIYALITLGLALVYGVLRILHVAHAGVYA
                     AGAYAGLYVYSHTGSLLLAVLGAMIFCALLGVLIERLIYTPLLKFPPFVPLIGSIAVF
                     LALEEIFRLVGGPYILSFPAELPFSGITVSGVVISPAIISIYAVSASVLLLLWFIVTK
                     TEFGLAMRATSQDREVAGAMGIDGAFTVSMTFALGSAVAAVAGILVGIYFNQVYPTMG
                     AVPAYKCLALIVVGGLGSLPGAVLASLLLGVGETLLIGYANIPLPRDSLAFIAMIAVL
                     LWRPGGLMGNK"
     gene            complement(1571..1750)
                     /locus_tag="PK849_03225"
     CDS             complement(1571..1750)
                     /locus_tag="PK849_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65167.1"
                     /translation="MKALVTGFDPFGGVKLRAEHRGHSATRFAAEQAGRNWLKALFLR
                     RPERGECVYTLPGLF"
     gene            complement(1747..2979)
                     /locus_tag="PK849_03230"
     CDS             complement(1747..2979)
                     /locus_tag="PK849_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE65168.1"
                     /translation="MKPGKGFADFFGLKKSIVGMLSMVILVGLGERMAERFLPIYLLT
                     LGGGALSIGMLNGLDNLLSALYSFPGGYIADRFGTKRALLFFNILAMTGFAVVIFVPR
                     WEAVIGASFLFLSWTAISLPATMKLIASALPKNKRTMGVSVHSLVRRIPMALGPIVGG
                     AMIGLFGESLGVRIAFGGAFFFALLALLLQQRLIDDAHQDVVERPEGNPLKIFRKMSP
                     ELKNLLVSDILVRFCEQIPYAFAVVWAMKTIASPVTAFQFGLLTAVEMVVALLIYIPV
                     AWLADRGRKKIFVTITFCFFTAFPLALLASRSFEALLIAFAVRGLKEFGEPTRKSLIL
                     DLSPHGESAAMFGLYYLLRDTVVSVAAFGGAFLWMAGPAVNFLTASAFGAAGTIWFIV
                     YGKDLPSGEAVAKGDGAG"
     gene            complement(2982..3977)
                     /locus_tag="PK849_03235"
     CDS             complement(2982..3977)
                     /locus_tag="PK849_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HPE65169.1"
                     /translation="MDHSLLHIISHTDLDGIAAAAVAWHRWQPTRPLKVSLAGYGTVD
                     ALILESIEAGQEFVVIDLFCQNEKTIEKLDKQYIADDAPFLFDHHDTTAARYGNRPWA
                     VVDTNYCAAKVYYRWLVGTGAPGMERLAPLVELANDRDLWINENPDSRLWQALITLCG
                     PQSLFARLAANPEAVLLPHERAAAVEFVEKQEKRFALAVEKIGKSKDDLAFVEPGILE
                     FGDVSDFGGLVLDRMANPPLLVAVAAKRYSGEWAVSMRSRSETAGKVVGMLRDGKKIR
                     GGGHDDSAALYFPPSYSPEQIRTTITAALRTIKDSERPTGVTLGDLLASGKSGRK"
     gene            4484..5812
                     /gene="dnaA"
                     /locus_tag="PK849_03240"
     CDS             4484..5812
                     /gene="dnaA"
                     /locus_tag="PK849_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPE65170.1"
                     /translation="MVDNDLQHLWDHILDEAGSQLPAGATETWLRTCAPTEFTDGVLV
                     LDVPNVFVKEQISSRFLKELIHIFTETGGGRSIELRVGAETRKEERERAERAVSAGNL
                     ESRSGLNPGYLFNSFVVGKSNRLAHAASLAVAETPGEAYNPLFIWGGVGLGKTHLMHA
                     IGHYVLDRNSNSRVTYVSSEKFINEFIQSIKNNKTQEFKSKYRSVDILLIDDIQFLGN
                     KGSSQEEFFHTFNQLHTSKKQIIICSDRPPKEIQSIEDRLVSRFEWGLVTDIQSPDLE
                     TRIAILQKKAQLRRYNIPDDVINFLAINIPSNIRELEGALNRVVACAELAAEPINEEN
                     TREWLKDVIRKDLRGPVSIDSIQNTVADFFSMSVDELLSSKRTSDLAMARQIAMYLCR
                     KITESSLQQIGMAFKKKDHTTVLHAHRKIAQMIKEQPRIKQIVDTIEGKL"
     gene            6040..7218
                     /gene="dnaN"
                     /locus_tag="PK849_03245"
     CDS             6040..7218
                     /gene="dnaN"
                     /locus_tag="PK849_03245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPE65171.1"
                     /translation="MRLQISKGEFMKNWQIAERSTSSKSTINSLTGVLLRTADADNSV
                     LMEATDLKTSIKCISQGVLVEEEGEAVLPVKLVGELFKKAPTNVFTVSITDGKGIIIA
                     GRNKYKFTTYSPREFPKLPVSESAAFFCTATASELLRAVSEGTVACTTGEEFPKYLGT
                     AFFQLRDGEMRVVATDGRRLSLSKCSPVRTGENCDFLLPITGVRELQRLLGALDGESP
                     IEILVESTLVFFQMGSMEFSVRRVDTAFPNYENILNPQTTTTMDIDRHELIAALERVD
                     VIVRDFSHMVLVRLSPEGDLLLTGKAPETGAVQEVLDAKIEGESLTVAFNVSFLIEGL
                     KALYGDRVFISFNGPDGQMTMLRPGEKDFLYMAMPIKINESDLLHGEMDEENETEIPE
                     "
     gene            7228..>7949
                     /locus_tag="PK849_03250"
     CDS             7228..>7949
                     /locus_tag="PK849_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE65172.1"
                     /translation="MRCGALRLRNFKNITTETILWGEGLNLLVGKNGAGKTNCLEALH
                     LLLGWGPLGDRKDIASWGSAEKKTYVTGDFFGEEEAFLAVGIGGSTVVKYNGKRCSFP
                     EVRSVAPSLAFLPSDMELLDGSPARRRSFLDRLCALLWPLYARRLVEFRRAVRHRIVL
                     LRQGGNLVAISKAMAPLASWLWGARASAVSALAEGLAALPDLLPLPVRLAHVRGGAGR
                     VKDPLEDWWESLEARREKERLC"
BASE COUNT         1700 a         2367 c         2482 g         1400 t
ORIGIN      
        1 catcgccgtg ccgatcacga aggccatcag cttgaagcgc accggcgaaa cccccatgct
       61 ggaggccgcc gtctcctcct cgcggagggc gaagcaggcc agtccggccc agctcttggt
      121 gaagcgccgc gagatcagca ggacgagcgc gaggaagaag aggcagaggt agagaaagtt
      181 cgcccccctg agcctcgtgc cgaaaaagac gatgctcgga atgccgccga ggccgagcgc
      241 gccgccgaag aacgggacgt acaggaagac ggactcgacg atgaagttga tgccgatggt
      301 ggtgacggcg aggaagtcct cgcgcagacg gaggctgggc atcccgagga cgagcccgat
      361 ggccgcggtg atgaggacca cgagcgggag cgccgcccag aaggtgagcc ccgccttggt
      421 ggtgaggagc gccgaagagt acgcgccgat accgaagaag gcggcgtgcc ccagcgagat
      481 ctgcccggcg taccccacga tgacgttgag gccgcaggcg acgatggcct gtatcgcgat
      541 gaagttcaga atggtgatgc tgtagtcgct cacgtcgttc cctcccctat ttgttgccca
      601 tgaggccgcc cggcctccag agcagcacgg cgatcatggc gatgaacgcc agggaatccc
      661 tcggcagcgg gatgttcgcg tagccgatca gcagcgtctc gcccacgccg agcagcagcg
      721 acgcgagcac cgcccccggc agcgagccga gcccgccgac gacgatgagg gcgaggcact
      781 tgtacgccgg aaccgccccc attgtggggt agacctggtt gaagtagatg ccgacgagaa
      841 tgcccgcgac cgccgccacg gcggagccga gggcgaaggt catgctgacg gtgaaggcgc
      901 cgtcgatccc catcgctccc gcgacctccc tgtcctgcga cgtcgcccgc atggcgaggc
      961 cgaactcggt ctttgtcacg atgaaccaga gaagcagcag gacggaggcg ctcacggcgt
     1021 agatggagat gatcgccggg gaaatcacca cgccgctcac ggtgatcccg gaaaagggca
     1081 gctcggcggg gaaggagagg atgtacgggc cgccgacgag gcggaaaatc tcctccagcg
     1141 caaggaaaac ggcgatgctc ccgatgagcg ggacgaacgg ggggaacttc agcagcggcg
     1201 tgtagatcag ccgttcgatg agcacgccga ggagcgcgca gaagatcatc gcgccgagga
     1261 cggccagaag cagactgccg gtgtgcgagt agacgtacag gccggcgtag gccccggcgg
     1321 cgtacacccc tgcgtgcgcc acatggagaa tgcgcaggac gccgtagacg agggccagcc
     1381 cgagcgtgat cagggcgtag atcgccccca tcgccgcccc gttcagaagt tgctcgatga
     1441 agagctgcat gacggatacc aacctccaga aaactccgga ttgcgttccg aaaaagaagc
     1501 ttccgcgcgg agcgcgccgg gaagtttctc tcagcggaac gaaaataatt ctagcacacg
     1561 gggggcggct tcagaaaagc cccggtagtg tatacacgca ctctccgcgt tcgggacgtc
     1621 gaagaaacag ggctttcagc cagttccggc cggcctgctc cgctgcgaaa cgcgtcgcgg
     1681 aatgtcctcg gtgctccgcg cgcagcttca ccccgccgaa cgggtcgaac cccgtcacaa
     1741 gcgctttcat cccgctccat ctcctttcgc aacagcctcg cccgagggga gatcctttcc
     1801 gtacacgatg aaccagatcg tccctgcggc gccgaaggcc gaggccgtca aaaaattgac
     1861 cgccggtccc gccatccaga ggaacgcgcc gccgaaggca gccacggaga ccacggtatc
     1921 ccgcagcagg tagtagaggc cgaacatcgc ggcggactcc ccatgagggg agagatcaag
     1981 gatcagcgat ttcctcgtag gttcgccgaa ctccttcagt ccgcgcaccg cgaaggcgat
     2041 caggagcgcc tcgaaggagc gcgacgcgag gagcgccagc ggaaacgccg tgaagaagca
     2101 gaaggtgatg gtcacgaaga tcttcttccg ccccctgtcg gccagccagg ccacggggat
     2161 gtagatcagc agggcgacga ccatctcgac cgccgtgagc agtccgaact ggaaggccgt
     2221 cacgggagag gcgatcgtct tcatcgccca gacgacggcg aacgcgtagg ggatctgctc
     2281 gcagaagcgg accagaatat cggagacaag cagattcttc agctcggggc tcatcttccg
     2341 gaatatcttc agcgggttcc cctcgggccg ctcgacgaca tcctgatgcg cgtcgtcgat
     2401 gagtcgctgc tggagcagaa gcgcgagcag cgcgaagaaa aacgcgcctc cgaaggcgat
     2461 tctgacgccg agcgactccc cgaacaggcc gatcatcgcg ccgcctacaa tcgggccgag
     2521 cgccatcgga atccgccgta cgagggagtg gacggagact cccatggtgc gcttgttctt
     2581 gggcagcgcc gaggcgatga gcttcatcgt cgccgggagg gagatggccg tccacgagag
     2641 gaacaggaaa gaggccccta tgaccgcttc ccagcggggg acgaaaatca ccacggcaaa
     2701 ccccgtcatc gcaagaatgt tgaagaagag cagcgctcgc ttcgtgccga agcggtcggc
     2761 gatataccct ccggggaagg agtagagcgc cgagaggaga ttgtccagcc cgttcagcat
     2821 gccgatggag agcgcgcctc ccccgagcgt gagaaggtag atcgggagga aacgctccgc
     2881 catccgctct cccaggccca cgaggatcac catggagagc atgccgacga tgcttttttt
     2941 caggccgaag aagtcggcga agcccttccc cggtttcata gttatttgcg tcccgacttt
     3001 cccgatgcca gcagatcgcc cagcgtcacg ccggtggggc gctccgagtc cttgatcgtc
     3061 cggagggccg ccgtgatcgt cgtgcggatc tgctcgggag agtaggaggg ggggaagtag
     3121 agcgccgcgg agtcgtcgtg cccgcccccc cggatcttct tgccgtcgcg gagcatcccg
     3181 accaccttac ccgccgtctc gctccggctg cgcatcgaca cggcccactc gccggagtag
     3241 cgcttcgccg ccaccgcgac aagcagcggc gggttcgcca tccggtcgag caccaggccc
     3301 ccgaagtcgg agacgtcgcc gaactcgagg attccgggct ccacgaaggc aagatcgtct
     3361 ttgctcttgc cgatcttctc caccgcgagg gcgaagcgct tctcctgctt ctcgacgaac
     3421 tccacggcgg ctgcccgctc gtgcggaagg aggacggcct cggggttcgc agccagccgc
     3481 gcgaagaggc tctgcgggcc gcagagggtg atgagcgcct gccagagacg gctgtccgga
     3541 ttctcgttga tccagaggtc ccggtcgttc gccagctcca cgagaggggc gagacgttcc
     3601 attccgggcg cgcccgtccc gacgagccag cggtagtaga ccttcgccgc gcaataattg
     3661 gtgtccacga cggcccacgg gcggttgccg tagcgcgcgg ccgtggtgtc gtgatggtcg
     3721 aagagaaacg gagcgtcgtc ggcaatatac tgtttgtcca gtttttctat tgtcttctcg
     3781 ttctggcaga aaagatcgat gacgacgaac tcctgcccgg cctcgatact ttcgaggatg
     3841 agcgcatcca ccgtgccgta cccggccagc gagaccttca gcggccgcgt cggctgccag
     3901 cgatgccagg cgacggcggc ggcggcgatg ccgtcgaggt cggtgtggct gatgatgtga
     3961 agcagcgagt gatccatatg gtattctcct ttttggcgga acagggtgtc gtcctgcgag
     4021 agtgttgaga aaatgactcc gcactgtatt ctaccctttc cgtccgctct ttgaaactgt
     4081 cgggcaactg aatttccgga cggacggcgg cgtctctttt tcccgccccg gttcacctgg
     4141 agaaacagca attcgtagat tcacaggcat ccacaccctt tatccacaac ggtggaaaat
     4201 ttgtggataa agagctcatt ttgcttcctg caagcgcaga ggagtttgtg gacagaccgc
     4261 gaattttgta acaacttctt tacatccgtc cacatccgcc attcgcccta tccacaaaac
     4321 gaaactcatt ttgtggatat gtggataact tttgtggaca acgtatctct ttccaaggcg
     4381 acaatggatt gaaagggcgg ctttttcccg tttcaggcgc gaatgtgcag cttatccaca
     4441 gtctctgtga taatatggtg ccattctcag catgaggtga agtattgtgg acaacgattt
     4501 acagcatctg tgggatcata ttctcgacga agccggttcg caactccccg cgggggcgac
     4561 ggaaacgtgg ctcagaacct gcgctcccac cgagtttacg gacggcgtgc tcgtcctcga
     4621 cgtccccaat gtcttcgtaa aggagcagat atcctcccgg tttctgaagg agcttatcca
     4681 catctttacg gagacgggcg ggggacggtc gatcgaactc agggtgggcg ccgaaacccg
     4741 gaaggaagag cgggaacggg ccgaacgcgc tgtctccgcc gggaatttgg agtcccgcag
     4801 cggtttgaac ccggggtacc tcttcaactc gttcgtcgtg gggaagtcga accggctggc
     4861 gcacgccgca agcctcgccg tggccgaaac gccgggagag gcctacaacc cgctcttcat
     4921 ctgggggggc gtcggtttag ggaagacgca tctcatgcac gcgatcgggc actacgtgct
     4981 cgaccggaac tcgaactcgc gcgtgacgta cgtcagctcg gagaagttca tcaacgagtt
     5041 catccagtcg atcaagaaca acaagacgca ggagttcaag tcgaagtacc ggagcgtgga
     5101 cattctgctc atcgacgaca tccagttcct cgggaacaag ggaagcagcc aggaggagtt
     5161 tttccacacg ttcaaccagc tccacacatc gaagaagcag atcatcatct gctccgaccg
     5221 gcctccgaaa gagatccaga gcatcgagga ccgtcttgtg agccgtttcg aatgggggct
     5281 ggtgaccgac atccagtcgc ccgacctgga gacgcgcatc gcgatcctcc agaagaaggc
     5341 gcagctccgc cgctacaaca tcccggacga cgtgatcaac ttcctcgcga tcaacatccc
     5401 gagcaacatc cgcgagctcg agggcgcgct caaccgcgtc gtggcctgcg cagaactcgc
     5461 cgccgagccc atcaacgagg agaacacgcg ggagtggctg aaggacgtca tcagaaaaga
     5521 cctgcgcggc cctgtgagca tcgactccat tcagaatacc gtcgccgatt tcttcagcat
     5581 gtcggtggac gaactgctca gctcgaagag gacgtccgat ctcgcgatgg cccgccagat
     5641 cgcgatgtac ctctgccgga agatcacgga gtcgagcctg cagcagatag gcatggcgtt
     5701 caagaagaag gaccacacga cggtgctcca cgcgcacaga aagatagcgc agatgatcaa
     5761 agagcagccg cggatcaaac agattgtgga tacaatagag ggcaagctgt gaaactcggg
     5821 gtcgatatgt ggataactct cgcagaatga agaaatgtac ggaatgtcct gtggacaatg
     5881 tggataattt ctgaatgaat cgcccaggtc tgtccacagt gaagtcgttc gtcttccaaa
     5941 ggtccgggga cggttatcca caattcgcac accccttatt accattactg ctactaaaag
     6001 ataaagaaca ataaaagatg agacccggga gggaaaggca tgagactgca aatcagcaag
     6061 ggagaattca tgaaaaactg gcagatcgcc gaaaggagca caagctctaa aagtacgatc
     6121 aattccctca caggagtttt actgcggact gccgacgcgg acaacagcgt acttatggag
     6181 gctacggatc tgaagacctc gatcaagtgc atctcgcagg gcgttctcgt cgaagaggag
     6241 ggagaagccg ttcttccggt caaacttgtc ggcgagctct tcaagaaagc gccgacgaac
     6301 gtcttcaccg tctccatcac cgacggcaag gggatcatca tcgcggggcg gaacaagtac
     6361 aagttcacca cgtactctcc ccgtgagttt cccaagcttc cggtctccga aagcgcggcc
     6421 tttttctgta cggcgacggc gtcggagctg cttcgcgcgg tctccgaggg aaccgttgcc
     6481 tgcactacgg gcgaggagtt tccgaagtac cttggaacgg cttttttcca gcttcgagac
     6541 ggagaaatgc gcgtcgtcgc cacggatggc agacgtctct ccctgtcaaa gtgttccccc
     6601 gtacgcaccg gagaaaactg cgactttctc cttcccatca cgggagtccg tgagctgcaa
     6661 cgcctccttg gcgcgcttga cggcgagtct cccatcgaga tccttgttga aagcacgctc
     6721 gtgttctttc agatgggctc tatggagttc tccgtccgca gagtggatac cgcattcccg
     6781 aattatgaaa acatactgaa tccgcagacg acaacgacga tggatatcga caggcacgaa
     6841 ctgatcgccg cgctcgaacg cgtggacgtc atcgtgcgcg atttcagtca catggtgctc
     6901 gtccgtcttt cgccggaagg cgatctcctc cttacgggga aggcgcccga gaccggagcc
     6961 gttcaggagg ttctcgacgc gaagatcgag ggagaatcgc tgaccgtggc cttcaacgtc
     7021 tctttcctga tcgaagggct gaaagccctg tatggcgacc gcgtcttcat cagcttcaac
     7081 ggcccggacg gacagatgac catgctcaga ccgggggaga aggacttcct ctacatggcg
     7141 atgccgatca agattaacga gagcgattta ctccacggcg aaatggacga ggaaaacgag
     7201 acggagattc ccgaataggg gagctgaatg cgctgcgggg cgcttcgtct tcgaaacttc
     7261 aagaatataa cgacggagac gattctgtgg ggcgaaggat tgaatctgct cgtcgggaag
     7321 aacggcgccg ggaagacgaa ctgtcttgag gcgcttcatc tgctgctcgg ctggggaccg
     7381 ctgggcgaca ggaaagatat tgcttcctgg ggcagcgccg agaaaaagac gtacgtcacc
     7441 ggcgacttct tcggcgagga ggaggcgttt ctcgccgtcg gcatcggagg aagcaccgtc
     7501 gtcaagtaca atgggaaacg ctgctccttc cccgaagtcc gttccgtcgc tccgtcgctc
     7561 gcgtttcttc cttccgacat ggagctgctc gacggatctc ccgcccggag gcgttcgttt
     7621 ctcgaccgtc tgtgcgcgct gctctggccg ctctatgcgc ggcgtctcgt cgagttccgg
     7681 cgggcggtgc gccacagaat cgtgctgctc cgccagggag gaaacctcgt cgcgatctcc
     7741 aaggcgatgg cgccgctggc ctcgtggctc tggggggcga gagcgtccgc cgtttcggcg
     7801 cttgccgagg gacttgcggc gcttcctgat cttctgccgc tgccggttcg gctggcgcac
     7861 gtcagaggcg gcgcggggag agtgaaggac cctcttgagg actggtggga gtccctcgaa
     7921 gcgcggaggg agaaggagcg tctctgctg
//
LOCUS       DAOPKU010000066         7898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9756_length_7898_cov_4.648094, whole genome shotgun sequence.
ACCESSION   DAOPKU010000066 DAOPKU010000000
VERSION     DAOPKU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9756_length_7898_cov_4.648094"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PK849_03255"
     CDS             complement(<1..356)
                     /locus_tag="PK849_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HPE65173.1"
                     /translation="MRTLSAETSYSRRLDALFKHPAADAAMLVLISMSVAVAAGSLLV
                     DNEELEHLNLYFSLLFSVELTARYFTYASNKREYFADWWMDWIAAIPWDIFLFFLFPG
                     GGASMLRLLRLPRIFRL"
     gene            complement(402..1880)
                     /locus_tag="PK849_03260"
     CDS             complement(402..1880)
                     /locus_tag="PK849_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE65174.1"
                     /translation="MRTVRRKSLFLSVFILTAFILSHVPSEAAVRPLKQSYAPDEPIE
                     IVYDNLPGSQTDWISLAPRENADDRYGEWFYTGGMRSGSHTFGGLPAGVYEARVYHDW
                     PAGGYVVQERAAVFVQEAVPEQSGPVPAEIPAAMRPTPRPEPHPSPVPEPVEISWSAS
                     GQGTLFAPGQGVHNYRIHELDARRFLNGGTLIVDVRIGAGQSWASVDLYGDGQSLATS
                     GVPQALASAWDLPPNSNRRITHTFRKGQIFRLCFEGNWGSPQGATNTYTYSATIKPAP
                     EHEPQLPPLPERETPHVAPPENAVNRRAAETLWRQAVSFQQQKRYEEALAKYREGLAL
                     SEDPAVAHHADAMETCLLRLSTLPDIAATRPEAAEIDIFNNWNKTAVMNNPAGPPSPT
                     RFTISRPHAITYINTYHYFNNGTPAGSITLVHQDGTLYGPWPATGRSGQGGVQNAIWE
                     ATPQTTLKAGTYTVFDSHPSTWSHNAQSDLRGFALVKGRLLQ"
     gene            complement(2082..2561)
                     /locus_tag="PK849_03265"
     CDS             complement(2082..2561)
                     /locus_tag="PK849_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HPE65175.1"
                     /translation="MFWFGKKNLKLLAPLSGRVVPLEEVPDKVFAGKMLGDGVAIDPD
                     DDGLVVAPCDGDLVALFPTGHAFGIRGHKGIEVLVHIGIETVALEGEGFTLIAKAGSK
                     VKAGDPIVRFDKAIVAAKAPSILTPVIITTGDRVKSFKAASGTVRAGKDVLLDIEPA"
     gene            complement(2567..3556)
                     /locus_tag="PK849_03270"
     CDS             complement(2567..3556)
                     /locus_tag="PK849_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65176.1"
                     /translation="MVRRILDANASDLAKMTGRELLTSIRAAEGRTLVAETIAPAPPL
                     LGDVTNAELARAFGADIILLNMFDVRAPHIEGLDVLNDDDFFLRTYGQGHRVRATETT
                     RAQTGTIAALKTLIGRPIAINLEPVDEGERVLGPQAKLPEGRRATPENARLAVEQGVD
                     MLVVTGNPATGVTTSSITASLRGIREAVGPDPILAAGKMHAAGSATDAGSDIVDEAII
                     GRWIDAGADIVLLPAPGTVPGISPERVRDLIGVVHAKGALAMTAIGTSQEGADEATIR
                     QIALWAKSCGADLHHIGDSGYPGIAVPENILAYSIVIRGRRHAWRRMAASIAR"
     gene            complement(3594..4331)
                     /gene="nagB"
                     /locus_tag="PK849_03275"
     CDS             complement(3594..4331)
                     /gene="nagB"
                     /locus_tag="PK849_03275"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPE65177.1"
                     /translation="MRIVTAKDYDQMSRKASYIVASRVILNPKCVLGLATGDTPKGLY
                     NELVRLFRAGDVDFSGVTTFNLDEYAGLSPENPQSYHSYMEEHLFRHINIPSEHRHIP
                     NGAAQNIGAECRRYEELIEQAHGIDLQILGLGRDGHIGFNEPDVKFEKGTHHVRLTQS
                     TIEANARFFENRDEVPKSAISMGIKTIMSARRILLLASGPEKAEAIRGAVMGAVTPDL
                     PASVLQLHPNATLIVDEAASAEIMRIK"
     gene            complement(4303..5460)
                     /gene="nagA"
                     /locus_tag="PK849_03280"
     CDS             complement(4303..5460)
                     /gene="nagA"
                     /locus_tag="PK849_03280"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPE65178.1"
                     /translation="MKAITGGKIVAGRTWLGGMVLLFDGRVLDILPEGELPAGVETLD
                     AEGAWIVPGFVDIHVHGVCGVDTMDGAAESLSAIAGGLAAHGVTAFCPTTMTMDEARI
                     EAALDTVKAAKNAPPDGARVLGAHLEGPYISPSRLGAQDGTHLRRPDPEFVRRRKSAL
                     RLVTFAPEEDTDGRFLEALREEGIVASLGHSQASYEQAMKAFRNGAKSVTHLFNGMPP
                     FHHRTPGLAGAALDADVFCELIADGVHSHTAVFRLLLKMKGVERIVLVSDAMRGACLG
                     EGEWDLGGQTVTVHGPEAKLADGTLAGSVLTLDLALRNFAAATGLPLWEATRLVSENP
                     ARLLGERDRGRIEPGYRADFVLLDEGWRVLKTFVEGKEVFDRHENRDCKGL"
     gene            complement(5619..7001)
                     /gene="nagE"
                     /locus_tag="PK849_03285"
     CDS             complement(5619..7001)
                     /gene="nagE"
                     /locus_tag="PK849_03285"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HPE65179.1"
                     /translation="MLKGLQKLGKALMLPVAVLPAAALLLRLGASDVLDIPFIMQAGA
                     AIFDNLALLFAVGIAVGVAFDGGGAAALAGAVGYFTLTKAVVTINSTINMGVLAGIVS
                     GIVAGMLYNKYHNIKLPDFLGFFGGKRFVPIAASLASIVLALVFGYIWPPIQQAIHGV
                     GEWLLGAGLLGAFTFGTLNRLLLPLGLHHVLNSMVWFVFGEFTNAAGQVVTGDLHRFF
                     AGDPTAGVFMTGFFPIMMFALPAAALAMYTTARSANKKAVSGILLSVALTSFLTGITE
                     PIEFAFMFLAPVLYIAHSLLTGAALALCSMLGVLHGFGFSGGAIDYVLNYGLSTKGWM
                     IIPIGLAFAAVYYFLFVAVIKGMNLLTPGREEEATGATASAVSTADLENLAKEYIARL
                     GGAQNIEEIGACITRLRLVLKDGKVVEEAQIKALGATGLIRPNATTLQVVVGTKAELL
                     ADVMKQHMKK"
     gene            complement(7050..7784)
                     /locus_tag="PK849_03290"
     CDS             complement(7050..7784)
                     /locus_tag="PK849_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE65180.1"
                     /translation="MRVLDKESPVPLYYQLKEILQAMVDDGDLAPGDSVPSERDICER
                     FSISRMTASKAVSALVDEGVFHRERGRGTFVSYPKPTCTSSSLTGFSENIRAAGLDAM
                     THILAFDEEEASRTIRESLQLPQHDPIVFNILRLRYVEGEPFSLENAWVPKCRFPELS
                     RDLLEGRSLYTLMREHFGMKLSHASQTIEPVFCSDFEAKNLDLDSGTPVLLFRRVAYA
                     EPQTPVEYSKCVYRGKRFKYEISFGI"
BASE COUNT         1380 a         2435 c         2603 g         1480 t
ORIGIN      
        1 agccggaaga tcctcgggag ccggagcagt cggagcatcg aggcgcctcc ccccgggaag
       61 aggaagaaga ggaatatatc ccacggaatg gcggcgatcc agtccatcca ccagtcggcg
      121 aagtactctc gcttgttcga cgcgtaggtg aagtagcgcg ctgtcagttc caccgagaag
      181 aggagcgaga aatagaggtt gaggtgttcg agctcttcgt tgtcgacaag cagcgatcct
      241 gccgcgacag cgacggacat cgatataaga acgagcatcg ccgcgtccgc cgcggggtgc
      301 ttgaagagcg cgtccagccg ccgtgaatag cttgtttctg cggaaagagt ccgcatgaca
      361 tctcctcctt ctcataccac cggagcatac accggtagaa tctactgaag caggcggcct
      421 tttacgagcg cgaagccgcg cagatcggac tgcgcgttgt gtgaccacgt cgacgggtgc
      481 gagtcgaaga cggtgtacgt cccggctttc agcgtcgtct gcggggtcgc ctcccatatc
      541 gcgttctgta cgcctccctg cccggagcgg cccgtcgcgg gccacggtcc gtagagcgtg
      601 ccgtcctggt ggacgagcgt gatgcttccg gccggagttc cgttgttgaa gtagtggtac
      661 gtattgatgt aggtgatcgc gtgcggccgg gagatcgtga agcgcgtcgg cgagggcggc
      721 ccggcggggt tgttcatgac ggcggttttg ttccagttgt tgaagatatc aatttctgcc
      781 gcttccggac gggttgcggc gatgtccgga agagtgctca gccgcaggag gcatgtctcc
      841 atcgcgtcgg catggtgtgc gaccgccggg tcctcggaga gagcgagccc ttcccggtat
      901 ttcgccagcg cttcttcgta ccgtttctgt tgctggaagg agacggcctg ccgccagagc
      961 gtctccgcag cgcgccggtt taccgcgttt tctggcggag cgacgtgcgg tgtttcacgc
     1021 tcggggaggg gaggaagttg cggctcgtgc tcgggagcgg gcttgatggt cgcgctgtac
     1081 gtataggtgt tggtggcgcc ctgcggcgat ccccagttcc cctcgaagca gagacgaaag
     1141 atttgccctt tccggaaagt atgcgttatg cgccggttcg agttcggagg cagatcccat
     1201 gcgctcgcaa gggcctgcgg cactccagag gtcgcgaggg actgaccgtc cccgtacagg
     1261 tcgaccgacg cccagctctg ccccgcgccg atgcggacgt cgacgatcag cgtaccgccg
     1321 ttgaggaagc gcctggcgtc cagttcgtgg atgcgatagt tatggacgcc ctgtccggga
     1381 gcgaagagcg ttccctgtcc cgacgcggac catgagatct cgacgggttc ggggacgggg
     1441 gagggatggg gctccgggcg aggagtcgga cgcattgccg ctggaatctc ggccggaacc
     1501 ggaccggact gctccggaac ggcttcctga acgaagacgg cggcgcgttc ctgtacaacg
     1561 tacccgcccg ccggccagtc atggtagact ctcgcctcgt agactcctgc gggcaatccg
     1621 ccgaaagtat ggctcccgga gcgcatgccg ccggtgtaga accactcgcc gtaccggtcg
     1681 tcggcattct cccggggagc aagcgaaatc cagtcggtct ggcttccggg aaggttgtcg
     1741 tagacgatct caatgggttc gtccggcgcg taggactgct tgagcgggcg gacggcggcc
     1801 tcggaaggca cgtgtgacag aatgaatgct gtcagtatga acacggagag aaaaagagac
     1861 tttcgtcgta cggtacgcat agagtgtact ccccctcgct cgtgtgaata gtgaccccat
     1921 gataccagaa gcagggcttt tttcgcaaaa gaggagcgtt ttcatttttg gagaaaggtc
     1981 cgcggacgtg ggcgggcgtc ggaacgctct tcgagcagtc ttcagcgaac ggagagaagg
     2041 ggatgcggtg tggttccccc tctttccgtc cttttcagcg cctacgccgg ttctatgtcc
     2101 aggaggacgt cttttcccgc gcggaccgtg ccggaggcgg ccttgaagga ttttacacgg
     2161 tcgcccgtgg tgatgatgac cggcgtgagg atggaaggag ccttcgctgc gacgattgcc
     2221 ttgtcgaagc ggacgatcgg gtcgcccgcc ttcactttgc tccccgcttt tgcgatcagc
     2281 gtgaaccctt ctccctcgag cgccactgtt tcgatgccga tgtgcacgag gacttctatt
     2341 cccttgtgtc ccctgatgcc gaaggcgtgc ccggtcggaa agagcgcgac cagatccccg
     2401 tcgcatgggg cgacgaccag cccgtcgtcg tcagggtcga tagccacgcc gtcgccgagc
     2461 atcttcccgg cgaagacctt gtcggggacc tcctccagcg gaaccacccg tccggagagg
     2521 ggagccagca gtttcaaatt tttcttgccg aaccagaaca tgcgcgtcac ctcgctatgg
     2581 atgccgccat ccggcgccat gcgtgacggc ggccccggat gacgatggag tatgccaaaa
     2641 tgttttccgg cacggcgatg cccgggtagc ccgagtcgcc tatgtggtgc agatccgcgc
     2701 cgcaactctt ggcccagagc gcgatctgcc ggatggtcgc ctcgtccgcc ccctcctgcg
     2761 aggtgccgat tgcggtcatt gccagcgccc ccttggcgtg gacgacgccg atgaggtcgc
     2821 gcacccgctc gggcgagatt cccggaacgg tgccgggggc ggggaggagc acgatgtcgg
     2881 cgcccgcgtc gatccaccgg ccgatgatcg cctcgtcgac gatgtcgctt cccgcgtcgg
     2941 tagcggagcc tgccgcgtgc attttccccg cggcgaggat cgggtcgggc ccgacggcct
     3001 cgcggattcc ccggagcgac gcggtgatgg agctggtggt gacgcccgta gcggggttcc
     3061 ccgtgacgac gagcatgtca accccctgtt cgacggcgag acgggcgttt tccggcgtgg
     3121 cgcgccgccc ctcggggagc ttcgcctgcg ggccgagcac gcgctctcct tcgtcgaccg
     3181 gttcgaggtt gatcgctatg gggcggccga taagtgtttt gagcgccgcg atggttccgg
     3241 tctgagcacg ggtcgtttcg gtcgcccgca ccctatgtcc ctggccgtac gtgcgcagga
     3301 aaaaatcatc gtcgttcagt acgtccagcc cttcgatgtg cggagcacgg acgtcgaaca
     3361 tattcagcag gatgatgtcc gcgccgaaag cacgtgccag ctcggcgttc gttacgtcgc
     3421 caagcagcgg gggggcgggg gcgatggtct cggcgacgag cgtacgtccc tccgccgcgc
     3481 ggatgctcgt cagcagctcc cgccccgtca tcttcgcgag gtcggaggcg ttggcgtcca
     3541 gaattcgtcg taccatgatg atgctccttt cctgagtaca gcagttctgc cggtcacttg
     3601 atgcgcatga tttcggcgct tgccgcttcg tccacgatga gcgtcgcgtt ggggtgcagc
     3661 tggagcaccg acgcgggcag gtcgggggtc accgccccca tgaccgcgcc gcggatggcc
     3721 tccgccttct ccggtcccga ggcgagcagc aaaatccgcc gcgcgctcat gatcgtcttg
     3781 atccccatgc tgatggcgct cttgggcacc tcgtcgcggt tctcgaagaa acgggcgttc
     3841 gcctctatgg tgctctgcgt gagacggacg tggtgcgtcc ccttctcgaa cttcacgtcc
     3901 ggctcgttga agccgatatg cccgtcccga ccgagtccca ggatctggag gtcgatgccg
     3961 tgcgcctgtt cgatgagttc ctcgtagcgg cggcactccg cgccgatgtt ctgcgccgcg
     4021 ccgttgggaa tatgccggtg ctccgacggg atattgatgt gccggaaaag atgttcctcc
     4081 atgtagctgt ggtagctctg cgggttctcc ggcgagagcc ccgcgtattc gtccaggttg
     4141 aacgtggtga cgccggagaa gtcgacgtcc cctgcccgga agaggcggac cagttcgtta
     4201 tagagcccct tgggggtgtc ccccgtggcg agcccgagca cgcacttcgg gttcaggatg
     4261 acacggctgg cgacaatgta cgacgccttc cggctcatct ggtcataatc ctttgcagtc
     4321 acgattctca tggcggtcga agacctcctt tccttccacg aatgtcttga ggacgcgcca
     4381 cccttcgtcg aggaggacga agtcggcccg gtaccccggc tcgatgcgcc ccctgtcccg
     4441 ctcgcccagc aatcgggcgg ggttctccga gacgaggcgc gtcgcctccc agagggggag
     4501 gccggttgcg gccgcaaagt tccgcagcgc gagatcgagc gtgaggacgc ttccggcgag
     4561 cgttccgtcg gcaagcttcg cctccggacc gtggaccgtg accgtctggc ctcccaggtc
     4621 ccactcgccc tcgccgaggc aggcgccgcg catggcgtcg gagacgagaa cgatgcgctc
     4681 gacgcccttc atcttcagca gcagccggaa gacggccgtg tgcgagtgaa cgccgtcggc
     4741 gatcagctcg cagaagacgt ccgcgtcgag cgcggctccc gcgagaccgg gggttctgtg
     4801 gtggaatggc ggcatgccgt tgaaaaggtg cgtcacgctc ttcgccccgt tgcggaacgc
     4861 cttcatcgcc tgctcgtaac tcgcctgcga atggccgagc gaagcgacga tcccctcttc
     4921 ccggagcgct tccaggaaac gaccgtccgt atcttcttcg ggagcgaacg tgacaaggcg
     4981 cagggcgctc tttctgcggc gcacgaactc cgggtcggga cggcgcaggt gcgttccgtc
     5041 ctgcgcgccg aggcgggacg ggctgatgta ggggccttcg aggtgcgcgc ccagcaccct
     5101 cgctccgtcc ggaggcgcat ttttcgccgc tttcactgtg tcgagcgccg cctcgatgcg
     5161 cgcttcgtcc atcgtcatgg tggtcgggca gaaggcagtg acgccgtgtg ctgccagccc
     5221 tcccgcgatg gccgagagcg attccgccgc cccgtccatc gtgtccacgc cgcagacgcc
     5281 gtgcacgtgg atgtccacga atccggggac gatccacgcc ccctccgcgt cgagcgtttc
     5341 gacacccgca ggaagctcgc cctcagggag gatatcgaga accctcccgt cgaagagaag
     5401 aaccataccg ccgagccaag tgcgccccgc gactatttta ccgcctgtga tcgctttcat
     5461 tgccgtacct ccgttgttgc cgattacgaa cgccctcccg tacagtcggc gaagggaggg
     5521 cgtctacaaa aaatccggga aatgccgcgt ccgcggcgca aagccgcgag agtgtccttc
     5581 cgcggggcgt ctcgggatgg aacgccccgc ggaagctttt attttttcat gtgctgtttc
     5641 atgacgtcgg ccagcagctc ggccttcgtt ccgacgacga cctggagcgt cgtcgcgttg
     5701 ggacggatga gtcctgtcgc gccgagggct ttgatctgcg cttcctcgac caccttgccg
     5761 tccttcagga cgaggcggag acgggtgatg cacgcgccga tctcctcgat gttctgtgcg
     5821 ccgccaaggc gggcgatgta ctccttcgcc aggttttcaa gatccgccgt ggagaccgcg
     5881 gaggctgtgg cgccggtcgc ctcttcctcg cgtcccggag tgagcaggtt catccccttg
     5941 atgaccgcga cgaagaggaa gtagtagacc gccgcaaagg caagcccaat ggggatgatc
     6001 atccacccct tcgtggagag tccgtagttc agcacatagt cgatcgcgcc ccccgagaag
     6061 ccgaatccgt ggagaacgcc gagcatggag cagagcgcca gcgcggcgcc ggtcagcaga
     6121 gagtgcgcga tgtagaggac gggcgcgagg aacatgaagg cgaactcgat cggttcggtg
     6181 ataccggtca ggaaggatgt cagcgccacg ctgagcagga ttcccgaaac cgctttcttg
     6241 ttggccgaac gggccgtggt gtacatcgcc agagcagccg caggaagagc gaacatcatg
     6301 atggggaaga agcccgtcat gaagacgccc gccgtcggat cgcccgcgaa gaagcggtga
     6361 aggtcgcccg tgaccacctg tccggccgcg ttggtgaact cgccgaagac gaaccagacc
     6421 atgctgttga gcacgtggtg caatccgagc ggaagaagca gccggttcag cgtgccgaag
     6481 gtgaacgcgc cgagcagtcc cgccccaaga agccactcgc ccacgccgtg gatggcctgt
     6541 tgaatcgggg gccaaatgta gccgaagacg agcgcaagga cgatgctggc aagggatgcc
     6601 gcgatcggaa cgaagcgctt gccgccgaag aagccgagga agtcggggag cttgatatta
     6661 tggtacttat tgtagagcat tcccgctacg atgccggaga cgatgcccgc gagcacgccc
     6721 atgttgatcg tcgagttgat ggtgacgacg gccttggtca gcgtgaagta gcccaccgcg
     6781 ccggcgaggg ccgcggcgcc gcctccgtcg aacgccacgc ccaccgcgat gccgacggcg
     6841 aagagcagcg ccaggttgtc gaagatcgcc gcgcccgcct gcatgatgaa cgggatgtcc
     6901 agcacgtccg acgcgccaag cctgagaagc agcgccgccg ccgggagcac cgccaccgga
     6961 agcatcaacg ccttaccgag tttctgcaaa cctttaagca tgtacaacac cctcctctat
     7021 tgaattgggg ggccttgccc cctaaagccc taaataccga aggaaatctc gtacttgaat
     7081 cgttttcccc tgtagacgca ctttgagtac tccaccggag tttgcggctc ggcgtaggcg
     7141 actcttctga agagcagcac cggagttccg gagtcaagat cgaggttctt ggcctcgaag
     7201 tcggagcaga ataccggctc gatggtctgc gaggcgtgcg aaagcttcat gccgaagtgc
     7261 tcccgcatca gcgtatagag cgaccgcccc tccagcagat cgcgcgagag ttccggaaaa
     7321 cggcacttgg gaacccatgc gttctcgagc gaaaaaggct ccccctccac gtagcggagg
     7381 cgcaggatgt tgaagacgat ggggtcgtgt tggggaagct gcaaggactc gcggatcgtg
     7441 cgggacgcct cctcctcgtc gaaggcgaga atgtgcgtca tcgcgtcgag tccggcggcg
     7501 cggatgttct cggaaaaacc ggtgagactg gaggatgtgc aggtcggctt gggataggag
     7561 acgaacgtac cccttccccg ttctcggtga aagacaccct cgtccaccag cgcggagacg
     7621 gccttgctcg ccgtcatgcg gctgatggag aaccgctcgc agatgtcgcg ctcggacggg
     7681 acgctgtcgc ccggcgcaag gtcgccgtcg tcgaccatcg cctgcaaaat ctctttgagc
     7741 tggtagtaga gcggaaccgg gctctctttg tccagtacgc gcatgggcaa tttccccccg
     7801 agaggtagag ttgtctgaca aagagagccc ggttccgctc tactaccagc tcaaggagat
     7861 tctgcaggcg atggtcgacg acggggagct tgcccccg
//
LOCUS       DAOPKU010000067         7823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9916_length_7823_cov_5.132853, whole genome shotgun sequence.
ACCESSION   DAOPKU010000067 DAOPKU010000000
VERSION     DAOPKU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9916_length_7823_cov_5.132853"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK849_03295"
     CDS             <1..382
                     /locus_tag="PK849_03295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPE65181.1"
                     /translation="PHGSIICGMSPQGHPEDFENKTTWQFIEAMSKTLVKLLPRTKGI
                     RIARQWSGLYDMTPDAQPVIGETDVKNFYHSTGYSGHGFMLAPVAGKILAQHITGQKP
                     DIDFSMLDYRRFSRGEHIRESNVV"
     gene            418..813
                     /locus_tag="PK849_03300"
     CDS             418..813
                     /locus_tag="PK849_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE65182.1"
                     /translation="MKFLKPLLDKCVMCGACMSACSKAWAKQDNPAMSRIAVKNVTGL
                     PDIRVCDQCGGCIAVCPTMALQRDKNGVVQIDRKKCTSCLICVEFCPSASMFFHADID
                     SPFKCVACGICVKACPEGALSIVEQKQEG"
     gene            818..>1896
                     /locus_tag="PK849_03305"
     CDS             818..>1896
                     /locus_tag="PK849_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPE65183.1"
                     /translation="MEQMKLLTEWEYTPAKLNKGYTRQFLSVDLGKADGAFTCETHPI
                     SDEMVEKFTGGRGFGLRILWDKVTSKSKWNDPENAMVISGGPLSGTTQYPGMGKFYAV
                     FLSPLTQQTYNSNAGGYFGPFLKAAGFDALSVSGKAQKDVVVYIDGDEGAVRIFEWAP
                     ENRNSYTVTEALHEFFAENEEDKRNISVVSAGEAAEHSWWGAMNVSFYDPRRALPRLK
                     QAGRGGGGTVLRDKKILALVVKKKGFSGTENAPADLSTLQKVGTKLHREIRQNDDVQC
                     LMRKVGTAHLNLVMNDYDILPVNNFKFGRNEDIHNIAPDAYEKLFTQGYADGCWYGCS
                     MACAKAVDGFELTTGPDKGKKVTVDG"
     assembly_gap    1897..1996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1997..2806
                     /locus_tag="PK849_03310"
     CDS             <1997..2806
                     /locus_tag="PK849_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPE65184.1"
                     /translation="VGKGVRKMKEIFSEKYGADRTVMERIGMEGQGLEVSQYRCQESV
                     AQWGGYFLTLKGPQHDEAWVIFMDMVNKQLPTFEDKAEALFYFPNFRLWFSLTGVCKL
                     PWNDVEPSDNRIRYKGIEAAKVPEHVQNYLDVYTAVTGKPLSKEEMILQSERVYNFER
                     VFNLRMGKGTRADHNIPDRALGPVFEDEWNARPEYFDGKLSEAGISPEGLPLKEKMTK
                     LLEFRRSEWEKLVDAVYKRRGWNANGVPTLETLKRLGIDFPEVVDVVKAHQ"
     gene            2834..3031
                     /gene="thiS"
                     /locus_tag="PK849_03315"
     CDS             2834..3031
                     /gene="thiS"
                     /locus_tag="PK849_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPE65185.1"
                     /translation="MIRVNGREMEWRRGMTIQDVLDECGYTFRMIAVWIDGTPYRREH
                     FATAVVPDRAEVQALHMISGG"
     gene            3040..3975
                     /locus_tag="PK849_03320"
     CDS             3040..3975
                     /locus_tag="PK849_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPE65186.1"
                     /translation="MLFGKEPTGFIFDVKRYSIHDGPGIRTTFFFKGCPLSCWWCHNP
                     EGISPLPAPTYHGNRCIACGRCVRECPNGARSFGPEGIALDSSKCVECRICEQVCPTR
                     ALEFVGKEMTVEEIVRLAKKDLAFYDESGGGVTFSGGEPLMQAEFLIACLEALRSLEI
                     STAVDTSGFCREEDLLLAAEEADIFLFDIKLIDPKLHEYYTGVGNERILANLRSLDKK
                     LVEQGRGSINIRIPLIPGVNNSPENLEAVIACVLSLRSVSGVNILPYHSIGSGKYKNL
                     GLEFKMGQTQPPSDYEVSQALSLFASRGIHAVKGG"
     gene            3981..6341
                     /locus_tag="PK849_03325"
     CDS             3981..6341
                     /locus_tag="PK849_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HPE65187.1"
                     /translation="MNERIARLRNESFETYPSISIERARLTTDFYRANEGKYPVPVMR
                     ALNFKNLCEQKTLYIGKDELIVGERGPAPKAVSTFPELNCHTARDLEILNTRPMTGYR
                     VHEDDIRIYTEEVVPYWTGRTLRDRIFSNLPEEWLALYEAGCFTEFMEQRAPGHTALD
                     GTIYSTGLLDMKKKIASARERLDWINDPHATEKDEELQAMDIACDAAIIFARRHADLA
                     EEMAASEKDFVRKAELLKIADVCRHVPANAPRDFHEAIQMYWFTHLGTITELNGWDAM
                     SPGHFDLHLRPFYEKGIADGSLDREKAKELLSCFWIKVNNTPAPPKVGVTAAESGTYN
                     DFTQINLGGIAEDGSDGSNDVSYIILDVLDELQLLQPQVSVHISSKTSERFLRHACKV
                     MRRGSGYPSAFNSEAVFMEQLGMGKTVEDARTGGTSGCVETGSFGKEAYILHGYLNVP
                     KIMELALNNGTDPLSGKRIGLETGAPQTFTCYEEFYQAFEKQLHYVVDTKIRTDNYIQ
                     RMFARHAPAPYLSVLIHDCIENGRDYYDGGPRYNTDYIQCCGIGTVTDSFSVLKKHVF
                     EEKTVTLADLLRALHENWEGWEEMRQFIWNRTPFFGNDDEEADSIMQKVYASLLNAID
                     GKHSPKGPTYHVNMLSTTCHIYFGKKLGATPNGRFAHAPESDGTSPSHGADRNGPTAV
                     VKSLSRMDQLKSGGTLLNQRFLPSVLSGDSGLEKMGQLIRTYFKLGGHHIQFNVVDTE
                     TLRKAQKNPDEYRSLLVRVAGYSDYFVDLDQYHQEEIIARTAQENL"
     gene            6473..7489
                     /gene="dctP"
                     /locus_tag="PK849_03330"
     CDS             6473..7489
                     /gene="dctP"
                     /locus_tag="PK849_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE65188.1"
                     /translation="MQKAKKFVTLCILSALVLFLIVPNMASAAPEMTLRFAGQVPLEH
                     TATKLMHQVADEVKEKTNGRIVVEVYPANQLGDYTLVYEELIRGTIDMAAISVPSQFD
                     PRLEVTYINCFVRNFDEAKSVFAPDGWLFKKMDELSARLGVKLLGFQVEGFIGIGSTK
                     PVNEPLNPNVDKGVLVRIPNMDVYKLGAEAMGYRTVTVPWAEVYTALQTGVADGVDGM
                     TPTAAYTMLKDVLKYWYDLNYSVENFNYMISMKTWEKLSEADRKVIADACAKVTEMSI
                     ELAKQDQEKHLQLMRDAGIEVHTYTAEELKPIFEKVSATWGQLSDKFTPELIEEFKKE
                     YAPK"
     gene            7567..>7823
                     /locus_tag="PK849_03335"
     CDS             7567..>7823
                     /locus_tag="PK849_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65189.1"
                     /translation="MADNETIGCFPENRPEKQSKVDLILLFFFSIVSVSCSLLLSIII
                     SAAAFTRYIIGGDLYGFEEWVRLLAFWLYFMGGAYGAYNDT"
BASE COUNT         1989 a         2018 c         2054 g         1662 t
ORIGIN      
        1 tccgcatggc tccatcatct gcggtatgag tccccaaggg caccccgagg atttcgagaa
       61 caaaacgaca tggcagttca tcgaagcaat gtcgaaaacg ctcgtcaagc tccttccgag
      121 aacaaaaggc ataaggatag cgcggcagtg gtcaggactg tacgatatga ctccagatgc
      181 gcagccggtg atcggagaga ccgacgtcaa gaatttctat cactctacgg gatattccgg
      241 acacggattc atgctcgccc ccgtagccgg gaaaattctc gcccagcata tcacaggaca
      301 aaaaccggac atcgattttt cgatgctcga ttatcgtcgc ttcagccgcg gagagcacat
      361 cagagaatca aacgtggtat gatggaaaga tagatcacca gaaaaggagt gtttgttgtg
      421 aagtttctga aacctttact cgacaagtgc gtcatgtgcg gagcatgtat gtctgcatgc
      481 tcgaaggcgt gggccaagca ggacaatccc gcgatgtcgc gcatcgccgt caagaacgtg
      541 acagggctgc ccgacatcag ggtatgcgat cagtgcggcg gctgcatcgc cgtctgtcca
      601 acaatggcgc tgcagaggga caagaatgga gtcgtccaga tagatcggaa aaagtgcaca
      661 tcctgcctca tttgcgtcga gttttgtccc tccgcctcca tgttcttcca tgcggacata
      721 gactctccgt tcaagtgcgt cgcctgtgga atttgtgtaa aggcgtgccc cgagggcgct
      781 ctgtctatag tcgagcaaaa acaggaggga taggagaatg gaacagatga agcttctcac
      841 cgagtgggaa tacactcctg cgaaacttaa caaagggtat acacgccagt tcctttctgt
      901 ggatctcggc aaggccgacg gcgcgttcac ctgtgaaaca caccctattt ccgacgagat
      961 ggtggagaag tttaccggcg gacgcggttt cggccttcgt atcctctggg acaaggtcac
     1021 ttcaaagagc aagtggaacg acccggaaaa cgcgatggtc atctccggag ggccgctttc
     1081 cggcaccacg caatatccgg gcatgggcaa gttttacgcg gtattccttt cgccgcttac
     1141 gcaacagacg tacaacagca atgcaggagg gtacttcggg ccattcctga aggctgccgg
     1201 gtttgacgcc ctttctgtat ctggcaaagc gcagaaagac gttgtagtgt atatcgacgg
     1261 cgatgaaggc gcggtacgta tcttcgaatg ggcgcctgag aaccgtaact cctataccgt
     1321 cacggaagcg cttcatgagt tcttcgcgga gaacgaagaa gacaagcgca acatctcggt
     1381 tgtctcggct ggagaagctg cggaacacag ctggtggggg gctatgaacg tcagtttcta
     1441 cgacccaaga agagcgcttc ctcgcttgaa gcaagctgga cgcggcggcg gcggcactgt
     1501 gctcagggac aaaaagatcc tcgcgctcgt cgtcaaaaag aagggctttt ctggtaccga
     1561 aaacgccccg gctgatctgt ccactttgca gaaagtcggg acaaaactcc accgtgaaat
     1621 tcgccagaac gacgacgtac agtgtctcat gagaaaagtg ggcacagcgc accttaacct
     1681 cgttatgaac gactacgaca ttcttccggt gaacaacttc aaattcggaa gaaacgagga
     1741 cattcacaat atcgctcctg acgcctacga aaaactattc acccagggat acgccgacgg
     1801 ctgctggtac ggctgttcaa tggcctgcgc aaaagctgtc gacggttttg aactgacgac
     1861 ggggccggat aaagggaaaa aggttaccgt agacggnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnngtag gcaaaggcgt cagaaagatg aaagaaattt tttccgaaaa
     2041 gtacggagct gaccggactg ttatggaacg aatcggcatg gagggacagg ggcttgaagt
     2101 ctcccagtac cgatgccagg agtcggttgc ccaatggggc ggttatttcc ttacgctcaa
     2161 ggggcctcag catgacgaag catgggttat cttcatggac atggtgaata aacagctccc
     2221 aacctttgaa gataaagcgg aagcgctctt ctacttcccc aacttccgtc tgtggttctc
     2281 gctcaccggc gtctgcaagc tcccctggaa cgacgtggaa ccttcggaca accgaatccg
     2341 ctacaagggt atcgaggccg ccaaggtccc ggagcacgtt cagaactatc ttgacgtcta
     2401 taccgccgta accggcaagc cgctgagcaa agaggagatg atcctccagt cggagcgtgt
     2461 ctacaatttc gaaagggttt tcaacctccg catggggaag ggaacgcgcg cggaccataa
     2521 cattccggac cgcgccctgg gccccgtctt tgaagacgag tggaacgcgc gtcctgagta
     2581 cttcgacggc aagctcagtg aagcgggtat ttcccccgaa ggtctgcctt tgaaagaaaa
     2641 gatgacaaaa cttctcgaat tcagacgaag cgaatgggaa aaactcgtcg acgccgtcta
     2701 caagcgcaga gggtggaatg cgaacggagt tccgacactg gagacactca aacgccttgg
     2761 catcgacttc cccgaagtcg tcgacgtcgt caaggcacac cagtaaacac aagggccggg
     2821 cggtggcgga ccaatgatca gggtcaacgg cagggagatg gagtggcgcc ggggcatgac
     2881 tatacaggat gtccttgatg agtgcggcta taccttcaga atgatcgcag tgtggatcga
     2941 cggtactccc taccgcagag aacatttcgc caccgccgtc gttcctgaca gagcggaagt
     3001 acaggctctt catatgatca gcggaggtta gacactgtaa tgctcttcgg aaaagaaccg
     3061 acaggattta tcttcgatgt gaaacggtac tctatccatg atggaccggg gatccgcacg
     3121 acatttttct ttaaaggatg ccctctttcc tgttggtggt gccataatcc ggaaggcatt
     3181 tcacctcttc cggcgcccac gtatcatgga aacaggtgta tagcctgcgg acgatgcgtt
     3241 cgtgaatgtc ccaatggagc ccgaagcttc ggccccgaag gaattgcgct ggactcttcg
     3301 aagtgtgtcg agtgcaggat atgcgagcaa gtttgtccta cgcgtgctct cgagttcgtc
     3361 ggcaaagaaa tgactgtgga agaaattgtt cgcctcgcga aaaaggatct tgctttttat
     3421 gacgaatcag gcggaggtgt cactttctcc ggaggagagc ctttgatgca ggccgagttt
     3481 ctcatcgcgt gtctggaagc gctccgctct ctggaaattt ctacggcagt ggatacttcc
     3541 ggcttctgtc gcgaggagga tcttcttctc gccgcggaag aggcggatat ttttcttttc
     3601 gacatcaagc tgattgaccc gaagctgcat gaatactata ctggtgtagg taatgaaaga
     3661 attttagcca atctgaggag cctcgacaaa aagctcgtgg aacagggaag aggaagcatc
     3721 aatatcagga ttcccctcat tcccggtgtg aacaattctc cggaaaatct ggaggccgtg
     3781 attgcatgcg ttctttctct acgaagcgtt tcgggcgtga atattttgcc atatcactct
     3841 atcggatccg gcaagtacaa aaaccttgga ttggagttca aaatggggca gacgcagccc
     3901 ccgtcggatt atgaagtttc acaggcgctt tcactgtttg catcgcgggg gatccacgcg
     3961 gtgaaaggag gataagagaa atgaacgaga gaattgcacg tctgagaaat gagagtttcg
     4021 aaacatatcc gagtatttca atcgaacgag cccgtttgac caccgatttc tacagggcca
     4081 atgaaggcaa atacccggtg cccgtcatga gggcgcttaa cttcaaaaat ctttgtgaac
     4141 aaaagacgct ctacatagga aaagatgagc tgatcgtggg ggagcggggc cctgctccaa
     4201 aggcagtctc caccttcccg gaactgaatt gccatacggc aagagacctc gaaatcctga
     4261 acacgcgtcc tatgaccgga tacagggttc acgaagatga tattcgcatc tacacggaag
     4321 aagtcgtgcc ctactggacc ggccgcactc tacgggacag gattttttca aacctccccg
     4381 aagaatggct tgcgctctat gaagcggggt gtttcaccga attcatggag caacgagcac
     4441 cgggacacac agcgctcgac ggaacgatct attcaaccgg actgctcgac atgaagaaaa
     4501 aaatcgcctc cgctcgcgaa aggcttgatt ggatcaacga tcctcacgcc actgaaaaag
     4561 acgaggagct acaggcgatg gatattgcct gcgacgccgc aattatcttc gcacgacgtc
     4621 atgccgatct cgccgaggag atggcagcat ccgagaagga cttcgtccga aaggcagaac
     4681 tcctcaagat cgccgacgtc tgccgtcatg ttccggcgaa cgctccgcgc gattttcacg
     4741 aggctatcca gatgtactgg tttacacatc tgggcacaat caccgagttg aatggctggg
     4801 acgccatgtc cccggggcat ttcgacctcc atctccgccc attttacgag aagggaatcg
     4861 cggacggttc cctcgaccgc gagaaagcga aagaacttct ctcctgcttc tggatcaagg
     4921 tcaacaatac tccggcgcct ccgaaggttg gcgttactgc tgcggagagc ggaacataca
     4981 acgacttcac ccagatcaac ctgggcggaa tagccgagga cggaagcgac ggctcaaacg
     5041 acgtctccta cataatactc gacgttctcg acgagcttca gctgctccag ccccaggtca
     5101 gcgtacacat atcttcaaaa acttccgaac gtttccttcg ccatgcctgc aaggtgatgc
     5161 ggcgaggctc cggctatcct tcggcattca actccgaggc tgtcttcatg gagcagttgg
     5221 ggatgggcaa aaccgtcgaa gacgcccgta ccggaggaac aagcggttgc gtcgaaaccg
     5281 gctctttcgg caaggaagcc tacatacttc acggatactt gaacgttcca aagatcatgg
     5341 aactcgctct aaacaacgga accgatcctc tctccgggaa gcgcataggg ttggagacag
     5401 gcgccccgca gactttcacc tgctacgagg agttctatca agcgttcgag aaacagctgc
     5461 actatgtagt cgacacaaaa atacgtacgg ataactacat ccaaaggatg ttcgcacggc
     5521 atgctccggc tccgtacctc tccgtcctca ttcacgactg catcgagaac ggcagagact
     5581 attacgacgg cggtccccgc tataatacgg actacatcca gtgctgcgga attggtacgg
     5641 tcacggatag tttctccgtt ctcaagaagc atgtcttcga agaaaagact gtgacgcttg
     5701 ccgatcttct tcgcgcgctt cacgagaatt gggaaggctg ggaggagatg cgccagttta
     5761 tctggaaccg caccccgttc ttcggtaacg acgacgaaga agccgactcg attatgcaga
     5821 aggtgtatgc gtcgcttctg aacgccatcg acgggaaaca ctctccaaag ggacctacat
     5881 accacgtgaa tatgctctcc acaacgtgcc atatctattt tgggaaaaaa ctgggcgcta
     5941 caccaaacgg acgttttgcc catgcgccgg aatcagacgg gacttcgcca tctcacggcg
     6001 ccgacaggaa tggacctacg gcggtcgtca agtccctcag ccgtatggat cagctcaaat
     6061 caggaggcac gctgcttaat cagcgtttcc ttccctcggt tctgagcgga gactccgggc
     6121 ttgagaaaat gggacaactc atacggacct actttaaact cggaggacat catatccagt
     6181 tcaatgtagt cgatacggag acactacgca aggcgcagaa gaatccggac gagtaccgca
     6241 gtcttcttgt tcgagtggca gggtatagcg actatttcgt tgaccttgat caataccacc
     6301 aggaagaaat catcgcccga acagcgcagg agaacttata acacattact ccgtgcgctt
     6361 ttttaaggtg aagggagggg atattcgtcc ggtgaaaaaa taggcataac catcacgcga
     6421 cacaagaggc atcatcgatg agtaccgcaa aaacgaaagg agtgctgaca taatgcagaa
     6481 agcgaaaaaa tttgtcacct tgtgtatcct ctccgccctt gttttgttcc tcatcgttcc
     6541 gaacatggct tccgcagctc ccgagatgac tctgcgcttc gccgggcagg tccccttgga
     6601 gcacaccgcc accaaactga tgcatcaggt tgcggatgaa gtaaaagaaa agacgaacgg
     6661 acgcatcgtc gttgaagtgt atcctgcaaa ccagctgggc gactacacgc ttgtctacga
     6721 agagcttatc cgcggaacga tcgatatggc tgccatatcc gttcccagcc agttcgatcc
     6781 tcgccttgaa gtaacctaca tcaactgttt tgtcaggaat tttgacgaag cgaaatccgt
     6841 ttttgctccc gacggctggc tcttcaagaa gatggacgaa ctgagcgcgc gccttggcgt
     6901 taagcttctt ggattccagg tcgaaggctt tatcggtatc ggcagcacca agccggtgaa
     6961 cgagccgctg aatccgaacg tggacaaggg cgtactcgtc cgcattccca acatggacgt
     7021 ctacaagctc ggcgcggaag caatgggcta ccgtaccgtc accgttccct gggctgaagt
     7081 ctacacagcc ctgcagacag gcgtagccga cggcgttgac gggatgaccc cgacagctgc
     7141 gtacacaatg ctcaaggatg ttctgaagta ttggtatgac ttgaattact cggtcgaaaa
     7201 cttcaactac atgatcagca tgaaaacatg ggagaaactc tcggaagctg atcgtaaggt
     7261 tatcgccgat gcgtgcgcga aggtcactga aatgagcatc gaacttgcga aacaagacca
     7321 ggaaaagcac cttcagctta tgcgcgacgc cggcatcgag gtacatacct ataccgcaga
     7381 agagctgaag cccatttttg aaaaagtatc cgccacttgg ggacagcttt ccgataagtt
     7441 tactcctgaa ctaatcgaag agttcaagaa agaatacgca cccaaataga caattcaaca
     7501 accccgtatc ctttcgagta gggacacccc ctactcgaaa ggatacgaga aggaaaggag
     7561 atctttatgg cggacaacga gaccatcggc tgttttcctg aaaatcgtcc cgaaaaacag
     7621 agcaaagtag atttaatcct gctcttcttt ttttctatag tttctgtttc ctgctctctt
     7681 ctcttgtcta tcatcatatc tgccgctgca tttacacggt atatcatcgg cggcgacctc
     7741 tacggcttcg aggaatgggt ccgtctgttg gccttctggc tttattttat ggggggagca
     7801 tacggagctt acaacgatac aca
//
LOCUS       DAOPKU010000068         7798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_9971_length_7798_cov_5.059021, whole genome shotgun sequence.
ACCESSION   DAOPKU010000068 DAOPKU010000000
VERSION     DAOPKU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9971_length_7798_cov_5.059021"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1909)
                     /locus_tag="PK849_03340"
     CDS             complement(<1..1909)
                     /locus_tag="PK849_03340"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPE65190.1"
                     /translation="MKKDGLTAKKNGAFRNMKLGSRIILAVGALLLPAILIFAGIVLR
                     RIDADATASIEALRVSETERIRSDLRGYVDIAWRILSDKYEQSTQTESIIQSYGDQLR
                     SVVDIAENIVRQIMKSAEEGQFSREEAKRQAMIALRALRFDDGLGYVWVNDTSLPYPS
                     FLMHPTQPELDGVSMKGPDFDKAMGSEKNIGKSILEACALDGEGFVQYLWSKPTRDGL
                     IPDIPKLSYVRSIKEWGWIIGTGVYVDDAREYAVRQALDIIANMRYAGGAGYFWVTDT
                     HRPNPRPVIHPTLDAAENELPGITPDDKYYDTARKLVEEVGVQAANVCEANGEGFVQY
                     LWPRIGADGALELAPKETFVRLFEPLQWIIGTGVHLDDVDRAVALRQAESKRQRDIVL
                     RGTLFAALLLVALAFVLLYFVVRGIVRPIVQLTAAADMVASGDMETPLPDSGPSEIRS
                     LSRSFNRMTEELKERMTALRESEGRFRRLAENSPDMIFRISLRDGRYEYISPAVTRLL
                     GYSQEEAYTTPFFARKIIHPDFEQFFESAWEDLQRLEAPPTFEFKVLAKSGEEFWVYQ
                     RNVLVCGDDGEPVALEGLVTDISDRKAAEAALDNYREHLEELVRDRTAELRIAKEAAE
                     QANRTKSAFLAN"
     gene            complement(1935..2423)
                     /locus_tag="PK849_03345"
     CDS             complement(1935..2423)
                     /locus_tag="PK849_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65191.1"
                     /translation="MDMRMPVMDGYEATKRIKASEGGSSVPVIAVTASAFEDDVQNVL
                     KSGADICIRKPLSPEDVYEALGKLLDIRFHYADEPRTADTEQEPPPSMEALAALPEEL
                     IKAMRRAVEDGDIDELHTLVDRVREMNREVAEGLQKLVRQYDYAALSSLLSDGDRESG
                     SS"
     gene            complement(2423..4468)
                     /locus_tag="PK849_03350"
     CDS             complement(2423..4468)
                     /locus_tag="PK849_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65192.1"
                     /translation="MEKILDIVFNLGMLASLSILSGFIGADGERRARKLLMQGALYGT
                     ASLLGMLHPLVLAPGVIFDGRSVMLSVCGFFFGPAAAALAASMALVLRVHQGGSGAVM
                     GVSVIVSSALIGALFHRMHYSRSRRASARLLYLMGLLVHAAMVLLMLTLPEDQSMKSI
                     RLLALPVMIAYPLTTLFIGRILSEFDAHRRMGESLKLSESNLRRSNEELAANLNKLMK
                     REKELRFANAVLLTQQETSLDGILVVDGEGEIVSFNRRFTELWDIPDDIAASRDEKKV
                     LKRATERLLHPADFLSKVNFLYQHPHEKSRDEIELTGGTVFDRYSAPMLGLAGEYYGR
                     IWYFRDITERKRAEEEVRKHREHLEELVRERTNELEAAKEAAERANMAKSAFLANMSH
                     EIRTPLNAILGFGTILERDLSLSETQADQVRTINRSGRHLLKLINDILDMSRIEAGRL
                     ALHNVPFCLQDLLDDLEAMFRVRAEEKGLSLSLERDAKLPGNVVGDEAKLRQVLVNMT
                     GNAVKFTKQGGVTLRARMEAAVETGPRLVLEVEDTGPGIPPEELEHIFDAFRQSEAGR
                     EAGGTGLGLSISKRLVDLMGGELAVRSTLGEGSCFTASVPVEPSEETPAPPKTPPRRV
                     AGVERNEAEGPLLIVDDVKENRDILKALLAPLGFALREAENGREAFGCSRRNAPAPC"
     gene            complement(4643..5950)
                     /locus_tag="PK849_03355"
     CDS             complement(4643..5950)
                     /locus_tag="PK849_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65193.1"
                     /translation="MKRNGMKATVMVVDDTPANLDLLDRMLRAAGYRVLAFPRGRLAL
                     EAAIEIPPDLIVLDVLMPDMDGFEVCRRLKATPRLASVPVLFISALGDTSDKLRAFAC
                     GGADYITKPFQEEEVLARAGAHLEIAALQRRLREQNENLERLVAERTQELASERARAL
                     RSFAERIRGARWGEERSLGLDAAVSEFDALSEAIEELAEKTYTREELRARYIGDMIRA
                     EEKGRKRFARELHDGPLQSAAAAIRRVQMAREAAAANPSGELGALLDTAEEIMRSAAD
                     ELRGFCEDLSPSWISLGLVSAMDENADSLSRTHGGAIIEATTDETLDSLPEEHVLALV
                     RIMQEAVSNSVRHGRARSIQVRVRDEGSAVRFSIRDDGKGFDAGEHPGTEYERLRTSG
                     HRGLANISERVRLLRGTMRLESRPGEGCGIDIVFPDPRARAAD"
     gene            complement(5947..>7798)
                     /locus_tag="PK849_03360"
     CDS             complement(5947..>7798)
                     /locus_tag="PK849_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65194.1"
                     /translation="ERNILRDVLESPLSGFWEMYFKSDAAFLSPKFRNLLGYGEEELP
                     GTSMLCHRIVHPEDLPGTLHLLQEHIQSKGKTPFSNEIRYVRKDGLILWAICAGRVVE
                     WAEDGSPVTMVGCYIDITERKRAEEEMHLLATRQHAMEVAKESAEAANKAKSAFLANM
                     SHEIRTPLNAILGFGAALERDPSLSEKQAEQVRTINRSGRHLLRLLNDILDMSRIEAG
                     RLTLTQAPFCLHDLLDDLESMFRLRAESRGLHLDMEYGEDVPRYAVGDEAKLRQVLLN
                     LLSNAVKFTKEGGVAIRVHADALNPGKEFVSLLIDVEDTGPGIDLIELERIFEPFRQS
                     EAGREAGGAGLGLAISRQLAELMGGRLTVESRQGDGSRFSVLLPLRRSEEGAGPKQDP
                     RRVVGVENGGKEYRILVVDDKKENRDVLRALLEPMGFALKESANGTEALKVFGEWSPH
                     AILMDMRMPVMDGYEATRRIKADLKGAAVPIIAVTAFAFEEDEQKAFQAGVNGYLRKP
                     FRPDELFLALGKALGLRCIHAEDSRGGETPLTSEALTVIPAEMRAAMRAAVKSGDMAG
                     LREQITAARAIDEATAEKLLALARRYDYERLQSLLSSAQQCEANEGRTRE"
BASE COUNT         1323 a         2486 c         2380 g         1609 t
ORIGIN      
        1 tgttggcgag gaaggcgctc ttggtacggt tggcctgttc cgccgcctct tttgcaatcc
       61 gaagttcggc ggtgcggtcc cggaccagct cttccaggtg ctcccggtag ttgtccaggg
      121 cggcctccgc cgctttcctg tcgctgatgt cggtgacgag gccttcgagg gcaaccggtt
      181 ctccgtcgtc gccgcagacg agcacattgc gctggtacac ccagaactcc tctcccgatt
      241 tggcgagcac cttgaattcg aacgtcggag gcgcctcaag gcgctgaaga tcctcccacg
      301 cgctttcgaa aaactgttcg aaatcggggt ggatgatctt cctggcgaag aagggcgtgg
      361 tatatgcttc ctcctgggag tagccgagca gccgggtgac ggccgggctg atgtactcgt
      421 aacggccgtc gcgcagcgaa attctgaaaa tcatgtccgg agagttctcc gccagcctgc
      481 ggaagcgtcc ctcgctctcg cggagggcgg tcatccgctc tttcagctct tccgtcatcc
      541 ggttgaagga gcgggagagc gagcgaatct cgctcggacc ggagtccggg agcggcgttt
      601 ccatatcccc ggaggccacc atgtcggcgg cggcggtaag ctgtacgatc ggccggacga
      661 tgccccgcac cacgaaatag agcagaacga aggcgagtgc gacgagcagc agcgcggcga
      721 agagcgtgcc gcgaagcaca atgtctcgct gccgtttgct ctcggcctgg cgaagagcga
      781 cggccctgtc gacgtcgtcc agatgcacgc ccgtcccgat tatccattgg agcggttcga
      841 agagccgcac gaacgtctct ttcggcgcga gttcaagggc tccgtccgcc cctattcggg
      901 gccacaggta ctgcacgaac ccctcgccgt ttgcttcgca cacgttggcc gcttggactc
      961 cgacctcttc gaccaacttg cgcgcggtat cgtagtattt gtcgtccggc gtgatccccg
     1021 gcagttcatt ttcggcggcg tcgagggtcg gatgaatgac cggcctcggg ttgggccggt
     1081 gcgtgtcggt gacccagaag taccctgcgc ctccggcata acgcatgttt gcgatgatat
     1141 ccagcgcctg ccggacggcg tactcccggg cgtcgtccac gtagaccccg gtgccgatga
     1201 tccaccccca ctcctttatc gatctgacgt aggagagttt tggtatatca ggaatcagcc
     1261 cgtccctggt aggtttggac cacaggtatt gcacaaatcc ctctccgtcc agcgcgcacg
     1321 cttcgagtat cgatttgcct atgttctttt cgctgcccat cgctttatcg aagtccggac
     1381 ccttcatcga gacgccgtcc agttcgggtt gggtagggtg cataagaaac gaagggtagg
     1441 ggagagatgt gtcgttgacc cagacgtagc cgaggccgtc gtcgaaacgc agggcccgaa
     1501 gggcaatcat agcctgccgt tttgcctctt cacgggaaaa ttgcccctct tccgccgact
     1561 tcatgatctg ccggacgatg ttttccgcga tgtcgaccac cgagcggagc tggtctccgt
     1621 acgactggat gatagattcg gtctgcgtcg actgttcgta cttgtcggag agaatgcgcc
     1681 atgcgatgtc gacgtagccg cgcaggtcgg agcggatgcg ttcggtctcg gagactcgca
     1741 gcgcctcgat ggaggctgtt gcgtcggcgt cgatgcggcg gaggacgatt ccggcgaaga
     1801 tgaggatggc aggcaacagc agcgccccga cggccagaat gattcggctg ccgagcttca
     1861 tgttccggaa cgcgccgttt ttcttagcgg tcaatccgtc ctttttcatt cgagttcctc
     1921 ctgactgcgc ccgatcatga gcttccgctc tcccggtcgc cgtccgagag aagagaagaa
     1981 agcgccgcgt agtcgtattg gcgtacgagc ttctgcaacc cttcggcaac ttcccggttc
     2041 atctcccgca cccggtcgac gagcgtgtgc agttcgtcga tatcgccgtc ttcgacggcc
     2101 cttctcatcg ccttgatcaa ctcttccggc agcgcggcca gcgcttccat ggaaggcgga
     2161 ggctcttgtt cagtatcggc tgtccgcggc tcgtcggcgt agtggaagcg tatgtcgagc
     2221 agcttcccca gcgcctcgta cacatcctcg ggactgagcg gtttgcgtat gcagatatcg
     2281 gcgccgctct tcagcacgtt ctgaacgtcg tcctcgaagg cgctcgccgt caccgcgatg
     2341 acgggaaccg aacttcctcc ttcgctcgcc ttgattctct ttgtggcttc atagccgtcc
     2401 atcacgggca tccgcatatc catcagcacg gcgcaggggc gttcctcctc gaacagccga
     2461 aggcctcccg gccgttttcc gcctctctga gggcgaagcc gagaggagcg agcagcgcct
     2521 tcaggatatc gcggttctct ttgacgtcgt cgacgatgag caacggcccc tccgcttcgt
     2581 tgcgttccac gcccgcgacg cggcgcggcg gcgtcttcgg gggggcgggc gtttcctccg
     2641 acggttcgac cggtaccgac gcggtaaaac agcttccctc gccgagagtg cttcgaacgg
     2701 cgagctctcc gcccatcagg tcgacaaggc gcttgctgat cgacagtccg agcccggtgc
     2761 cgcccgcctc gcgtcctgct tccgactgac ggaacgcgtc gaaaatatgc tccagctctt
     2821 ccggaggaat gccggggccc gtatcctcca cctccagcac aaggcgcggg ccggtttcca
     2881 cagcagcttc catgcgggcg cggagcgtta ccccgccttg cttggtgaac ttgacggcgt
     2941 tcccggtcat gttgacaagc acttggcgca gtttggcctc gtccccgacc acgttgccgg
     3001 ggagcttcgc gtcgcgttcc agggagagag agaggccctt ttcttccgcc cgcacgcgga
     3061 acatcgcctc caggtcgtcg agaaggtctt gaaggcagaa cggcacgttg tgcagcgcaa
     3121 gacgcccggc ctcgatcctc gacatgtcga ggatgtcgtt gatcagtttc agcaggtgcc
     3181 ggccgctccg gttgatcgtg cgcacctgat cggcctgcgt ttccgagagc gagagatcgc
     3241 gttcgagaat cgtgccgaat ccgaggatgg cgttgagcgg cgtacggatt tcatggctca
     3301 tattcgccag gaaggcgctc ttcgccatgt tggcccgctc ggcggcctcc ttggccgcct
     3361 caagctcgtt cgtccgctcc cggaccagct cttccagatg ctcccggtgc tttcggactt
     3421 cctcttccgc acgtttgcgc tcggtgatgt cccggaagta ccagatacgg ccgtaatact
     3481 ctccggcaag gccgagcatg ggcgcggagt accggtcgaa caccgtgccg ccggtcagct
     3541 cgatctcgtc ccggctcttt tcatgagggt gctggtataa gaagttgact ttggagagaa
     3601 aatcggcggg gtggagcagg cgttccgtcg ctctcttcaa gaccttcttc tcgtcgcggg
     3661 atgcggcgat atcgtcggga atgtcccaca gctccgtgaa gcgacggttg aaggagacaa
     3721 tctccccctc gccgtcgacg acgaggatgc cgtcgagcga cgtctcctgc tgtgtgagga
     3781 gtacggcgtt cgcgaagcgc agctctttct cccgtttcat gagcttgttc agattggcgg
     3841 ccagctcttc gttggatctg cggagattcg actcgctgag cttgagcgac tcgcccatcc
     3901 ggcgatgcgc gtcgaactcc gagagaatcc tgccgatgaa gagcgtcgtc agcgggtacg
     3961 cgatcatcac ggggagggcg aggaggcgga tgctcttcat cgactggtct tcgggaagtg
     4021 tgagcatcag cagcaccatc gccgcgtgca ccagcagccc catgaggtag agaagacggg
     4081 cggaggccct gcgcgatctg ctgtagtgca tgcggtggaa gagcgcgccg atgagggcgg
     4141 aggagacgat gaccgagact cccatcacgg cccccgagcc tccttgatgg acacggagga
     4201 caagcgccat cgacgccgcg agagcagcgg cggccggacc gaagaagaag ccgcagacgc
     4261 tcagcattac cgaccggccg tcgaaaatga cccccggcgc gagcacaagg gggtggagca
     4321 tccccaagag agaggccgtt ccgtagaggg ctccctgcat caggagtttt cgcgcccggc
     4381 gttccccgtc ggcgcctatg aagcccgaca aaatgctcag cgaagcgagc attccgaggt
     4441 tgaagacgat gtccaggatc ttctccatag gccgtttcag ctcctttaca gaaaatatcc
     4501 ttgtatgcgg gggcgttctt cgaaggcgcc cgccgttcgg attcttctct gctttcatta
     4561 ttataaagta cgagcgtcaa aaattaatgt agaacaatgc gttctgtttg agcgcggaga
     4621 gaaggcggta gcctcctccc tgtcagtccg cggcgcgcgc gcgcggatcg ggaaagacga
     4681 tgtcgatacc gcacccctcg ccggggcgcg attccagccg catcgtcccg cggagcaggc
     4741 ggacgcgttc gctgatgttg gccagccctc tatggccgga ggtccggagg cgttcgtact
     4801 ccgtcccggg atgttcgccc gcgtcgaacc ccttaccgtc gtcccggatg gagaagcgga
     4861 cagcgcttcc ttcgtcccgt acgcgtacct gaatcgagcg cgctcttccg tgccggacgg
     4921 agttcgacac ggcttcctgc attatgcgga cgagcgccaa tacatgctcc tcggggagcg
     4981 agtccagcgt ctcgtcggtc gtcgcttcga tgatcgcgcc gccgtgcgtg cgggagagcg
     5041 aatcggcgtt ctcgtccatc gcgctgacga ggccgaggct gatccaggac ggggagagat
     5101 cctcgcagaa cccccgaagc tcgtccgccg cactcctcat gatctcctcc gcagtatcca
     5161 gaagagcgcc gagttcgccg gacggattcg ccgctgcggc ttccctcgcc atctgaactc
     5221 gtctgatcgc ggcggcggcc gactgaagcg gtccgtcgtg cagctcgcgc gcaaagcgtt
     5281 tgcgcccctt ctcctcggcg cggatcatgt cgcctatgta ccgcgcccgc agctcctccc
     5341 tggtgtatgt cttttcggcc agctcttcga tggcttccga cagcgcgtcg aactcggaga
     5401 ctgcggcgtc gagccccagg ctgcgctcct cgccccatcg cgctccgcgt attcgctccg
     5461 cgaaactccg cagcgcgcgg gcgcgctccg aagcgagttc ttgtgtacgc tcggcgacca
     5521 gccgctcaag gttctcgttc tgctcccgca gtcgacgctg cagcgccgct atttcgagat
     5581 gcgcgcccgc gcgcgccagc acctcttcct cctggaacgg ttttgtgatg taatcggccc
     5641 ctccgcaggc gaaggcgcgg agcttgtccg acgtgtcgcc cagcgcgctg atgaagagca
     5701 cgggaacgga ggcgagccgg ggggttgctt tcaagcggcg gcagacctcg aagccgtcca
     5761 tatccggcat caaaacatcg agcacgatga gatccggcgg gatctcgatc gccgcttcca
     5821 gcgccagccg accgcggggg aaggcgagca ctctgtatcc cgcagcccgc agcatgcggt
     5881 cgagaaggtc aaggttggcg ggcgtatcgt ccaccaccat gacggtcgct ttcattccgt
     5941 tccgcttcat tcccgtgttc tcccctcgtt tgcctcgcac tgctgcgccg acgagagaag
     6001 cgattgcagc ctctcgtagt cgtaccgtct ggcaagcgcc agaagttttt cggcggttgc
     6061 ttcgtcgatc gcgcgcgcgg cggtgatctg ttctcgcaag cccgccatgt cgcccgactt
     6121 gactgctgcg cgcattgcgg cgcgcatttc tgcgggaatg acggtcagcg cttcgctcgt
     6181 cagcggcgtt tcacctccgc gggagtcctc ggcgtgtatg caccggagtc cgagagcctt
     6241 tcccagcgca agaaagagct cgtccggcct gaacggtttg cgaaggtagc cgttcactcc
     6301 cgcctgaaat gctttctgtt cgtcctcctc gaaggcgaag gccgtgaccg cgatgatagg
     6361 aaccgccgcg cccttgaggt ccgctttgat tcgacgggta gcctcgtacc cgtccataac
     6421 cggcatgcgc atatccatca gaatcgcgtg cggcgaccac tcgccgaaaa ctttcagcgc
     6481 ctccgtaccg ttcgcgctct ccttcagcgc gaatcccatg ggttccagca gcgcccgcag
     6541 cacgtcgcga ttctcctttt tatcgtccac cacgagtatc cggtactctt ttccgccatt
     6601 ctccacgccg acgacgcgcc gaggatcctg tttgggaccg gcgccctcct cgctgcggcg
     6661 gagcggcagc agaacggaaa agcggcttcc gtcgccctgc ctgctctcga ccgtaaggcg
     6721 cccgcccata agctcggcga gctgcctgct gatggcgagg ccgagtccgg cgcctccggc
     6781 ctctcttccg gcttccgact gacggaaggg ttcgaagatc ctctccagtt cgatcaggtc
     6841 gatcccgggg ccggtgtctt cgacgtcgat cagcagactc acgaactctt tgccggggtt
     6901 cagcgcatcc gcgtggacgc gaattgcgac gcccccttct tttgtgaact tcaccgcgtt
     6961 cgacaacaga ttgagcagta cctgacgcag cttcgcctcg tcccccacag cgtagcgcgg
     7021 cacgtcttcg ccgtactcca tgtccaggtg aaggccccgc gattcggcgc gcagcctgaa
     7081 catcgattcc agatcgtcca gcagatcgtg gagacaaaaa ggagcttgcg tcagcgtcag
     7141 ccgtcccgcc tcgatccggg acatatccaa aatgtcgttg agaagacgca gcagatgccg
     7201 tccgctcctg ttgatggtgc gcacctgctc ggcctgtttc tcggagagtg agggatcgcg
     7261 ttcgagcgcc gcgccgaagc cgagaatggc gttgagcggc gtgcggattt cgtggctcat
     7321 gttcgcaagg aaggcgctct tcgctttatt cgcggcctcc gcgctctcct tggcgacttc
     7381 catggcgtgt tgacgcgtgg cgagaaggtg catttcctcc tccgcccgtt tgcgttcggt
     7441 gatgtcgatg taacatccca ccattgtgac gggagagccg tcttcggccc attcgaccac
     7501 ccgaccggcg cagatcgccc agaggatcaa gccgtccttg cgcacatacc gaatttcatt
     7561 ggagaaagga gtttttcctt tgctttgtat gtgctcctga aggagatgga gcgtgccggg
     7621 caaatcctca gggtggacga tccgatggca gagcatcgat gttccgggca gctcctcctc
     7681 tccgtacccc agcagattcc ggaacttggg actcagaaac gccgcatcgc ttttgaagta
     7741 catctcccag aaaccggaga ggggggactc aaggacgtct ctcaggatgt ttcgctcg
//
LOCUS       DAOPKU010000069         7758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10039_length_7758_cov_4.449305, whole genome shotgun sequence.
ACCESSION   DAOPKU010000069 DAOPKU010000000
VERSION     DAOPKU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10039_length_7758_cov_4.449305"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PK849_03365"
     CDS             complement(<1..633)
                     /locus_tag="PK849_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE65195.1"
                     /translation="METLLLFALLIVLIATSIPIGIALGLATAIMLHFTTNVSLIMIA
                     QKSFSSLDSFPLLAIPFFILAGALMSYGGISRRLVALAESLVGFIIGGLAMVTVLACM
                     FFAAISGSGPATVSAIGSFMIPAMKERNYDAGFASALTAAAGTIGVIIPPSIPFVLYS
                     IVSGTSVGDLFLAGVVPGVIIGVLLMLNCYVIARKKGYLGDGKVLTLADAS"
     gene            complement(633..1112)
                     /locus_tag="PK849_03370"
     CDS             complement(633..1112)
                     /locus_tag="PK849_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE65196.1"
                     /translation="MRKAFDRVEEVFLVGSLAFNVVLIFIQVVMRYVFQNSLSWSEEL
                     ARYIFLWQTWVGASYAVRMRRHFRVEMLVDLMKGRLRKWYELVVLLVWCAFALFIAWQ
                     GSVLTAFLIKRWQVSAAMQIPIAWAYASVPAGCAMMAIRLVMEMKDLLRDLRKGAAC"
     gene            complement(1207..2196)
                     /locus_tag="PK849_03375"
     CDS             complement(1207..2196)
                     /locus_tag="PK849_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE65197.1"
                     /translation="MCGVAALAVLLAVVLTGAAFAAPEYVIKVGYILPENQSDHIIMR
                     DVFKKDIEEKSGGRISVELYPNAQLGGDRELIESVQLGTVQVAIPATSALAGFDKRFQ
                     VFDLPFLFKSKETAFKALDGELGHKVDELLKPLGMRNLGYGENGYRHITNSRGPVHKP
                     EDLKGIKLRTMENPLHLAFFKMLGANPTPMSFGELYTALQQGTVDGQENPVVLVYTSK
                     FYEVQKFYSLTGHVYSATMLVANDDFFASMPEDLWKIVEDAGKRYVVEQRALAEVQEQ
                     EFLEELKKTGLQINELTPEEKQLFIDATLPAYDQFKDVIGAELVELAKKANEE"
     gene            2631..3776
                     /locus_tag="PK849_03380"
     CDS             2631..3776
                     /locus_tag="PK849_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPE65198.1"
                     /translation="MHPHLFTPGPVEVPHSVRAAGAAPLVGHRSEAYFALLEEIVALL
                     KKLLRVSQPVITLPSSGTGALEALAANLLDPGDAVISFSCGAFGDRFREIASRRGATI
                     LPVDIPRGRTATPSDVRLALEQHPGAAAMLFTHNETSTGALTDLRACTSALPEDGPLV
                     LVDAVSSFGAVPCFPEEQRIDGLASCSQKGLLTPPGIGIVALSERGWKRAASRPSPSY
                     YFDLPLHRRFLEKSRPQNPYTPPVTLLYSLAEALRFLDGIGFERRFADVERTARTFET
                     ACESMGLSLFVKEQELRSPAVTAVTVPEGRAEEMKRALAELGVEVAEGQGKEPMLRVA
                     HYSPGGWPELCLVAGSLYGAGRACGLELSPDFLEPAWNCWKKGACTQ"
     gene            3773..5419
                     /gene="serA"
                     /locus_tag="PK849_03385"
     CDS             3773..5419
                     /gene="serA"
                     /locus_tag="PK849_03385"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPE65199.1"
                     /translation="MKVLVTDTISEAGVALLRKEPDLKVEVRLDLSREELLEAVADAD
                     GMLTRSGTPLDREVLDAAKKLKAVARAGVGVDNIDIAAASRRGVVVINAPTGNTLAAT
                     EHTMAMLLSLVRKVPQAWNSVASGEWKRSRFMGMQLSGKRLLIVGLGRIGTQVALRCR
                     AFGMEIMAFDPYVSKEKADRAGVQLVDDLKGGMALADVITLHVPLTRESRGMLDAATL
                     RACKRGAYLVNCARGGLVDEEACAEAVRSGILAGTAFDVFDGEPVRADHPLLASDIRG
                     KVILTPHIGANTEEAQSAVAVIAASNLAAALLGKPYEHAVNLPFSEQLLSDGSRAYLS
                     LARKMGYLAANLLREPVREIKVALRGPLFTCGDEPVCFEVPYHYSPFTVAGLKGMLEF
                     SHGPEVNYMSAPLLAEDKGIAVEETRADGGTYRNLLELSITSSGGRETVTVCGTVTEE
                     GRQRVVKLDGYPIEFVPEGVVLLFSNHDRPGVIGKVGTLLGEAGSNIANFALGRKNGS
                     GLAVAALQIDNDVPDKVVETFKADADLLWAVKVDFSEAAR"
     gene            5416..6186
                     /locus_tag="PK849_03390"
     CDS             5416..6186
                     /locus_tag="PK849_03390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPE65200.1"
                     /translation="MSCGHDHEKEEINGMKPENSGERSPEVKEAAGTSPLRPTGAAGK
                     RIFLVRHGETSWNREGRFQGQIDVPLNDAGLRQADAVANTLKDVPFDRIISSNLSRAL
                     RTALPLASRMRMGMGVETDDDLQEIGHGLWEGRTAAEVETMYPGALALWHTRPETVVM
                     PEGESLADVQRRAWPALLRIAEGKGETVAVFTHDAVLKVLLMKALDCPLSSFWRFQLA
                     NGSITLLELGNKGWRIPLMGEAGHLGSVFGKRAEQKGL"
     gene            complement(6302..6964)
                     /locus_tag="PK849_03395"
     CDS             complement(6302..6964)
                     /locus_tag="PK849_03395"
                     /inference="COORDINATES: protein motif:HMM:NF016921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HPE65201.1"
                     /translation="MFNQVDDLLEYAQGKTYRTILADPPWQFSNRTGKMAPEHKRLNR
                     YPTMPLGEICSLPVKEIAADTAHLYLWVPNALLPDGLTVLSAWGFEYKTNLIWYKTRK
                     DGGPDRRGVGFYFRNVTEMILFGVKGKNARTLQPGRSQENIITAQKREHSRKPDEQYR
                     LIESCSEGPYLELFARGPRPGWDVWGNQSEEYAPDWATYANHSQAKRDNPQQLTLIET
                     QA"
     gene            complement(6957..7553)
                     /locus_tag="PK849_03400"
     CDS             complement(6957..7553)
                     /locus_tag="PK849_03400"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HPE65202.1"
                     /translation="MPIELIPLNIRENYEVHEWKHAVAILSQDFPDEWTDILAVLESF
                     RLKKSWITDPGGRKSKVSEHIDSFLYRRGWVEKEFQTKVVVDDLSMSSPTHKVDCFKN
                     KVALEIEWNNKDPFYDRDLNNFRLLFDLRAISAGVIITRCDDLQDIFNDLGRGPSYGA
                     STTHMRKLLPKIEGGSGGGCPVLVFGITKHLYEEDDHV"
BASE COUNT         1384 a         2506 c         2398 g         1470 t
ORIGIN      
        1 gctcgcgtcg gcgagcgtga ggacctttcc gtcgccgagg taccccttct tccgggcgat
       61 aacgtagcag ttgagcatga gcagcacgcc gatgatcacc cccggaacga cgcccgcaag
      121 gaagagatcg cccaccgagg ttcccgagac gatgctgtag aggacgaacg gaatgctcgg
      181 cggaatgatg acgccgatgg tcccggccgc ggcggtgagc gcggaggcga agcccgcgtc
      241 gtagttgcgc tctttcatcg cggggatcat gaaggagccg atggcggaga ccgtcgccgg
      301 tcccgagccg gagatggcgg cgaagaacat gcaggcgagc accgtgacca tggcgagccc
      361 cccgatgatg aacccgacga ggctttcggc gagcgccacg agcctccgcg agatgccgcc
      421 gtagctcatc agcgccccgg cgaggatgaa gaacgggatg gcgagcagcg ggaaggagtc
      481 gagcgacgag aacgacttct gggcgatcat gatcagcgag acgttggtgg tgaagtggag
      541 catgatcgcc gtggccaggc ccagcgcgat gccgatgggg atgctggtgg cgatgaggac
      601 gatcagaagc gcgaagagca gcagcgtctc catcagcagg ccgctccctt tcgcaggtcg
      661 cggagcagat ccttcatctc catgacgagg cggatcgcca tcatggcgca cccggcgggc
      721 accgaggcgt acgcccacgc gatgggaatc tgcatggccg ccgacacctg ccagcgcttg
      781 ataagaaacg ccgtaaggac gctcccctgc caggcgatga agagcgcgaa cgcgcaccac
      841 accagcagca cgacgagctc gtaccacttg cggaggcgtc ccttcatgag gtcgacgagc
      901 atctccacgc ggaagtgacg ccgcatccgc accgcgtagc tggcccccac ccacgtctgc
      961 cagagaaaga tgtagcgcgc aagctcctcg ctccacgaaa gcgagttctg gaacacgtac
     1021 cgcatgacca cctggataaa gataaggacc acgttgaagg caagactgcc tacaagaaag
     1081 acttcctcga ctctgtcgaa ggcttttcgc actcttctcc ctcctcttta cagcgcaccg
     1141 caaggggctg ctgctccctt gcggcgcgaa aacaccgatt ctcctgttct gtgaacggat
     1201 gctcccctac tcttcgttgg ctttcttcgc cagctcgacg agctccgcgc cgataacgtc
     1261 cttgaactgg tcgtaggcgg gaagcgtggc gtcgatgaag agctgcttct cttcgggcgt
     1321 cagctcgttg atctggagtc ccgtcttttt gagctcttcg aggaactcct gctcctgcac
     1381 ctccgcgagc gcccgctgct cgacgacgta gcgcttgccc gcgtcctcga cgatcttcca
     1441 gagatcctcg ggcatcgacg cgaagaagtc gtcgttagcc acgagcatcg ttgcggagta
     1501 aacgtgtccg gtgagcgagt agaacttctg cacttcgtag aacttggagg tgtacaccaa
     1561 aacgacgggg ttctcctgtc cgtccaccgt gccctgctgg agcgcggtgt agagttcgcc
     1621 gaagctcatg ggcgtggggt tcgcgccgag catcttgaag aacgcgaggt ggagcgggtt
     1681 ctccatcgtg cgcagcttga ttcccttcag gtcttccggc ttgtggacgg ggccgcggct
     1741 gttggtgatg tgcctgtagc cgttctcgcc gtacccgagg ttgcgcattc caaggggctt
     1801 cagcagctcg tcgaccttat ggccgagttc gccgtcgagc gccttgaacg ccgtttcctt
     1861 cgacttgaag aggaagggga ggtcgaacac ctggaagcgc ttgtcgaaac cggcgagcgc
     1921 cgacgtcgcc gggatggcca cctgcaccgt ccccagctgg acggattcga tgagctctct
     1981 gtcgccgccg agctgcgcgt tggggtagag ttcgacggag atcctgccgc cgctcttctc
     2041 ctcgatatcc ttcttgaaca catcccgcat gatgatgtgg tccgactggt tctccggaag
     2101 aatatacccg accttgatca catactccgg cgccgcgaac gccgcgcccg tcagcacgac
     2161 cgccagcagt accgccaatg ccgctactcc gcacatacgc ttcattacaa cccctccttt
     2221 tgatataccg gctcctactc cggccttccg tatccggaag attttctgcg attgctataa
     2281 tactacagcg cagtagtaag gtatagtctt tttttcgggc tggcgccacc ggtacgtatt
     2341 atcgcaaaaa cctattgaca ttgatagctt tgactgatat aacgtgaaca tcttttttca
     2401 gggagcggat tttcatggcg cgtacagtcc ttttcaacgg catgggtagt agttcctcct
     2461 cctcttccgg ttggtccgga agggctggtc ttcctgtgcc ggcaggggag acgccgcagc
     2521 cgtagcagtt tcgcaccctt ggaattgaat catcggaatt tcgcagggaa tagaggccgg
     2581 aggggacacg cccttccggc cttttcgcat atttaaagga gggatcgtcc atgcaccccc
     2641 atcttttcac gccgggaccg gtcgaggttc cgcattccgt tcgcgctgcg ggcgccgcgc
     2701 ctctcgtcgg gcacagaagc gaagcgtact tcgcgctgct ggaggagatc gtcgcgctgc
     2761 tgaagaagct gttgcgcgtt tcgcagcccg tgattacgct gccgtcgtcg ggcaccggcg
     2821 cgctggaggc ccttgccgcc aacctgctcg accccggcga cgccgtcatc tccttctcgt
     2881 gcggcgcgtt cggcgaccgc ttccgcgaga tcgcgtcgcg gaggggcgcg actattctcc
     2941 ccgtcgacat cccccgcgga cggacggcga cgccttcgga cgtgcggctg gccctcgaac
     3001 agcaccccgg cgcggcggcg atgctcttca cacataacga gacctcgacg ggtgcgctca
     3061 ccgatcttcg cgcctgcacc tcggccctgc cggaggacgg gccgctcgtc ctcgtcgatg
     3121 cggtcagctc gttcggggcc gtcccctgct tcccggagga gcagcgcatc gacggcctcg
     3181 cctcctgctc gcagaagggg ctgctgaccc ctccgggaat cggaatcgtc gccctctccg
     3241 agcgcggatg gaagcgcgcg gcgtcccgtc cgtccccctc ctactacttt gacctcccgc
     3301 tgcaccgccg ttttctggag aaatcgcgcc cgcagaaccc ctatacgccg cccgtcacgt
     3361 tgctctacag cctcgcggag gcgctgcgct tcctcgacgg gatcggcttc gaacgacggt
     3421 tcgccgacgt cgaacggacg gcgcggacct tcgagaccgc gtgcgagtcg atggggctct
     3481 ccctcttcgt gaaggagcag gagctacgct ctcccgccgt caccgccgtg accgtgccgg
     3541 aggggcgagc ggaggagatg aagcgcgcgc tcgccgaact gggcgtcgag gtcgccgagg
     3601 ggcaggggaa ggagccgatg ctccgcgtgg cccactactc gcccggcggc tggccggagc
     3661 tgtgcctcgt cgcggggagc ctctacggcg cggggcgcgc gtgcggactg gaactttcgc
     3721 cggacttttt ggagcccgcg tggaactgct ggaagaaagg ggcgtgcaca caatgaaggt
     3781 gcttgtcacc gatacgatct ccgaggccgg agtcgcgctg ctgcgaaaag agccggactt
     3841 gaaggtggag gtgcggctcg acctctcccg cgaggagctg ctagaggccg tggccgacgc
     3901 ggacgggatg ctcacgcgga gcggtacgcc gctcgaccgc gaagtgctgg acgcggccaa
     3961 aaagctgaag gccgtcgcgc gcgccggtgt gggcgtggac aacatcgaca tcgccgccgc
     4021 aagccggcgc ggagtcgtcg tgatcaacgc ccctacggga aacaccctcg ccgccaccga
     4081 acacaccatg gcgatgctgc tctcgctcgt ccgcaaggtg ccgcaggcgt ggaactccgt
     4141 cgcctcgggc gagtggaagc gcagccgctt catggggatg cagctctcgg ggaagcgcct
     4201 gctgatcgtc gggctggggc gcatcggaac ccaggtcgcc ctccgctgcc gcgccttcgg
     4261 catggagatc atggccttcg acccctacgt gtcgaaggag aaggccgacc gcgccggagt
     4321 acagctcgtc gacgacctca agggaggaat ggccctcgcc gacgtgatca cgctccacgt
     4381 gccgctgacc agggagtcgc gggggatgct cgacgccgcc acgctccgcg cgtgcaagcg
     4441 gggggcctac cttgtgaact gcgcgcgcgg cggcctggtc gacgaggagg cctgcgccga
     4501 ggccgtccgt tcgggaatcc tcgccggaac cgcgttcgac gtcttcgacg gcgagcccgt
     4561 cagagccgac cacccgctgc tggcgtcgga catccggggg aaggtgatcc tcacgccgca
     4621 catcggcgcg aacaccgagg aggcccagtc cgccgtcgcc gtgatcgccg cgtcgaacct
     4681 cgccgccgcg ctgctcggga agccgtacga gcacgccgtc aacctcccct tcagcgaaca
     4741 gctcctctcc gacggcagcc gcgcctacct ctccctcgcc cgcaagatgg ggtacctcgc
     4801 tgcgaacctc ctgcgcgagc ccgtccgcga gatcaaagtc gccctccgcg ggccgctctt
     4861 cacctgcggc gacgaacccg tctgcttcga agtcccctac cactactccc ccttcaccgt
     4921 cgccgggctc aaggggatgc tggagttttc gcacggcccc gaggtgaact atatgtccgc
     4981 gccccttctt gccgaggaca agggcatcgc cgtcgaggag acccgcgccg acggcggtac
     5041 ctaccgcaac ctgctggagc tctccatcac ttcctccgga gggcgcgaga ccgtcaccgt
     5101 ctgcggcacc gtcaccgaag aggggcggca gcgcgtcgtc aagctcgacg gctacccgat
     5161 cgagttcgtc cccgaaggag tggtgctgct cttcagcaac cacgaccgcc ccggcgtcat
     5221 cggcaaggtc ggcaccctgc tcggcgaggc cggatcgaac atcgcgaact tcgccctcgg
     5281 ccggaagaac ggaagcggcc tcgccgtcgc cgcgctccag atcgacaacg acgtccccga
     5341 caaagtcgtc gagacgttca aggccgacgc cgacctgctc tgggccgtca aggtcgactt
     5401 ttcggaggcg gcgcgatgag ctgcgggcat gatcatgaga aggaggagat aaacggtatg
     5461 aagccggaaa attcagggga gcgttccccg gaggtgaaag aggctgcggg gacgagtccc
     5521 ctgcgaccaa cgggagctgc ggggaagcgc atcttcctcg tccggcacgg cgagacgtcg
     5581 tggaaccgcg aagggcgctt ccaggggcag atcgacgtcc cgctcaacga cgccggactg
     5641 cgccaggccg acgccgtcgc caacaccctg aaagacgtcc cattcgaccg gataatctcc
     5701 agcaacctct cccgcgcgct ccgcaccgcc ctccccctcg cctcccgcat gcgcatgggg
     5761 atgggcgtcg aaaccgacga cgaccttcaa gagatcggcc acggcctctg ggagggacgc
     5821 accgccgccg aagtggagac gatgtacccc ggagccctcg ccctctggca cacccgcccc
     5881 gagaccgtcg tcatgcccga aggcgagagc ctcgccgatg tgcaacgccg agcctggccc
     5941 gccctgctgc gcattgccga gggaaagggc gagaccgtcg ccgtcttcac ccacgacgcc
     6001 gtgctcaaag tcctgctgat gaaggcgctc gactgccccc tctccagctt ctggcgcttc
     6061 cagctcgcca acgggagcat caccctgctg gagctgggaa acaaggggtg gcgcattccg
     6121 ctgatgggcg aggcggggca cttgggaagc gtcttcggga agagggcgga gcagaagggg
     6181 ttgtaacaaa tcagaccgat gaacgtcttt tccgacagta ggctccaccg gacccaaacc
     6241 ttcgcctcga atatgagcaa aactccctgc tgcgtctccg aggcgaaggt tcagtattgc
     6301 atcacgcctg tgtttcgatc aaggtgagct gctgcgggtt gtctcgcttg gcctgggagt
     6361 ggttcgcgta ggtcgcccag tcgggggcgt actcctctga ctggtttccc cacacgtccc
     6421 atccggggcg cggaccgcgc gcgaagagtt ccagatacgg cccttcgctg cacgattcga
     6481 tcaggcggta ctgctcgtcc ggctttctgc tgtgctctct tttctgcgcc gtgatgatgt
     6541 tctcctgaga gcgtcccggc tggagggtcc gggcgttctt gcccttcact ccgaagagga
     6601 tcatctcggt gacgttccgg aagtaaaagc cgactccgcg gcggtcgggg cctccgtcct
     6661 tccgcgtttt gtaccatatc agattcgtct tgtattcgaa gccccatgcc gaaagcaccg
     6721 tcagcccgtc gggaaggagc gcgttgggaa cccataagta gagatgcgcc gtatcggcgg
     6781 cgatctcttt caccggaagg ctgcaaatct cgccgagcgg catcgtcgga tagcggttga
     6841 gccgtttatg ttcgggcgcc atcttgccgg tgcggttcga gaactgccag ggaggatcgg
     6901 ccaggattgt cctgtatgtc ttcccctgcg catattccag cagatcgtct acttgattaa
     6961 acatgatcgt cttcctcgta gaggtgtttg gttatgccga acaccagcac aggacatccg
     7021 ccgccgcttc cgccttcgat tttgggtagc agctttctca tgtgggtggt cgacgcgccg
     7081 taggaggggc ctcgtccgag atcgttgaaa atatcctgca ggtcgtcgca tcgggtgatg
     7141 ataactcccg cgctgattgc ccgcaggtcg aaaagcagcc ggaaattgtt caaatcacgg
     7201 tcgtagaagg ggtctttgtt gttccattcg atctccagtg ccactttgtt tttgaaacag
     7261 tcgactttat gcgtgggcga cgacatcgac aagtcgtcca cgaccacttt ggtctggaac
     7321 tccttttcaa cccagccgcg cctgtagaga aagctgtcga tgtgttcaga caccttcgat
     7381 tttcgcccgc ccggatccgt aatccatgat tttttcaaac gaaaggattc caggacggcc
     7441 agaatatccg tccactcgtc ggggaaatcc tgcgaaagga tggcgacggc gtgcttccac
     7501 tcgtgaactt cgtaattttc tctgatgttg agcggtatca actctatcgg catgttttca
     7561 cctcttccgc atcgtaccat attttcacga atttgtggcg cctgtcgtgg gggaaatacg
     7621 tccgttgcgc tctgcgtaca atcagccttt ttctctcaat cgacgttttt cctaaaattt
     7681 tcttcggtgg acgtcctgca tcaccctccg agaatcgcgt cgatgaactc cgcttttttc
     7741 ttcaggccct tgccgggg
//
LOCUS       DAOPKU010000070         7716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10099_length_7716_cov_5.124918, whole genome shotgun sequence.
ACCESSION   DAOPKU010000070 DAOPKU010000000
VERSION     DAOPKU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10099_length_7716_cov_5.124918"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PK849_03405"
     CDS             <1..311
                     /locus_tag="PK849_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65203.1"
                     /translation="AVGGSHSTFGKADIVPPGGGWYTPEDTERVDMGEGVFRAVLLSG
                     VPVLHFLNLRPLARSAGIPFDVPPRSRFGGGAGDAACAAGLLFYVVFLFRFHRWRRDE
                     "
     gene            308..1846
                     /locus_tag="PK849_03410"
     CDS             308..1846
                     /locus_tag="PK849_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPE65204.1"
                     /translation="MICSHDVKQEITRQEEHVFLDGLSQKEKDGEVQSALLPRGSAEK
                     FFFSGLFFLGLWLLAQGLSAQPVLEILGRLPETMDSGDLLSAASMLVLMNSLRAIALY
                     LGWFLLGSGLGALHPSVSFLSWFVPVVAIPVTYFILPSLGEGVQLHFGFPAVLSVSSV
                     LVLRYLTRGIADWGYKAIALALFVFSFQWLDVIPALTSWGAGWGELSSAVKTAALLME
                     REYLLNRAGIAVFGGLFCAGVITTELLVSYSARLYSLALLRDRENKVALLREENLRNR
                     SLVEMQQLVHDLKRPLTTIMGLADVIASGKSAPSAGKYGAVIVEAGRSMEEMISEILH
                     EDFRRPIALGDLLQYVFTQISPFDWRETVSLSAEEGILDEYVTVNVVRLSRALVNLLE
                     NAHRANTLVNGSRIVMRISGTQEFAEIAVLDEGPGFRRTRGEEDGKPLKAGGLSGMEW
                     SSSSGWNSTGLGLQYVTMVVRNLGGKFVIEDLPDGGARTAIVLEKNAAGKESSKAGRE
                     AFGM"
     gene            1876..2520
                     /locus_tag="PK849_03415"
     CDS             1876..2520
                     /locus_tag="PK849_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65205.1"
                     /translation="MPLHIGAIDDDRSILYTLEAMASTEGWRMLTTNVPEECLDWIKL
                     DEVDILLVDYHMPVMNGLHLIRKARELSSKLVLIALTVEDGGDLAGQLVLAGADDFIT
                     KPIRLADFTARIRLHEKLFAHREQLNWEERKKGVSKDTMRKVLEKLKEFSSPKDCDDV
                     AAACGLAYVTAHRYLEYLAERGLIIRSAGVQDGRPGRPKTYYAVPVLSSNAASQ"
     gene            complement(2583..3326)
                     /locus_tag="PK849_03420"
     CDS             complement(2583..3326)
                     /locus_tag="PK849_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE65206.1"
                     /translation="MKKILFALFALLVLGTAGWGNVMEKDALLVGTESTYPPYEFRDA
                     NNQLKGFDIEMMETIAAKIGKKIEWVDMPFDSLIPSLLAKKIDIVAAGMSATEERAKR
                     VAFSTNYEISISAFIVAEGNDSMKVLEDMKGKTIAVQLGTVQETYSQSLEGVVVKSFQ
                     KFDDCVREVILGRVDATLMDTPVAKSYVEHKDFQGKIKIAFEQEITGSGKALAMNLGE
                     TELIEAVNKALAELEGSGELDALKKQWFK"
     gene            3540..4046
                     /locus_tag="PK849_03425"
     CDS             3540..4046
                     /locus_tag="PK849_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPE65207.1"
                     /translation="MTEKKEAFYLIFRVGERRFAIAAAEIVRVLAAVEVTPLPDTPSF
                     LRGVINCGGVLVPVIDLRVRFGLPVRRVRPSDRLIFVRNAASAFQKDGRLCSFLVEDV
                     EGVFPLAPETIALPESSSGLFNAALAGLESGDDRVVCIQTADTLFSMTEAEILQVERA
                     SPGTEDIL"
     gene            4046..5518
                     /locus_tag="PK849_03430"
     CDS             4046..5518
                     /locus_tag="PK849_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPE65208.1"
                     /translation="MTQSSRFILSPEAEQDLRRLGLLVEKRLGLVFSEKRSRDLWQAM
                     LSLAAFAQRSPTELMHKWANEGLSEAATTALAEYLTIGETYFFREPKTLRAFEELVLK
                     RFTSGKKPERFRIWCAGCSTGEEAYTLSIILHRLLPDIRSMDVSILGTDINPRVLEKA
                     RRGAYSSWSFRGLEKSLKERFFEPLDDGSWSVKHLFRQRVSFSLLNLADDEWRLWDDG
                     SHPDVLFCRNVLIYFSADQRKNVVFRFHSLLPPEGWPVVAPCESSALLASKFTPVHHD
                     GATLYKKQAVPEPTERDVSFPEPLFSALPVGAMLDEAPMEEEPAETGVPYEEPPFLAA
                     ETFLGTVPEYQEDEEPGEALSESVGACLQNARILADKGRHDEALQWALKAKDLDRTAP
                     EPLYLAALISSETGDEREAAASLRSALFLAPDFVMAHYALASLAMKSGRDADSERHLR
                     NAEALLSSLPPDAPLREGDGLCAGDLLQTVRGLRRNSIFE"
     gene            5537..6082
                     /locus_tag="PK849_03435"
     CDS             5537..6082
                     /locus_tag="PK849_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPE65209.1"
                     /translation="MEISADALDQERMDAVLRKRAKKLSREFLKESADEEDLLEIVEF
                     RLGQERYGVASLHVGEVIPLRERAVLPCTPPFVAGILNLRGRIVSLIDLKFFFDIPAT
                     EISKETRVIVLRSGEMELGILADALVGIRMISLRNIHPPLPTMAGVRSAYLLGVEGED
                     TALLDGGRILKDESLIVNEEV"
     gene            6101..>7716
                     /locus_tag="PK849_03440"
     CDS             6101..>7716
                     /locus_tag="PK849_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE65210.1"
                     /translation="MVKLRNVSIGKRLGFGFGTVVLIALALGITGILGISRLSNDLEY
                     ITTNRISDLRSLAGLNYQRMAIRAETLGVVITEDKSNRDEELLKILERRKKASDRIDE
                     HWNALLSVPRHMEKGRQLVEILKVQYQSWRKIHGDIDRLVEEILRSQSVDRKNTLFQE
                     LKAQVYRSIPISDVMGSTFDDLTRNNNEQTDLLVEGDLKRAGMMEMFFLLAMIVGVFI
                     ALVMTVKISRSIVLPIASAVSFLSRLREGELRENVPQEFLMRKDEIGTLAQAVQDLTE
                     ELREQIASMKEVTVALASSAYQISASVSEVTAGAEETAVAVVETTATMEEVRTTAEVT
                     NRKSKEVAESAQQGLQVVQKGKSATDALFDGMKNVGERMTSIAETIIRLSEQSQEVGE
                     ITETVEDLAEQSNLLAVNAAVEAAKAGEQGRGFSVVAQEIKSLAEQSKQSAKEVQRIL
                     RDIQKATSAAVMAIEQGSKAVEQGAKDAAPSRESIQGITKKFVESAQSAAQIAAANNE
                     LLAGIDQVTQAMESIKAAGEQNVAGMKDLEA"
BASE COUNT         1632 a         2021 c         2305 g         1758 t
ORIGIN      
        1 ttgcagtcgg aggctcgcac agtaccttcg ggaaagctga tattgtccct cccggcggag
       61 ggtggtacac gcctgaagac acggaaagag tcgacatggg cgaaggggtg ttccgtgctg
      121 ttcttctttc gggcgtaccg gtgcttcact ttttaaacct ccggccgctt gcacgaagcg
      181 cagggattcc tttcgacgtc cctccccgct ctcgtttcgg cggcggggcc ggagatgctg
      241 cgtgcgccgc agggctcctg ttctacgttg tttttctttt ccgctttcac cgttggagga
      301 gagacgaatg atctgttccc atgacgtgaa gcaggaaatc acccggcagg aagagcatgt
      361 gttcttggac ggtctctctc agaaagagaa agatggagaa gtccaaagcg ctcttcttcc
      421 tcgcggaagc gccgaaaaat ttttcttttc cggtcttttt tttctggggt tgtggctgct
      481 ggcgcagggg ctttccgccc aacctgtgct tgagattctc ggcaggcttc ccgagacgat
      541 ggacagcggc gatcttctct cggccgcgtc catgctcgtc ctgatgaact cgctcagggc
      601 catcgcgctc tatctcggct ggttccttct gggaagcggc ctgggagcgc ttcacccctc
      661 ggtctctttt ctctcgtggt tcgtgcctgt tgttgccatt ccggtgacat attttattct
      721 tccgtcgttg ggggaggggg ttcagctcca ttttggcttc cctgccgtgc tgagcgtgag
      781 cagcgtgctt gtcctccgct atctgaccag aggaatcgcc gactgggggt acaaagccat
      841 cgcgctcgcc cttttcgttt tctcttttca gtggctcgac gtcattccgg cgctcacatc
      901 ctggggggcg ggttggggcg agctctcctc cgccgtgaag accgcagcgc ttctgatgga
      961 acgggagtat ctgctgaaca gagcgggaat tgcagtcttc ggaggtcttt tctgcgccgg
     1021 agtcatcaca acggaactcc tcgtcagcta cagcgcccgt ctctacagct tggctcttct
     1081 gcgggaccgg gagaacaaag tggctctttt gcgggaggag aacctccgga accggagcct
     1141 tgttgaaatg cagcagcttg ttcacgatct gaagcgcccc ctgacgacga tcatggggct
     1201 ggccgacgtt atcgcctcgg gaaaaagcgc cccgagcgcg gggaaatacg gcgccgtcat
     1261 tgtcgaggcg ggacgttcga tggaggagat gatctccgag attctccacg aggattttcg
     1321 ccgtcctatt gccctcgggg atcttctgca gtacgtcttc acccagatca gcccgttcga
     1381 ttggcgagag accgtgtcgc tctcagctga agagggaata ttggatgaat acgtgacggt
     1441 gaatgtggtg cgtctttcca gagcgttggt gaatctgctg gagaatgcgc acagagcgaa
     1501 tacactcgta aacgggagca ggattgtgat gcgcatctcc ggaacgcaag agtttgctga
     1561 gatagctgtc ctcgacgaag gtcccggttt tcgaaggacg cggggagaag aagacgggaa
     1621 gcctttgaag gccggaggtt tgtccgggat ggagtggagt tcatcttcag gatggaattc
     1681 cactggtctt ggtctccagt acgttacaat ggtcgttagg aatctcggag gcaagtttgt
     1741 catagaggat ctccccgacg gaggggcgag aacggcgata gtccttgaaa aaaacgcagc
     1801 cggcaaagag tcttcgaagg cggggcgtga agccttcgga atgtaaaaga tcctaatgaa
     1861 aggatgggaa gcgcaatgcc tcttcacatc ggtgcgatcg acgacgacag gtctattctc
     1921 tatacgctcg aagccatggc ctccacggaa gggtggcgca tgctcacaac gaacgttccg
     1981 gaggagtgcc tcgactggat caaactcgac gaggtggata ttctgctcgt cgactatcat
     2041 atgcctgtaa tgaacggtct gcacctgata cgaaaggcga gggagctctc ctcgaagctg
     2101 gtgctgatag cgttgaccgt ggaggacggc ggcgacctcg ccggtcagct cgtgctggcc
     2161 ggagcggacg attttatcac gaagccgatt cggctggcgg attttaccgc ccggatcagg
     2221 ttgcacgaga agctctttgc tcaccgggag cagctgaact gggaagaacg gaaaaaaggc
     2281 gtcagcaagg atacgatgcg gaaggtcctc gagaagctga aggaattcag ctctcccaag
     2341 gactgcgacg atgttgcggc cgcttgcggg ctggcgtatg taacggcgca caggtatctg
     2401 gaatatcttg ccgaacgcgg gttgatcatt cgcagcgccg gggtgcagga cggccgtccg
     2461 ggacgtccca agacatacta cgcagttccc gtcttatctt cgaacgcggc ctctcaataa
     2521 aaaaagcggg gctctccgtg cgtggagagc cccgcttcat tcagtacgaa aaaaactgaa
     2581 gtctacttaa accactgctt tttcagagcg tccagctcgc cgcttccctc aagttcggca
     2641 agggccttgt tcacagcttc gatgagttcc gtttcgccga gattcatggc aagagctttc
     2701 cctgagccgg tgatttcctg ctcgaaggcg atcttgatct ttccctggaa gtccttgtgc
     2761 tcgacgtagc tcttcgcgac gggagtatcc atcaatgttg cgtcgacgcg tcccagaatg
     2821 acttcgcgaa cgcaatcgtc gaacttctgg aaggatttta caacaacgcc ttcaagagac
     2881 tgactgtagg tctcctgtac cgtcccaagc tggacggcga tcgtctttcc cttcatgtct
     2941 tcgaggactt tcatcgaatc gttgccctcg gccacgatga acgcgctgat cgagatctcg
     3001 tagttcgtgg agaacgcgac ccttttcgcc cgctcctcgg tggcgctcat tccggcggcg
     3061 acgatgtcga tcttcttcgc gagaagcgaa ggaatgaggc tgtcgaacgg catatcgacc
     3121 cactcgattt tcttccctat ttttgcggcg atcgtctcca tcatttcgat atcgaagcct
     3181 ttgagctgat tgtttgcatc acggaactca taaggggggt atgtgctctc cgtgccgacg
     3241 agcaaagcgt ccttttccat gacattgccc catccggcgg taccaagaac cagcagcgca
     3301 aacagtgcga acagaatttt cttcatcgtt ttctctcctc caatatgaac agatgttaag
     3361 tactttccca gtgtgtgata aacattgtac ttgcgagcat acacgccacg tttttccctg
     3421 tcaagaccgc tttcctcctt ttcccaaaga ctgctataat ttctccggac aggagttctt
     3481 tctccagtcg cctggaggac gagcaggtat tcggatcaag ggaaggctgg gagaaagaga
     3541 tgacggagaa aaaagaggct ttctatctta ttttcagagt aggagaaaga cggtttgcca
     3601 ttgcggcggc ggagatcgtg cgagtgcttg cggcagtgga agtcacaccc ttgccggata
     3661 ctccgtcgtt ccttcgcggt gtgattaact gcggtggcgt acttgttccc gtgattgatc
     3721 tccgggtgcg tttcgggctt cctgtcagaa gagtgcgccc gagcgaccgt ctcatctttg
     3781 tccggaacgc agcttccgca tttcaaaagg atgggcgcct ttgctcgttt ctcgtggaag
     3841 atgtggaagg agtgttccct ctcgcgcctg aaacgatcgc tctgcccgaa tcctccagcg
     3901 gtcttttcaa tgcggcgctt gccgggctcg aaagcggcga tgaccgcgtc gtgtgcattc
     3961 agacggcaga tactctcttt tctatgaccg aagcggagat tctgcaagtc gagagggcct
     4021 cccccggtac ggaggacatc ctttgatgac gcagtcgagc cgttttattc tttctccgga
     4081 ggcggagcag gatcttcggc gcctcggtct tttggtagaa aagcgtcttg ggctcgtatt
     4141 ttctgaaaaa cgatctcgtg atctctggca ggcaatgctc tcccttgccg ccttcgcaca
     4201 gcggtctccg accgagctca tgcacaagtg ggcgaacgag gggctttcag aggccgccac
     4261 tacggcgctg gctgagtatc ttacgatcgg agagacctat ttctttcggg agccgaaaac
     4321 actgcgcgca tttgaagaac tggttctgaa gcgtttcaca tccgggaaga aaccggagcg
     4381 tttcaggata tggtgcgccg gatgcagtac gggagaagag gcgtatacgc tctctattat
     4441 tttgcaccga ttgcttccgg atattcgctc aatggacgtt tcgatcctgg ggaccgatat
     4501 caatcctcga gtcctcgaaa aggcccggcg gggagcgtac tcttcctggt ccttcagagg
     4561 gcttgaaaag tctctgaaag agaggttttt cgagcctctt gacgacggga gctggtctgt
     4621 aaagcatctc tttcgccaac gagtttcttt ttccctcctc aatctagccg acgacgaatg
     4681 gcgtctctgg gacgacggct cgcaccccga tgttctcttc tgccggaatg tgctgatcta
     4741 tttttccgcc gatcagcgca agaacgtcgt ctttcgtttc cactcgcttc ttccccccga
     4801 aggctggccc gtggttgccc cgtgcgaatc ctcggcttta cttgcgtcga agtttacccc
     4861 tgtgcatcat gacggggcga ccctttacaa aaaacaggct gtcccggagc cgacggaaag
     4921 agacgtctct ttcccggaac ctcttttttc ggctcttcct gtaggagcga tgctcgacga
     4981 ggctccgatg gaggaggaac ccgcagagac gggggttcct tacgaagaac ctccgttcct
     5041 tgccgctgaa acctttcttg gaactgttcc ggagtatcag gaggatgaag aacccgggga
     5101 agcgctttcc gaatccgtcg gcgcatgcct gcaaaacgcc cggattctcg ccgacaaggg
     5161 aagacatgac gaggcgctcc aatgggcgct gaaagccaaa gatctggacc ggacagcccc
     5221 tgaaccgctt tatctggctg ccctgatcag ctccgagacg ggagacgaaa gagaggctgc
     5281 ggcatcgctc cggagcgccc tctttcttgc tcctgatttc gtcatggcgc actatgctct
     5341 cgccagcctt gcgatgaaaa gcggcaggga tgccgattcg gaacgtcatc ttcggaacgc
     5401 ggaggcgctt ctctcttctc ttccgccgga cgctccgctg cgggaaggcg acggactctg
     5461 tgcgggagac ctcctgcaga ccgttcgcgg gctgcggcga aactccattt ttgaatagag
     5521 ggtggtgaga cggagaatgg aaataagcgc cgatgcgctt gaccaagaac ggatggacgc
     5581 cgtcctccgg aaaagagcca aaaagctttc cagggaattc ttgaaggaga gcgcggatga
     5641 agaggatctt ttggaaatcg tcgaattccg tctagggcag gagaggtacg gagtcgcttc
     5701 gctgcacgtc ggtgaagtta tcccgttgcg tgaacgtgcg gttctgccat gcactccccc
     5761 ttttgtggcg ggaatactca atctccgggg acgcatcgtt tctttaattg atctgaagtt
     5821 ttttttcgac atcccggcga cggagatctc aaaagaaacg agggttatag tgctgcgttc
     5881 gggagagatg gagctgggaa ttctggccga cgctttggtc gggatacgca tgatctctct
     5941 ccggaatatc catccgccgc tgccaacgat ggcgggcgtg cgctctgcat atctgctcgg
     6001 agtggaggga gaggacaccg ctctgcttga cggaggaaga atactcaagg atgaatcgct
     6061 gatcgtcaac gaggaagttt aaagcagtga ggaggacgac atggtgaaat tacggaatgt
     6121 aagcattggc aagagactcg gtttcggttt cggcacggtc gtattgattg cgttggccct
     6181 tgggattacc ggtatactgg gtatttcaag gctctcgaac gatcttgaat acataacgac
     6241 gaaccgcatc tccgatttgc gttcgcttgc cgggttgaac tatcagcgga tggccatcag
     6301 agcggagact ctgggcgttg taataaccga agacaaatcg aaccgggatg aagagttact
     6361 gaaaatcctg gaacggcgga aaaaagcctc ggatcggatc gacgagcact ggaacgcact
     6421 tctctcagtg ccgcgacaca tggagaaagg gagacagctt gtagaaattc tgaaggttca
     6481 gtatcagtcc tggcgcaaaa ttcacgggga tatcgacagg cttgtggagg agatcctccg
     6541 aagccagtcg gtcgacagaa agaatacgct ttttcaggag ttaaaggccc aggtataccg
     6601 gtcgattcct atctcggatg tcatgggctc cacttttgat gatctcaccc ggaacaacaa
     6661 cgaacagacc gatcttcttg ttgaggggga tttgaagcgc gcgggaatga tggagatgtt
     6721 tttcctcctg gcgatgatcg tcggcgtatt catcgcgctc gttatgacgg tgaaaatatc
     6781 ccggagtatc gtccttccga tagcttccgc ggtctctttc ctctcccgcc ttcgcgaagg
     6841 ggagttgcgt gaaaacgtcc cgcaggagtt ccttatgagg aaggacgaaa ttggcacgct
     6901 ggcgcaagca gtccaggatt tgacggagga gcttcgggag cagatcgcct ccatgaagga
     6961 agtgacggtt gccctggcgt cttccgcata ccagatttcc gcctcggttt ccgaggtcac
     7021 cgcaggcgcc gaggaaaccg cagtcgcggt cgtggagacc acggcgacca tggaagaggt
     7081 gcgcaccacc gccgaggtga ccaacagaaa gagcaaggaa gtcgccgaga gcgcgcagca
     7141 ggggttgcag gtggtgcaga aggggaaatc ggcgaccgac gctctgttcg atgggatgaa
     7201 aaacgtcgga gagcgcatga cctcgatagc cgagacgatt atccgcctca gtgaacagag
     7261 ccaggaggtt ggggagatca ccgagacggt ggaggacctt gccgaacagt caaatctgct
     7321 tgcggtgaac gcggccgtgg aggccgcaaa ggccggggag caggggcggg ggttctccgt
     7381 cgtggcgcag gaaataaaga gccttgcaga gcagtcgaag cagtccgcga aagaggtgca
     7441 gcgcatcctc cgcgatatcc agaaggccac gagcgccgcg gtgatggcca tcgagcaggg
     7501 atcgaaagcc gtggagcagg gagcgaagga tgcggcgcct tccagagagt cgattcaagg
     7561 gatcacgaaa aaattcgtcg agtccgcgca gtctgctgcg cagatcgccg cggcgaacaa
     7621 cgagctgctg gctggtatcg accaggtgac gcaggcgatg gagagcatca aggccgcggg
     7681 ggagcaaaac gtggcaggaa tgaaggacct ggaagc
//
LOCUS       DAOPKU010000071         7685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10158_length_7685_cov_5.588073, whole genome shotgun sequence.
ACCESSION   DAOPKU010000071 DAOPKU010000000
VERSION     DAOPKU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10158_length_7685_cov_5.588073"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2378
                     /locus_tag="PK849_03445"
     CDS             <1..2378
                     /locus_tag="PK849_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65211.1"
                     /translation="CVRLESEIRAKEEEAAAPASDPLLLCTEYETEIRALEQEGEQLR
                     AALARKNLLERELLASRRSFLRNIEDLCPWWTEEHLMSADISADAIAFARRTAERKEV
                     LERKRGEGEKSLAQWNRLREDRRSEAASLEKEMGVVEKRAKRASERWGLITRLRSVFT
                     ELCSQEDELNSLEEARSTLLAERDRALEQEPIPPGIMVVLISAFLLLVGAGAALQAFL
                     ASDYLWAFGSATLFMASLLAWFAHKDLSKKYATARGQWVKRLEDLDSRLEETTLLVES
                     QKTRVDRLRTQREELGSSLGINAPRFESAMEHLLAEGEGDNSAQERYTFLSERNRQMA
                     TVLARMDAEGEAMEQEVARTVKELETLNEAWSRWLTEHRFDRNLAPRDMEGIVPRILQ
                     LRSEEASLAARAAEGRELDDAVEAIRKRIQELAGRLSPSFEALGEPAPSAADPASIRA
                     FSTLLRRAAEKRGEIAACERDAAMLRQNLEDARAEASETERTLNDLFAAAQTDGEDAF
                     RALAEAWRKRDALLAGRLQERKVLLGLFGSDEALRAAEEEYAFRSPEDVRSETESLRE
                     RAERARSELDAMVDAKGRISSELERMGTDERLGEQLFARKGMERKLDEHLEEWLSNLL
                     AHHFLEVSRARHERERQPEVIRRAGEYLALMTGDRYHLLSEGDEKGFSVVLEEKQQSR
                     ERKDELKWSSGLGDQVYLSMRLALASLWGKNSEPLPLILDDLLVRFDEGRQRGAAMAI
                     CEAARENQVMLFTCQKGTLDVFRDVVAETGESGSDFLSFHRIENGEFVPVA"
     gene            2353..3414
                     /gene="murB"
                     /locus_tag="PK849_03450"
     CDS             2353..3414
                     /gene="murB"
                     /locus_tag="PK849_03450"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPE65212.1"
                     /translation="MASLFPLRNASEAPMDAASFPAVATAAAERIRALDVGTVSFREP
                     LKEHCSWEIGGPADIFVQPSEDAQVLRLLRFVREEGLPLVVIGRGTNLLFADEGFRGV
                     VMKFGRRFSDFSVDGSSSRARGGLWVPRLVKNLVEAGLSGLEHAAGIPGSFGGLVTMN
                     GGSLRHGVGERVVEVRAVSFAGETRAFSREECGFSYRNSFFQRASDSPESRWIVLEAR
                     LELVPAERNDIRREVLGILEERRGKFPLDKPNCGSVFTNDPSVYELAGPPGKIVEAAG
                     LKGLRVGGAEVSHRHANFIVNLGGATASDVLSLIAEIRRRVHDRIGVWLECEVRYVGA
                     DGALVPASTASENLRPEGK"
     gene            3480..6185
                     /locus_tag="PK849_03455"
     CDS             3480..6185
                     /locus_tag="PK849_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65213.1"
                     /translation="MNAVERVRSFLQELWSLCPKLLLLCTLLLAGFSSAWMVTIADKE
                     MRGALLHEARLLAAAINPERVRLLAGDDSDLECESYRRLKTQLSAVRAADPKRRFVYI
                     MGRKRSGEIFIHVDSDPPDSEDYSPPGQIYEEATPVFAEVLERGSESVEGPLEDRWGV
                     WVSALVPLRDSADTVTALLGMDVDASDWSLNVLRRAALPVALTFAFFSSLLAAVVALR
                     TGRRLKQETARADELAVHARAASVAKSEFLANMSHEIRTPMNGVVGMTGLLLDTDLTE
                     EQRRYAEIVRVSAESLLSLINDILDFSKIEAGKLDLEILNFDLSSLMDDFILTLAVSA
                     EEKGIELLCGVDPDVPVLLRGDPGRLRQILTNFVSNAVKFTHEGEVETRVSLVEESDR
                     DALLRFSVRDTGIGIAEEKMGLLFTKFSQADASTTRKYGGTGLGLAISKQLAEMMGGS
                     VGAASVEGKGSEFWCTARLEKQPENMRRELRPATDLRNVRVLIVDDNATNRRLLTARL
                     TSWGMRPESVSGGSAALASLDAALREGDPFRLAVVDMQMPEMDGETFGSLVRADGRFG
                     ALRMLMLTSLGIRGDARKFAEAGFSGYLNKPVRQHDLRDVLSFILTGDADAPGAIVTR
                     HTVRDALSGFDGSGARILLAEDNITNQHVALGILKKFGLRADAVADGEEALAALSAIP
                     YDLVLMDCQMPRMDGYEAARRVRMMEGAVRDVPIIAMTASAMAGDREKCLEAGMNDYV
                     PKPVSPGVLAGALKKWLPYRGERGGAREASSKESAANGSPVLQSKEQNAESRPWNREA
                     FLERLRGDHELAAEIVGDYLSEIPKEIDALREALATGDAEAGERCAHSVKGVSASVGG
                     ERVRDVAFRMERAAAAGDLEMARASLPRLEEEFELLKDAMKRSGH"
     gene            6299..>7685
                     /locus_tag="PK849_03460"
     CDS             6299..>7685
                     /locus_tag="PK849_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE65214.1"
                     /translation="MRKGIAAVLGIALLLSLSGAAALAASREVVIGMPGDVHSMDPYT
                     LNETINNAINNHLFDTLVETDNNLQPKPGLAESWEVSEDARTWTFHLRKGVTFHNGNP
                     FTVDDVIYSFDRSKDREVGASFVHVLSTVESYEKIDDHTLKVTCKSPNALLLAHFKDL
                     LILNKAHCEGQGNDWIALNPNGTGRYKLVEHVRGDRLVLERNPSFWGEQPIVEKVTFK
                     PITNEGTRTANMLSGAVDLVVNVPVRDVKMLEKNKNVTVIAQPGLRAIYLNMAGWSDT
                     PSKDAKMPLKSPDGSNPFKDIRVREAMYRAINIDEIVEKIMNGFAKPMATYSPEGYNG
                     FNPDVKRLAYDPALAEKLLDEAGYKRQSDGYRFEVTLDAPNDRYINDGPVATAIAGYF
                     EKVGIKVNPNLMSRSVFFSYISTTNKAGDNTHLCMTGWSDSGGESALIGLDMLYSITQ
                     DGEVKPGYGGVN"
BASE COUNT         1450 a         2369 c         2585 g         1281 t
ORIGIN      
        1 cctgtgtccg ccttgagtcc gagattcgcg cgaaggagga ggaggccgcg gcgccggctt
       61 ccgacccgct gcttctctgc acggagtacg aaacggaaat ccgcgcgctg gagcaggagg
      121 gggagcagct gcgcgccgcg cttgcacgca agaatctgct ggagcgcgaa ctgctggcct
      181 cgcgcaggag cttcctccgc aatatcgagg atctctgtcc gtggtggacg gaggagcacc
      241 tgatgagcgc ggacatctcc gcggatgcga tcgccttcgc ccgcaggact gcggagcgca
      301 aggaggtgct cgaacggaag cggggcgagg gagagaagtc gctcgcccag tggaaccgcc
      361 ttcgcgagga ccgcagaagc gaggctgcct cgctggaaaa ggagatgggc gtcgtcgaga
      421 agcgggcgaa gcgggcctcc gaacgctggg ggctcatcac gcgcctccgg agcgtcttta
      481 ccgagctctg cagccaggag gacgaactga actcccttga ggaggcgcgt tcgacgctct
      541 tggcggagcg ggaccgcgcc ctcgaacagg agccgattcc gccgggaatc atggtcgtcc
      601 tgatctccgc attcctcctg ctggtcggcg cgggcgccgc gctccaggcc ttcctggcgt
      661 cggactacct gtgggccttc ggctcggcga cgctcttcat ggcctcgctg ctggcgtggt
      721 tcgcgcacaa ggatctgtcg aagaagtacg cgactgccag gggtcagtgg gtgaaacggc
      781 tggaggatct cgactccagg ctcgaggaga cgaccttgct cgtcgagagc cagaagacga
      841 gggtcgatcg cctgcggacg cagagggaag agctggggag cagtctcggc atcaacgcgc
      901 cccgcttcga gagcgcgatg gagcacctcc tcgccgaggg cgagggggac aacagcgcgc
      961 aggagcgcta caccttcctc agcgaacgga acagacagat ggcgaccgtt cttgcgcgca
     1021 tggacgccga aggcgaggcc atggaacagg aggtcgcgcg taccgtcaag gagctggaga
     1081 cgctgaacga ggcgtggagc cgctggctga ccgaacacag gttcgaccgt aaccttgctc
     1141 cccgcgacat ggaggggatc gtcccgagga ttcttcagct gcgctcggag gaggcctctc
     1201 ttgccgcgcg ggcggccgag gggagggagc tggacgacgc cgtcgaagcc atccggaaac
     1261 ggattcagga gctcgcagga cgtctctcgc cctccttcga ggcccttggc gaacctgcgc
     1321 cctcggcggc agatcccgcg tcgatccgcg ccttctcgac gcttttgcgg cgggcggcag
     1381 aaaagcgcgg cgagatcgcc gcgtgcgagc gggacgccgc gatgctccgc cagaatctgg
     1441 aagacgcgag agcggaggcg agcgagacgg agcgcacgct gaacgacctc ttcgcggcgg
     1501 cgcagaccga cggcgaggat gcgttccgcg ccctcgccga ggcgtggcgg aagcgcgacg
     1561 cgctgctcgc cgggcggctc caggagcgga aggtgctcct gggtctcttt ggttcggacg
     1621 aggcgcttcg ggctgccgag gaggagtatg cgttccgttc tccggaagat gtcaggtcgg
     1681 agacggagtc gctccgcgaa cgcgcggaac gcgcccgctc cgaactcgac gccatggtcg
     1741 acgccaaggg gcgcatctcc tccgagctgg agaggatggg gaccgacgaa cggctgggtg
     1801 aacagctctt cgccagaaaa gggatggagc ggaagctcga cgagcacctt gaagagtggc
     1861 tctcgaacct cctggcgcat cacttcctgg aagtctccag agcgcgtcac gagcgcgaac
     1921 ggcagccgga ggtcatacgc cgggcgggcg agtacctcgc cctgatgacc ggcgaccgct
     1981 accatctgct ctccgaagga gatgagaagg ggttctccgt ggtgctggag gagaaacagc
     2041 agtcccgcga gcggaaggat gagctcaagt ggagttccgg cctgggcgac caggtctacc
     2101 tttcgatgcg gcttgccctc gcctctctgt gggggaagaa ctccgagcca ttgccgctga
     2161 ttctcgacga ccttctggtg cgcttcgacg aggggcgtca gcgcggcgcg gcgatggcga
     2221 tctgcgaggc cgcccgcgag aaccaggtga tgctcttcac ctgtcagaag gggacgctcg
     2281 acgtcttccg cgacgtcgtc gccgagacgg gagagagcgg gtcggatttc ctctccttcc
     2341 accgcatcga gaatggcgag tttgttcccg ttgcgtaacg cctccgaagc gccaatggac
     2401 gcggcttcgt tcccggccgt cgcaacggcg gcggccgaga ggatccgcgc gctcgacgtg
     2461 ggaacggtct ccttccggga gccgctgaag gagcactgct cgtgggaaat cggcggcccg
     2521 gcggatattt tcgtccagcc ttcggaggac gcgcaggtgc tccgcctcct ccgctttgtc
     2581 cgggaggagg ggctccccct cgtcgtgatc ggcaggggaa cgaatcttct cttcgccgat
     2641 gaagggttcc ggggcgtggt catgaagttc ggacggcgct tctccgactt ttccgtcgac
     2701 ggttcgtcga gccgtgcccg cggaggcctc tgggttccgc gtctggtgaa aaatcttgtc
     2761 gaagccggac tctcgggcct tgagcacgcg gcggggatcc ccgggtcgtt cgggggcctc
     2821 gtcacgatga acggcggcag tctccggcac ggggtcggcg aacgcgtcgt cgaggttcgg
     2881 gcggtctcct tcgcggggga gacgcgcgcc ttttcgcgcg aggagtgcgg attctcctac
     2941 agaaactcgt tttttcagcg cgcctcggac tccccggaga gccggtggat tgtccttgaa
     3001 gcccgtctcg aactcgtccc ggcggagcgg aacgatatcc gcagggaggt gctgggcatc
     3061 ctcgaggagc ggcgcgggaa gtttccgctc gacaagccga actgcggctc cgtcttcacg
     3121 aacgatccgt ccgtctacga acttgcggga cctccgggga agatcgtcga agcggccgga
     3181 ttgaaagggc tgcgggtcgg cggcgcggag gtctcgcacc ggcacgccaa cttcatcgtc
     3241 aacctcggcg gcgcgaccgc gtcggacgtg ctctccctca tcgcggagat tcgcaggcgc
     3301 gttcacgacc gtatcggagt atggctcgaa tgtgaagtcc gttatgtcgg cgccgacgga
     3361 gccctggttc ccgcgtcgac agcctcggaa aatctgcggc ccgaaggaaa gtgaaacgac
     3421 ccccggcgag cgaaactgcg cttccggggc gccggtttcc ggaaacgggg gggaaaccaa
     3481 tgaacgcagt tgaacgcgtg cgcagcttct tgcaggagct ttggtcgctc tgtccgaagc
     3541 tgctgctctt gtgtacgctc ctcctcgcag gcttctcctc ggcatggatg gtgaccatcg
     3601 ccgacaagga gatgcgaggc gctctcctgc atgaagcgcg cctcctcgcc gcggcgatca
     3661 accccgaacg agtgcggttg ctcgcgggag acgattccga cctggaatgc gagtcgtacc
     3721 gccggttgaa aacacagtta tccgccgttc gcgcggccga ccccaagcgc cggttcgtct
     3781 acatcatggg aaggaaacga agcggagaga tcttcatcca tgtcgacagc gacccgcccg
     3841 attcagagga ctactcccct cccggccaga tatacgagga ggcgacaccg gtatttgccg
     3901 aggtcctgga gcgcgggagc gaatccgtcg aggggccgct ggaggacaga tggggcgtct
     3961 gggtctccgc gctggttcct ttgcgggact ctgccgatac tgtaaccgcc cttctgggaa
     4021 tggatgtcga cgcctccgac tggagcctga acgtcctcag gcgcgccgcg ctcccggtgg
     4081 cgctgacttt cgcgttcttc tcatcgcttc ttgccgccgt cgtcgccttg cgaacaggcc
     4141 gccgcctcaa gcaggagact gcccgcgccg acgaactggc cgtccacgca agagccgcca
     4201 gcgttgccaa gagcgaattc ctcgcaaaca tgagccacga gatccgcacg cccatgaacg
     4261 gcgtcgtcgg gatgaccggt ttgctgctcg atacggacct gaccgaggag cagcggcggt
     4321 atgccgagat cgtcagggtc agcgcggaat ctctcctctc tctcatcaac gatattctgg
     4381 acttctccaa gatagaggcc ggaaagctcg acctcgagat cctcaacttc gatctgtcca
     4441 gcctcatgga cgactttatc ctgacgctgg cggtgagcgc cgaggagaag gggatcgagc
     4501 tgctgtgcgg cgtcgatccg gacgttcccg tgctgcttcg cggcgatccg gggcgtctgc
     4561 ggcagatcct caccaacttc gtgagcaacg cggtgaagtt cacgcacgaa ggggaagtgg
     4621 agacgagagt ctctctggtg gaagagagcg accgcgacgc gttgctccgc ttctcggtgc
     4681 gcgatacggg gatagggatc gccgaggaga agatgggatt gctcttcacg aagttcagtc
     4741 aggcggacgc ctcgacgacg cgtaagtacg gcggcacggg gctggggctt gccatctcga
     4801 agcagcttgc ggagatgatg ggcggctcgg tcggcgcggc gagcgtcgag gggaagggct
     4861 cggaattctg gtgtaccgcc cgcctggaaa aacagcctga gaacatgcgg cgcgaactcc
     4921 gtcccgcaac cgacctgagg aacgtgcgcg tcctgatcgt ggacgacaac gcgaccaaca
     4981 gacggctttt gaccgctcgg ctgacgtcgt gggggatgcg ccccgagagc gtctccggcg
     5041 gaagcgccgc gctcgcgtcg ctcgacgccg cgctgcggga gggcgatccg ttccgccttg
     5101 ccgtggtgga tatgcagatg ccggagatgg acggcgagac gttcggaagt cttgtgcgtg
     5161 cggacggacg tttcggcgcg ctccgtatgc tgatgctcac gtcgctcgga atacgcgggg
     5221 acgccaggaa gttcgccgag gcgggcttct ccggctacct gaacaagcct gtccgtcagc
     5281 acgaccttcg ggatgtgctt tctttcatcc tgaccggaga tgcggacgct cccggcgcca
     5341 tcgttacccg ccacacggtc cgcgacgccc tttcaggctt cgacggcagc ggcgcgcgca
     5401 tcctcctcgc cgaggacaac atcaccaacc agcacgtcgc cctcggcata ctcaagaagt
     5461 ttggcctgag agcggacgcg gtcgcggacg gagaagaggc gcttgccgcc ctctccgcca
     5521 tcccgtacga cctcgtcctg atggattgcc agatgccgcg catggacggg tacgaagccg
     5581 cgcggagagt ccgcatgatg gagggggcgg ttcgtgacgt gccgatcatc gccatgacgg
     5641 cgagcgccat ggcgggggac agggagaagt gcctggaggc cggaatgaac gactacgttc
     5701 ccaagcccgt ttcgccgggc gtccttgccg gagcgctgaa gaaatggctg ccgtaccgcg
     5761 gtgaacgcgg cggcgcccgg gaggcttcgt caaaagagag cgcggcgaac ggctctcccg
     5821 tactgcaaag caaggagcag aatgccgagt cgcgtccgtg gaacagggag gccttcctgg
     5881 aacgcctgcg aggagaccac gagctggcgg cggagatcgt gggcgattat ctctccgaga
     5941 ttccgaagga gatcgacgcg ctccgggagg ccctcgcaac gggagatgcg gaagccggag
     6001 agcgctgtgc gcacagcgtg aaaggcgtct ccgccagcgt cggcggcgaa agagtccgcg
     6061 atgtagcctt ccgcatggag agggccgcgg ctgcgggcga cctggagatg gcaagagcgt
     6121 ctttgcctcg cctggaagaa gaattcgaac tgctgaaaga cgcgatgaaa cgatcgggac
     6181 actgaaactg tcgaaaaagt gcgattgact ttgccttcgg agcactataa aatgtatccg
     6241 caaaggtata cttagccttg cattcggaat gtcaccacag aaaagggagg aatgtatcgt
     6301 gaggaaagga atcgccgcag tactcggaat cgccctgctg ctctcgcttt cgggagcggc
     6361 cgcgctcgca gcgtcacggg aggtcgtgat cggcatgccg ggggacgttc actcgatgga
     6421 cccctacacg ctgaacgaaa cgatcaacaa cgccatcaac aatcatctct tcgacacgct
     6481 ggtcgagacg gacaacaatc tccagcccaa gcccggcctc gccgagagct gggaagtctc
     6541 tgaggacgcc cgcacctgga cctttcacct cagaaaggga gttaccttcc ataacggcaa
     6601 ccccttcacc gtcgacgacg tcatctactc cttcgaccgt tccaaggaca gagaggtcgg
     6661 cgcttccttc gtgcacgtgc tctccactgt ggagagctac gagaagatcg acgaccatac
     6721 gctgaaagtc acctgcaaat cgcccaacgc tttgctgctg gcgcacttca aggatctgct
     6781 gatcctgaac aaggcgcact gcgaaggcca ggggaacgac tggatcgcgt tgaaccccaa
     6841 cggcaccggc cgctacaagc tggtggagca cgtgcgcggc gacaggctcg tgctggagcg
     6901 caacccctcc ttctggggag agcagccgat cgtcgaaaag gtgacgttca agccgatcac
     6961 caacgaggga accaggaccg ccaacatgct ttcgggcgcg gtcgacctgg tcgtcaacgt
     7021 ccccgtgcgc gacgtgaaga tgctggagaa gaacaagaac gtcaccgtca tcgcccagcc
     7081 ggggctgcgc gccatctacc tcaatatggc ggggtggagc gacacgccgt cgaaggatgc
     7141 gaagatgccg ctgaagtccc ccgacgggag caatccgttc aaggatatcc gcgtccgcga
     7201 ggcgatgtac cgcgccatca acatcgacga gatcgtcgag aagatcatga acggcttcgc
     7261 gaagccgatg gccacctact cgcccgaagg gtacaacgga ttcaatccgg atgtgaagcg
     7321 ccttgcgtac gaccccgcgc tcgcggagaa gctgctcgac gaggccggat acaagcggca
     7381 gagcgacggc taccgttttg aagtcaccct cgacgccccc aacgaccgct acatcaacga
     7441 cggtcccgtg gccacggcga tcgcgggcta cttcgagaag gtcggcatca aggtcaaccc
     7501 gaacctcatg tcccgctcgg tcttcttctc ctacatctcc accaccaaca aggcggggga
     7561 caacacgcac ctctgcatga ccggctggtc cgattcgggc ggcgagagcg cgctcatcgg
     7621 gctcgacatg ctctacagca tcacccagga cggagaggtc aaaccggggt acggcggcgt
     7681 caaca
//
LOCUS       DAOPKU010000072         7683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10161_length_7683_cov_4.975616, whole genome shotgun sequence.
ACCESSION   DAOPKU010000072 DAOPKU010000000
VERSION     DAOPKU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10161_length_7683_cov_4.975616"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PK849_03465"
     CDS             complement(<1..935)
                     /locus_tag="PK849_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPE65215.1"
                     /translation="MAGFVRELTDRMSAIEYAAASLSFPWQGNESSRDLDAAEGLTLA
                     RDILAPEDFPPFDRSTRDGYALISEDSYGASQSNPAFLTIRGEVPMAAVPSFSLSEGE
                     CALIHTGGVLPAGADAVIMLENVERTGNWLEIRKAVQKGENIVFRGEEFSAGAILLPK
                     GVQVDFKSSGVLAAAGISKAPVQHVKVGILSTGDEIVPCETRSLNPGQFRDANANILS
                     SLLRRHGYEPVYLGIVEDSKVSLEKALYRAMEECDVVLLSGGSSVSTRDHCSTILESL
                     EAPGLLVRGILMSPGKPTLIVGIKEKQKLVMGLPG"
     gene            complement(1021..1662)
                     /locus_tag="PK849_03470"
     CDS             complement(1021..1662)
                     /locus_tag="PK849_03470"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPE65216.1"
                     /translation="MSIRESRRHKERQSVIPFGDIMLPVIGLVAVGLLIVGVKLFFLS
                     GDRYKTYTPLPIHQEEKTTPNPSVTPPVLPPGQPEKENAAEDAILAVPVSPGGGTGDR
                     VRVETPTKTPPVVKPKTPPRPPEVKRPAPPPAPSNSRWGVQIGSFTARQSAETVRQQA
                     ERAGFRVSILSGQVRGKTYFRVTVPAGNTRAEAVSLSQKLAKSGFPVFVVSIR"
     gene            complement(1933..3090)
                     /gene="ftsZ"
                     /locus_tag="PK849_03475"
     CDS             complement(1933..3090)
                     /gene="ftsZ"
                     /locus_tag="PK849_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPE65217.1"
                     /translation="MEHVFQVNDTPRPHREEIKVVGVGGGGGNALNHIIRSGVSGVHF
                     IAANTDVGALHLSQASSKVTLGEKLTKGLGAGSDPSVGMNSAKESIDKIREVVAGADM
                     VFITAGMGGGTGTGASPVIAEVAKEAGALVVAVVTFPFSFEMNRRRKQAAEGIATLKE
                     KVDALLVVENDRLLELTDEKTKHSEAYKLADEVLRQAVQGVTDLILKPSLINVDFADV
                     RTVMQNAGSAIMGIGSGEGENRAEIAAKSAIKSPLMSIPMKGAKGILFNITGGSDLGI
                     HEIRKAAQIITETADEDAIVIWGHTVAEDMAEKIQITVIATGFSSEKEKRTVTNFAAK
                     QPAPKQKAPGVVLEEAEVLPIQDEDMFSISGVPKNPYDIPSIIRRNKKAGK"
     gene            complement(3123..4436)
                     /gene="ftsA"
                     /locus_tag="PK849_03480"
     CDS             complement(3123..4436)
                     /gene="ftsA"
                     /locus_tag="PK849_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPE65218.1"
                     /translation="MYKEPEILVGLDLGTSKISVVIAERDSRFQEAQIIGVGYAPSQG
                     IRKGLIINLEQAVRSVRQAIKDAENMVGFEIDEATVSFSGMEVASVTSRGMISLGRTP
                     RQVSISDVERVIEAAQSELSIPPNRVTLHTIPVKYSIDGNSGIDDPLGMTGIRLEMEL
                     QSVIVPTTVVQNIINCVESAGIEVNGLVIKSLASALGSLTDEETRAGVISLCIGGGTT
                     GVALYTDGRPVKLSIIPIGGDHITNDLAYVMKIPISKAEEVKKMVSLNPEEIEQQDLD
                     VDVRGRMKTFSIGEAAEVVNCRLEELFGEHVRHQLAEFSLHMFPAGIVLSGGVAKTPG
                     IDVFVSETLNLPVRIAEPLDVYQMPPGRNDCEYTTVAGVVRYMTSRERNPYRYIETPI
                     SELRSGAAQVASSITGKLPRKSGGDRTEGIRGFMDYLKKSVKELF"
     gene            complement(4437..5231)
                     /locus_tag="PK849_03485"
     CDS             complement(4437..5231)
                     /locus_tag="PK849_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65219.1"
                     /translation="MGKWRGLFFLFAFFMGMLFGGEDFFQPFSLRNIRIESEDPVAEL
                     FFWKNISSRALRFWPAFFLEKDDLRKAVEIQAPLFCTISRRGITGVEVQIEPLRPWIV
                     AQWNKREYYVSQDGFAWDTANELNKMIRGIVRPNAPLITFSDDFPSPALENSSAIVSR
                     VVFPLELLQGWLDGLAENRWGARVEQISVSRREGKFLLKLVFDKGGAHVLLWGDRPRW
                     KELSSALSQIMEQLHFLGNNIIIDTTYTDRIIVRGMARGDQEGSGR"
     gene            complement(5324..6745)
                     /gene="murC"
                     /locus_tag="PK849_03490"
     CDS             complement(5324..6745)
                     /gene="murC"
                     /locus_tag="PK849_03490"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPE65220.1"
                     /translation="MDTTISNFDGIHRIHLMGIGGAGMSGLALLLAELGFEVSGCDMI
                     HTSYIDKVLKEEVAVVLGHGHAHMEKFLPDLLIYSSAIPEDNEEILAARARGIAVAKR
                     GEVLSWIFDSRCGIGVAGTHGKTTTSSMISLILEQAGFHPTLAIGGELCDIGVNAKLG
                     EGRFMVAELDESDGSFEKFHPEISVITNVDWDHVNFYPTFESVLEAFSRFFSNLKSDG
                     LLVFCGEDAGLARLLSLGRIPEGAISYGWGASWNWGASNVYHKPGGGVSFTLLHEGQR
                     VGEVDLAISGEHNILNTLASCAVAHRLGIPFPVIKRTLRDFKGAKRRLQHVGSFEGID
                     VYDDYGHHPREIAATLGTLEHMFPERPLRVVFQPHRYTRTQALFKDFASVLAVADTLI
                     LLPIYQADEEPISGITSLLISDHLPAEKKQACLMPKTLDDAVFLLEDLCEEGDIVLTI
                     GAGDVSLIGDNLLAKAKTHLVRV"
     gene            complement(6746..>7683)
                     /locus_tag="PK849_03495"
     CDS             complement(6746..>7683)
                     /locus_tag="PK849_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPE65221.1"
                     /translation="SHITPYYLPTSGSPLGIRSLSGKMRRLKEFGLSFISFKKLLRNC
                     HVDACVLFGGYVSFIPLMVCHLTGVPVFVHEQNSVAGKVTRLSSRLGKKVASGWEVCT
                     PLSPDAFHWTGVPVRGFSLREPKEAWELMALGKKLPEKKIIGVLGGSLMSQRLIQLMG
                     PMLRAQEMRNFFFLFLGDSQLADIAREFHGSPENISVIGKQWDMSNFYSVVDAVVTRG
                     GASTLSELATLGIPAVVVPWQEAADNHQERNALCFIEHNDGAIWHENDSPKAFQECIL
                     KILRNTQRPPRSISKGDESESLWRLISSSIGREIL"
BASE COUNT         1969 a         2021 c         1731 g         1962 t
ORIGIN      
        1 tgtcccggca ggcccattac gagcttttgc ttttctttta ttccgacgat gagagtgggt
       61 ttccccggcg acatcaaaat gcctcgaacc agcagtcccg gtgcttcgag ggattcaaga
      121 atcgttgaac agtggtctct agtgctcact gaagaccccc cgctgagcag gacaacgtcg
      181 cactcctcca tggctcggta taacgctttt tcaagagaaa cctttgagtc ttcgactatt
      241 cccaaataga caggttcata tccatgacgc cgtaaaagcg acgaaagaat gtttgcgttg
      301 gcgtcgcgaa actggcctgg gtttaaagaa cgagtttcac aggggactat ttcgtctccc
      361 gtgctgagaa tacctacctt tacgtgttgc accggcgcct ttgaaatacc tgcggctgca
      421 agaacgccgg aagacttgaa gtcaacctgg acgccctttg ggagaaggat ggcgcctgct
      481 gagaattctt caccccgaaa gacgatattc tcgcccttct gaacagcctt tcgtatttcc
      541 agccaatttc ctgttcgctc tacgttttct agcataataa ccgcatccgc tcccgcaggc
      601 agtacgcctc cagtgtgaat cagcgcacat tccccttcgc tcaaggaaaa cgaaggaacg
      661 gcggccattg gaacttctcc gcggatcgtt aaaaatgcgg gatttgattg cgatgctccg
      721 tagctgtcct cactgatgag cgcatacccg tcgcgggtgc ttctgtcaaa aggagggaaa
      781 tcttccggtg caagaatatc tcgagcaaga gtcaaaccct ccgctgcatc gagatctctt
      841 gaactctcat ttccttgcca aggaaatgag agtgatgcgg cggcgtactc aatggcggac
      901 attctgtcag tcagttcccg aacaaaacca gccaatagcg tatctccttc ccgatccgtg
      961 tgctctccaa aatttgttga agcctacatg aagtggataa ttacaaagca aaaaagcgct
     1021 ttatcgaatc gaaaccacaa aaacaggaaa accagatttt gcaagcttct gcgagagaga
     1081 taccgcttcg gcacgagtat ttccagccgg aacggtcacc ctgaaatatg tcttcccgcg
     1141 tacctgcccg gacaaaatcg agactctgaa gcccgctctt tcagcctgtt gacgtaccgt
     1201 ctcggcgctc tggcgtgctg taaatgaacc gatttgaacg ccccaacgac tgttggaagg
     1261 cgctggcgga ggcgcaggtc gtttgacttc cggtgggcgc gggggtgtct taggttttac
     1321 tactggagga gttttcgtag gagtttctac cctgacacgg tctcctgtgc cacctccagg
     1381 tgaaacggga actgcgagga tggcgtcctc cgcagcgttt tccttctcgg gttgtccggg
     1441 cgggagaaca ggaggtgtga cggaaggatt gggcgttgtt ttttcctcct gatgaatagg
     1501 taacggtgta tacgttttgt acctgtcgcc ggaaagaaaa aagagtttta cacccacaat
     1561 caggaggccg accgccacaa gtccgatgac gggaagcata atatcgccga aagggataac
     1621 agattgcctt tctttgtgtc ttctgctttc tcttatggac atttttacct ccctgtccgt
     1681 ctgcctcgct tggagaagaa gtgccccaaa tggcttccta aatcagcaaa aatttgtctt
     1741 ctgcattaac cccagtttgt agcgcaacgt atttactata atagtgcaca caagcctatc
     1801 taatagatca gaatcctaaa aaataaagca ggaatccctt tcaagacccc tgcttccttc
     1861 agcctgcgct agacaattcc ggatcaatca aattcaactc gctcgaggaa atgattttcg
     1921 ggatatcaga atttacttgc cggctttttt atttcttcga attatcgaag gaatatcgta
     1981 agggttcttg ggcacaccac tgatggaaaa catatcctca tcctgtatcg gcagtacttc
     2041 agcttcttca agaacgactc cgggcgcctt ttgttttggc gcaggctgct ttgctgcaaa
     2101 attcgtgaca gtacgcttct ctttttcgga gctgaagcca gtagcgataa ctgtaatctg
     2161 tattttttcc gccatatcct ccgcgactgt atgcccccaa atgacgatgg cgtcctcatc
     2221 cgctgtctcc gtaattatct gagccgcctt gcggatttcg tgaataccaa ggtccgagcc
     2281 tccggtaatg ttgaataaaa tacccttggc gcctttcatc ggaatggaca tcagaggcga
     2341 tttgatagcg ctcttcgctg cgatttccgc tcgattttca ccttcgcctg agccaatgcc
     2401 cataatagct gaaccggcat tttgcattac agttcgtacg tctgcaaaat cgacattgat
     2461 aagactcggt ttcaaaataa gatcagtcac tccctgaaca gcctgacgga gtacttcatc
     2521 tgcaagttta taggcttctg aatgcttggt tttttcatca gtcagttcaa gaagcctgtc
     2581 gttttcgact acaaggagcg catccacctt ctctttgagc gttgcgatcc cttcggctgc
     2641 ttgttttcgg cgcctgttca tctcgaagga aaagggaaat gtgacgactg cgacaacaag
     2701 cgctcccgct tccttggcga cttcggcaat gaccggagaa gcgcctgttc ctgtacctcc
     2761 gcccatcccc gctgtaataa agaccatgtc ggctccggca acaacttccc gaatcttatc
     2821 aatggattct ttcgcgctgt tcatacccac tgacgggtcg gagccggcgc ccagcccctt
     2881 ggttagtttt tctccaagag tgaccttaga acttgcctgg gaaaggtgca atgcaccgac
     2941 atccgtattc gcagcgatga agtgaacccc ggaaacaccg cttcgaatta tatgattcaa
     3001 ggcgttgccg ccgccgcccc ccacgccgac tactttgatc tcttctctgt ggggcctcgg
     3061 agtatcatta acctgaaata cgtgctccat attcatttcc tccccgaatg cggaatgcgg
     3121 cttcaaaaaa gctctttaac agacttcttt aaataatcca taaaaccacg aatgccttct
     3181 gtccggtctc ctccgctctt tcgagggagc ttccccgtaa tgctggaagc aacctgggca
     3241 gctcctgagc gaagttctga aataggcgtt tcgatgtatc tgtaaggatt gcgttccctg
     3301 ctcgtcatat atcgtaccac accggccaca gttgtatatt cgcagtcgtt tcttcccggc
     3361 ggcatctggt acacgtcaag cggttcggca atccgaacgg gcaagttaag tgtttcggag
     3421 acgaagacat caatgccggg tgtttttgct actcctcctg aaaggacgat gcctgcagga
     3481 aacatgtgga gactgaactc ggcgagctga tggcgaacgt gttcaccaaa aagctcttcc
     3541 aatctgcagt tcactacctc ggcggcttca ccaatgctga aagtcttcat tcgtccgcga
     3601 acgtcgacgt caagatcctg ttgctctatt tcctccggat tgagagagac catcttttta
     3661 acctcttccg ccttactgat cggaattttc ataacgtacg cgaggtcgtt cgtaatatga
     3721 tctccgccta tcgggataat cgacagttta acagggcgtc cgtcggtata gagggcgact
     3781 ccggtagttc ctcctccgat gcacaacgat atcaccccgg cgcgtgtctc ttcgtcggtt
     3841 aaggatccaa gagcactggc aagtgatttt attaccagtc cattgacctc aataccagcg
     3901 ctttcgacgc agttgattat attttggacc acagttgtcg gaacaattac cgattgcaat
     3961 tccatttcga gacgaatgcc cgtcatacct agaggatcgt ctattccgga attaccgtcg
     4021 attgagtatt taacaggaat ggtgtgcaat gtgacacgat tcgggggtat tgagagctcg
     4081 ctctgcgctg cttcaataac ccgttccaca tcggagatag agacctggcg aggcgttctt
     4141 ccaagagaaa tcatccctct tgaagttacc gaagcaactt ccatcccgct gaaagaaacg
     4201 gttgcttcgt ctatttcaaa accaaccatg ttctcagcgt cttttattgc ttgacgtacg
     4261 gatcgcacag cctgttcaag atttatgatc agtccttttc ggatgccttg ggacggcgcg
     4321 taccccacgc caataatttg agcttcctgg aaacgggaat ccctctctgc aataacgacg
     4381 gatattttac tcgtaccaag atccagccca accaaaattt ccggctcttt gtacacttat
     4441 ctcccgctcc cttcctgatc gccgcgcgcc atgccccgga cgataattct gtcggtatat
     4501 gtggtatcta ttataatgtt gttgccgaga aaatgtaact gctccattat ctgcgaaagc
     4561 gcggatgaaa gttctttcca acggggacgg tctccccata aaagaacgtg cgctcctcct
     4621 ttatcgaaaa cgagttttaa taaaaacttt ccttctctcc tcgaaacgct tatttgctca
     4681 actcgagcgc cccacctgtt ttctgccaag ccatccaacc aaccctggag aagctccagc
     4741 gggaaaacta ctctggaaac aatagcagaa gagttttcca aagcaggaga agggaaatcg
     4801 tcagaaaaag ttatcaaagg tgcattggga cgcacaatac ccctgatcat tttgttcagt
     4861 tcatttgcgg tgtcccaagc aaaaccgtct tgagaaacat aatactcgcg tttgttccat
     4921 tgagctacaa tccaaggtcg tagcggctct atctgaactt caactcccgt gattcccctt
     4981 cggcttatcg tacaaaagag aggagcctgt atctcgactg cctttcgcaa atcatctttt
     5041 tccaagaaaa acgcgggcca gaatcgcaac gcgcgcgaac tgatattctt ccagaaaaac
     5101 aactccgcta cggggtcctc agattcaata cgaatattac gtaaagaaaa cggttgaaaa
     5161 aagtcttctc ccccgaagag cattcccata aagaaggcga aaagaaaaaa taaaccgcgc
     5221 catttaccca ttatacatca caaaaatggc tcgagtaaaa agcaggaaag caataagaaa
     5281 agaattcact tttcctcaaa cccctgaacg ctggtttgga aaattacaca cgcactaaat
     5341 gtgtctttgc ctttgcaagc aaattgtctc ctattaacga cacgtctcct gcaccaatgg
     5401 tcaagacgat gtctccttcc tcgcaaaggt cttccaaaag gaaaacagca tcatcaagag
     5461 tttttggcat caggcacgct tgctttttct cggccggtag atggtcggaa attaggagcg
     5521 acgttatccc agagatcggt tcttcgtcag cctggtagat cgggagaaga atcagtgtgt
     5581 ctgcgactgc aagaacagat gcgaagtctt taaacaaagc ctgggtcctc gtgtatctgt
     5641 gcggctggaa gacaacccga agcggccttt cagggaacat atgttccagc gttcccaagg
     5701 tggctgcgat ctctcgcggg tgatggccat agtcgtcata gacgtcgata ccctcaaaac
     5761 ttccgacatg ctggagacgg cgtttcgccc ctttaaagtc ccgcagggtt cttttaatga
     5821 caggaaacgg gattccgaga cgatgtgcaa ccgcacagga agcgagcgtg tttaatatgt
     5881 tatgttctcc agatattgcc agatcaacct cgcctactcg ttgtccctcg tgcagaagcg
     5941 tgaaactgac gccgccgccc ggtttgtgat agacattgct tgccccccaa ttccaagaag
     6001 cgccccagcc gtacgatatg gctccctcag gaatacggcc aagggacagc aagcgcgcaa
     6061 ggcccgcatc ctcaccgcag aaaacaagga gaccgtctga tttgagattg gagaaaaaac
     6121 gtgaaaaagc ttcaagaaca ctttcaaacg tagggtaaaa attgacgtga tcccagtcaa
     6181 cgtttgtaat gacggaaatt tcgggatgaa atttctcaaa agaaccgtcg ctttcatcga
     6241 gttcggcaac cataaagcgt ccttctccaa gttttgcgtt gacgccgata tcacacaact
     6301 ctcccccgat agccagggtg ggatgaaaac cggcctgttc caaaataagc gagatcattg
     6361 aagacgttgt ggttttccca tgagttccgg caacccctat tccgcaacgc gaatcaaaaa
     6421 tccagctcag tacttctccc ctttttgcta cagcgatccc gcgtgctctg gcagcgagga
     6481 tttcttcatt gtcttccggg atggcgctgc tgtatattaa aagatccgga agaaactttt
     6541 ccatatgagc atgtccgtgt cccagaacca ctgccacttc ttccttgagt accttgtcga
     6601 tataagaagt atggatcatg tcgcagccgc tcacttcaaa acccaattca gccagcaaaa
     6661 gggccaatcc gctcatacct gcgccgccga tgcccatgag atgaattctg tgaatgccat
     6721 caaaattcga tatagtcgta tccacttata aaatctccct tccaatactg gacgagatca
     6781 gccgccataa agattcagac tcatctccct tcgaaatact tcgtggtggt ctctgtgtat
     6841 ttcgaagaat tttcagtatg cactcttgaa acgctttcgg ggaatcattt tcatgccaga
     6901 tagcgccgtc attgtgttca ataaaacaga gagcgtttct ctcctggtga ttatcggcag
     6961 cttcttgcca cggaacaaca accgcaggaa taccgagggt tgctaattca gaaagcgtag
     7021 aagcaccccc gcgagtcact acggcgtcga caacagagta gaaattgctc atatcccatt
     7081 gtttaccgat aacagatata ttttccgggc tgccatgaaa ctcccttgct atgtcggcaa
     7141 gctgagaatc gccgaggaac aaaaagaaaa aattcctcat ttcctgcgcc cgaagcatgg
     7201 gacccattaa ttgtatcagt cgttggctca taagtgaacc tccgagaact ccgattattt
     7261 ttttctctgg aagttttttc cctaaagcca ttaattccca agcttctttc ggttctctca
     7321 aagagaaacc gcgaaccgga acgccggtcc agtggaaagc gtccggagaa agaggagtgc
     7381 agacttccca tcctgaagcc acctttttcc caagacgtga agaaagccgt gtcactttgc
     7441 ctgcaacgct gttttgttca tgaacaaaaa ctggaacgcc tgtaagatga caaaccatta
     7501 atggaataaa cgaaacgtac cctccgaaca gaacgcatgc atccacatgg cagttcctta
     7561 ataatttttt aaatgatata aaagacaaac caaattcctt caaccggcgc atcttaccgg
     7621 aaagagagcg tattcccaaa ggagagccgc ttgtcggaag ataatacgga gtaatgtggg
     7681 atg
//
LOCUS       DAOPKU010000073         7625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10272_length_7625_cov_4.390092, whole genome shotgun sequence.
ACCESSION   DAOPKU010000073 DAOPKU010000000
VERSION     DAOPKU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10272_length_7625_cov_4.390092"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK849_03500"
     CDS             complement(<1..205)
                     /locus_tag="PK849_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65222.1"
                     /translation="MTKCSISFSGERAPASETVLSDAREGWSLFSIHDRVFCIDISGS
                     RLWELFPAPRGESLAMLSLFSPSI"
     gene            complement(1266..1484)
                     /locus_tag="PK849_03505"
     CDS             complement(1266..1484)
                     /locus_tag="PK849_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliR"
                     /protein_id="HPE65223.1"
                     /translation="MPIINIQALIALALFLASLFIARIVVRIQNGSLPGGALWVLYLR
                     MLLGFLFAGAIVLAFYSFAGIDIISKHL"
     gene            complement(1683..2660)
                     /locus_tag="PK849_03510"
     CDS             complement(1683..2660)
                     /locus_tag="PK849_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPE65224.1"
                     /translation="MDLKRVGVIGVGHLGQHHARVYTELLGAHLVGVVDQNESRAAAI
                     GENLGVPWFTEIDDFIDRAAPEAVSVVVPTSMHFEIARKALKNGIHVLIEKPVTTTVN
                     EAEQLLRIAAESDLVLQVGHIERFNSAVQYISKIVHEPLFLQSRRLGPYSPRISDVGV
                     VLDLMIHDIDIILSLVRSEISDISATGRCIRSDHEDIASAQISFANGSMAHILVSRVS
                     ERRLRQLEIMEPERYVTIDYETQDVSINRCVRQNNNSLVEVIEHPVFPKSEPLKLELQ
                     HFVTCVREGKQPLVGITDGKRALEVAVSVLKQIHLDDQQFPEGRQAVSC"
     gene            complement(3500..4879)
                     /locus_tag="PK849_03515"
     CDS             complement(3500..4879)
                     /locus_tag="PK849_03515"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPE65225.1"
                     /translation="MKRPFSTFEDLEDFFSRLASPGIRPGLERIARLLKQLGNPEKRF
                     SAIHIVGTNGKGSTAAFTERILRESGYTTALYTSPHLESPAERLLFSGVPLSLEEWSA
                     CAFEVEDALNDDPTLREDPPTFFEIVTATAFLLCEHRGVDIAVVEAGLGGRLDATNLL
                     GNICLTLITSISMDHMDFLGDTLESIAGEKFAVMRTRTPAVFSGTPAQLVPLFRSTAN
                     ELGALPNTIGELCSCEEIRNESGRLSYVYRLLHPALLSSIGGEAKSFDQNAVIPVSTS
                     LRGMYQIENSALAISGILLLVSCFPKITSNTIRRGIAAARWPGRFETIDSTPPLVLDG
                     GHNPGGVEKLTESLGAVYGGKKIGVVYGCMKDKAYPECLELLRKVCSSLYCVSVPENP
                     RAAQPELLAETARSHGWKPEDISVLHDPLEAIRVASLSNEVVVCCGSLYLIGYVKSRL
                     SFLKKERNE"
     gene            complement(4885..7569)
                     /locus_tag="PK849_03520"
     CDS             complement(4885..7569)
                     /locus_tag="PK849_03520"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPE65226.1"
                     /translation="MADIMESMAKSYDPEPIEDKWYARWIDSDLFKARVDKSKEAFSI
                     VIPPPNVTGSLHMGHAFNHTFQDIVCRFKRMRGYNVLWLPGTDHAGIATQNVVERRLA
                     GEGKTRHDLGRDEFVQRVWEWKDEFGSRIINQMKKLGNSCDWDRERFTLDEGLSKAVR
                     SVFVQLYKKGLIYKGKYIINWCSRCHTALSDIEVEHEECKGRLYYVRYPFVDGEGSVV
                     VATTRPETIWGDVAIAVHPRSEKGALVGRKVRVPLGGREIPIIEDIMVDPEFGTGCVK
                     ITPAHDPNDFLVGQRHNLEQVQVIDENGIMNSLAPGYEGLNIEEARKNAVEDLQASGH
                     LGTIEEIVHSVGHCYRCHTTVEPYLSEQWFVKAKPLAEAGVKVVQDRKIRWVPDQWEK
                     VYYQWMENIRDWCISRQLWWGHRIPAWTCGACGHITVSESDPTVCERCGSAAIVQDED
                     VLDTWFSSALWPFSTLGWPEKTEELAYFYPTSLMVTGFDIIFFWVARMIMMGLEFMDE
                     IPFHDVYIHALIRDEKGQKMSKSSGNVIDPLDMIEKYGADALRMTLAALTVQGRDILL
                     STSKIETYRLFMNKIWNASRFALMNLEGLPHEAVLSDDSELALHDRWILTRTSQVAGE
                     TTRLIEAFGIGEAARMLYDFVWGDLCDWYLELSKPALKGAEGPGRKLATQLVIASVFK
                     DVLLLMHPFIPFVTEELWQVFGFGKDAGGNSPFMECHPWPTGDDAFSFPESLEKMAAF
                     QEVVRVLRNLRAEARVTPQTFVNRAVLQCGEESSLSLIIHETAPLMQDLTKIREISLL
                     PLSADKPAGALSSQITGGEVSLIVGDILDIASEIARLSEEIASVTKNMQACSRKLENE
                     DFVRRAPADIVEKERLRLEENEARLRRIHENIESLKRS"
BASE COUNT         1893 a         2121 c         1904 g         1707 t
ORIGIN      
        1 agattgaagg ggagaaaaga gagagcatcg cgaggctctc tccgcgaggc gccggaaaca
       61 gctcccaaag tcttgagccg gaaatatcga tacaaaaaac ccggtcatgg atcgaaaaga
      121 gactccagcc ttcgcgcgca tcggaaagta ccgtctccga cgccggcgcg cgctctcctg
      181 aaaaagagat gctgcatttc gtcacatttc ccgactcatg gagaataaaa agatctccca
      241 atgaagacca ttcaaggtcc cgcggggtat ccgcctctat cacaaagcgg cgttgcgcat
      301 caagaaagga ctgaacatac accttggaat tattcctgtc tgcgactgca atccaccctc
      361 catatacggc aatggaaacg ggttcgaaaa gccgatcccc ttccggaaac tcgagccgtc
      421 cggaaggtct gagttccgga agcgagtaca gggtgatttc tctggacgtc gtgctggaaa
      481 ccgcaagcag gctgtttgtc actaacgctg cgtctgagag gcccgttgaa attttcccta
      541 tccgtaccgg actacgttcc ccttccggaa gagaaaagac ctccccgttc tctgatacta
      601 cataaaagct tttacctcct gttggaagcg caaccaccgg ggagggtatc tcgacaaact
      661 ggaaaggctt ttcgccaggg agacgaaaac ccactctgtt cccaaaggta tccgaaagga
      721 aaacgccgtc tgaatacgac gttgctttcc ccagaccgtt tacgcctgga atttgcagag
      781 ttcccagcga aaaagccgtt ttgacatctt tcctggagga gacaactgca gattgtaaag
      841 gttcccctga aaggaactgt tctatgaaaa attgctcatc ccgatagcgt tccaaaattc
      901 tgggggcagc gctgtccctt gggcgaactt ccaggtagta acgtatcgac ttcacagcct
      961 cgtcggttcg tccgatacga tgaaggacga gaccgcgcag aagatagtag tcaacaagat
     1021 acgtattcag cttcaatgct ctgtctagat tttcaagcgc agaagcatac tcccttttaa
     1081 taaagtcttc gtaccccagc gcaaaaaaac gctccgattc ctctctgtcg gctgggggaa
     1141 gagtggcgcc gtccgtcggc gctccagaga aaaggacgga aaatagaaga agtacaatga
     1201 tatgcctcct caggcgtttg aaaaacaaca tcgtctcttt cgtccccttc ccaagccact
     1261 gcccatcaaa gatgttttga aattatatct atcccggcaa aagaataaaa agcaagtaca
     1321 atcgccccgg caaaaagaaa accaagaagc attctcaggt agagaaccca cagagccccg
     1381 ccaggcaacg aaccattttg aatacgcaca acgattctcg ctataaacag cgaagcaaga
     1441 aaaagagcaa gggcgatcag cgcttggata ttgataatag gcatggcatt ctccgcttcg
     1501 tgtgtgaatt agggtaatca tgattaggat accctataaa aaaccattac gccacttcaa
     1561 ataaagattt tacctctcgt gcgaggagaa aaaaaagaaa aaaacacatg ctctgtcgaa
     1621 aaaaagtacc ccggatcgat tacctccggg gtaccactat aacacgattt cagagaggat
     1681 actcaacatg aaacagcttg gcggccttcg gggaattgct gatcatccaa gtgaatctgt
     1741 ttcaggacgg aaaccgcgac ttccaaagcc cgtttcccgt cagtgatgcc tacaagaggt
     1801 tgtttccctt cgcgaacaca cgtcacaaag tgctgcaact caagcttcaa gggttcgctc
     1861 ttcggaaaca ccgggtgctc gatcacctct accaggctgt tgttattctg acgaacgcac
     1921 cggttgatcg aaacatcctg cgtttcgtag tcgatcgtga cataccgttc cggctccatg
     1981 atttcaagct gacgcagcct tctttcggag acgcggctta ccaggatatg cgccattgaa
     2041 ccgtttgcaa aggaaatctg cgcggaggcg atatcctcat gatcggatcg tatgcatctg
     2101 ccagtcgccg aaatatcgga gatttcggac cgaacgagcg aaaggataat atctatatca
     2161 tggatcatca gatcaagaac aaccccgacg tcgctgattc tgggggaata gggaccaagc
     2221 cggcgggatt gaaggaagag cggttcgtga actatcttcg agatatactg aacagcgctg
     2281 ttgaaccgtt caatatgccc tacctgaagc acaagatccg actcggcggc gattctgagc
     2341 agctgctccg cctcattgac cgtcgtggtc accggttttt cgataagcac gtggattccg
     2401 tttttcaagg cttttcgtgc aatctcaaag tgcatgctgg ttgggacgac aacgcttacg
     2461 gcctcgggcg ccgcgcggtc gatgaagtcg tctatttcag taaaccatgg aacgccgaga
     2521 ttttctccga tcgccgctgc ccggctttca ttctggtcca cgacccccac aagatgggcg
     2581 ccgagcagtt ccgtgtagac tcttgcgtga tgctggccaa gatggcctac tccgatcacg
     2641 cctactcgtt tcaagtccat ggactcttca actcctttga ttacgtacgg ccttttcgtg
     2701 ctcttacata attctcccgg taaacggagt aaaaatggta ttttttactc cgtcaaaaaa
     2761 gcgaggagaa aaagtctgtt ttccatatcc ccgttcctgt aggagtagaa tatatcgctc
     2821 ctctcaaagg tacacagcgg aagggaacag atattctcag gtaagagccc cgcgtcggtc
     2881 agttggcgga aaatttgccc tccgatatca aaagacacat cagcagaacg tacatctctg
     2941 ttttcaggag agaaaacaga aagccctttt ttcgtccagg ggtcgttctg ctttctatta
     3001 tagctcctcc gtccgattcc tggccccacc caggcatacg ttgcggcggg ccgacgtccg
     3061 ttcgcagaga agatgttttc acagacagta ccgctgatgt tggaaaccgc ccctttgaat
     3121 ccggagtgca ctatggcgat ccagggcgat ggcgccaagg aagcaagaac aacggggaaa
     3181 cagtcggcaa aacgaagaga tccctcgaca ccgggagtgt caagaaaaac gccatcccct
     3241 tccggtcgtt caggtaaagc atacgaaacc tccccgggaa ccatgaaagt gccgtgcacc
     3301 tgatggggag caatcaatgc cccttggcgc ggtattaaca gttcggtaag ctccctggcc
     3361 gtcgcgggat tcccgagagc acgatccatg gccgcccccc gtagaaaaag gcggataaaa
     3421 aaaggcgtat gcggctccgg aaagagatat tcgagaaacg gaaagtctcc attgggctcg
     3481 attctgaaag aaggcgcgat cattcgtttc tctccttttt gagaaaagac aatctgctct
     3541 ttacgtatcc aataagatac agactgccgc aacagacgac aacttcgttc gaaagggagg
     3601 cgacacgaat ggcttccagg gggtcgtgca aaactgaaat atcttcaggc ttccacccgt
     3661 gtgaacgcgc agtctcggcg agaagctcag gctgagcagc gcgaggattc tccggaactg
     3721 aaacgcaata gaggctggag cagacttttc gtagcagttc cagacactcc ggatacgctt
     3781 tatccttcat acaaccgtac accacgccga tcttctttcc gccgtagacc gcaccgagac
     3841 tctccgtcag cttctctacg ccgccgggat tgtgcccgcc gtcaagaacc agcggagggg
     3901 ttgagtctat cgtttcgaat ctgcccggcc aacgcgcagc ggcgattccc cggcggatag
     3961 tattggatgt aattttcgga aaacacgata cgaggaggag gatccctgaa atggcaagcg
     4021 cgctgttttc gatctgatac ataccgcgca aggacgttga aaccgggatg acggcattct
     4081 gatcgaagga tttagcttcc ccacctattg aagacagcaa cgcaggatga agcagacggt
     4141 agacatacga caatcttcca ctttcattgc gaatttcttc gcacgagcag agttcgccaa
     4201 tggtgttcgg gagcgcgcca agttcattcg cggtcgatcg aaagagagga acaagctgcg
     4261 caggagttcc cgaaaagaca gcgggcgttc tcgttcgcat taccgcaaac ttttccccgg
     4321 caatactttc gagagtatcc cccagaaagt ccatatgatc cattgaaata gacgtgatca
     4381 acgtcaggca aatattgccg aggagattcg tcgcatccag tcttcccccc agaccggctt
     4441 cgaccacggc tatgtcgacg cccctgtgtt cacagagcag aaacgcagtc gccgttacga
     4501 tttcaaagaa cgtcggcggg tcttcgcgga gcgttggatc atcatttaag gcatcttcga
     4561 cttcaaaagc gcaggcagac cactcttcaa gcgagagagg aaccccgctg aagagaagac
     4621 gttccgccgg agactcaaga tgcggactcg tatacaaagc cgtagtatat cctgattccc
     4681 ggaggatgcg ctctgtgaaa gcagcagttg atcctttgcc gttcgttcct acgatatgga
     4741 tggcggaaaa ccttttttcc ggattgccca gctgcttaag caaacgggca atccgttcga
     4801 gccccggccg tattccgggg ctcgccaggc gggaaaagaa atcctccaaa tcctcaaagg
     4861 tcgagaacgg gcgtttcatc atctttagga gcgcttgagg ctttcgatat tctcatgaat
     4921 acgacgaagc cgagcttcgt tctcttcgag acgaagacgt tccttctcga cgatatccgc
     4981 cggagcgcga cggacgaaat cttcgttctc gagctttcgg ctgcaagcct gcatgttctt
     5041 tgtcacagac gctatctctt ccgaaagacg ggctatttct gaagcgatgt cgagaatatc
     5101 ccctacaata agactgacct ctcctccggt tatctgggaa gagagcgctc ctgcgggttt
     5161 gtccgcggag agcggcagga gggatatctc acggattttt gtcagatcct gcataagcgg
     5221 cgctgtttca tggataatga gggacagcga actctcctcg ccgcactgaa gaacagcgcg
     5281 gttcacaaag gtctgcggcg tcaccctcgc ctctgcgcga aggttgcgga gcaccctgac
     5341 gacttcctgg aacgccgcca tcttctccag cgactccggg aaagagaatg catcgtcgcc
     5401 tgtcggccac ggatgacatt ccataaacgg agagtttccg ccggcgtctt ttccaaaccc
     5461 gaaaacctgc cacaactcct ccgtaacgaa cggaatgaag gggtgcatga gaaggagtac
     5521 atctttaaag acggatgcga tgacaagctg cgtcgcaagt ttccggccag gaccttccgc
     5581 gcctttgagc gccggttttg aaagctccag ataccagtcg caaaggtctc cccagacgaa
     5641 gtcgtacagc atccttgccg cctcgccgat accgaaggct tcaataagcc gcgtcgtctc
     5701 tccggcgacc tggctggtcc gggtaagaat ccatctgtcg tggagcgcga gttccgaatc
     5761 atctgaaagg actgcttcgt gcggaaggcc ctccagattc ataagggcga aacgcgacgc
     5821 attccaaatt ttattcatga agagcctgta tgtctctatc ttgctggtgc tcagcagaat
     5881 atcccttccc tgaacggtga gcgcggcaag cgtcatccgg agagcatccg caccgtattt
     5941 ttcaatcatg tccagcggat ctatgacgtt cccgctcgac ttgctcatct tctgcccttt
     6001 ttcatcgcgg ataagcgcgt ggatatagac gtcatggaac ggtatctcgt ccatgaactc
     6061 caaccccatc atgatcatcc gcgccaccca gaagaagata atatcgaacc cggtcaccat
     6121 aagagaggtc ggatagaagt acgcaagctc ctctgtcttc tcgggccatc cgagcgtaga
     6181 aaatggccac agcgcgctgc tgaaccacgt gtcgaggaca tcttcatcct ggactatagc
     6241 ggcgcttccg cagcgctcgc acaccgtcgg atccgactcg gaaaccgtga tgtgaccgca
     6301 cgctccacag gtccacgccg ggattctgtg tccccaccag agctggcgtg agatgcacca
     6361 gtctctaata ttctccatcc actggtagta gaccttctcc cactggtccg gcacccatcg
     6421 tatcttcctg tcctgtacga ctttgactcc agcctcggca agcggcttcg ctttgacgaa
     6481 ccactgctcg gaaagatagg gctcgaccgt ggtgtggcac cgatagcaat gccccaccga
     6541 gtgcacgatc tcctctatcg taccaaggtg tcccgaagcc tgaagatctt cgactgcatt
     6601 tttccgcgcc tcttcaatgt tcagcccctc gtatcccgga gcaagagagt tcatgattcc
     6661 gttttcgtcg ataacctgca cctgttcaag gttatgccgc tgtcccacca ggaagtcgtt
     6721 gggatcgtgc gcaggagtta tcttgacgca ccccgtgccg aactccgggt ccaccatgat
     6781 gtcctcgata atcgggattt cccttccacc gagaggaacg cggacctttc ttccaacgag
     6841 ggcgcctttt tccgagcggg gatggactgc gatcgccacg tcgccccata tcgtctcagg
     6901 acgtgtcgtt gcgacgacaa cgctcccctc gccgtcgacg aaaggatagc gtacatagta
     6961 aaggcgccct ttacactctt cgtgctcaac ctcgatatcg gagagcgccg tatggcaacg
     7021 tgagcaccag ttgatgatgt actttccctt gtaaatgagt ccttttttgt agagctgaac
     7081 gaagacggaa cggacggcct tcgagagccc ctcgtcgagc gtaaaacgct ctctgtccca
     7141 gtcgcaggag tttcccagct tcttcatctg gttgatgatc cgactaccga actcgtcctt
     7201 ccactcccag accctctgta cgaattcatc cctgccgaga tcgtggcggg tcttcccttc
     7261 acccgccaga cggcgttcca cgacgttctg cgtggcaatc cctgcatggt cggtgccggg
     7321 cagccagagg acgttatacc cgcgcatacg tttgaagcgg cagacaatat cctggaacgt
     7381 atgattgaag gcatgcccca tatgaagtga tccggtgaca ttcggcggag ggattacgat
     7441 agaaaacgcc tctttgctct tgtcgacacg cgccttgaaa agatccgaat caatccagcg
     7501 cgcataccat ttatcttcga tcggttcggg gtcgtacgat ttcgccatag attccataat
     7561 gtctgccatg ctgcaattcc cctccgttgc ccaaaaattt gcttcctcgg cagtatttca
     7621 ataga
//
LOCUS       DAOPKU010000074         7573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10368_length_7573_cov_5.140862, whole genome shotgun sequence.
ACCESSION   DAOPKU010000074 DAOPKU010000000
VERSION     DAOPKU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10368_length_7573_cov_5.140862"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PK849_03525"
     CDS             complement(<1..442)
                     /locus_tag="PK849_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65227.1"
                     /translation="MEIENVLFAFGLTLFAGLSTGIGSALAFFTKRTNTRFLAFSLGL
                     SAGVMIYVSMVEIFFKAQEALQADLGERMGAWVTVVSFFLGILGVMLIDKFVPSHENP
                     HEMHRIEEIRGRAGELGQEHALMRMGILTALAIAIHNFPEGIATF"
     gene            complement(530..2623)
                     /locus_tag="PK849_03530"
     CDS             complement(530..2623)
                     /locus_tag="PK849_03530"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HPE65228.1"
                     /translation="MDIDFVTDSSYEVRFEDMFDLADIQRLQDEFSDATGVASVVTRP
                     DGTPLTRPANFTRFCSKVVRGAEKGCANCFRSDALIGRYNPKGPTIQRCLSGGLWDAG
                     AGISVGGKHVANWLIGQVRDESQNEEAIRAYAREIGADEEEAVAAFREVPAMSRDRFQ
                     KIARMLFTLVQQLSELAYQNVRQTLFIAEKNRAETIRKVQHELAEVMATAGSEIEVFD
                     MARRELAGLMETKNFLIVDYDETTRMLSTRMYLGVDEKKLPVRWSADKALTGIMMAAR
                     KAMHFSRTEIIRLVDEGLIEFVGDRAETWVGVPLQNEKKVFGAMILQSYTDPYAYDDW
                     CVEILQSVGNQLSVYLEKKRAEGALRESEEMFRAFFEQNSVGFAMINTDGSWLRFNDK
                     LCEMTGYSREEMQEMNWRDFTPPEEQQRERPLLNDLISGKIDSYTIEKRCIRRDGSTI
                     DIFVLTKPLRRPDGEFRYFASIIQDISERKKSERELARHQEAIIGSMAILAERRDHGT
                     GEHIQRTKFYVQRLLELSGGERFYPLEHLPLLAQAAILHDIGKVGVPDSLLLKPGKLT
                     PEEFEVVKQHTVIGGDALAAAARILGEAPFISYARQIIEYHHEKWDGTGYPHGLKGEE
                     IPFIARIMAIADVYDALVSERPYKGPIDHEKAVAIIVEGAGTHFDPVLVEAFLACADE
                     FKSISQSGDTPVPGR"
     gene            complement(2889..3626)
                     /locus_tag="PK849_03535"
     CDS             complement(2889..3626)
                     /locus_tag="PK849_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE65229.1"
                     /translation="MNQWIAFWNILLKDMRTYYMKPPNISWGLLFPLAWTGMFFIRSG
                     SGLESIPALLPGVTALSILFGSTSVLAVTVTFEKKNRSFERLLLAPISFELLMLAKTS
                     GAILFGVLNAFVPVLMASFVADLSPIDWRAFVPAVVLLSTASTFLGLFIAVAVSEVFE
                     AQTFSNFFRFPMIFLCGLFFPIERLPVFLRPLSYALPPTYGVDMLRGAVGGVHTMPYL
                     LNLAALCVFCAGLFALSLRTIRRSWIA"
     gene            complement(3685..4047)
                     /locus_tag="PK849_03540"
                     /pseudo
     CDS             complement(3685..4047)
                     /locus_tag="PK849_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
     gene            complement(4270..>7573)
                     /locus_tag="PK849_03545"
     CDS             complement(4270..>7573)
                     /locus_tag="PK849_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65230.1"
                     /translation="RPQRVPIRGSTRKRILPAGWRRRSFFRTIGFCVLLFWLPLLAPG
                     IAECADRNAKAHILILHSYHDALPWSKGLNRGIENAFAHAPFPIELYVEYLDAIHHPG
                     EDILDETTALFKKRYEDIPLDAVLLTDDPAWTFLVERRDLLFPGVPLFFCALNDLHPE
                     RLEGISAVTGIAENPDMSGTVELVRKLHPGIREIPVVADRNPGSLLVLKNLERISREL
                     DGEVTFRALTDAGFPELLEELKRLPEGTPVIFHAFLRDASGATFPSNLTVLGELSETI
                     SSPFYTFKRIDIGHGAVGGAVVSEELLGSLTARMLVDHLNGVPLEELPPVFDTPHAYI
                     FDEAQLERFGIDEQLLPQGSVVVNRHLSLYETHKKAIWGVAAVVGILLGLVVLLALNV
                     LRRLRVEKMIVSINRNLEERVRDRTQELDRALLRAESANIAKSEFLANMSHEIRTPMN
                     GVIGMTDLLLETPLTLEQRRYALMLQQSAESLLELLNDILDFSKIEAGKLDLELIDFD
                     LHDLLGDIASAQAVRASEKGVEVLCAIDPDVPSSLFGDPGRLRQIVNNLAGNAVKFTA
                     EGEIEIGVSLTSEDEESATLAFRVRDTGIGIPEEKRDMLFKKFSQLDSSTTREYGGSG
                     LGLAISKQLVGMMGGEIGVESPAPSAGEASGGPGSLFHFTVRLKKSTATRTTARPPAE
                     LQDIRILVVDDNATNREILAAYIAAQGIRSAEAANGADALRLLRRGAEEGDPFHVAVI
                     DMHMPLMDGETLGRTIKADPQLAKTCMIMLTSLGMRGDARRFADAGYSAYLTKPVRCR
                     ELMGVVVPALRERYWSETESAPLLTRHTVRKQLEPCDTKVRVLLAEDNVTNQQVAVGI
                     LEKFGIEVEIARNGEEAVEAVRRRSFDLIFMDIQMPLMDGLEATKRIRGMHAASGEPA
                     VPIVAMTAHAMEGDRERCIEAGMNDYLSKPVKPAAMFAMLRKQLPAKDLLPCTYTASS
                     AVDDKKVDADVSVWNRTPFYDRLLEDRELAHRILEDFLIDIPSQIRQLRATLKKGDSE
                     GARRRAHAIKGAAASVNGESLRLAALELERRALAGEQQELAFLADRLEASFEDLRAAM
                     LKELRNGTKSHP"
BASE COUNT         1457 a         2264 c         2271 g         1581 t
ORIGIN      
        1 tgaaggtggc gattccttcg gggaagttgt ggatggcgat ggcgagcgcc gtgaggatgc
       61 ccatgcgcat cagcgcgtgc tcctgcccaa gctcgcctgc gcgcccgcgg atctcctcta
      121 tccggtgcat ctcgtgcggg ttctcgtgag aggggacgaa cttgtcgatc agcatcactc
      181 ccaaaatgcc gaggaagaag gagacgacgg tgacccaggc gcccatcctc tctcccagat
      241 cggcctggag cgcctcctgc gccttgaaga agatctccac catcgagacg tagatcatca
      301 ccccggcgga gaggccgagc gaaaaggcca gaaagcgggt gttggtccgc ttcgtgaaga
      361 aggcgagcgc gctgccgatt ccggtcgaga ggccggcgaa cagcgtgaga ccgaatgcga
      421 agagtacatt ctcaatttcc atgtgacgac gacctccttg atatgatgtc acgtgtacct
      481 gaacacacaa taccgaaacg aagcgtcggc gtcaagcgag ggcatttctt tagcggccgg
      541 gtacgggggt atctccggat tgggagatac ttttaaattc gtcggcgcag gccaaaaagg
      601 cctcgacaag gacggggtcg aaatgggttc ccgccccctc gacgatgatc gccacagcct
      661 tctcgtggtc aatggggccc ttgtaggggc gttcgctcac gagcgcgtcg tagacgtcgg
      721 cgatcgccat gatcctcgcg ataaagggga tctcctctcc cttcagtccg tgcggatacc
      781 ccgtgccgtc ccacttctca tgatgatact cgatgatctg gcgcgcatag ctgataaacg
      841 gcgcctcgcc gagtatcctt gccgcggcgg caagcgcgtc gccgccgatc acggtatgct
      901 gtttgacgac ctcgaactcc tcgggcgtca gcttgccggg cttcagcagc aggctgtcgg
      961 ggacgccgac cttgccgatg tcgtgcagaa tggccgcctg cgccagaagc ggaaggtgct
     1021 caagggggta gaagcgctcg ccccctgaaa gttcgagcag ccgctgaacg taaaatttcg
     1081 tgcgctgaat gtgctcccct gtgccgtggt cgcggcgctc cgccaggatg gccatgctgc
     1141 cgatgatcgc ctcctggtgg cgtgccagct cccgttcgct ttttttgcgc tccgagatgt
     1201 cctgaatgat ggaggcgaag taccggaatt ccccgtcggg gcgccgcaga ggttttgtaa
     1261 gcacgaagat gtcgatcgtc gaaccgtccc gacggataca gcgtttctcg atcgtgtacg
     1321 aatcgatctt tccggagatc agatcgttca gcaaagggcg ttcgcgctgc tgttcctcgg
     1381 gcggagtgaa atcgcgccaa ttcatctctt gcatttcctc ccgggagtag cccgtcatct
     1441 cgcacagctt gtcgttgaag cgcagccacg aaccgtccgt gttgatcatg gcaaagccga
     1501 cgctgttctg ctcgaagaat gccctgaaca tctcctcgct ctcgcggagc gctccctcgg
     1561 cgcgtttctt ctccaggtag acgctgagct ggttccctac cgattgcaga atctcgacgc
     1621 accagtcgtc gtacgcatac gggtctgtgt aactctgaag gatcatggcg ccgaagactt
     1681 ttttctcgtt ctgcagcggc acgccgaccc atgtttcggc ccgatcaccg acgaattcaa
     1741 tgagcccctc gtcgacgagc cgtataatct cagttcggga aaagtgcatc gcctttctgg
     1801 cggccatcat aatgcccgtc agcgctttgt ccgcggacca tcgtaccggc agcttttttt
     1861 catccacgcc cagatacata cgtgtcgaga gcattcttgt cgtttcgtcg taatcgacga
     1921 tcaaaaaatt cttcgtctcc atcagcccgg cgagctctct tcgagccatg tcgaacacct
     1981 cgatctccga gccggccgtc gccatgactt cggcaagttc gtgctgtacc ttcctgatgg
     2041 tttccgcgcg gtttttctcc gcgatgaaaa gcgtctgccg gacgttttgg taggcgagtt
     2101 cggagagctg ttgtacaagc gtgaagagca ttcgtgcaat tttctggaat cggtccctgg
     2161 acatggcggg aacctcgcgg aaggcggcga cggcttcttc ctcgtccgcg ccgatctcgc
     2221 gggcgtaggc gcgtatcgcc tcttcgttct ggctctcgtc gcggacctgc cctatcagcc
     2281 agttggcgac gtgctttccc ccgacggaga ttcctgcgcc cgcgtcccag aggcctccgc
     2341 tgaggcagcg ctgaatcgtc ggtcccttgg ggttgtaccg cccgatgagc gcgtccgaac
     2401 ggaagcagtt ggcgcacccc ttttcggcgc cgcgcacaac cttgctgcag aagcgggtga
     2461 agttggcagg gcgcgtcagc ggcgtcccgt ccggccgcgt gacgaccgag gcgacgcccg
     2521 tcgcgtccga gaactcgtcc tgcagacgct ggatgtcggc gaggtcgaac atgtcctcga
     2581 agcggacttc gtaagagctg tccgttacaa aatcgatgtc catgacgaaa acctccggag
     2641 atagtcgttt attcacgcta cttccttccg ggagagatac ggcaattccc caaagagcgc
     2701 gcagagagag ttttctgcgg cgccccgtat gcgcatacac aatcttccga tggtttgtat
     2761 tatagactgt caaggggtat ttcgcacctt ttgccttcca aggcgagcgc aagaaagagt
     2821 gtgcgcttcc aaaaaagagg aggtgcaaga gaatcacgca gttattcgag agaaaaggag
     2881 gcggcgcgct acgctatcca gctcctccgg atggtccgca ggctgagggc gaagagcccc
     2941 gcgcagaaga cgcagagcgc ggcgaggttc aggaggtacg gcatggtatg gacgccgccg
     3001 acggcccctc tgagcatgtc caccccgtac gtaggtggga gcgcgtagga gagcgggcgc
     3061 aagaagacgg gcagcctctc tatggggaag aagagcccgc agaggaagat catcgggaag
     3121 cggaagaagt tggaaaacgt ctgcgcctcg aagacctcgc tgaccgccac cgcgatgaag
     3181 agcccgagaa acgtggacgc ggtcgacagc agtacgaccg ccggaacgaa cgctctccag
     3241 tcgatcggcg ataaatccgc gacgaagctc gccatgagca ccgggacgaa ggcgttcagc
     3301 acgccgaaga gaatggcccc ggacgtcttc gccagcatca gcagttcgaa ggatatgggc
     3361 gcaagcagca gccgttcgaa cgagcggttc ttcttctcga acgtcaccgt caccgcgagc
     3421 accgaggtgg agccgaagag gatggagagt gccgtcaccc ccggaagcag cgcgggaata
     3481 ctctccaggc cgctccccga cctgatgaag aacatgccgg tccaggcgag tggaaagagc
     3541 agcccccagc tgatgttggg cggcttcatg tagtacgtgc gcatatcctt gagcaggatg
     3601 ttccagaacg cgatccactg gttcacggct attcctccct tatgcgggtc tctcgttagt
     3661 gcaggagcgc gggtgcggcg ccgtcgcttc caccgtgtcg gccacggcga cgctcctcgc
     3721 ctcaagggga atttcctcgc tcttcagtcc ttgcggatac ccggagccgt ccagccgttc
     3781 atggcgccgc tgcaagatat cggcgtgtcc gtcggcggag agggagccca ctttttcacg
     3841 ggcgggctgc acttcctccg gagggatcaa ctccgaaaag tgacggccaa gcagttcgct
     3901 ccggtcgatt tcaagcgccc ggcagagcgc ctcgttggcg aaggtgacgg ttgtatcggg
     3961 cagaaaacgg cagataaggt ccggactcga ctcgacgaga tctttataga tgagagcatc
     4021 ggaagcaaga agagtgtttt tttgcatagt gaaacctccg cgtcgagttc ggtttgagct
     4081 ccggatactg ttctcccttt ttcaaaaaca aaaacgaggc gttccagaac gcctccggtc
     4141 gtcggctcat accttgtatg acatttctat cacatttaat gtattttcac aatatgtctt
     4201 tatgttcttt cgttcaactt ccccgaaccg ggaagtaaaa agagcgagtc ctcccatatt
     4261 cggagagtgc tatggatgcg atttggttcc gttccgcaac tccttcagca tcgctgcccg
     4321 caaatcttca aaactggctt cgagacggtc cgccagaaat gcgagttcct gctgttctcc
     4381 ggcgagcgcg cggcgttcca gttccagagc ggccagccgt agggactctc cgttgacgct
     4441 tgcggcggcg cccttgattg cgtgcgctct tctgcgcgcc ccttcggaat cgcctttttt
     4501 cagcgttgcg cgcagctggc ggatctggct cggaatatcg attaaaaaat cctccagaat
     4561 tctgtgcgcg agctctctgt cctccagcag acggtcgtaa aacggtgtcc ggttccagac
     4621 ggatacatcg gcgtcgactt tcttatcgtc aacagcggaa gaggcggtgt acgtgcaggg
     4681 gaggagatcc ttcgcaggga gctgttttcg cagcatagcg aacatcgccg ccggttttac
     4741 aggtttggaa agatagtcgt tcatccccgc ttcgatgcag cgctcccggt ctccttccat
     4801 cgcgtgcgct gtcatcgcga cgatcgggac ggcgggctct cccgaggcgg catgcatacc
     4861 gcggattctt tttgtggctt cgagcccgtc catgagcggc atctgaatgt ccatgaagat
     4921 caggtcgaag ctgcggcgcc ggacagcctc gacagcctcc tcgccgttcc gggctatctc
     4981 gacctctatg ccgaacttct ccagaatacc caccgccacc tgctggtttg tcacattatc
     5041 ttcggcgagc aaaacacgta ctttcgtatc gcagggctcc aactgctttc ggacggtatg
     5101 gcgcgtcaga agaggcgcgc tctccgtctc gctccagtat ctttcccgga gcgccggaac
     5161 aacgaccccc ataagctctc tgcaacgcac cggcttcgtc aggtacgcgg agtaccctgc
     5221 gtccgcgaag cggcgtgcgt cgcctctcat tcccagcgag gtgagcatga tcatgcacgt
     5281 tttggcaagc tgcgggtccg ctttgatcgt ccggccgagc gtctctccgt ccatcagggg
     5341 catgtgcata tcgatgacgg cgacgtggaa cggatcccct tcctccgctc cccggcgaag
     5401 cagacgaagc gcgtcggcgc cgttcgccgc ctcggcggag cgtatccctt gggcggcgat
     5461 gtatgcggcc aggatctcgc ggttcgtcgc attgtcgtcg acaaccagga tcctgatatc
     5521 ctgcagctcc gccggaggac gtgccgttgt gcgcgtcgcg gttgattttt tgaggcgtac
     5581 ggtgaaatga aagagagaac cggggcctcc ggaggcttct cctgccgagg gggccggact
     5641 ttcgacgccg atctcgccgc ccatcatccc gaccagttgc ttcgatatgg caagtccgag
     5701 tccgctgcct ccgtactccc gggtggtcga cgagtcgagc tggctgaact tcttgaagag
     5761 catgtcccgc ttctcctccg ggatgccgat gcccgtatcc cggacgcgga acgcgagcgt
     5821 cgcggactct tcgtcctcgg acgtcagcga cacgccgatt tcgatctcgc cttcggcggt
     5881 gaacttcacc gcattccccg cgaggttgtt cactatctgc cggagacgtc ccgggtcgcc
     5941 gaagagcgag gatggaacgt cggggtcgat cgcgcagagc acttccaccc ctttttccga
     6001 tgcgcgcaca gcctgggcgg aggcgatatc gcccagaaga tcgtgcagat cgaagtcgat
     6061 cagctccaga tcgagttttc ccgcttcgat cttcgagaaa tccagaatat cgttcagcag
     6121 ctccagaagc gactccgcac tctgttgcag catgagggcg tagcggcgct gttccagcgt
     6181 cagcggagtc tccagaagca gatcggtcat gccgatgacg ccgttcatcg gcgttcgtat
     6241 ctcgtggctc atgttggcaa gaaattcgct cttcgcgatg ttggcgctct ccgctctcag
     6301 aagggcgcgg tcgagttcct gcgtccggtc gcggactctc tcttccagat tcctgttgat
     6361 ggagacgatc atcttctcga cgcgcagacg ccgcaggacg ttgagcgcca gcagcacgac
     6421 gagccccagc aggattccca ccactgcggc cacgccccat atcgctttct tatgcgtctc
     6481 gtagagggag agatgacgat tgacgaccac gctcccttgc ggcagaagct gttcgtcgat
     6541 gccgaagcgt tccagctgcg cctcgtcgaa aatgtaggcg tggggggtgt cgaatacggg
     6601 aggcagctcc tccaagggga cgccgttcag gtggtcgacc agcatccggg ctgtcaggga
     6661 gcccagaagt tcctcgctca cgacggctcc tcctactgca ccgtggccga tgtcgatccg
     6721 cttgaacgta tagaacggag aggagatggt ttcggagagt tcccccagta ctgtcagatt
     6781 gctgggaaac gtggcgcccg aagcgtcccg caggaatgcg tggaatatca cgggcgttcc
     6841 ctccggaagg cgtttcagct cctccagaag ctcgggaaaa ccggcgtcgg tcagcgcgcg
     6901 gaacgtcact tcgccgtcca gctcgcgaga gatacgctcc agattcttca ggacgagaag
     6961 acttccgggg ttgcggtcgg cgacgacggg aatctccctg attccggggt gcagcttccg
     7021 gaccagctcc acagtcccgc tcatgtccgg gttttcggcg atgcccgtca cggcgcttat
     7081 tccttcgagg cgttccgggt gcaagtcgtt gagggcgcag aaaaagagag gtactccggg
     7141 aaagagaaga tcgcggcgct ccaccaggaa ggtccacgcg ggatcgtcgg tgagcaggac
     7201 ggcgtcaaga ggaatatcct cgtacctctt tttgaacagc gccgtcgttt cgtcgaggat
     7261 atcctctccc gggtggtgaa tggcgtccag atattccacg tataattcta tcggaaacgg
     7321 cgcgtgcgcg aaggcgtttt caatgccgcg gttcagtcct ttgctccagg gcagcgcatc
     7381 atggtacgaa tggaggatca ggatgtgcgc tttggcgttc cggtccgcgc actcggcgat
     7441 tcccggagca agaagcggca gccagaaaag gagaacgcag aaacctatcg tccggaaaaa
     7501 agaacggcgc ctccagcccg ccggaagtat tcttttacgg gtgctcccgc ggatcgggac
     7561 tctttgcggc ctg
//
LOCUS       DAOPKU010000075         7570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10373_length_7570_cov_4.199734, whole genome shotgun sequence.
ACCESSION   DAOPKU010000075 DAOPKU010000000
VERSION     DAOPKU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10373_length_7570_cov_4.199734"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PK849_03550"
     CDS             complement(<1..674)
                     /locus_tag="PK849_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPE65231.1"
                     /translation="MVVDLLELLLSLGTSFGFILIVLFILASAVKIVPEYQRVVVFRV
                     GRLVGAKGPGIVLVIPIIDKVVRVDLRVVTLDVPVQEVITRDNVPIKVNAVVYFRVMD
                     PAFSVVEVENYILATSLLSQTTLRSVVGGADLDEVLSSREKINSELQKIIDERTDSWG
                     IKVSAVELKELELPESMKRAMAKQAEAERERRAKIINAEGEFQAAQKLSEAAKQMELS
                     PITLQLR"
     gene            complement(678..2039)
                     /locus_tag="PK849_03555"
     CDS             complement(678..2039)
                     /locus_tag="PK849_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPE65232.1"
                     /translation="MKNCLKKSAMFLVLLFALCACPAFSAQAGTVPSVEGKVFSAALD
                     GTVGVSMENFVAAVLTRAEEEKGGLVIFRLDTPGGLVSSMRAITAAILESSVPVVVWV
                     SPQGARAASAGAFILQAAHVAAMAPGTNVGAAHPVMASGQDAPDKEMKRKITNDLAAQ
                     IRSLAQLRKRNADLAERMVTHSISLTAEEALAEGAADLSAPDLESLLLGVRGRRVLVK
                     GEEKELAPGSGTPVEIIMTSREKALQFISSPDIAYLLLTAGILMIVLEVMSPGGFVLG
                     TAGAVMVLLGSFGLRMLPFNWAGIVLLVAGIGVLVLDLMVGGIGILSLFGLASLVTGG
                     LILFRAPGGELLNVSMSVIGGMSLALGLFFLGALFLVVRSMNKKAVSGREGMKGKKAV
                     VVEDLLPEGLVRCHGEIWKARSSSGAFFSKGDEVLVTELDGITLIVRGETGKELPGEE
                     KEE"
     gene            complement(2036..3373)
                     /gene="radA"
                     /locus_tag="PK849_03560"
     CDS             complement(2036..3373)
                     /gene="radA"
                     /locus_tag="PK849_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPE65233.1"
                     /translation="MAKAESVRYFCSACGASSASWAGRCPSCGEWGSLEKGTAASASG
                     VSTPQKTKSVKATDVLPPERVSSGIEEFDRVIGGGWIPGGVFLLGGQPGIGKSTLLLQ
                     TCGSMAESIGSVLYVSGEESASQIALRARRLGVLSDELHLVCTNDMAAALESLPGKKF
                     LVVDSVQAMRADGEGGWPGSPAQVRAVAQMCISAAKEHSVPAVLVGHITKEGRIAGPM
                     LLEHMVDGVLTFSGEDYSPYRMLRAGKNRFGSTEELGVFEMTETGLVPVTDVSGLYWN
                     KAAETVPGVAMTVAVEGSLPLIAEIQSLASATAFPYPKRTGRGVDVNKIQLFSAVLDK
                     RCGIGCGLFDIYVNVAGGLLLREPAADLALCAALASAVRDVPLPGDCVFLGEVGLAGE
                     IRPVVRLARRLQEAARLGFTQAIVSAREGGGGKAPLDVVRVSTLKDALKAVIS"
     gene            3544..4746
                     /locus_tag="PK849_03565"
     CDS             3544..4746
                     /locus_tag="PK849_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPE65234.1"
                     /translation="MNRLPRGCANVGGDLAAAMEECKRVFLSLFEQWGYRPFLPSGLQ
                     LLESSWERLPSLFRQKLIAVNSPYGEPCCLRGDITLATVAYLASHHSPEERPLRICYS
                     DRVYARAEKPRLAMESFQIGAELLGWERQGADAEMLFLLLRMLDEIGLEKSILLLGDA
                     TFLAKAVSEIEFSAAERIVGALQKGSLIEYAALLDEENIPDFYRAILQRIPELHGGPE
                     VLEEAAALWGRGAPLLSLKNVVSTLTALGYGDRIAIDLSLVRELGYYSGPIFDVYSEN
                     YGQSLGGGGRYDRLLSLCGLQGQAMGFGLDLQHIASLSKFRTRSPSVMAWFANLSPDE
                     ALRRAERLATGGRPLEMSWCVDRASSLENAAQRGCSSWIDLSDGSTLDLATQGDGRTL
                     PGKENFRC"
     gene            4740..5384
                     /gene="hisG"
                     /locus_tag="PK849_03570"
     CDS             4740..5384
                     /gene="hisG"
                     /locus_tag="PK849_03570"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPE65235.1"
                     /translation="MLTIALPTGRVFSEAAELLMAAGLPASSLASPGRKLVFEEENFR
                     YILAKPSDVPLYVSYGAADLAFAGGDVLQESALPLVEILDTGRGRCRVVVAGPASLAE
                     RFRGHASERMWLKVATKYPRIADNHFSSKGAQVDIIHLHGSIELAPKLGLSDCILDIV
                     QTGSTLKANHLEVLEHVSDVSLRLVASKKSVALRWADIRAVSRAIKSRCKEVRS"
     gene            5381..5947
                     /locus_tag="PK849_03575"
     CDS             5381..5947
                     /locus_tag="PK849_03575"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HPE65236.1"
                     /translation="MIEKERTTKETSVSLRLDLDGGDRRIEIPCGFLRHMLELFAFHA
                     GIGLDVTATGDVDVDCHHVTEDVGILLGKAFEQARSGRNIQRYGWCALPMDGSLVLAA
                     VDFSGRGQFEWKGAFPAERCGDFDLELLPEFWRAVCREGRITLHAQALACDNAHHLAE
                     ALFKGAGKAFRQALSPAGELQSTKGTLA"
     gene            5944..6567
                     /gene="hisH"
                     /locus_tag="PK849_03580"
     CDS             5944..6567
                     /gene="hisH"
                     /locus_tag="PK849_03580"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPE65237.1"
                     /translation="MIAVVDYQVGNTGNVRRAFERLGEQVCLLHSPDELGDRVSVAVL
                     PGVGAFGPASERLAASGWRETLQSWARSGRPLLGICLGMQLLCEKGLEDGEYEGLGLI
                     PGTALPLAASKIPHMGWNTVEWTGRPPSFLPSAPDGSFFYFVHSFALPEGEFTTGRTT
                     LEGNVFSSMVLKGSVAGFQFHPERSGPEGVALLGETVRFLRKEGAPQ"
     gene            6564..7283
                     /locus_tag="PK849_03585"
     CDS             6564..7283
                     /locus_tag="PK849_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HPE65238.1"
                     /translation="MIVFPAMDLYGGEIVRLRQGQFSDRTVYPAKIAETAKSFLEAGS
                     EWIHLIDLQGAEEGAPRHLSVVGRLKETGLLVQYGGGLRSEEDIAAAFEAGADRLYLG
                     SILVKDRFLAGRMYDAYGSKVIPAVDIRNGDVVISGWKEAGGIAPEALIERLRAIGYR
                     LFLVTAVHRDGMETGPDREMYRRLLVRFPDIGIIAAGGLSSLEDIRNLKEDGLAGAVL
                     GRSLYEGSILLKDALREGASC"
     gene            7277..>7570
                     /locus_tag="PK849_03590"
     CDS             7277..>7570
                     /locus_tag="PK849_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HPE65239.1"
                     /translation="MLTRRIIPCLDVLGGRVVKGVNFINLRDAGDPAELARTYMEEGA
                     DELVFLDITASAERRRTMLEWVSRVADTLFLPFTVGGGISAVEQAREIVALGAD"
BASE COUNT         1470 a         2484 c         2234 g         1382 t
ORIGIN      
        1 cggagctgca gcgtgatggg tgaaagctcc atctgttttg ccgcctcgct cagcttctgc
       61 gccgcctgga actcgccctc ggcgttgatg atcttcgcgc ggcgttcgcg ctccgcctcc
      121 gcctgcttcg ccatggcgcg cttcatgctc tcgggcagct ccagttcctt cagttcgacg
      181 gcgctgacct tgatgcccca tgaatcggtc cgctcatcga tgatcttctg caactcgctg
      241 ttgatttttt ccctcgacga gagcacttcg tcaagatcgg ctccgccgac gaccgaacga
      301 agtgtcgtct gcgagagaag gcttgtcgca agaatatagt tctccacctc gaccacggaa
      361 aacgcagggt ccatgacgcg gaaatagacg accgcgttga ccttgatcgg gacgttgtcc
      421 cgcgtgatga cctcctgcac cggcacgtcg agcgtcacca cgcggaggtc cactcgcacc
      481 accttgtcga tgatcgggat caccagcacg atgccgggtc ccttcgcgcc gacaaggcgt
      541 cccacccgaa agacgaccac gcgctggtac tcgggtacga tcttcaccgc ggacgccaaa
      601 atgaacagaa caatgagaat gaacccgaag gacgtcccca aactgagcaa cagctccaaa
      661 agatcgacta ccatagttta ttcctccttt tcttcgccgg gaagctcttt cccggtctct
      721 ccacgaacaa tcagcgtaat tccgtcgagc tctgtgacaa gcacctcgtc acccttcgag
      781 aaaaaggccc ccgaagagct ccgggccttc cagatctctc cgtgacaccg gaccagccct
      841 tcagggagca ggtcctccac gaccacagcc ttctttcctt tcatcccttc tctgcccgag
      901 acagccttct tattcattga acgaacaacg aggaagagcg cgccgaggaa gaagagaccg
      961 agtgcaaggc tcatgccgcc tatgacgctc atggatacgt tcaacagctc accccccgga
     1021 gcccgaaaga gaatcagccc ccctgtcaca agagaagcaa ggccgaaaag gctcagaata
     1081 ccgattccgc ccaccataag atcgaggacc agcacgccga ttcctgcaac caggaggaca
     1141 atacccgccc agttgaacgg cagcatccga agtccgaacg accccagaag caccatcacg
     1201 gcgccggccg ttcccagcac aaaccctccc ggagacatca cttcgagcac gatcatcaaa
     1261 attcccgctg taagcagcag ataggcgata tcggggcttg agatgaactg gagcgccttt
     1321 tcgcgcgacg tcataatgat ctccaccggc gttccactcc ccggggccag ttccttttcc
     1381 tcgcccttta cgagcactct tcttccgcga acgccgagaa gcagcgactc caaatcggga
     1441 gccgacagat ccgccgcccc ctcggccagc gcttcctccg ctgtgagcga aatgctatgg
     1501 gtcaccatcc gctccgccag gtccgcgttc ctctttctga gctgggcgag ggagcggatc
     1561 tgggcggcca gatcgttggt gatcttccgc ttcatttcct tgtccggcgc gtcctgtccc
     1621 gacgccatca caggatgggc ggcgccgacg ttcgttccgg gggccatcgc ggctacgtgc
     1681 gccgcttgca gaataaaggc tccggccgag gcggccctcg ctccctgcgg agagacccag
     1741 accacgacgg gaacagacga ttcgagtatc gccgcggtaa tggccctcat cgagctgacc
     1801 aggcctcccg gagtatccag gcgaaaaata acgagtccgc ctttttcctc ctctgcacgc
     1861 gtaagcaccg cggccacaaa attttccatc gacacgccca ccgttccgtc gagcgccgcg
     1921 gaaaacacct tgccttccac ggacggaacc gtaccagcct gcgcagaaaa cgccggacac
     1981 gcgcacagtg caaacagcaa aaccaggaac atggcgctct tttttaaaca gtttttcatg
     2041 atatcaccgc ctttaaagca tccttgagcg tcgaaacccg gacaacatca agaggggctt
     2101 ttccgcctcc tccttcgcgg gcgctgacga tcgcctgtgt aaaacccagc cgcgcagctt
     2161 cctgcaagcg cctggccagc ctgaccaccg gacggatctc ccccgcgagc ccgacctccc
     2221 ccagaaagac gcagtctccg ggaagcggaa cgtcccgcac cgcggacgcg agcgccgcgc
     2281 agagcgccag atccgcagcc ggctcgcgca aaagaagacc gcccgccaca tttacataga
     2341 tatcgaagag cccgcacccg ataccgcagc gcttgtcgag caccgcggaa aaaagctgta
     2401 tcttgttcac gtccactccc cgcccggtgc gcttggggta cgggaaggca gtcgcgctcg
     2461 ccagcgactg aatctcggca atgagaggaa ggcttccctc caccgcgacc gtcatcgcca
     2521 ctccgggaac cgtctccgcc gctttgttcc agtacagccc gctcacatcc gtgacaggca
     2581 cgaggccggt ctccgtcatc tcgaagacgc caagctcctc ggtactgccg aaccggtttt
     2641 tccccgcccg gagcatccgg tagggagagt agtcctctcc ggagaaggtg agcacgccgt
     2701 cgaccatgtg ctcgaggagc atcggacctg cgatccgccc ttcttttgta atatgcccga
     2761 ccagaactgc gggcacggaa tgctctttcg ccgcggagat gcacatctgc gccacggctc
     2821 gaacctgcgc cggactcccc ggccacccgc cctcgccgtc tgctctcatt gcctgtacgc
     2881 tgtccaccac aagaaacttc ttccccggga gcgattcgag cgccgctgcc atatcattgg
     2941 tgcagacgag atgcagctcg tcggagagca cccccagccg cctggcccgg agcgcgatct
     3001 gcgaggccga ttcctcgccg gagacgtaga ggacggaacc gatactctcc gccattgagc
     3061 cgcacgtctg gagcagcagc gtcgattttc cgatgccggg ctgtccgccg agcaagaaga
     3121 cgccccccgg aatccatccg ccgccgatga cgcgatcgaa ctcctcgatc ccggaagaaa
     3181 cacgctccgg cggaagcaca tccgtagcct tgacgctctt ggtcttctgc ggtgtactca
     3241 cgccggatgc tgaagcggca gttccttttt caagcgaccc ccactcgccg caggagggac
     3301 atcttcccgc ccacgaagcg gaggacgccc cgcaggcaga gcagaaatac cgtacggatt
     3361 cagccttcgc catacctctc gcctcccagc tcagtgtatc tctcccgctt tctcctgtcg
     3421 agaggcttta cacttttaca atacaatgat atcatgccgt gagtattaaa gacattaagg
     3481 tgataatcta cccagctaaa gtgaggaaat gacaatgaca atacaaatca agagggtaca
     3541 catatgaacc gccttccgcg cggctgcgcc aacgtcgggg gggatcttgc ggcggcaatg
     3601 gaagagtgca aacgggtctt tctttcgctc ttcgagcaat ggggctaccg cccgttcctg
     3661 ccttcgggtc ttcagcttct cgaatcttca tgggagcggc tgccgtcgct cttccgacaa
     3721 aaactcatcg cggtgaactc cccgtacggc gagccgtgct gcctccgcgg agacatcacg
     3781 ctggcgaccg tcgcctacct cgcctcgcac cactcgcccg aggagcgtcc gctgcgaatc
     3841 tgctactcgg acagggtgta cgcgcgggcg gaaaaacccc gccttgcgat ggagtcgttc
     3901 cagatcggcg cggagctgct cgggtgggaa cggcagggcg cggacgcgga gatgctcttt
     3961 cttcttctcc ggatgctcga cgaaatcgga ctggagaaga gcattctcct cctcggcgac
     4021 gcaacgtttc tggcaaaggc ggtctccgag atcgaatttt cggccgccga gaggatcgtc
     4081 ggcgcccttc agaaaggatc gctgatagaa tacgccgccc tcctcgacga ggagaacatc
     4141 cccgacttct acagggctat tctccaacgt attcccgagc ttcacggcgg tccggaggtg
     4201 ctcgaagaag cggcggcgct ctggggaaga ggcgctcctc tcctctcgct caagaacgtc
     4261 gtctccacgc tgacggcgct cggctacggc gaccgtatcg ccatcgacct gtcgctcgtc
     4321 cgcgaactcg gctactacag cggaccgatc ttcgacgtct attccgaaaa ctacggacag
     4381 tctctcggag gcggagggag gtacgaccgg ctgctctcgc tctgcggact ccaggggcag
     4441 gcgatggggt tcggactgga tctccagcat atcgcgtcgc tctcaaagtt tcgcacgcgt
     4501 tccccttccg tcatggcctg gttcgcgaat ctctcgccgg acgaggcgtt gcggcgggct
     4561 gaacgcctgg cgacgggagg gaggccgctc gaaatgagct ggtgtgtcga ccgggcttcc
     4621 tccctcgaaa atgccgcgca gcgggggtgc tcctcctgga tcgatctctc ggacggctcg
     4681 acgcttgatc ttgcaacgca aggggatggc cggacgctcc ccggaaagga gaatttccga
     4741 tgctgaccat cgccctgccc acaggccgcg tcttctccga agcggcggag ctgctcatgg
     4801 ccgccggact gccggcgtcg tcccttgcct cgccgggacg caagctcgtc tttgaagagg
     4861 aaaacttccg ctacatcctg gcgaaaccct cggacgtgcc gctctacgtg agctacgggg
     4921 cagccgacct cgccttcgca ggaggcgacg ttctccagga gagcgcgctg ccgctcgttg
     4981 aaatcctcga cacggggaga gggcgctgcc gcgtcgtcgt cgccgggccc gcctcgcttg
     5041 ccgagagatt ccgggggcac gcctccgagc ggatgtggct caaggtggcc accaagtacc
     5101 cgcgcatcgc ggacaaccac ttctcctcca agggcgcgca ggtggacatc atccacctcc
     5161 acggctccat agaactggcg ccgaagcttg gactgagcga ttgcatcctt gacattgtcc
     5221 agaccggcag tacactgaag gccaaccacc tcgaagtgct ggagcacgtc tccgacgtct
     5281 cgctccgcct ggtggcgagc aagaagagcg tcgccctccg ctgggccgat atccgggcgg
     5341 tgagcagggc gatcaaaagc cggtgcaagg aggttcggtc atgatcgaga aagaacggac
     5401 gacaaaggag acctcggtct ctctgcgctt ggatcttgac ggaggagacc ggcgcatcga
     5461 gattccgtgc ggctttctgc gccacatgct ggagctcttc gcctttcacg ccgggatcgg
     5521 cctcgacgtg acggcgaccg gagacgtcga cgtggactgc caccacgtga cggaggacgt
     5581 cggtatcctg ctgggaaagg cgttcgaaca ggcccgttcc ggccggaaca ttcagcgcta
     5641 cggctggtgc gctcttccca tggacggctc tcttgtgctg gccgccgtcg atttcagcgg
     5701 ccgggggcag ttcgaatgga agggagcgtt ccctgccgag cggtgcggcg acttcgacct
     5761 tgagcttctg cccgagttct ggcgcgcggt ctgccgcgaa ggaaggatca cgctccacgc
     5821 gcaggccctc gcctgcgaca acgcacacca tcttgcagag gcgctcttca agggagcagg
     5881 caaggcgttc cggcaggccc tctctcccgc cggagagctg caaagcacga aaggaacgct
     5941 cgcatgatcg ccgtcgtgga ctaccaggtg ggaaacacgg gtaatgtccg ccgcgccttt
     6001 gagcgcctcg gggagcaggt ctgtctgctc cactctccgg acgaactcgg cgaccgcgtt
     6061 tcggtcgccg tgcttccggg agtcggggca ttcggaccgg cttcggaacg tctcgcagcc
     6121 tcggggtggc gggaaacact tcaatcgtgg gcgcgttccg ggcggccgct gctcggcatc
     6181 tgcctcggga tgcagcttct gtgcgaaaag gggcttgagg acggggaata tgaaggcctc
     6241 ggactgatcc cgggaaccgc gctccccctt gcagcgtcca aaattcctca catggggtgg
     6301 aacactgtag aatggaccgg tcgaccgccg tcgtttttac cgtccgcgcc tgacggctcg
     6361 ttcttctact tcgtccatag cttcgcgctc cccgaaggtg aattcacgac agggcggaca
     6421 acgctcgaag gaaatgtctt ctcgtcgatg gtgctgaagg gctccgtggc gggattccag
     6481 tttcacccgg agcgcagcgg ccccgaaggc gtcgcgctgc tgggggagac cgtcaggttt
     6541 ctacgaaagg agggcgcccc gcaatgatcg tttttcccgc gatggatctc tacggaggcg
     6601 aaatcgtccg cctcaggcag ggacagttca gcgacagaac cgtgtatccg gcaaagatcg
     6661 ccgagacggc gaagagcttc cttgaagcag ggtcggaatg gattcacctg atcgacctgc
     6721 aaggcgccga ggagggagcg ccgcgccacc tttccgttgt cggccgtctc aaggaaacag
     6781 ggctccttgt tcagtacggc ggaggactcc gatccgagga ggacatcgcc gcagccttcg
     6841 aggcgggcgc cgacaggctc tatctgggaa gcatcctcgt gaaggatcgt ttcctcgcgg
     6901 gcaggatgta cgacgcctac gggtcgaagg tcatccccgc ggtagacatc cgcaacggcg
     6961 atgtggtgat ctccggctgg aaagaggcgg gcggcatcgc cccggaagcg ctgatcgaaa
     7021 ggctgcgggc gataggatac cgtcttttcc tcgtcaccgc ggtgcaccgt gacggcatgg
     7081 agacggggcc ggacagggag atgtaccgcc gccttctcgt ccgctttccg gatatcggaa
     7141 tcatcgccgc aggggggctc tcttcgctgg aggacatacg aaatctgaag gaggacgggc
     7201 tcgcgggcgc cgtcctcggc agatcgctct acgaaggaag catcctcctg aaggacgcgc
     7261 tccgagaggg cgcttcatgc tgacacggcg aatcatcccg tgcctcgacg tgctcggggg
     7321 gcgcgtggtg aagggcgtga acttcatcaa cctgcgcgac gcgggagacc ctgcggagct
     7381 ggcgcgcacc tatatggagg agggcgccga cgaactggtc ttcctcgaca tcacggcctc
     7441 cgccgaacgc aggcggacga tgctcgaatg ggtctcgcgg gtagcggaca cgctcttcct
     7501 ccccttcacc gtcggaggcg gcatctccgc tgtggagcag gcccgggaga tcgtcgcgct
     7561 cggagcggac
//
LOCUS       DAOPKU010000076         7567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10380_length_7567_cov_4.821219, whole genome shotgun sequence.
ACCESSION   DAOPKU010000076 DAOPKU010000000
VERSION     DAOPKU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10380_length_7567_cov_4.821219"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2048
                     /gene="pbpC"
                     /locus_tag="PK849_03595"
     CDS             <1..2048
                     /gene="pbpC"
                     /locus_tag="PK849_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPE65240.1"
                     /translation="EKMGRWLPFVAVEVEDRRFMEHGGVDYTALLRAVWQNISAGKVI
                     SGASTITSQLVRLSFPRPRTLKTKLVEFTAAQKLEEMLTKEEILELYLNRAPFGGNIR
                     GVEAASRIYFSKGAGEVSLGEAALLVGMLKGPSLYRPDRNPGAARKRRDAILASLFER
                     GKITEEQYRLSRAERIPDTRGAFPSKAFHFALAALGERKKGGVLETTLDPALQELLER
                     TLSVSLESLPEEITAAGIVMDNDSGDVLAYVGNGRLGRGIAGSWIDCARRPRSPGSAL
                     KPFAYLAAFEKGILTPASLLADTPLAFSGQAPRNFDLTYRGPVSARIALADSLNVPAV
                     RVLRAAGPELVLDLLRNAGFLSLTQSTAHYGDSLILGGCETSVLQMAEGYVMLATLGI
                     RRSPRFLLSEPPFHRRLVSEGAAFLVADILKDTGRLLPLHGKRIEYGKEWFAFKTGTS
                     YGYRDAWTAAYTPRNTVVVWMGNPTGKAHPELVGSQAAAPAIVEILRGLPPSGWYDAP
                     QQVIMRTVCALSGRPIAPACPVGRLDYALEGISSSVPCPMHVIRDGSARTVWPAELEE
                     FALRRSLTLQAEQKATITSPLPGSRYILTPLGGDQRVALKAEGAKPPVYWFVGGEYAG
                     KQEGKEALFWTLKKGVHAVSLVDAAGRTAVSRFTVDAPGVKGPDESGPPPLLLNPGD"
     gene            complement(2032..2982)
                     /locus_tag="PK849_03600"
     CDS             complement(2032..2982)
                     /locus_tag="PK849_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPE65241.1"
                     /translation="MKGTVVGMAVAYKDYYEILGVSRTASQDEIRKAYRKLAKEHHPD
                     VNKNNGSDERYKEINEAYEVLKDPEKRQKYDTLGPNWQQGQDFTPPPGWGGGVHMDFG
                     GDGGGFSDFFRTIFGGFASGGRGVSMDDLFRSQGRGGDEEMSLELSLEDILRGGTKTI
                     SMEQVVHGADGRPSRSRRTLNVNLPQGVTEGSRIRLKGQGSRGADLYIVIHAAPHPKF
                     TVEGYDLVTSLKVSPWEAALGASIPVTTLDGVVSMKLPAGVQAGKRLRLKEKGLPRRK
                     GAQAGDLIVKVEVVVPEKLSDRERELFEALAKESSFNPRG"
     gene            complement(3111..3923)
                     /locus_tag="PK849_03605"
     CDS             complement(3111..3923)
                     /locus_tag="PK849_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HPE65242.1"
                     /translation="MDIGISKRLAKLYEEGKEAVLCMVVEESGSTPRSMGSSMLVFPG
                     GKIEGTVGGGITEHRVIEKALELLGKGTGSMMYRETLSATEAALDGAACGGSMGIYLE
                     VIGRKRELVIFGAGHVGKAIARLGDMTGFAVTIWDERAEFANEENIPWGRTVCCPLEN
                     FFDHVAPFHGDTYVIIVTRGHVLDADVVKLVEGMTAAYMGMIGSRKKIAFVRERLLGQ
                     GVSAGHIDRIYQPVGLPIRAETPEEIAVSAMAEIIAVARGADLPALRSSLTQ"
     gene            complement(3923..5029)
                     /gene="mnmA"
                     /locus_tag="PK849_03610"
     CDS             complement(3923..5029)
                     /gene="mnmA"
                     /locus_tag="PK849_03610"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPE65243.1"
                     /translation="MNIRPSTGFSSSSPSFGQGTVVVLMSGGVDSSVTAWLLQKAGWH
                     VVGVTMKIPGADLSPGSCCGRDAASVALGLGIPHYTAFIEREFEQLVIEPFVESYLRG
                     STPNPCADCNALLKFGLLWDELEDAFGETRLATGHYARVEQEGERFFLSRGKDPSKDQ
                     SYFLYGVARRRLPRLLLPLGGYSKEDVRALAREAALPVAEKSESMEICFAGEGDYRRL
                     LDGRSLPPGLIVDEDGEPLGAHRGIANYTVGQRKGLGISSRDGLHVLRIDPGRNAVIV
                     GPRERAFRRRVRASNVNILFPEPPAKGALLFGKTRSRGEPLPCDLLRMDENVLEVLFH
                     EPQFAPTPGQRLVLYDAGGRVAAGGVIEYSEEAE"
     gene            5118..5948
                     /gene="larE"
                     /locus_tag="PK849_03615"
     CDS             5118..5948
                     /gene="larE"
                     /locus_tag="PK849_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPE65244.1"
                     /translation="MPYHAAMKKMERVEALLREMGSAAVAFSGGVDSTLLAAAASRAL
                     GERAVAVTAASPTLPSWEIDDAEQYAALIGIRHVSIPLSELEEPRFAANDREKCYYCK
                     RFRFELLLKWAKKEGFRWVLDGSNTDDLNDYRPGMRALSELEGVRSPLLEAGLSKSEI
                     RAVSKQWGLPSWQKPAAACLASRIAWGVPITAERLRQVERAEEIVRSFCPPNTQIRVR
                     HHGALARIETPPSALSLLAAPETAARLAPAVLALGFSFVTLDLSGYKMGSLNALFTDG
                     "
     gene            complement(5931..6497)
                     /locus_tag="PK849_03620"
     CDS             complement(5931..6497)
                     /locus_tag="PK849_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPE65245.1"
                     /translation="MRRIFDGYARFFSSVDLDEYRALAGGQRPHSLVITCSDSRIVPE
                     ELFDARPGELFVLRNVGNLARLDEPSVAAALDYALLHLGVKNVVVLAHTDCGAVKASK
                     NVDHLDTDGLKRWLGKEFFEGADSEEAGKAAGLRQLRRVEQYPLFKKMADEGSVSASL
                     LFFHIDTCALEVYEDGVWSVPERLAVSK"
     gene            complement(6738..7205)
                     /gene="msrB"
                     /locus_tag="PK849_03625"
     CDS             complement(6738..7205)
                     /gene="msrB"
                     /locus_tag="PK849_03625"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPE65246.1"
                     /translation="MKLTLFVLCACTLLLFAVRAQATAPERVEKSEEEWRAILTPEQF
                     EITRKKGTELAFSGAYWDHHEKGVYACVCCGAQLFSSEAKYDSGSGWPSYWMTIEGAP
                     VAEKRDVSFGMIRTEVLCARCDAHLGHLFTDGPPPTGLRYCINSASLTFIPAE"
     gene            complement(7219..7494)
                     /locus_tag="PK849_03630"
     CDS             complement(7219..7494)
                     /locus_tag="PK849_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65247.1"
                     /translation="MEHISSIITDFIVKNMNERGLSLYRTDEEKILALDDQYETCFKF
                     DLVLSDNDFSCAVLSQGEHGLVLRRRFNIPWTNAAEIREFMEFVRSL"
BASE COUNT         1473 a         2413 c         2190 g         1491 t
ORIGIN      
        1 tcgaaaagat gggacgatgg cttccgttcg tcgccgtcga ggtagaggac cggcgtttca
       61 tggagcacgg cggggtcgac tataccgcgc tcctccgcgc cgtatggcaa aacatctcgg
      121 caggcaaggt aatttcggga gcgtcgacga tcacaagtca gctcgtccgc ctctcttttc
      181 cgcgcccgcg gacgctgaag acgaagctgg tggaattcac cgccgcgcag aagctcgagg
      241 agatgctcac gaaggaggag atactggaac tctatctgaa ccgcgctccg ttcggcggca
      301 acatacgggg cgtcgaagcc gcctccagaa tttacttctc gaagggcgcc ggggaggtct
      361 ccctcggaga ggcggcgctc ctggtgggaa tgctcaaggg gccgagcctc taccgtcccg
      421 accggaaccc cggggccgcg agaaaacggc gcgacgccat actcgcctcg cttttcgaac
      481 ggggaaagat cacggaagag caatatcgcc tttcacgagc cgaacggatt cccgatacgc
      541 ggggggcttt cccgtcgaag gcgtttcatt tcgccctcgc cgcgcttgga gaacgaaaaa
      601 agggaggcgt actggagacg acgctcgacc ccgcgcttca ggagcttctt gagaggacgc
      661 tgtccgtctc cctggagtcc ctgcccgagg agatcaccgc cgccggaatc gtgatggaca
      721 acgattcggg cgatgttttg gcctatgtgg gcaacggacg ccttggaaga gggatcgccg
      781 gaagctggat cgactgcgcc cgccgccctc ggtcccccgg ctccgcgctc aagccgttcg
      841 cctatcttgc agcattcgag aagggaattc tcacccccgc gtcgctcctg gcggacactc
      901 cgctcgcctt ctccggacag gcgccgcgga acttcgacct gacctacagg ggaccggttt
      961 cagccaggat agccctcgcg gactcgctca acgttccggc agtcagggta cttcgggcgg
     1021 cggggccgga gctggtgctc gacctccttc gcaacgcggg tttcctgagc ctgacgcaga
     1081 gcacggccca ttatggagat tcgctgattc ttggagggtg cgaaacgagc gttcttcaga
     1141 tggcggaggg gtacgtcatg ctggcgacgc tggggatacg ccgatcgccg cgttttctcc
     1201 tgagcgaacc tccatttcac aggaggctgg tctccgaagg agcggcgttt ctggtcgccg
     1261 atatcctcaa agacactgga cgactcctcc ccctgcacgg caagcggata gagtacggga
     1321 aagagtggtt cgccttcaaa acaggtacct cgtacggcta ccgcgacgcc tggaccgccg
     1381 cctatacgcc gcgcaacacc gtcgtcgtct ggatgggcaa tcccacaggg aaagcgcacc
     1441 cggagctcgt tggctcgcag gccgccgctc ccgccatcgt cgagatcctc cgcggcctcc
     1501 ctccctcggg gtggtacgac gcgccgcaac aggttatcat gcgcacggtg tgcgctctat
     1561 cgggccggcc gatcgctccc gcctgccctg taggaaggtt ggactacgct cttgagggta
     1621 tttcgtcctc cgtgccgtgc ccgatgcacg tcatcagaga cggatcggca cggacagtct
     1681 ggcccgccga actggaagag tttgctctgc gccgttcgct gacgctccag gcggagcaga
     1741 aagcgacgat cacatctccg ctccccggct cgcggtatat tctcacaccg ctcggagggg
     1801 atcagagggt ggcgttgaag gccgagggag ccaagccccc ggtctattgg ttcgtgggag
     1861 gagaatatgc cggaaaacag gagggaaagg aagcgctctt ctggacgctc aagaaagggg
     1921 tacacgccgt ttcgctggtg gacgccgccg gaaggaccgc cgtttcgcgg tttaccgtcg
     1981 acgctccggg cgtgaaggga ccggacgaat ccggccctcc gccgcttctt ctcaaccccg
     2041 gggattgaac gacgactctt ttgcgagcgc ttcgaacaac tcccgctcgc ggtcgctcag
     2101 tttctcgggc acgacgacct cgactttcac gataagatcg cccgcttggg ctccctttct
     2161 tctggggagc cctttttctt tcaaacggag ccgctttccc gcctgaactc ctgccggaag
     2221 cttcatcgag accacgccgt cgagcgtcgt cacggggatg gatgctccaa gggcggcctc
     2281 ccatggagag accttcaggg aagtgacaag atcgtacccc tcgacggtga acttgggatg
     2341 gggcgccgca tggatgacga tgtacagatc ggcgcctctg cttccctgcc ctttgagcct
     2401 gatgcgcgaa ccctcggtca cgccctgggg aaggttcacg ttgagcgtcc tccggcttcg
     2461 ggacggacgt ccgtccgcgc cgtgcaccac ctgctccatc gaaatggtct tcgtccctcc
     2521 ccggagaata tcctcaagcg agagttcgag agacatctcc tcgtcgccgc cacgcccctg
     2581 cgaacggaag agatcatcca tcgaaacgcc tctgccgccc gaggcgaagc cgccgaagat
     2641 cgttctgaag aagtcgctga acccgccccc gtcgccgccg aaatccatgt gcacgccccc
     2701 gccccatccg ggaggcggcg taaaatcctg cccctgctgc cagttcggac caagagtgtc
     2761 gtacttctgc cgtttctcag gatccttgag cacctcgtag gcctcgttga tctccttgta
     2821 gcgttcgtcg gagccgttat tcttgtttac atccgggtga tgctccttcg cgagcttccg
     2881 gtacgccttc cggatctcgt cctgggacgc cgtccggctc acgccgagaa tctcgtaata
     2941 gtccttgtat gcgacggcca tgcccaccac cgtgcctttc ataccgaata aattctaacc
     3001 aatactgacc tttatagtgt acactttttt ttagcgttct caataggcgc gaatatcaaa
     3061 taggcgctga aaatgccgat gctcgctctt ttccttttat tttctctgtt ttactgtgta
     3121 agagatgagc gcagagcggg cagatccgcc cctctggcca cggcgatgat ctccgccata
     3181 gcggaaaccg ctatttcctc cggcgtttcc gcacgtatcg gcaagcctac cggctggtaa
     3241 atccggtcga tgtgcccggc ggagactccc tgtcccaaca gccgctcgcg cacgaaggcg
     3301 atcttctttc tggaaccgat catccccatg tacgcggcag tcattccttc gaccagcttc
     3361 acgacgtccg cgtcaaggac atgccctcgc gtaacgatga tgacgtaggt gtccccgtgg
     3421 aaaggggcga catgatcgaa aaaattctca agcggacagc agacggtcct tccccacggg
     3481 atgttctcct cgttggcgaa ctccgctctt tcatcccaga tggtgacggc aaatccggtc
     3541 atgtccccga gacgggcgat cgctttcccg acgtgccctg ccccgaagat gacgagttcg
     3601 cgctttctcc cgatgacttc gaggtatatt cccatggaac ctccgcaggc cgccccgtcg
     3661 agcgcggcct cggtagcgga gagcgtctct ctgtacatca tgctgcctgt tcctttgccg
     3721 agaagttcga gggctttttc gatgacgcgg tgctcagtga taccgccgcc gactgtccct
     3781 tctatctttc ccccgggaaa gacgagcatc gaggatccca tgcttcgggg agtcgacccg
     3841 ctctcctcca ccaccataca gagcaccgct tcttttcctt cttcgtagag cttcgccagc
     3901 ctcttcgaaa tgccgatatc cattattccg cctcctcgct gtattcaatg acgccccccg
     3961 cggccactct gccgccggcg tcgtagagta ccagccgctg ccccggagtc ggggcgaact
     4021 gcggctcgtg aaataagacc tcaagcacgt tctcgtccat gcggagcaga tcgcacggaa
     4081 gcggctcccc ccgcgagcgg gtcttgccga aaaggagcgc ccctttcgcc ggcggttcgg
     4141 ggaagaggat attcacattg ctcgcgcgta cgcgccgcct gaaagccctc tcccgaggac
     4201 cgacgatgac ggcgtttcgc ccgggatcga tccgcagcac gtgcagtccg tcccgcgagg
     4261 agattccaag cccttttctc tgccctacag tgtagtttgc tatccccctg tgcgcgccga
     4321 gtggttcgcc gtcctcgtcc actatcagtc ccggcggaag cgaacggccg tcgagcagtc
     4381 ggcggtaatc cccctcgccc gcgaaacaga tctccatact ctcgctcttc tcggccacag
     4441 gaagggcggc ctccctcgcg agagcgcgca catcctcctt ggaataccct cccagcggaa
     4501 gcagaagtcg cggaaggcgg cggcgcgcca ctccatagag gaaatagctc tgatccttgg
     4561 aagggtcctt tcctctggag agaaagaagc gctctccctc ctgttctacg cgcgcatagt
     4621 gcccggtggc gaggcgcgtc tctccgaacg catcctccag ctcgtcccac aggagaccga
     4681 acttcagcag cgcattgcag tcggcgcatg ggttgggagt gctcccgcga aggtagctct
     4741 ccacgaacgg ttcgatgacg agctgctcaa actctctttc gataaaggcc gtatagtgcg
     4801 gaattccgag cccgagggcc accgacgccg catcgcgtcc gcagcagctt ccgggcgaca
     4861 aatccgctcc cgggatcttc atggtgacac ccacgacatg ccacccggct ttttgcagca
     4921 gccaggccgt cacggagctg tccacgccgc cgctcatcag gacgacgact gtgccctggc
     4981 cgaaggaggg cgatgaggag ctgaatccgg tcgatggtcg aatgttcatg aggaacttcc
     5041 ctccgggcat tggaatgtct tctcttctca tgtagtatag agaagaacaa agttcttcac
     5101 aagggaggat cgagagcatg ccgtaccacg ccgcgatgaa aaaaatggag agagtggagg
     5161 cattattacg ggagatggga agcgcagccg tggcgttttc gggcggagtg gacagcacgc
     5221 tcctcgccgc agcggcttct cgcgcccttg gagagagggc cgtggccgtc accgccgcgt
     5281 cgccgacgct tccttcatgg gagatcgacg acgcagagca gtacgccgct ttgatcggca
     5341 tccgccacgt gagcattccg ctctcggaac tggaggaacc gcgctttgcc gcaaacgaca
     5401 gggagaagtg ctactactgc aagcgattcc gtttcgagct gctgctcaag tgggcgaaaa
     5461 aagaggggtt ccgctgggtg ctcgacggct ccaacaccga cgacctgaac gactaccgtc
     5521 cgggaatgcg cgccctctcc gagcttgagg gggtacggag cccgcttctc gaagccggat
     5581 tgagcaaatc ggagatacgc gccgtctcaa aacagtgggg gcttccgtcg tggcagaaac
     5641 ccgcggcagc ctgccttgcg agccggatcg catggggcgt gcccatcacc gcggagcgct
     5701 tacgccaggt agaacgggcg gaagagattg tacgctcctt ttgccccccc aatacgcaaa
     5761 tcagggtccg tcaccacgga gcgctggcga gaatcgaaac tccgccctcc gcgctctcgc
     5821 tgctcgccgc gccggagact gcggcacggc tcgcgcccgc tgtgctcgcc ctcggctttt
     5881 ctttcgtcac cctcgatctc tccggctata aaatggggag tctgaacgcg ttatttactg
     5941 acggctaacc gctcgggaac gctccatacc ccgtcttcgt acacttcaag agcgcaggtg
     6001 tcgatgtgga aaaacaagag ggaggccgat acggagcctt cgtcggccat cttcttgaaa
     6061 agcggatact gctcaacgcg gcgaagctgg cggagcccgg cggctttccc ggcttcctcg
     6121 gagtcggcgc cctcgaagaa ttctttcccg agccagcgct tcagcccgtc cgtatccaga
     6181 tggtcgacgt ttttcgaggc cttcaccgct ccgcagtccg tatgcgcaag caccacgaca
     6241 ttcttcactc cgaggtgaag gagcgcgtag tcgagagccg cagcgaccga gggttcgtca
     6301 agcctggcaa ggttcccgac gttccgcaga acaaagagct ctccggggcg ggcgtcgaac
     6361 agctcctccg ggacgatccg gctgtccgaa caggtgatga cgagcgaatg aggacgctgt
     6421 cctccggcga gcgcacggta ctcgtcgagg tcgacagacg aaaaaaagcg ggcatatcca
     6481 tcaaaaatcc tgcgcatggc atacaaactc cctttcacat ggaatcgatt gaggcgaaca
     6541 ggcgtgcgtt gcgggcgtgt tcatccaatg gagcattatg ctcccgaaaa gaacacattt
     6601 caagcccctt tgtccagccc gcgcgtgcat gaagaagcgc tgttctccgc caaccggggc
     6661 aagaagcgtt ccctgcaaat ggacagccgt ccgcatcctg acgaagcgga cggctgcaga
     6721 agcaataccg gagcagatca ctccgcagga ataaaggtca gcgaagcgga gttgatacag
     6781 tagcgaagcc ccgtaggggg cggaccatcc gtgaacaggt gccccaggtg cgcgtcgcac
     6841 cgcgcgcaaa gaacttccgt gcggatcatc ccgaaggaga catcgcgctt ctccgcgacg
     6901 ggcgctccct cgatcgtcat ccagtagctc ggccatccgg agccggaatc atacttggcc
     6961 tccgaggaaa agagctgcgc gccgcaacag acgcaggcat ataccccttt ctcatgatga
     7021 tcccagtacg cccctgaaaa tgcgagctcc gtcccctttt ttctggtgat ctcgaactgc
     7081 tccggggtga ggattgcccg ccattcctcc tcggacttct ccaccctctc cggagcggtc
     7141 gcctgcgcac ggacggcgaa aagaagcagc gtacaggcgc agagaacgaa aagtgtcagt
     7201 ttcatgagtt tccctccttt acaggctgcg gacgaactcc ataaactccc tgatttccgc
     7261 ggcgttcgtc catgggatgt tgaaacgtct gcgcaacacg agcccgtgct ccccctgcga
     7321 gagcaccgcg cagctgaagt cgttgtccga gaggacaagg tcgaacttga aacatgtctc
     7381 gtactgatcg tcgagcgcca agattttctc ctcgtcggtt ctgtagagtg ataagcctct
     7441 ctcgttcatg tttttcacga tgaaatcagt tatgatactg gaaatatgct ccactgcaca
     7501 catcctccct tccccatgac ctttttgcgc tcagagcgta caatagtata aatcaccccg
     7561 gtgcgag
//
LOCUS       DAOPKU010000077         7537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10436_length_7537_cov_4.311949, whole genome shotgun sequence.
ACCESSION   DAOPKU010000077 DAOPKU010000000
VERSION     DAOPKU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10436_length_7537_cov_4.311949"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..487)
                     /locus_tag="PK849_03635"
     CDS             complement(26..487)
                     /locus_tag="PK849_03635"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HPE65248.1"
                     /translation="MDELDLRLIAELQENLPLTVAPYRTLAERLGVSEETVIARLQAL
                     RASGKLKRIGAVLRHQNSGYAANAMVVFKAPPDEMERLGKLLAESPLVSHCYERAPCA
                     EWPYTLYAMMHGRNMETIEEFVSIFALEHGVERFDILRSEEELKKSSLSFF"
     gene            complement(493..966)
                     /locus_tag="PK849_03640"
     CDS             complement(493..966)
                     /locus_tag="PK849_03640"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65249.1"
                     /translation="MLQERNMDNADRKLINLLQEGLPLERDPYGVFAAKLGVSREEIV
                     ERTGALMREGIIRRLGGVFDSGAMGYSSMLVGAHVPEDIFGDAARFVNAHPGVTHNYR
                     RSGFLNMWFTLSTVCDEERDAFLEELRRRFALSQVFPFPRLRSFKLRVFFDMEGA"
     gene            complement(924..1937)
                     /locus_tag="PK849_03645"
     CDS             complement(924..1937)
                     /locus_tag="PK849_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE65250.1"
                     /translation="MLISWNTTRRCNLFCRHCYRESGPGEDIQGELSTAEGIELIASI
                     AEAEFRLLIFSGGEPLLREDILELIAAARDRGLVPAMGTNGTLLTESVARELAAAGLR
                     GIAISVDSLDREYHDAFRGSHGAYDLTQAGIRNALAAGLRVQINLTLTEKNLEEFDKM
                     VAYYESTGVHAIHPFFLVPTGRARSMPEEELKSGAYFGMIRKILERQATTTLELKPTC
                     APQFMPMAKEMGIAQRFTRGCLAGVSYCCILPKGEVHVCPYLPVEAGNVRRTAFADIW
                     RESEVFAELRDFSRYEGRCGACEYVAICGGCRARAFSYTGNYMAEEPWCFRSGTWTTP
                     TGS"
     gene            complement(1931..3109)
                     /locus_tag="PK849_03650"
     CDS             complement(1931..3109)
                     /locus_tag="PK849_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE65251.1"
                     /translation="MISVTKLLLGREYSGDRLRYRRGTNSAGAADGLGPVVVWNCTRA
                     CNLLCRHCYASATPYACDAELSTGEAKDFLDTLAAFNVPALLLSGGEPLLRRDLFELL
                     EYARTLGLRTALSTNGTLIDERTAARIMELGVSYAGISLDGSRSVNDAFRGVDGAYDR
                     TISGFRNCRKAGQKAGLRLSMTRSTLKELPEIFRLVEDEGIARVCLYHLVPSGRGAEL
                     TDEEPSPEEKRAALDFLAEKTLDFAARGVETEILTVDNHCDGIYLYLREKERGGSRAA
                     EMLELLTASGGNRSGVAIGAVDWEGDVHIDQFTQEIILGNVRERDFPELWRGETDSFL
                     HRLRDRKRHLRGRCRTCGWLEQCNGNLRARALAAGDFWGPDPSCYLTGDEIASGGATC
                     "
     gene            3390..3920
                     /locus_tag="PK849_03655"
     CDS             3390..3920
                     /locus_tag="PK849_03655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPE65252.1"
                     /translation="MRYTTALPGLIIRDAVEQDVPLILSFIRELAVYEKMLDDVVATE
                     DGLKEHLFGERRVAEVLIAEWEGAPVAFALFFHNFSTFLGRPGIYLEDLYVKPEMRGK
                     GIGKAMLSHIANIAKKRGCGRFEWSVLDWNEPAITFYRSIGAVAMDEWEHFRLSGEAL
                     ETVAEMCAPENVYPKQ"
     gene            complement(3995..5611)
                     /locus_tag="PK849_03660"
     CDS             complement(3995..5611)
                     /locus_tag="PK849_03660"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HPE65253.1"
                     /translation="MALRIETRGFDLVLRNARIVDGSNSPWYRGDVGVRDGSVVFVGT
                     LPPDTETATEIDAAERVLAPGFIDMHTHNDFLLLKEPASVPKLKQGVTTVMVGQCGIS
                     AAPITPEKVALLESYTGFIMAGALPLWNWRSFGEYLDVLGGMELGVNVGSFVGHGTIR
                     LNVLGFEDRTPSSGELAAMRGHARRAMEEGAFGMTSGLVYAPGVYSAPEEVREIAKGL
                     VERRGLYLSHMRSESDDVVASVRETIAVAENLGIPGQVLHHKACGRRNRGRVRETMRL
                     LEEARERGVDMTADQYPYAAGSTTLRSILPPWIHEGGVEKLIERLSGPALRERLKREI
                     PEAEGWENMLRNSGGPEGVMMLYCPKTPDFERKRLTEIGAMTGKDPLDAAFDIIIENR
                     GSDTACFFQLAEEDVQFVMKHPLVMVASDSIPPAPGAKCHPRTNGTFPRVLGKYVREE
                     KTLRLEEAVWKMSGFPAARLGLSKKGLIREGMDADLVLFDPETVVDGATFEDPFGEPK
                     GISHVFVAGRLAVKNGEYTGAAAGKVLRKV"
     gene            complement(5614..6471)
                     /locus_tag="PK849_03665"
     CDS             complement(5614..6471)
                     /locus_tag="PK849_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPE65254.1"
                     /translation="MFRKTVARSFWVAALIAVVCAGAAVAMDVEQAADKVYLAAVEGT
                     PFPVLSAEYADMTEAGSYAVQKAYSLKRLEGKRPAGFKAGLTTEATMKRFGASTPFAA
                     PLFPEGAMDGSAESVVVDRASFGVLMLEAEIAFILGEPVDASLKDEAELKAKIESVAA
                     AVELPDLSFTDMKLLKAMDINASAISSKAWILGKRIPLADAPDLNELVPVMTLDGVEA
                     TRGRGSDALGDQWKALLWLVNKMVEEGWTMEKGDVLLTGALGNMIPGKPGEYVYSIEP
                     LGTVKFTVK"
     gene            6652..7044
                     /locus_tag="PK849_03670"
     CDS             6652..7044
                     /locus_tag="PK849_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65255.1"
                     /translation="MKRILVVDDSAVSRKLALFVLKNLGCEARGVPSAAEAFEALRNE
                     VYDAIFMDLHMPEMNGCEASMFIRRSGAGEANRSLPIFAITGSDLPEERESCVSAGMT
                     DFLAKPFSGEHAEALLRKHCGKTAHDGQ"
     gene            7103..>7537
                     /locus_tag="PK849_03675"
     CDS             7103..>7537
                     /locus_tag="PK849_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65256.1"
                     /translation="MSGISAVPPVNSIKTVVPVANRTRREGLQRAEREVMRQELAVRN
                     QGDVTSTVYHYEIGPDGRRYITGATVTYKEKENELSSKSAKDKSSASSANTGPASSAE
                     NGRVELTDESRAKLKAIERDVIAHEAAHKAVGGQYAGPVSYTY"
BASE COUNT         1507 a         2439 c         2149 g         1442 t
ORIGIN      
        1 atcccggaac tggcgaaggt tctttttaga aaaacgagag gctgctcttc ttgagctcct
       61 cctcgctgcg gagaatatca aatcgctcca ccccgtgctc cagagcgaag atgctcacga
      121 actcctcaat cgtctccata ttcctgccgt gcatcatcgc gtagagcgtg tacggccact
      181 ccgcgcacgg ggcgcgttcg tagcagtggc tcacgagcgg gctctccgcc agcagcttgc
      241 ccagccgttc catttcgtcc gggggagcct tgaagacgac catcgcgttc gccgcgtacc
      301 ccgagttctg atgccgcagc accgcgccga tccgcttcag cttccccgac gcgcggagcg
      361 cttgcaaccg tgctatcacc gtctcctcgg agacgccgag acgctcggcc agcgtccggt
      421 agggggcgac ggtcagcggg aggttctcct gaagctccgc gatcagccgc aggtcaagct
      481 cgtccatagc cctcacgctc cctccatgtc gaagaagacg cgcagtttga agcttctcag
      541 cctgggaaag ggaaagactt gcgacagagc gaaacgccgc cgcaactcct ccagaaatgc
      601 gtcccgctct tcgtcgcata cggttgagag cgtaaaccac atgttgagga agccgctcct
      661 gcggtagttg tgcgtcacgc cggggtgcgc gttcacgaag cgcgcggcat ctccgaagat
      721 atcctccggg acgtgggcgc cgacgagcat gctggagtac cccattgcgc cggagtcaaa
      781 gacgcccccc aggcgacgga taatgccttc acgcatcagc gcaccggttc gttcgacgat
      841 ctcctctctc gaaacgccga gcttcgcggc aaaaacaccg taggggtccc tctccagagg
      901 aagcccctcc tgcagcagat tgatcagctt ccggtcggcg ttgtccatgt tccgctcctg
      961 aagcaccagg gctcctccgc catatagttt ccagtgtagg agaacgcgcg cgctctgcac
     1021 ccgccgcaga tcgcaacata ctcgcatgcg ccgcagcgcc cctcgtagcg cgagaagtcg
     1081 cgcaactccg caaagacctc gctctcgcgc caaatatcgg cgaaggccgt tcttctgacg
     1141 tttcctgcct ccaccggcag gtatgggcag acgtggacct cgcctttcgg cagaatacag
     1201 cagtatgaga ctccggcgag acagccgcgc gtaaaacgct gcgcgattcc catctccttc
     1261 gccatcggca tgaactgcgg cgcgcaggtg ggcttcagtt caagcgtcgt cgtcgcctgt
     1321 cgttccagga tcttccgtat catcccgaaa tacgcgccgg atttcagttc ctcctccggc
     1381 atggaacgcg cgcggcctgt gggaacaagg aagaacgggt gaatggcgtg cacgcccgtc
     1441 gattcgtaat aagcaaccat cttgtcgaat tcttccaggt tcttttccgt gagagtcaag
     1501 ttgatctgca ccctcagccc tgcggcaagg gcgtttcgaa ttcctgcctg cgtcaggtca
     1561 taagccccgt gagagcccct gaacgcgtcg tggtactccc tgtccaggct atccacactg
     1621 atcgcgatcc ccctgagacc ggccgcggca agctcgcgcg cgaccgattc cgtcagcagc
     1681 gtcccgttcg tccccatcgc cgggacgagt ccgcggtcgc gggctgcggc gatcagttcg
     1741 agaatatcct cccgaagcag cggttcgccc ccgctgaaga taaggagacg gaactccgcc
     1801 tccgcgatag aggcgatcag ttcgattccc tccgcggtgg acagctctcc ctggatatcc
     1861 tctcccgggc cggactctct gtagcagtgc ctgcagaaga ggttgcagcg gcgcgtcgtg
     1921 ttccaggaga tcagcacgtc gcccctccgg acgcgatctc gtcgccagtg aggtaacagg
     1981 acggatcggg accccaaaaa tccccggcgg cgagcgccct ggcccggaga ttcccgttgc
     2041 actgttccag ccacccgcag gtgcggcacc ggccgcggag atgccgcttc cggtcgcgca
     2101 gacgatgcag aaatgaatcc gtctctcccc gccagagctc ggggaagtcc ctctcgcgaa
     2161 cattgccgag gatgatctcc tgcgtgaact ggtctatgtg gacatccccc tcccagtcga
     2221 ccgcgccgat cgcgacgccg gagcggttgc ctccgcttgc tgtaagcagt tccagcatct
     2281 ccgcggcgcg gctccctccc cgctctttct cccgaaggta gagatagatt ccgtcgcagt
     2341 ggttgtccac ggtgagaatt tcggtctcca cgccccgagc ggcgaagtcg agcgtctttt
     2401 cggcgaggaa atccagagcg gctcgcttct cctccggcga aggctcctca tcggtcagtt
     2461 cggcgccccg ccccgaaggg acgaggtgat acaggcagac tcgggctatt ccttcgtctt
     2521 cgacgagacg aaagatctcg ggcagctcct tcagcgtact ccgggtcatg gagagacgca
     2581 atcccgcctt ctgccccgct ttccggcagt tcctgaatcc ggagatcgtc ctgtcgtagg
     2641 ctccgtcgac gccgcggaat gcgtcgttta cggagcgcga tccgtcgaga ctgataccgg
     2701 catagctcac gccgagttcc attatgcgcg ccgcagtccg ttcgtcgatg agcgtcccgt
     2761 tggtcgagag cgcggtgcgc aaccccagcg tgcgcgcgta ctccaaaagc tcgaagaggt
     2821 ccctccgcaa caggggctct ccgcccgaga gcagcaaggc ggggacgttg aaggcggcga
     2881 gggtatccag aaaatccttc gcctcgcccg tcgaaagctc ggcgtcgcag gcatacggcg
     2941 tcgccgaggc gtagcagtgg cgacagagaa gattacacgc gcgggtgcag ttccagacga
     3001 caactggtcc caaaccgtcg gcagcgcccg cgctgttcgt tccgcggcgg taccgcagtc
     3061 gatcgccgga atactcgcgt ccgagcagca attttgtcac actgatcatc gaaaccctcc
     3121 ttgaggaaag tattcggcga gctgtatcag ccaacggcgc tgtacggata aattacccga
     3181 tgagcagcca ttcgatcatc gggacaaaca tgttccatca cgacgccaca agcattgtat
     3241 ttgtcccttt cccggatgta aagcgcgaag cgccgttcat accactgaaa caggggcaaa
     3301 aatcacacgc tcttttgata taattgccgc aaattccgat ggcgaacctc ggccttaaag
     3361 ccgttcatcg ccgtgaaagg tggtaccgaa tgcgctacac aaccgctctg cccggcttga
     3421 tcatccggga tgccgtggaa caggatgttc cgctcatcct ctcgttcatc cgggaacttg
     3481 ccgtttatga aaaaatgctc gacgacgtcg tcgccacaga ggacggactg aaagagcacc
     3541 tgttcggcga acgccgcgtc gcagaagttc tcatagccga gtgggagggc gctcccgtcg
     3601 ccttcgcgct cttcttccac aacttctcaa ccttccttgg acgtccggga atctacctgg
     3661 aggacctcta tgtgaagccg gagatgcgcg ggaaggggat cgggaaggcg atgctctccc
     3721 acatcgcgaa catcgcaaag aaacgcgggt gcggccgttt cgagtggtcg gtgctcgact
     3781 ggaacgaacc ggcgatcaca ttctaccgct ccatcggagc agtggcgatg gacgagtggg
     3841 agcacttccg cctgagcggg gaggctctgg aaacggtcgc cgaaatgtgt gcaccggaga
     3901 acgtgtatcc caaacagtga agaaaacgct cactcgatac gacaaagagc ggggtcccat
     3961 ccgagggaac cccgctcttt gttgcatcct acgcttacac cttccgaagc accttccccg
     4021 ccgcggcgcc ggtgtactcg ccgttcttca cggcgagacg ccccgccaca aagacgtggg
     4081 agatgccctt cggctcgccg aaggggtcct cgaaggtcgc tccgtcgacg acggtctccg
     4141 ggtcgaagag gacgagatcg gcgtccattc cttctctgat gagtcctttt tttgacagcc
     4201 cgagccgtgc tgccgggaag ccgctcatct tccaaacggc ctcttcgaga cgaagggtct
     4261 tttcctcccg gacgtatttg ccgagaacgc ggggaaacgt cccgttcgta cgtgggtggc
     4321 acttcgctcc gggcgccgga ggaatgctgt cggaagccac catgacgaga gggtgcttca
     4381 tgacgaactg cacgtcttcc tccgcaagct gaaagaagca tgccgtgtcg gaacccctgt
     4441 tttcaataat gatatcgaat gccgcgtcga gcggatcctt ccccgtcatc gcaccgatct
     4501 ccgtcaggcg ttttctctcg aagtccggcg ttttcgggca gtagagcatc atcacaccct
     4561 ccggaccgcc gctgttgcgc agcatgttct cccacccttc ggcctccggg atctcccgtt
     4621 tcagacgctc gcgaagcgcg gggccggaga gacgttcgat cagcttctcc acccctccct
     4681 cgtgaatcca ggggggaagg atgctccgga gcgtggtgct ccccgccgca tacggatact
     4741 gatccgccgt catgtcgacg ccgcgctccc tggcctcctc aagcagtctc atggtctcac
     4801 ggaccctgcc ccggttcctc ctgccgcagg ccttgtggtg gagcacctgc cccggaatcc
     4861 cgaggttttc cgcaacggcg atggtttcac gaacagaagc gacaacatcg tccgactcgc
     4921 tgcgcatgtg ggagagatag agccctctcc gttcaacaag gcctttggca atctcccgga
     4981 cctcctccgg agccgaatag actcccggag cgtagacgag gccgctggtc attccaaagg
     5041 ccccctcctc catcgcgcgg cgcgcatgtc cccgcatcgc agccagctcg ccgctcgacg
     5101 gcgttctgtc ctcgaaaccg aggacgttga gccgaatcgt gccgtggccg acaaacgagc
     5161 cgacgttgac gccgagctcc attccgccga gcacgtcgag atactctccg aaggagcgcc
     5221 agttccacag gggcaacgct cccgccatga taaagccggt gtaggattcc agcagcgcca
     5281 ccttctccgg cgttatgggc gcggcgctga tgccgcactg ccccaccatg acggtggtga
     5341 ctccctgctt cagcttgggg acagacgccg gttccttcaa cagaaggaag tcgttgtgcg
     5401 tgtgcatatc gataaaaccc ggggcgagca cacgctccgc agcgtcgatc tccgtagcgg
     5461 tctccgtatc cgggggcaga gttcctacaa agacaacact gccgtcacgg actcccacgt
     5521 cgccccggta ccaaggggag ttcgacccgt cgacgatacg cgcgttccgc aggaccaggt
     5581 cgaacccccg tgtttcaata cggagtgcca cgcctatttc accgtgaact tcaccgtacc
     5641 cagcggctct atggagtaga cgtactcgcc gggcttgccg gggatcatgt tcccgagcgc
     5701 cccggtgagc aggacgtcgc ccttctccat cgtccaccct tcctcgacca tcttgttcac
     5761 cagccaaaga agcgccttcc actggtcgcc gagcgcgtcg gaaccccttc cgcgggttgc
     5821 ttccacaccg tccagcgtca tcacgggaac cagctcgttc aggtcgggcg cgtccgcgag
     5881 ggggatccgc ttcccaagga tccacgcctt cgacgaaatg gcggacgcgt tgatatccat
     5941 agccttcagc agcttcatat cggtgaagga gaggtccgga agttccacgg cggccgcgac
     6001 cgactcaatc ttcgccttga gttcagcctc gtccttcagc gaggcgtcga ccggctcgcc
     6061 caggatgaag gcgatctccg cttccagcat cagaacgccg aaagaagcgc ggtcgacgac
     6121 gacgctctcc gcgctcccgt ccatcgcacc ctcggggaag agcggcgcgg cgaagggcgt
     6181 ggaggccccg aaacgcttca tcgtcgcctc ggtggtgagc ccggccttga accccgccgg
     6241 acgcttccct tccaggcgtt tcaaagaata tgccttctgt acggcgtacg agccggcctc
     6301 cgtcatatcg gcgtactccg cggagaggac ggggaaagga gtcccttcca cggcggcaag
     6361 atagactttg tccgccgcct gctccacgtc catcgcaacc gccgcgcccg cgcacacaac
     6421 cgcgatcagc gccgctaccc aaaaactccg tgccactgtt ttccgaaaca cattcaacac
     6481 ctctttcaca aaaaagtcgg atcttcagac tgcgcgcagc ctttcaatct gtggtgtatt
     6541 atactcttcc ttttttcggt tttcccggca tactcttctc aagtgaagag atgctacaat
     6601 agtaccgacg ctacgatacg ccttctcggc ggggaagttg ggggaaaaga gttgaagcga
     6661 attcttgttg tggacgacag cgctgtgagc cggaaactcg ctctcttcgt ccttaaaaat
     6721 cttgggtgcg aggcgcgagg agtaccctcg gccgccgagg ccttcgaagc gctgcgaaat
     6781 gaagtctacg acgcaatctt tatggatctg cacatgccgg aaatgaacgg ctgcgaagcg
     6841 tcgatgttca tccgccggtc aggcgccgga gaagccaacc gctctctgcc gattttcgcg
     6901 attactggaa gcgatctgcc cgaggaacga gaaagctgcg tatcggcagg tatgacagat
     6961 ttcctggcaa aaccgttctc cggagaacat gcggaagcgc tgctgagaaa acattgcgga
     7021 aagactgcac acgatggaca ataatccggg gtgtgctata attggttgaa tagatagttt
     7081 ttaaagggag gaggcaatgg atatgtccgg tatttcggct gtacctcctg taaacagcat
     7141 caagacagtc gttcccgtag ccaacagaac ccggcgtgaa ggtttgcagc gcgccgaacg
     7201 ggaagtcatg cgccaggagc tggccgtgag gaaccagggc gacgtcacct ccaccgtcta
     7261 ccactacgaa ataggtcccg acggaaggcg ctacattacc ggagctacgg taacctacaa
     7321 agaaaaagaa aacgagctct cctcaaagtc tgcgaaggac aaatcttcag cgagctctgc
     7381 aaatacagga cccgcctcct ccgcagaaaa cggccgcgtc gagctcactg acgagagccg
     7441 ggccaagttg aaggcgatcg aacgcgacgt catcgcgcac gaagccgccc acaaggcagt
     7501 cggcggacag tatgccggac cggtttccta tacgtac
//
LOCUS       DAOPKU010000078         7467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10548_length_7467_cov_4.543443, whole genome shotgun sequence.
ACCESSION   DAOPKU010000078 DAOPKU010000000
VERSION     DAOPKU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10548_length_7467_cov_4.543443"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..945)
                     /locus_tag="PK849_03680"
     CDS             complement(154..945)
                     /locus_tag="PK849_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HPE65257.1"
                     /translation="MDILRRSASLLTPEAWEELDEQAKKVLVANLSARKFVDVEGPKG
                     WTYSAHPTGRLEIDASNSGKSVQFGVNKVLPLVETRATFELDIWELDNISRGAKDPDL
                     SALEKAAKEIALFEEQAIYKGLATAGIEGLATAAKGSSVSLESEEPHGIVNGLSAAVF
                     ALREKAVEGPYAFVASPKLWKAIYSATPGYPTSKHVANLVDKVILSTQDESYVVSLRG
                     GDFELVLGQDLSLGFEERNGGKVRLFFAESFTFRVITPEAIVSLT"
     gene            complement(987..1334)
                     /locus_tag="PK849_03685"
     CDS             complement(987..1334)
                     /locus_tag="PK849_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPE65258.1"
                     /translation="MQYTEPADKLDQQTVDQAQALKSLMEEVEAVNYYNQRAALTPNA
                     ELKALLAHNRDEEIEHAAMLIEWLRRNMDGWDEELKTYLFTSAPLTQVESGGGAGAES
                     GVSSKPLGIGSLK"
     gene            1627..2271
                     /locus_tag="PK849_03690"
     CDS             1627..2271
                     /locus_tag="PK849_03690"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HPE65259.1"
                     /translation="MDEQGKKTLLSEDIRELLSLKPGENVLFGELAAAVADRGFGILL
                     VLLSLPSALPVPAPGYSTPFRIVLFILGVQMLLGRETPWIPGWAGRREIKRKTADRMI
                     SGAAKFFSFVEIFIRPRFEFVSGKGAGMICSLLLLLMSGLMILPIPLTNTLPAMVIFC
                     IGVALTERDGLAFIAALLFGAAATALYALAVWVIFYLGAQGIGEAKEIIKGWFF"
     gene            2351..4654
                     /locus_tag="PK849_03695"
     CDS             2351..4654
                     /locus_tag="PK849_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPE65260.1"
                     /translation="MSIRRKMLVLIAGFLLVTLVLTFGSYVLTGRSVRVQVEDTGRTM
                     VAEIASAMDEYIAKLLSLTGSLAVSLSYISEEGDPDYVELFGRYLEAAQTHGVQTVFM
                     GFESKEFADSTAWEPPEGYDPRTRSWYVETLKKNGVTVTSPYVDLITGELIVSVTAPV
                     RSQSGMLLGVAGLDVSLEHVRRKVLTRRLFGEGFPFLVDRHGFFLAAPFEDWTLAENI
                     ALRSVAIPAELAEIGEGILERESGAFSLTFKGERMILFHAPVGEHFSFGMFFPESAMN
                     SFVRDISSLHLFGGFAIILLALVLLLPLTHEILSSFSSLSSTIDSIAAKLSDNKDFTE
                     TAFNVHLLAAEVGESMTRTSLLEFQTFLGSMENALKTIGRQGEEIAALTEEALAIQDN
                     LTEANGELNRRQKIWRDTLEVMETMTMSGESEKNLRSVAENILKSTQAFGVLLSCRRG
                     DRFFNLVCAGYGGASLPSGDAIAIFGSVAGRAVREGDAVWVEDVARDREYVPVHPSVV
                     SEVEIPLLHRGKANGILEVAFDRRTPRNDDLLETLLPVASALGSLIEAENAHIEIKDS
                     YRYLVRKLQSVTEIYHLESADHMDRIGAYSRLAARILGKNEEEQEDIEVFSRLHDIGK
                     LRVPLSILAKPGKLTDEEMKIVRNHPLWGAELIGGAKWLDMARKICLSHHEKWDGSGY
                     PLGLSGDEIPWEGQVVALADVYDALRSRRVYKDRMAHEEAVRIILHGDGRTAPEHFSP
                     LMLDFFREHHEELDKIYGAFFLGEAEK"
     gene            4900..6081
                     /gene="carA"
                     /locus_tag="PK849_03700"
     CDS             4900..6081
                     /gene="carA"
                     /locus_tag="PK849_03700"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPE65261.1"
                     /translation="MSNCFLVLEDGEVFKGVSFGAPAPRADEFLGGGAAGGGEVVFNT
                     AMSGYHEMLTDPSYAGQIVVLTSPHAGNYGCDDAWSERGPDDPGMAEIKLRGLVVRSC
                     FSGPVPRGRISLDAFLKNHGIPGLADVDTRSLTIRLREKGSCNSFIVAPSAGDALSVE
                     DIRSVASSFRRLPSMEGANLLASVGTRTALPASFGNHPRFTVVDCGVKGNILRELGAR
                     GAAVTVLPWTSSSEDVLSSTPDGVLFSNGPGDPAALSSVVTTIRNLIGVVPVFGICLG
                     HQMIALALGGRTRKMKFGHHGCNHPVRDERTGKVQVTSQNHGFEVDEQSLPPDVTVWF
                     RNANDGSVEGLMHGRLPVMSCQFHPEAAPGPRDSSWIFDEFVGIASRRKEGSSCLLET
                     I"
     gene            6060..>7467
                     /gene="carB"
                     /locus_tag="PK849_03705"
     CDS             6060..>7467
                     /gene="carB"
                     /locus_tag="PK849_03705"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPE65262.1"
                     /translation="MPARNDLKKILVLGSGAIVIGQACEFDYSGVQAVKALKEEGYKV
                     VLANPNPATVMTTPGIADAIYLEPLKLPWVEEIIRCERPDALLPTMGGQTALNLALEL
                     ADAGILERYGVEIIGASVRSIRLAEDRGAFKDAVRSIGLDTPRSAVVRSAAEAFAFAE
                     ETPLPLIIRPSFTLGGLGGSIASTRQQLAIQVERALAESPVASALLEESLIGWKEFEM
                     EVMRDRRDNAVVVCSIENIDPMGVHTGDSVTVAPIQTLSDRAYQEMRTASLNILRVVG
                     VDCGGSNVQFAVHPESGRMVVIEMNPRVSRSSALASKATGFPIARAAAKLAVGYTLDE
                     IPNEITGKTTSCFEPALDYVAVKVPRFETEKFPAEYECLGTQMKSVGEALALGRTFME
                     ALNKAIRAAEAGYDGLQELEIGYDGLDRMLAPLHPRKLFAAYTVLCREGERALPLLAR
                     KTGIDPWFLYQMAEQADFE"
BASE COUNT         1508 a         1986 c         2263 g         1710 t
ORIGIN      
        1 gaagagggtc tttcagtgcg gattatagag gtttttgggg gaaatacaag aggggaattc
       61 cgttgcggga gagctttttc gcaggctgtg cgacgcaaaa aaacaggggc tcgggagagc
      121 cccttttctg gagttcgcaa agcaagtttt ctgttaggta agcgaaacga tggcttcggg
      181 ggttatgact ctgaatgtga atgactcggc gaagaaaagt cgcacctttc cgccgttccg
      241 ctcctcgaag ccgagcgaaa gatcttgtcc gagaacgagt tcaaagtcgc cgccgcgcag
      301 cgagacaacg tacgactcgt cctgggtgga gaggatgacc ttgtcgacga gattggcgac
      361 gtgttttgat gtcggatacc ccggtgttgc gctgtatatg gctttccaga gtttcggaga
      421 tgcaacgaat gcgtatggac cttcgactgc cttctcccgg agagcgaaca ctgccgctga
      481 aagaccgttg acgattccat gcggctcctc gctctcaaga gaaacggagc ttcctttcgc
      541 agcggtcgca agaccctcaa tacctgccgt agccaagccc ttgtaaattg cctgttcctc
      601 gaaaagggcg atctccttcg cggccttttc aagcgcggac aggtcggggt ctttggctcc
      661 tctgctgatg ttgtcgagct cccaaatatc gagttcaaac gttgcccggg tctccaccag
      721 aggaagcacc ttgttgacgc cgaactggac gctttttccg gagtttgagg cgtcgatctc
      781 cagccttcct gtcgggtgtg cgctgtatgt ccaccccttg gggccttcga cgtcgacgaa
      841 ctttcgagcc gaaaggttcg caacgagtac ttttttcgcc tgttcatcaa gctcctccca
      901 agcctccggg gtcaggagcg atgcggatct tctcagaata tccatgagtg tgtacctcct
      961 ttacatgttc gtctgctctg caggaactac ttgaggctcc cgatgccgag gggcttggaa
     1021 gaaacgccgg attcggcgcc tgctcctccc ccggattcaa cctgagtgag aggggccgaa
     1081 gtgaagagat atgtcttcaa ttcttcatcc catccgtcca tatttctccg aagccattcg
     1141 atgagcattg cggcgtgttc tatctcttcg tcccggttat gagcgaggag cgctttgagc
     1201 tccgcgttcg gcgtgagtgc ggctcgctgg ttgtagtagt ttaccgcttc tacttcctcc
     1261 atgagacttt tgagcgcctg cgcttgatca actgtctgct gatcaagctt atctgcgggt
     1321 tcagtgtatt gcatggtatc tctcctttct ggaaatcact tttagatgat gccgaccgtg
     1381 cggactcggc atttattcca acattatacc gtctgcagag gaaaatcact cgtgtaaaaa
     1441 agagacggtt ctaaataaat ttattacaag gtagttttta ttttatgtga gtatatacca
     1501 acttctaact ttcgtcaagt ttggtcaaat ggaagaatcg cttcactgta tgatatcatg
     1561 cggtgcgaag tgtgggggat gtccgctgaa aagcgtatca tgacgagggg aaggtttgtg
     1621 tacattatgg atgaacaggg gaagaaaacg ctgttatcgg aagatattcg agagctgctc
     1681 tcgttgaaac ctggagagaa cgtcttgttc ggagaacttg cagcagctgt ggctgatcgt
     1741 ggattcggca tccttctggt gctcctttcg cttccgagcg cgcttcccgt gccggcgccg
     1801 ggatacagca cgccgtttcg aatagtgctc tttatcctgg gtgtgcagat gctgctgggc
     1861 agagagacgc cgtggattcc gggatgggcg ggacgaaggg agatcaagcg gaagacggcg
     1921 gacaggatga tctcgggtgc ggcaaagttt ttttccttcg tggagatttt catacgcccc
     1981 cgtttcgagt tcgtctcggg aaagggcgca ggaatgatct gttctttgct gctgctgctt
     2041 atgagcggac tcatgattct tccgattcca ctgacgaaca cgctccccgc catggtgatc
     2101 ttctgtatcg gcgtggcgct cacagagcgc gacgggctcg cgttcatcgc ggcgctgctt
     2161 ttcggcgcgg ctgctacggc gctgtatgct ctggcagtct gggtaatttt ttacctcggg
     2221 gcccagggaa taggcgaggc gaaggagata atcaagggct ggtttttctg atcccgaact
     2281 tttctgtaca cgtcatgatt cgccaatgat ttttcagggt ttccccggaa acgagagggg
     2341 tgatactgct gtgtctattc gacggaagat gctggtgttg atcgccgggt ttcttcttgt
     2401 aactttggta ttgactttcg gcagctacgt cctcaccgga aggagcgtgc gcgtccaggt
     2461 cgaagatacc gggcgaacta tggttgcgga gatcgcctcc gctatggatg aatatatagc
     2521 gaaacttctc tcccttactg gctcgctggc tgtttctctc tcatacatca gcgaggaggg
     2581 cgatcctgac tatgtagaac ttttcggccg ctatctggag gctgcgcaga cgcacggagt
     2641 gcaaacggtc ttcatgggct ttgaatcgaa ggagttcgcc gattctaccg catgggagcc
     2701 gccggaaggg tacgaccccc ggacgcgctc gtggtatgtg gagacgctga agaaaaacgg
     2761 tgtgacggtc acctctccat atgtcgacct gataaccgga gagctcatcg tgagcgtcac
     2821 ggcgcctgta cgaagccagt cggggatgtt gctcggtgtg gcggggctgg atgtgagctt
     2881 ggagcacgtg cgaagaaaag tgctcaccag aaggctgttc ggagaaggtt ttccattcct
     2941 tgtcgacaga cacggttttt tccttgcagc tcccttcgaa gactggacgc ttgcggaaaa
     3001 catcgcgctt cgctccgtgg cgatccctgc ggagttggct gaaatcggcg aaggcatcct
     3061 ggagagggaa tccggggctt tcagcctaac gttcaaggga gagaggatga ttctctttca
     3121 cgccccggtc ggcgagcatt tttcgttcgg aatgttcttc cccgagagcg cgatgaacag
     3181 ctttgtcagg gatatatctt cccttcatct cttcggaggg ttcgcaatca ttctgctcgc
     3241 tcttgtcttg ctgcttcctc taacgcatga gatattgagt tccttttctt cgctctcctc
     3301 gacgatcgac agtatcgccg ccaaactctc cgacaacaag gacttcaccg aaaccgcgtt
     3361 caacgtccac ttgctggctg ctgaagtggg ggaatctatg acgcgcacgt cgcttcttga
     3421 gtttcaaaca tttttgggga gtatggagaa tgctctgaag acgatcggac ggcagggcga
     3481 agagattgcc gcgcttaccg aggaggctct tgcgattcag gacaacctca cagaagccaa
     3541 cggagagctg aatcggagac agaaaatatg gagagatact ctcgaggtca tggaaacgat
     3601 gaccatgtcg ggcgagtcgg agaaaaacct ccgaagcgtc gccgagaaca tactgaagag
     3661 cacgcaggct ttcggggtgc ttctttcctg ccgaagaggc gatcgttttt tcaacctcgt
     3721 ctgtgcggga tacggcggcg cttcgctccc gtccggcgat gcaatagcaa tcttcggaag
     3781 cgtcgcgggc agagctgtgc gtgaggggga tgccgtttgg gtcgaagatg tggctcgtga
     3841 ccgcgagtat gttcccgtgc atccgagcgt ggtcagcgaa gtcgaaatcc ccttgctcca
     3901 ccgcgggaag gcgaacggga ttctcgaagt ggccttcgac aggagaacac ccagaaacga
     3961 cgatctgctc gaaacgcttc tccccgtggc gtccgcgctt ggaagcttga tagaagccga
     4021 gaatgcgcac atcgagataa aagactccta tcgctaccta gtccgtaagc ttcagtcggt
     4081 aacggagatc tatcacctcg aatcggcgga ccacatggac agaatagggg cgtactcccg
     4141 tctcgcggcg cgcattttgg gcaagaacga agaagagcaa gaggatattg aggtgttttc
     4201 caggctgcac gacataggaa aacttcgcgt gccgctctcg attctggcaa aacccggaaa
     4261 actcaccgac gaagagatga agatagttcg caatcatccg ctctgggggg cggagctgat
     4321 cggcggcgcg aagtggcttg atatggccag gaagatctgt ctctcccatc atgaaaagtg
     4381 ggacggcagc ggctatcctc tcggcctttc gggagacgaa ataccttggg aggggcaggt
     4441 cgtggcgctt gcggatgtgt acgatgcgct gcgttccagg cgggtgtaca aagaccgaat
     4501 ggcgcacgag gaggcggtac ggattatcct gcacggcgac ggacgaaccg cgccggagca
     4561 cttcagcccc ctgatgctcg acttcttcag agaacatcat gaggaattgg acaaaatcta
     4621 cggagcattc tttcttgggg aggctgagaa gtagacgcct cattgctgaa ggagtgatct
     4681 caacgcattc tctgaatgca cggaacggga aaaatacgtg tgggagagtc cctgctcttt
     4741 tgtgcaggaa ctttcccaca cttttattta aggaatgcgt tatttataag ctctatgggt
     4801 tgacaaaacc ggcccatttt tgtatcctcc cttggaacat aagtttatta ttttgctaaa
     4861 gatgaaaatc tatttagtaa aaagagggag aggcgaatca tgtcgaactg ttttcttgtg
     4921 cttgaggacg gggaggtctt caaaggcgtt tctttcggag cgccggcgcc ccgagcggac
     4981 gaattcctcg gtggcggagc tgccggggga ggggaggtcg tcttcaatac cgcgatgtcg
     5041 gggtatcacg agatgttgac ggacccgtcc tacgcagggc agatcgtcgt gctcacctcc
     5101 ccccacgcgg ggaactacgg ctgcgacgac gcctggtcgg agagggggcc ggacgatccg
     5161 ggaatggcgg agataaagct gcgcgggctt gtggtgcgtt cgtgcttctc cggtcccgtt
     5221 ccccgcggac gcatctccct cgacgcgttc ctcaaaaatc atggaattcc cggtcttgcc
     5281 gatgtggaca cgcgctcatt gacgatccgc ctgcgtgaga aggggagctg caacagcttc
     5341 attgtcgctc cgtcagcggg ggatgcgctg agcgtggagg acattcgttc cgttgcgtcg
     5401 tcgttccgtc ggcttccctc aatggaagga gcgaatcttc tcgcctcggt gggcacgcga
     5461 acggcgcttc ccgcttcttt cggtaatcac cctcgtttca ccgttgtcga ctgcggggtg
     5521 aaggggaata tccttcgcga actcggggcg aggggcgccg cagtcacggt gcttccatgg
     5581 acctcgtcgt cggaggatgt cctgtcgagc acccccgacg gcgtgctctt ctcgaacggc
     5641 cccggcgacc ctgccgcgct ctcgtcggtg gtgacaacca tccgcaatct catcggcgtc
     5701 gttccggttt tcggtatctg cctcgggcat cagatgatcg ccctcgcgct cggaggccgg
     5761 acgcggaaga tgaagttcgg ccatcacggc tgcaatcatc ccgtcaggga cgaacggacc
     5821 ggcaaggtgc aggttaccag tcagaatcac ggcttcgagg tggatgaaca gtctcttccg
     5881 ccggatgtga ccgtatggtt ccggaacgcc aacgacggct ccgtcgaggg gctgatgcac
     5941 gggcgccttc cggtgatgtc gtgccagttc caccccgagg ccgctccggg gccccgcgat
     6001 tcctcgtgga ttttcgacga gttcgtcggg atcgcctcca ggagaaagga gggatcctcg
     6061 tgcctgctcg aaacgatctg aagaagatcc tcgttctcgg gtcgggcgcc atcgtcatcg
     6121 ggcaggcgtg cgagttcgac tactcggggg tgcaggcggt caaagcgctg aaggaagagg
     6181 ggtacaaggt ggtgctcgcc aaccccaatc ctgccacggt gatgaccact cccggaatcg
     6241 ccgacgccat ctacctcgaa ccgttgaagc ttccgtgggt ggaggagatc atccgctgcg
     6301 agcgccccga cgcgttgctg ccgaccatgg gagggcagac tgcgctgaat cttgcccttg
     6361 aactcgccga cgcaggcatc ttggagcggt acggcgtcga gatcatcggc gcttcggttc
     6421 gctccatccg tcttgccgaa gacagggggg cgttcaaaga cgccgttcgc tcgatcggac
     6481 tggacacccc gcgttccgct gttgttcgca gcgctgcgga agcgttcgcc ttcgccgaag
     6541 agacgccgct ccctctgatc attcggccgt cgtttacgct gggcggcctg ggaggttcca
     6601 ttgcctccac acgacaacag ctggcgattc aggtcgagcg cgctcttgcg gagagcccgg
     6661 ttgcttccgc gcttcttgag gagtcgctca taggatggaa ggagttcgag atggaggtta
     6721 tgcgcgaccg cagggacaac gccgtggtcg tctgttccat tgaaaacatc gatcctatgg
     6781 gagtgcacac gggggacagc gtgaccgtcg ccccgatcca gacgctcagc gacagagcgt
     6841 accaggagat gcgcacggcg tcgctgaaca ttctcagagt cgtcggcgtg gactgcggcg
     6901 gttccaacgt gcagttcgcc gtgcatcctg agtcggggcg catggttgtc atcgagatga
     6961 acccccgcgt gagccgctct tccgcgctgg catcgaaagc gacgggcttc ccgatcgccc
     7021 gcgcggcggc caaactcgca gtcggctata ccctcgacga gataccgaat gagattacgg
     7081 gcaagacgac gagctgcttc gaacctgcgc tcgactacgt cgcggtgaag gtcccccgct
     7141 tcgaaacgga aaagtttcct gcggagtacg aatgcctcgg gacgcagatg aagtccgtcg
     7201 gcgaggcgct cgccctcggc cggacgttca tggaggcgct gaacaaggcc atacgggccg
     7261 cagaggcggg atacgacggc ctgcaggagc tggagatcgg gtatgacggt ttggaccgca
     7321 tgctcgctcc tctgcatccg agaaaactct tcgccgccta tactgtactc tgtcgcgagg
     7381 gagaacgagc gcttccgttg ttggcgcgaa agacaggcat tgatccgtgg ttcctgtacc
     7441 agatggctga acaggccgat ttcgagg
//
LOCUS       DAOPKU010000079         7459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10557_length_7459_cov_4.518098, whole genome shotgun sequence.
ACCESSION   DAOPKU010000079 DAOPKU010000000
VERSION     DAOPKU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10557_length_7459_cov_4.518098"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1744
                     /gene="ligA"
                     /locus_tag="PK849_03710"
     CDS             <1..1744
                     /gene="ligA"
                     /locus_tag="PK849_03710"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPE65263.1"
                     /translation="LTRTAPFAGEHGYLCELKIDGLAVSLVYENGVFAQGTTRGDGRT
                     GEDVTANIRTIRNLPLRISGAFPPKIEVRGEVLLQRQHFADLNVEREERGEPLFANPR
                     NAAAGSLRQLDPAVVAERRLSIFLYTVVDPVLFDLKTQEEALRKLAVLGLPVQPAWRR
                     CAAPEDVLAFVEEQREGRFSLPYATDGVVVKLNSIAAWDELGATSHAPRWAIAFKYPP
                     EEKSTRLEDIIVSVGRTGALTPVAVLSPVALSGSVVRRASLHNEDELRRKDVRIGDMV
                     RVRKAGEIIPEILGPVLDARTGEEREFIMPKHCPACGTPAVRLEGEVALRCPNRSSCP
                     AQLKEGLRHFASRSGMDIRGLGDKIVDQLVEKGLLRSLADLYDLNEQTLASLDRMGEK
                     SARNLINALEQSKERPFSRLLSALGIRFTGSRSAEILAEVFRDLPTLRDASEETLAAV
                     DGVGPVMASAIRSFFEQPENQRLLERLEAAGVRATLPRLEDERENEHIGSRPFAGMRL
                     VFTGEMESMTRSEAEEMAKRRGAVCSSSVSKKTSFVVAGKDAGSKLSKALSLGVKVVD
                     EEEFLEMTRLTDEA"
     gene            1961..2317
                     /gene="gatC"
                     /locus_tag="PK849_03715"
     CDS             1961..2317
                     /gene="gatC"
                     /locus_tag="PK849_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPE65264.1"
                     /translation="MTPRDSSACALDTKAVRHVADLARLRVEDEEMDGLVRHFGAILE
                     HFNVLSAAGKDGRLDLDAVDPFLFSDTEIPPAREDVPIVSSVRGDVLGSAPRRKDSFF
                     VVPRILAQHDGEEFSS"
     gene            2314..3882
                     /gene="gatA"
                     /locus_tag="PK849_03720"
     CDS             2314..3882
                     /gene="gatA"
                     /locus_tag="PK849_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPE65265.1"
                     /translation="MSLCSLSAVEIARGIRNRDFSALEILRSFYQRAETLEPSLHALL
                     HCAPEIAEKHAADVDAAVARGEDPGPLAGVPVVLKDNMCVRGMPATCASRILQGWIPP
                     YDATVTELLETSGAVVAAKGNMDEFAMGSSTEFSAFGPVSNPWDTERVPGGSSGGSAV
                     SVAAGYTPLALGSDTGGSIRQPASFCGIYGLKPTYGLVSRWGLVAFASSLDQIGPFAR
                     TVEDLALSLSVIARPDPRDATSSRRERPDYFEALAPWKTPGRENAPLSGKRIAVVKEL
                     QNRKDAVSAEVEDVLQKTLALCREEGAEIVEISLPVSLEFGLPCYYILAPAEASSNLA
                     RFDGVRYGLSSDEKSLVNLYFKTRGSGFGSEVKRRILTGTYVLSSGYYDAYYLTAQKV
                     RQLMKREFAAAFAGCDLIALPTAPSPAFRKGELVDDPIQMYLADVFTLPVNLAGLPAL
                     SLNGGFTSGEKPLPVGVQFIAPKWGEPELLSTAAVLEKKLGKAKTAPCDWAETSAERA
                     ANAKTAGSERGGVR"
     gene            3879..5372
                     /gene="gatB"
                     /locus_tag="PK849_03725"
     CDS             3879..5372
                     /gene="gatB"
                     /locus_tag="PK849_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPE65266.1"
                     /translation="MKYTYQPVIGLEIHVQLATATKIFCSCSTDYIGAEPNTNVCPVC
                     LGLPGALPVLNGKAVELGIKAAMALSCTVKDKTVFHRKNYFYPDLPKAYQISQYDLPL
                     AEGGELFISGDGDTLKRIRITRLHLEEDAGKLVHSAADGRLEGAEFSFVDYNRGGVPL
                     AEIVSEPDLRSPGEAREYVTSLRRLVRYLGVSDGDMERGSMRVDANISLQKTDAATGE
                     ILYWGERAEIKNMNSLRAIERALEYEIRRQIDVMEAGGVLERETRHWNDGEGTTTSMR
                     SKEAAHDYRYFPDPDLPPIEASPALLDQVSASLPEMPWAREARFRNELGLPEADAALL
                     AETPQLADYFESCVLTGASPSRISNWVRTEVLRVLNERNISIDSFSMQPKTLGSLVLL
                     VDEGKLSTTAARETFNAMIEKGLPLEKALEASGGAGGKVSGDALADTIRAVLASAPDV
                     VEEILGGKDDKGKKKKFLTGLVMKETRGQADAAAVWELIEKLLNEKP"
     gene            5526..>7459
                     /gene="fusA"
                     /locus_tag="PK849_03730"
     CDS             5526..>7459
                     /gene="fusA"
                     /locus_tag="PK849_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPE65267.1"
                     /translation="MGTRQPENTRSFAIAAHGGAGKTSLVEAMLFDSGSTSRLGKVED
                     GNTVSDFSQEEQKRQISINTSLITMERNGKTLFVLDTPGFADFVGEMRSAVRVADTLV
                     IAVSGTSGIEVQTDKAWQTGEEFGLPVAFYISKLDRENADFGKVLSDIKAVYSEKAVP
                     VFLPIGSESSFKGVVDILRSKAYIYEINGSGKFSEGDVPADMAADVASARETLVETVV
                     EADDEMMMRYLDGESISDDELLPVLRKAIASRLLLPVFPGSSTQNVGVHQILDFIVDF
                     FPSPLEARTRPAKKGDKDIEVAPDPSATFSAICFKVMVDPYVGKLSFIRVNSGTLTSD
                     NNIFNVNKGEDERISSFKFMTGKEGKDVKEIVVGDILTLPKLQSVQVGDTLGMKGSTV
                     IYPPIDFPKPVYSVAITAKSRADEDKLGNAINKTLEEDPSLSFEKNAETNDSVLSGMG
                     DMHIDIVLSKMKERYGVDLETKTPQVPYRETIRKTAEAQGKHKKQTGGHGQYGDAHIR
                     YTPLERGAGFVFEDNIVGGAIPKGFIPAVEKGLRESLVKGPLAGFPVVDFKATLFFGS
                     YHDVDSSEMAFKLAARLSFKKGILDAGPILLEPIMNVEVTVPEDYLGDVMGDFNSRRG
                     RILGIDSKGHQQIVKAQAPLA"
BASE COUNT         1609 a         2098 c         2207 g         1545 t
ORIGIN      
        1 tctcacccgt acagcaccct tcgccggtga acatggctat ctttgcgagc tgaaaatcga
       61 cggccttgcc gtttctcttg tgtatgagaa tggcgtcttt gcgcagggaa cgacacgcgg
      121 ggacgggagg acgggcgaag atgtcaccgc gaatattagg acgatacgca atctcccgct
      181 gcggatctcg ggcgcatttc cgccgaagat tgaggtccgc ggcgaggtgt tgcttcagcg
      241 gcagcacttc gccgatctga acgtggagcg ggaggagagg ggggagcccc tcttcgccaa
      301 cccgaggaat gcagccgccg gaagtctgcg gcaacttgat cccgctgtcg tggcggaacg
      361 ccgcctctca attttcctct acactgtcgt cgaccccgtt ctcttcgatc tcaagacgca
      421 ggaagaggcc cttcgcaagc ttgcagtcct tggacttccg gtgcagcccg cttggaggcg
      481 ttgcgccgcg ccggaagatg tgctcgcatt cgtcgaagag cagcgtgagg ggcgcttttc
      541 gctcccctac gcaacggacg gcgtcgtggt caagctgaac tccatcgccg catgggacga
      601 actcggagca acgtcgcatg cgccccgctg ggcgatcgcc ttcaaatacc cgccggaaga
      661 aaagagtact cgcttggagg atatcatcgt ctccgtcgga cgcaccggcg cccttactcc
      721 ggttgcggtg ctctcacctg ttgctctttc aggctcggtc gtgcggagag caagtctcca
      781 caacgaagac gaactccgcc gcaaggatgt acgcataggc gatatggtac gtgtccgcaa
      841 ggccggcgag atcatcccgg aaatactcgg tccggtgctc gacgcgcgga cgggggagga
      901 acgcgagttc atcatgccca agcattgccc cgcatgcgga acgccggcgg tacgacttga
      961 aggagaggtt gcgcttcgtt gtccgaaccg ttcctcctgt ccggcccagc tcaaggaagg
     1021 gctcaggcac ttcgcctcgc gcagcgggat ggatatccgg ggactgggag acaagatcgt
     1081 cgaccagctc gtcgagaagg gcctgcttcg ttcgcttgcc gatctttacg atctgaacga
     1141 gcagacgctc gcctcgcttg accggatggg ggagaagtcc gcacggaacc ttatcaacgc
     1201 gctggaacag tcaaaagagc gtccgttttc ccgccttctc tccgccctcg gcattcgttt
     1261 taccggttct cggagcgcgg agattcttgc ggaggtattc cgtgacctgc cgacgcttcg
     1321 agacgcatcc gaggagactc ttgccgcagt cgacggcgtt gggccggtaa tggcgtcggc
     1381 cattcgatcg ttcttcgagc agcctgagaa tcagaggctt cttgagcgat tagaggcggc
     1441 cggagtccgg gcgacgcttc cccgacttga agacgagcgg gaaaacgaac atattggctc
     1501 ccggccgttc gcgggaatgc gcctggtttt taccggcgag atggagtcca tgacgcgcag
     1561 cgaggcggaa gaaatggcaa aacgtcgagg cgccgtttgt tcttcgagcg tcagcaagaa
     1621 gacgtctttc gtcgtggcgg gaaaggatgc ggggagcaag ctttcgaagg cgctctctct
     1681 tggcgtaaaa gtagtggatg aagaagaatt tcttgagatg acccgcctga cggacgaagc
     1741 gtaaccttca ttcgccgcgg gctgcatttc aagcagatcg aaaggaaatc gggaagaccg
     1801 gtggaatact atgcaaagcg ttcctgaacg ttctcttcga aaaacaagga gaacggaagc
     1861 ttttctctcc gtttttttcg gcgtgagaga ttcctgaatc cgtttgggaa agcgatgcgc
     1921 tctatcgttc gccgtctgct tatcaggagg tttgaatgca atgacaccca gagactctag
     1981 cgcgtgcgcg ctggacacaa aggcggtgcg gcatgtagcc gatctcgcca gactccgcgt
     2041 ggaggacgaa gaaatggatg ggcttgttcg gcacttcgga gccatccttg aacatttcaa
     2101 cgtgctctcc gctgccggga aagatggacg tctcgatctc gacgccgtcg acccgtttct
     2161 tttctcggat acggagattc ctcctgcgcg ggaagatgtc ccaatcgttt cgagtgtgcg
     2221 cggggatgtt cttggctcgg cgcctcgaag aaaggactct ttttttgtcg tgccgagaat
     2281 tctcgcgcag cacgacggag aggagttctc atcatgagtc tctgttcgct ctccgcagtc
     2341 gagatcgcca ggggaatacg gaatcgcgac ttttccgccc tggagattct caggtccttt
     2401 tatcagcgtg cagaaaccct cgaaccctct ttgcatgcac ttcttcattg tgccccggag
     2461 attgccgaaa agcatgccgc cgatgtcgac gctgccgtcg cgcgagggga agaccccggt
     2521 cctcttgccg gagtgcccgt tgtgctcaag gacaacatgt gcgtccgcgg aatgcccgcg
     2581 acttgcgcaa gcaggatttt gcaggggtgg attcctccgt acgacgctac ggtgaccgag
     2641 ctgcttgaga catccggagc tgttgttgcc gcaaagggca atatggatga attcgccatg
     2701 gggagttcaa cggagttctc ggccttcggg ccggtctcga acccttggga tacggagcgc
     2761 gttccggggg ggagctccgg cggcagcgcc gtctccgtcg ccgccggata caccccgctt
     2821 gccctcggga gcgataccgg cggttcgatc cgccaaccgg cctccttctg cggtatttac
     2881 ggcttgaaac cgacgtacgg gctcgtaagc cgatggggcc ttgtggcgtt tgcctcttcg
     2941 ctcgatcaga taggtccatt cgcccggacc gtagaagatc tcgccctttc tctctccgtg
     3001 attgcgcgcc ccgacccccg cgacgcgacc tccagcagaa gggagcgtcc cgactatttt
     3061 gaggctctgg cgccttggaa aactccggga cgggaaaacg ctcctctctc cgggaagaga
     3121 atcgccgtag tgaaggaact gcaaaacagg aaagacgccg tttccgccga ggtggaggac
     3181 gttctccaaa aaacgcttgc actctgccgg gaggaggggg ccgaaattgt tgaaatatcc
     3241 cttcccgtct cccttgaatt cggcctgccg tgctactaca tcctcgctcc tgccgaagcg
     3301 agttccaacc ttgcccggtt cgacggcgtc cgttacggcc tctcttcgga cgaaaaatcc
     3361 ttggtgaacc tctactttaa aactaggggc tccggtttcg gcagcgaagt gaaacggagg
     3421 attctcacgg gaacgtatgt tctcagctcg ggatactacg acgcctacta cctgaccgct
     3481 cagaaagtca ggcaacttat gaaaagggaa ttcgcggcgg cattcgccgg gtgtgatctc
     3541 atcgcccttc ccacggctcc ttctccggca tttcgaaagg gagagctagt ggacgacccc
     3601 atccagatgt atttagcgga tgtctttact cttcccgtga acctcgccgg actcccggcg
     3661 ctctcgttga acggggggtt cacatccggt gaaaagccgc tccctgtagg ggttcagttc
     3721 atcgctccca aatgggggga gccggagctc ctttcgacgg cggcggtgct tgagaagaag
     3781 ctcggcaagg caaaaaccgc tccgtgcgat tgggcggaaa cctccgcgga gagggcggca
     3841 aacgccaaaa ccgccggttc ggaaaggggg ggcgtcagat gaaatatacc taccaaccgg
     3901 tcatcggtct tgagattcac gtccagcttg cgacagcaac gaaaattttc tgctcctgtt
     3961 ccacggacta catcggagcg gagccgaata cgaacgtttg ccccgtctgc ctcggcctgc
     4021 cgggagcgct ccccgtgctc aacgggaagg ctgtggaact cgggatcaag gcagccatgg
     4081 cgctctcctg tacagtcaag gacaagacgg tctttcacag aaagaactac ttctaccccg
     4141 acttgccgaa agcatatcag atcagtcagt atgaccttcc tctggcggag gggggggagc
     4201 tcttcatctc cggggacggc gatacgctga agcgaatacg gatcacccgc ctgcatcttg
     4261 aagaggatgc cgggaaactg gtgcattcgg cggccgacgg ccgactcgaa ggggctgagt
     4321 tctcttttgt cgactataac aggggcggcg ttccgctggc tgagattgtt tcggagcctg
     4381 atcttcgttc gcccggcgag gctcgcgagt acgtgacttc gcttcgcagg cttgttcgtt
     4441 atctcggcgt ctccgacggc gacatggagc gcggttccat gcgcgtcgac gcgaatattt
     4501 cgctgcagaa gacagacgcc gcgacaggtg agattctcta ctggggagag cgcgccgaga
     4561 taaaaaatat gaactcgctt cgcgcgatag aacgcgccct ggaatacgag attcgcaggc
     4621 aaatcgacgt catggaagca ggaggcgttc tcgaacggga aacgaggcac tggaacgacg
     4681 gagaaggaac gaccacctcg atgcgaagca aggaggcggc ccacgactat cgatatttcc
     4741 ctgatcccga tctgcctcct atcgaagcgt ctcccgcact attggatcag gtgagcgcat
     4801 cccttcccga aatgccgtgg gccagagagg cccgcttccg caacgagttg ggccttcccg
     4861 aagcggatgc cgctctcctc gcggagacgc cgcagcttgc ggactacttc gagagctgcg
     4921 tcctgaccgg cgcctctccc tccaggattt cgaactgggt tcgtactgaa gtgctccgtg
     4981 ttctgaacga acgaaacata tccatcgact ccttctctat gcagccgaaa actctcggtt
     5041 ccctggtgct tctggtggac gaggggaaac tgtcgactac tgctgcaagg gagactttca
     5101 acgcaatgat agaaaaaggg ctccctctcg aaaaggcgct ggaggcgagc ggaggcgcgg
     5161 gagggaaggt ctccggagac gcgcttgccg ataccatccg ggctgtgctt gcatcggctc
     5221 ccgacgtcgt ggaggaaatc ctcggaggca aagacgataa gggcaagaag aagaaatttt
     5281 taaccggact cgttatgaag gagactcgcg ggcaggcgga tgcagccgcc gtctgggagt
     5341 tgatcgagaa attgttgaat gaaaaaccct gaaacattcg tcttctctca aggggtgtat
     5401 tttaccagct tttgaaaaga gatttttttg attcgttgac taatggaggc attttgcgta
     5461 agatattcaa gttgccctgc agtagagcag aggcaggaac gaaacgccgg aggaggtagt
     5521 tggcaatggg aacccgtcag cctgagaaca ctcgcagttt cgcaatcgcg gcccatggag
     5581 gcgcaggaaa gaccagtctc gttgaggcca tgcttttcga tagcggcagt acgtccagac
     5641 tgggaaaggt cgaagacggc aacaccgtca gcgatttcag tcaggaggaa caaaagcggc
     5701 agatctcgat caacacatct cttatcacta tggagaggaa tgggaagacc ctctttgtcc
     5761 tggatactcc gggatttgct gatttcgtag gagagatgcg ctcggccgta cgcgtcgccg
     5821 atacccttgt catcgccgtg agcggaacga gcggcatcga agtgcagacc gacaaggcct
     5881 ggcagacggg agaggagttc gggcttcccg tggctttcta catctccaag ctggaccgtg
     5941 agaatgcgga tttcggcaag gtgctctccg atatcaaggc ggtgtacagc gagaaagccg
     6001 ttcccgtctt ccttccgata ggaagcgagt cgtccttcaa aggcgtcgtc gatattctcc
     6061 gcagcaaggc gtatatatat gaaatcaacg gaagcggaaa gttttccgag ggcgatgtgc
     6121 ccgccgacat ggcggccgac gttgcttcgg ccagagaaac gctggtagaa acagtagtgg
     6181 aagccgacga cgagatgatg atgcgctatc ttgacggaga gtcgatctcc gacgacgagc
     6241 tgcttccagt tcttcggaaa gcgattgcct cacgtcttct gctccctgtt ttccccggct
     6301 catccacgca gaatgtgggc gtgcatcaga tactcgactt catcgtcgac tttttcccgt
     6361 ctccgctcga agcccgtacg cgtcccgcga agaagggcga caaagacata gaagtcgctc
     6421 cggacccgtc cgccactttc tccgccatct gcttcaaagt catggtggac ccatacgtcg
     6481 gcaagctctc tttcatccgc gtcaactccg gaaccctcac ttccgataac aacattttca
     6541 acgtcaacaa gggcgaagac gagcgtatca gctccttcaa gttcatgacg ggcaaggaag
     6601 gcaaagatgt taaggagatt gtcgtcggcg acattctcac gctgccgaag cttcaaagcg
     6661 tccaggttgg cgacacgctt ggcatgaaag gctcgacggt aatatatcct cccatcgact
     6721 ttccaaaacc cgtctatagc gtcgcgatca cggcaaagag ccgcgccgac gaggacaagc
     6781 tgggcaatgc gatcaataag actcttgagg aagatcccag cctcagcttc gagaagaacg
     6841 cggagacaaa cgacagcgta ctctcaggca tgggggatat gcatatcgat atcgtcctct
     6901 cgaagatgaa agagcgctac ggcgttgatc ttgaaaccaa gacgccccag gtaccctacc
     6961 gcgagaccat ccgcaaaact gccgaggccc aggggaaaca caagaaacag accggcggtc
     7021 acggtcaata cggagacgct catatccggt acactccgct cgaaagaggc gcaggatttg
     7081 tgttcgagga caatattgtc ggcggcgcca ttccaaaggg attcatcccc gcagtcgaga
     7141 aagggctccg tgagtcgttg gtcaagggac cgcttgcagg ttttcctgtg gtcgacttca
     7201 aggcgacgct cttcttcggc tcgtatcacg acgtagatag ctccgaaatg gcgtttaaac
     7261 tcgccgcccg cctttccttt aagaagggta tccttgatgc gggccccatt cttctcgagc
     7321 cgatcatgaa tgtcgaagtc accgtgccgg aggactacct cggagacgtt atgggcgact
     7381 tcaacagcag gagaggacgt atcctcggca tcgacagcaa gggacaccag cagatcgtta
     7441 aagcgcaggc tcctcttgc
//
LOCUS       DAOPKU010000080         7455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10567_length_7455_cov_4.557162, whole genome shotgun sequence.
ACCESSION   DAOPKU010000080 DAOPKU010000000
VERSION     DAOPKU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10567_length_7455_cov_4.557162"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /locus_tag="PK849_03735"
     CDS             complement(<1..2134)
                     /locus_tag="PK849_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="HPE65268.1"
                     /translation="MPDRPAAMKTPWKNRRVATPTMLQMEATECGAASLGIVLGYYGL
                     HLPLETLREECGVSRDGSRATNVLKAARRHGMEAKGYRKEPQTLGDLPLPIILFWNFN
                     HFLVLEGIKGDKVYLNDPAAGPRTVDRETLDRSFTGVAISIVPGETFRPEGRPRSPWA
                     ALARRLVGARAALLFAVLAGLALVIPGLMEPAFQQIFVDRLLIEGRLEWFRPMLTLMA
                     IVALVKGALTWLQGSVLLRQELAMAIRDSASFMNHLLKLPYTFFLQRYAGEIGNRVQL
                     NDRVAQLLSRELATTVLQCILVLFYAVMITLYDPPVSVIALGAAGACALLIVSVNRRR
                     TDLNERFTGEVGKFAGAAIGGLSVIETIKSFGGEEDQFARLAGYLSKIADCRIELSRL
                     AVRIQAVLPTLTALAAAGVLCFGGFRVMDGVLTMGMLLALQTLMTQTINPVTELVTLW
                     DSFQTAQGNLNRLDDVLRYPRMPERPALEDGAMASSKQAGARTVRLSGRLELRDVSFG
                     YSKLGEPLIQDFSLTLVPGSRVALVGGSGSGKSTVARIVCGLFEPWSGDVLLDGRLLA
                     TISREETATSFAFVDQEISLYEGTIRENLSLWDPSVPTEQLVAACRDAAIHKDVMARQ
                     GGYDAIVEENGRNFSGGQRQRLEIARALVRSPSLIVLDEATSALDAPTEEAIDRALRR
                     RGCTCLIVAHRLSTIRDCDEIIVLHQGKV"
     gene            complement(2127..3422)
                     /locus_tag="PK849_03740"
     CDS             complement(2127..3422)
                     /locus_tag="PK849_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin system secretion protein"
                     /protein_id="HPE65269.1"
                     /translation="MKKQIFRQSALDRLSSPEQLDMLFQVVRPAYWAALSALLLLLTV
                     LVVWGFYGTVTTMVSGQGILLREGARVDASSPGSGRLEVIFVDIDDPVEKGHVIARLA
                     QPDLRNQIAEMRGRVEILRAERAMGTEMGVDKKTLESEFLARRRQALNSSIKVARDRA
                     ESLREQLEQYDALYAKKYITRSQYLDVKDRYNAALQEILSFNEELARLPMTAADTTSQ
                     IERQRIDVDMRLLTAEKQLDALEERYDLETSVRSPETGRVLEIFKLEGQVISAGEALL
                     SLEKSDDSGAPLYVHAYVQPQDGKKILPEMEAQISPSVVKQEEFGVLRGRVTQVSSFP
                     ASARGMTRVLGNEQLVQTLSRGGAPIAVTMALSPSDRTESGYVWSSTDGPPLTLQSGT
                     LCGVTVVVRRQAPATLVLPFLKKFFLGVGEERDGGTKDA"
     gene            complement(3573..3836)
                     /locus_tag="PK849_03745"
     CDS             complement(3573..3836)
                     /locus_tag="PK849_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HPE65270.1"
                     /translation="MSMESAVAFYERMEKDVELQRKLKELGSKERVQPYLKNELGYDF
                     TGEEMRKVILDKNPEMTDGDLEEVTGGAHYPIFGEISAIITGF"
     gene            complement(3939..4352)
                     /locus_tag="PK849_03750"
     CDS             complement(3939..4352)
                     /locus_tag="PK849_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65271.1"
                     /translation="MSKENVRKFYEVLSKDADLQRRFREKNELLSGKYRDGAPGDERL
                     EGLFLKEILPVAREAGFEFSFQELQEYSADSKMTGELMDEELAAVAGGGDLCVCVVGG
                     YGTIGDVAIGCAMYGGAESSTFFCFCFMGGGGGLR"
     gene            complement(4385..5809)
                     /locus_tag="PK849_03755"
     CDS             complement(4385..5809)
                     /locus_tag="PK849_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE65272.1"
                     /translation="MYCTLKERYRLRGWEQLPYAIQDVASGGVVFLDEKAFQAASFCN
                     GMVDVASPLLLPAHRRAVSLLKEQGMVEECAFGEGLKEEQKYRLIPCRYMRKVHWSIT
                     GRCNLKCRHCFMSAPHAKYGELSLEQSLNIVRQISEAGILDVSLTGGEPLVRNDFFDI
                     VDALLDERIMISQVYTNGVLVDEALLNKFEAQGIRPEFLLSFDGIGCHDWLRGVPGTE
                     RQTIDAIRLLRSRNFTVSIETALYRGNLHTLPTSLELFAGLGVESWKATPATDAGEWL
                     NEKGRYTLSVDELYEAYLDFIPHFKAAGAPLTLMLGGFFLCRKGEESYRIPCKKYDGS
                     GRMLRQTVCGSARNTMYISADGKLLPCIPLSGMPIQDEMPDITKTPLCEALSDSKYLS
                     LIDTRLEELLKENERCASCEHRLSCGGGCRAGSLAAGNEYLGVDESTCAFFKNGYEER
                     IRVAWGGNDASGCAPGCGDFRRCT"
     gene            6195..7439
                     /locus_tag="PK849_03760"
     CDS             6195..7439
                     /locus_tag="PK849_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE65273.1"
                     /translation="MKKRPLLYVVFLLPLILPCAAFSAHMDEMQYLALVMERNNDLAA
                     ARQEIEARLFSARAELAIQRPSLSLSAETSKWLDKNRGDQQADLSLSHRVNLSGAYGL
                     QERDLLLGVEILRNQYADAVNERLAFAASAYRQAVMAALNREAMEQIVEKRRESLTIT
                     KEKFDKELVSLLELLRAQSQVDDGEAFLLQARQKYEQQLVEMSALAGGAPAIPSVALP
                     ELGGMNLVVDEEKAWDNRPDVAALLLSKERASVQRSLAAKGLSPFVDLSVGLRVLEDH
                     IFPQERGGEVFVRAVLTVPLADGGKTSNSTEAATLMLRKAEYELAARRDSLKREADMV
                     RERWNRALEIVAIRRRQAERADKELEIAELMYREGMGSQLDLINAQEADQKSRTEFLS
                     AVQELWLVLAEADRVMGRWSQR"
BASE COUNT         1451 a         2484 c         2154 g         1366 t
ORIGIN      
        1 cgaccttccc ctgatggagc acgatgatct cgtcgcagtc gcggatggtg gacaggcggt
       61 gcgcgacgat caggcaggtg cacccgcgcc gccgcagcgc ccggtcgatg gcctcctcgg
      121 tgggggcgtc cagggcgctt gtggcctcgt cgagaacgat cagcgaagga gagcgcacga
      181 gcgcgcgggc gatctccaac cgctgacgct gccccccgct gaagttgcgc ccgttctcct
      241 ccacgatcgc gtcgtacccg ccctgccgcg ccatgacgtc cttgtgaatg gccgcgtcgc
      301 ggcacgccgc cacgagctgc tccgtcggca cggagggatc ccagagcgag agattttccc
      361 gtatcgtccc ctcgtagagg gaaatttcct ggtcgacaaa ggcgaaggag gttgccgtct
      421 cttcccgcga tatcgtcgcc agcaggcgac cgtcgagcag cacatccccc gaccacggct
      481 cgaacaggcc gcagacgatc ctcgccaccg tggacttgcc cgacccggaa ccgcccacaa
      541 gcgccacgcg ggaaccggga acgagcgtca gcgaaaaatc ctggatcagc ggctcgccga
      601 gcttggagta gccgaaggag acgtcgcgca actcaagccg ccctgagaga cgcaccgtac
      661 gcgcccccgc ctgcttcgac gaggccatcg ccccgtcctc cagcgcgggt cgctcgggca
      721 tacgtggata gcggagcaca tcgtcgaggc ggttcaggtt cccctgcgca gtctggaagg
      781 agtcccaaag agtcaccagc tccgtcaccg ggttgatcgt ctgcgtcatc agcgtctgca
      841 gcgcaagcag catgcccatg gtgagcacgc cgtccatcac cctgaagccg ccgaagcaga
      901 gcacccccgc cgcggcgagg gccgtcagcg tcggcaggac agcctgaatg cgcaccgcaa
      961 gacgggaaag ttcgatacgg cagtccgcga tcttgcttaa atacccggcg agccgggcga
     1021 actggtcctc ctccccgccg aaggatttga tcgtctcaat gacggagagg ccgccgatcg
     1081 ccgctccggc gaactttccg acctcgccgg tgaagcgctc gttcagatcg gtccgccgcc
     1141 tgttcaccga gacgatgagc agcgcgcagg cccccgcggc gccgagagcg atcacggaga
     1201 ccggaggatc gtagagggtg atcatcacag cgtagaagag cacgaggatg cactggagca
     1261 ccgtcgtcgc cagctcgcgg gagagaagct gcgccactct gtcgttgagc tgcacccggt
     1321 tgccgatctc cccggcgtac cgttgcagga agaacgtata ggggagcttc agcagatggt
     1381 tcatgaagga cgccgaatcg cggatcgcca tcgccagctc ctgccgcagc agcaccgaac
     1441 cctggagcca cgtcagcgcg cccttaacga gcgcgacgat cgccatgagg gtcagcatcg
     1501 ggcggaacca ttcgaggcgt ccctcgatga gaaggcggtc gacgaagatc tgctggaagg
     1561 cgggctccat gaggccggga atcaccagcg caagcccggc gagcacggcg aagagcagcg
     1621 ccgcgcgagc gcccacaagc cgtcttgcga gcgcagccca cgggcttcgc ggacgtccct
     1681 ccggacgaaa ggtttctcct ggaacgatgg agatggcaac accggtgaac gaacggtcca
     1741 gagtctcccg gtccaccgtg cgcggtccgg ccgcggggtc gttcaggtag actttatcgc
     1801 ccttgatgcc ttcgagcaca aggaagtgat tgaagttcca gaaaagaatg attgggaggg
     1861 ggagatcgcc cagggtctgc ggctccttgc ggtacccctt cgcttccatc ccgtggcgcc
     1921 gcgccgcctt caggacgttg gtcgcccggc tgccgtcgcg gctcacgccg cactcctcgc
     1981 gcagcgtctc aagaggcaga tggagcccgt aataccccag aacgatcccc agcgaggccg
     2041 cgccgcactc ggtggcctcc atctggagca tcgtcggcgt cgcgacgcgc ctgtttttcc
     2101 agggcgtctt catcgccgcc gggcgatcag gcatctttcg ttcctccgtc gcgttcctca
     2161 ccgacgccga ggaagaactt cttcaaaaac gggaggacga gcgtcgccgg agcctgacgg
     2221 cgcaccacca ccgtcacgcc gcaaagagtg ccgctctgga gggtcagcgg gggcccgtcc
     2281 gtggaggacc agacgtagcc gctctccgtc cggtcggacg gggagagggc catcgtgacg
     2341 gctatcggcg cgccgccgcg cgagagcgtc tgaacgagct gctcgttccc cagcacccgc
     2401 gtcatgcccc tcgcggacgc agggaatgag gagacctgcg tcacccgtcc gcgaaggacg
     2461 ccgaactcct cctgcttcac cacggacggg gagatctgcg cctccatctc cggcaggatc
     2521 ttcttgccgt cctgcggctg aacgtaggcg tgcacgtaca gcggcgcgcc ggagtcgtcg
     2581 ctcttttcaa gcgagagcag agcttccccc gcggagatga cctgcccctc cagcttgaaa
     2641 atttccagca cgcgcccggt ctcggggctg cgaacggaag tctccagatc gtagcgctcc
     2701 tcgagcgcgt caagctgttt ctccgccgtc agcaggcgca tgtccacgtc gatccgctgc
     2761 cgctctatct gcgaagtagt gtcggcggcg gtcatcggca gccgggccag ctcctcgttg
     2821 aaggagagaa tctcctgaag cgcggcgttg taccggtcct tcacatccag atactgactc
     2881 cgcgtgatgt acttcttcgc atagagagcg tcgtactgct cgagctgctc gcggagcgac
     2941 tcggcgcggt cgcgcgccac cttgatcgaa gagttcagcg cctgtctgcg ccgcgcgagg
     3001 aactccgact ccagcgtttt tttatcgacg cccatctccg tccccatcgc ccgctcggcg
     3061 cgcagaatct ccaccctgcc ccgcatctcg gcgatctggt tccgcagatc gggctgggca
     3121 agccgggcaa tcacatgccc cttctcgacg ggatcgtcga tgtcgacgaa aataacctcc
     3181 aggcggccgc ttcccggaga cgaggcgtcg acgcgcgcgc cttcccgcaa cagaataccc
     3241 tgcccggaga ccatcgtcgt cacagtgccg tagaaccccc agacaaccag gacggtcaac
     3301 aggagcagca gtgcggagag cgccgcccag tacgcgggcc ggacgacctg aaagagcatg
     3361 tcgagctgtt cgggggagga aagcctgtcg agagccgact ggcggaaaat ctgtttcttc
     3421 acgatcgggc acgctccttc aggaaaaatc agaactgacg tatcacggta tacgcgggag
     3481 aattcctact gcacagaatt atacaccacc gcttcaatga gggaaacggg cattccagcg
     3541 tattggaagc gccacctccc ccgggaaagc gatcagaatc cggtaatgat ggcggatatc
     3601 tcaccgaaaa tcgggtaatg cgctcctccg gtcacctcct ccaaatcgcc gtcggtcatc
     3661 tcgggattct tgtccaaaat caccttccgc atctcctccc cggtgaagtc atagcccaac
     3721 tcattcttga ggtatggctg caccctctcc ttgcttccaa gctccttcaa ctttcgctgc
     3781 aattcgacgt ccttctccat tcgctcgtaa aacgcaactg cggattccat cgacatctac
     3841 aatccctcct tcaaattcgt ccatcccttg cggaacggac gttcccctga cttctccgaa
     3901 tccctcctcg taatcaactt ttcaaggccg ttgcgatgct atctgagccc tccgcctcct
     3961 cccataaagc aaaagcagaa aaatgtagat gactcggccc ctccatacat cgcacatccg
     4021 atcgccacgt ctccaatagt accgtatccg cccactacgc agacgcacag gtcccctccc
     4081 ccggcgaccg cggccagctc ctcgtccata agttcgcccg tcattttaga atcggccgag
     4141 tactcctgca gctcctgaaa cgagaattca aacccagctt cccgggcaac aggaagaatt
     4201 tctttcagga acagcccctc caaacgttca tcgccgggcg cgccatctcg gtactttcct
     4261 gagagcaatt catttttctc cctgaatctc cgctgtaaat cagcatcctt cgacaacact
     4321 tcatagaact tccgcacgtt ctccttcgac atctccagtc acctctttct gaacgatgtt
     4381 cgtttcatgt gcacctccgg aaatcaccgc agccgggcgc gcatccggat gcgtcgtttc
     4441 cgccccaagc aacccgtatt ctctcctcgt acccgttttt gaaaaaggcg catgtcgact
     4501 cgtcgacccc gagatactcg ttccctgccg cgagggagcc cgcgcggcaa cctccgccgc
     4561 aggaaaggcg gtgttcacag gaagcgcacc gctcgttctc cttcaacagc tcctccaacc
     4621 gcgtgtcgat caggctcagg tacttcgagt cggaaagcgc ttcacagagc ggcgtcttcg
     4681 tgatatcggg catctcgtcc tgaatgggca tgccggagag cggaatgcac ggcaagagct
     4741 tcccgtccgc cgagatgtac atggtattcc gggcgctccc gcacacagtc tgccgcagca
     4801 ttcttccgct tccgtcgtac tttttgcagg gtatccgata ggattcttcg cccttgcggc
     4861 acaggaaaaa cccgccgagc atcagcgtca acggcgctcc cgccgccttg aagtgcggaa
     4921 tgaagtcgag gtaggcctcg tacagctcgt cgacggagag cgtgtacctc cccttctcgt
     4981 tcagccattc gcccgcatcg gtcgccgggg tcgccttcca actctccacg ccgagcccgg
     5041 cgaaaagctc caggcttgtc ggcagtgtgt gcagattccc ccggtagagc gccgtctcga
     5101 tggagacggt aaaatttctc gaacgcagca gcctgatcgc gtcgatcgtc tgcctctccg
     5161 tcccgggcac gccgcgcagc cagtcgtggc agccgatgcc gtcgaaactg agcaaaaact
     5221 ccggacgaat tccctgggcc tcgaacttgt tcaggagcgc ctcgtccacc agcacgccgt
     5281 tggtgtagac ctgtgagatc atgatgcgct catcgagcag ggcatccacg atatcgaaga
     5341 aatcatttct gaccagcggc tcgcctccgg tcagcgacac gtccaggatg cccgcttcgg
     5401 aaatctgccg gacaatgttc aggctctgct ccagcgacag ctccccgtac ttcgcgtgcg
     5461 gcgcggacat gaagcagtgg cggcacttca ggttgcatct ccccgtgatc gaccagtgga
     5521 ccttgcgcat gtacctgcac ggaatcagcc tgtacttttg ctcctccttc agcccttcgc
     5581 cgaaggcgca ctcttcgacc atcccctgtt ctttcagcag cgagacggcc cggcgatgcg
     5641 cggggagcag cagcggagag gccacgtcca ccatgccgtt gcagaatgat gcagcctgga
     5701 acgccttctc gtccagaaag acgacgccgc cactcgcaac atcctgaatc gcgtacggca
     5761 gctgttccca ccctctgagc ctataccgtt cctttagcgt gcaatacacc gcctcatcct
     5821 ctcccctcgc gttccatcat cttcttcggt tcaggtactg gacgacgacc gaaagatcgc
     5881 cccgggacga cgaaagacgg aaaggctttg catcgtcgac ttcagacgca gcatacacct
     5941 ttacacagtg tcaaagtcaa gcggcatttt tcaaaagagt cgcaacctgc accgaattct
     6001 atcatccgac ggagaaaaaa gggggcggtt tattttctcc ccctcggaaa cgcagtatcg
     6061 acaagtcttt gtagaattac cgaaagaatg ctttccattc ggcccgaatg agcaacgtat
     6121 ggttttcgac gggccgctgg ggtagaatga tcgcagtgaa cgactattca ttcgatccat
     6181 ttcaaggagg gtccatgaaa aaacgcccgc tcctctatgt agtgtttttg cttccgctca
     6241 ttcttccctg cgccgccttt tccgcgcaca tggacgagat gcagtatctt gcgctcgtga
     6301 tggagcggaa caacgacctc gccgccgcca ggcaggagat cgaggcgcgc ctcttctccg
     6361 cccgggcgga actggccatc cagcggccgt cgctttcgct ctccgccgag acgagcaagt
     6421 ggctcgacaa gaaccggggc gaccagcagg ccgatctctc cctctcgcac cgcgtcaacc
     6481 tctccggagc ctacggcctc caggagcggg atctgctgct cggcgtcgag atccttcgga
     6541 accagtacgc cgatgcggtc aacgaacgcc tcgccttcgc cgcgtccgcc taccgccagg
     6601 cggtgatggc cgcgctgaac cgggaggcga tggaacagat cgtggaaaag cgccgcgagt
     6661 cgctcacgat cacgaaggag aagttcgaca aggagctggt ctccctgctg gagctgctgc
     6721 gggcgcagtc gcaggtagac gacggcgaag ccttcctcct ccaggcccgg cagaagtacg
     6781 agcagcagct cgtggaaatg agcgctctcg ccggaggggc tccggcgatt ccgtcggttg
     6841 cgcttcccga actcggcgga atgaatctcg tcgtggacga ggagaaggcg tgggacaacc
     6901 gccccgacgt cgccgccctg ctgctctcca aggaacgggc ttcggtgcag cgctcgctcg
     6961 ccgcaaaggg gctctcaccg ttcgtcgacc tctccgtcgg gctgagggtt ttggaggacc
     7021 acatttttcc ccaggagcgg ggcggagaag tcttcgttcg cgccgttctc acagttcctc
     7081 ttgccgacgg cgggaagact tccaactcca ccgaagcggc gacgctgatg ctccgtaagg
     7141 ccgagtacga gctggccgca agacgcgaca gtctgaaacg ggaggccgac atggtgcggg
     7201 agcgatggaa ccgcgcgctt gaaatcgtgg ccatccgccg ccgtcaggcc gagcgcgcag
     7261 ataaagagct cgaaatcgcc gagctgatgt accgcgaggg aatgggctcc cagctcgacc
     7321 tgatcaacgc ccaggaggcc gaccagaaga gccggacgga gttcttaagc gctgtgcagg
     7381 agctgtggct cgtcctcgcg gaggccgacc gggtcatggg gcgctggtcg cagcggtagc
     7441 ttctttcatt tagtc
//
LOCUS       DAOPKU010000081         7452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10572_length_7452_cov_4.201433, whole genome shotgun sequence.
ACCESSION   DAOPKU010000081 DAOPKU010000000
VERSION     DAOPKU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10572_length_7452_cov_4.201433"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1925
                     /locus_tag="PK849_03765"
     CDS             <1..1925
                     /locus_tag="PK849_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPE65274.1"
                     /translation="GGASARPQISSGELYDRLLPSFASLPEACGLNRRHESGSWTMPP
                     GGSGERSVVCPSLEEASARVKGLQRRIKFWRERSEKLQEEREKLLAAKVFLDALASAG
                     LDRAKSPPGAGGGKGRPDVASGSPDAAPGSTAWLERALDTRYLSLTFGRLSTDNYKRL
                     AEMAGITPLLVFPVVTEKDYVLVAIFCSRDYHPEAEKLFRVVYLKEYVLQEILEGSGG
                     AMKEYLEEQLQRSERALRALREAPRQYLERHRKELELLYCAVYAKQRIYSLCRNRGEL
                     GDVFIVSGLVPSPTLPQVEDVIERFGPNTLLLTEKDDALKRRGRRLPTLLKNTFPVRV
                     FQEIVALYSLPSYDDIDPSPLVALSFCIFFGFMFGDVGHGLLISAGAWFLARKGILKK
                     AFSSVLQIAGFSAAAFGFLYGSIFGSEELIPALWTSPMHGINDLILASLVTGTGFLSL
                     GIALNILTLRRKGRIGKMLFDCEGLAGLLFYWTAVAAGAFAFTSGEGVPKPLLFALAA
                     LFLIIMCSSLLERLFFGRGEEDEGGVVHTFSVLHALLSFVSNTASFVRLAAFALNHAG
                     LSAAVFMLGDMVRSVPGGPVFHAAVLLAGNAVIVGLEGLIVFIQTLRLEYYEFFGKFY
                     HGGGIPFEPATWRSRE"
     gene            1988..2401
                     /locus_tag="PK849_03770"
     CDS             1988..2401
                     /locus_tag="PK849_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPE65275.1"
                     /translation="MTGCVLLIAGTLLTCGTGWYLRGKAVRRPRAVLAGFMIGLNALL
                     LSAILVSLSAGSAAAAEGAAAVEGAGLGFLGASLATGMACLGAGLAVAIVGAAALGVV
                     GEKPSMLGTTLIYLGLAEGIAIYGVIVSLLILGKL"
     gene            2415..2729
                     /locus_tag="PK849_03775"
     CDS             2415..2729
                     /locus_tag="PK849_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPE65276.1"
                     /translation="MKGFLISDNHDSLVLLRLAGIPGVVVHGEEETTTAVIDVLRNRP
                     DTGVLVMTERAAAEVPLLVKSLREREDPPLLVEIPDRHGSLREPDFLTRYIRNAIGVK
                     IE"
     gene            2726..3442
                     /locus_tag="PK849_03780"
     CDS             2726..3442
                     /locus_tag="PK849_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HPE65277.1"
                     /translation="MSNSISGDGRGRELQNPAKRAAEEQNLLEMQRTPVRDDSNRKLL
                     ALKNLLLKKGDEEREVILDSARREATAWLKEQNEGLDRMVEQIHADAVKNAEEIAKRQ
                     IARAESLRAKDRLRLQNTLLDEAMAMLQRALTSLREREDYPLVLAGLVLEAAKDLPAG
                     TAALISLAVDDAPLGDALAERVGKKRPDMTFTFDPSPVPISGGAWLSAPDGSWRAAAD
                     LREVAAELKDSLAERLLAAL"
     gene            3453..5231
                     /locus_tag="PK849_03785"
     CDS             3453..5231
                     /locus_tag="PK849_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPE65278.1"
                     /translation="MNSTGKIVAINGPVIRASGMRSFAMREMVQAGRLLLLGEIIRLD
                     GDDALVQVFEDTTGLRTGEPVTGTGGPLSMSLGPGLMGSIFDGIGRPLGKLLEREGPF
                     VTRGVSIPQLDPDAMWDVTPLGKVGDVVTPGAPLALVKETSLVEHRILTPCDIEGEIL
                     SILPPGLHKASSVVARVRDGHGRERSIPMIRKWPVRTPRPIAERLLPDEPLLTGQRII
                     DGLFPISKGGVAAIPGGFGTGKTVTQHQLAKWSDAQIVVYIGCGERGNEMTQVLEEFP
                     VLEDPRSGKPLMERTVLIANTSNMPVAAREASIYTGITIAEYYRDMGYDVALMADSTS
                     RWAEALREISGRLEEIPAEEGFPAYLPTRIAEFYERAGKVTTLGGTRGSISIIGAVSP
                     PGGDFTEPVTRHTKRFIRCFWALDQQLARSRHFPAISWIDSYSEYGDEVKSWYASHVD
                     PAWATLREKARTLLAEDDKIQQIIRLVGEDVLPDDQRLIALTASFLKNGYLQQGAFGP
                     DSYCPPKKGIAILNLLIAFHQKAEDLLSQGCPLSLLKGIDELVELTHLREIPAERLER
                     FEELKHDLFERMESINRERIVSRREV"
     gene            5235..6611
                     /locus_tag="PK849_03790"
     CDS             5235..6611
                     /locus_tag="PK849_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPE65279.1"
                     /translation="MSATEYIGVRKITGPFVLLDGIQGAGYDEIVLVSTPDGEARKGR
                     VVLVDEKATLVQVFTGTEGLVPETTSVRFLGRELEMALAPSMLGRTFSGLGEPRDGYG
                     PVLGGVRRPVRGAPINPMAREYPRNFISTGISSLDVLLTLIRGQKLPIFSGNGLPHNA
                     LAVQIATQSKLLGAENFVVVFAGIGIKNDDAAYFQSHLASEGRGGNLVLFLNLADDPV
                     IERIATPRLALTTAEYLAFDLGMHVLVILTDMTSYCEALRELSVARGEVPSRKGYPAY
                     LYSDLASLYERAGALRNSKGSVTQIPILSMPNDDITHPIPDLTGFITEGQIVLSRDLE
                     ARGIYPPVDVLPSLSRLMKDGIGKGSTREDHANLAGQLFASYSRVQEVRALAGVVGTD
                     ELSRADKASLAFGDSFERLFVNQGMEENREIGHSLDLGWKLLSMLPVQELSRLSMEEI
                     EKYIKKRA"
     gene            complement(6692..>7452)
                     /locus_tag="PK849_03795"
     CDS             complement(6692..>7452)
                     /locus_tag="PK849_03795"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65280.1"
                     /translation="LPFDLHPVPEASLNDLSRVLFESEYLPAAFAPEILEANNRTYEE
                     RLASCRMVVSPDNPVPTLVGILALGKRPRAFFPGASVQFLRIDGEEFADPVIDEENVD
                     GPLREMLSKIGQKLSAHNRVFVDIVSSPTEKRIYDYPPPALQQLLYNAVLHRTYEKTN
                     TPVRIYWFNDRIEIISPGGPYGNVTRENFGRPGITDYRNPNLAEAMKVFGFVQAFGRG
                     IAVARRALETNGSSPLEFEVDQSAVKAVVRKRQK"
BASE COUNT         1242 a         2300 c         2569 g         1341 t
ORIGIN      
        1 ggggcggcgc gtccgcgcgt ccgcagatct cctcgggcga gctttacgac cgtctgctcc
       61 cgtccttcgc ctccctgccg gaagcgtgcg gactgaaccg gcggcacgag agcggctcgt
      121 ggacgatgcc gcccggcggc tccggcgagc gcagcgtcgt ctgtccctct cttgaagagg
      181 cgtcggcccg cgtcaagggg ctccagagga gaatcaaatt ctggcgggag cgttcggaaa
      241 agctgcagga agagcgtgag aagctcctgg ccgcgaaggt ctttctggac gcgctcgctt
      301 ccgcagggct cgacagggcg aagagccctc cgggcgcagg gggcggtaag ggacgtcccg
      361 acgtcgcctc cgggtctccg gacgccgcgc ccggctcgac ggcatggctg gagcgggcgc
      421 tcgacacgcg ctatctctcc ctgaccttcg gacgcctgag cacggacaac tacaaacgtc
      481 tggcggagat ggcgggaatc acgccgctgc tggtctttcc ggtggttacg gagaaggact
      541 acgtgctggt ggccatcttc tgctcgcgcg actaccaccc ggaggcggag aagctgttcc
      601 gcgtggtcta cctgaaggag tacgtcctgc aggagatcct cgaaggctcc ggaggcgcga
      661 tgaaagagta tctcgaggag cagctccagc gttcggagcg tgcgctccgc gcactccgcg
      721 aggctccgcg gcaatatctg gaacgccaca gaaaagagct cgaactgctc tactgcgccg
      781 tgtatgcgaa gcagcggatc tactccctct gccggaaccg cggcgagctg ggggacgtgt
      841 tcatcgtttc gggcctcgtt ccctccccga cgctgccgca ggtggaggac gtcatcgaac
      901 gcttcgggcc gaacaccctt ctgctgaccg agaaggacga cgcgctgaag cgccgggggc
      961 gcaggcttcc cactcttctg aagaacacct ttcctgtacg ggtctttcag gagatcgtgg
     1021 ctctctacag cctcccctcc tacgacgaca tcgacccttc gccccttgtg gcgctctcat
     1081 tctgcatctt tttcggcttc atgttcggcg acgtcggaca cggcctcctg atctccgcgg
     1141 gcgcctggtt cctcgcccgg aagggcattc tgaaaaaggc gttctcgtcg gttctgcaaa
     1201 tcgccggttt ttccgcggcg gcattcggct ttctctacgg gagcatcttc ggctccgagg
     1261 agctcatccc cgctctctgg acatccccca tgcacggcat caacgacctg attctcgcct
     1321 ccctcgtcac ggggacgggc ttcctgagcc tcgggatcgc gctcaacatt ctgacgctgc
     1381 gccgcaaggg gcggatcggg aagatgctct tcgactgcga gggactggcg ggactgctct
     1441 tctactggac ggcggtcgcc gcgggggcgt ttgccttcac ctccggcgaa ggcgtcccga
     1501 agccgctgct cttcgcgctg gcggcgctct ttctgatcat catgtgcagc tcgctgctgg
     1561 aacgcctctt cttcgggcgc ggcgaggagg acgaaggggg cgtggtgcat acgttcagcg
     1621 tcctccacgc gctgctctcg ttcgtcagca acacggcgtc cttcgtgagg ctcgcggcct
     1681 tcgcgctgaa ccacgccggg ctttctgcgg cggtcttcat gctgggcgac atggtccgct
     1741 cggtcccggg aggcccggtc ttccatgcgg cggtgctgct ggcgggcaac gcggtgatcg
     1801 tggggctgga ggggctgatc gtcttcatcc agacgctgcg gcttgagtac tacgagttct
     1861 tcggcaagtt ctatcatgga ggcggcattc cgttcgaacc ggcgacatgg agatcccgcg
     1921 agtaagaggg cgccgcgttt ccggcgccga acgatttttt acacgatctt ttcaaggagg
     1981 gttttttatg actggatgtg tgcttttgat cgcggggacg ctgctgacct gcgggacggg
     2041 gtggtatctg cgggggaagg cggtgcggcg gccccgggcg gtgctcgcgg gattcatgat
     2101 cggtctgaac gcgctgctgc tgtcggcgat tcttgtctcc ctttccgcag ggagcgccgc
     2161 cgcggccgaa ggggcggcgg cggtcgaggg ggccgggctg ggctttctcg gcgcgagcct
     2221 cgcgacggga atggcctgcc tgggagcggg actcgccgtg gcgatcgtcg gcgcggcggc
     2281 gctcggcgtg gtgggcgaaa agccctccat gctgggtacg acgctgatct acctcggcct
     2341 tgccgaagga atcgccatct acggagtcat cgtctccctt ctgattctgg gcaagctgta
     2401 acgcggcgcc ggcgatgaaa ggctttctca tcagcgataa ccacgactct ctggtgctgt
     2461 tgcgtcttgc cggcattccg ggcgtagtgg tccacggaga ggaggagacg acgaccgcgg
     2521 tgatcgacgt cctgcgcaac cgcccggata cgggggtgct ggtcatgacg gaacgcgcgg
     2581 ccgccgaggt tccccttctg gtcaaatcgc tccgggagag agaggacccg ccgctcctgg
     2641 tggaaattcc cgacaggcac ggcagcctga gggaacctga ttttctgacg cggtacattc
     2701 gcaacgcgat tggagtgaag atagaatgag caattccatt tccggagacg gaagggggcg
     2761 ggagctgcag aaccccgcca agcgggcggc cgaggagcag aatcttctcg agatgcagcg
     2821 gacgcccgtt cgggacgatt ccaaccgcaa gctgctcgca ctgaagaacc tgctgctgaa
     2881 gaagggcgac gaggaacggg aggtcatcct ggattcggcg cggcgggaag cgacggcgtg
     2941 gctgaaggag cagaacgaag ggctggaccg catggtggag cagattcacg ccgatgcggt
     3001 gaagaacgcg gaggagatcg cgaaacggca gatcgcgcgg gccgagtcgc tgcgggcgaa
     3061 ggatcgcctt cgtcttcaga acacgctgct cgacgaggcg atggcgatgc tgcagagggc
     3121 gctgacctcg ctgcgcgagc gggaggacta tccgctggtg ctggcggggc tggtgctgga
     3181 ggcggcgaag gatcttccgg cggggacggc ggcgctgatt tcgctcgcgg tcgacgacgc
     3241 ccctctgggc gacgcccttg cggaacgggt cggaaagaag cgccccgaca tgacgttcac
     3301 cttcgacccg tcgccggttc ccatttccgg cggcgcgtgg ctttccgctc cggacggctc
     3361 gtggcgggcc gcggcggatc tgcgcgaggt cgccgcggag ctgaaggact ctctcgcgga
     3421 acggcttctc gcggcgctgt gaggggcggc agatgaacag caccggaaag atcgtcgcga
     3481 tcaacggccc cgtgatcagg gcgtcgggaa tgcgctcctt cgccatgcgg gagatggtgc
     3541 aggcgggacg gctcctgctc ctgggagaga tcatccgtct ggacggggac gacgcccttg
     3601 tccaggtctt cgaggatacg acggggctcc ggacgggcga gccggtgacc ggaacgggag
     3661 gaccgctctc gatgtcgctg gggcccggcc tgatgggctc gatcttcgac ggcatcggcc
     3721 gccccctggg gaagctgctg gaacgggaag gtccgttcgt cacgcgcggc gtttcgatcc
     3781 cgcagctcga tcccgacgcg atgtgggacg tgaccccgct ggggaaggtc ggcgacgtgg
     3841 tgacgcccgg cgctcccctc gctttggtca aagagacgtc gctggtggag caccgtattc
     3901 tgacgccgtg cgacatcgaa ggagagattc tctccatcct cccccccgga ctccacaagg
     3961 cgtcgtccgt ggtcgcgcgc gtgcgcgacg ggcacgggag ggagcggagc atccccatga
     4021 tccgcaagtg gccggtgcgc acgccgcgtc cgatagcgga gcgcctgctg cccgacgagc
     4081 cgctcttgac cggacagcgg atcatcgacg ggctcttccc catatccaaa gggggggtcg
     4141 ccgccattcc gggcggcttc ggcacgggga agacggtgac gcagcaccag ctcgccaagt
     4201 ggagcgacgc gcagatcgtg gtgtacatcg gctgcggcga gcgcggcaac gagatgacgc
     4261 aggtgctcga ggagtttcct gtgcttgagg atccgcgctc ggggaagccg ctgatggagc
     4321 gcaccgtcct gatcgcgaac acgtcgaaca tgccggtggc ggcgcgcgag gcgtccatct
     4381 acacggggat caccatcgcg gagtactacc gcgacatggg gtacgacgtg gcgctgatgg
     4441 ccgactccac ctcccgctgg gccgaggcgc tccgcgagat ctccggacgc cttgaggaga
     4501 taccggcgga agagggcttc ccggcctacc ttcccacgcg gatcgcagag ttctacgagc
     4561 gcgccggcaa ggtgaccacg ctgggcggca cgcgggggag catctcgatc atcggcgcgg
     4621 tctcccctcc ggggggcgac ttcaccgaac cggtgacgcg gcacacgaag cgcttcatcc
     4681 gctgcttctg ggcgctggac cagcagctcg cgcggtcgcg ccacttcccc gccatcagct
     4741 ggatcgactc gtacagcgag tacggcgacg aggtgaagtc ctggtacgcc tcgcacgtgg
     4801 atccggcctg ggcgacgctt cgggaaaagg cgcggacgct tctcgccgag gacgacaaga
     4861 tccagcagat catccgcttg gtgggcgagg acgtccttcc cgacgatcag cggttgatcg
     4921 cgctgacggc gtcgtttctg aagaacggct acctgcagca gggggctttc ggccccgact
     4981 cgtactgccc gccgaagaag gggatcgcga tcctgaatct gctgatcgcc ttccaccaga
     5041 aggcggagga cctgctctcg caggggtgcc cgctctcgct gctgaagggg atcgacgaac
     5101 tggtggagct gacgcatctg cgggagattc cggcggagcg gctcgaacgc ttcgaggagc
     5161 tgaaacacga tctcttcgaa cgcatggaga gcatcaaccg ggagcgcatc gtctcccgga
     5221 gggaggtgta gaccgtgtcg gcaacggagt atatcggcgt gcggaagatc accggcccct
     5281 tcgtgctgct ggatggcatc cagggcgcgg ggtacgacga gatcgtgctc gtctccactc
     5341 ccgacggaga ggcgcgcaag gggcgcgtgg tgctggtcga cgaaaaggcg acgctggtgc
     5401 aggtcttcac cgggacggag gggctcgtcc ccgagacgac ctccgtacgc tttctcgggc
     5461 gcgagcttga gatggcgctg gcgccgtcga tgctcgggcg gacgttctcc ggactgggag
     5521 agccccgcga cgggtacggc ccggtcctgg gcggggtcag gcgtccggtg cgcggcgcgc
     5581 cgatcaaccc gatggcccgc gagtacccga ggaacttcat ctcgacgggg atctcgtcgc
     5641 tcgacgtgct gctgacgctg atccgcggac agaagctgcc gatcttctcg gggaacggcc
     5701 tgccgcacaa cgcgctcgcg gtgcagatcg cgacgcagtc gaagctgctg ggggcggaga
     5761 atttcgtggt cgtcttcgcc gggatcggca tcaaaaacga cgacgcggcg tactttcagt
     5821 cgcacctcgc ctcggagggg cgggggggca atctggtgct cttcctgaac ctggcggacg
     5881 accccgtgat cgagcgcatc gcgacgccgc gcctcgcgct gacgaccgcc gagtatcttg
     5941 ccttcgatct ggggatgcac gtgctcgtga tcctgacgga catgacaagc tactgcgaag
     6001 cgctcaggga gctgtcggtg gcgcgcgggg aggtgcccag ccgcaagggg tatcccgcgt
     6061 atctgtacag cgacctcgcc tccctctacg aacgcgccgg agcgctgcgg aacagcaagg
     6121 ggagcgtgac gcagatcccc atcctcagca tgcccaacga cgacatcacc caccccatcc
     6181 ccgacctgac gggcttcatc acggaggggc agatcgtgct ctcccgcgat ctggaggcga
     6241 gggggatcta ccctccggtg gacgtcctgc cgagcctttc gcgcctgatg aaggacggta
     6301 tcgggaaggg gtccacgcgg gaggaccatg cgaacctcgc cgggcagctt ttcgcctcgt
     6361 acagcagggt gcaggaggtg cgcgccctcg ccggagtggt gggaaccgac gagctgagca
     6421 gggccgacaa ggcgtcgctg gccttcggcg actctttcga gcgcctcttc gtgaaccagg
     6481 gaatggagga gaaccgcgag atcggccatt cgctcgacct ggggtggaag ctgctctcga
     6541 tgcttccggt gcaggagctg tcgcgcctct cgatggaaga gatcgagaag tacatcaaaa
     6601 agagagcgta gaaaaccgcc gcccccgagt gctcccgggc cgggggcggc ggttttcaga
     6661 gacgcttcgg taagacgcgt atcgggtatt cttatttttg gcgctttcgt acaaccgcct
     6721 tgactgcgct ctggtcgact tcgaattcaa gcggtgaact gccgtttgtc tccagggctc
     6781 tccgggcgac ggcaattcct cttccgaaag cttgtacaaa tccgaatacc ttcatcgcct
     6841 cggcaagatt cgggttcctg tagtcggtaa tccccggcct gccgaagttc tcccgcgtca
     6901 cgttgccgta aggccctccc gggctgatga tttctatcct gtcgttgaac cagtagattc
     6961 ggaccggcgt gttcgtcttc tcatacgttc tgtggagcac cgcgttgtac agcagctgct
     7021 gcagcgccgg cggcggatag tcgtagattc tcttctccgt aggggacgag acgatatcca
     7081 cgaacacccg gttatgcgcg gaaagtttct gcccgatctt cgaaagcatt tctctgagcg
     7141 ggccgtcgac gttttcctcg tcgatcaccg gatcggcaaa ctcttcgccg tctattcgca
     7201 gaaactgcac cgaggccccg gggaagaagg cgcgcgggcg ctttcccagg gcgagaatgc
     7261 cgacgagcgt gggaacggga ttgtcggggg agacgaccat ccggcagctc gcgagccgct
     7321 cttcgtacgt tctgttgttc gcctcaagaa tctccggcgc gaaggccgcg ggaagatact
     7381 cgctctcgaa gagcactctg gaaagatcgt tcagcgaggc ctccggaaca gggtggagat
     7441 cgaacggcag gt
//
LOCUS       DAOPKU010000082         7398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10667_length_7398_cov_5.400517, whole genome shotgun sequence.
ACCESSION   DAOPKU010000082 DAOPKU010000000
VERSION     DAOPKU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10667_length_7398_cov_5.400517"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..148)
                     /locus_tag="PK849_03800"
     tRNA            complement(62..148)
                     /locus_tag="PK849_03800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Leu,seq:taa)
     gene            complement(246..1472)
                     /locus_tag="PK849_03805"
     CDS             complement(246..1472)
                     /locus_tag="PK849_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPE65281.1"
                     /translation="MNSIEMIVVERLFEEEGILAGDILSPAGVLLLPSGLDLDSLRST
                     RPDAIELLQKHGVDKIPVKKHEPVTIEEFQRLISSFTPPIARLNPLLSRVIAHQMGAI
                     YTNIGNRELREEGVRSLFSVGATLASEVRRIPQITLSLGEDAEGKLDAAFHGVNVALV
                     AGHIATRLFPLWPDFIRSVTISGLFHDIGKAFILSSGYSADILKMLRDDRITQTHPLL
                     GEALLRDAGIRSQDILSFVRSHHEVWDGSGYPDGLTGENIPVGARILAVANVFHNVLS
                     EKGEGKKRSDQAISSLIASALGRFDHFIVRALLSSVGLFPPGSVVELSDGRNGIVLET
                     RERNLIRPRILLMTGLRKQRELAPEIVDLRITTSVSIRRAIDDFGAMPVPPLAAVGED
                     RTNVLFRKYRSPARLE"
     gene            1746..2765
                     /locus_tag="PK849_03810"
     CDS             1746..2765
                     /locus_tag="PK849_03810"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPE65282.1"
                     /translation="MRRLLDKNENPFDLPEEIKALILQKIGRASFNRYPDPEYAVLKE
                     RLSMYCGFSADSIVPGNGGDEILWMLFSRYVKPGDSVLVFSPTFSEYYRLADLFGASL
                     CTVQADLNGDEPRFDHTLFLSRMAELKPSLVLVDSPNNPTGQSHPVSFIGKAVALCES
                     TIVIDEAYGEFARDNWLSSMRGKVLPANVVVLKTLSKAWGLAGLRLGYAVCGKDSASA
                     LNAARSPFNVNILSGTAAEIILEHSEVMLARVKLLCGIRDGFISLVNRLQGWRAFSSD
                     ANFVLIRAPFPWSSFASSLGDLCLKRFALSSDTEDSTCWLRVSIGTEEDMESVISLFD
                     TMQRR"
     gene            2772..3101
                     /locus_tag="PK849_03815"
     CDS             2772..3101
                     /locus_tag="PK849_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HPE65283.1"
                     /translation="MTEKWKDKLTDQLCRALLSLETPDEVYCFLEDVATIGEIRALAQ
                     RLEVARLLSEAYTYPQIAQQTGASTATISRVKKFLEYGADGYKIVLDRLKEAGEATNP
                     EDKSSKA"
     gene            complement(3171..3875)
                     /locus_tag="PK849_03820"
     CDS             complement(3171..3875)
                     /locus_tag="PK849_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPE65284.1"
                     /translation="MTPKKNSKKEPPSRPQDIDTVFDILEELWGNEKNEPRLGHEEPL
                     DGLILTLLSQNTNDRNRDKGFAALKNSFPSWELVASASAEAIADRIRPAGLAKTKSER
                     MLQILAALKERFGEYSLMALKDMDNDAVREYLSSLPGIGAKTVACVMLFDLGMSAFPV
                     DTHIARFCRRMKWAAESLPPDEIQPLLEEWVPERRFLGGHVNIIEHGRGLCHARTPEC
                     GKCPLHSNNLCPSVQE"
     gene            complement(3856..6348)
                     /locus_tag="PK849_03825"
     CDS             complement(3856..6348)
                     /locus_tag="PK849_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65285.1"
                     /translation="MTKATTNPSRKGSKITAGSAQNKSLTIEQLRKKTDAASLGISTT
                     NEIAPLEEFLGQKRAVSSISFGLEVESKGYNIVVLGNPGSGRTTYALERLRTAARKRT
                     PPDDWVYVYNFEDPGQPLAINLPAGKGKEMGTAFDEMVEELKTSISKAFEKSQYEDAK
                     AQLVKEFQEEVNQLMDEVKAWAAENGFSLKRTPQGFVNIPLTEEETESGEKTKREIQQ
                     EEFEALSEEEQKSLQKKSEEVSQKTLETLRKIRDLEKGLKERIVKLEAEICRNAIMPY
                     LSDIKEKFGTNGDSSKDENQKSNDVLGSWIDALAEDIIDNFGMFVAAVKDENAEIDFT
                     RYTVNVFVCNDPENGAPVIWETNPTYYNLGGKVEYESRQGYLYTDFKKIVPGAFQKAN
                     GGFLVLDAEKVLLNFMSWEALKRILRTGEATIENLGEQYGAVPVSSLRPQPIPINMKV
                     VMVGTPFLHALLQYYDPEFQKMFKIKADFDTDMERTPETEKQMAQFIASCLKKENGLP
                     FDASGVAEVIDCSARIADDQGRISTEFNKITEIIVESTAWAKAENADTVQREHVKKAI
                     REKRFRSDLIQEKIARTYQDGLVRIDTSGKVVGQINGLSVIDLMDYRFGHPSRITANV
                     FMGQDGVVNIEREVKMTGPIHNKGLLILTSYMGRKYAQDMPLSLSAKLTFEQMYGGIE
                     GDSASSTELYCLLSALSEVPLKQGIAVTGSVDQFGNIQPIGGVNEKIEGFFEYCEHGG
                     LTGEQGVLIPVQNTRHLMLDDTVIEAVSKGKFFIRAVSTIDEGIEALTGVSAESIHRK
                     VKNKLKKWAKKTEKLKKGKDSEAGGDDDSEEE"
     gene            complement(6442..6969)
                     /locus_tag="PK849_03830"
     CDS             complement(6442..6969)
                     /locus_tag="PK849_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPE65286.1"
                     /translation="MPILEYGLVHVYMGHGKGKTTSALGIVLRMAGCGGKSAVVQFMK
                     GWPYSEAKGLQFLPGVTLFQTGRADYVYPDKIDPLDYSEAERGLSIGAEAVFSGEYDL
                     VVLDELNVALSFGLVPLDRVLALIGRKPPHVELVFTGRNPPQELIDRADLITEMIEIR
                     HPYQKGILARKGIDC"
     gene            7042..>7398
                     /locus_tag="PK849_03835"
     CDS             7042..>7398
                     /locus_tag="PK849_03835"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPE65287.1"
                     /translation="MLFQTRILVSGIVQGVGFRPFCVRLANSESISGYALNTSHGVEM
                     ELHGEEENLDSFLFRIVRENPPASFISSVAILDEKRKSSSPPARFEIMESRKETLQTA
                     LIPADLAVCAQCLAEMK"
BASE COUNT         1654 a         1908 c         1885 g         1951 t
ORIGIN      
        1 gaccttcggg ttatgagccc gacgagcttc ctgactgctc caccccgcga taacctattc
       61 ttggtgggag tgacgggact cgaacccgta agggccactg cccggaggat tttaagtccg
      121 ctgcgtctac cggttccgcc acactcccgc aagcgacagg agcgattcta caccaagcgt
      181 cggccactgt caatagacgg cgcgaggatc agataggaaa aaggaaaaac ataaattaat
      241 aatatttact ctaatctggc aggtgatcga tatttcctga agagcacatt cgtcctgtct
      301 tcacctactg cagcaagcgg aggaacaggc attgcgccga aatcatctat tgcacgccgt
      361 atagaaacgg aggtggtgat acggagatcg acgatttcag gagcaagctc tctttgtttc
      421 ctgaggcctg tcatcaggag gattctggga cggatgagat tgcgctcgcg agtttcgagt
      481 acgatgccgt tccggccatc cgaaagttca accacagacc ccggaggaaa aagcccgacc
      541 gaagagagga gggcgcggac gataaagtga tcgaacctgc cgagtgctga ggcaatgagc
      601 gaggagattg cctgatcgct tcgtttcttc ccttctccct tctcggagag tacgttgtga
      661 aagacattgg cgaccgcgag tattctggcc ccgaccggaa tattctctcc tgtgagtccg
      721 tcgggatagc cgctgccatc ccagacttca tgatgagaac gaacaaaaga aagaatgtcc
      781 tggcttcgga ttccggcatc ccgaagaaga gcttccccaa gaagcgggtg cgtttgggtg
      841 attctgtcgt cacggagcat ctttaatata tccgcggagt agcccgacga gagaataaac
      901 gccttcccaa tatcatgaaa aagcccgcta atagttaccg agcggataaa atccggccat
      961 aaaggaaaaa ggcgcgttgc tatgtgtcca gcaacgaggg ccacgtttac gccgtgaaac
     1021 gcggcgtcga gctttccttc ggcgtcctcg cccaatgaga gtgtaatttg cggaatacgc
     1081 cggacttctg aagcgagagt agctcctacg gaaaaaagtg aacgtacgcc ttcttcacgg
     1141 agttctctat tgccgatgtt ggtataaatg gcgcccattt gatgtgcgat gaccctggaa
     1201 agcaagggat tcagccgggc aatcggagga gtgaacgatg aaatgagacg ctggaactcc
     1261 tcaatcgtaa cgggctcatg tttcttgaca gggattttgt cgaccccatg tttttgaagc
     1321 aattcgattg cgtccggacg cgtggaacgc agagaatcga ggtcaagacc cgaggggagc
     1381 aaaaggactc ccgcaggaga aagaatatcc ccggcaagaa tcccttcttc ttcaaaaaga
     1441 cgttcgacga caatcatttc gatggagttc aattttttcc ctccttgatg cgcagtgcga
     1501 aacatgaact ggcaacgcac acaacgcgca cttatcgttg acattgacgc ggtagaatgc
     1561 tagactgcca aataaataca ttgtaacatg ctaaagtcca agtgcgcttc gagcatagaa
     1621 agagtttttg tgttttgaag cagatggcat agtacagatg ttcctggaaa ttgatatggt
     1681 attctcctct gtacgtcgta gttcaaagat tctcttgctg aaaatagaag gaggcggtga
     1741 acccattgcg ccgtttgctt gataaaaacg aaaatccgtt cgacctccct gaagagatta
     1801 aggcgcttat cctgcaaaaa atcgggcgcg cgtcctttaa tagatatccg gacccggagt
     1861 atgcagtttt aaaagagaga ctctccatgt attgtggttt ctccgctgac tctatagttc
     1921 ccggaaacgg cggcgatgaa attctctgga tgcttttttc ccgttatgtc aaaccaggag
     1981 attccgtgct cgtattttca cctacttttt cagagtacta tcgcctcgca gatcttttcg
     2041 gagccagttt atgtaccgtt caagcagact taaacggtga cgaaccgcgc ttcgatcata
     2101 cgcttttttt aagcaggatg gcggagctga aaccttcgct ggtcctcgtg gattctccga
     2161 acaacccgac gggccaatca caccccgttt cctttatcgg caaggcagtt gcgctttgtg
     2221 aatcaaccat tgtgatcgac gaggcgtacg gggagttcgc gcgggataac tggctttcat
     2281 cgatgcgagg aaaggtattg cctgcaaacg tcgttgtatt gaaaacgctt tccaaagcgt
     2341 gggggcttgc ggggctccgg cttgggtacg ctgtttgcgg aaaagattcg gcttcagcgc
     2401 tgaatgctgc ccgatcaccg ttcaacgtaa atattctttc gggaactgcc gccgaaatta
     2461 ttctggagca ttcagaagtg atgctcgcaa gagttaaact cctttgcggc atccgggacg
     2521 gatttatatc gcttgttaat cgcctccagg ggtggagagc cttttcgagc gacgcgaatt
     2581 ttgtacttat ccgagctcct tttccctgga gcagttttgc ttcttctctt ggagatttgt
     2641 gcttgaagcg cttcgctctt tcgtccgata cagaggactc aacatgctgg ttacgtgtaa
     2701 gtattggaac agaggaagat atggagtccg tcatttctct tttcgacaca atgcaaagga
     2761 ggtagaatgc tatgactgaa aaatggaagg ataagttgac agaccagctg tgccgcgccc
     2821 tcctctctct agaaacgccg gatgaggtct actgtttcct tgaagatgtc gctaccattg
     2881 gcgagatccg cgctcttgct cagcggctcg aagttgcccg gttgttgagc gaggcgtaca
     2941 cctatccgca aattgcgcag caaacgggag caagcactgc gacgatcagc agagtaaaaa
     3001 agttccttga gtacggcgcc gacgggtaca aaattgtgct tgatcgtttg aaagaggccg
     3061 gtgaagcgac gaatcccgaa gacaaatctt ccaaggcctg atccttcttc tcttttctaa
     3121 accagcagag ggagctcgaa aaatgctctc ctctgctgat ttacatttgt ttattcttga
     3181 accgacgggc ataggttgtt gctgtgcaac gggcacttcc cacactcggg ggtacgggcg
     3241 tgacacagcc ctcttccgtg ttcaataatg ttgacatgtc ctcccagaaa acgtctctct
     3301 ggaacccatt cttcaagcaa aggctgtatc tcgtcgggag ggagtgattc cgccgcccac
     3361 ttcattctcc ggcagaacct ggcgatatgc gtgtcgaccg gaaaggcaga catccctaag
     3421 tcgaagagca tcacacacgc cacggtcttc gcccctatcc cggggagcga ggaaagatac
     3481 tctcgtactg catcgttgtc catgtctttc aatgccatga gcgaatactc gccgaatctt
     3541 tccttgagtg ccgcgagtat ctggagcatt ctctccgatt ttgtctttgc aagaccagcc
     3601 gggcggattc gatcggcaat agcttcagcc gatgccgatg ccacaagttc ccaagaaggg
     3661 aacgaatttt tcagtgcggc gaagccctta tccctgttgc gatcattcgt gttttgggaa
     3721 agaagtgtga gaatgagccc gtctaagggt tcttcatgtc ccaggcgggg ctcgttcttt
     3781 tcatttcccc acagctcttc cagtatgtcg aaaaccgtgt cgatatcttg cggcctggat
     3841 gggggctctt tcttgctatt cttcttcgga gtcatcgtct cctcctgcct cggagtcttt
     3901 gccttttttg agtttttccg tcttcttcgc ccattttttt agcttgttct ttaccttgcg
     3961 atgaatcgat tctgcggaaa ctccggtaag cgcctcaatc ccttcgtcga tcgtcgagac
     4021 tgcgcggatg aagaatttcc ctttgctgac tgcctcgatc accgtgtcgt cgagcatgag
     4081 atgccgtgtg ttctgcacgg ggatgagtac cccctgctct ccggtgagac cgccgtgttc
     4141 gcagtactcg aagaaacctt cgatcttttc gttgaccccg ccgatgggct ggatgttccc
     4201 gaactggtca accgatccgg ttaccgctat cccctgcttg agcggaacct cggagagggc
     4261 tgagaggagg cagtaaagct ccgtcgagga agcgctgtct ccttcgatcc cgccgtacat
     4321 ctgttcgaat gtcagtttag cggagagtga aagcggcata tcctgcgcgt actttcgccc
     4381 catgtagctg gtcagaatca agagcccctt attgtggata ggtcccgtca tcttgacttc
     4441 tctctcgata ttgacgaccc cgtcctgtcc cataaagacg ttggcggtga ttctggaggg
     4501 gtgtccgaaa cgatagtcca tcaggtcgat gactgaaagg ccgttgatct ggccgactac
     4561 ttttcccgaa gtgtcgatcc tgaccagacc atcctggtag gtgcgtgcta tcttctcttg
     4621 gattaaatcc gaacggaagc gcttttctcg aatagccttc ttcacgtgtt cacgttgaac
     4681 agtatcggca ttttccgctt tagcccaggc tgtggactcg acgatgatct ccgtaatttt
     4741 gttgaactcc gtggagattc gcccttgatc gtcggcgatt ctggccgaac agtctatcac
     4801 ctcggcgact ccggatgcgt cgaaaggaag cccgttttct tttttaaggc acgaagcgat
     4861 gaactgggcc atctgttttt ctgtctcagg cgttcgctcc atgtcggtgt cgaaatccgc
     4921 cttgatcttg aacatcttct gaaactccgg gtcgtaatac tgaagcagcg catgaagaaa
     4981 aggcgttccc accatcacga ccttcatgtt tatcggtatg ggttgagggc gcagggaaga
     5041 gacgggaacg gcgccgtact gctctcccag gttttctatg gtcgcttcgc cggtgcggag
     5101 aatgcgcttc agcgcttccc acgacatgaa attgagcagt actttctcgg cgtcaaggac
     5161 gaggaatcct ccgttggcct tctgaaatgc tccggggaca atttttttga agtcggtata
     5221 aagataccct tggcggcttt cgtattcaac ctttccgccc aaattgtagt atgtagggtt
     5281 tgtctcccaa ataacgggag cgccgttctc gggatcgttg cagacaaaaa cgtttactgt
     5341 gtaccgggta aaatcgatct cggcgttttc atctttgacc gccgccacaa acatgccgaa
     5401 gttgtcaatg atatcttcgg caagggcgtc gatccagctt ccaagaacgt cgttgctctt
     5461 ttgattctca tccttggagg aatccccgtt cgttccaaac ttttctttga tgtcggaaag
     5521 atagggcatg attgcattgc ggcatatttc agcctctagt ttgacaatgc gttccttcag
     5581 tcctttttca aggtcgcgga tctttcggag cgtttcaagc gtcttttggg aaacctcctc
     5641 cgatttcttc tggagacttt tctgttcctc ctcggagagc gcctcaaatt cttcctgctg
     5701 aatctcccgc tttgtttttt ctccgctctc cgtttcttct tccgtcaagg ggatattaac
     5761 aaaaccctgc ggggtcctct tcaaggaaaa accgttttca gcagcccagg ccttgacttc
     5821 atccatgagt tgattgactt cttcctgaaa ctctttgacg agctgtgctt tggcgtcttc
     5881 gtactgactt ttttcgaacg ccttacttat cgatgtcttc agctcttcga ccatttcgtc
     5941 gaacgccgta cccatttctt tccctttgcc tgccgggagg ttgatagcaa gtggctgccc
     6001 cgggtcctca aagttatata catacaccca gtcgtcggga ggagtgcgtt tgcgagctgc
     6061 tgtgcggagg cgttcaagag catacgttgt tctaccgctg ccgggattcc cgagcacgac
     6121 gatgttgtat cccttgctct caacttcgag gccgaaggag atcgaggata cagcccgttt
     6181 ctgaccaagg aactcctcaa gaggtgcaat ttcgttcgtc gttgaaatgc cgagcgaggc
     6241 ggcatcggtt tttttccgaa gctgctcaat tgtgagcgat ttgttttgag cgctccctgc
     6301 tgttattttt gatcccttgc gggaggggtt cgtcgtggct tttgtcatag atgctacctc
     6361 cagagatgtc aatttcattg tttgtatgca ttcacgagag aacagatctg tttcgttttc
     6421 gtgagaaggg caagaaactt ctcagcaatc gatccccttg cgggcaagaa tacctttttg
     6481 ataagggtgc cggatttcaa tcatttctgt aatgagatcg gctcggtcaa tgagttcctg
     6541 cggaggattt ctccccgtaa aaacgagctc cacatgaggc ggtttacgtc caataagagc
     6601 tagcacccgg tctagcggga caagcccgaa cgaaagcgcg acattcagtt cgtcaagaac
     6661 cacgaggtca tactcccccg aaaacaccgc ttcggcgccg atggaaaggc cgcgctccgc
     6721 ctcgctgtaa tccaaaggat ctatcttgtc cggatacacg taatctgctc tccccgtctg
     6781 gaagagcgtc actccgggga gaaactgaag cccttttgct tcgctgtacg gccagccttt
     6841 catgaactgg acgacagcgg atttccctcc gcatccagcc attcgaagca cgataccgag
     6901 cgcgcttgtg gtcttccctt tgccgtgtcc catatacacg tgaaccaaac cgtattcaag
     6961 tatcggcatt gtatctcccc cgtccatcta tgcatttccc ctccatatgc attacaatgc
     7021 cttcagaacg gaggtttgtc aatgcttttt caaacgcgaa ttcttgtttc aggtatagtc
     7081 cagggcgtag ggtttcgccc cttttgtgtt cgtcttgcga attccgagag tatttcaggg
     7141 tatgcgctga atacctcgca cggcgtcgag atggaactgc atggcgagga agagaatctt
     7201 gacagcttcc tcttcagaat cgttcgggaa aaccctccgg cgtccttcat cagctccgtt
     7261 gcaatccttg acgagaaacg gaagtcttcg agtccgcccg ctcgttttga gataatggag
     7321 agccgaaaag aaacactgca gaccgccctg attccggccg atcttgctgt gtgcgcacag
     7381 tgtcttgccg agatgaag
//
LOCUS       DAOPKU010000083         7398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10668_length_7398_cov_4.922784, whole genome shotgun sequence.
ACCESSION   DAOPKU010000083 DAOPKU010000000
VERSION     DAOPKU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10668_length_7398_cov_4.922784"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PK849_03840"
     CDS             complement(<1..1041)
                     /locus_tag="PK849_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPE65288.1"
                     /translation="MKSKLNRNGVNGKRIWKRLVTAFVVCSVIGAAVFMLWPKPLEVE
                     TGLAERRPFTASVVEEGKTRIRHRYTVSSPVNGFLNRTALRPGDVVRAGETVLAVMEA
                     ESSGLLTPRLRAEAEARLKTAEAARALRKSELERAKVSLDLAKKQFERLDRLLPSGGI
                     SRQEWDIAESKMQVAIREVKTAEFALRMAEFEVQQVRSSLIQGEGGGASESVRILSPV
                     DGYVLSVFEESARTVPAGTPLMEVGDPDDIEGEVDLFSVDAAVVAQGAAALIEGWGGE
                     APLRGRVALIERSGFTKVSALGVEEQRVKVRIDFLDPVPPGYRLGDRFRIQVRIILRQ
                     NDDVLQIPLGALF"
     gene            complement(1062..3425)
                     /locus_tag="PK849_03845"
     CDS             complement(1062..3425)
                     /locus_tag="PK849_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPE65289.1"
                     /translation="MRMLDLKLLRDIRGMKGQMTAIALVMACGLTLMIMSRGLLLSLE
                     TTKNSYYTESRFADLFCDLKRAPLFLEPALAMIDGVAQVETSVRGAIILDIPGMKEPA
                     DGFVHSLPEGRPRRLNRIHLRAGRLPEAGNRDEILVSDSFAKAHGFRPGDTIDATVYG
                     VRRSFRICGVAISPEYVYEIRPGGMMPDPERFGVFWMSERELSRALGLEGAFNSVAFR
                     LAPGADAGMVKADIDRLLEPYGGLSAYDRERHVSAKLMEDEVAQLRVFSVAFPVVFLS
                     IAAFMVSAALTRLVKLQREQIAQMKAFGYSGFSVASHYVKFAFVPVVAATLLSGTLGM
                     WAAHAFIGLYQRFFNFPALIFRPDWSALLVALLASGLVSFLGVLGAVRQAASLPPAEA
                     MRPEAPADYSPSFLEKAGLHRLVQPSFRIALRNLERKPWQAFFTALGLALAAAIPVLP
                     GAMADGVDYLVDFQWNMAMRQDAVVTFIEPGGGEALLSLSRFPGVLYAEPFRAVPVRL
                     FNGHRERTAAITGMPAAPRLNRLLDSQERVVPVPGSGLLLSEKLAEVLDLSPGDEIRM
                     EVREGRRPVLATFLSGTITDFTGVGAYMEIEGLRRLLGEGTTASGAYLQVDSLYWDEF
                     WRRVKETPRIGSVTTTAGARESFSKTTEEMMGVSQMLYLVFSLIVSFGVIYNGARIAL
                     SERSRSLATLRVLGFTRREVTTILLSELGLLTLLSIGPGLLLGTWMAKSILESVDTET
                     MRFPLILGARTYVVAVLTVFVSTVFSFVVVGRRIAGLDLLAVLKSAE"
     gene            complement(3432..4157)
                     /locus_tag="PK849_03850"
     CDS             complement(3432..4157)
                     /locus_tag="PK849_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65290.1"
                     /translation="MAMNAEYTPLFRARGITKIYHPGTETEVRALDGVDLDLYEGELA
                     VLLGASGSGKSTLLNILGGLDVPTDGTLSYRDEELTGAGERELTLFRRNVAGFVFQFY
                     NLIPSLTARENVALITEIAPDPMEPEEALGLVGLSDRMDHFPSQLSGGQQQRVAIARA
                     IAKRPEVLLCDEPTGALDVHTGITVLEAIERVNRELGTLAVIITHNAAIAAMADRVLS
                     LSAGRILSIHSNEKRAPVNTLSW"
     gene            4467..5312
                     /locus_tag="PK849_03855"
     CDS             4467..5312
                     /locus_tag="PK849_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE65291.1"
                     /translation="MDAGRFTALIDSFLRSSGWNTVAAGRAINEEMTGLRIFDAPLTG
                     VASADDPAFRDLRRESVVGPHFLLPEEWLPGARAVVSFFLPFSENVRRANRADRDEPA
                     PEWLHGRIEGQECIDRLSAFLVERLLEEGFDAVAPSVDERFRSCTTTNTVFLDGDGAP
                     LPVSFNSNWSERHVAYVCGLGSFGLSKGLITPKGVAGRFGSVVTSAPIPPDNVVASAV
                     YENCTMCGACVRNCPVGAITIEKGKDHVRCADFLTLTKEKYRPRYGCGKCQTGVPCEC
                     GIPPR"
     gene            complement(5329..5616)
                     /locus_tag="PK849_03860"
     CDS             complement(5329..5616)
                     /locus_tag="PK849_03860"
                     /inference="COORDINATES: protein motif:HMM:NF023159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPE65292.1"
                     /translation="MNPEKVNRRTVRRLTDLPNVGPATERDLRLLGVEKPEDLAGRCP
                     FEMYDELCEKTGVRQDPCVIDVFMSVTDFMNGSAPRAWWTFTEERKRKTAR"
     gene            6096..>7398
                     /locus_tag="PK849_03865"
     CDS             6096..>7398
                     /locus_tag="PK849_03865"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPE65293.1"
                     /translation="MNDAHVRIFDTTLRDGEQAAGINLNKEEKLQIAMQLAKMKTDII
                     EAGFPAASPGDFEAVKLIAQQVDGPIIAGLARTRREDILAAAEAVKWSKKPRIHTFIA
                     TSPIHMEFKLKMTPEQVLAEVRQAVTYARSLVEDVEFSAEDASRSDPAFLGEVFRTAV
                     ECGATTLNIPDTVGYSTPDEFGEFVREVIEATGNPVGVIWSVHCHNDLGLAVANSLAA
                     VRAGARQVECTVNGLGERAGNASMEEIVMGLRTRKDRFGCQTAIDTTRLCAVSSQVSR
                     MTGFPVPPNKAIVGANAFAHEAGIHQHGVLCNKATYEIMNPEDVGAPGSRLVLGKHSG
                     KHAFKQRVEEMGFTLSDEKLKEAMALFKTLCDRKEIVTADDLEAMINNEILSIDESRR
                     ITLKNFLVQVGSGHGSATVTIEDEGVEKTDAAAGNGPVDAAY"
BASE COUNT         1488 a         2369 c         2247 g         1294 t
ORIGIN      
        1 gaaaagcgcg cccagcggta tttgcagcac atcgtcgttc tggcgcagaa tgatgcgcac
       61 ctgaatccgg aaccggtccc cgagacgata ccccggcggc acgggatcga ggaagtcgat
      121 ccggaccttc acccgctgct cctcaacccc cagcgccgat accttggtaa aaccgctccg
      181 ttcgatgagc gcgacgcgcc cgcgcagcgg cgcttctcca ccccatcctt cgataagcgc
      241 cgcagcaccc tgcgccacga cggccgcgtc cacggagaag aggtcgacct cgccctcgat
      301 atcgtcggga tcccccacct ccatgagcgg cgtcccggcg ggaacggtcc gggcgctctc
      361 ctcgaagacg ctcagcacgt agccgtccac aggggagagg atgcgaacgg actcggacgc
      421 cccgcccccc tccccctgga tgagcgagga tctgacttgc tgcacctcga actccgccat
      481 acggagcgca aattccgccg tcttcacctc tcggatggcg acctgcattt tgctctcggc
      541 gatatcccac tcctgcctcg aaattccgcc cgaaggaagc agtctgtcca ggcgctcgaa
      601 ctgcttcttc gcgagatcga ggctcacctt cgcccgttca agctccgact ttcgcaacgc
      661 gcgcgccgct tcggccgtct tcagccgtgc ttcggcttcc gcacgaagcc gaggcgtcag
      721 caggcccgag gattccgcct ccataaccgc aagaacggtc tcgcccgcac ggacgacgtc
      781 tccggggcgc agcgccgtac ggttcagaaa gccgttcaca ggggaggaga ccgtgtaccg
      841 gtgtcgtatc cgcgtctttc cttcctccac gacgctcgcg gtgaagggcc tgcgctccgc
      901 aagccccgtc tccacttcca gaggtttcgg ccacagcatg aagacggccg cgccgatgac
      961 cgagcacact acgaaagctg tcacgagcct tttccagatt ctttttccgt tgacgccgtt
     1021 tctgttcagt ttcgacttca ccaactccac cccgtttcgc gctattcagc ggatttcagc
     1081 accgcgagca gatcgagccc ggcgatccgt cgtcccacca cgacgaaaga aaaaaccgtg
     1141 gacacaaaca cggtcaacac tgcgacgaca tacgtcctcg ctccgagtat cagagggaag
     1201 cgcatggtct cggtgtccac cgactctaga atggatttcg ccatccacgt ccccagcagg
     1261 agcccgggac caatcgaaag aagcgtgaga agccccagtt ccgaaaggag aatggtcgtc
     1321 acttcgcggc gggtgaaccc gagcacgcgc agcgtcgcca gcgagcggga gcgctccgaa
     1381 agagcgatgc gcgccccgtt gtagatcact ccgaaggaga cgatgaggga aaagacaaga
     1441 tagagcatct gcgacacgcc catcatctcc tcggtggtct tgctgaaact ctccctggcg
     1501 cctgcggtcg tggtcaccga accgatgcgg ggagtttctt tcaccctccg ccaaaattca
     1561 tcccagtaca gcgagtccac ctgcaggtaa gcgccgcttg ctgtcgtacc ctctccaaga
     1621 agcctgcgca gtccttcgat ctccatatag gcgcccaccc cggtaaagtc ggtgatagtt
     1681 ccggagagga aggttgcgag cacgggacgg cgcccttctc gcacttccat tcggatctcg
     1741 tcgccggggg agaggtccag cacttcggcg agtttctcgg agagcagcag cccggaaccg
     1801 ggtacgggga caacccgttc ctgagagtcc agaaggcggt tgagacgggg cgccgccggc
     1861 attccggtga tcgccgccgt ccgctccctg tgtccgttga acagcctgac aggaaccgcc
     1921 cggaacggct cggcgtagag aacccccgga aagcgcgaca acgagagcag cgcctcaccc
     1981 ccgcccggtt cgataaacgt gacgaccgcg tcctgacgca tcgccatgtt ccattgaaag
     2041 tccacaaggt aatcgacgcc gtcggccatc gctcccggaa gcacgggaat ggccgcggcg
     2101 agcgccagcc cgagagcggt gaaaaaagcc tgccacggtt ttcgttccag attgcgcagc
     2161 gcgatcctga aggaaggttg caccagcctg tggagtccgg ccttctccag aaaggacggg
     2221 gagtaatccg cgggagcctc cggccgcatc gcctccgcgg gagggagcga cgccgcctgc
     2281 ctgaccgcgc ccagcacccc gaggaacgat accaacccgc tcgccagcag cgcgacgagc
     2341 aaagcgctcc agtcgggacg gaagatcaac gcagggaagt tgaaaaagcg ctggtagagc
     2401 ccgatgaagg cgtgggccgc ccacatgccg agcgtgccgc tgagcagcgt tgcggcgacc
     2461 accgggacaa aggcgaactt cacgtagtgc gaggccacgg aaaagccgga gtatccgaac
     2521 gccttcatct gcgctatctg ttcgcgttgc agcttgacga gacgggtcag cgccgcgctc
     2581 accataaacg cggcgatgct gaggaaaacg acgggaaacg ccacagaaaa cactctgagc
     2641 tgggcaacct cgtcctccat cagctttgcc gagacgtgcc gttcacggtc gtacgcagac
     2701 agccctccgt acggctcaag aagcctgtct atgtccgctt tcaccatccc ggcgtccgct
     2761 cccggcgcaa gccggaaggc aacgctgttg aaggcgccct caagccccaa cgcccgcgag
     2821 agctcgcgtt cgctcatcca gaaaaccccg aagcgttccg ggtcgggcat catgcctccc
     2881 ggtctgatct cgtagacata ctcgggggag atggcgacgc cgcagatacg gaagctgcgc
     2941 cgcacgccgt agacggtggc gtcgatcgta tctccggggc ggaagccgtg ggctttggca
     3001 aaggagtcgc tgacgagaat ttcgtccctg tttcccgcct cgggaagacg ccccgcgcgc
     3061 agatgaatgc ggttgagccg ccgggggcgt ccctccggaa gcgagtggac gaagccgtcc
     3121 gcaggctcct tcattccggg aatatcgagg atgatcgctc ccctgacgct cgtttccacc
     3181 tgcgccacgc cgtcgatcat cgcgagggcg ggctcgagga acaggggagc gcgcttgaga
     3241 tcgcagaaga gatccgcgaa gcgcgattcc gtgtagtagc tgttcttcgt cgtttcaagg
     3301 ctgagaagca gtccgcgcga catgatcatc agggtcagac cgcacgccat caccagggcg
     3361 atggcggtca tctgcccctt cattccgcgg atatccctga gcagtttgag atcaagcatc
     3421 ctcattgcac atcaccagga gagcgtgttc accggcgcgc gcttctcgtt cgaatgaatg
     3481 gagagaatac gccccgccga gagagagagc acccggtcgg ccatcgccgc gatcgccgcg
     3541 ttgtgcgtga tgatgacggc gagcgttccc agctctctgt tcacccgctc gatcgcctcc
     3601 agcactgtga tgccggtgtg cacgtcgagc gccccggtcg gctcgtcgca gagaagcacc
     3661 tccggacgct tcgcgatggc gcgcgcgatg gcgacccgct gctgctgccc tccggagagt
     3721 tgcgagggga agtggtccat cctgtccgaa agaccgacga gaccgagcgc ctcttccggc
     3781 tccatgggat cgggcgcgat ctcggtgatg agcgccacgt tctcgcgcgc ggtaaggctc
     3841 ggaatgaggt tgtagaactg gaagacgaac cccgcgacat tcctgcggaa gagcgtgagc
     3901 tcccgctcgc ccgcgccggt gagctcctcg tccctgtacg agagcgtccc atccgtcggg
     3961 acgtccagtc cgccgaggat attgagcagc gtggacttcc cgctccccga ggcgccgagg
     4021 agcacggcca gctccccctc gtagagatcg agatcgacgc cgtcgagggc gcgcacctcc
     4081 gtctccgtcc ccggatggta aatcttcgtg atcccccgcg ctcgaaagag gggggtgtac
     4141 tccgcattca tcgccatcaa cgttcctcct gaaaaacacg cgctgcaccc cctgcacgca
     4201 gtatcggcag cgggaagttc ctgcaacgtt atatcccaat gggctgcatc agtcaaacaa
     4261 tagcggaaga tttccgcaga tttttcatcg gacgctttgc ggaaaaagac tggcatggac
     4321 cgacagatct tggaaaacag ggccttttca cgtagtatag aggctgcaaa cgagggaggg
     4381 aagacgcgga ttcccgcggt gaacgaggcg gtgagcgcgt tgtattaaaa gctccccgcc
     4441 gtgatatgga aaggacggag atctctatgg atgcaggacg gtttactgcg ctgatcgaca
     4501 gttttctgcg gagttccggt tggaacacgg tcgcagccgg gcgcgccatc aacgaagaga
     4561 tgaccggact ccggatcttc gacgcgccgc ttacgggcgt cgcctcggcg gatgaccccg
     4621 ccttccggga tcttcgcagg gagagcgttg tggggccgca cttccttttg ccggaggagt
     4681 ggcttcccgg cgcgcgcgcc gtggtttcgt tctttctccc gttctcggag aacgtgcgcc
     4741 gcgcaaaccg cgccgacagg gacgaaccgg cccccgagtg gctgcatggc aggatcgaag
     4801 ggcaggagtg catcgaccgt ctttccgcgt tccttgtcga gcgtcttctg gaagaaggat
     4861 tcgacgcggt cgctccgtcc gtcgacgaac gcttccgctc ctgcacaacg acgaacacgg
     4921 tgtttctgga cggcgacggc gctcctctgc cggtatcgtt caacagcaac tggtcggagc
     4981 ggcatgtcgc ctatgtctgc ggactggggt cgttcgggct ctccaagggg ctgatcacgc
     5041 cgaaaggcgt cgcggggaga ttcggcagcg tggtcacgag cgcgccgata cctccggaca
     5101 acgtggtcgc ctcggccgtg tatgaaaact gcaccatgtg cggcgcgtgc gtccgaaact
     5161 gtccggtcgg cgccatcacc atcgaaaagg ggaaggatca cgtccgctgc gcggattttt
     5221 taactctgac gaaagaaaaa taccgcccgc gatacgggtg cgggaagtgc cagaccggcg
     5281 tcccgtgcga gtgcgggata ccgccgaggt aatcccgcgc gggccgcgtc agcgggccgt
     5341 tttccgcttc cgctcctccg tgaacgtcca ccacgcgcgc ggagcggaac cgttcatgaa
     5401 gtcggtcacc gacataaaga cgtcgatcac gcaggggtcc tgccggactc ccgtcttctc
     5461 gcagagctcg tcgtacatct cgaacggaca gcgaccggca aggtcttccg gcttctcaac
     5521 gccgagaaga cggagatctc tttccgtggc cggtccgacg ttgggcagat cggtcagccg
     5581 ccggaccgtc cgacgattca ctttttcggg gttcatcgca cgcacctctc ctcgaaaggg
     5641 cgccggagcg ccctttgcga agcattctac cacgccggga ttttccacgc gacccggtca
     5701 aagttatagc cttttatcag aataaacgcc ttttgttttt cttttgtttg agtattgaca
     5761 ttccaggggt actccctcaa aatgtaccaa tcttaccgag ccatgaggag aggagggaat
     5821 cagttatgtt aagtagacga ccggcgaaca cgagcgaagc gagtaagaag ctgtgtcgat
     5881 cacttatagc gcatgcaact ggcgctaaag aagtacgttc acgcatcatg aacgcttcct
     5941 ccctgtgcct cctcctcggt tgccggttcc cgcctggaac gccggcagcc cggtaagctg
     6001 cgtaacggca ttccaaggcc ggaacctctt ttttagaggt tccggccttt ttttgtactt
     6061 ttatttcacg gatgattttg aaaggagaga gaattatgaa cgacgcacac gtacggattt
     6121 tcgataccac cctgagagac ggagagcagg cggcggggat caacctgaac aaggaggaga
     6181 aactccagat cgctatgcag ctggcgaaaa tgaagacgga catcatcgaa gcggggttcc
     6241 ccgccgcttc ccccggcgac ttcgaggcgg tcaagctcat cgcgcagcag gtggacggcc
     6301 cgattatcgc cggacttgcc cggacacgac gggaagatat cctcgcagcc gccgaggcgg
     6361 tcaagtggtc gaagaaacct cgcatccaca cgttcatcgc cacaagcccc atccacatgg
     6421 agttcaaact caagatgacc ccggagcagg tgctcgccga ggtgcggcag gccgtgacgt
     6481 acgcgcgcag tctcgtcgag gacgtcgagt tctccgcgga ggacgcaagc cgctcggacc
     6541 ccgcttttct gggagaggtc ttccggacgg cggtcgagtg cggcgcgacg acgctcaaca
     6601 tccccgacac cgtaggctac tcgaccccgg acgagttcgg agagttcgtc cgcgaggtca
     6661 tcgaggccac tgggaaccct gttggcgtga tctggtcggt ccactgccac aacgacctcg
     6721 gactcgcggt ggcgaactcg ctcgcggcgg tcagagccgg agccaggcag gtggagtgca
     6781 ctgtcaacgg tctcggagag cgggccggca acgcctccat ggaggagatc gtcatggggc
     6841 tgcgcacgcg gaaggaccgc ttcggctgcc agaccgcgat cgacacgacg cgcctctgcg
     6901 ccgtgagctc tcaggtctcc cgtatgacgg gctttccggt gccgccgaac aaggccatcg
     6961 tcggggcgaa cgccttcgcg cacgaagcgg gaatccatca gcacggcgtg ctgtgcaaca
     7021 aggcaaccta tgagataatg aacccggaag acgtcggcgc ccccggaagc cgtctcgtac
     7081 tgggcaagca ctccggaaag cacgccttca agcagcgcgt ggaagagatg ggcttcaccc
     7141 tctccgacga gaagctgaaa gaggccatgg cgctcttcaa gacgctctgc gaccggaagg
     7201 aaatcgtcac agccgacgac cttgaggcca tgatcaacaa cgagatcctc tcgatcgacg
     7261 agagccgccg catcacgctg aagaacttcc tcgtgcaggt cggcagcggg cacggcagcg
     7321 cgaccgtcac tatagaggac gagggcgttg aaaaaaccga cgcggctgcg ggcaacggcc
     7381 cggtggacgc ggcttaca
//
LOCUS       DAOPKU010000084         7365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10722_length_7365_cov_4.576881, whole genome shotgun sequence.
ACCESSION   DAOPKU010000084 DAOPKU010000000
VERSION     DAOPKU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10722_length_7365_cov_4.576881"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PK849_03870"
     CDS             complement(<1..502)
                     /locus_tag="PK849_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPE65294.1"
                     /translation="MVNELKRKLARGEVALGTFITLNCPDLVEIAGLAGFDFCIIDTE
                     HGPGDAESIQHMIRAAELRGITPVVRVTNPEATTILRILDVGAAGIQAPQVNSRKTAE
                     DVVRFAKYFPKGERGACLTRSSRYGFVEGVAQYFEEANRETMVILHCENKQGLECLDD
                     IASVEGV"
     gene            complement(609..1532)
                     /locus_tag="PK849_03875"
     CDS             complement(609..1532)
                     /locus_tag="PK849_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HPE65295.1"
                     /translation="MKKMTTHLRALIARPGALVVPGVYDALSARICEMVGFEAVFHSG
                     YGTSAVHLGQPDIGLLDMTEMSSQVRSIARAVRIPVLGDSDHGFGNAVNAYRTVQEYL
                     WAGAAGLFMEDQVSPKRCGHMEGKQVISKEEMMGKLRAALDARNELDPDFVVMYRTDA
                     IHVTGFEDALERAKAAVDVGVDMVFVEALETREQMERVVAEVKVPLMLNLIEGGKTPL
                     VPVKEAEAMGFKLIVPALSTLYSAARGMYDVMKRIHAEGVSDGYLDKLFTFKEFGELV
                     RLDEVRAKEEKYLPPSVMQDKYRGKKHIVEK"
     gene            complement(1529..2428)
                     /locus_tag="PK849_03880"
     CDS             complement(1529..2428)
                     /locus_tag="PK849_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HPE65296.1"
                     /translation="MEKRGAQLRKLLARREILVAPGAYDGLSARLVESAGFEAVYVTG
                     YGLSASVLGKPDTGLLSLTEMASRVSAIAETVSVPVIADGDTGYGNAVNVARTVREYE
                     KAGAACIQLEDQVAPKKCGHMLGREVIPAAEMAGKIRAACDARRDDDFLIMARTDART
                     THGIDEAIERGALYEEAGADILFIESPESVEEMRRITSSFGVPVIANMVEHGRTPFLK
                     TSELEEIGYDIVIFPVGPLYAAAKAVGAVLETLKRAGTTADCIKDMIPFAEFNALMGL
                     DGIRDMEKKYATGRDGRTEEENA"
     gene            complement(2442..3722)
                     /locus_tag="PK849_03885"
     CDS             complement(2442..3722)
                     /locus_tag="PK849_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE65297.1"
                     /translation="MLSFSFVVVLLIGILFMGLPVAFSLGGTSLVLIWLFELPMKVVG
                     ATIFTSLEGFVLLAIPLFVLMSQILLDGRIGDDLFDVMNAWVRHLPGGLAIATILACA
                     FFAAITGSGAATAATIGMVAYPAMTERGYDKRFTLGLLAAGGTLGILIPPSIPLILYG
                     AITEESVGKLFIAGVVPGLVLTALFIVYAVYKSKRGGFTPMERTSWSERLRVTRKNIW
                     GIFLPFLIIGGIYSGAFTPTEAAAVGLVYSLFITLFVYRTLSFRDIPAICLRSVTTSC
                     MVAIIIAGAILFGRVMTLLRIPQILTELVISNNFSVLMFIIAMNVLMMILGCILETVS
                     IILLTMPLVTPILHALGIDPIWYGIVVTVNMTLALITPPVGMNLYVINGLRSDIRMGE
                     VIQGVTPFIVIMALFLIGVIAFPAMSTWLPSIMQ"
     gene            complement(3716..4240)
                     /locus_tag="PK849_03890"
     CDS             complement(3716..4240)
                     /locus_tag="PK849_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE65298.1"
                     /translation="MIRHIRSAVEKGNLILGYVSGLGILLMGLILFYEVVMRYFFKAP
                     TIWAQETAVYIFMWTMLAGGAYTLQKGKHVRIDLVIEHLSIRTQKILEMITSFAGMFF
                     CAVVTWQAYEMIAASIGYEKVSATPLRVPMWIPQMALLLGFALLTFQFAIIIADRYAE
                     VFGKGAAGEEKKTC"
     gene            complement(4346..5338)
                     /gene="dctP"
                     /locus_tag="PK849_03895"
     CDS             complement(4346..5338)
                     /gene="dctP"
                     /locus_tag="PK849_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE65299.1"
                     /translation="MFKKSFATTAFLVLALSLLCTAAFAKVEIKLSNQLPPSHHISKG
                     LVVFSEKVAEYSGGDVTVRIFDSAQLFKDTEIVEALQDGLVEAGLVPVNKWSGMIPAA
                     DIFEMPFVFKDFESPKKFLNSGADKLLDGEFQKKGVKNLFWVDYGYIQFFNNKRPLKS
                     PEDFKGLTMRTFSSGDAETLRALGAAPTVMSSSEMYMALQRGTVEGATTGMPAAVSRK
                     IHEVQKYMTMANYTTAQFVVQANLGWWEKLSQAEKDVILKAGLDAEAWIRQAIEASEN
                     EAEKVIRDAGVEIHVLSAEERPLFIEATDPVRKAFAEKTGEIGKTLIDMVMAID"
     gene            5619..>7365
                     /locus_tag="PK849_03900"
     CDS             5619..>7365
                     /locus_tag="PK849_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPE65300.1"
                     /translation="MFCMMSKNDWFPDNEWFPGDDSSSPKRPVFRLPKVNKGLLLLGG
                     AVLLVMVLFPALAEFYTDWLWYDTEGYGSVFLTRILARLPLFAAGFLLFASAIWVNLA
                     FVRRNLLALHPEFTPLLASKAAGWAIGAVALFLGFSNGVAASTEWAMVLRFFNATPFG
                     TADPIFGNDTGFYVFALPFWTFLHTRLLNILFLCVVTAAAAYGAIILPRNRDFSMITI
                     PQSVRTHLTLLASAGAFLWGIGYLLDRYGILFSPRGVTFGAGYTDVHAELFALNVLAV
                     ISILIAAALLVSLFRKMWKLSLGMVGLLVVSSVLLRGVYPAIVQKYFVEPNEFERERR
                     FIDYNIAATLHAYNLHNVELKTITPDDAITWRDIEENRDTTDNIRLWDHGPLLRSYKQ
                     LQEIRLYYDFANVDIDRYKFPSVSGTGSSEFRQVMLAARELDIRGLQNPTWINTRLEF
                     THGYGIVMNPVNEVAGSGLPRLWVRDLPPRSDVELPITHPQIYYGEKPSSYIFVRTTV
                     QEFDYPMGDSNVRTVYEGKGGVPMGSFFRRILFALKFADMKILLSDVFTEESRVKFHQ
                     NIQERLMRVAPFLYFD"
BASE COUNT         1440 a         2372 c         2042 g         1511 t
ORIGIN      
        1 ccaccccctc cacggaagcg atgtcgtcga gacactccag cccctgcttg ttctcgcagt
       61 ggaggatcac catcgtctcg cggttggcct cctcgaagta ctgcgccacc ccctccacaa
      121 acccgtaccg ggacgaccgc gtaagacatg cgcctcgctc ccccttcggg aagtacttcg
      181 cgaagcggac cacgtcctcc gccgtcttcc gcgaattcac ctgcggggcc tgaattccgg
      241 cggcgcccac gtcgaggatg cggagaatcg tggtggcctc cgggttcgtc accctgacga
      301 ccggcgtgat cccccgaagt tcggcggcgc ggatcatgtg ctggatggac tccgcgtcgc
      361 cggggccgtg ctcggtgtcg atgatgcaga agtcgaaccc cgcgagcccc gcgatctcga
      421 ccagatcggg gcagttcagc gtgatgaacg tgccgagagc gacttcgccc cgggcaagct
      481 ttcttttcag ttcattgacc attcaagtat ccctccgtcg actgcattct gcgtttccga
      541 tacatattat accctcgaaa aaggggcgga ggttttcccc cgcccctgcg tttcgacaaa
      601 cttcccggtc acttttcgac gatgtgcttc ttccccctgt acttgtcctg catcacgctc
      661 ggggggaggt acttctcctc cttcgcgcgg acctcgtcga ggcggacaag ctcgccgaac
      721 tccttgaacg tgaagagctt gtcgagatag ccgtcggaga ctccctcggc gtgaatcctc
      781 ttcatgacgt cgtacatgcc tcgggctgcg gagtagagcg tcgacagcgc gggtacgatc
      841 agcttgaagc ccatggcctc ggcctccttc accgggacga gcggcgtctt gcccccctcg
      901 atgaggttga gcatcagcgg aaccttgacc tccgcgacga cccgctccat ctgctccctg
      961 gtctccaagg cctcgacaaa gaccatgtcc acgccgacat ccaccgccgc tttcgctctt
     1021 tcaagcgcat cttcgaaccc cgtcacgtgg atggcgtcag tccggtacat cacgacgaag
     1081 tcgggatcca gctcatttct cgcgtcgagc gccgcccgca gcttgcccat catctcctct
     1141 ttggagatga cctgcttccc ctccatatgc ccgcaccgct tcggcgacac ctgatcctcc
     1201 atgaagaggc cggccgcccc cgcccagagg tactcctgca ccgtccggta cgcgttgacc
     1261 gcgttgccga acccgtggtc cgagtcgccc aaaacgggaa tccgcacggc tctggcgatc
     1321 gagcgaacct gggaggacat ctccgtcatg tcgagcagcc cgatgtccgg ctgtccgaga
     1381 tggactgccg acgtgccgta tcccgagtga aagaccgcct cgaagccgac catctcgcag
     1441 atgcgcgcgc tcagcgcgtc gtacaccccc ggaacgacaa gcgctccggg acgcgcgatc
     1501 agtgcgcgaa gatgcgttgt catcttcttc atgcgttctc ctcctccgtc cggccatcgc
     1561 ggccagtggc gtattttttc tccatatcgc gaataccgtc cagccccatc aatgcgttga
     1621 actccgcgaa gggaatcatg tccttgatac aatccgccgt ggtcccggcg cgcttcagcg
     1681 tctccagcac cgcgccgacg gctttcgccg cggcgtagag cgggccgacg gggaagatga
     1741 cgatgtcgta cccgatctcc tccagctcgg aagtcttcag aaaaggagtc cgtccgtgct
     1801 ccaccatgtt ggcgatcacc ggaacgccga agctcgacgt gatgcgccgc atctcctcca
     1861 ccgactccgg cgattcgatg aagaggatat cggctcccgc ttcctcgtag agcgctcccc
     1921 tctcgatagc ctcgtcaatg ccgtgcgtcg tccgcgcgtc cgtccgcgcc atgatgagaa
     1981 agtcgtcgtc cctcctggcg tcgcaggccg ccctgatctt gccggccatc tccgccgcag
     2041 ggatcacttc gcgtcccagc atgtgcccgc acttcttcgg agcaacctga tcctccagct
     2101 gaatgcaggc cgccccggcc ttctcgtact cgcggacagt ccgcgccacg ttgaccgcgt
     2161 tgccgtaccc cgtgtcgccg tcggcgatga ccggaacgga aaccgtctcc gcgatcgccg
     2221 acactctcga ggccatctcc gtcagcgaga gcagtccggt atcgggcttt cccagcacgc
     2281 ttgcagagag accgtacccc gtcacgtaga cggcctcgaa cccggcggac tcgacgagcc
     2341 gggcggaaag accgtcgtaa gctcccggcg cgacgagaat ctcccgccgc gccaggagct
     2401 tgcgtagctg tgctcctctt ttttccatga tcatccgtcc gttactgcat gatcgacggc
     2461 agccacgtgc tcatcgccgg gaaggcgatg actccgatca ggaagagcgc catgatgacg
     2521 atgaacgggg tgacgccctg gatgacctct cccatccgta tatccgacct cagtccgttg
     2581 atgacataga ggttcatacc cactggaggc gtgatcagcg ccaatgtcat gttcaccgtg
     2641 accacgatac cgtaccagat cgggtcgata ccgagcgcgt ggagtatcgg ggtcaccaat
     2701 ggcatggtaa gcaggatgat ggagaccgtc tccagtatgc agccgaggat catcatcagc
     2761 acgttcatcg cgatgatgaa catcagcacg gagaagttgt tgctgatgac gagttcggtg
     2821 aggatctgcg gaatccgcag cagcgtcatc acgcgtccga agagaatggc gccggcgatg
     2881 atgatcgcca ccatgcacga cgtagtaacg gagcggaggc agatcgccgg gatgtcgcgg
     2941 aaactcagcg tcctgtagac gaagagcgtg atgaaaaggc tgtagacaag ccccacggct
     3001 gcggcctcgg tcggagtgaa tgcgccggaa taaatacctc cgataatgag gaaggggagg
     3061 aagatacccc agatgttctt ccgtgtaacc ctgaggcgct cgctccagga ggttcgctcc
     3121 atgggggtaa aaccgccccg cttgctcttg tagactgcgt acacgataaa gagcgccgtc
     3181 aggacgagac cggggacaac ccccgcgatg aagagctttc cgacggactc ctcggtaatc
     3241 gcgccgtaga ggataagggg aatgctcggc ggaatgagga tgccgagcgt gccgcccgcg
     3301 gccagcagac cgagagtgaa tcgcttgtcg tatccgcgct ccgtcatcgc cgggtaggcc
     3361 accatcccga tcgtcgccgc ggttgccgcg ccggagccgg tgatggcggc gaagaaagcg
     3421 cacgcaagaa tcgtcgcgat ggcaagcccg ccgggaaggt gccgcaccca cgcattcatg
     3481 acatcgaaaa ggtcgtcgcc gatccgtccg tcgagcagta tctggctcat cagcacgaag
     3541 agcggaatgg caagcagcac aaagccttcg agcgatgtga atatggtcgc gcccacgacc
     3601 ttcatgggaa gctcgaaaag ccagatcagc acaagcgacg ttccaccgag agaaaaggcc
     3661 accggaagtc ccatgaagag aattccgatg agcagcacga cgacgaagga aaagctcagc
     3721 atgttttttt ctcctctccg gcggcgccct tgccgaatac ttccgcgtag cggtcggcga
     3781 taatgatcgc aaactggaat gtcaacagag cgaaaccgag cagcagcgcc atctgcggaa
     3841 tccacatcgg cacccgcagc ggcgtcgccg atactttttc ataccctatg gacgcggcga
     3901 tcatctcgta cgcctgccac gtgaccaccg cgcagaagaa cattccggcg aagctggtga
     3961 tcatttcgag gatcttctgc gtccgtatcg agaggtgctc gataacgagg tcaatgcgca
     4021 cgtgcttccc cttctggagc gtgtaggctc ctccggcgag catcgtccac atgaagatgt
     4081 acacagcggt ctcctgggcc cagatggtgg gcgccttgaa gaaatagcgc ataacaacct
     4141 cgtaaaacag aatcagcccc ataagaagaa tcccaaggcc gctgacgtag cccaggatca
     4201 ggtttccttt ctccaccgca ctgcgaatat gtcgaatcat ctacgcatgc ccctttattc
     4261 ataatttata gtaaagagcg ggaaagagag tccctttccc gctctttcga gacaactccc
     4321 gtgaggactc tttctcccga aaaacctagt cgatcgccat aaccatgtcg atcagggttt
     4381 tgcctatctc tcccgttttt tcggcgaagg ccttgcgcac aggatccgtg gcctcgatga
     4441 agagcggcct ctcctcggcg ctcagtacgt ggatctccac tccggcgtcg cggatcactt
     4501 tttcggcttc gttctcggac gcctcgatgg cctggcgaat ccacgcctcg gcgtcaagac
     4561 cggccttcag gatcacatcc ttctcggcct gggagagttt ctcccaccag ccgaggttcg
     4621 cctgcaccac gaactgcgcc gtagtgtagt tcgccatcgt catgtacttc tgcacttcgt
     4681 ggatcttacg ggagacggcg gccggcatac cggtcgttgc gccttctacg gtgcctctct
     4741 gcagcgccat atacatctcg gacgaactca tgacggtggg cgcggcgccc agcgcgcgca
     4801 gcgtttccgc gtcgccgctg ctgaaggttc tcattgtaag gcccttgaag tcctcggggg
     4861 acttcagggg acgcttattg ttgaagaact gaatgtaccc gtagtcaacc cagaagaggt
     4921 ttttgacgcc cttcttctgg aactcgccgt caaggagctt gtccgcgcca gagttaagga
     4981 atttcttggg ggactcgaaa tccttgaaga cgaagggcat ctcgaagatg tccgccgcgg
     5041 ggatcatgcc ggaccacttg ttgaccggca ccaaacccgc ttcaacgaga ccgtcctgaa
     5101 gcgcctccac tatttccgta tccttgaaga gctgggccga gtcgaagatc cggaccgtaa
     5161 cgtctcctcc ggaatactcc gccacctttt cggaaaaaac gacgagcccc ttcgagatat
     5221 gatgcgaggg ggggagctgg ttggaaagct tgatctccac cttggcgaac gcggcggtgc
     5281 agagcagaga gagcgcgaga accagaaatg ccgttgttgc aaatgacttt ttgaacatac
     5341 tttgatttcc tcctttaccg aaatgtgttg aaactttcct gaacaaataa cgtgccccgt
     5401 gttgatacgc ggattactct tgttaccata cgcatcggcg ctctgtcaag ataaatcgct
     5461 ccggagcggt attttcaaat tactccccca ccttcaattc caccctttga aataaaagaa
     5521 ctcggcgcgg catatttctc tttcgacatc aacggatggc atatcctcca gcgctgcgct
     5581 gtttttcact ctttgacaaa ggtagaaagt ccgttacaat gttttgtatg atgagtaaaa
     5641 acgactggtt tcccgacaac gagtggttcc cgggcgacga ttcatcgtct cccaagcgtc
     5701 cggtcttccg gcttccgaag gtgaacaaag gtctcctgct gcttggaggc gccgttctcc
     5761 ttgtaatggt gctgttcccc gccctggcgg aattttatac cgactggctg tggtacgaca
     5821 ccgaagggta cgggtccgtc tttctgaccc gcatcctcgc caggcttccc ctgttcgctg
     5881 cgggcttcct cctcttcgcc tcggccatct gggtcaacct ggccttcgtc cggcggaatc
     5941 tgctggctct ccatcccgaa ttcacaccgc ttctcgcgtc gaaagcggcc gggtgggcca
     6001 tcggagccgt cgcgctcttc ctgggctttt ccaacggcgt cgccgcctcc acggagtggg
     6061 cgatggtgct gcgcttcttc aacgcaacgc ctttcggcac ggcggatccc atcttcggca
     6121 acgacacggg cttttacgtc ttcgccctgc ccttctggac gtttcttcac acgcggctgc
     6181 tgaatatcct cttcctctgc gtagtcaccg ctgcggccgc ctacggcgcg atcatcctgc
     6241 cgcgaaaccg cgacttctcg atgataacca tcccgcagag cgtgcggacg cacctgacgc
     6301 tgctcgcatc cgccggagcg tttctctggg gtataggcta cctgctcgac cgctacggca
     6361 ttcttttctc cccgcgcggc gtcaccttcg gcgcggggta cacggatgtc cacgcggagc
     6421 tcttcgcgct gaacgtcctc gcggtcatct ccatcctcat cgccgcagcg ctgcttgtaa
     6481 gcctcttccg caagatgtgg aagctctcgc tcggcatggt gggactgctc gtcgtctcca
     6541 gcgtcctcct gagaggcgtc tacccggcga tcgtccagaa gtacttcgtc gagccgaacg
     6601 agttcgagcg cgaacgccgc ttcatcgact acaacatcgc cgcgacgctc cacgcgtaca
     6661 acctgcacaa tgtggagctc aagacgatca cgcccgacga cgccatcacg tggcgcgaca
     6721 tcgaggagaa cagggacacc accgacaata tccgtttgtg ggatcacggc ccgctgctgc
     6781 ggagctacaa gcagctccag gagatccggc tctactatga cttcgcgaat gtggatatcg
     6841 accgctacaa gtttccctcc gtatcgggaa cgggctcgag cgaattccgg caggtgatgc
     6901 tcgcggcgcg tgagctggac atccgcggct tgcagaaccc gacgtggatc aacacacgcc
     6961 tcgaattcac ccacggctac ggcatcgtga tgaatccggt gaacgaggtc gccgggagcg
     7021 gccttccccg tctctgggtg cgcgaccttc ctccgagaag cgatgtggag cttcccatca
     7081 cgcatccgca gatctactac ggcgaaaagc ccagctccta catcttcgtc cggacgaccg
     7141 tgcaggagtt cgactacccg atgggcgact ccaacgtccg cacggtgtac gaggggaagg
     7201 gcggcgtgcc gatgggttcc ttcttccgcc gcattctctt cgcgctgaag ttcgccgata
     7261 tgaagatcct tctctccgac gtcttcaccg aggagagccg cgtgaagttc caccagaata
     7321 tccaggaacg cctcatgcgc gtggcgccgt tcctctactt cgaca
//
LOCUS       DAOPKU010000085         7360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10735_length_7360_cov_3.829432, whole genome shotgun sequence.
ACCESSION   DAOPKU010000085 DAOPKU010000000
VERSION     DAOPKU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10735_length_7360_cov_3.829432"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PK849_03905"
     CDS             complement(<1..714)
                     /locus_tag="PK849_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE65301.1"
                     /translation="MTYVYPGETSGRDPGKTPGQVPGEASGRVPGGASGQEPPEASGA
                     VSTGRRPALSGVTLRVREGEWLALLGSNGSGKSTLAKHLNALLTPTQGDCTVFGLSTD
                     DPQNVFAIRSAVSMVFQNPENQIVGTVVEEDAAFGPENQGLPTEEIVLRVENALRAAG
                     LLAKRKKPTYTLSGGEKARVGLAALLLQAPDLLLLDEPTNHLDFAALTWLEQYLQSYS
                     GSVLIVSHDRHFLNATVMTI"
     gene            complement(767..1120)
                     /gene="rplQ"
                     /locus_tag="PK849_03910"
     CDS             complement(767..1120)
                     /gene="rplQ"
                     /locus_tag="PK849_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPE65302.1"
                     /translation="MRHRMDSRSLGRYGSHRMAMLSNMAASLFLEENITTTLIRAKEL
                     RRFAERLITRAKGGTIHDRRIVRSKMAHKEAAIKLFDDIAKRYVNRPGGYTRIVKTGF
                     RAGDNASMAVISLVE"
     gene            complement(1125..2240)
                     /locus_tag="PK849_03915"
     CDS             complement(1125..2240)
                     /locus_tag="PK849_03915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPE65303.1"
                     /translation="MDYLRPEIRIEESTPKYAKVLIEPLERGYGATLGNSLRRVLLSS
                     IKGAAITSVRIDGVLHEFSTIPGVLEDVIEILLNLKHIPVRSFSQEVRVLRLETEGGG
                     LVTAANIQPDSEVEFVDPEAPICTMEEGARLSMDIYVEQGAGYASMERPRPAYLPVDA
                     LLVDAIFSPVLKVNYDVEAARVGQRTDFERLVLEIWTNGVHHPENTLCEASKILRGYF
                     GNIVEDMEKLNPVEPREQEAAAAGEGGVSEAAQERIAEEDDFLSRPVRDLELSIRSEN
                     CLLRGGIQTIGDLMGKNREDLLKIRNLGKISLKEIEEKMEKLGTIIASSKGEGKIREG
                     AGKSRGNAETNSQEDAAINTSKDEGKPQKGEVESKEE"
     gene            complement(2264..2896)
                     /gene="rpsD"
                     /locus_tag="PK849_03920"
     CDS             complement(2264..2896)
                     /gene="rpsD"
                     /locus_tag="PK849_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPE65304.1"
                     /translation="MSRYIGPACRLCKAEGTKLFLKGDRCYTAKCAITKSEGATVKGK
                     KGRPRTKVSEYGIRLREKQKLRRFYGMTELQFKRFFEIANKQTGQTGHNFLSLLERRL
                     DNVVYRLGFANSRKQARQLVLHGHFRVNGKRVDIPSYLVESGDLVAVMEKSREVTLIK
                     ENAEVATARKAPEWLEVNAEKIEGKVLSLPSREQIDAPVTEQLVVEFYAR"
     gene            complement(2930..3322)
                     /gene="rpsK"
                     /locus_tag="PK849_03925"
     CDS             complement(2930..3322)
                     /gene="rpsK"
                     /locus_tag="PK849_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPE65305.1"
                     /translation="MAKRTVRRGKKKERKNISYGVAHIYSTFNNTIVCVSDKGGNVLA
                     WASAGNVGFKGTRKSTPFAAQIAASQVAKAVQEHGVQEIDVVVKGPGPGRESAIRSLQ
                     AAGLQVNMIKDSTPVPHNGCRPPKRRRV"
     gene            complement(3355..3732)
                     /gene="rpsM"
                     /locus_tag="PK849_03930"
     CDS             complement(3355..3732)
                     /gene="rpsM"
                     /locus_tag="PK849_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPE65306.1"
                     /translation="MARIAGVDIPREKRVEIALTYIFGIGLPTSKKILAVTGVSPDTR
                     VKDLTEEESQKLRREIEDHYKVEGDLRREVSMNIKRLMDIGCYRGQRHKLGLPVRGQK
                     TKTNARTRKGPRRTVAGKKMATK"
     gene            complement(3739..3864)
                     /gene="rpmJ"
                     /locus_tag="PK849_03935"
     CDS             complement(3739..3864)
                     /gene="rpmJ"
                     /locus_tag="PK849_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPE65307.1"
                     /translation="MKVKPSVKPMCEFCRVIRRKGVVRIICSRNPRHKQRQGARR"
     gene            complement(3976..4197)
                     /gene="infA"
                     /locus_tag="PK849_03940"
     CDS             complement(3976..4197)
                     /gene="infA"
                     /locus_tag="PK849_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPE65308.1"
                     /translation="MSNKDEVIEVRGKVIEPLPNAMFKVELDNGHKILAHVSGKMRLH
                     FIRILPGDRVLVEISPYDLSRGRIIYRYK"
     gene            complement(4176..4601)
                     /locus_tag="PK849_03945"
     CDS             complement(4176..4601)
                     /locus_tag="PK849_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HPE65309.1"
                     /translation="MGMGLNELSDGGMCTIGQVVRSRKGKDAGRWYVVVGVAEDCGPS
                     RRTQGARVLVADGKKFTVGKPKAKNMIHLQRTRWVLDEIAHSILSKGNFDAGRFQALL
                     TGLANKNSIGGSVGCDPGNTASGASSGEDEETACPIKTK"
     gene            complement(4586..5362)
                     /gene="map"
                     /locus_tag="PK849_03950"
     CDS             complement(4586..5362)
                     /gene="map"
                     /locus_tag="PK849_03950"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPE65310.1"
                     /translation="MITLKKEREILLMRKAGKIVADVLDMIAEIVRPGVSTAEIDEAA
                     ESLIRKSGASPAFKGYRVPGIARPFPGTVCASINSEIVHGIPSRDRFLEEGDIISIDV
                     GTCFSGYYGDAACTYPVGRVSPVREKLLSVTKKSLEEALLVAQAGRTLGDIGHAVERC
                     VLESGYGIVRDYSGHGIGKYLHEPPQIPNYGQPGRGITLKSGMTLAIEPMVMTGSEDV
                     VTGSDQWVVKTADGSDAAHFEKSVLVKDGDPEILTPWAWA"
     gene            complement(5359..6015)
                     /locus_tag="PK849_03955"
     CDS             complement(5359..6015)
                     /locus_tag="PK849_03955"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPE65311.1"
                     /translation="MRILLLGSPGAGKGTLAAEIERKYPVAHISTGDIFRENVKNNTP
                     LGLEARAYLDSGKLVPDDIVIAMMEDRLREPDCEKGFILDGFPRTVPQAEALEKILEE
                     LRLELDGVVLLDVDEETVVRRLSGRRVCRSCGAIYNLFFRPSSKGELCEKCGGEIYQR
                     EDDREDVVRSRLKVFYKQTAPLIDYYDEKHMLSRIAAERSGAQHSLLEDLEQAVGISE
                     "
     gene            complement(6027..7318)
                     /gene="secY"
                     /locus_tag="PK849_03960"
                     /pseudo
     CDS             complement(6027..7318)
                     /gene="secY"
                     /locus_tag="PK849_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
BASE COUNT         1468 a         2207 c         1937 g         1748 t
ORIGIN      
        1 aatcgtcatc accgttgcgt tgagaaagtg ccggtcatgg gaaacaatta gcacactccc
       61 gctgtagctc tggagatact gttccagcca tgtcagcgcg gcaaagtcga gatgattggt
      121 cggctcatcg agcaacagca gatcgggcgc ttgcagcaac agcgcggcca atcccacgcg
      181 cgccttctca ccgccggaaa gcgtataggt aggcttcttg cgttttgcga gaagcccggc
      241 ggcacgcagg gcattttcga cgcggagtac gatctcttcc gtcgggagcc cctggttctc
      301 gggaccgaat gcggcgtctt cctcgaccac cgtgccgacg atctggtttt cagggttttg
      361 aaagaccatc gagacggcgc tgcgtattgc gaaaacattc tgcggatcat ctgtcgaaag
      421 gccgaaaaca gtacaatcgc cctgcgtcgg cgtcagcagg gcgttcagat gtttcgccaa
      481 ggtggatttg cccgatccgt tgctcccgag aagggcgagc cactcccctt cccgaacccg
      541 gagcgttacg ccggagagtg cgggacgtct gcctgttgaa accgccccgg aagcttcagg
      601 aggctcctgc cccgacgctc ctccgggaac tcgtcccgat gcctctccgg gaacttgccc
      661 aggcgttttc cctgggtcgc gtcccgacgt ttctccggga tagacatacg tcacatcccg
      721 gagttcgcat accgtcttgg aatccgtctt cataaaaggg tggtcattac tccaccaggg
      781 atatgaccgc catagaagcg ttgtcgcccg cgcggaaccc ggttttgaca atacgggtgt
      841 agccgcccgg acggttcacg tatctctttg caatatcgtc gaagagcttg atggccgctt
      901 ccttgtgcgc catcttcgag cgtacgattc tcctgtcgtg aatcgtgccg cctttcgccc
      961 gggtaataag acgctcggcg aatctgcgaa gctctttcgc acggataagg gttgtggtga
     1021 tattttcttc aaggaacaag ctggccgcca tgttcgacaa catggccatt ctatggctgc
     1081 cgtaccggcc aagggaccgg ctgtccatac ggtgtctcat ggaattactc ctccttcgat
     1141 tctacttcgc ctttttgcgg ctttccttcg tctttggacg tgtttattgc ggcatcctcc
     1201 tgggagtttg tctcggcgtt cccgcgggat ttcccggcgc cttccctgat tttgccttcg
     1261 cccttcgagc tcgcgatgat tgtcccgagc ttctccatct tctcttcgat ctccttcagc
     1321 gagatctttc ccaggttgcg gatcttcagg aggtcctccc tgtttttgcc catcaggtcg
     1381 ccaatcgtct gaataccgcc cctgagaagg cagttttcgc tccggatgga gagttcgaga
     1441 tcccggacgg gacgcgagag gaagtcatcc tcttcggcta tacgctcctg ggctgcctcg
     1501 gagacgccgc cctcgccggc tgctgccgcc tcctgctccc tgggttccac ggggttgagt
     1561 ttctccatgt cctccacgat attgccgaag taccctctca ggattttcga cgcttcgcag
     1621 agcgtattct cgggatgatg caccccgttc gtccagatct caaggacaag gcgttcaaag
     1681 tccgtgcgct ggccgacgcg cgccgcctcc acgtcgtagt ttactttgag gacaggggag
     1741 aagatcgcgt cgaccaacag ggcgtccaca gggagatagg ccgggcgcgg acgctccatg
     1801 gaggcgtacc cggcgccctg ttcaacgtag atatccatcg agagacgggc gccctcctcc
     1861 atcgtgcaga tcggggcttc cgggtcgaca aactccactt cgctgtccgg ctgtatgttc
     1921 gcggcggtga caagacctcc gccttccgtc tccagccgaa gcactcggac ttcctggctg
     1981 aaagagcgga cgggtatatg cttcagattc agcaaaattt cgatgacatc ttccagaaca
     2041 ccgggtatgg tgctgaattc atggagcacg ccgtcgattc gaacggacgt gatggccgca
     2101 cccttgatcg aggagagtag aactcttctg agagaatttc ccagagtcgc tccatatccc
     2161 cgctcgagcg gttcaatgag aaccttcgca tacttagggg tgctttcctc gatacggatc
     2221 tcaggccgca agtaatccaa ggcttccaac acctttcacg ccactatctg gcatagaact
     2281 cgacgacgag ctgctcggtc accggcgcat cgatctgctc acggctgggg agcgaaagaa
     2341 ccttcccttc gattttttct gcgttgacct caagccactc gggcgccttc cgcgcggtcg
     2401 ctacttccgc attttccttg atcagcgtga cttcgcggct cttttccatc acggcgacaa
     2461 gatcgccgct ttcgacgaga tagctgggga tgtccacacg cttcccgttc acacggaaat
     2521 gaccgtgaag cacaagctgt ctggcctgct ttctgctgtt ggcgaatccg aggcgataca
     2581 caacattgtc cagtctgcgc tcaagcaggc tgaggaagtt gtggcccgtc tgccccgtct
     2641 gcttgttggc tatctcgaag aagcgcttga actgcaactc ggtcatgccg tagaagcggc
     2701 gaagcttctg tttctcacga agacgaatgc cgtactcgct gaccttggta cggggacgtc
     2761 cctttttccc tttcactgtg gcgccttcgc tcttggtgat ggcgcactta gcggtgtagc
     2821 agcggtctcc cttgaggaag agctttgttc cctccgcctt gcacaaacgg cacgcaggtc
     2881 cgatatatct gctcattaga aataattccc tccttgacca aaaccagggc tagactctgc
     2941 gccgcttcgg cgggcggcag ccgttgtggg gcactggcgt cgaatccttt atcatgttga
     3001 cctgcaggcc tgccgcctgc agagaacgga ttgccgattc ccggccgggt cccgggccct
     3061 tgacgacgac gtcgatctcc tggacgccgt gttcctgcac agccttggcg acctgcgacg
     3121 cggcgatctg cgccgcgaag ggggtggact ttctggtgcc cttgaatccg acgttcccgg
     3181 ccgaagccca ggcgaggacg tttccgccct tgtcgctcac gcacacgata gtgttgttga
     3241 atgtggagta gatgtgcgcg acgccgtagc taatattctt tctttccttc ttcttacctc
     3301 tgcggactgt tcgcttggcc acttgttcca gcctccttgt tctctatccg aaggctactt
     3361 cgtagccatc tttttgcccg ccaccgtgcg gcgagggcct ttgcgggtgc gcgcgttcgt
     3421 cttggtcttc tgtccgcgga ccggaaggcc gagcttgtgg cgctgtcctc tgtagcagcc
     3481 gatgtccata agacgtttga tgttcatgga cacctctctg cgaaggtcgc cttccacctt
     3541 gtagtgatcc tcaatctccc tgcggagttt ctgggactcc tcctcggtaa gatccttcac
     3601 gcgcgtgtcc ggacttactc cggtgacggc gagaatcttc ttcgaggtgg gaaggccgat
     3661 tccgaaaata tatgtcaacg ctatctccac acgcttctcg cggggaatat caactccggc
     3721 gatacgggcc attcgcgctt acctccttgc accctgtctc tgcttatgcc ggggattcct
     3781 gctgcagata atccgcacga cacccttacg ccggattacc cggcaaaact cgcacatcgg
     3841 ctttaccgat ggttttactt tcattgattc cacgctcctc tgaaagaact gaaacagagg
     3901 cccttcaggg cttctgaaaa tatcttttct ccaagcggtt tattatacag gagatcggac
     3961 aatatgaaaa tactcttatt tatatctata gatgattctg cccctggaca ggtcgtacgg
     4021 agagatctcc accagaaccc tgtcccccgg gaggatgcgg ataaaatgaa ggcgcatctt
     4081 tcccgaaacg tgggcgagta tcttgtgtcc gttatcgagc tccaccttga acatcgcgtt
     4141 tggaagcggc tcgattactt ttcctcgtac ttctattact tcgtctttat tggacatgcg
     4201 gtctcctcat cctccccgga ggaggcgccg gacgctgtgt ttcccggatc gcagccgact
     4261 gaccccccga tactgttctt atttgcaagc cccgtaagca gagcctgaaa ccttcctgcg
     4321 tcaaagttac ctttcgacag gatactgtgc gcaatttcgt caaggaccca tcgcgtccgc
     4381 tggaggtgga tcatgttttt tgccttgggt ttcccgacag tgaacttctt tccgtccgcc
     4441 accaaaaccc tcgctccctg cgttcttcga gatggcccgc agtcctctgc aacgccgact
     4501 acgacgtacc atcttccggc gtcctttcct ttccgagatc tgaccacttg gccaattgtg
     4561 cacatcccgc catcggaaag ttcattcagg cccatgccca cggcgtcagt atctccggat
     4621 ccccgtcttt gacgagaacc gacttttcaa aatgagcagc atccgagccg tcggccgttt
     4681 tgacaaccca ctggtcgctt ccggtgacaa catcttcgga acccgtcatc accattggtt
     4741 caatggccag cgtcatcccg gacttgaggg tgatccctct tccgggctga ccgtagttcg
     4801 gtatctgggg cggttcgtgc agatacttcc ctataccgtg tcccgaatag tcccggacaa
     4861 taccataacc gctctccaac acgcagcgtt ccaccgcgtg tccgatatcg ccgagcgttc
     4921 tgccggcctg cgcaacgaga agcgcttctt caagactctt cttcgttacg gagagaagtt
     4981 tctccctcac cgggctcact cttcccacgg ggtaggtaca tgcggcgtcg ccgtaatatc
     5041 cggaaaaaca ggttccgaca tcaatactga tgatatcgcc ttcctccagg aagcggtcgc
     5101 ggctgggtat tccgtggacg atctcggagt tgatggaagc gcagactgta ccgggaaaag
     5161 gccgtgctat tcccggtacc ctgtaccctt tgaaggcagg ggaagccccg gactttctga
     5221 tgagagactc cgcagcctcg tcaatctccg cggtggatac cccgggacga actatctcag
     5281 cgatcatgtc gagcacgtcc gccacaattt ttcccgcttt cctcataagg aggatttccc
     5341 gttctttttt caaggttatc attcggaaat gccgaccgcc tgttcgagat cttcaagcag
     5401 cgaatgctgc gcgccgcttc gttccgcagc tatacgtgaa agcatgtgtt tctcgtcgta
     5461 gtaatctatc agaggcgcag tctgcttata gaagaccttg agacggctgc ggaccacgtc
     5521 ttcccggtcg tcctccctct gataaatctc ccctccgcac ttttcgcaga gttctccttt
     5581 cgacgagggc ctgaaaaaga ggttgtagat ggcgccgcag ctccggcaga ctcttctgcc
     5641 ggagagccgg cgtacaacgg tttcctcgtc gacgtcgaga agcactactc cgtcgagttc
     5701 caaacggagc tcttcaagta tcttttcaag agcttcagcc tgggggacgg tgcggggaaa
     5761 cccgtcgaga ataaatccct tctcgcagtc gggctcccgc aggcgatcct ccatcatagc
     5821 aatgacaatg tcgtcgggca ccagtttgcc cgaatccaaa tacgctcgag cttccaatcc
     5881 aagtggagta ttgttcttca cgttctctct gaagatgtcg ccagtggaaa tatgtgcaac
     5941 gggatattta cgctcgattt ccgccgccag tgttcccttg cctgctccag gtgaacctag
     6001 aagaagtatc cgcatttttt ctctccttag aatcgaagta agccgccggt cttgtccttg
     6061 cgtttcaata tcccttcata gtgacgcata agaagctgtc cttcaatctg atggaccgta
     6121 tcaagcgcca caccgacgac gatgagaacc gcggtgccgc caaagtagaa gctcgtgatt
     6181 cccatgaact gggtcataag gttcggcacg agtgcgacga acgcaaggaa aactgctccc
     6241 gcgagcgtaa ttcggctcat aaccttttcg ataaaatccg atgtgggttt tcccgggcgg
     6301 atgccgagga tgaacccgcc gtacttcttc atgttgttcg ccacatcggc ggggttgaag
     6361 acgaccgccg tgtagaagta ggagaagaag atgatgagca caacgtaaaa gatcgtgtag
     6421 acaaagctgc ccggcccgaa gatacgctgg aagaacaggg cgatatcccc ggagaagaac
     6481 ttcgcgatcg aatacgggaa aagaagcacg gacgacgcga agatgatggg gataactccc
     6541 gcctggttga ccttcagcgg gataaacgtg ctctgtccgc cgtagacctt gttgccgaca
     6601 acacgcttcg catactgcac aggaaggcgt ctctgcccct cctggagcat gatgcatccg
     6661 gcgatgacgc cgaccataag cacaagcgca agcagcaata cgaggaaatt catctcgccg
     6721 agccggatca tgttccagct ctggatgatc gcctcaggca ccctcgccac tattccggcg
     6781 aagatgagca gtgagattcc gttgccgatt ccgtggtcgg aaatttcctc tccgagccac
     6841 atgacagcaa tggctccggc agtcaccgta catactacga tggcgccctc aaaaagaaaa
     6901 ccgctgaaaa ttccgaggcc gcgaagccag aaggtcatac cgatggcctg gacaagcgcg
     6961 aagccaacag cggcgaggcg cgtccactgc acgatcttct ttcttccatc ctcgccttcc
     7021 ttctgcatct tttcgagggt ggggaagatc acgacgagca gctgcataac gatgctcgca
     7081 ttgatgtacg gcgcgacgcc gagggcgaag acgctgaatc ttctgagcgc tccgcccgcg
     7141 aatatgtcga ggaatcccag gactcccccc tggtcgaaga ggcttgacat tgcctccgca
     7201 tcgattccag gggttggtac atgcgctcca agcctgaata tcaggagcac tcccagggta
     7261 aaagaattct tcgtttgaga tccggaagtc gaaatgcatc cctgaaggag tcaagcactt
     7321 agatcacctc ggccttccca cccgcggctt ctattttcgc
//
LOCUS       DAOPKU010000086         7293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10871_length_7293_cov_4.938657, whole genome shotgun sequence.
ACCESSION   DAOPKU010000086 DAOPKU010000000
VERSION     DAOPKU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10871_length_7293_cov_4.938657"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PK849_03965"
     CDS             complement(<1..899)
                     /locus_tag="PK849_03965"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HPE65312.1"
                     /translation="MKEKPHEITQKIRFFNRFYTRIIGAVNQSVLNSPFSMAEVRVLF
                     EIKHSDNPTASEVNRELELDPGYLSRIVRRFEQDELLTKERSPLDGRAYLLKLTEHGE
                     RVLASLEKASDKKAADLISPLSEREITALLRSMETIHSLLTKTPLELEVRPAAPGELG
                     TIVARHMDYYSKQYAFDASFESYLFDGIARFLKNRENGMGQAWVVKHGGVVVGSIAID
                     HVEDRVAQLRWFLVEPEYRGMGMGRRLIEEALDFSRMNLYHRIFLWTFSELRNARHLY
                     EMYGFSPTEEVTHTIWGRELTEER"
     gene            complement(1049..2323)
                     /locus_tag="PK849_03970"
     CDS             complement(1049..2323)
                     /locus_tag="PK849_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPE65313.1"
                     /translation="MADATPFLRGLRGATILGINPPVFDFTFFDLWAKPLGLLFLLDA
                     LRRRGNTVHLLDCIAEGSAEQISFGRRKLLRREISKPAPFRNIPRRYHRFGLDDAEIL
                     LRLHALPRPDCIMVTSGMTYWYEGVFRAVELAKEVFPGVPVLLGGVYARLCPEHAGRS
                     GADIVQDILPDIPFVRPAMELYSEPGYALLLTSFGCPLSCEYCASKLLFPQFIERDLS
                     ETLADAAFQFSLDGVHDAAFYDDALLIDKERRFYPLCEELVRRFPGVRYHTPNGLHIR
                     EIDERCAAVLKQTGFHTLRLSLESSDPAFQRKGSGKTSGEEFQHALRNLQKAGFAPEQ
                     METYILAGLPGQTTVSVEASIRFVESCGARAKLAEFSPVPGTPSFEEAASILPELRSE
                     PLLHNKTVYSSFLSGNISPEKLQFLKDLTKHR"
     regulatory      2495..2603
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2641..3156
                     /gene="thiT"
                     /locus_tag="PK849_03975"
     CDS             2641..3156
                     /gene="thiT"
                     /locus_tag="PK849_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HPE65314.1"
                     /translation="MFHVRNTILVEGALAVALSVVFSALRLWTMPQGGSITLEMLPIF
                     LFALRRGGKAGCAAGAVSGVMQLITGGYIVHPAQALLDYPLAFGVLGVTGFFKNRPLW
                     LGITLGGVLRFFCHVLSGVVFFGSFAPEGTNVWMYSAVYNGSFMAPTLVVCGVLAYLI
                     WPRLRRVGAEG"
     gene            complement(3131..4213)
                     /locus_tag="PK849_03980"
     CDS             complement(3131..4213)
                     /locus_tag="PK849_03980"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPE65315.1"
                     /translation="MVHSSPNITLEEGAAPLLRWYDACRRPFPWRETNDPYAVWISEV
                     MLQQTQAARGVEYHRRWMRIFPTLRSLAEAKDDDVLKAWEGLGYYSRARNLLRAARLL
                     QARGLATLPASFEELRALPGIGPYTAAAVASIAFGLPVPALDANGKRVFSRLLDLEEP
                     VDRRAGEKSVLRAFVQMLPADRPGDFNSAIMELGATLCAPTSTLCGECPLEFLCLARL
                     RGSALERPVLSRQTKSESLEGILAVFVSGDRVFLRRRPPAGLWANFEEFPWSAPPAEQ
                     ESPLTELARRSNADCGGEVRCSFTKWRLRLAVRIVRLHAPGDLENMNGRWASPSELAA
                     ATLPAGSRKMRERLFGEGTIPPPPPS"
     gene            4406..6433
                     /gene="glgB"
                     /locus_tag="PK849_03985"
     CDS             4406..6433
                     /gene="glgB"
                     /locus_tag="PK849_03985"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPE65316.1"
                     /translation="MAKTENIRYDFSLFGEMDSYLFREGNHFRLYEKLGAHVVDDGKL
                     PGTYFALWAPNAREVSVIGAFNDWDRTRHYLSPRKDNSGIWEGFVPGVGKGALYKYSL
                     RSRNGSVFEKGDPFAAFWEIAPKTASIVWPCDYAWNDGEWMKNRRAQNALNAPMSVYE
                     VHVGSWRRKPAEGFRSLSYRELGHELGEYVRAAGFTHVELLPVMEHPFYGSWGYQTLG
                     YFAPTSRYGTPEDFMYMVDVLHQMGIGVILDWVPSHFPADAYGLAHFDGTCLYEHEDP
                     RKGYQPDWGSQIFNFGRNEVREFLISSAFCWLDLFHADGIRVDAVASMLYLDYSRKEG
                     EWIPNQFGGRENLEAVGFLKKMNEAIYQEFPDVQTIAEESTAWPMVTRPTFIGGLGFG
                     MKWNMGWMHDILEYMQKDPVYRKYNHNKITFSIMYAFTENFLLPFSHDEVVHGKGSML
                     GKMPGDEWQQYANLRLLLGYMYAHPGKKLLFMGSEFGQGREWNHDDSLFWDLLHSPRH
                     KGMLDWVTDLNRVYRSEAALHSRDFSPEGFQWCDFSDWEQSVVSFVRKGAGGEMVFAV
                     CNFTPVPRFGYRISVPEGGWWRELLNSDAAEYGGGGVGNFGGAKAEPIPNSSWHSLTI
                     SLPPLGMLLFKLERPEAFPEAVAANRPVSIPVTLPEPAKPPLPEATRRGKK"
     gene            complement(6496..>7293)
                     /locus_tag="PK849_03990"
     CDS             complement(6496..>7293)
                     /locus_tag="PK849_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE65317.1"
                     /translation="ARMAEAPPLVIAAYRVGLATLFLLPFTARRAVREISSLDRSDIL
                     RGVASGVFLALHFATWISSLFYTSVASSVVIVNAIPLWTGLLSPLMTGDPFSPSLKKG
                     LLLALPGAVVISWGDFALGWSALWGNFLALQGSFFAALYILFGRKVRPRISLGSYVTL
                     NYGTAAVVLWIVVLAFGLPVAGYSTGTWSAFFLMALVPQLLGHSSYNWALRWLTGGTV
                     ALCLLGEPVGSSLLAALFLGEPITLVKVLGGGLILAGIYLASKESGE"
BASE COUNT         1438 a         2156 c         2264 g         1435 t
ORIGIN      
        1 cgttcttcgg tcagttcacg cccccaaatc gtgtgcgtga cctcctcagt gggggagaat
       61 ccgtacatct cgtagagatg gcgtgcgttc cgaagttcgg aaaacgtcca caggaagatc
      121 ctgtgataca ggttcatccg gctgaaatcc aaagcctctt cgatgaggcg cctccccatt
      181 cccatcccgc ggtactccgg ttctacgagg aaccagcgga gctgggcaac ccgatcttcg
      241 acatggtcta tcgcgatgga gccgacaacc acaccgccgt gcttcacaac ccaggcctgc
      301 cccatgccgt tctccctgtt cttcaggaaa cgggctattc catcaaagag gtaggactca
      361 aacgacgcgt cgaatgcgta ctgtttgctg taatagtcca tgtgacgcgc aacgatcgtg
      421 ccgagttcgc ccggagctgc aggccggact tcgagctcaa gcggagtttt cgttaaaagt
      481 gagtggatgg tctccataga gcgcagcaac gctgtgattt ctctctctga aaggggggag
      541 ataagatccg ccgctttctt gtccgacgcc ttttcgagag aggcgagcac acgttcgcca
      601 tgttcggtca gcttgagcag atacgctcgt ccatcgagag gcgaacgctc cttcgtcagc
      661 agttcatcct gttcaaagcg gcggactatc ctgctcaagt accccgggtc gagttcgagc
      721 tccctgttga cttcgcttgc cgtagggtta tcggagtgtt ttatctcgaa gagcactctc
      781 acctccgcca tggagaatgg actgttcaac accgactggt tgacggcgcc gatgatccgt
      841 gtataaaacc tgttgaaaaa acgtatcttc tgtgtgattt cgtgcggctt ctctttcact
      901 tccatcacat cctttcgttt tggattttca ctctgtaatg atatttctta tcattgatgc
      961 tgtcaagcaa attatcgccc gtttggaaga aattttggtg gaaatacaac cctttcggtg
     1021 aggagctcga gatgtgtatg cacatatatc atcgatgctt cgttaaatcc tttaggaatt
     1081 gaagtttttc gggagagata ttccctgaga ggaatgacga gtagaccgtc ttgttgtgca
     1141 gcagcggctc gctccgaagt tcgggaagaa tggaggcggc ttcctcgaac gagggcgttc
     1201 ccggcacagg ggagaattcc gcaagctttg ccctcgctcc gcatgattcg acgaagcgta
     1261 tcgacgcttc tacggagacg gtcgtctgcc cgggaaggcc ggcgagaatg tacgtttcca
     1321 tctgttcggg tgcgaagccc gccttttgaa ggttgcgaag ggcgtgttgg aactcttctc
     1381 cggacgtctt gccggagccc tttcgttgaa aggcggggtc gctgctctca agactcaggc
     1441 gcagcgtatg aaaccctgtt tgcttgagga cagcggcgca gcgttcgtcg atctcccgga
     1501 tgtggagccc gttcggcgta tggtagcgaa cgccggggaa acgacggacg agttcctcgc
     1561 agagagggta gaagcgccgt tccttgtcga tcagcagcgc gtcgtcgtag aatgccgcgt
     1621 catgcactcc gtcgagggag aactgaaagg cggcgtcggc aagcgtttcg gaaaggtccc
     1681 tctcgatgaa ctgcgggaag agcagcttgg aagcgcagta ctcgcaggag agcggacagc
     1741 cgaacgacgt cagaagcagc gcgtaccccg gctcggagta cagctccatc gccgggcgga
     1801 cgaaggggat gtccggcagg atgtcctgta cgatgtccgc tcctgaacgt ccggcgtgtt
     1861 cggggcagag ccgggcatac actcccccca aaaggacagg aacgccgggg aagacctcct
     1921 tcgccagttc gacggctcga aagacgccct cataccagta ggtcataccg gacgtgacca
     1981 tgatgcagtc cggacgcggg agcgcatgca gacgcaggag aatctccgcg tcgtccaagc
     2041 cgaacctgtg gtagcgccgg gggatgttcc ggaagggagc cggtttggag atttccctcc
     2101 ggaggagctt cctgcgtccg aaggagatct gctcggcgga gccttccgcg atgcagtcga
     2161 gaagatggac tgtattcccc cgtctcctga gagcgtcgag gagaaagagg agcccgagcg
     2221 gtttggccca caggtcgaaa aacgtgaagt cgaagacggg gggattgatc ccgagaatgg
     2281 tcgctccccg aagccccctg agaaacggcg ttgcatccgc catgaatgat cagctctttt
     2341 cagaagatat ggaggaagtc ttggcggctt ctccctcttt aataatacca tcgcgccgac
     2401 ggacgcaacg aaggcgccgc gtcggcgaac ctctccccgt gcagagagtc cggccgtttt
     2461 tgacagtatt tcttggcaag gtgtataatt gcggcgttcg catcggggga gtccgcaacg
     2521 gactgagagg ggggtgttcg tcccccgact cctggaacct gatccgggtc atgccggcga
     2581 agggaaggtg ccgctttcag ggacattcca ccgatgaaaa gttgaaagga ggagtgttcc
     2641 atgtttcatg tccgcaatac tattctcgtc gaaggcgctc tcgcagtcgc cctctccgtt
     2701 gtgttctcgg cgcttcgtct ctggacgatg ccccagggag gctccatcac cctcgaaatg
     2761 cttccgatat ttcttttcgc cctcaggcgg ggcggaaaag caggatgtgc ggcgggagcc
     2821 gtctcgggcg ttatgcagct catcaccggc gggtacatcg tgcaccccgc acaggctctt
     2881 ctggactatc cgctcgcgtt cggcgtcctc ggcgtaacgg gtttcttcaa aaaccgtcct
     2941 ctctggctgg ggataacgct cggcggcgtg cttcgcttct tctgtcacgt tctctccgga
     3001 gtcgtcttct tcggctcgtt cgccccggaa gggacgaacg tctggatgta ctccgcagtc
     3061 tacaacggct cgttcatggc cccgacgctc gtcgtctgcg gcgtcctcgc ctatcttatc
     3121 tggcccaggc tcaggagggt gggggcggag ggatagttcc ctcgccgaag agccgctccc
     3181 gcatctttct gcttccggcg ggcagagtcg ccgccgccag ttccgacggg cttgcccacc
     3241 tgccgttcat gttttcaagg tcgccgggag catgcagccg tacaatccgg actgcgaggc
     3301 gaagacgcca cttcgtaaag gagcagcgaa cttcgccgcc gcagtccgcg ttgcttctgc
     3361 gcgccagttc ggtcaacggc gattcctgct ccgcaggagg cgcgctccag ggaaactcct
     3421 cgaagttcgc ccacagtccc gccggggggc gtcttcgcag aaaaacgcga tctccggaca
     3481 cgaagacggc aaggattcct tcgaggctct ccgacttcgt ctgccgggag agtaccgggc
     3541 gttcgagggc gcttccccga agccgcgcga gacagaggaa ttcgagcgga cactctccgc
     3601 acagggttga ggtaggcgcg cagagcgtcg cgccgagctc catgattgcg gagttgaagt
     3661 cccccggacg atccgccgga agcatttgca caaaggccct caggacactc ttttcccctg
     3721 ccctgcggtc gaccggctct tcaaggtcga gaagccggga gaagacgcgc ttcccgttgg
     3781 cgtcgagggc tggaacggga agaccgaagg cgatgctcgc cacggccgcg gcggtgtagg
     3841 ggccgatgcc cggaagggcc cgcagctcct cgaaggacgc gggaagcgta gcaagtccgc
     3901 gcgcttgcag cagccgcgcc gcacgaagca gatttctcgc tctggagtag tagcccagcc
     3961 cttcccatgc tttgagaacg tcatcgtcct tcgcttcggc gagcgagcgc agcgtgggaa
     4021 agatgcgcat ccagcggcga tggtactcca cgcctcgtgc ggcctgcgtc tgctggagca
     4081 tcacctccga gatccagacg gcgtacggat cgttcgtctc ccgccaggga aagggccgcc
     4141 ggcaggcgtc gtaccaccgc agcaacggcg ccgccccttc ttccagagtg atgttcggcg
     4201 aactgtgtac catgcagcgc gctccttcct tcttcatctc ttcgtttttc ttccatagag
     4261 catatgtata acatggagcg tcatagggta caatacgagg ggagtattcc ggatatttca
     4321 ccggctccga aaagccctgg ccatagaggc tttccccccg ttcgttcgag ggggcgcgct
     4381 ctatcccgca gaaggaggaa cggcaatggc gaagacagaa aacatacggt acgatttttc
     4441 tcttttcggc gagatggact catatctctt ccgggagggc aatcacttcc gcctgtacga
     4501 aaaactggga gcgcacgtcg tcgacgacgg gaaacttccg gggacgtatt tcgcgctctg
     4561 ggcgcccaac gcgcgggagg tcagcgtgat cggcgccttc aacgactggg acaggacgcg
     4621 ccactatctc tccccgagaa aggataactc cgggatatgg gagggctttg ttcccggagt
     4681 ggggaagggc gcgctgtaca agtattccct ccgctcccgc aacgggagcg ttttcgagaa
     4741 gggagatcct ttcgccgcct tctgggagat cgcgccgaag acggcctcca tcgtctggcc
     4801 ctgcgactat gcctggaacg acggggagtg gatgaagaac cgccgcgcgc agaacgctct
     4861 caatgccccg atgtcggtct acgaggtgca tgtcgggtcg tggcgcagaa aaccggccga
     4921 gggcttccgt tcgctctcct accgcgagct ggggcatgag ctcggcgagt acgtccgcgc
     4981 ggccggcttc acgcacgtcg agctgctccc cgtcatggaa cacccgttct acggctcctg
     5041 ggggtatcag acgctgggct acttcgcccc taccagccgt tacggaacgc cggaggactt
     5101 catgtacatg gtggatgtgc tgcatcagat ggggatcggc gtgatcctcg actgggtgcc
     5161 gtcgcacttt ccggccgacg cgtacggtct tgcacacttt gacggcacct gcctctacga
     5221 gcacgaggat ccccggaagg ggtaccagcc ggactggggc agccagatat tcaatttcgg
     5281 ccgcaacgag gtgcgggagt tccttatcag cagcgccttc tgctggctcg acctctttca
     5341 cgccgatgga atacgggtgg acgccgtcgc ttccatgctc tacctcgatt actcccgaaa
     5401 ggaaggggag tggatcccga accagttcgg cggcagggag aatctggaag ccgtcggctt
     5461 tctcaaaaag atgaacgagg caatctacca ggagttcccc gatgttcaga caatcgccga
     5521 ggagtcgacg gcctggccga tggtgacgcg gccgaccttc atcggaggac tcggcttcgg
     5581 catgaagtgg aacatggggt ggatgcacga cattcttgag tacatgcaga aggaccctgt
     5641 ctaccgtaag tacaaccaca acaagatcac cttcagcatt atgtacgcct tcacggagaa
     5701 ctttctcctc cccttctctc acgacgaggt ggttcacgga aaagggtcca tgctcggcaa
     5761 gatgccgggc gacgaatggc agcagtacgc caatctaagg ctgctgctgg ggtatatgta
     5821 cgcgcatccg gggaagaagc tcctcttcat ggggagcgaa ttcggccagg ggagggagtg
     5881 gaaccacgac gacagcctct tctgggatct gctgcactct ccccgccaca aggggatgct
     5941 ggactgggtg acggatttga accgggtcta caggagcgaa gccgccctcc attcgcggga
     6001 cttctcgccg gaggggttcc agtggtgcga cttctccgac tgggagcaga gcgtcgtctc
     6061 cttcgtcagg aagggcgccg ggggggagat ggtctttgcg gtctgcaact tcacccctgt
     6121 gccgcgattc gggtaccgaa tctccgtccc ggagggcgga tggtggaggg agctgctcaa
     6181 cagcgacgcg gcggagtacg gaggaggcgg cgtcggcaac ttcggcggcg cgaaggcgga
     6241 gccgataccg aactcctcct ggcattcgct gactatttcg ctgccgccgc tgggaatgct
     6301 gctcttcaag ctggaacgac cggaagcgtt tccggaggcc gtcgcggcaa atcggcctgt
     6361 gtccatcccc gttacgctcc cggagccggc aaaaccgcct ctgccggagg cgacgagacg
     6421 agggaaaaag taacatcata cagcgcggag gagacacgac gttctcctcc gcgttttttc
     6481 ttcgtcgaat cgcgtctatt ctccggactc ctttgacgcg aggtagatgc ccgcaaggat
     6541 gagccctcct ccgaggacct tgacgagcgt gattggttcc ccgaggaaga gggcggcgag
     6601 cagcgagctc cccacgggct cccccagcag acagagcgcg acggtgccgc ccgtcagcca
     6661 gcggagcgcc cagttgtagc tcgaatgccc cagcagctgc ggcacgagcg ccatgaggaa
     6721 gaacgcgctc cacgttcctg tcgagtagcc ggcgacgggg aggccgaagg cgagcacgac
     6781 gatccagagg acgactgccg ccgtgccgta gttcagcgtc acgtagctcc ccagcgagat
     6841 gcgcggcctg accttcctgc cgaagagaat gtacagagcg gcgaagaagc tcccctggag
     6901 cgcgaggaag ttcccccaca gcgcggacca ccccagagcg aagtcgcccc agctgatgac
     6961 gacggctccc ggaagcgcca gcagcagccc cttcttcagc gacggggaga acgggtctcc
     7021 cgtcatgagg ggcgagagca gccccgtcca cagcggaatt gcgttcacga tgaccacgct
     7081 gctggccacg gacgtataaa agagcgagga gatccacgtg gcgaagtgga gcgccagaaa
     7141 gaccccggaa gcgacgccgc gcagaatgtc gcttctgtcg agcgaggaga tctcgcgcac
     7201 cgcccgccgc gccgtgaatg gaagcagaaa gagcgtcgcc aatccgaccc tgtaggcggc
     7261 gatgacgagc gggggcgctt cggccatccg tgc
//
LOCUS       DAOPKU010000087         7270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10903_length_7270_cov_5.207762, whole genome shotgun sequence.
ACCESSION   DAOPKU010000087 DAOPKU010000000
VERSION     DAOPKU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10903_length_7270_cov_5.207762"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PK849_03995"
     CDS             complement(<1..359)
                     /locus_tag="PK849_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPE65318.1"
                     /translation="MSNFIEEFPGTTLILAGGQGSRMGGEKLTLAVDSEPLLLKVIRR
                     VLPFSKDILLSVAPRQVSYVSTVLSGIISLYGIRTVEDRLPVEKSSSEVDEKRSGPLA
                     GIVSGLEACSSEWLFVCA"
     gene            461..1189
                     /locus_tag="PK849_04000"
     CDS             461..1189
                     /locus_tag="PK849_04000"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPE65319.1"
                     /translation="MEKKKFLYEGKAKKIYDTDDVNLCIIEYKNSLTAFNALKKDTIE
                     GKGELNNLISSSLFELLSEKGIPTHFVKRVDNIHQIVKKVSIVPIEVVVRNITTGSLC
                     KRLGVEEGKVLPRPLVELYLKDDALGDPLITEDHALLFGWSTEEQLLKIKEIALKVNS
                     VLSEYFTGLRIILVDFKLEFGVDAEGRLLLADEVSPDTCRFWDRDTKNRLDKDRFRKD
                     LGDVLGAYQEIWRRISSSNEDGAR"
     gene            1186..1437
                     /gene="purS"
                     /locus_tag="PK849_04005"
     CDS             1186..1437
                     /gene="purS"
                     /locus_tag="PK849_04005"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPE65320.1"
                     /translation="MTYRVSIFIELQEGVLDIQGKAVERSLAGMGHDALRSVKVGKYI
                     QVWVETGSAEDARKEAERMCDDLLVNSVIEQFRIEVEGE"
     gene            1438..2142
                     /gene="purQ"
                     /locus_tag="PK849_04010"
     CDS             1438..2142
                     /gene="purQ"
                     /locus_tag="PK849_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPE65321.1"
                     /translation="MNTAVIVFPGSNCDQDVMHAVAGSLGTKVRAVWHKETTLPEDTD
                     FVILPGGFSFGDYLRCGAMAARSPIMKAVAEHGRRGGLLLGICNGFQILTESRLLPGA
                     LLPNRTLNFVCRPCFLRVESVSTPFTSLFSKGQVVQFPIAHGDGLYTLPEEELKALED
                     RGGVVFRYSDSDGLTGDDANPNGSANNIAGIVNERGNILGLMPHPERASEALTGGEDG
                     RVFWQSILESFTKGGR"
     gene            2144..4294
                     /gene="purL"
                     /locus_tag="PK849_04015"
     CDS             2144..4294
                     /gene="purL"
                     /locus_tag="PK849_04015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPE65322.1"
                     /translation="MEYTQAGLRETEYEEIRRVLGREPNECELRIMGVMWSEHCSYKS
                     TKNLLKLFPNNGDRVVLGPGENAGIIDLGDGLGAAFKAESHNHPSAVAPYQGAATGVG
                     GIIRDILALGARPVASMDGLFFGEPSSPRTQSLASGIVHGVGGYGNAVGVPTVGGKTA
                     YDPSYNENPLLNAFCIGLIPLDEIVSSRTARPGQAVVLLGSKTGRDGIAGAAFASVEL
                     SEDNKASRPSIQIGDPFGEKLLIEACLEMNEKKLIVSMQDMGAAGIISSSSEVAAKSG
                     VGMILHFDKIPLRAENMEPWEIALSESQERMLLIVDRSYLHEVMEVAEKWELDCAEFG
                     ETVEGDEYTILFHGERAASLPATLIGDRCPLIPWPSRRPADLEKKWEFDVDDIPLPED
                     WNETLLSLLADPSLASKKRIYEQYDSMVQLNTVIGPGHPVSMLRIKGRDSLIAMTLDA
                     DPWKCSLDPFRGGAETVARTVRDLAVAGARAAGLTDCLNFPSPEVPEQYWVLEECVKG
                     MAEACGAFDCPVVSGNVSLYNESPDGAILPTPVVGTVGIIPSIDAALSAGTWEEGDVL
                     FLVGPCNPSLAGSRYLLSQGKLAGRPLPFYPEAEKDFVERALKTASAKAAHSGRALSG
                     GGLAAALAKESMESGIGASISLSIPTRKDILLFGEGCARALYAVPMSRVPLFKVLWNG
                     YPCIQLGRVGGERLSVEGAFSLTVSELLSKWRNG"
     gene            4295..5668
                     /gene="purF"
                     /locus_tag="PK849_04020"
     CDS             4295..5668
                     /gene="purF"
                     /locus_tag="PK849_04020"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPE65323.1"
                     /translation="MCGVFGAFSPDGKKVLEDVYLGLYALQHRGQEAAGVAWTNATGG
                     ISSRKGNGLVHLALDQADLAAVESSCAIGHVRYSTTGGSELSNAQPLTASTSKCPIAV
                     AHNGNLTNAQGLKLLLQNRGAIFHSTTDTEVILHLIAHQPHKQPLDALLDSLVRLRGA
                     YSLAMILGNRLVAARDPWGFRPLVLGRRDNIHYVSSETCALNLVGASVVREIEPGEVI
                     IIGEDGVHSIRLPVEPKRRFGCAFEFVYFARPDSVISGRSVYAVRKELGKKLAKRAPC
                     REASLVTGMPDSGTIASLGYAEESGVPYEQSVVRNRYVGRTFIEPTSRVRHLGVRIKL
                     NPITEVLQGKNVVVVDDSIVRGTTCERMISMIKSCGANQVHVRISSPPVRFPCYYGID
                     TPTRVELAAARMNLQQLEQEVGADSLAYITEEDLLEAIGLPAQSVCTACFSGDYMEGG
                     EEYEPQL"
     gene            5652..6650
                     /gene="purM"
                     /locus_tag="PK849_04025"
     CDS             5652..6650
                     /gene="purM"
                     /locus_tag="PK849_04025"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPE65324.1"
                     /translation="MNLSYKNAGVDISLADKWVDVIKGISKSMPAKKEIIGGIGGFSG
                     LYRLPGGMALAGCCDGVGTKIEIARAAGMFTHIGQDLVAMNVNDLITCGARPLFFLDY
                     IACGKLNIPVLQPIVESVARACGECGCALLGGETAEMPGVYPEDGLDLAGFAVGIVEE
                     EKIIDGKNVAAGDAVVGIPSSGVHSNGFSLVRRALFSEGTPYSLDEAMPRFGGATLAE
                     ILLAPTKLYVNTALRAVSTGKVKAMAHITGGGLADNLRRVVPDGLGLSLDYTAWPRPA
                     IFNLLSEEGVEEEEMRRVFNLGIGYCLIIAQEDVSLVLEQIEAQREQGIIIGEVVA"
     gene            6647..7240
                     /gene="purN"
                     /locus_tag="PK849_04030"
     CDS             6647..7240
                     /gene="purN"
                     /locus_tag="PK849_04030"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPE65325.1"
                     /translation="MTSFCIVLSGRGSNMEALADAVRDGRLDARVEFVASDAPDAPGL
                     EKARRRGIDTVVLPYASEGKENAERRLSGLCSARGVEWIVLAGFMRILSPEFVTAHAG
                     RIVNIHPSLLPSFPGRDGIGDAWRFGVAVTGVTIHLVDAGIDSGRILAQRAVEIFPED
                     SLENLEEKIHTTEHALYWKTLSELFRTGVPAERRWRR"
BASE COUNT         1597 a         1967 c         2146 g         1560 t
ORIGIN      
        1 gcgcagacaa aaagccattc tgaagaacac gcctccaacc cggaaacgat gcctgccagc
       61 gggccgcttc tcttttcatc aacctccgaa gagctttttt cgacaggaag gcgatcctcg
      121 acggtacgaa tgccgtacag agagattata cccgacaaca cagtggaaac atacgaaact
      181 tggcgggggg cgactgaaag caggatgtcc ttcgaaaagg gaagaactct ccgaataacc
      241 ttcagcaaaa gcggctcgga gtcgacggcg agcgtaagct tttcaccgcc cattctcgaa
      301 ccctgccccc ctgcgagaat caatgttgtt cccggaaact cctcgataaa attactcaca
      361 aaatcacctt ccgggggaaa attgcggagc aatttactgt tgacattttt tcaatgtcga
      421 tgaataatgt aagtatatta atccttggag gtgccccacc ttggaaaaaa agaagttcct
      481 ttacgaaggc aaagcgaaaa aaatttatga tacggatgat gtgaatctct gtattataga
      541 gtacaaaaac tccctcacag catttaatgc cctgaaaaaa gataccatcg aaggcaaagg
      601 cgaacttaac aatctgatca gctcttctct cttcgagctc ctctccgaaa aaggcattcc
      661 cacgcacttc gtcaaaagag tcgacaacat tcaccagatc gtaaaaaaag tatccatcgt
      721 tccgatcgaa gtcgttgtcc gcaacattac caccggctcg ctgtgcaagc gccttggagt
      781 cgaggaaggg aaagtcttgc cccgtcccct tgtggaactc tatctgaagg acgacgccct
      841 cggagatccg cttatcaccg aagatcatgc gctgcttttt ggatggagca ccgaggaaca
      901 gcttttaaaa ataaaggaaa ttgctctgaa ggtcaatagc gttctttcgg agtacttcac
      961 gggattgcgt attatcctcg tcgatttcaa acttgagttc ggcgtcgatg ccgaaggccg
     1021 cttgcttctg gcagacgaag tctcgcctga tacctgccgt ttctgggaca gggacacgaa
     1081 gaaccgcctc gacaaagacc gtttccggaa ggacttggga gatgttcttg gagcgtatca
     1141 ggaaatatgg agaagaattt cctcctcgaa tgaggatggg gcgcgatgac ctaccgggtt
     1201 tccattttta tcgagctcca ggaaggtgtg cttgatatcc aggggaaagc ggttgaacgt
     1261 tctcttgccg gtatggggca cgacgcgttg cgttcggtga aggtcggtaa gtacattcag
     1321 gtgtgggtgg aaaccgggtc tgcggaggac gcacgaaaag aagccgagag aatgtgcgac
     1381 gacctgctgg taaacagtgt catcgagcag ttccgtatcg aagtggaggg ggagtaagtg
     1441 aacacggcgg tcatcgtctt tcccggcagc aactgcgacc aggatgtcat gcacgccgtc
     1501 gccgggagcc tcgggacaaa ggtccgtgcc gtctggcaca aagagacgac gcttcccgaa
     1561 gacaccgatt ttgttattct ccccgggggg ttctccttcg gcgactacct gcgctgcggc
     1621 gcgatggcgg ctcgttcccc tattatgaag gcggttgccg aacatgggcg ccggggaggt
     1681 ctgctgttgg gaatctgcaa cgggttccag attttgacag agtcacggct gcttcccggc
     1741 gcgctgcttc cgaaccgaac gctcaacttt gtctgccgcc cctgtttctt gcgtgtggag
     1801 agcgtatcga cgccgtttac ctcgctcttt tcgaaggggc aggtcgttca gttccctatt
     1861 gcccacggag acggactgta cacgctgcct gaagaggaac tgaaggctct tgaagatcgc
     1921 gggggcgttg tcttccgcta cagcgattcc gacgggctga ccggcgacga tgcgaatcca
     1981 aacgggtcgg cgaacaacat cgccgggatc gtcaatgagc ggggcaacat ccttggtctt
     2041 atgccgcacc ccgaaagggc gagcgaagcg cttaccggcg gagaggacgg ccgggttttc
     2101 tggcaatcta tcctcgaatc gtttacaaag ggaggccgat gaaatggagt acacacaagc
     2161 cggtcttcgc gagaccgaat acgaggagat ccgccgcgtc ctgggcaggg agccgaatga
     2221 atgcgagctc cgcatcatgg gggtcatgtg gtccgagcac tgcagctaca agtcgacgaa
     2281 gaacttgctg aagctctttc cgaacaacgg agatcgcgtg gttctcggac cgggcgaaaa
     2341 cgccggaatc atcgatcttg gagacgggct cggggcggcg ttcaaggcgg agagtcacaa
     2401 tcatccttca gcggttgccc cgtaccaggg agcagccacc ggtgtgggag gaattatccg
     2461 tgatattcta gcgctcggcg cacgaccggt cgcctcgatg gacggactct tcttcggaga
     2521 gccttcgtcc cccaggacgc agtcgcttgc atcgggtatc gttcacggtg tcggagggta
     2581 cgggaatgcc gtcggcgtgc ctactgtcgg cggaaagacg gcatatgatc cgtcctataa
     2641 cgaaaacccg ctgctgaacg cattctgcat cggactaatt ccgctggacg aaatcgtcag
     2701 ttcccgcacc gcgcgtccgg ggcaggcggt cgtcctgctc ggctcgaaga cgggaaggga
     2761 tgggatcgcc ggagctgcgt tcgcctcggt agagctctcc gaggataaca aggcgagcag
     2821 accatcgatc cagataggcg atccgttcgg agaaaagctc ctgatcgagg cgtgcctcga
     2881 aatgaatgag aaaaaactta tcgtgagtat gcaggacatg ggtgcggcgg ggattatctc
     2941 ctcttcaagc gaagtcgcgg cgaaaagcgg cgtcgggatg attttgcact ttgataagat
     3001 tcctctcagg gcggagaata tggaaccgtg ggagatcgct ctctccgaat cgcaggagcg
     3061 catgttgctg atcgttgacc gttcttatct gcatgaggtg atggaggttg cggaaaagtg
     3121 ggagctggac tgcgccgaat tcggcgagac agtcgaaggt gacgagtaca cgattctctt
     3181 ccacggggag agagccgctt cactccctgc gacgctcatc ggagaccggt gccctcttat
     3241 tccctggcct tcccgacgcc ctgcggatct tgaaaagaaa tgggaattcg acgtcgatga
     3301 tattcctctt cccgaagact ggaacgaaac gttgctctct cttctagcag atccttcgct
     3361 cgcttcgaag aaaaggatct acgagcagta cgattcgatg gtccagctga acactgttat
     3421 cggcccgggg catcctgtga gcatgctcag gattaagggg cgggattcgc tcattgcaat
     3481 gactcttgac gctgatccct ggaagtgttc gcttgatcct ttccgcggcg gggccgaaac
     3541 tgtcgcgaga accgtccgtg atctcgcagt cgccggcgca cgcgccgcag ggcttaccga
     3601 ctgtctgaac tttccttccc cggaggttcc ggagcagtac tgggtgctcg aagagtgcgt
     3661 caaggggatg gccgaggcgt gcggcgcctt tgactgtcct gtggtctcgg gaaatgtgag
     3721 tttatacaac gaaagccccg acggcgccat cctcccgact cccgtggttg gaacagtcgg
     3781 gattattccg tcgatcgatg ctgcgctttc ggccggtacg tgggaagagg gcgatgttct
     3841 cttcctcgtc ggcccgtgca atccatcgct cgccgggagt cgctaccttc tctcacaagg
     3901 aaagcttgca ggacgcccgc tccccttcta tccggaagct gaaaaagact ttgttgagag
     3961 agccctgaag actgcgtccg caaaagccgc acacagcggg cgcgcccttt cgggaggggg
     4021 gcttgcggca gcccttgcca aagagagcat ggagagcggt atcggcgctt ctatttctct
     4081 ttccatccct acgcgcaaag atattttgct cttcggcgaa ggctgcgcac gcgcgcttta
     4141 tgcggttcca atgagcaggg ttcctctttt taaagtgctt tggaacgggt acccttgcat
     4201 tcaactcgga cgtgtagggg gagagcgcct ctccgtcgag ggagcttttt cgcttactgt
     4261 ttcggagctt ctctcgaaat ggaggaatgg ttgaatgtgc ggcgtcttcg gcgcattctc
     4321 acccgatgga aaaaaagttc ttgaagacgt atacctcggg ctttacgcgc ttcagcacag
     4381 ggggcaggaa gccgcggggg ttgcgtggac gaacgctacg ggcggaatat cctcccgtaa
     4441 gggaaacgga cttgttcatc ttgcgctcga ccaggcggac cttgccgctg tggaatcttc
     4501 gtgcgcaatc gggcacgtcc gttactccac aaccggagga tcggagcttt caaacgcgca
     4561 acccctgacc gcgagcacat cgaagtgccc aatcgccgtg gcgcataacg gaaatctgac
     4621 caatgcgcag ggactcaagc tacttcttca aaaccgcggc gccatctttc attcgacgac
     4681 ggatacggaa gtgattctcc atctcatcgc ccatcaaccc cacaagcagc ctctggacgc
     4741 gctgctggat tctcttgtac gtctccgggg agcgtactct ctcgcgatga tcctgggaaa
     4801 ccggctggta gccgctcgcg acccttgggg ctttcgcccg ctggtactag gccgccggga
     4861 taacattcac tatgtttctt cggagacgtg cgcgctcaat cttgtgggag cgagcgtcgt
     4921 gcgggagatt gaacctggag aggttatcat aataggagaa gacggggtac attcgatacg
     4981 ccttcctgtc gagcccaaga gacgattcgg ctgcgcgttt gagtttgtct atttcgcgcg
     5041 tccggacagc gtgatcagcg gccgttcggt ctatgccgta cgtaaggaac tggggaaaaa
     5101 gcttgcgaaa agagcgcctt gccgggaagc ctctttagtt accggaatgc ccgacagcgg
     5161 aacgatcgct tctctcggct acgcggaaga gagcggcgtg ccctacgagc agagcgttgt
     5221 ccgcaaccgg tacgtgggac ggaccttcat cgaaccgaca agcagggtgc ggcatcttgg
     5281 tgtgcggatt aaactgaatc ccatcaccga ggtgctccaa gggaagaacg tcgtcgtcgt
     5341 cgacgactcg atagtgcgcg ggacgacatg cgaacggatg atctccatga tcaagtcgtg
     5401 cggcgcgaac caggtccatg tacgtatctc ctctcctccg gtgcgcttcc cttgctacta
     5461 tgggatcgac acccccacgc gggtggaact ggcagccgcg cgcatgaatc ttcaacagct
     5521 tgagcaggag gtcggcgccg attcgctggc gtacatcacc gaagaggatc ttctggaagc
     5581 tattggcctt cccgcgcaga gcgtctgcac agcctgtttt tcgggagatt acatggaagg
     5641 cggcgaagaa tatgaacctc agctataaaa acgcgggagt cgatatctcc ctcgcggata
     5701 agtgggtcga cgttatcaag ggaatttcca aatcaatgcc tgcaaagaag gagatcatcg
     5761 gaggcatagg agggttcagc ggtctgtaca gactccctgg aggaatggct ctagccggct
     5821 gttgcgacgg agtaggcaca aaaatcgaaa ttgccagggc cgcgggaatg tttacccata
     5881 tcgggcagga tcttgtcgcc atgaacgtca acgatctgat tacctgcgga gcccgtccgc
     5941 tcttctttct tgactacatc gcctgcggga aactgaatat ccccgttctt caaccgatcg
     6001 tcgaaagtgt cgccagggcg tgcggcgagt gcgggtgcgc actgcttggc ggtgaaaccg
     6061 ccgagatgcc gggcgtctac cccgaggatg gactcgacct cgccgggttt gcggtcggca
     6121 tcgtcgaaga agaaaagata atcgacggga aaaatgttgc ggcgggagac gccgtcgtcg
     6181 gtattcccag ctccggcgtt cacagcaacg gtttttccct ggttcgccgc gctctctttt
     6241 ctgaaggtac gccgtacagc cttgacgaag cgatgccgcg cttcggaggc gccacgcttg
     6301 cggaaattct ccttgctccg acaaagctct acgtcaacac agcccttcgg gccgtctcca
     6361 ctggaaaagt gaaggctatg gcgcacatta ccggcggcgg gcttgcagat aaccttcggc
     6421 gcgtcgttcc ggacggactc ggcctctccc tggactatac agcctggccg aggcccgcta
     6481 tttttaacct gctctccgaa gagggggtcg aagaggagga gatgcgccgc gtcttcaacc
     6541 tcggtattgg ctactgcctg atcatagcgc aagaagatgt gtcgctggtt ctagagcaga
     6601 ttgaagcgca gagagagcaa ggaatcatta tcggagaggt tgttgcatga cgtccttctg
     6661 catcgtcctc tcaggacggg ggagcaacat ggaggcgctt gccgacgcgg tgcgcgacgg
     6721 aaggcttgat gcgagggtcg aatttgttgc atccgacgcg cccgacgccc ccggacttga
     6781 gaaggcgcgc agacggggaa tcgacacggt tgttcttccg tacgcctcag aaggaaagga
     6841 aaacgcggag cgccgactgt ccgggctttg ctccgcccga ggcgtcgaat ggatcgttct
     6901 cgccgggttc atgcgaattc tgtctccgga gtttgttacg gcgcatgccg ggcgtatcgt
     6961 gaacatccat ccgtcgctcc tcccgtcctt ccccggaaga gacggcatcg gcgacgcgtg
     7021 gagattcgga gtcgccgtaa ccggcgtcac gattcacctt gtcgacgcag gtattgattc
     7081 cggccgtatc cttgcacaac gggcggtaga aattttcccc gaagacagcc ttgaaaacct
     7141 tgaagaaaaa atacatacga ccgaacatgc tctatactgg aaaacgcttt cggaactctt
     7201 ccgaacgggg gttccggctg aaaggagatg gcgacgatga aagtgcggcg cgcgcttctc
     7261 tccgtctatg
//
LOCUS       DAOPKU010000088         7265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_10911_length_7265_cov_5.212760, whole genome shotgun sequence.
ACCESSION   DAOPKU010000088 DAOPKU010000000
VERSION     DAOPKU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10911_length_7265_cov_5.212760"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PK849_04035"
     CDS             <1..791
                     /locus_tag="PK849_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE65326.1"
                     /translation="VRATSRSTSASRENCRLVASGRAHIGMTMGSALYQAITGTEAFE
                     PDGKLPLQILMSMYPAPQHLVTVKGSGITKLEDIKGKRVSVGAPGGGDQILTNLILAA
                     AGIDPEKDFTKQQLTQPEGVMALKDGNVDAVFWNFAAPGSAVLEVAAQRDVVMIPLEE
                     ALVQKIIEANPFLIPYTIKAGVYESIKEDIRTIADSNYLVVKNDMDEALSYNLVKTLI
                     ENKDKFMNITTQAAHFVAEEASVGITEFASGAKKYFKEQGIDMK"
     gene            892..2847
                     /locus_tag="PK849_04040"
     CDS             892..2847
                     /locus_tag="PK849_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HPE65327.1"
                     /translation="MTIENNPPMEQIPLQKEIIEGRKRELEGWQYWVVAGIALAASLF
                     HLYTAAFGLLSAMYQRGVHWMFMGSLIFLIYPATKNRPKNKIDFWDWILAGMVVVGCL
                     NILLNWEAIAMREGMPVASDIYLGIMMVILVIEAGRRAMGWPLPIVAIIATIYALFGP
                     YFPGVLGHGGFPLEELAPFQYLRTDGIFGVPLGVSASFIFLFVLFGAVLSVSGAGQFF
                     IDLSLALTGRSQGGPGKAAVVASGLMGTVSGSSVANAVTTGAFTIPLMKEAGYSATFS
                     GAVVAASSTGGQVMPPVMGAAAFIMAQFLGISYWDIVVAAALPATLYFISIIAMVHFR
                     AGRRGIKSLTEETAPKMGKVLKEGWHLLLPIITLVVFLAYGYSAVKAVFWSIIIMVGV
                     SWLGKAQYRMTPKRVLDALISGGMGAVEVAAACACSGLVIGVIGITGVGLAFSSFVIS
                     LSGGVLPLALFLTMIGSIILGMGVPTTAQYIITSTLAAPALFQMGVPLMSAHLFCLYF
                     GVLADVTPPVALATYAAAGIAKSNAIKTGFTALVTAMAGFLVPYMFVYNNHLLFQGNL
                     LQIAFSFGTALMAIIGLAAGVQGYYFSVLSIFERVLLLIVPFLLIEPNIITDVIGLVI
                     LVSVYLLQRRKAAKHAKEKTVSDAAQS"
     gene            2866..3573
                     /locus_tag="PK849_04045"
     CDS             2866..3573
                     /locus_tag="PK849_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPE65328.1"
                     /translation="MAHKLIKPVMSAEEAVTHIQPGSSLMVGGFNYAGVPYTLIDALV
                     AQGTDNLHLISNDTLYANDRHPEGIGHGKLVVNGQCRKVTASHVGLNRETQRLFNEGK
                     MELELVPQGSFVERIRCGGFGLGGFLTPTGVGTIHEEGKQVIEVDGKKFILELPLKAD
                     VAIIRGYKADRWGNLTYYGTGRNFNPAMATAADLVIAEVDMILAQGDIDPNDVVTPGM
                     LVDILVLKGDGYYASRT"
     gene            3557..4231
                     /locus_tag="PK849_04050"
     CDS             3557..4231
                     /locus_tag="PK849_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HPE65329.1"
                     /translation="MLPVLDEEVVRHRIAQRIALDFEDGAVVNLGIGIPTLVSDYLPE
                     GVHVMLQTENGVIGAGPKPEVDDLRLIGAGGRCLSLLPGSSIVASDMSFGLIRGGHLD
                     ATVLGALEVDSHSNLANWMIPGKMVPGMGGAMDLVTGARAVYVATTHCDKKGRPKLVK
                     ECTLPLTGANVVSVVVTEFCLIRKIDGKMVLCEIAPEVTPEELKANTTMEYDVAPDLA
                     PMKGIE"
     gene            4257..5438
                     /locus_tag="PK849_04055"
     CDS             4257..5438
                     /locus_tag="PK849_04055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPE65330.1"
                     /translation="MGKPVLLSACRTPGGKFGGALKDLAAPDLGAIALAEAVKRSGVS
                     PEDIGEVIMGNGWQAGIGANPARIAMFRAGLPQSIPAFTINKRCGSSLKTAMLAADKI
                     RLGDMVAVAAGGMESASNVPYILPEARWGYRMGEKKALDALHKDGFHCPLSDMLMGAT
                     AEVLVEERSITREEQDEYALNSHRKAVAAIEGGFFAEEIVPVPVKDRKKGTVMVDTDE
                     IPRSDTSMESLGRLPALFKKDGTITAGSSSALCDAGSAYIVADSDWAAANGFTPLAEI
                     LGYASGALDALHMGLGPVVAMPKALQHAGMSLEDMEIIEINEAFSAQILACHRDMPFD
                     MEKLNPCGGAIALGHPIGATGGKILATLIYSLKRQKKEFGIVSACIGGGQGVAMVLRN
                     L"
     gene            5709..5954
                     /locus_tag="PK849_04060"
     CDS             5709..5954
                     /locus_tag="PK849_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65331.1"
                     /translation="MSRAFIKENEDQESYLEWQKLLRDREELLRILEKKKKYLLEDPA
                     AGTIPEEKRHEMIAKYDEEAEEVRRLLDEMLAETKIP"
     gene            6113..7024
                     /locus_tag="PK849_04065"
     CDS             6113..7024
                     /locus_tag="PK849_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPE65332.1"
                     /translation="MDRREFLKRSLAAGVGGAALLVPGSVKRVFADSGKLPGLAAVRG
                     GEPGEMFDRGIAALGGMERFVKKGQTVAIKPNMSWDVAPELAANTNPDLVERIVARCL
                     EAGASKVYAFDHTCDLWRNCYANSGIEAAVKRAGGIVSPAESASSYTKVSIPGARVLK
                     ETSIHRLLLDCDVFINVPVLKHHGGAGITMGMKNLMGTVWNRGEFHSKGLHQCIADVS
                     LLRRPDLTVIDAYRMLTRNGPRGTSASDVTVLKAQILSTDIVAGDCAGARIFGLDPKN
                     VGHIRMGAELGAGTMDLDSLGVARIVL"
BASE COUNT         1463 a         1976 c         2308 g         1518 t
ORIGIN      
        1 gcgtacgggc gacatcgcgc tccacttccg cgtcgcgtga gaactgccgt cttgtcgcgt
       61 ccggacgtgc gcatatcggt atgaccatgg ggtccgcgct ctaccaggcg atcaccggca
      121 cggaggcttt cgagccggac ggcaagctcc ccctgcagat tctcatgagc atgtaccccg
      181 cgccgcagca cctggtaaca gtcaagggaa gcggcatcac caagcttgag gacatcaagg
      241 gcaagagagt gtccgtcggc gcgcccggcg gcggcgacca gattctgacc aacctcattc
      301 tcgcggcggc gggaatcgat cctgaaaagg attttacgaa acagcagctg actcagcctg
      361 aaggcgtcat ggcgctgaag gatggaaacg tggatgcggt cttctggaac ttcgcggccc
      421 ccggttcggc ggtgcttgaa gtggccgcac agcgcgacgt ggtgatgatt ccgcttgagg
      481 aggcgcttgt gcagaagatc atcgaggcga accccttcct gattccgtac acgatcaagg
      541 ccggcgtcta cgagagcatc aaggaggata tccggacgat cgccgacagc aactacctcg
      601 tggtgaagaa cgatatggac gaggcgctct catacaacct ggtgaagacg cttatcgaaa
      661 ataaggacaa gttcatgaac atcaccacgc aggccgccca cttcgtcgcc gaagaggcca
      721 gcgtcggtat tacggagttt gcctcggggg ccaagaagta tttcaaagaa cagggaatcg
      781 atatgaaata aaaaagaata gcacgcggca gcagtttttc tcccggccgg tcgttttcgg
      841 tcggccggga actttacagg ttctaatttc actccgtctg gaggtttcta tatgacgatc
      901 gaaaataatc cgcccatgga acagattcct ctgcagaaag aaataatcga aggaagaaag
      961 agggagctcg aagggtggca atactgggtg gtggcgggca tcgcgctcgc ggcgtcgctg
     1021 ttccacctgt acacggcggc tttcggcctc ctctctgcga tgtatcagcg tggcgtccac
     1081 tggatgttca tggggagcct tattttctta atctacccgg cgacaaaaaa tcgtccgaag
     1141 aacaagatcg atttctggga ctggatattg gctggtatgg tagtcgtcgg ctgcctgaac
     1201 atcctcctga actgggaggc gatcgccatg cgtgagggaa tgcccgtcgc ttccgatatc
     1261 tacctgggaa tcatgatggt gattctggtc atcgaagccg gccgccgcgc tatggggtgg
     1321 ccgctgccca tcgtggcgat catcgcgacg atctacgcgc tcttcggccc ctacttcccg
     1381 ggtgtgctcg gacacggagg ctttcccttg gaagagcttg ctcccttcca gtatctgcgc
     1441 accgacggta tcttcggggt gccgctcggc gtctcggctt cgttcatctt tctcttcgtt
     1501 ctcttcgggg ctgtcctgag cgtttccggc gcggggcagt tctttattga cctttcgctt
     1561 gccctcaccg gccggagcca gggagggcct ggcaaagcag ccgtcgtggc gagcggcctg
     1621 atgggaacgg tttccggaag ctccgttgca aatgccgtaa caacaggcgc cttcaccatt
     1681 ccgttgatga aggaggccgg atacagcgcg acgttctcgg gagccgtcgt ggcggcgtcc
     1741 tccacgggcg gtcaggtcat gccgccggtc atgggggctg cggcgtttat catggcgcag
     1801 tttctgggta tttcctactg ggatatcgtc gttgctgctg cgcttcccgc aacgctctat
     1861 ttcatctcca tcatcgcgat ggtccatttt cgtgccggaa ggcgagggat caagagtctc
     1921 acagaagaga ccgctcccaa gatggggaag gtgctgaagg aagggtggca tcttctgctg
     1981 ccgatcatca ccctcgtcgt ctttctcgca tacggctatt ccgcggtgaa agcggtcttc
     2041 tggtcgatca tcatcatggt cggcgtttcc tggctcggca aggcgcagta ccgcatgacg
     2101 ccgaagcgtg ttctcgacgc tctgatcagc ggcggcatgg gggcggtgga agttgcggcc
     2161 gcgtgcgcat gttcggggct tgtcatcggc gtcatcggta tcaccggagt cgggcttgcg
     2221 ttctcctcct ttgttatcag cctctccggc ggcgtgcttc cgctcgctct cttcctcacg
     2281 atgatcggct cgatcattct tggtatgggc gttcccacca cggcgcagta catcatcaca
     2341 tcgacgctgg ctgctcccgc gctttttcag atgggcgttc cgctcatgtc ggcgcacctc
     2401 ttctgtctct atttcggcgt gctcgcggat gtaacgccgc cggtagcgct ggcgacctac
     2461 gccgctgccg gaatcgcaaa gagcaacgcg atcaagaccg gattcaccgc gctggttacg
     2521 gcaatggcag gattcctcgt cccttacatg ttcgtttata ataaccacct gctcttccag
     2581 gggaacctgc tgcaaatcgc gttctccttc ggaacggcgc tcatggcaat catcggtctc
     2641 gcggcgggtg tccaggggta ctacttctcg gtcctctcaa ttttcgagag agtgcttctg
     2701 cttattgttc ccttcctgct catcgaaccg aatatcatca cagacgtgat cgggctcgtc
     2761 attcttgtga gcgtctatct actgcaaaga cgcaaagcgg cgaagcacgc gaaagaaaaa
     2821 actgtttcag acgctgcaca atcttaaagg aggtgtcatc tcttcatggc gcataagctc
     2881 atcaagcccg tcatgtccgc cgaggaggcc gtgacgcaca tacagcccgg ttcctcgctg
     2941 atggtgggag gattcaacta tgcgggggtt ccctataccc tgatcgacgc gctcgtggcg
     3001 caggggacgg acaacctgca tcttatttcg aacgatacgc tctacgccaa cgaccggcac
     3061 ccggaaggga taggccacgg caagcttgtt gtgaacggtc agtgcagaaa agtgaccgcg
     3121 tcgcacgtcg ggctgaaccg ggaaacgcag agactcttca acgagggaaa aatggagctc
     3181 gaactggttc cgcaggggtc tttcgtggaa cggatccgct gcggagggtt cggcctcggc
     3241 ggttttctga cgccgaccgg cgtcgggacg attcacgagg aggggaagca ggtcatcgag
     3301 gtcgacggaa aaaagtttat tctcgaactt cccctgaagg cggacgtggc gatcatccgg
     3361 gggtacaagg cggaccgctg gggaaacctg acctattacg gcacgggacg gaacttcaac
     3421 cccgcgatgg cgacggcggc cgatctggtg atcgcggagg tcgatatgat cctggctcag
     3481 ggagatatcg acccgaacga cgtcgtcacc cccggaatgc tcgtcgatat actcgttctg
     3541 aaaggagacg gatactatgc ttcccgtact tgatgaagaa gttgtccgcc atagaatagc
     3601 acaacggatc gcgctcgatt tcgaggacgg cgctgtggtg aacctcggca tcggcattcc
     3661 gacgctggtt tcagattacc ttcccgaggg tgtgcacgta atgctgcaga cggagaacgg
     3721 cgtgatcgga gccggtccta aaccggaggt ggacgacctt cggctcatcg gcgcgggggg
     3781 gcgctgcctc tcccttcttc ccggttcgtc catcgttgcc agtgatatga gcttcgggct
     3841 catcagggga ggtcatctcg acgcgacggt gctcggcgcc ctcgaggtgg acagtcattc
     3901 gaatctcgcc aactggatga tccccggcaa aatggtgccg ggcatgggcg gggcgatgga
     3961 tctggtgacc ggcgccagag cggtgtacgt cgccacgacg cactgcgaca agaaggggcg
     4021 cccgaagctc gtgaaggagt gcactttgcc gcttaccgga gcaaacgtgg tcagcgtcgt
     4081 cgtcaccgag ttttgtctta tacgtaagat cgacgggaag atggtgctct gtgaaattgc
     4141 gccggaagtc actcccgagg agctgaaggc gaacacaacg atggagtacg acgtcgctcc
     4201 ggatctcgcg cctatgaagg gcatcgagta gcgaatacga aaggacagga agaaaaatgg
     4261 gcaaaccggt actattaagc gcgtgcagaa cacccggcgg gaaattcggc ggggcgctca
     4321 aggatcttgc cgcccccgac ctgggagcga ttgctcttgc cgaggcggtg aagcggtcgg
     4381 gcgtatctcc ggaggatatc ggcgaggtca tcatgggaaa cggctggcag gcgggcatcg
     4441 gggcgaatcc ggcgcgtatc gcgatgttcc gcgccggact ccctcagtcc attcctgcgt
     4501 tcacgatcaa caagcgctgc ggctccagcc tcaagacggc gatgctcgct gcggacaaga
     4561 ttcgcctcgg ggacatggtc gccgtggctg cgggcggtat ggagagcgct tccaacgtgc
     4621 cgtacattct tccggaggcc cgatgggggt accggatggg tgagaagaag gcgctcgacg
     4681 cgctccacaa ggacggattc cactgccctc tttcggatat gctcatgggg gcgacggcgg
     4741 aggtgctcgt cgaggagcgc tccatcacca gggaagaaca ggacgaatac gcgctcaaca
     4801 gccacaggaa ggccgtcgcg gcaatagaag gcggtttctt cgccgaggag atcgttcccg
     4861 ttccggtgaa ggacaggaag aaggggaccg tcatggtcga taccgacgag attcccagaa
     4921 gcgacacgtc gatggaatcg ttggggaggc tgcccgcgct cttcaaaaag gacgggacga
     4981 ttacggcggg ttccagctcc gccctgtgcg acgccggaag cgcgtatata gtagccgaca
     5041 gcgattgggc tgcggcgaac ggcttcacac ctttggcgga gatactcgga tacgcttcgg
     5101 gcgcgctcga tgcccttcat atggggctcg ggcctgtcgt ggccatgccg aaagcgcttc
     5161 aacacgccgg aatgtcgctt gaggatatgg agatcatcga gatcaacgag gccttctccg
     5221 cgcagatcct cgcatgccac agggatatgc cgttcgacat ggagaagctg aatccctgcg
     5281 ggggcgcgat cgcgctcggt cacccgatag gggcgaccgg aggaaagatc ctcgcgacgc
     5341 tgatctactc gctgaaacgc caaaagaagg agttcgggat cgtcagcgcg tgtatcggcg
     5401 gcggccaggg agtggcgatg gtgcttcgga atctgtaagt acgccagagt tccggcactc
     5461 ttcgaagaaa gcgcgcgggc cgtttgtgta cgccggtccc gcgcgctttt tattccacgc
     5521 ttctcgatcg tcggtacgag cgggcgcttc ggatgcggag gcgcttgcaa aagtttcgcg
     5581 gttgggcgat aatagaatcg aaaacgcagt tttttctgtc gtcatcatga agagccaaca
     5641 gggccccgga tcctttccgt ggccttgtgt attgttgcgc ttttcatcgt ttgagagggg
     5701 ggatagctgt gagcagagcg tttattaaag agaacgagga tcaggagagc tacttggaat
     5761 ggcagaagct tcttcgggac agggaagagc tgctgcgtat ccttgagaag aaaaagaagt
     5821 atcttttgga agatcctgcc gccgggacaa tacccgaaga gaagcgccat gagatgatcg
     5881 cgaaatacga tgaagaagcc gaggaggttc ggcgtcttct ggatgagatg ctggcggaga
     5941 cgaagatacc ctgatttttc gcctgagagt cgctgcaaaa agccgtcgta agacagggat
     6001 atgcattctc cctgatttac gacggctttt tgcactctac tcaggaatgt cgaataaatg
     6061 tacatcgcga acgtgctatt atataacaag agctgttcga ggaggtgtga gaatggaccg
     6121 gagagagttt ttgaagagga gccttgctgc gggggtagga ggcgcggcgc tgctggttcc
     6181 gggaagcgtg aagagggtct ttgcggactc tggaaagctg cccggactgg cggccgttcg
     6241 cggaggagaa cccggggaga tgttcgaccg cggcatcgcg gcccttggag gaatggagcg
     6301 ttttgtgaag aaagggcaga ctgttgcgat caagccgaac atgtcctggg acgtcgcgcc
     6361 cgagctggcg gcgaacacga acccggatct cgtcgagcgc attgtggccc gctgcctgga
     6421 ggcgggagcg tcgaaggtgt atgcttttga tcatacgtgc gacctgtggc ggaactgtta
     6481 cgcgaacagc ggcatcgaag cggcggtgaa acgggccggc ggcatcgtct ccccggcgga
     6541 gtccgcgagc tcttacacga aggtttccat tccgggcgcg cgagtcctca aggagacctc
     6601 gattcaccgt ctgcttctcg actgcgacgt ttttatcaac gttcccgtgc ttaaacatca
     6661 cgggggcgcc gggatcacga tggggatgaa aaaccttatg ggaacggtat ggaacagggg
     6721 agagtttcac tcgaaggggc tgcatcagtg catagccgat gtgagccttc tccgcaggcc
     6781 ggaccttacg gtgatcgacg cgtacaggat gctcacgcgg aacgggccgc gcgggacctc
     6841 cgcgagcgac gtgaccgtac tgaaggcgca gatcctctcg acggacatcg tggccggaga
     6901 ctgcgccggg gcgaggattt tcggccttga tccgaaaaat gtcgggcata ttcgtatggg
     6961 cgcggagctc ggcgcgggga cgatggatct cgattcactc ggcgtagcgc gcatcgtatt
     7021 gtgatggaca ggaagagagc gggagcggcg cggaaactcc gctccctttt tgctctggcg
     7081 gttcttcttc tcatgacgat gttctttctc ttcttcgcgg gagtgagaga gggaggcctt
     7141 ccgtggttgc aggccgttcc gtcggtactt cgattgtgga ccggagccct tatccttgct
     7201 gcaagcgccg ccctggcggg gcgcgtgtac tgctccgtcc tctgtccgtt gggaacgctt
     7261 cagga
//
LOCUS       DAOPKU010000089         7211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11016_length_7211_cov_5.360537, whole genome shotgun sequence.
ACCESSION   DAOPKU010000089 DAOPKU010000000
VERSION     DAOPKU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11016_length_7211_cov_5.360537"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PK849_04070"
     CDS             complement(<1..381)
                     /locus_tag="PK849_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65333.1"
                     /translation="MKKAFYVLTVVIILALLSASGASAAEKSKEETLDAAYEHLVKVM
                     NPEEMKKLFALFDTMKDVGLSWNEDLLLPLRDIIACKTKEQLYVIWGMGSMNISYAML
                     FDKNPEGVWEGGREIDERIGFPRAE"
     gene            689..1492
                     /locus_tag="PK849_04075"
     CDS             689..1492
                     /locus_tag="PK849_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPE65334.1"
                     /translation="MMENSGIRVISRAFSLLVHLAQERRPLGISEIAEAVSLPKATVY
                     RILDSLESERAVMNRGGEYELGPVCLMLADAYRSQTGFAEAARPHLRALRDETKETVH
                     LFAYERGEFYYLDKLESPYQVRMHSRIGAKASLFRLSAGKALLARLSALEMNEIAEAV
                     PDEVAAEFPEILSRGFAVDNEQNERGLRCVGAAVFDRKGRPLGAVSVSAPVYRFPLEL
                     VESYGQLVKRTAEAIAGSADILFPAAAGGVARAGKSKGRLSSGTAPVEE"
     gene            1533..2690
                     /locus_tag="PK849_04080"
     CDS             1533..2690
                     /locus_tag="PK849_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPE65335.1"
                     /translation="MIKTNKLVMIPGPTPVVRSIQDQMARETVAFGDAAFVKDFSEVI
                     ADLKAMWRCDGEVFVVAGSGTMGMEMAIANTTKRGDNVLICSNGYFGDRFIDMCTRKG
                     LNTDVLAAEWGSSVTPESVEKALASKQYQVVTVTHVETSTGAEAPIAAIGEVMKKHPE
                     IIYIVDGVAASGGAEQYMDSMGIDVVLSCTQKAFGVAPGLTMVWASKKAMEKRATLGA
                     IPESYIDFDKWLPVMHDPSKYWGTPAINLVWALKESVRIMKEEGLEERYARHRRQAAV
                     FDAALEAIGFKVAAQKAFRAPTLSVYLYPEGSGIDDMKFRTVLAAEGAQTAGCLGGFA
                     GKGFRMGHMGNIDKHTLVSAIAAVERSCVKCGYSIELGKALGVLQKGLLEE"
     gene            complement(2782..4494)
                     /locus_tag="PK849_04085"
     CDS             complement(2782..4494)
                     /locus_tag="PK849_04085"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE65336.1"
                     /translation="MKILLCNVSKKYETAAHDYVGLFYLAGALEAAGFEPLVFHGKAE
                     GLLDEIARHRPEAVGFSCDFDNVRIIEDVSQKIKERWGIPVVVGGPQSIALGRTFLES
                     SGVDYILRGEGEISLPLLLNAVLKGEGELDEVKGLAMIKDGVFRETRPEIIDDPDALP
                     LPAYHTSLHSDRAYGNLIFTGRGCPFQCAYCAPSPGKRKVRLRNIPLVFEEIKTNLRN
                     NPGLRCLIVMDDTFTLGRERIEAFCEGMRELRKELDLVWYCECHVGRIKSFMDVLPVM
                     IDAGLIRLQIGIESGDQNVIDQYNKHVRIDDILEFVSYAADCGLAQIATNFIVGGPKE
                     REGATAELIRELIDRAPGVIDIITGFLRAYPGTEIFENPEKFGLQLHDESGRRTGDDY
                     PFVTPVGESQDQVMRRRQELNKVIRRSMQQKIKNRELKQETVMKQFEAALKYRIKSRW
                     FGEIADNPRAYEYYSLLYANEGEAFEESMDFSRDYPLRTFELWRAMTFSDGFPRLDGM
                     ALSPMEYELLLRCAGKRSLSTIQTELYELFGGSYEDEAEFRRHFVDMLRRCDDKYWLT
                     HFVI"
     gene            complement(4507..>7211)
                     /locus_tag="PK849_04090"
     CDS             complement(4507..>7211)
                     /locus_tag="PK849_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE65337.1"
                     /translation="MQIGIESCNQHIIDIYNKNVKREGFFEVIEICKAAKLPQLVGNI
                     IIGGALETRETLDYTFNTVYEMLRAGRGMVEVVSTFYMPFPETAMTKAPERFGVIVKD
                     EKALTSVGDFPVIETKALSIEEICAARNEFFEGTTGVMRKMFRDGGIPDETVMRSYAL
                     SERYGLSGMWQSLAYSKFPYLDAQYKARIRGDALTRDYDMHSMFDTYPQRVFLLSLTP
                     SLAADIPSVNGFVVSPFEFEVLKHSSGKLTMLEMAEAIFPDCRSAFENFEDFKPHLLE
                     TVKKLEAAGMCVLSGPLNRKAAERKWKKKKDAGEPRMKNKVILFKVFTIAMETAGFSR
                     NGAFLGLYGLVSYLDEQGYDAVACECRPDQVIEYIQRLPLDEVCCVGFSVDFENKHAV
                     EKISASIAETSGIPVLIGGPEAFSLDEEYLRGSKAAAIVRGEGELAMTAVLEALKAKK
                     SLDAIPGILYIDENGECVDRGEGPVVENLDELPFPAYEKSMTPVDYSNLYIMTSRGCP
                     YSCVFCHEGALKRPVRRRSVANVVAEMRHLLLKYPELKYFLFCDDTLVANPEWLDEFC
                     REVKALQAVRPFQFYCEADAASLSKRPEVIKDMVAAGLNRLQIGIESADREMLKIYRK
                     NLAPEMVETVVKAAYDAGVQQVFGALLVGGPFENREHIEKNKAFAARLMRLGPGMMEI
                     APSILMPYPMTDIGRHPEKYGLKIYDRQGVASISDYPLMESASMDRREIMAAYQEYIQ
                     SFVNEVKAMLDTGELGHEDILRAFRTSMNSKGPKYWSNIIAHHKPMLAPYYTMLAREA
                     ASRIGDVQPEELPKWRPQRLLEMWRDVDFSRGYPLARGEALSPFEYEVLKYSTGKSTI
                     AEIARILYERFGKPFGETTEDLTGRIVETLKAFDKKYWLLVVPY"
BASE COUNT         1419 a         2129 c         2042 g         1621 t
ORIGIN      
        1 ttccgccctg gggaagccga ttcgttcgtc gatttccctt ccgccttccc aaactccttc
       61 cggatttttg tcgaagagca tcgcgtagct gatgttcatt gaacccatac cccagataac
      121 atagagctgt tctttggttt tacaggcgat gatatcccgg agcggaagaa gaagatcctc
      181 gttccacgaa agaccgacgt ccttcatggt gtcgaagagc gcgaagagct tcttcatctc
      241 ttccggattc attaccttga ccagatgctc gtacgcggcg tcgagagtct cttccttgct
      301 cttttcggca gccgatgctc cggaggcact caggagcgcg aggatgatca caaccgtcag
      361 tacataaaaa gcttttttca taccagtctc tccttccctt gtctttcttt atgctgtccg
      421 aaattgttca ctacaccatg tgtgcttttc gccgtcctgt ttcccgctcc cttcattttt
      481 atcgtttccg gggattccgt tcctcgttga gaacgagtat gacatggaaa cccctcattt
      541 tcaaggaaac gccaaatcca acatattgca ggtaaagagt atgagagtat gcctcgtggc
      601 gcgcgtgcaa cgacggctct tgacattatg catactgcgt cctatgatga atctacacta
      661 cggataaata atccatggag cgaaatgaat gatggaaaac agcggcatca gagttatttc
      721 tcgtgcattt tcactccttg tgcatctggc gcaggagcgg cgccctctcg gcatctcgga
      781 gatcgcggag gcggtttcgc ttcccaaggc cacagtctac agaatcctgg acagccttga
      841 aagcgaacgc gcagtgatga accggggcgg cgagtacgaa cttggccccg tctgtctgat
      901 gctggccgac gcgtaccgca gtcagaccgg gttcgccgag gcggcgcgtc cgcacctccg
      961 cgcgttacgg gacgagacga aggagacggt ccacctcttc gcttacgagc ggggtgaatt
     1021 ttactacctt gataagctcg aaagccccta tcaggtgcgt atgcactccc gcatcggggc
     1081 gaaggcgtcg cttttccgtc tctcggcggg gaaggcgctt ctggcgcgcc tctctgccct
     1141 cgaaatgaac gagattgcag aggcggttcc ggacgaggtt gcggccgagt ttcccgagat
     1201 cctttcgagg gggttcgccg tggacaacga gcagaacgaa cgggggctgc gctgcgtcgg
     1261 cgcggcggtc ttcgacagga agggacgtcc gttgggagcg gtgagcgtct cggctcccgt
     1321 ctaccgtttt cccctggaac tggtcgaaag ctatggacaa cttgtgaagc ggacggccga
     1381 ggcgatcgcc gggagcgcgg atattctctt ccctgcggcc gccggcggcg ttgcgcgtgc
     1441 agggaagagt aaagggcgtc tatcgtccgg cacggcgccg gtcgaggaat gatctaccag
     1501 agaacgtttt tctacagaag gaggaaaaag agatgatcaa aacaaacaag ctcgtcatga
     1561 ttcccggtcc caccccggtg gtccgctcca tccaggacca gatggcccgc gagacggtcg
     1621 ccttcggcga cgccgcgttc gtcaaggact tctcggaggt catcgccgat ctgaaggcta
     1681 tgtggcgctg cgacggagag gtcttcgtcg ttgccggttc cggcacaatg gggatggaga
     1741 tggccatcgc gaacacgacg aagcgaggcg acaacgtcct catctgctcc aacggctact
     1801 tcggcgaccg cttcatcgat atgtgtaccc gcaaggggct gaacaccgat gttctcgccg
     1861 cggagtgggg atcttccgtc acgccggaat cggtggagaa ggcgcttgcc tcgaagcagt
     1921 accaggtggt caccgtgacg cacgtggaaa cctccacagg agcggaagct cccatcgccg
     1981 ccatcggcga ggtgatgaag aagcatcccg agatcatcta catcgtggac ggcgtggcgg
     2041 cctccggcgg cgcggagcag tacatggaca gcatggggat cgacgtcgtg ctctcctgca
     2101 cgcagaaggc gttcggcgtg gccccgggcc tcaccatggt ctgggcgagc aagaaggcga
     2161 tggagaagcg cgcgacgctc ggcgcgattc ccgagtcgta catcgacttc gacaagtggc
     2221 ttcccgtcat gcacgacccc tcgaagtact ggggcacccc cgccatcaac ctcgtctggg
     2281 cgctgaagga gtccgtgcgc atcatgaagg aggaggggct ggaggagcgg tatgcccggc
     2341 accgccgcca ggccgcggtg ttcgacgccg cgctggaggc catcggcttc aaagtcgccg
     2401 cgcagaaggc cttccgcgcg cctactcttt cggtctacct ctaccctgaa ggttcgggga
     2461 tcgacgacat gaagttccgc acagtcctcg ccgccgaggg cgcccagacc gcaggatgcc
     2521 tcggcggatt cgccggaaag ggtttccgca tggggcacat ggggaacatc gacaagcaca
     2581 cgctcgtctc ggccatcgcc gccgtggagc gctcatgcgt gaagtgcggc tattcgatcg
     2641 aactcggcaa ggccctcggc gtgttgcaga aagggctctt ggaggagtaa cagctccctt
     2701 tttacacgcg aataccccgt cttccggagc tctccggagg gcggggtatc aaccagcaac
     2761 gctccggact cccggtcgag gttagatcac gaagtgcgtc agccagtatt tatcgtcgca
     2821 ccggcgcagc atgtcgacga agtgacgacg gaattctgcc tcatcttcat acgagccgcc
     2881 gaagagttcg tagagttccg tctgaatcgt gctcagactt cttttgcccg cacaccgcag
     2941 gagaagttcg tactccatcg gcgagagcgc catgccgtca agccggggga aaccgtccga
     3001 aaacgtcatg gcgcgccata attcgaaggt cctcaggggg taatccctgg agaaatccat
     3061 ggactcttcg aacgcctctc cctcgtttgc ataaagcaag gagtagtatt cataggctcg
     3121 cgggttgtcc gcgatttccc cgaaccaccg gctctttatc ctgtatttca gcgccgcttc
     3181 aaactgtttc atcaccgttt cctgtttcag ttcacggttt ttgattttct gctgcatgct
     3241 gcggcggatc accttgttga gttcctggcg ccggcgcatc acctgatcct ggctctctcc
     3301 aaccggcgtc acgaaggggt agtcgtcccc cgtgcggcgg ccggattcgt cgtggagctg
     3361 gagcccgaat ttttccgggt tctcgaagat ctccgttccc ggataggccc gcagaaaacc
     3421 ggtgatgatg tcgatgacgc cgggcgcccg gtcgatcagc tcccggataa gctccgcagt
     3481 cgcgccttcc ctttccttcg gacctcccac aatgaagttc gtggcgatct gcgcgaggcc
     3541 gcagtcggcg gcgtacgaga cgaattccag aatgtcgtcg atgcgtacgt gtttattgta
     3601 ctggtcgatg acgttctgat caccggattc tattccgatc tgcagacgga tcagcccggc
     3661 gtcgatcatg acgggcagga catccataaa gcttttgatg cgccccacat ggcattcgca
     3721 gtaccagacc agatccagtt ctttccgcag ctcccgcatc ccttcacaga aggcttctat
     3781 gcgctccctg ccgagggtaa aggtgtcgtc catcacaatg aggcatcgca ggccgggatt
     3841 gttgcggagg tttgttttga tttcttcgaa caccaggggg atgttgcgca gcctgacctt
     3901 gcgtttgccg gggctggggg cgcagtaggc gcactggaat gggcaccctc tgccggtgaa
     3961 gatcaagttc ccgtaggcgc ggtcgctgtg cagcgaggtg tggtaggccg gcaggggcag
     4021 ggcgtccgga tcgtcgatga tctcgggccg tgtctcgcga aatacgccgt ccttgatcat
     4081 cgcgagcccc ttcacctcgt cgagttcgcc ctcgcccttc aagaccgcgt tgaggagcag
     4141 cggcagcgaa atttcgcctt caccccgcag gatatagtct acgccgctgc tttcaagaaa
     4201 cgtccttccc aaggcgatgc tctgggggcc gcccacaacg acgggaatcc cccagcgctc
     4261 ctttatcttt tgggagacgt cttcgatgat ccggacgttg tcgaaatcgc aggagaatcc
     4321 caccgcttcg gggcggtgtc tcgcgatctc atccaggagg ccttccgcct ttccgtgaaa
     4381 gaccagaggc tcgaatcctg ccgcctccaa ggcgcccgcc aggtaaaaga gccctacata
     4441 atcatgggcg gctgtttcgt attttttcga cacgttgcat agcagtatct tcatcacggc
     4501 gccttcttag tacggaacca caagcagcca gtatttttta tcgaacgcct tcaaggtctc
     4561 gacgatgcgc cccgtcagat cctccgtcgt ttctccgaag ggcttgccga atcgctcgta
     4621 gaggatgcgg gctatctcgg cgatggtgct cttacctgtg gagtatttca acacctcgta
     4681 ctcgaaaggc gacagagcct ccccccgcgc aagggggtag ccccgggaga agtccacgtc
     4741 gcgccacatt tcgagcaggc gttgtggacg ccacttgggc agttcctccg gctggacgtc
     4801 gccgatgcgg gaggccgctt cccgtgccag catggtgtag tagggggcca gcatgggttt
     4861 gtgatgagct atgatgttgc tccagtattt gggacctttt gaattcatgg aagtgcggaa
     4921 agctctcaga atatcctcgt gaccgagttc gccggtatcg agcatcgcct tcacctcgtt
     4981 cacaaaactc tgaatgtact cctgataggc cgccatgatt tcccgccggt ccatcgacgc
     5041 gctctccata aggggataat cggagatgga tgccacccct tgcctgtcat agatcttcag
     5101 tccgtatttt tcggggtggc gcccgatatc ggtcatcgga tagggcatga ggatactggg
     5161 ggcaatctcc atcatccccg gtcccaggcg catcagcctg gcggcaaagg ccttgttctt
     5221 ttcaatatgc tcacggttct caaaaggacc gcctaccaga agggccccga aaacctgctg
     5281 cactccggca tcgtaggcgg ctttcaccac cgtttccacc atttccggag cgaggttttt
     5341 tctgtatatc ttgagcatct cgcggtccgc ggattcgatg ccgatttgga gccgattgag
     5401 tccggccgcc accatgtcct ttatgacctc gggtcttttg ctcagggacg ccgcatccgc
     5461 ctcgcaatag aactggaagg gcctgacggc ctgaagcgcc tttacctccc ggcagaattc
     5521 atccagccat tcgggattgg cgaccagcgt atcgtcgcag aataaaaagt acttgagttc
     5581 agggtacttg aggaggagat gacgcatttc cgcaacgacg ttcgcgacgc tccgccggcg
     5641 cacggggcgt ttcaaggcgc cttcatgaca aaaaacgcag ctgtaggggc acccccggct
     5701 ggtcatgatg tacaggttgg agtaatcgac gggcgtcatg cttttctcat aggcggggaa
     5761 gggcagctcg tccagatttt ccaccaccgg cccttcgccc ctgtcgacgc actcgccgtt
     5821 ttcgtcgata tacaagatgc caggaatcgc atccaggctc ttctttgctt tcaaggcctc
     5881 caaaaccgcc gtcatggcca gttcgccttc gccccgtacg atggcggcgg ctttggaacc
     5941 gcggagatac tcttcatcca atgaaaaggc ttcggggcct ccgatcagca ccggaatccc
     6001 ggaggtttcc gctatggatg cactgatttt ttcgaccgca tgcttgttct cgaagtctac
     6061 cgaaaaaccg acgcagcata cttcatcgag cggaaggcgc tgaatatact cgatgacctg
     6121 gtccggacga cattcgcagg caacggcgtc atacccttgt tcatccagat aacttaccaa
     6181 cccgtagagt ccaagaaaag ctccgtttcg gctgaagccg gctgtctcca tggcaatggt
     6241 gaatactttg aacagaatga ccttgttttt catccgaggc tcccctgcat cttttttctt
     6301 tttccatttt cgttccgccg ctttccggtt caacgggccc gaaagaacgc acatccccgc
     6361 cgcttccagt tttttgacgg tctccagcag atgcggcttg aaatcttcaa aattctcgaa
     6421 ggcgctccga cagtcgggaa aaatcgcttc cgccatttcg agcatggtca gcttaccgga
     6481 ggagtgtttc aatacttcaa attcaaaggg tgaaacgacg aatccgttca cggacggtat
     6541 gtccgccgcc agagagggcg tcagcgaaag caggaaaacc cgctgcgggt acgtgtcgaa
     6601 catggagtgc atgtcatagt cccttgtgag cgcgtccccc cgaatcctgg ccttgtactg
     6661 cgcgtcgagg tagggaaatt tggagtacgc caggctctgc cacatcccgc tgagcccgta
     6721 gcgttcgctc agcgcgtagc ttcgcatcac cgtttcgtcg gggatcccgc cgtcgcggaa
     6781 catctttcgc atgactccgg tcgttccctc gaaaaactcg ttcctggccg cgcagatctc
     6841 ttcgatgctc agggctttcg tctcgatcac cggaaaatcc cccaccgagg taagcgcctt
     6901 ttcgtctttt acaataacgc cgaacctttc gggggctttc gtcatcgccg tttcgggaaa
     6961 tggcatgtag aaggtgctga ccacttccac catcccgcgg cctgcgcgca gcatttcata
     7021 gacggtgttg aacgtatagt ccagcgtctc ccgcgtttcc agagccccgc cgatgatgat
     7081 gttgcccaca agctggggaa gtttggccgc tttgcagatc tcgatgactt cgaagaaccc
     7141 ttcccgtttc acgtttttgt tatagatgtc gatgatgtgc tggttgcagc tttcaatgcc
     7201 gatctgcatc c
//
LOCUS       DAOPKU010000090         7196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11044_length_7196_cov_4.458479, whole genome shotgun sequence.
ACCESSION   DAOPKU010000090 DAOPKU010000000
VERSION     DAOPKU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7196
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11044_length_7196_cov_4.458479"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK849_04095"
     CDS             <1..193
                     /locus_tag="PK849_04095"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPE65338.1"
                     /translation="GDSVTLLGNVAVLTDEQTRKELWQEWFIKHFPKGPDDPDYCVLK
                     FDTVEATVYINEVFETHRV"
     gene            205..753
                     /locus_tag="PK849_04100"
     CDS             205..753
                     /locus_tag="PK849_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPE65339.1"
                     /translation="MFRPVLILGDPLLRRKSAPVDDFSDPALKDEMRDLKEALERFRR
                     EHGFGRGIAAPQIGIPKRMIALNLGQGTFVIANPEIVGRSDEMFTMWDDCMSFPDLLV
                     RVRRHVSVDLRYMDEDGAAKEWTGIGQAESELLQHEIDHLDGVMATDRAIEPEDILYK
                     SEYQKHKKLYDERVDYVIAPTI"
     gene            900..1097
                     /locus_tag="PK849_04105"
     CDS             900..1097
                     /locus_tag="PK849_04105"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HPE65340.1"
                     /translation="MRTNIVIDDELMEEAFTVSGGVSTKKELVELALREFVDTRKRRD
                     LRELRGRVAFADEYDYKSMRQ"
     gene            1100..1507
                     /locus_tag="PK849_04110"
     CDS             1100..1507
                     /locus_tag="PK849_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HPE65341.1"
                     /translation="MILVDTSVLVGYLKGSAGAVFDRFDEMIAGGVPFGINGYIYQEV
                     LQGARDEREYALLAEYLSSLPFYSLLHGRASFESAARIFFLCRRSGVTVRSTVDLLIA
                     QTAIENELYLFHNDRDFSNMAKIAPELKLIDYF"
     gene            1582..2226
                     /locus_tag="PK849_04115"
     CDS             1582..2226
                     /locus_tag="PK849_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HPE65342.1"
                     /translation="MEEIRMETWDRREHFEFFLRNDLPFYNVNFNADITGLREYAKGA
                     SVSLSAALIYLVTKSLNRVPNFLYRVLGGKVVRYERLHPQFTCLRGDEELFRLITVEF
                     RDDLAEFDRSVKRAIEESDAYFNVGMLAHRSDFVFISSLPWIPFTGIDHTLSLNKEDA
                     IPRVSWGRFFEQGGRTLLPCNIRVNHIFIDGLHVGRFYETFQEETRRLADSSAR"
     gene            complement(2256..2654)
                     /locus_tag="PK849_04120"
     CDS             complement(2256..2654)
                     /locus_tag="PK849_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPE65343.1"
                     /translation="MIVVVDASAAVEISLAKPSANRFREELLDAALTLAPDTYASEIT
                     NVFWKYRAYSGLPEQACLEGIEFCLGIVDEYIPSVELWREAFFEASKAKHPVYDMLYL
                     IVARRHNARLLTKDGKLAELASQSGIFVVS"
     gene            complement(2651..2896)
                     /locus_tag="PK849_04125"
     CDS             complement(2651..2896)
                     /locus_tag="PK849_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65344.1"
                     /translation="MPTLQVRDLPEELYQKMLYLAEKEHRSLTQTTIVLLKEGLERRL
                     ADTERRRDLLRNFSGIGPDAAGLPDAEILVREDRDRR"
     gene            3141..4499
                     /locus_tag="PK849_04130"
     CDS             3141..4499
                     /locus_tag="PK849_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPE65345.1"
                     /translation="MEKENTQYLKMIGMPVGKLILSLSVPTIVSMLVTAIYNIGDTYF
                     VSQLGTSASGAVGIVFSLMAIIQAVGFTVGMGSGAQISRLLGAKKVDEANTVAASGLF
                     AAIGLGSLVALFGLLFLDDLMRVLGATETILPYARDYASYILYAAPVMASSFLLNNIL
                     RAEGKAKFAMVGLTTGGLLNLALDPLFIFGFGWGIAGAAIATAISQCVSFGILFSWFL
                     RGKTIVRLSAASISRSPLEYLRILKNGFPSFCRQALASIATIALNVNAAVYGDAAVAA
                     MSIVGRLFMFVFVVNLGIGQGYMPVAGYNYGAGRFDRVKAAFLFTLKVTSAMMTLSGI
                     AAFMAAPSLIRIFIKTDPDVVRIGALALRAMCFAMPLIPLGVVCNMTFQSIGKSWTAT
                     VLSLARQGIFFLPLILVLPRFIGLTGVQITQPLADALTFLCCLPLAFFFFKELDAMSK
                     TN"
     gene            complement(4602..>7196)
                     /locus_tag="PK849_04135"
     CDS             complement(4602..>7196)
                     /locus_tag="PK849_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPE65346.1"
                     /translation="YALTGDGYTFRELEDHADFLRRELLLVEGVANVEISGVQRETIF
                     VEIDRSRMAQLGIGTQEIFNTLQAQNVVAGAGSVEVGPEYIRIAPSGYFSSVDELSNL
                     LLRGGDGRSIRLGDFATVTRGYADPPSSAMRYNGKPALGLGISNVDGGNVIRIGEAVH
                     KRIMELVPRTPVGMELGLIYYQPDTVADSIRNFVVNLLEALAIVIGILLVFMGFRSGF
                     LIGFILLLTILATFIVMKIMAIDLHSISLGALIIALGMLVDNAIVVADGILVRMQEGK
                     DGRAAAVEVVKQTQIPLLGATVIAAIAFAPIGLSPDSTGEFCKSLFQVVSISLLLSWV
                     LAVTVTPLAAVSILKGNAAGSEVDPYDTRLYRAYRGFLSFCLARRKFVLLVVAGLMVA
                     SFAAFGFVDKSFFPASTSPMFTVDFWTPRGTSVQATLAETRTMERYLLSLPETESVAA
                     YAGEGALRFILTYTPGDPASNYGHLIVTAKDSEAAEALKGKLDAFVRANQPHLDPRIR
                     SFAKGTGGGAKVQVRFLGKDPLALRRMAEQATRVFASDPDAVNIRNNWGERTKVIRPE
                     LNESVQLLGLTRRDVANALKMAFTGVSAGLYREGEKLLPIVARLPQSERGSVGSLEET
                     QIWSGLNRRYIPLSQFVSRVDTVAEDPYVYRINREKALTVECDSGSERPGALFARLRP
                     HIEALELPPGTEMEWGGEYENSQKAQGGLMGMIPLGFLGIVVILVMLFNGYRQPLIIL
                     LCLPLSVMGLSVGLLVMGKSFDFMALLGFLSLTGMLIKNAIVLIDQIDLEMREGKAPL
                     DAIIDSGVSRTRPVMMAALTTVLGMVPLYFDVLFSAMAVTIMFGLTFATALILVVVPV
                     LYGEALKA"
BASE COUNT         1336 a         2072 c         2362 g         1426 t
ORIGIN      
        1 tggcgacagc gtgacgctgc tggggaacgt cgccgtgctg acggacgagc agacgaggaa
       61 agagctgtgg caggagtggt tcatcaagca cttcccgaag gggccggacg acccggacta
      121 ctgcgttttg aagttcgaca ccgttgaggc gaccgtctat atcaacgaag tgttcgagac
      181 gcaccgcgta taggaggcgg aaaaatgttc agacccgtgc tgattctcgg cgaccctctt
      241 ctgcggcgga agtccgcgcc ggtagacgac ttttccgatc ccgcgctgaa ggacgagatg
      301 cgcgacctca aggaggcgct tgagcgattt cgccgcgagc acggcttcgg acggggcatc
      361 gccgcgccgc agatcggcat tccgaagcgg atgatcgcgc tgaacctcgg acagggaacg
      421 ttcgtgatcg cgaaccccga gatcgtcggc cgaagcgacg agatgttcac catgtgggac
      481 gactgcatgt ccttccccga tctgctggtg cgggtgaggc ggcacgtgtc ggtcgacctt
      541 cgctacatgg acgaggacgg cgccgcgaag gagtggacgg gcatcggcca ggccgagtcg
      601 gagctgctgc agcacgagat cgaccacctg gacggcgtca tggcgaccga cagggccatc
      661 gagccggagg acattctcta caagtcggag taccagaagc acaagaagct ctacgacgag
      721 cgtgtcgact acgtcatcgc gccgacgatc tgaacccgga gaagcgcgct gataggtggg
      781 agcgcgatct actctctttg cagcatgtgg aataatcgac gcgccctgaa caaccggaca
      841 gttccgtcct gattgctcgg ggcgttcgtg tgtattataa tgtgtatgag gtgatgtgta
      901 tgcgcacgaa tatcgtcatc gacgacgagc tgatggaaga ggccttcact gtttccggcg
      961 gcgtttcgac gaaaaaggag cttgtggaac tcgccctgag ggagttcgtg gacacgagaa
     1021 agagaaggga tctccgcgag cttcgggggc gggtcgcctt tgcggacgag tacgactaca
     1081 agtccatgag gcagtgagta tgatcctggt ggatacgtct gtccttgtgg ggtacctcaa
     1141 agggagcgcg ggcgccgttt tcgacaggtt cgacgagatg atcgccggcg gcgttccgtt
     1201 tggaatcaac gggtacatct atcaggaggt gcttcagggg gcgagggatg aaagagagta
     1261 tgctctcctt gccgaatatc tgtcttcttt gcctttctat tcgctgcttc acggtcgggc
     1321 gtcattcgag tccgcagccc gtattttctt tctgtgcagg cgttccggcg tcaccgtacg
     1381 cagcaccgtc gatttgctga tcgctcagac cgccatcgaa aacgaactgt atctctttca
     1441 caacgacaga gatttttcaa acatggcgaa gattgccccc gaactcaagc tgatagacta
     1501 tttttagcgc ttcgacggaa ttccgtcgaa gttttttgac gtgtcgttct tacgtgtcgg
     1561 gggcgtccgg aggaggtttg catggaagag attcgtatgg agacgtggga cagacgagag
     1621 catttcgagt tcttcctgag gaacgatctc cccttttaca acgtgaactt caacgcggat
     1681 atcaccggcc tgagggagta tgcgaagggg gcttccgtct cgctgagcgc tgcgttgatc
     1741 tacctggtga cgaagtcgct gaaccgtgtg ccgaacttcc tctacagggt gctcggcggc
     1801 aaggttgtgc ggtacgagcg cctgcaccct cagttcacct gtcttcgggg cgacgaagag
     1861 ctgttccggc tgatcacggt ggagttccgc gacgacctgg ccgaattcga ccggagcgtc
     1921 aagcgcgcga tcgaggagag cgacgcctat ttcaacgtgg ggatgctggc gcacaggagc
     1981 gacttcgtct tcatctcctc cctgccgtgg ataccgttta cggggatcga ccacacgctg
     2041 agcctgaaca aggaggacgc gattccccgc gtgtcgtggg ggcggttctt cgagcagggc
     2101 gggcggacgc tgctgccgtg caatatccgg gtgaatcata tcttcatcga cgggcttcat
     2161 gtggggcgtt tctacgagac gtttcaggaa gaaacgcgca gactggcgga ttcgtcggcc
     2221 cgctgaggcc ggtttccctt gcgggagtcg gctgtttacg aaacgacgaa gattccgctt
     2281 tgagaggcga gttcggcgag ctttccgtcc ttcgtgagca ggcgggcgtt atgtcgccgc
     2341 gcaacgatga gatagagcat gtcgtacacg gggtgcttcg ccttcgatgc ctcgaaaaac
     2401 gcctcccgcc agagctcaac cgaggggatg tactcgtcga caatgccgag gcagaattcg
     2461 attccctcca gacacgcctg ctcgggaagc ccggagtatg cgcggtactt ccagaagacg
     2521 ttcgttattt cggaggcgta ggtatccggc gcgagcgtca gcgcggcgtc gagaagttcc
     2581 tcgcggaacc ggttcgccga aggttttgcg agggagatct ccactgcggc gcttgcgtcg
     2641 acgacgacga tcatcgacgg tccctgtcct ctcggacgag aatctccgca tccggcagac
     2701 ctgcggcgtc ggggccgatt ccggaaaagt tccggagcag atcgcggcgg cgctccgtgt
     2761 cggcaaggcg ccgttcgagc ccctccttca gcagaacgat cgtcgtttgc gtaaggcttc
     2821 ggtgctcctt ctccgcaagg tagagcattt tttgatacag ctcttcgggt aaatcccgaa
     2881 cctgaagcgt cggcacgact cccacctccc tttcatgcat tctagatgtt ttttgcatgc
     2941 gaatcaagga ctcctcggag cgcgcattgc aacatggcgt gtttccgaag gattctaagc
     3001 gcacgtcttc aagcgatatt tcaagtgtac cccgagggaa tgaatacaga aaaagtcacc
     3061 gtgagtcgat acaataggcg gtgggtgagc tcatccccgc agatttttcg cacagcgcaa
     3121 aagaaagaag agttcgatca atggaaaaag aaaacacgca gtatctcaag atgataggga
     3181 tgccggtcgg caagctgatt ctgtcgctgt cggttccgac gattgtcagc atgctggtga
     3241 cggcgatcta caacataggg gatacctact ttgtctcgca gctcgggaca agcgcttcgg
     3301 gcgcggtggg catcgtcttt tcgctgatgg cgatcatcca ggcggtgggc ttcaccgtgg
     3361 gcatgggttc cggcgcgcag atctcgcggc tgctgggcgc caagaaggta gacgaagcga
     3421 atacggtggc ggcaagcggt ctctttgcgg cgatcgggct cgggtcgctg gtcgcgctct
     3481 tcggtttgct ctttctcgac gatctgatgc gcgttctggg cgcgacggag acgattttgc
     3541 cgtacgcccg ggactacgcg agctatattc tgtacgccgc gcccgttatg gcgtcgtcct
     3601 ttctgctgaa caacatcctc cgggcggaag ggaaggcgaa gttcgccatg gtcgggctga
     3661 cgaccggcgg tctgctcaac ctggcgctcg accccctgtt catcttcggc ttcgggtggg
     3721 gtatcgccgg ggccgctata gcgacggcga tcagccagtg cgtcagcttc ggcatcctgt
     3781 tttcctggtt ccttcggggg aagaccatcg tacgtttgag cgcggcgagt atctcgcgca
     3841 gtccgttgga gtaccttcgt atactcaaga acggcttccc ctccttctgc aggcaggcgc
     3901 tcgccagcat cgcgaccatc gcgctcaacg tgaacgccgc cgtgtacgga gacgccgccg
     3961 tggccgcgat gtccatcgtc ggcaggctct tcatgttcgt cttcgtggtc aatctgggga
     4021 tcgggcaggg gtacatgccc gtggcggggt acaactacgg cgccggacga ttcgacaggg
     4081 tgaaggctgc gtttctgttc acgctgaaag tgaccagcgc catgatgacg ctctccggaa
     4141 tcgctgcgtt catggcggcg ccgtccctga tcaggatctt tatcaagacc gaccccgacg
     4201 tggtgcggat cggcgccctg gcgttgcgtg cgatgtgttt cgccatgccg ctgattccgc
     4261 tcggcgtggt ctgcaacatg acctttcagt ccatcgggaa gtcgtggacg gcgacggtgc
     4321 tgtcactcgc ccggcagggc atcttctttt tgccgctgat cctcgttctt ccccgcttca
     4381 tcgggctgac cggcgttcag attacgcagc cgcttgccga tgcgctgacc ttcctctgct
     4441 gtctgccgct cgcgttcttt ttcttcaagg agctcgacgc catgtcgaag acgaactgaa
     4501 taccggcgca tcggagcgac attgtccggg aagacgcaac gcggcggttc gctgaaaagg
     4561 tgcgaaccgc cgcgttgttg tttccataag tatgcctgcc gctacgcctt cagcgcttct
     4621 ccgtagagca ccgggacgac cacgaggatg agcgccgtgg cgaaggtgag gccgaacatg
     4681 atggtgacgg ccatcgccga gaagaggacg tcgaagtaga gcgggaccat ccccaacacg
     4741 gtggtgagcg cggccatcat gacggggcgg gtgcggctga cgcccgagtc gatgatggcg
     4801 tcgagcgggg ccttcccttc ccgcatctcc aggtcgatct ggtcgatcag gacgatggcg
     4861 tttttgatga gcattccggt gaggctcagg aagcccagca gggccatgaa gtcgaacgac
     4921 tttcccatca cgagcaaccc caccgagagc cccatgaccg agagcggcag acagagcagg
     4981 atgataagcg gctgtcggta gccgttgaag agcatgacga gaatgaccac gatgccgagg
     5041 aagccgagag ggatcatccc catcaggccg ccctgcgcct tctgcgaatt ctcgtactcg
     5101 ccgccccact ccatctccgt ccccggcggc agctcgagcg cctcgatgtg cgggcgcagt
     5161 cgtgcaaaga gcgctcccgg ccgctcggag ccgctgtcgc actccacggt gagcgctttt
     5221 tcccggttga tccggtagac gtacggatcc tccgccacgg tgtcgacgcg cgagacgaac
     5281 tgcgacagcg ggatgtaccg tcggttgagg ccgctccata tctgcgtctc ttcgagcgac
     5341 cccacgctcc cccgttcgga ctgcggaagg cgcgccacga taggcaatag cttttccccc
     5401 tcgcggtaga gccccgcgct cactccggtg aaggccatct tgagcgcgtt cgccacgtcg
     5461 cggcgggtta gtccgaggag ctggacggat tcgttcagct cggggcggat aaccttcgtg
     5521 cgttcccccc agttgttgcg gatgttcacc gcgtccgggt cggaggcgaa gacgcgcgtc
     5581 gcctgttcgg ccattcgccg cagcgcgagc gggtccttcc ccaggaagcg cacctgtacc
     5641 ttcgcgccgc ccccggttcc cttggcgaac gagcggatgc gcgggtcgag gtggggctgg
     5701 ttggcccgga cgaaggcgtc gagcttgccc ttgagggctt ccgccgcctc gctgtccttc
     5761 gcggtgacga tcaggtggcc gtagttggag gccgggtcgc cgggcgtata ggtgaggatg
     5821 aagcggagcg ctccctcccc ggcgtatgcg gcgacggact cggtttccgg cagggagagc
     5881 aggtagcgct ccatcgtccg cgtctccgcg agcgtggcct gcacgctggt tccgcgcggg
     5941 gtccagaagt ccacagtgaa catggggctt gtggaggcgg ggaagaagga tttgtccacg
     6001 aagccgaagg cggcgaagga ggcgaccatc aaccccgcaa ccaccagaag cacgaatttg
     6061 cgccgggcga ggcagaagga gaggaagccc cggtaggcgc ggtagaggcg agtgtcgtac
     6121 gggtcgactt ccgagcccgc tgcgttcccc ttgaggatgg aaacggctgc gagcggggtg
     6181 acggtgacgg cgagtaccca gctcaacagc agcgagatgc tgacaacctg gaagaggctc
     6241 ttgcagaact cccccgtact gtccggcgag aggccgatgg gggcgaaggc gatggcggcg
     6301 atgactgtcg cgcccagcag cgggatctgc gtctgcttga ccacttcgac ggcggcggct
     6361 ctgccgtcct tgccctcctg catccggacg aggatgccgt ccgccacgac gatggcgttg
     6421 tccaccagca tccccagcgc gatgatgagc gcgccgagcg agatgctgtg gaggtcgatg
     6481 gccatgatct tcatcacgat gaaggtggcg aggatggtga gcagcaggat gaaaccgatc
     6541 aggaagccgc tgcggaagcc catgaagacc agcagaatgc cgatgacgat ggcgagggcc
     6601 tccagcaggt tgacgacgaa gttgcggatg gagtcggcga cggtgtccgg ctggtagtag
     6661 atcaggccga gctccatgcc gacgggcgtg cgggggacga gctccatgat gcgcttgtgg
     6721 acggcctcgc cgatgcggat gacgttgccc ccgtcgacgt tggagatgcc cagaccgagc
     6781 gcgggcttgc cgttgtagcg catggccgag gacggcgggt ctgcgtaccc ccgagtcacc
     6841 gtcgcgaagt cgccgaggcg gatggagcgc ccgtcgcctc cgcggagcag cagattcgag
     6901 agctcgtcca ccgaagaaaa gtagcccgac ggggcgatgc ggatgtactc cggccccacc
     6961 tcgacagagc ccgcgcccgc gacgacgttc tgcgcctgga gagtgttgaa gatctcctgc
     7021 gtgccgatgc cgagctgcgc catgcgcgag cggtcgattt cgacgaagat ggtttcgcgc
     7081 tggacgccgg agatctctac gttggcgacc ccttcaacga ggagcagttc gcgccggagg
     7141 aagtcggcgt ggtcctcgag ttcgcggaag gtgtagccgt cgcccgtaag ggcgta
//
LOCUS       DAOPKU010000091         7178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11076_length_7178_cov_4.278394, whole genome shotgun sequence.
ACCESSION   DAOPKU010000091 DAOPKU010000000
VERSION     DAOPKU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11076_length_7178_cov_4.278394"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PK849_04140"
     CDS             complement(<1..643)
                     /locus_tag="PK849_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65347.1"
                     /translation="MPDVAFWQGNEALAHGALAAGCRFFAGYPITPSTEIAEIMAEEL
                     PKLNGRFIQMEDEIAGIAATIGGSIAGRKALTATSGPGFSLKQENLGFAYIAEIPIVV
                     VDVMRGGPSTGLPTRTAQQDVMQARWGTHGDHGTICYAPSSVQECYEIAIKAFNAAER
                     YRHPRQPKRGRIRHPAVLVDAAIADQLEVLCRMTRRGVRALECVQKAGAIERLL"
     gene            complement(636..848)
                     /locus_tag="PK849_04145"
     CDS             complement(636..848)
                     /locus_tag="PK849_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE65348.1"
                     /translation="MPKKFEVLVNNSWCKGCSLCINICPKKVLELDEVRLKSVAARPE
                     DCIGCRQCENICPDLAITVKEDTKDA"
     gene            complement(1011..1688)
                     /gene="tsaB"
                     /locus_tag="PK849_04150"
     CDS             complement(1011..1688)
                     /gene="tsaB"
                     /locus_tag="PK849_04150"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPE65349.1"
                     /translation="MKEYTLSVDCCSAWTTLGLAEDEKVRGEINIDAGKNQSALLPGL
                     LQHFLKAFSLTPEDISLFSVVTGPGSFTGIKVGISFVTFLAWAAGKSIVPLSSLECMA
                     FEKIRRSGGLAASVLWGGGGKVYGGLFKGEGDTLPPLSLFRSGSFTPELFLEAFSGTK
                     LRHQDVFWLTDAPEKVAPLFPSFGGSFEKIIPTGSATVELTRRHKGRARSAFEIHADY
                     FRDPDLG"
     gene            complement(1688..2191)
                     /gene="tsaE"
                     /locus_tag="PK849_04155"
     CDS             complement(1688..2191)
                     /gene="tsaE"
                     /locus_tag="PK849_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPE65350.1"
                     /translation="MDSISCCEYISFSEESTMQIGELMAEHSFPGLVFLLEGDLGTGK
                     TTLVRGFCRADGYIGVRSPSFTLVNRYFCGEKTVVHSDLYRLSSVDPTELDVESYLDD
                     RSVLFVEWADRGVPGDFSEIWRIRFRYEDEEEDEGRRVLSFSAQGARAEKALESFLGK
                     LKERKKK"
     gene            complement(2420..3283)
                     /locus_tag="PK849_04160"
     CDS             complement(2420..3283)
                     /locus_tag="PK849_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPE65351.1"
                     /translation="MSSFSEFLPQRPASIHKGDRGGVLIVGGSSLYRGAPVLAARGAL
                     RSGCGLAVIFSEEVVCAAAASSLPECIVQAGLFTSSRKEIINVLESWESRIECLVLGP
                     GLGRSPEAGIIVRLVLEKWEKSMILDGDGIFWLSQYASVFKRRASLLLTPHEGEAACL
                     LGASIESVRCARGESARRIAVRDGIVLLKGPQTLVDDGVRCEMIDEGNQALAIPGSGD
                     VLSGSIAAFSAAGLPLFESACLGAVVHGRAGRLVSRKLGIDGVLASEVADALPLILQK
                     MRMRERETKTN"
     gene            complement(3280..3657)
                     /gene="acpS"
                     /locus_tag="PK849_04165"
     CDS             complement(3280..3657)
                     /gene="acpS"
                     /locus_tag="PK849_04165"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPE65352.1"
                     /translation="MIQGIGVDLCSISRMEKAVESAHFIHRVFSPEEIQYACGKARPA
                     RHFAGCFAAREAFSKASGISMYSLAFGGGMWIERTGSAPVVRCSHTVLDLLPHKGTER
                     IHLSLSHDGDYAVAMVVLEIGFQ"
     gene            complement(3715..5877)
                     /locus_tag="PK849_04170"
     CDS             complement(3715..5877)
                     /locus_tag="PK849_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPE65353.1"
                     /translation="MITPNGTQELQEISTPSPGQPLCPACFSPSAGSDVPCPVCGYSA
                     SQPEEGGWNRLKPGTIIHARYIVGQPLGQGGFGITYLGMDLLLNTKLAIKEYFPAGAS
                     LRNPESRCVLPASEEMSEDFRKGMEKFIEEARILARFEEHPSIVSVKDFFEENGTAYM
                     VMNHLEGRTLMAHIKERGGRLPFGEAVSILSPLMDALDEVHASGLIHRDISPDNIFLT
                     RFGQVKLLDFGASKSALALMQQRHHSVVMKKGYSPPEQYQGSRHLGPWSDVYGMAATL
                     YRCIAGMIPPDALDRMEEDPLEPPSRLGIPLPLYAERALLRALSLSSQNRPQSMREFK
                     EGLLGEMLFSSGDEKAVYGTGRKAGVDSKKESPQRAEKVKVHSAWRVRRFFTWFFFFL
                     SVLMLGAGAFFFFAASDLMERAKAGDASAQRSIGRLYEIGGILGSDQKAAEWYLEAAK
                     QGDASAQFALGRLYSAGALGAPDFTAAAEWYEKAAELGHVNAQFALALSFENGRGRAR
                     DDALAVRWYRRAAEQGDVRAQFNLGNMAEQGRGMNQSDTQAADWYHRAGSQGHTEAQL
                     RLARMYEQGRGVSQSEDSAARWYRSAAESGEVSAQFIVGTMYEEGRGVTPDPAQAAKW
                     YRKAAEQGNAAAQYSLGRLYTNGLGGKQDDVEAASLYMKAAEQGYAPAQFSLGWSYEF
                     GRGVFRNEARAAEWYRRAAAQGHTLAHIALDRLLSGDE"
     gene            complement(5936..6775)
                     /locus_tag="PK849_04175"
     CDS             complement(5936..6775)
                     /locus_tag="PK849_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPE65354.1"
                     /translation="MMIRPGNYQHIGARKEQQDAFGFSDLSDVAFVRHGGVLAIVADG
                     MGGLQEGAEASRIAVRAFLESYGGKNTDERIEDALDRSLREANNSVYAFSVERNLEGN
                     CGTTIVAVVVHESGVYWTHAGDSRLYMTDGQSLTPLTEDHIYANNLKKAVEKGLLRQE
                     DALVHPERDALTSYIGDRTIKIVGKGFLKSSPDGLPPNGFMLLLCSDGLYKTLSEKEI
                     LEVYSPDPEKWARALVKSAIAQKYRFQDNVTVLCLAAGALVPRRSEDTLNNSHAIESD
                     DVE"
     gene            complement(6788..>7178)
                     /locus_tag="PK849_04180"
     CDS             complement(6788..>7178)
                     /locus_tag="PK849_04180"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPE65355.1"
                     /translation="AAGRVASAPGGGADGGLPPFAAGGQRAAILNGIAGPYAGLQIPV
                     PLEGIIIGRDPAYSNLVIQSPAVSRRHARIMQGPSPDSCTLEDLNSTNGTFVMERNAW
                     VRLTAPAVLTIFKRFRLGDEHTEFEIR"
BASE COUNT         1587 a         2094 c         1857 g         1640 t
ORIGIN      
        1 acagaagccg ctcgatggca ccagccttct gtacacattc aagggcgcga acgccccggc
       61 gcgtcatacg acacagtact tcgagctggt cggcaatcgc ggcatctaca aggacggctg
      121 gatggcgaat acgaccccgc ttcggctgcc gtgggtgacg gtatctctcc gcggcgttga
      181 aggccttgat cgcgatctcg tagcactcct gcacggatga gggggcataa cagattgtgc
      241 catgatcgcc gtgtgttccc cagcgagcct gcatgacatc ctgctgggct gttcttgttg
      301 gcaaaccggt tgaaggaccg cctctcataa cgtcaacgac aacgatagga atctccgcga
      361 tgtaggcaaa accaagattt tcctgcttta gggagaaccc ggggccggaa gtggctgtca
      421 aagctttcct accggcaatg gagcctccaa tggttgccgc gataccggca atttcatcct
      481 ccatctggat aaaccgtccg ttaagcttag gaagttcctc cgccatgatc tctgcgatct
      541 cggtggaagg ggttatgggg tatcctgcaa aaaaacggca tccagccgca agtgctccgt
      601 gtgcaagcgc ttcgttacct tgccagaaag cgacatcagg catccttcgt atcctccttc
      661 actgtgatcg caagatcggg acagatgttt tcgcactgtc tgcatccgat gcaatcttca
      721 ggtcttgccg ctaccgattt caaacggact tcgtcaagct cgagaacttt ttttggacag
      781 atattaatac ataaggaaca tcccttacac caagaattat tcaccaaaac ttcgaatttc
      841 ttcggcaaaa aagtcacctc ctacgaattt ctcctaaaca gaaaggagcg tgcgcatctt
      901 ctagtgctct tctgatgagt aggatagctt gaacctcaac acagttcaac atcaaatata
      961 ataatttctt ttttatctct tcgattttct aaagaaaaaa atgataaaat ctatccgagg
     1021 tctggatccc taaaataatc ggcgtgtatt tcgaaagcag aacgtgcacg gcctttgtgg
     1081 cgccgcgtca attcgactgt cgcggaaccg gttggaatga ttttttcgaa ggacccgccg
     1141 aaagaaggga aaagaggggc tactttctca ggagcgtctg tgagccaaaa gacatcttga
     1201 tgacgaagct tcgtcccgga aaaagcttcg aggaataact ccggagtaaa ggatccgctg
     1261 cggaaaagac tgagcggagg aagcgtgtct ccttctcctt tgaaaagacc gccatacact
     1321 ttcccacctc ctccccaaag taccgaagca gccagaccgc cgcttcttct gatcttttcg
     1381 aaggccatgc attcaagcga agacaacgga acaatacttt ttcccgcagc ccacgcaaga
     1441 aaggtcacga aagaaattcc aaccttgatt ccggtgaagg aaccggggcc ggtgacaacg
     1501 ctgaaaagag agatgtcctc cggtgtgagg gagaatgctt tcagaaagtg ttgaaggagc
     1561 cccggaagga gagccgactg attttttccg gcatcgatgt tgatctctcc gcgtaccttc
     1621 tcgtcttccg ccagcccgag tgtggtccat gccgagcagc agtcaactga aagagtgtat
     1681 tctttcatta ttttttcttc ctctccttga gttttcccag aaaagattcc agggctttct
     1741 ctgcgcgggc gccttgggcc gaaaaagaga gcactcttcg tccctcgtcc tcttcttcgt
     1801 cttcatatcg aaaacgaatt ctccaaattt cgctgaagtc tccgggtacc cctcggtctg
     1861 cccattccac gaagagaaca ctccggtcat ccaaataact ctctacatcg agttcggtag
     1921 gatcgaccga agacaggcga taaaggtcgg agtgcactac ggttttctct ccgcaaaaat
     1981 aacgattaac cagtgtaaat gaagggctgc gcacgccgat gtatccgtct gcccggcaaa
     2041 accctcttac aagcgtcgtt tttcctgttc ccaaatcgcc ctccaggaga aatacaagtc
     2101 ccggaaagga gtgttccgcc ataagctctc caatttgcat ggtgctttcc tcggaaaaag
     2161 agatgtattc acagcatgaa atcgaatcca tcggttaagc gtcccctatc atttctgttt
     2221 tctccgcatc aacatgatga aaagaagagt cccggcggcc acaaagagaa gcaccaaagt
     2281 tagaggcccc atggaaagac tctttcttcc cattcgtcct acgccgaaaa tccagaacgc
     2341 ggcaagcgca aaactcgcga tgctgaagcg aagacctttc cagcgaactt gttcggtttt
     2401 tctaatttct tcatcccagt caattcgttt tcgtctcacg ttctctcatc ctcattttct
     2461 gaaggatcag cggaagagcg tcggcgactt ccgaagctaa cactccgtcg atccctagtt
     2521 ttcgtgatac gagccttccg gcgcgtccat ggactacagc gccgaggcat gcgctctcga
     2581 agagcggcaa ccctgcggcc gaaaaggctg caatggagcc ggaaagaaca tctcctgaac
     2641 caggaatcgc aagcgcctgg tttccctcgt caatcatttc gcagcggact ccatcatcta
     2701 caagcgtttg cggtcctttc aaaagaacaa taccgtcccg gacggcgatc cttcgggcgg
     2761 attcaccacg agcgcaacgg accgattcga tcgaagcgcc aagaaggcac gctgcttctc
     2821 cttcgtgcgg agtcaggaga agagaagcgc gacgtttaaa aacacttgca tactgcgaaa
     2881 gccaaaagat gccgtctcca tcaagaatca tggatttttc ccacttttcc agtacaaggc
     2941 ggacaattat cccggcttcc ggtgatcttc caagacccgg gccaagcacc aggcattcta
     3001 tacgggattc ccaggattcc aagacattga ttatctcttt tcgtgaggaa gtgaaaagac
     3061 cggcctgaac tatgcattca ggaagagaag acgcagcggc ggcgcaaacg acttcctcag
     3121 aaaaaataac cgctagaccg catccggagc gaagcgctcc ccgcgccgcg aggactggcg
     3181 ctcctctgta cagtgaactg cccccgacga taagcacccc gcctctgtcg cctttatgaa
     3241 tactcgccgg tctttgggga aggaattcgg aaaagctgct cattggaagc ctatctccag
     3301 gacgaccatc gcgaccgcat agtctccgtc atgagagagc gagaggtgga tccgttccgt
     3361 tcctttgtgc ggcaacagat ccaggacggt atgagaacat ctgacgaccg gagcggatcc
     3421 agtacgctca atccacatgc cgccaccgaa agccagggaa tacatggaaa tgccagaggc
     3481 cttcgaaaag gcttcgcgcg cggcaaaaca gcctgcaaaa tggcgtgctg gacgagcctt
     3541 cccgcaagcg tactgaatct cctcagggga gaaaaccctg tgaatgaagt gtgcggattc
     3601 tacggccttt tccatccgtg aaatgctgca taaatcaaca cctattcctt gaatcatatt
     3661 ctacctccaa aaacgcctta gaaaattgag ttcgacgacc tcataaagtg aagattattc
     3721 atctcccgaa agaagccggt caagagcgat gtgcgccagc gtgtgaccct gtgcggcggc
     3781 tctcctgtac cattcggccg ccctggcctc gtttcggaac actcccctcc cgaactcata
     3841 gctccacccc aaactgaact gcgcgggagc gtacccttgt tccgcagcct tcatgtacaa
     3901 agaagcggcc tcgacatcgt cctgcttccc gcctagtccg ttcgtgtaca atcgccccag
     3961 gctgtactga gccgccgcgt tcccctgctc agcagccttt ctataccact ttgcggcttg
     4021 agcaggatca ggtgtgactc cccgcccctc ttcatacata gtaccgacga taaactgggc
     4081 ggagacctct cccgattctg cagcgctccg gtaccagcgt gccgctgaat cttcgctctg
     4141 agaaacgccg cgtccctgct catacatcct tgctagtctg agctgcgctt cagtgtgccc
     4201 ctgcgaccct gccctgtgat accagtcggc ggcctgtgta tcgctttgat tcatgccgcg
     4261 tccctgttcc gccatgtttc caaggttgaa ctgcgcgcgt acatctcctt gttctgctgc
     4321 tctacggtac catcgaacag ccagagcatc atcccgcgct cttccccgtc cgttttcgaa
     4381 agagagcgcg agagcgaact gggcgttgac atgaccgagt tccgccgctt tttcgtacca
     4441 ctccgccgct gccgtaaaat caggagctcc cagcgctcct gcggaataca gtcttccaag
     4501 ggcgaactgg gcagaggcat ctccctgttt cgcggcttcc aaataccatt ccgcagcctt
     4561 ctggtcgctc ccgaggattc ccccgatctc gtacagcctt ccgatgctcc tttgtgcgga
     4621 agcgtccccc gcttttgccc gttccatcag gtcgcttgcc gcgaaaaaga aaaaggcccc
     4681 cgctccgagc atgagcaccg aaaggaagaa aaagaaccat gtgaaaaacc ttcgtactct
     4741 ccacgccgaa tgcaccttca ccttctctgc gcgctgcgga ctttctttct tcgagtcaac
     4801 gccggctttc ctcccagtac cgtaaaccgc tttctcatcg ccgctggaga aaagcatctc
     4861 tccgagaagg ccttccttga actccctcat actctgcggg cgattctgcg aggagagaga
     4921 aagagcgcgc agcagcgccc tctccgcgta cagaggaagc ggaatgccca agcgactcgg
     4981 cggttcgagg gggtcctcct ccattctgtc gagcgcatcc ggggggatca tccccgcgat
     5041 gcagcggtag agcgtcgccg ccatcccgta aacgtcgctc cacgggccca gatgcctgct
     5101 cccctggtac tgctcgggag ggctgtaccc ctttttcatg acgaccgagt gatgccgctg
     5161 ctgcatcagc gccaacgcag acttcgaggc cccgaagtcg agcagcttca cctgcccgaa
     5221 gcgcgtcaga aagatgttgt cggggctgat gtctctgtgg atcaggccgg aggcgtgcac
     5281 ctcgtcgagc gcgtccatca aaggggaaag aatgctgacc gcttccccga acggcagacg
     5341 ccctccacgc tccttgatat gcgccatcaa cgtgcgcccc tccaggtgat tcatcaccat
     5401 gtaggcggtg ccgttctcct cgaagaagtc cttcaccgag acgatggagg ggtgttcctc
     5461 gaagcgagcc agtatgcgcg cctcctcgat gaacttctcc atccccttgc ggaagtcttc
     5521 gctcatctcc tcggaagcgg gcagaacgca acggctctcc gggttgcgta gagacgctcc
     5581 ggcgggaaag tactctttta ttgcgagttt ggtgttcagc aacagatcca tgcccaggta
     5641 ggtaatgccg aagccccctt gccccagcgg ttgtccgacg atatagcggg cgtggatgat
     5701 cgttcccggt ttcagacggt tccatccgcc ctcttcgggt tgagaagcgg agtacccgca
     5761 gacagggcac ggaacatcag atcccgcaga tggggagaaa cacgccgggc aaagaggctg
     5821 tccgggactc ggtgtggaaa tctcctgcag ctcttgtgtt ccgttgggtg tgatcatgct
     5881 ctccactcct ggtgtgcggc tttttatcac ggcgtcgtca tggcacatac tgctcttatt
     5941 ctacatcatc ggattcaatg gcatgagagt tattcagggt gtcttcgctc cggcggggta
     6001 caagtgcgcc tgcggcaagg cagagtacgg tcacattgtc ctggaatcga tatttttgtg
     6061 ctatggcgct tttgacgagc gcccgggccc atttctccgg gtctggcgag tagacttcga
     6121 ggatttcttt ctcggaaagg gttttgtaaa ggccgtctga gcagaggaga agcataaagc
     6181 cgttcggcgg tagtccgtcg ggagagcttt tgaggaaacc cttcccgacg atcttgatcg
     6241 tcctgtcgcc gatgtagctt gtgagggcgt ctctctccgg gtgcacgagc gcgtcttcct
     6301 gtctcagcaa tcctttttca acagcctttt ttagattgtt cgcatagata tgatcttcgg
     6361 tgagaggcgt caggctctgc ccgtctgtca tgtacagtct gctgtctcca gcgtgcgtcc
     6421 agtaaacgcc gctttcgtgg acgacgacgg cgacgatggt agtaccgcag ttcccttcga
     6481 ggtttctctc gacggagaag gcgtagacgg agttgtttgc ctcccggaga gagcggtcaa
     6541 gtgcgtcctc tattctctcg tccgtgtttt ttcccccata cgactccagg aaagcgcgta
     6601 ccgcgatacg ggaggcttcc gctccttcct ggagtccgcc cattccgtcg gcgacaattg
     6661 caagaactcc tccgtgccgt acgaaagcta catcagaaag atcgctgaat ccgaaggcgt
     6721 cctgctgttc ttttctggcg ccgatgtgct ggtagttccc agggcggatc atcatcctcg
     6781 gcggctccta gcggatttcg aattccgtgt gttcgtctcc cagtcggaag cgcttaaaga
     6841 tggtaaggac ggcgggcgcg gtcagtcgca cccaagcgtt tctttccata acaaaagtgc
     6901 cgtttgtgga gttcaaatct tccagcgtac agctgtcggg ggaggggccc tgcattatgc
     6961 gggcgtgtct tcgggaaacc gcgggcgact ggatgacgag attggagtac gccggatctc
     7021 ttccgattat aatgccttcg aggggcacgg ggatctgcag gcctgcgtac ggtccggcga
     7081 ttccgttgag tatggccgcc cgctgtcctc cagcggcgaa gggtggaagg ccgccgtccg
     7141 ctcctccgcc gggggcggat gcgactcttc cggcggcc
//
LOCUS       DAOPKU010000092         7159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11116_length_7159_cov_5.898086, whole genome shotgun sequence.
ACCESSION   DAOPKU010000092 DAOPKU010000000
VERSION     DAOPKU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11116_length_7159_cov_5.898086"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..592)
                     /locus_tag="PK849_04185"
     CDS             complement(50..592)
                     /locus_tag="PK849_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE65356.1"
                     /translation="MEKKITIAGFGGQGVMAIGQMLGYASCKAGGNALFLPKYGPEQR
                     GGTANCTVTLADVEIGAPESRYVDTLIALNQPSLERFLGAVRPGGLVLLNSSLCRWTG
                     GREDVKLAEIPADDIAREIGSPKVANIVVIGAYIALSGVLTERQMLDAIEEKLGKRPE
                     LLEMNRLALKRGMESATPRT"
     gene            complement(595..1335)
                     /locus_tag="PK849_04190"
     CDS             complement(595..1335)
                     /locus_tag="PK849_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE65357.1"
                     /translation="MDALYERPKSLTNRATAYCPGCLHGVATRLIAELIDELDARADT
                     VAVLPVGCGTLGMFHFNTDIVVSPHGRTPAVATGVKRSAPEKLVFSYQGDGDLAAIGL
                     AEIMHAANRGENFTVIFINNGTYGMTGGQMAPTTLVGQKSTTTPEGRNPAETGYPMMM
                     CEILSQLRAPRYIARFSLDCPANVIQAKKGLRKSFELQRAGKGFTFVELLSNCPTNWG
                     LSPLECLDWMRENTMKAFPLGVFRDRED"
     gene            complement(1337..2413)
                     /gene="vorB"
                     /locus_tag="PK849_04195"
     CDS             complement(1337..2413)
                     /gene="vorB"
                     /locus_tag="PK849_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPE65358.1"
                     /translation="MEKKFLKGNEAVAEAAILAGCRFFAGYPITPQNEIPEYMARRMP
                     QVGGVFLQGESETASVCMVYGAAAMGKLAMTSSSGPGISLKAEGISTLAGAQLPAVIV
                     NVMRGGPGLGTIQAAQSDYLHATKAMGHGGFRVLVLAPSTVQEAIDLTYKAFGLAVKY
                     QNPAVVLMDGWIGSMMEPVTLPEAKEPVKNAYTGWGTRYYNPSGSDVDVVTSCLLEPD
                     RQELLNIHFGEMYETWARDEVMVEEYLLGDAEYVIVAYGTAARAARTAIGSLREKGVK
                     AGMLRPISLYPFPYDALKRLDKSRVRCILDTEMTLPVQMKDDIRLGVTDAIPVHSCGR
                     SGGVVLTNEDIEDALLRIIEEEGK"
     gene            complement(2465..2665)
                     /locus_tag="PK849_04200"
     CDS             complement(2465..2665)
                     /locus_tag="PK849_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HPE65359.1"
                     /translation="MKITINAEFCKSCGLCIDVCKRDVLEIGPETNSKGYRAVVAAHP
                     EHCVGCSVCAVMCPEGAIALKK"
     gene            complement(2668..3318)
                     /locus_tag="PK849_04205"
     CDS             complement(2668..3318)
                     /locus_tag="PK849_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65360.1"
                     /translation="MRNAVFLGPSGGGKSEISINMALRAAAEDGPAVHFFDMDQTKPL
                     FRSRTCRDLLERSGVVFHSGAEFLDSPVIPDGVADFLRDPACRCILDVGGNPAGARPL
                     GRLAALREADAAAFYVINVYRPFSDEQSLREDMERIVASARIPAPCVVSNPNLGRETT
                     LDDVLEGHRRLLPLLERLDAPLDRLCVRLPLLESVRPLVDCPVAGIALHLDGREVK"
     gene            3644..4024
                     /locus_tag="PK849_04210"
     CDS             3644..4024
                     /locus_tag="PK849_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65361.1"
                     /translation="MEKKYTALLCDDSAVVRKMLRHILNESPVGTVFEAIDGNQAVEM
                     YRLHRPDIVFMDIVMPGKTGIQALSEIRAHDPSARVIMASSTGTSKNLKAAIDSGAVD
                     FIQKPFEREAVLKLIRRILEGEWE"
     gene            4024..4914
                     /locus_tag="PK849_04215"
     CDS             4024..4914
                     /locus_tag="PK849_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65362.1"
                     /translation="MFSRLFGQFLLQEGFVTPSALSSALGRLGEVRPLIGMLALAQGY
                     MSPDQVVEINEMQKRVDRRFGELAIEKGYLSVDQLESIISAQKSEHVLLGQILVQDGI
                     LSHDGFLLVLEKYRKNAGLSEEGYEAVKANDVEGVVSSILVGQPGAGKAVVRSWATVF
                     MRSVIRFIDPGAAIDPLAEEKYKNLRGFIQRMKGDLRVSVFIAAEDEVFVDLAQRFTD
                     FDIDSFDEMASAAVGEFLNLANGLFTVNCSDEGTELDMLPQEYVESIASLPIGRPDAL
                     LPLLLPKGILWAGFCPGLSR"
     gene            5062..5538
                     /gene="grdA"
                     /locus_tag="PK849_04220"
     CDS             5062..5538
                     /gene="grdA"
                     /locus_tag="PK849_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084002417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5191..5193,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HPE65363.1"
                     /translation="MGKLTGKKLLLLGERDGVPGPAMEACLKASGASIEFSVTECFVU
                     TAAGAMDLQNQQRIKDAAEKFGAENVVVILGSSDAEGAEIYAETVSNGDPTYAGPLAG
                     VSLGLPVYHIFDGDIRNECDPAAWEEQISMMEMVLDPDALAAAVKGIRDQFSTHSL"
     gene            complement(5615..6238)
                     /locus_tag="PK849_04225"
     CDS             complement(5615..6238)
                     /locus_tag="PK849_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPE65364.1"
                     /translation="MNLVKKPKTARGAETLQRICDAAEELFAESGYYNTQISDIAKKA
                     GIASGTFYIYFPDKISAFRYLLDELGRRLRREIRAAASGARTQQEAEFVGLKTFLSFA
                     SKHIGLFKIVWQAQFVDMEAFKNYYESFAARYVTRIGDAQKRGELTEMDPEYFSYILM
                     GIYNFVALKCIVFDGKEPTDELVEEVFKFIRHGAFAPSAENGAPRPQ"
     gene            complement(6464..>7159)
                     /locus_tag="PK849_04230"
     CDS             complement(6464..>7159)
                     /locus_tag="PK849_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65365.1"
                     /translation="DPTTMERDDTPADFIAFREAFVNVLCHQDYQDHTRKPEIRRFSD
                     RTIFWNPGDAFATGDLLAPGPKEVRNPTIAVAFRRIGFSENAGWGLNDVISQWTGLGY
                     VRPVIRNGKASKDFELMLIAASSPVSSGRIQPGEGVEQVTQQVTQQVTQQVTQQVEKL
                     LAACTSELSRSELMKAVRIRDRVSFSRNYLEPALAESLIEMTQPDSPKSPTQKYRLTA
                     KGRVLLESVGSSK"
BASE COUNT         1322 a         2180 c         2209 g         1448 t
ORIGIN      
        1 cccggcggcg cggcgaatcg caagcccgtc cacggggacg gacgttgtcc tacgtcctcg
       61 gcgtcgcgga ctccattccg cgcttcagcg cgagtctgtt catttcgagc agttcggggc
      121 gcttccccag cttctcctcg atcgcgtcga gcatctgccg ctcggtgagg acgccgctca
      181 gcgcgatgta cgcgccgatg acgacgatgt tcgccacctt cggcgagccg atctcgcgcg
      241 cgatgtcgtc ggccggaatc tcggcgagct tcacgtcctc ccgccctccg gtccagcggc
      301 agagcgagct gttgagcagg acgagccccc ccggacggac tgcgccgagg aaacgctcca
      361 gcgacggctg gttcagcgcg atcagcgtgt cgacgtagcg cgactccggc gcgccgatct
      421 caacgtcggc gagagtcact gtgcagttgg ccgttccgcc gcgctgctcg gggccgtatt
      481 tcggcaggaa gagcgcgttg ccgcccgctt tgcaggaggc gtaccccagc atctggccga
      541 tggccatcac tccctgaccg ccgaagcccg cgatggtgat cttcttctcc atgatcagtc
      601 ctccctgtcc ctgaagacgc caagcgggaa ggccttcatg gtgttctccc gcatccagtc
      661 caggcactcc agcggcgaaa gcccccagtt cgtcggacag ttcgaaagca gctcgacgaa
      721 ggtgaagccc ttccccgcgc gctgcagctc gaaggacttc ctgagcccct tcttcgcctg
      781 aatgacgttc gccgggcagt cgagcgaaaa acgcgcgatg tagcgcggcg cgcggagctg
      841 cgacaggatt tcgcacatca tcatcgggta gccagtctcg gcggggttgc gcccctccgg
      901 cgtcgtggtc gacttctggc cgacgagcgt cgtcggggcc atctgaccgc ccgtcatgcc
      961 gtaggtgccg ttgttgatga agatcacggt gaagttctcc ccccggttcg cggcgtgcat
     1021 gatctccgcg aggccgatgg cggcgaggtc gccgtccccc tggtaggaga agacgagctt
     1081 ttccggcgcg gagcgcttca cgcccgtggc aacggcggga gtccgtccgt gcggcgagac
     1141 gacgatatcc gtgttgaagt ggaacatccc cagcgtgccg cagccgacgg gaagcaccgc
     1201 gacggtgtcg gcgcgcgcgt ccagctcgtc gataagttcg gcgatcaggc gggtggccac
     1261 gccgtgcaga cagccggggc agtaggcggt cgctctgttg gtcagcgact tcgggcgttc
     1321 atagagcgcg tccattttac ttcccctcct cttcgatgat ccgcagcagc gcgtcctcga
     1381 tgtcctcgtt cgtcagcacc acgcctccgg agcgcccgca ggagtgcacc ggaatggcgt
     1441 cggtcacgcc gaggcggatg tcgtccttca tctggaccgg cagggtcatc tccgtatcca
     1501 gaatgcagcg gacgcggctc ttgtccaggc gcttcagggc gtcgtacgga aaggggtaga
     1561 gggagatcgg gcgaagcatc cccgccttga cgcccttttc gcgcagagag ccgatcgcgg
     1621 tgcgggccgc gcgggccgca gtcccgtagg cgacgatgac gtactccgcg tcgccgagca
     1681 ggtactcctc gaccatgact tcgtcgcgcg cccaggtttc gtacatctcg ccgaagtgga
     1741 tattcagcag ctcctggcgg tcgggctcca gcaggcagga ggtgaccacg tccacgtcgc
     1801 ttcccgaggg gttgtagtag cgcgtccccc accccgtgta cgcgttcttc acgggctcct
     1861 tcgcctcggg gagcgtcacg ggctccatca tcgagccgat ccagccgtcc atcaggacga
     1921 ctgcggggtt ctggtacttc acagcgaggc cgaaggcttt ataggtaagg tctatggcct
     1981 cctgcaccgt actcggcgcg aggacgagca cgcggaatcc gccgtgcccc atcgccttcg
     2041 tggcgtggag gtagtcggac tgcgccgcct ggatggtgcc cagtccgggc cctccgcgca
     2101 tcacgttgac gatgacggcg ggcagctgcg cccctgcgag cgtggagatc ccctcggcct
     2161 tcaggctgat ccccggtccc gaggaggagg tcatggcgag cttccccatg gccgccgcgc
     2221 cgtagaccat gcagacggag gctgtttcgc tctctccctg caggaagacc ccgccgacct
     2281 gcggcatccg ccgcgccatg tactccggga tctcgttctg gggcgtgatc gggtagcccg
     2341 cgaagaagcg gcatccggcg aggattgcag cctcggcgac ggcttcgttt cccttcaaaa
     2401 atttcttttc cataatctca atcctttcgt gtggcggagt gcggggagcg tgcttccgtt
     2461 cccgtcattt cttgagcgca atagcccctt cgggacacat gaccgcgcat acggagcagc
     2521 cgacgcagtg ttcggggtgc gccgcgacga ccgcccggta tcctttcgaa ttggtctccg
     2581 gcccgatttc cagtacgtcc cgtttgcaga cgtcgataca gaggccgcag gacttgcaga
     2641 actcggcgtt gatggtgatt ttcaccgtta tttcacctcc cttccgtcaa ggtggagggc
     2701 gatccccgcg actgggcagt cgacgagagg acgcaccgat tccagaagag ggagccggac
     2761 gcagagacgg tcgagcggcg catcgagccg ctccagcaga gggagcagcc tgcggtgtcc
     2821 ttcaagaacg tcgtcgagcg tcgtctcccg tcccaggttc ggattgctca ccacgcacgg
     2881 agcggggatg cgggccgatg cgacgatgcg ttccatgtcc tcccggagac tctgttcgtc
     2941 ggagaacggg cggtagacgt tgatcacgta gaaagcggcc gcgtcggctt cacgcagggc
     3001 ggcgagccgg ccgagcggac gagctcccgc agggttgccc cccacgtcga ggatgcagcg
     3061 gcaggcgggg tcgcgcagaa aatccgcgac gccgtccgga atgacgggag agtcgagaaa
     3121 ttcggccccc gaatggaaga cgacgccgga gcgctccaga aggtcgcggc aggtccggga
     3181 gcggaagagc ggcttcgtct ggtccatgtc gaagaagtga acggcaggcc cgtcctctgc
     3241 ggctgcgcgc agcgccatgt tgatggagat ctccgacttg cctccgccgg aagggccaag
     3301 aaagaccgcg ttgcgcacgc catcgcctcc tagataaaaa tcaaaatatt ttatatttag
     3361 gcataaactg tgaaatcaag acggttcaaa aacgatgcta atacccccgc ctgcatttgt
     3421 caatgctcac gtttacatgc gtattcactg tgttcgagga gtcttaaggc gtgccttgcg
     3481 aaagcggagc gcgctgaaaa aacttgcgaa ctttttttaa atatccgata tttctttagt
     3541 gaataaattt catcatccat ctgcttgcac attgcgcttc atgctataat tctcaaaaag
     3601 agttcttgga tgagagaact ccatattagg gagggcttct tccgtggaaa aaaaatacac
     3661 agcgcttctc tgcgacgact ccgcagtggt gcgcaaaatg ttgcgtcata ttttaaacga
     3721 aagccctgtg ggaacggtct ttgaagccat cgacgggaat caggcggtgg agatgtaccg
     3781 cttgcaccgg ccggatattg ttttcatgga catcgtcatg ccagggaaga caggtatcca
     3841 ggcgctttcc gaaatacgtg cgcacgaccc gtcggcgcga gtgatcatgg cctcgtccac
     3901 gggtaccagc aagaacttga aagcggccat cgactccggc gcggtggatt ttattcaaaa
     3961 accgttcgag cgggaggccg tgctcaaact gatacgccgt attctggaag gggagtggga
     4021 gtagtgttct caagactttt cgggcagttt cttctccagg aggggttcgt cacgcccagc
     4081 gctctttcga gcgccttggg gcgtctcggc gaggtgcgcc cccttatagg gatgctcgcg
     4141 cttgcgcagg gctacatgag ccctgaccag gtcgtcgaga tcaacgagat gcagaagagg
     4201 gtcgaccgcc gtttcggcga actcgccatc gagaaggggt acctctccgt cgatcagctt
     4261 gaatccatca tctccgccca gaagagcgaa cacgtgctcc tcgggcagat tcttgtccag
     4321 gacggcatcc tgtcgcacga cggcttcctc ctggtgcttg aaaagtaccg caagaacgcc
     4381 ggtctctccg aagaggggta cgaggcggtc aaggcgaacg acgtcgaggg cgtggtctcc
     4441 tccattctcg tagggcagcc tggagcggga aaagctgtgg tcaggagctg ggccaccgtc
     4501 tttatgagga gcgtcatccg tttcatcgac cccggcgccg ccatcgaccc gctggcggag
     4561 gagaagtaca aaaatctgag ggggttcata caacgcatga agggcgacct tcgcgtgtcg
     4621 gtcttcatcg cagccgaaga cgaagtcttc gtcgatctcg cacagcgttt taccgatttc
     4681 gacatcgact cgttcgacga gatggcgtcg gcggcggtcg gcgaattttt gaacctcgcc
     4741 aacggtctgt tcacggtgaa ctgttccgac gaagggacgg agctggatat gctcccccag
     4801 gaatacgtcg aaagcatcgc gtcgcttccg ataggacgcc ccgacgcgct ccttccgctc
     4861 ctgcttccca agggaattct ctgggcgggg ttctgccccg gtctctcccg gtagcgcgct
     4921 ctccgtgcgc tgaaatcgca gacggcgtct cctcccttcg cggcggggcg ccgttttttt
     4981 ctttcccgcc gattttttcc gccggctctg gacgaaatcc tcatttgacg ctaccctgta
     5041 aagcagaaag gaggtggaaa aatgggcaaa ctgacaggca agaaactgct tctcctcggt
     5101 gagcgggacg gcgtccccgg acccgccatg gaggcatgtc tgaaggcaag cggcgcttcc
     5161 atcgaattct ccgtgacgga gtgcttcgtc tgaaccgccg cgggagcgat ggacctgcag
     5221 aatcagcagc gtatcaagga cgctgccgag aagttcggag ctgaaaacgt tgtcgtcatc
     5281 ctcggttcct ccgacgctga aggcgcggag atctacgccg agaccgtgag caacggcgat
     5341 ccgacctatg caggccctct cgcgggagtc tcgttgggac tccccgtgta ccatatcttc
     5401 gacggcgaca ttcgcaatga atgcgatcct gccgcctggg aagagcagat cagcatgatg
     5461 gagatggttc tcgaccccga cgcccttgcg gcggccgtaa aaggcattcg ggaccagttc
     5521 agcacacact cgttgtaaaa gtatgaagcg gcccccggcg agaaatcgcg ggggccgtgt
     5581 ttagttcgtt gaaatatgcg cgagcatgtc cggctcactg cggccgggga gctccgttct
     5641 ccgcggacgg cgcgaacgca ccgtgcctga tgaacttgaa gacctcctcc acaagctcgt
     5701 ccgtcggctc ctttccgtcg aagacgatgc acttcagggc cacgaagttg tagattccca
     5761 tcaggatgta ggagaagtac tccgggtcca tctccgtcaa ttcgccgcgc ttctgcgcgt
     5821 cgccgatgcg cgtgacgtaa cgggcggcga agctctcgta gtagttcttg aacgcttcca
     5881 tatcgacgaa ctgcgcctgc cagacgatct tgaagagccc gatgtgcttc gaagcgaacg
     5941 agaggaacgt cttgagcccg acgaactcgg cctcctgctg cgtccgcgcc ccggaagcgg
     6001 cggcgcgtat ctcccgcctg aggcggcggc ccagttcgtc cagcaggtag cggaacgcgc
     6061 tgattttgtc gggaaagtag atatagaacg tcccggaggc gatccccgct tttttcgcga
     6121 tgtcgcttat ctgcgtgttg tagtacccgc tctccgcgaa cagctcctcc gccgcgtcgc
     6181 agatgcgctg cagcgtctcc gccccccgtg ccgtcttcgg ttttttgacc agattcacgc
     6241 taccaccccg ttcgacgaat tactccgcct cacatgatag cacagaaggg gcgggagggc
     6301 gggtcccggg gaagcgagta gagagagtag tgagctctgt cctccggaga ggaggagacg
     6361 cacgcctgcg aacgttctcg tcgggcgggg cctccgtttc cttccgtcag ttgcgttgct
     6421 cttgttcaca atcggtctcc ttttccacaa cttctcgcgc acttcatttc gaactgccga
     6481 cggactccag gagcactctg cctttggctg tgagccggta cttctgggta gggcttttcg
     6541 gcgagtcggg ctgcgtcatt tcgatcaaac tttctgcaag agcgggctcc agataattac
     6601 gggagaagct gactctatct ctgatacgga cagctttcat cagttccgat ctactcaact
     6661 cggaagtgca ggctgccagc aatttttcta cttgctgggt tacttgctgg gttacttgct
     6721 gggttacttg ctgggttact tgctcgacgc cctctcccgg ctggattcgc cctgaagaaa
     6781 caggagagct cgccgcgatc aacatcaact cgaaatcctt gcttgccttg ccgttgcgga
     6841 taacagggcg gacatagcca agtccggtcc attgggaaat tacgtcgttc aagccccaac
     6901 cggcattttc actgaaaccg atgcggcgaa acgctacggc gatagtcgga ttgcgaacct
     6961 ccttcggacc gggggcgagc agatcgccgg tggcgaaggc gtcgccggga ttccagaaga
     7021 tggtgcgatc cgaaaagcgc cggatctcgg gcttacgggt gtggtcctgg tagtcctgat
     7081 ggcacagcac attgacgaag gcctctcgga aggcgataaa atcggcggga gtgtcgtccc
     7141 tctccatggt cgtcgggtc
//
LOCUS       DAOPKU010000093         7159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11119_length_7159_cov_3.706503, whole genome shotgun sequence.
ACCESSION   DAOPKU010000093 DAOPKU010000000
VERSION     DAOPKU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11119_length_7159_cov_3.706503"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..1928
                     /locus_tag="PK849_04235"
     CDS             258..1928
                     /locus_tag="PK849_04235"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPE65366.1"
                     /translation="MKALRFSRVFFACVLAVFVSTSAVACTPMGVGPKASVDGSVMVS
                     HTCDGWYDHRIQIVPGGSHQPGEMVDIFNIMCYDTRPDRPVKKVGEIPQAEKTYTYFH
                     IGYPFMNDQQVMMGEFTWSGRDELGTPEGLFYIENLEALGLARAATAREAIKVMGSLA
                     EKYGYADGGETLVIADTKELWVFEICGPGLWNKESGRPGAHWVAQRVPDDEVFVGANR
                     ARIGVIDFADTENYIFSTDITKLPEQMGWWKQGEPFHYANIFNPEPYGYPYYQSRREW
                     RALSLLAPSQKFDVLDEYEHYPFSIKPDRKLSVKDLMDIYSDHLEGTDYDMTTDAASG
                     PFGNPNRWPTPASVRPENRKGMDWERPIAMFRCSYSFVSQSRDWLPAPIGGVLWFGQD
                     APDTTVYVPIYCGVTELPKPWTTGKRAEFDRESAWWAFNLVNNWANLRWDAMYKEIRA
                     KKAEFEDVFFSLQTEVEEKALALYKKDPQEAVAYLTQYTNANLNKVEKGWWDFAFHLI
                     GKFYDGGMINEEGKMTSPGYPTEYLEKVGFGDLTVRDLERKKARETAK"
     gene            2042..2590
                     /locus_tag="PK849_04240"
     CDS             2042..2590
                     /locus_tag="PK849_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPE65367.1"
                     /translation="MKKKMMYLLLLLSIVSFPFSSLADTPKLEGPYLVTTCGQSPGAV
                     MVRMSALQAGVQAEHNNTLSASDLSGKDVKTLIVTTGTSMKGMGAAGTNVDKEIARCS
                     ELIAAAKSAGIVVVGAHVEGMARRTDSSDEASIEAVMKDADVLLVIDDSDSDGFFTEY
                     ARKHEKPLIKIKDALAIGPVLK"
     gene            2667..4001
                     /locus_tag="PK849_04245"
     CDS             2667..4001
                     /locus_tag="PK849_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HPE65368.1"
                     /translation="MTMFHHSLFVLIFIVAAFAAGKKLSLNTENSMLLSAVTGLVAHI
                     FLPKGVDPRSVLPFAEAIRHLVEGAFTYFDVCLIFMTATFFMALYKEAGGVAFIVRRI
                     VINFHNRRSVCLLLLVLVMMIPGAITGSGATTVLTVGSLVGSVLLAMDVPANKRVALI
                     FLLAAMSAACPPINLWAMMAAAGANMPYVGFARPLGLLSVTGALFAAFWLAGKGKPVD
                     MHKALSTLPEVPEGWNWRRAGAPFLVLAALVLAGRAFPYSFPVLGLPLIFMISALAVF
                     VISPSRLPLFRVAQHTVKILLGLVSIMVVVGVLIQVMTLSGVRGLLSLAVVTLPLSVL
                     FFTLWMILPISEGMLQYAVAPLFGVPLIMLFNMKGLDPVVSLAAWSVMWPLGDCLPPT
                     AVVGRAAVMELDFEGDYYRGFVKTALPVMAFILLLSTLFMVYSKEISQVIGG"
     gene            4007..4177
                     /locus_tag="PK849_04250"
     CDS             4007..4177
                     /locus_tag="PK849_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65369.1"
                     /translation="MDMLTINSVVMILYYFITTFFLLAMVRNFIVTRDVQEALLYAVV
                     MIPFVLRLLRLK"
     gene            4194..5357
                     /locus_tag="PK849_04255"
     CDS             4194..5357
                     /locus_tag="PK849_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HPE65370.1"
                     /translation="MNIRTAKLIILLFAGSCAAIAGSLFKEHMEYKEAVVASPFLSEV
                     RKLSDYSDAVKGSVNDANVYIFNGPTPGGTVVLLGGTHPEEPVANLSAQIFTENVHPV
                     QGRVIVINRINTSASMATRMGEAYPRFYHISTPWGVKKWRYGDRLATPLHSWPDPEVY
                     VHYPSGQNLAYMDVRNANRTWPGRPDGLLCERTNYALMELIRKEKADIVMDFHEAELE
                     YAVENTIVVHEKGQSVAAMASMMLTSQTFDVPIGMEFSPKALHGLSHREVGDHSDAVS
                     YLVEVAEPMLDRIRGITDEELLMSGKDRFVMKAGEYKLLYSPIDESGWPVEKRTARHV
                     ATLMTILQVNNMLAPEKTIILEGIPDYREIMGNGLGAFFQNPDEAPQERVFYD"
     gene            complement(5455..6684)
                     /locus_tag="PK849_04260"
     CDS             complement(5455..6684)
                     /locus_tag="PK849_04260"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPE65371.1"
                     /translation="MFLQQLEQAVPYVTVEPEYLEILRHPKEVLELSLPVRLDGGGVK
                     VFKAWRSHHNNALGPYKGGVRYHAEVSRDEVVALSSWMTIKCAIAGLPYGGGKGGISV
                     DIRELSRNELETLSRAYAFGLARFTGTDYDIPAPDVYTNPQIMSWFLDTYEKVKGWSE
                     SSAYTGKPPVLGGSLGRSDATGKGGVFILREGLAKLGLLGKKDLKVGIQGYGNAGTRA
                     HRFVEDDLGLTVVAVSDSGAALYNPKGLPYREVSKHKNNTRSLKGFQGAEELFPDLFF
                     GLDMDILILAALGGAVDAQNAPDIKAHVLLEIANGPVTPDAEALLTDKGVTIIPDVLA
                     NAGGVIVSCFEWIQGRSGDVWTEEYVFQKLDERLTLSFKDVMKIAEDRKISARKAAYV
                     KAVGRIVDAMRFRGIWP"
     gene            complement(6881..>7159)
                     /locus_tag="PK849_04265"
     CDS             complement(6881..>7159)
                     /locus_tag="PK849_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HPE65372.1"
                     /translation="DAILDAKGVLVVPDFLANSGGVIGSYFEWAQDLGGFFWSEEEYN
                     ERLIRIMKDNFQRVWGYAKDKNVKMRRAAFMAAIQRVADAVRMRGVFL"
BASE COUNT         1660 a         1970 c         1785 g         1744 t
ORIGIN      
        1 cacggcttgg cacaaaccgg cgaaaagatt tcttcagttt ccatcgccag ttccgtttcc
       61 ccttccccct ttttttccag ggagttttga acaaaaagaa acaaaaatgc gcacatcatt
      121 caaaacgact ggcgcaattt actgcgagca cgtagagtaa aagagtctct acgacgctcc
      181 gtttttttct tctgcggggg ggcggatttt tctgtgcgag gcaatgccaa tcacatattt
      241 ttttaggagg taaaaggatg aaagctctgc gtttttcacg tgtctttttc gcatgcgttc
      301 ttgcggtctt cgtatcgacg tcagccgtgg cgtgtactcc catgggagtt ggtccaaagg
      361 cgagcgtcga cggatcggtc atggtaagcc acacgtgcga cggatggtat gaccaccgaa
      421 tccaaattgt cccgggcggc tctcatcagc cgggagaaat ggttgatatt tttaatatca
      481 tgtgctacga cacccgcccc gaccgccctg tgaaaaaggt cggcgaaatt ccccaagctg
      541 aaaagactta tacctatttc catatcggct accccttcat gaacgaccag caggtcatga
      601 tgggcgagtt cacctggagc ggacgggatg aacttggaac tcctgaaggg cttttctata
      661 tcgaaaatct ggaagccctc ggtctggcgc gagcagccac cgcgcgggaa gccatcaaag
      721 tcatgggttc ccttgcagaa aagtacggtt atgcggacgg cggagaaacg cttgtcatcg
      781 cggatacgaa ggagctttgg gtattcgaga tctgcggccc cggactctgg aacaaagagt
      841 ccggccggcc cggcgctcac tgggtagcgc agcgtgttcc cgacgatgaa gtattcgtcg
      901 gagccaaccg ggcgcgcatc ggcgtgatcg acttcgccga caccgaaaac tacattttct
      961 ccaccgacat caccaagctc cccgagcaga tggggtggtg gaaacaggga gagcccttcc
     1021 actatgcaaa tatcttcaat ccggaacctt acggctatcc gtactatcag tcccgccgcg
     1081 aatggagagc tttgagcctt ctcgcccctt cacagaaatt cgacgtcctc gacgagtatg
     1141 agcactaccc attctccatc aaaccggatc gtaaactgag tgtaaaagat ctcatggaca
     1201 tctacagcga ccatcttgaa ggtaccgatt acgatatgac cacggacgcg gcttccggcc
     1261 cctttggaaa tcccaaccgg tggccaactc ccgcttccgt tcgccctgag aaccgtaagg
     1321 gaatggactg ggagcgtccg atcgcaatgt tccgctgctc ttacagcttt gtttcacaga
     1381 gccgcgactg gctccccgcc ccaatcggcg gtgtgctctg gttcggacag gacgccccgg
     1441 acacaacggt atacgttccc atctactgcg gtgtaaccga gctgcccaaa ccctggacca
     1501 cggggaaacg tgcggagttc gatcgtgaaa gtgcctggtg ggccttcaac cttgtgaaca
     1561 actgggccaa tctgcgctgg gacgccatgt acaaagagat cagagcaaag aaggcggagt
     1621 ttgaagacgt cttcttctca ctgcagacgg aagttgagga aaaagcgctt gccctgtata
     1681 agaaagaccc tcaggaggca gtcgcgtatc ttacccagta cacaaatgcc aatctgaaca
     1741 aagtcgaaaa gggctggtgg gatttcgcat tccatctcat cggtaaattc tatgacggcg
     1801 ggatgatcaa tgaagaggga aagatgacct cgccgggtta ccccacagag taccttgaaa
     1861 aagtcggatt cggcgatctc acagtccgcg acctcgaaag gaaaaaagcc agagaaaccg
     1921 cgaaatagtc ggagtgcgtc aggtaaatcg gctgcccccc ccgcagccga tttactataa
     1981 aaataagccc ggatgaaaaa acacgcgccg cgagaataca cttttcagga ggtgctcata
     2041 gatgaaaaaa aagatgatgt atttgcttct cttgctttcg attgtttctt ttccgttttc
     2101 cagccttgcc gatactccga aacttgaagg tccttatttg gtcaccacct gcggacaaag
     2161 tcccggggcc gtcatggtgc gcatgtcggc gcttcaggct ggagtgcagg cggaacacaa
     2221 caacacgctt tccgcttcgg atctttccgg aaaagacgtg aaaacgctta ttgtcactac
     2281 cggtacaagc atgaagggta tgggagctgc gggaacgaac gtagacaagg aaatcgcccg
     2341 gtgcagcgag ctcatcgctg cggcaaaatc tgccggaatc gtggtcgtcg gcgcgcatgt
     2401 agaaggaatg gcccgccgga ccgacagctc cgacgaagcc tctatcgagg ccgtcatgaa
     2461 ggatgccgat gtgctccttg taatcgacga cagcgacagc gacggatttt tcactgaata
     2521 tgcgcggaaa cacgaaaaac cattaatcaa aattaaggat gctttggcaa tcggtccggt
     2581 cctcaagtag ccatctttat tgttggcatg agggcctctc tttgaggccc tttttgcttt
     2641 acattttctg aaaggattgt attccgatga caatgtttca tcattctctc tttgtattaa
     2701 tttttatagt tgcagctttc gctgcaggaa agaagctctc tctgaacacc gaaaactcga
     2761 tgcttctctc ggcggtcaca ggactcgttg cgcatatttt tctgccgaaa ggagtcgatc
     2821 ccagatccgt actcccattt gccgaagcaa tccggcatct tgttgaaggc gcctttacgt
     2881 atttcgacgt ctgccttatc ttcatgacag caactttttt catggctctg tataaggaag
     2941 caggcggcgt tgcatttata gtgcgccgca tagttattaa ttttcataac cgcagatccg
     3001 tatgcctcct tcttctcgtc cttgtgatga tgatcccggg ggccattaca ggatccggcg
     3061 ccacgaccgt gctcacagta ggctccctcg tcggttcggt gttgcttgcg atggatgtcc
     3121 ctgcaaataa aagagtcgcc ttgatctttt tgctcgcagc tatgagtgca gcctgcccgc
     3181 cgatcaacct gtgggcgatg atggccgctg caggagcaaa tatgccctat gtgggctttg
     3241 cccgtccgct cggacttctc tcagtaacgg gcgcgttgtt cgcagcgttc tggcttgcgg
     3301 ggaaaggtaa acctgtcgat atgcacaaag cgctctcaac gctcccggag gtccctgaag
     3361 gctggaattg gcgccgtgcg ggcgctcctt ttctcgtcct cgcggcactc gttctcgccg
     3421 gaagagcgtt cccctattca ttcccggttc tgggactgcc gctgattttt atgataagcg
     3481 ccctcgcagt cttcgttatc agtccctctc gccttccctt gttccgtgtg gcgcagcata
     3541 ctgtaaaaat cctgctcgga cttgtgagta tcatggtggt tgtaggcgtt cttattcagg
     3601 tgatgactct cagcggcgtt cgggggcttc tctctcttgc cgtagtaacg cttccgctca
     3661 gcgtgctttt ctttactctc tggatgatac tgcccatatc ggagggaatg cttcagtacg
     3721 cagtcgcccc gctcttcggc gttcctttga tcatgctgtt caacatgaaa gggcttgatc
     3781 ccgtagtttc ccttgcagcg tggtcggtca tgtggccgct gggcgactgt cttccgccta
     3841 ctgcggtagt cggacgtgcc gctgttatgg agcttgactt cgagggagac tattaccggg
     3901 ggtttgtcaa aacagctctt ccggtgatgg cttttattct cctcctgtcc actctcttca
     3961 tggtgtacag caaggagatt tcccaagtga tcggaggttg acgtctgtgg atatgcttac
     4021 gataaattca gtggtgatga tcctgtatta cttcatcaca actttttttc ttcttgcgat
     4081 ggttcgaaac ttcatcgtga cacgggatgt tcaagaggct ctcctgtacg ccgtcgttat
     4141 gattcccttt gttttgcgcc ttctgcggct caagtagaaa ggggccttag agaatgaata
     4201 ttcgcactgc aaaactcatc attcttcttt tcgcaggctc ttgcgctgcg atcgcaggat
     4261 ctctttttaa agagcatatg gagtataaag aagctgtcgt cgcgagcccc tttctctcag
     4321 aagtcagaaa actcagcgat tacagcgatg cagtcaaagg ctcggttaac gatgcgaatg
     4381 tatatatttt caacggtccg acccccggcg ggaccgtcgt cttgctcgga ggaactcacc
     4441 cggaagagcc ggttgcgaac ctttcagctc agatatttac cgagaacgtt catcccgttc
     4501 aaggacgggt catcgtcatc aaccgcatca acacaagcgc ctccatggcc acacgaatgg
     4561 gagaggcgta ccccaggttt tatcatattt ccaccccctg gggagtaaag aaatggagat
     4621 atggcgaccg gctcgcaacc ccgttgcact cctggccgga ccccgaggtg tacgttcact
     4681 acccgagcgg acagaatctc gcctacatgg atgtacggaa cgcgaaccgg acatggccgg
     4741 gacgccccga cggcctcctt tgcgaacgta cgaattacgc gctcatggaa ctgatccgta
     4801 aagaaaaagc ggatatcgtc atggacttcc acgaagccga gcttgaatac gccgtggaga
     4861 acaccatcgt cgttcacgaa aagggacaat cggtagcagc catggcatct atgatgctca
     4921 cctcccagac ttttgacgta cctattggta tggagttctc cccaaaagcg cttcacggtc
     4981 tttctcaccg cgaggtcgga gatcattccg acgctgtttc ttaccttgtt gaagtggcgg
     5041 aacctatgct tgacagaatt cgcgggatca ccgacgaaga gctcttgatg tcaggaaagg
     5101 accggtttgt catgaaggcg ggagaataca agctcctcta ctcccccatc gatgagagcg
     5161 gatggcctgt ggaaaaaagg acggctcgtc atgtcgctac gctgatgacc attctccagg
     5221 tgaacaacat gctcgccccg gagaaaacga tcatccttga aggaatcccg gattaccggg
     5281 aaataatggg aaacggcctg ggggccttct ttcaaaatcc agatgaggcg cctcaagaga
     5341 gggtttttta cgattgatcg tatagagagg aacatctatc ttcataaacc cctctctaca
     5401 gaagagcgga gaccgactca aagaaaagaa tccggcctcc gctcttcttc atttttaggg
     5461 ccatatcccc ctgaatcgca tcgcatcgac gattcttcca accgccttga catatgccgc
     5521 cttcctggcg cttatctttc tgtcctcagc gattttcatg acgtccttaa atgaaagagt
     5581 aaggcgttcg tcaagcttct gaaaaacgta ctcctccgtc catacgtctc ctgatctccc
     5641 ctgtatccat tcgaagcaac tgacgataac ccctcccgca tttgcaagaa catccggaat
     5701 aatcgtaacg cctttatcgg taagcagggc ctcagcatcg ggcgtaaccg gaccgtttgc
     5761 aatttcgagc agtacatgcg ctttaatgtc cggcgcattc tgagcatcca cagcgcctcc
     5821 aagtgctgcg aggatcaaaa tatccatgtc aagtccgaag aaaagatcgg ggaagagctc
     5881 ctcagctccc tgaaatcctt taagggaacg ggtattgttc ttatgtttag agacttctct
     5941 atacgggagc cctttcgggt tatagagtgc cgccccgctg tctgatactg caacaaccgt
     6001 tagtccaaga tcatcttcca caaagcggtg agcacgcgtc cccgcgttcc cgtagccctg
     6061 tattccaacc ttcaaatctt tcttccccag aaggccgagc tttgcaagcc cctcacggag
     6121 aataaagacg cctcccttcc ccgtagcatc cgatcttccc agagaacccc ccagaacagg
     6181 aggtttcccg gtatatgccg acgattcgct ccatcctttg actttttcat acgtatcaag
     6241 gaaccacgac atgatctgag ggtttgtgta cacatccggc gccgggatgt cgtaatccgt
     6301 ccccgtaaat cgggcaagtc cgaaagcata cgctctgctc agagtttcaa gttcgttgcg
     6361 ggaaagctcc cttatatcga cagagattcc tccctttcct cctccgtaag ggagccctgc
     6421 aattgcacat ttgatcgtca tccacgatga aagggcgacc acttcatccc tgctgacctc
     6481 cgcatgatag cgcacgccgc ccttgtaagg gccaagagcg ttgttgtggt ggctcctcca
     6541 cgccttgaac accttcacgc ctcctccgtc gaggcgcaca ggcaaagaga gttcaagcac
     6601 ctccttcgga tgcctgagaa tttccaggta ctccggctcc acagtcacat acggaaccgc
     6661 ctgttcaagc tgctgtaaaa acatagcctg cggattcacc tttcatccct ccaatcttct
     6721 ggaaacgaac tgctactgga cgctcacaac ttaattgaac agttggaatt cgtgtattat
     6781 tttatcacac aactcttttc ctgccccact tttcaataaa aaaaacgacc ggttgcccgg
     6841 tcgttttcag atgtatcaga aaaaaagttt gagctcgaac ttacaggaag acgcctctca
     6901 tgcgaactgc atcggcgact ctctgaatcg ccgccatgaa cgctgcgcgg cgcatcttca
     6961 cgttcttgtc ctttgcgtag ccccacacgc gctggaagtt gtccttcatg atgcggatga
     7021 ggcgctcgtt gtactcctcc tccgaccaga agaaaccgcc gagatcctgc gcccactcga
     7081 agtaagagcc gataacgccg ccggagttgg cgaggaagtc agggacaacg agaacgccct
     7141 ttgcgtcaag aatagcgtc
//
LOCUS       DAOPKU010000094         7120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11205_length_7120_cov_5.132767, whole genome shotgun sequence.
ACCESSION   DAOPKU010000094 DAOPKU010000000
VERSION     DAOPKU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11205_length_7120_cov_5.132767"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PK849_04270"
     CDS             <1..766
                     /locus_tag="PK849_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPE65373.1"
                     /translation="HEASVLGVQGVSDEEWKETVGIALVIGGDGTFLRAARYILGASI
                     LLYGINLGHLGFLASGKAEEAIQDVESILRGEYSVQTHRLLDGRISRDGHDTYSLYAL
                     NDLVLAKGALARVMHIEVHIGDKLLNTLPADGIIVSTPTGSTAYALSAGGPIIPPHVP
                     CMLVVPICAHTLYSRPVVAGEDDTVTLVPRGNHRDLLLTQDGQLGYEILPGDRIEISL
                     SRTQKIDVLLLPSRNYFDLLQEKLRWGQGIVTFEKE"
     gene            772..2502
                     /locus_tag="PK849_04275"
     CDS             772..2502
                     /locus_tag="PK849_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE65374.1"
                     /translation="MIEEISIKNVGGIASISMRLETGFTVITGESGAGKSSLVRALEL
                     IGGKRSQALFIRSGEEEASVEAVFSGSAERVSKTLQPRDEDGLLFARRILSRGGRNRC
                     FFQDKATPFSTFASLMNDRMRIQSQFAQIELLDPKRQMDILDFCCGAQAEHLRAALEN
                     TFDEALRCDRELRAVKSKEQELKLRFRDAEAIVEALRPLQISPGCDLLWERELDEKNR
                     TFQQARKVRENLLELTGGASNDGMIDRLESCALELIRRLFLEKTDVEASFNEGLSFLH
                     SFISSIEEKASALSPEKIERERDLIEKKLGILRKIKRTTGTRTAEELVSWTSDAQEAI
                     GWLREEGRLSSELLLCAKKLRREASSLAMELRALRQRAADSLEKEVNRHLQELGMAES
                     VFSVKLLPLDKIRSSGADEISFTLSSGGSEEMPVNKSASGGELSRILLALQLALPEET
                     LPPTLIFDEVEAGLGGKAALLAGYKLLALSRKTQVILVTHEATIASLADHHFLVKKTT
                     DCVEVKKLERDDRIGEVARMLSGDASLQEAREHAERLLYSAESTECSQSGNISVLAGE
                     GDLAALRGKV"
     gene            2611..2928
                     /gene="rplU"
                     /locus_tag="PK849_04280"
     CDS             2611..2928
                     /gene="rplU"
                     /locus_tag="PK849_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPE65375.1"
                     /translation="MYAIVETGGKQYRVQEGDLLKVESLGLEEGASVVFDKVLLLGRD
                     DAVVVGAPYVKGATVTASVTGNGREPKILVFKFKSKKNYRRMRGHRQNYSEIRIDTLT
                     AGV"
     gene            2931..3272
                     /locus_tag="PK849_04285"
     CDS             2931..3272
                     /locus_tag="PK849_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HPE65376.1"
                     /translation="MTEISVSFDEERPVALVAEGHSGYAERGEDIVCAGVSALLQALL
                     FGFEKVVSSPGFSFSINEDSARMVLDWRRSPPEQTAVLVETIVGSLKEIARVYPEHVR
                     ILEVPVDEMEF"
     gene            3256..3543
                     /gene="rpmA"
                     /locus_tag="PK849_04290"
     CDS             3256..3543
                     /gene="rpmA"
                     /locus_tag="PK849_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPE65377.1"
                     /translation="MKWNFDLQLFAHKKGQGSSTNGRDSEGKRLGIKIYAGQTVQAGN
                     ILVRQRGTKIHPGKNVGLGKDYTIFATSAGTVRYETKGSRKIVSVVLQAAE"
     gene            3703..5070
                     /gene="obgE"
                     /locus_tag="PK849_04295"
     CDS             3703..5070
                     /gene="obgE"
                     /locus_tag="PK849_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPE65378.1"
                     /translation="MKFVDFTRISVRGGRGGNGCLSFRREKFIPKGGPDGADGGRGGD
                     IILEAAPGALTLADFEYEKKFQAGNGEPGKGGMKSGGAGNDKIVIVPCGTIVYDDDTG
                     EILADLVEPGDRLIAAKGGRPGKGNAHFANSVRKSPRFAEKGDEGEARTLLLELKLIA
                     DVGLVGVPNAGKSSLLGAISSARPKIAGYPFTTLSPNLGVLSVDDQKIVIADVPGLIE
                     GAHENKGLGVYFLRHVERTRFLVHVLDLSAGTADEVIEQWSIIRNEFEAYSNSEESGL
                     PGYHRQDLLLRPYIVVGNKIDLENAAENDMHIRSFMEEQGIPYFSVSALSGEGIPEFI
                     QLIAEYARENQRHEGETRLAPLVVEIQEPRRWRRKLEPVTIIKLGDGAGFRVVHANLE
                     KTLLRYDFEQEDALPKFARLVKKFRIEERLEEMGAVKGDKVFIGDHEFDFEPDMAGAS
                     RSENR"
     gene            5128..5721
                     /gene="nadD"
                     /locus_tag="PK849_04300"
                     /pseudo
     CDS             5128..5721
                     /gene="nadD"
                     /locus_tag="PK849_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
     gene            5744..7027
                     /locus_tag="PK849_04305"
     CDS             5744..7027
                     /locus_tag="PK849_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPE65379.1"
                     /translation="MKILRIVAILLLAMGAAFAGAAFKVKTFVEPQPQTIRESISFTE
                     ESGSINLLLIGIDDVDGGRRADAIAYATIDIDNKIVRLMSIPRDTRVQLPKRGWEKIA
                     HAYAYGGVELLRETVVNYLGMPINYYVLVNYDTFPSIVDLIGGVEVDVPKRMVYNDYS
                     GKLFINIPKGLQVLDGKNALHYVRFRHDALGDIGRVKRQQDFIKAVLAKLQSPYMIPK
                     IPDLANKAIELVNSDMTPAQGLQLASYLVSMPSENIHFFTMPGKAAYISNISYWVGDI
                     TAASALLTGVPEGHGTLSGDVAKEETDMGTLDLSTTDVPALISSINTPVAVLNGAGVS
                     GLGKTMATALQKIGIDVTHVGNARHFDYRTTTIHLPEQTTDPVAETGKTLAELLSVNP
                     RLISRTANVSHATIIIGKDHALVLKRLEELAEKKQ"
BASE COUNT         1790 a         1649 c         2032 g         1649 t
ORIGIN      
        1 gcatgaggcg tcggtgctgg gagtccaggg ggtgagcgac gaagagtgga aagagactgt
       61 cggaatcgcc ttggttatcg ggggcgacgg gacgtttttg cgagctgccc ggtatatttt
      121 gggcgcctcg attctcctct acggaatcaa cttggggcat ctcggttttc tggcttccgg
      181 aaaggccgag gaggcgatac aggatgtgga aagcatcctc cgcggggagt actccgtgca
      241 gacgcacaga ttacttgacg gcagaatctc acgagatgga cacgatacct attccttgta
      301 cgctctcaat gatcttgtcc tggctaaagg agcgcttgcc agggtaatgc atattgaagt
      361 gcacattggc gacaaacttc tgaacacgct cccggccgac ggcataatcg tgtcgacgcc
      421 gactggatcg accgcgtatg ccctctccgc aggcggtccg attatccccc cgcacgttcc
      481 gtgcatgctt gtcgtgccaa tatgcgctca tacgctctat tccagaccag tagtcgcagg
      541 agaggatgac acagttaccc tggttcccag aggaaatcac agggatttgc ttcttactca
      601 ggatggtcag ctaggctatg agatccttcc gggggatcgt atagaaatct cgttgagccg
      661 cacccaaaag atcgatgttc tgctcctccc gtcacggaat tacttcgatc tgcttcagga
      721 gaaactccgc tggggacagg gcattgtcac ctttgagaag gagtgacggg gttgatcgaa
      781 gagatctcca ttaagaacgt aggaggtata gcctccatat cgatgcgcct ggagaccggc
      841 ttcacagtga tcaccggaga gagcggcgcc ggaaagagca gccttgtcag ggctttggaa
      901 ctgatcggcg ggaagaggag tcaagcgctt tttattcgct ccggcgagga agaggcgtcg
      961 gtggaggccg tcttttccgg atctgccgaa cgagtttcga aaacgctgca accgcgagac
     1021 gaggacggac tgctctttgc gcggcgtatt ctttcaagag gaggaagaaa ccgatgtttc
     1081 tttcaagaca aagcgactcc cttctctaca tttgcatctt taatgaacga caggatgagg
     1141 attcaaagcc agtttgccca aatagagctt cttgatccaa agcgtcagat ggatattctc
     1201 gacttctgct gcggcgctca agctgagcat ttgcgtgccg cgctcgaaaa tacatttgat
     1261 gaagcgctcc gttgcgaccg ggaattgaga gcggtgaaat cgaaagaaca agagttgaag
     1321 ctgcgttttc gcgatgcgga ggcaatagta gaagctcttc gcccgttgca aatctccccg
     1381 ggttgcgacc tcctttggga aagagagctt gatgaaaaaa accgcacctt ccaacaggcc
     1441 cgaaaagtca gggaaaatct ccttgaattg accggagggg cttccaacga cggaatgatc
     1501 gacagactcg aatcgtgtgc gctggaactc atacgccgtc tctttctgga gaagaccgac
     1561 gtcgaagcgt ctttcaacga agggttgtct ttcctgcact cttttataag ctccatcgaa
     1621 gagaaggcct ccgctctctc accggaaaaa attgagcgcg aaagggatct cattgagaaa
     1681 aaactaggaa tacttcggaa gattaaacga acgacaggaa cccgcaccgc cgaagagctt
     1741 gtttcctgga cttcggacgc gcaagaggct atcggctggt tgagggagga agggcgtctc
     1801 tcttcggagc tgcttctatg tgcgaaaaaa ttacgccgcg aggcaagctc attggcgatg
     1861 gagctgcgcg cactccggca acgggcagcg gattcactgg aaaaagaagt gaataggcac
     1921 cttcaggagc tcggcatggc cgagagcgtt ttttcggtga aacttttgcc gttggacaaa
     1981 atacgcagca gcggggctga cgaaatttca tttactctct cctcgggagg ctctgaagaa
     2041 atgcctgtca ataaatctgc ttccggaggc gaactgagta gaatattgct cgcgctccaa
     2101 cttgcgttgc ctgaggaaac gctccccccg actcttatct tcgatgaagt ggaggccggg
     2161 cttggcggaa aggctgcgct tctggccgga tacaagctgc tcgctctctc cagaaaaaca
     2221 caggtgatcc ttgtaacgca cgaggcaacg attgcatcgc tcgcggatca tcactttctc
     2281 gtcaagaaaa cgacggattg tgtagaagtc aaaaagctgg aaagagacga ccgcatcgga
     2341 gaagtagcac ggatgctctc aggagacgcc tctttgcagg aggctcgaga acatgccgaa
     2401 cggcttctct actccgcgga gagtacggag tgcagccaat caggcaatat aagcgtcctt
     2461 gccggagagg gtgatctcgc tgccctgcga ggaaaagttt gactttaacc ttttcgctcc
     2521 gtataatact acggttggat gtttaaatcg cacgggacag gttgaagctg ctgagcagcg
     2581 cctggtcttc ccggcgggag gaggaaaaca atgtacgcga tcgttgaaac aggaggcaaa
     2641 cagtaccgtg ttcaggaggg cgatcttttg aaagtcgaat cactcggtct tgaagaaggc
     2701 gcctcggtgg tttttgataa ggtccttctt ctaggaaggg atgacgctgt tgtagtcggc
     2761 gccccctatg tcaagggagc tacggtgacc gcttcggtga cggggaatgg cagagaaccg
     2821 aagatcctcg tttttaagtt caagagcaag aaaaactatc ggcgtatgag agggcacaga
     2881 cagaattact ctgagatacg aatcgatact ctgactgctg gagtttgatt ttgaccgaga
     2941 tctccgtttc cttcgacgag gagcgccctg tcgctcttgt cgcagaaggt cattcggggt
     3001 atgccgaacg gggcgaagat attgtatgtg cgggcgtctc cgcactttta caggcgcttc
     3061 ttttcggttt tgagaaggtt gtttcttctc ccggtttctc tttcagcatc aatgaggaca
     3121 gtgcgagaat ggtattggac tggagacgat cccctccgga acagacggcg gttctcgtgg
     3181 aaactatagt gggatccttg aaggaaatag cccgggtata cccggagcat gtccgtattc
     3241 tggaggtgcc tgttgatgaa atggaatttt gacctgcagc tctttgccca caagaaaggg
     3301 cagggaagca gtacgaatgg aagagacagc gaaggaaaga ggctcgggat aaagatatat
     3361 gccggccaga cggtacaggc gggcaacatt ttggttcgcc aaagaggaac aaaaatccat
     3421 cctggcaaga acgtcggtct tggtaaggac tatacgattt ttgcgacaag cgctggaacc
     3481 gtgcgttacg agacaaaagg gagcaggaaa atagtttcag tagttctgca agcggcagag
     3541 taattctccg gactttttga acacgacgca ttcaacaagg gccttcgcat caccttaaga
     3601 acagaccgtc tgctttttac gtcggctctc gcccttaggg gatcatcaag gcccttgatt
     3661 ttttgaaatg ataaaggaaa aggcctttcg gccggtgatc acatgaaatt tgttgatttt
     3721 acacgaatat ctgtccgcgg aggtcgcggg gggaacgggt gtcttagctt ccgtagggaa
     3781 aagtttatcc cgaaaggcgg tcctgacggc gccgacggag gaagaggggg cgacatcatt
     3841 ctggaggcgg ctccgggagc gttgacgctt gccgattttg aatatgaaaa gaaattccag
     3901 gccggaaacg gagaaccggg caagggcggt atgaaatcag gcggagcggg gaacgacaaa
     3961 attgtcatcg ttccctgcgg taccattgtc tatgacgacg ataccggcga gatacttgcc
     4021 gatcttgtcg agcctggaga tcgccttata gcggcgaagg gcgggcgccc cggaaaagga
     4081 aacgcgcact ttgcgaactc ggtgcgaaaa tcgccacgtt tcgcggaaaa gggcgatgaa
     4141 ggcgaggcgc ggacgctgct gctcgagctc aagctgattg cggacgttgg tcttgtcggc
     4201 gtacccaatg ccgggaaatc cagccttcta ggagcgattt cttctgcacg ccctaaaatt
     4261 gcgggatacc ctttcacgac cctttcaccg aatctggggg ttctctcagt cgacgatcaa
     4321 aagattgtga ttgccgatgt tccgggactc attgaggggg cccatgagaa caaggggctt
     4381 ggcgtctatt tccttcgtca cgttgagcga acgcgttttc ttgttcatgt tctcgacttg
     4441 agcgccggga ctgctgatga agtgatcgaa cagtggagca tcatccgcaa cgagtttgag
     4501 gcctacagca attctgaaga gagcggtctt cccggatacc atagacagga tcttttactg
     4561 agaccgtata ttgtagtagg gaataaaata gatcttgaaa acgctgcgga aaacgatatg
     4621 catattcgtt catttatgga ggagcagggg ataccctatt tttcagtaag cgctcttagc
     4681 ggagaaggta taccggaatt catacagctc atagcggagt acgcgagaga aaaccagcgg
     4741 catgaaggcg agaccaggct tgcgccgctt gtagttgaaa ttcaggagcc tcgtcgttgg
     4801 agacgcaagc tcgaacctgt cacaatcata aagctcggcg acggagcggg attccgtgtt
     4861 gttcatgcga atctcgagaa aacgcttctt cggtatgact ttgaacagga ggacgcgctt
     4921 cctaagtttg cccggctcgt gaaaaagttc cgcattgagg aacgtcttga ggaaatgggc
     4981 gcagtaaaag gtgataaggt atttataggc gatcacgaat tcgactttga accggatatg
     5041 gcaggggctt ctcgttcgga gaacagatga agcaggggag tcggaagcga aggagttgac
     5101 gacgtgtcgt attctgtacg caatgatgtg cgaagaatag gcattatggg cgggactttc
     5161 gatccaattc acttcggcca cctattagct gcggaagaaa gccggactgc tgtcggtctt
     5221 gatgaggtga ttttcgtacc tgctggagac ccggcgcata aacgtcagcg tcgcgttgct
     5281 ttctgtgaag accggtatat catgaccctc ttaacgacgt tggagaatcc taattttacg
     5341 ccttcgcgga tcgagatcga cagaaaggaa ccgagtcata ctgtcgatac tcttcgggag
     5401 atgcgtcact ggtatgctcc cgaaactgta gagtttttct tcattacggg gctggatgct
     5461 gtcttgagca tggcaacatg gaaagaatac gctgaactcc cgaagttatg ccgtattgtg
     5521 gcggttaatc ggcccggata tgcccagaac gcgttggata tgcttccgcc gactctccgg
     5581 gatgcaatca ttccactcga aattccgctc ctctctattt cgagtacgga aatccgccag
     5641 cggatagaga cggggaggag cataaaatac cttcttcctg aaacggtgga gcagtatata
     5701 tataagaaag gactctaccg gaaaaactgg agggcgataa acaatgaaaa tactgcgcat
     5761 agtggcaatt cttctcttgg caatgggagc ggctttcgca ggggcagcct tcaaggtgaa
     5821 gacgttcgta gagcctcaac cgcagacaat tagggagtct atctctttta cggaggagtc
     5881 gggatctata aatctcctcc tcatcggtat tgacgatgtc gacggagggc gacgcgcgga
     5941 tgcgatcgca tatgcaacca tcgatatcga taataagatt gttcgtctca tgtcaatacc
     6001 gcgcgatacg cgtgtccagc tgccgaaaag aggctgggaa aaaattgccc atgcgtatgc
     6061 atatggaggg gtcgaacttc tacgtgaaac agttgtaaac taccttggca tgccgatcaa
     6121 ctactacgtt ctagtgaatt atgatacttt cccctctata gttgatctta tcggaggcgt
     6181 cgaagtcgac gtgccaaaac gcatggtcta caatgactat tcgggcaaac tttttatcaa
     6241 tatcccaaag ggtttgcagg tgctcgacgg gaagaatgca ctccattacg ttcgattccg
     6301 tcatgacgcg ctgggggata ttggtcgtgt caagcgccag caggacttta taaaggctgt
     6361 tttggcgaaa ttgcagagcc cttacatgat accgaagata cctgacttgg cgaataaagc
     6421 gattgagttg gtgaactcgg atatgacgcc tgctcagggg ttacagcttg cgtcctacct
     6481 tgtttcgatg ccgagtgaaa acatacattt ttttaccatg cccgggaagg ccgcgtacat
     6541 ctcgaatatc agctactggg ttggcgacat cacagccgcc tccgcgctgt taacgggggt
     6601 tccggaagga cacggaacgc tatccggaga tgtggcgaaa gaagagaccg atatgggaac
     6661 gcttgatctc tctacaacgg atgttccggc attgatctct tcaataaata cgcctgtggc
     6721 tgttttaaat ggcgccggcg tctcaggtct tggaaagaca atggcgacgg cattgcaaaa
     6781 gattgggatt gatgttaccc atgttgggaa tgcgcgtcac tttgattatc gtacaacaac
     6841 gatacatctt ccggaacaaa caaccgatcc ggttgcagag actggaaaga cgctggcgga
     6901 gcttctcagc gtgaaccctc gtctaatttc acgaactgca aatgtttcgc atgctacaat
     6961 aatcatagga aaggaccacg cgcttgtgtt gaagcgattg gaagagttag ctgaaaaaaa
     7021 acagtaagtg taggggaggg tgacagcttg aagaaacagg atgaccggct caaggagtat
     7081 gaatttctgt acgaagcgct ggcggacaag agagggcttg
//
LOCUS       DAOPKU010000095         7099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11242_length_7099_cov_4.468200, whole genome shotgun sequence.
ACCESSION   DAOPKU010000095 DAOPKU010000000
VERSION     DAOPKU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11242_length_7099_cov_4.468200"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PK849_04310"
     CDS             <1..238
                     /locus_tag="PK849_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65380.1"
                     /translation="AVSNLTMGITTIDFIWDVSRDNPEALYKGRAAARLELDRLIKRI
                     EKLNAEKRELEGKLSELNAELDTITHRVEELQRQ"
     gene            450..674
                     /locus_tag="PK849_04315"
     CDS             450..674
                     /locus_tag="PK849_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65381.1"
                     /translation="MQTSLQERKQLYRRIRGLSEDKYAKVVKFVLSMENEEPPLSAEE
                     EEDLRLAYAQMKSGDYRSFDEAMKELWESE"
     gene            667..927
                     /locus_tag="PK849_04320"
     CDS             667..927
                     /locus_tag="PK849_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65382.1"
                     /translation="MNEWTLRIMAIAEKRISRIPNPEKKRILMAIDELRYDPFAHDVI
                     SLSGRREHRLRVGGWRVLLLIDENDRSVTVLSVNARGDAYKK"
     gene            990..1949
                     /gene="pfkA"
                     /locus_tag="PK849_04325"
     CDS             990..1949
                     /gene="pfkA"
                     /locus_tag="PK849_04325"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPE65383.1"
                     /translation="MKRIAVLTSGGDSPGMNAAIRAVVRTALYHGMETIGVMKGYEGL
                     MDGECITLDRAAVGGIIHRGGTILRTARSERFKTEAGLRAALVQMENRKVDALVVIGG
                     DGSFRGAEKLCAMGLPVIGIPGTIDNDIAGTDVTIGYDTALNTALSAVQKLRDTASSH
                     DRLFIVEVMGRDAGFLALDVAVSTGAEYVVVPEIPLDIKKMCDHLHYSRKRGKTHSLI
                     ILAEGVMSALDLRNQLQDTGGYDARVTVLGYIQRGGSPSSLDTILASRMGSFAVESLL
                     EGKTGVMVGSVCGAMSLCPLEDAWKAAKELNPELLALVDKLSV"
     gene            1951..2814
                     /locus_tag="PK849_04330"
     CDS             1951..2814
                     /locus_tag="PK849_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HPE65384.1"
                     /translation="MESFGRICSVIGSDRPGRMVGGLLRPEHLSRALELLIKASSVAV
                     VTGFFVPACGAPETDGPGGAVMLGRALERCGRSATLFTDARCFSVLESCSLAVEGPAV
                     RSVETGEEILESSPSLLVFIERLGRAADGRYYNMRAEDISSVTAPLDDAAPLALTAGI
                     PVLAVGDGGNEAGMGNFRGDLSSILPEYAHCLSVTQASVTLTADVSDWGGYALAAMLS
                     LAAGEWVGPEEEEIDRMLASLVGAGAVDGVTRRGEPTVDSFNASVHRAVVRSLQEIMS
                     SAILNTKRAPL"
     gene            complement(2771..3265)
                     /locus_tag="PK849_04335"
     CDS             complement(2771..3265)
                     /locus_tag="PK849_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPE65385.1"
                     /translation="MNTTRSKEVALKGLLIAAVTLATMLSMPVPGFRLYFNFGEGIIY
                     LAALLLGPVWGAAAGGIGAAMADLLLGYPLWAPFTLLIKGTEGYIVGRIGKQHPVLGI
                     TAGAAVMIAGYSTVAGILYGWPAVPVEAATDVVQCGIGAAAALAARPVLTKALFSYSG
                     SQNS"
     gene            3506..3940
                     /locus_tag="PK849_04340"
     CDS             3506..3940
                     /locus_tag="PK849_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65386.1"
                     /translation="MSRDGKFFLLLVGFAIFSLIAVSFGAIRSKKAAGSFEIHNIVLC
                     DEVDGGIKPVGVGSAFSYGTRQLCLWFEYSRAGDDENIRVRWYYRGRPIHSQEVVLDP
                     GSGAKAFCLLLEDGSPLPKGSYSVRISLFDRVFSDTFFEISE"
     gene            4133..5500
                     /gene="rho"
                     /locus_tag="PK849_04345"
     CDS             4133..5500
                     /gene="rho"
                     /locus_tag="PK849_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPE65387.1"
                     /translation="MMSDNLIANDAADTASETAVVETALAPAETDDASKTPPKPRHTY
                     GHLAALLNPDLRRIAKELGIVGFSSLRKDDLVIAILKAQAEELNYRFGGGTLEILQEG
                     YGFLRPKGLLPSDHDVYVSASQVRRFGLRNGDVIWGLVRPPRDSEHYEALLRVEMVNF
                     SDPEAARKRPHFGAITPIFPDEKLSLETDPKEISTRLVDLFAPIGKGQRALLVSPPKA
                     GKTTLLKKIANAVTVNYPDIILMVLLIDERPEEVTDMARSVDGEIIASTFDRPADEHL
                     RVANLALEKAKRLVEVGKDVVLLLDSITRLARASNLVVPPSGRTLSGGMDPAALYFPK
                     RFFGAARNIEEGGSLTIIGTALVDTGSRMDEVIYEEFKGTGNMELHLSRKLSEQRIFP
                     AIDIVKSGTRREELLFTEDELSRLWLLRRRIVNVDESGALNLIIDKLKQSSTNKEFLL
                     TIKKG"
     gene            5586..>7099
                     /locus_tag="PK849_04350"
     CDS             5586..>7099
                     /locus_tag="PK849_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HPE65388.1"
                     /translation="MKKRNSPSPFEVRNKKAPRKKGAGFLPFAESPKGVRLGRISCFL
                     VIIVMLATAAFLTLSTADRTVGAAWGNLSDSKDSGEFILPSSGFIVVEMSNVLLDEDG
                     YELGMETDSLFALRGRRGEPGLLPLGNEVPASAKKTKDVAENSVQKLDVDTSQPKEVS
                     EASLPMTDFSWSISEQFSRGFASSSMTLGEEESASSEGAFRFPDRLVERKGQLGGNSL
                     FTTFGVSRMTEFHPRISHGFVPGESSFSTANGALFADSAVSRLVSFEASSGKGGDSSS
                     SDVSDASDFEAVVLSVGDDGKKVPLEAGEDAEDAEDVTLAQVSVLWKEHIVKAGQTLS
                     DIAVAHGVAVSDIVKANELKNANRLSEKQLLLIPNNSAAVEATLEEVLTRKARLVAAR
                     EKVLPIKITAYVVAEGDSLWSIANSQNIEIDTLFGCNDLRNPNVIRPGATLRIPNQDG
                     IFHKIKSGETLAGVAKKYEIAADRIRQANGEAALNPFPVGKEIFLPGARPEAVES"
BASE COUNT         1498 a         1850 c         2177 g         1574 t
ORIGIN      
        1 agcggtgagt aatctcacaa tggggataac aacgatagat tttatttggg atgtttcgcg
       61 cgacaatcca gaagcgctct acaaaggccg cgcggcggcc cggctcgaac tcgaccgtct
      121 gatcaagcgc atcgaaaaac tcaacgccga gaagcgggag cttgagggca agctgagcga
      181 gctgaacgcc gagcttgata ccatcaccca tcgcgtcgag gaacttcagc gccagtaagg
      241 cgccggaaac gacactacgc acgccttttc gctcgccctt ccgtcgaacg gaatggggca
      301 aaaggcttat tgaacgccga acaccgagcg aaccaaaaag ggaaggagcg cggacatccc
      361 gatccgcgct ccttcccttt tctccgtgct atactttgag ggtagaggaa attcttaagt
      421 cgagaccggg agcaggagtg tgatcgacta tgcaaaccag tctgcaagaa cgcaaacagt
      481 tgtacagaag aattcgcggg ctctcagaag acaagtacgc gaaagtagtg aaattcgttc
      541 tctccatgga aaatgaagaa cctcctcttt ccgccgagga ggaggaggat cttcgtcttg
      601 cgtatgctca aatgaagtcg ggagattatc gctctttcga tgaagcgatg aaggagcttt
      661 gggagagtga atgagtggac gctgcgaatt atggctatcg cggagaagcg gatctcaagg
      721 attccgaatc cggagaagaa gcgcattctc atggcgatcg acgaacttcg ttacgatcct
      781 tttgcgcacg atgtgatttc gctttcgggg cgtcgcgagc atcgtctgag agtgggcggg
      841 tggagagtgc ttctgctgat cgatgaaaat gaccgttccg ttacggtact ttcagtaaat
      901 gctcgcggag atgcgtacaa aaagtagcgt gctctgtggt actatttctg tgtctgtatt
      961 ttagtgtcgg gaggaacgaa cagtttatga tgaaaagaat cgcggtgttg acgagcgggg
     1021 gagactcccc gggtatgaac gcggccatcc gtgcggtggt tcgtacggcg ctctatcacg
     1081 ggatggagac cataggagtg atgaagggat acgaggggtt gatggacggc gagtgcatca
     1141 cgctcgaccg ggccgcggtc ggcggaatca tccacagggg cgggacgatc ctgcgcacgg
     1201 cgcggagcga gcggttcaag acggaagcgg ggcttcgcgc tgcgcttgtg cagatggaga
     1261 accgcaaggt ggatgcgctt gtcgtgatcg gcggcgacgg ttccttcagg ggagccgaaa
     1321 agctgtgcgc gatggggctg ccggtgatcg gcattcccgg gacaatagat aacgatatcg
     1381 ccggcaccga tgtgaccata gggtacgaca ccgcgctgaa cacggcgctc tccgcagtcc
     1441 agaagctcag ggatacggcg agcagccacg acaggctctt catcgtcgag gtcatgggcc
     1501 gcgacgcggg gttcctcgcg ctcgacgtcg ccgtttccac cggggcggag tacgtcgtcg
     1561 tccccgagat ccctctggac atcaaaaaga tgtgcgatca tctgcactac tcgaggaagc
     1621 gcgggaagac gcactctctg atcatccttg cagagggagt gatgtcggcg ctggatctgc
     1681 gcaatcagct tcaggacacc ggaggatacg acgcccgcgt gacagttctg gggtatattc
     1741 agcgaggagg aagcccgtcg tcgttggaca cgattctggc ctccagaatg ggttccttcg
     1801 cggtggagtc gctgctcgag ggaaagaccg gagtcatggt cggctcggtg tgcggcgcca
     1861 tgtcgctctg ccccctggag gacgcctgga aggccgctaa agagctgaat cccgagctgc
     1921 tggcgcttgt cgacaagctg agcgtgtgat atggagagtt tcggccgcat ttgttccgta
     1981 ataggttccg accggcccgg acgaatggtc ggagggcttc ttcgtccgga gcatctcagt
     2041 cgggctttgg agctgttgat aaaagcgtcc tccgtggctg tggtgacggg gttttttgtc
     2101 cccgcgtgcg gagctccgga aacggacggt ccgggcggag cggtgatgct cggcagggcg
     2161 ttggaacgat gcggacgaag cgcgacgctc tttacggatg cgcggtgttt ttcagttctg
     2221 gagagttgct ccctggctgt ggaggggcct gctgtccgtt ccgtcgaaac gggggaagag
     2281 attcttgaat cttcgccctc gcttcttgtt ttcattgaac ggcttgggcg ggccgccgac
     2341 gggcggtact acaacatgcg cgccgaggat atctcttctg tgactgcgcc gttggacgat
     2401 gccgcgcctc ttgcgctcac agcggggatc cccgtgcttg ccgtaggcga cggggggaat
     2461 gaagcaggca tggggaactt tcgcggggat ctttcgtcga ttcttccgga atacgcgcat
     2521 tgcctctcgg tgacgcaggc ttcggttact cttacggccg acgtgtcgga ttggggcggg
     2581 tatgcgcttg ccgccatgct ttcacttgcc gccggcgagt gggttggacc ggaagaagag
     2641 gagattgacc ggatgctcgc ttcccttgtc ggggcgggcg ccgtggacgg tgtgacccgc
     2701 aggggcgaac cgacggtgga cagtttcaac gcctccgtcc accgcgctgt cgttcgctct
     2761 ctgcaagaga ttatgagttc tgcgatcctg aatacgaaaa gagcgccttt gtaagcactg
     2821 gccgtgcggc aagggccgcc gccgcgccga taccgcactg aacgacgtcg gttgccgcct
     2881 cgacagggac tgcaggccat ccgtagagga ttcccgcgac ggtggagtac cccgcgatca
     2941 tgactgccgc tccggcggtg attccaagca ccggatgctg tttcccgatc cggccgacga
     3001 tatacccttc agttcctttg atcaggagtg taaagggcgc ccagagcggg tagccgagca
     3061 gcaggtcggc catcgcggcg ccgattcccc ctgccgccgc gccccataca ggaccgagaa
     3121 gaagcgctgc aagatagata atgccctctc cgaaattgaa gtagagccgg aatccgggta
     3181 cgggcatgct gagcattgtc gccagggtga ccgcggcgat gaggagccct ttgagggcga
     3241 cttcttttga gcgtgttgta ttcatccgat tgaacctcct tggggataca ttgctttccg
     3301 ccgggagcgg gatttctgcc gatccggaga tacacccgac atttcctgct tgcctcccca
     3361 ttctattcgt ggtagagttc tttcggaaag agccagctgg acgctgtttc catctgattc
     3421 tggactgttc aaacgatcgg ggaagtcgaa acccggcgat gttttttttc tctgcacttg
     3481 ttttttttct ggggcggtgg gtataatgtc cagggacggg aaattttttc ttcttctggt
     3541 tggtttcgct attttctccc tgatcgccgt ctcgttcggg gcgattcgtt caaagaaggc
     3601 cgcgggttct ttcgaaatcc ataatatcgt tttatgcgac gaggtggacg gcggaataaa
     3661 gcctgtcggt gtagggtctg ctttttctta tggcacgcgc caattatgtt tatggtttga
     3721 gtactccaga gccggcgacg acgagaatat ccgggtgcgc tggtattaca gggggcggcc
     3781 gattcattcg caggaggtcg tgctcgatcc aggaagcgga gccaaggcgt tctgcctgct
     3841 tcttgaggat ggctctcctc tccccaaagg atcatattcg gtccgaattt ctctgttcga
     3901 cagagtgttt tccgatacgt tcttcgaaat ttccgagtaa ctgcaactat tcacaagttc
     3961 aaaacttggt ccgttttcgc cggttcatgt gttcttttcc gcgtgaagct tttgaaacgg
     4021 ggcaattcag gaaggggatg tccaggttcg tgtttttctc tgatcattta tcccgaagtg
     4081 tgtcaaaaag ccctcctggc tttataaaag aaatacgctt aggactgcgt tcatgatgtc
     4141 cgataatctc atcgcgaacg acgcggcgga cactgcctcc gagaccgctg ttgtggaaac
     4201 ggcgctcgct cctgcggaga ctgatgacgc gtcgaagact cctccgaaac cgaggcacac
     4261 atacggccat ctggccgctc ttctcaatcc agatcttcgc aggatagcga aggagctggg
     4321 catagtcggt ttttcctccc tacgcaagga tgatcttgtc atcgccatcc tgaaggccca
     4381 agctgaagag ctcaactacc gttttggcgg aggaaccctt gaaattctgc aggaagggta
     4441 cggtttcctt cgtccgaaag ggctgctgcc cagcgatcac gacgtgtacg tttcggcgtc
     4501 gcaggttcgg cgttttggct tgcggaacgg agacgtcatc tgggggctcg tgcgtccccc
     4561 ccgtgattcg gagcactacg aggcgttgct cagagttgag atggtcaact tctccgaccc
     4621 ggaggcggca agaaaaagac cgcatttcgg cgccatcacg ccgattttcc cggatgaaaa
     4681 actttccctt gagacggatc cgaaagagat atccacgcgg cttgtcgacc tcttcgctcc
     4741 tattggaaag gggcagaggg cgctgctggt ttctcctccc aaagcgggga agaccacgct
     4801 gctcaagaaa attgccaacg cagtcacggt gaactatccg gatatcattc tgatggtgct
     4861 gcttatcgac gaacgtccgg aagaagtgac ggatatggca cgttcagtcg acggggagat
     4921 catcgcctcg accttcgacc gtccggcgga cgagcatctc agagtggcga atctcgctct
     4981 cgagaaggcc aagcgccttg tcgaggtggg caaggatgtc gttctgcttc tggattcgat
     5041 cacacgcctc gcccgcgcat ccaaccttgt tgttcctcct tccggacgaa cgctctcagg
     5101 cggtatggat cctgctgcgc tctactttcc gaagcgcttc ttcggagcgg ccaggaatat
     5161 tgaagaaggc ggaagcctca ccatcatcgg cactgccttg gtagataccg gcagccgaat
     5221 ggacgaggtg atctacgagg agtttaaagg cacgggaaat atggagcttc acctttcgag
     5281 gaagctctcc gaacagcgga tttttcccgc gatcgacatt gtgaagtcgg gaacgcgacg
     5341 cgaggagctg ctcttcaccg aagacgagct gagccgtctc tggctgctcc gccggcggat
     5401 tgtgaacgtc gacgagtcgg gcgcgctgaa tctgatcatc gataaactga aacagtcctc
     5461 cacaaataag gaatttttac tgaccatcaa aaaagggtag gggctgtctg tgcccttccc
     5521 gccgagggtc tgcggcgacg ggtggaagga cgatacttta tacaaagcag aggaggttga
     5581 ggagattgaa gaaaaggaac tctccttcgc cgtttgaggt acggaacaaa aaggcgccga
     5641 gaaaaaaagg ggcggggttt ctgcctttcg cggaatcgcc aaaaggggtg cggcttggcc
     5701 ggattagttg ttttcttgta attattgtca tgctggctac cgccgccttt ctgacgttga
     5761 gcaccgcgga tcgaactgtt ggcgcggcgt gggggaatct atcggattca aaggattcag
     5821 gagaattcat tcttccttcc tcgggcttta tcgtcgtcga aatgtcgaac gtcctgcttg
     5881 atgaggacgg ctacgagctt ggaatggaaa ccgattcgct ctttgctctc cggggcagaa
     5941 gaggagaacc cggactgctt cctctgggaa acgaagtccc cgcaagtgcg aaaaaaacaa
     6001 aggatgtcgc ggagaattcc gtgcagaagc tcgacgtcga tacgtcgcag ccgaaagaag
     6061 tatcggaagc ttctctgccg atgacggatt tcagctggtc gatttcggag cagttcagca
     6121 gagggtttgc gtcttcatca atgacgttgg gagaagagga atcggcctcc tcggagggcg
     6181 ctttccgctt ccccgaccgc ctggtagagc gaaaagggca gcttggcggc aacagtctgt
     6241 tcacaacatt cggcgtttcc cggatgacgg agtttcaccc gcggatttcc catggatttg
     6301 ttcccggcga gagctcgttc tcaactgcca acggcgccct ttttgccgac tctgccgtct
     6361 ctcgtcttgt ttccttcgaa gcctcctccg gaaaaggagg ggatagttca tcttccgatg
     6421 tttccgacgc ttcggacttc gaggctgttg tgctcagcgt aggggacgac ggtaaaaaag
     6481 tgccgctcga agcgggagaa gacgcggagg atgctgaaga tgtgacgctt gctcaggtct
     6541 ccgtactctg gaaggaacat atcgtgaagg cggggcagac gctttccgat atagcggttg
     6601 cccacggcgt tgcggtgagc gatatcgtca aggcgaacga actgaaaaat gcaaaccgtc
     6661 tctccgagaa acagcttctc ctgattccga ataacagcgc ggcggtagaa gcgacgttgg
     6721 aggaggtgct caccagaaaa gcgcgtctgg tggccgctcg ggaaaaggtc cttcccatca
     6781 aaatcaccgc gtatgttgtc gcggaaggcg acagcctctg gtcgatcgcg aactcgcaga
     6841 acattgagat tgacacgctt ttcggctgca acgatctgcg gaaccccaac gtgatacgtc
     6901 ccggggcgac cttgcgaatt ccgaatcagg acggaatctt tcataaaatc aagagcggcg
     6961 aaacgctcgc aggcgttgca aagaagtacg agatagccgc ggacagaatc aggcaggcaa
     7021 acggcgaagc tgcgctgaac cctttccccg tgggcaagga gatcttcctc cccggagccc
     7081 gtcccgaagc ggtggaatc
//
LOCUS       DAOPKU010000096         7046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11360_length_7046_cov_5.418252, whole genome shotgun sequence.
ACCESSION   DAOPKU010000096 DAOPKU010000000
VERSION     DAOPKU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11360_length_7046_cov_5.418252"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="PK849_04355"
     CDS             <1..2047
                     /locus_tag="PK849_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE65389.1"
                     /translation="DKLPETLGHLFEKLETERRILEDRSILLDNIRTQVWYLTDDHTY
                     GAVNKAHADFCGLRQEMMSFRDMFELFPREVAELHRSNAMKVFGTAEPLTVELWLPSS
                     TGELRLLSIYKSPKLREDGSVEYVVCSAEDVTERKRAGDALRESEERFRKLFSSVQSV
                     SVQGYAADGTVRYWNSASEQLYGYTADEAIGGNLLDLIIPRGMREAVRNDIRDMVEKG
                     EGRAAERLALKKKDGSRVDVLSSHVVVDYIDGRRELFCLDIDIGEQIRMEEALRAAKE
                     NAEWLGQQAEAASKAKSAFLANTSHELRTPLNGAIGFLELLADTPLNELQKEYVGYIR
                     TSAYTLLEVISEVLDISKIESDRFDLEYAQSDVFEVMRKAVDAVRGTVLRKELRLELR
                     IEEGTPRYAVVDPLRLKQVLVNLLGNAVKFTEKGFVELALQCVALDAGRCAYTFSVRD
                     TGIGIAPEVHRKLFEPFFQADASNTRKYGGVGLGITICEALLQKMESRLQLESAPGQG
                     SRFFFTLCARCDAEPEETTNGERTNLLPAKGAPLPLRTNRQAVVLLAEDQRLNRRLLR
                     ILVSKLVPGARIIEASDGEEAVRSFRSEAPDIVFMDLQMPLKDGCEAVAEIRSLERAS
                     SGGTAKAGIPIIAVTADVQKETRTFCLENGMDDFITKPVDAWSLRCVLERCLESVLL"
     gene            2386..5043
                     /locus_tag="PK849_04360"
     CDS             2386..5043
                     /locus_tag="PK849_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPE65390.1"
                     /translation="MRRLFLLLMMLLLAFVLVLTSFTSQRTTLKAFDDVERRFFIQDM
                     ARVQNRLDEELRVLRNYAADWGAWDSMYAFVEKGCKHKFDEMFDGQAMLAAGMEALLI
                     FDTGRRLLFSIFEGECAAERKLPDHVSKGLKDAVPSLMERSSDGAFAEILLLGDAAYL
                     FGVSPVLRTNRTGPSQGVLLIGRSLGKLLPEMRKSLGTKFDLLPGRAADVPDRSAGDV
                     RIVSGEGEGAAVWQGRGNEFSVRIDVPRLIRQRGLEAVESSRLWILLTGAGTLGLVLL
                     LLDRVILRRLRKLREVSERIVAGESLTLRVDEEGPEEIFALSRSFNMLLRTLHDLVMK
                     IPDPFILADADGAITLANDAAHTVLSYGTDLKGTPLSSVLVKKEDDPRAGNNRTDAEK
                     DTEAKDGHSVFEASLLRADGSLLPVEVHRRSFTFGALPLSLLLARDLKERKQFEERLA
                     RKAYFDDLTGLPNRHSLLKAINRLLNDGTRTRMFSLAVINMDHFKLVNAQVGNLNGDR
                     ILLLIAEKIKELQEPEAQLFRTSGDEFALLFPLPEGGESEEMLLMDRIRKGIATPCEV
                     GDETVFPSASIGVLVDIAEGKNASGAIDAALHAVTQARKNGIGRIARYVAPEVREYTR
                     GPNLLTLQAELQVALERHEFLPYFQPIYDLDSEKICGFETLARWMRQDGGMLAPCDFV
                     PQAEHTGFIPNIDICMMEHALRAIVRTQDMARTSRLFFSANGSPLFFRGLASMETVEF
                     LIDTTGADPKLFTIEVTESALIDNLDDVHRKLDYLKSCGVKIALDDFGTGYSSLQYIN
                     YLPFDYLKLDRAFVSQLFSSEKTEHMMRTIIGLAHNLRMDIVAEGVETTEQLSWLRAA
                     GCLKAQGFYFSRPVAWAEAEAMIRKLPEA"
     gene            complement(5153..6259)
                     /locus_tag="PK849_04365"
     CDS             complement(5153..6259)
                     /locus_tag="PK849_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65391.1"
                     /translation="MSEVILKNVTKKFSTRTLGEVTAVDDFNLTIKEGECFSLLGPSG
                     CGKTTTLRMIAGFEDLSDGEIWIGDKPVSAKKRNLYVQPEDRGLGMVFQAFAVWPHLN
                     VFENVAFPLRVRNVGRREMDSRVAEALKHTGLGGLDRTFPSDLSGGQLQRIALARAIV
                     MNPKVLLLDEPLSNLDPKLRESMRFEIKELQRRFNFTIIYVTHDQAEAMALSDRMLVM
                     DMGVIQQIDTPTNMYNNPANKFVYGFLGLSNFVDVNISDGKVYPEGGSGQPFPCAIPG
                     DFTEAAGTVASRPNEIDLTAEGGYRTVVKARIFLGDYTEYRVDVGNQEVRIQSTGDRG
                     FMVGDSVGLDFTRVQWYSRDDGISDAERERRKVI"
     gene            complement(6256..>7046)
                     /locus_tag="PK849_04370"
     CDS             complement(6256..>7046)
                     /locus_tag="PK849_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPE65392.1"
                     /translation="KFPLLGVCLASLFVIVILPLYIIFAVGGLKAYGLPLNLANMTLD
                     NYYHVLFSSRMAQDAIKNSFFLAISSAIIAMFVGTMIAYVVVKVKVKGKSLLEILALL
                     PYSVPGIVLAIGVILMWSGTFYLNLYNTIWIILIAYIARYVAFAMKSSSASLEQVHYS
                     LEEAARSCGATHLESLRDITIALIRPGMIAGFFLIFLPAMRELTTSILLYGPFSRTLG
                     VAIYAINAEGNITQSAALSGIAILIIMLGNLALRFVTKDRRSVS"
BASE COUNT         1457 a         2111 c         2142 g         1336 t
ORIGIN      
        1 ggataaactg ccggagacgc tgggacacct cttcgaaaag ctggaaacgg agcggcgaat
       61 cctcgaagac cggagcatcc tcctcgacaa catccggacg caggtatggt acctcaccga
      121 cgaccatact tacggcgccg tcaacaaggc gcacgcggac ttctgcggcc tgcggcagga
      181 gatgatgtcc ttccgcgaca tgttcgaact gttcccgaga gaggttgccg aactgcaccg
      241 gagcaacgcg atgaaggtct tcggcactgc ggagccgctt accgtcgagc tctggcttcc
      301 gtcctccact ggggagcttc gtctgctctc tatttacaaa tcgcccaaac ttcgggagga
      361 cggaagcgta gagtatgtgg tctgctccgc ggaggatgta accgagcgca aacgggccgg
      421 agatgctctc cgcgagagcg aggaacgctt caggaagctc ttttccagcg tgcagagcgt
      481 ctcggtccag gggtacgccg cggatggaac ggtgcgctat tggaacagcg cgtcggagca
      541 gctctacggg tataccgccg acgaggccat cgggggcaat cttcttgatc tgatcatacc
      601 gcgcggcatg agagaagctg tccggaacga tatccgcgac atggtggaga aaggagaagg
      661 ccgggcggca gagcgacttg ccctaaagaa gaaagacggt tcccgggtcg atgttctttc
      721 gagccatgtt gtggtggact atatcgacgg acggagggaa ctcttctgcc tggatatcga
      781 tataggagag cagatacgaa tggaggaggc tctccgggcg gccaaggaga acgccgaatg
      841 gctcgggcag caggccgaag ccgccagcaa agcgaagagc gcttttctgg cgaacaccag
      901 ccatgaactc cgtacgcctc tcaacggtgc gatcggcttt cttgaactgc tcgcggacac
      961 gcccctcaac gaactgcaga aggagtacgt cggctacata cggacgtccg cctacacgct
     1021 cctcgaagtc atcagcgaag tgctggacat ctcgaaaatc gaatccgacc ggtttgacct
     1081 tgagtacgcc cagagcgatg ttttcgaggt tatgcggaag gcagtggacg ccgtccgcgg
     1141 aactgtcttg cggaaggaac ttcgccttga actccgcatc gaagaaggga cgccgcggta
     1201 cgcagtcgtc gacccgctcc gtctgaaaca ggtgctggtg aaccttttgg gcaacgccgt
     1261 caagtttact gaaaaaggat tcgtcgagct tgcgcttcag tgcgtcgcgc ttgacgcagg
     1321 acgatgcgcc tatacgttct ccgtcaggga caccggcata ggtattgctc cggaggtcca
     1381 tcgcaaactc ttcgagccgt ttttccaggc cgacgcttcc aacaccagaa agtacggcgg
     1441 cgtcgggctc ggcatcacta tctgcgaagc gctgctgcag aagatggaga gccgcctcca
     1501 actggaaagc gcacccggcc agggaagccg cttcttcttc accctctgcg cacgatgcga
     1561 cgccgaaccg gaagaaacaa cgaacggaga gcggacgaac ctgctcccgg caaaaggggc
     1621 tcctcttccg ctgcgtacca accggcaagc cgtcgttctc ctcgccgagg atcagcggct
     1681 caaccgaagg ctgctgcgaa tcctcgtctc caagctcgtc ccgggggcgc gaatcatcga
     1741 ggcgtccgac ggagaagaag ctgtccgctc cttcagaagc gaagcgcccg acatcgtctt
     1801 tatggatctg cagatgccgc tgaaggacgg gtgcgaagcg gtagcggaaa tacgttcgct
     1861 tgaacgggcc tcctccgggg gtacggcgaa ggccggaatt ccgattattg cagtcaccgc
     1921 cgatgtccag aaggaaacgc gaacgttctg cctcgagaac ggaatggatg atttcattac
     1981 caaacctgtg gacgcgtgga gtctcagatg cgttctggag cgttgcctcg aaagtgtcct
     2041 cctctaagcg gagaaaagcg tacgtatctg ctgagagtat ccgtgcgttc cgggagcatc
     2101 gggacgcatt ttttttgcca aaataaaggt caataaaaat cagtgattac gtactctttt
     2161 ttcatgaacg ttctgggcgg atgggctgtt ttcctaggga gtatgcggcg aagttcgcgg
     2221 tttgtctcgt tgaaaaaaat tagtctttgg gatgagtaaa gtttttgttg cggagctcgc
     2281 cattcaagga gataataagc aaagtgaaat acttcatatt ctttcaggct gctgtgaccg
     2341 cactctctca gcggaagaaa tttagcgagg agatcgccca tgcccttgcg ccgactgttc
     2401 ttgctcttga tgatgctttt gctggccttt gtacttgtac taacctcttt cacctcgcaa
     2461 cggacgacgc tgaaagcgtt cgacgacgtg gagcgccgtt tcttcattca ggacatggcg
     2521 agggttcaga accgcctgga cgaggaactc cgggtgctgc gcaactatgc ggcggactgg
     2581 ggcgcatggg acagcatgta cgccttcgtg gaaaagggct gcaaacacaa gttcgacgag
     2641 atgttcgacg gacaggcgat gcttgccgcc ggcatggagg cgctcttgat tttcgacacc
     2701 gggcggcgtc tcctgttctc catcttcgag ggggagtgcg cagcggagcg gaagctcccc
     2761 gatcacgtgt ccaagggcct gaaggacgct gttccttcgc tgatggagcg ctcatctgac
     2821 ggagcgttcg ccgagatcct gctcctgggc gacgcggcct acctgttcgg agtcagcccg
     2881 gtgctgagaa cgaacaggac ggggccgtcg caaggagtgc tcctcatcgg gaggtcgctg
     2941 gggaaacttc tgcccgagat gcggaagagc ctcggcacga agttcgacct ccttccgggg
     3001 cgcgctgcgg atgttccgga ccgctcggcg ggagatgtcc ggatcgtctc cggagaaggg
     3061 gagggcgcgg ccgtatggca ggggagaggg aacgaattct ccgtccgcat cgatgtcccc
     3121 aggctcatcc gccagcgggg gctggaagct gtcgaatcgt cgcgtctctg gattctgctc
     3181 accggcgcgg ggactctcgg cctggtgctg cttctcctcg acagggtgat tctgcgccgt
     3241 ctgcggaagc ttcgcgaggt ctccgagcga atcgtcgccg gagagtcgct cacgctgcgg
     3301 gtggacgaag aggggcccga agagatcttc gcgctctcgc gctcgttcaa catgctgctg
     3361 cggacgctgc acgatctcgt gatgaaaatc cccgatcctt tcatccttgc cgacgccgac
     3421 ggcgccatta cgctggccaa cgacgcggcg cacactgtcc tgagctacgg gaccgacctt
     3481 aaaggaacgc ctctctcctc cgttctcgtc aagaaggagg atgacccccg cgccgggaac
     3541 aatagaacag atgcagaaaa ggatacggag gcgaaggacg ggcacagcgt cttcgaggcg
     3601 tcgctcctca gagcggacgg ttcgctgctt cccgtcgagg tgcaccgccg gagcttcact
     3661 ttcggcgcgc tcccactgtc gcttcttctg gcgcgcgacc tgaaggagcg caagcagttc
     3721 gaggagcgcc tcgcacggaa agcgtatttc gacgatctta ccgggctgcc caaccgtcac
     3781 tcgctcctca aagcgatcaa ccgcctgttg aacgacggaa cccgaacgcg catgttctcg
     3841 ctcgccgtca tcaatatgga ccacttcaag ctggtcaacg cccaagtcgg caacctgaac
     3901 ggcgaccgga ttctcctgct gatcgccgag aagatcaagg aacttcagga gcccgaggca
     3961 cagctcttca gaacgagcgg cgacgaattc gcgctgctct tcccccttcc cgagggcggc
     4021 gaaagcgagg agatgctcct gatggaccgt atccgcaagg gcatagcgac gccgtgcgag
     4081 gtgggggacg agaccgtctt tccctcggcc agtatcggag tgctcgtcga tattgcggag
     4141 gggaagaacg cctccggggc gatcgacgcc gcactgcacg ccgtgacgca ggccaggaag
     4201 aacggcatcg gccggatcgc ccgctacgtt gcgccggaag tcagagagta cacgcgcggt
     4261 ccgaacttgc tcacgctcca ggcggaattg caggtcgccc tggagcgtca cgagttcctt
     4321 ccctacttcc agccgatcta cgacctggat tccgagaaaa tatgcggctt cgagaccctc
     4381 gcccgctgga tgcgccaaga cggaggaatg cttgctccct gcgacttcgt cccgcaggcg
     4441 gagcataccg gtttcatccc gaacatcgat atctgcatga tggaacatgc gctccgcgcg
     4501 atcgtgcgga cacaggacat ggcgcgcacc tcgcggctct tcttctcggc gaacggatcg
     4561 ccgctcttct tccgcggact cgcctcgatg gagacggtgg agtttctcat cgacaccacc
     4621 ggcgcggacc cgaagctctt caccatcgag gtgaccgaga gcgcgctgat cgacaacctc
     4681 gacgatgtgc acagaaagct ggactacctg aagagctgcg gcgtcaagat agccctcgac
     4741 gacttcggga cgggctactc ctccctccaa tacatcaact atctgccgtt cgactacctg
     4801 aagctggaca gggcgttcgt cagccagctt ttcagctcgg agaagaccga acacatgatg
     4861 cgaacgatca tcggcctggc gcacaatctg cggatggata tcgtcgccga gggagtcgag
     4921 acgacggagc agctctcctg gctgagggcg gcggggtgtc ttaaggcgca ggggttctac
     4981 ttctcgcgcc cggtggcgtg ggccgaggcc gaggccatga tacggaagct tcccgaagcg
     5041 taaccggccg gggagccgga tgcggaaggc ctgcatcaag ctccggatgg accggcgccc
     5101 cgcgcaagcg tacaaaaaag cccccggaga gcgctccccg ggggcctgtc ttctaaatga
     5161 cctttcgtcg ttcccgttcc gcgtcgctga tgccgtcgtc gcgggagtac cactgcacac
     5221 gggtgaaatc cagcccgaca ctgtctccca ccatgaagcc gcggtcgccg gtgctctgaa
     5281 tacgcacctc ctgattcccc acgtccaccc ggtattcggt atagtcgccc aggaagatgc
     5341 gcgccttgac caccgtgcgg tagcccccct cggccgtcag gtcgatctcg ttggggcgcg
     5401 acgccacggt accggcggct tcggtgaaat cgccgggaat ggcgcacgga aacggctgcc
     5461 ccgagcctcc ttcagggtac accttcccgt cgctgatgtt cacgtcgacg aagttggaga
     5521 ggcccaggaa gccgtagacg aacttgttcg ccgggttgtt gtacatgttc gtcggcgtgt
     5581 cgatctgctg aatcacgccc atatccatca ccagcatccg gtcggagagc gccatcgcct
     5641 ccgcctgatc gtgcgtcacg tagatgatgg tgaagttgaa tcgccgttgc agctccttga
     5701 tctcgaagcg catcgactcc cgaagcttgg ggtcgagatt cgacagcggc tcgtcgagca
     5761 gcagcacctt cgggttcatg acgatggccc gggcgagcgc gatccgctgg agctgccctc
     5821 cggacaggtc cgagggaaac gtccggtcca aaccgccgag tccggtatgt ttgagggcct
     5881 ccgcgaccct ggaatccatc tccctcctcc cgacgttccg cacccgcaga gggaaggcaa
     5941 cattttcgaa gacgttcaga tgcggccaaa cggcgaacgc ctggaagacc atccccagcc
     6001 cccggtcctc cggctgcacg tagagattgc gcttcttcgc ggacaccggc ttgtcgccga
     6061 tccagatctc tccgtcgctg agatcctcga accccgcgat catccggagc gtggtcgtct
     6121 ttccacatcc cgacggcccc aacagggaga agcactcgcc ctctttgatc gtcaaattga
     6181 agtcgtcgac cgcggtcacc tctcccaaag tcctcgtgga gaatttcttc gtgacgttct
     6241 tcaggataac ctcgctcatg agacgctcct cctgtccttg gtaacaaacc ggagcgccag
     6301 gttaccgagc atgatgatca gtatggcgat accggacagc gccgccgact gggtgatgtt
     6361 cccctcggcg ttgatggcgt agatcgccac gcccagcgtg cgggagaacg gcccgtagag
     6421 cagaatcgag gtggtcagct ccctcatggc cggcagaaag atgaggaaaa atcccgcgat
     6481 catacccggc cgtatcagcg cgatggtgat gtcccggagc gattcgaggt gcgtcgcgcc
     6541 gcaagagcgg gccgcctcct caagagagta gtgcacctgc tccagcgagg cggacgaact
     6601 cttcatcgcg aacgccacgt accgcgcgat ataggcgatc aggatgatcc agatggtgtt
     6661 gtacaggttc agatagaacg tgccgctcca catcaggatc acgccgatgg cgagcacgat
     6721 ccccggcacc gaatagggaa gcagcgcgag gatctccaga agcgacttcc ccttgacctt
     6781 cacctttacc accacgtagg cgatcatggt gcccacgaac atcgcgataa tcgcggacga
     6841 aatggccaga aagaagctgt tcttgatagc gtcctgcgcc atccgcgagg agaagagcac
     6901 gtgatagtag ttatccaacg tcatgttggc gaggttgagc ggcagcccgt acgccttcag
     6961 ccctcccacc gcgaagatga tatagagcgg cagaatgacg atcacgaaca ggctggcgag
     7021 gcagacgccc agcagcggga acttgg
//
LOCUS       DAOPKU010000097         7021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11427_length_7021_cov_4.533592, whole genome shotgun sequence.
ACCESSION   DAOPKU010000097 DAOPKU010000000
VERSION     DAOPKU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11427_length_7021_cov_4.533592"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="PK849_04375"
     CDS             <1..1481
                     /locus_tag="PK849_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPE65393.1"
                     /translation="EEMEAGYEPSPKEGAKMEKALRAAPPAPLALFPPLPKKLAQPGG
                     AERQMAESRITADAVLREEVFKKAEEEGFELLQRNVAPVARTPSVLEAAKEPSKTVAR
                     GKRFEEVRAESLGGFLPRLSDDGIDLLFWSSRADGTVAGFLVDVDVLKDRIAGVLPEI
                     LTDTRILTVLDERGEPLLAPETKELPDWRRPFVAREISPLLPRWEAGAWLADPTDAAS
                     RARFAARAVWLLVGALFFVILCGGALILWMLSSEMRRARRKTTFVANVSHELKTPLTS
                     IRLFAEMLLSGRQKDEERRKEYLRTMVSEAERLSRLVENVLAFSRGGGGGESATEPLD
                     LSELTRETLSQLEPNLLKNGFACSFSTGGPAPVRGSPERLRQVLMNLLSNAEKYSGDV
                     REIGIKCGNESGYAFVEVADRGPGVPSSQKEKIFQEFFRGDNSLTASRSGAGLGLSIA
                     RSIARRHGGDVSYMPRSGGGSVFSLRIPLEEAAAGSGQGEEK"
     gene            1478..2185
                     /locus_tag="PK849_04380"
     CDS             1478..2185
                     /locus_tag="PK849_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE65394.1"
                     /translation="MNERILIAEDDFAILTGVADLLESEGYEVETACDGLTALERYRA
                     VRPDLVLLDVMMPGMNGYDVCRAIRTEDRFIPLLMLTAKSSEIDKVIGLELGADDYIV
                     KPFGMAELLARVRSALRRGALSSTARTEGAASSTLSFGDVTVNFESMTGQKNGVEFAL
                     TPKEAALLRFLTEREGKVVSRNTLLDDVWGIDCEVTTRTVDQHVVRLRQKIEDDPSRP
                     VFLCTVHGAGYRFVLEQ"
     gene            2480..4531
                     /locus_tag="PK849_04385"
     CDS             2480..4531
                     /locus_tag="PK849_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65395.1"
                     /translation="MTDASFRRLLECNPLFARLFGVEGSEAFGSTPAAEVLFPSPALR
                     ETILERFEKGFLDRFEAPFRRKDSSSFWGELSAIVSPDGSCICGILRDVTERKTNELW
                     LKEQNSSLSMLAEISADLMAELDTDSLLQKVLRYATSAMNTEHGSISFVDFTTKERYV
                     RWSQGPILSSFQGIRIPLGMGLGGEVIAAGKRKIVKDYRNFSNRLPDHDFDCIGTTVG
                     VPLRWKDEVFGYLSIYYEGEPREIEDARLEHLDQFAFLASLALHNARLYEDARLEIAE
                     RRRLEEELRKATLEAQRANEAKSDFLARMSHEIRTPLNILTGVSDLLLEREVDSELRE
                     NLSMISQAGKFLTEMVNDILDFSKIEAGMIEVERMPFSLSALLGDIAPMFVFHAERSG
                     IAFRVSADPTLPDVFTGDSLRLKQILFNLLGNAMKFTKRGFVRLAVTGVPLGEFSYEI
                     RFTVSDTGVGMMPDVLDSIFEPYHQGELSVARRFGGSGLGLPISRKLAELMGGVLSVE
                     SALGRGSDFTLSLPLEVARTPSDSKGTMEEEGTALPEKLSILVADDNTVNRTLLSKLL
                     SLPGWTVLMAADGQEALEIVRKDAPDVIILDLEMPRMDGCAAAKAIRKHEVESGKRAF
                     ILGLTGYTRSESAPLCADAGMDDCLTKPARKFTLLAGIANYFSSGGVWNTLEDDEENE
                     E"
     gene            4521..5006
                     /locus_tag="PK849_04390"
     CDS             4521..5006
                     /locus_tag="PK849_04390"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPE65396.1"
                     /translation="MKNKNVLYEQLRLVENNPLNTEALARMIADPEDLHLVWPVAEYP
                     FNHDQWRAELDPANGTRSFLVYEGERMVGHGALAPCRGDEDSVMVRFLYIAPDMRNRG
                     AGRRLLSLLEARAKDAMGARRLILKVRTYNQRAMGCYGKYGFREYSRDGTLVLMEKKV
                     L"
     gene            complement(5067..5246)
                     /locus_tag="PK849_04395"
     CDS             complement(5067..5246)
                     /locus_tag="PK849_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65397.1"
                     /translation="MEIKPLPALIFGRDFRERAINFVALVEEGAISPDDVHIFSCVET
                     ADEAWAAIKKACGIS"
     gene            5398..5778
                     /locus_tag="PK849_04400"
     CDS             5398..5778
                     /locus_tag="PK849_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE65398.1"
                     /translation="MCATVLRPDPAGEYFTEERCYILETSNSPDDPDVSIARARVAPG
                     VTTRWHRLSGTTERYYILTGTGRVEIGDLPPEDVVPGDAVIIPPLVRQRISNTGTDEL
                     VFLCVCSPRFLPEAYEDLGEERAQ"
     gene            5775..6884
                     /locus_tag="PK849_04405"
     CDS             5775..6884
                     /locus_tag="PK849_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPE65399.1"
                     /translation="MNATNDGAMETPACPVCDLVSLLYTRNVMVYLYSSLAFVPRRVI
                     EGLAASGYSPQERGMYLEIEAERFSDVIEALGRSCGLTDPEKGATVVCFTERGRPFGL
                     DSLAHVKPLSQWEDILGAGDLLDVLRNGRIVTHFQPIVDYAAKSVYACECLSRGVRSD
                     GERINPAELFGKAKKAGLIFNLDKLCRQSAIVSGASLCGSGRLFINFIPTAIYNPEHC
                     LQTTTALVEEYGVAPEKIVFEVVESESVTDLRHLQGILDYYRERGFRTALDDVGSGYS
                     SLNRLIGLRPDYVKLDIGLVRDIHKEPMKQHVFEAIETICRRSGIEMLAEGIEHAEEV
                     AFFHNAGVRLMQGYYFAKPAEVPQPVSFDDRPSSS"
BASE COUNT         1459 a         1894 c         2174 g         1494 t
ORIGIN      
        1 acgaggaaat ggaggccggt tatgaaccct ctccgaagga gggggcgaag atggagaaag
       61 cacttcgcgc ggctcctccc gcccccctcg cgctcttccc gccgcttccg aagaaacttg
      121 cacagcctgg cggcgctgag cggcagatgg cggagagccg tatcactgcg gatgctgtgc
      181 tgcgggaaga ggtcttcaag aaggccgagg aagagggatt tgagctgctg caacggaacg
      241 tcgctcccgt tgcccgaacg ccttccgtgc ttgaggccgc caaagaaccg tcgaagacgg
      301 tggccagggg gaaacgcttc gaagaggtgc gggccgaatc tttggggggc tttcttccaa
      361 ggctttcgga cgacggcatt gatctgctct tctggagttc gcgtgccgac ggaacggtcg
      421 ccggattcct tgtggatgtt gatgtgttga aagacagaat cgccggggtg ctccccgaga
      481 tactgaccga cacccgtata ctcaccgttc tggacgaacg gggcgaacct cttctcgccc
      541 cggaaaccaa agagcttccg gactggcggc gcccgttcgt cgccagggag atttcgcccc
      601 ttctgcctcg ctgggaggcc ggagcgtggc tcgccgaccc tacggacgcc gcttcccgcg
      661 cccggttcgc cgctcgggcc gtctggttgc ttgtgggagc gctctttttt gtgatactct
      721 gcggcggggc gcttattctc tggatgctct catcggagat gcgtcgtgca cgccgaaaga
      781 caacctttgt cgccaacgtc tcgcatgagc tgaaaacgcc tctcacttcc atccgcctct
      841 tcgccgagat gctgctctcg gggcggcaga aggacgaaga acgtcgaaaa gagtatcttc
      901 ggactatggt ctccgaggcg gagcgtcttt cccgtctggt agagaacgtg ctcgcgtttt
      961 cccgcggagg tggcggcgga gagtccgcga ctgagcccct tgatctttcc gaattgacgc
     1021 gcgagaccct ttcgcagctg gagccgaacc ttttgaaaaa cggttttgcc tgttcttttt
     1081 caaccggcgg accggcgcct gtgcgcggga gtccggagcg tctccggcag gtgctgatga
     1141 atctgctgtc gaatgcggag aagtactccg gcgatgtccg cgagatcggc ataaagtgcg
     1201 gaaacgagtc cgggtatgcc ttcgtggaag ttgcggacag gggaccgggg gttccctcct
     1261 cgcagaagga aaagatcttt caggagttct ttcggggaga caattcgctg acggcgtcga
     1321 gaagcggggc cggactggga ctttcgatag cgcggtcgat agcgcggcgc cacggaggcg
     1381 acgtgtcata tatgccaaga agcggcgggg gcagcgtctt ttcgctgagg ataccgcttg
     1441 aggaagcggc ggcaggctcg ggacaagggg aggagaaatg aacgaacgca ttctgatagc
     1501 ggaggacgac tttgccatcc tgaccggggt ggcggatctt ctggagagcg aagggtacga
     1561 agtggagacc gcctgcgacg ggcttacggc gctcgaaaga taccgggccg tgcggcccga
     1621 ccttgtgctc ctggatgtga tgatgcccgg aatgaacgga tatgacgtct gccgcgccat
     1681 ccgtacggaa gaccgcttca ttccgctgct gatgcttact gccaagagct cggagatcga
     1741 caaggtgatc gggctcgaac tcggggccga cgactatatc gtcaaaccgt tcggaatggc
     1801 cgagcttttg gcccgcgtcc ggtcggcgct gagaagaggc gctctgtcct caacggcgcg
     1861 gacggaggga gcagcctctt caacactctc gttcggggat gttacggtga atttcgagag
     1921 catgaccggg caaaagaacg gcgttgagtt tgcgctgact ccgaaagagg cggcgttgct
     1981 tcgttttctc acggagcgag agggaaaggt cgtctccaga aacactcttc tggacgatgt
     2041 gtgggggatc gactgcgagg tgacgacgcg gactgtcgac cagcacgtgg tccggttacg
     2101 gcaaaagatt gaggacgacc cctcgaggcc ggtctttctt tgcactgtgc acggggcggg
     2161 gtatcgattc gttctggaac aataagaaga acgtttttac atgcaaagat gttcaaagat
     2221 gattcctgcg aatcatcgaa gaggcattcc tgaatcattt ccaatgtggt attttaataa
     2281 ggatagtgga gagtatgtaa agaagggaat tgtttcaaag aatgaaacat tacctggggg
     2341 tggatgtgtg gcacgtgttc atggaatgca cgctgacgaa aactctccgt tctcttcggg
     2401 gcatttgaag aggacgactc cccgccctat cgccatatcc tggcaaaatc tgagtacgca
     2461 ggaatattac cctttcttca tgaccgacgc atctttcagg cgtcttctgg agtgcaatcc
     2521 gctctttgcc cgcctcttcg gcgtcgaggg cagcgaggct tttggaagca cgcccgcagc
     2581 cgaggtcctt ttcccttccc ccgctttacg ggagacgatt ctcgaacgtt tcgagaaggg
     2641 gtttttggac aggttcgagg cgcccttcag acgaaaagat tcttcgtcct tctggggcga
     2701 gctttcggcg atcgtttcac ccgacggcag ctgtatctgc gggattcttc gcgatgtgac
     2761 ggagcgcaaa acaaacgaac tctggctgaa ggagcagaac agttcgctct cgatgcttgc
     2821 cgagatcagc gcggatctta tggccgagct ggatacggat tcccttctcc agaaggtgtt
     2881 gcgatacgct acatcggcga tgaacaccga gcacggctcc atatcgttcg ttgatttcac
     2941 cacaaaagag cgctatgtcc gttggtcgca ggggccgatc ctctcctcgt tccaaggaat
     3001 ccggatacct ttgggcatgg ggctcggcgg cgaggtgatc gcggccggca aacgcaaaat
     3061 agtcaaagat taccggaact ttagcaacag gctgcctgat catgacttcg actgtatcgg
     3121 aacgacagtc ggggtaccgc tgcgctggaa ggacgaggtt ttcggctacc tttccattta
     3181 ctacgaagga gagccgcggg aaatcgagga tgcgcggctg gagcatctcg atcagttcgc
     3241 tttcctggct tcgttggcgt tgcataatgc aagactctac gaagatgcga ggttggagat
     3301 tgcggaacgc cgccgcctgg aggaggaact tcggaaggcg acgcttgagg cgcagcgcgc
     3361 aaacgaggcg aagagcgatt ttctggcccg gatgagccat gaaatacgaa cgccgctgaa
     3421 catcctcacc ggagtgagcg atctgctgct tgaaagagag gtcgattccg aactgcgcga
     3481 aaatctctcg atgatctccc aggccggcaa atttctcacg gagatggtga acgacattct
     3541 ggacttctca aaaatagaag cgggcatgat cgaggttgag cggatgcctt tctccctgag
     3601 cgcgcttctc ggggatattg cgccgatgtt cgtctttcat gcggagcgta gcggtatcgc
     3661 ctttcgcgtg tccgccgatc cgacgttgcc ggacgtcttc acaggggatt cgctgcgcct
     3721 gaagcagatt ctctttaatc tcttggggaa tgcgatgaaa tttacaaaaa gaggtttcgt
     3781 acgtcttgcc gtgaccggag ttcctctcgg cgaattcagt tacgagatcc ggttcaccgt
     3841 ctcggacaca ggcgtgggta tgatgccgga tgttcttgat tcgatcttcg agccgtatca
     3901 ccaaggcgag ctctccgttg cccgtcggtt cggcggttct ggcttagggc tgccgatcag
     3961 cagaaagctg gcagaactta tgggaggagt cctttcggtg gagagcgctc tcgggagagg
     4021 ttccgacttt acgctttcgc ttcccctgga ggttgcccga acgccatctg attccaaggg
     4081 aacaatggaa gaggaaggaa ccgcgcttcc ggagaagtta tcgattcttg tggccgacga
     4141 taacacggta aacaggacgc ttctctcaaa gctgctttcg cttccggggt ggaccgtctt
     4201 aatggcggcg gacggacagg aagccctgga gattgtacga aaagacgccc ccgacgtcat
     4261 tatcctggac cttgagatgc cgcgaatgga cggctgcgct gcggcgaaag ccatacggaa
     4321 gcacgaagtc gagtcgggaa aacgtgcgtt tatcctggga ttgacggggt atacccgttc
     4381 cgaaagcgcc cctctctgcg cggatgcggg tatggacgac tgccttacaa aacctgcgag
     4441 gaaatttaca ctgctggcgg gcatagcgaa ctacttttct tccggcgggg tgtggaatac
     4501 gcttgaggat gatgaagaga atgaagaata agaacgtctt gtacgaacag ctgcgacttg
     4561 tagaaaacaa cccgctgaat acggaagctc ttgcccggat gattgcggat ccggaagatc
     4621 ttcatcttgt ttggccggta gccgaatatc ctttcaacca cgaccaatgg cgggccgagt
     4681 tggaccctgc aaacgggacg cgctcattcc tcgtgtacga gggggaacga atggtcggac
     4741 acggcgcgct cgccccttgc cggggcgatg aagattccgt gatggtgcgt tttctgtata
     4801 tcgcccccga tatgcggaac agaggggcgg gaagacgctt gctctctctt ctggaagcgc
     4861 gcgcgaagga cgctatggga gcgcggcgcc ttatcctgaa agtcagaaca tacaatcagc
     4921 gcgcgatggg ctgctacggg aagtacggct tccgcgaata ctcccgcgac ggcacgctgg
     4981 tactgatgga aaaaaaagtt ctgtaaccgg ccttcgtcat cgtgagaacc gagacgtcgc
     5041 tgaaataatc ctgttttcga aaaatctcag gaaataccgc atgccttttt gatcgccgcc
     5101 caggcctcgt ccgccgtttc gacgcaggag aagatgtgta cgtcgtcggg agaaatcgcc
     5161 ccctcttcaa cgagcgcgac gaagttgatc gcgcgctccc ggaagtccct tccgaagatg
     5221 agcgctggaa gcggcttgat ctccatcttc cgggtttccg attcacgcgc gccgcaggat
     5281 gtcccgatta tagcagtgat gtcagtcgct gttcccgacg gtacttttga tgtactatta
     5341 tccgcacgat acgctccttt ttcttgggcg tcttatcctg gagaggggga atctcccgtg
     5401 tgtgcaactg tcctgcggcc agacccggcc ggtgaatact ttacggaaga gcgctgttat
     5461 atcctcgaaa cgtccaactc cccggacgat ccggacgtct ccatcgccag ggccagggtg
     5521 gcgccggggg tcacgaccag atggcacagg ctctccggca cgaccgagcg atactatatt
     5581 ctgaccggaa cggggcgtgt cgagatcggc gatcttccgc cggaggatgt tgttccgggc
     5641 gatgcggtga tcatcccgcc gctggtgcgg cagcgcattt ccaacaccgg aacggatgag
     5701 ctggtctttc tctgcgtctg ctctccgcgc ttcctcccgg aagcgtacga ggacctggga
     5761 gaggagaggg cgcagtgaac gcgacgaacg acggcgcgat ggaaactccg gcatgcccgg
     5821 tgtgcgatct cgtctctttg ctctatacga ggaacgtcat ggtatacctc tacagttcgc
     5881 tcgccttcgt tccccgaaga gtgatcgaag ggctcgccgc ctcggggtat tctccgcagg
     5941 agcgaggtat gtacctggag atagaggcgg agcgcttttc agacgtcata gaggccctcg
     6001 ggagatcctg cggcttgacc gacccggaga agggagccac ggtcgtctgt ttcaccgagc
     6061 gcggacggcc gttcggattg gactcgctgg cgcatgtaaa acctctttcg cagtgggagg
     6121 atatcctggg cgcgggagat ctgctcgacg tgctgcgcaa cgggcggatt gtcacccact
     6181 ttcagccgat cgtcgattat gcggcgaaga gcgtgtacgc ctgcgaatgt ctttcccgag
     6241 gcgtgcggag cgacggcgaa cgaatcaatc cggccgagct cttcggcaag gcgaaaaaag
     6301 ccggattgat attcaatctg gacaaactct gccggcagtc cgccatcgtt tccggagcgt
     6361 cgctctgcgg ttcaggaagg ctcttcatca acttcatccc cacggcgatc tataatccgg
     6421 aacactgtct gcagaccacg acggccctcg tcgaggagta cggcgtggcg ccggagaaga
     6481 tcgtcttcga ggtcgtcgaa tcggagagcg tcaccgatct gcgccacctt cagggcatct
     6541 tggactacta ccgtgaaaga ggattcagga cggcgctcga cgacgtcggc agcggctact
     6601 cgtcgctgaa tcgccttatc ggtctgcgcc ccgactacgt gaaactcgac atcggactcg
     6661 tccgggacat ccacaaggag ccgatgaaac agcatgtctt cgaggccatc gaaacgattt
     6721 gccgcagaag cggtatcgag atgctcgcgg aaggcatcga gcacgccgaa gaggttgcgt
     6781 tcttccacaa tgccggagtg cgcctgatgc aggggtacta cttcgcgaaa cccgcggagg
     6841 ttccgcagcc tgtctctttt gacgatcgac cttcttcatc ctgaaaaaaa tgcgcaacga
     6901 cggtcaggcg gttgcgttcc ggatgcggta tcctctgtgt cacaaggaga gggaagaatg
     6961 aaagaagcca cgcgcgcggc ctacgaagag tcgattctcc gggtacagat gcacatgcag
     7021 a
//
LOCUS       DAOPKU010000098         6905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11671_length_6905_cov_5.098686, whole genome shotgun sequence.
ACCESSION   DAOPKU010000098 DAOPKU010000000
VERSION     DAOPKU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11671_length_6905_cov_5.098686"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PK849_04410"
     CDS             complement(<1..807)
                     /locus_tag="PK849_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HPE65400.1"
                     /translation="MKTPAVAPHCRLQTVSGEGALALSERGAKAFYGAAYELVVPLID
                     GCRSADDIADALAGSVDAATVYYVLNILEQRGCVAEATPEIPPALSAFWHEAGVEPRA
                     AMEALRRSSVAVFVAGNLDSSPLETALKEAGVRTGPKELADLWVVAADDYQRDELREI
                     NREALAARRPWLLLRPTGPEAWIGPLFLPGETACFRCLLSRLERNRSAHALAKRLGGG
                     KAVPSTSVAALLSTLASAYGMAATEAARFLAGASRTLAGKLLSLDWTDWSA"
     gene            complement(878..1162)
                     /locus_tag="PK849_04415"
     CDS             complement(878..1162)
                     /locus_tag="PK849_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HPE65401.1"
                     /translation="MDRKEMRKALAKLIAKAWADARFKAKLMTDPASVLRAEGVAIPE
                     GVKIDVFENTDERIHLVIPKKPDELSDEALDIAAAGGQYSWDDNNCFITW"
     gene            complement(1242..1520)
                     /locus_tag="PK849_04420"
     CDS             complement(1242..1520)
                     /locus_tag="PK849_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HPE65402.1"
                     /translation="MDRKEMYKATGKLIAKAWVDAQFKAKLMADPASVLRAEGFSIPE
                     GMRIDVFENTDERIYLVIPKKPDQLSDEELDIAAAEGQNDVFCSCDFY"
     gene            1742..>2092
                     /locus_tag="PK849_04425"
     CDS             1742..>2092
                     /locus_tag="PK849_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65403.1"
                     /translation="MKMHEEARRAGTEKNAPAGEGEISAPLFDPESLAVSLKRGVRLH
                     ETDGRAVLVDADGVALALPLSPETRRIFLSALDEGVCTADELGDAALHAAPSTDAASL
                     YYVLAQLERGRWFRY"
     assembly_gap    2093..2192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2193..3084
                     /locus_tag="PK849_04430"
     CDS             <2193..3084
                     /locus_tag="PK849_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HPE65404.1"
                     /translation="HPALAPKDIGALLAMMRSVGALFPCDAEGRIPEDGSEQLRQWEF
                     YDLLFHSRSRQGRHGNPVGGSCPFLGEILPLPGVKRPISAERVPLHRPETGERRADFF
                     ELAEMRRSLRIQGERPLGVKELGIFLHFVGGVREILPASPEKGYWYEASLRPCAGSGA
                     IHELEYYLTVARCDGLPPGLYRYDPLAHALERLEADEKQLEKLLKGAARAMRGARCDV
                     LITLAARFQRVSWKYRSIAYSLILKDVGAVFQQMYLAASALGLAPCALGAGDSDDFAE
                     TAGLDYFSETSVGEFALSGR"
     gene            3287..4135
                     /locus_tag="PK849_04435"
     CDS             3287..4135
                     /locus_tag="PK849_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPE65405.1"
                     /translation="MLKRIISNRRARYALLFVVLLYGAALLGVRRLVYRPWANFSRTP
                     DAYGWEWEDVRFRAKDGATLHGWYIPGDSHRTVLYCHGNAGNISHGLRRADILKSLGV
                     SVFLFDYRGFGESSGRPTESGLYADTEAALDWLQEHKGLSGTDVAVFGRSLGGAAALE
                     LAVRRPVAGVLLESTFTSLADVARAFVPYLPAELFVFNAFDNMRKIERINVPVLIMAS
                     IDDEITPFEHGQRLFEEAAEPKFFLTMNGRHRSHFYRTEQEYVDAIRDFLEFVFGAEQ
                     PDLQES"
     gene            complement(4164..5702)
                     /locus_tag="PK849_04440"
     CDS             complement(4164..5702)
                     /locus_tag="PK849_04440"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE65406.1"
                     /translation="MNSLLITGGTLWTGRTFVRGYALHIEGERIAAVGPEEEVRAGAP
                     SECRELRLSGEAVLPGFTDAHLHLTTWAKQKTLLDLGGATSLAEVLELVREEARTTPS
                     DRWIRGWNYNETLWPEGRSVTKDDLDALGIPHPVLLQRVCTHVNVANSRALALVGLES
                     PDGILEERDAIPALRAMERSVFSREGLREALKSACFELASVGVTCVHPCGADDYGMEE
                     DLSLYDELRRRGELPVRVFTYHDALPYPVMPSGFGGEWIRYQGLKIFLDGSLGGRTAA
                     LTAPYADEPAQTGRLNWTDEQVLGMLRAARGKGIQTLLHAIGDRALDQALDCIGKVDE
                     EFGPAALKDRVNHLIVCRPEQRRRLAELGLFCDIQPSFIPSDIAMAPLRLGPERMEWT
                     YRWRSILNECGPLSASSDAPVESVNPMHSLWALVERRGKDGEAPLSPEERLTVEEALP
                     LFTLNPYKAVGRSDAGRLEAGCLADVVVLDRDLHGLSGDDLYSVGVRCTIAGGRATHE
                     EMEA"
     gene            5834..6742
                     /locus_tag="PK849_04445"
     CDS             5834..6742
                     /locus_tag="PK849_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPE65407.1"
                     /translation="MKRVLFSALAVLLLFASAAAAGPVSVFVSILPQKYFVEQIGGER
                     VDVSAMVEKGRDPHVFEPLPAQMEGLSKAKAYFAVGLPFEESLLPRAASLNPGLKIYR
                     IDEGIDKIAGGHEHHEEHEDEPEQNDGKHDHHVHAPHREGADPHVWNSPREAILMAGK
                     ILEGLIEIDPDGREEYARRHADFVERLKTLDAEFAGMFRGRQGATFLVFHPAWAYFAR
                     SYGLVEVAIETEGKEPKAADLANIISEARERGVKAIFISPQFSRRSAEIVAQAIGASV
                     TTADPLAAEWEENLRAVAKAIAEAAK"
BASE COUNT         1246 a         2122 c         2194 g         1243 t
ORIGIN      
        1 agcgctccag tcggtccagt cgaggctcag cagcttcccc gcaagcgtgc gggacgctcc
       61 ggcgaggaag cgcgccgcct ccgtcgccgc catgccgtac gccgaggcca gcgtcgacag
      121 gagagccgct acggaggtcg agggaactgc cttcccgcct ccgagccttt tcgccagagc
      181 gtgcgcactg cggttgcgtt cgagtctcga cagaagacag cggaagcagg cggtctctcc
      241 gggcaggaag agcgggccaa tccacgcctc cggccccgtg ggacggagca gcagccaggg
      301 gcgacgcgcc gcgagcgcct ccctgttgat ctcgcgcagc tcgtcccgct ggtagtcgtc
      361 cgcggcgacg acccacaagt ccgccagctc tttcggcccc gtccgtacgc ccgcctcttt
      421 caacgccgtc tccagcggcg aactgtccag attccccgcg acaaacacgg cgacggaact
      481 cctccgaagc gcctccatcg cggcgcgcgg ttcgacgccc gcttcgtgcc agaaagcgga
      541 cagggcggga ggtatttccg gagttgcctc cgcgacgcac ccccgctgct ccaggatgtt
      601 caagacgtag tagacagtcg cggcgtcaac gctgccggcg agcgcgtccg cgatgtcgtc
      661 ggcgctgcgg catccgtcga tcagcggaac aacaagttcg tatgcggcgc cgtaaaaggc
      721 tttcgccccc ctctcggaga gcgcgagcgc tccttcgccg gaaaccgttt gcaggcggca
      781 gtgcggcgcg accgccgggg tcttcatcgt gcgctttccg aacaatacgg gatgaacaat
      841 ctcccgtacc ggatgggagc cgcgccggaa gtttccgtta ccacgttatg aagcaattgt
      901 tgtcatccca tgagtactgc ccgcctgcgg cggcgatgtc gagcgcctcg tccgagagtt
      961 cgtcgggttt tttcggaatc acaaggtgta tgcgctcgtc agtattttcg aagacgtcga
     1021 tttttacccc ttccggaatc gccactccct cggccctcaa caccgacgcg ggatcggtca
     1081 tcagcttcgc cttgaaccga gcgtccgccc acgccttggc gatcagcttc gccaatgctt
     1141 tgcgcatctc ttttctgtcc ataccgtacc cctcccgtta aataaagtag cgcttcggcg
     1201 cgcgtccgcc gctcatgggc ggacgggcat accatgaagg ctcagtagaa gtcgcacgag
     1261 cagaagacgt cgttctgccc ctcggccgcg gcgatatcca actcctcgtc agagagttgg
     1321 tcgggcttct tcgggataac aaggtatatg cgctcgtccg tgttttcgaa gacgtcgatc
     1381 cgcattccct ccggaatcga gaatccttcg gcccgcagaa ccgacgcggg atcggccatc
     1441 agcttcgcct tgaactgcgc gtcaacccac gccttggcga tcagctttcc cgttgccttg
     1501 tacatctctt ttctgtccat tttcgttcac cctccacact gaaattttag aaatccgagc
     1561 tattctgaat tataaactct gacatagtgt gagagtcaag gctgctttat gctttttttc
     1621 gaaaaaatga taaagtggtg tccaaagaaa gaccgcttcg atgaacacgc tgcctctgca
     1681 ttggcgagcg agcggaacgg acagacgaaa agaaaaaagg gcaagagaaa agggtgaaat
     1741 aatgaaaatg cacgaagaag cgcgccgcgc aggcactgaa aaaaatgctc ccgccgggga
     1801 gggagaaata tccgccccgc tcttcgaccc ggaatctctc gcagtctctt tgaagagagg
     1861 cgtccgtctg cacgaaacgg acggacgcgc cgtacttgtc gacgcggacg gcgttgcgct
     1921 cgcccttccg ctttctcccg aaacgcggcg gattttcttg tcggcgctgg acgagggagt
     1981 atgtaccgcg gacgagctgg gggacgcggc gcttcacgcc gcgccttcaa ccgacgcagc
     2041 ctctctgtac tacgttctgg cgcagctcga acggggacgg tggttccggt acnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcatcccg cgcttgcccc aaaggatatc
     2221 ggagcgctgc tcgcgatgat gcgcagcgtc ggcgcgctct tcccgtgcga cgcggaggga
     2281 cggattccgg aggacgggag cgaacagctg cgtcagtggg agttttacga tcttctcttc
     2341 catagccgca gcaggcaggg aagacacggc aatcccgtag gaggatcctg tccgttcctc
     2401 ggggaaattc tcccgctgcc gggagtgaag cgtccgatat cggcggagcg cgttcctctt
     2461 caccgcccgg agacggggga gaggcgcgcg gatttcttcg aactcgcgga gatgcgccgt
     2521 tcgctgagga tccaggggga gcgaccgctc ggcgtaaaag aactggggat tttcctgcac
     2581 ttcgtgggag gcgttcggga aatactgccc gcctccccgg agaaggggta ttggtacgag
     2641 gcttcgctgc gcccttgcgc gggaagcggg gcgatccacg aactggagta ctacctgacg
     2701 gtcgccaggt gcgatgggct tccgccgggg ctgtaccggt acgatccgct cgcccatgcg
     2761 ctcgaacgac tcgaagcgga cgaaaaacag ctggaaaagc tcttgaaggg cgcggcccgc
     2821 gccatgaggg gagcgcgctg cgacgtgctg atcactctgg cggcacgatt tcagcgcgtt
     2881 tcgtggaagt atcgctccat cgcctactcg cttatcttga aagacgtcgg cgctgtcttc
     2941 cagcagatgt acctcgcggc cagcgcgctg ggcctcgccc cctgcgcgct gggcgcgggc
     3001 gactcggacg acttcgccga aacggccggc ctcgattatt tctcggagac gtccgtcggg
     3061 gagttcgcgc tctcgggacg gtgaacgaag ggcgcaaaga agcgccggtt ccgcaaaacg
     3121 gccgaggaac gcgcgcgcaa aagcgcgttg attcgaccgc agccttccga tagaatgctt
     3181 tatggaacac tcaggggcgt tcttcgggaa aggcgttccg gcccgggtcc cgaaaaccgt
     3241 cgcctcatcg gagggttcgc ccatatgacc cgaaaggaaa gaatacatgc tgaaacgaat
     3301 catttcaaac cggcgcgcgc gctacgcgct cctcttcgtc gtgctgctct acggcgcggc
     3361 ccttctgggc gtgcggcgcc tcgtctaccg tccgtgggcg aacttttccc gcactccgga
     3421 cgcgtacggc tgggagtggg aggacgtccg gttccgggcg aaggacggcg cgacgctcca
     3481 cggatggtac attcccggcg attcgcacag aacggtgctt tactgccacg gaaacgcggg
     3541 gaacatctcc cacgggctgc ggcgcgcgga tattctgaag tcgctgggcg tgtcggtctt
     3601 cctgttcgac tacagggggt tcggcgaaag ctccggcaga ccgacggaga gcgggctcta
     3661 cgccgatacg gaggccgctc ttgactggct gcaggagcat aaggggcttt cgggaacgga
     3721 tgtagcggtc ttcgggcgct cgctgggcgg cgctgcggcg ctggagctgg cggtgcgccg
     3781 cccggttgcg ggcgtgctcc tcgaatccac ctttacgagc ctcgcggacg tggccagggc
     3841 cttcgtgccc taccttcccg ccgaactgtt cgtcttcaat gcgttcgaca acatgcggaa
     3901 gatagaacgc atcaacgtgc ccgtcctgat aatggcgagc atcgacgacg agatcacccc
     3961 gttcgaacac ggacagcgcc tcttcgaaga ggccgcggag cccaagttct ttttaacgat
     4021 gaacgggcgg caccggtcgc acttctaccg cacggaacag gaatacgtcg acgcgatccg
     4081 ggactttctg gagttcgtct tcggagcgga gcaacccgac cttcaagaaa gctgaacggc
     4141 gtcccgaccg tgagtgcggg tatctacgcc tccatttctt cgtgcgtcgc gcgccctccc
     4201 gcgatggtgc agcgtacgcc gacggagtat aggtcgtccc ccgagaggcc gtgcagatcc
     4261 ctgtcgagca caacgacgtc ggccagacac cccgcttcga ggcgtccggc gtcgcttctg
     4321 ccgacggcct tgtacgggtt caacgtgaag agcggaagcg cctcttcgac ggtcagccgc
     4381 tcttcgggcg agagcggcgc ttcgccgtcc ttcccgcgcc gttcgacgag cgcccagagc
     4441 gaatgcatcg ggttgaccga ctcgacgggg gcgtcgctgg aggcggagag cggcccgcac
     4501 tcgttgagga tggagcgcca ccggtaggtc cactccatcc gttccgggcc gaggcgcagc
     4561 ggcgccatgg cgatgtcgct ggggatgaac gagggctgga tgtcgcagaa gagccccagt
     4621 tcggcgagac ggcggcgctg ctccggacgg cagacgatga ggtggttgac cctgtccttc
     4681 agcgcggcgg ggccgaactc ttcgtctacc tttccgatgc agtcgagcgc ctggtcgagc
     4741 gcccggtcgc cgatggcgtg gaggagcgtc tgaatcccct tcccgcgcgc ggcgcggagc
     4801 atcccgagga cctgctcgtc cgtccagttc agccgtccgg tctgggccgg ttcgtccgcg
     4861 tacggggcgg tgagcgcggc ggtgcgcccg ccgaggctgc cgtcgaggaa gatcttgagc
     4921 ccctggtagc ggatccactc cccgccgaag cccgagggca taacggggta ggggagggcg
     4981 tcgtggtagg tgaagacgcg caccggcagc tcccctctgc ggcggagttc gtcgtagagc
     5041 gagaggtcct cttccatgcc gtagtcgtcc gcgccgcacg ggtggacgca ggtgacgccc
     5101 acggaggcca gctcgaagca ggcggatttg agcgcctccc ggagtccctc gcgggagaag
     5161 acgctccgct ccatcgcgcg gagggcgggg atggcgtcgc gctcttcgag gatgccgtcg
     5221 ggactttcaa gcccgacgag agcaagagcc cggctgttcg cgacgttgac gtgcgtgcag
     5281 acgcgctgga gcagcacggg gtgcgggatg ccgagagcgt ccagatcgtc cttcgtcacg
     5341 gagcgcccct ccggccagag cgtctcgttg tagttccacc cgcggatcca acggtcggac
     5401 ggagtggtcc gcgcctcttc gcggaccagt tcgaggacct ccgcgagcga cgtcgcgccc
     5461 ccgagatcga gcagcgtctt ctgtttggcc caggtggtca ggtggaggtg cgcgtcggtg
     5521 aagccgggaa gcaccgcctc acccgacaga cgaagctcgc ggcactccga cggcgcgccc
     5581 gcgcggacct cctcctccgg accgaccgcg gcgattcgtt ctccttctat atgcagcgcg
     5641 tatccccgga cgaatgttct tcccgtccag agggttcctc cggtgatgag cagactgttc
     5701 atggacgccc tccttacaca aaagattccg gtgttattat agcggatacg aacagcgcgg
     5761 cgcacgctga aagcatgtgg agaaaggggc aatcgtgata gaatcatgcc gcaatcttcg
     5821 ggaggtgttt tttatgaaaa gagttctttt ttcagcgctg gcagtgctgc tgctttttgc
     5881 gtcggccgct gcggcggggc cggtttccgt cttcgtctcc attctgccgc agaagtactt
     5941 cgtcgagcag atcggcggcg agcgcgtcga cgtctcggca atggtggaga aggggcgcga
     6001 tccgcacgtt ttcgagccgc ttcccgcgca gatggagggg ctgtcgaagg cgaaggcgta
     6061 cttcgcggtc gggcttccct tcgaggaaag cctgcttccc agggcggcga gcctgaaccc
     6121 cgggctgaag atctaccgca tcgacgaggg gatcgacaag atcgcgggcg gccacgaaca
     6181 tcatgaggaa catgaagacg agccggaaca gaatgacgga aagcacgatc accacgtaca
     6241 tgccccccac cgcgagggcg ccgacccgca cgtctggaac agcccccgtg aagcgatcct
     6301 catggcgggg aagatcctcg aaggactgat cgagatcgac cccgacggca gggaggagta
     6361 tgcgcgacgc cacgccgact tcgtggagcg ccttaaaacg ctggacgccg aattcgccgg
     6421 gatgttccgc ggcaggcagg gagcgacctt tctcgtcttc caccccgcat gggcttactt
     6481 cgccagaagc tacggccttg tcgaggtggc catcgagacg gagggcaagg agcccaaggc
     6541 agccgatctg gcgaacatca tctcggaggc cagggaacgg ggcgtcaagg cgatcttcat
     6601 ctcgccgcag ttttcgagaa gaagcgccga aatcgtcgcg caggccatcg gcgcatccgt
     6661 cacaaccgcc gacccgctcg ccgcggagtg ggaggaaaac ctccgagcag tggcgaaggc
     6721 gatagcggag gcggcgaaat aagaacacta cgctcgtttc gaatacgcac agccggaggg
     6781 ggactcggct tcccttccgg ctgtgcgtat tcgggacttt tacaaattga ctgaatggat
     6841 ttttacccgt atgatttcat tattcttcgt cttgcgcagt tgaaaggagc gctccatgaa
     6901 cgaca
//
LOCUS       DAOPKU010000099         6898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11684_length_6898_cov_4.349408, whole genome shotgun sequence.
ACCESSION   DAOPKU010000099 DAOPKU010000000
VERSION     DAOPKU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11684_length_6898_cov_4.349408"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PK849_04450"
     CDS             <1..336
                     /locus_tag="PK849_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPE65408.1"
                     /translation="HNYPVWLSFKGGKGVATSYGVAFFLYPHLSFFVAPAGGLVWLLV
                     LKAKGYVSLASMTSLCCLPFFALVLAFPLPYVLVLGALALLAIFRHKDNIKRLREGKE
                     SRFGKHAKS"
     gene            765..1244
                     /locus_tag="PK849_04455"
     CDS             765..1244
                     /locus_tag="PK849_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HPE65409.1"
                     /translation="MASLTRVIENYINNLFSEMDEQTVSLRRKELAETFGCVPSQINY
                     VLRSRFTPERGFIVESQRGGHGYIRIVRICYDIPEEKVNHIDEIVGDSITEQEARRIL
                     TSLQERGMIDSRERLLIEVALRHTDELGSTEFDISPYKRGALAAELLKRLLRSLALA"
     gene            1293..3797
                     /locus_tag="PK849_04460"
     CDS             1293..3797
                     /locus_tag="PK849_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPE65410.1"
                     /translation="MWHFFTERGRKVVQLAHREALRLGHDVVGTEHVLLAILSEGEGA
                     GVQTLEALGVNLAELRSRIESTVGKSHPILKPVDLPLSPRAKRVFDLSVREARNMGVN
                     YVGTEHILLGILAEGESAAARVLLGLGIDHATLAKEIMRFLEGENGSGKGTENSADNR
                     RKNGRSKTPTLDQLALDLSERSRNGELDPVIGRSKEIRRIVQILSRRTKNNPVLIGEP
                     GVGKTAIVEGLAQKIMEGDIPELLKDKKVMQLNMGNLVAGTKYRGEFEERMRKLVKEL
                     SEGRDVILFIDEIHTIVGAGGAEGAVDAANILKPSLSRGEFQVIGATTLEEYRKHIEK
                     DAALERRFQPVFVQEPSVDDSIRILEGLRDRYESHHKARIQDDALEAAARLSARYIMD
                     RRLPDKGIDLIDEAAARARIKTMEPPEELKTLEQRLEEIRKEKEAAVDGQEFERAAEL
                     RDAERLLFEKLEEKRKNWRSGRNHSQPVITGEDIADVVAEWTGIPVFQITGEESLRLL
                     GMEREIEKRLVGQSEAVASVARAIRRARSGLKDPKRPIGSFLLLGPTGVGKTELARRL
                     ASFLFGTDDSMIRLDMSEFMEKHEVSKLIGAPPGYVGYEDGGKLTEAVRRRPYSVVLF
                     DEIEKAHPDIFNLLLQILEDGRLTDGHGRTVDFRNTVILMTSNVGARDAAKGSPLGFA
                     GSGNDDPFDWSRLKSTILDEVNRTFRPEFINRVDESIVFRPLGRESVGAIVDIMFADV
                     CSRLSGQEVTLTVGEEVKNLVCEKGYQPKFGARPLRRTIQALVEDPLADYLLENGASK
                     GLHVEAFVSEGHVTFSVVPAPVVESSEDRARETVHQ"
     gene            3837..4664
                     /locus_tag="PK849_04465"
     CDS             3837..4664
                     /locus_tag="PK849_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPE65411.1"
                     /translation="MDGSSLWFYFLLFFVVFPYLKQQSLRWRRFTALKNCEKRRRSRI
                     ITLIHRQESMGFWGMFSRNFINIEDSEEVLRAIRLTSDDTPIDLIIHTPGGLLLAAEQ
                     IARALKHHPAKVTVIVPHYAMSGGTLIALAADEILMDRHAVLGPVDPQIGQYPAASIL
                     KAVEEKPVSEVDDNTLILADISRKAIRQTKNFVFELLSERMGEEKGMELAEALTEGRW
                     THDYPIGVDEATALGLPVVLNDYDSFCNIMGLYHQSVTERPSVQYIPVPYPGRPSGN"
     gene            4682..5545
                     /locus_tag="PK849_04470"
     CDS             4682..5545
                     /locus_tag="PK849_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPE65412.1"
                     /translation="MSKSEKWVFSLLFLSLVLIHPLAAAAAPGNEIVLTVDDEQVSVD
                     EILYLLALQSGAGEAAAPLLAAQMTPTEREAFLEQVSRAILFSKGAIIRGLHLDPGVS
                     ARIRWSRINTLAEAYVGSMASSLSFEEKTLRAHFEKNKQKYRQNESVRVRHVFTTEEE
                     NARTALLRILSGEVFAEVAASLSVDYATAETGGDMGWISRGSLPESFEEAVFSAPLQS
                     VLGPVQTKYGYHVFEVLERRRARALSFEEVRDAVRAELVEHVMATEAASLKARFPVLL
                     VPSALEKSFAR"
     gene            5726..>6898
                     /locus_tag="PK849_04475"
     CDS             5726..>6898
                     /locus_tag="PK849_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPE65413.1"
                     /translation="MEQTSAKTPTFGQALFVLLVDAAIISYGVLDIHFESGAVFGLAL
                     SAHIPLFLAAVFTAIMGAIFVGKPWREVEEGMINGITVALQAVLILLAVGGLVGAWIL
                     SGVVPTLIFYGLNLLSPQYFLLASLLICAIVSLSTGSSWSTSGTVGIALMGIGAGLGV
                     PAPVTAGFVISGAYFGDKISPLSDTTNLAPAVAGSELFTHIRAMLWTTGPTLIIVAGI
                     AYYMGSGYTGGEMDASRISIIQQLMSAEFSISMYGFLPPLLVLVLAAMKIPAIPGIVA
                     GMLLASVMALFQGISIPDVLSALHSGYSPSMIAEVAEAGDISSVNELLKGAAVFSADA
                     FETVKEVAGMLNGLLERGGLTGMLETVALILVALVLGGIMETCGFLEVLLARLMQSV"
BASE COUNT         1566 a         1689 c         1924 g         1719 t
ORIGIN      
        1 cacaactacc ctgtttggct ctctttcaaa ggaggaaagg gagtcgcgac atcttatggc
       61 gtcgcgttct tcttgtatcc tcatctttct ttctttgttg ccccagcggg cggcctggta
      121 tggctgctcg ttctgaaagc aaaaggttac gtctctttgg cttcaatgac ctctctctgt
      181 tgcctgcctt tttttgcgct cgttcttgct ttccctcttc cgtatgtttt ggtgttgggc
      241 gcgcttgcct tgctggcaat tttccgccac aaagataata tcaagcgatt acgagaagga
      301 aaggaaagcc gcttcggaaa acacgcaaaa tcctgaagaa ctgaaagaac tcgaatccgc
      361 agacggtagg tccgtctccg gagtactctg cggcagattg cgcagagtat cgtcaatgct
      421 atctgctcaa gcgggcaaga agttgttttc tgttcgccgg ttgtgctctt ctgtctttaa
      481 aacctgtggt agaggcagac acccctcctt attgactaat actgaccaac atcgtagaat
      541 agcctataag agatgcgttc aatctgagta cttgtttgca gattgaacca tatctttgag
      601 ggaaatacgt ttattgtttc actctttctg cttcaagtgg agaaacgagg atctgttttc
      661 cgccgagcgg caaggtatgc gtgggataga ttgtccggag cagattggtt atacagttca
      721 gtaacgtgct gtttgtatgg aatttgcagt gtggagggaa taaaatggca agcctgacac
      781 gggtcatcga aaactacata aataaccttt tctccgaaat ggatgaacag acagtttcac
      841 ttcggagaaa ggagcttgcg gagactttcg ggtgtgtccc gagccagatc aactatgtcc
      901 tccgaagccg tttcactccc gagcgcggtt ttattgtgga gagtcagaga ggcggtcacg
      961 ggtatattcg aatagtccgt atatgctacg atataccgga agagaaagta aatcatattg
     1021 atgaaatagt aggagattcc atcaccgagc aggaagcccg gcgtatcctt acgtctcttc
     1081 aggagcgcgg gatgattgat tcgagagaac gtcttctcat tgaggtggcg ttgcgacaca
     1141 ctgacgaact gggtagcacc gagtttgata tctctcccta taagagaggc gccctggcgg
     1201 cggaactgtt gaagcgtttg cttcgcagtc tcgctctggc ctgatggcag gatgagaacg
     1261 gcccttcggg gccatgtaaa gggtggtttg gaatgtggca ttttttcacg gaacgcggca
     1321 ggaaagtggt gcaactagcg cacagagagg ctctccgtct tggacatgac gttgtgggaa
     1381 cagaacacgt cttgcttgct attctctccg aaggggaagg ggcgggtgtt caaacccttg
     1441 aagctcttgg agtgaatctt gccgagctgc gctctcgtat tgaaagtact gtaggcaaat
     1501 ctcatccgat tttaaaacct gtggatttgc ctttgagccc gcgggcaaag agggtttttg
     1561 atctttccgt tcgcgaagcg cggaatatgg gcgtgaacta tgtcggcacg gagcatatac
     1621 ttctcggcat cctcgccgaa ggagagagcg ccgccgcccg tgtccttctg gggctcggca
     1681 tagatcacgc tactctggca aaagagatca tgcgatttct cgaaggggaa aacggttcgg
     1741 gtaaagggac ggaaaactct gccgacaacc gtcggaagaa cggacgttcg aagacgccta
     1801 cactggacca gctcgcgttg gatctcagtg agcggagcag aaacggtgaa ctcgatccgg
     1861 ttattggaag aagcaaagaa attcgtagaa tcgtccagat cctctcccga agaacaaaga
     1921 acaatccggt gcttatcgga gaacccgggg taggaaagac cgcaattgtc gagggactgg
     1981 ctcagaaaat tatggaggga gatatccccg aacttctgaa ggataagaag gttatgcagc
     2041 tcaacatggg gaatcttgtt gcaggcacga aatacagggg agagttcgag gagcgcatgc
     2101 gtaaactggt gaaggaactc tccgaaggac gcgacgtcat tctcttcatc gacgaaatcc
     2161 acactattgt cggcgccggg ggcgcagaag gcgcggtgga tgcggccaac attttgaagc
     2221 cgagtctttc caggggggaa ttccaggtta tcggcgccac taccctggaa gagtacagga
     2281 agcatatcga gaaggacgct gcgctcgaaa gacgcttcca gccggtattt gttcaggagc
     2341 cgtccgtgga tgactccatc cgtattctgg agggactcag agaccggtat gaatcgcacc
     2401 acaaggccag aatacaggat gacgccctgg aagcggccgc ccggctttct gctcgctata
     2461 ttatggacag acgtcttccc gataaaggaa tcgatcttat cgacgaagcc gccgccagag
     2521 cgaggatcaa gacgatggag ccccccgaag aactaaagac gctcgaacag cgtctggaag
     2581 aaattcgtaa agagaaggaa gccgccgtgg acgggcagga atttgaaagg gctgcggaac
     2641 tgcgcgacgc cgagcgtctg cttttcgaaa agctggaaga gaaacggaaa aactggaggt
     2701 cggggagaaa tcattctcag ccagtgatca caggtgaaga tattgcggac gtagttgcag
     2761 aatggaccgg catcccggtg tttcagatca caggggaaga gtctttgcgt cttcttggaa
     2821 tggaacgcga gatagaaaag aggctcgtag gacagagcga agctgtcgcg tcggttgcga
     2881 gggctatcag gcgcgccaga agcggtctga aggacccgaa gcggcccatt ggaagcttcc
     2941 ttctgcttgg gcctacagga gtgggcaaga ctgagctggc gagacgcctg gcatccttcc
     3001 tcttcgggac cgacgattca atgattcgtc tcgatatgag cgagttcatg gagaaacatg
     3061 aagtctcaaa gcttattggc gctcctcccg ggtacgttgg gtacgaagat ggggggaaac
     3121 tcacggaagc ggtgcggcgc cgcccctatt ccgtggtgct tttcgatgaa attgaaaaag
     3181 cgcatcccga catattcaac ctgcttctcc agatactgga agacggccga ttgactgacg
     3241 ggcacgggcg gacagtggat ttccgcaata ccgtaatcct gatgaccagt aacgtcggcg
     3301 ccagagatgc cgcgaagggg tctcctcttg gattcgccgg atcgggaaac gacgatccgt
     3361 tcgactggag ccgtcttaaa agcactatcc ttgacgaagt gaaccgaacc ttccgtcccg
     3421 aattcataaa tcgtgtcgac gagagtattg tcttccgccc cttaggaaga gagagcgtcg
     3481 gcgcaattgt tgatattatg tttgcagacg tctgttcgcg attgtcgggc caggaggtca
     3541 cgctgacagt gggagaagaa gtgaagaact tagtgtgcga gaaagggtat cagccaaagt
     3601 tcggggcccg gcctctcaga aggacgatac aagcgcttgt cgaagacccc ttggctgatt
     3661 atcttcttga aaacggggct tcaaaggggt tgcacgtcga ggcgtttgtt tccgagggac
     3721 atgtcacttt cagcgtcgtc cctgcgcccg ttgtagagtc ttcggaggat cgtgcgcgtg
     3781 aaactgtgca tcagtagtta ttcgcttgtg ctcgttgttt ctggaaggag gctgtcatgg
     3841 acggatcatc gctttggttt tacttcctgc tcttttttgt agtctttcct tacttaaagc
     3901 agcagtcgct tcggtggagg cgttttactg cgctcaaaaa ctgtgaaaag agacgccgtt
     3961 cacggattat aactttgatt caccgacagg agagcatggg attctggggg atgttctccc
     4021 ggaactttat caatattgag gactccgagg aggttcttcg agctattcgc ttgacctcgg
     4081 acgatacccc catagacctg atcatacata cgccaggcgg tctccttctc gcagcggaac
     4141 agattgcacg cgctttgaag catcatccgg caaaggttac tgttatcgtc ccgcattacg
     4201 ccatgtcagg cgggacgctt atcgctctcg cagccgacga aatcctgatg gatcgccatg
     4261 cggttctcgg tcctgtggat cctcagatag ggcagtaccc ggcggcctct atactgaaag
     4321 cagtagaaga aaaacccgtc agcgaggttg atgacaatac gctgatactt gccgatatct
     4381 cccgaaaggc gatccgccag acgaagaact tcgtttttga gctcctttcc gaaagaatgg
     4441 gtgaagagaa ggggatggag ctcgcggagg ctctcacgga aggccgatgg actcatgact
     4501 acccgatagg agtagacgaa gcgaccgctc tcgggcttcc ggttgtgctc aatgattatg
     4561 actccttctg caacatcatg gggttgtacc atcagtccgt taccgagagg ccttccgtcc
     4621 aatatattcc cgtcccctac ccgggacgcc cttccggaaa ttgaaaggcg ggtgtgttcg
     4681 aatgagcaag tcggaaaagt gggttttcag tctccttttt ctttctctcg tactaattca
     4741 tccgcttgcc gcagcggcag cgccggggaa tgaaattgtc cttactgttg atgacgagca
     4801 agtttctgtt gatgaaatac tctatctttt agctcttcag tcaggggccg gcgaagccgc
     4861 tgcccctctg cttgcagcgc agatgactcc aaccgagaga gaggcgtttc ttgaacaggt
     4921 ttcgcgggcg attctcttct cgaagggggc aattatccga ggattgcatc ttgatcctgg
     4981 agtttcggcc cgtatccgtt ggagccgcat caatacgctt gccgaggcgt atgtcggcag
     5041 tatggcctct tcgctctctt ttgaagagaa aaccctgaga gctcattttg aaaagaataa
     5101 acagaaatac cgacagaatg aatcagtccg ggtcaggcat gtattcacca cagaagagga
     5161 gaatgcgcga acagcgcttc tccgcattct ctcgggagaa gtttttgccg aagtcgctgc
     5221 atcgctcagc gttgattacg ccacggccga aacaggaggc gatatggggt ggatttcacg
     5281 cggctcactc cccgaaagct tcgaggaagc cgtattctca gcgcccttgc aatctgtact
     5341 cggtccggtg caaacgaaat acgggtatca tgttttcgaa gtacttgaaa ggcgtcgcgc
     5401 tcgggcgctc tcgttcgaag aggttcgcga tgccgtgcgc gccgaactgg ttgaacacgt
     5461 tatggctacc gaagcggctt ccctgaaggc gcgcttcccg gttcttcttg taccttccgc
     5521 tctcgaaaaa tcatttgcgc ggtgatacct tctctctaac acataacatg atgaaatacc
     5581 taaggtgcgg agcctgtccg cacctttttt tattctcttc tccgtccgaa actcatggac
     5641 agaagaaaat aatctcttat aataaaagga caagcgtctt cttgcaaagg cgttatcccg
     5701 ctctttcgga aaggatggat tgcgtatgga acaaacatct gcaaagacac ctacttttgg
     5761 tcaagctctt tttgtacttc ttgtggacgc tgctattatc agttatgggg ttctcgatat
     5821 tcattttgaa tcgggggcgg tgttcggtct cgccctctcc gcgcatattc cgctcttcct
     5881 cgccgcagtc tttaccgcca ttatgggagc tatttttgtc ggtaaaccgt ggcgtgaagt
     5941 tgaggaagga atgatcaacg gtattactgt cgcgcttcag gccgtcctta tattgcttgc
     6001 agtgggaggt cttgttgggg catggattct gagcggcgtt gtgcccacgc tcatttttta
     6061 cggtctcaac cttctgtctc ctcagtactt cctcctggca tctctcctga tctgcgccat
     6121 tgtctctctt tccacgggaa gttcctggag cacgagcgga accgtgggaa tagcgctcat
     6181 gggaataggc gcagggcttg gagtacccgc tcctgtaacg gcgggctttg taatctcggg
     6241 cgcgtatttc ggcgataaaa tttctcctct ctccgataca acgaaccttg ctcctgctgt
     6301 agccggttcg gaacttttta cccatatcag ggcgatgctc tggactaccg gtcccaccct
     6361 gatcatcgtt gccggaatcg cctattatat gggaagcggg tacactgggg gcgagatgga
     6421 cgcctccagg atctcgatta ttcaacagtt gatgtctgca gagttctcca tctcgatgta
     6481 cggtttcctt cctccgcttc ttgtcctcgt ccttgcggcg atgaagatcc ccgctatccc
     6541 cggtattgtc gccggcatgc ttctggcttc cgttatggcg cttttccagg gaatcagtat
     6601 tccggatgtt ttgagcgctc tgcattctgg atactctccc tccatgatag ccgaggtggc
     6661 cgaagccggg gatatctctt cagtgaacga gcttcttaaa ggagcggcgg tcttctccgc
     6721 ggatgcgttc gaaactgtca aggaggttgc gggcatgctc aacggtcttc ttgaacgcgg
     6781 cgggctgacc ggaatgcttg agacagttgc gcttatcctc gtcgctttgg tacttggcgg
     6841 aattatggag acgtgcggtt tcctcgaagt tcttctggct cggcttatgc agagcgta
//
LOCUS       DAOPKU010000100         6882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11715_length_6882_cov_5.599824, whole genome shotgun sequence.
ACCESSION   DAOPKU010000100 DAOPKU010000000
VERSION     DAOPKU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11715_length_6882_cov_5.599824"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK849_04480"
     CDS             complement(<1..264)
                     /locus_tag="PK849_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65414.1"
                     /translation="MSRKTFLWTVTALLLLGAGIVIAQMALDALALNETSVAAESQEL
                     QGVQGADETTMVVQKTKMQKSGASGKKASASMDEIHKKKLPQYR"
     gene            complement(413..1471)
                     /locus_tag="PK849_04485"
     CDS             complement(413..1471)
                     /locus_tag="PK849_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPE65415.1"
                     /translation="MKRRSMVFPGVFLFFCVLAAPVFADVLPRTTTATRDIMLVEEFE
                     EKEEQTAVASPVWTGMDGEEQAARLGVEEMHKTDFSVSLSSGKSAQGDVYEIGDKIEF
                     TFTSDKDCYVTLLDFTPSGKIYVLFPNKWVSNNFVKAGESVVVPAAGQQFAMKLGGPA
                     GVDIVKAIATNVETKILDPENQKLFGPFSVLEDPKVATRDIMLIEEEPEAAAAASPET
                     PLEWTAASLAVFTKGDSPEKGGFGVSIEGNWIAKIWADRDAFLTGESIFVKLQSNRPA
                     TLVSLVNTGASGRENILLPEGVEKSVLPGAIAILPGKQDKWKLIAATEPGVDVLTAVL
                     RDESGAEMKVSFSITVEE"
     gene            complement(1599..4451)
                     /locus_tag="PK849_04490"
     CDS             complement(1599..4451)
                     /locus_tag="PK849_04490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE65416.1"
                     /translation="MSKRIALVTADVHGEERWGEDRFLEGLRTALEAKGMTADILPVL
                     HDESSYEALSDTYARYAALDLSSFDGVVSTTIPGCAAVHPNKVCHLMRTARRFYDMFD
                     TEHPSPSPEHRQLRRHLQELDRAAFKDLDTVFVASGEIRGRLLKYLGVDSEVLRPSTT
                     LGGLHTASYEFLLLPGRLHKWKRVDLVVKAMAYVKSQVELLICGEGEEDAALKALAAG
                     DARIHFLGRVSDEKLTVFYSRALAVPYVPVGEDFGFVALEAALCEKPVITCVDSGEPA
                     SMVYDGRTGFVCAADPKEIGRCIDSLAQHTDIASAMGRIGRGSVGHVSWEHVATVLVR
                     SLGFAGTSAISSPVLAGGGIPSPRPLVLDAQPLLPAIDGGRARALGLYGNAGYDVRYL
                     GVLEEGGEKRNLRLTPTLEELDIPLSQEHLAERDALRKQPGGILALDVSFPLLGHLTP
                     EYAATAERMLSEANAVIFSRPWCYPLVSRALAEKMILTVYDAHNVEGVMRCALMDDGA
                     SGTELVGFVGELESSLCSRADLILAPSHRDRLSLARLYEIPHERIRVVPNGAFTERVT
                     PASEERKITLKEAEGLTDAEKEIPVAVFMGSASQANVEAAEFILREVAPAMPHVVFLI
                     FGSASEAVACTAPQNARLLGVVDEERKRDLLSMADVALNPLFSDCGGNAKMFEYMAAG
                     LPVLATPPAVRGIDLGGTEVVAFSSRERFVDDLRVLLEEPTELARSGAAGRCVAEERH
                     SWEPISRDLGMLIAWHASHRRRRRPFFSVVVPGYERPKLLSRMARLLSVQTYTDFEVL
                     FIDQSRTPWNESGAYRPLDLLYHRIDVKGAARARNIGAFLSRGKVLVFLDDDCEPSPN
                     WLAAARPWFDDEANAGLEGMIRSDLGWNDDRGRGAQNDTVNSRTTNFFVRAEIFNKTG
                     GFDDLRFAEGVEPGLRLDGEGEVPMCRNAWIFRAPR"
     gene            complement(4448..6310)
                     /locus_tag="PK849_04495"
     CDS             complement(4448..6310)
                     /locus_tag="PK849_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65417.1"
                     /translation="MGAKPRRSFAETLYSLPLLGYCAELLVQLVRLPVVLREVNIRQT
                     RKDTVVQSRLDGIGNRVAALREGASLCRREMEAALSGKADADTVASISEDLSKKVDAE
                     RFDSFTLNLEAELSRKVSAESLEDLGASWETALSELREELSGKAGKDSLRAMENELQT
                     KANAGSTAAIEEKVERIIERLGALDGSAVLDRLESMELLLEGKADLEEVAKLLSAKLD
                     TDALEIWGKRLEDASAQLLEALSQKADVERVDALSESLRSKADGETLESLSAAKADVE
                     ELLSLKARFESLGAHTSELENSLHSLCASKADLDAVRSLGERFEGVAAQLGALEGESV
                     LNRLSSAESAIERKADLEIVKSLLSLKMDVEVLKSLGKSLEEASGQTFAALEKKADLH
                     SVESVASTLDLKADAESVRSLNEGLSVVNERLGGFASKEDFSKVEELERLLSRKADAD
                     AFEERLSALDAKADLKTVEEKLAQKADAEAVESLSKEFEQRLAVKADAAAVDEALAKK
                     AGKDALDEVQKSVGSELSGLHARIDEGREQASEEERRLVRFREEVKALIASSLERGAR
                     GQIEEAFRATFPYVQKMKGRFPAFQGIDTQIKKTTQRSGGQGTEDAQSRKRVKR"
BASE COUNT         1474 a         2120 c         1836 g         1452 t
ORIGIN      
        1 cctgtactgc ggcagcttct tcttatgaat ctcgtccatg gaggccgacg ccttttttcc
       61 cgacgcgccg ctcttttgca tcttcgtctt ctgtacgacc atcgtggtct cgtcggcgcc
      121 ctgaactccc tgcaactcct gactttccgc cgccaccgag gtttcattca gcgccaacgc
      181 gtcaagggcc atctgggcga tcacgattcc tgcgcccagc agcaagagcg ccgttacggt
      241 ccaaagaaaa gtctttcgcg acacgatatg caacctccta aaagatattt tgtccgagcc
      301 ggcccttccg aagagaaggc gctccttttc ctcgctatga aatacggagg cgaatacaaa
      361 aacaggaagc gggagaagac gccttcgccc gcttcctgta tgatggactc ccctactctt
      421 cgaccgtgat gctgaaagag accttcatct ccgctccgga ctcgtcccgg agcactgcgg
      481 tcaacacatc gactcccggt tcggtggcgg cgatcagctt ccatttgtcc tgtttgccgg
      541 gaagaatcgc gatggccccc gggagcacgc ttttttcaac gccttcggga agaagaatat
      601 tctccctgcc gctggcgccg gtgttcacca gagatacgag cgtcgcgggc cggttgcttt
      661 gaagtttcac aaatatggat tcgccggtca gaaaggcgtc gcggtccgcc cagatcttcg
      721 cgatccagtt tccctcgatc gacacgccga aaccgccttt ttccggagaa tcgcccttgg
      781 taaagacagc gagcgaagcg gcagtccatt cgagaggcgt ttccggcgag gctgccgccg
      841 cggcttccgg ttcctcctcg ataagcatga tgtctctggt cgccaccttg ggatcttcca
      901 gcacagagaa cggaccgaac agtttctgat tctctggatc aagtattttc gtctccacgt
      961 tggtcgctat ggccttgacg atatccacgc cggcaggccc ccccaacttc attgcaaatt
     1021 gctgccccgc cgcggggacg acgacgctct ctcctgcctt cacgaagttg ttcgataccc
     1081 acttgttggg gaacagcaca taaatcttcc ccgaaggcgt aaagtccaga agcgtcacat
     1141 agcagtcctt gtcggaggtg aaggtgaatt cgatcttgtc gccgatctca tagacatcgc
     1201 cttgagctga tttcccggaa gagagcgaaa cggaaaaatc ggttttgtgc atctcttcca
     1261 cgccaaggcg ggcggcttgt tcttctccgt ccattccggt ccataccggt gatgcaacag
     1321 ccgtttgctc ctctttttcc tcgaactcct cgacgagcat aatgtcgcgt gtagcggtag
     1381 ttgtccgggg cagaacatcc gcaaaaaccg gcgccgcgag tacgcagaaa aacagaaata
     1441 ctccagggaa taccatactt cttcttttca actgcaacac ctcctgtaat cgttctccgg
     1501 aacagcggga atcaaaaaac agaaaaatga cactcagtcg aattatacca cacggcagaa
     1561 taaaacgccg gacaaaatcc ccctccacgg ggaaaaagtc agcgtggcgc ccgaaaaatc
     1621 cacgcgttcc ggcacatggg aacttcgcct tccccgtcga gccgcaatcc cggctcgaca
     1681 ccctcggcaa aacgaaggtc gtcaaaacct cccgtcttgt taaaaatctc ggctctgaca
     1741 aaaaaattcg tcgtgcgcga gttcacggta tcattttggg cgccccgtcc ccggtcgtcg
     1801 ttccacccga ggtccgaacg gatcatgcct tcaagccccg cgttggcttc gtcgtcgaac
     1861 catgggcgcg cggccgccag ccagttcggc gaaggttcgc agtcgtcgtc gagaaagaca
     1921 agcacctttc ctcgtgagag aaaggcgccg atgttgcggg cacgcgccgc ccccttaacg
     1981 tctattctgt gatagagcag atcaagagga cgatacgctc ctgattcgtt ccatggggta
     2041 cggctctggt cgatgaagag cacctcgaaa tcggtgtagg tctgaacaga gagcaggcga
     2101 gccatgcgcg aaagcaactt tggacgttca tacccgggaa cgactacgga aaaaaacggg
     2161 cgtctccgcc tgcgatgcga agcgtgccat gcaatcaaca ttcccaggtc gcgggagatc
     2221 ggctcccagg agtgccgttc ttcggcgacg caccgtccgg ctgcgccgct ccgcgcaagc
     2281 tcggtgggtt cttcgagcag gacgcgcaga tcgtcgacga agcgttcgcg ggaggagaac
     2341 gcaacgactt ccgtaccgcc gagatctatc ccgcggacgg cgggaggcgt tgcaagcacg
     2401 ggaagaccgg ctgccatata ctcaaacatt tttgcgttac ccccgcagtc ggaaaagagc
     2461 ggattgagcg caacatccgc catcgacagg aggtctcttt ttcgttcttc gtccacgacg
     2521 ccaagaagac gggcgttctg cggagccgtg catgcaacag cttcggaagc gctcccgaag
     2581 atcaggaaga cgacgtgcgg cattgcggga gcaacttcgc ggagtatgaa ctccgcagcc
     2641 tcgacattcg cctgagaggc gcttcccatg aagaccgcga ccggaatctc tttctccgcg
     2701 tctgtcagcc cctccgcctc tttcaacgtg atttttcgct cctcgctcgc cggcgtcacg
     2761 cgctccgtga acgcgccgtt cggaacgact cgtattcgct catgggggat ttcgtacagc
     2821 cgggccaaag aaagccggtc tcgatgagat ggagcgagaa tcaggtccgc cctcgaacag
     2881 aggctcgact caagctcgcc gacgaagccg accagctcgg tacccgaggc tccgtcgtcc
     2941 atgagggcgc agcgcatcac gccctccacg ttgtgcgcgt cgtagacggt caggatcatt
     3001 ttttccgcaa gcgctctgga tacaagagga tagcaccacg gacgggaaaa aatcaccgcg
     3061 tttgcttccg agagcatgcg ctcagcagtt gctgcgtact cgggtgtgag atgcccaaga
     3121 agtggaaagg agacgtcgag cgcgaggatg ccccccggct gtttccgcag cgcgtctctc
     3181 tcggcaagat gttcctgaga gagagggatg tccaattctt ccagtgtcgg cgtgaggcga
     3241 agatttcgct tctcaccgcc ctcctcaaga actccgaggt agcggacgtc atatccggcg
     3301 tttccataga gaccgagcgc tctggcgcgt ccgccgtcga tagccgggag taggggctgg
     3361 gcgtctagaa cgaggggtcg cggagaaggt attcctccgc cggccaagac gggcgaggaa
     3421 atcgcgctcg ttccggcaaa acccagcgaa cgcacgagca ccgtggcgac gtgttcccac
     3481 gagacatgtc ctaccgaacc gcgcccgata cgccccatgg cagaggctat gtccgtgtgc
     3541 tgtgccaagg aatcgataca ccgcccgatc tccttcggat cagccgcgca gacaaagccg
     3601 gttcgcccat cgtacaccat gctcgcaggc tctccggaat ccacgcatgt gatcacaggt
     3661 ttttcacaga gggctgcttc aagggcgaca aaaccgaaat cctctccgac cggcacatac
     3721 ggcaccgcaa gcgcgcgcga gtagaacacc gtcagttttt cgtcgctcac tctcccgagg
     3781 aagtgaatac gggcatctcc ggccgcaaga gcttttagag ccgcatcttc ttctccttcg
     3841 ccgcagataa gaagctcaac ctggcttttg acatacgcca ttgccttgac aaccagatca
     3901 acccgcttcc atttgtggag gcggcccgga agcaggagga attcgtacga cgcggtatgc
     3961 aaacctccga gggtagtcga tggacggagg acctccgaat cgacgccgag atacttcagc
     4021 agtcgtcccc gaatctcgcc gcttgcgacg aaaacagtgt ccagatcctt gaacgcggca
     4081 cgatccagtt cctgcagatg gcggcgcaac tgtctgtgct ccggggaggg ggaagggtgt
     4141 tccgtatcga acatgtcgta aaaacgtcgt gccgttcgca taaggtgaca gaccttgttc
     4201 gggtggacgg cggcgcatcc gggaatcgtg gtgctgacga cgccgtcaaa ggaggaaaga
     4261 tcaagcgcag cataccgcgc atacgtatcc gacagagcct cgtacgatga ttcatcgtga
     4321 agaacgggga gtatatccgc ggtcattccc tttgcttcaa gagcggtacg gagcccctcc
     4381 agaaaacggt cttctcccca ccgttcttca ccgtgaacat cggccgttac gagagcaata
     4441 cgcttgctca tcgcttcacc ctcttcctgg attgtgcatc ttccgtcccc tgcccgccgg
     4501 aacgttgtgt cgtcttcttt atttgcgtat caatcccttg aaacgcagga aagcgcccct
     4561 tcatcttttg tacatacggg aaggtagcgc ggaacgcttc ttcgatttgc ccgcgcgctc
     4621 cccgttcgag ggaggaagcg atgagcgcct tcacctcttc gcggaagcga acaagtctgc
     4681 gttcttcctc gctcgcttgt tctcgcccct catcgatacg ggcgtgcagg ccgctcagct
     4741 cgctcccgac agacttctgg acttcatcca gcgcatcttt cccggccttt ttcgcaagag
     4801 cctcgtcaac ggctgcggca tccgctttta cggcaagacg ttgttcaaat tccttcgaga
     4861 gtgattcaac agcttcagca tcggctttct gcgcgagctt ctcctccacc gtcttcaggt
     4921 cggctttcgc gtcaagcgca gagagtcttt cctcaaacgc atcagcgtcc gccttcctcg
     4981 aaagcagccg ctccaactcc tcaacctttg agaaatcctc tttggaagca aaaccgccga
     5041 gacgttcgtt cacaaccgaa agcccctcgt tcaaggagcg taccgactcc gcatccgctt
     5101 tcagatcaag agtggaggcc acagactcga cgctgtgaag atccgctttt ttctcaagtg
     5161 cggcgaacgt ctggccggac gcctcctcca ggctttttcc aagcgatttc agcacctcga
     5221 cgtccatctt gagcgagagc agcgatttca caatctccag atcagcctta cgttctattg
     5281 ccgactccgc cgagctcaat cggttcaaga cgctctctcc ttcgagcgcg ccaagctgag
     5341 cggctactcc ttcgaaacgc tcgccaagag aacgcacagc atccaaatcc gctttcgacg
     5401 cacaaagaga atgcaaggaa ttctcgagtt cggaggtgtg tgcgccaagg gattcgaaac
     5461 gagccttcaa agagagaagc tcttcaacat ctgcttttgc cgcagacaac gattcgagcg
     5521 tttctccgtc cgccttcgag cgcagcgact cggaaagcgc atccactctc tccacgtcag
     5581 ccttttgaga aagcgcttct aacaactggg cggaagcgtc ctccaggcgc tttccccaga
     5641 tttccagagc gtccgtatcg agctttgctg aaagcagctt cgcgacttcc tcaagatccg
     5701 ctttgccttc aagaagcaat tccatggatt ccagacgatc caaaaccgca cttccgtcga
     5761 gggcgcccag acgctcaata atgcgctcca ctttctcctc aatcgccgct gtactcccgg
     5821 cattcgcctt cgtctgcaac tcgttttcca ttgcgcgcag gctgtccttg ccggctttcc
     5881 cggaaagttc ctccctgagc tcgctcaaag ccgtctccca tgaagcgccc aaatcctcaa
     5941 gagactccgc ggaaaccttt cgagacaatt ccgcctcaag attcaaggta aagctgtcaa
     6001 accgctctgc gtcaactttc tttgacaaat cctccgagat agaagccaca gtgtcggcat
     6061 ccgctttgcc cgagagcgcc gcctccattt cccggcggca gagcgaagct ccctcccgga
     6121 gcgcggcgac cctgttccct ataccgtcaa gtcgcgactg cacaacagta tccttcctgg
     6181 tctgccggat attcacctcg cggagaacaa cgggaagcct gactagctgg acaaggagtt
     6241 cggcacagta accgagcaga ggtaacgagt acagtgtttc cgcaaaggaa cgccggggct
     6301 tggcgcccac aaaggatggc ttcccttcag atttccgtga gttccaagag ggagctgcgc
     6361 gtttcgcttg cacgggagct gttttcgggg cttgagagtg gacggaagcg gtctgcggaa
     6421 aaacccggcc cgccttcttt tccaggcggg ggcggcgcgt tgatggatat acagaatctt
     6481 cccgccgccg tatggcaagt tcatcacgga gacgttcaat cagagcctgc cctttttgcg
     6541 gttgagcgct ctgtccggac ggaggaagag aattgtccgc agcattcggg gaactcgcgg
     6601 gttcccgccg tatgacgacg ggcagttcgg aagtcgtatc cgtccgggta tcggggagag
     6661 gcgcagattg ggaatcgccg gagacattcg gtctccctgc gtccgaggag cttcggggta
     6721 gagacgaaaa aagcagagcg ctctcttcat caagaacagc aggagatggc tcgccgcccc
     6781 catcaaaaaa aatagtattc tttttttcgt tcactcccga aattctcctt tccacgcggc
     6841 ggcgccctcc gaaaagcggt aaccttctga atgaatggct tg
//
LOCUS       DAOPKU010000101         6861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11761_length_6861_cov_5.352336, whole genome shotgun sequence.
ACCESSION   DAOPKU010000101 DAOPKU010000000
VERSION     DAOPKU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11761_length_6861_cov_5.352336"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2775)
                     /locus_tag="PK849_04500"
     CDS             complement(<1..2775)
                     /locus_tag="PK849_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HPE65418.1"
                     /translation="MIPDILKKTKGGASGSSLPNDELLVHQAESLILSGDLAAATEKI
                     GGAVSGESYAVRALLALLDDRRKDALALFEEGLKRLRKERGVRRIVFDSFSALYYPPL
                     LIASGDVKKAATYAELARQRSPYEPTYELLLCLAGFHSGKGSDKFFSVFSPARCKKEK
                     YKKGFLFHLALCLSWSSPEALIDPEYRSLFLNALDELRKSGNTFLLYCVELAEMLSLP
                     PEKQIPGRKLPLRDLIPRKEEWMVALGALTTLGKGTGGAKKKRERRILWEVDWDTEEN
                     GTIVSPEFIPVEQVMQSSGKWSKGRQVALKRLQRELVKEPWMTRQDLAAAGAVEEVGG
                     YDGWYYKRWFQFDGVKIFSALAGHPYLVRSGDGRPMEIVQEEPRLDLARTVGGYRVRL
                     SPHPAEGKYANVFLEEETPCRIRVVPFDEKHRRIAEILGKEGLFVPERGKEHILETLE
                     SLAETVPITSEIEGVEGSAVAVEPDSRIVVQLAPGEDGFFVSLAVRPLGTGTHLCRPG
                     EGGKTMMGRSDGQRIQTLRDLDAERRNRDEALVLCPALSEGEQTDENRWELDNPELCL
                     EFLLQLAGMGERVVVEWPKGGAMSVRSAVSPNAVKLSVATGRDWFALSGEIRVDEQTV
                     LGIKEACEMLAESKGRFLPLGNGEFLALTAELRKRLEEIGSFGDWRGKELRFSPLSAP
                     LFRKLEKETEGFGGDGEWAKQLALVEEAESIAPALPSTFKGVLRDYQTEGFQWLCRLA
                     HWGAGACLADDMGLGKTIQVLAVLLSRAQTGPSLVIAPVSVCPNWFQEAARFAPTLNL
                     KEFRYGDRQKILDDLEPFDVVVTSYGLLQQDGEMLHEVEWNTVVLDEAQAIKNAATKR
                     SAAAMRLKGKFRVITTGTPIENHLGELWNLFRFLNPGLLGSLDSFNRRFATPIVKYDD
                     KGARARLKKIL"
     gene            complement(2791..3795)
                     /gene="pdxA"
                     /locus_tag="PK849_04505"
     CDS             complement(2791..3795)
                     /gene="pdxA"
                     /locus_tag="PK849_04505"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPE65419.1"
                     /translation="MKRTVIAVSMGDPAGVGPEIALASMADAEVWERGTPLLVGDLSV
                     LEAVKEALGMKMRLRPVEDPADAAGSPESLPVIDLKMVPDLTALSVGKVSALGGKASV
                     AYIQKCVDLCMTGQAAGIATTPINKESLKAAKVHYIGHTEMLAEMSGAPKSYTMFIVD
                     KLRILFHSRHLSLRRAIETLNTDDVVHSIETAAKCLDSIGLPSGTIALAGLNPHSSDG
                     GLFGDEEARILAPAVEEAKRRGINVVGPVPADSVFYFGLQGKYDVVVSLYHDQGHIAS
                     KTYDFYRTVSVTFGLPFIRTSVDHGTAFDIAWKGIANPVSMKEAMLACFDIAPRYRPF
                     "
     gene            complement(3831..5051)
                     /locus_tag="PK849_04510"
     CDS             complement(3831..5051)
                     /locus_tag="PK849_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HPE65420.1"
                     /translation="MNGFAVLCDDLTGSSVQSILLKDRGVPVRQIIRLSGSPPSPPGE
                     GEALVINCDTRRRSREEAEKIFMEILGSFPPDIGIAKRIDTTLRGHLGSETEAILKTR
                     PGAVAMVVPAYPSSGRTTVGGYHLLNGSLLERTEVARDPLWPISTSYVPSYFKGLLVA
                     RMHMECVKRSEEEVIVELRKLLSEGARVVVADAMTDSDIEKLASASFASKEDIIPVDP
                     GPFTAALLSRHLSRRSPGTVLAVIGSTSEKTRKQIAWLDGKIPSEEFVFSPSETTEKA
                     ASRLREFLTSLDRTVSFLLLRPDPRVEKGSEERTARTLAFLGKEALTVLGGHVRGILL
                     SGGDTAAMFFEAAGATALLPETEIQPLMMGGKILDGAFAGLKTVTKGGLVGEEDAIYK
                     AALWLRLAPEATRP"
     gene            complement(5056..6342)
                     /locus_tag="PK849_04515"
     CDS             complement(5056..6342)
                     /locus_tag="PK849_04515"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE65421.1"
                     /translation="MATIMFSVLIVLIFLNIPVAVSIGLASIAGITFGNLPLNPAVVA
                     QRMFTSADSFPFMAIPFFMLAGGLMEHGGISRRLVKLASALVGGYRGGLGLITILASA
                     FFGAISGSNPATVAAIGGIMVPSMIKKGYPPAFAASIAAAGGTLGVVIPPSIPMITFG
                     VVAGVSIGDLFLAGFGPGVLLALVLSLVVVMQSIKLDIPKEPAREKGELLAAVKDSIL
                     AIIMPVIILGGIYGGVFTPTEAGAVAVIYSLLVSAFIYEELDLRTIRTVIIKAGISTS
                     VVFFVIATSQSMSWLITVSRVSAELAAWIVSVSSNPFVLVTMINFILLFLGVFLETQA
                     IILLMAPLLLPITASIGMNPLMLGIIMVVNTSVGMITPPMAVNLFVAVSLVKDHNVRI
                     EQITRKILGFLAAELIAIMLISNFPVLSLGLVQLLK"
     gene            complement(6343..>6861)
                     /locus_tag="PK849_04520"
     CDS             complement(6343..>6861)
                     /locus_tag="PK849_04520"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE65422.1"
                     /translation="AIFPTRGSFMRKVFDNFEEYVLLLLFPLMVIVVFIATLARYFNL
                     FPMFWGEEVARYIMVFMAYIGAGLGMKKGAHVGVSFFTDKIRGRKLRILFETMRTGII
                     LFFCLMIMYYYKGIIVHQIFMGQTTPALYIPMWIPYAAVPLGMFLVALRAFQAYRETV
                     REIRSAAPSEVK"
BASE COUNT         1380 a         2085 c         2068 g         1328 t
ORIGIN      
        1 gaggatcttc ttcagccggg cgcgcgctcc cttgtcgtcg tacttcacga tcggtgtggc
       61 gaagcggcgg ttgaagctgt cgagcgagcc gagcagcccc ggattgagaa agcggaagag
      121 gttccacagc tcgccgagat ggttttcgat cggggtgccg gtggtgatga cccgaaactt
      181 ccccttcaag cgcattgccg ccgccgaacg cttcgtcgcc gcgtttttga tggcctgcgc
      241 ctcgtcgagg accactgtgt tccactcgac ttcatggagc atctctccgt cctgttgaag
      301 cagcccgtag cttgtcacca ccacgtcgaa cggctccagg tcgtcgagga tcttctgccg
      361 gtcgccgtag cggaattcct tcaggttcag agtgggcgca aaacgcgcag cctcctgaaa
      421 ccagttcggg cagacggaga cgggagcgat cacgagcgaa ggaccggtct gcgcgcgaga
      481 gaggaggacg gcgagcacct ggattgtctt gccaagcccc atatcgtccg cgagacatgc
      541 gcctgcgccc cagtgcgcga ggcggcagag ccactggaat ccttcggtct gatagtcgcg
      601 caggacgcct ttgaacgtcg agggaagcgc gggagcgatc gactcagctt cctcgacgag
      661 cgcgagctgt ttggcccact cgccgtcgcc gccgaaaccc tccgtttctt tctccagctt
      721 ccggaaaagc ggcgccgaga ggggggagaa gcgcagctcc tttccccgcc agtcgccgaa
      781 cgagccgatc tcttcaagcc gcttccgcag ctccgcagtg agcgcgagaa actcgccgtt
      841 cccgaggggg aggaagcgtc ctttgctttc ggcgagcatc tcgcacgcct ctttgatgcc
      901 cagaacggtc tgttcgtcca cgcgaatctc cccggagagc gcgaaccagt cgcgccccgt
      961 agccacggag agctttaccg cgttcgggga gacggcggag cggacgctca tcgcgcctcc
     1021 cttgggccac tcgacgacga cgcgctctcc cattcctgca agctggagca aaaattcgag
     1081 gcagagttcg gggttgtcaa gctcccatct gttctcgtcc gtttgttccc cttcgctcag
     1141 agcagggcag agcaccaacg cctcgtcgcg attgcgccgc tcggcgtcga gatcccgaag
     1201 cgtctggatg cgttgcccgt cgctccggcc catcatcgtc ttgccgccct cgcccggcct
     1261 gcagaggtgc gtgcccgtcc cgagagggcg gacggcgagc gagacgaaaa aaccatcttc
     1321 gccgggggcg agctggacga cgatgcgcga gtccggctcc accgcgacgg cgctcccctc
     1381 cacgccttcg atctcggagg ttatgggaac agtctccgca agcgactcca aagtctccag
     1441 aatatgctcc ttcccccgct ccgggacgaa gagaccttcc ttgccgagga tctccgcgat
     1501 gcggcggtgc ttctcgtcga agggaaccac acgtatccgg cagggagtct cctcttccag
     1561 gaagacgttg gcgtacttgc cctcggcagg gtgcggagag agacggacgc ggtagccccc
     1621 gaccgtcctc gccaggtcga gtctcggctc ctcctgcacg atctccatcg gcctgccgtc
     1681 cccgctccgg acaagatacg ggtgccccgc gagcgccgag aagattttca ccccgtcgaa
     1741 ctggaaccat cgcttgtagt accagccgtc gtagccgccg acctcctcga ccgctcccgc
     1801 cgctgcgaga tcctggcgtg tcatccacgg ttccttgacg agctccctct gaagacgctt
     1861 caatgcgacc tgccgtccct tgctccactt tccggaggat tgcatcacct gttcaacggg
     1921 gatgaactcc ggggagacga tcgtgccatt ctcttcggta tcccagtcca cttcccagag
     1981 aatgcgccgc tcccgctttt ttttcgctcc gccggttccc ttcccgagcg tcgtcaacgc
     2041 cccgagcgcc accatccact cctccttgcg ggggatgagg tcgcgcaacg ggagtttcct
     2101 tccggggatt tgcttctccg gcggaagcga gagcatctcg gcaagttcga cgcaatagag
     2161 caggaacgta ttgccggact tgcgcagttc gtcgagggcg ttcaagaaga gggaacggta
     2221 ctccggatcg atcagcgctt ccggggaaga ccatgagaga cagagcgcga gatggaagag
     2281 aaatcccttt ttatattttt cctttttgca acgggcggga gagaatacgg agaagaattt
     2341 gtcgctcccc ttgccggagt ggaagcctgc aaggcagagc agcagttcgt aggttggttc
     2401 gtacggggaa cgctgtctgg caagttcagc gtacgtcgcc gctttcttga cgtctccgga
     2461 ggcgatcagc agcggcgggt agtaaagggc ggaaaaagag tcgaagacga ttcgtctgac
     2521 gccgcgttcc ttccgcagac gcttcaggcc ttcttcaaaa agcgccaggg cgtccttgcg
     2581 ccggtcgtcg agaagagcga gcagcgcccg gacagcgtac gattcgcccg acacggcgcc
     2641 cccgatcttt tccgttgccg ccgccaagtc tccggagagg atgagcgatt ccgcttgatg
     2701 tacgagaagc tcgtcattgg gaagggagga gccggaggcc cctcccttcg tcttcttcag
     2761 aatatccggt atcacgtcgc gcgctcccgg ctagaacgga cggtagcggg gggcgatgtc
     2821 gaagcaggcc agcatggcct ccttcatgct caccgggttg gcgatcccct tccatgcgat
     2881 gtcgaaggcg gttccatggt cgaccgacgt gcggatgaac ggcagtccga aggtgacgct
     2941 caccgtccgg tagaagtcgt aggtcttcga ggcgatatgc ccctggtcat gatagagcga
     3001 gaccacgacg tcgtactttc cctgaaggcc gaagtagaag acgctgtccg ccggaaccgg
     3061 cccgactacg ttaatccctc tccttttggc ctcctcgact gccggagcaa ggatgcgcgc
     3121 ctcctcgtcg ccgaagagcc ccccgtccga cgagtgcggg ttgagcccgg cgagagcgat
     3181 cgtacccgac ggaaggccga tggagtcgag gcacttcgcc gccgtttcga tggagtggac
     3241 cacatcgtcc gtgttcagcg tctcaatggc gcgccgcaac gagaggtgac ggctgtggaa
     3301 gaggatgcgg agcttgtcga cgatgaacat cgtgtagctc ttcggagcgc ccgacatctc
     3361 ggcgagcatc tccgtgtgcc cgatgtagtg gaccttggcg gctttcagcg actccttgtt
     3421 gatcggggtg gttgcgatgc ccgcagcctg ccctgtcatg cagaggtcga cgcacttctg
     3481 aatgtatgct acggaggctt tgcctccaag tgcggaaacc tttcctacgg agagagccgt
     3541 aagatctgga accatcttca ggtcgatcac cggaaggctc tccggcgatc ccgccgcgtc
     3601 cgcagggtcc tccacgggcc ggagtcgcat tttcataccc agcgcttcct tcacggcttc
     3661 aaggaccgag aggtcgccga cgagcagcgg cgttccccgc tcccaaactt cagcatcagc
     3721 catgctcgca agggcgatct ccgggccgac tcctgcgggg tcgcccatgc tgactgcgat
     3781 cactgtacgt ttcatgaaat attactccct ttccttcccc ctccgctctt tcagggacga
     3841 gttgcttcgg gggcgagtct caaccagaga gcggccttgt aaattgcgtc ttcttcgccg
     3901 acgaggccgc ccttggtgac tgttttcaaa ccggcgaacg caccgtcgag aattttgccg
     3961 cccatcatca gcggctgtat ctcggtttcg ggaaggagcg cggtcgctcc ggccgcctcg
     4021 aagaacattg ccgccgtgtc tcctccggag agaagtatac cccgaacatg acctccgagt
     4081 acagtaaggg cttctttccc gaggaaggcc agggtacggg cggtccgctc ctccgagcct
     4141 ttttcgacgc gcggatccgg tcgaagcagg aggaatgaga ccgtcctgtc caaactcgtc
     4201 agaaactcgc ggagacgaga tgcagccttc tccgtcgtct ccgaaggcga gaagacgaac
     4261 tcttccgacg gaatcttgcc gtcgagccag gcgatctgtt ttctggtctt ctccgacgta
     4321 cttccgatga ctgcgagcac agtacccgga cttctccggg agagatggcg cgaaaggaga
     4381 gccgccgtga aagggcccgg gtccacaggt atgatatcct ccttggaggc aaacgaggct
     4441 gatgccagct tttcaatatc gctgtccgtc atggcgtccg ccacgacgac gcgcgcccct
     4501 tcagagagaa gtttcctcag ctcgacgatg acttcttcct cgctcctctt gacgcactcc
     4561 atgtgcatcc gggcgacgag gagccccttg aagtaggatg gaacgtagct ggtagagatc
     4621 ggccagaggg gatcgcgggc gacttctgtc cgttccagca gggagccgtt caaaagatgg
     4681 taccctccga ccgtcgtcct cccggaggag gggtaggcgg ggacgaccat ggcgactgct
     4741 ccggggcggg ttttcaggat cgcctccgtt tcgctcccga ggtgtcctct gagcgtggtg
     4801 tcgatacgtt ttgcgattcc aatatccggc ggaaaagacc ccagaatctc catgaagatc
     4861 ttctcggcct cttcccgtga tcgtcgccgc gtatcacagt tgatcaccag cgcctccccc
     4921 tccccgggag gagaaggcgg actgccggag agacgtatta tctgacggac gggaactccg
     4981 cggtccttca acaaaatgga ctggacggaa gaaccggtga ggtcgtcgca gagcacagca
     5041 aagccgttca tagcgctatt tcaacagctg aacaagcccc agggaaagaa cggggaaatt
     5101 actgatgagc atgatcgcta tcagttccgc cgcgaggaag ccgagaattt tccgcgtgat
     5161 ctgctctatg cgcacgttat gatccttcac cagcgagacc gcgacgaaca ggttcaccgc
     5221 cattggcgga gtgatcatcc ctaccgaggt gttcaccacc atgatgatcc cgagcatcag
     5281 cggattcatg ccgatggacg cggtgatggg aaggagcagc ggcgccatca gcaggatgat
     5341 cgcctgcgtc tccaggaaga cgccgaggaa gaggaggata aagttgatca tcgtaaccag
     5401 gacaaacggg ttcgacgaca cggaaacgat ccatgcggcc aactccgccg ataccctgct
     5461 caccgtgatc agccacgaca tcgactgcga cgtggcgatg acgaagaaga cgacggaagt
     5521 actgatgccc gctttgatga tgaccgtccg gattgtccgc agatcaagtt cttcgtatat
     5581 gaacgcgctg actaaaagac tgtagatcac cgcgaccgcg cccgcctcgg tcggggtgaa
     5641 gaccccgccg tagatgccgc cgaggatgat gacaggcatg atgatggcga gaatggagtc
     5701 cttgactgca gcgaggagtt ctcccttttc tcttgcaggt tctttcggga tgtcgagctt
     5761 gatggactgc atcaccacga caaggctgag gacaagcgcg agaaggacgc cgggaccgaa
     5821 tcctgcgagg aaaagatcgc cgatggagac ccctgccaca acgccgaagg taatcatcgg
     5881 aatggaggga ggaatgacca cgccgagcgt tcctcctgcg gccgctattg aggctgcgaa
     5941 tgccggagga taccctttct tgatcatcga ggggaccatg atgccgccga tggccgccac
     6001 tgtggccggg ttcgacccgc tgattgcgcc gaagaacgcg ctcgcgagga ttgtgatcag
     6061 gccgagccct cccctgtagc cgccgacgag cgcggatgcg agtttcacaa gtctgcgcga
     6121 gatgccgccg tgttccatga gtcctcctgc aagcatgaag aaggggatcg ccatgaaagg
     6181 gaaggagtcg gcggaggtga acattctttg tgccacgacg gcaggattca gcggcaggtt
     6241 gccgaaggtg atccccgcga tggatgccag tccgatgctc acggccaccg ggatgttcaa
     6301 aaagatgagc acgatgagga cggagaacat gatagtggcc atttatttca cctccgaagg
     6361 ggcggcggaa cggatctccc gcacagtttc acggtatgcc tggaatgccc gcagcgcgac
     6421 gaggaacatc ccgagcggga cggctgcata gggaatccac atggggatgt acagcgccgg
     6481 agttgtctgt cccatgaaaa tctggtggac aatgattccc ttgtagtagt acatgatcat
     6541 caggcagaag aagaggataa taccggtccg catcgtctcg aagaggatgc gaagtttccg
     6601 gccccgaatt ttgtcggtga agaagctcac tccgacgtgc gcccccttct tcatccccag
     6661 tccggcgccg atgtaggcca tgaagaccat gatataccgc gctacctcct caccccaaaa
     6721 catcggaaag aggttgaaat aacgcgccag cgtggcgatg aaaaccacga tcaccatcag
     6781 agggaaaaga agcaacagca cgtactcctc aaaattatcg aagacttttc tcatgaatga
     6841 cccccgtgtc ggaaaaatgg c
//
LOCUS       DAOPKU010000102         6841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11811_length_6841_cov_4.606248, whole genome shotgun sequence.
ACCESSION   DAOPKU010000102 DAOPKU010000000
VERSION     DAOPKU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11811_length_6841_cov_4.606248"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..168)
                     /locus_tag="PK849_04525"
     tRNA            complement(94..168)
                     /locus_tag="PK849_04525"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(134..136),aa:Ala,seq:cgc)
     gene            402..2090
                     /locus_tag="PK849_04530"
     CDS             402..2090
                     /locus_tag="PK849_04530"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HPE65423.1"
                     /translation="MSFGPELITVLAREVNAREMPARIHKIETGDVWAALKIGGEKWL
                     FFSWHAVHYGLGIVGEETVAALKKMKTPKSSFGEALKRNFLNGTVLSAKQLYNDRILL
                     LETERLVGAGFTVSNFLIFEGMDRNSNLILLDEKQCVLDAAKHIHPDVNRYRTVLPGN
                     HYTPPPPRDGAVWSTKREHLSLLDIRTLIGIGRPLVKTIIEHWESFPLSFWSEAIDGL
                     LGGAPSALPLGLQRIGNYITVFPVLFKDAEALDGSPIERCGKETAGKLLSLHREKGLA
                     SVRKAIEKEIRSRERHADGLRKQLSLSGHAEEFKKYGELLLGSAHCIRQGADTFTVTD
                     WETQREITIVLDEKLSPTANAERYFKKYKKGKIDVAAVRKKIEAIEDGIRELEEQMGA
                     LESIDDPRLLAATAQDILEWLAPPKKTGAKKKKEELPPHIRFSYAGNEIFVGLNARGN
                     RYVTFKAASPGDLWFHIHEIPGAHIILKTPGEVYDDAKDSILLAASLAAWFSKARLST
                     KVQVDFTRRKNVRSIPGSAIAHVTYTNPETVQISPGLWKEYPEAVRSIHLATDR"
     gene            complement(2023..3048)
                     /locus_tag="PK849_04535"
     CDS             complement(2023..3048)
                     /locus_tag="PK849_04535"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPE65424.1"
                     /translation="MPKGMREEGRFPKESVPESSSPEADEEIAVTEAGGRSETLIVQE
                     VEAGSRLDLFLALRLGITRSFARKLLDSGNIDLSSAKKVKPGLKITPGLVVSATLPAP
                     ETMDLEPENVPFTVVHEDPWLIVVNKPAGVVVHPAPGNWRGTLVHGLLFRFDSWGVFN
                     NVHRPGIVHRLDGPTSGLLVVAKDQETLDGLQKQFRLREVEKRYLALVEGKIRHTSGR
                     IELPIGRSSSNRLRMTVDPDGKKAVTEFRVLWSRSGYTFVECHILTGRTHQIRVHMSH
                     IGHPLVGDALYGASGKDAEKLGRVFLHSWKLSFRHPVSRKTVWFTCPLPGELTGQLQD
                     ILSTNRD"
     gene            complement(3029..5821)
                     /gene="ileS"
                     /locus_tag="PK849_04540"
     CDS             complement(3029..5821)
                     /gene="ileS"
                     /locus_tag="PK849_04540"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPE65425.1"
                     /translation="MSTDYKSTLSLPETAFPMRANLAKREPGFLDFWKERDIYGKMLK
                     KREGAPSFVLHDGPPYANGHIHIGTAFNKILKDFIPKYKAMKGFFSPYVPGWDTHGLP
                     IELHVIKSLGVSKDTIDPVLLREKCTEYALQFRDIQRDEFVRLGVLGDWENPYMTLRP
                     EYEAAQLGAFADLVGKGLVYKGKKPVYWCIDCQTALAAAEIEYWDETSPSIFVAYSLS
                     GAEALLPSLAGEDVNIIVWTTTPWTLPASMAVAIGPDHEYVFVRAGKKVYVFAKALAA
                     SVAKETGIVFEDELATVKGAALQQLHAVHPFYPDRTLPLLPADYVLLDTGTGCVHTAP
                     GHGVEDYETGVKFGIEVYNPVDDTGHFIQGAGLVDGLSLEEGSKKIFEILTANGRLLG
                     NSKIQHSYPHCWRCKKPVIFRSTDQWFVAVADFRNEALKTIENDVRWIPDWGKDRIFN
                     MVRDRSDWCISRQRIWGVPIPAFYCNDCGKPILTEDRIHAVQAFVKEQGCSAWWRMTP
                     GELLGNLCVCPHCKSTNLRKETDIMDVWFDSGTSHAAVLKIRPELKWPADMYLEGSDQ
                     HRGWFQTSLLTSVGMHGRAPYEQVLTHGFIMDGEGRKMSKSLGNVVAPQEVIKEYGAD
                     ILRLWVASTDYRNDVRISNTIIKNLSESYRRIRNTARYLLANLNDFAPADAVPFNEMV
                     EMDRWILMKLNSIIEKANSAYDNYEFHIPTFAIHQFCVNELSSFYLDASKDRMYADGK
                     NSLSRKSGQTAMWYVLSALTRMLAPVLSFTAEEIWQEMLKIDVSLPESVFLSDWPDVQ
                     EGIRNQDIEEKWDKALAVRGAVSRVLELARSGGIIGHALDARVEVEKQNTASVAATFS
                     EEDFKTFSIVSGFRWVDQVSQMSIVQTDEETGIRVGVDKALGEKCPRCWQYTESPDAE
                     GLCPRCSRVLRAQGDA"
     gene            complement(5917..>6841)
                     /locus_tag="PK849_04545"
     CDS             complement(5917..>6841)
                     /locus_tag="PK849_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HPE65426.1"
                     /translation="TRLGLRGLPRDQLPPLLRLLRQKGHLLIPLVGIVYFLIGGYTPL
                     KAAYNGIIITFVVSWLNKETRLTLPRLIAALESGARSALGVACACATVGIIVGTATLT
                     GLGLRIASAIVTLAGGNLLLTLLLTMVACILLGAGLPTTANFIVTSTMAAPALFQLGV
                     PPMAAYMFVLYFGIAADLSPPVALAAYAGAGIAGAEPMKTGVTAVKLALAGFIVPFIY
                     VFNPILVLVDYSFVPFALAVGTAMLGVVLLGMSTVGYFRGVLSLWKRALALAGALFLL
                     IPGWQSDLFGIAVLVLLYILQSFSSRKAALG"
BASE COUNT         1666 a         1897 c         1791 g         1487 t
ORIGIN      
        1 ttcgaccccc tgctcccaaa gcaggtgcgc taaccagact gcgctacgcc ccgattcatt
       61 gtaaagaagc aatcgaacta accgagcgaa atctggtgga gctgggggga ttcgaacccc
      121 cggcctcttc cgtgcgaggg aagcgcgctc ccactgcgct acagccccgg tcagaccaac
      181 ggcgattatt ttatacggcg atcccgttcc tgtcaagcct cgactttcgt ggagaatttc
      241 gactttctgt ttaccgacag catttttgaa tgccggaacc tttcctgcac cggccttctg
      301 ggtatattcc actgccagag gcgaaatttc tcctggagat ccggggaagt ggtatacttt
      361 tacccctgaa agaattttga tctgaaaacg gagtgatgct cgtgtctttc ggtccggaac
      421 tcattacggt tcttgcgcgt gaagtcaatg cacgcgaaat gccggcgcga atacataaga
      481 ttgaaacggg cgatgtgtgg gccgcactga agataggcgg tgaaaaatgg ctcttctttt
      541 cgtggcatgc tgttcattac ggactgggta tcgtcgggga agaaactgtt gctgcactga
      601 agaaaatgaa gacgcccaag tcgtcattcg gcgaagccct gaaaagaaac tttctcaatg
      661 gaacagttct ttcggcaaaa cagctataca acgaccgcat tctgctgctg gaaaccgaac
      721 gccttgtcgg cgccgggttc actgtctcga attttttgat tttcgaaggg atggatcgta
      781 acagcaacct gattcttctc gacgaaaaac agtgcgtcct ggatgcagcg aaacacattc
      841 atccggatgt gaaccggtac cgtacagtat tgccggggaa tcactacacg ccccctcccc
      901 cgcgggacgg ggctgtgtgg agtacgaaaa gagagcacct ttcccttctc gatatacgta
      961 ccctcatcgg tatcggcaga cctcttgtta aaaccataat tgagcattgg gagagcttcc
     1021 ctctctcctt ttggtctgag gcaatcgacg gattgcttgg cggcgcgccg agcgcacttc
     1081 ccctcggtct ccagaggatc ggtaattata taacagtctt cccggtgctt ttcaaagacg
     1141 cagaagcact ggatggaagt ccgattgaac gctgcgggaa agaaaccgct gggaagctcc
     1201 tctcgctgca ccgggaaaaa ggcctcgcat ccgtacgcaa agcaatagaa aaagagatac
     1261 gaagccgtga acgccatgct gacggattac gcaaacagct ctcactttcc gggcacgccg
     1321 aagagtttaa aaagtacgga gagcttcttc tcggctccgc gcattgtatc cggcagggag
     1381 cagacacgtt taccgttaca gactgggaga cgcaacggga aatcacgatc gttctggacg
     1441 aaaaactctc gccgacagcg aatgcggagc ggtatttcaa aaaatacaag aaaggaaaga
     1501 tagatgtagc cgctgttcgt aaaaaaatag aggcgatcga ggacggcatt cgcgaactcg
     1561 aagaacagat gggcgctctt gagtcgattg atgatccgcg gcttctggca gccacggcgc
     1621 aggatatcct ggagtggctc gcgcccccga aaaagaccgg ggcgaagaaa aagaaggagg
     1681 agctcccccc ccatatccgg ttctcttatg caggcaacga gatctttgtc ggactcaatg
     1741 cgcgtgggaa cagatacgtc acgttcaagg ccgcatctcc gggagatctg tggtttcata
     1801 ttcacgagat cccaggagcc catatcattt tgaaaacgcc gggagaggtc tatgatgatg
     1861 caaaggactc aattcttctg gccgcgtcgt tggcagcgtg gttcagcaag gccaggctct
     1921 caacaaaagt acaggttgac ttcaccaggc gcaagaacgt acgttcaata ccagggagcg
     1981 caatagcaca cgtgacatac acaaatcctg agacagtgca gatcagtccc ggtttgtgga
     2041 aagaatatcc tgaagctgtc cggtcaattc acctggcaac ggacaggtaa accaaaccgt
     2101 ttttcgggaa acggggtgcc ggaaggagag tttccatgag tgcagaaaaa ctctcccgag
     2161 cttttccgca tcttttcccg aagctccgta cagggcgtct ccgaccagag gatgccctat
     2221 atggctcatg tgaacccgta tctgatgcgt tcggcccgtc agaatatgac actcgacaaa
     2281 agtgtacccg ctgcggctcc acagaacccg gaactccgtt acggcttttt tcccgtcggg
     2341 atcgacggtc atacgcagcc tgttgcttga agaacggccg atagggagct cgattctgcc
     2401 gcttgtgtgc cggattttcc cttcgaccaa agccaggtat cgtttctcta cttctcgcag
     2461 acgaaactgc ttttgcaacc cgtcgagcgt ttcctggtcc tttgcaacga cgagcagccc
     2521 ggaagtcggt ccgtccaggc gatgcacgat tccgggacga tggacgttgt tgaagacgcc
     2581 ccatgaatca aagcggaaaa gcagtccatg cacaagcgtg ccgcgccagt taccgggggc
     2641 cgggtgcacg acgacgcccg cgggtttatt gacaacgatc agccatggat cctcgtggac
     2701 taccgtgaaa gggacgttct ccggttccag gtccattgtc tcaggagcgg gcagcgtcgc
     2761 actgactacg aggccgggcg ttatcttcaa acccggtttc actttcttcg ctgaagacag
     2821 gtcgatattc cccgaatcga gcagtttccg ggcaaaggaa cgcgtaatac ccagccgaag
     2881 cgccaagaag aggtcgagac ggctccccgc ctcgacctct tgtacaataa gcgtctcgct
     2941 cctgcccccg gcttcagtaa cggctatctc ctcatccgcc tcgggagagc tcgattcggg
     3001 aaccgactct ttcgggaaac gcccttcttc acgcatcccc ttgggcacgg agcacacgcg
     3061 agcatctggg gcagaggcct tccgcatcgg gactctcggt atactgccag catcgcgggc
     3121 acttttcccc gagcgcttta tccacaccaa ccctgattcc tgtttcttcg tcggtctgca
     3181 cgatgctcat ctgcgaaacc tgatcaaccc accggaaacc ggagacgatg ctgaaggtct
     3241 tgaaatcttc ttcagaaaat gttgcggcaa ctgaagcggt gttctgcttt tcaacctcaa
     3301 cccgcgcatc aagagcatgc ccgataatac cgccgctcct tgcgagctcg agaaccctgc
     3361 tgaccgcacc acggaccgcc aacgctttat cccatttctc ttcgatatcc tggttacgga
     3421 tcccttcttg cacatccggc cagtcggaga ggaagacgct ctcaggaagc gaaacgtcaa
     3481 tttttagcat ctcctgccag atctcctcag cagtgaaact gagcaccggc gcaagcatcc
     3541 gtgtaagggc ggaaagcaca taccacatcg cggtctggcc gctcttgcgg gaaagggaat
     3601 ttttgccgtc cgcgtacatc ctatccttac tggcatccag atagaaagag ctcagttcgt
     3661 tcacgcagaa ctggtggatt gcgaacgtgg gaatgtggaa ttcatagttg tcatatgcgc
     3721 tgttcgcctt ctcgatgatc gagttgagct tcatgagaat ccaacggtcc atctcaacca
     3781 tttcgttgaa cgggacggcg tcggccggag cgaagtcatt caggttcgcc agcagatacc
     3841 tggccgtatt gcggattcga cggtacgatt cactcaaatt ctttataatg gtgttggaaa
     3901 tacggacgtc gtttctgtaa tccgtcgagg caacccaaag gcggagaatg tcggcgccgt
     3961 actccttgat gacctcttgc ggggcaacaa cgtttccgag ggacttcgac attttgcgtc
     4021 cttcgccgtc cattatgaaa ccgtgcgtga gcacctgctc gtacggcgcc ctgccgtgca
     4081 tcccgaccga agtcagcaac gaagtctgga accatccacg gtgctggtcg cttccttcaa
     4141 ggtacatatc ggcaggccat ttcaattcgg ggcggatttt cagcacggcg gcatgactcg
     4201 ttccggagtc gaaccagaca tccatgatgt ccgtctcctt acggagattc gtacttttgc
     4261 agtgggggca gacgcataaa ttgccgagca gctccccggg agtcatcctc caccatgccg
     4321 aacacccttg ttctttaacg aacgcctgga cagcgtgaat cctgtcctcg gtaaggatgg
     4381 gttttccgca gtcgttacag taaaaagccg gtatcggcac tccccagatt cgctggcggc
     4441 tgatgcacca gtcggaacga tcacggacca tgttgaagat acggtccttc ccccagtcgg
     4501 ggatccagcg gacatcgttc tctatcgttt tgagcgcctc gttcctgaaa tcggctacag
     4561 caacaaacca ctgatctgtt gaacggaaaa tgacaggttt tttacaccgc cagcagtgcg
     4621 gatacgagtg ctggatcttg ctatttccca gaagacgccc attcgctgtc aggatttcga
     4681 agatcttttt tgagccttct tccagagaaa gcccgtcgac aagtccggcg ccttgaatga
     4741 agtgtccggt atcgtcgacc gggttataca cctctatgcc gaacttcacg cctgtttcat
     4801 agtcttccac gccgtgtccc ggggcggtat gcacacatcc ggtaccggtg tccagaagga
     4861 catagtcagc tggaagaaga ggaagagtac ggtcggggta gaacgggtgc accgcgtgca
     4921 actgttgcag cgcagctccc ttcacagtag ccagttcgtc ttcgaagaca ataccggtct
     4981 ccttcgccac gctcgcggca agggccttgg caaagacgta cactttcttc cctgcacgaa
     5041 cgaaaacata ctcgtgatcg ggtccaatag cgacagccat gctcgcgggg agcgtccaag
     5101 gcgttgtcgt ccatacaatg atattgacgt cttcgccggc gagcgacggc agaagcgcct
     5161 ccgcacccga cagggagtaa gccacgaaga ttgaagggga agtctcatcc cagtattcaa
     5221 tctccgccgc cgccagagcc gtctggcagt cgatgcacca gtagacgggc ttctttccct
     5281 tgtagacaag ccctttcccg acaagatcag caaaggcgcc gagctgggcc gcctcatact
     5341 ccggtcgcag cgtcatgtag gggttttccc agtctccaag cacgccgagt cgcacaaact
     5401 catctctttg tatatctcgg aactgaagtg catattcagt gcatttctcg cgcaaaagta
     5461 ctggatcgat cgtatccttt gacactccaa gactctttat aacgtgcagc tctataggca
     5521 ggccgtgcgt atcccaaccg ggaacatacg gcgaaaagaa ccctttcatc gccttgtatt
     5581 tcgggatgaa gtccttgagg attttattaa acgccgttcc aatgtgaata tgcccgttcg
     5641 cataaggagg cccgtcgtga agaacaaatg aaggagcccc ttcacgtttt ttcaacattt
     5701 tcccatagat gtcccgttcc ttccagaaat caagaaaccc aggctctctc ttcgcgaggt
     5761 tcgcccgcat cgggaaggcc gtttcgggaa gcgagagcgt gcttttgtag tccgtgctca
     5821 tgcgtgtacc cccttgtgga ataggaaaac cgtccctgaa aacaccggga cggtaaaaat
     5881 acttctatat actaacacat ttcttagcaa ccggccttat cctagggctg cttttctgga
     5941 ggagaatgac tgcaaaatgt agagcagcac cagaaccgct ataccgaaca gatcgctctg
     6001 ccacccggga ataagcagga aaagagcacc tgcaagggcg agcgcccgct tccacaaact
     6061 cagaactcct ctaaaatatc ccacagtgga catacccaat aaaacaacgc caagcattgc
     6121 agttccaaca gcaagtgcaa acggaacaaa gctataatcc acaagtacaa ggattgggtt
     6181 gaagacatag ataaagggaa caataaatcc agccaaggca agtttcacag ctgttacacc
     6241 agtcttcatc ggctctgctc ccgctattcc cgcgcctgcg tatgcggcga gggcaacggg
     6301 tggactgagg tcggcagcta tgccgaagta aagaacaaac atgtaggccg ccattggcgg
     6361 aacgccgagt tggaagagtg ccggagccgc catagtgctc gtaacaatga agttcgccgt
     6421 tgtcggcaga ccggctccga gaagaataca ggctaccatt gtcaaaagaa gcgtcaggag
     6481 aagattacct cctgccagag tgacaatcgc gctggcaata cgcaagccaa gacctgtaag
     6541 cgtggcagtc cccacgatga ttccaacggt ggcacaggcg cacgcaactc cgagagctga
     6601 acgggcgccg ctctcaagag ctgcgattaa acgcggaagc gtcaggcgag tctccttgtt
     6661 caaccaggaa acaacaaaag taatgatgat tccgttatac gccgctttca gcggagtata
     6721 tcctccgata aggaagtaga cgataccgac aagagggatc agcagatggc ctttttgacg
     6781 gagaagacgg agcaaaggag gaagctgatc cctcggaagc cctctgaggc ccaggcgggt
     6841 a
//
LOCUS       DAOPKU010000103         6827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11841_length_6827_cov_5.224749, whole genome shotgun sequence.
ACCESSION   DAOPKU010000103 DAOPKU010000000
VERSION     DAOPKU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11841_length_6827_cov_5.224749"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK849_04550"
     CDS             complement(<1..489)
                     /locus_tag="PK849_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE65427.1"
                     /translation="MNSAIEEMELTPRRIVEYLDRYIVGQEKAKKSVAIALRNRIRRR
                     NLSPEMAKEVAPKNILMVGPTGVGKTEIARRLAELVKAPFVKVEATKFTEVGYVGRDV
                     ESIIRDLAEAAVAMVKKRKIEEVQTPAAERAEQRLADALLPGSEKKPSMPDFMKIFGG
                     GRE"
     gene            complement(486..1019)
                     /gene="hslV"
                     /locus_tag="PK849_04555"
     CDS             complement(486..1019)
                     /gene="hslV"
                     /locus_tag="PK849_04555"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPE65428.1"
                     /translation="MFKGTTVICVRRGEQVAMAGDGQVTLGNQIIKAGARKVRRLYKG
                     SVLAGFAGSTADAMTLLERFESRLEENSGDLMRSAVAMVKEWRTDRALRRLEAMMIVA
                     DAKLSLLLSGAGDILEPENNIASIGSGSGFAQAAGMALLETSDWAPSKIAKRSIEIAS
                     EICIYTDSIVTVEAIGE"
     gene            complement(1012..1911)
                     /locus_tag="PK849_04560"
     CDS             complement(1012..1911)
                     /locus_tag="PK849_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPE65429.1"
                     /translation="MLENVWSTIDLFLDHLRFEKEASPHTVTNYAADLAQFAEFLDHQ
                     GVVRPESVDSRIVRFFLREIMGFGYSKTSVSRKLSAVRSWSAFLKMRGVLASDPAKGV
                     RGPKLPKLLPRALSREDAQLLVEDGPDGVDRVRDRAILELLYGCGLRIAELVSLRWEA
                     MEPVEERMLRVAGKGEKERIVPFGQCALAALREWRPFVPPDAPFLFPGLKGGAITVRT
                     VHRVVLRAAARVGLSGVTPHTLRHSFATHLLEGGATLAVVKELLGHESLLTTQRYLAI
                     NAEHLRESYRGAHPRAKGEDGLV"
     gene            complement(2076..3434)
                     /gene="trmFO"
                     /locus_tag="PK849_04565"
     CDS             complement(2076..3434)
                     /gene="trmFO"
                     /locus_tag="PK849_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPE65430.1"
                     /translation="MNRRVTVVGGGLAGSEAAWQLARRGIEVLLLEMRPLRTTPAHTT
                     EMLAEVVCSNSFGADGAASPAGILKNELRRLDSLILSCADESRVPAGKALAVDREIFS
                     QKVTERLESLPQVAVRREECVSLPEGPVIVATGPLTSPALAAILQRQAGTEYLSFFDA
                     VAPVVVADSIDMSVAFRRGRWGQEEDYINCPFTRERYEAFWNALVSAERALPHDFEDS
                     PSWFEGCLPVEVMASRGIDTLRFGPLRPVGLPLPGTGEEAWAVVQLRQDNREGTLFNL
                     VGFQTSLRWGEQERVFRMIPGLENAEFARLGVMHRNIYVNAPATLDKRLRFREGRTDL
                     FLAGQITGVEGYMESTAMGCVAALNAAALVDGVPFPEWPRATSIGSLLHYLSDAEPKH
                     FQPMNMNLGIMPKPEGKIRNKALRCEYVAEVASKAFDSFLESLKADSQTDDCQSEISG
                     ER"
     gene            complement(3427..5868)
                     /gene="topA"
                     /locus_tag="PK849_04570"
     CDS             complement(3427..5868)
                     /gene="topA"
                     /locus_tag="PK849_04570"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPE65431.1"
                     /translation="MVRKKIVTEAEVVESPSAPPKKGGKTAAKAEQRTAAPVKKAAPK
                     SASKKTLSSSAAKPKKTASSKTSAAKASSEKTLSADALPKKPVKKQAAKKSPASRPKT
                     KTALSSAAGSSDRTLVIVESPTKAKTLTKILGSKYTVKSSVGHIRDLPKSRLAIDIEN
                     DFAPEYILVKGKAKLKNELLAAAKQSPRVLLASDPDREGEAIAWHLAELLDIPLDTEC
                     RVRFYEITPTAVKEALAHPERVNMHKVEAQQARRILDRLVGYTLSPLLWKKIRRGLSA
                     GRVQSVALALICEREREIEAFVPKEYWIVTVLAVAEDGRVYSLKVDSFDGKSLWKEGK
                     SLLIDSEEFVDVLVGEILKAPLTVSDFKVRESYRAAPAPFKTSTLQQESARRNSMSPR
                     RTMSIAQELFEGVSLPDRGPTGLITYMRTDSLRIAPEALETCRQYIASAFDPEYLPEK
                     ANEYASKGRSQDAHEAIRPTDVTIVPKSLEGILTNDQMKLYSLIWNRYVASQMSKAKV
                     ANATLAATAGKVGLRQLGETLVFPGWSAVWPLDLKGESFAPARAGETLRVEKTEREQK
                     FTRPPARYSEASLIKVLEDEGVGRPSTYASIVETLYDRGYVARNEERRLQPSELGKTV
                     DSFLRKYFDAESLSAIVDTGFTARMEESLDDVEESNISWLAVLKEFWDAFTRTMQDAE
                     KAPAEKLPPPEPTGEDCPECGKPLVLKNGRFGDFIACSGYPDCKFTKPVLVKTGAVCP
                     KCGEGDVVKRKSRKGKPFYGCSRYPACDFVSWNPPSGETCPQCGAALFAKGTKGAEEC
                     MKCGFTRVRETAADE"
     gene            complement(5884..6210)
                     /locus_tag="PK849_04575"
     CDS             complement(5884..6210)
                     /locus_tag="PK849_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65432.1"
                     /translation="MPIIKKICGTRGRITESLEFKRVLHDLERRYLSPDAQAKIYRYL
                     ATEEVPLEVTERAILEAVALARLKNATADASLFDFLVDALLDDCSFEIPETASERVYP
                     SSCTLC"
     gene            complement(6242..>6827)
                     /locus_tag="PK849_04580"
     CDS             complement(6242..>6827)
                     /locus_tag="PK849_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPE65433.1"
                     /translation="VDVAYPRGHEPLFVRLAEEGGALLSEYPLGSQPKQWRFPERNRI
                     IASLADRLVVVEAPSKSGAMITARCALEAGREVWAVPGRINESGCEGSNRLLYDGAVP
                     LVSIPDFISLALGRQLSLFEETPFRGEQNSVAFTEKEQRILCLLEKSGERTVDNLAVE
                     GTMAPADVFSCLAALMAAGRVFVAGPGRWSAVPK"
BASE COUNT         1289 a         2155 c         2005 g         1378 t
ORIGIN      
        1 ctcccgtccg ccgccgaaga tcttcataaa gtcgggcatg gagggtttct tctcagatcc
       61 cggaagcagc gcgtcggcga gacgctgctc cgcccgctcc gccgccgggg tctgcacctc
      121 ttctatcttg cgtttcttga ccatggccac tgcggcctcg gcgagatctc tgatgatgga
      181 ctcgacgtcg cggcccacat accccacttc ggtaaacttt gtcgcctcca ccttgacgaa
      241 cggcgctttc accagctccg caagcctgcg ggctatctcg gtcttgccga cgccggtagg
      301 ccccaccatg aggatattct tcggcgccac ttctttcgcc atctcgggag agagatttct
      361 cctccggata cggtttcgca gcgcaatggc gaccgacttc tttgctttct cctggcccac
      421 gatgtaccgg tcgagatact cgacgatccg gcgcggcgtg agctccattt cttcaatggc
      481 gctgttcatt ctcctattgc ctccacagtc acaatgctgt ctgtataaat gcagatctcc
      541 gaagcgatct caatagagcg cttcgctatt ttcgacggcg cccaatcgga cgtttcgaga
      601 agcgccattc cggcggcctg cgcgaacccc gatccggagc cgattgaagc gatgttgttc
      661 tccggctcaa ggatatcgcc ggctccggaa agaagcagcg agagcttcgc gtccgcgacg
      721 atcatcatcg cttccagcct gcggagcgcc ctgtcggtac gccactcctt gaccatcgcc
      781 accgccgaac gcatcagatc gccggaattc tcctccaagc ggctttcaaa acgctccagg
      841 agcgtcatgg cgtcggcggt gctcccggcg aatcctgcga gcacgctccc tttgtagagt
      901 ctgcggacct tccgcgcgcc cgcctttatt atctgattcc ctaatgtgac ctggccgtcc
      961 ccggccattg ccacctgctc tcctcgtcga acacaaatca cggttgttcc tttaaacaag
     1021 cccgtcctct cctttcgcgc gggggtgggc gccacgatag ctctcccgaa gatgttccgc
     1081 attgatggca aggtagcgct gcgttgtcag caggctctcg tgccccagaa gttcctttac
     1141 gaccgcaagc gtcgcgcctc cctcaaggag atgcgtggca aagctgtgcc gcagcgtgtg
     1201 cggcgtcaca ccgctcaggc ccacccgagc cgccgcgcgg agcacaaccc tgtgcaccgt
     1261 ccggacggtg atggcgccgc ccttcaagcc gggaaagaga aacggcgcat cgggcggaac
     1321 gaacggacgc cattcgcgaa gcgcggcgag cgcacactgg ccgaacggga caatacgctc
     1381 cttctctccc ttgcctgcaa cgcgaagcat tcgttcctcg accggctcca tggcctccca
     1441 tcgaagcgag acaagctctg cgatccgaag accgcaaccg tagagcagct ccaaaatcgc
     1501 cctgtcgcgg acccggtcga cgccatccgg cccgtcttcg accagaagct gcgcgtcctc
     1561 gcgggaaagc gccctgggca ggagcttcgg aagcttcggc ccccgtactc ccttcgcggg
     1621 gtcggaagcc agcacgccgc gcattttcaa gaacgccgac cagcttcgca cagcggagag
     1681 cttcctggag acggaggttt ttgagtagcc gaaccccatg atctcccgaa gaaaaaagcg
     1741 tactatccgg gaatcaacgc tctccgggcg cacaactccc tggtggtcaa ggaactccgc
     1801 aaactgagcc agatccgcag cgtagttggt caccgtatgc ggcgaggctt ctttttcaaa
     1861 tcgcaggtga tcaagaaaaa gatctatggt tgaccagacg ttttccaaca tagagacatt
     1921 ttatcacgtt ttctagattc tcgctatttt tcaaaagaat caattaaggc atgttttctt
     1981 ttgcgcaaaa gcgcatgaac agcgcattga gcgccgtaca ttactctgaa gaacatattt
     2041 ttatacaaag agaattatga gaaaaaacaa gtcgtttacc gctcaccgga aatttcagac
     2101 tggcagtcgt ccgtctgaga atccgccttc aatgattcaa gaaaagaatc gaaagccttg
     2161 gatgcgacct cggcgacgta ttcgcaccgg agcgccttgt tccgaatctt tccctccggt
     2221 ttcggcatga tgccgaggtt catgttcatc ggctggaagt gcttcggctc cgcgtcactc
     2281 aaatagtgca gcagcgaacc gattgaggtc gccctcggcc actccggaaa cggaacgccg
     2341 tcgacaagcg ccgccgcgtt gagcgccgca acgcacccca tcgcggtgct ctccatgtac
     2401 ccctcgacgc cggtgatctg tcccgcgagg aagagatccg ttctgccttc gcggaagcgc
     2461 aggcgcttgt cgagcgtcgc cggggcattg acgtagatgt tcctgtgcat aacgccaaga
     2521 cgggcgaatt cggcgttttc aagcccgggg atcatccgga agacgcgctc ctgctccccc
     2581 caccggaggc tcgtctgaaa gccgacgaga ttaaagagcg tcccttcacg gttgtcctga
     2641 cgaagctgca cgacagccca tgcctcttcc cccgtgccgg gcagaggaag ccccaccggc
     2701 cgcagcggac cgaagcggag ggtatcgata ccgcgcgaag ccatcacctc gacggggaga
     2761 cacccctcga accaggatgg agaatcctcg aagtcgtgcg gaagcgcgcg ctctgcggag
     2821 acgagcgcat tccagaacgc ctcgtaccgt tcgcgcgtaa aggggcagtt gatatagtcc
     2881 tcctcctgcc cccatcgtcc ccgccggaag gcgacggaca tgtcgataga gtcggcgacg
     2941 acgacggggg cgacggcgtc gaagaaggag aggtactccg ttccagcctg tcgctgcaag
     3001 atcgcggcaa gcgccgggga tgtcagcggc cccgtggcga cgatcaccgg tccttcgggc
     3061 aaggagacgc actcctccct gcgaacggcg acctgcggaa gcgattcgag ccgttccgtg
     3121 accttttgcg aaaagatttc cctgtcgacg gcgagcgcct tgcccgcagg aacgcgagat
     3181 tcgtcggcgc aggagaggat aaggctgtcc agacggcgca gctcgttctt gaggattccg
     3241 gcggggctcg cggcgccgtc cgcgccgaag gagttgctgc agacgacttc ggcgagcatc
     3301 tccgtggtgt gcgccggagt cgtccgaagg gggcgcattt cgagcagcaa tacctcaata
     3361 ccgcgccgcg caagctgcca cgcggcctcc gaaccggcga gtccgcctcc gacgacggtg
     3421 acgcgcctat tcatccgcag ccgtctcccg tacccgggta aaaccgcact tcatgcactc
     3481 ctccgcgccc ttcgttccct tggcgaaaag agccgcgccg cactgcggac acgtctcccc
     3541 cgagggggga ttccaggaga cgaagtcgca cgccgggtag cgcgaacagc cgtagaaggg
     3601 cttccccttg cggctcttac gcttgacgac atctccctcg ccgcacttcg gacagaccgc
     3661 cccggtcttc acaaggaccg gtttggtgaa tttacagtcg gggtagccgc tgcatgcgat
     3721 gaaatcgccg aaacggccgt tcttgagcac gagcggcttg ccgcactcgg gacagtcttc
     3781 gccggtaggt tccgggggcg gaagcttttc ggccggggcc ttctccgcgt cctgcatcgt
     3841 gcgggtaaag gcgtcccaga actctttgag gacggcgagc caggagatat tgctctcctc
     3901 gacgtcgtcc agactctcct ccatgcgggc ggtaaagcct gtgtcgacga tcgcggagag
     3961 gctctccgcg tcgaagtatt tcctaaggaa ggagtccacc gttttcccga gctcggaggg
     4021 ctgaaggcgg cgctcttcgt tgcgcgcgac gtaccctctg tcgtagagcg tctccacgat
     4081 ggatgcgtac gtcgagggac ggccgacgcc ttcgtcctcg aggaccttga tcaggctggc
     4141 ctcggagtag cgtgcgggcg ggcgcgtgaa tttctgttcg cgctccgtct tctcgacgcg
     4201 aagcgtctcc ccggcgcggg ccggagcgaa gctctccccc ttgaggtcga gcggccagac
     4261 ggcgctccac cccggaaaga cgagcgtctc gccgagctgg cgcaacccga ccttgccggc
     4321 ggtcgcggcg agcgtcgcgt tggcgacctt tgccttcgac atctgcgagg ccacgtagcg
     4381 gttccagatg agcgagtaga gcttcatctg atcgttggtg aggatgcctt cgagactctt
     4441 cggcacaatg gtcacatcgg tcggccggat cgcctcgtgc gcgtcctggg agcgtccttt
     4501 ggacgcgtat tcgttcgctt tttccggaag gtactccgga tcgaaggcgg aggcgatgta
     4561 ctgtctgcac gtctcaagcg cctcgggagc gatcctgagg ctgtcggtgc gcatgtaggt
     4621 gatgaggccg gtcggccccc tgtcgggcag agagacgcct tcgaaaagct cttgggcgat
     4681 gctcattgtc cgccggggcg acatgctgtt cctgcgggcc gattcctgct gtagggtgct
     4741 cgttttaaaa ggagccgggg cggcgcggta gctttcgcgc accttgaagt ctgaaactgt
     4801 gaggggagct ttcaggatct cgccgacgag gacgtccaca aactcctccg agtcgatgag
     4861 cagggacttc ccttccttcc agaggctctt cccgtcgaag ctgtccacct tgagcgaata
     4921 gacccttccg tcctcggcaa cagcgagcac tgtgacaatc cagtactcct ttggaacaaa
     4981 cgcttctatc tcgcgctccc gctcgcagat aagggcgagc gcaacagact ggaccctccc
     5041 tgcggagagc cccctgcgta tcttcttcca gagaagcgga ctcagcgtat accccaccag
     5101 ccggtcgaga atgcgccgcg cctgctgcgc ctccaccttg tgcatattga cgcgctcggg
     5161 gtgcgcaagc gcctctttca ccgccgtggg tgtgatttca tagaaacgga cgcggcattc
     5221 cgtgtcgagc gggatgtcga gcagttccgc aagatgccac gcgatcgcct cgccttcgcg
     5281 gtcggggtcg gaggcgagca gaacgcgcgg agactgcttc gccgcggcga gcagctcgtt
     5341 cttcagcttc gccttcccct tgacgaggat gtactcgggg gcgaagtcat tctcgatatc
     5401 gatggccagc ctgctcttcg gcagatcgcg aatgtggccg acgctcgact tcaccgtgta
     5461 cttcgagcca aggatcttcg tgagcgtctt cgccttcgtg ggagactcca cgatgacgag
     5521 cgtcctgtcc gaagagcctg ccgcggaaga aagcgctgtc ttcgtcttcg gccgggaagc
     5581 cggactcttt ttcgccgctt gttttttcac gggtttcttc gggagcgcgt ccgctgaaag
     5641 cgtcttctcg gaagacgctt tcgcagcgga ggtcttcgac gaggccgtct tcttcggttt
     5701 cgccgcagaa gacgaaagtg tcttttttga agcgcttttc ggggcggcct tcttcacggg
     5761 cgccgcggtc ctttgttcag ccttcgcggc cgtcttcccg cccttcttcg gcggagcgga
     5821 gggactctct acaacctccg cttcagttac gatctttttg cgaaccatgc gaaaaaaccg
     5881 tcctcagcaa agggtgcagc tcgaaggata gaccctctcc gaggccgtct cggggatctc
     5941 gaaggaacag tcgtcgagca gcgcgtctac gaggaaatca aaaagagaag cgtccgcagt
     6001 ggcgttcttg agacgggcaa gagcgaccgc ttcaagaatc gcccgctccg tgacctccag
     6061 cggcacttcc tctgtagcaa gatagcggta aatcttcgcc tgtgcgtcag gggagagata
     6121 cctccgttcc agatcatgga gcactctttt gaactcgaga gactcagtga tgcgcccccg
     6181 ggttccgcaa atttttttta taatgggcac caggtttctt cctttcccga atggtttgga
     6241 gttacttcgg gacggcgctc caacggcccg gtcccgctac aaaaacacgt cccgcagcca
     6301 taagcgcggc gagacaggaa aatacatcag cgggagccat tgtaccctca acagcaaggt
     6361 tgtcaacagt tctctcccct gatttttcca aaaggcaaag aattctttgc tctttttcag
     6421 taaaggcaac ggaattttgc tctccccgga acggcgtctc ttcgaagagg gagagctggc
     6481 gtccgagcgc gagggaaatg aagtcgggta tcgacaccag cggtacggcg ccgtcgtaca
     6541 ggaggcggtt ggaaccttcg caccccgatt cgttgatcct tcccgggacc gcccatactt
     6601 cccgtcccgc ttcgagggcg cagcgggcag taatcatggc gccgctcttc gaaggcgcct
     6661 cgacgacgac cagacggtcg gcgagcgacg caatgatgcg gttgcgctcc ggaaagcgcc
     6721 actgcttggg ctgcgaaccg agggggtatt ccgagagcag cgcgcctccc tcttcggcga
     6781 ggcgaacaaa gagcggttcg tgaccgcgag ggtatgcgac gtcgacg
//
LOCUS       DAOPKU010000104         6792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11908_length_6792_cov_4.690515, whole genome shotgun sequence.
ACCESSION   DAOPKU010000104 DAOPKU010000000
VERSION     DAOPKU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11908_length_6792_cov_4.690515"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..858
                     /locus_tag="PK849_04585"
     CDS             295..858
                     /locus_tag="PK849_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPE65434.1"
                     /translation="MSFVEMLLSAASLSMDAFAASLGIGACLGAGASAAAFRVGAACG
                     GFQFAMPLAGWWLGSRFLESISGYDHWIAFFLLLLVGGNMIREAFTKDEPNCPTADPS
                     CGFALLFVALATSIDALAVGIGFAAIGAPVMPLSSSAGIITALLSFGGVLAGFRTALF
                     LGRKAEFAGGVVLCIIGANILRMHLAA"
     gene            861..1127
                     /locus_tag="PK849_04590"
     CDS             861..1127
                     /locus_tag="PK849_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPE65435.1"
                     /translation="MEAWVYILRCADGTLYTGWTFDTEARLCVHNSGKGARYTASRLP
                     VEMVYRERVASKSDALRRERRIKRMRRSAKERLIAGACSVSLPE"
     gene            1254..2774
                     /locus_tag="PK849_04595"
     CDS             1254..2774
                     /locus_tag="PK849_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPE65436.1"
                     /translation="MSDMRMANIDKLTLEIEKLLADAEVTGALPQVEDRDASWEVRIS
                     DDGLTAYLDMYPAVGNGVSPDPLGICTELEEMGIRRVDRDRVMALVTLCDSGYPASGE
                     DAVVARGVPPESPSPGRVEFLVLMERAKRSEDDEASVDWKNLWLTPSVHEGDVIAKIY
                     PPKEGTDGVDVYGNPLPAASHTFFRIRYGDGVNVSEGEDGTVEIASARTVGQPVFRDN
                     VLDVAPLLVINGDVNISTGSIDFTGSVLVSGSVLEGFSVKAERDVNVQGWVYNARVQA
                     GGSCSVKGGVTGERSLLLAGEDVRVGVVEYAEISASRKVEVFGYALFAMLEAGESIYV
                     QGRNRRGVVGGTCIAGACIEALSAGSPMESSTQLEAGRDPFQARILQELDRKKEALLA
                     MQKKLEQAILSIKGTGPNFDIDTLTEEEKGKLFLLVQYHGKMKSSVAELAARIDDEKR
                     ASIAEKRIMPRIRIRDRVYPNVTLKIWGQTLVVKQQEFHVSFFMDKDRGVIARGGF"
     gene            2806..4431
                     /locus_tag="PK849_04600"
     CDS             2806..4431
                     /locus_tag="PK849_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE65437.1"
                     /translation="MEKWNEELLKSIESIASEAGVRPDGKDSGTMEGLKPEELHSNDI
                     VFRSTSRAVVVCRMLFNARGIPVDFLLESVNPAFCRIFGIAPESAAGRSGKEFFMPDG
                     KEIPALDLFDNALKEQQAFSFMFSSEKLSKVFDVLAFSLENGTFCMAITDATSQVAAE
                     RERRENERFTEKVFGSVDVWIVLLDERGRVVFWNAAAERLSGISADEALSGSFDFRSV
                     FCPGEEDREDAIGKMDACRRDGATEVRLETCVCAAEGRSLRVEWTVRPWHFEDVSSDG
                     LLLLGRDVTQDRHTEEQLLESERRYAAAFKETMAPMLLVDPETGAVCDCNAAALALYG
                     YSREEMLALTNRDFNLLPAEDLRKNMEEATARLRNRFVFQHRLKNGDIRTVEVFASPT
                     PFQGKTFLFSILHDITEKQRLEEQLHLVTFDCALQAKILGGILESSPDYLYVFDRMRK
                     FCFSGKKGAALFERTPEEMTGRSWEALGIPEERIAAFDEHLAQTFAAGKSKRGVLVFP
                     APHGDMPLEYALYPICSENGVVDMVFCSMHAVP"
     gene            4464..5984
                     /locus_tag="PK849_04605"
     CDS             4464..5984
                     /locus_tag="PK849_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPE65438.1"
                     /translation="MDELRIANLERLVTEIDHFLSGTKETGALPAAESRDASWELRVS
                     DDRLSAWLETYPQVGAGIPLDAAEIIEALEEAGLCLLDPGKIRAIIEKCNKGEEVRGT
                     EALVASGTATVDPVEGKIEFLVPFEKVKLVDEADERAIDWKNLWSIPIVHTGDIIARV
                     HPPQEGVEGTDIYGQPLPPRRPAPFRVKFGEGVVVSEEDGMTEVLTARDVGQPVFRGG
                     ELDVLPVLEVPGDVDILSGDIDFIGSVVVRGSVTEGFSVKAGRDVAVSGSAFNARIEA
                     SGSCIVEGGIVGEHCEIRAGEDIRAGFVEYGLLISGVNIEILGYTLFATLEARQSVLV
                     QGKNRRGIIGGVTTAGTTIKALSAGSAMEPRTLLEAGRDAVRNRNLAALTAKMAELEA
                     MKTKIEQAMLSIKRPGLGFDLEHLSEEEKGKLFLLARYHKKVGENMAELAGKIEDEKR
                     AILAERKGTPLIKIRDKIYANVTLKIWESQLTVSSAEFCAGYYFDRKNGSIQKGLY"
     gene            6016..>6792
                     /locus_tag="PK849_04610"
     CDS             6016..>6792
                     /locus_tag="PK849_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE65439.1"
                     /translation="MENWDDSLLDQLEELNEIVRKAEEESAAAEIFTENGEHALCKAV
                     LWSMEQAVVVLSLLYDESGEAEDCICEKVNPAFERVSGISEERAVGSKGSEIFGEKIP
                     YFDIFVRVAETKSKVRFKAHYPKIGKYFDMWIFSPEWGRIALVFSDVTDSVLAEREQQ
                     LDRRFLDEILDSMNVWLMIFDNRWNPILWNKAAERLSGYCSEEALSPGSRVLEKLFPE
                     ELYREYVLEEMKKAAHRPEGASGELETEVVVNGGRTRTIAW"
BASE COUNT         1408 a         1768 c         2210 g         1406 t
ORIGIN      
        1 gttgctgaag tgggacgaaa cgggaaaggc gaccatttat aggccgtaaa caggctgtac
       61 cctgtgaaag gtgttgtagg cgttcgggag tcagagagaa cgctctgttc tgcggcgtgc
      121 tgcaaccaac ggtgcgacga agggaaagca tctgtcgcgt gcggatgaga atggagctgc
      181 cgaacccatt gatctcgttt caagaatcgg ctctgtgaaa ataaaaaaac tggcctttga
      241 catcttgtgc gtatggagaa ggtctttttt tagtgaagaa aggtatgatg cttcgtgtcg
      301 tttgttgaaa tgctgctttc cgccgcgtcg ctttccatgg acgcttttgc cgcctccctg
      361 ggtatcggcg cgtgcctggg cgccggagcc tcggccgcgg cattccgcgt cggggctgcg
      421 tgcggagggt ttcagttcgc catgccgctt gcgggttggt ggctggggag ccgttttttg
      481 gagtccatat cggggtatga tcactggatt gcctttttcc tgctgcttct cgtcggagga
      541 aacatgatcc gggaggcctt cacgaaggat gaaccgaact gtccgacggc ggatccctca
      601 tgcggttttg cactcctttt tgtggcgctg gcgacctcca tcgatgcact cgccgtgggc
      661 atcggttttg ccgccatcgg agcccctgtc atgccgctct cttcgtccgc gggtatcatt
      721 acggcgctgc tctcttttgg cggggtgctc gcaggcttcc gcacggccct cttcctcggc
      781 cgcaaggcgg agttcgcggg cggcgtcgtg ctctgcatca tcggcgccaa tatcctgagg
      841 atgcacctgg cggcgtaacc gtggaggcgt gggtctacat cctccgctgc gccgacggga
      901 cgctctacac cggctggacc ttcgatacgg aagctcggct ctgcgtccat aacagcggca
      961 agggcgcgcg gtacaccgca tcgcgccttc cggtcgaaat ggtgtacagg gaacgcgtgg
     1021 cctcaaagag cgatgcgctg cgcagagagc ggcggatcaa gaggatgcgc cgcagcgcga
     1081 aagagcggct tattgccggc gcctgctcgg tgagtctccc ggaatagtcc ttgttattct
     1141 tcttcgcaaa atacccagcg gttcgaaccc tcttcttgcc cttccggaga aaaaagagta
     1201 ttctgaagct gggtatgctc ttgtgttgtg gaaaatgaga gggggtcttc cgtatgagcg
     1261 atatgcgaat ggccaacatc gacaaactga ccttggaaat cgagaagctt ctcgccgacg
     1321 cggaggtaac aggagctctc cctcaggtgg aggacaggga tgcgtcgtgg gaggtgcgta
     1381 tttctgacga tggcttgaca gcgtatctcg acatgtaccc cgcagtcggg aacggcgttt
     1441 cccccgatcc gctcggaatc tgcacggagc ttgaagagat gggaatacgc cgagtcgacc
     1501 gcgacagagt gatggcgctg gttactctgt gcgattccgg atacccggcc tcaggggagg
     1561 acgccgtcgt cgcgagaggt gttccgccgg agtctccgag cccgggccgg gtcgagtttc
     1621 tggtcctcat ggaaagggcg aagcgctccg aggacgacga ggcgtccgtc gactggaaaa
     1681 atctctggct caccccctcc gtgcacgaag gagacgtcat cgcgaagatt tatcctccga
     1741 aagagggaac cgacggggtg gatgtctacg ggaatcctct tcctgctgct tcacacacgt
     1801 ttttccgcat tcgttacggc gacggtgtga acgtctcgga gggtgaagac ggcacggttg
     1861 aaatcgcatc ggcacggacg gtcggccaac ccgtcttccg ggataatgtg ctggatgtgg
     1921 cgcctctgct ggtcatcaac ggcgacgtaa acatctccac cggcagcatt gattttaccg
     1981 gctccgtcct tgtctccggt tcggtgctcg aaggtttttc cgtcaaggcg gagagagacg
     2041 tcaatgtgca ggggtgggtg tataacgcgc gagtgcaggc ggggggctcg tgcagtgtaa
     2101 aagggggcgt gacgggagaa cgctcgttgc ttctcgcagg cgaagacgtg cgcgtcggcg
     2161 ttgttgaata cgcggaaata agcgcctcac ggaaggtcga agtcttcggc tacgcgctct
     2221 ttgcgatgct tgaggcgggc gaatcgatct acgtccaggg gaggaaccgc aggggcgtcg
     2281 tgggcgggac ctgtatcgcc ggcgcctgca tcgaggcgct ctccgcaggc tctccgatgg
     2341 agtcgtccac gcagcttgag gcgggacgag acccgttcca ggccagaata ctccaggagc
     2401 ttgaccggaa gaaagaggcg ctccttgcga tgcagaagaa gctagagcaa gcgattcttt
     2461 caattaaagg aacaggcccg aattttgata ttgacactct taccgaggag gaaaagggca
     2521 agctttttct cctcgttcaa taccacggca agatgaagag ttccgtagcg gagctggccg
     2581 caaggatcga cgatgaaaaa cgcgcgtcca tagcggaaaa gcgcatcatg ccccgcatcc
     2641 gcatccgcga cagagtctac cccaacgtca ccctgaaaat ttggggacaa acgctggtgg
     2701 tcaagcagca ggaattccat gtgtctttct ttatggacaa agaccgcggc gtgatcgcaa
     2761 gaggcggttt ctgagagcgg gaacagccgg aaggaggagt gcgtgatgga gaagtggaac
     2821 gaggagctgc tgaagagcat cgagtcgata gcctccgaag ccggagtgcg tcccgacggg
     2881 aaagactcag gaactatgga gggtttgaag cctgaagagt tgcattcaaa cgatatcgtt
     2941 ttccgttcta cctcacgcgc agtggttgtc tgccgtatgc tcttcaatgc gcgggggata
     3001 ccggtagatt ttctgctgga gagcgtcaac cccgcttttt gcaggatttt cggcatagcc
     3061 ccggagagcg cggcgggcag gagcggaaag gagttcttca tgcccgatgg caaggagatt
     3121 cccgccctgg acctttttga caatgctctc aaagaacaac aggccttttc tttcatgttt
     3181 tcctcagaaa aattgagcaa ggtttttgac gttcttgcgt tctctctcga aaacggcacg
     3241 ttttgtatgg ccataacgga tgcgaccagt caggttgccg cggaaaggga gcggcgggag
     3301 aacgaacggt ttacggaaaa agtcttcgga agcgtggatg tctggatcgt gctgctcgac
     3361 gaacggggaa gagtcgtctt ctggaacgcc gccgcggagc gcttgtccgg catctcggcc
     3421 gacgaagcgc tctcggggag ctttgatttc cggagcgttt tttgtcccgg agaagaggat
     3481 cgtgaagatg ccatcgggaa gatggatgcc tgccgaaggg acggtgcgac ggaggtccgg
     3541 ctggaaacct gcgtgtgcgc ggctgaggga agatcgcttc gtgtggagtg gacggttcgg
     3601 ccgtggcatt tcgaggatgt ctcctccgac ggtcttctgc tccttgggcg cgatgtgacg
     3661 caggatcgcc acaccgagga gcagcttttg gaaagcgagc gccggtatgc cgccgccttc
     3721 aaggagacca tggcccctat gctcctcgtc gacccggaaa cgggcgccgt atgcgactgt
     3781 aacgccgccg cccttgccct ctacggttat tccagagaag agatgctcgc cttgacgaac
     3841 cgagatttta atctgcttcc ggcggaagat ctgcgcaaga acatggaaga ggcgacggcg
     3901 cggctcagga accggttcgt tttccagcat cgtctcaaga acggcgacat ccgcacagtg
     3961 gaggtctttg cttcgccgac tccgtttcag gggaagacct ttctcttttc tattcttcac
     4021 gatatcaccg agaaacagcg tctcgaggaa cagctccatc tggtcacctt cgactgcgcc
     4081 ctgcaggcga agattctcgg cggtattctg gagagctcgc ccgactacct ctacgtgttt
     4141 gatcgcatga gaaaattctg tttttccgga aagaaagggg cggcgctctt tgaaagaaca
     4201 ccggaggaga tgaccggtcg ttcatgggag gcgctcggca ttccggagga gcgaattgcc
     4261 gcgttcgatg aacatttggc gcagacgttc gcagccggga aatcgaagcg cggcgttctt
     4321 gtttttccgg cgccgcacgg cgacatgccg ctggagtacg cgctctaccc catttgcagt
     4381 gaaaacggcg tcgtcgacat ggtcttttgc tcgatgcatg ccgttccgta ggtcggagcg
     4441 ccggagcggg aaggagaaaa cgcatggacg aactgcggat agccaacctg gagagactgg
     4501 tgacggagat cgaccatttt ctctccggga cgaaggagac aggcgcgttg cccgcggcgg
     4561 agagccgcga cgcgtcatgg gagctcaggg tctccgatga tcgtctgtcc gcatggctcg
     4621 aaacctatcc ccaggtcggc gccgggattc ctctggacgc tgcggagatt atcgaagctc
     4681 tggaggaggc ggggctttgt ttgctcgatc cgggaaaaat ccgtgcgatc attgaaaaat
     4741 gcaacaaagg cgaagaagtg agggggacgg aggccttggt cgcctccgga acagcaacgg
     4801 tcgatcccgt ggaagggaag atcgagttcc tcgtgccctt cgaaaaggtc aaactggtcg
     4861 acgaagccga cgagcgcgcc atcgactgga aaaacttgtg gagtattccg atcgtccata
     4921 ccggcgacat catcgcccgg gttcaccccc cgcaggaggg cgttgaaggg acggacatct
     4981 acggtcagcc gcttcctccc cgccgccccg cgcccttccg ggtgaagttc ggggagggcg
     5041 tggtcgtttc ggaggaagac ggcatgaccg aagtgcttac cgcccgggac gtggggcagc
     5101 ccgtcttccg cggcggcgag ctggatgtgc tccccgtgct tgaggtaccc ggcgacgtcg
     5161 acatactttc cggcgatatc gacttcatcg gctcggtggt cgtccgcggt tcggtgacgg
     5221 aggggttttc cgtgaaggcg gggcgcgatg tcgccgtatc gggttccgcc ttcaacgcac
     5281 gtatcgaggc ttcggggagc tgcatcgtcg agggtgggat cgtcggcgaa cattgtgaaa
     5341 tcagggcggg cgaggatatc cgcgcggggt tcgtggagta cggtctgctt atttccgggg
     5401 tgaacatcga gattttgggc tataccctct tcgccacgct cgaagcgagg cagtccgttc
     5461 ttgtgcaggg gaagaaccgg cgcggcatta tcggcggcgt tacgaccgca ggaacgacga
     5521 tcaaggcgct ctccgcaggt tccgccatgg aaccgaggac tttgctcgaa gcggggcgcg
     5581 atgcggtcag aaacagaaat ttagcagcat tgacggcgaa gatggcggag cttgaagcaa
     5641 tgaaaacgaa gatcgaacag gccatgctct cgataaagcg cccgggtctc ggcttcgacc
     5701 tggagcatct ttccgaggag gagaagggga agctttttct cctggcgcgc taccataaga
     5761 aggtcggcga gaatatggcc gagcttgcgg ggaagatcga ggatgaaaag cgggcgatac
     5821 tcgccgaaag aaaaggaacg ccgctcataa aaatccggga caagatttac gccaacgtta
     5881 cgctgaagat atgggaatcg cagctgacgg tctcttcggc ggaattttgc gcgggttact
     5941 atttcgaccg gaagaacgga agcatacaga aggggctgta ctgagccggg tttcggcgga
     6001 gaggggttgg atagggtgga aaattgggac gacagccttt tggaccagtt ggaagagctg
     6061 aacgagatcg tccggaaagc tgaagaagaa agcgccgccg ccgagatttt tacagagaac
     6121 ggcgaacacg ctctctgtaa agcggtcctc tggagcatgg aacaggcggt tgtcgtgctt
     6181 agccttctgt acgatgaatc cggcgaagcg gaggattgca tctgtgaaaa ggtcaacccc
     6241 gctttcgaac gtgtttccgg tatttccgag gaacgagccg taggcagcaa ggggagcgag
     6301 atattcggag agaagatccc gtatttcgac atctttgtcc gcgttgccga gactaaaagc
     6361 aaggtacggt tcaaagcgca ctacccgaag atcggcaagt acttcgacat gtggattttc
     6421 tcccccgagt gggggaggat cgcgctcgtc ttcagcgacg tgacggattc ggtgctggcg
     6481 gagcgggagc agcagctcga ccggcgtttc ctcgacgaga tactggacag catgaacgtc
     6541 tggctgatga tcttcgacaa tcgctggaat ccaatactct ggaacaaggc ggcagaacgg
     6601 ctgagcggct actgcagcga agaggccctg tctcccggtt cccgcgttct ggagaagctc
     6661 ttcccggagg agctgtaccg tgagtacgtg cttgaagaga tgaagaaggc cgcacaccgg
     6721 ccggaggggg cttccgggga gctggagacc gaggtcgtgg tgaacggcgg ccggacccgc
     6781 acgatcgcat gg
//
LOCUS       DAOPKU010000105         6782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_11930_length_6782_cov_3.973391, whole genome shotgun sequence.
ACCESSION   DAOPKU010000105 DAOPKU010000000
VERSION     DAOPKU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11930_length_6782_cov_3.973391"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1474)
                     /locus_tag="PK849_04615"
     CDS             complement(236..1474)
                     /locus_tag="PK849_04615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE65440.1"
                     /translation="MSEMLKKYGEVVGFHAMDSLMVIADMLRGKKIVHVNSTKEGGGV
                     AEILSSMVPLSKELGLDVSWEVIEGNPDFFACTKTLHNSLQGMPGGLPEQQIEAYVET
                     NRTNAEKLRGVVEDADYVFIHDPQPAPLLGYFPKRKGKWIWRCHIDVSSPNRSVWRFV
                     KKHVEGYDASIFSLPDFAQTLPHPQFLITPSIDPLSEKNIALPKSEVAKTYEELGIPM
                     DKPVILQVSRFDRFKDPLGVIESFRIAREHVDIRLVLAGGGATDDPESGEVLARVQEA
                     AAGDPDIHVLLLPADAHRTINALQSGADVVMQKSTKEGFGLTVTEGLWKKKPVIGGDV
                     GGIRLQIINRFNGFRVRSPEGAAIRIRYLLTHKWKRERMGVNARQYVLENFLITGQLR
                     RYLSLLVSLNSPDGEWERWI"
     gene            complement(1477..2151)
                     /locus_tag="PK849_04620"
     CDS             complement(1477..2151)
                     /locus_tag="PK849_04620"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HPE65441.1"
                     /translation="MKEHGTTLPFRFMECALLTLSTGVRAQSIRELRTALPQTPLSSI
                     YYHFWGRMLRPHIAESEFNNDFASWADSGLGDIELAEALSAINPVACADLGEIRSILE
                     DILDARLENGDFMSWKKSDREFYFIACTKMMFDTGREVSSLKDFPLAVKTASRQSFFH
                     HFIDGRRRSPEGKDDFSLWLEQFGEETEPLRKSLRAIDSYLFSLGELKGQVVSLLEKN
                     LHRRGK"
     gene            complement(2329..3198)
                     /gene="secF"
                     /locus_tag="PK849_04625"
     CDS             complement(2329..3198)
                     /gene="secF"
                     /locus_tag="PK849_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPE65442.1"
                     /translation="MKIMFIDFMKHRRAALLVSLVCVVVSIGLVFTKGLNLGIDFTGG
                     NVVQVEFAQGVSVGDVRDVLASVGQGNAVIQSYSDIGFIIRISANEDEARKMVVDALR
                     AKYPDMKTVRLEKVGPVVGAELRKEAFVALFIALAGILAYITVRFQFRFAVVSVLTLM
                     HDAIITLGVFSLTGMEISSSFIAAILTIVGYSLNDTIVVLDRVRENWKTLRGNGMLAL
                     LNNSINETISRTINTSMTTLLPVIALFLWGGPVLRSFSFALLIGITVGTYSSIYIASG
                     VLAEWWIKAPQKA"
     gene            complement(3218..4612)
                     /gene="secD"
                     /locus_tag="PK849_04630"
     CDS             complement(3218..4612)
                     /gene="secD"
                     /locus_tag="PK849_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPE65443.1"
                     /translation="MLRRDRWRLGIVAIVVLAALFSVFPIQGRINLGLDLKGGAHIVL
                     QAKDTADSKVTEDSIERLLAVLRNRVDQYGVAEPVIQREGTDRIIVDLPGIQDPDAAL
                     ELIGKTALLEFRQVVTATSPVPPSAERKNYDSDAEFERAQTRWNEAVAKIEAQKGELE
                     QQAASLEGGVVAKADEGRFYLLGKVLVGGKDLVDARTQYDNLGRAVVAISFSSEGAKL
                     FDAATAENIGRQIAIVLDGVVISAPVVQERITGGNAQISGRFTAAEASRLSIMLRAGA
                     LPVAVDVIENRSVGPSLGEDSINSGIRAGLIGASLVFLFMLLYYRLLGVAADAALAVA
                     ILLVFAGLISLKATLTLPGIAGIVLTIGMAVDGNVLIYERMKEELRAGKTPLAAIDAG
                     FKKALVTIFDANITTLIAAAVLFYFGSGPVRGFAVTLSIGIVSSVFCNVLVTRSLLQY
                     FMGGRATSSLAHRS"
     gene            complement(4803..5162)
                     /gene="yajC"
                     /locus_tag="PK849_04635"
     CDS             complement(4803..5162)
                     /gene="yajC"
                     /locus_tag="PK849_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPE65444.1"
                     /translation="MEQQCGQAAGGAGGQGSLMGMLFPLAIFVVIFYFFIIRPQKKRQ
                     RAQDELLSSLSRGDQVVTIGGFFGTIREVRDDSLVVEIADGVKVRLLKSAVASKRAPA
                     TQAPKEEPAREKEEEKS"
     gene            complement(5254..5889)
                     /locus_tag="PK849_04640"
     CDS             complement(5254..5889)
                     /locus_tag="PK849_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPE65445.1"
                     /translation="MKDTKIAEPTVERLVQYHRLLEQLAAEGQKVVSSREMGEMLSFK
                     ASQVRKDLSYFGEIGKRGVGYHVDRLFKHIGGILASPRLWSIGLAGVGKLGEALLGYQ
                     AFRNEKFQIKALFDVDPSKIGTHIQGTPCYSIEDAPRVLREEGIDVVIITVPTGAAQS
                     VADRVVSAGTVKGILNFSPVTLSVPDSVLLYSVDISIELEKLLFYLKHRER"
     gene            complement(5945..6325)
                     /locus_tag="PK849_04645"
     CDS             complement(5945..6325)
                     /locus_tag="PK849_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPE65446.1"
                     /translation="MKGIVSTTNAPAAVGPYSQAVKAGQFLFCSGQIPIDPATGQFVP
                     GGVAEQAKQVLNNVKAVLEAAGYSLADVIKATVFATSMNDFAAVNGVYATYFEKEPPA
                     RSFVEVSALPKGALVEIEVVAWKE"
     gene            complement(6356..>6782)
                     /gene="eno"
                     /locus_tag="PK849_04650"
     CDS             complement(6356..>6782)
                     /gene="eno"
                     /locus_tag="PK849_04650"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201525.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPE65447.1"
                     /translation="MAEDDWAVWSVMTKRLGSKIQIVGDDLFVTNPEKLSRGIEEGVA
                     NAVLIKLNQIGSLSETLNVIALAKRYGYATVISHRSGETDDSFISDLAVATGAGQIKT
                     GAPARIDRVAKYNQLLRIAEELDRAPYARLSQFRGNPNR"
BASE COUNT         1375 a         2072 c         1932 g         1403 t
ORIGIN      
        1 tgttcttcag cacagggctc agcgcgttga cgagtccgcc cgaagccgga cgcccggtca
       61 gttgtccgtc ctccgtcgag agagcgacgg gaaggcggtt tgaaaccacc agcagacgtt
      121 ctttcttttt catcgtttta tttcccttct tccctttgta tcttcatgag cgcgtccctc
      181 cagagcgcta cgtgaagcgc acgccgccgt cctttcgttc acacgtccct gccggtcaga
      241 tccatcgctc ccactcgcca tccggcgaat tcagggagac gaggagcgac aggtagcgcc
      301 gcaactgccc ggtaatgagg aagttctcga ggacgtactg ccgtgcgttc actcccatgc
      361 gctcccgctt ccacttgtgt gtgagaagat accggatacg aatggccgcg ccttcggggg
      421 agcgcacgcg gaacccgttg aaacggttga tgatctgcag gcgtattccg ccgacgtccc
      481 ctccgataac cggctttttc ttccacaagc cttcggtcac tgtaaggccg aagccctcct
      541 tggtcgattt ctgcatcact acgtcggcgc cggactgcag ggcgttgatc gtcctgtgcg
      601 catcggctgg aagaagcaag acgtgaatgt ccgggtcgcc cgccgccgcc tcctgaaccc
      661 tggcgagcac ctcgcccgat tcggggtcgt cggtcgcccc gcccccggcg agcacaaggc
      721 gaatatccac gtgctcgcgg gcaattctga agctttcaat aaccccgagg gggtccttga
      781 agcggtcgaa ccgcgagacc tgaaggatga ccggtttatc catagggata ccgagttcct
      841 cgtaggtctt cgcaacctcg cttttcggca gcgcgatatt cttttcgctc agggggtcga
      901 tgctcggcgt gatcagaaac tgggggtgcg ggagcgtctg ggcgaaatcg ggaagcgaga
      961 agatcgacgc gtcgtacccc tccacgtgct tcttgacaaa ccgccagacc gagcggttcg
     1021 gcgagctgac gtcgatatgg cagcgccaaa tccactttcc ctttctcttc ggaaaatacc
     1081 cgagcagcgg cgcgggctgc ggatcgtgga taaagacata atctgcatcc tcaaccacgc
     1141 cccgcagttt ctccgcattc gtgcgatttg tctcgacgta cgcttcgatc tgctgttccg
     1201 gcagcccccc cggcatcccc tgcaaagagt tatgaagggt ttttgtacag gcgaaaaagt
     1261 cgggattccc ttcgatgacc tcccaggaga cgtcgagtcc gagctccttc gagagcggga
     1321 ccatcgagga gagaatctcc gccacgcctc ccccctcttt cgtcgagttc acgtggacga
     1381 ttttcttccc ccgcagcata tccgcgatga ccatcaggct gtccatcgca tggaagccga
     1441 caacctcgcc gtattttttc agcatctcgg acatcgttat ttccccctcc tgtgcagatt
     1501 cttttcaaga agggaaacaa cctgcccctt caactcaccg agtgagaaga gatacgaatc
     1561 gatcgccctg agcgacttcc gaagcggctc cgtctcttcg ccgaactgct cgagccagag
     1621 agaaaagtcg tccttcccct cgggggagcg tctcctgccg tcgatgaagt gatgaaagaa
     1681 gctctgacgg ctcgccgtct tcaccgcaag cgggaagtcc ttcagcgagg agacctcgcg
     1741 ccccgtgtcg aacatcatct tggtgcaggc gataaagtag aattccctgt cgctcttctt
     1801 ccagctcata aaatcgccgt tctcaagacg cgcgtccaga atgtcctcaa ggatcgagcg
     1861 tatctcgcca agatcggcgc acgctacggg gttgatcgcg ctgagcgcct cggcgagctc
     1921 tatatctcca aggccgctgt cggcccacga ggcgaagtcg ttgttgaact cggattcggc
     1981 gatatggggg cgcagcatcc tcccccagaa gtggtagtaa atggaactga gcggcgtttg
     2041 cggaagcgcg gttcgaagct ctcgtatgga ttgcgcgcgc actccggtac tcagcgtgag
     2101 cagagcgcac tccatgaatc tgaacggaag tgttgttccg tgttctttca tttctgtttc
     2161 ctccttttcc cggccgatct catctccttt tatgttgtgg ccatgacatt attgtaagag
     2221 tataacacat aaacgccttg ttctacaaat tatcatgccc aaaaaagcgc tcgcctctgt
     2281 tctggcgagc gcttcggaat gacaatactc caaaaagcag gacagcggct acgccttctg
     2341 aggcgccttt atccaccatt cggcgagcac gccggaggcg atgtaaatgg agctgtacgt
     2401 gccgaccgtt atgccgatga gcagagcgaa cgagaagctg cgcagcacag ggcctcccca
     2461 gaggaagagc gcgatgacag gcagcagggt cgtcatggac gtgttgatcg ttctggagat
     2521 cgtttcattg atcgaattgt tcagcagggc gagcatcccg tttccgcgca gcgttttcca
     2581 gttctcgcgg accctgtcga ggacgacgat cgtgtcgttc agggagtagc cgacgatcgt
     2641 gaggatcgcc gcgataaacg acgaggagat ctccatgccg gtaagactga agacgccaag
     2701 cgtgatgatc gcgtcgtgca tcagcgtgag gacgcttacc acggcaaaac ggaactggaa
     2761 gcggaccgtg atatacgcaa ggataccggc aagcgcgatg aagagcgcca cgaaggcctc
     2821 tttccgcagt tcggcgccga ccacgggtcc gaccttttca agccggacgg ttttcatatc
     2881 gggatacttt gcccgaaggg cgtcgacaac catttttctg gcttcgtcct cgttggcgct
     2941 gatccggatg atgaagccga tatcgctgta ggactggata acggcgttcc cctggcccac
     3001 cgaagccagg acgtcgcgga catcgcccac ggagacgccc tgcgcgaact cgacctgcac
     3061 cacgttacct cccgtgaaat cgattcccag gttcagtccc ttcgtgaaga ccagaccgat
     3121 gctgacgacg acgcagacaa ggctgaccag gagcgcagcc ctgcgatgtt tcataaagtc
     3181 aatgaacatg attttcatag tcttttctcc acctctctta ggaacggtgc gccagggagc
     3241 tcgtcgcccg tccgcccatg aagtactgga ggagagagcg cgtgacgagg acgttgcaga
     3301 agacgctcga cacaataccg atgctgagcg ttaccgcaaa acccctgaca gggccgctgc
     3361 cgaagtagaa gaggaccgcc gccgcgatga gcgtcgtgat attcgcgtcg aagatcgtca
     3421 cgagggcctt tttgaatccg gcgtcgatgg ccgcgagcgg cgtcttgccg gcgcgcagct
     3481 cctccttcat gcgttcatag atgagcacgt tgccgtccac cgccatgcct atggtaagca
     3541 cgatacccgc gatgccgggc agggtcagcg tcgccttcag cgagatcagt cccgcgaaga
     3601 cgaggagaat ggccactgca agggccgcgt ccgcggctac cccgagaagg cggtagtaca
     3661 gcagcatgaa gagaaagaca agagacgctc caataagacc ggcgcgaata ccggagttga
     3721 tggagtcctc tccgaggctc ggtcccacgg aacggttttc gatgacgtca accgctacag
     3781 gaagcgcacc cgcgcggagc atgatggaga ggcggctggc ctcggctgcg gtgaaacggc
     3841 ccgagatctg ggcgttgccg ccggtgatgc gctcctggac tacgggagcg gagatgacga
     3901 cgccgtcgag gacaatggcg atctgccgtc cgatgttctc ggccgtcgca gcgtcaaaga
     3961 gcttcgcgcc ttcggagctg aacgatatgg cgacgactgc gcgtccgagg ttgtcgtact
     4021 gcgtcctggc gtccacgaga tcttttccgc ctaccaaaac tttcccgaga agataaaacc
     4081 tgccctcgtc tgcttttgct accacgccgc cctcaagaga agcggcctgc tgttcaagtt
     4141 ctcccttttg ggcctcaatc ttcgctacag cctcgttcca acgcgtctgc gcccgctcga
     4201 actcggcgtc gctgtcgtag ttcttccgct ctgcggaggg aggaaccggc gacgtcgccg
     4261 tcacaacctg gcggaactca agaagcgccg tcttgccgat aagctccaat gccgcgtccg
     4321 gatcctggat accggggagg tcgacgatga tcctgtcggt accctccctc tggatgacgg
     4381 gttccgccac accgtactgg tccacgcgat tccgaagcac agcaagaaga cgttcgatgc
     4441 tgtcctccgt caccttgctg tccgcggtgt ccttcgcctg aagaacgatg tgggcgccgc
     4501 ccttcaaatc tagccccaga ttgatccttc cctgaatggg aaagaccgag aacagggccg
     4561 caagcaccac gatggcgact attccaagcc gccagcggtc tcgtctcaac ataagaattc
     4621 cctccgttgt gttcgcaaaa ttggttgttc attcccccgc gaaaaaactc ccgccaacag
     4681 tatggccgac gggacgcgag cggggggatc gcctcgcggc gacctccccc cgtttccccg
     4741 ccgcacctgc ggttgcatcg gaagggggcg gggcgcactt cacatcgaac cgagagatac
     4801 gatcagctct tctcctcttc cttctcgcgt gcgggctcct ccttgggagc ctgggtggcg
     4861 ggagcgcgct tcgaagccac cgccgacttg agaaggcgta ccttcacgcc gtcggctatt
     4921 tcgacgacaa ggctgtcgtc gcgaacttca cggatggttc cgaagaaacc gccgatggtg
     4981 accacctggt cccccctcga aagcgatgag agaagttcgt cctgggcacg ctgccgcttc
     5041 ttctggggac ggatgatgaa gaagtagaaa atgaccacga atatcgcgag agggaagagc
     5101 attcccatga gacttccctg tcctcctgcg ccgcctgccg cctgtccaca ctgctgttcc
     5161 aaaaaaaacg cctccttgat attagggtgc aaggttgcct cgccctgccc ggcaagacgg
     5221 aaaacaaccc cgatattcta tcaaaaaaaa ctgctaacgt tcgcgatgct tcaggtaaaa
     5281 aagaagtttc tccagttcta tcgaaatatc cacgctgtag agcagcacgc tgtccggaac
     5341 ggagagcgtg accggggaga agttgagtat ccccttcacc gttcctgcag aaacgaccct
     5401 gtctgcaacg gactgggccg ctcccgtcgg cacggtgatg atgacgacat cgataccttc
     5461 ctctcgcagc acgcggggag cgtcctctat ggagtagcag ggagttccct ggatatgcgt
     5521 tccgattttc gaagggtcga cgtcgaagag ggccttaatc tgaaacttct cgttccggaa
     5581 cgcctgatac cctaaaagcg cctcgccgag ttttcctacg ccggccagac cgatggacca
     5641 gagcctgggg gacgcaagga ttccgccgat atgcttgaat aaacgatcaa catgataccc
     5701 cacgccccgc ttgcctatct cgccgaagta cgagaggtcc ttccgcacct ggctggcctt
     5761 gaacgagagc atctccccca tctccctgga ggagaccact ttctgcccct cggccgcgag
     5821 ctgctctaaa agacggtgat actggacaag ccgctcgact gtgggctccg ctatcttcgt
     5881 atccttcatg tgcgcacatc cttcaagagt agagggccgg aggcggcgcc tccggccgcc
     5941 ggaatcattc cttccaggcg acgacttcga tttccacaag ggctcctttt gggagcgcgc
     6001 tcacttcgac gaaggaacga gcggggggct ctttctcgaa atatgtcgcg tagacgccgt
     6061 tcaccgcagc aaagtcgttc atgctggtcg cgaagacggt cgccttgatc acatcggcaa
     6121 gggagtaccc ggcagcttca agaacggctt ttacgttgtt cagaacctgt tttgcctgct
     6181 cagcgacgcc gcccggaacg aactgccccg tcgccggatc gatgggaatc tgcccggagc
     6241 agaagaggaa ctgccccgcc ttgactgcct ggctgtacgg cccgaccgct gcgggcgcgt
     6301 tggttgtgga tacaatacct ttcaaaagga acgcctcctt aatgtctttt gtgtcctacc
     6361 tgttcggatt acccctgaac tgcgagagac gcgcgtacgg cgcccggtcc agctcctcgg
     6421 cgatgcgaag aagctggttg tacttcgcga ccctgtcgat gcgcgcgggg gctcccgtct
     6481 tgatctgccc ggctccggtg gccacggcga ggtcgctgat gaaggagtcg tcggtctcgc
     6541 cggaacggtg cgagatgacg gtcgcgtatc cgtagcgctt cgcgagcgcg atgacgttga
     6601 gcgtctcgga gagcgagccg atctggttga gcttgatgag aacggcgttg gccactccct
     6661 cctcaatacc tctcgacagc ttctccgggt tggtgacgaa gaggtcgtcg ccgacgatct
     6721 ggatcttgga gccgaggcgc ttcgtcatga cagaccatac cgcccagtcg tcctccgcca
     6781 tg
//
LOCUS       DAOPKU010000106         6747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12005_length_6747_cov_4.645696, whole genome shotgun sequence.
ACCESSION   DAOPKU010000106 DAOPKU010000000
VERSION     DAOPKU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12005_length_6747_cov_4.645696"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /gene="ptsP"
                     /locus_tag="PK849_04655"
     CDS             <1..1264
                     /gene="ptsP"
                     /locus_tag="PK849_04655"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPE65448.1"
                     /translation="ILVTRDFTASDMSQIQREMTLGVASEVGGRTSHAAIIARTMQIP
                     SVMGAHGLLSTVGNGDLLILDGETGEIFVNPDEETLARYKEKQERYECFLGRLSEIRG
                     KETVTLDGIKVELGANVGSSRDLDEVLKNDGEAVGLYRTEFLYLESPMLPTEDEQFEA
                     YKSVVEGMDGRPVVIRTLDIGGDKKLDYLSIPEEENPFLGYRAIRLCLDRQEIFKVQL
                     RAILRSSAYGNVRILFPMISCLEELREAKVILENTKDDLRQRKIPFDEGVLAGIMVEV
                     PSTAVLSDHFAKESDFFSIGTNDLIQYTVAVDRGNDKLAPLYSSYHPAVLRLVKTVID
                     NGKQAGIHVGMCGESAGDPKLIPILIGMGLTEFSMSPPSILHARWILRNLRKSDLEEA
                     ARHALSLGTAAEVEAYCGTLLQSLDLCR"
     gene            1225..1620
                     /locus_tag="PK849_04660"
     CDS             1225..1620
                     /locus_tag="PK849_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65449.1"
                     /translation="MRHAASVARPLPLNGGPAARGPIAVMSASIRGTNHSGLPEFLRP
                     LFWDAEWSAVDFRRNRTAIIERVLNLGDEAQLAWLKQNVAPEEIRAVVLSSRRLSKKT
                     ARCWQNYFGLREEEMRCFGMFSTNPDNLF"
     gene            1578..2216
                     /locus_tag="PK849_04665"
     CDS             1578..2216
                     /locus_tag="PK849_04665"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPE65450.1"
                     /translation="MFWDVLDKPRQSLLKALCANPPVHGVYLAGGTALALMFGHRQSE
                     DFDWFLPDSFNPGRLPGLLQPFGEVSVAETHAGTFHGWIDGIRVTWLHYPNPLLEPLQ
                     EPPDLPGLFMSSPRDTGLMKWAALADRGARKDFLDLYELSLQGVPLEDLFYLLPRKFP
                     GSRLNLYHMVKSLSFFDDAEGDPWPVMLRKVEWSVLKRHFLNVQRKLLGILG"
     gene            complement(2291..4300)
                     /locus_tag="PK849_04670"
     CDS             complement(2291..4300)
                     /locus_tag="PK849_04670"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPE65451.1"
                     /translation="MKRLGALIAVFILFSSLFATIGSAAEPAGYAATIAAAREIVWKT
                     LTTGGGSSATVAVMDGGKIVYSEQFGAAERATNRPADRETRFNIGSTSKMFGATAILL
                     LADEGKLSLDDPVAKHLPEFVMRDGRYREITVRMLFNHSSGLPGSSFIFEYEPAYDPH
                     AMLLDVLRESDLKHAPGAMGIYCNDGFTLAEMVVEKLSGKKFIDFVAERIFEPLGMKD
                     SAASVGETGGNVAVYYDVPTGKKYPLEVVRVHAAGGLSSTAEDLCRFADSFTPEGKHI
                     LSEASLAEALKSQPTLFTKHLHDAAMMDAFGWDYAWIPDYREKGVQVLAKSGGTMFYS
                     TNFQVVPAERLVVAVSIAGRADAPGVSRAILDALMKEKGLPFPGKPPLKKPGEPQPIP
                     SELLAFEGAYSDGERAVRFLFDPEKRTLNIHHLLPAPPQGEEAPPPVSLVYSDGVFHN
                     YEKGTSYYFASEGKNVYLIMEKIPGFGMNVPAFQRLEEIKEPKSLSIEMNGVLWMARN
                     ILPGAQIMNGTLMGWSSTSHDLPGYVFGFSLQKVEDFSFASIASTAFRDQFALQLSRK
                     DGKIRARTGIFVLSPADEAETLADGRNSVTIGADAENEWRKAGKDMILSFVRPEKGRV
                     IAVDPENDAALLYDSVVDSGEFFAPEGTFLFLAGAAGDVFEIEAR"
     gene            complement(4320..>6747)
                     /locus_tag="PK849_04675"
     CDS             complement(4320..>6747)
                     /locus_tag="PK849_04675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE65452.1"
                     /translation="GSASDLYLPDYSRLNEQLLSVLPVLPRGSSISILGRREDGALVY
                     LMDVRAAASALPGDEFRVRSSDFQYPFDERRPLLLGPFAHDDGSFFAALVPLVEPVTR
                     RVIAVFLLETDARNWRREPYRAALVPWGATILLLMVLFLGYRFSPRLRDSRVFVYAEV
                     LWVASMSLLLAGSCAWLVGAYEFRGRTRAFSMLSDFGSSALIQSLQELRDCSIEGLGR
                     FFESSQLVDAEEFAGFARHLIAIPHVDEVLWAPAVPSLAAESGDLSEAGLLSSGDLTE
                     GPSMPSSSSYALSFPVLYSVSADRRILPPGTDLRSIPSLKHAIELSVRNGMKTASEAA
                     TSDVGSDGACRSVLFRPVRSAGGDLMGVVGVVLNLEVLLNDTMLRDEGAQQHVMDVEL
                     KRLLPGGEERLLLSMPSGADALPRERNAESFHSLRPFFVFGRAFVLETRSGEGFASMH
                     PERMGKIVLFFGIAVSALITLLAGLFLNWRRTLLRFVTIKTVSLAQSEFRSRKLAHLY
                     RSITEGVVITDAEGLIEECNDALEELTGFALEEMQGRSLAIFSAREGQYDHEFLRSLG
                     QNGVWQGEVWNRKRDGEVYPVWLTIAAVQDDSGQTTHYSGVMRHIGGIKSEQERLNRM
                     AYYDFLTGLPNRALFTDRLEVAMARAKREGSMVALVFLDLDRFKEVNDAFGHEAGDAL
                     LIAVADRLSGAFREQDTVARLAGDEFVLLFEGLRSDGELDPLHERLEALFQEPFTADG
                     RSISMEASFGVAFFPEDGADVRSLLDIADRKMYAEKAARRKRRGAASGSDEEENGRSG
                     TFDGNAEEEG"
BASE COUNT         1328 a         2151 c         2036 g         1232 t
ORIGIN      
        1 catcctcgtc acccgcgact tcaccgcgtc cgacatgtcg cagatccagc gcgagatgac
       61 gctcggcgtc gcctcagaag tcggaggcag gacctctcat gcggcgatta tcgcccgcac
      121 gatgcaaatc ccctccgtga tgggggcgca cgggctgctg agcacggtag gcaacggcga
      181 cctgctcatc ctcgacggcg agacggggga gatcttcgtg aaccccgacg aggagacgct
      241 ggcgcgctac aaggaaaagc aggaacggta cgaatgcttc ctcggacgcc tctccgaaat
      301 ccgcgggaaa gagaccgtca cgctcgacgg catcaaagtc gagctgggcg cgaacgtcgg
      361 ttcctcccgc gatctcgacg aggtgctgaa gaacgacggc gaggccgtcg gtctctatcg
      421 aacggagttc ctctacctcg aaagcccgat gctccccacc gaggacgagc agttcgaagc
      481 ctacaagagc gtcgtcgagg ggatggacgg ccgtccggtg gtcatcagga cgctcgacat
      541 cggcggcgac aagaagctcg actacctctc cattccggag gaggagaacc cgttcctcgg
      601 ctaccgcgcc atccgtctct gcctggaccg gcaggagata ttcaaggtgc agctccgggc
      661 gatcctccga tcgagcgcct acggcaacgt gcgcatcctc ttcccgatga tctcctgtct
      721 tgaggagctc cgggaagcga aggtcatcct cgaaaacacg aaggacgatc tccgtcagcg
      781 gaagattccg ttcgacgagg gcgtcctggc gggcattatg gtggaggttc cctcgaccgc
      841 cgtgctctcc gaccacttcg cgaaggagtc cgacttcttc agcatcggca ccaacgacct
      901 catccagtac accgtggcgg tcgaccgcgg aaacgacaag ctggctcccc tctactcatc
      961 ctaccaccct gcggtgctcc ggctcgtgaa gaccgtgatc gacaacggca agcaggccgg
     1021 tatccacgtc gggatgtgcg gcgagtcggc gggagatccc aagctgattc cgattctgat
     1081 cggaatgggg ctgacggagt tcagcatgag ccctccgtcc attctgcacg cccggtggat
     1141 tctgcggaac cttcggaaga gcgacctgga agaagcggcg cggcacgctc tttcgctggg
     1201 gacggctgcg gaggtggagg cgtattgcgg cacgctgctt cagtcgctcg acctctgccg
     1261 ctgaacggag gaccggccgc gcgcggcccg atcgccgtca tgagcgcatc aatccgcggg
     1321 acgaatcatt ccggactccc cgagtttctg cgtcccctct tctgggacgc ggaatggtcc
     1381 gctgtggact ttcggcgcaa ccgcacggcg atcatcgaac gggtgctgaa cctgggggac
     1441 gaggcgcagc ttgcgtggct gaaacagaat gtcgcgccag aagagatacg cgccgtggtg
     1501 ctttcgagcc gccgtctgag caaaaagacc gcccgctgct ggcagaacta cttcggtctg
     1561 agggaggagg aaatgcgatg ttttgggatg ttctcgacaa accccgacaa tctcttctga
     1621 aggctctctg cgcgaatccc cccgtccacg gcgtctatct cgccggaggc accgcgctcg
     1681 cccttatgtt cggccatcgc cagtcggagg acttcgactg gtttctcccc gacagtttca
     1741 atccgggacg ccttcccggc cttctgcaac cgttcggcga ggtctccgtg gcggagacgc
     1801 acgccgggac gttccacggc tggatcgacg gtatccgcgt tacctggctt cactacccca
     1861 accccctcct tgaaccgctg caggagccgc cggatcttcc ggggcttttc atgtcctccc
     1921 cgcgcgacac cggtctgatg aagtgggccg ctctggccga ccggggagcg cgcaaagatt
     1981 ttctcgacct ctacgagctc tcgttgcagg gcgtcccgct cgaagacctc ttctatctgc
     2041 tcccgcgcaa gtttccgggc agccgcctca acctctacca catggtgaaa agcctcagct
     2101 tcttcgacga cgccgagggc gacccgtggc cggtgatgct gcggaaggtc gagtggagcg
     2161 tgctgaagcg tcacttcctg aacgttcaga ggaagctgct gggaattctg ggataacgaa
     2221 aagaggaggg gaagcgcagg ttgtcgcgcc ccctcctctt tgtctgtccg gctgtaaaaa
     2281 atggacggct ctatctggcc tctatctcga acacatcccc cgccgcgccc gcgaggaaga
     2341 gaaaggttcc ctccggcgcg aaaaactccc cgctgtccac aacgctgtcg taaagcagcg
     2401 ccgcgtcgtt ttccgggtcg accgcgatca cccgtccctt ttcgggccgg acgaaggaga
     2461 ggatcatgtc cttgccggcc ttccgccact cgttttcggc gtcggcccct atcgtcacgc
     2521 tgtttcttcc gtcggcgagc gtttccgcct cgtcagccgg agagagaacg aatattccgg
     2581 tcctcgccct gattttgccg tccttccggg agagctgaag ggcgaactgg tcgcggaaag
     2641 ccgttgacgc tatgctcgcg aagctgaaat cctcgacctt ctgcagagag aaaccgaaga
     2701 cgtacccggg cagatcgtgg ctcgtggacg accagcccat cagcgtcccg ttcataatct
     2761 gcgccccggg aagtatgttc cgcgccatcc agagcacccc gttcatctcg atggaaagag
     2821 atttcggctc cttgatctcc tccagcctct ggaacgccgg gacgttcatg ccgaagccgg
     2881 gaatcttctc catgatcagg tagacgtttt ttccctcgga cgcgaagtag taggaggttc
     2941 ccttctcata gttatgaaac actccgtcgc tgtagaccaa agagacggga ggaggcgctt
     3001 cctctccctg cggaggcgcg ggaagcaggt ggtggatatt cagcgtccgc ttctccgggt
     3061 cgaagaggaa acggaccgcc ctctcgccgt cggagtacgc tccctcgaag gccaagagct
     3121 cggaggggat aggctgcggc tccccgggtt ttttcagagg cggcttcccg gggaagggga
     3181 gccccttctc cttcatgagc gcgtcgagta tcgcacggct aacgccggga gcgtcggctc
     3241 tgcccgcgat actcacagcg acaaccaggc gctcggcagg aacaacctgg aagttggtgg
     3301 agtagaacat cgtcccgccg ctctttgcga gcacctgcac acccttctcg cgatagtccg
     3361 gaatccacgc gtagtcccac ccgaaggcgt ccatcattgc ggcgtcgtgc agatgtttcg
     3421 tgaaaagggt cggctgactc ttcagtgctt ctgcgaggga ggcctcgctc aggatatgct
     3481 ttccttcggg cgtgaagctg tccgcaaaac ggcacagatc ctcggcggtg gaggagagtc
     3541 ctcccgccgc atgcacgcgg acgacctcaa gagggtattt tttgccggtc gggacgtcgt
     3601 agtacacggc gacgttgccg ccggtctcgc cgacgctcgc ggcgctgtcc ttcattccca
     3661 gcggctcgaa gatgcgctcc gcgacgaagt cgatgaactt cttccccgag agcttctcca
     3721 cgaccatctc cgcgagggtg aagccgtcgt tgcagtagat acccatggcc cccggcgcgt
     3781 gcttcagatc cgactcgcgc aggacgtcga gcaacatcgc gtgcggatcg tacgcgggct
     3841 cgtactcgaa aataaagctg gaaccgggaa ggcccgagga gtggttgaag agcatccgca
     3901 ccgtgatctc cctgtaccgg ccgtcgcgca taacgaactc gggcaggtgc ttcgccacgg
     3961 gatcgtcgag ggagagtttc ccctcgtcgg ccagcagcag gatcgccgtc gcgccgaaca
     4021 tcttgctggt ggagccgatg ttgaagcgcg tctctctgtc cgcggggcgg ttcgtcgcgc
     4081 gctccgccgc gccgaactgc tcggagtaga cgatcttccc tccatccatg acggccacgg
     4141 tcgcggaact tcctcctccg gtcgtgagcg tcttccagac gatctccctg gcggcggcga
     4201 tggtcgccgc gtatccggct ggttcggccg ccgagccgat agtcgcgaac aacgaggaga
     4261 acaggatgaa aactgcaata agagcgccaa gccttttcac gattctttca cccttccctt
     4321 tacccttctt cttcggcgtt tccgtcgaaa gtccctgaac ggccgttttc ttcttcgtcg
     4381 gatccggaag ccgctcccct gcgctttctg cgcgctgctt tttcagcgta catctttctg
     4441 tcagcaatgt ccagaagaga acggacatct gctccgtcct ccgggaaaaa cgcaacgccg
     4501 aagctcgcct ccatcgaaat ggaacgccca tccgcagtaa acggctcttg aaaaagcgcc
     4561 tccaggcgct catgcagcgg atcgagttca ccatcggaac gcaacccctc gaagaggagc
     4621 acaaattcgt cccccgcaag gcgtgcaacc gtgtcctgtt cgcggaacgc gccgctcaaa
     4681 cgatccgcca ccgcgataag cagagcgtcc ccggcttcgt gcccgaaggc gtcgttgacc
     4741 tccttgaagc ggtcgagatc aagaaagacc agcgcgacca tcgaaccctc gcgtttcgcc
     4801 ctcgccatgg cgacctccag gcggtcggtg aagagcgcgc ggttgggcag cccggtcagg
     4861 aaatcgtagt atgccatcct gttcaggcgc tcctgctcgc tcttgatgcc gccgatgtgc
     4921 cgcatcacgc cggagtagtg cgtcgtctgc cccgagtcgt cctgaaccgc cgcgatggtg
     4981 agccagacag ggtacacttc tccgtccctc ttccggttcc acacctcgcc ttgccatacc
     5041 ccgttctgcc cgagcgagcg caggaactca tgatcgtact gcccctccct ggcggagaag
     5101 atggcaagcg atctcccctg catctcctca agcgcgaagc ccgtcagctc ttcgagcgcg
     5161 tcgttgcact cctcgatgag gccctccgca tccgttatca ccactccctc cgtgatggag
     5221 cggtagaggt gcgcgagctt gcgggaacga aattcgctct gcgccagaga gaccgtcttg
     5281 atcgtcacga agcggagcag cgtccgccgc cagttgagaa agagcccggc gagcagcgta
     5341 ataagcgccg agacggcgat accgaagaag agaacgatct tccccattcg ttccgggtgc
     5401 atcgaggcga atccttcccc ggagcgcgtc tccagcacaa aagcccgacc gaacacgaag
     5461 aaggggcgca gagagtgaaa agactccgcg ttccgttccc ttggaagcgc atccgctcct
     5521 gatggcatgg agagcaacag gcgctcttcc ccgccgggca ggaggcgctt gagttcaaca
     5581 tccatgacgt gctgttgcgc cccctcgtca cgcagcatcg tatcgttcag caaaacctcc
     5641 aggttgagca cgacgccgac aacgcccatc aggtcgcccc cggcgctccg cacaggacgg
     5701 aaaaggacgg aacggcatgc cccgtcggaa ccgacgtcgc tcgtcgccgc ttcggaggcg
     5761 gtcttcatgc cgtttcgtac agagagctca atggcatgtt ttaaagaggg aatggatcgc
     5821 agatcggtcc ccggaggcag tatcctccgg tcggcggata ccgaatagag caccgggaag
     5881 gagagcgcgt aggaagatga agaaggcatg gaaggtcctt ccgtcaaatc cccggaagag
     5941 agaagtccgg cttcggacag gtcgccgctt tcggctgcta aagagggcac ggccggcgcc
     6001 cacagcactt cgtcaacatg gggtatggcg atcagatgtc tggcgaagcc cgcaaattct
     6061 tcggcgtcga caagctggct gctctcgaaa aaacgtccca atccttcgat gctgcaatcc
     6121 cgcagctcct ggagcgactg tatcaaggcg ctggagccga agtcggagag catggagaac
     6181 gccctcgtac gcccgcggaa ttcgtacgcg cccacgagcc atgcgcacga tccggcgagg
     6241 agaagactca tcgaggcaac ccagagcacc tccgcgtaca cgaaaaccct cgagtcgcgg
     6301 agtcgtgggg agaagcggta gcctaaaaaa agaaccatga ggaggagtat cgtcgctccc
     6361 cacggaacaa gcgccgccct gtacggctcg cgccgccagt tccgggcgtc ggtttcaaga
     6421 aggaatacgg cgatgacacg gcgcgtcacc ggttccacca aagggaccag cgccgcaaaa
     6481 aaagagccgt cgtcgtgagc gaaaggaccg agcagaagcg gccgtctttc gtcgaaaggg
     6541 tattggaagt cggaggaccg gacacggaat tcatcgccgg ggagcgcgga tgcggcggcg
     6601 cgcacgtcca tcaggtagac gagggcgccg tcctccctcc tcccgagaat agatatggaa
     6661 gagccgcggg gcagcacggg aagaaccgag aggagctgct cgttcaggcg ggagtagtcc
     6721 ggaaggtaca ggtcggaagc gctgccg
//
LOCUS       DAOPKU010000107         6718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12079_length_6718_cov_5.487618, whole genome shotgun sequence.
ACCESSION   DAOPKU010000107 DAOPKU010000000
VERSION     DAOPKU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12079_length_6718_cov_5.487618"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PK849_04680"
     CDS             <1..400
                     /locus_tag="PK849_04680"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HPE65453.1"
                     /translation="EKSGSRTNLLGVTVLTSFDASWAEVAPGCSLSDALEKRAALCRE
                     TGIDGIVCSPLDLPVVRVAGAGLFTVVPGVRPASADDDQARVATPQKTIANGADYIVV
                     GRPILNAPDRIAAVRSIAASIEEGLKCRSL"
     gene            385..975
                     /gene="pyrE"
                     /locus_tag="PK849_04685"
     CDS             385..975
                     /gene="pyrE"
                     /locus_tag="PK849_04685"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPE65454.1"
                     /translation="MQESVMLAKLQEMMKESGAHLEGHFLLTSGNHSSDYMQCALLLR
                     FPRYAAFAGEALAEKVRRYSPDLIASPALGGLIIGHEVARALDVPFIFCERREGEMVL
                     SRFPFPEGKRVVVIEDVVTTGVSSGEVGSVLERGGATWVGTGAVIDRSGGKSSLPHVP
                     ESLWKVAFPLWKPEECPLCAKGIPLVKPGSRTKTAP"
     gene            1218..1616
                     /locus_tag="PK849_04690"
     CDS             1218..1616
                     /locus_tag="PK849_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65455.1"
                     /translation="MKNTTRLLLVSGLLCLLVFLAYLFLFSGQEAPEPLDGDPLRERG
                     MRAAAIGGELVSVAKEQLGALQEEHRADVGELLGELERKTAAVKELLSRKDADEEDLA
                     AAVSALEKALDGVLVVPDNETGNEERRTDS"
     gene            1613..2113
                     /locus_tag="PK849_04695"
     CDS             1613..2113
                     /locus_tag="PK849_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65456.1"
                     /translation="MTSVNETAVLARRVNEMLRRYMKVQEDLFKPSLRKILRIPGIYR
                     PIDYAENLRALEELLRELAEVKAAIRREEPDAASPEGKFLGVLRGYVSLMTSAVEKLE
                     NICSRLKERSEGGAYGKDEYKSDMAALREIQKKHLESGVALNEMMKTLSRSDPPKETQ
                     DDESKS"
     gene            2344..2997
                     /locus_tag="PK849_04700"
     CDS             2344..2997
                     /locus_tag="PK849_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE65457.1"
                     /translation="MREKKKDQAYETIRTLVLQRRSSDSFVISENSLAAELNMSRTPI
                     REALQRLQMEGFIEIHPHRGIVIPEVSVIEVNETFALRMAIEEFVIKEIIPFMTEQRT
                     AEMDRNLALQKEAVDKGDVSEYLRHDKEFHDHFLRIYSNALIFKTVQRVRDRFISMGA
                     NVLREPGSVERSFAEHCSIADAVRRSDRGGAAEAMHRHLITGRNNVLSMPGNLGAGI"
     gene            3159..4163
                     /locus_tag="PK849_04705"
     CDS             3159..4163
                     /locus_tag="PK849_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HPE65458.1"
                     /translation="MYAQPVRKEATLSPAKYEKDLLGYFYKTMVTIRSFEQMIEKFFL
                     EGEIPGFVHLYIGEEAIATGVMANLRKTDYIQSTHRGHGHTIAKGADLKRMMAELFGK
                     STGSCKGKGGSMHIADFSVGMLGANGIVGGGFTMATGAALAHKMQKTDGVAVTFFGDG
                     ASNRGTFHEAANMAAAWNLPVIFVCENNQWASTTPYRTTTSVDDIADRAVAYGMPGVI
                     VDGNDVFSVCEAAKELVERARRGEGPSILECKSYRIKGHFVGDPEKYRTKEEVQKVFG
                     ETDPIPRFESKVLKAKAMTKKELETIRNTVEEELKEALEFARTSPVPEESALFEGLYV
                     "
     gene            4177..5148
                     /locus_tag="PK849_04710"
     CDS             4177..5148
                     /locus_tag="PK849_04710"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HPE65459.1"
                     /translation="MRTISFSQATLEAMQEEMARDEKVFVMGEDIVRQGGIFGQFKGL
                     PEKFGTERVMDTPISETAIVGACVGAALAGMRPVADMHFADFAGVCFDEIYNQMAKIY
                     YMFGAQKPVSMVLRAPDGLVNQAAAQHSQSLEAFFMHIPGLKVVIPSNPADAKGLLKA
                     AIREDNPIIYFEHKALFSMKGEVPEEEYLTPIGKAKVVKEGSDVTLVSYSMTMNLCVK
                     AAELLAKDGISAELIDLRTISPLDKETILESVAKTTRLAIAHEAVKQGGVGAEIAALV
                     AEEAIDCLDAPIVRVGAPFTPVPFARPLEQAYRITPEKIAAAVRAMF"
     gene            5158..5649
                     /locus_tag="PK849_04715"
     CDS             5158..5649
                     /locus_tag="PK849_04715"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPE65460.1"
                     /translation="METERVSSAAAEIGRTILVRLAPGEDVFEGLRTVCERHGLRSGR
                     IATMIGSLRAASLVCVTPDPDAPGRVVYLEPRTIEGPLELIAVQGVFGWDDDKDELSI
                     HLHAQVAGEDLRPFAGHLADTGKNRILATAEIVLDVFEGAELRRSFDEETGFTLFKIF
                     PKL"
     gene            5785..>6718
                     /locus_tag="PK849_04720"
     CDS             5785..>6718
                     /locus_tag="PK849_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE65461.1"
                     /translation="MKKLVALLILVTALCAFSVPAFAAYKAEYKLDIVPSLTTSWGMG
                     AVYFTELVKERSGGKINIKVYPGSQLTVGAQTNAFMLVRNGTIDFAVQSSINYSPQII
                     ELNLFALPFFIANQPDKYAALDAVKAGKSGEMVVKAVEDKGVKFLAWGENGFRELTNS
                     KKEIRTPQDMEGMKIRVVGSPIFLDAFRAIGANPVTMNWSDTMAAIQQGVVDGQENPI
                     STFYPVKMHEYHKFVTDWHYVVDPTLYVVNPGVWKSFSAEDQKMIQEAAVEAGKYMIA
                     LARISLDDGTAHKYLESIGKLPEITDWYKELAKVG"
BASE COUNT         1455 a         1864 c         2118 g         1281 t
ORIGIN      
        1 agagaagtcg gggtcgcgga cgaacctgct cggcgtgacc gtcctcacga gcttcgacgc
       61 ctcgtgggcg gaggtggccc ccgggtgttc gctttccgac gcgctcgaaa aacgcgccgc
      121 gctctgccgg gagaccggaa tcgacggcat cgtctgctcg ccgctcgacc ttccggtggt
      181 ccgcgtagcc ggggccgggc tcttcaccgt ggtcccgggg gttcgccctg cgagcgccga
      241 cgacgaccag gcgcgcgtcg ccacgccgca gaaaacaatc gcgaacggcg cggactacat
      301 cgtcgtgggg cgtccgatac tgaacgctcc ggacagaatc gcggcggtga gaagcatcgc
      361 cgcgtcgata gaggaggggc tgaaatgcag gagtctgtaa tgctggcgaa gttgcaggag
      421 atgatgaagg agagcggcgc gcaccttgaa ggccactttc tgctgacttc ggggaaccac
      481 agcagcgact acatgcagtg cgccctgctg cttcgctttc cgcgctacgc cgccttcgcc
      541 ggagaggcgc ttgcggagaa ggtgcgccgc tactcgcccg accttatcgc ctcgcccgct
      601 ctcggcggcc tcatcatcgg gcacgaggtt gcgcgcgcgc tcgacgttcc cttcatcttc
      661 tgcgagcgaa gagagggcga gatggtcctc agccgcttcc cgttccctga ggggaagcgc
      721 gtcgtggtga tcgaggacgt ggtcactacc ggcgtctcct ccggcgaggt aggaagcgtt
      781 ctcgaacgcg gcggcgcgac atgggtcggg acgggggccg tcatcgacag gagcggcggg
      841 aagtcctcgc tccctcatgt tccggagtcg ctttggaagg tggcgtttcc gttgtggaag
      901 cctgaagagt gtcctctctg cgcgaagggt attccgctgg tgaagccggg aagcaggacg
      961 aagaccgctc cctgaaccac agcggaaggg gctgatttac tgtcgccccg aacttccggc
     1021 agtcagcatc ggagcttcct ctgacgcgat tcggatggtt tttcaagaaa cacgccccct
     1081 tgatgtgccg ggggcttttt tctttgccgt attcagtgct acaatacacg gtagtaagta
     1141 attttacgta ccggagccgt cgcagatttc agggtggccg gctgtgttgc gagtacgttg
     1201 ttctggagga agatcgcgtg aaaaacacaa ctcgtctgct cctggtgtca ggactgctct
     1261 gcctgctcgt ttttcttgcg tacctttttc tcttctccgg acaagaagcc ccggagcctc
     1321 tcgacggcga cccgttgagg gagcggggga tgcgcgcggc ggcgatcggc ggcgaactcg
     1381 tttcagtcgc caaagagcag ctcggcgcgc ttcaggaaga gcacagggcg gatgtagggg
     1441 agctgctcgg cgaactggag cggaagaccg ccgcagtgaa ggagctcctt tcccgaaagg
     1501 acgccgatga agaagacctg gccgcggcgg tgtccgcact cgaaaaagcg ctcgacggcg
     1561 tgctggtcgt ccccgacaac gaaacaggaa acgaggaaag gaggacggat tcatgacatc
     1621 cgtaaacgaa acagccgttc ttgcgcggcg cgttaacgag atgctccgaa ggtacatgaa
     1681 ggtgcaggag gatctattca aaccgtcttt gcgcaagata ctgcgtatac cggggatcta
     1741 caggccgatc gactacgcgg agaatcttcg cgcgctcgaa gagctgctgc gggagctcgc
     1801 cgaagtcaag gccgctatcc ggcgagaaga acccgatgcg gcgtctccgg aggggaagtt
     1861 tctgggggtg cttcggggat atgtctcctt gatgacctcg gctgtcgaaa agctggagaa
     1921 catctgctcc cgcctgaagg aacgaagcga ggggggggcg tacggcaaag atgaatacaa
     1981 gagcgacatg gcggcgctgc gcgaaattca gaaaaaacat cttgagtccg gcgtcgcgct
     2041 gaacgaaatg atgaagacgc tctcccgcag cgacccgccc aaagaaacgc aggacgacga
     2101 atcgaagagc tgaaaacaaa gcccccggag aaacgctccg agggtttttt cattgccttc
     2161 cttccctctg ccctgaaagc ggcgcatctg ttttccccct tcgtttctgc gggcactttt
     2221 tttaaacttt attttatcct tgagaccctc ttgacatttc cgttacacaa agggtatcct
     2281 atcaaatgta tgaagcttgt attttgcttg tatacaggaa agcgtttcga gggggagagc
     2341 gccgtgcggg agaagaagaa agaccaagcc tacgaaacaa ttcggacgct ggtactccag
     2401 cgcagatcct ccgattcctt cgtcatctcc gagaactccc tggccgccga gctgaacatg
     2461 agcagaacgc cgatacggga agcgctgcag cgtctccaga tggaggggtt catcgagatc
     2521 cacccgcacc gcggcatcgt cattcccgag gtctcggtaa tcgaagtgaa cgagacgttt
     2581 gctctccgga tggcgatcga ggagttcgtg atcaaagaga tcatcccctt tatgacggag
     2641 cagcggacgg cggagatgga ccgcaatctc gcgcttcaga aagaggccgt cgacaagggc
     2701 gacgtctctg agtatctccg ccacgacaaa gagtttcacg accatttcct ccgtatctat
     2761 tccaacgctc ttatcttcaa gacagtacag cgtgtaaggg accgtttcat ctccatgggg
     2821 gccaacgtcc tccgcgagcc ggggagtgtc gaacgatcct tcgcggagca ttgcagtatc
     2881 gccgacgccg ttcgcaggag cgacaggggc ggcgctgccg aggctatgca ccgtcatctg
     2941 attacaggca gaaacaatgt tctctctatg ccgggaaatc tcggcgcggg gatttgagag
     3001 ctctacgcgg ccgccccgtt cttcgtgacc gggagaggcc gcggcggatt cgaggaaggg
     3061 ggggcgcttt ttcgaagaac cgcgcatgaa caagaagaag agggccgcgt aaagggccgt
     3121 attcggaaaa agaccagact ttcaacgagg tgatttgtat gtatgcgcag cctgtgcgga
     3181 aagaggcgac tctttctccc gcaaaatatg aaaaagatct gttgggctat ttctacaaga
     3241 caatggtgac gatcaggagt ttcgagcaga tgatcgagaa gttctttctt gaaggcgaga
     3301 ttccgggatt cgtccacctg tatatcggcg aggaggcaat cgcgacaggc gtcatggcca
     3361 acttgcgaaa gaccgattat atccagagca cgcaccgcgg gcacggccac accatcgcca
     3421 agggagcgga tctcaaacgg atgatggcgg agctcttcgg caagagcacc ggaagctgca
     3481 agggaaaggg cggttcgatg cacatcgccg acttcagcgt ggggatgctc ggcgcgaacg
     3541 gcatcgtcgg cggcggattc acaatggcga caggcgccgc gttggcgcac aaaatgcaga
     3601 agaccgacgg cgtcgcggtc actttcttcg gcgacggcgc atccaaccgg ggaactttcc
     3661 acgaggcggc gaatatggcc gcggcgtgga atttgccggt gatcttcgtc tgcgagaaca
     3721 accagtgggc ttcaacgact ccgtatcgca ccacgacttc tgtggacgac atcgccgacc
     3781 gggccgtcgc ttatggaatg ccgggcgtta ttgtggacgg gaacgatgtt ttctctgtct
     3841 gcgaggcggc gaaggagctc gtcgaaagag cgcgccgggg ggaggggcct tctattctgg
     3901 agtgcaaatc gtaccgtatc aagggacact tcgtcggcga ccccgagaag taccgtacga
     3961 aggaagaggt ccagaaggtc ttcggcgaga ccgatcccat cccccgcttc gagagcaaag
     4021 tgctcaaggc gaaggcgatg acgaaaaaag aacttgaaac catcaggaat acggttgaag
     4081 aagaactgaa agaagccctg gagtttgccc gcacttctcc cgtaccggag gagtcggcgc
     4141 tctttgaagg gctctacgtg taaggcggtg gacaagatga gaacaatcag tttttcccag
     4201 gcgacgctgg aagcgatgca ggaggagatg gcccgggacg agaaggtctt cgtcatgggt
     4261 gaggatatcg tacgtcaggg cggcatattc ggtcagttca aagggctccc tgaaaagttc
     4321 ggtactgaac gggtgatgga tacccccatc agcgagacgg cgatagtggg cgcctgcgtg
     4381 ggcgcggcgc ttgccgggat gcgtccggtc gcggacatgc actttgcgga cttcgccgga
     4441 gtctgcttcg acgagatcta caaccagatg gcgaagatct actatatgtt cggcgcgcag
     4501 aagcccgtct ccatggtatt gcgcgcgccg gacggccttg tgaatcaggc tgcggcgcag
     4561 cactcgcagt ctcttgaggc gttcttcatg cacatcccgg gactgaaggt cgtcatcccc
     4621 tcgaacccgg cggatgcgaa ggggttgctg aaggcggcga tccgcgagga caacccgatc
     4681 atctacttcg agcacaaggc gctcttctcc atgaagggag aggttccgga ggaagagtac
     4741 ctgacaccca tcggcaaggc gaaggtcgta aaagagggaa gcgacgtgac gctcgtttcg
     4801 tactcgatga ccatgaatct ctgcgtcaaa gcggcggagt tgctggcgaa ggacggcatc
     4861 agcgccgagc tgatcgacct ccggacgatt tcgcctctgg ataaggagac gatcctcgag
     4921 tcggtggcga agaccacccg cctcgccatc gcgcatgaag ctgtcaagca gggcggcgtg
     4981 ggcgcggaga tcgcggctct cgtcgccgag gaggcgatcg actgccttga cgcccccatc
     5041 gtccgcgtcg gcgctccgtt caccccggtt cccttcgcgc gcccgctgga gcaggcgtac
     5101 cggattactc cggagaagat cgccgccgcc gtcagggcga tgttctagga agaggccatg
     5161 gagacggaac gggtttcgtc ggcggctgcg gaaattggac ggacgattct cgttcgtctc
     5221 gctccgggag aggacgtctt cgaggggctg cgcaccgtat gcgaacgcca tggactccgg
     5281 tccgggcgaa tcgcaacgat gatagggagt ctgcgcgccg cgagcctcgt ctgcgtgact
     5341 cctgatccgg acgctccggg gcgggtggtc tatctcgaac cccggactat cgagggcccg
     5401 cttgaactga tcgcagtaca gggagttttc ggctgggacg atgacaaaga tgaactttcg
     5461 atccatctgc acgcgcaggt ggccggggag gatcttcgtc cgttcgcggg gcatctggcc
     5521 gatacgggga agaaccggat tctcgccacg gcggagatag ttctcgacgt ttttgaaggc
     5581 gccgaactca ggcgctcgtt cgacgaggag acagggttca cgcttttcaa gatttttccg
     5641 aaactgtaat catacgttac aatacaagga caatgtcgct ggaacgagga ggtgatcgct
     5701 ccgctaccga aggaagtgtg gaaggaaagg cgctattccg aacatgggaa gttgtaaaac
     5761 cacattcagg aggtaaagaa gaagatgaaa aaactcgttg cgctgctcat acttgtaaca
     5821 gctctgtgcg cgttctccgt tccggccttt gcggcctaca aagccgagta caaactcgat
     5881 atcgtcccca gcctcaccac atcctggggt atgggcgccg tctatttcac cgaacttgtg
     5941 aaggagagaa gcggcggcaa gatcaacatc aaagtgtatc ccggctccca gcttaccgta
     6001 ggagcgcaga cgaacgcgtt catgctcgtc cggaacggga cgatcgactt cgcggtgcag
     6061 tcctccatca actactctcc gcagatcatc gagctgaacc tcttcgccct tccgttcttc
     6121 atcgcgaatc agcccgacaa gtacgccgcg cttgacgcgg tcaaggcggg gaagtccgga
     6181 gagatggtcg tcaaggccgt cgaggacaag ggtgtgaagt tcctcgcatg gggagagaac
     6241 ggcttccgcg aactgacgaa ttcgaagaaa gaaattcgca ctcctcagga tatggaagga
     6301 atgaagatcc gcgtggtcgg cagccccatc ttcctcgacg cgttccgggc gatcggcgcc
     6361 aaccccgtca cgatgaactg gagcgatacc atggcggcca ttcagcaggg cgtcgtggac
     6421 ggccaggaga accccatcag cacgttctac cccgtgaaga tgcatgagta ccacaagttt
     6481 gtcaccgatt ggcattatgt cgtcgacccc acgctctacg tcgtgaaccc cggcgtgtgg
     6541 aagtccttct cggcagagga tcagaagatg attcaggaag ccgccgttga ggcaggcaag
     6601 tacatgatcg cgcttgcccg tatcagcctg gacgacggga ccgcgcacaa gtacctggag
     6661 agcatcggca agctgcccga gatcaccgac tggtacaaag agctcgccaa ggtcggca
//
LOCUS       DAOPKU010000108         6698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12140_length_6698_cov_4.019871, whole genome shotgun sequence.
ACCESSION   DAOPKU010000108 DAOPKU010000000
VERSION     DAOPKU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12140_length_6698_cov_4.019871"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PK849_04725"
     CDS             complement(<1..927)
                     /locus_tag="PK849_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPE65462.1"
                     /translation="MNKQEFIRWIEEHRAIVPLDGGMGTLLAEKGWTPPMLPEEMNIH
                     SPEVVAGIHAAYINAGVRIIETNTFGGTPIKMAHRGLREKAREINARAARLAKSAAGE
                     RALVAGSVGPLGELLAPFGSLTFDEAYASFRTQMEGLLEGGADFLLIETMIDLREAKA
                     AVLAAKDVNPDGAFVVSFTFDQKGRTVTGTPPEVAARWAAYQGAAGVGANCGVGPEAY
                     ISTVEVLSEHAGLPVFVYANAGLPGNEEYRSPEVFATWGKRLAEAGATVIGGCCGTTP
                     EHIAALVAAVRGIHPKKRAPVRGVPLASRSRLV"
     gene            1030..1860
                     /gene="dapF"
                     /locus_tag="PK849_04730"
     CDS             1030..1860
                     /gene="dapF"
                     /locus_tag="PK849_04730"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPE65463.1"
                     /translation="MIPFVKMQGNGNDFIVLDNRDVRFSSDDLFSMARTYCRRRSSLG
                     ADGILVVENSQEADFTMRLFNADGSEGEMCGNGARCIARYAFEKKIAGNEQSFSTLAG
                     IMKAVVTPPFVDLDMGTVPLSSGWFNRPLSVGARSFDTSFLVVGVPHLVLFLEEDALT
                     REETIETGRELCHTYALFPNGTNVNFVVPNDDTHIASVTYERGVEDLTDSCGTGSTAS
                     AIAWVLRNAAVPSGTVVEVKNPGGVNFVTLSFDHSSEFVAASLKGRALMVAEGALFEN
                     "
     gene            1960..5400
                     /locus_tag="PK849_04735"
     CDS             1960..5400
                     /locus_tag="PK849_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HPE65464.1"
                     /translation="MKKLFIALVVLVALAGLAVATMDFAGDLVVREAGSAVKEYLGAD
                     LAVAGIKGNPVKGFSMQGVSLKKDGQPLFSAKFLDVSLNLASLLSKSPKLSLLSVGGV
                     EIDADRLAAQVAQLKFEGGGGGEIPIETVRFVESVVVSKWARADITKLGLSFKGKEIL
                     ADVDMAVNKVPVKGSTTALLDGENVDIRSLAMNVGKGSVSASGKVAPSLAVSGNLKEL
                     DLKELISFWPVVSAEGFDGAVSLSFRGDGEWNAPALAGDMEYAGKSLLGYPVESVKAK
                     WTLGRDKLSVEGLDARVLGMPLAGGMSLGFAAGKAPVVDLSLSGSGIKLAELKKVYPD
                     IGDVSGEVDKFTINLAGTMETLSGVIEFSAPKLGLMGYSVSESRAQVKLTPETATVSA
                     KSQFEGAPITAQGSINEYMTAPKLNLTINVRSLNLTKIASSFPQLKELALQGGANADV
                     WVKGTAAAPEVTVKVWSEKISAMKEAIESPTVMLSLKGETITISNAAAKWRGAAIAAS
                     GTVEGSEKLNITATLENIQPGAIAPFYPDIAQLKIKGAVTAKAVITGKPAAPKIDLTL
                     SSGSLGLLDSVSFKNLKATTSLVGDPKALEKADIDLNISAASAAAEGIGLSDLALKLK
                     KSGQKITILSAGAKSGAGSVSAAGAVALPAKAGEKGNLDLTVTIAKADLAFLAAAGGL
                     GVPLAGTLDGSVALKGALDAPSLVLKASSPKISVAGMTATDLSAALSGTMKDMKIDDF
                     KAKFGGGSLSAKGGVTLGATPAVTLDISGSDLDLAALVSGMPDAKELGIGGKLNVSFN
                     GRFAGAAGKGQGSISSPAFTVMGLKGSNLSYPIALDGNSISSKGASLSFYGGTLRGGG
                     ALDIKTMKFSHTVELNGVDVNSVAQDFTGGLEGKIGGLAKGSASISGALEPKLAYSGK
                     GSVTIGEGSITGFKAVQLLTALYRSGVRYSSVTAPFRLETGRFILEKGTKANAPQNDP
                     LYKFLTAEGPIGPKGALNLQCAGNVNLQVLNALTGGAVGGLTAGSLEDALKGLLGGLQ
                     SGMEKADFRDISFTLKGTTEKPGVSNLKIAPGAQQPGQPAPTGQPAEPQDPKKKLEQM
                     LIEQIVKPSKPAQPEQPQQMQPAQPDAPASSPTPAPTPTPEKKPEDIIKDKILESIFK
                     "
     gene            complement(5514..>6698)
                     /locus_tag="PK849_04740"
     CDS             complement(5514..>6698)
                     /locus_tag="PK849_04740"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPE65465.1"
                     /translation="GSLIEEYGLYGTGETLPVADTEEGWIIEMAPSPEGKGGLWVAKK
                     VQDGEIFVAANQFRIREVNPDDPDMMFSKNLHEIAQKHGWWKPEDGMLDWLRTVSLGE
                     YNHPYYSLRRVWRVLSLVAPSKNYSPWVEDGFTKDYPFSVAPDKKLTVRDVMRLHRDH
                     YEGTEFDLTKGAAAGPFGYPDRFYGPYDGAGDVGDPKRVLEGAWERPLSVTYCGYVFV
                     AQARSWLPSPVGGRMWLGLDKPSDTVFIPFHIGVNRLPSSVEVCDTTKFSRESAWWTF
                     NFLANYAGLKYSYMHKEIADMQDQLENGFLETLEIVERKAVDLHRTNPAGARTYLTEF
                     AERNTADTLKEWWNLAEHLIVKYNDGLLTEGGKIGRPLGYPKEWLKTTSWPEGPTNYR
                     KR"
BASE COUNT         1384 a         1905 c         2029 g         1380 t
ORIGIN      
        1 cacgaggcgg ctccggcttg ccagcggaac tccccggacg ggagcgcgtt tcttcgggtg
       61 gataccgcgt actgccgcaa cgagagcggc gatgtgctca ggagtggtgc cgcaacagcc
      121 gccgatcacc gtcgccccgg cctcggcaag gcgtttcccc catgttgcga agacctcggg
      181 ggagcggtac tcttcgttgc ccggaagtcc cgcgttcgcg tagacaaaga ccggaaggcc
      241 cgcgtgctct gacaggacct ctaccgtcga gatgtacgct tccggaccca cgccgcagtt
      301 ggcgcccact ccggcggcgc cctgataggc cgcccagcga gcggcaactt ccggcggggt
      361 tcccgtgacg gttctgccct tttggtcgaa cgtgaagctc accacaaacg cgccgtcggg
      421 atttacgtcc ttggcggcaa ggacagccgc cttggcctcc cggaggtcga tcatcgtttc
      481 gatgagaaga aagtccgccc cgccttccag aagcccctcc atctgcgtgc ggaaagaggc
      541 gtaagcctcg tcgaaggtca gtgagccgaa cggcgccaac agctcgccaa gcgggccgac
      601 ggaaccggcg acgagcgcgc gctctccggc tgcgctcttc gcgaggcgcg cagcccgtgc
      661 gttgatctcg cgcgcctttt ccctaagccc cctgtgggcc atttttatcg gggttccgcc
      721 gaaagtgttt gtctctatga tgcgcactcc ggcgtttata taggcggcat ggatgccggc
      781 gactacttcc ggactgtgaa tgttcatctc ctccggcagc atcggagggg tccacccctt
      841 ctccgcgagc agcgtcccca taccgccgtc aagcggcaca atcgcgcggt gttcttcgat
      901 ccagcggatg aattcttgtt tgttcatttt tcttccccct ctgcgctccg tataagttta
      961 tcaatatagc aaaaagaact gaagaaggat agtgtaaaat atagaaggaa aagaggagga
     1021 gggaaaacaa tgattccttt tgtgaagatg cagggtaacg gcaacgattt tattgtcttg
     1081 gataacaggg atgtgcgttt ttcatctgat gatctttttt ctatggcgcg aacgtattgc
     1141 cgcagacgaa gctcgctcgg agcggacggg atactcgttg tggaaaattc gcaggaggct
     1201 gattttacga tgcgtctctt caatgccgac ggcagcgagg gagagatgtg cggaaacggc
     1261 gcccgctgca tcgcgagata tgctttcgaa aagaagatcg caggaaacga gcagtcgttt
     1321 tcaactcttg caggtattat gaaagcggtt gtaacgcctc cgttcgtcga tctggacatg
     1381 gggactgttc ctctttcttc cggctggttc aaccgcccgc tctcggtagg cgctagatcg
     1441 ttcgatacat ccttcctcgt ggtcggcgtt ccgcaccttg tccttttcct tgaagaggac
     1501 gcgctcaccc gggaagagac aatagagacg gggcgcgagt tatgtcatac ctacgcgctt
     1561 tttcccaacg ggacaaatgt taactttgtc gtgccgaatg acgacacaca tatagcttca
     1621 gtaacctacg aaagaggtgt ggaggatctc accgattcct gcggcacagg ttcaacagcg
     1681 tccgctattg cgtgggttct tcggaacgcc gctgttcctt ccggaacggt cgtagaggta
     1741 aaaaacccgg gaggagtcaa tttcgtgacg ctcagcttcg atcactcaag cgagttcgta
     1801 gcggcttctc tcaagggacg cgccctcatg gttgcggagg gcgctctttt tgaaaattaa
     1861 agagatagaa tgttataatg agaaaagcat atcacgattc ggatggggga atgtagagga
     1921 cgcgtcgtca atgacgcgaa gaaagggagg tagagaacca tgaagaagct gttcattgcg
     1981 ttggttgttc ttgtagcgct cgccggactg gcagtggcga caatggattt cgccggcgat
     2041 cttgtggtac gggaggcagg ttccgccgta aaggagtatc tcggcgccga cctcgcggtt
     2101 gcgggaatca aggggaaccc tgtcaagggt ttctcgatgc agggagtatc gctgaagaag
     2161 gacggtcagc ctctcttcag cgcgaaattc cttgatgtct cgctcaactt ggcgtcgttg
     2221 ctctcgaagt cgccgaagtt gagcctgctc tccgtcggag gcgtcgagat cgatgccgac
     2281 cgtctggcgg cccaggttgc gcagttgaag ttcgagggcg gaggcggcgg cgagataccg
     2341 atagagacag tacgtttcgt cgagagcgtt gtcgtctcca agtgggccag agcagatatc
     2401 accaaactcg gcctctcctt caagggaaag gagattcttg ccgacgttga tatggcggtc
     2461 aacaaggttc ccgtcaaggg gagtaccacc gcgcttctcg acggtgaaaa cgtcgatatc
     2521 cgctcgctgg cgatgaacgt cgggaagggt tcagtatcgg cttccggaaa agtagccccc
     2581 tctcttgcgg tgagcggcaa tctcaaggaa ctcgatctga aggaactgat ctccttctgg
     2641 cccgtcgttt ccgccgaagg cttcgacggc gctgtgtcgc tctccttccg cggagacgga
     2701 gagtggaatg ccccggcatt ggcgggggat atggagtacg ccggaaaatc gctgctcggg
     2761 tatcctgtgg agagcgtcaa ggcgaagtgg acgctcggca gggacaagct gtccgtcgag
     2821 gggctggatg cacgggtgct cggtatgccg ctcgcaggcg gcatgtctct ggggtttgcc
     2881 gccgggaaag cgccggttgt cgatctttcg ctctccggat cggggataaa gctcgctgaa
     2941 ctgaagaagg tctatccgga cattggagac gtctcgggcg aggtcgacaa attcacgatc
     3001 aaccttgccg gaacgatgga gacgctttcg ggcgtgatcg agttcagcgc cccgaaactg
     3061 gggctcatgg ggtactcggt ttccgagtcc cgcgcgcagg tgaagctgac tcccgagacg
     3121 gcgaccgtga gcgcgaagtc gcagttcgaa ggcgcgccca taacggctca ggggtcgatc
     3181 aacgaataca tgaccgcgcc gaagctcaat cttacaataa atgtcagaag cttgaatctg
     3241 acgaaaatcg catcgtcctt ccctcagttg aaggagctgg cccttcaagg gggcgcgaac
     3301 gcggacgtgt gggttaaggg gactgccgcg gctcctgaag tcacagtcaa ggtgtggagc
     3361 gagaagatct cggcgatgaa agaggcgatc gaatcgccga cggtgatgct ctcgctcaag
     3421 ggagagacaa tcaccatatc gaacgccgcg gcgaaatggc ggggcgctgc gatcgccgcc
     3481 tcgggaaccg tcgaggggag cgagaaactg aatatcaccg cgacgctcga aaacatccag
     3541 cccggcgcca tcgcgccgtt ctacccggac atcgcccagt taaaaatcaa gggcgcagtg
     3601 actgcaaaag ctgtgatcac aggcaaaccc gccgctccga agatcgacct gacgctctcc
     3661 tctggcagcc tcggccttct cgacagcgtc tccttcaaga atctgaaggc gacgacatcg
     3721 ctggtcggcg acccaaaagc gcttgaaaaa gcggatatcg atctgaacat ctccgcagca
     3781 tcggccgcag cggaaggcat aggtctttcg gaccttgcgc tgaagctgaa gaagtccggg
     3841 cagaagatca cgatcctctc tgcaggagcc aagagcgggg caggttccgt ctccgccgcc
     3901 ggggcggttg cgttgccggc aaaagccgga gaaaaaggaa atcttgatct caccgtcacc
     3961 atagcgaagg ccgatctggc gttcctcgcc gccgccggag ggctgggagt tcctctcgcc
     4021 ggaacgctcg acggttcggt cgcgctcaaa ggcgctctcg acgcgccgtc gcttgttctc
     4081 aaggcctcct ctccaaagat ctccgttgcg ggaatgactg cgacggacct ttccgccgcg
     4141 ctttcaggca ctatgaagga catgaagatc gacgatttta aggcaaaatt cggcggaggg
     4201 tctctctctg cgaagggcgg cgttacactg ggggcgacgc ccgctgttac gctcgatatt
     4261 tcgggaagcg atctcgatct tgccgcgctt gtttccggga tgccggatgc gaaggagctg
     4321 ggcatcggcg gaaaacttaa cgtctccttc aacggacgtt ttgcaggagc ggcgggcaag
     4381 ggacaaggct ctatctcctc tccggcgttt actgtcatgg gcctgaaggg ctcgaacctt
     4441 tcgtacccga tcgctcttga cggaaactcc atctcctcga agggggcctc tctctccttc
     4501 tacggaggta ccctgcgagg aggcggcgcg ctcgacatca agacgatgaa gttctcgcac
     4561 accgtcgagc tcaatggggt ggatgtgaac agtgttgcgc aggatttcac aggcggtctc
     4621 gaaggaaaga tcggcggtct cgccaagggg tccgcgagca tctccggcgc gctcgagccg
     4681 aaacttgcgt actccggaaa ggggagcgtg accatcggag aggggagcat caccggcttc
     4741 aaagcggtgc agctcctgac tgcgctctat aggtcggggg tgcgctacag cagcgtgacc
     4801 gcccctttca gactggagac aggacgcttt attcttgaga agggaacaaa ggcgaacgcg
     4861 ccgcagaacg atcctctcta taagttcctc acggccgaag ggccgatcgg tcccaagggg
     4921 gcgctcaacc ttcagtgcgc ggggaatgtc aatcttcagg tgctcaacgc gcttaccgga
     4981 ggcgccgtcg gcgggctgac cgccggttcg ctggaagatg cgctgaaagg gttactgggc
     5041 ggcctgcaaa gcggtatgga gaaggccgat ttcagagata tttcctttac tttgaaagga
     5101 acgacggaga aacccggcgt ctccaatttg aagatcgccc cgggtgcgca acagcccggg
     5161 cagcccgctc ctacgggaca gccggccgag ccgcaggatc cgaaaaagaa gctggaacag
     5221 atgctcatag agcagattgt gaagccttca aagcctgcgc aaccggagca gcctcagcag
     5281 atgcagcctg cgcagcccga cgcgcccgct tcgtctccta cgcctgcgcc gacgccgact
     5341 ccggagaaga aaccggaaga tattatcaag gacaaaattc tcgaatccat attcaaataa
     5401 tagcggaaag aacgaacagg ggaggcgtac gcctccccct ttacgtatga tcaaaaactt
     5461 tccccgcgcg atggagagtt gtatccttca aagcgggatc agtcgatatg tgcttatcgc
     5521 ttcctgtagt ttgtgggtcc ctccggccat gatgttgtct tcagccactc tttcggatag
     5581 cccagaggcc ggccgatctt tcctccctcc gtcagcagcc cgtcgttgta cttcacgatg
     5641 agatgttcag ccagattcca ccactctttc agcgtatccg ccgtgttccg ttcggcgaat
     5701 tccgtgagat aggtcctcgc tccggccggg ttcgtcctgt gcaggtcgac tgctttgcgt
     5761 tcgacgatct ccagcgtctc caggaaaccg ttttcaagct ggtcctgcat atcggcgatc
     5821 tccttgtgca tgtagctgta cttgagcccg gcatagtttg cgaggaagtt gaatgtccac
     5881 cacgcgctct cgcgggaaaa tttcgtcgta tcgcagactt cgaccgagct cggaagacgg
     5941 ttgacgccga tgtggaacgg gatgaagacc gtgtcggacg gcttgtcgag accgagccac
     6001 atcctccccc cgacgggcga ggggagccaa ctcctggctt gggcgacgaa cacgtagccg
     6061 cagtacgtca cagaaagcgg acgttcccat gcgccttcca gcacgcgctt cggatccccg
     6121 acgtcgccgg cgccgtcgta agggccgtag aacctgtccg gatagccgaa cggtccggcc
     6181 gccgctccct tggtgaggtc gaactccgtt ccctcgtagt gatcgcggtg caggcgcatc
     6241 acgtcgcgca ccgtgagctt tttatcaggc gccacggaga aggggtagtc tttcgtaaaa
     6301 ccgtcctcga cccacggaga gtagttcttc gagggagcga ccagcgagag gacccgccag
     6361 acccttctaa gagagtagta cggatggttg tactcgccga gactcaccgt gcgcagccag
     6421 tcgagcattc cgtcctccgg tttccaccag ccgtgttttt gggctatctc gtggagattt
     6481 ttcgagaaca tcatatcggg gtcgtcagga ttcacctcgc ggatgcggaa ttggttcgcc
     6541 gcgacgaaga tttctccgtc ctgcactttt ttcgcaaccc agagaccgcc cttcccctcg
     6601 ggagagggcg ccatctcgat gatccacccc tcttcggtgt ccgcgacggg gagcgtctct
     6661 ccggtaccgt agaggccgta ctcctcgatc agcgaacc
//
LOCUS       DAOPKU010000109         6696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12146_length_6696_cov_5.263966, whole genome shotgun sequence.
ACCESSION   DAOPKU010000109 DAOPKU010000000
VERSION     DAOPKU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12146_length_6696_cov_5.263966"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK849_04745"
     CDS             complement(<1..195)
                     /locus_tag="PK849_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65466.1"
                     /translation="MSVSPMAGKRPDHTMLANIPALVASYYTVRPDTDDARSLVAFGT
                     SGHRGSSLKGSFNEAHIIAVS"
     gene            542..1900
                     /locus_tag="PK849_04750"
     CDS             542..1900
                     /locus_tag="PK849_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE65467.1"
                     /translation="MFSWKRIRYGVMFAFLAYISWVGYQHQVLGGGPAGIPTVDALCP
                     LGGLESIYSYLTSGVWLRRVAPSALILFIAVAGITLLLGRVFCGWICPLGTIGEFSNL
                     AAKKLGIRQRELPPALDRALKMLKYVVLAVILYFTWTWGTLAWREYDPWVSWMHLASG
                     WEEMVASPWGYVVLFFVVIGAGLFIERFWCRYLCPLGAALGILQKFSLTKVRRSKETC
                     ISCSKCNRSCPMGITPMALEKVTDADCIACGRCTESCPVEKTLQFSVAGKKVLSALAV
                     GLLGVGLYFAAYGTAKITGYWQTFASTSVEALKDPVDGVFGWMTIEQVAAQVHLPSAK
                     ILEIAGLPAESPTNVSIKQMEGVNDEELKEQLKAYFAANPAKAPEPKTVVPSNPDELK
                     GSFTLAEVASGYGLELSAILEKAGWPADADPNVKMRDVANTIGRDSSDIRNAVKELLE
                     NR"
     gene            complement(1973..2758)
                     /gene="xth"
                     /locus_tag="PK849_04755"
     CDS             complement(1973..2758)
                     /gene="xth"
                     /locus_tag="PK849_04755"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPE65468.1"
                     /translation="MARISIATFNVNSVRMRLPILEKWLKDRSIDVLCLQETKVVDED
                     FPVAAFKEMGYRAVFRGEKSYNGVAILTKVPMDGFAIGFGDGEEPECDTRLVRGRIGD
                     FCFINTYVPQGKAVDHADYVFKHRFLDRMKALLKREYLTSEKVVWVGDMNVAPTDIDV
                     TSPEKKRDHPCFAPDIQKHFEDVKSWGFVDLFRKYRPGEGEFSFFDYRVKNSLDRNIG
                     WRIDHVLVTPSLESAAADCFIDRDPRGWEQPSDHTPVVAVFDL"
     gene            complement(2772..3779)
                     /locus_tag="PK849_04760"
     CDS             complement(2772..3779)
                     /locus_tag="PK849_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPE65469.1"
                     /translation="MNNKMTDKTVVVTCAASESVRKGVEKGLDGAGKAVFLDDVPAER
                     RAAALSSAAVIMSFFFNKEVREEEYVLLEDVELFQSISTGLDFLPFRLMPQKMILACN
                     AGGWAPQIAEHTVALLLALTRRIVPLHNDLVKGVFGRHSYSPASLRGKVLTVVGYGGI
                     GRRTADLLRAFGIKIYALNTSGRTNDDVHFMGTLKELPEILPETDVLLLSIPLDKHTK
                     GLIGKRELDLMKKNAILLNVARAPLVVEEDLYNHLKANPEFQAGFDVWWKEPTWGGGD
                     FVLDHPFLDLPNVVGSPHNSNRCENDMENAVMAAAANVAAFFSGEKISGVIRREDYIE
                     D"
     gene            3869..4480
                     /locus_tag="PK849_04765"
     CDS             3869..4480
                     /locus_tag="PK849_04765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPE65470.1"
                     /translation="MESTLYLVRHGRPELPDNEMRFLGWTDLPLSSEGRRQAEATRDA
                     LSGYALDRIVHSGLKRAAETAGILREGREVPFEEIPAFRELAFGEWELRSMKELAENE
                     PELFAARGRDFARFRPPGGENFLDLHERVWPAFLELLGREKGNIMLVAHAGVIKTIIF
                     TLLGISWQKLFSLRQDYCGVHVISRWDEHLTVRQLNWTPRIGG"
     gene            4591..5733
                     /locus_tag="PK849_04770"
     CDS             4591..5733
                     /locus_tag="PK849_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65471.1"
                     /translation="MVRYGTRRGAWLCRGGIVLFGILAFIVLLLPLVSEAQQGLSASE
                     REYVERYNTLTPQQQQSLRDRMTPDQRTHLDRLLLMLSSPDRSGGASMTPAISAAAPS
                     GAQAKLTKKIIAGVPTQLTYTVVVQNRSGVPVVNPAVKDNGKPLNNDDAVKTGGNRDN
                     ILEIGETWTWTYTVTVTGKTGERILNTVTVEGPDSTIRDTDQGNNTDETVVEVDPAPG
                     NYDLSVSKTVAVTGQADTGLPPGINPTDVVDSPPQTITPDDCGKWLRSASGGYKGTRK
                     QYDIASLPSGTTFDLDFQAFDVPDKIEVYYPAGTKVFDSGWRGDMNHIQKKPSVANLY
                     PGGVVGTGKLLQNGLFTKSGANEFLVIVYGPEQGTAWEFSVKANCP"
     gene            5953..>6696
                     /locus_tag="PK849_04775"
     CDS             5953..>6696
                     /locus_tag="PK849_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HPE65472.1"
                     /translation="MASALETGALVPVSQKAAQDYAAHADNLLNYVDACMKKAPLDAL
                     AAKQVEMMLQNHRNHLQFMMSFFRLNNPELLKEIVIWVYRAYQAHGFSPDYFPAVLRR
                     WMEAVSQFLPPESAPEILGVYGWMLENHQRFLAAADEYRHMEQEAAGAPHPLQALRNE
                     FLQGLIAGSLSRCNACATGYITGKDELREFYLGVVQPAMIEIGNLWERGELSVAREHL
                     ATAIVTRIMAAQHMRFFGLEKPKGKALISA"
BASE COUNT         1416 a         1978 c         1966 g         1336 t
ORIGIN      
        1 gctgaccgca ataatgtgcg cctcgttgaa ggaacctttc agggaagatc ccctgtgccc
       61 cgacgttccg aaggctacga gcgagcgcgc atcgtccgtg tctggacgga ctgtgtagta
      121 ggatgcgacc agcgcgggta tgtttgccaa catcgtatgg tccgggcgtt tgccggccat
      181 cggcgatacg ctcatcgtga ttcccccctg ttcatcaaca tacatgtcga cccgggacgg
      241 gttctcgaca cggccggaag tcttccgcgc gcaacagctg ccgcacggga taacgaagca
      301 acaataccag gagattcaaa ggatattata ctagaaaatt ctctttcatt cacttccgta
      361 cctgcccgaa tccgcaggcg aagaggggat tgcactgcgc gcattctgtg catgagccta
      421 cggatgctcc tctctgctct cagtaatatg acgtaccccc ggagtgtata aagaaacaat
      481 agtattccag tccggatggt gctattctat tagaaaattc tcatcgaggg ggaggatctg
      541 catgttttca tggaagcgta tccggtacgg cgtcatgttc gcttttctgg cgtacatcag
      601 ttgggtgggg tatcagcatc aggtattggg aggaggaccg gcgggaatac cgaccgtcga
      661 cgccctctgt cctctgggag gccttgaaag catctacagc taccttactt cgggcgtctg
      721 gctccgccgc gtcgcaccca gcgcgctgat cctcttcatc gccgtcgcgg gcatcacgct
      781 gctgctcggc agggtcttct gcgggtggat ctgccccctg gggaccatcg gagaattcag
      841 taacctcgcg gccaaaaagc tcgggatacg gcagcgggag cttccgcccg cgcttgaccg
      901 cgctttgaag atgttgaagt acgtcgtcct cgccgttatt ctctacttta catggacctg
      961 gggaacgctt gcatggcgcg aatacgaccc atgggtctcg tggatgcacc tcgcctccgg
     1021 atgggaggag atggttgcct ctccgtgggg gtacgtcgtt ctcttctttg tcgtcatcgg
     1081 cgcggggctc ttcatcgaac gtttctggtg ccgctatctc tgccctctgg gcgcagccct
     1141 cggcatcctg cagaagttca gtttgacgaa ggtgcggcgc agcaaagaga cctgcatctc
     1201 ctgctccaag tgcaaccgct cgtgcccgat gggaataacg cctatggcgc ttgaaaaagt
     1261 caccgacgcg gactgcatcg cctgcggccg ctgcacggag agctgtccgg tcgagaaaac
     1321 gcttcagttc tccgtcgccg ggaagaaagt gttatcggcg ctcgcggtcg gactgctcgg
     1381 cgtgggcctc tacttcgccg cctacggcac ggcgaagatc accggctact ggcagacatt
     1441 cgcctccacc tcggtcgagg cgctgaagga tcctgtggac ggcgtcttcg ggtggatgac
     1501 catcgaacag gtggctgcgc aggtacacct tccgtcggca aaaattctgg agatagccgg
     1561 gcttcccgcc gagtcgccca caaacgtctc gatcaagcag atggaaggcg tgaacgacga
     1621 ggagctcaag gagcagctca aggcctactt cgcggcaaac ccggccaagg ctcctgaacc
     1681 gaagacagtc gtgccgtcca acccggacga actgaaggga agcttcacgc tcgcggaggt
     1741 ggcgtcgggg tacggtctgg aactctccgc catcctcgaa aaagcagggt ggcctgcgga
     1801 cgccgatccg aacgtcaaga tgcgcgacgt ggcgaatacg atcgggcgcg actcctcgga
     1861 catccggaac gcggtgaagg agctgctgga gaatcgctga tcgaacacgg gaggacggaa
     1921 acggaataag aaagcggggg aatctctccc ccgctttttc gtacctatga gcttacaggt
     1981 cgaagaccgc gacgaccggc gtatggtcgg agggctgctc ccaccctctg ggatcgcggt
     2041 cgatgaaaca gtccgccgca gcgctctcca gagagggcgt caccagcaca tggtcgattc
     2101 tccagccgat gttccggtcg agggaattct tcacccgata gtcgaaaaag gaaaactcgc
     2161 cttcgcctgg gcggtacttc cggaagagat cgacgaaccc ccagctcttc acgtcctcga
     2221 agtgtttctg gatgtccggg gcgaagcacg ggtgatcgcg cttcttctcc gggctggtga
     2281 cgtcgatgtc ggtgggggcc acgttcatgt cccccaccca gacgaccttc tccgaagtca
     2341 ggtattcccg tttcagcaac gccttcatcc tgtcgaggaa ccggtgcttg aagacgtagt
     2401 ccgcatggtc gaccgccttt ccctggggaa cgtaggtgtt gatgaagcag aagtcgccga
     2461 tgcgcccgcg gacaaggcgg gtgtcgcact cgggctcctc gccgtcgccg aagccgatgg
     2521 caaatccgtc catggggact ttcgtcagga tggcgacccc gttataggac ttctctccgc
     2581 gaaagacggc ccgatacccc atctccttga acgcggcgac gggaaaatcc tcgtccacaa
     2641 ccttcgtctc ctggaggcag agaacgtcta tcgaacggtc tttcaaccat ttctcaagga
     2701 tcggaagacg catacggact gagttgacgt tgaatgtagc tatcgaaatc cttgccatga
     2761 cggcactctc cttagtcttc gatgtaatct tcccggcgaa tgacgccgct gatcttctct
     2821 ccgctgaaaa aggccgcgac attggcggcg gcagccatga ccgcgttctc catgtcgttc
     2881 tcgcagcgat tcgagttgtg cggcgaaccg acgacattcg gcagatccag gaacggatga
     2941 tcgaggacaa aatccccgcc gccccatgtc ggctccttcc accagacgtc gaacccggcc
     3001 tggaactccg gattcgcctt caggtgattg tacaggtcct cctccacgac gagcggggct
     3061 ctggccacat tcaaaagaat cgcgttcttt ttcatcaggt cgagctcgcg cttgccgatc
     3121 aaccctttgg tatgcttgtc gagcgggatc gagagcagga ggacgtccgt ctccgggaga
     3181 atctccggaa gctccttgag cgtccccatg aaatgcacgt cgtcgtttgt ccggccgctc
     3241 gtgttcagcg cgtagatctt gatgccgaag gcgcgcagca gatcggcggt ccgccgcccg
     3301 atgccgccgt accccacgac cgtgagcacc tttccgcgca agctcgccgg cgagtaggag
     3361 tgccggccga aaactccctt caccaagtcg ttatggagcg gcacgatacg ccgggtcagc
     3421 gccagcagaa gcgccacggt gtgctccgcg atctggggcg cccacccccc cgcgttgcat
     3481 gcgagaatca tcttctgcgg catcaggcgg aaggggagga agtcgagtcc ggtcgagatg
     3541 gactggaaca gttcgacatc ttcaagaaga acatactctt cctcgcgaac ttctttatta
     3601 aagaagaagg acatgatcac cgcggcggaa gagagcgccg ccgccctcct ctccgcagga
     3661 acatcgtcga ggaagacggc ctttccggcg ccgtcgagcc ctttctccac accttttcgg
     3721 acactctccg acgcggcgca cgtaacgaca acagtcttgt cggtcatctt gttgttcatt
     3781 ttcccggttc tcctttcacg aaatgcgtgt ttctgttatt gtaccgtaaa acgacattga
     3841 aatgcaccgc ttcggaagga gtcttcaaat ggaatctaca ctttatcttg tgcgacacgg
     3901 acggccggag ctgcccgaca atgaaatgcg ctttctggga tggaccgatc tgccgctctc
     3961 ctccgaggga cggcggcagg cggaggcgac aagagacgcg ctctcgggat acgctctcga
     4021 ccgcatcgtc cacagcgggc tgaaacgtgc cgccgagacg gccggaattc tgcgcgaggg
     4081 acgtgaggtt cccttcgagg agattcccgc attccgggag ctcgcgttcg gcgaatggga
     4141 gctgcggtcc atgaaagagc tggccgagaa cgagccggaa ctcttcgcgg cgagggggag
     4201 ggacttcgcg cgcttccgtc cgccgggagg ggaaaacttc ctcgacctcc acgaacgggt
     4261 gtggcccgcc ttccttgaac tcctgggccg cgaaaagggt aatatcatgc tcgtcgccca
     4321 cgcaggcgtc atcaagacga tcattttcac gctgctcggc atttcctggc agaagctctt
     4381 ctcgctccgt caggactact gcggcgtgca tgtcatctcc cgctgggatg agcatctcac
     4441 ggtacgccaa ctcaactgga cgccccgcat cggagggtaa aagcgaacta tcgagagggc
     4501 gcgccgttac agatgacttg acagaagacg cgcttgttgc tattgtattc tgtaaagacg
     4561 cactattcaa aagaagggag gaatggcatc atggtacggt acggtacacg acgtggtgcg
     4621 tggctgtgca gagggggaat tgtacttttc gggattctcg ccttcattgt actgcttttg
     4681 ccccttgttt ccgaggcgca gcaggggctc tccgcttcgg aacgggagta tgtggaacgg
     4741 tacaatacgt tgacgcccca acagcagcag agccttcgcg acaggatgac ccccgaccag
     4801 agaacgcatc tggacaggct cctgttgatg ctctcctccc ctgaccgatc gggaggcgcc
     4861 tctatgacgc ccgcgatatc ggctgccgct ccctcgggtg cacaggcaaa actgacgaaa
     4921 aaaataatcg cgggcgtccc cacgcagctc acctacaccg tcgttgtaca gaaccggtcc
     4981 ggagtccctg tcgtcaaccc cgcggtaaaa gacaacggta aacctttgaa caacgacgat
     5041 gcagtgaaaa cgggcggcaa ccgggacaat atccttgaga tcggcgagac gtggacatgg
     5101 acgtacacag tcaccgtgac ggggaaaacc ggagagcgca tcctcaatac cgtcacggtg
     5161 gagggaccgg attctacgat ccgcgacacc gaccagggaa acaacacgga tgaaaccgtc
     5221 gtggaggtcg atccggcgcc ggggaactac gacctttcgg tctccaagac cgtcgccgtc
     5281 accggacagg ccgataccgg acttcctccg ggcattaatc cgacggacgt cgtcgactcc
     5341 ccgccgcaga ccatcactcc ggacgactgc ggaaagtggc tgcgaagcgc cagcggcggc
     5401 tacaagggaa cgcggaagca gtacgatata gcttctctgc cttcgggaac gaccttcgac
     5461 ctggacttcc aggccttcga cgtgccggac aagatagagg tttactatcc tgcaggaacc
     5521 aaggttttcg acagcggatg gcgaggggat atgaaccata tccaaaagaa accgagcgtg
     5581 gccaatctct atccgggcgg cgtggtcgga acggggaagc ttctgcagaa cggtcttttc
     5641 acgaagagcg gcgcgaatga attcctggtg atcgtctacg gcccggaaca gggaaccgct
     5701 tgggagttca gcgtcaaggc caactgtccc tagtcttggg agaaaaacgc tccaggacag
     5761 atgctatgaa attcgaaaaa agccctcggc gtccaaaact ctccggacgc agggggcttt
     5821 ttcctgaagc atgccgcgcc ggaggccggg aaaggaatac ggaacacgcg ggccggattg
     5881 acacccactt ctaaaaagcc taccataaca gctgttccat ccattttcgt ccacgagaag
     5941 gagttgagtt cgatggcatc cgcacttgaa acaggcgctc ttgttccggt ttcgcaaaag
     6001 gcggcgcagg actatgcggc gcatgcggac aatctgctga attatgtcga cgcgtgcatg
     6061 aaaaaagccc cgcttgacgc tctcgcggcg aaacaggttg aaatgatgct gcagaaccat
     6121 cgcaatcacc tccaatttat gatgtcgttc tttcgcctga acaaccccga actgctcaaa
     6181 gagattgtta tctgggtcta tcgggcgtat caggcccatg gattttctcc cgactacttc
     6241 cccgccgtcc tcagacgctg gatggaggcg gtttcgcaat tcctcccgcc ggagagcgca
     6301 ccggagatac tcggcgtcta cggctggatg ctggaaaacc accagcgctt tctcgcggct
     6361 gccgatgagt acagacacat ggaacaagag gccgccggag cgccccaccc gctgcaggcg
     6421 ctccggaacg agtttctcca ggggctgatt gcgggcagtc tctcccgctg caatgcctgc
     6481 gctaccgggt atatcactgg aaaagacgag ctgcgggagt tttacctcgg cgtcgtgcag
     6541 ccggcgatga tcgagatcgg caatctgtgg gaacggggcg agctttcggt agcgcgggaa
     6601 catctcgcaa cagccatcgt caccaggatc atggcggcgc agcacatgcg cttcttcggt
     6661 ctggagaaac ccaaggggaa ggcgctgatt tcagcc
//
LOCUS       DAOPKU010000110         6689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12162_length_6689_cov_5.793488, whole genome shotgun sequence.
ACCESSION   DAOPKU010000110 DAOPKU010000000
VERSION     DAOPKU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12162_length_6689_cov_5.793488"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /gene="leuS"
                     /locus_tag="PK849_04780"
     CDS             <1..1879
                     /gene="leuS"
                     /locus_tag="PK849_04780"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPE65473.1"
                     /translation="DLDNLPGWPDRVKTMQRNWIGRSEGARLTFTEKSTGEIIETFTT
                     RFDTIYGVTFLALAPEHPFVEKMCALSPKGDEIRAFVQKCVTQSAIERTAVGGEKEGF
                     FTGFSAVNPVTGESVPILIANYILMDYGTGAIMGVPAHDQRDFEFARKYDIPVKVVIQ
                     PHDESLDGVTMEHAFEDDGITCNSGSFDGLPTAEAIPAMIEWGVEQGFCSREVNFRLR
                     DWLISRQRYWGAPIPVVYCDCCGIVPVPEDELPVLLPAEVKIVEGGKSPLTEDKEWLT
                     VPCPKCGKPGRREADTMDTFICSSWYFFRYCSAREDGAVFNVDEANYWMAVDQYIGGI
                     EHACLHLIYARFFTKFFADLGLCHAREPFTNLLTQGMVIKDGAKMSKSLGNVVDPDEI
                     INTYGADTARLFILFAAPPANDLDWSERGVEGANRFLNRVWRLVEENIALLSSVRERP
                     SLPMERLSEQRMRDLKRKIHTTIRDVTRDIADEKQFNTAVARLMELLNALSSFRPETD
                     DESALFREGVDVLLACLNPFCPHITEELWSLGGHKETLSKLPWPEVEPSALEMDSVTV
                     VVQFNGKVREKEDFPAGLSKEELQERVFALPSVQKRLEGVEVVRVIVVPDKLVNVVVK
                     G"
     gene            1879..2814
                     /locus_tag="PK849_04785"
     CDS             1879..2814
                     /locus_tag="PK849_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65474.1"
                     /translation="MPHLRVIASGGASARRLVAEELEALSKKGRAFAGTHEGGEWPAL
                     IAAGRSAGLFDDKRVSVVESAELLGPFPDELLPYLEGDDASEVLLLVFEKEPGKTFSP
                     AAKKKIVQIKGESVPFWSAQRKKWLLDLAGKEGVALSDDAAALLVELLEDPEEVRAEL
                     RKLGSYAHGAPVDAGMVKELSFDEGRNTMLKFLDAFCQARAPEVFAALERFKQEPSML
                     PLLTGLYNRIRPALYLGIFAPAGGERIRSALEIKEYALKMGREALRRYSSKALTELSF
                     GLLVLSWKEKTSFAEGWPGFESLLMTCMASAEKRQ"
     gene            complement(2899..3180)
                     /gene="rpsT"
                     /locus_tag="PK849_04790"
     CDS             complement(2899..3180)
                     /gene="rpsT"
                     /locus_tag="PK849_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPE65475.1"
                     /translation="MPNKKSAEKRMKTSERNRLYNRFWTTRCKNAVKKVLEAVEGQDE
                     ALAQTRLAIAQSVIDKAVVKGVMHGNTAARRKSMITNRVKALSGEKQPA"
     gene            3291..5261
                     /locus_tag="PK849_04795"
     CDS             3291..5261
                     /locus_tag="PK849_04795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPE65476.1"
                     /translation="MMTIPSMDEFFGPEGLLNKRFSSFEYRKEQQDLAEEVHKALSAE
                     GEFILAAEAPPGVGKTFALLVPAMLRAAETGETVLVLTAGIPLQEQLIQKDLPLLNEL
                     LGLSLPFGLLKGKGNYACRSRVEEVFEGGEGRQGYLSHRDGGSSSRTVEEWLRTTKTG
                     DLSELSLPPNSPSVAGIAASSRSCRGFRCPRRGECFVQRVLREAREWRVVVANYHLFF
                     SYLLGAGKPFPAPFDLLICDEAHHLPEAARSSMTVSVSDDDVVRLLRSRVFTDTLGRL
                     EKSGRGVQNAAALSAEIRQESARFFELLDVLLPGRKENITVRNEEMLRQQRILSGKML
                     ELYNVFVPLVSDDETPDVSEDGGLSSWMEECGRIRRSLAWCAEVEKYPSWAYWKSERS
                     LLSAPVSPGEELSSGFFTTSAEKMIFISATLALGGKTDFWERETGIHPDRLFISGSPF
                     DLEQQMEILVVDTGLDVMNPRYDDTVCRIVEKLVEANGGSTLVLLASHRLLGKVGRAL
                     SSKPRGYRVLVQGEGPRSELLAAFRSDPSSVLVGSASFREGVDVPGDGLTQVIIDRIP
                     FPHPGDPLVQARNALEGAGAFVRVTLPEAKMLLKQAAGRLIRSRSDRGRVAILDGRVL
                     GRPEWKIPSALPKVKYRRLVVARSAVAPSGGV"
     gene            5334..5468
                     /gene="rpmH"
                     /locus_tag="PK849_04800"
     CDS             5334..5468
                     /gene="rpmH"
                     /locus_tag="PK849_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPE65477.1"
                     /translation="MKQTFQPHNRPRKRKMGFLARSSSPTGRRILRNRRRKGRARLAV
                     "
     gene            5472..5873
                     /gene="rnpA"
                     /locus_tag="PK849_04805"
     CDS             5472..5873
                     /gene="rnpA"
                     /locus_tag="PK849_04805"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPE65478.1"
                     /translation="MRFFFPRSSRLRRGWEFDSVFRTGDRLRGELVRLLFLKAEDGVT
                     RIGLAVGKRQGKSHDRNRGRRILKEGFRRILPWIRPGVWIVAGLSGTGMKAGSTDVYE
                     ELARLLKGGGFLEPEWPGANWENPSEGAKEK"
     gene            5870..6142
                     /gene="yidD"
                     /locus_tag="PK849_04810"
     CDS             5870..6142
                     /gene="yidD"
                     /locus_tag="PK849_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPE65479.1"
                     /translation="MTPAAWVGITLVRGYQLFVSPLLGHNCRFYPTCSQYAMDAISVH
                     GFLKGSFLAVKRLCKCAPWHPGGYDPVPPAARREEQGAYSYKDGDL"
     gene            6175..>6689
                     /locus_tag="PK849_04815"
     CDS             6175..>6689
                     /locus_tag="PK849_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPE65480.1"
                     /translation="MMRLMELVHGFTGSWGLAIIVLTLIVRLLLHPLTKKQMTSMQKM
                     QKLQPRIKMLQEKFKDDKESLNREMMALYKENKVNPAAGCLPLLVQLPVFILLYQVLT
                     NYDFSGVSFLWIRLDGSVLTTLGEALKLTVEKDQLGIMTVLYGLMANPYALLNVSVYL
                     PNLLLLLGIGFL"
BASE COUNT         1334 a         1852 c         2087 g         1416 t
ORIGIN      
        1 cgatctggac aacctgccgg ggtggcctga ccgagtgaag acaatgcagc gaaactggat
       61 cggccgttcc gaaggggccc gcctcacctt caccgagaag agtacaggtg aaatcataga
      121 aactttcacc acccgcttcg acactatcta cggagtgacc ttcctcgcac tggcgccgga
      181 gcatccgttt gtcgaaaaga tgtgcgcgct ctccccgaag ggagacgaga tcagagcgtt
      241 cgtccagaag tgcgtcactc agagcgccat agagagaaca gccgtcggcg gcgagaagga
      301 aggctttttc accggattct ccgcggtcaa tcccgtcacc ggcgagtcgg ttcccatact
      361 gatcgcgaac tacattctga tggactacgg taccggcgcc atcatgggcg ttcctgcgca
      421 cgaccagagg gacttcgagt tcgcgcgcaa gtacgatatt ccggtgaagg tcgttatcca
      481 gccgcacgat gaatctctcg acggcgtaac gatggagcat gccttcgagg acgacggaat
      541 cacctgtaac tcgggatcct tcgacggtct tcccactgcg gaggctatcc ctgccatgat
      601 cgagtggggc gttgagcagg gcttctgctc gagggaggtc aacttccggc ttcgagactg
      661 gcttatctcc cgccagcgct actggggcgc tcccattccc gtggtgtact gcgactgctg
      721 cggcatcgta ccggttccgg aggacgagct ccccgtgctc cttccagcag aagtgaaaat
      781 tgttgaaggc gggaaatcgc ctctgaccga agataaggaa tggcttaccg tcccctgccc
      841 gaagtgcggc aagcccggca ggcgcgaagc ggacaccatg gatacgttca tctgttcctc
      901 ctggtacttc ttccgctact gttcggcgcg cgaagacggc gccgtcttca atgtggatga
      961 agcaaactac tggatggccg tggaccagta catcggcggc atcgagcacg catgtctcca
     1021 cctgatctac gcccgcttct tcaccaaatt cttcgcggat ctcggtttgt gccatgcccg
     1081 cgagccgttc acaaatctcc tcacgcaggg gatggtcatc aaggacggcg cgaagatgtc
     1141 gaaatccctg ggcaacgtcg tcgacccgga cgagatcatc aatacctacg gcgctgatac
     1201 ggcgcggctc tttatcctct tcgcggcgcc tcctgcaaac gacctcgact ggtcggagcg
     1261 cggcgttgaa ggagcaaacc gcttcctgaa cagggtatgg cgtcttgtgg aggaaaatat
     1321 cgcgctgctc tcttcggtgc gggaacgccc ctctcttccg atggagcgtc tttccgaaca
     1381 gcggatgcgc gacctgaagc ggaaaattca cacgacaata cgcgacgtca cgcgggacat
     1441 cgccgacgaa aaacagttca atacggctgt cgcacggctt atggagctgc tcaacgcgct
     1501 ctcatccttc cgtccggaga cggacgacga gagcgcgctc ttccgggagg gcgtcgatgt
     1561 tctgctggcc tgcctcaacc ccttctgccc tcatataacc gaggagctgt ggtcgctcgg
     1621 cgggcataaa gaaaccctgt caaagcttcc atggcccgaa gtggagcctt cggcgctgga
     1681 gatggattcc gttaccgtcg tggtgcagtt caacggcaag gtacgcgaaa aagaggactt
     1741 cccggcgggg ctttcaaaag aggaattgca ggagcgcgtc ttcgcccttc cgtcggtgca
     1801 gaagcggctt gagggcgtcg aagtggtccg cgtcattgtg gtacccgata agctggtcaa
     1861 cgtggtggtg aagggctagt gcctcacctg agagtcatcg cgtcgggcgg cgcctccgcg
     1921 cgccgccttg tcgcggaaga actggaagcg ctgtcgaaga aagggagggc gttcgcggga
     1981 acgcacgagg gaggcgagtg gcctgcgctc atcgccgccg ggcgttccgc gggccttttc
     2041 gacgacaaac gcgtttccgt ggtggagtcc gccgagcttc tcggcccgtt tccggatgag
     2101 ctcctgccgt atcttgaagg tgacgacgcc tccgaggtgc tccttctggt tttcgagaag
     2161 gagccgggga agaccttctc ccccgctgca aaaaagaaga ttgtacagat caagggggaa
     2221 tccgttccct tttggtcggc ccagcgcaag aagtggctgc ttgatctcgc gggaaaagag
     2281 ggggtcgcct tgtcggacga cgcggcggcc cttcttgtgg aactcctgga ggacccggag
     2341 gaggtgcgcg ccgaactgcg aaagcttggt tcgtacgccc acggggcgcc tgtggacgcc
     2401 ggaatggtca aggagctctc attcgacgag gggcggaaca cgatgctcaa attcctggat
     2461 gcgttctgcc aggcgcgggc gcccgaagtg tttgccgcgc tggagcgctt caagcaggag
     2521 cccagtatgc ttcccttgct caccggactg tacaacagaa tacgccccgc gctctacctt
     2581 ggcatttttg ctccggcggg aggagagagg atccggagcg ccctggagat aaaggagtac
     2641 gcgctgaaaa tggggaggga ggcgctgcgg cgctattcgt cgaaggcgct gacggagctt
     2701 tcattcggtc tcctcgtgct ttcgtggaag gaaaagacct cttttgccga ggggtggccg
     2761 gggttcgaga gcctactcat gacctgtatg gcatccgcgg agaagagaca ataaaaaaag
     2821 ccgaacagcg gcgtcgcgcg ttcagcttta tagtgttcaa aatttcaaag ggaagaaaag
     2881 gaagagtgtg cgaaagaact acgcgggctg cttctccccg gaaagcgcct tcactctgtt
     2941 cgtgatcatg gattttcttc gtgccgctgt attgccgtgc ataacgccct tcacaacagc
     3001 cttgtcgatg acgctctggg cgatggcaag gcgcgtctgc gcgagagcct cgtcctggcc
     3061 ctccacagct tcgaggacct tcttcacggc gttcttgcac cgagtcgtcc agaaccggtt
     3121 gtaaagcctg ttcctttcgc tcgttttcat tcttttttct gcggatttct tgttaggcat
     3181 gtacctcacc tcctcccgat acaaccacga cattctagca cggtttctgt tatattagca
     3241 agagaggcgg agaaatttcc cgggaatttc gagtttcaga aagagggtac atgatgacaa
     3301 tcccatcgat ggacgagttt ttcggccctg aaggtctgtt aaacaaaaga ttttcgtcat
     3361 tcgaatacag aaaagagcag caggatcttg cagaggaagt ccacaaagcg ctttccgcgg
     3421 aaggtgagtt catccttgcg gcggaggcgc cgccgggggt tggcaagaca ttcgcgctcc
     3481 ttgttccggc gatgctccgc gccgccgaaa cgggggagac ggtgctcgtc ctcacggcgg
     3541 gcatccctct ccaggagcag ctgatacaga aggaccttcc cctgctgaac gaactcctcg
     3601 gactttcgct ccccttcggt ctgctgaagg ggaaggggaa ttacgcctgc cggtcacggg
     3661 tggaggaggt tttcgaagga ggagaaggca ggcaggggta tctttcgcac agggacggag
     3721 ggagctcgtc gcgtaccgtc gaggagtggt tgcggacgac gaagacgggc gatctctcgg
     3781 agctctcgct tcctccgaac tctccgtccg tcgcgggtat cgccgcgtcg tcgcgctcgt
     3841 gccgaggatt ccgctgtccg aggcggggag agtgctttgt gcagcgggtg ctccgtgagg
     3901 cgcgagagtg gcgcgttgtc gtcgccaact accatctctt tttctcgtac ctcctcggcg
     3961 cgggaaagcc tttcccggcg ccgttcgacc tcctgatttg cgacgaggcg catcatcttc
     4021 cggaagccgc ccgatcctcg atgaccgttt cagtgtcgga cgacgatgtg gtcaggctgc
     4081 tccggagcag ggttttcacc gacactctcg gaagactgga gaagagcgga cggggggtac
     4141 agaacgcagc ggcgctgtcg gcggagattc ggcaggagtc agcccgtttc ttcgagcttt
     4201 tggacgttct tctgcccggc aggaaggaga atatcaccgt tcgaaacgag gagatgctcc
     4261 ggcagcagag gatcctctcg ggaaagatgc ttgagcttta caatgtcttt gttccgcttg
     4321 tctccgacga tgaaacgccc gatgtgagcg aggacggcgg tctctcctcc tggatggagg
     4381 agtgcgggcg tatcaggcga agcctggcgt ggtgcgccga ggtggagaaa tacccttcgt
     4441 gggcctactg gaagagcgag cgctccctcc tcagcgcccc ggtgtctccg ggagaggaac
     4501 tttcgagcgg ttttttcaca acctccgccg agaagatgat cttcatctcc gcaacgcttg
     4561 ctctgggggg aaagaccgac ttttgggaac gggagacggg aattcacccg gaccgtcttt
     4621 ttatcagcgg ttctcccttc gacctcgaac agcagatgga gatcctcgtt gtcgataccg
     4681 gactcgacgt gatgaacccc cgctacgacg atacggtgtg ccgcatcgtt gaaaagctgg
     4741 tggaggcgaa cggggggagc acgctggtgc tcctggcgtc gcacaggctg ctcggaaaag
     4801 tcgggcgcgc tctgagctcg aaacccaggg ggtaccgcgt ccttgttcag ggggaaggtc
     4861 cgcgcagcga gcttcttgcg gcattccgga gcgatccgtc ctccgtgctc gtggggagcg
     4921 cctctttccg ggagggagtg gatgttcccg gagacgggtt gacccaggtc atcatcgacc
     4981 ggattccctt ccctcatccg ggggatcccc tggtgcaggc gaggaatgcg ctcgaagggg
     5041 cgggagcgtt tgttcgcgtc acgcttccgg aggcaaagat gcttttgaaa caggccgccg
     5101 gaaggctgat ccgaagcaga agcgaccggg gccgcgtcgc aattctcgac ggacgggtgc
     5161 tcggccgccc tgaatggaag atcccgtctg cgctgccgaa agtgaaatat cgtcgtcttg
     5221 tagttgcgcg ttccgctgtt gctccaagcg ggggtgtgtg atatagtatc aaggctgaaa
     5281 aaaagccgtt gatcagcggc tggttcttct attgccgtca ggaggtgtca ttggtgaaac
     5341 agacgttcca accccacaac agaccacgaa aaagaaagat gggtttcctc gcccggtcga
     5401 gttcgcccac aggccgtcgt attctccgga atcgcaggag aaagggccgc gcccggctgg
     5461 ccgtttaatc cttgagattt ttttttcctc ggtcttcacg ccttcgaaga gggtgggagt
     5521 tcgatagtgt cttccgcacc ggtgatcggc tgagaggcga actggtgcgg ttgctgtttc
     5581 tgaaggcgga ggatggcgtt acacgcatcg gcctggcggt gggcaaacga caggggaagt
     5641 cccacgaccg gaatagggga cggagaatat taaaagaagg cttccgcaga attcttccct
     5701 ggatccggcc gggagtatgg atagtcgcgg gactcagcgg cacgggaatg aaagcgggct
     5761 cgacggatgt ctacgaagag ctggcccgac ttctcaaagg gggagggttt ctggagccgg
     5821 aatggccggg agcgaactgg gaaaatccgt cggagggcgc aaaagaaaaa tgacgcccgc
     5881 tgcatgggtc ggaattacac tcgttcgggg gtaccagctt tttgtgtccc cgctcctcgg
     5941 gcataactgc agattctatc cgacgtgctc gcagtatgcg atggacgcga tttccgttca
     6001 tgggttcctg aaaggtagct ttttagcggt gaaacggctt tgcaagtgcg ccccgtggca
     6061 ccccggaggg tatgatcccg taccgccggc ggctcgaaga gaggagcagg gggcttattc
     6121 gtacaaggat ggtgatctat agtgggttct atttggaaga tgggcgggga ccttatgatg
     6181 aggctgatgg agcttgttca cggcttcaca gggtcctggg ggttggcaat catcgtactc
     6241 acgctgattg tccgtttgct tctgcacccc cttacaaaga agcagatgac gagcatgcag
     6301 aagatgcaga aactccagcc ccgtatcaag atgctgcagg agaagttcaa ggacgacaag
     6361 gagtcgctta accgcgagat gatggcgctc tacaaggaga acaaggtaaa cccggccgcc
     6421 ggatgccttc ctcttcttgt ccagcttccc gtcttcattc tgctctacca ggtgctgacc
     6481 aactacgact tttcaggcgt ctctttcctc tggatacggc ttgacggttc cgtgctcact
     6541 acgctcggcg aggcgctcaa gctgactgtc gagaaggatc agctcggcat catgaccgtg
     6601 ctctacggtc taatggcgaa tccgtatgcg ttgttgaacg tttccgtcta tcttcccaac
     6661 cttctgctgc tcctgggaat cggattcct
//
LOCUS       DAOPKU010000111         6659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12248_length_6659_cov_5.736523, whole genome shotgun sequence.
ACCESSION   DAOPKU010000111 DAOPKU010000000
VERSION     DAOPKU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12248_length_6659_cov_5.736523"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PK849_04820"
     CDS             <1..784
                     /locus_tag="PK849_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65481.1"
                     /translation="LFPHMNVERNIRWGLRFAALPDDAHVHRLIDLLKLKPLLGRSPE
                     TLSGGEQQRVALARALAVKPSVLLLDEPLSALDPSFREDLRDYLAEINRGGMTLVMVS
                     HDFGEVLSLGNRVAVISDGVLQQVGDVRSVFRAPANRSVAEFVGMKNLFPASIRGTDA
                     VTEGGATLLLGKAAASGERLLGIRPEDVVIGAEHPGDSRNAFQGKVVSIVPRGMAFEV
                     IMRTDGGMRITARVLSSALLSGGVAVGGEYWASFSPDSVHVL"
     gene            818..1762
                     /locus_tag="PK849_04825"
     CDS             818..1762
                     /locus_tag="PK849_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPE65482.1"
                     /translation="MKQLRRLFLAAAVLLMVSGAFAAEKVVVYHAGSLAAPMAKIEAM
                     YEEANPGVDIQRESGGSAALARKITDLGGACDAFFSADYMVIEKLLRPAAADWNILFA
                     SNAIVLMYGPKSKYGEEIGAKNWTEVMLRPDVRWGHSEPDADPCGYRSLMVLQLAEKF
                     YGDEALYERAMKHSGRGVRQKAIELVSMVESGAMDYAFEYKSVAVQHGLKYLDLPAEI
                     NLMEPSRADDYAAATVELAGKEPGKKMLVKGEPIVYGLSIPKDAPNADGAMKFVEFVL
                     SPKGGLAVFQEMGQDIVGPKAMGAGDSIPAVLKALLKN"
     regulatory      1895..2015
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2070..2765
                     /locus_tag="PK849_04830"
     CDS             2070..2765
                     /locus_tag="PK849_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE65483.1"
                     /translation="MDYLSEGFLQGFSLIFSGNEEVWSAVAVTLKLSGLSMLVTLLFG
                     IPAGFALGYFTFPGKSLLRLLVDTLLSIPTVVVGLFVYAFISNRGPLGQFELLFTIPG
                     MAIGQTILALPIVLALTASAVESADSRLRFTLLTLGARGRRLLFSTLWEGRHQILLAA
                     VTAYGRVTAEVGVSMMLGGNIKWHTRTVTTAITLETGKGEFSTGIALGVLLLCLSFVL
                     NWAVSFLRRRGAA"
     gene            2794..3450
                     /locus_tag="PK849_04835"
     CDS             2794..3450
                     /locus_tag="PK849_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65484.1"
                     /translation="MHKTPLYELTNIVCAYEDEPVLQVDSLAIPDEGPVAFVGHNGSG
                     KSTLLKLLSFLLPQVSGELRFLGETQDKKDDARLHATLLLQEPYLLRRSVFENIAYGL
                     RARGEASLRERVVEALEMVGLSADFARREWYQLSGGEARRVALASRLALRTRVLLLDE
                     PTANVDEENAQLMAEAVLDAWKRWNTTPLIATHDLQWLDTVTQRRIFMRRGKIERIAL
                     "
     gene            3662..4411
                     /gene="modA"
                     /locus_tag="PK849_04840"
     CDS             3662..4411
                     /gene="modA"
                     /locus_tag="PK849_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE65485.1"
                     /translation="MKLYRNRWVFSILAFFLVFGTALPSSAENAETVAAAASLQSLMA
                     KTIPWFNEQRGTGLQGVYGASGNLARQIESGAPFDLFLSADEKWARYVEEKGLLDGEP
                     LPFAAMPLVLWHSGETAPSLEILRSEKLTVAIANPETAPFGRRAKAYLMEKGLFEAIE
                     TSGRLIIGGDVLKTGLAAKSGGADLAILPLSTASSLGEGSWTLLDAEPQTLFGGVVKG
                     RSTPAIEAFWTFLRSPEAAPFLKEFGFLPPR"
     gene            complement(4467..4676)
                     /locus_tag="PK849_04845"
     CDS             complement(4467..4676)
                     /locus_tag="PK849_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65486.1"
                     /translation="MAPPHAPVKHDRPGMSFFAQMLIVLLVLALLLLLVLVADTYLHD
                     IGKDWSHSTLVKMILERREVVPLFE"
     gene            5038..5688
                     /gene="modB"
                     /locus_tag="PK849_04850"
     CDS             5038..5688
                     /gene="modB"
                     /locus_tag="PK849_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HPE65487.1"
                     /translation="MLPPLLLSLRVLLFAVPLLVLLGGGIGWLLARANFPGKGFVSLL
                     VQLPLILPPSVMGFYLLFAIGRNPFLKSLGILFSFPALVLAATFSALPIMIQSARAAF
                     ASVDGELEDAARTLGKGEWTVFRTVTLPLARRTLLVGLPLSAARALGDFGVSLMIAGN
                     IPGRTQTLPLYIYNRVETLDFAGAHVASLLLVGTGFASLYLVRRLEEGRNERRAAC"
     gene            5675..>6659
                     /locus_tag="PK849_04855"
     CDS             5675..>6659
                     /locus_tag="PK849_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65488.1"
                     /translation="MLHAEFADSFGNFSLRVSLTLGMETGVLFGRSGSGKSMTLRTLA
                     GLRTPSEGSVLLDERTLYSSAERINLPPRERNVGLLFQNLALFPHMTALDNVAYALPP
                     GKKGREHAARWLARMRMEGFEDRLPARLSGGQRQRVALARALAAEPKLLLLDEPFSAL
                     DGPLRRSLRRELREIRAEAGIPILYVTHQIDDLCSLGDRVFVLRDGQTAESFPVERLW
                     EKGARGEAWSALGWGNLFRGRITPCGSECACRFEGERVSLSLDGGAHAPGESLVFVAP
                     DKIRILYPDIPVDPDLGGNIFSAVVEETAPLGGSIRLYLASENGVLKRRSLF"
BASE COUNT         1161 a         2111 c         2112 g         1275 t
ORIGIN      
        1 gctctttccg cacatgaacg tggagcggaa tatccgctgg ggcctccgct tcgccgccct
       61 ccccgacgac gcgcatgtac accgtcttat cgacctgctg aagctgaagc cgctgctcgg
      121 gcgatctccg gagacgctct ccggggggga gcagcagcgc gtcgcgctcg cccgcgcgct
      181 cgccgtgaag ccctccgtcc tgctgctcga cgagccgctc tccgcgctcg atccgtcctt
      241 cagggaggat ttgcgggact acctggcgga gatcaatcgc ggcggtatga cgctggtgat
      301 ggtgagccac gatttcggcg aggtactttc gctcgggaac agggttgccg taatctcgga
      361 cggcgttttg cagcaggtag gggatgtccg gtcggtcttc cgggcgccgg cgaacagaag
      421 cgtcgccgag ttcgtcggga tgaagaatct cttcccggcg agcattcggg gcacggacgc
      481 cgtaacggag gggggcgcga cgctcctcct cggcaaggcg gcggcttccg gcgaacggct
      541 gctcggcatc cgccccgagg acgtcgtcat cggcgccgaa caccccggag actcccggaa
      601 cgcgtttcag gggaaggtcg tctccatcgt tccaaggggg atggcgttcg aagtgattat
      661 gcgcacggac ggcggcatgc gcatcacggc acgggttctt tcgtcggcgc ttctctcggg
      721 aggagtcgcc gtcggcggcg aatactgggc gagtttcagc ccggattcag tccatgtttt
      781 gtagatggtg catcatactt taacggaggt gttgttcatg aaacagttac gaaggttgtt
      841 ccttgcggca gcggttcttc ttatggtctc cggcgcgttc gcggcggaga aggtcgtcgt
      901 ctaccatgcg gggagccttg ccgctcccat ggcgaagatc gaggcgatgt acgaagaggc
      961 caaccccggc gtggacatcc agcgcgagtc gggcggcagc gcggcgctcg cgcggaagat
     1021 caccgatctc ggcggagcgt gcgacgcgtt cttctcggcg gactacatgg tcatcgagaa
     1081 gctgctccgg cctgcggcgg cggactggaa catcctcttc gccagcaacg ccattgtcct
     1141 gatgtacggg ccgaagtcga agtacggcga ggagatcggc gcgaagaact ggacggaagt
     1201 catgctgcgc cccgacgtgc ggtgggggca ctccgagccc gacgcggatc cctgcggcta
     1261 ccggagcctg atggtgctcc agctcgctga gaaattctac ggcgacgagg cgctctacga
     1321 gcgggcgatg aagcactccg gccgcggcgt ccgccagaag gcgatcgagc tcgtctccat
     1381 ggtggagagc ggcgcgatgg actacgcgtt cgagtacaag tccgtggccg tccagcacgg
     1441 actgaaatac cttgatctgc cagcggagat caacctgatg gagccttcca gagccgacga
     1501 ctacgcggcg gcgacggtcg aactcgcagg gaaggagccg gggaagaaga tgctcgtcaa
     1561 gggcgaaccg atcgtctacg gcctctcgat cccgaaggac gcccccaacg cggatggcgc
     1621 catgaaattc gtcgagttcg ttctctcgcc caagggcggc cttgccgtct tccaggagat
     1681 gggacaggac atcgtcggcc cgaaagcgat gggcgccggc gattcgatcc ctgccgtact
     1741 gaaagcgctg ctgaagaact gacaatcatt cgaagtgcgc agagggaatg ccccacgttc
     1801 cccctgcgca cttcgccgaa acggctgccg ttccccttcc cccgactaat tttcacattc
     1861 tttcttgctt ccccgacaga gaaaagatac aatagcaggg aacttcctcc gagcctcagg
     1921 accttcaggc gtttcgccca cggtcttccg gccgaaaggg tgtttccgga aacggagacg
     1981 cctcccgtgt ggaaaggggg gacaaggccg ccgcttcgac gtgccttctc cgcgcccgcg
     2041 ggggagacgc gacgaagggg tgtatatttt tggactatct ctccgaagga tttctgcaag
     2101 ggttctcgct catcttctca ggaaacgagg aggtttggtc ggcggttgcg gtgacgctga
     2161 aactctccgg cctttcaatg ctggtgacct tgctcttcgg catcccggcg ggattcgccc
     2221 tcggctactt taccttcccc ggcaagagcc ttctccgtct tcttgtagac acgctcctct
     2281 cgataccgac cgtggtggtc ggccttttcg tctatgcgtt catctcgaac cgggggcctt
     2341 tgggtcagtt tgaactgctc ttcacaattc ccggcatggc gatcggccag accatactgg
     2401 cgctccccat cgtcctcgcg ctcaccgcct ccgcggtcga gagcgccgac agccgtcttc
     2461 gattcacgct gctcacgctc ggtgcacggg gccgccgcct tctcttctcc acgctctggg
     2521 aggggcggca tcagatactg ctcgcggccg tcacggccta cggcagagtg accgccgagg
     2581 tgggcgtctc gatgatgctc ggcggcaaca tcaagtggca tacgcggacc gtcaccaccg
     2641 ccatcacgct ggagacgggc aagggagagt tctccacggg catcgccctc ggcgtgctgc
     2701 tgctctgcct ctcgttcgtc ctcaactggg ccgtttcctt cctgcgccgg aggggcgcgg
     2761 cgtgatcccc gccgaaagga tgtgcgcgct gtgatgcaca agacgccgct ctacgaactt
     2821 acaaacatcg tctgcgccta tgaggacgag cccgttctgc aggtggattc gctcgccatc
     2881 cccgacgaag ggcccgttgc gttcgtcgga cacaacggaa gcggcaaaag cacgctgctg
     2941 aagctgctct ccttccttct gccgcaggtg tcgggcgagc tgcgcttcct gggggagact
     3001 caggacaaaa aagacgacgc ccgtctgcac gcgacgcttc tccttcagga accctacctc
     3061 ctccgccgtt ccgtcttcga gaacatcgcc tacgggctgc gggcgcgggg ggaggcatca
     3121 ctgcgggagc gcgtggtcga ggcgcttgag atggtggggc tctccgccga cttcgcacgg
     3181 agagagtggt accagctctc tggaggagag gcccgccgcg tcgcgctcgc ctcgcgcctg
     3241 gcgctgcgga cgagagtgct tcttcttgac gagccgaccg cgaacgtcga cgaggagaac
     3301 gcgcagctca tggcggaggc cgtcctcgac gcctggaagc gctggaacac cacgcctctg
     3361 atcgcgacgc acgacctcca gtggctggac acggtgacac agcgaaggat cttcatgcgc
     3421 cgggggaaga tcgagcgtat cgcgctgtaa aacaaaaggg ccttcgcgct ccagacaacc
     3481 gggagaaatt ccctgccctc gaaaagtgcg gagaggggct ggagaaagag agaagttagg
     3541 gggaggattc tttgaaatcc ttcccttttt tcggtcgcca tactttttcg caaccgagtt
     3601 gcattttttg cgaacgaagc ctagaataag cgcaactttg ttgcaaaagg gaggaacagg
     3661 tatgaaattg tatcgaaaca ggtgggtttt cagcattctg gctttctttc tcgtattcgg
     3721 cacagcgctc ccctcgtcgg cggagaacgc ggagacggtt gcggcggcgg cctcgctgca
     3781 atccttgatg gcaaaaacca tcccctggtt caacgaacag cgcgggaccg gactccaggg
     3841 ggtgtacggc gcttcgggca atctggcgcg gcagatcgag tcgggcgcgc ccttcgatct
     3901 ttttttaagc gccgacgaaa aatgggcccg ttacgtcgag gagaaggggc tgctcgatgg
     3961 agagccgctc cccttcgcgg cgatgcccct tgtgctctgg cactccggag agacggcgcc
     4021 ctctcttgag attctgcgct cggaaaagct caccgtggct atcgcgaatc cggaaacggc
     4081 gcccttcggc aggcgggcaa aggcgtacct catggaaaaa ggactttttg aagcgatcga
     4141 aacgagcggc agactgatca tcgggggcga cgtgctcaag acggggctgg cggcgaaaag
     4201 cggcggcgcg gatctcgcca tcctgccgct gagcaccgcc tcctcgctcg gcgaaggatc
     4261 gtggacgctg ctcgacgcgg agccgcagac gctcttcggc ggggtcgtca agggacgaag
     4321 cactccggcg atcgaggcgt tctggacgtt cctccgctcg cctgaagcgg cgccgttcct
     4381 gaaggagttc ggtttcctcc cgcctcgttg aacactcgga atcgaaaagt gaactcacga
     4441 cttcagttca cgcgggagcg gaggggctac tcgaaaagag ggacgacctc cctccgctcc
     4501 aaaatcatct tcacgagcgt cgagtgcgac cagtctttcc cgatgtcgtg cagatacgtg
     4561 tcggcgacga ggacgagcaa gaggagcagc gcgaggacaa gcagaacgat cagcatctga
     4621 gcgaaaaagg acattcccgg gcggtcgtgc ttcaccggag cgtgtggcgg cgccattgcg
     4681 atcacatcct tgacggcatt cttgaaaaat agtcttccgg agcatacact ccggtacggc
     4741 gaaagaacac ccgtgaacat gccggaaggt cttccgctac cactattcta caacatgccg
     4801 cgcgcaatcg gcggaacggc gcgctatgcc gtaccgcctc cgtccgttct tcgatctgaa
     4861 acgattttga tgcaacgccg ttttcctgat accatgagca cattctgcat ttctggaggt
     4921 ctgccgcatg ttcgatgaac ttttcctctc ctctctcttc cggatatcgg ggctcgcccc
     4981 gctctatccc ctctgttccg ggcgctccac ggctcgcgcg agcagagcgt gacggcgatg
     5041 ctccctccgc tcctcctcag cctccgcgtc ctgctgttcg ccgttcccct gcttgttctc
     5101 cttggaggag ggatcggctg gctgcttgcg cgggctaatt tccccgggaa ggggttcgtc
     5161 tcgctcctcg tacagcttcc gctgatcctc cctccctcgg tgatgggctt ctacctgctc
     5221 tttgccatcg gaagaaaccc cttcctgaag tcgctcggca tccttttctc cttcccggcg
     5281 ctcgtcctcg cggcgacatt ctccgcgctc cccataatga tccagtcggc gcgggcggcc
     5341 ttcgcctcgg tggacggaga attggaggac gcggcgcgca ccctcggcaa gggggagtgg
     5401 accgtctttc gcaccgtcac gcttcctctc gcccggcgca cgctccttgt cgggctccct
     5461 ctctcggcgg cgcgcgccct gggagatttc ggcgtctcgc tgatgatcgc gggaaacatc
     5521 cccggcagga cgcaaaccct ccccctctac atctacaacc gtgtggagac gctcgacttc
     5581 gcgggcgcgc acgttgcgtc gctcctcctg gtcggcacgg gattcgccag cctctacctg
     5641 gtcagacgac ttgaggaagg gaggaacgaa cgccgtgctg catgctgagt tcgccgactc
     5701 cttcgggaat ttttccctgc gcgtctcgct cacgctggga atggagacgg gggttctctt
     5761 cggcaggagc ggctccggga agagcatgac gctccggacg ctcgccgggc tcagaacgcc
     5821 ctcggagggg agcgtcctcc tcgacgaaag gacgctctac tcgtcggcgg agcggatcaa
     5881 cctccctccc agagagcgga acgtaggact gctcttccag aacctcgccc tctttccgca
     5941 catgacggcg ctcgacaacg tggcgtacgc cctgcctccg ggcaaaaagg ggcgggaaca
     6001 cgccgcccgg tggctcgccc ggatgcggat ggaggggttc gaggaccggc ttccggcgcg
     6061 gctctccgga ggacagcggc agcgagtggc actggcgcgc gccctggccg cggagccgaa
     6121 gctgctgctc cttgacgaac cgttcagcgc cctcgacggt ccgctgcgca gaagcctccg
     6181 gcgcgagctg cgcgagatcc gcgccgaggc gggcattccc atcctctacg tcacgcatca
     6241 gatagacgac ctctgctcgc tcggcgacag ggtgttcgtc ctccgggacg ggcagaccgc
     6301 ggagagcttc ccggtggaac gcctctggga gaagggcgcg cgcggcgagg cgtggagcgc
     6361 gctcggctgg ggaaacctct tccggggcag gatcaccccc tgcggatcgg agtgcgcttg
     6421 ccgcttcgag ggggagaggg tcagcctctc gctcgacggg ggagcgcatg cgccgggaga
     6481 gagccttgtg ttcgtcgccc ccgacaagat ccgcatcctc tacccggaca ttccggtcga
     6541 cccagacctc ggcggcaaca tcttctccgc cgtcgtcgaa gagactgcgc ctcttggggg
     6601 aagcatccgg ctctatcttg cctcggagaa cggcgtcctc aagaggcgca gtctcttcg
//
LOCUS       DAOPKU010000112         6653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12267_length_6653_cov_4.367839, whole genome shotgun sequence.
ACCESSION   DAOPKU010000112 DAOPKU010000000
VERSION     DAOPKU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12267_length_6653_cov_4.367839"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PK849_04860"
     CDS             <1..812
                     /locus_tag="PK849_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65489.1"
                     /translation="RLRGFAPGRFSFNVKGGRCEACGGGGVTKVSMLFLPDVYVPCEV
                     CGGKRYNHETLEVKFKGKSIADVLDMTVDEAYAFFRDIPRIASKLQLIAEAGLGYIRL
                     GQSALTLSGGEAQRVKLSKELSKRFSGPTLYLLDEPTTGLFYTDVEKLLHIINKIVDN
                     GNTVILIEHNLDVLLSSDYIVDLGPEGGSGGGRLVATGTPEDIMTGEKGHTAYFLQEY
                     AKQIAERVKKSGKKNGPNSGQKSSKRAEKRGETELSQISSTERRTKRREAK"
     gene            871..1551
                     /gene="rlmB"
                     /locus_tag="PK849_04865"
     CDS             871..1551
                     /gene="rlmB"
                     /locus_tag="PK849_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPE65490.1"
                     /translation="MQKVYLGKNVSDRFSLQIGDLAEKGKVTVLRVTPEALTDMCGTN
                     NHQGVACRSGDVSMLELQDILPLLDGKKGPVLLVLLDHVQDPHNLGAVVRTAEACGAM
                     AVIFPKRRAALPGGTVVKVSAGAALRVPMVAVTNVAQTVREIQQNGFWTIGLDNKNGR
                     SLWSETMPEKTLLVVGAEGEGLSRLVAETCDETVRIPVAGATGSLNASVACAVGMFEW
                     ARKWGAQS"
     gene            complement(1742..2221)
                     /locus_tag="PK849_04870"
     CDS             complement(1742..2221)
                     /locus_tag="PK849_04870"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HPE65491.1"
                     /translation="MREEDETLLRKSVDAMARALALYHGIFAREAVGMLGQEKGTALV
                     ENVVRQFGLERGRRVREQALAAGDALDLKGMNEHYDLPLAYAWDALKQENGSDVSFCP
                     MADEWKRNGMVEEGKLYCAVDYAIAEGFSPDLHFSRSSSLMHEDSCCCHRYSNGFKK"
     gene            complement(2264..3235)
                     /locus_tag="PK849_04875"
     CDS             complement(2264..3235)
                     /locus_tag="PK849_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HPE65492.1"
                     /translation="MYYLNAESMISSFSFSGVMDAVANAMIQIETSPNSLIAPLRNSV
                     SLEKNTLMMMPCLSEKEWGLKVLTLFPDNPVRLKPFINGLFLLFDGSDGSPSALLDGR
                     TLTAIRTGGVGGLAVRTLASTHATRLGIVGAGEQSYWQVRFACSVRPFSQVILYDTIE
                     SKAEFCAERIRKVLPELEIEITKDTTFLLKHSDVVITATNTTTPVFEDSPMYFEGKTF
                     VGIGSYRPDMREYPDALFRAISNIFVDTEHAFDETGDLIEPLQSGVVSKKNIRSMGAF
                     LSREKADFTAESGAFFKSVGYAAFDLFVARFLFQRGVENGKAIDLGI"
     gene            3689..4063
                     /locus_tag="PK849_04880"
     CDS             3689..4063
                     /locus_tag="PK849_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPE65493.1"
                     /translation="MDEKVGPGGKGSKLALIEEHPILDFVHGEKVRFFFDGKELEGFE
                     GEPISMALHANGVRIYRETAEMHRPRGFFCAIGKCSSCFMIVDGVPNVRTCITPLKAG
                     MKVETQHGKGNVPDLNDGRPRS"
     gene            4060..5169
                     /locus_tag="PK849_04885"
                     /pseudo
     CDS             4060..5169
                     /locus_tag="PK849_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713128.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            5162..5674
                     /locus_tag="PK849_04890"
     CDS             5162..5674
                     /locus_tag="PK849_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPE65494.1"
                     /translation="MSDKNMDMLFNSGVLTGEREGAILPPRELWEKKKGGVVIIECPQ
                     RIPCNPCHTSCPTGAILPFEDINNTPKVNWEKCTGCTLCVSACPGLACFVIDLTFSEE
                     NALYKLPYEMLPLPVKGQKVQCLDREGKSVGEGTVENVTQPRKDRTHVVHVSAPKSLV
                     TIFRSIRVVK"
     gene            5674..5964
                     /locus_tag="PK849_04895"
     CDS             5674..5964
                     /locus_tag="PK849_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPE65495.1"
                     /translation="MSVTPENNEKVYVCRCEEVTLEEIDEWIDRGYDTVEELKRVLRV
                     GMGPCQGRGCQDILMREIAKKTGKPISEVAPATVRPPVKPIKIGLLAEGGEE"
     gene            5964..>6653
                     /locus_tag="PK849_04900"
     CDS             5964..>6653
                     /locus_tag="PK849_04900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPE65496.1"
                     /translation="MEWKSTAEVVIVGGGIIGVSTAYYLAKQGVKDIVLVEKGTICGG
                     STGRCGAGIRAQWGTEMNCRLGIAALDIFEQLDQELGMSVGLNQGGYLLVAYNEKELE
                     TLRNGMLLQNSLGIQSRMVTLTEAREICPGLAAEDAVGFFFHGRDGHADPFLTTFAYL
                     EAAKRLGVTCYRNTACTGVIVRDNTVQGIETTNGKISAPIVINCAGAYAQIVARMAGI
                     ELPNWAERHEIM"
BASE COUNT         1827 a         1633 c         1714 g         1479 t
ORIGIN      
        1 cgcgcctgcg cggttttgcg ccggggcgat tcagtttcaa cgtcaagggc ggaagatgcg
       61 aagcatgcgg cggcggcggc gtcaccaaag tttcaatgct cttcctcccc gacgtctacg
      121 tgccgtgtga agtgtgcggg gggaagagat ataatcacga gacccttgaa gtaaaattca
      181 aaggaaaatc cattgccgat gtccttgata tgactgtaga tgaagcgtat gctttctttc
      241 gtgatattcc acggatcgct tccaaacttc agttgatcgc agaggccggc ttggggtata
      301 tccgccttgg ccagtccgca ctcactctga gcggaggaga agcgcagaga gtcaaactct
      361 ccaaagagtt gagcaaacgc ttcagcggcc ctacacttta cctcctcgac gaaccgacta
      421 caggtctctt ttacacagat gttgagaaac tgctccacat catcaataaa atagtcgaca
      481 acggcaacac tgtcattttg atcgagcata acctggacgt tctgctctcc tccgactaca
      541 tcgttgacct gggaccggaa ggggggagcg gaggaggccg tctcgtcgca accggaaccc
      601 ctgaagatat catgacggga gagaagggac ataccgcata cttcctgcaa gaatatgcga
      661 agcaaatcgc cgaaagggtg aaaaagagtg gaaaaaagaa tggaccgaac tccggacaaa
      721 agagttcgaa gcgcgccgaa aaacgaggag aaacggaatt atcgcaaatc agttccaccg
      781 agaggagaac gaagcgaaga gaagcgaagt gaggatattc aatggggaag gcaggtcgtc
      841 ctccagcttt tgaaggaatc gcctgacaaa gtgcagaagg tctatctcgg taaaaatgtg
      901 agcgaccgtt tttcgctcca gataggcgat ttggcagaaa aagggaaagt caccgtcttg
      961 agagtaacgc ctgaagcgct caccgatatg tgcggaacca ataatcatca gggggtggcc
     1021 tgcagaagcg gagatgtatc gatgcttgag ctgcaggata ttcttcctct tcttgacggg
     1081 aagaaagggc ctgtacttct tgtgctcctt gatcacgtcc aggatcccca caacctcggg
     1141 gctgttgtgc gtactgccga ggcttgcggg gcaatggcag tcatcttccc taaaagacgc
     1201 gccgcccttc caggagggac tgtcgtcaaa gtaagcgccg gagcggcatt acgcgttccc
     1261 atggtggcag taacgaatgt tgcacaaaca gtcagagaga tccaacagaa cggattctgg
     1321 actatagggc tggataataa gaacggccgc tcgctctggt cggagaccat gccggaaaag
     1381 accctgcttg tagtaggcgc ggagggggag ggactctccc gtcttgtggc ggaaacgtgc
     1441 gatgagaccg tccggatacc ggttgccggt gcaacaggtt cactgaacgc aagcgtcgct
     1501 tgcgccgtcg gaatgtttga atgggcgagg aagtgggggg cgcaatccta gttttgctgt
     1561 aaaaagtttt cagcttcaac aaacaacgcg caggttcaag gattactccc tgaatcggcg
     1621 cgttgaaaca aaagctaatt tgcttttaat ctatggatcc ttaattgatg atttgagatt
     1681 cttcttttac tccagctctc agaaagaaaa aaagggaagt cccgtcgaca ggcgccgcac
     1741 ctcacttctt aaatccgttg gagtagcgat ggcaacaaca ggaatcttcg tgcatcaaac
     1801 tggaggatct tgaaaaatgc aaatcaggag agaagccttc ggcaatggca taatctacag
     1861 cgcaatacaa ttttccttcc tccaccatac cgtttcgctt ccattcgtcg gccatcggac
     1921 aaaaagagac atcgcttcca ttttcctgct tcaatgcgtc ccatgcatac gcaagaggca
     1981 aatcgtagtg ttcattcatt cctttcaaat ccagcgcatc acccgcagcg agcgcttgtt
     2041 ccctgacacg ccgcccacgt tcaagaccga actgacgcac aacattctca acaagagcgg
     2101 tccctttctc ctgccccagc atccctaccg cctcacgggc aaaaatgcca tggtagagag
     2161 ccaatgcacg ggccattgca tcgacgctct ttctcaatag agtttcatct tcttctctca
     2221 tatcctcccc accttctttc cgagtgtttc cccggatgcc tgcttatatt cccaagtcta
     2281 tagcctttcc attctctacg ccccgctgga aaagaaaccg cgctacgaaa agatcgaaag
     2341 cagcatagcc gacggatttg aaaaacgctc cggactcagc ggtaaagtcg gctttttcac
     2401 gggatagaaa agctcccatc gaacggatat ttttctttga aacaacacct gactgtaaag
     2461 gttcaataag gtcgccagtc tcgtcgaaag catgctcagt atctacaaaa atgtttgata
     2521 ttgctctgaa aagcgcgtcc ggatactctc tcatatccgg acggtacgaa cctataccga
     2581 caaaagtttt cccttcgaaa tacatcggac tatcctcaaa aacaggagtc gtggtattgg
     2641 tcgcagttat taccacatcc gagtgcttta acaaaaatgt tgtatctttc gtaatctcga
     2701 tctccaattc ggggaggact ttacgtatac gttcggcaca aaactcggct ttcgattcga
     2761 ttgtatcata aagaatcact tgggaaaaag gacgaacgga acacgcaaag cggacctgcc
     2821 aatagctttg ttccccagcg ccaacgattc ccaagcgggt tgcatgagta gaagcgaggg
     2881 tgcgaacagc caagcctcct acccctccag tccgaatcgc ggttaaagtc cttccgtcaa
     2941 gaagagcgga gggactgcca tccgaaccgt cgaaaagaag aaaaagaccg ttaataaatg
     3001 gtttgagtcg taccggatta tcgggaaaga gtgtcaacac cttcagtccc cactctttct
     3061 cagaaagaca gggcatcatc atcagtgtat ttttctcaag agagacagaa ttacggagag
     3121 gagcgataag gctgtttggg gatgtttcaa tctgaatcat ggcattggca acagcgtcca
     3181 ttacacctga gaaagaaaaa gaagaaatca tgctttccgc attcaagtaa tacataacat
     3241 acaaccccct ttgttagaat aaaagaagga aaaatgaata taaaaagaag cgaaatggca
     3301 atataaagta caggatatat tcaaaacacc ccttaagcaa cccccaatta tttttatctt
     3361 ttttatctat ttttacttta tcgatataaa agataaatac atgttctgcc tattttgcct
     3421 ctttcaatag cttcggaaaa aaatcacgaa ttgactcttg acaaagttcc acctcacgga
     3481 agagaattga acacagtaaa ccctatccta attcgggagg agatatcaac ccgagctaaa
     3541 aacacgttat acgtccatcg tcttcaccgc tctatatttt tccagatttc gcgcataacg
     3601 gcggacacaa aacagatacc gccctttagg ggggtattac gccatgtgat ttgtgcatgt
     3661 ttttgatctg cgaaaggagg aagctctttt ggatgagaaa gtaggcccgg gaggaaaagg
     3721 aagcaaactc gccctgatcg aggaacatcc tattcttgat tttgtccacg gggaaaaggt
     3781 ccgcttcttt tttgacggta aggagctcga aggatttgag ggagaaccta tttcaatggc
     3841 gcttcatgcc aacggagtgc gaatctaccg ggaaacggcg gaaatgcaca ggcctagggg
     3901 atttttctgc gcaataggta aatgcagcag ctgcttcatg attgtcgacg gggttcccaa
     3961 cgtgcgaacc tgcattacgc cactgaaagc cggaatgaaa gtcgaaaccc agcatggtaa
     4021 agggaacgtt cccgacttaa acgacgggag gccgcgttca tgaaaaaaat agccccagaa
     4081 atcctcgtaa tcggtggagg cgccgcagga ctgagcgcag cggcagaagc cgcatcggcc
     4141 ggagcgaagg tattagtcgt cgaaagcgat cttcgcctcg ggggccagct catcaaacag
     4201 actcacaagt tcttcggcag taaggatgaa tatgccggaa cccgcggtta caaaattgca
     4261 gatatcctgc tcaacgaaat cgattccttc aaagacagcg tttctatcct tcaaaacacg
     4321 acagtcacag gttattacac cgaagacgac accttcacgg ccatgcgggg cgaggaggaa
     4381 gtttttcaga taaaaccata aaaaaccatt gttgcaactg gagcgcaaga acggcttatt
     4441 cccttcccga acaacgatct tcccggcgtt tacggagccg gcgcggtaca gactctcatg
     4501 aatgtttacg gtgttctgcc cggaaaacga gtcctcatgg ttggagcagg aaacatcggc
     4561 cttatagtta gttatcaact gatgcaggca ggcgttgaag tcgccgccat cgtcgaagcc
     4621 ggtccgcgta tcggaggcta ttgggtccac gcggcaaaaa tacggcgcat gggcgtccct
     4681 attctgctgc gccattcaat aaagaaggcg ttgggagagg acgttatcac cggcgcggtt
     4741 attacggggc tcaatgaaaa attcgaacaa gtgggccctg aagaagaaat agagtgcgac
     4801 ttgatttgca tcgctgtcgg attgagccct accacagagc ttctttcgca ggccggatgt
     4861 aaaatgatgt acgttccgca gctttgcgga aatgttgcac aaagagaccg cacaatgaga
     4921 acctcgaacc cggcttttta tactgccgga gacgccagcg gaatcgaaga agccagcgca
     4981 gctatggttg aaggacgcat tgcaggtctt tcagcagcag cagctcttgg ttatcctacc
     5041 gagcatggca aaatcgacga atactggagc cgtcttgaat cgctccgaat gggcgaagct
     5101 ggagcccgga tatgtcaggg tatcgaatgc gttctcgtaa aaggctggga gggtgcatct
     5161 catgagtgac aaaaacatgg atatgctatt caacagcgga gtcttgaccg gagaaagaga
     5221 aggggccatc cttcctccac gtgaactctg ggagaagaaa aagggcggtg tcgtgataat
     5281 tgaatgccct cagagaatcc cctgtaatcc ctgccataca agctgtccta cgggagctat
     5341 tcttccgttt gaggatatca ataatactcc gaaagttaac tgggaaaagt gcacgggttg
     5401 cactctctgc gtctcagcct gtcccggact cgcatgcttc gttattgatc ttacgttctc
     5461 agaggagaat gcactgtaca agctgccgta cgaaatgctg ccccttccgg taaagggaca
     5521 gaaagtccag tgccttgacc gagaaggaaa gtccgtcggc gaaggaaccg tcgagaacgt
     5581 tactcaaccg cgcaaggata ggacgcacgt cgttcatgtc tctgcgccga agagccttgt
     5641 aacgattttt cgttcgataa gggtggtgaa ataatgagcg ttacgcctga gaataacgaa
     5701 aaggtctatg tctgccgatg tgaagaggtc acgctcgaag agatcgacga gtggattgat
     5761 cgcggatacg acaccgtcga ggaattgaaa cgcgttcttc gagtcggaat gggcccatgc
     5821 cagggacgcg ggtgccagga tatactcatg agggagatcg cgaaaaaaac aggaaagccc
     5881 atttcagaag tagccccggc aacggttcgc ccccccgtaa aacctattaa gatagggctc
     5941 cttgcagaag gaggtgagga gtgatggagt ggaaatcaac cgctgaggtt gtaatcgtag
     6001 gcggaggtat catcggagtt tccaccgcct actatcttgc gaaacaaggg gttaaagaca
     6061 tcgtcctcgt tgaaaaggga actatctgcg gaggttctac gggacgttgc ggcgcaggaa
     6121 tccgtgcgca gtggggaacc gaaatgaact gccgtctggg tattgccgct ctggacatat
     6181 tcgagcagct cgaccaggaa cttgggatgt ccgtcggatt gaatcaggga gggtatctcc
     6241 ttgttgcgta taacgaaaag gagctggaaa cgctccgtaa tgggatgctc ctccagaact
     6301 cacttggaat acagtcgcgc atggtcactc tcaccgaagc ccgcgaaatc tgtccgggcc
     6361 tagcagcgga agacgcggtc ggtttctttt tccacggcag agacgggcat gccgacccgt
     6421 tccttacgac attcgcttac cttgaagctg ccaagcgcct cggcgtaaca tgctatagaa
     6481 acactgcatg cactggagtc atcgtccggg acaacactgt ccaggggatc gaaaccacta
     6541 acggaaaaat tagcgccccg atcgtcatca actgcgccgg agcatatgct cagatagtcg
     6601 cccgtatggc aggtatcgag cttcccaact gggccgagcg ccatgagata atg
//
LOCUS       DAOPKU010000113         6652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12269_length_6652_cov_4.636198, whole genome shotgun sequence.
ACCESSION   DAOPKU010000113 DAOPKU010000000
VERSION     DAOPKU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12269_length_6652_cov_4.636198"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..670)
                     /locus_tag="PK849_04905"
     CDS             complement(116..670)
                     /locus_tag="PK849_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPE65497.1"
                     /translation="MKSTDELMDEHRGIEVMLDVLDAVARKVATGKPADLGHLDGMME
                     FLSVFADKCHHGKEEEFLFPSLEAAGVPNEGGPIGVMLSEHRKGRECIAGMKQGVAAL
                     KSGEKKGIALFADNAAAYTELLRQHIQKEDNVLFVMAGRCLSDAQDEELFREFEKLEE
                     ERIGKGRHEQFHALLDDLQKIYLG"
     gene            complement(1062..1487)
                     /locus_tag="PK849_04910"
     CDS             complement(1062..1487)
                     /locus_tag="PK849_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HPE65498.1"
                     /translation="MKVLVSACTIGCNCKYNGKNNRNEAVVEYLRDKEVISICPEALG
                     NMGTPRPCAEIVDGVVMNENGKNVDAEFRNAVELALEQIKDEAIDLVILQSRSPTCGV
                     NRIYDGTFSGKLIPGQGLFAQALVQRGYTVVDAEDVSKL"
     gene            complement(1548..1889)
                     /locus_tag="PK849_04915"
     CDS             complement(1548..1889)
                     /locus_tag="PK849_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65499.1"
                     /translation="MLTQGGFHNVTLVADGKEAWERLAMKNERYDLLVTDVEMPRLDG
                     VALTKQVKEHPELKRMPVVVFSSIMVQDVRLKISGTGADAHVTKPELPRMVEKVCRLL
                     LDSKEKAAATK"
     gene            complement(1859..2452)
                     /locus_tag="PK849_04920"
     CDS             complement(1859..2452)
                     /locus_tag="PK849_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPE65500.1"
                     /translation="MEVQNNEWQVVVFFLGGQAFAISVDKTREILRWPGSRPIPESHP
                     AMIGITSVRGEVMPLLDLRAYLGITPKVPLESSKVIIAEFNESKLGFVVDGVERIYGI
                     SAAELDSTLTSAVLGKSILYVIKRNESNVLIPDYEAIIQAAAPSLDRSLSIDLKKVAE
                     LAAPLGDLDRYRILSQRTRRLSGRRYPMCSPRAASIT"
     gene            2694..3422
                     /locus_tag="PK849_04925"
     CDS             2694..3422
                     /locus_tag="PK849_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HPE65501.1"
                     /translation="MFNILETREKLLKKFVEQGNEPRILLKGAGYGIRVILQDDAAED
                     ELLEEARASVEELARIAGDTGLVIDCKMRKLTPDFLSRLLCDFVWAGGFRVLSWTSDD
                     TETLELFRNTGFTTGEPVSGRPDRERVESGSLILTQSLRSGQMVEHDGDVMLLGHMNE
                     GAEIRASGNIFVHGRLKGVVHAGTRGGEHGVWAERFEATQVRIGGKICSSLGRESAWW
                     GKAVLIALENGQLVARELKLDFAS"
     gene            complement(3518..4108)
                     /locus_tag="PK849_04930"
     CDS             complement(3518..4108)
                     /locus_tag="PK849_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPE65502.1"
                     /translation="MRSKAYVLVFDGLADWEPALAMCQIRKSGKLEIVSVGFSKENVV
                     TMGGFTICPDIALDEVNVDEAGLFLLPGGDMWEQGGSDAVRELLGRLSSNGVPVAAIC
                     GATLEVVRAGLTRGVRHTSNALGYLKAVLPGYADEEFYVDRPAVADGNLITAGGLDRI
                     EFAYEIVKRLEIFDEAEAGELYEMFKNGVVPARYMA"
     gene            complement(4230..4724)
                     /locus_tag="PK849_04935"
     CDS             complement(4230..4724)
                     /locus_tag="PK849_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HPE65503.1"
                     /translation="MKIPTTLKHKPVVAVENYENVDGRQAYNSEAKGLSLGLAQWNDR
                     GRVDVSAKVWRHTGGKWSRQSEELPMHRVIDMAILVCSAKRYFSESYRRPEPYDKENP
                     RIDRIGLQGDAMSVEICTGNPMIDQDIELFQEALRRDDELMAERLHTLANLLIGMGYG
                     NCAR"
     gene            complement(4726..5058)
                     /locus_tag="PK849_04940"
     CDS             complement(4726..5058)
                     /locus_tag="PK849_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPE65504.1"
                     /translation="MDRKKELLMEYRQRKREMGVFLFECAEGGRYIGRADDPKGAINK
                     NVFQLKMGSHPNKRLQKEWSDRGGAAFSVRVLDSLEYDEETKAECAEELAMLFDDWIQ
                     KMKDAEVV"
     gene            complement(5273..>6652)
                     /locus_tag="PK849_04945"
     CDS             complement(5273..>6652)
                     /locus_tag="PK849_04945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE65505.1"
                     /translation="NDGLWDWNLLTGKVYFDPRYYTMAGYEPNEFPSSFDEWAKRLHP
                     DDHPLCNRALQSYFSGKTPVFDMEFRFRRKDGSWMWIRGRGRRVESDANGNAVRMVGT
                     HIDITELKQQQQEIQYLSFHDHLTGLYNRRFFDEELARLDTPRNMPLTLFLMDVNGLK
                     LTNDAFGHQAGDRLLRTVADVLRRTCREDEIIARIGGDEFILLLPQTGEEDAASLGIR
                     LREAFENVRMETGTLSVAFGWATKNDMSQPAGNVFITAENRMYQQKIYEMNSVRSKAI
                     SFILETLYENIPRERRHSENVGVLSGRIGVAMDLDAEAVKELVLAGKLHDIGKIAIGA
                     EILDKNGSLNESEWLTVKRHPEIGYFILNSVSDYASLAIIALAHQERFDGRGYPKGLS
                     GGNIPLHARIIAVADAYDAMTSGRPYRPPLSSAEALREIRAASGTQFDPSVVEVFLEL
                     FKKSPESIC"
BASE COUNT         1274 a         2074 c         1886 g         1418 t
ORIGIN      
        1 caaagcggcg ctaccgggtc ggggcatcgg gtcggggcat cggttcgggg caagcgtgcc
       61 ggttcgttcg gcgcacgctc tgtcgccgca cgccggaggc gcagcgcggc ggaggtcagc
      121 cgagatagat tttttgcaga tcgtccagca gggcgtgaaa ctgctcgtgc ctcccctttc
      181 cgatccgctc ctcctccagt ttctcgaact cgcggaacag ctcttcatcc tgcgcatcgc
      241 tcagacaccg gccggccatg acgaagagca cgttgtcttc cttctggatg tgctgtctca
      301 gcagctccgt gtaggccgcc gcattgtcgg cgaagagcgc gattcccttc ttttcccctg
      361 atttcagcgc cgcgacgccc tgcttcatgc cggcgatgca ctcccgcccc ttgcggtgtt
      421 cactcagcat gacgccgatg ggccccccct cgttcggcac tcccgccgct tcaaggctcg
      481 ggaagaggaa ctcctcctcc ttcccgtggt ggcacttgtc ggcgaagacg gacaagaact
      541 ccatcattcc gtccaggtga cccaaatcag cgggttttcc ggtcgcgacc ttccgggcaa
      601 ccgcatcaag aacatccagc ataacctcga ttcccctgtg ttcgtccatc agttcatccg
      661 tcgacttcat agcgctctcc cctttcgcgg gacaaaaatt ggttcttcgt ttcgcacagc
      721 atatatacag tatagtcagt ctctttgttt ttttcaagag aacgccgcgt tccggccccc
      781 ggcgaaggat tccaaggaac gaagcatttg tgcaataata gagaacatcg ccgggagatt
      841 catgacgtat gattcggtac acgggaggcg cgacatggga atcgaattct atcgcgatgc
      901 gagtttgccg tttttcgagc tgaaactctg cgccgaggag gaagtgcact acaagcggca
      961 ttcgcacgag gagtgctccc ttggcatcct ccagcagggg gagacgctct cggcggttct
     1021 ttcgcgatca ccgctcgaac agcggggcgg ccaagaaggg cttacagttt cgacacatcc
     1081 tcggcgtcga cgaccgtgta ccccctctgt acgagcgcct gcgcgaagag cccctgtccg
     1141 gggatcaact tccctgaaaa tgttccgtcg tatatccggt tcactccgca cgtggggctt
     1201 cgtgattgca gaataaccag gtcgatcgct tcatccttta tttgttccaa ggcaagctcg
     1261 accgcgttcc gaaattcagc atccacattt ttgccgttct cgttcatcac cacgccgtcg
     1321 acgatttcag cgcacggacg gggcgtcccc atgtttccca acgcttcagg gcagatgctt
     1381 atgacttcct tatcccgcag atactcgacc accgcttcgt tcctgttgtt tttcccgtta
     1441 tatttacaat tgcaccctat cgtgcacgcg cttaccaaga ctttcatagt tcctccccat
     1501 aaaaacggag ggcgcgaacg ccctccgaaa cggtgttcag acagctctta cttcgtcgcc
     1561 gcagccttct ccttcgagtc cagcaggagg cgacagacct tctcgaccat acgcgggagt
     1621 tcgggcttgg tgacgtgtgc atcggcgccg gtaccggaga ttttgagccg gacgtcctgc
     1681 accatgatgg aggagaagac gacgaccggc atccttttca gttcggggtg ctccttcacc
     1741 tgcttggtga gggcgacgcc gtcgaggcgg ggcatttcga cgtcggtgac gaggagatcg
     1801 tagcgttcgt tcttcatcgc cagacgctcc caggcctctt tgccgtcggc gacgagcgtc
     1861 acgttatgga agccgccctg ggtgagcaca tcggatatct gcgtccggat aagcggcgag
     1921 tcctctgcga gaggatacgg taccggtcaa ggtctccgag aggcgcggcg agttccgcga
     1981 ccttcttcag gtcgatggag aggcttctgt cgagggaagg agcggccgcc tggatgatcg
     2041 cctcgtaatc ggggatcagg acgttgctct cgtttcgctt gatgacgtag aggatggact
     2101 tgccgagcac cgcgctcgtc aatgtggaat ccaactccgc ggcgctgatt ccgtaaatgc
     2161 gttcgacgcc gtcgacgacg aaccccagtt tactctcgtt gaactccgcg atgatcactt
     2221 tgctcgattc cagcgggact ttcggcgtga tgccgaggta cgccctgagg tcaagcaggg
     2281 gcattacctc tccgcggacg gatgtgatgc cgatcatggc ggggtgggac tccggaatag
     2341 gacggctgcc cggccagcga agaatttccc tggtcttgtc cacgctgatg gcgaacgcct
     2401 gccctcccaa aaagaagacc actacctgcc actcgttgtt ttgtacttcc atgaacgctc
     2461 cctcctctgg ccggaacgga atctctcagt tcattacaat atgatatgcc attcagcccc
     2521 ggaccacaag tcccgtgagc gaaggtcgcc cgttacgcgc cgctcccaca tttaaaaatt
     2581 cgctctggaa tcaaaagatt gcgctcctcc gcttgactct cacactatgt caaggtgtag
     2641 gctgtttctc aagagagaaa tcaccgggga gaacgcgaaa gggagtgaac gccatgttca
     2701 atatcctgga aaccagggaa aagctgttga agaagttcgt cgagcagggg aacgaaccgc
     2761 ggattctcct caagggagcc gggtacggca ttcgcgtcat attgcaggac gacgccgcgg
     2821 aggacgaact cctggaggag gcgcgcgcct ccgtcgaaga actggcccgc atcgccggcg
     2881 acaccggcct ggtgatcgac tgcaagatgc gcaaactcac gccggatttc ctttcccgac
     2941 tgctctgcga cttcgtatgg gccggaggtt tccgcgttct ttcatggacg tcggacgata
     3001 cggagacgct ggagctcttt cgcaatacgg ggttcaccac cggagagccg gtctccggac
     3061 gcccggacag agagcgcgtc gaatcgggct cgctcatcct gacgcaatcg cttcgctcgg
     3121 ggcagatggt ggagcacgac ggcgacgtca tgcttctggg gcacatgaac gagggcgcgg
     3181 aaatccgtgc gtccgggaac atcttcgtcc acggcaggct caagggcgta gtccacgcgg
     3241 gaacgcgcgg cggcgagcac ggcgtgtggg cggagcgctt cgaggcgacg caggtgcgca
     3301 tcggggggaa gatctgttcc tccctcggca gggagagcgc ctggtgggga aaggcagtgc
     3361 tgatcgcgct ggagaacggg cagctggtcg cacgggagct gaaactcgac ttcgcgtcat
     3421 aaaaaaaaga cgcccgcgcc cctgaaggcg ctctgcggcg tcgcggaact ccatgaaggg
     3481 gagagcgcaa cggccgccgc gctctccctt tcgcatgtta cgccatatac cttgccggga
     3541 cgacgccgtt tttgaacatc tcgtacagct cgcccgcctc ggcttcgtcg aagatttcca
     3601 gccgcttgac gatttcgtac gcgaactcga tcctgtcgag ccctcccgct gttatcagat
     3661 ttccgtcggc gaccgccggc cggtccacgt agaactcctc gtcggcgtat ccgggcagca
     3721 ctgccttgag gtagccgagg gcgttgctcg tatgccggac gccgcgcgtc agcccggccc
     3781 gaacgacctc cagcgtcgcc ccgcaaatcg cggcgacagg aacgccgttc gaggaaaggc
     3841 gtccgagcag ttcgcgcacc gcgtcgcttc ctccctgttc ccacatgtcg ccgccgggta
     3901 gaaggaaaag ccccgcttcg tcgacgttca cctcgtcgag cgcgatatcg gggcatatcg
     3961 tgaatccgcc catcgtcaca acattctctt tcgaaaagcc gacgctgaca atctccagct
     4021 ttcccgattt tcgtatctgg cacatcgcga gcgcaggctc ccagtccgcc agaccgtcga
     4081 agacaagtac gtacgctttg gagcgcataa aaaaacctcc ttcaattcct gaaaatacgt
     4141 caaatcgctc tcagttcacc acgaaaaaac ctacaagagc aagatgtttc cgccaattca
     4201 atcgaaactc ccgacctccc gaacactcct caccgcgcgc agttcccgta ccccatcccg
     4261 atcaggaggt tggcgagggt gtgcagccgc tcggccatca gctcgtcgtc gcgccggagc
     4321 gcctcctgaa agagctcgat gtcctggtcg atcatcgggt ttccggtaca gatctccacg
     4381 ctcatcgcgt ccccctgaag cccgatcctg tcgatacggg gattttcctt atcatacggc
     4441 tcggggcggc ggtagctctc cgaaaagtac cgcttcgccg agcagacgag gatggccatg
     4501 tcgatcaccc tgtgcatcgg cagctcctcg gactggcgcg accacttccc gcccgtatgc
     4561 cgccagacct tcgccgaaac gtccaccctg ccccggtcgt tccactgggc cagccccagc
     4621 gaaagcccct tggcctcgga attgtacgcc tggcgtccgt ccacgttttc gtagttctcc
     4681 accgcgacca ccggtttatg tttcaatgtc gtcgggattt tcatattata cgacctccgc
     4741 atccttcatt ttctgtatcc agtcgtcgaa gagcatcgca agctcttcgg cgcactccgc
     4801 tttcgtctcc tcgtcgtact ccaacgaatc cagcacgcgg acggagaacg ccgccccgcc
     4861 gcggtccgac cactccttct gcaggcgctt gttcgggtgc gaccccatct tcagctgaaa
     4921 gacgtttttg ttgatcgcgc ccttcgggtc gtcggcgcgc ccgatgtagc gtccgccctc
     4981 cgcgcattcg aaaagaaaga cgcccatctc ccgcttccgc tgcctgtact ccatcagcag
     5041 ctcttttttc ctgtccacga tacgacacct cgaatccaac gagtcattta ctaaattact
     5101 cgtttactaa ttatcacgga tgaatagtac cacatctgcc gaagacgtca agcgctccgg
     5161 cggaaaaaaa ccagtttttc agccgtgttg ctcgacgatc agtggaaaga tgcgggtggg
     5221 aaaaaaggga gggaaaggcg cgccccggcc gtgatgcaac cggaggcgcg tgtcaacaga
     5281 tggattccgg ggactttttg aagagctcca ggaacacctc gacgacgctc gggtcgaact
     5341 gtgttcccga cgcggcgcga atttcacgca acgcctcggc ggaagagagc ggagggcggt
     5401 aggggcggcc gctcgtcatc gcgtcgtacg cgtccgccac cgcgatgatg cgcgcatgga
     5461 gcggaatgtt cccgccgctc agtcctttcg gatagcccct tccgtcgaac cgctcctggt
     5521 gggccagcgc aatgatggcg agcgacgcgt agtcgctcac cgagttcagt atgaaatacc
     5581 cgatctcagg gtgcctcttt acggtcagcc actcgctttc gttcagggaa ccgtttttgt
     5641 cgaggatttc ggcgcctatg gctatcttgc ctatgtcatg aagctttcct gccagtacga
     5701 gctctttgac cgcctcggca tccaggtcca ttgcaacgcc gatccgcccg gagaggacgc
     5761 ccacgttctc cgagtgccgg cgctcccggg gaatattctc gtacagcgtc tccagaatga
     5821 aggagatggc tttgctgcgg acgctgttca tctcgtaaat tttctgctgg tacatcctgt
     5881 tttcagcggt gatgaagacg tttcccgcag gttgcgacat gtcgttcttg gttgcccagc
     5941 cgaatgcgac ggaaagagtc cccgtctcca tcctgacatt ctcaaaggcc tcccgcaatc
     6001 gtatgccgag cgacgcggca tcctcttcac cggtttgggg aagcagaagg atgaactcgt
     6061 cgcctcctat gcgggcgata atctcgtcct ccctgcaggt tcggcggagc acgtccgcca
     6121 cggtgcggag cagacggtcc cccgcctggt gaccgaaagc gtcgttcgtc agcttgagtc
     6181 cgttgacgtc catgagaaag agcgtcagcg gcatgtttct cggagtgtcc aggcgcgcaa
     6241 gctcttcgtc gaagaaacgc cggttgtaca gtccggtgag gtggtcgtga aagctgaggt
     6301 attgaatctc ctgctgctgt tgtttcagct cggtgatgtc gatgtgcgtc ccgaccatcc
     6361 ggacggcgtt gccgttcgcg tcggactcga cgcgccgtcc ccgtccgcgg atccacatcc
     6421 acgagccgtc cttccggcgg aagcggaact ccatatcgaa cacgggcgtc tttccggaga
     6481 aatacgactg aagagcgcgg ttacataatg gatggtcgtc cggatgcagc cgcttcgccc
     6541 actcgtcgaa cgaggagggg aactcgttcg gctcatagcc ggccatcgtg tagtaccgag
     6601 ggtcgaagta caccttgccc gtgagcaggt tccagtccca caggccgtcg tt
//
LOCUS       DAOPKU010000114         6643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12288_length_6643_cov_5.139344, whole genome shotgun sequence.
ACCESSION   DAOPKU010000114 DAOPKU010000000
VERSION     DAOPKU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12288_length_6643_cov_5.139344"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..606)
                     /locus_tag="PK849_04950"
     CDS             complement(118..606)
                     /locus_tag="PK849_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HPE65506.1"
                     /translation="MATIRVGIAESGVVLNPNSVSTLGLGSCVGVTLYDERRKIGGMV
                     HVMLPSTELAKGADFNRSKFADSAVPDLLQQMIRSGADQRRLVAKLAGGAQMFNLGGD
                     ADSLLKIGARNVEACKKALSLLRIPIVAEDTGGSWGRTVELFSESGVLEIRAIGKAKT
                     HI"
     gene            complement(593..1288)
                     /locus_tag="PK849_04955"
     CDS             complement(593..1288)
                     /locus_tag="PK849_04955"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HPE65507.1"
                     /translation="MTGERRESGNEGFRSPETGDALQRMSDFHLDVLREVSNIGAGNA
                     ATSLSTLLGKTVDMRVSRVFPMPFNEIIEYVGGAERMILSVFVRIEGGISGNMLFVMS
                     REDAQSLVRHMTGATETEEQGFSEMGLSVLHEVGNIMIGSYITALSDFLGFELSPSVP
                     SLAMDMAGAILAFSLSEIGRSGDTAIVIDGALSLDGGALSGNGAGHIFLLPDPESFNK
                     IFQALGVAGNGDH"
     gene            complement(1285..1719)
                     /locus_tag="PK849_04960"
     CDS             complement(1285..1719)
                     /locus_tag="PK849_04960"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HPE65508.1"
                     /translation="MNIQWSDELAIGIGIIDDQHKKLIERIASFSHAVDGGDLHKVEE
                     TVNYLVGYAIQHFGAEELIMIRNCYDQFKEHRDEHSWFIKQVFDVHTSLVQKEECTQR
                     QIEDLRDMLYNWTLDHIMVKDKRIAQTLNSFGGPVRQGGGQA"
     gene            1864..2814
                     /locus_tag="PK849_04965"
     CDS             1864..2814
                     /locus_tag="PK849_04965"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPE65509.1"
                     /translation="MMITVLYGGEGPEREVSLRSGAAVTSAFAERGRDVTGADLRRKE
                     DLFALLDEGNVEFAFIALHGGWGEDGTLQAALEMAGVPFSGSGHAACALAMDKTASKA
                     LFRWKGISVPKGIEVARGSGARDVLDDPRFHPLLERSGKLVVKPCCSGSTVGVSILSG
                     TDRLAEALEDAFRYDPRVLVEEYVSGRELTVTVYEKEGVPRCLPVIEIQPVTGFYDYS
                     SKYTPGASEYLVPAPLSEFVLRRVEAASLAAHAALGCAAYSRVDLRLDEEGFPSVLEV
                     NTVPGMTATSLVPKAAAAAGISFGEFLEEVVESSLAARRG"
     gene            complement(3245..4192)
                     /locus_tag="PK849_04970"
                     /pseudo
     CDS             complement(3245..4192)
                     /locus_tag="PK849_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
     gene            4287..5069
                     /locus_tag="PK849_04975"
     CDS             4287..5069
                     /locus_tag="PK849_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPE65510.1"
                     /translation="MIFLTGDTHGLLEIDRLGTANFPLGERLGRNDLVVILGDFGLLW
                     SDPPRAEERLALRRLEESPWTTLFVDGNHENFSMLDALPEEERFGAPVGVVSPHVLHL
                     RRGYVYTLAGKRCFVFGGARSVDRKGRTRGRSWWPEEIPSAEEYRRGLDSLEASEWSV
                     DWILTHTAPNHILQALDLHKYLHGDPVSAYLDEIFKEVSYQKWFCGHLHRNSRFAKDR
                     IHVLRENIVNGDTEETVSVERDRPPLRERIRAITRLRPLEGD"
     gene            5203..>6643
                     /locus_tag="PK849_04980"
     CDS             5203..>6643
                     /locus_tag="PK849_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HPE65511.1"
                     /translation="MKKTFFRSSHKRAASLADILVVVLLLAAGFFLGEWRVSKAELGM
                     RHNLVRRARLFAESVNPRAVKRLSGSESDRVSPDYRRFKEQSVSLLSAFPDCRFISLL
                     GRRADGSIFIYFDSEPEGSPDESPPGERYVPPDPVLLEAFDKGKSVVAGPFMDIWGSL
                     VAAWVPVADPDTGKPAFVAALDIDGGKWRSDARRAAYVPECAAVLLAALFIVWRILTL
                     RRALRRRTTYRWYHRYADALFVAATGLVLTFSILLVSSEVERSTREDTFHMLADSESG
                     KLNNSLKNLRDIGVDGAAAFFAHSDFVDFEEFKGYARHLEELPEVRGATWLPFVPHEE
                     REAFERLGRSLVTEDFVIRDYGEGGKRVPAPERPFYYPVLYRIPFEGSERILGADFAQ
                     VPSRKLAIDEAHSSRLPAATEAIPLLAAPGTLKGIVVFQPVYFRNDTNKFMGVLGMVV
                     ELNALLNRMRTEEAHVERAVLVGDLWRLYG"
BASE COUNT         1174 a         2028 c         2059 g         1382 t
ORIGIN      
        1 tctcgtgaac actgattaac atctgctcgg cagggaaagg cgcagcaata gcttgccaac
       61 gttctccgtg ccgagcagat gtttatcagt gttcacgaga tgcctccgaa tctttgttca
      121 aatatgtgtt tttgccttgc cgatagcgcg gatttcgagg actcccgact cggagaacag
      181 ttccacggtg cgtccccacg agccgcccgt atcctcggcg acaataggga tgcgaagaag
      241 agagagcgct tttttacagg cttccacgtt ccgggcgcct attttcagca aggagtccgc
      301 atcgccgccg aggttgaaca tctgcgcgcc gcccgccagc ttggccacga gccgtctctg
      361 gtccgcgccg gagcggatca tctgctgcag caggtcgggg acggcggaat cggcgaactt
      421 cgacctgttg aagtccgcgc ccttggcgag ttccgtggaa ggaagcatca catggaccat
      481 accgccgatt tttcttcttt cgtcgtagag ggtaacgccg acgcacgagc ccaagcccag
      541 ggtcgagacg ctgttcgggt tcagtacgac ccccgactcg gcgatgccca ccctaatggt
      601 cgccatttcc ggcgaccccc agagcctgga agattttatt gaacgattcc ggatcgggaa
      661 gcaggaagat atgccccgct ccgttcccgg agagcgcgcc gccgtccagc gagagcgcgc
      721 cgtcgatcac gattgccgtg tcgccgcttc gcccgatctc cgagaggctg aacgcgagaa
      781 ttgctcccgc catgtccatc gccagcgacg gaacggacgg gctcagctcg aagccgagga
      841 agtcggagag cgccgtaatg tacgagccga tcatgatgtt ccccacctcg tggaggacgg
      901 agagccccat ctcggagaag ccctgctctt cggtctcggt cgctcccgtc atgtgccgga
      961 cgagcgattg cgcgtcctcc cggctcataa cgaagagcat gtttccggag atgcccccct
     1021 cgatacgcac aaagacgctc agaatcatcc gctccgctcc gccgacgtac tcgataatct
     1081 cgttgaaggg catcgggaat acgcgcgaca cgcgcatatc gaccgtcttt cccaacagcg
     1141 tggagagaga tgtggcggcg tttccggcgc cgatgttgct gacctccctg agcacgtcga
     1201 ggtgaaagtc gctcatcctt tgcagcgcgt cccccgtttc gggcgagcga aacccttcgt
     1261 ttccgctttc ccgccgttcg cccgtcatgc ctggccccct ccctggcgca ccgggccgcc
     1321 gaaactgttg agtgtctgcg cgatgcgctt gtccttcacc atgatatgat caagggtcca
     1381 gttgtacagc atgtcgcgga ggtcttctat ctgtcgctgc gtacattctt ctttttgcac
     1441 caggctggtg tgcacgtcga aaacctgctt gatgaaccag ctgtgctcgt ctctgtgctc
     1501 tttgaactgg tcgtagcagt tgcggatcat gatcagctct tctgccccga agtgctgaat
     1561 cgcgtacccc acaagatagt tcaccgtctc ctcgactttg tgcaaatcgc cgccgtccac
     1621 ggcgtgcgaa aaagaggcga tgcgctcgat cagcttcttg tgctggtcgt cgatgatgcc
     1681 gatgccgatg gcgagttcgt cgctccactg tatgttcatg tgtattcctc ccgtcagggg
     1741 cgatagaaac ttggcgggaa aacacccgtc tgctgtattg taccagaaag aagagcggaa
     1801 gaagactggt ttgtgtataa taatgggcca gaatacggat attaaggaaa ggattgaatt
     1861 cccatgatga taaccgttct ctacggcgga gaaggccccg agagggaggt ctccctcaga
     1921 agcggcgcgg ccgtgacttc cgccttcgcg gagcgggggc gcgatgtgac aggtgcggat
     1981 cttcgccgca aggaggacct tttcgcgctt ctcgatgaag ggaacgtcga gttcgccttc
     2041 atcgccctcc acggcgggtg gggagaggac ggtacgctcc aggcggcgct ggagatggcc
     2101 ggggttccgt tctccggctc cggacacgcc gcctgcgccc ttgcgatgga caagacggcg
     2161 agcaaggcgc tctttcgctg gaagggcatc tccgtaccga aggggatcga ggtcgctcgt
     2221 ggaagcggcg cccgtgacgt gctggacgat cctcgtttcc accctcttct tgaacgctcg
     2281 gggaaactcg tggtcaagcc ctgctgttcg gggagcaccg tcggcgtctc catcctctcg
     2341 ggaaccgacc ggctcgccga agcgcttgaa gacgctttcc gctacgaccc gcgggtgctt
     2401 gtggaggagt atgtctccgg gagggagctg acggtgaccg tgtatgagaa agagggcgtt
     2461 ccccgttgcc tgccggtcat cgagattcag cccgtaactg gattctacga ctacagctcg
     2521 aagtacacgc cgggagcctc cgaatacctc gttccggcgc cgctgtcgga gttcgtcctc
     2581 cgcagggtgg aagcggcctc cctcgccgcg cacgcggcgc ttgggtgcgc ggcgtacagc
     2641 agggtcgatc tcaggctcga cgaggagggg ttcccctccg ttctcgaagt gaacaccgtc
     2701 cccgggatga ccgcgacgag ccttgttccc aaggccgcgg cggcggcggg gatctccttc
     2761 ggcgaatttc tcgaagaagt tgtcgaatcc tccctcgccg cccggcgcgg ctgagactca
     2821 gtagtccgtc acgtactgga actgcttgcg gaactcgtcc ggcggcatct tcgccgccac
     2881 cgcgaggcgt tcgaactcga tgccggggtt gaaatccttc ttctccagcc ggatcatgta
     2941 ctgcctggcg atcctgtact ggatggactg tgtgttcacc atgcggatac gggtcttctt
     3001 cgtgaccggg tcgatgatct ggtcgaacgg gatgggcacg atctggttgt tctgaatggt
     3061 gatgagcgcg ttcgtccctc cgtcgcggag gaattcgaac gccccgtacc caaggttccg
     3121 cgtgtactcg atgtcgaagg cgttcggagg cgcgcagcgg acctcgtatc cgacttcttt
     3181 gttgttgatc gagtacacgc ggaaccttgg gtacggggag gttccgcgtg tactcgatgt
     3241 cgaaggcgtt gggaggcgcg cagcggacct cgtatccgac ttctttgttg ttgaccgtca
     3301 tcttcaggcc catcccggag agcttgacga gcagctcttt cttgaggacg tccgagaagt
     3361 tgatctcggc gtagcggatg tgcccgtggt cgtcgtactc caggcagtcc gcggcctcaa
     3421 gatcctccag gcggatcttc tcgatcagcc cctcggcggt gaccgcgacg ccgtagttcc
     3481 gccccgaggc caaccgtttc acaatggagc ccgtgacgat gtccaccacg agggagagcg
     3541 ggatcttctc cttccccggg aattcctcgg ggatgagcgt caacgtcgcc cccgcgctct
     3601 tgccgatgcc gagggcgaga tggcccgcca cgcgccccat cgcgaccacc aggtaccagc
     3661 gccccgtcgt cttggcgtcc tccatcaggt tggagacgat ctgcgtccca agcgaacgcg
     3721 ccgtctcgaa gccgaaggtg gggatgccct cggggagcgg aaggtcgttg tcgatcgtct
     3781 tggggacgtg aacgaagttg atggtcagcc cgaggctgtt cttcgcgaag tcggcgacgc
     3841 gcgaggccgc gtatgcggtg tcgtcgcctc cgatggtcac gaggtgcgtg actcccagct
     3901 cgatgagcgt gtccaccacc ttccgcagcg actcgtcgct cttcgtgggg ttgaaccggg
     3961 agatccgcag gatgctccct ccgtcggagt ggattcggct gacggcgtcg atggtgagag
     4021 ggatcacctt cttctccccc ttccccaggt tggagaagcc gtcgtagatg ccgtacactt
     4081 cccacccgga cttctttgct tctatggtga cggagctgat cacgctgttg atccccgggg
     4141 cgggacctcc tccgcagacg atggcgagtg ttttttgatc cggattcgtc atgaggcgcc
     4201 tccttcaata tgaaagataa aaagaaagac ttcgtaaaat tttagcacat cgcacggtat
     4261 aataaacggc gaaggagcgg atgcgcatga tttttcttac aggcgacacg cacggccttc
     4321 tcgagatcga tcggctcggg acggcgaatt tccccttggg agagaggctg gggcggaacg
     4381 acctggtcgt catcctgggc gacttcggcc ttctgtggag cgaccctccc cgcgcggaag
     4441 agaggctcgc cttgcgccgc cttgaggaga gcccctggac cacccttttc gtggacggaa
     4501 accacgaaaa cttctccatg ctcgacgccc tccccgagga ggagcggttc ggcgcgccgg
     4561 tcggcgtcgt ctcgccgcac gttctccatc tgcgccgcgg gtacgtctac acgctcgcgg
     4621 ggaagcgctg cttcgtcttc ggcggcgcgc gcagcgtgga caggaagggg cggacgaggg
     4681 ggagatcctg gtggcccgag gagattccgt cggccgaaga gtaccgccgg gggctcgact
     4741 cgctggaggc ttcggagtgg agcgtcgact ggattcttac ccacacggct cccaaccata
     4801 ttctgcaggc gctcgaccta cacaagtacc tgcacggaga ccccgtctcg gcctacctgg
     4861 acgagatttt caaagaagtg tcctaccaaa aatggttctg cggccacctg caccgcaaca
     4921 gccgtttcgc gaaagaccgt atccatgtgc tccgggagaa catcgtgaac ggcgatacgg
     4981 aagagaccgt ctcggtggaa cgcgatcgcc ctcctctcag ggaacggatc cgcgccataa
     5041 cgcgcctccg ccctctcgaa ggagactgaa cggctccgtc ttaaaaagta gcgaacggtc
     5101 gggcggagaa tagagtatac ttagaggaat cccgtgaaac tctagcgggg cctacggccc
     5161 cgttgccgcg ggacatgaag agccggaatc ccggaggcgc ctatgaagaa gacctttttc
     5221 cgttcatcgc ataaacgcgc cgcgagtctc gccgatattc ttgtcgtcgt gctcttgctg
     5281 gcggctggat ttttcctcgg cgagtggcgc gtgtcgaagg cagagctggg gatgcgccat
     5341 aacctggtcc gccgagcgcg ccttttcgcc gaaagcgtca atcctcgcgc ggtcaagcgc
     5401 ctctcaggtt ccgaatcgga ccgcgtttct ccggactacc gccgcttcaa agaacagagc
     5461 gtttcgctcc tctccgcctt tcccgactgc cgtttcatct cgctccttgg ccgccgtgcc
     5521 gacgggtcga tcttcattta cttcgattcc gagcccgaag gctcgccgga cgagtcgccc
     5581 cccggagaga ggtatgttcc tccggatccc gtactgctgg aggccttcga caaggggaag
     5641 agcgttgtcg ccggtccctt tatggatatt tggggaagcc tcgtcgcggc gtgggttccc
     5701 gtcgccgacc cggataccgg gaagcccgcg ttcgtcgccg cgctcgatat cgacggcggg
     5761 aagtggcgga gcgacgcccg acgcgccgca tacgttccgg aatgcgcggc ggtccttctg
     5821 gccgctctct tcatcgtatg gcggatactg acgctgcgcc gcgccctgag gaggagaact
     5881 acctaccgtt ggtatcaccg ctacgccgac gctctcttcg tcgccgcgac cgggctcgtg
     5941 ctgacatttt cgatacttct cgtctcatcg gaggttgagc gctcgacgcg tgaggatacc
     6001 tttcacatgc ttgccgactc ggagtcgggg aagttgaaca acagcctgaa gaatctgagg
     6061 gacatcggcg tcgatggggc ggcggcgttc ttcgcgcaca gcgatttcgt cgacttcgag
     6121 gagttcaagg ggtacgcccg tcatctggag gagctgcccg aggtgcgggg cgccacgtgg
     6181 cttccgttcg tcccgcatga agagcgcgag gcgttcgaac gtctcggacg ctcgctcgtt
     6241 acagaggatt tcgtcatccg cgattacggg gaggggggca agagagttcc cgctccggag
     6301 cgcccgttct actatccggt gctgtaccgc attcccttcg aggggagcga gcgcatactg
     6361 ggggccgact tcgctcaggt tccctccaga aaactcgcga tcgacgaggc gcattcctcc
     6421 cgccttcccg cggcgacgga ggcgattccc ctgctcgccg cccccggaac gctgaaggga
     6481 atcgtcgtct tccagccggt gtattttcgc aacgatacga ataagttcat gggagttctc
     6541 ggtatggttg tcgagctgaa cgctcttttg aaccggatgc gtaccgagga ggcgcacgtg
     6601 gagcgcgccg tgctcgtcgg cgatttgtgg cgcctgtatg gcg
//
LOCUS       DAOPKU010000115         6627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12334_length_6627_cov_5.240870, whole genome shotgun sequence.
ACCESSION   DAOPKU010000115 DAOPKU010000000
VERSION     DAOPKU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12334_length_6627_cov_5.240870"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK849_04985"
     CDS             <1..233
                     /locus_tag="PK849_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65512.1"
                     /translation="LHLEDAAQLKEDLIASVEKTVLSLGAKSVSDIDLLETKIKGRIR
                     DTLRRFGRSKPMITPLITVLGAPEKNGKGKRK"
     gene            230..1051
                     /locus_tag="PK849_04990"
     CDS             230..1051
                     /locus_tag="PK849_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPE65513.1"
                     /translation="MSEGLPAFFLGLLQGVTEFLPVSSSGHLALAKQLFSVEHASLTY
                     DVLLHFATMLATLLYFGKDILLLLREWCSGFFSPAARCEEGWTLGWAVLAGTVVTAAI
                     ALPLKPVVERMMELPGAVGAGLLLTSLLLWRGSCIASSLKGAGKPISLGRGFLIGLVQ
                     GVAVLPGVSRSGSTIVAGMSAGLSAPAAFRFSFLLSLPAVFGATLLEVRGLLKTAGWA
                     SSLPAGWIAGFFASFLAGFFSLVLLRRVVSAGKWRPFAVYCAFAGTLSIVLSLLG"
     gene            1057..1299
                     /locus_tag="PK849_04995"
     CDS             1057..1299
                     /locus_tag="PK849_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65514.1"
                     /translation="MASGSSVKWWIIVASLIIGTISGLYLQRFALTEPFFKDILSTGF
                     SLTDVHLGFADFGLKVWLRWNLGTIIGGVVGIWVAR"
     gene            1284..1880
                     /locus_tag="PK849_05000"
     CDS             1284..1880
                     /locus_tag="PK849_05000"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPE65515.1"
                     /translation="MGGSVNLVLASGSPRRRELLHSLGWSFRIDVPDICEDLLPGEGP
                     EEAARRLSRAKAAEAAARNPGALVVAADTLVAAGGVILGKPSCREESLRMLRMLSGAV
                     HSVFTGVALRRGEEERFAVEETRVEFRPLSEESMKLYVESGEGDDKAGAYAIQGRGAL
                     LVSSLHGDYFNVVGLPLCRLSRLLEEFGCSLADQWRNS"
     gene            1880..3853
                     /locus_tag="PK849_05005"
     CDS             1880..3853
                     /locus_tag="PK849_05005"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HPE65516.1"
                     /translation="MIKSTADFLETSRRNPAIFVALVLILLSAWALLPRLAAERESKD
                     AAIVLDYRDLATFSIREGKSPEMLWPLLSQNGASGLMVSELTGQQLSMGTLPLYYGPA
                     SGLPGAAVKLASGNPLRAAFFIPRTHPGAALLPPLLSARFRGAHVQEYETGTAVLLTR
                     SLEELFFTGVLPDIEGLLIAAKHDIPVFYRVAPALPSDTDASLATLGALLAAFPQIRT
                     VSPSGEVALGYPNLRPLAERIVKEGRSVAMVEFSRQIGAPQLNWLAYPRLVSLHSVTN
                     EEILSRNISRSALYERFVRAVKERAVRLLVFRPSAMEASDAPFERFIEELRTLASGIT
                     ASGAQLRWPEPFQPWRTGFVGALALALAFVFSMLRFLQRFFNGGTDDRKNAEDALAWR
                     TTPRGLAVFSAIVLLAGVIILLLPSAGRFAGALTAVFVVVEASFLALDGWRTPWLGII
                     GSFLFAVAGGLAIAAFFSDPASMLRLRSFSGVKATLFLPPLLVLLADLKRRVHPESLG
                     ELFRRPPLWGEIFLLVLLLGGAGLVLFRSDNVQFVPAFEVRMREFLERALVARPRNKE
                     VFLGYPALLLWYFVRKADMWPRYREVFRLATVIGFSSAVNSFCHFHTPLFFILFRQFN
                     GLWTGVLLGMISIVFLRFVIMPLWYRYGGLVTE"
     gene            3850..4887
                     /gene="csaB"
                     /locus_tag="PK849_05010"
     CDS             3850..4887
                     /gene="csaB"
                     /locus_tag="PK849_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HPE65517.1"
                     /translation="MRPEREFRVALCGYYGFGNLGDELLAEALIAALERCGIKRGEMA
                     MLSASPEESARAHGVCSVSRWSPRAVFRILRRSETFLLGGGGLFQDVTSLRSPLYYWG
                     ALRLALLAGARPWCAGQSVGPLLTKRGRLLARSALGSCSVRGVRDARSAELLADWGMN
                     SVVTCDPVFSLTPPVTSPRHGSALLVNIRPWTGGLASKTAEESARLASSRSLSLTGFA
                     LAAEDAGIMEELRRSGVFPAERIVLLDAENWRSECGRLFPEADGVVAMRFHASLLALL
                     FGRPLTAVAYDPKVESLASEWSLPSWSGSGALPEPAAPLGPFRPFEKGAEFLKTFSEM
                     CAAAMRRDGKE"
     gene            4892..5725
                     /locus_tag="PK849_05015"
     CDS             4892..5725
                     /locus_tag="PK849_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPE65518.1"
                     /translation="MLREPSFLAEKASEIRRDVVRMIGLARSGRLASSLSIVDILAWL
                     YWDVLSVDPADPWFESRDRFVLSKGRGAPALYATLANRKFFERDELWSYRRLGAMLQG
                     YPQALRIPGVDAPGGGSFGMGLGIANGLAIALRRGRSPCRVFCLAGEEELQEGAFWEA
                     VIAASRFCLDSICLVIDRYRTKEEAGIPSASDEAFCEKIRSFGWTAEACDGHDFSSLG
                     GALRLLGQGAGRPKCLLARTRLGKGVSFLEGDSSGESEVLDRERVDKALRELEKEEDP
                     L"
     gene            5722..>6627
                     /locus_tag="PK849_05020"
     CDS             5722..>6627
                     /locus_tag="PK849_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPE65519.1"
                     /translation="MNAAGRSTGDAYGHALISIAASDERIVALEGGDAAWSSPFSESY
                     PERCFNAGGAEQNLILTAAGLALGGDNVFVSSYAAFLTGKGYDQIRTAVAIPSLPVRI
                     AGLGAGLTAGEEGACHQTLEDIALMRVFPGMTVLVPADYTAAFALLKNVAGVQKPVYI
                     RLGAAATPDVYEEGDEAFFPGGGRVLREGTGVTLCCCGIMVHEALRAADVLARQNISA
                     EVIDCYSVSPLPVQAILDSVHRTGCCVTAEEHFASGGLGEAVASLTGANYPVPVKMVA
                     VFDKFGQSGSPPELKEYYGLTASQIV"
BASE COUNT         1029 a         1900 c         2141 g         1557 t
ORIGIN      
        1 tcctgcacct tgaagacgcg gcgcagttga aggaggacct gattgcctcg gtggagaaga
       61 cggtgctctc tctcggcgcg aagtctgtct ccgatatcga tctcctggag acaaaaataa
      121 aggggcggat acgcgacacg cttcgccgct tcggacgaag taaaccgatg ataacacctc
      181 ttataacggt tcttggcgct ccggaaaaga acgggaaggg gaagagaaaa tgagcgaggg
      241 tctgcccgcc tttttcctgg gactgttgca gggggttacg gagttcctcc ccgtgagcag
      301 ctccggacac cttgcgttgg cgaaacagct cttctccgtg gagcacgctt ccctgaccta
      361 cgatgtgctg ctgcacttcg ctaccatgct cgcgacgctg ctctacttcg ggaaggacat
      421 tcttctcctg ctccgtgagt ggtgttccgg attcttctcc ccggcagcgc gctgcgaaga
      481 ggggtggacg ctcggctggg ccgtacttgc ggggaccgtt gtaacggcgg ccattgctct
      541 tccgctcaag cccgttgtcg agcggatgat ggagcttccc ggagcggtcg gcgcgggcct
      601 tcttctcacg tcgctgctgt tgtggcgagg ttcttgtatc gcgtcctcgc tgaagggcgc
      661 cgggaagccg atttctctcg gccgggggtt cctgatcggc cttgtgcagg gagtcgcggt
      721 gcttccggga gtttcccgtt ccggatcgac gattgtcgcg ggtatgagcg caggcctgtc
      781 ggcgcctgcg gcgttccgtt tctcctttct tctttccctg cccgccgtgt tcggagcgac
      841 gcttcttgag gtgcgaggac tcctgaaaac cgccggctgg gcctcttcgc tgccggcagg
      901 ttggatcgcc ggttttttcg cttcgtttct tgccggtttt ttctcgcttg ttcttctccg
      961 gcgcgtggta tcggcgggga agtggcgccc gttcgcggtg tactgcgcat tcgcagggac
     1021 gctttcgatc gtcctttcgc tgctggggtg aatgcgatgg cgtccggctc ttccgtgaag
     1081 tggtggatca tcgtcgcttc gctcatcata ggaactattt caggactcta tcttcaacga
     1141 tttgccttga cggagccctt tttcaaggat attctttcaa ccggcttcag tctgacggac
     1201 gtgcacctcg ggtttgccga cttcggcctg aaagtctggc ttcgatggaa tttgggaacg
     1261 attattggag gcgtggtggg aatttgggtg gctcggtaaa tcttgtgctt gcgtcgggga
     1321 gccccaggcg gcgtgagttg cttcattccc tggggtggtc tttccggata gacgtcccgg
     1381 atatttgcga ggatctgctt cccggagaag gtcccgagga ggccgcgagg cgtctttcgc
     1441 gggcaaaggc ggccgaggcg gcggcacgaa atccgggggc gcttgtagtg gctgccgaca
     1501 cgctggttgc cgcgggcggc gttatcctcg gcaaaccgtc ctgccgggag gaatcgcttc
     1561 ggatgcttcg gatgctcagc ggggcggttc atagcgtctt caccggagtc gcacttcgcc
     1621 gtggagaaga ggagcggttc gctgtcgagg agaccagggt ggagttccgg cctctttcgg
     1681 aggagtcgat gaaactctac gtcgaaagcg gcgaggggga tgacaaggcg ggcgcttacg
     1741 cgatccaggg gcggggcgcg ttgttggtgt cctcgttgca cggcgactac ttcaacgtgg
     1801 ttgggctgcc gctttgcaga ctgagccgtt tgcttgaaga attcggctgc tctctggccg
     1861 atcaatggag gaactcgtga tgatcaaatc cacagctgat tttcttgaaa cttcgcggcg
     1921 gaatcccgcg atttttgtcg ctcttgtact cattctcctc tccgcctggg ctctcctgcc
     1981 ccggctggcg gcggaacggg agtcgaaaga cgccgccatc gttctcgact atcgcgacct
     2041 ggcaaccttc tcgatcagag agggaaagag cccggagatg ctctggccgc ttctttcgca
     2101 aaacggcgct tccgggctga tggtgtcgga gttgacgggg cagcagctct ctatggggac
     2161 gctccctctc tactacggcc cagcctccgg ccttccggga gccgcagtga agcttgcatc
     2221 cgggaatccg ttacgggccg ccttttttat tcctcgaaca catcccgggg cagcccttct
     2281 tcccccgctt ttgagcgcca gattcagagg ggcgcacgtt caggagtacg agacaggaac
     2341 ggcggtgctg ctcaccagga gtctcgaaga actcttcttt accggcgttc tgcccgatat
     2401 cgaagggctg ctgattgcgg caaagcacga tattcccgtt ttctacaggg ttgcgcctgc
     2461 gcttccgtcg gatacggatg cttctctggc gacgctcgga gcgcttttag cggccttccc
     2521 ccagattcgc accgtctccc cttccggcga agtcgctctt ggatacccga atcttcgccc
     2581 ccttgccgaa cgtatcgtca aggaggggcg ttccgtggcg atggtcgaat tttcccggca
     2641 gataggcgct ccccagctga actggctggc gtatccccgg cttgtttcgc tgcatagcgt
     2701 gaccaacgag gaaattttga gccgcaacat ctcccgtagt gcattgtacg aacgtttcgt
     2761 ccgggcggtg aaagagcgcg cggttcgcct tctcgtcttc cggccgtcgg ccatggaggc
     2821 gtccgacgct ccgtttgaac ggtttatcga agagttgcgt accctggcgt cgggtattac
     2881 cgcgtcgggc gcgcaactcc gctggccgga gccgttccag ccatggcgga cgggattcgt
     2941 cggcgccctt gcccttgcgc tcgcctttgt cttttctatg ctccgctttc ttcagaggtt
     3001 tttcaacggc ggaaccgatg accggaaaaa cgctgaagac gcgcttgcat ggcggacgac
     3061 gccgagaggg ctcgctgtct tttccgctat tgttctcctc gcgggtgtga tcattctcct
     3121 tcttccttcc gcaggccgct ttgccggagc cctgactgct gtcttcgtcg tcgtcgaggc
     3181 gtctttcctc gctcttgacg gctggaggac tccgtggctc ggcattatcg ggagtttcct
     3241 ttttgcagtc gccggagggt tggccatagc ggctttcttc agcgatcctg cgtcgatgct
     3301 tcggttgagg agcttttccg gggtgaaagc gacgctcttc ttgcctcccc tgctcgtgct
     3361 tttggctgat ctgaagcgga gagtgcatcc cgaatctctg ggagagctct ttcgaaggcc
     3421 gccgctgtgg ggtgagatat tccttctggt tcttttgctg ggaggcgccg gactcgttct
     3481 cttccgcagc gataatgtcc agttcgtccc cgcgttcgaa gtccgtatgc gggaattcct
     3541 ggaacgggcg cttgtggccc gaccgcggaa caaagaggtt ttcctgggct acccggcgct
     3601 cctcctctgg tatttcgtcc gcaaggcgga tatgtggcct cgctaccgcg aggtcttccg
     3661 cctggcgaca gtcattggtt tttcttcggc tgtgaacagc ttctgccact tccacactcc
     3721 gctcttcttt attctgttca ggcaattcaa cggattgtgg acaggggtgc tgctcggtat
     3781 gataagtatc gtctttcttc gcttcgttat catgcctctc tggtacaggt acggagggtt
     3841 ggtgacggag tgaggccgga acgggagttc cgcgttgcgc tctgcgggta ttacggattc
     3901 gggaatctcg gagacgaact gctcgccgag gcattgatcg cagcccttga acggtgcgga
     3961 atcaagcgcg gcgaaatggc gatgctctcc gcctccccgg aggaatctgc gcgcgctcac
     4021 ggggtctgct ctgtctcccg ctggtcgccg cgcgccgtat tccggattct tcggcggagc
     4081 gagacgtttc tcctcggcgg cggcggtctg tttcaggacg tcaccagcct tcgttcgccg
     4141 ctctattact ggggagcgct ccggcttgct ctcctcgcag gggcgcgccc ctggtgcgca
     4201 gggcagtccg tagggccgtt gctgacgaag agaggaagac tgctggcgcg gtcggcattg
     4261 gggagctgct cggtccgggg tgtgcgggat gcccggtcgg cggagcttct ggccgattgg
     4321 ggaatgaact ctgttgtaac gtgcgatccg gtcttttcgc tgactcctcc ggttacctct
     4381 ccccggcacg gaagcgcgtt gcttgtgaac atccgtccgt ggacgggagg actcgccagc
     4441 aaaactgcgg aagaatctgc tcgcctggcg tcttcccgtt ctctctccct cacggggttt
     4501 gcccttgcgg cggaggatgc cggcatcatg gaggagcttc gccgcagcgg agtttttccg
     4561 gcggagcgta ttgtcctcct cgacgcggag aactggagga gcgaatgcgg ccgtcttttt
     4621 ccggaagctg atggagtggt tgcgatgcgc ttccacgcgt cgcttcttgc gctgctcttc
     4681 ggcaggcctc tgacggcggt cgcctacgac ccgaaggtgg agtctcttgc ctccgaatgg
     4741 tcgcttcctt cgtggagcgg ctccggggct cttccggagc ctgcggcgcc gttaggtccg
     4801 ttccggcctt ttgaaaaggg cgcggagttc ctgaaaactt tctccgaaat gtgcgccgca
     4861 gcaatgcgac gcgatggaaa ggagtgaagg tatgcttcgt gaaccgtcct tcctcgcgga
     4921 aaaagcgtcg gagatccggc gcgatgtagt acgtatgatc ggtctggccc gttcggggcg
     4981 tctggcttct tctctttcga tagtggatat acttgcctgg ctctactggg acgtcctctc
     5041 cgtcgatcca gccgatccgt ggtttgagtc ccgggacaga ttcgtgctca gcaaggggcg
     5101 cggagcgcct gcgctttacg cgacgcttgc caacaggaag tttttcgagc gcgatgagct
     5161 gtggagctac cgccgtctgg gagcgatgct tcaggggtac ccgcaggcgc ttcgcattcc
     5221 cggcgtcgac gcccccggcg gcgggtcttt cggcatggga ttgggaattg ccaacggact
     5281 ggcgatcgcg ctccggcgcg ggcggtcgcc ctgcagggtc ttctgtctgg caggagaaga
     5341 ggagctgcaa gagggagcct tctgggaagc tgtgatagcg gcctcccgct tctgtctcga
     5401 cagtatctgc ctcgttatcg acaggtacag gacgaaggaa gaagctggaa tcccgtctgc
     5461 gtcggacgag gcgttctgcg aaaagatccg ctccttcggc tggacggcgg aagcgtgcga
     5521 cggacacgat ttttcctctc tggggggggc gttgcggctc ctcgggcaag gggcgggccg
     5581 tccgaagtgt ctgctcgcac gcactcgcct gggcaaaggg gtctcctttc tcgaaggcga
     5641 cagctccggc gaaagcgaag ttctggatcg ggagcgtgtg gataaggccc tcagagagct
     5701 tgagaaggag gaagatccgc tatgaacgcc gctggaagaa gcaccggaga cgcctacgga
     5761 cacgcgctca tctccatagc cgcctccgac gagaggatcg ttgcgcttga aggcggagac
     5821 gcggcgtgga gctcgccgtt ttcagaatcg tacccggagc gctgtttcaa tgccggaggc
     5881 gcggaacaga atcttattct gacggcagcc gggctcgctc tcggagggga taacgttttc
     5941 gtttcatcct atgcggcctt tctcaccggc aaagggtacg atcagattcg gacggcggtg
     6001 gccattccct cgctcccggt gcgcatcgcg gggctcggag cagggcttac ggccggcgaa
     6061 gagggcgcct gtcaccagac gctcgaagat atcgcgctga tgcgggtttt ccccggaatg
     6121 accgtcctcg ttccagcgga ttacaccgcc gcgttcgcgc tcttaaaaaa cgttgcaggc
     6181 gtgcagaagc cggtgtatat ccgccttggc gccgcggcga cgcccgatgt ctacgaggag
     6241 ggggacgaag cgttcttccc cggtggcgga cgcgttcttc gggagggaac cggcgtcacg
     6301 ctctgctgtt gtggtattat ggtccacgaa gcgcttcggg ctgcggatgt gcttgcccgg
     6361 cagaatataa gcgccgaggt tatcgactgt tacagtgttt cgcctcttcc cgtccaggct
     6421 attctcgatt ctgtccacag gacggggtgc tgcgtgactg cggaggagca cttcgcgagc
     6481 ggcggtctcg gagaggctgt cgcatcgctc acgggagcaa actaccccgt gccggtaaaa
     6541 atggtcgcgg tgttcgacaa gttcgggcag agcggttctc ctccggaact gaaggaatat
     6601 tacggcctga ccgcgtcgca gatagtg
//
LOCUS       DAOPKU010000116         6618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12360_length_6618_cov_5.930062, whole genome shotgun sequence.
ACCESSION   DAOPKU010000116 DAOPKU010000000
VERSION     DAOPKU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12360_length_6618_cov_5.930062"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /gene="ppk1"
                     /locus_tag="PK849_05025"
     CDS             complement(<1..1454)
                     /gene="ppk1"
                     /locus_tag="PK849_05025"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPE65520.1"
                     /translation="MLPTPSSKEGEVAEDMSPKKSADETLFEDIPKLDDPRLYLNREI
                     NWIDFNSKVLEEAMDPSTPMLEQLKFLSIFFNNLDEFFMVRVSALLEQYRSGVSSLAP
                     DGMSPSKQLALIRKRVLPLIAAAQEYWSKSLRPCLRDAGLRFVEYEDLGDKHKKFLEG
                     YFKNEIYPILTPQAIDPGRPFPVISNLSLNFLVQLADPQKGTRFARIKVPKNLSRFIF
                     LPRNKEAKSYESLGFSPNARQCDVLLVENLVAQHLGLLFPGLQVTASTTFRITRNTDV
                     EIEEDESSDLLEAVKDLVDRRNFGEVVRLEVSSGVPKDIFLFLVRNFRLAPFQIYKRK
                     GPLDFAGMMDLCSVDRPDLKDEPLLPRTPPPFSERAPLYAVLRKRDAVLYHPYDSFAP
                     VLDFVRRAAVDPKVVAIKQTLYRVGNESPVVDALMTARKNGKQVTAVVELKARFDEER
                     NINWAEALEDVGVHVVYGLVGYKIHAKMCLVIRR"
     gene            complement(1641..2444)
                     /locus_tag="PK849_05030"
     CDS             complement(1641..2444)
                     /locus_tag="PK849_05030"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPE65521.1"
                     /translation="MSSLKYRSVFLSDFHLGTRWCRAKSLVSFLGSMECEKLYLIGDI
                     IDGWKLKRSPGWPNSHNSVIRKILKMSKKTPVLYIPGNHDDFMKEFNGYSFGGVKICE
                     NAIHTSPEGKRFLVVHGHEFDVAVMYKPWLAFLGDRAYDISLHLNTGLNAIRSFLGFG
                     YWSLSEFLKRKVKDAVKFIGNYEECVAKAAGKYGVDGVICGHIHNPAIRRFKGIDYFN
                     CGDWVESCSALVEHLDGAMEIIRWFDKKSKKDEENVVFLEERSAVPAAD"
     gene            complement(2581..6168)
                     /locus_tag="PK849_05035"
     CDS             complement(2581..6168)
                     /locus_tag="PK849_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65522.1"
                     /translation="MRGKFKKIAGVLFVLCIAAFLAAFFLISGQDAGTSYFKTELEKA
                     VATLAGWTLETDGISGNFLAGYNAANIRILFEGQEVARAEELSVGLSILSFFRGQSGL
                     TKVTVTEGVLSGEGMLHALRRSDFSAGSGITADFMVPVILFSPVTITTPLGEISLASL
                     RLTPGEKTMTFHGEGHFLDFPVEAGASFTTGEQLSITDGFLRGGNAVVSFSGPLFPET
                     RIEGNIDDLRLDVISELTNLPFTAKGTVDSTLLLERPEGRLLVSGEGEIENGDIWDLL
                     MDGTFSWSADESKAVVAPVDARVFSSPASGVFALYFGEEPTAEIAFALKQLKIEEWTH
                     CFSWLSFAQGTLSTLKIDLSGPFEKLNGPILFEAPKATLEGFDVRALRGDLRMFDGER
                     IAISGNATWSDSPFTISGKSLLDDSAGAETSLSLHSGSFDLKSSGKVYAPALSADGKG
                     TMALEISIPSEGAASYKGALAAPRASFLGTAGEKLSVAFSGTEEILSLSALSFSPAGK
                     GLLSGKGSITGLSGKSPSITMEGSGRDLAWNLLRDALGGLETAGLFDASWSVKGPTAS
                     PVVSFGLKGRETPFSRSLPLRNIQLEGRFEKNELSVSKGSGSLFGGTANIEGKARFGS
                     APSLSFKGAFRGLSAGQIAAAAGADAETADGVVAGEFSLSGKPSSPLLSLTASAPSLG
                     VASLPLTAVRAKAEGTLSSLKITDFSARLQESSVDLSGVIGLSKGEKTNLKIAANKLD
                     LHSLAAAFFPGVRLGGTLSAQLNVSGKTGGAYTSTLSGTSPFLSVYGVLLEKAKVSLT
                     PDGEDAFDVALEGMLGESALALEGRAVVSPSGATFSLKNTKKIDLAKTASALSAHAAG
                     IFTGDADFSLTGKLGAVPTFEGSISAQSLGLYKTEARSVSVPFRWSDGMFVISGGKGV
                     YHEGTALFDGKVDPVTMRWEGNLAVKGMDLASASERILEGQGKISGKADLTMRGSGTG
                     GMLGLVFGSGQISAKEGSVSGFESIKSVSKTGEIRFSSLLASFNMDGRSIFLLPGSRL
                     SAPPGDTVYRYFSASGSVGWNDSPLDLKCVGDINIRALNAFIGALQGFLTIDGNPLTD
                     PQFLQRFLTGLVGGMAIRDFRETSFNLKGTWDSPEMLDLKVSRGSAPLTIPRSNGSTG
                     KNETQIKITVEIPTGSGSDTSASTEEQVKKQLLENIMKSIIKPGSTEND"
BASE COUNT         1395 a         1900 c         2003 g         1320 t
ORIGIN      
        1 tcgcgccgga tcacgaggca catcttcgcg tggatcttgt agccgacgag gccgtagacc
       61 acgtggaccc ccacgtcttc cagcgcctcg gcccagttga tgttccgctc ctcgtcgaag
      121 cgggccttca gctcgacgac cgcggtgacc tgcttgccgt tcttgcgggc cgtcatgagg
      181 gcgtcgacca cgggggattc gttcccgacg cggtagagcg tctgcttgat cgccaccacc
      241 ttcgggtcga cggccgcccg gcggacgaag tccagaacgg gcgcgaagct gtcgtacggg
      301 tggtagagca cggcgtcgcg cttgcggagg acggcgtaga gcggcgctct ctccgaaaac
      361 ggcggaggcg tgcgcgggag cagcggctcg tccttcaggt cgggccggtc cacggagcag
      421 agatccatca tgccggcgaa gtcgagcggc ccttttcgct tgtagatctg gaagggcgcg
      481 aggcggaagt tccgcacgag aaagagaaag atatccttcg gcaccccgga cgagacttcg
      541 aggcgcacca cttcgccgaa gttccgccgg tcgacgagat ccttcaccgc ttcgagaagg
      601 tcggaggatt cgtcctcctc gatctcgacg tcggtgttcc gcgtgatccg gaacgtggtg
      661 gaggctgtaa cctgaaggcc ggggaaaaga aggccgaggt gctgcgccac caggttctcc
      721 acaagcagga cgtcgcactg gcgggcgttc ggcgaaaagc cgaggctctc gtacgacttc
      781 gcctccttgt tgcgcgggag gaagatgaag cgcgagaggt tcttcggtac cttgatgcgc
      841 gcgaagcgcg ttcccttctg cgggtcggcg agctggacga gaaagttcag gctgaggttg
      901 gagatcaccg ggaaggggcg tcccggatcg atggcctgcg gtgtaaggat ggggtagatc
      961 tcgtttttaa agtacccttc gaggaatttt ttatgcttgt cgccgaggtc ttcgtactcg
     1021 acgaaacgca gtcccgcgtc ccgcaggcag gggcggagcg acttcgacca gtactcctgc
     1081 gcggctgcga tcagcggaag cacgcgtttc cggataaggg cgagctgctt cgagggcgac
     1141 atgccgtccg gcgcgagaga ggagacgccg ctgcggtact gctccagcag ggcggagacg
     1201 cgcaccatga aaaactcgtc gaggttgttg aagaagatgg agagaaactt gagctgctcc
     1261 agcatcggcg tggacgggtc catcgcctct tcgagcacct tcgagttgaa atcgatccag
     1321 ttgatctccc ggttcagata aagacgggga tcgtcgagct tcggaatatc ctcgaacaac
     1381 gtttcgtccg cactcttctt cgggctcata tcctccgcga cctctccttc tttcgaagat
     1441 ggcgtgggga gcatcgtaac gacctcctca ataacattat acacgtgcat ttcaacgctc
     1501 atggtacatg agcgcggttg gttacgccac agcggaatga gagaaccgaa aaactggtac
     1561 acttccgtaa tattggagag aaaaaacgtc cgcgcgggat cggcggacgg cgtgtgaacg
     1621 caggtatagg gcgagaggag tcaatctgct gcgggaactg cgctgcgctc ttccagaaag
     1681 acgacgtttt cctcgtcctt tttcgatttt ttgtcgaacc agcggatgat ctccatcgcg
     1741 ccgtcgaggt gctccacgag ggcggagcag ctttcgaccc agtcgccgca gttgaagtag
     1801 tcgatcccct tgaaacggcg gatcgccgga ttgtgaatat gtccgcagat gactccgtcg
     1861 acgccgtact tccccgcggc tttcgcgacg cactcttcgt agttgccgat gaacttcacg
     1921 gcgtccttga cctttcgctt cagaaactcg gagagcgacc agtacccgaa gccgaggaag
     1981 gaacgaatgg cgttcaaccc cgtgttcaga tgcagagaga tatcgtaggc acggtctccg
     2041 aggaaggcga gccagggctt gtacatcacc gcgacgtcga actcatgtcc gtggaccacc
     2101 aggaatcgtt tcccttcggg ggaagtgtgg atcgcgttct cgcaaatctt cacgccgccg
     2161 aacgagtagc cgttgaactc cttcataaag tcgtcgtggt tgccggggat gtagagcacc
     2221 ggcgttttct tcgacatctt cagtatcttc cggattaccg agttgtgcga gttcggccat
     2281 ccggggcttc tcttcagctt ccatccgtcg atgatgtcgc cgatcaggta gagcttctcg
     2341 cactccatcg acccgaggaa tgacaccaga ctcttcgctc tgcaccagcg cgttccgagg
     2401 tggaaatcgg agaggaagac ggaccggtat ttcagagaac tcatcccttt cttctctttt
     2461 cttgcgtcat agcataactc ctttcggtaa cggaagttcc gtgcgaatga tacgaaaatg
     2521 tatcggttgt atctccgtac gtcgactcca accgtataag tatacctctt ttgcagccgc
     2581 tcagtcgttt tccgtcgagc cgggtttgat gatggacttc atgatgttct ccagaagctg
     2641 tttttttact tgctcctccg tgctcgccga ggtgtccgac cccgaccccg tggggatctc
     2701 cacagtaatc tttatctgcg tttcgttctt gccggtggag ccgttggacc tcggaatggt
     2761 cagcggagcg ctcccccggg ataccttcag gtcgagcatc tccggagagt cccacgtccc
     2821 cttgaggttg aacgaggtct cccggaagtc gcggatcgcc attccgccga cgagccccgt
     2881 caggaagcgc tgcaagaact gggggtccgt cagcggattg ccgtcgatgg tcagaaaccc
     2941 ctgaagagcg ccgataaatg cgttgagcgc cctgatattg atgtcgccga cgcatttcaa
     3001 gtcgaggggg gagtcgttcc accctacgga gccgctcgct gagaagtagc ggtagacggt
     3061 gtctcccgga ggggcggaga gtctgctgcc cgggagaaga aagatgctgc gtccgtccat
     3121 gttgaagctt gcgaggagcg acgagaatcg gatttcccct gttttcgaca ccgatttgat
     3181 cgactcgaac cccgagacgg aaccctcctt cgcagaaatc tgcccgctgc cgaagacgag
     3241 tccgagcatg ccgcccgtgc cgctcccccg catggtgagg tcggcctttc cggagatctt
     3301 cccctgccct tcgaggatgc gttccgaagc gctcgcaagg tccatcccct tcacggcgag
     3361 gtttccctcc caccgcatgg tgacagggtc gactttcccg tcgaagaggg cggttccctc
     3421 gtggtagacc cccttgcccc ccgaaatcac gaacatgccg tcgctccagc ggaacgggac
     3481 ggagacactg cgcgcctccg ttttataaag tcccagcgat tgggcggaaa tggagccttc
     3541 aaacgtcggg acggcgccca gcttgcctgt gagcgaaaag tccgcgtctc cggtgaagat
     3601 gcctgcagcg tgcgccgaaa gcgcggatgc cgtcttcgca aggtcgatct ttttcgtgtt
     3661 cttgagcgag aatgttgcac ccgagggcga aacgaccgct cgtccttcaa gcgcgagggc
     3721 gctctccccg agcattcctt caagagcgac gtcgaacgcg tcctctccgt ccggcgtgag
     3781 cgagactttt gccttctcaa gcaacactcc gtagacggag agaaagggcg acgtgccgga
     3841 gagagtcgac gtgtacgcgc ctcccgtttt tccggagacg ttgagttgcg ccgagagcgt
     3901 tccgccgaga cgtaccccgg gaaagaacgc cgcggcgagc gaatggagat cgagcttgtt
     3961 cgccgctatc ttcagatttg tcttttctcc tttgctcagg ccgataacac ccgaaagatc
     4021 aacagaagac tcctgcagtc tggccgagaa atccgtgatc ttcaaagatg agagcgttcc
     4081 ctcggccttt gcccggacag ccgtcagggg caaggaggca acgccgagcg agggagcgga
     4141 cgccgtcagc gacagcaagg gggaggatgg cttccccgaa agggagaact ctccggcgac
     4201 caccccgtcc gcggtctccg catcagcgcc tgccgcggct gctatctgtc cggcggagag
     4261 ccctctaaag gctcctttga aggagagcga cggcgccgag ccgaaacgcg ctttcccctc
     4321 aatattcgcc gttccgccga agagagagcc cgagcctttg gaaacggaaa gctcattctt
     4381 ctcgaagcgc ccttcgagct ggatgtttct gagagggagc gagcgggaga acggcgtttc
     4441 tctgcctttg aggccgaacg agacgaccgg agaggctgtc ggccccttca cgctccatga
     4501 ggcgtcgaag aggcccgcgg tctcaagacc gcccagggcg tcccgcagaa ggttccacgc
     4561 gaggtcgcgt ccgcttccct ccatggtgat ggacggcgac tttccggaga gtccggtaat
     4621 gctccccttg ccggagagaa gccccttgcc ggcgggagag aaagagagcg cggagagaga
     4681 aagaatctct tcagtacctg aaaaagctac ggaaagcttc tctcctgccg tacccagaaa
     4741 cgaagcgcgg ggcgcggcaa gagcgccttt gtacgatgcg gctccctccg aaggtatgga
     4801 gatttcaaga gccatcgtac ctttcccatc ggcggagagc gcgggagcgt agacctttcc
     4861 ggaggacttc aggtcgaagc ttccggagtg aagagaaagg gaggtctctg cgcctgccga
     4921 gtcgtcaaga agactcttcc ccgagatggt gaaaggggaa tcagaccacg tcgcattccc
     4981 ggagatggca atgcgctccc cgtcgaacat gcgaagatct ccgcggagcg cgcggacgtc
     5041 aaagccttca agagtcgcct tcggcgcttc gaagagaata ggtccgttca gcttctcgaa
     5101 aggaccggaa aggtcgattt tgagcgtcga gagcgttcct tgggcgaagg agagccagga
     5161 gaagcagtgc gtccactctt caatcttcag ctgtttcagt gcaaacgcaa tttccgccgt
     5221 cggttcctcg ccgaagtaga gggcgaagac gccggatgcg ggcgaggaaa aaacacgtgc
     5281 gtcgacggga gcgacaacgg ctttcgactc gtccgcagac catgagaacg taccgtccat
     5341 cagcagatcc cagatatcgc cgttttcgat ctccccctct ccggagacga gcaaacgtcc
     5401 ctcggggcgt tcgagcagga gtgtggagtc gacggttccc tttgcagtaa agggaaggtt
     5461 cgtcagttcc gaaatgacgt ccagccgaag atcgtcgatg tttccctcga tgcgcgtctc
     5521 cggaaaaagc ggcccggaga aggagacaac ggcgtttcct cctctgagaa aaccgtcggt
     5581 gatggaaagc tgttcgccgg tggtaaacga ggcgcccgcc tcgaccggaa aatccaggaa
     5641 atgcccctct ccgtgaaagg tcattgtctt ctcccccggc gtcaggcgga gcgaagccag
     5701 cgagatctct cccaaggggg tcgtgatggt tacgggagag aacaggatga ccggaaccat
     5761 gaagtcggcg gttatgccgc ttccggcgga gaaatccgag cgacggagcg cgtggagcat
     5821 cccctctcct gagaggacgc cctccgtcac ggtcaccttt gtcagaccgc tttggccacg
     5881 aaaaaacgag agaatggaaa gccctacgga aagctcttcc gcgcgggcaa cctcctgccc
     5941 ttcgaagaga atgcggatat ttgccgcgtt gtaccctgcg aggaaatttc ctgaaatacc
     6001 atccgtctcc aacgtccatc cagcaagcgt ggcgacggct ttctcaagtt ccgtcttgaa
     6061 ataggacgtc ccggcatcct gcccggagat caggaagaat gccgcaagga aagcggcaat
     6121 acagagaaca aacaataccc cagcaatttt tttgaatttc ccgcgcatac gctctaccct
     6181 ttcccggagc acaccctcgg cgtgcaatgt aaaaaccata ctataatatg caatcacacg
     6241 gggtaattgc aaggcaatac tactgaaaag aggatgaaga gaaaaaagcg cgcgctgtac
     6301 aacctatata ttgagcctct aagggggcgg gggtattgtg gatatgtgga aaaaactgtg
     6361 gataacgggt gaaaaacagg gcgaaagaac aggcctcgcg cgattttgtg gataacatgg
     6421 acttctgtgg atagaaatca cggcacacta cggctcgggc gcagcgcgtt actcggtact
     6481 ccgtgatacc tgaatttctg aagcgacagg gagttcgcac ccgttgtacg agagaaggag
     6541 ctttccgacc gcgcggatac ggtctccctc gcgaagcgag agcagactct cggtctcggg
     6601 acttttgtag gcgacgac
//
LOCUS       DAOPKU010000117         6606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12383_length_6606_cov_5.144558, whole genome shotgun sequence.
ACCESSION   DAOPKU010000117 DAOPKU010000000
VERSION     DAOPKU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12383_length_6606_cov_5.144558"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PK849_05040"
     CDS             <1..863
                     /locus_tag="PK849_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPE65523.1"
                     /translation="PGHIRTCPGGVARNIAENLARLGVPVALITALGDDAGGASLRAS
                     CRELGIRLDRSVEVPGHPSSTYMAIMDEEGDMALGLSDMRILDHVTPEHLESCAPLLS
                     EADVVAADACLTTETLERLVNGPCAKVFIDPVSIGKCRRMERLLGRFYCVKMNRMEAA
                     YLAGMTIETGGDLDETARRMLASGVKKLYITLGAEGVFFAEEGNSGRVPAIPLLPVNA
                     TGAGDAFTAAAVYGELHGWTMERTARFAVKASALTLLSRDTVSERMSMAAVEAVGGAA
                     LPGDKGERFS"
     gene            860..1801
                     /locus_tag="PK849_05045"
     CDS             860..1801
                     /locus_tag="PK849_05045"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPE65524.1"
                     /translation="MNERSILNERVRIAPEAEEALAGGRAVVALESTIISHGMPYPEN
                     VESARTCERIIREEGAVPATVAVIGGIIRVGLSEEELEHMGRGGASIRKVSRRDLPML
                     LAKGLDGATTVATTMIGASLVGVRVFATGGIGGAHRGAPQTFDVSADLTELARTPVAV
                     VCAGAKSILDIGLTLEVLETYGVPVVGYRTDAFPAFYVRNSGFRVDERADDLREIARA
                     LRLKWSLGLEGGMVVANPIPEEDEMDPALIARSIDEAVAAATARGVRGKEITPFILAY
                     LHRTTGGSSLHANKQLVYNNARVAAKLAVAYAEEAGE"
     gene            complement(1777..2100)
                     /locus_tag="PK849_05050"
     CDS             complement(1777..2100)
                     /locus_tag="PK849_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65525.1"
                     /translation="MTNGARKNVRSTWSGSADSSARVLGQSAYLLFRKVDEFGDEGKR
                     HALIQHAPDRLELRQFDAFFASFFPTFLPTFFSPLFSSFFFEFPDFLLQIGQHFVPYS
                     PASSA"
     gene            2406..2651
                     /locus_tag="PK849_05055"
     CDS             2406..2651
                     /locus_tag="PK849_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65526.1"
                     /translation="MNDSKTLAILLEFVQSPLVEGNYHIYKFDPQRVEKSDPELAEQI
                     VRLGEATERALEHLAENGIHVIAGGALPPYLTSSMLN"
     gene            2738..3829
                     /locus_tag="PK849_05060"
     CDS             2738..3829
                     /locus_tag="PK849_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65527.1"
                     /translation="MKKLYGTLLLSLCLLLLSNAAGNAAETLDNRLAELRGIENRGDL
                     DHLGLEKLRTLGGTEPKDILSLPQADILWLLSYGLDMYDSMLYSLVGKSERGARRQDE
                     KLGEFTDRFNTIWNTINSAYTALSEKSTEPSPADKLFDLLKKGASPQELLPVTTEGLK
                     YTWEIVKGWFAESEKEEAFLTELKTLRAENAALLENAPDSPWNFALLSYARLRRQSAD
                     YVKYLNQFEIEIARHPVPYEKTRVLNDGILSIKQELAAYDAKLRKAVVDIPVFTVQFY
                     SKARIDSAKGIFSDDTAAVLEAGALFARTAPLINLAVDDNVRKGWTEIAGALKDLLNE
                     EQYDELKSISQAVNMMWDFDDVPRTINWP"
     gene            complement(3883..4206)
                     /locus_tag="PK849_05065"
     CDS             complement(3883..4206)
                     /locus_tag="PK849_05065"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPE65528.1"
                     /translation="MGEKYNIRYLELARSDLLGIAEYVNSQAWERDAANRLVDSLEKA
                     ILRLESFPYSGALYGKSFGFKEEHRMLVVGNYLVFYVVYDDFVEIRRVIHGKRRFFDL
                     LQPLS"
     gene            complement(4190..4468)
                     /locus_tag="PK849_05070"
     CDS             complement(4190..4468)
                     /locus_tag="PK849_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPE65529.1"
                     /translation="MPHIRPVSDLRNKFAEISKIVHECQEPIFLTKNGYGDMVVMSIE
                     QFERLQFENEIVFKLREAENQSSADDRRYSHADVFDALRERLADGRKI"
     gene            4696..5871
                     /locus_tag="PK849_05075"
     CDS             4696..5871
                     /locus_tag="PK849_05075"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65530.1"
                     /translation="MYIPRKMEKTALQLMEGFPCLAVTGARQSGKTTLARYLFPEKPY
                     VSLEDPDQREFAENDPRSFLDRFPDGALFDEAQRAPSLFSYLQGRVDLDGRMGLFVLN
                     GSQHFHLLENITQSLAGRTALLQLPPFSFDELRSAGLLPESADEAILSGGYPPLHTRK
                     VERTAWFANYTATYLERDVRSMLNVRDLSTFQRFLRLCSGRAGQLVNFSSLAGECGVV
                     HNTAKAWLSILEAGSIVFLVRPYFRNFNKRLLQTPKLYFHDTGLLCHLLGIRNEEQLE
                     THPLRGAVFENMVAVELLKARLNEGREPELYFWRDRSGFEIDFLIENDGLLDAIEVKS
                     GKTVTREFFRAIGRWKEIAGESAGKTFLVYGGDEEYEREGHSVLSWRNAGGIAVACR"
     gene            6042..6314
                     /locus_tag="PK849_05080"
     CDS             6042..6314
                     /locus_tag="PK849_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65531.1"
                     /translation="MKMVSVQEIKKNPAVLYSGPGYRVLTSNNRPKALCFPLDELDDI
                     EEIVASFRQAQAMRAVERVRSFAVEKGLDGMTMEDVDEEIRRARNG"
BASE COUNT         1454 a         2015 c         1924 g         1213 t
ORIGIN      
        1 atcccggaca catccggacc tgccccggcg gggtggcgcg gaacatcgcg gagaacctgg
       61 cgcgcctcgg cgttcccgtc gccctgatca cggcgctcgg ggacgacgcg ggcggagcga
      121 gccttcgcgc ctcctgccgc gagctcggca tccgcctcga ccgtagcgtg gaggttccgg
      181 gacacccctc ctcgacctat atggcgatca tggacgagga gggggacatg gcgcttggcc
      241 tctccgacat gcgcatcctc gaccacgtga cgcccgaaca tctggagtcg tgcgcccccc
      301 ttctgtccga agccgacgtc gtcgccgcgg acgcctgcct gaccacggag actcttgaac
      361 ggctggtgaa cggaccgtgc gcgaaggtct tcatcgaccc cgtctccatc ggcaagtgcc
      421 ggaggatgga gcgccttctc ggtcgtttct actgcgtcaa gatgaacagg atggaggccg
      481 catacttagc cggaatgacc atcgaaaccg gcggcgacct cgacgagacc gcccgccgca
      541 tgctggcgtc gggggtgaaa aaactgtata ttacccttgg agccgaaggc gttttcttcg
      601 cggaagaggg aaacagcgga agggttccgg ccatcccgct gcttcccgtg aacgccaccg
      661 gcgccgggga cgcgtttacc gcagccgcgg tctacggcga gctgcacgga tggacgatgg
      721 aacggacagc ccgcttcgcg gtgaaggcct ccgcgctcac gctcctgagc cgcgacacgg
      781 tgagcgaacg gatgagcatg gccgccgtcg aggccgtcgg aggcgccgcc ttacctggag
      841 ataaaggaga gcgattctca tgaacgagcg cagtatttta aacgaaagag tccgcatcgc
      901 accggaggcg gaggaggcat tggccggggg gagggccgtc gtcgcgctgg agtccaccat
      961 catcagtcac ggcatgccct accccgagaa cgtggagagc gcccgcacct gcgagcggat
     1021 catccgcgaa gagggagccg tccccgcgac cgtcgcggtc atcggaggga tcatccgcgt
     1081 cggcttgagc gaagaagagc tggagcacat ggggcgcggc ggcgccagca tccgcaaggt
     1141 cagcagacgc gaccttccca tgctcctggc caaggggctc gacggcgcga ccactgtcgc
     1201 cactacaatg atcggcgcat cgctcgtggg cgtgcgcgtc ttcgccaccg gcggcatcgg
     1261 cggcgcgcac cggggcgctc cccagacgtt cgacgtctcg gccgacctga cggagctggc
     1321 ccgcacaccg gtcgcggtgg tgtgcgccgg ggccaaatcc atcctcgaca tcggcctgac
     1381 cctcgaagtg ctggagacgt acggcgtccc cgtcgtgggg taccggacgg acgccttccc
     1441 cgccttctac gtgcgcaaca gcggcttccg cgtcgacgag cgggccgacg acctgcgcga
     1501 gatcgcccgc gcgctccggc tgaagtggtc gctcgggctg gagggaggca tggtcgtcgc
     1561 caaccccatc cccgaagagg acgagatgga ccccgccctg atcgcgcgca gcatcgacga
     1621 ggccgtggcg gccgcaacgg cgcggggcgt ccgcggaaag gagatcacgc cgttcatcct
     1681 cgcctacctt cacaggacga cagggggaag cagcctccac gcgaataagc agctcgtcta
     1741 caacaacgcc cgcgtggcgg cgaaactcgc tgtggcctac gcggaagagg cgggggagta
     1801 ggggacgaag tgctgaccta tctggagaag gaaatccgga aactcaaaaa agaaggacga
     1861 gaagaggggc gagaagaagg tcgggaggaa ggtcgggaag aaggacgcaa agaaggcgtc
     1921 gaactggcga agctccagac ggtccggcgc atgctggata agggcatggc gctttccctc
     1981 gtcgccgaac tcgtcgacct tccggaagag caggtacgcc gactggccga gcaccctcgc
     2041 tgaactgtcg gccgaaccgc tccaggttga cctgacgttt tttcttgcgc cgttcgtcaa
     2101 gaggtttccc gcattcgggc tcttgacgaa cggcgcggct attgctacta ttaatttcca
     2161 tttgttgtat atctctcatt accctctcag gcagacggct ttcggaccat ccttcccggc
     2221 ggaggatgaa gcggagcaag aaaatttcca gatctcactg aacgatgcac acgacccaaa
     2281 aacactggac atgatgtgtg ttcggttccc gcaacgtccg tcgttactct tcacgcgcac
     2341 agcttcggtt cgggcggaac gacgccctga agcaccttga taagagaacg gaggaggtat
     2401 cccgaatgaa cgattccaag acgctcgcga ttttgctgga gttcgtccaa agcccgcttg
     2461 tggaaggcaa ctaccacatc tacaagttcg acccgcagag agtggaaaag agcgaccccg
     2521 aactggccga gcagatcgtc cgtctcgggg aagccaccga aagagccctg gaacacctcg
     2581 cggaaaacgg catccacgtg atcgccgggg gcgcgctgcc cccttacctc acgtcgagca
     2641 tgctcaactg aagccacgtg aaaccggagc gggaaagaga tgcttctccc gctccggagg
     2701 ttcaatgagt aaaaatcacc ttcaggagga atgcgttatg aaaaaactgt acggaacgct
     2761 gcttctctcc ctctgcctgc tgctcctgtc gaacgcagca ggaaacgccg ccgaaactct
     2821 ggataacagg ctcgccgaac ttcgcggcat agaaaaccgg ggcgatcttg accatcttgg
     2881 actggaaaag ctaagaacgc tcggcggaac ggagccgaaa gacatcctct ccctgccgca
     2941 ggcggatatc ctctggctcc tctcctacgg cctcgatatg tacgactcca tgctctactc
     3001 cctcgtcggg aaaagcgaac gcggagcgcg cagacaggac gaaaagctcg gcgaattcac
     3061 cgaccggttc aacactatct ggaacacgat caactcggcg tacaccgcgc tttcggaaaa
     3121 atcaacggaa ccttcgcccg ccgacaagct cttcgacctg ctgaaaaagg gcgcttcccc
     3181 gcaggagctg cttccagtaa ccaccgaagg cctgaaatac acgtgggaga tagtcaaggg
     3241 gtggttcgcg gaaagtgaaa aagaagaggc gttccttacc gaactgaaga cccttagagc
     3301 cgagaacgcg gcgcttctgg agaacgcgcc ggacagcccg tggaacttcg cgctgctgag
     3361 ctacgccaga ttgcggcggc aatccgccga ctacgtcaag tatctgaacc agttcgagat
     3421 cgaaatcgcc cgacaccccg tcccctacga gaagacgcgc gttctcaacg acggcatcct
     3481 ctcgatcaag caggagctgg ccgcatacga cgccaagctg cggaaggccg tcgtcgacat
     3541 ccccgtattc accgtccagt tctactccaa ggcgcgtatc gacagcgcaa agggaatttt
     3601 cagcgacgac acggcggccg tactggaggc cggagcgctc ttcgcccgga cggcgcccct
     3661 gatcaacctc gccgtggacg acaacgtccg gaaagggtgg acggagattg cgggcgcgct
     3721 gaaagacctg ctgaacgagg aacagtacga tgaactgaag tccataagcc aggcggtgaa
     3781 catgatgtgg gacttcgacg acgtccccag gacgatcaac tggccgtaga cggagtacgg
     3841 agcgcggaag gggctttccg aagacccctc cgcgccgcgc tgtcacgaaa gcggctgaag
     3901 aaggtcgaag aaacgccgct tcccatgaat aaccctgcgt atttccacaa aatcatcgta
     3961 cacgacataa aagacgaggt aattcccgac gacaagcatt ctatgctctt ctttgaaacc
     4021 aaaagatttt ccgtagagcg ctccgctgta cggaaagctt tcaagacgca agatcgcctt
     4081 ctccaaagag tctaccagcc ggttcgccgc atctctctcc caagcctgcg aatttacata
     4141 ttcggcaata cccaaaagat cggaacgcgc cagttcgaga tacctgatat tatatttttc
     4201 gcccatcggc cagacgttcc cgaagcgcat caaacacatc ggcgtggctg tatcgcctgt
     4261 cgtcggcgct tgactggttt tccgcctctc tcagcttgaa gacgatttcg ttttcaaact
     4321 ggagcctttc aaactgctcg atactcatca caaccatatc gccgtacccg ttcttcgtaa
     4381 gaaaaatcgg ctcctgacac tcgtggacaa tcttcgaaat ctccgcgaat ttgtttcgca
     4441 gatccgaaac agggcgaatg tgcggcataa aatcacctcc tgatgaggat agcgcaattt
     4501 tatcataatg aggatatttc cgtgcggatt cgatgcttcg gagaataaac ggaacgacct
     4561 cgtcacggtc gactttcagg tcaaccacgg aataccgcac tacagctgat tggtaattga
     4621 attgacaatc agccgggaat cgagtaccct cctctttgta cagtgccttt ccgtcgatga
     4681 acgaggtgat gccggatgta tatcccgcga aaaatggaaa aaacggcctt acaactgatg
     4741 gaaggcttcc cctgtcttgc cgtgaccggc gcgagacagt ccggaaagac cacgctcgcc
     4801 cggtatctct ttccggagaa accgtacgtc tcgctggagg atcccgacca gcgcgaattc
     4861 gcggagaacg atccccgctc gtttctggat cgcttccccg acggcgctct cttcgacgaa
     4921 gcgcagcgcg ccccgtcgct cttttcctat cttcaggggc gggtcgacct cgacgggagg
     4981 atggggctct tcgttctcaa cggctcgcag cactttcacc ttttagagaa catcacgcag
     5041 agccttgcgg gcagaacggc gctcctgcag ctccctccct tctctttcga cgaactgcga
     5101 agcgccgggt tgctccccga aagcgccgac gaagcgatct tgtccggcgg ctatcctccg
     5161 ctgcacaccc gcaaagtgga acgcaccgca tggttcgcca actataccgc gacctacctt
     5221 gagcgcgatg tacgctcgat gctcaacgtc cgcgacctct ccacttttca gcgcttcctt
     5281 cgcctgtgct ccgggagggc cgggcagctc gtcaacttct cttctctggc gggggagtgc
     5341 ggcgtcgtcc acaacaccgc caaggcgtgg ctttccatcc tggaggcagg ctccatcgtc
     5401 tttcttgtcc gcccgtactt ccgcaacttc aataagcgcc tcctccagac cccgaaactt
     5461 tacttccacg acaccgggct gctctgccac cttctcggta tccggaacga agagcagctc
     5521 gaaacacacc ccctgcgagg cgccgtcttc gagaacatgg tcgccgtcga gcttttgaag
     5581 gcgcgcctca acgaagggcg cgaacccgaa ctctatttct ggcgcgaccg aagcggcttc
     5641 gagatcgact tcctgatcga aaacgacggt cttctggacg cgatcgaggt aaaatccgga
     5701 aaaacggtga cccgcgagtt cttccgggcg atcggacgct ggaaggagat cgccggcgag
     5761 agcgccggaa agacgtttct ggtctacggc ggagacgagg agtacgaaag ggaagggcat
     5821 tcggtcctct cgtggagaaa cgccggcggc atcgcggtgg cgtgccgatg acagtcgcgc
     5881 atgctcagcg acgatccgca ggagcgcgag ctgcttgatt tcatcgaaac cgcaacggat
     5941 acggagagtt gggagtgaaa acgagatacg ccccctgttt cgagatacaa taggggtgct
     6001 gatgtagcat atatcgaagg attaaacgga ggtggttgat cgtgaagatg gtgtccgttc
     6061 aggagatcaa aaagaatccg gcggttcttt acagcggccc cggataccgt gtgctgacat
     6121 ccaataatcg cccgaaggcg ctctgtttcc ccttggatga gttggacgac atcgaggaga
     6181 tcgtggcgag ctttcggcaa gcccaagcga tgagggcggt ggaacgagtg cgctccttcg
     6241 ctgtcgagaa gggactcgac ggaatgacta tggaagatgt cgacgaggag atacgtcgcg
     6301 cccgtaacgg ttagctcagt ctcatgctct aagaagatat gctccatcct tttcgaactc
     6361 attgtgcccc tgatcaactc caccgcgaac gatcgacttt tcctggaaag ctccatgaaa
     6421 agcgggaaga ccattcgcag acccctccgc tcttcagccc aattcatacc ggcctgttca
     6481 tgctatactt attccaatta ccaagccaag aaccaatttc actttccggg gaggtctttc
     6541 tatgcccgtt gctctcggaa acagtctcag ggaggccatt cgttcggctc gctccggggg
     6601 gagaac
//
LOCUS       DAOPKU010000118         6601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12399_length_6601_cov_4.639322, whole genome shotgun sequence.
ACCESSION   DAOPKU010000118 DAOPKU010000000
VERSION     DAOPKU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12399_length_6601_cov_4.639322"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PK849_05085"
     CDS             <1..391
                     /locus_tag="PK849_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPE65532.1"
                     /translation="GVAARLFSDLAAAGVGAEMIVQSVMRGQMNDIAFIIKQSLLGEA
                     IAICRSFASDIGAQGVTFDTEVAKVALKGEHLAGCPEIPSQMFSVLAGCRINIDMIAA
                     ASTVIACIVAAGDLEGAVGALSQAFLH"
     gene            469..1212
                     /gene="lptC"
                     /locus_tag="PK849_05090"
     CDS             469..1212
                     /gene="lptC"
                     /locus_tag="PK849_05090"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPE65533.1"
                     /translation="MKLFRCIGALLALLLCLGAASAREVKLTADTMQYDSASGYFTAT
                     ENVKIVREGVTATAKKAEGNMNGGEVNLIGDVHIFGVWEDEKVDIKGQILTGYLQDPR
                     GLSFKGTVQGVWGSKQVDAEDLSTRGDTFSVAKLRRFADSAEGFSFSCNSIKGKLKDG
                     EIVEFTAVGAFRFVSSQKKGTPTEIRGDRAVYSKARGSLVVNGNVSAVQDGRSLRSDS
                     LVYFPVKNRIEATGKPQLIFKIDEGSKKQ"
     gene            1209..1934
                     /gene="lptB"
                     /locus_tag="PK849_05095"
     CDS             1209..1934
                     /gene="lptB"
                     /locus_tag="PK849_05095"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPE65534.1"
                     /translation="MSKMLSARGLSKAYKGRTVVSSADLDVAMGEVVGLLGPNGAGKT
                     TSFYMIVGLIKPDAGVVTVDSRDLTDLPVYRRARLGVGYLPQEASVFRNLSVRENIEL
                     VLQESPPPGENPSIVADRLIEDFGLEKIAHVRGYSLSGGERRRVEIARSLAILPNFLL
                     LDEPFSGIDPIAVYDIQQIILGLRAKGYGILLTDHNVRDTLAITDRTYLIHQGKIVIE
                     GSPDEVAESEVARKFYLGEQFSW"
     gene            complement(1907..2386)
                     /locus_tag="PK849_05100"
     CDS             complement(1907..2386)
                     /locus_tag="PK849_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPE65535.1"
                     /translation="MRHTVLWCVGNPLLQDDGAGPALFTILEGVAPGQLRVVNCETTP
                     ENYIAPLTRAIRNDSVHLLIVDAAEMGLPGGAVRRMQRQRYSVRSEGDAGIPLPLLLA
                     PLLPELEVTVLGIQPARRGFGDSLSPEVSLAVETLAPLLLEGRWDEVEAYQENCSPR"
     gene            2447..4033
                     /gene="murJ"
                     /locus_tag="PK849_05105"
     CDS             2447..4033
                     /gene="murJ"
                     /locus_tag="PK849_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPE65536.1"
                     /translation="MAKSTHTMVRHALSMMLGTFASRVLGLVREIITAAWFGASGVLD
                     AFNVSFTLANLARQLLAEGALSASFVPVFSRVLAAKGKESAERLARQAFSVLLVATIL
                     SVAAGVVFSPLLVKIMAPGFDPVKAELATAMTRWMFPFLVLVSLAALAMGVLNSMGSF
                     LVPALAPALSNLVYIVLVVFLASFYGVWGLVIAVLAGGACQFLLQWAWSVRMGVTLLP
                     EWPQLKDPDLRTMLALFLPYAAGLSLNQVNPVISRMLASFLQEGAISVLNYANRVIQL
                     PLGIFVIAISQAVLPQLSRCPAEDTGEFRDIMRDSLRFTLFVVFPATLGLALVSDEIV
                     HLLFVRGAFGEWAWKGTSVSLAMYSLGLPGMAFSTVVMRGLYARSLPRGALIVTLSSV
                     AATLLFSLALMGPLSFGGLALATSLAFSLSGWVGVRILSRHLGMRFGIFSVPWTAKMG
                     VSLAFTGLIVVAWRWFFPYSPESSGILRAAWVGGVFCAAASGYAAVTALLRFQEWRWL
                     FGALRRKDRRETPGQNAKEE"
     gene            4037..4564
                     /locus_tag="PK849_05110"
     CDS             4037..4564
                     /locus_tag="PK849_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65537.1"
                     /translation="MTFRKLLLCVCVLALLLPVQSFAGDVESLLEKRSAILWFEGEEF
                     GELIIGARAQFAFVYVDGALSEAVVKEPGAPDWLRDNIYYYGSKETRKKALFIVRLRT
                     VKNLDLQLSMIKIGTHTLTPEDVLTNKHYVPVGELPPDFSAAFAVAVPLAAVKGTTRT
                     VSVGDYGTELEFPIR"
     gene            4561..5589
                     /gene="tsaD"
                     /locus_tag="PK849_05115"
     CDS             4561..5589
                     /gene="tsaD"
                     /locus_tag="PK849_05115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPE65538.1"
                     /translation="MSLPEESFLTLGMESSCDDTAVAVLRGGRRVEAELLSSQLEDHV
                     PFGGVIPEFAARRHLEAFLPLTEEALRRAGVADPRREIGLVAVTAGPGLMGSLLVGVM
                     AAKALSLAWDVPLLGVNHLEGHIFANVVSHPELSPPFLCMVVSGGHTEIVLVKKFGTY
                     IFLGGTRDDAAGEAYDKVAKVLGLPYPGGPVVDKLAASGNPDAFDFPSPLKGSPEIVF
                     SFSGLKTAVLTRIKKLRNEGIEIPVEDVCASFQRAVTDSLMTKLRLASRRTGVRAVSI
                     SGGVAANSALRNALLQEKGWKVFLPPKNRCTDNAVMIAAAGYSAFRRGARSSLDLAPD
                     PSWELWKK"
     gene            5787..6077
                     /gene="groES"
                     /locus_tag="PK849_05120"
     CDS             5787..6077
                     /gene="groES"
                     /locus_tag="PK849_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPE65539.1"
                     /translation="MNLKPLGDRLVVKVLHNEEKTKGGIVLPDTAKEKPTEGEVIAVG
                     TGKVLENGQKLPLEVKVGDRIIFSKYAGTEVKLDGDEYVIFSERDVLAIIDK"
     gene            6106..>6601
                     /locus_tag="PK849_05125"
     CDS             6106..>6601
                     /locus_tag="PK849_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPE65540.1"
                     /translation="MAKILIFGEEARRAMLRGIDKVADTVGVTLGPKGRNVVLEKKFG
                     SPTITNDGVTIAKEIELEDVYENTGAQLLKEVASKTNDIAGDGTTTATVFARAIIAEG
                     MKNVAAGANGMQMRSGIERAIDLVVDELKKKAISVKNRDKIAQVAAISANDKAVGQLI
                     AEAMD"
BASE COUNT         1233 a         1948 c         2052 g         1368 t
ORIGIN      
        1 cggcgtcgcg gcgcgtctct tctccgatct tgccgcggcg ggagtgggag cggagatgat
       61 cgtgcagagc gttatgaggg ggcagatgaa cgacatcgcc ttcatcatca aacagagtct
      121 cctgggcgag gccatcgcga tctgcaggtc tttcgcctcc gatatcgggg cgcagggcgt
      181 gaccttcgac acggaggtcg cgaaggtggc gctcaaggga gagcatcttg ccgggtgtcc
      241 ggagatcccc tcgcagatgt tctcggtgct cgcggggtgc aggatcaaca tcgacatgat
      301 cgcggcggcg tccaccgtca ttgcgtgcat cgttgcggcg ggcgatctgg aaggagcggt
      361 cggcgcgctc tctcaggcct ttctgcactg attacgctgt tccgtacgga cgaagtgctg
      421 taaaatatca aggctgtccg ggaaattttg tgtgtgggag ggagcctcat gaaactgttc
      481 cgatgtatcg gcgcgctcct tgcgctgctg ctctgcctcg gcgccgcctc cgcccgtgag
      541 gtgaagttga cggcggatac catgcagtac gattccgcaa gcgggtactt tacggcgacc
      601 gaaaatgtga agatcgtccg ggagggcgtc actgcgaccg ccaaaaaagc cgaggggaac
      661 atgaacggcg gagaagtcaa tcttatcggc gatgttcata tcttcggagt gtgggaagac
      721 gaaaaagtcg atataaaggg gcagatcctg accggatacc tccaggatcc gcgcggtctt
      781 tcctttaaag gaaccgtgca gggcgtctgg gggagcaaac aggtggacgc cgaggatctg
      841 agcacgcgcg gggatacgtt ctccgtagcg aaacttcgcc gtttcgccga ttccgccgag
      901 gggttctcct tttcgtgcaa ctcgatcaag gggaagctca aagacgggga gatcgtcgag
      961 tttaccgccg tcggcgcctt cagattcgtc tcgtcccaaa aaaaaggaac gccgacggag
     1021 atccgcggcg acagggcggt ctactccaag gcaagggggt cgctcgtggt gaacgggaac
     1081 gtctcggccg tccaggacgg gcgttcgctc cgctccgatt cgctggtcta ctttcctgta
     1141 aagaaccgca tcgaagccac ggggaagccg cagctgatct tcaaaataga cgaaggttca
     1201 aagaaacaat gagcaaaatg ctctcggctc gcggcctgtc caaagcctat aaaggcagga
     1261 cggtcgtctc ctcggcagac ctggacgtcg caatgggcga agtggtggga ctgctcggac
     1321 ctaacggggc cggaaagacg acgtcgttct atatgattgt cgggcttatc aaaccggacg
     1381 ccggagtggt caccgtggat tcgcgggatc tgacggatct tcccgtctac cggcgggctc
     1441 gtctcggcgt ggggtacctt ccccaggagg cgtcggtttt tcgcaatctt tccgtccgtg
     1501 aaaacatcga actcgtcctc caggaaagtc cgccccccgg agaaaatccg tcgattgtag
     1561 ccgaccgcct gatcgaagat ttcggcctgg agaagatcgc ccatgtccgg ggctactccc
     1621 tcagcggcgg cgaacggcgc agggtggaga tcgcccgcag cctcgccatt ctgccgaact
     1681 ttctccttct cgacgagccc ttcagcggta tcgaccccat cgccgtctac gatatacagc
     1741 agatcatcct cggcctccgc gcgaaggggt acggtattct cctgacggat cacaacgtcc
     1801 gcgacaccct tgccatcacg gacaggacgt accttataca ccaggggaag atcgtcatcg
     1861 aaggctctcc cgatgaagtg gcggagagcg aggttgcgcg gaagttctac ctgggagagc
     1921 agttctcctg gtaggcttcc acctcgtccc accgtccttc caggaggagg ggagcgagcg
     1981 tctcgacggc cagggaaact tccggtgaaa gcgaatcgcc gaaacctctc cgggcgggct
     2041 gaatccccag gacggtcact tcgagctccg gaagcagcgg tgcgaggagc agcggcagcg
     2101 gtattccggc gtcgccttcg gaacgcacgg aataccgctg ccgctgcatc ctccgtacgg
     2161 ctcctccggg aagccccatc tccgccgcgt cgacgatgag aagatgcacg ctgtcgttcc
     2221 gtatggcccg cgtcagcgga gcgatataat tttcgggcgt cgtttcgcag ttcacgacgc
     2281 gaagctgtcc gggggcaacg ccctccaaga tggtgaaaag tgcgggtcct gctccgtcgt
     2341 cctgaagaag cgggttcccg acgcaccaca gtacggtgtg ccgcattcgc gcattcgctc
     2401 ctttcgcaaa aagaacttat acagttgaaa caaggaggcc ggttccgtgg ccaagtcaac
     2461 gcatacgatg gtacgccacg ctttgtcgat gatgctgggg acgttcgcga gcagggtgct
     2521 cggtcttgtc cgggagatca tcacggcggc gtggttcggc gcgtcggggg tgctcgacgc
     2581 gttcaacgtc tccttcacgc tcgcgaacct ggcccgccag cttttggcgg agggagcgct
     2641 ttcagcctcc ttcgtcccgg tcttctcaag ggttctggcg gccaagggaa aagaaagcgc
     2701 ggagcgcctc gcgcgtcagg ccttttccgt gctgctcgtc gctacgattc tctccgtcgc
     2761 ggcgggcgtt gtgttttccc cgctgctcgt aaagattatg gctccggggt ttgatcctgt
     2821 caaagccgaa ttggcgacgg cgatgacgcg ctggatgttt cccttccttg tcctggtgtc
     2881 gcttgcggcg ctcgcgatgg gggtgctcaa cagcatgggc tcctttctcg ttcccgcgct
     2941 tgccccggcg ttgagcaatc tcgtctacat cgtgctggtt gtgttccttg cctctttcta
     3001 cggtgtttgg ggcctcgtga tcgcggtgct cgccggtggc gcctgccagt ttcttctcca
     3061 atgggcgtgg tcggtacgga tgggggttac gctgctcccc gagtggccgc aactgaagga
     3121 tccagatctc cggacgatgc tcgccctctt ccttccgtac gccgccggac tctcgctgaa
     3181 ccaggtgaat ccggtgatca gcaggatgct cgcctccttt ctccaggagg gcgccatctc
     3241 tgtgctgaac tacgcaaacc gcgtgatcca gctccccctg ggaattttcg tcatcgcgat
     3301 atcgcaggcc gtcctgcccc aactctcccg atgcccggcg gaagataccg gggagttccg
     3361 cgacatcatg cgcgactcgc tcaggttcac gctcttcgtc gtctttcccg cgacgctggg
     3421 cctggcgctt gtctcggacg aaatcgtcca tctgctcttc gtcaggggag cgttcggcga
     3481 gtgggcctgg aaagggacct cggtttcact cgcgatgtac tctctcgggc ttcccggaat
     3541 ggcgttctcc acggtggtca tgcgggggct ctacgcccga agcctgccgc gcggggcgct
     3601 tatcgtcact ctctccagcg tggcggccac gctgctcttt tcgcttgcgc tgatggggcc
     3661 gctctcgttc ggaggcctcg cgctcgcaac gtcgctggcg ttcagcctct ccgggtgggt
     3721 gggcgtccgc atactttccc gccacttagg gatgcggttc ggtatcttct ccgtcccctg
     3781 gacggcaaag atgggcgtgt cgctggcttt tacggggctg atcgtcgtcg cgtggcgatg
     3841 gtttttcccg tattcgccgg agtcttcagg gattctcagg gctgcctggg tcggcggcgt
     3901 cttttgcgca gctgcgtcgg gatatgccgc cgtgaccgct ctgcttcgct tccaggagtg
     3961 gcgctggctt ttcggcgccc tccggaggaa ggaccggcgg gagacgccgg gacagaatgc
     4021 aaaggaggaa taaagtatga cgtttcgtaa gctgcttctc tgtgtgtgtg tactcgcgct
     4081 gctccttccg gtacagtcgt ttgcgggaga tgtggagtcg ctcctcgaaa agagaagcgc
     4141 gattctctgg ttcgagggcg aagagttcgg tgaactcatt attggcgcca gggcgcagtt
     4201 cgccttcgtc tacgtcgacg gcgcgctctc agaggccgtg gtgaaagagc cgggcgctcc
     4261 cgactggctg cgggacaaca tctactacta cgggtcgaag gagacgcgga agaaagcgct
     4321 cttcatcgtc cggctccgaa cggtgaagaa tcttgatctt cagctctcga tgatcaagat
     4381 cggcacgcac acgctcacgc cggaggatgt gctcacgaac aaacactatg tgccggtagg
     4441 ggagctgcct cccgatttct cggcggcctt cgctgtcgcc gtgcccctcg ccgcggtgaa
     4501 agggacaacg cggactgtct ccgtcggcga ctacggcacg gagctggagt tcccgatacg
     4561 atgagcctcc cggaagagtc ttttcttact ctcggaatgg agagcagctg cgacgacacg
     4621 gcggtcgccg tccttcgcgg cggacggcgg gtggaggcgg agctgctttc cagccagctt
     4681 gaggaccacg ttccgttcgg aggggtcatc cccgaattcg cggcgcggcg gcacctggaa
     4741 gcctttcttc ctctcacgga ggaggcgctc cgcagggcgg gcgtcgccga cccccggcgg
     4801 gagatagggc tcgtcgccgt cactgcgggg ccgggactca tggggtcgct gctcgtcggc
     4861 gtcatggcgg cgaaagcgct ctcccttgct tgggacgtcc cgctcctcgg tgtgaatcac
     4921 cttgaagggc atatcttcgc caatgtcgtc tcgcacccgg agctctcccc tccctttctc
     4981 tgcatggttg tctcgggggg gcacacggag atcgtccttg tcaagaagtt cgggacgtat
     5041 attttcctcg gcggaacccg cgacgacgcg gcgggggaag cgtacgacaa ggtcgcgaag
     5101 gtcctcggcc tcccgtaccc cggcggaccc gtggtggaca agcttgccgc ctcgggaaat
     5161 cccgacgctt tcgactttcc ctcgccgctc aagggaagcc cggagatcgt cttcagcttc
     5221 agcggactca agactgcggt gctcacccgg ataaagaagc tacggaacga agggatcgag
     5281 attcccgtcg aggacgtctg cgcctctttc cagagggccg tgaccgactc attgatgacg
     5341 aagctgcgcc tcgcctcgcg gcggacggga gttcgcgccg tctccatctc gggcggcgtt
     5401 gctgcgaaca gcgcgctgag gaatgcgctt cttcaggaaa aagggtggaa ggtcttcctt
     5461 ccgccgaaaa accgctgtac cgacaacgcc gtcatgatag ccgcggcagg ctattccgcc
     5521 ttccggagag gtgcgcgctc ttcacttgat ctggcgccgg acccgtcgtg ggagctttgg
     5581 aaaaagtagt tcttccggaa tatgtcaata tgccttgaaa aatgcctata tctagctgtt
     5641 tgaaggccat aatcgagaat atttatcgct caccgctctt gagaatcctt cccttcatac
     5701 gtaatatcta tcccggacaa tttagcactc aacaatatcg agtgctagca ttttgtaaaa
     5761 acacacattt gggagggatt tgtaccatga acctgaaacc gcttggagac agactggtag
     5821 ttaaggtact gcataacgag gagaagacca agggcggcat cgtacttccg gataccgcga
     5881 aagaaaagcc caccgagggc gaagtcatcg ctgtcggcac cggcaaggtg cttgagaacg
     5941 gccagaagct tccgcttgaa gtcaaggtcg gcgaccgcat catcttcagc aagtacgcgg
     6001 gtaccgaagt gaagctcgac ggcgacgagt acgttatttt cagcgagcgc gacgtgcttg
     6061 cgattatcga taagtagtct gaattcctga ggaggtaaaa cgattatggc taaaattctt
     6121 attttcggag aagaagcacg ccgcgcgatg cttcgcggta ttgataaagt tgccgacacc
     6181 gttggcgtaa ccctcggccc caagggccgc aacgtggttc ttgagaagaa gttcggttcg
     6241 cccaccatca ccaacgacgg cgtgaccatc gccaaggaga tcgagctcga ggacgtgtac
     6301 gagaacaccg gcgcccagct tctgaaggaa gtcgcctcca agaccaacga catcgcggga
     6361 gacggcacca ccaccgcaac cgtctttgcc cgcgcgatca tcgccgaggg catgaagaac
     6421 gtcgcggccg gcgccaacgg catgcagatg cgttccggca tcgagagagc catcgacctt
     6481 gtcgtcgacg agctgaagaa gaaggctatc tccgtcaaga accgcgacaa gatcgctcag
     6541 gtcgccgcca tttccgcgaa cgacaaagcg gtcggccagc tcatcgccga ggccatggac
     6601 a
//
LOCUS       DAOPKU010000119         6514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12611_length_6514_cov_4.569748, whole genome shotgun sequence.
ACCESSION   DAOPKU010000119 DAOPKU010000000
VERSION     DAOPKU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12611_length_6514_cov_4.569748"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="PK849_05130"
     CDS             <1..1370
                     /locus_tag="PK849_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPE65541.1"
                     /translation="PSVLRLWAGALILAASAVLAGRVYCSVLCPLGTLQELIWRTGKR
                     SASYRKPSQLRYWVLGGVVVAAAAGYMFPAAFLDPYAAFGRGVTHLLRPLAIGGNNMI
                     ATFLEERGVYGLLYRAGRSPLLPGVLWGTTLFFALLGFWSFFRGRPFCDALCPVGTFL
                     GLFSASPVLGVRFDGERCVACGRCESVCPEGCISVVNRRVETDRCILCLRCLDACPAG
                     GLKLGRLSPREGLVRRGALRFGLGALAALGAALRARLPDRTVSASASSREERLPISPP
                     GSGSHGNFTKKCVTCTACVTACPAGIIRPSLSAWGMQGIFQPELDYSKGYCQFECAAC
                     SSACPTGAIAPLTLDEKKRTSIGRARFFRRNCIVRKYGQACGACSEHCPTQAVRMVPF
                     RRNLTIPEVDPTLCLGCGACEYACPAEPKAITVSGVSVHGKIRDRKNEGAEAPVVAPL
                     EEFPF"
     gene            1474..1665
                     /gene="tatA"
                     /locus_tag="PK849_05135"
     CDS             1474..1665
                     /gene="tatA"
                     /locus_tag="PK849_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPE65542.1"
                     /translation="MNLGMGEILVLLALALVLFGAKRLPEVGRAAGSAIREFKRGLES
                     GEAPEKAGEDTGETNEDGK"
     gene            1640..2359
                     /gene="tatC"
                     /locus_tag="PK849_05140"
     CDS             1640..2359
                     /gene="tatC"
                     /locus_tag="PK849_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPE65543.1"
                     /translation="MKRTKTGSEGEFSDHLEVLRRKIIAVLFFFCCATALLFLLSERW
                     VRFLQAPLEGLGVSLYYFKPYEKFLTYMRLSFWGGAALSVPLAVLQAALFVAPALRKN
                     EMKYLILAGGLIPALFLAGAAFAYRFAAPLALRFFLFFGEGDNVLPLWGFGDYASFLF
                     SLLLASGMLFQAPLLLLLFILFGLVSVETLSRLRPWIILGIALIAALLTPPDVVSQIL
                     LGVPLYLLFELVLVLGRFLKR"
     gene            complement(2362..3288)
                     /locus_tag="PK849_05145"
     CDS             complement(2362..3288)
                     /locus_tag="PK849_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPE65544.1"
                     /translation="MSARTTSPLLYFSAYAALYIIWGSTYLAIRFTVETIPPFLSGSL
                     RFFLAGGILLVWCLYREQERPTLRGFLAALKVAGIMLLGGYGGVVWAQQTVPSSMAAL
                     IISIEPLWFVIFDWLFFKSRKPSTAETIGVALGFGGTVFLVLSQSGYSLSLKGEYTVG
                     MLVVLVATFNWSMGALYSRKAPIAKSSLLAAGMQMTAGGCLLLLVSALTGEPSSLTMD
                     AVSLKSMLALGYLVVFGSLIGFTAFIWLLKVDRASRVITHTFVNPVVAIFLGWFLGDE
                     TITLPMLGAAVVIIFSVVLITRYHPDESAASA"
     gene            3620..6055
                     /locus_tag="PK849_05150"
     CDS             3620..6055
                     /locus_tag="PK849_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPE65545.1"
                     /translation="MLPKKMVDLDRVIASSPVVVLWGEPLDHRPVDFVTDNVSQFGYA
                     VPEMVLEEVRYDSIVFDEDRERVRSAFQNALLDGSDSFILDYRIITRAGAVRWVEDRV
                     AFHRDETGKVCLYQSSLLDITERKTAELQLRESEERLARVLVSARVGVWEYLPESDEL
                     LGDGRASLFGISDDAIPKSRKELWDAIVPPAGRERLENAWREILEGTTDHLDLEHKLI
                     KVDGSAVWVLTKGFPVRGEDGGLLRMQGVTVDISPLKQAEEKVVQQNERLALLHEISL
                     AFMEELDSESLIRRILEKAVELAGSANGLIGVIEEDGENIRNTWGVGLYEKLVGNLVP
                     VSVGLIGEVLKKKSRVIVEDYRTYPERMELDAYTNVTTVLGLPLYRGERLLGAVAIAF
                     TDTVRFLEEPLLNLLDQFAAAASIALENARFYEDARKEVEERERTEQRLRFHQALVEA
                     AAEGASFLLSIESEEQAMFFALRALGQAAAADSIALFRNSESQEGAVAKIIAKAPISG
                     NEEFFILPDHLPWGDEYSSIYESLSDGMPFHGTIASIASERHDASFATITMIPVFIRS
                     YFWGVLAFCFQRERPPFETDEIDVLRAAAYNMAASVIRWESEREVKKGYEKLRRTFTD
                     VIRTMGQIVGKKDPYTIEHQERVAALALEIGNRLGLGKERCEGLRIAGLVHDIGKIEI
                     PGEILSKPGRLSPIEFELIKTHARSGYDILREVDFPWPVAEIAHQHHERMNGSGYPRG
                     LKGEEILLEARIITVADVVESMVSHRPYRPSLGINAAREEIAEKSGVLYDSAVVDACL
                     SLLDERPDLIAEL"
     gene            6080..>6514
                     /locus_tag="PK849_05155"
     CDS             6080..>6514
                     /locus_tag="PK849_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPE65546.1"
                     /translation="MMPKDTVSLDQVFISSPVVVLWGAPLEGRPVEFVTKNVSQFGYA
                     AGDLMTGSVTFGAILHEEDRERCFTLFSDALFGREDEIRMEYRIVAPNGDVRWVEDRT
                     VFHREPAGEAVAYQSSLLDITGRKLSEMRLREAEVRLARVLEA"
BASE COUNT         1283 a         1755 c         2068 g         1408 t
ORIGIN      
        1 tcccgtccgt acttcgattg tgggccggag cccttatcct tgctgcaagc gccgtcctgg
       61 cggggcgcgt gtactgctcc gtcctctgtc cgttgggaac gcttcaggag ctgatttgga
      121 gaacgggaaa gcggagtgcg tcctacagaa aaccatcgca gctccgctac tgggtgcttg
      181 gaggagttgt ggttgcggct gcggctgggt acatgttccc ggcggcgttc ctcgacccct
      241 atgccgcgtt cggcagggga gtcacgcatt tgctccgacc tcttgcaatc ggtggaaaca
      301 atatgatcgc cacgttcctc gaagagcggg gcgtctacgg cctgctttac agagccggcc
      361 gaagcccgct gcttcccggg gtgctgtggg ggacgacgct ctttttcgcg ctccttgggt
      421 tctggtcgtt tttcagggga agaccgttct gcgatgcgct ctgccccgtc gggaccttcc
      481 tgggtttgtt ttcggcctcg cctgtcctcg gcgtacgttt tgacggtgaa cggtgcgtcg
      541 cgtgcggaag gtgcgagtcc gtctgtcccg aagggtgcat ttcggtagta aaccgccgtg
      601 tggagacaga ccgctgtata ctctgtcttc gctgtcttga tgcgtgccct gctggagggc
      661 tgaagctggg gcggctctcc ccgcgcgagg ggcttgtccg gagaggggcg ctccgtttcg
      721 gtctcggcgc gctcgcagcg ctcggcgcgg ccttacgcgc caggctgccc gacagaactg
      781 tttccgcgtc ggcgtcgtcc cgggaagagc gcctgccgat atcgccgccc ggctcgggga
      841 gccacggcaa cttcacgaaa aagtgtgtga cgtgcactgc gtgcgttacg gcctgtcctg
      901 cgggaatcat ccgtccttca ctctccgcat gggggatgca aggcattttt cagccggaat
      961 tggactattc aaaggggtat tgtcagtttg aatgcgcggc ctgctcttct gcatgcccaa
     1021 cgggcgcgat tgccccgctg acgcttgatg aaaagaaaag aacgtctatc ggaagagcgc
     1081 gttttttccg gcgcaactgc atcgtcagga agtacggcca ggcgtgcggc gcgtgctccg
     1141 agcactgccc gacgcaggct gtccggatgg ttccgttccg ccgcaacctg accattcccg
     1201 aggtggatcc gacgctgtgt cttggctgcg gcgcgtgcga atacgcctgc ccggccgagc
     1261 cgaaggcgat caccgtttcc ggcgtctcgg tccacgggaa gatccgggac cggaagaacg
     1321 agggggcgga agctcctgtg gtggccccgc tggaggagtt cccgttctga accttaggga
     1381 aagtacgagc gatcacgcgc gggagcgctc cgccggaaac gctgagccgc gctcttgcgc
     1441 cgagaaagga cgcagagaag gaggggtgtt ggcatgaacc tgggaatggg agaaattctg
     1501 gtacttctcg cgcttgccct ggtgctcttc ggagcgaagc gccttcccga ggtcgggagg
     1561 gcggcgggaa gcgcgatcag ggagttcaag agagggcttg agtcgggaga agcgccggaa
     1621 aaggcgggag aagatacggg tgaaacgaac gaagacggga agtgaggggg agttttccga
     1681 tcaccttgaa gtcctccggc gcaagattat cgccgtcctc tttttcttct gttgcgcgac
     1741 ggcgctgctc tttcttctct ccgaacggtg ggtgcgcttc ctccaggctc cgctggaggg
     1801 gctcggagta tctctctact atttcaagcc ctacgagaag tttctgacgt acatgcgtct
     1861 ttccttctgg ggcggcgcgg ccctgagcgt gcctcttgcc gtccttcagg ccgcgctctt
     1921 cgtcgcgccc gcgctccgca agaacgagat gaagtacctc attcttgcag gcggtcttat
     1981 cccggcgctc tttctcgcag gcgccgcgtt cgcgtaccgg tttgcggcgc cgctcgccct
     2041 ccgtttcttc ctcttcttcg gcgagggaga caacgtgctt cccctctggg gattcggcga
     2101 ctacgcgtcc tttctgttct cgctgctgct ggcgtccggc atgctctttc aggcgccgct
     2161 ccttctgctg ctctttattc tgtttggtct tgtctccgtg gagacgctct ccaggctacg
     2221 gccgtggatc attttaggaa ttgcgctcat cgcggcgctg cttactccgc ccgacgttgt
     2281 gtcgcagata ctcctcggag ttccgctcta ccttctcttc gagctggtcc ttgtcctggg
     2341 acgttttttg aagcgttgaa tttacgctga cgccgcgctc tcatccggat ggtagcgtgt
     2401 gatgagcacc acggagaaga tgatgacgac cgccgcgccg agcatcggaa gcgtgatcgt
     2461 ttcatctccg aggaaccatc cgagaaaaat ggcgacgaca gggttgacga aagtgtgggt
     2521 aatcacccgt gacgccctgt ccaccttgag cagccagatg aacgcggtga agccgatgag
     2581 cgaaccgaag acgacaaggt agccgagcgc gagcatggat ttgagcgata cagcgtccat
     2641 agtgaggctg gaaggctccc cggtgagcgc ggagacaagg agaagcaggc agcctccggc
     2701 tgtcatctgc attcccgcgg caagcaggct ggacttcgcg atcggggcct tgcgcgagta
     2761 gagcgctccc atgctccagt tgaatgtggc gacgaggacg accagcatac cgacggtgta
     2821 ctctcccttc agcgagagcg agtaccccga ctgcgagagg acgagaaaga cggtgccgcc
     2881 gaatccgagg gcgactccga tcgtttcggc cgtagacggc ttgcgcgatt tgaagaagag
     2941 ccagtcgaag atgacgaacc agagcggttc gatggagatg atgagggccg ccatggacga
     3001 gggaactgtc tgctgcgccc ataccacgcc gccgtaccct ccgagcagca tgatcccggc
     3061 aactttcaat gcggcgagga atcctcgcag cgtgggacgc tcctgttccc tgtacagaca
     3121 ccagacgagc agaatgcccc cggcgaggaa gaagcggaga gagccgctga ggaacggcgg
     3181 tatcgtttcc accgtaaagc ggatggccag atacgtggaa ccccagatga tgtaaagcgc
     3241 cgcgtaggcg gagaagtaga gcagcggaga cgtggtgcga gcggacatga caataacccc
     3301 cagaatgtat tcgaatcgcg gcgatattat agcagatagt tccttcttac aacatcctcg
     3361 attgaaaaaa gcagattttt aagagggaat tgccggtccg gtgtttctcc tccgttccaa
     3421 cctgcttccc ggcggcgcgc tcttcagcgg cgagcgcgca ggactgcgct tttcgcccgc
     3481 gaatttacgg atggaatcct cctgttcaaa gcggaagaat tcgcgtaaaa taaaaaatgc
     3541 aatgtgataa gttgattctt tgtccgcatg cgtttttgtg ctgttgacct gttttttcca
     3601 agagcgaagg agatggagca tgcttccgaa gaagatggtc gatcttgatc gggtgatcgc
     3661 atcaagcccg gtggtggtcc tctggggcga gccgctggat catcgtcccg tggacttcgt
     3721 aacggacaac gtgagccagt tcggatacgc tgtgcccgag atggtgttgg aggaagtccg
     3781 atatgactcc attgtgttcg acgaggacag agaacgtgtg cgttcagcct ttcaaaacgc
     3841 attgctggac gggagcgact ccttcatcct cgactataga atcatcaccc gcgccggagc
     3901 ggtccgctgg gttgaggacc gggttgcatt ccaccgcgac gaaacaggga aagtatgtct
     3961 ctatcaatca agccttctgg atatcacgga aagaaaaacg gcggagcttc agcttcgcga
     4021 gagcgaggaa cgtcttgccc gcgtgcttgt ctccgccagg gtcggcgtgt gggagtatct
     4081 tcccgaaagc gatgaactgc ttggggacgg gagagcctct ctcttcggca tcagcgatga
     4141 tgcgattccg aagtcccgga aagagttatg ggacgccatt gttccgcccg ccggacgcga
     4201 gcggcttgaa aacgcatgga gggagatcct ggaggggaca acggatcatc tcgacctgga
     4261 gcacaagctc atcaaggtcg acggctcggc agtatgggtt ctgacgaagg ggtttcctgt
     4321 caggggtgaa gacggaggac tgcttcgcat gcaaggggtt accgttgaca tctctcctct
     4381 caaacaggcg gaggagaagg tcgttcagca aaacgaacgg cttgctcttc tgcacgaaat
     4441 ttcgctggca ttcatggagg aactggattc ggagtcgctt atccggagga ttctcgaaaa
     4501 ggccgtggaa ctcgcaggtt cggcgaacgg tcttatcggc gttatagaag aagacgggga
     4561 aaatatacga aatacctggg gcgtcggtct ttacgagaag ttagtaggaa acctggttcc
     4621 ggtttcagtc ggcctgatcg gcgaagtcct gaaaaaaaag agcagggtga ttgtagaaga
     4681 ttaccggacg tatccggaac gaatggaact ggatgcgtac acgaatgtaa cgacggtgct
     4741 tggtctcccc ctgtatcggg gagagcgatt gctgggagct gttgcaatag cttttaccga
     4801 tacggttcga tttctggagg aacccctgct gaatctcctg gaccagtttg cggccgctgc
     4861 ctccatagcg ctggagaatg ccagattcta tgaggatgcc cgaaaggaag tggaggagcg
     4921 ggaacgaacc gagcaacggc tgcgttttca ccaggcgctt gtggaagcgg cagcggaggg
     4981 ggcaagcttt cttctgtcca tcgaatccga ggaacaggca atgttcttcg ccctgcgcgc
     5041 tctcggccag gccgcggctg ccgacagcat cgcacttttc cgcaatagcg aaagtcagga
     5101 gggcgctgtg gcgaaaatta tagccaaagc gccgatttca gggaacgaag agttcttcat
     5161 tttaccggat catcttccct ggggggatga atattcttcc atttatgaat ctctttcgga
     5221 tggaatgcct tttcacggaa cgatcgcttc cattgcttcc gagcggcatg acgcttcttt
     5281 tgctacgatc acaatgattc ccgtgttcat ccgttcgtat ttctggggag tgcttgcgtt
     5341 ctgtttccag cgggagcggc cgcctttcga gacggacgag atcgacgtgc ttcgcgcggc
     5401 cgcgtataac atggctgcat cggtgattcg ctgggagtcc gaacgagagg tgaagaaggg
     5461 gtatgaaaaa ctccggcgga cgtttacgga cgtcatccgg acgatgggac agattgtcgg
     5521 gaagaaggac ccctatacta tcgagcatca ggagcgcgtc gccgcgctcg ccctggagat
     5581 cgggaacaga ttgggactgg gaaaggagcg ctgcgaagga ttacgcatcg ccggattggt
     5641 gcacgatatc ggcaagatcg aaataccggg ggagatttta agcaagcccg gaagactctc
     5701 ccctatagag tttgaactga tcaagacgca cgcgcgttcc gggtacgaca ttctgcggga
     5761 ggtggacttc ccctggcctg tagccgagat cgcgcatcag catcacgaga ggatgaacgg
     5821 atcggggtac ccgagagggc tgaaaggcga ggagattctt ctggaggctc gtatcatcac
     5881 cgtcgccgat gttgtggagt ccatggtgtc gcatcgtccg taccgcccct ctcttggaat
     5941 caacgccgcc cgggaagaaa tagcggaaaa aagcggcgtc ctgtacgatt ccgccgttgt
     6001 cgacgcatgt ctgtcgcttc tcgacgaacg tcctgatctc atcgccgaac tgtagcagcg
     6061 catgagcgga cgaactgcca tgatgccgaa ggataccgtc agtctcgatc aagtcttcat
     6121 ctccagcccg gttgttgttc tgtggggcgc tccgctggaa gggcgtcccg tcgaattcgt
     6181 gacgaagaat gtctcccagt tcgggtatgc ggccggggat ttgatgaccg ggagcgtgac
     6241 gttcggcgcc attcttcacg aggaggaccg ggaacgttgc ttcacgctgt tttccgacgc
     6301 tcttttcggg cgggaagacg agatccggat ggaataccgt atcgttgcgc cgaacggaga
     6361 tgtgcgttgg gtagaggacc ggactgtttt tcaccgtgag ccggcgggag aggctgttgc
     6421 gtatcagtcg agccttctgg acatcacagg gagaaaacta tcggagatgc gcctcaggga
     6481 ggccgaggtc cgtttggccc gcgtcctgga ggcc
//
LOCUS       DAOPKU010000120         6464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12764_length_6464_cov_5.034171, whole genome shotgun sequence.
ACCESSION   DAOPKU010000120 DAOPKU010000000
VERSION     DAOPKU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12764_length_6464_cov_5.034171"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK849_05160"
     CDS             complement(<1..290)
                     /locus_tag="PK849_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPE65547.1"
                     /translation="MWWEEGLRFTCLGCGRCCRGEPGAIFFTPEEEEKMATFLSLPVS
                     EFRRRYVTSRWRAPSLKEKNNGDCVFYDAAADRCFIYRVRPLQCRLFPFWPAL"
     gene            complement(291..656)
                     /locus_tag="PK849_05165"
     CDS             complement(291..656)
                     /locus_tag="PK849_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65548.1"
                     /translation="MQIDPRFASFVPSNRGIAESSVALKGTKEREKLKEACQQFESIL
                     LAEIWKKMNANARSIGGKSDSDRPFGPMEDLAVEMSAEQLAKEGGAGMWRMLYDQLVV
                     HLDSAEELARRRRPEEASE"
     gene            complement(657..1760)
                     /locus_tag="PK849_05170"
     CDS             complement(657..1760)
                     /locus_tag="PK849_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HPE65549.1"
                     /translation="MKRYGVLPLALLLLFITACGAAASHPDVRIKDLADVEGVRSNQL
                     VGLGLVMGLQGTGDRSAMATQMLRNIMSQFGISVNEKAVRSRNVAVVTLTAELPAFAR
                     PGQTIDVTASAMGDAKSLQGGTLLQAPLRAADGNVYAVAQGPLMVGGFAVEGAAGAVT
                     KNIVTVGRIPGGAIVERNVQTTFSAGRQINLLLRNPDFTTSERMAAAINSAYGGIASP
                     VDAGRVVVNVPPQYASSPAAFLAHIEGISLTPDTAARVAVNERTGTVVMGGNVRIGAV
                     AVAHGNLTVRVAENPEVVQPQPFGGGTTAVQPRTDIGINESPGQFVALDSTSTVQELV
                     DALNSVGASPRDVIAILQAIKEAGALHGELVVM"
     gene            complement(1774..2352)
                     /locus_tag="PK849_05175"
     CDS             complement(1774..2352)
                     /locus_tag="PK849_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HPE65550.1"
                     /translation="MKRSACVLILLLCASVLTGCAAEAASLWNDNTNWIADRRPSQVG
                     DIVTVFVDEKTKTKDEGTTEASKTNDNNVDDGFGIFDFIRAFGFSSSSKMTGDGSTER
                     THSAKGQITCLVTDVLPNGNLVIEGTRDIATHEETLQLHVIGVIRPQDVDSYNKIRSD
                     LVANAEIDIKGKGTISRLQKPGILTQILQAIF"
     gene            complement(2356..3243)
                     /gene="flgA"
                     /locus_tag="PK849_05180"
     CDS             complement(2356..3243)
                     /gene="flgA"
                     /locus_tag="PK849_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HPE65551.1"
                     /translation="MQPLRLSCTLLLFLSLLFALPSAASAVDLIIEMDAVVSVTGNPF
                     PLSEAARLSGDPALVQKTGALLFDIPPDGLLTRDTLLNTLVAKGIGGIRLKLVMPPEM
                     RIVLDESLVAIVKRLSGWRWTIDVEPLGTVPHGRLVSPLSIPPGTGSATLRFIDDRGN
                     ERALAVRLSWSQPAVVTTRPLERGAVIAAADVTVRDVRVMRSQPLASSPEEVIGRSLG
                     RNLTAGEPVLLNSLSRTPIIQRGDSVIITVRKPGFLVEVRGEALDAGAEGESIRVRNL
                     QSRAVIQAVVVGSGRVEVQ"
     gene            complement(3287..4075)
                     /gene="flgG"
                     /locus_tag="PK849_05185"
     CDS             complement(3287..4075)
                     /gene="flgG"
                     /locus_tag="PK849_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HPE65552.1"
                     /translation="MLRALWTSASGMIAQQTNLDVTTNNLANVNTSGFKKKRADFADL
                     LYQINREPGAPVEPASMVPTGVQVGLGVRVVGTPRIMTQGNLQVTDRPLDITIEGDGF
                     FQVLQPNGEVAYTRAGGWNLDGEGQVVNTDGLLIEPAILVPEDATEIMISPEGLVSVK
                     IDGQPEPEEIGQIELARFVNSGGLRAIGKNLFLETAASGAPIVGNPGDEGLGQVHQGI
                     LEMSNVQVVDEMVNLIIAQRAYEANSKGVQTADELLRIANGLKR"
     gene            complement(4092..4901)
                     /locus_tag="PK849_05190"
     CDS             complement(4092..4901)
                     /locus_tag="PK849_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HPE65553.1"
                     /translation="MHRGLYAAASAMIVQESMHDVVANNLANVNTSGFRRRIPVNKSF
                     PEVLMDRIEKKSEDGEIKLIAPPFQLGLKGKFLIGDLSLANVISETYMTTESGAIQVT
                     DNPFDAAIVGEGYFAVQDGAGNIYYTRSGHFQKNGEGQLVTEDGMLVLGDGGPIEVGD
                     ASRFAITENGNVIADGAVVDMFQVVTFENPTYLRQVGRTALAVTPQSGAPVPVEAIRL
                     EPGVLEMSNVNVVEEMVRMVEAHRAYESASKVLMTHDELTGKLISSYGRTS"
     gene            complement(4956..6002)
                     /locus_tag="PK849_05195"
     CDS             complement(4956..6002)
                     /locus_tag="PK849_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPE65554.1"
                     /translation="MFGTDIGIDLGTATVLMYVKNKGIVLREPSVVAVDLDTGKILAV
                     GYEAKNMVGRTPGSITSVRPLKDGVIADYTMTEAMMHHFMRRVLKGFNRFFRNRVMIC
                     VPSGATDVERRAVLEAAVEVGAKEAYLIEEPMAAAIGANLNVEEPRGKMVVDIGGGTT
                     DIAVISLGGIVVSKSLRIGGDRFDEGIIRFLRKQYNLAIGEQTAENLKIMIGTCLQEA
                     PDTKMILKGRDLVQGLPRQIEVSSASVSMAIGEMVQALIDGVRNVLELTPPELSADII
                     DRGIVLTGGGALLRGLTELITRQTGINCFTADDPMECVALGTGKALAEIDKLQASGRS
                     GILVNRRKGRKKKL"
     gene            complement(6100..>6464)
                     /locus_tag="PK849_05200"
     CDS             complement(6100..>6464)
                     /locus_tag="PK849_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65555.1"
                     /translation="RTETLKACALYEGTHDFGAFCKTTERPDRSVRTVHRATLRKKGD
                     LTIFTVRGNAFLTNMVRIMVGNLQAVGYGKKDVEWLASLLSGGARAESAMTAPPEGLY
                     FWRVGYGEGTPFRTNGIE"
BASE COUNT         1173 a         1952 c         1950 g         1389 t
ORIGIN      
        1 agggcgggcc aaaaggggaa gagacggcac tggagcggcc ggacccggta gatgaagcac
       61 ctgtcggcgg ccgcgtcgta gaagacgcag tccccgttgt ttttctcctt cagactgggc
      121 gctctccatc tcgaggtgac ataccggcgg cgaaactcac tcaccggaag agagaggaat
      181 gttgccatct tttcctcctc ttccggcgtg aaaaagatcg ctcccggttc tccgcggcag
      241 cagcggccgc atccgagaca ggtgaaacgg agtccttcct cccaccacac ttattcggac
      301 gcctcttccg gacggcgtct tcttgcaagc tcctcggctg aatccagatg gacgacaagc
      361 tggtcgtaga gcatccgcca cattcctgcg ccgccctcct tcgccagctg ttccgcggac
      421 atttccacgg caaggtcctc cattggtccg aagggacggt cggagtcgct tttgccgccg
      481 atgctgcgcg cgttggcgtt catttttttc cagatctccg ccagaaggat ggattcgaac
      541 tgctggcagg cttctttcag tttttcccgt tctttcgttc ctttcaacgc tacggatgat
      601 tccgcaattc ccctgttgga gggaacaaaa gaagcaaaac gcggatcgat ctgcatttac
      661 atcaccacca gttccccgtg aagcgccccg gcctctttga tggcctggag aatcgcgatg
      721 acgtcccgcg gcgatgcgcc tacggaattc agcgcgtcca ccagctcctg cacagtgctt
      781 gtggagtcga gggcgacgaa ttgaccggga gattcgttga ttccgatgtc ggtacgaggc
      841 tgcacggcgg tcgttccccc gccgaacggc tgcggctgca cgacctcagg gttttccgcc
      901 acgcggaccg tgaggtttcc gtgagcgacg gcgacggcgc cgatacgcac gtttcctccc
      961 ataacgaccg tgccggtgcg ttcattgacc gcgacccgtg ccgcggtgtc gggggtgagc
     1021 gaaattcctt cgatatgggc gagaaaggct gccggcgacg atgcgtactg gggtgggaca
     1081 ttcacgacga ccctgcctgc gtccacaggg gaggcgatgc ctccgtaggc ggagttgatc
     1141 gccgcggcca tgcgctcgga ggtcgtaaag tcggggttgc ggagcagcag attgatctgc
     1201 cgtccggcgg agaatgtggt ctggacgttc cgttcgacga tggcgcctcc tggaattctg
     1261 ccgacggtga cgatgttctt ggtgactgct cccgccgctc cctcgacggc gaacccgccc
     1321 accatcaatg gcccctgggc cactgcgtag acgttgccgt ccgccgccct gagcggcgcc
     1381 tgaaggagcg tgcccccctg aaggctcttc gcatccccca tcgcgctggc tgttacgtcg
     1441 atcgtctgtc ccggacgagc gaacgcagga agctcggcgg tcagggtgac gaccgccacg
     1501 tttctgctgc gcacggcctt ttcgttcacc gagataccga actggctcat gatgttgcgg
     1561 agcatctgcg tggccatcgc gctgcggtcg cccgtcccct ggaggcccat gacaaggccg
     1621 aggccgacga gctgattcga acggaccccc tcgacatcgg cgaggtcctt gatgcggacg
     1681 tcggggtgag aggcggcggc gccgcaggcg gtgatgaaga gcaaaagaag tgcaagcgga
     1741 agaactccgt accgtttcat ggtccgcgcc tccttagaag atcgcctgaa ggatctgcgt
     1801 caggatgccc ggcttttgca gacgggagat ggtacccttc cccttgatgt cgatctcggc
     1861 gttcgcgaca aggtcgctcc tgatcttgtt gtagctgtcc acgtcctggg gccggataac
     1921 gccgatgaca tggagctgaa gcgtctcctc gtgggtggcg atgtctctcg ttccctcgat
     1981 aaccaggtta ccgttgggga ggacgtcggt gacaaggcag gtgatctgcc ccttggcgct
     2041 gtgggtccgc tcggtggaac cgtctccggt cattttgctc gacgagctga agccgaacgc
     2101 gcggatgaag tcgaagatgc cgaaaccgtc gtcgacgttg ttgtcgttcg tcttgctcgc
     2161 ctcggtcgtc ccttcgtcct tggtcttggt cttttcgtcg acaaaaacag tcactatatc
     2221 gcccacctgc gacggtcggc ggtcggctat ccagttcgta ttgtcgttcc acagcgacgc
     2281 agcctccgcc gcgcagcccg tgaggaccga cgcacacagc agaaggatga gaacgcacgc
     2341 gcttcgtttc attgcttact gcacctccac tcttccggat ccaacgacga ctgcctggat
     2401 cacggctctg ctttgcaggt tacgcacccg gatactctct ccttcggcgc ccgcatcaag
     2461 ggcttctcct ctgacctcga cgagaaaacc cggctttctt acggttatta taacagagtc
     2521 tcccctttga attatcggag tgcgagagag ggagttgaga agtacaggtt cgcccgccgt
     2581 aaggtttctt ccgaggctgc gcccgatcac ttcctccggt gacgaggcca gcggctgcga
     2641 acgcatcacg cgcacgtctc gaaccgtaac gtcggccgct gcgatgaccg ctcccctttc
     2701 gagggggcgc gttgtgacga cggccggctg actccaggag agtcgaacgg caagcgcccg
     2761 ttcattcccc cggtcgtcga taaaacgaag ggttgcagaa cccgttcccg gcggtatcga
     2821 aagcggagag acaaggcggc cgtgaggaac cgttccgaga ggttctacgt caatcgtcca
     2881 tcgccacccg gaaaggcgct tgacgatggc aaccagagat tcatcaagga caatacgcat
     2941 ctcgggaggc atgacgagct tgagtctgat gccgccgatg ccctttgcaa caagggtgtt
     3001 gagtaaggta tcgcgcgtca gaaggccgtc gggagggata tcgaaaagga gcgctccagt
     3061 cttctgcacc agggcaggat caccggagag gcgggccgcc tccgaaagag gaaaggggtt
     3121 gcccgtgacg gagacgacgg cgtccatctc tatgatgaga tcgacggcgg acgcggccga
     3181 aggaagagcg aaaagaagcg agaggaaaag aaggagggtg caggaaaggc ggaggggctg
     3241 cacaccgctc cgccttagga gattgtgaat gaaagacgcc actagcctac ctcttgaggc
     3301 cgttggctat tcggagcagt tcgtccgctg tctgcactcc cttggagttt gcttcgtacg
     3361 ctctctgtgc gatgatcagg ttgaccatct cgtcgaccac ctggacgttg gacatttcga
     3421 ggatgccctg atgcacctgt ccgagccctt catcacccgg gttgccgaca atgggcgcgc
     3481 cgctggccgc tgtttcgagg aagaggttct ttccgatggc gcgaagtcct cccgaattga
     3541 caaaccgggc gagctctatc tggccgatct cctccggctc cggctgtccg tctattttga
     3601 ccgagaccag cccttccggg ctgatcatga tctcggtagc gtcctcggga acaaggatcg
     3661 cgggctcgat gagcagtccg tccgtgttga cgacctgtcc ttccccgtcg agattccagc
     3721 cgcctgcgcg ggtataggcc acttcgccgt taggctgaag tacctgaaag aaaccgtccc
     3781 cctctatggt gatgtcgaga ggacggtccg tcacctggag gttcccctgc gtcatgatgc
     3841 ggggcgttcc aacgacccgg acgccgagtc caacctggac gccggtcggc accatcgacg
     3901 cgggttcgac gggtgcgccg ggctccctgt tgatctggta gagcagatcc gcgaagtcgg
     3961 cccgcttctt cttgaagccg gaagtattca cgttcgcaag gttgttggtg gtgacgtcga
     4021 gattggtctg ctgcgcgatc atcccgcttg cgcttgtcca taaggctctg agcatttctt
     4081 tctacctccg gttaactcgt cctgccgtag gacgagatca gttttccagt gagttcgtcg
     4141 tgcgtcatga gcactttgga ggccgactcg tacgcgcggt gcgcctcgac catccggacc
     4201 atctcctcga ccacgttcac gttggacatc tccaacaccc ccggttcaag gcgaattgct
     4261 tcgaccggaa cgggagcgcc ggactggggc gtgactgcaa gcgcggttcg ccctacctgc
     4321 ctgagatagg tggggttttc gaaggtcaca acctggaaca tgtcgaccac ggcgccgtcg
     4381 gcgattacat tgccgttttc ggtaatggcg aaacgggacg cgtcgcccac ttcgataggc
     4441 cccccgtctc cgaggacaag catcccgtcc tccgtgacaa gctgcccttc cccgttcttt
     4501 tgaaagtgcc cggatcgggt gtagtagatg tttccggcgc cgtcctgaac agcaaagtac
     4561 ccttcgccga cgatcgccgc gtcgaagggg ttatcggtga cttggattgc gccggactcg
     4621 gtggtcatgt aggtttccga gatcacgttg gcgagactga gatcgccgat gagaaacttc
     4681 cccttgagcc cgagctggaa aggcggggcg atgagtttga tctcgccgtc ctcggatttc
     4741 ttctcgattc ggtccataag aacctcggga aacgacttgt tgacgggaat ccgtctccgg
     4801 aaccccgaag tgttgacgtt cgcgaggtta ttggccacga catcgtgcat actctcctga
     4861 acgatcatcg cggatgcggc ggcatagagt ccccgatgca tattctttcc tccctcgatg
     4921 ctccgccctg tcggaagttg cttccgcgcg gcggatcaga gcttcttctt ccggcctttc
     4981 ctgcggttca ccaggatgcc gctccgcccc gaggcctgaa gcttgtcgat ctcggcgagg
     5041 gcctttcccg tgccgagcgc gacgcattcc atcgggtcgt cggcggtgaa gcagttgatg
     5101 ccggtctggc gggtgatcag ttcggtgagg ccccgcaaaa gagcgcctcc tccggtaagt
     5161 acaatccccc tgtctattat atcggctgag agttccggcg gcgttagctc caaaacattt
     5221 cgaactccat caatcagcgc ctgtaccatt tctccgatgg ccatcgagac gctcgcgctg
     5281 gagacttcta tctggcgagg aagcccctgg accaggtctc gccccttgag aatcatcttt
     5341 gtatcgggag cttcctgaag acaggtgccg atcatgatct tgaggttttc cgccgtctgt
     5401 tcgccgatgg cgaggttata ctgcttgcgc agaaaacgga taattccctc gtcgaagcgg
     5461 tcgcccccta tacgaagcga cttcgagacg acgatgcccc cgagggagat gacggcgata
     5521 tccgtagtgc cgcctccgat gtcgacgacc atttttccgc ggggctcctc gacgttgagg
     5581 tttgcgccga tcgccgcggc catgggttcc tcgatgaggt aggcttcttt agcgccgacc
     5641 tcgactgcgg cttcgaggac cgcgcggcgc tcgacatcgg tggcgccgga aggaacgcag
     5701 atcatgaccc ggttgcggaa gaagcggttg aagcccttga gcacacggcg catgaagtgg
     5761 tgcatcatgg cttcggtcat ggtgtagtcg gcgatgacgc cgtccttgag cggacggacg
     5821 gaggtgatgc tcccgggcgt tcgtccgacc atattcttgg cttcgtaccc caccgcgaga
     5881 attttcccag tgtcaaggtc taccgccacc acagagggtt cgcggagtac gatgcccttg
     5941 ttcttgacgt acataaggac tgttgcagtt cccaggtcta ttccgatatc cgttccaaac
     6001 acggtgtttt cctcctttgg atattgaaga ggctgttcgc gtccatctcg cggcaaggat
     6061 gcggatgttt cccgcaggtc cgtgccgtga acactctctc tattctatac cgttcgtgcg
     6121 aaatggggta ccctctccgt acccgacgcg ccaaaaataa agcccttccg ggggcgcagt
     6181 catggcggat tccgcccgcg ctcctccgga gagaagggag gcgagccatt ccacatcctt
     6241 ttttccgtac cccacggcct ggagattgcc caccatgatc cgcaccatgt tggtgaggaa
     6301 ggcgttgccg cggacggtga atatcgtaag atcccctttt ttgcggagcg tcgccctgtg
     6361 gaccgtacga acggaccggt ccggccgctc ggtcgtcttg caaaaagcgc cgaagtcgtg
     6421 cgtcccctcg tagagcgcgc atgccttcag cgtttcggtc cggt
//
LOCUS       DAOPKU010000121         6437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12844_length_6437_cov_4.369633, whole genome shotgun sequence.
ACCESSION   DAOPKU010000121 DAOPKU010000000
VERSION     DAOPKU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12844_length_6437_cov_4.369633"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PK849_05205"
     CDS             complement(<1..355)
                     /locus_tag="PK849_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65556.1"
                     /translation="MEKQLLTLEKLQNRILSLEEERLALSNALDIALKINDIQPDAAA
                     ETTVESILSVCYERTGRLIGLKEGGFYLFREEDAVFYQAFASSPAAKERIEHELQLLI
                     DDGTIAWVIQTERFVT"
     gene            complement(330..1598)
                     /locus_tag="PK849_05210"
     CDS             complement(330..1598)
                     /locus_tag="PK849_05210"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPE65557.1"
                     /translation="MGTLSITQLKSGMILSADLVAPNGRFILPKGACLTSRNIMSLKI
                     WGVSEVAVSDRSDHPCEEDEFLYSSSVMEEARTLVLRIFGGIPPGNRAMEEVYRVAIR
                     EAARSLTENEARPPVFFRPEFTPPSGAPLPKLAMEELVGAEVRFVSFPDVYFRISEAL
                     QSPKSSITHVAELISKDTALSATLLKLANSAIYGVPAKVDSIQRAAALIGGKALSILA
                     LGVSAVKSFNSIPSEWVNMESFWRHSVAVAVLGQIISSRKNSRIIEKVFVGGILHDIG
                     RLVLLRHVPSVMARAIAAAAEQKVPLVEMERELFGFDHGELGGSILERWGFPRELSDL
                     VRLHHAPVDETHSFPAAALSVADTLAAALRQGSSGSPHPPAISDEVWNMTDLSPGALD
                     SVVRQARRQISEIYSLFKGGDDDGKTVAHS"
     assembly_gap    1643..1742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2097..2873
                     /locus_tag="PK849_05215"
     CDS             2097..2873
                     /locus_tag="PK849_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HPE65558.1"
                     /translation="MPSFFSVLFRASAHHPLNRRLFLLAVLLFFFQSAACANELPWVE
                     HSVLRGETADSIAAKYAVSSKTVERANELAARTEPLKTGEKLLVPKKESDLLSTLAEV
                     RARRRGESPLSSITREQSKKVSLPAPRKSPLPRPAEPFIRPLAGRITSPYGMRGGRLH
                     DGVDIPAPKGTPIVASRPGKVVFSGTIQGFGRTVTIDHGDGMKTRYSHNSANLVKKGD
                     VVRQGQPIAKVGRTGRATCTHVHFSVLINGKPINPEKYLR"
     gene            complement(2975..3742)
                     /locus_tag="PK849_05220"
     CDS             complement(2975..3742)
                     /locus_tag="PK849_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65559.1"
                     /translation="MTGFRRIVCALFAVCCVLSLPAVAAEDASARFDELAARYFARME
                     GREDAVALRTALEAEAVQADEFALWRSLFREGLAPREGASNAITLLNALLDGGDPARW
                     ESASGFFLPSFVPKPLAAADAVYAAALFLLRMDGKGASDLAGMLFERLLDSPKGKYFF
                     ISAAPAEYREIVRMLTERGVVPSFGKWPEAAVEGTLPLGAPVRGRVSRDRAISEGMTF
                     LNGAGQPAPNGMYAWDRKKGRIYDVVDVERRRFWFYD"
     gene            4010..>6437
                     /locus_tag="PK849_05225"
     CDS             4010..>6437
                     /locus_tag="PK849_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPE65560.1"
                     /translation="MNIQRADSLSELVERVRSLEPFLDSLGDIVSLVDPGMRYLWASA
                     SMEAALALRRSEIVGGECFRLHHAVSSVCPHCPVSEAMSTGQVVEKELYSKERKKHFL
                     TRGIPVFGPDGRVIAAFEVTRDITDMKTRHLETEAKERYFRYLFENAPVGIFQISMDG
                     KYIAANARQALILGYESPEELMEVVNRTSVKEAHYSSSESREDVLKNLMEHPNLWTTR
                     ETEFRRRDGLLIDVQLHHRMVFSPEGEMLHIDGYMEDISERKALERKIQQQLDIQQKI
                     FDAVPLHLYLKDEKGLYAVVNQSYADFKGIPKDTFTGKTVFEIMNGHSAVIAKSEDDQ
                     VLATGEALLAEERIARGHNGIRQWHRVIKNPLRDAEGRIIGIVGCSVDITQLKEALDA
                     LRESEQRFRSLAEDMPLMISTTLADGTILYANKAYCTCFGVRRDELLGRSFLEFLVEE
                     DRKEARESIAALMPDAPISIHEERVVVADGTLRWHRWMNRAFFDSEGRAQFLQAVGED
                     ITERKLSDEAILAAKDQAQRASAAKSEFVANVSHEIRTPMNGILGLSELLLETELKDE
                     QRDFARMIHTSASNLLGVLNDILDFSKVEAGRFELNILPFSPANLVRESAELFSPQAA
                     SKELSLNLRLDGALPPHLLGDPVRLRQILINLLSNAVKFTKEGSVCVSAKLLRDDEKS
                     ADILFSVADTGPGIPDALLADIFTPFKQGEAFLNRRYGGTGLGLAISSRFVEMMGGFL
                     LVESTPGTGSSFSFSLTFEKGEERRLEDVKNQERESLPVRTRASDPTVLVVEDNAVNR
                     ELVRLLLAKAG"
BASE COUNT         1350 a         1926 c         1860 g         1201 t
ORIGIN      
        1 ccgtcacgaa acgctccgtc tggatgaccc acgcgatggt gccgtcgtcg atcagaagct
       61 gaagctcatg ctcgatacgc tccttcgcgg cgggcgagct ggcaaacgcc tgatagaaga
      121 ccgcgtcctc ctcccggaag aggtagaagc cgccctcctt cagcccgatg agcctgcccg
      181 tccgctcgta gcatacggag aggatggact ccaccgtggt ctcggcggct gcgtccggct
      241 gaatatcgtt gattttgagc gctatgtcga gcgcgttgga cagggcaagc cgttcctcct
      301 ccagcgagag gattctgttc tgaagttttt caagagtgag caactgtttt tccatcgtca
      361 tcgccgcctt tgaaaaggct gtatatctcc gaaatctgac gtcgcgcctg ccgcaccacc
      421 gaatccagcg ctccggggga gaggtccgtc atgttccaga cctcgtccga aatcgccgga
      481 gggtgcggcg agccgctgct tccctgccgc agcgcggccg cgagcgtgtc cgcgacggag
      541 agcgccgccg cgggaaagga gtgcgtctcg tcgacgggag cgtgatgcag ccggacgaga
      601 tccgagagtt cgcgggggaa cccccatcgt tcgaggatac tccctccgag ctcgccgtga
      661 tcgaatccga agagttcgcg ctccatctcc acgaggggga ctttctgttc cgccgcggcc
      721 gctatcgccc tcgccatgac ggacgggacg tgccgcagca ggacgagcct gccgatgtcg
      781 tggaggatgc ccccgacgaa aactttttct atgatacgag aattcttcct cgacgagatg
      841 atctgtccca ggaccgccac ggcgaccgag tgtctccaga aggattccat gttcacccat
      901 tcggatggga tgctgttgaa ggatttcacc gccgatactc cgagggcgag gatggagagc
      961 gccttgcccc cgatgagggc ggcggcgcgc tgaatggagt ccaccttcgc cggaacgccg
     1021 tagatggcgc tgttggcgag tttcagaagc gtggcggaga gggccgtatc cttgctgatg
     1081 agttcggcca cgtgcgtgat ggagcttttg ggggattgaa gcgcctcgga tatgcggaag
     1141 tagacgtcgg ggaaggagac gaaacgaact tcggccccga caagctcttc catcgcgagc
     1201 ttcggcagcg gcgctccgga aggcggggta aattccggcc tgaaaaaaac ggggggacgc
     1261 gcttcattct ccgtcaggct tcgcgcggcc tcgcggatgg cgacccgata gacttcttcc
     1321 atcgcccggt ttccgggggg gatgccgccg aatatgcgga ggacgagcgt gcgcgcttcc
     1381 tccatgacgg aggaggagta cagaaattcg tcctcctcgc agggatgatc cgaacggtcc
     1441 gacacggcaa cctcggatac tccccagatc ttcagactca tgatgttcct ggacgtaagg
     1501 catgcaccct tcggcagaat gaaccgcccg ttcggggcga ccaagtccgc cgaaagaatc
     1561 attccggatt tcagttgagt gatggaaagt gttcccatgc aaccccccgg aattcccctc
     1621 acggttcaaa ggggagaata aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnagtgttcc catgcaaccc cccggaattc ccctcacggt tcaaagggga gaataaaaaa
     1801 aattttacac cgctctcctt tttaaccaca gagtgcacag cctgtcaagg gctcggagag
     1861 aggttttgtg aacgaactgt gatattgtca tcccaaaaga gggaggggga aaaatatcgt
     1921 cgctcgttgc cggaaaaagc ctccgaacca ggagaacgga agaaatgtca tctgttcttc
     1981 tcaaggaagg ggcctccgtt gtggatccgt tctcagtaat tttactgtcc gcaacctttt
     2041 gttttcatgt atcataccgg agatacacat aaaaaaaagt tcaggaggaa ctccgcatgc
     2101 cctctttctt cagcgtcctg ttcagggctt ctgcgcatca tccgttgaac cgtcgtcttt
     2161 ttcttctcgc tgttcttctt ttcttcttcc agtccgccgc gtgcgcgaac gaacttccgt
     2221 gggtcgaaca ctcggtcctc cgcggagaga ccgccgactc catcgcggcg aagtacgccg
     2281 tttcctcaaa gaccgtcgaa agggccaatg aacttgccgc ccggacggaa cctctgaaga
     2341 cgggggaaaa gctcctcgtt ccgaagaaag aatccgatct cctctccacc ctcgccgagg
     2401 tccgcgcccg gcggcggggc gaatcaccgc tcagttcgat cacgagggag cagtcgaaaa
     2461 aggtttctct tcccgcgccg aggaagagcc ccctgccgcg accggccgag cccttcatcc
     2521 gtccgctcgc cggaagaatc acctctccct acggaatgcg gggcggacgc ctgcacgacg
     2581 gcgtggatat cccggctccc aagggaacgc cgatcgtcgc gtcgcgcccc ggcaaggtcg
     2641 tcttctccgg aacaatccag gggttcggcc ggacggtaac catcgaccac ggcgacggga
     2701 tgaagacccg gtacagccac aacagcgcca acctcgtcaa aaaaggggac gtcgtccgtc
     2761 aggggcagcc tatcgcgaag gtgggcagaa ccggaagggc gacctgcacg cacgttcact
     2821 tcagcgtcct tataaatggt aagccgatca acccggagaa gtaccttcgc tgagcggaaa
     2881 cttcctttat cttcaaagaa aagaaggcgc gtcccgaaaa cagggaacgc gccttcttcc
     2941 ttataaatgg aactacaaga aagctgcata aggatcagtc gtagaaccag aagcggcgcc
     3001 gctccacgtc gacgacgtcg taaattctcc ccttcttcct gtcccacgca tacattccgt
     3061 tcggcgcagg ctgtccggcg ccgtttaaaa aggtcattcc ctccgagatc gccctgtcgc
     3121 gggatacgcg cccgcgaacc ggggccccca acggcagcgt cccctccacg gcggcctccg
     3181 gccactttcc gaaggaggga acgacgcctc gctccgtcag catccggacg atttcgcgat
     3241 actccgccgg cgcggcggaa atgaagaagt actttccttt cggggagtcc agcaggcgct
     3301 cgaagagcat accggcgaga tccgacgcgc ctttcccgtc catccgcagc agaaagagcg
     3361 cggcggcgta gacggcgtcg gcggcggcca gcggcttcgg gacgaaggag gggaggaaga
     3421 acccggaggc gctctcccac cgcgcaggat cccctccgtc gagaagcgcg ttcagaaggg
     3481 tgatcgcgtt cgaagcgccc tccctcggag caagcccttc gcggaagagg gagcgccaca
     3541 gagcaaactc gtcggcctgc acggcctccg cttccagggc ggtccgaagc gcaacagcat
     3601 cctcacgccc ttccattctg gcgaagtagc gcgccgccag ctcgtcgaaa cgcgccgacg
     3661 catcctccgc ggcgaccgca ggcaaggaga gaacgcagca cactgcaaaa agagcgcaga
     3721 ctatgcgccg aaaacccgtc atgagcatca actccttcag ctcctttaga gacatcgaat
     3781 tatcttaggt gcttcatcca ttctaccact caaaattagt cagtaatgat caatcattat
     3841 atactcttct ttcgcgtctg aaaacccggc ggagcgcaaa aaacccgccg tgaagcggaa
     3901 aactccccga tggtttcact tttcgcgcgc aatgatcaca tccctctctc tttcccgtgc
     3961 tctatcctgt agaatatacg tatgatgcgg gaaagggagc gatagaggaa tgaacattca
     4021 acgggcggac tctctcagcg aactggtcga acgggtacgt tccctggagc cgtttctcga
     4081 ctccctcggg gatatcgtct ctcttgtcga tccagggatg cgctacctct gggcatccgc
     4141 ttcgatggaa gccgctctcg ctctccgccg ttcggagatc gtgggggggg agtgtttccg
     4201 ccttcatcac gccgtttcct ccgtctgccc gcactgtccc gtcagcgagg ccatgtcgac
     4261 ggggcaggtc gtcgaaaaag agctgtactc caaagaacga aagaaacact ttttaaccag
     4321 ggggattccc gtcttcggtc ctgacggtcg cgtgattgcg gcctttgaag tcacccgcga
     4381 tatcaccgat atgaagactc gtcaccttga gacggaggcg aaggagcgct acttccgcta
     4441 cctcttcgag aacgcacccg tcgggatctt ccaaatttcc atggacggta aatatatcgc
     4501 ggcgaacgcg cgccaggcgc tcatccttgg gtatgagtct ccggaggagc tcatggaggt
     4561 cgtcaacaga acatccgtca aggaagcgca ttactcttcc tccgagtcca gggaagacgt
     4621 tctgaaaaat ctcatggagc atccgaacct gtggaccacc cgggagaccg agtttcgcag
     4681 acgcgacggc ttgttgatcg acgtccaact tcaccacagg atggtctttt ctccggaggg
     4741 ggagatgctc cacatcgacg gctacatgga agacatctcg gaaagaaaag ccctggagcg
     4801 aaagatccag cagcagctgg acatccagca gaagatcttc gacgcggtgc cgctgcacct
     4861 ctatctgaag gacgagaagg ggttgtacgc ggttgtgaat cagtcgtacg ccgacttcaa
     4921 gggaattccg aaggatacgt tcaccgggaa gacggtgttc gagatcatga acggacacag
     4981 cgcggtcatc gcaaagtccg aggacgatca ggtgctggcc accggagaag cgcttctggc
     5041 ggaggagcgg atcgccaggg gacacaacgg catcaggcag tggcacaggg tcatcaagaa
     5101 ccctctccgc gacgccgagg ggaggatcat cggcatcgtc gggtgcagcg tcgacatcac
     5161 acagctcaaa gaggccctcg acgcgctccg ggagagcgaa cagcgcttcc gttcgctggc
     5221 ggaggatatg cccctgatga tctccaccac gcttgccgac ggaacaatcc tctacgcgaa
     5281 caaggcgtac tgcacctgct tcggcgtcag aagggacgaa cttctcgggc gctctttcct
     5341 ggaatttctt gttgaggagg acagaaagga ggcgcgcgaa tccatcgccg cgctgatgcc
     5401 agacgccccc atctccatcc atgaagagcg cgttgtcgtc gccgacggca cgctccgctg
     5461 gcaccgctgg atgaaccgcg cgttcttcga ctcggagggg agggcgcagt ttcttcaggc
     5521 cgtcggagag gatatcacgg agcgaaagct ctccgatgag gcgatcctcg ccgcaaaaga
     5581 ccaggcgcaa cgggcgagcg cggcgaaatc cgagttcgtc gccaacgtga gccacgagat
     5641 ccgcacgcct atgaacggga ttctcggcct ctcggagctt ctgctggaga cggagctgaa
     5701 ggacgagcag cgggacttcg cgcggatgat ccacacaagc gcctccaatc tgctcggcgt
     5761 gctcaacgac atcctcgact tttcgaaggt cgaagcgggg cggttcgagc tgaacatcct
     5821 cccgttttcc ccggccaacc tcgtgaggga gtcggcggag ctcttctccc cccaggccgc
     5881 ttccaaggag ctctcgctca atctgcggct ggacggcgcc ctccccccac atctgctcgg
     5941 cgatccggtc cgcctgcgcc agattctcat caatctgctc tcgaacgcgg tgaagttcac
     6001 gaaagaggga agcgtctgcg tctccgcgaa gctcctccgc gacgatgaaa agagtgccga
     6061 tattctcttc tccgtcgcgg acacgggtcc cggaattccg gacgcgcttc tggcggatat
     6121 cttcaccccg ttcaagcagg gggaggcctt cctgaacaga cgatacggcg gcaccgggct
     6181 cggactggct atttcaagcc gtttcgtcga gatgatgggg gggtttcttc tggtcgagag
     6241 cacgccggga acgggctcct cattctcttt ctccctcacc ttcgagaagg gagaggaacg
     6301 acggctggaa gatgtgaaaa accaggagag ggaatccctt ccggtgcgaa cccgcgcctc
     6361 cgacccgacg gtgctggtgg tggaggacaa cgcggtaaac cgcgaacttg tccggctgct
     6421 gctggcaaag gccgggt
//
LOCUS       DAOPKU010000122         6389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_12982_length_6389_cov_4.269814, whole genome shotgun sequence.
ACCESSION   DAOPKU010000122 DAOPKU010000000
VERSION     DAOPKU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12982_length_6389_cov_4.269814"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PK849_05230"
     CDS             complement(<1..851)
                     /locus_tag="PK849_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE65561.1"
                     /translation="MKISEFFVQNFGIFSGSGTSLSKGLTIFHGENESGKTTLMNFFR
                     RILFPREKYSRTRGNIYEPLSGARHGGSARVRMEDGREFVLTLDGKKNFVLPASGGSA
                     APLPSNFFSIGKDVYESVFAMGLNDMQSMESLNKADIAARFFSAGSGLGSASLPRLLS
                     SLEERGNELYRPGLAKGASAVNRLLASMKETDERIRELRMASGSWGERRDALDSAERA
                     LEQRREELAELQRRLSFLELLEKGLAPRAALGEIERSLGELSALPPFPEGGLDKLERL
                     KDERKRL"
     gene            complement(848..2158)
                     /locus_tag="PK849_05235"
     CDS             complement(848..2158)
                     /locus_tag="PK849_05235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HPE65562.1"
                     /translation="MGSGTLKFIHCADLHLDSPFSGLAETSPALGSFLRAASFSAFRN
                     AVDCALRNEADFVLISGDVYDGEDRSVRAQLFFLEQLKRLSDAGISSFTVHGNHDPLS
                     GWELDRDLPPLAYRFGSSEVESVPLTVKGERVGTVHGCSYPVRDVRENLARGFAGRRT
                     EGVNIALLHSNVGGRKGHENYAPCSLDDLRAAGMDYWALGHVHSAEILCHDPLVVYPG
                     NIQGRHIGETGKKGVFSVSMGSAGGHPGGRGGVEFIPCDVVRWRREALSIEGMQRDEE
                     LFAAFDRLRDGVRNEAGGRPVIVRASVGGRGKLSALLRRPGFLSGPGGLVESLNQGEE
                     GRSDFVYIESVVDDTASPFDLEALSSGNHFVGDFLKEVSAFERRGNLREGLMEILSAR
                     GVREKLPREVFDRVDALPDDEILSLLRRGTTLALAGLLEGEDDE"
     gene            2426..3439
                     /locus_tag="PK849_05240"
     CDS             2426..3439
                     /locus_tag="PK849_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPE65563.1"
                     /translation="MKGFGAGVRAISYYLPPKVVDNKALVEEFGTWTEEKIYQKTGIR
                     ERHIVDGELVSDLATRAGEKFFEEHGIGRDEIDFLLLCTESPDYYLPATACVVQDRLG
                     LKKTTGALDFNLGCSGFVYGLALAKGLIGTGIAKKVLLITADTLSRTVHPMDKSTRTI
                     FGDAAAATLIEGSDTARIGDFVLGTDGSGMDKLIIPAGAWAAPRSPETAVERTNKWGN
                     TRSAENMYMNGPEVLKFTVETVPPAVQQALDVHSLKLEDIDLFVFHQATKLILEHLRK
                     EIGIPEEKFYTNIENKGNTVSATIPMALRDAADEGRLRPGDRVMVVGFGVGFSWGATI
                     LRW"
     gene            3532..4680
                     /locus_tag="PK849_05245"
     CDS             3532..4680
                     /locus_tag="PK849_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPE65564.1"
                     /translation="MRGFALRKFVAPEILFGRGALSLAGQYARNLGGGRVFVATDPGV
                     IEAGLLEPVLESLEQEDLDYTVFSDIRPNPTASMVMAGARVFADERCDLILALGGGSS
                     MDCAKAVGAVHANASHVSEFVGVDRIPLPCPPVICIPTTAGSAADVSQFAIIKNEETK
                     VKNAIVSKSIVPDVALVDPETTLTMDRNLTAATGLDALTHAVEAYVSRASSDLTDLHA
                     LQAIRLVGEFLPKVLDNLSDIDYRTGMMLASLHAGLAFSNASLGVVHALSHSLGGLLD
                     LPHGECNSLLLRWGVEANFSAAPERYTQILEALGGNTSSPEPMKELLDRLDALRSTAG
                     IPGRLRDLGLSEEQLPVLAKTGEEDPCMLTNPLPFNHDELEELLRNAY"
     gene            4670..5749
                     /locus_tag="PK849_05250"
     CDS             4670..5749
                     /locus_tag="PK849_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPE65565.1"
                     /translation="MPIDGNDEQFLALMGLGAKSGKKSHYPELKRRLSELERMRDLLD
                     QARDAIFLLALPEEIVEYQNEAALGLLGLVRTPEKRLLQECLKTSQGASLSLFSLFPS
                     LSSEEQRKVVPLLTRNGTRRFEASFQAAVESGRTSGILILRDQEERIAIQEQLAESLK
                     AVEEARMKTVRLTAAMVEMKDSYTGKNQRQAARLAHEIGHRLELPKPEIDDLVTSALL
                     HDVGMMGVPTEILCIPGPLRPVDRRLMQEHVTIGRDILVKEGFPERIALAVLHHHERM
                     DGSGYPDGLKGEEIPLFARVVGIADVAEAMLSHRPYRPAHSREEVLRELEEHQGTLYD
                     RRTAEICISLLLEGFSVSEETGNKY"
     gene            complement(5805..6113)
                     /locus_tag="PK849_05255"
     CDS             complement(5805..6113)
                     /locus_tag="PK849_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPE65566.1"
                     /translation="MKIDVSRSGNGARISISGSMYVEDSASVREKLIALLDEGVYNLT
                     LDLSGLDYVDSSGLGVLISIHKRCLQKGGKMTITGLRGMVEELFKLTRLDLVFNVAAH
                     "
     gene            complement(6202..>6389)
                     /locus_tag="PK849_05260"
     CDS             complement(6202..>6389)
                     /locus_tag="PK849_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65567.1"
                     /translation="ETTDDHVQNLAASMEETAAAMENVATGSQEVSETAEDLKEMTGR
                     FRVEKDEEGWTNVPAVV"
BASE COUNT         1220 a         2058 c         1944 g         1167 t
ORIGIN      
        1 tcaagccgct tccgttcgtc tttcaagcgc tccagcttgt caagccctcc ctcagggaac
       61 ggaggaaggg cggagagttc gccgagggat cgctcgatct cgccgagcgc ggcgcggggc
      121 gccagcccct tctccagaag ctccagaaag gagagccgcc gttgcagttc cgcaagttcc
      181 tcgcgccgct gttccagcgc gcgctccgcg ctgtcaagcg catcgcggcg ctcaccccac
      241 gaaccgctcg ccatgcgaag ctcgcggatc cgctcgtccg tctccttcat cgacgccagc
      301 aggcggttca cggccgaggc gcccttggcg agtccgggcc ggtagagctc gttcccccgc
      361 tcctccaatg aggagagcag cctgggaagc gatgccgaac ccaggcccga accggcggag
      421 aagaagcgcg ccgcgatgtc ggccttgttc agcgattcca tcgactgcat gtcgttcagc
      481 cccatcgcga agacgctctc gtagacgtcc ttgccgatgg agaagaagtt cgacggaagc
      541 ggcgcggcgc ttcctcccga cgccggaagg acgaagttct tcttcccgtc gagcgtgagg
      601 acaaactccc tgccgtcctc catgcggacc ctggcggaac cgccgtgccg cgcgccgctc
      661 agcggctcgt agatgtttcc tcgggtgcgc gagtacttct ccctcgggaa aagaatccgc
      721 cggaagaagt tcatcagcgt cgtctttccg ctctcgttct cgccgtgaaa gatcgtcagc
      781 cccttggaga gagaggttcc cgacccggaa aaaataccga agttctgtac gaaaaactcg
      841 ctgatcttca ttcgtcgtcc tccccctcaa gcagtcccgc aagcgcaagc gtcgtccctc
      901 tgcgcagcag cgagaggatc tcgtcgtcgg ggagcgcatc caccctgtcg aacacctcgc
      961 gggggagctt ctccctgacg cccctcgcgg agagtatctc catcaaccct tccctgagat
     1021 tgcctcggcg ctcgaaggcc gaaacttcct tgaggaagtc gccgacgaaa tggttccccg
     1081 aggacagcgc ttcgaggtcg aacggggacg cggtgtcgtc gacgacgctt tcgatgtaga
     1141 caaagtcgct ccgcccctcc tctccctgat tcaggctctc cacgagcccg ccgggaccgg
     1201 agaggaaccc ggggcgtcgc agaagcgcgc tcagcttgcc gcggccgccg accgacgccc
     1261 ggacgatcac agggcgtccg cccgcctcgt tccgaactcc gtccctgagg cggtcgaagg
     1321 cggcgaagag ctcctcgtcg cgctgcattc cttcgatgga gagggcttcc ctccgccagc
     1381 ggacgacgtc gcacgggatg aactccacgc cgcctctccc gccgggatgc ccgcccgcgg
     1441 agcccatact taccgagaag acgcctttct tgcccgtctc gccgatatgc cgcccctgaa
     1501 tgttgccggg gtagacgacc agcggatcgt ggcagagaat ttccgcggag tgcacgtgcc
     1561 ccagcgccca gtagtccatc cccgccgcgc ggagatcgtc cagcgagcag ggggcgtagt
     1621 tttcgtgccc cttccttcct ccgacgttgc tgtgaagcag ggcgatgttc acaccctccg
     1681 ttcgccgtcc cgcgaagccg cgcgccaggt tctccctgac gtcgcgcacg gggtagctgc
     1741 acccgtgcac cgttcccacc cgctcgccct tcaccgtcag cggcacggac tcgacctccg
     1801 acgagccgaa ccggtacgcc aggggaggca ggtcgcgatc cagctcccac ccggagagcg
     1861 gatcgtggtt gccgtgcact gtgaacgacg agatccccgc gtcggaaagc cgtttcagct
     1921 gttcgaggaa gaagagttgc gcgcggacgc ttctgtcctc gccgtcgtac acatctccgg
     1981 agatcaggac aaagtccgcc tcgttccgca gcgcgcagtc gacggcgttc ctgaaggcgg
     2041 agaaggaggc cgcgcgcaaa aagctcccca gcgcggggga ggtctccgca agcccggaga
     2101 aggggctgtc gagatgaaga tccgcacaat gtatgaattt cagcgtgccg ctgcccatga
     2161 gagcgccact cctttcattc ggagcaccgg ggaacgcgtc ggagcgtctt cccggagccg
     2221 gtcttcttcg cggagcggtt ttccgggccg cgaaaggtgt ccttacttta ttccacccgc
     2281 cgtttctttg caagagcatt cagcgcgcat tttcaggaca acggaagact tgtcacttat
     2341 cggtgacaat gggataatag catcgcgtcg agtatgatgc acattctgta aataaggaac
     2401 atccagttat ggaggcgttc gagagatgaa aggatttgga gccggagtac gggctatttc
     2461 atactacctt ccgccgaagg tggtggacaa caaggcgctg gtggaggagt tcggcacatg
     2521 gaccgaggag aagatctacc agaagacggg tattcgggag cgccatattg tggacgggga
     2581 gctcgtctcc gatcttgcga cgcgcgccgg tgagaagttt ttcgaggagc acggcatcgg
     2641 aagagacgaa atagactttc tgcttctgtg caccgagagc cccgactact acctcccggc
     2701 aaccgcttgc gtggtgcagg acagactggg gctgaaaaag acgacggggg cgctcgactt
     2761 caacctgggc tgctcggggt tcgtctacgg gctcgccctc gcgaagggac tgatagggac
     2821 ggggatcgcg aagaaggtgc tgctgatcac ggcggacacg ctgtctcgca ccgtccatcc
     2881 gatggacaag agcacgcgca cgatcttcgg cgacgccgcc gcggcaacgc ttatcgaagg
     2941 ttcggacact gcgcgcatag gggattttgt gctgggaacg gacggctcgg ggatggacaa
     3001 gctgatcatt ccggccgggg cgtgggcggc cccgcgatcg ccggagacgg ccgttgaacg
     3061 gacgaacaag tggggcaata cgcggagcgc ggagaacatg tacatgaacg gccccgaggt
     3121 gctgaagttc accgtggaga ccgttccgcc cgcagtacag caggctctgg acgtgcactc
     3181 gctgaagctg gaggacatcg acctcttcgt tttccatcag gcgacgaagc tgattctgga
     3241 gcacctccgc aaggagatcg gcattccgga ggagaagttc tacaccaaca tcgagaacaa
     3301 ggggaacacc gtgagcgcga ccattccgat ggccctgcgc gacgcggccg acgagggaag
     3361 attgcgcccc ggcgacagag tcatggtcgt cggcttcggc gtcggcttct cctggggagc
     3421 gacgattctc cgctggtaag ggagagtacc gcaacattat tccatttttg tgataaaata
     3481 aaggcggggg aatacacccc cgcctttatt gtgacaaaag gggatgagag tatgcgcggt
     3541 tttgccctga gaaagttcgt cgcccctgaa atactcttcg ggagaggggc cctctccctc
     3601 gcgggtcagt acgcccgtaa tctcggcggc ggcagggttt tcgtcgccac ggatcccgga
     3661 gtcatcgaag cgggcttgct tgaacccgtc cttgagagtc tcgaacagga ggaccttgac
     3721 tacactgtgt tctccgatat tcggccgaat cccactgcct cgatggtcat ggcgggggcg
     3781 cgcgtcttcg cggatgagcg gtgcgacctg atcctggccc tgggaggcgg aagctccatg
     3841 gactgtgcaa aagcggtggg cgcggtccac gcgaacgcct cccacgtgag cgagttcgtc
     3901 ggcgtcgacc ggataccgct cccctgtcct ccggtgatct gcatcccgac gaccgccgga
     3961 agcgcggccg acgtctccca gttcgccatc atcaagaacg aagagacgaa ggtgaagaac
     4021 gccatcgtca gcaaatccat cgtcccggac gttgcgctcg tcgaccccga aaccacgctc
     4081 acaatggacc ggaacctcac cgccgccacc ggactggacg cgctgacgca cgcagtcgag
     4141 gcctacgtct cgcgggcgtc gtccgatctt accgacctgc acgcccttca ggccattcgc
     4201 ctcgtgggcg agttccttcc caaagttctg gacaacctct ccgacatcga ctaccggacc
     4261 ggcatgatgc tcgccagcct gcacgccggg ctcgccttct ccaacgccag cctcggcgtc
     4321 gtccacgcgc tctcccacag cctcggcggc cttcttgacc tcccgcacgg cgagtgcaac
     4381 tcgctgctgc tccgatgggg cgtggaagcg aacttttcgg cggcaccgga gcggtatacg
     4441 cagattttgg aagccctcgg gggaaacacc tcctctccgg aaccgatgaa ggagctcctt
     4501 gaccggctgg acgcgctccg ctcaacggcg ggaatccccg gcaggctccg tgatctgggg
     4561 ctcagcgagg aacagctccc cgttctggcg aagaccggcg aggaggaccc gtgcatgctg
     4621 acgaacccgc tccccttcaa ccatgacgaa ctggaggagc tgcttcgcaa tgcctattga
     4681 cggcaacgac gaacagtttc tcgcccttat ggggcttggc gccaaatcgg gcaaaaagag
     4741 ccactacccc gagctgaagc gccgcctttc cgaactggag cgaatgcgcg acctgctgga
     4801 tcaggcacgg gacgcgatct ttcttcttgc gcttccggag gagatcgtgg agtaccagaa
     4861 cgaagcggcc ctcgggctcc tcgggcttgt ccgcaccccg gagaagcggc ttttgcagga
     4921 gtgtctcaaa acgtcgcagg gcgcgtccct gtcgctcttc tcgctctttc cctctctctc
     4981 ctccgaggag cagcggaaag tagttcctct gctcacccgg aacggaacgc gccgcttcga
     5041 agcgtccttt caggccgccg tggagagcgg acgaacatcg ggtattctca tcctccgcga
     5101 ccaggaagag cgcatcgcca tccaggagca gctcgccgag tcgctgaagg cggtcgagga
     5161 ggcgcggatg aagacggtcc gcctaaccgc cgcgatggtg gagatgaagg actcgtacac
     5221 ggggaagaac cagcgccagg cagcccggct ggcgcacgaa atcggccata gactcgaact
     5281 gccgaagccc gagatcgacg acctcgtcac ctcggcgctt cttcatgatg tggggatgat
     5341 gggcgttccc accgagatcc tctgtatccc cgggccgctt cgccccgtcg acaggcggct
     5401 gatgcaggag cacgtaacca tcgggagaga tatcctggtg aaggagggct tccccgagcg
     5461 gatagcgctc gccgtgctcc atcaccacga acggatggat ggcagcgggt acccggacgg
     5521 cctgaagggt gaggaaatcc cgctcttcgc gcgcgtcgtg ggcatagcgg acgtggcgga
     5581 agcgatgctg agccatcgtc cgtaccggcc ggcacactcg cgggaagagg tgcttcgcga
     5641 gctggaagag catcagggaa cgctctacga cagacgaacg gcggaaatct gcatctctct
     5701 gcttctggaa gggttctccg tttcggagga gaccggcaac aaatactgaa cggcaaaggg
     5761 gccccctcgg gagccccttc tttttacgcg cagtctttga gaactcagtg cgcagccacg
     5821 ttgaagacga ggtcgagccg agtgagtttg aagagctcct cgaccatgcc gcgaaggccg
     5881 gtgatcgtca tcttgccgcc cttctgaagg caccgcttgt ggatggagat gagcacgccg
     5941 aggcctgagc tgtcgacgta atccaacccc gagagatcca gcgtcaggtt gtacacgcct
     6001 tcgtcgagaa gcgctatcag cttctcccgc accgatgcgg aatcctccac gtacatactt
     6061 ccggaaatgc tgatccgggc tccgttcccg ctcctgctga catcgatttt catggacgct
     6121 cctccttata tatatcgaga catcttgtta cactccaaca ctgcataaac atacgttctt
     6181 cccacgaaga tgcaggctcc attatacgac agccggcacg ttcgtccacc cctcctcgtc
     6241 cttttcgacc ctgaaccgcc ccgtcatttc cttcaaatcc tcggcggtct cgctgacctc
     6301 ctgcgatccc gtggccacat tctccatggc cgcggcggtc tcctccatcg acgcggcgag
     6361 gttctgcacg tggtcgtccg tggtctcca
//
LOCUS       DAOPKU010000123         6372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_13033_length_6372_cov_4.500079, whole genome shotgun sequence.
ACCESSION   DAOPKU010000123 DAOPKU010000000
VERSION     DAOPKU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13033_length_6372_cov_4.500079"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PK849_05265"
     CDS             <1..724
                     /locus_tag="PK849_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPE65568.1"
                     /translation="ALGIDGRKLLAGAAEMDEKLAQRASLFENPAWLLSALSVAHYRR
                     GRNMTVMMPYEDALERFCEWFAQLWGESLGKNGVGSTPVRALGAIDQHSQIQLYTEGP
                     DDKLFSIITVSEPEEDIVIPETEEQALVPLGYLFGQSMNSLRRYEALSTAAAVAKGGK
                     PVVTIEVPRLDERRLGALIQLYEHVTALTGYVLDVNPFDQPGVEQGKNYTYGLMGRPN
                     FSAQALEVTELAEKLSLMEVTL"
     gene            complement(791..2629)
                     /locus_tag="PK849_05270"
     CDS             complement(791..2629)
                     /locus_tag="PK849_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPE65569.1"
                     /translation="MGKNEEYSVQLPPSGQFSPSEKSASFTENEEIVRRYFRWEPLDD
                     PSFAHLIVGKGSIGGKGRSLLFAMAKLRDSGVETLKRVVFPPSLFIATDSFDSVVSQI
                     PNLEALKKGSPETIEEAFLATKLPDSVLGAIRKFLEKITDPIVVRSSSVLEDSLKYSF
                     AGKYLSTFEFNGGDESLEERAAHVESDIKKIYARTFFPVAVAYRQKHGLGDDRMGIIL
                     MRVAGKWRGRYYYPTMAGVGFSRYYRRWTTRIKSEDGIIRLVFGMGTTSTKRGYARTF
                     ALTLPSLRPEGQNPFNIMRHSQEHFQVIDREQKALVTVDVKQIWKDIFPYHSCFPDYA
                     QIYSPDEEGGYFTPMSRHGSVIEKGEKVCFTFEEFAFRSKHFFERMKQLLSLLDREMG
                     VPADVEFAFHPTEDLVEIVQARPLWISEQTSTATIPPLENRNVVLRADRMVTHGVKEN
                     IPYLVYIDHHVYAQETRFHDVARAVGSVNDLLKGSRFVLVAPGRVGSSNPLLGVPVQY
                     NEITRCCCIVEVGFPKEGYMPELSFGTHFFTDLEIDGILYMPVYEGAKNNIFDEHFFD
                     SSPYTLGGHAGVRIYSGSFSVYTDGDKNLGVVVADRVDEPESGWAE"
     gene            complement(2719..3519)
                     /locus_tag="PK849_05275"
     CDS             complement(2719..3519)
                     /locus_tag="PK849_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65570.1"
                     /translation="MTEKNTSTLPFSIRKKMGLAITEFQMITSGDRIMIGLSGGKDSL
                     MLATALSGLRRRSPVKFSLSACTVDITGGETNTESLRAFCESLDISYSVKTHPIVNII
                     NIRNERSPCSLCANIRRGILNAAVNESGCNVLALGHNLDDAVETALMNLFHTGRFKSF
                     QPKFWQSRSGVTVIRPLILVEERRILKEVSRLELPVLPYICPFSLETERARAKKIIDQ
                     LAAGNPRIKYNIIHALKSIDEGDRWELSAHRKKQEHSPGSGSFVPILD"
     gene            3665..4351
                     /locus_tag="PK849_05280"
     CDS             3665..4351
                     /locus_tag="PK849_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE65571.1"
                     /translation="MEQIQVLIADDHKLFRDGVKKLLELESDIVVIGEAGDGEEALLL
                     IREHEPDILLFDINMPRVDGIQLVRELNNIPHKMRYVAISAYDDEDCLSALSAAGVLG
                     FVLKASGKVELLSAIRSAARGQPYVDPRVAGKLLTSFSRRKEENDQLYELTPREKEIL
                     YWLSQGLSNTEVADRMVLSEKTVKNHVSHVLKKLDLRDRTQAAILAWRIGFAQLSPDT
                     LAHILESEVR"
     gene            complement(4462..>6372)
                     /gene="fliD"
                     /locus_tag="PK849_05285"
     CDS             complement(4462..>6372)
                     /gene="fliD"
                     /locus_tag="PK849_05285"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HPE65572.1"
                     /translation="QDTLELKIGLFDEFSASLKSLRSVVSPLKLESVFKAKAAEFTAL
                     GGVDASSIVTATVDASAAIARHEIDVTQKAVAETRFSTQISTTMEEAGLDQTRTFQIN
                     VGGRRADIEVLKTDSLQDIADKINTAIDATIDPSTGEAYGEGLGITATVIDNRLILKS
                     SNTGLGTTKTDWDVTRGAGTTDKLGFTVAKGAPSNGTLVISDGTNTYTEGTDFTVTSG
                     SDVIDWSMGGIEPAAGVTYTVSYTVNSNAFSLTGEPEVLTLLDLDDSTIGDPDYNLQA
                     QDAIFKIDGLSITRSSNQVDDLLEGVKLTINGPGTVIMDITQDAEQAVTGVQDFVTAY
                     NDALDWINIRLSESTQEDADDDFSKKFGLLHGNSMLWQSKSQLRMLMTSSVTAKYSTK
                     TGSTVLGTMASRGLSSNSTFELTVGVRTAQIEVTPSDTLSTIASKINTSYEMLHDEQG
                     RTYPIPMASAKVVNNQLVIEASPGRKFSLSASDDVLDTLGLGTPFTLLSQLGISTESA
                     DYGKSGKLEFDTDKFMEALREDPEGVAAIMNTMMTSMDDYIGNMVDTSQVQVGDTTAP
                     KGRIASQIYTFRSEITTLDKRISDLERRLEVRARGLYESFANAEVHLAKLQQQASWLS
                     STLSQLTGGSGN"
BASE COUNT         1384 a         1703 c         1750 g         1535 t
ORIGIN      
        1 cgctctcggg atcgacggca gaaaactcct tgcgggagcg gcagagatgg acgagaagct
       61 cgcgcagcgc gcttctcttt tcgagaatcc ggcatggctt ctttccgccc tctcagtggc
      121 gcactaccgg cgtggacgca acatgacggt catgatgccg tacgaagacg cgctcgaacg
      181 cttttgcgag tggttcgccc agctctgggg cgagagcctg ggaaagaacg gcgtcgggtc
      241 gacccccgtc cgcgctctgg gcgccattga tcagcactcg cagattcagc tgtacactga
      301 agggcccgac gacaagctct tttccatcat cactgtgtcc gaaccggagg aggatatcgt
      361 gatccccgag accgaggagc aagcgctcgt cccgctcgga tacctttttg gccagtcgat
      421 gaattcgctg cgccggtacg aagcgctttc gactgccgct gctgtcgcca aaggagggaa
      481 accggtcgtg accatagagg tcccgcgctt ggatgaacgg cggctcggcg ccctgattca
      541 actctacgaa catgttacag cgctcaccgg atatgttctt gatgtgaacc cgttcgacca
      601 gcccggggtg gagcagggga agaattacac ctacggactg atggggcggc ctaatttcag
      661 cgcccaagct cttgaagtga cggagcttgc cgagaaactt tctcttatgg aagtcaccct
      721 ctgagttttt ctgaaaaaaa agaaggccgc gcaggagcaa ctctccgcgc ggccttcgca
      781 gtatcgaaaa ctattccgcc caccctgact cgggttcgtc caccctgtcc gccacaacga
      841 cgcccaaatt tttgtctccg tcggtgtaca ccgaaaagga gcccgagtag atacgcactc
      901 ctgcgtgtcc gccaagagta tagggagagg aatcgaagaa gtgttcgtcg aagatgttgt
      961 tctttgcgcc ctcgtacacc ggcatgtaca gaataccgtc tatctccagg tcggtgaaga
     1021 agtgcgtgcc gaacgagagt tcgggcatgt acccctcctt tggaaaccct acctctacga
     1081 tgcagcagca gcgggtaatc tcgttgtact ggacgggaac tcccagcagc gggttgctcg
     1141 aaccgacgcg tcccggcgcc acaaggacga atctcgagcc tttcaacaaa tcgttcacag
     1201 aaccaaccgc cctcgcgacg tcgtgaaatc gcgtctcctg cgcgtagacg tgatggtcga
     1261 tatagacaag ataagggatg ttttccttta cgccgtgcgt aaccattcga tccgcgcgca
     1321 gcacgacatt gcggttttca agaggaggta tggtcgccgt agacgtctgt tcgctgatcc
     1381 agagggggcg ggcctggacg atctccacaa gatcctcggt cgggtggaac gcgaactcga
     1441 cgtcggcagg aacacccatc tccctgtcaa gaagagagag caactgtttc atcctctcga
     1501 aaaagtgttt tgaacggaat gcgaattctt caaaagtgaa acagaccttt tctccctttt
     1561 cgatgaccga gccgtgacgg gacatcggtg tgaagtagcc tccctcctca tccgggctgt
     1621 agatctgcgc gtagtcgggg aaacagctgt ggtaagggaa gatatccttc catatctgtt
     1681 tgacgtccac tgtgacaagc gccttctgtt ctctgtctat cacctggaag tgttcctggg
     1741 agtgccgcat gatgttgaaa ggattctgac cctccggacg gagcgaaggg agcgtcagtg
     1801 cgaacgtgcg ggcgtaccct cgtttcgtgc tggtggttcc catgccgaag acgaggcgga
     1861 tgatgccgtc ttcggattta atcctcgtgg tccatcgtcg gtagtaccgg gagaaaccaa
     1921 cgcccgccat ggtcgggtag tagtagcggc cgcgccactt gccggcgacg cgcatcagga
     1981 taattcccat tctgtcgtcg cccagtccgt gcttttggcg ataggcgacc gcaaccggga
     2041 aaaacgtgcg cgcgtaaatt tttttgatgt cgctttctac atgagcggct cgttcttcga
     2101 gagattcgtc ccctccgttg aattcgaacg tcgagaggta tttcccggcg aaggagtatt
     2161 tgagagaatc ctccaggacg ctgctgctgc gaacaacgat aggatcggtg attttttcga
     2221 ggaattttct gatcgcgccg agtacgctgt caggaagctt cgtcgccaga aacgcttctt
     2281 cgatagtttc aggcgatcct ttcttcagtg cttcaaggtt cgggatctgc gagaccacgc
     2341 tgtcgaaaga atcggtggca ataaaaagag acggcggaaa gaccacccgc ttcagcgtct
     2401 caacgccgct gtcccggagt ttggccattg caaaaagaag ggatcgccct ttaccgccga
     2461 tactgccctt gccgacgatc aggtgtgcga acgaaggatc gtccaaaggc tcccaccgga
     2521 agtatctcct gacaatttct tcgttctccg taaacgacgc gctcttctct gatggggaga
     2581 actgtcccga tggcggaagc tgtaccgagt attcttcatt ttttcccatg tcatctctcc
     2641 ttgaagagca ttgtcgctcc ttgagacgcg agcgaccagg ttcatccggt ttcgacatgc
     2701 cggaggctga agacctgttt aatcaagaat cggaacaaaa gaaccggagc cgggagaatg
     2761 ttcctgtttc ttccgatgag cagaaagttc ccatcggtct ccttcgtcga tggacttgag
     2821 tgcgtggatt atattgtact tgattcttgg atttccggca gcaagttgat cgatgatttt
     2881 tttcgcccgc gctctttcgg tctccagaga aaaggggcag atgtagggaa gaaccgggag
     2941 ttccagccgc gagacttcct tcaagattcg ccgctcttct accaggatga gcgggcggat
     3001 gaccgtgacg ccggagcggc tttgccagaa tttcggctga aaagacttga atctgccggt
     3061 gtggaagaga ttcataagtg cagtttcgac ggcgtcgtcc aggttgtgac ccagagcgag
     3121 cacattacac ccggactcgt tcaccgccgc gttcaaaatt cctctgcgga tgttcgcgca
     3181 caggctgcaa ggtgaacgct cgtttctgat attgataata ttgacaatgg gatgtgtttt
     3241 taccgagtac gaaatgtcga gggattcaca gaacgctcgc aaagattctg tattcgtctc
     3301 tcctccggtg atatccacag tacaggcgga gagggaaaac ttgacggggc ttcttcgccg
     3361 caatccggag agcgccgtcg caagcatcaa gctgtccttc cctccggaga gaccgatcat
     3421 gattctgtct ccggaggtta tcatctggaa ctcggtaatg gcaagcccca tcttttttcg
     3481 gatcgaaaaa ggaagagtgg atgtattttt ttcggtcacc tgtgttcctc ctcgaaaaac
     3541 ggtcgatatc gtgttagaat agttcttaag tcccatgcta gggattcggc acgggaccgc
     3601 gcgtaccttt cttcactata gcataacgga gcgcgttttc gcagactcgg acggaggtgt
     3661 ccacatggag cagatacagg ttcttattgc ggatgatcac aagttattcc gcgacggtgt
     3721 taaaaagctt ctggagctgg agtcggatat cgttgtgata ggagaggccg gcgacggaga
     3781 agaagcgctt cttctcatca gggagcacga accggacatc ctgctcttcg acattaatat
     3841 gcccagagtg gacggcatcc agcttgtacg cgaattgaac aacatcccgc ataaaatgcg
     3901 gtacgttgcg atcagcgcct acgacgacga agactgtctt tccgctcttt cggcggcggg
     3961 cgttcttggg tttgtcttaa aagcctccgg aaaggttgag ctgctctccg caattcgctc
     4021 cgccgccaga ggccagccgt acgtcgaccc gcgcgttgcc ggcaagctgc tcacctcgtt
     4081 ctcgcgcagg aaagaggaga acgaccagct gtacgagctg actccccgcg agaaggagat
     4141 cctctactgg ctctctcagg gcttgagcaa taccgaggtg gccgacagaa tggttctctc
     4201 cgagaaaacg gtgaagaatc atgtgagcca tgtgctcaag aagctcgatc tccgcgacag
     4261 gacgcaggcg gctatcctcg cgtggcgtat cggcttcgcg cagctctctc ctgatacatt
     4321 ggcgcatatt ttggagagcg aagtgcgata ggaatcaaat ctgtttcctt atgcctcaag
     4381 agaacgaaaa aacagggctc cgcagttggt accgcggaac cctgttttgt gtagaggaaa
     4441 cgaaaagcct cagtatctat tttagttgcc ggaaccgccg gtcagctggc tcaatgtgga
     4501 ggagagccag cttgcctgct gctggagctt cgcaagatga acctcggcgt ttgcgaacga
     4561 ctcgtagagc ccgcgtgccc gtacttcaag ccggcgctcc aggtctgaaa tacgtttgtc
     4621 aagagtagta atttccgacc gaaaagtgta tatctggctg gcaatccgtc ccttgggtgc
     4681 agtcgtgtcg ccaacctgca cctggcttgt gtccaccatg ttgccgatat agtcatccat
     4741 gctcgtcatc atggtgttca tgatggcggc aactccttcc ggatcttcgc gcagcgcttc
     4801 catgaactta tcggtatcga attcgagctt tccgctcttg ccgtaatccg cgctctcggt
     4861 ggagatacca agttgggaca aaagcgtaaa gggcgtgccc aaaccgagag tgtcaagcac
     4921 gtcgtcggag gcggagaggg agaattttct tcccggcgac gcttcgatca ccagctggtt
     4981 gttcacaact tttgcgctgg ccatcggaat ggggtacgta cgcccctgct cgtcgtggag
     5041 catttcgtag gaagtattga tcttgcttgc gatcgtgctc agagtatctg aaggcgtgac
     5101 ttctatttgg gctgttcgca cgcccactgt gagctcgaac gtggaattcg aactcaatcc
     5161 cctcgaagcc atggtcccga gaaccgtgct tccggtcttg gtcgagtact tcgccgttac
     5221 cgagcttgtc ataagcattc gaagctgcga tttgctctgc cagagcatgg agttgccgtg
     5281 caacaatccg aacttctttg agaagtcgtc gtcggcatct tcctgcgtgc tctccgataa
     5341 gcggatgttg atccagtcga gagcgtcgtt gtacgcagtc acaaaatcct gtactccggt
     5401 cacggcttgc tccgcatcct gggtaatgtc cataatgacc gttcccgggc cgttgatcgt
     5461 cagtttgact ccttctagca aatcgtcgac ttgatttgaa gaacgagtga ttgaaagacc
     5521 atcgatcttg aatatagcat cctgagcctg gaggttgtaa tccgggtcac cgatagtact
     5581 gtcatcaaga tccagaagtg ttaaaacctc cggctctcca gtcaagctga acgcatttga
     5641 gttgaccgtg taggatacgg tataggtgac tcccgctgcg ggttcgatcc cgcccatgct
     5701 ccaatctatt acatcgcttc ccgaggtgac cgtgaagtca gtaccttccg tgtaggtatt
     5761 agtgccgtct gagatgacaa gagtcccatt ggacggtgct cctttagcga cggtgaaacc
     5821 tagtttgtcg gttgtcccgg ctcctctggt aacatcccag tccgttttcg tggttcccaa
     5881 accggtattg gagcttttta gaatgagtcg attatcaata accgttgcgg ttatacctaa
     5941 gccttcgcca taggcttctc cagtggatgg gtctatagtg gcgtctatag cggtgttgat
     6001 tttatccgcg atatcttgaa gggaatcggt tttgagcact tcgatatccg cacgtctgcc
     6061 tcctacgttg atttggaacg ttcttgtttg gtccaaaccg gcttcttcca tggtagtcga
     6121 gatctgcgtg ctgaagcggg tttctgcaac cgccttctgc gtaacatcga tctcatgacg
     6181 tgcgatcgcc gcagatgcat ccacagtcgc cgtaactatg ctgcttgcat cgacacctcc
     6241 aagagcggta aattccgcgg cttttgcctt gaagactgat tccagcttca aaggcgagac
     6301 cacgctgcga agcgacttca agcttgcgga aaactcatcg aacaatccga tcttgagctc
     6361 gagcgtatcc tg
//
LOCUS       DAOPKU010000124         6238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_13431_length_6238_cov_5.198447, whole genome shotgun sequence.
ACCESSION   DAOPKU010000124 DAOPKU010000000
VERSION     DAOPKU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13431_length_6238_cov_5.198447"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="PK849_05290"
     CDS             <1..1349
                     /locus_tag="PK849_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HPE65573.1"
                     /translation="IFVLFPILLCMSRFGKLRLGEPGSKPEFGNISWFAMLFSAGMGV
                     GLVFYGVGEPVYHFMTPPFGAAAGSAKAAEDALRTTFFHWGLHPWAGYAVIALCLAFF
                     QYRKGAPGLMSSMFLPVLGEKGNASMLGKTIDILAIFATIAGLATSLGLGTLQINSGL
                     KYLFNAPQNVTFQLVIIATLAFVYTGTAVTGIEKGIKFISNVNLFLACLLSVSLFFIG
                     PTMAIIESLMTGIGDYLSNVVNQSFHMAPYGGEYKGWLGGWTLYYWAWWIAWAPFVGS
                     FIARISRGRTIREFVSGVLVVPALGSFTWFAIFGTSGLNLELTGAAKIAEKVAADISI
                     GVFEVYTHYPLGGIMSIIMVALISTFFITSANSATFVLSMYSSQGDMNPPKFRMGIWG
                     VLMAALAFVLLMSGGLQSLQIASIAAAAPFAVIMVIACWCLWKALKKDEATFTELN"
     gene            1511..2497
                     /locus_tag="PK849_05295"
     CDS             1511..2497
                     /locus_tag="PK849_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65574.1"
                     /translation="MEYGSLLLLFDEENPCWTEELVRIAEEDPGQLALMLGEGLLAKE
                     GELFFLTGAGREAFSEEAAANYLPAVPGIPGADKEKDLFRTRLRLLLDKKHIQRWGVK
                     EFVLSARFPTPPLQDDELFTRSSVFEWIWPSSPIIGQMREDWPVTGLAARKETPPPPD
                     AAAKWFEAKGSYPAYFEADLLHLSRYDFQAYAHIPRSPNDPWGILNADRFFCLQAQAP
                     QAGNLDGFLATIGRFQLALEVLRRMVLPGYIDLDSFDQDGINWLLFLFEREEEAEACV
                     SLLAPFGLDLIRGSMPMDVWALSFEALENFKSKAETIHDLIPVIGRPVVRTP"
     gene            2712..3815
                     /locus_tag="PK849_05300"
     CDS             2712..3815
                     /locus_tag="PK849_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPE65575.1"
                     /translation="MNSMFAARMERLAEELRRREADALFVFSSELDNRPAVQYLSGFT
                     GSFAVVVAGVNGGRLVTDSRYFIQAEEESFLPLVRMEDRDPWPAVQEALRQLGVRRLA
                     VEDDKLSLERGRALENLLPSLIGTTRLVQQLRAVKDAEELELLRRSARIAAEAFEAFL
                     PAIRPGRTEAELAADLVHEIRRRGAQQMAKGHFVVASGPRGARPHGIFSDRVVQPGDF
                     ITMDFGAVYNGYVSDMTRTVGVGDVDPKLVEIYHIVREAHDRAIAAVSPDATGSDVDE
                     AARSLIRGRGYGEFFSHSTGHGIGLELHELPVVNRMNGEKLPVGSVVTVEPGIYIDGL
                     GGVRIEDDVIVTPHGAEVITSASGTELRLLSPA"
     gene            3922..5430
                     /locus_tag="PK849_05305"
     CDS             3922..5430
                     /locus_tag="PK849_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE65576.1"
                     /translation="MKKFRLIALALLAVAALALPAAAAELRIAQVADISSLDPHKVSD
                     IYSANVIRQIYSNLVQVDEHMEIVPDLAESWENPDNLTWIFHLRKGVTFHNGDPLTAA
                     AVKFSLERIKDPATASPGATHLREVASVEAIDEHTLKIVTKRPFAPMLMSLTRYETSI
                     VSERAVKEAGADYGSKAAVGTGPFKFMSWSRGDKVTLEKFDGYFRGPASLDTVVYRGI
                     PEDATRLVELESGGVELIPGNFPYQDFVTIEESGKFLTYQTPAMSTLYMGLNTEIKPF
                     DNKLVRRAMNYAVDKQAIVDAIYFGFGAPARGPLSPVIWGFDPKREPAYPYNPEKARE
                     LLKEAGFPDGFDMTIFSDNRTERRSAAELIQAYLSEVGIRAQVELMEWSALLSTSAKG
                     IGGAFMLGWTGTGDADGGMTPVYHTANIGSSNRFRYSIPALDALLEKGAATLDQTERR
                     KIYAEAQQLAVDECPLVFMISQELLGASSKKVQGFKLYPNMISPLYGVTVAK"
     gene            5504..>6238
                     /locus_tag="PK849_05310"
     CDS             5504..>6238
                     /locus_tag="PK849_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE65577.1"
                     /translation="MLRYFFKRLMFLIPVLFGVSILIFSIVHLAPGDPAEVMLGPAAT
                     QEDIANLREQLGLTKPLYVQYGIFLGNALKGDLGRSIKSNAPVMEEIMERFPATLTLA
                     LLGMTFAIVIGLPLGIIAALKQNTWVDALCSFLALVGFSIPNFWIALMLMLLLSIYIP
                     ILPSSGFDSWKNLIMPTIVLGIQTMAVIARMTRSSMLEVIRQDYVRTAKAKGISDRLV
                     LTRHILSNVMIPVVTIAGLYFGHQLGG"
BASE COUNT         1174 a         2078 c         1861 g         1125 t
ORIGIN      
        1 acatctttgt tctcttcccc attctcctct gcatgagccg cttcggaaag ctccgtctcg
       61 gcgaacccgg gtcgaagccg gaattcggca acatctcgtg gttcgccatg ctcttctccg
      121 cgggcatggg cgtcggcctc gtcttctacg gcgtcggcga acccgtctac cacttcatga
      181 cgcctccttt cggcgcggcc gccggaagcg cgaaagccgc cgaggacgcg ctgcggacca
      241 cattcttcca ctggggcctc catccgtggg cggggtacgc cgtcatcgcc ctctgcctgg
      301 ccttcttcca gtaccgcaag ggcgctcccg gactgatgag cagcatgttc ctccccgtgc
      361 tcggggaaaa ggggaatgcg agcatgctcg gcaagaccat cgacattctc gccatcttcg
      421 ccaccatcgc cgggcttgcc acatcgctcg gattgggcac gctccagatc aacagcggcc
      481 tgaagtacct cttcaacgcg ccgcagaacg tcaccttcca gctcgtcatc atcgcgacgc
      541 ttgcgtttgt gtacaccggc acggcagtca ccggcatcga gaaaggaatc aagttcatct
      601 ccaacgtcaa tctctttctc gcctgcctgc tgagcgtctc gctcttcttc atcgggccca
      661 cgatggcgat catcgaatcg ctgatgacgg gcatcggcga ctacctctcc aacgtggtga
      721 accagagctt ccacatggcg ccctacggcg gcgagtacaa ggggtggctc ggcggctgga
      781 cgctctacta ctgggcgtgg tggatcgcgt gggcgccctt cgtcggttcg ttcatcgccc
      841 gcatctcccg cggccgcacc atccgtgagt tcgtctccgg cgtgctcgtc gtgcccgcgc
      901 tcggcagctt cacctggttc gccatcttcg gaacctccgg cctgaacctc gaactcaccg
      961 gcgcggcgaa gatcgcggag aaggttgccg cagacatctc catcggcgtc ttcgaggtct
     1021 acacccacta cccgctgggc ggcatcatgt cgatcatcat ggtggcgctg atttcgacct
     1081 tcttcatcac atcggccaac tcggcgacgt tcgttctctc gatgtactcc agccagggcg
     1141 acatgaaccc gccgaagttc cgcatgggca tctggggcgt cctgatggcg gcgctcgcct
     1201 tcgtcctgct gatgagcggc ggactccagt cgctgcagat cgcctccatc gccgctgcgg
     1261 cgcctttcgc ggtgatcatg gtgatcgcct gctggtgcct ctggaaggcg ctcaagaagg
     1321 acgaggctac cttcaccgaa ctgaactgac ctctggacga accggccgtt tcgcgctacg
     1381 cttccgaagg ggaatgcgta cggctgaaga gaaagaggag aaaaacgcgt ctcgacgcgc
     1441 ccctggcatg ctccgccatc cgggggcgct ttttgtacga aaagagaaga ttcgagagga
     1501 ggaagaacac atggagtacg gaagccttct gctattgttc gacgaggaaa acccctgctg
     1561 gacggaggag ctcgttcgta tcgccgaaga ggatccaggg cagctcgcac tgatgctcgg
     1621 cgagggcctg ctcgcaaagg agggcgagct tttcttcctg accggcgcgg ggagagaagc
     1681 cttcagcgag gaggcggcgg cgaactacct gcccgccgta ccgggaattc ccggagcgga
     1741 caaggaaaag gaccttttca gaacgcgcct ccgcctgctg ctcgacaaga agcacatcca
     1801 gcgctgggga gtgaaggagt tcgtcctgtc cgcgcgcttc ccgaccccgc cgctgcagga
     1861 cgacgagctc ttcacccgct cgtccgtttt cgaatggatc tggccctctt cgccaattat
     1921 cggacagatg cgcgaggact ggcctgtcac gggactcgcc gcccgaaagg aaacgccgcc
     1981 tcccccggac gccgccgcga agtggttcga ggcgaaggga agctacccgg cgtacttcga
     2041 ggcggacctg ctccacctca gccgctacga cttccaggcg tacgcgcaca tcccccgctc
     2101 ccccaacgac ccgtggggaa tactgaacgc ggaccgcttc ttctgcttgc aggcgcaagc
     2161 gcctcaagcg gggaatctcg acggatttct cgcgacgata ggacgctttc agctcgcgct
     2221 cgaagtgctc cgccgcatgg tgctccccgg ctatatcgac ctcgacagct tcgaccagga
     2281 cggcatcaac tggctgctct tcctcttcga gcgcgaggag gaggccgaag cgtgcgtctc
     2341 gcttctcgcc ccgttcgggc tcgacctgat acgcggctcc atgccgatgg acgtctgggc
     2401 gctcagcttc gaggcgctgg aaaactttaa gtctaaggcg gagacgatcc acgaccttat
     2461 cccggtcatc gggcgccccg tcgtccggac cccctgaaga cgaagaaaaa cagtcgggag
     2521 agaagcgccg acgacgctcg tccgaggcgt ctctcccccg gagcacgctc ctgaggcttg
     2581 tccggacact tagttgacga aagaactttt ctccgagtaa aagaaaaccc tttcaccctc
     2641 atcatcgact tggtataatg catactgttc cgtatcctat ttcaaagaag gggagcggtc
     2701 gtgtgcgaga gatgaacagt atgttcgcgg cgcggatgga gcgcctggcg gaagaactcc
     2761 ggaggagaga ggccgacgcc ctcttcgtct tcagcagcga actggataac cgtcccgcgg
     2821 tgcagtacct ttcagggttc acaggctcct tcgccgtggt tgtggcgggg gtgaacggcg
     2881 ggcgtctggt cacggattcg cgctacttca tccaggccga ggaggaatcc ttcctccccc
     2941 ttgtacggat ggaggaccgc gacccgtggc ccgcggtgca ggaggcgctc cggcagctgg
     3001 gcgtgcggcg gctcgcggtg gaggacgaca agctcagcct cgaacggggg cgcgcgcttg
     3061 aaaacctgct cccctcgctg atcggcacaa cgcgccttgt acagcagctc cgcgccgtca
     3121 aggacgccga ggagctggag ctactgcggc gctcggcccg gattgcggcg gaggccttcg
     3181 aggccttcct tccggcgatc cgtccgggca ggaccgaagc ggagctcgcc gccgacctcg
     3241 tccacgaaat ccgccgcagg ggagcgcagc aaatggcgaa ggggcacttc gtcgtggcct
     3301 ccggacctag gggcgcgcgt ccgcacggca tcttctccga cagagtcgta cagccgggcg
     3361 acttcatcac aatggacttc ggcgcggtgt acaacggcta cgtctcggac atgacccgca
     3421 ccgtgggcgt cggcgacgtc gacccgaagc tcgtcgagat ctaccacatc gtccgcgagg
     3481 cgcacgaccg cgcgatcgcc gcggtttctc ccgacgcgac gggaagcgac gtcgacgagg
     3541 cggcgcggag cctcatccgc ggccgcgggt acggcgagtt cttttcgcat tcgacggggc
     3601 acggcatcgg gctggagctg cacgagctgc cagtggtcaa ccgcatgaac ggcgaaaagc
     3661 tccccgtcgg ctccgtggtg accgtcgaac cgggcatcta catcgatggc ctgggcggcg
     3721 tgcgcatcga ggacgacgtg atcgtcacgc cgcacggcgc ggaggtgatc acaagcgcct
     3781 ccgggacgga gctgcgtctg ctctccccgg cgtagaaaaa agtaccgggg ttctccccgg
     3841 tgaagcatag atagattgag aaagcactcg aagacgccgg aaaaagcggc gtcgaaaaaa
     3901 acagaaaggt cgtgatgcac agtgaaaaag ttccgtctca tcgcactcgc gcttctcgcc
     3961 gtcgcagcgc tcgctcttcc cgccgccgca gccgagctgc ggatagccca ggtcgccgac
     4021 atctccagcc tcgacccgca caaggtcagc gatatctact ccgcgaacgt gatccggcag
     4081 atctacagca atctcgtgca ggtcgacgaa cacatggaga tcgtccccga cctcgccgag
     4141 agctgggaga accccgacaa cctcacgtgg atcttccacc tccgcaaggg cgtgaccttc
     4201 cataacggcg atccgctcac cgccgccgca gtgaagttct ccctcgaacg gatcaaggac
     4261 ccggcgaccg catcccccgg cgccacccat ctgcgcgaag tggcctccgt cgaggcgatc
     4321 gacgagcaca cgctgaagat cgtcacgaag cggccgttcg cccccatgct gatgagcctg
     4381 acccgctacg agacctccat cgtaagcgag cgcgccgtca aggaagcggg cgcggactac
     4441 ggcagcaaag ccgccgtggg caccggtccc tttaagttca tgagctggtc tcgcggcgac
     4501 aaggtcacgc tcgaaaagtt cgacggctac ttccgcggcc ccgcgtccct cgatacggtg
     4561 gtctacagag gtattcccga ggatgcgacg cgcctcgtcg aacttgagtc cggcggcgtc
     4621 gaactcattc ccggcaactt cccctaccag gacttcgtaa caatcgagga gagcggcaag
     4681 ttcctcactt accagacgcc cgcgatgtcc acgctctaca tggggctgaa caccgagatc
     4741 aaacccttcg acaacaagct cgtccgccgg gcgatgaact acgcggtcga caaacaggcc
     4801 atcgtcgacg ccatttactt cggcttcggc gcaccggcca gaggccccct ctcgcccgtc
     4861 atctgggggt tcgacccgaa gcgcgagccg gcctacccgt acaacccgga gaaagccagg
     4921 gaactgttga aagaagctgg attccccgac ggattcgaca tgaccatctt ctccgataac
     4981 cgtacggagc gccgcagcgc cgccgagctg atccaggcgt acctctccga ggtcggcatc
     5041 agagcgcagg tcgagctgat ggagtggagc gcgctgctct ccacctccgc aaagggtatc
     5101 ggcggcgcgt tcatgctcgg ctggacaggc acgggcgacg ccgacggcgg catgaccccc
     5161 gtctatcaca ccgcgaacat cggctcctcc aaccgcttcc gctactccat ccccgcgctc
     5221 gacgcgctgc tggagaaggg cgcggccacg ctcgaccaga ccgagcgcag aaagatctac
     5281 gcggaggcgc agcagctggc cgtcgacgag tgtccgctcg tcttcatgat cagccaggag
     5341 cttctgggcg cgtcctcgaa gaaggtgcag ggattcaagc tctatccgaa catgatctcc
     5401 ccgttgtacg gagtgaccgt cgcgaaataa ggcggacgaa cgcgccggag cccccgcgtc
     5461 gcgcgtcggg ctccggcgat atttcccctg gaggactggt tctatgcttc gatacttttt
     5521 caagagactg atgttcctca tccccgtgct cttcggcgtc tccattctca tcttctccat
     5581 cgtccacctc gcccccggcg accccgccga agtcatgctc ggccccgcag cgacgcagga
     5641 ggacatcgcc aacctgcggg agcagctcgg cctgacgaag cctttatacg tccagtacgg
     5701 catctttctg ggcaacgcgc tcaagggcga cctgggccgc tcgatcaagt cgaacgcccc
     5761 ggtcatggaa gaaatcatgg agcgattccc ggcgacgctc accctggcgc tgctgggaat
     5821 gacgttcgcc atcgtcatag ggctaccgct tggcatcatc gcggcgctca aacagaacac
     5881 atgggtggac gccttatgca gctttttagc cctcgtcggc ttttcgatac cgaacttctg
     5941 gatcgcgctg atgctcatgc tgctgctctc catctacatc ccgatccttc cgtcgtccgg
     6001 gttcgactcg tggaagaacc tcatcatgcc gaccatcgtg ctcggcatcc agacaatggc
     6061 cgtcatcgcg agaatgaccc ggtcgagcat gctcgaagtc atccggcagg actacgtccg
     6121 cacggcgaag gcgaagggca tatccgaccg gctcgtactc acgcggcaca tcctgagcaa
     6181 cgtgatgatc ccggtggtca cgatcgcggg gctctacttc ggccaccaac tcggcgga
//
LOCUS       DAOPKU010000125         6202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_13538_length_6202_cov_5.125752, whole genome shotgun sequence.
ACCESSION   DAOPKU010000125 DAOPKU010000000
VERSION     DAOPKU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13538_length_6202_cov_5.125752"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK849_05315"
     CDS             <1..456
                     /locus_tag="PK849_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE65578.1"
                     /translation="PSPNLLLLRSMTKDYGLTGLRLGYILGATDLLRPLRALQPPWSV
                     NACAQAAGEEALRQNAYYEAQWREVRSLTERMASKLRALGYAPSPPSANFILFKVGDT
                     AALKEFLWRERILVRDCASFGLPGYVRVGVRSEAENNRLIECISLFKER"
     gene            462..1010
                     /gene="cobU"
                     /locus_tag="PK849_05320"
     CDS             462..1010
                     /gene="cobU"
                     /locus_tag="PK849_05320"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HPE65579.1"
                     /translation="MGKITFVLGGARSGKSAWAEARAFEMERSGSSVVYLATCEYRAD
                     DAEMCDRISRHRSRRPSAWRTVEEPYGIEDVVARLDAGSVALVDCLSLWVSNLLLMTP
                     EQGDSGIADSVLGRVRAFLDAAKRSSGDVIVVSNETGCGVVPSSHLGRLYRDILGWAN
                     QEAAQAADEVWFLVAGLPQRLK"
     gene            1096..2178
                     /gene="cobT"
                     /locus_tag="PK849_05325"
     CDS             1096..2178
                     /gene="cobT"
                     /locus_tag="PK849_05325"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HPE65580.1"
                     /translation="MTTLQQTIEKIRPLCAESMEAARRLQDVLTKPKGSLGRLEELSV
                     RLAGIQRTVHPVIRDKVIMTMAGDHGVVSEGVALYPKEVTVQQVLNFLRGGGGVNVLG
                     RHAGARVVLVDMGVDYDFEPMEGLQIRKVARGTASMLRRPAMTREQAVQALENGIAAV
                     YEEAVKGMDILGTGEMGIGNTTPSTAIACAVTGVAVPLATGRGAGLTDDGLKRKIEVI
                     EKSLALHRPDPKDGIDALSKVGGFEIGGIAGAILGAASLGIPVVVDGFISTAGAVVAA
                     LLCPRAKEYMIYSHNSVEIGHEHMFSFLEARPLLDLGMRLGEGTGAALAISIVEGAVK
                     VLNEMATFESAGVSGPVDSEGVADEG"
     gene            2180..2911
                     /gene="cobS"
                     /locus_tag="PK849_05330"
     CDS             2180..2911
                     /gene="cobS"
                     /locus_tag="PK849_05330"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HPE65581.1"
                     /translation="MGILEGFFRALSFLTILPCPKRESTVEEYGRASGWFPVAGAVLG
                     AILYAAALLFHRFFSPLPASALVVALWAFLTRGFHLDGLADTFDGIGGGFDRESRLAV
                     MKDSRLGTFGGTALAAALLLKTVILAEFGGRLEVLVLAPVLGRWGILIAMRYFPSARP
                     GGMGDTFRKQCGKPQLILGSLAALWAVLLLSGLSGLPLLAIVPALTFLFSLWLKRLLG
                     GLTGDSHGAVCEIVELTTLFFFAAL"
     gene            2937..3773
                     /locus_tag="PK849_05335"
     CDS             2937..3773
                     /locus_tag="PK849_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPE65582.1"
                     /translation="MFKECITSDPPRDPRFPPSAATPTILSGGEDIFGLIYTAQGKGP
                     RPTMLFLHGFPGNEKNLDLVQIFRRAGFNTMFFSYRGAWGSHGSFSFSNVLEDSLAAV
                     DFLRSDEARTQCSVDGERIVLVGHSMGGFAAIKTAAARPFIKETISLSGWNIGLDGKL
                     FPEDGAAAERVRGLVDDAVPRLAGTSSEALLREIAEKKDAFDLRDSVRSFAGRSVLLV
                     GAAHDDAVPPETHHELLLDAFRATEGVQAEGVMLDDDHSYSASRVALAKTMLEYLEKR
                     GY"
     gene            complement(3834..5396)
                     /locus_tag="PK849_05340"
     CDS             complement(3834..5396)
                     /locus_tag="PK849_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65583.1"
                     /translation="MNIPMGATMEMRFPLLQDFLPTILSCCREISGLFGFSHKDTLRL
                     EIALEEALTAIIPGESSDPLPKSLTVRFRRVPLGIRVILGEFHAPLDRKSMDAYSPGE
                     EPDERDFSGLGLFLMRNLVDQAAFYDFEAEGRGKELHLFKMLRREADEPLPQLPLAAI
                     PEKNEPAVREKPFSSERVAPSRVSAITSSFAHPGGESVESLSSIQLREALADPDISLF
                     AAWGERGELLALQALTASAQRRSLGVAFPPLFTKNAEKEACAATLLNALIEHVEDDGE
                     IEGLLGFVPGKSPAMASLFASKGFAECALLPALPGNSFLKGNVAGMASFRPCALKQIP
                     SRPGAPEDYRDVLRRIDAEEAQKAPLYAPERLHALLRLLLKEGKQPLLSHPLENQEDF
                     RKRSVISAAAEFQKQRCDLIVHDYGLDFLFQLSGLTSKLLGKDLASLTLHLPMELAAT
                     ASGAEEAEKAGYVFCGVIPGTPQGSELIYVHCKNLPELLEAAHPATDTGRQLVAWAGE
                     DCRRIRFLTNWD"
     gene            5621..>6202
                     /locus_tag="PK849_05345"
     CDS             5621..>6202
                     /locus_tag="PK849_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPE65584.1"
                     /translation="MKRIHSFLHRCKKMWFGGGGAACALAFLCCAFFPGLTGAHSAWA
                     LAGLEVPQSPMYHAASFMDEPLITAERHQELYRRLKEQFYSPWTQTAPRQATEVLEWV
                     FDRYGVNVYGENLQPRSAAWIEEQRRQARLDAVGELNRHAVSVRPTSLRLMPTETPAF
                     RSPELAGEGYPFDYLQNSAVHPGEPLFVSHLSLD"
BASE COUNT         1093 a         1819 c         2066 g         1224 t
ORIGIN      
        1 ccgtcgccga acctgctgct gcttcgttcg atgacgaagg actacggtct gacggggctg
       61 cgactcggct atatcctggg agcgaccgac ctgctccgcc cgttgagggc gctgcaaccg
      121 ccgtggagcg tgaacgcatg cgcccaggct gcgggagagg aagccctccg gcagaacgcg
      181 tactacgagg ctcagtggcg cgaggtgcgt tcgctgactg aacggatggc ctcgaagctg
      241 cgcgctcttg ggtatgcgcc gtcgcctccg tcggcgaact tcatcctctt taaggttggc
      301 gatacggcgg cgctcaagga attcttatgg agagagcgca ttctcgtccg cgactgcgcc
      361 tccttcggcc tgcccggata cgtacgggtc ggcgtgcgtt ccgaggccga gaacaaccgg
      421 ctgatcgaat gtatttcctt gtttaaagag aggtgacgtc catgggaaaa ataaccttcg
      481 tcctcggcgg cgcgcggagc gggaagagcg cgtgggccga ggcgcgggcg tttgagatgg
      541 aacggagcgg ttcttccgtg gtttatctgg cgacctgcga ataccgcgcc gacgacgcgg
      601 agatgtgcga caggatctcg cggcaccgtt cgcggcgtcc gtccgcgtgg aggaccgtgg
      661 aagagccgta cggaatagag gacgtcgttg cacggctgga cgcggggagt gtcgcgctcg
      721 tggactgcct gagcctctgg gtgagcaacc tccttctcat gacgccggag cagggcgact
      781 ccggaatcgc agattccgtt ctgggacgag tccgggcgtt tctcgacgcg gcgaaacgct
      841 cctccggcga cgtgatcgtc gtctccaacg agacggggtg cggcgtcgtt ccttcttctc
      901 accttggaag actctaccgc gacattttgg gctgggccaa ccaggaggcg gctcaagccg
      961 ccgacgaggt ctggttcctc gtcgcgggac tgccgcagcg gctgaaataa cgtagcacgc
     1021 aacggaaacg tgcgtactca cgcacgtcct tgcgcaatca tctcccgtgc acgcgggaaa
     1081 ttcttaggag gcatcatgac aactcttcaa cagacaatcg aaaaaatccg cccgctgtgc
     1141 gctgaatcga tggaggcggc gcgccgcctc caggacgtgc tcaccaagcc gaaggggagc
     1201 ctcggcaggc tcgaagaact ttcggtcagg ctcgccggta ttcagcggac tgtccacccg
     1261 gtgatccgcg acaaggtcat tatgactatg gcgggggatc acggcgtcgt ttccgagggc
     1321 gtggcgctct accccaaaga ggtgaccgtc cagcaggtgc tcaacttcct gcgaggcggc
     1381 gggggcgtga acgtgctcgg ccgtcatgcc ggagcacgcg tcgttcttgt cgacatgggg
     1441 gtcgattacg acttcgagcc tatggaaggt ttgcagatac gcaaggtggc gcgcggaacg
     1501 gcgagcatgc tcaggaggcc ggcgatgacg agggagcagg ctgttcaggc tcttgagaac
     1561 ggaatcgccg ccgtgtacga agaggcggtc aaagggatgg acatcctcgg aacgggagag
     1621 atgggcatcg gcaacaccac gccatcgacg gcgatcgcct gcgccgtgac gggcgtcgcc
     1681 gttcccctcg ccacgggcag gggagcgggg ctcaccgacg acgggctgaa acgcaagatc
     1741 gaggtcatcg aaaagtcgct cgccctgcac cgtcccgacc cgaaggacgg tatcgacgcg
     1801 ctctccaagg tcggagggtt cgagatcggc ggtatcgccg gggcgattct cggcgcggcg
     1861 tcgctgggta tccctgtggt cgtggacggc ttcatctcca ctgcgggcgc ggtggtcgcg
     1921 gcgcttctct gcccgagggc gaaggagtac atgatctact cgcacaactc ggtggagata
     1981 gggcacgagc acatgttcag cttcctcgaa gcgaggccgc tgctcgacct cggcatgcgg
     2041 ctgggcgagg gaaccggagc ggcgctcgca atttcgatcg tcgaaggcgc cgtgaaggtg
     2101 ctcaacgaga tggccacatt cgagagcgcg ggcgtctcgg gaccggtcga ctcagaagga
     2161 gttgccgacg agggctgata tgggaatcct tgagggtttt ttccgtgcgc tgagcttcct
     2221 gacgattctt ccgtgtccga agcgcgagag cacggtggag gagtacgggc gggcgagcgg
     2281 atggtttcct gtggcggggg ccgtgctggg ggcgattctg tatgccgccg cgctgctctt
     2341 ccatcgtttc ttttcgcctc tgcccgcgtc ggcccttgtt gtggccctct gggcgttttt
     2401 gacgagaggg tttcacctcg acggactggc ggacacgttc gacggcatcg gcggcggttt
     2461 cgaccgggaa tcgcggctcg ccgtcatgaa ggacagccgc ctggggacct tcggggggac
     2521 ggccctcgcc gcggcgctgc tgttgaagac ggtcattctc gcggagttcg gcggacggct
     2581 tgaggtgctt gtgctcgccc cggtgctggg gcgctggggc atcctcatcg ccatgcgata
     2641 ctttccctcc gcccgccccg gaggcatggg cgacaccttc cggaaacagt gcggcaagcc
     2701 gcagcttatc ctcggttcgc ttgccgcgct ctgggcggtg ctgctgctga gcgggctttc
     2761 aggcttgccg ctgctcgcaa tcgtccccgc cctgaccttc ctcttctcgc tctggctcaa
     2821 gcgtctcctc ggcggtctca ccggcgactc gcacggcgct gtctgcgaga tcgtggagct
     2881 gacgacgctc ttcttcttcg cggcgctcta actcagtgta taggaggcaa aaaataatgt
     2941 ttaaagagtg tattacttcc gatcccccgc gcgatcctcg tttcccgccg tcggccgcca
     3001 cgccgaccat cctgtccgga ggagaggaca tcttcgggtt gatctacacc gcgcagggga
     3061 aaggaccgcg cccgaccatg ctctttctgc acggctttcc ggggaacgag aagaacctcg
     3121 accttgtcca gattttccgg cgagccggtt tcaacacgat gttcttttcg tatcgcggag
     3181 cgtgggggag tcatgggagt ttttcgttct cgaacgtgct tgaggacagc ctcgccgcag
     3241 tcgatttcct ccggagcgac gaggcgcgaa cgcagtgttc cgtggacggc gagcgcatcg
     3301 ttctcgtcgg gcacagcatg ggcgggttcg cagcgatcaa aacagcggcg gcgcggccgt
     3361 tcataaagga aacaatctcg ctctccggct ggaacatcgg cctcgacggc aagctctttc
     3421 cggaagacgg agcggcggcg gaacgggtgc ggggacttgt cgacgacgcc gtgccgcgcc
     3481 ttgcgggcac aagctccgaa gcattgcttc gcgagattgc cgaaaagaaa gacgcgttcg
     3541 atcttcgcga ttccgtccgc tcgtttgccg ggaggagcgt tctgcttgtc ggcgctgcac
     3601 acgatgacgc cgtccctccg gagacgcacc acgagttgct gctcgatgcg ttccgggcga
     3661 cggaaggcgt tcaggcggag ggcgtcatgc tggacgacga tcactcctac tcggcgtcgc
     3721 gggtggcgct ggcgaagacg atgttggagt atctggagaa gagagggtac tgacggcttt
     3781 ttagccgttg tagcgctctt tcggccggtc tccttgcgcc ggggcttcgt ccgtcagtcc
     3841 cagttcgtaa gaaacctgat tctccggcag tcttcgcccg cccatgcgac aagctgtctt
     3901 cccgtatccg tcgccgggtg cgccgcttcc aggagttccg gcagattttt gcagtgaacg
     3961 tagatcagtt cgctcccttg cggtgttccg gggatgacgc cgcagaagac gtaccccgct
     4021 ttttccgcct cctcggctcc ggaagcggtg gctgcaagct ccatcggcag atggagcgtc
     4081 agcgaggcga ggtctttccc gagcagtttg ctcgtgagtc ccgagagctg aaagaggaag
     4141 tccagtccgt agtcgtggac gatgagatcg caccgctgtt tttggaactc ggcagcggcg
     4201 gagatgacgc ttcgcttcct gaaatcctcc tgattttcta gcggatggga aaggagcggt
     4261 tgctttccct ctttcaaaag cagccggagc agcgcgtgaa gccgttccgg cgcgtacaga
     4321 ggcgctttct gcgcctcctc ggcatcgatc cggcgaagta cgtcacggta gtcctccggg
     4381 gcccccggac gggatggaat ttgcttcagg gcgcaggggc ggaaggatgc catgcccgca
     4441 acattgcctt taagaaagct gtttcccgga agcgcgggga gcagggcgca ctcggcgaaa
     4501 ccctttgagg cgaacagcga cgccattgcg ggcgactttc ccggaacgaa tccgaggagc
     4561 ccctcgatct ccccatcgtc ttcgacgtgt tcgatgagcg cgttcaacag cgtcgccgca
     4621 caggcttctt tctccgcatt ttttgtgaag agcggaggaa aggccacgcc gagggagcgc
     4681 cgttgcgcgg aggcggtgag cgcttgcagc gcgagcagct cgccccgctc tccccatgcg
     4741 gcgaagaggg agatgtcggg gtccgccaat gcttcgcgga gttgaataga agaaagcgat
     4801 tcgacgcttt cacccccggg atgggcgaaa gaggaggtga tggcggagac gcgggaggga
     4861 gcgactcgtt cggaggaaaa gggtttttcg cgaacggcgg gctcgttttt ttccggtatc
     4921 gcagccagag gaagctgcgg gagcggttcg tcggcttccc tccgcagcat tttaaagagg
     4981 tgcagctctt tccctctccc ttccgcctcg aaatcgtaga aggccgcctg gtctacaaga
     5041 ttgcgcatca gaaagaggcc gaggccggaa aaatcccgct catccggctc ttcccccgga
     5101 gagtacgcgt ccatgctctt tctgtcgagc ggggcgtgaa actcgccgag aatgactctg
     5161 attccgaggg gaactctccg aaagcgcacc gtcagggact tcgggagggg gtcggatgat
     5221 tctccgggga tgattgccgt gagcgcctct tccagggcta tttcgagccg cagcgtatct
     5281 ttatgtgaaa acccgaagag gccgctgatt tcgcggcagc aggagaggat cgtcggcaga
     5341 aagtcctgga ggagagggaa gcgcatctcc atcgtggcgc ccattggaat gttcatcgcg
     5401 cagctccttt caaaagaggc ggtatcgaga gtctatctgt accgttcggt tcaagtgctc
     5461 agtatgtcat atcgaaaaga ttctttcaac actccgcagt tcgtgaaaac gcgggcaggg
     5521 attgcgtctg gacgtccgcg gaaaaaagcg gtactcttga cggcgcgtgg tccctgcgaa
     5581 gagtgaggag ggcgccgcaa tccggaggga ggtcgtttcg gtgaaaagaa tacactcatt
     5641 tctccatcgc tgcaaaaaaa tgtggttcgg gggcggaggc gcggcgtgcg cgctcgcgtt
     5701 cctgtgttgc gccttcttcc ccggcttgac tggcgcgcat tccgcgtggg cgctcgccgg
     5761 tctggaagtg ccgcagtcgc cgatgtacca tgcggcttca ttcatggacg aaccgctgat
     5821 cacggcggaa cgtcatcaag agctgtaccg tcgtctgaag gagcagttct attcgccgtg
     5881 gacccagacc gcgccgcgcc aggctaccga ggtgctggag tgggtattcg acaggtacgg
     5941 cgtcaacgtc tacggtgaaa atctccagcc gcgttcggcc gcatggatag aggaacaacg
     6001 ccgccaggcg cggctcgatg ccgtcggaga gctgaaccgg cacgcggtga gcgttcgccc
     6061 gacgtcgctt cgtctgatgc ccacggagac gccggctttc cgctcccccg aactggcggg
     6121 ggaggggtac ccgttcgact acctgcagaa cagcgcggtg cacccgggag agccgctctt
     6181 cgtctcgcat ctctcgctgg ac
//
LOCUS       DAOPKU010000126         6168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_13649_length_6168_cov_4.547522, whole genome shotgun sequence.
ACCESSION   DAOPKU010000126 DAOPKU010000000
VERSION     DAOPKU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13649_length_6168_cov_4.547522"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2399
                     /locus_tag="PK849_05350"
     CDS             <1..2399
                     /locus_tag="PK849_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHLP bacteriocin export ABC transporter
                     permease/ATPase subunit"
                     /protein_id="HPE65585.1"
                     /translation="LAPGSGLFALPPNCWIVPLEQSVVRTTPTEELLRRGEFASVFDA
                     FCEMIFSSLAMNARLRAADDLGALRQMQKSSRKALSSALGTLGEALSGKRRKVAASRE
                     DSPLRRAFSTVAAFLGRSVAAPKGEELASERGESLRDLARASNLQLRRVTLAGPWWIS
                     DGAPLIAYVQENAEKSSGGGEMSGTPVALLPSGRGYRLVDDEGERRVDERIAATLGQD
                     AIQVYRPLPGSPLGGMDLLRFGLAGTKRDMGAVLVISVCLALLGMISPEVNRQIFGEV
                     IPQAERGRMLQLFGILVSVALSSGLLHLANGTAFLRTESVMDHDVSAGIWDRLLRLPV
                     GFFRKTTSGDLANRALGLFMLRETASRTVKTLLVQSVFLIFFLGQSFWYDWRLALSGL
                     ACLFVPAALTVVVNVVQLRFQRKMIGLQNRLLALTFQIMTGISKIRVAGAEERSFARW
                     AELFGAQRTLGTSARRLEVFLNTVMALFPLSVAIVILFALVRWSAADASYETGRFMAF
                     WSAFGALQGAFFQMVSSVTTTLNMLPMVENLAPILQAEPETGDLKTDPGEITGRIDLE
                     GVVFRYLKEGPPVLKGLDLHIEPGEFVAVVGPSGAGKSTLIRLLLGFDQPESGGIFYD
                     NKDIAELDLGKLRRQIGVVLQSGGLLSGDIASNVRCSRNMTVEQIEDALRTAGMEEDI
                     AAMPMGIHTVITDGANTLSGGQKQRLLIARAVAGKPRVLIFDEATSALDNRTQAMISR
                     SLESIQATRIVVAHRLSTVIGADRICVLEDGRLVEEGKYDELMAKDGLFAELVRRQTV
                     "
     gene            complement(2450..5503)
                     /locus_tag="PK849_05355"
     CDS             complement(2450..5503)
                     /locus_tag="PK849_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE65586.1"
                     /translation="MSMTEIVLNALLNLFALRAASAPSNLRGAVRKRVGFYLREHLGL
                     DAIGSYLELYDLTLDLQNEAPSELLLSRSAAVASGLRTQLPRDEQITILAMLLEAAPE
                     DAPSENEVLRAVAKAFAVERQFLVGYALFSGASFPWDGAGLDDRFLLPTDAPPQETRS
                     SPFRILRRPQFKRAFAVFRAEEEGPWFIRASSRGALFLDGIPFDGGIRRLRQGSVVSN
                     RQRNRIHFGEIAAAFSALSATARPHILEGDHLDFRYDGAPDTGLHDFSFSVRGGELVA
                     IMGGSGSGKSTLLSLLTGKLRPRSGRLLLDGRDVAGDAMLRRGILGYVPQDDLLFEDL
                     TVFENLYYSARLGLPDLPDAEVRRRCERMLDGLNQLDVAHIRVGSPLDKTISGGQRKR
                     LNIALELIREPAVLLVDEPTSGLSSADSLHVVSLLKAQAAAGRLVVAVIHQPSSDVYK
                     MFDRLWMLDTGGRPIYDGNPLDALVHFRSASYRAGEQEYACPRCGNVNPEQLFEIVEE
                     KTVDERGFPTKQRKVSPEQWRLLHLKTTLPAALPAPPTPENDETNLTPPPLREQFAVF
                     FLRTMKSRLANRIYRVSALLQPLLLALAAGLLFRGSWGGEYVFRNNNNLPGYFFISTV
                     IAVFLGMALSADEINRDRKILERERLLRLSRGAYLASKFAWLLLAAALQTGIYTLLGN
                     GLLHIPSSGFATWAILFSTAYCAAMIGLILSDTLSSSSAIHILIPVLLAPQIMLGGPT
                     IPFDELLRKDAGNRNVPLIAEIMPTRWGYEALMVEHYRNNPFQKTFFHDETREKWFDF
                     LTGVYIPEVRGLASYPFSPTLDIAGIPADADKAGRRLKILRNELDYLERFTGLQWRGE
                     GLNDGDLVLERYTREVQRKVMARLKEAEAVLKPLREETAQRRVATEDALRGMLGHKGF
                     EEFKNRSFNREVEKQLLGISAEDAIVISGPRLVPKVLPVVMEPESRFGRAHLFAPFKR
                     LGDFKVQTPFFNIGVLWLLSALLAAALRTRLVPRAATLFRRFTGR"
     gene            complement(5595..>6168)
                     /locus_tag="PK849_05360"
     CDS             complement(5595..>6168)
                     /locus_tag="PK849_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65587.1"
                     /translation="EQLYVLWGVGAMNISYALLFDKNPEGVWEGGREIDERIGFPRAE
                     LREESLAFAKSREGQASSSVDFIHAMLHRAKTDQAVLNTLVGGFYGSTLELFYLFSSL
                     GLASGVTEEFVQFVNAHLPQLTIAQEMLKAYSENGELAQLLDTGNRKAVIDSIVDIIT
                     KDMGKLTEDDLKQILSIVKAVRDPLTVPCN"
BASE COUNT         1089 a         1872 c         2029 g         1178 t
ORIGIN      
        1 accttgcgcc gggaagcgga ctcttcgccc ttcctccgaa ctgctggatc gttccgctgg
       61 agcagtccgt agtgcggacg accccgacgg aagagctgct ccgcaggggc gagttcgcct
      121 ccgttttcga cgcattctgc gagatgatct tctcctcgct ggcgatgaac gcccggctcc
      181 gggccgccga cgaccttggg gcgctccggc agatgcagaa atcaagcagg aaagctcttt
      241 cctccgctct tggaacgctg ggcgaggcgc tttcgggaaa acgcagaaaa gtggccgcct
      301 cgcgtgagga ttcgccgctc cggagggcct tttccacggt cgccgcgttt ttggggcgga
      361 gcgtcgccgc tccgaaagga gaggaactcg cctccgaacg cggggagtcg cttcgcgacc
      421 tcgcgcgcgc ttccaacctc cagctccgtc gcgtgacgct cgccggtccc tggtggatct
      481 cggacggtgc gccgctgatc gcctacgtac aggagaatgc ggaaaagtct tccggagggg
      541 gagagatgag cgggacgccc gtcgcgctcc ttccgtccgg acgcggatac cgccttgtcg
      601 acgacgaggg agagcgtcgt gtcgacgagc gaatcgccgc gactctcggg caggatgcaa
      661 tccaggtcta ccgtccgctc cccggctcgc cgctgggggg catggatctg ctccgtttcg
      721 gccttgccgg gacgaagaga gacatgggcg ccgtcctcgt catttcggtc tgcctggccc
      781 ttctggggat gatctcccct gaggtgaacc gccagatctt cggcgaagtc attccgcagg
      841 ccgaacgggg aaggatgctc cagctcttcg gtattctcgt ctccgtcgcg ctttcgtcgg
      901 gcctgctcca cctggcgaac ggaacggcct ttctccgcac ggagagcgtg atggaccacg
      961 acgtttcggc gggaatctgg gaccgcttgc ttcgcctgcc ggtgggcttc ttccggaaga
     1021 cgacgtccgg cgatctcgcg aaccgggcgc ttggtctctt catgctccgc gagaccgcgt
     1081 cgagaaccgt caagacgctg ctcgtccaga gcgttttcct gatcttcttt ctggggcagt
     1141 cgttctggta cgactggagg ctcgccctct ccggcctggc gtgcctgttc gttccggccg
     1201 cgctgaccgt ggtggtgaac gtcgtgcagc ttcgcttcca gcgcaagatg atcgggctgc
     1261 agaacaggct tcttgcgctg accttccaga tcatgaccgg catatcgaag atccgggtgg
     1321 cgggcgccga agagcgctcc ttcgcacgat gggcggagct gttcggggcg cagcggacgc
     1381 tggggacttc ggcgagacgc ctggaggtct ttttgaacac ggttatggcg ctctttcctc
     1441 tctccgtcgc catcgtcatt ctctttgccc ttgtccgctg gagcgccgcg gacgcttcgt
     1501 acgaaacggg gcgtttcatg gccttctggt ccgccttcgg cgcgctgcag ggcgctttct
     1561 tccagatggt aagttcggtc accactacgt tgaacatgct cccgatggtg gagaacctcg
     1621 cccccatcct ccaggccgag cccgagaccg gcgatctgaa gaccgatccc ggagagatca
     1681 cgggacggat tgacctggaa ggggtggtct tccgctacct gaaggaaggg cctcccgtgc
     1741 tgaaggggct cgacctccac atcgaaccgg gggaattcgt cgccgtggtg ggcccatccg
     1801 gggccggcaa gtccaccctc atccgtctgc tgctcgggtt cgatcaaccg gagtcggggg
     1861 gcatcttcta cgacaacaag gatatcgccg agctggacct ggggaagctg cggcggcaga
     1921 tcggcgtcgt cctccagagc ggcgggctgc tctccgggga tatcgcctca aacgtccgct
     1981 gctcgcgcaa catgacggtc gagcagatag aagatgcgct gcggacggcg gggatggagg
     2041 aggatatcgc tgcgatgccg atggggattc atacggtcat cacggacggc gcgaacacac
     2101 tctccggagg gcagaagcag cgccttctga tcgcccgcgc cgtcgccggg aagccgcgcg
     2161 tcctcatctt cgacgaggcc acgagcgcgc tggacaaccg gacgcaggcg atgatcagcc
     2221 gcagcttgga gagcatccag gcgacgcgca tcgtcgtggc gcaccggctc tccacggtca
     2281 tcggcgccga ccgtatctgc gtcctcgagg acgggcgtct tgtcgaggag gggaagtacg
     2341 acgaactgat ggcgaaggac ggtctcttcg ccgaactggt ccgaagacag acggtgtaga
     2401 agaaaacgga ctgaaggcat tcctgcggct cgtttttggg gacgtggcgt tatctccctg
     2461 tgaatctccg gaagagcgtt gccgcgcgcg gaacgaggcg cgtccgcaga gccgcggcca
     2521 gaagtgcgga gaggagccag aggacgccga tattgaaaaa cggcgtttgt accttgaaat
     2581 ctccgagccg tttgaacgga gcgaaaaggt gcgccctgcc gaagcgcgac tcgggctcca
     2641 tgacgacggg gaggactttc gggacgaggc gcggcccgga tatgacgatc gcgtcctcgg
     2701 cggagatgcc gaggagctgt ttttcgacct ctctgttgaa actcctgttc ttgaactctt
     2761 cgaacccctt gtgtccgagc attccgcgaa gcgcgtcttc ggtcgcgacc cggcgttgcg
     2821 cggtctcttc gcggaggggt ttgagaactg cttccgcctc tttgagccgc gccatcacct
     2881 tccgttggac ctcgcgagtg tacctctcca aaacgagatc gccgtcgttc agtccctcgc
     2941 cccgccattg caggcccgta aagcgctcca gataatcgag ctcgttccgc agaattttca
     3001 gccgtcggcc cgctttgtcg gcgtccgcgg ggatcccggc tatgtcgagc gtcggagaga
     3061 acgggtagct cgccaggccg cgtacctcgg ggatgtacac ccctgtgagg aaatcgaacc
     3121 acttctcccg cgtctcgtcg tgaaaaaacg tcttttggaa cgggttgttc ctgtagtgct
     3181 ccaccatgag cgcctcgtac ccccaccgcg tcggcatgat ctccgcgatc aggggaacgt
     3241 ttcggtttcc ggcgtccttg cggagcagct cgtcgaaggg gatcgtcggc cctccgagca
     3301 tgatctgcgg cgccagcagc acggggatga gaatgtgaat ggccgaggac gaggacagcg
     3361 tgtccgacag gatcagcccg atcatggccg cgcaatatgc cgtgctgaag aggatagccc
     3421 acgtggcgaa ccccgacgag gggatgtgga gcaagccgtt gcccagaagc gtgtagattc
     3481 ctgtttggag cgccgccgcg aggaggagcc acgcgaactt ggaggcgaga tacgcgcccc
     3541 tgctcaatcg gagcaggcgc tcgcgttcga ggatcttcct gtccctgttg atctcgtccg
     3601 cgctgagggc catcccgaga aagacggcga tcaccgtgct gatgaagaaa tatcccggaa
     3661 ggttgttgtt gttcctgaag acatactcgc ccccccatga acctctgaag aggaggcctg
     3721 cggccaaagc gagcagcagc ggctggagga gcgccgaaac gcggtagatc ctgttcgcga
     3781 gacgactctt catcgtccgc agaaagaaga cggcgaactg ctcgcgcagc ggcggagggg
     3841 tgagattcgt ttcgtcgttc tccggcgtcg gcggcgcggg caatgcggcc gggagcgttg
     3901 ttttcagatg aaggaggcgc cactgctcgg gggagacctt ccgctgtttc gtcggaaacc
     3961 cccgctcgtc gacggtcttc tcttcgacga tctcgaaaag ctgctccggg ttgacgttcc
     4021 cgcagcgggg gcaggcgtac tcctgttcgc ccgcgcggta ggaggcgctt cggaaatgga
     4081 cgagcgcgtc caaggggttg ccgtcgtaga tgggacgccc tccggtgtcg agcatccaga
     4141 gtctgtcgaa catcttgtag acgtccgacg atggttggtg gatcaccgcg acgaccagtc
     4201 tcccggcggc ggcttgggcc ttgagcagcg agacgacatg cagcgagtcc gcggaggaga
     4261 gcccgctggt gggctcgtcc acgaggagca ccgcaggttc cctgatgagt tccagagcga
     4321 tgttgaggcg ttttctctgc cctccgctga tggtcttgtc caagggagat ccgacacgga
     4381 tatgggcgac gtcgagctgg ttcaggccgt cgagcatccg ttcgcagcgg cgccggacct
     4441 ctgcgtccgg aaggtccggc agtccgagac gcgcgctgta gtacagattt tcgaagacgg
     4501 tgagatcctc gaagagaaga tcgtcctgcg gaacataccc cagaatgcct cgccggagca
     4561 tcgcgtcgcc tgcgacgtcg cgtccgtcga gcagcagcct cccggagcga ggccgaagct
     4621 tgcccgtgag cagcgagagc agcgtcgatt ttccggagcc gctccctccc attatcgcga
     4681 caagctcgcc tccccgcacg gagaagctga agtcgtgaag gccggtgtcg ggggcgccgt
     4741 cgtagcggaa gtccagatga tctccctcga ggatgtgggg gcgggccgtt gcggagaggg
     4801 cggagaacgc ggcggcgatc tcgccgaagt ggatgcggtt ccgttgccgg ttcgagacga
     4861 ccgatccctg tctcaggcgg cggataccgc cgtcgaaggg aatgccgtcc agaaacagtg
     4921 ctcccctgga agaggccctg ataaaccacg gcccctcttc ctccgcgcgg aagaccgcga
     4981 acgccctttt gaactgcgga cggcggagga ttcggaacgg agaggagcgc gtctcctgcg
     5041 ggggggcgtc cgtagggagg agaaacctgt cgtcgagtcc ggccccgtcc cacgggaatg
     5101 aagcgccgga gaagagcgcg tagccgacca gaaattgtcg ctccacggcg aacgccttgg
     5161 ctacggcgcg gagcacctcg ttttcggaag gagcgtcttc aggagccgct tccagcagca
     5221 tcgcgaggat cgtgatctgt tcgtcgcggg gaagctgggt tctcagcccg gaggcgactg
     5281 cggcgcttcg tgaaagcaag agctcggaag gcgcttcgtt ctgcaggtcg agcgtcaggt
     5341 cgtacagctc caaataggag cctatggcat cgagcccgag atgttcgcgc agatagaaac
     5401 ccacgcgttt ccggaccgcg cccctgaggt tcgagggagc cgacgcggcc cgtaaggcga
     5461 agagattcag cagcgcgttc aagacgattt cggtcatcga catggcttag tctccttggc
     5521 cgaagtgaag agcagtgttt tcggcgttgc ccggttccgc cggagcgtcc gcgaaggacg
     5581 ccccggcgcg tggatcagtt gcaggggacg gtcagaggat cgcggacggc tttgacgata
     5641 gaaagaatct gcttcaggtc atcctccgtc agcttcccca tatccttcgt gatgatgtcc
     5701 acgatggagt cgatgacggc ctttcgattt cccgtgtcga gcagctgcgc caactctccg
     5761 ttctccgaat aagccttgag catctcctgc gcgatggtca gctgcggaag gtgggcgttg
     5821 acgaactgaa cgaactcctc cgtgactccg gaagcgaggc cgaggctgga gaagagatag
     5881 aaaagctcca gcgtgctgcc gtagaagcct cccacaaggg tgttgagcac ggcctgatcg
     5941 gtcttcgccc tgtgcagcat ggcgtggatg aagtcgaccg aactgctcgc ctgcccctcg
     6001 cggctttttg cgaacgcgag gctttcttct cggagttccg ccctggggaa gccgattcgt
     6061 tcgtcgattt cccttccgcc ttcccaaacc ccttccggat ttttgtcgaa gagcaacgca
     6121 tagctgatat tcattgcgcc gacaccccag agaacgtaga gctgttcc
//
LOCUS       DAOPKU010000127         6157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_13688_length_6157_cov_4.908391, whole genome shotgun sequence.
ACCESSION   DAOPKU010000127 DAOPKU010000000
VERSION     DAOPKU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13688_length_6157_cov_4.908391"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PK849_05365"
     CDS             complement(<1..547)
                     /locus_tag="PK849_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65588.1"
                     /translation="MICGKRTAVRSLSILGLFLLLSVRTVYAEQAMLVGSDAGRTYHV
                     LCLDAGAPYIFRAENGDLQGVYVDILSALGEREGLKLSLSLGDTHDARRGIHLSRIDF
                     IVGTPYPVPADSELFTFFSVKNMDTADTRLIDDFELMKHRLSGKEFASYCFSLPFFEE
                     PYTLFAAGSEALPLSSLRGKIL"
     gene            complement(534..2129)
                     /locus_tag="PK849_05370"
     CDS             complement(534..2129)
                     /locus_tag="PK849_05370"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPE65589.1"
                     /translation="MKHFFSRITVLLLLLVISSDPSYGCTGILVGREASEDGSVLNSQ
                     TADGWYDSNLRVVPGETFPEGEDVSVYFGLLGDEPLPPFELGRIPQASTTYAYFRTAY
                     SCFNEHQLSIGESTIGQKDMLKAFPGEGGALLTVEQLMILALQRCKTSREAVLLIGEL
                     AERYGFLGSCANEGESLSIADPYEAWIMELLSVGFDWKPGSAPGAIWVARRVPDSHVA
                     ILCNASRISLVDENSEDFLFSKGYKEPALRHGWYDPESGAPFNWRKTYAPESGPWSPS
                     SMWVRGRLHAIHKRLLPSRSWDPYAETDSYPFSFAPEKPLSVKGIMEIFRSTMEGTPF
                     CMEDNPAWYVPRGNRELVRSAKATPFPDRFMRDLLNIPYTRPVAAKTSYSFITQSRSW
                     LPSPLGGVLWYSLDTPHFSTYAPVYAGVRSVPESWSNFKKDGFTLDSARWAVLLSSSL
                     TNSNYQRSMPTLKSLRDPLEKKTFHDFLQWEKEGSHLLLHSSPELQEWMDAITAKKME
                     EIHQTYRSIAQALIMEATIIDLW"
     gene            complement(2237..3970)
                     /locus_tag="PK849_05375"
     CDS             complement(2237..3970)
                     /locus_tag="PK849_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPE65590.1"
                     /translation="MIYSDFDPKPTFRQFKRLIGDGEVGGKARGIAFAFNAVTGSPLE
                     SVVDFPDVNFVVTTEGFDEFVSDNGIETLLREHAASEEENCDEDTSQELFDRLSAVFQ
                     SGRIRSSLLQDLEGALESIGDFPLAIRSSSILEDSRKLSFAGKYGTRFSANIGSVEDR
                     RDLLANALKDVWASLYNPAARAYRKKHGLVDSDESMAVVIQPVIGRRHNGMYYPEIAG
                     TAFSKVYRRPSTRIKKENGVMRFCFGLGTRTVDRLKANISYLSHPMLRPQGNLPADIA
                     MTSQSEFDYIDRTSGTFMTGLLSEHISFILKEHKLASSFIEIFSDNVLYWAGSDQLSN
                     GKPIFSFSNFPRRHPKFFNIVKDLSAFLEEKMGMPADMEFTYDTESEKLTLLQLRPLA
                     SYEEMARVEIPEVREEKIILMGNKMVSNGKMQNIHHLVFVDPELYGKDATFHEVAREI
                     GRINHKLSGTDYILVGPGRWGSTNPKLGVPVRYNEICNCGCLVEVGILESDYTPELSY
                     GTHFFLDLDVDGTLYLPVFDGMKGNLFNRTWLDSSFYERKRHPAVRHYTGDFSVFMDG
                     EKEIGAVISND"
     gene            complement(4008..5309)
                     /gene="asnS"
                     /locus_tag="PK849_05380"
     CDS             complement(4008..5309)
                     /gene="asnS"
                     /locus_tag="PK849_05380"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPE65591.1"
                     /translation="MTALWVSIKDLPSHVEETVVIRGWLYNLRSSGKIHFLQLRDGSG
                     TVQGVMVKKEVSEDLFAAAKGLWLEASVEVTGTVRKDERAPSGVELSVTDLRVFQNPS
                     EEYPIGKKDHGIDFLLDHRHLWLRSSRQQAIMRIRDRVIRSAREYLQENGFLLIDSPI
                     ISGAAGEGVSGLFEIDYFDSKAYLAQTGQLYAEAAAAAFGKVYCFGPTFRAEKSKTRR
                     HLTEFWMLEPEVAFYDHKDNMALQEGLICHIVAKVLEHCRTELASLERDVSKLENIKA
                     PFYHLQYDEAIEMLHKLGSDTPYGDDLGNEDETILTQQFDRPVFIECYPKTVKAFYMK
                     EHPDNPNLVLCDDLLAPEGYGEIIGGSQREDDYDRLIARIREQGLPEEPYEWYLDLRK
                     FGTFVHSGFGMGIERVVAWISGLQHIREAIPWPRTIYRLKP"
     gene            5405..>6157
                     /locus_tag="PK849_05385"
     CDS             5405..>6157
                     /locus_tag="PK849_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65592.1"
                     /translation="MNIPRLVLAEERREGKVAQSILVASVLKSLGYPLRLFCAGVDEH
                     LVRFLKLVTEQDVTVLDPFSCGSMRNFKLLFQRAASPDTLNIIFGSIGERRDENTFPV
                     HPLPGEIARILECPLVTMLYADSSAAVTSRILENVFNQISAQGVITSAVIFSSVLNPR
                     EYQLLETEAGRRVPLLCLGFIPKTLEKNNPPLMELCLQDMAQRAAFPVKAATAQLLGM
                     VDQVDWNVLWGIAKQCKECVAMDDPFKGKASGM"
BASE COUNT         1463 a         1560 c         1669 g         1465 t
ORIGIN      
        1 gcagtatttt ccctctcaag ctgctaaggg ggagggcttc gctcccggcg gcaaagagcg
       61 tgtacggctc ttcgaaaaaa gggagagaaa aacagtaaga agcaaactcc ttgccggaaa
      121 ggcgatgttt catcagttca aagtcatcga taaggcgagt gtccgctgtg tccatgttct
      181 tcaccgaaaa aaaagtaaaa agttccgagt ccgcgggaac agggtagggg gttcctacga
      241 tgaaatcaat ccgggagagg tggatgcccc ggcgggcatc gtgtgtatcg cctagggaga
      301 gggagagctt caacccttct ctctccccca gagcggacag tatgtccaca tagactcctt
      361 gaagatctcc gttctccgcc ctgaagatat agggggctcc tgcgtccagg cacaagacat
      421 ggtacgtgcg ccccgcgtcg cttcctacca acatcgcctg ttcggcgtat acggtacgga
      481 cggagagcag gagaaaaagt ccgagaatcg agagtgaacg aacggccgtt cttttaccac
      541 agatcaataa ttgttgcctc cataataaga gcctgcgcaa tgctcctgta cgtttggtgt
      601 atttcctcca tctttttcgc ggtgatggca tccatccatt cctgaagttc aggtgaggag
      661 tgaaggagca aatgggaacc ctccttttcc cattggagaa agtcgtggaa cgtctttttt
      721 tccagaggat cgcgcagtga tttcaacgtc ggcatcgacc tctgatagtt gctgttggta
      781 agtgatgacg aaagaagcac cgcccaccgc gccgaatcaa gagtgaatcc atcttttttg
      841 aagttcgacc agctttccgg cacactgcgc actccggcgt acacgggggc gtatgtgctg
      901 aaatgcgggg tgtcgagtga ataccagaga acgcctccga gcggagatgg aagccatgaa
      961 cgggattgtg tgataaagct gtaggacgtt tttgccgcga ccgggcgtgt gtaaggaata
     1021 ttcaagaggt cgcgcatgaa cctgtcgggg aaaggagtgg cttttgcact gcggacaagc
     1081 tcccggtttc ctctagggac ataccatgcg gggttgtcct ccatacagaa cggggtgccc
     1141 tccatagtcg agcgaaagat ctccattatc ccttttacgg agagcggttt ttcaggagcg
     1201 aaagaaaacg gataggagtc ggtctcagcg taggggtccc agcttcttga aggaagaaga
     1261 cgcttatgaa tagcgtgcaa gcgccctcgg acccacatcg aagaaggcga ccaggggccg
     1321 gattccggcg cataggtctt tctccaattg aagggggcgc cggattccgg atcataccag
     1381 ccgtgacgga gagcgggttc cttgtaccct tttgaaaaaa gaaaatcctc gctgttctca
     1441 tcaactaggg agatacggct tgcgttgcac agaatagcca catgagaatc cggcactctt
     1501 ctggcaaccc agattgcgcc gggagcggag cctggtttcc agtcaaatcc gacgctcagc
     1561 agctccatga tccacgcttc atacggatcc gcgatggaaa gcgactcgcc ttcattggcg
     1621 catgaaccga gaaaaccgta tcgctcggca agctccccga tgaggagaac ggcttcacgg
     1681 gatgttttac accgctgaag ggccagaatc atgagctgtt cgacggtgag aagagcccct
     1741 ccttctcccg gaaaggcttt gagcatgtcc ttctggccaa tggtggattc tcctatagag
     1801 agctggtgct cattgaaaca tgagtaggcg gtcctgaaat aagcgtaggt tgtagatgcc
     1861 tggggtatac gccccagttc aaagggggga agaggctcgt cgccgagcag tccgaagtag
     1921 actgatacat cctccccttc cgggaaggtt tcccccggaa caacccgaag attggaatcg
     1981 taccatccgt ctgcggtttg tgagttgaga acggaaccgt cttcgcttgc ttcgcgcccg
     2041 acgaggatgc cagtgcatcc gtaggaagga tccgaactga tgaccaaaag taggagcaat
     2101 acggtgattc tggaaaaaaa atgtttcatt tggaagcgac tcctctacat agttcgtcag
     2161 tgcagtcatt gggagtagaa gaagagatac ggaatggccg gccgttccgc agaaaatggc
     2221 gaaacggccg ggtgggttaa tcgtttgaaa taactgcgcc gatctctttt tctccgtcca
     2281 tgaagacgga gaagtcccct gtgtagtgcc gcaccgcagg atggcgtttg cgttcgtaga
     2341 acgaagaatc cagccaggtc cggttgaaaa gatttccttt cataccatca aatactggaa
     2401 ggtagagcgt tccgtcgaca tcaagatcca ggaaaaaatg cgttccgtag gaaagttccg
     2461 gggtgtaatc gctctccagg ataccgacct ccacaaggca accgcagttg cagatttcgt
     2521 tatagcgtac cgggacgcca agtttggggt tggtgcttcc ccatcttccg ggcccgacaa
     2581 gaatgtagtc ggttccggag agcttgtgat tgatgcgtcc gatctctctc gccacttcgt
     2641 ggaaagtggc gtctttgccg tacaattcag gatccacaaa gacgagatgg tgaatatttt
     2701 gcattttccc gttgctcacc attttgttcc ccataagaat aatcttttct tcgcggactt
     2761 cgggtatctc cacacgagcc atctcttcgt aagaggcaag gggccggagc tggagaagcg
     2821 tcagcttttc gctttccgta tcgtaggtga attccatgtc ggcgggcatg cccatctttt
     2881 cttcgaggaa ggcggaaagg tccttcacga tattgaagaa cttcgggtgc cttcttggaa
     2941 aatttgaaaa agagaaaata ggctttccgt tgctcaactg gtccgaacct gcccagtaga
     3001 gtacgttgtc ggagaagatc tcgatgaagg aagaagcgag cttgtgttct ttcaggatga
     3061 aagaaatatg ctcagagaga agaccggtca taaaggttcc ggacgttctg tcgatgtaat
     3121 cgaactcgct ctgacttgtc atcgcaatat ccgccggcag gtttccctgc ggccggagca
     3181 tcgggtggga gagataggag atatttgcct tcaggcggtc cactgtccgc gtcccgagac
     3241 cgaagcagaa tcgcatgacc ccgttttcct ttttgatcct cgttgaagga cgccggtaca
     3301 ctttggaaaa ggctgttccg gcaatttcag gatagtacat cccattatgc cggcgtccta
     3361 tcacaggctg aataacgaca gccatggatt cgtcggagtc tacgagtccg tgtttctttc
     3421 tgtaggcccg cgcggcagga ttgtacagtg aagcccaaac atccttgagc gcattggcga
     3481 gaaggtctct gcgatcttcc acgctgccga tgttcgccga aaaacgtgtg ccgtactttc
     3541 cggcgaagga gagcttgcgc gagtcctcca gaatggagct tgagcggatc gcgagtggga
     3601 aatcgccgat cgattcaagc gcgccttcaa gatcctgcaa cagggaagaa cgtattctgc
     3661 cggactggaa aacggcggaa agtctgtcga aaagctcctg ggatgtgtct tcgtcgcagt
     3721 tctcctcttc tgaagcagca tgttcgcgca aaagggtttc tattccgttg tcgctgacaa
     3781 actcgtcgaa accttccgtt gtcacgacga aattcacatc cggaaaatcc acgacagact
     3841 ctaaagggct tccggtaaca gcgttgaaag cgaaggcgat tcctctcgcc tttcctccta
     3901 cttcgccgtc gccgatcagc cgtttgaact ggcggaacgt tggttttggg tcaaagtccg
     3961 agtaaatcac ctgagtatgc ctccccgaaa tgggaaaata acgaacgtca gggcttcaaa
     4021 cggtaaatgg tgcgaggcca tggaatagcc tcacggatgt gctgcaatcc gctaatccac
     4081 gccacgacgc gctctattcc cattccgaat ccgctgtgaa cgaacgtgcc gaatttcctg
     4141 aggtcaagat accattcgta gggttcttca ggaagccctt gctccctgat tcttgcgata
     4201 agtctatcat aatcgtcctc tctttgagaa ccgccgatga tctcgccgta cccctcgggg
     4261 gcaagaaggt cgtcgcagag gacaaggttc gggttgtcgg ggtgctcttt catatagaat
     4321 gccttcacag tctttggata gcattctata aagacgggtc tgtcgaactg ctgggtgagt
     4381 atcgtctcgt cttcgttgcc gaggtcgtcg ccgtaagggg tgtcgctccc gagtttgtgc
     4441 agcatttcta tggcttcgtc gtattgaaga tgatagaacg gcgcctttat attctcaagc
     4501 ttggagacgt cgcgttcaag cgaggcgagc tccgttctgc aatgttcgag tacctttgcg
     4561 acaatgtgac atatcagccc ttcctgaagc gccatgttgt ctttgtggtc atagaaggcg
     4621 acttccggtt cgagcatcca gaactctgtg agatggcggc gcgtctttga cttttccgcg
     4681 cggaacgtag ggccgaagca ataaaccttt ccgaacgccg ccgccgcggc ctcggcgtag
     4741 agctgtccgg tttgggccag gtaggccttc gagtcgaagt agtcgatctc gaaaaggccg
     4801 gaaactcctt ctccggcggc gccgctgatg atcgggctgt caatcagcaa aaagccgttt
     4861 tcctggaggt attcccttgc cgaacggata acacggtctc taatccgcat gatcgcctgc
     4921 tgacggctgc ttctgagcca gaggtgacga tggtcgagaa ggaaatcgat accgtggtcc
     4981 ttctttccga tggggtattc ctcagaagga ttctggaaaa cacgcagatc cgtcaccgaa
     5041 agttcaacgc cggaaggggc gcgttcatct tttcgaacag tgcccgtaac ctcaacggat
     5101 gcctccagcc acaacccctt agccgcggca aagagatctt cggaaacttc tttttttacc
     5161 atgactccct gtactgtgcc cgatccgtcg cgtagctgaa gaaagtgaat cttccctgaa
     5221 ctcctcaggt tgtaaagcca tccgcggata acgacagtct cctccacatg agaaggaaga
     5281 tctttgatgg aaacccataa tgctgtcatg cgtcatcaac ctccagacgt cttgaacgat
     5341 aagtgattat caaattccat gcggtatatg atacactata ctttaaaaaa aaggagagga
     5401 accagtgaat attccacgtc ttgtcctcgc ggaagaaaga agagagggta aagttgccca
     5461 gagtattctc gtcgcgtcag tcctgaaaag tctcggatac cctcttcgtt tgttttgcgc
     5521 aggagttgac gagcatcttg ttcgtttttt aaaacttgtc accgagcaag acgtaacggt
     5581 actcgatcct ttttcatgcg gaagcatgcg gaattttaaa ctcctcttcc aacgcgctgc
     5641 gtcgccggat accttgaaca tcatcttcgg ttcaatcggg gaacgtcggg atgaaaacac
     5701 ctttcctgta caccctctcc ccggcgagat tgcacgtatc cttgaatgcc cgctggtgac
     5761 gatgctctat gcggatagtt cagccgcagt cacctcaaga attctcgaaa atgttttcaa
     5821 ccagatatca gcacagggag tgattacttc cgcagtgatc ttctcctctg tcttgaatcc
     5881 tcgggaatat cagctgttgg agaccgaagc gggacgtcgc gttcctctac tttgcctggg
     5941 gtttattccg aaaacgcttg aaaaaaacaa cccgccgctc atggaactct gtcttcagga
     6001 tatggcccag cgagcggcgt tccctgtaaa agccgcgact gcgcaactgc tgggtatggt
     6061 cgaccaggtt gactggaatg tcctatgggg gatagcaaaa cagtgtaaag agtgcgtagc
     6121 tatggatgat cctttcaagg gaaaagccag cggcatg
//
LOCUS       DAOPKU010000128         6106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_13844_length_6106_cov_5.219468, whole genome shotgun sequence.
ACCESSION   DAOPKU010000128 DAOPKU010000000
VERSION     DAOPKU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13844_length_6106_cov_5.219468"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2595)
                     /locus_tag="PK849_05390"
     CDS             complement(97..2595)
                     /locus_tag="PK849_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPE65593.1"
                     /translation="MSKTSLPSQEPYSAVSGVNGTERRAWGTFVSTFLQTMAPVFFLI
                     VFLLVLLLYFFLPLLEESYLHQKRLMCKYLVQVQMTYLEALHTDVLKGTISMEEAQGR
                     ALRRLRMHRFGEAEKDYFWALGPERTLVMHPYRPDLEGINPDEAKGPDGELLRGLFDG
                     IEAAAARTPEGGHLDYLWHFKDDLGTLSAKTSHVARFVPWNWIVGTGVYLDDIHMEIA
                     SWRRRFTLLGLFAVLGSGGLSLFLSIRAVLLGRKAALFVVADRSLREKTRTLIQTEKQ
                     LAIITSVYDHSSEGVVITESDGTILRVNRAFTEITGFSRDEAIGRNPRILKSNTHDGE
                     FFERMWSDLLEKKRWSGEIWNRRKNGEAFPAMLNIVACSDDSGETTHYIGVFQDLSEL
                     HRSRDSLRHETFHDSLTGLPNRFLFSDRLAMALGRAKENAVLVSVIILGLDRFSSINK
                     TLGYPAGDLILKEAGSRIEAVVGETGTAARFGGDEFVILIPKSSDLHSVLRTTHRMLA
                     SLRAPFSIEGESLRLTASAGVALYPRDGDAPENLLQNASLAMERAKKEGGDSYRLFSE
                     TLDRKVQQRIRLEGDLRNAIDEEAFFIHYQPKISMIQGKTLSVEALLRWTNRENESIP
                     PDVFIPLAEDIGEINSLGRFVLERSCLQAKEWRSEGRDIVMCVNVSAKQLASERFVHS
                     VLGALERTGTPPHALELEITESAFMSNPEYATRVMEELKERGLRFTLDDFGTGYSSLS
                     YIRSLPLSGIKIDRSFTADLDNPKTRAIVSTIMHMAGELNLELTVEGIETTAELNTIR
                     SLAPRGGDILLQGYLFSRPIPPDTFSSLFDRDFM"
     gene            complement(2813..3553)
                     /locus_tag="PK849_05395"
     CDS             complement(2813..3553)
                     /locus_tag="PK849_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPE65594.1"
                     /translation="MIATPIAFLAGETELRREKPELVKAVIFDLDGTLIDSEENHYES
                     DRVLLARRGVTFSREDKKAFVGKDITEMVRRIKANYGLASDAQSLMDEKNAIYRSLAL
                     KSSRLYPPMKVVLEEFRKRKLPMAIATGSNSSIAGEILTSLGVRDFFTMIVSSSEVKK
                     GKPEPDIFLETARRMAIDPKEAIVFEDTRFGVEAALRAGMTCVALPAPEDDADHKLFQ
                     QADCLIKGGPDELDPQSFFWWFDTLAKL"
     gene            3996..6056
                     /locus_tag="PK849_05400"
     CDS             3996..6056
                     /locus_tag="PK849_05400"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE65595.1"
                     /translation="MKNRKGIFSSRRLSLMAKLGGGTALLVIVVVAITAGASLSRFSE
                     VLEENAHQKSILAAHGLESILEAKTAESRMATLFLVSAPGLADTVKAGNSKALAEQLT
                     PLWKEQTDLDFAAVLDPKGTVLLRLHEPDKNGDSLADEFNVRGAMKGQTTSAIEPGTQ
                     VPLSARTATPIKDASGALVGILTTGISFSREAFVDQAKELFDAEVTVFAGDTRLMTTI
                     RRDGERVVGTKLDPAIAAVVLSGKSYYGAAEILGAPYITAYEPILGPDGKPVGIYFSG
                     ISMAALATVQQSTFWVALGVAAVGLLFLGLLVRKIVKSIRTMVFFMKEVEAGRLYHIR
                     EEFGVTSHDEIGDMADALAEMVRVQREMISELKEKSVHLSALSEETAASTEEVTTTTN
                     EVAESNSKLAEQTRNGRLNAVESSKVMLEMSSLIQIAQTLASSADKNSGGMSEAVLEG
                     RETVFRTIERIEGVKGAVEETENVLSQLDAYSQRIGVVGDTITGIADQTNLLALNAAI
                     EAARAGEAGRGFAVVAEEVRKLAEQSQQGAREVSELVGKILDGTNLAVASMRKSREGV
                     EEGVSVAHVAGSALERIKEAIDKSIADIRRIIDTTNEEVARSDKVIKLIDTTASVMEN
                     TDDYVQNLAASMEETAAAMENVATSANEVSESSEELRRMTDRFQVEKDGGGTTSAIVP
                     KRGA"
BASE COUNT         1293 a         1687 c         1881 g         1245 t
ORIGIN      
        1 gaccctcctt ggccggagta tccggaccgg ttgtgaaagc agatacatca tagtcatttt
       61 tggatggagc gcaagggggg tgaaaagaga gactttttac atgaagtccc tgtcgaagag
      121 tgatgaaaaa gtatccggcg gaatcggacg agagaagaga tacccctgga gcagaatatc
      181 tcccccgcgc ggggccagcg aacggatggt attgagttcg gctgtcgttt cgattccttc
      241 gacggtgagc tccaggttga gttcgcccgc catgtgcatt atcgtcgaaa cgatggcgcg
      301 cgtctttgga ttgtcgaggt cggcggtgaa ggagcggtcg atcttgatgc ccgagagcgg
      361 gaggctgcgg atataggaga gcgaggagta cccggtgccg aagtcgtcga gggtgaagcg
      421 cagcccgcgc tccttgagct cctccattac acgcgtcgcg tactcggggt tcgacatgaa
      481 ggcggattcg gtgatttcca gttccagcgc atgcgggggc gtcccggtgc gttcaagcgc
      541 gcccaggacg gagtggacaa atcgctccga ggcaagctgt tttgccgaga cgttcacgca
      601 catgacgata tccctcccct cgctgcgcca ctccttcgcc tggaggcagg agcgttcaag
      661 gacaaatctt ccgagcgaat tgatctcgcc gatgtcctcc gcaagaggga taaaaacgtc
      721 gggtggaatg ctctcgttct ctctgttcgt ccaccggagc agagcctcca cgcttaaggt
      781 tttcccttgt atcatgctga tcttcggctg gtagtgaatg aagaaggcct cctcgtcgat
      841 cgcgttgcga agatctcctt cgagccgtat ccgctgctgc accttccggt cgagcgtctc
      901 ggagaagagg cggtacgagt cgcccccctc cttcttggcg cgctccatgg cgagcgaggc
      961 gttctgcagg aggttctccg gagcgtctcc gtccctcgga tagagcgcca cgcctgcgct
     1021 ggcggtgagg cggagactct ctccttcgat ggagaacgga gcgcgcagcg aggcaagcat
     1081 cctgtgtgtc gttcgaagca ccgagtggag gtcgctgctc ttcgggatga ggatgacgaa
     1141 ctcgtcgccg ccgaaccgtg ccgccgttcc cgtttcgcca acgactgcct cgatgcgcga
     1201 tcccgcctcc ttgaggatga ggtcgcccgc cgggtacccc agcgtcttgt tgatcgagct
     1261 gaacctgtcc agacccagta tgatcacgga gacgagaacg gcgttctcct ttgccctgcc
     1321 gagggccatc gcaagcctgt cggagaagag gaaacggttc ggcagtccgg tgaggctgtc
     1381 gtggaacgtc tcgtggcgaa gactgtccct gctgcggtgc agttcactga ggtcctggaa
     1441 gacgccgatg tagtgcgttg tctctccgga gtcgtcactg caggcgacga tgttcagcat
     1501 cgccgggaac gcctcgccgt tctttcgtct gttccaaatc tccccggacc accgtttttt
     1561 ctccagaagg tcgctccaca tacgctcgaa gaactctccg tcgtgcgtgt tcgacttgag
     1621 aatcctgggg tttcgtccta tggcttcgtc ccttgaaaaa ccggtgatct cggtgaacgc
     1681 ccggttgacg cgaagaatcg tgccgtcgga ttcggtgatg acgacccctt ccgacgaatg
     1741 atcgtagacg ctggtgataa tcgcaagctg tttttccgtc tgtatgagcg tgcgggtttt
     1801 ctcgcggagc gaacggtccg cgacgacgaa gagcgccgcc tttctcccca agaggaccgc
     1861 gcgaatgctc aaaaagagcg aaagaccgcc cgagccgagc accgcgaaga gtcccaacag
     1921 cgtgaagcgc cgccgccacg aggcgatctc catgtgtatg tcgtccaggt agacgcctgt
     1981 tccgacgatc cagttccacg gaacgaagcg ggcaacgtgc gaggtcttgg ccgagagcgt
     2041 ccccagatcg tccttgaagt gccacagata gtcgaggtgt cctccctccg gggttcgcgc
     2101 cgccgccgcc tcgatgccgt cgaagagacc gcgcagcagc tccccgtcag gccccttcgc
     2161 ctcgtcgggg tttatgcctt ccaggtcggg acgatacgga tgcatgacga gcgtccgttc
     2221 cgggcccagc gcccagaagt agtccttttc agcctctccg aagcgatgca tccgaaggcg
     2281 gcgaagggcc cgtccctgcg cttcctccat ggagatcgtt cccttgagga cgtcggtatg
     2341 gagcgcttcc aggtaggtca tctggacttg cacgaggtac ttgcacatca ggcgcttctg
     2401 gtgaaggtag ctttcttcga gcagaggaag gaaaaaataa aggagcagga cgagaaggaa
     2461 gacgatcagg aaaaagacgg gagccatggt ctgaagaaac gtcgagacga atgttcccca
     2521 tgcgcggcgc tctgtcccgt tgacgcccga aacagcggag tacggttctt gagaaggaag
     2581 cgacgtcttg gacatctcca ggaatctcct ttctcctgtt acataccgcc ctgagcatgg
     2641 aggttcacaa agattgtatc agatgcgttc ggctgcgtga gaatcttgag gctcatatct
     2701 gttccggaga gaggcgcaac ttgcagttca tgcaaaaaat ctgcggggcg acgcggggat
     2761 ctgccgatgg acggtacgcg tcgcctctgc ggatgggggt gcggagcgtg aatcagagtt
     2821 ttgcaagggt gtcgaaccac cagaagaacg attgcggatc cagctcgtcc ggcccgccct
     2881 tgatcagaca gtctgcctgt tggaagagct tgtggtcggc gtcgtcttcc ggggcgggga
     2941 gggccacgca ggtcatgccc gcgcgaaggg cggcttcgac gccgaaccgg gtatcctcga
     3001 agacgatggc ttccttagga tcaatggcca tccggcgcgc cgtttcaagg aaaatgtcgg
     3061 gttcgggttt ccccttcttc acttcggagg aggagacgat catggtgaaa aagtcgcgca
     3121 cgccgagcga cgtcagaatc tccccggcga tggaggagtt ggaaccggtc gctatcgcca
     3181 tggggagctt gcgcttgcgg aactcctcga ggacgacttt catggggggg tacaggcggc
     3241 tggatttcag cgcaagcgag cggtagatgg cgttcttctc gtccatcagg ctctgcgcgt
     3301 cgctcgcaag gccgtagttg gccttgatcc gccggaccat ctctgtgatg tccttcccga
     3361 cgaaggcctt cttgtcctcc cgtgaaaaag tgacgccccg gcgcgcaagg agcacccggt
     3421 cgctctcgta atggttctcc tcgctgtcga tcagagtgcc gtcgagatcg aagataaccg
     3481 ccttaacaag ctccggtttt tcccggcgca gttccgtttc gcccgcgaga aatgcaattg
     3541 gtgtcgcgat cattgtgatt ctcccttccc gaatgctctg gtccggaaga attgtaacat
     3601 ggaatttctt tcaggaaaat acgatctcca cctgaattca actctgcatg ttgttgcgcc
     3661 tccgtccgcc gcagtgcggc ggaaaaaccg aaaaatagta tgcaaacgaa gatgcaattg
     3721 aaatagtcga tagcaagagc cgataaagag aatggaagga tattcctttt ctttaaggtt
     3781 tcttcgagat gagaagccaa gttgtaatat tttgttactt tcaagtgtgc gcttttctga
     3841 gttcgttata aatttattta taaatggaac tatagaaaat taacaaacac attaaagctc
     3901 atttatataa tccgctgtaa tgaattgtct ttcgagaaga agatgttaca atgtataaac
     3961 tggcttttaa attttcattt cacggagggg aaaaagtgaa gaatagaaaa ggaatcttct
     4021 cgtcgcggcg tctctctctt atggcgaagc tgggcggagg aacggccctg ctggtcattg
     4081 ttgttgtggc cattactgcg ggcgcctcgc tcagccgttt ttcggaggtc ctggaggaga
     4141 acgcgcacca gaagtccatt ctcgccgcgc acggactgga gagtattctc gaggcgaaaa
     4201 ctgcggaatc aaggatggcg acgctctttc ttgtctccgc cccggggctt gccgacacgg
     4261 tgaaggcggg gaattccaag gctctcgccg aacagctgac gcccttgtgg aaagagcaga
     4321 ccgatcttga cttcgcggcg gtgctcgatc cgaagggcac ggtgctcctc cgtcttcacg
     4381 aacccgacaa aaacggcgat tcgctggccg atgagttcaa cgttaggggc gccatgaaag
     4441 gacaaacgac cagcgccata gaacccggaa cgcaagtacc cctctcggcg cgaaccgcga
     4501 ccccgatcaa ggacgcctcc ggagcgctcg tgggcattct caccacaggc atttcatttt
     4561 caagagaggc gttcgtcgac caggcgaagg agctcttcga tgcggaggtt accgtctttg
     4621 ccggggatac ccgtctgatg accaccattc gccgcgacgg ggagcgtgtt gtcgggacga
     4681 agctcgaccc ggcgatagca gccgttgtgc tctcggggaa gagttattac ggcgcggctg
     4741 aaatactcgg cgccccctac atcactgcgt acgaacctat ccttggaccc gacggcaagc
     4801 ctgtgggcat ttacttctcc ggtatttcca tggcggcgct ggccactgtg cagcagtcga
     4861 ctttctgggt agcgctgggg gtggcggcag tcggtttgct cttccttggg ttgctggtcc
     4921 ggaagatcgt aaagagcatc cggacaatgg tcttcttcat gaaggaggtc gaagcggggc
     4981 gcctctacca tatcagagag gagttcggcg tgacctccca cgacgagatt ggtgacatgg
     5041 ccgacgctct tgccgaaatg gtccgcgtgc agcgggagat gatcagcgaa ctgaaggaga
     5101 agtccgtcca tctttcagcc ctttcggagg agaccgctgc ctccaccgag gaagtgacca
     5161 ccacgaccaa cgaagttgcc gagagcaatt ccaagctggc cgagcagacg aggaacggac
     5221 ggctcaacgc cgttgaatcc tcgaaagtca tgctggagat gagcagcctt atccagatag
     5281 cgcagaccct tgcgtccagc gcggacaaga actccggagg aatgtcggaa gctgtgctcg
     5341 aaggccggga gacggtgttc agaaccatcg aacgcatcga gggagtcaag ggggcggttg
     5401 aagagaccga aaacgtgctc tcgcagctcg acgcctactc gcagcggatc ggcgtggtcg
     5461 gcgacaccat caccggcatc gccgaccaga cgaacctgct ggcgctcaac gccgcgatag
     5521 aggctgcgcg ggcaggggaa gccgggcgcg gattcgccgt cgtcgccgaa gaagtacgca
     5581 aactcgccga acagtcgcag cagggcgctc gcgaggtgtc ggaactcgtc ggaaagatcc
     5641 tcgacggtac gaacctggcg gtagcgtcga tgcgcaagag tcgcgaggga gtcgaggagg
     5701 gcgtttccgt cgcgcacgtt gcggggagcg ctcttgagcg catcaaggag gccatcgata
     5761 aatcgatagc ggatatccgc cggattatcg acaccaccaa cgaagaggtc gctcgatcgg
     5821 acaaggttat caagctcatc gacacgaccg cgagcgtgat ggagaacacc gacgactacg
     5881 tccagaacct cgcggcctcg atggaggaga cggccgccgc gatggagaac gtcgcgacga
     5941 gcgcgaacga agtcagcgaa tcctccgagg agctcagacg gatgaccgac cgtttccagg
     6001 tagaaaaaga cggcggggga acaacgtccg cgatcgtccc gaagagagga gcctgagcgg
     6061 cctccggaga gtcccgtcct gagaaagaac gagatgccgg ggtgtt
//
LOCUS       DAOPKU010000129         6100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_13866_length_6100_cov_4.759801, whole genome shotgun sequence.
ACCESSION   DAOPKU010000129 DAOPKU010000000
VERSION     DAOPKU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13866_length_6100_cov_4.759801"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..955)
                     /locus_tag="PK849_05405"
     CDS             complement(149..955)
                     /locus_tag="PK849_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HPE65596.1"
                     /translation="MNIMDILGGLMQAKTAPSATRRMQNVFGADGASLPSDDQKSGLG
                     LEDLLGGLLGAGKTSASDGGGMLGQILNQAASAVGGKKNLAVGGLSVLMGSLIGGGKS
                     AATGALGGGLMALLASMAMNAMQNSGKNTGQLPVGLKAPETEEEQHDLENGAELVVAA
                     MINAAKADGRIDKDEYRLITGKLQKAGLQQDDMYYIMEELQKPMNTEGLVRAAKNNPG
                     LAAQIYAATLMAIEVDTPAEKTYVEELASGLGLSNDMVRTIEQMAGMQQI"
     gene            1545..1865
                     /locus_tag="PK849_05410"
     CDS             1545..1865
                     /locus_tag="PK849_05410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPE65597.1"
                     /translation="MGVDIHVPHGAPIRAAKAGAVVFSGLRGGYGNAVIIQHDAKSSS
                     LYAHCSRLHVKRGQKVHQGQVIAAVGETGRATGPHLHFEIRTGGGAVNPLEFWRKHAE
                     SSAQ"
     gene            complement(1922..3244)
                     /locus_tag="PK849_05415"
     CDS             complement(1922..3244)
                     /locus_tag="PK849_05415"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HPE65598.1"
                     /translation="MRKTFLLFVCLLLFNPFPAVAEQTFREFSGEFTPQNNGERLLAF
                     LVSVTDPESLELQMDALPGDDGAIRAVSFALHGGALGGFRIERLTLESAFIRLNPPSE
                     WIRGNRHSLRVCNALRSNFELVIDEHDILEALKKYASGSWSRISVDLTSGELRIQGRY
                     KPGATGFSLMAGITTRLELRDGKRVMLKAPEIRINGEDKTSLFQRDLERLRPVLDFNG
                     FPPALTLSRLSVDKGKLLLATATRPKAVGGIRYRYVRDRERPFSIHPPESFDFAERQF
                     RNGDIVLVGGKTWRSKIVNLFEDGNAAFSHSGIVRIIGGVPYIIHASPESELVRMERA
                     ETFLSPKKVDRASIYRLRGNPGAAETAGRKALEYYEERVPFDTAFDGEDHERLYCTEL
                     IWRAYTHAGIDLADGLWYSLYNPVLSGRLLLPNSLTRSPLLEETAILR"
     gene            3535..4083
                     /locus_tag="PK849_05420"
     CDS             3535..4083
                     /locus_tag="PK849_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPE65599.1"
                     /translation="MRILFVDGNPSAEDAVFGERLAAMEHGVAGHDVTRFCLRGKAIH
                     SCIGCFACWMRTPGTCIFQDDHVELLRLFLKSDVAVFASPLIAGFYSALLKTAIDRVI
                     PLVLPYIEFADGECRHPLRYGRAMPKMGFLYEPEEDTDEEDLRIVSTAFERFARNGHT
                     RFLFSRPLSEDPEEVRHAIEHL"
     gene            4064..4741
                     /locus_tag="PK849_05425"
     CDS             4064..4741
                     /locus_tag="PK849_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPE65600.1"
                     /translation="MRLNIFNCSPRGKRSNTKVLTNAFAEGFLRGAAGAPELNEVREF
                     FISEPDDFRRAAEAFGEPSAALISFPVYVDAMPAIAKSFIEEIAAFKGRCSSTRMLFL
                     IQTGFPEETHTRPMKAYLEKLAKRLGAPCDGVIRMGGGEGARDRVRGSKGGGKMFERY
                     RMLGRGYAANGVLDEAILAKLAGPKRLPALLAPLVLPLANYFFWDRELKRNGAYEKRR
                     DRPLERE"
     gene            complement(4860..5192)
                     /locus_tag="PK849_05430"
     CDS             complement(4860..5192)
                     /locus_tag="PK849_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE65601.1"
                     /translation="MKPYDMAGIDPFTGRPGFIGKFVHSDQMTLAHWEIAGGTELTEH
                     SHPHEQITLLLAGKMELRSGDHVAILTPGQGIVFAGGETHGGYAHEDCVVVDIFCPVR
                     EDFKSQQK"
     gene            5621..>6100
                     /locus_tag="PK849_05435"
     CDS             5621..>6100
                     /locus_tag="PK849_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPE65602.1"
                     /translation="MKKGILFSYGKYLPLTEKTPMLSLCEGDTPLVPLRNLSRELGVG
                     LFGKYDGLNPTGSFKDRGMVLAVSKALEEGAKAVICASTGNTSASAAAYAAVAGIPCF
                     VLLPAGKVALGKLAQAMIYGARVLAVRGNFDRALEMAREGAEQDGFAIVNSVNPYRLW
                     "
BASE COUNT         1151 a         1671 c         1879 g         1399 t
ORIGIN      
        1 caagaagatg taggttaagc ccctctctgc ggagaggggc tttttattcc tctgcagagg
       61 caacccaggc cttcgctcct tacggcatgt tttccaacag cattcgcagc tatttcgccg
      121 ttttgtcgct gcgaaggcca tgcgtcgttt atatctgctg cattcccgcc atctgttcta
      181 tagtgcgcac catgtcattg gagagtccca agccggatgc cagttcttcg acatacgttt
      241 tttccgcggg ggtgtccacc tcgatggcca tgagagtagc ggcgtagatc tgcgccgcga
      301 gcccggggtt gttctttgcc gcccggacga gcccttcggt gttcatgggt ttttggagtt
      361 cctccatgat atagtacatg tcgtcctgct ggaggcccgc tttctggagt tttcccgtaa
      421 tcagcctgta ctcatctttg tcgatccggc cgtctgcctt ggctgcgttg atcattgccg
      481 ccacaaccaa ttccgctccg ttctcaaggt cgtgctgttc ctcttcggtc tccggggcct
      541 tgagtcctac gggaagctgt cctgtgtttt tcccgctgtt ttgcatcgcg ttcattgcca
      601 tcgaggcgag cagagccatg agcccgccgc cgagagcgcc ggtagccgcg ctcttgcctc
      661 cgccgatgag ggagcccatg agtacgctca atccgccgac agcaagattt ttctttccgc
      721 cgacagcgct tgcagcctgg tttaaaatct gcccgagcat accgcctcca tcggaggcgc
      781 tcgtcttccc ggcgcctaaa aggcctccca acagatcctc aaggccgaga ccgctcttct
      841 gatcgtcgga gggaagggat gcgccatcgg cgccaaaaac attctgcatc ctcctggttg
      901 ccgatggagc cgtctttgcc tgcatcaagc cgcctagaat atccattatg ttcattcttc
      961 atcctcctct gtatgctttc ttctttttgg tttcggtcga gaggctcttc cgttgtagtt
     1021 ctcagagtat gcatcctcat tatctcactt gagcattgtt ttgcctatca atatgcttct
     1081 cttacatatc ctcttcttct cttgaccgaa ggatgaatgg caaaatacaa agtgtctgcc
     1141 gttcgtttgt ctctttgaca ggaagccccc ttgtttgctc tgcggctttg aggcgcgtgg
     1201 acgatgggat tttcgggaaa agactgtaca atatgataaa gagatccgcc acttttttgg
     1261 ggcggcgcgt ctcaagagaa tcgagagggt gttttgtgat gtatcggggt gaaaacgttt
     1321 ggttgtatcg actcataatg atcgcagcgg tgttgggctg cctgtgcgta ccttgtttta
     1381 ccgtgttttc tcaaccgcag gaaaatgcgc cgcactcctc cggggtctcc atttcccagg
     1441 ggattgcgaa gctggggttt ttatggccct tgttcgtgcc gggaaaggtg acaagccgct
     1501 tcggccagag gacgcacccg attaccaagg agccggacta tcacatgggg gttgatattc
     1561 atgttcccca cggagcccct attcgggcgg caaaggcggg cgccgtggtt ttctccggtc
     1621 tgcgcggagg gtacggcaac gcggtcatca tacagcatga tgcgaaaagt tcctccttat
     1681 atgctcactg ctcccgtctt cacgtcaagc gagggcagaa ggtgcatcaa ggacaggtaa
     1741 tcgcggcggt cggcgagacg ggaagagcga cggggccgca ccttcatttc gagatacgca
     1801 cgggcggcgg cgcggtcaat cctttggagt tctggaggaa gcacgcggaa tcgtcggcgc
     1861 agtgaaaacc gcagtgcggc cgttcacatc ggacatcgaa gcgccgcgcc gccctcccgg
     1921 tttaccgcag gatcgccgtt tcttcgagca gcgggcttcg ggtcaggctg ttcggaagca
     1981 gcagtctgcc ggagagcaca gggttgtaca gggagtacca cagtccgtcg gccaggtcga
     2041 tacccgcgtg cgtgtacgct ctccagataa gctcggtgca gtaaagcctt tcgtggtctt
     2101 cgccgtcgaa cgcggtgtcg aacggcacgc gctcttcgta gtactcaagg gctttcctgc
     2161 cggctgtttc agcggcgccc ggattccccc gcagacggta gatacttgcc ctgtccactt
     2221 ttttcgggga taaaaaagtc tccgcgcgtt ccattcgcac cagctcggac tcgggagatg
     2281 catggatgat gtacggaacg ccgccgatta ttctgacgat gccgctatgt gaaaaggcgg
     2341 cgtttccgtc ttcaaaaaga ttgacgatct tgctccgcca ggttttcccg ccgacgagta
     2401 cgatatctcc gttccggaac tgccgctcgg cgaagtcgaa cgactccggc gggtgaatgg
     2461 agaaaggtct ttcccggtcg cggacgtagc ggtagcgtat gccgccgacc gccttgggtc
     2521 gtgtcgcggt cgccagaaga agcttccctt tgtcgacgga gaggcgtgag agcgtcagcg
     2581 ccggaggaaa accgttgaag tcgagaacgg ggcggagccg ttcgagatct ctttggaaaa
     2641 gagatgtctt gtcctcgccg ttgatgcgta tctccggagc tttcagcatg acgcgctttc
     2701 cgtcgcgcag ttcgagcctc gtcgttattc cggccatgag ggagaagcct gtcgcgccgg
     2761 gcttgtagcg cccctgtatg cgaagttcgc ccgacgtcag gtccacggag atgcggctcc
     2821 agctccccga ggcgtatttc ttcagggctt cgaggatatc atgttcgtcg atcacaagtt
     2881 cgaagttgct ccggagcgcg ttgcataccc ggagcgaatg ccggttgccg cgaatccact
     2941 cagagggcgg attgagccgt ataaatgccg actctagggt cagccgttct atgcggaacc
     3001 ctccgagagc gccgccgtga agagcaaaag aaacggcccg gatggcgccg tcgtctcccg
     3061 gaagcgcatc catttgtagc tccagcgact ccgggtcggt gaccgagacc agaaacgcga
     3121 gcagccgctc tccgttgttt tggggggtga actcgccgga aaactcgcgg aaggtctgct
     3181 ccgcgacggc tgggaacgga ttgaagagca aaagacatac gaagagaagg aatgttttcc
     3241 tcacgctgat cccccctttc gatgcagtga aaaagcggac ggcttcgcgt atccgtgatt
     3301 tttctcggta gcaacgccca tggtgtggtg tgtatcgccc aaaataaaag aattctgtag
     3361 tattgtatca gagaactgta ttcgaggcga agcgcctgta ttataatgac gctttatttg
     3421 cacctgagaa aagaatgcaa tcaaagatgg aagtggacgt tcagcccggg gcgctctgga
     3481 atatttttca gccaatcgtt ccggggattt gtaaggatgg cgaggtgttg cgtattgaga
     3541 atcttgtttg tcgacgggaa tccaagcgcg gaagacgccg ttttcggcga gcgtcttgcg
     3601 gcgatggagc acggtgttgc ggggcacgat gtcacgaggt tctgtcttcg gggaaaggcg
     3661 attcactcct gcatcggttg tttcgcgtgt tggatgcgca ccccggggac atgtatcttc
     3721 caggatgatc acgtcgagct gctgcgtttg tttctgaaat cggatgttgc cgtctttgct
     3781 tctcctctga tcgcgggttt ttactccgcg ttgctgaaga cggcgatcga ccgcgtcatt
     3841 ccgcttgttc tgccgtacat cgagtttgcg gacggggagt gccgtcaccc gctgcggtac
     3901 ggccgtgcca tgccgaagat gggctttctc tatgaaccgg aagaggatac ggacgaggag
     3961 gatctccgga ttgtctccac cgcgttcgag cgtttcgccc gcaacgggca cacgcgtttc
     4021 ctgttctccc ggcctttgag tgaggatccc gaggaggtgc gccatgcgat tgaacatctt
     4081 taactgctcg ccgcggggca agaggagcaa caccaaggtg ttgacaaacg cctttgcgga
     4141 gggctttctc cggggagcgg cgggtgcgcc ggaactgaac gaggtcaggg agtttttcat
     4201 atcggaaccg gatgacttcc ggcgggcggc ggaagctttc ggagagccgt cggctgccct
     4261 gatctccttt ccggtctatg tggacgcgat gcccgctatt gcgaaatcct tcattgaaga
     4321 gatcgccgcc ttcaaggggc gctgctcgtc gacgagaatg ctctttctca tacagacggg
     4381 attcccggaa gaaacgcata cgcggccgat gaaggcctac cttgaaaagc tggcgaaacg
     4441 cctcggcgcc ccttgcgacg gagtcatccg gatgggcggc ggcgaggggg cgcgggaccg
     4501 tgtgcgcgga tcgaagggcg gcggaaaaat gttcgaacgc taccggatgc tcggcagagg
     4561 gtatgcggcg aacggcgttc tcgacgaggc gatccttgcg aagctcgcag ggccgaaacg
     4621 ccttcctgcg ctgttggcgc cgctcgtttt gccgctggcg aactattttt tctgggacag
     4681 ggagctgaag cggaacggcg cgtatgaaaa acgccgtgat cgaccgttgg aacgggagta
     4741 aaaacggcgg aaactgtgcg ttgtacggaa aaatgctccg gcgggatgga atgacgccgg
     4801 atcgcgcgca cgcggaagaa acggcgggag gagctgttcg ctctctcccg ccgtgccgtt
     4861 tacttctgtt gactcttgaa gtcttcgcgc acggggcaga agatatccac gacgacgcag
     4921 tcttcatgag cgtagccgcc gtgcgtttcc cctcccgcga agacgatccc ctgtccgggg
     4981 gtgagaatcg ccacgtggtc gccggagcga agctccatct ttccggcgag cagcagcgta
     5041 atctgctcgt gggggtgact gtgctcggtg agttcggtgc ctcccgcgat ctcccagtgc
     5101 gcaagcgtca tctggtccga atggacgaac ttgccgatga atccgggcct tcctgtgaac
     5161 ggatcgatac ctgccatgtc gtagggtttc atctcgtatg ccacgctcct ttatgaatat
     5221 ctgtcctccg cgccccctct tttccgggtc ccctcttgac actttccggc gacgaaggta
     5281 taagaggaaa atgttttcca atgatatgat aaaaagacga tgaagggaag agtaccttct
     5341 ctccgaagga cagagaggaa gggccgaggc tggaagccct tctcccgagg agcgcagaga
     5401 agaccacccc tgagtcggcg tcgaagttcg cgcggtttcg cacgcggagg gaagacgccg
     5461 gggtccgccc cttacagcgg tgtcgagttg ccggtatcct cccggcggat gcgcgaagca
     5521 gccgagagag gtccggaaat ggagtggaac cgcgatgagc ctgcatcgtc tcctgtattg
     5581 tggagggtgc gggctttttg tattacagga gggatgttcc atgaagaagg gcatactgtt
     5641 ttcttacgga aagtatcttc cgctgacgga gaagacgccg atgctgtcgc tgtgcgaagg
     5701 cgacacgccg ctcgttccgc tgcggaattt gagccgggag ctcggcgtcg ggctgttcgg
     5761 gaagtatgac ggcctgaacc ccaccggttc gttcaaggac agggggatgg tgctcgcagt
     5821 gtcgaaggcg ctcgaggaag gggcgaaggc ggttatctgc gcctccaccg gcaatacgtc
     5881 ggcctctgcc gcggcctacg cggccgtcgc gggcattccc tgcttcgtgc tccttcccgc
     5941 gggcaaggtt gcgctgggga agctggcgca ggcgatgatc tacggagcca gggtgctcgc
     6001 cgtcagggga aatttcgaca gggcgctcga gatggcgcgg gagggagcgg agcaggacgg
     6061 gttcgccatc gtgaactccg taaatccgta ccgtctgtgg
//
LOCUS       DAOPKU010000130         6089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_13909_length_6089_cov_4.418131, whole genome shotgun sequence.
ACCESSION   DAOPKU010000130 DAOPKU010000000
VERSION     DAOPKU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13909_length_6089_cov_4.418131"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK849_05440"
     CDS             complement(<1..568)
                     /locus_tag="PK849_05440"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPE65603.1"
                     /translation="MTVRYPRLLVSRNRIFENAARVSERCARAGIALTGVTKGVCARE
                     ALVRAMAEGGCDELGDSRIDNLAAIRTMNVGLPLLLLRIPMPSEIAATVRIADRSLVS
                     MAQTISLIDRECRAAGRTHEIILMTDLGDLREGVWMGRVDEIAPVLKASPRVRCVGVG
                     VNFGCFGGVLPVPEKLEQLLSVGKELERF"
     gene            complement(571..1413)
                     /locus_tag="PK849_05445"
     CDS             complement(571..1413)
                     /locus_tag="PK849_05445"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPE65604.1"
                     /translation="MAIIGAHVSIAGGLEKSIERGEELGCEAIQIFTKNQLQWRSAPL
                     SQSRGERFYRSWAASSIEEIVVHASYLINLAATDATGAKSIAALEDEIERCDLLGIDN
                     LVLHPGSSRGRDVESALDLVAERIASILDRTAGKRVRILLETMAGQGDLLGRNFKQFR
                     RILDALDWPSRVGICMDTCHMFAAGFDMRTEAAYQRLLDQVDDTVGLDRVGCWHFNDS
                     IHDMGRGLDRHAHIGEGTLGYAPFSFVLNDPLWEHTPCILETPQNASGHAGNMSILRK
                     LRGG"
     gene            complement(1430..2071)
                     /locus_tag="PK849_05450"
     CDS             complement(1430..2071)
                     /locus_tag="PK849_05450"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPE65605.1"
                     /translation="MKERGGERCFSPERSGKGLLMNHPVGVSFPSWEELPSGDMRQSA
                     VLVPLFPSGDELRILFLRRSPHLSRHGGEICFPGGMKDADDLSPLHTALRETEEETGI
                     SREAVEPLLLLPPEYTVVSNVEVFPVLGIVSGVDPERGLALSPSEIAASYSVDIALFP
                     AEPRRKEILFPSFTKGMRESREHTVSYPEYDLPDGSVVWGVTARILEKVCRLL"
     gene            complement(2068..3819)
                     /gene="pyk"
                     /locus_tag="PK849_05455"
     CDS             complement(2068..3819)
                     /gene="pyk"
                     /locus_tag="PK849_05455"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPE65606.1"
                     /translation="MTKVKIVCTIGPACSNYETLSAMAKAGMNVARLNFSHGAYDGHQ
                     KMLELVRRVEKDLNTPIAALLDTKGPEIRTGKVEGGTVALEQESLLVLTTDEIVGTSS
                     RVFMNYPQLAREVAVGQEIFIDDGTLHLKVEEIRGGDVACRVIVGGLLADTKGINIPG
                     AELSLPALSEKDIEDIKWGVQHEMEYIAVSFVKNRRDIMEVRRIMEEFGEPMKVIAKI
                     ETRQAVQNIEEIVEVVDGMMIARGDLGVEIPTEDVPLQQKRIIDICRAKGKAVIVATQ
                     MLDSMIRNPRPTRAEASDVANAVLDGADAVMLSGETAKGAYPLRSVETMKRIVARAES
                     EIELWERPLHAVSTTMGVPDAVSSAAVLIARQMKASAIVSMTKSGSTAQMVSKHRPPC
                     TILGATPSQRTWRELSLYWGVLPLMKEEMTDQNTAVDSVLGSCVASGHIREGDLVVVT
                     AGVPLGTAGTTNLVQVHIAGKILLKGLSLLKREARGAVCVARSAAEAREKMTKGAVLV
                     ARNTDKEYLPAMRLASAVVVEEGGLTSHTAIVALELGIPCIVSAEGALSLLSDGMLVT
                     VDGYRGILYHGRVKLTA"
     gene            complement(3867..>6089)
                     /gene="dnaE"
                     /locus_tag="PK849_05460"
     CDS             complement(3867..>6089)
                     /gene="dnaE"
                     /locus_tag="PK849_05460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPE65607.1"
                     /translation="DTDVSDKGRDELLHYITEKYGHDKVSQIVTFGRLKSRAAVTDVG
                     RVLGMPVRDVVAITRLIPPMGIHSIGEAVEQVPELAELKKSNPMVARVLDVASNIEGL
                     ARHCSQHAAGVVITPVPITDLVPVRRIGQDQIVTQYPMEPIEKLGLVKMDFLGLRTLS
                     VIEEALQNISLGGKSLPDMERIPLDDRKTYEMLQKADTLGVFQLESTGMRQLLKKLQV
                     DCFEDLIAVLAMYRPGPLGSGMVDQYIECKHGRATVKYLHPLLEGVLRETYGVVLYQE
                     QVMQCAAILAGYSLGEADLLRRAMGKKKADVMEQQRAKFVEGCTQRNINEKSAQEIFD
                     IIQEFAGYGFNKSHSAAYALITYQTAWLKANYRSEFMAAYLSSQIGSKKEDLAAYVRE
                     VRNSGIDVLPPDVNSSMASFTPVGEVVRFGLGAVAKAGHTAVEAILAARNTGTPFLSM
                     WDFLCRVDLRVVNKAVVENLVRAGAFDNLENNRRKLLEGLPELISAAQKKCANSNQCS
                     LFESLPEEDAAGPDLPDVSDFTLLERLEMEKDAVGLYISGHPFEQYEKKVRMLSNCTI
                     AEVRSWRSKRIPPLVGGLVLSVKERTTKNGDPMGIVEIEDAESRIEAVCFPRTWEPLR
                     GTIQPGRVCLVSGFPEERGDTNIIAKSITLLDEDDGRNGTTSFVRISVLADFLQNVSF
                     KDFIRALKACPGKSPVLLELRNDSESTVLYLQDIKVDGRGGIAPLLSEVIPEELFQIG
                     "
BASE COUNT         1139 a         1930 c         1787 g         1233 t
ORIGIN      
        1 gaaagcgttc cagctctttt cctacggaga gcagctgctc cagcttctca ggcacgggga
       61 gcaccccccc gaaacagccg aagttcactc cgacgccgac acagcgaaca cgcggagatg
      121 ctttcagcac cggggcgatt tcgtcgacgc gccccatcca cactccctcg cggaggtccc
      181 cgaggtcggt catgaggata atctcgtgcg ttcgtcccgc agcgcggcac tcccggtcga
      241 tcagggagat cgtctgcgcc atcgagacaa gactcctgtc cgcgatccgg acagttgcgg
      301 cgatttcgct gggcatggga atccggagaa ggagcagcgg aagtccgacg ttcattgtac
      361 ggatggcggc cagattgtca atcctgctgt cgccgagttc gtcgcagccg ccctccgcca
      421 tcgcccgcac gagagcctcg cgcgcgcata cgccctttgt cacgccggtg agggcgatcc
      481 ccgcgcgagc gcatcgttca gagaccctcg ctgcgttttc aaagatccga ttcctggaga
      541 cgagaagccg cggatagcgg actgtcattc ttatcctcct ctcagtttac gcagaataga
      601 catatttccg gcatggccgg aggcgttctg gggcgtttcg agaatacagg gcgtgtgttc
      661 ccagagagga tcgttgagga cgaacgaaaa cggagcgtat ccgagagtgc cttctccgat
      721 gtgcgcgtgc ctgtcgagcc ctctccccat atcatggatg ctgtcgttaa aatgccagca
      781 cccgaccctg tcgagcccca cggtatcgtc cacctgatcg agcaggcgct ggtacgccgc
      841 ttcggtgcgc atgtcgaatc cggccgcgaa catgtggcag gtatccatgc aaatacccac
      901 gcgcgacggc cagtccagcg cgtcgagaat tctcctgaac tgcttgaaat tcctgccgag
      961 gagatctccc tgacctgcca tggtctcaag aagaatacga acgcgtttcc ccgccgtcct
     1021 gtcaagaata gaagcgattc gctccgccac caggtcaagg gctgattcta cgtctcttcc
     1081 gcgcgacgac ccggggtgaa ggacgaggtt gtcgatcccc agcaggtcgc agcgctctat
     1141 ttcatcctcc agtgcggcga tgctcttcgc tccggtggcg tccgtcgccg ccaggttgat
     1201 gaggtacgaa gcgtggacga cgatctcttc gatagaactc gcggcccagg agcggtaaaa
     1261 cctctcgccc ctggattgcg agagaggggc gctccgccat tgaagctggt tcttcgtgaa
     1321 aatctgtata gcttcacatc ccagctcctc gccgcgctcg atgctctttt ctagccctcc
     1381 ggcgatggag acatgggcgc ctattatcgc cattgatctt ctcccttctt cacagcaggc
     1441 gacacacctt ttccaatatc ctcgcggtaa ccccccaaac gaccgagccg tcgggaagat
     1501 cgtactccgg ataggaaacc gtgtgttctc tgctttctct catccccttc gtgaacgaag
     1561 gaaaaagaat ttcttttctc cggggctccg ccggaaaaag ggcgatgtcc accgaatacg
     1621 aggcggcaat ctccgaggga gagagcgcaa gcccccgctc cggatcgacg ccggagacaa
     1681 tgccgagcac cggaaagact tccacattgc tgaccacggt gtactcgggg ggcagcagaa
     1741 gaagcggctc gaccgcctcc cgggaaatgc cggtctcctc ctcggtttcg cggagcgcag
     1801 tgtgcaacgg cgataggtcg tccgcgtcct tcatcccgcc gggaaagcag atctctcccc
     1861 cgtggcgtga aagatggggc gagcgacgca aaaaaagaat gcgaagctca tccccggaag
     1921 ggaagagcgg aacgaggacg gcgctctgcc gcatgtcccc ggacggaagc tcctcccacg
     1981 aagggaacga aacgccgacg gggtgattca tcaacaatcc tttcccggaa cgttccgggg
     2041 agaagcatcg ctcgccgccc cgctccttca tgccgtcagc ttcacccggc cgtggtagag
     2101 aatacccctg tacccgtcga cagtgacgag cattccgtcg gagagaaggg agagggctcc
     2161 ctccgcgctg acgatgcacg gaattccaag ctccagcgcc acgatcgccg tgtgggaggt
     2221 aagccccccc tcttcgacga caacagcgga ggcgagccgc atggccggaa gatactcttt
     2281 gtcggtattc ctggcgacga gcacggcgcc tttggtcatc ttctccctgg cctcggcggc
     2341 gctccgagcc acacagacgg cgcccctcgc ctctctcttg agcagcgaga gtcctttcag
     2401 cagtatcttg cccgcaatat gcacctggac aaggttcgtg gtccccgccg tgcccagcgg
     2461 aacgccggcg gtgacgacca cgaggtctcc ctccctgatg tgcccgcttg ccacgcagga
     2521 gccgagcacg gagtccaccg cagtgttctg gtcggtcatc tcctctttca tcagaggaag
     2581 aactccccag tacagcgaaa gctcccgcca tgtacgctgg gaaggcgtcg cgccgaggat
     2641 tgtgcacgga gggcggtgct tgctgaccat ctgcgcggtg ctgccgctct tcgtcatcga
     2701 gacgatggcg gaggccttca tctgccgcgc aatgagcact gcggcgctgc tcaccgcgtc
     2761 cggcacgccc atcgttgtgg aaacggcgtg cagcgggcgc tcccaaagct cgatttcact
     2821 ctcggctctg gcgacgatac gcttcatcgt ttccactgag cggagaggat acgctccttt
     2881 tgccgtctcg ccggagagca tgacggcgtc ggctccgtca agaaccgcat tcgccacgtc
     2941 gctggcctcg gccctggtag gccgggggtt gcgtatcatc gagtcgagca tctgcgtcgc
     3001 gacaataacc gccttcccct tggcgcggca gatatcgatg atccgcttct gctgcaaagg
     3061 cacgtcttcc gtgggaattt ccaccccgag atcgccgcgg gcgatcatca ttccgtccac
     3121 gacctcgacg atctcctcga tattctgcac cgcctgacgg gtctctatct tggcgatgac
     3181 cttcataggc tcgccgaact cctccatgat gcgccggact tccatgatat cccggcggtt
     3241 tttgacgaag gagacggcga tatactccat ctcgtgctgt actccccact tgatgtcctc
     3301 gatatccttc tcggagagcg cgggaagaga gagttccgct cccggaatat tgattccctt
     3361 ggtatcggcg agcagtcctc ccacaatgac ccggcaggcg acgtcgccgc cgcgtatctc
     3421 ctcgaccttt aggtgaagcg tgccgtcgtc gatgaagatc tcctgaccga ccgctacctc
     3481 gcgcgcaagc tgcgggtagt tcataaagac ccgcgagctg gttccgacga tctcatcggt
     3541 cgtcaggacg aggagactct cctgttccag ggcaacggtt cccccctcaa ccttgccggt
     3601 acggatctcc ggtcccttgg tatccagcag cgccgcgatg ggggtgttca gatccttctc
     3661 gacccggcgg acgagctcca gcattttttg atgtccatcg taggcgccgt ggctgaagtt
     3721 caggcgcgcg acattcatgc cggccttcgc catggcggag agcgtctcgt agttggaaca
     3781 cgccggacct atggtgcaga ctatcttcac tttcgtcatg agacaagact cctttcaatc
     3841 ctctttcttc aagaacacat ccggcttcaa ccgatttgaa acagctcctc cggaatgacc
     3901 tccgagagaa gaggggcaat accgccccgc ccgtcgactt ttatatcctg caggtagagc
     3961 acggtcgact cgctgtcgtt tcgcagctcc agcagaacgg gggattttcc cgggcaggct
     4021 ttcaatgccc ggatgaaatc cttgaacgac acgttctgta aaaagtccgc gaggacggag
     4081 atgcggacga aggaggtggt tccgttccgg ccgtcgtcct catcgaggag cgttatgctc
     4141 ttcgcgatga tgttcgtgtc tccccgctcc tccggaaatc cggagacgag acagacgcgc
     4201 ccgggctgta tcgtcccgcg cagcggctcc caggttcggg ggaagcagac ggcctctatc
     4261 cgcgattccg catcctcgat ctcgacgatt cccatcggat ccccgttctt ggtggtccgc
     4321 tccttcaccg agaggacgag gccgccgacc aggggaggta tgcgtttgct cctccatgac
     4381 cgcacttcgg caatggtaca gttcgagagc atacgcacct tcttctcgta ctgctcgaaa
     4441 gggtgcccgg agatgtataa ccccacggca tccttctcca tttcaagacg ttcaagcagc
     4501 gtgaaatcgg agacgtccgg gagatcggga ccggccgcgt cctcctcggg aagcgattcg
     4561 aagagcgaac actggttgct gttcgcacac ttcttctgcg ccgccgagat cagctcgggc
     4621 agcccttcaa ggagcttgcg tcggttgttt tccaggttgt cgaaggctcc ggcccggaca
     4681 aggttctcca cgacggcctt gttgacgacg cgcaggtcga cccggcagag gaagtcccac
     4741 atcgagagga agggggttcc cgtgttccgt gcggcaagaa tcgcctcgac tgcggtgtgc
     4801 cccgccttcg cgaccgctcc cagaccgaac cggacaacct cgccgaccgg cgtgaagctg
     4861 gccattgagg agttgacgtc gggcggaagg acgtcgattc cggagttccg gacctccctg
     4921 acgtacgcgg ccaggtcttc cttcttggag ccgatctggc tggagaggta ggcggccatg
     4981 aactccgacc ggtagttcgc cttgagccac gcggtctggt aggtgatcaa cgcataggct
     5041 gcgctgtgcg atttgttgaa gccgtaaccg gcgaactcct ggatgatgtc gaatatctcc
     5101 tgcgccgatt tttcgttgat attccgctgc gtacaccctt cgacgaactt cgcgcgctgc
     5161 tgctccatga cgtccgcctt cttcttgccc atggcgcgcc ggagaaggtc ggcctcgccg
     5221 agggagtacc cggcgagtat ggcggcgcac tgcatcacct gctcctggta gagaacgacg
     5281 ccgtaggttt cgcgcagcac gccttcgagc agcggatgga gatacttcac cgtagcgcgt
     5341 ccgtgcttgc actcgatata ctggtccacc attccgctgc cgagggggcc gggacggtac
     5401 atggcgagca cggcgataag gtcctcgaaa cagtccacct ggagcttctt gagcagctgg
     5461 cgcattccgg tcgattcaag ctgaaagact cccagagtgt cggccttctg cagcatttcg
     5521 taggtcttcc tgtcgtcgag cgggatgcgc tccatgtcgg ggagcgactt cccccccagc
     5581 gagatgttct gcagcgcctc ttcgatgacg gagagcgtcc ggagtccgag gaagtccatc
     5641 ttgacgaggc cgagcttctc gatgggctcc atcggatact gcgtaacaat ctggtcctgg
     5701 cctatgcggc gcacggggac gagatcagta ataggaacgg gggtgatgac cactccggcc
     5761 gcgtgctgcg aacagtggcg ggcaagccct tcgatgttgg aggcgacgtc gagcacgcgc
     5821 gccaccatcg ggttgctctt tttcaactcg gcaagctcgg gaacctgctc gacggcctcg
     5881 ccgatggagt ggattcccat cgggggaatg agccgcgtga tggcgacgac gtcgcgcacg
     5941 ggcatgccga gcaccctgcc gacgtcggtc accgccgccc tgctcttgag acgtccgaac
     6001 gtgacgatct gcgatacctt gtcgtgcccg tacttctcgg tgatgtaatg aagcagttcg
     6061 tcgcgcccct tgtcggagac gtccgtatc
//
LOCUS       DAOPKU010000131         6083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_13937_length_6083_cov_4.777206, whole genome shotgun sequence.
ACCESSION   DAOPKU010000131 DAOPKU010000000
VERSION     DAOPKU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13937_length_6083_cov_4.777206"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK849_05465"
     CDS             complement(<1..366)
                     /locus_tag="PK849_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE65608.1"
                     /translation="MKKVLALTLCFALVFGLTLTVAGQNAASAAEVVKIALIIESTVD
                     DKGWCQAMHDGILQAQKELPGRIEYSYSEKMKPVDAGSAVLQYVSQGYHIVIGHGAQY
                     KNLMLEMAEEYPEVTFAFGT"
     gene            634..867
                     /locus_tag="PK849_05470"
     CDS             634..867
                     /locus_tag="PK849_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65609.1"
                     /translation="MLQTKISLTPSLAEFLDDHRSYGFKDKSLMVRSALLRFKEELEL
                     ESLARSADLYAELYEQDEESRELTESALQGWPE"
     gene            864..1217
                     /locus_tag="PK849_05475"
     CDS             864..1217
                     /locus_tag="PK849_05475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPE65610.1"
                     /translation="MTLLKRGMVIDVNLSPTQGSETGKIRPCVVVTNDVYNAKVPVVQ
                     VVPVTEWNEKKGHIMTNVELLPSPENGLTKKSVADCLQTRPVDHRFRMVGVRGALTSE
                     EMHTIDDALRMVFSI"
     gene            complement(1308..2618)
                     /locus_tag="PK849_05480"
     CDS             complement(1308..2618)
                     /locus_tag="PK849_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPE65611.1"
                     /translation="MRKMGIMSIMLLSSLLCPATLCFASDYAFTLDESGIPALAGERS
                     AIVVISDLHLGMDDRYAETQENRAPLVDFLQKVRRAPNIKELVVAGDLVDEWFIPADT
                     ETYAGKTQKEFVQAVAANNKEVFDAFNSIILDGKIKVTYVPGNHDLLIDSESIQTILP
                     GMNEVRDVRGLGTYSPEGHAEIAIEHGHRYNFFCAPDPISNKDIAPGSILPPGYFFTR
                     IATLSVVEGKPEPGKIRPAVVPNSLGESQNLEFIYWQMWDALMKELPIKEDFEEKIIR
                     TNLDGFTQTYAMSDLMPRQATEGGVIDVNLFKGIQDTWDKRQELNNVAVKIPVREALM
                     KAASSAETDNQAVVQYFHNPASDKRIVIFGHTHESRVIPSETHDGKSAVYVNSGTWID
                     KNNNLPTMTFVVIAPQGSSRHAGLYHYSHEGAITTLDTLKLEGF"
     gene            complement(2670..3434)
                     /locus_tag="PK849_05485"
     CDS             complement(2670..3434)
                     /locus_tag="PK849_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65612.1"
                     /translation="MKRTDRIFALLLFFSLLLSGLPPLEASENADPSEWTFSTTSYVW
                     FSGLNGTLGVKGHKARVNLSFSDVLSNMDVSGMLTFQGHKGKQYFFFDGMYLDMSASE
                     QVSPQLFTDVGATTTRLQAAWGVRVKEWESSSLHVFGGLRYWNLKNELTFRTASAPLR
                     HFSESESWIDPLVGVRFATQLSPNLTFLAILDAGGFGIGSDFTWGGMLDLSWKLSERT
                     SLELGYRYLYVDYEKDGFVMDAYNDGIFIGFTWKLR"
     gene            3745..3942
                     /locus_tag="PK849_05490"
     CDS             3745..3942
                     /locus_tag="PK849_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HPE65613.1"
                     /translation="MQGTERADIKPGMKVLVVQKQDQRSGALTEGVVQSLLTKSPHHP
                     HGIKVRLESGVVGRVKEIIEE"
     gene            4098..>6083
                     /locus_tag="PK849_05495"
     CDS             4098..>6083
                     /locus_tag="PK849_05495"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPE65614.1"
                     /translation="MMTPSAIAPCRRYPKHASGSGGAKRSLSAVFRGPPVRQGGSARG
                     DRYAPLFACPPIWRRLAGALALLLICLFPLASGAEEKILSFVSAATVFKDSSLEVREE
                     ITVNVEGRQIQRGIFRDFPTKYKDSTGRAVTLGFSVKSALLGGREVPHTERSISDGVR
                     VYLGDPDGRAPIGEQTYTLVYVVTGALGFFDEHDELYWNVTGNEWAFTIDEARFSLSL
                     PGGASPAAVEFFTGWQGARGRDAKLLPDGAVRTTSPLPAGEGLTVAYAWPKGIVSPPE
                     EPFLRTFFARYHVHFLAALPLLLLFVYLLLWLRWGKDPPMPVIIPLFSPSPEKTPGFL
                     RYVSRMGMDDACFTAEILHLAVKGYIVIKKQEPEKKSDKALYSLQRTEKDPPGLPGAE
                     KLLLAALFSGERKEVTIRNESSVVLGDARKRLTKLFEKNGKGFFFKNTGIWCSGLFLL
                     VVGWLLMSAGGQGELAGASAFLTAAFAVVCSLGASESIASGKFRRYDGSDLLAFIVRR
                     FIPDFLGNLIILVVSGIVLAVLAAGALFFSGLWMVVVVLFELCAWVVSLLASAPLYVP
                     LLSFATAVVIVVFRELMTIRTPEGNRVLAEAEGLILYMGTAERRRFEMLNPPEETPEV
                     FETLFPYAFALDVAETWAQRFDSILKEQKYTPDWYEGG"
BASE COUNT         1167 a         1671 c         1887 g         1358 t
ORIGIN      
        1 ggtgccgaag gcgaaggtga cctccgggta ctcctcggcc atttcgagca tcaggttctt
       61 gtactgagcg ccgtgtccga tgacgatgtg gtatccctgg gagacgtact gaagaactgc
      121 ggaaccggcg tcgaccggct tcatcttctc gctgtagctg tactcgatgc gtcccggaag
      181 ctctttctgc gcctggagaa tgccgtcgtg catcgcctgg caccacccct tgtcgtcgac
      241 ggtgctctcg atgatgagcg cgatcttcac gacctcggcg gcggacgcgg cgttctgtcc
      301 cgcgacggtg agcgtcaacc cgaaaacaag agcaaaacag agcgtgagag ccaatacttt
      361 cttcatacag acccctcctt gaatttcgtt ttttaaaaag ctgctgaaat gtgcatccag
      421 tgtaaggggt ttcaaaagat tgtcaagcgc cgaacggcgc tccatatgcg gaacgagctc
      481 gtgtgtatgg aaagacgaag agcgtgccga ccgcgctcgc ggagcattta cttcgccttc
      541 gtggtccact ggatgggggc ttgttgacgg tcttgaaatt gggacctata attggggctc
      601 aataaatgct gctcgctttg gggaggtgtt gagatgctgc aaacaaaaat cagcctgaca
      661 ccgtcattgg cggagtttct ggacgatcat cgctcctatg gattcaaaga taaaagcttg
      721 atggtgcgca gcgcgctgct tcgtttcaaa gaagagcttg aactggaaag cctggcgcgt
      781 tccgccgacc tgtatgcgga gctctacgaa caggatgagg agtcccgcga gctcacggag
      841 agcgcgctgc aggggtggcc ggaatgacct tgctgaagcg gggcatggtg atcgatgtga
      901 atctttcccc gacgcaggga tcggagacgg ggaagattcg cccgtgcgtc gtggtgacga
      961 acgatgtgta caatgcgaag gtccctgtgg tgcaggttgt gccggtcacc gagtggaacg
     1021 agaaaaaagg gcatatcatg acgaatgtcg agctgcttcc ttcgcctgaa aacggcctga
     1081 cgaaaaagtc ggtggcggac tgtttgcaga cccgtcccgt ggatcaccgg ttccgtatgg
     1141 tcggggtacg gggtgcgttg acctccgaag agatgcacac gatagacgac gcattgcgaa
     1201 tggttttttc aatctaattt gctccggaca tgcgagaata agaaaaaaga gagaaaggcg
     1261 catttcgcgt cttcctctct ttctgttttc tgttccggcc ggtttcgtta gaagccttcg
     1321 agcttcagag tatccagcgt cgtgatggcg ccctcgtgcg agtagtggta gaggcccgca
     1381 tgccgcgagg agccctgcgg ggcgatcacg acgaacgtca ttgtcggcaa gttgttgttc
     1441 ttgtcgatcc acgtgccgga gttcacgtac acggcgctct tcccgtcgtg cgtctccgag
     1501 gggatcactc tggattcgtg ggtatgcccg aagatcacga tccgcttgtc ggacgcgggg
     1561 ttgtggaagt actggacaac ggcctgattg tcggtttccg ccgacgaagc agccttcatg
     1621 agcgcttctc tgacgggaat cttgacggcg acgttgttga gctcctgccg tttgtcccac
     1681 gtgtcctgta ttcctttgaa cagattcacg tcgatgacgc cgccctccgt cgcctgacgg
     1741 ggcatgaggt cgctcatggc gtaggtttgc gtgaatccgt cgagattggt cctgatgatc
     1801 ttctcttcga agtcttcctt tatgggcagc tctttcatca gcgcgtccca catttgccag
     1861 tagatgaact cgaggttctg gctctcgccg agggagttcg gaacgacggc cggtctgatt
     1921 ttgcccggct cgggtttgcc ttcgacgacg gagagcgtcg cgatgcgggt gaagaagtac
     1981 cccggaggca ggatcgagcc gggagcgatg tctttgttcg aaatgggatc cggcgcgcag
     2041 aagaagttgt agcggtgacc gtgctcgatg gcgatttccg catgaccttc cggagaatag
     2101 gttccaagcc cccgtacgtc gcgcacttcg ttcatcccgg ggaggatggt ctggatgctc
     2161 tcggagtcga tcagcaggtc gtgattcccc ggaacatagg tcaccttgat cttcccgtcc
     2221 agtatgatgc tgttgaaggc gtcgaacacc tctttgttgt tcgcggcgac ggcctgaacg
     2281 aactccttct gggtctttcc ggcgtacgtc tccgtatcgg cggggatgaa ccattcgtcg
     2341 acgaggtcgc ccgcgacgac gagctctttg atgttcggcg cgcgccggac tttctgaaga
     2401 aagtcgacga gcggagcgcg gttttcttga gtctcggcgt accggtcgtc cattcccagg
     2461 tggaggtcgc tgatgacgac gatagcggac cgttcgccgg cgagcgccgg aatgccgctc
     2521 tcatcaaggg tgaaggcgta gtcggacgcg aagcatagag ttgcggggca cagcagcgaa
     2581 gacaacagca tgatactcat gatacccatt tttctcatag tctcattccc tttcgtgcgt
     2641 gatatgatcg gtaaaagcgc gacggcgctc taccgaagtt tccacgtgaa gccgatgaag
     2701 atgccgtcgt tgtaggcgtc catcacgaag ccgtccttct cgtaatcgac gtagagatag
     2761 cggtagccca gctcgagcga cgtccgttcc gagagcttcc acgagagatc cagcattcct
     2821 ccccaggtga agtccgaccc gatgccgaag ccgcccgcgt cgagtatggc gagaaaggtc
     2881 aggttcggcg aaagctgtgt cgcgaagcgg acgccgacga gggggtcgat ccaggattcg
     2941 ctctcgctga agtgccggag cggggccgac gccgtcctga acgtcagctc gtttttgagg
     3001 ttccagtatc tcaggccgcc gaaaacgtgg agggaggacg actcccactc cttgacgcgt
     3061 actccccatg cggcctgaag ccgggtcgtg gtcgcgccga cgtccgtgaa caattggggg
     3121 gatacctgct cgcttgccga catgtcaagg tacataccgt cgaaaaagaa gtactgcttt
     3181 cccttgtgcc cttgaaaggt gagcattccc gagacgtcca tgttcgaaag aacgtcggag
     3241 aagctcaggt tgacgcgcgc cttgtgcccc ttcacgccca gcgttccgtt gaggccggag
     3301 aaccagacgt acgacgtcgt ggaaaaggtc cactcggagg gatcggcgtt ctccgaggcc
     3361 tccagcggag gaagtccgga aagaagaagg gagaagaaga gaagcaaagc aaaaatacgg
     3421 tccgttcgtt tcataaacgt cactccctct gcactttttt tgttcgctga gcagtgcgcg
     3481 ctctcgtgcg ctctttgtgc gcacaatcct ataaactata cgctatgtgc gtgattttgt
     3541 ccatatgttc aaagcctttt ttatgtttaa tgtattttta tatgtattct tttttcgtgc
     3601 ggccgcgggg gtgatgcgca ggcgctctct aaaggcgcct gccgcctttc gggattgttt
     3661 ccgctgttgt aagatggtgt tcttttcagg cgcattcagc ggcttgtttc ccctgagagc
     3721 gaaaaattga caggaggtga gtctatgcaa ggaacggaac gggccgacat caagcccgga
     3781 atgaaggtgt tggtggtgca gaagcaggac cagcgctcgg gcgcgcttac cgaaggagtg
     3841 gtgcagtcgc tgttgacgaa gagcccgcac catccgcacg gcatcaaggt ccgcctcgaa
     3901 agcggcgtcg tggggcgtgt gaaggagata atagaggagt aacagcgccg taaccaaggg
     3961 tacggcgcaa cgagacgcac ggctggcgct gctcgcttct gacgccgggc attgtgtgta
     4021 cgcgcatgga tgacgtgcac gaacatgata gaatacttta caagaggttc atcattctat
     4081 catgagggag gggaggcatg atgacgccgt ccgcgatagc cccctgcagg agatacccga
     4141 aacatgcgag cgggagcgga ggagcgaagc gcagtctctc cgcagttttt cgggggccgc
     4201 ctgtccggca aggaggaagc gcccgcggcg acaggtatgc tccgcttttt gcttgtcctc
     4261 cgatttggag acgtctcgcc ggagcgctcg cgctgctctt gatctgcctg tttccgcttg
     4321 cctcgggggc ggaggagaaa atcctctcgt tcgtttctgc ggcgactgtg tttaaggatt
     4381 cttcgctcga agtgcgcgag gagattacgg tgaatgtcga ggggcggcag atccagcgcg
     4441 gtattttccg cgacttcccg acgaagtaca aagactcgac tggacgcgcc gtcaccctcg
     4501 gcttttcggt gaagagcgct ttgctcggcg gacgggaggt tccgcacacg gaacgctcca
     4561 tttcggacgg cgttcgggtc tacctcggcg atccggacgg aagagcgcct atcggcgaac
     4621 agacgtatac gctcgtctat gtcgtgaccg gggcgctcgg ctttttcgac gagcacgacg
     4681 agctctactg gaacgtcacc ggcaacgagt gggctttcac gatcgacgag gcccgattct
     4741 ccctctcgct gcccggcggc gcgtcccctg ccgcggtcga gttcttcacg gggtggcagg
     4801 gagcgcgagg gcgggacgcg aagctgctgc cggacggcgc cgtccgcacg acgtcgccgc
     4861 ttcccgcggg cgaggggctt accgttgcgt acgcatggcc caaaggaatc gtgagccccc
     4921 ctgaagagcc ttttcttcgt acgttcttcg cacggtatca cgtacacttt ctggccgctc
     4981 tgccgcttct cctcctgttc gtctacctgc tgctctggct ccgctggggc aaagaccccc
     5041 cgatgcccgt catcatcccg ctcttctccc cgtccccgga aaaaacgccg ggctttctgc
     5101 ggtacgtgag cagaatgggc atggacgacg cctgcttcac cgccgaaatt ctgcatctcg
     5161 ccgtgaaggg gtacatcgtc atcaagaagc aagagcccga aaagaagtcg gacaaagcgc
     5221 tgtattcact gcaacggacg gaaaaggacc ctcccggact tccgggagcc gaaaagctgc
     5281 tgctcgccgc gctcttttcc ggggaacgta aagaggtgac catacggaac gagagcagcg
     5341 ttgttctggg cgatgcgcgg aagcgcctga cgaagctgtt cgagaagaac gggaaagggt
     5401 tctttttcaa gaacaccggt atttggtgct cggggctttt cctcctcgtg gtcggctggc
     5461 tgctgatgag cgcgggaggg cagggcgagc ttgcaggcgc ttcggccttc ctgacggcgg
     5521 cgttcgcagt cgtctgctcc ctgggcgctt ccgagagcat cgcctccgga aaattccgcc
     5581 ggtacgacgg aagcgacctg cttgcgttta ttgtccggcg cttcatcccg gattttttgg
     5641 gaaacctgat catcctcgtc gtctccggca tcgtgctcgc cgtgctcgcg gcgggggcgc
     5701 tttttttctc gggcctgtgg atggtggttg tcgtcctctt cgaactgtgc gcgtgggtcg
     5761 tctctttgct tgcgtcggct ccactctacg ttcctctgct ctcttttgcg acggccgtgg
     5821 tgatcgtcgt cttccgcgaa ctgatgacga tacggacgcc ggagggaaac cgcgtccttg
     5881 cggaggctga agggttgatc ctctacatgg gaaccgccga acggcgccgc ttcgagatgc
     5941 tcaatcctcc ggaggaaact cccgaggtct ttgaaactct cttcccctac gccttcgcgc
     6001 ttgacgtcgc ggagacctgg gcgcagcgtt tcgacagcat cctcaaggag cagaagtata
     6061 cgccggactg gtacgaaggg gga
//
LOCUS       DAOPKU010000132         6039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14052_length_6039_cov_4.229779, whole genome shotgun sequence.
ACCESSION   DAOPKU010000132 DAOPKU010000000
VERSION     DAOPKU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14052_length_6039_cov_4.229779"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /gene="dnaK"
                     /locus_tag="PK849_05500"
     CDS             <1..1384
                     /gene="dnaK"
                     /locus_tag="PK849_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPE65615.1"
                     /translation="NEPTAACLAYGENKQGEHKILVFDLGGGTFDVSILDVGEGVFEV
                     LATAGDNRLGGDDWDMRVVDWMVEQFRKSEGIDLKNDRMALQRLREAAEKAKVELSSM
                     PETTISLPFITANASGPKHLEMSLSRARFDEMTADLLERTVKPTRQAIQDSGLSAGEI
                     DKILLVGGSTRMPMVQRKIKELLGKEPTKGINPDECVAAGAAIQGAILAGDHKDIVLV
                     DVTPLSLGLETLGGVFTKIIERNTAIPVSKSQTFTTAGNNQTQVEISVLQGERAMAAD
                     NVKLGKFVLDGIPPAPRGIPQIEVTFNIDVNGILNVSAKDKGTGKAQNISIHSSNLSK
                     DDIERMKNQAEEFEEEDKKKRETAETKNEADGAAYAAERLLSENGDKMTPEEKGKITS
                     GMEELRRLLSGDDLASIRSAKESLEKALQEFSTRLYSQAQQQDDSSGTAGSASDGDTV
                     DAEFSDQGQA"
     gene            1400..2545
                     /gene="dnaJ"
                     /locus_tag="PK849_05505"
     CDS             1400..2545
                     /gene="dnaJ"
                     /locus_tag="PK849_05505"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPE65616.1"
                     /translation="MYSGGETDDLYQILGVPRDASAADIKKAYRQLVRKYHPDANPGN
                     KDAEERFKKINMAYEVLSDSQKRTQYDQFGTVGDMPGGGSPFEGFGGMGDIFGDIFDS
                     VFGGGMGRRRANPNAPRRGADLEMEVEVTLLEAATGAAKDFQIPRWESCGACGGTGAK
                     AGTSPESCSQCGGRGQVESRQQTPFGQFVSVNACPRCNGTGKIIKEKCPECGGQGRVR
                     TQHKVEVKIPAGVDTGTRLRISGEGEPGVNGGTSGDLYLVVSVQEHPDFKREGGDLHT
                     RLSISFPQAALGCTVDVPVLSGEPETLDIPAGTQPGKVFTLRGRGVPRLRGPRGKGDL
                     FVHVTVDVPTKLTEKQRALLEELAREMNVTVEDSGVFGKFFRNLFEG"
     gene            2683..3570
                     /locus_tag="PK849_05510"
     CDS             2683..3570
                     /locus_tag="PK849_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPE65617.1"
                     /translation="MNSSAESFWWYVTLEGRPGSEEVLASLADMSGSIGSEETAGEAS
                     VLLRAYFRSSQDLGFWLERLGGALSPWPEVRIADMGKIENKQWHTDWKEAFPPLAVGE
                     GLVVLAPWHKGNEPEGRMPLYIYPGSAFGTGYHESTQIVLTLLERTVVPGITAADIGA
                     GSGILSIAAVKLGAGHVRARDLDPAVLSEISHNLGLNEIPSGTVDLEEGNLLDGMDET
                     VDLLMANIIIEPLLEMLPNVPARLKEKGRALFSGLLVKERERFCAALEATGLRVIDEL
                     SLGDWWGVTVEPYAAPSRR"
     gene            3548..4903
                     /locus_tag="PK849_05515"
     CDS             3548..4903
                     /locus_tag="PK849_05515"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPE65618.1"
                     /translation="MLHHLAGKRIWICTLGCRSNQYEGEALANAFTEAEAILSDTPDC
                     DAAVLVSCTVTSVADKKCRQMIGRVRRTSPGAIIVACGCWAQHLPEEEAQSLGIHLLV
                     GNRKKQHVPFLLAQLFGDNSRRFSVCREDVLHSTEWDSLFLAKPLLHTRAFVKVQDGC
                     NHFCTYCAIPYARGFPVSRSPDDVLKEIRSIVFSDCREVVLTGVHLGLYGEYGEISLA
                     ELVRKVSLVEGLRRLRFGSIEPLGIDDGLLETLAETHAFQPHLHIPLQSGSDRVLSLM
                     NRGYSTAEYRDIVQRVRAFLGDDVHVSTDLLVGFPGEDERAFAETLAFLEECRFGKVH
                     VFPFSPREGTKAFSLPGRVPPQELSSRSKRALETGEKLLLEYSARWIGRTVPVLLESV
                     SSPESSDGAGWVFSGLSPSFLRVTGGGESGADPEFSAGDEVLVRITGSRDDSLKGRLI
                     Q"
     gene            4900..>6039
                     /locus_tag="PK849_05520"
     CDS             4900..>6039
                     /locus_tag="PK849_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPE65619.1"
                     /translation="MSAPVLGIDLGTRYALSAVPVKGKGVLVVPSRWGGGRTPSVTAL
                     TQSGWLAGEDAARSEMKNPSTTWWDMKRKLGSPWRARCGRASYTAEDVLVPLLTLLRE
                     DAEAFLGTFISSCVLTVPAFFSFAERSAMGRTARSSGFSSVRIVNEPTAAALAFGIMG
                     KFLILDYGAGTVDISVVESEGGVWQVLESAGLPASGGRDFDILLASSLNEKMKLPRLE
                     DNSALWRALLSEAESLKIALSSCASCEWTPPAGVSCPSLRVTREEFEDLIRPSIEQVT
                     TAVRRLWMRFKPSKLLLVGGGSRIPLLRALLEERVARPEHLNQCPDESVVTGAALYGI
                     SPTKERLLIDVLSQDLGILAADGTPVTLLEKDLHLPVRAERHFNSV"
BASE COUNT         1252 a         1641 c         1888 g         1258 t
ORIGIN      
        1 caacgagccg acggcagcat gcctcgccta cggagagaac aagcagggcg agcataagat
       61 ccttgtattc gacctcggcg gcggaacttt cgacgtgtcg atcctcgacg ttggcgaggg
      121 cgtgttcgag gtgctcgcca ccgccggcga caaccgcctt ggcggcgacg actgggatat
      181 gcgggttgtc gactggatgg tggagcagtt ccgcaaatcc gaggggatcg acctgaagaa
      241 tgatcgtatg gcgctccagc ggctccgaga ggctgcggag aaggcgaagg tcgagctctc
      301 ctcgatgccc gagacgacga tttcgcttcc tttcatcaca gcgaacgcct ccggccccaa
      361 gcaccttgag atgagcctct ccagagccag gttcgatgag atgacggccg atcttctgga
      421 gcgtacggtg aaacccacgc gccaggcaat tcaggactcc ggtctgtccg ccggggagat
      481 cgacaagatc cttctggtcg gcggttccac tcgtatgccg atggtgcagc ggaagatcaa
      541 ggagcttctc gggaaggagc ctacaaaggg catcaacccc gacgagtgcg ttgcggcggg
      601 cgctgcaatt cagggagcca ttctcgcggg cgaccacaag gatattgttc tcgttgacgt
      661 aacgcctctc tccctcggat tggagacgct cggcggcgtg ttcacgaaga tcatcgaacg
      721 caacaccgcc atccctgtct cgaagagcca gacgttcacc actgcgggga acaatcagac
      781 ccaggtcgag atcagcgtgc tccagggaga acgagccatg gccgccgaca acgtgaagct
      841 gggcaaattc gtcctcgacg ggattcctcc ggcgccccgc ggcattccgc agatagaggt
      901 caccttcaat atcgacgtca acggtattct caacgtaagc gcgaaggaca aggggacggg
      961 caaggcgcag aatatctcca tccactcgtc gaacctctcg aaggacgaca tcgagcggat
     1021 gaagaatcag gcggaggagt ttgaagaaga ggacaagaag aagcgtgaga cggccgagac
     1081 gaagaacgaa gcggacgggg ccgcatacgc ggcggagcgc cttctctcgg agaacggaga
     1141 caagatgacc cctgaagaga aggggaaaat cacgtcggga atggaggagc tccgcaggct
     1201 tctctccggt gatgatctcg cctccatccg ctcggcgaag gagagcctcg aaaaggcgct
     1261 ccaggagttc tccaccaggc tctattctca ggcgcagcag caggacgact cttccggaac
     1321 ggcggggagc gcctccgacg gcgacaccgt ggacgctgag ttcagcgacc agggccaggc
     1381 gtaggggagg tgaccgtgta tgtacagcgg aggagagacc gacgacctgt atcaaatact
     1441 gggggtgccc cgggacgctt ccgccgctga cataaagaaa gcctaccgcc agctggtacg
     1501 aaagtaccac cccgacgcca atccgggcaa caaagatgcc gaggagcgtt tcaagaagat
     1561 caatatggcg tacgaagtgc tgagcgactc ccagaagcgg acccagtacg atcagttcgg
     1621 aaccgtgggc gatatgccgg gcggagggag tccgttcgaa ggcttcggcg gtatggggga
     1681 catcttcggc gatatattcg acagcgtctt cggcggtgga atggggcgga gaagagcgaa
     1741 cccaaacgct ccccgacggg gggccgacct ggagatggag gtcgaagtca cgctgttgga
     1801 ggcggcgacc ggggcggcga aagactttca gattccgcgc tgggaatcgt gcggcgcgtg
     1861 cggcgggaca ggcgcgaagg ccggtacgtc gcccgagagc tgctcacagt gcggcgggcg
     1921 cggacaggtg gagagcaggc agcagacgcc tttcggccag tttgtctcgg tgaatgcctg
     1981 tccccggtgc aacggcacgg gaaagatcat taaggagaag tgtccggagt gcggaggcca
     2041 gggaagggtt cgcacgcagc acaaggtcga ggtgaaaatt ccggcgggcg tggataccgg
     2101 aaccaggcta cgaatatccg gagagggcga gccgggggta aacggaggaa cctcgggcga
     2161 tctctatctc gtcgtgagcg ttcaggagca ccccgatttc aagagggagg gcggagacct
     2221 gcatacccgt ctttccatat ctttccctca ggccgctctg gggtgcacag ttgatgtacc
     2281 cgtgctttcc ggcgagcccg agacgctgga catccctgcc ggaacacagc cgggaaaggt
     2341 attcactctc cgcggcaggg gcgttccgcg ccttcgcggt cccagaggca aaggagatct
     2401 gtttgtccat gtaacggtgg atgtgccgac gaagctgacg gagaagcagc gcgctcttct
     2461 tgaggagctg gccagggaga tgaatgtcac ggtggaggat tccggggttt tcgggaaatt
     2521 tttcaggaat ctcttcgaag ggtaagtttt ttttcgagac gctcgttcag taaaagagta
     2581 ttcgccctgt aaaaacggcg cttcttctgc tacactcttt atcagcacga aaagggaggc
     2641 tgcactagcc tcccttattt catcttccgg gaggaattcg gcatgaacag ttcagcagaa
     2701 agcttctggt ggtatgtcac tctggaaggg cggcccggga gcgaggaggt tctggcctct
     2761 cttgcggata tgtcgggaag cattggttca gaggagactg ccggagaagc ttccgtcctc
     2821 ctgcgggcat actttcgcag ctcccaggat ctgggttttt ggctcgaacg ccttggcggg
     2881 gcgctgtccc cgtggccgga agtcaggatc gccgatatgg ggaaaataga gaacaaacag
     2941 tggcataccg actggaagga agccttccct cctcttgcag taggagaagg gctcgtcgtt
     3001 ctggctccct ggcacaaagg gaacgaaccc gaaggccgga tgcccttata tatctatccc
     3061 gggtccgcgt ttggcacggg gtaccacgag agcacgcaaa ttgtgctgac gcttctggaa
     3121 cgtactgtcg tccccggaat cactgccgcc gacatcggag caggttccgg catcctctcc
     3181 attgccgcgg tcaagctggg agccggacat gtcagggcgc gggacctcga tcccgctgtg
     3241 ctctccgaaa tcagtcataa tctgggactg aacgaaatcc cctccggaac ggtggacctt
     3301 gaggagggga atcttctcga cggaatggac gaaaccgtcg acctcctcat ggcgaatatt
     3361 attatcgaac cgctcctcga aatgcttccg aatgtccccg cacgactgaa ggagaagggg
     3421 cgcgctcttt tttcaggttt gcttgtaaag gagcgtgagc gtttctgcgc tgcgcttgag
     3481 gcaacaggat tgcgcgttat cgacgaactc tcacttggcg actggtgggg ggtaacggta
     3541 gaaccctatg ctgcaccatc tcgccggtaa gagaatctgg atctgcacgc ttggatgccg
     3601 gtccaaccaa tacgagggcg aggctcttgc caatgccttc accgaggcgg aagcaattct
     3661 gagcgacacc cccgactgcg atgcggcggt tctggtgagc tgtaccgtca cttcggttgc
     3721 ggataaaaaa tgccggcaga tgatcgggcg ggtacgccga acgtcccccg gcgcgattat
     3781 cgtcgcgtgc gggtgctggg cgcagcatct tccggaggaa gaggcgcagt cgctcgggat
     3841 tcatctgctc gtcggcaacc ggaaaaaaca gcatgttccg tttcttcttg cacaactctt
     3901 cggggataat tcccggcgtt tttcggtatg tcgggaggat gtccttcaca gtacggagtg
     3961 ggattctctt ttcctcgcga agccgctgct tcatacccgc gccttcgtca aggtgcagga
     4021 tggctgcaat catttctgta cctactgtgc aattccctat gctcgaggat ttcccgtcag
     4081 tcgctctccg gacgatgtac tgaaggagat tcgttccata gttttttccg actgccgtga
     4141 ggtcgtgctc accggggtgc atctggggct gtacggcgag tacggtgaga tatctttggc
     4201 ggagcttgtc agaaaagtat ctttggtcga ggggcttcgc cgtcttcgct tcggttcgat
     4261 cgaacctctg ggaatcgacg acggcctgct tgaaacgctt gcagaaactc acgctttcca
     4321 accgcatctc catattcccc ttcagagcgg aagcgaccgg gtactctctc ttatgaacag
     4381 agggtattcg acggcggagt atcgtgatat agtgcaaaga gttcgtgctt ttctcgggga
     4441 tgatgtccac gtcagtaccg atttgctcgt cggcttcccc ggggaggacg aacgcgcatt
     4501 cgcagagact cttgctttcc ttgaagagtg ccgcttcggg aaggttcatg tcttcccgtt
     4561 ctccccccgt gaagggacga aagccttctc tctccctgga agagtgcctc ctcaagagct
     4621 ctcctcaaga agcaagaggg ctcttgaaac aggagaaaaa ttgttgctcg aatacagtgc
     4681 ccgctggata ggtcgaactg ttccggtgct cttggagagc gtctcgtccc ccgaatcctc
     4741 cgacggcgcg ggatgggtat ttagcgggct ttctccgtcc tttctccgcg tgacgggagg
     4801 aggggaatcc ggagccgatc ctgaattttc agcaggagat gaggtccttg tcaggattac
     4861 cggctcacgt gatgattctt tgaaaggtcg cctgattcaa tgagcgcccc tgtccttggt
     4921 attgatcttg gaacgcgata tgcgctctcc gcagtccctg ttaagggaaa gggagtcctg
     4981 gtggtaccga gccgctgggg aggaggacga acgccttcgg taacggcctt gacgcaatcg
     5041 gggtggctcg ccggggagga tgcggcgcga agtgaaatga aaaacccgtc cacaacctgg
     5101 tgggacatga aacgaaaact tggttctcca tggcgggcac gatgcggtcg cgcttcctac
     5161 acggcggaag acgtgcttgt tcctctgctg acgttgttgc gggaggacgc cgaggcgttc
     5221 ctcggaacct ttatctcctc ctgcgttctt accgttcccg cttttttcag cttcgcggag
     5281 cgatctgcca tgggaagaac ggcgcgttcc tcgggctttt catccgttcg catagtaaac
     5341 gaaccgacgg cagctgcgct tgcattcggc ataatgggga aatttctcat cctcgattac
     5401 ggcgcgggga ctgtggacat ttctgttgtg gagagcgaag gcggcgtatg gcaggtgctt
     5461 gagagcgccg gtttgcctgc ctcgggaggg cgggattttg acattcttct tgcatccagt
     5521 ttgaacgaga agatgaagct gccgcgtctt gaagacaact ccgccttgtg gagagcgctt
     5581 ctttctgaag cggaatctct taaaattgct ctttcctcct gcgcttcgtg tgaatggacg
     5641 cctccggcgg gagtttcctg cccttctctt cgcgtaacgc gcgaggagtt cgaagatctg
     5701 attcgtccat ccatcgagca ggtgacgacg gcggtgcgcc gtctctggat gaggttcaag
     5761 ccttcaaagc tcctgctggt gggtggagga agcaggatac cgcttcttcg cgccctgctg
     5821 gaagagcgtg tcgccaggcc tgaacatctc aatcaatgcc ccgacgagtc cgtcgttacg
     5881 ggcgctgctc tctacggcat atctcctaca aaggagcgtc tcctcatcga cgtcctctcg
     5941 caggacttgg gaatattggc tgccgacggt actccggtga cgctcctgga aaaagacttg
     6001 catcttcctg ttcgtgcgga aaggcatttc aacagtgtc
//
LOCUS       DAOPKU010000133         6038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14057_length_6038_cov_5.022898, whole genome shotgun sequence.
ACCESSION   DAOPKU010000133 DAOPKU010000000
VERSION     DAOPKU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14057_length_6038_cov_5.022898"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="ftsY"
                     /locus_tag="PK849_05525"
     CDS             complement(<1..888)
                     /gene="ftsY"
                     /locus_tag="PK849_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPE65620.1"
                     /translation="MSFFEKLKKSLQGVRKKWSSGLSGIFSGSAIDEEFWDRLEEVLI
                     SGDVGVTLSLRLIEDLKKEARARGAKTPEELFQFFADILGARLSAVPLMGQPVTPDKN
                     SLTIVILVGVNGSGKTTTAGKLASQWTKEGFRVVFAAADTFRAAAIDQLKVWGERSGV
                     RVIAQQQGSDAAAVAFDALSAAKSSRAHVLLVDTAGRLHSKHNLMEELGKIYRVLEKE
                     RGDAPLETLLVLDAVMGQNGLAQAQTFNKILPLSGVVLSKYDNTAKGGILLAIAEELK
                     MPVRYVGLGEGEDDLRPFDI"
     gene            complement(924..2552)
                     /gene="ricT"
                     /locus_tag="PK849_05530"
     CDS             complement(924..2552)
                     /gene="ricT"
                     /locus_tag="PK849_05530"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPE65621.1"
                     /translation="MSIHLAIFGKPRYLGLLNIEAPFPSKGTWLVVETMRGSELALLG
                     GPLSDEQEARYRASCNDDASDGQVKGGEPILQEISLVRTALDEDLAAAELEREEENEV
                     LIRARKLLRNHNLSMKLVDVEYLLDKKKLFFYFTSEQRVDFRAYVRDLAKEFKTRIEL
                     RQIGVRDEAKTVKGIAPCGRECCCSHWLHRFTPICIKMVKEQNLALNPTKISGICGRL
                     MCCMSYEHTSYSSLWKTLPNPGAKIRTPAGNYIVEGVELSSNSVRVRRPDGGSLLIVI
                     EDFPRFKETVLEGKEWEDSSRTPRDFSSAKSLFDSPASKGTRSFSDDSSRTPRKRAAE
                     SGGHEKVQEQGSLAKEKKTEGKPAVSGTEKNKREKEKKLQEGLLAPEEVAKPAKKKRR
                     RKKPSASGDNKTTPVGTQQPRQQERGDGKQTESKYSEEASSGGRPSRKHASPELNQSN
                     LSAEKTDGAPHSPENTTAPSARRSSSSRRRRSGKGGGNRDTHEQNKSPEQKTEGSESK
                     NASSGGAQQKRQKNKPHPRPKEESGESRKEKGPE"
     gene            complement(2524..3333)
                     /locus_tag="PK849_05535"
     CDS             complement(2524..3333)
                     /locus_tag="PK849_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65622.1"
                     /translation="MSVFSSVLNTEGISEQRGASWKDSSAWKRLLFLASRGAFPHSCA
                     VVIDENLHMHAALGIVRVLLCSNGSGDDDCRVCSAWSGERHPDLLYCGAPSKPPSIDE
                     CREIIDAMAYRPVVSSKRVAVVFSADRMMLPAANSLLKLAEEPPEYVHMLFLLTDEKL
                     FLPTLRSRSWSVSLPVPRSGESMPPPQTEEEWIRWIEKNAKSEVDELIVLFAPWISYE
                     VERGAYDKAAALERIRLLIQTKRLSRTMVLDLIVLALKEGIVFEHSFGDLW"
     gene            complement(3309..3743)
                     /locus_tag="PK849_05540"
     CDS             complement(3309..3743)
                     /locus_tag="PK849_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HPE65623.1"
                     /translation="MRVKKTSKETLSGGEFSKTSKRSGQLPSRESTASVFSASLEDAE
                     IRDLLDRLDIIGRKLSIFPAEALLIQYRKLVSELVRRAVAGLRVRRDMKWRRGDKNFF
                     VIVEKTDSMLDELESVFAREGERTRMLQLMEEIKGCLFSLLF"
     gene            complement(3740..4354)
                     /gene="tmk"
                     /locus_tag="PK849_05545"
     CDS             complement(3740..4354)
                     /gene="tmk"
                     /locus_tag="PK849_05545"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPE65624.1"
                     /translation="MFITFEGIDGCGKTTQAALLANFLEGKYRGNRRVVWTREPGGWD
                     GGSWIRERILEGDFSHPMSELFLFLVDRCEHVRKEILPVLEGGGVVICERYTDSTLAY
                     QSWGRGIPSEKVETLFEWCSFPRPHKTFWLDVPVKTAMERVCLRGGRDRFEAEGYSFL
                     ERVREGFSSLAKREPQRICTVEASSEPQALASRILSLVEVSLSR"
     gene            complement(4359..>6038)
                     /locus_tag="PK849_05550"
     CDS             complement(4359..>6038)
                     /locus_tag="PK849_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HPE65625.1"
                     /translation="VEIVSVIPGKGSPKHLIMIKASGSVIAATGGIAAGMSGSPVFIN
                     DKLIGAIGYGWNFSEHTLGLVTSFSDMASIWSWPEKIVSIPPAPALDDADKKKDQEDE
                     SQAGQEADNGKEDKEEDKLVVPVAAEQLSSPLFINGISTRKALETAALLGRDNFIPGG
                     GFTDAVPVEMNATMLPGDAITVLLAWGDVTIGATGTLTAVAPDGRFIAFAHPFLGRGA
                     VNYPVARAYIHKVIPSLESPFKVGSPLQIVGTVTQDRPQGIGGRIGYFTPSISATLDF
                     TDAERNIKERKRFHVVPDPFIGAKLTAGVFTGLIDDLWGRKGQGTATLTLNVQGRGLI
                     SGWTRTNMFFSDSDLAAAALKETSELLDVVFLNPFAEVYPLGITVTTEFSPMPKLMYI
                     EGVNLKGESFSSGETVEVEVLIRPYRKKQVKKSFTITIPKDASGICEILVRGGGIEPL
                     NQSAIVQGWKTIENFRQLFTEMSALETNNELIIEFNYEKRSKDGKGRSDEDEPVSKEE
                     QELLSELKQRRLKDGTLQIFKSEFVVEGLLRKLITVTPGNSSQSDEPETES"
BASE COUNT         1368 a         1634 c         1425 g         1611 t
ORIGIN      
        1 gatatcgaat ggacgaaggt cgtcttcccc ttctccaagg cctacatacc gtaccggcat
       61 ttttaactcc tctgctatag caaggagaat gccgcctttc gcagtattgt cgtacttgga
      121 gaggacaacg cctgaaagag gcagaatttt attgaacgtc tgcgcctgag ccagtccgtt
      181 ctgtcccatc acggcatcca gtacgagcag cgtttcgaga ggagcgtcgc cccgttcctt
      241 ttcgagcact ctgtaaatct ttccgagttc ttccataagg ttatgtttcg agtgaagacg
      301 tccggcagta tcgacaagaa gaacgtgcgc acggctcgat tttgcggcag agagtgcgtc
      361 aaaggctact gcggctgcat cgctcccttg ttgttgcgcg atgacgcgta ctcccgaacg
      421 ctccccccat acttttaatt gatcaatcgc ggcggcacga aacgtgtcgg cagccgcaaa
      481 gacaactcgg aagccttctt tcgtccattg ggaagcgagt ttacctgctg ttgtggtttt
      541 cccgcttcca ttgacgccga caagaatgac tatggtcaac gaatttttgt ccggcgttac
      601 cggctgtccc atcagaggaa cagcagaaag gcgggctcct aagatgtccg cgaagaattg
      661 aaaaagctct tccggcgtct tggcgccgcg tgcacgcgcc tcttttttca aatcctcaat
      721 cagacgcagt gagagcgtga cgcctacatc tcctgagata aggacttctt cgagacgatc
      781 ccagaactcc tcatctattg cgctgcccga aaaaattccg gagagtccgc tgctccattt
      841 tttgcgtact ccctgcagac tttttttgag cttttcaaag aaagacatgt actgtcaggc
      901 ctcctcctcg ggacgtgcag ccgttattcc ggcccctttt ctttgcggga ttctccagat
      961 tcttctttag ggcgggggtg cggtttgttt ttctgtcgtt tttgttgcgc tccgcccgag
     1021 gaagcgttct ttgattcgga tccttcagtt ttttgttccg gagacttatt ttgctcgtgt
     1081 gtgtctctgt ttcctccgcc ttttccggaa cgtcttcttc tggaactcga agaccgtctg
     1141 gcggaagggg cggtagtatt ctcaggagaa tggggagctc catcggtttt ctctgccgat
     1201 aaattgctct ggttaagctc aggcgacgca tgtttcctgg acggtcgacc accggaagag
     1261 gcttcttccg agtattttga ctctgtttgt tttccgtctc cgcgttcctg ttgacgcggc
     1321 tgttgggttc caaccggtgt ggttttgttg tcgccggaag ctgaaggttt ctttcttcgt
     1381 ctcttttttt tcgccggttt ggctacctct tcaggtgcaa gaagaccttc ctgaagcttt
     1441 ttctcttttt ctcgtttgtt cttttctgtt ccggaaaccg caggtttgcc ttccgttttt
     1501 ttctcttttg cgagagatcc ttgttcctgc actttctcat gcccgccgga ttcagctgct
     1561 ctcttgcgag gagtgcggga cgagtcgtct gagaatgaac gtgtgccttt tgacgccggg
     1621 ctgtcaaaaa gagacttcgc cgatgagaaa tcacggggcg tacgagaaga gtcctcccat
     1681 tccttccctt ctaagacagt ctctttgaaa cgcggaaaat cttcaattac gatgagaagg
     1741 cttcccccgt ccggacggcg tacgcgtacg gagttggatg agagctcaac tccctcaacg
     1801 atataattgc cggcgggagt gcggattttt gcacccggat tcgggagcgt tttccagagg
     1861 ctgctgtacg atgtgtgctc ataggacatg caacacatca gccttccgca gatgcccgat
     1921 attttcgtcg gattcagagc gagattctgc tcctttacca tctttatgca gattggtgtg
     1981 aacctgtgaa gccagtggct gcagcaacat tctctgccgc acggcgcgat ccctttcact
     2041 gtttttgcct cgtcgcgcac gcctatctga cggagttcga tacgggtttt gaattctttc
     2101 gccagatccc ggacatacgc acggaagtca actctttgtt cggacgtaaa atagaaaaag
     2161 agtttcttct tgtcaagaag atattcaacg tcaacaagct tcatagagag gttatgatta
     2221 cgaagcagtt ttctcgccct gataaggact tcgttttctt cttcccgttc gagttcagca
     2281 gctgcaagat cttcatccag cgccgtccgt acgagcgaga tttcctggag aatcggttcg
     2341 cctcccttga cctgaccgtc agaggcatcg tcattacaag aagcccggta acgcgcttct
     2401 tgttcgtccg agaggggacc gccaagaaga gccagttcag aacctctcat cgtttcgaca
     2461 acgagccagg ttcccttgga agggaaaggg gcttctatgt tgagaagccc aagatatcga
     2521 ggtttaccaa agatcgccaa atgaatgctc aaaaacaata ccctccttga gagccaatac
     2581 aataaggtcg agaaccatgg ttcgggaaag acgtttcgtc tggatcaaaa ggcgtattcg
     2641 ctctagcgct gccgccttgt catacgctcc ccgttctact tcataggata tccatggagc
     2701 aaagagcaca atcagctcgt cgacttcaga cttggcattt ttttcaatcc accgaatcca
     2761 ctcttcctct gtttgcggcg gcggcatgct ctcacccgaa cgaggcacag gaagagaaac
     2821 actccacgaa cggcttctca gtgtgggcag aaagagtttc tcgtccgtaa gtaaaaaaag
     2881 catatgaaca tactccggcg gttcttctgc cagttttaac agactgtttg ccgcaggaag
     2941 catcatcctg tccgcagaaa aaacaactgc aactcttttt gatgatacca ccggacgata
     3001 cgccattgca tcgattatct cacgacactc gtcaatagaa ggaggttttg agggagcgcc
     3061 gcaatacaaa agatccggat gcctctcgcc ggaccaggct gaacagaccc tgcaatcatc
     3121 atctcccgaa ccgttactac agagcagcac gcgaacaatt ccgagcgctg cgtgcatatg
     3181 gaggttctcg tcgatcacta ctgcgcacga gtgagggaag gctcctcttg atgccaaaaa
     3241 cagcaaacgt ttccatgccg aagagtcctt ccatgaagcg ccgcgctgct cggagattcc
     3301 ttccgtgttc agaacagaag agaaaacaga catcccttga tctcctccat taattgcaac
     3361 atcctggttc gctctccttc ccgagcgaaa acagattcca attcatcaag catggagtct
     3421 gtcttttcca caataacgaa gaaattctta tcccctctac gccatttcat atcccgccgt
     3481 acacgtaaac cggctactgc ccgtcgcacc agctcggaaa caagtttacg gtactgaata
     3541 aggagcgcct ccgcagggaa aatagaaagt tttcttccga taatgtcaag gcgatccaaa
     3601 agatcgcgga tttcagcatc ctcaagagaa gccgaaaaaa cggaagcagt cgattcacga
     3661 gaaggcaact gcccgcttct tttcgatgtt ttggaaaatt ctcctccgct tagtgtttct
     3721 ttggaagttt tttttaccct caacgggaca aagacacctc cacaagagag agaattcttg
     3781 acgcaagagc ttgcggttct gaagaagcct cgaccgtgca aatcctttga ggctctctct
     3841 tggccaagga agaaaatccc tcgcgaaccc tctcaagaaa cgaataccct tccgcctcga
     3901 aacggtcgcg tcctccgcgg aggcagactc tctccatcgc agtttttaca gggacatcaa
     3961 gccagaatgt cttatgcggt ctcgggaaag aacaccattc gaacagcgtc tctacctttt
     4021 ccgacggaat tcctcttccc caggattggt atgccagtgt agagtctgtg taccgttcgc
     4081 aaataacgac gccgccgccc tcaagtacgg gcagaatctc cttgcgaacg tgctcgcaac
     4141 ggtccacaag aaagagaaac aactcgctca ttggatgaga aaaatctcct tccaaaatgc
     4201 gttcccgtat ccacgagccg ccgtcccacc ctccgggttc tcttgtccat actacgcgcc
     4261 ggtttccccg gtattttccc tcaaggaaat tggcaaggag tgcagcttgc gttgtttttc
     4321 cgcatccgtc aataccttcg aatgtgataa acatctgatt aactctccgt ttccggctcg
     4381 tctgattgcg atgagttgcc gggcgtgaca gtgatcagtt ttcgaagaag cccctcgacg
     4441 acaaattccg atttgaatat ctgaagcgtc ccatctttta aacggcgctg tttcagctcg
     4501 ctcagaagct cttgttcttc ttttgaaaca ggctcgtctt catcggaacg gccttttccg
     4561 tcttttgacc gtttctcgta attaaattcg atgataagtt cattgttcgt ctcaagtgcg
     4621 gacatctcgg tgaaaagctg acggaaattt tcaatggttt tccagccttg gactattgcc
     4681 gattggttca gcggttctat cccgccgcct cgaacaagaa tttcacagat accggaagca
     4741 tccttcggga tggtaatcgt aaatgatttc tttacctgtt tcttcctgta agggcgaata
     4801 agaacttcaa cttcgacagt ttctccggag gagaacgatt ctcctttgag attcacgcct
     4861 tctatataca taagcttcgg catcggggag aattcggtag ttacagttat ccccagggga
     4921 tacacttcgg caaaagggtt caagaacaca acgtcgagca gctcgcttgt ctcttttaaa
     4981 gcggccgctg caagatcgct gtctgaaaag aacatatttg tcctcgtcca tccgcttatc
     5041 aaccctcttc cctgcacatt aagggtgagg gtcgccgtac cttgtccctt tcgtccccac
     5101 agatcgtcga tcagcccggt aaagactccc gcagtcagct ttgcgcctat gaaaggatcc
     5161 ggaacgacgt gaaaacgctt cctttctttt atattccgtt cagcatccgt aaaatcgaga
     5221 gttgcagaaa tggaaggcgt aaaatatccg attcttcccc ctattccctg cggtctgtcc
     5281 tgagtaaccg tcccgacaat ttgcagcggg ctccctactt taaacggaga ctctaaactg
     5341 gggattactt tatggatgta tgcccttgcc acaggatagt ttacagcccc tctcccgagg
     5401 aaagggtgag cgaaggcgat aaaccttccg tcaggcgcca cagctgttaa tgtgccggta
     5461 gcaccgatgg ttacatctcc ccatgccaga agcactgtaa tggcgtcccc gggcagcatt
     5521 gtggcgttca tctcgaccgg aacagcatcg gtgaagccgc cgcccggaat aaaattgtcc
     5581 cttccgagaa gggcggcagt ttcaagggcc ttgcgagtgc tgataccatt gataaagaga
     5641 ggcgaggaga gctgctctgc ggctaccggt actacaagtt tatcctcttc tttgtcttcc
     5701 tttccgttat ccgcttcttg acctgcctga ctctcgtctt cctgatcttt tttcttatct
     5761 gcatcatcaa gtgccggggc tggaggaatg gacacgattt tttccggcca actccagatg
     5821 cttgccatat cgctgaaaga ggtgaccaat ccgagcgtgt gttcactgaa attccagcca
     5881 tatccaatcg ccccgatcag cttgtcgttg ataaatacgg gactgccgct catgcctgcg
     5941 gcaattccgc ctgtcgccgc gattacagag ccggaagcct taatcattat caggtgttta
     6001 gggcttcctt ttcccgggat cacgcttaca atttcaac
//
LOCUS       DAOPKU010000134         6035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14064_length_6035_cov_4.522241, whole genome shotgun sequence.
ACCESSION   DAOPKU010000134 DAOPKU010000000
VERSION     DAOPKU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14064_length_6035_cov_4.522241"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..554
                     /locus_tag="PK849_05555"
     CDS             54..554
                     /locus_tag="PK849_05555"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE65626.1"
                     /translation="MQYIISITTTTGVVMLMMYCIMMLSRIYVQERLPAMIISSLTSL
                     SSNKYVLMLMINLFMIIIGMIMDDVSAMLLCTPILLPVVVSLGIHPIHFAAIIGTNLG
                     LGNVTPPTAPTLYFGGRMAKTPVSQMMKPAMIFILFAWLPTLLAVTFLPELALWLPRL
                     VLGGRL"
     gene            567..1772
                     /locus_tag="PK849_05560"
     CDS             567..1772
                     /locus_tag="PK849_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HPE65627.1"
                     /translation="MNMEYLKDKPVAVLGGGATARGHAACTALAGREVRLYELPEFFE
                     GLGCIASKREIRVSGRQESLYGFKREGLAKIDVVTDDMAKAVRGAGIIVVSFPAVGYA
                     AFLERLIPHLEDGMVVHFTTANFGSLLFRKMLREAGCTKKIVVGEWSSQPYGIRIKNM
                     GGQQMPEVSVNYWAITLRGAALPMTDQDAFFESKRYLPSLDSVEHPVHGDTVADVGFS
                     NVNPILHCPGSILGVGAMENWGVVYGENKYDFSIYSHAYCPSISKVQLALYREECAIA
                     EAMGVGIQDFPEKAFFSRSNILGSEHMGEKFVVPFEEQYTLAMGTGPFSIYNRYITED
                     IPVGCHIYLELAKKFGIEVPVIESMITLASVMTGTDFRAAGVTLEDLGIAHMNKEALN
                     AWLREGIYA"
     gene            1765..3444
                     /locus_tag="PK849_05565"
     CDS             1765..3444
                     /locus_tag="PK849_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE65628.1"
                     /translation="MPEERGSVLKKYAESGLCRLLLDMLRIPSVSPSPEHENRVTRLL
                     YDTLAVLPYFREHPEDLRLLPLEDDPFGRNFLFAFVRRPGPTAETVILAGHADVVSAE
                     ACGELAPFAFDPEEYTRRLEDVALPEDARRDLESGEWLFGRGVADMKSGLAAGIELLA
                     EAASDPGSLGANLLALAVPDEENNSAGMLAASAQLARRQKEEGLRYRACIELEPTFAG
                     GEWALPSIYLGSIGKINTFFFCAGKETHVGEYYEGFGAAPVISRINLELEGNPDYADS
                     LDGRAYPPFGCMRQTDLRREYSATIMTRAFSFYSYLTATKLPGRILGEMRAIAEKALR
                     EAIEAHERNAESFAKMNGAGRSDAKWNSMALSYEELRRMAEAKLGAGFPGFVEEVLSR
                     TPSGADERTKAVALVESMVEACALPGPLVVFGFLPPWYPHRANLGLSEGERRVERAAR
                     ETVREASERFGLTVETRPFFEGVSDLSYCGFQGEAGEMATFAANMPGWKRLYSLPTEA
                     LAELDIPILNFGPLGKDAHKNTERLHLPYFMEVFPKLLRSLVRRVAEAGER"
     gene            3706..4251
                     /gene="pyrR"
                     /locus_tag="PK849_05570"
     CDS             3706..4251
                     /gene="pyrR"
                     /locus_tag="PK849_05570"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPE65629.1"
                     /translation="MTERDMERVLRRIAVEIVERNKGLDDVVLLGIQRRGVHLAARLR
                     EIFKAEEKVKVPAGELDITLYRDDISTLADQPVVHSTSLPGDITGKRVVLVDDVLFTG
                     RTIRAALDAITDLGRPERVQLAVLVDRGHRELPIAADYVGKTIPTSRSENVEVRVKEL
                     DGTDGVVICERNGGAEHGVEA"
     gene            4235..5161
                     /locus_tag="PK849_05575"
     CDS             4235..5161
                     /locus_tag="PK849_05575"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPE65630.1"
                     /translation="MEWRHKDLISLEGWTREELHFFLEQADYMEELMNRPIKKVPALR
                     GKMVVNCFFENSTRTRVSFELAEKMLSADVVNWSASGSSTGKGETLRDTVWTLEAMGA
                     DAVVVRHGAVGVPDYLSKKLHRASVFNAGDGTNGHPTQALLDLHSARKKLGNLDGAKM
                     AMIGDVLHSRVARSDIQAFRKMGVTVTLSGPATLMPEDTGSLGVEYVSDAREAARGAD
                     ILYFLRIQRERQEDGLIPSIDEYHRRWGATDSLVALASPKAAVMHPGPMNRGVEIASS
                     VADGPNSVILGQVRSGVAVRMALLYLCLGGAR"
     gene            5158..>6035
                     /locus_tag="PK849_05580"
     CDS             5158..>6035
                     /locus_tag="PK849_05580"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPE65631.1"
                     /translation="MSILLKNAKIFDGRSLGSECEDILVKDGVIARRGSGLPEDGAEV
                     YDLGGKLLCPGFIDIHVHFRDPGYEWREDTESGARAAAAGGYSTVVTMPNTEPAVDNP
                     VTVEYLLARGKRAGGARVLPSGCVSKGRQGKQLAELGLMAESGAVMFTDDGSPVWDAK
                     LLKTALLYSKDLDVRIMEHPEEPTLTAKAHVNEGVVSASSGMKGWPASAEVIDIQRGI
                     ALSRETDAPIHFTHVSTELGVDAVRQAKKEGLPVTCDVTPHHLSLDENCILVSRFSST
                     YKVNPPLRTRKDVKAL"
BASE COUNT         1119 a         1792 c         2050 g         1074 t
ORIGIN      
        1 gcgcgaggtc gaagaacagc atggccagga cgtagatgcg gctgagcatc atgatgcagt
       61 acatcatcag catcacgacg accaccggcg tcgtgatgct gatgatgtac tgcatcatga
      121 tgctcagccg catctatgtc caggaacggc ttccggccat gattatttcc tctcttacct
      181 ctctgtcgag caacaagtac gtgttgatgc tgatgatcaa cctcttcatg atcatcatcg
      241 gcatgatcat ggacgatgtg agcgcgatgc tgctctgcac cccgatactg cttccggtcg
      301 tggtctccct ggggattcac cccatccatt tcgccgcgat catcgggacg aatctcggcc
      361 tgggaaacgt gacgccgccg actgcgccga cgctctactt cggcgggcgc atggcgaaga
      421 cgcccgtttc gcagatgatg aaaccggcca tgatcttcat ccttttcgca tggctgccga
      481 cgcttctggc ggtgaccttc ctgccggaac ttgcactctg gctgcctcgt cttgtgctcg
      541 gcggccggct ctaggaggcg tccgatatga atatggagta tttgaaagac aaaccggtag
      601 cggttctcgg cggcggggcg acggcgcgcg gtcatgccgc ctgtacggcg ctcgccgggc
      661 gggaggtccg cctttacgaa ctgcctgagt tcttcgaggg gctggggtgt atcgcgagca
      721 aacgcgaaat acgagtcagc ggaaggcagg agagcctgta cggattcaag cgcgagggac
      781 tcgcgaagat cgacgtggtc accgacgata tggcgaaggc cgtccgcggc gcggggatta
      841 tcgttgtcag cttccccgcc gtggggtacg cagcctttct tgagcgtctg atcccgcacc
      901 ttgaggacgg aatggtcgtg catttcacga cggcgaactt cggctcgctg ctcttccgga
      961 agatgctccg cgaagctgga tgcacgaaga agatcgttgt cggcgagtgg agcagtcagc
     1021 cctacggcat tcggatcaag aacatgggag gccagcagat gcccgaggtg agcgtcaact
     1081 attgggcgat cacgctgcgc ggggcggcgc tgccgatgac ggaccaggat gccttcttcg
     1141 aatcgaagcg ctacctcccg tcgctcgact ccgtggagca ccccgttcat ggggataccg
     1201 tcgcggacgt cggtttcagc aatgtcaacc ctatcctgca ctgcccgggg tccatactcg
     1261 gcgtcggcgc gatggagaac tggggcgtcg tctacggtga gaacaagtac gatttctcca
     1321 tttactcgca cgcatactgc ccttcgatct cgaaggtgca gctcgccctc taccgcgaag
     1381 agtgcgccat cgccgaagcg atgggtgtcg gcattcagga cttcccggag aaggccttct
     1441 tttcgaggtc gaacattctg gggtcggaac acatggggga gaagttcgtc gtccccttcg
     1501 aggagcagta cacgctggcg atgggaacgg ggccgttctc catctacaac cgctacatca
     1561 ccgaggatat tcccgtcggc tgtcacatct atctggagct ggcgaaaaag ttcggaatcg
     1621 aggttcccgt catcgagtcc atgatcacgc tcgcctccgt catgaccggg acggacttcc
     1681 gggccgccgg cgtgacgctg gaggacctgg gaatcgccca catgaacaaa gaagcgctga
     1741 acgcctggct tcgggaggga atctatgcct gaggagaggg ggagcgttct gaaaaaatac
     1801 gccgagagcg ggctctgccg tctgctgctc gacatgctgc ggattccgag cgtctccccc
     1861 tcgccggagc atgaaaaccg cgtgacgcgc ttgctgtacg acacgctcgc cgtgcttccg
     1921 tactttcggg agcaccctga ggatctccgc ctccttccgc ttgaggacga cccgttcgga
     1981 cgaaatttcc ttttcgcatt cgtacgcagg ccggggccga cggcggagac ggtcattctg
     2041 gcggggcacg cggatgtggt gagcgcagag gcgtgcggcg agcttgcccc cttcgcgttc
     2101 gacccggagg agtacacgcg gcgtctggag gacgttgcgc ttcccgagga cgcccgtcgc
     2161 gacctcgaaa gcggggagtg gctcttcggg cggggcgtcg ccgatatgaa gagcggactt
     2221 gcggcgggta tcgaactcct ggcggaggcg gcgtcggatc ccggaagtct cggcgcgaat
     2281 ctgctcgcgc tcgcggttcc cgacgaggag aacaactccg caggaatgct cgccgcatcg
     2341 gcgcagctgg cgcgcaggca aaaggaagag gggctgcgct accgcgcgtg catcgaactc
     2401 gaacccactt tcgcaggggg ggagtgggcg cttccgtcca tctaccttgg cagcatcggg
     2461 aagatcaaca cctttttctt ctgcgccgga aaggagacgc acgtcgggga gtactacgag
     2521 gggttcggcg cggcgccggt gatctcccgg atcaacctgg agcttgaagg caacccggac
     2581 tatgcggatt cgctcgacgg gagagcgtac cccccgttcg gctgtatgcg ccagacggac
     2641 cttcggaggg agtactcggc gacgattatg acgcgcgcct tctccttcta cagctacctc
     2701 acggcgacga agctgccggg gcgcatcctc ggcgaaatgc gcgccattgc ggagaaggcg
     2761 ctgcgggaag cgatagaggc gcacgagcgc aacgccgaat cgttcgcgaa gatgaacggg
     2821 gcgggacgct ccgacgcaaa gtggaattcc atggccctct cctacgaaga gctgcgccgc
     2881 atggcggagg caaaactggg cgcggggttc cccggcttcg tcgaagaggt tctttcccgg
     2941 acgccttccg gggccgacga acggacgaag gccgtcgcgc tcgtcgaaag catggtcgag
     3001 gcgtgcgcgc tgcctgggcc gcttgtcgtc ttcggcttcc tgccgccgtg gtaccctcac
     3061 cgcgcgaacc tcggcctgtc agaaggggag cgcagggtag agcgtgcggc gcgcgaaaca
     3121 gtgcgggagg ccagcgagcg gttcgggctg acggtggaaa cgcgcccgtt cttcgagggg
     3181 gtttccgacc tcagctactg cggttttcag ggcgaggcgg gggagatggc gacgtttgcg
     3241 gccaacatgc ccggctggaa acggctctac agcctgccga cggaggcgct tgcggagctg
     3301 gacatcccga tcttgaactt cggcccgctc gggaaggacg cccataagaa tacggagcgt
     3361 ctgcaccttc cgtacttcat ggaggtcttt ccgaagctcc tgcgttctct cgtcaggaga
     3421 gttgcggaag cgggagaaag gtaactttct tcctcgaatt tttctctgca accgcttgca
     3481 ttgatttcga gagtgtgcta tcattccccc agcttgaaga gaggggggag gcgccatgtg
     3541 gagtactatc tgtcggccgc gtgttcgcag cgcttctctt cctgttctcg gacacaatgt
     3601 cggagccttt ctcccgtcgg gagaggggct ctttttttac ggaagggaga tgggatcgtg
     3661 gtgaaagcgc agtccggaaa tgtgtggacg gaaaaagcga cggtcatgac ggagagggat
     3721 atggagcggg tgctccggcg catcgccgtc gagatcgtcg agcggaacaa ggggctcgac
     3781 gacgtggtgc ttctgggaat ccagcggcgc ggcgtgcatc ttgcggcccg cttgagggag
     3841 atcttcaagg ccgaagagaa ggtgaaagtc cctgcgggcg agctggatat cacgctctac
     3901 cgcgacgaca tctcgacgct tgccgaccag cccgtggtgc acagcacctc gctccccggc
     3961 gacatcacgg gaaagcgcgt ggtgctggtg gacgacgtcc tcttcacggg gcggaccatc
     4021 cgggcggcgc tcgacgcgat caccgacctg gggcgtccgg agcgcgtgca gctcgccgtg
     4081 ctggtggacc gggggcacag ggagctgccc atagccgcgg actacgtcgg aaagacgatc
     4141 cccacgtcgc gctcggagaa cgtcgaggtg cgggtgaagg agctcgacgg cacggacggc
     4201 gttgtcatct gcgaacggaa cggaggtgcg gagcatggag tggaggcata aggacctcat
     4261 cagcctcgaa ggctggacga gggaggagct gcacttcttc ctggagcagg cggactatat
     4321 ggaagagctg atgaaccgtc cgatcaagaa ggtccccgcg ctgcgcggca agatggtggt
     4381 gaactgcttc ttcgagaact cgacgcggac cagggtctcc ttcgagctgg cggagaagat
     4441 gctctccgcg gacgtggtga actggtcggc ctccggatcg agcacgggca agggcgagac
     4501 gctgcgcgac acggtctgga cgctcgaagc gatgggcgcg gacgctgtgg tggtgcgcca
     4561 cggcgcggtg ggcgtccccg actacctctc gaaaaagttg cacagggcct ctgtcttcaa
     4621 cgcgggggac gggacgaacg gccatcccac gcaggcgctg ctcgacctgc acagcgcccg
     4681 gaagaagctc ggcaacctcg acggcgcgaa gatggccatg atcggcgacg tgctccacag
     4741 cagagtggcg cgctccgaca tccaggcctt ccggaagatg ggcgtcacgg tgacgctcag
     4801 cggaccggcg acgctgatgc ccgaagatac cggttcgctc ggcgtcgagt atgtgtccga
     4861 cgcgcgcgaa gcggccaggg gggccgacat cctctacttc ctgcggatac agcgcgagcg
     4921 gcaggaggac gggctcatcc cctccatcga cgagtaccac cggcgctggg gcgcgacgga
     4981 ctcgcttgtc gccctcgcct cgccgaaggc cgcggtgatg catccggggc cgatgaaccg
     5041 cggagtggag atcgcctctt ccgtggccga cggcccgaac agcgtgattc tcgggcaggt
     5101 ccggagcggc gtcgccgtcc ggatggccct gctctacctt tgccttggag gtgcgcgatg
     5161 agcatactgc tgaaaaatgc gaaaattttc gacggaagaa gccttggcag cgagtgcgag
     5221 gatatcctcg tcaaggacgg cgtgatcgcc cgcagaggct ccggactccc cgaggacggc
     5281 gcggaggtct acgacctcgg cgggaagctg ctctgccccg gcttcataga tattcatgtt
     5341 cacttccgcg acccggggta cgagtggcgt gaggatacgg agagcggcgc gcgcgctgcg
     5401 gcggcgggag ggtactccac cgtcgtgacc atgccgaaca cggagcccgc agtggacaat
     5461 ccggtgactg tcgaatatct gctcgcccgc gggaaacggg ccggaggcgc acgggtgctg
     5521 ccctccgggt gcgtgagcaa ggggcggcag ggcaaacagc tcgccgaact cggcctgatg
     5581 gcggagtcgg gagcggtgat gttcaccgac gacggctccc cggtatggga tgcgaagctg
     5641 ctgaagacgg cgctgctgta cagcaaggac ctcgacgtgc ggatcatgga gcaccccgag
     5701 gagcccacgc tgaccgcgaa ggcgcacgtg aacgagggcg tggtctccgc ctccagcggc
     5761 atgaaggggt ggcccgcctc ggcggaggtc atcgacatcc agcggggcat cgcgctgagc
     5821 cgcgagacgg acgcacccat ccacttcacc cacgtgagca cggagctggg ggtggacgcg
     5881 gtacggcagg cgaaaaaaga gggacttccg gtgacgtgcg acgtgacgcc gcaccacctc
     5941 tcgctcgacg agaactgcat cctcgtcagc cgcttctcct cgacgtacaa ggtgaacccg
     6001 ccgcttcgga cgcgcaagga tgtcaaggcg ctctg
//
LOCUS       DAOPKU010000135         6015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14139_length_6015_cov_4.476174, whole genome shotgun sequence.
ACCESSION   DAOPKU010000135 DAOPKU010000000
VERSION     DAOPKU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14139_length_6015_cov_4.476174"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PK849_05585"
     CDS             complement(<1..1125)
                     /locus_tag="PK849_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPE65632.1"
                     /translation="MRNHQDNLRNEPVGSLLFRLSFPAALGMAVQASYSVVDAFFVGW
                     GVGHEGIAAVSMAFPLQMLLMAAAQTLGVGGASLLSRSLGAERRKRAERTLGTVFTCT
                     WILGILFAAVAIPSAPFVLYFLGAGEGVLPLASEYARALFRGAPFFAFSIVANNFVRA
                     EGNASFAMMTMLISAGINTLLDPLFIFGFEWGVRGAALATVAAQTATALWLAWYYLAR
                     KSAVRLRMKNLPPRAILLKETLSVGAAAFFRQAASSLSLIVVNGSLAALGGGAAVAAY
                     GIVNRLLLFAVMPVYGIVQGLLPIVGFNYGAKQFCRVLSAMRRSIASATVLCTAGAAA
                     LFFFPGESVDFFTSSPEVRRAGIDAARILAPGMLVTGFQIM"
     gene            1260..2189
                     /locus_tag="PK849_05590"
     CDS             1260..2189
                     /locus_tag="PK849_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HPE65633.1"
                     /translation="MQEVKKVITEVGTNEWQVVVFFLGDQTFAINVDKTREILRWPGC
                     RTIPESHPSMIGITSVRGEVLPLLDLRIHLGIEAKTPLDSSKVIIAEFNEIKLGFVVD
                     AVERIYRINSEELDSTLTGTFLGEYVLYVIKRDERNVLLLDYEAIVQMVNPTLSQRYT
                     IDVEKTAELTTPLGDLDQYRILVAEDSPLIRKQIADVLGQGGFHNLVLTSDGKEAWDK
                     MNTLGEHYDLLITDVEMPRLDGLALVRRVKEHNDLKKIPIVVFSSIMAQDIKVKAASV
                     GAEAQITKPEIPDMVEHVCRLLLANKEKKGSSK"
     gene            2186..2857
                     /locus_tag="PK849_05595"
     CDS             2186..2857
                     /locus_tag="PK849_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65634.1"
                     /translation="MTLLPYWDSNVDMEIASWFPLRMDYEPVSVNAHHSRLLSDIAAF
                     SLRMNSEFFCLTSLQSGYVGTLLRDSMIDPGRLNRIELGFTMLFGYSTSMESLLSSLL
                     DDESDVEAGVSFCCLWDRDIMLESFKAIVLVSSNPLFDEDEFLSRLAALLEKNSVPSQ
                     ANLPFFAEKFGYRRFFIPYETDNLVHLEISRLLYEMEFEETRKWVGPSPEVAFPPFFG
                     INLTV"
     gene            2971..4173
                     /gene="rpoD"
                     /locus_tag="PK849_05600"
     CDS             2971..4173
                     /gene="rpoD"
                     /locus_tag="PK849_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPE65635.1"
                     /translation="MENDMLNEKEILEDKEILEEKDVSKKDELSREDMAEYLGRVREL
                     IAEGHGKGFVTQKDIERHIPLENWSSEILDNVVSNLMELGIEVMDDDAKEPAPAQASA
                     AAADDDAVAPPSYDLELGKLDDIPLTDPVRMYLREIGKVPLLDAAEEVELAKRVEAGE
                     DLAKKKIVDANLRLVVSIAKKYIGRGMLFLDLIQEGNLGLIRAVEKFDYRKGFKFSTY
                     ATWWIRQAITRAIADQARTIRVPVHMVETINKMVRISRQLVQRLGREPTDDEIAAEME
                     IEPIKVEEIRRIAQLPVSLETPIGEEEDSQLGDFIEDRELPNPEEAAAGHLLHEQIEE
                     MLDALSEREREVLHYRFGLEDGRSYTLEEVGKRFGVTRERIRQIEAKALRKLRHPSRS
                     KKLKDFLD"
     gene            4307..5476
                     /locus_tag="PK849_05605"
     CDS             4307..5476
                     /locus_tag="PK849_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPE65636.1"
                     /translation="MRLFSIVIICTLFALYSYIYFKLRSAIGPGWKWTALYTLLSFFI
                     IMGSRILWIVNLEAYPGLRKILSCSVYMGMAFFFILFTAFFFIDLLRFLVWLTDLLLS
                     ACFSDLFPSPKMRAVLAIGFAFFACTYGWFEALAVRPVYITIATDRLPRGVDKLRIAH
                     ITDVHLGWVIQEERLDRILDVVRAAQPDMLVSTGDLVDGDMEEREAEVALFRGLNLPL
                     GMYAVTGNHEYYAGIDQAIAFKEHAGMQLLRNEALRVGGIILAGMDDPTALRFGGTPT
                     PEIDVLAPLPENRFVLLLKHQPTVDPDSIGLFDLQLSGHTHRGQIWPFYWLTRMVYEY
                     RPGLRALAPSKSGEAYASGAARESRVFVSNGSGTWGPPIRFFAPPQVGIIDIVRK"
     gene            complement(5512..>6015)
                     /locus_tag="PK849_05610"
     CDS             complement(5512..>6015)
                     /locus_tag="PK849_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPE65637.1"
                     /translation="ERLKDDLLTLTRWGANLLAVPCNTAHFFIDRFKDELPVPLVHII
                     EETVRAAATLSPKGAWLTATLGTMGSGLYQKEAERQGYSFFIPPEEAQRQVQRSIELV
                     KSGKKDEGGLLLKGVVEGLWRERDLPVCAACTELPLAYDASGLPQERTVSSLGALCGA
                     CLRRIYG"
BASE COUNT         1277 a         1892 c         1742 g         1104 t
ORIGIN      
        1 catgatctga aatcccgtga ccagcatacc cggcgcgagt attcgcgcag cgtcgatgcc
       61 ggcgcgccga acctcagggg aggacgtgaa gaagtccacg ctctccccgg ggaagaagaa
      121 gagggctgcc gcgcccgctg tgcacagaac cgtggcggaa gcaatggagc ggcgcatcgc
      181 ggagagcacc ctgcagaact gcttcgcccc gtagttgaac cccacgatcg ggagcagccc
      241 ctggacgatg ccgtagacag gcatgacggc gaagagcagc agccggttca cgatgccgta
      301 ggcggccacc gcggcgcccc ctcccagcgc ggcaagcgat ccgttcacca cgatgaggga
      361 gaggcttgag gccgcttggc gaaagaacgc cgcagctccc acggagagcg tctctttcag
      421 gaggatggcg cgcgggggga gattcttcat ccgcagcctc actgcgcttt ttcgggcgag
      481 atagtaccac gccagccata atgccgtcgc cgtctgcgcc gcgaccgtgg ccagggccgc
      541 gccgcgcacc ccccactcga agccgaagat gaacagcggg tcgagcagcg tgttgatccc
      601 cgccgaaata agcatcgtca tcattgcgaa cgacgcgttc ccctcggccc ggacgaagtt
      661 gttcgccacg atggagaacg cgaaaaatgg cgcgcccctg aacagagccc gcgcatactc
      721 ggaggcgagc ggaagcaccc cctcccccgc gcccaggaag tagaggacga acggcgcgga
      781 cggaatcgcg acggcggcga agagaatccc caaaatccac gtgcaggtga agactgttcc
      841 gagcgtccgt tccgcacgct tccttcgttc cgcgccgagg ctccgcgaaa gaagcgacgc
      901 cccgccgacg ccgagagtct gggcggccgc catcagcagc atctgaagcg gaaaggccat
      961 ggaaacagcg gcgattcctt cgtgccccac gccccagccg acgaagaaag cgtccaccac
     1021 gctgtacgaa gcctgcacgg ccatgccgag cgcggcggga aaggaaagcc ggaacaacag
     1081 cgaacctaca ggttcgttgc gcagattatc ctggtgattc cgcacccgca ttcccctttc
     1141 tcaatttgaa ttcgattcca agtatagcag aagtaccgag gaggggcttg tggtccgagc
     1201 cttcatggga tatagtaagc acggaaaaca atccgttccg ttcggaggag ggagcgtcca
     1261 tgcaggaagt taagaaagtg atcaccgagg tgggaaccaa cgaatggcag gtcgtggtgt
     1321 tctttcttgg ggaccagacc tttgcaatca acgtggacaa gaccagggag attcttcgtt
     1381 ggcccggatg ccgcaccatc cccgaatccc atccgtccat gatcggcatc acttcggtcc
     1441 gcggagaggt gcttcccctt cttgatctgc gcatacatct cggcattgaa gccaagaccc
     1501 cgctcgactc gagcaaggtc atcatcgctg aattcaacga gatcaaactt ggattcgtgg
     1561 tggacgccgt cgagcggatc tatcggatca actccgaaga gctcgactcc acactgacgg
     1621 gaaccttcct gggagagtat gtcctctacg tcatcaagcg cgacgaacgg aacgtgctgc
     1681 ttcttgacta tgaggccatc gtccagatgg tcaacccgac gctctcgcag cgctacacca
     1741 tcgacgtcga aaagaccgcc gagctgacga cccctctcgg cgatctcgac cagtaccgca
     1801 ttctcgtcgc ggaggactcg ccgctcatcc gcaagcagat tgccgacgtg ctcgggcagg
     1861 gaggcttcca taatctcgtg ctcacaagcg acggcaaaga ggcctgggat aagatgaaca
     1921 ccctcggaga gcactacgat ctgctcatca ccgacgtcga gatgccacgc ctcgacgggc
     1981 ttgcgctcgt ccgccgcgtc aaggagcaca acgatctgaa gaagattccg atcgtcgtct
     2041 tctcgtccat tatggcgcag gacatcaagg tcaaggccgc gagcgtcggc gccgaagcgc
     2101 agatcacgaa gcccgaaatt ccggatatgg tggagcacgt gtgccgcctt ctgcttgcga
     2161 acaaggagaa gaagggatcg tcgaaatgac cctgcttccc tattgggatt ccaacgtcga
     2221 catggagatc gcttcgtggt ttcctctcag gatggactac gagccggtct ccgtcaacgc
     2281 gcaccacagc aggcttttga gcgacatcgc agccttctct cttcgaatga acagcgaatt
     2341 tttctgcctt accagccttc agagcggcta tgtcggcacg ctactccggg acagcatgat
     2401 cgaccccgga cggctgaacc gcatcgagct gggtttcacc atgcttttcg gctactccac
     2461 gtcgatggag agcttgctct cctccctgct tgacgacgag agcgacgtgg aagcgggcgt
     2521 ctccttctgt tgcctgtggg acagggacat catgcttgag agcttcaaag ccatcgtcct
     2581 tgtgagcagc aaccctctgt tcgacgaaga cgaatttctc tcacgccttg cggcgctcct
     2641 cgaaaagaac agcgtgcctt cgcaggcgaa tctgcctttt ttcgccgaga agttcggcta
     2701 ccggcggttt ttcatcccct acgagacgga taacctcgtc cacctggaga tcagccgcct
     2761 tctgtacgag atggagttcg aggaaacccg caaatgggtc ggcccctctc cggaggtggc
     2821 gtttcctcct ttttttggta tcaatctgac tgtatagtgg tataatatat gtccgtcgga
     2881 aaagagaaga agaacacacg cgacgagccg gtgtcccgag aggaacatta ggcccgtcat
     2941 tgtcgctttg aagaggggac tggttgttcg atggagaacg atatgctcaa cgagaaggaa
     3001 attctggagg ataaggaaat ccttgaggag aaagacgtct ccaaaaagga cgagctttcg
     3061 cgggaggata tggccgaata cctcggaaga gtgcgggaac tcatcgccga ggggcacggt
     3121 aaggggtttg taacccagaa ggacattgag cggcatatcc ctctcgagaa ctggagctcc
     3181 gagattctcg acaacgtcgt ctccaacctc atggagctcg gaatcgaagt aatggacgac
     3241 gacgccaagg aaccggctcc cgcgcaggcc agcgccgcgg cagcagatga tgacgcagtg
     3301 gcgccgccct cctacgacct cgaactcggt aagctggacg acatccctct taccgacccg
     3361 gttcgcatgt acctccgcga gatcggcaag gtgccgctgc tcgacgccgc cgaggaggtc
     3421 gaactggcca agcgcgtgga ggccggagaa gatttagcga agaagaagat cgtcgacgcc
     3481 aacctgcgcc ttgtggtgag catcgccaag aagtacatcg ggcgcggaat gctcttcctc
     3541 gacctcatcc aggaggggaa cctcggcctg atccgcgcgg tggagaagtt cgactaccgc
     3601 aaggggttca agttcagcac ctacgccaca tggtggattc gccaggccat caccagggcg
     3661 atcgccgacc aggcgcgcac catccgcgtc cccgtccaca tggtggagac catcaacaag
     3721 atggtccgca tctcccgcca gctcgttcag cgcctggggc gcgagccgac cgacgacgag
     3781 atcgccgccg agatggagat cgagccgatc aaagtggagg agatacgccg tatcgcgcag
     3841 ctccccgtct ccctcgaaac gcccatcggc gaggaggaag acagtcagct cggcgacttc
     3901 atcgaggaca gggagcttcc caaccccgag gaggccgcag ccgggcacct gcttcatgag
     3961 cagatcgagg agatgctcga cgccctctcc gagcgcgagc gcgaagtgct gcactaccgc
     4021 ttcggcctgg aagacggccg ttcgtacact ttagaagagg tcggcaagcg cttcggcgtc
     4081 acccgcgaac gcatccgcca gatcgaggcc aaagcgctgc gcaagctccg tcaccccagc
     4141 cgaagcaaga agctgaagga tttcctcgac taaccgctga aagagagcgc cgacgcgtac
     4201 ccggatcctc tcacgagagt ccgggtactt tttctcgata cgtgcgccgg ctgctataat
     4261 aaaacccgga aacgaatgtt ctctcgagac aaggaggagt gatgaagtga ggctcttttc
     4321 catagtgatt atctgcacgc tctttgcgct gtactcctac atctatttca aactgcgcag
     4381 cgccatcggt cccggatgga agtggacggc gctctacacg ctgctctcct ttttcatcat
     4441 catggggtcg aggatcctct ggatcgtcaa cctggaggcg tacccgggac tcaggaaaat
     4501 tctttcctgc tccgtctaca tgggaatggc tttcttcttt atcctcttta cggccttctt
     4561 ttttatcgac ctcctgcgtt tcctcgtctg gctgaccgat ctgctgctct ccgcctgctt
     4621 cagcgatctc ttcccctctc cgaaaatgcg cgcggtgctc gcgatcggat tcgctttctt
     4681 cgcctgcacc tacggctggt tcgaagccct tgcagttcgc cccgtgtaca tcactatcgc
     4741 cacagacagg ctccccaggg gagtggacaa actccggata gcgcacatca cggacgtgca
     4801 tctgggctgg gtcatccagg aagaacggct cgaccgcatt cttgatgtgg taagagccgc
     4861 gcagcccgac atgctggtga gcaccggcga ccttgtcgac ggcgacatgg aggagcgcga
     4921 ggccgaggtt gcactcttca gagggctgaa tctgccgctg gggatgtacg ccgtcaccgg
     4981 aaaccacgaa tactacgcgg gcatcgatca ggcgatcgcc ttcaaggagc acgccggaat
     5041 gcagctgctt cgcaacgagg cgcttcgcgt cggcggcatc atcctggcgg gaatggacga
     5101 ccctacggcg ctacgcttcg gcggaacgcc cacaccggag atcgacgtcc tcgccccgct
     5161 tcccgagaac cgtttcgtcc tgctcctcaa gcaccagccc acagtggatc cggattccat
     5221 cggtcttttt gatctccagc tctccggcca cacgcacagg ggccagatat ggcccttcta
     5281 ctggcttaca aggatggtct acgagtacag gccgggacta cgcgccctgg cgccgtcgaa
     5341 gagcggagag gcctacgcct ccggcgccgc ccgcgagagc cgcgtcttcg tcagcaacgg
     5401 atcgggaacg tgggggccgc cgatacgctt cttcgcgccg ccccaagtgg gcatcatcga
     5461 catcgtgaga aaataaaaaa gcgcccccgt gtcgaacagg gggcgctttt gctacccgta
     5521 gatccttcga agacaggccc cgcagagcgc ccccaggctg gagacggttc tctcctgcgg
     5581 cagccccgag gcgtcatagg cgagaggcag ctccgtacac gccgcgcaca ccgggagatc
     5641 gcgctcgcgc cacagcccct ccacaacgcc cttcagcagc agccccccct cgtccttctt
     5701 gccgcttttc accagttcga tgcttcgctg cacctgccgc tgcgcctcct ccggaggaat
     5761 gaaaaacgag tacccctgcc gttccgcttc cttctgatac aggccgctgc ccatcgttcc
     5821 aagcgtcgcc gtcagccagg cccccttcgg actcagtgtc gccgccgccc ttaccgtctc
     5881 ctcgatgatg tgcaccagag ggacgggaag ctcgtccttg aaacggtcga tgaaaaagtg
     5941 cgccgtgttg cacggcacgg ccaaaagatt cgccccccag cgcgtcagcg tcagcaggtc
     6001 gtccttgaga cgctc
//
LOCUS       DAOPKU010000136         5981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14259_length_5981_cov_4.323321, whole genome shotgun sequence.
ACCESSION   DAOPKU010000136 DAOPKU010000000
VERSION     DAOPKU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14259_length_5981_cov_4.323321"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1831)
                     /locus_tag="PK849_05615"
     CDS             complement(<1..1831)
                     /locus_tag="PK849_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPE65638.1"
                     /translation="MINALRSLSLPRRGIIDTEDVHQKETPVEKTETENESGLPSRFV
                     LVRPPSHGIPADVLQRIEETQLSEERLPQEDTEGFEEEKNGALFGFFKRKRKTLPEDE
                     PLLTENSIVALTPHEARSEALPEQPDAALPEVFETHSDREEMSEVDDTDTDTDIEMEE
                     DVFLLRRPEENISGQGLQTELPEDDAVVAKIEQDSEDVLTDPHALPDIEFHVVEEEGR
                     VRSGTFPPPPEIFGPRQEMETSDDNETVREQASSIITTLADFGVSAEMADIVIGPTVI
                     QFQLQLAPGIKVSKVAGLSNDLAVALTVPSLRVEAPIPGKPYVGVEIPNPKRKGIALR
                     TILESRVFLDSENDLPLAMGVRVDSRPLVVGLEDLPHLLVAGTTGSGKSVFVTSCITG
                     LCTHRSPEELRLILIDPKRVELSIYEHLPHVLAKPVVSPKKAVQALAWAVREMEHRYE
                     VFARARVRNLKSYNQNVLPKAKLPHIVIVVDELADLMFTAQKDVEDYICRLAQMARAT
                     GIHLVLATQRPSVNVITGLIKANIPARVAFTLPSQTDSRTIIDVSGAERLLGKGDMLF
                     VSSKYPRPLRLQAPFIEDGKSLEIIEYMGYSPCSVSSAFSSGFS"
     gene            complement(2364..3746)
                     /locus_tag="PK849_05620"
     CDS             complement(2364..3746)
                     /locus_tag="PK849_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPE65639.1"
                     /translation="MSINAKLGDILRAVTRTGADFADMYFEIAASHSYAFEDGSFEEI
                     SSSSSEGVGARILLGEATSLAYAPGVALETGASCLHEAAEHSGLSLPCRRMPAIRVME
                     REPPFPTPEYAFFYDLDKRIKASSMWIRQVSMNLDTAFKSFSVFNSDGSIAGDRRLYT
                     IFSVEVVVEKNGILQTGYESEAFSVDSGEFFRTVSPFKVAQRALSRALLMLDAPQCPA
                     GAMTVLLSGEAGGTMIHEACGHGFEADIIQKDYSVYRDKIGKQAASSLITLVDDGSIP
                     GYLGSGSFDDEGTPCKRNVLIEKGVVRSYISDIASSRRGALPLTGNGRRSSYRSVPQP
                     RMTNTYIEPGDSSPVEMLHSMNRGLLVKKMGGGEVDPTSGDFVFHVTEGYLVEGGRVL
                     HPVRGAVLTGNGPDVLMNIEAVGNDLHFLPGMCGKSGQSVPVTDGQPSLLVGNLTVGG
                     ANTCDDAIFE"
     gene            complement(3743..4027)
                     /locus_tag="PK849_05625"
     CDS             complement(3743..4027)
                     /locus_tag="PK849_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65640.1"
                     /translation="MSWIVSEYDGLGFVKTENAAVEKYMGNAVGSSSKGREAYVSLFF
                     PEKKRDDVLELLGVLKNEGLEIALLFELPPSPYTSKDSAKERTLEDFDTL"
     gene            complement(4108..5124)
                     /gene="mltG"
                     /locus_tag="PK849_05630"
     CDS             complement(4108..5124)
                     /gene="mltG"
                     /locus_tag="PK849_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPE65641.1"
                     /translation="MKKILCFFLAAAGLFMLIRTTAFSPERWPFPLPSGSGELLPVMV
                     QTGMNARQAADEFVAAGIVEKGRAASFARWMVRFSIDRRLRPGVYHVRKGSPWEVAMQ
                     MKTAEPGFLSLTLVPGTDIYSFGTLFPDKSLSGESLEKLLADESLYPSEMRNLLPETT
                     EGRLAFLLPETYHLAFLNERELVSAAAALWWRRIGSSIPPEQRSDEAFEELAILASLV
                     EREGFRDDERPRIAGVLSNRMEKGMQLQIDATVVYAWRRKGRDITRVLFKDLEIDSPY
                     NTYKIQGLPPAPICTSSEISWKAVLNPEQHDFLYYVAQSDGYHLFAETFSEHQKNIVK
                     VRSK"
     gene            complement(5150..>5981)
                     /locus_tag="PK849_05635"
     CDS             complement(5150..>5981)
                     /locus_tag="PK849_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE65642.1"
                     /translation="ASFAVDPIEKKPLYHWKPGTSVLSLGSVGCSMHCPFCQNWQLAE
                     CSASVSLQRIAPEDPARLAMQHGTPSVAFTYNEPLTWFEFLKDSCRLLREEKIPVVLV
                     SNGMINAAPLEEIVPYIAAANIDLKAFTPDAYRVMGGELDAVKRTIRTLSAAGTHVEV
                     TFLLVPGINDDAFSFAEMASWLASLHPVPVLHISRYFPNREWRVPATPLERIKEFERI
                     AHERLPWVYPGNIDGESCTRCLECGRVLLRRRRYTLLEKNIDEKGRCAFCESPTPVTL
                     "
BASE COUNT         1499 a         1633 c         1529 g         1320 t
ORIGIN      
        1 aagagaagcc agaagaaaaa gctgaagaaa cggaacaagg agaataaccc atgtactcta
       61 tgatctcaag gctcttgccg tcttcgatga agggagcctg aagacggagg gggcgaggat
      121 actttgaact gacgaaaagc atgtcgccct tccccaggag acgctccgcc ccggatacgt
      181 cgataatagt acgcgaatcc gtctgcgacg gaagggtaaa tgctactctc gccggaatgt
      241 tcgctttgat cagccccgtg atgacattca cggacggccg ctgagtggca aggacaaggt
      301 gaatacccgt cgccctggcc atctgtgcga ggcggcaaat ataatcttca acatccttct
      361 gcgctgtgaa catgaggtcg gccagctcgt caaccacgat gacgatatgc ggcaacttcg
      421 cttttggcag aacattctgg ttgtacgact tcagattgcg gacccttgcc cgggcgaaaa
      481 cttcataccg gtgctccatc tcccgcacag cccatgcaag cgcctggacg gcttttttgg
      541 gagagaccac cggtttggca agcacatgag gaagatgttc gtagatgctc agctctaccc
      601 gctttggatc aatcaggatc agcctgagct cttccggaga gcggtgcgtg cagagcccgg
      661 ttatacagct tgtaacgaag acgcttttcc ccgaccctgt ggttccggcg acaagaagat
      721 gcggcaagtc ctccagcccc acaaccaacg gacgggagtc gactcgaacg cccatagcaa
      781 gaggaaggtc attttccgag tccagaaaaa ctctcgattc aagtatggta cgaagagcaa
      841 tcccctttct tttcgggttg ggaatctcca cgccaacata cggttttccg gggatggggg
      901 cctctacacg gagcgaaggc acggtgagcg caacagcaag atcgttgctc aaaccggcaa
      961 ccttgctcac ctttattccc ggagcaagtt gtagttggaa ctggataacc gtcggaccga
     1021 tgacgatatc cgccatctcc gcacttacgc caaaatccgc gagcgtggtg ataatcgacg
     1081 aagcttgttc tcgaacggtt tcgttatcat ccgatgtctc catctcctgc ctgggaccga
     1141 aaatttccgg cggaggaggg aatgtgccgc tccggacacg accttcttct tccaccacat
     1201 gaaactcaat atcgggcaga gcgtgcggat ctgtgagaac atcttcgctg tcttgctcaa
     1261 ttttcgcgac gactgcatcg tcctccggaa gctccgtttg cagtccttgt cctgatatgt
     1321 tctcctccgg tcgtctcagg aggaagacat cctcctccat ctcaatatct gtgtcggtgt
     1381 cggtatcgtc cacttcgctc atttcttccc ggtctgaatg agtttcgaaa acttcaggaa
     1441 gcgcagcatc gggctgctcg gggagggctt cgctcctcgc ttcgtgtgga gtgagcgcaa
     1501 ctatcgagtt ttcagtaaga agaggctcat cctcagggag cgtttttctt ttccgcttga
     1561 aaaaaccaaa aagcgccccg ttcttttctt cttcgaatcc ttcggtgtcc tcctgaggaa
     1621 gccgctcttc tgaaagctgc gtttcttcta ttcgctgcag cacatccgca ggaataccgt
     1681 gagagggggg acgcaccaga acaaaacggg agggaagccc ggattcgttt tccgtttccg
     1741 tcttctcaac gggggtttct ttttggtgta catcttcagt atcaatgatg ccgcgtctcg
     1801 gtaacgaaag agaacgaaga gcattgatca tctttgaaat cctggatatt acagaagccg
     1861 gatgaataaa gccatagagg gttgcagaaa gaataagcaa cgccacggca aggagcgttg
     1921 ttccaagggc ccctatctcc ctggtaagaa agaccgccag accttgacct agatacccgg
     1981 gagtaaataa cgaaaaacct tgaaaaatac gggttgtctg aaagagcccg aacagaaggg
     2041 cagtacataa aaaaagaaga aacgtaccca gtgcttgcga gagaaaccct tttgcgcctg
     2101 tctttaaaaa ataggcaatg catagatacc ccccgaaaaa aacaggaatg agtatagcgc
     2161 cgccccagtt tcgcagtaga gaaaaacgga tcgcatcccc ccaaacgcct gttctgaggg
     2221 aaaagatcga agcgagcaca tagagaagaa aggcgaagat caaaaaagtg agcgtttcta
     2281 tccaaagcga cagtacgctg gagttttctc tcttgcctct tcctctggaa ctcgcagacg
     2341 ttttgacgaa cgtcttcttt tttttactcg aaaatagcgt catcacacgt gtttgcccct
     2401 ccgacggtga gatttccaac gagcagggat ggctgtccat cggtaacagg tacgctctgc
     2461 cctgactttc cgcacatccc tggcaggaag tgaagatcat tccccactgc ctcgatgttc
     2521 ataaggacat ccgggccgtt tccagtaagc accgctccac gtacagggtg aagaactctt
     2581 cctccctcga cgagataccc ttctgtaaca tgaaagacaa aatctcctga tgtaggatcc
     2641 acttctccgc cgcccatttt cttgacgaga agacctctgt tcatagaatg cagcatctcc
     2701 acaggcgagg aatctcccgg ttctatatac gtgtttgtca tccgcggctg cggaacggag
     2761 cgataggaac tccgcctgcc gtttccagtc aggggaaggg cgcctcgtct cgacgaggcg
     2821 atgtcgctga tatagctccg gacaaccccc ttttcgataa gaacgtttcg tttgcaggga
     2881 gtgccttcat cgtcaaagga accgcttccg agataccccg gaatagaacc gtcgtcgaca
     2941 agcgtgatca acgagctcgc ggcctgtttg cctatcttgt cccggtaaac ggaatagtcc
     3001 ttctgaatga tgtccgcttc gaacccgtgc ccgcaggctt catggatcat agtgcctcct
     3061 gcttctcccg agagcagcac cgtcattgcg cctgcagggc actgaggggc gtcgagcata
     3121 aggagagcac gagagagcgc cctctgcgcc actttgaacg gagaaactgt cctgaagaac
     3181 tcgcctgagt ccactgagaa ggcttcagat tcatacccgg tttgcagtat gccgtttttt
     3241 tcaacgacaa cctctacgct gaaaatcgta taaagacgcc ggtctccggc gatagagcca
     3301 tcgctgttaa aaacgctgaa actcttaaac gccgtgtcga gattcatgga tacctgccgt
     3361 atccacatag agctcgcctt tatacgttta tccagatcat aaaaaaaggc atactccgga
     3421 gtaggaaagg gcggctccct ctccatgacg cgaatcgcag gcatccggcg acaaggaagg
     3481 gaaagaccgc tgtgttccgc cgcttcatga aggcaggaag cgcccgtttc cagggcgact
     3541 cccggcgcgt acgcaagaga agtcgcttcg cccagaagaa tccgtgcgcc aactccttcg
     3601 gaagaagaag acgaaatctc ttcaaagctg ccgtcctcaa atgcatacga gtgcgacgcc
     3661 gctatctcaa aatacatatc ggcaaaatcg gcgccggtgc gcgtcacggc ccgcaatata
     3721 tctcctagtt ttgcgttgat gctcatagcg tatcgaaatc ctccaatgta cgttctttcg
     3781 ccgaatcctt cgaggtatat ggagaaggcg gcaactcgaa aagaagagct atctccaaac
     3841 cttcattttt aaggactcct agtaattcta acacgtcatc ccgtttcttc tccggaaaaa
     3901 agagagaaac ataggcctct ctccccttgg aggagcttcc gacagcattc cccatatatt
     3961 tttcaaccgc agcgttttct gtcttgacga atccaaggcc atcgtattcg gatacgatcc
     4021 agctcaagag cacaatctgc gcagcaggca gcccaagccc gaaacacccc atatcaggag
     4081 cgaaggagac agacggggga ggaaacatca cttggatcga acctttacga tgtttttctg
     4141 gtgctctgaa aaagtttcgg caaacaggtg atagccgtcc gactgggcaa cataatagag
     4201 aaaatcatgc tgttcaggat tgagaacagc cttccaggag atctcggagg aggtgcatat
     4261 cggtgcagga ggaagacctt gtattttata cgtattatag ggagagtcta tttccaggtc
     4321 cttaaagagc acgcgcgtta tatcccgtcc ctttcttctc catgcataga cgactgttgc
     4381 gtcgatttgg agctgcatcc ccttctccat tctattggaa agtacgccgg cgatgcgcgg
     4441 acgttcgtcg tcccggaatc cttcacgttc tacaagggaa gcgaggatcg ccaattcctc
     4501 aaaagcctcg tcgctcctct gctccggtgg tattgaggaa cctattcgtc tccaccagag
     4561 cgcggcagcc gcagagacca gctctctttc attcaagaaa gcgaggtgat acgtttcggg
     4621 aaggagaaag gcgagccggc cttcagttgt ttcggggagc aaattgcgca tttccgatgg
     4681 atacaggctt tcatccgcca acagtttttc cagcgactct ccggaaagtg acttgtccgg
     4741 gaagagcgtg ccgaaagaat agatatcggt accgggtaca agcgtcaaag aaagaaagcc
     4801 cggctctgca gttttcattt gcatcgctac ttcccaagga gagcccttcc gaacatggta
     4861 cactccgggc cgcagacgac ggtcgatgga aaagcgcacc atccagcgag caaatgaggc
     4921 ggcccggccc ttctcaacaa tgcccgccgc cacaaactca tccgctgcct gccgcgcgtt
     4981 catccccgtc tggaccatca ccggaagcag ttcgccgctt ccgctcggga gaggaaacgg
     5041 ccaccgttct ggagagaacg cagtggtacg gatcaacatg aagagtccgg cagcagcgag
     5101 gaaaaaacac agtatttttt tcatagaaat gcattcctcc tagagacagc taaagagtga
     5161 ccggcgtcgg cgactcgcag aaagcgcacc gccccttttc gtcaatattc ttttcaagaa
     5221 gagtatagcg gcgtcttcga agcaaaactc ggccgcactc caaacaacgc gtacaactct
     5281 ctccgtcgat atttccggga tacacccacg gaagtcgttc atgtgcaata cgctcgaact
     5341 ccttgatacg ttccaaagga gtcgcgggaa cccgccattc tcgattcggg aagtagcggg
     5401 aaatatggag cacaggaacc gggtgaagag aagcgagcca agacgccatc tccgcaaagg
     5461 agaaagcgtc atcgttgatt ccaggcacaa ggagaaaggt gacttctacg tgcgttcccg
     5521 cggccgaaag agtcctgatc gtccttttta ccgcgtcgag ctctcccccc ataacacggt
     5581 acgcgtccgg tgtaaaggct ttcaagtcaa tgtttgcggc ggcaatatac gggacgatct
     5641 cctccaatgg agctgcgttg atcattccgt tgcttaccag tacgacgggg atcttttcct
     5701 ctcgaaggag acggcaggaa tccttgagaa actcgaacca ggtcaaaggc tcattatagg
     5761 tgaacgcgac ggagggcgtt ccgtgctgca tcgcgaggcg cgcgggatcc tcaggggcta
     5821 tcctctgcaa tgaaaccgac gctgaacact ccgcaagctg ccagttctga cagaaggggc
     5881 agtgcatcga acaccctacg gatcccagag acagtaccga agttcccggc ttccagtgat
     5941 agagaggttt cttttcgatg ggatccactg cgaaagaggc c
//
LOCUS       DAOPKU010000137         5965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14308_length_5965_cov_5.079019, whole genome shotgun sequence.
ACCESSION   DAOPKU010000137 DAOPKU010000000
VERSION     DAOPKU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14308_length_5965_cov_5.079019"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK849_05640"
     CDS             complement(<1..345)
                     /locus_tag="PK849_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPE65643.1"
                     /translation="MKPFSLVPVFKDYLWGGTKLKTRFNKRTDVSPLAESWELAAHPD
                     GDCRIANGSFAGTPLSEFLRKFPESLGSGRVCGGGLPVLIKLIDAREPLSVQVHPDDV
                     YAMRTEGGAGKTE"
     gene            complement(391..1038)
                     /locus_tag="PK849_05645"
     CDS             complement(391..1038)
                     /locus_tag="PK849_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPE65644.1"
                     /translation="MKERTSKIVAELFAPGDALEGIRESALQAGELLRDSFAGGGKLL
                     VCGNGGSWADGEHIVGELMKGFLLKRPLPDDAKRMLTLLWGDEGRAIGEALQGALPAI
                     SLGSHGALMTAFANDADGRFAFAQQAMGYARPGDVALGISTSGNAENVRFALMAAKSR
                     GASTVALTGADGGLLAKYADCCIRVPDRRTFRVQEHHLRIYHFLCAFVESELFDE"
     gene            complement(1035..2027)
                     /locus_tag="PK849_05650"
     CDS             complement(1035..2027)
                     /locus_tag="PK849_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPE65645.1"
                     /translation="MYAIGVDIGGTKCAVCLGKGRGDTLRIVHKCEPRKTAEYSPAEM
                     LDALLADVRECRSRIPARETVIGTGISCGNPLDSRAGVILSPPNLPGWDRIEVVDWFR
                     SGTGLPAWLCNDANAGALAEWRYGAGRGCEHLIFLTFGTGFGAGLILNGRLYAGASDA
                     AGETGHVRAAAHGPVGYGKIGSYEAFCSGGGIAQIARTYALRDIQNGKPSALCPDVAG
                     LESLTAESTARAAREGNPTAKEVFELSGAEFGRALSMLIDLFNPQRIVVGGIFSRCRD
                     LIWPAAERVIAEEALPDSRKACTVLPCELSESIGDMAALSVAVYYWEHGGEGKR"
     gene            complement(2132..3040)
                     /locus_tag="PK849_05655"
     CDS             complement(2132..3040)
                     /locus_tag="PK849_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPE65646.1"
                     /translation="MKKAVAVFLGCVLLAGLFVGGAAAAEKHKVYLITMDQMDQHWVT
                     VNAGAEKAVAELGTIDYKWLAPDVKDDAKQIEAVNNAVAGGAEVLLVAANGPTAISAA
                     LKEASEAGVKIVYVDSPADFPAIQTLATDNEAAGVTAGKEMLKALEAAGVAEGKIGIV
                     NVNAATASTVAREKGFRAAFEGTKFELLETQYCDGDAARAKDIASNYITQGCVGIFGA
                     NEGSTVGTGNAIQEAGGKVVGVGFDKSNMILSLIKNGFLVATMAQNPDVMGYEGVKTA
                     AKALAGEPTGAEYVDTGVSVLTKNDL"
     gene            complement(3154..4176)
                     /locus_tag="PK849_05660"
     CDS             complement(3154..4176)
                     /locus_tag="PK849_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE65647.1"
                     /translation="MDDSKSPSRLGRLFAARGMGQVVTAAFGLVILCVVFGILNPIFF
                     SGRNVGNLLRQIAPILLIGIGQSYVLITGNIDLSIGSVVGMSCMVSATLMTMGMNPWV
                     AVAVTLICCLMVGVLNGCLVAKFNLPPFIATLGTMTIARGIAQIVNNNYNTDSIGPAA
                     EGFRNFFYYGRLGGLFNTIWISLALWLVFNFILSRTRLGRHIYATGSNKEAARLSGVD
                     VVASTTAAYMVSAVCSCVVGLIVCATSGMGTMDAGNMYELYAVAASVIGGVSTLGGQG
                     LLLGTVMGASIWGVLQNGLQFAGAPVAIRNIVIGVIVVVSVLLDVVVRSEKRRRKRER
                     KEEAKG"
     gene            complement(4208..5725)
                     /locus_tag="PK849_05665"
     CDS             complement(4208..5725)
                     /locus_tag="PK849_05665"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPE65648.1"
                     /translation="MSEYIVELEGVGKSFAGIRALDDVSLHLKSGEVLALLGENGAGK
                     STLMKILGGIYTRDTGIIRVFGEEIDEMTPKRAKELGIAVIHQELNMCTHLTVAENIF
                     LGREILRSGMLDDREMNIRAAEVLSRLNIDIAPDTVVGSLPISKQQMVEIAKALSTNA
                     RILVMDEPTSALTSKEINDLFHIIRSLRDQGCGIIYISHRLEELQHIVDRVTILRDGK
                     YIATTRFCDTTMAEIISNMVGREIKEKFPRVETPLGRTILRVKGLNAGRQVRNVSFEL
                     REGEIVGVAGLMGAGRTEMTRALFGADPKDGGEILLDERPLRIEKPMDAIEAGIVLVP
                     EDRKKDGLCTKLSIRENVALPNLDILKGALSTVNSRGEREMVERTVKNLSIRLSNAEQ
                     NADSLSGGNQQKLVVGKWLARDFRVVLFDEPTRGIDVAAKAEIYHLMNDLKTRGLGVL
                     FISSEMPEILGIADRIIVMCDGRVTGEFPAGTATQEKILECATRFEDKLGLAAGD"
BASE COUNT         1056 a         2032 c         1705 g         1172 t
ORIGIN      
        1 ctccgtcttc cccgcgcccc cctccgtgcg catcgcatac acgtcgtccg gatgcacctg
       61 taccgaaaga ggttccctgg cgtcgatcag cttgatcagt acggggagcc cccctccgca
      121 gacgcggccg cttcccaggg attcagggaa cttccgcaga aactccgaca gcggggttcc
      181 ggcgaaggag ccgttcgcta tgcggcagtc tccgtccgga tgcgcggcaa gctcccagct
      241 ctcggcgaga ggggatacgt cggtcctctt gttgaaccgc gtcttaagtt tcgtcccgcc
      301 ccagagatag tccttgaaga cgggtacgag tgaaaacggc ttcatgcaac ttcccccctt
      361 tcccctgtga aagaacgccg cccggcgttg ctattcatcg aacagctccg actcgacgaa
      421 cgcacagagg aaatgataga tcctcaagtg gtgctcctgg acgcggaacg tccttctgtc
      481 gggaacgcgg atgcagcagt ccgcatactt cgccagcagc cccccgtccg caccggtcag
      541 cgcgacggtc gacgcgccgc ggctcttcgc cgccatcagg gcgaaacgaa cgttctccgc
      601 attccccgac gtgctgatac ccagcgcgac atctccgggg cgcgcgtacc ccatcgcctg
      661 ctgcgcgaag gcgaaccgtc cgtcggcgtc gttggcgaag gcggtcatca gcgctccgtg
      721 gctcccgaga gatatcgccg gaagcgctcc ctgcagcgcc tcaccgatcg cgcgcccttc
      781 gtccccccag aggagagtta acatgcgctt cgcatcgtcc gggagggggc gtttcagaag
      841 gaatcctttc atcagctcgc cgacgatgtg ctccccgtcc gcccagctcc cgccgttgcc
      901 gcagaccagc agcttccccc cgccggcaaa agagtctctc agcagctctc ccgcctgcag
      961 cgcgctctcg cgaatgcctt ccagcgcgtc gcccggcgcg aacagctccg cgacgatttt
     1021 cgatgtacgc tctttcatcg ttttccctcg cctccgtgtt cccaataata caccgcgacg
     1081 gacagggccg ccatatcccc gatggattcc gacagctcgc agggaagcac cgtgcaggct
     1141 ttccgggaat cgggaagcgc ctcctccgcg atcacgcgct cggcggcggg ccagatgaga
     1201 tcgcggcagc gtgaaaagat gcccccgacg acgatacgct gaggattgaa gaggtcgatg
     1261 agcatcgaga gcgcgcgtcc gaactccgcc ccggagagct cgaaaacctc tttcgccgtt
     1321 ggatttcctt cgcgcgcggc gcgggctgtc gactccgcgg tcagcgattc caggccggcg
     1381 acatccgggc acaacgcgga cggcttcccg ttttggatat cgcgcagggc ataggtccgg
     1441 gcaatctggg caataccgcc tccgctgcag aacgcctcgt acgacccgat tttcccgtac
     1501 ccgacgggac cgtgcgccgc agcgcggacg tgtcccgtct cccctgcggc gtcgctcgct
     1561 ccggcgtaga ggcggccgtt gaggatcagc cccgcgccga acccggtgcc gaaggtcagg
     1621 aagatcaggt gttcgcaccc cctcccggcg ccgtaccgcc actccgccag cgcacccgcg
     1681 ttggcgtcgt tgcagagcca cgccggaagc ccggtcccgc tccggaacca gtcgacaact
     1741 tctatgcggt cccaaccggg caggttcggg ggggacaata tcacccccgc ccggctgtcg
     1801 aggggattgc cgcagctgat tcctgtgccg atgaccgttt ccctcgccgg aatcctcgat
     1861 ctgcactccc gaacgtcagc gagcagtgcg tcgagcatct ccgcaggcga gtactccgcg
     1921 gtcttcctgg gttcgcactt gtggacgatg cgcaaagtat ctcctctgcc cttcccgagg
     1981 catacggcgc acttcgttcc tcctatatcg acgcctatcg cgtacattcc catccctccg
     2041 agctcacggg agctcccgca agggcgctcc tactacagaa ggcccccgcc ctgagggacg
     2101 ggggccttct tcatactccg gtacgctcct tctacaggtc gttcttcgtc agaacggaga
     2161 cgccggtgtc cacatactcg gctcccgtgg gctctcctgc aagagctttg gccgcggtct
     2221 tcacgccttc gtaccccatg acgtccgggt tctgcgccat ggtggcgacg aggaagccgt
     2281 tcttgatcag gctgaggatc atgttcgact tgtcgaagcc cacgccgacc actttgccgc
     2341 cggcctcctg aatggcgttg cctgttccga cggtcgagcc ctcgttggct ccgaagatgc
     2401 cgacgcaccc ctgggtgatg tagttggagg cgatgtcctt cgcccgtgcg gcgtcgccgt
     2461 cgcagtactg cgtctcgagc agctcgaact tcgtcccttc gaacgccgcg cggaagccct
     2521 tctcgcgggc gaccgtcgac gcggtggccg cgttgacgtt gacgatgccg atcttgccct
     2581 cggcaactcc ggccgcttca agcgccttca gcatctcctt gcccgcggtg actccggcgg
     2641 cctcgttgtc cgtcgccagc gtctggattg cggggaagtc cgcaggagag tcgacgtaca
     2701 cgatcttgac gcccgcctcg gaagcctctt tcagcgccgc gctgatcgcc gtcggtccgt
     2761 tggccgccac gaggagcact tcggctccgc cggcaacggc gttgttgacc gcttcaatct
     2821 gtttggcgtc gtccttcaca tcgggagcga gccacttgta gtcgatagtg ccgagctccg
     2881 ccaccgcttt ttcagctccg gcgttcaccg tcacccagtg ctggtccatc tggtccatgg
     2941 tgatgagata caccttgtgc ttctccgccg ccgccgcgcc gccgacaaaa agtccggcca
     3001 gcaaaacgca ccccagaaac accgctactg cttttttcat tcgtccgttc ccccttgaag
     3061 atatgatgtg aaaccgttgt atcgaaagaa gcggagagtt ccgtctctcc ccggaagcgc
     3121 tgcgtcgaac aggcctcgct ccggggaaga gcatcagccc ttcgcctctt ctttccgttc
     3181 cctttttctg cgccgcttct cgcttctgac gacgacgtcg agaagaaccg agacgaccac
     3241 gatcacgccg atgacgatgt tgcgtatcgc cacgggggcc cctgcgaact gaagcccgtt
     3301 ctgcagcacg ccccagatcg aggcgcccat caccgttcca agcagcagcc cctgtcctcc
     3361 gagcgtgctc acgccgccga tgaccgaggc cgccacagcg tacagctcgt acatattgcc
     3421 cgcgtccatc gtccccatgc cgctggtggc gcagacgatc agcccgacga cgcaggaaca
     3481 gacggcgctg accatgtacg cggccgtcgt cgacgcaacg acgtcgacgc ccgagagacg
     3541 cgccgcctcc ttgttgcttc cggtggcgta gatatgccgc ccgagccgcg ttcgtgatag
     3601 gatgaagttg aagaccagcc agagcgcgag cgagatccag atggtgttga aaaggccgcc
     3661 cagcctgccg tagtagaaga agttccggaa cccctcggcg gcaggcccta tggagtcggt
     3721 gttgtagttg ttgttcacga tctgcgcgat gccgcgcgca atggtcatcg tccccagcgt
     3781 cgcgatgaag ggggggagat tgaacttcgc caccaggcat ccgttgagga cgcccaccat
     3841 caggcagcag ataagcgtga cggccacggc gacccacgga ttcatcccca tcgtcatcag
     3901 cgtcgccgag accatgcagc tcatccccac cacggaaccg atggagaggt cgatattgcc
     3961 ggtgatgagc acataggact ggccgatgcc gatcaaaaga atcggcgcta tctggcgcag
     4021 cagattcccg acgttgcgcc cggagaaaaa gatcgggttc agaatgccga acaccacgca
     4081 gaggatcacc agaccgaacg cggcggtcac aacctgtccc atgcctctcg ccgcgaaaag
     4141 acgcccgagg cgggaggggc ttttcgaatc gtccatgctc acagtcatcg ctccccgtgt
     4201 agaataatca atccccggcc gcaaggccga gcttgtcctc gaagcgcgtg gcgcattcga
     4261 ggatcttctc ctgcgtcgca gtcccggcgg ggaactcccc cgtcacgcgg ccgtcgcaca
     4321 ttacgataat cctgtccgcg ataccgagga tctccggcat ctccgaggag atgaagagca
     4381 cgccgagccc tcttgttttc aagtcgttca taagatggta tatctccgcc ttcgccgcca
     4441 cgtcgatccc cctggtcggc tcgtcgaaga ggaccacgcg aaagtcccgg gcaagccact
     4501 tgccgaccac cagcttctgc tggttgccgc cggagaggct gtcggcgttc tgctccgcgt
     4561 tcgagagcct gatcgagagg ttcttcaccg tccgctccac catctcccgc tcgcctctgc
     4621 tgttcaccgt tgacaacgct cccttgagga tatcgaggtt cggaagggcg acattctcgc
     4681 ggatgctcag cttggtgcag agcccgtcct tttttctgtc ctccggcacg aggacgatac
     4741 cggcctcgat cgcgtccatc ggcttttcaa ttcgcagagg ccgttcgtcg agcaaaatct
     4801 ccccgccgtc cttcggatcc gctccgaaga gcgcgcgggt catctcggtc ctgcctgcgc
     4861 ccatgagtcc ggcgacgccg acgatctctc cttcgcgcag ctcgaagctc acgttccgca
     4921 cctgacgccc cgcgttcagc cccttcaccc ggagtatcgt ccgcccgagc ggtgtctcca
     4981 ccctcgggaa cttctctttg atttcgcgtc ccaccatgtt cgagatgatc tccgccatgg
     5041 tggtatcgca gaacctggtc gtcgcgatgt acttgccgtc gcggaggatg gtcacgcggt
     5101 ccacgatatg ctgcagctct tcaagccggt gcgagatata gatgatgccg cacccctggt
     5161 cgcgcagcga acggatgata tggaacagat cgttgatttc cttggaagtg agcgcggagg
     5221 tgggctcgtc catgacgagg atccgcgcgt tcgtggagag cgccttggcg atctccacca
     5281 tctgctgttt cgagatggga agggatccca ccactgtatc gggagcaata tcgatgttca
     5341 gccgggagag gacctcggcg gcgcggatgt tcatctccct gtcgtcgagc ataccggaac
     5401 ggaggatctc cctgccgaga aagatgttct ccgcgacggt gaggtgggtg cacatgttca
     5461 gctcctgatg gatgacggcg atgcccagct ctttcgcccg cttcggcgtc atctcgtcga
     5521 tctcctcccc gaagacccgg atgatcccgg tgtcgcgggt atagattccg ccgaggatct
     5581 tcatcagcgt cgactttccg gcgccgttct cgcccagaag cgcgagcacc tccccgctct
     5641 tcaggtgcaa cgaaacgtcg tccagcgccc tgattccggc gaaggatttg ccgacgccct
     5701 caagctcgac tatgtattcc gacacgccgc cacctccgct cgcctcgcag atgcattcat
     5761 gatcggcgca ggcgttcttt tcctataccg gatgttgtgt agagaaacgc gtattcatcc
     5821 catttgacga actgccgctg tcgaaagagc aaatacatct ccctaaattt tcatccttca
     5881 tagtgtagca cacggaacag ccttccagca acgagcatcc tctcttgcga tggagggaaa
     5941 atatgttcgc gaagaagcat ccgtt
//
LOCUS       DAOPKU010000138         5936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14407_length_5936_cov_4.673525, whole genome shotgun sequence.
ACCESSION   DAOPKU010000138 DAOPKU010000000
VERSION     DAOPKU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14407_length_5936_cov_4.673525"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PK849_05670"
     CDS             complement(<1..1261)
                     /locus_tag="PK849_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPE65649.1"
                     /translation="MKRTVELTDLLRYHFLSAPTFSPDGTKIAFLLHKADYENNAYSS
                     NIWMHDLLTGSLRQLTASGKEKFFAWKHDNRTLVFASGRNDAPKTETVFYTLDICGGE
                     AVPLRTVKRALSSFTVLSDGTFLALGVFEQAFDNPYDAEMLFFDQVPFCSNGKGFTGQ
                     KKKSLCLYTPEGEEKRLTPEDMDVENYVVSPDGEKLLAWGPVFKDVKGQFSALLQIDR
                     KSGATTTLLPGTDFACKWAGWLDGKAVVTGTDFKKYGVNENVKFFFVENGETACITPE
                     LDKGLRNSVGSDCRYGSTDLNLAFFTDGPAAWFVSTDNYRSHLHAVTASGAMEQITKE
                     LFSVDDWRVSGGKAAVVGMKGLQLQELYLVDREREIQLTHFNDWLLEECELSAPEHVT
                     FDNGTGEQLDGWYMKPIGFEEGKKYPAI"
     gene            complement(1294..1566)
                     /locus_tag="PK849_05675"
     CDS             complement(1294..1566)
                     /locus_tag="PK849_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPE65650.1"
                     /translation="MIKVLFFATIRDYTNEKETTAEGASSVEGLLRLLCARYGPEFET
                     EVFDGGKISDRVIVLVNGKHIAHTGGPLTPVKDGDTIAVFPIIGGG"
     gene            complement(1571..3367)
                     /locus_tag="PK849_05680"
     CDS             complement(1571..3367)
                     /locus_tag="PK849_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPE65651.1"
                     /translation="MDKFGSIVRINLTTGSIKKESLCEKTMKEFIGGRGYATKVLYDE
                     LPKGTDPLSPENKLIFANGPLTGKGAPASGRYMVVTKSPLNNYIASSNSGGFWGAELA
                     RAGWFMIILEGKAQKPSYIWINDDKVEIRDASAFWGLDSHETTDKLLAAVGDDKAKVA
                     CIGPAGERLSKLASVMNDKNRAAGRSGVGAVMGSKNLKAIVVKGSSRPHVENQDEMKS
                     VLATAMEKLKTNPVTSQGLPTYGTAVLVNIINQIGGYPYKNWQGAHMPEADSQSGETL
                     ARDFLVKRYSCYACPIGCGRVTKVGDREGEGPEYESIFSFGVCCGVEPLEPIIEANYL
                     CNEYGLDTISAGVTIAAAMELYEKGYIKKEELEGGPELKFGNAEAVTYWTKKMGLVEG
                     FGKKLAMGSYALCEMYGHPEFSMTAKKLEMPAYDGRAVQGIGLNYATSNRGGCHVRGY
                     TTSPEVLGLPQKLDPAEYQSKPTWVKIFQDLTAAIDSSGMCLFTSFALGADDYAAFLK
                     AATGFEYDGAEALKAGERIWNIERLFNLREGMDTAEDTLPARLLNEPIPEGPAKGMVS
                     HLPEMLPEYYKLRGWDSKGIPTTGKLEELGLQ"
     gene            3671..4270
                     /locus_tag="PK849_05685"
     CDS             3671..4270
                     /locus_tag="PK849_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HPE65652.1"
                     /translation="MLSWFDFLPLRADRQGPWQVVSFLEVREAAEDEGLSPVEAEIRC
                     LQRGYVPSRYIRSMGTIGIDGQIRLLSSAVAVIGCGGLGGLVADLLARAGVGRLVLVD
                     GDVFDETNLNRQILSFEGNLGLSKSKVAAQRVTEINGVVRAEGRQCMFDEFTADGILE
                     GCDLAVDCLDNLSTRRILFSECRTRGIPVVSGAIGGFWG"
     gene            4283..4501
                     /locus_tag="PK849_05690"
     CDS             4283..4501
                     /locus_tag="PK849_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65653.1"
                     /translation="MPDEQNLAEFLSGDTDKGVETETGNPPFTPAVMAALECAQAVKI
                     LTGKGKLLTDQLLWMDLAEDEFTRLRLS"
     gene            4523..5320
                     /locus_tag="PK849_05695"
     CDS             4523..5320
                     /locus_tag="PK849_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPE65654.1"
                     /translation="MKLFQVDAFTDRLFAGNPAGVCLLEAEVPDAWMQSMAAEMNLSE
                     TAFLLGTPSGWSLRWFTPRVEVALCGHATLSTAHIMLQEGMLQQGHEAVFHTKSGPLS
                     ARAEDEWIVLDFPAKKVVPVAPPPGLIEALGLERDEYLFCGKNKDDYLLELPDEELVR
                     SLDPDFVALSDVEARGTMVTAPSSRCEFDFASRFFAPAVGVNEDPVTGSAHCALAPYW
                     GKKLGKTLMNAEQVSARGGVMLVEVQGERVLLRGKAVTVFRGEMTVS"
     gene            5430..>5936
                     /locus_tag="PK849_05700"
     CDS             5430..>5936
                     /locus_tag="PK849_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE65655.1"
                     /translation="MLDALRVTVLAEDSVPYESPLLAQHGVSFWLEAEHNGNVQRVLV
                     DVGQNPDALLYNIEQLKIPLGETDAVVLTHCHYDHTQGLSKILKAIGRRDVPVIAHPA
                     LFRPNFITAPFFRHVGVMQGDEPLDIQAAGGSLFLFSEQLQLMPGLETTGEVPRTTDF
                     EEVGIALCT"
BASE COUNT         1047 a         1702 c         1850 g         1337 t
ORIGIN      
        1 ggatggcggg gtacttcttt ccctcctcga aaccgatcgg cttcatgtac cacccgtcga
       61 gctgttcgcc cgttccgttg tcgaacgtca cgtgttccgg cgccgacagt tcgcactcct
      121 ccagcagcca gtcgttgaag tgggtgagct gaatctcgcg ttccctgtcc acgaggtaga
      181 gctcctgaag ctggagcccc ttcatcccca cgacggccgc tttgccgccg gaaaccctcc
      241 agtcgtccac gctgaagagc tccttcgtga tctgctccat cgccccggag gctgtgactg
      301 cgtgcagatg cgagcggtag ttgtccgtgg agacgaacca ggccgccgga ccgtccgtga
      361 agaaggcgag gttcaggtcg gtcgagccgt agcggcagtc gcttcccaca gagttccgga
      421 gccccttgtc aagttcgggg gtgatgcagg ccgtttctcc gttctcaacg aagaaaaact
      481 tcacgttctc gttcactccg tacttcttga agtcggttcc ggtgacgacc gcctttccat
      541 cgagccaccc agcccacttg cacgcgaagt cggttccggg aaggagcgtg gttgtagcgc
      601 cgctcttccg gtcgatctga agcagtgcgc tgaactggcc cttgacgtct ttgaagaccg
      661 gcccccaagc aagcagcttc tccccgtccg gagagacgac gtaattttcc acgtccatat
      721 cctcgggggt gaggcgtttc tcctcgccct ccggagtgta gaggcagagc gactttttct
      781 tctgccccgt gaaccccttt ccgttggagc agaaaggtac ctggtcgaag aagagcattt
      841 ccgcgtcgta agggttgtcg aacgcctgct cgaagacgcc gagcgcaagg aaagtgccgt
      901 ccgaaaggac ggtaaacgag gagagcgcgc gcttcacggt gcgaagcgga acggcctcgc
      961 ctccgcagat gtcgagcgtg tagaacaccg tctccgtctt gggggcgtcg ttgcgtccgc
     1021 tggcgaagac gagcgtcctg ttgtcgtgct tccacgcgaa aaacttctcc ttgccggagg
     1081 ctgtgagctg ccgaaggctt cccgtaagca ggtcgtgcat ccagatgttc gagctgtagg
     1141 cgttgttttc gtagtcggcc ttgtggagga gaaatgctat tttcgtgccg tcgggagaga
     1201 acgtcggtgc ggagagaaag tggtatcgaa gcagatcggt gagttctacg gttcttttca
     1261 attttctggt cccctttgcc tggaagtgtg cggctatcct ccgccgatga tagggaagac
     1321 ggcgatcgtg tcgccgtcct tgaccggggt gagcggtccc ccggtgtggg cgatgtgttt
     1381 gccgttcacc aggacgatca cccggtcgga aatttttccc ccatcgaaaa cctcggtttc
     1441 gaactcggga ccgtagcgcg cgcaaaggag acggaggagc ccctctactg aagaggctcc
     1501 ctccgccgtt gtctccttct cgttcgtgta gtcccggata gtcgcgaaga acagtacttt
     1561 tatcatgcgc ttattgtaac ccgagttcct ccagttttcc agtggtgggg atgcctttgg
     1621 agtcccagcc gcgaagcttg tagtattccg gaagcatttc cggcaggtgg ctcaccatgc
     1681 ccttggccgg gccttcaggg atcggttcgt tcagaaggcg cgcggggagc gtgtcctctg
     1741 ctgtatccat accctcgcga aggttgaaca gacgctcgat attccagatg cgctcgcctg
     1801 ccttcagggc ttcggcgccg tcgtattcga agccggtggc ggccttcagg aacgcggcgt
     1861 agtcgtcggc gcccagggcg aaggaggtga agaggcacat gccggaggag tcgatcgccg
     1921 cagtgagatc ctggaagatc ttgacccacg tcggctttga ctgatactcc gccgggtcga
     1981 gcttctgagg caggccgagc acttcgggcg aggtcgtgta gccgcggacg tggcagccgc
     2041 cgcggttgga ggtggcgtag ttcaggccga tcccctggac ggcgcgtccg tcgtacgcgg
     2101 gcatctcaag cttcttggcc gtcatggaga actcggggtg cccgtacatc tcgcagaggg
     2161 cgtaggaacc catggcgagc ttcttgccga atccctcgac gagtcccatc tttttcgtcc
     2221 agtaggtaac ggcttcggcg ttgccgaact tcagttcggg gccgccctcc agctcttcct
     2281 tcttgatgta ccccttctcg tacagctcca tggccgccgc gatggtcacg cccgcgctga
     2341 tcgtgtcgag gccgtactcg ttgcagagat agttcgcttc gatgatcggc tcgagaggct
     2401 ccacgccgca gcagacgccg aaggagaaga tgctctcgta ctcggggccc tcgccctcac
     2461 ggtcgccgac cttggtcacg cgtccgcagc cgatggggca tgcgtagcag gagtatctct
     2521 tgacgaggaa atccctggcg agcgtctcgc cggactgtga gtcggcttcg ggcatgtggg
     2581 cgccctgcca gttcttgtag gggtagccgc cgatctggtt gatgatgttc acgagcaccg
     2641 cggtgccgta ggtgggaagc ccctggcttg tgacggggtt ggtcttcagc ttctccatcg
     2701 cagtcgcgag gacggacttc atctcgtcct ggttctcgac gtgggggcgg ctgcttccct
     2761 tgacgacgat cgccttcagg ttcttcgagc ccatgacggc gccgacgccg cttcgtcccg
     2821 cagccctgtt cttgtcgttc atcacggagg cgagtttgga aaggcgctct ccggcggggc
     2881 ctatgcaggc aaccttcgcc ttgtcgtcgc cgacggccgc gaggagcttg tcggttgttt
     2941 catggctgtc gagcccccag aatgcggagg cgtcgcggat ttcaactttg tcgtcgttga
     3001 tccagatata ggaaggtttc tgcgccttgc cttcgaggat gatcatgaac catccggcgc
     3061 gcgccagttc cgctccccag aatccgccgg agttgctgct tgcgatatag ttattgagag
     3121 ggctcttcgt cacgaccatg taccttccgg acgcgggagc gcccttccct gtaagcggac
     3181 cgttggcgaa gatcagcttg ttctccgggg aaagcgggtc ggtacctttg gggagctcgt
     3241 cgtagagcac ctttgtcgcg taccctcgtc ctccgataaa ctccttcatc gttttctcgc
     3301 acagactttc ctttttgatg ctgccggtgg tgaggttgat acgcacaatc gatccgaact
     3361 tatccataag cagttccctc cttgtgtatt cgttcatctt gcaataccct tgttgccgat
     3421 acaaaaaaag aagaatgaca gcctgtaaag ctgttcatcc ttctttgcaa acacggccgg
     3481 cgggaagatc actcttccaa cccgatgccc ggcacatctc ggggagacgc ggcgggtcga
     3541 aggcatattc ggttggcttc cttggaacat ttattagttc actttatttt tataccataa
     3601 atggcgacct gtcaattctc tgcggggtat tttccttgta taatcattct gcgcatcggg
     3661 aggtgagtgt gtgttgagtt ggtttgattt tcttccttta agagcagacc ggcaggggcc
     3721 gtggcaggtt gtttcgttcc tggaggtgcg cgaagccgcg gaggatgaag gcctgtcgcc
     3781 ggtcgaagcc gaaattcgct gtctgcagcg cgggtacgtt ccttccagat acatcagaag
     3841 tatgggcact atcggcatcg acggccagat caggctgctg tcctccgccg tggcggtgat
     3901 aggctgcggg ggacttggcg gccttgtcgc cgatcttctt gcgcgggccg gagtgggacg
     3961 tctggtgctg gtcgacggcg acgtcttcga cgagacgaat ctgaaccgtc aaatactctc
     4021 cttcgagggg aaccttggac tctccaaatc aaaggttgcg gcgcagcgcg ttacggagat
     4081 caacggagtc gtccgcgccg agggtcggca gtgcatgttc gacgaattca ccgcggacgg
     4141 catactcgaa gggtgcgatc tggccgtgga ctgcctggat aacctgagca cgcggcgcat
     4201 tctcttctcc gagtgccgga ccaggggaat cccggtagtc tcgggggcca tcggcggctt
     4261 ctgggggtaa atcggcgtcg ccatgcctga cgaacagaac cttgcggaat tcctgtcggg
     4321 cgatacggac aagggggtcg agaccgaaac cggcaaccct ccctttactc ctgctgtgat
     4381 ggccgcgctt gaatgcgctc aggccgtgaa gatcctcact ggcaagggaa aactcctcac
     4441 ggaccagctg ttgtggatgg atctcgccga ggacgagttc acgcggcttc gcctgagctg
     4501 aaaaacgaaa gggggaaacg gcatgaaact ttttcaagtc gatgcgttca ccgatcgtct
     4561 cttcgcagga aatccggcgg gcgtctgtct gctcgaagct gaggttcctg atgcctggat
     4621 gcagagcatg gccgcggaga tgaacctctc cgagacggcg ttccttctgg gaaccccatc
     4681 cgggtggtcc ctccgatggt ttacgccgcg cgtcgaggtg gctttgtgcg gccacgcgac
     4741 gctctccacg gcgcatatta tgctccagga gggaatgctt cagcaggggc atgaggctgt
     4801 ctttcacacg aagagcggcc ctttgagcgc acgagccgag gatgaatgga tcgtgctcga
     4861 ctttccggcg aagaaggtcg ttcccgtcgc ccctcctccc gggctcatcg aggcgctcgg
     4921 gctggagcgc gacgaatacc tcttctgcgg gaagaacaag gacgactatc tccttgagct
     4981 ccccgacgag gagcttgtac gctcgctcga tcccgacttc gtcgcgctct ccgatgtgga
     5041 ggccagggga accatggtga cggcgccgtc gtcgcgttgc gagttcgact tcgcatcccg
     5101 cttcttcgcg cctgctgttg gcgtgaacga agacccggtg acgggctccg cgcactgtgc
     5161 gcttgccccc tactggggga aaaagctggg gaagacgctg atgaacgccg agcaggtctc
     5221 cgcgcgcgga ggcgtgatgc ttgtcgaggt gcaaggggag cgggtgcttc tgcgaggaaa
     5281 ggccgtaacg gtcttccgcg gagagatgac ggtttcctga acacacgggc gttcgcttat
     5341 cagatgaccg gtctctcttc ccgtttgaat ttgcggaaca gatggcgttg acgcggagaa
     5401 aacgctcttt tctttggaga tggtgcagaa tgttggatgc gctcagggta acggttcttg
     5461 ccgaggacag cgttccttac gaaagtccgc ttctcgctca gcacggggtt tcattctggc
     5521 tggaggcgga acacaacgga aatgtacagc gggttcttgt cgatgtggga cagaatccgg
     5581 atgcgctgct ctacaatatc gagcagctga agatccctct cggggagacc gacgcggttg
     5641 tactgactca ctgtcactac gatcacacgc aggggctctc gaaaattctg aaggccatcg
     5701 ggcgccgcga tgtcccggtg atcgcgcatc cggcgctttt ccgcccgaat tttatcacgg
     5761 ccccgttctt ccggcacgtg ggggtgatgc agggggacga accgctcgac attcaggcgg
     5821 ccggagggag cctctttctt ttctcggagc agttgcagct gatgccaggt cttgagacga
     5881 cgggcgaagt tccgcgcacg acggatttcg aagaggttgg catcgcgctc tgtacg
//
LOCUS       DAOPKU010000139         5901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14517_length_5901_cov_4.765652, whole genome shotgun sequence.
ACCESSION   DAOPKU010000139 DAOPKU010000000
VERSION     DAOPKU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14517_length_5901_cov_4.765652"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK849_05705"
     CDS             <1..543
                     /locus_tag="PK849_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPE65656.1"
                     /translation="VVGLGNVGRSVALRMKAFGMNVTGVNRTPPSEEGVVDDFFPLSG
                     LEQAVKGRRFVVVALALNAATENIFGESFFKAMDRDAFFINVARGGLVCRDALENALK
                     EQWIAGAGLDVLWEEPHSPEDPILTHPAVTVTPHIGGVNDASFEGVLSFIVRNIDLLA
                     SGKNPLSRLDIKGAGASQSR"
     gene            548..1468
                     /locus_tag="PK849_05710"
     CDS             548..1468
                     /locus_tag="PK849_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65657.1"
                     /translation="MTAYLIKRIFYFAAVLAVATGTIWVYQYWWEQNALESPDVVSAV
                     PYTYIERIPVKGVLIWEETVLVSRWDGNLSYPEPGTRRVARGETVAVIGSSSGKMAVR
                     SESVGYFVPGLDGAEGEWRYSFHWPGMAPLPASPAFAEFSSGVFVRRGDAVGKLISQP
                     QELRCVLYADVTPALQKDIQTGFVRVKTEAHGWPGRAAVRASRFLGTKMKLYLTLPFF
                     SLENVKSREIALFLETGEHVGVAVPESSVRYREGKLGVFQVEGNIVKFKEIEGLPVEG
                     HRFFVQKGLQSGNIVVLNAERAKEGKIRLW"
     gene            1462..2154
                     /locus_tag="PK849_05715"
     CDS             1462..2154
                     /locus_tag="PK849_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPE65658.1"
                     /translation="MVSAIHNNLQRVREKLADAVRKSGRNDEDVRLVAVTKTYPVEDI
                     LEAVRCGVVSIGENRVQELSEKRSLWSGPSIEWRFIGHLQGNKVRKVLEFASTVDSLD
                     SLSLARALSRVAEEKKMRLPVLIEVNTSGEESKHGTAPSDAAPLLESVMKECPALQVE
                     GLMTVGPLTDDEAEVRRAFALLRGIRDSLSGAFGLRLKELSMGMSGDFEWAVDEGSTM
                     VRIGSAIFGKRT"
     gene            2179..2559
                     /locus_tag="PK849_05720"
     CDS             2179..2559
                     /locus_tag="PK849_05720"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPE65659.1"
                     /translation="MLERMMAFLGLVDPAAEEEEGAGETAELETREARVLKPVPESAV
                     ILIARGLEGVQRKEELALALRKGKLLLVDLRGLEREEGQSLLDFLCGVAFANRGTVLR
                     AASGIFLAAPRKSMIEEWEEEDGA"
     gene            2552..3055
                     /locus_tag="PK849_05725"
     CDS             2552..3055
                     /locus_tag="PK849_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPE65660.1"
                     /translation="MPDLLTARDVESKRFSKAMRGYSATEVDQFLDQVAEDIQKYCVQ
                     YADLERYAARLEDKIREFESLKETLSGTLLMAQKTADAKEEAANRQSEAIILEAKSKA
                     EQIIQEAEASKERERKEINHIRQLKQEFKADFCALLSRFSSLIERDEEDPDENDGAEP
                     TEDDVLK"
     gene            3052..5127
                     /gene="recG"
                     /locus_tag="PK849_05730"
     CDS             3052..5127
                     /gene="recG"
                     /locus_tag="PK849_05730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPE65661.1"
                     /translation="MTIRRADLSDPVRFVKGVGPSRERQLANLGVFTAEDLLFLFPRR
                     YEDRRTLTPLSSLRPGTATTVIARVVAVEKRTTAKKQLSLTTVLISDGQGMAKAVWFN
                     RVGLENTFRSGLRVALFGKVELRNGVPQLVAPEFEVLEEDDGEEELLSIVPLYPATAG
                     LHQKTLRRIVRTALEEYLPLLEEFLPVSLRERRRLCELTEAMREIHYPTSRENWKTAR
                     RRLVFDEFFLLQAGLALRRNRAEKPLERAPSLVRGHFVEEYMNRFLPFVLTEGQRSAV
                     EDILSDMERTAPMNRLLQGDVGSGKTVVALIALLAALDGECQGALLAPTEILARQHFL
                     RLSPVFTALGIPCALLTGSLGVKEKKEVYAGLESGEIAVVIGTHALFSEGVSFARLGL
                     AIVDEQHRFGVLQKHTFRAKGSAPHVLVMTATPIPRTLTLTVYGDLSVSVISGMPPGR
                     KGVKTSAVSPKKLPAVLRFLKERFHLGERAYWICPLIEESDSDLSAATARFEELSAEF
                     GAYGVGLLHGRLPLAEKERAMEDFRSGVTRLLVSTTVVEVGVDVPEATVMVVEDAVRF
                     GLSQLHQLRGRIGRGNTEGFCILVCTPSTPESRQRIEAFCSSDDGFAIAEADLRLRGP
                     GEVCGVRQSGVTDFRIADLLKDQEILRLARAEAFALVGEDPELRNTPLLAERVFHVLG
                     RSLNLVETA"
     gene            5111..5569
                     /locus_tag="PK849_05735"
     CDS             5111..5569
                     /locus_tag="PK849_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPE65662.1"
                     /translation="MSKQPERKKIPGAKNPEAYLHSLLSNGVWTKAVVLRKLQGRGIP
                     EEEAEKLIDKYEEAGFFDDGAYALLFVESHPEWGVYRLRDELRRRGVAQEHILKALEG
                     KDEDENAAALAGEWIRMGIDRRKIGERLLRRGFSGNACRNALERACEGEL"
     gene            5664..>5901
                     /locus_tag="PK849_05740"
     CDS             5664..>5901
                     /locus_tag="PK849_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HPE65663.1"
                     /translation="MNFFTAIVALVVGVAAGIGAGFLLSKSFGIKNIENAKAQARAIL
                     REAAMAAEQGKREKLTEAKEEIFKLRQEVEREGKE"
BASE COUNT         1301 a         1416 c         1860 g         1324 t
ORIGIN      
        1 gtggtaggtc ttggaaatgt ggggagatcc gtagcgcttc gtatgaaggc gttcggcatg
       61 aatgtgacgg gcgtgaatcg taccccccct tcggaagagg gggttgtgga tgatttcttc
      121 cctctttccg gtctggaaca ggcagtgaag gggcgccggt tcgtagtggt tgcgctcgcg
      181 ctgaatgctg ctaccgagaa tattttcggc gagtcttttt ttaaagcgat ggacagagac
      241 gccttcttca tcaatgtggc aagaggaggg cttgtttgca gagacgcgct tgaaaacgcc
      301 ctgaaggagc agtggattgc cggcgcaggc ctcgatgtgc tgtgggagga accgcattct
      361 ccggaagatc ccatccttac tcaccccgcc gtcacggtaa cgccccatat cggtggagtg
      421 aacgacgcat cctttgaagg cgtgctctcg tttatcgttc gcaatatcga tcttcttgct
      481 tcggggaaaa atccgctctc gcgtctcgac atcaaaggag cgggggcttc ccagtcccgt
      541 taggcgtatg actgcgtacc ttatcaagcg aatattctat tttgccgcgg tgcttgccgt
      601 tgcgacggga acgatatggg tgtatcagta ctggtgggag cagaacgccc ttgaaagccc
      661 tgacgtagta tcggccgtgc cctatacgta catcgaaaga atcccggtca agggagtctt
      721 gatatgggaa gaaaccgttc ttgtttcacg gtgggacgga aatctttcgt atccggagcc
      781 gggaacgaga agagtcgcga ggggcgaaac cgttgcggtt atcggttcat cctcggggaa
      841 aatggctgtt cgttcagagt ctgtcggata ttttgttccc ggcctggacg gggctgaagg
      901 agagtggcgg tattcgttcc actggcccgg tatggcgccg ctgccggcgt ctccggcttt
      961 tgccgaattt tcatccgggg tgtttgtccg ccggggagat gccgtgggaa agctcatatc
     1021 tcagcctcag gagcttcggt gcgtcctcta cgccgatgtg acgcccgcgc tccaaaaaga
     1081 tatacaaaca ggttttgtgc gtgttaagac cgaggcgcac gggtggccgg gaagggctgc
     1141 cgtccgtgcg tcgcgttttt tgggtacgaa aatgaagctc tatcttacat tgccgttctt
     1201 ttctctcgag aatgtcaaat ccagggagat tgcgctgttt ctcgagacgg gggagcatgt
     1261 cggtgttgcc gtgccggaat cctctgttcg atatcgcgag ggaaagctcg gggtgttcca
     1321 ggtggagggg aatatcgtaa aattcaagga gatcgaaggt ctgcccgtgg aaggacatcg
     1381 tttttttgtc caaaaggggt tgcagtcggg caatatcgtc gtcctcaatg cagaaagagc
     1441 gaaggaaggg aaaatccgac tatggtgagt gcaatacaca ataaccttca acgtgtgcgg
     1501 gaaaaacttg ccgatgctgt ccgcaaaagc ggaaggaatg acgaagacgt tcggcttgtc
     1561 gcggtgacaa aaacctatcc ggtggaggat attcttgaag ccgtccggtg cggcgtcgtc
     1621 tcgatagggg agaaccgggt acaggaactc tcggagaagc gaagtctttg gagcggtcct
     1681 tccatagagt ggcgttttat cgggcatctg caaggaaaca aagtgaggaa ggttcttgaa
     1741 tttgcatcca cggtcgattc cctggattca ttgtcgctcg ccagggctct ctcgcgtgtt
     1801 gcggaagaga aaaaaatgcg tcttccggtg ctcattgagg tgaatacttc cggagaggag
     1861 agcaagcatg gtactgctcc ttccgacgcg gcgccgcttc ttgagagcgt gatgaaggaa
     1921 tgtcccgcgc ttcaggtaga aggcctgatg acggtgggac ctttgaccga tgacgaggcg
     1981 gaggtacgaa gagccttcgc gctgctgcgg gggatcaggg attctttgag cggagcgttc
     2041 ggtctccgct tgaaagagct ttccatgggg atgagcggtg attttgaatg ggctgttgat
     2101 gagggaagta cgatggtccg tatcggctcc gctatcttcg gaaaacgtac ctgatacgcc
     2161 gcagcgggag gtgatgcctt gttggaacga atgatggcct ttctggggct cgttgacccg
     2221 gctgccgaag aagaggaagg cgcgggggag acggcggaac tcgaaacaag agaggcccgc
     2281 gtactgaagc cggttcctga aagcgcggtg attcttatcg cccgcggtct tgagggagta
     2341 caaagaaaag aagagcttgc cctggctctc cgtaagggga aacttctgct cgtcgatctt
     2401 cggggtcttg aacgggaaga gggacagagt ctgctcgatt tcctctgcgg agtggctttt
     2461 gcaaacagag gtactgttct gcgggcagca agcgggattt ttctcgcggc gccgcggaag
     2521 agcatgatag aagaatggga ggaagaagac ggtgcctgat ctgctgacag ctcgcgatgt
     2581 ggaaagcaaa cgcttttcaa aggcgatgag agggtattcc gcaacggaag ttgaccaatt
     2641 cctcgaccag gttgccgagg atatccagaa gtactgcgtc cagtatgccg atcttgaacg
     2701 ctatgccgcc cgcctggagg acaagatccg ggagtttgaa agcctgaaag aaacgctctc
     2761 cggaactctg ctcatggcgc agaaaactgc ggatgccaag gaagaagctg caaaccgcca
     2821 gtccgaggct atcatactgg aggcgaagtc gaaggcggaa cagatcattc aggaggcgga
     2881 agcctcgaag gagcgggagc gcaaggagat caatcatatc aggcagctca aacaggagtt
     2941 taaagccgac ttttgcgcac tcctctcccg tttttcttcc cttattgaac gcgatgagga
     3001 agatcccgac gagaacgacg gtgctgaacc gacggaggac gatgtgctca agtgactatt
     3061 cgccgagcgg acctgtccga ccccgttcgt ttcgtgaagg gagtcggccc ttccagagaa
     3121 cgtcagttgg ccaacctcgg tgttttcacg gcagaggatc tcctgtttct ttttccccgc
     3181 cggtacgaag acaggagaac gctgacgccg ctttcgtctc tcaggcccgg aacagccacg
     3241 acggtgatcg ccagagttgt cgctgttgaa aaacggacaa cggcaaaaaa gcaactctct
     3301 ctgaccactg tgctgatttc cgatggccag gggatggcga aggctgtctg gttcaacaga
     3361 gttggacttg agaacacctt tcgttcagga ctcagggttg ctctgttcgg taaggtggag
     3421 ctgcgcaacg gcgttcctca gcttgtagcg cctgaatttg aagtgcttga agaggatgac
     3481 ggagaagagg agctgctctc gattgttcct ctgtatccgg cgactgcggg gctgcatcag
     3541 aagacgctgc gccgcattgt gcggacagct ctggaggagt atcttcccct gctggaagag
     3601 tttcttcccg tatctttgcg ggagcgccgc cgcttatgcg aattgactga ggcgatgagg
     3661 gaaatacact acccgacgtc gcgggagaac tggaagactg ccaggagacg ccttgtcttc
     3721 gacgaattct ttctcctgca ggcggggctt gcgctgcgaa ggaacagggc cgaaaaaccc
     3781 cttgaacgag ccccttcgct tgtccgtggg cattttgtcg aagagtatat gaatcgcttc
     3841 cttccgtttg tgcttacaga aggacagagg agcgccgtgg aggacatcct ctcggatatg
     3901 gagcgcacgg cgcctatgaa tcgtcttctt cagggagacg tgggatcggg aaagaccgtc
     3961 gtcgcgctta tcgcattgct cgccgcgctc gacggggagt gtcagggggc tcttctggcc
     4021 ccgaccgaga ttcttgcccg ccagcacttc ctccgccttt cacctgtttt tactgctctt
     4081 ggaattccgt gcgccttgct gaccggctct ctcggcgtca aagaaaaaaa agaggtatac
     4141 gccggtcttg aatccggcga aatagccgtt gttataggga cgcacgctct cttctcggaa
     4201 ggagtatcgt ttgctcgatt gggccttgcc atcgtcgacg aacagcaccg tttcggcgtc
     4261 ctccagaagc ataccttccg cgccaaggga agcgcgccgc acgttttagt gatgacggcg
     4321 acgccgattc ctcgcacgct gacgctgacc gtctacggag atctctctgt atccgtgatc
     4381 tcaggtatgc cgcccggaag aaaaggagtg aagacgagtg cggtttcgcc gaaaaaactg
     4441 cccgcagttc tgcgtttttt aaaagaacga tttcacttgg gagagcgcgc gtactggata
     4501 tgccctctca tcgaagagag cgactccgat ctttccgcgg cgacggcgag gttcgaagag
     4561 ctctctgcgg aattcggagc ctatggagtg gggctgctcc acggaagact cccgcttgcg
     4621 gagaaggaac gggcgatgga ggattttcgg agcggcgtca cacggcttct tgtttctacc
     4681 acggtggtcg aggttggggt ggatgttccc gaggcgacag tgatggttgt cgaggacgcg
     4741 gtccgattcg ggctttccca gcttcatcag ctgagaggta gaatcggcag aggaaatacg
     4801 gagggattct gcattcttgt gtgtacgccg tcgactccgg aaagcaggca gagaatcgag
     4861 gcgttctgct cctcggatga cggctttgct attgcggagg cggatctccg gcttcgtggt
     4921 cccggggaag tctgcggcgt ccgccagtcc ggtgtgacgg attttcgtat tgccgatctt
     4981 ttaaaagacc aggagatcct ccggcttgcg cgagcggaag cattcgccct tgtcggggag
     5041 gatccggaac tcaggaatac gccgcttctg gcggagcgtg tatttcatgt cctcgggagg
     5101 tcgctgaatc ttgtcgaaac agcctgaaag aaaaaaaata cccggcgcaa agaacccgga
     5161 ggcatatctg cattcgctgc tttcaaacgg cgtctggacg aaggctgtcg ttcttcgcaa
     5221 gcttcaaggc agggggattc cggaggaaga agcggaaaag ctcatagaca agtacgagga
     5281 agccggtttt ttcgatgacg gcgcctacgc ccttcttttt gtggaatcgc atccggaatg
     5341 gggagtatac agacttcggg acgagttgcg gaggagaggc gttgcgcagg agcatatact
     5401 caaggccctt gaaggaaaag atgaggatga gaatgcggct gcgcttgccg gcgaatggat
     5461 ccggatggga atagatcggc gaaagatagg cgagagactg cttcggcgcg gtttttcggg
     5521 gaatgcatgc agaaatgctc ttgaaagggc ttgtgagggg gaactttgac ctgtaaaata
     5581 aagaggtcat gggagagagt gccttccatg gcgtggagta caaaggcacc ttgaaaatat
     5641 aatcgaggag tgatcatgat actatgaact tttttactgc aattgtggcg ctcgttgtag
     5701 gagtcgccgc tggaattgga gcggggttcc tgctttccaa atctttcgga atcaagaaca
     5761 tcgaaaatgc aaaggcgcag gctcgggcta ttttgcggga agccgccatg gcggccgagc
     5821 aggggaaacg cgagaaactc accgaggcga aagaagagat cttcaaactt cgccaggagg
     5881 tcgaacgcga aggtaaggaa c
//
LOCUS       DAOPKU010000140         5834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14742_length_5834_cov_4.549057, whole genome shotgun sequence.
ACCESSION   DAOPKU010000140 DAOPKU010000000
VERSION     DAOPKU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14742_length_5834_cov_4.549057"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /gene="dnaG"
                     /locus_tag="PK849_05745"
     CDS             complement(<1..970)
                     /gene="dnaG"
                     /locus_tag="PK849_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPE65664.1"
                     /translation="MRSDDVRRVKDRLDIVEVIGDYVRLKKAGNNFLGLCPFHDEKTP
                     SFNVSPSRQTFHCFGCGQGGDVFSFIMEKENLSFREALETLAERAGVQLSSEGARTLR
                     ERSLFDIMERACSHFRSLLEKPEGEHAAAYLARRNLPREAWRNFELGWASPSWDALWK
                     ALANEGVSFREAFDAGLVLEGKNGPYDRFRGRIIFPVRDISGRLLAFGGRLLDGEGAK
                     YINSPEGVLYSKRRSLYLLNRAKEAARSKDRLILVEGYMDAIRLHLNGFEETAASLGT
                     SLTEEQALLIRRLTDRCFICYDSDIAGQEASIRGMYVLQKAGLDVRV"
     gene            complement(1047..3068)
                     /locus_tag="PK849_05750"
     CDS             complement(1047..3068)
                     /locus_tag="PK849_05750"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPE65665.1"
                     /translation="MLLFLLTLIVAVTGALALLYAYLTYSQKVLFGCTWFKVSEAPEE
                     APEGEMIEKTARVHELSEIIHKGAMAFLYREYKYLLPFLLVVGVLLIYFIGFAAGFSF
                     IIGGLCSAAAGYAGMIVATRTNGKTALAATRGMNEALLTAFRGGSVMGMSVVGIGVLG
                     IVFCYALFRDPNLITGFGMGASTIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDP
                     RNPAVIADNVGDNVGDIAGMGADLFESYVNSIIAAMALGLALFGWKGVSFPMVMAGVG
                     ILAAIVGTRFVKVEEGGNPAVALRKGTFVTGGIMIVGAFIFSFLMFGALSLFWSVLSG
                     VAVGVLIGFVTEIYTSGDYASVKHIAHASKTGPATVILEGVAVGMKSTVVPVILICAA
                     TLVSYYFAGLYGIACAAVGMLSITGMALSVDAYGPIADNAGGIAEMSHLPPEVRKITD
                     KLDAIGNTTAAMGKGLAIGSAALTALALFAAYAHSVKLTAIDLNDPKVMVGLFLGGML
                     PFLFSALSIQAVGRAAEAMIDEVRRQFREIPGIMEGTGRPEYEKCVDISTAAALKEMI
                     IPGLLAVLSPLAVGFGLGAQALGGLLAGSIVTGVMLAIFMSNAGGAWDNAKKYIEEGN
                     FGGKGTPEHAAAVVGDTVGDPFKDTAGPSLNILIKLMSVVALVLAPLFI"
     gene            complement(3221..3856)
                     /locus_tag="PK849_05755"
     CDS             complement(3221..3856)
                     /locus_tag="PK849_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HPE65666.1"
                     /translation="MPLLIAVSGFKDSGKTSLARALLTELSGRGLSLAFMKHTDRDVL
                     SPAGTDTGGIESQGIPCAYWGNDGLRMELPAAAPNSDTIAALFPEKDIVIVEGAKSIS
                     MPRIWVGSAESAPPEVKGVFACFDRAAEKGDGRFLYVPGEENELADRIAAFHERFANV
                     RCVRVAVRGKRLPLKPFVAEMIRGSIAGLLLPLKGIDSLKEGADIHLARSE"
     gene            complement(3929..5041)
                     /locus_tag="PK849_05760"
     CDS             complement(3929..5041)
                     /locus_tag="PK849_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPE65667.1"
                     /translation="MGGKGVSSGEGQKTQMSRGWFLPFLFIFLALAYLSFTVSLPLLK
                     PLAWAVLLSFTVYPVFKRMRDRFFPRSITGNWAATIATGLIVLLLVIPSLFTGFVAAR
                     EGVRFYETVADVIAGMESSGESALTAFLPEQIVERMHPWMERYPLLRTGLQQAAGWAA
                     TTAVAASRAVLGSTFTFLYYLAVIIVVSFFLIRDGHLIIEYLKDIMPLPAKESAAFFV
                     RAKQVLQAVVYGVIVTALAQGLAGAVGWRIVGLPSPVFFGALMALFAMIPFLGTALVW
                     VPGALYLFYMSETTQALILLAWGVLVVSMVDSLLRPIFISGGGKVHLLIVFIGVIGGL
                     AAWGILGLFMGPLVVTLFVFLLESYRTMWKTFFVSR"
     gene            complement(5051..>5834)
                     /gene="guaA"
                     /locus_tag="PK849_05765"
     CDS             complement(5051..>5834)
                     /gene="guaA"
                     /locus_tag="PK849_05765"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPE65668.1"
                     /translation="LLRLNEAEQVLEQYKALNLKVHYVDATDVFLGALAGVSDPERKR
                     KIIGERFVRVFEAEAEKIEGAKWLLQGTLYPDVIESGHKGKNASVIKSHHNVGGLPED
                     MKLLVLEPLRDLFKDEVRSTGRMLGIPEEIVARQPFPGPGLAVRCLGEITRERLDTLR
                     RADAIFREELSGWEGVSSVWQSFVALLPVRTVGVMGDSRTYSEAAVLRAVESHDGMTA
                     DWVRIPWEILDRTSRRICNEVHGINRVALDVTSKPPATIEWE"
BASE COUNT         1213 a         1872 c         1732 g         1017 t
ORIGIN      
        1 cgacgcggac gtccagtccc gccttctgca gcacgtacat tccgcggatg gacgcctcct
       61 gccccgcgat atccgagtcg tagcagatga agcagcggtc ggtgaggcgt cggatcagca
      121 gcgcctgttc ctccgtgagg gaggtgccca gagaggccgc agtctcctcg aaaccgttca
      181 ggtggaggcg tatggcgtcc atgtacccct ccacgaggat gagcctgtcc ttcgagcgcg
      241 ccgcctcctt cgccctgttg agcaggtaga gcgaacgccg cttgctgtag agcacccctt
      301 cggggctgtt gatgtacttc gccccttcgc cgtcgagcag ccgtcctccg aaagcgagga
      361 gccgtccgga aatgtccctg acggggaaga tgattcgtcc gcggaagcgg tcgtacggcc
      421 cgtttttccc ctccagtacg agccccgcgt cgaaagcctc gcgaaaggag acgccttcgt
      481 tggcgagcgc cttccagaga gcgtcccagg agggggacgc ccagccgagc tcgaagttcc
      541 gccacgcctc gcgtgggaga tttcgccggg cgaggtacgc cgcggcgtgc tccccttcgg
      601 gcttttcaag caaggagcgg aagtgggaac aggccctctc catgatgtcg aagaggctgc
      661 gctccctgag cgtacgcgcg ccctcggagg agagctgcac tccggccctt tcggcgaggg
      721 tttcgagcgc ctcccgaaag gagaggtttt ctttttccat gatgaaggag aagacgtccc
      781 ctccctgacc gcagccgaaa cagtggaacg tctggcgcga cggggagacg ttgaacgagg
      841 gcgtcttctc gtcgtggaag gggcagaggc cgagaaagtt gttccccgcc ttcttcagac
      901 ggacgtaatc gccgatgacc tccactatat cgagccggtc tttcacgcgg cgaacatcgt
      961 ctgatctcat gagcggcgcc tccgaagaaa aaataagggg gaggtcgccc tccccctctc
     1021 gcagatattg tactcccttt ttcggattag atgaagagcg gcgccagcac gagcgccacg
     1081 accgacatca gcttgatgag gatgttgagg ctcgggccgg cggtgtcctt gaacgggtcg
     1141 ccgaccgtgt cgcccacgac cgcggcggcg tgctccggcg tccctttgcc cccgaagttc
     1201 ccctcttcga tatacttctt cgcgttgtcc catgcgccgc ccgcgttgga catgaagatg
     1261 gcgagcatca cacccgtcac gatggaaccg gcgagcaggc cgccgagcgc ctgggcgccg
     1321 aggccgaagc cgacggccag cggggagagg accgcgagca ggccggggat gatcatttcc
     1381 ttcagcgcgg cggcggtgga gatgtcgaca cacttctcat attcgggccg tcccgttccc
     1441 tccatgatgc cggggatctc gcggaactgg cgccgcacct cgtcgatcat ggcttccgcg
     1501 gcgcggccga ccgcctggat cgaaagcgcg ctgaagagga acggaagcat gccgccgagg
     1561 aagagaccga ccatgacctt cgggtcgttc aggtcgatgg ccgtcagctt gacggagtgc
     1621 gcgtacgcgg cgaagagtgc gagcgcggtg agcgccgcgg agccgatcgc cagtcctttg
     1681 cccatcgccg cagtcgtgtt gcctatggcg tcgagcttgt cggtgatctt acggacctcc
     1741 ggcgggaggt ggctcatctc ggcgatgccg cccgcattgt cggctatagg cccgtaagcg
     1801 tccacactca gggccattcc tgtaatcgag agcataccga cagccgcgca ggcaatgccg
     1861 tagaggcctg caaagtagta gctgacaagg gtcgcggcgc atatcagaat aacgggaacg
     1921 accgtcgact tcatgccgac cgccacgcct tccagaatca ccgttgcggg accggtcttc
     1981 gacgcatgcg ctatgtgctt cacggaggcg tagtctcccg acgtgtagat ctcggtgacg
     2041 aaaccgatga gaacgcccac cgccactccg gagagcaccg accagaagag cgaaagagcg
     2101 ccgaacataa ggaaggagaa gatgaacgcg ccgacgatca taatgccgcc cgtgacgaag
     2161 gtccccttac gcagcgcgac ggcggggttg cccccctcct ccaccttgac gaaacgcgtt
     2221 ccgacgatcg cggccagaat gcccacgccg gccatgacca tcgggaagga gacgcctttc
     2281 cagccgaaga gagcgagtcc gagcgccatc gccgcgatga tggagttcac gtacgactcg
     2341 aagaggtccg cgcccatgcc cgcgatgtcg ccgacgttgt cgccgacgtt gtccgcgatg
     2401 accgccgggt tccgggggtc gtcctcggga atccccgcct cgaccttgcc gacgaggtcg
     2461 gcgccgacat ccgctgcctt ggtgtagata ccgccgccga cacgcgcaaa gagcgcgatc
     2521 gtactggcgc ccatgccgaa tcccgtgatc aggttaggat cgcggaagag cgcgtagcag
     2581 aagacgatac cgaggacgcc gatgcccacg accgacattc ccatgacgct gccgcctctg
     2641 aaggcggtga gcagggcttc gttcatcccg cgcgtagcgg caagagccgt cttgccgttg
     2701 gtcctcgtgg ccacgatcat tccggcatac cctgcggccg cgctgcacag accgccgatg
     2761 atgaaggaga agcccgccgc gaaaccgatg aagtagatca ggagcacgcc gaccaccaga
     2821 aggaacggca gaaggtattt atactccctg tacaagaagg ccatcgcgcc tttgtggatg
     2881 atctcggaga gctcatgaac ccgcgcagtc ttctcgatca tctctccctc gggcgcttcc
     2941 tcgggcgcct cagacacctt gaaccaggtg catccgaaca ataccttctg gctgtacgtc
     3001 aggtaggcgt agagcaacgc aagagctccc gtgaccgcga caatgagtgt caacaagaaa
     3061 agcagcattt cacagactcc tcccaatagg aaaaatatga aaaaaccatc ttttttccgt
     3121 ccggtcgtcg aacagcgagg cgagttaggg acggcataca acaactgaca ttatagtaat
     3181 ccttaatgta aaaaacaagg ttcatttcta aaaaacagcg ttattcacta cgtgcgagat
     3241 gaatatcagc cccttccttc agggaatcta tccctttcaa cggtaaaagc agtcccgcga
     3301 tgcttccgcg gatcatctcg gcaacaaagg gtttgagcgg aagccgcttc ccgcgaacgg
     3361 cgaccctgac gcaccgcacg ttcgcaaatc gctcatggaa cgccgcaatt cgatccgcca
     3421 gttcattctc ctcgcccgga acatagagaa aacgcccgtc ccctttttcg gccgccctgt
     3481 cgaaacacgc aaaaacgccc ttcacttccg ggggcgcgct ttccgcggaa ccaacccaga
     3541 tcctcggcat ggaaatgctc ttcgcaccct cgacgatgac gatgtccttc tccggaaaga
     3601 gcgccgcgat cgtatcactg tttggagcgg ctgcaggcag ttccatcctg aggccgtcgt
     3661 tcccccagta ggcgcacggt atcccctgag actcgattcc gccggtatcc gtgccggccg
     3721 gggagagcac gtccctgtcc gtatgtttca tgaaggcgag ggagagcccc cgtccggaaa
     3781 gctccgtgag aagcgcgcgc gcaagcgacg tcttccccga atccttgaaa ccggagactg
     3841 cgatcaggag aggcatggcc cgcctccttc gatatgtccg ttcactcatc gttcctcctt
     3901 cttgatgatt gggcacgttc cggcggcgtt accgggagac gaaaaaagtt ttccacatcg
     3961 tccggtagct ctcaagcaga aagacgaaca gcgtcaccac gagcggcccc ataaaaagcc
     4021 ccagaatccc ccatgcggct aggccgccga tgacgccgat gaagacgatg agcaggtgta
     4081 ccttcccgcc gccggagatg aaaatcggcc ggagcaggct gtccaccata ctcaccacga
     4141 gcacgcccca tgcaagcaat atgagagcct gcgtcgtctc cgacatgtag aagagataga
     4201 gcgcccccgg aacccaaacg agagcggtcc ccagaaaagg aatcatcgcg aagagcgcca
     4261 tcagagcccc gaagaagacc ggactcggaa gaccgacgat ccgccatccg acggctccgg
     4321 ccaagccttg agccagagcg gtgacgatga cgccgtagac cacggcttgc aacacctgtt
     4381 tcgcgcggac gaagaaggcg gcgctttcct tcgccggaag cggcatgatg tccttgaggt
     4441 actcgatgat aaggtggccg tccctgatga gaaagaaact caccacgatg atcacggcga
     4501 gatagtagag aaaggtaaag gtactcccta aaaccgcgcg cgatgccgcg acggcggttg
     4561 tcgccgccca tccggccgcc tgctgcaatc cggtgcgcaa cagcggatac cgctccatcc
     4621 acgggtgcat tctctcgacg atctgctcgg gcaggaatgc ggtcagcgcg ctctcgcccg
     4681 acgactccat ccccgcgatc acatcggcga cggtttcgta gaatcggacg ccctcccgcg
     4741 ccgcaacgaa ccctgtaaaa agagagggaa tcacgagcag caggacgata agccctgttg
     4801 caatcgtagc ggcccagttc cccgttatac tacgcgggaa gaagcggtcc cgcatccgtt
     4861 tgaagacggg gtagacggtg aacgaaagca gcaccgccca cgccagcggc ttgagcagag
     4921 gaagcgagac cgtgaaggag aggtacgcaa gcgcaaggaa gataaagaga aaaggaagga
     4981 accaccctct cgacatctgc gtcttctgtc cttctccgga agaaacgccc ttccctccca
     5041 tcgccgtcgc ctactcccac tcgatggttg ccggcggctt gcttgtcacg tcgagcgcga
     5101 cccggttgat cccgtgcact tcgttgcata tcctccgcga ggtcctgtcg agaatctccc
     5161 agggaatccg cacccagtcg gccgtcatgc cgtcgtgcga ctccacggcg cggagcaccg
     5221 ccgcctccga ataggtgcgc gaatccccca tcacgccgac cgtcctgacg ggaagcagcg
     5281 ccacgaagga ctgccatacg gaagaaacgc cttcccaccc ggaaagctcc tcccggaaga
     5341 tggcgtccgc ccggcgcagc gtgtcaaggc gctcgcgcgt tatctctccg aggcagcgga
     5401 cggcgaggcc gggaccgggg aagggctggc gcgccacaat ctcctcgggg atgccgagca
     5461 tacgccccgt ggagcgcact tcgtccttga agagatcgcg gagaggctcc agtacgagaa
     5521 gtttcatatc ttcgggcagg ccgccgacgt tgtggtggct cttgatgacc gaggcgttct
     5581 tccccttgtg cccgctctcg atgacatcgg ggtagagcgt tccctgaagc agccacttgg
     5641 cgccctctat cttctccgcc tccgcttcga agaccctgac gaaacgctcg ccgatgatct
     5701 tccgcttccg ctcgggatcg gagacgcctg cgagcgcccc gaggaagacg tccgtcgcgt
     5761 cgacgtaatg caccttcaga ttgagcgcct tgtactgttc gagcacctgt tccgcctcgt
     5821 tcaggcgcaa cagc
//
LOCUS       DAOPKU010000141         5833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14747_length_5833_cov_4.831256, whole genome shotgun sequence.
ACCESSION   DAOPKU010000141 DAOPKU010000000
VERSION     DAOPKU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14747_length_5833_cov_4.831256"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PK849_05770"
     CDS             complement(<1..212)
                     /locus_tag="PK849_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65669.1"
                     /translation="MRRASRGRGVACFLLSVVIVFFAAAGPSSADYSTTGVVGGPHPF
                     TYTVKCAPGDRFEVAVSSDYATSVDIL"
     gene            complement(249..707)
                     /locus_tag="PK849_05775"
     CDS             complement(249..707)
                     /locus_tag="PK849_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65670.1"
                     /translation="MMRYRIIAWTLLLFFAACCVPAESVMPPEVYANMAEKSRIKATA
                     VVESVKALETGKQSTWKSVVFKLRFPFTENVPEQFTGTCYSVDFPWQEPPVGGTLYYY
                     PEAGDLVYVTVGSNGGPITSYTYLNDVMESLFIDNEEKIRYGMGNAWLDL"
     gene            887..2575
                     /gene="ilvD"
                     /locus_tag="PK849_05780"
     CDS             887..2575
                     /gene="ilvD"
                     /locus_tag="PK849_05780"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPE65671.1"
                     /translation="MTNKWRSSEVFAGAPNAGARALWKSMGYCDGDFAERPVIGIANS
                     WNTLVPGHFNLNQVAEQVKKGIHRAGGVAAEFGVIASCDGVCEGHRGMHFILPQREVI
                     ADSVELVVEAHHMDAIVLLASCDKIVPGMLMAAARLDIPAIMITGGPMLGGVEFDGRK
                     TDETSLHEALGMLTAGRISDEEFHSLENLCAPTCGSCSFFGTANTMCCLAEVLGMTLP
                     GSALVPAVHAERLRLACATGERICEMAREGLTARKILNASSIVNGVRATLAMSGSTNA
                     VMHLAAIAAEAEAGVDVMELFSRLEQETPQIVRINPASKWNTEDFWMAGGIPRMLKNM
                     LPIIEKDALTCTGRTVEENVKAFRFPFPKNNEVMKTLESPFSKRGGIAVLRGNLAPDT
                     GVTKPGAIDPTLHVFTGEARVFDSEDDANAAILAGKIHKGNVVVIRYEGPKGGPGMRE
                     MYRAMKYLYGMGLNKSTALVTDGRFSGTNNGCFVGHISPEAAEGGPIAIVRDGDRITI
                     DVIEGKLHLHVDNDEIRERFESWTKPAPKVTRGHLALYSKLASSAAKGAIVRAE"
     gene            complement(2640..3920)
                     /locus_tag="PK849_05785"
     CDS             complement(2640..3920)
                     /locus_tag="PK849_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE65672.1"
                     /translation="MIALMTITFVVCFVMGIPLALVMGITGFAVILAMGVPLQLVAQR
                     MFTGIDSFSLMAVPFFILAGDLMNRGGTTVRIIGFANSLVGHIRGGLAHACIVANMIF
                     AGISGSAVADASAIGSIMIPSMEKNGYDLDFSAALNSAAATIGPIIPPSIIMVIYGVS
                     VNVSVGGLFAAGFIPGIMLGLAMMIVVSRVSRKKNYAMSEGFSGARVRKELKSSVWAL
                     MAPVIILGGILGGVFTPTEAAAVAVIYSFFVGKFVFREITWKDLPGILFQSGITTGAI
                     LLIISLANVFAWVIAANQIPVKLSSMFLSATSNPYVFLLIVNILLLIVGMFMETGAAI
                     ILLAPILAPIATQLGVHPLHFGFMMVLNLAIGMATPPVGVCLFVSCGITGLSLEKVSA
                     AVMRFVLAEIAILTLVTYVAPISLFLPRLLRFIR"
     gene            complement(3917..4447)
                     /locus_tag="PK849_05790"
     CDS             complement(3917..4447)
                     /locus_tag="PK849_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE65673.1"
                     /translation="MKFLEGVLACFDKAAFAAVALLMGSMGVVAFAAVFYRFVLHDPI
                     TWSEEAARYMMVWVTFLGAGYAMGKGKHIGVTMFVEKLPEGVRRKVIFVAEAVIMLFL
                     AAVTVQGVKLIVSLWVQTSPAMDLPMWLPYLAIPVGAVYMFLHLLRLMLSRSAHVLAT
                     ADIELDAALKRKGDDR"
     gene            complement(4549..5580)
                     /locus_tag="PK849_05795"
     CDS             complement(4549..5580)
                     /locus_tag="PK849_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE65674.1"
                     /translation="MKKMFGLMCVVALTGLLAGSALAADFTLKLANAGPADPEDRTVI
                     AVEIFKNHVETKTGGSVKVEVFHASQLGNEKEILKGLALGTIELGTITTGPVPTLFKP
                     IMVFDIPFLFPNQVVAWEVLDGPFGRKLMEEMRKSTGIRTLAISENGYRHFVTGSKQV
                     KSMADMKGLKIRTMENPAHMKMVEALGGAPTPIAFGELYMALQQGVVDGCELPVTLIN
                     NGKYYEVQKHVILNGHLYNPLIMFVNDKVWEKLSQDQKAALYEGAQLFNIAQRAISER
                     QVQSGLLNVQKNGMTVYVPTAAELNEFRSAAQPAVLDYVKAQAGEQWVNEALEAVAAA
                     EAKMAEMLK"
BASE COUNT         1227 a         1821 c         1719 g         1066 t
ORIGIN      
        1 agtatatcca ccgaggtagc gtaatcggat gacaccgcaa cctcaaaccg gtcccccgga
       61 gcgcacttga ccgtatatgt aaaagggtgc ggaccgccga cgacgcctgt ggtcgagtag
      121 tccgccgaag aaggccctgc cgccgcaaaa aacacgatca ccactgaaag caggaaacac
      181 gcgacacctc gtcctctact cgctcttctc atgcccatct cctttccgga aacgcccctg
      241 gctgcacatt aaagatccag ccaggcgttc cccatgccgt agcgtatttt ttcttcattg
      301 tcgataaaga ggctctccat cacatcgttc aggtacgtgt agctcgtgat cggaccgccg
      361 ttcgacccca ccgtgacata gacgagatcc cccgcttcag ggtagtaata gagcgtcccg
      421 cccacaggcg gctcctgcca ggggaagtcg acggagtaac aggttccggt gaactgctcc
      481 ggcacgttct ccgtgaacgg gaatctcagt ttgaagacaa cgctcttcca tgtgctctgc
      541 ttccccgtct ccaaagcctt cacggactcg acaaccgccg tcgcctttat cctcgacttt
      601 tcggccatgt tcgcgtagac ctccggaggc atcacgctct ccgcggggac gcagcacgcc
      661 gcaaagaaca gaagcagcgt ccacgcaata atccggtaac gcatcatgaa tcctccctcc
      721 tctcattcgg atttttagcg catcgcgcag tctttttcag tatagcctct cctgaggata
      781 tgcgcatcgt gattttcgct tttttggatt ttcgcagcct gttgacgaaa cgcccgctct
      841 cccctactat gcagaagaat tgcaattttc cggaggagga ccgttcatga caaataaatg
      901 gagaagctct gaggtgtttg cgggtgcgcc gaatgccggg gcgcgggcgc tgtggaagtc
      961 gatggggtac tgcgacggtg atttcgccga acggccggtc atcggcatcg cgaactcatg
     1021 gaacacgctg gttccggggc acttcaacct gaatcaggtc gccgaacagg tgaagaaggg
     1081 gatccatcgc gcaggagggg tggcggcgga gttcggcgtc atcgcatcgt gcgacggcgt
     1141 ttgcgagggg cacaggggga tgcacttcat ccttccgcag cgcgaggtca tcgccgactc
     1201 ggtcgagctc gtcgtcgagg cgcaccacat ggacgcgatc gtcctgctgg cctcgtgcga
     1261 caagatcgtc cccggcatgc tgatggcggc ggcccggctc gacatccccg caatcatgat
     1321 caccggcgga ccgatgctcg gcggcgtgga gttcgacgga cgcaagaccg acgaaacgtc
     1381 gctccacgag gcgctgggaa tgctgacggc ggggcgcatc tccgatgagg agtttcattc
     1441 gctcgaaaac ctctgcgcgc ccacctgcgg ctcctgctcc ttcttcggca ccgcgaacac
     1501 gatgtgctgc ctcgccgaag tcctcgggat gacgcttccg ggaagcgcgc tggtacccgc
     1561 ggtccacgcg gaacggctgc gactggcctg cgccacggga gagcgtatct gcgagatggc
     1621 ccgcgagggg ttgacggcgc ggaagatact gaacgcctcc tccattgtga acggcgtgcg
     1681 ggcgacgctc gccatgagcg gttcgaccaa cgcggtgatg cacctcgccg ccatcgccgc
     1741 ggaggcggaa gccggcgtcg atgtgatgga gctcttctcc cgccttgaac aggagacgcc
     1801 gcagatcgtg cggatcaacc ccgcgtcgaa gtggaacacg gaggacttct ggatggccgg
     1861 gggcatcccc cggatgctga agaacatgct cccgatcatc gaaaaggacg ccttgacctg
     1921 tacggggcgc accgtggaag aaaacgtaaa agccttccgc ttccccttcc cgaagaacaa
     1981 cgaggtgatg aaaacgcttg agtccccctt ctcgaagcgg ggagggatcg ccgtcctgcg
     2041 gggcaacctc gcgccggaca ccggcgtgac gaaacccggc gccatcgacc cgacgctcca
     2101 cgtcttcacc ggggaggccc gcgtcttcga cagcgaggac gacgcgaacg ccgccatcct
     2161 cgccgggaag attcacaagg gaaacgtcgt ggtcatccgc tacgaggggc cgaagggcgg
     2221 ccccggaatg cgcgagatgt accgcgcgat gaagtacctc tacggaatgg ggctcaacaa
     2281 gagcacggct ctggtgacgg acggacgctt ctccggcacg aacaacggat gctttgtcgg
     2341 gcatatctcg cccgaggccg ctgagggcgg cccgatcgcc atcgtgcgcg acggcgacag
     2401 gatcaccatc gacgtgatcg agggaaagct gcaccttcac gtcgacaacg acgaaatacg
     2461 ggagcgtttc gagtcgtgga cgaagcccgc gcccaaggta accagaggac acctcgccct
     2521 ctactcgaag ctcgcctcct ccgcggcgaa gggcgcgatc gtccgagccg agtgaagctg
     2581 ggacaaaaaa gggggcggaa aacccgcccc ctcggttctt atgcaatgct gcgcttcccc
     2641 taccggatga agcgcaaaag cctcggcagg aagagcgaga tgggagcaac gtaggtcacc
     2701 agggtaagga tggcgatctc cgcaaggaca aagcgcatca ccgccgccga gaccttttcc
     2761 aggctcagcc cggtgatgcc gcagctcacg aagagacaga cccccacggg cggcgtcgcc
     2821 atcccgatgg cgaggttcag caccatcatg aagccgaagt gcagcggatg gacgccgagc
     2881 tgcgtcgcta tgggggcgag gatcggggcc agcaggatga ttgccgcgcc cgtctccatg
     2941 aacatcccga cgatgagcag caaaatattc acgatgagaa gaaagacgta cggattcgac
     3001 gtcgcggaga ggaacatcga cgagagcttc accgggatct ggttcgccgc aatcacccac
     3061 gcgaagacgt tggcgagcga gatgatcaaa aggatcgcgc cggtggtgat gccgctctgg
     3121 aagagaatgc ccggcaggtc cttccacgta atctccctga agacgaactt cccgacgaag
     3181 aaggagtaga tcaccgcgac cgccgcggcc tccgtcggcg tgaagacgcc gccgagaatg
     3241 ccacccagaa taattaccgg ggccatcagc gcccacaccg acgacttcag ctctttcctc
     3301 accctcgccc cggaaaagcc ctccgacatc gcgtagtttt tcttcctcga gacgcgcgaa
     3361 acgacgatca tcattgcaag gccgagcatg atgcccggaa tgaacccggc ggcgaagaga
     3421 ccgccgacgg aaacgttcac cgagacgccg tagatgacca tgatgatgct cggagggatg
     3481 atcgggccga tcgtcgccgc ggcgctgttg agcgcggcgc tgaagtcgag atcgtagccg
     3541 ttcttctcca tcgaggggat catgatggag ccgatggcgg aggcgtctgc cactgcggag
     3601 ccgctgatac cggcgaagat catgttcgcc acgatgcagg cgtgggccag tccgccccgg
     3661 atatgcccca caaggctgtt ggcgaaaccg atgatgcgga ctgtggtccc gccccggttc
     3721 atcaggtcgc ccgcgaggat gaagaacggc accgccatga gcgagaacga gtcgatcccg
     3781 gtgaacatgc gctgcgccac aagctgcaga ggcactccca tcgcgaggat gacggcgaag
     3841 cccgtgattc ccatgacgag cgcgagagga atccccatca cgaaacagac gacgaaggtt
     3901 atcgtcataa gcgcaatcat ctgtcatccc cctttctctt cagggcggcg tcgagctcta
     3961 tatcggcggt ggccagaaca tgagccgacc gggagagcat cagccgcaaa agatgaagga
     4021 acatatacac ggcgcccacg gggatcgcca gatacggcag ccacatggga aggtccatcg
     4081 cgggcgacgt ctgcacccac aggcttacga tgagcttcac accctgtacg gtcaccgcgg
     4141 caaggaagag catgatcaca gcctccgcca cgaagataac cttccgccgc accccctccg
     4201 gaagtttttc aacgaacatc gtgacgccga tgtgcttccc cttccccatc gcgtatccgg
     4261 cccccaggaa ggtcacccac accatcatgt accgcgcggc ctcctcggac cacgtgatgg
     4321 ggtcgtgaag gacaaagcgg tagaagaccg ccgcgaacgc gacgacgccc atgcttccca
     4381 tcaggagagc gaccgcggcg aaggccgcct tgtcgaagca cgccaatact ccctccaaaa
     4441 acttcattgc tccacctctc cttcacagtg aaatggaaaa ggggacgggg caacaacgcg
     4501 ccccttcccc gatgtgtatt cccgacgata aaagaatccg tccggaagct acttcagcat
     4561 ctccgccatc ttcgcctcgg ctgcggcgac agcttcaagc gcttcgttca cccactgctc
     4621 tcccgcctgc gccttgacgt agtcgagcac ggcgggctgc gccgcggaac ggaactcgtt
     4681 cagttcggcg gccgtgggaa cgtagacggt cattccgttt ttctgcacgt tcagaaggcc
     4741 gctctgcacc tgccgctcgg agatcgcgcg ctgcgcgatg ttgaagagct gcgcgccctc
     4801 gtagagcgcg gccttctggt cctgggagag cttctcccaa accttgtcgt tgacgaacat
     4861 gattagcggg ttgtagaggt gtccgttgag gatcacatgc ttctgcacct cgtagtactt
     4921 gccgttgttg atcagggtca cgggcagctc gcacccgtcc acgacgccct gctgcaacgc
     4981 catgtagagt tcgccgaagg cgatgggggt cggcgcgccg ccgagggcct cgaccatctt
     5041 catgtgcgcg gggttctcca tcgtgcggat cttgagcccc ttcatgtcgg ccatgctctt
     5101 tacctgcttg cttccggtga cgaagtggcg gtagccgttc tcgctgatcg ccagagtccg
     5161 tatcccggtg ctcttgcgca tctcctccat cagcttgcgg ccgaaggggc cgtcgagcac
     5221 ttcccatgcg acaacctggt tggggaagag gaaggggatg tcgaagacca tgatgggctt
     5281 gaagagcgtc ggcaccggtc cggtggtgat agtgcccagt tcgatggtcc cgagcgccag
     5341 acctttaaga atctccttct cgttcccgag ctgcgacgcg tggaaaactt cgaccttgac
     5401 cgatccgccg gtcttcgttt cgacgtggtt cttgaaaatc tccaccgcga tgaccgtacg
     5461 gtcttcagga tcggcgggac ccgcgttcgc cagcttcagc gtaaaatcgg ccgcgagagc
     5521 gcttcccgcg agaagtcctg taagagcaac aacacacatg agtccaaaca tttttttcat
     5581 tttttaccct ccctctaaac tttcataaat ttatcccggc attataaaac aaagtttttc
     5641 acttgcaaag gggaatgtgt tttttcaccc ccgatccgcg tagaagctct ctccctgaga
     5701 gagatgcagc atcactcttc caaccgcccc cgacgccgag gtgagcgaaa ggggcagaag
     5761 cttgtagacc gctgtcggaa cgttcagggt aatggtctcg ttctttctgt cgaaggcata
     5821 ggcatccttt ccc
//
LOCUS       DAOPKU010000142         5831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14759_length_5831_cov_4.169494, whole genome shotgun sequence.
ACCESSION   DAOPKU010000142 DAOPKU010000000
VERSION     DAOPKU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14759_length_5831_cov_4.169494"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1348
                     /gene="upp"
                     /locus_tag="PK849_05800"
     CDS             248..1348
                     /gene="upp"
                     /locus_tag="PK849_05800"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPE65675.1"
                     /translation="MKIAVGSDHAAFALKNYLLEHLRQKSGIESVDMGVDTDAVSCDY
                     PDVAFRVGEAVVSRQADRGLLLCGTGVGMSIAANKIPGVYAAHCHDVETARFSRRHNN
                     ANVLTMGARIISQELAREIIDVWLETDFEGDRHIRRLSKILDYEHKICPSCTFSEKGK
                     TVVFEHPLVQHKVSIIRDKNTSVKEFRELVQEIAGLMVYEITRNLPLVKIEVETPLAK
                     TEAYALEGKKLAIVPVLRAGLGMVDGILQLVPNAKVGHIGLYRDPETLLPVEYYCKLP
                     GDISERDILVLDPMLATGGSAAAAISLVKARGGKKVSLVCIIAAPEGVSKVHAEHPDV
                     DIYAAALDSHLNDHGYIVPGLGDAGDRLFGTK"
     gene            1358..2251
                     /locus_tag="PK849_05805"
     CDS             1358..2251
                     /locus_tag="PK849_05805"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPE65676.1"
                     /translation="MDSLVAPLSVSIPLLFLWGVCVTPLSILLSNRYRIVDQPGERKI
                     HTSVTPRGAGIVLWLGFLLWSLYAVEEAPLLRFMAVGSTLVFLSGYWDDMKTLNPAIR
                     LTFHIVAAGLFLLFLPVSLVHIPVCLLWITGMTNAFNLIDGANGLCLMMFIVASSVFA
                     ACGFPFFFLPVASLAAGVLLWNFPRAKTFLGDGGTTLLGYLYSSLCLYSLTPFLEDVH
                     VFELPFFLLMAGGIPAADTLFAIMRRLAMRKSPFSPDRGHIHHRLLDRGWSPVLTVMI
                     LSVAQLLVVAGGVAFFGRFLV"
     gene            2305..3441
                     /gene="wecB"
                     /locus_tag="PK849_05810"
     CDS             2305..3441
                     /gene="wecB"
                     /locus_tag="PK849_05810"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPE65677.1"
                     /translation="MEKKIVCVVGTRPEGIKMAPLIRVLRERNFPVTVLSTGQHLQML
                     HQSLEFFGIEADVNLSVMEERQTLDRVTAKVLGGVGAFLDRRPQDLVLVHGDTTTTMA
                     SALAAFYRSIPVGHVEAGLRSGDMARPFPEEANRIITDRLSSLWFAPTEQAAANLKNE
                     GLPVSEGTLAITGNTVIDALKLVLERKHDLCDSLRPLERFPGPLLLMTAHRRESWGEP
                     LESICGAIVDILKQNSEVRILIPLHKNPSVRDVIRRYLADEERAILCEPLDYPDFVWA
                     MQRSSVILSDSGGVQEESAELKKPLLVMRDVSERPEALETGTALLVGTNRVGIAETAL
                     RILSDDVFRKSLLSRGENPFGSGDASEKIADVIAKFFENRQGVA"
     gene            3485..4783
                     /gene="murA"
                     /locus_tag="PK849_05815"
     CDS             3485..4783
                     /gene="murA"
                     /locus_tag="PK849_05815"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPE65678.1"
                     /translation="MESKMKIIGGTQLKGNITAQGAKNAALPVMAAAILLKGKKLKID
                     RVPNLLDVVTMADLLRSLGAVVQFSDHEMEISTPEEISWETPPDLVRKMRASSLVLGP
                     LLARCGKAVMPLPGGCSIGSRPIDLHLKGLTRMGASIELVHGAVHARADGLRGCRIYL
                     DFPSVGATENLMMAAVFAQGETVLENTAREPEIFNLVEALRSMGAFIDADGTGVIRIK
                     GVDDLESASATVIPDRVEAATYILAGAATNGEVTVSDIIPNHIDSLLAKLEEAGALMT
                     IKETEVTIHPSGRLKAVSLKTLPYPGFPTDLQPQVMVALCLAEGTSVVQESVFQSRFL
                     HVSELNKMGAKIDIQSNSAIINGVASLAGADVLATDLRAGAALVLAGLAARDITYVHH
                     LGHISRGYELMEEKLRAIGANISILPSVEDSKGDSPALRS"
     gene            4908..5660
                     /locus_tag="PK849_05820"
     CDS             4908..5660
                     /locus_tag="PK849_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65679.1"
                     /translation="MVAMENEVDYIIDDGLVIAKGRIMVGKSAKAEKNLVRAIRRALF
                     QFPEHRKAVLAFFQKKQPKKLMIIATSISMAEKITRALRLPYPEMIIDITEVASAEEI
                     INARRERHEKGQHVIPASRTQIRKNFAGKLVGHLRGLFKSVDKEDGERTIVRPPFSFF
                     GELTIESSAILDLVRHVLSSNPQITEVKDIRVRYKDDAISMEIVLQVIPGKMDFHRLG
                     VVNQKRVASAVSYFTGMDVDGVDVHIVEVMLP"
BASE COUNT         1335 a         1362 c         1630 g         1504 t
ORIGIN      
        1 cggagtcata ggcacggccg tcgccgcagg cgtcatgctt acgctgctga gctgaaaagc
       61 tacaggaatc tttatccgtt acctgaaggg agactcccgc aatccaggag tctccctttt
      121 tttcgaattc gtctgtgttt gtatatgcct tttttccgac aaatgatatt atataaggat
      181 tgaagaggtc accgggggct cgacgtttct ttctatctcc tggaaattat acgaggaggg
      241 tctgtgcatg aagattgccg taggttctga tcatgctgcg tttgctttga aaaactattt
      301 gcttgagcat cttcggcaaa aaagtggcat cgaaagcgtg gatatgggtg tggatacgga
      361 tgcggtttct tgtgattatc cggatgtggc gttccgtgtt ggagaggccg ttgtttcccg
      421 ccaggcggac agaggccttc ttttgtgcgg taccggtgtc ggaatgagta ttgcagcgaa
      481 caagatccct ggagtgtatg cagcccattg tcatgacgtg gaaactgccc gattcagccg
      541 ccggcataat aatgcgaatg tcctcaccat gggggcgcgg ataatatctc aggaacttgc
      601 ccgcgaaata atcgatgtct ggctcgaaac agattttgaa ggagaccggc atataagaag
      661 attgagtaaa atccttgatt atgaacacaa aatctgtcca agctgtacct tttccgaaaa
      721 aggcaagacg gtcgtctttg aacacccgct tgtgcagcac aaggtaagca tcatccgcga
      781 caaaaatacc tcggtgaaag aattccgtga gcttgtgcag gagattgcag ggctgatggt
      841 ctatgagatt acccgaaatc tgccgctcgt aaaaatagag gtggaaaccc ctctcgcgaa
      901 gacggaagcc tacgcccttg aggggaaaaa actcgcaata gtacccgtcc tcagagcggg
      961 cctcggcatg gtcgacggca ttctccaact cgttccgaac gcaaaagtcg ggcatatcgg
     1021 gctctatcgt gatcccgaga cgcttctccc tgtggagtac tactgcaaac tgcccggcga
     1081 tatcagcgag agagatattc tcgttctcga tccgatgctc gccacgggag gttcggctgc
     1141 cgcagccatc agtctcgtca aagcacgggg cggcaaaaag gtttcactcg tttgcatcat
     1201 agcggctcct gaaggcgtct ccaaggtgca cgccgagcat ccagacgttg atatctacgc
     1261 ggcggctctt gacagtcatc tcaatgacca cggatatatc gttcccgggc ttggcgacgc
     1321 cggagatcgt ctcttcggga cgaagtaagg gactttcgtg gattctctcg ttgcgcctct
     1381 gtcggtttcg ataccgcttc ttttcctctg gggagtgtgc gttacacctc tttcgatcct
     1441 cctctccaat cggtaccgta tagtcgacca gcccggggag cgtaaaattc atacttcagt
     1501 cactccccga ggagccggaa tcgttctctg gctaggattt ctcctatgga gcctctatgc
     1561 agtggaggag gctcctttgc ttcgtttcat ggcggtaggt tctaccctcg tgtttttaag
     1621 cggttactgg gacgatatga aaactttgaa cccggccata cgactcacgt ttcatattgt
     1681 tgccgcaggt cttttcctgc tttttctccc tgtttctctg gttcatatac ctgtgtgcct
     1741 gctctggatt acaggaatga cgaacgcttt caaccttatc gacggagcga acggcctgtg
     1801 cctcatgatg tttatcgtgg catcctcggt ttttgcagcg tgcgggttcc cctttttttt
     1861 ccttccagtc gcgagcttgg ctgctggagt tcttctctgg aattttccca gagcgaaaac
     1921 ctttttaggc gacggcggaa caacgcttct ggggtatctc tactcaagcc tttgtcttta
     1981 ttccttgacc ccgtttctcg aagatgttca tgtgtttgaa ctgccctttt ttctgttgat
     2041 ggcaggcggt attcctgcgg cagatacgct ctttgctata atgagacgat tagcgatgag
     2101 aaaatctccg ttttcacctg atcgcggaca cattcatcat cgtttgctcg acagaggatg
     2161 gagccccgtc cttactgtaa tgattcttag tgttgcacag ttgttagtag tcgccggcgg
     2221 ggtcgcgttt ttcggccgtt ttctcgtgta acagtgtccg gagtgtgtgc ttttaacagg
     2281 atttttgcgt agggatggct gaggatggaa aaaaaaatcg tatgtgtagt cgggacccgt
     2341 cccgaaggga taaaaatggc gcctctgatc cgtgtgcttc gggagcggaa cttcccggtg
     2401 acagtgcttt cgacggggca gcatcttcaa atgctgcatc agtcattgga gttttttgga
     2461 attgaggccg atgttaatct ttccgtgatg gaggaacgtc agacgctcga cagggtgacc
     2521 gccaaagtgc ttggtggggt aggcgctttt cttgatcgtc gaccacaaga cctggtttta
     2581 gtacatggcg ataccacgac aaccatggcc tctgcgctcg cggcctttta ccggagcatt
     2641 ccggtaggcc atgtcgaggc cggcttgcga agcggcgata tggcccgtcc tttcccggag
     2701 gaagcgaaca ggattatcac agacagactg tcttcattat ggttcgctcc aacggagcaa
     2761 gctgcagcga accttaagaa tgaagggctt cctgtatctg aagggactct tgcaatcacg
     2821 gggaatacgg ttattgatgc gctgaagctg gtattggaaa gaaaacatga cctctgcgat
     2881 tctctacgtc cgctagagcg tttcccggga cctctccttc tcatgaccgc gcatcgcaga
     2941 gagtcatggg gggagcctct tgaatcaata tgtggagcta ttgtggatat actgaaacaa
     3001 aacagtgagg tgcggatact tataccgctt cataaaaatc cttcagtacg tgatgttatc
     3061 aggcggtatc tagcggatga agagcgggct attctttgcg aaccactcga ctaccctgat
     3121 tttgtctggg cgatgcaaag aagctccgtt attttgagcg acagcggagg cgtccaggag
     3181 gaatcagcgg aattgaaaaa acctctcctc gtgatgaggg atgtttcgga acgaccggaa
     3241 gctctcgaaa caggtacggc tcttctcgtc ggaaccaatc gggtcgggat agcggagacg
     3301 gcgttacgca ttctctccga cgatgttttc aggaagagtc ttttgagccg gggggagaac
     3361 ccttttggaa gcggcgacgc ttctgagaaa atagctgatg taatagcgaa gttctttgaa
     3421 aacagacagg gtgttgcctg agtctttttg tgtttttcac aatattttcg ggaggaattg
     3481 gaaagtggaa tccaaaatga aaatcatcgg cggtacccag ctgaagggga atataaccgc
     3541 acagggcgct aaaaatgccg cgcttcctgt gatggcggcc gccatactgt taaagggcaa
     3601 aaaattgaag atcgataggg tgcccaatct tctggatgtt gtgaccatgg cagatcttct
     3661 tcgaagttta ggagctgtgg tgcagttcag cgatcacgag atggagatct ccactcctga
     3721 agaaatctca tgggagactc ctccggatct tgttcgcaag atgagggctt cgtcccttgt
     3781 ccttggtcct ttgcttgccc ggtgcggcaa ggccgtgatg ccgttgcccg gcggttgttc
     3841 gatcggcagt cgtcccattg acctccatct taagggcttg acccggatgg gcgcgtcgat
     3901 tgagcttgtc cacggtgcgg tacatgcgcg ggcggatgga ttgagagggt gcagaatcta
     3961 tcttgacttc ccgtcagtgg gagctacaga gaacttgatg atggccgctg tgtttgctca
     4021 aggggaaacc gttctcgaaa atacggcgag agagccggag attttcaacc ttgtagaggc
     4081 gcttcgttcg atgggggctt ttattgatgc cgacggaacc ggtgtgatcc ggatcaaggg
     4141 agtggacgac cttgaaagcg cctccgcgac cgtcattccc gacagggttg aggcagcgac
     4201 atatattctt gccggggcgg ctacaaatgg agaggtgacg gtttccgaca tcataccgaa
     4261 ccatatcgat tcacttcttg ctaagcttga agaggcgggc gccttgatga caatcaagga
     4321 aacggaggtg acaatacacc cttcgggaag gctgaaggca gtctctttga aaacccttcc
     4381 ctatcccggt ttcccgacag atcttcagcc tcaggttatg gttgcattgt gtcttgcgga
     4441 aggaacgagc gttgtccagg aaagcgtttt tcagtccagg ttcctccacg ttagcgagct
     4501 caacaagatg ggggccaaaa tcgacattca gagcaactcc gctattatca acggcgtagc
     4561 atcccttgca ggagccgatg tcctcgcgac ggacctgagg gcgggggctg ctcttgtact
     4621 ggcggggctt gcagcaagag atattaccta tgttcatcat ctcgggcata tttcacgcgg
     4681 gtatgaactt atggaagaaa agttgcgcgc gatcggagca aatatttcga ttctcccatc
     4741 cgtagaggat tccaaagggg attctccggc gcttcgttct taaagccggc cggaatgtct
     4801 cttttcctgc tctatggtat gtcgtcatgg cgttaaacga ttccggtgtg acaagagtta
     4861 ttgcttttgt tggtcctgca ggtaccggta agagtcaacg ggcgcagatg gtcgccatgg
     4921 aaaacgaggt tgactatata atcgacgacg ggcttgttat tgccaaggga cgtataatgg
     4981 tgggaaagag tgcaaaggct gagaagaacc ttgtgcgtgc aatacgaaga gctctttttc
     5041 agttcccgga gcataggaaa gcggtattgg cctttttcca gaagaaacaa ccgaagaagc
     5101 tgatgattat tgcaacgtcg atttcgatgg cggagaagat caccagagcg cttcgtcttc
     5161 cgtatccgga aatgatcatc gatataacgg aggtagcttc ggcagaagaa atcatcaatg
     5221 ccagaagaga gcgtcatgaa aaaggacagc acgttattcc cgcctccagg acgcagatac
     5281 ggaaaaattt cgccggaaaa ctagttggac atctgcgggg actctttaaa agcgtcgaca
     5341 aggaggacgg agaacgaaca attgttcgcc ctccattcag tttttttgga gaattgacta
     5401 tagagtcgtc ggctatcctt gatcttgtcc gccatgtgct ctcttcgaat ccgcagatta
     5461 cggaagtgaa ggatattcgt gttcgatata aagatgacgc tatttctatg gagattgtac
     5521 ttcaggttat tccggggaaa atggatttcc accgacttgg tgtagtcaat caaaagagag
     5581 tagcatccgc agtcagctac tttacgggaa tggatgtgga cggtgttgac gtccacatag
     5641 tggaggtaat gctgccatga gcgtctttgc gagcggagcc gacagcgggt tggcatggga
     5701 ggaactgaaa aggcgtgtag aaacctgttc atcgtgcggc ctgtgttcga cgcgaaagaa
     5761 gacggttttt ggtcagggaa gcgagacctc cccggttgtt ttcgtcggcg aaggtcccgg
     5821 agcggacgag g
//
LOCUS       DAOPKU010000143         5821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14800_length_5821_cov_4.565210, whole genome shotgun sequence.
ACCESSION   DAOPKU010000143 DAOPKU010000000
VERSION     DAOPKU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14800_length_5821_cov_4.565210"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK849_05825"
     CDS             <1..194
                     /locus_tag="PK849_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65680.1"
                     /translation="AVHKPEGLLVHRSPLDRLAFPHPFTGERLDIFCPPEESFLAALR
                     ALGGEEAAGLAGTPGLRGE"
     gene            complement(272..1276)
                     /gene="asrC"
                     /locus_tag="PK849_05830"
     CDS             complement(272..1276)
                     /gene="asrC"
                     /locus_tag="PK849_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HPE65681.1"
                     /translation="MTLDLNRKKVTKNAFRITKVRGATAIRVRVPGGHLDAELMKTIA
                     QIAERFGDGTLHITIRQGFEIPNIPFERMPEVNAALAPIIERLELPHGVKIESPSDGY
                     PAAGMRNISACIGARVCPFANTDTTALAQKLEALVYPNDFHVKIAVTGCPNDCVKAHM
                     QDFGIIATCRPEYDYDQCIGCEACVDACRQKVTGALRMQDGKVVKDDRRCIGCGECVL
                     ACPVAAWTRPPVTFYRLLIMGRTGKKNPRLAMPFLDRVTEEVVLKVIKNTLDYVDKHI
                     LRSLVKEHIGYIVDRTGYPVFRDAVLEGVQLNPEARIAKTMHWPGYYTLDDGNMKQAE
                     "
     gene            complement(1290..2084)
                     /gene="asrB"
                     /locus_tag="PK849_05835"
     CDS             complement(1290..2084)
                     /gene="asrB"
                     /locus_tag="PK849_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HPE65682.1"
                     /translation="MKENPYRPFPAKITGIKAETDCEFTLTVAASVAMTPGQFFQVSL
                     PKIGEAPISISGFGPDWIQFTVRAVGRLTNALQELRTGENLFIRGPYGTGFPLDTFRG
                     SNLAIVAGGSGTAPVRPLIRGALDGSLPVRSLRVIAGFKSKESLLFANEFDEWRTKGE
                     VLVTIDKPQEGWDGPTGLVTEHIRAMKLDDPADVQFVVVGPPVMMKFAVAEVLLLGVP
                     EDNIWVSFERLMSCGLGKCGHCKIDSAYICLDGPVFRFSRAAGLID"
     gene            complement(2081..3103)
                     /gene="asrA"
                     /locus_tag="PK849_05840"
     CDS             complement(2081..3103)
                     /gene="asrA"
                     /locus_tag="PK849_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HPE65683.1"
                     /translation="MSSFSFSLEEFSRVFDALGERYALSGPKRFPGKGRFTGTDLVKY
                     APLVSFDELELDEKSSMSPKSLVFPPDETTFYFNGDEFTEAPLEDERETVVFLRPCDI
                     NGFRRLDAIFLENGGTKDPYYLRRREKLHFFMIECATSFENCFCVSMGSNVAGEYAVA
                     LRFGQDSVLVQVKEELFASLFASGAPSEFTPEFVRENARTVEVPDVDEMPPELFLHPL
                     WDEYDARCIACGRCNTSCVTCTCFSTRDIFYADSRRSGERRRVWDGCHLDGFTDMAGG
                     HSFRKKKGERMRFKTFHKIYDFKKRFGYNMCVGCGRCDDVCPEYISFAECINKVAAAL
                     KGGDAK"
     gene            complement(3106..3594)
                     /locus_tag="PK849_05845"
     CDS             complement(3106..3594)
                     /locus_tag="PK849_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPE65684.1"
                     /translation="MTFALSAADVFEAAILLEERGARFYADAAARCTGEAKKLLSGLS
                     SMEKNHAERFRSILEELRASSSAPEPRPEEAAQYLEALTSDRIFTETISIGAKESYPE
                     ILEKAIVVEKNSVFFYTAVKDILSSKMEAKDVDRLIAEEIGHFHALTDALRIRRERNG
                     GA"
     gene            complement(3827..4174)
                     /locus_tag="PK849_05850"
     CDS             complement(3827..4174)
                     /locus_tag="PK849_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE65685.1"
                     /translation="MIITRKYEEQKIAETAHRVDARGMYETEDAAISIIALQPGESLK
                     RHITPVDVAFFVLDGKGTVEIGDEKQEVGKYTLVESPKDILHCWYNTGDELLRFMVIK
                     APKPTKKTIFVGA"
     gene            complement(4190..4909)
                     /locus_tag="PK849_05855"
     CDS             complement(4190..4909)
                     /locus_tag="PK849_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65686.1"
                     /translation="MSGIRIEALRKRYGSGETAVDALKGVDMEVAPGEVVGLIGPSGS
                     GKSTLLKCLGAVIEPTGGKMVLGGKTIYEDGWKISDLRMLRRDSIGFIFQAPYLIPFL
                     NALDNVALLPMLAGHPDDESRERAMELLEALDVAHRAAAMPSQLSGGEQQRVSIARAL
                     ANHPPVILADEPTAPLDSERALSVVRILHSMAHQYETAIIVVTHDEKIIPTFRRIYRI
                     RDGKTVEEAGEGRSTAEEITE"
     gene            complement(4919..>5821)
                     /locus_tag="PK849_05860"
     CDS             complement(4919..>5821)
                     /locus_tag="PK849_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE65687.1"
                     /translation="RRGGEDTRVMVAGVEPGEPGAPGQPGYLTAGRHLARSHYEAVAD
                     EASGFRLGDTIRIRRNSYTVVGLTRRMVSSGGDPMVFIPLKDAQEAQFLKDNDAILQQ
                     RRRTAANPSLNRPGVPGMLDGVIASQGVNPYVNAVLVRLLPGADPEKTAEHMRRWMRL
                     EVYTRAQMEEILLGKLIATSSKQIAMLLVILAVVSAAIVAFIIYTLTLGKIREIAVLK
                     LIGTKNRTIIGMIMQQAIALGVLGFMIGKVSATFWAPKFPKYVLLEVRDAATGFAAVI
                     IMCVIASLLAIRVAVKVDPAEAIG"
BASE COUNT          994 a         1894 c         1770 g         1163 t
ORIGIN      
        1 tcgcggtgca caagccggag gggctgctgg tgcaccgctc gccgctcgac cggctcgcct
       61 tcccgcaccc cttcacgggg gaacgtctcg acatcttctg ccctccggag gagagctttc
      121 tcgccgctct gcgagcgctg ggcggcgaag aggccgccgg tcttgccggc acccccggcc
      181 tccggggaga ataaaaagag gagcactccg gccggaaccg gcgctcccct cttttgtgtg
      241 gttatagact ccgcgcggaa tcgtacgacc gctactccgc ctgcttcata ttcccgtcgt
      301 ccagcgtata gtaccccggc cagtgcatcg tcttggcgat ccgcgcctcg gggttcagtt
      361 gcactccttc gagcaccgcg tcgcggaaga cggggtaccc cgtcctgtcc acgatgtagc
      421 cgatgtgctc cttgacgagg ctccgcagga tgtgtttgtc cacgtagtcg agggtgtttt
      481 tgatcacctt gaggacgacc tcctccgtca ccctgtccag gaagggcatg gcgaggcgcg
      541 gattcttctt tccggtgcgc cccatgataa gcagccggta gaacgtgacg ggggggcgcg
      601 tccacgcggc cacggggcag gccaggacgc actcgccgca gccgatgcac cgccggtcgt
      661 ccttgaccac cttgccgtcc tgcatcctga gcgctcccgt caccttctgc ctgcacgcgt
      721 cgacgcacgc ctcgcagccg atgcactggt cgtagtcgta ctcgggcctg cacgtggcga
      781 tgatgccgaa gtcctgcatg tgcgccttga cgcagtcgtt ggggcagcct gtaacggcga
      841 tcttgacgtg gaagtcgttg gggtagacga gcgcttcgag cttctgcgcg agggcagtcg
      901 tgtcggtgtt ggcgaagggg cagacgcgcg cgccgatgca cgcggagatg ttccgcatcc
      961 ccgccgcggg gtagccgtcc gacggcgact cgatcttcac cccgtgcggc agctcaaggc
     1021 gttcgatgat cggcgcgagc gccgcgttca cctcgggcat ccgttcgaag gggatgttcg
     1081 gaatctcgaa gccctgccgg atggtgatgt ggagcgtgcc gtccccgaac cgctccgcga
     1141 tctgcgcgat ggtcttcatg agttcggcgt cgaggtgtcc gcccggtacg cggacgcgga
     1201 tcgccgttgc gccgcgcacc ttcgtgatgc ggaaagcgtt cttcgtgact ttctttctgt
     1261 tgaggtcgag ggtcatcgtg cgccacctct cagtcgatga gcccggcggc tcgggagaag
     1321 cggaataccg gcccgtccag gcagatgtag gcggagtcta tcttgcagtg cccgcacttt
     1381 ccaaggccgc atgacatgag gcgctcgaag gagacccata tattgtcctc aggcacgccc
     1441 agcagcagga cctccgcaac agcgaacttc atcatcacgg gcggccccac gacgacgaac
     1501 tgcacgtcgg cggggtcgtc gagcttcatc gcgcggatat gctcggtgac cagccccgtc
     1561 gggccgtccc acccctcctg cggtttgtcg atcgtgacga ggacctcgcc cttcgtgcgc
     1621 cattcgtcga actcgttggc gaagagcagg ctctccttcg acttgaatcc cgcgatcacg
     1681 cgcagagagc gcacgggaag cgagccgtcg agcgcgcccc ggatgagcgg acgaaccggc
     1741 gccgtgccgg aacctccggc cacgatcgcc aggttgctcc cgcggaacgt atccagcggg
     1801 aagcccgttc cgtaggggcc tctgatgaag aggttctctc cggtacgaag ctcctgcagg
     1861 gcgttcgtga gccggccgac ggcgcggaca gtaaactgga tccagtccgg tccgaagccg
     1921 ctgatcgaga tgggggcctc gccgatcttc ggcagggaga cctggaagaa ctgtccgggc
     1981 gtcattgcga cggaagcggc gaccgtcagc gtgaactcgc agtccgtctc cgccttgatc
     2041 ccggtgattt tggccgggaa ggggcggtag ggattctcct tcatttcgcg tcccctcctt
     2101 tcagggcggc ggctaccttg ttgatacact cggcgaacga gatgtactcc ggacagacgt
     2161 cgtcgcacct gccgcacccg acgcacatgt tgtagccgaa gcgttttttg aagtcgtata
     2221 tcttatggaa ggttttgaag cgcatccgct cccccttctt cttcctgaag gagtgccccc
     2281 cggccatgtc ggtgaaccca tcgagatggc agccgtccca gacgcggcgg cgctcgccgg
     2341 agcggcggct gtccgcgtag aagatgtccc gcgttgaaaa gcaggtgcag gtgacgcagg
     2401 aggtgttgca cctgccgcag gcgatgcagc gcgcgtcgta ctcgtcccac agcgggtgca
     2461 ggaagagttc cgggggcatt tcgtcgacgt ccgggacttc gacggttcgt gcgttctcac
     2521 ggacgaactc gggtgtaaat tcggaaggcg ccccggaggc gaagaggctg gcgaagagtt
     2581 cctccttcac ctggacgagg accgaatcct gtccgaaacg cagcgcgacg gcgtactccc
     2641 cggcgacgtt ggagcccatg gagacgcaga agcagttctc gaacgaggtt gcgcattcga
     2701 tcatgaagaa atgaagcttc tccctgcggc gcaggtagta ggggtccttg gttccgccgt
     2761 tctccaggaa gatggcgtcg agcctccgga agccgttgat atcgcagggg cgcagaaaga
     2821 cgacggtctc ccgctcgtcc tcgagcggag cctcggtaaa ttcgtcgccg ttgaagtaga
     2881 acgtcgtctc gtcgggcggg aagacgagcg acttgggcga catgctactc ttctcgtcca
     2941 gttccagctc gtcgaaagag acgagcggag cgtatttcac caggtccgtg ccggtaaagc
     3001 gccccttgcc gggaaaacgc ttcggcccgg agagggcgta ccgctcgccc agcgcgtcga
     3061 aaacgcggga gaactcctcc agcgagaagg agaaggagga catcgctacg cccccccgtt
     3121 ccgctcccgc cggatgcgga gcgcgtccgt gagcgcatgg aagtgcccta tctcctcggc
     3181 gatgaggcgg tcgacgtcct tcgcctccat cttggacgag aggatatcct tcaccgcggt
     3241 gtagaagaag acggagttct tctcgacgac gatcgctttt tcgaggatct cagggtagct
     3301 ctcttttgcc ccgatgctga tcgtttctgt gaatatcctg tcggaggtca gcgcctcaag
     3361 atactgtgcc gcctcttccg gccggggctc cggagcggaa gacgacgcgc ggagctcctc
     3421 caggatactc cggaagcgtt ccgcatggtt cttctccatg gacgacaacc ccgagagcag
     3481 cttcttcgcc tcgccggtac agcgtgcagc cgcatcggcg tagaagcgcg ctcctctctc
     3541 ctccagcagg atcgcggcct cgaagacatc cgccgctgaa agcgcaaaag tcacaacaat
     3601 ctcccctttc gtctccggtt ctctctccgg cggaccgccg gtatttaaga cgcttgcaca
     3661 ctttcgctcc actctttaca ggtattatgg tactttaatt cctgaatatg tcaagacgcc
     3721 tccatcttga tcggaaacgg tggaactccc gttcctgctt ttattataca gcgagcgcgc
     3781 cgttccccaa ggacgcggcg cgctcgcttc cggaacagcc tctccgtcag gcgccgacga
     3841 agattgtttt tttagtcggt ttcggcgctt tgatcaccat gaaccgcagc agttcatcgc
     3901 cggtgttgta ccagcagtgc aaaatatcct tcgggctttc gacgagcgtg tactttccca
     3961 cttcctgctt ctcgtcgccg atctcgactg ttcccttccc gtcgaggacg aaaaacgcga
     4021 cgtcgaccgg cgtgatgtgc cgcttcagcg actcccccgg ttgcagcgcg atgattgaaa
     4081 tcgccgcgtc ctcggtctcg tacatgcccc gcgcgtccac cctgtgcgcg gtctccgcta
     4141 tcttctgctc ttcatatttc cttgtgataa tcatagtatt cttcctcctt cactctgtta
     4201 tttcttctgc cgtcgaccgg ccctcgccgg cctcttccac cgtctttccg tcgcggatcc
     4261 tgtagatcct gcggaaagtc ggaataatct tctcgtcgtg cgtcactaca atgatcgccg
     4321 tctcgtactg gtgcgccatc gagtgcagaa tccggaccac ggagagagcg cgctcgctgt
     4381 ccagaggggc ggtgggctcg tcggccagta tgaccggcgg atgattcgcc agcgcccggg
     4441 cgatcgagac gcgctgctgc tctcctccgg aaagttgcga cggcatggcc gccgcccggt
     4501 gcgcgacgtc gagggcctcc agaagctcca tggcccgctc ccgcgattcg tcgtcgggat
     4561 gaccggcgag catcgggagc aacgcgacgt tgtccagcgc attgagaaaa gggataaggt
     4621 agggcgcctg aaagataaac cctatactgt cccgccgcag catccggagg tcggagatct
     4681 tccatccgtc ctcgtagatc gtcttgccgc cgagcaccat ttttcctccc gtcggctcga
     4741 tcacggcgcc gaggcactta agcagcgtac tcttccccga tcccgaaggc ccgatgagcc
     4801 cgacgacctc tccgggagca acctccatgt ccactccctt gagcgcgtcc accgccgtct
     4861 ctcccgagcc gtaccgcttc ctgagcgctt cgatgcgtat cccgctcata ggggccgcct
     4921 accctatcgc ttccgcaggg tccaccttga cggcgacccg tatcgcgaga agacttgcga
     4981 tcacgcacat tatgatcacg gcggcgaatc ccgtcgccgc gtcgcggacc tccagcagca
     5041 cgtatttcgg gaacttcggc gcccagaagg tcgccgagac cttgccgatc atgaagccga
     5101 ggacgccgag ggcgatagcc tgctgcatga tcatgccgat aatcgtccgg ttcttcgtgc
     5161 cgatgagttt gagtaccgcg atctccctga tcttccccag ggtgagcgta tagatgatga
     5221 aggcgacgat ggcggcgctc acaacggcga gtatcacaag aagcatagcg atctgcttcg
     5281 acgacgtggc gatcagtttc cccagcagaa tctcttccat ctgcgccctg gtgtagactt
     5341 cgaggcgcat ccaccgccgc atgtgttccg ccgttttctc gggatcggcg cccggaagga
     5401 ggcgcaccag aacggcgttg acatacgggt tgacgccctg agatgcgatc acgccgtcga
     5461 gcattccggg aacccccggc cggttgagcg acggattcgc cgccgtccgc cgccgctgct
     5521 gcaaaatagc gtcgttgtcc ttgaggaatt gcgcctcctg cgcgtctttc agcgggatga
     5581 agaccatcgg gtcgcctccg gacgagacca tccgccgcgt gagtccgacc acggtgtagc
     5641 tgttcctgcg gatgcggatc gtatccccga gacggaagcc cgacgcctcg tcggccacgg
     5701 cctcgtagtg actgcgggcg agatgtctcc ccgccgtaag gtaccccggc tgccccggag
     5761 cgccgggttc ccccggctcg acccccgcga ccatcacccg ggtatcctcg cctccacgcc
     5821 g
//
LOCUS       DAOPKU010000144         5797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14884_length_5797_cov_4.847440, whole genome shotgun sequence.
ACCESSION   DAOPKU010000144 DAOPKU010000000
VERSION     DAOPKU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14884_length_5797_cov_4.847440"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PK849_05865"
     CDS             complement(<1..738)
                     /locus_tag="PK849_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE65688.1"
                     /translation="MLLIGNGKVITRDENNTVIENGAVVIDGAHIKAVGKTEDLKKAY
                     PGATFKDAKGRLVMPGLINVHMHYYSTFARGIALDSPPATKFSEILEGLWWRLDKCLT
                     LEDVYYSVMGPGIDQIKCGVTSVIDHHASPFAVRGSLAKIAEASAELGLRSNLCYEVS
                     DRDGEQSRVDGITENIEFIKACAAKKDDMLKGMFGLHASMTISDKTLNESVEQMAGLD
                     AGFHVHVAEGIEDLEDSKAKYGLGVVER"
     gene            complement(751..3765)
                     /gene="ygfK"
                     /locus_tag="PK849_05870"
     CDS             complement(751..3765)
                     /gene="ygfK"
                     /locus_tag="PK849_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HPE65689.1"
                     /translation="MSDIMRMIPFGNLMDWMLVEHGKEGSIFGIRKNKFYKNATGKQI
                     VVCGESISSPIGPAAGPNSQLAQNIIAAYLAGSRFIELKTVQIMDGEELRKAVAKPCI
                     NAQDEGYNVEWSTELTVQEAFDEYVKAWFAIQVMAKELGISAQRDFAYNMSVGYDLKG
                     IQSPKIDGFIEGLKNASGSGIWKTCSQWLRDNLSKFSNVNASDVDAIESRLCSSITLS
                     TLHGCPPEEIERISRYLFEEKGLHVFVKCNPTLLGYETARDLLDRMGYTYIDFTDHHF
                     KHDLQYSDGVAMFKRLQEFAKERGLEFGLKLTNTFPVNIKNEELPGEQMYMSGRSLFP
                     LTITLASKLSREFGGKLQISYSGGADFFNIDSILNVGIQPISMATTILKPGGYERMKQ
                     IAEKAEPYLNGAFRGVDVKALDALAASVFEKRFHKKSARPVGSRKTESRLPLFDCAKA
                     PCQDGGCPINQQVPTYLRLVNEKKYAEAFDVIAMDNSSPAVTGTICDHQCQHVCTRLD
                     YEQPLEIREMKKIAVLNAQDEYIARMQPAKIVSKKKAVVVGAGPAGVGTAFFLRRNGI
                     ETTVLERRAEPYGIVKYVIPEFRIGEDMIARDVALAKKAGVQFVFGVDGKIDIEALKK
                     EYDYVVLATGAWKPGESPVKEGRELVRDALSFLEEYKKKKGHVEIGGTVAIIGGGDVA
                     MDCARAAKRAPGVKRSIVVYRRTVAYMPAEPEEKELALHDGVELMELLAPVKYDGKTL
                     LCEKMQLGEKDASGRRGVTGTGEMVPVAADTVIGATGAKVDSEIFGANGIALDAKGRV
                     VMNEENETSVSNVYVAGDCKAGPATVVKGIADAKTVTKSILTKEGLSPDFAVTELVQD
                     LASLYEKKGVLKEAEKDQNDGARCLACDVVCELCCDVCPNRANVLVVVDGKHQIVHLD
                     GMCNECGNCGIFCPHTGDPYKDKVTLFWTEEDFKESTNKGFFRVEGDRYSVRVEDGSV
                     VSWRRGEPGVSLEMAKTLGAVLERYPYYMMNL"
     gene            complement(3802..>5797)
                     /locus_tag="PK849_05875"
     CDS             complement(3802..>5797)
                     /locus_tag="PK849_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE65690.1"
                     /translation="AGTLSEARAAAAAVKVEYAPLPTLFDPEKAMDKDSPSLHGEDGA
                     YAPLPVPFRAEENVAGEVLFSFGDMEKGFADADFIVDETYRTHYTAHCALEPHAASAF
                     FDEKGRLVIVASTQVPFHARRTVSRVVGIPAHMIRVIKPRIGGGFGSKQEIILELYAA
                     LAAWKYKRNVKAEMSRSEVFTAARTRHPMRVRIKTGVKNDGSITALDMSDVMNTGAYG
                     AHALTVLSNAGSKVLPMFNKIENMNFVGRAVYTNLPIGGAYRGYGATQGYFAFNQHMD
                     GIARRLGVDFLDFVKKHHIREGETSEVFKAIGEGTEGVSQIIRSCKLSECIDKGAEAI
                     GWSNLRGKKIANGPVVKGIGAAVSMQGSGIPQVDMASASMKMNDDGSFNLYVGATDIG
                     TGSDTILSQIAAETLNVPLEMILILSSDTDLTPFDTGAYASSTTYVSGQAVLRCAEKV
                     RSQILSVAEKMTGAAPESLVFKESRVVNTATGEETSFSDIACFAMYSHDQFQIQASAS
                     FTGSESPPPFMAQFAEVDVDTETGKVRVVKFVSVGDCGQPLNPKLVEGQIEGATMNGI
                     SYALTEQYIFNSKGKMTNNSFWDYKLFGTLDMPEMKTIVVDSDEPTGPYGAKSISEIG
                     INGPAPAIANAIYDAVGVRIYDLPLTPEKVLKAIRARTDG"
BASE COUNT         1130 a         1777 c         1537 g         1353 t
ORIGIN      
        1 gcgctcgacc acgcccagcc catacttcgc cttgctgtcc tcgaggtctt caatgccttc
       61 ggcgacgtgc acgtggaatc ccgcgtcaag ccccgccatc tgttccacgc tttcattcag
      121 cgtcttgtcc gagatggtca tggacgcgtg cagtccgaac atgcccttga gcatgtcgtc
      181 cttcttcgcc gcgcacgcct tgatgaattc gatattctcg gtaatgccgt ccaccctgct
      241 ctgctctccg tcgcggtcgg agacttcgta gcagagatta ctccgaaggc ccagctccgc
      301 cgaagcttcg gcgatcttcg caagactgcc gcgaaccgcg aacgggctcg cgtgatgatc
      361 aatgaccgac gtgacgccgc acttgatctg gtcgattccc ggccccatga cgctgtagta
      421 gacgtcttcg agcgtaaggc acttgtcgag cctccaccag agcccttcaa gaatttcgga
      481 gaatttcgtc gcaggaggac tgtcgagcgc gatgcctctc gcgaacgtgc tgtaatagtg
      541 catgtggacg ttgataaggc cgggcatcac aagccgccct ttcgcgtcct tgaacgtcgc
      601 gccggggtag gccttcttca gatcctcggt cttccccact gcctttatat gcgcgccgtc
      661 gatcacgacc gcgccgttct ctatgactgt gttgttctcg tctcttgtga tgacttttcc
      721 gttgccgatc aaaagcatgc cgtgcacctc ctagagattc atcatgtagt aggggtagcg
      781 ctcgagcact gctccgagcg tcttcgccat ttcaagggag acgcccggtt cgccgcggcg
      841 ccaggagacg accgaaccgt cttcgacacg cacagagtac cggtcgcctt cgacgcggaa
      901 gaatcccttg tttgtactct ccttgaaatc ctcctcggtc cagaagagcg tgaccttgtc
      961 cttgtaggga tctccggtat gcgggcagaa aatgccgcag tttccgcact cgttgcacat
     1021 accgtcaaga tgcacgatct ggtgttttcc gtccacaacc acgaggacat tggccctgtt
     1081 ggggcagacg tcgcagcaga gttcgcagac gacatcgcat gcgaggcagc gggcgccgtc
     1141 gttctgatct ttctcggctt ctttcaagac gccttttttc tcgtacaacg acgccagatc
     1201 ctgcacaagc tccgtgaccg cgaaatcggg ggagagcccc tcctttgtaa gaatgctctt
     1261 cgtcacggtt ttcgcgtcgg ctatcccctt gaccaccgtt gccggtcctg ccttgcagtc
     1321 acctgcgacg tagacgttcg aaacggaggt ttcgttttct tcgttcatta caacgcgccc
     1381 cttggcgtcg agcgcgatgc cgttggctcc gaagatttcc gagtctacct tcgcgccggt
     1441 cgcgccgatg accgtgtccg ccgccaccgg gaccatttcg cccgtaccgg tcacgccgcg
     1501 ccgccccgag gcgtccttct cgccgagctg catcttttcg cagaggagcg tcttcccgtc
     1561 gtacttcaca ggggcaagca gctccatgag ctccaccccg tcgtgcagcg ccagttcctt
     1621 ctcttccggt tcggcaggca tataggcaac ggtgcggcgg tagacgacga tcgaccgctt
     1681 cactccgggg gcgcgcttcg ccgccctggc gcagtccatt gcgacatcgc cgccgccgat
     1741 gatcgcgaca gtgccgccga tctcgacatg ccccttcttc tttttatact cttcaaggaa
     1801 ggaaagcgca tcgcgaacca gctcccgccc ctctttcacc ggagactcgc cgggtttcca
     1861 agctcccgtc gcaagcacga catagtcgta ctccttcttc agcgcttcta tatctatttt
     1921 tccgtcgacc ccgaagacga actgtactcc agccttcttc gcgagagcga cgtcgcgggc
     1981 aatcatgtcc tcgcctatac ggaactctgg aatgacatac ttcacgatgc cgtacggttc
     2041 ggcccggcgc tcaagcaccg tcgtctcgat gccgttccgc cgaaggaaaa acgctgtccc
     2101 cactccggca ggaccggcgc cgacaacaac cgcctttttc ttcgagacga tttttgcagg
     2161 ttgcatccgg gcaatatact catcttgcgc gttgagcacg gcaatcttct tcatctcgcg
     2221 tatctcaagc ggctgttcgt agtcgagccg ggtacagacg tgctggcact gatggtcgca
     2281 gattgttccc gtgactgccg gcgaactgtt gtccatcgcg atgacatcga acgcttcggc
     2341 gtacttcttc tcgttgacca gacggagata cgtcggaacc tgctggttaa tggggcaccc
     2401 gccgtcctgg cagggggcct tggcgcagtc gaaaagcgga agacgcgatt cggtcttcct
     2461 tgatccaaca gggcgggcgc tttttttgtg gaagcgcttt tcaaagacgc tcgccgcaag
     2521 ggcgtcaaga gccttcacgt ccactccgcg gaacgccccg ttcaggtacg gctccgcttt
     2581 ctcggctatc tgcttcattc tttcgtatcc gccgggcttg agaattgtag tggccatgct
     2641 gatcggctga ataccgacat tcagtatgga gtcgatatta aagaagtcgg cgccgcctga
     2701 gtacgagatc tggagctttc cgccgaattc gcggctcagc ttgctcgcca aggtaatcgt
     2761 cagggggaaa agcgaacggc cggacatgta catctgttca ccgggcagct cttcattttt
     2821 aatattcacg gggaaggtgt tggtaagctt cagaccgaac tcgagtcccc gctccttcgc
     2881 gaactcctga agcctcttga acatcgcaac gccgtcgctg tactgaaggt cgtgcttgaa
     2941 gtgatggtcc gtgaagtcaa tataggtata ccccatccgg tcaagaagat ctcgggccgt
     3001 ttcatacccc agaagcgtcg gattgcactt cacaaagacg tggagcccct tctcctcgaa
     3061 cagataccgt gaaatacgtt cgatctcctc cggcgggcag ccgtgaagcg tagaaagcgt
     3121 tatcgacgaa cataaccggc tttcgatagc atccacatcc gaagcattga cgttgctgaa
     3181 cttcgaaagg ttgtcccgca gccactggga acacgtcttc cagatgccgc tcccggaggc
     3241 gttcttcaac ccttcgatga acccgtcgat cttgggggac tgaattccct tcaaatcgta
     3301 gccgacgctc atattgtagg cgaaatcgcg ttgtgcgctg attccaagct cctttgccat
     3361 cacctggatt gcgaaccagg ccttgacata ttcgtcgaac gcttcctgga cagtgagctc
     3421 cgtagaccat tccacgttgt acccttcgtc ctgagcgttg atacagggct tggctaccgc
     3481 cttccgaagt tcttctccgt ccatgatctg cacggttttc agctcgataa agcgtgaacc
     3541 ggccagatag gctgcaatga tattctgcgc aagctgggag ttaggaccgg ctgcaggacc
     3601 gatcggcgag ctgatggatt cgccgcagac cacgatctgc tttcccgtag cgttcttata
     3661 gaatttgttt ttacggatac cgaagatgga gccttccttg ccgtgttcga ccagcatcca
     3721 gtccatcagg tttccgaaag gaatcattct cataatgtcg ctcattcctg cacctccgca
     3781 atcataatcg ctggattttg cttacccatc cgtacgcgcg cgaatcgcct tgagcacctt
     3841 ctcgggcgtc aggggaagat cgtaaatccg gacgccgacg gcgtcataaa tcgcgttcgc
     3901 gatagccgga gccgggccgt tgataccgat ttcagaaatg gacttcgccc cgtacggccc
     3961 cgtcggctcg tcggaatcga ccacaatggt cttcatctca ggcatatcca gcgtgccgaa
     4021 cagtttgtaa tcccagaagg agttattggt catcttcccc ttcgagttga aaatgtactg
     4081 ttcagtcagc gcgtagctga taccgttcat tgtggcgccc tcgatctgcc cttcgacaag
     4141 cttggggttg agcggctggc cgcagtctcc cacggacacg aacttgacaa cgcgcacctt
     4201 tcctgtttcg gtatcgacgt cgacctcggc gaactgcgcc atgaaaggag ggggcgactc
     4261 cgagcctgtg aacgacgccg aagcctgaat ctggaactgg tcatgactgt acatggcaaa
     4321 gcaggcaata tcggagaagg acgtctcttc gcctgttgca gtattgacca cgcggctctc
     4381 cttgaataca agactctcgg gagcagcgcc cgtcatcttt tccgcaacgg agagaatctg
     4441 cgaacgcacc ttctctgcgc atcggaggac cgcctgcccg gagacgtacg tggtggacga
     4501 agcgtaggct ccggtatcga acggcgtcag atccgtatcg gaggagagaa tcaggatcat
     4561 ttcaagcggc acgttcaaag tttccgccgc gatctgggag agaatcgtat ccgaacccgt
     4621 gccgatatcg gtggcgccga cgtagaggtt gaacgaaccg tcgtcgttca tcttcatcga
     4681 ggcgcttgcc atatcaacct gaggaatgcc cgagccctgc atggatactg ccgcgccgat
     4741 tcccttcacc acgggaccgt ttgctatctt tttgccgcga agattgctcc agccgatcgc
     4801 ttccgctccc ttgtcgatgc actcgctcaa cttacacgac cggattatct ggcttacacc
     4861 ttctgtgcct tcgccgatgg ccttgaagac ttcggatgtt tcgccttccc ggatgtggtg
     4921 cttcttaacg aaatcgagaa aatcaacgcc gagcctccgg gcgattccat ccatgtgctg
     4981 gttgaaggcg aagtagccct gggttgcccc gtagccgcga taggcgcctc cgatgggaag
     5041 attcgtgtag accgctcttc ccacgaaatt catattttcg attttattga acatcgggag
     5101 caccttggaa ccggcgtttg agagcaccgt cagcgcatgc gctccgtatg ccccggtgtt
     5161 catcacgtcg ctcatatcga gcgcggtgat cgatccgtcg ttctttacgc cggtcttgat
     5221 gcgaacgcgc ataggatgcc tcgtcctcgc cgctgtaaaa acttcggaac ggctcatctc
     5281 cgctttgacg ttccgtttgt acttccacgc cgccagcgct gcatagagct caagaatgat
     5341 ctcctgcttg ctgccgaagc ccccgccgat tctgggtttt atcacgcgga tcatatgcgc
     5401 gggaatacct acgaccctgc tcacggtccg tctggcatga aaaggcacct gcgtagaagc
     5461 cacaatgaca agacgtccct tttcgtcgaa gaacgccgat gccgcatgcg gctccagcgc
     5521 acaatgcgcc gtatagtgcg tcctgtatgt ttcgtcgacg ataaaatccg catccgcaaa
     5581 ccccttctcc atatcgccga acgagaagag tacctctccg gcaacatttt cctccgcacg
     5641 gaaagggacg gggagcggcg cgtacgctcc gtcttcaccg tgaaggcttg gagaatcctt
     5701 atccatagcc ttttccggat cgaagagcgt cggaagggga gcatactcca cttttaccgc
     5761 agcggcggca gcccgcgctt cggaaagggt tccggcg
//
LOCUS       DAOPKU010000145         5791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_14912_length_5791_cov_4.287308, whole genome shotgun sequence.
ACCESSION   DAOPKU010000145 DAOPKU010000000
VERSION     DAOPKU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14912_length_5791_cov_4.287308"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /gene="hypF"
                     /locus_tag="PK849_05880"
     CDS             <1..1929
                     /gene="hypF"
                     /locus_tag="PK849_05880"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HPE65691.1"
                     /translation="YRYPFINCTDCGPRYTIIRELPYDRPKTTMASFVMCPKCSSEYA
                     DLEHRRFHAEPNACPLCGPEVWLTDSNGTELTRGDEAVRLCSSLLDQGKIVAIKGIGG
                     FHIACTPFSDETVALLRDRKRRAHKPFALMVSSLEIAEELTYLPAVAKRLMESPASPV
                     VLCCVRPKPRSSDVAAVSPLVAPGQRTLGLMLPYTPVHHLLLEGKRALIMTSANFSDA
                     PIVSDNAAALASLSSIADFFLFSNRDIEMPIDDSVAMPFGRSFFLLRRARGYTPLPMN
                     FRRGDSCVIFGAGAEMKGSFCFSRDDVLLPGQYLGDMKQRDTVKYYNRALEHFVKLYE
                     LKPEKVAFDMHPQYLSTQIAKKVAENYSAETVPVQHHHAHFAACLFENNVEETSAGIL
                     FDGTGYGEDGTIWGGEIFIGNRSRYERIGHFYPSRLPGGDAAVMEPWRYALGLLHDVY
                     GKEEASVMGERLWSAHSQKVRLVLDILPSSPETTSCGRFFDAAAALLGCGEVVTFDGQ
                     AAMSLEAMARGTLIAPFLVTRQDGRFIIDWRPVIRWILDSMETMPAPDIAGGIHGGLA
                     RAVADICGTLRKERGIRYAALSGGVWQNRRLAAITSIFLRRRGVEPLLHKLLPPNDEC
                     VSAGQTVVAAEFFNHEER"
     gene            complement(2001..3014)
                     /gene="glpX"
                     /locus_tag="PK849_05885"
     CDS             complement(2001..3014)
                     /gene="glpX"
                     /locus_tag="PK849_05885"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HPE65692.1"
                     /translation="MCAADIFAADRNLALELVRATEAAAMAAGRWMGRGDKNAVDGAA
                     VNALRYMMNTVPMSGVVVIGEGEKDEAPMLFNGEQLGASDEPQMDIAVDPVDGTRLTA
                     LGLAGAISVVALSERGTMFNPKNIFYMNKLVTGPDAADVIDINLSPEENIVRVAQAKK
                     KAVDDVTVVVLDRPRNDEIKDHIRKAGARIKLIPDGDIAGALLTCKDQGGADLLMGIG
                     GSPEAVITACAIKCLDGNMQCKLWARNESDAEESRKRGLDLDQVLTIHDLVQGDNIFF
                     AATGVTDGAFLQGVRYEGRQIRTSSMVMRSKSGTIRFVDSIHVPKKLSELSKVSGIDY
                     STV"
     gene            complement(3170..4468)
                     /gene="purB"
                     /locus_tag="PK849_05890"
     CDS             complement(3170..4468)
                     /gene="purB"
                     /locus_tag="PK849_05890"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPE65693.1"
                     /translation="MIDRYTTKEMNAIWSEENKFRTWLEVELAVCKVWNERGVIPSEA
                     YDEICAKADFRIERINEIEEQVQHDVIAFVSAVAETVGKNGRYIHLGLTSSDVIDTAS
                     SLSLKRSLTVVDEKVVQLISAVNKRAEEFRYTPCAGRTHGIHGEPTTFGLKLMNWQYQ
                     LQRDRQRIALAVEQINYGKISGAVGTYAHCDPGLEKRVCDLLGLRPALVSTQILQRDR
                     HANILNTLALLGSALDRFATEIRHLQRTEVMEVLEPFGARQKGSSAMPHKRNPILCER
                     ISGMSRLLRGYALTGMENINLWHERDISHSSAERVVWPDAFHTICYMLSKMKTVVENL
                     RVDTEKMKENLELTRGLVYSQRILLELVERFGMLREEAYAIVQENAMKCWQEKIPFCD
                     LLLRDERVSSLVSETQLRSYFAPEYYFRWVDEVFSRFSDE"
     gene            complement(4461..5024)
                     /locus_tag="PK849_05895"
     CDS             complement(4461..5024)
                     /locus_tag="PK849_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPE65694.1"
                     /translation="MSVSTTDKPSRNTAYFYPVKLIVGVLYPDEALWTWTKNALAALW
                     GSPEDESAPYLFSSLTDYYADIAPVLFRRFLSFQTLRDAGELQEWKISACETEKRSGT
                     PRKVNVDPGYVNGARLVLASTKDHAHRIYIGGGIHAEVTLRYRRKRWTPFDYTFPDFA
                     DGRYNDFLSLVRKRWLDEMTARRLRND"
     gene            complement(4993..5574)
                     /locus_tag="PK849_05900"
     CDS             complement(4993..5574)
                     /locus_tag="PK849_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65695.1"
                     /translation="MEPLTPVLLVTDRGVELVPAGKIPQPSPKQECTAPDEGTTDSGL
                     LCWWTIPLPLAFLTGKKPVLNPAADKLLDGFTLPSFARKKKHPTEFLIELGSKSTKKR
                     NTISRHFLFTEVKDSFFLIQEVTEDLQSAEQMAWLASIGQAFLSDLKCKGIPVRQIRS
                     PEYTAEEENGRRLACIWEEKLLGYVCIDNGQTE"
BASE COUNT         1253 a         1546 c         1515 g         1477 t
ORIGIN      
        1 tatcgctacc cttttatcaa ctgtaccgac tgtggtcccc gttacactat tatacgcgaa
       61 cttccctatg atcgtccgaa gaccacaatg gcttcgtttg ttatgtgccc caagtgcagc
      121 agtgagtatg cggatttgga gcacaggcgt tttcatgccg agccgaatgc gtgccccctc
      181 tgcggtccgg aggtctggct tacggattcg aatggaacgg agctgacaag gggggacgaa
      241 gctgtccgac tttgttcctc gctgcttgac cagggcaaaa tcgttgcaat aaaaggtata
      301 ggcggttttc atattgcgtg tacccctttc agtgacgaga cagttgcctt gttgcgagac
      361 cggaagcgaa gagcgcataa accgtttgcg ctgatggttt cttctcttga aattgcagag
      421 gagcttacct atctgcccgc tgtcgcaaag agactcatgg aatctcccgc aagtcctgtg
      481 gtgctctgct gcgttcgccc gaagccgcgt tcctccgatg tcgccgctgt atctcctctt
      541 gttgctcccg ggcagcggac tcttggatta atgctcccgt acacgccggt gcatcatctg
      601 cttctcgaag gaaagagggc gcttatcatg accagcgcga acttttcgga cgccccgatc
      661 gtctccgaca atgctgcggc tcttgcctcg ctctcctcaa tcgcggactt tttccttttt
      721 tcgaatcgcg atatcgagat gcccatcgac gattccgttg cgatgccgtt tggacgttca
      781 tttttcctcc tgcggcgcgc cagagggtac actccgcttc cgatgaattt ccgccgtgga
      841 gacagctgcg ttatcttcgg tgcgggcgcc gagatgaaag ggagcttctg tttctcccgg
      901 gatgacgtgc tgctccccgg gcagtatctt ggcgacatga aacagcgcga caccgtgaag
      961 tactacaaca gggcgctgga gcacttcgtg aagctctacg agctgaaacc agagaaggtc
     1021 gctttcgata tgcaccccca gtatctttcc acgcagatcg cgaaaaaggt tgccgaaaat
     1081 tactccgccg aaaccgtacc ggttcagcat catcatgcgc acttcgcggc gtgtcttttt
     1141 gaaaacaacg tcgaagagac ttctgccgga atactctttg atggtactgg atacggcgag
     1201 gacggcacta tctggggcgg ggagattttt atcggaaaca gatcgcgtta tgagcggata
     1261 gggcattttt acccctcgcg gcttcccgga ggagacgcgg ctgtaatgga accatggcgc
     1321 tatgctcttg gacttttgca cgacgtatac gggaaggagg aggcctctgt catgggagaa
     1381 aggctgtgga gcgctcattc acagaaagta cgactggttc tcgacattct cccctcctcc
     1441 ccggaaacga catcctgcgg acgcttcttt gatgctgccg cagctttgct ggggtgcgga
     1501 gaggtcgtca cttttgacgg acaggcggcg atgagtctgg aggcaatggc cagaggaacg
     1561 ctgatagctc cttttcttgt tacgcgacaa gacggccgtt tcattatcga ttggaggcct
     1621 gtgatccgtt ggattctcga ttcgatggag actatgcctg ctccggatat cgcgggaggc
     1681 atccatggcg gtcttgcccg ggcagtcgcg gatatttgcg gtacgctccg taaagagcga
     1741 gggattcgtt atgctgccct ttcgggcgga gtatggcaga atcggcgact cgcggcgata
     1801 acatccattt tcttacggcg gcgaggagtg gagcctcttc tccacaaact cttgcccccg
     1861 aacgacgagt gcgtctctgc ggggcaaact gtggttgcgg cggaattttt caatcatgag
     1921 gagcgataaa aaacgcctgc gggatatgca gagtgtcccg caggcaaaag agggtttccg
     1981 cttctcttaa gggctgtgtg tcaaaccgta ctgtagtcta ttccgcttac cttggagagc
     2041 tccgagagtt ttttcgggac atggatgctg tccacaaaac ggatagtgcc gctttttgag
     2101 cgcatgacca ttgagctggt gcgaatttgg cgtccctcat aacggacgcc ctgaaggaac
     2161 gctccgtcgg tgaccccggt tgccgcaaag aatatgttgt cgccctgcac aaggtcatgt
     2221 attgtcaaca cctgatcgag gtcgagtcct ctcttcctgc tttcctcggc gtcgctttcg
     2281 ttgcgggccc acagtttaca ctgcatgttt ccgtccaggc acttgattgc acatgcggtg
     2341 atgactgctt cgggcgagcc gcctattccc ataaggagat cggcgccgcc ctgatcttta
     2401 cacgtaagaa gcgctcccgc gatatcgccg tcggggatga gtttgatcct cgcgccggct
     2461 tttcggatgt ggtccttgat ctcatcattg cgtggcctgt cgagaacgac aactgtgacg
     2521 tcgtccacgg cttttttttt cgcttgcgcc actcggacga tattctcctc cggtgaaaga
     2581 ttgatatcga tgacgtctgc tgcgtccggg cctgtaacca gtttgttcat atagaaaata
     2641 ttcttcgggt tgaacatcgt tcctctttcc gagagcgcga ccacgctgat cgctccggca
     2701 agtccgagag ctgtcaagcg agttccgtcg accggatcca cagcgatgtc catttgcggt
     2761 tcgtcggaag cgccaagctg ttctccgttg aagagcatgg gggcttcgtc tttctcgcct
     2821 tctccaatga cgacaacccc ggacatcgga acagtgttca tcatgtatcg aagcgcattg
     2881 acggccgcgc cgtcgacggc gttcttgtcg ccgcgcccca tccatcttcc cgcagccata
     2941 gccgcagctt cggttgcccg taccaattcg agagcgaggt tcctgtcagc agcaaaaata
     3001 tctgcagcac acattgagat atcctccttg aaattagtat tttacaaggc caatcgttca
     3061 ctttggtaca actgcaaaaa ggacagtatc cgagtacctt gtcgcttgct agtaattccc
     3121 ccctaagaaa ccccttagcg ttctctacct tattttgttg cggatatttc tactcatcgg
     3181 aaaatcggct gaatacttcg tcaacccatc ggaaatagta ttccggtgca aagtacgaac
     3241 gcaactgtgt ttcggaaacc aaagaagaaa ccctctcgtc acgaagcaga agatcgcaaa
     3301 acggaatctt ctcttgccag catttcatcg cattctcctg aactatggcg tacgcctcct
     3361 cgcggagcat cccaaatcgc tcgacgagtt ccaggaggat acgttgactg tacaccagtc
     3421 cgcgcgttaa ttcaaggttc tctttcattt tttcagtatc gactcgtaaa ttttcaacta
     3481 cagtcttcat tttcgaaagc atatagcaaa ttgtgtgaaa agcgtcgggc cagacaactc
     3541 tttctgcgga cgagtgactg atatcccgtt cgtgccagag attgatgttt tccattcctg
     3601 tgagcgcgta gcctcgaagc agcctgctca taccggaaat acgttcacag agaatcgggt
     3661 tccgcttatg cggcatggcg ctcgaccctt tctgccgtgc gccgaaaggt tccaggactt
     3721 ccataacctc ggttctctga agatgacgga tctccgttgc aaaacggtcg agggcgcttc
     3781 caagaagagc gagcgtgttc aaaatgttcg catgccggtc gcgctgcaga atttgtgtgg
     3841 aaacgagggc aggccgaagc cccagtaagt cgcaaacccg tttttcaagg ccgggatcgc
     3901 agtgagcgta cgtgcccact gccccggaga tttttccgta atttatctgc tcaaccgcaa
     3961 gagcaattcg ctggcggtct ctttgaagct gatattgcca gttcatcagt ttcaatccga
     4021 aagtagtcgg ctctccgtga atcccatgtg ttctgccggc gcacggcgtg tatcggaact
     4081 cttccgcgcg tttgtttaca gccgaaatca gctggactac tttttcgtcc actacagtca
     4141 gagaacgttt gagtgaaaga gaagaagcag tgtcgatgac atcgctgctc gtcagtccta
     4201 gatgaatata gcggccgttc ttcccgacgg tctccgcaac ggctgagacg aaagcaatga
     4261 cgtcgtgttg tacctgttct tcgatctcgt tgatccgttc aatacgaaaa tccgctttcg
     4321 cgcatatttc gtcgtacgct tcagacggga taacgccgcg ttcgttccac actttgcata
     4381 cggcgagttc aacttctagc catgtcctga acttgttctc ctcgctccaa atagcattca
     4441 tttctttggt tgtatatctg tcaatcattc cgcagcctcc tcgcagtcat ttcatcgagc
     4501 cagcgtttcc gtacaagcga gaggaaatcg ttgtaacgtc cgtccgcaaa atcggggaag
     4561 gtgtaatcga agggcgtcca tcgctttcgc ctgtaccgca acgtcacctc cgcatggatc
     4621 cccccgccga tatagattct gtgcgcatgg tctttcgtag aggcaagaac gagtctcgct
     4681 ccgttcacat acccgggatc gacgtttact tttctcggcg tcccgctcct cttctccgtc
     4741 tcacatgcgg aaattttcca ttcctgcagc tctcctgcat cccggagagt ttggaacgag
     4801 agaaaacgtc gaaacaaaac cggagctatg tcggcgtaat aatcagtgag cgaagaaaaa
     4861 aggtacggag cgctttcatc ttcgggagat ccccacaggg ctgccaacgc attctttgtc
     4921 cacgtccaaa gagcttcgtc ggggtagaga accccgacga tcaacttcac aggatagaag
     4981 tacgcggtgt tcctactcgg tttgtccgtt gtcgatacag acatatccaa gaagtttttc
     5041 ctcccatatg cacgccaggc gccttccgtt ctcttcttcg gctgtatatt cgggcgaacg
     5101 aatctgccgg accggtattc ctttgcactt gagatcggaa agaaatgctt ggccgatgga
     5161 agcaagccat gccatttgtt ccgcactctg cagatcttcc gtaacctcct ggatcaggaa
     5221 aaaagagtcc ttcacctcag tgaacagaaa atgccttgag atagtatttc gctttttcgt
     5281 tgatttgctg ccgagttcga tgaggaactc agtggggtgc tttttttttc gggcgaaaga
     5341 aggcagtgta aacccgtcga ggagtttgtc ggcagcaggg tttaaaaccg gttttttccc
     5401 cgttaagaaa gcgagaggaa gcggtattgt ccaccagcat aggaggcctg agtccgttgt
     5461 tccctcgtcc ggagctgtgc attcttgttt gggggaaggt tgcggaattt ttcccgcggg
     5521 aacgagctca acacccctgt cagtaaccag taaaaccgga gttaaaggtt ccattagacg
     5581 ctgttcagcg aggcaggcga aaccgtttga tgaaagtaac gcgattgcat cgccgacttt
     5641 tgtatcggcg tttcctcttt caagaagaga gacaatctga tcctttgagt agagtgaaaa
     5701 accttgagat gtcgcaagcg cgcaatgctc aactccgtgc agatagaggg agcgaagaag
     5761 cgacgacaga ggagattcga gcgtagtgtt g
//
LOCUS       DAOPKU010000146         5761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_15020_length_5761_cov_5.470032, whole genome shotgun sequence.
ACCESSION   DAOPKU010000146 DAOPKU010000000
VERSION     DAOPKU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15020_length_5761_cov_5.470032"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PK849_05905"
     CDS             complement(<1..972)
                     /locus_tag="PK849_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65696.1"
                     /translation="MMKLNRLGSRILVVVLALLIVSVGGIGFYAMKIAGDNTRRMVND
                     YEISVANGLAHELDAVFNRFDGILQALSSLVSTRFTPAMLNADVADVVVRQFASQNGK
                     FVADLGAQSVEARSLFMIFNPEIFGVKKVFSVGFQRKDQDSLYMFMDSEQFSAAKLID
                     RKEPSTSWFWTPLDTGRPYWGDLARSETGEELVTYTMPVVIGDKIAAVAGMSFDFSFV
                     RTMLASVKIYDTGYPFLMNRDLRFLYHPSLPFDGPDLRKVSDGSMASFADAMLKEPSG
                     RFSYFYEGDEKTLAYKSLANGYIVAAAATTAESLAAVGAMQRAVYIGI"
     gene            complement(1064..1480)
                     /locus_tag="PK849_05910"
     CDS             complement(1064..1480)
                     /locus_tag="PK849_05910"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPE65697.1"
                     /translation="MHSSDTTKKQPKKIAAPGQESRRFVRVECVLRAEFRLHNHDAWL
                     PAEVLDLSVAGMKLRFNPFQRGRTLLPEVFEWADSRFRFPLKREFFHLDGHFLKIYQR
                     SAGLFTAGVEFDEPSPEDQFKLVELYAEFRRRRGAI"
     gene            1670..3343
                     /locus_tag="PK849_05915"
     CDS             1670..3343
                     /locus_tag="PK849_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPE65698.1"
                     /translation="MKDLFERTEFLLNGGVLTLLSDSLAAHAARGVILRPEGEDLTVL
                     EAFGAGVESLFLKGRARDIQNAFPSPFLREAYDVAAAPLPGEWPWGIRLRGRDWVFFI
                     LLSEEPPKELIDQMPSFAGLLSLWQKHQKTAGTEERLSKLAYMVLATKSTLASVFEPM
                     GLDYFGAFLCDVLTESLFPSRLSVVLDKGGRLSHLDGEDIQMPPRKGIFARDILSPVP
                     VRLEESMRDSVGEAVFHDLAGEWSAILPIIGGDVRLFCLLRWETLPGEEALNFMELLG
                     NVASKALSMSAMREEKERNMQELSRRAFLLNALHEAALKFLEQTSKEELLLQILDIFS
                     EMTQAPRAVLVAWQPHAGGYVVFASKEDGILQRTGRPAGFPIGILEGDRPSSFSMEEG
                     KRLFAFMEVPFLASLDAMEGMKTIFPLWDGDRLIAFIAVSEPLPGTVTMDLETLEILA
                     RTASAAMLSREWESARLASGKYLNVAALVHWESERIREEIVAEGGTPMLVKGPFGVSV
                     SAEQERLCRLVVRMEDATVCVAEESSEALAAFFPSAAGWTVQKFHAGQK"
     gene            complement(3350..3994)
                     /locus_tag="PK849_05920"
     CDS             complement(3350..3994)
                     /locus_tag="PK849_05920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPE65699.1"
                     /translation="MRAAFLLLLVFFAIPSFAEASGYLRNGNGHTVALTFDDGPRPGY
                     VEPILAILREKDVRATFFLVGRYVLEHPELVRAIDAEGHTVANHTFYHNNLTSLPPEN
                     VYREWRLCNEAVERVTGKKPRFARPPGGRYNTFVAERANEEGLRIVLWTNNPGDYAAS
                     LDGPSLARKVLSRAKEGDIVLLHVGVAPTIDALPEIIDGYRRKGFSFVTVDEMR"
     gene            4388..5134
                     /locus_tag="PK849_05925"
     CDS             4388..5134
                     /locus_tag="PK849_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE65700.1"
                     /translation="MISYFDMSELKDGVTRQVYLALRRDILNWRLLPGRRLSEKDIAG
                     RMNISKTPVREAFIKLQEEGLLEIRPQRGTFVSLMDMEQIEEVRFTRLCVESTVLSLF
                     IESPDENALASMRSILDLQEGAVPDRDIDAFVEYDDAFHREIFLACGKARTWSFIDHF
                     SAQYKRLRYLSLQNYLDFGIVLAEHRAILSACEKRAGEDARRCLSKHLNKILGESEHL
                     REKFPEYLLKDGGSWHPQGESLQGLCVECP"
     gene            5201..>5761
                     /gene="dctP"
                     /locus_tag="PK849_05930"
     CDS             5201..>5761
                     /gene="dctP"
                     /locus_tag="PK849_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE65701.1"
                     /translation="MKRTVRFAVSLMMVFALALCAQAAPADLVEGDGNTYELMISTQL
                     ADASPFCQGFYALAERVKERSGGKMVVKVFPSAQLGSDEDVIEQAIQGVNVAVVTDAG
                     RMANYVKEIGIVGMAYFTNSYDELLKVQQTDFWKGNVEKLASENGIRVLSFNWFDGAR
                     HFLTNKPVKTPEDLKGVRIRTPGAPSW"
BASE COUNT         1144 a         1744 c         1736 g         1137 t
ORIGIN      
        1 gatgcctatg tagacggcgc gctgcatcgc gccgaccgcg gcgagcgact cggcggtcgt
       61 cgcggctgcc gcgacgatgt acccgttggc gaggctcttg tacgccagcg ttttttcatc
      121 tccttcatag aagtacgaga agcgtccgct cggctctttc agcatggcgt cggcaaagga
      181 ggccatggag ccgtccgata cctttcgcaa atcggggccg tcgaacggga gcgaggggtg
      241 gtagagaaaa cggagatcgc ggttcatcag gaaggggtag cccgtgtcgt aaatttttac
      301 cgacgccagc atcgtgcgga caaacgaaaa atcgaaggac atgccggcga cggcggcgat
      361 tttatccccg atgacgaccg gcatggtgta ggtgacgagc tcctcccccg tttcggagcg
      421 ggcgagatct ccccaatagg gacgccccgt gtcgagcggc gtccagaacc acgaggtgga
      481 gggctccttc cggtcgatga gtttggcggc ggaaaactgc tcgctgtcca tgaacatgta
      541 gagcgagtcc tggtcttttc tttggaaccc cacgctgaag accttcttga cgccgaagat
      601 ctcggggttg aatatcatga agaggctgcg cgcttcgacg gactgcgctc ccagatctgc
      661 gacgaacttg ccgttctggc ttgcgaactg ccggaccacg acgtcggcga cgtcggcgtt
      721 gagcatcgcc ggagtgaacc gggtgctcac aagcgaggaa agcgcttgca gtatgccgtc
      781 gaaacggttg aagaccgcgt ccagttcgtg cgcaagtccg ttggctacgg agatttcata
      841 gtcgttcacc atccggcgcg tgttgtcgcc cgcaatcttc atggcgtaaa atcctatgcc
      901 gccgactgat acgatcagca gcgcgagcac gacaacgaga atgcgggaac ccagccgatt
      961 cagtttcatc acatcatctc ctccaaaaga atagagaata cgctactctt tttattgcca
     1021 taaagcaagc gcccctcgcg cagcgctaaa ggaagaaggg gaatcagatc gcgcctctcc
     1081 gtctccggaa ctccgcatag agctcgacga gcttgaactg gtcctccggc gaaggttcgt
     1141 cgaactccac tcctgccgtg aagaggcctg cgctccgctg gtagatcttc agaaaatgcc
     1201 cgtcgagatg aaaaaactct cgtttcaggg gaaagcggaa acggctgtcg gcccactcaa
     1261 atacttcggg gagcagcgtt ctccctctct ggaaggggtt gaacctgagc ttcatgccgg
     1321 cgacgctcag gtcgagcact tcggcaggca gccaggcgtc atgattgtgc agccgaaact
     1381 ccgcgcggag gacgcactcc acccggacaa aacggcggct ctcctgtccg ggcgccgcta
     1441 tttttttggg ctgttttttc gtcgtatcgg aagaatgcat aattcaccct gtgttttgtc
     1501 gaagaacgcc cccggcgtcc ccggattttt ttgtggcgtc ttttgcaact ttaagatacc
     1561 atagaggagt cttcctgact acagaaggag cgtcgccccg aacttgggac aggggaaccc
     1621 gggcacggga tcgtgtatat ccgcaccaat gaagggatgt tgatggttca tgaaggatct
     1681 ttttgaacgg acggaatttc ttttgaacgg aggcgtcctc acgctgcttt ccgacagcct
     1741 cgccgcgcat gcggcgcgag gggtgatcct ccggccggag ggagaggacc tgaccgttct
     1801 cgaagccttc ggcgcagggg tggagagtct tttccttaaa ggaagagccc gtgatattca
     1861 aaacgcgttt ccgtccccct tccttcgcga ggcgtacgac gtggcggcgg cccccctccc
     1921 gggggagtgg ccgtggggga ttcgtctccg cgggagagac tgggtctttt tcatcctcct
     1981 ctcggaagaa cccccgaaag agctgatcga ccagatgcct tcattcgccg gactgctcag
     2041 cctctggcag aaacaccaga agaccgccgg aacggaggag cgtctctcca agctggcgta
     2101 catggttctg gccaccaaga gcacgctcgc gtcggtcttc gaaccgatgg ggctggacta
     2161 cttcggcgcg tttctctgcg acgtgcttac ggagagcctg tttccttcgc gtctctccgt
     2221 cgttctggat aaaggcggtc gtctctcgca cctcgacggc gaggatatac agatgcctcc
     2281 ccgcaaaggg atcttcgcgc gcgacattct ctctcccgtt ccggtgcggc tggaggagtc
     2341 aatgcgcgac tcggtcggag aggcggtctt ccacgacctc gcgggcgagt ggtccgccat
     2401 tctcccgatc atcggcgggg acgtcaggct cttctgtctt ctccgctggg agacgctccc
     2461 cggcgaggaa gctctcaact tcatggagct gctggggaat gtcgcatcga aggcgctctc
     2521 catgtccgcg atgcgggagg agaaggagcg gaacatgcag gagctttcac ggagagcctt
     2581 cctgctgaac gcgctccacg aggcggccct gaaattcctt gaacagacct cgaaggaaga
     2641 gctgctgctc cagatactcg acatcttctc cgagatgacc caggctccgc gcgcggtgct
     2701 tgtggcctgg cagccgcacg cgggcggcta cgtcgtcttc gcctcgaagg aggacggcat
     2761 cctgcagcgt accgggcgtc cggcgggctt ccccatcggc attctcgaag gcgaccgccc
     2821 gtcctccttc tcaatggagg aggggaagcg gcttttcgcc tttatggagg tccccttcct
     2881 cgcgtcgctc gacgcaatgg aaggaatgaa gacgatcttc cccctctggg acggcgaccg
     2941 ccttatcgcc tttatcgcgg tatcggagcc gcttccgggt actgtcacca tggatctgga
     3001 aacgctggag attctcgccc gcaccgcgtc ggccgcgatg ctgagccgcg agtgggagtc
     3061 cgcccggctc gcctccggca agtatctgaa cgtcgccgcg ctggtgcact gggagagcga
     3121 gcgcatccgc gaggagatcg tcgccgaagg agggacgccg atgctcgtca aagggccctt
     3181 cggcgtttca gtatccgccg agcaggagcg gctctgccgc ctcgtcgtcc ggatggagga
     3241 cgccacggtg tgcgtcgcgg aggagtcttc cgaagcgctg gccgcctttt tcccctccgc
     3301 cgcggggtgg acggtccaga agttccacgc cggtcagaaa taggcgaagt taccgcatct
     3361 cgtcgacggt gacgaaggag aaccctttcc tacggtagcc gtcgatgatt tccggcagcg
     3421 cgtcgatcgt gggcgcgacg ccgacatgca gcagcacgat gtcgccctct ttcgccctgg
     3481 agagcacctt ccgggcgaga gaggggccgt cgagcgacgc ggcgtagtcc cccggattgt
     3541 tggtccacag tacgatgcgc agcccctctt cattcgcccg ctccgcaacg aaagtgttgt
     3601 agcgcccccc gggcggccgg gcgaaccgcg gttttttgcc ggtaacccgc tccacggcct
     3661 cgttgcagag ccgccactcg cggtagacgt tctccggcgg cagcgacgtc aggttgttgt
     3721 ggtagaacgt atggttcgcc accgtatgcc cctccgcgtc gatcgcccgc accagctccg
     3781 gatgctccag aacgtaccgt ccgaccagga agaacgtcgc cctgacgtcc ttctcccgca
     3841 gaatagccag tatcggctcg acgtaccccg gacgggggcc gtcgtcgaag gtcagcgcca
     3901 ccgtatgccc gttcccgttt ctcagatagc ccgaggcttc cgcaaacgag gggattgcga
     3961 agaagacgag cagaagcaaa aatgccgctc tcatttgtat tctcttgttc atagtcacct
     4021 ttccattctt ctgaatgcgt caaagagcac atgatagcac tctccgcatc gtcgcgccac
     4081 acaattttgc cgaatccgca ggcagaaaga ggcttcaccc gcgtccgtat caaacgaggg
     4141 aaaaccaatt tcacaaacct cgtcccgaaa ccacttgcga agtcaatgaa aaactgcaaa
     4201 ttccgggtgc gctgaaaccc gattcacctc tgaatgaaaa gtgaagaaaa tttcgttcgc
     4261 ccccttgaca aaggccaaag cgatggctaa catactaaca tatcagtttt ccattttctc
     4321 tagcgtgaat tatgcgacac gcttcggctc cccgccgggg cgatcttcag ggaaagaggg
     4381 ccttgccgtg atctcgtatt tcgacatgag cgaactgaag gacggcgtca cgcggcaggt
     4441 ctacctggcg ctccgcaggg acatactgaa ttggcgtctt ctccccggaa ggcggctcag
     4501 cgagaaggac atcgccgggc ggatgaacat cagcaagacc cccgtccgcg aggcgttcat
     4561 caagctgcag gaggagggac tcctcgaaat ccgcccccag cgcggcacct tcgtctcgct
     4621 catggacatg gagcagatcg aggaagtccg cttcacccgc ctctgcgtcg agagcaccgt
     4681 gctctcgctc ttcatcgaat cccccgacga aaacgctctt gcctccatgc gctccatcct
     4741 cgaccttcag gagggcgccg ttccggaccg ggacatcgac gcgttcgtgg agtacgacga
     4801 cgcgttccac cgcgagatct tcctcgcgtg cggcaaagcc cgcacgtgga gcttcatcga
     4861 ccacttcagc gcgcagtaca aacggttgcg atacctcagc cttcagaact acctggactt
     4921 cggcatcgtc ctggcggagc accgggcgat tctctcggcc tgcgaaaagc gggcggggga
     4981 ggacgcgcgg cgctgcctct cgaagcatct gaacaagatt ctgggcgagt cggagcacct
     5041 cagggagaag tttccggagt accttctgaa ggacggaggt agctggcatc ctcaaggaga
     5101 gtccctgcag ggactctgcg tcgaatgtcc gtaatttcgg cgagttgttg gagtatcggc
     5161 ctatcggcca gtaatataca aaaacagggg aggaattgaa atgaaaagaa cagtgaggtt
     5221 tgcggtatcg ttgatgatgg tgttcgcgct cgctctctgc gcgcaggcgg ctccggcgga
     5281 tctcgtcgaa ggcgacggca atacgtacga actgatgatc tcgacccagc tggccgacgc
     5341 cagccccttc tgccaggggt tctacgcgct cgcagagcgt gtgaaggagc gcagcggcgg
     5401 aaagatggtc gtcaaggtct tcccgagcgc ccagctcggc agcgacgagg acgtcatcga
     5461 gcaggccatt cagggagtca acgtggccgt ggtcaccgac gccggccgca tggcgaacta
     5521 cgtcaaggag atcggcatcg tcggcatggc ctacttcacc aacagctacg acgaacttct
     5581 gaaggtccag cagacggact tctggaaggg gaacgtcgag aagcttgctt ctgagaacgg
     5641 aattcgcgtg ctctccttca actggttcga cggcgcgcgg cacttcctca cgaacaagcc
     5701 cgtgaagacc cccgaggatc tgaagggcgt ccgtatccgc accccgggcg cgccctcctg
     5761 g
//
LOCUS       DAOPKU010000147         5650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_15389_length_5650_cov_4.306524, whole genome shotgun sequence.
ACCESSION   DAOPKU010000147 DAOPKU010000000
VERSION     DAOPKU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15389_length_5650_cov_4.306524"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2203
                     /locus_tag="PK849_05935"
     CDS             <1..>2203
                     /locus_tag="PK849_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65702.1"
                     /translation="AEARWSLDGEGSYASGESAKDVVVGKHTVSFSDIADWDKPADGT
                     VEVAKDAEAAFKGAYAQHKGSLQVVMTDGPAGARWSLNGKGSYKDGETVNDLVVGEYS
                     VSFLDLADWDEPAEQTVKVTKNAVAKLEVELVQHKGSVQVVLFGPEEARWSVDGKGSY
                     ASGETVKDLIVGTYRLSFSQVDGWDAPREMRITVAKDTLAKTEPGYTRHLGSVTVTID
                     GPAEARWSLDGQGSYESGQSVDKVVVGEHTVSFSDIADWDTPADQTMSVTKDAASTVT
                     GNYKQHKGSVKITIKGPEEARWSLNGEGSYASDAVVEDLVVGEYAVTFSKIAGWDRPR
                     EQKLVVGKDATATFEGTYTQHVGAVAVYIEGPSEALWKISGIEESHAGGEAAKDLPVG
                     EYTVSFSDADGWEKPEDQPVTVFRNVTAMATGVYTRHVGSVSTVIEGPESALWKLNGE
                     GNYSSGEVVQGLPVGEYVVSFGSVEGWEKPEDQPVVVTKDGMASVSGVYSQHTGSVSA
                     VIDGPESALWSVNGEGNYASGEVVQKLPVGDYVVSFGGVEGWEKPEDQKVVVTKDELT
                     SISAVYTRQKGSVSAVIEGPETALWSLNGEGSYASGEVLQGLPAGEYIISFSEVEGWK
                     TPANIAVSVLKDSLASVTGSYAQPAVEEPAVEESATTEKPETAEEPKAEEPAVEEVKE
                     EIVSEEPKAEEAVVEEVKEEAASEEPAAVEEPKAEEPAEKAEEAATEAVAE"
     assembly_gap    2204..2303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2304..2946
                     /locus_tag="PK849_05940"
     CDS             <2304..2946
                     /locus_tag="PK849_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65703.1"
                     /translation="VAEEPKPEEPAVEEVKEEAASAEPVAAEEPKAEEPAEKAEEAAT
                     EAVAEQSAVAEEAAREVAAEVVEAVKETAEEVQEVVTGEPVVTEEPKPEEPVVEEVKE
                     EVASEEPVAVEEPKAEEPAVEEVKEEIVSEEPKSEEAVVEEVKAEPVSVEPVAVEEPK
                     PEEPAAEAEQEAAVEEAAKEAAEVPAPESPAPETVQQDKQTEKPAGATAAPCD"
     gene            3156..5015
                     /locus_tag="PK849_05945"
     CDS             3156..5015
                     /locus_tag="PK849_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPE65704.1"
                     /translation="MYGYWGKLLRVDLTHRTHRIEDIPEEVFDLLLGGSALGAKILLE
                     ETPANVDPFSPENKLIFSVGPFQGVNFPGNGKWSVITKGPLTGTYLESAGTGHWAPYF
                     KKCGYDAVVFEGKASSPVYVVLRDDSVEFRDASFIWGKDTVETGELIKEDLGDKRINA
                     LNIGPAGEIGNPIACITCDGHSFAGRGGAGAVMGSKNLKAVAAWGTKEVPVFDKEKAS
                     EYSKEIFKLLHEQGEGFRQHGTPVVMVPLEEIGDVPIKYWRGDENKYGAYLLGAPRYT
                     EYLNVKPLPCINCPVGCHRHIHFEFPDGTVLDGNGPEYETIGMMGTNLLMEDLSAVAV
                     ANDIANRMGIDTVSCGAWIGFLMECWEYGWITPKETDGITIKWGDPEVLVELTQQIAS
                     MSGVGEWFREGVRGAAKKIGKDAEKITVEVKNLDYPAHDPRAVYGLGVNYAVGTRGAC
                     HERGNPQASALGLFFPELGQDAPYDRQDPKFAAKTAILHQDVSALHNCMTLCKFMMGG
                     AGLTLTEMNTAFKHLTGKDVPVPEFVKYGERAWQLQRLINVRDGMNRKDDTLPLKMTI
                     PAMVGPRAGKAPTPHDEILDDYYAQRGWDKNGVPTKERLEALGLGAYVKYLGR"
     gene            5041..>5650
                     /locus_tag="PK849_05950"
     CDS             5041..>5650
                     /locus_tag="PK849_05950"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HPE65705.1"
                     /translation="MMQAKVGWSTAGSPLAAGKEAAQAAKVDGAQIALLFGSVDYNAE
                     ELLKGVRETLGDVPVIGCTSYTGVLTPGGFITSPDGYCALMALSDEDMAVSVGAAEKK
                     ECAVAAGEEAALAALEAAGMDMAPEFFYMVAPPGEEEFYLQGIQSVIGRVPFFGGSAA
                     DNDLSGKWLVYGNGVVKNGVAVAFFWNKAYGNKYTGAYRGTGK"
BASE COUNT         1351 a         1372 c         1819 g         1008 t
ORIGIN      
        1 ccgccgaagc tcgttggagc ttggacggcg aggggagcta cgcaagcgga gagagtgcaa
       61 aagacgtcgt tgtcgggaag cacacagtct ccttctccga tattgccgac tgggacaagc
      121 ctgccgacgg tacagtggaa gtcgccaaag acgccgaggc ggcctttaag ggagcatacg
      181 cgcagcacaa gggctcgttg caggtcgtta tgaccgacgg ccctgctgga gcgcggtgga
      241 gcctgaacgg taaaggttcg tacaaagatg gagagacagt aaatgatctt gttgtcggag
      301 agtattcagt gtctttcttg gatcttgccg attgggacga accggcggaa cagactgtca
      361 aagtcacgaa gaacgccgtt gcaaagctcg aagtcgaact cgttcagcac aagggatccg
      421 tgcaggttgt actcttcggt cctgaagaag cgcggtggag cgtggacggc aaagggagtt
      481 acgcgagcgg cgaaactgtc aaagatctta ttgtcggaac gtataggctt tccttctctc
      541 aggttgacgg ttgggatgct ccccgtgaga tgcggatcac cgtggcgaag gatacgcttg
      601 cgaaaactga gccagggtat acgcggcatc tcggctccgt gacggtgacg atcgacggtc
      661 cggcggaagc gcgctggagc ttggacggcc agggaagcta cgagagcgga cagtctgtcg
      721 acaaagttgt ggtcggcgag catacggttt cgttctctga tattgccgac tgggatacgc
      781 ctgccgatca gacgatgtcc gtgacgaaag acgctgcgtc gaccgtcaca ggaaactaca
      841 aacagcataa aggctcggtc aagatcacga tcaaagggcc tgaagaagcg cgctggagtt
      901 tgaacggtga agggagctat gcgagcgacg ctgtcgttga ggatctcgtc gtcggagaat
      961 acgctgtcac gttttccaag atcgccggct gggacagacc tcgcgaacag aagctcgtcg
     1021 tcggcaagga tgcgaccgca acgttcgagg gaacctatac gcagcatgtc ggagcagttg
     1081 ccgtatatat cgaggggccc tctgaggcgc tttggaagat ctccggaatc gaggaaagtc
     1141 acgccggcgg cgaggctgca aaggaccttc ccgtaggaga gtatacagtg agtttctccg
     1201 acgccgacgg atgggagaag ccggaagatc agcctgtgac tgttttccga aatgtgacag
     1261 ccatggcaac cggagtctac acgcgtcacg tcggctccgt ttcaacggtc attgaagggc
     1321 cggagagcgc tttgtggaag ctgaacggcg aagggaacta ctcgagcgga gaagttgttc
     1381 aggggttgcc tgtaggcgaa tacgtagtct ccttcggcag tgttgaaggc tgggaaaaac
     1441 cggaggatca gccggtcgtc gtcacaaaag acggaatggc gtcggtctcc ggagtttact
     1501 cacagcatac gggttccgtg tccgcagtca tcgacgggcc cgagagcgca ctgtggagcg
     1561 tgaacggcga aggaaactac gcaagcggcg aggtcgttca gaagctgcct gtaggcgact
     1621 atgtcgtctc cttcggcgga gttgaaggct gggaaaaacc ggaggatcaa aaggttgtcg
     1681 tcacgaaaga cgaattgacg tcgatctccg cagtctacac ccggcaaaaa ggctccgttt
     1741 cagcagtcat tgaggggccg gagacggctt tgtggagcct gaacggcgaa ggaagctacg
     1801 cgagcggcga agtccttcaa ggcctcccgg ccggagagta catcatctct ttcagtgaag
     1861 ttgaaggctg gaaaactccg gcaaacatcg ctgtttctgt gctgaaggat tcgcttgctt
     1921 ctgttacggg gagctatgcg cagccagccg ttgaagaacc tgcggtagag gaatcagcga
     1981 caactgaaaa acctgaaaca gcggaagaac cgaaggctga agagcccgca gtcgaagaag
     2041 taaaggaaga gatcgtttcc gaggaaccga aggcagaaga ggctgtggtc gaagaagtga
     2101 aggaagaggc ggcttccgag gaacctgccg cagtggagga gccgaaagct gaagagcctg
     2161 cggagaaagc cgaagaagcg gcgacggaag cggttgctga gcannnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnggttgca gaagaaccga agcccgaaga gccggcagtc
     2341 gaagaagtga aagaagaggc ggcttccgcg gaacctgtcg cggcggagga gccgaaagct
     2401 gaagagcctg cggagaaagc cgaagaagcg gcgacggagg cggtggccga gcagtccgca
     2461 gttgccgaag aggctgcgcg ggaagttgct gcggaggttg tggaagcagt gaaggagaca
     2521 gccgaagaag ttcaggaggt tgtcaccggg gaacccgtag taacagaaga gccgaagccc
     2581 gaagagccgg tagttgaaga agtgaaggaa gaggtcgctt ccgaggaacc cgtagcagta
     2641 gaggaaccga aggcagaaga gcccgcagtc gaagaagtaa aggaagagat tgtttccgag
     2701 gaaccgaagt ccgaagaagc tgtggtcgaa gaagtgaagg ctgaacctgt ttccgtggag
     2761 cctgttgctg tggaggaacc gaagcctgaa gaacccgctg ccgaagcaga gcaggaagca
     2821 gcagtggaag aggcggcaaa agaagcggca gaggttccgg cgcctgagag ccctgccccg
     2881 gagaccgtac agcaggacaa gcaaacggaa aagccggcag gggcaacagc agctccctgc
     2941 gactgagctc cgggacactc accaaaagaa cctgttttga ttgcaacaag aagagattca
     3001 ttgaaaagcc tccgtgtttc ctgcggaggc ttcttcgtac aaaaaaaata taatagttgc
     3061 aaagttggag ctttgcctgt agactgatat tgtttgttga actgttcatc taatagagtt
     3121 ccaacatact gtaccgattt tcaggaggtg gagcaatgta cgggtattgg ggtaaactgc
     3181 ttcgggtcga cctgacacat cggacgcaca gaatcgagga tattcccgag gaggtcttcg
     3241 atctgctgct gggcggctct gcgctaggag ccaaaattct gctggaggaa actccggcga
     3301 acgtggatcc cttttcaccg gagaacaagc ttattttctc cgtcggtccg ttccaggggg
     3361 tgaatttccc ggggaacggc aagtggagcg tgatcaccaa aggtcctctt accggaacgt
     3421 acctggagtc ggccggtacc ggacactggg cgccctactt caagaagtgc ggctacgatg
     3481 cagtagtatt cgaaggaaag gcgagcagcc ctgtctatgt agtgctccgc gacgattccg
     3541 tcgagttccg cgacgcgtcc tttatctggg ggaaggacac cgtcgagacc ggcgaactga
     3601 tcaaggagga tctgggcgac aaacgtatca acgcgctcaa tatcggacct gccggggaga
     3661 tcggcaatcc catagcctgc attacctgtg acgggcacag tttcgcaggc aggggaggcg
     3721 caggcgcggt catgggttcc aagaacttga aggccgttgc cgcatggggg acgaaggaag
     3781 tccccgtttt cgacaaggag aaggcttccg agtactccaa ggaaatcttc aagctccttc
     3841 acgaacaggg ggaggggttc cgtcagcacg gaacgccggt tgtcatggtc ccgcttgaag
     3901 aaatcggcga cgtgcctatc aagtactggc gcggagacga gaataaatac ggggcctact
     3961 tgctgggagc tccccgatac accgagtatc tcaacgttaa acctctgccg tgtattaact
     4021 gtccagtcgg ctgtcatcgt cacatccatt tcgagtttcc ggacggaacg gtactcgacg
     4081 gcaacggtcc cgagtacgaa accatcggca tgatgggaac gaacctgctc atggaagacc
     4141 tgagcgccgt cgccgtcgcc aacgatattg caaaccgcat ggggatcgac accgtctcct
     4201 gcggcgcatg gatcggtttt ctcatggagt gctgggagta cggctggatc acgccgaaag
     4261 agaccgacgg cattacgatc aagtggggag atcctgaagt tctcgtcgaa ttgacgcagc
     4321 agatcgcctc catgagcggc gtaggagagt ggttccgcga gggcgtccgc ggcgcggcga
     4381 agaagatcgg caaggatgcg gagaagatca cggttgaggt gaagaatctc gactatccgg
     4441 cgcatgaccc ccgcgcagtc tacggattgg gcgtgaacta tgccgtcgga accaggggag
     4501 catgtcacga acgaggcaat ccccaggcga gcgcacttgg actcttcttc cctgaattgg
     4561 ggcaggacgc tccgtacgac cgccaggatc cgaagttcgc agcaaagacc gcgatacttc
     4621 atcaagacgt gagcgcgctg cacaactgca tgacgctctg taagttcatg atgggcggcg
     4681 cagggcttac tcttaccgag atgaatacgg ccttcaaaca tctcaccgga aaggacgttc
     4741 cggttcccga gttcgtgaag tacggcgagc gcgcctggca gcttcaacgc cttatcaacg
     4801 tccgcgacgg catgaaccgg aaggacgaca cgctcccgtt gaagatgacc atcccggcga
     4861 tggtagggcc aagggctgga aaagcgccga ctcctcacga cgaaatactg gacgattact
     4921 atgcgcagcg cggatgggac aagaacggcg tcccgacgaa ggaacgcttg gaagcgctcg
     4981 ggcttggagc atacgtgaag tatttgggcc gataggcgga gcggggaggt cgagaagatt
     5041 atgatgcagg caaaagttgg gtggagtaca gccggatcgc ccttggctgc gggaaaagag
     5101 gcggcgcagg cggcgaaggt cgacggcgcg cagatcgcgt tgctcttcgg aagcgtcgat
     5161 tacaacgccg aggagctcct gaaaggagtg cgtgaaacgc tgggcgacgt tcccgtcata
     5221 gggtgtacat cctataccgg cgttcttacc ccgggaggtt ttatcacatc tcctgacggc
     5281 tactgcgctc tgatggctct ctcggatgag gatatggcgg tttccgtcgg tgcggcggaa
     5341 aagaaagaat gcgcggttgc cgcgggagaa gaggcggctt tggccgcgct ggaagccgcc
     5401 ggtatggata tggcgcccga gttcttctac atggtcgccc ctccgggaga ggaagagttc
     5461 taccttcagg gaattcagtc cgtcatcggc agagttcctt tcttcggcgg gagcgcggcc
     5521 gacaacgacc tgtccggaaa gtggctcgtc tacggcaacg gcgtggtcaa gaacggggtc
     5581 gcggtggctt tcttctggaa caaggcgtac ggcaacaagt acacgggcgc ctacagaggc
     5641 acaggaaagc
//
LOCUS       DAOPKU010000148         5636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_15443_length_5636_cov_5.251568, whole genome shotgun sequence.
ACCESSION   DAOPKU010000148 DAOPKU010000000
VERSION     DAOPKU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15443_length_5636_cov_5.251568"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PK849_05955"
     CDS             complement(<1..1073)
                     /locus_tag="PK849_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65706.1"
                     /translation="MAYDQPYEARDGFGPMSKGMHSGLDPMYLDSFGQYAYGVNVTTR
                     GGTLKTRPGFVDLGSVGSGSNFQGAKEYLLDESDHVAFCIDGVVYVRNGETGVVTEIT
                     GHTLSETVQNVYFTQVFRWMVVQDGVSRPLVIEESGGAFSRVARDAVVDGPVPATRQA
                     PWIALSVGTIGAYAHGRYHFVPAKVPQELPALTTATDDDGEYYNNADAIPTASDESGK
                     ACFVSSDILDPLSPMSVFRMTEHRGLDEGGSYALPSELGFVHGMGGMRGAATGTGVGA
                     LFVFGSRGVSAFEVSAPRSAVVAGKSWKDIAFSQTAFYGAGTYSPFSIVNVTDDLWYV
                     DTSKHLRSVTYDRSQLSSEGYSAP"
     gene            complement(1063..1263)
                     /locus_tag="PK849_05960"
     CDS             complement(1063..1263)
                     /locus_tag="PK849_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65707.1"
                     /translation="MAEDLKAEVDKSLAYVSRQMAHAVCDLDAAIECVAREADRRLKR
                     HDKDIESLQRRVSDLEGKPDGV"
     gene            complement(1266..1631)
                     /locus_tag="PK849_05965"
     CDS             complement(1266..1631)
                     /locus_tag="PK849_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65708.1"
                     /translation="MLSANIDTLSTDVVGISARLAAEEAATASLAAVATSGLYADLAG
                     IPAERLLHVEQHTWAGGEAVPYTHALAHVPVSGSLFVLDDAVLTALAYSVSGADITYT
                     AAVPGAGVVTTYICMREGA"
     gene            complement(1807..2199)
                     /locus_tag="PK849_05970"
     CDS             complement(1807..2199)
                     /locus_tag="PK849_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65709.1"
                     /translation="MTRCCSSWTNVCPEPAPVSITNNTYNIDGDDVAFDVERFVFDGT
                     SPSNPFKTVSPGVVGEPGSYTYYVELSSTPINQGSVSVRMGGTVDQKPFLDSAGESAN
                     FQVIGKYVFFFFDLVNGDEVYVKYISTP"
     gene            complement(2322..2489)
                     /locus_tag="PK849_05975"
     CDS             complement(2322..2489)
                     /locus_tag="PK849_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65710.1"
                     /translation="MPCSGSCVFCRCVVYTSTEDCSTGCCGRPDSDTWVVAAADFEPE
                     ETTNDTVDLTD"
     gene            complement(2505..2786)
                     /locus_tag="PK849_05980"
     CDS             complement(2505..2786)
                     /locus_tag="PK849_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65711.1"
                     /translation="MANASYTNKWGQTVDTESTDTGMLPAFDRDDPGGSGIVVPNTAD
                     NAEDVATLRAKQSDGTTDVADSVGLKTPFSPQYDGSSDSTGGFPDVHAT"
     gene            complement(2842..4440)
                     /locus_tag="PK849_05985"
     CDS             complement(2842..4440)
                     /locus_tag="PK849_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65712.1"
                     /translation="MGHNITIPNVHTDVNGRLLQADARLIRVAEHIGTFLKDGQMRRP
                     NAWRDLVPYSQYEPYNGESQTTNIFRGTLGEQSGLSAWAKVEASRRGIAGTQEPVDAC
                     SYQPKTYDWAIESLSYTGYKRSWKSPVLCVRDFYAADKAKEQLAMILKAGSAVVDDTR
                     VTFQREMYMYFAALAGHFVVMTSGFNDFIDDTATRVSFDPFVDDADGDEVLTFDASLL
                     PNISTLNWDYLDYCKQWLTDMAPEGAFGMDSGMPMFMAMMDKNEFEQLVYNDANLRED
                     FRYAKPQMLIEGFNMGFKTYRGIIISHDPQQPRWALKGIDGGSATLKRVNPRRLGDAV
                     AIGQRPETNPDYLNAEFGTIIFYLKSAYTILVPRVLTSLGSGTSFGDGPAFNGEWQWV
                     NNKDMDKNILGEIGYFFARFEYHPRPEDNAANAIVLLYRRCVTPRVTRCDTDSDGTLE
                     ITTATAVSSIAAVAGDSEAFTVTLSRPAEVSVGSAVTLTDASSNTCAAYIADTVGAPV
                     YTVMTDSVGSFDATDTPVTITKTA"
     gene            complement(4636..>5636)
                     /locus_tag="PK849_05990"
     CDS             complement(4636..>5636)
                     /locus_tag="PK849_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65713.1"
                     /translation="VEQPLEEGPIDSPPEDLTGVPDHVVRKGEKSITTWKQLRAERDE
                     LERKVVDTERDRLLKEQELAELKEKLEKVPSEEELEKLKEELATTKSRLSQVDLAGTP
                     EFQEQFDKPINELFGRVVQKLMQAGHEEKEALSLARKVFRPGVTSPQSIEEVLPDESS
                     VVVGALSALLENRDPLLQKREEALKEHQQTMEALQLERNRKASMDISAQLTQVAEKAF
                     EEVGKGGSWLFVKGADEKWNEGVEARRNAALGYLRAGKPEVLARLIFEGIASPVYRKS
                     FEKVKADLDDMRKKYEAVISRRPGLEARAPARPGEDPPEAAPTTVGGIVDRVWGET"
BASE COUNT          998 a         1812 c         1725 g         1101 t
ORIGIN      
        1 ggggcggagt acccctcgga cgacaactgc gagcggtcat acgtcacgga acgcagatgc
       61 ttcgaggtgt ccacatacca caagtcgtcc gtcacgttca ctatggagaa tggactgtac
      121 gtgccggcgc cgtagaaagc ggtctgggag aacgcgatgt ccttccagct cttcccggcc
      181 acgacggcgg accgtggcgc ggagacttca aaggcgctca cgccgcggga gccgaacacg
      241 aaaagagccc cgacaccggt gcccgtggcc gccccgcgca tcccgcccat gccgtgcacg
      301 aagccaagct ccgacggcag cgcatacgat ccgccctcgt cgaggccgcg gtgctcggtc
      361 atgcggaaaa ccgacatcgg ggaaagcggg tcaaggatat ccgagctgac gaaacacgcc
      421 ttgccggact cgtcggacgc cgtcgggatc gcgtcggcgt tgttgtaata ctccccgtcg
      481 tcgtcggtcg cggtagtcag cgcggggagc tcctgcggga ccttggcggg aacaaagtgg
      541 taccggccat gggcgtaagc cccgatcgtc cctacggaca gcgcgatcca cggagcctga
      601 cgcgtggcgg gaaccggccc gtcgacgacc gcgtctctcg ccacgcggga gaacgcgccg
      661 cccgactcct cgatcacgag cggacgagag accccatcct gcacaaccat ccaccggaac
      721 acttgcgtaa agtatacgtt ttggacagtt tccgataacg tgtgccctgt gatctcggtg
      781 accacgccgg tctccccgtt gcggacgtac accaccccat cgatacagaa ggcgacgtga
      841 tcggactcgt ccaagagata ctctttggcg ccttggaaat tgctgccgga accaacggaa
      901 ccaaggtcaa caaaaccggg tcgggtcttc agtgtgccgc cgcgcgtagt gacgttgacg
      961 ccatacgcat actgcccgaa ggagtcaaga tacatggggt cgaggccgga gtgcatcccc
     1021 ttcgacatcg ggccaaaccc gtctctcgct tcgtatggtt ggtcatacgc catcgggctt
     1081 cccttccagg tcgctgactc tccgctgcag ggactcgata tccttgtcgt gccgcttcag
     1141 ccgcctgtcg gcctctcttg ccacgcactc gatagcggcg tcaagatcgc acaccgcgtg
     1201 ggccatctgg cgagagacgt aggcaagcga cttgtccacc tcggctttga gatcctcggc
     1261 catcgctatg ccccttccct catgcagatg taagtggtga cgacgccggc tccgggcacc
     1321 gccgcggtat atgtaatgtc ggccccggaa accgagtagg ccagggcggt gagcaccgca
     1381 tcatccagca caaaaaggct gccggaaacg ggaacgtggg ccagcgcatg tgtgtacggg
     1441 acggcctctc ccccggccca cgtgtgctgc tccacgtgca gaagccgctc ggccgggatt
     1501 ccggccaagt ccgcgtacag cccgcttgtg gccaccgccg ccagcgacgc agtggcggcc
     1561 tcttccgcgg ccagccgagc gcttatcccc acgacatccg tcgacaacgt gtcgatgttt
     1621 gcagacagca cgtcgatgtt cgcgttcacc tcgtcgatgc tggacgcggg cgccacggta
     1681 gagtcgattt tcgaggagtc aatgctgcct tccgggatat ctcgtgacgt caggggagac
     1741 gacaggaagc ccgcccttag ccgggcgagc tccttcgcgc tgatcgggcc tcccacgtcc
     1801 agctccttac ggtgtcgaga tatacttgac gtagacctcg tcgccattca ccaagtcgaa
     1861 gaagaaaaac acgtactttc cgatcacctg gaagttggcc gactcgccgg cggaatccag
     1921 aaacggcttc tggtccaccg tgccgcccat cctgacggag acggagccct gattgatcgg
     1981 ggtggacgag agttcgacat agtaggtata ggacccgggc tctccgacga ctcccggcga
     2041 caccgtcttg aacgggttgc tgggggacgt gccatcaaac acaaaccgct cgacgtcgaa
     2101 agcgacgtcg tcgccgtcga tgttgtacgt gttgttcgtt atcgaaaccg gtgccggctc
     2161 cgggcatacg ttagtccacg agctgcagca tcttgtcata aaaacctccg catatccata
     2221 ctatcacctt gtcactatca ggtccactaa agtaaaaggc ccccgcgccc tcatcgaacg
     2281 cggagacctg gatccgtagc gacttgggtt cgggggcgcg atcaatcggt taggtcgacg
     2341 gtgtcgttcg tagtctcctc cggttcgaaa tccgccgccg ccacgaccca agtgtcggag
     2401 tccggacggc cgcagcagcc ggtcgagcaa tcctccgtcg acgtgtagac cacgcaacga
     2461 cagaagacgc acgacccgct gcagggcatt gtccggggct ccgattaggt cgcgtggacg
     2521 tccgggaacc cgccggtcga gtcgctcgac ccgtcgtact gcgggctgaa cggcgtcttc
     2581 aggccgacgg agtcggccac gtcagtcgta ccatcggact gcttggcccg aagggtagcg
     2641 acgtcctcgg cgttgtccgc cgtgttgggg accacgatgc cgctcccgcc cgggtcgtcc
     2701 cggtcgaacg caggcagcat gccagtgtcg gtcgattcag tatcgaccgt ctggccccac
     2761 ttgttcgtgt aacttgcatt agccatgatg tcttcctttc aagtatccgg gggccctggg
     2821 ttgccagggc ccccgaggcc cttacgcagt cttggtgatg gtgacgggcg tgtccgttgc
     2881 gtcgaacgag cccacgctgt cggtcatgac cgtgtacacc ggagcaccca ccgtgtcggc
     2941 gatgtacgcc gcgcaggtgt tgctcgaggc atcggtcagg gtgaccgcgc tgcccaccga
     3001 cacttcggcc ggacgggaca gggtgaccgt gaacgcttcg gagtcgccgg cgaccgccgc
     3061 gatcgagctg accgcagtag ccgtggtgat ctcgagcgtg ccgtcactgt ccgtgtcgca
     3121 gcgcgtcacg cgaggcgtca cgcagcgccg gtacaggagg acgatcgcgt tggccgcgtt
     3181 gtcttccggg cggggatggt actcgaaccg ggcgaagaag taaccgattt cgcccaggat
     3241 gttcttgtcc atgtccttgt tgttcaccca ctgccactcg ccgttgaacg ccgggccgtc
     3301 gccgaaggac gtaccggaac caaggctcgt caggacccga ggcaccagga tcgtgtacgc
     3361 gctcttcagg tagaagatga tcgtaccgaa ctcggcgttc aggtagtcgg ggttggtctc
     3421 gggacgctgg ccgatagcca ctgcgtcgcc aaggcgccgg gggttcacgc gcttcagggt
     3481 cgcgctgccg ccgtcgatgc ccttcagggc ccagcggggc tgctgcgggt cgtgcgagat
     3541 gatgattccg cggtaggtct tgaagcccat gttgaagcct tcgatcagca tctggggctt
     3601 ggcgtagcgg aagtcttcgc gcaggttggc atcgttatac acaagctgct cgaattcgtt
     3661 cttgtccatc atcgccatga acatcggcat gccggagtcc attccgaacg cgccttcggg
     3721 ggccatgtcc gtgagccact gcttgcagta gtccaggtag tcccagttca gcgtgctgat
     3781 gttgggcagc aaagacgcat cgaaggtcag gacttcgtcg ccgtcggcgt cgtccacgaa
     3841 cgggtcaaaa gagacccggg tggcggtgtc gtcgatgaag tcgttgaacc cgctcgtcat
     3901 gacgacaaag tgaccggcca gggcggcgaa gtacatgtac atctcgcgct ggaaggtgac
     3961 tcgggtgtcg tccacgacgg cgctgccggc cttgaggatc atggccagct gctctttcgc
     4021 cttgtccgct gcataaaaat cgcgcacaca gagaacgggg gatttccacg agcgcttgta
     4081 cccggtgtag ctcagggact cgatcgccca gtcgtaggtc ttcggctggt acgagcacgc
     4141 gtccacgggc tcctgggtcc ccgcgatgcc gcgccgactg gcttccacct tcgcccacgc
     4201 gctcagaccg gactgttcgc ccagcgtgcc gcggaagatg ttagtggttt gggattcgcc
     4261 attgtacggc tcatattggc tatatggtac gaggtctctc caggcgttgg ggcggcgcat
     4321 ctggccgtcc ttgagaaacg taccgatatg ctctgccacg cgaatgaggc gagcgtccgc
     4381 ctgcaggagg cgcccgttga cgtcggtatg gacgttcggg atagtgatgt tgtgacccat
     4441 tgaggatctc ctttcttatc gaaggttgaa ttggcgacga acactcgtcg ctactcgcac
     4501 cgacgatgcg atcggtatct tcagcatcta ccggatcccc gggtgggccg ttggatcact
     4561 acggatatca gatccatact caaattaaca gaaagccccc ggaacgtcaa gtcccggggg
     4621 cgtagcgaaa tacgatcagg tctcgcccca gacccggtca acgatcccgc cgacggtcgt
     4681 aggcgcagcc tccggaggat cttctccggg tcgggcgggg gcacgagctt ccagcccggg
     4741 gcgccgactg atcaccgcct cgtacttctt gcgcatatcg tccaggtcgg ccttgacctt
     4801 ctcgaacgac ttgcggtaca caggcgatgc gatgccttcg aagatcagcc gcgccagcac
     4861 ctcaggcttg ccggccctca gatacccaag cgccgcattc ctccgcgcct ccacgccttc
     4921 gttccacttc tcgtctgcgc ccttgacaaa cagccacgag ccgcccttgc cgacttcctc
     4981 gaacgccttc tcggccacct gggtcagctg ggcgctgata tccatcgagg ctttgcggtt
     5041 tcgttcgagc tgaagcgcct ccatcgtctg ctggtgctcc ttgagcgctt cctcgcgctt
     5101 ctggagcagc gggtcgcggt tctcgagcag ggcgctcagg gcgccgacca cgacggagct
     5161 ctcgtccggc aggacctctt cgatgctctg gggcgaagtg acgccggggc ggaacacctt
     5221 acgggccagg gagagggctt ccttctcctc gtgtccggcc tgcatcagct tctgcaccac
     5281 gcggccgaag agctcgttga ttggcttgtc aaactgctcc tggaactcgg gagtcccggc
     5341 caggtcgacc tgggagagcc gggacttggt cgtcgcgagc tcttccttca gcttctccag
     5401 ctcttcttcg gacggcacct tctcgagttt ctccttcagc tcggccagct cctgctcttt
     5461 caacaggcga tctcgctccg tgtccaccac cttgcgctcg agctcgtccc gctcggcgcg
     5521 cagctgtttc cacgtggtga tcgatttttc gccttttcgc acaacatgat cgggcactcc
     5581 cgtcaggtct tccggcggcg agtcgatcgg accctcttcc aacggctgct cgaccg
//
LOCUS       DAOPKU010000149         5625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_15495_length_5625_cov_4.564093, whole genome shotgun sequence.
ACCESSION   DAOPKU010000149 DAOPKU010000000
VERSION     DAOPKU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15495_length_5625_cov_4.564093"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /gene="hemB"
                     /locus_tag="PK849_05995"
     CDS             complement(<1..973)
                     /gene="hemB"
                     /locus_tag="PK849_05995"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPE65714.1"
                     /translation="MTCTFPPRTRLRRLRENRVLAESIREVSLEPRQMILPLFVVPGS
                     GKRTPITGLHGVDHWSVDRLCEGVDEALTAGVQSFMLFGLPTEKDANGSTAWRDDQPV
                     QQALRFLKERYGRQIQLITDVCMCEYTDHGHCGFLSTDGRVDNDTSAAALGRIALSHV
                     RAGADTVAPSAMMDGQVAAIRKALDDGGFQTTPIMSYSSKFASAFYGPFREAAGSAPS
                     FGDRRGYQAPSTNWREAIRESLLDEEEGADILMVKPSLLYMDILARLREATMLPLACY
                     LVSGEYMMLRHAMAAGALDEERGLLETHFALRRAGADLIITYAAVDVA"
     gene            complement(970..2430)
                     /gene="cobA"
                     /locus_tag="PK849_06000"
     CDS             complement(970..2430)
                     /gene="cobA"
                     /locus_tag="PK849_06000"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR01469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPE65715.1"
                     /translation="MTVHLVGAGCGGPLWLTLKGKALLEGAEHIVYDSLIHPDLLQLA
                     PRACAFHPVGKRKGRPSPKQSEISALLVELGQTGKSVVRLKGGDPFVFGRGGEEALAL
                     EAAGIPWTFTPGITAALGGLGSAGIPPTHRGAADSVTLVSGHSAEGREPDADAWRALG
                     ALRGTKAIYMGASSWTKLRPLLLEGGMPPGEPCAAVAWGGWGRARLVTFSPADSPDIP
                     SPSIVALGETAGIRLSPERGPLRGLQVGLVRPSPESWATARALESLGADAYSLPLLSL
                     EELRTNGEETLIAAADWLVLTSPRGAAILLRRIDPRRISGRIAAIGEGTAAALASFGI
                     RADAVASPSTSEALAALLAARVRPGERVVFFRNEAGSPLPEEAVRSVGAFPENIAAYR
                     MIPSLPPGWESYEALWDECGLDALVFGSAALAAAWSQTAPPPRPGTALVAWGETCGRA
                     VERLFGRAPLVMQSADFDGIVAALAEVKLKTKEKTT"
     gene            complement(2427..3419)
                     /gene="hemC"
                     /locus_tag="PK849_06005"
     CDS             complement(2427..3419)
                     /gene="hemC"
                     /locus_tag="PK849_06005"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPE65716.1"
                     /translation="MKLLTRGSTLALVQTERVASSLRFLGIDVDIERVSTKGDRDTIS
                     PLCSFGGSGAFSSCIEEALLRGMGDGAVHSLKDLPSCCREGLEIAAVCPRDSVEDLLL
                     CREEQTLENLPRSAVVGTSSPRRRAQLLRLRPDLSVRELRGNVATRLQKLHDGLYDAI
                     VLAHAGLERLDIRTPNTTPLPFLPAPCQGIIALEAPLNGALFPLGERITDRETHLCSI
                     AERSLLRTLGVGCHVPFAALAELHGETMLLRAEILECDGRDSVSLTFERTVGTDEEAL
                     DAGRSLGGLFRNEPRAARLLKACAVPPPPRKEERTQRAGGPSVCTAALAGGDLR"
     gene            complement(3416..3910)
                     /locus_tag="PK849_06010"
     CDS             complement(3416..3910)
                     /locus_tag="PK849_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPE65717.1"
                     /translation="MICLDASAGPMLVVGGGATGLRKIRTLLDAGMAVTLISPEIVPE
                     LEEIVREGRVAWERRIAERRDFEEHVFALLALPRSESETVLTLAEGTRCLVNCCGAAE
                     LGTWSLAAQFRVSSSLAPVPEDTLSTAESHWYTVGVASGGRSPAGSAALKRRLQHSLE
                     GDRS"
     gene            complement(3948..5207)
                     /locus_tag="PK849_06015"
     CDS             complement(3948..5207)
                     /locus_tag="PK849_06015"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65718.1"
                     /translation="MTRLVCIGLDYTAANTAERASAWNEDRLRALKDEGRILEGLLLT
                     TCNRSEVYLRLPPDAETPSELTHPKARVYSGTELSLHLLRVLLGLESLACGESHIAAQ
                     VKACYAEGNDLCGPWLHRLFQSSLKMAKLLRTCYHPGMEPSIPRLMVDMLAPSSPPSP
                     LSVLVLGAGEMGEETSRLLLSLPLSGSPQREAKAATDGEAAPRAALKPAVGSLVLSNR
                     TPDRTEALFSRLLPRLERESGPKVATLPWEQWREASRRFDAIFFCTGSSVPLFSMEDA
                     RPGQQIFDLGSPPQVVRSAEVAGARLVAIDDLARTAEEATKRYRKKLCRLEEEATNAA
                     VSVMSELETLSGETYKRLVLHRAERIVADRAERTSKRTGTDEETLRRMGWSIVKALLS
                     PAFAESDPHARRLWKILAGELEESDDV"
     gene            complement(5204..>5625)
                     /locus_tag="PK849_06020"
     CDS             complement(5204..>5625)
                     /locus_tag="PK849_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65719.1"
                     /translation="AQAAGALLGAPYVNGVALLSLSDYLQPWSAVLRALEGAALSGLT
                     VALYNPVKRDLEEKLREVRRVFSAQGYETAYLVRDAGRDAPSVQKMPLAELDADRLDM
                     RTLILLPGSSVEEFAGLLLDRRGYRRETDKHKEESAQ"
BASE COUNT          909 a         1840 c         1973 g          903 t
ORIGIN      
        1 gggccacgtc gaccgccgcg taggtgatga tcaggtcggc tccggcgcgg cgcagggcga
       61 agtgggtttc cagcaggccg cgctcctcgt cgagcgctcc ggcggccatg gcgtggcgca
      121 gcatcatgta ctcgccgctg acgaggtagc aggcaagcgg aagcatcgtc gcctcccgca
      181 ggcgggcgag aatgtccatg tagagcagcg agggcttcac catcaaaatg tccgccccct
      241 cctcctcgtc gagaagcgac tcgcggatcg cctcccgcca gttcgtcgag ggcgcctggt
      301 atccgcggcg gtcgccgaac gagggcgcgc ttcccgcagc ctcgcggaag gggccgtaga
      361 atgccgaggc gaacttggag ctgtagctca tgatgggcgt cgtctgaaac ccgccgtcgt
      421 cgagcgcttt cctgatggcg gccacctgtc cgtccatcat cgccgaggga gcgaccgtgt
      481 cggctccggc gcggacgtgg ctcagggcga tgcgccccag cgccgcggcc gaggtgtcgt
      541 tgtcgacgcg cccgtcggtc gacagaaagc cgcagtgtcc gtggtcggtg tactcgcaca
      601 tgcagacgtc ggtgatcagc tgaatctgtc tgccgtagcg ctctttgagg aatcggagcg
      661 cctgctggac aggctggtcg tcgcgccacg ccgtgcttcc gttcgcgtcc ttttccgtgg
      721 gaaggccgaa gagcatgaac gattgcactc cggctgtgag cgcttcgtcc acaccctcgc
      781 agagccggtc cacgctccag tggtccacgc cgtgcaggcc ggtgatcgga gtccgtttgc
      841 cggagcctgg aacgacgaag agcgggagga tcatctgtct cggttcgagg gagacttcgc
      901 ggatgctctc ggcgagcacc cggttctccc ggagtcggcg cagccgcgtt cgcggcggaa
      961 atgtacaggt catgttgttt tctccttcgt tttaagtttc acttctgcca aggcggcgac
     1021 gatgccgtcg aagtccgcgc tttgcatcac gagcggcgca cgtccgaaga ggcgttccac
     1081 cgctctgccg catgtctcgc cccacgcgac gagggcggtc ccggggcgtg gcggcggcgc
     1141 ggtttggctc cacgcagcgg caagggctgc gctgccgaag acgagggcgt cgagcccgca
     1201 ctcgtcccag agcgcttcgt acgactccca gcccggcggg agcgagggga tcatcctgta
     1261 ggcggcgatg ttctccggga acgccccgac cgaccggacg gcttcctcgg gaagaggtga
     1321 tccggcctcg ttccggaaga aaacaacgcg ctcccccgga cggacgcggg cggcgagcag
     1381 cgccgcgagc gcctccgatg tcgaggggga agcgacggcg tcggcgcgga tgccgaatga
     1441 tgcaagcgca gcggccgttc cctccccgat cgcggcgatg cggcccgata tgcgcctcgg
     1501 gtcgatccgg cgcagcagga tcgccgcacc gcgggggctg gtgaggacaa gccagtcggc
     1561 tgccgcaatc aaagtttcct cgccgtttgt gcggagctcc tccagcgaaa gcagcggaag
     1621 gctgtatgcg tcggcgccaa gcgattcgag cgcgcgcgcc gtcgcccagc tctcggggga
     1681 ggggcgcacg aggccgacct ggagcccgcg cagggggcca cgttccggcg agaggcggat
     1741 tcccgctgtc tcaccaagcg caacgatgct cgggctgggg atgtccggcg agtccgcggg
     1801 agaaaaggtg acgagccggg cccgtcccca gccgccccag gcgactgctg cgcacggttc
     1861 gccggggggc ataccgccct ccagcagcag ggggcggagc ttcgtccacg acgacgctcc
     1921 catgtagatt gccttggttc cgcgaagcgc cccgagcgct ctccatgcgt cggcgtccgg
     1981 ctctctgccc tccgcgctgt gcccggaaac gagcgtgacc gagtcggccg cgcctctgtg
     2041 cgtgggcggg atgcccgcgc tccccagtcc gccgagagcg gccgtgatgc ccggagtgaa
     2101 ggtccaggga ataccggccg cttcgagcgc gagcgcttct tcgccgcccc gtccgaagac
     2161 gaaggggtcg ccgcccttga gccggacgac gctcttcccc gtttgtccca actcgacgag
     2221 gagcgcgctg atctcgctct gtttcggcga cggcctcccc ttgcgcttcc cgaccgggtg
     2281 aaaggcgcac gcccgagggg cgagctgaag gaggtcgggg tggatcaggc tgtcgtacac
     2341 gatatgctca gcgccttcaa gcagcgcttt ccccttcagc gtcagccaga gcggcccgcc
     2401 gcaccccgcg ccgacaagat ggacggtcat cggagatcgc ctccggcgag agcggcggta
     2461 cagaccgaag gccccccggc gcgttgagtg cgttcttcct tcctaggggg aggcggaacg
     2521 gcgcacgcct tcagcagacg ggccgcacgc ggttcgttcc tgaagagacc gccgagcgaa
     2581 cgcccggcgt ccagcgcctc ttcgtccgtc ccgaccgttc tctcgaaggt cagcgaaacc
     2641 gagtccctgc cgtcgcactc aaggatctcc gcgcgcagca gcatcgtctc cccgtgaagc
     2701 tccgccagcg cggcgaaggg gacgtggcag ccgacgccga gcgtccggag cagggatcgt
     2761 tcggcgatcg aacagaggtg cgtctccctg tcggtgatcc gttcacccaa cgggaagagc
     2821 gcgccgttca ggggcgcttc gagggcgatg attccctggc agggcgcggg aaggaacgga
     2881 agcggcgtcg tgttcggcgt ccggatgtct aggcgctcga gcccggcgtg cgcgaggacg
     2941 atcgcgtcgt agagtccgtc gtgcagcttc tggaggcggg tggcgacgtt cccccgaagc
     3001 tcccttacgg agaggtcggg gcggaggcgc agcagctggg cgcggcggcg ggggctcgaa
     3061 gtgcccacga ccgcgctgcg gggaaggttc tccagggtct gctcctcacg gcagagcaag
     3121 agatcctcca cggagtcgcg ggggcagacg gcggcgatct ccaacccttc gcggcagcat
     3181 gaggggaggt ccttcaggct gtgaaccgcc ccgtccccca tgccgcgcag cagcgcctcc
     3241 tcgatgcagc tcgaaaacgc tccggagccg ccgaaggagc agagggggct gatcgtgtcc
     3301 cggtcgccct tggttgagac gcgctcgatg tcgacgtcga tgccgagaaa acgaagagag
     3361 gatgcgaccc gttcggtctg aacgagggca agtgtgcttc cgcgtgtgag cagtttcatg
     3421 aacggtctcc ttccagagag tgctgcagtc ggcgcttcag cgcggcggag ccggccggag
     3481 agcgtccgcc gctggcgacg ccgactgtgt accagtggct ttcggcggta gagagcgtat
     3541 cttccggaac cggagcaagc gaagacgaaa cgcggaactg cgcggccagc gaccaggtcc
     3601 cgagctcagc ggcgccgcag cagttcacga ggcagcgcgt cccctcggcg agggtgagga
     3661 ccgtctcaga ttcggagcgg gggagcgcga gaagcgcgaa gacgtgctcc tcgaagtccc
     3721 gccgttcggc gattctccgt tcccaggcga cgcgcccctc ccgcactatc tcttccagct
     3781 ccgggacgat ctcgggagag atcagcgtta cagccattcc cgcgtcgagc agcgtccgga
     3841 ttttgcgcag tccggtcgcg ccgccgccga ccacaagcat cggtcctgcg gaggcgtcga
     3901 gacagatcat aagggaggct cgctcccggg agcgccgcga cggagcgtta cacatcgtcg
     3961 gactcctcca gctcgcccgc gaggatcttc cagagccttc gggcgtgggg gtccgactcg
     4021 gcgaaagcgg gggagaggag cgccttcacg atgctccacc ccattctgcg gagcgtctcc
     4081 tcatcggttc ccgttcgctt cgaggtccgc tcggccctgt ccgcgacgat tcgttcagcc
     4141 ctgtggagaa ccagtcgttt gtacgtctcg cccgagagcg tttcgagctc cgacatgacc
     4201 gagacagcgg cgttcgtcgc ctcctcctcc agtctgcaaa gcttcttcct gtagcgcttc
     4261 gtcgcctcct ccgccgtccg ggcgagatcg tcgatggcga caaggcgggc gccggccact
     4321 tccgcggagc gcacgacctg cgggggcgaa ccgaggtcga agatctgctg tcccggtcgc
     4381 gcatcctcca tggagaagag gggaacggag gagccggtgc agaagaagat cgcgtcgaaa
     4441 cgcctcgacg cctcgcgcca ctgttcccag ggcagcgtcg caacctttgg gccggattcc
     4501 cgctcaaggc gcggcaagag gcgggagaac aacgcttccg tgcgatcggg agtccggttc
     4561 gacaggacga gcgaaccaac agccggtttg agggcggcgc ggggcgcggc ttcgccgtcc
     4621 gtcgcagctt tggcttcccg ttgcggcgag ccggagagcg gcagtgaaag aagcagccgg
     4681 gaggtttctt ctcccatctc gcccgccccg aggacgagga cggaaagagg agaaggcggg
     4741 gaggaagggg cgagcatatc gaccatcagg cgcggaatcg agggctccat gcccggatgg
     4801 tagcaggtcc gcagcagctt tgccatcttg agagagcttt ggaacagacg atggagccac
     4861 gggccgcaga ggtcgtttcc ttctgcgtag cacgccttga cctgcgccgc gatatgcgac
     4921 tcgccgcagg cgaggctctc cagccccagg aggacgcgca gcaggtggag ggaaagctcc
     4981 gtcccggaat agacgcgagc cttcgggtgc gtcagttcgg acggcgtctc ggcgtccggc
     5041 gggaggcgca ggtagacctc cgaacggttg catgtggtca gcagaagtcc ctcaaggatc
     5101 cgcccctcgt ctttcagggc gcggagccgg tcttcgttcc atgcagaagc gcgttccgcc
     5161 gtgtttgccg cggtgtagtc gagcccgatg cagacaagcc gcgtcattgc gcggactcct
     5221 ccttgtgctt gtccgtctcc ctgcgatatc cgcgcctgtc gaggagaaga ccggcgaact
     5281 cctccacgga gcttcccgga aggaggatca gcgtgcgcat gtcgaggcgg tccgcatcga
     5341 gttcggcgag cggcatcttc tgtacggaag gagcgtcccg tcccgcgtcg cggaccaggt
     5401 acgccgtttc gtacccttgc gctgagaaga cgcgacggac ctcgcggagc ttctcttcaa
     5461 gatcgcgctt cacggggttg tagagggcga cggtgagccc ggagagggcc gctccttcga
     5521 gggcccggag gactgcgctc cacggctgga ggtagtccga cagtgagagc agcgcgacgc
     5581 cgttgacgta gggagcgccc agcagcgcgc cggcggcctg cgcag
//
LOCUS       DAOPKU010000150         5621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_15510_length_5621_cov_2.614265, whole genome shotgun sequence.
ACCESSION   DAOPKU010000150 DAOPKU010000000
VERSION     DAOPKU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15510_length_5621_cov_2.614265"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            888..1607
                     /locus_tag="PK849_06025"
     CDS             888..1607
                     /locus_tag="PK849_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65720.1"
                     /translation="MSIGDSMICLAKSRKHGGVCVAGKAVTSEKAFRWVRPVSASACG
                     ALSFGCIQPHNAYSIELLDVLQVPLKGRDTRFSFQRENYYVDPSKRWGKWEFVPFGFD
                     SLAVLEDSPESLWVDGYHSGKGLNDKVPESVTSGLTSSLYLIRPSNLRLHITEDEKKT
                     SYRASFQYHGKGYNLKITDLRFDNMLPSNVRSLNTEDTWLTISLGLPFETRTAGVHSD
                     ACRASYKLVAAVFLREFFEKR"
     gene            1616..2209
                     /locus_tag="PK849_06030"
     CDS             1616..2209
                     /locus_tag="PK849_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPE65721.1"
                     /translation="MTIFTIGHSDHSPDDFVELLLRHGIEAVADIRSAPFSRRFPAFN
                     KESLRSILAKRGIAYVFLGKELGARPADPSCYRDGRADFDLIAGSEAFRNGIARLLKG
                     ASRMRIVLLCAEKEPLHCHRCILAGRVLAEQGEDVVHILADGSAERHEETMQRLLELY
                     GKTEDDLFLSPQERVRLVCEMQEKKMAHMLKNGDEDE"
     gene            2206..2643
                     /locus_tag="PK849_06035"
     CDS             2206..2643
                     /locus_tag="PK849_06035"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPE65722.1"
                     /translation="MIKLFTIGCSGRSARDFFGALGKAQIQTVIDIRLRNTSHLAGFS
                     KMQDLPFFLESICGAAYIHIPQLAPTERILDDFKKNKGSWDEYETAYLRLLEERGAED
                     LLSPAFLHMGCLLCSETKSDYCHRRLASDYFARKFSEVSITHL"
     gene            2788..3654
                     /gene="cas1"
                     /locus_tag="PK849_06040"
     CDS             2788..3654
                     /gene="cas1"
                     /locus_tag="PK849_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HPE65723.1"
                     /translation="MSVDTPVVLNGRGAFCSYEFPSAGISDFSEIRRAQALCVLNESR
                     LFFARSIVLQKLNSLEKMLDMAEWLTRALREEIVQAAADVLGSGRVDQLMGIEGAISR
                     KIFEEMERRVPSSFGFHGRNRRPPLDPVNAYISYCNCIVYALCVPSLAKAGLNTAVGF
                     LHEPGAGRHTLALDMAEGLKPLLVDFTLATLIRHNRFEPDMTEMTPFGCFLNKTGRKA
                     AREAMINAMHWMFGIDEESSWGWPKSLWHALERYALDIAGSVLRGTPYFRWSFVDCKG
                     GSGGCTAFSSTT"
     gene            3627..3896
                     /gene="cas2"
                     /locus_tag="PK849_06045"
     CDS             3627..3896
                     /gene="cas2"
                     /locus_tag="PK849_06045"
                     /inference="COORDINATES: protein motif:HMM:NF021356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPE65724.1"
                     /translation="MHCIFFYDVNVKRVARFHKAMLRKMFWLQNSTFEGELALGEQAA
                     LRRDIVRLLNPEEDRVVMIWTPSPEAWRKTHLGIEKGNLEMVAWG"
     gene            3887..4174
                     /gene="cas2"
                     /locus_tag="PK849_06050"
     CDS             3887..4174
                     /gene="cas2"
                     /locus_tag="PK849_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPE65725.1"
                     /translation="MGMKYAEDINLIVAYDVSGRKVVKVHKMLSAELYWEQNSVFFGE
                     TAKGCPLKIQSRLRELINPAEDSVIIYQLQYPWNATVERWGKLPPWEGFVE"
     gene            4431..>5621
                     /locus_tag="PK849_06055"
     CDS             4431..>5621
                     /locus_tag="PK849_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPE65726.1"
                     /translation="MTIHQLATTALREGRVTVVEEVVQSAVSARYGTLPEKLHPAVRE
                     ALKRTYPKGLYSHQSEGIANGLEGKNVCVCTNTASGKTRIFTSVAISHLLNFPNSVVL
                     ALYPLRALLQDQAIKWREALLDAGFDGQAVQRIDGSIPMKERISRLQRGRIVLMTPDV
                     LHAWFLPSISEQEPASFLRRLSIVILDEAHIYDGIFGSNMAYLMRRLRSAAGPSSDKG
                     TPSGAAGMQFLASTATMADGEHYMKLLTGVDFYFIGPERDGTPTFEKRIVHCSISSAG
                     DGYRSFLRKIGRDVLNCDGNERFLIFTDSRKRVEELAADLQRSLRGEKEEKTEDRSAE
                     PLEEIESLIANAQILPYRAGYEDNCREKIQEALTQAKLKGVVSTSALELGIDIGNIGL
                     VILIG"
BASE COUNT         1314 a         1395 c         1562 g         1350 t
ORIGIN      
        1 actcgaaaaa tgtaaaaaag aaatatccga gagagatttg tttgtttcaa aaaaatagaa
       61 atgcccctcc ctctggggat aatttttgac acccccatta ctttggtgtg aggtaaccgt
      121 ctcccatttc aaccggagaa aattaccgtt atggttgcgc gtcgttttgt agacatctat
      181 ccctatcctg attcccgatt cctcattgct ggaattcgtc ggcttcccag acagctatct
      241 gcgattcctc tatctccgag caaatgtctg caatctcttc atcagccttt tcgcgttgtc
      301 gttcatttct gctatgtggt ttcttccatc cccagacgaa atccatcccc gcaacgattc
      361 ttaccccgaa ttccccctgt gtcataatag aagtcgcttg aattgcagaa gttcttcttc
      421 aaattcgttt gttcgtaatg tctcccgtta ggagtgaaat tatgtagcga caaatgatcc
      481 cgtggcattt tttcgactaa agatctgcct ccggcaaatt caacgtccgc gaagagagac
      541 ttttcctcca cattcagttc gatgtagcga atctgaagat cttcctctcc ttgttttttt
      601 taaaaaaaca aatctccctg cctcttgacc tcgattggat attacggtct cagagagcca
      661 ccgttccgat catgacggag ccacctctcc gatgaaagtg agccaccgtt ccggcggcat
      721 ggagccggtg ttccggcggg agagccaccg cgcttcatcc ctaagaagat gatttttcac
      781 ctctctcgta ttcttttttc accgataaac atgctaataa aaagaacaga aggacgtgag
      841 ctactcttcc ttccgtgacc aactttcttt tcaaggaggt cgggagcatg tctatcggag
      901 atagcatgat ctgtctggcg aagtccagaa agcacggcgg cgtttgcgtc gccgggaagg
      961 cagtcacttc ggagaaagca tttcgatggg ttcgtccggt cagtgcaagt gcatgcggcg
     1021 ccctttcctt cggatgcatc caaccgcata acgcctattc gatcgagctg cttgacgttc
     1081 tgcaagtccc tctgaaggga agagacaccc gcttctcctt tcagcgtgaa aactactacg
     1141 tcgatccatc gaaaagatgg ggaaagtggg agtttgttcc gttcggtttc gattcgctgg
     1201 cggttcttga ggactcgccc gagtcattgt gggtcgacgg gtatcacagt ggaaagggac
     1261 tgaacgacaa ggttccggag agcgtgacga gcggattgac atcgtcgctg tatctgatac
     1321 gcccgtcgaa tcttcgcctg catataacgg aggacgaaaa gaaaacatca taccgggcgt
     1381 cgtttcagta tcatgggaag gggtacaatc tgaagataac cgacttgcgc ttcgacaaca
     1441 tgctcccatc caacgtacga tcgctgaata ccgaggacac atggctgacg atcagcctgg
     1501 ggcttccgtt cgagacccga accgccggtg ttcattcgga cgcctgccgc gcaagttata
     1561 agcttgttgc ggcggttttt ctgcgggagt ttttcgagaa gaggtgaggc aaggcgtgac
     1621 gatattcacc ataggacatt cggatcactc gcccgatgat tttgtggagc ttttgctgcg
     1681 acacggcatc gaggcggtcg cggacattcg ctcggctcct ttcagccgcc gtttcccggc
     1741 cttcaacaaa gaatctctgc gcagtattct ggcgaagaga ggtatcgcct acgtttttct
     1801 tggaaaggag ctgggagcgc gtcccgccga cccgtcctgc tacagagacg gccgcgccga
     1861 cttcgacctg atcgctggaa gcgaagcgtt ccggaacggg atagcacggc ttctgaaagg
     1921 ggcttcccgc atgcgtatag ttcttctgtg cgcggagaag gagcccttgc actgccaccg
     1981 gtgcattctc gcggggcgag tgcttgccga acagggggag gatgttgttc atattctggc
     2041 ggacggttcg gcggagcgcc atgaagagac catgcagcgg ttgctggagc tttatggcaa
     2101 aacagaagac gatctgtttc tctctccgca ggagcgtgta cggcttgtgt gcgagatgca
     2161 ggagaagaag atggcgcaca tgctcaagaa tggggatgaa gacgaatgat caagctgttt
     2221 acgataggat gttcgggcag aagcgcccgg gattttttcg gcgcgctcgg gaaggcgcaa
     2281 atacaaaccg tgatcgatat ccgccttcgc aacacatcgc atctcgccgg tttttcaaag
     2341 atgcaggatc tgccgttctt ccttgagagc atctgcgggg ccgcgtatat tcatattccc
     2401 cagctggccc ctacggagag gatcctggat gatttcaaaa aaaacaaggg aagctgggac
     2461 gaatacgaga cggcatattt gcgcctgctt gaagaacggg gtgcggaaga cctgctctct
     2521 cccgcttttc tgcacatggg ctgtttgctc tgcagcgaga cgaagtccga ttactgtcat
     2581 cgcagactgg cttctgatta tttcgctcgg aaattttccg aagtgtcgat aacgcattta
     2641 taatacccta aagggcgcga cagtcttaaa aaacggcgca aaaagaaact cattcagcta
     2701 cacaccccgt ttttccacat tattttctcc tcgtttggca atacaacttg ttttccgaaa
     2761 taactttcat ttcgaaagga gactttcatg agcgtcgata ctcccgttgt tctgaatggg
     2821 cgaggagcat tctgctccta tgagtttccc tccgccggca tatcggattt ttctgagata
     2881 cggagagcgc aggcattgtg cgttctaaac gaaagccgcc tgtttttcgc ccggagtatt
     2941 gtattgcaga aactgaactc gcttgaaaaa atgcttgata tggctgaatg gcttactcgt
     3001 gctctgcggg aggagatcgt ccaggctgcc gctgacgtac tgggctccgg tagagtagat
     3061 cagttgatgg gtatcgaagg agcgatcagc aggaagatat tcgaggagat ggaacgccgg
     3121 gttccttcca gtttcggttt tcatggccgc aaccgtcgcc ctcctctcga tcccgtcaac
     3181 gcctacatct catactgtaa ctgcatcgta tatgcgctct gcgtcccctc gttggcaaag
     3241 gcagggctga acaccgcggt gggttttttg cacgagccgg gagctggacg gcacactctg
     3301 gctctggaca tggcggaagg gttgaagccg ttgctcgttg acttcactct ggcgacgctc
     3361 atccgccata atcgattcga accggatatg accgagatga cgcctttcgg gtgttttctg
     3421 aacaaaacgg gcagaaaagc ggcgagggaa gcgatgatca atgcgatgca ttggatgttc
     3481 gggatcgacg aggagtcgag ctgggggtgg ccgaaatctc tctggcatgc gctcgaaaga
     3541 tatgcgttgg atatcgctgg aagcgttctt cgcggaacgc cctactttcg ctggagtttt
     3601 gtggattgca aaggagggag tggcggatgc actgcatttt cttctacgac gtgaacgtaa
     3661 agcgagtcgc ccgcttccac aaggcgatgc tgcgcaagat gttctggctt caaaacagca
     3721 cgttcgaagg ggagcttgct ctgggcgaac aagcggcgct tcgccgggac atagtccgct
     3781 tgctgaaccc tgaagaagac agagtggtga tgatttggac gccttccccc gaagcctgga
     3841 gaaaaacgca tctcggtata gagaagggaa atctggagat ggtcgcatgg ggatgaaata
     3901 cgcagaggac ataaacctga tcgtcgctta cgacgtctcc gggcgcaagg ttgtaaaggt
     3961 gcataagatg ctctcggcgg agctgtactg ggaacaaaac agcgtttttt tcggggaaac
     4021 ggcaaaagga tgcccactga agatccaaag ccggttgcgg gagcttatca atcccgccga
     4081 ggacagcgtg atcatctatc agcttcagta cccatggaac gctaccgtcg aaaggtgggg
     4141 gaaactgccg ccttgggagg gattcgtcga atagactgag ttgactcttc aagaagagac
     4201 gctctccaaa ggggcgtctc ttcttgtgat ttcgggagag ggggttttgc ggtggatcat
     4261 gggaaacaca atcgtcttca gctttccaga gccgcattaa aattagcgcg cgaagtcgag
     4321 atcattaaaa gctacccgcc cggtttggta agttcatgta gtcggcagaa atcagattcc
     4381 gacggagcgg aagaagagaa tcgataacga cgacgaaggg ggagatgtcc atgacgatac
     4441 atcaacttgc aacaaccgct ctccgcgagg gacgagtcac cgtggtggaa gaggttgttc
     4501 agagcgcggt ttccgcacgt tacggcacgc ttccggaaaa gctgcacccc gcggttcgtg
     4561 aagctctgaa aaggacatat cccaaggggt tgtactctca tcagagcgaa ggcatagcca
     4621 atgggctaga gggaaagaac gtgtgcgtct gcacgaatac tgcctccgga aagaccagaa
     4681 tttttacatc cgtcgcaatc tcgcaccttc tgaatttccc aaattcagtg gtactggcgc
     4741 tctaccctct tcgggcgctg ctccaggatc aggcgatcaa gtggcgggag gcgcttttgg
     4801 atgccggttt tgacggtcag gcggtgcagc gtatcgacgg cagcatcccg atgaaggaga
     4861 ggatatcccg ccttcagagg ggaagaatag tgctgatgac ccccgacgtt ctgcacgcat
     4921 ggtttttacc ttcgatcagc gagcaggagc ccgcatcatt tcttcgaaga ctttcaatag
     4981 tgattttgga cgaggcgcac atctatgacg gcatcttcgg aagcaatatg gcgtatctta
     5041 tgcggaggct tcgctccgct gccggaccct catccgataa gggtacaccg tccggagcgg
     5101 cgggaatgca gtttttggcg agcacggcga cgatggcgga cggggagcat tatatgaagc
     5161 tgctgacggg ggtggatttt tattttattg gcccggagcg ggacggaact cccacatttg
     5221 aaaagcgcat cgtccactgc tccatttctt ccgctggtga cggctatcgt tcttttctgc
     5281 ggaagatcgg gcgcgatgta ttgaattgcg acgggaacga acgcttcttg atttttaccg
     5341 actcccggaa acgggttgaa gaactggctg cggatcttca acgcagtctc cgcggtgaaa
     5401 aagaggaaaa aaccgaagat cgctcggcag aacctcttga agagattgaa tcattgattg
     5461 caaacgcgca aatcctcccc taccgggcag ggtacgagga taactgccgc gagaagattc
     5521 aggaggcgct gacacaggca aagctgaagg gggttgtctc gacaagcgcg ctggagcttg
     5581 gcatcgacat aggcaacatc ggtcttgtta ttttaatcgg a
//
LOCUS       DAOPKU010000151         5614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_15529_length_5614_cov_4.534808, whole genome shotgun sequence.
ACCESSION   DAOPKU010000151 DAOPKU010000000
VERSION     DAOPKU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15529_length_5614_cov_4.534808"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PK849_06060"
     CDS             complement(<1..529)
                     /locus_tag="PK849_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPE65727.1"
                     /translation="MKKCFVLLAFCVLFVYSGAFAEAAEQKIRIGVIGFDSKAEGVSQ
                     EQADIVTDLFTRTLASSKTIAVYERERLAKVGEEIKLSMSGLVDMSTAIEVGKIAGVQ
                     YILMGSVTELSERMSGGAIPLFGLPVALGGGEHGAKATIDVRLIDTTTSETRLALSES
                     GTSTNSASAVSVMGLS"
     gene            complement(560..1183)
                     /locus_tag="PK849_06065"
     CDS             complement(560..1183)
                     /locus_tag="PK849_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPE65728.1"
                     /translation="MKKKLFACVLAIVCLSIFAGAALAAPLSQRPRLSVRQFDNKAGC
                     GIPAAAITEMMTTELYKSGLFNLLEREKLNYVAEEIKLGQSGLVDESTAPELGKIKGA
                     QYTMTGAVTLFYYNSTGGVVYLPGIVGGLLSRTAYVTLDIRIINTATSEVVYAAAEQG
                     AANQTAGGVVLPIVGFGSGKTGGLLATATRNSVIKHVETMKTVNWEL"
     gene            complement(1215..1733)
                     /locus_tag="PK849_06070"
     CDS             complement(1215..1733)
                     /locus_tag="PK849_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65729.1"
                     /translation="MNKWTKFFTLAVMAGLLCLFGTSAFAELPKGSIAVLTKGPSVQH
                     SAAVASILTRELLAKGHKVVDPKRLDAIRRSKAAALALEGNADAIIKLSKQYGFSTMI
                     TAQLQAGKPVLNEFQLYTGTSSLAIMVMSSGGQMIYADTVMGKQVGYTPDEAAQKSIE
                     AAAKLAVQKMLQ"
     gene            complement(1726..2235)
                     /locus_tag="PK849_06075"
     CDS             complement(1726..2235)
                     /locus_tag="PK849_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65730.1"
                     /translation="MKKQVRRLRREKAPRPFRFFLVVCVCIATAGALTAAVPALFSGN
                     GDPGAEAQLVGEENPEMPRDPGIPETVNSGLLLREQGIATAEGVGRIPEDTAQGRLLG
                     RRAALTDARRNLLVLRQKLLDGKFDVRSVSGKIAEVRIYSEKTDGALYYLKVDVPLYR
                     LMKGDVPVE"
     gene            complement(2207..3376)
                     /locus_tag="PK849_06080"
     CDS             complement(2207..3376)
                     /locus_tag="PK849_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65731.1"
                     /translation="MRIRNFFFVFLMILLSAAPAFSAGVQAAGELIKVEAEGFAPIKG
                     GDKNGAREAAKRSAYRDALEKALGAYVTGITEMQNFEVVKDKVFSQTTGIVKKFDIVR
                     EKVGDDGILYITAICHVAQAALDGVLGPAVLDALGNPRVMVLLDERIGDKRSFLSTAE
                     SETMRVFEKAGYLLVDSTQASILKNIDLDAVRESADPEVFRQLARDFQADVLIYGKAY
                     ASSFANQVVSGVRIYGVRSTVQLKAVLANTAYQLGSDAVEEKTKGVSVEDGAIKGLQP
                     AAMKASGSIVHKIAYTLISGSAGGIPGRTIKVKISNMTFKDAKELKESLQDVQGVTAV
                     YQRLFRSKRLELDVVSDMTAEEIASLLSDLGYDIEDLSSVTVEGVLNEKTGEKTP"
     gene            complement(3380..4210)
                     /locus_tag="PK849_06085"
     CDS             complement(3380..4210)
                     /locus_tag="PK849_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65732.1"
                     /translation="MKRLLACVLMMSIFLPGSISFATEVEIELHPITTEKEKGLIDWT
                     QNHIEATGMAVAPKNMKGAQAKALARRGAIVDLQRNLLEFMGGVQVDARTTMDDFMAE
                     DRVRSEVHGMIKNVELLKGEWDGEAYTVTGRIKLAEVRSIVCPRVPVDTIIIETERKT
                     PAPKASGKYTGLVIDVRHLPLIPSVTFRVFDESGRAVYGVEFANQASMLQSGICSYYN
                     NINYAQGELRVAPNPVKTKAIRLSTGNVDIVIPNSAAAKIRGSSFDFRKECKVLLVVK
                     "
     gene            complement(4669..>5614)
                     /locus_tag="PK849_06090"
     CDS             complement(4669..>5614)
                     /locus_tag="PK849_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE65733.1"
                     /translation="EVVMYDCRTRQEDMVNAARRLVEQDKVSVVIGPSGSGLCIAAAP
                     VFNRGQVPHVGTLPTNPLVTVDEKGAVRPYNFRICFLDPYQGRMIAAFAAKELGKKKA
                     ALLYDVSSDDSHGLREFFTASFKEFGGEVIADEGHREQDVDFRAQLTVIKDANPDVLV
                     IPTMGKALPLAVKQARELGIEVPIVGGDGYGDFMWEIVGDAMKDSYWVSHVDRFDPTL
                     ADFFKKYEEQTGTQCMEFMNAVMAYDSVYWVKDAIERAGSDDPVKIRDALEATKDVKL
                     MHATLTMDEFHNPKDKDGIILKADLEKQKAVFLMKLRP"
BASE COUNT         1142 a         1692 c         1458 g         1322 t
ORIGIN      
        1 acgaaagccc cattacggag acggcggaag ccgagttcgt ggatgtgccg ctttcggaaa
       61 gagcaaggcg ggtctcgctc gtcgtcgtgt cgataaggcg gacgtcgatg gtcgccttcg
      121 ctccgtgctc tccgcccccg agcgcgactg gtaggccaaa aagaggaatt gctccccccg
      181 acatcctctc gctgagttca gtcacggaac ccattaagat gtactggact cccgcaattt
      241 ttccaacttc gatcgcagtg ctcatgtcga cgagtcccga catgctcagc ttgatctcct
      301 ccccaacttt tgcaaggcgt tccctctcat agaccgcgat cgtttttgaa ctcgcaagag
      361 tacgggtaaa cagatccgtt actatatcgg cctgctcctg cgaaaccccc tcggccttgc
      421 tgtcaaagcc gatgacgccg atgcggattt tctgttccgc agcttcggcg aaagccccgg
      481 agtaaacgaa tagaacgcaa aaagccagca atacaaagca tttcttcata tttgaacccc
      541 cccgaaaaat aaaaaacatc tacagttccc agttcaccgt cttcatcgtt tcgacgtgct
      601 tgatcaccga gttgcgcgtc gcggtggcga gcaaaccgcc ggtcttgcct gatccaaagc
      661 ccactatcgg caacaccact ccccctgcgg tctgattcgc cgcgccctgc tccgctgcgg
      721 catagacaac ctcgctcgtc gcggtgttga taatgcgaat gtcgagcgtc acatacgccg
      781 tgcgcgaaag cagaccgcct acgatccccg gcaggtagac gacgcctccg gtcgagttgt
      841 agtaaaagag ggtcaccgcg cctgtcatcg tgtactgcgc gccctttatc ttaccgagtt
      901 cgggcgccgt cgattcgtcc accagtcccg actggcccag tttgatctct tccgccacat
      961 agttcagctt ctcgcgttcc agcaagttga ataatccaga cttgtacagc tcggtcgtca
     1021 tcatctcggt gatcgccgcc gccggaatac cgcaccccgc cttgttgtcg aactggcgca
     1081 cggaaagcct cggcctctgg gagaggggcg cagcgagcgc agcccccgcg aagatcgaga
     1141 ggcagacgat cgcaagaacg cacgcgaaaa gtttcttttt catgagattc tccttcgctc
     1201 cggcgcgttc cgcactattg gagcatcttt tgcaccgcga gtttcgcggc ggcctctatt
     1261 gatttctgcg cggcttcgtc cggcgtgtat ccgacctgct ttcccatcac tgtgtcggcg
     1321 tagatcatct ggccgcccga actcatcacc atgatcgcga gggacgaggt tccggtgtag
     1381 agctgaaact cgttcagaac gggctttccc gcctgaagct gggcagtgat catcgtgctg
     1441 aaaccgtact gtttgctcaa cttgatgata gcgtccgcat tcccttcgag agccagagcg
     1501 gcggccttgc tgcggcggat tgcatcaagg cgtttcggat cgactacctt gtgccccttc
     1561 gcgagaagct ccctggtcag tatggaggcg acggcggccg aatgctgaac cgacggcccc
     1621 ttcgtcaaca ctgcgatcga tcctttcgga agctccgcaa atgcgctcgt gccgaaaagg
     1681 cacagcaaac cggccattac cgcaagcgta aaaaactttg tccatttatt caaccggaac
     1741 atcccccttc atcagtctgt acaacggaac gtcgaccttc aggtagtaga gggctccgtc
     1801 cgttttctcc gaataaattc gtacttcggc gatcttccct gacaccgacc gtacatcgaa
     1861 ctttccatcg agcagttttt gccgtagcac cagcagattt cgcctcgcgt cggtcagcgc
     1921 ggcccttcgg ccgagcagcc ttccctgagc cgtatcttcc gggatgcgac cgacgccttc
     1981 cgctgtggca atcccctgct cacggaggag aagcccggaa ttcaccgttt ccggtatccc
     2041 cgggtcccgc ggcatctccg gattttcttc cccgaccaac tgcgcctccg cgcccggatc
     2101 cccgtttccc gaaaagagcg ccgggaccgc cgccgtcaac gctccggcag tcgctatgca
     2161 gacgcacacg acgaggaaaa acctgaaagg acgcggcgct ttttctctac ggagtcttct
     2221 cacctgtttt ttcattcagg acgccctcca ctgtaacgga cgacaaatct tcaatatcgt
     2281 atccaaggtc ggagagcaag gaggcgatct cctccgcagt catgtcggac acgacgtcca
     2341 attcgagtcg cttgcttctg aacagcctct gatagacggc agtcaccccc tggacatcct
     2401 ggaggctctc cttgagctct tttgcgtctt tgaacgtcat gttggagatc tttaccttga
     2461 tcgtccttcc cggtatgccg cctgcggagc cggaaatgag cgtgtaggct atcttgtgca
     2521 cgatggagcc gctcgctttc atggcggcgg gctgcaatcc cttgatcgcc ccgtcctcga
     2581 cggagacgcc cttcgtcttc tcctcgactg cgtccgatcc cagttgatac gctgtgttcg
     2641 ccagcacagc cttcagttgg accgtgcttc tcaccccata gattctgacc ccgctgacga
     2701 cttggttcgc gaaggagctg gcgtaggcct tcccgtagat caggacatcc gcctggaagt
     2761 cgcgggccag ttgtcgaaag acctccggat cagcgctttc acgaacggcg tcgaggtcga
     2821 tgttcttcag gatgctcgcc tgcgtcgaat cgacgagcaa atagccggcc ttctcgaaga
     2881 cgcgcatcgt ttcgctttcg gcggtggaga gaaaagatct tttgtcgccg attcgctcgt
     2941 cgagcagcac catcactctc gggttgccga gagcgtcgag cacggcgggc ccgagaaccc
     3001 cgtcaagagc cgcttgcgca acatggcaga tcgccgtgat gtagagtatc ccgtcgtctc
     3061 cgaccttttc cctgacgata tcgaacttct tgacgattcc cgtcgtctgc gaaaaaacct
     3121 tgtccttgac cacctcgaag ttctgcatct cggtgattcc ggtaacatac gctccaagag
     3181 ccttttcaag cgcatccctg taggcggaac gcttggcggc ttccctcgcg ccgttcttgt
     3241 cgccgccctt tatcggagcg aacccctcgg cttcgacttt gatgagctcc cccgcggcct
     3301 gaactcctgc ggagaacgca ggagcggccg acaagaggat catcagaaaa acaaaaaaga
     3361 aattccgtat acgcatgtcc tatttcacga caaggagcac tttacactct ttgcggaagt
     3421 cgaacgagct gccgcggatc ttcgccgccg ccgagttcgg gatgacgata tcgacgttcc
     3481 cggtcgaaag gcgaatcgct ttggtcttga cgggatttgg agcgacgcgg agttcgccct
     3541 gggcgtagtt gatgttgttg tagtaggagc agattcccga ctggagcatc gaggcttggt
     3601 tcgcgaactc gacgccgtag acggctctgc cgctttcgtc gaagacgcgg aaggtcaccg
     3661 agggaatcag cggaaggtgc cgtacgtcaa tgacgaggcc ggtgtacttt ccggaagcct
     3721 tgggcgcggg cgtctttcgt tcggtctcga taatgattgt atcgacggga accctggggc
     3781 atacgatgga acgcacttcg gcgagcttga tccgcccggt gacggtatag gcctccccgt
     3841 cccactcgcc cttgagaagc tccacattct tgatcatgcc gtgcacttcg gaacggactc
     3901 tgtcctcggc catgaaatcg tccatggtcg tcctcgcgtc gacctgcact ccgcccatga
     3961 actcgagcag attccgctga aggtcgacga tcgcgccgcg tcgcgccagt gccttcgcct
     4021 gtgcgccctt catgtttttc ggtgcgaccg ccattcccgt ggcttcgata tggttctgcg
     4081 tccagtcgat aagccctttt tctttttccg tggttatggg gtgcagctca atttccactt
     4141 ctgtcgcaaa agatatgctg ccgggaagaa atatactcat cataagtacg cacgcaagta
     4201 gacgcttcat agactcatcc ccctatcagt tgttcgaaca tgatgaaact actctgtcgc
     4261 agactgtcca atccctcctt ctttagatta aaattaacag acaacatgca gcatcacagg
     4321 attcgagtag aagagaatcc tcttcttttg tgtaccgaag cactattagc gaaaaaaacg
     4381 gattgcaaat tcgagcgaaa aaataaagaa ggcaaacaaa cctgttcgaa cactctgtgc
     4441 attattttct tgataaagat tgtgagggaa acacctaaga aaaacaaggt ttttgtgcat
     4501 tgattttatt tttattcatt ttgttcattg tattttttgt gtatttcact ctgtacaaac
     4561 aagccgaaga aaagaaaacc accactggtt ttgtcttttc ttcggcttgt ttggttcaac
     4621 tactacttgt tactaatttc ttcgtcttct attttgggaa aacccgccct acggtctcaa
     4681 cttcatcagg aaaacggcct tctgtttttc aagatccgct ttcagaatga tgccgtcctt
     4741 gtccttgggg ttgtggaact cgtccatggt gagcgtcgca tgcataagct tcacatcttt
     4801 cgtcgcctcg agcgcatctc gtatcttgac agggtcgtcg cttccggcgc gttcgatggc
     4861 gtccttcacc caatacacgc tgtcgtacgc cataacggcg ttcatgaact ccatgcactg
     4921 ggtgccggtc tgctcctcgt acttcttgaa gaagtcggca agtgtcgggt cgaatctgtc
     4981 gacgtggctc acccagtagc tgtctttcat tgcgtcgcca acgatctccc acatgaagtc
     5041 gccgtagccg tcgccgccga cgatcggcac ttcgataccg agttcacggg cctgcttcac
     5101 cgcgagcgga agcgccttgc ccatcgtcgg gatgaccagg acgtcggggt tggcgtcctt
     5161 gatgacggta agctgtgcac ggaagtcaac atcctgctcc cgatgtcctt cgtcggcaat
     5221 tacctctccg ccgaactcct tgaaggaggc ggtgaagaac tcgcgaagcc cgtgcgaatc
     5281 atcactggac acgtcataga gaagcgccgc tttcttcttg ccaagctctt ttgctgcgaa
     5341 tgcagcgatc atcctcccct ggtagggatc aaggaagcag atacggaagt tgtagggcct
     5401 cacggcgccc ttctcatcca cggtgacaag cgggttcgtg gggagggttc caacatgggg
     5461 aacctgtccg cggttgaaga cgggggccgc cgcgatgcac aggccgctgc cgctggggcc
     5521 gatgacgacg ctgaccttgt cctgctccac aagacgccgg gccgcgttca ccatatcctc
     5581 ctgccgtgtg cggcagtcgt acataacaac ctcg
//
LOCUS       DAOPKU010000152         5592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_15615_length_5592_cov_3.889110, whole genome shotgun sequence.
ACCESSION   DAOPKU010000152 DAOPKU010000000
VERSION     DAOPKU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15615_length_5592_cov_3.889110"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PK849_06095"
     CDS             complement(<1..380)
                     /locus_tag="PK849_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65734.1"
                     /translation="MKRACELQCDGIVFGGKKRAAHEDAFFCLFRRAWILIVCLLTLF
                     AFFPADAGAQEADPPALTDAPEGMLFRWVPPYYMTIPSGWKPMVERREGVGFFTGAHP
                     EAFEGASSEDVSMAALMVMKQTPP"
     gene            complement(413..3127)
                     /locus_tag="PK849_06100"
     CDS             complement(413..3127)
                     /locus_tag="PK849_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65735.1"
                     /translation="MRIRRLPYIVVIIASILWTFAAPSFVEAWSQQPHSQINSEAITL
                     FVRRMAGKEKFRLGPFVDNALQQPLRGVAVTSSSLTASGFVSAEAEYSMRRWIVMGGD
                     WADEPHLYSSVRHFYDPLRITGVAYLTDQSWAHGHYDSPQIDARTWGLDHPDNPFTLF
                     TAFAAYKAALEVRDDVPLPSSISAPHFKSTMNLVARDHEDQRKLHLSRAYRALGESMH
                     MLGDMTQPAHVRNDSHPADEPIEVATFPEHVRRAAANPFIDSRIRPFIASAGGTLQAP
                     AELFDQVARFTNSTFYSMDTIYDKPLGILPNNRQTPYPSPQFKDLVEVKTTVRGFVIP
                     RTVKKLYGVFDGKRVAMAQERLSFHWFDPEQTVFGGFDNLKEKAGRGIAVGANRLGPY
                     HVPHAFASSQTEVLLPLAIHACADLMDLFFPTLELKAEFEEQEEPRDAPDTEAIPKRF
                     IEGTATMEHLKKKDLAWSAYDLTIQYSGPAELLVLEKTKKIYSRKLHFNKGVLEKIET
                     LEGPLEKLPLRLFIAAEGFPLTEEEAFYEIKDGQSAVIRIDAGSRRFENVPWELKNPA
                     EVSIMPPRILVLELLEDTGDAEHEFEAVATPKGDYLFEWDFGDGSEPIEDRPRPGEKS
                     VRKHTYKGLKPGDLFSPKVILYGEKKKELAEDTISVSVIKGEKRHFFENRTFWLTGSP
                     FANDQSVVFPTVALSIVPSISGISGEIDPKSPEGKRLADMVEGMSKAGDYVKEQMIKE
                     MAKMGVSLSDSQLAEAMKQALAMGGIDFSGSAMERRPILGSGYQQAGKPVTISLSVSL
                     APIPPVPVSMPDTPRLEGRVAVKKWFFTTGSAVSREVAGSGGSASLSWTPEKGKTPES
                     FALDLVMFYTLEFYKRGTMELWLDAGAPVRYENMSVSFPVGVYFCPVR"
     gene            complement(3159..>3514)
                     /locus_tag="PK849_06105"
     CDS             complement(3159..>3514)
                     /locus_tag="PK849_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65736.1"
                     /translation="PDWKNYPDLPEEKAAIEESFDELRNALKEGDIERAVSVIDEDRR
                     EEYAALFAHKPEAMPSFAELLENAEMIFFSAAERADPATSTALRTSEYAVVLDGFTFY
                     VRWIKVDGKWVLFDF"
     assembly_gap    3515..3614
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3615..4051)
                     /locus_tag="PK849_06110"
     CDS             complement(<3615..4051)
                     /locus_tag="PK849_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65737.1"
                     /translation="MSSGKSAVPARSFRGPRVRRGGSVVLWLLFLLLAALLPVPRNEA
                     RAQTEGGREARSPSNETLRERIALLEKALSFRGEGERMEQGGDFLGALLSFKRSHTLY
                     PDPALERKIRELTAGEPSSPRPDLQPLRDRIALLEELLAGLGTL"
     gene            complement(4251..4859)
                     /locus_tag="PK849_06115"
     CDS             complement(4251..4859)
                     /locus_tag="PK849_06115"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HPE65738.1"
                     /translation="MMYWNVATEDELSESVAERLLRSLPPPVELVNRFRRRGFGYLRK
                     NAGKWRSLARTQAVLLLTDLDRAACPPGLISEWFGEHTRMPDRLLVRVAVREAESWLL
                     ADHEAMRSLLDIHSGLPDAPDTLPDPKQTLLTLARQAPREVRLGLVRQEGTRLRQALR
                     YNSLLSEWVFTEWEPERAAKRSESLRRAIHRIKERALEFSGK"
     gene            complement(4856..>5592)
                     /locus_tag="PK849_06120"
     CDS             complement(4856..>5592)
                     /locus_tag="PK849_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE65739.1"
                     /translation="SHGNAHLEQTQANARFREIAAFFSGIRYLHLVPQLLKYPERAGV
                     RSLDDDPFGAGFLEKLAGTDDSVRRARLEMIEAFLRTAVPGFDTLQFVKDERGNPRLR
                     MLHSHSGAAEGWRSEESFSDGTLRLIGLLWSLQEGGPVLLLEEPELSLNEGIAERLPE
                     VMERLRNGSSKRSQLVICTQSEALLSNPGIDGRGVIALEVGEQGSRGRVLNTQEMEAL
                     DAGFSISEAVLPRLRPKFLNRLGLKE"
BASE COUNT         1108 a         1727 c         1575 g         1082 t
ORIGIN      
        1 tttgggggcg tctgtttcat caccataagg gccgccatgg agacgtcctc cgatgacgcg
       61 ccttcgaacg cctccggatg cgcgccggtg aagaatccga ccccctccct gcgctccacc
      121 atcggtttcc agccggaagg aatggtcatg taataggggg ggacccagcg gaagagcatc
      181 ccttcgggcg cgtccgtcag ggccggcgga tcggcctcct gcgcccccgc gtccgcaggg
      241 aagaacgcaa acagcgtcag cagacagaca atcagaatcc acgcccgccg aaacagacag
      301 aagaaggcgt cctcatgcgc cgctctcttc tttccgccga agacaatacc atcgcactgc
      361 agttcgcagg ctcttttcat gactactccc tcctctttca tcatcatgct cattagcgga
      421 ccgggcagaa gtagacccct accgggaacg acacggacat attctcgtag cgcacgggcg
      481 ccccggcatc gagccaaagc tccatcgtcc cccgtttgta gaactccagc gtgtagaaca
      541 tcacgaggtc gagcgcaaag gactccggcg tctttccctt ctcgggcgtc cacgagagcg
      601 aagcgctccc gccgcttcct gcaacctccc ggctgacggc ggaacccgtc gtgaagaacc
      661 acttcttcac ggcaacacgt ccttcaagcc ttggagtatc cggcatggag accggaacgg
      721 ggggtatggg cgccagcgac acggagagcg agatcgtcac aggtttgcct gcctgttgat
      781 acccgctccc gaggatcggg cggcgctcca tcgcggaacc cgaaaaatcg attccgccca
      841 tcgccagcgc ttgcttcatc gcctcggcaa gctgtgaatc gctcagggag acgcccatct
      901 tcgccatctc cttgatcatc tgctctttga cgtagtctcc cgccttcgac atcccctcga
      961 ccatgtccgc gagacgcttc ccctccggac tcttcgggtc tatttctccg gatatcccgg
     1021 agatggacgg gacgatggag agcgcgaccg tcgggaagac cacggactgg tcgttggcaa
     1081 agggagagcc cgtcagccag aaggtacggt tctcgaagaa atgccgtttc tcccctttga
     1141 tgaccgagac ggaaatcgta tcctcggcca gctccttttt cttctcgccg tagagaatca
     1201 ccttgggaga gaacagatcc cccggcttca ggcccttgta cgtatgtttc cggacggatt
     1261 tctccccggg gcgcggcctg tcctcgatcg gctcgcttcc gtcgccgaaa tcccactcga
     1321 agagatagtc tcccttggga gtagcgacgg cctcgaactc atgctccgcg tcgcctgtat
     1381 cctcaagaag ctccagcaca aggatacgcg gaggcatgat ggaaacctcg gcagggttct
     1441 tgagctccca ggggacattt tcaaacctgc ggctccctgc gtctatccgt atgaccgcgg
     1501 actgcccgtc cttgatctcg tagaatgcct cttcttcagt gagagggaac ccctccgcgg
     1561 cgataaaaag acggagcggc agcttctcaa gggggccttc aagcgtctcg atcttctcaa
     1621 gcactccctt gttgaagtgc agctttctgc tgtaaatctt cttcgtcttc tccagaacca
     1681 gcagctcggc gggacctgaa tactgaatgg tcagatcgta cgcggaccat gcgaggtcct
     1741 tctttttcag atgctccatc gtggccgttc cttcgataaa acgcttggga atcgcttcgg
     1801 tatcgggcgc gtcgcgcggc tcctcctgct cttcaaactc cgccttcagc tccagcgtgg
     1861 gaaagaagag atccatcagg tcggcgcagg cgtggatggc cagcggaagc agcacctcgg
     1921 tctgcgaaga ggcgaaggcg tgcggcacgt gatagggtcc gagacggttc gcgcccaccg
     1981 cgatccctct ccccgctttt tccttgaggt tgtcgaaacc gccgaacacc gtctgttcag
     2041 ggtcgaacca gtgaaaactg aggcgctcct gcgccatggc gacgcgcttt ccgtcgaaca
     2101 cgccgtagag cttcttcacg gtgcgcggta tcacaaaccc ccgcaccgtc gtcttcacct
     2161 ccaccagatc cttgaactgc ggcgagggat agggcgtctg cctgttgttg ggaagtatgc
     2221 ccaggggctt gtcgtagatg gtgtccatcg agtaaaaggt cgaattcgtg aagcgggcga
     2281 cctggtcgaa gagctcggcc ggcgcctgga gcgttcctcc cgcgctcgca atgaacgggc
     2341 ggatacgcga gtcgataaac gggttcgcgg cggcgcggcg gacgtgctcg gggaaagtgg
     2401 cgacctcgat cggctcgtct gcggggtggg agtcgttccg cacgtgcgcg ggctgcgtca
     2461 tgtcgccgag catgtgcatc gactcgccga gggcgcggta cgcccttgag agatggagct
     2521 tcctctggtc ctcgtgatcg cgtgcgacga ggttcatggt cgacttgaag tgcggcgcgg
     2581 agatcgacga aggaagcggg acgtcgtcgc ggacttccag cgcggccttg tacgcggcaa
     2641 aggcagtgaa gagcgtgaac ggattatccg ggtgatccag cccccacgtt ctagcgtcga
     2701 tctgcgggct gtcgtagtgg ccatgcgccc agctctggtc ggtcaggtac gccacgcccg
     2761 ttatccgcag cgggtcgtag aagtggcgca cagacgagta gagatgcggt tcgtcggccc
     2821 agtcgcctcc catgacgatc cagcggcgca ttgaatactc cgcctccgcg gagacaaaac
     2881 cggaggccgt cagcgaagaa cttgtcactg caacgccgcg caggggctgc tgcagcgcat
     2941 tgtcgacgaa ggggccgagc cggaactttt cctttcccgc catccggcgg acgaaaagag
     3001 tgatcgcctc cgagttgatc tggctgtgcg gctgctggct ccacgcttcg acgaaggacg
     3061 gcgcggcgaa cgtccaaagg atggacgcga tgatgactac gatatacggc agtctgcgaa
     3121 tacgcacggt actttccctc ctttcccggc cggagacgtt aaaagtcgaa gagaacccac
     3181 ttgccgtcga cctttatcca ccggacatag aacgtaaaac cgtcgaggac gacagcgtac
     3241 tccgatgtgc gaagcgccgt ggaggtcgcc ggatcggcgc gctccgcggc gctgaaaaaa
     3301 atcatctccg cgttttcgag gagttcagcg aacgaaggca tggcctcggg cttgtgtgcg
     3361 aagagcgcgg cgtactcctc ccggcggtcc tcgtcgatca cggagacggc ccgttcgata
     3421 tcgccctctt tcagcgcgtt ccgtaactcg tcgaaggact cctcgatcgc cgccttctcc
     3481 tcgggcagat ccgggtagtt tttccagtcc ggatnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnagcgta ccaaggccgg caagcagctc ctcgagcagg gctatcctgt
     3661 cccgcagagg ttgcagatca gggcgcggcg aagaaggctc tcccgccgtc agctcacgga
     3721 ttttccgttc caatgccgga tcggggtaga gcgtatgact tcgtttgaat gaaagcagcg
     3781 cgccgagaaa gtcgcccccc tgctccatcc gctcgccctc gccgcggaaa gagagcgcct
     3841 tttcaagcag cgcgatacgc tcgcggagcg tctcgttcga cggagaacgc gcttcgcgtc
     3901 ccccctccgt ctgtgcacgc gcctcgtttc ggggaacggg aagaagcgcc gcgagaagaa
     3961 gaaacagcaa ccacagtacg acgcttcccc cccggcgtac gcgcggcccg cgaaacgacc
     4021 gggccggtac tgcgcttttt ccggaagaca tgatctccgc acctccttca gaacacacgg
     4081 aatgcaggca ggattccttc tgtaaaaaga ggccgcgccc gcaatttcat actgcgcttt
     4141 gttgacgcac agcttattct accaagcttg aaatgtcaaa cagggaggac tgcgatgaag
     4201 gctacaagaa aatatggatt ttcacttact tgcaaatatt tatagagctt tcacttcccg
     4261 gagaactcaa gagcgcgctc ctttattctg tgaatcgcac gccgcaaact ctcggaacgt
     4321 ttcgctgcgc gctcgggttc ccattcggta aaaacccatt ccgaaagaag gctgttgtat
     4381 cgaagcgcct gccgcagacg agttccctct tgccgcacca gaccgagacg aacctcgcgg
     4441 ggcgcctgcc gtgcaagcgt gagcaacgtc tgtttggggt cgggcagcgt atccggggca
     4501 tcgggcaatc cggaatgaat atcaagcaga gagcgcatcg cctcgtggtc cgcaagcagc
     4561 cagctctccg cctcccggac ggcgacgcgt acgagaaggc gatcgggcat tctggtgtgc
     4621 tctccgaacc attcggagat aagtcccgga ggacatgcgg cccggtccag gtccgtcaac
     4681 aacagaaccg cctgagtccg tgccaaactt cgccacttcc cggcattctt ccgaagataa
     4741 ccgaatcctc ttctccggaa tctgttgacg agctccacag gcggcgggag cgaccgaaga
     4801 agacgttccg caacggattc gctcaattca tcctcggttg ccacgttcca atacatcatt
     4861 ccttcaatcc gaggcgattc aggaattttg ggcgcaacct ggggagaacg gcctccgata
     4921 ttgaaaaccc tgcgtccagc gcttccatct cctgtgtatt caacacacgc ccccttgaac
     4981 cctgttcgcc gacttccaac gcgatcactc cgcgaccatc gatccccgga ttcgaaagca
     5041 acgcttcact ctgggtgcag atcacgagct gggaacgctt ggaactcccg tttcgaagcc
     5101 gttccatcac ttccggcagc ctttccgcaa taccctcgtt cagcgagagt tcgggctcct
     5161 ccagcaacaa cacggggccg ccctcctgca aactccaaag gaggccgata agacgaagcg
     5221 taccgtcgga aaatgactcc tcgctccgcc acccttccgc cgccccagag tgcgaatgca
     5281 gcattctcag gcggggattc cccctctcgt ccttgacgaa ttgcagcgta tcgaatccgg
     5341 gaacggccgt tcggaggaat gcttcgatca tctcaaggcg ggcgcggcga acagaatcat
     5401 ccgtgccggc aagtttttcc aggaatcctg cgccgaaagg atcgtcgtcc agagagcgta
     5461 cccctgccct ttccgggtac ttcagcagtt gcggcacaag gtgaagatag cgaatgccgg
     5521 aaaagaatgc ggcaatctca cggaaacgcg cattggcctg cgtctgctcc agatgcgcgt
     5581 ttccgtgaga tt
//
LOCUS       DAOPKU010000153         5538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_15829_length_5538_cov_4.552252, whole genome shotgun sequence.
ACCESSION   DAOPKU010000153 DAOPKU010000000
VERSION     DAOPKU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15829_length_5538_cov_4.552252"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1331)
                     /gene="gltX"
                     /locus_tag="PK849_06125"
     CDS             complement(15..1331)
                     /gene="gltX"
                     /locus_tag="PK849_06125"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPE65740.1"
                     /translation="MADTVRVRFAPSPTGALHIGGGHTALFNWLWARHTGGKFILRIE
                     DTDRERSTKEYEDTIMSGMQWLGLDWDEGPDTGGPFGPYRQSERLSTYREAAERLLDE
                     GKAYRDGEAVIFKVPQGVTLAFDDMVYGHIEMQSDTLKDVVMIKSDGMPTYNYAVVID
                     DHTMGITHVIRGEDHISNTPKQLLIYDALGWEYPTFAHLPMILGKDKKKLSKRHGATS
                     VYEYRDMGYMPDSVFNFLALLGWSPGENNEIFSRGEAVGLFDLARVIKKPSVFDMDKL
                     NYINQEHLKRLPPYERLALVEPFWREMGLPVDSHSREYLAQSLVVLCGRGQTAKEAAG
                     YSDYFLTFEPVKARYDGSEIGDEQRAVLKRFNGELLALPEWKAPVMEEFTREWSTANN
                     VKMKDIAMPLRLGITGMKVSPGVFEVAEQLGRDEVRKRLAYYNFVE"
     gene            1471..4032
                     /locus_tag="PK849_06130"
     CDS             1471..4032
                     /locus_tag="PK849_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPE65741.1"
                     /translation="MSAPDRKKLLLVDGHGLAFRAFYAVRELNAPDGTPTNAIVGFFN
                     MFQKIREDWNPDLCGVVFDAPGPTFRHKAYAEYKAGRKPTPPEFKTQLPIILELLDAM
                     GVPVVSREGVEADDVLASSACAAAAQNLETLILSSDKDILQVLDRDVVVLRPVTGISS
                     FRTMDGKGFEEEYGFSPAAMPDYLALLGDSVDNVPGVPGVGEKTAKKLLSDYGTLEGV
                     YERLDELKGALRKKMEEHREQAFRSRELTRLLCDTEEDLSVFEPRPVDAKRLLEKCRV
                     LGLKSLAVKFQLPETAEEAHEHKKDIRLREVSAEEIAASESVLLLPVIEGKYPLHLSA
                     SELLVLDETGGCRRFPGAEAASLLGRLGELSKGSVLFPDFKEAAACFGSSALSPERVR
                     DFKTAHYLLHPDKNAHLPEAVIPGYESAALAERGAAILAEYGRMERELAKYPGLSDLM
                     RTVDIPLIPVLVDMEQYGISCCSQTFGALEKDLSERLSGIEEEIASTAGERINLNSPK
                     QVAWLLFEKLGLPAGKKTKTGYSTDISVLEGLSALGRPFDEVPNLLIEYRELSKILSG
                     FVQPLTKAGEAGEGVIHGIFEPAVTGTGRLSSREPNLQNLPTFGEWSKRVKEGLQPHG
                     EDRMFVAADYSQIELRILAHLCGDKRLLDAFASGRDIHTETASWVFSTEPAFVTPELR
                     RVAKMINFGLLYGMSAFGLAQRLAIGRQEASDIVKRYFEALPGVKEYLDAGYIEAKER
                     GYTRTVAGRIRPLAEVSVSPRDRDALRRVAVNTPIQGSAADIARKAMVDFARRFPKEG
                     SVRLALQVHDSLVCECARDEAPAVESELVKIMEGAATLSVPLKVEAKKGGNLAEV"
     gene            4108..5214
                     /locus_tag="PK849_06135"
     CDS             4108..5214
                     /locus_tag="PK849_06135"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPE65742.1"
                     /translation="MRTLRSQVRWIMIAIVVLFVLSIFGMYGFDGARRGRPQSGESGD
                     YVVAEIDGKPVMRSALDQSVRNYVERMNVREIKPEDIPHLYQSTLESLVINHRLALEA
                     EQSGLKATDEELDAAVREVSEQFPTKEAFMQYLDQSGVKMAAFRENLATQIVQQKMVE
                     RASAGIAVTEEEALDFYEKTKVLFFHSPAGYTMDYARLKNEAAAAKIVEAVRQGAEWN
                     AVWSSVTSDDLVERTPESGPVFVPETAFKEQLAAFADLEPGTIGGPAQIASDDFFVGV
                     KREKVEESTVAYNEVSGDVKALLTEEKRREAQTSFFKELRGKASVIIHDFDLFPKPEA
                     PKDAAGVPVSADKAPASGPVSADQAPVSGDAEKK"
     gene            5282..5376
                     /locus_tag="PK849_06140"
     tRNA            5282..5376
                     /locus_tag="PK849_06140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5317..5319,aa:Ser,seq:gct)
BASE COUNT         1081 a         1586 c         1775 g         1096 t
ORIGIN      
        1 tcccgcgtgt gcggttactc gacgaagttg tagtaggcga gacgttttcg cacttcgtcg
       61 cgcccgagct gctccgctac ctcgaaaacg ccggggctta ccttcatccc ggtgatgccc
      121 aggcgaagcg gcatggcgat gtccttcatc ttcacgttgt tggctgtgct ccattcgcgg
      181 gtgaactcct ccatgacggg ggctttccac tcgggcagcg caagcagctc gccgttgaag
      241 cgtttgagga cggcgcgctg ttcgtcgccg atctccgaac cgtcgtatcg cgctttgacg
      301 ggttcgaagg tcaggaagta gtcggagtag cccgcggctt ccttcgccgt ctgccctctg
      361 ccgcagagca ccacgagcga ctgcgcgagg tactccctgg agtgagaatc cacgggaagg
      421 cccatctccc tccagaaggg ctcgacgagc gcaagtcgct cgtacggcgg aaggcgcttc
      481 agatgctcct ggttgatgta gttcagcttg tccatgtcga acacggacgg cttcttgata
      541 acgcgtgcga ggtcgaagag ccccacggcc tcgccgcggg agaagatctc gttgttctcg
      601 ccgggcgacc agccgagcag cgcgagaaag ttgaagaccg agtcgggcat gtaccccatg
      661 tcgcggtact cgtagacgct tgtggccccg tgccgcttgg agagcttctt cttgtccttg
      721 cccaggatca tcggaaggtg cgcgaaggtc gggtactccc agccgagcgc gtcgtagatc
      781 aagagctgct tcggcgtgtt ggaaatatgg tcctcgcccc ggatgacgtg cgtgatcccc
      841 atcgtgtggt cgtcgatgac gacggcgtag ttgtaggtgg gcatgccgtc gctcttgatc
      901 atgacaacgt ccttcagcgt gtcgctctgc atctcgatat ggccgtagac catgtcgtcg
      961 aaggcgagcg tgacgccctg agggaccttg aaaatgacgg cctctccgtc gcggtaggct
     1021 ttcccctcgt caagaagccg ctccgccgcc tcgcggtagg tcgagaggcg ttcggactgt
     1081 ctgtacggcc cgaacggacc gcccgtgtcc ggtccttcgt cccagtcgag cccgagccac
     1141 tgcatgcccg acatgatggt gtcctcgtac tcctttgtgg agcgctccct gtccgtatcc
     1201 tcgatgcgga ggatgaactt ccctcccgtg tgccgcgccc acagccagtt gaacagggcc
     1261 gtgtgcccgc cgccgatatg aagcgctccc gttgggctcg gcgcgaagcg aacccgtacc
     1321 gtgtctgcca tttcagttcc tccccgatga cgtccctcaa cctgctgatt cgggacgtgc
     1381 attgtgtgtg catgaattcc tgcaataggt tattataacc tagttgaacg cagaaaaaac
     1441 cagttggatg aaaaaggcag gcggaacgat atgagcgcac cggacagaaa gaaactactt
     1501 ctcgtggacg gacacgggct tgctttcaga gctttctacg ccgtccgcga actgaacgcg
     1561 ccggacggca cgccgacgaa cgcgatcgtc ggtttcttca atatgttcca gaagatcagg
     1621 gaagactgga accccgacct ctgcggcgtt gtcttcgacg cccccgggcc gaccttccgc
     1681 cacaaggcgt acgcggagta caaggcgggg cgaaagccga cgccgcccga gttcaaaacg
     1741 cagctcccga tcattcttga gctgctcgac gccatgggcg ttccagtggt gtcgcgtgaa
     1801 ggcgtggagg cggacgacgt gcttgcttca agcgcgtgcg ccgctgcggc gcagaacctg
     1861 gagacgctga tcctctcgtc cgacaaggac atcctccagg tgctcgacag ggacgtcgtc
     1921 gtcctcaggc cggtgacggg catcagttcc tttcgtacga tggacgggaa gggcttcgaa
     1981 gaggagtacg gcttttcccc tgcggcgatg cccgactacc ttgcgctcct cggagattcc
     2041 gtcgacaacg tacccggcgt ccccggagtg ggcgagaaga cggcgaagaa gcttctttcg
     2101 gactacggaa cgctcgaagg cgtgtacgag cgcctggatg aactgaaggg agcgctcagg
     2161 aaaaagatgg aggagcacag ggagcaggcg ttccggagca gggagctgac gcgcctcctt
     2221 tgcgataccg aggaggacct ctccgtcttc gagccacgcc ctgtggatgc gaagcggtta
     2281 ttggagaagt gccgcgtcct cgggctgaag agccttgccg tgaagttcca gttgccggag
     2341 acggcggaag aagcgcatga acacaagaag gacatacggc tccgcgaggt gtcggcggaa
     2401 gaaatagcgg cttcggagag tgtgcttctc ctccctgtga tcgaggggaa gtacccgctg
     2461 catctttcgg cctcggaact tctcgtgctc gacgaaacag ggggctgtcg gcgtttccca
     2521 ggagctgaag cggcgtccct tctcggtcgc cttggcgaac tctcgaaggg gtctgtgctc
     2581 ttcccggact tcaaagaagc cgccgcctgt ttcggctcgt cggcgctctc tccggagcga
     2641 gtgcgggatt tcaagacggc gcactacctg ctgcaccccg acaagaacgc gcacctcccg
     2701 gaggcggtca tccccggcta tgaatcggcg gcgcttgcgg agaggggagc ggcgatcctt
     2761 gcggaatacg gcagaatgga aagggagctg gcgaagtatc ccggtttgtc cgacctgatg
     2821 cgcaccgtgg atattccgct gatccccgtg cttgtggata tggagcagta cggcatctcc
     2881 tgctgttcgc agacgttcgg cgcgctggaa aaggatcttt cggagcgcct ctccggcatc
     2941 gaggaggaga ttgcgtcgac ggcgggggag cggatcaacc tgaattcccc gaaacaggtg
     3001 gcgtggctgc tcttcgagaa gctcggcctt cccgcgggga aaaagaccaa gacggggtac
     3061 tcgacggata tctccgtcct ggaggggctt tccgcactcg gtcggccctt cgacgaggtg
     3121 ccgaacctcc tcatagagta cagggagctc tcgaagattc tctccggttt tgttcagccg
     3181 ctgacgaaag ccggcgaggc gggggagggc gtgattcacg gcatcttcga gcctgcggtg
     3241 accggcaccg gaaggttgag cagcagggag ccgaacctcc agaacctccc cacgttcggc
     3301 gaatggtcga agagggtgaa ggaggggctt cagcctcacg gagaggatcg aatgttcgtc
     3361 gccgcggact attcgcagat cgaactccgt atcctcgcgc acctctgcgg cgataaacgg
     3421 ttgctggacg ccttcgcctc gggacgggac attcataccg agacggcttc gtgggtcttc
     3481 tccaccgagc ccgcgtttgt aacgccggaa ctgcgccgcg tcgccaagat gatcaacttc
     3541 ggtctgctct acggaatgag cgcgttcggc cttgcgcagc gcctcgccat agggcggcag
     3601 gaggcttcgg acatcgtcaa acgctacttc gaggcgcttc ccggagtcaa ggagtacctc
     3661 gacgcaggat atatcgaggc gaaggagcga gggtacaccc gtaccgtcgc cgggcgtatc
     3721 cgtcctctgg ccgaggtctc cgtcagtcct cgcgaccggg acgccttgcg gagagtcgcg
     3781 gtgaacaccc cgatccaggg gagcgccgcc gatattgcgc gcaaagcgat ggtcgacttc
     3841 gcgcgccgct ttccgaagga ggggagcgta cggctggcgc tccaggtgca cgactcgctc
     3901 gtctgcgaat gcgcgcgtga cgaagcgcct gcggtagagt cggagcttgt aaaaatcatg
     3961 gagggcgcgg ccactctctc cgttcccctc aaagtcgagg cgaaaaaagg aggaaatctc
     4021 gccgaggtgt aagcgaaaac gttcttctgt ggtacaattc tcccttggag tttggatatt
     4081 cgaaagagag gaagatgtcc gttgttgatg agaaccctga gatcccaggt gcggtggatc
     4141 atgattgcaa tcgtggtatt gttcgtgctc tctatttttg gtatgtacgg ttttgacgga
     4201 gcccggagag ggcgccccca gagcggtgaa agcggagatt acgttgtggc tgagatcgac
     4261 ggcaagccgg tgatgcgttc cgcgctcgat cagagcgtca ggaactacgt ggagaggatg
     4321 aacgtccggg agatcaagcc ggaggatatt ccgcacctct accagtcgac gctggaaagc
     4381 ctcgtgatca atcatcgcct ggcgctggag gcggagcagt ccggcctgaa ggcgacggat
     4441 gaagagcttg acgccgctgt cagggaagta agcgagcagt tcccgaccaa ggaggctttc
     4501 atgcagtacc tcgaccagtc aggggtaaaa atggccgctt tccgcgagaa ccttgccacg
     4561 cagattgtcc agcagaagat ggtggagcgc gcttccgccg gtatcgccgt gacggaggag
     4621 gaggcgctcg acttttatga aaagacgaaa gtgctcttct tccactctcc cgcagggtac
     4681 acgatggact acgcgaggct gaagaacgaa gccgccgccg cgaagatcgt cgaagccgtc
     4741 cgccaggggg ccgagtggaa cgccgtgtgg agttcggtga catcggacga tctggtggag
     4801 cgcacgccgg aatcaggtcc tgtgtttgtt cccgaaaccg cttttaaaga acagctcgcc
     4861 gccttcgccg atctcgaacc gggtacgatc ggcggtccgg cgcaaatcgc cagcgacgac
     4921 ttttttgtcg gggtcaagcg tgaaaaggtc gaggagtcga cggtcgccta caacgaggtg
     4981 agcggcgatg tgaaggcgct tcttacagag gagaagcgca gggaagcgca gacctctttc
     5041 ttcaaggagc ttcgcgggaa ggcgtccgtg attatccacg acttcgatct cttccccaag
     5101 ccggaggctc ccaaggatgc cgcgggcgtt cctgtatccg cagataaagc gcctgcgagc
     5161 gggcctgttt ccgccgacca ggcgcctgtg agcggagatg cggagaagaa ataatcgtct
     5221 tttcgtgctt tgcctattga cataaattcc ggccttttat agaatagagc gcgctgtttg
     5281 tggtgggttg gccgagtagg tcgaaggcgc tcgcctgcta agcgagtagg ggggcttaaa
     5341 cctccctcga gggttcgaat ccctcaccca ccgccactta cacgcagcgt ttcttttctc
     5401 cggggatatt gccaagcgca aaagaaagtg ctaaaatcct ccgggtgttc agaggagcag
     5461 ggttcctttg aacaagtacg cgtcggtagc tcagatggat agagcggcgg tctacggaac
     5521 cgcaggtcac gagttcga
//
LOCUS       DAOPKU010000154         5505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_15976_length_5505_cov_4.133028, whole genome shotgun sequence.
ACCESSION   DAOPKU010000154 DAOPKU010000000
VERSION     DAOPKU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15976_length_5505_cov_4.133028"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1225)
                     /gene="ribD"
                     /locus_tag="PK849_06145"
     CDS             complement(125..1225)
                     /gene="ribD"
                     /locus_tag="PK849_06145"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPE65743.1"
                     /translation="MTLSRKKIDEYFMRRALSLALRGMERTSPNPLVGCVVERDGVVL
                     AEGWHACCGEAHAERAALEGLDSAAGATIYVNLEPCSHFGRTPPCAPLIVEKGIRRAV
                     VGMIDPDERVSGRGLALLRDAGLDVELGVLEPECRRLNRGFLRKVRLGRPWVTLKGAV
                     GLDGSMALPSGESKWITSEGARAFAHLLRAEHDAVLVGAGTVRADDPELTVRNAWGRS
                     PLRVLLDSTLSVGPGAKILGPSCIVFSSESVSPERVAAVEASGASVVRVPRKEAGLSL
                     EAVLSELVRRGVCRLLVEGGPAVISSFLREGVADALSLFLAPRLMGQGKTFTGGLSFS
                     CMGETLALRECGVKRAGDDFLLEGVLACSPDL"
     gene            complement(1200..2690)
                     /locus_tag="PK849_06150"
     CDS             complement(1200..2690)
                     /locus_tag="PK849_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPE65744.1"
                     /translation="MAQEHIKKLLKNLPDKPGVYLMRDAEGEVLYVGKAKSLKKRVAS
                     YFRHESFASPRLRKLVASIEDISVIRTETEVEALILEAKLIKKYAPFFNVELKMGDRY
                     PYIKITSEPFPRIVVTRQRGKDDGLYFGPYVRVGDVRSLLRLIERYFPLRICAREIDP
                     ADAAKGKNRPCLHHALGRCMGVCAGLVTPAEYRERVDDVIFLLQGQSTEVVERLRKRM
                     DKAATSLQFEEAARLRDTIRAIWRITRQRVSSPLREDFDGDMWQCLNRLQELLKLPVL
                     PWRIDGFDISHMSGRETYGVVVVFEQGVPNRSLYRRFKIRTVEGVDDFRSMEETLERR
                     YGKSLEGKEPLPQLILIDGGPVQLEFARKALGKLGLEQIPSISLAKEEELIYHSTERE
                     PLRLDRGDEALRLLQRVRDESHRFAIESHRSSRSARLRRSILEDIPGIGKHRAAQLLG
                     RFGSARAIANMSAEELAAAPGIGGATAARILNFLKEQDSDPLQEED"
     gene            2805..4220
                     /gene="cysS"
                     /locus_tag="PK849_06155"
     CDS             2805..4220
                     /gene="cysS"
                     /locus_tag="PK849_06155"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPE65745.1"
                     /translation="MSLVLYNDLTRTKEPFVPLKEGHVGFYSCGPTVYDFFHIGNARP
                     FIVFDVLRRYLEYSGYKVTFVQNFTDIEDKMINRANQEGITVKQLADRFIEEYFKDAD
                     ALGIRRATHNPKATEHIPEIIALIEKLVEKGHAYAADGDVFFDVGSFPSYGVLAKQSL
                     EELQSGARVEINERKRHPLDFSLWKAKKEGEPSWPSPWGEGRPGWHIECSAMSMKYLG
                     ETLDIHSGGTDLTFPHHENEVAQAEAATGKPFVRYWIHNGYLLIDKEKMSKSLGNFLT
                     ARAALQKYPAKAIRLFMLSAHYRSPINFSEESLNQSLGAVERLENCWSDLEHARKNRK
                     TTSDGSRNEFESLFKEFEDRFVTAMDDDFNTAAALGVLFEAVKTVNTYLKETQTLEES
                     FLELAAEFFRKIDSVLGVLGLDAAAGEEDAEEIEALIAERNAARKNKDFKRSDAIRDD
                     LAARGILLEDTPDGTKWKKKA"
     gene            complement(4225..4752)
                     /locus_tag="PK849_06160"
     CDS             complement(4225..4752)
                     /locus_tag="PK849_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HPE65746.1"
                     /translation="MKVSFFKGASPKELFSELFSVRSLALSGLVAALYAVTTILFAPI
                     SYGYVQVRISEALTVLPFLFPQAVFGLFVGCLFANLFGGFGILDVVLGSAATLLAGIV
                     SSAMPTVYLAALPPVLVNMVIVGGYLSFILDLPFSLCAMYVGIGQFIACYLLGVPLTL
                     LLGKRIKAGGEDPAR"
     gene            complement(4765..5415)
                     /locus_tag="PK849_06165"
     CDS             complement(4765..5415)
                     /locus_tag="PK849_06165"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPE65747.1"
                     /translation="MIPWEKRAEEMVEKQIRARGILSERVLEAMRSVPRHLFLPEHLR
                     ESAWIDSPLPIGEEQTMSQPYMAAKMTELLAPEEGDSVLEIGTGSGYQSAVLCSMGAK
                     VTSIERIAPLAERARETLSSLGYDVSVLWSDGREKGELAGEFQGVIVTAAAPALEAWW
                     SEALAPGGRLVVPVTVLSGSERLLLREKKAPGEYVDTWHDYCRFVPLLQGRTGGYC"
BASE COUNT         1284 a         1781 c         1437 g         1003 t
ORIGIN      
        1 aagcacgctc ccgaaaccgc cacggactgg ccgcgcgtca acgctccggc aaacgacggc
       61 gcccgtacgg taagccttga gtacgttccg cttgccgaaa gcgagacaaa cgtccctaca
      121 tactctataa gtccggtgaa catgcaagca ccccctcaag cagaaaatca tcgcccgcac
      181 gcttcacgcc gcactcccgg agtgcaagag tctctcccat gcaggagaag gagagtccgc
      241 ccgtaaaggt cttcccctgc cccatcaatc ggggagcgag gaagagagaa agagcgtcgg
      301 ccactccctc tctgaggaag gaggaaatga cggcgggccc cccttcgacg agcagcctgc
      361 atacgcccct gcgcaccagc tcggagagaa ccgcttccaa cgagagaccg gcctcttttc
      421 gcggtactcg cacgacagaa gctcctgaag cctcgactgc ggcaacccgt tccggagaaa
      481 ccgactccga cgagaagacg atgcacgacg ggccaaggat cttcgctccc gggccaacag
      541 agagcgtgga atcgagcagc actctcagag gcgatcgccc ccacgcgttg cgcacggtca
      601 actcggggtc gtccgcacgg acggttccgg cgcccaccag aacagcgtcg tgttccgccc
      661 tcagaagatg agcaaaagcg cgcgccccct ccgaagtaat ccacttgctc tccccggaag
      721 gaagggccat cgaaccgtcg agcccgaccg cccccttcag cgttacccac ggtcttccca
      781 gtcggacctt ccgaagaaag ccgcgattca gccggcggca ctccggctcc aaaaccccta
      841 attcgacgtc gagtccagca tcacggagca gtgccaagcc tcggccgctg acacgctcat
      901 cgggatcgat catcccgacc acagccctcc tgataccctt ctcgacaatc agcggggcgc
      961 acggaggggt tctgccgaaa tgagaacacg gctccaggtt cacgtagatc gtcgcccccg
     1021 cagccgaatc gaggccttcg agcgccgcgc gctccgcatg cgcctcgccg cagcatgcgt
     1081 gccacccctc ggcaaggacg acgccgtccc gttccacgac gcaccccacc aaagggttgg
     1141 gcgacgttcg ctccataccc ctgagagcga ggctcaaagc ccttcgcatg aaatattcgt
     1201 caatcttctt cctggagagg gtcactgtct tgctccttta aaaagttcag aatcctggcc
     1261 gcggtcgcac cgccgattcc cggcgccgcc gccagctctt ccgccgacat gtttgcaata
     1321 gcacgcgcgc ttccgaaacg cccaagcagt tgcgccgccc ggtgcttgcc aatgccggga
     1381 atatcctcga gaatgctccg ccgcaaccgc gccgaacgcg acgaacgatg gctctcgatg
     1441 gcaaaacggt gcgactcgtc gcggactctc tgcaaaaggc ggagcgcttc gtccccccta
     1501 tccagccgca gaggttcccg ctccgtgcta tggtatatca gttcctcctc ttttgccagc
     1561 gatatcgagg gaatttgttc caatccgagc tttccgagag cctttcgcgc aaattcaagc
     1621 tgcacgggac ctccgtcgat caggatgagc tgcggcagcg gttccttgcc ttcaagcgac
     1681 ttaccgtaac ggcgttcgag cgtttcctcc atcgagcgga agtcgtccac tccctcgacg
     1741 gtcctgattt tgaaacggcg gtacagcgaa cggttaggga ctccctgttc gaacaccacc
     1801 accacgccgt acgtctctct ccccgacatg tgggagatat cgaagccgtc gatcctccac
     1861 ggaagcacag gaagcttgag cagctcctgc aaccggttca gacactgcca catatccccg
     1921 tcgaagtcct cgcgcagcgg cgaggagact ctctggcggg tgatacgcca aatcgcgcgg
     1981 atggtatcgc gcagccgcgc agcttcttca aattgcagac tcgtcgccgc cttgtccatc
     2041 ctcttccgga gacgctccac aacctcggtg gactgcccct gcagcagaaa aatgacgtcg
     2101 tcgacccgct ccctgtactc ggccggagtc accagcccgg cgcatactcc catgcagcgc
     2161 cccagcgcat gatgaagaca gggacggttc tttcccttcg ccgcatcggc cggatcgatc
     2221 tcccgggcgc atatccgcag cggaaagtac cgttcgatca ggcggagaag cgaacggaca
     2281 tcgccgaccc gcacgtaggg accgaaatag agcccgtcgt ccttacctct ctggcgcgtc
     2341 accacgattc gagggaaggg ctcggaggtg attttgatgt acgggtacct gtcccccatt
     2401 ttcagctcga cgttgaagaa gggggcgtac ttcttgatca gcttcgcctc aaggatcagc
     2461 gcctcgacct cggtctcggt ccgaatcacc gaaatgtcct cgatggaagc caccagcttc
     2521 ctcaagcggg gcgaggcgaa cgactcatgc cggaagtacg acgccacccg ttttttcagc
     2581 gacttcgcct tccccacgta gagcacctct ccctcggcgt cccgcatcag atagactccg
     2641 ggtttatccg gcagattttt caacagcttc tttatgtgct cctgcgccac gtcttcctcc
     2701 gaatttcaca tgcggctctg ttcgccgagt gaagaaaaat gttctgcatc ctaatgaatt
     2761 gtattatata atacgtgatt agaacgcatt ggaggtgctg aaaaatgagt ctggtactgt
     2821 ataacgatct gacacgcaca aaagaaccgt tcgttcctct gaaggagggg catgtgggct
     2881 tctacagttg cgggcctacg gtctacgact tcttccatat aggcaatgcg cgcccgttca
     2941 tcgtcttcga cgtcctccgc cgctaccttg agtacagcgg ctacaaagtc accttcgtcc
     3001 agaacttcac cgatatcgaa gacaagatga tcaaccgggc gaaccaggag ggcatcactg
     3061 tcaagcagct tgcggaccgt tttatcgagg agtatttcaa ggatgcggac gccctgggca
     3121 ttcgccgcgc gacgcacaat ccgaaagcga cggaacacat acccgaaata atcgcgctca
     3181 tcgaaaagct ggtcgaaaaa ggacatgcat acgctgccga cggcgacgtc ttctttgacg
     3241 tgggcagttt tccctcctac ggagtcctcg caaaacagag ccttgaggag ctccagtccg
     3301 gcgcgcgggt cgagatcaac gaacgcaaac ggcatcctct cgacttctcc ctctggaaag
     3361 cgaaaaaaga aggcgaacct tcctggccca gcccgtgggg cgagggacgc cccggctggc
     3421 acatcgaatg cagcgccatg tccatgaaat accttggaga aactctcgat attcactccg
     3481 ggggaaccga ccttactttc ccgcaccacg agaacgaagt ggcccaggcg gaggccgcca
     3541 cgggcaaacc tttcgtgcgc tactggatcc ataacggcta tctcctgatc gacaaggaga
     3601 agatgtcaaa gtcgctcggc aactttctta ctgccagagc cgcgcttcag aagtaccccg
     3661 cgaaggcaat ccgcctcttc atgctgagcg ctcactacag gtcgcccatc aacttttcgg
     3721 aggagagcct gaaccagtcg ctcggcgcgg ttgaacgtct ggagaactgc tggtccgacc
     3781 ttgagcacgc gcggaaaaac agaaaaacaa cgagcgacgg atcccggaac gagtttgaat
     3841 cactcttcaa agaattcgag gaccgttttg taacggctat ggacgacgac ttcaacaccg
     3901 cggcagccct cggcgtcctc ttcgaggccg taaagacggt caatacctat ctgaaagaaa
     3961 cacagacgct cgaagaatca ttccttgaac tggccgcgga attcttccgg aagatcgact
     4021 ccgttctggg cgttctcggg ctcgacgcag cagccggcga ggaagacgcc gaggagatcg
     4081 aggcgcttat cgcggaacgc aacgccgcac gaaagaacaa agacttcaaa agatcggacg
     4141 caatccgtga cgatctggcc gcaagaggca tcctcctgga ggataccccc gacggcacga
     4201 agtggaagaa aaaagcgtga cgactcagcg ggcggggtcc tccccgcccg cttttatgcg
     4261 cttcccaagc agcagcgtca gcggaacacc gaggagatag caggcaataa actgcccgat
     4321 cccgacatac atcgcgcaga gggaaaaggg gaggtccaaa ataaacgaaa gatacccccc
     4381 cacgataacc atgttcacaa gcacaggcgg gagcgcggct aaatagacgg tcggcatcgc
     4441 agaagagacg atccccgcca acagcgtcgc ggcgctcccc agaaccacgt cgagtatgcc
     4501 gaaaccgcca aagagattcg caaaaaggca ccccacgaaa aggccgaaaa cagcctgcgg
     4561 gaaaagaaac ggaagcacag taagcgcttc ggagattctc acttgaacat atccgtagga
     4621 aatcggggcg aacaaaatcg tcgtcaccgc gtatagagca gcgacaagcc ccgaaagcgc
     4681 aagagaacga accgaaaaca actccgaaaa caactccttc ggcgacgctc ctttaaaaaa
     4741 actgactttc accctatcac ctcttcagca atatccgccc gtcctccctt gcagaagagg
     4801 tacaaaacgg cagtaatcgt gccacgtatc cacatattcg ccgggcgcct tcttttcacg
     4861 gagaagcagc cgttcgcttc cggaaagaac cgttaccggc acaaccagcc ttcctccagg
     4921 cgccagcgcc tcggaccacc acgcctccaa tgcaggcgcg gcggctgtaa caatgacacc
     4981 ctggaactcg cctgcaagct cccccttctc ccgcccatcc gaccacagca cggaaacatc
     5041 gtaccccagg gaagaaagcg tctctctcgc cctttccgcg agcggggcga ttcgttcaat
     5101 gcttgtgacc ttcgcgccca tcgagcaaag gacggcggac tgatacccgg accccgttcc
     5161 tatctcaaga accgaatctc cttcttccgg tgcaagaagc tccgtcattt ttgccgccat
     5221 gtacggctgc gacatcgtct gttcttcgcc tatcgggaga gggctgtcaa tccacgcgct
     5281 ttcacgaagg tgttccggaa ggaaaagatg acgcgggacg gagcgcatcg cctcaagaac
     5341 tctctccgaa agaatgcccc tcgcgcggat ctgtttttcc accatctctt ccgctctttt
     5401 ctcccatgga atcacgaaat cacctccgaa atggtgtgtt ttcggattat tgtaccatgc
     5461 aaaagacgcc tccccttgaa gggaagcgtc tctttggttt acact
//
LOCUS       DAOPKU010000155         5488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16035_length_5488_cov_4.651942, whole genome shotgun sequence.
ACCESSION   DAOPKU010000155 DAOPKU010000000
VERSION     DAOPKU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16035_length_5488_cov_4.651942"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..378
                     /locus_tag="PK849_06170"
     CDS             214..378
                     /locus_tag="PK849_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65748.1"
                     /translation="MFKKLVYMAGKPFWLLPEGSLLDVEQRNGRIVDPVVFRKKNDDR
                     INENTHLSMN"
     gene            717..4439
                     /locus_tag="PK849_06175"
     CDS             717..4439
                     /locus_tag="PK849_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE65749.1"
                     /translation="MSLFNAINKTYFNAVLVFLCLLLLTFFYAYYRRTKNFSAWSTLF
                     LLVSGSMVVYLFYQGGTKGAGLLWAYIFPFLAFQFKGYKAGLRFVAVLYAVLAGVFAS
                     ALTGHAPLHFSPLFMIVYFSVHITISGFLFFYEKRRTITERIIFESREKYKALFDNFS
                     VGVAMISPEFELLELNGIARKWFDRSPEKQHSFCYEFLDSEQKLGVCEDCPLHSVMES
                     KHTATQYRKKMTVDGIRNFKIVCSPMFDKERNISAVIETLEDVTEIQRAREFLEESEN
                     RFRTLFEQVPFISVQGYDKERNVIFWNSASELLYGYPAKEALGKRLEDLIIPAEKIQE
                     HVEAISAWIQGGPPLPPSEVMLKRADGSSVHVFSSHVLIRNVRGEKEFYAIDIDITER
                     KRLEEHLSSNEEKYRLLVETAQEAICVAQDERLVYFNPTVSDMLGYSDSELSSMHFLE
                     MVYEDDRELLLQNYRKRLAGSGVEQSYEIRLLRKDGTVAWVELKATLIEWNGSPATLV
                     FLNDITTQKAALRELMETNRRLELAIERTREFASKADAANRAKSDFLANMSHEIRTPM
                     NGVLGMAGLLLDTELNREQRRCAEAVRASAESLLGVLNDILDFSKIEAGKLDLERLRF
                     DLFVMLDDFAAGMAVRAHQKKLEFICAPDPDVPSRLLGDPGRLRQILTNLVGNAIKFT
                     SKGEVVVRVSLEEESEQSALLRFSVRDTGIGVPDEIKESVFEKFIQGDSTTTRRYGGT
                     GLGLSISKQLVEMMGGRIGVESPPKLSAENGLSHWSEAGPGSDFFFTVRCEKQPSEVE
                     SGIVVQGELSGVRVLIVDDNATNREIVRAHLVSRGMRPFEAQDGASALRLLEEAVEQG
                     DPAALALVDMNMPEMDGETLGRKIREDGRFSQTRLVMLTSLADRGDAKRFAEAGFSAY
                     LPKPVRARELLGVLALVHARGEDVSPDFFATRHSLNELSPAHPRPFAGKGYRILVVED
                     NSTNRQVALGILGKFGVEACSVENGEKAVEEVGRTAYDLLLMDVQMPVMDGLEATRRI
                     RSLPPQSTGPFVPIIAMTAHAMRGDRERCLEAGMNDYISKPVTPDVLADVLERWLGTW
                     EGRHPVPEPDVSPFALPDAKGAFEETNREPDAEALIWDRNVLLERLLGDEEMVREIVH
                     GFLEDIPCQMRALEEAIRACDCVAVRERAHAIKGAAASIQAEALRAASFDVEKAGKSE
                     EADSMPSLFAEMEHCFDLLKQKMNSVEVVRREDTDS"
     gene            4420..5370
                     /locus_tag="PK849_06180"
     CDS             4420..5370
                     /locus_tag="PK849_06180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE65750.1"
                     /translation="MRILIAEDDLTSRKILAGVLRKIGHEVVETVDGLAAWAELQKTD
                     GPKLAILDWMMPEMEGLDVVREVRALDIEKPPYIIMLTTRGEKADIIAGLDAGANDYL
                     SKPFDTGELQARVEVGRRMVELQDALVESRKALAYEASHDPLTKLANRRAILEYLERQ
                     MVSASENGDILTAGMCDIDHFKRVNDTWGHQVGDDVLCELAHLLRESAEGRGAAGRMG
                     GEEFLLVLRMKAGGDYMAEYAAICAGIAATPLKTRIGEIPITVSIGVSCAKGKSGVDA
                     ILGLADAALYSAKGLGRNRVVFAGGENAGEAGAACCPRPE"
BASE COUNT         1212 a         1392 c         1649 g         1235 t
ORIGIN      
        1 gcgggtggct gttaaccact aggttcgagg ttcgagtcct cgccgaggag ccagaaagtt
       61 tccaggccat tctggatttt tccggaaggc cttttttatt tgagtcttgc cgcgcgcgta
      121 cttccctcga agagagccct taccgaagtc ttttttcggc gcgctggatg atcggaatat
      181 agaatagaag aaaagaagaa gagaggtgga tatatgttta aaaagttagt gtacatggct
      241 ggaaaaccat tctggcttct gcccgagggc agtctgctcg acgtcgaaca gcgtaacggc
      301 cgtatcgtcg atccggttgt tttccgcaag aagaacgacg acagaatcaa cgagaacact
      361 catctgagta tgaattagtc cttcccaacg ggaagtgcac ctaatacaga taggctgtac
      421 aggctccggt aatccggggc ctgtcttttt tgtctctttc gggaagtagt cggccggtcc
      481 tttcgcttcc gcaggttcgc ccggcacatc ttcgacgctc gttacgaaac aaccttctct
      541 tcaaattcat gatacaatat aataacaatt catctgttgt ttttctttgc ggctccgtat
      601 tttgaatttg attctatcga ggtcgagcta tggaccagat gcagtcgggg aagtcccgta
      661 acaatgacag gacggtacaa agtgtctccg cgcttttgtg ttccataagc gcggcgatgt
      721 cgttatttaa tgccatcaac aagacgtact tcaacgccgt gcttgtattc ctctgtcttt
      781 tgctgctgac ctttttttat gcgtactatc ggcggacgaa aaatttttca gcctggtcaa
      841 ctctgttttt attggtctcc ggctccatgg tcgtctatct cttttaccag ggagggacga
      901 aaggcgccgg tcttctttgg gcctatatct tcccgttcct cgcgtttcaa ttcaaagggt
      961 acaaggcagg cttgcgcttt gtcgctgttc tctacgcagt gctggccggc gtgttcgcgt
     1021 cggctttgac tggacatgcc cccctccact tttcccctct tttcatgatc gtctatttct
     1081 ccgttcatat aacgatctcc ggttttcttt tcttttacga aaagcggcga acaatcaccg
     1141 agcggatcat tttcgagagc agggagaagt ataaggcgct cttcgacaat ttttcggtgg
     1201 gggtggccat gatttctccg gagtttgagc tcctggagct gaacggtatc gcgagaaaat
     1261 ggtttgatcg ctctccggaa aagcagcatt ctttttgcta cgaatttctg gattcggagc
     1321 aaaaactcgg cgtctgcgaa gactgtcctc tgcactcggt aatggagtcg aaacatacgg
     1381 cgacgcagta caggaaaaag atgacagtag acggcatccg taatttcaag atagtctgca
     1441 gcccgatgtt cgacaaggaa aggaatattt ccgcagttat cgaaactctc gaggacgtta
     1501 ccgaaattca acgggcgcgc gaattccttg aggagagcga gaacaggttc cgcactcttt
     1561 ttgaacaggt cccatttatt tccgtacagg ggtacgacaa agagagaaac gtgatcttct
     1621 ggaacagcgc gagcgagctc ctctacgggt accctgcgaa agaggctctt ggaaaacgac
     1681 tggaggatct cattataccc gccgaaaaaa tacaggagca tgtcgaagcc atctcggcat
     1741 ggatacaagg aggccctcct cttcctccct ccgaggtcat gctgaagcgg gctgacggca
     1801 gctccgtgca tgtcttctcc agtcacgtgc tgatccggaa tgtacgagga gagaaggagt
     1861 tctacgctat tgatattgac ataaccgaac ggaagcgtct tgaggagcat ttgagttcga
     1921 atgaggagaa gtaccgtctt ctggttgaaa ccgcccagga ggccatctgt gtcgcccagg
     1981 acgaacgact ggtctacttc aaccccacgg tgagcgatat gctcgggtat tccgacagcg
     2041 agctttcttc gatgcacttt cttgaaatgg tctatgaaga cgacagggag ctgctgctgc
     2101 aaaactatcg aaagcgcctt gcagggagcg gcgttgagca gagctacgaa atccgtctgt
     2161 tgcgaaagga cggcacagtg gcgtgggtgg agctgaaagc gaccctcatc gagtggaacg
     2221 gcagcccggc cacgcttgtc ttcctcaacg acatcacgac gcagaaagcc gctctgagag
     2281 agcttatgga gacgaatcgc aggctggagc ttgccatcga acgaacaagg gagttcgcct
     2341 caaaggccga tgcggcgaat cgggcgaaaa gcgattttct tgccaatatg agccacgaaa
     2401 tacgcacgcc gatgaacggc gttctcggga tggccggttt gttgctggat accgagctga
     2461 accgagagca gcggcggtgt gcagaggctg tgcgcgcaag cgccgaatcg cttctcggcg
     2521 tgctcaacga tatccttgat ttctccaaga ttgaggccgg caagcttgat cttgagcgcc
     2581 ttcgattcga tcttttcgtt atgcttgacg attttgccgc cggcatggct gttcgtgcgc
     2641 accaaaagaa gctcgagttc atctgtgcgc cggacccgga cgttccatcc cgcctcttgg
     2701 gcgatccggg gcgtctccgc cagattctta cgaacctcgt tggcaacgcc atcaagttta
     2761 catccaaagg ggaggttgtc gtgcgcgttt ccctggagga ggagagcgaa cagagcgcgc
     2821 ttctccggtt ttctgtgagg gatactggaa tcggcgtgcc cgatgaaata aaggagagcg
     2881 tctttgaaaa gtttatccag ggagactcca cgacaacccg ccgttacggg ggcacagggc
     2941 tcgggctttc catctcgaaa cagctggttg agatgatggg aggaaggatc ggcgtggaaa
     3001 gccccccgaa gctttctgct gagaacggtc tttcccattg gagcgaggcg gggcccgggt
     3061 ccgacttctt ttttacggtc cgatgcgaaa aacagccttc tgaagtggag tccggtattg
     3121 tcgtacaggg agagctgagc ggagttcggg tgttgatcgt tgacgacaac gcaaccaatc
     3181 gggagatcgt ccgcgcgcat ctcgtctccc gcggtatgcg tcctttcgag gcgcaggatg
     3241 gagcctccgc cctccggttg cttgaggagg ctgttgagca gggcgatccg gccgctctcg
     3301 ccctcgtcga tatgaacatg ccggaaatgg acggcgaaac gctgggaaga aaaatccgtg
     3361 aggatgggcg tttctcacag acgcggctgg ttatgctcac ctcgcttgcg gacagaggag
     3421 acgccaaacg ttttgcggag gccggatttt ccgcctacct tcccaaacct gtccgtgccc
     3481 gcgaactcct tggagtactt gcgttggtgc atgccagggg agaagatgtc tctccggact
     3541 ttttcgccac acgtcactcg ctgaacgaac tttcaccggc gcacccccgg ccttttgccg
     3601 ggaaggggta ccgtatcctc gtcgtggagg ataattccac caaccggcag gttgcgctcg
     3661 ggatactcgg aaagttcggc gtagaggcct gttccgtcga gaacggagaa aaggccgtcg
     3721 aggaggtcgg cagaacagcg tacgatctgc tccttatgga tgtgcagatg cccgtgatgg
     3781 acggacttga ggctacccgg cggattcgtt cccttcctcc gcagagcacc ggtcctttcg
     3841 tgccgatcat cgcgatgaca gcgcatgcaa tgcgtggaga cagagaacga tgtcttgaag
     3901 ccgggatgaa cgactatatc tccaagccgg tgacgcccga cgtacttgcg gatgtccttg
     3961 aacgctggct tggtacatgg gaagggcgcc atcccgttcc agagccggat gtatcgccgt
     4021 tcgcgctccc cgatgcgaag ggcgccttcg aagaaaccaa cagggaacct gacgcggagg
     4081 ctctgatctg ggacagaaac gtcctccttg agcgcttgct gggggacgaa gagatggttc
     4141 gggagatagt gcacggcttt cttgaagata ttccgtgcca gatgagagcg cttgaagagg
     4201 cgataagggc ctgcgattgc gttgcagtga gggagcgggc gcatgctata aagggagcgg
     4261 cggcgagtat tcaggccgaa gctctgcggg ctgcgtcgtt cgatgtggaa aaggccggga
     4321 agtcggaaga ggcggattcg atgccttctc tcttcgcgga gatggagcat tgtttcgatc
     4381 tcttgaagca aaagatgaac tccgtggagg tagtgcgccg tgaggatact gatagctgaa
     4441 gacgatctga catctcgcaa aattctcgca ggagtgctcc gtaagatcgg ccacgaggtc
     4501 gtggagacgg tggatggttt agccgcatgg gcggagctgc aaaaaacaga cggcccgaag
     4561 ctggcgatcc tcgactggat gatgccggag atggaggggc tggatgtcgt acgagaggtg
     4621 cgcgccctgg atatcgaaaa gccgccgtat atcatcatgc tgaccaccag gggcgagaag
     4681 gcggacatta ttgccggcct ggacgccgga gcgaacgatt atctctcgaa gccgttcgat
     4741 accggtgaac tgcaggcgcg cgtcgaggtg gggcgccgca tggtcgaact tcaggacgcg
     4801 cttgttgaaa gcagaaaggc gcttgcctac gaagcctccc atgatccgct gaccaaactg
     4861 gccaaccggc gggctattct ggagtacctg gagcggcaga tggtctccgc gagtgagaac
     4921 ggtgatatcc tgacggcggg aatgtgcgat atcgaccatt tcaagagagt gaacgatacg
     4981 tgggggcatc aggtgggcga cgatgttttg tgcgagctcg cccatcttct gcgcgaaagc
     5041 gccgagggga gaggggccgc aggccgtatg ggcggggagg aatttctgct tgtcctccgg
     5101 atgaaagcgg ggggcgatta catggcggaa tacgccgcga tctgcgccgg gatcgcggcg
     5161 acgcctctga agacgagaat aggggaaatt ccgatcacag tcagtatcgg cgtttcatgt
     5221 gcaaaaggga agagcggagt ggacgccatt ctgggtctgg cggacgccgc cctctacagc
     5281 gcgaagggcc tcggacggaa cagagtcgta ttcgccgggg gggaaaacgc cggagaagcc
     5341 ggcgccgctt gttgtcctcg cccggaataa ggccgccgga gaaagagaag agccttcccg
     5401 tacggaaagg ctctccagat gctagaccat ttcaacctct tcgtcagggt cgaagaggag
     5461 gatatcccac cgttcttcgg tcagttca
//
LOCUS       DAOPKU010000156         5471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16091_length_5471_cov_7.504247, whole genome shotgun sequence.
ACCESSION   DAOPKU010000156 DAOPKU010000000
VERSION     DAOPKU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16091_length_5471_cov_7.504247"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..367)
                     /locus_tag="PK849_06185"
     CDS             complement(104..367)
                     /locus_tag="PK849_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HPE65751.1"
                     /translation="MSMESAVAFYERVKKDVELQKKLKELGSKEKIQSYVKNDLGYDF
                     TKEEMQSVLLEQNPEMTDEDLEAAVGGAGCTPFDGIFIAAVYA"
     gene            complement(448..1899)
                     /locus_tag="PK849_06190"
     CDS             complement(448..1899)
                     /locus_tag="PK849_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPE65752.1"
                     /translation="MCMESAASLFGCAAPGKSLLPPGDTACFSRDRAGVEPLLYAGLE
                     SEIVAGKTYEALFAAGVPRKWRSEALVDFLATGTCVFPGGKTFFEGVFEVPPGHDLIV
                     SEGSASVVEREPLPEASGELGEETLPLLRDLLEEALGDGLETAALCLSGGLDSSSLAA
                     AWAKRGTPRCFVYGPPGAPDRERALEAAKSLGIDPVLLEPDGPAKLDELKELFRILEI
                     PVHIPGAPLPQFRLIRAMAGDGVKTVLSGQGGDELLCGYPWHFPLAMKKLRSGDPEAA
                     ARLEALHEKNPPFGPIELRMTRRCFTRTSSWVVLNDGGACAALGLSHEEAAEREGVRF
                     FGADLDDWDALRRHGLTRRSLRYLLHYDYHLARHFRVEGRAPMLSADVVDLVSRFRLN
                     FLYGEGLLKYPLRLLFPELPESIRFEARKTGFWHNGPSLPDFRGEVRRMLETTLLGDL
                     VIRPEMVEKMTPAALWRFFSAGTLLEDDTLIGG"
     gene            2501..2749
                     /locus_tag="PK849_06195"
     CDS             2501..2749
                     /locus_tag="PK849_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65753.1"
                     /translation="MEKHVSIVLRFSDTQADSIRKSAMIFIIQKLRLPVFCRLIYKLF
                     SALAGEPAEGTTGAKFPVSKGLSVSEMPNSKLRRGTLS"
     gene            2746..3192
                     /locus_tag="PK849_06200"
     CDS             2746..3192
                     /locus_tag="PK849_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65754.1"
                     /translation="MKAVRHVLRNFSFRQAVPFAVAGIVVLLALFYMFPVVPDRDEDE
                     FRAMEIIKGLRAVRAAAHMFFEKNPDVPAGPLHIDSLKPYTNADISLFLIERNAQGDW
                     YCSFGHEISGNVRKKLRARASEYELLKNASDQYDGGPVVLMAVRKF"
     gene            3335..>3902
                     /locus_tag="PK849_06205"
     CDS             3335..>3902
                     /locus_tag="PK849_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HPE65755.1"
                     /translation="MIIYRNAVPDDLAGVAELQKRYHISTIREEDKADGFVTTLFTPE
                     QFLSLMKDENGLAVACDGNKIISYAMAASWEYWSAWPLFRHMIADLPNIEYLGRVLST
                     ENSYQYGPVCIAGEYRGRGVLEHIFEFSRRQMVSRYPVMVTFINRINPRSYDAHTRKV
                     GMELIKTFDFNGNHYYELGYDMTRPVPGT"
     assembly_gap    3903..4002
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4154..4495)
                     /locus_tag="PK849_06210"
     CDS             complement(4154..4495)
                     /locus_tag="PK849_06210"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE65756.1"
                     /translation="MKTFDMETVEKFTGRPGFIGKFIHSEGFTFAQWEIKKGTELVFH
                     DHPHEQTTLLLAGKMELGTADNKVTLTPGQGIVFAGGEAHGGFALEDCLVIDVFCPVR
                     EDFKKLQQEQK"
     gene            4885..>5471
                     /gene="thrC"
                     /locus_tag="PK849_06215"
     CDS             4885..>5471
                     /gene="thrC"
                     /locus_tag="PK849_06215"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPE65757.1"
                     /translation="MRKGILHSYERFFPLTDRTPRLSLCEGDTPLVPLRNIGRELGVE
                     LYGKYDGLNPTGSFKDRGMVLAVSKALEEGAKALICASTGNTSASAAAYGAAAGIPCF
                     VLLPAGKVAVGKLAQALIYGARVIAVRGNFDRALEMAREASRERGSLAIVNSVNPYRL
                     FGQRSASWEICDELGGAPDWLVLPVGNAGNISAYCA"
BASE COUNT         1148 a         1529 c         1529 g         1165 t
ORIGIN      
        1 aaccggcggt tgttctctct tggcttctcc tgccgtttcc cctttcatcc attcacttcc
       61 cttccaggaa tacctccgct ctcccatacc cctccgcaac cagtcaggcg tagacagcgg
      121 cgatgaatat gccgtcgaat ggcgtacaac cggctcctcc gacggccgcc tccagatctt
      181 cgtctgtcat ctcgggattt tgctcgagga gcaccgactg catctcctct ttggtgaaat
      241 cgtagcccag atcgtttttt acgtatgatt ggatcttctc cttgcttcca agctccttca
      301 gcttcttctg caactccaca tccttcttca cccgctcgta gaacgcaacc gccgattcca
      361 tggacatata cagcacctcc gtatgttctc tttctttctg gaagatcttc tattcgtttc
      421 aatccgcttc ttctctctgt gtcacaatca cccaccgatc agcgtgtcat cctccaggag
      481 cgtccccgcg gagaagaagc gccaaagggc agcgggggtc atcttctcca ccatttccgg
      541 acggatgacc agatcgccga ggagcgtcgt ctccagcatg cgccgcactt cgccacggaa
      601 atccggaagg gaaggtccgt tatgccagaa ccccgtcttg cgggcctcga agcgaatact
      661 ttccggaagc tctggaaaga gcaggcgcag cgggtatttg agcaatccct ctccgtagag
      721 aaagttcagc cggaagcgcg agaccagatc gaccacgtcc gcgctcagca tgggagctcg
      781 cccctcgacc ctaaaatggc gcgccagatg gtagtcatag tgcagaaggt agcgcagact
      841 cctccgggtc aggccgtgcc gtcgcagcgc gtcccagtcg tccaggtccg cgccgaagaa
      901 gcgcacgcct tcccgttcag cggcctcctc atgcgagagg ccaagtgcag cgcacgcccc
      961 tccgtcgttc aggacgaccc agctggatgt ccgggtgaag cagcgccgtg tcatcctcag
     1021 ttcgatggga ccgaaggggg gattcttctc gtggagcgct tcgagccgtg ccgcagcctc
     1081 ggggtcaccg cttcgcagct ttttcatcgc gagagggaag tgccaggggt agccgcagag
     1141 gagttcatct cctccctgac cgctgaggac ggttttcact ccatcgcccg ccatcgcgcg
     1201 aatcaggcgg aactgcggaa ggggagcgcc ggggatgtgg accgggattt cgagaatccg
     1261 gaaaagctcc ttcagctcgt cgagcttcgc ggggccgtcc ggctcaagca gtactggatc
     1321 gatgcccaga gatttcgcgg cctccagagc gcgctcccgg tcgggtgcgc ccggcggtcc
     1381 gtagacgaag cagcggggag ttcccctctt tgcccatgca gcggctaaag acgacgagtc
     1441 caatccgccg ctcaggcaga gcgcggctgt ttccaaccca tcaccaagag cctcttccag
     1501 caggtcccgc agcaagggga gcgtctcctc ccccagctcg ccggatgcct cgggaagcgg
     1561 ttcccgttcg acgaccgagg cgcttccttc cgagacgatc aggtcatgtc cggggggaac
     1621 ttcgaagact ccctcgaaga atgtcttgcc gccggggaag acgcacgttc ccgtcgcgag
     1681 gaaatcgaca agcgcctcgg aacgccattt cctgggaacg cctgcggcaa aaagcgcttc
     1741 ataggttttt cccgccacaa tctcactttc cagccccgca tagagcaaag gctccacccc
     1801 cgcgcggtcg cgggaaaaac atgccgtgtc cccgggagga agaagactct ttcccggcgc
     1861 ggcacacccg aacaagctcg ctgccgattc catgcacatc ctcagcacct cccttgaatt
     1921 cgacccctcc atcgcggaat ggacggtgaa actgcccggt cctcccgtgt cgtcggcttc
     1981 ggattcattc taaacgttca cactgtgtca aggtcaagaa atattcctcg aaatgctctc
     2041 tttaagaaaa cggatcgaag atctcttcca aaacaaaatg gaagggacgt tttttgaata
     2101 taactctcgc gaaagcagct ttgaaaaggt ttcggagaac tgcttaaaac gtgtcttggg
     2161 cggcaaaaaa gagaaatatg tggctatcgg caaactgttg gggcgggctc attgtaacaa
     2221 aaggatactt attcgacaaa ttccaggagg caccatgaga tagaaggtcc cttcccgaga
     2281 tagcattccg tggtccgccg gtcaggaaag aggctccagg ccaaaattat tcacccgatt
     2341 tccaggaaag acacaggcac tctgcacgtt tttttgatcg accgtcgtgg aaaaaagtga
     2401 ctggccccat cgtgccggaa gggtcacaag gcgctttcat aaacacaacg atcatccgaa
     2461 tcgctcaaat ttggaaagag aggctgacgg tgctgccagg atggaaaaac acgtatcgat
     2521 tgttctgcgc ttttcagaca cgcaagctga ttccatcagg aaaagtgcta tgatatttat
     2581 tattcaaaaa ttgcgtttgc cggtattttg ccggttgatc tacaagcttt tttctgcact
     2641 ggccggggaa cctgctgaag ggacaacagg agcaaaattt ccggtttcca aaggattgtc
     2701 agtttccgaa atgcccaatt ccaaactccg gaggggaacg ctctcatgaa agccgtgcgt
     2761 catgttctgc ggaacttttc tttcaggcag gcggtacctt ttgccgttgc aggtattgtc
     2821 gtgctgttgg ctctttttta catgttccct gtggttcccg acagagatga agacgagttc
     2881 agagcgatgg agatcatcaa ggggttgagg gcggtcaggg ctgccgcgca catgtttttt
     2941 gaaaaaaacc ctgacgtacc ggcgggtcct ttgcatatcg actcgctcaa accgtacacc
     3001 aatgctgaca tctctttgtt tctcattgag agaaatgctc agggagactg gtattgcagt
     3061 tttgggcatg aaatatctgg aaatgtcaga aaaaaactca gagccagagc aagcgagtac
     3121 gaattgctaa aaaatgcgtc tgatcaatat gacggcgggc cggtcgtgtt aatggcggtt
     3181 cggaaatttt aggtatacaa aaacaggaag ggtatttggc acgatggctc gagtaattgt
     3241 ccgtccttag ctttccggtc ttttgaaccg gataacgtgt ctcgttttat tttcttatca
     3301 aaaatcaccg aagaatttta aaggaggctt tcccatgatc atctatcgga acgcggtgcc
     3361 ggacgatctg gccggtgtcg ccgaactgca gaagcggtac cacatttcga ccatccggga
     3421 ggaggacaag gcggacgggt tcgtgaccac tctgttcacc ccggagcagt tcctgtccct
     3481 tatgaaggac gaaaacggac tggcggtggc gtgcgacggc aacaaaatca tttcctatgc
     3541 catggcggcg tcctgggaat actggtccgc atggcccctc ttccggcaca tgatcgccga
     3601 tctgccgaac atcgaatacc tcgggagggt cctttccacg gagaactcat accagtacgg
     3661 gccggtctgc atcgccgggg agtatcgggg acggggagtg ctggagcata ttttcgaatt
     3721 ctcccgccgg cagatggtgt cccgctatcc cgtgatggtc accttcatca acaggatcaa
     3781 cccccggtcc tacgacgccc atacccggaa ggtgggcatg gagctgatca agactttcga
     3841 cttcaacggc aaccactatt acgagctggg gtacgatatg accaggccgg tccccggaac
     3901 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgcagac gacgatcgcg
     4021 cagcatcccc gcagggggag gatctcgctt ttgaggaagg ctaaaaacaa aggcgagatc
     4081 cttcgcttcg ctcaggatga caagcaaggg agagatccct cgcattctct cgggatgaca
     4141 ggcaggggcg aggctatttc tgctcctgct gcagcttctt gaagtcctcc cgcacggggc
     4201 agaagacgtc gatgacgagg cagtcctcca gggcgaagcc tccgtgggcc tcgcctccgg
     4261 cgaagacgat gccctgcccc ggggtgaggg tgaccttatt gtcggcagtg ccgagttcca
     4321 tttttccggc gagcagcagg gtggtctgct cgtgggggtg gtcatggaaa acaagttctg
     4381 ttcccttttt gatctcccac tgggcgaagg tgaagccctc ggagtggatg aatttcccga
     4441 tgaagcccgg tcttccggtg aacttttcga cggtttccat gtcaaatgtc ttcatgtctg
     4501 catgcatcct ttccatttgg tagtgtactc gtcctgcccc tcctggggag aactgggcac
     4561 ccttgacaca atccggtctc gcgagtataa gtaggaatca atttcgtttc caataatatg
     4621 ccatttgcgc gatgaaggga agagtacctt tttccggcgg cggacagaga gggaggaccc
     4681 aggctgagaa tcctcctccg gagcgggaaa gggaagacca cccccgagcg ggcgccgaag
     4741 tcctgaggga gggaaggcgc cggggtccgc cccatacagc gggatcgagc agccgattat
     4801 ttcggaagtg gagtgggacc gcgatgagcc tgcatcgtct ccagcatgtg gacgatgcag
     4861 gctttttttt caggagggag attcatgaga aaaggcattc tgcattcata cgagcgtttt
     4921 tttcccctga cggaccggac tccccgtctt tccctgtgcg aaggcgacac tccccttgtt
     4981 ccgctgcgga atattggccg ggagcttggt gtggaactgt acggcaagta tgacggcctg
     5041 aaccccacgg gatccttcaa ggaccggggg atggtacttg ctgtgtcgaa ggccctggag
     5101 gagggtgcga aggccctcat ctgcgcgtcc acggggaaca cgtccgcctc ggctgcggcc
     5161 tacggggccg ccgcgggaat tccctgcttc gtcctgctgc cggcggggaa ggtggctgtg
     5221 ggaaagctgg cccaggcact gatctacggc gcccgggtca tcgcggtacg gggcaatttc
     5281 gaccgggccc ttgaaatggc cagggaggct tccagggaac ggggaagcct cgccatcgtc
     5341 aactcggtga acccgtaccg gctcttcggc cagaggagcg cttcctggga gatatgcgac
     5401 gaactgggcg gtgcgccgga ctggctggtg ctgcccgtgg gcaacgcggg gaacatcagc
     5461 gcctactgcg c
//
LOCUS       DAOPKU010000157         5466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16117_length_5466_cov_5.064498, whole genome shotgun sequence.
ACCESSION   DAOPKU010000157 DAOPKU010000000
VERSION     DAOPKU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16117_length_5466_cov_5.064498"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1582)
                     /locus_tag="PK849_06220"
     CDS             complement(191..1582)
                     /locus_tag="PK849_06220"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPE65758.1"
                     /translation="MTYRCNKKNCTRCGACSGLGVLSKPLRGLSAPLGSGEETSSRVF
                     SAAIDLGTTTLACYLADVQDGRILAAASARNPQASWGPDVISRIVRASESPGALHELA
                     DVVRNHIGALAANLAGEAGLDVRNCREIAIAGNSVMELLFLGISPEPLGSAPFALPAR
                     SFAPRSVGELEIPFLPPETSVHIFPLIDGFVGGDTTALLYEITEAAALSGSQALTRLV
                     IDFGTNGEIALIHKGRIVACSTAAGPAFEGASVRQGMPALPGAVCGIVSDGGATYCRT
                     VNNAPAIGLCGSGLVDAVALLLREGVLNEDGAMEGRATSSFARRIRGEGSELRCVLCE
                     SKASEVALYQCDVREFQLAKGAVQAGIELLLSHWGVAWEEIGECILAGAFGNAIRHES
                     LLETGVLRGLLRNRIRSAGNGAGMGIVRMLGNGESGRRRVHRIAETAEHLRLESVPEF
                     QSRFLEAMILAPR"
     gene            1818..3413
                     /locus_tag="PK849_06225"
     CDS             1818..3413
                     /locus_tag="PK849_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HPE65759.1"
                     /translation="MRRFMLLFSLCASLLLGLPDAEAVTLTVAAPSEGSRILAPGRDF
                     YAIVSIDRAGKNPEQEPFNVRFELYRDGVLAPLRTVTSTVDDGTGLTPLNAIKTDYPH
                     GWTPGTALDILASPPPDLVYNPALPVSFYDAAIKAVVTRHYAAALIQGGHTKTFDSNY
                     AAIYTRDLEEGAYTLKATAVGAAGAELATVSVPLTFGSVPDKIISRFSPENHMENVTA
                     FAQKNNSHIYNDLFPGYWDASSLPHGGIPGTLFYEIVRRWRPNDLLEYMNGTIRAVVY
                     NVHVSSATQSVELGGLAYAGRLGSNSILWYHYDSGEVSLAYDLGNGQAASKLGTIVPF
                     PDGKRLVLVRAEIRGDGQTEPPTSDYVYTPELADKTVDWNVADGVAVKPKQLLSLFGV
                     VKPIQPALNDVVANDDGTYTINNRIATVRYTLLDGTQEMLTFDAKRVELTRNGINNRP
                     SIYEFRHDIPVPATFNKPWTVRVAAFDIYGTLVPGTEMSFTLAPYSPGGGGGGGGCSV
                     GAAPFALLLLLPLLVLAGKRGRT"
     gene            3447..4172
                     /locus_tag="PK849_06230"
     CDS             3447..4172
                     /locus_tag="PK849_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPE65760.1"
                     /translation="MLLRRERELVVEHCRMLATHKLTKGTGGNISALDREKRLFAISP
                     SGMDYFAMQPEDVVVLDLDGKTVDGERRPSSEMDMHRLLYVDREDINAVVHAHSTYAT
                     TLACLHWSLPALHYLIGYAVSRGPTNTSEASKESCQSLSAHASSARGDGVRCTPYAPF
                     GSTELAEIARDGMKGRYAVLLGNHGLLAAGPNMQYAFNAAEEIEFVCELYWRAKSVGD
                     PVILSEEDMNVALEKFKTYGQRA"
     gene            complement(4293..4820)
                     /locus_tag="PK849_06235"
     CDS             complement(4293..4820)
                     /locus_tag="PK849_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HPE65761.1"
                     /translation="MRSVAEKRESREAVCRKLDEMGIPYEMIEHEAAYTVEDMDRVGF
                     DEKTTVCKNLFVRDQKKKATHWLVVLLKDKRADLDSLAEQLGTKHLSFASADRLQKYL
                     GLARGEVTPLGVLNDAAHEVIVVFDKDLIGRERLGVHPNDNSATLLLSFGDIKRFVEQ
                     NGNTVRYVTLKAETA"
     gene            5089..>5466
                     /locus_tag="PK849_06240"
     CDS             5089..>5466
                     /locus_tag="PK849_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65762.1"
                     /translation="MTEPINFNALFLGPKAENYDFFKQMLVYLMDDHAEWRRFFHPED
                     PLVVTEEEKARQDFARTLQNTREALIELAGNLQVSSNPWFSPRYLGHMNADTLIAANL
                     GYMLTLLYNPNNCAYEGSPATTAL"
BASE COUNT         1043 a         1716 c         1687 g         1020 t
ORIGIN      
        1 ggccgaagat ggagctgctt ccgtatcacg attttggacg tgcgaagtac aggatgctcg
       61 gcatggagtc gttctggacg gagttcgaga ccccttcgaa ggagagggtc gcattcctgg
      121 aagcgctcat ccgctccgaa ggtgtggaac cggtcagcta caggtagggc tgcacgcctc
      181 gcaccccaag tcagcggggc gcgagaatca tggcctccag aaaacgggac tggaactccg
      241 gaactgattc gaggcggaga tgttccgccg tctccgctat ccgatgcacc cgcctgcggc
      301 cgctctcgcc gttaccgagc atgcggacaa tccccatgcc cgcgccgttt cctgcggagc
      361 gtattctgtt gcggagaagg ccgcgcagca cgcccgtctc cagcagcgat tcgtgcctga
      421 tcgcgttgcc gaaagcgccc gccaggatac actcgccgat ctcctcccag gcgacgcccc
      481 agtgcgacag cagcagttcg attcctgcct gaacggcccc cttcgcaagc tgaaattctc
      541 ggacatcgca ctggtacagc gccacttcgc ttgccttcga ttcacagagg acgcagcgca
      601 gctccgaccc ttccccgcgg atacggcggg caaaggacga cgtcgcccgg ccctccatcg
      661 cgccatcctc gttgagcaca ccctcccgca gaagcagcgc gacggcgtcg acgagcccgc
      721 tgccgcagag tccgatggca ggcgcgttat tcaccgtgcg acagtacgtc gcccccccgt
      781 cggagacaat gccacaaaca gcgcccggca gcgcgggcat tccctggcgg acgctcgcgc
      841 cttcgaacgc gggccctgcg gcggtggagc aggcgacgat tctccccttg tggatgagcg
      901 cgatctctcc attcgtcccg aagtcgatga cgagccgtgt cagcgcctga ctccccgaga
      961 gggcggccgc ttccgtgatc tcgtagagca gcgcggtcgt gtcgccgccg acaaatccat
     1021 cgatcagggg gaagatgtgg accgacgtct ccggagggag gaacggaatt tcgagttcgc
     1081 ctacgctccg gggagcgaag cttcgcgccg ggagcgcgaa cggtgcgctt cccagcggtt
     1141 ccggggatat gcccaggaag agcagctcca tgacgctgtt tccggctatg gcaatttcgc
     1201 ggcagttccg gacgtcgagc ccggcctccc cggcgagatt cgccgcgagc gcgccgatgt
     1261 gattccgcac gacgtccgca agctcgtgca acgcaccggg gctctccgag gcgcggacga
     1321 ttctggagat gacgtccggt ccccaagaag cctgaggatt ccgcgccgac gccgcggcga
     1381 gaatgcgacc atcctgaaca tccgcaagat agcaggcgag cgtggtcgtc ccgaggtcga
     1441 tcgccgccga aaacactcgg gacgatgtct cttcaccgga accaagcggc gccgaaagtc
     1501 cgcggagggg cttggagagc actccgagtc cggaacatgc gccgcagcgg gtgcagtttt
     1561 ttttattgca tctgtaggtc attgtctttt ttcctttccc tgcatcaaat aatgaagagc
     1621 ggcgcgtccg cttcgtttac ggccggggct cccggcgctc gcgccctgcg tcggcggcgc
     1681 tccgccatga taccggaaaa gaggcgcgga cggaagttcc cgttgatcag ggtgtgaaag
     1741 acgaggacga agcggccgct tggtggtatg attttctttg atctctctca tcatcttgga
     1801 aagggtgtgt tgttttcatg cgacgcttca tgttgctctt ctccctgtgc gcctcgctgc
     1861 tgctgggtct tcccgatgcg gaggccgtca cgctcacggt ggcagctccg tccgagggtt
     1921 cccgtattct tgctcccgga cgcgacttct acgcgatcgt ctccatcgat cgcgcgggca
     1981 aaaatcccga gcaggagccc ttcaacgtcc gattcgagct gtaccgcgac ggggtgctcg
     2041 ccccgctgcg cacagtgacg agcacggtgg acgacggtac ggggttgacg ccgttaaacg
     2101 ctatcaagac ggactatccg cacggatgga ctccgggaac cgcgctggac atcctcgctt
     2161 cgccgcctcc cgacctcgtc tataatccgg cgctgcctgt ttctttctac gatgcggcga
     2221 tcaaggccgt ggtgacgcgc cactacgccg ccgccctcat ccagggaggc catacgaaga
     2281 ccttcgacag caactacgcg gccatataca ctcgcgatct ggaggaggga gcctacaccc
     2341 tcaaggcgac ggccgtcggc gcagcgggcg cagagttggc caccgtcagc gtacctctga
     2401 ccttcgggag cgtgccggac aagatcatct cccgcttttc tcccgagaac cacatggaaa
     2461 atgtaacggc cttcgcgcaa aagaacaact cgcacatcta caacgatctc ttccccggat
     2521 actgggacgc atcctctctg ccccacggag gcataccggg gacgctcttc tacgagatcg
     2581 tgcggcgctg gcgtccgaac gacctgctgg agtacatgaa cggcacaatc agggccgtcg
     2641 tgtacaacgt ccacgtctct tcagcgaccc aatcggtcga actcggcgga ttggcgtatg
     2701 cgggcaggct aggttccaat tcgatcctct ggtatcacta cgacagcggg gaggtgtcgc
     2761 tcgcctacga tctcgggaac ggtcaggcgg cgtccaaact gggaaccatc gtccccttcc
     2821 cggatggcaa gcgccttgtc ctagtccgcg cggagatacg cggcgacgga cagaccgaac
     2881 ctccgacgtc ggactatgtc tatactccgg agcttgccga caagacggtg gactggaacg
     2941 tggccgacgg cgtcgcggtg aagccgaagc agcttctcag ccttttcggc gtggtgaagc
     3001 ccattcaacc cgcccttaac gacgtggttg ccaacgacga cggaacctac accatcaaca
     3061 acaggatcgc gacagtccgc tacaccctgc tcgacggaac gcaggagatg cttacatttg
     3121 acgccaaacg cgtcgagctg acccgcaacg gaatcaacaa ccgaccgtcc atctacgaat
     3181 ttcggcatga cattcctgtt ccggcgacct tcaacaaacc gtggacggtc cgcgtcgcgg
     3241 cgttcgacat ctacggaacg ctcgtgccgg gtacggagat gagcttcacg ctcgctccct
     3301 actcgcccgg aggcggcggc gggggcggcg gatgctcggt cggcgccgcg ccgttcgcgc
     3361 tgctgctgct tctgccgttg cttgttcttg cgggaaagcg cggaaggacc taggcctacg
     3421 cttgtattgc aatggaggaa cgacgaatgc tgctccggcg tgagcgtgaa cttgttgtgg
     3481 aacactgcag gatgttggcg acccataagc tgaccaaggg gaccggcggc aacatcagcg
     3541 cgctggacag ggagaagagg ctcttcgcga tcagcccgag cgggatggac tatttcgcga
     3601 tgcagccgga ggacgtcgtc gtcctcgacc tcgacgggaa gaccgtcgac ggggagagaa
     3661 ggccgtcgag cgagatggat atgcaccggc tgctctacgt ggacagggag gacatcaacg
     3721 ccgtcgtcca tgcgcactcc acctacgcaa cgacgctcgc ctgcctccac tggagccttc
     3781 cggcgctcca ctacctgatc ggatacgctg taagcagagg accgacgaac acgagcgaag
     3841 cgagtaagga gtcgtgtcag tcgcttagtg cgcatgcatc tagcgctaga ggggacggag
     3901 ttcgctgcac accctacgcg ccgttcgggt cgaccgaact ggcggagatc gcgcgcgacg
     3961 ggatgaaggg gcggtacgcc gtccttctgg gcaatcacgg cctcctcgcc gccgggccga
     4021 acatgcagta cgccttcaac gccgccgagg agatcgaatt cgtctgcgag ctctactggc
     4081 gcgcgaagag cgtgggcgac cccgtcatac tgagcgaaga ggacatgaac gtcgcgctgg
     4141 agaagttcaa gacgtacggc cagcgggcct gaaccgttcg gaaaaatacg catgaaaacc
     4201 gggagccctg aatttcgggc tcccggtttg tttgttccgg agaacggagg aatgcggcgg
     4261 gcaggttccg aagcggggtt tttcgttgcg cgctacgccg tctccgcttt caacgtaacg
     4321 taccgcacag tatttccgtt ctgctcgacg aaccgcttga tgtccccgaa cgacaggagg
     4381 agcgtcgcgc tgttgtcgtt cgggtggacg cccagacgct ccctgcctat aaggtccttg
     4441 tcgaagacga cgatgacctc gtgggctgcg tcgttgagca cgccgagggg cgtgacctcg
     4501 ccccgggcga ggccgaggta tttttggagg cggtcggcgg aggcgaagct gaggtgtttc
     4561 gtccccagct gttccgcaag gctgtccagg tcggcccgct tgtccttgag cagcacgacg
     4621 agccagtggg tcgccttttt cttctggtcg cggacgaaaa ggttcttgca gacggtcgtc
     4681 ttctcgtcga accccacgcg gtccatgtcc tcgacggtgt aggccgcctc gtgctcgatc
     4741 atctcgtagg ggattcccat ctcgtcgagc ttccggcaga ccgcttcccg tgattcacgc
     4801 ttctcggcga cgcttcgcat tgcagcacct tccttgttgg agagatctca ggttgttgct
     4861 tctattcctg ctgaaagaac cgcgtctatg atacccgaaa aggccgtcag gtcaaacgaa
     4921 gaaacgattg tccgaaatga agcggggtgt ggggcgcgcg ctcttgactt tgacacggcg
     4981 ttacggtgta tcatctgggt acggaacgga gacgagcctg cacctggctg gacgaatgag
     5041 ccggcgagta caggcgcgta cctcccccgc ggaaaggatg gcgaatcgat gaccgagcct
     5101 atcaatttca atgcgctgtt tctggggccc aaggccgaaa actacgactt cttcaagcag
     5161 atgctcgtct acctgatgga cgatcatgcg gagtggcggc gctttttcca ccccgaagat
     5221 ccccttgtgg tcacggaaga agaaaaggcg cggcaggact tcgcgaggac gctccagaat
     5281 acgcgagagg cgctcatcga gctggcggga aaccttcagg tcagctccaa cccgtggttt
     5341 tcgccgcgct atctggggca catgaacgcc gacacgctca tcgcggccaa cctggggtac
     5401 atgctcacgc tcctgtacaa cccgaacaac tgcgcctacg agggctcccc ggcgacgacc
     5461 gcgctg
//
LOCUS       DAOPKU010000158         5461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16132_length_5461_cov_4.392527, whole genome shotgun sequence.
ACCESSION   DAOPKU010000158 DAOPKU010000000
VERSION     DAOPKU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16132_length_5461_cov_4.392527"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PK849_06245"
     CDS             complement(<1..820)
                     /locus_tag="PK849_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE65763.1"
                     /translation="MKLWNTNPAHGIRLTGGKGSALYDDQGRTYIDMFSGTWCNVLGY
                     GHPRLRRAVEEQAQGLFQTGAAFGTRQVEEGLRQLASILPPELERAVFLNSGSEAVEL
                     ALKMAHAATGKTGVVVAEKGYYGGTVYALSLSEAGRTASYLPKAGNVLRVAAPHCVRC
                     DLSAECGDGEFPCMNVLEDLSPEEWPAAILYEPLLGGGILVPPIGYGARLKEFARSLG
                     ALFISEEVTTGMGRTGKWFGFQHENIVPDILVVGKAIGGGLPVSAVVTTKEVEER"
     gene            complement(817..2310)
                     /locus_tag="PK849_06250"
     CDS             complement(817..2310)
                     /locus_tag="PK849_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPE65764.1"
                     /translation="MLLFELDERAPQSKSWQIVKRLLEMMKNGALTPGDRLPSTRKLA
                     ELLRVHRSTVALAYQTLWSLGYVELRRGTAPVVRCRVRVAEPGDREPLSAIDWSTVAS
                     PASEEVYRLFLARNVRREHTPDLQCISFEFIKMDPRLFPAERFRSSLGRVFRDEGLSL
                     LGYGDAQGYEPLREAIAVRMRTHGISVTREEIVITNGAQHALDLVLRMTARPERTVAV
                     ESPCYGDILPLLKQYGLNIAEIPMNCEGMDLDVLEDRCRKSPLHLIYTMPNFQNPTGI
                     TTDQAHRERLLSICSRYEVPLLEDAFEEEMKYSGRAVLPVKSMDSGHRVIYCGTFSKV
                     LFPGIRVGWAAAHPECIRRLTALRRYGELAPPGLLQAALARFCSEGWYEKHISMMHRV
                     YRKRMQTALAALERFVSPEWASWTKPAGGYLIWLSMAPHPPVDWEKVFASEGVAVASG
                     RSFFSSDPAGTFFRLSISSLNEAEIEEGIRRLGKAFQKVCYRKDIAK"
     gene            complement(2383..3054)
                     /locus_tag="PK849_06255"
     CDS             complement(2383..3054)
                     /locus_tag="PK849_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65765.1"
                     /translation="MLKVRSIFNCLEYAGALPALPIRRRLFLFFAVFCFAAFPASASA
                     RTFSPQADGRNAETVPFSSIREAYRQKAFDLWKRAADLQIGGRYEEALEKYREGLGYH
                     EDPRVREHVWKLEETLAARRGSENARKQAARRAAAELWKEAAALQQAGWFEDALQKYK
                     EGLELSDDQRVREHVKKLEEYIERLRHRGLLPVLVFMRERTGDALFKTPSSGVFFVKV
                     VRQKN"
     gene            complement(3174..3719)
                     /locus_tag="PK849_06260"
     CDS             complement(3174..3719)
                     /locus_tag="PK849_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HPE65766.1"
                     /translation="MKQSRYALRDMVVLSLLIGLNVVLTRIASIRIPIGGVESLRIGF
                     GAVPVIFAGLFFGPRAGAVVGALGDVAGFIMSPAGGAFMPHFTLTAALTGAIPPLALR
                     LFRHREHVAEHSFIELLVAIAAGQLITSVLMVPYFLNMLFGVPFTATLIVRLQTQLLQ
                     IPLFVMCIKLLARRLSFIVAG"
     regulatory      complement(3787..3886)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(4050..5198)
                     /locus_tag="PK849_06265"
     CDS             complement(4050..5198)
                     /locus_tag="PK849_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65767.1"
                     /translation="MKMFKILMIVVVILGCASVSVGAENEDDSIFAKMWSKAMPKLEQ
                     ALELFERGETLPESKIFGADKQSNNAKYEKLLNEVFDILLDSEFHASRGEYRSLEERI
                     EKSEQKIAELRKERIGAPEESWIPFRQTRSKIDEEVDELKEDIARFRKEKEAIRSSMV
                     AALSAMGVSVDGAKLDILLASVSGDDVIALLLAADSIKKINLQIVDLLRKGEGNIHMA
                     RKYTGVHMVLVDIYLHAHHDVLEKIASEYSPRLDSITSSAQLLKKQAEQMLAATPRGG
                     SQREVLETNVKANERTVQTASLYRDYLRTQQNKLRISAEKLTSDLSVARNTYQTVKTG
                     GDLISLINISMRTLESVFGFVLPDLSTVYDQEVLKEFERITGQIRKGS"
BASE COUNT         1259 a         1638 c         1389 g         1175 t
ORIGIN      
        1 cccgctcttc cacctctttc gtggtgacca ccgccgagac gggcaggccg ccgcctatgg
       61 ccttaccgac gaccaggata tcaggcacga tattttcatg ctggaagccg aaccacttgc
      121 cagtccggcc cattccggtg gtcacctcct cggagatgaa gagcgccccg agagaacggg
      181 cgaactcttt gagccgtgct ccgtagccga tgggcggcac aaggatgccg cctccgagga
      241 gcggttcgta gagaatcgcc gcaggccatt cttcaggcga cagatcctca agaacattca
      301 tacagggaaa ctcgccatcg ccgcactccg ccgagagatc gcaccggacg cagtgcggcg
      361 ccgcgacgcg gaggacgttt cccgcttttg gcagatatga agcggtccgc ccagcttcgg
      421 agagcgagag cgcgtaaact gtgcctccgt aatacccctt ctccgcgacg acgactccag
      481 tctttcctgt cgcggcgtgc gccattttga gggcaagctc cacggcttcg cttccggaat
      541 taagaaaaac cgccctttcg agttccggag gaagaatcga agcaagctgc cggaggcctt
      601 cctctacctg tcgcgtgccg aaagcggccc ctgtctggaa aagcccctgc gcctgctcct
      661 cgactgcgcg acgcaagcgg gggtggccat acccgagtac gttgcaccac gttcccgaga
      721 acatatcgat atacgttctc ccctgatcgt cgtagagcgc actccctttc cccccggtaa
      781 gacggatgcc gtgcgcgggg ttggtattcc aaagcttcac ttcgcaatat cctttctgta
      841 gcagactttc tggaacgctt tcccgagcct gcgtatcccc tcttctatct cggcctcgtt
      901 cagcgaagaa atggagaggc ggaagaacgt accggccgga tccgacgaaa aaaacgaccg
      961 tcccgaagcg acggcaaccc cctcggacgc aaaaaccttc tcccagtcga cgggaggatg
     1021 gggagccatc gacagccata taaggtaccc tcccgcgggt tttgtccaac tcgcccactc
     1081 cggggagacg aaacgctcaa gggccgccag cgcagtctgc attcgctttc gataaaccct
     1141 gtgcatcatc gagatatgct tctcatacca tccttcggaa cagaatcggg caagtgcagc
     1201 ctgtaacagc cccggaggcg caagttcacc gtaccggcga agcgctgtca agcgccggat
     1261 acactccgga tgcgccgctg cccaccccac gcgtattccc gggaaaagaa ccttcgaaaa
     1321 cgtgccgcag tagatcactc tgtgtccgct gtccattgat ttcacgggaa ggaccgctct
     1381 acccgagtac ttcatctcct cttcgaacgc atcttcaaga agcgggactt cgtagcgcga
     1441 acagatggag agcaggcgct ccctgtgcgc ctggtccgta gtgatccctg tggggttttg
     1501 aaagttcggc attgtgtaga tcaggtgaag aggggatttt ctgcagcgat cctcaagtac
     1561 gtcaaggtcc atgccttcgc agttcatcgg aatttcggcg atgttcagcc catactgttt
     1621 caggagaggg agaatatcgc cgtagcaggg ggactccacc gcgaccgttc tttctggccg
     1681 tgcagtcatt cgaagtacaa ggtcaagcgc atgctgcgcc ccgttcgtta ttacaatctc
     1741 ttcacgtgtc acggagatac cgtgcgttcg catccgaacg gcgatcgcct ctcgaagcgg
     1801 ttcatacccc tgcgcatccc catagccaag aagagaaagt ccctcatccc tgaagactct
     1861 tcccagagac gagcggaacc gttctgcggg gaaaaggcgc gggtccatct tgatgaactc
     1921 aaaggagatg cactgcaagt caggagtgtg ctcacggcga acattccggg caagaaaaag
     1981 gcggtacacc tcttcggagg cgggagatgc gacagtcgac cagtcgatcg cgctcagagg
     2041 ttccctatcg cccggctcag caacccgaac acggcaacga accaccggag ccgttcctct
     2101 ccggagttcg acatacccga gagaccagag cgtctggtag gctaaggcga ctgtggaacg
     2161 gtgcacccga agcagctccg ccagtttcct cgttgaaggg agtctgtcac cgggggtcaa
     2221 cgcaccgttt ttcatcattt caagaagacg cttcacgatc tgccagcttt ttgactgcgg
     2281 cgcgcgctca tccaactcaa aaaggagcat gggctccctc ctttccggag atctacgcgc
     2341 actacactac accgcatgtg gccccttcac aactgccact ttttagtttt tttgacgtac
     2401 cacttttaca aaaaaaacgc ccgatgaggg cgttttaaaa agagcatctc cagttcgttc
     2461 tctcatgaaa accagaaccg gaagcagtcc tcgatgccga aggcgttcga tgtactcttc
     2521 aagttttttc acatgctcgc gcacccgctg gtcgtcggat aattccagcc cctccttgta
     2581 cttttgaagc gcatcctcaa accaccccgc ctgctgaagc gcagcggcct ccttccagag
     2641 ctccgccgcc gccctgcgcg cggcttgttt tctcgcgttc tcactgcctc ttctcgccgc
     2701 aagcgtctcc tcaagcttcc agacgtgctc tctcacccgt ggatcttcat ggtatccaag
     2761 cccttcccga tacttctcaa gcgcttcttc gtaccgtccg ccgatctgca agtcggcggc
     2821 ccgcttccag agatcaaacg ccttctgacg gtaggcctcg cgaatcgagg agaagggaac
     2881 ggtttccgcg ttccgcccgt cagcctgcgg agaaaaggtc ctggctgacg cagacgccgg
     2941 gaaggccgcg aaacaaaaca ctgcaaaaaa gaggaacaac cttcttcgga ttggcagcgc
     3001 cggcagagcg ccggcatact ccaaacaatt aaagatgctg cgcactttca acatgctttc
     3061 tcccctcctc ctccggtcga cgacgtcgca ttgaggaacc gacgaaaaaa cgcgatcaaa
     3121 aaaagacgct cttctcggga cgtttcagaa gggcgtcttt actttttcat tggttacccg
     3181 gcgacgatga acgagagacg gcgagcgagc agtttgatgc acatgacgaa gagcgggatc
     3241 tggagcagct gcgtctggag gcgcacaatc agcgtcgctg tgaacggaac gccgaagagc
     3301 atattcagaa aatacggaac cataagcacc gaggtgataa gctgtcccgc cgcgatggca
     3361 accagcagct caatgaagga gtgttcggcg acgtgttccc gatgccggaa gagcctgagc
     3421 gccagcggag ggatagcccc cgtgagcgcc gccgttagtg tgaagtgggg catgaaggcg
     3481 ccgcctgcgg ggctcatgat gaatccggcg acatcgccga gcgcgccgac gactgcccct
     3541 gcccgcggac cgaagaagag gcccgcgaag atcaccggaa cagcgccaaa accaatcctg
     3601 agcgattcga cgcccccgat ggggatacga atgctcgcaa tacgagtcaa caccacattc
     3661 aaaccgatca aaagggaaag gacaaccata tcgcggagag cataccgaga ctgtttcatg
     3721 aaaaaaacct ccttatgaca taccggcaca accgccacat caaaagaggt ctgaaccaat
     3781 tgatgtgaca gcgaacgcga cttccccacc gcaaatcacg aatgattttt cgtcccgggg
     3841 caacgtccca tcccagggca cttcacgcgg gaaaacctac tctgcctacc tttattcgta
     3901 gcattatacc atctttcaag aaaaaacaag cgcacatcgt gctgcatatt gtcaagaatc
     3961 cgagggctgc agaacactcg cgaggcgtac aggtgaaaaa gaaacgccgc ttccagaact
     4021 tgttacatcc gggaaacggc gcttcaggct caactccctt tgcgtatctg ccctgtaata
     4081 cgttcgaact ccttgagcac ttcctggtca tacaccgtgg aaagatccgg aagcacaaaa
     4141 ccgaagacgc tttcgagcgt ccgcatggag atgttaatca gggatatcag atcgccgccg
     4201 gtttttactg tctggtatgt attccgcgca accgaaagat cactcgtgag tttctctgcc
     4261 gagatacgaa gcttattctg ctgcgttcga aggtaatccc tgtacaggga cgccgtctgg
     4321 accgttcgtt cattcgcctt tacgttcgtc tccaacactt cgcgctgact gccgcccctt
     4381 ggtgttgctg ccagcatttg ctccgcttgt ttttttaaga gctgcgcaga acttgtgatc
     4441 gaatcaagcc gcggcgagta ctccgaggcg atcttctcaa gaacgtcgtg atgcgcatga
     4501 agatagatat ccacaagaac catgtgaacg ccggtatact ttctggccat gtggatattc
     4561 ccttctccct tgcgcaggag atctactatc tggagattaa tcttttttat actgtccgcg
     4621 gcaagcagaa gagcaatgac atcgtcgccg ctcacgcttg caagcaggat atccaatttg
     4681 gccccgtcga cgctaactcc catggcgctt aacgcagcca ccattgaaga acgaatcgcc
     4741 tctttttctt tcctgaagcg agcaatatcc tccttgagct catcaacttc ctcgtcaatt
     4801 ttggaacgtg tctgcctgaa gggaatccag ctctcctccg gggcgccgat gcgttccttt
     4861 cgcagctccg caatcttctg ctccgatttc tctattctct cttccaaaga acggtactcg
     4921 cctctggagg catggaactc cgaatcgaga aggatgtcga agacctcatt caagagcttc
     4981 tcatactttg cgttattgga ctgtttatcg gcgccgaaga tctttgattc aggcagagtt
     5041 tccccgcgct cgaagagctc gagagcctgt tcgagtttcg gcatagcctt actccacatt
     5101 ttggcaaaga tggaatcgtc ttcgttttcc gccccgacgc ttaccgaagc gcaccccagt
     5161 ataactacta caatcatgag tatcttgaac atcttcatag ttatcacctc gtgagagtaa
     5221 tctatcacaa aaaagctttt ctttgttcgt cattcctgcg taccgcaaaa aaagtttcgt
     5281 gcgacctcga cgatcaggcg ttttccataa gctcgcgctt caaatgatat actcttttaa
     5341 gcaaaacgtc attccggagg tgaagctttt gaaacgggta agcatactga atcatgtcat
     5401 cggcccggtt atgcgcgggc cttcaagctc ccacacggca ggtccatggc gaatcggaag
     5461 a
//
LOCUS       DAOPKU010000159         5461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16133_length_5461_cov_4.165187, whole genome shotgun sequence.
ACCESSION   DAOPKU010000159 DAOPKU010000000
VERSION     DAOPKU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16133_length_5461_cov_4.165187"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PK849_06270"
     CDS             <1..538
                     /locus_tag="PK849_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HPE65768.1"
                     /translation="EINGLDERPDDPDPFNIYADPEAAHVVFTCGRPIVMMGLDVTRK
                     ANATAEMVAKVRSLGNPQAILFAEMMEFFAKSQKEVFGWDAPPLHDPTCIAWLIDPTC
                     FTTKPMRVEIELRGSLTYGRTSCDYFGLEHQEGPGKELYEGGGALARPTGPNAEVAVD
                     VDFEKFWKIIYDCFALYK"
     gene            653..1948
                     /locus_tag="PK849_06275"
     CDS             653..1948
                     /locus_tag="PK849_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HPE65769.1"
                     /translation="MDTLNKTLRLHRDEYVARLLDLASLDTAVIGHGIEGGGEERGQL
                     WMEALLREMGAETVREPLTEEIIQDGLARFGEGNPGHVYSGDGFSRWNLIGHFKGGEG
                     RSLLFDGHIDTMPSGPRELWSFDPLDAQMRDGKIWGLGTCDMKGGLLASVLGVKLLQD
                     AGIPLPGDVHILSVVDEEGGGNGSLAAMLSGHRADAAVVCEPSNGSVTVAHMGFVFFK
                     VEVRGVALHSGSKWKGVNAIEKAVLLMGALDDLEHEWLMRYKHPLLPPPTLNIGVIEG
                     GTAGSTVPDRCVFKLCLHYHPETMSHDSVVKEVTDRLLLRAAGDPWLRDNPPEISIYQ
                     AGGAFEMERGHPFVLAVEESIASVRKEAPPVVGSPAGNDARLLRNIGTMPVVITGPGR
                     QEQCHSPDEYIEANEYLEYIEIYARLILDWCGRHKEERS"
     gene            1945..2691
                     /locus_tag="PK849_06280"
     CDS             1945..2691
                     /locus_tag="PK849_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HPE65770.1"
                     /translation="MNKRVLIAGESWVKHISHIKGFDIFTSCEYETGITWLKEALEKH
                     GFEVVHLPGHEVADGFPFTAKELAAYGAVALSDIGANSLLLSGDTFNRSKVMPNRLEV
                     VREYVEQGGGFAMIGGYLSFAGIDGKARYADTAIDEILPVKIANFDDRVEKPEGITPV
                     TTEEGHPMMQGIGAWPHFLGYNRTTLRPEGRLLASAGKDPFVAVRLVGKGRTAAFASD
                     CAPHWGPQEFVEWANYPLFWSRFFGWLCGE"
     gene            2758..2895
                     /locus_tag="PK849_06285"
     CDS             2758..2895
                     /locus_tag="PK849_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65771.1"
                     /translation="MKDVEKTPKMIEKQQRLLYHAFSEAAAEGDVEFASEAQSEILAD
                     E"
     gene            3318..3680
                     /locus_tag="PK849_06290"
     CDS             3318..3680
                     /locus_tag="PK849_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65772.1"
                     /translation="MIATREIRPIKTTDDYEEALREIDGLMDAEPDTPEGDRLDVLTT
                     LVEVYEARTFPMPPVDPVEAVRFAMERKGLTPVDLVPCIGRLNRVYEVLNRKRGLSLR
                     MVRNLHKELGIPLEFLIR"
     gene            complement(3776..5446)
                     /locus_tag="PK849_06295"
     CDS             complement(3776..5446)
                     /locus_tag="PK849_06295"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPE65773.1"
                     /translation="MRTTLLSVNRRYLRFLVLFLALFFVSASEGAVLYVNTGAAGAND
                     GSSWTNAFRSLQDALTASVDGDEIWVAAGVYRPTTGIDRSISFVMKNGVTLYGGFAGT
                     ETLQKQRKWRLNETVLSGNIGVPGSTTDNSYHVVVADGTNSSAVLDGFTITGGNANGA
                     TDEFKRGGGMHNDGGSPTVRNCRFVSNHADKYGGGIYNYNGSPAITNCIISENSSGEY
                     GGGMHIWWNASTPVLTNCTFTSNTAYWGGGACTYLSGTSKFIECVFEYNSASGYGGGL
                     FDSDGKPELTDSVFTGNSAAIEGGGVYNSGGSGTKTITGCTFSHNSAGEHGGGIYNKS
                     VFDKLSVTNSTFFGNTAPTGGGMYNWSASSPIVTNCTFSANEATAGGGMFNTNWSNPE
                     LMNCTFAGNRADFGGDAVFNQVGSAPVATNSIFWGFVAPFGQIQNENTATFAVTYSVT
                     DQPGTGNITDDPLLGALADNGGPTRTHALLAGSGAVNAGTLAGAPATDQRGIPRPQGA
                     GVDIGAYEQGGATPVPVPGPSGSGGGCTVGFSPWALLLLLPLSFLTRR"
BASE COUNT         1054 a         1594 c         1749 g         1064 t
ORIGIN      
        1 cgagatcaac ggcctcgacg agcgcccgga cgatccggac ccgttcaaca tctacgccga
       61 ccccgaggcg gcgcacgtcg tcttcacctg cggacgcccg atcgtcatga tggggctcga
      121 cgtcacgcgc aaggcgaacg cgaccgccga aatggtggcg aaggtccgct cgctcggcaa
      181 tccgcaggcg attctcttcg ccgagatgat ggagttcttc gccaagtcgc agaaggaggt
      241 cttcggctgg gacgcgcccc ctctgcacga tcccacctgc atcgcgtggc tgatcgaccc
      301 gacgtgcttc acgacgaagc cgatgcgcgt ggagatcgag cttcgcggct ccctgaccta
      361 cgggcggacg tcgtgcgact acttcggcct tgaacatcag gaggggccgg ggaaggagct
      421 gtacgaggga ggcggcgcgc tcgcgcggcc aaccggcccc aacgcggagg tcgccgtgga
      481 cgttgacttc gagaagttct ggaagatcat ctacgactgc ttcgcgcttt ataagtagac
      541 gaccggcgaa catgagcgaa gcgaataaga agccgcgtcg gtcactctcg tgcccgcagc
      601 aaagcggagg gtatagagcg catgtttctg gcgctcggag acgatcaaag tgatggatac
      661 tctaaataag actcttcgcc tgcatcggga cgaatatgtc gcgcgtttac tggatctggc
      721 ctcgctggac accgcggtga tcggccacgg cattgagggc ggcggcgagg agcggggcca
      781 gctctggatg gaggccctgc tgcgggagat gggcgccgag actgtccgcg aaccgctgac
      841 cgaggagatc atccaggacg gtctcgcccg cttcggcgag gggaatcccg gccacgtcta
      901 ctcgggcgac ggcttctccc ggtggaacct gatcgggcat ttcaagggcg gggaggggcg
      961 gtcgcttctc ttcgacggac atatcgacac catgccgtcc ggcccgaggg agctgtggag
     1021 cttcgaccct ctcgatgcgc agatgcgcga cgggaagata tgggggctcg ggacgtgcga
     1081 tatgaagggg gggctcctag cctccgtgct cggggtgaag ctgctgcagg acgcggggat
     1141 tccgctcccc ggcgacgtgc atatcctctc cgtggtggac gaggagggcg gggggaacgg
     1201 ctctctcgcc gcgatgctct ccggccacag ggcggacgcg gcggtggtct gcgagccgtc
     1261 gaacggctcg gtcaccgtgg cgcacatggg gttcgtcttc ttcaaggtgg aggtgcgcgg
     1321 cgtcgcgctg cactcgggga gcaagtggaa gggcgtcaac gccatcgaaa aggctgtact
     1381 gctgatgggt gcgctcgacg acctcgaaca cgaatggctg atgcgctaca agcaccccct
     1441 tctcccgcct ccgacgctca acatcggcgt catcgagggg ggaacggcgg gttccaccgt
     1501 gcccgaccgc tgcgtcttta agctctgcct ccactatcac ccggagacga tgagccacga
     1561 ctccgtggtc aaggaggtga cggatcgctt gctgctccgc gctgcgggcg acccgtggct
     1621 gcgggacaac ccgcctgaga tctccatcta ccaggcgggg ggcgcgttcg agatggagcg
     1681 ggggcaccca ttcgtgctcg ccgtcgagga gagcatcgcg tccgtgcgca aagaggctcc
     1741 gcccgtggtc ggctccccgg cggggaacga cgcccggctg ctgcgaaaca tcggcacgat
     1801 gcccgtggtg atcacgggac cggggcggca ggagcagtgc cactctccgg acgagtatat
     1861 cgaagcgaac gaatatctgg agtacatcga aatctacgcc cgattgatcc tcgactggtg
     1921 cgggcgtcac aaggaggagc gttcatgaac aaacgcgtgc tgatcgcggg agagtcgtgg
     1981 gtgaagcaca tctcccacat caaggggttc gacatcttca cgagctgcga gtatgagacg
     2041 ggcatcacat ggctgaagga agcgctggag aagcacggct tcgaggtcgt ccatctgccg
     2101 gggcacgaag tcgcggacgg ctttcccttc acggcgaagg agcttgccgc gtacggtgcg
     2161 gtcgccctct ccgacatagg ggccaactcg ctgctgctct cgggcgacac gttcaacaga
     2221 agcaaggtga tgccgaacag gctggaggtc gtccgggagt acgtcgaaca ggggggcggc
     2281 ttcgccatga tcggcggcta cctctccttc gcggggatcg acggcaaggc gcgctatgcc
     2341 gacacggcta tcgacgagat actgcccgtc aagatcgcga atttcgacga cagggtggaa
     2401 aagcccgagg gaatcacccc cgtcaccacg gaggaggggc acccgatgat gcaggggatc
     2461 ggcgcctggc cgcactttct ggggtacaac cggacgacgc tccgccccga aggccgcctg
     2521 ctggcctcgg ccggcaagga ccccttcgtg gcggtccgtc tcgtcggcaa ggggcgcacg
     2581 gccgccttcg cctccgactg cgccccgcac tgggggccgc aggagttcgt cgagtgggcg
     2641 aactacccgc tcttctggag ccgcttcttc ggctggctct gcggcgagta gggatggatg
     2701 gaaagagggc ggcgaaagcc gccctctcgt cgtaaaaaag agaagagggc ggagtctttg
     2761 aaagatgtcg agaaaacacc caagatgatc gagaaacaac agcgtcttct gtaccacgct
     2821 ttcagcgaag ctgcggccga gggggatgtc gaattcgctt ccgaggcgca gagcgagatt
     2881 cttgccgatg aatagtccac aacgggagaa agatatgcct cgtaggggga ttttcgtcga
     2941 actggtgaga acaggccaag agaatcttct tgcctaaatc ccgaaatggg actagaatat
     3001 ctctatgaga atcattgcgc tttcaacatt gaaggctttc tatgaacaaa gcgaatttca
     3061 tgacgccgga acgccattgg ctcaatggta ctcttttgtt aaaagcgtac gctgggcatc
     3121 tccggcggat gtgaagagag actttggaag cgcaagtatc ttgaaatccg gtcgggcagt
     3181 tttcaacata gcaggagata aataccgttt agtggtaaag ataaactatg gatacggggt
     3241 agtctacatt cgcttcatcg ggacgcatga ggcctacgac cgtatcgacg cttcagaagt
     3301 atagggggtg tggagaaatg atcgcgacgc gggagattcg accgattaaa acaaccgatg
     3361 attatgagga ggcgcttcgc gagatcgacg gccttatgga tgcggagccc gatacgccgg
     3421 agggggaccg gctcgatgtg ctgacgactc ttgtcgaagt ctacgaggcg cgcactttcc
     3481 ctatgccccc ggtcgatcct gtggaagcgg tccgatttgc gatggagcgg aaggggttga
     3541 ctcccgtcga tcttgtcccc tgcatcggga ggctgaacag agtctacgaa gtgctgaacc
     3601 ggaaacgggg gctttccttg cgtatggtgc gcaatttgca caaagagctg gggattcctt
     3661 tggagtttct gatccgttga aatgagcgct gcgaatcttt cttctttttg gtcaaagaac
     3721 ggaaccatac acaatagggc ggcttcggcc gccctattgt gcgttcagca aaccgctacc
     3781 ggcgcgtcag gaacgacaat ggcaggagaa gcagcagcgc ccacggcgag aatccgacgg
     3841 tgcacccgcc cccgcttccg gatgggccgg gaacgggaac cggcgttgcg ccgccctgtt
     3901 cataggcgcc gatgtccacc cccgcgccct gggggcgtgg gatcccgcgc tggtcggtcg
     3961 cgggggcgcc cgccaacgtt ccggcgttta cagcgccact gccggcgagg agcgcgtgcg
     4021 tcctcgtggg gcctccgttg tccgcaagag ccccgagcag aggatcgtcc gtgatgttcc
     4081 ccgttcctgg ctggtcggtg acggagtagg taacggcaaa tgttgcggtg ttttcgtttt
     4141 ggatctgtcc gaatggggcc acaaaccccc agaagatgga gttcgtcgcc acgggagcgc
     4201 ttcctacctg gttgaacacg gcatcccctc caaaatcggc cctgttcccc gcaaaggtgc
     4261 agttcatcag ctccggattg ctccagttcg tgttgaacat tcctccgcct gcggtcgcct
     4321 cgttcgccga gaacgtgcag ttggtgacga tggggctgct ggcggaccag ttgtacatac
     4381 ctccgcccgt aggtgcggta ttcccgaaga aggtggaatt cgtcacggaa agtttgtcga
     4441 atacgctttt gttgtatatc ccgcctccgt gctctcccgc gctgttatgc gagaaggtgc
     4501 acccggtgat cgttttcgtc ccgctgccgc cgctgttgta tacgccgcct ccttcgattg
     4561 cggcgctgtt tccggtgaag acggaatccg tcaattcggg cttcccgtcg ctgtcgaata
     4621 gcccgccgcc gtaaccggag gcggagttgt attcaaatac gcactctatg aacttcgacg
     4681 ttccgctgag ataggtgcac gcgccgccac cccagtaagc ggtgttggag gtgaatgtgc
     4741 agtttgtcag cacgggggtg gaggcattcc accagatgtg catcccgccg ccgtactctc
     4801 cggagctgtt ctccgaaatg atgcagttcg tgatcgcggg gcttccgttg tagttgtaga
     4861 ttccgccgcc gtacttgtcc gcgtggttcg agacgaaacg gcaattccgc accgtcggac
     4921 tccccccgtc gttatgcatc ccgccgcccc gtttgaactc gtccgttgcc ccgttcgcgt
     4981 tgcctccggt gatggtgaat ccgtcgagga cggcgctgct gttcgttcca tcggcgacca
     5041 ccacgtgata gctgttgtcc gtggtcgatc cgggaacgcc gatgttcccc gacagcacgg
     5101 tttcattgag tctccatttc cgttgtttct gcaacgtctc tgttccggca aagcctccat
     5161 agagcgtcac gccgttcttc atcacgaagg atattgaacg gtcgattcca gtcgtcggcc
     5221 tgtacacacc ggccgctacc cagatctcgt ccccgtcgac cgatgcggtc agcgcatcct
     5281 gcaggcttct gaaggcattg gtccacgacg agccgtcgtt tgctcctgcg gcgcctgtgt
     5341 tgacataaag gacagccccc tccgacgcag atacgaaaaa gagcgcgaga aacagcacca
     5401 gaaagcgcaa ataccgtctg tttactgaaa gcagagtagt acgcatacga tcatctcctt
     5461 t
//
LOCUS       DAOPKU010000160         5396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16418_length_5396_cov_4.722898, whole genome shotgun sequence.
ACCESSION   DAOPKU010000160 DAOPKU010000000
VERSION     DAOPKU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16418_length_5396_cov_4.722898"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PK849_06300"
     CDS             <1..1360
                     /locus_tag="PK849_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPE65774.1"
                     /translation="VAKVDGQHQSSKCIHGGAAFIWPVIQAYHFLDLTPLSIEVNLRN
                     ALSKQNIRVDVPSRFTVGISTEPGIMQNAAERLLGLQLAEIQELSKDIIFGQLRLVIA
                     TMDIEEINTDRDKFLANVTANVEAELKKIGLRLINVNVTDINDESGYIQALGKEAAAK
                     AVNDAKKSVAEQDRDGSIGEANAQRDQRIQVAQANAAAVEGENLSKITIAQSEAERRE
                     KQAESNRLAEAAEKVQAAKALEESYKAEEEAELARARREKATQEADVLVHAGIEKERI
                     QIRADADAEEMRRTAAGEADAIFAKMSAEARGLREILVKQAEGFERLVKAAGGDTDKA
                     IMMMIADKLPELVKTQVEAIKGIKIDKVTVWDSAGGASDKTSTANFLSGLMKSIPPLQ
                     DMFKMAGMELPAYMKGGQKNEAPWPVPHEAGPERWMADAGEPVETDPHREMPPEVETE
                     HA"
     gene            1441..2442
                     /locus_tag="PK849_06305"
     CDS             1441..2442
                     /locus_tag="PK849_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HPE65775.1"
                     /translation="MKRKVLFVFICIALSLFFVEVFGAWGVGGAFAEELTLIEQQKLA
                     RTLFNKIAAKDEQDVQGIEPLFLEVIEKAPRVRRAEEAYWMLSNMYMQAFDPPRNKDA
                     VALLEQYLTRYPRSRVLDDKFSMFTTPGLPLVKGRLLSLYEQEEMWEKAAALYDEVIP
                     EPKTAGEALLSHFIGYGRAMEQTGHKEKAIAAYTVFLERSGNTGDFLEEIVKDRLKEL
                     GVEVSSDTSVADGTVPEDPGNLESFRGRNGEVFRFRVRGSALGSLWGDGVYTDDSSLA
                     TAAVHAGVLRQEEEGVVAVRILPGRKEYAGAERNGVVSESYGEWHGSFEFVEAVGDS"
     gene            complement(2576..3073)
                     /locus_tag="PK849_06310"
     CDS             complement(2576..3073)
                     /locus_tag="PK849_06310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPE65776.1"
                     /translation="MSWRCVPLSPAHDRKTFVCGVAELDEYFRNRAGQDQKRGFASVY
                     VAESETGKKDAVCGYYTLSAAGVLLVNLPEALRVKMPRYPSVPAVRLGRLAVSLDCRG
                     RGLGEYLLIDAIRRSLSASVGWTLFVVDAKDENARRFYLHFGFASLPKEERTLYLRRK
                     DIEGL"
     gene            complement(3070..3357)
                     /locus_tag="PK849_06315"
     CDS             complement(3070..3357)
                     /locus_tag="PK849_06315"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HPE65777.1"
                     /translation="MEKTAVKESRIEARIPSDLKKHLARAAALQGRTLSDFVVSAAAE
                     AARRTIEEDALLRLCEEDSRALALDLCAPEREPSPRLRVAAARYDAAKQPE"
     gene            complement(3491..4003)
                     /gene="tpx"
                     /locus_tag="PK849_06320"
     CDS             complement(3491..4003)
                     /gene="tpx"
                     /locus_tag="PK849_06320"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPE65778.1"
                     /translation="MERRGITTMKGNPMTLVGPGLQPGDKAPDFLVLDQTMAPKTLKD
                     YAGKFKVISVTPSLDTPVCDLQIHWFNEDASKQPQDVAVLNISMDLPFAIKRFCATGG
                     IENAVALSDHRDASFGTNWGVLMKELRLLARSIFIVDKEDVVQYVQIVPEQTAEPDYE
                     AALAALKELM"
     gene            complement(4120..5241)
                     /locus_tag="PK849_06325"
     CDS             complement(4120..5241)
                     /locus_tag="PK849_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65779.1"
                     /translation="MIDKNRFGILAVAFVWFTTHFGGGFASGVQVVQYFVSYGWYALF
                     LPVLSIAIVTAVLYYAWTFAAIYCTFDYREWGNKYFKPFEALFSNAYEVMYILILLIA
                     SAVAFATGGTVMQQILGTPYVMNTVIVAVCIFFLTIFGADLVRKAASTMAVIIIAGML
                     LIYVGNLIVNFPKLLAVLKEAPAPASFLDALWASLKYAGFQACAMGAYIAVADALKSR
                     EDAKKASIWGFIVNAGVLWLATAGILLHYPAILTEKVPVLFVAAHGGGGAIGTAIVSL
                     LIFLAVISTGVSLIFGGARRIVGLWGKMKGSTVAGDRKVNIAASLIYVLITWGIALFG
                     LIPLIAKGYGYTGVLSFPLLIVPVLLIGVMRSGKWGKEL"
BASE COUNT         1114 a         1567 c         1756 g          959 t
ORIGIN      
        1 ggtcgccaag gtcgacgggc aacaccagtc ctccaagtgt atccacgggg gcgcggcgtt
       61 catctggccc gtcatccagg cctaccactt tctcgacctg acgcccctct ccatcgaggt
      121 caacctcaga aacgcgctca gcaagcagaa catccgcgtc gacgtcccct cgcgcttcac
      181 agtcggcatt tccaccgagc cgggcatcat gcagaacgcc gccgagcgcc ttctgggtct
      241 ccagctcgcg gagatacagg agctgtccaa ggacattatc ttcggccagc tccgcctcgt
      301 catcgccacc atggacatcg aggagatcaa caccgaccgc gacaagttcc tcgccaacgt
      361 caccgccaac gtggaggccg aactgaagaa gatcggtctg cgcctgatca acgtcaacgt
      421 caccgacatc aacgacgaat ccggctacat ccaggcgctc ggcaaggagg cggcggccaa
      481 ggctgtcaac gacgcgaaga agagcgtcgc cgaacaggac cgcgacggct ccatcggcga
      541 ggccaacgcg cagagggacc agcggatcca ggtcgcccag gcgaacgccg ccgcagtcga
      601 gggggagaac ctgtcgaaga tcaccatcgc gcagtcggag gccgagcgcc gagagaagca
      661 ggccgagtcc aacaggctcg cggaggctgc ggagaaggtg caggcggcca aggcgctgga
      721 agagtcgtac aaggcggagg aggaggccga gcttgcgcgc gccaggcgcg agaaggccac
      781 tcaggaggcc gacgtgctgg tgcacgccgg aatcgagaag gagagaatcc agattcgggc
      841 cgacgccgac gccgaggaga tgcgccggac ggcggcgggc gaggccgacg ccatcttcgc
      901 gaagatgagc gccgaggcca ggggactccg cgagatcctc gtcaagcagg cggagggctt
      961 cgagcggctg gtcaaagccg cgggcggcga caccgacaag gctatcatga tgatgatcgc
     1021 cgacaagctt cccgaactgg tgaagaccca ggtcgaggcc atcaagggga tcaagatcga
     1081 caaggtcacc gtctgggaca gcgccggagg agccagcgac aagacatcca ccgcgaactt
     1141 cctctccggt ttgatgaaat ccatccctcc gctccaggac atgttcaaaa tggcgggaat
     1201 ggagctgccc gcctacatga agggcggaca gaagaacgaa gcgccctggc ccgttccgca
     1261 cgaggccgga cccgaacgct ggatggccga cgcgggcgaa cccgtcgaga ccgacccgca
     1321 cagggagatg cctccggagg tagaaacgga gcacgcgtag agcgccaacg ctccgcggaa
     1381 ggaatgcagc tgtgtatcct gtagttgtgc gcatgctctg acaaagggga ggtgttccga
     1441 gtgaaaagaa aggttttgtt tgtgttcatc tgtatcgcgc tctctctctt cttcgtggaa
     1501 gtctttggag cgtggggcgt cgggggcgcg ttcgcggagg aactgacgct tatcgagcag
     1561 cagaagctcg cgaggacgct cttcaataag atagccgcca aggacgaaca ggatgttcaa
     1621 gggatcgagc ctctcttcct ggaggttatc gagaaggctc cccgggtccg gcgcgccgag
     1681 gaggcgtact ggatgctcag caacatgtac atgcaggcgt tcgatccgcc cagaaacaaa
     1741 gacgccgtcg cgctgctgga gcagtatctg acgcgctacc cccgctcccg cgtactggac
     1801 gacaagttct ccatgttcac cacccccgga ctcccgcttg tgaaagggcg cctcctttct
     1861 ttgtacgagc aggaggagat gtgggagaag gccgcggccc tctacgacga agtcattccg
     1921 gagccgaaga cagccggcga ggcgctgctg agccatttca tcgggtacgg cagggccatg
     1981 gagcagaccg gacacaaaga gaaggcgata gcggcgtata cggtatttct tgagcgcagc
     2041 ggaaatacgg gggacttcct tgaggagatc gtgaaggatc gcctcaagga gttgggggtc
     2101 gaagtctcct cggacacatc cgttgcggat ggaacggttc cggaagaccc ggggaatctg
     2161 gaatccttcc gcggacggaa cggcgaagtg ttccgtttcc gcgtgcgggg atcggcgctg
     2221 ggtagccttt ggggggacgg ggtctacacg gacgattcga gtctggcgac ggccgccgtg
     2281 catgcggggg tgctccgcca ggaagaagag ggagtcgtcg ccgtgagaat tctgccgggg
     2341 cggaaagagt atgctggcgc cgagcggaat ggcgtcgtct ccgaaagcta cggcgagtgg
     2401 cacggaagtt tcgagttcgt cgaagctgta ggggattcct gaggatcgaa agcggcagca
     2461 cgggcggaga cgatctcgca tggactcgca aaccctcagc tactacgacg acaacgccga
     2521 acggcttgcg cgcgtacgac gccgcggacg gcattgaaag cgcattgaca gccagctaca
     2581 gtccttcgat atccttgcgg cgaagataga gcgttcgctc ttccttggga agggaggcga
     2641 atccgaagtg caggtagaaa cgccgggcgt tctcgtcttt tgcgtcgacg acgaaaagag
     2701 tccaccccac ggaagcggac aggcttctgc gtattgcgtc gatgagcagg tattctccaa
     2761 gacctcgccc ccggcaatcg agcgagactg cgagtcttcc gagtcgaacc gccgggacgg
     2821 aggggtatcg cggcattttt accctcagag cctcggggag attcacaaga aggacgcctg
     2881 ctgcggagag tgtgtagtag ccgcagacgg cgtccttctt gccggtttcg gattcggcga
     2941 cgtagaccga ggcgaagcct ctcttttgat cctgcccggc acgatttcga aaatactcgt
     3001 caagctccgc cacgccgcac acgaacgttt tgcgatcatg cgcgggcgaa agcgggacgc
     3061 atctccagct cattccggct gttttgccgc atcgtagcgg gccgctgcaa cacgcaacct
     3121 gggggagggc tccctttcgg gggcgcagag gtcgagggcc agcgcccggc tgtcctcttc
     3181 gcacaggcgc agcagcgcgt cttcttcgat ggtgcggcgg gcagcctccg ccgctgcgga
     3241 gacgacgaag tcggagaggg tgcgcccttg cagggccgca gccctggcga ggtgtttctt
     3301 gagatcggag ggaatgcgcg cttcgatcct gctttctttc actgctgttt tctccatgag
     3361 gaacacctcc gcgataattg tacggctctt gtacggaagt gtcaacgata atcgcatgcg
     3421 acagggtaaa tggagtctcc atcgctgtag agtgaaagga gcccggatct ctccgggctc
     3481 ctggtgcttc ttacataagc tccttcagcg cggccagggc ggcttcgtag tccggctcgg
     3541 cggtctgctc cgggacgatc tggacgtact ggacgacgtc ctccttgtcc acgatgaaga
     3601 tggagcgggc gaggaggcga agctccttca tcaggacgcc ccagttggtt ccgaaggatg
     3661 cgtctctgtg gtccgagaga gcgacggcgt tctctatgcc gccggtggcg cagaagcgct
     3721 tgatggcgaa gggaaggtcc atcgaaatgt tgagcaccgc cacgtcctgc ggctgcttcg
     3781 atgcgtcctc gttgaaccag tgaatctgga ggtcgcacac gggggtatcc agcgaggggg
     3841 tcaccgatat gaccttgaac ttccccgcgt agtccttcag cgtcttcggg gccatcgtct
     3901 ggtcgagcac gaggaaatcc ggcgctttgt ctcccggttg caggccggga ccgacgagcg
     3961 tcatcggatt ccccttcatt gtggttattc ctcttctttc catagcgtat tcacctccga
     4021 aataatttct gtggcttatt gtatcacaaa aagagggcgg aggggaatat acccccccgc
     4081 cctgtatggc gcgaataaat gaggcgactt ttcgactctc tacagctctt tgccccactt
     4141 tccggagcgc atgacgccga tgagcagcac cgggacgatg agcagcggaa aggagaggac
     4201 tccggtgtac ccgtaccctt tggcgatcag cggaataaga ccgaagagcg cgatgcccca
     4261 cgtgatgagg acgtagatca gggatgctgc gatgttcact tttctgtctc cggccactgt
     4321 cgatcctttc attttccccc acaggcccac gatgcgccgc gcgccgccga aaatgaggct
     4381 gacgccggtg gagatcacgg cgaggaagat gagcagcgag acgatggcgg tgcctatcgc
     4441 gccgccgccg ccgtgcgcgg ccacgaagag cacgggaact ttctcggtaa ggatggcggg
     4501 gtagtggagg agtatgcccg ccgtcgcaag ccagagcact cccgcgttga cgatgaagcc
     4561 ccagatcgag gccttcttcg cgtcctctcg gcttttcagc gcgtcggcca ccgcgatgta
     4621 ggcgcccatc gcgcacgcct ggaagcccgc gtatttgagc gacgcccaga gcgcgtcgag
     4681 gaagctcgcg ggcgcgggag cctccttgag gaccgcgagc agcttcggga agttgacgat
     4741 caggtttccc acgtagatga gcagcattcc ggcgatgatg atcaccgcca ttgtggaggc
     4801 ggccttcctg acaaggtccg cgccgaagat ggtgaggaag aagatgcaga cggcgacgat
     4861 cacggtgttc atgacatagg gagtccccag gatctgctgc atcaccgttc cgccggtggc
     4921 gaaggccacc gccgaggcga tgagcaggat gaggatgtac atgacttcat aggcgttgga
     4981 gaagagcgcc tcgaagggtt tgaagtattt gtttccccac tcgcggtagt cgaaggtgca
     5041 gtaaatagcg gcgaaggtcc atgcgtagta gagcaccgcg gtgacgatgg cgatggagag
     5101 gacgggcagg aagagcgcgt accacccgta ggacacgaaa tactggacta cctgcacgcc
     5161 gctggcgaac ccgccgccga agtgggtggt gaaccagacg aacgccactg cgagaatgcc
     5221 gaacctgttc ttgtcgatca taatctcttc tcctctcgtg cttacagtaa ggtatgagtg
     5281 cgccgggggt gtcctctaca ggccgccccc gttgttgccg tcagtttccc agcagcttgc
     5341 cggggttcag aatgccctgc gggtcgaagg atcgcttgat cccgcgcatg atctcc
//
LOCUS       DAOPKU010000161         5394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16429_length_5394_cov_3.598989, whole genome shotgun sequence.
ACCESSION   DAOPKU010000161 DAOPKU010000000
VERSION     DAOPKU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16429_length_5394_cov_3.598989"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK849_06330"
     CDS             complement(<1..448)
                     /locus_tag="PK849_06330"
                     /inference="COORDINATES: protein motif:HMM:NF020425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HPE65780.1"
                     /translation="MSFSTGGLFRRESLEAASLFLQSGSWDAVRKEIMAKNLLQARTL
                     STLQRECREVLSRLRLLNGEELRFLVEAEYREQGHLLWIAVCRRYPFVRDFAVEVLRE
                     RYITLKADVRYEEFDAFFNAQSEAHPQLHEISSTTRKKLRQVLFKIL"
     gene            859..1350
                     /locus_tag="PK849_06335"
     CDS             859..1350
                     /locus_tag="PK849_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65781.1"
                     /translation="MSRTYAEITAVVLFLSACIILPVSWWLSGETNAFRERGVPTMVR
                     IIRFEEVASGEDGTRRIAVAERVTDGRRPAGEDLLASHILMDEITEVGSLHLGQELEV
                     AVLPDAEFFMTPPGYTHWFITRRSLDRHTRQPISEWGWNSALVLTTLSLLLGAYSASK
                     ERK"
     gene            1377..1499
                     /locus_tag="PK849_06340"
     CDS             1377..1499
                     /locus_tag="PK849_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65782.1"
                     /translation="MTAEDAEFDTAWTRLDDLYAPFVQQDGEYPLHDLLGVKRT"
     gene            1528..2214
                     /locus_tag="PK849_06345"
     CDS             1528..2214
                     /locus_tag="PK849_06345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPE65783.1"
                     /translation="MESGYYEKKLNAEKLYRVYQTKIPQVRDYLRSEIEFVREHLGGN
                     ETALELGAGYGRIMKELSPSLGRITGIDVSEQSVALGREYLGDLANCTIETMDAHTLA
                     FDETFDVVLCLQNGLSAMKGDPENLVRRSLRVLAPGGTAFFSSYSPKFWEHRLAWFQE
                     QAGKGLLGEIDMENTGNGRIVCKDGFTATTFDREDMERLGKASGHPYRVEEVNNSSIF
                     LTLRNEAPRS"
     gene            2272..3171
                     /locus_tag="PK849_06350"
     CDS             2272..3171
                     /locus_tag="PK849_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HPE65784.1"
                     /translation="MHRTPPAAEGFIQFKGYKIWYRVVGEREEPGKLPLLCLHGGPGA
                     PHDYLEPLEALASGGRRVFFYDQLGCGNSDRPSDPSMWTVGLFLEELGVVRKALGLDR
                     VHILGQSWGGMLAMEHALTRPSGLASMIVADSPASMLQWVSEANLLRAELPPETQRTL
                     LEHEAAGTTDAPEYQEAMNVFYNRHLCRLDPWPECLLRAFEKTGFEVYTSMNGPSEFH
                     VTGKLKEWDIVERLPEIDTPTLVIGGRYDEATPAITETVHRGIRGSEWVIFEESAHMP
                     HLEETERYLRVVGDFLNRIEARL"
     gene            3201..3722
                     /locus_tag="PK849_06355"
     CDS             3201..3722
                     /locus_tag="PK849_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPE65785.1"
                     /translation="MREEMSCKEQDKTLAAVCGLYCEACSLFIATKEDPARLTALAKM
                     FQLPEEEMRCYGCRSEKRGPYCTTCRMFVCAAERGIDFCGDCDEYPCDMLKAFQAERP
                     HRAELWEDLERIRDVGYAQWMTEKREHYACPQCATLNSAYDLKCRKCGAEPSCAFTAR
                     HREVIEEAMKRAR"
     gene            complement(3824..4345)
                     /locus_tag="PK849_06360"
     CDS             complement(3824..4345)
                     /locus_tag="PK849_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65786.1"
                     /translation="MESTAATEGTQLVVRESEESGEAELTLNIARNGVLFQYPHNNNF
                     IFSNKKRADGILFLQRENDWEVMIVELKKTVKAKEWMKIKEQWHGAWLHALALSGVLE
                     AHFPNPPRFLVAYRKDGIGENAPDPILLKSPEYALAFIEWRKGRAALNEIGEAAFEKA
                     PLDETGRGELRIP"
     gene            complement(4350..>5394)
                     /locus_tag="PK849_06365"
     CDS             complement(4350..>5394)
                     /locus_tag="PK849_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE65787.1"
                     /translation="RQTLCDELFGENAVRLRELRITDFVRERPLSLRWNKDAAEGSRY
                     SSGEHYVRFPLPSSPPPRAEVYRMIQYLTWKLIMDDLSAPLYPPRSPVKTVPPGEILY
                     LPAARTGFVLLRKALVSAALSMGGESVPLLLPVRRFVQRLLTLSFKEKNRFSAFADIL
                     ERRILRGHIHPDGAPLPEYRYSPQGQDNEALPLWRTSSLVTELAPLLVFLRGNDDFRS
                     LIIEEPEAHLHLEIQRELARLLVRLVNSGLPVWITTHGDTFFQQVSNLVKASAIDDAS
                     LSRLDLEPEESIQPERVSAWHFQRVSASSGRDRTEISPLHADHDGIAATAFNAAIAEL
                     TRQTLDLSPDDEA"
BASE COUNT         1187 a         1526 c         1630 g         1051 t
ORIGIN      
        1 ggagaatttt gaagaggact tgccgcagct tcttcctcgt cgtggagctg atttcatgga
       61 gttgcggatg cgcctccgat tgcgcgttga aaaaggcgtc gaactcctcg tagcgcacat
      121 ccgctttcag agtgatatag cgctccctga gcacttcgac ggcgaagtcg cggacgaagg
      181 ggtagcggcg gcagacggcg atccaaagga ggtgtccctg ctcccggtac tccgcttcga
      241 ccaggaaacg cagctcttcg ccgttgagca accgcaaccg ggagaggacc tcgcggcact
      301 cccgctgcaa cgtgctcaag gttctggcct ggaggagatt ttttgccata atctccttcc
      361 ggacagcatc ccacgaaccg ctttgaagaa acaaagaagc cgcctccaac gattcccggc
      421 gaaaaagacc tcctgttgaa aaagacatgg cgtattccga atttcccatt tatcggttga
      481 catacccttc gttttaaaat acaaatattt tccagtgcgc tgcatactgt gcacatgagg
      541 ctcatccgta ctttccaaac ttcagattcc ggcttttgga ttctccggcc atacagacac
      601 aaaaaaggcg gaaatggcag gccgtccaga tctacgaaaa tataatacca ttatcttcgt
      661 attatagaaa aattgtaccg ggaaatggag ttttgaaagt tgggatgtga atgtgtacgt
      721 acgatacctt acaaatgccc tctacgtcaa actctgtttg caaaaactca aatcctcggg
      781 ctcgctttcc gaagtcggca agtacaagtg atacaataca caaagaagcc atcccaggcg
      841 acgatacgga ggggaaacat gagcagaacg tatgcggaga tcactgcggt cgtcctcttt
      901 ctatccgcgt gtatcatatt gccggtctcc tggtggctct ccggggagac gaacgccttc
      961 agggagcgcg gagtaccgac gatggtccgc attatccgct tcgaggaggt tgccagcggg
     1021 gaggatggaa cacggagaat agccgtcgcc gagagagtga cggacggacg gagacctgcc
     1081 ggggaggatc ttctggcgtc ccacattctg atggacgaga taacggaggt gggctcgctt
     1141 cacttggggc aggagttgga agtggccgtc ctgcccgatg cggagttttt catgacgcct
     1201 ccgggctata cccactggtt catcacgcgc cgttcgctgg accggcatac gcggcaaccc
     1261 atctccgaat ggggctggaa ctccgcgctg gttctgacga cgctttcgct gcttctgggg
     1321 gcgtactcgg cttcgaagga gaggaaataa tcaagcagaa acggtggatc ggctacatga
     1381 cggcggagga cgcggagttc gacacggcat ggacgaggct cgacgacctg tacgcgccct
     1441 tcgtccagca ggacggcgag tatccgctcc acgacctcct cggcgtgaag aggacataat
     1501 ttcgcttccc gcaaaggagg aaaacacgtg gagagcggct actacgaaaa gaagctgaac
     1561 gcggagaagt tgtaccgggt ctaccagacg aagattccgc aggtgcgcga ctaccttcgg
     1621 tcggagatcg agttcgttcg cgaacatctg ggcgggaacg aaacggcgct ggagctgggc
     1681 gcgggctatg gaagaatcat gaaggagctg tcgccctccc tggggagaat cacgggcatc
     1741 gacgtctcgg agcagtccgt cgcgctcggg agagagtatc tgggagacct cgccaactgc
     1801 acgatcgaaa cgatggacgc gcacaccctg gcgttcgacg agacgttcga cgtcgtgctc
     1861 tgcctgcaga acggactgtc ggcaatgaag ggcgatccgg agaacctggt ccgccggtcg
     1921 ttgcgcgtgc tcgcgcccgg agggacggcc ttcttcagca gctacagccc gaagttctgg
     1981 gagcaccgtc tggcgtggtt tcaggagcag gcgggaaagg gcctgctcgg ggagatcgac
     2041 atggagaaca caggaaacgg ccggatcgtc tgcaaggacg gcttcaccgc gaccacgttc
     2101 gaccgcgaag atatggaacg cctggggaaa gcctcggggc atccctacag ggtagaggaa
     2161 gtgaataact cgagcatctt cctgacactg cgcaacgagg ctccgcgctc gtgaagcgca
     2221 ccgcgcctca tgcgagcgat agcgatcatc atgaaatagg agggggtacg aatgcaccgg
     2281 acaccgccgg ccgcagaggg attcatccag ttcaaggggt ataaaatctg gtaccgcgtc
     2341 gtcggggagc gcgaggagcc ggggaaactg ccgcttctct gtctgcacgg cggccccggc
     2401 gcgccgcacg actatctcga accgctggag gcgctggcct caggcggacg gagagtgttt
     2461 ttctacgatc agctggggtg cggcaattcg gaccgcccga gcgacccgtc gatgtggacg
     2521 gtcggtctgt ttctcgaaga actcggcgtc gtccggaaag ccctcggact ggatcgcgtc
     2581 catatcctgg gacagtcctg gggaggcatg ctcgcgatgg agcacgccct cacgcggccg
     2641 tccggactgg cgagcatgat cgtcgccgac tcccccgcca gcatgcttca gtgggtttcc
     2701 gaggcgaacc tcctgagggc ggagcttccc cccgaaacgc agcggacgct tctggagcac
     2761 gaagccgccg gaaccacgga cgctcccgaa taccaggagg cgatgaacgt cttttacaat
     2821 cgccacctct gccgcctcga cccgtggccc gagtgcctcc tcagagcgtt cgagaagacg
     2881 ggcttcgaag tctacacctc catgaacggg ccaagcgagt tccacgtcac cggcaaactc
     2941 aaggagtggg acatcgtgga gcgccttccg gagatagaca cgcccacgct cgtcatcggc
     3001 ggccgctacg acgaggctac ccccgccatt accgaaacgg tgcatcgcgg catacgcggc
     3061 tccgaatggg tgatcttcga ggagagcgcg cacatgccgc accttgaaga gacggagcgc
     3121 tacctgcgcg tggtcggcga ctttttgaac aggatcgagg cgcgcctgta gtcgcgcaac
     3181 attctcaagg agggaacatc atgcgtgagg aaatgagctg caaagagcag gacaagacgc
     3241 tcgccgcggt gtgcggcctg tactgcgagg cgtgttcgct ttttatcgcc acgaaggagg
     3301 acccggcgcg gctgaccgcg ttggcgaaaa tgttccagct tccggaggag gagatgcgct
     3361 gttacggctg tcgctcggag aagcgaggcc cctactgcac aacgtgccgg atgttcgtat
     3421 gcgccgccga acggggaatc gacttctgcg gcgactgcga cgaatacccc tgcgacatgc
     3481 tcaaagcctt ccaggccgaa cggccgcacc gcgccgaact ctgggaggat ctggagcgga
     3541 tccgcgacgt cgggtacgcg cagtggatga cggaaaaacg ggagcactac gcttgtccgc
     3601 agtgcgcaac cctcaactcc gcctacgacc tgaagtgcag gaagtgtggc gcggagccga
     3661 gctgcgcctt taccgccagg cacagggaag ttatcgaaga ggccatgaag cgggcgcgat
     3721 agaagtctcg cccatccgga caataaacgc agatcgcacc gctaaaaata cgcagcgcga
     3781 tctgcgttta ttcaatcttc ctcgctccgg attccggcgt ctttcatgga atacgcaatt
     3841 cgcctcttcc cgtttcatcg agaggcgctt tttcaaaggc tgcttctccg atctcgttca
     3901 aggcggcgcg ccccttcctc cactcgatga aggcaagagc atactctggc gatttcaaca
     3961 ggatcggatc gggagcgttc tcgccgatac cgtccttgcg gtacgccacc agaaaacggg
     4021 gagggttggg aaaatgagcc tccagaacac cggagagggc cagagcatga agccatgctc
     4081 catgccactg ttctttgatc ttcatccact ccttggcctt cacggtcttt ttgagttcga
     4141 cgatcatgac ctcccagtca ttctctctct gcaagaagag aatgccgtcc gcgcgctttt
     4201 tattcgagaa gatgaagttg ttattatgcg ggtactgaaa aagtactccg ttccgtgcga
     4261 tattcagcgt cagttcggcc tcgccgctct cttcggactc tcgaacgacg agttgcgttc
     4321 cctccgttgc tgcggtgctt tccactcgtt cacgcttcat cgtccgggct caagtcaaga
     4381 gtttgccgag tgagttcggc tatggcagca ttgaaggcgg ttgcggctat tccgtcgtga
     4441 tcggcgtgca acggcgaaat ttcggttcgg tcgcgaccgg acgaggcgga aactctctgg
     4501 aaatgccacg cagaaacccg ttccggctgt atgctctctt cgggctccag atcgagtcgg
     4561 gagagagatg cgtcatctat ggccgacgcc ttgaccaggt tgctcacctg ctgaaagaaa
     4621 gtatctccat gcgtcgtgat ccagacggga agaccggaat tcaccaatcg aaccagaagg
     4681 cgggcgagct ctctctgtat ttccagatga aggtgcgctt ccggttcttc gatgatcaaa
     4741 gagcgaaaat cgtcgttccc tcgcagaaag acaagaagcg gagcaagctc cgtaacaagc
     4801 gacgacgtcc gccaaagagg gagcgcttcg ttatcctggc cttgaggact gtatcggtac
     4861 tccggtagcg gagcgccgtc cgggtgaatg tgtccgcgca gaattcggcg ctccagaata
     4921 tccgcgaagg cggagaacct gtttttctct ttgaaggaaa gggtaagaag gcgttgaacg
     4981 aaacgcctga cgggcagcag aagcggcacg ctctcgcctc ccatgctcag cgctgcggaa
     5041 acaagagctt ttcgcaagag cacgaaccct gttcgtgccg ccggcaagta gaggatctcg
     5101 cccgggggaa cggttttcac cggagatcgc ggaggataga gaggcgcgct caaatcgtcc
     5161 atgatcagct tccaggttaa atactggatc atcctgtaga cttcggctcg gggaggagga
     5221 ctcgacggca gcgggaagcg gacatagtgt tctccgctgg aataccggct tccctcggcc
     5281 gcgtctttgt tccatcgaag ggaaagaggc cgttcgcgaa caaaatccgt aatgcgaagc
     5341 tcgcgaagcc gcactgcgtt ttccccgaaa agctcgtcgc agagagtctg tcgc
//
LOCUS       DAOPKU010000162         5383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16481_length_5383_cov_3.786787, whole genome shotgun sequence.
ACCESSION   DAOPKU010000162 DAOPKU010000000
VERSION     DAOPKU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16481_length_5383_cov_3.786787"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PK849_06370"
     CDS             complement(<1..261)
                     /locus_tag="PK849_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HPE65788.1"
                     /translation="MATVGIKGYAYCLNHAPETGYHYGNTPHVERETKGETEFLKELP
                     SFLQTYEDARDYAPNQAYIGGISLEKLEQTPQPWYTNRLTGSS"
     gene            complement(341..1651)
                     /gene="grdB"
                     /locus_tag="PK849_06375"
     CDS             complement(341..1651)
                     /gene="grdB"
                     /locus_tag="PK849_06375"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(602..604),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HPE65789.1"
                     /translation="MVTYRIIHYINQFYAGIGGEEKADITPEIREGIVGPGQALKAAL
                     GAEAEIVATVICGDSYFASNMEKASEEILGMMSKYKPDAVVAGPAFNAGRYGTACGAL
                     GEAVVKKLGIPVVSGMYPENPGVEMYKKSFYIVETPDSAAGMRKAVPAMAKLVLKLLK
                     GENLGTPSEEGYIERGIRKNKFYEKLAAERAVDMFTAKLKGEPFVTEYPMPVFDRVAP
                     NPAIKNMKDAVIALVTSGGICPKGNPDHIEASSASKFGEYDISGVMDLTCDSYCTAHG
                     GYDATYADQDADRVLPVDVLRDMEKEGVFKKLHNKFYTTVGNGTAVASAKRFAEEIAK
                     RLIADGVTAVILTSTUGTCTRCGATMVKEIERAGLPVVHICTIVPISLTVGANRIIPA
                     VAIPYPLGDPSRTMAEEKVIRRHLVEKALKALQTEITEQTVFTD"
     gene            complement(1666..2142)
                     /gene="grdA"
                     /locus_tag="PK849_06380"
     CDS             complement(1666..2142)
                     /gene="grdA"
                     /locus_tag="PK849_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_133955713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2011..2013),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HPE65790.1"
                     /translation="MGKLAGKKVLLLGERDGVPGPAMETCLKNSGADIVFSVTECFVU
                     TAAGAMDLQNQQRIKDAVEKYGPENVVVILGSSDAEGAEIYAETVSNGDPTYAGPLAG
                     VQSGLAVFHVFEQEIRDECDANAWEEQISMMEMVLDPEKLSEAVSGIRGQFSKYTL"
     gene            complement(2199..3485)
                     /locus_tag="PK849_06385"
     CDS             complement(2199..3485)
                     /locus_tag="PK849_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HPE65791.1"
                     /translation="MKLELHKVKVNKLAWGDKTFAKGGVLTVNKKELLSVLSGDERLA
                     KVDVDFALPGESVRILPVKDVVEPRFKIEGPGGIFPGMVSDVDSVGEGKTLVLSGAAV
                     VTTGKIVRFQEGIIDMKGPGAEFTPYSQTCNVVVILENVEGIEKHDYETACRMAGLKT
                     AAYLAEKCLGASVDKVETYETPSLKDAMHAHPGLPKVAYLYMLQSQGLLHDTYLYGVD
                     VKKILPTFLNPTEVMDGAIVSGNCVSACDKNNTYVHQNNPIIHNMFKRHGKDFNFLGC
                     IVTNENVTLADKKRSSSYSIKLAKTLGVDGLLISEEGFGNPDADLIMNCWKAERAGIK
                     TTLLTDEYAGQDGASQSLADSCPEGDACVTAGNANEVIVLPPMEKVIGVPEEANVIAG
                     GWQGSLAADGTITVELQAILGSTSELGFTKLGAYTI"
     gene            complement(3522..3842)
                     /locus_tag="PK849_06390"
     CDS             complement(3522..3842)
                     /locus_tag="PK849_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPE65792.1"
                     /translation="MVELTKENFDVEVKESALPVLVDFWGPKCGPCMALLPNVHTMAD
                     EYEGKVKFCSVDVSENRRVAIANKVMGLPTFLFWKDGAEVTRISGGDVTLEKIKENVE
                     ALLK"
     gene            complement(3867..4265)
                     /locus_tag="PK849_06395"
     CDS             complement(3867..4265)
                     /locus_tag="PK849_06395"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HPE65793.1"
                     /translation="MLVTNNPLLNEQISSRDFVEGNSLDVLIRTRNLIHKGWVLLTHP
                     LYGNLRPHQHPYRSILMERASDSSSSVDPLSVEYIENAIAIYSAQSSRILSVEGMPED
                     VRQDFAFIDADLMKESLSRYRMFIREVSVN"
     regulatory      complement(4385..4487)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(4652..>5383)
                     /locus_tag="PK849_06400"
     CDS             complement(4652..>5383)
                     /locus_tag="PK849_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPE65794.1"
                     /translation="EFYLKPDFSKITGASISAALGQAFFSLSLGMGCMITYGSYVDRK
                     TALPGAAIQVCFIDTMVAILAGLVIFPAVFAFGVDAGAGPGLTFVTLPSVFAKMPGAM
                     IWSGLFFLLLFIAALTSAISLLEVVSAYFIDKGWSRPQAAIIMGLLIFVLGIPSAMSL
                     AGAPKVAGKDFLDAMDFISSNVLLPLGGVFISLFVGWFWTSDAEKEVTNEGTLTFGLM
                     SMWIWVCRVIAPAAILYIFYTGLKW"
BASE COUNT         1191 a         1530 c         1416 g         1246 t
ORIGIN      
        1 gctcgagccg gtcagcctgt ttgtgtacca gggctgggga gtctgttcca gcttttcaag
       61 agagataccg ccgatgtatg cctggttggg cgcataatcc ctggcatctt catacgtctg
      121 taaaaaagag ggcagttcct tgagaaactc cgtctctccc ttcgtctctc gctcaacgtg
      181 cggcgtgttt ccgtaatgat atcccgtttc gggagcgtga ttcaggcagt aggcatatcc
      241 tttgattcct acggtggcca tgacgttcca acctccttgt gcttttctcg cacggccggt
      301 tccggggttc cccccggttc cggccgtata cgcaacagaa tcagtcggta aataccgtct
      361 gttccgttat ctccgtttga agtgccttca aggctttttc aacaagatgg cgacggatta
      421 ctttttcttc cgccattgtc ctcgatgggt cgccgagagg atagggtata gcaactgcgg
      481 ggatgattct gtttgctcct accgtgaggg agatggggac tatggtgcag atgtgcacca
      541 caggaagacc tgcgcgttcg atttctttga ccatcgttgc accgcaacga gtgcaggtgc
      601 ctcaggtgga tgtgaggata acggcggtca ctccgtccgc gatcagcctt ttcgcgattt
      661 cttcggcgaa gcgtttggca ctggccacgg cagtaccgtt gccgaccgta gtatagaact
      721 tgttatgcag cttcttgaag acgccctctt tttccatatc gcgaagaacg tctaccggaa
      781 gcactctgtc cgcatcctga tcggcgtacg ttgcgtcata accgccgtgg gctgtgcaat
      841 acgaatcgca tgtaagatcc atcacgcccg agatgtcgta ttcgccgaat ttgctcgcgc
      901 tggaggcctc gatatgatcg gggttccctt tggggcagat accgccggaa gtgacaagcg
      961 cgatgaccgc gtctttcata tttttgatcg cgggattggg ggccacgcgg tcgaagacgg
     1021 gcatcggata ttcggtgaca aacggctctc ccttgagctt tgcggtgaac atatcaaccg
     1081 cgcgctctgc agccagcttc tcgtagaact tgttctttct gatgccgcgc tcgatgtatc
     1141 cttcctcgga aggcgtaccg agattctcac ccttgagcag cttcagaaca agctttgcca
     1201 tcgcgggaac agccttccgc atgccggcgg cgctgtcggg agtctccacg atgtaaaaac
     1261 tcttcttgta catctcgacg cccgggttct ccgggtacat accggagacg accgggatac
     1321 caagcttctt cactacggct tcgccgagag cgccgcacgc agtgccgtac cgtccggcgt
     1381 tgaaagcggg gccggcgaca accgcgtcgg gcttgtactt gctcatcatc ccaagaattt
     1441 cctcgctcgc cttctccata ttcgaggcga aatacgagtc gccgcagatg acggtagcca
     1501 cgatttcggc ttcggcccca agagctgcct ttaaagcctg tccggggccg actatacctt
     1561 cgcgaatctc cggcgttatg tcggccttct cttcaccgcc gattccggcg taaaactgat
     1621 ttatatagtg tatgatccgg taagtcacga tgacgacctc ccctcctaca gggtgtattt
     1681 actgaactgc ccgcgaatgc cgctgacggc ctcggacagt ttctcgggat caagcaccat
     1741 ttccatcatg ctgatctgct cttcccaggc gtttgcatcg cactcgtccc gaatctcctg
     1801 ttcaaaaaca tgaaatacag cgagtccaga ctggactccg gccaagggac ctgcatacgt
     1861 cggatctccg ttgctcaccg tttcggcgta gatttctgcg ccttcggcat cggacgatcc
     1921 gagaatgacg accacgtttt cagggccgta cttttcaaca gcgtccttga tgcgctgctg
     1981 attctgcaga tccatggctc ctgcggcggt tcagacgaag cattcggtta ccgagaagac
     2041 gatgtcggcg ccgctgttct tcagacacgt ttccatagcg gggcctggaa cgccgtcgcg
     2101 ttcaccgagg agaagcactt ttttgcctgc caatttcccc atgctcttgc acctccttgt
     2161 cgaaaaactt gtatccgggt attcccggtt tggtcacatc agatggtgta cgcgcccagc
     2221 ttcgtgaaac caagctcgct cgtggaaccc agtattgcct gaagctccac tgtgatcgtc
     2281 ccgtcggcgg cgagagagcc ctgccagcct ccggcgatga cgttggcctc ttcggggacg
     2341 ccgatgactt tctccatcgg aggaagcaca atcacttcgt tggcgtttcc ggcagtcacg
     2401 cacgcgtcgc cctcggggca ggaatcggcg agcgactggc ttgcgccgtc ctgtccggcg
     2461 tactcgtcgg tgagaagcgt tgtcttgatt ccggctcgct ccgccttcca gcagttcata
     2521 atgaggtcgg cgtcggggtt gccgaaacct tcctcggaaa tgagcagccc gtccacgccg
     2581 agcgtcttcg ccagcttgat cgaataagaa gagctcctct ttttgtcagc gagggtgacg
     2641 ttctcgttgg tgacgatgca gccgaggaag ttgaaatctt tgccgtggcg tttgaacatg
     2701 ttgtggataa tcgggttgtt ctgatgcacg tatgtgttgt ttttgtcgca tgcggaaacg
     2761 cagttgccgg agacgatggc gccgtccatg acctcggtcg ggttgaggaa cgtgggaaga
     2821 atcttcttca catccacacc gtagagatac gtgtcgtgga gcagcccctg cgactggagc
     2881 atatacagat acgcgacctt cggaagcccg ggatgcgcgt gcatggcgtc tttcagcgag
     2941 ggcgtttcgt aggtttcaac tttgtcgacc gaagcgccaa ggcacttctc ggcaagatac
     3001 gcggcggtct tcagtccggc catcctgcat gccgtctcgt agtcgtgctt ttcgattccc
     3061 tcgacgttct ccaggatcac gacgacgttg caggtctgag aatacggagt aaactccgct
     3121 ccgggtccct tcatgtcgat gatgccttcc tggaaacgga cgatcttccc cgtggtcact
     3181 acggccgctc cggagaggac gagcgtcttg ccctcgccca cggaatcgac atcggagacc
     3241 atgcccggga atattccgcc cgggccttcg atcttgaatc gaggctccac gacgtctttt
     3301 accggaagaa tgcggacgct ctcgccggga agcgcgaaat caacgtccac tttcgcaagg
     3361 cgctcgtccc cggaaagaac ggagagaagc tccttcttgt tcaccgtcaa tactccgccc
     3421 ttggcgaagg ttttgtcgcc ccaagcaagt ttgtttactt ttaccttgtg cagctcaagt
     3481 ttcatggctg tctgtctccc cccttttaca ggcgtcgaag gctacttcaa gagagcctcg
     3541 acgttctcct tgatcttctc caacgtgaca tctccgccgc tgatacgggt cacctccgct
     3601 ccatccttcc agaacaggaa ggtaggaagc cccataacct tgttcgcgat cgccacccgg
     3661 cgattttccg atacgtccac cgagcaaaat ttcaccttgc cctcatactc gtccgccatg
     3721 gtatggacgt taggaagcag cgccatgcag ggtccgcatt tcggccccca aaagtcgaca
     3781 agcacaggaa gcgcgctctc cttgacttcg acgtcgaaat tctccttcgt caattcaacc
     3841 atttctcttc acctcctttt agagactcaa ttcaccgaaa cttcccgaat gaacatacga
     3901 taccgggaaa gactttcctt catcaaatca gcatcgatga atgcaaaatc ctgacgcaca
     3961 tcctcaggca ttccctcgac cgagaggatt cgtgacgatt gtgcggaata aattgcaatg
     4021 gcgttttcga tatactcaac ggagagagga tcgacggaag acgatgaatc cgatgcccgt
     4081 tccatcagga tagaacggta tggatgttga tgcggacgaa gatttccgta aagaggatgc
     4141 gtaagaagaa cccatccttt gtggatgaga tttcttgttc gaataaggac atcaagggaa
     4201 ttcccttcga caaagtcgcg ggaagaaatt tgttcattca gaagcgggtt atttgtgaca
     4261 agcattctgc gacagaacat ccggctgcct cctcaaaagt agagcacaga taagacattc
     4321 tctgtcgcat agccccaaag gaaagagaaa aaaacaaaca ggggagctac tctattcaga
     4381 gacccgtccg accgcaatcc tttcgcctga gagtttccac cctatagagg gtattgcccc
     4441 ttcggcgctc tccgcaaacg caggaaagtc tctcttgcgg tgttcatccg gaaacactct
     4501 tcattttaca caatgaaaaa aaggcgtcaa gccctttttg tgaaagacgg agcaaaaaaa
     4561 acgaaaatgc cggaatctct tcggattccg gcattctcac ctcgacaaag tacgaagcga
     4621 aatacacttc gtactgcagt tcagaggacc gttaccactt caaaccggtg tagaagatgt
     4681 acagaatcgc tgcgggagca ataacacggc aaacccagat ccacatgctc atcagaccaa
     4741 aggtgagcgt gccttcgttg gttacctctt tctccgcatc ggaggtccag aaccagccta
     4801 cgaagagcga gatgaaaact cctcccaacg gaagcaggac gttcgaggag ataaagtcca
     4861 tcgcgtccag gaagtccttt ccggcaacct tcggagcccc tgccagagac atggcggaag
     4921 gaatcccaag gacgaagatc agcaggccca tgatgatcgc agcctggggt ctgctccacc
     4981 ccttgtcgat aaagtatgcg gaaacaactt ccagcagaga aatcgcagaa gtcagcgccg
     5041 ctatgaagag aagcaggaag aagagtccgg accagatcat tgccccgggc atcttcgcaa
     5101 aaacggaggg cagcgtaaca aacgtcagtc cgggacctgc gccggcgtcc acaccaaagg
     5161 cgaagactgc ggggaatatc acaagtccgg caagaatagc caccatggta tcaataaagc
     5221 agacctgaat agcagccccg gggagcgccg tcttcctatc gacatagctt ccataggtga
     5281 tcatgcaccc catgccgagg gagagcgaga agaacgcctg accaagagcg gccgagatgc
     5341 tggctccggt gatcttggaa aaatcgggct tcaggtagaa ctc
//
LOCUS       DAOPKU010000163         5344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16631_length_5344_cov_4.450180, whole genome shotgun sequence.
ACCESSION   DAOPKU010000163 DAOPKU010000000
VERSION     DAOPKU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16631_length_5344_cov_4.450180"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..678
                     /locus_tag="PK849_06405"
     CDS             298..678
                     /locus_tag="PK849_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPE65795.1"
                     /translation="MFLFRLLLKAAPLLLFFLFSKILKDMLRGAQGSPGPSGGARGRS
                     SSGGGAAYTPPPGGKRDPYTVLGCSPSSSNEEIKKRYRELLAKYHPDKFIGQNLDEDF
                     VALASRKFQEIQEAYGRIRSARGI"
     gene            873..2534
                     /locus_tag="PK849_06410"
     CDS             873..2534
                     /locus_tag="PK849_06410"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPE65796.1"
                     /translation="MSTLELFTGPESFSRIKSQARTTIESAFFGNNVVPVSNLKEAYE
                     LARVSPGTVELTGMPVYRPEGQGLPPRSNVLLMNDGAVVGRCAAARRIVGEPGVNMGD
                     FAAVLREAVYNSRFRTFYSAEAYIGLEGDFMVKAHLLAPKNHENLVYNWLLNFQYLNR
                     TYLDMYAASRPLPDGDIFMFADPDWTSPDYPLGLAFFDPLHNCAAVLGMRYFGELKKG
                     TLTLAWGTAVRNGFASCHGGLKRYNLKGGRSFVAAVFGLSGSGKSTITHARHGNKYDI
                     TVLHDDAFVINVKEKYAIALEPSYFDKVQDYPMGCEDNKFIITLQNCGVTRGEDGLLY
                     AVTEDLRNGNGRAIKSKLWSPERVDRIDEPIDAVFWLMKDSSLPPVLRLKGSDLAAVL
                     GATLATKRTTAERLAPGVDPNALVIESYANPFRTYPLATDYERFKALFDDGVACYILN
                     TGEFMGKKVKPADTLGILEALVEGKAEFVPFGPFSDMETMALDRFPIDFTDEQYRREL
                     FSRMRDRLAFVTSRETEKGGMDRLPPEAVAALRKALDEMGYPVQE"
     gene            complement(2563..3120)
                     /locus_tag="PK849_06415"
     CDS             complement(2563..3120)
                     /locus_tag="PK849_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65797.1"
                     /translation="MTEAFLFPLRLHLLCAPSHRRGEYLLERKFAQAFAASNGIPLDF
                     DALMATLREWCASQGVVRNGQTASFSGKSADKKYSGTATRFRDELSILIHVDGEGRKR
                     FRILGLWNDFSWLVLYQEPLLGEWRSWPGAAKDPEGMEKDRTDERSAREGFEWVCRRR
                     IISRARLLRGEEVTTEYYSPSYRKR"
     gene            3215..4618
                     /locus_tag="PK849_06420"
     CDS             3215..4618
                     /locus_tag="PK849_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPE65798.1"
                     /translation="MEKNLNNSHAMGTDSITRLMWRFSLPAIVGMLANALYNIVDRLF
                     VGQTVGAAGIGAISVVFPFMILVISFDLLVGVGAGSLISISLGEKRRARAERAMGNAI
                     MLLVGGGILLVLLGVTFTESILELSGASATLMPLCLEYMNIAVWGVFFSTLAFGLNFF
                     IRAEGNPRYAMFTLIIGAGANVLLDALFILVFDMGVGGAALATLFSQMISALWAALYY
                     IRRMGTLRFRMKNLAPDWVITRRILAIGSAPALTEVSFTYVLALFNRLLSFYGGDLAI
                     SAMGIFFSLDSLFFLPVLGLAGGVQPIISYNYGAKDYDRVIRSVLAAIKVGALFFTVS
                     FTLVMLFPAPLIRIFNSDDPELLALGVRGMRLAYSGVIFASVSLIASHTFQAIGKAKT
                     GLFLTLSRHFIFILAPLYFLPPILGMDGIWLSLPLSDLGGALTGTWFLRREFRIMRNA
                     ERLQQISAPGECSVSPD"
     gene            4669..>5344
                     /locus_tag="PK849_06425"
     CDS             4669..>5344
                     /locus_tag="PK849_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE65799.1"
                     /translation="MRASVHPFPEGVCEVSTEEGAALFLIDLPIALPGFDHHLSSWLL
                     RDFKRGRNILIDTGPSASVPFLERSLRSLGVERLDLVLLTHIHADHAGGAGRLLERFP
                     EARVLAPERGRTHLVHPSRLAEGTRATLGAAMADAFGPILPVQKERLIDSSDPLLEGF
                     TVLETPGHASHHSSYVFHADFGKILFPGEAAGVGLGEAVRSLWTAPGMPNVSAPYRFP
                     ATPPRLD"
BASE COUNT          951 a         1641 c         1601 g         1151 t
ORIGIN      
        1 cgggaagaac cagctccgga ttatggcaga agggacaccg gaactgacag ccgcaaaaga
       61 agaccacggc cgccgtatta ccggggtagt ccagaaaact tgtaggtatg tagccgccaa
      121 tatgcagttg catcgtgcgc gtccctccgt ggtaaaaaat gtcttctcgg tgaaatagcc
      181 gtgtccctcg aagaaaactc ctctataata ctccttgagg ttcagtgatg tgtgtccctg
      241 atcggaaccc tgaatgtccg tgtatacggt aacgcgcatt tgcgcaggag gttgctcatg
      301 tttctgtttc gactgcttct gaaggcggct ccgttgctgc tcttctttct cttttccaaa
      361 atactgaagg atatgctccg cggggcgcaa ggctcgccgg ggccgtccgg gggcgcgagg
      421 ggcaggtctt cctctggagg aggcgccgcg tatacgcccc ctcccggcgg gaagcgcgac
      481 ccctataccg tgctcggctg ttcgccttcc tcctccaacg aggagatcaa gaagcgctac
      541 cgggagctgc tcgcgaagta ccaccccgac aagttcatcg ggcagaatct cgacgaggac
      601 tttgttgccc tcgcctcaag gaagtttcag gagattcagg aggcgtacgg ccggattcgc
      661 tccgcacggg ggatatagtt tttatctttc ttttcttcgg agcgcggaac ccgacggcat
      721 ctttcttgcg cgactgcggc gggttcttcg gagcgcggcg tgcgtggcga cggtttcaca
      781 ccgcggtatg gagtgtgata caatgacgcc gccagagctc ccctttcgag gggaaaaacg
      841 ttcccgcatg cgggagaaag aggcgaatct ccatgtcaac tctggaactt tttaccggac
      901 ccgagagctt ctctcgtatc aagtcacagg cgcggacgac tattgaatcc gcttttttcg
      961 gcaacaacgt ggttcctgtc tcgaacctca aggaggccta cgagctggcg agagtctccc
     1021 ccggcacggt cgagcttacc gggatgccgg tctaccgtcc cgaagggcag ggattgcccc
     1081 cgcgcagcaa tgtgctcctg atgaacgacg gcgctgtggt cggccgttgc gccgccgcgc
     1141 ggaggatcgt cggcgaaccc ggcgtaaata tgggagattt tgcagcggtg ctccgggaag
     1201 cggtatacaa ctcccgtttc cgtacctttt actccgccga ggcgtatatc ggtctcgaag
     1261 gtgatttcat ggtgaaggcg catcttctcg cgccgaagaa tcatgaaaac ctcgtgtaca
     1321 attggctgct gaacttccag tatctcaaca ggacctatct cgacatgtat gcggcatcca
     1381 ggccgctgcc ggacggcgat atcttcatgt tcgccgatcc cgactggacc tctcccgact
     1441 accccttggg gctggcattc ttcgatccgc tccataactg cgcggcggtg ctgggtatgc
     1501 gctacttcgg cgagctcaag aagggaacgc tgacgctcgc gtggggaaca gccgtgcgga
     1561 acggcttcgc ctcatgccac gggggcctga agcggtacaa cctgaagggg ggacgctcgt
     1621 tcgtcgccgc ggtcttcggc ctctccggtt ccgggaagtc gacgatcacc catgcgcggc
     1681 acgggaacaa gtacgatatc accgtccttc acgacgacgc cttcgtgatc aacgtcaagg
     1741 agaagtacgc catcgcgctc gagccctcct acttcgacaa ggtgcaggac tacccgatgg
     1801 ggtgcgagga caacaagttc atcatcacgc tgcagaactg cggcgttacc cgcggggagg
     1861 acggcctgct ctacgcggtg accgaggacc tgcgcaacgg caacgggcgc gcaatcaagt
     1921 caaagctctg gtcgcccgag cgggtggacc ggatcgacga gccgatcgac gccgttttct
     1981 ggctgatgaa ggactcgtcg ctgccgccgg tgctccgcct gaaggggtcc gacctggccg
     2041 cggtgctcgg cgccaccctc gcaacaaaac ggacgacggc cgagcgactc gcccccggag
     2101 tggacccgaa tgcgctcgtc atcgaatcct acgcaaatcc gttccgtacg tatccgcttg
     2161 ccacggacta tgagcgcttc aaggcgctct tcgacgacgg ggtggcgtgc tacatcctga
     2221 atacaggcga gttcatgggg aagaaagtca agcccgccga cacgctcggc atcctcgagg
     2281 cgcttgtcga ggggaaggcg gagttcgtgc cgttcggccc cttcagcgat atggagacga
     2341 tggcgctgga tcgcttcccg atcgacttca cggatgagca gtaccgaagg gagctcttct
     2401 cgcggatgag ggacaggctt gcgttcgtca cgtcccgaga gacggagaag ggcggtatgg
     2461 accgcctgcc acccgaagcg gtggccgcgc tgcggaaggc gctcgacgag atggggtatc
     2521 ccgtccagga gtaacgtttc ggagccgggg cccgtccccg gctcatcgtt ttctgtatga
     2581 gggagaatag tactcggtcg tcacctcctc gccgcgaaga agccgcgcgc gggagatgat
     2641 cctccgtctg cagacccatt cgaatccttc acgtgcgctc cgttcgtccg tcctgtcctt
     2701 ctccatcccc tcggggtctt tcgccgctcc cggccacgac cgccactccc ccaggagagg
     2761 ctcctggtag agcaccagcc atgaaaaatc attccagaga ccaaggattc tgaagcgctt
     2821 tcgcccctct ccgtcgacgt ggatgagaat actcagctcg tcgcggaagc gcgtggccgt
     2881 tcccgagtac tttttgtccg cgctcttccc ggagaaggac gccgtctgtc cgttgcggac
     2941 gaccccctgc gaggcgcacc attcgcgcag cgttgccatc agcgcgtcga agtccagagg
     3001 aatgccgttc gacgcggcga atgcctgcgc gaatttccgt tcgagcagat attcgccccg
     3061 ccggtgcgag ggggcgcaga gcagatggag acgcagcggg aaaagaaacg cttccgtcat
     3121 gaatcctccc cggagatatt ctcgcccctt tattgcgggg cgctctctgc tataatcata
     3181 cctcaaacaa atttgtacgc ggaggaaggc tcttatggaa aaaaatctga acaactcgca
     3241 cgcgatgggg actgattcga tcactcgtct catgtggcga ttttcgcttc ccgccatcgt
     3301 cggtatgctc gccaacgcgc tctacaacat cgtcgaccgg ctcttcgtcg gacagacggt
     3361 gggcgccgcg ggcatcggcg cgatcagcgt ggtcttcccc tttatgatac ttgtgatctc
     3421 cttcgatctg ctggtgggcg tcggcgcagg ctcgctcatc tccatctccc tcggcgagaa
     3481 gcggcgtgcc agagcggaac gggcgatggg gaacgcgatc atgctccttg taggcggcgg
     3541 aatccttctg gttcttttgg gcgtcacgtt cacagagtct attctcgaac tttcgggcgc
     3601 cagcgctacc ttgatgcccc tctgtctgga gtacatgaac attgccgtat ggggcgtctt
     3661 cttctccacg cttgccttcg ggctcaactt cttcatcagg gccgagggaa acccgcgcta
     3721 cgccatgttc acgctcatca tcggcgcggg agcgaacgtc cttctcgacg cgctcttcat
     3781 cctggtattc gacatgggcg tcggaggggc cgctctcgcg acgctgtttt cacagatgat
     3841 ctccgccctg tgggctgcgc tgtactacat ccgcagaatg gggacgctcc ggttcaggat
     3901 gaagaacctt gcccccgact gggtgatcac ccgcaggatt cttgcaatcg gttcggcgcc
     3961 tgcgctcacc gaggtcagtt ttacgtacgt gctcgccctc ttcaaccggc tcctgagctt
     4021 ttacggaggc gatctggcaa tctcggcgat gggcatcttc ttcagccttg acagtctctt
     4081 ttttctgccg gtcctcggcc ttgcgggcgg cgtccagccg atcatcagct acaactacgg
     4141 cgcaaaggac tacgaccggg tcatccgctc cgtcctggct gcgatcaagg tcggggccct
     4201 cttcttcacg gtgagcttca cgctcgtcat gctctttccc gcgccgctca tccgcatttt
     4261 caactcggac gaccccgaac ttctggccct tggcgtgcgc ggaatgcgac ttgcctacag
     4321 cggcgtcatc tttgcctcgg tgagcctcat agcgtcgcat acctttcagg cgatcggcaa
     4381 ggcgaagacg gggctctttc tgacgctctc aaggcacttt atcttcattc tcgccccgct
     4441 gtactttctg ccgccgattc tcggaatgga cggcatctgg ctgtcgctgc cgctctccga
     4501 ccttggaggc gcgctcaccg gaacgtggtt cctgcggcgg gagttccgta tcatgaggaa
     4561 tgcagaacgt ctgcagcaga tttccgctcc cggcgagtgc agcgtctccc cggattgagt
     4621 ataataccgg tggagcttta tttcacaaat gagtgaggag tgaagggagt gcgcgcatcc
     4681 gtacatccgt ttccagaagg ggtttgcgag gtctctacgg aggagggggc cgccctcttt
     4741 cttatagacc tgccgattgc tcttccggga tttgatcatc atctgtcgtc gtggctgctt
     4801 cgtgatttca agagagggcg caacatcctc atcgacactg ggccgtcggc ttcggtccct
     4861 ttcttggagc gttctttgcg ctctctcggt gtggaacgac tcgatctcgt gctgctaacc
     4921 catattcatg cggatcacgc gggaggggcg ggacgtctcc ttgagcgctt cccggaggcg
     4981 agggtgctgg cgcccgagag gggaagaacg cacctcgtcc acccctcgag gcttgccgag
     5041 ggaacgcgcg cgacgctggg cgcggcgatg gcggacgcct tcggtccgat tcttcccgtt
     5101 cagaaggagc gcctgatcga ctcctctgac cctttgttgg aagggtttac ggtgctcgag
     5161 actccggggc acgcgtcgca ccactcgtcg tacgtcttcc acgcggattt cgggaagatc
     5221 ctctttcccg gggaggcggc gggcgtgggg cttggcgagg cggtccgctc cctctggacg
     5281 gcgcccggca tgccgaatgt ctccgcgccc tatcgttttc ccgcaacccc gccgcgcctg
     5341 gacg
//
LOCUS       DAOPKU010000164         5338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16668_length_5338_cov_5.591520, whole genome shotgun sequence.
ACCESSION   DAOPKU010000164 DAOPKU010000000
VERSION     DAOPKU010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16668_length_5338_cov_5.591520"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1281)
                     /locus_tag="PK849_06430"
     CDS             complement(91..1281)
                     /locus_tag="PK849_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163919.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HPE65800.1"
                     /translation="MSTERIASGDGVVFTDVCKIYPDGTRAVDMLNLDIRRGELVSLI
                     GPSGCGKTTTLKMINRLEEPTSGSIVVGGEDVRSSDAVALRRRIGYVVQEIALFPHLT
                     VAENINVVPELLGWSAAKMRSRADELLEMAGLAPERYRYRLPDQLSGGQKQRIGVLRA
                     LAADPQVILMDEPFGALDPISRETLQNELVSLQKNLRKTIVFVTHDMDEAIRISDRIA
                     ILRKGRVEQIGTPEEIQTAPGNDFIRNFIGEDRLSQMSPDDSVGMLLEEPWAVAAPSE
                     SASAVLERMEENDREFIHVVDPKRGVWLGAALLGRVRRAAVAGKSVVESLSRDRKLYL
                     EDAVLRDVAEMLADRDISIPVLDSGERLLGVITSAGLARLAMNRLRGNRTARAAGEKK
                     EGTR"
     gene            complement(1278..2393)
                     /gene="hisC"
                     /locus_tag="PK849_06435"
     CDS             complement(1278..2393)
                     /gene="hisC"
                     /locus_tag="PK849_06435"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPE65801.1"
                     /translation="MIAGVRKAVGAATPYVPGKTVEEVRRELGLEKIVKLGSNENPYG
                     PFPAARRAMTEEIGRLNTYPDVSFREIKSLIGERFGLGPEWVAISHGAEGMLQTLGKV
                     FLEPGDEAILPCATYGLYREISLVMGATLKEIMLKDDLSVNLREMERAITGRTKLVWL
                     NNPNNPTGMLLPKKSVETLIKRLPRGVWLVLDEAYAEFASPEELPSVAKLLEAECSLI
                     VVRTFSKAWGLAGARIGYALARPEMVRIIDTVSEPFNANRIAIAGASASLKDDEPAFN
                     QALAATVRERERVTKALRDMGGETLPSHTNFVFVTFQTDAGEISEKLLRKGVIVRPCG
                     GWGFGCSLRVTIGTPQQNDFFLEALRGVLSGDGEGGA"
     gene            complement(2393..3988)
                     /locus_tag="PK849_06440"
     CDS             complement(2393..3988)
                     /locus_tag="PK849_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPE65802.1"
                     /translation="MVGNTGGNIAVRVLERLNISVLFGIPGIHNLAVYDGLAHSSIRH
                     IATRHEQGAGFMAYGWGKSTGDPGTALVITGPGLTNILTPMGQAFHDSVPMVVISTQI
                     PSRFLSARSGYLHELRDSTTMSSSVTKGSFRASSPSEIAPLLDRAYRLASAGRPGPVH
                     VEIPLDYLAEPCAPSGAFSSSEQPFFIPDEEFTEALRALGRAQFPVILAGGGASRSAV
                     AVRGLAEALNAPVLLTCAGKGILSEEHPLCLGARLHFPAVRELLAKADCLLALGTEIS
                     PTDLWLDEFEFSGTVVSVDNDEGHRASRNGVFLHGRCEEVLPLLLAGVAKKGAARWAE
                     KDALLARCSQELGATLGVADELPLLAETTRALRTSLGDDGTLWADMTGPAYYAISEYA
                     AALPGRFLHPVGFGTLGAALPAALATKCAFPERRVAALAGDGGFQFTLPELAVGVQEK
                     LSLPVILWNDGGFGEIRRSQQAKGGPLIAVDHWNPDFRALAASYGIPYFAPEEGALGD
                     ALEEAFSAPVPSLIEIRARKECC"
     gene            complement(4295..4591)
                     /locus_tag="PK849_06445"
     CDS             complement(4295..4591)
                     /locus_tag="PK849_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPE65803.1"
                     /translation="MTTENAVTVSGTLHLIKSDPSSPGKQKKYLHFSVRQDTQKTDGS
                     VRKDFLLARVYDPKLQESFKELEEGAKVLVRGDVRSSLGSGEMYINVQEIEKLA"
     gene            complement(4754..>5338)
                     /locus_tag="PK849_06450"
     CDS             complement(4754..>5338)
                     /locus_tag="PK849_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPE65804.1"
                     /translation="GPCHDPRAAKTLGVNYGTGNRGMCHIHPLETVAYDASKFDFGLR
                     PFGLQDPEELPRWEERGKGTAIRILQDAGALPEILGTCKFYMYVGITPREYAEMLSAL
                     TGWNVTGEELMETAERATNLQRLFNIREGFSREDDMLPGRVRSAPAFGTYAGNAECAV
                     RDFDGMLDDYYEARGWDRATGAPTRETLVRLGLA"
BASE COUNT          999 a         1748 c         1604 g          987 t
ORIGIN      
        1 agatgagcag gtgctctccg gtgagccaga gtatccgctg ccagttcctc ccgacgaagg
       61 tgatgaacag gtcgatcagg ctcttttggc tcatcgcgtt ccctccttct tctctccggc
      121 tgcacgggcc gttctgtttc cgcgaagacg gttcatggca agacgggcga gcccggccga
      181 tgtaatgacg cccaggagcc gctccccgga atcgaggacg ggaatggaga tatccctgtc
      241 ggcgagcatc tccgcaacgt cgcgcaacac cgcgtcctcc aagtagagct tcctgtcgcg
      301 ggagaggctt tcgacaacgc tcttgcccgc gaccgcggct cggcgcaccc gcccgagcag
      361 cgcggcgccg agccagacgc cgcgcttcgg atcgacgacg tggatgaact cacggtcgtt
      421 ctcctccatc cgctcgagca cggccgaagc gctttccgac ggcgcagcga cagcccacgg
      481 ctcttcaaga agcatcccga cggagtcgtc gggcgacatc tgagagagcc tgtcctcgcc
      541 gatgaagttc cgtatgaagt cgttcccggg cgccgtctga atctcctcgg gcgttcctat
      601 ctgctcgact ctccctttcc gcagaatggc gatgcggtcg gagatacgga tcgcctcgtc
      661 catgtcgtgg gtgacgaaga cgatggtctt ccggagattt ttctgcagcg ataccagctc
      721 gttctgcagc gtctcgcggc tgatcggatc gagcgctccg aacggctcgt ccatcaggat
      781 gacctgagga tcggccgcca gcgcgcggag aacgccgatg cgctgcttct gcccgccgct
      841 cagctggtcg gggagacggt agcggtagcg ttcgggcgca agtccggcca tctcaagcag
      901 ttcgtccgcc ctgctccgca ttttcgccgc agaccatccg agcagctcgg gaacgacgtt
      961 gatattctcc gccacggtga ggtgcggaaa gagcgcgatc tcctggacca cgtacccgat
     1021 acggcgacgg agcgccacag catcgctcga ccggacatcc tccccgccga cgacgatgct
     1081 cccggacgtc ggttcttcga ggcggttgat catcttaagc gtcgtcgtct tgccgcaccc
     1141 cgacggaccg atgaggctga ccagctcccc cctgcgaatg tcgaggttca gcatgtcgac
     1201 cgccctcgtt ccgtcagggt agatcttgca gacatccgtg aagacgacgc cgtctcctga
     1261 agctattcgc tccgtgctca tgctcctccc tccccgtctc ccgaaagaac gccgcgaagc
     1321 gcctcaagga agaagtcgtt ctgttgcggc gtgccgatcg tcacgcggag cgaacaaccg
     1381 aacccccagc cgccgcaggg gcgcacgatg acgcccttgc ggagcagttt ttccgagatc
     1441 tcgcccgcgt ccgtctggaa ggtgacgaag acaaagttgg tgtgcgaagg aagcgtttcg
     1501 cctcccatat cgcgaagcgc cttggttacc cgctcccgct cccgcaccgt cgccgcgagc
     1561 gcctggttga acgcgggttc gtcatccttc agcgacgccg aggcgccggc gatggcgatc
     1621 ctgttggcgt tgaacggctc gctcaccgtg tcgatgatcc gcaccatctc cggacgcgcc
     1681 agcgcatacc cgatacgcgc cccggcgagt ccccacgcct ttgaaaaagt ccgcacgacg
     1741 atcaggctgc actctgcctc aagcagcttc gcgaccgaag gcagctcttc cggggaggca
     1801 aactcggcgt acgcttcgtc gaggacgagc cagaccccgc ggggaaggcg ctttatcagc
     1861 gtctcgacgc tctttttggg gagcagcatc cctgtggggt tgttcgggtt gttcagccac
     1921 accagcttgg tgcgcccggt gatcgcgcgt tccatctccc gcaggttgac ggagagatcg
     1981 tccttgagca tgatctcttt cagcgtcgcc cccataacga gcgagatctc ccggtagagt
     2041 ccgtaagtgg cgcacggaag aatagcctcg tcgccgggct ccagaaagac tttgccgagc
     2101 gtctggagca ttccctcggc gccgtgcgag atcgccaccc actccgggcc gagcccgaag
     2161 cgctcgccga tgagactctt gatttcgcgg aaggagacgt ccgggtaggt gttgagccgc
     2221 cctatctcct ccgtcattgc cctgcgggct gcggggaagg ggccgtacgg gttctcgttc
     2281 gagcccagct tcacgatctt ttcgagcccc agctctctgc ggacctcttc gacggtcttc
     2341 cccggaacat acggggtcgc cgctccaacg gccttgcgaa ctcctgcaat catcagcaac
     2401 actcctttcg ggcgcggatt tcgatcagcg acgggacggg ggcggaaaac gcctcttcga
     2461 gcgcgtcgcc gagcgcgcct tcttcaggcg cgaaataggg gattccatag gaggccgcca
     2521 gcgcacggaa gtctgggttc cagtgatcga cggcgatgag cggaccgcct ttcgcctgct
     2581 gggaccgccg gatctcgccg aagccgccgt cgttccacaa gatcaccggg agcgacagtt
     2641 tttcctggac acccaccgcg agctccggaa gcgtgaactg aaaacctccg tcgcctgcga
     2701 gcgcggcgac gcggcgttcc ggaaaggcgc acttcgtcgc caacgcggcg ggcaacgccg
     2761 cgccgagcgt cccgaagccg accgggtgga ggaaacgccc ggggagcgcg gcggcgtact
     2821 cgctgatcgc gtagtacgcc gggcccgtca tatccgccca gagcgttccg tcgtcgccga
     2881 gcgaggtcct gagcgccctc gtcgtctctg cgagcagcgg caactcgtcc gcgacgccaa
     2941 gcgttgcgcc cagctcctgc gagcagcggg caagaagcgc gtccttctcc gcccaccgcg
     3001 cggcgccctt tttggcgact ccggccagca gcagcggaag cacctcctcg caccttccgt
     3061 gcaggaacac tccgttgcgg gaggcgcgat gcccctcgtc attatccaca gagaccacag
     3121 ttccggagaa ctcgaactca tccagccaaa gatcggtcgg ggagatctcg gtaccaagcg
     3181 cgaggagaca gtcggccttc gcaagcagct ccctgaccgc gggaaagtga aggcgcgcgc
     3241 cgaggcagag cggatgctct tcggagagaa tgcccttccc cgcgcacgtg agcagaaccg
     3301 gtgcgttcag cgcctcggcc agtccgcgga ccgcaacggc ggagcgggac gccccgcccc
     3361 ccgcgaggat cacggggaat tgagctcgcc cgagcgcccg gagagcctcg gtaaactcct
     3421 cgtcggggat gaaaaacggc tgttcgctcg aagaaaacgc ccccgaaggc gcgcacggtt
     3481 cggcgaggta gtcgagcgga atctccacgt ggacgggccc gggcctgcct gcggaggcca
     3541 accggtacgc acgatcaagg agcggcgcga tctccgaggg agaggaggcg cgaaagctgc
     3601 ctttcgtcac ggaagaggac attgtcgtgg aatcacgcaa ctcgtgaaga tatcctgaac
     3661 gggctgagag aaaacgcgag gggatctgcg tggagatgac gaccatcgga accgaatcgt
     3721 ggaacgcctg ccccatgggg gtgaggatgt tggtgagccc cggccccgtg atgacgagcg
     3781 ccgttccggg gtcgccggtg gactttcccc atccgtacgc catgaacccg gccccttgtt
     3841 cgtgccgcgt tgcgatatgc cggatggagc tgtgcgcgag accgtcgtag acggccaggt
     3901 tatgaatgcc cggtatgccg aagagaactg aaatgttcaa acgctccagg acccggacgg
     3961 caatattccc gccggtattc cctaccattg aatcgacctc ctgaatgccc aattttaagg
     4021 ggaaaacatc tgaaaggggt tccccttgcg ctgtgcgcaa gagtacaccc cgtacatctt
     4081 ctatgcagta cgcatggcgg cagagtggtc gggcttgggg aaaaaaacaa catagcgcgg
     4141 aagactccgt gcgatgaaag ggagaaacgc cacttttttc ggcacacaac cgacaaaggg
     4201 tatcgacagt cgaaaaatcg acaccaagcc ctgtaatgaa ggaaacgcat gagaaacgtc
     4261 aggagaagac gttcctcggt tcatacgaaa aactctacgc cagcttttct atctcctgga
     4321 cgttgatata catctcgccg ctgccgagag aggaacgcac atctccgcga accagcactt
     4381 tcgcgccctc ctcaagctcc ttgaaggatt cctgaagctt cgggtcatag actctggcga
     4441 gaagaaaatc ctttcggacg cttccatccg tcttttgcgt atcctgacgc actgagaaat
     4501 gaagatattt cttctgcttg ccggggctgg acggatcgct tttgatcaga tggagcgtcc
     4561 ctgaaaccgt cacggcattt tccgtcgtca tcggacatca actcccgttc tcggaatgta
     4621 cggggtctct tgcgagcgcc ccgtccttca aattgttacc atattgtacc atccttgccc
     4681 ctctttgtcg ccttgtttcc gaaacgaaat atacgatgta ccctatattt caaggagcgc
     4741 gcgcagtatc ctgctacgcc agaccgagac ggacaagcgt ctcccgcgtc ggcgcgccgg
     4801 tcgccctgtc ccacccccgg gcttcgtagt agtcgtcgag catgccgtcg aagtcgcgca
     4861 ccgcacactc cgcgtttccc gcgtacgtcc cgaacgcggg cgccgaccgc acccttccgg
     4921 ggagcatgtc gtcctccctg gaaaagccct cccggatgtt gaaaaggcgc tggagattcg
     4981 tcgcgcgttc cgccgtctcc ataagttcct ctcccgtgac gttccatccg gtaagcgccg
     5041 aaagcatctc cgcgtactcc cgcggggtga tgccgacgta catgtagaac ttgcaggtcc
     5101 ccagaatctc cggcagcgcc cctgcatcct ggaggatccg aatcgccgtt ccctttcccc
     5161 gctcctccca gcggggcaac tcctccgggt cctggaggcc gaagggacgc aggccgaaat
     5221 cgaacttcga cgcgtcgtag gccactgttt caagggggtg gatatggcac atcccgcggt
     5281 tgccggtccc gtagttcacg ccgagcgttt tcgccgccct cgggtcgtgg cacggacc
//
LOCUS       DAOPKU010000165         5336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16676_length_5336_cov_4.226472, whole genome shotgun sequence.
ACCESSION   DAOPKU010000165 DAOPKU010000000
VERSION     DAOPKU010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16676_length_5336_cov_4.226472"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PK849_06455"
     CDS             complement(<1..1072)
                     /locus_tag="PK849_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPE65805.1"
                     /translation="MSSIWGKNFSNIARNNRPSPIREMLAVIKQPGMISFAGGMPAPE
                     IFPIDQFYEGAHILKDDGANLLQYGTTEGYPPLKEFLASWTAPRMGRTVGLDEMLITT
                     GSQQALDLFAWSMIDEGDYVVVEDPSYLAALTVFFNHGGRFLGVPMDEEGMQVDLLPG
                     IIEKARSEGKRIKFIYTIVNFQNPGGSTMSVPRRKQLVEISKKYNIPVFEDDPYGYVR
                     YDGEHLPSIFSFHDEEGVVYAGSFSKILSPGTRVGWIAGPKEIIRNLTVFKQGTDLCS
                     SPITQALVAEYCAKGYLDSHLPVIIDNYRVKRDAMEKSFEKHLAPLGVTWVKPEGGFF
                     YWLNVPSVNTNDLFKKAIEKKVA"
     gene            complement(1416..2045)
                     /locus_tag="PK849_06460"
     CDS             complement(1416..2045)
                     /locus_tag="PK849_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPE65806.1"
                     /translation="MKRVIGRLALLIVLLLAFSAVSEGALDRATVDRAWTRIAAHAGI
                     PWQQVIYEEQQEPNAWVKFSPGNHTVHVTTGLMAILTKEDEIAGILAHEAGHIQLGHY
                     SSSVSRNLLWGLLFNSLKGDAAREIVGGVGMVLAESGFSREQEVEADDYGIRVSSAAG
                     YSPWGLVRAMEKMKAAGFKTSPSGFNSHPPTERRLLRLRNNAAAIAPRN"
     gene            2199..3329
                     /locus_tag="PK849_06465"
     CDS             2199..3329
                     /locus_tag="PK849_06465"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPE65807.1"
                     /translation="MLHCVQCGKTFDEASLLWRCPCGGVLDWTVPEPFKRSSIRTEET
                     GMWRYAASLPLDSPDSRVSTGEGGTPLIPGDWNGLSVFWKLDFLCPTGSYKDRGMACL
                     VNRLRELGVRELIEDSSGNAGSSMAAYCALAGIGCKIYVPGYTSEGKCVQILSYGGEL
                     VRIPGTREDTSRAAEKAASETYYASHNWSPYFAHGVKTFAFEVWEQLGFQLPDAVLLP
                     AGQGSLVLGWAQAFEELRASGEIDRLPRIYALQPENCAPLALAFEKRTDAPEPFPKKE
                     TIAEGISSAEPVRGVQVLSAVRRSGGAVRAFSERAIWDGFNKLARKGLYVEPTSAIVG
                     AALDELRNAGELREGERLVALLSGSGLKATDKILSLIGNPPA"
     gene            3383..4621
                     /locus_tag="PK849_06470"
     CDS             3383..4621
                     /locus_tag="PK849_06470"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPE65808.1"
                     /translation="MGRVTVNNLQEGMVLEEDLRAPNGRFILGKGVTLKEKYIRMFKI
                     WGITGADVEGVEDGEVVRRALLEEDAIRRSRSYVDTYFPPEDAEDVCASAVRSFCVRR
                     FAEEFASGTPPSPLPEVKSLPRPPEGHFGRSFSAYALAKDEVRLFSFPDIYFKIQEVI
                     NSPISSATSIAKVVGNDPNLAARLLKLVNSSFYGFPNPIQSIPRAIAIIGSNELTSLA
                     LAVSTMKVFKNVPKEYVDMKSFWRHSIACGVFSRLLAYNKRLRSEERFFLAGLLHDLG
                     RILLYTKAPNEMTYALELSLFEHIPLWRAEKRVFGFDHTAVGKTLMEEWRIPRGISLL
                     CDSHHNPTSEEAPEEAVFVHLADYLANSLRIGTSGSLYFSGVDRALWELLELDEGDVE
                     AVLVQGERQIREIMNIFLMT"
     gene            4932..>5336
                     /locus_tag="PK849_06475"
     CDS             4932..>5336
                     /locus_tag="PK849_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65809.1"
                     /translation="MDDGTFAWAVDRNKPVIITARPGKTSAGLEVKERQLLLHSIMSS
                     NRSIGMFMSLLGEDETDILDVSFAFFTVLLGSMASILHNAELYTMIQGLNNELQGKIK
                     RLEESERSLAEAMKAKEVFLANVSHEVRTPLNG"
BASE COUNT         1218 a         1338 c         1468 g         1312 t
ORIGIN      
        1 aggcgacttt tttctcaatg gcttttttaa aaaggtcatt tgtatttact gaaggcacgt
       61 tcagccaata gaaaaagcct ccctcgggtt tgacccaggt aacgcccaaa ggagcgaggt
      121 gtttttcaaa gcttttctcc atagcgtcgc gcttgacccg gtaattgtca atgataacgg
      181 gcagatggct gtcaagatag cctttggcgc agtattcggc gacgagcgcc tgtgtaatgg
      241 ggctggagca gaggtccgtc ccctgcttga aaacggtcag gttgcgaatg atctctttgg
      301 ggccggcgat ccagcctacg cgagtgccgg gcgagagtat cttcgagaag gaaccggcat
      361 agacaacgcc ctcctcgtcg tggaatgaga agattgaagg gagatgctcg ccgtcataac
      421 gaacgtaccc gtaagggtca tcctcgaaga cagggatatt gtattttttt gaaatctcca
      481 cgagctgttt gcgccgggga acggacattg tcgaaccgcc cggattttgg aaattgacga
      541 tggtgtagat gaattttatt cttttcccct cggagcgtgc tttttcgatg attcccggaa
      601 ggagatcgac ctgcattccc tcttcgtcca tcggaacgcc gaggaacctg ccgccgtgat
      661 taaagaagac agtgagggca gccaggtatg aggggtcttc gacgacgacg tagtcgccct
      721 cgtcgatcat ggaccatgcg aaaagatcga gggcctgttg agatcctgta gtaataagca
      781 tttcatcgag accgacagtg cgtcccatcc ggggggcggt ccatgaagcc aggaattcct
      841 tgagcggagg gtacccttcg gtggtgccgt actgaaggag gtttgcgccg tcatccttca
      901 gaatgtgcgc tccctcgtaa aactgatcga tagggaagat ttcgggcgca ggcatccccc
      961 cggcaaaaga gatcataccg ggctgcttga taacggcgag catctcgcga attggcgaag
     1021 gacggttgtt cctcgcgatg ttgctgaagt tctttcccca gatactgctc atacatatcg
     1081 cccccttgat gatatggtaa atttgagtct ttctgagcgt aagctccaaa aaaaactcga
     1141 aaagagagat aatacaaatc aattatagca cgaagaagaa aaggaaaaaa ggaagaaaaa
     1201 aagagagatc ggatgtttgg cgtgtgaacg cagcaaaaac agtcctgacg caacactctg
     1261 tatttcggtc tggttcccgg ccgtttgtag tcgatgccga acgagagacc ggatgaggac
     1321 atgtcaaaag gtgtgaagga gctccggtgg taaaacaaca aaaaagaact acacccggag
     1381 gaatcatcct ctcctccggg tgtttaagaa atgtgtcagt tccggggggc tattgccgcc
     1441 gcgttgttcc tcagacggag cagtctgcgc tccgtgggag ggtgggagtt gaagccgctc
     1501 ggggaggtct tgaatccagc ggctttcatc ttttccatgg cgcgtaccaa cccccagggg
     1561 ctgtatccgg ctgccgacga tacccgaata ccgtagtcgt ctgcttcaac ctcttgttcc
     1621 ctgctgaatc cggattcggc aagaaccatt ccgaccccgc cgacaatctc cctcgcggca
     1681 tcccctttca gggaattgaa cagaagcccc cagagaagat tgcggcttac ggagcttgaa
     1741 tagtggccga gctggatgtg tcccgcttca tgagcaagta tgcccgctat ttcgtcttct
     1801 tttgtcagta tcgccatcag gccggtcgtc acatgaacag tgtgattgcc gggcgaaaat
     1861 ttaacccagg cgttcggttc ttgctgctct tcataaatga cctgctgcca tgggataccg
     1921 gcatgagcgg caatacgcgt ccacgcccgg tctaccgtcg ctctgtcgag agcgccctcg
     1981 gaaacagctg aaaacgccag aagaagtacg ataagaagag cgaggcgtcc gattaccctc
     2041 ttcattcgaa ttcctccttt tctcttttgt gtagttctat tttccaatag gatacatcct
     2101 ttcttctttt agtgaaaggg ggaaacttcg taggagtcaa taggcagtat cggacattct
     2161 gtatataatg aaatcagaat cgagaggagg ttgattgcat gctgcactgt gttcaatgcg
     2221 gaaaaacgtt cgacgaagca tccttattgt ggcgctgtcc atgcggaggt gttctcgact
     2281 ggactgtacc cgagcccttc aaacgctcat cgatacgcac ggaagagacc gggatgtggc
     2341 ggtacgctgc ttctctccct cttgattctc ccgattcgcg tgtttctacg ggggagggag
     2401 ggactcctct tattcctgga gactggaacg ggctttccgt tttctggaag cttgattttc
     2461 tctgccctac aggctcatac aaagaccgcg gaatggcgtg tcttgtcaat cgtttacgcg
     2521 aactcggcgt gcgtgagctc atagaggact cctcaggaaa tgcgggctcg tcgatggcgg
     2581 catactgcgc tcttgccggg atcgggtgta aaatctacgt tccagggtac acgtccgaag
     2641 gcaagtgcgt tcagatactc tcttacggcg gggaacttgt ccgtataccc ggtaccagag
     2701 aggatacttc tcgtgcggcg gagaaagcgg cgagtgaaac ctattatgcc agccataact
     2761 ggagccccta cttcgcccac ggcgtaaaaa cctttgcctt cgaagtatgg gagcagttgg
     2821 gattccagct tccggacgcg gttcttcttc ctgccggtca gggaagtctt gttcttggat
     2881 gggcgcaagc attcgaagaa ctgcgcgcct ctggagagat agaccgtctt ccgagaatct
     2941 acgcgcttca gcccgaaaac tgcgcacctc tggcgctcgc cttcgaaaag cggaccgacg
     3001 ctcccgagcc gttcccaaag aaagagacca tagcggaagg tatcagttct gcggaaccgg
     3061 ttcgcggggt tcaggttctt tccgctgtgc gaagaagcgg aggggctgtc cgtgcctttt
     3121 ctgagagggc tatttgggat ggctttaata agcttgcacg gaaggggctg tacgtggagc
     3181 cgacaagcgc gatcgtcgga gcagcccttg acgagctgcg gaacgcggga gaactccgcg
     3241 aaggagagcg tcttgtcgcg ttgctttccg gttccggact gaaagcgacc gataagattc
     3301 tctcgctcat cggcaatcct ccggcctgat acttcttctt gctgtggtat agttatccta
     3361 ttttgagccg gggagagaaa atatgggccg tgtgactgtg aataaccttc aggaaggaat
     3421 ggtcctcgag gaggatcttc gcgccccgaa cgggcgtttc atccttggaa aaggggtgac
     3481 cctgaaggaa aaatacatca ggatgttcaa gatttggggg attaccggcg ccgacgtcga
     3541 aggcgtcgaa gacggtgaag tcgtccgaag ggcgctcttg gaagaggacg cgatccggcg
     3601 gtcccgttcc tatgttgata cgtacttccc tcccgaggac gccgaggatg tatgcgcttc
     3661 tgcggtccgt tctttttgcg tgcgccggtt cgccgaagaa tttgcttccg gaacgccgcc
     3721 ttctcctttg ccggaagtga aaagccttcc acgccctcca gagggacatt ttggacgctc
     3781 tttctctgca tacgcgctgg caaaagacga agtgcgtctt ttctcatttc cggacattta
     3841 tttcaagata caggaggtta ttaactctcc gatcagttcg gctacgtcca tagcaaaggt
     3901 ggtcggcaac gatccgaacc ttgccgcccg tctgctcaag cttgtgaaca gctctttcta
     3961 cgggttcccc aacccgatcc agtcgattcc cagagcgatc gcgatcatcg gttccaatga
     4021 actgacctcg cttgcccttg cggtttcgac catgaaggtt ttcaagaacg tcccgaaaga
     4081 gtacgttgac atgaaatctt tttggagaca ttcaatagcc tgcggagtct tttcacgcct
     4141 tttagcatac aacaaacggc ttcgttcgga agagcgcttt tttctcgccg ggctgctgca
     4201 cgatctcggc cggatactcc tttacacaaa ggctccaaat gagatgacct acgcgctcga
     4261 actttcgctt tttgaacaca ttcccctctg gcgtgccgag aagcgagtat tcggcttcga
     4321 tcataccgct gtagggaaga cgctcatgga ggagtggaga attccccggg ggatcagtct
     4381 tctctgtgat tcccatcaca accctacgtc ggaagaagcc cccgaagaag cggtattcgt
     4441 ccatcttgcg gattatctgg caaacagtct gcgtattggc accagcggct ctttatattt
     4501 ctcgggagtg gatcgcgcgt tgtgggagct cctggaactg gatgagggcg acgtggaggc
     4561 tgtgcttgtc caaggagagc gtcagatacg cgagataatg aatatctttt taatgacgta
     4621 atgtaaagat gaaaccttca gacgagagta tagaccagct tcgggagaga gttcgggttc
     4681 ttacgggaga aagagaggcg acaaaacgga ttcttgaggc cgccgtcgac tctcttgctt
     4741 tccccgttgt tgtcgatgaa accttctctt ccgacgtctt attggcccag gctgcgcaga
     4801 aactccgctc tttcatcaag ttagacgctc ttgtcttcta tctctttaca ccggatgggc
     4861 tggacttttt ccccgcgttt tgcgacccgc ccgaaagact ctcctttttc gaggaggaaa
     4921 tgattcctct cgtggatgac ggaacatttg cctgggctgt cgaccggaac aaaccagtca
     4981 taatcaccgc tcgtcccggg aaaacgagtg cgggtcttga agtgaaagaa cgccagcttc
     5041 tgttgcattc aataatgtcg tcaaaccgca gcatcgggat gtttatgagt cttttaggag
     5101 aggacgaaac agatattctt gatgtctctt tcgccttctt tacagttttg cttgggtcta
     5161 tggctagtat ccttcacaat gccgagctgt ataccatgat tcaaggtttg aataatgagt
     5221 tgcaaggtaa aatcaaacgg ctggaagagt cggaacgtag tcttgcagaa gcaatgaagg
     5281 cgaaagaggt ctttcttgcg aatgtcagtc atgaagtgcg tacgccgctc aacggt
//
LOCUS       DAOPKU010000166         5301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16830_length_5301_cov_5.335875, whole genome shotgun sequence.
ACCESSION   DAOPKU010000166 DAOPKU010000000
VERSION     DAOPKU010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16830_length_5301_cov_5.335875"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1455)
                     /locus_tag="PK849_06480"
     CDS             complement(43..1455)
                     /locus_tag="PK849_06480"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HPE65810.1"
                     /translation="MEEQKERGSFIPVRTDDLVRALCEERALSGEQREKFKKVANLVE
                     ALYHYDFHDTLEKARGAYHFFNPDADTLKRYAPDTDPAKKFDAMVKALEKILVAANYR
                     EFPLDELNALMTKAAPEGLDVQVDLARYEKILIYRRGSKLAPKARNLLSRTKFFRKKD
                     APAEPETEVSPPTHDQEEIIQRLLILIKLKSSEDMEQFYDSFIETAGETGQERARRMA
                     EKALFGKKEPKLHGASSGTPAIFLKVFKNVPVSALETLFPDVTVRMTLLDKGLILLPL
                     VGGILSVVNRVIPALLLIGTIIAALVAGRAIDWGEFRNHLFPILTAFSIVGVITFKIF
                     SKYKITKEKHQAKLMKTLYFHNLDNNAGVFDFLVQEAEEEECKEALLAYYFLLTEKDA
                     NGEPFTEEELDRRIEQWMEERFGMTLNFEVDDALRKLEEKKLLVKDGDRYRVPPIDET
                     LIRHDELWDGVFAYSNERKE"
     gene            1694..4588
                     /locus_tag="PK849_06485"
     CDS             1694..4588
                     /locus_tag="PK849_06485"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HPE65811.1"
                     /translation="MQKQFKGLTSQEVLESRRTHGENVLTPPVRESWWKLYLEKYDDP
                     VIRILLIAACIAFLVGLAHNEYVEAVGIVVAVFLATTMAFVNEYKAGREFDILNQVND
                     DVEFKVIRDGEYRSVSKKELVVGDILLLETGEEVPADAVVEEAVALQVDESTLTGESK
                     AVSKFSEDDPRGAEKREAATYPPHRVYRGTMVQDGHGVFRIESVGDATEIGKTAREAA
                     AVSEERTPLSRQLDGLSKVIGVVGFGIAAVLFAVLSVHAALVGEIVMERGQWIFALIL
                     IVSVLVALVRVWLPIVYDFFDVIRRPKTRPGWLQQEGITAWLKPLLYGAAFFALASGA
                     AWTLGALPSSPSNFLDADAGRRFLQFFMIAITLIVVAVPEGLAMSVTLSLAYSMRKMT
                     ASNNLVRKMHACETIGAATVICTDKTGTLTKNKMHVHEVRVPSLNASPELLYEAMAAN
                     STANLSKSGGETEALGNPTEGALLLWLDERGEDYKALRDSFDVLDQLTFSTERKYMAT
                     FGKGRDGGVVLHAKGAPEILLETCSSILSESGEEALTDGVKEEILSSIGSFQARGMRA
                     LGFAVRRELPEAGEVSAESLMEGLTWLGFTAIADPVRDDVPDAIRACTGAGIGVKIVT
                     GDNRETAREIGRQIGLVGDGPEAEFALLTGDEFETMVDAPETRLRLLDELKVLSRARP
                     SHKLKLVKALQEKGEVVAVTGDGTNDAPALNHANVGLSMGKRGTAVAKEASDIVLLDD
                     SFTSIVNAILWGRALYQNIQRFLLFQLTVNVTALLIVLLGPVVGIELPLTVIQMLWVN
                     LIMDTFAALALATEPPHSEVMKSKPRNPEAFIVTPVMTHGIFGYGVFFVVVLASMLLL
                     IRGGGVSPYELTVFFCVFVMLQIWNLFNARCLGLKTSAFSGLTENRWFAAIVLLVLAG
                     QILMVQFGGKVFRTVPLSLVDWLLIIVGTSPVLLVGELLRFRARRRETAA"
     gene            complement(4769..>5301)
                     /locus_tag="PK849_06490"
     CDS             complement(4769..>5301)
                     /locus_tag="PK849_06490"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HPE65812.1"
                     /translation="LIAAIVAGRNIDWAHFKEQLFPILAAFSIVGTIAFKVFTKYKLT
                     KEKHQAKLMKTLYFHNLDNNAGVFDFLVHEAEEEECKEALLAYYFLLTGKDSEGKAYS
                     QDELDDRIEQWMKEKFGVTLDFEVDDALRKLEDKKLLVKEGDRYLVPSIDDTLVKLDE
                     LWDTIFTYSNEAEGNA"
BASE COUNT          930 a         1548 c         1732 g         1091 t
ORIGIN      
        1 taatctgttt cggcgatcat gaagctaccg ggcttgcttc ttctactctt ttctttcgtt
       61 gctgtacgca aaaacgccgt cccacagctc gtcgtggcgg atcagggttt cgtcgatcgg
      121 cgggactcgg taccggtcgc cgtcctttac gaggagcttc ttctcctcca gcttgcggag
      181 ggcgtcgtcc acctcgaagt tcagcgtcat cccgaagcgt tcttccatcc actgctcgat
      241 gcggcggtcc agctcttctt ccgtgaacgg ctctccgttc gcgtctttct ccgtcagcag
      301 gaagtagtag gcaagcagcg cctccttgca ctcctcctcc tcggcctcct gcacgagaaa
      361 gtcaaagacc ccggcgttgt tgtccaggtt gtggaagtag agcgtcttca tcagctttgc
      421 ctggtgcttc tccttcgtga tcttatactt ggagaagatc ttgaaggtga tcacgccgac
      481 gatggagaac gcagtgagaa tggggaaaag atgattgcgg aactcgcccc agtcgatcgc
      541 gcgcccggcg accagcgcgg cgatgatcgt gccgatgagg agcagcgcgg gaatgacgcg
      601 gttcaccacg ctaaggatgc cgcctaccaa ggggaggagg atcaacccct tgtcgagcag
      661 cgtcatccgg acggtcacat ccgggaagag cgtctccagc gcggagacgg ggacgttctt
      721 gaagaccttc aggaagatgg cgggcgtccc cgaggaagcg ccgtgcagtt tgggctcctt
      781 cttgccgaag agggctttct ccgccatgcg gcgcgcgcgt tcctgtccgg tttcgccggc
      841 ggtttcgatg aaagagtcgt agaactgctc catatcctcg ctgctcttca gcttgatcag
      901 gatcagaagg cgctggatga tctcctcctg gtcatgcgtc ggaggcgaga cctcggtctc
      961 cggttccgca ggcgcgtctt tcttccggaa gaattttgtc cgagacagca agttacgagc
     1021 cttcggggca agtttcgacc cccttcggta gatcaggatt ttctcgtacc tggcaaggtc
     1081 gacctgcaca tccagcccct cgggcgcagc ctttgtcatc agcgcgttga gttcgtcgag
     1141 gggaaactcc ctgtagtttg cggcgacgag gattttttcc agcgccttca ccatcgcatc
     1201 gaacttcttt gcggggtcgg tatccggtgc gtagcgtttc agcgtgtcgg cgtcggggtt
     1261 gaaaaagtga taggctcccc gcgccttctc cagcgtatcg tgaaagtcgt agtggtagag
     1321 cgcctcgacg aggttcgcaa cttttttgaa cttctcccgc tgttccccgg agagcgccct
     1381 ctcttcgcac agagcccgca cgaggtcgtc ggtgcgcacg gggatgaacg agccccgctc
     1441 tttctgttcc tccatcgtaa ctcctcctgt actcggcttt tctgtgcgga cgtcccgctg
     1501 cggggcgcat ccgcgcagaa agtatagcac ggcgttcacg tttcttcagg cgtttcgaga
     1561 agggggtatt tcggtcgcgc attctcttcg gtgacgaaga agcgcgccct gaaagcgtat
     1621 aatggaaccg ttgcgcaaag agacgggaag acggccttca caggtcgtcc gcgtggatat
     1681 ttggaggtgt gctatgcaga aacagttcaa gggtttgacc tctcaggagg ttctggagag
     1741 caggagaacg cacggagaaa acgtgctcac cccgccggtg cgggagtcat ggtggaagct
     1801 gtacctggag aagtacgacg atccggtgat ccggattttg ctcatcgcgg cgtgcatcgc
     1861 cttcctggta gggcttgccc ataacgagta cgtggaggcc gtcggtattg tcgtcgcggt
     1921 gttcctcgcc acgacgatgg cctttgtcaa cgagtacaag gcgggcaggg agttcgacat
     1981 actcaatcag gtgaacgacg acgtcgagtt caaggtgatc cgggacggcg agtaccggag
     2041 cgtttcgaag aaggagcttg tcgtggggga tatcctgctg ctggagacag gggaggaggt
     2101 cccggcggac gccgtcgtcg aggaggccgt cgcgctgcag gtcgacgaat ccacgctgac
     2161 gggagagtcg aaggccgtct ccaagttttc cgaggacgat ccgaggggcg cggaaaaacg
     2221 agaggcggct acctacccgc cgcatcgcgt gtacagggga acgatggtgc aggacgggca
     2281 cggggtcttt cgcattgaga gcgtcggcga cgccacggag atcgggaaga cggccaggga
     2341 ggccgcggcg gtttccgagg agcgcacgcc gctgagccgt caactcgacg gcctgagcaa
     2401 agtgatcggc gtcgtcggct tcggcatcgc ggcggtgctc ttcgccgtat tgagcgtcca
     2461 cgccgcgctc gtgggagaga tcgtcatgga gcgagggcag tggatctttg cgctcatcct
     2521 gattgtctcg gtacttgtcg cgctggttcg tgtgtggctg ccgatagtct acgacttctt
     2581 cgacgtaatc cggcggccga agacgcggcc ggggtggctg cagcaggaag ggattacggc
     2641 gtggctgaag ccgctgctct acggcgcggc gttcttcgcg cttgcatcgg gcgctgcgtg
     2701 gacgcttggc gcgttgccct cgtctccctc gaacttcctg gacgccgacg cagggcggcg
     2761 attcctccag ttcttcatga tcgccattac cttgatcgtg gtcgccgtcc ccgaggggct
     2821 ggcgatgagc gtcacgctct cgctggcgta cagcatgcgg aagatgaccg cctcgaacaa
     2881 cctggtgcgc aagatgcacg cctgcgagac cataggcgcc gcaacggtaa tctgcaccga
     2941 caagaccggc acgctgacga agaacaagat gcacgtgcac gaggtgcgcg ttccctcctt
     3001 gaacgcctct ccggagctgc tctacgaggc gatggcggcg aattcgacgg cgaacctctc
     3061 gaagtcgggc ggggagacgg aagcgctggg gaatcccacg gagggggcgc tgctgctctg
     3121 gctcgacgag cgcggagagg actacaaggc gctgcgggac tcattcgacg tgctggatca
     3181 gctcacattc tccaccgagc ggaagtacat ggcgaccttc ggcaaaggac gcgacggcgg
     3241 cgtggtgctt cacgcgaagg gggcgcccga aatactgctc gaaacgtgct cctcgatcct
     3301 ctccgagtct ggagaagagg cgctgacgga cggggtgaag gaggaaattt tgtcctccat
     3361 aggctccttc caggccaggg ggatgcgcgc cctcggcttc gccgtgcgcc gcgagcttcc
     3421 cgaggccgga gaggtctctg ccgagagcct tatggagggg ttgacatggc tcggattcac
     3481 agctatcgcc gacccggtgc gcgacgacgt acccgacgcc atccgcgcct gcacgggggc
     3541 gggcatcggc gtgaagatcg tcaccggcga caaccgggag acggcgcgcg agatcggccg
     3601 tcagataggg ctggtcggcg acgggccgga ggcggagttc gcgctgctga cgggggacga
     3661 gttcgagacg atggtcgacg ctccggagac acggctgcgg ctgctcgacg aattgaaggt
     3721 gctctcgcgg gcgcgcccct cgcacaaact gaagctggtg aaggcgctcc aggagaaggg
     3781 agaagtcgtc gcggtgacgg gcgacgggac gaacgacgcc cctgcgctga accacgcgaa
     3841 cgtcgggctc tccatgggga agcggggaac cgccgtggcg aaggaggcca gcgacatcgt
     3901 gctgctcgac gactcgttca cgagcatcgt caacgcgatt ctgtggggga gggcgctcta
     3961 ccagaacatc cagcgcttcc tgctcttcca gcttacggtg aacgtgacgg cgctgttgat
     4021 cgtgctgctc ggcccggtgg tcgggataga gcttcccctg acggtgatcc agatgctctg
     4081 ggtcaacctg atcatggaca ccttcgcggc gctcgccctg gcgacggagc cgccgcacag
     4141 cgaggtgatg aagagcaaac cccggaatcc ggaggccttc atcgtgacgc cggtaatgac
     4201 gcacgggatc ttcgggtacg gcgtcttttt cgtggtcgtc ctcgcctcca tgctgctgct
     4261 gatacggggc ggcggcgtct ctccctacga gctgaccgtc ttcttctgcg tctttgtgat
     4321 gctccagata tggaatctct tcaacgcgcg ctgcctcggc ctgaaaacct cggcgttcag
     4381 cggcctgacg gagaaccggt ggttcgcggc catcgtcctg ctcgtcctcg cggggcagat
     4441 tctgatggtg cagttcggcg ggaaggtctt cagaactgtg cccttaagcc ttgttgactg
     4501 gctgttgatc atcgtcggca cgtcgccggt gctgctcgtc ggcgagctgc ttcgcttcag
     4561 ggcgcggcgg cgggaaacgg cggcctgagt ttccccggcg ggggagaggg ttgcctgcac
     4621 gcggcggtcg ggagttctgc gggggaaccg cagggtttca cgcgacgaaa actcagcatc
     4681 gtgcgcacgc atgaacgaaa aaacagccgg agggcggcgg accgctctcc ggctgtactt
     4741 actgtgcggg ctggtctatg cttcttcgtt acgcgttgcc ctccgcctcg ttgctgtacg
     4801 taaagatggt gtcccacagc tcgtcgagct tgaccagcgt gtcgtcgatc gacgggacga
     4861 ggtaacggtc gccttccttc accagcaact tcttgtcctc cagcttgcga agcgcgtcgt
     4921 ccacctcaaa atccagcgtc acgccgaact tttctttcat ccactgctcg atgcggtcgt
     4981 ccagctcgtc ctgggagtac gcctttccct cggaatcctt ccctgtcagc aggaagtagt
     5041 aggcgagcag cgcttccttg cactcttcct cctccgcctc gtgaacgaga aagtcgaaga
     5101 ctccggcgtt gttgtccagg ttgtggaagt agagcgtctt catcagcttc gcctggtgct
     5161 tctccttcgt gagcttgtac ttcgtgaaga ccttgaaggc gatcgtcccg acgatggaga
     5221 aggccgcgag gatcgggaag agctgctcct tgaagtgcgc ccagtcgatg ttccgtccgg
     5281 cgacgatcgc ggcgatcagc g
//
LOCUS       DAOPKU010000167         5294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16863_length_5294_cov_4.701088, whole genome shotgun sequence.
ACCESSION   DAOPKU010000167 DAOPKU010000000
VERSION     DAOPKU010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16863_length_5294_cov_4.701088"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PK849_06495"
     CDS             <1..626
                     /locus_tag="PK849_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPE65813.1"
                     /translation="NIVSGSVPELRNNRLYNVTFLCRRDGTWDQQYKLHITPDEYRCW
                     GLQGGHDLKVFQTDVGKVGILICYDVEFPELARYLAIKGMDLLLIPFWTDTKNAYLRV
                     RRCAQARAIENECYVAIAGSVGNIPKVENMDIQYAQSAIFTPSDFAFPHDAVAAEATP
                     NTEMALIADLDVELLKELRLQGSVRNLHSRRTDLYWIDWLREGRNEE"
     gene            complement(742..2640)
                     /locus_tag="PK849_06500"
     CDS             complement(742..2640)
                     /locus_tag="PK849_06500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPE65814.1"
                     /translation="MKLFSSIALLALLTLLLPRPARADEPLIPMEDFFRNPEQSGFQL
                     SPNGEYISFMKPWERRMNIFVQKIGAEEAVRVTSATERDIPAYFWSGNDRIVYLQDRG
                     GDENYRLYAVSSDGTESRELTPFENTRVTIVDSLEEQDDFLLIGMNRRDQRLFDVYRL
                     NLATGDLTLLEENPGDIAGWMTDHDGKLRVAVRSDGVNTSLLYRDKEDEPFRNILTTD
                     FRESVSPLFFTFDNKKLYVASNLGRDKSAIFVFDPQTVEHEELLFEHPDVDVSSLFRS
                     RKRKVITGTGFTTDRFRYHFFDEQRAALQKELEERLPDDEVVVASMSRDETKVLVYAG
                     SDRTRGTYFFFDRETKDFRKLAELASWLPKERMAPMKHVSYTSRDGLTIHGYLTLPLG
                     KEPKNLPVVIHPHGGPWVRDGWGFDPQVQFLANRGLAVLQMNFRGSTGYGRAFWEAGF
                     KEWGRSMQDDVTDGVLWLINQGIADPKRVGIYGASYGGYAVLAGLAFTPDLYACGVDY
                     VGVSNIFTLLETIPPYWELGRQMLYEQIGDPEKDKELLKAASPVFHADRITAPLFVAQ
                     GANDPRVKKAESDQIVEALRSRGIEVEYMVKENEGHGFRNEENRFDFYRAMERFLATH
                     LGSLSEQH"
     gene            complement(2645..3058)
                     /locus_tag="PK849_06505"
     CDS             complement(2645..3058)
                     /locus_tag="PK849_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPE65815.1"
                     /translation="MKSHTEYLWFETKKRKELVHITPALQDILGRSGIGEGMMLVSAM
                     HITAGIIVNDNESGLHEDIMSWLERTAPEDPSYLHHRTGEDNGDAHLKRILIHHQAIL
                     PVTEGRLDLGPWERCFYAEFDGRRRKRVVVKVMGE"
     gene            3210..5270
                     /locus_tag="PK849_06510"
     CDS             3210..5270
                     /locus_tag="PK849_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65816.1"
                     /translation="MTEFSNDTLFDDGAAAMWRQDAGMLRAVLDVIPDIVSVQSPQLE
                     ILFYNKAGLDFLHASSEGTIGRNCYALLGRKEPCDDCLSLKAIRTGNAQEGEKYVPEL
                     DVWFEVRAIPVKDDAGSVRCVVEQLRDCTARKRNEERLARVLEELEAKNAELAETSAT
                     ARMLALRAEESSRAKSAFLANMSHEIRTPMNGILGMVELLLDTGLSMEQRDFGETISS
                     SADHLMDLLDDLLDFSKIEAERLTLESAPFNLQELVEDLTVQTAVKGREKNLDVYAVV
                     EPDVPAFFRGDASRLRQVLGNLLSNAVKFTDTGEISLAVSLAHDGILRTLLRFEVADT
                     GIGVPEEKKELLFKPFSQVDASITRRFGGTGLGLAISRGLVELMGGNIGMKDGQSGGA
                     LFWFEVPLDKIDHGDIEDLPVDPNLMNTPVLLVGGTPGWRRSVTTHLERWLCAVASAE
                     REGVEACLAGALDAERPFSVVLVDGSSVPEDNPFSPRALPERLRSVSSFYLLDYPSRR
                     WRRAALSDAGYADFVPKPVKRNALKNLLLSGTDSAGRIYSENTGAAPAGRKAKVLLVE
                     DSEVNRKVASVMLQRLGCSVSAAHDGFEALDFLRREAFDLVFMDVQMPVLDGIDAVKR
                     IRSGHGEAGRPDIPVIAMTARTLPGDREECFAAGMDDYISKPVKINVLEEMLEKWLPG
                     GEAQ"
BASE COUNT         1015 a         1581 c         1641 g         1057 t
ORIGIN      
        1 tcaacatcgt gtcggggagc gtgccggagc tgcggaacaa caggctctac aacgtcactt
       61 ttctttgccg ccgcgacggc acatgggacc agcagtacaa gctccacatc acacccgacg
      121 agtaccgctg ctgggggttg cagggggggc acgaccttaa ggtctttcag acggacgtcg
      181 gcaaggtcgg catcctcatc tgctacgatg tggagttccc ggagctggcg cgctacctcg
      241 ccatcaaggg aatggacctt ctcctcatcc ccttctggac ggacaccaag aacgcctacc
      301 tgcgcgtgcg ccgctgcgcc caggcccgcg ccatcgagaa cgagtgctac gtggccatcg
      361 caggcagcgt agggaacatt ccgaaggtgg agaacatgga cattcagtac gcgcagtccg
      421 ccatcttcac tccgtccgat ttcgcattcc ctcacgacgc cgtggcggcg gaggcgacgc
      481 cgaatactga aatggcgctg atcgccgacc tcgacgtcga actgctcaag gagctgcgtt
      541 tgcaggggag cgtgcggaat ctgcacagcc gccggacgga cctctactgg atcgactggc
      601 tgcgggaggg acggaacgag gagtagggga gggcggcagt gtccgaaaaa aatgagcgcc
      661 gctgcgcacg gttcggcgaa ccagcgggcg gcgctcttct tgtgtgctct catcgtcgcc
      721 tgaaagcgac ggggcggatg atcagtgttg ttcggagagg ctcccaaggt gcgttgcgag
      781 gaagcgctcc atagcgcggt aaaagtcgaa ccggttctcc tcgttgcgga agccgtgccc
      841 ctcgttctcc ttcaccatgt actcgacctc gatccccctg ctgcgcagcg cttcgacgat
      901 ctggtccgac tccgccttct tcaccctcgg gtcgttcgct ccctgggcga caaagagcgg
      961 cgccgtgatt ctgtccgcat ggaagacagg agaggccgct ttcagcagct ccttgtcttt
     1021 ttccgggtcg ccgatctgct cgtagagcat ctgccgccca agctcccagt agggggggat
     1081 ggtctccagc agcgtgaaga tgttcgaaac gccgacataa tcaaccccgc acgcgtacag
     1141 gtcgggggtg aaggcaagac ctgcgagcac ggcgtacccg ccgtaggaag cgccgtagat
     1201 gccgacccgc ttcggatccg cgatcccctg gttgatcagc cacagaacgc cgtcggtgac
     1261 gtcgtcctgc atcgacctgc cccactcttt gaaccccgct tcccagaacg cccttccgta
     1321 gcctgtggag ccgcggaagt tcatctgcag cactgcgagc cccctgttgg cgaggaactg
     1381 tacctgcggg tcgaaccccc atccgtcgcg tacccacggg cctccgtgcg ggtggatcac
     1441 cacggggagg ttcttcggct cctttccgag agggagcgtc aggtagccgt ggatcgtcag
     1501 cccgtccctc gatgtgtagg acacatgctt catcggcgcc atgcgctctt tcggcagcca
     1561 cgacgccagc tccgccagct tccggaagtc cttcgtctct ctgtcgaaaa agaagtaggt
     1621 cccgcgggtc cggtcgctcc ccgcgtacac cagcactttc gtctcgtccc ggctcatgga
     1681 ggcgacgacg acctcgtcgt caggaaggcg ctcttccagc tccttctgga gcgcggctcg
     1741 ctgctcgtcg aagaagtggt agcgaaagcg gtccgttgtg aagcccgttc cggtgatcac
     1801 ctttcgcttc cgggagcgga agagcgaact cacgtcgaca tccgggtgtt cgaagagcag
     1861 ctcctcgtgc tcgacagtct gcgggtcgaa gacgaagatc gccgacttgt cccgcccgag
     1921 gttcgacgcg acgtagagtt ttttgttgtc gaacgtgaag aagagcggcg acacgctttc
     1981 tcggaagtcc gtggtcagga tgttccggaa gggctcgtcc tccttgtcgc ggtagagaag
     2041 cgacgtgttc actccgtcgg agcgcaccgc gacccgcaac tttccatcgt ggtccgtcat
     2101 ccaccccgcg atatcccccg ggttctcctc cagcagcgtc agatctcccg tcgccaggtt
     2161 cagccgatag acgtcgaaga ggcgctggtc ccttcggttc atgccgatca ggagaaagtc
     2221 gtcctgctcc tcaaggctgt cgacgatggt cacgcgggta ttctcgaacg gcgtcagctc
     2281 gcgcgactcg gtcccgtcgg acgagacggc gtagagccgg tagttctcat cgccgcccct
     2341 gtcctgaagg tagacgatcc tgtcgttccc cgaccagaag taggccggga tatcccgctc
     2401 cgtcgcggag gttacgcgca cggcttcctc cgcgccgatt ttctggacga agatgttcat
     2461 ccgccgctcc cacggcttca tgaacgagat gtactccccg ttcggagaga gctggaagcc
     2521 gctctgctcc ggattacgga agaaatcctc catagggatg agaggctcgt ccgccctcgc
     2581 cgggcggggg agcagcagcg tcaagagcgc gagcagcgcg atggatgaaa agagtttcat
     2641 gatcttactc ccccatgacc ttgacgacca cgcgcttacg gcggcggccg tcgaactcgg
     2701 cgtagaaaca gcgctcccag ggcccgaggt cgagcctgcc ctccgttacc ggaagaatcg
     2761 cctgatggtg gatcaagatg cgcttcagat gggcgtcgcc gttgtcctcc cctgttctgt
     2821 gatgaagata ggacggatct tccggggctg tcctttccag ccagctcatg atatcctcgt
     2881 gaaggccgga ttcattgtcg tttacaataa taccggcggt gatgtgcatg gcggagacga
     2941 gcatcatccc ttcgccgata ccgctccgcc ccagaatgtc ctggagcgcc ggggtgatgt
     3001 gcacgagttc cttgcgtttc tttgtctcga accagaggta ttccgtatgg cttttcatac
     3061 tgatctctcc ttcgtgatgg tggctgtacg tggaatatgg tagcatactc ttttactgtc
     3121 gacccgtttt gtgctctatc ttgaaaagga agaaattcgc cgctctccga agggacggcc
     3181 tgagcggagg attgcgaggt gatgccgaaa tgaccgaatt tagcaatgat actcttttcg
     3241 acgacggcgc cgccgcaatg tggcgtcaag acgcgggcat gctgagggcc gtgctcgacg
     3301 tcatccccga tattgtttcc gtacagagtc cgcagcttga gattctcttt tacaacaagg
     3361 cggggctcga tttcctgcat gcgtcttccg aaggaacgat cggccggaac tgttatgcgc
     3421 tcctcgggag aaaagagccc tgcgacgatt gcctctcgct caaggccatc agaacgggaa
     3481 atgcccagga gggagagaaa tacgttccgg agcttgacgt gtggttcgag gtgcgcgcca
     3541 tcccggtgaa ggacgacgca ggtagtgtcc ggtgcgttgt ggagcagctg cgcgactgca
     3601 cggcaagaaa acggaacgag gagcgccttg cgcgcgttct cgaagagctt gaagcgaaga
     3661 acgcggaact tgccgagacg agcgcaacgg cccgtatgct tgcgctccgg gccgaggagt
     3721 ccagccgggc gaagagcgct tttctggcga acatgagtca cgagatacgc acgcccatga
     3781 acggaatcct cgggatggtc gaactcctgc tggatacggg gctctccatg gaacagcggg
     3841 atttcggaga gacgatcagc agcagcgccg atcatctgat ggatctgctc gacgacctgc
     3901 tggacttctc gaagatagaa gcggagcggt tgacgctgga aagcgccccg ttcaatcttc
     3961 aggaactcgt cgaggatctg acggtgcaga ctgcggtgaa aggacgggag aagaacctcg
     4021 atgtctacgc cgtcgtcgaa ccggacgttc ccgcattctt ccgcggggac gcctcgcgtc
     4081 ttcggcaggt gctcggcaac ctgctctcca acgccgtgaa attcaccgat accggagaaa
     4141 tctcgcttgc cgtctccctt gctcacgacg gtatcctcag gacgcttctt cgtttcgagg
     4201 ttgcggatac cggcatcggc gttccggagg agaagaagga gctcctgttc aaaccgtttt
     4261 cccaggttga cgcctccatc accagacgct tcggcggaac cgggctcgga cttgccattt
     4321 cgagggggct cgtcgaactc atgggaggaa acatcggtat gaaggacggg cagtccggcg
     4381 gcgctctttt ttggtttgaa gtccccctcg acaaaatcga ccacggcgac atcgaggatc
     4441 tccctgttga tccgaatctg atgaataccc ccgtcctgct tgtcggagga acgcccggat
     4501 ggaggcgctc cgtaacgacg cacctggagc gctggctctg cgccgttgcg tcggcggaac
     4561 gtgaaggggt cgaggcgtgt cttgccgggg ctctggacgc ggaacggcct ttttccgtcg
     4621 tgcttgtcga cgggagcagc gttccggaag acaacccctt ttcccctcgc gcgcttccgg
     4681 agaggctccg gagcgtttcg tcattctacc tgctcgacta cccttcaagg cgatggagga
     4741 gggcggcgct cagcgatgca gggtacgccg attttgtgcc caaacctgtc aagagaaacg
     4801 ccttgaagaa ccttctcctc tctggcacag acagcgccgg aaggatatac tccgagaata
     4861 cgggggctgc gccggccggc aggaaggcga aagtccttct cgtcgaggac agcgaagtca
     4921 accggaaggt ggcctccgtc atgctgcagc ggcttggatg ttcggtctcg gcggcgcatg
     4981 acggattcga agcgctggac ttcctgcgcc gcgaggcatt cgacctggtc ttcatggatg
     5041 tgcaaatgcc cgttctcgac gggatcgacg cagtgaagcg gattcggagc gggcacgggg
     5101 aagccggacg ccccgatatt ccggtgattg cgatgaccgc gcgcaccctg cccggcgaca
     5161 gagaagagtg ctttgcggcg gggatggacg actatatctc caagccggtg aagataaatg
     5221 tgttggaaga aatgctcgaa aagtggctgc ctggaggtga agcacagtga acagcggaga
     5281 acgacggatg cgta
//
LOCUS       DAOPKU010000168         5282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16912_length_5282_cov_4.554812, whole genome shotgun sequence.
ACCESSION   DAOPKU010000168 DAOPKU010000000
VERSION     DAOPKU010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16912_length_5282_cov_4.554812"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /gene="rimO"
                     /locus_tag="PK849_06515"
     CDS             complement(<1..1123)
                     /gene="rimO"
                     /locus_tag="PK849_06515"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPE65817.1"
                     /translation="MDSEKLLGVLQAFGVPVVHDIAEADIAIVNTCGFIQSAVEENVN
                     VILDLESMKEKGLLKKIGVVGCLVNRYGDELKKELPTVDLWAKAEEWGNVAAFFGGSM
                     TFEGKRGILQETRPWSRYLKVGEGCDTFCSYCTIPSIRGKARSIPLPELLQEAQSLVQ
                     NGAKELCLVGQDLTIYGRDLYGDDGLRTLLRALDAELPEEVWIRMLYLHPSRIDESFI
                     DMVAENPRILSYLDIPIQHIDDEILHRMNRPDKEGHIRRIFAYARSVDPLFALRTTII
                     TGFPGETERQFNKVLNFLEEAEIDRVGAFMYSPEEGTPAAQMEGQVDASVKNSRYDRL
                     MTLQAEISLERQRLFLGRTLSILVDEISQDDGFAWGRSYR"
     gene            complement(1165..2106)
                     /locus_tag="PK849_06520"
     CDS             complement(1165..2106)
                     /locus_tag="PK849_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HPE65818.1"
                     /translation="MPEERENMLQEAGREIQRKREQLGLSLEDVHEGTKIRINFLQGI
                     EQGDYSGFPGTVYVRGFIRTYLQFLGAEELWGDFLPMLSEGVEKKRPEEPVIGTCTPP
                     TRGFKPASKFWVFVLLLLIVLGSGWYVWYSWEQSGVSLFSLNNDRESVSEEERSENEG
                     NGRTSEQSLTLEPPAETSDPQSGEISGDSSMAIGAPDTHPTFVLPPTAAELVGVAETP
                     VATPTPVVAEKKEKELVITANADCWVRVRQGTKTVFERTMRSGDTADFKVTERLSVTF
                     GRGSAIKVTWNGKDLGAPGRGVERIFYDPDGTTGRFD"
     gene            complement(2099..2779)
                     /gene="rpe"
                     /locus_tag="PK849_06525"
     CDS             complement(2099..2779)
                     /gene="rpe"
                     /locus_tag="PK849_06525"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPE65819.1"
                     /translation="MNRNLFFAPSLLAADILNMERSISSLEGEHDWLHVDVMDGCFVP
                     NITFGPGFVSALRKRFPQETLDVHLMIEHPELLLDDFLAAGADYLTIHAEADHHIHRT
                     LSRIRERGGKAGISINPGTSEEILRPLLPFVDLVLVMSVNPGFGGQSFIGSVMEKVTM
                     LSRWRTAGHYSYLIEMDGGIGRQNAAMVALGGTDVLVMGSAVFGTPEPARTLREIRTL
                     VKEATDRA"
     gene            complement(2776..3948)
                     /locus_tag="PK849_06530"
     CDS             complement(2776..3948)
                     /locus_tag="PK849_06530"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPE65820.1"
                     /translation="MIGKILRWAIVLALLVILISGSVAFYTVFFGGKDLVIPPLREMS
                     VLDAVESAERMGLEVRIEQIDSSVPVGTVLAQWPDPGTKVRKDKTVILKVSKGGNRQA
                     ITDLRGLEQGQALRMIEEQGFSAGEILRVHDGNRPAGTVIAQNPASPAMIEGGRKIEL
                     LISMGPLPKDGRIPVPDLAQRDEKTARELLLQSGLRVGGVEYVNTQHTPDGMVMATRP
                     KTGAMIRQGDAVVIQVATSRRKEPELVPETRAATPTPPPQGEVVVEQPQTPVRQIGPA
                     GPVQTLTPEQQELAGAIGLPVDRPPAAATPTPSTPPAVRPGPASTGTARIRYQVPPLT
                     KPLSLKIEMVDATGTKALHGRDVKGGEFISLDAPFVTEAVVTVYLGGEFVWQERYK"
     gene            complement(3945..>5282)
                     /locus_tag="PK849_06535"
     CDS             complement(3945..>5282)
                     /locus_tag="PK849_06535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HPE65821.1"
                     /translation="LALRGIEAALHVWSEVREGKFASEVIRRLADSMTPGDLMLASTL
                     VYTALRRQFLWKEIYAHFLKASPAGVSRLTDDALCIGTAGLLELRKFAPRVLVNALVQ
                     YMKDKGDERGARLVNAVLRRVSEEGRKELARLESSGNSKEQALVAGIPPWVASYWRNT
                     IGDEAKKLLKMARIRPYSSFRILPEDKMDAILEQAKNSGICCWPSPLVPYSIRLSSTI
                     YPGNFPGYSEGWSTPQSESSMLVGEVMKKVYKGGAILEMCSGRGVKTGHIARLFPEAH
                     IECVELSQGRVGAAVREMKRLNIRRLPVFTAADALHAEPASSPAFISLDAPCSGSGTW
                     NRHPEGKWRLSAEKLESLALLQRNLLKRAVSLLAPGGIVVYSTCSLLKTENENVVAGI
                     LSEDASLVELSFPFSAKYFRRGRPWGVYIWPELPWLDGFYMAVIMKRSGGKDC"
BASE COUNT         1306 a         1474 c         1206 g         1296 t
ORIGIN      
        1 ctctgtagga acgcccccat gcaaatccgt cgtcctggct gatttcatcg accagaattg
       61 aaagcgtacg tcctaaaaaa agtcgttgtc tctccaggga tatctcagcc tgtagcgtca
      121 ttaaacggtc gtagcggctg ttttttaccg acgcatcgac ctgtccctcc atttgagccg
      181 ccggcgttcc ctcttcggga gaatacataa atgcaccaac gcgatctatt tcggcctctt
      241 caagaaaatt gagcactttg ttgaactgtc gctcggtttc ccccgggaaa ccggtaataa
      301 tcgttgtacg aagcgcaaag agcggatcga cgctcctcgc gtaagcaaat atacgacgaa
      361 tgtgtccctc tttgtccgga cggttcattc tgtgcaaaat ctcatcatca atgtgttgta
      421 tcggtatgtc cagataggac aaaattctcg gattctcggc aaccatatca atgaatgatt
      481 cgtctattct tgaaggatga agatacagca tgcgaatcca aacttcttcc ggaagctcag
      541 cgtcaagagc tcgcaaaagc gtacgaagtc catcatctcc gtaaagatct cttccgtaaa
      601 ttgtaagatc ctgtcctaca agacatagtt ccttcgctcc gttctgaaca aggctttgcg
      661 cttcttggag aagttcagga agaggaatgc tgcgagcctt tcctctaatc gaagggatag
      721 tgcagtaaga acaaaaagtg tcgcaccctt cacccacttt tagatagcgt gaccaagggc
      781 gcgtttcttg tagaataccc cttttcccct cgaaggtcat acttcctccg aaaaaggcgg
      841 caacgttccc ccactcttca gcttttgccc acaaatcaac cgttggaagc tcttttttca
      901 actcgtcgcc atatctgttg acaaggcaac cgactacgcc gattttttta agaagccctt
      961 tttctttcat tgactcaaga tcgaggataa cattaacatt ctcctcgacg gcagactgaa
     1021 taaagccgca tgtgttcaca atggcgatat cggcctcggc aatatcgtgc acaacaggca
     1081 ctccgaaagc ttgaagaact ccgaggagct tttcgctgtc cacggagttc tttgcgcatc
     1141 ctaaactgat tagataaact ttcactaatc gaaccttccc gttgttccgt ccggatcata
     1201 aaatattctt tcgactcctc gtccgggagc ccctaaatcc ttgccgttcc atgtcacttt
     1261 aatggcgctt ccccgtccaa aagtaacact cagtctttca gttaccttaa aatcggcagt
     1321 atctccggat cgcatcgtac gctcaaacac cgttttggtt ccttgacgaa cacgtaccca
     1381 gcaatcggcg ttggccgtga taaccagctc tttctccttc ttttctgcaa ctacaggcgt
     1441 cggcgtagca acaggtgttt ccgcaacgcc tacaagctcc gcagctgtgg gagggagaac
     1501 aaaagtcgga tgcgtatcag gagccccgat tgccatagat gaatctccac tgatttcacc
     1561 tgattgagga tcagacgttt cagcaggggg ttcaagggtt aggctctgtt ctgaagttcg
     1621 tccgttccct tcgttttcag atcgttcttc ttcggacaca gattcccgat cattgttcag
     1681 tgaaaagagg gaaacgccgc tctgttccca cgaataccaa acataccatc ccgaccccaa
     1741 aacgatcagc aaaagaagaa cgaaaaccca aaatttagaa gcaggtttaa accccctcgt
     1801 cggaggggta catgttccaa taacaggctc ttccggtcgc tttttttcga ctccctctga
     1861 aagcatcgga agaaaatcgc cccagagttc ttccgcgcca aggaactgca gatatgtccg
     1921 gataaatcct cgaacgtaca ctgttccagg aaaacccgag tagtctcctt gttcgattcc
     1981 ctgtaaaaag ttaattcgta tttttgttcc ctcgtggacg tcctcaagac taagtcccaa
     2041 ctgttctctc ttgcgttgaa tttctcttcc agcctcttgg agcatgtttt ctctttcttc
     2101 aggcacgatc tgttgcctcc ttaacaagtg ttcgtatttc gcgcagggtt cgggcaggtt
     2161 caggggtacc aaagaccgcg cttcccatta caagaacgtc ggtccctccc agagccacca
     2221 tagctgcatt ctgcctcccg ataccaccgt ccatttcaat gagataggaa tagtggcctg
     2281 cggttctcca tctggagagc atggtcacct tttccatgac ggatccgata aatgattgtc
     2341 ccccaaaacc aggattaaca gacatgacaa gaacaagatc aacaaatgga agcaacggac
     2401 ggagaatctc ctccgaagtt cccgggttga ttgaaatacc ggccttgccc cccctttccc
     2461 gaatccgcga cagggtcctg tgaatatgat gatccgcttc tgcatgaatg gtcagataat
     2521 cggcgcctgc tgccaaaaaa tcatcaagaa gcaattcagg gtgctcgatc atgagatgga
     2581 cgtcgagagt ctcctggggg aatctcttcc gcagcgcaga aacaaatccc gggccaaagg
     2641 ttatattcgg tacaaaacat ccgtccataa catccacgtg gagccaatca tgttcgcctt
     2701 ccaacgaaga gatagaacgc tccatattga ggatatccgc agcgaggaga gaaggtgcaa
     2761 agaaaaggtt cctgttcatt tatatcgctc ctgccagaca aattcgccgc caagatacac
     2821 agtaacaact gcttctgtta caaagggagc gtcgagagaa atgaattctc cgccttttac
     2881 atcgcgcccg tgcagcgctt tcgtccccgt tgcatcgacc atctctatct taagagagag
     2941 cggcttcgtc aatggaggca cctgatatcg tattctcgcc gttcctgtcg aagcgggtcc
     3001 tggacgaaca gcgggagggg tagaaggcgt cggtgtcgct gctgccggcg gtctgtctac
     3061 aggcaaacct atggctccgg caagttcctg ctgctccggg gtcaatgttt gtacaggccc
     3121 ggccggtcca atttggcgaa cgggcgtctg cggttgttct acaacgactt caccctgcgg
     3181 gggcggagtc ggcgtggccg ctcgagtttc cggaaccagt tccggttcct tccgcctgct
     3241 tgttgccacc tgaataacaa ctgcgtctcc ctggcgtatc attgccccag ttttcggcct
     3301 ggtcgccatg accataccat ccggggtgtg ctgcgtattc acatattcaa caccgcccac
     3361 gcgaagaccg ctttgcagca aaagttctct tgcagttttc tcatcgcgtt gtgctaaatc
     3421 cggaacggga atacgtccgt ctttgggcag aggccccatg ctgataagga gttcaatttt
     3481 tcttcctcct tcaatcatag caggagaagc gggattctga gcgattacag tgcctgcagg
     3541 acgatttccg tcgtggacac ggagaatctc acctgcgctg aacccctgct cttcaatcat
     3601 ccgcaacgct tgcccctgtt ctagtcctcg caaatcagtg attgcttgcc tgtttccgcc
     3661 cttactgacc ttcagtataa ccgttttatc ttttcgcact tttgtaccgg gatccggcca
     3721 ctgggcaaga accgtaccga cgggaacaga tgaatcaatc tgttcaattc tgacttccaa
     3781 gcccatgcgc tcagcgcttt ctaccgcatc aagcactgac atctcccgca gcggcgggat
     3841 caccaggtct ttccctccaa aaaaaacagt gtaaaaggcg acgcttccgg agataagaat
     3901 gaccaacaac gccagtacta tggcccagcg taatattttt cctatcaaca gtctttccct
     3961 cccgatctct tcatgatcac cgccatgtaa aaaccatcaa gccaaggcag ttcaggccag
     4021 atgtagactc cccagggacg tcctctacga aagtatttcg cggaaaaagg aaaagaaagt
     4081 tcgacaagag aagcgtcctc cgaaagaatt ccggcgacta cattttcatt ttctgtcttc
     4141 aaaagactac atgtagagta gaccacaatc cctcccggcg caagaagaga aactgctctt
     4201 tttaaaaggt ttcgctgaag cagcgctaaa gactccagtt tttctgcgga aaggcgccac
     4261 ttgccctccg gatgccggtt ccatgtgccg cttcctgaac agggagcatc aagtgaaatg
     4321 aaagcgggag aactcgcagg ttctgcatgc agggcgtccg ccgcagtaaa aacaggaaga
     4381 cgtctgatat tgagacgctt catctcccgg accgcggctc cgacacgtcc ttgagacagt
     4441 tccacgcact caatgtgagc ttcgggaaaa agtcgcgcga tgtgtccggt tttcacgccg
     4501 cgtccagagc acatctccaa aatagctccg cccttgtata cttttttcat cacctcgccg
     4561 acgagcatcg aggactccga ctgaggcgta gaccatccct cggaataccc ggggaagttg
     4621 cccgggtata tggtggagct gaggcgaatt gaatacggga caagagggga aggccaacag
     4681 caaatgccgc tgtttttagc ttgttccagg atagcgtcca ttttgtcctc cggaagaatg
     4741 cgaaacgaag aatatggacg aatacgcgcc attttcagga gtttttttgc ctcatcgccg
     4801 atagtgttcc tccaatacga agcaacccac ggggggatac ctgccacaag cgcctgttcc
     4861 ttgctgttgc cggaagactc gagccgcgca agttcttttc tcccttcttc agacaccctt
     4921 cgaagaacgg cgttcaccag ccgagcgcct ctttcgtccc ctttatcttt catatactgc
     4981 acaagagcat ttaccaaaac acgcggagca aacttcctca gctcaagcaa acctgcggtc
     5041 ccaatgcaaa gagcatcgtc tgtaagacga gaaacgccgg cgggagacgc cttcaaaaaa
     5101 tgcgcgtaga tctctttcca gagaaactgt ctcctgaggg ctgtatacac aagggtcgac
     5161 gcaagcataa gatctccagg cgtcattgaa tctgccaggc gtctgataac ctcggaagcg
     5221 aatttgcctt cccgcacttc actccagaca tgaagagcag cctcaatccc tctcaaggcg
     5281 ag
//
LOCUS       DAOPKU010000169         5267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_16986_length_5267_cov_4.761320, whole genome shotgun sequence.
ACCESSION   DAOPKU010000169 DAOPKU010000000
VERSION     DAOPKU010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16986_length_5267_cov_4.761320"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..417)
                     /locus_tag="PK849_06540"
     CDS             complement(106..417)
                     /locus_tag="PK849_06540"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HPE65822.1"
                     /translation="MLSNEEEWKALWSRINGNIQPLPPLPEVNFSTHAVAAVFQGRKN
                     SGGYSIGVEAVIEMPDRVTVTVRECEPGPSSIVTSALSSPWEAVAFPLPEKPVLFTSI
                     N"
     gene            complement(691..2181)
                     /locus_tag="PK849_06545"
     CDS             complement(691..2181)
                     /locus_tag="PK849_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPE65823.1"
                     /translation="MSIRLEEVIGKRLAGDQSQRVIWWKGTWWDAEKVEQKTEECASI
                     LREAGFQPGSRLAAFLPNSPLVLLLSMAAWRLGGTFAPLNPRGGVEWILRILDHLDPF
                     AVVVGEEMTPLEEAIRGAGIPVVAAPSDGVPPAFTGKRDRIGQDPDIAVIFATSGTTG
                     APKAVPLTHENLYDNTKGVHETIEGFDKGRVLMNVLPNFHSFGYTVCSVLPLLWGLPE
                     ALLPGFMPLKNLFEGLRDSGTGILIAVPTMLPFILGAASKGEKLPSELRYILTGGGKL
                     DPLLEKRFRDQLGVITYEGYGLTECSPVVSCNPSDALRKTGTVGPALPSYTVEVRGED
                     GRPVPPGQDGVLWLKGPSVAGGYFRDPELSAERFHDGWIDTGDMVRVDEDGYISILDR
                     VSDMIIVGGFNVYPQEVETVIKEIPGVKEAVVVGVSNPVSGEVPMAFVIKEETADLES
                     NTIISYCKEKMAHFKVPRKVKFVTELPLSAVGKVLKRKLREQMVRE"
     gene            complement(2251..3480)
                     /locus_tag="PK849_06550"
     CDS             complement(2251..3480)
                     /locus_tag="PK849_06550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPE65824.1"
                     /translation="MNAHASPVLLPGRGSERRNEIRRWCFLVLSVLLLFLSCAGRAVA
                     AAGLLPAEHAEESGSPEKISRFSFLAVGDALIHKGVYEAARKGKKQFDFRPMFRLVKE
                     RVQAHDVAFYNQETILGGVDLRLSTYPCFNSPQEVGDGMIDAGFNLVSLANNHTLDRG
                     EKAILASHEYWAGQDGVLTAGSYASLEDRETPRLIEVNGITCAFLAYTTLTNGLKAPK
                     DKEFYVAIYDKDSVEADVKRAREAKADVVIVSMHWGSEYVFTPGAQQKEIAVHLASLG
                     VNLVVGHHPHVVQPVQMVKDTLVIYSLGNFISSQKELFKLIGLMVSLDVVKKERGKEK
                     TISFENVTGTLLYNPRRSPLGRYSVVPFDLMKPDILKDFDKVHKKYAAYVKLDETLSI
                     RPPAKMPPKPPAKRRKK"
     gene            3696..4847
                     /locus_tag="PK849_06555"
     CDS             3696..4847
                     /locus_tag="PK849_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE65825.1"
                     /translation="MKKAPWILAVLLVATLVTGSCAEEVIKVGYLAGITGDFAAYGQP
                     EVNAVKLAADEINAKGGVLGKKIELVIYDYKSRPEDAVNAARRLIDHDKVVAILGSAA
                     SGANIATAPLVTRAGIPQIGTLSTNPLVTVDESGNVRPYMFRICFIDPYQGRLIGYFA
                     AKELGKKKAAILYDVGSDYSHGLREHFISSYEEFGGTVVAEHAFRGGQDVDFRAQLTQ
                     IKQTDAEILVLPNIGKDLALPIKQARELGMEDIIFIGGDGYGEFMVEIAGEALEGSYW
                     MYHLARQDPAMKPFFDAYKEKYNDECQEFAIAVLAYDSLYWLVDAIERAGSADSKAIR
                     DALEATKDLQLHHATLTMDPKTHNPVDKDAVVLTVQGGKSIFYKKIKPE"
     gene            complement(4957..>5267)
                     /locus_tag="PK849_06560"
     CDS             complement(4957..>5267)
                     /locus_tag="PK849_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE65826.1"
                     /translation="FAQNGITVESATSANPLYMPPESRLIKALQKAYTEVTGEEAELL
                     AIGGGTYAKTMPNVVAFGPVFPGQTYKIHEEDECWSVEDIMKNAHIMAKVLVELAERK
                     "
BASE COUNT         1128 a         1572 c         1481 g         1086 t
ORIGIN      
        1 aagaaggtag ctgatacgcg cgtcccgttt tttacggaag agggcgtccc gaaagactgc
       61 gggacgccct cttcttgctg gctctctttg aagtaggtcc ggatatcagt tgatcgaggt
      121 aaaaagaacg ggcttctccg gcaaagggaa ggcaaccgcc tcccatgggc tcgaaagagc
      181 gctcgtcacg atgctcgacg gaccgggttc acactcccgt accgttacgg ttacgcgatc
      241 cggcatttcg ataaccgctt ctacgccgat agagtaccct ccgctgtttt tcctgccttg
      301 gaaaaccgcg gccaccgcat gtgtggaaaa attcacctcg gggagaggcg gcaacggctg
      361 tatgttgccg ttgattctac tccacaacgc cttccactct tcctcgttcg aaagcacaag
      421 aaatgaacgt tcttttactc ccccgtaact tcctccggcg atcttgctga aagagagaac
      481 tgcgccatcg tcgtctcctc aggctacgca tgcaaaaaca gtatctacag aagagagaga
      541 cagaaacaaa agagcagcgc acgcaacacc aaacaccatc ttcatccgtt tcacatgcta
      601 cccctccagc tcaaacaagt ctgttgtgaa agaataacag accagagcgt ttccctgatg
      661 ggtaaaagag cgtattcgtc atccggcgct ctattcgcgt accatctgct cccggagctt
      721 cctcttgagc accttgccga ctgcggacag cgggagctcc gtcacgaact tcaccttgcg
      781 gggaaccttg aaatgcgcca tcttctcttt gcagtaggag atgatcgtat tcgactcaag
      841 atcggcggtt tcttctttta taacgaaggc catcggcact tcgccgctga cgggattcga
      901 tacgcccacc acgacggcct ctttcacgcc ggggatttct ttgatgaccg tctccacctc
      961 ctgcgggtag acgttgaagc cgccgacgat aatcatgtcg gagaccctgt cgaggatgga
     1021 gatgtacccg tcctcgtcca ccctgaccat gtcgccggtg tcgatccacc cgtcgtggaa
     1081 gcgctcggcg ctgagttcag gatcgcggaa atagcccccg gccaccgacg gtcctttaag
     1141 ccagagcacg ccgtcctgcc cgggagggac aggacggccg tcctcccctc gaacttccac
     1201 agtatagctc ggcagcgcgg ggccgaccgt tcccgtcttc cggagcgcgt cgctcggatt
     1261 gcacgagacc acgggggagc attcagtaag cccgtaccct tcataggtga tgacgccgag
     1321 ctggtcgcgg aagcgttttt cgagcagcgg atcgagcttt ccgccgccgg tgaggatgta
     1381 ccgcagctcg gacgggagct tctccccctt tgaggcggcg cccaggatga acggaagcat
     1441 cgtgggaacg gcgatcagga ttccggtccc ggagtcgcgc agtccttcga agaggttttt
     1501 caacggcatg aagccgggga gcagggcttc cggaagtccc cagagcagcg ggagcacgct
     1561 gcacaccgtg tagccgaagg agtgaaagtt cgggaggacg ttcatcagga cgcgtccctt
     1621 gtcaaaaccc tcaatggttt cgtggacgcc cttcgtgttg tcgtacagat tttcatgggt
     1681 gagcggcacc gctttcggcg cgccggtcgt ccccgaggta gcaaaaatca ccgcaatatc
     1741 gggatcttgg ccgattctgt cgcgtttgcc ggtaaacgca ggaggaacgc cgtccgaggg
     1801 agccgcgacg acgggtatcc ctgcgccccg gatggcttct tcaagaggcg tcatttcctc
     1861 tccgacgacg accgcgaacg gatccaggtg atcgagaata cgaagaatcc actcgacgcc
     1921 gccgcgcgga ttcaggggcg caaaagtccc tccaagccgc caggcagcca tggagagaag
     1981 gaggaccagc gggctgttgg gaagaaacgc cgcaagccgg cttcccggtt ggaaccctgc
     2041 ttcacggagt atggacgcgc actcctcggt cttttgctca accttctccg catcccacca
     2101 tgttcccttc caccagatca cccgctgcga ctgatccccg gcaagacgtt tcccgattac
     2161 ttcttcaagt cgaatggaca cacaaaaccc tccctgtctc ggcgaaggcg cactctgtcg
     2221 agggctccct tcagaataaa cagacgcata tcactttttt ctccgtttcg ccggaggctt
     2281 cggcggcatt tttgcaggag gacggatcga aagcgtctcg tccagcttca cgtatgccgc
     2341 gtacttttta tgaaccttgt cgaaatcttt caggatatcg ggtttcatca ggtcgaacgg
     2401 cactacggag taccggccga gcggcgaacg cctcgggttg taaagcagcg tccccgtcac
     2461 gttctcgaag gagatggtct tctctttccc ccgctctttc ttcaccacgt cgagcgacac
     2521 catcagcccg atcagtttga agagctcttt ctgggaggag atgaaattcc ccagcgagta
     2581 gatcacaagg gtatccttca ccatctggac aggctgcacc acatgagggt gatgcccgac
     2641 gacgagattc actccgagcg aggccagatg cacggcgatc tccttctgct gcgctccggg
     2701 cgtaaagacg tattcgcttc cccagtgcat ggagacgatg acgacatccg ctttcgcctc
     2761 cctggcccgc ttcacgtccg cctcaactga atccttgtcg tagatggcta cgtagaactc
     2821 tttatccttg ggcgccttaa gaccgttcgt gagcgtggtg tacgcgagga aggcgcaggt
     2881 gatgccgttt acctcgatga gcctcggcgt ttcccggtcc tccagcgacg catagcttcc
     2941 ggccgtcagg actccgtcct gccccgccca gtattcgtgc gaggcgagga tcgccttctc
     3001 cccccggtca agcgtgtggt tattcgccag cgagacgagg ttgaagccgg cgtcgatcat
     3061 accgtcgccc acttcctgcg gagagttgaa acacgggtac gtggacaggc gcaagtcaac
     3121 gccgccgagg atagtctcct ggttgtagaa ggcaacgtcg tgcgcctgaa cccgttcttt
     3181 tacaagccgg aacatcgggc ggaaatcgaa ctgttttttc ccctttcgcg ccgcttcgta
     3241 cacgccttta tgaatcaacg cgtcgccgac ggcgaggaag gaaaagcgtg agattttctc
     3301 agggctcccg ctctcctccg cgtgctctgc aggaagaaga ccggcggcgg caaccgcccg
     3361 tcccgcgcac gaaagaaaga gaaggagaac tgagagaacg agaaaacacc atcgccggat
     3421 ttcatttcgg cgttcgctcc ctctccccgg caacaggacg ggggaagcgt gtgcattcat
     3481 caaaggacca cctccggcaa aagctctatg tctattatac cctcatgaac ggagaaatag
     3541 gcttctcttc cgttgcgaat ccaacttatc gaacttctcc attgacaaag agagtctcag
     3601 agattagcat atcgaatatc ggatactata tcggatcggt cgtgcggagc tcttcttttc
     3661 gcataaccgc aataattccc ggaggtgagt gcttcatgaa aaaggcgcca tggattctcg
     3721 cggtactttt agtagcaacc ctcgtaacag gttcgtgtgc tgaagaagtt atcaaggtag
     3781 ggtatcttgc aggaatcaca ggagactttg cagcgtacgg tcagccggaa gtgaacgcgg
     3841 tcaagctcgc ggcggacgag atcaacgcaa agggcggcgt tcttggcaag aagatcgaac
     3901 tggtcatcta cgactacaaa tcccgccccg aagatgcggt caacgccgcc agacgcctga
     3961 tcgaccacga caaggttgtg gcgatcctcg gctccgcggc cagcggcgcc aacatcgcga
     4021 ccgcgccgct cgtcacgcgc gccggaattc cgcagatcgg cacgctctcc actaacccgc
     4081 ttgtcaccgt tgacgagagc ggaaatgtcc gtccgtacat gttccgtatc tgcttcatcg
     4141 atccctacca ggggcgtctg atcggatact ttgcagccaa ggagctcggc aagaaaaagg
     4201 ccgcgatcct ctacgacgtt ggaagcgact actcccacgg cctgcgcgag cacttcatca
     4261 gcagctacga agagttcggc ggcacggttg tcgcggagca cgcgttccgc ggcggccagg
     4321 acgtcgactt ccgggcccag ttgacgcaga tcaagcagac ggacgccgaa atactggttc
     4381 ttccgaacat cgggaaggac ctggcgcttc cgatcaagca ggcgagggag cttggaatgg
     4441 aagacatcat cttcatcggc ggcgacggtt acggcgagtt catggtcgag atcgcggggg
     4501 aggctcttga gggttcgtac tggatgtacc atctcgcccg tcaggacccg gcaatgaagc
     4561 ccttcttcga cgcttataaa gagaagtaca acgacgagtg ccaggagttc gccatcgccg
     4621 tgctcgccta cgacagcctc tattggctcg tcgacgcgat cgagcgcgcc ggaagcgctg
     4681 attcaaaggc catccgcgac gcccttgaag caacgaagga tctgcagctt caccatgcga
     4741 cgttgacgat ggacccgaag acccacaacc ccgtggacaa ggacgccgtc gtgctcaccg
     4801 tccagggcgg aaagagcata ttctacaaaa agatcaagcc ggaataacat cggattcggc
     4861 agcagtgcat gaacaaggag aggggagagc ccgggaatac cggctctccc ctctttgtac
     4921 gcgtgctgtg tactacggcc cccctgcgag cgcaggttac ttcctctcgg cgagttcgac
     4981 gagaactttc gccatgatat gagcgttctt catgatatcc tccaccgacc agcactcgtc
     5041 ttcctcgtga attttgtagg tctgtccggg gaagacgggg ccgaaggcca cgacgttcgg
     5101 catggttttg gcgtaggtgc cgccgccgat ggccagcagc tcggcctctt cgccggtcac
     5161 ctcggtgtag gctttctgca gcgccttgat gaggcgggac tcgggaggca tatagagagg
     5221 attggccgag gttgcgcttt cgacagtgat accgttctgc gcaaatg
//
LOCUS       DAOPKU010000170         5189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_17346_length_5189_cov_4.705882, whole genome shotgun sequence.
ACCESSION   DAOPKU010000170 DAOPKU010000000
VERSION     DAOPKU010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17346_length_5189_cov_4.705882"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="PK849_06565"
     CDS             complement(<1..1298)
                     /locus_tag="PK849_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE65827.1"
                     /translation="MDWESWLRGEEFPPLKQEDPNKNSGASLAELLGLPVVLPEETAE
                     NRSPAASAGEPLQVSQTGGSVDVEVSDADGTMSSLPQATEIQGESSGQQDVMFPVVSE
                     VVEKTETRRYVPASVSEGEPQETKVSEATEEGSSSDTENTDQTALPETERHDDAFVSS
                     GRTEEPLAPSIEPDETGVSVSVQEESGESRSAVSTFTIPAHPLYPLIEEDSAPEKTIF
                     VETNRTHAQPQREKNTKNRVLPICLLAAALVMGGLFFASGESFEKILQQGDQAYAQKE
                     YDRALLLYEKAAEKNPLRAEPLFGKARTLEELDRKGEAVHAWNSCLKIAPEDAEVYRR
                     LGDLLFELGSLDNAIRNYQESVRFEPEDSETHFRLGSLLESREDFSQALESYRKALEV
                     DATRDDFAEAVERTEKKVLIADEERRQRTALSEEQIRLGAA"
     gene            complement(1462..1548)
                     /locus_tag="PK849_06570"
     tRNA            complement(1462..1548)
                     /locus_tag="PK849_06570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1512..1514),aa:Leu,seq:aag)
     gene            complement(1600..1947)
                     /gene="rplS"
                     /locus_tag="PK849_06575"
     CDS             complement(1600..1947)
                     /gene="rplS"
                     /locus_tag="PK849_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPE65828.1"
                     /translation="MNVLNLVEKKFLKSDVPEFRSGDTVRVHVKVKEGSRERIQVFEG
                     VVIARKHGGLSETFTVRKISSGVGVERIFPLHCPSISQIEVKRLGKVRRAKLYYLRDR
                     SGKSARIKERREF"
     gene            complement(2103..3365)
                     /gene="trmD"
                     /locus_tag="PK849_06580"
     CDS             complement(2103..3365)
                     /gene="trmD"
                     /locus_tag="PK849_06580"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPE65829.1"
                     /translation="MHVTIVTAFPEFFEKFLSTSMIGRGIKNGLLHVDVVDLRNFGLG
                     NYRQIDDYAFGGEGGMVLLPEVLERALAAIRESSGTKGYVVYPSPQGDLFSQEIVETL
                     AAQEHIILFCGHYEGLDERFVSRCVDREVSMGDFVLTGGEIPAMAIIDAMARLVPGVV
                     GKETAVVEDSFFRGMLDHPHCTRPAEWNGECVPEELLSGDAAAIENWRRNAAVERTLA
                     RRPDLISRANIRPYLPKGIYVELVHFPVLDRRGEKSTAAVTGLDLSDIARSCKTYGID
                     RFFVTTPLASQRDLVKTMTRHWTEGGGANVNPERAEAFRLLKLFPSVAKSVEWILKRE
                     KEIPLIVGTTARKHPGAFHWIEAKRRMLREKRPILLLFGTGSGLHEEVLAECSMVLSP
                     LSGGGGEYNHLSVRTAAGAVLDRFFGWR"
     gene            complement(3356..3886)
                     /gene="rimM"
                     /locus_tag="PK849_06585"
     CDS             complement(3356..3886)
                     /gene="rimM"
                     /locus_tag="PK849_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPE65830.1"
                     /translation="MNRVRIGRIVGTHGLRGALKIVPLTDYPERFHSMEKMSVYSAEG
                     QIITTLTLLSSRYSDHKKLLVIQTEELRRIEDAEPLSGAFIEILPEERYPLGEGEYWI
                     EDLKGLSAVRHEDGASLGKLVDVATAGENDIYVIRDDSGNDHYIPAVKQFVAEVNLEK
                     KEIRILLIEGLWESCM"
     gene            complement(3879..4127)
                     /locus_tag="PK849_06590"
     CDS             complement(3879..4127)
                     /locus_tag="PK849_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPE65831.1"
                     /translation="MPDYRELVEFIVRRLVTQPDEVQISEQTDERGVTAITIRVAPED
                     VGRVIGKRGATINAIRLVAKASAVKVNDRVDVDIVEDE"
     gene            complement(4130..4405)
                     /gene="rpsP"
                     /locus_tag="PK849_06595"
     CDS             complement(4130..4405)
                     /gene="rpsP"
                     /locus_tag="PK849_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPE65832.1"
                     /translation="MAVRIRLARHGRKKAPFYRLVVADSRSPRDGRFIELLGTYDPMK
                     DPSSINVDEERAMHWLKVGATASDTARSILRKSGVWEKFEAGKKQAE"
     gene            complement(4485..>5189)
                     /locus_tag="PK849_06600"
     CDS             complement(4485..>5189)
                     /locus_tag="PK849_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPE65833.1"
                     /translation="RLVVDAMTGQEAVKVASSFHEKLQLTGVVLSKLDGDARGGAALA
                     IRKVTGVPVKLAGVGEATDALEVFDAKRMAQRILGMGDVIGLAEKIQEMADSGGAERM
                     AETLKKKFTLEELLAQFEQLEKMGPLNKVLEMIPGFSKMKGVSQDEVDSGRLKKSKAI
                     IQSMTRAERRDPSIIKGSRRRRIAMGSGTSVQMVNQLLAQYEQMRKLWKQFGKQGKGF
                     KLPKGLLGGGGGFRFK"
BASE COUNT         1180 a         1537 c         1210 g         1262 t
ORIGIN      
        1 gcggctccaa gtcgaatttg ctcttccgac agagcggtcc gctgacgacg ctcctcatcc
       61 gcaatgagaa cctttttttc ggtcctttcg acagcctcag caaagtcatc tctagtagca
      121 tctacttcca aagcctttcg atacgattcg agggcttgag aaaaatcctc tcttgattcc
      181 aaaagggaac cgagccgaaa atgagtttcc gaatcctccg gttcaaatct gacgctctct
      241 tgatagttcc taatcgcgtt gtccaaggaa ccaagttcga aaagcaaatc gcccagacgc
      301 ctatatacct ccgcatcctc gggagcgatc ttcagacagc tgttccatgc gtgaacggct
      361 tctccctttc tatccagctc ttcaagagtt cgcgctttgc caaagagggg ttccgcacga
      421 agagggtttt tctccgccgc tttttcgtaa agaagcaacg ccctgtcgta ctctttttgc
      481 gcgtaagctt ggtcgccttg ctgcaatatc ttttcaaacg attctccgga agcaaaaaaa
      541 agtccgccca ttacaagcgc tgccgccagc aaacaaatcg gcaacactcg gttttttgtg
      601 tttttctcac gctgcggctg ggcgtgagtc cggttcgttt cgacaaaaat cgttttttca
      661 ggggcagaat cctcttcaat caaaggatag agcggatggg ccggaattgt gaacgtagaa
      721 acggcgctgc ggctctcgcc gctctcttcc tgaacagaaa cggaaacacc ggtttcatcc
      781 ggttcaatgg aaggagcaag cggctcttcc gttcttcccg aggaaacgaa agcatcatcg
      841 tgtctctccg tctcggggag cgcggtttga tctgtgttct ccgtatcaga ggaacttcct
      901 tcttccgtcg cttccgagac ttttgtttct tgaggttctc cctctgatac ggaagctgga
      961 acataccgcc tggtttctgt tttttcaaca acctctgaaa caacaggaaa catcacgtcc
     1021 tgttgaccag aggactcacc ctggatttca gtcgcttgag ggagagaaga cattgttccg
     1081 tctgcgtcgg atacttcaac atccacggag ccgccggttt gagaaacttg aagaggctct
     1141 ccggcgctcg ctgctgggct acggttttcc gcagtctctt ccggcaggac aacaggaagc
     1201 ccaagaagct ccgccagaga cgcaccgcta tttttgttcg ggtcttcctg tttcaaagga
     1261 ggaaactctt cccccctcaa ccagctttcc caatccatgt tcaaaaccac ccaccctcga
     1321 cgtactgcca tccaaagaag tgtaccaaaa gaaaagcact tcaagcaatg ggaaaaaaac
     1381 ttttctcccg tttttatcga agagaaacca cggcatacaa gaaagccctc ttcccataca
     1441 ggaagagggc ttttgatttc atggtgcgga gggggagact cgaactccca cagccttgcg
     1501 gccacaaggt ccttagcctt gcgcgtctac ctgttccgcc acccccgcgc aacgacggat
     1561 attataagcg ggctttttcc caaagtcaag agaggaaaac taaaattctc ttctctcttt
     1621 gattcgggcg gactttccgc ttctgtcacg cagatagtac agcttggcgc ggcggacctt
     1681 tccaaggcgc ttcacttcaa tctggctgat cgaagggcaa tgcagcggga agatacgttc
     1741 cactccgact ccgctcgaga tcttgcggac ggtgaatgtc tcgctgagac cgccgtgttt
     1801 acgggcaata acaacgcctt caaatacctg aatgcgctct ctggaacctt ccttaacctt
     1861 cacatgaacc cgaacagtgt ccccggaacg aaactccggg acatcgctct ttaaaaactt
     1921 tttctcgaca agatttaaca cgttcatttc aatgaaccct ccttgagctt tcacaaaact
     1981 cgattatctt tacacaatct caagcagaca tccggcgaaa acacaactcc ctgaacaaaa
     2041 taaagtccga caggcctcaa gtgatcgttc ttgcaccgag catggaactt gttgatttcc
     2101 ttctagcgcc atccaaaaaa ccgatccaga acggcgcctg cggcggttct cacggaaaga
     2161 tgattatact ctccccctcc gccggaaaga ggcgacagta ccatcgaaca ctctgctaaa
     2221 acttcctcat ggagccctga acctgtacca aagagcaaaa gaattggccg tttctcacgg
     2281 agcatccgtc ttttcgcctc aatccagtgg aatgcgccgg gatgctttct ggcggttgtc
     2341 ccgacgatga gaggaatctc tttctctctc ttcaaaatcc actcgacgct tttcgctacc
     2401 gagggaaaga gtttgagcaa acgaaacgct tcggctcgtt caggattgac gtttgccccg
     2461 ccgccttccg tccagtgacg agtcatcgtc ttcaccaaat cacgctggct cgccaacggc
     2521 gtggttacga agaaacggtc gatcccgtac gttttgcacg aacgggcaat atccgaaaga
     2581 tcaagcccgg taaccgcagc ggtgctcttc tctcctcttc tgtcaagaac gggaaagtgg
     2641 acaagctcca catatattcc ctttgggaga taggggcgga tattcgcacg ggaaataaga
     2701 tcaggacggc gtgcgagagt acgttcaacg gcggcgtttc tccgccagtt ctcaatggct
     2761 gcggcatcgc cggagagcaa ttcctccgga acgcattctc cattccattc cgcagggcgc
     2821 gtacaatgtg ggtgatccag cattcctctg aaaaatgaat cctcgactac tgccgtttct
     2881 ttccccacaa cgccgggaac aaggcgggcc attgcgtcga tgatcgccat cgccgggatc
     2941 tctcccccgg tcaggacaaa atcacccata gagacctccc tgtcgacaca tcgtgagacg
     3001 aagcgttcgt ccagcccctc gtaatgaccg cagaaaagaa tgatgtgctc ctgtgccgcc
     3061 agggtctcca caatttcctg agaaaagagg tcgccctgag gggatggata gaccacatac
     3121 ccttttgtac ccgaagactc ccgaattgca gcaagagccc tctcaagcac ttccggaaga
     3181 agcaccattc cgccttcgcc gccgaaagcg taatcgtcta tctggcgata gtttccgagt
     3241 ccgaagttcc tgagatccac cacatcgaca tgcagaagac cgtttttgat gcctctgccg
     3301 atcatgcttg tggacagaaa tttttcgaag aactccggga atgctgtaac aatcgttaca
     3361 tgcacgactc ccacagcccc tcgatgagca gaatacgtat ctcttttttt tcaaggttca
     3421 cttcggcgac aaactgtttc accgccggga tatagtggtc atttccggaa tcgtcgcgaa
     3481 ttacataaat gtcgttttcg ccggcagtcg ccacatcgac caattttcca agagacgcgc
     3541 cgtcttcatg ccggacagcc gaaagtccct tcagatcctc tatccagtat tctccctcgc
     3601 cgagcggata ccgttcctcg ggaagaatct cgatgaaagc cccggaaaga ggttctgcat
     3661 cctctattcg acgcagctcc tcggtctgaa tcacaaggag tttcttgtgg tcggagtatc
     3721 ggctggacag aagcgtgagc gtggtgataa tctgtccctc tgcgctatac accgacattt
     3781 tttccattga gtgaaatctc tccggataat cggtcagggg tactatcttg agcgcccctc
     3841 tcagcccgtg agttccgaca atgcgtccga tgcgtactct attcatcctc gacgatatcg
     3901 acgtcaaccc ggtcgttcac tttcactgcg gaagcttttg ctaccaggcg aatcgcgttg
     3961 atcgtcgcgc cgcgtttccc gatgaccctt ccgacatcct ccggagcgac tcgaatggtg
     4021 atcgccgtga cgcccctttc gtccgtctgc tcactgatct gaacctcgtc aggctgtgtc
     4081 acgaggcgtc ggacgatgaa ttcaacaagc tcccggtagt cgggcatgac tactccgcct
     4141 gtttcttgcc cgcttcgaac ttttcccaga ccccggactt tctcaaaatg ctcctggctg
     4201 tgtcggaagc cgtcgcgccg actttcagcc agtgcatcgc ccgctcttcg tccacattga
     4261 ttgaagaggg atctttcatt gggtcgtagg ttccaagaag ttcgatgaag cgaccgtctc
     4321 tgggtgaacg agaatccgcc accaccaacc gataaaaagg agcctttttc cgtccgtgac
     4381 gggcaaggcg aatacgtact gccatttacc atacacctcc tgatattgtt ccggaacaca
     4441 tcgaatacat tgcttccccg gagtgctccg tcaccgggaa cgttctattt aaaacgaaaa
     4501 ccgccgccgc ctccaaggag gcctttcggt agtttgaagc ccttcccctg ttttccgaac
     4561 tgcttccata gcttccgcat ctgctcgtac tgggccagaa gctgatttac catctggacc
     4621 gatgttcccg agcccatcgc aatccgacgt ctgcgactgc ccttgatgat cgaaggatcc
     4681 ctccgttcgg cgcgagtcat cgactgaatg atagccttcg attttttcaa ccgccccgaa
     4741 tcgacttcgt cctgggacac gcctttcatc ttgctgaatc cggggatcat ctcaaggact
     4801 ttgttcaggg gccccatttt ctcaagctgc tcgaactgcg caagaagctc ctcaagtgta
     4861 aatttcttct tcagcgtctc ggccatgcgc tcggccccgc cggaatcggc catctcctga
     4921 attttttccg ccaggccgat gacatcgccc attccgagta ttctctgggc catcctcttc
     4981 gcatcgaaga cttcgagagc gtccgttgcc tctccaacac cggccagctt gaccggcact
     5041 ccggtcacct tgcgaattgc gagggcggcg cctccgcggg cgtctccgtc gagctttgag
     5101 agaacgactc ctgtcagttg gagtttctca tgaaatgaag aggcaacctt taccgcctcc
     5161 tggccggtca tggcatccac aacaagccg
//
LOCUS       DAOPKU010000171         5182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_17388_length_5182_cov_4.466745, whole genome shotgun sequence.
ACCESSION   DAOPKU010000171 DAOPKU010000000
VERSION     DAOPKU010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17388_length_5182_cov_4.466745"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PK849_06605"
     CDS             complement(<1..1178)
                     /locus_tag="PK849_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE65834.1"
                     /translation="MTGVLFGSLVFFFLLNVPIAVAIGLASLSAILWSGAIPPVVLVQ
                     KMFTATDSFPLMAVPFFILAGSLMEFGGISRRLVDFANSIVGRFSGGLAFVAIIASMF
                     FGAISGAAVACVAAIGTILIPAMVRRGYDTPYATAVQATAGTLGVMIPPSIPMIIYGV
                     LTGVSIGALFMGGILPGILVGGSLMLVAWMIARKRGYKGDEKASGAKIWATFKDAILA
                     LLMPVIILGGIYGGVFTPTEAAVVAVAYGFIIGFFVYRELSLSKLKDILVNTSVGTAT
                     IMFIIATSSVFSWILTAQRVPQAVAEAILSISTNPIVILTLINLLLLFIGTFMETVAS
                     IIILVPVLLPVVTQIGVDPLHFGIIIVVNLAIGMVTPPLGVCLFVGCGNMARRMHADR
                     T"
     gene            complement(1175..1675)
                     /locus_tag="PK849_06610"
     CDS             complement(1175..1675)
                     /locus_tag="PK849_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE65835.1"
                     /translation="MSFFRKTLDRVNALAEYAVCALLAVMVVVVFLQVIFRFIIRSSL
                     PWSEELSRYVMVWIVFLGASIGIKRKSHIGVEAVVAMLPDSMRRWTAILVNALCCVFF
                     VFMVHYGRMILRVVSSQLSPAMEISMAIPYSAVFVGGTLMFVHCFFQMVELMVEALSG
                     RAVKSA"
     gene            complement(1783..2772)
                     /locus_tag="PK849_06615"
     CDS             complement(1783..2772)
                     /locus_tag="PK849_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPE65836.1"
                     /translation="MKLKGMVVMALACAMVFSLVTFGEAAKYEFKLGHAVSDQHPYHL
                     GAQKFKEIVEKETNGEIEISLFPNNQLGTGERDLLEGLQLGTVDLYVGSTGPMGGFEK
                     RFMMFDFPFLFKDKAQAYAVLDGKIGQYVMGLLDRIGITGLAWYENGFRHFTNSKRPV
                     NSPEDVKGLKLRTMENKIHMALWRAMGADPTPMAWGEVFTALQQGTVDGQENPVPIIF
                     VQKVYEVQKHLALTGHVFSPAMIAIAKAKFDALPEVHKEIIRKAAQESALFQREQITK
                     MESEQVAKLKELGMEVTTPDVTPFREATKAVYDEFKSELGDDAKLLDEILSGK"
     gene            complement(2819..3751)
                     /locus_tag="PK849_06620"
     CDS             complement(2819..3751)
                     /locus_tag="PK849_06620"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HPE65837.1"
                     /translation="MKTVILGSGAMGSLYGGMLAEAGFDVTLVDVWKEHIDAVNASGL
                     SIEGIGGDRVIRSIRGTTDPAQAGKADLVIVFVKATATAQAMKGAMALLGPETVVLTL
                     QNGLGNVEKLNETAGAERVMAGITGHGCTLLGPGKIRHAGQGDTILGEPRGQISDRLK
                     RVGGMFEKAGFSVKLSENVIGLIWGKLFVNVGINALTAVTGLKNGRLVELQETEELLE
                     LAVREALEVAQAAGIRIETENPVEHTKKVARLTAANRSSMLQDVTNRRQTEIDVINGA
                     IVNEGKKLGVPTPVNLALTNLVRVRQKTYDEFSV"
     gene            complement(3887..4666)
                     /locus_tag="PK849_06625"
     CDS             complement(3887..4666)
                     /locus_tag="PK849_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPE65838.1"
                     /translation="MEGKQRATGGVQSIERAFAIIELLDAQGELGIAEMSRLLSLERS
                     TVHRIVSTIKNLGYINQNPLNHKYSNSFKFFEIGNDVVKRLGLRQQAAPFLRELSEKT
                     HEAVNLAILDGDRVIYIDKIESQSTIKVDLSVGKRFPAYCTGLGKILLAWLPEPKILE
                     IVGPSPFPRYTENTVTSTEDLLAQLREIRDREIAWDNEEYVEGLFCIAAPVRGNSGDV
                     VAAMSVALPKFLYAGESRKIEAVGDLLLDVARRFSRSLGYK"
BASE COUNT         1190 a         1547 c         1348 g         1097 t
ORIGIN      
        1 gtccgatcgg cgtgcattcg ccgggccatg ttgccgcaac cgacgaagag gcagacgccg
       61 agcggagggg tgaccattcc tatggcaagg ttcaccacaa taatgatgcc gaagtggagc
      121 ggatcgacgc ctatctgcgt cactaccgga agaagaacgg ggaccaggat gatgatggag
      181 gcaaccgtct ccatgaaggt gcctatgaag agcaaaagaa ggtttatgag cgtgaggatg
      241 acgatcgggt tcgtcgagat cgagaggata gcctcggcca cagcctgagg tactctctgc
      301 gccgtcagaa tccagctgaa gaccgacgag gtcgcgatga tgaacatgat cgtcgccgtg
      361 ccgaccgaag tgttcaccag gatatccttg agcttcgaga ggctcagttc ccggtatacg
      421 aaaaatccga tgatgaagcc gtaggccacc gcgacaaccg ccgcttccgt cggggtaaag
      481 actcctccat agatgccgcc gagaatgatg accggcatca ggagcgcgag aatcgcatcc
      541 ttgaaggtcg cccatatttt tgcgccgctt gccttttcat cgcccttgta ccccctcttg
      601 cgcgcaatca tccacgcaac gagcatcagg gatcccccga cgaggatacc ggggaggatg
      661 ccgcccatga acaacgcgcc gatggagacg cccgtgagca cgccgtaaat gatcatcgga
      721 atgcttggcg ggatcatgac gccgagcgtt cccgcagtcg cctgcactgc agtggcgtag
      781 ggcgtatcgt atccgcgccg gaccattgcc ggaatgagga tcgttccgat ggcggctacg
      841 caggctactg cggctccgga gatcgcgccg aaaaacatgc ttgcgataat ggcgacgaaa
      901 gcgagtccgc ctgaaaacct gccgacgatt gagtttgcga aatcgaccag gcggcgggag
      961 attcctccga actccatcaa cgacccggca agaatgaaga agggaaccgc catgaggggg
     1021 aaggaatcgg tcgcggtgaa catcttctgc acaagcacca cgggcgggat ggcgccggac
     1081 cagaggatcg ccgagagcga ggccaatcca atggccaccg caatcggaac gttcaacaga
     1141 aagaaaaaga caagacttcc gaagagaact ccggtcatgc gctcttcacc gcccttcccg
     1201 aaagagcttc gaccatcagt tccaccattt gaaagaaaca gtgaacgaac atcaacgttc
     1261 cgccgacaaa aaccgcgcta tagggaatcg ccatcgatat ctccattgca ggagagagct
     1321 gcgaactcac aaccctgaga atcatcctgc cgtaatggac catgaaaacg aagaaaacac
     1381 agcacagcgc gttgacgaga atagccgtcc agcggcgcat cgaatccggc aacatcgcga
     1441 ccacggcctc cacgccgata tgggatttcc gcttgatgcc aatgctcgct cccaggaaaa
     1501 cgatccatac cataacgtat cgggacagct cttccgacca cggaagcgaa gagcgaatga
     1561 tgaaacggaa aataacctga aggaacacca ccacgaccat caccgcgagg agagcgcaca
     1621 ctgcgtattc cgccaaggcg ttcaccctgt cgagtgtttt tctgaaaaag gacataaatg
     1681 cccccctttc gctcagacaa taatctccgg aaaggcccgc cgaaggagga ctgaaggaca
     1741 actctccctt cggcggaata ccggttcaat accgggagaa gattacttcc cgctcagaat
     1801 ttcgtcgagg agtttcgcgt catcgccaag ctcgctcttg aactcgtcgt agacggcttt
     1861 cgtcgcctca cggaagggag tcacgtcggg cgtcgtcact tccatgccga gctccttcag
     1921 cttggcaacc tgttcgctct ccatcttcgt aatctgctct cgctggaaga gcgccgattc
     1981 ctgcgcagcc ttacggatga tctccttgtg tacttcggga agcgcgtcga acttcgcctt
     2041 cgcgatggcg atcatcgcgg gagagaagac gtgacctgta agcgcaaggt gtttttgcac
     2101 ctcgtacacc ttctggacga aaataatggg caccgggttc tcctgtccgt cgaccgtccc
     2161 ctgctgcagc gcggtaaaaa cctcgcccca cgccatcggc gtcgggtcgg ctcccatggc
     2221 tctccagagc gccatatgga tcttgttctc catggtccgc agcttgagcc ccttcacatc
     2281 ctcgggggag ttcacaggcc gtttggagtt ggtgaaatgg cggaacccgt tctcgtacca
     2341 cgcaagaccg gtgatgccga tcctgtcgag gagccccatg acgtactgcc cgatcttccc
     2401 gtcgaggacg gcgtatgcct gcgccttgtc cttgaagagg aacgggaagt caaacatcat
     2461 gaagcgcttc tcgaagccgc ccatcggccc tgtcgatcct acatagaggt cgaccgttcc
     2521 gagctgaagc ccttccagca gatcccgctc ccccgtgcca agctggttgt tcgggaaaag
     2581 ggagatctcg atctcgccgt tggtctcctt ctcgacaatc tccttgaatt tctgcgctcc
     2641 caaatgatac ggatgctgat cgcttacggc gtgtccgagc ttgaactcgt acttcgccgc
     2701 ctccccgaac gtcacgagcg agaaaaccat cgcacacgcc aacgccatca caaccatgcc
     2761 tttcaacttc atattccttc ccctccttac gtgaattgga cgtctccccc gcggtaagtt
     2821 acacggaaaa ttcgtcatag gtcttctgac ggacccttac gaggttcgtc agcgccaagt
     2881 tgacgggagt gggcaccccc agctttttcc cttcgttcac gatcgcgccg ttgatcacgt
     2941 cgatctccgt ctgcctccgg tttgttacgt cctgcagcat cgaggaacgg ttcgccgccg
     3001 tcaggcgcgc cacctttttt gtgtgctcga cggggttctc cgtctcaatg cgtatccccg
     3061 ctgcttgcgc tacttccaac gcctcccgaa cagcaagctc cagcagctct tcggtttcct
     3121 gaagctcgac gagtctgccg ttcttgaggc cggtcacggc ggtcaaggcg ttgattccga
     3181 cattcacaaa cagcttcccc caaatgagtc cgatgacgtt ctcggagagt ttcacggaaa
     3241 aacccgcttt ttcaaacatc ccgccgactc tctttaacct gtcgcttatc tgcccgcgag
     3301 gctcgccaag gatcgtatcc ccctgccccg catggcgtat ctttccggga ccgagaagcg
     3361 tacatccatg ccccgtgatt ccggccataa cccgctcggc tcccgctgtt tcgttcagtt
     3421 tctccacgtt accgagaccg ttctggagcg taagcacaac ggtctccggc ccgagaagcg
     3481 ccatggcgcc cttcatcgcc tgcgctgtag cggtcgcttt tacgaagacg atgacgagat
     3541 cggctttccc cgcctgcgca ggatcggtcg tgccccgaat ggagcggata acgcggtcgc
     3601 cgccgatgcc ttctatcgaa agccccgatg cgttcaccgc gtcgatatgc tccttccaga
     3661 cgtcgacaag cgttacgtcg aatcccgctt cggcgagcat gccgccgtaa agagaaccca
     3721 ttgcgcctga acccaggata acggtcttca ttttctcctc ctacttgaat tcgttcttct
     3781 tccctagagg acggagtgtg cgatacgcac atccttccga attgcctctc taaccgggcg
     3841 gacagctcca taaaaaaacc gtctcttatg aggacgacgt cgtagattat ttatacccaa
     3901 gtgaacggga gaatcttctc gcgacatcca gcaacaaatc tcctaccgct tcaatcttac
     3961 gggattctcc agcgtagagg aatttcggaa gcgcaacgct catcgcggcc acgacatccc
     4021 cggaattacc ccgtaccggc gcggcgatac agaagagccc ctcgacatac tcctcgttgt
     4081 cccaggctat ctcgcgatcc ctgatctccc ggagctgagc gagcagatct tcagtcgatg
     4141 tcacggtgtt ctccgtatac cttgggaagg gagatgggcc tacaatctca agaatcttcg
     4201 gctccggaag ccacgcgaga aggatctttc caagtcccgt acagtacgca gggaagcgct
     4261 tccccacaga aagatccacc ttgatggtag attggctttc tatcttgtcg atgtagatca
     4321 ccctgtcgcc gtcgagtatg gcgagattca ccgcctcgtg cgtcttctcg gaaagttccc
     4381 ggagaaaggg agcggcctgc tggcgaagac cgagccgttt gacaacatcg ttgccgatct
     4441 cgaagaattt aaagctgttc gaatacttgt gattgagggg gttctgattg atgtagccga
     4501 gatttttgat ggtggacaca atacgatgga ccgtactgcg ctccaaggaa agcagacggc
     4561 tcatttctgc aattccaagc tctccctgag cgtccagaag ctcgataatc gcgaaagcgc
     4621 gttctatgga ttggactccg cccgtggccc tctgcttccc ttccatactt tttcctccat
     4681 accatccgag agaatctcgc gtttcatttc ccgcattacg gaatgatatt gattatagtg
     4741 gaattgatct tgcggatcat tataataacc ggttctttct catgtcaata gaaaaagagc
     4801 gcaattccgc caagacagta agttcttttt ttatcaatta tgacgcggca aaagggagaa
     4861 gagcgttgcg acggatacta cgaaagggaa aaagacagat ccggtttgga aaatatggga
     4921 cgggggcgac acgcaacaac aaccgccgtt ttgacgttcg aaaggaaaag gaccgcgtca
     4981 gcacaaagcg cggtccttta aaacccgacg aatccgaaac gattagttcc cgccgatatg
     5041 cgccgatact tcgcgcaaga ctccggtccg caggataccg ccttcctcct ggatggcctc
     5101 catttcgtcg cgcaactcat cgacgtactc atcgtcctta atcgaaggaa ggttaatcaa
     5161 gacgttcata gcggcgcttt cg
//
LOCUS       DAOPKU010000172         5137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_17565_length_5137_cov_4.378394, whole genome shotgun sequence.
ACCESSION   DAOPKU010000172 DAOPKU010000000
VERSION     DAOPKU010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17565_length_5137_cov_4.378394"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK849_06630"
     CDS             complement(<1..282)
                     /locus_tag="PK849_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE65839.1"
                     /translation="MSHDYDVLVVGGGVVGCAIARELSRFKVSAAVLEKEMDVGLGTS
                     CRNSGVLHAGFNYAPGSLRAVLDVKGNAKMDELCRDLKVKIKRIGKLTVA"
     gene            470..1228
                     /locus_tag="PK849_06635"
     CDS             470..1228
                     /locus_tag="PK849_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPE65840.1"
                     /translation="MNKTRKELIHRLSDLITEGDIVSEGKLPPERELAAILGTTRPLL
                     REALIALEALGVLDIRDRQGIFLAEEKVGEIGRVLGQAQVWPLELLSQVMEIRQLLDP
                     GAAALAALRRKERDIAKMDECIFLLEKLHADRDPQEALLGAYWNTVLHATIFKATGNT
                     LLSRLYESLLEMSEKGISAMRMEVLDSAAPERTEQILEQHRLLVSAIKEQDVKTAREA
                     SKKHLKFTIDTLVELSRVSPVSNFFAERMDSALE"
     gene            complement(1309..3435)
                     /locus_tag="PK849_06640"
     CDS             complement(1309..3435)
                     /locus_tag="PK849_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE65841.1"
                     /translation="MLKNLKIGVKLAGGFSLLLVIFAAAGWFAWKNMNEVIAETGTLE
                     ERYLPGIHLSNEVENLGQAVMLNVRTYVLGESFADLDAAKKNFEALLAVLARGKEHAS
                     KHPGLEQLAEGMNTAEEQVTLYQQLLVESQGHMANIATQRRHAAEAAASMGAVAYDLL
                     FDEKDELEKETEEGAGADTLLDRQESILLLDDLVEAVHKAESYTLRSIAERRIELLDR
                     VEDELKRVESLLKKVQATAVKQRQKDQLKKGFEDVESYKGAIASFREAWKDLDEMGKT
                     RTAAAEGVLAAAAKVMNFSVERTLQIAGVVTGQARDVSGMLIASVLFALFAGIVVAFL
                     ITRMITRPLHRAVALAERAGGGDLTIERKDFGHEGKDEIGILADALAMMVKNQADSVR
                     NVVAAAHEISQGAGALAALSQETNASVEEVRSSLEQVASISENNSAALEESNAGVEEV
                     AAGAQASAKASSEGVAAAEHTAAVAKEAVDRVADVIEDVHSVGGKSQESVEKIRALAA
                     SVENISRFVSVISSIADQTNLLALNAAIEAARAGDAGRGFAVVADEVRKLAEESNRAA
                     REVAAIISGLDESARESIVATEEAERIMSRTAVKADDVRQRLAGVLTEIGNISGVISH
                     IASVAQNQAASSEEMASAIDQVSHSTIDVVQRVDAIRSASGEMAGASEGVAREASEMA
                     ERAERMQQLLARFILSRDEQGLAPID"
     gene            complement(3685..4791)
                     /locus_tag="PK849_06645"
     CDS             complement(3685..4791)
                     /locus_tag="PK849_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE65842.1"
                     /translation="MKVRDFLLERYFAKYEFSVRHMLSASDCETISVRELLEGADADV
                     EELWRELRLSYTESRRHPALLREIARETGVSADEAIVLNPEDGIFLTLNALLDSDDHV
                     VVLSPCYQSLYELPKSLGCEVTRWYLKKEDSVWKLDLPFLERAIKENTRLVIVNFPHN
                     PTGFLPSVEQLNAIAELSVKKGFFVFSDEMYRGLEHNPSDRLPSAPSLIRRSVALGGL
                     SKAYGLPGLRTGWVTSTDKWLLDRIAALKDYTTICTSAPSEILAIAALRKSDELTGRC
                     RGIVKENLEMARSFFQENSGRFEWFEPHGSSVAFPRLLGGQPVKDFCERAVTERSLLV
                     LPDVVFDVDWNHFRIGLGRRSFPEALSVLREMLN"
     gene            complement(4929..>5137)
                     /locus_tag="PK849_06650"
     CDS             complement(4929..>5137)
                     /locus_tag="PK849_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65843.1"
                     /translation="AGMDSVSKSSIEIAEQVGSVSRSMQEQTKVVESIAASSEELVSL
                     SASMQQAVSRFKVSEESRGLALKK"
BASE COUNT         1054 a         1647 c         1379 g         1057 t
ORIGIN      
        1 ggcaaccgtc agcttgccga tacgcttgat cttcaccttg agatcgcggc agagctcatc
       61 catctttgcg tttcccttca cgtcgagaac cgcacgcagc gatcccggcg catagttgaa
      121 tcccgcgtgg agcacgccgc tgttccggca gctcgtaccg agtccgacgt ccatctcctt
      181 ctccaggact gcggcgctca ccttgaagcg cgacagctcc cgggcgatgg cgcaccctac
      241 tactccaccg ccgacgacaa gtacatcgta gtcatgcgac aacctgcaac acctgctttc
      301 aaagaaaaag acgttttttt cgcatactgg tcgaccaagg tattccaatt atccggcgac
      361 tatagtatca tagcctccaa agcaagacaa gataaatgca ctgaaaattt caagttcttt
      421 ttttaacaaa tcgatagtgt catgtcgata tctgcttggg gaggcctcca tgaacaagac
      481 tcgaaaagaa ctgatccaca ggttatcaga tttgatcacg gagggggata tcgtttccga
      541 gggcaagctc ccccccgaaa gggagcttgc cgccattctg ggaacgacac gaccgcttct
      601 ccgggaggcg ctcatcgctc tcgaagcgct gggcgttctg gatatccgcg accggcaggg
      661 tatttttctc gccgaagaga aggtcggcga gatcgggcgc gtcctggggc aggcccaggt
      721 gtggcccctc gagctgctct cccaggtgat ggagatccgc cagctcctcg atccgggggc
      781 ggccgcgctc gccgcgctcc ggcgcaagga gcgcgacatc gccaagatgg acgagtgcat
      841 ctttcttctc gaaaagctcc acgccgaccg cgatccgcag gaggctttgc ttggagccta
      901 ctggaacacg gtgctccacg cgaccatctt caaggcgacg gggaacaccc ttctctcacg
      961 gctctacgag agcctgctcg aaatgtcgga aaagggaatc tcggcaatgc ggatggaggt
     1021 gctcgactca gccgcgcctg agcggacgga acagatcctt gagcagcacc gcctgctcgt
     1081 ctccgccata aaggagcagg atgtgaaaac agccagagag gcctcgaaga agcatctcaa
     1141 gtttactatc gacacgctgg tggaactgag cagggtctct cctgtttcga acttttttgc
     1201 cgagcggatg gattccgccc tggagtgacg acacggaaac agtgacacaa acacaaagaa
     1261 ggagcccttc accgagaggg ctccttcttt gtatgctctc tttctgttct agtcgatcgg
     1321 agcaagcccc tgctcgtccc gggagagaat gaagcgtgcg aggagctgct gcatcctctc
     1381 ggcgcgctcc gccatttcgg aagcctcgcg ggcgacgcct tcggatgcgc ccgccatctc
     1441 gccggatgcg ctccgtatcg catccacccg ctgcacgacg tcgatggtcg agtgcgagac
     1501 ctgatctatc gcgctcgcca tctcctcgct tgacgcggct tgattctgcg ccacggaggc
     1561 gatgtgcgag atcacaccgg agatattgcc gatctcggta agaacgccgg cgagacgctg
     1621 cctgacgtcg tccgctttca cggctgtccg ggacatgatg cgctccgcct cctcggtagc
     1681 gacgatggat tcgcgcgcgc tttcgtcaag cccggagatg attgcggcca cttcgcgcgc
     1741 agccctgttc gactcctccg caagcttcct cacttcgtcg gcgacgacgg cgaacccccg
     1801 cccggcatct cctgccctgg cggcttcgat agcggcgttc agcgcgagaa gattcgtctg
     1861 atccgctatg gagctgatga cggagacaaa gcgagagata ttctccaccg aagccgcgag
     1921 ggcgcggatc ttctccacac tctcctgcga cttgccgccg accgagtgga cgtcttcgat
     1981 gacgtcggcc acccggtcca ccgcctcctt tgcaaccgcc gccgtatgtt ccgccgcggc
     2041 aacgccctcg gaagaggcct tcgcggacgc ctgcgccccg gcggcaacct cttcgacccc
     2101 ggcgttcgat tcctcaagcg cggcagagtt gttttcggag atcgaggcaa cctgctccag
     2161 cgaactgcgg acttcctcca cggatgcgtt cgtctcctga gacagagcgg ccagggcgcc
     2221 ggcgccctgg gaaatttcat gggcggcggc gaccacattg cggaccgaat ccgcctggtt
     2281 tttcaccatc atggcaaggg cgtcggccag tatgccgatt tcatccttcc cttcatgacc
     2341 gaagtccttc cgctctatgg taagatcccc gccgcctgct ctctccgcaa gcgcgaccgc
     2401 cctgtgtaga ggacgggtaa tcatgcgcgt gataaggaac gcgacgacaa tgcccgcaaa
     2461 gagcgcaaaa agcacggaag caatcagcat cccggagaca tcgcgcgcct gtccggtcac
     2521 gactccggcg atctgcagcg tgcgctccac gctgaagttc atcaccttgg ccgccgctgc
     2581 cagcactccc tcggctgcgg ccgtcctggt ctttcccatc tcgtccagat ccttccatgc
     2641 ttccctgaag gacgcgatgg cgcccttgta cgactccacg tcttcaaagc cctttttcag
     2701 ctggtctttc tgcctctgct tgacggctgt cgcctgcacc ttcttcagca acgactcgac
     2761 ccgcttcaat tcgtcttcaa ccctatccaa cagctcgatc cgccgttcgg cgatagagcg
     2821 gagcgtatag ctctccgctt tgtgcaccgc ctcgacaagg tcgtccagga gcaagatact
     2881 ctcctggcga tccagcagcg tgtcggcgcc ggctccctcc tcggtctcct tctccagttc
     2941 gtccttctcg tcaaaaagga gatcgtaggc gacggccccc atggaagcgg ctgcttccgc
     3001 cgcatgcctg cgctgcgtgg cgatgttcgc catatgcccc tgcgactcca caaggagctg
     3061 ctgatagagc gtgacctgct cctcggctgt attcatccct tcggcaagct gttccagccc
     3121 ggggtgcttc gaagcgtgct ccttaccacg agcgagcacg gcaagaaggg cctcgaagtt
     3181 cttcttcgcg gcgtcaagat cggcaaaact ctctccgagg acataggttc ggacgttcag
     3241 catcaccgcc tgcccgagat tctccacttc gttggaaaga tgaattccgg gaagatagcg
     3301 ctcctcaagc gtacccgtct ccgcgatgac ctcgttcatg ttcttccagg caaaccatcc
     3361 ggccgcggca aaaatgacca gaaggaggga aaaacctccc gcgagcttca cgccgatctt
     3421 taaatttttc agcaaaaccc tcacctcttc ttgaatggaa taacaaccga acttccctcc
     3481 gctgcaaacg cccgcaatgc gaccgcccgt tactatagca taaagaggga tatcaaaaaa
     3541 acgccgaaag tcttcggcgt tgttctttgc ttcacgttaa aactcgtccg ttatttttta
     3601 caattatgca tcgtcccatg ctacgatcgg aagacaacta tcggaagaaa gcccctcgct
     3661 caaggccggg ggctttggga aaaatcagtt gagcatctcg cgcagaacag agagagcctc
     3721 cgggaacgag cgtctcccca ggcctatcct gaaatggttc caatcaacgt cgaagacgac
     3781 gtcgggcaat accaacagac tacgctcggt cacggcgcgt tcgcagaaat ccttcaccgg
     3841 ttgtccgccg agaaggcggg ggaaggcgac cgagcttccg tgaggttcga accactcgaa
     3901 ccgtccgctg ttttcctgga aaaaggagcg cgccatctca agattctcct ttacgatgcc
     3961 gcggcagcgg ccggtaagct catcgctctt gcgaagagcg gcgatcgcga ggatttcaga
     4021 gggcgccgaa gtgcagatcg tggtgtagtc cttcaaggcg gcgatcctgt ccagaagcca
     4081 cttgtccgtc gacgtaaccc atcctgtccg cagccccggc agaccgtacg ccttggagag
     4141 accgcccaaa gcgacgcttc tgcggatgag cgacggcgcg gagggaagcc gatccgaagg
     4201 gttgtgctca agcccccggt acatctcgtc ggagaagacg aagaatccct tcttcaccga
     4261 aagttccgcg atggcgttca gctgctccac ggacggaaga aaaccggtcg ggttatgcgg
     4321 gaagttgacg atgacaaggc gcgtattctc cttgatcgcc ctctcaagaa aggggagatc
     4381 cagcttccat acactgtcct ccttcttgag gtaccatcgg gtcacttcgc accccaacga
     4441 tttaggaagt tcgtagagcg actgatagca cggactgagc acgacgacat ggtcgtcgct
     4501 gtccagcagc gcgttgagcg tcaggaaaat cccatcttca ggattgagga cgatcgcctc
     4561 gtcggccgac actcccgttt cgcgggcaat ctcgcggagg agcgccggat gcctcctcga
     4621 ctccgtgtac gaaagacgaa gctcgcgcca gagttcctct acgtcggcat cggccccctc
     4681 aagaagctcg cggacgctta tcgtctcgca gtcggacgcg ctgagcatat gacgtaccga
     4741 gaattcgtat ttggcgaaat atcgttcaag gagaaaatcc cgaactttca ttgcatcaca
     4801 gccttaaaag agattttata tagacgtttt cttttgtatt atacccgcca tcacgccctt
     4861 ccggaagaga ggaacctata aaaaaggacg cccgaaggcg tcctttttgc tcttctcctc
     4921 tgatccgcct acttcttcag cgcgagcccc cggctctcct cgctcacctt gaagcgcgac
     4981 accgcctgct gcatcgacgc gctcaggctc accagctcct ccgacgacgc cgcgatcgac
     5041 tccacgacct tcgtctgctc ctgcatggaa cggctcacgc tccccacctg ctccgcgatc
     5101 tcgatgctcg acttcgacac gctgtccatt cccgccg
//
LOCUS       DAOPKU010000173         5121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_17627_length_5121_cov_4.495065, whole genome shotgun sequence.
ACCESSION   DAOPKU010000173 DAOPKU010000000
VERSION     DAOPKU010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17627_length_5121_cov_4.495065"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..594)
                     /locus_tag="PK849_06655"
     CDS             complement(31..594)
                     /locus_tag="PK849_06655"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE65844.1"
                     /translation="MGISMLFAFLLLMILGVPLYLSLLSTSLFGIVMLGDFSLLRVMS
                     QQFFGGMDVFSLMAIPFFILAGILMNKTGLTDRLLDFSRLLVGSVRGGLGYVNVVGGI
                     ILAGVNGSAAADASALGSILIPAMVKDGFSPPYAAGLTAGSALIGPIIPPSIFMIIYA
                     TMTNTSIGGLFAAGILPGILLGGAFWE"
     gene            complement(599..1117)
                     /locus_tag="PK849_06660"
     CDS             complement(599..1117)
                     /locus_tag="PK849_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE65845.1"
                     /translation="MVFSTLSPESHPSEARESALTVWTHRLERISAWGACLLLAFNIG
                     DILVGVFCRYVLKSSIIWTEEAARFSLVWMVMVGALGAAEKGDHMVIDFVVPRFPAAL
                     RRLIGWGRFLLTILILSLMIYLGTVNAVQMWNMRTMALNIPKTIPLLSLPVGFLLLMA
                     GTILMQFDRGRE"
     gene            complement(1127..2170)
                     /gene="dctP"
                     /locus_tag="PK849_06665"
     CDS             complement(1127..2170)
                     /gene="dctP"
                     /locus_tag="PK849_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE65846.1"
                     /translation="MKFSKVSALFVLFALFAVMVPGYAEAAVEVKMSYNGPGDVENNA
                     VHLYAVNFKKFVEEGTGGEVTIALFPDSQLGNEEERMELLSKQGMNQPIVNVASFAGV
                     APVFPEIYATAVPFMFDGFKAAHIFHDESRFWKKAQEEFHKRTGAYLLEAVEEGGFLA
                     FSNNKREIRKAEDFAGLKFRGMDEGQVILCKSFGASGTPIPWTELYMALKTGVVDGQM
                     NPAMYIKMGSLQEVQKYLTLANIQYSDQFLVVNGDFMDSLAEEHKKAVVEAAKKANDL
                     NRKAMAEQDQKDIDFLVEKGMIAYSPTEEEMETFRSAAQPAYVEWLKSKAGEDWLTLA
                     LECAKNANEMAKK"
     gene            complement(2335..4794)
                     /locus_tag="PK849_06670"
     CDS             complement(2335..4794)
                     /locus_tag="PK849_06670"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HPE65847.1"
                     /translation="MPCSICLHGHFYQPPRENPWLEEVEIQESAAPWHDWNERISEEC
                     YGPNSAARIFDDAGWIRRIVNNYSRISFNFGPTLLSWMERKRPQYYQAVLEADRLGSA
                     RFSGHGPAIAQAYNHMILPLANRRDKITQIVWGIEDFQKRFQRAPEGMWLPECAVDSE
                     TLDILAEHGIRFVILAPRQAQAVREKGASSWTDVTGERVDTHRAYICPLRSGRALSLF
                     FYQGELAQKVAFGDALRNGGDFAKMLIGALPESATLGRLVSVATDGETFGHHHKYGDM
                     ALAYCLDIIERSRSASLTIFGEYLDSTPPVDEVRIVENSSWSCVHGVGRWKDDCGCSA
                     HSRPGWNQRWRGPLREALDTLRDAAAPLFEKEAGMFFPDPWGARNRYIEVFFDHTRSE
                     ATSFLQREAGRALSREEQVRALRLMELQRNLMLMYTSCGWFFDEISGIETLQVMAYAA
                     RALELCWRLFPREDFESPFKNLLSKAPSNIVEFVNGKRVYDIFVTQMRSDLLRAGAHY
                     AVSALFSGDEGDVRANLGDVFYAYRVLSCSLERKDEGARRYALGYLRIRSEITRDEKE
                     LFVAALYRGGRNVLCGVAEKPEPEDGNGIREKIEEALRSEDEEGLVSFFGHNTYSLRH
                     LFKDEQRRILNLIIAEDVEAVVSMLRKTISDSSELLSFLSALAMPIPEAFRSAAEVTL
                     NDELKRAFEADALDLDFVSQRIDEGERWGAGVDVLFLRHAAMRRLERLFLLLEREPFE
                     ENTLRSIIELLSFLKKRGWEINLWDEQNTFAGIIASNEDLSSCGLLYETLGKMLLVRH
                     SRVESDFADRVTPRSRQKGVV"
     gene            4882..>5121
                     /locus_tag="PK849_06675"
     CDS             4882..>5121
                     /locus_tag="PK849_06675"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPE65848.1"
                     /translation="MFFLTRSCGILLPVSSLPGRWGIGDLGPGARSFVDFLSRSGQKT
                     WQILPLSETDGALGNSPYSSPSAFAGNHLFISPDDL"
BASE COUNT         1211 a         1516 c         1348 g         1046 t
ORIGIN      
        1 gaacctggta ccggtgagag tagacgtagt tcattcccaa aaagctcctc ccagcaggat
       61 accgggcagg ataccggcgg caaacagccc gcctatcgac gtattcgtca tggtagcgta
      121 gataatcata aaaatactcg gagggataat cggaccgatg agcgcgctcc ccgccgtcag
      181 ccccgccgcg tagggaggag agaaaccgtc tttgaccatg gcgggaatca gaatggaacc
      241 gagggcggac gcatccgcgg cggcagaacc gttcaccccg gcgaggatga tgccgccgac
      301 gacgttgacg taccccaaac cgccccgaac cgacccgacg agcagacggc tgaaatcaag
      361 cagtctatcc gtcaaccccg tcttgttcat taaaataccg gcaaggatga agaacgggat
      421 agccattaag gagaacacgt ccataccgcc gaaaaactgc tgactcatga cacgcaataa
      481 agaaaaatcg ccaagcatca ctatcccgaa caaagaggtt gagagcagcg agagatagag
      541 cggcacgcca aggatcatca ggagtaaaaa agcgaaaagc atcgatatcc ccataggatc
      601 actccctccc tcggtcgaac tgcatcagta ttgtacccgc catcaagaga agaaacccta
      661 caggcagtga aagaagcggg atcgtctttg gtatattgag cgccatcgtt cgcatgttcc
      721 acatctgcac agcattgacc gtgccaagat agatcatcag tgaaaggatg agtattgtca
      781 ggagaaagcg cccccagccg ataagccgcc ggagggcggc gggaaaacgc ggaaccacaa
      841 aatcgatgac catgtgatcc cctttctccg ccgccccgag ggctcccacc atgaccatcc
      901 acaccagaga gaagcgcgcg gcctcctccg tccagattat ggagcttttg agcacgtacc
      961 tgcagaacac gccgacgaga atatcgccga tgttgaaggc aagaagaagg catgctcccc
     1021 aagcgcttat ccgctcaagg cgatgcgtcc agacagtcag agcgctctcg cgcgcttcag
     1081 aaggatgcga ttcaggagac agggtggaaa ataccatgac gaaccgttat tttttcgcca
     1141 tctcgttggc gtttttcgcg cactcgaggg ccagcgtgag ccagtcctct cccgccttgg
     1201 acttgagcca ctccacgtac gccggctgcg ccgcgctgcg gaaggtctcc atttcttctt
     1261 ccgtcggcga ataggcgatc atgccctttt cgaccaggaa gtcgatatcc ttctgatcct
     1321 gctcggccat ggccttgcgg ttcaggtcgt tcgctttctt tgcggcctcg acaacagctt
     1381 ttttgtgctc ctcggcaagc gagtccatga aatcgccgtt gactacaagg aactggtcgg
     1441 aatactgtat gttggcgaga gtaaggtatt tctgtacctc ctgaagactt cccatcttga
     1501 tgtacatggc ggggttcatc tgaccgtcca cgacgccggt cttcagcgcc atgtagagct
     1561 ccgtccacgg aataggcgtt ccgctggctc cgaaagattt gcaaagaatg acctgacctt
     1621 cgtccattcc gcggaatttc agccctgcaa aatcctccgc tttccgaatc tcccgcttgt
     1681 tgttggaaaa cgcgaggaaa ccgccctcct caacagcctc aaggaggtac gcgccggtac
     1741 gtttatggaa ctcttcctgg gctttcttcc agaaacggct ctcgtcgtgg aaaatatgcg
     1801 cggccttgaa gccgtcgaac atgaagggaa cagctgtcgc gtagatctcc gggaagacgg
     1861 gagccactcc ggcgaaggac gccacgttga cgatgggctg gttcatcccc tgcttcgaga
     1921 gaagttccat ccgctcctcc tcgttgccca gctggctgtc cgggaagagc gcgatggtaa
     1981 cttcaccgcc ggtgccctct tccacgaatt tcttgaagtt caccgcgtac agatgcaccg
     2041 cgttattctc cacatcgccg gggccgttgt aggacatttt gacctcgaca gccgcctcgg
     2101 cgtaccccgg aaccatgacg gcaaacagcg cgaacagaac gaaaagagcc gataccttac
     2161 tgaacttcat tgcacatacc ctcccttgaa aaatacagaa cacccctccg gcaggacacc
     2221 cggagagcgg aaaacggaga gaccatctct ccgaaccatt aaatacccgg atactattac
     2281 accacaaaaa agcgattcga tgcaggactt tcgtctcatc aagagaaaag cgcgtcacac
     2341 cacccctttc tgccgcgaac ggggagttac cctatccgca aaatcgcttt ccacacggga
     2401 gtgccgaacc aacagcatct ttcccaatgt ctcgtagagc aggccgcagg atgaaagatc
     2461 ctcgttcgac gcgatgattc cggcgaatgt gttctgctca tcccacaaat tgatctccca
     2521 acctcttttt tttaagaaag aaagaagctc tattatgctc cgcaaggtgt tttcttcaaa
     2581 cggctcccgt tccagcagga gaaagaggcg ttcgagacgc cgcatcgcgg cgtgacgcag
     2641 aaaaaggaca tcgactccgg cgccccagcg ctctccttcg tctatccgct gcgacacaaa
     2701 atcaagatca agagcatccg cctcgaaggc gcgcttcagc tcgtcattaa gcgtcacttc
     2761 ggcagcgctc cggaatgcct ctggaatagg cattgcgagc gcggagagaa aggagaggag
     2821 ttccgatgag tccgaaatcg tcttcctgag cattgaaacg acggcctcga catcctcggc
     2881 gatgatcaaa ttcaggatac gcctttgctc gtctttgaag agatggcgca gtgaataggt
     2941 gttgtgcccg aagaaggaaa ccagcccctc ttcatcctcg cttcgaagag cctcctcgat
     3001 cttctcacgg ataccgtttc cgtcttccgg ctccggcttc tcggccacgc cgcacagcac
     3061 gttccgtcct ccgcggtaga gagcggcgac aaaaagctct ttttcatccc gtgtaatttc
     3121 ggaacggatt cgaagatagc cgagagcata ccggcgggct ccttcatctt ttcgttcaag
     3181 ggaacacgac aaaaccctgt aagcatagaa aacgtctcca agattcgcac gcacatcccc
     3241 ctcgtcgcct gaaaagaggg cggagacggc gtaatgcgct ccggcgcgga gcagatccga
     3301 acgcatctgc gtgacaaaaa tatcgtagac gcgttttccg ttgacgaatt caacgatgtt
     3361 gctgggagct tttgatagaa ggttcttgaa aggcgattca aaatcctccc gggggaagag
     3421 acgccagcac agttcaagcg cgcgggcagc gtaggccatg acctggagtg tttcgatacc
     3481 ggagatctcg tcgaagaacc agccgcagct ggtgtacatc aacatcagat ttcgctgaag
     3541 ctccatcaga cgaagggctc tgacttgttc ctcgcgcgag agagctcttc cggcctcccg
     3601 ttgcagaaac gatgtggctt cggaacgggt atggtcgaag aagacctcta tataccggtt
     3661 gcgcgcgccc cacggatcgg gaaaaaacat ccccgcttcc ttttcaaaaa gaggagcggc
     3721 agcgtcccgc agcgtatcca acgcctccct gagaggccct ctccagcgtt gattccatcc
     3781 ggggcgggag tgcgcggaac agccgcaatc atccttccac cttccaacgc cgtgaacgca
     3841 gctccaggag gagttctcga cgatccgcac ctcgtcgacg gggggcgtcg agtcgaggta
     3901 ttcgccgaag atcgtgagcg atgcggacct ggatcgttcg attatgtcga ggcagtaggc
     3961 cagcgccatg tcgccgtact tatgatggtg accgaacgtc tcgccgtccg ttgcaaccga
     4021 gacaaggcgc cccagcgtcg cgctttcggg aagcgcccct atgagcatct tcgcgaaatc
     4081 tcctccgttt ctcagggcgt cgccaaaggc aaccttctgg gcgagctccc cctggtagaa
     4141 aaagagcgaa agcgcacgac cggaacgcag gggacaaatg tatgctcgat gcgtatcgac
     4201 ccgctccccc gtcacgtccg tccatgatga agcgcccttt tcccgaacag cctgcgcctg
     4261 gcggggagcg aggatgacga agcgtatgcc atgctcggca agaatatcaa gcgtctcgga
     4321 atcgacggcg cactcgggca gccacatccc ctcgggcgcc cgctggaagc gcttctggaa
     4381 gtcctcgatc ccccagacga tctgtgtgat cttgtcgcgg cggttcgcaa gaggaaggat
     4441 catatggtta tacgcctggg ctatggcggg accgtgtccc gaaaaacgtg ccgatcccaa
     4501 gcggtccgcc tcgaggacag cctgatagta ctgggggcgt tttctctcca tccatgaaag
     4561 aagcgtcggt ccgaaattga aactgatccg cgaatagtta ttgacgatac gccggatcca
     4621 ccccgcgtcg tcaaaaatac gcgcggcgga gttcggcccg tagcactcct cggaaatacg
     4681 ttcattccag tcatgccagg gagctgcgga ttcttgaatt tccacctctt ctagccaggg
     4741 gttctccctg ggcggctggt aaaagtgacc atgcaaacag atcgaacagg gcaagaaaaa
     4801 ccctcctttc ttccgggata aattataccc tttcttcacc tctcttttcg cttggtgtac
     4861 aataaggagc aaaaaacgga ggtgtttttt ctgacgagat cttgcggtat tctccttccc
     4921 gtttcctctc ttccaggaag gtggggcatc ggcgaccttg gtccgggagc cagatcgttt
     4981 gttgacttcc tctcgcgttc gggccaaaaa acatggcaga tactccccct ctccgaaacc
     5041 gacggagcgc tcggcaactc tccgtacagc tcgccttccg cgtttgccgg aaatcatctt
     5101 ttcatctcac cggacgacct t
//
LOCUS       DAOPKU010000174         5058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_17935_length_5058_cov_4.209674, whole genome shotgun sequence.
ACCESSION   DAOPKU010000174 DAOPKU010000000
VERSION     DAOPKU010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17935_length_5058_cov_4.209674"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..938
                     /locus_tag="PK849_06680"
     CDS             225..938
                     /locus_tag="PK849_06680"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HPE65849.1"
                     /translation="MAGTVESLENPKDTAEHRGTSEILLKGTGYGVRMILPETKTDED
                     LLQEALAFSGEAARLTRGIGVVLDFQGRTISRAFLLRFLSEFVWTGAFRVLSWMSYNA
                     DTLELFRASGFSTGEPTAARSEKKESGGATLLLIQSLRSGQRVEHEGDVVLLGHMNEG
                     AEICASGNVFVRGRLKGVVHAGMNGGEYSISAGQFEARQLRLGGKLCSSLGTEMEWWG
                     KAVVISLENDSLVVRELKL"
     gene            992..1798
                     /gene="minD"
                     /locus_tag="PK849_06685"
     CDS             992..1798
                     /gene="minD"
                     /locus_tag="PK849_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HPE65850.1"
                     /translation="MDSRVIVITSGKGGVGKTTTTSNLSVALARGGHRVVAVDADIGL
                     RNLDVVMGLENRVVYNFIDVLEGNCRLNQALVRDKRVDNLFLLPAAQTRTKDAVSSEQ
                     MEELCNLLRQEFEYILIDSPAGIESGFRNAAVGADEALVIATPEVPAVRDADRIIGLL
                     ESMGKAPIRLIVNRLRAQMVRDGEMLDVSDVLDILAIELIGVVPEDDSVVKSANRGEP
                     LTFSDVSPAALAFQRIAMRLTGKEVPFPKLDEENGRGLFAGIRKLFGMKN"
     gene            1818..2102
                     /gene="minE"
                     /locus_tag="PK849_06690"
     CDS             1818..2102
                     /gene="minE"
                     /locus_tag="PK849_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HPE65851.1"
                     /translation="MDFLKKFFSSSGSKSRAKERLQIVLIHDRADISPEMLDNLRQDM
                     IAVITRYMDIDTSKIEMDFDRADRSVAFVANIPVIRIKRGVAAAREGERG"
     gene            2166..3272
                     /gene="rodA"
                     /locus_tag="PK849_06695"
     CDS             2166..3272
                     /gene="rodA"
                     /locus_tag="PK849_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPE65852.1"
                     /translation="MEEKKLSLGESVAGLDKAQLFSVGALFLLGVLSIFSAGAGFEGR
                     GAGFATRQLLWGAVSIAAFLLTLKIGYEKFLREAYLLYGLSLLLLLSVMALGLTVKGS
                     QRWIDLGFFRLQPTEFVKITLVLVLAKHLCRYPPLNFLSFLGGLAVPALSGILVLIQP
                     DAGSAIVYGVISFVAIVVAGAPWRYPAGLLAIFTSLLPFAWRFLREYQKMRIRVFLDP
                     WIDPLGAGYNVIQSRIAVGSGGLFGAGYMEGLQSKLKFLPEPHTDFIFGVFSEEFGFI
                     GALGMLALFGLLFWRLLDAGLRTKDGRAKVLIAGISTWFWFQMLQSIGMSMGLLPITG
                     LPLPLVSYGGSSLLSFSVALGLAQSVYVSTLKRY"
     gene            3362..>5058
                     /locus_tag="PK849_06700"
     CDS             3362..>5058
                     /locus_tag="PK849_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPE65853.1"
                     /translation="MKCRNESAAPEQRAVPREEAVQPALFEYGDGMWPLLSNVKRSSR
                     YAGCEYGVIPPKDGNSIVRFCLAFPDVYEIGMSYLGFQILYFLLKNLPCGDADRVYCP
                     WTDLEEQMRARETPLTSLESGRPLREFDVVGFTLQYELSYTNILTMLDLGRIPLLSAD
                     RGENAPLVAAGGVGALSPEPLAPFIDFFCVGDGEELLPEVITLLSGAKGLSRREKLEL
                     LSGIEGVYVPSLVECDVGEGGVSFRSAAEKRVALPVRRRIVESLDDAFFPEMLLVPSG
                     GIVHDRVAVELFRGCTRGCRFCQAGMVTRPVRERSVRPLAERIVSLVEKTGWEEVGLL
                     SLASCDYSGIGELVRELSPMLAEKNVKLSLPSLRMDAFSVNLAAEMESMRRGGITFAP
                     EAGTQRLRDVINKGVSEEDFENSLRTAFTKGWDRVKLYFMMGLPTETDEDLDGIVRLT
                     ERAYSIGKGIKKRVEVAVSVAGFVPKPHTPFQWEAQLSVDELRRRGRYVKNRLNEVGR
                     GKISLKYHEPEQSFMEGVFARGDRRLAPVLLAAWRKGARFDGWTETFSFSRWMEAFE"
BASE COUNT          947 a         1400 c         1638 g         1073 t
ORIGIN      
        1 cgaaatatgt cgcggtggcc atgctggagg ccgggctcca cggaggttcc gtggcctcgc
       61 ccatggtggg ggagatgctc tcgtatctcc tcgttcccga atccaggatg aaaactccgt
      121 aaaggagatg atggaagccg cccggggagt tcggtatttc ccgccgaaca taccgcagag
      181 gtggaaaatg gagaagcgag ctttttaccg aaggaggggg tactgtggct ggaacagtgg
      241 aatctcttga aaatccgaag gatactgccg aacaccgcgg gacgtccgag attctcctga
      301 aaggaacggg gtacggagtc cgaatgatac tcccggagac gaagacggac gaagatcttc
      361 tgcaagaggc gcttgccttc tcgggagagg ctgcccgtct gacccgcggc atcggcgttg
      421 ttctcgactt tcaggggaga acgatctccc gggctttcct gctccgtttc ctttcggaat
      481 ttgtctggac gggggctttc cgcgttcttt cgtggatgtc ctacaatgcg gatacgcttg
      541 aactctttcg cgcatccggt ttttccaccg gcgaaccgac tgccgcgagg agtgaaaaaa
      601 aggagagcgg aggcgccacg cttctcctca tacagtcttt gcgttccgga cagagagtgg
      661 agcacgaggg cgacgtggtg ctcctcggac atatgaacga aggggcggag atatgcgcct
      721 cgggaaacgt ctttgtcagg ggcaggctca agggcgtcgt ccacgccggg atgaacggcg
      781 gcgagtacag tatttcggcg ggccagttcg aagccaggca gctgcgcctc ggagggaagc
      841 tctgctcctc cctcggcacg gaaatggaat ggtggggaaa ggccgtcgtg atcagtctgg
      901 aaaacgattc tctcgtcgtg agggaactga agttataagc ccccgaatat cgctctgaaa
      961 gggcgatcgg gagggaagga gagatcttgc tgtggattct cgtgttatcg tgattacctc
     1021 cggaaaggga ggggtgggaa agacaaccac tacatcgaat ctttcggtgg cgctcgcgcg
     1081 cggcggtcac cgcgtcgtag ctgtcgacgc cgatatcggg ctcaggaacc tcgacgtggt
     1141 gatggggctc gagaacaggg tggtctacaa cttcatcgac gttctcgagg ggaactgcag
     1201 gttgaaccag gcgctcgtca gagacaagcg cgtggacaac ctttttctgc tccccgccgc
     1261 gcagacccgg acaaaagacg ccgtctcctc cgaacagatg gaggagctgt gcaaccttct
     1321 ccggcaggag ttcgagtata tcttgatcga cagtccggcg gggatcgaga gcggtttccg
     1381 gaacgctgcc gtgggcgcgg acgaggcgct tgttatcgcc acgccggagg tgccggctgt
     1441 gcgtgacgcc gacaggatca tcggccttct tgaatcgatg ggaaaagcgc cgatccgtct
     1501 gatcgtcaac cgcctccgcg cgcagatggt ccgcgacggc gagatgctcg acgtttccga
     1561 cgtactcgac atactcgcga tcgagcttat cggtgttgtt cccgaggacg acagcgtcgt
     1621 gaagtcggcc aaccgcggcg agcctcttac cttctccgac gtctctccgg ccgccctcgc
     1681 atttcagagg atcgccatgc gtctgacggg gaaagaggtc ccttttccga aactcgacga
     1741 ggaaaacggc aggggacttt tcgccggtat ccgcaagctc ttcgggatga agaactgaga
     1801 ggagaggagg ggtggcgatg gattttctca agaaattttt cagctcttcc gggtcgaaat
     1861 caagggcaaa agagcgtctc cagatcgtgc ttattcacga ccgggcggat atctctcccg
     1921 agatgctcga taatctccgg caggatatga ttgccgtgat cacgaggtat atggacatcg
     1981 atacatcgaa gatagagatg gacttcgacc gtgcggacag atccgtggcg ttcgtggcga
     2041 acatccctgt gatccgcatc aaacgagggg ttgcggcggc gagagaggga gaacggggct
     2101 agcgtgtccc cgcggaggag aacgccgtcg gaagcggaga cgccgcgtac gagggagaac
     2161 ttgtaatgga agaaaaaaaa ctttctcttg gcgagagtgt cgcaggcctg gacaaggcgc
     2221 agcttttttc cgtaggcgcg ctctttctgc tcggggtgct ctccatattc agcgccggcg
     2281 ccggttttga ggggcgggga gcggggtttg cgactcgcca gctcctctgg ggcgcagtgt
     2341 cgattgcggc ttttttgctc actctcaaga tagggtacga aaaatttctg cgggaggcgt
     2401 atctcctgta cggcctttcg ctgttgctct tgttgagcgt catggcgctg gggctcaccg
     2461 tgaaggggtc gcagcggtgg attgatctgg gtttcttcag gttgcaaccg acggagttcg
     2521 tcaagataac gctggttctg gtgcttgcca aacatctgtg caggtacccc ccgttgaact
     2581 ttctctcttt tctggggggg ctcgccgtgc ccgcgctttc ggggatcctg gtgctgattc
     2641 aaccggatgc cggaagcgcg atcgtctacg gcgtgatctc ctttgttgcg attgtcgtcg
     2701 ccggagcccc atggaggtat ccggcggggc tgttggccat cttcacgtcg cttcttccgt
     2761 tcgcatggcg ttttttgcgg gagtatcaga aaatgcggat acgcgtcttt cttgatccgt
     2821 ggatcgaccc gctcggcgcc gggtacaacg tcatccagtc ccggatagcc gtcggctcgg
     2881 gggggttgtt cggcgccggc tatatggagg ggctgcaaag caaactcaag tttttgcccg
     2941 agccgcacac cgacttcatc ttcggcgtct tctcggagga gttcggcttt atcggcgctc
     3001 tgggaatgct cgccctcttc ggccttctgt tctggcgcct cctggacgcc ggattgcgca
     3061 ccaaggatgg gagggccaag gtccttatcg ccgggatttc aacctggttc tggtttcaga
     3121 tgctgcaaag catcggcatg agcatggggc tcctgcccat aacgggcctt ccccttccgc
     3181 tcgtcagtta cgggggcagt tcgctgctct cgttttccgt tgcgctcgga cttgctcaga
     3241 gcgtgtacgt ttcgacgctg aaaagatact gagcatcgat tccgagccgg gaacaatcgg
     3301 ggcggaaccg tgaagaatcc tgctgtcggc aggtgtgaaa tcgagggagg aattgcaatc
     3361 tatgaagtgc agaaacgaat ccgcggcgcc ggagcagcgt gccgtcccgc gcgaagaagc
     3421 cgttcagccg gctctcttcg agtacggcga cgggatgtgg ccgctcctct ccaatgtgaa
     3481 gcgctcctcc cggtacgcgg ggtgcgagta cggcgtcata cctcccaagg acgggaattc
     3541 cattgtgcgt ttctgcctcg ctttccccga tgtgtacgag ataggaatga gctatctcgg
     3601 attccagatc ctctacttcc tcctgaagaa tctcccgtgc ggcgacgcgg accgggtcta
     3661 ctgcccgtgg accgacctcg aggagcagat gcgggcgcgg gagacgccgc tgacctccct
     3721 tgaaagcggc cgtcctctcc gcgagttcga cgtggtcggt ttcaccttgc agtatgaact
     3781 gagctacacc aacatcctca ccatgctcga ccttgggcgt atcccgctgc tttcggccga
     3841 caggggcgag aatgcccccc tcgtcgcggc gggaggcgtc ggcgcgctct ccccggaacc
     3901 gctcgctccg tttatcgact tcttctgcgt cggcgacgga gaggagctgc ttccggaggt
     3961 gatcacgctt ctttccggcg ccaaggggct ctcccggaga gaaaaacttg agttgctctc
     4021 cgggatagaa ggggtgtacg ttccctctct tgtcgaatgc gacgtcggcg agggaggcgt
     4081 cagtttccgt tctgcggcgg agaagcgcgt cgcgctcccc gttcggcggc gcatcgtcga
     4141 atcgctggac gacgcctttt tcccggagat gctcctcgtg ccgtcaggag gaattgtcca
     4201 cgaccgtgtt gccgtggaac tcttccgcgg ctgtaccagg ggctgccgtt tctgccaggc
     4261 gggcatggtg acccgtcccg tcagggagcg ctccgtccgt cccctcgcgg agcgaatcgt
     4321 ttcgctggtc gagaagacgg ggtgggagga ggttggtctc ctttccctcg catcctgcga
     4381 ctactccggc atcggcgaac tcgtcaggga gctctcgccg atgctggcgg aaaagaacgt
     4441 caaactgagc ctgccgagcc tccggatgga cgcattctcg gtgaatcttg ccgcggagat
     4501 ggagtcgatg cggcgcggag gtatcacctt cgctccggaa gcaggtacgc agcgtcttcg
     4561 cgacgtcatc aacaagggag tgagcgaaga ggatttcgag aactctctcc gcaccgcgtt
     4621 cacgaaaggg tgggacaggg tgaagctcta cttcatgatg gggctgccca cggagacgga
     4681 cgaggatctc gacggtatcg tacgcctgac cgaacgcgcg tactccatag gtaagggaat
     4741 aaagaagaga gtagaggtcg ccgtctcggt ggccgggttc gtcccgaaac cgcacactcc
     4801 gttccagtgg gaggcgcagc tttccgtgga cgaactgcgg cggagggggc ggtacgtcaa
     4861 gaaccggctc aacgaagtcg gccggggaaa gatttccctg aagtaccacg aacccgagca
     4921 gtccttcatg gagggggtct tcgcccgcgg cgacagacgg ctggcgcctg tgctccttgc
     4981 cgcatggagg aagggcgcgc gcttcgacgg atggaccgag acgttttctt tttcccggtg
     5041 gatggaggcc ttcgaaga
//
LOCUS       DAOPKU010000175         5045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_17993_length_5045_cov_5.152104, whole genome shotgun sequence.
ACCESSION   DAOPKU010000175 DAOPKU010000000
VERSION     DAOPKU010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17993_length_5045_cov_5.152104"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK849_06705"
     CDS             <1..201
                     /locus_tag="PK849_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HPE65854.1"
                     /translation="LSDEEITRLVRKTFDFRPAAIIRDLELRKPQYRRLAAYGHIGRI
                     DLDPLPAWERTDRAETLAKALK"
     gene            453..779
                     /locus_tag="PK849_06710"
     CDS             453..779
                     /locus_tag="PK849_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65855.1"
                     /translation="MRLFVAVNEAVNNALFHANRDLPEACVELVAVDEGASLRISVRS
                     EGDGFEHDLRADPGVFSDFLRESGRGVQIILHVVDEVSIEESGSIVTLRMGKDDSQRR
                     NSSIPS"
     gene            789..1850
                     /locus_tag="PK849_06715"
     CDS             789..1850
                     /locus_tag="PK849_06715"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPE65856.1"
                     /translation="MKNALQTSSPFPLALEVAGFRFNGMNLLDMFGESLVMWETATGH
                     IVYMNEAARVLYDYTLAETAALSIEEIFPSRDEVADRSFSVITTSHRKKSGIFFPVQA
                     TCRTLAQDTEEIMMMVVADISPENLARDDVALASDIQRGFLPGDLVGEPDVEVRSIYR
                     PMFLISGDMFGYHWDPSARVLNGYIYDLMGHGVPAALQTSALMVLFRQAFEDEDRIGS
                     SLTEKLQWVNDTAAERFLSDSFAAAICFRLDLRKKKLSYCAAGINAFLHGVNGELKKV
                     PTPGSLLGLSSRVEFGSGEVSVKPGDYFLFTSDGFLDLLEGTETLRMEFPDAYDHLFQ
                     IGYTGASRDDLSALCVHIR"
     gene            complement(1877..3133)
                     /locus_tag="PK849_06720"
     CDS             complement(1877..3133)
                     /locus_tag="PK849_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65857.1"
                     /translation="MNDHLSEETSAKIARLEEALAKSEDANKKKNAFIANLSHEIRTP
                     LNSIVGVVNLFQGTKLDLEQKKFVEMLSSSSRDLLLLVEELLDLSRLETGRLELKSDL
                     VPLRSCCSQAVRPLAMTAQERRLQMELSIAPDIPEFLCGDSARLRQVLRNIVGTAIAF
                     TAKGKIDIRVSIVHETRETITLQFSVAHSAEGIAGERINAMLDSSALDRSEFSRAYEG
                     VGLGLIIAKGLAEAMGGGLRAESVGNEHNIFHFTVAMKRTAENKQISEEPAAQPEQDI
                     PDALLALPGRLAILVVDDNKFNRSLTRTILRKKGGPEWNISLAEDGAEALKKISGEHF
                     DLVFMDIQMPGINGLDTTRAIRARERETLAQRLPVIAMTAYAMEGDRQMCLDAGMDDY
                     IAKPVDPDELVEVIIRNLRMITPDER"
     gene            complement(3260..4327)
                     /locus_tag="PK849_06725"
     CDS             complement(3260..4327)
                     /locus_tag="PK849_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPE65858.1"
                     /translation="MYKRNNRVFSMLSLLSLLLFFAVLPATEATGWQGVLHWSYDALS
                     SVSGSPAVAGDKILIGDDTGKLHALDRNTGRSLWIFDTKATINGAPAVFGENAYVGGT
                     DGTLFAVNLASGAVQWSYSPSEKYTPASIWASPAAGDGRVFFNSADGKTYALNATNGA
                     LAWTFDTGRELRSSPAYSDGLVFVGAYEGLFHALNAQTGKREWGGGSGGPIGSSATVS
                     NGVVYFGSWDGQLQAVRIKGVIPLWKYNAGESITTSPTVSGGKVFFGAINGKLVAVDT
                     ASGKLAWEFPVPGGIHSDPAVHEGLVFFGSDSGVFFALDAATGRERWRFESEREIMSS
                     PLVVDGIVYFGSYDGKVYAFR"
     gene            4470..>5045
                     /locus_tag="PK849_06730"
     CDS             4470..>5045
                     /locus_tag="PK849_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE65859.1"
                     /translation="MRVQLHAAAEKWLKEYIDSFRDENGSLHPLMEVKRTHSLRVRDN
                     ASQIASELGWSGEQLDCALTAALLHDTGRFPQFAEWGTYYDGASLDHGDLGEKTLMEH
                     FPDEKVATYGRWEDILLAVRLHNKKELPAGISPAAAPLVQIVRDSDKLDVFRLVRMHV
                     EEDRINELLPRIIPSGGYSEALVREVSSERRA"
BASE COUNT         1039 a         1379 c         1483 g         1144 t
ORIGIN      
        1 ctctcggatg aggaaattac gcgcctcgtg cgcaagacgt tcgacttccg cccggcggcg
       61 atcattcgtg atctcgaact tcgcaaaccg cagtaccgcc gccttgcggc ctacggacac
      121 atcggccgta tcgaccttga tccgctgccc gcatgggagc ggacggaccg ggccgaaact
      181 ctggccaaag cgctgaaata gcgccgattt aggttttctc cagagcggga ggctgcgata
      241 tgcggcctcc tttttctttt cctcgctctt tctctgtgct attatggccg taagaagacg
      301 aacggcgtcc cctatttccg gaggcgagga gtgatggtat gtgcagagag gagaacttga
      361 tatcgattgt attctccaca atagaggaat tcgcccctca aagaaacggt cttttaacgc
      421 ttatgtgctc aatgtgcggt tcgcagagag gcatgcgtct ttttgtagcg gtaaacgagg
      481 cggtcaacaa tgcgcttttt cacgcaaaca gggaccttcc tgaagcgtgc gtggagcttg
      541 ttgccgtcga tgagggcgcg agcttacgta tatccgtacg gagcgaaggc gacggttttg
      601 aacacgactt gcgtgctgat cccggcgtct tttccgattt tctcagggag tcgggccggg
      661 gagtgcaaat tattctccac gtcgttgacg aggtgagcat tgaagagtcg ggaagtatcg
      721 tcacgcttcg tatgggaaaa gatgattcgc agaggagaaa ctcctcgatt ccgtcatgag
      781 gtgatcgtat gaaaaacgct ctccagacat cttcgccctt ccccttggct ctggaagttg
      841 ccggttttcg ttttaacgga atgaatctgc tggatatgtt cggagaatct ctcgtgatgt
      901 gggagacggc gacggggcat atcgtgtaca tgaacgaggc ggcgagggtg ctctacgact
      961 acacgctggc ggagaccgcg gccctttcca tcgaggaaat ttttccttcc cgcgatgaag
     1021 ttgccgacag gtcgttctcc gtgattacga cctcgcacag aaagaagagc ggcatcttct
     1081 ttcccgtgca ggctacgtgc cgcacgttgg cgcaggacac cgaagaaatc atgatgatgg
     1141 ttgtcgccga catctcgccg gaaaatctgg cgcgtgacga cgttgcgctc gcctccgaca
     1201 ttcagcgggg atttcttccc ggagatcttg tgggagagcc cgatgtcgaa gtgcggagca
     1261 tctaccggcc gatgtttctc atcagcgggg atatgtttgg ctatcattgg gatccttccg
     1321 cacgggtttt gaacggctac atctacgacc tcatgggaca cggcgttccc gctgcgctcc
     1381 agacgtcggc cctgatggtc ctcttccggc aggcgttcga ggacgaggac agaatcggct
     1441 ccagcctgac ggagaagctg caatgggtga acgatacggc tgccgagcgc ttcctttcgg
     1501 attcgttcgc cgcggcgatc tgtttccgcc ttgacctgcg caagaagaaa ctctcctact
     1561 gcgcggcggg aatcaacgcg ttcctgcacg gagtgaacgg ggagttgaaa aaggtgccca
     1621 ctccggggtc gctcctcggg ctttccagcc gggtagagtt cggatcggga gaggtctctg
     1681 tgaaaccggg ggactacttt ttattcacaa gcgacggatt ccttgatctg ctggagggaa
     1741 cggaaacgct gcggatggaa ttcccggatg cctacgacca tctcttccaa attggctata
     1801 caggagccag cagggacgat ctttccgccc tctgcgtcca tatccgctaa gccggcgcac
     1861 agcaaggctc acaaggttat cgctcgtccg gagtaatcat gcggaggttg cggatgatga
     1921 cctccaccag ttcgtcaggg tcgaccggct tcgcgatata gtcgtccatc ccggcgtcaa
     1981 ggcacatctg tctgtctccc tccatggcgt aggcggtcat ggcgatgacc ggaagacgct
     2041 gcgctagagt ttccctttcg cgtgcccgta tggccctggt tgtatccagg ccgtttatcc
     2101 cgggcatctg gatgtccatg aagaccaggt cgaaatgctc gccggaaatt tttttcaacg
     2161 cttcggcgcc gtcttccgcc agcgaaatgt tccattccgg gccgcccttt tttcgcagga
     2221 ttgtccgggt gaggctgcgg ttgaacttat tatcgtcgac gacgaggatg gcgagcctcc
     2281 ccggcaaggc gaggagcgcg tcagggatat cctgttccgg ctgtgctgcc ggttcctcgc
     2341 tgatctgttt gttctcggca gtccttttca ttgctactgt gaaatggaaa atattatgct
     2401 cgttcccgac actctctgcc cggagcccgc cccccatggc ctcggccaat cccttggcga
     2461 tgatcaatcc aagacccacc ccttcgtagg cgcgggagaa ttcgctgcgg tccagggccg
     2521 aagagtcgag catcgcgttg attctctcgc cggcgattcc ctccgccgag tgggcaacag
     2581 aaaactggag agtaatcgtt tcgcgggttt cgtggacgat ggaaaccctg atgtcgattt
     2641 tgcctttcgc ggtaaacgcg attgcggtac cgacgatatt tcggagtacc tgccgcagtc
     2701 gggcgctgtc tccgcagaga aattcgggaa tatcgggcgc tatcgagagc tccatctgaa
     2761 gacgccgttc ctgtgccgtc attgccaggg gccgcaccgc ctgcgagcag caggagcgaa
     2821 gaggtacgag atccgatttc agttcgagcc gccctgtttc gaggcgcgaa agatcaagca
     2881 gctcctcgac gagcaggagt aaatccctgg aggatgacga gagcatttca acgaattttt
     2941 tttgttcgag gtcgagtttc gtcccttgaa acaggttgac aactccaacg attgaattga
     3001 ggggcgttcg tatctcatgg ctcagatttg cgatgaacgc gttttttttc ttgttcgcgt
     3061 cctctgattt tgcaagggct tcctcaaggc gagcgatctt cgccgacgtt tcctcggaga
     3121 gatggtcgtt catgggcgtt cctcctactc tgggagcgat cctcattctg tagtgattat
     3181 aaaccggaag agaaaaaagg acaaagggga gaagacgctc ccctttgtcc tgaaacgctt
     3241 cgtttcgagc gggttggcgt tatctgaaag cgtagacctt tccgtcgtag ctcccgaagt
     3301 acacaatgcc gtcaaccaca agcggagacg acatgatctc tcgctccgac tcgaaccgcc
     3361 agcgttcacg tcctgtggcg gcgtcgagcg cgaagaacac tccggaatcg gagccgaaga
     3421 aaacgagccc ttcatgcact gccgggtcgg agtgaatgcc gcccggtacc gggaattccc
     3481 acgcaagctt tccggacgcc gtgtccactg caacgagttt gccgttgatg gcgccgaaga
     3541 aaactttccc ccccgatact gtaggggatg ttgtgatcga ttcccctgcg ttgtatttcc
     3601 agagaggaat gactcccttg atccggactg cctgcagttg gccgtcccag ctgccgaagt
     3661 agacaacgcc gttcgacacc gtcgccgaag agcctatggg tccgccgctg ccgccgcccc
     3721 actccctctt tcctgtctga gcgttcagag catggaagag cccctcgtac gcgccgacga
     3781 agacaagacc gtcgctgtag gccggcgaag aacggagctc tcttcccgtg tcgaacgtcc
     3841 acgcgagcgc gccgttcgtc gcgttcaagg cgtacgtttt cccgtccgcg ctattgaaga
     3901 agactcttcc gtctcctgcg gcgggggagg cccatatcga agcgggggtg tacttctcgg
     3961 aggggctgta actccactgc accgctccgg atgcaagatt gacggcgaag agcgtgccgt
     4021 cggtcccgcc aacataggcg ttttctccaa acacagcggg cgcgccgttt atcgttgctt
     4081 tcgtgtcgaa gatccagaga gagcgtcctg tatttctatc gagagcgtgc agtttgccag
     4141 tatcgtcgcc gatgagaatc ttgtcccccg cgacggcggg ggagccggag acggaggaca
     4201 acgcgtcgta gctccagtga agaacgccct gccacccagt cgcttcagtc gccggaagaa
     4261 cggcaaagaa aagaaggagc gaaaggagag acagcatcga aaaaactctg ttgtttcttt
     4321 tgtacatgaa caacacctct tttcttgggg tagagtactt ctagctgata ttatacccta
     4381 cttcggtgtt gttgcatcgt taaaataccg acgttgaaaa gacagggaaa caggggtaca
     4441 attcggtgcg ttgaagagaa gggagcgtgg tgcgcgtgca acttcacgcc gcggcggaaa
     4501 agtggttgaa agagtatatc gattcattcc gggatgaaaa cgggagcctg caccccttga
     4561 tggaagtgaa acggacgcac agtctccgcg tgcgggacaa cgcgtcgcaa attgcctccg
     4621 agctcgggtg gtccggggag cagcttgatt gcgcgctcac ggcggcgtta ctgcacgata
     4681 caggtcgttt cccccagttc gccgagtggg gaacctatta cgacggcgca tctctggatc
     4741 atggcgactt gggggaaaag acgctcatgg agcatttccc ggatgagaag gtcgcgacgt
     4801 acgggcgatg ggaggatatc ctgctcgccg tgcggctcca taataaaaag gagcttcccg
     4861 caggaatttc acctgcggcg gcgcctttag tccagattgt ccgcgacagc gacaaactcg
     4921 acgtctttcg tcttgtccgg atgcatgtgg aggaggacag aatcaacgag ctgttgccca
     4981 gaattattcc ctcgggaggc tattcggagg cgctggtacg tgaagtatcc tctgaaagga
     5041 gagcc
//
LOCUS       DAOPKU010000176         5032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18062_length_5032_cov_3.848101, whole genome shotgun sequence.
ACCESSION   DAOPKU010000176 DAOPKU010000000
VERSION     DAOPKU010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18062_length_5032_cov_3.848101"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PK849_06735"
     CDS             <1..635
                     /locus_tag="PK849_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPE65860.1"
                     /translation="SVLKKYHPLWINLHFNHPRELTPEAARACALLADAGIPLGNQSV
                     LLKGVNDCPYIFRDLNQKLMKMRVRPYYIYQCDLSRGIEHFRTSIGKGLEIMEYLRGH
                     TSGLAVPTFVVDAPGGGGKIPVMPNYVISRSDRKTILRNYEGVITVYTEPDDNQSKCL
                     GKCRDLCERAKATFSGGIQSLFEGNAVSIEPKELLREKRRKACEDSSKQE"
     gene            complement(723..1172)
                     /locus_tag="PK849_06740"
     CDS             complement(723..1172)
                     /locus_tag="PK849_06740"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPE65861.1"
                     /translation="MEQQETFEKRMRPAWRSFLTWWILSVAVAALAVFLSVRYTAPWW
                     TVAVALIPVVVIMLKRCSTEFIVKHDEVTLEKGLFAKNSVEVGMPQIRTIEVRQTVFQ
                     RMLGIGDIMIASSGTDTYEIIVSGIEEPKELRDVIQGFQRRAASAAE"
     gene            complement(1484..2674)
                     /locus_tag="PK849_06745"
     CDS             complement(1484..2674)
                     /locus_tag="PK849_06745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE65862.1"
                     /translation="MSSKTFNLLRPESSGFKLSLLSTGHFFNDLYASFLPTFIPTIIS
                     RLGITMAQAGFFSTFLGIIHIIFQPIIGYLSDRSANPWLVIWGPIMTCLGATMIPLSP
                     SYGAALFFVGLWALGSAMFHPQGHGGVGHVVPREKLTVSLALFAVAGTAGVTVSPLFA
                     VALVNTVGFRLMPVVAIIPVLLLGLFTWRTMPSISHESGDAMTPQKGLLATLKSVFVV
                     IYPVWALSTVRDAASQGVRMFLPIRIASEGGEIGMVGTVLFLIMLSSTISMVLIGKIA
                     DRWGKKRVLLGVMALTPLSLLAGVYTENWVSIFCFLIGTSAINATLPVTAAIAQEMVP
                     NSRGMATSIVMGLSWGFGNMLMAPLGKIGDLFGVQTTLLVVAFLPLIALPLLYTKQFR
                     EMAE"
     gene            complement(2868..3323)
                     /locus_tag="PK849_06750"
     CDS             complement(2868..3323)
                     /locus_tag="PK849_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65863.1"
                     /translation="MSKMMRRVAGILSLCIASLWCGCSAHANAVVLLDTPEAAMDLTE
                     RIMEKVADDNIESGIFLMRPFFRLPKEEFNILLEQSKLQIPVIKDRFGAPLEHELIEQ
                     RFIGKSLMRIVHLQKYKYHAMRWIFVFYNPDGSWYINSFNFDDKIKELF"
     gene            complement(3383..4768)
                     /locus_tag="PK849_06755"
     CDS             complement(3383..4768)
                     /locus_tag="PK849_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPE65864.1"
                     /translation="MIRRTLLFVHLFFFIYGASFAAALPIDEFIEVCGAGSLEEVHEA
                     VASGADVNATDADGWTALMAAADNGNTEVVAFLVDRGADIHRKDRCGTTALMAAAGGS
                     DTRSVQLLVNAGGNVNERNSGGWTPLFFAARFSEEPDVIALLIASGAEVDVAEADGET
                     PLMIAAWYGENPRIVAALLKGGADVHRKDTKGWTPLSYAACTNKNPEVIAALLRGGAD
                     VDERDEDDGTTPLMNAAACNRNPAVLSTLLRAGADVNAKDTKGCTPLQFAARYAENPE
                     VARILLDAGADVAARDAQWGRTPLLWAAKTSKEPKIVEALLDAGADIHARDNYGRTAL
                     MCAAEECCSHPDVVRALLRGGADVNALTSDGRSALFFAAAFQHNDTIVHLLLKEGADV
                     NIRDTAGVTSLLFAAEYGTRPEILLALIHNGADASVCDADGKRAVDYAEGNSYLKDTE
                     VVQVLKKVSPQ"
BASE COUNT         1321 a         1448 c         1209 g         1054 t
ORIGIN      
        1 gctccgtact gaaaaaatat caccctctct ggattaactt gcatttcaac catccgaggg
       61 agctcacccc cgaggccgcc cgcgcctgcg cccttctcgc cgacgcaggt attcccctgg
      121 gcaaccagtc ggtgctcctc aagggagtga acgactgtcc gtacatcttc cgcgacctca
      181 accagaaact gatgaagatg cgggtgcgcc cctactacat ctaccagtgc gacctgtccc
      241 ggggaataga gcacttcaga acctccatcg gcaagggact ggagattatg gagtacctgc
      301 ggggccatac ctccgggctg gcagtcccca cgttcgttgt ggacgcgccg ggaggaggcg
      361 gcaaaattcc ggtcatgcct aactatgtga tctcgcgctc ggacagaaag acgatactgc
      421 gcaactacga gggagtcatt accgtatata cggagccgga cgataaccag agcaagtgcc
      481 tcgggaagtg ccgcgatctc tgtgagcgtg cgaaagctac cttttcagga ggcatccagt
      541 ctcttttcga aggcaacgcc gtctcgatcg agccgaaaga actgctcagg gagaaacgtc
      601 gaaaggcatg cgaagattcc tcaaaacaag aataaaaaag aaagaagagg ggagttcacc
      661 acgcccctct tctttgtact tatcttgcat gtttaaaaaa agcgtattct gcggcgaaaa
      721 gactattccg ctgcgctcgc ggctctcctc tgaaaaccct gaatgacatc gcgaagctct
      781 ttgggctcct ctatgccgga aacaataatt tcatacgtgt ccgttcctga ggaagcgatc
      841 atgatatcgc ctataccaag cattctctga aaaaccgtct gccggacttc aatggttcgg
      901 atctgcggca taccgacttc aacagaattc tttgcaaaca gccccttctc cagagttacc
      961 tcgtcatgct tcacgatgaa ctccgtcgaa cagcgcttca gcatgatgac aacaacagga
     1021 atcaatgcca ctgcgactgt ccaccacggc gctgtgtaac gcacactgag aaaaacagcg
     1081 agcgcggcca cagcaacact cagtatccac cacgtcaaaa aacttctcca tgctggacgc
     1141 attctcttct cgaacgtctc ttgctgctcc ataaataaac ccctcccgtc acaaatttta
     1201 tagcttcttc gaaatcaacc gataaacacg cgccaacggg gtaccgccat tcagagcatg
     1261 gatgcttatt atctcatacg tgaaggctcg ttttcccgaa acgactctgc gctccgaaaa
     1321 aaacatttcc gacgcgctgt aaggacgacg catcggaaac attttacacc accttggcat
     1381 tccatggaag aagaaagcag cgaaagtacg caaaaaaacg aaacgagcaa gaacaaactt
     1441 cagatgcgag cgtttctcct ccacttacct caacagaaaa atactattcc gccatttccc
     1501 ggaattgttt tgtatagagc aacggcaacg caatgagcgg aagaaaggcc accacgagaa
     1561 gcgtcgtctg cacgccgaag aggtcgccga tcttccccag cggcgccatc agcatgttgc
     1621 cgaatcccca tgagagcccc ataacaatgg aggtcgccat accgcgggag ttggggacca
     1681 tctcctgcgc tatggcggcc gtgacgggga gcgtggcatt gattgcggaa gttcctataa
     1741 gaaagcaaaa aatactcacc cagttctcgg tgtacacgcc tgcaaggaga gagagcgggg
     1801 tgagcgccat cacacccagc agcactctct tcttccccca cctgtcggcg atcttgccga
     1861 taaggaccat gcttatcgta ctcgagagca tgatgaggaa aaggacagtg cccaccatgc
     1921 cgatctcccc tccttcggag gcgatacgaa tgggcagaaa catacggacg ccctggctcg
     1981 ccgcgtcgcg gacagtagag agagcccata cagggtagat gacgacgaag acggacttca
     2041 gcgtcgccag cagccccttc tgcggcgtca tagcgtcgcc gctctcatgc gaaatcgacg
     2101 gcattgtcct ccaggtgaac aagccgagta gaaggacagg gattattgcc accacgggca
     2161 tcaggcgaaa acctactgtg tttaccaagg ccaccgcgaa gagcgggctg accgtaacac
     2221 ccgccgtccc ggcaactgcg aagagagcca aagagacagt aagtttttcc cggggaacga
     2281 cgtgccctac cccgccgtgc ccctgcgggt ggaacatagc gcttccaagc gcccagagtc
     2341 cgacgaaaaa gagcgccgcc ccgtaggacg gagaaagcgg gatcatcgtc gccccgaggc
     2401 acgtcatgat cggcccccag ataacgagcc aggggttcgc cgagcggtcg gagagatacc
     2461 ctatgatcgg ctgaaagata atatggataa ttccgaggaa agtgctgaaa aagcccgcct
     2521 gcgccatcgt aatccccagc ctggagatga tcgtcgggat gaacgtaggc aaaaaagacg
     2581 cgtagaggtc attgaaaaag tgccccgtcg agagcagcga aagcttgaaa cccgaactct
     2641 cggggcgcaa cagattaaaa gtttttgacg acataaaaca aactcactcc ttgtgattat
     2701 actttaagct aaatactatc atacaccatg cagaatgtaa tagggggagg aaggtgaaaa
     2761 tacataatgc aaaaacggcc tttgcggaag gccgttttta cagcaacgga aagacgatcg
     2821 aaagtggaat tttaatcagg ctttcaagac ctttcagcga aaaaacctca aaaaagttct
     2881 ttgatcttat cgtcaaagtt aaaggaattg atataccatg atccatcggg attatagaaa
     2941 acaaaaatcc agcgcatcgc gtggtattta tatttctgaa ggtggacgat ccgcatcaac
     3001 gattttccta tgaaacgttg ttcaatgagt tcgtgttcca atggagctcc gaaacggtct
     3061 ttgatcacag gaatttgcaa tttactctgt tccagaagta tgttgaactc ttccttggga
     3121 aggcggaaaa aaggccgcat cagaaaaata ccgctctcga tattatcatc ggcgaccttt
     3181 tccatgattc gctcggtcag atccatggcg gcctccggcg tgtcgagcag aacaaccgcg
     3241 ttcgcgtgcg cggaacagcc acaccaaaga cttgcgatac aaagggaaag aatacctgca
     3301 acccgtctca tcattttact catagttatc gcctcccgat attcattttt cagccagtgt
     3361 acgtttccat gatacgaccg tcttattgcg gtgaaacttt ttttaaaacc tgtacaacct
     3421 cggtgtcctt cagatagctg tttccctccg cgtagtcgac tgcacgcttt ccgtccgcat
     3481 cgcaaacgct cgcatcggct ccgttgtgga tcaacgcaag gagaatctcc ggcctggtac
     3541 cgtactcagc agcaaacaaa agagacgtta ctcccgccgt atcccgtata ttcacatccg
     3601 ccccctcctt caacagaagg tgaacgattg tatcattgtg ttggaaagcc gccgcgaaaa
     3661 aaagagcgct ccgcccgtcg gaagtcagcg cattcacatc ggcccctcca cgcaagagcg
     3721 cgcggacgac atcaggatgt gaacagcact cttcggccgc gcacatgagc gcagtgcgcc
     3781 cgtagttatc ccgcgcgtga atatccgctc cggcatccaa aagcgcctcg acaatcttcg
     3841 gctccttcga agttttagcc gcccatagaa gaggcgtccg cccccattgc gcgtcccgcg
     3901 cggctacgtc cgctcccgcg tccagcaata ttcgggcaac ttcgggattc tcggcgtacc
     3961 tcgccgcaaa ctgcaaagga gtacaccctt tcgtatcttt tgcattcaca tcggcgcccg
     4021 ccctcaggag cgtcgaaagt accgcaggat tccggttgca cgcagcggca ttcatcagag
     4081 gcgtcgttcc gtcgtcttca tcccgttcgt cgacatccgc ccctcctcgc aacagcgctg
     4141 caataacttc cggattttta tttgtgcacg cggcgtatga aagaggcgtc caccctttag
     4201 tatccttccg gtgcacgtcg gccccgccct tcagcaaagc cgccacaata cgcggattct
     4261 cgccgtacca tgccgcgatc ataagaggcg tctcaccgtc tgcttcagct acgtcgactt
     4321 ccgcgcccga tgcaatcaga agcgcgatca cgtcgggttc ctcgctgaaa cgggctgcaa
     4381 agaaaagcgg cgtccaccct cccgagttcc gttcattgac gtttccccct gcatttacaa
     4441 ggagttgtac cgaacgcgta tccgaaccgc cggcagccgc cataagcgct gttgttccgc
     4501 atctgtcttt tctatgaata tccgccccgc ggtcaaccaa aaatgcaacg acctcggtat
     4561 ttccgttgtc cgccgccgcc atcaaagcgg tccatccatc cgcatccgtt gcatttacat
     4621 cggcccctga agcgacggcc tcatgcactt cctcaagaga accggcgccg catacctcaa
     4681 taaattcatc gataggtaga gccgctgcaa aagaagcgcc gtagatgaaa aagaaaaggt
     4741 gcacgaacaa aagcgttctt cgaatcatcg aactcaccct tcctgatgaa gaggcaacat
     4801 ctatgaggag gctcgttaca atcatacacg aattacatta aaaatcacaa aattttcgcg
     4861 tggcatagag gattccgctt gatatggaga gatctagaac acaaaaacac aatttttatt
     4921 ttttaagaat gtacagatcg caaactcttc ggagatgcga ttttcaccgt tcacttcgat
     4981 agttatcccg ggcgggcaaa aacagccagc cagatgcagg gcggttccaa gc
//
LOCUS       DAOPKU010000177         5024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18107_length_5024_cov_3.952103, whole genome shotgun sequence.
ACCESSION   DAOPKU010000177 DAOPKU010000000
VERSION     DAOPKU010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18107_length_5024_cov_3.952103"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PK849_06760"
     CDS             complement(<1..730)
                     /locus_tag="PK849_06760"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HPE65865.1"
                     /translation="MVWEMERLSRSLHFISSGRKDQNPMPLLFFLIFIFIFPWFFLLL
                     VLIFFVLIPLGFSIQSFLWILTRPAQLFRIVTSRQVRKNHALAHGTLRILEEEYGRLN
                     AEGMPVEDGFSLRGGVDPETALAAAKTALDRIRRGDRSLAFFHRCGITVVMANTLFAF
                     LLFFVLRMLGLFSFLSAILCLVAVYFAGSRLSYLAQVYGTISPDLDDMEITGVDVGNE
                     QFALGGLSFLTPSSIFIRTRRAGEP"
     gene            complement(758..1453)
                     /locus_tag="PK849_06765"
     CDS             complement(758..1453)
                     /locus_tag="PK849_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPE65866.1"
                     /translation="MFPFFFDPTMILLIPALLLAMWAQMRVKSVFANYSRVGSRRGVT
                     ASAVARALLDRFGLRDVPVQRVGGNLTDHYDPRDKSLSLSDSVYGSSSIAAIGVAAHE
                     VGHAIQDSVNYSPLKIRNAIVPVVGIGSTLAFPLFFIGILLRGQMMMDLGIVLFLGVI
                     LFHLVTLPVEFDASNRALRVLADTGMLSTDEIGGAGAVLRAASWTYVAATVMAFAQLI
                     RLLFLRGMYGSRD"
     gene            complement(1567..2700)
                     /locus_tag="PK849_06770"
     CDS             complement(1567..2700)
                     /locus_tag="PK849_06770"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPE65867.1"
                     /translation="MATKIPTLDLVRNYSRIKDEILEAVHGVLETQHFILGPDVSLFE
                     KECEQYLGGVSAVACASGTDALILALMSLDIKEGDEVITTPYSFFATASCITRLGAVP
                     VFVDVDPATFNVDMNKVLEATTPRTKVFLPVHIFGQMAPLEDGMKELTSRGITVVEDA
                     AQAFGAWRSVEGDILRAGAVGRIGCFSFFPTKNLGGYGDGGMVVSTDTQLADRLRKLR
                     VHGAGTTYFHDEVGLNSRLDSLQAAILRVKLRHLEEWNEERRIVAGRYYALFAEKGLL
                     ETVQPPVELEGNHHIYHQYVVRVPQRDDLQRFLEEEGVVSRVYYPLSLHLQRCFSFLG
                     YKQGDFPVSERLSKETLALPVFPELSLEEQRRIVAAIGKFYGK"
     gene            complement(2866..3525)
                     /locus_tag="PK849_06775"
     CDS             complement(2866..3525)
                     /locus_tag="PK849_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE65868.1"
                     /translation="MREPRLYTTSADYAYQELRHRIITKQLKPGQRLPEVNIAVQMGV
                     SRTPVREALRRLASEGLVLIIPNSGARLAAPTIREIEDTFLVREQLECLAIALAAERI
                     GERHLRRIEEALVEEARAIEDKDLETYLEVNEAFHKTVADASGNRVLAEYVENILART
                     NAYVVFYDPFYHVETSPSPDDHRDILASLRAHDKEKSVALMKAHLEEAVAGLGKIVVE
                     E"
     gene            3711..3932
                     /locus_tag="PK849_06780"
     CDS             3711..3932
                     /locus_tag="PK849_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65869.1"
                     /translation="MLVLSMSAVVFFGYAVAGLAGFLLGRGRSDYIIFGLLLGFASTA
                     ASLLLWRHWLSLLEKEALHTADNTGEKLS"
BASE COUNT         1344 a         1333 c         1201 g         1146 t
ORIGIN      
        1 caggctcgcc ggctcgtctt gtccgaataa aaatacttga tggagtaaga aaggagagcc
       61 cccccagcgc aaattgctcg tttcctacat ccactcccgt aatctccata tcatccagat
      121 caggcgaaat cgtgccgtac acttgagcga gataactgag gcgagatccg gcaaagtaca
      181 ccgctacaag gcagagaata gctgaaagga aactgaacag accaagcatg cgcaacacaa
      241 aaaaaaggag gaaagcaaaa agcgtatttg ccatcacgac agttatcccg caacgatgaa
      301 aaaacgccag cgaccgatcg ccccttcgga tacgatccaa agcagtcttc gcagccgcga
      361 gtgctgtttc cggatcgact cctcctctga gagaaaatcc gtcttcgacg ggcatacctt
      421 cagcgttcaa tcgtccgtac tcctcttcaa gaatacggag cgtgccatga gcaagggcgt
      481 ggtttttacg aacctgacgg cttgtcacaa tacgaaaaag ctgtgcaggc cgtgtaagaa
      541 tccagagaaa cgactggatc gaaaaaccaa gaggaataag cacaaaaaag atgaggacaa
      601 gcagcagaaa aaaccaaggg aaaataaaaa taaaaatcaa gaaaaaaagc aaaggcatgg
      661 gattctggtc ctttctcccc gaggaaataa aatggagcga ccgggaaagc cgctccattt
      721 cccagaccat cgcctctcgg cgaagctgaa tgcgttttta gtctctgctt ccatacatac
      781 cacggagaaa gagaagccgt ataagctggg cgaatgccat tacggtcgcc gcaacatagg
      841 tccacgatgc tgcgcgcaaa accgctcctg ctcctcctat ttcatcggtt gacagcattc
      901 ccgtatcggc aagaacacgg agcgcgcgat tacttgcgtc gaactcaacc ggcaaggtaa
      961 ccagatggaa gagtatcacc ccaaggaaga gaacaatccc aagatccatc atcatctgcc
     1021 cccgaagaag aattcctatg aagaaaaggg gaaacgcaag cgtcgagcca atcccgacaa
     1081 ccggtacgat agcattccgg atcttcaaag gggagtaatt cacgctgtcc tggatcgcat
     1141 gtccgacttc gtgcgcagct accccaatag cagcgatgct tgaacttcca tataccgaat
     1201 ccgaaagact caggctctta tcccggggat cgtagtgatc cgtcaagttg ccgcctactc
     1261 tttgaacagg gacatctctc aaaccaaatc gatcaagcaa ggccctcgcg acagccgatg
     1321 cagtaactcc ccgacgggag ccgacgcggc tgtagtttgc aaagacgctc ttcactctca
     1381 tctgcgccca catagccagt aaaagcgctg gaatcagcaa tatcatcgtc ggatcaaaga
     1441 aaaaaggaaa cactgtttct cacctccgct atatgctctc attctacgag gaaagggtac
     1501 actgcgttcc cctttcctcc atcggtattt tacacggttg tatgagaaag agcaatattg
     1561 gtcagactat tttccgtaaa atttcccgat tgctgcaaca atacgccgct gctcctctag
     1621 agagagttca gggaagaccg ggagagcaag agtttccttg ctaagccgtt ctgaaaccgg
     1681 gaaatcgcct tgtttataac caaggaaaga gaaacacctt tgcagatgaa ggctcagcgg
     1741 ataatagacc ctgcttacta ctccttcttc ctcaaggaaa cgctgaaggt cgtctctttg
     1801 cggaacccgt acgacatact gatgataaat atgatgattg ccttcgagtt ccactggcgg
     1861 ttgcaccgtt tccagaagac ctttttccgc aaaaagagca taatatcttc cggcgacgat
     1921 acgccgctct tcgttccact cctcaaggtg gcggagtttt acacgcaaaa tggccgcttg
     1981 aagcgagtcg agacggctgt tcaagccaac ctcatcatgg aaataggtag ttccggcgcc
     2041 gtgaaccctc agcttgcgaa gacgatctgc cagttgcgta tccgtcgaga caaccatccc
     2101 gccgtcgccg tatcctccca ggtttttggt agggaagaat gaaaaacaac cgatgcgccc
     2161 gactgctccg gctcgaagaa tgtccccttc aacagaacgc caggccccaa aagcttgcgc
     2221 tgcgtcttcg acgactgtaa taccgcggga agtaagctct ttcatgccat cttcgagggg
     2281 cgccatctgg ccgaaaatat gcacgggaag aaaaactttt gttcgcggcg tcgtcgcctc
     2341 aagaactttg ttcatatcga cattgaatgt cgcaggatcg acgtcgacaa aaacggggac
     2401 agccccaaga cgtgtaatac agcttgccgt tgcaaagaag ctgtacggcg ttgtgattac
     2461 ctcatccccc tcttttatat cgagcgacat gagcgcaaga atcagcgcat ctgttccaga
     2521 cgcgcacgca actgctgaca cgcctccaag gtactgctcg cactcttttt caaagagaga
     2581 gacatccggt ccgagaatga aatgctgtgt ctcaagcact ccgtgcaccg cttcaagaat
     2641 ctcgtctttt attcttgaat aattacgaac aagatctagc gtgggtattt tcgtcgccat
     2701 gaaaaaagta cctcccgtaa aagtgagcga aacggggtct cgctctcgta agaactccat
     2761 tgaatatctt cacttcattc ggcgaatttt ggcattgagc aaaaaaacaa gcgaacgctc
     2821 cgtccggggc gatcgcctaa acaacttgag aatgaagaaa gtagtctact cttcgacgac
     2881 aatcttccca aggcccgcaa cagcctcttc caagtgggct ttcatcaaag cgacgctctt
     2941 ctctttgtcg tgagctctga gacttgccag gatgtctctg tggtcgtcag ggctcggcga
     3001 agtctccaca tgataaaagg ggtcgtaaaa cacgacgtag gcattcgtcc gtgcgaggat
     3061 attctcaacg tactccgcga ggaccctgtt cccgcttgca tccgccactg tcttatggaa
     3121 cgcttcgttg acctcaaggt aagtttcaag atctttatct tcgatcgctc tggcctcttc
     3181 gaccagggcc tcctctatgc ggcgaagatg acgttctcca atacgttcgg cggcaagcgc
     3241 aatggcaaga cattccaact gctcgcgcac aagaaatgta tcttctatct cccggatagt
     3301 aggagccgca agccgggcgc cgctgttcgg tatgataagg acaagccctt cgcttgcaag
     3361 acggcgaaga gcttctctta ccggggttct gctaaccccc atctggactg ctatatttac
     3421 ttcgggcaga cgctggcccg gttttagttg ttttgtgatg atacgatggc gaagttcctg
     3481 gtatgcataa tcagcagacg tggtgtacaa acgcggctct ctcatggaag gacctccctg
     3541 taattttcta aattgatcga gagtatttca atcattgtat aacagtctgt attatatatc
     3601 ttgaaccact ttatcacatt caatgcaaaa ccacaagatg cggctgcttt ttttttgatg
     3661 ctataattgc ctttttgaaa agaaaagagg ggacgaacgt gcgatttttg gtgttggtgc
     3721 tttccatgtc cgctgtagtg tttttcggat acgcggtggc tggtcttgcc ggctttcttc
     3781 ttggaagagg aagatctgac tatattatat tcggcctgct cctcggtttc gcatctaccg
     3841 cagcgtcgct tctactttgg aggcattggc tctcactcct tgagaaagag gcgctgcaca
     3901 ccgcagataa tacgggagaa aaactctcct gacggcatgc ccagtttcag agaatttttc
     3961 cccatgcttt tttcagacgt gcaacagctt cattcaacaa ttcatcagga acgccggtta
     4021 tagaaaaacg gacagcggct attttccttt ttcgtagaga aaaacggctc ccgggagcga
     4081 cgcgtacgcc atgtctttct gcaagcaatg cagcctcaga tccatctgtc ttccctgtag
     4141 ggaaccagag aaacactccg ccctcaggtt tccggagagg cactgtactg aaatgactct
     4201 ttaaaagact gtaaagcgtt tccatgcgga ccgaaagaat tgttctggct ctttcaaccg
     4261 cggccgccag ctctccatcc tcaagaaaac gtgcgactac cagctggacg agagaggaaa
     4321 cgtatccctg tctcaaagaa cttatccgca aatattcctc cttgagaaaa ggaggaagca
     4381 gcgtgtatcc cagccgcatt cccgggaaga gcgcctggct gaaggagcct acataaacga
     4441 cgccttcccc ttcccccatc gcctttaagg ccgggatgga tttttctcca tatcggagtt
     4501 ctccgtaact gtcgtcctca agaatgaaaa aacctttttt tctcctgaca gagagtatcg
     4561 cctgacgcag aggatgcgac aatgtccggc ccgtgggatt ctgaaagctc ggcacaagat
     4621 acagtacgtc ttttgaagag agtgcgtcaa ggcaagagag agtactctca tcaagaagcg
     4681 gaagtgtgcg caacggaatc ctttcatttt cagctattga gctgatttca ctgtaggaca
     4741 gctcttccgc ccatagcttg gagcacccgt gctcacgaaa agaacgaaga gcagtggaaa
     4801 gagcgtcctt cccgcctgcg gtgacaacca cttcctccgg atgcgcagga atgcctcgca
     4861 gaactgcatg gtgcagaaga aggcgtctca atgaaggcaa tccctcggtc ggactccctt
     4921 caagtgcgtc gcccagcaag gagagagaaa gcccgcggca gagttctgaa agctctctga
     4981 gagggagcag atctcgggtg ggacattccg tatcgaaacg gaga
//
LOCUS       DAOPKU010000178         5010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18168_length_5010_cov_5.691625, whole genome shotgun sequence.
ACCESSION   DAOPKU010000178 DAOPKU010000000
VERSION     DAOPKU010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18168_length_5010_cov_5.691625"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK849_06785"
     CDS             <1..314
                     /locus_tag="PK849_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tungsten ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE65870.1"
                     /translation="GQGMLETLMIAGERKGYAFTDRGTFIKYEDSRKEQADLAIIVEG
                     DASLKNQYSVMAVNPAKWENIRYDIALQYIDWLISPSTQKAIADFQLLGKQLFFPNAA
                     R"
     gene            complement(395..604)
                     /locus_tag="PK849_06790"
     CDS             complement(395..604)
                     /locus_tag="PK849_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPE65871.1"
                     /translation="MKISARNQIRGKVTAIKEGAVEAQVTLDIGGGQTMVSVITMDSV
                     ANLGLKVGSEAVAIVKADSVMLAVN"
     gene            complement(690..1397)
                     /locus_tag="PK849_06795"
     CDS             complement(690..1397)
                     /locus_tag="PK849_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65872.1"
                     /translation="MYPLNILIAEPDMMLRTLYRKTVSEVPGYVFAGYADNGDALHSI
                     VACTAVNVVLLDILLPRFGSLEGFRAMRAEFPRTDFIILSSEKTPDVVRGTICSGAFD
                     YLIKPFEWERFERALAAYSEYHRSLVDRKRPWRQEDIDRLPGFRKQLPENSQPTPKGF
                     QETLLNRLFNLLRESSSPLSASEAGRMLGISRSSARRYLEYLADEGEIAVQYEFTQVG
                     RPRKLYSVHFRAKEGSV"
     gene            1712..2464
                     /gene="modA"
                     /locus_tag="PK849_06800"
     CDS             1712..2464
                     /gene="modA"
                     /locus_tag="PK849_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE65873.1"
                     /translation="MSTHAIRVLLLVIFLFSSSLCSPLPGAASEEDVYVAVSPGIAQC
                     VEDMYASFAAAGGGKLVFVKEATGPLAQKMDAGAPFALLIAADPGWPKWLYERGKLKD
                     MRVCANGHLVLWAEKEEFCCEEKLASLRIASPDPETTSHGTLAADYLKGLGLWDEGAA
                     SGKIVITKNALHAIMAVQAGSADVALVPASLAIEMEGVYVQLPVPPLPTVGGLSTAHS
                     PESAAAFWKFLRSPEAAPLWEKWGFEAAEVAQ"
     gene            2461..3219
                     /locus_tag="PK849_06805"
     CDS             2461..3219
                     /locus_tag="PK849_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPE65874.1"
                     /translation="MSRPKTLDRIAAVVRERLERDSTGLLNEPLAIGSLDVGQAIGDP
                     APFTDFALQRGEERLVEARFLNARGQAFTSFPSAWKGTLGELLDLPLEEDCSRAFFIA
                     GVNAVGRYLGWGGKTVHCKDASPKLCGEKMGARLSDELGARGRVAIVGYQPAILKGLT
                     GALGPERVCVTDLNPENIGRCVCGVTVLDGEKDLQRAAAWCDAALVTGSTLSNGTIDA
                     LLDAFSGKRIVFFGTTIAVPAELLGFERLCFEAE"
     gene            complement(3281..3475)
                     /locus_tag="PK849_06810"
     CDS             complement(3281..3475)
                     /locus_tag="PK849_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65875.1"
                     /translation="MEENNKLFCGKCMVEMLPAPTGFSYLGHAFRTDVLRCPSCGQVF
                     IPESLAKGRMAEVETTLEDK"
     gene            complement(3462..>5010)
                     /locus_tag="PK849_06815"
     CDS             complement(3462..>5010)
                     /locus_tag="PK849_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPE65876.1"
                     /translation="DYLKTGAMRSAERVPPTRMRQPPEAIVRTPVVLPADGALFSKEE
                     AVAEASRCIRCRCDACVRHCGLMRSYKKFPGRIAEEVEITITPGTLDGNGTVATRLIA
                     SCNQCGLCAEICPERIDMGEFLRASHQAMREKGAMPQAFHDFWLRDMEFANSERASLL
                     APPPGGAICTRIFFPGCQLGASDPRYVKESYRFLLGLEPGVALRLGCCGAPAVWAGDM
                     ELFRQVRERILDDWRALGSPQMVMACPTCLEMFARYIPEIDCAPLVDVLAASGHPPAE
                     QGNGEIVSVFDPCASRNRPESQRAARSLVRGAGYELAPLPYERKRAQCCSYGGQIDIA
                     APKYARRLAEQRSKDGEAPYVVYCSNCRDVFAHAGKPARHILDIFFGLNGWNAAPPTF
                     DERRKRREAMKNDLVQEFWPDMELRGKEEIMDNRKSIAITPELREKMNRERLLEEDAL
                     AVIEECERSGRRVWDPSAERFFGCKEIGSMTQWVEYAPSPEGYALFNTYAHRMKIERE
                     DTPHGRK"
BASE COUNT          910 a         1494 c         1576 g         1030 t
ORIGIN      
        1 ccgggcaggg aatgctcgaa accttgatga tcgccggcga gcgtaagggg tacgccttca
       61 ccgaccgcgg caccttcatc aagtacgagg actcgcgcaa ggagcaggcc gaccttgcaa
      121 tcatcgtcga gggcgacgcc tccttgaaga accagtacag cgtcatggcg gtgaaccccg
      181 cgaagtggga gaatatccgc tacgacatcg cgctgcagta catcgactgg ctgatctccc
      241 cgtcgacgca gaaggcgatc gccgacttcc agctcctcgg gaagcagctc ttcttcccca
      301 acgccgcccg ctgaacgacg gcctcagtac gttttacaaa ataaaaaaag cccccgacag
      361 ggggcttttt tggtgtctga ttcttgcgga attactaatt gaccgcgagc atgacgctgt
      421 cggccttgac gatggcgaca gcttcggatc ccaccttgag gccgaggttg gccacggagt
      481 ccatcgtgat cacggagacc atcgtctggc cgccgccgat gtcaagcgtt acctgcgcct
      541 ccacggcgcc ttctttgatg gcggtcacct tgccgcggat ctgattgcgt gcgcttattt
      601 tcatgatatg cactcccttc tgttctagat tcttccggta ttatcgcacc taaatacatc
      661 ttttgcaagt ttgtgcagct gaaaagagtt cagacagatc cttcttttgc ccgaaagtga
      721 accgagtaga gctttctcgg gcggcctacc tgtgtgaact cgtactgtac cgcgatctcc
      781 ccctcatcgg cgaggtactc aaggtagcgc cgggcgctcg acctggagat gccgagcatt
      841 cgccccgctt ccgaagcgga aagggggctg gacgactccc gcaaaagatt gaagagtcga
      901 ttcagaaggg tttcctgaaa acctttgggc gtcggttggg aattttcggg aagctgcttc
      961 cggaaaccgg gaagcctgtc gatatcctct tgtctccaag ggcgttttct gtcgacaagg
     1021 ctgcggtggt actcgctgta tgcggcaagc gctcgttcaa atcgctccca ctcgaacggt
     1081 ttgatcagat agtcgaacgc gcccgagcag atcgttcccc tgacgacgtc gggcgttttc
     1141 tccgaggaga ggatgatgaa gtccgtcctt ggaaattccg cgcgcatcgc acggaatccc
     1201 tccaggctgc cgaagcgggg cagaagaatg tccaagagaa ccacgttgac ggcggtgcag
     1261 gcgacgatgg aatgcaaagc gtcgccgttg tccgcgtagc cggcgaagac atagccggga
     1321 acttccgaga cggtctttct gtacagcgtg cgaagcatca tgtccggctc ggcgatgaga
     1381 atgttcagcg gatacacttc cagctctccc ttctacggaa tgtcctggag attccctctc
     1441 ccaaggcgcg ctccattttt ttctgatagt gaacggcagg cccgcggcgg gagggaaaaa
     1501 actacattac cattatacat gaagcggacc gcttcttcgt ttttccggtt ttccgtgcgg
     1561 agagaaagag ggggcggcgc tgtgaactcc gaagtgaagg atgatttcta caagaggaat
     1621 cgagattgtg caacgatgtt gcatttctct gtttgaggtg gtattcttgc agaaaatgca
     1681 acgaagttgc aaaatccagg aggtgccttc ggtgtccaca catgcaattc gcgtgctttt
     1741 gctcgtcatt tttctttttt catcatcatt atgctccccg ttgcccggcg cagcgtccga
     1801 agaggacgtc tacgttgcgg tgtcgcccgg catagcgcag tgcgtcgagg acatgtacgc
     1861 ctcgttcgcc gcagcgggcg ggggaaagct cgtcttcgtg aaggaagcca ccgggccgct
     1921 ggcgcagaag atggacgcgg gcgctccgtt cgctctttta atcgccgccg atccggggtg
     1981 gccgaagtgg ctctacgaaa gaggcaagtt gaaggatatg cgggtctgcg cgaacggcca
     2041 tcttgttctc tgggcggaaa aagaagagtt ttgctgcgaa gaaaagctcg cctcgctccg
     2101 tatcgcctct ccggaccctg agacgacgtc ccacggcacg ctcgccgcgg actatctgaa
     2161 ggggctcggt ctgtgggatg agggagcggc gtcgggaaag atcgttatca cgaagaacgc
     2221 gctccatgcg atcatggccg ttcaggcggg gagtgcggac gtcgcgctcg ttccggccag
     2281 cctcgcgatc gaaatggaag gggtgtatgt acaacttccc gtccctccgc ttccgaccgt
     2341 cggcggcctg agcacggcgc attcgccgga gtctgccgca gctttctgga aatttctgcg
     2401 ctcccctgaa gcggctcccc tgtgggagaa gtggggcttt gaagccgcgg aggtcgcgca
     2461 atgagccggc cgaagacgct ggaccgtatt gcggcggtcg tcagggaacg tcttgagcga
     2521 gattccacgg gactcctgaa cgaaccgctg gcgataggct ctcttgatgt cggacaggcg
     2581 ataggagacc cggcgccgtt tacggatttc gccctccagc ggggcgagga gcgccttgtg
     2641 gaggcgcgct tcctgaatgc gagagggcag gcgttcactt cgtttccctc ggcgtggaag
     2701 ggaacgcttg gcgaactgct cgacctgcct cttgaagagg attgcagccg ggcctttttc
     2761 atcgccggag tgaacgctgt cggcaggtat ctcgggtggg gcggaaagac cgtgcactgt
     2821 aaggacgcct cgccgaagct gtgcggcgag aagatgggcg cgaggctttc ggacgaactt
     2881 ggcgcgcgcg gccgcgtcgc catcgtcggc taccagcctg cgattctgaa ggggctgacg
     2941 ggggcgctcg gcccggagcg cgtgtgcgtg acggatctga accccgagaa catcggacgc
     3001 tgcgtatgcg gcgtgacggt tctcgacggc gagaaggact tgcagcgcgc ggccgcgtgg
     3061 tgcgacgccg ccctcgtgac gggctccacg ctctcgaacg ggacgatcga cgcgctcctt
     3121 gacgcgttct ccgggaagcg tatcgtcttt ttcggaacga cgatcgcagt ccctgccgag
     3181 cttctgggct tcgagaggct ctgtttcgag gcggagtaaa gcgtgttccg gaggtgaaga
     3241 ggcggcgccg ctgtggttgt gccgtctctt cacctccgtt ttatttgtct tcgagcgtcg
     3301 tctccacttc ggccatgcgc cccttggcga gactttccgg gatgaagact tgcccgcacg
     3361 acgggcagcg cagcacgtcc gtgcggaagg cgtgtccaag gtagctgaac ccggtcgggg
     3421 cggggagcat ctccaccatg cacttcccgc agaagagttt attattttct tccatgaggg
     3481 gtgtcctccc gctcgatctt cattctgtgc gcataggtgt tgaagagcgc gtacccttcg
     3541 ggggacggcg cgtactccac ccactgcgtc atggagccga tctccttgca gccgaagaag
     3601 cgttccgcgg acggatccca tactctgcgg ccgcttctct cgcactcctc gatcacggcg
     3661 agcgcgtcct cctccagaag ccgctcgcgg ttcatcttct cccgcagttc gggagttatg
     3721 gcgatacttt ttcgattgtc catgatctcc tcttttcccc ggagttccat atcgggccag
     3781 aactcctgaa caagatcatt tttcatggct tcgcgccgtt ttctgcgctc gtcgaacgtc
     3841 ggcggcgcgg cgttccatcc gttcagcccg aagaagatgt cgagaatatg gcgcgcgggc
     3901 ttccccgcgt gcgcgaagac gtcgcggcag ttggagcagt agacgacgta cggcgcttcg
     3961 ccgtccttgc ttctctgctc cgccagccgg cgcgcgtact tcggcgcggc gatgtcgatc
     4021 tgcccgccgt aggagcagca ctgcgcccgc tttctctcgt aggggagggg agcgagttcg
     4081 tatcccgcgc cgcggacgag cgagcgcgcc gcgcgctgcg attccggtct gttgcggctc
     4141 gcgcacggat cgaagacgga gacgatctcc ccgttcccct gctccgcagg aggatgtccg
     4201 gatgcggcga ggacgtcgac gagcggcgcg cagtcgatct ccggaatgta gcgggcgaac
     4261 atctccaggc atgtcgggca ggccattacc atctgcggac tgccgagcgc gcgccagtcg
     4321 tccagaatac gctcccgcac ctgccggaaa agctccatgt cgcccgccca gacggcgggg
     4381 gctccgcagc atccaagccg gagggcgacg ccgggctcga gaccgagcag aaaacggtag
     4441 gactccttca catagcgcgg atccgatgcg ccgagctggc atcccggaaa aaagatcctc
     4501 gtacagatcg ctccgccggg gggtggcgcg agcagggagg cccgttcgct gttcgcgaac
     4561 tccatgtcgc gcagccagaa atcatggaag gcctgcggca tggcgccctt ctcgcgcatc
     4621 gcctgatggc tcgcgcgcag aaattctccc atgtcgatgc gctccgggca gatttcggcg
     4681 cagagtccgc actggttgca ggatgcgatg aggcgcgtgg ccaccgtgcc gttgccgtcg
     4741 agcgtccccg gggtgatcgt gatttcgacc tcctcagcga tgcgtcccgg aaacttcttg
     4801 tacgagcgca tcaggccgca gtgacggacg caggcgtcgc accggcagcg aatacagcgg
     4861 gaggcctccg cgacagcctc ttctttcgaa aagagcgcgc cgtcggcggg aagcacgacg
     4921 ggcgtgcgga cgatcgcttc cggcggctgc cgcatcctcg tcggggggac tctttccgcg
     4981 ctccgcatcg cgcccgtctt cagatagtcc
//
LOCUS       DAOPKU010000179         4982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18330_length_4982_cov_5.342196, whole genome shotgun sequence.
ACCESSION   DAOPKU010000179 DAOPKU010000000
VERSION     DAOPKU010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18330_length_4982_cov_5.342196"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>832)
                     /locus_tag="PK849_06820"
     CDS             complement(<1..>832)
                     /locus_tag="PK849_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPE65877.1"
                     /translation="FILVILAALLSGECAALPGSASPQLLENGEEPVSEYRDERIGDV
                     LSADDAGQKRILPSKGPYIALVIDDFGFSRKMTAEFESLRLPVTWAILPFERFSGYTA
                     ERAKETGIPYIIHLPMGAKSDTARAEGMEGNVVDTGMSPEEIADVLLNAVEVLPDAVG
                     VNNHRGSRATADEELMDSFMEILSGFSLFFLDSRTSASSAAYAKAVEKGVPALYSSVF
                     LDHQADETFMETQFLRALALAKRRGWVVVIAHTRIATLQYLKKLEGALPDGYLFVSLP
                     D"
     assembly_gap    833..842
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(904..2160)
                     /locus_tag="PK849_06825"
     CDS             complement(904..2160)
                     /locus_tag="PK849_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPE65878.1"
                     /translation="MHKELSPLWRRVRGWIAGMVVGVVVGVAVTGGLLAAGAMDLGEF
                     ARISPFNARAMWLMKQARAIVETYQVDSGTKTVEEDDLLYGAIHGMVAAWGDPYTRFV
                     DPSQLKEEEIEMEGQYGGLGIYIGQRDGRTLVISPIEDTPADRAGLKPQDQIVKIEDE
                     IILGWDSQKVVKHLRGAPGTSVTIWVRREGEEELLKFELAREIIKLKSVRSEMMEETL
                     GYIKLSQFKQNTADEFESALRELIGDGAKGLILDLRNNGGGLLNGAMEICDMFLDGGL
                     VVGMKGRVERANDEMFASSGTLTDLPMVVLINEGSASASEIVAGALADRGRALSVGKK
                     SFGKGSVQTLFNLGDGSGIYVTIARYQTPSGKVIDNIGLEPDIDVEGEVTKEAEKDEQ
                     LKRAKLELRDLISGKRATVPKVPSDF"
     gene            complement(2185..3384)
                     /locus_tag="PK849_06830"
     CDS             complement(2185..3384)
                     /locus_tag="PK849_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPE65879.1"
                     /translation="MKKTTVLSVLLLAAFISLFYSAPYCEAASDIDSKLVQEEARMRT
                     LERQISEHQQRVRQMGEREQGVISRIEELDQKRKMTEQRIRVLELRSAKTKQAVEDLR
                     REIQATEQSLVSMKRVLEDRLVNIYKYGGVAELNLLLSASTAHEAMETTLLLNRIALQ
                     DETMITDMLVKKERLGEAAAQLEAERNQLAINSAALEKSRKTYRQEIASSDSFLQKVR
                     SERALHQRAVKELQDSQREIQSTIMDLMRKKREAEMRPDAPPVTREEVPSGGKLAWPL
                     PSKGRITSSFGMRVHPTFKTRMMHTGIDIQMPSGTPVYAAGPGEVLYAGWLRGYGQIV
                     IIDHGRDLSSVYAHLSRLAVGEGDRVKKGQNIGAVGTTGTTTGAHLHFEVRVNGEAKD
                     PMKYLGK"
     gene            complement(3411..4295)
                     /locus_tag="PK849_06835"
     CDS             complement(3411..4295)
                     /locus_tag="PK849_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPE65880.1"
                     /translation="MASFRYAFRDTFRLLFRHWGLSLLTLLTAASVLYILGFASLFAM
                     NMRYMISRIEGALVVQAYMKRGETAEQALELVRSSPLVLSVRAISPEEALERLRAKLG
                     NQAQAVTLLGENPLPWSLEIQVQRAEHITPLVRDLMVLPSIEEVLYAGKLVERLATIS
                     RASSAVSGIILMLTLLISTLVIFNTIRIAIYSRKEEIEVMLLIGATSTYISLPFVIQG
                     MLLGAGGAILAIFGLWSTYSSAIEAIRSTLAFVDIIDNRRILAQFYVFLFAAGATTGW
                     MCSWLAVHRFTSQSMRSR"
     gene            complement(4282..4977)
                     /locus_tag="PK849_06840"
     CDS             complement(4282..4977)
                     /locus_tag="PK849_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE65881.1"
                     /translation="MDIRLSGITKVFEPDIVALEDIYLSVDSGEFLYLVGTTGSGKTT
                     LLRLISREYLPTRGQITVGDRNLRKLRSSELPYYRREVGVVFQDFKLLPNLTVFENVA
                     FVLEAMGIPPKLVESRSNEVIDQVGLWRRRFLKPPQLSGGEQQRVAIARAMANSPAVF
                     LADEPTGNLDLRTSEEIMRLLLSINASGTTVIVATHDQYLVDAYRQRLVELHNGRLKR
                     DERRGRYFVDGEL"
BASE COUNT         1035 a         1565 c         1304 g         1068 t
ORIGIN      
        1 aatccggaag ggagacgaat aggtagccgt ccggaagcgc tccttccaac tttttcagat
       61 attgcagcgt cgcgatcctc gtgtgcgcaa tgacgacaac ccacccgcgc cgtttcgcaa
      121 gagcgagcgc acggagaaac tgcgtttcca tgaacgtctc gtccgcctga tgatccagga
      181 agacagaaga atagagagcc ggcacgccct tttcgacggc ctttgcatag gccgcggaag
      241 atgcgcttgt ccggctgtcg agaaaaaaca aagaaaaacc gctcaatatc tccataaaag
      301 agtccatgag ctcttcgtcc gcagtcgccc tcgatcccct gtggttgttg acccccacgg
      361 cgtccggcag tacttcaacg gcattgagca ggacatccgc aatctcctcc ggcgacatcc
      421 ccgtatcaac gacgttgcct tccataccct cagcccgggc cgtatcgctc ttcgctccca
      481 tgggcagatg aatgatgtag ggaatccctg tctctttcgc tctctccgcc gtatatccgg
      541 aaaaacgctc gaaaggcaga atagcccacg ttacagggag tctcagcgac tcgaattccg
      601 cagtcatctt ccgtgaaaag ccgaaatcat caatcacaag agcgatatac ggccccttgg
      661 aagggagtat gcgtttctgt ccggcgtcat ccgcagacag gacgtcgccg atgcgctcgt
      721 cccgatattc cgaaacaggt tcttcgccgt tctcaagtag ctgcggggag gcgcttccag
      781 gcagcgcagc acactctcca gagagcagcg ccgccaagat cacgagaatg aannnnnnnn
      841 nnctcctcaa ggccgttgca taccgtcctc tcttcatcaa ccgacatcag gcgccaagat
      901 tgctcaaaaa tcagatggta ctttcggcac ggtcgccctc tttccggaaa tcaagtcccg
      961 cagctccagc ttcgcccgtt tgagctgttc atctttttcc gcttccttcg ttacttcccc
     1021 ctccacatca atatccggtt ccaggccgat attgtcgatc acctttcccg aaggggtctg
     1081 ataccgtgcg atggtgacat atattccgga gccgtctccc agattgaaga gcgtctgcac
     1141 cgagcccttc ccgaagcttt tctttcctac ggacaacgcg cgccccctgt ccgcgagagc
     1201 tcccgcgact atttcggagg cggacgcgct cccctcgttg atcaggacga ccatcggaag
     1261 atcggtcagc gtcccgctgc tggcgaacat ctcatcgttc gccctctcga cgcgcccctt
     1321 catcccgacg acgaggcctc cgtcgaggaa catgtcgcag atctccatcg cgccgttcaa
     1381 caaaccgccc ccgttgttcc ggaggtcgag aatcaaccct ttcgcgccat cgccaatgag
     1441 ctcgcgaagc gcgctttcaa actcgtccgc ggtattctgc ttaaactgtg agagcttgat
     1501 ataccccagc gtttcctcca tcatctccga ccggacggac ttcagcttga ttatttcgcg
     1561 ggcgagctcg aacttcagga gctcctcctc cccctcccta cgaacccaaa tggtgacgga
     1621 cgttcccgga gcaccgcgca gatgcttcac gaccttctgc gaatcccatc cgaggatgat
     1681 ctcatcctcg attttcacga tctgatcctg gggtttgagg ccggcccggt ccgccggagt
     1741 atcttcaatc ggactgatga cgagcgtcct gccgtcgcgc tgtccgatat agatgccaag
     1801 accgccgtac tgcccctcca tctcgatctc ttcttcctta agctgagagg ggtcgacaaa
     1861 cctcgtatac gggtcgcccc acgccgcgac cattccgtga atggctccgt acagcaggtc
     1921 gtcctcttcg accgtcttcg ttcctgaatc tacctggtag gtctccacta ttgcacgtgc
     1981 ctgtttcatc agccacatcg cgcgtgcgtt gaacggtgat attctggcga actcgcccag
     2041 atccatcgct cctgcggcga gcaagcctcc cgtcacggcg actccaacga caacgccgac
     2101 gaccattccg gcgatccagc ctcgtacccg cctccagagc ggcgatagtt ccttatgcat
     2161 tcttcttctc ctttgtttta cggtttattt cccgagatac ttcatcggat ccttggcttc
     2221 gccgttgact ctgacctcga aatgaagatg cgctcccgtc gtcgtcccgg tagtgccgac
     2281 tgcgccgata ttctgccctt ttttcacacg gtccccttcg ccgaccgcaa ggcgcgaaag
     2341 gtgcgcgtac acgctggaga gatccctgcc gtgatcgatg atgacgatct ggccgtagcc
     2401 gcgaagccat cccgcgtaga ggacctcgcc gggacccgcc gcgtagaccg gagttccgct
     2461 tggcatttgt atatcgattc cggtgtgcat cattcgcgtc ttgaaggtcg ggtgcactcg
     2521 cataccaaaa gaactggtga tccgtccctt cgaggggagc ggccaggcaa gtttccctcc
     2581 tgacgggacc tcttcgcgag taaccggagg cgcgtcgggg cgcatctccg cctcgcgctt
     2641 cttccgcatc agatccatga tggtggactg aatctcccgc tgagagtcct gaagctcctt
     2701 gacggcccgc tgatggaggg cgcgctcgct ccggactttt tgaaggaagc tgtcgctgct
     2761 cgcaatctcc tgccggtaag tcttcctgct cttttcgaga gcagcggagt tgatcgcgag
     2821 ctgattccgc tccgcctcaa gctgcgcggc cgcctcgccg agacgctctt ttttcacgag
     2881 catatcggtg atcatcgttt cgtcctgaag agcgatgcgg ttcaagagca gcgtcgtctc
     2941 cattgcttcg tgggccgtgg aagccgagag cagcagattg agctcggcaa cgccgccgta
     3001 tttatagata ttcaccaggc gatcttcaag cacgcgcttc atcgagacca gagactgttc
     3061 ggtcgcctga atctcgcggc gcagatcctc gaccgcctgt tttgttttcg cgctcctcag
     3121 ttcgagcacg cgaatgcgct gttccgtcat cttccgcttc tgatcaagct cctctatccg
     3181 ggagataacg ccctgctccc gctcccccat ctggcggaca cgctgctgat gttcggatat
     3241 ctgccgttcg agcgtccgca tccgcgcctc ctcctgaacg agtttcgagt cgatatcgga
     3301 ggcagcctcg caatacggcg ccgaatagaa cagggaaatg aacgctgcga gcagaagtac
     3361 ggagagaacc gtcgtttttt tcataccgcc aacctccttc acggacagga ctaccgggaa
     3421 cgcatactct gagatgtgaa acgatgcacg gcaagccagc tgcacatcca gcccgtagtg
     3481 gctccggcgg cgaaaaggaa aacgtaaaac tgggccagaa tgcgccggtt atcaattata
     3541 tcaacaaacg cgagcgtcga acggatagct tcaatggcgg aagaataggt gctccacaaa
     3601 ccgaagatag ccaatatcgc accgcccgcg ccgagaagca tcccctggat gacgaacgga
     3661 agcgagatat aggtcgacgt cgctcctatg agaagcatca cttcgatctc ttctttccgg
     3721 gagtagatgg cgatgcgaat tgtattgaaa ataacgagcg tacttatcag cagggtcagc
     3781 atgagaatga ttcccgatac cgcgcttgat gcccgggaga tcgtggcgag acgctccaca
     3841 agtttcccgg cgtagagcac ctcctcgatc gacggaagca ccataaggtc gcggacaagc
     3901 ggcgtgatgt gctccgccct ctgcacctgg atttccagac tccacggaag aggattctct
     3961 ccaagcagcg tgaccgcctg cgcctgattg cccagctttg cacgcagtct ttcgagcgcc
     4021 tcctccgggg agatggcgcg aacggacaaa accagagggg acgaacggac aagctcgagc
     4081 gcctgctccg ccgtctctcc tcgcttcatg tacgcctgga cgacgagggc gccctcgatc
     4141 ctggagatca tgtaccgcat gttcatagcg aagagcgacg cgaatccgag aatatagagg
     4201 acggaagcgg ctgtgagcag cgtgagaagg ctcagacccc agtggcgaaa gagaagacgg
     4261 aatgtgtccc tgaaagcgta tctaaagctc gccatcgacg aaatacctcc ccctgcgctc
     4321 gtcccgtttc agccgtccgt tgtgcagctc cacgaggcgc tgcctatagg cgtccacgag
     4381 atactgatcg tgcgtcgcga caatcaccgt ggtccccgag gcgttgatgg agagcaggag
     4441 acgcatgatc tcctccgagg ttcgcagatc aaggttgccg gtaggctcgt cggcaaggaa
     4501 tacagccggc gagttcgcca tcgcccgcgc gatcgccacc cgctgctgtt ctccgcccga
     4561 gagctggggc ggctttaaaa aacgcctcct ccagagtccc acctgatcga tcacttcgtt
     4621 ggacctgctc tcgaccagct tcggcggaat acccatcgct tccagtacga acgccacatt
     4681 ctcgaacacg gtcaagttcg ggaggagttt gaaatcctga aagactacgc cgacctctct
     4741 ccgatagtac gggagctcgg aagagcgaag cttcctcaga tttctgtctc cgacggtgat
     4801 ctggcccctg gtggggagat attccctgga gatcagcctg agaagcgttg tctttcccga
     4861 tccggtggtc cccacaaggt agagaaattc accgctgtcc accgagagat agatatcctc
     4921 aagcgcgacg atatcgggtt cgaacacctt cgtgattcca gacaggcgaa tatccatcca
     4981 tt
//
LOCUS       DAOPKU010000180         4951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18465_length_4951_cov_4.113971, whole genome shotgun sequence.
ACCESSION   DAOPKU010000180 DAOPKU010000000
VERSION     DAOPKU010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18465_length_4951_cov_4.113971"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK849_06845"
     CDS             <1..285
                     /locus_tag="PK849_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65882.1"
                     /translation="VGRLKRRQIPGILDRNITARRDLSSERYFCKSGLFCSIARLSTN
                     HNETPFTNKEILKNNVQTVLLTCRPEDYIIFPSVSQGSSTQNLIKGVCKK"
     gene            282..1265
                     /locus_tag="PK849_06850"
     CDS             282..1265
                     /locus_tag="PK849_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE65883.1"
                     /translation="MKRRVLLLLALALVVSFSGTAFAAKEGWPDQLRFMAGPPGGNWF
                     ALGGALADMWSKEVLQTTSITGGGVANVVNTDNSKGDLGFSVTSFVGAAGKGEDPFKA
                     PAKNAVVMSNIYTQFTYFIMRKDFAEQHGIKTLGDIVTKKLPVRFATLKPGTASEVTI
                     RALFKKGYNVGWDDFRSWGGKVEFASYDDGANLLADNHLDIFAFSVGRVASIVMQIEN
                     QVDVVLLPVEQSALDALTAAYGTETFMIEPGIYKSVTEPTPTVGDYTCVVIRKDLPED
                     LVYELSKALWANRESLALAVKDVEELDPKMALPTAVEAHPGAVKFWKELKK"
     gene            1360..3225
                     /locus_tag="PK849_06855"
     CDS             1360..3225
                     /locus_tag="PK849_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HPE65884.1"
                     /translation="MRQLRGNTQKLFYVYVVAMGLFHLYTAIFGNYEAYLQRSIHLTW
                     VLPMCFVLYPISSKSPKEYVPLYDWILAFISTLPGIYNMINYTHIIERIAQVDPLTTT
                     QLVLGTLLLVILLEATRRVVGVPLTIIAAFFAGYMYFGHHMPGIMKGLSFTFEEVIEH
                     IYLTGEGIFSVPLGVSAAFVMIFLIFGGFLEKSGVGEYFMHLAEAFTGTQAGGPAKIA
                     VVSSALFGSISGSAVANVYGTGTFTIPLMKRIGYPAHFAGAVEAVASAGGQIMPPIMG
                     AGAFIMASFLGRPYSEIMIAATLPAILYYGAVIFMVHLGALKNGLQGLEPDELPSKKE
                     VLKKLYFILPIIGLVYMLLDGYTPMWAATIGIGLTWAISMLNPGKRMTPIDAIDAIYT
                     GAKNIPMVCIACATAGIVVGSVGLTGFGFKFVGFIFAFAKDTPFVALLLVMFVSLILG
                     MGLPTTGAYILAAALGVPALAKLGFPTLSAHLFVFYFAIVSNITPPVALAAYAASSIA
                     GSSPNKTGFTAMRLGILAFIVPFAFCYDTGLLLNAGFFNNITALAGGVAATFALGYAM
                     IGFTRRPLALWQRAFFCIAGILCLSPLEISKAAGVGLLLLGLFFWRNPKPEHRIA"
     gene            3336..4022
                     /locus_tag="PK849_06860"
     CDS             3336..4022
                     /locus_tag="PK849_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="HPE65885.1"
                     /translation="MRIERFFNTSTPEIDGRKWNPYLGISIQNKAFTPEYIAEFLRWA
                     APRSAQRTAIVVVDVLQRINNQIFDRSSPMAALAKAFRKADEIHDRIRTALDSLPEEI
                     QNGVEILDWCDIIEETWFIPNYRIFKQEFEENAPFRNFLTGLTRGNLGSIIGRLQEPD
                     IEQLTRYILMELPELVTGFMHSGVHYNLNVYPGPIGSIYDEFSGQPFWPEIRDRLRFI
                     GPISSVEAYL"
     gene            complement(4108..4368)
                     /locus_tag="PK849_06865"
                     /pseudo
     CDS             complement(4108..4368)
                     /locus_tag="PK849_06865"
                     /inference="COORDINATES: protein motif:HMM:NF027074.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
BASE COUNT         1079 a         1545 c         1279 g         1048 t
ORIGIN      
        1 gtcggaaggc tgaagcgcag acagatccca ggtatactcg acaggaatat cacagcgagg
       61 agggaccttt cctccgaaag atatttctgt aaaagcggcc ttttttgctc cattgccaga
      121 ctgagtacga atcataacga aactcctttc acaaacaagg aaattttgaa aaataatgtt
      181 caaacggttt tattgacatg ccgccccgaa gactatatca tttttccgtc tgtttctcag
      241 ggaagttcta ctcaaaatct catcaaagga gtgtgtaaaa aatgaaaaga cgcgttcttc
      301 tgcttctcgc cctcgcactt gtggtctcct tttccggaac ggccttcgcg gcaaaggaag
      361 ggtggcccga tcagcttcga ttcatggcgg gacctccggg cggcaactgg ttcgccctcg
      421 gcggcgcact tgccgatatg tggtcgaagg aagtcctcca gaccaccagc atcaccggcg
      481 gcggcgttgc caacgtggtg aatactgaca atagcaaggg cgatctcggg ttttcggtaa
      541 catcgttcgt cggcgcggcg ggcaagggag aggatccctt caaggccccc gcgaaaaacg
      601 cggtcgtcat gtcgaacata tacacccagt tcacctactt catcatgcgc aaagatttcg
      661 cggaacagca cggaatcaag acgctcggcg acatcgtcac gaagaagctt cccgtacgct
      721 tcgccacgct gaagccggga accgcctccg aagttaccat ccgcgccctc ttcaaaaagg
      781 ggtacaacgt aggctgggac gatttccgca gctggggcgg caaggtcgag ttcgcctcct
      841 atgacgacgg cgcgaacctt ctggccgaca accacctcga catcttcgcc ttctccgtcg
      901 gccgcgtcgc atccatcgtc atgcagatcg aaaaccaggt cgacgtcgtt ctcctcccgg
      961 tcgaacagag cgcgctcgac gctcttacgg cagcatacgg caccgagaca ttcatgatcg
     1021 aaccgggcat ctacaagagc gtcaccgagc ccaccccgac cgtcggcgac tatacctgcg
     1081 tcgttatccg caaagacctc cccgaggatc tggtctacga gctctccaag gcgctgtggg
     1141 caaatcgtga atcacttgcc ctggctgtga aggacgtcga ggaactcgac cccaaaatgg
     1201 cgctcccaac ggccgtcgag gcgcatccgg gcgcggtgaa gttctggaag gaactcaaga
     1261 agtagctcct tcataatctc cgcactgagc gcgggcggtg gcgaatcacc gcccgctttt
     1321 taatttagaa aagaacgacc taggaagtga gtgtacacaa tgagacaact acgaggaaac
     1381 acccaaaaac tcttttacgt gtacgtagtc gccatgggac tctttcacct gtataccgcc
     1441 atcttcggca actacgaagc gtaccttcag cgaagcatcc acctgacatg ggttctcccg
     1501 atgtgcttcg tcctctatcc gatatcctcc aaatctccga aggagtacgt cccactctac
     1561 gactggattc ttgcatttat ctcgacgctg cccggcatct acaacatgat caactatacc
     1621 cacatcatcg agcgcatcgc ccaggtggat cccctgacca cgacacagct tgtcctgggc
     1681 acactgctgc ttgtcatcct gctcgaagcc acccggcgcg tcgtcggcgt tccgctgaca
     1741 atcatcgcgg ccttttttgc gggctacatg tacttcggcc accacatgcc cggcataatg
     1801 aagggcctct ctttcacatt cgaagaggtg atcgagcaca tctaccttac cggcgaagga
     1861 attttttcgg taccgctcgg tgtctccgcg gcgttcgtca tgatattcct catcttcggc
     1921 ggcttccttg aaaaaagcgg cgtcggcgag tacttcatgc accttgccga agccttcaca
     1981 ggcacgcagg caggcggccc ggcgaagatc gccgtcgtca gctcggcgct cttcggctcc
     2041 atttcgggct ccgcagtcgc caacgtttac ggaacgggta ctttcaccat ccccctgatg
     2101 aagcgcatcg gctaccccgc gcacttcgcc ggagccgtag aggccgtcgc cagcgccgga
     2161 gggcagatca tgcctccgat aatgggagcc ggcgccttca tcatggcatc cttcctcggc
     2221 cgcccctaca gcgagatcat gatcgccgca acactccccg caatactcta ctacggcgcc
     2281 gtcatcttca tggtgcatct cggcgcactc aagaacggac tgcagggcct ggaaccggat
     2341 gaactcccct ccaaaaaaga agtgctgaag aagctctact tcatccttcc catcatcggc
     2401 ctcgtctaca tgctgctcga cgggtacacg ccgatgtggg cggcaactat cggcatcggg
     2461 ctcacatggg cgatctccat gctcaacccc ggtaagcgta tgacccccat cgacgccatc
     2521 gacgcgatct acaccggcgc caagaacatt cccatggtgt gcatcgcctg cgccactgcg
     2581 ggtatcgtcg tcggctcggt cggtctcacc ggcttcggct tcaagttcgt cgggttcatt
     2641 ttcgcctttg ccaaggacac ccccttcgtc gcgttgctgc ttgtcatgtt cgtctcgctg
     2701 atcctcggca tggggcttcc gacgacaggc gcctacatcc tcgccgccgc cctcggcgtt
     2761 cccgccctgg cgaagctcgg tttcccgacg ctctccgcac acctcttcgt cttctacttc
     2821 gccatcgtct caaacatcac ccctccggtt gcgcttgccg catacgccgc aagctccatc
     2881 gccgggtctt cccccaataa aacgggcttt acggcaatgc gcctcggtat tctcgccttc
     2941 atcgtcccct tcgctttttg ttacgacacg ggactgctgt tgaacgccgg gttcttcaac
     3001 aacatcaccg cgctcgcggg aggcgtcgcc gctaccttcg ccctggggta cgccatgatc
     3061 ggcttcacga ggcgaccgct cgccctctgg cagcgcgcgt tcttctgcat tgccggcatt
     3121 ctttgcttaa gcccgctcga gatctccaaa gcggcgggag tcggtcttct gttgctgggg
     3181 ctcttcttct ggagaaaccc gaagccggaa cacaggatcg cataattatg aagaggccgc
     3241 tttcgcggcc tcttcatttt gccacatatt ccgatctcgg acgaaacgcg gcgtcaccgg
     3301 aatcgggaac ttcgtaaacg aaaggagcgc ccccgatgcg aatcgagcga tttttcaaca
     3361 cctcgactcc cgaaatcgac gggaggaagt ggaatccgta cctcggcatc agcattcaga
     3421 acaaagcgtt cactccagag tacatcgccg aattccttcg ctgggcggcg cccagatccg
     3481 ctcagcggac ggccatcgtg gtcgtcgacg ttctccagcg catcaacaac cagatattcg
     3541 accgcagctc ccccatggcg gcgttggcca aagccttccg aaaggcggac gaaattcacg
     3601 atcgaatccg gacggcgctc gattcgctcc cggaggaaat ccaaaacggc gtcgagatac
     3661 tggactggtg cgatatcatc gaagagacgt ggttcattcc gaactaccgt atcttcaaac
     3721 aggagttcga agagaacgcc ccattccgga attttttgac cggcctcacc cgcggcaatc
     3781 ttgggtcgat catcgggagg ctgcaagagc ctgatatcga gcagctcaca cggtacattc
     3841 tgatggagct tccggaactg gtcaccggct tcatgcacag tggcgtccac tacaatctca
     3901 acgtttatcc gggccccatc ggcagcatat acgacgaatt cagcggacag ccgttctggc
     3961 cggagattcg cgaccggctg cgcttcatcg gcccgatctc gagcgtcgaa gcgtatttgt
     4021 gagaggggaa aagacagcct cgttccggag cgcttccgtc tttgtattat atcgaggcct
     4081 cgatcaactg cggcaagccc gccgacctca cagtccgaga agctcggagt ggcttccgag
     4141 gcgcaccaag gtgagcgcat cttccgacag ataatagagc aacagcagat cagggacgat
     4201 gtggcgccct ctatggtcgc gccggcttcc tccaagcgag cgatccctat acgacggatc
     4261 gagagaagtc cccttcgcca gtgcgttgac gacgccccaa agacgctcct tgaggaaatg
     4321 ccgcgaccag cccttgttct cccgtttaaa atcgcgcttg aacgcggccg tttgcctata
     4381 agtagacgac cggtgaacgt gagcgaagcg aaaaaggaac caagtcggtc actcgcgtgc
     4441 ccgtagcgca gcggagggta tagtgcgaac gcttctttcg ctaacggatc gcatccagct
     4501 cggccttcag atcgtccagc gacagttctt ccgccgtgcc ggactccgct tcccgcatag
     4561 cggccagagt agcttcgggc aaagtcaggc cgaagggaat acctcccgtg ttgatggtct
     4621 gccgataaaa gagccaaatc gcctcggtgg gagtcaggcc aacttttctg aagatattct
     4681 acgcgagctc cttatcccgt gcaacaagcc gcacattgac gctgcggaat ccgggacgct
     4741 tggttttcgt aacgctcgca cctatagaaa cacatcctcc gcagagaatc ataacgccat
     4801 aagcgttaca agtaaaaagt agaccatccc gttcggctgc aataaaagac aacaaggccg
     4861 ggtgtgccga tattaaatct gtctgaaaca gttttgtaag cccttggaaa ctcagcgggc
     4921 tattgcttat tatgagcgat atcggacagc a
//
LOCUS       DAOPKU010000181         4944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18501_length_4944_cov_4.314584, whole genome shotgun sequence.
ACCESSION   DAOPKU010000181 DAOPKU010000000
VERSION     DAOPKU010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18501_length_4944_cov_4.314584"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..382)
                     /gene="ssrA"
                     /locus_tag="PK849_06870"
     tmRNA           complement(31..382)
                     /gene="ssrA"
                     /locus_tag="PK849_06870"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(403..864)
                     /gene="smpB"
                     /locus_tag="PK849_06875"
     CDS             complement(403..864)
                     /gene="smpB"
                     /locus_tag="PK849_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPE65886.1"
                     /translation="MADRTVAQNRKARHDYFVLETFECGIVLTGTEIKSVRDGKINLK
                     EGYALIRGGELWLVGVHISPYEKGSYYNHEPLRDRKLLMKHHEILRLFSKVREKGLTL
                     VPLSVYLKEGKRAKVELALVKGKLLHDKRDSLAERDAKRDIERAVRRSDRD"
     gene            complement(995..1072)
                     /locus_tag="PK849_06880"
     tRNA            complement(995..1072)
                     /locus_tag="PK849_06880"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1035..1037),aa:Pro,seq:cgg)
     gene            complement(1125..1766)
                     /locus_tag="PK849_06885"
     CDS             complement(1125..1766)
                     /locus_tag="PK849_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65887.1"
                     /translation="MTRERKNDGNEQNEIWTVGEILSAMSGSEYPQYIVKIVNQVQQW
                     MDESIRADNPEQDIDPVAALFENVMEMDGEALFNSLEWVDEYADLLPNIKKPFGNEDV
                     VALHIGPADFEDSLRMAIDYAALFNRGVSRRVWLISDTFIIGDVYRYISHIRALNRQG
                     IAFRFLLVTPWGWTEIPLAADSAPSSRIAWKNNRKGGTAPGEDDRKRDDRLTR"
     gene            2024..2581
                     /locus_tag="PK849_06890"
     CDS             2024..2581
                     /locus_tag="PK849_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPE65888.1"
                     /translation="MALLRLRRDSAHFLMIDVQERLLPAISGHEAIHRNILKMERAAA
                     VMNIPFCFTEQYPKGLGGTAAEILASLPEGALRFEKSTFSCCDEEGFNDIVDRQGRPV
                     TVLFGIETHICVLATVLDLLELNRQVIVAADACGSRLHEHAVLAMDAMRGAGALVLPV
                     ETIIYHMMGRSGTPEFKALLPLFKE"
     gene            complement(2612..3169)
                     /locus_tag="PK849_06895"
     CDS             complement(2612..3169)
                     /locus_tag="PK849_06895"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPE65889.1"
                     /translation="MTSIRAAVFKMRGGIWTVLFLAVWFLAEPPGPYRSFLGLVLVAL
                     GQMLRFWAAGCITRYRGENVGAARLTTWGPYGIVRNPLYVGNWLIGAGWGVLAGWKAF
                     FLFVLSFWILYCTIIVPYEEEYLLKTFGDEYSLYLTSTGRFFPRTFSQERLRGPFDSS
                     VLWRSERHSFYVTLAGTLLLLFFVR"
     gene            complement(3162..4247)
                     /locus_tag="PK849_06900"
     CDS             complement(3162..4247)
                     /locus_tag="PK849_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HPE65890.1"
                     /translation="MTLRKGLVLFLLLSFGVSALVLASSVDADTWRTITNADGRLLLL
                     ALVFVGAAWCCDAARFCSLARAAGEKIGFRLGMVLTWLHYFGCAVTPMQSGGGPFQVY
                     VLYKKQVPLGKGIAITLTRTLLTLLILGLVVPVAVFIEPDLLKGKLFLKGVFSYVILF
                     IVVSWVLVLLSILRPLLIKKWGRVLTLWLKRFGFVKPETVLRVVRRINLEVDNYNLNF
                     RMFFSCGLKCFLVAILFSVLHLLCIFSVLPCLIAAMGLPFHYLQALLAQAVFMFLLYF
                     VPTPGASGVAEGGGAALFGLLVPWNMAGVTAIAWRFFTEYLAIGMGVIVAVRLLGWGV
                     TEEIFHKEPAGGDEPLIKEFDDEERRD"
     gene            complement(4464..>4944)
                     /locus_tag="PK849_06905"
     CDS             complement(4464..>4944)
                     /locus_tag="PK849_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65891.1"
                     /translation="TVDGTVLEEDAFLALSGGVEARFETVPVRELVMDSLSEGTRYMS
                     HLSAGLSKVADMLEADKTAEGLDLLRQAAEGIGWSLQVMHNCQILLGISDHEVGDGKV
                     GELKTTLVQDLEKASSSIENGKHLELSFRIRTGVLPGIQRMAQYMEALLETGRKAVQ"
BASE COUNT         1293 a         1344 c         1201 g         1106 t
ORIGIN      
        1 gagcaacgac ggtgaaatac gatttcctat tggtggaggc ggggggaatc gaacccccgt
       61 ccgacaaggg ccggccgtga aagcgctacg ggcgtagttc ccgatttaat gtcggcttga
      121 aactcccggg aacaggattt cctcgcccaa gcccccttgt ctttttttcg aagccggaga
      181 cactccccga aaagcatccg cctttttgtg tcgtcttacg aaaaaacggc ggctacttct
      241 tcgtaagacg tgactgcgct aagcagccag tgcgtaatta ttattgttga cgtttgtttt
      301 tgtgtccgcg tttaacgagg ttcggacgac ctcggcccgc tctttcagac cctccgtctt
      361 gccgtcgaaa ccagtcgccc ccatttgtaa acgactgtaa cttcagtccc tatcgcttcg
      421 ccgcacggca cgctctatat ctcttttcgc gtcccgttca gcaagtgaat cccgtttgtc
      481 gtgaagaagc ttccctttca ccagggcaag ctcgactttc gccctcttac cctctttcaa
      541 gtataccgaa agaggaacca gagtcaatcc tttttcgcgc accttcgaaa aaagccgaag
      601 tatctcatgg tgcttcatca gaagttttct gtctcgcagc ggctcgtgat tgtaataact
      661 tcctttttca tacggggaaa tatgaacgcc tacaagccat aactctcctc cgcgaataag
      721 ggcatacccc tctttcaggt tgatctttcc gtcgcgcaca gacttaatct ccgttcccgt
      781 gagtacaata ccgcactcaa acgtttcaag aacgaaatag tcgtgacgtg cttttctgtt
      841 ctgcgctact gtgcggtcgg ccatcttatt ttcctcgttc tacattctag cattaaggtc
      901 agtattagtc aatacaaaaa gcaaaataca gggctcttct tatacaaata aagaggctcc
      961 ggaattccgg aacctctcgg gagaatccaa aatatggtcg gggcgagagg atttgaacct
     1021 ccgacctcct ggtcccgaac caggcgcgct taccagactg cgctacgccc cgacaccgtg
     1081 ttcccaacgt tcccggcaac ggcgaagatt gtaacacccg aaggctatct cgtcaaacgg
     1141 tcatctcgtt ttctgtcatc ttctcccgga gccgttcctc cctttcggtt gttcttccat
     1201 gcaatacggc ttgaaggcgc gctgtccgcc gcaagcggta tttcggtcca tccccaggga
     1261 gtgaccagga gaaagcgaaa tgcaatcccc tgacgattca aagcgcgtat atgagatata
     1321 taacggtata catcgccgat gatgaacgta tcgctgatca gccacactct gcggctcacc
     1381 ccgcgattga aaagagcggc gtagtctatg gccatacgaa gactgtcctc gaaatccgcc
     1441 ggaccgatat gaagcgcgac aacatcctcg tttccgaaag gtttcttgat attcggaagg
     1501 agatcggcat attcgtcgac ccattcaaga ctgttaaaga gagcctctcc gtccatttcc
     1561 atcacattct caaaaagggc ggcgaccggg tcgatatcct gctcgggatt atcggctctg
     1621 atcgactcgt ccatccactg ctgcacctgg ttgactatct ttacaatata ctgcggatat
     1681 tcagatcccg acatggcgga aagaatctct cccactgtcc agatttcgtt ttgctcgttt
     1741 ccgtcgttct ttcgttctcg tgtcatgcca tcctccgtgt ttctgatctg atggaagcat
     1801 agcacatact ccagaagctc acaacaggaa gaataaacaa aagaagtgag aaaaaagagt
     1861 ttcgcgttcg tctgttttca aaaaaagatc cttcattccg gattcgtcaa agaaattgac
     1921 tgctgaaaag agttggataa agagaaaaaa aagaccgcaa tgacagtccg ccgcatgctt
     1981 tttagtgtaa aatcgtaggg gaaaattctc aggaggtgaa atcatggctc ttcttcgtct
     2041 tcgacgtgat tcggcgcact ttcttatgat tgacgtacag gagcggctgc tccccgctat
     2101 ttcgggtcac gaagcaatcc atcgcaacat actgaagatg gaacgtgcag cagcagttat
     2161 gaacatccct ttttgcttta cagaacaata tccgaaggga cttgggggga ccgcggctga
     2221 aatcctggcg tctctgcccg aaggcgctct gcgattcgag aaaagcactt tttcatgctg
     2281 tgatgaagag gggttcaacg acatagtcga ccggcaaggc aggccagtca cggttctttt
     2341 tggtattgaa acgcatatct gcgtcctggc gacagtcctc gatctgctgg agctgaatcg
     2401 gcaagttatc gtagctgcgg atgcctgcgg aagccgtttg catgaacacg cagtccttgc
     2461 aatggacgca atgcgcgggg cgggagcgct cgttcttccg gtcgaaacca tcatctacca
     2521 tatgatgggc cgttcaggca cgccggagtt caaggcgctg ctgcctctct tcaaggagta
     2581 ggctcggggc aaaaatacga cccgttcgct gtcagcggac gaagaagagc aatagaagcg
     2641 ttccggcgag cgttacataa aaagagtgac gctcgcttcg ccacagcact gaggaatcga
     2701 aaggcccgcg caggcgctct tgagagaatg tgcgagggaa aaaacgcccg gtactggtta
     2761 gatagaggga gtactcatct ccaaatgttt ttaaaaggta ctcttcttcg taaggaacta
     2821 tgattgtaca gtagagaatc cagaaggaga ggacaaaaag gaaaaacgcc ttccaaccgg
     2881 cgagtactcc ccacccggcg cctatgagcc agttgcctac gtagagaggg ttccggacaa
     2941 ttccgtacgg cccccacgtt gtaagcctcg ccgcgccgac attctctccc cggtatcggg
     3001 tgatgcagcc tgcagcccag aatcgcagca tctggccaag agcgacaagg acaaggccca
     3061 aaaaagaacg atacggtccc ggcggctccg ctaaaaacca gacggcgaga aaaagcaccg
     3121 tccagatacc gcctcgcatc ttgaaaacag cagcccggat actagtcacg gcgttcctcg
     3181 tcgtcaaatt ctttgattaa cggttcatcg ccgccggccg gctccttatg gaaaatctct
     3241 tcggtaacgc cccatccaag caagcgcacc gctacaatga cgcccatacc gatagcaaga
     3301 tactctgtaa aaaatcgcca tgcgatcgcg gtcactccgg ccatattcca ggggacaaga
     3361 aggccgaaaa gcgccgcacc gcctccttcc gcaacgccgc tggcgccggg agtaggcaca
     3421 aagtagagta aaaacatgaa gaccgcctgc gcaagcaacg cctgaagata gtggaaagga
     3481 agccccatgg cggcaatcag acacgggaga acggagaaaa tacacagcag atgaagcacg
     3541 gaaaaaagta tcgcaaccag gaaacacttt agtccgcaag agaaaaacat gcggaagttg
     3601 agattataat tatccacttc cagattgatt cttcgcacaa cgcggagaac ggtctccggt
     3661 ttgacgaatc caaagcgctt cagccaaagc gtgagcactc tcccccattt ttttatcagc
     3721 agagggcgca gaatgctcag gagcaccaaa acccacgata caacaataaa aagaatcaca
     3781 taggaaaaaa cgccttttaa aaaaagtttc cctttaagaa gatcaggctc gataaagact
     3841 gctactggaa ctacaagccc aaggatcaaa agagtaagca gcgttctggt cagagttatc
     3901 gcaatgcctt tgccgagagg cacctgtttt ttgtaaagca catacacctg gaaagggccg
     3961 ccgccgcttt gcatcggcgt tactgcacac ccgaagtagt gcagccatgt aagcaccatt
     4021 ccaagtcgaa aaccgatctt ctcgcctgcg gcacgcgcaa gggagcagaa acgggcggca
     4081 tcacagcacc aggcggctcc gacaaagaca agcgccagca aaagcaagcg cccgtcggca
     4141 tttgttatcg tccgccacgt atcggcatcg acgcttgaag cgagaacaag cgcgctgacg
     4201 ccaaaagaaa gaagcagaaa cagaacgagt cctttacgaa gggtcaatac agaagtgcct
     4261 cctcacaggt tccggctgcc ccgcagctct ccgcgccagg gcggatataa tgcggaatcg
     4321 ggggatagtg tagcagattt tttcgtctag aaaagcgttc ttaaaaagaa aacgaccgct
     4381 ctccggatca acactcaaag aatgttttcc ggcccgttat cacggagacg tcaaaaaagt
     4441 tatgcctggc gttttgaaaa gagttactgc actgcttttc ggccggtttc caaaagagct
     4501 tccatgtact gcgccattct ctgaattcca ggaagcacgc ctgtacggat acgaaacgaa
     4561 agctccagat gtttcccgtt ttcgatggat gaggatgctt tttccaggtc ctgtacaagg
     4621 gtcgttttga gctcgccgac ctttccgtcg cccacttcgt ggtcagagat tcccagaagg
     4681 atttgacagt tatgcatcac ctgtaaagac catccgatac cttcagcggc ctgccgtaaa
     4741 aggtccagac cttcggctgt tttatcggct tccagcatat ccgcaacctt ggaaagccct
     4801 gcggaaagat ggctcatgta ccgagttccc tcggaaagcg aatccatgac aagttcccgt
     4861 accgggacgg tttcaaaccg ggcttccact cctccggaga gggctaaaaa agcatcctcc
     4921 tctaaaactg ttccgtcaac ggta
//
LOCUS       DAOPKU010000182         4940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18512_length_4940_cov_5.124872, whole genome shotgun sequence.
ACCESSION   DAOPKU010000182 DAOPKU010000000
VERSION     DAOPKU010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18512_length_4940_cov_5.124872"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PK849_06910"
     CDS             <1..519
                     /locus_tag="PK849_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65892.1"
                     /translation="YGASFGGYFALSGAVGEPRLGAVIANSPLIDAQEHFFAGMGPHF
                     VSMFQNQDLTAEEIRAMPEEELPAKYKFSLLSLCVRYGKPSVKETLDVMRDFRLREDQ
                     LRSLNIPALGLVSEAEGTVPLAQAKRFAELAQKAALHIFTEESGANIHCQLDNMPLSW
                     AVALDWLDEQFR"
     gene            complement(629..1495)
                     /gene="lsrF"
                     /locus_tag="PK849_06915"
     CDS             complement(629..1495)
                     /gene="lsrF"
                     /locus_tag="PK849_06915"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HPE65893.1"
                     /translation="MFEHMSWGKKNRLSSIVAPDGRALMLAIDHGYFMGATHGMEKPA
                     EAIEKLLPHVDSLMLSPGILSTCIDPALTQGVVLRASGGNSILDPDIDDEELILTAAQ
                     AVRMNASAVAVSIFVGSEHGHQTILNLTDMISQAAEFDLPVLGVTGVGKALKDRKEKR
                     YLSHAARLCAEMGADIVKTYYCEGFAEVVAKTPAPIVVAGGPKLDTYRDVIELTYKAM
                     QEGAIGVDMGRNVWQSDYPTAILQGVKAVIHGNASVKDAMEIVGELSTPENARKEYFK
                     VTAEDIANSAVH"
     gene            1701..2693
                     /locus_tag="PK849_06920"
     CDS             1701..2693
                     /locus_tag="PK849_06920"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HPE65894.1"
                     /translation="MQERPRDENSPRRQYLAEVAHLYYNQGKTQQEIAQLVGITRSGV
                     SRLLTEAREKGIVEILVHYPWQTSKALEDELSELFGLKDVRVLVRGGKNDREMLDGLG
                     SVAAQFFLNILRDGHIVGVSWGTALYSMLQAIRPVHRPDVKVVQLIGGTGAEHSSIVG
                     PLLAPMLANLLGCSCHYLHAPLITESEIFRKAVLQERPIRETLELGRKANIALVGIGA
                     THPEIYNPFRLGYISKDELNEISGNGAVGLICGRHYSIDGTELDIQVNRKVIGITLKE
                     LEEVEWVIGVAGGALKAESILGALRGRHVNVLVTDDIAARKALEIHKAGGSRAV"
     gene            complement(2697..3860)
                     /locus_tag="PK849_06925"
     CDS             complement(2697..3860)
                     /locus_tag="PK849_06925"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPE65895.1"
                     /translation="MVESSVYRIPAVDLITFCGVVAEDVLSPHGALLLSRDADFAALS
                     PSKRRGFAFSLLNAGVSSVAVRERPELSLNEILEVMARGKRPPEQRFRNLYEDAMYYL
                     TSFCEKVFSGGPGTLQSFTLLRAGRMLAEAVAADPDIPLHLGFVLRKDNLLLSHSINV
                     GLLSAFLAHRMFPDREMTAVSVAAGGLLHDLGKTYISEEILNKPGALSEKEYETIRRH
                     PLVGEAALRKSGVTDSVMLKMVRNHHERMDGTGYPDGLSGESIPIAARIAAVADVFDA
                     VTSERAYKKRMSGRKGVSLLMDSIGTHLDSEVVRVFVGAFGMYPPGAEVVLSDGRSGV
                     VVAPGERGVLRPRVLLHRDERGDELSSAVFLDLAAAPALFIRRCAGEDVPEAV"
     gene            4245..>4940
                     /locus_tag="PK849_06930"
     CDS             4245..>4940
                     /locus_tag="PK849_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPE65896.1"
                     /translation="MRVAVLVKQVPATENVKIDETTGTMIREGVEAELNPLDLHAVEE
                     AVRLKERFEGTEVVVISMGPPQAKKAVKAAIAMGCDRGVLLSDRKFAGSDTLATARTL
                     AAGLVHEGGFDLALSGERATDGETGQVGPAVGRILDMSALTFVSEVESVGAEKIVVRR
                     AVEGGHERVEAPLPAMLSVVKEINVPRLCTLGGKIRAKKAEIPVVGAAELAIPEEHLG
                     LAGSPTKVVRVSYP"
BASE COUNT          990 a         1490 c         1525 g          935 t
ORIGIN      
        1 tacggggcca gtttcggcgg ctacttcgcc ctctccggcg cggtcggcga accccggctg
       61 ggcgcggtaa tagcgaactc gccgctcatc gacgcgcagg aacatttctt cgccggcatg
      121 ggaccgcatt ttgtttccat gtttcagaat caggatctca cggcggagga aatacgcgcc
      181 atgccggagg aggagctgcc cgcgaaatac aagttctccc tgctgagcct ctgcgtgcgc
      241 tacggcaaac cttcggtgaa ggagacgctc gacgtcatgc gggacttccg gctgcgcgaa
      301 gaccagctcc gctcgctgaa catacccgcg ctgggactcg tcagcgaagc ggagggaaca
      361 gtcccccttg cgcaggcgaa gcgattcgcg gagctggctc aaaaggccgc gctccacatc
      421 ttcacggagg agagcggcgc gaacatccac tgccagctcg acaacatgcc cctctcgtgg
      481 gcggttgcgc tcgactggct ggacgagcag ttccgatagg cgcaacaaca aaggaacggg
      541 gtgggagccg ccgaaaaagc gctctcgccc cgttctttta tagtcgttgc atttccgggc
      601 ggattcgcgc cgcgaggaga ataaccgttt aatgcaccgc gctgttcgcg atgtcttcgg
      661 ccgtgacctt gaagtactcc ttccgcgcgt tttccggcgt ggaaagttcg cccacgatct
      721 ccatggcgtc cttcaccgac gcattgccgt ggatgacggc cttcacgccc tgcagaatcg
      781 ccgtcgggta gtccgactgc cacacgttgc ggcccatgtc gacgccgatg gcgccttcct
      841 gcatcgcctt gtaggtgagc tcgatcacgt cgcggtaggt atccagcttc gggcctccgg
      901 cgaccacgat cggggcgggc gtcttcgcca caacctccgc aaacccctcg cagtaatagg
      961 tcttgacgat atcggcgccc atctccgcgc acagccttgc ggcgtgcgag aggtagcgtt
     1021 tctccttgcg gtccttcagc gccttcccca ctccggtgac gccaagcacc gggagatcga
     1081 actccgctgc ctgggagatc atgtcggtga ggttcaggat cgtctgatgg ccgtgctcgg
     1141 agccgacgaa gatggagacg gcgaccgccg aggcgttcat gcggacggcc tgcgcagcgg
     1201 tgaggatcag ctcctcgtcg tcgatgtcgg ggtcgagaat gctgttgccg ccgcttgcgc
     1261 gcaagaccac gccctgcgtc agggcgggat cgatgcaggt ggagagaatt cccgggctca
     1321 gcatcaggga atcgacgtgc ggaagcagct tctctatcgc ctcggccggc ttctccatgc
     1381 cgtgcgtggc gcccatgaaa tagccgtggt cgatggcgag catcagcgct ctgccgtcgg
     1441 gagccacgat ggaactcagt ctgttcttct ttccccagct catatgttca aacatagcgt
     1501 tcctccttgt gacaaaaaga tttcacgtat gtgaaattca atcacgaaag tgatatactc
     1561 tgcatgtcat tctaacgtat gtccctgtat tgtcaagaaa aaaactacaa atttcttgag
     1621 acgtcttttc acttttattc tgctctattc tggtacaatg cagcgctata cacggagaga
     1681 catccgagga ggggaagagc atgcaggaac gcccgaggga cgagaacagc ccgagaaggc
     1741 agtatctcgc cgaggtggct catctgtact ataaccaggg gaagacgcag caggagatcg
     1801 cgcagctcgt gggcatcacg cggtcggggg tttcgcggct gttgacggag gcacgcgaga
     1861 aggggatcgt cgagatcctc gtccactatc cgtggcagac ttcgaaagcg cttgaggacg
     1921 agctttcgga gcttttcggg ctgaaggacg tccgcgtcct cgtccgggga gggaagaacg
     1981 accgcgagat gctcgacgga ctgggttccg tcgcggcgca gttcttcctg aacattctgc
     2041 gggacggcca catcgtcggc gtttcgtggg gaacggccct ctacagcatg ctccaggcga
     2101 tccgccccgt gcaccggccg gacgtcaagg tggtgcagct tatcggaggc acgggggccg
     2161 aacactcgtc catcgtcggc ccgctgctcg cgccgatgct ggcgaacctc ctcggctgtt
     2221 cgtgccacta ccttcacgcg ccgctgatca cggagagcga gatatttcgc aaggcggtcc
     2281 ttcaggaacg cccgatccgc gagacgctcg aactcggacg aaaagcaaac atcgccctgg
     2341 tgggcatagg cgcgacgcac ccggagatct acaacccctt ccgcctgggg tacatttcca
     2401 aggacgagct gaacgagatc tccggcaacg gcgcggtcgg gctcatctgc ggccgtcact
     2461 actccatcga cggcacggag ctggatattc aggtgaacag aaaagtgatc ggcattacgc
     2521 tgaaggagct ggaagaggtt gagtgggtga tcggcgtcgc ggggggcgcg ctcaaggcgg
     2581 agtcgatcct gggagcgctc agggggcggc acgtgaacgt cctcgtcacc gacgacatcg
     2641 ccgcgcgaaa ggcgcttgag atccacaagg ccgggggatc gcgggcagtt taaggatcag
     2701 acggcctcgg gaacgtcctc tccggcgcat cttcgtataa agagcgcggg cgcggccgca
     2761 aggtcgagaa agacggcgga agaaagctcg tctccgcgct cgtcgcgatg caaaagcacc
     2821 ctggggcgca gcaccccccg ttctccgggg gcgacgacga ctccggaccg tccgtcggag
     2881 aggacaacct cggcgccggg cgggtacatc ccgaaagcgc cgacgaacac cctcaccacc
     2941 tcgctgtcga gatgcgttcc tatgctgtcc atcagcagcg aaacgccctt ccttccggac
     3001 atacgtttct tgtaggcgcg ctccgacgtc accgcgtcaa agacgtcggc cacggccgca
     3061 atcctcgccg ctatcgggat ggactcgcct gaaagcccgt ccggataccc cgttccgtcc
     3121 atccgttcat gatggttccg caccattttc aacataacgc tgtccgtcac gcccgacttc
     3181 cgcagcgcag cctcccccac gagcgggtgt ctgcgtattg tttcgtactc tttttccgaa
     3241 agagccccgg gcttgttcag aatttcctcg gagatatacg tcttgccaag gtcgtgcaac
     3301 agtccgcccg cggcgacgga aacagccgtc atctccctgt cggggaacat cctgtgcgcc
     3361 agaaacgccg agagcagccc cacgttgatc gagtgcgaga gcagcagatt gtccttcctg
     3421 agcacgaatc ccaggtgcag cggaatatcg ggatcggcgg cgacggcttc ggcaagcatt
     3481 cgccccgcac gaagcagcgt gaagctctgc agcgtgcccg gtccgccgga gaacaccttt
     3541 tcgcaaaagg aggtcagata gtacatcgca tcctcgtaca aattgcgaaa gcgctgctcc
     3601 ggggggcgtt tcccgcgcgc cataacctcc agaatttcgt tcagcgagag ctccggccgt
     3661 tcgcgtaccg cgaccgaaga aacccctgcg ttcaggaggg aaaaagcgaa tcctctccgc
     3721 ttggaaggag aaagcgccgc gaagtcggcg tcccttgaga gaagcagcgc gccgtgcggg
     3781 gagagcacat cttcggcaac aacgccgcaa aaggtgatga gatcgactgc cggaatccgg
     3841 tatacactgc tttccaccat cgccatgccc tcctgtgagc gtgagatgca aatcgaaaga
     3901 aaaggactat accccatgca cgatgcatag agagagagag ccgtttgagt ataccatgct
     3961 gcgactgccc tccccgcgct gcgagcaaca ggaacagtgc tccttgcgct tcatttccgc
     4021 gaagcggcgc gcccttgtct cagtcgaaag actcccatga acaccaggta cccggaagca
     4081 tttgcgcctt catacccctt acaaaagaag tacctttgat aaataagtcc tgttttatat
     4141 gtatttttta tcattatata aataatagaa tcgtcttgct ttatgaaaat ttagtggtag
     4201 gatatcttct tatttcatgt tcattttttc gggaggtgcg tcgggtgcgc gttgctgttc
     4261 ttgtgaaaca ggtccccgcg acggaaaacg tcaagatcga cgagacgacg ggaaccatga
     4321 tacgcgaggg tgtggaggcc gagctgaatc cgctcgatct gcacgccgtg gaggaggcgg
     4381 tccggctcaa ggagcgtttc gaggggaccg aggtcgtcgt gatctccatg gggccgcccc
     4441 aggcaaaaaa agcggtgaaa gccgccatcg cgatggggtg cgacaggggc gtgctgctct
     4501 ccgacagaaa gttcgccggt tcggacacgc tggccacggc gcggacgctc gccgcggggc
     4561 tcgtccacga gggggggttc gacctggcgc tctccggcga acgggcgacc gacggcgaaa
     4621 ccgggcaggt cggtccggcg gtcgggcgga tccttgacat gtccgcgctc accttcgtca
     4681 gcgaggtgga gtcggtcggc gcggaaaaga tcgtcgtccg ccgcgcggtc gaggggggac
     4741 acgaacgtgt ggaggcccct ctccccgcaa tgctctccgt ggtgaaggag atcaacgtcc
     4801 cgaggctctg cacgctcggc gggaagatcc gcgcgaagaa ggcggagatc cccgtcgtcg
     4861 gggcagccga acttgcgatt ccggaggagc acctcggctt ggcgggatcg cccacgaagg
     4921 tggtcagggt gtcgtacccg
//
LOCUS       DAOPKU010000183         4938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18530_length_4938_cov_4.609257, whole genome shotgun sequence.
ACCESSION   DAOPKU010000183 DAOPKU010000000
VERSION     DAOPKU010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18530_length_4938_cov_4.609257"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PK849_06935"
     CDS             complement(<1..693)
                     /locus_tag="PK849_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPE65897.1"
                     /translation="MKPENLVKNAIGREVPTSVQGLGEFTPYAGPRARLDGKHLWTPT
                     LYPRKIAAPTTNKVAHSLKEAIEKSGLRDGMTISFHHHLRNGDALVGMTLSLLDEMGI
                     RNITLAPSSLGDSHDCVADAVRKGVVTRLNTSGVRGEVGKAISNGEFELPVMIRSHGG
                     RARAVEEGSLHIDVAFLAAPACDRLGNFTGSAGKTACGSMGYAMLDARYADHVIAVTD
                     NLAEYPLAPRISV"
     gene            complement(690..1589)
                     /locus_tag="PK849_06940"
     CDS             complement(690..1589)
                     /locus_tag="PK849_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPE65898.1"
                     /translation="MTTFLDRPRRSLLYIPGNNPGMIQNCAIYGSDGVLLDLEDSISI
                     SEKDSARKLVKHALKTLDFGPVERVVRINGLDTPFFRDDLEEIVPSRPDAVRIPKIDS
                     PDDIQTADEIITRLEREHGMEPGSVRIHAMLETARAIVNAPSIAASSPRILGLTIGGQ
                     DLAADLGIKATREGLEMLYAKSAVILAAKANGLLAFDTVYTDINNQEGLREQAKMSVS
                     LGFSGKAAIHPSQIAIIHEAFSPDEKEVRKAERIVRGAKEAEERGLGVVAVDGRMVDA
                     PVVAQAVRTLELARLAGMEVEIL"
     gene            complement(1586..3073)
                     /locus_tag="PK849_06945"
     CDS             complement(1586..3073)
                     /locus_tag="PK849_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPE65899.1"
                     /translation="MDAIFQNLAIGFSTAITAYNILWVFIGGLLGTVMGMLPGLGPAT
                     GVAILIPITYGMNPSTALITMCAVYYGAMFGGSRASIMINTPGDASAIVSCFDGYPMT
                     KNGEAGAALAISAIASFIGGTIGMLFLVFLTGPIADAALRFGPAEMFSLMLFALVATI
                     TLAEGSLLKGVIAMCIGFMLSTIGIDALTGILRFTYGVEELQDGIDFLVVMIGVYAIC
                     EVFKNLKNLETKYNLDSKSIGKVWVSWPQFKKCIMPMLRSSPLGFFVGVLPGMGASIA
                     TFMSYAMEKNLSKTPEKFGKGAIEGLAAPEAANNSCAAGAMVPLLTLGIPGSGTTAVM
                     LGALMMLGVKPGPILFSQYPEIAWGVIASILIGNIILVIINLPLAIPLVQLLKLPQRV
                     MMPLILGMAFMGTYFLNYSSFDFILVMGFALGGYIFSKLEIPLPPMILALILGGTTEQ
                     SFRNSMSLSDGSLGIFFSKPISVTLIALAGLSLVYSLLKRRKHAQ"
     gene            complement(3087..3560)
                     /locus_tag="PK849_06950"
     CDS             complement(3087..3560)
                     /locus_tag="PK849_06950"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HPE65900.1"
                     /translation="MSKNFMSGLLSLVLGAIYFFTATQFPEVTAGDRTGPKLFPLIIG
                     SVAMIAGGLLCVLDRRSGKGQPIEWNFVADRAVWSKILVIMLLGISYGLVLDSWGYLV
                     ATTVFMFCITLLINRRHFLQNLLISIFFPVVTYAAFAVALQLSLPRGIIENMLPF"
     gene            complement(3646..4620)
                     /locus_tag="PK849_06955"
     CDS             complement(3646..4620)
                     /locus_tag="PK849_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HPE65901.1"
                     /translation="MKKLFAVVLLVVLFAVAGTASAEWKPSRTIELIAPANPGGGWDM
                     LCRTVQKALTDEKLVEKNVIVVNKPGGGGATGWNYLKGKKGQGEYLSATSTLLMLNNL
                     LGKSEITYKDVTPIAALQTEWISIAVEQDSPWKTVKDLFEAIKADPSSVPVGVGPTLG
                     NNDHLMFLELAQEFGVEPASIKFIVYPGAGGEIVPALLGGHVKATTIGLGEVLEQHKA
                     GKMRIIGVSADQKLDFLPDVDTFTAQGVNLVFPHWRGIIGAPDLTPEQVKYWDDVFTK
                     MVQTETWKTLIANLGWSNFYQNSAEHTTFLEKSTTDFDKLLTQVGLKK"
     gene            complement(4688..>4938)
                     /locus_tag="PK849_06960"
     CDS             complement(4688..>4938)
                     /locus_tag="PK849_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65902.1"
                     /translation="SERLAELVWAQRELFDRYMVMRIILPNRINKSLSRRKLLCEALS
                     AHDPERAASVWTEHLDESFVIWREKSGYAKELEDFQFF"
BASE COUNT          951 a         1430 c         1571 g          986 t
ORIGIN      
        1 aaccgagatg cgcggcgcga gcgggtactc tgcaaggttg tcggttacgg cgatcacatg
       61 atccgcgtag cgcgcgtcga gcatcgcgta ccccatcgag ccgcaggccg tctttcccgc
      121 cgaaccggtg aagttgccga gcctgtcgca ggccggcgcc gcgaggaagg cgacgtcgat
      181 gtggaggctt ccctcctcga cggcgcgcgc acgccctccg tggctgcgaa tcatcacggg
      241 aagctcgaac tcgccgttcg atattgcttt gccgacctct ccacggacgc cagatgtgtt
      301 caggcgcgtg acgactccct tacgcacggc gtcggcgacg cagtcgtgcg agtcgcccag
      361 cgaactcgga gcgagcgtga tgttccgtat gcccatctcg tcgagcagcg agagtgtcat
      421 cccgacgaga gcgtcgccgt tcctcaagtg atgatggaag gagatcgtca tgccgtcgcg
      481 gagtcccgac ttctcgatcg cttccttgag gctgtgcgcc accttgttcg tcgtcggcgc
      541 ggcgattttt ctggggtaga gcgtcggcgt ccagaggtgc ttcccgtcga gccgtgcgcg
      601 cgggcccgcg tagggagtga actctccgag cccctgaacg gacgtcggga cctctcttcc
      661 gatggcgttc ttcacaagat tctccggttt cataggatct cgacctccat tcccgcgaga
      721 cgggcaagtt cgagcgtccg cacagcctgc gcgacgacgg gcgcgtccac catgcggccg
      781 tcgacggcca cgacgcccag tccgcgctct tctgcttcct ttgccccccg gacgatgcgc
      841 tcggccttcc ggacctcttt ctcgtccggg gagaacgctt cgtgaatgat cgcgatctgc
      901 gaggggtgaa tggcggcctt gccggagaag cccagagaga ccgacatctt tgcctgttcg
      961 cggagtcctt cctggttatt gatgtcggtg tagaccgtat cgaacgcgag cagaccgttc
     1021 gccttcgcgg cgaggatgac ggcgctcttt gcgtagagca tctccagccc ttcgcgggtc
     1081 gccttgatgc cgaggtcggc ggcgagatcc tgcccgccga tggtgaggcc gagaatacgg
     1141 ggggaggacg cggcgatcga gggagcgttc acgatcgccc gcgccgtttc gagcatcgcg
     1201 tggatgcgca ccgagccggg ctccatgccg tgctcccgct ccagccgtgt gatgatctcg
     1261 tcggcggttt ggatatcgtc cggggagtct atcttgggaa ttctcacggc gtccgggcgc
     1321 gagggaacga tctcttcaag gtcgtccctg aagaaggggg tatccagccc gttgatgcgc
     1381 acgacgcgct ccaccgggcc gaagtcgagc gtcttcagcg cgtgtttcac cagcttgcgg
     1441 gccgagtcct tctcggatat ggagatggag tcctccaggt cgaggaggac tccatccgaa
     1501 ccgtagatgg cgcagttctg gatcattccc ggattgttcc ccggtatgta aagcagggac
     1561 cgccggggac ggtccaaaaa tgtagtcatt gggcatgctt ccttcttttc agcagagagt
     1621 agacgagcga gaggcccgcg agtgcgatga gcgtcacgga gatcggtttc gagaagaaga
     1681 tgccgagcga accgtccgag agcgacatcg agttccggaa cgactgttcc gtggtgcctc
     1741 ccaggataag agccagtatc atcggaggca gcggtatttc gagtttggag aagatgtacc
     1801 cgccgagggc gaaccccatg acgaggatga aatcgaagga gctgtaattc agaaagtacg
     1861 ttcccatgaa ggccatgccc aggatgagcg gcatcataac tctctgggga agcttcagga
     1921 gctgcacaag aggaatggcc aacggcagat tgatgatgac gaggatgatg ttgccgatga
     1981 gaatggaggc gatgacgccc cacgcgatct ccggatactg ggagaagaga atcggcccgg
     2041 gcttgacgcc gagcatcatc agcgcgccga gcatgaccgc cgtcgttccg gagccgggga
     2101 tgccgagcgt gagcagcggc accatcgcgc ccgccgcgca ggagttgttc gccgcctcgg
     2161 gcgccgcgag tccttcgatg gcccccttgc cgaacttctc cggcgtcttg ctcaggttct
     2221 tctccatagc gtaggacatg aaggtggcga tgctcgcgcc cattcccggg agcacgccga
     2281 cgaagaagcc gaggggagag ctgcggagca tgggcatgat gcacttcttg aactgcggcc
     2341 agcttaccca caccttgccg atgctcttcg agtcgaggtt gtacttcgtc tccagattct
     2401 tcaggttctt gaagacctcg cagatggcgt agactccgat catcaccaca agaaagtcga
     2461 tcccgtcctg cagctcctcg acgccgtagg tgaagcggag gatgccggtc agcgcgtcga
     2521 tgccgattgt cgaaagcatg aagccgatgc acattgcgat gacgcccttc agaaggctgc
     2581 cttcggcgag cgtgatggtg gcgacgagcg cgaagagcat cagcgagaac atctccgcgg
     2641 ggccgaaacg gagcgcggcg tccgcgatgg ggccggtgag gaaaaccaga aaaagcatgc
     2701 cgatcgtccc gccgatgaac gaagcgatgg ccgagatggc aagcgccgct cccgcctctc
     2761 cgttcttcgt catggggtag ccgtcgaagc aggagacgat ggcggaggcg tcgcccggcg
     2821 tgttgatcat gatggaagct cgggagccgc cgaacatcgc gccgtagtag acggcgcaca
     2881 tcgtaataag cgccgtcgag gggttcatcc cgtaggtgat ggggatgagg atggcgacgc
     2941 cggttgcggg cccgaggccg gggagcatgc ccatgactgt gccgagaagg cctccgatga
     3001 agacccacag tatgttgtag gccgtgatgg cggtggagaa tcctattgcg agattttgaa
     3061 agatagcgtc catgtgtgcc gacctcctag aaggggagca tgttctcgat gatgccgcga
     3121 gggaggctca gctgaagagc cactgcgaag gccgcgtatg tgaccacggg gaaaaagatg
     3181 gagatgagaa gattctgcaa gaagtgcctc cggttgatga gcagcgtgat gcagaacatg
     3241 aaaaccgttg tggcgacaag gtatccccaa gagtcgagga caagcccgta agagattccc
     3301 agcagcatga tgacgagaat cttcgaccac accgcgcggt ccgcgacaaa attccactcg
     3361 atcggttgcc cttttccgct tcttctgtcc aggacgcaga gaagccctcc ggcgatcatg
     3421 gcgacgctcc cgatgatcag agggaagagc ttcggccctg ttctgtctcc tgccgtaact
     3481 tcaggaaact gcgtcgccgt aaaaaaatag attgctccca ggacgagaga gagcagtccg
     3541 ctcatgaagt ttttgctcat tctgcaacct ccgttttctg ttgtttcttc agaaaagaaa
     3601 gacggcggga aaatccgtcg atctccccgc cgaaaggaat acgagctact tcttcagccc
     3661 aacctgcgtc agcagcttgt cgaagtccgt tgtgctcttc tcgaggaagg tcgtatgctc
     3721 ggcggagttc tggtagaagt tgctccaccc gagattcgcg ataagggttt tccaggtctc
     3781 ggtctgcacc atctttgtaa agacgtcgtc ccagtacttc acctgctccg gcgtcagatc
     3841 gggagcgccg atgatgcctc tccagtgcgg gaagacgaga ttgacgccct gcgctgtgaa
     3901 ggtgtcgacg tccggaagga agtcgagctt ctggtcggcg ctcacgccga tgatccgcat
     3961 tttgccggct ttgtgctgtt caagaacttc gccgaggcct attgtcgtgg cctttacgtg
     4021 gccgccgaga agcgccggga cgatctcgcc gcccgcgccg gggtagacga tgaacttgat
     4081 gcttgcgggc tcgacgccga actcctgggc gagttccagg aacatcaggt ggtcgttgtt
     4141 gccgagtgtg gggccgacgc ctacgggcac ggaggacgga tcggccttga tggcctcgaa
     4201 gagatctttc acggtcttcc agggggagtc ctgctcgacc gcgatggaga tccactccgt
     4261 ctgcagagcg gcgatggggg tgacgtcctt gtaggtgatc tcgctcttgc cgagaaggtt
     4321 gttgagcatg agcagcgtgg acgttgcgga gaggtactcg ccctgcccct tcttgccctt
     4381 caggtagttc catccggtgg cgccgccgcc gcccggtttg ttgacgacga tgacgttctt
     4441 ttcgacgagc ttctcatcag tcagcgcctt ctggacggtg cggcacagca tatcccatcc
     4501 tccgccgggg ttggccggag cgatgagttc gatggtgcgg gagggcttcc attcggcgga
     4561 ggcggttccc gcgacggcga acaacacgac gagcaacaca actgcaaaca gcttcttcat
     4621 acctttgttt ccccctctgt actaggattt ttgctactcc gctccttatt gaagagcggt
     4681 accagcccta aaaaaactga aaatcctcca gctccttcgc ataccccgac ttctcgcgcc
     4741 agatgacgaa cgactcgtcg agatgctccg tccagacgga ggccgctctc tccgggtcgt
     4801 gcgccgaaag cgcttcgcag agcagtttcc ggcgggagag gctcttgttg atcctgttgg
     4861 gaaggatgat ccgcatgacc atgtagcggt cgaagagttc gcgctgagcc cagacgagct
     4921 cggcaagccg ttcggacc
//
LOCUS       DAOPKU010000184         4923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18598_length_4923_cov_3.871611, whole genome shotgun sequence.
ACCESSION   DAOPKU010000184 DAOPKU010000000
VERSION     DAOPKU010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18598_length_4923_cov_3.871611"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1812)
                     /locus_tag="PK849_06965"
     CDS             complement(244..1812)
                     /locus_tag="PK849_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPE65903.1"
                     /translation="MENNQQPRKRGIFESFIKGIEIVGNWLPHPFWLFVLLSLIVVGL
                     SYYLGNEGVAVKYMAAKAGEAPKEVTVAVENLLSFKYMRGFMADFVKTYVNFAPLGLI
                     VVMTLGIGLVEQSGMISALMRKTILGAPSYLVTAVLAVVGINANLASDAGIIFTPAIG
                     GAVFKALGRNPWIGVIAGFAAASGGFTANFFIAGTDALLAGITESAAKGMNVAGPTHP
                     LINWYFMAVATIVVMVVTTFVTEKFTVKILGDTTHDKDSEELLKHKVTAEENRGLRWA
                     AVMGVLCIGVLLYLTLPEGSFFRADDGSIVPRSPFLSGIVGILFFLFFFVGIAYGFGA
                     GTIKKLDDVPKLMQKGLSGGLSFMVVVLPAAIFVQLFNASKLTTILAVNGAHWLERMN
                     LGGIPLLVMFILLCTFINLFITSGSAKWLILAPIFVPMFSIVGFTPALTQVAYRIGDS
                     SSNIISPLSYYVPVVIGLLEQYRTDPDTKIGIGTVVSLEMPYTIAYLICFTALLIVWY
                     TLGLPLGPGAPALM"
     gene            complement(1924..3213)
                     /locus_tag="PK849_06970"
     CDS             complement(1924..3213)
                     /locus_tag="PK849_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE65904.1"
                     /translation="MKTTMDSSMKQRLVQLRREFHAHAETGWTEFWTTAKIAGILKNL
                     GYQVSVGKEILDLESVMGRPSEDEMAKYIERAVHQGADPEWIKRMDGYTGAVAVLDTG
                     KKGPTLAFRFDIDAVDVAEAAEENHRPFREGFASMNENAMHSCGHDAHTVIGLGFAEL
                     LMRRKEELKGVFKLIFQPAEEGVRGGKAVAMKGILDDVDYFLGAHIGMGIPTGNVAPG
                     CGDFLCTTKFDAVYKGKAAHAGGAPNEGKNALLAAASATLALAGISPHKDGATRLNVG
                     VLHAGTGRNVVPSKAVMKVETRGLTTALNEYVYGRAMEIVEGAAVMYGVELSVAKMGE
                     AVDAFSDKELVDLVAEKALSVPGIEKLAPSVSLGGSEDVSWMMRAVQQHGGKAVYFVL
                     GSDIAAGHHNEFFDVDERVLSYGAALFADLAFALSER"
     gene            complement(3210..4415)
                     /locus_tag="PK849_06975"
     CDS             complement(3210..4415)
                     /locus_tag="PK849_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE65905.1"
                     /translation="MNEKIRSLTKKYEEKMLELRRRIHKHPELSGEERETAALVASVL
                     EESGVEVQSDVGGHGVVGVLRGSAKGPCVALRADMDALPMEEKTGLPWASEVKGIMHA
                     CGHDSHTAMLLGAMLVLAEMKNELPGTVKCIFQPAEEANPVGGAPAMIQQGALKAPEV
                     DAIFGMHVWPSLVTGTIGTRSGALMGASDRLFLTVRGKGAHGSEPENGVDAIAIAAQI
                     LTALQTIVSRSVSPLDAAVVSVGTIKGGNRYNVIADEVLLEGTVRTINPETQEKMPGR
                     IEALVRGIARGMGGDCDMEYVKGYPPLMNDPELAKTAFSVLKKVVGEEKIVYVEKPAL
                     GGEDFAFFSKEIPALFLWLGCRPEGVAREDMPPLHNNMFLPDEKALSLGVETLVSCAL
                     EYLEGRKGA"
     gene            complement(4658..>4923)
                     /gene="speD"
                     /locus_tag="PK849_06980"
     CDS             complement(4658..>4923)
                     /gene="speD"
                     /locus_tag="PK849_06980"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPE65906.1"
                     /translation="DGVSGVVVISESHLSIHTWPEVGYMALDIYTCGEESKPQIAVDY
                     VLEQIGAKHTHITEITRGLDDGDMEYYHSFVTWQEVLQRNSNN"
BASE COUNT         1201 a         1413 c         1195 g         1114 t
ORIGIN      
        1 gtccctatgt ggaagagatg ccgctctatc ggccgcttga gtgggaacgg accgaaaaga
       61 tcctgagcag agccgtcatg ttcgggctca acatcgtaat ggacgacgcc agggtggaaa
      121 agttgatcac ggcgattaaa gcaggagcaa aagccgccct ttaatctgaa accacaagca
      181 caagaaagcc tccgcgatat gcggaggctt tcttgttttt cttccttgtt gaccgggact
      241 ctcttacata agagcaggcg cgccggggcc aagaggcaga ccgagcgtgt accacacgat
      301 caacagcgcg gtgaagcaga tgaggtaggc aatggtatag ggcatttcca gtgaaaccac
      361 cgttccgata ccgatcttcg tatccggatc ggtacggtac tgctcaagaa gtcctatgac
      421 aaccggaacg taatacgaaa gcggagaaat gatgttcgac gaagaatcgc cgatacggta
      481 ggcaacctga gtcagcgcag gggtgaaccc cacgatggag aacatcggaa cgaatatcgg
      541 cgcgaggatc agccacttcg cagagccgct ggtaatgaag aggttgatga acgtgcaaag
      601 gaggataaac atgacaagaa gcggaatacc tccgaggttc attcgttcga gccaatgggc
      661 gccgttcacc gcgaggatcg ttgtcagctt gcttgcgttg aaaagctgaa caaagatagc
      721 cgcaggtagc acaacgacca tgaaactcag accgcctgag agtcccttct gcatgagttt
      781 gggcacatca tccaacttct tgatggtacc ggcgccaaaa ccgtaggcaa taccgacgaa
      841 gaagaacagg aagaaaagaa tgcctacgat gccgctcagg aagggagaac ggggaacgat
      901 actcccgtca tctgcacgga agaacgaacc ttcaggaagg gttaggtaca gcagaacgcc
      961 aatgcacaga acgcccataa ctgcagccca gcgaagtccg cggttctctt cggcggtaac
     1021 cttatgcttc aacagttctt cggaatcctt gtcatgggtt gtatcgccaa gaattttaac
     1081 cgtgaacttt tcggtcacaa aagtggtcac aaccataacg acaatcgttg cgaccgccat
     1141 gaaataccag ttgatcagcg gatgcgtcgg tccggccaca ttcatacctt ttgccgccga
     1201 ttcggtgatt ccggcaagca gcgcgtcggt gcccgcgata aagaagttcg cggtgaaacc
     1261 gccgcttgcc gctgcaaatc cggcgatgac tccaatccac ggatttcttc cgagggcctt
     1321 gaacaccgcg ccgccgattg ccggggtgaa gatgataccc gcgtcggaag caaggttggc
     1381 attgataccc acaacggcga gcacagcagt aacaaggtat gatggagcgc caagaatagt
     1441 ctttctcatc agcgcggaga tcataccgga ctgctcgaca agaccgatgc ccagcgtcat
     1501 gacgacgata agtccaaggg gggcaaaatt cacatacgtc ttgacgaaat ctgccatgaa
     1561 acctcgcatg tacttgaatg aaagcagatt ctcaactgca accgtgactt ctttcggcgc
     1621 ctctcccgcc ttcgccgcca tgtactttac agcgacacct tcgttgccga ggtagtatga
     1681 gagaccaacc actatgagcg aaagaagaac aaagagccag aacggatgag gcagccagtt
     1741 gccgacgatt tcaatccctt tgatgaagct ttcaaaaata ccccttttcc taggttgctg
     1801 attgttttcc atcaatatcc ctccttggta tcaaaatttg caacgatctc ctgtttttct
     1861 acccattagc gtataaccgg gtgcttcaga atacttcctc acgcccctcc tctccttagg
     1921 aacttatctc tccgacaagg caaacgccaa atcggcgaaa agggccgccc cgtaactcag
     1981 gaccctttcg tccacgtcaa aaaactcgtt gtgatgtccc gctgcaatat cggagccaag
     2041 tacaaaatag acggctttcc cgccatgctg ctgtacggcg cgcatcatcc aggaaacatc
     2101 ctcgctgccg ccaagactga cggagggggc aagtttctca atacccggaa cggaaagcgc
     2161 cttttccgcc acaagatcaa cgagctcttt gtcgctgaat gcgtcgacag cctctcccat
     2221 cttggcaacc gacagttcca caccgtacat gactgccgca ccctcgacta tttccatcgc
     2281 tcgaccgtac acatactcgt tcaacgcggt agtcagaccg cgagtttcca ctttcattac
     2341 cgccttcgaa gggacgacat tgcggcctgt ccccgcgtgc agaacaccga cgttgaggcg
     2401 ggttgctcca tctttgtgcg gcgaaatccc ggcaagagcc aaagttgcgg acgccgccgc
     2461 cagaagagcg ttttttccct cgttcggcgc gcctccggca tgcgcggctt tccccttgta
     2521 gacggcgtcg aacttcgttg tgcaaagaaa atcgccgcat ccaggagcaa catttcccgt
     2581 tggaatgccc ataccgatat gcgcgccgag gaagtagtca acatcatcca gaatgccctt
     2641 catcgctacc gcttttcctc cgcggacgcc ctcttcagca ggctggaata ttaatttaaa
     2701 aactcccttg agttcttctt tacgcctcat gagaagttct gcaaaaccaa gaccaatcac
     2761 cgtatgcgca tcatgcccgc acgaatgcat agcgttctcg ttcatggatg caaacccctc
     2821 gcggaaggga cgatgattct cttcggcagc ctccgcaaca tccaccgcat cgatatcgaa
     2881 acgaaaagcg agcgtcgggc cttttttccc ggtatctagc acggcgactg ctcctgtata
     2941 tccgtccatc cgtttaatcc actccgggtc ggctccttgg tgtactgcac gctcaatgta
     3001 tttcgccatc tcatcttccg acgggcgtcc cataacggat tccaaatcga gaatttcctt
     3061 accaacggaa acctgatacc cgaggttctt taaaattcct gcaattttcg ccgtcgtcca
     3121 gaactccgtc cagccagtct cggcatgtgc atgaaactct cttctcaatt gaacgagtcg
     3181 ttgcttcatc gaactgtcca tcgtcgtctt cacgctcctt tacgtccttc cagatattca
     3241 agagcgcagg aaacaagcgt ttccacccct agagaaagcg ctttttcatc cggaagaaac
     3301 atgttgttat gaagaggagg catatcctct ctggctactc cctcaggcct gcatccaagc
     3361 caaagaaaaa gagcggggat ctctttgctg aagaaagcga agtcttctcc tccaagagcc
     3421 ggcttttcta cgtacacaat cttttcttcc ccgacaacct tcttcaaaac agaaaaagcg
     3481 gtctttgcaa gctctggatc attcatgagg ggaggatatc ccttgacgta ctccatgtcg
     3541 caatccccgc ccatacctct tgcgatacca cggacgagcg cttctatacg accgggcatc
     3601 ttctcttgcg tttcagggtt gatcgtccgc actgttccct caaggagcac ttcatcggct
     3661 ataacattgt acctgtttcc gcctttgatt gtacccacgc ttacgactgc cgcatcaaga
     3721 ggactcacgc tgcgcgagac gatcgtctgc agcgcggtga gtatctgcgc cgctatggca
     3781 atggcgtcaa ctccgttttc aggttccgag ccatgggcgc ccttccctcg cacagtcaaa
     3841 aagaggcgat cggaagctcc cataagagcc cctgaacgcg tcccgatcgt gcctgtaaca
     3901 agagagggcc acacgtgcat gccgaaaata gcgtcgacct caggcgcttt gagggctcct
     3961 tgctgtatca tcgcaggcgc ccctccgacc ggattcgcct cctcagcggg ctgaaagata
     4021 cacttgactg taccgggaag ctcatttttc atctcggcaa gtacaagcat cgcacccaaa
     4081 agcattgcgg tgtgcgaatc atgcccgcac gcgtgcatga ttccttttac ttccgacgcc
     4141 cagggaagcc ctgttttctc ctccattggg agggcgtcca tatccgccct aagagcgacg
     4201 cagggacctt ttgcgctccc tcgaagcaca ccgacaacgc catgcccccc gacatcggat
     4261 tgcacttcga ctccagattc ttcaagaacg gacgctacca gcgccgcggt ttcgcgctcc
     4321 tcaccgctca attcaggatg tttgtgaatg cggcggcgga gctccagcat cttctcttcg
     4381 tatttttttg ttaacgaacg tattttctcg ttcactgggg gttcctcctg ttgaattcat
     4441 ttctgctttt ttcacggaag gctgttcaac gtgcgaaaga cagctgacag cggttttcag
     4501 cgcaatcccg gatgttgaac cgctaatgga caattttgaa agacttccgt gataaggtaa
     4561 catagacgcc ggatattttc aacgatgcat gatgcgcgag gcgggtatac agaaaaagac
     4621 cccgtacaaa cctgaaggtt tccacggggt ctttctatta gttgttgctg ttgcgctgaa
     4681 gaacctcttg ccaggtaaca aaggaatgat aatactccat atcgccgtcg tcgaggccgc
     4741 gtgtaatctc cgtaatatgc gtatgcttcg cgcctatctg ttcaagcaca taatcaacgg
     4801 cgatctgcgg cttgctttcc tcgccgcacg tgtaaatatc cagcgccatg tagccgactt
     4861 caggccatgt atgaatagag agatgtgatt cgctgatgac gacgacaccg ctcacgccgt
     4921 cgg
//
LOCUS       DAOPKU010000185         4914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18645_length_4914_cov_5.036839, whole genome shotgun sequence.
ACCESSION   DAOPKU010000185 DAOPKU010000000
VERSION     DAOPKU010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18645_length_4914_cov_5.036839"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PK849_06985"
     CDS             complement(<1..208)
                     /locus_tag="PK849_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65907.1"
                     /translation="MEKEVRSRGAFERGVVVVCLLFAILGGILGYWEMRRASRHVQEE
                     LLRTARLLSESVNPRRLQALSGSAS"
     gene            complement(374..2959)
                     /locus_tag="PK849_06990"
     CDS             complement(374..2959)
                     /locus_tag="PK849_06990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE65908.1"
                     /translation="MNERGRSLSFWFAEGGFLLLLTVILLSGALVGAWRVRETDAFLR
                     EELLRTTRVLAETIDIRDVRRLTGSELDLGSPQYESFKNYFRSVLQMYPRSRFLTLVG
                     RRYDGKVFFFVDSEPPGSEDESPPGEVYDAVDPGFLPAFEEGRAVVVGPVVDQWGAWI
                     SAWVPLVERGSDRVSAALALDISGEEWQRTLEKAKRLPALTSLFLALLLFAGRWMLQQ
                     RTKTGGGAFGRFRYLECLLTLIFGLALTGSVAWMVHERESRSHAQSFSIVAEAHSVKM
                     AGMLYRLRNFVLEGTARFIEALPSLEYESFRRYSQHLSSEPEIDALMWSPRILAEERA
                     EFESTERDEWRRALEIREQNGAGAYVRAEERAEYVPVLFRSPMTGSMFPVGEDLLSHP
                     EVSEALRTAVNTRRPAAAFVRVQYGKEDGSALWVCYPLFRRGFNGALRGVVSALFLPS
                     FMPDEVMGGSGDAGMLSFEALSPEAPPRSLAQWELEGRPEYRFIRPLYAFDGAFAAVL
                     RPGASFLKYHQLRMGLFTSLTGILMTLALVFFVAFTANRRELLEQLVAQRTSELRESE
                     SRHRLAGHIYRSLSEGIVVTDPQGYILDGNAALEHLTGYTLDEIRGRRPGMFSAQRKQ
                     GEDSLHFWERLREHGSWQGETWNRRKDGEAYPVWLTVNAVTDENGEVTHYAGVLTHIG
                     DIKSEQQRLSHLAYHDSLTGLPNRLLLADRLEMALLRSRREHSYVAVLFLDLDGFKEI
                     NDTYGHETGDVLLVSIARRLDEAVREQDTVARLGGDEFILLFEGLGGVEEAHSLAKRV
                     DALFAEPFVAGGRPFLCSASAGLAVHRPGDGATAQEMLAIADMRMYEAKAQRKTSKAL
                     SGSRA"
     gene            complement(2956..>4914)
                     /locus_tag="PK849_06995"
     CDS             complement(2956..>4914)
                     /locus_tag="PK849_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65909.1"
                     /translation="EAHVERAVLVGDLWRLYGAGVHEHVVSTGEGAILPPAWPSVGDL
                     FSVRPLLFFGRTFAVILRPGPGFRALYPPRAGWMSLFAGIMVTAVLTMLAVTVANRRE
                     ELERQVAMRTDELRRSKDELFLMNAALVDTTRKTQEIARRAEAASEAKGRFLANMSHE
                     MRTPLNGFLGMNRLLLDTRLTEEQREYAVAARKSGEALLTLISDALDIATIDEGRYRL
                     VEEDFDLDELLSKLETIFSAAAHEKGLRFTSVRPSVPLRLHGDGRCLYRILRSLLDNA
                     VKFTVRGEVRFSVSALEESDRTVRLRFSVEDTGVGIPSEQQGCVFERFWQGDDSTTKS
                     YQGAGLGLPMAKELAALLGGELEMQSEEGRGTTFSFESLFHKGASPAAPSIQDESVTG
                     RRILLAEDNPVNRKFALAMLRKLGYSVASAENGKEAVDYLSSNECDLVVMDVQMPVMD
                     GVEATRMIRSGASGALDPSIPVVALTAYVSPEDRERCMVAGMNAFLPKPAAPEELDAA
                     IRRLLYGEAGGERAETHVPAEYAGKAAPVFDRAAALKRLMGDEDALAELAEEFIEEMP
                     KDLEKFSALVSEGDAAAAGRCAHSIKGAAAGIGGEALRAAAFEAEKAGKAGDANALLK
                     LEPEVREQYELLEAALRQLISSRGGKNE"
BASE COUNT          932 a         1661 c         1449 g          872 t
ORIGIN      
        1 cggaagcgct gccggaaagg gcttgaagac gccgcgggtt cacgctctcc gaaaggaggc
       61 gggctgtccg gagcagctcc tcctgaacgt gccgcgaagc gcgccgcatc tcccaatacc
      121 cgagaatgcc tccgaggatt gcgaaaagga gacacacgac gacgactccc cgctcaaatg
      181 ctccgcgaga ccgaacctct ttctccatgg ccgggatcct ctctccactg ttcgcagggt
      241 gctttacgcc ggttcccgct tgcgccgcaa atcaaaaatg cagcgggaag gccccctttt
      301 tcgtccgcag aaacaaaaat atcataccac agaacaatct tctccggcaa tacgcactct
      361 tcaggcacat tgctcacgcc cggctgcccg agagggcctt cgaggtcttt cgctgcgcct
      421 ttgcttcgta catccgcata tcggcgatcg caagcatctc ctgcgccgtc gccccgtccc
      481 cgggacggtg aacggccagc cccgcgctgg cgctgcagag gaacggacga cctccggcga
      541 caaacggctc ggcgaacagc gcatccaccc tcttggcgag cgaatgcgcc tcctcgacgc
      601 cgccaagccc ctcgaagagg agaatgaact cgtcgccccc cagtctggcg accgtatcct
      661 gctcccgcac cgcctcgtcg agtctgcggg cgatcgaaac gagcaggacg tcgccggtct
      721 cgtgtccgta cgtgtcgttg atctccttga agccgtcgag gtcgagaaag agcaccgcga
      781 cgtacgagtg ttcgcggcgt gagcggagca gcgccatttc gagacggtcg gccaggagca
      841 gcctgttcgg cagtcccgtg agcgaatcat ggtacgcgag atggctaaga cgctgctgtt
      901 ccgatttaat gtcgccgatg tgggtcagca cccctgcgta gtgggttact tctccgttct
      961 cgtccgtgac cgcgttcacc gtcagccata cggggtaggc ttccccgtct ttacggcggt
     1021 tccaggtctc tccctgccag gagccgtgct cgcgaaggcg ctcccagaag tgaagcgagt
     1081 cctccccctg ctttcgctgc gcggaaaaca tgcccggacg cctgccgcgt atctcgtcga
     1141 gcgtataccc ggtgagatgc tccagcgccg cgtttccgtc gagaatgtac ccttgcgggt
     1201 cggtcacgac gatgccctcc gagagagaac ggtaaatgtg ccccgccagc ctgtgccggg
     1261 actcgctttc acgcagctcg gaagtacgct gcgcgacaag ctgttcgagc agttcgcgcc
     1321 ggttcgccgt gaaggcgacg aagaagacga gcgcaagcgt catcaggatg cccgtgaggg
     1381 aggtaaacag gcccatcctt agttggtggt atttgaggaa ggacgctccg gggcgcagca
     1441 ccgcagcgaa cgcgccgtcg aacgcgtaca ggggacgtat gaagcggtat tccggtctcc
     1501 cttcgagctc ccactgggca agcgagcgcg ggggcgcctc gggagagagc gcctcgaagc
     1561 tcagcatccc ggcgtcgccg ctcccgccca tgacttcatc cggcatgaaa gacggcaaaa
     1621 agagcgccga cacgacgccc ctgagggcgc cgttgaagcc tcgccggaag agggggtagc
     1681 agacccagag cgcggagccg tcttcctttc cgtactggac ccgtacaaaa gcggcggccg
     1741 gtcttctggt attcacggct gtcctgagag cttccgatac ctccgggtgt gaaagaagat
     1801 cctcccccac agggaacatg cttcccgtca tcggggaacg gaagagcacc ggaacgtact
     1861 ccgcccgttc ctccgcgcgg acgtacgccc cggcgccgtt ctgctcgcgt atctccagtg
     1921 cgcgccgcca ttcgtcccgc tcggtgcttt caaactccgc ccgctcttcc gcgaggatcc
     1981 gcggactcca catgagcgcg tcgatctccg gctccgacga cagatgctgc gaatacctcc
     2041 tgaacgactc gtactccagc gaagggagcg cctcgataaa ccgcgcggtt ccctcgagga
     2101 cgaaattgcg gagtctgtag agcattccgg ccatcttgac ggagtgcgct tccgcgacga
     2161 tcgaaaaact ctgcgcgtgg gagcggcttt cacgttcgtg caccatccag gccacgctcc
     2221 ccgtcagggc aagaccgaag atcaatgtca gaaggcactc gagatacctg aatctcccga
     2281 acgctcctcc tcccgttttg gtccgctgct gcagcatcca gcgtccggcg aaaagaagca
     2341 acgcaagaaa gagcgacgtc agcgcgggaa ggcgcttggc tttctccagg gtccgttgcc
     2401 attcctctcc cgagatatcc aacgccaggg cggcgctcac cctgtcggag ccgcgctcaa
     2461 ccaggggaac ccacgccgaa atccatgccc cccactggtc cacgaccggg ccgacgacga
     2521 cggcccttcc ctcctcgaat gcgggcagaa accccgggtc gactgcgtcg tacacttctc
     2581 cggggggcga ttcgtcctcc gaacccggcg gttccgagtc cacaaaaaag aatactttgc
     2641 cgtcgtagcg ccttccgacg agcgtgagga aacggctccg cgggtacatc tgcaagaccg
     2701 agcggaaata gttcttgaaa ctttcatact ggggcgaccc taaatccagt tcgctccccg
     2761 taaggcggcg cacgtcgcgg atgtcgatgg tctccgcaag aacgcgcgta gtgcgcagca
     2821 gctcttcgcg gagaaacgcg tcggtctcgc gcacgcgcca cgcgccgacc aaggcgcccg
     2881 acaaaagaat aacggtgagc agcaagagga atccgccttc ggcgaaccag aaagagagcg
     2941 atcgtccgcg ctcgttcatt cgttctttcc tccccggctg gagatcagtt gacggagcgc
     3001 cgcctcaaga agctcgtact gctcgcgcac ttccggctcc aatttcagga gcgcgttagc
     3061 gtccccggcc tttccggctt tctcggcctc gaacgccgcg gctctgagcg cctctccgcc
     3121 gataccggcg gcggcgccct ttatcgagtg cgcgcacctt cccgcagcgg cggcgtcccc
     3181 ttcggagacc agagcggaaa atttctccaa atccttcggc atctcctcga tgaactcctc
     3241 cgcaagctcc gccagcgcgt cctcgtcgcc cataaggcgt ttcagagcgg ctgcgcgatc
     3301 gaagacgggc gctgctttcc cggcgtattc ggccggcacg tgagtttcgg cgcgctctcc
     3361 tcccgcttct ccgtaaagca gccggcgtat tgcggcatcc agttcttccg gagccgccgg
     3421 cttcggaaga aacgcgttca ttccggcgac catgcagcgc tccctgtcct caggagagac
     3481 ataggcagtg agcgcaacga cgggtatcga cgggtcgagc gcgcccgacg cgccgctccg
     3541 gatcatccgc gtggcctcca ccccgtccat gacgggcatc tgcacgtcca tcaccaccag
     3601 gtcgcattcg ttcgaggaga ggtagtccac ggcttctttg ccgttttcag cggaggcgac
     3661 cgagtacccc agcttgcgaa gcatcgccag cgcaaacttc cggttgacag ggttgtcctc
     3721 cgcaagcagt attcttctgc ctgtcacgga ttcgtcctgt atggaaggcg ccgcagggga
     3781 agcccccttg tggaagagcg actcgaaaga aaacgtcgtt cccctcccct cctcgctttg
     3841 catttcaagc tccccgccga gaagcgcggc aagctccttc gccatgggga ggccaagccc
     3901 cgcgccctgg tacgacttcg tggtcgagtc gtcgccttgc cagaagcgtt cgaagacgca
     3961 cccctgctgc tccgacggga taccgacgcc cgtgtcctcc accgaaaagc gcaggcgaac
     4021 ggtccgatcg ctctcttcaa gggcggagac ggaaaaacgc acctccccgc ggacggtgaa
     4081 cttcaccgcg ttgtccagga ggcttctcag tattctgtaa agacagcgac cgtcgccgtg
     4141 cagccgcagc ggaacagacg gacggacgga ggtgaaacga agtcctttct cgtgcgctgc
     4201 cgcgctgaag atcgtttcca gtttcgaaag cagttcgtcc aagtcgaaat cttcctcgac
     4261 aagacggtag cggccttcgt cgatggtcgc aatatcgagc gcgtcgctta tcagcgtcag
     4321 cagcgcctcg ccggactttc gagccgcaac ggcgtactcc cgctgctcct ccgtcagacg
     4381 ggtgtcgagc agcagccggt tcatcccgag gaagccgttc aacggcgtcc gcatctcgtg
     4441 actcatgttc gcgaggaagc gccccttcgc ctcgctggcc gcctcggcgc ggcgcgctat
     4501 ctcctgcgtc ttgcgcgtcg tatccacgag ggccgcgttc atcaggaaga gctcgtcctt
     4561 gcttcggcgc agctcatccg ttctcatcgc gacctggcgc tcaagctcct ccctgcggtt
     4621 cgcgactgta accgcgagca tggtcagcac agcggtcacc atgatgccgg cgaaaaggct
     4681 catccaaccg gcacggggcg gatagagcgc acgaaagccg ggtccgggac gcagaatgac
     4741 ggcgaacgtc ctgccgaaga agagaagggg gcgcaccgag aagagatccc cgacggacgg
     4801 ccatgcaggc ggcaaaatgg cgccttcccc cgtggagacg acgtgttcgt gaactcccgc
     4861 gccatacagg cgccacaaat cgccgacgag cacggcgcgc tccacgtgcg cctc
//
LOCUS       DAOPKU010000186         4903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18704_length_4903_cov_3.871081, whole genome shotgun sequence.
ACCESSION   DAOPKU010000186 DAOPKU010000000
VERSION     DAOPKU010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18704_length_4903_cov_3.871081"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="PK849_07000"
     CDS             complement(<1..1425)
                     /locus_tag="PK849_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPE65910.1"
                     /translation="MRKAGRILLLLLIAAIFIALSATAFMLAASTESASRVRLDWAHM
                     ANISAERVLSPADRSSPRAAMESFMNHMKEGYERLLKAKELVSSAPGLLHQPPEALEE
                     AGRALYNFERAMHVLDLSQIPQVVRLQNARENILLLKEILDRVGIPRYDQIPGAQEVE
                     EQGLVRWTIPKTEIRLHRIESGIDEGRWLVTPASVAELGRSYRRVRHLPYLPGATENA
                     YEYYTASPGAFFPPKWAYFIPAWLTHRIYGQALWQWSILIVSSILFILLLLAVRKLSF
                     KISEGRSPVFKQLLKLTAPFSLALGAIAMVWVIEEQINITGTLYLLLINVLTAVEWLG
                     WTWVIVRAGELLAELIILSPRIESGSVDASLVRTLSRLASIAAAIGILMFGASELGVP
                     LMTLITGFGVAGLAVSLAARPTLENIIGGIILFTDRSVKVGEFCRFGDKAGTVLHIGL
                     RSTKIEALDQTVISIPNADFSQMQI"
     assembly_gap    1589..1688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1689..2444
                     /locus_tag="PK849_07005"
     CDS             <1689..2444
                     /locus_tag="PK849_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPE65911.1"
                     /translation="MMRMKKEEYRWFLKDTVRTTVDFSQTQQNLGYPPPPVQKPRPEG
                     SHIIPLPGPEEAGALCDISVAEAIRRRSSHRKYSVKPLAIEELSFLLWATQGVRGDRD
                     PVRSYRTVPSAGCRHPFETYLAVFRVESLQKGMYRYLPFDHALLPLGCPSDLESRMSR
                     AAHNQPFAGKSAVTFIWTALPERTEWRYSEASVKVIALDAGHVCQNLYLACEAADAGT
                     CAIAAYDQELADSLIGVDGENEFVLYMAPVGKL"
     gene            2536..3999
                     /locus_tag="PK849_07010"
     CDS             2536..3999
                     /locus_tag="PK849_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPE65912.1"
                     /translation="MLLKKNGVLLLVLAVCAAAVLGAGTVFAEQSETVVFEVTADTPV
                     VQTGEARKVVVRALVRPYTRRETVRAPLAVALVLDKSGSMASDGKMDNAKRGALEALK
                     MLDSRDVATIIVYDDNASVLVRARSVGNGAKEPAFLRAISRVRSGGMTALHDGVVLGA
                     EQLEPFIDEGFIPRIVLLSDGIANVGPSSTEELARLGRGLARREMTITTIGLGLDYHE
                     DLMTALAAESGGNAYFARNARMLPEIFTRDMEDAVTLTARRVRISLRALGGARPARVI
                     GRPGNLTGDLLEASIDNLYSGEKYALFEMELPEGEEGAQLEAAEVVLEYIDPVTGDTV
                     RKSAPLTFSFTADGAEVEKRQRTDIVSQAALARNAEIREEAVRLADEGKAQEAAQMLG
                     DRTKQLRMLAPSVGTAAPQMDKESEYFEDLAESLLSTGGLSNVERKEVLNDAYMQKTQ
                     QAPVEVVSGDLPGDKPEDTPEDESEEEEKEGEGNASK"
     gene            4096..>4903
                     /locus_tag="PK849_07015"
     CDS             4096..>4903
                     /locus_tag="PK849_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65913.1"
                     /translation="MEKMRDVLKRLTTPFSLTLLFFLAVLLPSVVLSMLALRAADREA
                     LHIERNLESALLSEVNLAAHRVADLLGSIATELMGEASEIPSNPAFLEQWRGGGALAV
                     SPFLLVHGRLSLPDSPPEAVETLLLSFGAFLEGRAELPVYDHIAGVYRRELNDLDEVA
                     PSTLSRREDVAGATGLRTPAERNRPGGAIPESVPYSAEDSIAFQAAEAPADFSSHDGA
                     FLMDEEADAGYEPSPKEGAKMQKSLRAASPAPASPPAPLALFPPLPKKLAQ"
BASE COUNT         1000 a         1299 c         1508 g          996 t
ORIGIN      
        1 gatctgcatc tgcgagaagt cggcgttcgg aatggagatc accgtctgat cgagcgcttc
       61 gatcttcgtg ctccgcaagc cgatgtgaag tacggttccc gccttgtctc cgaaacggca
      121 gaactcgccg accttgacgg accggtcggt gaagaggatg atgcctccga tgatgttctc
      181 aagagtcgga cgggcggcaa gagagacggc caaaccggcg acaccgaatc cggtgatgag
      241 ggtcatcagc gggacgccga gctcggaggc tccgaacatc agaataccga tggcagcggc
      301 gatgcttgcg agacggctca gcgtccgcac caggctggcg tcgacgctcc cggactcgat
      361 tctgggcgag agaataatga gctccgcgag cagctcgccc gcccggacga tcacccacgt
      421 ccagcctaac cactcaacag ctgtaaggac gttgatcagc aaaagataga gcgttccggt
      481 gatgttaatc tgttcttcga tgacccagac cattgcgatt gctccaaggg cgagcgaaaa
      541 cggggcagtc agcttcagga gttgtttgaa gacaggcgac ctgccctccg atattttaaa
      601 actcagtttc cggacggcca gaagcagaag gatgaacaga attgacgaga caatgagaat
      661 gctccactgc cagagggctt gaccgtaaat acgatgcgtc agccacgccg gaatgaagta
      721 ggcccacttg gggggaaaga aggctcccgg agatgctgta tagtattcat aagcgttttc
      781 ggttgctccc ggtaggtatg gcagatgccg tacccttcgg taagaacgtc ccagctcggc
      841 aacggaggcc ggcgtgacga gccagcgccc ctcgtcgatc ccggattcta tccggtgcag
      901 acgtatttcg gtttttggaa tggtccagcg gacgagtccc tgttcttcga cttcctgggc
      961 tccgggtatc tgatcgtacc gggggatgcc cacccggtcg agaatctcct tcaagagaag
     1021 aatgttttcc ctggcgttct gaagacggac gacctggggg atttgtgaga ggtcgaggac
     1081 gtgcatggcg cgttcaaagt tatacagggc gcgtcccgct tcttccagcg cctcgggcgg
     1141 ttgatggagg agtcccggag cggaggagac gagctccttc gccttgagca gtcgttcgta
     1201 gccttctttc atatggttca tgaaggattc cattgccgcg cgaggagacg atctgtctgc
     1261 gggagagagc acgcgctccg cgctgatgtt cgccatatgg gcccagtcga gtcgaaccct
     1321 ggaggcgctc tccgtcgagg cggcaagcat gaaggccgtt gctgaaagag cgatgaaaat
     1381 cgccgcgatg agaaggagca gaagaatccg gccggctttt ctcatgagtt gaaccacttc
     1441 ctttcgtgag ctgcgtatgc ctataatgtg gacgaaatga aaaaacgtcg ttcgcacaag
     1501 aaaggctgaa acggcgggta ttctaatatg tgtgccgtgt gcacaaattg cagagttttt
     1561 gctgtgtgca atacatttta tcagaaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnat gatgagaatg aaaaaagaag agtatcgctg gtttttgaag gatacagtac
     1741 gcacgaccgt cgatttttcg cagacgcagc agaatcttgg atatccgccg cctccagtgc
     1801 aaaaaccgcg cccggagggc agccatatca ttccgctgcc cggaccggag gaggcgggag
     1861 ctctctgtga tatttccgta gcggaggcca tccgtcgccg cagctcccat agaaaatatt
     1921 cggtgaagcc tctggccatt gaagagttga gctttttgct gtgggcgacg cagggagtca
     1981 gaggcgaccg cgatcccgtc cgctcttacc ggaccgtacc ctccgcagga tgccgtcatc
     2041 cgttcgagac ctatcttgct gtttttcggg tggaatcgct ccagaaggga atgtacaggt
     2101 accttccttt tgaccacgcg ctgcttcccc tcggatgtcc gtccgacctt gaatcgagga
     2161 tgagcagggc tgcgcacaat cagccgttcg ccgggaaaag cgcagtcacg ttcatctgga
     2221 cggcgcttcc ggagcgtacc gaatggcgct attccgaggc ctccgtgaag gtgatcgcgc
     2281 tcgacgcggg gcatgtctgc cagaacctct atcttgcatg cgaagccgca gacgccggaa
     2341 cgtgcgccat tgccgcctac gaccaggaac ttgccgacag tctcatcggt gttgacggag
     2401 agaacgagtt tgtcctctac atggcgccgg tagggaaact ctgatgattt ttacattttt
     2461 gtgacatccg cttgacgggg cgatgacatt ctgccggttt tttcgctgta tccttcggat
     2521 caggaggtgg tgaagatgtt gttgaaaaag aatggagtcc tgctgctggt tttggcagtg
     2581 tgcgccgcgg cggttctcgg cgctggaact gtttttgcgg aacagtctga gacggtggtc
     2641 tttgaggtta ccgcggatac ccccgtcgtg cagaccgggg aggctcggaa ggttgttgtc
     2701 agggcgcttg ttcgtccata taccagaaga gagacggttc gggccccgct tgctgtcgct
     2761 ctggtgctcg acaaatcagg atcgatggcg tccgacggga agatggacaa tgcgaaacgt
     2821 ggagctctcg aagccctcaa gatgcttgac agccgggatg tggcgacaat aatcgtgtac
     2881 gacgacaacg catccgttct ggtacgggct cgctccgtcg gaaacggtgc gaaagaaccg
     2941 gcttttctca gggcgatttc ccgggttcgc tccggaggaa tgacagcgct gcacgacggc
     3001 gtcgtccttg gtgcggagca gctcgaaccc ttcatcgacg aggggtttat cccccgtatc
     3061 gtgctgcttt ccgacggaat cgccaacgta ggtccctcgt cgaccgagga gctggcgcgt
     3121 ctcggcaggg gattggcccg cagggagatg acgattacga cgatagggct cgggcttgat
     3181 taccacgagg atcttatgac ggcgctcgct gcggagagcg gggggaacgc ctattttgcc
     3241 cgcaatgcga gaatgctgcc cgagatcttc acccgggata tggaggatgc ggtgacgctg
     3301 acggcccggc gggtgcgcat ttcgctccgc gctctcggcg gcgcgcgtcc ggcgcgtgtc
     3361 attggccggc cgggaaacct caccggcgat cttttggagg cctccatcga taacctctac
     3421 agcggagaaa aatacgcgct ctttgaaatg gagctccctg aaggggaaga gggcgcccag
     3481 cttgaagcgg cagaagttgt ccttgagtac atcgatcccg tgaccgggga caccgtgcgg
     3541 aagagcgctc cattgacctt ctcctttacc gctgacggcg ccgaggtgga gaagcgccag
     3601 aggacggata tcgtctcgca ggctgctctg gcccggaacg cggagatcag agaggaggcc
     3661 gttcgcctgg ccgacgaggg gaaagcgcag gaggcggcgc agatgctggg agatcggacg
     3721 aaacagcttc ggatgcttgc cccttccgtc gggacggcag ctccgcagat ggacaaggaa
     3781 tcggagtatt tcgaggatct ggcggagagt ctgctctcga caggcgggct ttcgaacgtg
     3841 gagcggaaag aagttcttaa cgacgcctat atgcagaaga cccagcaggc tccggtagag
     3901 gtcgtgagcg gcgatcttcc cggggacaaa ccggaggata cgccggagga tgaatcagag
     3961 gaagaagaaa aagagggaga gggaaacgcc tcgaaataag aggcgatcct tccggagaac
     4021 aggagatagt atgaagaaaa aggccgggga cgttttcgtt cctggccttc acgtaaagga
     4081 aaggaggacg gcgcaatgga gaagatgaga gacgtgctca aaaggttgac taccccgttt
     4141 tctctgacgc tgctcttttt cctcgccgta ctgctcccga gcgtcgtctt gagcatgctc
     4201 gcgcttcgcg ctgcggacag ggaggcgttg cacatcgaac gcaacctgga gagcgccctg
     4261 ctctcggagg ttaatctcgc cgctcaccgg gtggccgact tgcttgggtc gattgcaacg
     4321 gaactgatgg gggaggcgtc ggagatccct tccaatcccg cctttctgga gcagtggaga
     4381 gggggaggag cgcttgccgt ctctcccttt ttgctcgtgc acggacgtct ctctcttcct
     4441 gattcgcccc ctgaggcagt cgaaacgctt ctcctttctt tcggggcgtt tcttgaagga
     4501 cgagccgagc tgccggttta tgatcatatc gccggtgtat accgcaggga attgaacgat
     4561 ctcgacgagg tcgctccctc caccctttcg cgcagggaag acgtcgcggg agcgacgggc
     4621 ttgcggactc ccgcggaaag gaatcgcccc ggcggtgcga ttcctgaatc cgttccgtac
     4681 tcggcggagg attccattgc attccaggct gcggaggcgc ctgcggattt ttcttctcat
     4741 gacggagcgt ttctcatgga cgaggaagcg gacgccggtt atgaaccctc tccgaaggag
     4801 ggggcgaaga tgcagaaatc acttcgcgcg gcttctcccg cacccgcctc tcctcccgcc
     4861 cccctcgcgc tcttcccgcc gcttccgaag aaacttgcac agc
//
LOCUS       DAOPKU010000187         4886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18810_length_4886_cov_4.926930, whole genome shotgun sequence.
ACCESSION   DAOPKU010000187 DAOPKU010000000
VERSION     DAOPKU010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18810_length_4886_cov_4.926930"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PK849_07020"
     CDS             complement(<1..483)
                     /locus_tag="PK849_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65914.1"
                     /translation="MKRVVAAFPGTCGELFQGTLDGVQCLVSCPVDRTARLELILEEG
                     EGISVPPEMSKTRRALELALARRPLAGALRVRRLEALPEGKGYASSTADILAALYGLA
                     NLSGTPLTPEDASRIALAVEPTDSIAWSGLALLDHREGRVMQFLGPPQTRREAAGDRG
                     T"
     gene            complement(480..1964)
                     /locus_tag="PK849_07025"
     CDS             complement(480..1964)
                     /locus_tag="PK849_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HPE65915.1"
                     /translation="MIQGTSSSVGKSVLVAALCRIFARRGLKVAPFKAQNMALNSFVT
                     SAGEEMGRAQAVQAAAAGRAPEVEMNPVLLKPEGNSRSQVVLMGRPWKTLSAGEYYAC
                     REVLWGSVVASFEKLASENDLVLVEGAGSPAEINLKKHEIVNMRVARTFGCPVLLAGD
                     IDRGGVFASLVGTLALLDEEERALVKGFLINKFRGDVKLLEPGLDMLSGLTEGRPVLG
                     VVPWLKNLAVAQEDSVWLEERTAGDSSGGVDIAVIKLPRISNYDDFDPLALEEGVSLR
                     FVERPGDLGHPAVVILPGSKTTLKDLAWLRSSGFDGLIAALALAGGSVVGVCGGYQML
                     GRRISDPLGVEGPASEESGLGLLPVETVFEPAKETTLSRGRTIPSSESPASGFLAQPF
                     DVEGYEIHMGRSVLPDGARPLLRFEDGRVDGAVSAGGHVWGTYLHGIFDNGPFRTAWL
                     RSLGWKGKESPLSFAQVRNQAFDRLADEVEAALNMELLERIIGL"
     gene            2163..2642
                     /locus_tag="PK849_07030"
     CDS             2163..2642
                     /locus_tag="PK849_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65916.1"
                     /translation="MNKRSVLLRFLAFLCVVSLLAPAAFAAQQIPPEVKSYVIKFFNE
                     YGNATKTMADRVQKTDDPEQIAGALDKYVDTVAPLMKGMDDLQKKYKDFFDAMEEEDR
                     EDSGDADIDKANAEFEKTQQGMMDAMQKIASHMEHPKVEAAMERLEKIMSDMDETAE"
     gene            complement(2726..3424)
                     /locus_tag="PK849_07035"
     CDS             complement(2726..3424)
                     /locus_tag="PK849_07035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPE65917.1"
                     /translation="MQLSADAVSVLLIVAVSALLVGTGLRKQPGIGAAASVLLIAGAL
                     WFRGESPSAIGFGPQEDWGGTILLGFAYAALLQLLAVTLVGPLSEKLTGTEPDHSVFD
                     GVRGNWRTLLLWLALVWTLVAFLEEGIFRGFLMTEIVKVAGTGTLALCFNVLFTSVVF
                     GLAHGYQNRSGMVSAGMVGLFLGALFIFEGFNLWLVIFVHGFVDTIGIVLIAAGADRS
                     IDGFIRRLWRMNPH"
     gene            complement(3464..3853)
                     /locus_tag="PK849_07040"
     CDS             complement(3464..3853)
                     /locus_tag="PK849_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPE65918.1"
                     /translation="MRLHHIALWSKDIERLSAFYRKYFGACAGETYVNRGKGFSSIFL
                     RFEEGCSIELMHMASIPGNANDPEAQYIGLIHMAISVDGEGEVDAKTEELRRDGYTIV
                     SEPRRTGDGYYESCVFDPDGNRIEITV"
     gene            complement(3850..4500)
                     /locus_tag="PK849_07045"
     CDS             complement(3850..4500)
                     /locus_tag="PK849_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPE65919.1"
                     /translation="MRCCIDHIAVTAPSLESGAEFIREALGVSPQPGGEHPRMGTHNM
                     LLRLGETVYLEVIAPNPNAERPGRPRWFGLDTLRPDSAPRLGAWVARTEDIYTASTAA
                     SERLGEVEAMTRGSLEWHITISADGSLALGGLAPSLIQWKAGEPHPAGRLEDRGLSLV
                     RLELFHPEPERVSRLLRSLEFEGPVSVEEARPGEAPYLVAQIRTPLGLRTLSGRHA"
     gene            complement(4545..>4886)
                     /locus_tag="PK849_07050"
     CDS             complement(4545..>4886)
                     /locus_tag="PK849_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPE65920.1"
                     /translation="TEGPKTSSYSIAPVENADVMRETLAAALGQSGRVRKRRTLFLVG
                     RTRVHIDRVEDLGNFLELEVVLEEGEPEADGVREAEELLAALAIPQSALVDRAYVDLL
                     EARGKGEPSRP"
BASE COUNT          987 a         1701 c         1414 g          784 t
ORIGIN      
        1 agtgcctcgg tcccccgccg cctctcgccg tgtttgtggt ggaccgagga actgcatgac
       61 gcgcccctcc cgatggtcga gcagcgccaa gccggaccac gcgatactgt ccgtcggttc
      121 gacggcaagc gcgattcgcg acgcatcctc cggcgtcagc ggcgtccccg aaaggttcgc
      181 caagccgtag agcgcagcaa gaatatccgc cgtgctgctg gcatacccct ttccctccgg
      241 aagcgcctca aggcggcgaa cccgcagcgc gccagcgaga gggcgacggg caagcgccag
      301 ttcgagcgct ctccgcgtct tgctcatctc gggcggaacc gaaatgcctt ccccctcttc
      361 aagaatcagc tccagccggg ccgtccggtc gacgggacag gagacaaggc actgcacacc
      421 gtcaagcgtc ccctgaaaaa gctccccgca cgttccgggg aaggccgcga cgacgcgctt
      481 catagcccga tgatccgctc cagcagctcc atgttgagcg cggcctccac ctcgtccgcg
      541 agacggtcga aagcctggtt ccgcacttgg gcgaaggaga gcggactttc tttccccttc
      601 cagccgagcg agcgcagcca tgcggtccgg aacgggccgt tgtcgaagat gccgtgcaga
      661 taggttcccc agacgtgtcc gcctgcggag accgcgccgt cgacgcggcc gtcctcgaac
      721 cggagcagcg ggcgagcccc gtcaggcagc acgctccgtc ccatgtggat ctcgtacccc
      781 tccacgtcga acggctgcgc aagaaaaccg gaggcggggc tctccgaaga cggtatcgtt
      841 cgccctcttg aaagcgtcgt ctccttcgcc ggttcgaaga ccgtctcgac gggcagcaga
      901 ccgagcccgg actcctccga cgcggggccc tccacgccga gcgggtcgga aatgcgccgt
      961 ccgagcatct ggtagccgcc gcagacgccg acgacggaac ctccggcgag cgcgagggcg
     1021 gcgatcagtc cgtcgaagcc ggaactgcgc agccaggcca ggtccttcag cgtcgtcttg
     1081 ctccccggaa ggataacgac agccggatgg cccaagtccc ccggacgctc cacgaaacgc
     1141 agcgagaccc cctcttcaag cgcgagcggg tcgaagtcgt cgtagttgga gatgcggggg
     1201 agcttgatca cggcgatgtc cacgccgccg gaggaatcgc ccgccgtgcg ctcttcaagc
     1261 cacacggagt cctcctgcgc cacggcgagg ttcttcagcc acggaacgac gccgagcacc
     1321 ggacgcccct ccgtgagacc ggaaagcatg tcgagtcccg gttccagcag cttcacatcc
     1381 ccccggaact tgttgatcag gaaccccttc accagcgcgc gctcctcctc gtcgagcagc
     1441 gcgagcgtcc ccacaagcga ggcgaagacg ccgccccggt cgatgtcccc cgcgaggagg
     1501 accgggcagc cgaacgtgcg cgccacgcgc atgttcacga tctcgtgctt cttcaggtta
     1561 atctccgccg ggctccccgc tccttcgacg aggacgaggt cgttctccga ggccagcttc
     1621 tcgaacgacg cgaccacaga accccacaaa acctccctgc aggcgtagta ctcgcccgcc
     1681 gaaagcgtct tccacggcct ccccatcagc accacctgcg agcgggaatt cccctccggc
     1741 ttcagcagca cggggttcat ctccacctcc ggagcgcgcc ccgcggcggc ggcctgcacc
     1801 gcctgcgcac gccccatctc ctctcccgcc gaggtgacga aggagttcag cgccatgttc
     1861 tgcgccttga acggggccac tttcagcccc ctccgcgcga atatgcggca gagcgccgcc
     1921 acgaggacgc tcttccccac ggaagaggac gttccctgaa tcatcagtct ggacgcacga
     1981 atagcaattc ctccgatccg gtgaattcct ggacaatcct acccagtagc gggcgttttg
     2041 gcaagccgag gagcggaaca agcggcgtgc gaaaacctaa aaaagcacgc agtacgattg
     2101 ctttcctctc gcgagattgt gatacaatga gcgccatctt accgaaagaa agaggtgtac
     2161 ccatgaataa acgctccgtt cttctccgtt tcctggcatt tctctgtgtc gtttcgctcc
     2221 tcgccccggc ggcgttcgct gcgcagcaga ttccgcccga ggtcaaaagt tacgtgatca
     2281 aattcttcaa cgagtacggg aacgccacaa aaaccatggc ggacagggta caaaaaaccg
     2341 acgaccccga gcagatcgca ggcgcgctcg acaagtacgt cgacacggtc gcgccgctga
     2401 tgaagggcat ggacgacctg cagaagaagt acaaggactt cttcgacgca atggaagagg
     2461 aggaccggga agacagcgga gacgccgata tcgacaaggc caacgccgag ttcgaaaaga
     2521 cgcagcaagg aatgatggac gccatgcaga agatcgcctc gcacatggaa cacccgaagg
     2581 tggaggccgc tatggagcga ctcgaaaaga tcatgtccga catggacgag accgcggaat
     2641 aagcgtttcc taaaaagctc ctcaccgaaa gaataaatga aacggcccgg ccgctcttca
     2701 cgccgggccg tttcattgaa atcgctcagt gcggattcat cctccagagc ctccggatga
     2761 atccgtcgat gctccggtcc gctcccgccg cgatcaggac gataccgatc gtgtcgacga
     2821 agccgtgcac gaagatcacc agccacaggt tgaacccctc gaagatgaag agcgctccca
     2881 gaaagagccc caccatcccc gcgctcacca tgccgctgcg gttctggtag ccgtgcgcca
     2941 ggccgaagac gacggaggtg aagagcacgt tgaagcagag tgcgagcgtt cccgttccgg
     3001 caaccttcac gatctcggtc atcaggaacc cccggaagat tccctcctcc agaaaggcaa
     3061 ccagcgtcca caccagcgcg agccacaaaa gcagtgtcct ccagttcccc ctgaccccgt
     3121 cgaagacgct gtgatcaggc tccgttccgg tgagcttttc gctcagcggc ccgacgagcg
     3181 tcacggcaag gagttggagc agcgcggcgt acgcgaaccc gagaagaatc gttccacccc
     3241 agtcctcctg cggaccgaag ccaatggccg agggagattc gccccgaaac cagagcgcgc
     3301 ccgctatcag cagaaccgac gctgcggccc cgatccccgg ctgttttctc aggccggtac
     3361 cgacaagaag cgccgagacc gcaacgatca ggagcacgga gacggcgtca gcggaaagct
     3421 gcatgaggac gctctttcac cgcttcacag ctcgccgcgg acatcatacg gtgatctcta
     3481 tcctgttgcc gtcggggtcg aagacgcagc tctcgtaata cccgtctccc gtcctgcgag
     3541 gctccgaaac aatcgtgtat ccgtcgcggc ggagttcctc cgtcttcgcg tccacttccc
     3601 cctcgccgtc gacggagatc gccatgtgga tcagtccgat gtattgcgct tcggggtcgt
     3661 tcgcgttccc cgggatggac gccatgtgca tcagctcgat gctgcaccct tcctcgaaac
     3721 gcaggaatat cgaggaaaac cctttccccc tattgacgta cgtttcccca gcgcacgcgc
     3781 cgaagtactt ccggtaaaaa gcggagagac gctctatgtc cttggaccag agcgcgatat
     3841 gatggagcct catgcgtgcc gccccgaaag cgtccgcagt cccagaggag tccgtatctg
     3901 cgcgaccaga tacggcgcct cgccaggacg cgcctcctcc accgaaacag gcccttcgaa
     3961 ttcgagcgaa cgcaacagac gggagacacg ctccggttcc gggtgaaaga gctccagccg
     4021 gacgagcgac aggccgcgat cctcaagcct acccgcagga tgcggctctc ccgctttcca
     4081 ctggatcagc gaaggggcga gccccccgag cgcgagcgaa ccgtcggcgg agatggtgat
     4141 atgccattcg agtgaaccgc gcgtcatggc ctccacttcg ccgagacgtt cggacgccgc
     4201 cgttgaagcc gtgtaaatat cctccgtgcg cgccacccac gcgcccaacc tcggcgcgga
     4261 gtccggacga agcgtatcca ggccgaacca gcgcggccgc ccgggccgct ccgcattcgg
     4321 attgggcgcg atcacttcga gatacacggt ctcccccagg cgcaggagca tattgtgcgt
     4381 ccccatccgg gggtgctccc ctcccggctg cggagagacc ccaagcgcct cgcggatgaa
     4441 ttcggcccca ctctcaagcg acggggcggt tacagctata tgatcgatgc aacagcgcat
     4501 cttctcaacc tccttcaatg aatggaatcc tgccgatgcg gcgcttacgg ccgagaaggt
     4561 tcacccttcc cgcgggcctc cagcagatcg acgtacgcac ggtcgacgag ggccgactgc
     4621 ggaatcgcca acgccgccag aagctcttcc gcttcgcgga ctccgtcggc ctccggctcc
     4681 ccctcttcga ggaccacctc cagctcaagg aagttgccca aatcctccac gcggtcgata
     4741 tgcacgcgcg tccgcccgac caggaagagc gtccgtcgtt tgcgtacccg ccccgactgc
     4801 cccaaggctg ccgcgagcgt ctcccgcatc acgtcggcat tctccacggg agcgatgctg
     4861 taagacgacg tcttcggccc ctccgt
//
LOCUS       DAOPKU010000188         4871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18893_length_4871_cov_4.608389, whole genome shotgun sequence.
ACCESSION   DAOPKU010000188 DAOPKU010000000
VERSION     DAOPKU010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18893_length_4871_cov_4.608389"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2595
                     /locus_tag="PK849_07055"
     CDS             <1..2595
                     /locus_tag="PK849_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65921.1"
                     /translation="CALAAGLLLRLPFAGGAEETAGPTAMEEGEAVFQLSSGDVSAAS
                     VLSQSPAVEEAAGPLQTAEKTSEEGIPDAPSAETAGNELGATSADVPVSAGSEQLARK
                     LFEIEVASRVAEEELGKLPSLVQSERTKIANELNQWANTDLRRIIDQHYLEVEKALAE
                     TTLPEDHSSVGIWWKQVKAGVFLNEEIEEVVALFVKRAGPRLSTVHEEFQRTLLESLE
                     QRSQTLLRRSMEEIRTPFVATVKDRLPLYNAIPVPNTERAALSIALSGEQSDRGRLSS
                     GMAVIGGVVLTVLGRKLVQKMMHLAVVKIGGKVLSRMIPIIGVGLLLYEGIQMGQAKE
                     AFEEELRKSFFTEYTADVTVESVWNASPDGTLPSMKQEMERNINTLLSDWERICRSEA
                     VSMIHSAQVLASSEHVRNYVSGELEKGADFQLLLQKINALWDAFGQSLALEPVEFFES
                     ILIDSPDRNDLRLLSRDPGSRFIELYKEWGAEFLKAVNKIGVENYVSTEWKTSDVNWS
                     VLNRSLDFLPDISGNRNAARGLLFLIQEGVPLDGFSPDLMAKIAVKKDVFLKVWQALT
                     PDSRKLAVIFESGRPLVILEPSLIAFPEACSLFLRDYGVEFWTSWSSDDIFDLLRISE
                     IRGKNSGKLRAASVLPEERADLLEIYRRSGERGIILWDIHTKEPTGEIGRKLARQSIA
                     CMAEGYPFEDMKDPEFVRFAAATLNFPAGRFFYDTLKGVGSLVRYLLLGGVLLLFGGV
                     FFGLFKRFRGAFFSPPSRYTIKEGTEKTERKALKDVFADDERADGGGADETASFTVEP
                     EMIEDPEISPAETDDATGKEEDLQEEDAVSADEELQRGQSDEESAGTAGKSTRQRRRK
                     KRMKKENE"
     gene            2588..2926
                     /locus_tag="PK849_07060"
     CDS             2588..2926
                     /locus_tag="PK849_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HPE65922.1"
                     /translation="MSKKGIPVLTVDVIPGKKCEYLGCVSASCCLSKSLVQDVASNVK
                     NWTVGGELTRYTDMIDDAVNLVTERLGVLAQQMGANAIIGFRLSTTSVSTGAAELIAY
                     GTAVRFPDLN"
     gene            complement(2923..4089)
                     /locus_tag="PK849_07065"
     CDS             complement(2923..4089)
                     /locus_tag="PK849_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE65923.1"
                     /translation="MDRLIVSLMIINFAIHSYSNTFFFLAPYLSTQGYSPASAGVLVS
                     VFYAATTAVRPVGSWITERFGIRGSMIASSLLCLACAAVLHIYQGSFSFFVGLRIVMG
                     IGFSVFMVALTTYQALAVEEAKRGLAFALVAIGSIVPMFTIVPLTDFLIRNNLGRIYL
                     LLPVAASALALMLAIRLNTKQTTGFEETNSTMKWGSYRDLFRETPAWRMLCSCFFFGL
                     CDSSIVFLGSMLLEKGLVPSWFVFAMAGGALFVRTAGRNLFNRYPRTVFAGPSFLLMA
                     ACLIWLSFVDSPAGMLLSGFLYGIGVGYGYPAHLALTADLAPARLRAKASALVHFSMD
                     LSWFLLPLYMGFGSTLLGTLLAFRLFALFCLITAVAVTVMWLPGKGRAGGGAPA"
     gene            complement(4285..4455)
                     /locus_tag="PK849_07070"
     CDS             complement(4285..4455)
                     /locus_tag="PK849_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE65924.1"
                     /translation="MAKAVVDKDACVGCETCVGTCPVEAISMVDGKALVDESCIECGS
                     CVSVCPVSAITQ"
     gene            complement(4575..>4871)
                     /locus_tag="PK849_07075"
     CDS             complement(4575..>4871)
                     /locus_tag="PK849_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HPE65925.1"
                     /translation="VLTCYSTQSRNFRLGLALGRGLSLDEAKLEVGQVAEGAFTVRAV
                     AESALALSVELPVSQGVHRLLYEGSSPQDELERLIARDPKAEYPPAMLWGSGRG"
BASE COUNT         1094 a         1345 c         1527 g          905 t
ORIGIN      
        1 tgtgcgcttg ctgcggggct gctccttcgc ctcccgttcg cgggtggagc ggaggaaacc
       61 gcgggaccaa cggcaatgga agagggggaa gctgtctttc agttgtcttc gggcgacgtg
      121 tccgcagcgt ccgttctttc acagtctccg gctgtcgaag aggcggcggg tccgctccag
      181 acagctgaga agacctccga agagggaatt ccggatgctc cgtccgcaga gactgccggg
      241 aacgagctgg gagcaacctc cgcggacgtc cccgtgtccg cagggtcgga gcagcttgcc
      301 aggaagctct tcgagattga ggttgccagc cgcgtcgctg aagaagagct tggaaagctc
      361 ccgagcctcg tgcagtccga acggacgaag attgcgaacg aactgaacca gtgggcaaac
      421 acggatcttc gcaggatcat cgatcagcac tacctggagg tggagaaggc gctcgcggag
      481 acgacgcttc ccgaggacca cagctctgtt ggtatctggt ggaagcaggt gaaggcgggc
      541 gttttcctga acgaggaaat cgaggaggtc gtcgccctct tcgtgaaacg cgcaggcccg
      601 aggctctcca ccgtgcacga ggagtttcag cgaacgctgc tcgaaagttt ggagcagcgc
      661 agccagacgc ttctgcgccg ttctatggaa gagattcgca caccattcgt cgctacggtc
      721 aaagacagac ttcctcttta caatgcaatc ccggtgccca atacggagcg cgccgcattg
      781 tcgatcgccc tttcagggga acagagcgac aggggacgtc tctcgtcagg tatggctgtg
      841 atcggcggcg ttgtgctcac cgttcttggg cggaagctgg tccagaaaat gatgcacctg
      901 gcggtggtca agatcggagg aaaagtgctc tcgcggatga tccctatcat cggggtcggc
      961 ttgcttctgt acgagggcat ccagatggga caggcgaaag aagcgttcga agaggagctc
     1021 aggaagagct tttttacgga gtacactgcc gacgtcaccg tggagtccgt ctggaacgcg
     1081 agtcccgatg gaacgctccc ctctatgaaa caggagatgg aacggaatat caacacgctg
     1141 ctgagcgact gggagcggat ctgccgttcc gaggcagtct cgatgattca cagcgcgcag
     1201 gtgctcgctt cctcggaaca tgtcaggaac tacgtaagcg gagaactcga gaagggcgcc
     1261 gacttccagc tgctgcttca gaagatcaac gccctttggg atgctttcgg acagtcgctt
     1321 gccctggagc ccgtcgagtt tttcgagtcg attctcattg attcccccga cagaaacgat
     1381 ctgcgtctgc tctcgcgtga cccaggcagc aggtttatcg agctgtacaa agagtgggga
     1441 gcggaatttc tgaaagcggt caacaagatc ggggtggaga actacgtctc caccgaatgg
     1501 aagacgagcg acgtcaactg gagcgtactg aacagaagcc ttgattttct tccggacatc
     1561 agcggcaacc ggaacgccgc gcgcggcctg ctctttttga tccaggaggg ggttcctctc
     1621 gacggctttt ctccggatct gatggcgaag atcgccgtaa agaaggatgt ctttctgaaa
     1681 gtatggcagg cgctcactcc tgactccaga aagctcgcgg tcatcttcga gagcggacgt
     1741 cccctcgtta ttcttgagcc gtccctcatc gcctttcccg aagcctgctc gctcttcctt
     1801 cgcgactacg gggtggagtt ctggacctcc tggtcgagcg acgatatctt cgatctcctc
     1861 aggatttcgg aaattcgcgg aaagaacagc gggaaactac gagcggcttc cgtccttccg
     1921 gaggaacgcg cggatctgct cgaaatctac cgccgttccg gggagagggg aattattctg
     1981 tgggacatcc atacgaagga accaacagga gaaatcggca gaaagctcgc ccgtcagagt
     2041 atcgcctgca tggccgaggg gtaccccttc gaggacatga aggatcctga gttcgtccgt
     2101 ttcgccgcag ccacactcaa ctttccggca gggcgatttt tctacgacac cctcaaaggg
     2161 gtgggcagcc ttgtccgcta tcttcttctc ggcggcgtgc tgctgctctt cggcggcgtc
     2221 ttcttcgggc tttttaaacg attccgcggc gcgttcttct ctccgccttc acgctatacg
     2281 atcaaagagg ggacggaaaa aactgaacgg aaggcgctga aagatgtttt tgccgatgac
     2341 gagagagcgg atggaggcgg agccgacgaa acggcttcat ttacagtgga accagaaatg
     2401 atcgaggatc cggaaatttc ccccgcagaa accgatgatg caacggggaa agaggaggat
     2461 ctgcaggaag aggacgccgt atccgcggac gaggagcttc aacgcgggca atccgacgag
     2521 gagagcgcgg gcacggctgg aaaatcaaca cgacagagga gacgcaagaa aaggatgaaa
     2581 aaagagaatg agtaaaaagg gaattccggt cctcacggtc gacgtgattc cgggcaagaa
     2641 gtgcgagtat ctggggtgcg tgtcggcctc ctgctgtctt tcgaagtcgc tggtgcagga
     2701 cgtggcgtcg aacgtgaaga actggactgt cggaggggaa ctgacccggt acaccgatat
     2761 gatcgacgac gcggtcaacc ttgtgacgga gcgcctcggc gtgctggcgc agcagatggg
     2821 agcgaacgcg atcatcggct tccggctctc gacgacgagc gtctccaccg gcgcggcgga
     2881 gcttatcgcc tacggaacgg cggtgcgctt ccccgacctg aactaagcgg gcgccccgcc
     2941 gccggcgcgc cctttccccg gcagccacat gacggtgacg gcaacggccg tgataaggca
     3001 aaagagtgcg aaaaggcgaa atgccaaaag cgtgccgagg agcgtcgagc cgaaccccat
     3061 gtagaggggg agcagaaacc agctcaggtc catggaaaag tgcaccagcg ccgacgcctt
     3121 ggccctgaga cgagccggag cgagatccgc ggtgagcgca agatgcgcgg ggtagccgta
     3181 cccgacgccg attccgtaga ggaatccgga gagcagcatc ccggcaggtg aatcgacgaa
     3241 ggagagccag atgagacacg ctgccatcag caggaacgag gggccggcga agacggttct
     3301 cgggtagcgg ttgaacaggt ttctccctgc tgtacggacg aagagcgccc ctcccgccat
     3361 agcgaagacg aaccatgacg ggacaagccc tttctccagc agcatgctgc cgagaaagac
     3421 aatggaggag tcgcagaggc cgaagaagaa acaggagcag agcatcctcc acgcgggcgt
     3481 ctcccggaag aggtcgcgat aactccccca cttcatggtc gagtttgtct cttcgaagcc
     3541 ggtcgtttgc tttgtgttga ggcgaatcgc gagcataaga gcgagcgccg acgcggcaac
     3601 cggaagaagc aggtagattc tgccgaggtt gttccggata agaaagtcgg tcagcgggac
     3661 gatagtgaac atggggacga tgctgccgat ggcgaccagg gcaaatgcta gtcccctttt
     3721 cgcttcttcc acggcgagcg cctggtaggt tgtaagcgcg accatgaaga cgctgaagcc
     3781 gattcccatg acgatacgaa gcccgacgaa gaaggagaac gaaccctggt agatgtggag
     3841 aaccgctgcg caggcgaggc agagcaaaga cgatgcgatc atgcttcccc tgatcccgaa
     3901 tcgctccgtg atccagcttc ccacggggcg gaccgcggtc gtcgccgcgt agaacacgct
     3961 caccagcacg cctgcggatg cgggggagta cccctgtgtg ctcaggtagg gcgccaggaa
     4021 gaagaacgtg ttggagtacg agtgtatcgc gaagttgatg atcatcaggg aaacgatgag
     4081 ccgatccatg gaagcctccg gaagagagat tttgctgacg ctgtttcgcg gcggattgtc
     4141 cgcctgttcg ggagggcgtc gcgcggaggt tagtgtatac cgtaaccgct ttttattcaa
     4201 tctcctgtat cgtaaagaaa acgcccctcc gcggaatgga ggggcgtttc gaaaaaacgt
     4261 gttcgtaacg agatcagaga cttactactg ggtgatcgcg ctcacgggac agacggacac
     4321 gcagcttccg cactcgatgc aggactcgtc aacgagagcc ttgccgtcga ccatcgaaat
     4381 agcctcgacc gggcaggtgc ctacgcaggt ctcgcaaccc acgcatgcat ccttatcaac
     4441 aactgccttt gccataaata cattcctcct cagcaaggga tattctgacg ctattgaacg
     4501 acttggatta tacatcactt tcttacctcg ggcaactttt gacgcaactc cgtgaataac
     4561 cggagatacg gaggtcaccc cctcccgctt ccccagagca tcgcgggcgg gtactccgcc
     4621 ttcgggtcgc gggcgatcag ccgttccagc tcatcctgag gagaagagcc ttcgtagaga
     4681 aggcggtgca ctccctgcga gaccggaagt tccacggaga gcgcgagcgc gctttcagcg
     4741 acggcgcgga ccgtgaacgc tccttcggcg acctgaccga cctcaagttt cgcttcatcg
     4801 agagaaaggc cccggccgag cgcgagccca aggcggaagt tgcgcgactg cgtgctgtag
     4861 caggtgagga c
//
LOCUS       DAOPKU010000189         4864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18922_length_4864_cov_4.692244, whole genome shotgun sequence.
ACCESSION   DAOPKU010000189 DAOPKU010000000
VERSION     DAOPKU010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18922_length_4864_cov_4.692244"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PK849_07080"
     CDS             complement(<1..172)
                     /locus_tag="PK849_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HPE65926.1"
                     /translation="MAGTIRSIREQVALYKGLTVRYRTAKGRRKVEERQGVILETYPN
                     LFTLYVESQDSKV"
     gene            complement(400..2394)
                     /gene="selB"
                     /locus_tag="PK849_07085"
     CDS             complement(400..2394)
                     /gene="selB"
                     /locus_tag="PK849_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPE65927.1"
                     /translation="MEGREISLVLGTAGHIDHGKTTLVKAVTGVDCDRLIEEKKRGIT
                     IELGFAPLQLDDGRIVSIVDVPGHERFIRQMVAGASGIDGVLLVIAADEGVMPQTREH
                     LEILSILGVKDGFVALTKTDAVDEDFLELALEDVRDFLTGTFLEGKPIIPVSAIKGSN
                     IGLLREELGRLVDRIRPRPRKGPFFLPIDRSFPMSGFGTVLTGTAYRGELSAGNEGIV
                     LPSGREGRIRSVQVHGADSKVAWAGQRVAVSLAGVSIDEISRGDVLCAKGIYAPTRCF
                     ECELHLLESVPSLKHWQRVRLHIGTSDVTARVSLLESPSIQSGDTVPAQIVTEEDVVC
                     LLDQRFVIRRYSPLETIAGGKVLSPFASKPRGRNARAACIKRIKSLMHAATPAERLAI
                     LIDSAGILHLADAVRLLQETPEEVVRIGSKLRPAGGQTSKQTSSESTGQHPVDEHPIF
                     LQGERKFFLSGRSVARLSSEISVFLQTFHQSHPSQKGALLDEVVLSVLKEFDTRTARS
                     FIDFLVEGGTLVMEEGFIRLSAFTPRDDERFSKDSESLLALCRERGFQPPLLEEAPQA
                     LGMDEKRFSSLLKGMRETGKIAIVSGFLLSFEIEEKLLELLIKEKEGITLARVRDLTN
                     SSRKFILPLLEYIDAKGYTRRAGEVRVLLPSKLPRRRETV"
     gene            complement(2430..3848)
                     /gene="selA"
                     /locus_tag="PK849_07090"
     CDS             complement(2430..3848)
                     /gene="selA"
                     /locus_tag="PK849_07090"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPE65928.1"
                     /translation="MNPNEKQTGAFRSLPSMDVLLGSPCVEPFFSMIGRESVKSILTE
                     ALQSERKELLNGRSDHASCESVLARAKPLLERRGAKSLIPVVNATGVVVHTNLGRSCL
                     AEEAAEAVLAVARRYNTLEFNLEEGKRGRRTDHVEWLLRRVTGAEAGLVVNNNAGAVL
                     LSLAALCQGKEVIVSRGELVEIGGSFRIPDIMAFSGVRLVEVGATNRTHLRDYANAVT
                     ENTAALLKVHPSNFRMEGFVSSTPREELASLAKDRGLFFIEDLGSGMLIDYSRCGLLG
                     EPTVRDCIEQGVHLVTFSGDKMLGGPQIGGVAGKKQLVDALRSYPLHRALRVDKMTLA
                     AFEATLRLYLRGEEKRIPTVSMLFAESHSLQLRAEALLNTLRSSFPNGQFTLTEVDDA
                     VGGGAFPAEKLPGWGVSVSMEDWGSAGRVQRLLRGAQPPVVAGARENETILHVRTLLP
                     GDEERILEAFRFAETRALRGNE"
     gene            4015..4683
                     /gene="yedF"
                     /locus_tag="PK849_07095"
     CDS             4015..4683
                     /gene="yedF"
                     /locus_tag="PK849_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HPE65929.1"
                     /translation="MGKIDARGRSCPQPVMMTKAHVEKGEDEIEVFLDNAISASNVQR
                     YLEKNGYFVRIFDEDGSIIIRASVITEASLPADNTPATQTADSAALHTPPLPKTVTVS
                     AAPAAARSTASTAVLITKKGIGGDDSLLGEVLMKGFLGTISQMAPLPSVLALMNEGVK
                     LAMKHTSSLDHLMLLEKKGVRILVCGTCTNHFGVTSDIGAGVISNMFEITEALLASDK
                     ILSL"
BASE COUNT         1089 a         1357 c         1267 g         1151 t
ORIGIN      
        1 aaaccttgct gtcctgcgat tcgacgtaaa gagtaaagag attcggatag gtttcgagaa
       61 tgacgccttg acgttcctcg actttgcgac gtcctttggc tgtccggtaa cgaaccgtca
      121 gtcccttgta caatgccact tgttctcgga tcgatcgaat tgttcctgcc atacaggtca
      181 ctcccagcaa ctcatcatta caagaatgtt atcacaaata ctgaaaaata tcaaatatca
      241 tcaggcccga ttgtgacggg actgcaagcc ttctcctgta tggactattt gccttttctc
      301 cggcgaatac cgtagagcgt cacacgagaa aagtcgcttg gcggcatgtc gttcgcagcc
      361 ggtaccgtcc tctccgggtg gcgttccgca tttcatcctt cagacggttt ctctccttcg
      421 cgggagtttt gagggaagta aaacccggac ttctcccgct ctcctcgtgt accccttcgc
      481 atcgatatac tccagcaggg gaaggatgaa ctttctggag ctgttcgtca gatcccgaac
      541 ccttgcaagc gtgatgcctt ctttttcctt aatgagaagt tcgagcagct tctcttctat
      601 ttcgaacgag agaaggaagc ccgaaacgat ggctatcttg cctgtttctc gcattccttt
      661 gaggagcgag gaaaaacgtt tttcgtccat gccaagagcc tgaggcgcct cttcaagaag
      721 cggaggctgg aatccacgct ccctgcagag cgcaagaagc gattccgagt ctttggaaaa
      781 gcgttcatcg tcacggggag taaaagcgct caagcgaata aacccctcct ccatgaccag
      841 tgttccgcct tcaacaagga aatcgataaa agagcgcgcc gtacgcgtat caaactcctt
      901 gagcacgctg agaacgacct cgtcgagcag agcgcctttc tgcgaggggt gcgattggtg
      961 gaatgtctgt aagaaaacgg aaatttccga ggagagacga gcgacgcttc gccccgagag
     1021 aaagaacttt ctctctccct ggagaaaaat cgggtgctca tccactggat gctgtcccgt
     1081 tgattcggaa gacgtctgtt tggaggtctg gccgcctgcg ggacgaagct ttgaaccgat
     1141 acggactacc tcttccgggg tttcttgaag caaccggacc gcgtcggcaa gatggagtat
     1201 gcctgccgag tcgatcagaa ttgcaaggcg ttcagcgggg gtcgcagcgt gcataagcga
     1261 cttgattcgt ttgatacagg cggcacgtgc atttctcccc cttggtttcg aggcgaaggg
     1321 tgaaaggact tttcctccag cgatcgtttc gagcggactg tacctgcgga tgacaaatcg
     1381 ttgatcaagc agacagacta cgtcctcttc cgtgactatc tgtgcgggga cagtgtcccc
     1441 gctctgtatc gacggcgact cgagaaggga aactctggcc gtcacgtctg aggttccgat
     1501 atggagtcga acgcgttgcc agtgtttcag cgacggcacg ctctcaagca ggtgcagctc
     1561 gcattcaaaa cagcgtgtcg gggcgtagat gcctttggca cagaggacgt ctccgcgcga
     1621 tatctcatca atggaaacgc ccgcgagact tacggctact cgttgccccg cccaagcaac
     1681 cttggaatcg gctccgtgta cctggacgct gcggattctt ccttccctgc cggacgggag
     1741 cacgatccct tcgtttccgg cgctcagctc tcctctatag gcggtgcctg tgagcaccgt
     1801 tccaaagcct gacatcggga atgatctgtc aatggggagg aaaaaaggcc cctttcgcgg
     1861 gcggggccga atcctgtcga cgaggcgccc cagctcctct ctcagaaggc cgatgttgct
     1921 ccccttgatt gccgagactg gaataatagg ttttccctct aaaaacgttc cggtgagaaa
     1981 atcgcgcaca tcctccaaag caagttcaag aaaatcctcg tcgaccgcgt cagttttcgt
     2041 gagcgcaaca aaaccgtcct tcaccccgag aatactgagt atttccaagt gctctctcgt
     2101 ctgcggcatg acgccctcgt ctgcggcgat cacgagaaga acgccgtcga ttccggaagc
     2161 tcccgcaacc atctggcgga tgaaccgttc gtgccccggt acgtccacaa tgcttacaat
     2221 acggccgtca tccagttgca gaggagcaaa accaagttca atcgtgatgc cgcgcttttt
     2281 ttcttctatc aatcggtcgc agtcaacgcc cgtcacggcc tttacgagcg ttgtcttccc
     2341 gtggtctata tgaccggcag tacccagcac cagagagatt tctctgcctt ccacattctt
     2401 catcctctct ccatcttcgt tgcgctctcc tattcgttgc cccgaagagc tcttgtttct
     2461 gcgaaacgaa acgcttcgag aatccgttct tcgtcccctg gaaggagtgt acgcacatga
     2521 agtatagttt cgttctcacg tgctcctgca actaccggag gttgtgctcc cctaagaaga
     2581 cgctgtaccc ttcctgcact cccccagtct tccatggaaa cggaaacgcc ccagccggga
     2641 agcttctccg ccggaaatgc gcctccgcct accgcatcgt ccacctccgt cagcgtaaac
     2701 tgcccgttcg ggaaggacga ccgaagggtg ttgagcagag cttccgccct caactgtaag
     2761 gaatgcgatt cggcgaagag cattgatacc gtggggattc gtttttcttc tccccgcaga
     2821 tagagccgca gcgtggcctc gaatgccgca agggtcattt tgtccacccg gagcgcgcga
     2881 tgcagcggat aggatctcag ggcgtcaacg agctgtttct ttcccgccac tcctccgatc
     2941 tgcgggccgc caagcatctt gtcgcctgag aaggttacaa ggtgcactcc ctgttcaata
     3001 caatctctca ctgtaggttc ccccaacagc ccgcatcggg aataatcgat caacatcccg
     3061 ctgccgaggt cctcgatgaa gaaaagtccc cgatcctttg cgagagaagc cagttcttcg
     3121 cgcggcgtag aagaaacaaa accctccatg cggaaatttg aaggatgtac cttgagcagc
     3181 gccgccgtat tctccgttac agcgttcgcg tagtcccgca gatgcgttct gttggttgcg
     3241 ccgacctcta caaggcgaac gccggaaaat gccatgatat ccgggatacg gaatgatccg
     3301 cctatctcca cgagttctcc ccgggagaca atcacttctt tcccctgaca gagcgctgca
     3361 agagagagaa ggacggctcc tgcgttgttg ttgacgacga gccccgcttc tgcgccggtg
     3421 acgcgccgaa gaagccattc tacgtggtcg gttcgccgtc ccctttttcc ttcttccaaa
     3481 ttgaattcaa gcgtgttgta gcggcgcgct actgccagca cggcctctgc cgcttcctcc
     3541 gcaagacagg aacggccgag gttggtgtga acaacaaccc ctgtggcgtt tactacgggg
     3601 attaaacttt tcgctcctcg tcgttccaaa aggggtttcg cgcgcgcgag cactgactcg
     3661 caggaggcgt ggtcagaacg tccgttgagt aattcttttc tttcggactg gagcgcttct
     3721 gtgagtatgg actttacgga ctcgcgcccg atcatcgaaa agaaaggttc cacgcacggc
     3781 gaaccaagaa gaacgtccat tgaaggaagc gagcgaaacg cccctgtttg tttttcgttc
     3841 ggattcacga gaaatcattc acctttccat cgtcccgtag tgtggacgaa cagctatttc
     3901 aaatataatt tgtccttgtt tctattctac attataattg gtccgacgga gagcaacact
     3961 gtgtcattat ttcctccgcg ctttctaaaa agcgcggggt agggaggcag tagaatgggg
     4021 aagatagacg cacggggcag gagctgcccg caaccggtca tgatgacgaa ggcgcatgta
     4081 gaaaaagggg aagatgagat cgaagttttc ctcgacaatg cgatttccgc atccaatgta
     4141 cagcgatatc tagaaaaaaa cggctacttc gtccggattt tcgatgagga cggatcgatc
     4201 attattcgcg cttccgtgat taccgaagcc tcgctgccgg ccgacaacac gccagcaacg
     4261 caaacggcag atagtgctgc gctgcatact cctccgcttc cgaagaccgt aactgtctcc
     4321 gctgcaccgg cggcggctcg ttctacagca tcgacagctg tactcatcac aaagaaagga
     4381 ataggaggag acgattccct cttgggggag gttcttatga aggggttcct tggaacgata
     4441 tcccagatgg ctccgcttcc ctcggttctc gccctgatga acgaaggcgt aaagcttgcg
     4501 atgaagcata cttcttcttt ggatcatctg atgcttctgg agaaaaaagg cgtccgcatt
     4561 cttgtctgcg gaacgtgtac gaaccacttt ggcgttacat cggacatcgg cgccggggtg
     4621 atctccaata tgttcgaaat caccgaagcg cttctcgctt cggacaagat tctttctttg
     4681 taggatactg ctcaataggc gctcctcgtg tcgttatcga agagcgccta ttgttttttg
     4741 aaagatggtt atattcccgg ggattcttct ctcgaaggaa gacaaaagag gagatattcg
     4801 gcaagaaggg agagaacttc agcagaggtt ttttcggggc tgagaaaatc gagcacctcg
     4861 tcgt
//
LOCUS       DAOPKU010000190         4854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_18960_length_4854_cov_5.267972, whole genome shotgun sequence.
ACCESSION   DAOPKU010000190 DAOPKU010000000
VERSION     DAOPKU010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18960_length_4854_cov_5.267972"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PK849_07100"
     CDS             <1..748
                     /locus_tag="PK849_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65930.1"
                     /translation="VYRSDGRTWTSYGFPAKATDPSVISPQHGAPELDPVIAMAESGG
                     NVFAATWRNGLLRSRDGGRSWEHTEGNDGVYEITPSHSVTAEYRYVYALSSDGGRVAI
                     ATDRGFSASVDGGETWKNSYVNSGLFSVLFDREGTLWGVSRNGVWRCTPEDMNVEELE
                     IEGFEWSPFSYFTDLFRGSGYAVFGVKDDLLHRLLPKDGGYVMERASLSNVKVLAMLP
                     LADGGILMGTSKAFYRSDDGGSTWKEIGLP"
     gene            783..1973
                     /locus_tag="PK849_07105"
     CDS             783..1973
                     /locus_tag="PK849_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65931.1"
                     /translation="MRELYRRFGALLLLFLAVTFFLAVRDDVIRLYAVDMAEEYRSAY
                     LPVEGWGASTAMRGVVRSSKRISSVAAFIDAHTEGRLEVVEGKSWEEIFAGVLAPPET
                     SPLPDRRASDTWRASYYFLPKEDPFPEVFREMKSEDQAFRFLKLSAPSGPRFIGMTVQ
                     GPEDAMSAGAPSWILYPFRRFAAVPLLLALFVYLIFPPRKKAPAGALVYPKWSSWILP
                     DLMGLLFSCIFFAFPFFLAPAIFGSGNVLNIADGGIILTLVFWLMAAIFASILYWSAK
                     YASFSLELLPGAMMLRTLGKEQRIPYIAIESAGIADYRPPKWLRTTLFIASLGSWRML
                     GHALLLSSRSDWGIELRMKHGDPVKFLCSNLPGAERIIEALRREGVNISPELESVLSE
                     RDDR"
     gene            complement(2114..3250)
                     /locus_tag="PK849_07110"
     CDS             complement(2114..3250)
                     /locus_tag="PK849_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65932.1"
                     /translation="MKNMIVVVCALLAFGAFLVSARPANAGEPSVSVDYEYGDLKEVI
                     VGLPYGMSPSLEAKWFADALKVLPDDEAEYARKTAGMLWTEMINPETGKSETQMLEEE
                     NQAFIAILESLGVKVYRPAEITVGFIKKHYGADVLLNGFSQDFPRDNMAVIGNNVIEF
                     NLRTAIRKVDISGFRDILSEKCSDSTVKWFSMPHTELLEPARADTPFLEGGDVIVLGK
                     TVFVGNTENPSVGSNEAGYKWLKNIVGDRYTVKRIRLVESVLHLDCVLSVPRSGLAIV
                     CEEAFIDGLPEEIKDWDLIRVSLDDVKRLAVNGLPVDSQNYVLSYNLHNDNRFIQTEL
                     ERRGIKVHRVFFGTHNGQGGSLRCATQPLKREVKTAKTHESQGK"
     gene            3640..3915
                     /locus_tag="PK849_07115"
     CDS             3640..3915
                     /locus_tag="PK849_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter permease"
                     /protein_id="HPE65933.1"
                     /translation="MAVHMTRSNEIQGSAAGRLLEMLLPRPTSTFLLRCADGPDSILV
                     VDRSLPPSEGKKAVLSTEKGLRLATLGSAFDSSSVWGVVTWVIREPS"
     gene            3891..>4854
                     /locus_tag="PK849_07120"
     CDS             3891..>4854
                     /locus_tag="PK849_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HPE65934.1"
                     /translation="MGHPGAFLKPCEELLALCDCNNFFVSCERLFRPDLEGRPVVVLS
                     SNDGVIISRSNEAKTLGIKMGEPYFRVRAFLERRGGAVFSSNFQLYEEISARVMEVLR
                     RSTPSIEVYSVDEAFLELPPAARERPVEWGRELRAAVLKETGIPVSVGVAPAKTAAKL
                     AAERGKKRPETGGVFALLPGEAWDDILRDVPVEDVWGIGRQWSDFLRRRGVSTALRLR
                     DTPDDWLEKYLGVRGLRTAWELRGIRCFPIEEYEKPQKSIHSSRSFSTPVRSRESLAE
                     AVTEFALTAGRRLRSQRSLAGCLSVSISTSRFRSPFYGAREEVVL"
BASE COUNT          864 a         1512 c         1476 g         1002 t
ORIGIN      
        1 cgtgtaccgt tccgatggga ggacgtggac gagctacggc ttccccgcaa aggcgaccga
       61 cccgagcgtc atctcacctc agcacggcgc gccggagctc gatccggtga ttgcaatggc
      121 cgagagcgga ggcaacgtgt tcgcggcgac gtggcggaac ggccttctcc gctcgcgcga
      181 cggcggacgg agctgggagc ataccgaggg gaacgacggc gtctatgaaa tcactccttc
      241 gcactccgtc acggcggagt acaggtacgt ctacgcgctg agttcggacg gcggccgcgt
      301 cgccatcgcc accgaccggg ggttctccgc ctccgtcgac ggcggggaga cctggaagaa
      361 ctcctacgtg aactccgggc tcttctccgt cctcttcgac cgggaaggga cgctctgggg
      421 agtgtcccga aacggcgtct ggcgctgcac cccggaggat atgaacgtcg aggagttgga
      481 gatagaggga ttcgagtggt cgccgttcag ctatttcacc gacctcttcc gcggaagcgg
      541 ctacgcggtg ttcggggtaa aggacgactt gctgcaccgg ctgcttccga aggacggcgg
      601 gtacgtcatg gagagagcgt ctctctcgaa cgtcaaggtg ctcgccatgc ttccccttgc
      661 ggacggaggc attctgatgg ggacgtcgaa ggcattctat cgctcggacg acggcggaag
      721 cacgtggaag gagatcgggc ttccatgagc ggcccggact ctttgaagag gaggcgactg
      781 gtatgagaga gctgtaccgc agattcggcg cgcttctgct gctttttttg gccgtgacgt
      841 tcttcctcgc cgtccgcgac gacgtgatcc gtctctacgc cgtcgacatg gccgaggagt
      901 accgctccgc gtatcttccc gtggaggggt ggggggcgtc caccgcgatg cggggcgtcg
      961 tccgttcgtc gaagcggatt tcctccgtcg ccgcgttcat cgacgcgcat actgaggggc
     1021 ggcttgaggt cgtcgaagga aagagctggg aggagatctt cgcgggcgtc ctcgcgcctc
     1081 ccgagacgtc gcccctgccg gacaggaggg cgagcgacac ctggcgggcc tcctactact
     1141 tcctcccgaa ggaggacccc ttcccggagg tgttccgcga aatgaagagc gaggatcaag
     1201 ccttccgttt cctgaagctc tccgcaccgt cgggtccgcg cttcatcgga atgaccgtcc
     1261 aggggcccga ggacgcgatg agcgcgggcg cgccgtcgtg gattctgtac cctttccggc
     1321 gctttgccgc cgtgccgctg ctcctcgcgc tcttcgtcta ccttattttc ccgccgcgaa
     1381 agaaagcgcc cgcgggcgcg ctggtgtacc cgaagtggtc gtcctggatc ctccccgacc
     1441 tgatgggatt gctcttctcc tgcatcttct tcgcgtttcc gttcttcctg gcgccggcga
     1501 tcttcggcag cggcaacgtg ctgaacatcg ccgacggcgg gatcatcctc acgctcgtct
     1561 tctggctgat ggcggcgatt ttcgcctcca tactctactg gtcggcgaag tacgcctcgt
     1621 tctcgctcga actgcttccc ggcgccatga tgctccggac gctcggcaag gaacagcgga
     1681 ttccgtacat cgccatcgaa tccgccggta tcgccgacta ccgccctccc aagtggctgc
     1741 ggacgacgct cttcatcgca tccctgggaa gctggcgcat gctggggcac gcgctgctcc
     1801 tctccagccg gagcgactgg gggatcgagc tgcgcatgaa gcacggcgat ccggtcaagt
     1861 tcctctgctc gaacctgccg ggcgcggagc ggatcatcga agccctccgg cgcgagggcg
     1921 tgaacatctc gccggaactg gagagcgtgc tctccgaacg tgatgatcga tagaatgcaa
     1981 aaaacgggag aggcgcaacg ccctcccgtt tttcatgtac tcgtctttta aggttctcga
     2041 tgggggcgca atccggcgca gccttcaccc cgaaacggat ctgcccatcg gcaggacgct
     2101 gtttccgttg ccttcacttc ccttgacttt cgtgcgtttt cgccgtcttg acctctcgct
     2161 tcagaggctg ggtcgcgcag cgcaacgatc caccctggcc gttgtgggtt ccgaaaaata
     2221 cgcggtggac tttgatgccc cgcctttcga gctcggtctg gatgaaacgg ttgtcattgt
     2281 gcaggttata cgacagaacg tagttttggg aatcgaccgg aagcccgttg acggccagtc
     2341 gcttgacgtc gtccagactc actctgatca ggtcccagtc cttgatctct tcgggcaagc
     2401 cgtcgatgaa ggcctcttcg cagacgatgg caagcccgct gcgcggcacg gaaagcacgc
     2461 aatccagatg cagcacgctt tcgacgagcc ggatgcgttt caccgtatag cgatcgccca
     2521 caatgttttt gagccacttg tagccggcct cgttggatcc cacgctggga ttttccgtat
     2581 tgccgacgaa aacggtttta cccagcacga tgacgtcgcc gccctcaaga aacggcgtat
     2641 ccgcgcgggc gggttcgaga agctccgtgt gcggcatgga aaaccacttg accgtcgagt
     2701 cggaacactt ttcggacaga atatcccgga atccggatat atccaccttg cggattgccg
     2761 tccgcaaatt gaactcgatg acgttgttgc cgatcacggc catgttgtcg cggggaaaat
     2821 cctggctgaa accgttgagc agtacatcgg cgccgtaatg ctttttgatg aagccgacgg
     2881 taatctccgc cggacggtac actttgacgc ccagagactc taaaattgcg atgaacgcct
     2941 gattttcctc ttccagcatc tgcgtctcgc ttttccccgt ttcgggattg atcatctccg
     3001 tccagagcat ccccgccgtc ttccgggcgt actccgcctc atcgtccggc agcactttca
     3061 acgcgtcggc aaaccatttg gcttccagcg acggcgacat gccgtacggc aggccgacaa
     3121 tcacctcttt cagatcgccg tattcgtaat ccacggaaac ggaaggttcc cccgcgtttg
     3181 cgggtcgggc ggaaaccaga aacgctccga acgccaggag cgcacataca accacgatca
     3241 tatttttcat gacatctctc ctcgtgagtg gaaaaaatct ttccttcctt catatcggca
     3301 cgcctcaact ccccggcgca tacacaggac acgatgatgt tccggcagcc gctttctcat
     3361 gtccggcggc aacagataca tcggagcgct gccgctgctt ttgaaattat acaccgcaat
     3421 gatccgcccg cagcagtctt tacagcgtca taccctctcg gaacgcactt ccgtctgtga
     3481 atactctcaa ccagcgtttc ctccgcgaca ggctctcaaa tagatacttt aagaaatcta
     3541 tacaaggtat tgactttcta tgtgtatatt attatactga cacgcaaaga ctcactgtaa
     3601 tcattataca caaaaacagt ctgaaggaga gtgcattcta tggccgttca catgacgcga
     3661 agcaacgaga ttcagggcag cgcggccggt cgcctgctgg agatgctgct tccccggccg
     3721 acctccacat ttcttctgcg gtgcgccgac gggcccgact ccatcctcgt ggtggaccgc
     3781 tcgctccccc cttcggaggg gaagaaggcc gtcctctcca cggagaaggg gctcaggctc
     3841 gccacattgg gctccgcgtt cgattcgtcc agcgtgtggg gggtggtaac atgggtcatc
     3901 cgggagcctt cctgaagcct tgcgaggagc tgctcgccct ctgcgactgc aataacttct
     3961 tcgtctcgtg cgaacggctc ttccgccccg accttgaggg gcgtcccgtg gtggtgctct
     4021 ccagcaacga cggggtgatc atctcccgct ccaacgaggc gaagactctc ggtatcaaaa
     4081 tgggggagcc ctactttcgg gtgcgcgcct ttctggagcg gcgcgggggc gcggtgtttt
     4141 ccagcaactt tcagctctac gaggagatat cggccagggt catggaggtc ctccggcgga
     4201 gcacgccgtc catcgaggtc tattccgtgg acgaggcgtt tctggagctt ccgcccgcag
     4261 cgagggaacg cccggtcgaa tgggggcgcg aactccgggc cgccgtgctg aaagagacgg
     4321 ggattcccgt ctcggtgggc gtcgctcccg cgaagacggc ggcgaagctg gccgccgaac
     4381 ggggcaagaa gcggccggag acgggcgggg tcttcgccct tctgccggga gaggcatggg
     4441 acgacattct ccgggatgtt cccgtggagg acgtgtgggg gatcgggcgg cagtggagcg
     4501 acttcctccg cagacggggc gtgtccacgg cccttcgcct gcgggacacg cccgacgact
     4561 ggctggagaa gtatctcggc gtccgggggt tgcggacggc gtgggagctg cgggggatac
     4621 gctgttttcc catagaagaa tatgaaaaac cccagaagtc catacactcc tcccgctcct
     4681 tctccactcc cgtccggagc cgggaatctc tcgccgaggc ggtgacggag ttcgccctta
     4741 ctgcggggcg gcggctccgc tcccagcgct ccctggcggg atgcctctcc gtgagcatct
     4801 ccaccagccg cttccggagc cccttctacg gcgccaggga ggaggtcgtc ctgg
//
LOCUS       DAOPKU010000191         4837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_19049_length_4837_cov_3.198034, whole genome shotgun sequence.
ACCESSION   DAOPKU010000191 DAOPKU010000000
VERSION     DAOPKU010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19049_length_4837_cov_3.198034"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PK849_07125"
     CDS             <1..282
                     /locus_tag="PK849_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HPE65935.1"
                     /translation="GGSQIVIFTTGRGSPTGFPLAPVIKITGNPKTYVMMEENIDINA
                     GTIIDGVESIPEVGERVFAEILQTCNGKLTKAEALKHMEFGIYKLTSTF"
     gene            328..1317
                     /locus_tag="PK849_07130"
     CDS             328..1317
                     /locus_tag="PK849_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HPE65936.1"
                     /translation="MGGKVPILKTIQKVPGGLMVVFLLLGAIVNTFAPKSLMIGSFTT
                     ALFKQGALPLIAVLLFCSGAQITIKTAGVALWEGFVLNTVKILLGISIGLVVGKLMGP
                     EASLFGLTPLALIAAMANSNGGLYTALAQKYGDASDIGAVAIISTNDGPFFTMVAMGM
                     TGLANIPIIAMVAVVVPIVIGMILGNLDEDMREFLAPGVMLSIPFFSFPLGAGLNLMD
                     IAKAGIPGIALGLMTLLVTGIGGFFAYKYLIPSREKRSDAVGASIGTTAGNAAGTPAA
                     IAAVDPTWAPYAAAATVQVGASIVITAILCPLLVDFLSRICKQHNNVAKEEGN"
     gene            1416..2705
                     /locus_tag="PK849_07135"
     CDS             1416..2705
                     /locus_tag="PK849_07135"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HPE65937.1"
                     /translation="MPAAVRTADGMSYNRKERNGGRHMNARYIVVADDFTGSNDTGIQ
                     FLKAGYDASVILDPSALSTIEGERVVVVDTESRNIPAQEAVACLKTVAEYLLPLRGKR
                     VIYKKVDSTLRGNIAAETAVLRETLGFSVTAFTPAYPRNKRTVREGLLLLDGIPVADT
                     EMGKDPRKPVTTSSLQETLRGGGSLEVRNVSLAEIRAGEIPEILRAAGNAGSYSFDVE
                     TDGDLELIVNGMCAALPPEDILWVGSAGMAEALVSRSSPAFFVVGSMSPRSALQARKL
                     LQEKDVAPVLVNIRALLHDVASEERRLSHTVSGLLRSGRSVLLASSLDEEQIEEGRNA
                     NASETICRSLAAVVPSVLKSARVSGMFITGGEVAIWIVRALGAGGTDLVKEIEPGIPL
                     VRLRGGEYHGLPVVTKAGAFGGERTMAECFSVLTDAK"
     gene            2725..3990
                     /locus_tag="PK849_07140"
     CDS             2725..3990
                     /locus_tag="PK849_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPE65938.1"
                     /translation="MTDVRQDALKIAQKAIEAVLPENAVEAALRGEAFSARARSGGRI
                     VLTAIGKAAWRMAKAAADTLGDRLDGGVVVTKYGHSNGPIKGIEIFEAGHPFPDENTI
                     KGTARALERVSGLSAEDTVLFLVSGGGSALFEKPREGITLDDLVGVTDQLLACGADIV
                     EINTIRKRLSSVKGGRFAQFVAPAHVFSVVLSDVLGDRLDSIASGPAYPDGSTSADAS
                     RIVEKYGLSLSTVLQEALEKETPKSLTNVTTVITGSVTALCKAAEREAAALGYTPLLL
                     TTMLNCEAREAGAFIAAMAKEIRSSGNPASPPCALLLGGETIVRLKGKGKGGRNQEMA
                     LAAAFGIKGLADTVVISVGSDGTDGPTDAAGGVVDGGTIGRLADLGLDAEALLADNDS
                     YHALDACGGLVKTGPTGTNVNDLTIVLCR"
BASE COUNT          945 a         1393 c         1560 g          939 t
ORIGIN      
        1 ggaggctccc agatagtgat tttcactacg gggcgcggct cgccgaccgg cttccccctt
       61 gctccggtga tcaagatcac gggcaatccc aagacctacg tcatgatgga ggagaacatc
      121 gacatcaacg cggggacgat catcgacggc gtcgaatcca taccggaggt gggggagcgc
      181 gtcttcgcgg agatcctgca gacctgcaat ggaaaactca ccaaagcgga agcgttgaaa
      241 cacatggagt tcggtatcta caaactgacg tcgacttttt gaagaggctc ctatcccaaa
      301 tgacttttta aaaggaaggt gcatactatg ggcggtaaag ttccgattct caagacgata
      361 cagaaggtgc ccggcggact catggtggtc ttcctgcttc tcggcgcaat tgtgaacact
      421 tttgccccga agtcgctcat gatcggcagt tttaccaccg cgctctttaa acaaggagcg
      481 ctccctctca tcgcagtgct cctcttctgc agcggcgcgc agatcacgat caagacggcc
      541 ggggtcgctc tctgggaggg gttcgtgctg aacaccgtca agatccttct tggcatttcg
      601 atagggcttg tcgtggggaa gctcatgggg ccggaggctt ctcttttcgg tcttaccccc
      661 ctcgccctca tcgcagccat ggcgaactcg aacggtggcc tctacaccgc gctcgcccag
      721 aagtacggtg acgcctccga catcggcgcc gtggccatca tctccacgaa cgacggtccc
      781 ttcttcacca tggtggccat ggggatgaca gggttggcca acatacccat catcgcgatg
      841 gtggcggttg tcgtcccgat cgtcatcggt atgatccttg gcaatctcga cgaggacatg
      901 cgggaattcc tcgccccggg tgtcatgctc tccatcccct tcttctcttt cccgctcgga
      961 gcggggctca acctgatgga tatcgcgaag gccggaatcc ccggcatcgc gctcggcctt
     1021 atgaccctgc tcgtcacggg aataggcggc ttctttgcct ataagtacct catcccgtcg
     1081 agagagaagc ggagcgatgc ggtcggcgcg agtatcggca caacggcagg caacgccgcc
     1141 ggaacgcctg cggcgatagc ggccgtcgat ccgacatggg ccccgtacgc ggctgcggcg
     1201 acggtgcagg tcggtgcgtc gattgtcatc accgcgatcc tctgtccgct gctggtcgac
     1261 ttcctcagca ggatctgcaa gcagcacaac aacgtcgcta aagaagaagg caactgattt
     1321 cagcgaaccg gtccgccctg cgtcgcggtt gccgggctca acccgtgcgg cggagagaga
     1381 gtggtctctt cgccgccgag gagatcgacg aaatcatgcc ggccgcagtc cgcaccgccg
     1441 acggcatgtc gtacaaccgc aaggaaagaa acggggggag acatatgaat gcacggtata
     1501 tcgtcgtcgc cgatgacttc accggttcga acgacactgg aattcagttc ctgaaggcgg
     1561 ggtacgacgc ttcggtgatc ctggatcctt cggcgctctc cacgattgag ggcgagagag
     1621 tcgtcgtcgt ggataccgag tcgcggaaca ttcccgctca ggaggcggtc gcctgtttga
     1681 agacggtggc ggaatacctg ctgcctctgc gagggaagag agtgatctac aagaaggtcg
     1741 actcgacgct gcgaggcaac atagccgccg aaacagccgt cttgcgggag acgctcggct
     1801 tttccgtgac agcgttcacc ccggcctacc ccagaaataa acgcaccgtg cgggaaggtc
     1861 tgctgctcct cgacggaatt cccgtggccg ataccgagat ggggaaggat ccgcggaagc
     1921 cggtgactac ctcgtcacta caggaaacgc tgcgcggcgg cggttcgctc gaggtccgga
     1981 acgtctcgct cgccgaaatc cgggcagggg agattccgga gattctgagg gctgcaggca
     2041 acgccggctc gtactccttc gacgtcgaga cggacggcga tcttgagctg attgtgaatg
     2101 gcatgtgcgc cgcgctgccg cccgaggaca tcctctgggt ggggtcggca gggatggccg
     2161 aggccctggt gagccgaagc agtccggcgt ttttcgtggt cgggagcatg agcccgagga
     2221 gcgccctcca ggcacggaag cttctgcagg agaaggacgt cgctcctgtt ctggtgaata
     2281 tccgtgcgct gctgcacgac gtcgcctccg aggagcggcg cctctcccat accgtctcgg
     2341 gattgcttcg gagcggcagg agcgtccttc tggcgtcatc gctggacgaa gagcagatcg
     2401 aggaggggcg gaacgccaat gcgtccgaaa caatctgccg gagtctcgcc gctgttgttc
     2461 cgtcggtcct gaagtctgct cgcgtctccg ggatgtttat caccggaggg gaagtcgcca
     2521 tctggatcgt ccgggcactg ggagccgggg gaaccgacct tgtgaaggag attgagcctg
     2581 gaattccgct cgtccgcctc aggggaggag aataccacgg ccttcccgtg gttacaaagg
     2641 ccggcgcgtt cggcggtgaa cggacgatgg cggagtgttt ctccgtactg acggacgcga
     2701 aataaaaacg gaggaggaga atgaatgacg gatgttcgtc aggatgcctt gaaaattgca
     2761 cagaaggcca tagaggccgt gctcccggaa aacgctgtgg aggcggcgct gcgcggtgag
     2821 gctttttcag cccgtgcgag gtccggaggg aggatcgtgc tcacggccat cggcaaagcg
     2881 gcgtggcgga tggcgaaggc cgccgctgac acactgggag accggctgga cggaggcgtc
     2941 gtcgtgacga agtacgggca cagcaacggc ccgataaagg gtatcgagat ttttgaggcg
     3001 ggacacccgt ttccggacga gaacacgatt aagggcacag cccgtgcgct cgaaagggtc
     3061 agcgggctct cagcagagga tacggtgctt ttcctcgtct ccggtggggg ttcggccctc
     3121 ttcgagaagc cgagggaggg gataaccctc gatgatctgg tcggagtgac cgaccagctg
     3181 cttgcatgcg gcgcggacat cgtcgagatc aatacgatac ggaaacggct ttcttcggtg
     3241 aaaggagggc gtttcgccca gttcgtcgct ccggcgcacg tcttttcggt ggtactctcg
     3301 gacgtccttg gcgacaggct cgacagcatc gcctccggac cggcgtatcc tgacggttcg
     3361 acgagcgccg atgcgtcgag gatcgtcgaa aagtacgggc tatcgctgag cacggtgctt
     3421 caggaggctt tggagaagga gaccccgaaa tcgcttacaa atgtcacaac ggtcatcacc
     3481 gggagcgtga cggcgctctg taaagctgcg gagcgcgagg ccgccgccct cgggtatacg
     3541 ccgctcctgc tgacgacgat gctgaactgc gaggcacgcg aggcgggggc cttcatcgcc
     3601 gccatggcga aggagatccg gtcgtccggg aatccggcgt cgccgccgtg cgccctgctc
     3661 ctcggcgggg agacgatcgt ccggctcaaa gggaagggaa agggcggacg gaaccaggag
     3721 atggccctgg ccgcggcgtt cggcatcaag gggcttgcgg atacggttgt gatctccgtc
     3781 ggttccgacg gcaccgacgg accgacggac gcggcgggag gcgttgtgga cggcgggacg
     3841 atcggcagac ttgccgacct tgggctcgac gcggaggcgc tgctcgccga caacgactcc
     3901 taccatgcgc tcgacgcctg cggcggcctt gtcaaaacgg ggccgaccgg aaccaacgtg
     3961 aacgacctca ccattgtgct ctgcagatag ccgctcactg cgaaagacgg aacgaggccc
     4021 ccttcttttg ggggcctcgt atgattttct cttgcagtcg ccggggagag actgcctgtc
     4081 gggcttttcc ctggaatctg cagcagtcgc ctccccgtcg gccttgcctg cggaaaggcc
     4141 ggcgtcgctc ccgttctctg tgcctctacg agggagaaga ggaaggcctg ttgaaatatg
     4201 tcctgttcga gatcccgtcg atagggttgt gtctccctaa atccgcaggt ttttcaggca
     4261 gagggagtgt caccggggaa cgcttgggcg gggggtgcag attgtcgtcc ttgacaactg
     4321 cccaagcgat caactcggct ccctgcgttc gccggttgcc tgcttgtgaa accgacatcc
     4381 gtgtcggttt ctctcccccg ggcgcccttc cccaggggat aatctcttcg cccctgggga
     4441 gggcgaattc accttatgcc cggcgacact tcctctgcct ggaaaacctg cggattgacc
     4501 aactgccgta ttgccacaaa ggaataaacc tgatatcata acggacatga gaaaaaaggc
     4561 catcacttta ctagggttta atgcttttgt gtgttgttgc gcggagggtt gtttcacata
     4621 atccgccgcc acatgtttcg tgccgcggtg gaacaaagct gcggcgttcg accatcgccc
     4681 cgtcacaggg gaaaagtatt tttgcgaggg accgcaggaa agaggcggtc cctcgcttca
     4741 tataaggagg cgttccaatt ggggtgtttt caaaagtagc tcgggactat agggcagtgc
     4801 gacaaaacga tccggccata ccgaaggggc ggcgagg
//
LOCUS       DAOPKU010000192         4822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_19122_length_4822_cov_4.585903, whole genome shotgun sequence.
ACCESSION   DAOPKU010000192 DAOPKU010000000
VERSION     DAOPKU010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19122_length_4822_cov_4.585903"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PK849_07145"
     CDS             complement(<1..309)
                     /locus_tag="PK849_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidase"
                     /protein_id="HPE65939.1"
                     /translation="MTDFSVVGKPVLKVDSLSLACGSARYTADYEMRDPLYLAFAYSP
                     VAHGDIVSLDDSEARAMEGVTDVFHAFNTETELFTTAGQGYPEPSPYDTRLFNKRVRY
                     A"
     gene            complement(302..769)
                     /locus_tag="PK849_07150"
     CDS             complement(302..769)
                     /locus_tag="PK849_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPE65940.1"
                     /translation="MKGSFLVNGRTNEWTFEPDATLLDVLRANGHPEVHRGCDEGVCG
                     SCSVLLEGKLVNSCQVFAASAMEKDILTVAGIGTIHEPHPIQTAFVESGAVQCGFCTP
                     AAILATLALLRKNPNPTDEEIHSALDGNYCRCTGYVKIIEAVRLASRRMSSHD"
     gene            complement(766..1593)
                     /locus_tag="PK849_07155"
     CDS             complement(766..1593)
                     /locus_tag="PK849_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPE65941.1"
                     /translation="MSTVQWYFPDTLDEAISYLGREKTVPHGGGTSLIKSGIASLEGL
                     VDLSRLGLDSLKMEKGILEAGAGMTFGGVAESLANLHPECILGKALGASASTPMRNRI
                     TLGGSASLFPLWSDLIGPMSVLHGVICTEGKNGGTYDLPSWLADRTLQKGSLITSLLF
                     PDPVDWLSSYYRETRVGFDYPAFTVSILARKNNSTIDKVRIAVSGGLDRVRRLTELEE
                     RLTGADLSVLASLDIRKMASVEFGRKPAGSPEYLSEIAAVQVERGLKAVLTEEGKSR"
     gene            complement(1709..2803)
                     /locus_tag="PK849_07160"
     CDS             complement(1709..2803)
                     /locus_tag="PK849_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPE65942.1"
                     /translation="MEVAAVVLAAGYSSRMGFFKPLALLNGVSALERILRSMRGAGVR
                     NVLVVTGFNEDVVASAAQRAGARAVYNPRYAEGMFTSVQVGVAALPHSADAFFLLPVD
                     IPTVRVSTLRLMMESASSNGILHPLFRGKRGHPPLIAAALAPHILDFSGEGGLRAFFD
                     GWATWNQDFDVADEGVLMDMDTPQDFEKIHRRLSGLRVPSKMEREVLFDLAGTPLRVR
                     AHCDATASVCLKLAFALRQQGVRIDMPLLHGAALFHDVCRHVPDHVCAGEIFLRRHGF
                     PLLAEIAACHRDLPPLPRIEATLLHLADKLVSDTHIISLDARKKNMRMRYGADQNALL
                     AIRQRYAAARKTARRIERITGQSLETLLQI"
     gene            2978..3556
                     /locus_tag="PK849_07165"
     CDS             2978..3556
                     /locus_tag="PK849_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65943.1"
                     /translation="MNADAYVVLGLKELFRRTDWKCFSYPGTICFPVDLHQPIVPLAD
                     GIGFNGGFLHKNGGDQKRVNLIMLGVFFYQSGVTGGICCGKTRIHSSEVAFDRVRSFS
                     LIPRGPAAQHRMVFSARRERRCKGWLRYKTGEKDQGYQETKNGIISDSHSVAAPFCRE
                     KTAAEGEKENAQWHFPHFWDIVAYTHRQKHFF"
     gene            3570..4136
                     /locus_tag="PK849_07170"
     CDS             3570..4136
                     /locus_tag="PK849_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPE65944.1"
                     /translation="MNLVLETIKKRRSVRSFRPDQITDDELNAIIESGLWAPSGHNTQ
                     PWHFLVVQDKSKIDQMSRSTIEKMANSPIDWVRKLAAKEGYHLFHGAPTVIIVSGRQS
                     GDTFLPVVADCAAAIQNMLLGAESLNIGTCWIGLTSFLFEDSEETASLKIPEGYAPYY
                     SIAVGYKSAGFIPAPPTRKEGTVSTFGK"
     gene            complement(4141..4224)
                     /locus_tag="PK849_07175"
     tRNA            complement(4141..4224)
                     /locus_tag="PK849_07175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4189..4191),aa:Leu,seq:tag)
     gene            complement(4425..4769)
                     /locus_tag="PK849_07180"
     CDS             complement(4425..4769)
                     /locus_tag="PK849_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65945.1"
                     /translation="MFMRDEEGAFPVFSSFLFYFSEIFSFSEVRTEEKIDEKSDDRQQ
                     EEDEQPGDGPVGIALFEKDNARNKDAVCPEKTKGILFEEIHYLLWNAVRRLAPEITVK
                     EQSDIVSDRLRI"
BASE COUNT         1218 a         1301 c         1227 g         1076 t
ORIGIN      
        1 cgcgtaccgt acgcgcttgt tgaaaaggcg cgtatcgtac ggagaaggtt ccgggtatcc
       61 ttgccccgcg gtcgtaaaca actccgtttc cgtgttgaat gcgtgaaaga cgtccgtaac
      121 gccttccatc gcgcgagcct cggaatcgtc gagagaaaca atatccccgt gcgccacggg
      181 cgaataggca aaagcaaggt agagcggatc acgcatctcg taatcggcgg tgtagcgggc
      241 agaaccgcac gccagcgaca gagagtcaac cttgagaacg ggtttcccca caacagagaa
      301 atcagtcatg cgagctcatc ctcctggaag cgagacggac cgcttcaata atctttacgt
      361 agccggtgca ccggcagtag tttccgtcga gggcggaatg aatctcctca tcggtgggat
      421 tcggattttt gcgcaacagc gccagcgtcg cgagaatcgc agccggagta caaaaaccgc
      481 attggacggc tccgctctcc acaaaagccg tctggatcgg gtgcggttca tgaatcgttc
      541 cgatacctgc aaccgtcaga atatccttct ccattgccga tgcggcgaac acctggcaag
      601 aattgacgag ttttccctcc agaagcaccg agcaggaccc gcacactccc tcgtcgcacc
      661 cgcggtgcac ttccggatga ccatttgcgc gaagcacgtc aagcaatgtt gcatcaggtt
      721 cgaacgtcca ctcattggta cgtccgttca ccaagaaaga ccccttcatc gagacttccc
      781 ctcctcagtc agaacggcct ttaagcctct ttcaacctgt acggcggcaa tctccgaaag
      841 gtattccggt gatcctgccg gttttctccc gaactcaaca gaagccatct tccgaatgtc
      901 aagcgatgca aggacagaaa gatccgctcc cgtaaggcgt tcctcaagct cggtaagacg
      961 gcgcaccctg tccaggcctc cggagactgc aatccggacc ttatctatcg tactgttgtt
     1021 tttgcgtgcg agaatagaga cggtaaaggc cggataatcg aatccgacac gggtctctct
     1081 gtagtaggag gagagccagt cgaccggatc agggaagaga agcgaagtga tcagagagcc
     1141 cttctgaaga gttctgtcgg caagccagga gggcaggtcg taggtcccac cgttcttccc
     1201 ctcggtgcag ataactccgt ggagcaccga catggggccg ataagatcgg accaaagcgg
     1261 aaagagcgac gcgctgcctc caagcgtaat ccggtttctc atcggggtgg aggcagacgc
     1321 tccgagagcc tttcccagga tacattcggg gtgcagattg gcaaggctct ccgcgacgcc
     1381 cccgaacgtc attcccgctc cggcttcaag gattcctttt tccatcttca gcgaatcaag
     1441 gcctaatctg gaaaggtcta ccagaccttc cagggaggcg atcccgcttt tgatcaaact
     1501 cgttcctccg ccgtgaggaa cagttttctc cctgccgaga tacgagatag cttcatcgag
     1561 tgtatccgga aaataccatt gcacagtact catatttctc ctcctttgtt ttgtcatctg
     1621 cacagttcat acactactct ggaggagaga cgttcctcca tccattcact ttataacatt
     1681 caatgtcaac tgtctagagg ggttcttctt aaatttgcag caaagtttcg agagactgtc
     1741 ctgttatgcg ttctattctg cgtgcggttt ttcttgcagc ggcgtatctt tggcgaatag
     1801 ccagcaatgc gttttgatct gcaccatatc gcatacgcat gttctttttc ctggcatcga
     1861 gagaaatgat gtgcgtatcg gaaacgagtt tgtccgcaag atgaagaagg gtagcctcga
     1921 ttcggggcaa agggggcaga tccctgtgac atgctgctat ttctgcgaga agaggaaaac
     1981 cgtgacgccg caaaaaaatc tcgcctgcac acacatgatc cggtacgtgc ctgcacacgt
     2041 cgtgaaacag cgccgcgcca tgaagcagcg gcatatctat cctgactccc tgttgccgca
     2101 aagcgaaagc gagcttcagg caaacagagg cggttgcgtc gcaatgggcg cgaacacgca
     2161 acggcgtgcc cgccaggtcg aagaggacct ctcgttccat tttcgaggga acgcgcagtc
     2221 cgctcaaacg acggtgaatt ttttcaaagt cttgcggcgt atccatatcc atgagcacgc
     2281 cctcgtccgc tacgtcgaag tcttgattcc acgttgccca cccatcaaaa aaggcgcgca
     2341 acccgccttc tccggaaaaa tcaagtatat ggggcgccaa ggcagccgcg ataagcggag
     2401 gatgtccccg cttccctcga aacagagggt gaagaatccc attggaagaa gcgctttcca
     2461 tcattaaacg caatgtggaa acccgaactg tcggaatatc tacgggcagg aggaaaaaag
     2521 cgtctgcact gtgcggaagc gcagctacgc ctacctgtac tgaagtaaac ataccctccg
     2581 cataacgggg attatagacg gcccgggcgc cggcgcgttg agcagctgag gcgacaacgt
     2641 cttcattaaa tcctgtcacg acgagaacat tccgtacccc ggcgccacgc attgaacgaa
     2701 gaatccgctc cagagcagac actccgttca ggagggcaag cggcttgaaa aagcccatac
     2761 ggctggaata ccccgccgcg agaacaaccg ctgcgacttc catcgacaga tctcctttgt
     2821 tgtgttcgct acacactcaa cggcgagcaa acaaggtgta gatcgccgaa tagttccgca
     2881 ggaccgaatt tctataggag gccagcgagc ggccccgata cctgtcgagc cccttatcca
     2941 catctccgcc ggcacgctgg atattcgatg ccagtatatg aacgccgatg cgtatgttgt
     3001 tctcgggctc aaggagctgt ttcgtcgtac ggactggaaa tgtttttcgt atccagggac
     3061 catttgcttt ccagttgacc tgcatcaacc catagttccc cttgctgacg gcattggatt
     3121 taacggtgga ttccttcaca agaatggcgg cgaccagaaa cgggtcaacc ttataatgct
     3181 gggagtattt ttctatcaat ccggcgtaac gggaggcatt tgctgcggaa agacgaggat
     3241 tcactcttct gaagtagctt tcgatcgcgt gcgttctttt tctttgatcc cgagaggtcc
     3301 tgccgctcag catcgcatgg tattctcggc tcgtcgagaa aggcgctgca agggctggtt
     3361 gcgctacaaa acaggagaaa aagaccaagg atatcaggaa acaaaaaacg gtattatttc
     3421 ggattctcat agtgttgccg ctcctttctg cagagaaaaa actgccgcag agggtgaaaa
     3481 agaaaatgca cagtggcatt ttccgcattt ttgggatatt gtagcatata cacatcgaca
     3541 aaaacatttc ttttaaggag gaaattacaa tgaaccttgt tttggagaca ataaagaaac
     3601 gccgtagcgt acgaagtttt cgaccggacc aaattacgga cgacgagctg aatgcaatta
     3661 tcgaaagcgg gttatgggcc ccaagcggac acaacaccca accgtggcac tttcttgttg
     3721 tgcaggacaa atcaaaaatt gaccagatga gccgttccac gatcgaaaag atggcaaact
     3781 cgccgattga ttgggtgcgg aagctggctg cgaaagaagg ataccatctg ttccatggcg
     3841 cgcctacggt catcatcgtt tccggaaggc agtcgggaga tacattcctt cctgtcgtcg
     3901 cggactgcgc agcagctatt cagaacatgt tgctgggcgc agaatccctg aacataggaa
     3961 cctgttggat aggcctgacg agctttctct tcgaggattc tgaagaaacg gcttctttaa
     4021 agataccgga aggctatgct ccgtactact ccattgcagt gggatacaaa agcgccggat
     4081 tcattcccgc tcctcctacc cggaaagagg gaacagtaag cacgttcggc aaataaaaaa
     4141 tggtgcgaga ggggggactt gaacccccac gcccaaaggc actggatcct aagtccagcg
     4201 tgtctgccgt tccaccactc tcgcacgcat gccgtggaat aataccatca tcaaggggcg
     4261 ttgtgaagcc ctgctaaaaa acgaatagcg taaacgaatg ttttattcat tcacactttc
     4321 aaacctgagt ggaaaagagg gaggagacgg ctctgaaaaa aatacggcgt acatcgacat
     4381 tcttctggct gcttttcgga cttgcgacgt ttctcttcat cctctcaaat gcggagtcga
     4441 tcggagacaa tatcagactg ctccttgacg gtgatttctg gcgccaagcg cctcaccgca
     4501 ttccagagaa ggtaatgaat ttcctcaaag agtattcctt tagttttctc tgggcatacg
     4561 gcatctttat tgcgggcatt atctttctcg aagagcgcaa tcccgacagg accatcgcct
     4621 ggctgctcgt cctcctcctg ctgccggtca tcggatttct catctatttt ttcttcggtc
     4681 cggacctcag agaacgagaa aatttccgaa aaataaaaaa gaaacgaaga aaaaacagga
     4741 aaggccccct cttcgtctct catgaacaca acgaaccctg tgccttctta ggggaacaca
     4801 cgaaaaaaac ggcatccttg ct
//
LOCUS       DAOPKU010000193         4821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_19129_length_4821_cov_4.571339, whole genome shotgun sequence.
ACCESSION   DAOPKU010000193 DAOPKU010000000
VERSION     DAOPKU010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19129_length_4821_cov_4.571339"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PK849_07185"
     CDS             complement(<1..189)
                     /locus_tag="PK849_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPE65946.1"
                     /translation="MTKKGKVVLAYSGGLDTSVAVVWLKEQGYDVITMTADVGQQAVD
                     LEAAKSKALRTGAEKAYVL"
     gene            complement(505..1290)
                     /gene="surE"
                     /locus_tag="PK849_07190"
     CDS             complement(505..1290)
                     /gene="surE"
                     /locus_tag="PK849_07190"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPE65947.1"
                     /translation="MKLLITNDDGVFAPGIITLASFLAERGEECTVVAPDRERSSVGH
                     AITLTRPLRLWAIPSGLYSSTCSVYACDGTPSDSVMLGLEEIIPEPDLVLSGINKGPN
                     LGDDLTYSGTVSAAMEALILGRPSLAFSLNCRDADEEQHYETAAAVAGSLLDFLRGSP
                     LPEGVLLNVNVPNVPLPLLKGIKVTRKGVRLYEGKVTRLKDPAGRSYFWVSGRPEDRL
                     EEGSDVWAVAGGFVSVTPVHMDMTHFPSLQTLTANGAEKINFK"
     gene            complement(1306..1905)
                     /locus_tag="PK849_07195"
     CDS             complement(1306..1905)
                     /locus_tag="PK849_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPE65948.1"
                     /translation="MPGIADLLLSVPAVLWAITFHEFCHGYMAYRLGDPTAERAGRLT
                     LNPLRHLDPVGALMLLVFRFGWAKPVPVDPRYFKNPRRDMLLVSIAGVSGNFFTAFVF
                     GMLARFFPYVFLANAALRQFVVLMILINLGLAVFNLIPIPPLDGSKLLYPLLPSFMLE
                     KYFWLERYGFFVLMILMALGVVQAIMGPIVMFFFRLFLF"
     gene            complement(2029..4638)
                     /locus_tag="PK849_07200"
     CDS             complement(2029..4638)
                     /locus_tag="PK849_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPE65949.1"
                     /translation="MKVITSHMGNDFDSLASMVAAQKLYPDAKLCLSGSAGRTVREFL
                     KKNASHWTVLTPRKIKFDEITMLIVVDSRSRSRIGPFASLLGKKSIAVHVYDHHPPSN
                     DDIDGEFLAIEPVGACTTLMVEILLEKRIPIAPHEATLFATGIYEDTGGLTFSGVTAR
                     DFAAVSRLKEIGGDIASIPSFIEMTFSAPERRILDALVENAWVRFINGAKAVFSAVSA
                     PSYVDGLSLFVHRLRDYFDADIAIAAVKMDTRTYLVGRSSEDALDMAAFLTPFGGGGH
                     PQAASVTLHNAKPLPLVREMEQLLHDAVKPSLTAGDIMTSPVMAVPPDSLVDDAYRTM
                     LRYGHSALPVVKGKNILGLITRKDLDKAHLHGFGKTLIREFMTEGVITVPCDASIREA
                     HRLLATHSIGRLPVVDGRRIMGIITRTDLVKALYPLSLPKEERETTPELPWMEDVTTL
                     LDEQLPPEQNKILALLGERAEKLGMRAYIVGGIVRDLLLRRENLDLDIVVEGDAIRFL
                     KSFEKDGFRVSVHERYKTGTLSFPRGVKVDVATARREFYEFPVAQPKVFSDSLKHDLY
                     RRDYTVNAMAISINQSTRGVLVDYFGGRMDLRRKKLKVLHNLSFVEDPTRVIRGVRLE
                     QRLGLTMEDNTLRLIRSCIRGGLLVRLSGFRLRSELELSFREKFPWAAARRMGELGVW
                     DVLFPGIRIDESVRRTFRRLGAFIARISRDFPDFKGRQWLAFFSALLMESSENIRISA
                     LDRLNLSESERGIVVKCLSGLGAAEHTLGGRSSPLNSEIAAFLDGHDPLEAFFWSAAT
                     ERWRVRRRILQYLTRLHRVRPILSGGDLLQLGYAATPRIGVILEKLRILRLDSVVQTR
                     EEEEEYVRKHFPL"
     gene            complement(4635..>4821)
                     /locus_tag="PK849_07205"
     CDS             complement(4635..>4821)
                     /locus_tag="PK849_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65950.1"
                     /translation="TPAETFDRNEKGWFFTFFTLEQWFSLLSQPHCMDSARGPYIERI
                     RRIPWSRSLTLLEELRP"
BASE COUNT         1155 a         1351 c         1274 g         1041 t
ORIGIN      
        1 aagaacatag gctttttcgg cgccagtacg aagagccttg ctcttcgcgg cctcaagatc
       61 aacggcttgc tgccctacat cggccgtcat cgtgataacg tcatacccct gctccttgag
      121 ccataccacc gccactgaag tatccaaacc gccgctgtat gcaaggacaa ctttcccttt
      181 ttttgtcatt tttatccctc caaaagaata gtcgaactaa ataaataaag actcgcccca
      241 atttaccagg gacgagtctt gaacccgcgg taccaccctt tttgacggaa tttccgccct
      301 ctctgtccgt ttctacttgg ccgaggcctt tcttcgaaac cggctccaag gctctcttcc
      361 tccgtgacct gccggaaggg ctctcagccg tggcccgtcc tctctgttgc tgtgctgcgg
      421 agtactcttc ctcatcatcg ccaatacgct tgagtaggga tattataccg actgcgaaac
      481 atttgtcaag taagttgaaa tcactcactt gaagtttatt ttttctgctc cattcgccgt
      541 cagcgtctgt aaagagggga agtgcgtcat gtccatatgc accggggtta ctgagacgaa
      601 tccgccggca accgcccaaa cgtcgctccc ctcttccagc ctgtcctccg gtcttccgga
      661 gacccagaaa taggagcgcc ctgcggggtc cttcaggcgc gttaccttgc cctcgtaaag
      721 acgcactccc ttccgcgtca cttttatccc cttcaacaga ggaagcggta cattagggac
      781 gtttacgttg agcagcaccc cttcggggag cggagaaccc cgcaaaaaat cgagaagcga
      841 tccggcgaca gcggcggctg tttcgtaatg ttgctcctcg tctgcgtccc tgcaattcag
      901 agagaatgca agcgaagggc gtccaagaat cagcgcctcc atcgccgcgg aaacagtgcc
      961 ggaataggtg agatcatcgc ccaggtttgg gcctttattg atgccggaaa gcacgagatc
     1021 cggttctgga atgatttcct ccaatcccag catgacgctg tcggaggggg tcccatcaca
     1081 cgcatacact gaacacgtag aggaatagag ccccgatggg attgcccata agcggagagg
     1141 gcgagtgaga gtaatagcgt ggccgacgct gcttcgttcc ctgtccggag ctaccacagt
     1201 gcactcttca ccccgttccg ccagaaaaga ggcgagggtg attattccgg gtgcaaaaac
     1261 gccgtcgtcg ttcgttataa gaagtttcat gcgattcgac ttctcctaga aaagaaagag
     1321 acggaagaaa aacatcacga tcggtcccat aattgcctga acaactccga gagccataag
     1381 tatcatcagg acaaagaagc cgtaacgctc cagccagaag tatttttcga gcatgaagga
     1441 gggcagcaac ggataaagca gcttcgagcc gtcgagagga ggaatcggga tgaggttgaa
     1501 caccgccaag ccgaggttta tcaaaatcat cagaaccaca aattgcctga gggcagcgtt
     1561 tgccagaaaa acatacggga aaaagcgcgc gagcatgcca aaaacgaagg ctgtaaaaaa
     1621 gtttccggaa accccggcga tggagaccag aagcatgtct ctcctgggat ttttaaaata
     1681 cctcggatct actggaaccg gcttcgccca tccgaaccgg aagacaagca acattaacgc
     1741 tcctaccggg tcaagatgac ggagcggatt cagcgtcaaa cgtcccgccc tctccgcagt
     1801 cggatcgccg agtcggtacg ccatataccc atggcagaac tcatgaaatg taattgccca
     1861 aaggacagct ggaacactga gaagcagatc tgcaatacca ggcattcgta acaatagagt
     1921 cactctcctt aacttgttgt aacgtttttt gccgcacctc gccgttcaga acgtgctcct
     1981 ccgtctcatc ggcaaaccgt agggaagagg gggcccgcgt gctgcgcgtt acagcgggaa
     2041 gtgttttctg acgtactctt cctcttcttc cctggtttgc actacgctgt ccaggcgcag
     2101 gatccgcaat ttttcgagga tgacgccgat gcggggggtt gcggcatacc ccaactgcag
     2161 aagatcacct cctgaaagaa tcgggcggac tctgtgaagg cgcgtaaggt actgaaggat
     2221 tctcctgcgg actcgccagc gctcagtcgc tgcgctccaa aagaacgctt ctaatggatc
     2281 gtgcccgtca agaaatgcgg caatctcgga gttgagcggg ctgctcctac ctcccagcgt
     2341 gtgctcagcg gcgcccaacc ccgaaaggca ttttactaca attccgcgtt ccgattcaga
     2401 gagattcaac cgatccagcg ccgaaatacg tatattttcg gaggactcca taaggagtgc
     2461 ggaaaaaaac gcaagccatt ggcgtccctt gaaatccggg aaatccctgg atattctggc
     2521 aatgaaggcg ccgagcctgc ggaaagtcct gcggacgctc tcgtcaattc gaatacccgg
     2581 aaacagaacg tcccaaaccc ccagctcccc cattctccgc gcagcagccc agggaaactt
     2641 ttcccggaat gagagctcca gctcgctccg gaggcgaaac cctgaaagac ggaccagaag
     2701 tccgcctcgg atgcagcttc gtatcagccg aagcgtattg tcctccatgg tcagtccgag
     2761 gcgctgttcc agccgcacgc cccggataac tcgcgtcgga tcttcaacga agctcaggtt
     2821 atggagtacc ttcagttttt ttctgcgaag atccatgcgg cccccgaaat aatcaaccaa
     2881 aactcctcgc gttgattgat tgatggagat cgccatggca ttcaccgtat aatctctccg
     2941 gtacaggtcg tgtttcagag agtcgctgaa gactttgggc tgggctacag gaaactcgta
     3001 gaattctctg cgtgcggtgg caacatccac cttgacgccg cggggaaatg aaagagtacc
     3061 cgttttgtat cgttcgtgaa ccgagactct gaaaccgtct ttttcaaagc tcttgaggaa
     3121 acgtatcgca tctccttcga cgacgatatc aagatcaagg ttttctctcc gcagaagaag
     3181 atcgcggaca atccctccaa cgatataggc acgcataccc agcttctccg cccgctcccc
     3241 cagaagcgca agaatcttgt tctgctccgg cggaagctgt tcgtccagaa gcgtcgtgac
     3301 gtcctccatc catgggagct ccggagtcgt ctcccgttct tccttgggga gggagagggg
     3361 gtacagggcc ttgacgagat ccgtccgtgt gataatgccc ataatccgtc gaccgtccac
     3421 tacaggaagc ctgccgatac tatgcgtcgc caagagacga tgggcctcgc ggatagaagc
     3481 gtcgcacgga acggtgataa ctccctccgt catgaactcg cgaatcagcg tcttgccgaa
     3541 accgtgaagg tgcgccttat caagatcctt acgggttatc aaacctaaga tatttttccc
     3601 cttgactacg gggagcgcgg aatggccgta gcggagcatt gtcctgtagg cgtcgtccac
     3661 aagagaatcc ggaggcaccg ccataaccgg cgaggtcatg atgtctcctg cggtgagcga
     3721 tggtttgaca gcgtcgtgca aaagctgttc catctctctg acaagcggca gcggctttgc
     3781 gttatgcagc gtcacggatg cagcctgagg atgtcctcct cccccgaaag gcgtgagaaa
     3841 agccgccatg tcgagagcat cctccgagct tcgtccgaca aggtatgtcc tggtatccat
     3901 cttgaccgcg gcaatggcaa tgtcggcgtc gaagtagtcc ctgagacggt ggacgaagag
     3961 cgagagtccg tccacgtacg atggagcgga aacggctgag aaaacagcct tcgctccgtt
     4021 aatgaagcgt acccaggcat tttcaacaag ggcgtcgaga atgcgccgtt ccggggcgga
     4081 aaacgtcatt tctatgaagg agggaatgga agcgatgtcg cctcctatct ccttcaggcg
     4141 ggaaacggcg gcaaaatccc tcgctgtgac gccgctgaag gtcaatccgc ccgtatcctc
     4201 gtagatcccc gtagcaaaga gcgtggcttc atgaggagcg atcggaatgc gtttttccaa
     4261 aagaatctcc accatgagcg ttgtgcacgc gccgacgggt tcaatggcga gaaattcgcc
     4321 gtcgatgtcg tcgtttgatg gggggtgatg gtcatagacg tggactgcaa tacttttttt
     4381 cccgaggagc gaggcaaaag gaccgattcg ggaacgtgac ctggagtcca cgacaatgag
     4441 catggtgatt tcgtcaaact tgatctttct gggcgtcagc acggtccaat gtgaagcgtt
     4501 ctttttcaga aactcccgca cagtccggcc cgcggagcct gaaagacaca gctttgcgtc
     4561 aggatagagc ttctgcgccg ccaccatgct cgcgagggag tcgaaatcgt tgcccatgtg
     4621 gctcgttatg accttcacgg acgaagttcc tccaataatg taagacttcg ggaccatggt
     4681 atacggcgta tccgttcgat gtacggtccc cgggctgaat ccatgcagtg cggctgcgaa
     4741 agaagcgaaa accactgttc aagcgtaaag aaggtaaaaa accacccttt ttcattccgg
     4801 tcgaatgtct cggcgggagt g
//
LOCUS       DAOPKU010000194         4779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_19357_length_4779_cov_4.399873, whole genome shotgun sequence.
ACCESSION   DAOPKU010000194 DAOPKU010000000
VERSION     DAOPKU010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19357_length_4779_cov_4.399873"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1694)
                     /locus_tag="PK849_07210"
     CDS             complement(81..1694)
                     /locus_tag="PK849_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HPE65951.1"
                     /translation="MNEQNRYARSFGEQCTPRALVTGALGAVVITASSMYVALRMGAL
                     PWPTIFVAILSLTVLKALGRTNLNEINVTHTAMSAGGMVAGGLAFTLPGLWMLDGNAT
                     VDTLPLIAVTVSGALLGVVFTALIRRHFVEVEQLPFPMGIAASETVLAGDSGGGKAKL
                     LFSTLGATAVFTALRDWLGWIPGAWMSAGLMAKNIFFGVWIAPMAVGIGYIIGPLYTG
                     VWFLGALLAYFGIIPLGLSFGFFESVDAANAFKNSLGIGLMVGTGLGILVKGVLPRAR
                     EIYGSLFRAESGGVSRLLLPTLLAFVALLLTFGAKMPAVPSLLTILGVWLTTVMAASI
                     TGQTGIDPMEIFGIIVLLAIRLLIPLGMHEAFFVAAVVAIASGVAGDVLNDFKAGYLL
                     KTDPRAQMYSELVGAVVGAFVSVAVLMLMVKAFGKMGPGTELPAPQAYAVSTMVGGLP
                     NPTAFFVGLLIGAALYLVNVPGMTLGIGVYLPMAISTTVFLGGLASFLFRRASASAKE
                     RGVIVASGMLGGEGVTGVLIALIKVLTMS"
     gene            complement(1732..2916)
                     /locus_tag="PK849_07215"
     CDS             complement(1732..2916)
                     /locus_tag="PK849_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE65952.1"
                     /translation="MEHILEEAKRISAEITEWRRHLHQTPELGLETPKTSAYIVQELK
                     KMGVEEIRERVGGWGVAALVKGEKPGKTLAIRADCDALPIKEETGLPFASKNGLMHAC
                     GHDAHTAMALGAAKLLLAHKAELAGTVKFIFQPAEENVQGAKAMIDDGVMENPHVDAL
                     IGLHTGCLWKEIGPGEIGVRFGSLMASVDRFVVTFKGKGGHGATPHLTVDPVTMTATA
                     VLELQTILSRELSPLDSAVLTIGRIEGGRAFNIIAENCVIEGTVRTLNPATRAFVEER
                     LRVVVSRVAEGMRGVAEVEYESGPPALINNADFTRKFHAFAAELAGAEKVKEISEPTM
                     GGEDVAYFLEMVPGTFFVHGACSPEKGQVYPHHNPKFDVDEETFPLGTALFAGFALSW
                     QQ"
     gene            3263..>4779
                     /locus_tag="PK849_07220"
     CDS             3263..>4779
                     /locus_tag="PK849_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65953.1"
                     /translation="MMHIFFVHKKALIACLTILSLLALMPSTGAASAPAPNTFLSSPL
                     YGITHFDSSQSDSTPYGPPRGTFYIESSLQPISYGGPVNIITLASTDPEYMWAVGHDK
                     VSYVHRVSGEWKVVASFEALADASNGLLPAVPDEKFREFGMRNAIGMTVAQMDTYLTE
                     ILGENYKARFGNGAYSVVDKDNVLYTNFLSSLYAFGLKNPANPSEGIEVKRKIADVLK
                     DIQGVEGGESRPAVAPGPTRLFGLSMTYDGFLVVTFSNGVAVIQRDFDLASKVFYPFG
                     EDEYVSNSIAVDENNGMYIASNKLMRKLVWTKEKAISDKEEDGAWATPYDVADVLPPI
                     IKFDNGTGSTPTLMGFGDDSDKLVVITDGAKHMKLTAFWRDAIPEGFVQKPSAASRRI
                     AGQIQATFGLPDPLPEWLQSEQSVVVSGYGAFVVNNMPATVDPDLQATNKICQVALMG
                     PAYPGPLGVERFEWDPATNEWSSVWNRPDVCSTSMVPVHSQSGSMALVNGYYTETG"
BASE COUNT          917 a         1520 c         1437 g          905 t
ORIGIN      
        1 cttcggtatt ctgcgcgtgt ccggtccgca ggccgggaat gtcaaaaaag gacaaggttt
       61 ccatacgctc tccccttccg ttacgacatg gtgagcactt tgatcagcgc gatcaagacg
      121 cctgtcacgc cctcgccgcc gagcatcccc gaggcgacga tgacgccccg ctccttcgcg
      181 gaggccgagg cgcgccggaa gaggaagctc gcaaggcctc ccaggaagac cgtcgtggag
      241 atggccatcg ggaggtagac gccgatgccc agagtcatcc ccggcacgtt gaccaggtag
      301 agcgcggccc cgatcaggag gccgacgaag aaggccgttg ggttcggcag cccgcccacc
      361 atcgtcgaga cggcgtaggc ctgcggcgcg ggaagctccg tccccgggcc cattttcccg
      421 aacgccttga ccatgagcat gagcaccgca acggagacga acgcgccgac caccgcgccg
      481 accagctcgg agtacatctg cgccctcggg tcggtcttca gcagataacc cgccttgaag
      541 tcgttgagga cgtcgccggc cacgccggac gctatcgcga ccactgcggc gacgaagaag
      601 gcttcgtgca tcccgagcgg gatgaggagc ctgatcgcga ggaggacaat gatgccgaag
      661 atctccatcg ggtcgattcc ggtctgcccc gtgatcgagg cggccatcac ggtggtcagc
      721 caaactccga ggatggtgag cagcgagggg acggcgggca tcttcgcgcc gaaggtgagg
      781 agcagcgcga cgaaggccag taacgtcggg agcagcagac gggagacgcc gccgctctcg
      841 gcccggaaga gcgacccgta gatctcgcgc gctcggggaa ggacgccctt cacgagtata
      901 ccgagccccg ttccgaccat caaaccgatg ccgaggctgt tcttgaacgc gttcgccgcg
      961 tccacgctct cgaagaagcc gaaggagagt cccagcggga tgatgccgaa gtaggccagc
     1021 agcgccccga ggaaccagac tcccgtgtat agcggcccga tgatgtagcc gatgccgacg
     1081 gccatcgggg cgatccagac gccgaagaag atgttcttcg ccatcagccc ggccgacatc
     1141 caggcccccg gaatccagcc gagccagtcg cgcaaggcgg tgaacaccgc cgtggccccc
     1201 agcgtcgaga agaggagctt cgccttgccg ccgcccgagt cgcccgcgag gaccgtctcg
     1261 gaggcggcga tgcccatcgg gaacggaagc tgctccactt cgacgaagtg ccgccggata
     1321 agcgccgtga agacgacgcc gagcagcgcg cctgagacgg tgaccgcaat caacggaagc
     1381 gtgtcgaccg tggcgttccc gtcgagcatc cagaggccgg ggagcgtgaa agccagcccc
     1441 ccagccacca ttccgcccgc ggacatggcg gtatgcgtca cgttgatttc gttcagattc
     1501 gttctcccga gcgccttcag gacggtcagt gagaggatgg cgacgaagat cgtcggccac
     1561 gggagcgccc ccatccgcaa cgccacgtac atcgagctcg ccgtgatgac aactgctcca
     1621 agggcgcccg tgacaagtgc gcgcggcgtg cactgttcgc cgaaactgcg cgcgtaccgg
     1681 ttctgttcgt tcatacgatt ccctcccaac aagtgaagtg cttttttctg cctactgctg
     1741 ccagctaagc gcgaatcctg cgaagagggc ggttcccagc gggaaggtct cctcgtcgac
     1801 gtcgaatttc gggttatggt gcgggtacac ctgtcccttc tccgggctgc atgcgccgtg
     1861 aacgaagaag gttccgggga ccatttcaag gaagtaggcc acatcttcgc cgcccatcgt
     1921 cggctcgctg atctccttca ccttttccgc tccggcaagc tccgccgcga aggcgtggaa
     1981 tttgcgtgtg aagtcggcat tgttgatgag agcgggagga ccagactcgt actccacttc
     2041 cgccaccccg cgcatcccct cggcgacgcg cgaaacgact acgcgaagcc gttcttctac
     2101 gaaggcgcga gtggcgggat tcagcgtccg gacggtaccc tcgatgacgc agttctccgc
     2161 gatgatgttg aacgccctgc ccccctcgat tcgtccgatg gtgagcacgg ccgagtcgag
     2221 cggagagagt tcgcggctca ggatcgtctg cagctccagc accgccgtgg cggtcatcgt
     2281 cacggggtcg acggtgaggt gcggcgtcgc gccgtgtccc cccttccctt tgaaggtgac
     2341 gacgaagcgg tcgaccgacg ccatcagcga gccgaatcgg acgccgatct cccccgggcc
     2401 gatctctttc cacagacagc cggtatgcag gccgataagg gcgtccacgt gcggattctc
     2461 catcacgccg tcgtcgatca tcgccttggc cccctggacg ttctcctcgg cgggctggaa
     2521 gatgaacttc accgtacccg ccagctcggc cttgtgcgcc agcagcagct tcgccgcgcc
     2581 aagcgccatt gcggtgtgcg cgtcgtgccc gcaggcgtgc atgaggccgt tcttcgaggc
     2641 aaacggtagt cccgtttcct ccttgatagg caaggcgtcg cagtcggcgc ggatcgccag
     2701 cgtctttccc ggtttctctc ccttcacgag cgccgcgacg ccccatccgc cgacgcgttc
     2761 gcggatctcc tccactccca tcttcttcag ttcctggacg atgtaggccg acgtcttcgg
     2821 cgtctccagc ccaagctccg gcgtctggtg cagatgccgc cgccactccg tgatctccgc
     2881 ggagatcctc tttgcttctt caagaatgtg ctccattcct ccccctccgt tcgtgcagga
     2941 tgattttcgc tccattctac atcattgaga ttcttttctc ttctgtcgaa agagcgtgca
     3001 tcactcgctc tttttgcatc cgttaaaatg tgcgataatc tggtgtatct actccctttc
     3061 gtgcagcgaa cccgttttca cccgtacgga aggggttctt cgacgaaagg agggtgcgaa
     3121 cgggcgcggt gtttttgaaa agcgcacagt gccggctctc tgaatccgct tcttcggaac
     3181 gttttcggga ggatctgcgt cgcttcggat gaatgaaccc gggatactcg atgttcatca
     3241 gaatttatta tcaggaggtt cgatgatgca catatttttt gttcataaga aagcattgat
     3301 tgcgtgcctg acaatactct ctcttctcgc gctcatgcct tcaaccgggg cggcgagcgc
     3361 ccccgccccc aatacgtttc tgtcgtcgcc cctgtacggc attactcatt tcgattcttc
     3421 ccagagcgac tccacgccct acggccctcc ccgcggcacg ttctacatcg agtcgtcgtt
     3481 acagcccatc tcttacggcg gaccggtgaa catcattacg ctcgcgtcca ccgatccgga
     3541 gtacatgtgg gctgtcgggc acgacaaagt gagctacgtc cacagggtga gcggagaatg
     3601 gaaggtcgtc gcctcgttcg aggcccttgc cgacgcgtcg aacggcctct tgcccgcagt
     3661 tccggacgag aaattcaggg aattcggcat gaggaacgca atcggaatga ccgtggccca
     3721 aatggacaca taccttacgg aaattcttgg ggagaactac aaggcgcgct tcggtaacgg
     3781 agcctactcg gttgtcgaca aggacaacgt actctacacg aacttcttgt ccagcctcta
     3841 tgctttcggt ttgaagaatc cggcgaaccc ctccgagggt atcgaagtga agcgcaaaat
     3901 agcggatgtg ctcaaggata tccaaggtgt tgaagggggc gaaagcagac cggcggttgc
     3961 tcccgggcca acgcgcctct tcggtctctc catgacctac gacgggtttc tcgtggtcac
     4021 cttcagcaac ggcgtggctg tcatacaacg cgatttcgac ctcgcctcca aggttttcta
     4081 ccccttcggg gaggacgagt atgtgagcaa ctccatcgct gtggacgaga acaacgggat
     4141 gtacatcgcg agcaacaagc tgatgcgcaa gctggtctgg acaaaagaga aggctatctc
     4201 cgacaaagag gaggacggcg cgtgggctac cccctacgac gtggccgacg ttttgccgcc
     4261 gatcatcaag ttcgacaacg gcacgggctc cacgccgacg ctgatgggat tcggcgacga
     4321 ctccgacaaa ctggtggtca tcaccgacgg cgcaaaacac atgaaactga cggcattctg
     4381 gcgcgacgca atccccgaag ggttcgtcca gaagcctagc gcggcctccc ggcgcatcgc
     4441 ggggcagatt caggcgacgt tcggcctgcc ggatccgctt cccgagtggc tccagtcgga
     4501 gcagtccgtg gtggtctccg gctacggggc cttcgtcgtg aacaacatgc cggcgacggt
     4561 cgacccggat cttcaagcga cgaacaagat ctgccaggtc gctctcatgg gaccggcgta
     4621 tcccggacct ctcggcgtgg agcgcttcga gtgggatcct gcgaccaacg agtggagttc
     4681 cgtctggaac cgtccggatg tttgctcgac cagcatggtg cctgtgcata gccagtcagg
     4741 ctcaatggct ctggtcaacg ggtactacac ggaaaccgg
//
LOCUS       DAOPKU010000195         4771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_19405_length_4771_cov_5.779050, whole genome shotgun sequence.
ACCESSION   DAOPKU010000195 DAOPKU010000000
VERSION     DAOPKU010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19405_length_4771_cov_5.779050"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PK849_07225"
     CDS             <1..343
                     /locus_tag="PK849_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HPE65954.1"
                     /translation="DAAAGARAVKGYVIPERGVQFEIKPGQSLPGGMEPPDFYVEGEG
                     LMTWRNVVEDNGPFDHNRAAGMVTFRAVSIRAEAQTAPTGGSGCSSATASPFALLLGV
                     PLLLLRKTRRI"
     gene            613..2181
                     /locus_tag="PK849_07230"
     CDS             613..2181
                     /locus_tag="PK849_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPE65955.1"
                     /translation="MALLLCPASQAAAVAMWNVTSGADDPSNPAVGTFRYTALHASPG
                     DTVRFTGAGTTVVLADQVLIDRTLTLNGPATIRQGAVNKRVLEVTATCTMNNITITGG
                     NFENQGLSALKGAGIYNRTTGVLTMNSCTLTGNMRGGAIENYGNLTMNSCTLTNNTNS
                     LAFGSAVTNAYLGGGLHASAALNGCTIENNDVAGSLGGAIYNGDILVMSNTKVCNNTT
                     GKGSGLQSRKGGGLYVGSRSNTTLTNGCRVTGNDPSQLYKEETYFSTPVFTYDSTCVI
                     GDAPNKSATAFSGYTGETAPEPRSIKDDADVAEVKRKLADSGSDLFATVSRALSSDLG
                     RTPDGTSATLAGLTASLYYANTFENVGVESQDLVVEYTASWPENARYYALFARADNAG
                     YELAERGIQFELKAGQSLPDGATPPDFYVPGEGLMTWRNVVTDGGPYDLNPAAGVVTF
                     RVCSIRAAEATGDTGSGGCNASGGFAPLALLLLVPVQAFLFRRSCSGGEECRNRGNGF
                     IRGTKTRFSNTEGR"
     gene            2188..4011
                     /locus_tag="PK849_07235"
     CDS             2188..4011
                     /locus_tag="PK849_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPE65956.1"
                     /translation="MRQTKSHVFFMLLLLFALMPTFFPGASRAFGDTASNGAATVLEV
                     VDTSDDPSNPAPGTLRYLALHASDGDIIRFAEGKNDISLKATLEIEKSIAILGPATLT
                     QTGSGAVVILKAQDKEATLTNLTFSGGTDKNMSYAGSGVINWGTMHLSYCTMTGNRTY
                     WQGAGVNNTKTLTMTNCTVKGNFSNTGGGGVYSRGTLVIQDSVVEDNLCYDDFGGGIA
                     NEGTLTVKRCVVRNNTANSPLSQSDIILGTNPGYGGGIGSLSGAVAIYDSSISGNQGH
                     FGGGIYGKNGSITLQDSSVTGNTAGAFGGGIYCFKGSVTLQTSRITDNSLTADYYGGG
                     LCLSSSTASLLIETVISGNAPDQIWSPAPSSWTSDGTCMVGDAPNRSATAFSGYSGDA
                     EPEPRSIIGDADVAEVKTALANPESALYGAIEQALAADLGKSAAEKSAALVRLSGMAA
                     TLYYANTFENVALTSTDLSVEYTASWPENVRYYSLFSKADGSGYENPGRGIQFEIKAG
                     QSLPDGATPPDFYEEGEGLMTWRNVVTDGGSFDLEPAPGIVTFRVCSVRAAEAPAASS
                     GGSGCSTDVASPLALLLAIPLLLLRKMQRKTHRTSQGGDQR"
     gene            4008..4163
                     /locus_tag="PK849_07240"
     CDS             4008..4163
                     /locus_tag="PK849_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65957.1"
                     /translation="MTQERKKAEAKLEDNLKPEELKKKKKLGEQDDAELDGISGGEGM
                     DTGKIIP"
     gene            4319..>4771
                     /locus_tag="PK849_07245"
     CDS             4319..>4771
                     /locus_tag="PK849_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE65958.1"
                     /translation="MELQLIRNATVKMSFAGKMLLLDPYLGEKGSGPSYTGKEHSPVV
                     SLPIALEEVLDGVDAVVVSHVHSDHFDTTAREILPKDLPLLCQPEDEQTMRDLGFTDV
                     HPVAGTLWLDEISVSRTSGRHGSGAVLKDMGVVSGFFFEAPGEPSVYWA"
BASE COUNT          970 a         1442 c         1542 g          817 t
ORIGIN      
        1 ggacgccgct gcgggagccc gcgccgtgaa ggggtacgtc atccccgagc gcggcgtgca
       61 gttcgagatc aaaccggggc aaagccttcc cggcggcatg gagcctcccg acttctacgt
      121 ggaaggcgaa gggctgatga cgtggcggaa cgtcgtggag gacaacggac ctttcgacca
      181 caaccgcgct gcgggcatgg tgacgttccg cgcggtctcc atccgcgccg aggcccagac
      241 ggctcccaca ggcggctccg gctgctcgtc agcgaccgcg tcgcccttcg cgctcttgtt
      301 gggagtcccg ctgctccttc tgcggaaaac acggcgaatc tgaaggcgcg ctataaccga
      361 cgctcgccgg aagcgaccga cggcacggga agcggcgccg cgagagaatt ctccggcgcc
      421 atccgcgcct cctagaaggc ggaaacgcgt acaaccagcc ttccgcttcc ttatgtctat
      481 aaacgaattc aaattttctt gactttacgg cgcatgcttt tttcgcagaa cgcgaatact
      541 caacgaaagg aagcgaagac tatggtgcaa cgaggttgtt tcggttttcg ggctttgagg
      601 atgataacgg tgatggcatt actgctctgc cctgcgtcgc aggcggcggc cgtcgcgatg
      661 tggaacgtga cgagcggggc ggacgacccg tcgaatcccg ctgtgggaac cttccggtac
      721 acggcgctcc acgcttcgcc gggggacacc gtccggttta ccggcgcagg aacgacggtc
      781 gttctggcgg accaggtgct catcgacagg acgctcacgc tcaacggccc cgcgacgatc
      841 cggcagggag ctgtgaacaa gcgcgtcctc gaagtgacgg cgacgtgcac gatgaacaac
      901 atcacgatca cgggaggcaa cttcgagaac caggggttgt cggcgctgaa aggggccggg
      961 atctacaacc gcacgacggg ggtcctgacg atgaactcgt gcacgctgac gggcaacatg
     1021 cgcggcggcg ccatcgagaa ctacggaaat ttgacgatga actcgtgcac gctgacgaac
     1081 aacacgaact ctttggcgtt cgggtccgcc gtcacgaacg cgtaccttgg cggcggcctc
     1141 catgcgagcg ccgcgctgaa cggctgcacg atcgaaaaca acgacgtcgc ggggagcctc
     1201 ggcggcgcga tctacaacgg ggacatcctc gtcatgagca acaccaaagt ttgcaacaac
     1261 acgaccggaa agggcagcgg gctgcagagc cgcaagggag gaggactcta cgtgggttct
     1321 cgctcgaaca caacgctcac gaacggatgc cgagtgaccg gaaacgatcc gtcgcagctc
     1381 tataaggagg agacgtactt ctcgacgccg gtcttcacgt acgacagcac gtgcgtgatc
     1441 ggcgacgcgc cgaacaagtc ggcgacggcg ttctcgggat acacgggaga aacggcgccg
     1501 gaaccgcgct cgatcaagga cgacgccgat gtcgccgaag tgaagcgcaa actggccgat
     1561 tccggaagcg atctcttcgc gaccgtcagc cgggccctct cttcggacct cggcagaact
     1621 cccgacggaa cgagcgcgac gctcgcgggt ctgaccgcgt ccctctacta cgcgaacacg
     1681 ttcgagaacg tgggcgtcga gagtcaggat ctcgttgtcg agtacacggc gtcgtggccc
     1741 gagaacgcgc ggtactacgc gctcttcgcc cgcgccgaca atgcggggta cgaacttgcg
     1801 gaacgcggca tccagttcga gctgaaggcc gggcagagcc tgcccgacgg cgcgacgccc
     1861 ccggacttct atgtcccggg cgaggggctc atgacgtggc ggaacgtcgt gacggacggc
     1921 ggaccgtacg acctgaaccc ggcggcgggc gtcgtgacct tccgggtgtg ctcgatccgc
     1981 gccgcggagg ctacgggcga cacaggcagc ggaggttgca acgcttccgg aggcttcgcg
     2041 ccgctcgcgc tgctgcttct tgttcctgtt caggcgttcc tgttccggcg ttcctgttcc
     2101 ggcggcgaag agtgtcgtaa ccgcgggaat ggtttcatcc gcggaacgaa aactcgtttt
     2161 tcaaatacgg aagggagata gcgaagaatg agacaaacga aatcccatgt tttttttatg
     2221 ctgctgttgt tgttcgcact gatgccgacg ttttttcccg gggcgtcccg cgcctttggg
     2281 gatacggctt ccaacggcgc cgcgacggtc ctggaggttg tcgatacctc cgacgacccg
     2341 tcgaatcccg cgccggggac gctccgctat ctggcgcttc atgcctcgga cggcgatatc
     2401 atccgctttg cggagggaaa gaacgacatc tccctcaagg cgacgctcga gatcgaaaag
     2461 agcatcgcca tactaggccc ggcgacgctg acgcagacgg gaagcggcgc cgtcgtgatc
     2521 ctgaaggcgc aggacaagga ggcgacgctc acgaacctga cgttctcggg aggaacggac
     2581 aaaaacatga gctacgccgg ttcgggcgtc atcaactggg ggacgatgca cctgtcgtac
     2641 tgcacgatga ccgggaaccg aacgtactgg cagggagcgg gcgtcaacaa tacgaagacg
     2701 ctcacgatga cgaactgcac cgtgaaggga aatttctcca acaccggcgg agggggcgtc
     2761 tactcccgag ggacgctcgt catacaggac tccgtcgtcg aggacaacct ctgttacgac
     2821 gacttcggcg gcgggatcgc caacgagggg acgctgaccg taaaacgttg cgttgtgcgg
     2881 aacaacacgg ccaactcgcc cttgtcgcaa agcgacatta ttctgggcac gaatcccggg
     2941 tacggcggcg ggatcgggag cctgtccggg gcggtcgcca tatacgactc ttcgatcagc
     3001 ggcaaccaag gccacttcgg cggcgggatt tacggcaaaa acgggagcat caccctccaa
     3061 gactcgtcgg ttacgggcaa tacggccggc gcgttcggcg gcggcatcta ctgcttcaag
     3121 ggaagcgtga cgctccagac gtcgcggata acggacaact ccctgaccgc ggattactac
     3181 ggaggcgggc tctgcctctc gtcgagcacc gcctcgctgc tgatcgagac ggtcatcagc
     3241 ggcaacgccc cggatcagat ctggagtccc gctccgagtt cttggacgag cgacggcacg
     3301 tgcatggtcg gcgacgcgcc gaacaggagc gcgacggcgt tttccggtta ttcgggcgat
     3361 gccgagcccg aacctcgctc gatcatcggc gacgccgacg ttgcggaagt gaaaaccgcc
     3421 ctcgcgaacc cggagagcgc gctgtacggc gcgatcgaac aggcgttggc agcggatctg
     3481 gggaaaagcg ccgccgaaaa gagcgctgcg ctcgttcggc tctccggcat ggcggcgacg
     3541 ctctattacg cgaacacgtt cgagaacgtc gccctgacga gtacggatct ttccgtggag
     3601 tatacggcct cgtggccgga gaacgtacga tactattcac tcttctccaa ggccgacggc
     3661 tccgggtacg agaaccccgg acgcggcatc cagttcgaga tcaaggccgg gcagagcctt
     3721 cccgacggcg cgacgccgcc cgacttctac gaggagggcg aagggctgat gacctggagg
     3781 aacgttgtga cggacggcgg atcgttcgac cttgaaccgg cgcccggaat cgtgaccttc
     3841 cgcgtgtgct cggtccgcgc ggccgaggcg ccggcggcct cctcgggagg atcaggctgc
     3901 tcgacggacg ttgcgtcgcc tttagcgctg cttctggcga taccgctgct tcttctgcgg
     3961 aaaatgcagc ggaaaacaca ccgcacatca caaggagggg atcagcgatg acgcaggaac
     4021 ggaagaaggc ggaagccaaa ctggaggata acctgaagcc ggaggaactc aagaagaaaa
     4081 agaagctcgg cgagcaggac gacgctgaac tggacggaat ctccggcggc gaggggatgg
     4141 atacggggaa gatcataccg tagcgttttt cgaaagctgg aaagagattc gcgatctctt
     4201 tccagctttt ttagggtgcg tttgctccag tcgtccaaag aacctgctcg ttgaaaattt
     4261 ctcttgaccg tccgtctccc tgcggcgtat catcgggcac agaaggaggc ggtatatttt
     4321 ggagctgcaa ctcatccgca atgccaccgt aaaaatgtcg ttcgcaggaa aaatgctgtt
     4381 gctggacccg tacctggggg agaaggggtc ggggccttcc tacaccggga aggagcacag
     4441 cccggtcgtt tcgctgccca tcgctcttga ggaggtgctc gacggggtgg atgccgtcgt
     4501 cgtctcccat gtgcactccg accacttcga cacaacggcc cgggagattc ttccgaaaga
     4561 tcttccgctg ctctgccagc ccgaggacga acagaccatg cgcgacctcg gcttcaccga
     4621 tgtacacccc gtcgccggaa cgctttggct ggacgaaatt tccgtctccc gcacatcggg
     4681 gcgccacggt tcgggcgcgg tgctgaagga catgggcgtc gtctcgggct tcttcttcga
     4741 agcgcccgga gagccgagcg tctactgggc g
//
LOCUS       DAOPKU010000196         4765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_19444_length_4765_cov_4.806582, whole genome shotgun sequence.
ACCESSION   DAOPKU010000196 DAOPKU010000000
VERSION     DAOPKU010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19444_length_4765_cov_4.806582"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PK849_07250"
     CDS             <1..779
                     /locus_tag="PK849_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65959.1"
                     /translation="GGIQLDCVSAGYGERRVLDGLSAEFPSGALTMLLGPNGSGKSTL
                     LRLLGGALPFSGNAVLAGRSLRRLSPGQRGKLVGMVSQSPSLTFPFTVEEVVLLGRLP
                     HRKFLSGWTMEDRGCAMEAACEMELGEMLSRRVSSLSGGEKQRVLIAQVIAQQPEVFL
                     LDEPSSALDPRHTLRLFRFLRKCAREGATVVAAAHDINLAAEYADCVCFLKNGRIEAF
                     GDVEDALNDTVLSSVYDVPFASFDADLREDGRWRRVWRAV"
     gene            752..1738
                     /locus_tag="PK849_07255"
     CDS             752..1738
                     /locus_tag="PK849_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE65960.1"
                     /translation="MEKSMARRVAGIFPLIFVLCMLLFPVPCRAAGITVVDDAGRTVV
                     LAAPAERIVSFYAGHSENILALGGAGRLVGIAEGDDETLFPGVPRLPMRADAERILAL
                     APDLVLVRPLAESVNEGVLRTLERSGVPVVSLSPPIPETMEAYLERLSSLLGAEDGVN
                     LWKETLALLKAQIPPVDAARPLVFLETSAKGLRTCAPGSWAALLLSLAGGVNAAASAV
                     PLRPESPLANWGEERLLALAAEGLDVYIVQTGAMNPVTEADVLARPWIKGVHGAEIVL
                     LPEEDVSRPSLLRMKTSVEMLRAIFERGRRENHELPGAKEPMLSGDEEEDER"
     gene            1735..2427
                     /locus_tag="PK849_07260"
     CDS             1735..2427
                     /locus_tag="PK849_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HPE65961.1"
                     /translation="MKFWGVGLGPGDPELVTLRALRILKEADAVFIPFSEKGRESVAG
                     RILEAHLVRETLPVHFPMIRDDETRDALLCEELARLRPFWENAASVALPVIGDSALYA
                     TAAYLFDALKSMAPDVELGLVPGVSAHSLASSRAGRFLALGSEPLSVIPGTAPLARIE
                     AMLAAADSAVLYKPSALGEELRSLVERSGPWRTILRIDRAGLPDERILEGEEALVPSS
                     EYLSVVQLLRWR"
     gene            2579..3664
                     /locus_tag="PK849_07265"
     CDS             2579..3664
                     /locus_tag="PK849_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65962.1"
                     /translation="MVGATDWRGLLYGELLLVVTVTAGLGFGKLLLAASVPERVMRYL
                     LPGADARRGRGGLHPDLLFALAVSLGSSRAGAALVAEAQREGRLSRREAVFGTLLQSF
                     PGYIKRWLISFPTAFGLAGAAGAIYSLIVLLRSFCRFLFFLALLRKGPSHDLPQGAAS
                     GVSHPPFVPGESATLPGAEPGREDGASAKIRDSILPQQTHEREAVAQGPAGGVHVSRG
                     RALPLFRTLARTLPLACFVYALAYLLSPSIQAFLQEKGASFPLLSSAGWTVAAASFAH
                     VNAALGVAGGAIASGALSTAQGVLALLVGNMLAVLSRVLRQDLAFWLGIFPGRMVRSL
                     FVWNLLTLVVTMAVTVALAAIPVLRGW"
     gene            3693..>4765
                     /locus_tag="PK849_07270"
     CDS             3693..>4765
                     /locus_tag="PK849_07270"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPE65963.1"
                     /translation="MMNSAELFERAKRSLVGGVNSPVRSWRGVGGTPLFFQRGEGPFL
                     HSVDGGRYTDYVCSWGPLILGHAHPEVVEAVCVAARESTSFGAPCPKEVELAEEVRRV
                     FPSMELARFVSSGTEAVMTALRLARGFTGREIVLKFDGCYHGHSDGMLVSAGSGALTL
                     GKPDSGGVPESVASTTLVVPYNDLERVSEAFDRFGGRIAAVIVEPWAGNMGLVPPIPA
                     NGSAEGFLEGLRRLTSAHDALLIFDEVITGFRVPEGGVQQRTGVTPDLTCLGKIIGGG
                     LPVGALGGRREICEHLAPLGPVYQAGTLSGNPLAMASGLAVLRVLRREGVYDMLEERA
                     KQLADGMSGAAANAQTAQPAQDLP"
BASE COUNT          689 a         1475 c         1681 g          920 t
ORIGIN      
        1 gtgggggaat tcagctagat tgcgtgtccg cagggtacgg ggagcgtcgt gtgctcgacg
       61 ggctctccgc cgagtttccg tccggcgccc tgacgatgct gctggggcca aacggcagcg
      121 gcaagagcac gctgttgcgt ctgctggggg gcgcgctccc gttctcaggg aacgcggttc
      181 tggccggccg ttcgcttcga cgcctctctc ccggacagcg gggaaagctt gtcggaatgg
      241 tctcccagtc gccgtcgctc acctttccct tcaccgtcga ggaggttgtg ctcctcggaa
      301 ggctccctca cagaaagttt ctctcaggat ggacgatgga ggacagaggg tgcgcgatgg
      361 aggccgcgtg tgaaatggag ctcggtgaga tgctctcccg cagggtgtcg tcgctctccg
      421 gaggggagaa gcagcgggtg cttatcgcgc aggtgatcgc gcagcagccg gaggtcttcc
      481 ttctcgacga accgtcttct gcgctcgacc cccggcacac gctccggctt ttccgcttcc
      541 tccgaaaatg cgcacgcgag ggggcgacgg tggtcgcggc ggcccacgac atcaatctcg
      601 cggcggagta cgccgactgc gtctgtttct tgaaaaacgg gcgcatagag gccttcggag
      661 acgtggagga tgcgctcaac gatacggttc tgtcgtcggt ctacgacgtt ccctttgcgt
      721 cgttcgacgc ggatctccgc gaggacggcc ggtggagaag agtatggcgc gccgtgtagc
      781 gggaatcttt ccgctcattt ttgtcctctg catgctgctc tttcctgtgc catgccgcgc
      841 cgccgggatt acggtcgttg acgacgccgg gcgaaccgtc gtacttgccg cgccggcgga
      901 gcgaatcgtc tctttctacg ccggacacag cgagaacatt ctcgccctcg ggggggccgg
      961 caggcttgtc ggaatcgcgg agggcgacga cgaaacgctc tttcccggcg tcccccggct
     1021 gccgatgcgg gccgacgcgg agcgaattct tgcgcttgcg ccggatcttg tgctcgtccg
     1081 ccctcttgcc gagagcgtca acgaaggcgt tttaaggacg ctggagcgaa gcggcgtccc
     1141 cgtggtttcg ctctccccgc cgattcctga gacgatggag gcgtaccttg agcgcctctc
     1201 ctcgttgctc ggggcggagg acggtgtgaa tctctggaaa gagacgctcg ctctcctgaa
     1261 ggcgcagatt cctcccgtag atgccgcccg tccgctcgtc ttcctggaga cttcggcaaa
     1321 gggcctccgc acgtgtgcgc ccggctcatg ggcggctctg ctgctctcgc tcgccggagg
     1381 agtgaacgcc gccgcgtccg cggttccgct gcgtcccgag agcccccttg cgaactgggg
     1441 ggaggaacgc ctccttgcgc ttgctgcgga ggggctcgac gtctacatcg ttcagacggg
     1501 ggcgatgaat ccggtcacgg aagcggacgt cctcgctcgc ccgtggatca aaggcgtgca
     1561 cggagcggag atcgtcctgc ttcccgagga ggacgtcagc cgtccgtcgc tgctgcgtat
     1621 gaaaacgtcg gtggagatgc tgcgtgcgat ttttgaacga ggacggagag aaaatcatga
     1681 actgcccgga gcaaaagagc ctatgctctc cggggatgag gaggaagacg aacggtgaaa
     1741 ttctggggag tgggcctcgg cccgggcgat cccgagctgg taacgctccg ggcgctgcgt
     1801 attctgaagg aggcggacgc cgtcttcatt ccgttttcgg agaagggaag ggagagcgtg
     1861 gcgggacgga tactggaagc gcatcttgtt cgcgagacgc tgcccgtcca tttcccgatg
     1921 atccgcgacg acgaaacgcg cgatgcgctg ctgtgcgagg agcttgcgcg gctccggccg
     1981 ttctgggaga acgcggcctc cgtggcgctg cccgtcatcg gcgattccgc cctctacgca
     2041 acggcggcat atcttttcga cgcgctgaag agcatggcgc cggatgtcga actcggcctt
     2101 gtcccgggag tttcggcaca ttcgctggcc tcgtcgcgcg cggggcgttt tttggcgctg
     2161 gggagcgagc cgctctcggt cattccggga accgcgccgc tcgcgcggat agaagcgatg
     2221 ctcgccgctg cggactcggc ggtcctctac aagccgtccg cgctcggaga ggagctgcga
     2281 tcactggtgg agcgcagcgg gccgtggcgg acgatacttc ggatcgacag ggcggggctt
     2341 cccgacgaga ggattttgga gggggaagag gcgctcgttc catcctccga gtatctgagc
     2401 gtggtgcagc ttttgcgatg gcgctgaccc cttgtccgca cgccgcccgc ttcgccgcgg
     2461 ggaacggacg tggccgccgc gacgaggaac gcgcggatag ctgcatccgg gcggcgcaca
     2521 tgcggagctt cctgtgtacg ccgttttcgc ggagacggca gcagagaagg cgtgcgccat
     2581 ggtaggcgcg acggactggc gcggactgct ctacggcgag ctgctgctcg tcgtcaccgt
     2641 gaccgccgga cttgggttcg gcaagctgct cctcgccgct tcggtgccgg agcgcgtgat
     2701 gcgatatctt ttgccgggag cggacgctcg gcgcggacgc ggagggctgc acccggacct
     2761 tctcttcgct ctggcggtga gcctcgggtc gtcgagggcc ggggcggcgc tcgtggcgga
     2821 ggcgcagcgg gagggcaggc tttcgcggcg tgaagcagtc ttcgggacgc tgcttcaatc
     2881 gtttccgggg tacatcaaac gctggctcat ctccttcccc accgcgttcg gcctcgcggg
     2941 agccgccggg gcgatctact cgctcatcgt gctgctgcgc agcttctgcc gttttctctt
     3001 cttcctcgcg cttctgcgga aaggcccgtc tcacgatctg ccgcaaggag cggcgtcggg
     3061 tgtttcgcac ccccccttcg tcccgggtga atctgctacg ctccccgggg cggagccggg
     3121 gagagaggac ggagcctctg caaagatccg ggacagcatt ctcccgcagc agacgcacga
     3181 aagagaagcg gttgctcagg gaccggccgg aggcgtgcac gtcagccggg ggcgcgctct
     3241 tccgctcttc cggacgttgg cgcgcaccct cccgctcgcc tgtttcgtct atgcgctggc
     3301 gtatctgctt tcgccgtcga tccaggcttt tcttcaggag aaaggagcct cctttcccct
     3361 gttgtcgagc gcaggatgga cggtcgccgc cgcgtccttc gcgcacgtca acgcggccct
     3421 cggggttgcg ggaggcgcga ttgcctcggg agcgctctcc acggcgcagg gggtgctcgc
     3481 gctgctcgtc gggaatatgc tcgcggtgct ctccagggtg ctccggcagg atctcgcctt
     3541 ctggctcggg atctttccgg ggcgcatggt ccgctcgctc ttcgtgtgga atctgctcac
     3601 gctcgtcgtc acgatggccg tgactgtcgc attggccgcc atcccggtgc tcagagggtg
     3661 gtagatatgg tgtttcaggg gaggtttttg aaatgatgaa cagcgcggaa ctcttcgagc
     3721 gggcgaaacg ctcgcttgtc ggcggcgtga acagtccggt gcgctcgtgg cgcggagtcg
     3781 gcggaacgcc gctctttttt cagaggggcg aggggccgtt tctccactcc gtcgacggcg
     3841 ggcggtatac ggactacgtg tgcagctggg ggccgctgat cctcggacac gcccatccgg
     3901 aagttgtcga ggctgtctgc gttgccgcgc gggagtcgac ctccttcggc gcgccctgcc
     3961 caaaagaggt cgaacttgcc gaggaggtgc ggcgcgtctt cccctcgatg gagctggcgc
     4021 gcttcgtctc ctccgggacc gaggcggtga tgacggcgct ccgtctggcg cgcggtttca
     4081 ccgggaggga gatcgtgctg aagttcgacg gctgctacca cgggcactcc gacggcatgc
     4141 tggtgagcgc cgggagcggc gcgctcaccc tggggaaacc cgacagcggc ggtgttccgg
     4201 agagcgtggc ctcgacgacg ctcgtcgttc cgtacaacga tcttgagcgg gtgagcgagg
     4261 cgttcgaccg tttcggcggc cgtatcgccg ccgtgatcgt cgaaccgtgg gcagggaaca
     4321 tggggctggt tcctccgata cctgcaaacg gctctgcgga gggttttctg gaggggctgc
     4381 ggaggttgac ctccgcgcac gacgcgctgc tgattttcga cgaggtgatc accggcttcc
     4441 gtgttccgga ggggggagtg cagcaacgaa cgggcgtgac gcccgatctt acctgcctgg
     4501 gaaagatcat cggcggcggt ctgcccgtcg gcgcgctcgg cggcaggcgc gagatctgcg
     4561 agcatcttgc cccgctcggc cccgtctacc aggcggggac gctctctggc aatccgctcg
     4621 cgatggcctc gggactcgca gtcctgcgtg ttctgcggcg agagggcgtc tacgacatgc
     4681 tggaggagcg ggccaagcag cttgcggacg gcatgtcggg tgcggccgcg aacgcccaga
     4741 cagcgcaacc agcgcaagac ctccc
//
LOCUS       DAOPKU010000197         4741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_19588_length_4741_cov_4.504908, whole genome shotgun sequence.
ACCESSION   DAOPKU010000197 DAOPKU010000000
VERSION     DAOPKU010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19588_length_4741_cov_4.504908"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK849_07275"
     CDS             <1..181
                     /locus_tag="PK849_07275"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520379.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPE65964.1"
                     /translation="ESQETLDRFAAAMEEIVAKAKAEGAEPFRDMPLTTPVSRPDETT
                     AARNPILRWQFGDAE"
     gene            265..1620
                     /locus_tag="PK849_07280"
     CDS             265..1620
                     /locus_tag="PK849_07280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPE65965.1"
                     /translation="MYDLIVIGGGPGGLRAAELASHAGMSVALVEKDRLGGTCLNRGC
                     IPTKALYAHIVGGKGERDGLWSRVEATMDKLRQGSATALRMAKVKTYRGTATVTQWEG
                     EKKVSVRKEDGSVEEITSSRLLVAAGARSVRPAFAGNDLPEVLTGDWAIVDPLLWDPE
                     RNQSVRTVAVLGAGVIALEMAMMLQNLGKKVILLKHSDQILRRLDGDVKKKVAQIMKK
                     RGTDVREYVRLSEAVRDGGRLLLRGTAKDEPFEERCDRLLLASSMEPILDGFGLENGP
                     VRVEKGCIAVDESMMTSVEGVYAVGDCTGGAMLAHLAEYQALAAVSHMTGGSYRVDLD
                     ALPACVFIEPEVATVGLTEEEARARGEEIVSAKAHFGANGMALAMGEGDGFVKVVARA
                     ADRTILGVHIMGPEAASLLGETTLAVAKHLTAEDVANTIHAHPTLCECFRDACRRVME
                     E"
     gene            complement(1768..2883)
                     /locus_tag="PK849_07285"
     CDS             complement(1768..2883)
                     /locus_tag="PK849_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE65966.1"
                     /translation="MASEQAESKGKIMIVDDTVVNLELLQNLLQRRGYSIMAFPQGKL
                     ALKAALKSPPDMLLLDINMPEMDGFEVCSHFKSEPSLAKIPVLFISALEASQDKVRAF
                     AAGGVDYVTKPFQIEELNARVDTHLKIVRMQRELERYNHELEELVQEKVRDIEESHLS
                     TILAMVKLTEYRDVGTGMHIERTRTLCSMLAAKLAEHPEYDGLLSPEYAETIYNAAPL
                     HDVGKVGVPDGILLKPGRLTDDEWEIMKTHSEIGAETLREVRRKYVSNAFVNMGIEIA
                     LFHHEKWDGSGYPEGRSGEAIPLSARIMAVADVYDALRTRRPYKEPFSHEESFAILQK
                     DSGSHFDPLLVEVFAQLEDEIKDLYNRCADLDGSSCR"
     gene            complement(2873..>4741)
                     /locus_tag="PK849_07290"
     CDS             complement(2873..>4741)
                     /locus_tag="PK849_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE65967.1"
                     /translation="ERREREDEVRKQAGLIASMLNSIPDIIFYKNTDGVFLGCNRPHE
                     EMTGRSESETVGKTDYDFYDRGIADFYREKDKEALAAERPLHNEEWVTYPDGRRALLD
                     TLKTSFRGKDGELIGLLGISRDITERKRMEEDLRAALEAANAASLAKSAFLASMSHEI
                     RTPMNAILGFSQLLERDPELAPKHMKYVRIINRSGEHLLALINDILDMAKIESGRMTL
                     NRAPFSPFAMLRDIVSLFHTLAEEKSLSLTIEHDDDLRAATLLGDEGKLRQVLVNLVG
                     NGLKFTQQGSVTVRFRTETDFRDASSDERELLLSVEVEDSGVGISPEQIELIFAPFQQ
                     SDSGKKAGGTGLGLTISRKFVEMMGGTLTCESELGKGSCFRFRIPVERVYDVSLRPSQ
                     LGGRVLGLKPESGRPLILIADDDDTSRGLLTELLTEAGFDCAEAANGLQAVELCASRT
                     PDAVLMDMNMPLLDGYEATRMIKESDSRNIPVIALTADAFARDAVAACGADEYLRKPF
                     VADELFAVLQTVLGLEYIYEDVSGRDTAGGEAGSEAGGPGSPKAIDLSLIPGNLLREL
                     HGAVEDGDMYRIRELIRSISAVDEKAGKELLLLAERYDYERLLELVNDEGGLSDGE"
BASE COUNT          841 a         1530 c         1486 g          884 t
ORIGIN      
        1 ggagtcgcag gagacgctcg accggttcgc cgcagccatg gaggagatcg tggcgaaggc
       61 gaaggccgag ggggcggagc cgttccgcga catgcccctg acgacgcccg tctcccgccc
      121 ggacgagacg acggcggcgc ggaatccgat tctccgctgg cagttcgggg acgcggagta
      181 accgcgcagc gacagtatca tcgagggggc gcgccgcgtg tgcgacgccc ccttttcagt
      241 accgcacagg gaaggagaga gagcatgtac gacctgattg tcatcggcgg cggcccggga
      301 gggcttcgcg ccgcggaact ggcgtcgcac gcggggatga gcgtggcgct ggtcgagaag
      361 gaccgcctcg gcggaacctg cctcaacagg gggtgcatcc ccaccaaggc gctctacgcg
      421 catatcgtcg gcggcaaagg tgaacgggac ggactctgga gccgcgtgga ggcgacgatg
      481 gacaagctgc gccagggctc ggcaacggcg ctgcgcatgg cgaaggtgaa gacgtacagg
      541 ggaacggcca cggtaacgca gtgggaggga gagaagaagg tctccgtccg caaggaggac
      601 gggagcgtcg aagagattac ttcgtcccgg ctgctcgtcg ccgcgggggc gcgctccgtc
      661 aggccggcgt tcgcggggaa cgatctgccg gaggtgctga cgggcgactg ggccatcgtc
      721 gacccgctcc tctgggaccc ggagcgcaat cagagcgtgc ggacggtcgc ggtgctcggc
      781 gcgggggtca tcgcccttga aatggcgatg atgctccaga acctggggaa gaaggtcatt
      841 ctgctgaagc actccgacca gattctgcgc cgcctcgacg gcgacgtcaa gaagaaggtc
      901 gcccagatca tgaagaaacg cggcacggac gtccgcgagt acgtgcgcct ctccgaagcc
      961 gtgcgggacg gcggaaggct gctcctgcgg ggaaccgcga aggacgagcc gttcgaggag
     1021 cggtgcgacc ggctgctgct ggcctccagc atggagccga tcctcgacgg attcgggctg
     1081 gagaacggcc ccgtccgcgt ggagaaaggg tgcatcgccg tcgacgagtc catgatgacg
     1141 tcggtcgagg gggtctacgc cgtcggcgac tgcacgggcg gagccatgct ggcccacctc
     1201 gcggagtacc aggcgctcgc cgccgtgagc cacatgacgg gcggttcgta ccgggtggac
     1261 ctcgacgccc ttccggcgtg cgtctttatc gagccggagg tcgcgacggt cggtctcacc
     1321 gaggaggagg cccgtgcgcg cggcgaagag atcgtctccg cgaaggccca cttcggcgca
     1381 aacggcatgg cgctcgccat gggcgagggg gacggcttcg tcaaggtggt cgcccgcgcc
     1441 gccgacagga cgatcctcgg agttcacatc atggggcctg aggccgcctc cctcctggga
     1501 gagaccacgc ttgcggtcgc gaagcatctg accgccgagg acgtcgcgaa cacgatccac
     1561 gcgcacccaa ccctgtgcga gtgcttcaga gacgcctgcc gccgcgtgat ggaggagtag
     1621 gcgtcctccg cagaaacgtc atcgccggag cggaatctcc tgttccttca catacacaca
     1681 acgcggccct tcgcaagagt gcgaggggcc gcgtgctgtc tcaccgagcg ccgggacttc
     1741 aatagcgagc gccgcttccc ggcggcgtca gcggcaggaa gatccgtcga gatcggcgca
     1801 gcgattgtag aggtccttga tttcgtcctc cagctgcgcg aacacctcga ccagcagcgg
     1861 gtcgaaatga ctccccgaat ccttttgcag gatggcgaaa ctttcctcgt gagagaaggg
     1921 ctccttgtac ggacgccgcg tccggagcgc gtcgtagacg tcggcgacgg ccatgatgcg
     1981 cgcgctcagt ggaatagcct cccccgagcg cccttcgggg tagccgctgc cgtcccactt
     2041 ctcatgatgg aaaagagcga tttctatccc catattcacg aacgcgttcg agacgtactt
     2101 cctccgcacc tcccgaagcg tctccgcgcc gatctcggag tgcgtcttca tgatctccca
     2161 ctcgtcgtcc gtcagccgcc ccggtttcag caggatgccg tccggaacgc cgaccttgcc
     2221 gacgtcgtgc agcggagcag cgttgtagat ggtctccgcg tactccgggg agagaagacc
     2281 gtcgtactcc ggatgctccg cgagcttcgc ggcaagcata ctgcacaggg tccgcgtgcg
     2341 ctcgatgtgc attccggtgc cgacgtcccg gtactccgtc agcttcacca tcgccagtat
     2401 cgtggagagg tgcgactctt cgatgtccct gaccttctcc tgcaccagtt cctccagctc
     2461 atgattgtag cgttcgagct cgcgctgcat ccggacgatc ttcaggtgcg tatcgacgcg
     2521 cgcgttcagc tcctcgatct ggaagggttt ggtcacataa tccaccccgc ccgccgcaaa
     2581 cgcgcgcacc ttgtcctggg aggcttcgag cgcgctgata aagagcacgg gaattttcgc
     2641 cagcgagggc tccgacttga agtggctgca cacctcgaaa ccgtccatct cgggcatgtt
     2701 gatgtccagc aggagcatat ccggaggact cttcagggcc gccttcaacg caagcttccc
     2761 ctgaggaaac gccatgatgc tgtaccctct gcgttgcagc aggttttgca gcagttcgag
     2821 gttcacgacg gtatcgtcga cgatcattat cttccctttg ctttcagcct gttcactcgc
     2881 catcggagag tcctccttca tcgttcacca gctccagcag tctctcgtag tcgtaccgct
     2941 cggcgaggag aagcagctcc ttccccgcct tttcgtccac tgcggatatg ctccggatca
     3001 actccctgat gcggtacata tcgccgtctt ccaccgcgcc gtgcaattct cgcagaagat
     3061 tccccggaat cagggaaaga tcgattgctt tcggggagcc cgggccgccg gcttccgacc
     3121 cggcctcgcc cccagccgta tctctgccgg atacatcttc gtagatatat tccagtccga
     3181 gaaccgtctg cagtacggcg aagagctcgt ccgccacgaa gggcttgcgc agatactcgt
     3241 cggcgccgca cgctgcgacg gcgtcgcggg caaaggcgtc ggcggtaagc gcgattacag
     3301 ggatattcct gctgtcgctc tctttgatca tcctcgtggc ttcgtacccg tcgaggagcg
     3361 gcatattcat atccatcagc acagcgtccg gcgtgcggga tgcgcacaat tcgacggcct
     3421 ggagaccgtt cgccgcctcg gcgcaatcga atcccgcttc ggtgagaagt tcggtcaaaa
     3481 gtccgcggct tgtatcgtcg tcgtccgcga tcagaataag gggacgcccg ctttccggtt
     3541 tcagcccaag gacgcgcccg ccgagctgcg acggccgcag ggaaacgtcg tacaccctct
     3601 ccaccggtat ccggaagcgg aaacacgacc ccttgcccag ttcgctctcg caggtcaggg
     3661 ttccgcccat catctccacg aacttccggc tgatggtgag gccgagtccg gtgccgcccg
     3721 ccttctttcc ggagtcgctc tgctggaacg gcgcgaaaat gagttcgatc tgctccggag
     3781 agatgccgac gccgctgtcc tcaacctcca ccgagagcag gagttcgcgc tcatccgagg
     3841 aagcatcccg gaaatcggtt tccgtgcgga aacgaacggt cacgcttccc tgttgcgtga
     3901 acttgagacc gtttcccaca agattgacga gcacctgccg cagttttccc tcgtcgccca
     3961 gaagcgtcgc cgcgcgcaga tcgtcgtcgt gttcgatcgt cagggagagc gacttctctt
     4021 ccgccagcgt atggaagagc gagacgatat cccgcagcat ggcgaacggg ctgaacggcg
     4081 ccctgttcaa ggtcatcctg ccggattcta tcttcgccat gtcgagaatg tcgttgatca
     4141 gcgcgagcag gtgctctccg ctgcgattga tgatccgcac gtacttcatg tgcttcggag
     4201 caagctccgg gtcgcgctcc aggagctgcg agaagcccag aatcgcgttc atcggcgtcc
     4261 ggatttcatg gctcatgctc gccaggaagg cgctcttcgc caggctcgcg gcgttcgccg
     4321 cctcaagggc tgcgcgcaga tcctcctcca tccgtttgcg ttcggtgatg tcgcggctga
     4381 tcccgagaag accgatcagc tcgccgtcct tcccccggaa ggacgttttg agcgtgtcga
     4441 gaagcgcgcg cctcccgtcc gggtaggtca cccactcctc gttatgcagc ggccgctcgg
     4501 cggctagcgc ttctttatcc ttctcccggt agaaatcggc gatacccctg tcgtaaaaat
     4561 cgtaatccgt cttcccgacc gtctccgact cgcttctacc cgtcatctcc tcgtgaggcc
     4621 ggttgcaccc gaggaagacc ccgtcggtat ttttgtaaaa aatgatatcg ggaatagagt
     4681 tcaacatgga ggcgatcagg cccgcctgtt ttctgacctc gtcctccctc tcccgccgct
     4741 c
//
LOCUS       DAOPKU010000198         4737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_19620_length_4737_cov_4.324220, whole genome shotgun sequence.
ACCESSION   DAOPKU010000198 DAOPKU010000000
VERSION     DAOPKU010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19620_length_4737_cov_4.324220"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1676)
                     /locus_tag="PK849_07295"
     CDS             complement(84..1676)
                     /locus_tag="PK849_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HPE65968.1"
                     /translation="MGPQLKSGHVVGLIDLGTNSVRLFVVRINENKSYTILNQHKIMV
                     RLGEGEFVKQRLKNEAMERTILVLGRFAEMARNLGAEEILAVATSATRDAENKDVFLE
                     RVRKEAGIDLKVISGLEEARLIYLGIASGMHLGDKTALFIDIGGGSTETIVGNQAEYF
                     HLDSLKLGAVRLASLFFLPGETDPVSPDRYALLQQYVRNASLRSVQRLREFPVDLVVG
                     SSGTLETLAEMAFRRGNKKQKGASAEKESSERTMTRRQLEELTKLLCSLSLEERKRLP
                     GMVPERADIIVAGASIIHMLMEDLELDKIRISTRSLRNGLLVDYLMRGKWGYLDAAFS
                     VREQSVLQLARSCGFHEEHSRHLGDLCMQLFDSGREAGLHSFGESAKELLFYAALLHD
                     IGLFLSFSNHHAHSHYIIRNVELLGFHDNEIAVIAALAFYHRKRSPKKKDPEFEALDQ
                     DSQRIVFTLSMFLRMAESLDRSHHNFVHEVRFVREKNVLLLKIQADKGYQLELWALEE
                     HQKYFVKTFGERFGIQVTASGF"
     gene            complement(1661..2947)
                     /locus_tag="PK849_07300"
     CDS             complement(1661..2947)
                     /locus_tag="PK849_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HPE65969.1"
                     /translation="MKIILLGDVHGNLPALEAVLEDGRRRGGDIVLNTGDYVGQGPFP
                     DETVSLLSSVESVAVAGNFDRNVLRAKQGKTGEKAELHLWNRGRLSRESRKYLKNLPR
                     TKKFLLAGKQFLLTHGSPEDIEENIGPDTPDERLCEIARLASSDIVLTGHTHAPLSKK
                     IGDTWFINPGTAGRPLGDDLRACYALLHIQPGYFRVNHYLVDYDMAKTADAALFRGMP
                     GDFASSFLPGRAVAGDIPMEDEAERILETTIPVHADHSRHVASIALALFDRLEELHGL
                     DREARFQLQIASTLHDIGWLKGQKEHHKVAMEFILGLDGVIPDERERILIASVARYHR
                     KADPDDSHPFFERLSPGDRETVRKLAAILRIADGLDWTHSSVVKGVECLVGDGAVTVL
                     CSTSGDAGAERARALEKGALFEKVFGKKLDIEWVRS"
     gene            complement(2944..3921)
                     /locus_tag="PK849_07305"
     CDS             complement(2944..3921)
                     /locus_tag="PK849_07305"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HPE65970.1"
                     /translation="MKERAPTLRAFAVAALVPLLEKLSSQLEDLRTEEDIECLHKARV
                     STRRLRASLSLFAPCFPSGEAKRWKKAVTRITALLGEARDLDVQMESVEEELRATGDR
                     ERPGLARLLLRLSRRREKLQRRICAALDGRDIREPLDEMLDGLREAVRKDILERDDSP
                     SFALCADEIRAHVADVLSFDGFVRNPASIKELHDLRKAVKRLRYALEIFEPLSAGLLR
                     PFVERLKALQDLLGEIHDCDVWLDFLPRFLEEEAARTLKYYGHVRVFPPLKTGILAFM
                     EKKRTLRGEDFGRFLDEWGRLGKEAFWDNLNTAAETLRAYRGEEGADHS"
     gene            complement(3918..>4737)
                     /locus_tag="PK849_07310"
     CDS             complement(3918..>4737)
                     /locus_tag="PK849_07310"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659147.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HPE65971.1"
                     /translation="RFRAGLVGYKIHAKMCLVIRRESDGVKRYAHIGTGNYNPSTAKV
                     YSDLGLFTADKAICADVTDLFNAITGYSRKEDYRQLLVSPHTTRSGIVGRVDREIARH
                     KESGDGYIAFKMNQLVDKECIEALYRASQAGVRVRLQIRGICCLRPGVPGVSETIDVT
                     SIVGRFLEHPRMFYFRNGGDDELFIGSADLMPRNLDRRVEVLVPVREKDLRETLLRDM
                     LDVHLTDSRKSWRLLSDGTYRRITPEEGKPVIDSQKIMMQRSTGWNPPERSDER"
BASE COUNT          811 a         1436 c         1536 g          954 t
ORIGIN      
        1 gccagaagaa acggtttcac ttccggccta aatttcatta aaaagccccc ttgtgtgtat
       61 atggattcac cttacattat accctagaaa ccggatgcgg tcacctgaat accgaaacgt
      121 tctccaaatg tcttgacaaa atacttttgg tgttcttcaa gagcccagag ttcaagctga
      181 taccccttgt cggcctgtat tttgagcaga agcacgtttt tttcgcggac aaagcgaacc
      241 tcgtgcacga aattgtggtg gcttcggtcg aggctctcgg ccatccggag gaacatgctg
      301 agggtgaaga cgatgcgctg cgaatcctgg tcgagggctt cgaactcggg gtcttttttc
      361 ttcggcgagc gctttctgtg gtagaaggcg agcgcagcga tgacggcgat ctcgttgtcg
      421 tggaagccca gaagctcgac gttgcggatg atgtagtgac tgtgggcgtg gtggttgctg
      481 aaggagagga agagcccgat gtcgtggagc agcgccgcgt agaagagcag ctccttcgca
      541 ctctcgccga aggagtgcag cccggcctct ctcccgctgt cgaagagctg catgcagagg
      601 tctccgaggt ggcgcgagtg ctcctcgtgg aagccgcacg agcgcgcgag ctgaaggacg
      661 ctctgttcgc gcaccgagaa tgcggcgtcg agataccccc actttccgcg catcaggtag
      721 tccaccagca gaccgttccg gaggcttcgc gtgctgatcc gtatcttgtc cagctcaaga
      781 tcctccatca gcatgtggat gatggaggcg ccggcgacga tgatgtccgc acgttcagga
      841 accatgccgg gaaggcgttt ccgctcttcg agcgagaggg agcagaggag ctttgtcagt
      901 tcctcgagct ggcgccgggt catggtccgt tcggagcttt ccttctcggc ggaggcgcct
      961 ttttgtttct tgttcccgcg ccggaacgcc atttccgcca gcgtttccag cgtgccggag
     1021 cttcccacga cgagatcgac cggaaattca cggagcctct ggacgcttcg aagcgaggcg
     1081 ttccggacgt actgctgcag cagcgcgtag cggtccggag agactggatc ggtttccccg
     1141 gggaggaaga agagcgacgc gaggcggact gcgccgagtt tgagggagtc aaggtggaag
     1201 tactccgcct ggttgccgac gatcgtctcc gtgctgccgc ctccgatgtc gatgaagagc
     1261 gccgttttgt cgccgaggtg catgccgctc gcgatgccga ggtagatcag gcgggcctcc
     1321 tccagtccgg agatgacctt caggtcgatt cccgcttcct tgcgcacgcg ttccaggaaa
     1381 acatccttgt tctccgcgtc ccgcgtggcg gaggtagcca ccgcaagaat ctcctccgcg
     1441 ccgaggtttc gcgccatctc ggcgaatctg ccgagcacaa ggatcgtccg ctccatggcc
     1501 tcgttcttga ggcgctgctt gacgaactcc ccctcgccga ggcggaccat gatcttgtgc
     1561 tggttcagta tcgtgtagga tttgttctcg ttgatgcgca cgacgaaaag gcgcacggag
     1621 ttcgtcccga ggtcgatgag gccgaccacg tgccccgact tcagctgcgg acccattcga
     1681 tatccagctt ctttccgaag accttctcga agagcgcgcc cttctccagg gcccgcgctc
     1741 tctccgcccc ggcgtctccc gacgtcgagc agaggacggt cacggccccg tcgccgacga
     1801 gacactccac ccctttgacg accgacgagt gggtccagtc gagcccgtcg gcgattctga
     1861 ggatcgctgc gagcttccgt acggtctctc tgtccccggg tgagagacgc tcgaagaagg
     1921 ggtgactgtc gtcggggtcg gcttttctgt ggtagcgggc gacggaggcg atcaggatgc
     1981 gctcccgctc gtcggggatg acgccgtcga gtccgaggat gaactccatg gcgaccttgt
     2041 ggtgctcctt ctgccccttc agccagccga tgtcgtggag cgtcgaggcg atctggagct
     2101 ggaagcgcgc ctcccggtcg agtccgtgaa gctcttcgag gcgatcgaag agggcgaggg
     2161 cgatggacgc cacgtggcgg ctgtggtcgg cgtgcaccgg aatggtcgtc tccaggatgc
     2221 gctcggcctc gtcctccatc gggatatccc ccgcgacggc ccgtcccggc agaaaggagg
     2281 aggcgaagtc ccccggcatc ccccggaaga gcgccgcgtc ggccgtcttc gccatatcgt
     2341 agtcgacgag gtagtggttc accctgaagt agccgggttg gatgtgcagc agcgcgtagc
     2401 aggcacggag gtcgtctcca agggggcgtc cggccgttcc cgggttgatg aaccacgtgt
     2461 cgcctatctt tttcgagaga ggggcgtggg tatgcccggt aagcacgatg tcggaggagg
     2521 ccaggcgggc gatctcacaa agacgctcat cgggagtgtc cgggccgatg ttctcctcga
     2581 tgtcctcggg ggagccgtgc gtgagcagaa attgcttgcc cgcgagcagg aatttcttcg
     2641 tccgggggag gtttttgagg tacttgcggc tttcccttga aaggcgtccc ctgttccaga
     2701 ggtgcagttc ggccttctcc cccgtctttc cctgcttcgc ccgaaggacg ttccggtcga
     2761 agttccccgc gacggccacc gattccaccg aggagagcag cgagacggtc tcgtccggaa
     2821 acggcccctg tccgacgtaa tcgccggtgt tgagcacgat gtcgcctcct cgtctgcgtc
     2881 cgtcctccag gacggcctcc agcgcgggga gattgccgtg gacgtctcca agcagtatga
     2941 tcttcacgaa tgatccgctc cttcctctcc gcggtacgct cgaagcgtct ccgcggcggt
     3001 gttcagatta tcccagaacg cttccttccc gaggcgcccc cactcgtcga gaaaacggcc
     3061 gaaatcctct ccccgcagcg tccgtttctt ctccatgaag gcgaggatgc ccgtcttcag
     3121 cgggggaaag acccgcacat gtccgtagta cttcagcgtc ctcgccgctt cttcctccag
     3181 aaaacggggg agaaaatcaa gccagacgtc gcagtcgtga atctctccaa ggaggtcctg
     3241 gagggccttc agcctctcga cgaaggggcg aagaagcccc gcggagagag gctcgaagat
     3301 ctccagcgcg taccggagcc gcttcacggc cttcctgagg tcgtgcagct ccttgatcga
     3361 cgcggggttc cggacaaacc cgtcgaagct gaggacgtcg gcgacgtgcg cgcggatttc
     3421 gtccgcgcag agggcgaacg agggggagtc gtcccgttca aggatgtcct tgcggaccgc
     3481 ttcccgaagg ccgtcgagca tttcgtcgag aggttcgcgt atatcgcggc cgtcgagcgc
     3541 ggcgcagatg cgccgctgca gcttctccct gcgccgcgag aggcgcagca gcagacgggc
     3601 gagcccggga cgctcgcggt cgccggttgc gcgaagttcc tcttcgacgg actccatctg
     3661 gacgtcaaga tcccgggctt ctccgaggag ggctgtgatc ctggtcacgg cctttttcca
     3721 gcgcttggcc tctccggagg ggaagcaggg agcgaagagg gagagagagg cgcggagccg
     3781 ccgggtggag accctcgcct tgtgaaggca ttcgatatcc tcctctgtcc ggaggtcctc
     3841 cagctgcgag gagagtttct ccagcagcgg gacgagcgcg gcaacggcga aggcgcggag
     3901 ggttggggcg cgctccttca tcgttcgtcg ctcctttccg gcggattcca ccccgtactt
     3961 cgctgcatca tgatcttctg cgagtcgatc acgggctttc cctcctccgg cgtgatcctg
     4021 cgataggtcc cgtcggacaa gagtctccac gacttcctgc tgtctgtgag atgcacgtcg
     4081 agcatatcgc ggagcaaagt ttcacgaagg tccttctccc tgacggggac gagcacctcg
     4141 acgcggcggt ccaggttgcg cggcatgagg tcggcgcttc cgatgaacag ttcgtcgtcg
     4201 ccgccgttgc ggaagtagaa catccggggg tgctccagga aacgtccgac gatggaggtg
     4261 acgtcgatgg tctcgctcac gcccggaacg ccggggcgga ggcagcagat gccccgtatc
     4321 tgaaggcgca cgcgcacccc cgcctgcgag gcgcggtaga gcgcctcgat gcactccttg
     4381 tcgacgagct ggttcatctt gaacgcaata tacccgtctc ccgattcttt atgccgcgcg
     4441 atctcgcggt cgacccgccc gacgatgccg ctccgggttg tgtgcgggga gacgagaagc
     4501 tggcggtagt cctccttgcg cgagtagccg gtgatggcgt tgaaaaggtc ggtgacgtcc
     4561 gcgcagatgg ccttgtccgc ggtgaagaga cccaggtcgg agtagacctt cgccgtggag
     4621 gggttgtagt ttccggtgcc gatgtgcgcg tagcgcttga cgccatcgct ttcgcgccgg
     4681 atcacgaggc acatcttcgc gtggatcttg tagccgacga ggccggcgcg aaagcga
//
LOCUS       DAOPKU010000199         4733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_19643_length_4733_cov_4.466866, whole genome shotgun sequence.
ACCESSION   DAOPKU010000199 DAOPKU010000000
VERSION     DAOPKU010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19643_length_4733_cov_4.466866"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PK849_07315"
     CDS             complement(<1..468)
                     /locus_tag="PK849_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE65972.1"
                     /translation="MAKERILIVGGDAAGMSAAGQIRKLLPDSEIVVYERSGYSSYSA
                     CGIPYFIAGLVEPETRLVVRTPREFLEKQNIVVHVRHEVLSLDPGAGKVKVRDLETGR
                     EFADGFDKLLLATGASPVVPPLNGAKADGIFSLGVLETGIAARKFIDQHRPRHA"
     gene            complement(544..1803)
                     /locus_tag="PK849_07320"
     CDS             complement(544..1803)
                     /locus_tag="PK849_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE65973.1"
                     /translation="MPDWEKSTLFRAFRHRNFRLFYMGQIVSLSGLWMQRVAMNWLVY
                     RITGSPLLLGTVDFMAQIPVLLFGSFTGAFLEGRNLRKVIMICQTISMLHALTLAILT
                     LSGVVEYWHILILAAVIGTSDSFEVPARQGFTIRLVEDEQDLGNGIALTSTLFNVTRL
                     IGPSVAGMVIALVGEGICFLMNGMAYSATLTALFLMRFKRPLVSESTKKGVLEGMKEG
                     IRYVAGFLPLRNYLSAIALVSFFAFPYMVLLPVFAREILGGGPRTLGFLMGATGLGAL
                     AGSVRLALRKSPVGLGKIMAVSCILLGITMALFSQSGIFPLSLLLMACLGFFMVSTLV
                     SCNTLVQTLVDEDKRNRVMSLFIVCAMGITPLGSLNAGWLASNIGAPTTLLAGGAVSF
                     LIGLWLLRSWPVMWALSEPVYRKKNLL"
     gene            complement(1810..2694)
                     /locus_tag="PK849_07325"
     CDS             complement(1810..2694)
                     /locus_tag="PK849_07325"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPE65974.1"
                     /translation="MSLTFSPLSIDMRSDYTPLFDKCSEKTSDYTFVNIWAWSDERKY
                     ELAFARDLFWPRVTSSAPVYWAPVGNWEATDWESVLPELFPDGMTFDRVPEKLAQLWS
                     ERLGDRVTLEEQRSEWEYVYSVEELVALRGNRFHKKKNLLKQFQREYVSEYKALGRDD
                     VEEILEMQQLWCEWKSCDDIPGLRAENHAISRVLKKWDLLPGLLCGALYVKEYMVAYA
                     VGEPMTDDMVVVHFEKGLAEYKGVYQAINQSFLANSCSGFAWANREQDMGEEGVRKAK
                     ESYNPSHFLKKYRAVWKG"
     gene            complement(2830..4719)
                     /gene="dnaK"
                     /locus_tag="PK849_07330"
     CDS             complement(2830..4719)
                     /gene="dnaK"
                     /locus_tag="PK849_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPE65975.1"
                     /translation="MKKAVGIDLGTTNSVIAVIEGGTPEVIANAEGGRTTPSVVAFTK
                     DGERLVGSLAKRQATLNPEGTLFSVKRFIGRKYNEVASELKIVPYKVNEGANGAVRFS
                     VGGKEYAPEEISASVLRKLVSDASSYLGEKITDVVITVPAYFNDAQRQATKDAGTIAG
                     LNVLRVVNEPTAAALAYGLDKKKEQKIMVFDLGGGTFDVSILDIGDGVVEVLSTSGDT
                     HLGGDDFDKVVVDWIADEFRKENGIDLRKDKQALQRLYEAAEKAKCELSSRTSTEISL
                     PFITADANGPRHLNMAITRAKFDQLTAPLVERCAGPVKDALSAAKLSEKDIDEVVLVG
                     GSTRIPAVQDVVRKLTGGKQPNMSVNPDEVVAVGAAVQAGIISGEMKDVLLLDVIPLS
                     LGVETMGGVMTKLIEKNTTIPCRKAEIFSTAADNQPAVDIVVLQGEREMAKDNRILGN
                     FKLEGIRQAPRGLPQIEVAFDVDANGILNVTAKDKDTAKEQRITINGSTNLDKSEVER
                     MIRDAQENAAEDRKKKELAEARNELETVVYQLEKALEQAAANVPENEKARARNLVEEG
                     KSALSKSDATLDALRVASSDIKQMLQILASSNAGGPEPQPASSARSGDDVVDAEFVDN
                     SENRG"
BASE COUNT          970 a         1463 c         1300 g         1000 t
ORIGIN      
        1 tgcgtggcgc gggcgatgct ggtcgataaa cttgcgtgcg gcgatgcccg tctcaaggac
       61 gccgaggctg aagataccgt ccgccttggc gccgttcagc ggcggcacga cgggactcgc
      121 gccggtcgcc aggagcagtt tgtcgaaacc gtcggcaaac tctctgcccg tctcaagatc
      181 gcgaaccttc accttgcctg cgccgggatc aagcgaaagc acttcgtgcc tgacatgaac
      241 gacgatattt tgcttttcca ggaactcccg gggcgtgcgg accaccaggc gcgtttccgg
      301 ttcgacgagc ccggcgatga agtaagggat tccgcacgct gaatatgagg aatagccgct
      361 tcgctcatag acgacaattt cagaatcggg cagcagtttc ctgatctgac ccgcggcgga
      421 catgccggcg gcgtcgccgc cgacgataag aatacgctct ttcgccattc cgatcacctc
      481 cgcaatacct taaatgggta tttttttctt cttcagagta taccccaaat ccgggggaac
      541 gcgctacagc agattcttct ttctatacac cggctccgag agcgcccaca taacgggcca
      601 cgaacgtaac agccacaagc cgatgagaaa actgacggcg ccacccgcaa gaagggtcgt
      661 cggcgctcct atgttcgagg cgagccatcc ggcgttgagg ctccccagcg gtgttatgcc
      721 catcgcgcac acgatgaaga ggctcatgac gcggtttcgt ttgtcctcgt ccacaagagt
      781 ctggacgagc gtattgcagg aaacgagcgt ggagaccatg aaaaaaccaa ggcacgccat
      841 caggagcagc gaaagaggga agatcccgga ttgggaaaag agcgccatcg taattcctaa
      901 aagaatgcag gagacagcca tgatcttgcc cagtccgact ggcgactttc gcagggcgag
      961 acggaccgac ccggcgagcg cgccaagccc ggttgcgccc atcaggaaac caagcgtccg
     1021 gggtccgccg ccgagaatct cgcgggcaaa gacggggagc agcaccatgt acgggaaggc
     1081 gaagaagctc acaagagcga tcgcggagag gtagttcctc aggggcagaa agcccgcgac
     1141 gtaacgaatt ccctccttca tcccttcgag cacccccttc ttcgtacttt ccgaaacgag
     1201 cggacgcttg aagcgcatca ggaagagcgc cgtgagcgtc gccgaatagg ccataccgtt
     1261 catcaggaag cagatgccct cccctaccaa tgcgatgacc ataccggcga ccgacggacc
     1321 gatgagacga gtgacgttga aaagagtgga cgtgagggcg atgccgttgc cgagatcctg
     1381 ttcgtcctcc accaggcgta tcgtaaaccc ctgccgggcc ggaacctcga aagaatccga
     1441 cgttccgatg acagcggcga gtatcaggat atgccagtac tcgacaacgc cgcttaacgt
     1501 cagaatggca agcgtaaggg cgtggagcat gctgatggtc tgacagatca tgatgacctt
     1561 ccgcaggttc cgcccctcca gaaaggcccc cgtaaaagag ccgaagagca gcacgggaat
     1621 ctgcgccatg aaatcgaccg tccccaaaag cagcggcgaa cccgtgattc tgtagacaag
     1681 ccagttcatg gcaacccgtt gcatccagag tccggagagc gagacgatct gccccatgta
     1741 gaacaggcgg aaattccggt gccggaaggc ccggaagagc gtgctcttct cccagtcggg
     1801 catatcgctc taccccttcc agacggcgcg gtacttctta aggaagtgcg acgggttgta
     1861 cgattccttg gccttccgaa ccccctcctc gcccatgtcc tgctcgcggt tggcccacgc
     1921 gaagccggaa caggagttcg ccaggaagga ctggttgatc gcctggtaga cgcccttgta
     1981 ctccgcaagc cccttctcga aatgcaccac gaccatgtcg tccgtcatcg gctcgcccac
     2041 ggcgtaggct accatgtact ctttaacgta gagcgcgccg cagagcaacc ccggaaggag
     2101 atcccacttc ttcaagacac gcgaaatcgc gtggttttcc gctctcaagc cagggatgtc
     2161 gtcgcagctc ttccactcgc accaaagctg ctgcatctcg aggatttcct caacatcgtc
     2221 gcgtccgagc gccttatatt cagaaacgta ctccctctga aactgcttca ggaggttctt
     2281 tttcttgtga aaacggtttc ctcgaagcgc cacaagctcc tccacggagt agacgtactc
     2341 ccattccgaa cgctgttcct ccagcgtcac gcggtcgccc agacgttcgc tccaaagctg
     2401 cgcgagtttc tccggtaccc ggtcgaacgt catgccgtca gggaagagct ccggcagcac
     2461 actctcccaa tcggtcgcct cccagttacc caccggcgcc cagtacaccg gagcggaact
     2521 cgtcacgcga ggccagaaaa gatccctggc gaaagccaac tcatacttgc gttcgtccga
     2581 ccatgcccag atattcacga acgtgtagtc ggatgttttt tcggaacatt tgtcgaaaag
     2641 aggggtgtag tcgctcctca tatctatcga aagcggcgaa aaagtcaatg acatcctgtc
     2701 acctccagta atgaaagtag tatacccgta aagaagcctg cgggcaattt ccccgcaggc
     2761 ttctctctat tgaaaaccgg caaatcgccg gaagtgtgtt ttcaggccgc cggcccttcg
     2821 ttaaaagcct cagccgcggt tttcggagtt atccacaaat tcggcgtcca caacgtcgtc
     2881 gccgcttcta gccgaggatg cgggctgcgg ttcagggcct ccggcgttcg acgacgccag
     2941 aatttgcagc atctgcttga tatcggagga ggcgacgcga agggcgtcca gcgttgcgtc
     3001 gctcttggac aatgcagatt ttccctcctc cacgaggtta cgggcccggg ccttctcgtt
     3061 ctccggtacg ttcgccgccg cctgttcaag cgccttctcc aactggtaga caaccgtttc
     3121 cagttcattc cgggcctcgg cgagctcttt cttcttcctg tcctcggcgg cgttctcctg
     3181 ggcatcgcgg atcatgcgct ccacttcgga tttgtcgaga tttgtcgacc cgttgatggt
     3241 gatacgctgc tcctttgcgg tatccttatc cttcgccgtg acgttcagga tgccgttggc
     3301 gtcgacatcg aaagccactt cgatctgcgg caaccctctg ggggcctgcc gtattccctc
     3361 aagcttgaag ttgccgagga tgcggttatc cttcgccatc tcacgttcgc cctggagcac
     3421 cacgatgtct accgcaggct ggttgtccgc cgctgtcgag aatatttccg ctttcctgca
     3481 cggaatcgtg gtatttttct cgatgagctt cgtcatcacg ccgcccatgg tctccacgcc
     3541 gagagagagc ggaataacgt cgaggaggag cacatccttc atctcgccgc tgataatgcc
     3601 ggcctgtacc gccgcgccta cggccaccac ttcgtccggg ttcaccgaca tattgggctg
     3661 tttaccaccc gtgagtttgc ggacaacatc ctgaaccgcc ggaatgcgcg tggaacctcc
     3721 tacgaggacc acttcgtcga tatccttctc ggagagcttg gccgcggaga gcgcgtcttt
     3781 tacggggccg gcgcagcgct ctacgagggg agcagtaagt tggtcgaact tggcccgcgt
     3841 aatcgccatg ttcagatgtc tggggccgtt tgcgtcggcg gtgatgaagg ggagactgat
     3901 ctccgtgctc gtcctcgacg agagctcgca tttcgccttc tccgccgctt cgtagagccg
     3961 ctggagcgcc tgcttgtcct tgcggaggtc gatgccgttc tccttgcgga actcgtcggc
     4021 aatccagtcg acgacgacct tgtcgaagtc atcgcctccg aggtgggtgt cgccggaagt
     4081 agaaagcact tccactaccc cgtcgccgat gtcgagaata gagacgtcga acgtgcctcc
     4141 tccgaggtcg aagaccatga ttttctgttc ttttttcttg tcgagtccgt aggcaagagc
     4201 cgccgccgtc ggttcgttca ccacgcggag cacgttcagc ccggcgattg tccccgcatc
     4261 cttggtggct tggcgttgcg cgtcgttgaa gtaggccggc acggtgatga cgacgtccgt
     4321 aatcttctcg cccaaatagg aggacgcgtc gcttaccagc ttgcggagca cggaggcgct
     4381 aatctcctcg ggagcatact ccttccctcc gaccgaaaac ctgacggctc cgttggcccc
     4441 ttcgttcacc ttgtacggga caatcttcag ctcggaagcc acctcgttgt atttccgtcc
     4501 tataaatcgc tttacagaaa agagcgttcc ttccgggttc aatgtcgcct gccgttttgc
     4561 aagcgatccc accagacgct cgccgtcttt tgtaaaagcg acaaccgaag gagttgttcg
     4621 ccctccctcc gcgttcgcga tgacttccgg agtaccacct tctataactg cgatgacgga
     4681 gttcgtcgtt ccaagatcta tgccgaccgc ctttttcatt tcgtatcccc cct
//
LOCUS       DAOPKU010000200         4657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20119_length_4657_cov_5.071056, whole genome shotgun sequence.
ACCESSION   DAOPKU010000200 DAOPKU010000000
VERSION     DAOPKU010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20119_length_4657_cov_5.071056"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..88
                     /locus_tag="PK849_07335"
     tRNA            14..88
                     /locus_tag="PK849_07335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Val,seq:gac)
     gene            359..1354
                     /locus_tag="PK849_07340"
     CDS             359..1354
                     /locus_tag="PK849_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE65976.1"
                     /translation="MKALRVLFALIVLLVFALSSGASQLSIAMITDVGGVNDQSFNQS
                     AWEGLQRAEKDLGVKAAYIESRQDADYGPNLETLLDAGTDLIWGIGFKMADAVLEAST
                     NNVDQKYAIIDFAWEKTPENLIGVVFKAEEASFLVGYIAAKMTKTGKVGFIGGMSFPV
                     IWGFEYGFRAGVAQAGKNVEILSQYAESFTDAAKGKSIANSMYQQGADIIFHASGGVG
                     DGLIEAAKEQGKWAIGVDRDQNYLAPENVLTSAMKRVDNAIFNIAKELIEGKWQGGHT
                     VSYGLAEGGVDIAPTTSKHVPAELLAEVEAVKARIIAGEVVVPFSKETFASFEVK"
     gene            1423..2967
                     /locus_tag="PK849_07345"
     CDS             1423..2967
                     /locus_tag="PK849_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65977.1"
                     /translation="MPERKKNAAELAVEMKQITKRFGNFVANDSIDFDVRRGEVHALL
                     GENGAGKSTLMNQLYGLYLPSSGEIFINGKKVRITGPKIAIARGIGMVHQHFMLVQPF
                     TVAENIVLGCESVRAFGILDTTRAFREVEDLSRRYGLAVDPGARIQDISVAMQQRVEI
                     LKCLYRGAEILILDEPTAVLTPQEIDFLAEILQNLTAEGKTVIIITHKLREIKKMAHR
                     CTVIRRGVKIGTVDVASRSESELAEMMVGRAVSFDVEKTPARVQEPVLLLTDIYALNN
                     RGLPALKGISLVVKRGEILALAGIDGNGQSELVEVLTGLRPVESGSIRFRNTEITDFP
                     SGKRIDMGLAAIPEDRQKRGLIGPFTIAENLILEERSLPPFSRSGILDLQAISRHAKT
                     LIDRFDIRPPREQYLATSLSGGNQQKVILAREITRDPELLIASQPTRGLDVGAIEYVR
                     KRLVQVRDAGKAVLLISLDLDETLALADRIAVIYEGRIVAVLDREETDERTLGLLMAG
                     GGAVHV"
     gene            2960..4099
                     /locus_tag="PK849_07350"
     CDS             2960..4099
                     /locus_tag="PK849_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE65978.1"
                     /translation="MSDEKRKLLAASPLFFTFFSVLTGLLVGALVLLISGYSPLKAYH
                     VIIDGIFSRPSYIVYTVIYSTPLIMTGLSVAFAFRTGLFNIGAEGQFIMGALGAALAG
                     SFLHLPPVLHAIVAALAGIAAAAVWGGIAGFLKARFGVNEVIATIMLNWTALYLNNFI
                     VTLPSIKRTATNAMAFILDSARIEILPLWKVSEAGRTWRAMHPFWHDIFRTPFNAGIL
                     VALFLAFFCWWILNRTTLGYELRAVGHNADCSEHTGINVKARMISSMAIAGGLAGAAG
                     ALHVLGWTRQAAELAAMEGLGFDGIAVALIGGTSPIGCIPAGLLFGALKYGGSKLQFA
                     LRAPIEVVNIVIGSIIFFVAMPKLLRIIRRWLEPKKRQGGDSHAA"
     gene            4089..>4657
                     /locus_tag="PK849_07355"
     CDS             4089..>4657
                     /locus_tag="PK849_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE65979.1"
                     /translation="MLLESIAFIIATTLMYSAPLIFTALGGVVSENAGVVNIGLEGMM
                     TLGAFVGAAVGYFANDAWIGFLAAGAAGGALAFFHALACVTFLADHIVSGIAINFIGP
                     GLSLFLSRYFFDGATQTLSLSLDAKIPRPLNGVFEQNGFFDMVFNQYATVYLALFLVA
                     FLWFILYRTRLGLRIRAVGEHPKRRIPWV"
BASE COUNT         1054 a         1280 c         1244 g         1079 t
ORIGIN      
        1 atatctcctc gttgggtggt tagttcagag gaagaacgct tccttgacgc ggaagaggtc
       61 agaggttcga gtcctctacc acccaccatt gactactaaa gagcttcagc tatctgctgg
      121 agctcttttt gtatttgaaa gcagtctata ttctcttaca cactgcctcc ttcactcaaa
      181 gacaatctgc tctttacagt atcctctttt catcaaaact gattatccca ttgaaaacac
      241 gcttcgagag ccgcggatgg cgcttgcgga aatttttcca gggatgtaag attgttgaaa
      301 tattgaagcc ggtttttctt ccggcacgta atactatccc aagagaggag ttggtctaat
      361 gaaagcttta cgggtgctgt tcgctttgat tgtccttctt gtttttgcgc tgtcttcggg
      421 ggcatcacaa ctatcgattg caatgatcac agacgtaggg ggcgtgaacg atcaatcctt
      481 caaccaatca gcatgggaag ggcttcaaag agctgaaaaa gatctgggtg tgaaggcagc
      541 ctatatagaa tcgcgtcaag acgccgacta cggcccaaac cttgaaaccc tgctcgacgc
      601 aggaacggac ctgatctggg gaattggttt caaaatggcc gacgcagtac tggaagcttc
      661 cacgaacaac gtcgatcaga aatacgccat tatcgacttc gcctgggaaa aaacaccgga
      721 aaaccttatc ggcgttgtct tcaaggctga agaagcatcc ttcctcgtcg gttacatagc
      781 ggcgaagatg acgaagacag gaaaggtcgg cttcattggc ggcatgtcat tccctgtgat
      841 ctggggattc gaatacgggt tccgcgcagg tgttgcgcaa gctggaaaga acgtcgaaat
      901 cctgagccag tacgcagaat ccttcactga cgctgcaaag ggcaaatcca tcgccaacag
      961 catgtaccag cagggcgccg acatcatctt ccatgcatca gggggcgtcg gcgacggtct
     1021 gatcgaagcg gctaaggaac aggggaagtg ggcgattggc gttgacagag accaaaacta
     1081 tcttgctccc gagaatgtcc tcaccagcgc catgaaacgg gttgacaacg cgatattcaa
     1141 catcgcaaag gaactgatcg agggtaaatg gcagggcggg cacacggttt cttacggact
     1201 cgcggagggc ggagtagata ttgcccccac aacatcaaaa cacgttccag cggagctgct
     1261 cgcggaagtg gaagctgtta aagcgcgaat catcgccgga gaagttgtcg tcccattcag
     1321 caaagagacc tttgcttcct tcgaggtgaa atagaaacga gcgcggggga agtacatcaa
     1381 aaaatcctcc gcgcaccttt tctattctga ggaggtgggg ttgtgccgga acgtaaaaag
     1441 aacgctgccg aactggcggt agagatgaaa caaattacaa agcgattcgg aaacttcgtc
     1501 gcaaacgact caatcgattt cgatgttcgc cgcggcgagg tccacgcgct cctcggtgaa
     1561 aacggcgcgg gaaaaagcac gctgatgaac cagctctacg gcctttatct tccttcttcg
     1621 ggagagattt tcatcaatgg taaaaaagtc cggataaccg gccccaagat agccatcgcc
     1681 aggggaatag gaatggtgca tcagcatttc atgcttgtgc agccgtttac cgtagccgag
     1741 aacatcgttc tcggctgtga atcggttcga gcattcggca tccttgatac cacacgtgcg
     1801 ttccgagagg tggaagacct ttcgcgccgt tacgggctcg ctgtcgatcc tggcgcacga
     1861 attcaggata tttctgtggc tatgcagcag cgcgtggaaa ttttgaagtg cctctaccgt
     1921 ggggcagaaa tcctcatact cgacgaaccg acggcagttc ttactcccca ggagattgat
     1981 tttcttgccg agatactcca aaatctgact gctgagggga aaacagtcat cattatcacc
     2041 cataaactac gagagataaa aaaaatggcg catcgctgca ccgttatcag gaggggcgta
     2101 aagataggga ctgtcgacgt tgcgtcccgc tccgagagcg agctggccga aatgatggtc
     2161 ggacgcgccg tttctttcga tgtcgagaaa acgcctgcaa gagtgcaaga gcctgtgctc
     2221 ttgcttactg atatttatgc cctcaataac aggggacttc ctgcgctcaa aggtatatcg
     2281 ctcgtagtaa aacgaggtga gattctggct cttgcaggaa tcgacggaaa cgggcagagc
     2341 gagcttgtag aggtcttgac cggcctccgc cccgtcgaat ccggctccat ccgtttccgc
     2401 aacactgaga tcacggattt cccatccgga aagagaatcg acatgggctt ggcggcaatc
     2461 ccggaggatc gccaaaaacg cggcctcatc ggccccttca cgatcgccga gaaccttatc
     2521 cttgaggagc gctcactccc gcctttttcg cgcagcggta ttcttgatct gcaagcgatc
     2581 agccgtcatg ccaaaacgct cattgatcgt ttcgatattc gccctccccg cgaacagtat
     2641 ctcgctacgt cgctctccgg aggaaaccag cagaaggtca tcctcgcaag ggaaatcaca
     2701 cgcgatccgg agctgcttat cgcgtcgcag ccgacaaggg gacttgacgt gggcgccatt
     2761 gaatacgtac gcaaaagact tgtccaggtt cgcgacgcag ggaaagctgt tctccttatc
     2821 tctctcgatc tcgacgaaac gcttgctctc gccgaccgta tcgcagtcat ctacgaaggc
     2881 cgcatcgttg ccgttcttga ccgggaagag acagacgagc gcacgctcgg gcttctcatg
     2941 gcaggaggtg gagcagtgca tgtctgacga aaaaaggaag ctacttgcag cttcgcctct
     3001 cttcttcact tttttttcag tgctcacagg gcttcttgtc ggcgccctcg tgcttctgat
     3061 cagcggatac agccctctca aggcgtatca tgtgatcatc gatgggattt tctccagacc
     3121 gagctatatc gtctacacag tcatttactc cacgccgctc atcatgacgg ggctctcagt
     3181 cgctttcgct ttccggacag gtcttttcaa tatcggggcg gagggacagt tcatcatggg
     3241 cgccttgggc gcggcactcg caggctcttt tctacacctg cctcctgtgc tccacgccat
     3301 cgtcgccgca ctggcgggca tcgcagcggc tgccgtctgg ggaggcatcg cgggctttct
     3361 taaagcgcgc ttcggcgtca acgaagtcat cgccacaatt atgctcaact ggacagccct
     3421 ctacctgaac aactttattg ttacgctgcc gtcaatcaaa cgaactgcaa caaatgctat
     3481 ggcattcatt ctggactcgg cgcgaatcga aatactgccg ctgtggaaag taagcgaagc
     3541 gggaagaacc tggcgcgcta tgcatccatt ctggcacgac attttcagaa cacccttcaa
     3601 tgccggaata ctggtcgcgc tcttccttgc ttttttctgc tggtggatcc tgaacaggac
     3661 gacgctcggt tacgagcttc gggccgtcgg tcacaacgcg gactgttcag agcataccgg
     3721 catcaatgtg aaggcccgca tgatctcctc gatggccatc gccggaggac ttgcaggagc
     3781 ggcaggagcg ctccatgtcc tcggctggac gcgccaggcc gcagaattag cagcgatgga
     3841 aggtctgggc ttcgacggta tcgcggtcgc tctgatcggc ggcacctcgc ctattgggtg
     3901 cattccggcg ggacttctgt tcggcgccct caaatacgga ggatccaagc ttcagttcgc
     3961 cctcagagcg cctattgaag tggtgaacat cgtcatcggc tctatcatct ttttcgtcgc
     4021 catgcccaag ctgctccgga tcattcgacg gtggctggaa ccaaagaaaa ggcagggagg
     4081 tgattcccat gctgcttgag agcattgcgt tcattatcgc cacgacgctt atgtactcgg
     4141 ccccgcttat cttcaccgcc ctcggcggcg tagtttcgga gaacgcgggc gtggtcaaca
     4201 tcgggcttga aggaatgatg accctcggcg ccttcgtcgg agcggccgtc ggctacttcg
     4261 ccaacgatgc gtggatcggt tttctcgctg ccggagcggc cggaggagcg ttggcgttct
     4321 tccacgccct cgcatgcgtg acgttcctcg ccgaccacat tgtctcgggt atcgcgatca
     4381 acttcatcgg ccccggtctc tccctttttc tctctcgcta cttcttcgac ggggcgacgc
     4441 agacgctctc actctctctc gacgcgaaaa ttccccgacc gttgaacggg gttttcgagc
     4501 agaacggctt ctttgatatg gtcttcaatc agtacgcaac cgtctacctt gctctcttcc
     4561 tggtcgcctt tctctggttc attctctata gaacccgtct cggcctgaga attcgcgcag
     4621 tcggcgagca ccccaagcgg cggatacctt gggtata
//
LOCUS       DAOPKU010000201         4635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20244_length_4635_cov_4.427948, whole genome shotgun sequence.
ACCESSION   DAOPKU010000201 DAOPKU010000000
VERSION     DAOPKU010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20244_length_4635_cov_4.427948"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PK849_07360"
     CDS             <1..588
                     /locus_tag="PK849_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE65980.1"
                     /translation="VLGFGEALRVLLENLEITNGARGLPGIDNFTTLPIVFLCLAGGI
                     FVARNFLRSRWGAACIAIREDPIAAETAGVRLFRTRLLSLVVSAVYCGLSGGLLAHFL
                     SFIQPQMFTLVQSTQLLAAVIAGGMGSISGPLIASFVFIALPEALRVAQMWRLVAYGL
                     LLVLIMVYRPQGLMGYSEVASFFRRKSGPEGGRRS"
     gene            585..1370
                     /locus_tag="PK849_07365"
     CDS             585..1370
                     /locus_tag="PK849_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65981.1"
                     /translation="MSESKVLLSVAGLTKDFGGIRAVDSFSFEVREGSITGIIGPNGA
                     GKTTVFNLVTGLYKPTSGTIRFLGEEIGGNRPDVIVRHGIARTFQNIRLFNRRTCLEN
                     VLTPLLQRERCSFLGAMLGLPSVRRMERALHDKAHSLLSALGLDAFASFEASTLPYGL
                     QRKLEIARALATEPSLLLLDEPAAGMNPEETHSLANLIAEVREKFNVTILLIEHHMDL
                     VMNICSPIVVMNFGALLATGTPEAVRANPEVVKAYLGERKEKR"
     gene            1367..2074
                     /locus_tag="PK849_07370"
     CDS             1367..2074
                     /locus_tag="PK849_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE65982.1"
                     /translation="MSALRVENLNVHYGTIHAVQGVSFSLEEREIVSIVGSNGAGKST
                     VMWTLAGVVERSGGSVDIFGESLPTKPHEIVARGLALVPERRRLFSALTVSENLIMGA
                     YRRKADASVEEDMERCFQLFPILKKRLNQRSGTLSGGEQQMLAISRALMSKPRILLLD
                     EPSLGLAPLLVETLMETILRIRSEGMTVLLVEQNATQALEISDRGFILEAGRFQKSGP
                     GKELAGDPEIRKAYLGG"
     gene            complement(2226..3116)
                     /gene="metF"
                     /locus_tag="PK849_07375"
     CDS             complement(2226..3116)
                     /gene="metF"
                     /locus_tag="PK849_07375"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HPE65983.1"
                     /translation="MLIRDLLSRTRPAISFEIFPPKPETPIETVFKTVAGLKELSPDF
                     ISVTYGAGGSSRDRTVEIASAVHNRWGLVSLAHLTCMGSSPAEVEKVMEDLEQEGVCN
                     ILAMRGDPRPDGGENHFQHASDLIRHIRRGHFRDFSLGAAAYPEGHPESPSLEEDLLH
                     LKEKVDAGADFLITQLFFDNEIFFRFMDRIRALGIAVPVLAGIMPVLNARQIGRIVSL
                     CGASIPPRFARIMSRYEHSPEALSEAGIAYATEQIIDLLSWGADGIHLYTMNRPDVAK
                     RIMESIATVRRTLNGSSSGV"
     gene            complement(3137..>4635)
                     /locus_tag="PK849_07380"
     CDS             complement(3137..>4635)
                     /locus_tag="PK849_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPE65984.1"
                     /translation="RIIGERINVSRKSPLRNEVERFVWTTLREEARLQTEAGAHVIDV
                     NVGLPTIDQQAAMGAAVSAAGQSTALPLSIDSDSALVLESGLGAVTGIPLINSFTARE
                     DSLFPGLGLAKRHGAAAVVLPIDENGLPESEEARKRVILKILESADSLGFPREGLIVD
                     GLTLAVGADHAAPAVTLSALTFLREQGIASVLGVSNVSHGMPARSLLNRTFLAMAVAA
                     GLDAAIMNPLDHSMMETLAAAETLAGRDAGGRRFLGAAASFSAPSDAPRPAAHAAGQS
                     AGDETLAVDPRFLPLALSILRGDAQQAEANAQELLGSGTPPLDVVNKGVIPALEEVGK
                     RYDSGAFFLPQLISSAETAQHVCELAMELVERSGGIASKGRVLLATVEGDLHDLGKNV
                     VGTVLKSHGFTVVDLGKDVPSARILEEAEKGAFDFVGLSALMTSTMKRMAETTTEIVR
                     RLPGVFVIVGGASVSEDFARTIGAHGYAPDAVSSVRLVESLMASRKTQ"
BASE COUNT          867 a         1378 c         1474 g          916 t
ORIGIN      
        1 gtgctcggct tcggcgaagc cctcagggtg cttctggaaa accttgagat cacgaacggc
       61 gcgcgcgggc ttccggggat cgacaacttc accacgctgc ccattgtttt tctctgtctt
      121 gccggaggga tcttcgtcgc ccgcaacttc cttcgctccc gctggggagc ggcgtgcatc
      181 gccatccgcg aggaccccat cgcagccgag acggcgggcg tcaggctctt cagaacccgc
      241 ctgctctccc tcgtcgtgag cgccgtctat tgcggtctct cgggagggtt gctcgcgcac
      301 tttctctcgt tcatccagcc ccagatgttc acgctggtgc agtcgacgca gcttctcgcg
      361 gcggtcatcg ccgggggtat ggggagtatc tcggggccgt tgatcgcctc cttcgtgttc
      421 atcgcgctcc ccgaggcgct cagagtggcg cagatgtggc gtcttgtggc atacggcctg
      481 ctccttgtgc tcatcatggt ctatcggccc cagggactga tggggtactc cgaggtcgct
      541 tcattcttcc ggagaaaatc cggcccggaa ggggggcggc gttcatgagc gaatcgaaag
      601 tattgctctc tgttgcgggc ctgacaaagg actttggcgg aatacgcgcc gtggactcct
      661 tctctttcga ggtccgagag ggctcgatca caggtatcat cggtccgaac ggcgcgggga
      721 agacgacggt cttcaacctc gtcacaggat tgtacaaacc gacttcggga acgatccgtt
      781 tcctaggaga ggagatcggc ggcaaccgcc cggatgtcat cgtccgccac gggatcgcaa
      841 ggacgttcca gaatatacgt cttttcaacc ggcggacctg ccttgaaaac gtactcacgc
      901 cgctgctcca gagggagcgg tgcagcttcc tcggcgcgat gctcggattg ccgtccgtcc
      961 gcagaatgga acgcgcgctt cacgacaagg cgcattccct gctgtccgca ttaggactcg
     1021 acgcgttcgc ttccttcgag gcgagcacgc tcccgtacgg cctgcagcgt aagcttgaaa
     1081 tcgcgcgcgc gctcgccacg gagccgtcgc ttctgctgct cgacgaaccc gcggccggta
     1141 tgaacccgga agagacgcac agtctcgcga accttatcgc ggaggtgcgc gagaagttca
     1201 atgtgacgat tcttctcatc gagcatcaca tggatctcgt gatgaacatc tgttcgccca
     1261 tcgtggtgat gaacttcggc gcgctgctgg cgacaggaac gcccgaagcg gtacgggcga
     1321 acccggaagt cgtcaaggct tatctgggtg aaaggaaaga aaaaagatga gcgctctgcg
     1381 ggttgagaat ctgaacgtcc attacggaac tatccatgca gtgcagggag tttcgttctc
     1441 ccttgaagag cgggagatcg tctcgatcgt tgggtcgaac ggcgcgggga agtcgaccgt
     1501 catgtggacg ctcgccggag tggtggaacg ctccggcggt tcggtggaca tcttcggtga
     1561 atccctcccg acgaagccgc atgagatcgt cgcgcgaggc cttgcgcttg tcccggaacg
     1621 tcgccgtctc ttctcggcgc ttactgtgtc ggagaatctg atcatggggg cgtaccggcg
     1681 gaaggccgat gcgagcgtcg aagaggatat ggagcgctgc ttccagctct tcccgatcct
     1741 gaagaaacgg ctgaatcagc gctcgggaac gctttcgggg ggagagcagc agatgctcgc
     1801 catctcgagg gccttgatga gcaagccgag aattctcctt ctggacgaac cgtccctcgg
     1861 tctcgcccct cttctcgtgg agacgctgat ggagactatt ctcaggattc gtagcgaggg
     1921 aatgaccgtc ctgctggtgg agcagaacgc gacccaggct cttgagatca gcgaccgagg
     1981 gtttatcctg gaggcggggc gtttccagaa aagcgggcct ggcaaagaac tcgcaggaga
     2041 cccggagatc cggaaggcct atctcggcgg ctaaacctga gggactccga cgactgcgga
     2101 aaaaacgcgg gattgcaggt gtctgtttcg ggcagaaccc tggtattgaa tgctgaggga
     2161 ttgattcaag aggcggtaag cctcggatca tccctcagca ttgttttgta ctctctcagg
     2221 attcgtcaga ctccggagga actcccgttg agcgttcttc gcaccgtggc gatgctctcc
     2281 atgatgcgct tcgccacgtc ggggcggttc atcgtgtaaa ggtggatacc gtcggcgccc
     2341 caggagagaa gatcgatgat ctgctccgtc gcgtaggcga tccccgcctc tgaaagagct
     2401 tcgggagagt gctcgtagcg ggacatgatg cgggcaaaac gcggcgggat cgacgcgccg
     2461 cagagggaga cgatcctccc gatctgccgt gcgttgagca ccggcatgat tcctgccagc
     2521 accggaacag cgattcccag ggcgcgtatt ctgtccatga aacggaagaa gatttcattg
     2581 tcgaagaaga gctgcgtgat cagaaagtcc gctcccgcat cgactttctc cttcaggtgg
     2641 aggagatcct cctcaagtga aggagattcc gggtgcccct cggggtaggc cgcagcgccg
     2701 agggagaagt ctcgaaaatg tccgcgccgg atatgacgga taaggtcgga tgcgtgctga
     2761 aagtggttct ctccgccatc cgggcgggga tctccccgca ttgcgagtat attgcagaca
     2821 ccttcctgct ccagatcctc cataactttt tcaacctccg ccggggaaga acccatgcag
     2881 gtgagatgcg caagagaaac aagcccccac cggttatgca ccgcggaggc tatctccacg
     2941 gtcctgtcgc ggctgcttcc tccggcgccg tacgtaacgc tgatgaaatc gggagagagc
     3001 tccttcagcc cggccacggt cttgaatacc gtctctatag gcgtttccgg tttcggagga
     3061 aatatctcga aggagatcgc cggacgcgtt cgcgagagaa gatcacggat aagcatagcg
     3121 cgtgcatacc tcctttttac tgagtttttc ggctcgccat gagcgattcg acaagccgga
     3181 ccgagcttac cgcatcggga gcgtagccgt gcgcgccgat ggtccgggcg aagtcctcgc
     3241 tcacggaagc gccgccgacg atgacgaaaa cccccggaag gcggcgtacg atttccgtcg
     3301 tcgtctccgc cattcttttc atcgtcgagg tcatcagtgc ggagagccct acgaaatcga
     3361 acgctccctt ttcggcctct tcgagaatac gtgcggaggg gacgtccttc cccaggtcga
     3421 ccactgtgaa accgtggctc ttgagaaccg tgccgacgac gttcttcccc aggtcgtgca
     3481 ggtctccttc aacagtggcg aggagcactc tccccttcga cgcgatccca cccgatcgct
     3541 ccacgagctc catagcgagt tcgcagacat gctgcgcggt ctccgccgac gagataagct
     3601 ggggaaggaa gaaggcgccg ctgtcgtagc gcttcccgac ctcttcaagc gcgggaatga
     3661 ctcctttgtt caccacgtcg agcgggggtg ttcctgagcc gagaagttcc tgcgcattcg
     3721 cctccgcctg ctgcgcgtcg ccgcgaagta tcgaaagggc gagcggcagg aaccggggat
     3781 cgacggcgag cgtttcgtct cctgcggact gacccgcggc gtgtgccgca ggacggggcg
     3841 cgtcggaagg cgcggagaac gaagcggccg caccgaggaa gcgacgccct cccgcatcgc
     3901 gtcctgcaag cgtctccgct gcggcgaggg tctccatcat ggagtggtcg agcgggttca
     3961 taatggcggc gtccagcccc gcggcgacgg ccatcgcgag gaacgtcctg ttgaggagcg
     4021 aacgggcggg catgccgtgc gagacgttgc tcacacccag gacggaggcg atcccctgct
     4081 ctctgaggaa ggtcagggcg gagagcgtga ccgccggagc ggcgtgatcc gcgccgacgg
     4141 caagcgtcag tccgtcgacg atgaggcctt cacgcggaaa tcccagagag tccgcgcttt
     4201 cgagaatctt aaggatgact cttttacggg cctcttcgct ttcgggaaga ccgttctcgt
     4261 cgatcggaag cacgaccgcc gcagcgccgt ggcgctttgc cagtcccaag ccggggaaga
     4321 gggagtcctc tctggcggtg aaggagttga tgagggggat accggtaacg gccccgagcc
     4381 ctgattcaag caccaaggcg ctgtcgctgt cgatggagag cggcaacgct gtggactggc
     4441 ctgcggcgga gactgcggcg cccattgctg cttgctgatc gatcgtcggc aggccgacgt
     4501 tcacgtcgat gacatgcgcg cccgcttccg tctgcagccg cgcctcctcg cggagcgtcg
     4561 tccagacgaa gcgttcgacc tcgttcctga gcggggattt gcgcgagacg ttgatccgct
     4621 cgccgatgat gcgga
//
LOCUS       DAOPKU010000202         4627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20300_length_4627_cov_4.168854, whole genome shotgun sequence.
ACCESSION   DAOPKU010000202 DAOPKU010000000
VERSION     DAOPKU010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20300_length_4627_cov_4.168854"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..820
                     /locus_tag="PK849_07385"
     CDS             170..820
                     /locus_tag="PK849_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE65985.1"
                     /translation="MKIRVLMIDDHTLFLAGLEDILAREDDIEVVGVAGSCREGVSLA
                     MEKRPDVIVLDLAMPGTSGVEAARILAEALPGVRMAALTMHLDRQMVFEALKSGMTGY
                     ILKEATPEEFLFALRVVARGETWLSPKVAAIVANDFVRGAAQEAPSLPVLSDREREVL
                     HLLVKGAGTREIASRLHISKSTVDTHRRNILDKLGCENVTCLTRYALRHGLVDLDE"
     gene            884..1216
                     /locus_tag="PK849_07390"
     CDS             884..1216
                     /locus_tag="PK849_07390"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPE65986.1"
                     /translation="MAYSVEYFNDRVRAAIEAWPVGILADYARILEMLMEFGPEVGMP
                     YSRPMGDGLFELRPRGREGIGRALYCYMRGRRLLILHAFVKKTGSTPQKDLNRARDRL
                     KEVRHDWM"
     gene            1203..1553
                     /locus_tag="PK849_07395"
     CDS             1203..1553
                     /locus_tag="PK849_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPE65987.1"
                     /translation="MTGCEYKPVPHNHDEFLAAMSEREGFTSAYAALEDEYRFVRELL
                     AARIRAGLTQEEVAESMGTSKSAVSRLEAGGKHSPSVQTLRKYAKAVGCDVEIRLVEK
                     PSRCASPANNSSAM"
     gene            complement(1754..2629)
                     /locus_tag="PK849_07400"
     CDS             complement(1754..2629)
                     /locus_tag="PK849_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPE65988.1"
                     /translation="MNEFSFFATQGPLSRPGAHAELFDSLPADIGELCAVVQGVTVHP
                     FWAERYGVTLAAERGAEVQLRTMEKRLARTLELDSRPLTVPRPLELKTVGNCRDHTLL
                     LVSMLRSQGVPARARCGFGTYFLPGHFEDHWVAEYWNESEKRWILVDAQLDAFQRGVL
                     NVSFDPLDVPRDRFIGGGAAWRMCREGVADPNAFGIFDMRGMGFVGSNLVRDVAALNK
                     VELLPWDCWGMMLERDTDDSDLALLDRLAELTEGDVPDFERVRELYESEPRLKAGATI
                     QSFVQGEMKVVELEL"
     gene            2949..>3194
                     /locus_tag="PK849_07405"
     CDS             2949..>3194
                     /locus_tag="PK849_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPE65989.1"
                     /translation="MEFAGRTALVTGAGTGNGEAIAERLFTGGASVGLVSRRLESVDA
                     VCRRIDPEGERTLAIEADVRDPKAMEAAVQRIVERFGS"
     assembly_gap    3195..3294
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3316..4095)
                     /locus_tag="PK849_07410"
     CDS             complement(3316..4095)
                     /locus_tag="PK849_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE65990.1"
                     /translation="MGRFFTAFLLLVLLGSTACVSVASAGETVVRITNGDWPPFLGEH
                     LPGNGAISQVVRESFALKGIETRYGFFPWARALALAAEGKWDASVGWAFSEERARDFF
                     FSDALVDLPVLLFSLNEKKVEWKEWQDLAGLLIGVTRGYHYGNEFEKASEEGLFRTDP
                     SPDDETSLRKLLGKRVDAVAVDRYVGIELINRGFALEADRFWYCETPVRSAPYSLLFS
                     KKAPNAEELLAAFNEGLAELKASGRYQEILDDAASGKYSTP"
     gene            4433..>4627
                     /locus_tag="PK849_07415"
     CDS             4433..>4627
                     /locus_tag="PK849_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPE65991.1"
                     /translation="MGLKRGMVRVAVVQAGSVVMDKEGTLEKTERLAIEASEKGAKIV
                     LFPEAFIPCYPRGLSFGAVVG"
BASE COUNT          953 a         1294 c         1382 g          898 t
ORIGIN      
        1 tctcctccaa ggcgccgaag cactggggga agtggggcgg catgggcctt ttcagcatcc
       61 gtgagaaact tcactccgtc ggcggagaga ttcgcgtctt ctcggagaag gggaaaggga
      121 gcaccgtcgc gctcgtcgcg ccgctcatgt tcgagggagg aaagacgtca tgaagatccg
      181 cgtgctgatg atcgacgatc atacgctctt cctggccggg ttggaggaca ttctcgcccg
      241 cgaggacgac atcgaagtgg tcggcgtcgc cgggagctgc cgtgagggcg tgtcgctggc
      301 gatggagaag cgccccgacg tgatcgtcct cgacctcgcg atgccgggga ccagcggcgt
      361 ggaggcggcg cgcatcctcg ccgaggcgct gccgggcgtc cgcatggcgg cgctcacgat
      421 gcacctcgac cgccagatgg tcttcgaggc gctcaaaagc ggcatgaccg gctacatttt
      481 gaaagaggcc actccggagg agttcctctt cgcgctccgc gtggtggcgc gcggggagac
      541 ctggctcagc ccgaaggtgg ccgccatcgt ggcgaacgac ttcgtccgcg gcgccgctca
      601 ggaagcgccg agccttcctg tcctctcgga tcgcgagcgc gaagtactcc atctcctcgt
      661 caaaggggcg ggaacgcgtg agatagcgtc tcgtctgcac atcagcaaga gcaccgtgga
      721 cacccaccgg cgcaacatcc tcgataagct gggctgcgaa aacgtcacct gcctcacccg
      781 ctacgccctg cgccacggcc tggtcgacct ggacgagtga ggggcaaggt ttttcaagtc
      841 actctgcatt gacgtgtgtt ctcatatttg ataacatgcg gacatggctt attccgtcga
      901 atacttcaat gatcgagtcc gtgcagcgat agaagcgtgg cccgtcggta ttcttgccga
      961 ctatgcgcgg attcttgaga tgcttatgga gttcggtccg gaagtgggga tgccgtattc
     1021 ccgtccgatg ggagacggtc ttttcgaact gcgcccccga ggacgcgaag ggatcggacg
     1081 tgcgttgtac tgctatatga gaggaaggcg tctgctcata ctccacgcct ttgtaaagaa
     1141 aacgggctcc actccgcaga aggatttgaa tagagcccgc gatagattga aggaggtgcg
     1201 tcatgactgg atgtgaatat aaaccggttc ctcataatca cgatgagttt ctggccgcga
     1261 tgtcggaaag agaaggtttt acctccgcgt atgccgcgct ggaggatgaa taccgatttg
     1321 tccgcgaact tttggcggcc aggatacgcg caggtctcac acaagaagag gttgcggagt
     1381 ccatgggaac aagtaaaagc gcagtttccc gcctggaagc ggggggaaag cattctcctt
     1441 ccgtccagac gctgaggaag tatgcaaaag cagtagggtg cgacgtcgaa attcgtctgg
     1501 ttgaaaaacc gtctcgttgc gcttcgcctg caaataactc ttcggcgatg tgaggttttt
     1561 gaggctttcg gcggagacgg agagcggcgc atggagtctc gggaatgctt cgaggcaaga
     1621 agactcccca aaaaaaccgc tttccgtccg tctctcactc ctaaggatgt acttgtcacc
     1681 gcatcgtccg ccggataaag gattcgcgcc tgcgtgaaga agctccctcc ggtcttccta
     1741 cgatggatgc gtttcagagt tccagctcga ccactttcat ttcgccctgc acgaaacttt
     1801 ggatcgtcgc gccggccttc aggcgcggtt ccgactcgta gagttcgcgc acgcgctcga
     1861 agtccggaac gtcgccctcc gtcagttcgg ccagacgatc gagcagcgcc aggtcggagt
     1921 cgtccgtgtc gcgttcgagc atcatccccc aacagtccca cggcagcagt tcgaccttgt
     1981 tgagcgccgc gacgtcgcgg acgagattgc ttcccacgaa acccattccc cgcatgtcga
     2041 agatgccgaa ggcgttcggg tccgcaaccc cctcgcggca catcctccag gccgcgccgc
     2101 cgccgatgaa gcggtcccgg ggcacgtcca gcggatcgaa tgaaacgttc agcacgccgc
     2161 gctggaaagc gtcgagctgc gcgtcgacca gtatccagcg tttctcggat tcgttccagt
     2221 attcggcgac ccagtgatcc tcgaagtgtc cgggcaggaa gtacgtcccg aatccgcagc
     2281 gcgcacgggc cgggacccct tgactgcgca gcatggacac cagcagcagc gtgtggtcgc
     2341 ggcagttgcc gaccgtcttc aattccagcg gccgaggaac ggtcagtggg cgcgaatcga
     2401 gttccagagt gcgggccaga cgtttctcca tggtacggag ctgcacctcg gcgcccctct
     2461 cggcggcgag cgtcaccccg taccgttcgg cccagaaggg gtgaacggtg acgccctgga
     2521 cgaccgcaca caactccccg atgtccgcag ggagagaatc gaaaagttca gcgtgcgctc
     2581 cgggcctgct gagaggccct tgagtcgcga aaaacgagaa ttcgttcata ggtttcctcc
     2641 ttaaacagag agcggcgtac cccgcttcga acgccgcagc gtccggcgga tcgcgtctaa
     2701 cagtattcgg gaacggggat cgactcccgt ccgacggtat gtattgtatc agttgcctcg
     2761 gcccggcgcc tcggtgcaga caccacccgg ccctctttca aaaacggagg gtgaggtctc
     2821 cgagaaggcg ccattcgtgg tactattttc taaagaagtt ttattgtcaa gaagcagaat
     2881 gattgggagt catggcataa ccatcgtgca acaatgagga aacacctgac gcagcctgga
     2941 ggtgcgatat ggaatttgca ggaagaacag cgctggttac cggcgccgga acagggaacg
     3001 gcgaagcaat agccgagcgg ctcttcaccg gcggagcgtc ggtcgggctg gtgagtcgcc
     3061 gtcttgaatc agtggacgca gtctgccggc ggattgaccc tgaaggtgaa cggacgctgg
     3121 ctatcgaggc cgatgtgcgc gatcccaagg cgatggaggc tgcggtgcaa cgcatcgtcg
     3181 aacgcttcgg ctcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggctc
     3301 gtacgggcga aaacgtcacg gagtcgaata cttgccggac gccgcgtcgt ccagtatctc
     3361 ctggtatcgg ccgcttgcct tcagctcggc gagcccctca ttgaatgctg ccagcaattc
     3421 ctcggcgttc ggcgcttttt tcgaaaaaag cagagagtac ggcgccgagc ggacgggggt
     3481 ttcgcagtac cagaaccggt cggcttccag ggcaaatccg cggttgatca gctcgatacc
     3541 tacatatctg tcgactgcga cggcatccac tctttttccg agcagcttcc tgaggctcgt
     3601 ctcatcgtca ggagatggat cggttcggaa gagaccttct tcactcgctt tttcgaactc
     3661 gttcccgtag tgataccccc gcgtaactcc gatcaagagc cctgcaaggt cctgccactc
     3721 cttccactcg accttctttt cgttcaggga gaagagaagc accggcagat cgacaagcgc
     3781 gtcgctgaag aaaaagtccc gcgctcgttc ttccgaaaaa gcccacccga cggaagcgtc
     3841 ccacttcccc tccgcggcga gagcaagagc gcgcgcccac gggaagaatc cgtacctcgt
     3901 ttcgatgccc ttaagggcga aggattccct cacaacctgc gagatcgctc cgtttccggg
     3961 aagatgctcc cccagaaacg gaggccagtc gccgttcgta atacggacga ccgtttctcc
     4021 ggccgaagca acggagacgc aggcagtcga accaagaaga acgagaagaa gaaacgctgt
     4081 gaaaaatcgc cccatgagca acacctcccg cgtgatggta taatgcgaaa ttcgcaaatg
     4141 gaaaatagca ctaaaaaata caatttgtat tgatttcttg aaattgtagg ccgaaatgtt
     4201 atgggtgtca agcacccctt cagcgggctc gtttttttcg gaagaaattt aagacatgat
     4261 gaccagtcga gcggagcgtt ctctttgtgt aataatagca agatcgcggg gttttcgcga
     4321 aacgaaacac cggacgacgc gtcttttcaa tgtgtttagg gcggcgcttt gcttcatttg
     4381 gccgtgcgga gacgaaccgg aaatttgccg gaagaaacgg gggaagagca caatgggtct
     4441 gaaacgcgga atggtacggg tagcggtggt gcaggcgggg tccgtggtca tggacaagga
     4501 aggaactctg gagaagacgg aacggctcgc gatcgaagct tccgagaagg gggcgaagat
     4561 cgtgcttttt cccgaggcgt tcattccgtg ctatccgcgc gggctctcct ttggcgccgt
     4621 cgtgggc
//
LOCUS       DAOPKU010000203         4623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20328_length_4623_cov_5.036996, whole genome shotgun sequence.
ACCESSION   DAOPKU010000203 DAOPKU010000000
VERSION     DAOPKU010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20328_length_4623_cov_5.036996"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PK849_07420"
     CDS             <1..511
                     /locus_tag="PK849_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65992.1"
                     /translation="KEPLLRRTALLASFRRKLSFPDLEGASSVRAILEGWRWPKRVCS
                     GCAALVCRRAAPAANMSRAEMADLLLAHGEPFWESVFALFGALCRVHVPRRRMALFRE
                     RFGRLAARLRTEEALIPSGDELMERYALSSGPEVGRLLKALRTEHLLKGFASREEAME
                     RVASLMNIR"
     gene            608..1867
                     /locus_tag="PK849_07425"
     CDS             608..1867
                     /locus_tag="PK849_07425"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPE65993.1"
                     /translation="MGLLHVDQLQAGMVLEKNLLAFNGRFLLPRGIVLTEKHIQTMKV
                     WGVVEASVEGDLPAEPLKDAAASMPPEVVQEAGRSLETLFPSTPSTSPMKDFYSVAME
                     RTTAALMRDRSQRGAQAQQPRSPARRLSSNIPNLSVTASDLVSRELQLASLPDVYYRI
                     MEVINSTRSSASHIAEAVGKDTSLLSRLLRLVNSAFYGFPSKIETVTRAVAIVGTREL
                     STLALGVAAVQYFNDIPSEYVDMKSFWRHSVACGVYARLMASEKIGVSEERLFVSGLL
                     HDLGKLVLFRQVPLSARNAIELALAKKIPLCEAEREILGFDHAYLGALLLREWKIPSP
                     LENAVRFHHSPLSSSSPLDPALLASADALAMATGFSGSGSVVMPQISPEIWAETGLSA
                     AVFQPVILQGGRQIEDITNAFLGNGGA"
     gene            1869..3527
                     /locus_tag="PK849_07430"
     CDS             1869..3527
                     /locus_tag="PK849_07430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE65994.1"
                     /translation="MLRRNRISFMEERLRRLEEQNAYLWQEKAFGIEALDAAASLGHF
                     DTSMNKLEDPLPILRETVKRVQGLIPLKAAVIYLVGDDSSFYPAYCSPPDALPELEKR
                     VATLIEDRTFVWAIKRRKPVLTEFKEKRAPLLLHVLSTISRTRGMLVGVMDKGWEEIS
                     DYALSLLSVALTASAGALESFELYRNLRSLNRDLSSKMAQLEDSRNELERYHVVLEEQ
                     VETRTRELAQANDEMKMEIAERMRAEEELRSSESMLKHALEGVGEGIWMWDVASGEVD
                     LSPLSMEILGCAADVAACPPEMRWMEGWERLIHPDDVEKVRYKKQKCLAGDTQGYRVE
                     YRVPGGDGTVRWILERARVVLRDDARKPLRIAGTHSDVTERRELEDQIRFQATHDYLT
                     GLPNRYLFYDRFSQAIAYARRKKRKVSLLFIDLDNFKVVNDTYGHDVGDDLLKEAAER
                     LRKVFREMDTICRLGGDEFTVILPETGDRAEVQRIAERLLEFFDTPFPMNSGSIRLQI
                     SVGISIFPDDGDDIEMLLKKADLAMYRTKKHQRSAFAFAADVHD"
     gene            3689..>4623
                     /gene="larA"
                     /locus_tag="PK849_07435"
     CDS             3689..>4623
                     /gene="larA"
                     /locus_tag="PK849_07435"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPE65995.1"
                     /translation="MRVSIPYGTSALSCDIPDSRLKAVLVSSLHELKADAPERQIVRK
                     SLENPIGSLGLRELAEGKKNVVIISSDHTRPVPSAIIMPLLLEEVRAGNPDAEITILV
                     STGGHRATTPGELLKKYGTEIVKNERIVVHDSHDDASLVHVGTLPSGGDLILNKLAVE
                     ADLLVAEGFIEPHFFAGFSGGRKSVLPGVASYTTVMANHCAEFIAHERARTGILEGNP
                     IHGDMVFAARKVGLAFVCNVVIDAEKHIVAAFSGDMEAAHEKGCEFVAKYGRVKAVPA
                     DIVITSNAGYPLDQNLYQAVKSMTAGEASCRKGGV"
BASE COUNT          931 a         1325 c         1403 g          964 t
ORIGIN      
        1 gaaagaaccc cttctcagac ggacggcgct tctcgcctcg ttccggagga agctctcctt
       61 ccccgacctc gaaggcgcct cctcggtccg cgcaatactg gaaggatggc gctggccgaa
      121 acgggtgtgc tcgggatgcg cggcgcttgt gtgcaggagg gccgcccctg cggcgaacat
      181 gtcgcgggcg gagatggccg atctgcttct tgctcacggc gagcccttct gggagtcggt
      241 ttttgctctt ttcggcgctc tctgccgagt gcatgtcccg cgcaggcgca tggctctttt
      301 ccgggagcgt ttcggccgcc tcgccgctcg cctccggacg gaagaagcgc tcattccctc
      361 cggagatgag ctgatggagc ggtatgcgct ctcttcaggt ccggaggtgg ggagactctt
      421 gaaggctctt cggaccgaac atcttctcaa gggattcgct tcgagggagg aggcgatgga
      481 gagagtcgct tcgttgatga atatacgatg aagagaggaa ctcccctctc tgttttccgc
      541 tctcttcgtg ttacaatcag aagaaacata tttgtatctg cagagggtat agagggagtc
      601 gattttcatg ggtctgcttc atgtcgatca gttgcaagcg ggcatggtac tggagaagaa
      661 tctgctggct ttcaacggcc gctttcttct cccaaggggt attgtgctta cggagaagca
      721 tattcagacg atgaaggtct ggggggttgt agaggcgtcc gttgagggag atctccctgc
      781 ggagcctctg aaggatgccg cagcctcaat gcctcccgag gttgtgcagg aggccgggcg
      841 ctcgctggag acgctcttcc cctccactcc gtcgacttcg ccgatgaaag atttctactc
      901 tgtcgccatg gaacggacaa ccgcagccct gatgcgcgac cggtcgcagc gcggggcgca
      961 ggcgcaacag ccccgcagcc ccgcgcgccg gttgtcctcg aatatcccca atctgtctgt
     1021 gacagcctcc gatctcgtct ccagggagct gcagcttgcc tctctccccg atgtgtacta
     1081 tcggatcatg gaggtgatca attcgacccg cagctccgcc tcccatatag ccgaggccgt
     1141 cgggaaggat accagtcttc tttcccggct tcttcgtctt gtcaacagcg cgttctacgg
     1201 cttcccctcc aagatagaga ccgtgacgcg ggccgttgcg atcgtcggaa cccgcgagct
     1261 ttcgacgttg gcgctcgggg ttgcggccgt ccagtacttc aacgacatcc cctcggaata
     1321 tgtggacatg aagagcttct ggaggcactc cgtggcctgc ggcgtctacg cccgtctgat
     1381 ggcctcggag aagatcggcg tttccgagga acgtctcttc gtctccggac tgctgcacga
     1441 cctgggaaag ctcgtgctct tccgtcaggt gcctctttcg gcgcggaacg ccatcgaact
     1501 ggctcttgcc aagaaaattc ccctctgtga agcggagcgg gagatcctcg gcttcgacca
     1561 cgcctatctc ggggcgttgc tgctgcggga gtggaaaatt ccctcgcctc tcgaaaacgc
     1621 cgtacggttt catcactccc cgctttcctc ttcctctcct cttgatcctg cgcttctggc
     1681 gtcggcggat gcgctcgcca tggcgacagg attcagcggg agcggctcgg tcgtgatgcc
     1741 ccagatctcg ccggagatat gggcggagac ggggctttca gcggccgtct tccagccggt
     1801 gatccttcag ggaggccgcc agatagagga cattacgaac gcttttctcg gaaacggagg
     1861 cgcatagcat gctccgtcgc aatcgcatct cttttatgga ggaacgcctc cgccgcctgg
     1921 aggaacagaa cgcatacctc tggcaggaaa aagcattcgg catcgaggcg cttgatgccg
     1981 ctgcttcgtt ggggcacttc gacaccagca tgaacaagct cgaagacccg ctgccgattc
     2041 tccgggagac tgtcaagcga gtgcaggggc ttatccctct gaaagcggcc gtgatatatc
     2101 tcgtcggcga cgactcttca ttctaccccg cgtactgctc cccgcccgat gcgcttcccg
     2161 agctggagaa gcgtgttgct actctcatcg aggacagaac gttcgtctgg gcgatcaaga
     2221 ggcgcaaacc tgtcctcacc gaattcaagg aaaagcgcgc tccgctgctg ctccacgtgc
     2281 tctccaccat ctcaaggacg cggggcatgc tggtaggggt catggacaag gggtgggagg
     2341 agatctcgga ctacgccctc tctcttctct ccgtcgctct gactgcaagc gcgggagctc
     2401 tggagagctt tgagctgtac cggaatcttc gcagtctcaa cagagatctc tcctcgaaga
     2461 tggcgcagct ggaggactcg cggaacgagc ttgaacggta tcacgtggtt ctggaggaac
     2521 aggtggagac tcgtaccaga gagctggcgc aggcgaacga cgagatgaag atggagatcg
     2581 ccgaacggat gcgcgccgag gaagagcttc gttccagcga gagtatgctc aaacacgccc
     2641 ttgagggtgt cggcgaagga atatggatgt gggacgtcgc ttcgggagag gtcgatcttt
     2701 cgccgctttc gatggagatt ctcggatgcg ccgccgacgt cgccgcctgc ccgccggaga
     2761 tgcggtggat ggaggggtgg gaacggctca ttcacccgga tgatgtggag aaggtccggt
     2821 acaagaagca gaagtgcctt gccggagata cgcaggggta cagagtcgag taccgcgtgc
     2881 cgggcggcga cgggactgtc aggtggattt tggagcgcgc ccgcgttgtt ctcagggacg
     2941 acgcgagaaa accgcttcgg atagccggca cccactccga tgtcaccgaa cggcgggagc
     3001 tggaggacca gatccgcttc caggcgacgc acgactatct gaccgggctg ccgaaccgct
     3061 atctttttta tgatagattc tcccaggcga ttgcgtatgc acgccgaaaa aagagaaagg
     3121 tctcgctgct ctttattgac ctggacaatt ttaaggtcgt caacgatacg tacggtcatg
     3181 atgtcggcga cgacctgctg aaggaagccg cagaacgtct gagaaaggtc ttccgggaga
     3241 tggataccat ctgccgcctt ggcggtgacg aattcacagt gattcttccc gagacggggg
     3301 accgcgctga agtgcagcgc attgcggagc ggctcctgga gttcttcgat actcccttcc
     3361 ccatgaattc aggttcgata cggctccaga tatccgtggg gatcagcatc ttcccagatg
     3421 acggcgacga tatcgagatg ctcctgaaaa aggcggacct cgccatgtac cgcacgaaga
     3481 aacaccaaag aagcgcgttt gcctttgcgg cagacgtgca tgattgacca gcctttcacc
     3541 ggaaactcaa aaaggaaaac gccctaataa agtcaattct tgcatgcgcc tcttgacctc
     3601 ttgttcaatt gtcttaataa tgtttattgg aattttcaaa aagtagcgtg cggcagcaga
     3661 atgatttttc aacaggaggc gagtgcacat gcgagtatcc attccgtacg gaacgtcggc
     3721 tctttcctgc gatatacccg acagccgttt gaaagcggtt ctcgtttcga gtcttcacga
     3781 actgaaagcc gatgcgccgg agcgccaaat cgtcagaaaa tccctcgaaa acccgatcgg
     3841 atccctcggc ctgcgagagc tcgcggaggg aaaaaagaac gtcgtgatta tctcaagcga
     3901 ccatacccgc cccgttccaa gcgccattat catgccgctg ctcctcgaag aggtgcgggc
     3961 ggggaacccc gatgccgaaa tcaccatcct tgtctctacc ggaggacacc gcgccaccac
     4021 cccaggggag ctcctgaaga agtatgggac cgagattgtg aagaacgaac gcattgttgt
     4081 ccacgacagc catgacgatg cgagtctcgt tcacgtagga accttgcctt ccggaggcga
     4141 tttgatactc aataagctgg cggtcgaggc cgacctgctc gtcgcggagg ggttcatcga
     4201 gccgcacttc ttcgccggtt tctcgggagg ccgcaagagc gtgcttcccg gcgtggcaag
     4261 ctacacgacg gtgatggcga atcactgcgc ggagtttatt gcgcacgaaa gggccagaac
     4321 cggaattctc gagggcaacc ctatccacgg cgacatggtc ttcgccgccc ggaaggtcgg
     4381 gctggcgttc gtctgcaacg tggtcatcga cgcggagaag catatcgtgg cggccttcag
     4441 cggcgacatg gaagcggcgc acgagaaggg gtgcgagttc gtcgccaagt acggcagggt
     4501 caaggccgtt cccgccgaca ttgtcatcac gagcaacgca gggtatcctc tggaccagaa
     4561 tctctaccag gcggtgaaga gcatgaccgc cggcgaggcg tcgtgcagga agggaggcgt
     4621 tat
//
LOCUS       DAOPKU010000204         4613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20392_length_4613_cov_4.759105, whole genome shotgun sequence.
ACCESSION   DAOPKU010000204 DAOPKU010000000
VERSION     DAOPKU010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20392_length_4613_cov_4.759105"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PK849_07440"
     CDS             complement(<1..1003)
                     /locus_tag="PK849_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65996.1"
                     /translation="MKDVIGLGTILLWQWLTVLKGPLFPSLVDIPLLGPEGGFALFSL
                     ILFATFLLLARKGKDLPEAWFVRAVRLAPILMSAAWLPWGWRGASLPAPLLLFALVLP
                     PFGSAMHLASWQWRLSQAPFSRQAVSFGLACLVRSSAILLLSFAPRPLLHIVAMLLPF
                     LGSFLWNPPGAVLPPDASRGGFRLSPRLAFRVASFFIVSAMFLSILLARGNNEALASK
                     VLTDPLYTLGALGAGLALLRWFAVDLRSIYFFAQSLLVLGFMAFAALGGGRPMIPLAL
                     LQLGFGTFGAYLFTLLLYLGSRAGRSEALSVVSSIQAVNSGSVVLGMLLTRTTGFAAG
                     "
     gene            1197..2483
                     /locus_tag="PK849_07445"
     CDS             1197..2483
                     /locus_tag="PK849_07445"
                     /inference="COORDINATES: protein motif:HMM:NF018234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPE65997.1"
                     /translation="MKHRPSTLMAIFIFLLSASLLQSRAAFSTEEPLAVSDMHATAFH
                     YFFQDGDMDFHFGNLILGSSVHGGAGIGEAFYAASRIRDGNAGDWQREWAELAGRVEA
                     RGETSLAAGHLVSARDQLLRAAYYYRLSLVSMLPDNPAFKERGAKVRELFQKGGALFD
                     PPVEYFEVPFEGTSLPGYFRKAASGDRPVKTLLMIGGGETFAEDLFFYIAHQAHARGY
                     NFATVDLPGQGLLPLEGMVFRTDTQAPVKAVVDVLVKRPDVDTERLSAFGISGGGLFV
                     PQAAMFDPRIKAVAMCSAVVDARPLFATMPAALDTPEERAGWTSFHEGVVKSICWRYG
                     VPMDHPEKLIEANEGNTFDPAKVAAPALIIVGEGEYRSLEVQRQQKIALDNFPNPSKK
                     MVVTPSDEGASNHCVMENRALIGQVLFDWLDETMGE"
     gene            complement(2585..2746)
                     /locus_tag="PK849_07450"
     CDS             complement(2585..2746)
                     /locus_tag="PK849_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE65998.1"
                     /translation="MVSSKKAVFFLCVDPIDHVASWIPGDLVRYVRESKMQGEDASRA
                     HPPLEEPPL"
     gene            3004..3456
                     /gene="alaE"
                     /locus_tag="PK849_07455"
     CDS             3004..3456
                     /gene="alaE"
                     /locus_tag="PK849_07455"
                     /inference="COORDINATES: protein motif:HMM:NF018333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034639 - L-amino acid efflux
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HPE65999.1"
                     /translation="MTGTPGANNKRKQISFLIADITAMILFSTGLCMIIEIFVAGLSF
                     FQSVGARIAAIPVNLITGRPYGWFRDTLFLTLGIDRSSPVKLFFGDTLAFVVFQVPLY
                     VLVLLFAGATWKQIAVTSLSASLVFSLTGRPYGIFLDFCRRIAVKLLG"
     gene            3529..4509
                     /locus_tag="PK849_07460"
     CDS             3529..4509
                     /locus_tag="PK849_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPE66000.1"
                     /translation="MNGRMYEWRRQIQAIVDAIDECIKNHDDEALTLRRLSCGLGYSE
                     FHTTRKFKEISGMQFREYLRGRRLAFALKEVRDSGKSLLDIAFDYGFSSHEAFTRAFK
                     GTYGVAPGEYRKNPKPVVLRTKINPFDRYFLGLGEIGMMKSADDVKIYCVTIPEHKFV
                     HIKNYESNGYWDFWQKQSGIPGQDCETICGLLDSIKGKLDDDGGSESDSGSGQIMAYI
                     NDPDGRLCDWGIPRTECYGARLPLDYKGEVPPQMLLIDIPEAEYIVFEHGPFDYEQEN
                     RSVEEKIEHAMAAFDYADAGYILDTSPGRITYFYHDPGRFFKYVRPVRRK"
BASE COUNT         1074 a         1335 c         1349 g          855 t
ORIGIN      
        1 gaccggccgc gaagcctgtc gtccgggtca gcagcattcc cagaacaaca gagccgctgt
       61 ttacagcctg tatcgagctc accaccgaaa gcgcctccga tcttccggcg cgcgacccaa
      121 gatagagcag cagcgtaaag aggtaagcgc cgaacgttcc gaagccgagc tgcaacaggg
      181 caagcggaat cataggacgc ccgccgccga gcgccgcgaa cgccatgaac cccagcacca
      241 gcagggactg cgcgaaaaag tagatgctcc tcaggtcgac ggcaaaccag cggagaagcg
      301 caagtcccgc tccgagcgct cccagtgtgt acaaaggatc cgtcagcacc tttgaggcga
      361 gcgcttcgtt gtttccccgt gccagaagaa tcgacagaaa catcgccgat acgatgaaga
      421 aggaagccac acggaacgcc agccggggcg agagacggaa cccgccgcga gaagcgtcgg
      481 gcggaagaac cgctcctggg gggttccata gaaagctccc cagaaaggga aggagcatcg
      541 cgacgatatg gagcagcggt cggggagcga atgagagtaa gagaatagcg gagcttcgga
      601 ccagacatgc aagtccgaac gagactgcct ggcgcgaaaa cggcgcctgc gagagccgcc
      661 actgccagct cgccagatgc atcgccgacc cgaagggcgg aagaacaagc gcgaataaga
      721 gcagaggagc cggaagcgaa gctccccgcc agccccaagg aagccacgct gcggacataa
      781 gaatcggggc gagacgtacc gcacggacga accacgcctc gggaagatcc ttcccctttc
      841 gggcaagcaa caaaaatgtc gcgaagagaa tcagggaaaa gagcgcgaat ccgccctccg
      901 ggccaagaag agggatatcg acaagagacg ggaagagcgg ccccttcaac actgtgagcc
      961 actgccagag aagtatcgtt ccgaggccga tcacatcctt cattccacca cctccttctt
     1021 ttccaagata acaacagaaa agatatgaac agtcaagcct tgcaaagact tcttttttca
     1081 aggggggtta gagaattcac cctcccgcag gcggggttac cgatgtagaa tcttttctct
     1141 gagagttctc tccgcaagga gatggctcac tatagataca aaaaggagtg attgtcatga
     1201 agcatcgacc aagcacgctg atggccattt tcatctttct tttgagcgca agtctcttgc
     1261 agagccgcgc cgccttttcg accgaggagc cgttggccgt atccgacatg cacgccacgg
     1321 cgttccacta tttcttccag gacggcgaca tggacttcca cttcggcaac ctgattcttg
     1381 gttcgtccgt acacggaggc gccgggatcg gcgaagcgtt ttacgccgcg tcccgtattc
     1441 gggacggaaa cgcaggcgat tggcagcgcg aatgggcgga actggccggc cgagtagagg
     1501 ccaggggaga aacatccctt gccgcagggc atcttgtaag cgcccgtgat cagctcttga
     1561 gagccgccta ctactatcgc ctctcgctgg tctccatgct gccggacaat cccgccttca
     1621 aagagcgggg agccaaggtc cgggaactct tccaaaaggg cggcgcgctg ttcgatcctc
     1681 cggtggagta cttcgaagtc cccttcgagg gtacgtcgct tcccggatac ttccgaaaag
     1741 ccgcttccgg cgaccgtccc gtcaagaccc tgctcatgat cggcggcggc gagaccttcg
     1801 cggaggatct cttcttttat atcgctcacc aagcccacgc ccgaggctac aatttcgcca
     1861 cggtggacct tcccggacag ggactgcttc ctctggaggg aatggtcttc cgcacggaca
     1921 cgcaggcgcc cgtgaaggcc gttgtggacg tccttgtcaa gaggccggac gtggatacgg
     1981 agcgtctgtc cgccttcggc atcagcggag ggggactctt cgttccgcag gcggcgatgt
     2041 tcgacccgcg catcaaggcc gtcgccatgt gctccgcggt ggtcgacgcc cgccccctct
     2101 tcgccaccat gcccgccgcc ttggatactc ctgaagagcg agcgggctgg acctccttcc
     2161 acgaaggtgt ggtgaagagc atctgctggc gctacggagt gccgatggac caccccgaaa
     2221 aactgatcga ggcgaacgag ggaaacacct tcgatccggc gaaagtggcc gcgcccgctc
     2281 tgatcatcgt cggcgaggga gaataccgga gcctggaagt gcagcggcag cagaagatcg
     2341 ctctggacaa ttttccgaat ccctcgaaga agatggtcgt cacgccgtcg gacgagggcg
     2401 cgagcaatca ctgcgtcatg gaaaaccgcg ccctgatcgg ccaagtgctt ttcgactggc
     2461 tcgacgagac gatgggcgag tgaaagaggc gcaggtatcg ggctcgccat cgcggagacg
     2521 gctcacgaag attcatggtc ggaggctcct cttggagagc gaagagggcg gggggagccc
     2581 cgcttcacag cggaggttct tcaaggggag ggtgcgcccg ggaggcgtcc tccccttgca
     2641 tcttggattc ccggacatac ctaacgaggt ctccgggaat ccaggatgca acgtgatcga
     2701 tagggtcgac gcacagaaaa aatacggcct tttttgacga gaccacaacg aatccctcct
     2761 ttttcgcact ctctgctttc gtggcattat cgcccgcaag agcgcttcgc gtcaactcgc
     2821 aggcgtccag atgcgtaatg gcgaagcggt aagcagtcca cgaacgagag agatgatttt
     2881 cccgcttctc ttggaaaacg atccgcattc cgcgtgctac aatgacaggc gttgtagaaa
     2941 aatgcagttg tttttaagga aacaaaaatc ttctgcactc acaactttga agaggtgaat
     3001 tctatgaccg gaacccccgg cgcgaacaac aagaggaaac aaatcagctt tctgatcgcc
     3061 gacattaccg ccatgatctt gttctcgacg ggcctctgca tgatcatcga gatcttcgtc
     3121 gcgggactct cgttcttcca gtcggtcggg gcgagaatcg cggctattcc ggtcaacctg
     3181 atcaccggaa ggccgtacgg atggttcaga gacacgctct tcctgacgct cggcatcgac
     3241 aggtccagtc cggtgaaact ctttttcggc gacacgctgg cgttcgtcgt ctttcaggtg
     3301 ccgttgtacg tcctcgtcct gctctttgct ggggccacat ggaaacagat cgccgtcacc
     3361 tccctctccg cgtcgctggt cttctcgctg accggacggc cgtacggcat atttctggat
     3421 ttttgcaggc ggatcgccgt caaactcctc gggtgaaacg aacagcaagt aaaatcaagc
     3481 ggcgcgctcc ccgtctccct acaatacgtt gcgggtgggc ggaacggggt gaacgggcga
     3541 atgtacgagt ggcggcggca aattcaagcg atcgttgatg caatcgacga gtgtatcaaa
     3601 aaccatgatg acgaagcctt gacgctgcgc cgtctttcgt gcggattggg gtattccgaa
     3661 tttcatacca cgaggaaatt caaagagatt tcgggtatgc agttcaggga gtatctgcgg
     3721 gggagaagac tggccttcgc gctcaaagag gttcgggaca gcgggaagag tcttctggat
     3781 atcgcgttcg actacggctt ctcgtcgcac gaagcgttca ccagagcctt caaaggaacg
     3841 tacggcgtcg ctccgggcga ataccggaaa aaccccaagc ccgtcgtcct tcgcacgaaa
     3901 atcaaccctt tcgaccgcta ctttttagga ctgggagaga tcggcatgat gaaatccgca
     3961 gacgatgtaa aaatttactg tgtcaccatt cccgagcata agtttgtgca cataaaaaac
     4021 tacgagagca acggctattg ggacttttgg caaaaacaga gcggcattcc ggggcaggac
     4081 tgcgaaacga tttgcggcct gctcgacagc atcaagggca aattggacga cgacggcggc
     4141 agcgaatccg acagcggcag cgggcagatt atggcgtaca tcaacgaccc ggacggcaga
     4201 ctctgcgatt ggggtattcc gcgaacggag tgttacggcg cgcgcctacc tcttgactac
     4261 aaaggagaag tgccgccgca gatgcttctg atcgatattc ccgaggccga gtatatcgtc
     4321 ttcgaacacg ggccgttcga ttacgagcag gaaaaccgga gcgtggagga gaagatcgaa
     4381 cacgcgatgg cggcgttcga ttacgcggac gccggataca tccttgacac ctcccccgga
     4441 agaatcacgt acttttacca cgatccgggc cggtttttca agtacgtcag gccggtgcgg
     4501 aggaaataaa accgactgcc tctcacacca actcaatgac catcggcatg cattggaagt
     4561 gcgcgttcca tgcatgccaa aaggccctcg cgcgctcgcc cctcgtcgcc gcc
//
LOCUS       DAOPKU010000205         4613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20395_length_4613_cov_3.457437, whole genome shotgun sequence.
ACCESSION   DAOPKU010000205 DAOPKU010000000
VERSION     DAOPKU010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20395_length_4613_cov_3.457437"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PK849_07465"
     CDS             complement(<1..978)
                     /locus_tag="PK849_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66001.1"
                     /translation="MAQRKNRHTEAVGMERADELCTGKVCVLGEKRSVAAIMNALLEE
                     CSVDVPTVVLCHDPAIAERSARKEDAVVVMGDRPEAGERFPELLEPWLHSPEFGGNLD
                     SLVDALSAKLTDDLPVLTSNDAFWSAGALRLYNATLRACLLGMKWTRSFSLTDFARFH
                     EEIWKAFPAMVENDPGKNTYDYPRHYAWVKAFKALEEEIPFTGLLADVHVSTAQITMN
                     TCYSLTGGFRDLLKKIPMEHEGGESFLSFCDYLKDHRGGRLFLVLPRRMKSVAPALFR
                     FLLAGLEIAHLNCGEPDARLIIPDVSAWREPASLNALLREGPESFSVIWG"
     gene            complement(1069..1479)
                     /locus_tag="PK849_07470"
     CDS             complement(1069..1479)
                     /locus_tag="PK849_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66002.1"
                     /translation="MNKENTRTLFEAFPELYRGRFLDMKENLMCFGFECDDGWFELLY
                     DLSREITEHCAASGDPVPLAVQVKEKFGTLRFYADNTSEAVDDIIEKYQEKSGGICEV
                     TGAPGKLCHRRGRLKTLSEEEVRKDGFCPGTKTT"
     gene            complement(1555..3759)
                     /locus_tag="PK849_07475"
     CDS             complement(1555..3759)
                     /locus_tag="PK849_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66003.1"
                     /translation="MIGGILGGTASMRLGSALRNAEMESIKGRRLLVVGVQPRLFPVA
                     GVLPEALAPGFAHNRPVDVLAESYASVQGVDAAAAFFSEAALSGQGGGVHRDSNDVYW
                     FQSMRSGLASLFRIAGLLACRKGETRSLNRILCDCFDDLYDAVSRTSPNGTPERPKWL
                     EIIPEEVRRPVQLNYLGKAGPSTYSHLSTLAVAVGELRRGAPESKTAPFAAMNDGAPL
                     FIYAPAWGNAALNILLRICMRGNYALLLPEVHLWPEAELLRIGHALRYGASGCNVVWT
                     SLSVPAHPAFDSDWEIFGKSSSSDVLGLFRARLNRYGAEGESRLKDLKYETPASLDDL
                     HALQRTPSGWSIVPCTPFDERETVELEGEITSLSKEDIFLANMAELAVFAPEPPAAIG
                     PEEPFEEEFSPKAPRGSEKPLDSRTAGGSAGDKHKSGPLSGNAPEAKVHIVEGVPETI
                     GSGKYGYCTINGETILLKDVCIARIKYVAVLGKEVGKPQCPLAFYEKLWSAVIPPEKE
                     ILGPRVDAFVPVVVDPEKASPAGDGSMEVYSPRYGRSIRLVFHGLREMPSEKEFTAFG
                     TWKEPRSVLLVRSLLKPLAPLPPVSGKRRRATRLLTGRWKEVYSSGERLRDFMETALD
                     ELSDPSGLSDETSEKLASLRSEYEENASLAMDRLHEDGKPEKRLENRGVVCLDMETLN
                     NRDEAWEALCAADISSLPPAGYRERLKRRGLHEKYTGSLDAVFASLLLNLEL"
     gene            complement(3759..4355)
                     /locus_tag="PK849_07480"
     CDS             complement(3759..4355)
                     /locus_tag="PK849_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66004.1"
                     /translation="MQEKYTDMDDVRREDLQYILDILETMDEKESSGETWGSLAAAMK
                     EATEKKDPFFDRIESLLYSALLVYSFDKDADLLRSSMQKIASYAAALPAERIRPWLRR
                     SSQRIDLLLSFTDVWQPPCWEKMLGCPEPGLDEEWMDEMFGIAPGDCEYHSVPYLAFL
                     LAGTGFPFTGEELSGIRSGAGMSVGELRGNSRGKEGGA"
BASE COUNT          914 a         1381 c         1295 g         1023 t
ORIGIN      
        1 tccccagatg acggagaaac tctccggtcc ctcacggaga agcgcgttca gagatgcggg
       61 ttcccgccat gcggagacgt cgggtatgat cagtcgtgcg tcgggttctc cgcagttcag
      121 gtgcgcgatt tccaatccgg cgagaaggaa gcggaagagc gccggagcga ctgatttcat
      181 ccgtcgcggc agcacgagaa agagccttcc tccgcgatgg tctttcaggt agtcgcagaa
      241 cgagaggaaa ctctctcccc cctcgtgctc catggggatt tttttgagca ggtccctgaa
      301 gccgcccgtc agtgaatagc aggtattcat ggtgatctga gcggtgctta cgtgaacatc
      361 cgccagcaat ccggtgaacg gtatttcttc ttcgagggcc ttgaaggcct tcacccaggc
      421 gtaatggcgg ggatagtcat aggtgttttt ccccggatcg ttttccacca tcgccgggaa
      481 tgctttccat atctcttcgt ggaaacgggc gaagtcggtc aaactgaagc tcctggtcca
      541 tttcatcccg agaaggcagg cgcggagcgt cgcattgtac agtctgagcg ctccggccga
      601 ccagaatgcg tcgttgctgg tgagaacggg cagatcgtcg gtcagttttg cggagagagc
      661 gtcaacgaga gagtccaggt ttccgccgaa ctcggggctg tgaagccagg gttccagaag
      721 ctcggggaac cgctctccgg cctcgggcct gtcgcccatg acaacgacgg catcctcttt
      781 ccgggcggac cgctccgcta tggcgggatc atggcacaga acgacagtcg ggacatccac
      841 ggagcactct tcgagcagag cgttcatgat cgcggcgacg cttcgtttct ctcccagaac
      901 gcagactttt ccggtacaca gctcatccgc ccgttccatc ccgactgctt cagtatgccg
      961 attttttcgt tgcgccatag aacacatctc ccttgcggtc ttttagatag ttatatacta
     1021 tcttaagtat atctacttgt caagccgcgt ttgagacgct gaagaaatct acgtggtttt
     1081 cgttcccggg caaaagccgt ccttccggac ttcttcttcg gagagagtct tcagccgccc
     1141 cctcctgtga cagagcttcc ccggcgctcc cgttacctcg caaattccgc cggacttctc
     1201 ctgatacttc tcgataatgt cgtcaactgc ttcggacgtg ttgtcggcat aaaagcggag
     1261 cgttccgaat ttctctttca cctgtacggc caacggcacg ggatcgccgg atgccgcaca
     1321 gtgttcggta atttcacgcg aaaggtcgta cagaagttca aaccagccgt cgtcgcattc
     1381 gaagccgaag cacatcaagt tttccttcat atcgagaaac cttccacggt agagttccgg
     1441 aaaagcttcg aagagtgtgc gcgtattttc tttattcata gcggcgcctc ctgtttttca
     1501 tcataggcgg ttgcttcgcc cgtttccaac ggctttatcg ctattttcga tggtttagag
     1561 ctcaagattg agcagcagcg aggcaaaaac agcgtccaga ctcccggtgt acttttcatg
     1621 gagtccgcgc cttttcaggc gttccctgta gccggcggga ggaagtgagg agatgtccgc
     1681 cgcgcagagc gcttcccacg cttcatcccg gttgttcaat gtttccatgt ccagacagac
     1741 gactcctctg ttttcgaggc gtttctcagg ctttccatct tcgtgcaaac gatccatcgc
     1801 aagtgaggcg ttttcctcgt actccgaacg cagagaggcc agcttttcgc tggtttcatc
     1861 agacagcccg gagggatccg aaagttcgtc gagagccgtc tccatgaagt cgcgcagccg
     1921 ttctccgctt gagtacactt ccttccaccg tcccgtaagc agtcgggtag cccggcgcct
     1981 tttccctgag accggcggaa gcggcgcaag aggcttcagc aacgaacgga caagaagaac
     2041 gctccgcggt tctttccacg tgccgaaggc ggtgaactct ttttcggaag gcatctcccg
     2101 cagtccgtga aacacaaggc ggatggaccg cccgtagcgg ggagaataca cctccatgga
     2161 cccgtctcca gccggactcg ctttttccgg atccacgacg acgggaacga aggcgtcgac
     2221 tcttggaccc aagatctctt tttccggcgg gataacggcg gaccagagtt tttcgtaaaa
     2281 cgcgagcggg cactggggct ttccgacctc ctttcccaga acggcaacgt acttgatccg
     2341 ggcgatacaa acgtccttca gtaggatagt ctctccattt attgtgcagt agccgtattt
     2401 tccggaacct atcgtctccg gtactccttc gacgatgtgg accttcgcct ccggtgcatt
     2461 accggaaaga ggaccgctct tatgtttatc cccggcgctt cctcccgccg tcctggagtc
     2521 gagcggtttc tcgctccccc ggggcgcttt tggcgaaaac tcttcttcaa acggctcttc
     2581 cggtccgatt gccgcagggg gttccggagc aaatacagca agttcggcca tgttcgccag
     2641 aaaaatatct tcctttgaaa gcgaggttat ttccccctcc agttcgactg tttcccgttc
     2701 gtcgaagggg gtgcacggaa cgatgctcca tccggaggga gtgcgctgaa gcgcgtgaag
     2761 gtcgtccaac gacgccggcg tttcgtattt cagatccttg aggcgactct ccccttccgc
     2821 tccgtatctg ttgagccgcg cccggaagag ccccagcacg tcggaggacg aactcttccc
     2881 gaagatctcc cagtcgctgt cgaaggcggg gtgggcaggt acggagagag acgtccagac
     2941 gacgttgcat ccgctcgccc cgtacctgag ggcgtgcccg atgcgaagca gctccgcctc
     3001 gggccagagg tgcacctcag ggagaagaag tgcatagtta ccccgcatgc agatacgaag
     3061 caggatgttg agcgcagcgt ttccccaggc gggagcgtag ataaagagcg gcgccccgtc
     3121 gttcattgcg gcaaacggcg ctgttttact ttccggggcc cctctgcgga gttcccccac
     3181 cgctacggcg agcgtcgaaa ggtgcgaata ggtggaaggg ccggcctttc ccaggtagtt
     3241 cagctgaacc ggcctgcgca cttcttccgg tatgatttcc agccacttag gacgttcggg
     3301 agttccgttg ggcgacgtcc ggctgaccgc gtcgtacagg tcgtcgaagc agtcgcacag
     3361 gattctgttg agcgaacggg tttctccttt tctgcacgcg agcagccccg cgatccggaa
     3421 gaggctcgcc agcccggagc gcatgctctg gaaccagtag acgtcgttgg aatcgcggtg
     3481 cactcctccc ccttggccgg agagagcggc ttcggagaag aatgcggccg cagcgtctac
     3541 accctgcacg gaagcgtagc tctcggcaag gacgtcgacg gggcggttgt gcgcaaaccc
     3601 cggcgcgagc gcctcgggca gaacccccgc cacagggaag agcctgggct gcacgccgac
     3661 gacgagcagc ctccgtccct tgatggactc catctccgcg ttgcgcagcg ccgaaccgag
     3721 gcgcatggag gcagtacctc ccaggattcc gccgatcatt aggctccgcc ctcctttccg
     3781 cgcgaattcc cccgcagctc cccgacgctc attccggcgc cgctccggat accggaaagt
     3841 tcctccccgg tgaagggaaa accggttcct gcgagcagaa aagcgagata cgggaccgaa
     3901 tggtattcgc agtctccggg ggcaatcccg aacatttcgt ccatccactc ttcgtccaaa
     3961 cccggttccg ggcagccgag cattttttcc cagcagggag gctgccatac gtcggtgaag
     4021 gagagcagca agtcgatccg ctgagaagac ctgcggagcc acggccggat tctttcggcg
     4081 ggaagggcgg cggcgtagga agcaattttt tgcatcgagc tccggagaag atcagcgtct
     4141 ttatcgaagc tgtagacaag caacgcggag tacagaagcg actcgatacg gtcgaaaaac
     4201 gggtcttttt tttccgttgc ctccttcatt gcggccgcta aactacccca cgtctcgccc
     4261 gaagattcct tctcatccat tgtttccagt atatccagga tgtactggag gtcttcccgc
     4321 ctcacatcgt ccatgtccgt gtatttctcc tgcatgacgc agcctccctt cgtcttctca
     4381 acgatcattc tcttttaaga ccatcctaca ggtccaccag gtcagaataa ggggtagtaa
     4441 aaaacttttt cgtagaactt caaagaaacc ggccgattga ggctacgccg ctcccctctc
     4501 ttcaagctcc tgcggggggc acgcgagaga gcggatgacg actgagccat cgtgcacttc
     4561 gattacaagc ttcgtttttc cggaaggagc tccggcgata tgcccggcga gag
//
LOCUS       DAOPKU010000206         4583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20580_length_4583_cov_5.174028, whole genome shotgun sequence.
ACCESSION   DAOPKU010000206 DAOPKU010000000
VERSION     DAOPKU010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20580_length_4583_cov_5.174028"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2140)
                     /gene="uvrA"
                     /locus_tag="PK849_07485"
     CDS             complement(<1..2140)
                     /gene="uvrA"
                     /locus_tag="PK849_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPE66005.1"
                     /translation="MSQWIHIKGAREHNLKNVDVDLPREQLIVITGPSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGVQKKPDVDDISGLSPAISIEQKGTSHNPRSIVGTV
                     TEVYDYLRLLFGRVGTPYCPSCGKPVLRHTLDEIINYLLQDFSEKRLEILAPLVRGKK
                     GEFRNLFAQTREKGFLRVRVDGAVLWLEEEISLDKNKRHSIEAVIDRLRIREDRRSRI
                     AEAIETAFSLSDGYVLLLDEEGNERVLTEKFACADCELTFPEIEPRLFSFNTPYGACP
                     DCSGLGSHQYFAEELAVDPGRTITDGAILPWKKKHYMLTKLEKFAKHNDWDLSSPYGE
                     LPEHVRSFILYGTDERIPLTFRENGEEKSYMGRYEGLLNWLESRWKETESEGVIEELE
                     TYRADDLCQSCKGLRLRPEALYVRLGGYGIGDLISMPVDSLRQTLETISFTSSEEHVV
                     HQVLLEIKKRLDFLSDVGVGYLSLLRRADTLSGGESQRIRLATQIGSKLSGVLYVLDE
                     PTIGLHSRDTEKLVRTLEAIRDLGNTVVVVEHDRETMTAADSIIEMGPGAGEGGGSIV
                     FSGSYEDALHSTHLTGPYLRGERTAVIGEGRRTPKGWLTVKGASHNNLQGIDVSFPTG
                     VFVSVTGVSGSGKSSMVHDVLYKGLRRLLDRDFRERAGNHLTIEGSEQFRNVILVDQS
                     PIGRTPRSNPATYTGLFTLIRELFSGLPESR"
     gene            complement(2079..4112)
                     /gene="uvrB"
                     /locus_tag="PK849_07490"
     CDS             complement(2079..4112)
                     /gene="uvrB"
                     /locus_tag="PK849_07490"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPE66006.1"
                     /translation="MFIEKKFELHSDWAPSGDQPQAIETLAAGVAQGLRFQTLMGVTG
                     SGKTFTVANVIASAQRPVLVLAHNKTLAAQLYSEFKAFFPRNAVHYFVSYYDYYQPEA
                     YVPSTDTYIEKDASVNERIERLRLAATKALIERRDVIVVASVSCIYGLGLKEAYESVI
                     FPFAVGEQWERRAFLEKLLENYYDRNDIVLEPGKFRARGDIVEIFPAYGERALRIAFF
                     DEEIEQIDEFDPVSGKVIENLEKASIFPAQHYVTDRDAISNAVEPIQTEMELQAAEFE
                     KQGKFLEAQRIRMRTLYDLEMLRETGYCSGIENYSRYLDGRNPGEPPGTMIDFFPEDF
                     LLVVDESHITLPQVRGMFNGDRARKLTLVENGFRLPSCLDNRPLEWKEFEQHINQAIF
                     VTATPGDYEMQVSGKMVEQVIRPTGVVDPEVEILPAKGQVDDLISRLRDIVRNGQRAL
                     VTTLTKKSSEDLAEYLAELRLKVKYIHSELNAFERAELIRDLRTGDISVLVGINLLRE
                     GMDLPEVALVAILDADREGFLRSYRSLIQMMGRAARNICGKVILYADEITESIRKSVD
                     ETVRRRRLQMKYNEENNITPITIQKEIVHLLPEELLEDQPTGKGRGGAETFKELSVPD
                     MEKMMWQAVERLDFEKAARIRDALASLEGNEWNRVAMDTHKRSTRTQSKKRRR"
     gene            complement(4211..>4583)
                     /locus_tag="PK849_07495"
     CDS             complement(4211..>4583)
                     /locus_tag="PK849_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HPE66007.1"
                     /translation="VKLGHKHNEVFLGRDIGEERNYSDAIAYAIDQEVKAIIDSCYEK
                     VRNILVENSEEMEKIAKTLLEKEIIEGKELNALLGLENPEEEKVVGPEEEERTETQET
                     IAEENPVGESKNSDAATNAAI"
BASE COUNT         1006 a         1221 c         1154 g         1202 t
ORIGIN      
        1 ggcgcgattc gggaagcccc gaaaagagct cccgtatgag tgtgaaaagc cccgtatacg
       61 ttgcagggtt agaacgtggg gtccgcccta taggactttg gtcgacaagg atgacgttcc
      121 tgaactgttc gctcccttcg atcgtcagat gattgccggc cctttcccgg aagtccctgt
      181 ccaaaagcct tctgagacct ttgtagagta cgtcatgtac catgctgctc tttcccgagc
      241 cggagactcc ggtgactgag acaaagacac ctgtcgggaa cgaaacgtcg attccctgta
      301 gattgttgtg tgatgcaccc tttactgtca gccagccttt gggagtccgt ctgccctccc
      361 ctatcacagc ggttcgctcg ccccgaaggt aagggcctgt cagatgtgtt gaatgcaagg
      421 cgtcttcata gcttccggag aagacgatac tgccgccccc ttctccggcg ccgggcccca
      481 tttcaatgat cgaatcggcc gccgtcatcg tctctctgtc gtgttcaacg acgacgacag
      541 tattgccaag atctcggatg gcttcaagtg ttctgacgag tttttccgtg tcccgtgagt
      601 gaagaccaat tgtcggttcg tcaagaacgt agagaacgcc gctcaacttc gagcctatct
      661 gagtggcaag gcggattctc tggctctctc ccccgctgag ggtatcggcg cggcgtagca
      721 gagaaagata gcccactcca acatcggaga ggaagtcgag gcgttttttt atttccagca
      781 atacttggtg tacgacatgt tcctcggaag acgtgaagga gattgtttcc agcgtttggc
      841 ggagagagtc gacaggcatt gatataaggt cgccgatacc gtatcctcca aggcggacgt
      901 aaagagcctc ggggcgcagc ctgagccctt tgcaggattg gcaaagatca tctgcgcggt
      961 atgtctccag ctcttcgatt acgccttcgg attctgtttc cttccagcgg gattcaagcc
     1021 agttcagcag cccttcgtaa cggcccatgt agcttttttc ttcgccattc tctctgaacg
     1081 tcaaagggat acgctcatcg gttccgtaga gaataaacga ccgaacgtgt tcgggcaatt
     1141 ccccgtaggg cgacgaaaga tcccagtcgt tatgtttcgc aaacttctcc agttttgtga
     1201 gcatatagtg ttttttcttc catggcaaaa tggctccgtc ggtgatagtc cttcctggat
     1261 caactgccag ttcctcggca aaatactgat ggctccccag gccggaacag tcggggcagg
     1321 ctccgtaggg cgtattgaaa gagaaaagtc tcggttctat ctccggaaag gtaagttcgc
     1381 agtcagcgca ggcaaatttc tccgtgagaa ctctttcgtt cccctcttcg tcgaggagaa
     1441 gaacgtatcc gtcgcttaag gagaatgcag tttcaatggc ttcagctatc cgtgaacggc
     1501 ggtcctcacg tatacgaaga cggtcgatga cggcctctat agagtgtcgc ttgttcttat
     1561 cgagcgaaat ttcttcttca agccagagca cagcgccgtc gacacgaaca cgaagaaatc
     1621 ccttctcccg cgtctgggcg aagaggttgc gaaactcgcc ttttttccct cgaacaagcg
     1681 gggcaaggat ttccaaacgt ttctcggaga agtcctgcag gagatagtta ataatctcgt
     1741 caagagtatg acgaagaaca ggcttcccgc acgaagggca atacggcgtc cctactctgc
     1801 cgaagagaag ccggagatag tcgtacactt ccgtaaccgt tcctacgatc gaacgaggat
     1861 tgtgcgacgt ccctttctgt tctattgata tggcgggaga aagtcccgaa atgtcatcaa
     1921 cgtcgggctt tttttgtact ccaagaaact gtctcgcgta tgctgaaaga gactcgacat
     1981 atcttcgttg cccttctgca tagagcgtat cgaaggcaag cgacgatttt ccggaaccgg
     2041 acggtcccgt tatgacgatc agctgctcgc ggggcagatc aacgtctacg ttttttagat
     2101 tgtgttctcg tgctcctttt atgtgtatcc attgcgacac ggttccattc attcccttca
     2161 agcgaagcaa gggcgtccct gatgcgggcc gccttctcaa aatcaagccg ttcaacggcc
     2221 tgccacatca tcttttccat gtccggtacc gagagttctt tgaacgtttc tgctccgcca
     2281 cggcctttcc ccgtgggctg atcctcaagg agctcttccg ggagaagatg tacaatctct
     2341 ttttgtattg tgataggtgt aatattgttt tcttcattat acttcatctg cagtctgcgc
     2401 cttcgaactg tttcatccac cgatttcctg atactctcag tgatctcgtc agcatagaga
     2461 ataaccttgc cgcagatgtt tcgagccgcc cgccccatca tctgaatgag cgagcgatag
     2521 gatctgagaa agccttccct gtctgcgtcg agaatggcaa caagcgcaac ctcgggaagg
     2581 tccatacctt ccctcaggag attaatgcca accagaacgg agatatcgcc tgtgcggaga
     2641 tctcgaatga gttccgcgcg ctcgaaggca ttcagttccg aatgtatgta ctttaccttg
     2701 aggcgaagct ccgcaaggta ttccgcgagg tcttctgagg acttcttcgt gagcgtggtg
     2761 acaagagccc gttgtccgtt gcggacgata tcgcgcagac gggatatgag gtcgtcaacc
     2821 tgccccttgg cgggaagaat ttctacttcg ggatcaacga cgcccgtggg gcgtataacc
     2881 tgctccacca tctttccgga aacctgcatt tcataatcgc cgggagtagc cgtaacgaag
     2941 atcgcctggt tgatatgttg ctcaaactcc ttccactcca aaggccggtt atccagacac
     3001 gatggaagac gaaacccgtt ctcaacgagc gtgagctttc ttgccctgtc gccgttgaac
     3061 atcccccgta cctgaggcag cgttatatgc gattcgtcga cgaccaataa aaagtcctcg
     3121 gggaagaaat cgatcatcgt ccccgggggt tcgcccggat tccttccgtc aagataacgg
     3181 gagtagtttt ctatcccgga gcagtacccg gtttcacgga gcatttcaag atcatagaga
     3241 gtgcgcatac gaatccgctg cgcctcaaga aacttgccct gtttttcaaa ctcagccgcc
     3301 tgaagctcca tctccgtttg aatcggctcc acagcattcg agatggcgtc cctgtcggtt
     3361 acgtaatgct gcgcagggaa tatggaggcc ttttccagat tttcaatcac ctttccggag
     3421 acggggtcga attcgtcgat ttgttcaatc tcttcatcaa aaaaagcgat tcgcagggct
     3481 ctctctccgt atgcgggaaa aatttcgaca atatcacccc tggctcggaa tttgccgggt
     3541 tcgaggacaa tatcattcct gtcgtagtag ttctccagca gcttttcaag gaaagcgcgg
     3601 cgctcccact gttcacccac tgcaaaagga aagatgacgc tttcatacgc ttcttttaaa
     3661 ccgagaccgt atatacagga aacgctggcc actactatga catcgcggcg ttcaatgagc
     3721 gcctttgttg cagcaagtct cagacgctca attctttcat ttaccgaggc gtctttctcg
     3781 atgtacgtgt ccgtggaggg aacatacgcc tccggctggt agtagtcata gtagctgaca
     3841 aaataatgca ctgcgttccg gggaaagaac gccttgaatt cgctgtaaag ttgggcggca
     3901 agcgtcttgt tgtgtgcaag gacaagaaca ggacgttgtg cggaggctat tacatttgct
     3961 acggtgaacg tttttccgct tcctgtcaca cccataaggg tctggaatcg aaggccttgg
     4021 gcgacaccag ccgcgagcgt ttcaatcgct tgcggctgat ccccggaggg ggcccaatca
     4081 gagtggagtt caaacttctt ttcaatgaac atacggaatc cacccccaag tgaaagaaaa
     4141 gagggatccg gggagagcgt tgcgcccgtt cccggatcct tctgaagaca cggtattccc
     4201 agtttttccg ttatatagcc gcgttggttg cggcgtcgct gttcttactt tcgccgacag
     4261 gattttcttc ggcgatggtc tcttgcgtct cagtgcgttc ttcttcttct ggtcctacaa
     4321 ccttttcttc ttcaggattc tccagaccaa gaagagcgtt gagttccttc ccctcaatga
     4381 tctctttttc gagaagtgtt tttgctattt tttccatttc ttccgagttc tccacaagga
     4441 tgtttcggac tttctcataa caggagtcta tgattgcctt gacttcctgg tcgatagcgt
     4501 acgcgatcgc gtcgctgtag tttctttctt ctccgatatc tctgccaagg aatacttcat
     4561 tgtgtttatg tcccagtttt aca
//
LOCUS       DAOPKU010000207         4580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20603_length_4580_cov_4.196022, whole genome shotgun sequence.
ACCESSION   DAOPKU010000207 DAOPKU010000000
VERSION     DAOPKU010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20603_length_4580_cov_4.196022"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PK849_07500"
     CDS             <1..482
                     /locus_tag="PK849_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPE66008.1"
                     /translation="SISVFVAQNTGAGNKKRALKGFGEGLFALVALGVVASCILYLSA
                     EQSIALFIGTHQAAAIAEGVAYMRLVCFFYVLSYTGSSFVGFFRGSGRINIPFIGTTM
                     HLSVRVVLSYWLAPVMGLKAVALATGVGWVSIVLFQLVIFAAIRRRERKLELSGAPL"
     gene            674..1927
                     /locus_tag="PK849_07505"
     CDS             674..1927
                     /locus_tag="PK849_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66009.1"
                     /translation="MYIKRAVEDTVRKTAATFPVLLVTGPRQVGKSTLLERLAEPERR
                     IVTLDDPDVRYLAGNDPALFMQRFTPPVLIDEIQYAPELLPYIKMAVDRSGRSGDFWL
                     TGSQSFVMMKNVGETLAGRVGIIRLSGLSTSEIRGIPSKPFTTDPKSLLERAEEVQRL
                     ELNALYERIFKGSMPRLYADERVDWETYYRSYIDTYLKRDIKDLTQVADEMAFYNFMT
                     VVAARTSRPVVYEELAGECGISAPTAKKWLSVLVSSDIVAIVQPYFNNILKRVTKMPA
                     LHFLDTGLCAYLLKWGNPEALERGAMSGAFFESYVFSEVYKSYLNAGREPPLFYYRDK
                     EKREIDLLLYENGTLSPVEIKKSASPGKDAVKHFRVLEPLSDPENSGEPAGMKVGIGN
                     GAVVCMANDLLPVDSKNWYVPAWMI"
     gene            complement(2002..2670)
                     /locus_tag="PK849_07510"
     CDS             complement(2002..2670)
                     /locus_tag="PK849_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPE66010.1"
                     /translation="MFAASEEWKARYPDACFGVLIMEGVDNTGKCPALEERKDALEQS
                     LRTLYAGKSRKEIGAEHPFASYGRYYKTFGKNYHVLFQVESVALKEKPIFSPSPLVAV
                     MFMAELKNGLLTAGHDLDRTEPPFLLDVAKGGETFTSLGGTQRTLPPEDMFFSDRNGI
                     LSSIIYGPDDSSAITAATSRALFTVYGTPSISAGEVRAHLEEIEELVHILNPEATRKE
                     LLFL"
     gene            2798..4276
                     /gene="abc-f"
                     /locus_tag="PK849_07515"
     CDS             2798..4276
                     /gene="abc-f"
                     /locus_tag="PK849_07515"
                     /inference="COORDINATES: protein motif:HMM:NF000355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HPE66011.1"
                     /translation="MSLINVAHLTFAYEGGLENIFEDVCFRIDTDWKLGFTGRNGRGK
                     TTFLNLLLGRYEYEGTISSSVDFEYFPYDVPDEEMETLELIRSVAPGAEEWEIAREVS
                     LLDVADDALRRPFRTLSNGERTKVLLAALFLKENSFLLIDEPTNHLDMEARAAVTAYL
                     NRKKGFILVSHDRVFLDACIDHILSINRTNIEVQKGNFSSWWKNMQMREHYERSENER
                     LKKEIGRMQEAARRTSDWSDKVEKTKRGVRNSGLRPDTGYIGHKAAKMAQRSKSIDAR
                     RQSAIEEKSALLRNVEKSDELKLIPLVYHSPLLAELENVSIRYGERVACEGVSFKVGR
                     GERIALQGKNGSGKSSILKLLHRESLEYCGRLVVGSGVKISYVAQSADDLRGSPREYA
                     RVWGIDESLFRMVLDKLDFSKLQFEKDMADYSGGQKKKVLLARSLCEQAHLYIWDEPL
                     NYIDVISRIQITELLLEHKPTMLFVEHDRAFCDEVATKVVRL"
     gene            4340..>4580
                     /locus_tag="PK849_07520"
     CDS             4340..>4580
                     /locus_tag="PK849_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66012.1"
                     /translation="MTTTTEKLNELKARLRDVADLNSVGAVLHWDQATYMPPGGAVAR
                     GRQMAVVGRIAQELFSDLATGRLLDELRSYEESLPY"
BASE COUNT          945 a         1265 c         1417 g          953 t
ORIGIN      
        1 agtccatttc ggtgtttgtc gcccagaaca cgggggccgg caacaaaaaa cgcgcgctca
       61 aggggttcgg cgaggggctg ttcgcgctcg tcgcgctcgg agtcgtagca tcgtgtatcc
      121 tctacctctc cgcggagcag tcgatcgcgc tctttatcgg cacgcaccag gcggccgcga
      181 tcgcggaggg cgtcgcctac atgcggctgg tctgcttctt ttacgtcctc tcctatacgg
      241 gtagctcatt tgtgggcttt tttcgaggca gcggacggat caacatcccc ttcatcggga
      301 cgacgatgca tctgagcgtg cgcgtggttc tttcctactg gctcgctccg gtgatggggc
      361 tgaaggcggt ggcgctggca acaggcgtgg gctgggtctc catcgtgctg ttccagttgg
      421 tgatatttgc tgctatccgc cgcagggaac ggaaactcga actctccggc gcgccgctgt
      481 agtccgtggt tgcgtaaaaa ctctaagaac cgtcgcccca tcatgattgc gcttcccata
      541 gagttgtttg cctgctgtgg gagcggagtt ccggcaaaac gggcttgcgt cttcacagtg
      601 atttgactat aataaatatg tgttttagtt tggtcggata attggctccg ctcccggaaa
      661 cgaggtgagg tcagtgtata tcaaaagagc cgtcgaggat accgtcagga agacggcggc
      721 cacattcccg gtgctgcttg tgaccggtcc gcgccaggtc ggcaagtcga cgcttctcga
      781 acgccttgcg gagcctgaac gcaggatcgt gacgttggac gatcctgacg tcaggtatct
      841 tgccggaaac gaccctgcgc tttttatgca gcgattcacg cctccggtgc tgatcgacga
      901 aattcagtac gccccggagc tgctgcccta tatcaagatg gctgtggacc gttccggacg
      961 gagcggcgac ttctggctta ccggttccca atccttcgtg atgatgaaaa atgtcggcga
     1021 aactttggcc gggcgcgtcg ggatcatcag gctttcgggg ctttcgacca gcgagattcg
     1081 gggaattccc tcgaagccct tcacgacgga tccgaaaagc cttttggagc gcgccgaaga
     1141 agtacaaagg cttgagttga acgcgctgta cgagcgcata ttcaagggct ccatgccgag
     1201 gttgtatgcg gatgagagag tggattggga gacttattac cgttcctaca tcgacacgta
     1261 tctcaaacgc gatataaagg atctgacaca ggtggcggac gagatggcct tctataactt
     1321 catgaccgtt gtcgccgcca gaacatccag gccggtcgtc tacgaagaac tggcaggcga
     1381 gtgcggcatt tccgcgccga ccgcgaagaa atggctctcc gtgctcgtct catccgatat
     1441 cgtggcgatt gtgcagccgt acttcaacaa catattaaag cgagtgacca aaatgcccgc
     1501 gcttcacttt ctggatacgg ggctctgtgc gtatttgctg aagtggggta atcccgaagc
     1561 gctggagaga ggggccatgt ccggcgcttt tttcgagagc tacgtcttct ccgaagtgta
     1621 caagagctat ctgaacgccg gaagagagcc ccccctcttt tactaccgcg acaaggaaaa
     1681 acgggagatc gatctgttgc tgtatgaaaa cggaacgctt tctcccgtgg agatcaagaa
     1741 gtcggcatcc cccgggaaag acgctgtgaa gcacttccgg gtgctggagc ccctgagcga
     1801 ccctgaaaat tccggcgaac cggccggaat gaaagtcggg atagggaacg gggctgttgt
     1861 ctgcatggcc aacgacctgc tgccggtcga ttcgaaaaac tggtatgtcc cggcctggat
     1921 gatatgacga gggaactccg gcggtgaaag aagcgttctc tcctccgccg gagttccctt
     1981 tgcttcgtgc aaagcgatct atcagaggaa cagcagctct ttgcgtgtgg cttccgggtt
     2041 caggatatgc acaagttctt ctatctcctc caggtgcgcg cgcacctctc cggcggaaat
     2101 agacggcgtt ccgtacactg tgaagagcgc gcgggaggtc gccgcagtaa tggcgctgct
     2161 atcgtcgggg ccgtagatga tgcttgagag aatgccgttc cggtcggaga agaacatgtc
     2221 ctccggagga agagtacgtt gcgttccccc cagtgaagta aaggtttctc cccctttcgc
     2281 aacgtccagg agaaagggcg gctctgtccg gtcgaggtcg tgcccggcgg tgagcaggcc
     2341 gtttttcagt tcggccatga acatgaccgc cacaagcggc gacggcgaaa aaatcggctt
     2401 ttccttcaac gccaccgact cgacttggaa cagcacgtga tagttcttcc cgaaggtctt
     2461 gtagtatcgt ccgtaggatg caaaggggtg ttcagctcca atttcctttc tgctcttccc
     2521 cgcgtagaga gtgcggagac tttgctccag agcgtccttt cgctcctcca gcgcggggca
     2581 cttccccgta ttgtcaacgc cctccataat gaggacgccg aagcaggcgt ccggatagcg
     2641 cgccttccat tcttctgatg ctgcgaacat tgtgcaatcg tctcctcttt cgaagcgttt
     2701 cggtttatga tactaaaaat ccgggcggat gtcctgaagg gattcggaaa atcagaaagg
     2761 ccttcttgcg gttttttctt cacaggagga tatgctcatg tctttgatca acgtcgcgca
     2821 ccttaccttc gcctacgagg gcggattgga gaatattttc gaggacgtct gctttcggat
     2881 cgatacggac tggaagctcg gcttcaccgg caggaacggc agagggaaga ccacgttcct
     2941 gaacctgctg ctcggacggt acgagtacga ggggaccatc tcgtcgagcg tggacttcga
     3001 gtacttcccc tacgatgtgc cggacgagga gatggagacg ctggagctga tccggagcgt
     3061 cgcgcccggc gcggaggagt gggagatcgc ccgcgaagtc tcgctcctcg acgtggcgga
     3121 cgacgcgctc cgccgcccgt tccgcacgct ctccaacgga gagaggacca aggtgctgct
     3181 cgccgctctc tttctgaagg agaacagctt cctgctgatc gacgagccca ccaaccacct
     3241 ggacatggag gcgcgcgccg ccgtgaccgc ctacctgaac cggaagaaag gcttcatcct
     3301 cgtctcgcac gacagggtct ttctcgacgc gtgcatcgat cacatccttt cgatcaacag
     3361 gacgaacatc gaagtacaga aggggaactt ctcatcctgg tggaaaaaca tgcagatgcg
     3421 agagcactac gagcggtcgg agaacgagcg cctgaagaag gagatcggcc ggatgcagga
     3481 ggccgcccgc cggacgtccg actggtcgga caaggtcgag aagacgaagc ggggcgtgcg
     3541 gaactccgga ctgcgcccgg acaccgggta tatcggtcac aaggcggcga agatggcgca
     3601 gcggtcgaag tcgatcgacg cgaggcggca gtcggccatc gaagagaagt cggcgctgct
     3661 gcggaacgtg gagaagagcg acgagctgaa gctcatcccc ctcgtctacc actccccgct
     3721 tcttgcggag ctggagaacg tctccatccg ttacggagag cgggtcgcgt gcgaaggggt
     3781 gagtttcaaa gtcgggcgcg gggaacgcat cgcgcttcag gggaagaacg gcagcggcaa
     3841 gtcgagcatt ctcaagctcc ttcacagaga gtctctggag tactgtggaa ggctggtcgt
     3901 ggggagcggg gtgaagatct cgtacgtcgc ccagtccgcc gacgatctgc gggggagtcc
     3961 gagggagtac gcccgcgtct ggggaatcga cgagagcctg ttccgcatgg tgctggacaa
     4021 gctggacttc tcgaagctcc agtttgaaaa ggacatggcg gactacagcg gagggcagaa
     4081 gaagaaggtg ctgctcgcgc ggagcctctg cgagcaggcg cacctctata tctgggacga
     4141 accgctgaac tacatcgacg tgatctcccg catccagatc acggagctgc tgctggaaca
     4201 caagccgacg atgctcttcg tggagcacga cagggccttt tgcgacgagg ttgccacgaa
     4261 ggttgtgcgc ctgtaacgag cccttctcct gttcggggta taataagcgg gtgattttac
     4321 ctcgaacagg aggcttccca tgactacgac aacggagaaa ctgaatgaac taaaggcccg
     4381 tctgcgcgat gtggccgacc tgaacagcgt cggcgccgtg ctgcactggg atcaggcgac
     4441 ctacatgccg cccggcggag ctgttgcgcg cggacggcag atggccgtcg tcggccgtat
     4501 cgcgcaggag ctcttttccg atctcgcgac gggacggctg ctcgacgaac tgcgttccta
     4561 cgaagagagc ctgccgtatg
//
LOCUS       DAOPKU010000208         4578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20617_length_4578_cov_11.476233, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000208 DAOPKU010000000
VERSION     DAOPKU010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20617_length_4578_cov_11.476233"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1559)
                     /locus_tag="PK849_07525"
     CDS             complement(27..1559)
                     /locus_tag="PK849_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPE66013.1"
                     /translation="MYSREKRLKAVELYIKYDKSAADVIRELGYPDRHTLRSWYGMYL
                     KEQETGVIHDRYMRASKFTEEQKGTAVRHYLDHGRNYSRTVRLLGYPNRVTLRQWCLE
                     SAPEGCKKRKGRLHYTEEQKREAVEALCTRECSAREVADAHEVSRPVLYQWKNALLGK
                     EAGAVMTDAENGATQKTKEELLDELASLKKEIKQLKLERDVLRVAAEIIKKDPGVDLK
                     HLANREKTLLVDALRNEHPLKELLDCVKISRSSYFYQHGRLLAEDKYGEVKEKIGRLF
                     EENGGRYGYRRIHALLRREGERLSEKIVRRIMTENAFVVFGRKKRKYSSYQGEKTPGA
                     ENLISRDFHADAPNIKWLTDITEFPLPAGKVYLSPIVDCFDGMVVSWTIGTSPDAGLV
                     NSMLDMATATLGERERPVVHSDRGAHYRWPGWIERMEAAGLTRSMSKKGCSPDNAACE
                     GFFGRLKNEMFYNRKWQGVSIEEFMDILDNYLHWYNEKRIKISLGAKSPLEYRRSLDL
                     AA"
     gene            complement(1653..2396)
                     /gene="istB"
                     /locus_tag="PK849_07530"
     CDS             complement(1653..2396)
                     /gene="istB"
                     /locus_tag="PK849_07530"
                     /inference="COORDINATES: protein motif:HMM:NF038214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HPE66014.1"
                     /translation="MLKNEMKDELLACCKRLKLSRNMADNSELVEAEDREEYLLRLLK
                     LEVEHREKSRKDRLLKAAGFYSWKTFDGYRFDDVKLPSGVTPEYLARVGFVEEKKNLI
                     LYGNVGTGKTHLATAIGIEACRRGLSTGFYRTANLVNRLSEAKKTGRLGKLMTSLSKL
                     DLLICDEWGYVPLDREAAQLLFQIISDSYERRSVIITTNLEFSRWVGIFYDEQMTAAI
                     IDRLVHHSYLLLFDGPSNRVRDSLMRREG"
     gene            complement(2390..3919)
                     /gene="istA"
                     /locus_tag="PK849_07535"
     CDS             complement(2390..3919)
                     /gene="istA"
                     /locus_tag="PK849_07535"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HPE66015.1"
                     /translation="MLAMTRINDIRKAYFDEGCTISAIAAKFGIDRKTARKYIEQDDF
                     NKVPPVQSKRNRWPKLEPYMKRIDEWLEDDVKDRSKQRHTAKRVHARLEGTYPDFDCS
                     YRTVASYVKEKKREIYSDVRAALPLEHKAGEAQVDFGSADFYENGTLCSGKYLTVSFP
                     YSNAGYLQLFKGENRECLFEGLKAIFEHLGGVPTRLWFDNMSTVVKKVLKGGKRELCD
                     AFLRFKEHHGFDAAFCNANAGHEKGNVENKVGYHRRNLLVPIPEFERLDRYNEELLRL
                     CDEDHRREHYKRNSTIEALHREDKETLRPLPAVEFDCASYETMKTDLWGKFRISGVHT
                     YSTAPKHAGGRVLVRFTANSVVPLDENHRPITTHRRLYGSLKQEEMDWLPYLTQLSRN
                     PGAMKYSGIWEMLPDDLRGYLEGKEKSGQRDVLRTLALLSERDGFERAVKSVSEALRR
                     GIEDLDSLVALHGHLHGEYPADSMRIDETKLGSLPKLPEVRFAAKVYDGFLSAGGRLP
                     C"
BASE COUNT          832 a         1375 c         1152 g         1219 t
ORIGIN      
        1 gggggtgcgg atgttttttt ggactgctat gcggccaaat ccaagctcct tctgtattcc
       61 aacggactct tcgcacccaa agatatcttt attctctttt cattgtacca gtgcagataa
      121 ttatctaata tatccataaa ctcttcaatg cttactcctt gccatttcct gttgtaaaac
      181 atctcgttct ttaagcgtcc gaagaatcct tcgcaggcgg cgttatcggg agaacagcct
      241 tttttggaca tcgaacgcgt cagacctgct gcttccattc tctctatcca gcccggccat
      301 cggtaatgag ctcctctgtc ggaatgaact accggtcgtt ctctttctcc gagcgtcgcc
      361 gtggccatgt cgagcatgct gttcacgagt ccggcatcgg gacttgtccc tatggtccag
      421 ctcacgacca ttccgtcgaa gcagtcgacg atcggcgaga ggtagacttt gcctgcgggc
      481 agcggaaact ccgttatatc tgtaagccat ttgatgttcg gcgcgtccgc atgaaagtcg
      541 cgcgagatca ggttctccgc gccgggcgtt ttctctccct ggtacgagct gtatttccgc
      601 ttcttccttc cgaagaccac aaaggcgttt tcggtcatga tgcgacggac gatcttctcc
      661 gagagtcttt cgccttcccg acggagcaga gcatggattc tccggtatcc gtacctgccg
      721 ccgttctctt cgaacagtct cccgatcttc tccttgacct ctccgtactt atcctccgcc
      781 agaagacgcc cgtgctggta gaagtagctg ctgcgactta tttttacgca gtcgagcagt
      841 tctttcagcg gatgttcgtt tctcagggcg tcgaccaaaa gtgttttctc cctgttcgcc
      901 agatgcttaa gatcgacgcc cgggtctttt tttattattt ctgccgcgac tcgcaggaca
      961 tcccgctcga gcttcagctg cttgatctcc tttttaagac ttgcaagttc atccaggagc
     1021 tcttctttgg tcttttgcgt ggctccattc tcagcatccg tcatgacggc tccggcctcc
     1081 ttgccgagca gcgcgttctt ccactggtag agtaccggtc tggatacctc gtgcgcatct
     1141 gcaacctcgc gtgcgctgca ttccctcgtg cacagggctt ccacggcttc tcttttctgc
     1201 tcttcagtat agtgtagccg tcctttgcgt tttttgcacc cctccggagc cgattccaag
     1261 caccactgtc tgagggttac tctgttaggg tatcccagaa gtctgacggt ccttgaataa
     1321 ttgcgcccgt ggtcaaggta gtggcgaact gccgttcctt tctgctcctc ggtgaacttc
     1381 gaagctctca tatacctgtc gtggattacc ccggtttctt gttctttaag atacatcccg
     1441 taccatgaac gcaatgtatg gcgatctgga taaccgagtt cacggatcac atcggcggca
     1501 cttttatcgt attttatata aagctctact gctttcaatc ttttttccct tgagtacatg
     1561 gacatcctcc cgtctctgaa aaagagtcca agattttgtc cgcaccccca agttgcaaag
     1621 atttttcccc agcagggggg tggggaattt tgtcacccct ctctgcgcat cagggagtct
     1681 ctcacccggt tgctcgggcc gtcgaatagc agcaggtaac tgtggtgtac aaggcggtct
     1741 attatcgccg ccgtcatctg ctcgtcgtag aagattccaa cccatctgct gaattcaagg
     1801 ttcgtcgtga ttatcacgct ccttcgttcg tagctgtccg agattatctg gaagagcagc
     1861 tgcgcggcct cccggtccag ggggacatac ccccattcgt cgcagattag taggtcgagt
     1921 tttgagagcg acgtcatcag ctttcccagc ctgccagtct tcttcgcctc ggagagacgg
     1981 ttcaccaggt tggccgtgcg gtagaatccg gtgctcaacc cccttctgca cgcctctatc
     2041 cctatcgccg tggcaaggtg ggtctttccg gttcccacgt tgccgtaaag aatgaggttc
     2101 ttcttctcct cgacgaagcc gactctcgca aggtactcgg gcgtcacgcc cgacggaagc
     2161 ttcacgtcgt cgaaacggta gccgtcgaag gtcttccagc tgtagaatcc ggcagccttg
     2221 agaagacggt cctttctgct cttctccctg tgttccactt cgagcttcag gaggcgcagg
     2281 aggtactcct ccctgtcttc cgcctccacc agttcgctgt tgtccgccat gttccggctc
     2341 agtttgagcc gtttgcagca tgcgaggagt tcgtccttca tctcgttttt caacatggca
     2401 gtcttcctcc ggcggaaagg aatccgtcgt aaacctttgc cgcgaagcgc acctccggaa
     2461 gcttgggaag gctccccagc ttcgtctcgt cgatccgcat gctgtcggcc gggtattcgc
     2521 cgtgcaggtg tccgtggagg gctaccaggc tgtcaaggtc ctcgatcccc cgcctcaacg
     2581 cttccgatac gcttttcacg gcccgctcga atccgtcacg ctcgctcaac agggcgagcg
     2641 tccgcaatac atctctttgt ccgctcttct cctttccttc gaggtatccg cgaagatcgt
     2701 ccggcagcat ctcccatatc ccgctgtact tcatggctcc ggggttgcgg gagagctgcg
     2761 tcaggtaggg cagccaatcc atctcctcct gcttcagcga gccgtacagt ctgcggtgcg
     2821 tcgttatcgg acggtggttt tcgtccagcg gtaccacgct gttcgcggta aaccttacca
     2881 gcactcttcc tccggcgtgc ttgggagctg tggagtaggt gtggactccc gatatccgga
     2941 acttccccca caggtccgtc ttcatcgtct cgtagctcgc gcagtcgaac tcgacggcgg
     3001 ggagcgggcg cagcgtttcc ttgtcctccc tgtgaagcgc ctctatggtg ctgtttctct
     3061 tgtagtgctc cctgcggtgg tcctcgtcgc agagccgcag aagctcctcg ttgtaccggt
     3121 ccagtctctc gaactcgggg atcggcacga gaaggttgcg tctgtggtac ccgaccttgt
     3181 tctcaacgtt ccctttttca tgccccgcgt tcgcgttgca gaaggccgca tcgaagccgt
     3241 ggtgctcctt gaagcggagg aaggcgtcgc acagctcgcg cttccctccc ttgagcacct
     3301 tcttcacgac ggtcgacatg ttgtcgaacc acagccgcgt cggcacccct cccagatgct
     3361 cgaagatcgc cttcagaccc tcgaagaggc attcccggtt ctcacccttg aagagctgga
     3421 gatatcctgc gttgctgtag gggaaggaaa cggtgagata cttccccgag cagagcgtcc
     3481 cgttttcgta gaaatcggcg cttccgaaat ccacctgcgc ctctccagct ttgtgctcca
     3541 acgggagagc ggcgcggacg tcggagtaga tctcccgctt cttctccttc acgtaggagg
     3601 caacggtccg gtaagagcag tcgaaatccg gatatgtccc ctccagccgt gcgtgcaccc
     3661 tcttcgccgt gtgccgctgt ttgctccgat ccttgacgtc gtcttcgagc cactcgtcga
     3721 tccgcttcat gtagggctcc agcttcggcc aacgattcct tttggactga acgggaggaa
     3781 ctttgttgaa gtcgtcctgt tctatgtact ttctcgcggt cttccggtcg attccgaact
     3841 tcgccgcgat ggcgctgatg gtgcatccct cgtcgaaata cgcttttctg atatcattga
     3901 tgcgggtcat tgcgagcatc ctccattccc tccccttaaa gtgtctggat aacactcaaa
     3961 gggtaacggg gttcggtcct gctttcaatg gcccctttgc atttccgggg atttctccct
     4021 tgcacaactg gggaattcta ctttgcaact tctgccgttt ctattatgca ataaataacc
     4081 ggctccgcgc tataataggc catttctatt atgcaataaa ataccggttg ttagaatgat
     4141 cggcagatca aggaaacgga cgcagccgcg tatctcagcg gatggagcct tcaatcgccg
     4201 gaaaggttgg tttgaaaccg atgcgcttac tctcggatac gcctctcttc gacctgcaca
     4261 accatacttt ggccagtggc cattcgtacg gaacaatcgc gagtccgccg aagcagccgc
     4321 tgaacgggga ctcgccgtgc tcggtttctc cgaatatgcg tggacagtaa ccattttatt
     4381 acaataagta acttctccaa aaagagtccg agactttgcc cgcccccttt attggaaatg
     4441 ctccgagcgc tgtccaaaaa ctcgcgaagc ggcacggggc gttccgaacc gaaatacagt
     4501 ccgatccgaa tcacccttga tcgtgctggt cgttcagttc gaacgggcac agagtccatt
     4561 cgccggcctc gaaaaaga
//
LOCUS       DAOPKU010000209         4574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20640_length_4574_cov_5.537951, whole genome shotgun sequence.
ACCESSION   DAOPKU010000209 DAOPKU010000000
VERSION     DAOPKU010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20640_length_4574_cov_5.537951"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..1227)
                     /locus_tag="PK849_07540"
     CDS             complement(229..1227)
                     /locus_tag="PK849_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE66016.1"
                     /translation="MTKLLEVTNLEQRFDLNTDFLSKLVFEKGRIVKRERIVKAVNGV
                     SFSVGRREVFSIVGESGCGKSTVARTVVKLIEPKGGRICYDGRDITELSRTEMLPFRK
                     RMQMIFQDPYASLNPRQRVADILVEPMLFHKIARSPEEAREKALEILNRVGIRPEQAS
                     RYPHQFSGGQRQRIGIARALAVEPEFIVADEPVSALDVSIQAQILNLLMDLKEEFSFS
                     YLFIAHDLSVVKHISDNLAVMYLGRMVEKGSKAHIFGNPLHPYTLALFSAIPRISGTK
                     MRTGRNLEGEIPSAIDLPPGCCFHTRCPKALPVCREVVPQEREIEPGHFVSCHLFA"
     gene            complement(1224..2222)
                     /locus_tag="PK849_07545"
     CDS             complement(1224..2222)
                     /locus_tag="PK849_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66017.1"
                     /translation="MNDTLLEVKNLRTYFHTFKGTVKAVDDVSFSVGRGEILAIVGES
                     GGGKSVTGFSVIRLIEEPGVIESGEILFEGRDVMKLSEREVDRLRGKEISMIFQDPMT
                     SLNPVYTIGRQIEESLLLHTKLNREERRAACIDLLRSVGISNPESRLKAYPHQFSGGM
                     RQRVVIAIALAADPKLIIADEPTTALDVTIQAQILRLMADLVRAKNRSMILVTHDLAV
                     VSEMADRINVMYCGKIVETGSARKVIDESAHPYTKGLIDSIPDLERDRKRLNTIKGIV
                     PNMFELPEGCNFAPRCARATELCFGVEPEQREIGAGHFVACHFPLGGPAHEEEARS"
     gene            complement(2238..3137)
                     /locus_tag="PK849_07550"
     CDS             complement(2238..3137)
                     /locus_tag="PK849_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66018.1"
                     /translation="MTNTARSGRWRAFLDSEFFHNYRRSPSAVIGSILVLFVLLVAIF
                     GPLWTVQNPYNIASLELRDAYKPPAWIEDGEARFLLGTDQQGRDMLSAIVYGSRVSLI
                     IGVAGTLLASAIGIVLGLVAGFFGGRTDAIIMRIADIQLSFPTMLIALFLMSVLGRGV
                     TNILISLTLVGWVRFARTVRGETLSVKKREYVEAARVIGLPNIRILFKHVLPNVFASI
                     IVLATIQVGNFILTEATLSFLGLGVPITRPSLGMLCNNGFTVLYSGLWWVSILPGLYI
                     MIIVFGINLLGDFLRDELNPKLK"
     gene            complement(3154..4119)
                     /locus_tag="PK849_07555"
     CDS             complement(3154..4119)
                     /locus_tag="PK849_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66019.1"
                     /translation="MFRFIVKRLFQLVLVLFAVSLIVFLFTSVMGNPVYLMVKENAPA
                     ADIQAVTAALGLDKPLPVQYGIFIKNALQGNFGKSYMNHLPALGLIVERFPATLEIVV
                     VALLLSACIGIPCGVISGAYPKSRFSQVIMAFSIAGISMPSFWIGMVLIYYFSLHLGV
                     LPVSGRGAVGTIMGFKTSLATLDGWQHIILPSVTLALGTLATIIRLTRSGMQENMKQD
                     YVRFAKSKGVPRRTVLFGHALKNTLIPVVTIFGMQMGTLIAFTTITETIFAWPGMGKL
                     LIDSINSLDRPIIAAYILFVAVMFVFINFIVDILYVFIDPRIDLQ"
     gene            complement(4244..>4574)
                     /locus_tag="PK849_07560"
     CDS             complement(4244..>4574)
                     /locus_tag="PK849_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66020.1"
                     /translation="SDSGGESALIGLDMLYSITQNGEVKPGYGGVNRGFYCNPEVDKL
                     IDEAIATGDPAQRGEIMKKAWQLAADDVSYIPLYFEVDLYAHGKKITYTPRKDKYVFA
                     WDVSFND"
BASE COUNT          873 a         1375 c         1457 g          869 t
ORIGIN      
        1 gaaaggcgcc tccactgtaa atccgtgctt ttcgagcagc tccttttgcc atgcggcggc
       61 ccggacctcc tcgaaggagg tctcggggtt cgcgtggatg tcgtggctca ggcgcagcag
      121 ctccgacttc agttcgtccg ctcgtttcag aatacgttct ttcatttgta cacgtccttt
      181 atcgttggta tggcatcgag gccgggggaa gaaccccgcc tctcccgtct atgcaaaaag
      241 atggcacgaa acgaagtgcc ccggttcgat ctcccgctcc tgaggcacaa cctcccggca
      301 gaccgggagc gccttcgggc agcgcgtatg gaagcagcac cccggcggaa ggtcgatcgc
      361 gctggggatc tccccctcca ggttgcgccc ggtgcgcatc ttcgtccccg aaatgcgggg
      421 aatcgccgag aagagagcga gcgtgtaggg gtgcagcggg tttccgaaga tatgcgcctt
      481 gctccccttt tccaccatcc tcccgaggta catcaccgcg aggttgtcgc tgatgtgctt
      541 caccacggag aggtcgtgcg cgatgaagag gtacgagaag gagaactctt ccttcaggtc
      601 catcagcaga ttgagaatct gcgcctggat cgagacgtcg agcgcggaga ccggctcgtc
      661 ggcgacgatg aactccggtt ccacggcgag cgcgcgcgcg atgccgatcc gctgccgctg
      721 ccctccgctg aactggtgcg ggtagcgcga cgcctgctcg ggccggatgc cgaccctgtt
      781 cagaatttcg agcgctttct cccgggcctc ctccggcgac cgcgcaatct tgtggaagag
      841 catcggctcg acgaggatgt ccgccacccg ctgccttgga ttcagcgagg cgtaggggtc
      901 ctggaagatc atctgcatcc gtttccggaa ggggagcatc tccgtgcggg agagttccgt
      961 gatatccctg ccgtcgtagc aaatccgccc ccccttcggc tcgatgagct tcaccacggt
     1021 gcgggcgaca gtcgacttgc cgcacccgga ttcgcccacg atgctgaaga cctcgcgcct
     1081 gccgacggag aagctgacgc cgttgacggc cttcacgatc cgctcgcgct tgacgatacg
     1141 tcccttctcg aagacaagct tgctcaagaa gtcggtattc aggtcgaagc gctgttccag
     1201 gttcgtcact tcaagcagtt tcgtcatgag cgcgcctcct cttcatgcgc cggcccgccc
     1261 agcgggaagt ggcaggcgac gaaatgcccc gccccgattt ctctctgctc cggctcgacg
     1321 ccgaaacaca gctccgtagc gcgcgcacag cggggggcga aattgcaccc ctcgggcagc
     1381 tcgaacatgt tggggacgat gcccttgatg gtgttcagcc tcttcctgtc gcgctccagg
     1441 tcggggatgg agtcgatcag ccccttcgtg tacgggtgcg cgctctcgtc gatgaccttc
     1501 cgcgcggaac cggtctccac gatcttgccg cagtacatca cgttgatccg gtcggccatc
     1561 tccgagacga cggcgaggtc gtgggtgacc aggatcatcg agcggttctt cgccctgaca
     1621 aggtcggcca tcaggcgaag gatctgcgcc tgtatcgtca cgtcgagcgc cgtggtgggt
     1681 tcgtcggcga tgatcagctt ggggtccgcg gccagcgcga tggcgatgac cacccgctgc
     1741 cgcatgccgc cgctgaactg gtgcgggtag gccttgagcc tgctctcggg gttggagatg
     1801 ccgaccgacc gcagcaggtc gatgcaggcc gcgcgccgct cctccctgtt cagcttcgtg
     1861 tggagcagca gactttcctc tatctgccgt cctatggtgt acacggggtt cagcgacgtc
     1921 atcgggtcct ggaagatcat ggagatctcc ttcccccgga gacggtccac ctcgcgctcg
     1981 gagagcttca tcacgtcgcg gccctcgaag aggatctccc ccgactcgat gacgccgggc
     2041 tcttcgatca ggcggatgac ggaaaagccg gtgacggact ttccgcctcc cgattcgccg
     2101 acgatggcga ggatttcgcc ccgcccgacg gagaaggaga cgtcgtcgac cgccttgacg
     2161 gtccctttga acgtgtggaa gtacgtgcgc agattcttta cttcaagcag cgtgtcgttc
     2221 attcggtccg tccctcctca cttgagcttc gggttcagct cgtccctcag gaagtcgccc
     2281 agaaggttga tgccgaagac gatgatcatg atgtagagcc ccggcaggat ggagacccac
     2341 cagagtccgc tgtagagcac cgtgaagccg ttgttgcaga gcattcccag cgaggggcgg
     2401 gtgatgggga cgccaagtcc gaggaagctc agggtggcct ccgtgaggat gaagttgccc
     2461 acctggatcg tggcgaggac gatgatcgag gcgaagacgt tgggcagaac gtgcttgaag
     2521 agtatcctga tgttcggaag gccgatgaca cgcgcggcct cgacgtactc gcgcttcttg
     2581 accgagagcg tctcgccgcg caccgtcctt gcgaagcgga cccaccccac cagcgtcagc
     2641 gagatgagga tgttggtcac gccccttccg aggacggaca tcaggaagag cgcgatgagc
     2701 atggtcggga aggagagctg aatgtccgcg atccgcatga tgatcgcgtc ggtacggccg
     2761 ccgaagaaac cggccacaag cccgagcacg atgccgatcg cgcttgccag cagcgttccg
     2821 gccacgccga tgatgaggga gactctgctg ccgtagacga tggcgctgag catgtcgcgc
     2881 ccctgctggt cggttcccag gaggaaccgc gcctccccgt cctcgatcca cgcggggggc
     2941 ttgtaggcgt cccgaagttc gagggacgcg atgttgtacg gattctgcac cgtccacaga
     3001 gggccgaata tggcgaccag aaggacgaag aggacaagaa tggagccgat gacggcggag
     3061 ggagagcgcc tgtagttatg gaagaactcc gaatccagaa aggctctcca gcggccggag
     3121 cgcgcggtat tcgtcattat acgccgcccc ctttcactga agatcgatcc tgggatcgat
     3181 gaagacgtag agaatatcca cgatgaagtt gatgaagacg aacatcaccg ccacgaagag
     3241 gatgtacgcc gcgatgatcg gccggtcgag actgttgatc gagtcgatca gcagcttccc
     3301 catgccgggc caggcgaaga tggtctccgt gatggtggtg aaagcgatga gggttcccat
     3361 ctgcatgccg aagattgtga ccacggggat gagcgtgttc ttcagcgcgt gaccgaaaag
     3421 cacggtgcgg cgcggcacgc ccttggactt ggcgaagcgg acgtagtcct gcttcatgtt
     3481 ctcctgcatc ccggaccgcg tcaggcggat gatggtggcg agcgtcccca gcgccagcgt
     3541 gaccgacggg aggatgatat gctgccagcc gtcgagcgtg gcgaggctgg tcttgaaccc
     3601 catgatcgtt cctacggcgc ctcgtcccga aaccgggagg acgccaaggt gaaggctgaa
     3661 atagtagatc aacaccatgc cgatccagaa tgaaggcatg gagatgcccg cgatcgaaaa
     3721 ggccatgatg acctggctga agcggctctt cggataggcg cccgatatga cgccgcaggg
     3781 aatccctatg cacgccgaca gaagcagcgc gacgacgacg atttcaagag tggccgggaa
     3841 gcgttcgacg atcaggccga gggcgggaag gtggttcatg tacgacttgc cgaagttccc
     3901 ctgaagcgcg ttcttgataa atatgccgta ctgcacgggg agcggcttgt ccagtccgag
     3961 ggccgccgtg accgcctgga tatccgcggc gggagcgttc tccttgacca tgagatagac
     4021 cggattcccc atgacgctgg tgaagagaaa gacgatgagc gagactgcga agagcacgag
     4081 gacgagctga aacaatcttt tgacgatgaa acgaaacacg gttccacctc catacctatg
     4141 gtacagaata cggcggaccc tccgtcagag caaggtccgc cgctcttttc aaaaattccc
     4201 ggcgccggat agggcgcgcc gggaacctcg tcgcacattc gtcttagtcg ttgaaggaga
     4261 cgtcccacgc gaagacatac ttgtccttgc ggggcgtgta ggtgatcttc ttgccgtggg
     4321 cgtacaggtc cacctcgaag tagaggggga tgtaggagac gtcgtcggcg gccagctgcc
     4381 aggccttctt catgatttcg ccgcgctgcg ccggatcgcc ggtggcgatc gcctcgtcga
     4441 tcagcttgtc gacctcgggg ttgcagtaga agcccctgtt gacgccgccg taccccggtt
     4501 tgacctcgcc gttctgagta atgctgtaga gcatgtcgag cccgatgagc gcgctctcgc
     4561 cgcccgaatc ggac
//
LOCUS       DAOPKU010000210         4562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20696_length_4562_cov_4.182383, whole genome shotgun sequence.
ACCESSION   DAOPKU010000210 DAOPKU010000000
VERSION     DAOPKU010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20696_length_4562_cov_4.182383"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PK849_07565"
     CDS             complement(<1..679)
                     /locus_tag="PK849_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66021.1"
                     /translation="MSGSLKIKLTLLDNIIWLMLFLFFFACALGIPAFASWNNIVNIL
                     YHTTIMSMLVLAQGFVLLNGNFDLSIDAVLAFAPGVVILASTKWFPVALGNPYLAILL
                     TLLLGGCIGFFNGYCVAKLGMNAFMQTLSLSIILRGMVLFLIPLSIFPLDPVYSFIGK
                     ARFSAVGNLPAAIPVTFTIYFLFHILLRHTVFGRNYLATGGNARASFVAGIDTQKMVI
                     AGFVIAGL"
     gene            complement(664..2211)
                     /locus_tag="PK849_07570"
     CDS             complement(664..2211)
                     /locus_tag="PK849_07570"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPE66022.1"
                     /translation="MEEVCSKPVLLLEGISKVYPGTVALENIHFEALPGEVHGLIGKN
                     GAGKSTLVGILSGLVEPTSGTIMVNGRSFSSFSRSRAKREGISIVPQEPEIVLDLSVA
                     ENLFLGELENCSGFVSWSKIRADARRILDRFGLDIGADLQARDLSLSERQILLILKAC
                     VVEDANVVVLDEASASLSRNDAAILKRIVGELRESGKAVLYISHHIDELLDICDRLTV
                     LRDGRIVTTRTKNMLDHHSLAELIVGETLSSSGPERKEAGYIGEELLRLENLTRWGNF
                     CNISFSLKKGEILGIAGLRGSGRTELLKAISGIEPADAGKIFFKDKRVSFSTPSEALR
                     MGVAYLPEDRETEGLIQTFSIRENLLLNSISRFSRNGFVLRREAGRRAEEIFAGVQIH
                     AFSMEQSAEELSGGNKQKVVIGRIMANAPEVYLLDEPTRGVDIGAKRSILSIIHGPLR
                     AASGVLLTSPGLDDLMDICDRILVLYKGNMVREYQQKDFDEKSLFMDVQGFGYQDQRG
                     GDECRAV"
     gene            complement(2211..3470)
                     /gene="hisD"
                     /locus_tag="PK849_07575"
     CDS             complement(2211..3470)
                     /gene="hisD"
                     /locus_tag="PK849_07575"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPE66023.1"
                     /translation="MIVLKEAERNFQEEGGSVESVVGGILKSVRERGDEAVLAYTEKF
                     DGVRPSSLRIGKEEMRSAYEKVSPSTVKTLEFAAERIRNFALRQKSCMQELRYEISPG
                     VTLGHRLLPMESCGCYVPAGRYPLPSSALMSAIPAKVAGVRRIAACSPAGKGRDGIHP
                     AVLVAMDIAGVDEVYCMGGAQAIGAFAYGTGTIKGVDIIVGPGNRYVTEAKRQVSGAV
                     GIDMLAGPSEVVILADESANPEWVAADALARCEHDPHSWTVLVTTSRRLGESVMSVVE
                     TQAPLLSTGEFAFQSWRNNGRVLLAESLDEAVAITNDIAPEHLQVMTSDSPAISSQLV
                     NFASLFIGENAPVVFGDYASGPNHILPTVRCARFSNGVYVGTFLRVCSFQELTAEGAA
                     YLAGPCADFAEMEGLFGHKRSAEMRAR"
     gene            complement(3573..4340)
                     /locus_tag="PK849_07580"
     CDS             complement(3573..4340)
                     /locus_tag="PK849_07580"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPE66024.1"
                     /translation="MTFYDRFSVAGKVAVVTGAARGLGKGYALGLASAGATVLCGDVN
                     ENDAKLTAEEIRAFGGRAEGFFLDVTQPGKVEAVFSDAASRLGTLDIVVNNAGVEEIN
                     DFVSVTEEQYDKIMSVNLRGVFFTAQAAAKIMIKQRRGKIVNIGSLGSAIGLAQSSVY
                     CGAKGGVVQITRTMAIELAKYNIQVNAMGPGYFRTPMTEPFFQNPEHRRWIEERIPAG
                     RVGTAEDLLGTLVFLCSEASDYLTGQIVYIDGGWLAS"
BASE COUNT         1101 a         1292 c         1140 g         1029 t
ORIGIN      
        1 gaagcccggc tatgacaaaa cctgctatca ccattttttg tgtatctata cctgcgacga
       61 agctggcccg tgcatttccg cccgttgcca gatagtttct gccgaagacg gtatggcgta
      121 gcagaatgtg aaagaggaag tagattgtaa atgtcacagg tatagccgca gggaggtttc
      181 caacggctga aaaacgcgcc ttgccgataa aggaatagac cggatccaac ggaaatatcg
      241 aaaggggaat aagaaaaagg accattcccc gaagaataat tgaaagagaa agcgtctgca
      301 taaaggcgtt cattcccagc ttggcaacac agtagccgtt gaaaaaaccg atgcacccgc
      361 ccaggagcag cgtgaggaga atcgccaggt acggattacc caacgcgacg ggaaaccact
      421 tcgtcgaggc caaaataact actccgggcg caaaggcgag cacagcgtcg atagaaaggt
      481 caaaattgcc gtttaagaga acgaaacctt gtgcaagcac aagcatgctc attatcgtgg
      541 tatggtacag tatgttcaca atgttgttcc atgaggcgaa tgccggaatt cccagggcgc
      601 acgcgaaaaa aaagagaaag agcatcagcc agatgatatt gtccaaaaga gttaacttga
      661 tttttaaact gcccgacatt cgtcgccacc tctttgatct tgatatccaa aaccttgcac
      721 atccataaaa agagattttt catcgaaatc tttttgttga tattcgcgaa ccatgttccc
      781 cttgtacaga acaaggattc tgtcgcagat atccatcaga tcgtccagtc ccggcgacgt
      841 gagcagtacg ccggaggcgg cgcgcaacgg tccgtgaatt atggagagga tggaacgctt
      901 cgctccaata tccactcctc gcgtcggttc gtcgaggaga tagacctccg gtgcgttcgc
      961 cataatacgt ccaattacga ccttctgctt atttcctccg gaaagttcct cggcgctctg
     1021 ttccatggaa aacgcgtgaa tctgtacccc ggcgaagatc tcttcagcgc ggcggccggc
     1081 ttctcttctc aaaacgaagc cattccggga gaatctggaa atgctgttga gcagcaaatt
     1141 ttcgcggatg ctgaacgttt gtatcagccc ctccgtctcg cgatcctccg gaagatacgc
     1201 tactcccatg cgaagggctt ccgagggggt ggagaaagat actcttttgt ctttgaagaa
     1261 aatttttcct gcatcggcag gctctatccc cgagatcgcc ttcagcagct cggtccgtcc
     1321 gctcccccgc aatccggcga taccgagaat ttctcctttt ttgagggaaa agctgatatt
     1381 acaaaaattc ccccatcgtg taagattttc gagacgaagg agctcttcgc cgatatagcc
     1441 tgcttccttt cgttcggggc ccgaagaaga aagcgtctct ccaactatca gttcagcgag
     1501 cgaatgatgg tcaagcatat tcttcgtccg tgttgtgacg atacgtccgt cccgaagtac
     1561 ggtaagtcga tcgcaaatat cgagcagttc gtcgatatga tgggagatgt agaggactgc
     1621 cttccctgac tccctcaatt cacccacaat tcttttcaga attgcggcgt cgttccggct
     1681 gagcgacgca gatgcttcgt cgagcacaac aacatttgcg tcctcgacga cgcatgcttt
     1741 cagaataaga agtatctgcc gctcgctgag agagagatct ctcgcctgaa gatcagcgcc
     1801 gatatcgagt ccgaaacgat caagaatgcg ccgtgcatcc gcccggatct tgctccatga
     1861 aacaaaaccg gaacagtttt ccagctctcc caggaagaga ttctctgcaa ccgaaagatc
     1921 aagcactatc tccggttcct gtggaacaat ggagatcccc tcacgttttg ctcgcgatct
     1981 gctgaacgat gaaaaggatc gtccatttac catgatggtt ccggaggtcg gctccacaag
     2041 gcccgagaga ataccgacga gcgttgactt cccggctccg ttcttcccga tgagaccgtg
     2101 cacttctcct ggaagcgctt cgaaatgaat attctcaaga gccactgttc ccggatagac
     2161 cttgctgatc ccctcaagca gcaaaactgg tttggagcag acttcttcca tcaccgtgca
     2221 cgcatctccg ctgaacgctt gtgtccgaaa aggccttcca tttcggcgaa gtcggcgcat
     2281 ggtcccgcaa ggtaagcggc tccttccgca gtgagctcct ggaaggaaca gactctcagg
     2341 aaagtcccga cgtagacgcc gttcgaaaag cgggcgcacc tgacggtagg caaaatatga
     2401 ttcggtcccg aggcatagtc gccgaaaacg accggtgcgt tctccccgat gaagagggaa
     2461 gcgaagttta ccagttgtga cgaaattgcc gggctgtcgg acgtcatcac ttgtaggtgt
     2521 tcgggagcga tgtcgttcgt aatagccacc gcctcgtcca gagactctgc aagcagcacg
     2581 cgcccgttgt tcctccagct ttggaaagcg aattccccgg tggaaaggag cggcgcctga
     2641 gtttcgacga cgctcataac ggattcaccg agccgcctgc ttgtcgtcac aagtacggtc
     2701 cacgaatggg gatcgtgctc gcagcgcgca agagcgtcgg cggcaaccca ctccgggttc
     2761 gccgattcgt cggcgaggat caccacttcg cttgggcctg caagcatatc gatccctact
     2821 gccccggaaa cctgtcgttt tgcctcggta acatacctgt ttccaggccc gacgatgatg
     2881 tcaacccctt tgatcgtccc ggttccgtag gcgaaagcgc ctatcgcctg cgcgccaccc
     2941 atacagtaga cctcgtccac tcctgcgata tccattgcga caagaactgc cgggtgaatt
     3001 ccgtcccgtc ctttgcccgc cggagaacac gccgctatcc gccgaactcc ggcaaccttc
     3061 gccggaatag ccgacatcaa cgcggaagac ggaagagggt atctccccgc aggaacataa
     3121 caaccgcagg attccatggg gagaagccga tgcccgagcg tcactccggg agaaatctcg
     3181 taccgaagct cctgcataca gcttttctgg cgaagtgcaa agttgcggat tcgttccgcg
     3241 gcaaattcaa gcgtcttcac cgtcgaaggg gatactttct cataggcgga gcgcatctcc
     3301 tctttcccta tacgaagaga ggaaggacgg acgccgtcga acttctccgt gtaggcgaga
     3361 acagcctcgt ctccgcgttc gcggacactc ttgagaattc cgcctactac ggattcaaca
     3421 gagcctcctt cttcctgaaa attacgttct gcttctttga gtacgatcac ttcacattcc
     3481 tcctgatcgg tctggaaact cccgtctatt ggagcgggag caaaaagaga gagccgagaa
     3541 gatgttcatc tctcctgtac attacaattt cctcagcttg cgagccagcc gccgtctatg
     3601 tagactatct gtcccgtcaa ataatcggag gcttcactgc aaagaaagac cagcgttccc
     3661 agaagatcct cggcagtccc gactcgcccc gccggaatcc gctcctcgat ccaccggcgg
     3721 tgctcagggt tctgaaagaa cggctcggtc ataggagtac ggaagtatcc gggacccata
     3781 gcgttcacct ggatgttgta cttcgccagc tcgatagcca tcgtccgggt gatctgcacc
     3841 actccgccct tggcgccgca atagaccgag gattgcgcca gtccgatggc gctccccagc
     3901 gacccgatat tgacaatttt gccgcgccgc tgcttgatca tgattttagc ggctgcctgc
     3961 gccgtgaaga agacgcccct gagattgacc gacataatct tgtcgtactg ctcctccgta
     4021 acggagacga aatcgttgat ctcctcgacg cccgcgttgt ttacaacgat atcaagagtt
     4081 ccaagacggg aggctgcgtc ggaaaataca gcttccactt ttcccggctg cgtcacatcg
     4141 agaaaaaagc cctctgcccg gcctccgaag gctcgtatct cttcagccgt caacttcgca
     4201 tcgttttcgt tcacgtcgcc gcataataca gtcgcgccgg cagacgctaa tccaagcgca
     4261 taccccttcc caagtccccg ggctgctcca gtaacaacgg cgactttccc ggccacgctg
     4321 aatcgatcat aaaaagtcat ttgattcgct ccttgattgt gaatcgcggt gtctgtcctt
     4381 tcgctttcct gagagaggag aaaacagatg tcttgatgat gcttcatcat gttagcataa
     4441 actttcaaca atgcaagcgg tgaaaaccgt aagtctactt gtttgtgaag ctgctctgct
     4501 ggaatcgttc gtcgtgtata ataggaaaat caaagcgggc ttctgcctcg ccaaacatca
     4561 tc
//
LOCUS       DAOPKU010000211         4542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20811_length_4542_cov_4.702251, whole genome shotgun sequence.
ACCESSION   DAOPKU010000211 DAOPKU010000000
VERSION     DAOPKU010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20811_length_4542_cov_4.702251"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PK849_07585"
     CDS             <1..1063
                     /locus_tag="PK849_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPE66025.1"
                     /translation="ALAAQVLPQASTANPLDLVATAKPEHYAAAAKAMVESGLYDAFV
                     VVMVPPTGVETGPVAEAMIPVLKASGLPVTSCFFGPSMGEGGRAAMLNAGIPSFPFPE
                     QTASVLSLMREKTLSPCAEKPNTQTPSLLRKGALRKRLAASADGFLLPALCEELLEAY
                     GIPLPLSAMVTAPEEVKGASFLYPVVAKIDHSDVLHKSDAGGVVLNIADEKSLRGVVD
                     TLLARFPGARGVLVQEQIGKGIELILGADSDPLLGHAVLVGWGGTGVEVFSDVALGHV
                     PFSRAEGVRLLERLQCFPLLKGYRGKPGVDMEGLLDLMQKLQRLLLDFPEIEEMDLNP
                     LIWDSKRFIAADYRIKTRS"
     gene            1140..2372
                     /locus_tag="PK849_07590"
     CDS             1140..2372
                     /locus_tag="PK849_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HPE66026.1"
                     /translation="MGYREDGKWWVSPANYEAEVTKTFSFAPKIEILDTTLRDGEQQP
                     GIIFTKEDKVAIAKKLDEVGVHRIEAGTPAGSQEDADAIKAICDLGLKSKIFAFCRAM
                     PKDMELAKSLGVDGVLCEMIGSEQMLRYGKRWTPEQAIAACIEATQAAHELGMFVTFF
                     PADGSRAEMNYLLDFVQAVAEGGHIDSLALVDTFGTFSPEGAVYMLKKLKERFSFPIE
                     AHFHSDYDIGVATTLAALKEGASVAHVTVNGLGERAGSCPLEPLALSLEALYGQSTGI
                     VLNKLTELSKLVEELSRFPVPPIKPVVGNKLFGWETGLPSSLWTNAKTENPLIMLPYH
                     YSLTGREEPVLYIGKKSGKDNVKYWLAKTGLSLDDEGEKTLLQKVKDLSISLKRDLNE
                     DEFRGLVSRVKEESACNQ"
     gene            2360..3526
                     /locus_tag="PK849_07595"
     CDS             2360..3526
                     /locus_tag="PK849_07595"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPE66027.1"
                     /translation="MQPVKIAQRFQNIQPSGMLKIFQAAAAIDDLSNLGIGEPDFHTE
                     PKIVDAAAKAAKEGMTHYPPLTGYPELRKEICAYWKRHHDLEVTPEEIHITVGGIQAP
                     HLCFQAMLDPGDEVLIAEPCFTAYFQQIEFNGGVVVPVPSRAENGFFPTAGDFEKAIT
                     PRTKILVINSPCNPTGGVIMREQAQAIAEVAKKHDLFVISDEIYEAFVFSGKHIPMAS
                     LPGMKERTMTIGGFSKSHCMTGWRIGYVMAPAELLKVMVQLSVVQTFGVNVLSQMGGI
                     EALKTQDAKMRERTAEYEKRVRYSADRLNAMPGVSCPEPKGAFYLFPDISGTGMTDEE
                     FVWWLLETAKVATIPGTAFGESGAGHIRIACTLPMEGLKKAFDRMETALKNRKN"
     gene            3777..>4542
                     /locus_tag="PK849_07600"
     CDS             3777..>4542
                     /locus_tag="PK849_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPE66028.1"
                     /translation="MLTKRFLPAFFILCVLLVGCAFAASSEQPKTNLPFTVIAADVDE
                     VIIYEGDDEEVQPLYAVLMGVGEERAIKGLVSGDNVTTQRIRWSIDNWGVARIVGQEN
                     EGDVIEANEIAVEAWGNGTATIIATSVQDPTISEFCTVYVQNSPVPVEEVTIDKEEIG
                     ILSGYFGELTASVLPPQADFRHISWHSSNPEIVAIDPTMRTGNRTRLLTAGLHTGTAI
                     ISAVSNYSGGRGGERPYATCMVHHYVEGPRSTEGGCS"
BASE COUNT         1001 a         1310 c         1404 g          827 t
ORIGIN      
        1 ggcgcttgcc gcgcaggtac ttcctcaagc gtcgacggcg aatccgctcg accttgtggc
       61 cacggcaaaa ccggagcact acgcggccgc cgcgaaggca atggtcgaaa gcggcctgta
      121 cgacgcgttc gtggtggtga tggtaccgcc gacaggcgtc gagacgggcc ccgtggccga
      181 ggcgatgata cctgtcctca aggcgtccgg actgcctgtg acgagctgtt tcttcggccc
      241 ttcgatggga gagggcgggc gggcggcgat gctgaacgcg ggaatcccgt cgttcccgtt
      301 cccggaacag actgcttccg tactctcctt gatgcgcgag aagactcttt ccccgtgtgc
      361 ggaaaaaccg aatacgcaga cgccctcgct gctccgcaag ggagctctac ggaaacgact
      421 cgccgcttcc gcggacggtt tccttctgcc tgcgctctgc gaggagcttt tggaagcata
      481 cggcattcct ctccccctct ccgccatggt caccgcgccg gaagaggtca agggcgcttc
      541 gttcctctac cccgtcgtgg cgaagataga ccactccgac gtgctccaca agtcggacgc
      601 gggcggcgta gtgctgaaca tcgcggacga gaagtcgctc cgcggcgtcg ttgacacact
      661 cctcgcccgt tttccgggag ctcgtggggt actggtgcag gaacagatag gaaaagggat
      721 tgaactcatc ctcggcgcgg acagcgaccc gctccttggg cacgccgtgc tcgtcgggtg
      781 gggcgggaca ggtgtcgagg tcttctccga cgttgcgctg gggcacgttc ccttttcgcg
      841 ggccgagggg gtgcgccttc tcgaacgcct ccagtgcttc ccgctgctga agggctaccg
      901 gggaaagcct ggcgtcgaca tggaaggact gcttgatctg atgcagaaat tgcagcgcct
      961 gctgttggat tttcccgaga tcgaggagat ggacctcaat ccgcttatct gggacagcaa
     1021 acgttttatt gcggcggact acagaataaa aacacgctct tgacacgaag aaccggacgg
     1081 cggggacacc cacgccgccg gacgcaaaga agaacactac tttcgaggag gaattgagaa
     1141 tgggatacag agaagacggc aagtggtggg tttcacccgc gaattacgag gcggaagtga
     1201 cgaagacgtt ctctttcgca ccgaagatcg aaatacttga cacgacgctc cgcgacggcg
     1261 agcagcagcc ggggatcatc ttcacgaagg aggacaaggt cgccatcgcg aagaagcttg
     1321 acgaggtggg cgtccacagg atcgaggcgg gaacgcccgc gggatcccag gaggacgccg
     1381 acgcgatcaa ggccatctgc gaccttggat tgaagtcgaa gatcttcgcc ttctgccgtg
     1441 cgatgccgaa ggacatggag ttggcgaagt cgctcggcgt ggacggcgtg ctctgcgaga
     1501 tgatcggcag cgagcagatg ctgcgctacg gcaagcgctg gacgcccgag caggccatcg
     1561 ccgcgtgcat cgaggcgacg caggcagccc acgagctggg gatgttcgtc acattcttcc
     1621 ccgcagacgg ctcgcgcgcc gagatgaact acctgctcga cttcgtgcag gcggtcgccg
     1681 agggcgggca catcgactcg ctggcgctcg ttgacacctt cggcacattc tctcccgaag
     1741 gagcggtcta catgctcaag aaactcaaag agcgtttctc cttcccgatc gaggcgcatt
     1801 tccacagcga ctacgacatc ggcgtggcga cgacgctcgc cgcgctcaaa gagggcgcgt
     1861 cggtcgccca cgtgacggtg aacggtctcg gcgagcgcgc gggaagctgc ccgctcgagc
     1921 cgctggcgct ctcccttgag gcgctctacg gccagtccac cggaatcgtc ctcaacaagc
     1981 tcacggaact ctcgaaactg gtggaggagc tttcccgctt ccccgtgccg cccatcaaac
     2041 cggtcgtcgg caacaagctc ttcggctggg aaaccggact tccgtcgagc ctctggacaa
     2101 acgcgaagac ggaaaacccg ctcatcatgc tcccctacca ctactcgctg actggacggg
     2161 aggagccggt gctctacatc ggcaaaaaga gcggcaagga caacgtaaaa tactggcttg
     2221 caaaaaccgg actttcgttg gatgatgagg gagagaagac gctgctccag aaggtgaagg
     2281 atctctccat ctctctgaag cgcgacctca acgaagacga gttccggggg ctggtctccc
     2341 gggtgaagga ggagagtgca tgcaaccagt gaaaatagcg caacgctttc agaacatcca
     2401 gccgtcgggg atgctgaaga tcttccaggc ggccgcggcc atcgacgacc tgagcaacct
     2461 cgggatcggc gaaccggact ttcacaccga gccgaagata gtcgacgcgg cggcgaaggc
     2521 cgcgaaagag gggatgacgc actacccgcc cctcacaggg tatccggagc tgcgcaagga
     2581 gatatgcgcc tactggaagc ggcatcacga cctggaggtc acgcccgagg agattcacat
     2641 caccgtgggc ggcatccagg cgccgcacct ctgcttccag gcgatgctcg acccgggcga
     2701 cgaggtgctt attgccgagc cgtgcttcac ggcctacttc cagcagatcg agttcaacgg
     2761 aggagtggtc gtcccggtac catcgcgggc cgagaacggc ttcttcccga ccgccgggga
     2821 cttcgagaag gcgatcacgc cgaggacgaa gattcttgtg atcaactctc cgtgcaaccc
     2881 tacaggcggc gtgattatgc gtgaacaggc gcaggccatc gccgaggtgg cgaagaagca
     2941 cgatctcttt gtgatctccg acgagatcta cgaggccttc gtcttctcgg ggaaacacat
     3001 ccccatggcg tcgctcccgg gcatgaagga gcggacgatg accatcggcg gtttctcgaa
     3061 gagtcactgc atgaccggct ggcgcatcgg ctatgtgatg gcgcccgctg aattgctgaa
     3121 agtaatggta cagctctccg tggtccagac attcggcgtc aacgtcctct cccagatggg
     3181 agggatagaa gcgctgaaga cgcaggatgc gaagatgcgc gaacgtaccg ccgagtacga
     3241 gaagcgcgtc cgctacagcg ccgaccgcct gaatgcgatg ccgggcgtga gctgcccgga
     3301 gccgaagggc gctttctacc tcttccccga catctcgggg accggaatga ccgacgagga
     3361 gttcgtctgg tggctgcttg agaccgcgaa ggtagcaacg attcccggaa cagccttcgg
     3421 agaaagcggc gcggggcaca tccgtatcgc ctgtaccctc ccgatggagg ggctgaagaa
     3481 ggcgttcgac aggatggaaa ccgcgctgaa gaatcggaag aactaaagca agggaagaag
     3541 cgtacgcacg ggcgggggcc gtacatgctc cccgcctttt ttctgtgctg aaacaattcc
     3601 gccgggatga gaagcgtcca atcagaaaga acggattgcc aaggacgtga aagaaagtga
     3661 gaaaaaaatc cttcaatccg acgccaaatg ctcagaaaag tcgctcccct ttgagaacgc
     3721 cgtgctataa ttggtggtga tgtttttatt cgagcataag gacggtgtgg acaagtatgc
     3781 tgacaaaaag gtttcttcct gcgtttttta tcctctgcgt gctgttggtc ggctgtgcat
     3841 tcgcggcttc ctcggaacag ccaaagacaa acctcccttt cacagtaatt gccgccgatg
     3901 ttgacgaagt tatcatctac gagggagacg acgaagaagt acaacctctc tacgctgttc
     3961 tgatgggcgt cggggaagag cgggcgatca aggggcttgt atcgggagac aatgtaacga
     4021 cacagcgcat acgctggtct atcgataact ggggagtcgc aaggattgtc ggacaggaga
     4081 acgaaggcga cgtgatcgaa gcaaacgaga tcgcggtgga agcgtgggga aacggaactg
     4141 ccaccataat tgcaacttct gttcaggatc ccactatttc cgaattttgc accgtgtacg
     4201 tgcaaaattc gccagtcccg gtagaggaag tcaccatcga caaggaagag atcggtatcc
     4261 tcagcggcta cttcggagaa ctgacagcga gcgtcttgcc gcctcaagca gattttcgtc
     4321 atatttcctg gcattcgagc aatccggaaa ttgtagctat agaccctaca atgcgaacag
     4381 gtaaccgcac acgccttttg actgcaggac tccacacagg tacagcaatt atctccgccg
     4441 tctcgaatta cagcggagga cgcggcggcg aacgtcccta tgcaacatgc atggtccatc
     4501 actacgtgga aggcccccgt tcgaccgagg gcggttgcag cg
//
LOCUS       DAOPKU010000212         4536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20844_length_4536_cov_4.332961, whole genome shotgun sequence.
ACCESSION   DAOPKU010000212 DAOPKU010000000
VERSION     DAOPKU010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20844_length_4536_cov_4.332961"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PK849_07605"
     CDS             <1..610
                     /locus_tag="PK849_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPE66029.1"
                     /translation="KVTHHARADAKALLELRASINETLEKEERVSVTDMLVKITAMAL
                     RQKLFMNVSLRGDRIVVFREVHLGVAVALEGSLIVPVIRNADEKSLTRISRELKDLSR
                     RARENALAPEEISGGTFTLTNLGGYRSTEFFTPVINQPESAILGVGRTNDMPVAMDGE
                     VAVRPMTALSLAHDHRVIDGAPAAEFLALLIELIEHPFRVFL"
     gene            669..1178
                     /locus_tag="PK849_07610"
     CDS             669..1178
                     /locus_tag="PK849_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPE66030.1"
                     /translation="MESWKGCAYTALIVFGYLVRGNKVLIVRRANDPYKGQRTVPGGR
                     KQRDETLREALVREIEEETGCRVRSMEYAGMLHAIVEGRDMEYLSVYFISRDFDGEPR
                     DSEEGTVEWMDIDESLTMEDMHPIYLRLVPYILKGNYPMEGAMRISADDASEYMLFGP
                     GEHPAPEAR"
     gene            complement(1256..2164)
                     /locus_tag="PK849_07615"
     CDS             complement(1256..2164)
                     /locus_tag="PK849_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HPE66031.1"
                     /translation="MRLITRSDFDGLICGMLLKEAGIIDSWQFVHPKDLQDGLFQPTE
                     DDVLANVPYVPGCGMWFDHHTSENDRVGWSKDVQGESRLAPSAARIIYEYYGGAEKFP
                     AYGDMIEAVDKVDSGQLTREEILNPKGWILLGFLTDPRTGLGRFREFRISNYQLMEEL
                     INHCRNMRIGEILEQPDVKERVELYFEQTRRFTEMVHTHTKVEQNVIVTDLRDVSPIY
                     TGNRFLIYSLYPEQNVSVWIVDGRQKQNVSIACGYSILNRTCGVDIGALMLKHGGGGH
                     RMVGTCQVPYSEADDRIREIVDALKG"
     gene            2331..4247
                     /locus_tag="PK849_07620"
     CDS             2331..4247
                     /locus_tag="PK849_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE66032.1"
                     /translation="MVLFYNNDEEDASMTDMRRKTTACPYDCPDACAMKGMFDGASVT
                     LEPNRDLPYTTFLCGKGLRWASRATSSERLTTPMLRREGALVPVSWDEAVDVWAEKVR
                     SAVSRHGPLSLMHLSSAGSLYFSKLLLPHLFAALGGYTTKKGNLCSSAGSFGLAESFG
                     EVPVTRPESLSDHARGVLFWGRNALETHAHVVPLLQRVRGRGGEIASLEIRETPTTRF
                     ADRWWRVNPGGDWALAAWLCARLLEEGASDGWRERVENPEEFERTARSLDGAFLLRTA
                     GVSAECASELLEWLMRFAPVTHYAAFGAQRYLHGDAQFRWIGALAVLLGAFSGPGAGL
                     AFSKDEMALFPFALLPKVAYVRRLPVATWPVELESLDPPVEALSISGANPVRQSPGSD
                     FVREALRKIPFTVCIDFVLSDTAKECDLVLPTTTFLEEEGDWKGSYWHNYVVRSERIL
                     PPRGESLEETEIFTRLARRLGLPLDLTEMKREVDRAMLASPLLERVGEGVFRWDEPDY
                     WCASQRKAALPVSVPEAAEGREGALRLVTVHRKEYINGQSWDAPSVEAIPRLSVHPLD
                     AAFFDLKEGDEAVARGRRRGALRVVVREDASLGRGYCVLPQGTEGVNSLTEPLASPGF
                     GAPFAESWITLGKG"
     gene            4272..>4536
                     /locus_tag="PK849_07625"
     CDS             4272..>4536
                     /locus_tag="PK849_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66033.1"
                     /translation="MRMRFVLYLVAGYAVLVTIAFFFADKLLFFPHRELWATPSERGL
                     PWEDVRFEAADGTPLHGWYVPGAGRGPGGAAKATVLFCHGNAGN"
BASE COUNT          733 a         1388 c         1594 g          821 t
ORIGIN      
        1 gaaggtgacc catcatgcac gggcggatgc gaaggcgctt ctggagctgc gcgcctccat
       61 caacgagacg ctggagaagg aggagcgcgt cagcgtgacg gacatgctgg tgaagatcac
      121 ggccatggcg ctccggcaga agctcttcat gaacgtctcg ctccgcggcg accgcatcgt
      181 cgtgtttcga gaggtgcatc tgggcgtcgc cgtggcgctc gaaggatcgc tgatcgtacc
      241 ggtgatccgg aacgccgacg agaagagcct gacccgcatc agccgcgagc tgaaggacct
      301 gtcgaggagg gcgagggaga acgcgctcgc tccggaggag atctccggag gcaccttcac
      361 gctgaccaac cttggagggt accgctccac ggagttcttc acgcccgtca tcaaccagcc
      421 ggaatcggcc atcctcgggg tgggcaggac gaacgacatg cccgtcgcaa tggacggaga
      481 ggtcgcggtg cggccgatga cggcgctctc gctggcgcac gatcacaggg tcatcgacgg
      541 cgcgcctgcc gcggagttcc tcgcgctatt gatcgagctg atcgaacatc cgttccgggt
      601 cttcctctga ggtgtatgat aacaggagtc gccggcgacg ccgggaatac atggaaagag
      661 ggtgcggcat ggaatcgtgg aaggggtgcg cgtataccgc gctgattgtc ttcggatacc
      721 tggtgcgggg gaacaaggtg ctcatcgtcc gccgggccaa cgacccgtac aaggggcagc
      781 gcaccgtccc cggggggcgg aagcagcggg atgagactct gcgcgaggcg ctggtgcgcg
      841 aaatagagga agagacgggg tgcagggtgc ggagcatgga gtacgccggg atgctccacg
      901 cgatcgtcga ggggcgggat atggagtacc tctcggtcta cttcatctcg cgcgacttcg
      961 atggggagcc ccgcgacagc gaggagggga ccgtcgaatg gatggatatc gacgagagcc
     1021 tcacgatgga ggacatgcac ccgatctacc ttcgccttgt gccgtacatc ctgaagggaa
     1081 actacccgat ggagggcgcg atgcggatct ccgcggacga cgcttcggag tacatgctct
     1141 tcgggccggg ggagcacccc gcgccggaag cgcggtagac ggggcggagc agagcgcgga
     1201 cagcgccggg aagagtacag ggggagctcc cgtgcggggc tccccctgat tcgtctcatc
     1261 ccttgagcgc gtccacgatc tcgcggatgc ggtcgtccgc ctcgctgtac ggtacctggc
     1321 aggtgccgac cattctgtgg ccgccgcctc cgtgcttcag catgagcgcg ccgatgtcca
     1381 ccccgcaggt gcggttcaaa atgctgtatc cgcaggcgat ggagacgttc tgcttctgcc
     1441 tgccgtcgac gatccagacg gagacgttct gctccgggta gagactgtag atgaggaagc
     1501 ggttgcccgt gtagatgggg gagacgtccc tcaggtcggt gacgatgacg ttctgttcta
     1561 ccttcgtgtg tgtatggacc atctccgtga aacgccgcgt ctgctcgaag tagagctcca
     1621 cgcgctcctt cacgtccggc tgttcgagga tctcgccgat gcgcatgttc cggcagtggt
     1681 tgatcagctc ctccatgagc tggtagttgc tgatgcggaa ctcgcggaat ctgccgagcc
     1741 ccgtcctggg atcggtgaga aagccgagga ggatccaccc cttcggattc agtatctcct
     1801 cccgggtaag ctgccccgaa tccaccttgt ccactgcctc gatcatgtcg ccgtaggcgg
     1861 gaaacttctc cgcgccgccg tagtattcgt agatgatccg cgccgcggaa ggcgcgagtc
     1921 ggctctcgcc ctgcacgtcc ttcgaccagc cgacgcggtc gttctccgac gtatggtggt
     1981 cgaaccacat cccgcagccg gggacgtacg gtacgttcgc cagcacgtcg tcctccgtcg
     2041 gctgaaagag tccgtcctga agatccttcg ggtggacgaa ctgccacgag tcgatgatcc
     2101 ccgcctcctt gagcagcata ccgcagatca ggccgtcgaa atcagagcgt gtgatcaatc
     2161 gcatgtccta ccaactcccc gtctattttt cgcttgcaga gcgttcccca tctcttgggc
     2221 ggatcccggg tgggaccgcc gtccttttcg cgcagggctt ccgggcgtag gggccccggg
     2281 cgctctttcc gttcctgccg caaagaaagt ctgtcgggag cgcgccgtca atggtattat
     2341 tctacaacaa cgacgaggag gatgcatcaa tgaccgatat gcgccgaaaa acgaccgcgt
     2401 gtccctacga ctgcccggat gcctgcgcga tgaagggcat gttcgacggg gcgtccgtga
     2461 ccctggagcc gaatagggat ctgccgtaca ccaccttcct gtgcgggaag gggctgcgct
     2521 gggcttcgag ggcgacgagt tccgaacggc tgacgacgcc gatgctccgc agggagggcg
     2581 cgctcgtccc cgtctcctgg gatgaagcgg tggacgtctg ggcggagaag gtccgttcgg
     2641 cggtctcccg gcacgggccg ctgtcgttga tgcacctttc gagcgcaggc tcgctctact
     2701 tctcgaagct cctgctgccg cacctcttcg ccgcgctcgg cgggtacacg acgaagaagg
     2761 gaaacctctg ctcttcggcg gggagcttcg gccttgcgga gagcttcggc gaagtgcccg
     2821 tcacgcgtcc ggagtcgctt tcagaccatg cgcgcggcgt cctcttctgg gggcggaacg
     2881 cgctggagac gcacgcgcac gtcgtcccgc tgctgcagcg ggtacgaggc cgggggggcg
     2941 agatcgcctc gttagagatt cgcgagacgc cgacaacgcg cttcgccgac cggtggtggc
     3001 gggtgaaccc gggcggcgac tgggcgctgg cggcgtggct ctgcgcacgg ctcctcgagg
     3061 aaggggcgag cgacgggtgg cgggagcgcg tcgagaatcc ggaggagttc gagcgcacgg
     3121 cgcggtcgct cgacggggct ttcctgcttc gtacggcggg cgtctccgcg gagtgcgcgt
     3181 cggagcttct ggagtggctg atgcgcttcg cccccgtgac gcactacgca gccttcggcg
     3241 cgcagcggta tctgcacggc gatgcgcagt tccgctggat cggggcgctc gccgtcctcc
     3301 tgggggcctt ctcggggccg ggcgcggggc ttgccttcag caaggacgag atggcgctct
     3361 ttcctttcgc ccttctgccg aaggttgcgt atgtgcgcag gcttcccgtc gccacgtggc
     3421 cggtggagct ggagtcgctc gatccccctg tggaggcgct ctccatctcc ggtgcgaatc
     3481 ctgtgcgcca gagtccgggg agcgacttcg tgcgcgaggc gctccgcaag atccccttca
     3541 cggtctgcat cgacttcgtc ctctccgaca cggcgaagga gtgcgatctc gtgctcccga
     3601 ccacgacctt cctcgaagag gagggggact ggaagggttc ctactggcac aactacgtcg
     3661 tcaggagcga gcgcatcctc ccgccgcggg gagagtcgct ggaggagacg gagatcttca
     3721 cccgcctcgc gcgccggctt gggttgccgc tcgatctgac ggaaatgaag cgggaggtgg
     3781 accgggccat gctcgcctcg ccgctgctgg agcgtgtggg ggagggggtc ttccggtggg
     3841 acgagccgga ctactggtgc gcctcgcagc ggaaggcagc gcttcccgtg tcggttcccg
     3901 aggcggcgga gggacgggag ggcgcgctcc ggctggtcac ggtgcatcgc aaggagtata
     3961 tcaacggaca gagctgggac gccccgtccg tcgaagcgat tcctcggctc tcggtgcacc
     4021 cgctggacgc ggcgttcttt gacctgaagg agggcgacga ggccgtcgcg cgcggcagac
     4081 gacggggagc gcttcgcgtc gtcgtccggg aggacgcttc gctcgggcgg gggtactgcg
     4141 tgctcccgca ggggacggag ggcgtcaact cgctgaccga accgctggcg agccccggtt
     4201 tcggcgcgcc gttcgccgaa agctggatca cgctcggcaa ggggtagcag gtcccggtaa
     4261 ggaggtttcc cgtgcgcatg cgttttgtac tctatcttgt agcaggatat gcggtgcttg
     4321 tgacgatcgc ttttttcttc gcggacaaac tgctcttctt tccgcaccgc gagctttggg
     4381 cgacgccctc cgaacggggg cttccgtggg aagatgtgcg cttcgaggcc gcggacggaa
     4441 ccccgctcca cggctggtac gtgccgggcg cagggcgggg gccgggcggc gcggcgaagg
     4501 cgacggtgct cttctgccac ggcaacgcgg ggaata
//
LOCUS       DAOPKU010000213         4524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_20922_length_4524_cov_5.743791, whole genome shotgun sequence.
ACCESSION   DAOPKU010000213 DAOPKU010000000
VERSION     DAOPKU010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20922_length_4524_cov_5.743791"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            566..1618
                     /gene="chvE"
                     /locus_tag="PK849_07630"
     CDS             566..1618
                     /gene="chvE"
                     /locus_tag="PK849_07630"
                     /inference="COORDINATES: protein motif:HMM:NF040907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPE66034.1"
                     /translation="MKKMLLALFALVLLFSATAAFAADVLVGVAMPTQSLQRWNEDGA
                     NMKKILEGKGYKVDLQYAENNVSVQISQLENMITKGVKVMIIASIDGSALSDVLAKAA
                     EAKIPVIAYDRLIRQSPNVDYYATFDNFKVGVIQGKFIEEKLGLKDGKGPFNIEFFGG
                     SPDDNNAFFFNGGAMSILQPYLDSGKLVCHSGQTKMEQIGILAWKSEDAQARMDNLIS
                     RSYTDKKLDAVLSPNDSLAYGISASLENNGYIPGKDWPIITGQDCDRANVKNMIAGKQ
                     SMSVFKDTRALATKVAEMVDDIIQGKQPEVNDTKTYDNGMKVVPSYLLEPVFADVNNY
                     KELLLDSGYYKEEDLK"
     gene            1707..3251
                     /gene="gguA"
                     /locus_tag="PK849_07635"
     CDS             1707..3251
                     /gene="gguA"
                     /locus_tag="PK849_07635"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPE66035.1"
                     /translation="MAKTLLRMKSITKTFPGVRALNDVCFDVEEGEIHALVGENGAGK
                     STLMNILSGVYPFGSYEGEIVFDGETCVFNTIKDSEKIGIVIIHQELALVPYLSIAEN
                     VFLGNERARRGVIDWSETYARTDDLLKQIRLHENPATITKDLGVGKQQLVEIAKAISK
                     DVRLLILDEPTAALNDEDSNNLLNLLLDLKKKGITSIIISHKLNEVEKIADRITVIRD
                     GAVIETLDNATKSIREDRIIKGMVGREIVDRFPKRPPHARGDLALEVRDWTVGDPLFF
                     DRKKIKGVSFNVHKGEVVGLVGLMGAGRTELAMSLFGRSYGSDFSGCLFLGGQSATLH
                     SPKEAIARGMAYVTEDRKSAGLILIDDIRHNISLASLGKLSDKRGVLDENKEITVAED
                     LRKKMSIKTPSVFQKVGNLSGGNQQKTILARWLFANPDVLLLDEPTRGIDVGAKYEIY
                     CIINELAAMGKAIVLISSEMPEVLGMSDRIYVMNEGRFVGELDRESASQEAIMKCIMQ
                     SNREVA"
     gene            3251..4417
                     /gene="gguB"
                     /locus_tag="PK849_07640"
     CDS             3251..4417
                     /gene="gguB"
                     /locus_tag="PK849_07640"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPE66036.1"
                     /translation="MRKLKEMFMMNIRQYAMMLALLFIVLLFQYLTDGVLLRPLNLTN
                     IIQQNGYILILAIGMLLCILTGNIDLSVGSVAAFVGAVGGTLMTVWKWPVIPSILACL
                     VLGILIGCWQGYWIAYFRIPAFIVTLAGMLCFRGLTLVMLKGQTLAPMPDLFRKLFTT
                     FIPDVLGGEGLNYTALTIVAILLCAFAFFELRDRQNKKAYGFEVAPFPFWVMKILLVA
                     AVVTAFTYQLARYRGIPMVLVLLGALVLIYSFITRKTVFGRHVYAFGGNEKAAKLSGI
                     DTNKVFFLVYANMGLMAAVAGLAFMGRLNAASPVAGVNFELDAIGACFIGGASASGGI
                     GTVTGAIIGGLIMGVLNNGMSIVGVSIDWQQVVKGLVLLAAVAFDVYTKKKTAK"
BASE COUNT         1005 a         1392 c         1231 g          896 t
ORIGIN      
        1 ggcgagggag acgttgagaa tacgggcgaa aacccgcgta gagattcttt gggcaggaac
       61 gcgaagcgtc ttcgcaagaa gtgcgaagag acagcgtcgt gttcccgtac gacgtcctgt
      121 tttggacgat tgtaccatat tctttacccg cagccgcttt cctcttcttc tccgcagaat
      181 caccgatatt ccaagtccgg ctgcctggtt tctccaaagt catgcccttt ccgcaaaacg
      241 aggacttcgt tttaaaggcc gaacttcagg aggcgccggc gccgaaaaag tttttttgcc
      301 cccctcttga caagagaatc gtaaatgttc ataatatatt tattgaatca atcaattagc
      361 cttgacaaga cgaaccgcaa gccgtattct gcttagataa ggagatgatg ccctctcgag
      421 cacttcctga actggttctc tcccccgccc tgcgggggtc cgcttcacag ttcgcctccg
      481 aggcgccgga agtcctcccg gaaggaggac gcggaggggc gtaatagaag cccttcgtta
      541 ccatcttcaa aggagtgtgg tttttatgaa gaaaatgctg ctcgctcttt tcgcgcttgt
      601 ccttctcttc tccgccacag ccgctttcgc agcggacgtt cttgtgggcg tcgccatgcc
      661 cacgcagtcg ctccagcgct ggaatgaaga cggcgcgaac atgaaaaaaa ttctcgaggg
      721 aaaaggctat aaagtcgatc tgcagtacgc cgagaacaac gtcagcgttc agatctccca
      781 gctcgagaac atgattacca aaggcgtcaa ggtcatgatc atcgcctcca tcgacggttc
      841 ggctctctcc gacgtcctcg ccaaggccgc ggaagcgaag atccccgtca tcgcctacga
      901 ccgccttatc cgccagtccc ccaacgtgga ttactacgcc acgttcgaca acttcaaggt
      961 cggcgtcatc cagggcaagt tcatcgaaga gaagcttgga ctgaaagacg gcaagggccc
     1021 cttcaacatc gagttcttcg gcggttcccc cgacgacaac aacgcgttct tcttcaacgg
     1081 cggcgcaatg agcatcctgc agccctacct cgacagcggc aagcttgtct gccactccgg
     1141 ccagacgaag atggagcaga tcggcattct cgcatggaaa tccgaggacg cccaggctcg
     1201 tatggacaac ctcatctcca ggtcgtacac cgacaagaag ctcgacgccg ttctctcccc
     1261 caacgactcg ctcgcctacg gcatctccgc ctcccttgag aacaacggct acatccccgg
     1321 caaagactgg cccatcatca ccggccagga ctgcgaccgc gcgaacgtga agaacatgat
     1381 cgccgggaag cagtcgatgt ccgtcttcaa ggacacccgc gcgctcgcca caaaggtcgc
     1441 cgagatggtc gacgatatta tccagggcaa acagcccgaa gtgaacgaca cgaagaccta
     1501 cgacaacggc atgaaggtcg ttccttccta cctgcttgag cccgtcttcg ccgacgtcaa
     1561 caactacaag gaactgctcc tcgattccgg ctactacaag gaagaggatc tgaagtaatc
     1621 cgggaacact cacgcagcat atcgtgacgg aggggacggg gccatcgcgc tccgtccccc
     1681 ttgaatcagg aacaggagat gagagcatgg ccaaaacact cctgcggatg aagagcatca
     1741 cgaagacctt tcccggagtc cgcgctctga acgacgtctg tttcgacgtc gaagaggggg
     1801 agattcacgc gctcgtcggc gagaacggcg cgggaaaatc caccctgatg aacattctct
     1861 ccggcgtcta ccccttcggg tcctacgaag gggagatcgt cttcgacggg gagacctgcg
     1921 tcttcaatac gataaaagac agcgaaaaga tcggcatcgt cattatccac caggagctcg
     1981 cgctcgtccc ctacctctcg attgccgaga acgtctttct cggcaatgaa cgggcccggc
     2041 ggggcgtgat cgactggagc gagacttacg cgcgcaccga cgatcttctc aaacagatac
     2101 gactgcacga aaacccggcg actatcacaa aagatctcgg cgtggggaag cagcagctcg
     2161 tggagattgc aaaagccatc tcaaaagacg tgcgcctgct cattctcgac gaaccgaccg
     2221 cagccctcaa cgacgaggac agcaacaatc tgctgaacct gcttctggat ttgaagaaga
     2281 agggcattac gtccatcatc atctcgcaca agctcaacga agtggagaag atcgcagacc
     2341 gcatcacggt gatccgagac ggcgccgtca tcgagacgct ggataacgcg acaaaaagca
     2401 tcagggagga ccgcatcatc aaggggatgg tagggcggga gatcgtcgac cgattcccga
     2461 aacgccctcc tcacgcgaga ggcgacctcg ctcttgaagt ccgtgactgg actgtcggcg
     2521 accctctctt cttcgaccgc aaaaagatca aaggcgtcag cttcaacgtc cataagggcg
     2581 aggtcgtagg actcgtcggc cttatgggcg ccgggcggac agaactcgcc atgagcctct
     2641 tcggacgctc ctacgggagc gatttttccg gctgtctctt tctgggcggc caatcggcaa
     2701 cgcttcactc ccccaaggag gcgatcgccc gcggcatggc gtacgtcaca gaggaccgga
     2761 aatccgcagg actgattctc atcgacgaca tccgccacaa catctccctc gccagcctcg
     2821 gcaagttgag cgacaagcgg ggcgttctcg acgagaacaa agagatcacc gtggccgaag
     2881 acctgcggaa gaagatgtcc atcaagaccc cgagcgtctt tcagaaagta ggcaatctct
     2941 ccggcggaaa ccagcagaaa accattctgg cccgctggct cttcgcaaac cccgacgtgc
     3001 tgctgctcga cgaaccgacg cgcggcatcg acgtgggcgc gaagtacgaa atatactgca
     3061 tcatcaacga actggcggca atgggcaagg cgatcgtcct gatctcttcg gagatgccgg
     3121 aggtgctcgg catgagcgac cggatatacg taatgaacga ggggcgcttc gtcggcgaat
     3181 tggatcgtga gagcgcctcg caggaagcga tcatgaagtg cattatgcag agcaatcggg
     3241 aggtggcctg atgcgcaagc tgaaagagat gttcatgatg aacatccgac aatacgcgat
     3301 gatgctcgcg ctcctcttca tcgtgcttct tttccagtac ctcacggacg gcgtgctcct
     3361 ccgtccgctg aacttaacga acatcattca gcagaacggc tacatcctca tcctggcgat
     3421 cggcatgctg ctctgcattc tcaccggaaa catcgacctc tcggtcggct cggtggccgc
     3481 tttcgtcggg gccgtcggcg gaaccctgat gacggtctgg aagtggcccg tcatcccctc
     3541 catccttgcg tgcctcgttc tgggcatcct gatcggctgc tggcagggct actggatcgc
     3601 ctatttccgc attcccgcgt tcatcgtcac cctggcagga atgctctgtt tccgcggact
     3661 cacgctggtc atgctgaaag ggcagacgct cgcacccatg cccgacctct tccgtaaact
     3721 cttcaccacg ttcatccctg acgtgctggg gggcgagggg ctgaactaca ctgccctgac
     3781 aatcgtcgca atcctcctgt gcgcgttcgc cttcttcgag ctgcgggatc gtcaaaacaa
     3841 aaaagcgtac ggattcgagg tcgccccgtt ccccttctgg gtgatgaaga tcctgctcgt
     3901 cgccgcggtc gtcacggcct ttacctacca gctcgcccgg taccgcggaa ttccgatggt
     3961 actggtgctc cttggagcgc tcgtccttat ctacagcttc atcaccagga agaccgtctt
     4021 cggccgtcac gtctacgcgt tcggcggcaa cgagaaggcg gcgaagctct cgggtatcga
     4081 tacgaacaaa gtctttttcc tcgtctatgc gaacatgggc ctgatggcgg cggtcgcggg
     4141 acttgccttc atgggacggc tcaacgcggc ctctcccgtc gccggggtca atttcgagct
     4201 tgacgccatc ggcgcgtgct tcatcggcgg cgcatccgcc tccggaggca tcggcaccgt
     4261 cacaggggcc atcatcggcg gactgatcat gggcgtgctc aacaacggca tgtccatcgt
     4321 aggcgtctcc atcgactggc agcaggtggt caaaggactc gtcctcctcg ctgcggtcgc
     4381 cttcgacgtc tacacgaaga agaaaaccgc gaagtagctc gcagtacagc ggcccggcgc
     4441 tccccttttt tgggcgaacc gggccgcttc ccgattgtga aggaggactc ttcatgagcg
     4501 aacactgttc ccccgcccct tccg
//
LOCUS       DAOPKU010000214         4506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_21039_length_4506_cov_4.342844, whole genome shotgun sequence.
ACCESSION   DAOPKU010000214 DAOPKU010000000
VERSION     DAOPKU010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21039_length_4506_cov_4.342844"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PK849_07645"
     CDS             complement(<1..197)
                     /locus_tag="PK849_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE66037.1"
                     /translation="MLRILLADDHPLTRSGLAAWLEAEEGVELVGQAVDGESAWTMLL
                     ETQPDLALLDIEMPNGSGIEIA"
     gene            438..962
                     /locus_tag="PK849_07650"
     CDS             438..962
                     /locus_tag="PK849_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66038.1"
                     /translation="MKKTTIFALVAVLVLVAASGAFAWGGRGGRGMVAPGTEGTTAPM
                     MQRGMAAPGAPGGTAVPMMGRGMAGRTANGRGAMMGNQQMMKGMRGRGASGGMGGRGM
                     MSVWANVEIPQEIRDKQVEMQKLSVDMRAEMQKKPVDRAKVEELYKKRVELRDALSGW
                     RMKTRLDTIEKLQK"
     gene            1206..>2031
                     /locus_tag="PK849_07655"
     CDS             1206..>2031
                     /locus_tag="PK849_07655"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPE66039.1"
                     /translation="MRDLLLEFVTEEWVDGLDLDTLDRVHDSFVTDDLRERFDDIIWR
                     VSFRGRMVYLCLIVEFQSSPDRWIAGRLMAYIGLLYGDIVKRNEIVDGFFPPVLPIVL
                     YSGEGRWTVPLSLEEMLHPASSVLGRYAPHLRYLLLDESAYSEERLAQSKNLAAFLFR
                     MENSRTPDHLLEIVRSLGGVLDGEEHRHLRRSFAVFVRNALLPKKTRRRNFPDVEDLV
                     EVERMIAEGTRNWADGWIKEGEERGEKRGEERGEKRGEERGEKRGEQRGRERTALRM"
     assembly_gap    2032..2131
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2239..2421
                     /locus_tag="PK849_07660"
     CDS             2239..2421
                     /locus_tag="PK849_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66040.1"
                     /translation="MPCNWADGWIKEGEERGEKRGRERTALRMLEHGMDVALIAKLAE
                     LSEERVRRLAAQSSSQ"
     gene            complement(2581..3339)
                     /locus_tag="PK849_07665"
     CDS             complement(2581..3339)
                     /locus_tag="PK849_07665"
                     /inference="COORDINATES: protein motif:HMM:NF006702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HPE66041.1"
                     /translation="MKPLFDLHTHTLASGHAYSTLMENIEAASERGLAAMGFSEHAPT
                     MSGAPDPMYFMNFKIIPDTIMGVRIYRGIEANILDFNGAVDVDAAMASRLDYVIASLH
                     PPIISSGTREENTRALVGAMRHPFVTIIGHPDDDRYPLDYAELAAAAAREHVALELNN
                     SSFRPSTGRVNGRKNAVNLLNACRIHDARVVMNSDSHIAFDVGDLSLCEEIIEETNFP
                     RELVLNYTLEGLEYVLKEGVSLPDCEKLLCASGK"
     gene            complement(3354..>4506)
                     /locus_tag="PK849_07670"
     CDS             complement(3354..>4506)
                     /locus_tag="PK849_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66042.1"
                     /translation="HWFGRTRALDDIRLEIGASEFVAVLGPSGCGKTTLLRILGGFLR
                     PTEGTVTFGDDVYSSPLVHVPVERRDLGMVFQSFALWPHMTVRRHVEFPLKSPRHKGT
                     SEREKHEAVERTLEVTGLTSLQHRLPGELSGGQRQRVSLARAIVSKPSILLMDEPLSA
                     LDQELRIEMRREIQDIHRLTGATIVYVTHDQGEALAMADRLVIMKDGRIEQAGTPREV
                     YLHPRTPFAAGFVSKCNFVKGTWTGDRFAVQEDPSLLFDGAGVAEVFRGMNVYPVRPE
                     QFRIAARGRGLEGKIANKQYNGREIHYAVRCQGTIFTVYADAFEDYRPGDSVRLSFEG
                     TVRDDREDGLDREAFMVYNRPAHTDSTAGERAPVPHSGEAAETPRLGEQ"
BASE COUNT          817 a         1254 c         1496 g          839 t
ORIGIN      
        1 gcgatctcaa tgccgctgcc gttgggcatc tcaatgtcga gcagcgcaag gtcgggctgc
       61 gtttcaagca gcatcgtcca cgcggactct ccgtccacgg cctgtccgac gagctcgacg
      121 ccctcctcgg cctccagcca ggccgcgagg ccggagcgtg tgagcggatg gtcgtcggcg
      181 agcagtattc gcagcatctc ggcgtccccc tttcaaaagc cgcgttccgc ggtatgcggc
      241 gcggcgtgaa gattctctcc ctatagtata tccgtttttt gaaccgaacg aagaaagaat
      301 acgttcttcg ggtttcttcg gaaattgtaa cttgttacag tcgggagagc ggtagttcgg
      361 accatgcgcg tccggccgcg ccgcggtatc ctgtacccgt cacatcaaaa cacacttttg
      421 aaaggcgggg ttcactcatg aaaaagacaa cgatattcgc gttggtggcg gttctggtac
      481 tggtagcggc aagcggagcg ttcgcgtggg gcggacgggg aggcagagga atggttgctc
      541 ccggaaccga aggaacgacg gcgcccatga tgcagagagg tatggccgct cccggcgcac
      601 ccggcggcac ggcggttccg atgatgggaa gaggcatggc cggacgtact gcgaacggac
      661 ggggcgccat gatgggcaat cagcagatga tgaaagggat gcgcggtcgc ggagcgagcg
      721 gcggcatggg cggacgcggc atgatgagcg tgtgggcgaa cgtcgagatt ccgcaggaga
      781 ttcgcgacaa gcaggtcgag atgcagaaac tctcagttga tatgcgcgcc gagatgcaga
      841 agaagccggt cgaccgcgcc aaggtcgagg agctgtacaa gaagcgcgtg gagctgcgcg
      901 acgccctttc cggctggcgg atgaagacaa ggctcgacac gatcgaaaag ctccagaagt
      961 aagcgaaaaa acagcgcgcc tcccgaaagc agcgctgcaa cgaagagagg gcgaggactc
     1021 cggtccccgc cctctcttgt gcgaacgtat ccggcacggc caaggtgcga aagcgtgcga
     1081 gcggtctctt gaagcggtta agaagcatgg cgcccttcgc ttgcggattc ttgccgccga
     1141 ttctataatg ttcgtatgga actcacgaca gaggatacaa gcttctcttt tcgaataaag
     1201 agatgatgcg cgaccttctc cttgaattcg tcacggagga gtgggtcgac ggtctggatt
     1261 tggatacgct ggacagggtg cacgattcct tcgtgaccga cgatttacgg gagcggttcg
     1321 acgacattat ctggagggtg agcttcagag ggagaatggt ctatctctgt ctgatcgtcg
     1381 agttccagtc ctcgccggac cggtggatcg ccggacggct tatggcgtat atcggtttgc
     1441 tctacggcga tatcgtcaaa cggaacgaga tcgtcgacgg ctttttccct ccggtgctgc
     1501 cgatcgtgct ctattcgggg gaggggagat ggaccgttcc cctctctttg gaggagatgc
     1561 tgcacccggc ttcgtcggtt ctcgggcgct acgctccgca tttgcgctat ctgcttctcg
     1621 acgagagcgc gtacagcgag gagcggctcg cgcagtcgaa gaatctggcg gcgtttcttt
     1681 ttcgcatgga gaacagcagg acgcccgatc acctgctgga gatagtgcgg tcgctcggcg
     1741 gcgtgctgga tggagaggaa cacaggcatc tgaggcgctc gttcgcggtt ttcgtgcgga
     1801 acgcgctgct gccgaagaag acccgcagaa ggaattttcc cgatgtggag gacctggtgg
     1861 aggtggagag aatgatagcc gaaggaacgc gcaactgggc cgacgggtgg atcaaagaag
     1921 gcgaagagag aggcgaaaag aggggcgaag agagaggcga aaagaggggc gaagagagag
     1981 gcgaaaagag aggcgaacag agaggacgcg agcgtacggc gctccgcatg cnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggagcacg gaatggatgt ggcgctcatc
     2161 gccaagcttg ccgaactttc ggaggaacgc gtccgccagc tcgctgcgca gtcctcgcct
     2221 cactgaggag aaatgagcat gccctgcaac tgggccgacg gctggatcaa ggaaggcgaa
     2281 gagagaggcg aaaagagagg gcgcgagcgt acggcgctcc gaatgctgga gcacggaatg
     2341 gatgtggcgc tcatcgccaa gcttgccgaa ctttcggagg agcgcgtccg tcggctcgcc
     2401 gcacaatcct cgtctcaatg attgctcgaa cttcatcgag cgctccgttg tctcaaacca
     2461 gtaaaaagtg aaagccgctg ttttttcaac aacgaaaacg gcggctttct tcgattcttg
     2521 aagcggagcc tgtcccccga cccgagatga gtcgtacccg cttgcagcga atgccccgct
     2581 ttatttcccc gacgcgcaga ggagcttttc gcagtcgggg agcgagacgc cctccttgag
     2641 cacgtattcc aggccttcca gtgtgtagtt cagcacgagt tcgcggggga agttcgtctc
     2701 ttcgataatc tcctcgcaga gcgacaggtc gccgacgtcg aaggcgatgt gggagtcgct
     2761 gttcatgacg acgcgggcgt cgtggatgcg gcaggcgttc agcagattga ccgcgttctt
     2821 tcgtccgttc acccggcccg tgctcgggcg gaagctggag ttgttcagct ccagcgccac
     2881 atgctctctt gcggccgcgg cggcgagctc cgcgtagtcg agcggatagc ggtcgtcgtc
     2941 ggggtggccg atgatggtca cgaaggggtg tcgcatcgcg ccgacgaggg cgcgggtgtt
     3001 ctcttccctc gtccccgacg agatgatggg ggggtgcaga ctggcgatga cgtagtccag
     3061 ccgcgaggcc atggccgcgt ccacgtcgac ggcgccgttg aagtcgagaa tgttggcctc
     3121 gatgccacgg tagatgcgga cgcccatgat ggtgtcgggg atgattttga agttcatgaa
     3181 gtacatgggg tcgggcgcgc ccgacatcgt gggggcgtgc tcggaaaagc ccatcgcggc
     3241 gagtccccgt tcgctcgcgg cttctatgtt ctccatcagc gtgctgtagg cgtgcccgct
     3301 ggccagtgtg tgcgtgtgca ggtcaaaaag cggtttcatg tgatctccct ttttcattgt
     3361 tctccaagcc ggggcgtttc ggcggcctct cccgaatgcg ggacgggcgc tcgttccccg
     3421 gcggtgctgt ccgtgtgcgc cggtctgttg tacaccatga aggcctccct gtccagtccg
     3481 tcctcccggt cgtcccggac ggttccctcg aaggagaggc ggacggagtc tccggggcgg
     3541 tagtcctcga aggcgtcggc gtagacggtg aagatggtcc cctggcagcg taccgcgtag
     3601 tgaatctccc ggccgttgta ctgcttgttg gcgatcttcc cctccaatcc ccgtccccgg
     3661 gcggcgatgc ggaactgctc ggggcggacg gggtagacgt tcattccgcg gaagacctcc
     3721 gcgacccccg cgccgtcgaa gagcagcgac gggtcctcct gcacggcgaa ccggtccccg
     3781 gtccatgttc ccttgacgaa gttgcacttg ctgacgaatc cggcggcgaa gggcgtcctc
     3841 ggatggaggt agacctccct cggcgttccc gcctgttcga tgcgcccgtc cttcatgatg
     3901 acgagccggt cggccatggc gagcgcctcc ccctggtcgt gggtgacgta gacgatggtc
     3961 gcgccggtga gcctgtggat gtcctgaatt tcgcggcgca tctcgatccg cagctcctgg
     4021 tcgagggcgc tcagcggctc gtccatcagc aggatggagg gcttggagac gatggcccgg
     4081 gccagggaga cgcgctgccg ctgccctccg gagagctccc ccggcaggcg gtgctgcagc
     4141 gacgtcaggc ctgtaacctc aagggtccgc tccacggcct cgtgcttctc gcgttcgctc
     4201 gtccccttgt ggcgggggct cttcagcgga aactcgacgt ggcggcgcac cgtcatgtgc
     4261 ggccagagcg cgaacgactg aaagaccatg ccgaggtcgc gccgctccac gggtacgtgg
     4321 acgaggggcg aggagtagac gtcgtcgccg aaggtcaccg tgccctccgt cggccggagg
     4381 aagccgccga gtatgcggag cagcgtcgtc ttgccgcagc cgctcggtcc gaggaccgcg
     4441 acgaactcgg acgctcctat ttcgagccgt atgtcatcga gcgcccgcgt ccggccgaac
     4501 cagtgg
//
LOCUS       DAOPKU010000215         4434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_21489_length_4434_cov_3.689198, whole genome shotgun sequence.
ACCESSION   DAOPKU010000215 DAOPKU010000000
VERSION     DAOPKU010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21489_length_4434_cov_3.689198"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PK849_07675"
     CDS             <1..572
                     /locus_tag="PK849_07675"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPE66043.1"
                     /translation="SASWERSVNMMRTAYVHIAQVRGDLPVEVRGISWYGYGAPDTTY
                     ITPLWPVMSKLPPLYGIGDRYHDYDPKSGWWVNTRVQELAGLRYQDARKEIHAARDAK
                     LNPLYVQTRMIQDKAAEMLKAGDKDGAVSLITDFAYAHAVDWNQRWLALGDRLLSKYA
                     LGYTDVKTTPYPEEWNEIVGFDTPKRSTK"
     gene            672..1577
                     /gene="pgsW"
                     /locus_tag="PK849_07680"
     CDS             672..1577
                     /gene="pgsW"
                     /locus_tag="PK849_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HPE66044.1"
                     /translation="MKLFRTLLFFILLATPADASTIEHGPAARLMARCEAVLYEEKCR
                     LGLYDALTDPEKTALIGVEFSPLTTSQGHLPDKQASARPAMADAVAEYLRRAGVGEGD
                     WVAVNASASFPGFILASLCAVESMQANAQIAFSYGASMYGGTDPEFTFPVMLDLLNSE
                     GLLSTRLAAVAPGGAGDRMAETLLEDPRPTVDLLLASRSEKRIDAPDVAEAIRRRLEI
                     FDDTPRPVKCFISCGGPVVALGTTEVVLELPHGLITRFEPIPKDPERGLLFDFLDRGT
                     PVLHLLFVRGICEDFGIPYERVAGQ"
     gene            1788..3344
                     /locus_tag="PK849_07685"
     CDS             1788..3344
                     /locus_tag="PK849_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPE66045.1"
                     /translation="MERTSAERRNLFTKTGGLLAGIAAALLMASLFAFMPGTGEAATW
                     TVTNGGDGGTGALRSVIESAAAGDSVVFDSSVSIVNLNTYLFVNKALTITGPVTIKQN
                     GAGQRVLYVTGDCTFNNLTITGGNLGDPNSNGGAGIYNYSTLTMNNCTVTGNTGGGGI
                     LNKQNLTMNSCTISGNAASGHLKAGGIASTATGSWEVSLTMTDCIVENNIGSDRANSG
                     GIYNGVDGTLVMTRSTVRNNECSSLGGGMYISAYSSTTLKNRSVVTGNRPDQICCGYG
                     ASYTTDGTCTIGGASGTSATALSGFAEGASPSPRKTTGEPDVAAVERDLRSPSSELFG
                     AVKNLLESDLGGLPGDVSAGLSSIDATLYNAFAYEDVPLADASGDGELVIEFTASWPQ
                     NVRYYAALAEWEDAAAGALAVKEYVIPERGVQFEIKPGQPLPDGVTPPDFYEEGEGLM
                     TWRNVIADNGPYDHNRQVGIVTFRVTSIRAEARTAAAASGGSGCSTGAASPFALLLGA
                     PLLLLRKKHP"
     gene            complement(3395..>4434)
                     /locus_tag="PK849_07690"
     CDS             complement(3395..>4434)
                     /locus_tag="PK849_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPE66046.1"
                     /translation="ELRSRLLRAIGGLLLVLIALLPFANKLYAWLAQPLLDKLPAGGR
                     LIATQVASPFFAPMKLALCVALLLRRVSFAGMLALVCSTVIGAGFLCGSLSVLFFGEE
                     ATALALTVPSPAAFGETLVLLFWAVIGWEILGNYTLEIESPKRSIPLAAGIAFCAICA
                     VYLAIAAALPRIGAGGTIPSMTDLLTPLFGSFASPLLAVFGILLCVCTYLMVVGGVSR
                     LVASMAERGELPRMLALRNKEGAPSRAIAALGCVHGAGLLLSSLSVLDVASLVGAANG
                     LFLLNSLLAVAAAARLLRSPAVRTASAVLAACFLILFALADRTALLVSVTVLLFSLKE
                     ASRGDRVAVSG"
BASE COUNT          904 a         1499 c         1388 g          643 t
ORIGIN      
        1 cctccgccag ctgggagcgc tccgtcaaca tgatgcggac ggcctacgtc cacatcgccc
       61 aggtccgcgg cgatctcccc gtggaagtgc gcggcatcag ctggtacggc tacggcgcgc
      121 cggacaccac ctacatcacc cccctgtggc ccgtgatgag caagctgccc ccgctctacg
      181 gcatcggcga cagataccac gactacgatc ccaaatccgg ctggtgggtg aacacccgcg
      241 tgcaggaact cgcaggactt cgctaccagg acgcccgcaa ggagatccac gccgcgcgcg
      301 acgcgaagct caatccgctc tacgtgcaga cgcggatgat ccaggacaag gcagccgaga
      361 tgctgaaggc cggcgataag gacggcgcgg tctccctgat caccgacttc gcctacgccc
      421 atgccgtcga ctggaaccag cgctggctcg ccctcggcga caggctgctg agcaagtacg
      481 ccctcggcta caccgacgtg aagaccacgc cgtaccccga ggagtggaac gagatcgtcg
      541 gcttcgacac cccgaagcgc tccacgaaat agtaattccc tgttgatgaa agggcgcgga
      601 gggcttgaga gccgccgcgc ccttttcgta ttcgcgcggc gccccgccgc gagactcgag
      661 gaggcgaacg tatgaagctc ttccgaacgc tgctgttttt cattctgctg gcaacccccg
      721 ccgacgcttc caccatcgag catgggccgg cggctcgcct gatggcgcgc tgcgaagccg
      781 tcctctacga ggaaaagtgc aggctcggcc tgtacgatgc gttgacggac ccggaaaaaa
      841 ccgccctgat cggcgtcgag ttctcgcccc ttacgaccag tcaggggcat ctgccggaca
      901 aacaggcgtc ggcccgcccc gccatggcgg acgccgtcgc ggagtacctc cggcgggcgg
      961 gcgtcgggga aggggactgg gtcgccgtga acgcgagcgc ctccttcccc ggcttcatcc
     1021 tcgcctcgct ctgcgccgtc gagagcatgc aggcgaacgc gcagatcgcc ttctcctacg
     1081 gcgcctccat gtacggcgga accgaccctg agttcacctt tcccgtcatg ctcgacctcc
     1141 tcaacagcga gggacttctt tcgacgcgcc tcgcggccgt ggcccccggc ggggccggag
     1201 accgaatggc ggagacgctg ctggaggacc cgcgccccac cgtcgacctc ctgctggcct
     1261 ccaggagcga aaagcgcatc gacgcgccgg acgtcgccga agccatccgt cgccgcctcg
     1321 aaatcttcga cgacacgccg cgccccgtga agtgcttcat cagctgcggg ggacccgtcg
     1381 tcgccctggg aacgaccgag gtcgtactgg aactccctca tggcctgata acacgcttcg
     1441 agccgatccc gaaagacccc gaacgcggcc tgctcttcga cttcctcgac agaggaaccc
     1501 ccgtgctcca cctcctcttc gtccgcggca tctgcgagga tttcggcatc ccgtacgagc
     1561 gcgtcgcggg gcagtagcct ctttcagaac gccgccccgc gcttcgggca agaataccgt
     1621 aataaaaaac tccgaaaaat cgaaattccc tcttgactct cacacagtgg aaaggtttat
     1681 gatacctcgc gtacgcacca ctattccggc ggcatgtcga cgacggcgca aaaacgtacg
     1741 ttcatcgacc gcgcggagta tattccacga gaaagaagga agacgcgatg gaacggacaa
     1801 gcgcagaaag acggaatcta ttcaccaaaa caggaggtct cctcgccgga atcgcggcgg
     1861 cactcctcat ggcgtcgctg ttcgccttta tgccggggac gggagaggcc gcgacatgga
     1921 cggtgacgaa cgggggcgac ggcggaacgg gcgccttgag gagcgttatc gagagcgccg
     1981 cggccggcga ctcggtcgtt ttcgattcgt ccgtctccat cgtcaatctg aacacctacc
     2041 tcttcgtcaa caaggcgctc acgatcaccg gtcccgtcac tatcaagcag aacggcgccg
     2101 ggcagcgcgt cctctacgtc acgggcgact gcacgttcaa caacctgacg atcaccggcg
     2161 gcaacctcgg cgacccgaac agcaacggcg gcgccgggat atacaactat tcgacgctga
     2221 cgatgaacaa ctgcaccgtg accggaaaca ccggaggcgg cggcatcctg aacaaacaaa
     2281 acctcaccat gaacagctgt acaatcagcg ggaacgcggc ttcaggacac cttaaagccg
     2341 ggggaatcgc cagcaccgcc accggctcct gggaggtgtc tctgacgatg accgactgta
     2401 tcgtggagaa caatatcggc tcggacaggg caaattcggg aggtatctac aacggtgtcg
     2461 atggaacgct cgtcatgacg cggagcacag tcagaaacaa cgagtgcagc agcctcggcg
     2521 gagggatgta tatcagcgcc tattcctcga cgacgctgaa aaaccgcagc gtcgtgaccg
     2581 gcaatcggcc ggatcagata tgctgcggct acggcgcatc ctataccacc gacggtacct
     2641 gcaccatcgg aggcgcttcc ggaaccagcg cgacggcgct ctccggattc gccgagggag
     2701 cttccccctc gcctcggaag accacgggcg aaccggacgt cgcggcggtc gagcgggatc
     2761 tgcgctcgcc ctcgagcgaa ctcttcggcg ctgtgaagaa tctcctggag tccgacctcg
     2821 ggggacttcc cggagacgtc tcggcggggc tttcgagcat cgacgcgacg ctgtacaacg
     2881 ccttcgccta cgaggacgtg ccgctcgcgg acgcatcggg agatggcgag ctggtgatcg
     2941 agttcaccgc gtcgtggccg cagaacgtcc gctactacgc cgcgctggcg gagtgggagg
     3001 atgccgctgc gggagctctc gccgtgaagg agtacgtcat ccccgagcgc ggcgtccagt
     3061 tcgagattaa gcccggtcag ccgctccccg acggcgtgac gcctcccgac ttctacgagg
     3121 agggcgaagg attgatgacc tggcgcaacg tgatcgcgga caacggcccg tacgaccaca
     3181 accgacaagt cggcatcgtg accttccgcg tcacctccat ccgcgcagag gcccggacgg
     3241 ccgccgcggc atccggcggt tccggctgct cgacgggcgc cgcctcgccc ttcgcgctcc
     3301 tgctgggggc gccgctgttg ctcctacgga aaaaacaccc gtaaaacacg aagggggcgc
     3361 gagaaaagcg ttttctcgcg cccccttcga gcattcatcc ggagacggcc acccggtcgc
     3421 ctcggctcgc ctctttcaaa gagaagagca gcactgtcac cgagaccagc agcgccgtcc
     3481 tgtcggcgag agcgaagaga atcaggaagc acgcggccaa gacggcagag gccgtccgga
     3541 cggccggaga gcgcagcagg cgagccgccg ccgcgaccgc cagcagcgag ttcagcagaa
     3601 agagtccgtt ggcggcgccc accagagaag cgacgtccag cacagagagg gaggagagca
     3661 gcagccccgc cccgtggacg cacccaagag ccgctatcgc tctggacggc gcgccctcct
     3721 tgttcctgag ggcgagcatg cggggaagct ctccgcgctc ggccatcgac gcgacaaggc
     3781 gggagactcc tccgaccacc atgaggtacg tgcagacgca gagcaatatg ccgaacaccg
     3841 cgagcagcgg agaggcgaaa ctcccgaaga ggggcgtcag cagatccgtc atcgagggaa
     3901 tggttccgcc cgccccgatg cgcggcaacg cggcggctat cgcgagatac actgcgcaga
     3961 tcgcgcagaa ggcgatcccc gccgccaggg gaatgctccg cttgggcgac tcgatttcga
     4021 gcgtgtagtt ccccagaatc tcccacccga tcacggccca gaagaggagc accagcgtct
     4081 ccccgaaggc agcgggggac ggaacggtca gcgcaagcgc cgttgcttcc tccccgaaga
     4141 agaggacgga gaggcttccg cagaggaagc ccgccccgat gacggtggaa cacacaaggg
     4201 ccagcattcc cgcgaaggag acccgccgca gcagcagcgc gacgcagagc gccagcttca
     4261 tcggcgcgaa gaacggcgag gcgacctggg tggcgatcag gcgaccgccc gcgggcaact
     4321 tgtcgagcag cggctgcgcc agccaggcgt acagcttgtt cgcgaacggc agcagcgcga
     4381 tgagcacaag gagcaagccg ccgatcgcgc gcagcaggcg cgagcgcagt tcga
//
LOCUS       DAOPKU010000216         4415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_21608_length_4415_cov_4.454358, whole genome shotgun sequence.
ACCESSION   DAOPKU010000216 DAOPKU010000000
VERSION     DAOPKU010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21608_length_4415_cov_4.454358"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PK849_07695"
     CDS             complement(<1..679)
                     /locus_tag="PK849_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE66047.1"
                     /translation="MVEQNGPAPLVEVEGMVKSFGENAVLRDVSLALRRSDVMAVVGG
                     NGAGKSTLMKILAGIYTPDKGSIRIEGSDVSLHTPSAAHAMGVYLVPQEPLLFPNMTV
                     EENLSLGLDEPRTDVRKRAVALIKRLNWPLSMSRQANSLSIAEQQLVEILRGLLRSAK
                     VLILDEPTSSLTFAEIQSLFKLIKELRAEGVGIFYITHRLDEVFEIATHVAILRDGRV
                     TLEGPVGG"
     gene            complement(862..1197)
                     /locus_tag="PK849_07700"
     CDS             complement(862..1197)
                     /locus_tag="PK849_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE66048.1"
                     /translation="MFKVNESEFEYRFGDSGPKYLMRGPRMNFGVVVLQAGQDFRDHL
                     HNVMEENFFILEGELEFRVADQVLTAKAGDLVHVEPGEAHYLRNASDAPAKAVFCLAP
                     FQEKDKVDL"
     gene            complement(1215..2783)
                     /gene="lsrK"
                     /locus_tag="PK849_07705"
     CDS             complement(1215..2783)
                     /gene="lsrK"
                     /locus_tag="PK849_07705"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: protein motif:HMM:NF008187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HPE66049.1"
                     /translation="MASGKYLMAIDAGTGSARAVLFDESGRQVAVSQAEWSHKEDPRF
                     PGSMDFDVEGNWKLIVGCVKGVLEKSGADPRSVAAIATTSMREAIVLYDGAGKEIWAC
                     ANVDARSSAEVVKLMRLRPGIEEELYALSGQTFSLNALPRLLWVKENLPDVYESVRAV
                     NMLNDWIAYKLSGVLTAEPSNGCTSGIFDIAARKWKPEIAASCGLRTDIYPAVTESAE
                     IIGRVSADCAALTGLSTETLVVSGGGDAQLGCVGVGAVADGQAAVLGGSFWQYEFNTA
                     VPVMDPKCRVRVNCHAVPGVFQYEAIAFFPGLVMRWFRDAFCQEEKRVEAEGGPDAYM
                     QLAEGASKIPAGSHGMMCAFSDVMNYIAWKHAAPTFSNFSLDAEKFNKYTFFRSLMEN
                     AALVTRGHLQLVEEVTGRRPEGIVFANGASKSPVWSQIMADVLGLTVRVPEVKEATAL
                     GAALCAGVAAGVYDNMEDAVRRTVRWDASYEPVTENRKVYDDLYDKWRAMYGAQLKLA
                     DEGVTAHMWRAPGV"
     gene            3062..4243
                     /locus_tag="PK849_07710"
     CDS             3062..4243
                     /locus_tag="PK849_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPE66050.1"
                     /translation="MHDIQRKAQAMQERIVQWRRALHAMPEVGFDTPGTERFIVEELQ
                     KMGVTEIRHGDGKKGIVALVQGKKPGGVLGIRADYDALHMTEETGLPFAATNGNMHAC
                     GHDAHAAILLGTANLLLDCADELAGTVKLIFQPSEENGKGALAMIKDGVLEEPKMDAI
                     IGLHIGSLWKGLNSGEVGYRYGALMAAADWFKITFGGKGGHGATPHLTVDPLAMACQT
                     VGLLQQIVSRETSPLDSAVVTVGTIQGGTMPNIIAPSCTIAGTVRCLSPEVRNHLETR
                     IREICTDTARALRGSVAIEYTYGPPPLLNDRAVTDELKLAMEQIVGAEKVREVEQPTM
                     GGEDMAFFQEHVPGSFFFLPASFEDESRNFPHHHPKFDLNEDVFWVGPAVMVGFALSR
                     G"
BASE COUNT          828 a         1477 c         1347 g          763 t
ORIGIN      
        1 agccgccgac cggcccttcg agggtcaccc ggccgtcgcg caggatggcc acgtgcgtcg
       61 cgatctcgaa gacctcgtcg agcctgtggg tgatgtagaa gatccccacg ccctcggcgc
      121 ggagctcctt gatcagcttg aagagcgact ggatttcggc gaaggtgagc gaggaggtcg
      181 gctcgtcgag gatgaggacc ttcgcggagc gcagcagccc gcgcagtatc tcgacgagct
      241 gctgctcggc gatggagagg gagttggcct gtcgcgacat cgagagcggc cagttgagac
      301 gcttgatcag cgcaacggcg cgcttgcgga cgtcggtcct gggttcgtcg agcccgaggc
      361 tcagattctc ctccaccgtc atattgggga agagcagcgg ctcctggggg acgaggtaca
      421 cccccatcgc atgcgccgcc gaaggggtgt gcagcgagac atcgctcccc tcgatgcgga
      481 tgcttccctt gtccggcgtg taaataccgg cgaggatctt catcagcgtg ctctttccgg
      541 cgccgtttcc cccgactacc gccatcacgt ccgaacgccg gagcgccaga gagacgtcgc
      601 gcagcacggc gttctctccg aaggacttga ccatcccctc cacttcgacg aggggggcgg
      661 gcccgttctg ttccaccata cgatcacccc gcagattcta ttgcgtttcg ccgcatcgcc
      721 ggaatgcgcc gccggaagag aaaccgtcct ctcccgaacc gccggcttgc gaaaaagggc
      781 gttgtccccg agcgtccttc aagtcaatgc ccacgccgcg cggcgagctc ccgaaaggag
      841 acggccgcct actctcgtga atcagagatc gactttgtcc ttctcctgga acggcgcgag
      901 gcaaaagacc gccttcgcgg gcgcgtccga ggcgttccgc agatagtggg cctcgcccgg
      961 ttcgacgtgc accaggtcgc ccgctttggc cgtcagcacc tggtcggcaa cgcggaactc
     1021 cagctcccct tcgaggatga agaagttctc ctccatgacg ttgtgcagat gatcccggaa
     1081 gtcctgcccc gcctggagca ccaccacgcc gaagttcatg cgggggcctc tcatcaggta
     1141 cttggggccg ctgtcgccga accggtactc gaattcgctc tcgttgacct tgaacattag
     1201 ctcataccct ccattcagac gccgggcgcc cgccacatat gcgcggtcac gccctcgtcg
     1261 gcaagtttca gttgcgcgcc gtacattgcg cgccatttat cgtacagatc gtcgtacacc
     1321 ttccggtttt ccgtaacggg ttcgtaggat gcgtcccacc ggaccgtccg gcggacggcg
     1381 tcctccatat tgtcgtacac gcccgccgcc acgcccgcgc agagcgccgc gcccagggcg
     1441 gtcgcctcct tcacctcggg aacccgcacc gtgagcccga ggacgtcggc cataatctga
     1501 gaccagaccg ggcttttgga ggcgccgttc gcgaagacga ttccttccgg gcgccttccc
     1561 gtgacctcct caacaagctg cagatgaccg cgcgtcacga gcgccgcgtt ctccatcagg
     1621 gagcggaaga aggtgtactt gttgaacttt tcggcgtcga gcgagaagtt ggaaaaggtg
     1681 ggggccgcgt gcttccacgc gatgtagttc ataacgtcgg agaaggcgca catcatcccg
     1741 tgcgatcctg cggggatctt cgaagcgccc tcggcgagct gcatgtaggc gtcggggcct
     1801 ccttcggcct ccacgcgctt ctcctcctgg cagaaagcgt ctcggaacca gcgcatgacc
     1861 aggcctggga agaaggcgat ggcctcgtac tggaagacgc ccggcacggc gtggcagttc
     1921 acccgcacgc ggcatttcgg gtccattaca gggacggcgg tgttgaattc gtactgccag
     1981 aagctcccgc cgagcacggc ggcctgtccg tccgccaccg cgcccacgcc cacgcacccg
     2041 agctgcgcgt cgccgccgcc cgagacgacg agcgtctccg tgctcaagcc cgtgagggcg
     2101 gcgcagtccg cgctcaccct gccgattatc tcggcgctct cggtcaccgc ggggtagatg
     2161 tcggtgcgca acccgcacga cgcggcgatc tccggcttcc acttcctggc ggcgatatcg
     2221 aaaatgccgc tggtgcagcc gttggagggc tccgccgtca gcacgccgga gagtttgtag
     2281 gcgatccagt cgttcagcat gttcacggcg cggaccgact cgtagacgtc cggaaggttc
     2341 tccttcaccc agagcagcct ggggagcgcg ttcagcgaga acgtctggcc ggagagcgcg
     2401 tagagctcct cctctattcc cggccgaagg cgcatcagct tcacgacctc cgccgaggac
     2461 ctggcgtcca cgttggcgca ggcccatatc tccttgcccg ccccgtcgta gaggacgatc
     2521 gcctcgcgca tgctggtggt ggctatcgcc gcgaccgacc gggggtcggc gccgctcttc
     2581 tcaagcacgc ccttgacgca cccgacgatc agcttccagt tcccctccac gtcgaaatcc
     2641 atcgagccgg gaaaccgggg atcctccttg tggctccact ccgcctgcga caccgcaacc
     2701 tgccgtccgg actcgtcgaa gagcacggcc cgcgcgctgc ccgttcccgc gtcgatcgcc
     2761 atcagatact tacccgaagc caaagcgctc accccctgga aagaatcggc gtctctaatc
     2821 acatatgtga agtcatctca tatatgtgag aaagtctcta cggatgccat tataggaaaa
     2881 atggtacagt attgtcaatg caaattcggt tttcttgcgg aaagcggcgg cttcccgagg
     2941 caaccccgga cgcccgcgga acatcttgga atccccgccg ctccatggta tcatctgctc
     3001 acacgagcat tacgaataca gaataggaac atgaacaaac tcgatacagg gagatgatct
     3061 catgcacgac atacagcgca aagcgcaggc catgcaggaa cggatcgtcc agtggcggcg
     3121 ggcccttcac gccatgccgg aggtcggctt cgacaccccc ggcacggagc gattcatcgt
     3181 cgaggagctt caaaagatgg gcgtcacgga gatccgccac ggagacggaa aaaagggcat
     3241 cgtggccctc gtacagggaa agaagccggg aggcgtgctc ggcatccgcg ccgattacga
     3301 cgcgctgcac atgaccgagg agaccggcct ccccttcgcg gcgaccaacg gcaacatgca
     3361 cgcctgcggc cacgacgcgc acgcggcgat cctgctgggg acggcgaacc tgctgctgga
     3421 ctgcgccgac gagctggcgg ggacggtgaa actgatcttc cagccctccg aggagaacgg
     3481 caagggcgcg ctcgcgatga tcaaggacgg cgtcctcgag gagccgaaaa tggacgccat
     3541 catcgggctg cacataggaa gcctgtggaa gggcttgaac tccggcgagg tcgggtacag
     3601 gtacggcgcg ctgatggccg cggccgactg gttcaagatc accttcggag ggaagggcgg
     3661 tcacggcgcc acgccgcatc tcacggtcga cccgctcgcc atggcgtgcc agaccgtcgg
     3721 gctgctccag cagatcgtca gccgcgagac cagcccgctg gactccgccg tcgtcaccgt
     3781 cgggaccatc cagggcggca ccatgccgaa catcatcgcc ccaagctgca ccatcgcggg
     3841 caccgtccgc tgcctctcgc ccgaggttcg caaccacctg gagacgcgca tccgtgaaat
     3901 ctgcaccgac acggcgcgcg ccctccgcgg cagcgtggcc atagagtaca cctacggccc
     3961 tcccccgctc ctcaacgaca gggcggtcac ggacgagctg aagctcgcga tggagcagat
     4021 cgtcggcgcg gagaaggtcc gcgaggtcga gcagcccacc atgggcggcg aagacatggc
     4081 cttcttccag gagcacgtcc ccggctcgtt cttcttcctt ccggcgagct tcgaggacga
     4141 gagccgcaac ttcccgcacc accacccgaa gttcgacctc aacgaggacg tcttctgggt
     4201 cggcccggcg gtgatggtgg gcttcgccct ctcccgcggc tagggaggcc gccatgccgc
     4261 ttcatcggga gacgccgccg cttgagtccg tgaaataata gttaaacaat gcacgacata
     4321 tcgtttaaat ctcattaagt aaaagatttc ctcgcaccct cttgacagat ggggaaaaag
     4381 gctgtatcgt gctcaccaaa ttttgaggaa gggag
//
LOCUS       DAOPKU010000217         4397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_21738_length_4397_cov_3.236988, whole genome shotgun sequence.
ACCESSION   DAOPKU010000217 DAOPKU010000000
VERSION     DAOPKU010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21738_length_4397_cov_3.236988"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK849_07715"
     CDS             complement(<1..516)
                     /locus_tag="PK849_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HPE66051.1"
                     /translation="MNRTFRSLMTLSLILLLVSGLPLFAQTPETKPDDDEFALLESAR
                     LMRASGNVQFNFQDVDMVKFVRFMAELLQENIVLAPNIKGAISVISPKPVPLREARQV
                     LLSVLEMNGYTLQPVGGFSKIIPLQAWASAESDVLSGRTAPGEGDQTVSQVVPLNYLT
                     AEFLLPAVLQAS"
     gene            complement(513..902)
                     /locus_tag="PK849_07720"
     CDS             complement(513..902)
                     /locus_tag="PK849_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPE66052.1"
                     /translation="MKKRSAFTLIEVMVAVAVLGLSAAGSLRLLTMSVRALEDVRYER
                     RNLSLARTLWLAAVSDSLDERGREKEYSWETKRFTFERQEEVPEGFYCRQVDLVQGDP
                     MSRPSGSNANAFIFYVPDMKEKKERTK"
     gene            complement(917..1861)
                     /gene="gspC"
                     /locus_tag="PK849_07725"
     CDS             complement(917..1861)
                     /gene="gspC"
                     /locus_tag="PK849_07725"
                     /inference="COORDINATES: protein motif:HMM:NF041616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HPE66053.1"
                     /translation="MNLHSRSSSTRRISRLLSRQRLERLVALFFRVALVALFFGMGGY
                     WAAAALGEFLSAEEMQSTRSLLSVPLSRSLLPEASERPLTLKEFVGGDPFLVYVPPKP
                     KDESRQITAKELFDLEGIRLTGTLPGISVWIQEAKKPQAVVLKGQKIKGYDLTAVEED
                     RIILQKGPTTLTVLLRYSAETAASAKQSAPAVTAPRAAPQQTLSAARPGQQGSISRET
                     VNQLLMDPLAEMKKFRLRPKFDGDTAAGIEVQWLDDQSILTSLGVKAGDVVQTVNGVP
                     IRNMGDVVNVINSLMSGNTFDVQVVREGKPEMLNYNIK"
     gene            complement(1858..2472)
                     /locus_tag="PK849_07730"
     CDS             complement(1858..2472)
                     /locus_tag="PK849_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66054.1"
                     /translation="MKRFASVFGVLLCAFISFVAALYLFFPMDTALELLWRKGMLAAA
                     EKGVTLETAFVGVDEYFPLRIVFRNVRVAAPVASAEAGMLTVRPLFVESILSMSPTAE
                     IRLENFSLGIPLPGQPPIAFSTFYSKSSVRASAIQLSGIQTTGDLVIVGDLTVNPATA
                     KLDEADLHISGERASLLEFAKTMLPLQKDASGAWTLKRKGGENK"
     gene            complement(2469..3014)
                     /gene="gspM"
                     /locus_tag="PK849_07735"
     CDS             complement(2469..3014)
                     /gene="gspM"
                     /locus_tag="PK849_07735"
                     /inference="COORDINATES: protein motif:HMM:NF041618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HPE66055.1"
                     /translation="MKLPSFVSNPFDLLGGLDEASWKKKTFYVFLFALLAWGAAFSFW
                     SDGEELRARRTLQRSRFNELVAVLREYSALKSIGGGAKGGEAALAPVAGGDLLSVVSD
                     VVGSLGLRTNMISLSTASVRGGRNGVSISLEGLSAENLAMFLQEMDRRGIETFSADLR
                     AVRENEKQRTLTVFLLLGGAS"
     gene            complement(3011..4201)
                     /gene="gspL"
                     /locus_tag="PK849_07740"
     CDS             complement(3011..4201)
                     /gene="gspL"
                     /locus_tag="PK849_07740"
                     /inference="COORDINATES: protein motif:HMM:NF041617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HPE66056.1"
                     /translation="MQISTYFIKRPEGWSPLASSIEPIGGKRVESVPSGGALLLAPFK
                     TASIHSFSFPFRSANDVRNALALKFRPLLSGEDEVEIAPLFGGRTKGGSEGVALCIWE
                     GEVLDNEAGVSLHNNVVWPLPVALASAVKGDGGAVFRNDFVCASAFFRNGLPVYLRCR
                     SSSSSDGGIDGEVQRLVDYVSALGSDIASESVWVSTKEQELLEVARETVKQYPKLLEL
                     NISKPALAASLARERTARMLLKACGWAAAAGILFSLIQYTMLSQLRSSVETFTNEGTS
                     LYREIFGQNERVVDPLSQARAKLAELRGQGRTENSLSRTLSHLGRTWLDGNKKKAGYP
                     VLEQLRYSADGADITGTAEKMEAIQALRLAADSGGYKASLGDIQQIPGGGLRFTLSLR
                     RDAQ"
     gene            complement(4211..>4397)
                     /locus_tag="PK849_07745"
     CDS             complement(4211..>4397)
                     /locus_tag="PK849_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66057.1"
                     /translation="LARIGGFPPDAVPKLMNIATFKSDWFRVSFEVLFGDGEMIVTRG
                     ILKKTLKSWNTVQWEEP"
BASE COUNT         1119 a         1248 c         1077 g          953 t
ORIGIN      
        1 cgaagcctgc agcactgcgg gcaaaaggaa ctccgcagtc agatagttga gcggcactac
       61 ctgagaaaca gtctggtccc cctcgccggg agcagtgcgc ccgctcagga catcgctctc
      121 tgcggaagcc cacgcctgca atggaataat cttgctgaag cccccgaccg gctggagcgt
      181 atagccgttc atctcgagta ccgacagcaa cacttgacgc gcctctctca agggaaccgg
      241 tttgggagaa ataacggaaa ttgcgccttt aatattcggc gcaagcacaa tgttctcttg
      301 aagcagctcc gccataaaac gcacaaactt caccatatca acatcctgga agttgaactg
      361 tacgttcccc gaagcgcgca tcaatcgggc ggattcaagc agagcaaact catcgtcatc
      421 cggcttcgtt tcgggagttt gcgcgaagag cggcaacccc gaaacaagaa gcaaaataag
      481 gctcaatgtc atcagcgatc ggaaggtgcg gttcattttg ttctctcctt tttttccttc
      541 atgtcaggca catagaagat gaaggcattt gcgttgctcc ccgaaggacg cgacatcgga
      601 tctccctgaa caaggtcgac ttggcggcag taaaaacctt cagggacctc ctcttggcgt
      661 tcaaaagtaa agcgttttgt ctcccatgaa tactccttct ctcgcccgcg ctcatccaaa
      721 gaatctgaaa cggcagccaa ccagagagtc cgagccagag aaaggttgcg acgctcgtaa
      781 cggacgtctt ccagagcacg gacggacatg gtcagcaggc gaaggcttcc cgcggcggag
      841 agaccaagaa cagcgacggc aaccataact tcgatgagag tgaaggcgct cctttttttc
      901 atcccacacc cgctcctcat tttatattat agttcaacat ttccggcttc ccttcccgaa
      961 caacctggac atcgaaggta ttaccgctca taagggaatt gatcacgttg acgacatctc
     1021 ccatgttcct gatcggaacg ccgttcactg tctgaaccac atctcccgcc ttcactccga
     1081 gcgacgtaag gatgctttga tcgtccagcc actgtacctc tatgcctgcc gccgtatcac
     1141 cgtcgaattt aggtcgtaag cggaattttt tcatctccgc cagcggatcc atcagcagct
     1201 ggttcacagt ttccctggag atagagcctt gttgtccggg gcgtgctgcg gaaagcgttt
     1261 gctgcggcgc agctcgcggc gccgtaaccg caggtgcgga ttgtttcgct gacgccgcag
     1321 tttcggcgct ataacgcaaa aggacggtca aggtcgtcgg gcctttctgg agaattatgc
     1381 gatcctcctc gacggcggtc aaatcgtacc ctttgatttt ttgtcccttg aggacgacgg
     1441 cctgaggctt cttcgcctcc tgtatccaga cagaaatacc cgggagtgta ccggtaagac
     1501 gaataccctc aagatcaaag agctcttttg cggttatctg acggctttca tctttgggtt
     1561 tgggcggcac atacacaaga aaaggatctc ccccgacgaa ctccttcaat gtcaatgggc
     1621 gctctgaagc ttccggaagt aacgaacggg agagaggaac gctgagaagc gaccgggtac
     1681 tctgcatctc ctcagcggat aaaaactctc ccagcgccgc tgcagcccaa tatccgccca
     1741 tcccgaagaa gagcgccaca agtgcgacac gaaaaaaaag cgctacgaga cgttcgagtc
     1801 gttgtcggga tagaagacga gaaatacgcc gtgtagatga agagcgggaa tggagattca
     1861 cttattttcg cctccttttc ttttaagagt ccatgcgccc gacgcgtctt tctgcagcgg
     1921 gagcattgtc ttcgcgaatt caagcagaga agctctttct ccggaaatat gaaggtccgc
     1981 ttcatccagt tttgcggtgg ccggattcac tgtcaaatcg ccaacaatca cgagatcccc
     2041 cgtagtttga attcctgaaa gctgtatcgc ggaagcgcga acgctgctct tcgagtaaaa
     2101 cgtggagaac gctatcggag gctgtcccgg aagaggtata cccagcgaaa aattctccag
     2161 gcgaatttct gcagtcggcg acattgacag gatgctctct acgaaaagcg gccgtacggt
     2221 aagcataccc gcctccgcag acgctacagg agcagcaaca cgcacgttgc ggaaaacgat
     2281 gcgcagcggg aaatattcgt cgactcccac gaacgctgtc tccagtgtca cacccttttc
     2341 agcagcggca agcatccctt ttcgccaaag aagttccagt gccgtatcca ttggaaagaa
     2401 aaggtacaag gctgcaacaa aagaaatgaa cgcgcataaa agcactccaa aaaccgacgc
     2461 aaaacgtttc atgacgcgcc tcccagcaga agaaaaactg taagcgtccg ttgtttttca
     2521 ttttcccgca cggctcgaag gtcagcggag aaagtttcta ttccgcgccg gtccatttcc
     2581 tgaagaaaca tggcgagatt ttcagcggaa agtccttcaa gagaaatgga aacaccatta
     2641 cgtcctcctc gaaccgaggc agtgctcaag ctgatcatgt ttgtacgcag tccaagcgaa
     2701 ccaactacat ccgacaccac ggaaagaaga tctcccccgg cgactggcgc aagagcggct
     2761 tctccgcctt ttgcaccgcc cccgatagat tttaaagcag agtattcgcg taatactgcg
     2821 accagttcat taaaacggga acgttgcagc gtacggcgag cacgaagctc ttctccgtcg
     2881 ctccagaaag agaacgcagc tccccacgct aagagagcaa aaagaaaaac gtaaaaagtc
     2941 tttttcttcc aggatgcttc gtcaagtcct ccgagcaaat cgaaaggatt cgaaacaaac
     3001 gaggggagtt tcattgagca tccctcctca atgagagtgt gaatcgaagt cctccgcccg
     3061 gtatctgctg aatatcgccc aaggaagcct tatagcctcc actgtcggca gcgagccgaa
     3121 gcgcttgaat cgcctccatc ttctccgccg tacctgtgat atccgcacca tccgcagagt
     3181 agcgaagctg ttcaagcacc gggtacccag ctttcttttt attcccgtca agccaggtcc
     3241 ttcctagatg ggagagcgta cgcgacaatg aattctctgt tcttccctgg ccccgaagtt
     3301 ccgcgagctt tgctctggct tgagaaagcg ggtcgacaac tcgttcattc tgcccgaata
     3361 tttcacggta gagcgaagtc ccctcgttcg tgaatgtctc caccgaagag cgaagctggg
     3421 aaagcatcgt atactgaatg aggctgaaga gtatccctgc cgccgccgcc cagccgcatg
     3481 ccttcaaaag catacgggca gtccgttcac gggcaagcga cgcggcaaga gcaggtttcg
     3541 aaatgttgag ttccaggagt tttggatact gtttcacggt ctctcgcgca acctctaaca
     3601 gctcctgctc tttcgtcgaa acccataccg attcggaagc aatatcgctt ccgagcgcag
     3661 agacgtagtc cacaagacgc tgtacttctc cgtcaatccc accgtcactt gaacttgagg
     3721 atcggcaacg tagatagacc ggcagcccat ttcgaaagaa cgcggacgca cagacaaaat
     3781 catttctgaa taccgccccg ccgtctcctt tcaccgcaga ggcgagagcc accgggagag
     3841 gccatacgac gttgttatgg agcgacacgc ctgcttcatt atcaaggacc tccccctccc
     3901 aaatacacag agcaacccct tccgacccgc ccttggtcct tcctccgaag agaggagcaa
     3961 tctccacctc gtcctctcct gaaagaagag ggcgaaattt gagagcaagg gcatttcgca
     4021 catcgtttgc agatcgaaag gggaaggaaa acgaatgaat cgatgcagtt ttgaagggag
     4081 cgagaagcag ggctcctcca gacggtaccg attctactct ttttccgcca ataggctcaa
     4141 ttgaggacgc aagaggactc cagccctccg ggcgctttat aaaatatgtg gaaatctgca
     4201 ttggtcctat ctacggctcc tcccattgga ctgtattcca tgactttaag gttttcttca
     4261 gtataccccg agtgacaatc atctcaccat cgccaaataa aacttcaaag gaaacacgaa
     4321 accaatcgct tttgaacgta gcgatgttca tcaacttcgg aactgcatcg ggagggaacc
     4381 ctccaattct cgcgagg
//
LOCUS       DAOPKU010000218         4394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_21759_length_4394_cov_4.284628, whole genome shotgun sequence.
ACCESSION   DAOPKU010000218 DAOPKU010000000
VERSION     DAOPKU010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21759_length_4394_cov_4.284628"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..1235)
                     /locus_tag="PK849_07750"
     CDS             complement(321..1235)
                     /locus_tag="PK849_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPE66058.1"
                     /translation="MNFSLPERIEIMEVCPRDGFQSVKEFIPTEGKIAILDALAGTGI
                     STMEITSFVSPKAIPQMADAADVTAHFNTKWRGKITSVALVPNLRGAESALAAGVDWM
                     NFVISASVDHNMANTRRTVEESIEELKKVCALKGETKLRLSVATSFACPFAGDVPSEK
                     VVRVVDACLEAGIDGVTLCDTIGTTDPKYLANTLTEMRTRYGDYPFALHLHDTHGMAL
                     ANMLTALRMGFSSFDAAAGGLGGCPFAPGAAGNAATEDMANMAERMGIATGIDIPKLL
                     AVVRSMKADYGLTTYSHLAAAEKSCCSC"
     gene            complement(1240..2442)
                     /locus_tag="PK849_07755"
     CDS             complement(1240..2442)
                     /locus_tag="PK849_07755"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPE66059.1"
                     /translation="MANARKGALEGLRVLDLTRVLAGPFCTMMFADMGAEVIKIEEAG
                     KGDDTRQMGPFKNGESAYFMNLNRNKKGITLNLKNPKGKAIFLDLVKKADIVIENFRP
                     GTMEKLGLGYEELKKLNPAIIYAAISGFGHTGRYSQRPGYDIISQAMSGLMSTTGWPD
                     GEPTRTGTAMGDVLGGLSCAIGILSAVFNRVSTGLGQKVDVALVDSAVASLEIINMIY
                     LVEGRIPQRIGNRYESTYPYDSFRAADGSLVIGVANDKLWAQLCEAMDLQHLINDIRF
                     ARVRDRVANHAELKPLIEEWTTRFTVDEVCEKVSAAGVPCAPILNIEQVVNDPHIGGD
                     REMFVEMEHPVAGKLKVTGSHIKLSDTPAGVRTPSPALGQNNEEILGSLLGMTSEAIA
                     ALRAEGAV"
     gene            complement(2490..3185)
                     /locus_tag="PK849_07760"
     CDS             complement(2490..3185)
                     /locus_tag="PK849_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE66060.1"
                     /translation="MLKTHSFRHSTLGEEVASYLGKELLLSENYTPGSFIREEELAKA
                     LNVSRAPVREGIKILEGFGLLRTIPQKGAMVVDFSPEEVEELYDIRYALEEIVFREII
                     EKGLFTRDDYDIFRKKLDRMLLLGMNVDSKETILLEFSRIDMDFHTSLAEKSGRRWTL
                     RMLEMVYRQIQQAVLKDLAAENDMKALVDEHIRILDALLAGDLEELKKGRFFSYFQRR
                     IAPGREDRLASGV"
     gene            complement(3338..3925)
                     /locus_tag="PK849_07765"
     CDS             complement(3338..3925)
                     /locus_tag="PK849_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPE66061.1"
                     /translation="MEAVSRCNTRRLVWTALLSALAAVLMYIDMALPIFPRFLKIDIS
                     DLPALVASFSYGPVAGVFTELIKNLIHMFSTSSAGIGEAANFLMGSALVVPAGLIYRT
                     NRTKKGAVAGLVAGTLCMTVTASFANYFILLPFYSHLMPLEKIISMSSAVIPAIHDKF
                     TLILYAVIPFNLLKGFLVSIVTIRLYKRISAMLCR"
     regulatory      complement(3997..4152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT          887 a         1235 c         1271 g         1001 t
ORIGIN      
        1 aaaataccgg aacctgcgcc ggtagagaaa gagtatcacg aaaaggcgta catttccacg
       61 tccgctttga aaccggaaag aggaagaaga cgcccccgtg gaacggcagc cgggttttgt
      121 agcaactccc cgagttacgc tccctccgga acgagtgtga agaaaatgat ggggcacgaa
      181 atcgaaagcc actcgttgag agattcacga tgtgttcatg agactctggc tttcttgtgt
      241 ggtacagcgt actccataac aaggcccccc ttcggcaagc aactctgcag aaggggggcc
      301 ttgttctttg ccgaatcgta tcagcagcta cagcaggatt tttccgccgc cgcgaggtgg
      361 ctgtacgtcg tcaacccgta gtcggccttc atgctgcgta cgacggcgag cagctttgga
      421 atatcgatgc ctgttgcaat tcccatgcgt tccgccatat tcgccatgtc ctccgtggcg
      481 gcgtttccgg ctgcgcccgg cgcgaagggg cagcctccga ggccgcccgc cgccgcatcg
      541 aatgaggaga atcccatccg gagcgcggtc agcatgttcg caagcgccat tccgtgcgta
      601 tcgtgaagat ggagggcgaa cgggtagtct ccgtatctgg tccgcatctc cgtaagcgtg
      661 ttcgcaagat atttcggatc cgtggtgccg atcgtgtcgc agagcgtcac gccgtcgatc
      721 cccgcttcaa ggcaagcgtc cacgacgcgg acgacttttt ctgaggggac gtctccggcg
      781 aaggggcagg cgaaggacgt cgcaaccgag aggcggagct tcgtttctcc cttcagcgcg
      841 cagactttct tcagctcttc gatggattct tcaaccgttc ttcgagtgtt cgccatgtta
      901 tgatcgacgc tcgccgagat aacgaagttc atccagtcga cgcccgccgc gagggccgac
      961 tcggcgcccc gaagattcgg tacgagggcg acggaggtga tcttcccccg ccactttgtg
     1021 ttgaagtgcg ccgtcacgtc cgcggcgtcc gccatctgcg gaatagcctt cggactcaca
     1081 aaagaggtaa tctccatcgt ggagatgccc gtcccggcga gcgcgtcgag aatcgcgatc
     1141 tttccctcgg tagggatgaa ctcctttacg ctctggaagc cgtccctggg gcagacctcc
     1201 ataatctcga tccgttcggg cagagagaag ttcatgaggc tacaccgctc cctctgctcg
     1261 aagcgccgcg atggcctcgg aggtcatgcc gagaagcgat cccaggatct cctcgttgtt
     1321 ctgtcccagg gcgggggagg gggtacgtac tccggcaggg gtatcggaaa gcttgatatg
     1381 gcttccggtg accttcagtt ttcctgcgac ggggtgctcc atctcgacga acatctctct
     1441 gtcgccgcct atatgcgggt cgttcacgac ctgttcgata ttcagaatcg gcgcgcacgg
     1501 cacacccgcc gcggagacct tctcgcagac ttcgtccacg gtgaagcggg tggtccactc
     1561 ctcaatcaac ggtttcagct cggcgtggtt cgccacccgg tcccgcaccc gcgcaaagcg
     1621 gatgtcgttg atcagatgct gcaggtccat ggcctcgcag agctgcgccc agagtttgtc
     1681 gttggccacg ccgatgacga ggctgccgtc ggcggcgcgg aaggagtcgt acggataggt
     1741 ggactcgtag cggttgccga tacgctgcgg tatccgcccc tcgacgaggt agatcatgtt
     1801 gatgatctcc aggcttgcca ccgccgagtc gaccagcgcc acgtcgacct tctgtccgag
     1861 ccctgtggag acgcggttga atactgccga aagaatgccg atcgcgcagg agagcccgcc
     1921 caatacgtcg cccatggctg ttcctgtgcg ggtcggttcg ccgtcgggcc agccggtggt
     1981 gctcatgagg ccgctcattg cctgggagat gatgtcgtac cccggccgct ggctgtagcg
     2041 cccagtgtgg ccgaagccgg aaatagcggc gtagatgatc gccgggttca attttttcag
     2101 ctcttcgtat cccaaaccga gcttttccat cgtgcccggg cggaagttct cgattacgat
     2161 atcggccttc ttcacaagat cgagaaagat cgcctttccc ttggggttct tcaggttcag
     2221 cgtgattccc ttcttattcc ggttcaggtt catgaagtac gcgctctcac cgttcttgaa
     2281 agggcccatc tgccgggtgt cgtcgccctt gccggcctct tcgatcttga tgacctctgc
     2341 gcccatgtcg gcgaacatca tcgtacagaa cggtcccgcg agcacgcggg taagatcgag
     2401 gactcgaagt ccttcaagcg ctccttttcg tgcatttgcc attcttattc ctccccttga
     2461 gtgaattcgt tctttcccgc gcaggtgttt catactccgg aagccagtcg gtcttctctt
     2521 ccgggggcga ttctcctctg aaaatagctg aaaaaacgtc cttttttcag ttcttccaga
     2581 tcgccggcca gcaacgcgtc cagaatgcgt atatgctcat ctaccaaagc tttcatatcg
     2641 ttctcggcgg caagatcctt caacaccgcc tgctgaattt gtctgtagac catttcgagc
     2701 attctcaacg tccatcgtct cccggatttc tccgcgaggg atgtgtggaa gtccatgtcg
     2761 attctgctga attcaagaag gatggtttcc tttgagtcga cattcattcc aaggagaagc
     2821 attctgtcga gttttttcct aaagatgtcg tagtcgtcgc gggtaaacag ccctttttcg
     2881 ataatttcac ggaagacgat ttcttcgaga gcgtaacgga tatcgtagag ttcttccacc
     2941 tcttcggggg agaaatcgac gaccatcgct cccttctgcg gaatagtgcg gagaaggccg
     3001 aatccttcca gtatcttgat tccttcgcgg accggcgccc tgctcacgtt caacgctttc
     3061 gcgagctctt cctcccttat gaaggagccg ggagtgtagt tttcggagag cagcagctct
     3121 ttgccgaggt aggaggccac ttcctctcca agcgtactgt gtctgaatga atgtgttttg
     3181 agcacgccga aacctccttc aggatgcgat atgttaaatg tcgacatttt agatattgac
     3241 atgattatac agactgcggt gaaggtgtca agaaaaaaca aacccgccgc aaggcgggtt
     3301 tgtcgcgtga tcgaggtgtt ctgtgttcag gagaatacta gcggcacagc atggcgctga
     3361 tgcgtttgta caggcggatc gtcacgatgg agaccagaaa tcccttcagg aggttgaacg
     3421 ggataacggc gtaaaggatg agggtgaact tgtcatggat cgcggggatc accgccgagg
     3481 acatcgaaat aatcttctcg aggggcatca gatgcgagta gaaggggagg aggatgaagt
     3541 agttcgcgaa ggatgctgtg accgtcatgc agagggttcc tgctaccagt ccggcgacgg
     3601 cgcctttttt cgttcggttt gtccggtaga tcagccctgc ggggacgacg agggccgagc
     3661 ccatcaggaa attcgccgcc tcgccgattc ccgcggagga tgttgagaac atgtggatga
     3721 ggttcttgat cagctctgtg aagactccgg caaccgggcc gtaggagaac gaggcgacca
     3781 gcgccggaag atcggagatg tcgatcttca gaaaacgggg aaatataggc agcgccatgt
     3841 cgatgtacat cagcaccgcg gcaagtgcgg agagcagcgc ggtccagacg agacggcgcg
     3901 tgttgcagcg ggatacggct tccatagtac gatcaagtcc tttcgaaaaa gaataatgcc
     3961 ccggacgcga cgcatccggg gcacgtgtgc agtcttcgac atcctcttcc atcctgactc
     4021 cggtccgtcc cgaaggacgc ccttacagtc ggctccggat tcgcaccgga tcggcctttc
     4081 ggctcgcggg cttggagccg tttccggccc gtcaccgccg gtggggagtt ccaccccgcc
     4141 ccgaagatgt tcaactctat tgtacaccgc tttttcgaaa atttctattg acatgggcgc
     4201 gcaatcttcc tataatcccg gaaaaccgcg aaagtgcagg ctcgttcctc cacggggcgg
     4261 gctttttttg attgcattca cgaaaggaga tgttcctatt gaaaatagca gtagtcggcg
     4321 ctaccggaga agttggacgc atgatgacca gggtgctcca agagcagaac gtgaagcccg
     4381 ggaagatcga tttt
//
LOCUS       DAOPKU010000219         4386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_21814_length_4386_cov_5.253983, whole genome shotgun sequence.
ACCESSION   DAOPKU010000219 DAOPKU010000000
VERSION     DAOPKU010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21814_length_4386_cov_5.253983"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK849_07770"
     CDS             <1..332
                     /locus_tag="PK849_07770"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HPE66062.1"
                     /translation="LVGMDRAAAQAAFSAFLNDRSLTPPQIRFVETIIDQLTAHGVME
                     PSALYEPPFTRIDSGGPDALFDGRENVVAGIFETLDAINSGLYDEGTSLRVARGKSGG
                     TMHCESV"
     gene            complement(374..1111)
                     /locus_tag="PK849_07775"
     CDS             complement(374..1111)
                     /locus_tag="PK849_07775"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPE66063.1"
                     /translation="MEKNLRPAGIIPSEKSAGWEAPEQRALLVEIFKIPLFSESFFLG
                     GGTCLSVMYFGHRQSQDLDLFTVDEISIPVAWGMLARRLPRRGYALETTISISEGFAS
                     GVFQKNGVMTKVDLVQDMFVEDLRREQVVLDGVPIRVDNFGNLVTGKFSAFLSRGEPK
                     DIIDLSRILNHAKDTGQFIPFLERLLTETSKRDALADDDTLLSEIFTKIERQHSDLFH
                     PEIARMVLAFLETLQRRKKALSEGEHS"
     gene            complement(1027..1410)
                     /locus_tag="PK849_07780"
     CDS             complement(1027..1410)
                     /locus_tag="PK849_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66064.1"
                     /translation="MEKVSPENFSFDIIRFFRYPKIFWDDKEIRNASPSELKRLIDRL
                     RSSPVPLDRLRFKAFVRRMIERGSASDALLFLTPDEIREFLEQESVYCVDERMESLWK
                     KISGLPESFQVKKVRAGKPRSSGLS"
     gene            1588..1725
                     /locus_tag="PK849_07785"
     CDS             1588..1725
                     /locus_tag="PK849_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66065.1"
                     /translation="MRLDADGPDALFDGRENVVANIFETLDAINSGLYDERTSLRVAR
                     K"
     gene            1906..2916
                     /locus_tag="PK849_07790"
     CDS             1906..2916
                     /locus_tag="PK849_07790"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HPE66066.1"
                     /translation="MPRRFSFILAALLTLVLIPYFPGISCAEPGYSFDPSLAPSSPDY
                     SLPKSWAVLPEEPLPYPVDIFFVHPTTYNGDENWNQSLEGESADPKVRGVMKSQAGVF
                     STSACLYAPYYRQANLAVLGTSEESPARRSLDVAYSDVEAAFDAYMTFWNKGRPFILA
                     GHSQGSEHLLHLLERRFGDAELRKKLVAAYIIGWSVTKDDLARYPFLKIAESPDQTGS
                     IVSFNTQGPNPGYSIVREGAVGVNPLTMTITGEVADADQNLGAVFFPDKGVMEIPHYT
                     GGQTVGGAFVIPTPSNINDLDAMKIPEFYHPYDYTFFYRNLQQNAELRTRTYLESHGI
                     KN"
     gene            complement(3038..3799)
                     /locus_tag="PK849_07795"
     CDS             complement(3038..3799)
                     /locus_tag="PK849_07795"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPE66067.1"
                     /translation="MNTIVEELIEYGLSHSKGERVEDVRVGLGYTAVKLDSAKAGVAC
                     MLRHRLGKTTCSLLPHAGSLAGSPADRIIPLLRSPNIVETSVGLATLNALIQQDRYDE
                     DSSNDDLVQLLNITSNDRVGMVGDITPIFNRIQKHAKECIVFDEGKSAWEGITDTSLE
                     KEMLPRCDVALLSATTLLNNTFESVLSMVAGAREICVIGPSAPLLPDVFKKRGVTLLS
                     GRRFLDADRLLRIVSEAGGTKCFGPVSLKVNIQLK"
     gene            4127..>4386
                     /locus_tag="PK849_07800"
     CDS             4127..>4386
                     /locus_tag="PK849_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPE66068.1"
                     /translation="MNAHYVKFISACLLFGSNGIVASHILMSSHEIVFLRTLIGSLFL
                     LAVFVLTGGKFSGLRNGRHLAFLILSGVAMGASWIFLFEAYQR"
BASE COUNT          982 a         1160 c         1205 g         1039 t
ORIGIN      
        1 ctctggtggg gatggaccgg gccgcggcgc aggccgcctt ctccgccttc ctcaacgacc
       61 gtagcctgac gccgccgcag atccgcttcg tcgagacgat catcgaccag cttacggcgc
      121 acggcgtgat ggagccgtcc gccctctacg agccgccgtt cacccgcatc gactctggcg
      181 gccccgacgc actcttcgac ggcagggaga acgtcgtcgc cggcatcttc gagacgctgg
      241 acgccatcaa ttccggcctt tacgacgagg gcacaagcct ccgcgtcgcg aggggaaaga
      301 gcgggggaac gatgcattgc gagagcgtat aatctgttaa tggcaggtgt ttgtggtttg
      361 aaaaagagag acttcaggaa tgctctccct ctgaaagagc cttttttctg cgctgtaaag
      421 tttctaaaaa agcaagaacc atacgggcga tttccgggtg aaacaggtcg gagtgctgtc
      481 gttctatctt ggtgaagatt tcgctgagaa gagtgtcgtc gtcggcaaga gcgtctcttt
      541 tcgatgtttc tgtcagcagg cgttccagga acgggataaa ctgaccggta tcctttgcat
      601 gattcagtat acgcgagaga tcgatgatat cctttggttc tcctctgctt agaaaggcgg
      661 agaatttccc agtgacaaga ttaccgaaat tgtccactcg tataggaact ccgtcgagaa
      721 cgacctgttc acggcgcaga tcctcgacaa acatgtcctg aacaaggtcc acttttgtca
      781 tcacgccatt tttttgaaag actccggacg cgaatccttc gctgattgaa atcgttgttt
      841 ccagggcgta gcctcttctg ggaagacgcc tggcgagcat cccccacgca acagggattg
      901 aaatctcatc aaccgtgaac aggtcgagat cctggctttg acgatggccg aaatacatta
      961 ctgagaggca ggtaccgcct ccgaggaaga agctttcact gaaaagcggt atcttgaata
     1021 tctcaactaa gagagcccgc tgctccgggg cttcccagcc cgcacttttt tcacttggaa
     1081 tgattccggc aggccggaga tttttttcca taggctctcc atcctttcat ccacgcagta
     1141 aacgctttcc tgctcaagaa actccctgat ctcgtccggt gtcagaaaga gcagcgcgtc
     1201 gctggcgctg cctcgctcga tcattctgcg gacgaacgcc ttgaagcgga gtcggtccaa
     1261 tggaaccgga gaagaacgca gtctgtcgat caggcgtttt agctccgatg gcgatgcgtt
     1321 ccggatctct ttgtcgtccc agaagatttt cgggtagcgg aaaaaacgta tgatatcaaa
     1381 cgaaaagttt tccggcgaaa ccttttccat cgtctcaacc tcctttccga tggtcgggaa
     1441 ctctatgatt cgagtatatc atgtcgaaga ctgttcaaaa acgttgccga ggggcggcat
     1501 ccaggttgtg cgatagactc ttgaatccgg cggtcttgct tctataatac agctatgttg
     1561 gagtggggcg aacgagattt ttcatcgttg cgacttgatg cagacggccc cgacgccctc
     1621 ttcgacggca gggagaacgt cgtcgcgaac atcttcgaga cgctcgacgc cattaattcc
     1681 ggcctgtatg acgagcgcac aagcctccgc gtcgcgagga aataattcag taaaaggggg
     1741 aggggcatta tgaacggctg gtctgtgaga ggaggcccga aaaggttccg gcttgccggg
     1801 cgccggtttt gacggaagaa agtacacaaa acattggaga agattcgatt tatgctcgat
     1861 cgctgcatct aaaactgtac atacaaatta aaggagtgaa tactgatgcc cagaaggttt
     1921 tcttttattc ttgccgcgtt gcttactctc gtcctgatac catattttcc aggaatttcc
     1981 tgtgcggaac cggggtattc tttcgacccg tctctggctc cttcctcccc ggactattcc
     2041 ttgccgaaaa gctgggccgt tctgcccgaa gagcctcttc cctacccggt ggatatcttt
     2101 tttgttcacc ctacgacgta taacggcgat gaaaattgga accagtccct tgagggcgag
     2161 agcgccgatc caaaggtacg aggggttatg aagagccagg cgggagtttt ttcaacatcg
     2221 gcttgtctct acgccccata ctaccgtcag gcgaatctgg ctgtgctggg cacatctgag
     2281 gagagcccgg ccagaaggtc ccttgacgtg gcctactcgg acgtggaggc tgccttcgac
     2341 gcctacatga ctttttggaa caaggggagg ccctttatcc tggcaggtca cagtcaagga
     2401 tcggaacacc tgctgcatct tttggaacgg cgtttcggcg atgctgaact gaggaagaag
     2461 ctggttgcgg cctatatcat cggctggtcg gtcacgaaag acgaccttgc ccgatatcct
     2521 tttttaaaga tagccgagag cccggaccag actgggagca tcgtcagctt caacacacaa
     2581 ggacccaatc ccgggtacag catcgttcgg gaaggggccg tgggcgtcaa tccgctcacc
     2641 atgaccatca ccggggaggt tgcggacgcg gatcagaact tgggggcggt attttttccc
     2701 gacaagggag ttatggagat tccccactat acaggagggc agaccgtcgg cggggctttc
     2761 gtcatcccga cgccctccaa catcaacgac cttgacgcca tgaaaattcc ggagttttac
     2821 cacccttacg actatacgtt cttctacagg aatcttcagc aaaacgccga acttcggaca
     2881 cgtacgtacc tggaatcgca tggcataaaa aactgacaat cctccggcgg cccgggagtt
     2941 gaactttttt tccccgttta ctcccgggcc gcttcccttt tgaaaaactt tgtattgtaa
     3001 ttttaagaag gcgcttcgcc agtggagcag ttttcttcta tttcagctgg atattgactt
     3061 tcaggctgac cgggccgaag cacttggttc ccccggcttc gctgacgatg cgaagcagcc
     3121 ggtcggcgtc gagaaaacgg cgtccggaaa gcagcgtcac gccccgtttt ttgaacacgt
     3181 cgggcagcag aggcgccgaa ggaccgatga cgcagatctc cctcgcgccg gccaccatcg
     3241 agagcacgct ttcgaacgtg ttgttgagga gcgtcgtcgc ggaaagaaga gcgacgtcgc
     3301 agcgtggaag catctccttt tcaagcgacg tgtcggtaat cccctcccag gcgcttttcc
     3361 cttcgtcgaa gacgatgcat tctttcgcgt gtttttgaat cctgttgaaa atgggcgtaa
     3421 tatcgccgac cattcccacc cggtcgttcg aggtgatgtt gagcagctgg acaagatcgt
     3481 cgttggaaga gtcttcatcg tacctgtcct gctggataag cgcgttgagg gtcgccagtc
     3541 cgacggatgt ctccacgatg ttcggagaac gcaggagagg aataatccgg tccgcggggc
     3601 ttccagcaag ggaaccggcg tgtggcagaa gggagcaggt tgtctttccg aggcgatgtc
     3661 ggagcatgca cgcaactccg gcctttgcgg agtcaagctt gaccgcggta tagcccagtc
     3721 cgacccggac gtcttccaca cgctctccct tggaatggga gagtccgtac tcgataagct
     3781 cttcgacgat tgtattcaca gctatattcc ctcctcgaca agatgaccgg gcgtcttaaa
     3841 aagcgtagaa tggtgtgcaa accggtaaag cggagaagca gcctttccgc gcattctgac
     3901 ccgaaaaatg gagctatgtg ttctgttgac atgatgccac aaaacagtca aacatgcaac
     3961 tgataatcat tatcatatta aagagtgggc cttgtgccgt gaaccgccga agatgcactg
     4021 aagaagttct ttgatggcgc cactcgataa ttcgagcata tgagagagaa tagcgctgta
     4081 tctaaggagt ctccgaactt tttgacttga agagaggtga agtgtaatga acgcacacta
     4141 cgtgaaattc atatcggcgt gcctgctgtt cggctcgaac gggatcgtag ccagccatat
     4201 tctgatgagc agtcatgaga tcgtctttct gcggacgctg atcggcagtc tgttccttct
     4261 cgccgtattt gtcctgaccg gcgggaagtt ctccgggttg cggaatggac gccacctcgc
     4321 gttcttgatt ctgtcggggg tcgccatggg cgcgagctgg atttttctgt tcgaggcgta
     4381 ccaacg
//
LOCUS       DAOPKU010000220         4373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_21907_length_4373_cov_4.576193, whole genome shotgun sequence.
ACCESSION   DAOPKU010000220 DAOPKU010000000
VERSION     DAOPKU010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21907_length_4373_cov_4.576193"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PK849_07805"
     CDS             <1..994
                     /locus_tag="PK849_07805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPE66069.1"
                     /translation="PDERVRGLMVSKDARCFSMTPLLYKEEMVKAFGDVPFYAEWNDH
                     EGFTGAFRRGCEHLGLVGAKIAFNDGVRAIDMLAIRDAMELQMCNGGKVLDPLRSRKD
                     EEELGYMREASRMVDEVVRKLHGFIRPGMTERQIAKQIPEFFEEAGSNGISFSPIVAS
                     GPNGSMPHYGGDQRVIEKNDVIILDLGGRHMSYCSDTTRTFFVGTPTEEQRKVYEIVR
                     QAQAAGEAAVQPGVTGQEVDRAARKVIVDAGYGDYFFNRVGHGIGLAVHESPYIIEGN
                     DKPLEPGNVFSVEPGIYLPGKFGIRIENIVAVRPDGTGEALNHFPRDIESVCIK"
     gene            complement(1076..2086)
                     /gene="dctP"
                     /locus_tag="PK849_07810"
     CDS             complement(1076..2086)
                     /gene="dctP"
                     /locus_tag="PK849_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE66070.1"
                     /translation="MRKVTRYFLLVPALVVLACLVRPAAAAPETVLRFAGQFPAEHTS
                     TKLMQQLADEVKEKTGGRIEIRLHPDNELGDYTLIFEELIRGSIDMAAITVPSQFDPR
                     LELCNINGFVRTFDEARTVFAPDGWVFKKMDELNSALGVKLLGFQFEGFIGIGSTKPV
                     NEPLNPEVKKGILLRIPNMEVFKLGAEAMGYRTITIPWDEVHGSLQNGFAEGVYGMTP
                     AAAYTILKDVIKYWYDLRHSVENLNYMMSLKTWEKLSEEDRGIIQNACAKISAVSVYL
                     AEKDQEKYLQSMRDAGIEVHTYTPEELAPLFVKVSATWDKLAERFSQELIDEFKKEFA
                     RQ"
     gene            2506..3387
                     /locus_tag="PK849_07815"
     CDS             2506..3387
                     /locus_tag="PK849_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HPE66071.1"
                     /translation="MKHQPSPSILQRILQFQRDELTSSILYEYIAGRQKDEGNRTALL
                     EIAKAERSHYTTWKGYTGQELRPNMLKIFFYKIIHMLLGDTFTIKLFEIGETFGAQEL
                     REIEGEIPEARTIMKEEEEHEDRLMEMVDEERLHYIGSMVLGLNDALVELTGTIAGLT
                     FALMNTRLVALSGIITGVSATLSMAASNYLAERADGNAKALKSSFYTGMAYLVTVALM
                     VLPYLLLPNSLYLAAFAILLAVVVMIIAVFNYYVSVVQSIPFWPRFAEMGTISLSVAA
                     ISFVIGLLAKHLLGIEI"
     gene            3456..>4373
                     /locus_tag="PK849_07820"
     CDS             3456..>4373
                     /locus_tag="PK849_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPE66072.1"
                     /translation="MVNRYARRRCVQGQNCFREGDAGARRVLRGLSGANVARNPALPP
                     VARDRRALLASGGNVRPCDGRRKHGHHVHSEREDAGLSAADSAGDPGGFEDAGDLFIP
                     TKALASDQFRALSPLLEHFGAGRVQAGIYDGDTPPNERSRIRGSANIILTNPEMLNAT
                     FLPNHGSFGFNFLFANLKYVVVDELHSYRGAFGSHLANLMKRLARICRYYGSSPRFLC
                     SSATIANPVELAEKICGAPFSLVSKDGSPAPEKRYYLWQPPLVKGGAFRISPAREATG
                     LIAQLVLRKSSFLAFCKSRNAVEVVLKESR"
BASE COUNT          931 a         1335 c         1282 g          825 t
ORIGIN      
        1 ccccgatgaa cgggtcaggg gactgatggt ctcgaaggac gcccgctgct tctcgatgac
       61 ccccctgctc tacaaggagg agatggtgaa ggcgttcggc gacgttccct tttacgccga
      121 gtggaacgac cacgaaggct tcaccggcgc gttccggcgg gggtgcgaac acctcgggct
      181 cgtcggcgca aagatcgcgt tcaacgacgg cgtgcgggcg atcgatatgc tcgcgatcag
      241 agacgcgatg gagctgcaga tgtgcaacgg cggcaaggtg ctcgacccgc tccgctcccg
      301 caaggacgag gaggagcttg ggtacatgag ggaggcgtcc cgcatggtcg acgaggttgt
      361 gcggaagctg cacggcttta tacgcccggg aatgaccgaa cgacagatcg ccaaacaaat
      421 ccccgagttc ttcgaggagg cgggctccaa cggcatctcc ttcagcccta tcgtggccag
      481 cggccccaac ggctccatgc cgcactacgg cggagaccag cgggtgatcg agaagaacga
      541 cgtaatcatc ctcgacctcg gcggccgcca tatgagctac tgttcggaca cgacgcgaac
      601 gttcttcgtc ggcacgccga ccgaggagca gaggaaagtg tatgagatcg tccggcaggc
      661 ccaggccgcc ggagaagcgg cggtacagcc cggagtcacc ggccaggagg tcgacagggc
      721 ggcgcgcaag gtcatcgtcg acgcggggta cggggactac ttcttcaacc gcgtggggca
      781 cgggatcggc ctcgcggtgc acgaatcccc ctacatcatc gaggggaacg acaagccgct
      841 cgagccgggg aacgtcttca gcgtcgagcc gggcatctac ctgccgggga agttcggcat
      901 caggatcgag aacatcgtcg ccgtgcgccc cgacggaacg ggcgaagcgc tcaaccactt
      961 cccaagagac atcgagtcag tctgcatcaa atagtcgcag aaccaacgga gaacaggaaa
     1021 aaggggcgcc cggaaagagg acgccccttt tcatatcctg gcggaagccc gaacgctact
     1081 gccgggcgaa ctccttcttg aactcgtcga tcagctcctg cgagaagcgc tcggcgagct
     1141 tgtcccacgt cgcggacacc ttgacgaaca gcggcgccag ctcctcgggc gtatacgtgt
     1201 gcacttctat accggcgtcg cgcatactct gaaggtattt ctcctgatcc ttctcggcga
     1261 ggtagacgct gactgcggag attttcgcgc aggcgttctg gatgattccg cgatcctcct
     1321 cggagagctt ctcccaggtc ttcaggctca tcatgtagtt cagattctcc accgagtggc
     1381 gcaggtcgta ccagtacttg atcacgtcct taaggatggt gtacgccgcc gcgggcgtca
     1441 ttccgtacac tccctcggca aagccgttct ggagcgagcc gtgcacctcg tcccacggga
     1501 tggtgatcgt ccggtagccc atcgcctcgg cgccgagctt gaagacctcc atgttcggaa
     1561 tgcgcagcag gattcctttt ttgacctcgg gattcaacgg ctcgttcacg ggcttggtgc
     1621 ttccgatgcc gatgaaccct tcgaactgga agccgaggag tttcacgccg agcgcggagt
     1681 tcaactcgtc catcttcttg aatacccatc cgtcgggagc gaagaccgtt ctggcctcgt
     1741 cgaacgtccg gacaaagccg ttgatgttgc agagctccag ccgcggatcg aactgactgg
     1801 gaacggtgat cgccgccata tcgatggaac cgcggatcag ctcctcaaaa atgagcgtat
     1861 agtcgccaag ttcgttgtcg gggtggagcc tgatctctat tcgtccgccg gtcttctcct
     1921 tcacctcgtc ggccagctgc tgcatcagct tcgtcgacgt gtgctccgca ggaaactgtc
     1981 ccgcgaaacg gagcaccgtc tccggggcgg ccgcggcggg gcggacaagg catgcaagaa
     2041 caacaagcgc gggaacaagc aagaaatacc gagtaacctt tcgcattcaa cgcactcctc
     2101 tcggaataaa acgatctgtt ccggttgcag tcaaccggcc cggcgtcctt ggagcattca
     2161 agctccggcg ccattcccac ccttctccgg actgagacag accgcgttca ccccctcaag
     2221 agttccgtat cggacgtttt cccgtctctc cggatgtttc aagaaaaccc ggctgcatat
     2281 tttcaggaga cttcatttca cttcgcaaga cgatattttc ctcattgtag cagcgtgtac
     2341 aaccgaagtc cacacccgtc gaacaaaaat gttcccaaat tcacaacgtc gacagccgag
     2401 cgccaaagac gtttcgacat aaatttcaca tggtataata aaaagttaca tactatcgca
     2461 aaatcctcaa gctctctgca gaaaatgtga aaggcaggca aaaatatgaa acatcaaccg
     2521 agtccatcca tacttcaacg gatcctacag ttccagcgcg acgaactgac ctccagcatc
     2581 ctgtacgagt atatcgccgg acgtcagaag gacgagggta acaggaccgc cctgctggaa
     2641 atagcgaagg cggaacggag tcattacaca acgtggaaag ggtacacggg acaggaactc
     2701 cgcccgaaca tgctcaaaat tttcttctat aaaattatac acatgctcct gggcgacacc
     2761 ttcaccatca agctttttga aattggagag accttcgggg cgcaggagct gcgcgaaatc
     2821 gaaggggaaa ttccagaagc gcggaccatt atgaaggaag aggaggagca cgaggaccgc
     2881 ctcatggaga tggtggacga agaacggctg cactacatcg gctccatggt gctggggttg
     2941 aacgacgcgc tcgtggagct cacgggcacg atcgccggac tgaccttcgc gctgatgaac
     3001 acccggctcg tcgctctttc gggaatcatc accggcgtct cggcgacgct ctcgatggcg
     3061 gcgtcgaact acctggccga acgcgccgac ggaaacgcga aggcgctcaa gtcgagcttc
     3121 tacaccggta tggcctatct ggtcaccgtc gcgctgatgg tgctccccta cctgctgctg
     3181 cccaacagtc tctacctggc ggcgttcgcc atcctgctgg ctgtggtggt gatgatcatc
     3241 gccgtgttca actactacgt ctcggtagtg cagtcaatcc ccttctggcc gcgcttcgcg
     3301 gagatgggaa ccatcagcct gagcgtcgcg gcgatctcct tcgtaatcgg cctgctcgcg
     3361 aaacacctgc tcgggataga gatataaaca aactcaacgt gtcacgctcc aagcgcaaaa
     3421 agttcagact accggaggct atcttcttat gaaacgtggt gaataggtat gcacgccgtc
     3481 gatgcgttca gggacaaaat tgttttcgag agggtgacgc cggagcgcgc cgcgtccttc
     3541 gcggactttc cggagcgaat gtcgcccgaa atccggcgct acctccggtc gcacgggatc
     3601 gacgcgctct actcgcatca ggcggaaatg ttcgaccgtg cgatggacgg cgaaaacacg
     3661 gtcaccacgt ccacagcgag cgggaagacg ctggcctttc tgctgccgat tctgcaggcg
     3721 atcctggagg attcgaggac gcgggcgatc tttttatacc caccaaggcg ctggcgagcg
     3781 accagttccg cgccctatcg cccctgctgg agcacttcgg cgcggggcgc gtccaggcgg
     3841 gaatctacga cggcgacacg ccgccgaacg agcgaagccg catccgcggc agcgcgaaca
     3901 tcatcctgac caacccggag atgctgaacg cgaccttcct gccgaaccac ggctcgttcg
     3961 gcttcaactt tctgttcgcg aacctgaagt acgtcgtggt ggacgaactg cactcctaca
     4021 ggggggcgtt cgggtcgcac ctcgccaacc tgatgaagcg cctcgccaga atatgccgct
     4081 actacggctc ctcgccgcgc tttctgtgca gctccgcgac gatcgcgaac cccgtggagc
     4141 ttgcggagaa gatctgcggc gcgcccttct cgctggtctc gaaggacggc tctcccgcgc
     4201 ccgagaagcg ctactacctg tggcagccgc cgctcgtcaa gggaggggct ttcaggatct
     4261 ctccggcacg cgaggcgacg gggctcatcg cgcagctcgt cctgcggaaa tccagcttcc
     4321 tggcgttctg caagtcgcgc aacgcggtgg aggtggtgct gaaggagtcc cgc
//
LOCUS       DAOPKU010000221         4370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_21937_length_4370_cov_4.585168, whole genome shotgun sequence.
ACCESSION   DAOPKU010000221 DAOPKU010000000
VERSION     DAOPKU010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21937_length_4370_cov_4.585168"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /gene="rpsQ"
                     /locus_tag="PK849_07825"
     CDS             <1..290
                     /gene="rpsQ"
                     /locus_tag="PK849_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPE66073.1"
                     /translation="RKPHRKVRTGTVVSDKMEKTVVIRVERFEKHPLYGKRIVRASKY
                     TAHDEKNECRMGDLVTIGETRPLSKTKRWTLLEIVRKAPVFDEVAENGGEA"
     gene            294..662
                     /gene="rplN"
                     /locus_tag="PK849_07830"
     CDS             294..662
                     /gene="rplN"
                     /locus_tag="PK849_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPE66074.1"
                     /translation="MIQLTTVLNVADNSGAKKLFCIQVLGGSKRKWGTIGDVIVASVR
                     EAIPNSYVAKGTVVKAVIVRTKKEIRRADGSYVRFDDNAAVIIDNNGDPKGTRIFGPV
                     GRELRAKKYMRILSLAPEVV"
     gene            679..1005
                     /gene="rplX"
                     /locus_tag="PK849_07835"
     CDS             679..1005
                     /gene="rplX"
                     /locus_tag="PK849_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPE66075.1"
                     /translation="MSKMRLKKGDRVRVISGKDAGKEGKVLRCIRDRDLLVIEGVNVV
                     TKHVKPSQKNPQSGIIKQEAPIYASKVMLVCPSCGAATRVGRGYLENGNKVRMCKACG
                     EIVDQV"
     gene            1020..1562
                     /gene="rplE"
                     /locus_tag="PK849_07840"
     CDS             1020..1562
                     /gene="rplE"
                     /locus_tag="PK849_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPE66076.1"
                     /translation="MAPRLLEKYKNDVSPKLAKEFSYKNVMEIPRLKKVVVNIGVGDA
                     KLDIKFMDNAIAELGAITGQRPLLKRAKKSVAGFKVRQGMPVACTVTLRGERMWEFLD
                     RLLSLALPSIKDFQGVSRRGFDGRGNYNLGLKEQLIFPEISYDKVIRSRGMNVSLVTS
                     AKTDEEAQALLRELGMPFTR"
     gene            1579..1764
                     /locus_tag="PK849_07845"
     CDS             1579..1764
                     /locus_tag="PK849_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPE66077.1"
                     /translation="MARKAIKHKAAQPPKFAVRKYNRCPLCGRVHAFMRRFNMCRCCF
                     RKLAREGKIPGVVKSSW"
     gene            1793..2197
                     /gene="rpsH"
                     /locus_tag="PK849_07850"
     CDS             1793..2197
                     /gene="rpsH"
                     /locus_tag="PK849_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPE66078.1"
                     /translation="MYVTDPIADMLTRVRNANMVYHESVDVPMSKIKMEIARILKEAG
                     YIKNFKVVNDPKKAYSSIRLFLSYGPNRERVIQGLRRISKPGRRVYVGHADLPKVMGG
                     LGIAVISTPKGLKTDTEAGTLGLGGEVLCYVW"
     gene            2214..2753
                     /gene="rplF"
                     /locus_tag="PK849_07855"
     CDS             2214..2753
                     /gene="rplF"
                     /locus_tag="PK849_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPE66079.1"
                     /translation="MSRIGRKPIPVPSGTTVAVEGGAVVVKGPKGRLETPMVPGIDVA
                     VEGGIIVVTRQNEQKQTKAFHGLIRALVANMITGVSGGFKKDLEIVGVGYRAQMQGKK
                     LILNLGYSNPVEFEAPEGIELALEGPTKLSVKGIDKQAVGQVSAIIRGFRPPEPYQGK
                     GIRYAGEHIIRKAGKTGAK"
     gene            2770..3135
                     /gene="rplR"
                     /locus_tag="PK849_07860"
     CDS             2770..3135
                     /gene="rplR"
                     /locus_tag="PK849_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPE66080.1"
                     /translation="MKTKTRSILREIRHARLRKTLSGTVEKPRMAVFHSLKHIYVQFI
                     DDERGHTLAAASTLDPALRGTLLGTCNIETAKIIGKIAAERALAKGITTVVFDRGGHM
                     FHGKVKALADAAREAGLKF"
     gene            3152..3664
                     /gene="rpsE"
                     /locus_tag="PK849_07865"
     CDS             3152..3664
                     /gene="rpsE"
                     /locus_tag="PK849_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPE66081.1"
                     /translation="MIKKKLDAKGMELTERVVAINRVSKVVKGGKRFKFSVLVVVGDG
                     DRYVGVGMGKAKEISEAVRKGIDKAGKNLLEIKKIGNTIPHPIIGGFGAAEVLLKPAA
                     PGTGVIAGGVVRAIMELGGVKDVVSKVIGRTSNPINVAWATMQAVEEMRTPDEILRLR
                     GKKTLDAVAE"
     gene            3677..3862
                     /gene="rpmD"
                     /locus_tag="PK849_07870"
     CDS             3677..3862
                     /gene="rpmD"
                     /locus_tag="PK849_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPE66082.1"
                     /translation="MAKLRITWKKSGIGKPEVQKRTVRALGLRKLNHTVEHNDTPQIR
                     GMVNKVSHLVECVVVED"
     gene            3875..4321
                     /gene="rplO"
                     /locus_tag="PK849_07875"
     CDS             3875..4321
                     /gene="rplO"
                     /locus_tag="PK849_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPE66083.1"
                     /translation="MSLHELRPAPGSKTKGKRLGQGLGSGTGKTAGKGNKGQKARSGG
                     GVRPGFEGGQMPMMRRIPKRGFNNARFAKVYQIVNVEDIASRFEAGTTVGFEELHAVR
                     LVQKDRLPIKILANGEIDKALTIRANAFSAGAKAKIEAAGGKVEVV"
BASE COUNT         1118 a         1145 c         1343 g          764 t
ORIGIN      
        1 agcggaaacc tcaccgcaag gtccggaccg gtacggtcgt cagcgacaag atggagaaga
       61 ccgtcgtaat acgggttgaa cggtttgaaa aacaccccct ctacgggaag agaatcgtca
      121 gggcgagcaa atataccgcg catgacgaaa agaatgaatg ccggatggga gatctcgtaa
      181 caataggcga gacccgtcct ctcagcaaga cgaagcgctg gacgcttctg gagatcgtac
      241 gcaaagcccc cgtcttcgac gaggttgcgg agaacggcgg ggaggcgtaa gtcatgattc
      301 agctgactac agtcctcaac gtcgccgaca actccggtgc gaagaagctt ttctgcatcc
      361 aggtactcgg cgggagcaag cgcaagtggg gcacaatagg cgacgtcatc gtcgcctcgg
      421 ttcgcgaagc gattccaaac agctacgtcg ccaagggaac tgtcgtcaag gcggtgatcg
      481 tgcgcacaaa gaaggaaata cgccgtgcgg acggctccta tgtccgcttc gacgacaacg
      541 ctgcggtgat catcgataac aacggcgacc ccaagggcac ccgaattttc gggcctgtgg
      601 gcagggagct gagggcgaaa aagtatatgc gtatcctctc cctcgcgccc gaagtcgtat
      661 gacggaggat aacggataat gagcaagatg agactgaaaa aaggcgaccg tgttcgggtg
      721 atctccggaa aggatgcagg caaagaaggc aaagtccttc gctgcatccg cgacagggac
      781 ctgctggtca tcgagggagt gaacgtggtg acaaagcacg tgaaaccctc ccagaagaat
      841 ccccagtcgg gcataatcaa gcaggaagcc ccgatatacg ccagcaaggt catgctcgta
      901 tgcccctcct gcggcgctgc aacacgcgtc ggtcgcggct atctcgagaa cgggaacaag
      961 gttcgtatgt gcaaggcctg cggcgagatc gtggaccagg tgtaaggagg taagcgtgaa
     1021 tggcccctcg gctgcttgaa aaatacaaaa atgacgtctc gccgaaactg gcgaaggagt
     1081 tcagttacaa aaacgtgatg gaaatacccc ggcttaaaaa agtcgtggtg aacatcggtg
     1141 tgggcgacgc aaagctcgac atcaaattca tggacaacgc gatcgctgag cttggcgcga
     1201 ttacaggaca gcgtccgctt ttgaagagag cgaagaaatc ggtcgccgga ttcaaggtcc
     1261 gccagggaat gcccgtagcg tgcaccgtca cgctgcgcgg agagcgtatg tgggagtttc
     1321 ttgatcgtct cctttcgctc gcgcttccca gcatcaagga ctttcagggc gtctcccgcc
     1381 gcggtttcga cggaagaggc aactacaatc tcggtctcaa ggagcagctt atcttccctg
     1441 agatcagtta cgacaaggtc atccgttccc gtggcatgaa cgtctcgctt gtaacctcgg
     1501 cgaagacgga tgaggaagcc caagctcttc tccgcgagct gggaatgccg ttcacccggt
     1561 aggaaggggg agtcgaaaat ggcgagaaag gcaatcaagc ataaagcggc gcaacccccg
     1621 aagttcgcgg tgcgaaagta taacagatgt ccgctctgcg gccgtgttca tgccttcatg
     1681 agaagattca acatgtgccg ctgctgtttc aggaagcttg ctcgcgaagg gaaaattccc
     1741 ggcgtcgtca agtcgagctg gtagtcgagt ggactgaagg gggtcactag gaatgtacgt
     1801 taccgatccg atagcggata tgctcactcg cgtcaggaac gcaaacatgg tgtatcatga
     1861 gagcgtcgac gttccgatga gcaagataaa gatggaaatt gcccgcatcc tgaaggaagc
     1921 cgggtatatt aagaatttca aggtagtcaa cgacccgaag aaggcctaca gctcaatcag
     1981 gttgttcctc tcctacggac cgaaccgcga gagggtcatc caggggctgc gccgtatcag
     2041 caaaccgggc agaagagtct acgttggaca cgccgacctt cccaaggtca tgggcggcct
     2101 cggcatcgcc gtcatctcca caccgaaggg cctcaaaacc gacacggaag ccggaacgct
     2161 cggcctcggc ggcgaagtcc tctgctacgt gtggtaagga agaggtggag tagatgtctc
     2221 gaatcggacg caagccaatt ccggtaccct ccggaacgac cgtcgccgtc gaaggcggcg
     2281 ccgtcgtggt gaaggggccg aaagggcggc tcgaaacgcc catggttccg gggatcgacg
     2341 tcgccgtcga aggcggtatt atcgtcgtca cccggcagaa cgaacaaaaa cagacgaaag
     2401 cgttccacgg cctgatccgt gcgcttgtcg ccaacatgat cacaggcgtc agcggcggtt
     2461 tcaagaagga tcttgaaatc gtcggcgtcg gctatcgtgc acagatgcag gggaagaagc
     2521 tcatcctcaa cctcggctac tcaaatcccg tggagttcga agctcccgaa ggcatcgagt
     2581 tagccctgga aggaccgacg aagctgtcgg tcaaggggat cgacaaacag gcggtagggc
     2641 aggtatccgc gatcattcgc ggtttccgtc cgcccgagcc gtatcagggt aagggaatcc
     2701 ggtacgccgg cgagcatatc attcgcaagg ccggaaagac cggagcgaag taattcgagg
     2761 tgaatggaca tgaaaactaa aaccagaagc atattgcgtg agattcggca tgcccggctt
     2821 cggaaaaccc tctccggtac ggtggaaaaa ccgagaatgg ctgtgtttca tagcttgaag
     2881 cacatctatg ttcagtttat cgacgatgag aggggacaca ctctcgcggc ggcgtccaca
     2941 ctcgatccgg cactccgggg aaccctcctc ggaacctgca acatcgagac ggcgaagatc
     3001 atcggaaaaa tagccgccga gcgcgccctc gcgaaaggca tcactacggt tgttttcgac
     3061 aggggcggcc atatgttcca cggaaaagtc aaggccctgg ccgatgcagc ccgtgaagcc
     3121 ggactgaagt tctaactggg aggcgcatgc gatgattaaa aagaagttgg atgccaaggg
     3181 gatggagctc actgaacgcg tagtagcaat aaaccgcgtg agcaaagtcg tcaaaggcgg
     3241 aaaaaggttc aagttcagcg tcctcgtcgt cgttggcgac ggagaccgct atgtaggcgt
     3301 cggcatgggg aaggccaagg aaatttcgga ggccgtccgc aaaggaatcg acaaggcggg
     3361 gaagaatctt cttgagataa agaagatagg aaacaccatt cctcatccca tcatcggagg
     3421 cttcggcgca gccgaggtac tcctcaaacc ggcggcgccc ggaacaggcg ttatcgcggg
     3481 cggagtggtc cgtgcaatca tggagctcgg cggcgtcaag gatgttgtga gcaaggtgat
     3541 cggtcgcacc tccaacccga tcaacgttgc atgggcgacc atgcaggctg tcgaagagat
     3601 gcggacgccc gacgaaatcc tgcggctcag gggtaaaaag accctcgacg ccgtagcgga
     3661 ataggaggga tacgcaatgg ccaaattgcg gattacctgg aaaaagagcg gcatcggcaa
     3721 gccggaagtg cagaagcgca ccgtccgcgc gcttgggctg agaaagctca accacacagt
     3781 agaacacaac gatacgcccc agattcgcgg catggtgaac aaggtcagcc atctggtcga
     3841 gtgcgtcgtc gttgaagact aggaggaacg cgccatgagt ttgcatgaac tgcgcccggc
     3901 tcccgggtcg aagacgaaag gcaagcgcct cggacaagga ctcggaagcg gcaccggcaa
     3961 aaccgctggc aagggaaata aaggccagaa agccagaagc ggcggcggcg ttcgccccgg
     4021 attcgagggc ggtcagatgc ccatgatgcg gcgcatcccg aagagagggt tcaacaacgc
     4081 ccgctttgca aaggtgtacc agatcgtgaa cgtggaggat attgcctccc gtttcgaagc
     4141 ggggacgacc gtaggattcg aagagctgca tgcagtgcgt cttgtccaga aggatcgcct
     4201 ccccattaag attttggcca acggagagat agataaagct ctcacgattc gcgccaacgc
     4261 cttcagcgca ggagccaagg cgaaaataga agctgcgggt gggaaggtcg aggtggtcta
     4321 agtgcttgac tccttcaggg atgcatttcg acttccggat ctcaaacgaa
//
LOCUS       DAOPKU010000222         4346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_22101_length_4346_cov_4.646702, whole genome shotgun sequence.
ACCESSION   DAOPKU010000222 DAOPKU010000000
VERSION     DAOPKU010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22101_length_4346_cov_4.646702"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1373
                     /locus_tag="PK849_07880"
     CDS             114..1373
                     /locus_tag="PK849_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HPE66084.1"
                     /translation="MIDLEKANQEALSRLLAAQPLIVGMGIAKETVPGMKDKMLLHAG
                     PPLTWETASGPIRGGVMAACMYEGWAKTPEEAEKLAASGAIAFDPCHHHHAVGPMAGI
                     MSPSMPVFVLENRTHGNKAYVTMNEGLGKVLRMGAFGPEVIEHLQWMEKTLYPVLKAA
                     IEHAVKKGEEPDVKNMIAQALHMGDELHNRNKAGTSLFLRTLAPHMVETCKDSAQLAE
                     VFRFIDKTDHFFLNIAMAAGKASLEAAAGIEGSSMLTVMARNGTEFGLQVSGLGDRWF
                     TCEAALPDVLLFPGFTREDTNRDIGDSAIMETYGVGGFALAAAPAIVQFIGGTPKDAA
                     NYTLEMYEITAGENSMFTIPALNFRGTPTGIDVLKVVETGITPVLDTGAAHKEPGKGQ
                     VGAGIVRMPMDAFVKAAEAFADKYLSE"
     gene            1414..2217
                     /locus_tag="PK849_07885"
     CDS             1414..2217
                     /locus_tag="PK849_07885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPE66085.1"
                     /translation="MSNSLELKNWSNIKVYDLTIPIGIQTPPWPTYEPLQVKYFKRLA
                     PNGANGQLLTHSNHVGTHLDGPIHFCGHGGDIASLPLKDFLVGPGVVVDIQDIAEDYG
                     IYTPEDLEARADIREGDILIINTGYHRYGWNEPEADEVRYMVKHPGPTREFATWALKK
                     KIKWIGVDCGSADHPMNTKIREWMPVQARQADAHLKKKYGKGLDDFFPEEDYQVMHIA
                     LFPSNLIHAECVAGDIDKLSGKRVTIGCFPWRFVGGESCISRIVAFAEE"
     gene            2314..3189
                     /locus_tag="PK849_07890"
     CDS             2314..3189
                     /locus_tag="PK849_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HPE66086.1"
                     /translation="MLAFEMERPRSLAAAVEILGKYGSKAMIKAGGTDVLVWMRKHAV
                     NPEMLVDLSEIPELGGISFYPHKGIKIGAMCTVNQVAEHEDARRYYGALTDACLSHSD
                     PLIRNKATVLGNVCASVPSGDMIPAFAAYEAVLHVYGPEGERDILFADFITGPRKNVL
                     VPGEIVVSATLPLPAGQSTGCYIKLGRRNALDLAQVGVACVAIDGEKGREYRLAYGAV
                     APRPVRAAEAEDILAGQTSPDAPLLEKAAKAAAAAVNPITDVRASREYRFSMVEELTK
                     RAVSLCAERFQGGNA"
     gene            3186..3686
                     /locus_tag="PK849_07895"
     CDS             3186..3686
                     /locus_tag="PK849_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPE66087.1"
                     /translation="MMKRDVHFILNGRQTTLSVEPRTTLLRALREYGVTSVKRGCEEG
                     ECGTCTVVVDDLAQKSCMMLALEAEGKSVLTVEGLVGPKGELHPIQQAFIEEGAIQCG
                     FCTPGMIMTTYAFLRKTPDPSEDEIRAALAGNLCRCTGYIPIFRAVQKAAKTMRELGH
                     LPGGAR"
     gene            3703..>4346
                     /locus_tag="PK849_07900"
     CDS             3703..>4346
                     /locus_tag="PK849_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE66088.1"
                     /translation="MKQTVTRNGVGAWTTRKYDLEKAAGTLAFTDDLRFGPELLHARA
                     FRSTKPHAEILSVDVSEAMKVKGVVKVITGKDFIHRFGLYLMDRTPLAIGKVRYVGEP
                     VALVIGETEEAAEEGMLKVAAKYKDLPPIFDPVKAATDNSVLVHPELETYDHVPFITP
                     FPGTNIANWFRIRRGDVDKAFAEAEHVVEEAVNCPQIAHGFLEPHCCICKEDPAT"
BASE COUNT          881 a         1361 c         1358 g          746 t
ORIGIN      
        1 ctcgacgtaa aaacgcagaa aacggaggcg gtcgtctggg actggaagcc tcccgcagcg
       61 agcggggatc tcctctctcg tttaaagaag ctcaagaagg ggggcaactg aacgtgatcg
      121 atctggagaa agcaaatcag gaggcgctct cgcgcctgct cgccgcccag ccgctgatag
      181 tcggcatggg gatagcgaaa gagaccgttc cggggatgaa ggacaagatg ctcctgcatg
      241 cggggcctcc cctgacgtgg gagaccgcgt ccggtcccat ccgcgggggc gtcatggcgg
      301 cctgcatgta cgaagggtgg gcgaagacgc ctgaagaggc ggagaagctc gccgcgagcg
      361 gcgccatcgc cttcgatccg tgccatcacc accacgcggt ggggccgatg gcggggatca
      421 tgtcgccgag catgcccgta ttcgtcctgg agaacaggac gcacggcaac aaggcatacg
      481 tcacgatgaa cgagggactc gggaaggtgc ttcgtatggg cgcctttggc ccggaggtca
      541 tcgaacatct gcagtggatg gagaagacgc tctaccctgt gctcaaggcg gcaatcgagc
      601 acgcggtgaa gaagggcgag gagccggacg tcaagaacat gatcgctcag gcgcttcaca
      661 tgggcgacga gctgcacaac cgcaacaagg cggggacgtc gctcttcctc cgcacgctcg
      721 cgccgcacat ggtggagacg tgcaaagaca gcgcacagct cgccgaggtc ttccgtttca
      781 tcgacaagac cgaccacttc ttcctgaaca tcgcgatggc ggcggggaag gcctctctcg
      841 aagcggccgc agggatcgaa ggttcgagca tgctcaccgt catggcgcgc aacggcacgg
      901 agttcggcct ccaggtctcc ggcctcggcg accgttggtt cacctgcgaa gcggccctgc
      961 cggatgtgct cctcttcccc gggttcaccc gcgaggacac aaaccgcgac atcggcgaca
     1021 gcgccatcat ggagacctac ggcgtaggcg gcttcgcgct cgccgcggcc ccggccatcg
     1081 tccagttcat cggcggcacg ccgaaggacg cggcgaacta cacgcttgaa atgtacgaga
     1141 ttaccgcggg agagaacagc atgttcacca tccccgcgtt gaatttccgc ggcaccccga
     1201 ccgggatcga cgtcctcaag gttgtggaga cgggaatcac cccggtgctc gatacaggtg
     1261 cggcgcacaa ggagcccggc aaggggcagg tcggcgcggg tatcgtccgc atgccgatgg
     1321 acgccttcgt gaaggccgcg gaggccttcg cggacaagta tttgagcgag taattcttat
     1381 ccaagtttac taaaacataa ggaggtactt actatgtcca acagtctcga actcaagaac
     1441 tggagcaata tcaaggtgta cgatctgacc attcccatcg gcatccagac gccgccatgg
     1501 cccacatacg agccgcttca ggtgaagtac ttcaagcgcc tcgctcccaa cggagcgaac
     1561 ggccagctgc tcacccactc caaccacgtg ggaacgcacc tcgacggccc gatccacttc
     1621 tgcggccacg gcggagatat cgccagcctt ccgctgaagg acttccttgt cggccccggc
     1681 gttgtggtcg acatccagga tatcgccgag gactacggca tctacactcc cgaagacctt
     1741 gaagcccgcg ccgacatccg cgagggcgat atcctcatca tcaacaccgg gtaccaccgg
     1801 tacggctgga acgagcccga ggccgacgaa gtccgctaca tggtgaagca ccccggaccc
     1861 accagagagt tcgccacctg ggcgctcaag aagaagatca agtggatcgg cgtcgactgc
     1921 ggttccgccg accatccgat gaacacgaag atccgcgagt ggatgccggt tcaggccaga
     1981 caggccgacg cgcacctgaa gaagaagtac ggcaaggggc tcgacgattt cttccccgaa
     2041 gaggactacc aggtgatgca catcgccctc ttccccagca atctgatcca cgccgagtgc
     2101 gttgcgggcg acatcgacaa gctcagcggc aaacgggtca ccatcggttg cttcccgtgg
     2161 agattcgtgg gcggcgagtc ctgcatctcg cgcatcgtcg cgttcgccga agaataactc
     2221 cgttacgacg ggcgtcctcc tcctcatggt tttcagaggg tgcggggacg ccctcttcac
     2281 atactgattt ctcaccgaag gggtgatatg tcaatgctgg catttgaaat ggaacgcccc
     2341 agatcgctgg ccgcggctgt cgaaattctg gggaaatacg gttccaaggc gatgatcaag
     2401 gcgggcggga cggacgtgct tgtgtggatg cgcaaacatg ccgtcaaccc ggagatgctc
     2461 gtggatctct cggagattcc cgaactcggc ggtatttcct tctatcctca caagggaatc
     2521 aagatcggcg cgatgtgcac tgtcaaccag gtcgccgagc acgaggacgc ccgaagatac
     2581 tacggcgccc tgacggacgc atgcctctcc cactcggacc cgttgatccg gaacaaggcg
     2641 acggtgctgg ggaacgtctg cgcctccgtt ccctccgggg atatgattcc tgcgttcgcc
     2701 gcctacgaag ccgtcctgca cgtctacggc cccgaaggcg agcgcgatat tcttttcgcc
     2761 gattttatca ccgggcccag aaagaacgtt ctggttcccg gagagatcgt cgtctccgcg
     2821 acgcttcctc tgccggcagg ccagtcgacg gggtgctaca tcaagctcgg acgaaggaac
     2881 gcgctcgacc tcgcgcaggt cggcgtggcc tgcgtcgcga tcgacggcga gaagggcaga
     2941 gagtaccggc tggcgtacgg cgccgtagct ccgcgccctg tgcgcgccgc cgaggccgag
     3001 gatattcttg ccggacagac gtcccccgat gcgccgcttc tggaaaaggc cgcaaaagcc
     3061 gcggccgccg cggtgaatcc tatcaccgac gtccgggcga gccgtgagta ccgcttctcc
     3121 atggtcgagg aactgacgaa acgggccgtc tctctttgcg cggagagatt ccagggaggg
     3181 aacgcatgat gaagcgcgat gttcatttca ttttgaacgg cagacagacg accctttcgg
     3241 tcgagccccg caccacgctg ctgcgggcgc ttcgggagta tggggttacc agcgtgaaga
     3301 gaggctgcga agagggagag tgcggcacgt gcaccgtggt ggttgacgac ctcgcccaga
     3361 agtcgtgcat gatgctcgcc ctggaggcgg agggaaagtc cgtccttacc gtcgaggggc
     3421 ttgtcggccc caagggggag cttcacccga ttcagcaggc gttcatcgag gagggggcga
     3481 ttcagtgcgg tttctgcacc cccgggatga tcatgacgac ctacgccttc ctccggaaga
     3541 cccccgatcc ctcggaggat gagatccgcg cggcgctggc gggcaatctc tgccgctgca
     3601 ccggctacat tcctattttc cgcgccgttc agaaggccgc gaagacgatg cgcgagctgg
     3661 ggcatttgcc cggcggcgcg cgctgacagg gagggaaccg cgatgaaaca gactgtgacg
     3721 cgcaacggcg tcggcgcgtg gaccacccgg aagtacgact tggagaaggc cgccggaact
     3781 ctcgccttca ctgacgatct ccgtttcggt cccgagctgc tccacgcccg ggctttccgc
     3841 tccaccaagc cccatgcgga gatcctctcc gtcgacgtct ccgaggccat gaaggtcaag
     3901 ggcgtagtaa aggtgatcac cggcaaggac ttcatccacc gtttcggcct ttaccttatg
     3961 gaccgtacgc cgctggccat cggaaaggtc cgctacgtcg gcgagcccgt ggcgctcgtg
     4021 atcggcgaga ccgaggaggc cgccgaggag gggatgctca aggtcgccgc gaagtacaag
     4081 gacctgcccc ctatcttcga tcccgtcaag gccgctacgg acaactccgt cctcgtccat
     4141 cctgaacttg agacatacga ccacgtgccg ttcatcacgc cgttccccgg gacgaacatc
     4201 gccaactggt tccgtattcg acgcggcgac gtggacaagg ccttcgccga ggcggagcat
     4261 gtcgtcgaag aagccgtcaa ctgtccgcag atcgcccacg gttttcttga gccgcactgc
     4321 tgcatctgca aggaggaccc ggccac
//
LOCUS       DAOPKU010000223         4321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_22269_length_4321_cov_3.657759, whole genome shotgun sequence.
ACCESSION   DAOPKU010000223 DAOPKU010000000
VERSION     DAOPKU010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22269_length_4321_cov_3.657759"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="PK849_07905"
     CDS             <1..1302
                     /locus_tag="PK849_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPE66089.1"
                     /translation="EKIFCSRRKLPELEKALSSLSYAPEILVFEECLLPPGANASGDH
                     FLERDDDDTALIIYTSGTTGSPKGAMISFANIAANVRAVSEAAPIFTPESRVLVLLPI
                     HHVLPLGGTILGPLFVGACCVFSPSIGSRDILETLQHNGVTVVVAVPRFYSMLIKGIR
                     EKIQESIAARTFFALAKKVDNPRLSKFLFKKVHERFGGKIRFLVCGGAAIDDDVASDF
                     KALGFDMLTGYGMTETAPLISFTHPGTLRIGASGYVCPAVEVRIVDGEITVRGRHVMK
                     GYYNRSEETAAMIRDGWLHTGDLGFVDEDGFLFVTGRKKDIIVLSNGKNINPEEIEAK
                     LLSGFNAVKEVAVCLYNGVLHAIILPDFEKIAESGKRPSEEFFRHEVIGEYNHRVAST
                     KKIMKFTLVQDELPKTRLGKIRRFQLPELLALIAEAESKAN"
     gene            complement(1397..2704)
                     /gene="glgC"
                     /locus_tag="PK849_07910"
     CDS             complement(1397..2704)
                     /gene="glgC"
                     /locus_tag="PK849_07910"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPE66090.1"
                     /translation="MQTGKYGRTMGMVLAGGKGERLMPLTKYRAKPAVHFAAKYRIVD
                     FALSNLVNSGIFAIYVLVQFKSQSLNEHIERGWQFGGALRGRDFFITLVPAQMWRGEH
                     WFQGTADAVYQNLHLVTLYDADRICIFAADHIYKMDVDQMLEYHMNKQADVTVAANVV
                     PSSEASAFGCIHVDDSGRIIDFIEKPANPPEIPGKPGFSYVSMGNYIFEREILEESLI
                     EDAHLPTSHDFGRDIIPKLVPKARIFAYDFSTNVLPGKTRSFWREDKPYWRDVGTIKA
                     YWEAHMDLLDHDSEMTFYNPQWPIRTVSYADPPSYVCPVKGFSCYVQSTLSAEGSRVL
                     GSRVESSVLSRNCVIMPGSDIEQSIIGEGVVVGENCRLKKVIIDSHNVIPPNTEIGFD
                     PEHDRERYAVDPNSGIITIGMPKMQLRKSIEQPKEGFDWSSFS"
     gene            complement(2777..4243)
                     /locus_tag="PK849_07915"
     CDS             complement(2777..4243)
                     /locus_tag="PK849_07915"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HPE66091.1"
                     /translation="MATLHASREIKVLHVSPEAVPFCKVGGLADVAGSLPPSLRKSGT
                     DCRLLLPAWDGVLDTAHKRGLKLVRLPRSVDVAIRWKVYNGTLWMSSGEDFPVYFLES
                     PFFEKKQIYPQQLDPESVLPFVFLSLAALELADATEWDADVLHCHDWGTAPLISALLW
                     HRYYRFMFSQYKTVFTIHNLAHQGLLPLHSLRDWGLVDDAFTLNGLEFYGGANLMKGA
                     ITGASAVTTVSPTYSHEIQTENGGVGLGGLLRSQNSKIRGILNGLDTEYWNPATDSLL
                     FQNYSPDDLSGKTRCRNALLDRLGWKEDGRPLFTSVGRMVDQKGFDILLPAIPRMVEW
                     GCRLFLVGSGQREYETAIQEAAAKWPDAVASFVGYDEVIAHQAYAAGDFFLMPSKFEP
                     CGLSQLISLRYGALPIVRSVGGLADTVFEYGSPEGNGFLFSEYSAEGLLSAVGRSLDV
                     FRNDAAGYRSVQKKAMRSDFSWDRSAPLYIDLYRSLLS"
BASE COUNT          977 a         1145 c         1233 g          966 t
ORIGIN      
        1 gagaaaattt tttgttcccg caggaagttg cctgagctcg aaaaagcact ttcctctctc
       61 tcctacgctc cagaaatttt ggtatttgaa gaatgtcttc ttcctccggg agcgaatgcc
      121 tccggagatc atttcctcga acgcgacgac gatgatactg ctctgataat ttatacatcg
      181 ggcaccaccg gaagcccgaa gggcgccatg atctccttcg ccaatattgc ggcgaacgtc
      241 agggcggtga gcgaagccgc tcctattttt actcctgaat cgcgggttct ggtgttgctg
      301 cctattcatc atgtgcttcc tctgggaggg acaatcctcg gacctctctt cgtgggagcg
      361 tgctgcgtct tttcgccttc gataggttcc agggatatac tggagacgct ccagcacaac
      421 ggagtcacag tggtcgtcgc tgttccgcgt ttctacagca tgctcataaa gggcatccgg
      481 gagaagatac aggaaagtat tgccgcgcgg acttttttcg ctctggcaaa aaaagttgac
      541 aatccgaggc tctccaaatt tctgttcaaa aaggtccatg agcgttttgg aggaaaaatc
      601 cgttttctgg tctgcggcgg cgcggcgatc gacgacgatg ttgcaagtga ctttaaagcg
      661 cttggattcg acatgcttac cggctacggg atgacggaga ctgcgccgct catttcgttc
      721 acgcatccgg gaaccttgag gatcggggcg agcgggtacg tctgtcccgc cgtcgaagtg
      781 cgtatcgtcg acggtgaaat caccgtcagg ggacgtcatg taatgaaggg gtactacaac
      841 cgctctgaag aaacagccgc catgatccgc gacggctggc tgcacacagg agatcttgga
      901 ttcgtggatg aggatggctt tcttttcgtt accggccgaa agaaggatat tattgtgctt
      961 tccaacggga agaatatcaa tcccgaagag atcgaggcga agctgctctc cggatttaat
     1021 gcggtgaagg aggttgcggt ctgtctttat aacggggtgc tgcacgccat cattctccct
     1081 gattttgaaa aaatcgcaga aagcggcaag cggccgtccg aagagttctt tcgtcacgag
     1141 gtgatcggcg agtacaacca tcgggttgcg tcgacgaaga agataatgaa gtttaccctg
     1201 gtacaggatg aactgccgaa aacacgcctc ggaaagatca ggaggtttca gctgccggag
     1261 ctgctcgccc tgattgcaga ggcggagagc aaggcgaact gataccggcg caccggaata
     1321 attcgcagct ttagtgttct cgaagaggaa gggaagaaca ggggggcgtt tgaacgcccc
     1381 cctgttctgt ctgctgttaa ctgaagcttg accagtcgaa gccttccttc ggctgttcga
     1441 tggacttgcg aagctgcatc ttcggcatgc cgatggtgat gatgcctgaa ttcgggtcca
     1501 cggcgtaccg ttctctgtca tgctccggat cgaatcctat ctcggtgttg ggcgggatca
     1561 cgttgtggct gtcgatgatg acctttttaa gacggcagtt ttccccgacg accactccct
     1621 cgccgatgat cgattgttca atatccgagc ccggcataat cacgcagttg cgggacagca
     1681 cggacgactc gactctggat ccaaggacgc ggcttccttc cgccgaaagg gtgctttgaa
     1741 cgtagcagga gaatcctttc accgggcaga cgtacgacgg agggtcggcg taggagacgg
     1801 tccgaatagg ccactgcggg ttatagaatg tcatctcgga gtcatggtcg agcagatcca
     1861 tgtgcgcctc ccagtatgcc ttgatggtcc caacgtcgcg ccagtagggt ttgtcctctc
     1921 tccagaagga gcgcgtcttt ccgggaagga cgttcgtcga aaagtcgtag gcaaaaattc
     1981 gggctttcgg gacgagtttt ggaatgatat ccctcccgaa atcatggctt gtggggagat
     2041 gagcgtcctc aatgagggac tcttcaagga tttcccgctc gaagatatag ttgcccatgg
     2101 agacgtatga aaaccccggc ttacccggga tctccggcgg atttgccggt ttttcgatga
     2161 agtcgataat gcgtcctgaa tcgtcgacgt gaatgcagcc gaaggcggaa gcctccgacg
     2221 acggtacgac gttcgcagcc acggtaacgt cggcctgttt gttcatgtga tattcgagca
     2281 tctggtccac atccatcttg tagatatggt cggcggcaaa gatgcaaatt ctgtccgcat
     2341 cgtagagcgt gacaagatgg aggttttgat agacggcgtc ggcagtaccc tggaaccaat
     2401 gctcgccgcg ccacatctgc gccgggacga gcgtaatgaa aaagtcgcgt ccgcgaaggg
     2461 cgccgccgaa ctgccatcct ctttcgatgt gttcgttcag ggattggctt ttgaactgca
     2521 ccagtacata aatagcaaaa atgccgctgt ttaccagatt ggaaagcgca aaatcaacga
     2581 tacggtactt tgctgcaaaa tggactgcgg gttttgcccg gtatttcgtc agcggcataa
     2641 gccgctcccc tttccctcca gcaagcacca ttcccattgt acggccgtat tttcctgtct
     2701 gcatgctgtt tcccccctcc ttctcaagat ggcgttgacg aatgtcgcac tgcgttttcc
     2761 cgtctgtagt tcacgctcag gacagaagtg aacgatagag atcgatgtag agcggcgccg
     2821 aacgatccca ggaaaagtcg gaccgcatcg cttttttctg tacggaacga tatcctgccg
     2881 cgtcgttcct aaaaacgtcg agcgacctcc ccacggcgga gagaagacct tccgccgagt
     2941 actcggagaa aagaaagccg ttgccctcgg gagagccgta ctcgaagacc gtatcggcaa
     3001 gccctccgac tgaacggaca atcggaagag cgccgtagcg cagcgagata agctgcgaga
     3061 gaccgcacgg ttcaaacttg gagggcatga gaaaaaagtc tccggcggcg taggcctggt
     3121 gtgcaatcac ttcgtcgtat cctacaaatg atgcgacggc gtccggccat tttgcggcgg
     3181 cttcctgaat tgcggtctcg tattcacgtt gcccgctccc gacaagaaag agtctgcacc
     3241 cccattcgac catcctcggg atcgcgggca agagaatatc gaaccccttt tgatctacca
     3301 tcctgccgac cgaggtaaag agcggccgac cgtcttcttt ccatccgagc cggtcaagaa
     3361 gagcattccg gcatcgggtt ttccccgaga gatcatccgg agaatagttt tgaaaaagga
     3421 gactgtcggt ggccgggttc cagtactcag tatccaggcc gttgagaatg ccgcgtatct
     3481 ttgagttttg actcctgaga agccccccga gaccaacacc gccattctcg gtctggatct
     3541 catgagaata ggtcgggctg acggtagtta cagcggaagc gcctgtgatc gcgcccttca
     3601 tgaggtttgc gcccccgtag aactccaatc cgttgagcgt aaaagcgtcg tcgacaagtc
     3661 cccagtcgcg gagcgagtgg agggggagaa ggccctgatg cgcaagatta tgtatggtga
     3721 agactgtctt gtactgcgaa aacatgaacc tgtagtacct gtgccagagc agcgcgctga
     3781 tcagcggggc tgttccccag tcgtgacagt gcagcacgtc ggcatcccat tccgtggcat
     3841 cagcgagttc aagcgccgcc agggaaagaa agacaaacgg aagcacggac tccgggtcaa
     3901 gctgctgcgg atagatctgt ttcttctcga agaacggaga ctcgagaaaa tagacgggga
     3961 aatcctcgcc ggaggacatc catagcgtgc cattgtacac cttccagcgg atggcgacat
     4021 cgacgctccg aggaagccgg acgagtttca ggccgcgctt gtgcgcagta tcaagaacgc
     4081 cgtcccacgc gggaagaagc aggcggcagt ccgtaccgct ctttcggagg gaagggggca
     4141 gcgagccggc aacgtccgcc agccctccca ccttacagaa cgggacggct tcggggctta
     4201 cgtgaagaac cttgatttct cttgaagcat gaagtgtcgc cactgcggat cacatccccc
     4261 ggaaactgat gttgtacgcc ttttcgcagt actcgcggac cacgcgatgc gtattgaaaa
     4321 a
//
LOCUS       DAOPKU010000224         4317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_22300_length_4317_cov_3.739090, whole genome shotgun sequence.
ACCESSION   DAOPKU010000224 DAOPKU010000000
VERSION     DAOPKU010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22300_length_4317_cov_3.739090"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PK849_07920"
     CDS             <1..316
                     /locus_tag="PK849_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPE66092.1"
                     /translation="GNAARGMTLCALHNGGGVGIGKSVNGGFGLVLDGAERTDEIIRS
                     AMMWDVLGGVARRAWARNPNAMEVSREVNRNYPDSYHITLPYSVEEESVKNAVDALFK
                     AK"
     gene            355..948
                     /locus_tag="PK849_07925"
     CDS             355..948
                     /locus_tag="PK849_07925"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HPE66093.1"
                     /translation="MRVIRRTPPDYRTMPWKNGGGITHEMFICPEDATLQKGDFLWRL
                     SSANVGESGPFSTFPGYDRVLFLLSGNGLELTVNGETVPLRSPFDSLRFRGEHDASCM
                     LTDGPVTDFNIFCRREILTCEYRHLFSKKDIERLFLPGDTTILLCLQGEATAEIEGKH
                     PLKEMEMLVVTDHSWGQPLTLQYGEKARLLFITLTKR"
     gene            complement(1050..2117)
                     /locus_tag="PK849_07930"
     CDS             complement(1050..2117)
                     /locus_tag="PK849_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPE66094.1"
                     /translation="MSGTMEEHNLKKEGYEMKQVRVFSLLLGIAFVVSGMFVCAGPLA
                     AQEYTLQLAGAYPSTGETPRALATEKIKELIEKKSNGKIKVQIYLDNQLGGDREILEG
                     CQLGDIAMVSQTTAPQVGFIPELAIFDIPMLYENLDVARKALSGTFRAKLEEWYDRAG
                     LKLLLFEPIYYRETTTSKPINTVADFGGVKIRTMENEYHMAFWKALGANPTPLSFAEL
                     YVALQQGTVDAQENPYEIIWSSKFYEVQKYLVNTHHIAFILSININKGIYDGMSDEYK
                     KIIDESMKEASEYLFDLAKAKNDAMLASLKQSGMTIIDPDPALQAELKKAAAETEKLI
                     RKNVGDAVVDEVLKSAEEAKK"
     gene            complement(2243..2611)
                     /locus_tag="PK849_07935"
     CDS             complement(2243..2611)
                     /locus_tag="PK849_07935"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE66095.1"
                     /translation="MSNFILNNFAGNKYIFLIALNIILLVLGMFMDVGPAIAILAPMM
                     LAAATALGISPVHLGVIMVINLAVGMVTPPFGVDLFVAAPLINEHVMKVGIKAIPFII
                     AFIFALILITFIPQMSLLFL"
     gene            complement(2608..3531)
                     /locus_tag="PK849_07940"
     CDS             complement(2608..3531)
                     /locus_tag="PK849_07940"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE66096.1"
                     /translation="MVLTLFIILIGGLALGVPIAVALGFATIFPGLTNPAFPASLAYV
                     IRNIVSALDSTPMLAIPLFIFSGNIMTKGKISDKLFNFFAYFIGDFPAGIPITAIFTC
                     LFYGAISGSGVATTAAVGGMAIPFLVSLGYDKVYSAALVATAGSLGVIIPPSIPFVTY
                     GVITGVSIGSLFIAGIIPGLVIGLSLMGYALFYAKVHGEDRKLISEKVKALRSGGLLR
                     LFRESFWALLTPVIILGGIYSGIVTPTEAAAVSVFYALLVCLFVYKSIAFKELGTILR
                     DTVKSYAPIVVLLSLAIVFGRVLALLQAPPF"
     gene            complement(3534..4022)
                     /locus_tag="PK849_07945"
     CDS             complement(3534..4022)
                     /locus_tag="PK849_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE66097.1"
                     /translation="MKILRWLDQHFEEYVLSGLLVVIAVVMMLQVIMRYVFNASLSWA
                     EEASRYAFVWSALISIGYTIKENSILKVDTLVESLPAGLKHLLVTSINVSVTLFFGYL
                     FINSIPAIQRVIRTGQTSPALKIPLGWIYFAAIAGFFLATVRSVQKTGQDFGVTNRKK
                     EA"
     gene            complement(4129..>4317)
                     /locus_tag="PK849_07950"
     CDS             complement(4129..>4317)
                     /locus_tag="PK849_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66098.1"
                     /translation="ETPSNTGLLENTFPLFENNPGRFPFFTSSEKPLTPLSLSDIYRI
                     NPLNGKCCRRVEISGEPL"
BASE COUNT         1109 a         1108 c         1137 g          963 t
ORIGIN      
        1 cggcaacgcg gcccgcggca tgacgctctg cgcgctccac aacggcggcg gcgtggggat
       61 cggcaagtcg gtcaacggag gattcggact cgtcctcgac ggggcggagc gcaccgacga
      121 gatcatcagg tccgcgatga tgtgggacgt cctcggcggc gtagcccggc gcgcctgggc
      181 ccggaacccg aacgcgatgg aggtcagccg ggaagtgaac aggaactacc cggacagcta
      241 ccatatcacg cttccgtaca gcgtggagga agagagcgtg aagaacgccg tcgacgcgct
      301 ctttaaggca aaataaaggc acacactacg agagaataac gagaggtgaa gagtatgcgc
      361 gtcatacgaa gaactccccc ggactatcgg accatgccgt ggaaaaacgg cggaggcatc
      421 acgcatgaaa tgttcatctg cccggaggat gcaacgcttc aaaaaggaga tttcctttgg
      481 cggctgagtt ccgcgaatgt gggcgaatcc ggtccgtttt caacgtttcc gggatacgac
      541 cgtgtcctct tcctcctctc cggaaacggc ctggaactca ccgtaaacgg agagacagtc
      601 ccgctccgct ctcccttcga ctcgcttcgg ttcagaggcg aacacgacgc gtcctgtatg
      661 ctgaccgacg gtccggtgac ggatttcaac attttctgca ggagggagat cctcacgtgc
      721 gaataccgtc acctcttctc gaagaaggat attgaacgtc tttttcttcc cggggacaca
      781 acgattcttc tttgtctcca aggagaggct acggcggaga ttgaaggaaa gcatcctctg
      841 aaggagatgg aaatgcttgt cgtcacggac cactcttggg gacagccgtt gacccttcaa
      901 tacggggaga aggcacgcct tcttttcatt actttaacaa aacgctaaaa acgtgtaagg
      961 aaaaactcca tgcgctgaaa tagctgaaag aagaagcccg gatcccaacg acccgggctt
     1021 cttctttcag aaaaacaagg agcgaatcgc tattttttcg cttcttcggc tgacttcaac
     1081 acttcatcga cgacagcgtc ccctacattt ttacggatca acttttcggt ttccgcagca
     1141 gcctttttca attccgcctg aagggcggga tcaggatcga tgatggtcat cccgctctgt
     1201 ttcagggaag ccagcatcgc gtcatttttc gccttggcaa ggtcaaaaag atactccgac
     1261 gcttccttca tactttcgtc gatgatcttt ttgtattcat cggacatgcc atcatagata
     1321 cccttgttga tgttgatgct cagaataaac gctatgtgat gggtattgac gaggtatttt
     1381 tgaacttcgt agaatttgct ggaccatatg atctcgtagg gattttcctg agcatcgacg
     1441 gttccctgct gaagagcgac gtacagctcc gcgaagctca gaggggtagg attggccccc
     1501 aaagctttcc agaaagccat gtggtattcg ttctccatgg tgcggatctt gactccgcca
     1561 aagtcggcaa cggtattgat gggtttgctg gtggtggttt cccggtaata gatgggttcg
     1621 aaaaggagga gcttgagtcc cgccctgtcg taccattcct cgagctttgc ccggaacgtc
     1681 ccggaaagcg ctttcctcgc tacatccagg ttttcataca gcatggggat atcgaaaatg
     1741 gcgagctcag gtatgaatcc tacctgggga gctgtcgtct gagagaccat ggcaatgtcg
     1801 cccagctggc atccttcgag tatctcccgg tcgccgccaa gctgattatc gaggtaaatc
     1861 tgcaccttga tcttgccgtt cgatttcttc tcgataagct ccttgatctt ttccgtagcc
     1921 agcgctctgg gagtttctcc ggtggatgga taggccccgg ccaactgcag cgtatattcc
     1981 tgggccgcca gcggacctgc acacacgaac attcccgaca caacaaaagc aattcccaat
     2041 agaagcgaaa acaccctgac ctgcttcatt tcatacccct cctttttcaa gttgtgctct
     2101 tccatggttc ccgacatacc gaacagcctt ctccatatcg agctgaaaag gtctttgtct
     2161 ttttcacctc ccttgagagc gtagaatgga tgaaaaactc ccgctttgta ttgtcaaaaa
     2221 aacgaccgtc cagatcccta tcctacagga acaagaggct catctgcggt ataaaggtaa
     2281 tcagaatgag cgcaaagatg aacgcgatga taaaggggat tgcttttatg cccactttca
     2341 tcacatgctc attaataagc ggcgccgcca caaaaagatc cacgccgaag ggaggcgtga
     2401 ccatgccgac tgcaaggttg atcaccatga taactcccag gtgaacgggg cttataccaa
     2461 gggcagtggc ggcggcgagc atcattggcg caagaatggc gatggccggt cccacgtcca
     2521 tgaacatgcc caggaccagg agaataatat tgagcgcgat gaggaaaata tacttgttcc
     2581 cggcaaaatt attcaggata aagttgctca aaacggcggg gcctgtagca gggcaagaac
     2641 ccggccgaac acaatagcca gggacaggag aacgacgatg ggggcgtagg atttgacagt
     2701 gtctctgagt attgtcccca gttccttgaa tgcaatactt ttgtagacaa aaagacatac
     2761 cagaagggcg tagaagacgg aaacggcggc ggcctccgtg ggggtcacga tcccgctgta
     2821 gatcccgccg agaatgatga ccggagtgag caaagcccag aagctctctc tgaacagccg
     2881 caacaaaccg cctgaacgaa gggctttcac tttttcagaa atgagttttc tgtcttcacc
     2941 gtgcaccttg gcgtagaaaa gagcatagcc catgaggctg agtccgatga cgagtccggg
     3001 aatgatcccc gcgataaaga gactcccgat ggatacgccg gtgatcactc cgtaggtgac
     3061 gaaaggaatg ctgggaggaa ttatgacacc gaggcttccc gccgtggcca ccagcgcggc
     3121 gctgtagacc ttgtcatacc ccagtgagac caaaaagggg attgccatgc ccccgacagc
     3181 ggcggtcgtg gccacaccgg atccggaaat cgcaccgtaa aacaggcagg tgaagattgc
     3241 cgttatgggg attccggcgg gaaaatcgcc aataaaatag gcaaagaaat taaacagctt
     3301 atctgaaatc ttccccttgg tcatgatgtt tcctgagaag ataaaaagag ggatggccag
     3361 catgggagtg ctgtcgagcg cgctcactat attccggatg acataggcca gcgaagcggg
     3421 gaacgccgga ttcgtcaatc cggggaagat cgtcgcaaaa ccaagcgcca ctgcaatagg
     3481 aactccgagg gcaagaccgc ctatcaggat aatgaagagt gtaagaacca tctttacgcc
     3541 tcctttttcc tgttcgttac gccgaaatcc tgccccgttt tctggacgga cctcaccgta
     3601 gccaggaaga agcccgcgat agcggcaaaa tagatccaac ccagggggat tttcagggca
     3661 gggcttgtct ggccggtcct aatgactctt tgaatcgccg ggatcgaatt gataaagaga
     3721 tagccgaaaa aaagagttac agatacgttg atcgaagtga ccaggagatg tttcaaacct
     3781 gcgggcaggg attcaacaag agtgtcgacc ttgagaatac tgttttcctt gattgtgtac
     3841 ccgatgctga tgagggctga ccagacgaat gcataacggg aggcctcctc ggcccatgac
     3901 aaagacgcgt taaaaacata gcgcatgatg acctgaagca tcatgacgac ggcaatgaca
     3961 acgagcagac cgctcaaaac atactcttca aaatgttgat ccaaccatct cagtattttc
     4021 aatagcttca cctccggcat ggtttcaaaa gcggcccacc tggggaatct atcattgcat
     4081 ccctcggggg atactcttat ttcctaaata gtacagcata atagcctttt aaagaggttc
     4141 tccggaaatt tccactctac ggcaacactt gccatttagt ggatttattc tatatatatc
     4201 agacaaagag agcggtgtca agggtttttc tgatgatgta aaaaagggga acctccctgg
     4261 attgttctca aacagaggga atgtgttttc aagcaacccg gtgttcgatg gtgtttc
//
LOCUS       DAOPKU010000225         4280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_22582_length_4280_cov_4.424852, whole genome shotgun sequence.
ACCESSION   DAOPKU010000225 DAOPKU010000000
VERSION     DAOPKU010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22582_length_4280_cov_4.424852"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..105
                     /locus_tag="PK849_07955"
     tRNA            16..105
                     /locus_tag="PK849_07955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Ser,seq:cga)
     gene            168..255
                     /locus_tag="PK849_07960"
     tRNA            168..255
                     /locus_tag="PK849_07960"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Ser,seq:gga)
     gene            446..1915
                     /locus_tag="PK849_07965"
     CDS             446..1915
                     /locus_tag="PK849_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66099.1"
                     /translation="MLGMIGSSESLPEEQRTTLTALAHFHKTRVLSGLPSPQRLASLH
                     RFLVRRFIDDTTFSLTQEQYELVDSGAYSSASSLSSVQRSDVCAALTLTEWIEDTRAA
                     FEEGKEDYAVSHVTKAIASSEARLNALGGRIRTMLPLLFTTLGSVTSDGGLFIMPKNR
                     ADMLAGYFLESLDETARATMRFLEQEPPAEEPSEDEENRKKKKERKLESFPKLMEGIR
                     RLKTIAKDEDGSDLVVDRLLSSIDMLFLESLTAYAYKKSALEVVRRKKKLRQEVNDTP
                     FSPEQALLEIMKQHLERIYAVQERACKAFSEENAPEKSGYTPFLLPDSVEYLDILDEE
                     EIQREKKKKAGDLSFERAFLEILSADRGLFTLPRIRSRGLPGILFLPGNAVGAYDKKA
                     DFFLLPFSALRERAFEHLLNAFASFRWAWDDSGKVLNLCADLVGSRGAKPQLELEAAF
                     RRAYSSWLQSEIRGRAVDEHGESIKKALRPEGNFARFGE"
     gene            complement(1967..2965)
                     /locus_tag="PK849_07970"
     CDS             complement(1967..2965)
                     /locus_tag="PK849_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPE66100.1"
                     /translation="MREILFVSNTGNTTVVIFFVLFTVILVMSGIKIVPQASRVIVER
                     LGKFHKILEPGINFIFPVLDRRKATSWIVGGEIRNTTLIDIREQVFDFPKQNVISHDN
                     VVMEINALLYFQINDPFKAVYEIANLPLALEKLTQTSLRSVMGEMDLDEIFSKRAEIN
                     ERLRVILDEASDAWGAKVTRVEIQDVNPPASVQAAMQRQMEAERSRRAMVTEAEGKKT
                     AQILEAEGHKEAQINRAEGEKQAVILDAEGRARARIRMGEAEAEAVVKVASAVKIAGG
                     DPTQYLIALKYMDTLSSVASGNENKVVYLPYEATAILGSLGGMKELFSKDKTQTNA"
     gene            complement(3042..3518)
                     /locus_tag="PK849_07975"
     CDS             complement(3042..3518)
                     /locus_tag="PK849_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPE66101.1"
                     /translation="MFELVTNWQLWVILGFALLIVEMFTPGFVLACFAIACIPPAIIT
                     YFGDFDLRAQLAVFGVSTLFIFFFMRPAVLRFLSNRKDARTSNAEALIGKKGIVIKTI
                     SPDAAQGGYVKVDGKTWWAFGPEGIEIGEGAGVVVVKVRGASLDVQFEENESSGEE"
     gene            complement(3576..4178)
                     /locus_tag="PK849_07980"
     CDS             complement(3576..4178)
                     /locus_tag="PK849_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPE66102.1"
                     /translation="MQWTAFFTYILITCNTPGPNVVLAMNTARIHGLRKTLPLIAGMM
                     SGLLLLMTFCAIFNLLLARLLPTLLPVLRTGGTVYILWLAWKIAFPKRNSLENAPAED
                     APDFRHGFVLQFLNPKVILLGLTTLSTFILPWTTSKTWLFGCGVFLTLACSNGVFLWS
                     LLGAAQKRLMTEQGRFWDYLMGGLLAWCAFSVSGLKELFF"
BASE COUNT         1055 a         1106 c         1074 g         1045 t
ORIGIN      
        1 ttattcgggt cgtgcggaga ggtggccgag tggtcgaagg cgattgactc gaaatcaatt
       61 gggcggcttg ccgtcccgtg ggttcaaatc ccaccttctc cgccaataaa aaagtttttc
      121 cggtctggca aacttggaaa actgtgatat aatacctgcc gttatgcgga gaagtggccg
      181 agtggtcgaa ggcgggcgct tggagagcgt ctgaaccgtg aggttccgtg ggttcaaatc
      241 cctccttctc cgccattttt ttcaatatct tcccccccta ataaatcttt ctcattcact
      301 taaaaaacga atgtcttgtc tgttcgcctg ctctgatgta tcctcttacc agagacgtac
      361 cttagagatc ggagatgttt cagatgcaga caaaaacaac tctttcctcc gggagctttt
      421 ttatttcgcg aactggggcc gatatatgct cggcatgatt ggttcctccg aatcgctacc
      481 tgaagagcag cgtactacat tgactgccct tgcccatttc cacaaaacaa gggttctttc
      541 cggcttgccc tctcctcaac gtttagcgtc tctccacaga tttctcgtac gccgttttat
      601 cgatgatact acgttctcat tgactcagga acagtatgag cttgtcgatt caggagcgta
      661 tagctctgct tcgtctctct cttcggtgca aaggagcgac gtgtgtgcgg cgctcactct
      721 gactgaatgg attgaagaca cgcgagctgc ctttgaagaa ggaaaagaag attatgccgt
      781 ttcacacgta acgaaagcga tcgcttcatc cgaggcacgt ttgaacgcgc tcgggggcag
      841 aattcgcaca atgctgccgc tccttttcac aacccttggg agcgttactt ccgacggggg
      901 cctttttatc atgccgaaga accgtgcaga tatgctcgcc gggtattttt tagaatctct
      961 tgacgaaaca gcccgggcga caatgcgttt tctggagcag gagccgcctg cggaggagcc
     1021 ttcagaggat gaagaaaaca ggaaaaagaa gaaagagcgg aagctggaaa gctttccgaa
     1081 gttgatggag ggcattcgac ggctgaagac tattgccaaa gatgaggacg gcagcgattt
     1141 ggtggtagac agattgcttt cgagcatcga tatgttattc cttgaatcac ttactgcgta
     1201 tgcctacaaa aaaagcgctt tggaagttgt gcggcgaaaa aaaaagttac ggcaagaagt
     1261 gaacgatacc cccttctcac ctgaacaggc gcttcttgaa atcatgaagc aacatctgga
     1321 gagaatctat gctgttcaag agagagcgtg caaagcgttc tcagaggaga acgcaccgga
     1381 aaaaagcggc tatactcctt ttctcctccc tgattccgtc gagtatctcg atatactgga
     1441 tgaagaagaa attcaacgtg aaaaaaagaa aaaagcagga gatctgagct ttgaaagagc
     1501 attcctcgag attctttctg ctgacagggg gctttttact ctcccgcgaa ttcgttcccg
     1561 gggtcttccc ggcattctct ttcttcccgg aaacgctgtc ggtgcatacg ataagaaagc
     1621 agatttcttc ctccttcctt tttccgcgct cagagaacgg gcattcgagc accttttgaa
     1681 tgcgtttgcc tctttccgct gggcttggga tgacagcgga aaagtcttga acttgtgcgc
     1741 tgatcttgtg ggcagcaggg gagcgaagcc gcagctggaa ctcgaagctg cgtttcgcag
     1801 agcctattcg tcatggcttc agtctgaaat tcgtgggaga gcagtcgacg aacacggtga
     1861 atcgataaaa aaagcgctca ggccggaagg aaacttcgcg cgtttcggag agtaattgct
     1921 tcagacagaa gagagagggt gaataccccc tcccttctgt actgttttaa gcgttcgttt
     1981 gtgttttgtc cttagaaaaa agttctttca tgccgccgag cgagccgagg atcgctgtag
     2041 cttcataagg caggtagaca accttgttct catttccgga tgccactgag gaaagggtgt
     2101 ccatatattt aagagcgatg aggtactgcg tgggatcgcc tcccgcaatt ttcacggccg
     2161 aggctacttt caccacagcc tcagcctcgg cttctcccat gcgaattctg gcccgggcgc
     2221 gtccttccgc atcaaggatc accgcctgtt tttctccctc agcgcgattg atttgcgcct
     2281 ctttatgccc ttccgcctca agtatctgcg cggttttttt cccttccgcc tcggtgacca
     2341 tggcccgcct ggagcgttcc gcctccatct ggcgctgcat tgcggcctga accgaggcag
     2401 gcggattcac gtcctgaatc tccactctcg tcacctttgc cccccaagca tcgctggctt
     2461 cgtcgaggat aacacggaga cgttcgttaa tttccgcgcg cttactgaat atctcgtcaa
     2521 ggtccatctc tcccatcacg ctcctgagag atgtctgcgt cagtttttcc agagccagcg
     2581 gcaggttggc aatttcgtac accgctttga acggatcgtt gatttggaaa tagaggagag
     2641 cgttgatctc catcacgaca ttgtcatgcg agataacgtt ttgctttggg aagtcgaaaa
     2701 cctgctccct gatatcaatg agcgttgtat tccgtatctc ccctccgaca atccaggaag
     2761 ttgcctttct ccggtcgaga acggggaaga tgaagttaat gccgggctcg agtattttat
     2821 gaaactttcc gagacgttcc actatgacac ggcttgcctg aggaacgatc ttgatgcccg
     2881 acatcaccag gatcacggta aacagaacga agaagatgac gaccgttgtg ttgcccgtgt
     2941 tgctgacgaa cagaatctct ctcattgttt tcacactccc cgtttatcgg atgtgttcct
     3001 gaaaattatt caagaatgat ctagtcagaa caccctgtgc cttactcttc acctgatgac
     3061 tcgttctctt cgaactgaac gtccaggctg gctccccgaa cttttacgac gacgaccccc
     3121 gccccctctc cgatttcgat cccttcggga ccaaacgccc accaggtttt cccgtccacc
     3181 ttcacgtatc ccccttgagc agcgtccggc gagatggtct ttattacgat gccttttttc
     3241 ccgatgagcg cctcggcgtt cgacgtgcgg gcatctttcc tgttcgacag aaatctgaga
     3301 acggcaggcc gcatgaagaa gaagatgaaa agagtagata cgccgaagac agcgagttga
     3361 gcacgcagat caaagtctcc aaagtatgtg attatcgccg gagggatgca ggcgatggcg
     3421 aaacatgcaa gaacgaagcc cggagtgaac atctcgacga tcagcaaggc aaatcccaga
     3481 atcacccaaa gctgccagtt tgtcacaagc tcgaacattt ttctcacctc gactttctcg
     3541 aattgaacaa cgtaccctct ccgatgaaaa aggcatcaga aaaacaactc tttcagcccc
     3601 gaaaccgaga atgcgcacca ggcaagcaat ccgcccataa gataatccca gaaacgtccc
     3661 tgttctgtca tcagtctttt ttgcgctgcg cccagaagag accagagaaa aaccccgttg
     3721 ctgcatgcaa gagtcagaaa gacgccgcaa ccaaaaagcc acgttttcga agttgtccac
     3781 ggaagaataa atgttgagag tgttgtcagc cccagaagaa taactttagg gttgagaaac
     3841 tggagtacaa acccatgccg gaagtcagga gcatcttcgg ctggagcgtt ttccagggag
     3901 tttcgtttgg ggaaggcgat cttccaggca agccagagaa tgtacaccgt gccgccggtc
     3961 ctgagcacag gaagaagagt aggcaacagg cgcgcaagaa gaaggttgaa gatggcgcag
     4021 aaggtcatca gaagaagaag gcccgacatc attccggcaa tcaaggggag cgttttgcgc
     4081 aatccgtgaa tcctcgcggt gttcattgcg agaactacat tcggccccgg agtgttgcac
     4141 gttatgagga tgtaggtgaa aaacgccgtc cactgcacaa gcgcacctcc aaagatgatg
     4201 tttggcgagg cagaagcccg ctttgatttt catattatac acgacgaacg attccagcag
     4261 agcagctcca caaacaagta
//
LOCUS       DAOPKU010000226         4273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_22640_length_4273_cov_4.979611, whole genome shotgun sequence.
ACCESSION   DAOPKU010000226 DAOPKU010000000
VERSION     DAOPKU010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22640_length_4273_cov_4.979611"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /gene="rplP"
                     /locus_tag="PK849_07985"
     CDS             complement(<1..391)
                     /gene="rplP"
                     /locus_tag="PK849_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPE66103.1"
                     /translation="MLMPSRVKYRKPFRRPLKGKAKGGNYVAFGEYGLQTLDCAWITA
                     RQIEATRVAISRKMKKGGKIWIRIFPDHPFTKKPLETRMGKGKGAPEFWVAPVKRGRI
                     LFEIAGVPRDIVEEAFRTASHKLPVKVR"
     gene            complement(398..1117)
                     /gene="rpsC"
                     /locus_tag="PK849_07990"
     CDS             complement(398..1117)
                     /gene="rpsC"
                     /locus_tag="PK849_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPE66104.1"
                     /translation="MGQKVHPIGFRLGVASEWESKWFASGKEYAKNLHEDIALRKYIA
                     KKWENAGISRIEIERVGHVIRFTVWTSRPGVVIGRGGAEIQVMKDELQAKTGAKIMIN
                     VQEIKNPDVVSQLVAVGVASALERRVSFRRAMKQSIFRAMKGGAKGIKISCAGRLGGA
                     EIARTEWYNEGRLPLSTIRADIDYGFAEAKTMYGVIGVKVWIFRDKENERPAQAPRQG
                     RGPRQDRSRGDRAPESSRGRG"
     gene            complement(1131..1463)
                     /gene="rplV"
                     /locus_tag="PK849_07995"
     CDS             complement(1131..1463)
                     /gene="rplV"
                     /locus_tag="PK849_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPE66105.1"
                     /translation="MEAKAVARQVRVSPIKVRRVLCLIRGKKVGEALVALRYSPQKSA
                     KLVSKVLQSAVANAEHNFGMDTDKLRIVAATADQGPVMKRFRPVSMGRAHPYRHRTSH
                     VTVIVAER"
     gene            complement(1477..1758)
                     /gene="rpsS"
                     /locus_tag="PK849_08000"
     CDS             complement(1477..1758)
                     /gene="rpsS"
                     /locus_tag="PK849_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPE66106.1"
                     /translation="MARSRKKGPYVDQKLLRKIEDMNEGTAKKVMKTWSRRSTIVPAM
                     IGHTIAVHNGRIHIPIYISENMVGHKLGEFAPTRKFGGHAGQERSSRVR"
     gene            complement(1772..2599)
                     /gene="rplB"
                     /locus_tag="PK849_08005"
     CDS             complement(1772..2599)
                     /gene="rplB"
                     /locus_tag="PK849_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPE66107.1"
                     /translation="MAIKKFNPYTPGRRFMTVQKNDDLTKKDPERSLLEPLHKKGGRN
                     NRGRITMRHRGGGAKRLYRVIDFKRNKLNVPGKVAAIEYDPNRNARIALIHYLDGDKR
                     YIIAPKDLNVGDKIVAGPESDIRPGNALKLKDIPVGTTVHNLELEPGRGAKLVRAAGT
                     SAQLMAKEGKYAYLRMPSGELRLILQECMATIGQVGNEDFENVSFGKAGKTRWLGRRP
                     MVRGMVMNPVDHPMGGGEGKSKSHKHPVSPWGTPAKGYRTRKKKPSDKMIVRRRYEK"
     gene            complement(2621..2917)
                     /gene="rplW"
                     /locus_tag="PK849_08010"
     CDS             complement(2621..2917)
                     /gene="rplW"
                     /locus_tag="PK849_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPE66108.1"
                     /translation="MNLVAHDIIIRPLITEKSSRLMELNKYSFEVHLSANKIQVRKAI
                     EEIFKVKVTAVHILNIHPKPKRMGAFIGKTRSWKKAIVSLAPGERIDFFEGASI"
     gene            complement(2914..3537)
                     /gene="rplD"
                     /locus_tag="PK849_08015"
     CDS             complement(2914..3537)
                     /gene="rplD"
                     /locus_tag="PK849_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPE66109.1"
                     /translation="MPTLKLVSFQGETVGEVQLSDGVFAAPVHVPAMHQVVVAQLANA
                     RLGTQSAKTRGEVRGGGKKPWRQKKTGRARHGSIRSPLWAGGGVTHAPKPRDYSQKVN
                     KKVRRLAIKSALSLKVREELMTVVKSFDIEKPSTKEMLAFLTAVKARKPLIILPEANE
                     AVLKSARNVPGAKVINMVNINVYDLLNAGTLILTPEVVEKLEEVYVG"
     gene            complement(3565..4191)
                     /gene="rplC"
                     /locus_tag="PK849_08020"
     CDS             complement(3565..4191)
                     /gene="rplC"
                     /locus_tag="PK849_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPE66110.1"
                     /translation="MSIGILGKKLGMAQIYNEQGQATPVTIIQAGPCPVVDLKTPSKD
                     GYSALVLGFGEPNPRKLNKSRKGLFEKAEVEPKKTLREFRVDSVDEYAVGQQIDVTLF
                     TEGETINVSGTSKGKGFAGVVKKYHFAGSNASHGASVVHRRGGSSGASSYPGRVFKGK
                     KMPGRMGGEKVTVKNLVVVAVDSDNHLLLVKGAVPGAKNSLVTLFKKG"
BASE COUNT          748 a         1313 c         1148 g         1064 t
ORIGIN      
        1 gacgcacctt gacggggagc ttgtgggatg cggtgcggaa tgcttcttcc acgatgtctc
       61 tgggaacacc cgcgatttca aagagaattc tgccgcgctt cacaggagcc acccagaatt
      121 ccggcgcgcc ctttcccttt cccattctcg tctccaaggg tttcttggtg aaaggatgat
      181 cggggaagat ccggatccaa attttgccgc cttttttcat cttacgtgag atggcgacgc
      241 gcgtggcctc tatctgtcgc gcggtgatcc aggcgcagtc gagggtctgg agaccgtact
      301 cgccaaaggc gacatagttt cctcccttcg ctttcccttt aagggggcgg cgaaaaggct
      361 ttctatactt cactctgctg ggcatcaaca tcgcgagtta cccccttccc cgggaagact
      421 ccggggcgcg atctccgcgg gaacggtcct gacggggccc gcgaccctgt cggggcgcct
      481 gcgcaggacg ctcgttctcc ttgtcccgga aaatccagac cttgacgccg ataacgccgt
      541 acattgtctt cgcctcggcg aatccgtagt cgatgtcggc gcggatcgtc gaaagaggca
      601 gacggccttc gttgtaccac tccgtgcggg caatttcagc tccgcccaaa cggccggcgc
      661 aggatatctt gattcccttc gctccgccct tcatggcgcg gaagatggac tgcttcatcg
      721 cacggcggaa ggacactctg cgctcaagcg cggatgcaac gcccactgcc acaagctgcg
      781 acacgacgtc agggttctta atctcctgga cgttgatcat aatcttcgcg cctgtcttcg
      841 cctggagctc gtccttcatc acctgaatct cggcgccgcc ccgtccgata actacaccgg
      901 gccgggaggt ccaaaccgtg aagcgaatga cgtgcccgac gcgctctatc tcgatgcggg
      961 agattcctgc attctcccac ttctttgcga tatacttccg aagcgcgatg tcctcgtgaa
     1021 gattcttcgc gtactccttg ccgctggcaa accacttgga ttcccactca ctggcaactc
     1081 ccagtcggaa tccgattggg tgaactttct gtcccacgag ttccccctcc ttaccgttcc
     1141 gcgactatca cagtcacatg actcgtacgg tggcggtagg ggtgcgccct acccatggag
     1201 accggacgaa aacgcttcat cactggcccc tgatccgccg tagctgctac tatacgcagt
     1261 ttgtcggtgt ccatgccgaa gttgtgttcc gcattggcca ctgcgctctg caacactttg
     1321 cttacgagtt tcgccgactt ctgcgggctg tagcggagag cgacaagcgc ttctccaact
     1381 ttcttgccgc ggataaggca gagcacccgc cgtactttta tgggagaaac ccgtacctgt
     1441 ctggcaacgg cttttgcttc catcaccatt acctcctcac cgaacccttg aggaacgctc
     1501 ctgacccgcg tgtcctccga acttgcgggt tggggcgaat tcgccaagct tatgtccgac
     1561 catattttcg ctgatgtaga tgggtatatg gatccgtccg ttgtgcaccg cgatcgtatg
     1621 gccaatcatg gcgggaacga tcgtcgatct tcgggaccag gtcttcatga ccttcttcgc
     1681 tgttccttcg ttcatatctt cgatcttccg gagcagcttc tgatccacat agggcccctt
     1741 ctttctggaa cgagccattc agtgtccctc cttacttctc gtaccggcgc cgaacgatca
     1801 tcttatcgga cggcttcttc ttccgggtgc ggtatccttt tgcgggcgtt ccccaagggg
     1861 agacgggatg cttgtgcgac ttgctcttgc cttcgccgcc gcccatgggg tggtccacag
     1921 ggttcatcac cataccgcga accattggac gtcttccaag ccaacgggtc ttcccggcct
     1981 tgccgaagga cacgttctca aagtcttcgt taccgacctg gcctatcgtc gccatgcact
     2041 cctgaaggat gaggcggagc tcaccgctgg gcatacgcag gtacgcgtac ttgccttcct
     2101 tcgccatgag ctgtgccgaa gttccggcgg cacggacgag tttcgctcct ctgccgggtt
     2161 cgagctcaag gttgtgcacg gtggttccca ccgggatgtc tttcagcttc agcgcgttgc
     2221 ccgggcggat gtccgactcg gggcccgcga cgatcttgtc gccgacgttc agatccttgg
     2281 gggcgatgat atatcgttta tcgccgtcaa ggtagtgaat gagcgcgatc ctcgcgttcc
     2341 tgttcggatc gtactcgatc gcggcaacct tcccgggaac gttgagcttg ttcctcttga
     2401 agtcgatgac tctgtagagt ctcttcgcgc cgccgcctct gtggcgcatg gtgattctgc
     2461 cgcgattgtt cctgccgccc ttcttgtgaa gaggctcgag aagagagcgc tccggatcct
     2521 ttttggtaag atcgtcgttc ttctgaacgg tcatgaacct gcggcctgga gtataggggt
     2581 tgaacttctt gatcgccatt ggtttccttt ctccttcccg ctaaatgctc gcgccttcga
     2641 agaagtcgat acgctctccc ggagcgaggc tgactatcgc ttttttccat gaacgggtct
     2701 ttccgataaa agcgcccatt ctcttcggct tcggatggat attcaaaata tgaacggccg
     2761 ttactttgac cttgaagatc tcctcaatcg ccttgcgaac ctggatcttg ttcgcggaca
     2821 aatggacttc aaagctgtac ttgttcagct ccataaggcg gctgctcttt tcggtgatca
     2881 gcggacggat gatgatgtcg tgcgctacga ggttcatccg acatacacct cctcaagctt
     2941 ttccacgact tccggggtca ggatcagcgt cccggcgttg agaagatcgt agacattgat
     3001 gttcaccatg ttgatcacct tcgcgcccgg tacgttgcgg gcggacttca atacggcttc
     3061 gttcgcctcg ggaagaatga tgagcggttt cctggctttg accgccgtga ggaacgcaag
     3121 catctccttc gtggagggct tttcgatgtc gaacgattta acgaccgtca taagctcttc
     3181 gcggactttc agcgacagcg cgcttttgat cgcgagacgg cgtactttct tgttcacctt
     3241 ctggctgtaa tccctgggct tgggggcgtg cgtaaccccg ccgccggccc acaggggcga
     3301 acggatgctc ccgtgacggg cgcgtcctgt cttcttctgt ctccagggct tcttgccgcc
     3361 gccccggact tctccccgcg tcttcgcgga ctgcgttccg agtctcgcgt tcgcaagctg
     3421 ggcgacgact acctggtgca tggcgggtac gtgcacgggg gcggcgaaaa cgccgtccga
     3481 aagttgcact tcccctaccg tttctccctg gaagctgacg agtttcaggg taggcatgtt
     3541 atttccggcc tccttctctg ccgactaccc cttcttgaaa agggtcacca ggctgttttt
     3601 cgcgcccggt accgcaccct tgacaagaag caggtgattg tcggagtcca ctgcaacgac
     3661 gacgagattc ttgactgtaa ccttctcgcc gcccattcta ccgggcatct ttttgccctt
     3721 gaaaactctt ccggggtagc tgcttgcgcc gctggagccg ccgcggcggt gcaccacgga
     3781 cgcgccgtgg cttgcgttcg aaccggcgaa atgatacttc ttcacgacgc ccgcgaagcc
     3841 cttacctttg ctcgtaccgg agacgttgat cgtctcgccc tctgtgaaga gagtgacgtc
     3901 gatctgctgg ccgacggcgt actcgtcgac agagtcgacc cggaactcgc gaagcgtctt
     3961 cttcggctcc acttcagcct tctcgaacag acccttgcgg gacttgttca gctttctcgg
     4021 attgggctcg ccaaatccaa ggacgagtgc gctgtatccg tccttcgagg gcgtcttcaa
     4081 atccaccacg gggcatggtc cggcctgtat aatcgtcaca ggcgtggctt ggccctgctc
     4141 gttgtaaatc tgggccattc cgagtttttt ccccagaata ccgatgctca ttacactcca
     4201 actcctttca gctcagagct tcccctacaa tttaatctgg atatccacac cagaaggaag
     4261 gttcagctgc ata
//
LOCUS       DAOPKU010000227         4272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_22651_length_4272_cov_4.525255, whole genome shotgun sequence.
ACCESSION   DAOPKU010000227 DAOPKU010000000
VERSION     DAOPKU010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22651_length_4272_cov_4.525255"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..849
                     /locus_tag="PK849_08025"
     CDS             154..849
                     /locus_tag="PK849_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPE66111.1"
                     /translation="MSVSSRIEDYMETIFAIEISGREATVTDLANTLGVAKATVVAAV
                     RRLVAASLATHERYGTLALTDEGRRRALDIYRRHEHLTFLFHDILGFDPDRAMSIACA
                     MEHELDEKSEGRILGFVDYLVKARREGRSWVKDLLTAMGDERRLPRPLSMLPDGGRGV
                     VTRVTAGGLLRQRLLEMGFVPGTEVCHCSTAPLGDPLVIHVRGCEVALRKTEAVSVWI
                     CPVTGEDSAGASC"
     gene            872..1096
                     /locus_tag="PK849_08030"
     CDS             872..1096
                     /locus_tag="PK849_08030"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HPE66112.1"
                     /translation="MCPITIMSEGTEVTVARVAGGGESSRRLSEMGLIPGAKVRIVQN
                     GGGPLLLKVGDSRFAIGQGMAHKVFVDGSC"
     gene            1179..1469
                     /locus_tag="PK849_08035"
     CDS             1179..1469
                     /locus_tag="PK849_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPE66113.1"
                     /translation="MGLTCSCSGGNERVLSALEIGEGGVVSKIAGDGSLKRRILEMGL
                     VPGTEIRVERKAPFNDPVSVWFRGYELSLRVDEANAVLVRPAGCAGCSGGSR"
     gene            1466..3496
                     /gene="feoB"
                     /locus_tag="PK849_08040"
     CDS             1466..3496
                     /gene="feoB"
                     /locus_tag="PK849_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPE66114.1"
                     /translation="MSWTIALAGNPNTGKTSLFNALTGSNQHVGNWPGVTVERKEGRF
                     RSSGDEVTVVDLPGIYSLGAASLDEEIAADYLLRNRPDLAVVVADASNLERSLYLAVQ
                     MLEIGTPLVLALNMMDMAAERGIMIDVERLSALLGIPVVPTVARKKEGIEELKERITR
                     SLRENSAPSLSFALPYGERLNPLFEALETFLGERQELIPGLSARSAAVKFVEGDVAVL
                     AAAESSGVRNSMDELLAEKGRKVEEELGYDLQTAVIERRWGFVSGVTAESVQRDLSLK
                     ARLTLSDRIDRVVTSRSLGLPLFFLVTWAIFRMTYALGDPLVELLEGVFESFGEWTGE
                     FFAGAGFSEIVSSFVQDGLIGGVGSVVVFFPHIFILFAFIALLEDSGYMARGAFVMDR
                     VMHLMGLHGKSFIPMLMGFGCNVPSMMATRILEQPRDRMITLLVLPFMSCSARLPVFV
                     LFSGIFFGAYAGTAVFSLYVLGIVVAVLAAKVLGSTLFKGESSQLVMELPPYHVPSFS
                     MVLRHAWERGFLFLQKAGTFILLSVIAVWVLASFPWGVEYGSSESIVGRIGQILAPVF
                     APAGFGFWQAAVALFFGFLAKEVVVGTLGALLGAGEAGLAAALPGLFTPLSAYAFLVM
                     TLLYVPCVAAVAAFRRETGSWAWTFFMIAYTTLIGYGGAVLVYQGGRMLGLG"
     gene            3664..>4272
                     /locus_tag="PK849_08045"
     CDS             3664..>4272
                     /locus_tag="PK849_08045"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPE66115.1"
                     /translation="MPFSFSSISLERADEYRQLYSQSPRKSAWYSFGSLWAWRNIYGF
                     EWAFEEGVCWIRTRNDALWAPVGPWGAIEWKKVLPRLFPEGRVFFYVPDGLGRLWREL
                     FRGGVTVGEDRSQWEYLHSVQELVELKGNRFSRKRSHFVQFVRRYPFRYRSLEGDALV
                     SVLEGQELWMKEQSVSPDLEREDAAIRELVAQRGHIPGLLAGI"
BASE COUNT          797 a         1064 c         1386 g         1025 t
ORIGIN      
        1 cggacgaatt gctccgggat atgcgtttcg aaaaacgcat tcgttggttg tccttgaatc
       61 ggtgaacaga gtgcttgaca gaaagtgaca cgcgaagtaa gatatatcta accaaaagta
      121 agcgttctct tacatccgaa aggagaagat accgtgtcgg tttcgtcccg aatcgaggac
      181 tatatggaga caatcttcgc catagagatt tccggacggg aagctacggt taccgacctc
      241 gcgaatacgc tcggcgtggc caaagcaaca gttgttgcgg cggttcgcag gcttgtggcg
      301 gcgtctcttg cgacgcacga acgatacggt actctcgcgt tgacggatga gggcaggcgg
      361 cgagcgctcg atatctatag gcgccacgag catcttacct tcctttttca cgatatcctc
      421 ggcttcgacc cggaccgtgc gatgtccata gcatgtgcaa tggaacatga attggacgaa
      481 aagtcggagg gacggattct cggatttgtc gattaccttg taaaggcccg cagagagggg
      541 cgttcctggg taaaggacct tctcacagcg atgggagatg agcgaaggct tccaaggccg
      601 ctttcaatgc ttcccgacgg aggacggggt gttgtgactc gcgtgactgc gggaggtttg
      661 cttcgccaac gccttttgga aatggggttt gttcccggaa cggaagtgtg tcactgcagt
      721 acggcgccgc ttggcgatcc gcttgtgata cacgtacgcg gatgcgaagt ggcgttgcga
      781 aagacggagg ctgtctctgt atggatctgt cctgtaactg gagaggattc agcgggagct
      841 tcctgctgaa cgaagagagg agatgggcag tatgtgtccg ataacgatca tgtccgaggg
      901 aactgaagta accgtagctc gagtggcggg cggcggagag tcttcgcggc gcctttccga
      961 gatggggctt atccccggag cgaaagtgcg gatagtccaa aacggaggag ggcctcttct
     1021 tttaaaggtg ggcgattcgc gttttgcgat cggtcagggg atggcgcaca aggtctttgt
     1081 ggacggaagc tgttaggcgg agcgctcaag gaccggcagg tccttttttc agattccgcg
     1141 ttgagttaga gttagtgaac aaagaacgga gggtcattat ggggttgacg tgtagctgtt
     1201 ccgggggaaa tgagcgtgtg ttgagcgctc ttgagatagg agagggcggc gtcgtatcca
     1261 aaatcgccgg agacgggagc ttgaagcgga ggattcttga aatggggctg gttccgggga
     1321 cggagatacg cgtcgaaagg aaagcgcctt ttaacgatcc tgtctccgtg tggttcagag
     1381 gctatgaact gagtctcaga gtcgacgagg cgaatgcggt cctagtgcgc cccgcaggct
     1441 gcgcgggctg ctcggggggg tcccgatgag ctggacgatc gcgctcgcag ggaacccgaa
     1501 tacgggaaag acgagtctct tcaatgcgct caccggttcc aatcagcacg tggggaactg
     1561 gcccggcgtg actgtcgaac gaaaagaggg gcgattccgt tcctcgggag acgaggtgac
     1621 tgtggtcgat cttcccggca tttacagcct gggggcggcg tcgctcgacg aggagatcgc
     1681 ggcggactat ctgttgcgga accggcccga cctggctgtt gtcgtggcgg atgcgtccaa
     1741 cctggaacgc agcctctatc ttgccgtgca gatgcttgag atcggcactc cgctcgtcct
     1801 tgcgctgaac atgatggaca tggcggcgga gaggggaatt atgatcgacg tggaacgcct
     1861 ttcggcgctt cttggtattc ccgtcgtgcc gaccgtcgca cggaagaagg aaggaataga
     1921 agaactcaaa gagagaatta ctcgttctct tcgggagaac tcggcgccct ctctttcttt
     1981 cgccctgccg tacggagaac gcctgaatcc cttgttcgaa gcgcttgaaa cgtttctggg
     2041 ggagcgccag gaactcattc cggggctttc cgcccgttcc gcggcggtga agttcgtcga
     2101 gggcgacgtt gcggttcttg cggccgcgga gagcagcggc gttcgcaaca gcatggacga
     2161 actccttgcc gagaaggggc gtaaggtgga ggaggagttg ggatacgatc tccaaactgc
     2221 cgtgatcgaa agaaggtggg gctttgtttc gggcgtgacg gcggaatcgg tacaacgcga
     2281 cctctcgctc aaagcgcgcc tgaccctttc ggatcgcatc gaccgcgtgg tgacttcgcg
     2341 atctctaggg ttgccgctct tctttctcgt cacatgggca atattccgca tgacctacgc
     2401 cttaggcgac ccgttggtgg agctcctgga aggagtgttc gaatccttcg gggaatggac
     2461 gggcgagttt ttcgccggcg ccgggttttc ggagatcgtc tcatccttcg tacaggacgg
     2521 gctcattggg ggcgtcggct cggtcgtcgt cttcttccct catatcttca tccttttcgc
     2581 gttcatcgca ttgctggagg attcggggta tatggcgcgg ggtgcgtttg tgatggacag
     2641 ggttatgcac ctgatggggc tgcacggcaa gagtttcatc cccatgctca tgggttttgg
     2701 gtgcaacgtg ccttcgatga tggcgacgcg tattcttgaa cagcccaggg acagaatgat
     2761 cacgctgctt gtgctgcctt ttatgagctg ctcggctcgt cttcctgtct ttgtgctctt
     2821 ttcgggaatt ttcttcggtg cgtatgcggg aacggccgtc ttttccctgt atgtcctcgg
     2881 aatcgtcgtg gcggtgctcg cggccaaggt gctgggcagc acgctcttta aaggggagtc
     2941 gtcgcagctg gtcatggagc ttcccccgta tcatgttccc tcgttctcga tggtgctgcg
     3001 tcatgcttgg gagcgtggat ttctctttct ccagaaagcg gggacgttca tccttctttc
     3061 ggtgatcgcc gtatgggtcc tcgcaagctt cccgtggggc gtggagtacg gttcgtccga
     3121 gagtatcgtc gggcgtatcg gccagatact cgccccggtt ttcgctcccg cgggctttgg
     3181 tttctggcag gctgctgtgg cgctcttctt cggtttcctc gcaaaagagg tcgtcgtcgg
     3241 aacgctgggg gcgcttctcg gagcagggga ggcggggctt gccgcggcgc tgccgggcct
     3301 cttcacgccg ctctccgcgt atgccttcct cgtgatgacg ctcctgtacg tgccgtgcgt
     3361 ggccgctgtc gccgccttcc gcagggagac cggaagctgg gcctggacat tcttcatgat
     3421 cgcctatacg acgctgatag gctacggggg cgcggtactg gtctatcagg gcgggagaat
     3481 gctgggtctg gggtaagtat ttcccccatc ggcattttta ctgaaaaaaa ctgcggcggc
     3541 cccccttttc agaagaagcg ggggtccgcc gcgtttcaat atggtacaat acgcgatgtc
     3601 tgaaaaaggg ttccggagtg tctccggaat tctttggcgt agcagtgaaa ggaagatgtt
     3661 ttcatgccgt tttctttttc atcgatctcc ctggagagag cggatgaata cagacagctc
     3721 tactcgcagt ctccgcggaa atccgcgtgg tattctttcg gaagtctctg ggcgtggcga
     3781 aacatctatg gctttgaatg ggccttcgaa gaaggcgtct gctggatccg gacacggaac
     3841 gacgcgctgt gggctcctgt gggaccgtgg ggcgctattg aatggaaaaa ggtgcttccc
     3901 cgtctttttc ctgagggaag agtctttttt tatgttcccg acggtctggg gcgtctctgg
     3961 agagaactct tccggggagg agtgaccgta ggagaggacc gctcccagtg ggagtacctc
     4021 cactctgttc aggagctcgt cgagttgaaa gggaaccgtt tttcccgaaa acgaagtcat
     4081 tttgtccagt ttgtccggag gtatcctttc cgttatcgtt ccctagaggg agatgctctc
     4141 gtgtcggttc ttgaaggtca ggaactctgg atgaaagagc agtccgtgtc tccggatctt
     4201 gagcgggaag atgcagcgat tcgcgaactt gttgcccagc gcggacatat ccccgggctt
     4261 cttgccggaa ta
//
LOCUS       DAOPKU010000228         4254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_22769_length_4254_cov_4.819719, whole genome shotgun sequence.
ACCESSION   DAOPKU010000228 DAOPKU010000000
VERSION     DAOPKU010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22769_length_4254_cov_4.819719"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PK849_08050"
     CDS             <1..1095
                     /locus_tag="PK849_08050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPE66116.1"
                     /translation="CVPGPFGSGKTVVQHQLAKWAEAEIVVYVGCGERGNEMTDVLLE
                     FPELEDPRSGHPLMKRTVLIANTSNMPVAAREASIYTGITMAEYYRDMGYSVALMADS
                     TSRWAEALREMSGRLEEMPGEEGYPAYLGTRLASFYERAGRAIVLGGDKREASVSAIG
                     AVSPPGGDLSEPVSQNTLRVAKVFWGLDATLAYQRHFPAINWLLSYSLYTEKLDEFWD
                     VKFDDEWSPTRIEAMTLLEDEDKLREIVRLVGVDALSKEERMVLETAKSLREDFLHQN
                     AFHEIDTYASMEKQFKMLKTILAFHHISMDALKKGASMNEVFNLPVREAIARMRYLEE
                     KDIGKIDDIEGQIKNEVAGVTPVGGESDAA"
     gene            1085..2503
                     /locus_tag="PK849_08055"
     CDS             1085..2503
                     /locus_tag="PK849_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPE66117.1"
                     /translation="MLPKEYKTISELSGPLLLVNKTKNVRYDELVEIELASGERRRGR
                     VLEITSDKALVQVFEGTDGIDIKSTKMLFLGKVLTLPVSKDMLGRVFNGRGEPIDDGA
                     PILAEKKLDINGMPMNPFSRDYPSEFIQTGISTIDGMNPMVRGQKLPIFSGSGLPHNR
                     MAAQIARQATVISGHEDFAVVFAAMGITFEEASFFMEDFRKTGAIQRTVMFVNLADDP
                     AIERITTPRLALTAAEYLAFEQDMHVVVILTDLTNYCEALREISAARKEVPGRRGYPG
                     YLYTDLATMYERAGRLKGKKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILSRTL
                     HRKGIYPPVDVMPSLSRLKDKGIGAGKTREDHADLMNQLFAGYARGKEAKELAVILGE
                     GALTEEDKAFAKFADMFEDQYVRQGEYRNRTYEETLELGWDLLTVIPVKELKRVRDAY
                     IAKYLQPRLKSEEKAQAVSGKV"
     gene            2516..3139
                     /locus_tag="PK849_08060"
     CDS             2516..3139
                     /locus_tag="PK849_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPE66118.1"
                     /translation="MARMNVNPNRMELSRLKKRLVVAKRGHKLLKDKQDALIKAFLER
                     ARQGKTLREELEAELAACYKSFLLARAQTLPAMLEQALMISGAKCDLQVGFQNVMSVL
                     VPVYRVEQGGSPLNYGLATSLGSLDVALERFAKLIPKLIELAAKEKAIRLMAVEIEKT
                     RRRVNALEHVLIPGFIETIRYITMKLDEQERSTLSRLMKIKEIVRSH"
     gene            complement(3216..4022)
                     /locus_tag="PK849_08065"
     CDS             complement(3216..4022)
                     /locus_tag="PK849_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HPE66119.1"
                     /translation="MIEKTSYDSPEYDVFKSNVKKLTGLDLNSYKNQIHRRVHMLMQR
                     WNVSSYDIFLKTIKENDQKLREFLDYLTINVSEFFRNPNKWTELQDVVIPRLIQSRGK
                     KQLKLWSAGSATGEEPYSLAILSMEAKLAPQTPVLASDIDQGAIAIAKRGHYLKRQLI
                     NIPKELVGRYFTTNDGGETYLVNSEVKSRVSFQRLNLIEDTFADDLDLILCRNVVIYF
                     CADTKTALYHKFFKALRPGGYLLVGSTEQIFDYRKIGFESAGAFLYQKPA"
BASE COUNT         1090 a         1074 c         1113 g          977 t
ORIGIN      
        1 tgcgtacccg gtccgttcgg ttctggaaag accgttgtcc agcaccagct cgcgaaatgg
       61 gcggaggcgg agattgtcgt ctatgtcggc tgcggagaac gcggaaacga aatgacagac
      121 gtgctccttg aattccccga actggaagac ccgcgttcag gacatccgct catgaaacgg
      181 actgtgttga tcgccaatac ttcaaacatg cctgtcgcag cacgcgaagc aagtatctac
      241 acaggaataa caatggccga gtactaccgt gatatggggt attccgtcgc cctcatggca
      301 gattcaacga gccgttgggc tgaggccctc cgcgagatgt cgggacgtct tgaagaaatg
      361 cccggcgaag aggggtatcc ggcatacctc ggtacccgac tggcctcctt ctacgaaaga
      421 gcgggcaggg cgatcgtcct gggcggagac aagagagagg catccgtcag tgcaataggc
      481 gcagtttcac ctccgggagg agacctttcc gaaccggtaa gtcagaatac gcttcgcgta
      541 gcaaaggtat tctggggatt ggatgcgacg cttgcttacc aacgccactt cccggcgatc
      601 aactggcttt tgagctattc gttgtacacg gaaaaactcg atgaattctg ggatgtcaaa
      661 ttcgacgatg aatggagtcc cacccgtatc gaagccatga cgcttcttga ggatgaagac
      721 aaactgcgtg aaattgtgcg ccttgtcggc gtcgacgcac tctctaaaga ggaacggatg
      781 gtactggaaa cggcaaaatc gctccgagag gacttccttc atcaaaacgc attccacgag
      841 attgacacgt acgcttccat ggaaaagcag tttaagatgc tgaagacaat tcttgcattc
      901 catcatatct cgatggatgc cctcaaaaag ggcgcctcta tgaatgaagt tttcaatctt
      961 cctgtcaggg aggctatcgc ccgcatgcgt tatcttgaag agaaggacat cgggaagatc
     1021 gacgacatcg aaggccaaat taagaacgag gtcgccggag tcactcccgt tggaggtgaa
     1081 agcgatgctg cctaaggagt ataaaaccat cagcgaactt tcgggtccgc ttctgctggt
     1141 aaataaaacg aaaaacgtcc gttacgacga gttggtagaa atagaactcg caagcggtga
     1201 acgccgccgt ggaagagttc tggagatcac ctcggacaag gcgcttgtgc aggtgtttga
     1261 aggaaccgac ggcatcgata ttaaaagcac caagatgctc tttcttggca aggtgctcac
     1321 tcttcccgta agcaaggata tgctcggcag agtctttaat ggccgcggcg agcctattga
     1381 cgatggagct cctattctcg cagaaaaaaa gctggatatc aacggtatgc cgatgaaccc
     1441 gttctcaaga gactatcctt ccgagttcat tcagacgggt atctcgacga tcgacggaat
     1501 gaatccaatg gttcgcggcc agaagctgcc gattttctcc ggcagcggcc taccgcataa
     1561 cagaatggcc gcgcagatcg cccgtcaagc cactgtcatc agcggacacg aggacttcgc
     1621 ggtcgtcttc gccgcgatgg gaatcacatt tgaggaagct tcattcttca tggaggactt
     1681 caggaagaca ggagccattc aacggacggt tatgttcgtc aacctggccg acgaccccgc
     1741 gatcgagcgt atcacaacgc ctagacttgc gcttaccgcc gcggaatacc ttgcttttga
     1801 gcaggacatg cacgtcgtgg tcatcttgac cgaccttact aactactgcg aagcgttgcg
     1861 cgagatctct gcggcgcgca aggaagtccc cggccgcaga ggctatcccg gctacctcta
     1921 caccgacctt gccacaatgt acgagcgcgc cggacgtctg aaggggaaaa agggttccat
     1981 cacgcagatt cccatcctca ccatgcccga ggacgacaag acgcatccaa tacccgactt
     2041 gaccggatac atcaccgaag gtcagatcat cctcagccgt actctccaca gaaaaggtat
     2101 ctatcccccc gtggacgtta tgccttctct gtcccgactg aaagataagg gaatcggcgc
     2161 cggcaagacg cgggaagatc acgcggacct catgaaccag ctctttgccg gatatgcgcg
     2221 cgggaaagaa gcgaaggaac ttgcagtcat ccttggcgaa ggcgctctta cagaagagga
     2281 taaggcgttc gcgaaatttg cggacatgtt tgaagaccag tacgtccggc aaggcgagta
     2341 ccggaacagg acttatgaag agactctcga actcggatgg gatcttctga cagtcattcc
     2401 tgtgaaagaa ctgaagcgcg ttcgggatgc gtacatcgcg aagtacctgc agccgaggtt
     2461 gaagagcgag gaaaaggctc aagccgtctc cgggaaagta taaggggggg tcattatggc
     2521 ccgtatgaat gtcaacccca acaggatgga gctctcgagg ctgaagaaac gtctcgtcgt
     2581 ggccaaacga gggcacaaac tcctgaagga taaacaggat gctctgatca aggcgtttct
     2641 cgaaagagcg cggcagggaa aaacgctccg tgaagagctg gaagctgagc ttgctgcatg
     2701 ctacaagagt ttccttcttg cgcgtgctca aacgctgccc gcgatgctgg aacaggctct
     2761 catgatatcg ggggcgaagt gcgaccttca ggttggattc cagaacgtga tgagcgtact
     2821 tgtaccggta tatcgagtag aacaaggcgg ctctccgctg aactacggcc ttgcgacttc
     2881 tcttggcagt ctcgacgttg cgcttgaacg ttttgcgaaa ctgattccga aactcatcga
     2941 acttgctgcc aaagaaaaag ccattcgctt gatggctgtc gagatcgaga aaacacgaag
     3001 acgcgttaac gctctggaac acgttctaat tccaggtttc atcgagacaa tccgttatat
     3061 cacgatgaag cttgacgaac aggagcgctc tacactgagc cgtctgatga aaatcaagga
     3121 aatagtcaga tctcactgac cggtaaagaa aacctgttaa gacacaaaaa aagagagcgg
     3181 aaaccccgct ctcttttttt gtgtcttttc ctttttcacg ctggcttttg ataaagaaaa
     3241 gcccccgcag attcaaagcc gatttttctg taatcaaaaa tctgttccgt ggagcccacc
     3301 aagagataac ctcccgggcg aagagcttta aagaacttgt ggtacaaagc ggttttcgta
     3361 tcggcacaga aatagatgac aacattcctg caaaggataa ggtctaaatc gtcggcgaac
     3421 gtgtcttcta tcagattcaa ccgttggaag gaaactctgg atttcacctc ggagttcacc
     3481 aggtacgtct ccccgccgtc attagtggtg aagtatcgtc ctacaagttc tttcgggatg
     3541 ttgataagct gccgtttaag ataatggccg cgtttcgcta tcgctattgc cccctggtcg
     3601 atatcgcttg cgagaacggg agtctggggt gcaagttttg cctccattga cagaatcgca
     3661 agcgaatatg gctcttcgcc tgttgccgaa cccgcgctcc aaagcttcag ttgtttcttt
     3721 cctcttgatt ggatcagcct tgggattact acatcctgca attccgtcca cttgttcgga
     3781 ttccgaaaaa actccgaaac atttattgta agataatcga ggaactcgcg cagtttctga
     3841 tcattttctt tgatagtttt gaggaaaatg tcgtaggaag acacattcca ccgttgcatc
     3901 agcatgtgta cccggcgatg aatctgattt ttgtaggaat taaggtcaag ccctgtcaat
     3961 ttttttacat tactcttgaa tacatcgtat tctggggaat cgtaactcgt tttctctatc
     4021 attacactct ggctccttcg ctcgataatg cacacccgtt tcgcgcttcg ttctctattg
     4081 tatcgcattt tgtgctcgaa aaaccatttc gttctctttc ggaaaagatg atataatgtg
     4141 cctccgggag tatccttggt ggccgctctt ttttgaagag aggaaagtcc gggctccaca
     4201 gggcaggacg ctgggtaaca cccagttggt gcgaaccaaa ggaaagtgcc acag
//
LOCUS       DAOPKU010000229         4241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_22862_length_4241_cov_4.878882, whole genome shotgun sequence.
ACCESSION   DAOPKU010000229 DAOPKU010000000
VERSION     DAOPKU010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22862_length_4241_cov_4.878882"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PK849_08070"
     CDS             complement(<1..777)
                     /locus_tag="PK849_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPE66120.1"
                     /translation="MTMHNENTFQIENPHALGAQEALEQLRVTPEGLKREDAAGRLRS
                     VGPNRLPEPPKEGVLKRFFKHFHDLLIYILIAAAFITAVLGHFVDTGVILAVVVINAI
                     IGFLQEGKAEEALEGIRKMLSLTAHVRRGGEWEETGAEELVPGDIVRLRSGDRVPADV
                     RLLEMVNLRIEEAALTGESVPSEKDTEAVSSGAGVGDRSCMAYSGTMVTAGRGTGVVT
                     ATGASTEIGKINRMISDVKQLATPLTRQMNRFGKVLSVVVV"
     gene            1087..2127
                     /locus_tag="PK849_08075"
     CDS             1087..2127
                     /locus_tag="PK849_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPE66121.1"
                     /translation="MPVTLKQLAERTGKSITTVSRALAGYDDVSEETKKLVRRVADEL
                     HYTPNFLARSLQKRRSRTISLILPTDSPRFSDPFFSEFLTGVANMAAERGYDLLLSVQ
                     APGEGEMELYQRKVSSGQVDGFVVVRTRRRDPRIAWLYKSQVPFVAFGRTENGADFPF
                     VDVDGVKGMRLIGEHLIGLGHRRIVFLSSPSELLFVEHRLDGLRQAMTTHGLDPDEMR
                     ILQGDLTQKSGYEATASLLDGGNPPSAIVACNDLMALGAMSALHERGISVGPDIAVTG
                     FDDIPMAEHAHPPLTTIHQPIYEIGRMVCEKLVRLIAGEELASRSEILEPRLIVRSSC
                     GSLRKQPDQTGR"
     gene            2261..3568
                     /locus_tag="PK849_08080"
     CDS             2261..3568
                     /locus_tag="PK849_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66122.1"
                     /translation="MRKHVKRTRAFAALLTLSAILWCGAAFAAGLSGLVFFSTQFAPV
                     EEQAKFREILKDGGFDYTADSNDGPLIDLVTAETQAGKGTVGLIGALHGTFPPLQRVN
                     ALMNLIDLAEDLSESREFAPAFMQTGLLGSEDALYYIPWMQATYIMAANKKALQYLPE
                     GADLKTLTWQQLADWSKALHKAEGKPLLGLPHAGLFQRFLEGYLWPSFTGGMVSKFQS
                     KEAVAMLEWVKAELWPHVHPESINYNLMHEPLLKGDVWVAFDHTARLLPAFNEKPDDF
                     VAFPAPSGPAGLGFMPVIVGLGISKAAPDAEAAKQAIDFLTKPETQARVLKEMGFFPA
                     ISGVDTTNLPRGVAIQMEAVNAQANAANAIPALLPVGLGSRGGEINQIFRNAFDRTIL
                     NGEDIGKVLEEEAANLQRLMDETGAPAWPPDPASDGPSKVLPR"
     gene            3708..>4241
                     /locus_tag="PK849_08085"
     CDS             3708..>4241
                     /locus_tag="PK849_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66123.1"
                     /translation="MSIQPLGTPRRRTKVQWAPYLLITPALLYLLVFFAWPMTQAIRL
                     AVWDEQGLLTLRAAPGEQNKAGALEQGAQITVIDRRNVPLAPGEADGGPALLDVWFFV
                     EGETPGGATVQGWAPESRVRVRETDPSGVPLRGTVRPRLGNTAGQHTTLHASPDERSD
                     VVGELASRAEIGIAQRTS"
BASE COUNT          858 a         1390 c         1241 g          752 t
ORIGIN      
        1 aacgacgacg accgagagga ccttgccgaa cctgttcatc tgcctggtaa gcggtgtggc
       61 gagctgcttt acgtcggaga tcattctgtt gatttttcct atctcggtgg aggctcccgt
      121 cgccgtgacc acgccggtcc cccgccccgc agtcaccatc gtgccggaat aggccatgca
      181 ggaccggtcg ccgaccccgg cgccggacga aacggcctcg gtatcctttt cggagggaac
      241 cgactctccg gtaagcgcgg cctcctcgat gcggagattc accatttcca gcagacgcac
      301 atcggcggga actctgtccc ccgagcgcag acggacgata tcgccgggga caagctcttc
      361 agcgcccgtc tcctcccact ctcccccccg ccgcacatgc gcggtaaggg agagcatctt
      421 ccggataccc tcaagggcct cttccgcctt tccctcctgc agaaaaccta tgatagcgtt
      481 gatcaccacc acggccagaa taactccggt gtcgacgaag tgccccaaaa ctgcggtgat
      541 aaaggccgcg gcaatcaaaa tatagatcag caagtcgtga aagtgcttga agaagcgctt
      601 cagcaccccc tccttcggcg gttccggcag cctgttggga ccgaccgaac gcagacgccc
      661 ggcggcgtct tcacgcttca acccctcagg tgtaactcga agctgctcaa gcgcttcctg
      721 cgccccaagc gcgtgcggat tctcaatttg aaatgtattt tcgttgtgca tcgtcatact
      781 gcacccccct ttgtgtttca caatgcattt tggttatttc ttaatgagca gttacaatct
      841 attgttctag gataacttta aatgccgaat atttcaagga taatcgtttc tcgacgctcc
      901 gcacgcggcc gcgcacacgc tcccgtttca ccggaggaca caaagaagcg tcacaaatga
      961 agaggaaaca tgaagaggaa acgcttcttg acatccaaaa cgttttggtt tagagtacac
     1021 ggcacattca aaacgttttg gttcatctat cggaacaaac gtcgacttct cggggaggaa
     1081 ccgactatgc cggtcacact caagcagctc gcagaacgca ccggaaaatc catcactacc
     1141 gtctcgcgcg cactggcggg gtacgacgac gtcagcgagg agacgaaaaa gctggtgcga
     1201 cgcgtggcgg acgagctgca ctacacgccc aacttccttg cccgttccct tcaaaagcgc
     1261 cgctcccgga cgatcagcct cattctcccg actgacagcc cgcgtttctc cgatcctttc
     1321 ttcagcgaat ttctgaccgg cgtcgccaat atggcagcgg aacgcgggta cgatctgctg
     1381 ctctccgtgc aggcgccggg agaaggggag atggagctct accaacgcaa ggtaagcagc
     1441 ggccaggtgg acggcttcgt cgtcgtccgc acccggcgcc gcgacccccg tatcgcatgg
     1501 ctctacaaat cccaagtccc cttcgtcgcc ttcggacgaa ccgaaaacgg cgcggatttc
     1561 cccttcgtcg atgtggacgg cgtcaagggg atgcgtctga tcggagaaca tctcatcggc
     1621 ctcggccacc ggcgcatcgt ctttctgagc tccccctccg aactgctctt tgtcgagcac
     1681 cggctcgacg gtctgcgcca ggcgatgacc acgcacggcc tcgatcccga cgaaatgcgg
     1741 atactccagg gagatctcac ccagaagagc ggatacgagg cgaccgcctc gctgctcgac
     1801 ggcgggaatc ctccctccgc gatcgtagca tgcaacgacc tgatggctct gggcgcgatg
     1861 agcgcgctcc acgagcgcgg gatctccgtc ggtccggaca tcgccgtcac aggtttcgac
     1921 gacatcccga tggcggagca cgcgcaccct ccgctgacca cgattcatca acccatctac
     1981 gaaataggac gaatggtctg cgaaaagctc gtccggctga tcgccggcga ggaattggcc
     2041 tcccgcagcg agatactgga gccgcggctg atcgtacgct cctcctgcgg ctctctccgg
     2101 aagcagccgg accaaacggg gaggtgatgc ggcgcggacc ttcatccaca gaatgacacg
     2161 ctgcaatacg gcggaaagac aacgggctcc cgccgcctgc ccgatcgttc cagggagtgt
     2221 ttttcaggcg tgggaggatg tgtgtacaag gaggaatgaa atgagaaagc acgtgaaaag
     2281 aacacgagcg ttcgccgctc tgctcacgct gagcgcaatc ctgtggtgcg gcgccgcgtt
     2341 cgccgccgga ctctccgggc tggtcttctt ctccactcag ttcgcgccgg tcgaagagca
     2401 ggcgaagttt cgcgagattc tgaaggacgg gggcttcgat tacactgccg attccaacga
     2461 cggtccgctg atcgaccttg tcaccgcgga aacgcaggcg ggcaaaggaa ccgtcggact
     2521 gatcggcgcg ctccacggca cctttccccc tctgcagcgc gtgaacgcgc ttatgaacct
     2581 gatcgacctg gccgaggacc tctccgaaag ccgggaattc gcccccgcct tcatgcagac
     2641 gggcctcctg gggagcgaag acgctctgta ctacattccc tggatgcagg cgacctacat
     2701 catggccgcc aacaaaaagg cgctccaata ccttcccgag ggcgccgatc tgaaaacgct
     2761 gacctggcag cagctcgccg actggagcaa ggcgctccac aaggccgaag gcaaaccgct
     2821 tctcgggctg ccgcacgccg gacttttcca gcgtttcctg gaagggtatc tctggccgtc
     2881 gttcaccggc ggcatggtga gcaagttcca gagcaaggag gccgtcgcaa tgctggagtg
     2941 ggtaaaggcg gagctgtggc cgcacgtcca ccccgaatcc atcaactaca acctgatgca
     3001 tgagccgctg ctgaaagggg acgtctgggt ggcgttcgac cacacggcgc gtctgcttcc
     3061 cgcctttaac gaaaaacccg acgacttcgt cgccttcccg gcgccctccg gtccggccgg
     3121 gctcggattc atgccggtca tcgtggggct cggcatctcg aaggccgctc ccgacgcaga
     3181 ggccgcgaag caggcgatcg acttcctgac gaagcccgag acgcaggcca gagtcctcaa
     3241 agagatgggc ttcttccccg cgatcagcgg cgtggacacg acgaacctcc cccgcggcgt
     3301 cgccatccag atggaggcgg taaacgcgca ggccaacgcc gcgaacgcaa ttccggccct
     3361 gctccccgtc ggcctcggtt cgagaggcgg cgagatcaac cagatcttcc gcaacgcctt
     3421 cgaccggaca atactgaacg gcgaggacat cggcaaggtg ctggaggagg aggccgcgaa
     3481 cctgcagcgc ctgatggacg agaccggagc gcccgcgtgg ccgcccgatc ccgcttccga
     3541 cggcccgagc aaagtcctgc cacgctagag gtacattggg ttccggcgtg aatgccactc
     3601 tcgggggaga gttcgccggg cgcgtgctct ccccgttcac agcgggtatc cgcacgttca
     3661 caaaagcgcg gatactccgc gtcttccgct gaagggagat gaaaaacatg agcatacagc
     3721 ctctcggaac accaaggcgc cgcacgaaag tgcagtgggc gccgtacctc ttgattacgc
     3781 ccgccctgct ctatctgctg gtcttcttcg cctggccgat gacgcaggca attcgcctgg
     3841 cggtctggga cgaacagggc ctcctcacgc tgcgcgcggc gccgggagag cagaataagg
     3901 cgggcgcgct cgaacagggg gcgcagataa ccgtgatcga ccgccggaac gtccctcttg
     3961 cccccggcga agcggacgga ggccccgccc tcctcgacgt ctggtttttt gttgagggcg
     4021 agacccccgg cggagccacg gtccaagggt gggcgcccga aagccgcgtc cgcgtccgcg
     4081 aaactgatcc ctcgggcgtc ccgctccggg gcacggttcg cccccgcctc ggaaacaccg
     4141 ccggacagca cacgacgctg cacgcttccc ccgacgaacg gagcgacgtc gtcggggagc
     4201 ttgcgagcag agcggagatc ggtattgcgc aacggacatc t
//
LOCUS       DAOPKU010000230         4240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_22875_length_4240_cov_4.668100, whole genome shotgun sequence.
ACCESSION   DAOPKU010000230 DAOPKU010000000
VERSION     DAOPKU010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22875_length_4240_cov_4.668100"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1666)
                     /locus_tag="PK849_08090"
     CDS             complement(<1..1666)
                     /locus_tag="PK849_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPE66124.1"
                     /translation="MDSRDDAAKTEEMKLEWEKMFFFCDDPARTRRYFQIIKAVFDNT
                     VEGIVITDANGIIRMVNAAFSTITGYAPEEALGKNPRILKSDRHPPEFYENFWKRLIN
                     DGKWEGEIWNRRKNGEVYPEWMVCSAVYDEEGNAEHYISVFNDLSEVKFKDAQIELRS
                     NYDYLTGLPNKEMFQSRLSGELAHRERNSERLALLVFDINRFKNINDTLGHFVGDSVL
                     RKVGSLLSECIGSGGLVSRIGGDDFHILLPDVHTPDEIVGQVKRVFECLSDPVDVEGH
                     RLFLSASVGISVFPEDGRDVDDLLKRADIAMGKAKESGISSYRFFTEELGRRASLKLT
                     LENALREGLERNEFHLHYQPKYCIRRGAFSGAEALLRWNSPEGPVPPSDFIPLAEETG
                     LILSLGEEVFRKVCRQIAEWRSRGYSPGEIAVNLSARQFHQKGLLSRMKGILGEYDVP
                     PSLLGIEITESGIMENLMDSIAVLSGMKDLGMTVYVDDFGTGYSSLNYLKRLPIDVLK
                     IDKSFIDGVLEDPNDAAITRAVVGIARSLGLRVVAEGVETPEQLEFL"
     gene            complement(1674..2768)
                     /locus_tag="PK849_08095"
     CDS             complement(1674..2768)
                     /locus_tag="PK849_08095"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE66125.1"
                     /translation="MRPVHIVYVTAGNGHRMAARALREAFDREDVPNVVMDLLDFSSD
                     LFKWSYSDVYAFVSEHSHLACRIMYDLTNKNREESIVLRMYEKISLENVKKFMRYIAE
                     NEPEKCICTHFFPLNVLSRMKAAGLYGGDLYTVITDYGLHKMWVNEYVDRYFIPSEMV
                     REGLLESGVPKEKIIMSGIPVLPKFARISEGKRSFSGRRLPVLFIASSIYNTLVVGIL
                     KELERTGLPLDVTVVTGRNHDLADDLERFSFNSKLSLNVLGFVDTLDKLMTKHDVLIT
                     KPGGLTVSEALCASIPMILVNPIPKQETNNSAYLQSKGAGILARTPEDVGGLVRRFYD
                     DRSTLSSMQRAAASIAFPNAASAVVSGVLR"
     gene            complement(2973..>4240)
                     /gene="larA"
                     /locus_tag="PK849_08100"
     CDS             complement(2973..>4240)
                     /gene="larA"
                     /locus_tag="PK849_08100"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPE66126.1"
                     /translation="YGMKTKMKSGKEEIFLSIPDKNVIAVLEPSGGSPVGDLGEEVRR
                     LLAAPTAGPSLEEIVLKGPARSVAVIVNDMTRSTPTASMLPPLLEELERLGVGREKVT
                     VVVATGTHRPMTDDEIRVVLGDAVFGTVKVENHDCDSPDLVDMGILSTGNRLLINKTV
                     AEADLRIAIGEVLLHYYAGFAGGRKSILPGVAGRETVMNNHKMMTEPGVGIGVVDGNR
                     LSEEMVEAVEKFCPLHFILNCVSDSSKHIVRVVGGHWYDAWREGIATFRKYNFAPVSR
                     KADVVFLSAGGYPKDINMYQAHKALYMASRAVRPGGTLVFFAELAEGYGHKVFEEWAR
                     RGMTHDEVIAAFTADFRFGAHKLFYLAQLAKECTILLYSRAGEEDSRLMFCEKCDLTD
                     TLPSLVRKYGEDFTSYVIPQAGIVLPVEE"
BASE COUNT          903 a         1275 c         1169 g          893 t
ORIGIN      
        1 gcagaaattc aagctgttca ggagtctcca ccccttccgc aacaacgcgc agccccagcg
       61 aacgggcaat gccgacgacg gccctggtga tggcggcgtc gttgggatcc tccagaacgc
      121 cgtcgataaa actcttgtct atcttcagta cgtcgatggg aaggcgtttg agatagttca
      181 gcgacgaata cccggtgccg aagtcgtcca cgtacacggt catccccaag tctttcatcc
      241 cggaaagcac agcgatggaa tccatgaggt tctccatgat gccggactcg gtgatctcga
      301 tcccgagcag agagggcggc acatcgtact cgccaagaat acctttcatc ctcgagagaa
      361 gccctttctg atgaaactgg cgggccgaaa gattcacggc gatctcgccc ggagagtagc
      421 cgcgactcct ccattcggct atctggcggc acaccttcct gaagacctct tctcccagag
      481 agaggatgag ccccgtctcc tccgcaagcg gaataaagtc ggagggcggt acggggcctt
      541 ccggactgtt ccagcgcagg agcgcttccg ccccggagaa agccccccgg cgaatgcagt
      601 acttgggctg atagtgaagg tgaaactcat tcctttcgag cccctcgcgc agcgcgttct
      661 ccagggtgag cttcagcgac gcccgtcttc caagctcttc cgtgaagaat cggtagctgc
      721 tgattccgct ctcctttgcc ttccccatcg cgatatccgc ccgtttgagc aggtcgtcga
      781 catccctgcc gtcctcggga aagacgctga tcccgacgct cgcgcttaaa aagagacgat
      841 gcccttcaac atccacgggg tcgctgagac attcgaagac ccgtttgacc tggccgacaa
      901 tttcatccgg ggtatggaca tccgggagca gaatatgaaa atcgtccccg ccgatacggg
      961 agactaatcc gcctgaaccg atgcactcgg agagcagact gccgactttt cgcagtaccg
     1021 aatcgccgac gaaatgaccg agcgtatcgt tgatattctt gaaacggttg atgtcgaaga
     1081 caagcagcgc gagccgctcg ctgtttctct cccggtgggc gagttcgccg gagagacggc
     1141 tctggaacat ctccttgttc ggcagaccgg tgagatagtc gtagttggag cggagctcta
     1201 tctgggcgtc cttgaatttc acctcggaaa ggtcgttgaa tacggagata tagtgctccg
     1261 cattcccctc ctcgtcgtag acggcggagc agaccatcca ctcggggtag acctccccgt
     1321 tctttcttcg gttccatatc tccccctccc acttcccgtc attgatgagc cgcttccaga
     1381 agttttcgta aaactccggg gggtgccggt cggacttcaa aatccggggg ttcttgccaa
     1441 gcgcctcctc cggagcgtat ccggtaatcg tcgaaaaggc cgcgttgacc atccggatta
     1501 tcccgttggc gtccgtgata acgatacctt ctacggtatt gtcgaaaaca gccttgataa
     1561 tctggaaata gcggcgcgtc cgggcaggat cgtcgcagaa gaagaacatc ttctcccact
     1621 ccagcttcat ttcttccgtt tttgcggcat cgtctcggga atccatcggc cggtcacctc
     1681 aacacgcccg aaacgaccgc ggaagcggcg ttcgggaagg cgatggaggc tgcggcgcgc
     1741 tgcatcgagg agagtgttga acgatcgtcg tagaagcgac ggacaagccc gccgacatcc
     1801 tcgggagttc gggcgaggat ccccgctcct ttcgattgaa ggtacgccga gttgtttgtc
     1861 tcctgcttgg ggatggggtt cacaaggatc atcggtatgg aagcgcagag ggcttcgctc
     1921 acggtgagcc cccccggttt ggtgatcagc acgtcgtgct tcgtcataag cttgtccagc
     1981 gtatcgacga atcccaggac attcaatgat agcttggaat tgaatgaaaa gcgttcgaga
     2041 tcgtccgcga gatcgtggtt ccgccccgtc accacggtga cgtcaagagg gagccccgtg
     2101 cgctccagtt ctttaagtat accgacgaca agcgtgttgt aaatgctgct cgctatgaag
     2161 agcaccggga gacgccttcc ggagaacgac cgcttcccct cgctgattcg tgcaaacttg
     2221 gggagcaccg gtattcccga catgatgatc ttctccttgg gaaccccgga ctcaagaagc
     2281 ccctcccgga ccatttcgct cgggataaaa tagcggtcca catactcgtt gacccacatc
     2341 ttatgcaggc cgtagtcggt aataacggta taaagatccc cgccgtaaag cccggccgcc
     2401 ttcatccggg agagaacatt caacggaaag aagtgggtgc agatgcactt ctccggttcg
     2461 ttctccgcaa tgtaccgcat gaatttcttc acgttctcca gagaaatctt ttcgtacatg
     2521 cggagaacga tactctcctc ccggttttta ttcgtgagat cgtacatgat ccggcaggca
     2581 agatgcgagt gctcgctgac aaaagcgtac acatcgctgt acgaccactt gaagaggtcg
     2641 ctcgaaaaat cgagaagatc catgacgacg ttcggcacgt cctcgcggtc gaaagcctcc
     2701 cgaagagcgc gggcagccat acgatggccg ttccccgccg tgacatacac tatatgaacg
     2761 ggacgcactg tttttccctc cgatgtctga gcattcccct cgctgcgcac ttcattcagc
     2821 aacggaaaat accctgtgga ttctctttat tgtaccgtat gaaatgagaa ggaggaacag
     2881 gaggaaaaaa gaaattccgg gttgaaaaga gagagggccg ccaagccgta cacttgaagg
     2941 ccctctctct cgtggaatta cgttgttgtg aactactcct cgacaggcag cacaatgccg
     3001 gcctggggaa tgacgtacga ggtaaagtcc tccccgtact tccgcacgag ggaaggaagc
     3061 gtgtcggtca gatcgcactt ctcgcagaac atgagacggc tgtcctcttc gccggctctg
     3121 gagtacagaa ggattgtgca ctcctttgcc agctgggcga ggtagaagag cttgtgcgcg
     3181 ccgaagcgaa aatccgcagt gaacgcggcg atgacctcgt catgcgtcat tccgcgccgt
     3241 gcccactcct cgaacacctt atgcccgtac ccctcggcaa gttcggcgaa gaagacgagc
     3301 gtcccgccgg gacgaactgc tctcgacgcc atatagagcg ccttgtgcgc ctggtacatg
     3361 ttgatatcct tcgggtaccc tcccgcggag aggaagacca cgtcggcctt cctggagacc
     3421 ggggcgaagt tgtacttccg gaaggtggcg atcccttccc gccacgcgtc gtaccagtgc
     3481 cccccgacga cccggacgat gtgcttggag ctgtccgaga cgcaattcag gatgaagtgg
     3541 agcggacaga acttctccac cgcctccacc atctcttcgc tgaggcgatt gccgtccaca
     3601 acgccgatac cgacgcccgg ctcggtcatc atcttgtggt tgttcatcac cgtctcgcgc
     3661 cctgcaactc cgggaaggat gctcttccga ccgccggcaa atccggcgta gtagtgcagc
     3721 agcacctctc cgatggcgat cctcagatcc gcttccgcaa cggttttatt aatgagcagc
     3781 cggttcccgg tggagaggat tcccatatcc accaggtccg gggagtcgca gtcgtgattt
     3841 tccaccttca ccgtcccgaa gacggcgtcg ccgagaacga cgcgaatctc atcgtcggtc
     3901 atcggccggt gcgtcccggt ggcgacgacg accgtcacct tctcgcgccc gacgccgaga
     3961 cgctcaagct cttcaaggag cggcggaagc atcgaagcag tcggcgtgga acgggtcatg
     4021 tcgttgacaa tcaccgccac ggatcgcgcc ggacctttca ggacaatctc ctcaagagaa
     4081 ggccccgcgg tcggcgcggc aagcagcctg cgcacctctt ccccgagatc cccgaccgga
     4141 ctcccgcccg aaggttcaag gaccgcgatg acgttcttat cggggatgga gagaaatatt
     4201 tcttccttgc ccgatttcat ctttgttttc attccgtaca
//
LOCUS       DAOPKU010000231         4227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_22971_length_4227_cov_4.779722, whole genome shotgun sequence.
ACCESSION   DAOPKU010000231 DAOPKU010000000
VERSION     DAOPKU010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22971_length_4227_cov_4.779722"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PK849_08105"
     CDS             <1..554
                     /locus_tag="PK849_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPE66127.1"
                     /translation="TEEHEQTVGRELLLLPYKIEAILQQQELLYRLAILHSDARDFLF
                     LGRGVSFPVALEGALKLKEISYIHAEAYAAGEMKHGPIALLDPRVPVVVIAPKDDLHE
                     KTFSNILEAKARKAPVIAIASEGDRSLDSAADNVFNVPSTLPMLSPFLTVVPLQLFAF
                     FVAKLLGCDIDQPRNLAKSVTVE"
     gene            complement(614..1708)
                     /locus_tag="PK849_08110"
     CDS             complement(614..1708)
                     /locus_tag="PK849_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66128.1"
                     /translation="MFSTTLRKLRAARLALLLTLFFACSGEAAPILYIVRIPLVLGEE
                     AQAVLPDGKTIPLGKVAALPTSSRWPGYTASKWAPTGSVAASAVNAVHLTLSVEKEKG
                     RTVSILPRHTVAPAAGEQSFIALDSPAGTGFFGGWAPPVATPVLVRRNDGALVPLEER
                     GLPREGDTLIFEVSESESPYLIDIENRPGGRVLGWYESGPRLLARVIRPLKGVGRFGG
                     TEFQNIGRIRANHSGVIDVSTTPRGVVGGFQILPFLHSKSQEMSSAWQLTQWLIIASP
                     TDRPLPGTAPLFSSNLVPGSQMTDVLWDMWSTYGRKPLVLCRRGGGAWQRLPEASGRN
                     DSALGDLTHLRIYSPFTEEPQKGFVPGTGK"
     gene            complement(1738..2244)
                     /gene="mrtS"
                     /locus_tag="PK849_08115"
     CDS             complement(1738..2244)
                     /gene="mrtS"
                     /locus_tag="PK849_08115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM system CAAX-type protease"
                     /protein_id="HPE66129.1"
                     /translation="MTAFIVFAFFLYFPYAWCALRGESPEKYALLWRLSPSSARELLL
                     VSGGALVPLTFISLFFFARYLPPWHVERILPGILSGLAAAVAEETFFRGWLQTLFAEK
                     YSEWKSILFASFFFGLAHIFQSPAAMLAFFPGIIMGLLRSRHGTVFSAILFHWFGNIW
                     SIWFYPHL"
     gene            2436..2509
                     /locus_tag="PK849_08120"
     tRNA            2436..2509
                     /locus_tag="PK849_08120"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2468..2470,aa:Gln,seq:ttg)
     gene            2590..3993
                     /gene="glmU"
                     /locus_tag="PK849_08125"
     CDS             2590..3993
                     /gene="glmU"
                     /locus_tag="PK849_08125"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPE66130.1"
                     /translation="MSASRCVTGALVLAAGKGTRMKSDLPKVLLPVLDQPVLYYILDT
                     LRRCMASSKGCVQQLAVVVGHRGETVQEYLGSNWPEALPLWQHEQLGTGHAVQIAKEW
                     WTGLDHLLVLPGDVPLLTEASLSGLVEEHIVSGADCSFISFHASDPSGYGRVVREGKE
                     ISIVEDKDASEAQKALTEVNSGIYIFKTSSLLPHIYRLDNENAQGEYYLPDIIAMMIR
                     AGGKVTASLSPDEGEFAGINTPLQLADAATALRRRILRRHLLAGVRMQDPDSVWIGPE
                     VVIAGNVLLEPSVQLWGRTEVARGAHVGSFSILRNVVLEEDACVVSHAVLSDSVFRKG
                     SQVGPFIVVRDGAVFEEDSRGGKFVEVKKSRVGKGSKVPHLAYIGDAEIGAETNIGAG
                     TITCNYDGKKKNPTTIGNRCFIGSDTMLVAPVNVGDDGFTAAGSVITQDVPEGALAVG
                     RAKQRNIEGWAKRAGKK"
     gene            4020..>4227
                     /locus_tag="PK849_08130"
     CDS             4020..>4227
                     /locus_tag="PK849_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HPE66131.1"
                     /translation="MGTIGRELKVFSGTAHPAFARRVCSELGIELSRAKHFRFSDGEI
                     GLSIEESVRGADVFVVQPTCNPVNE"
BASE COUNT          938 a         1222 c         1265 g          802 t
ORIGIN      
        1 tcacggaaga gcatgaacag actgtgggca gagagctgct cctcctgccg tacaaaatcg
       61 aagccattct tcagcagcaa gagctgctgt acaggctggc cattcttcac agcgacgcga
      121 gagacttcct cttcctcgga aggggcgtct cctttcctgt ggcgctcgaa ggagcgctca
      181 agctcaagga gatttcgtac atccatgcgg aggcgtacgc tgcgggagag atgaagcacg
      241 gccccatcgc gcttctcgat cctcgtgtgc ctgtcgtcgt cattgcgccg aaggatgatc
      301 tgcatgagaa gaccttttcc aacatcctcg aagcgaaggc gagaaaggcc ccggtgatcg
      361 ccatagcctc cgagggcgac cggagcctcg actcggctgc ggacaacgtc ttcaacgtgc
      421 cgtcgacgtt gccgatgctc tcgccgttcc tcacggtggt gccgctgcag ctcttcgcat
      481 tcttcgtggc gaagcttctt ggctgcgaca tcgaccagcc caggaacctc gcgaaaagcg
      541 tcacagtaga gtaggcggga ctcccgccga agcgcagaaa aaagaacggc agcctgatca
      601 ggccgccgtt cttttacttt cccgtcccgg gcacgaagcc cttctgaggt tcttcagtaa
      661 atggggaata aatacgcagg tgcgtcaggt ctccgagagc ggagtcgttt ctccccgagg
      721 cctccggaag cctctgccac gctcctcccc cccgacggca gaggacgagc ggcttgcgcc
      781 cgtacgtcga ccacatgtcc cagaggacgt ccgtcatctg gcttcccggc acgaggttgg
      841 aggagaaaag cggcgcagtc ccgggaagcg gccggtcggt cggcgacgcg ataatcaacc
      901 actgcgtcag ctgccaggcg gacgacatct cctgggactt cgaatgcaga aacgggagaa
      961 tctgaaaacc tccaacaaca cctcgcggcg tcgtgctgac gtcgataact ccagaatgat
     1021 tcgcccgtat tcttccgata ttctggaact cggtgcctcc gaaacgtccc acgcccttca
     1081 gagggcggat tactcgggcg agcagcctcg ggcctgattc gtaccatccg aggacgcggc
     1141 cgcccggacg gttctcgatg tcgatcaaat acgggctctc cgactccgaa acttcgaaga
     1201 tgagcgtatc cccttcgcgg ggaaggccgc gctcctccaa tggcacgagt gcgccgtcat
     1261 tccgccggac gagaaccggc gtcgccacag gaggagccca gcccccgaag aaacccgttc
     1321 ccgccgggga gtcgagcgcg ataaacgact gctcgcccgc tgcgggagcc acggtatgcc
     1381 gggggagaat gctcacagtc cgccctttct ctttctcgac ggagagcgtc aggtgcactg
     1441 cgttcacagc cgaagcggcg acgctcccgg tgggcgccca tttcgaggca gtgtaccccg
     1501 gccagcggct cgacgtcggc agcgcggcaa cctttccgag cggaatcgtc tttccgtcgg
     1561 gcaaaaccgc ctgcgcctcc tcgccaagaa caagagggat gcgaacgata tagagaatcg
     1621 gcgcggcctc tcccgaacac gcgaagaaga gggtgagaag aagtgcgaga cgcgccgcac
     1681 gcagcttccg gagtgtcgta ctgaacatgg atcatacctc cctttttgta ggcgccgcta
     1741 aagatgcgga taaaaccaga tagaccatat attgccgaac cagtgaaaca aaattgccga
     1801 aaacaccgtt ccgtgcctgc ttcgcaaaag tcccatgatg atgcccggaa agaaggcgag
     1861 catcgccgcc ggactctgga agatgtgcgc aagcccgaaa aaaaaggagg cgaagaggat
     1921 gctcttccac tcggaatact tctcggcaaa cagcgtctga agccagcccc ggaaaaatgt
     1981 ttcctcagcc accgccgccg ccaggccgga gaggatgccc ggaaggattc gttccacatg
     2041 ccagggaggg agatagcggg caaaaaagaa cagcgaaatg aaagtcaacg gaacaagagc
     2101 gccccctgaa acaagaagaa gttcccgggc cgaggaaggc gaaagcctcc acaagagagc
     2161 gtacttttcc ggagattctc cccgcagagc gcaccacgca tacggaaagt aaagaaaaaa
     2221 agcgaacacg atgaatgccg tcatttgaag gctatcatag cacaccgctt ctttatacgc
     2281 acgcacttga gcattctgcg ggattgtttc ttgcgttgca tcgtccggcc ttcacccccc
     2341 ttccaccggg aaaacgcatc tgctgctcat ttgatgcaat ttcgtaagcc ttgacaccaa
     2401 ctcgctttct gttactatat tgagattgtt gaggatgggg tgtagccaag cggcaaggca
     2461 gcggactttg gatccgccat cgctggttcg aatccagcca ccccagccat gtacagtggg
     2521 cagagggggc ctgaagcgga tccccttttt ttgtgttgtg gaaaattcaa tgtaaaggtg
     2581 ttgattcacg tgtccgcttc tcgttgtgtc accggggcgc tggtactcgc cgccggaaag
     2641 gggaccagga tgaaaagcga tcttccgaaa gtcctgcttc cggttctgga tcagccggtg
     2701 ctctattata tcctcgacac gctccggcgg tgcatggcat cctccaaggg gtgtgtgcag
     2761 cagctcgcgg tcgttgtcgg ccacagaggg gagacggttc aggagtatct tggttcgaat
     2821 tggcccgagg cgctcccttt gtggcagcat gagcagctcg ggaccggaca tgccgtacag
     2881 atagccaagg agtggtggac cggcctcgat catctcctcg ttctcccggg agacgtgccg
     2941 cttcttacgg aggccagcct ttccggtctt gtcgaggagc atatcgtttc aggagcggac
     3001 tgttccttca tcagtttcca cgcgtccgat ccctccggct acggaagagt tgtgcgggaa
     3061 ggcaaagaga tctcgatcgt cgaagacaag gacgcctcgg aggcgcaaaa agcgctcaca
     3121 gaggtcaaca gcggtatcta tatcttcaaa acatcctccc ttctacccca tatctaccga
     3181 ctggacaacg agaacgccca aggagaatac tatttacccg acatcatcgc gatgatgatc
     3241 cgcgcgggcg gcaaggtgac cgcctcgctc tccccggacg aaggggaatt tgccgggatc
     3301 aacactccgt tgcagcttgc ggatgcggct acggccctgc gcaggcgtat tctccgccgt
     3361 catctcctgg ccggggttcg tatgcaggat cccgactccg tgtggatagg gcccgaggtc
     3421 gtaatcgccg gcaacgtatt gcttgaaccc tctgtccagc tatggggaag gacggaggtg
     3481 gctcgaggcg cacatgtcgg cagtttttcg attctccgga acgtcgtcct ggaagaggac
     3541 gcctgcgtcg tgagtcatgc ggtgctctcg gacagcgtgt tccggaaagg ctctcaggtc
     3601 ggcccgttta tcgtggttcg cgacggagcg gtcttcgaag aggactcgcg gggcggaaag
     3661 ttcgtcgagg tcaagaagag ccgcgtgggc aaagggagca aagtccccca cctggcgtac
     3721 atcggcgatg cggagatcgg cgcggagacg aatatcggcg ccgggacaat cacgtgcaac
     3781 tacgacggaa aaaagaagaa tcccacgacc atcggcaacc gctgtttcat cggaagcgat
     3841 acgatgcttg tggcgcctgt caacgtcggc gacgacggtt ttaccgccgc gggttcggta
     3901 attacgcagg acgttcccga aggggcgctt gcggtgggca gggcgaaaca gagaaacatc
     3961 gaaggctggg cgaaacgagc aggaaaaaag tgaaatgatt gaaggaggca tccgtgatga
     4021 tgggaaccat cgggcgtgag ttgaaggtct tttccggaac agcccatccc gccttcgcac
     4081 gaagggtatg cagcgagctt ggaattgaac tgtccagggc gaagcacttc cgcttttcgg
     4141 acggagagat agggctctcc attgaggaga gcgttcgtgg cgctgatgtg ttcgtggtgc
     4201 agccgacgtg caatcccgtc aacgaga
//
LOCUS       DAOPKU010000232         4214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23067_length_4214_cov_4.898774, whole genome shotgun sequence.
ACCESSION   DAOPKU010000232 DAOPKU010000000
VERSION     DAOPKU010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23067_length_4214_cov_4.898774"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1137)
                     /locus_tag="PK849_08135"
     CDS             complement(235..1137)
                     /locus_tag="PK849_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPE66132.1"
                     /translation="MEIKRMRTLARRGAARVLSEIRDEWKAFLAVTIGVSIQAVAVVL
                     FVLPNRFPDLGVSGIAVLSNYVFGISPSWVILILNALLMIWAWRELSPRFVMWTMWAV
                     LLFSLLLKLFEFVPVPVIGDKFLAAMVSGVLRGLGAGLVFRVGGSTGGLDIPGVALRK
                     RYGIEMGQFSIYINMGLLGLSFFVVGLDAAIYGAVGLYVFGIVVDNTTRSFDRRKQVF
                     IITNIPEEVSAYINLSLHRGTTRFEGVGGYSKQKRPVLMTLLEPRQVIPLKRFLAEND
                     PKAFMSVSDASEVVGKGFKSWKSL"
     gene            1266..1487
                     /locus_tag="PK849_08140"
     CDS             1266..1487
                     /locus_tag="PK849_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66133.1"
                     /translation="MFIPLEGDGLVSIRRIVALVRHENETAVYLRDGSLLATGFKPET
                     LRKRYNAFCKEARANAEPLRAQRNGGKRT"
     gene            1484..3397
                     /gene="gyrB"
                     /locus_tag="PK849_08145"
     CDS             1484..3397
                     /gene="gyrB"
                     /locus_tag="PK849_08145"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPE66134.1"
                     /translation="MITSPSALQYTAKDIQVLEGLQAVRKRPGMYIGDTSVRGLHHLV
                     YEVVDNSVDEAIGGFCTRIAVTIHENESISVEDNGRGIPTDPHPSNGRPASEVVLTTL
                     HAGGKFDGGAYKVSGGLHGVGVSVVNALSDWLEITICRNGESRSQRFEKGIPATDLSE
                     GIPTEHTGTKVHFLADATIFEEVKYSFDVLGARFREMAFLNPGLSITLDDKREGGRVK
                     EYRYEGGMRSFIEYLNRGKTVLFPDPIVISGEKDGVSVDIGLQYNDGYQERVFGFANL
                     IHTIEGGTHVSGLRTALTRAVNEAARRAKLLKEKEENLSGDDLKEGLTSVISVKLGNP
                     QFEGQTKTKLGNSDVKGIADSIVYEGLVAWFDDHPQIIKTVVEKAIKARQAREAAKRA
                     RELVRKTAMTGLTLPGKLADCSGRNPENTEVYIVEGNSAGGSAKQGRDRSFQAILPLR
                     GKILNVEKARLDKALANAEIRTMIQTLGAGVGDDFDTSKLRYHKVIIMTDADVDGAHI
                     GTLLLTFFYRYMPALIEKGYLYMAQPPLYRVQRGKNVTYCYNEKEMRALLDEAGESAK
                     IVVQRYKGLGEMNPEQLWETTMDPKNRILRKVEIDDEVEVDEYFSILMGDKVEPRRDF
                     ITAHAHEVRNLDI"
     gene            3660..3875
                     /locus_tag="PK849_08150"
     CDS             3660..3875
                     /locus_tag="PK849_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66135.1"
                     /translation="MVEQKKKIQKAQQTSLFAKQLRIVFYTVFICITIVLLAVTLDLV
                     INPAKTWSDSLAIRWISEKRAMFPFFK"
     gene            complement(3947..>4214)
                     /locus_tag="PK849_08155"
     CDS             complement(3947..>4214)
                     /locus_tag="PK849_08155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPE66136.1"
                     /translation="FFEEGGGRLKEGEGIFLFTDGVTEAFDVDESLLSEGLMDRWLGE
                     MRTLGSEALVREMRNRITRFAGGAEQSDDITLLCFRYTGGNKKV"
BASE COUNT          948 a         1306 c         1223 g          737 t
ORIGIN      
        1 gtcccggcgt tcctcctcgg gaaaaagaag ctgccgaaga aaacgcccga ctcgtacgga
       61 ttcaaagacg agagcgaggc cgtggtctac gcgcttgagt tcggcgctcc ttggtcgaca
      121 atccccggag ccaaggagtg gctggcgcag ggagtgtagc agacacaaaa agcggagacc
      181 ggacgcggcg tttcccgtgg tccggcctcc gcttttttgt gaccccttgc cggtctagag
      241 gctcttccag cttttaaacc ccttccctac gacctccgag gcgtcggaga cggacataaa
      301 ggccttgggg tcgttttcgg cgagaaaccg cttcagcgga atgacctggc gcggttcgag
      361 caaagtcatc aagacggggc gcttctgctt ggagtacccg cccacaccct cgaaacgggt
      421 cgtcccccgg tgtagcgaga ggttgatgta cgccgaaact tcctcgggga tgttcgtgat
      481 gataaagacc tgctttctgc ggtcgaacga gcgcgtcgtg ttgtcgacga cgatcccgaa
      541 gacgtacaac cccaccgcgc cgtaaatggc cgcgtccagg cccacgacga agaaggagag
      601 cccgagaagc cccatgttga tgtagatgct gaactgcccc atctcgatac cgtagcgctt
      661 ccgcagggcg acgccgggga tgtcgaggcc tccggtggag ccgcccacgc ggaagacgag
      721 cccagcgccg agaccgcgca gcacgccgga gaccatggcg gcaaggaatt tgtcgccgat
      781 cacgggaacg gggacgaatt caaagagctt cagcagcagc gagaagagga gcaccgccca
      841 catagtccac atcacgaagc gcggcgaaag ctccctccac gcccagatca tcagcagggc
      901 gttcagtatc agtatcaccc aggaggggga gattccgaag acgtagttcg acagcaccgc
      961 gatacccgaa acgccaagat cggggaagcg gttgggcagc acgaagagca ccaccgccac
     1021 cgcctgaatg gagacgccta tcgtgacggc gaggaacgcc ttccactcgt cgcgtatttc
     1081 gctcaaaacc ctcgcggcgc ctctgcgcgc caatgtgcgc attcttttta tttccattgc
     1141 gaccactcct cttgcttctt tcaaattctc cgactccaac ggattcggag cgcatttccg
     1201 cgcactctcc gcggctttct ggtataatag ttcagttaaa gacttgcgaa atggagtgaa
     1261 acaacatgtt catccccctc gaaggagacg gtcttgtctc catacgccgc atagtcgccc
     1321 tggttcgtca tgagaacgaa acggcggtct atttgcgcga cggctcactc ctcgccaccg
     1381 gattcaaacc cgaaacactg aggaaaagat acaacgcttt ctgcaaagag gcaagagcga
     1441 acgccgagcc tcttcgcgcg caacgaaacg gaggaaagag aacatgataa cgagcccttc
     1501 ggctcttcag tacaccgcca aggatatcca ggtccttgaa ggtcttcagg cggttcgcaa
     1561 gcggccggga atgtacatcg gggacacatc cgtccggggg ctccatcacc tggtctacga
     1621 agtggtggac aactcggtgg acgaggcgat cggcggcttc tgtacccgta tcgcagtgac
     1681 catccacgag aacgaaagca tctctgtgga ggacaatggc cgcggcatcc ccacggaccc
     1741 tcatccttca aacgggcgtc cggcttcgga agtggtgctc accacgctcc atgcgggcgg
     1801 caagttcgac ggcggcgcat acaaggtcag cggcggcctc cacggggttg gcgtttcggt
     1861 agtgaacgcc ctctccgact ggcttgagat aacgatatgc cgcaacggag agtcgcgaag
     1921 ccagcgcttc gagaagggca ttcccgcgac ggatctctcc gagggtattc caaccgaaca
     1981 caccgggacc aaggtgcact tcctggccga cgcgacgatc tttgaagagg tgaagtactc
     2041 cttcgacgtg ctcggcgctc gattccggga gatggccttt ttgaacccgg gactctccat
     2101 cacgctcgac gacaagagag agggcggacg ggtcaaagag taccgctacg aagggggaat
     2161 gcgctccttc atcgagtacc tcaaccgcgg caagaccgtg ctcttccccg atccgatagt
     2221 gatttcgggc gagaaggacg gcgtttccgt ggacatcggc cttcagtaca acgacggcta
     2281 ccaggagcgc gtcttcggct tcgccaacct gatccacacg atcgaggggg ggacgcacgt
     2341 ctccggtctc cggacggcgc tcacccgcgc agtgaacgaa gccgctcgcc gggccaagct
     2401 gctgaaggag aaggaagaaa acctctccgg cgacgacctc aaggagggac tgacctccgt
     2461 catctccgtg aagcttggga acccgcagtt cgaggggcag acgaagacga agctgggcaa
     2521 cagcgacgtg aagggaatcg ccgactccat cgtctacgag gggctggtcg cctggttcga
     2581 cgatcatccg cagatcatca agaccgtcgt cgagaaggcc atcaaggcgc ggcaggcccg
     2641 cgaggcggca aaacgcgccc gcgaactggt ccgcaagacc gcgatgacgg gactgacgct
     2701 ccccggtaag ctggcggact gctccggcag aaaccccgaa aacaccgaag tgtacatcgt
     2761 tgagggaaac agcgccggcg gcagcgcaaa gcagggacgc gacaggagct tccaggcgat
     2821 ccttccgctg cgcggaaaaa tcctcaacgt cgagaaggcg cgcctggaca aggcgctggc
     2881 gaacgcggag atccgcacga tgatccagac gctcggcgcc ggcgtggggg acgacttcga
     2941 cacgtcgaag ctgcgctacc acaaggtcat catcatgacc gacgccgacg tcgacggcgc
     3001 gcacatcggc accctgctgc tgaccttctt ctacaggtac atgcccgcgc tgatcgaaaa
     3061 ggggtacctg tacatggcgc agccgccgct ctacagggtg cagcggggga aaaacgtcac
     3121 ctactgctac aacgagaagg agatgcgggc gcttctggac gaggcgggcg aatccgccaa
     3181 gatcgtggtg cagcgctaca aaggcttggg agagatgaac cccgaacagc tctgggagac
     3241 cacgatggac ccgaagaacc gcattctcag gaaggtcgag atcgacgacg aggtcgaggt
     3301 ggacgagtat ttcagcatcc tgatggggga caaggtcgag ccccgcaggg acttcatcac
     3361 cgcccacgcg cacgaggtca ggaacctgga catttagccc gcgaaggaca acgccccgga
     3421 gatgccgctc cggggttttt ctttttcgca cccgccgcgc agggggcggc tccaacgctc
     3481 tccggcacac ggcgccacgc gatgctcccg catatctctt cctcctcgcg gaacatccga
     3541 ggtcgcgttc cgctccgttc tctcatcttc ccctgtacgg ggcaaaagtc ccgatcaaac
     3601 ggaaagaaaa cgtgtataat tcaaagaacg caacagagag acaaaggaga tgatcgcgca
     3661 tggtcgaaca gaagaagaag atccagaaag cgcagcagac atccctcttc gcaaaacagc
     3721 ttcgcatcgt gttctatacc gtctttatct gcattaccat cgttcttctc gcggtgacgc
     3781 tggatcttgt catcaatccc gccaaaacat ggtcggattc tcttgccatc cgatggatca
     3841 gcgaaaaacg ggcgatgttc ccctttttta aataatacac cgacacaacg gaggggccct
     3901 tttcggacgc cctccgttgt gcgcgccatc ctttcagttc ccgctcctat acctttttgt
     3961 tccctcccgt gtagcggaag cagagcagcg taatatcatc ggactgctcc gcccctccgg
     4021 cgaagcgagt aatccgattc cgcatctccc ggacgagcgc ctcgctcccg agcgtccgca
     4081 tctcgccgag ccaccgatcc atgagccctt cgctcaacag cgactcgtcg acatcgaacg
     4141 cctccgtcac gccgtcggtg aagaggaaga ttccctcccc ttccttcagt cgtccgcctc
     4201 cctcttcaaa gaac
//
LOCUS       DAOPKU010000233         4211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23096_length_4211_cov_5.306304, whole genome shotgun sequence.
ACCESSION   DAOPKU010000233 DAOPKU010000000
VERSION     DAOPKU010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23096_length_4211_cov_5.306304"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1849)
                     /locus_tag="PK849_08160"
     CDS             complement(251..1849)
                     /locus_tag="PK849_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPE66137.1"
                     /translation="MAGRIFLTGDVHGDVTSARLGKRLFPEGEGLSKEDFLVVLGDFG
                     LFWHTPRTPEERRCLRSLADRPWTTLFIDGNHENFDLLDALPTEERWGAPVGVAAPGV
                     YHLRRGFVYDVAGLSCFVFGGGRSVDKSVRTPGIDWWERENPGPEERTLGLENLERHG
                     WKVDLVWTHVAPTRACDRLLSDHYAFAHTGRGTAHDPLSDYLDDVAERLSFKLWSFAH
                     YHVSARPFFAGSSGLFTAEYETFREIPIRSGTLPEPKEESAANAEGMDMQLFFFTNKG
                     NVRDANQDALLAGERLVAYEPGKPSHCMERVEAVRSTGNRVLLAVIDGMGGYAGGELA
                     SRIVAESLLDRLPEVISAASAEAAKEYVVRALGTAAELMNELSAEYESLESMGATLAG
                     LVLGKERALLFNVGDCRVYRLRGGVLERVSRDHSEVQRLVDAGVIKEEEMRFHPRKSI
                     VTSAIQAGVGLASTPLFFKEFVPREGDAFFLCSDGVWEALSREEIEKCLLMPDEKAGC
                     EELRERLLDTECRDNVSFVCMRMN"
     gene            2029..2775
                     /locus_tag="PK849_08165"
     CDS             2029..2775
                     /locus_tag="PK849_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPE66138.1"
                     /translation="MRFLSDTPLFDLHNHTLASGHAYGTIRESAEAAADRGLAVLGFS
                     EHAWTVTGAPSRSYFLNFKEIPSRICGVRIVNGIEANILNESGEIDVDEQLGSRLDFV
                     IASLHPSPYYSGPIAKKEIIRAYIETMRRNPFVRIIGHPEDGRFPVDYRSLAEAASDR
                     GVALELNNSGLREKHGRQNGAANAEELLEECARYGAFVVMGSDSHWGGDAGALANCET
                     LIEQTGFPKELVLNYRSDGLAFILRRLLEE"
     gene            2781..3473
                     /locus_tag="PK849_08170"
     CDS             2781..3473
                     /locus_tag="PK849_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPE66139.1"
                     /translation="MEKTHGLFPVLDIDDMTWFSADHHFGHRSVLKFDRRPYADLDEM
                     ERDLVMKHNATVPPNGATVIFLGDVVYPKGDRETDWRDIVRSLHGDRKILLAGNHDAS
                     LVKPYSKVFDAVLPEGVPLTVRLRGREVRCVHSPESLCKRICPGFDDLPSGYADVLLT
                     LEYGNERIEGEWLCGHVHTVFRKLGSIVNVGVDAWDYAPVSVADAFALLDDPRIGIPG
                     RKGFDGDFGETG"
     gene            complement(3517..>4211)
                     /locus_tag="PK849_08175"
     CDS             complement(3517..>4211)
                     /locus_tag="PK849_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66140.1"
                     /translation="ERYWLNWLSEKRWTTLVVDGNHENFDLFDSLPQEERWGGPVGVI
                     APDVYHLKRGYVYEIAGRKVFVFGGGESTDKDGRIPGKSWWPRELPSYAETERGFETL
                     ERAGWKVDWVWTHAAPRRAFDELMENHYAVAHLQREKMDDPVSRYLDELAGRLEFSLW
                     CFGHYHLQSRPFEAGANGLFRAEYREVRPLSVSERMKKALATNDFKDWKIDFDEMIED
                     EGMNPKEPLGGW"
BASE COUNT          822 a         1310 c         1186 g          893 t
ORIGIN      
        1 gatgcaagac gaccttcgtg aatccgagca cctttctgct ccgtcaaccg atgaaaactt
       61 caccgaactc cttccggatc tgaccgaaca aacatccctc taaatttcgc tatccatacc
      121 tatttgtatt tgcattccca aacgccgtgg cttaggctgg ccatgtcttc cattgagttt
      181 ctcttcctca aagtacttta ggcggttcac atcaggctct ttgtgccgca aaatcctttc
      241 gcttttttta ttagttcatc cgcatacaga cgaagctgac gttgtcccgg cactccgtat
      301 ccagcagtct ttcccgtagt tcctcgcacc cagctttttc atcgggcatg aggaggcact
      361 tctctatctc ttcccgcgac aacgcctccc agacgccgtc gctgcacaga aagaaagcgt
      421 ccccctcacg cggaacgaac tccttgaaaa aaagaggggt ggaggcgaga ccgacaccgg
      481 cctggatggc cgaggtcacg atgctcttcc gggggtggaa ccgcatctct tcctctttga
      541 ttactcctgc gtccacaagt ctctgtacct cggagtggtc gcggctcacc cgttcgagaa
      601 cgcctccccg aaggcgatag acgcggcagt cgcccacgtt gaagagaagg gcgcgctcct
      661 ttccgagaac cagtccggca agcgtggctc ccatcgactc gagagactca tactccgccg
      721 agagttcgtt catcagctcc gcagccgtcc cgagcgcccg aacgacgtac tccttggcag
      781 cttccgcaga tgctgctgag ataacctccg gaaggcggtc gagaagggat tccgcgacga
      841 tccgcgaggc gagttctccc ccggcgtatc cgcccatccc gtcgatcacc gcgagaagaa
      901 cgcggtttcc ggtggaacgg accgcctcga cacgttccat gcagtgcgat ggctttcctg
      961 gttcgtacgc taccagccgt tctccggcca gaagagcgtc ctggttggcg tcgcgcacat
     1021 ttcccttgtt ggtgaagaaa aacagctgca tatccatccc ttcagcgttc gcggcgcttt
     1081 cttccttcgg ttcggggagg gtaccggaac ggatcgggat ttcacggaag gtctcgtact
     1141 cggcggtgaa aaggccgctt gaaccggcga agaagggacg cgccgagacg tggtagtgcg
     1201 cgaagctcca gagtttgaag gagagccttt cggcgacatc gtcgaggtag tcggataacg
     1261 gatcatgggc ggttcccctt ccggtgtgcg caaaggcgta gtggtcgctc agcagtctgt
     1321 cgcacgccct cgtcggcgcg acgtgcgtcc agacgaggtc caccttccat ccgtgccgtt
     1381 cgagattctc caatccgagc gttcgctcct ccggaccggg gttctctctt tcccaccagt
     1441 cgattcccgg agtgcggacg ctcttgtcga cgcttctgcc tcctccgaag acgaagcagg
     1501 agaggcctgc cacgtcgtag acgaaccccc ttctgagatg gtagacgccg ggagcggcga
     1561 cgccgaccgg agcgccccat cgctcctccg tcgggagagc gtcgagcagg tcgaagttct
     1621 cgtggttgcc gtcgatgaag agcgtcgtcc acggtctgtc ggcaaggctt ctcaggcagc
     1681 gccgctcctc gggtgtccga ggggtgtgcc agaagagtcc gaagtccccc agcacgacga
     1741 gaaaatcctc tttgctcagc ccctccccct cgggaaagag ccgtttgccg agcctcgccg
     1801 aagtcacgtc accgtgcacg tctccggtca gaaatatccg tccggccatc ctcgccgcct
     1861 ccttttccgt tcttgcagga tcacaactat tgtcgcacgt tcagaacgct aatgaaacct
     1921 tgtacctgaa cgaacctgtt gttagaatga gggacagatc aaggaaacgg ccgccgcgca
     1981 cttcggcgca tgcggccttc aatcgccgga aaggttggtt tgaaaccgat gcgatttctc
     2041 tcggatacgc ccctcttcga cctgcacaac catactctgg ccagcggcca cgcgtacgga
     2101 acaatccgcg agtccgccga agcagccgct gaccggggac tcgccgtgct cggtttctcc
     2161 gaacatgcgt ggactgtcac tggcgctcct tcccgctcgt acttcctcaa cttcaaggag
     2221 attccttccc ggatctgcgg tgtccgcatc gtgaacggaa tcgaagccaa cattttgaac
     2281 gaaagcggag agatcgacgt cgatgagcag cttggatcgc gccttgattt cgttatcgcg
     2341 tcgctccacc cgtctcctta ctattcgggg ccgattgcga aaaaggagat aatccgggcg
     2401 tacatcgaaa cgatgcgccg caaccccttc gtcaggatta tcggacaccc ggaggacgga
     2461 cgtttcccgg tcgattaccg gtcgctcgcc gaagcagctt cggaccgagg cgtcgctctt
     2521 gaactgaaca actccggtct tcgcgagaaa cacggacggc aaaacggcgc cgccaatgct
     2581 gaggaacttc ttgaagagtg tgcgcgctac ggcgcgttcg tggtcatggg gagcgattcg
     2641 cactggggag gcgacgcggg agcgctcgca aactgcgaga cgcttatcga gcagacaggc
     2701 ttcccgaaag agcttgttct caactaccgc agcgacgggc ttgctttcat cttgaggaga
     2761 ctgttggagg agtgacggat atggagaaaa cccacggatt atttcccgtt ctcgatatcg
     2821 acgacatgac atggttcagc gcggaccatc acttcggcca ccggagcgtg ctgaagttcg
     2881 accggcgtcc gtatgcggat ctcgacgaga tggagcgcga ccttgtgatg aagcacaacg
     2941 cgactgtacc tccgaacggc gcgacggtta tcttcctcgg cgacgtcgtc taccccaagg
     3001 gggacaggga gacggactgg agggacattg tccgctctct tcacggagac aggaagattc
     3061 ttctcgcggg caatcacgac gcatccctcg taaagcccta ttccaaagtc ttcgacgccg
     3121 tgctgcccga gggagtccct ttgacggtgc gcctccgcgg gcgggaggta cggtgcgttc
     3181 actcccccga aagcctttgc aagaggatat gccccggttt cgacgacctt ccctccggct
     3241 acgccgacgt tcttctgacg cttgagtacg ggaacgaacg gatcgaaggg gagtggctct
     3301 gcggccacgt acatacggtc ttccggaagc tcggttccat tgtcaacgtc ggagtcgacg
     3361 cgtgggacta cgcccctgtc tccgttgccg acgccttcgc tcttttggat gatccgcgga
     3421 tcggcattcc ggggagaaaa gggttcgacg gggacttcgg agaaacgggt tgagaacagg
     3481 acgtacactt tgaacaagtt ctgcgagtct tctccgctac catcccccga gcggttcttt
     3541 gggattcatt ccctcgtcct caatcatctc atcaaagtcg atcttccagt ccttgaagtc
     3601 gttcgtcgca agcgccttct tcatgcgttc ggatacggag agcgggcgga cttccctgta
     3661 ctccgcgcgg aataggccgt tcgcccccgc ctcgaaagga cgtgactgga gatggtagtg
     3721 gccgaaacac cacaacgaga actccagcct cccggccagc tcgtcaagat agcgcgatac
     3781 ggggtcgtcc atcttctctc tctgcaggtg tgcgacggcg tagtggtttt ccatcaactc
     3841 gtcgaaggct ctgcggggag cggcgtgcgt ccatacccag tcaaccttcc aacctgcccg
     3901 ttcaagggtt tcgaagccgc gctcggtctc ggcgtaggag ggcaactcac ggggccacca
     3961 gctcttgccg gggattcttc cgtccttgtc cgtgctctct cctcctccga agacgaacac
     4021 ctttcttccg gcgatctcgt agacatagcc acgcttcaag tggtagacgt ccggagcgat
     4081 gacgccgacg ggaccgcccc accgctcttc ttgaggcagc gagtcgaaca ggtcgaagtt
     4141 ctcgtggttg ccgtcgacca caagcgtagt ccaccttttc tcagagagcc agttcagcca
     4201 gtagcgctcc g
//
LOCUS       DAOPKU010000234         4206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23142_length_4206_cov_7.004336, whole genome shotgun sequence.
ACCESSION   DAOPKU010000234 DAOPKU010000000
VERSION     DAOPKU010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23142_length_4206_cov_7.004336"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..439
                     /locus_tag="PK849_08180"
     CDS             164..439
                     /locus_tag="PK849_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPE66141.1"
                     /translation="MLVETRRIMPITRLQRELTQTVRDISETGEPVYIVKNNTMEAVM
                     VPFEEYEYLAALEEMFEYYEIDDVVQKRLGTYDPGKNIRWSELSVAA"
     gene            complement(532..1332)
                     /locus_tag="PK849_08185"
     CDS             complement(532..1332)
                     /locus_tag="PK849_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE66142.1"
                     /translation="MIESFFGLHGIPFGRSVPVAELLRTDGWDELHARMLHTARARGF
                     GVFTGDTGTGKTTSLRRFAGELDPNRYRVLYVCDSFLTPRNFYWEVLHQLGCTPRFYR
                     GDAKRQFQKALREVNEERKNPVVVVDEAHLLSREMLEEIRFLLNTGMDSESSLALLLV
                     GQSELKDALKLQVNQAIAGRVDIRFHLGAMDKGQTASYVKRHMDSVKAPRDIFTSTAL
                     GMIHEYAGGIPRKVNKVASSCLMTAAVRKETLVDDHLVQEVLESEFEI"
     gene            complement(1325..2725)
                     /locus_tag="PK849_08190"
     CDS             complement(1325..2725)
                     /locus_tag="PK849_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HPE66143.1"
                     /translation="MKEIELDGDREEGLRRFALVAPLLEEGLSSSEATERRLRILERE
                     EISERTLRRWVAAYRSGGFEGLVKKPRKDKGTCRAISPEALALAEECRRELPRRSAGL
                     IRDWLGTQGHEVARSTLERHLRANGLSGRDLRAEREAKSGSRRFVRIGRNSLWQADLK
                     YGPYIPDPEHPGRKRRTYLLAILDDATRKVTHAEFYDNQKLPVLEDAMKKAIQRHGSP
                     KRIYVDNGKIFVSTWMKLACAKLNIKHINASPYNPEGKGKIERFNRTVEEFLAEYALQ
                     EAGTLEGLNSFFRSWLSERYQHKAHGALNGKTPAEMFAEDATPLRFHSLETLRDAFLH
                     EGERTVDKSGCLKLEGDLYDAGTDYLRKKVTIRYDPFDREIVQLWYKGTRRSDIRKAR
                     IGESNATLKAPAEKLDAGGESRVLNTYREEHRKRFVKTLGAYGLRNAGKSGESRAGEH
                     AAGEERSGSGEEKLRD"
     gene            complement(2783..3274)
                     /locus_tag="PK849_08195"
     CDS             complement(2783..3274)
                     /locus_tag="PK849_08195"
                     /inference="COORDINATES: protein motif:HMM:NF040103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HPE66144.1"
                     /translation="MPESCFFCEGPFRFHGFRKRRCILLGEKRPFFVKRFRCCVCRKT
                     FTVLKKFMLPFKQHAASEIEEALSGEKESGTHADERTIGRWRKAFHTVLPALCALLED
                     LAEAVFGSKVSLSASPESPLKRLRRASETLGTYLPGESALARAMFLESRHRLCLGWQS
                     FRL"
     gene            3475..3792
                     /locus_tag="PK849_08200"
     CDS             3475..3792
                     /locus_tag="PK849_08200"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPE66145.1"
                     /translation="MSFPVEFLPEAADDYKKLDGSVRKDVNKKIDELAENPFLGERLG
                     HKFSIDLTGFLKIYVHGKKYRIVYRLIEPERIEIIEIWGIGKREKEKIYKLIGERVKK
                     RTK"
     gene            complement(3979..>4206)
                     /locus_tag="PK849_08205"
     CDS             complement(3979..>4206)
                     /locus_tag="PK849_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66146.1"
                     /translation="ELTVTQRNILNALEDGTLSRKDIFAQIGITSDTRAFKRHIGPLM
                     SRGFIKMTVPDKPNSKLQKYRLTDSGRAAIR"
BASE COUNT          849 a         1244 c         1143 g          970 t
ORIGIN      
        1 cgcgaacgga tcccgctgaa tctccctgat cagcctgtcg atccgctcga ccatccgctt
       61 gtcctgttta tgctggcgca aacagtcctc tcacgggcgt gaatagtatg tatttgacta
      121 catgctaaag tatgtattat aatcaacatt ggaggtggtc gacatgttgg tggaaacgcg
      181 ccgtataatg ccgataactc gtctgcaaag agaactgact caaaccgtca gagatatttc
      241 cgaaaccggc gaaccggtat acatcgtaaa gaacaacacc atggaggccg taatggtgcc
      301 ttttgaggag tatgagtatt tggcggctct tgaggagatg ttcgagtact atgagataga
      361 cgatgtcgtc caaaagcgtt tgggtactta tgatcctggc aaaaatatac gttggtcgga
      421 attaagtgta gcggcttgat ctccttgtcc ggggtcgact ctttttcgat gaccgcggag
      481 acagctttcc attcgtgtcc gtaaggggcg gaaggatacc gcccctccgg ctcaaatttc
      541 gaattcgctc tccaaaacct cctgcacaag atggtcgtcc acaagagtct ctttcctgac
      601 cgccgcagtc atcagacacg acgacgcgac tttattcacc ttccgcggaa ttcctccggc
      661 gtactcgtgg atcattccaa gcgccgtcga cgtaaaaatg tctctggggg ctttcaccga
      721 gtccatgtgc cgtttgacgt acgaagcggt ctgccctttg tccatggctc ccagatggaa
      781 gcggatatcc acccttcccg ctatggcctg gttcacctgg agtttcagcg cgtccttcag
      841 ttcgctttgt cccaccagca agagcgccag cgagctctcg gagtccattc cggtgttgag
      901 gagaaaacgg atctcctcga gcatttcgcg ggagagcaga tgcgcctcgt ccaccacgac
      961 caccgggttc ttcctctcct cgttcacttc tctcagagcc ttctggaact gccgcttcgc
     1021 gtctccgcga tagaagcgtg gagtgcaacc gagctggtgc agcacctccc agtagaagtt
     1081 gcggggagtc aggaaggaat cgcagacgta cagcacgcgg tatcggttgg ggtccagttc
     1141 gcccgcgaag cgtctgagcg acgtcgtctt ccccgttccc gtatctccgg tgaagacccc
     1201 gaatcctctg gcgcgggcgg tgtgaagcat ccgggcgtgc agttcgtccc agccgtccgt
     1261 ccgcaggagt tcggcgacgg gaacgctgcg gccgaagggg attccatgaa gtccgaagaa
     1321 cgactcaatc acggagcttc tcctctccgc ttccggagcg ctcttctccg gcggcatgtt
     1381 cccctgcgcg gctttccccg cttttcccgg cgttcctcag gccgtacgca ccgagcgtct
     1441 tcacgaaacg cttgcggtgc tcctccctgt aggtgttcag cacacgggat tctcctcccg
     1501 cgtccagctt ctcggcgggt gccttcagcg tcgcgttgct ctctccgatg cgggccttcc
     1561 ggatgtcgct ccgccgcgtt cccttgtacc agagctggac gatctccctg tcgaacgggt
     1621 cgtagcggat cgtcaccttc ttgcgcagat agtccgtccc ggcgtcgtag agatcgcctt
     1681 ccagcttcag gcagccgctt ttgtccaccg tccgctcgcc ctcgtggaga aatgcgtccc
     1741 gcagcgtctc caagctgtgg aatcgaagcg gcgtcgcgtc ctccgcgaac atctccgcgg
     1801 gcgtctttcc gttcaatgca ccgtgcgcct tgtgctggta gcgttccgaa agccaggagc
     1861 ggaagaacga attcagcccc tcgagcgtcc ccgcctcctg aagcgcgtat tccgccagaa
     1921 actcctcgac cgtcctgttg aaacgttcaa ttttgccctt tccctccgga ttgtacggcg
     1981 aagcgttgat atgctttata ttcagcttcg cgcacgccag cttcatccac gtcgagacga
     2041 agatctttcc gttgtcgacg tagatgcgct tcggcgagcc gtgtctctgg atcgccttct
     2101 tcatcgcgtc ctccaacacg ggaagtttct ggttgtcgta gaactccgcg tgcgtcacct
     2161 tcctggtggc gtcgtcgagg atggccagaa gatacgttcg ccgctttctt ccgggatgct
     2221 ccgggtcggg gatgtacggt ccgtatttca aatccgcctg ccacagactg tttcttccga
     2281 tcctgacgaa acgcctgctc cccgatttcg cctccctctc cgcgcgcagg tctcttccgg
     2341 agagtccgtt ggcccgaaga tgccgctcca gcgtcgaccg ggcgacctcg tgcccctgcg
     2401 tacccagcca gtcgcggata agacccgcgc tccgccgggg aagttccctg cggcattcct
     2461 ccgcgagagc gagcgcctcc ggcgatatgg ctctgcacgt ccccttgtcc ttgcgcggct
     2521 tcttcaccag cccttcgaag cccccgctcc ggtacgccgc gacccatctc ctcaacgtcc
     2581 gctccgaaat ctcctcgcgc tcgagaatcc tcagacgccg ctccgtggct tccgaagacg
     2641 acaacccctc ctccagcaac ggcgccacca gagcgaatct ccggagccct tcctctctgt
     2701 cgccgtccag ttcgatttcc ttcatccccg ccacgtcctt cctctccgtt ttttctcctc
     2761 gtccgggagc acggaacgga gtctacaggc ggaaggattg ccatccaagg caaagtcggt
     2821 ggcgggattc gagaaacatc gcccgcgcca gagcggattc accgggaaga tacgtcccca
     2881 gcgtctccga cgcgcggcgc agacgcttca atggagattc cggcgacgcg gacagggaaa
     2941 ccttcgaacc gaacaccgct tccgcgagat cctccaaaag ggcgcaaaga gcgggaagaa
     3001 ccgtgtgaaa agccttcctc caacgtccga tggtgcgctc gtccgcatgc gtcccgctct
     3061 ccttttctcc ggaaagggcc tcttcgatct cggaagcggc atgctgcttg aaggggagca
     3121 tgaacttctt gaggacggtg aacgtcttcc ggcagacgca gcaacggaag cgcttcacga
     3181 agaacggacg cttctctccg agaagaatgc agcgtcgctt gcggaagccg tggaaacgaa
     3241 acgggccttc gcagaaaaaa caggactcgg gcaggggaaa atcttcgttg tcttctggta
     3301 taattcgaat ggtttttcac gatagtcccc cggttcgatc ctgtcttggc ggagaggtcg
     3361 tccgggggac tctcctttct cttcagcctg tgtatacaga ttaccccgga cggactccca
     3421 tgtaaagcct ttaccggtcg aaaagaccgg gccgaaacaa ttaagagaag actgatgtcg
     3481 ttcccggtcg agtttttacc tgaagcggcg gatgattata agaaattgga tggaagcgta
     3541 cgtaaagatg tgaataaaaa aatcgacgag ttggccgaaa acccgttttt aggagagcgg
     3601 ttgggccata agttttcgat cgacttgacg ggcttcctga agatatatgt ccatggcaag
     3661 aaatatagaa ttgtctatcg cctgattgag ccggagagga ttgaaataat agaaatttgg
     3721 ggaattggaa aaagagaaaa agaaaaaatc tataaattga tcggagaaag ggtaaagaag
     3781 cgcacgaagt agggtagggt gatccgtcaa gcggttgttc gccgccatgg ttttcggccc
     3841 gaattcgtac agaccgggta gtcctgcgca tgcaaagagg agagtaatcg gacgaggggc
     3901 tttgaaacac tctcctgagc tgaacagcga acgtcttgat accggaagct tcattggata
     3961 gccgcgttat cgcgcgtgtt accggatagc cgctctaccg ctgtccgtca gacggtattt
     4021 ttgcaacttg ctgttcggct tatccggcac agtcattttt atgaagcctc tcgacatcag
     4081 tggcccgatg tgccgcttga atgcgcgagt atcgctggtt attccgattt gtgcaaaaat
     4141 atctttgcgt gaaagcgtgc cgtcttcaag tgcattcagt atattccttt gcgtcacggt
     4201 cagctc
//
LOCUS       DAOPKU010000235         4204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23165_length_4204_cov_3.619667, whole genome shotgun sequence.
ACCESSION   DAOPKU010000235 DAOPKU010000000
VERSION     DAOPKU010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23165_length_4204_cov_3.619667"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK849_08210"
     CDS             <1..190
                     /locus_tag="PK849_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66147.1"
                     /translation="SLWEWVADSAASFFPWTLSWSPLLATLGGGLFLLTVTGGLFPLR
                     GRTIAGAGILLLAAVFFL"
     gene            319..1086
                     /locus_tag="PK849_08215"
     CDS             319..1086
                     /locus_tag="PK849_08215"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPE66148.1"
                     /translation="MLLVLDVGNTTTVIGIYEGDTLKKHWRLMSERHTSDELGIYLLN
                     LLSISGISLKALEGAIYSSVVPSLDTALSEGLEEYLGVVPVKVTASLDLGMEIAYSPK
                     HEVGADRLVNSVAGKARFGAPLIVVDFGTAITLDILNKEGAYLGGTISPGLVTGMEAL
                     FGRTAKLPQVSLEPPKSVIGNNTIGSIQAGILFGNAGLVDSLVRRTWSELGTKTPVAA
                     TGGHASVVASLSETITHVDPWLTLEGLRLIYERNRAR"
     gene            1083..1856
                     /locus_tag="PK849_08220"
     CDS             1083..1856
                     /locus_tag="PK849_08220"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPE66149.1"
                     /translation="MTLDELPVPPPSVAAHPVEIGGLRLANPLLLAPLAGVTIPALRL
                     LYSKLGAGAVHTEMISCAGLLVSERQSRKRNNTGDEGYEIVSPGSKTERMLAVLEGES
                     PVIAQLFAGDAETMARGAECALGVQSKIVALGINMACPMPKVLKKGAGSKLLERPEVA
                     FDMVRRLVALGLPVWPKIRKCPPTLPLTTEAFCEGLLEAGAASVTVHGRTPAQRYTGE
                     ADVSLLPRLATLFPGKVCASGDVFSPERAAEYLNGGAPQ"
     gene            1853..2194
                     /locus_tag="PK849_08225"
     CDS             1853..2194
                     /locus_tag="PK849_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66150.1"
                     /translation="MMLARGAIADPFLFPRTLAFLGYEVHNKYRNPSPDFQLSLLLDF
                     GEYVCAGSGPRIAVLMVKRLLSGMFKGLPGIGELRRAAAHILSWKELRGILETCEVYF
                     ERREEHSGCAS"
     gene            2205..3755
                     /gene="lysS"
                     /locus_tag="PK849_08230"
     CDS             2205..3755
                     /gene="lysS"
                     /locus_tag="PK849_08230"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPE66151.1"
                     /translation="MNTVPGGDESGPGDEIVRQRMDKLERLLSEEGYNPYILEKWERK
                     HQLEYVRRNFDHLEADEIDESVSIVTAGRIMTLRKHGKAMFANLADETETMQLYLQFN
                     AMGDEQYTFAKKWVDTGDWVGIVGYPFRTQRGELTIHVRQCVLLCKALRPLPEKWHGL
                     KDTELRYRQRYTDLIANPDVRETFRKRSKIISSIRKTLEDHGTLEVETPILSVLAGGA
                     NARPFKTFHNALGMEMYLRIATELYLKRLVVGMFGRVYEMGKNFRNEGLDTMHNPEFT
                     AMEVYWAYADYEDMMALTEEIIRKAAAVVRGPDSSGPVRYQDVDLDFDKPFKRGSMLD
                     LVREYTGVDFRTIADDDEARRIGKERGVEIKGTESRFTVLNLMFETFVEEKLIQPTFV
                     TGHPTEISPLAKRDPENPDYTRRFELFICGKEVANAFSELNDPIDQRARFEDQLKKKE
                     AGDDEAHDFDEDFINAIETGLPPTGGLGIGVDRLVMFLTDSRSIRDVILFPTMKPLVS
                     KSKDAPEA"
     gene            3775..>4204
                     /locus_tag="PK849_08235"
     CDS             3775..>4204
                     /locus_tag="PK849_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPE66152.1"
                     /translation="MAESTIRRDPEEVRSRVVFLREEILRHEHLYYVLDTPEIPDDQY
                     DGLFAELRRLEEEHPSLQSPDSPTQRVGGKAAEGFAKVRLSVPMLSLDNALDTEELSA
                     FLTRTAPFAGEHGYLCELKIDGLAVSLVYEDGVFARGTTRG"
BASE COUNT          872 a         1162 c         1286 g          884 t
ORIGIN      
        1 ttctctctgg gagtgggttg cagacagcgc ggcatctttc tttccctgga cgctctcatg
       61 gtctccgctg cttgcaacgc ttgggggcgg tctttttctc ttgacagtga caggaggact
      121 ctttcccctg agagggcgga caatagcggg cgccggtatt ctcctgcttg cggctgtgtt
      181 ttttctttaa agggcgcgtt gccgcttgcc tctgttttcc agccttccga gagcgcgcct
      241 cctgagtgtt ccttgaagat atttccggtg gtataatact ctgatgttta aagccttccg
      301 ttcaaggagg aggatatcat gttgcttgtt ctcgacgtgg ggaacaccac gacggtcata
      361 ggaatctatg aaggcgatac tctgaaaaaa cactggcgtc ttatgtccga acgtcatact
      421 tcggatgagt tgggtattta tcttctcaac ctgctttcca tctccggaat ttcgctgaag
      481 gcgctcgaag gcgccatcta ttcgagcgtg gtcccctctc tcgataccgc gctctccgag
      541 gggttggagg agtatcttgg cgtcgttccc gtaaaagtca ccgcaagcct cgaccttggg
      601 atggagatcg cctattctcc gaagcacgag gtgggagccg accggctggt gaattccgtc
      661 gcagggaagg cccgtttcgg cgccccgctc attgtcgtcg atttcggaac ggctatcacg
      721 cttgatattc tcaataaaga aggcgcatat cttggcggca ccatctctcc ggggcttgtt
      781 acagggatgg aagcgctctt cggcaggacg gcgaaactgc cgcaggtctc ccttgaacct
      841 ccgaagagcg tcatcggaaa taacaccata ggctccatac aggccggtat cctgttcggc
      901 aacgccggac tggtcgactc tctcgtacgg aggacatgga gcgaattggg aacgaagacg
      961 cccgtggctg ccaccggcgg acatgcttcg gtcgtcgcgt cgctctcgga gacgatcaca
     1021 catgtcgatc cgtggctgac gcttgagggg ctgcgcctga tctacgaaag gaatcgcgcc
     1081 aggtgaccct tgacgaattg cctgttcctc cgccctctgt cgctgcacat ccggttgaga
     1141 taggggggct tcggcttgcg aacccgctgc ttctcgctcc cttggccgga gtgaccatac
     1201 ccgcccttcg gcttctctac tcaaagctcg gcgccggggc ggtgcatacg gagatgataa
     1261 gctgcgccgg actgctcgta tcggaaagac agtctcggaa gcggaacaac accggagacg
     1321 aggggtatga gatcgtctcc cccggatcga agactgagcg aatgcttgcc gtcctggagg
     1381 gagaatcgcc cgtaatagcc cagctttttg caggagatgc agaaaccatg gccagggggg
     1441 ctgagtgtgc actcggggtt cagagtaaaa tcgtcgctct gggcataaat atggcctgtc
     1501 cgatgcccaa agtactgaag aagggcgccg gttcgaaatt gctggagcgt cccgaggtgg
     1561 ccttcgacat ggtgaggcgg ctggttgccc tcgggcttcc tgtctggccg aagatacgca
     1621 agtgccctcc gacgttgccg ctgacgacgg aggcgttctg cgaagggctt ctggaagcgg
     1681 gcgccgcgtc ggtgaccgtg cacggaagaa cgcccgcgca acgctatacc ggagaagccg
     1741 acgtttcttt gcttccgcgt cttgcaacgc tttttccggg aaaagtctgt gccagcggag
     1801 atgttttctc ccccgaacgg gccgcggaat acctcaacgg aggtgcgccg cagtgatgct
     1861 tgcacgggga gcgattgcgg atccgtttct tttcccgcga acgctggcgt ttctcggcta
     1921 tgaagtgcat aataaataca gaaatccctc cccggatttc caactttccc ttcttctgga
     1981 tttcggcgaa tacgtctgcg ccggatcggg gcccaggatc gccgttctca tggtgaagcg
     2041 gcttctgtcg ggcatgttca aagggcttcc cggaattggc gaactgcggc gcgccgctgc
     2101 gcacatcctc tcctggaagg aactccgggg aatacttgaa acgtgtgagg tttattttga
     2161 aaggagagag gaacatagtg gatgcgcatc atgaacagga agtcctgaac accgttccgg
     2221 gaggagatga gtccggtccg ggcgatgaaa tagtccgcca gaggatggac aagctggagc
     2281 gcttgctctc cgaagagggg tacaacccgt atatcctgga gaagtgggaa cgaaagcacc
     2341 agctggaata cgtccggcgg aacttcgatc acctcgaagc ggatgagatc gacgagtctg
     2401 tttccatcgt cactgcggga agaatcatga cgcttcggaa gcacggaaag gcgatgttcg
     2461 ccaaccttgc cgacgaaacg gagacgatgc aactctacct ccagttcaac gcgatgggag
     2521 atgaacagta cacctttgcg aaaaagtggg tggacaccgg agattgggtg ggcatcgtcg
     2581 ggtatccctt cagaacgcag cgcggggagc tgacgatcca tgtgcggcag tgcgtgctcc
     2641 tctgcaaggc cctccgcccc cttcccgaaa agtggcacgg cctgaaggat acggagctgc
     2701 gctaccgtca gaggtatacg gatcttatcg cgaaccccga tgtgcgcgag acgttccgca
     2761 aacgctccaa gatcatctcc tcgatccgga agacgcttga agaccacggc acgctcgaag
     2821 tggagacgcc gattctctcg gtccttgcgg gaggcgcaaa cgcacgcccg ttcaagacgt
     2881 tccacaacgc acttgggatg gagatgtacc tgcgcatcgc aacggagctc tacctgaagc
     2941 gtcttgtcgt ggggatgttc ggcagagtct acgagatggg gaagaacttc cggaacgagg
     3001 ggctcgacac aatgcacaac cccgagttca ccgcgatgga ggtctactgg gcctatgcgg
     3061 actacgagga tatgatggcg ctcacggagg agatcatcag gaaggccgcg gctgtcgttc
     3121 gcggcccaga ttcctccggt cccgtgcgct accaggatgt ggacctcgat ttcgacaaac
     3181 cgttcaaaag aggctcgatg cttgatctcg tgagggagta caccggagtc gacttccgta
     3241 ccatcgccga cgacgacgag gcccggagga ttgggaaaga gcgcggcgtg gagatcaagg
     3301 gaaccgaaag ccgctttacg gtgctcaacc tgatgttcga gacgttcgtc gaagagaagc
     3361 tcattcagcc gacattcgtg acgggacacc cgacggagat ctcgccgctc gcgaagcgcg
     3421 atccggagaa ccctgactat acccggcgtt tcgagctgtt catctgcggc aaggaagtcg
     3481 ccaatgcatt cagcgagttg aacgatccca tcgaccagag agcgcgcttc gaggaccagc
     3541 tgaagaagaa ggaggccggc gacgacgaag cacacgactt cgacgaggac ttcatcaatg
     3601 cgatcgagac gggacttcca ccgaccgggg gactgggtat cggcgtcgac cgcctggtga
     3661 tgttccttac ggattcgcgc tccattcgcg acgttattct cttcccgacg atgaagccgc
     3721 tcgtctccaa aagcaaggac gcgccggagg cgtagaaaga gaggcgtcgt ccaggtggcc
     3781 gagagcacga tacgccggga tcctgaagaa gtacgttcgc gggttgtttt tttacgagag
     3841 gaaatcctgc ggcatgagca tctgtactat gtactggaca ctcctgagat cccggacgat
     3901 cagtacgacg ggctcttcgc cgaactgcgg cggctggagg aggagcaccc ttctctccag
     3961 tcgccggatt ccccgacgca gcgggttgga ggaaaggcgg ccgaggggtt tgccaaggta
     4021 cggctctccg ttcctatgct cagtctcgat aatgcgctcg atacggagga actctcggcg
     4081 tttctcaccc gtacagcacc cttcgccggt gaacatggct atctttgcga gctgaagatc
     4141 gacggccttg ccgtttctct tgtgtatgag gatggcgtct tcgcgcgggg aacgacacgc
     4201 gggg
//
LOCUS       DAOPKU010000236         4202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23175_length_4202_cov_4.863275, whole genome shotgun sequence.
ACCESSION   DAOPKU010000236 DAOPKU010000000
VERSION     DAOPKU010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23175_length_4202_cov_4.863275"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PK849_08240"
     CDS             <1..247
                     /locus_tag="PK849_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66153.1"
                     /translation="DSVSSWTAVQESYDQRNDDCYYIVTLLKEGAPVRSFMVKVDVGW
                     AGDDWTTPEFARRLEEEIGRAARRGETNTDYPGPLAR"
     gene            575..1612
                     /gene="trpD"
                     /locus_tag="PK849_08245"
     CDS             575..1612
                     /gene="trpD"
                     /locus_tag="PK849_08245"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPE66154.1"
                     /translation="MFAQTLSKVIERKNLESAEMEEAMEALVEGSVPPVQTAAFLTAL
                     RAKGETKEEIGGAVRLLLRKARRVPARRELLMDNCGTGGDCGGTFNISTAAAFVAAGG
                     GIPMAKHGNRAVSSKCGAADVAEALGAEMPDTPERAASCLDAAGLVLLFAPHFHPILR
                     NVAEIRKSLGVRTIFNILGPLLNPAPLTHQVMGVFDPGMAPLVANVLLALGRDRGMVI
                     SGHGGFDELSLSGPNRLVLFSEEGVREAVITPEEAGLARRGDDEFLGGTATENAAIIE
                     DILAGKKGGPRDVVLLNASAVFLVSGMASSWKEGVALASESIDSGRAAEVLEKIRSFS
                     QQREDVIPRSA"
     gene            1628..2386
                     /gene="trpC"
                     /locus_tag="PK849_08250"
     CDS             1628..2386
                     /gene="trpC"
                     /locus_tag="PK849_08250"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPE66155.1"
                     /translation="MILERILHQKMEETNELTAPRRKMSAALKRSGLSVIGEIKRASP
                     SKGIINGGVDPLRQLRLYEQGGADAVSILTDRVFFGGGPEDFSSLRTRTSLPLLRKDF
                     LVSEVQIYESLFLGADALLLIAAALEKERLEALLSLTYSLGMEAIVEVHTEEELLSVL
                     ETDAPILGVNNRNLNDFTVDLRTTARLMDALRSAEGEGSRIVVAESGVRSEEDAGYLA
                     ETGVDAILVGEALMRASNPEETLRSFKRKGRRAA"
     gene            2383..3018
                     /locus_tag="PK849_08255"
     CDS             2383..3018
                     /locus_tag="PK849_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPE66156.1"
                     /translation="MTFVKVCGITRREDIEAAAGLGVDALGFILVPASPRFLPAEEAA
                     ALACTVPEGIRKVAVMVNPSGTEVEAVERTGVFDCIQFHGSETPETLSGCRLETIKAF
                     SVTEEADLENAALFDAASFFLFDSKKRRYDKGVAFDHSMLRGRTFAKPFILAGGLGPD
                     TLRGALREARPFGVDLNSGVEDAPGVKNRVKLAVAVATVRDFDTSERREHR"
     gene            3015..4181
                     /gene="trpB"
                     /locus_tag="PK849_08260"
     CDS             3015..4181
                     /gene="trpB"
                     /locus_tag="PK849_08260"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPE66157.1"
                     /translation="MKTSMYFGIFGGQYVPETLVPALDELETAYLGTLDDASFNGELL
                     ALLHNYSGRPTPLWFAPRITESLGGAKVYLKREDLNHTGAHKINNALGQVLLASKMGK
                     KRIIAETGAGQHGVAVATAAALFGLECVVYMGEEDVHRQAPNVARMNLLGTKVVSVLS
                     GTRTLKDAVNEAIRDWVTNVEDSFYVIGSVMGPHPYPLMVRNFQKVIGEEARAQMLEC
                     EGRLPDQVIACVGGGSNAMGIFHAFVGDRDVELIGVEAAGRGVDTGLHAATISGGKPG
                     VLHGSYSYVLFDEDGQIQPAHSISAGLDYPGVGPEHSHLAATGRATYVSVTDDEAVEA
                     FHRTCRLEGIIPALESSHALAHVYRNAAALPKETIVLVNLSGRGDKDMDTILSL"
BASE COUNT          776 a         1317 c         1404 g          705 t
ORIGIN      
        1 cgattccgtc tcctcctgga ccgccgtaca ggagagctat gaccagcgca acgatgactg
       61 ctactacatc gtcaccctcc tcaaagaggg ggctcccgtc cgcagcttca tggtcaaggt
      121 cgatgtcggc tgggcaggcg acgattggac gacgccggag ttcgcccggc ggcttgagga
      181 agagatcggc cgggcggcgc gccggggcga gacgaacacg gactacccgg gccccctcgc
      241 tcgatgaatg tttccgctta ttgcgtcgtc ttctcaaaag tttcgcttga cagatcttcc
      301 gcaactgtga tatcttctcc gcaatcgaaa tcggggagga cattctcatg attcgtacca
      361 cactcaataa gaattggtgg tggcgggctt gctagcctgc cgccggtgac cataaagctc
      421 gcgatataag aacaacgcgt gttctttaag gccgtggagg caggtccctc aagggatcgt
      481 actctacggc ctttttgtat tcccagcggg ggacagaggc cgaacaggct tctctccccc
      541 gctttctctt tatcagggca aaggaggaaa tagtatgttt gcacagactc tctcgaaggt
      601 gatcgaacgg aaaaatctgg aaagcgcgga gatggaggag gccatggagg cccttgtaga
      661 ggggagcgta cctcccgtgc agacggccgc gtttctgacg gccctccggg cgaaggggga
      721 gacgaaagaa gagatcggcg gcgcggtccg gctgctgctg cggaaggcgc ggagagttcc
      781 ggcgcggcgc gagctgctga tggacaactg cggcaccggc ggcgactgcg gggggacgtt
      841 caacatctcc acggcggcgg ccttcgtggc cgcgggcggc ggcattccga tggcgaagca
      901 cggcaatcgc gcagtctcca gcaagtgcgg cgccgcggat gtcgccgaag cgctgggtgc
      961 ggagatgccc gacacgcccg aacgcgccgc ctcctgcctc gacgctgcgg ggctggtgct
     1021 cctcttcgcg ccgcacttcc acccgatact gcggaacgtg gccgaaatac ggaagagtct
     1081 cggtgtgcgg acgatcttca acatcctcgg tcccctgctg aatcccgcgc cgctgacgca
     1141 tcaggtgatg ggcgtcttcg accccggcat ggccccgttg gttgcgaacg tcctcctcgc
     1201 gctggggcgg gatcgcggca tggtgatctc cggacacggc ggtttcgacg agctctcgct
     1261 cagcggcccg aaccgcctgg ttcttttctc ggaagaggga gtgagggagg ccgtcatcac
     1321 tccggaagaa gcggggctcg cccgcagggg cgacgacgaa tttctcggcg gaacggcgac
     1381 ggagaacgcc gcgatcatcg aggacatcct cgcaggaaag aaggggggcc cccgagacgt
     1441 ggttctcttg aacgcctcgg ccgtcttcct cgtctccggc atggcctcca gctggaagga
     1501 gggggtcgcg ctcgcctcgg agagcatcga ctcaggccgc gccgcggaag tgctggaaaa
     1561 aatccgttcc ttctcgcagc agcgggagga cgttatcccc cgttcggcat gaagggaggc
     1621 cgttgtaatg attcttgaac gcattctgca ccagaagatg gaggagacga acgagctgac
     1681 ggcgccgcgg cggaagatgt cggccgctct caagcgaagc ggactctccg tgatcggcga
     1741 gatcaagcgc gcatccccga gcaaggggat catcaacggc ggcgtcgacc cgctgcgcca
     1801 gcttcgactg tacgagcagg gaggcgcgga cgccgtctcc atcctcaccg accgggtctt
     1861 cttcggcggc ggcccggagg atttcagctc cctgaggacg cggacaagcc ttccgctgct
     1921 tcggaaggac tttctggtga gcgaagtgca gatctacgag agcctcttcc tcggcgcgga
     1981 cgcgctgctg ctcatcgccg cggcgctcga aaaagagcgt cttgaggcgc tgctctccct
     2041 gacatattcg ctggggatgg aggcgatcgt cgaagtgcac accgaggagg agctgttgag
     2101 cgtgctggag acggacgccc cgatccttgg cgtcaacaac cggaatctga acgacttcac
     2161 cgtcgatctg cggaccacgg cgcgcctgat ggacgcgctc cgttccgcgg agggggaggg
     2221 gagccgcatc gtggtggcgg agagcggcgt gcgaagcgaa gaagacgccg gataccttgc
     2281 cgagaccggc gtcgacgcga tcctcgtcgg cgaggccctc atgcgggcat ccaacccgga
     2341 ggagacgctc cgctccttca agagaaaggg gcggcgtgcg gcatgacgtt cgtgaaggtc
     2401 tgcggcataa cgcggcgtga ggatatcgaa gcggctgccg ggctgggcgt cgacgcgctg
     2461 ggcttcatcc tcgtcccggc gagcccgcgc tttctcccgg cggaagaggc cgcggcgctc
     2521 gcatgtactg tcccggaggg catccgcaag gtcgccgtga tggtgaaccc gtccggaacg
     2581 gaggtcgagg ctgtggaaag aacgggcgtg ttcgactgca tccagttcca cggctcggag
     2641 acgccggaga cgctttccgg ctgccgtctt gagaccatca aggcgttctc cgtgacggag
     2701 gaggccgacc tggagaacgc ggcgctcttc gacgccgcgt cgttcttcct cttcgacagc
     2761 aagaagcgcc gctacgacaa gggcgttgcc ttcgaccact cgatgctgcg cggacggacg
     2821 ttcgctaaac cctttattct cgcgggagga ctgggcccgg acaccctgcg gggcgcgctg
     2881 cgggaggccc gccccttcgg ggtcgacctc aacagcgggg tcgaggacgc ccccggcgtc
     2941 aagaacaggg tgaagctcgc cgtcgcagtg gcgacggtcc gtgatttcga cacttccgaa
     3001 aggagagagc accgatgaaa acatccatgt acttcggcat cttcggaggg cagtacgtcc
     3061 ccgaaacgct tgttccggcc ctcgacgaac tggagacggc ctacctcggc acgctggacg
     3121 acgcgtcgtt caacggagaa ctgcttgccc tgctgcacaa ctacagcgga cggccgacgc
     3181 ccctctggtt cgcgccgcgc atcaccgagt cgctcggcgg agccaaggtc tacctgaagc
     3241 gcgaggacct gaaccacacc ggcgcgcaca agatcaacaa cgcgctcggc caggtgctgc
     3301 tcgcctccaa aatggggaag aaacgcatca tcgcggagac cggcgcgggg cagcacggag
     3361 tggcggtggc cactgcggcg gcgctcttcg ggcttgagtg cgtggtgtac atgggggagg
     3421 aggacgtcca ccggcaggcg cccaacgtgg cccggatgaa tctgctgggg acgaaggtcg
     3481 tctccgttct ctccggaacg cgcaccctca aggacgcggt caacgaggcc atccgcgact
     3541 gggtgaccaa cgtcgaggac tccttctacg tcatcggctc ggtgatgggg ccgcacccgt
     3601 accccctgat ggtccgcaac ttccagaagg tcatcggaga agaggcgcgc gcgcagatgc
     3661 tcgaatgcga gggacggctc cccgaccagg tgatcgcctg cgtcggcggc ggcagcaacg
     3721 cgatgggcat cttccacgct ttcgtcggcg accgcgacgt tgaattgatc ggagtcgagg
     3781 cggcgggcag gggagtggac acaggccttc acgccgccac catctcgggc gggaagccgg
     3841 gcgtgctcca cggctcgtac tcgtacgtcc tcttcgacga agacgggcag atacagcctg
     3901 cgcactccat ctccgccggg ctggactacc ccggcgtcgg gccggaacac agccatctcg
     3961 ccgctacggg gcgtgcaacc tacgtctccg tcaccgacga cgaggcagtg gaggccttcc
     4021 accgcacctg ccgcctcgaa gggatcatcc cggcgcttga aagctcgcac gcgctcgcgc
     4081 acgtctaccg gaacgccgcc gcactcccga aggaaacgat cgtgctggtg aacctctccg
     4141 gccgcggcga caaggacatg gacaccattc tgtccctttg aaaggagcta cttcttatga
     4201 ac
//
LOCUS       DAOPKU010000237         4202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23176_length_4202_cov_4.621172, whole genome shotgun sequence.
ACCESSION   DAOPKU010000237 DAOPKU010000000
VERSION     DAOPKU010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23176_length_4202_cov_4.621172"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PK849_08265"
     CDS             complement(<1..1248)
                     /locus_tag="PK849_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66158.1"
                     /translation="MKKVILYISLFCVFGAPFCFANAASAGDAPDAWVKGLWIQANGL
                     PEDASIEDYSDGDDEKPWFVYSWGEGGDGPAVTLAVGRFSASVDAEKKLQNLDKSVLR
                     EFIGSEDFWESSDAKKMTFTKAPEIISASFSYPCQVVRYAESDMGLSHMVLFIETDPF
                     LFMAEVTWETTNRTFKKSDAEKILANLALVEQEGVEAAATDVHAFSLKDERVYKGEEI
                     IEAEVHNVPPEIEGPLRFWSVVGADESSPAAENETGVWFFSEDGAFLTFLPLESAGEC
                     QDIIFSPDGSYFLLISGSGVRADMTYILYELETMEQKIEIPGTRESASWIDVGRFAMT
                     RIDDVRETENGTFSMAALRLSAVMYDTVAEELVVLKEATTMKNYWYGGLSDDGGSFVI
                     QEDSVKSEKDWGDEEKITTREIKI"
     gene            complement(1363..3129)
                     /locus_tag="PK849_08270"
     CDS             complement(1363..3129)
                     /locus_tag="PK849_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66159.1"
                     /translation="MRKRIGTFFVFALAFALFAAPVQAGDGKVKGLWLEIPGLPEASS
                     TTFNIQSDGEGEAYFERDFDGGILKVSIERIYSEDRNGEALGPGDTGKLVVRLQSLRK
                     DIEIDPDDVEITESVEELEEIYSQPVAIALYTTGEDEDMRGNQDIFIFTDEWIFRVHL
                     SLAADHMDDYDADEMKDWFEDMKIFDRDPSRANGSEDESQDRHATRADAGVEGLWLRI
                     PDFPDDAEVLEFTADDDGEVTYIRALDDGALVLGIYRTPACDETTPEKVKESIVESVK
                     DSGGDADDIEFDAGANDFSELLSYPCMTAEYKIGEDEDAKRVAILGVFADDYVFLIQA
                     AGAADTFGEYSDRSEEWFTHMKLVDREEGKGDVFDDETGEDDEKTLDFVAVVYSLPEF
                     RGIAWRIPAPGKYDLGDGFDIPNDSVCSVAVCPGYRVTLYEHSQFGGETAEFEENEGD
                     LEERSRWASSLKVERIEKPDFDLAFEWCMVQAENEGLFEEIDLDTATAEKALEVHAER
                     VERFKKAGEMHWYGTTTDAERVELGGELIKIFSDMGVATDDWEADIFAQAMNNFYDWR
                     KDLSVWDVACMILNVNPETFNQ"
     gene            complement(3365..>4202)
                     /locus_tag="PK849_08275"
     CDS             complement(3365..>4202)
                     /locus_tag="PK849_08275"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPE66160.1"
                     /translation="LFEARSAGQIGNCLCASLVLARIMLLRGDGDSYTSIRRSILKDA
                     QEAQQTSLLRIGDLCQAVTDLALGRLDDLPPWLRDAESIRKVVYTDGQPYAMMLHGMA
                     LLQEERYSELYGIAEPVLEFARRMHYMLPQVYYNIYLAVAKNAEACREEAVGYLEQAL
                     LLALPDEVYLPFAEHGDSLTPLFDSLQSAFPEDRVRAVRSLCARQASGAETVRKTLSG
                     SGSILSARQREVALLLKDGLSVKEISKNLFVTENTVKSITKAVYGKLGVHSRVELTVT
                     SF"
BASE COUNT          819 a         1261 c         1077 g         1045 t
ORIGIN      
        1 tatttttatt tcccgcgttg tgattttttc ctcgtcgccc caatcctttt ccgacttcac
       61 ggagtcttcc tgaatgacaa acgagccgcc gtcgtccgat aatccgccgt accagtagtt
      121 cttcatcgta gtcgcttctt tcagaacgac cagctcctcc gctacggtgt cgtacatcac
      181 cgccgagagg cgaagagcgg ccattgaaaa agtcccgttt tccgtctcgc gaacgtcgtc
      241 gatccgggtc atcgcaaaac ggcccacgtc aatccacgaa gcggattccc tcgtccccgg
      301 gatttcaatt ttttgctcca ttgtttccaa ctcgtacaga atgtacgtca tgtcggcccg
      361 aactccgctt ccgcttatca ggaggaaata actcccgtcc gggctgaaaa tgatgtcttg
      421 gcattcgccg gcgctctcga gcggcaggaa cgtcagaaat gccccgtctt ccgaaaagaa
      481 ccagacgccg gtttcgtttt cagctgcggg gctcgattca tccgccccga cgacgctcca
      541 aaaacgaagg ggtccttcaa tttccggagg tacattgtgt acttccgctt caataatttc
      601 ttcgccctta tacacgcgtt catccttcag tgaaaatgcg tgaacgtcag tcgcagcggc
      661 ttccacccct tcctgctcga cgagagcgag gttcgcgagg attttctccg catcgctttt
      721 tttgaaagtc ctgttcgttg tctcccatgt tacttcagcc ataaaaagaa acgggtcggt
      781 ctctatgaac aggaccatat gggagagtcc catatctgat tccgcgtatc tcacgacctg
      841 gcaggggtag gagaaacttg cgctgattat ttccggggct ttcgtgaatg tcattttttt
      901 cgcgtcggag ctttcccaga aatcctcaga gccgataaat tcgcgaagaa cacttttgtc
      961 gaggttctga agttttttct ccgcgtctac cgaagccgaa aaccgcccca ctgcgagcgt
     1021 tacggcaggg ccgtcgcccc cctcgcccca actgtagacg aaccatggtt tttcatcgtc
     1081 gccgtccgaa tagtcttcga tcgacgcgtc ttcgggcagc ccgttcgctt gtatccatag
     1141 ccctttcacc catgcatccg gtgcatcgcc ggcgctcgcg gcgttcgcga agcaaaaggg
     1201 cgcgccaaaa acacagaaaa gcgaaatgta cagtattacc tttttcattt cactgaaacc
     1261 tccaatctat gtatggtttt gaaccaaatt ccgggggttc gtttgccaaa catgtattca
     1321 gtgcgtatcc tgtttccttt ttttctctcc ggcgctcttt ccctattggt taaaagtttc
     1381 cgggttgacg ttgagaatca tgcacgccac atcccacacg ctcaaatcct ttcgccagtc
     1441 gtagaaattg ttcatcgcct gcgcgaaaat gtccgcttcc cagtcgtccg tggcgactcc
     1501 catatcgctg aaaattttta tcagttctcc gcccagctcg acacgctccg cgtccgtcgt
     1561 cgttccgtac cagtgcatct cacccgcttt tttgaaacgc tccactctct ccgcatggac
     1621 ttcgagggct ttctccgcgg tcgctgtgtc tagatcgatc tcctcgaaga gcccctcgtt
     1681 ttccgcctga accatacacc attcaaaggc aaggtcaaag tccggtttct ctatccgctc
     1741 gactttgagg gacgacgccc agcggctccg ctcttcgagg tctccttcgt tctcctcgaa
     1801 ttccgcggtc tcgccgccga attgagagtg ttcgtagagt gtcaccctgt accccggaca
     1861 tactgctacg gaacacacgc tgtcgttggg aatgtcgaat ccgtcgccca ggtcgtattt
     1921 gcccggcgct gggatgcgcc acgcgatgcc ccgaaactcc ggaagcgaat agacgacggc
     1981 gacgaaatcc agtgtcttct cgtcgtcctc cccggtttcg tcgtcgaaca catcgccttt
     2041 gccttcttcg cggtcgacga gcttcatgtg tgtgaaccac tcttcgctcc tgtcggagta
     2101 ctcgccgaac gtgtccgccg cgcccgcggc ttggatgagg aaaacgtaat cgtcggcgaa
     2161 tacgccgagt atcgcgaccc ttttggcgtc ctcgtcttca ccgattttat attccgccgt
     2221 catacacggg tacgagagca gttcggagaa gtcgttcgcc cccgcgtcga actcgatgtc
     2281 gtcagcgtcg ccgccgctgt ccttcacgct ctccacaatc gattccttta ctttttcggg
     2341 cgttgtttcg tcacaggcgg gcgtcctgta gatgccaagc acgagtgcgc cgtcatcaag
     2401 agcgcgaatg taggtgactt caccgtcgtc gtccgcggta aactctagaa cttccgcgtc
     2461 gtccggaaag tccggaatcc gaagccacag cccttcgaca cccgcgtctg ctctcgtggc
     2521 atgcctgtct tgggattcgt cttcgcttcc gtttgcccgg gatggatctc gatcaaagat
     2581 tttcatgtcc tcgaaccagt ctttcatttc atccgcgtcg tagtcatcca tgtgatcggc
     2641 tgcgagcgag agatgcacgc ggaagatcca ctcgtcggtg aagatgaaaa tgtcctggtt
     2701 ccctctcatg tcctcgtctt cgccggtcgt atacagcgct atcgcgaccg gctgggagta
     2761 aatctcttcg agttcctcga cggactcggt gatttcgacg tcgtcggggt cgatttcgat
     2821 gtccttgcgg aggctttgca gtctgacgac caatttaccg gtatcgccgg gacctaaggc
     2881 ttcgccgttt ctgtcctccg agtaaatcct ttcgatcgat actttcagta tgccgccgtc
     2941 gaaatcccgc tcgaagtacg cctcgccctc gccgtccgac tggatattga atgttgtgga
     3001 acttgcctcc gggagacccg gaatctcgag ccacaacccc ttcaccttgc cgtcgccggc
     3061 ttgtacagga gccgcgaaca gcgcgaacgc cagagcaaag acaaaaaatg taccaatacg
     3121 cttcctcata cttgttcctc cattcctctc gatatgtccg tccttgagcc ggaaggcgtc
     3181 gatattcagt cgacggcgtc ttttcttccg tctctttcgc aaataacggc atcaaggggc
     3241 gacagagcgc ctcttccaag gagacgatac actgctttca aggtttgtgg aatcacccgt
     3301 tcgggtggta ttcccggggg gattccctgg aaggacacgg gagggagcgg ttgaatcgtg
     3361 tttttcaaaa ggaagttact gtcaattcga cgcgggaatg cactcccagc tttccataga
     3421 cggcctttgt tatgctcttc accgtatttt cggttacgaa gaggtttttc gagatctcct
     3481 tgactgaaag gccgtctttc agaagaagcg ctacctcgcg ctgcctggcg ctgagaatgg
     3541 atcccgaccc agagagagtt ttgcggaccg tttccgcgcc cgatgcctga cgtgcgcaca
     3601 gcgaacgcac agcgcgtacc cggtcttcgg gaaaagcgct ttgaagcgaa tcgaacagcg
     3661 gcgtcaacga gtctccgtgt tctgcgaaag gcaggtagac ttcatccggc agcgccagta
     3721 ggagcgcctg ttcaagatac ccgacggcct cttcccggca ggcttcagcg ttcttcgcga
     3781 cggcgagata gatgttgtaa tagacttgcg ggagcatgta gtgcatcctg cgggcgaatt
     3841 ccaggaccgg ttccgcgatg ccgtagagtt ctgaatagcg ctcttcctga agcagcgcca
     3901 ttccgtgaag catcatcgca tacggctgac cgtcggtgta caccactttc cggatgctct
     3961 cggcgtcgcg gagccaggga ggcagatcgt cgaggcgtcc gagcgccagg tcggtaacgg
     4021 cctggcagag gtcgccgatg cggaggagcg atgtctgctg cgcttcctgc gcgtccttca
     4081 gaatgctgcg acgtatggag gtgtacgaat cgccgtcgcc gcggagcagc atgatgcggg
     4141 ccaacaccag cgatgcgcag aggcagttgc cgatctgtcc cgcgctgcgc gcttcgaaaa
     4201 gc
//
LOCUS       DAOPKU010000238         4199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23198_length_4199_cov_5.319015, whole genome shotgun sequence.
ACCESSION   DAOPKU010000238 DAOPKU010000000
VERSION     DAOPKU010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23198_length_4199_cov_5.319015"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PK849_08280"
     CDS             complement(<1..415)
                     /locus_tag="PK849_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPE66161.1"
                     /translation="MKVVFIAEPHSLEIKEMPAPEAGKGEVLLRVRAAGICGSDMHIY
                     HGTNPLAKYPRIIGHEFAGEIAALGEGVEGFSVGDRVTVDPVTSCGECYACSIGRRNV
                     CERLEVFGVHRDGGFAEYVALPASNLHKVPASWSFE"
     gene            complement(444..1916)
                     /locus_tag="PK849_08285"
     CDS             complement(444..1916)
                     /locus_tag="PK849_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPE66162.1"
                     /translation="MFTLLWGGLQTTFSLAIFPFLVLGVTGGIVIGALPGLTATMGVA
                     VLLPLTFGMESTRALVMLSGIYIGAIYGGSISAILLKTPGTPAAAATVLDGYELARKG
                     EAGKALSISAIASFTGGIVSTVMLITISPILARFALRFGAPEYFALAVFGLSIIASIS
                     GKSPVKGLLAGMFGLLISTVGLDPVTSFPRFTFGEINLFNGFAIIPVLIGLFALAEAF
                     VQMEKIFEPAQTVKTDFKRGMVSLKETLSLMPLMIKSALIGTFIGSIPGAGADIAAFV
                     SYNEARRSSKKPEKFGTGCLEGIAAPEAGNNGVTGGAMVPLLTLGVPGDAVTAILLGA
                     LVIQGLQPGPLLFTKNADVIYGLFSSLFVGNFLMLIFGLLGVRLFCRVVELPKRIIIP
                     VIVTLSMVGAYSMNNSIFDVWVCVAFGIIGYIMQKVEMPSSPIILAVILGPMAESNLR
                     RAVLMYEGSYSFLWTRPISVVFLVMAFFSLYSSYKRAHAS"
     gene            complement(1929..2396)
                     /locus_tag="PK849_08290"
     CDS             complement(1929..2396)
                     /locus_tag="PK849_08290"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HPE66163.1"
                     /translation="MKKANYGIVLVFLLLSAFVIYQANNFEQTLIQDDYVGASFFPEL
                     LAWMTAGLALFLGWLNFRGKMDDDGRTLADLFPRQILLAVVGLGLVVGYVMLLEPLGF
                     ILATIALNAALLPLFGVRSSLALALFPVIVSLSVYVVFYKILIVPLPEGLFYF"
     gene            complement(2509..3456)
                     /locus_tag="PK849_08295"
     CDS             complement(2509..3456)
                     /locus_tag="PK849_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HPE66164.1"
                     /translation="MKKLALFATLLLLVFAGTALAADFPAKNIRLLVPFAAGGGTDAV
                     ARALAVAAEKHLGQSVVVMNRTGGSGAVGMTEGATAKADGYTVTMITREIAWLPQMGL
                     AQITPDNFDMIALVNEDPAIMLVQPDSKFTSVKDVIETAKAAPGTVKFGSTAKPNFYL
                     LALELNQNVKFNQIPYNGAAEAIPALLGGHVEFSMVNPGEAISQIMAGQLKPLGVCSD
                     VRMAGLPDVPTMTELGYPIVTGTWRGIAVPKGTPEDVKAILGAALEKAVAEKEFVDFM
                     NGRTLGIRFLNAADFKTFVENDVKALDAVVEEVKKQQQQ"
     gene            complement(3499..>4199)
                     /gene="kduD"
                     /locus_tag="PK849_08300"
     CDS             complement(3499..>4199)
                     /gene="kduD"
                     /locus_tag="PK849_08300"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HPE66165.1"
                     /translation="LGYGIAEGLAEAVANIVGVGPIAMPEIEARVKALGRKFLHVEAD
                     LTSQARIPDIVAETVSAMGGVDILVNNAGIIRRADILEFTEKDWDDVMNVNLKSLFFL
                     SQEAARQMAKQGRGGKIVNIASMLSFQGGIRVPSYTSSKSAVAGLTKLMANEMAQFGV
                     QVNAIAPGYMATDNTEALQKDPVRNKEILARIPNGRWGTPDDLKGAAVFLASAASDYV
                     TGYVVAVDGGWLSR"
BASE COUNT          860 a         1295 c         1287 g          757 t
ORIGIN      
        1 tctcgaaact ccatgaagca ggaactttgt gaaggttcga ggcaggaaga gcaacgtatt
       61 cggcaaaacc gccgtccctg tgcacgccga agacctccag gcgctcgcag acgttgcgcc
      121 gcccgatgga acaggcatag cactctccgc agctcgtcac aggatcgacc gtgaccctgt
      181 cgccgacgga gaatccctca acgccctccc cgagcgccgc gatctccccc gcgaactcgt
      241 gccctatgat ccgggggtac ttcgcaaggg ggttggtccc gtggtagatg tgcatatccg
      301 acccgcagat ccctgcggcg cgcacccgga gaagaacttc ccccttcccg gcctcgggcg
      361 ccggcatttc tttgatctcc aaactgtgcg gctccgcaat gaagactact ttcattctca
      421 ttctctcctt tgtacgaccg aatttacgat gcgtgcgccc gtttgtacga cgagtagagg
      481 gaaaagaacg ccatgacgag gaagacgacc gagatcggcc gcgtccagag gaacgagtag
      541 ctcccctcgt acatcagcac cgctcggcgg aggttgctct ccgccatggg ccccaggata
      601 acggcgagga tgatgggcga cgacggcatt tccacctttt gcattatata ccctatgatt
      661 ccgaaggcga cgcagaccca gacgtcgaag atgctgttgt tcatcgagta cgcccccacc
      721 atcgacaggg tcacgatcac agggatgatg atccgtttcg gcagttcgac cacccggcag
      781 aagagacgca ccccgagcag accgaaaatc agcatcagga agttccccac gaaaagcgag
      841 gaaaaaagac cgtagatcac gtcggcgttc ttcgtgaaga gcagcggccc cggctgcaac
      901 ccctgaatga cgagggcgcc aagcagaatg gcggtcacgg cgtctccagg cacgccgagc
      961 gtgagcagcg gaaccatcgc tccgccggtg acgccgttgt tcccggcctc gggcgcggcg
     1021 atgccctcaa ggcaccctgt cccgaacttc tccggctttt tggaggagcg tcgcgcctcg
     1081 ttgtagctca caaaggcggc aatgtccgcc ccggctccgg gaatggagcc tatgaacgtc
     1141 ccgatgagcg ccgacttgat catcagcggc atcagggaga gggtctcctt cagcgagacc
     1201 attcctctct tgaaatctgt cttcacggtc tgcgcgggtt cgaagatctt ctccatctgg
     1261 acgaaagcct cggccagcgc gaagagaccg atgagcacag ggatgatggc gaagccgttg
     1321 aagaggttga tctcgccgaa ggtgaaacgc gggaagctgg tgaccgggtc gagcccgact
     1381 gtcgaaatca gcaggccgaa cattcccgcc aggagcccct tcaccggact ctttccggag
     1441 atgctcgcga tgatgctcag gccgaagacg gcgagcgcga agtactcggg cgcgccgaag
     1501 cgaagtgcaa agcgggccag tatcggcgag atggtgatca gcatgaccgt gctcacgata
     1561 ccgccggtga acgaagcgat tgcggagatt gagagcgctt tgccggcctc gcccttcctg
     1621 gccagttcgt agccgtcgag aactgtcgcg gctgcggcgg gcgtcccggg agttttcaac
     1681 agaatcgcgg agatggagcc cccgtagatg gcgccgatgt agatgcccga gagcataacc
     1741 agcgcgcggg tggactccat gccgaacgtg agcggaagca gcaccgcgac gcccatggtc
     1801 gcggtgagac cgggaagcgc cccgatgacg atgcccccgg taacgccgag gacgaggaac
     1861 gggaagatgg cgagagagaa ggtggtctga agaccgcccc agagaagagt aaacattcct
     1921 gccgcctcct agaagtagaa gagcccctcc ggaaggggca cgatgaggat tttataaaac
     1981 accacgtaca ccgacaacga gacgatcacg ggaaagagtg caagggcaag agagctgcgc
     2041 acgccgaaga gcggaagcag cgcggcgttc agcgcgatgg ttgccagaat gaaaccgagg
     2101 ggttccagaa gcatgacgta cccgacgacg agccccaatc cgacaacggc aagcagtatc
     2161 tgccgcggga agagatcggc gagcgtccgt ccgtcgtcgt ccattttgcc gcggaagttg
     2221 agccacccca ggaagagagc aagtccggcg gtcatccagg cgagcagttc cggaaaaaag
     2281 gatgcgccga cgtaatcgtc ctggataagg gtctgttcga agttatttgc ctggtagatc
     2341 acgaatgcgc tcagcaaaag aaaaacaagt acgatgccgt agttggcttt tttcacgtga
     2401 cattctcctc cgaaattatc tgtggagcgt tgggaaaggg cgggaggccg ccgtcggaac
     2461 cggcgccccc cgcgtggtta cgtcctgtaa cgaaaacgaa gcgttctctt attgctgctg
     2521 ctgttttttc acttcttcaa cgactgcgtc gagggccttc acgtcgttct ccacgaaggt
     2581 tttgaagtcg gctgcgttca ggaaacggat cccgagcgtg cggccgttca tgaagtcgac
     2641 aaactccttc tcggcgacgg ccttctccaa ggccgcgcca agaattgcct tcacgtcctc
     2701 gggcgttcct ttgggaaccg cgatgcctct ccacgtaccg gtgacgatgg gatatccaag
     2761 ctctgtcatc gtcggcacgt cgggaagccc ggccatgcgc acgtcggagc agacgccaag
     2821 gggcttcagc tgccccgcca tgatctggga gatcgcctcg ccggggttga ccatggagaa
     2881 ctccacatgg ccgcccagaa gcgcgggaat ggcctcggcc gcgccgttgt aggggatctg
     2941 gttgaacttc acgttctggt tcagctcaag ggcgagaagg tagaagttgg gcttcgcggt
     3001 gctgccgaac ttcaccgttc ccggagctgc cttcgccgtc tcgatgacgt ccttgaccga
     3061 ggtgaacttc gaatcgggct ggacgagcat aatcgccgga tcttcgttca ccagcgcgat
     3121 catgtcgaag ttgtccggag tgatctgcgc aaggcccatc tgcggaagcc acgcgatctc
     3181 acgggtgatc atggtgacgg tgtagccgtc ggccttcgcc gtcgcgccct ccgtcatgcc
     3241 gaccgcgccg gaaccgccgg ttctgttcat caccacgacg gactgaccga ggtgtttctc
     3301 ggccgcgacc gcaagcgcgc gcgccacggc gtcggtgccg ccgccggcag cgaagggaac
     3361 gagaagacgg atgttcttcg ccgggaagtc ggccgcaaga gcggttccgg cgaacaccag
     3421 gagcaggagc gttgcgaaaa gtgccagttt tttcattgtg attttcctcc tctatgaatg
     3481 tgaatgtgtg aagccgattt atcgggagag ccagccgccg tccaccgcca caacgtaccc
     3541 cgtcacgtag tcggaggccg ccgaagcgag gaagaccgcc gcgcccttca gatcgtcggg
     3601 agttccccac cgcccgttcg gaatgcgggc gaggatctcc ttgttccgca cggggtcctt
     3661 ctgcagggcc tccgtgttgt ccgtggccat gtagcccgga gcgatggcgt tcacctgcac
     3721 gccgaactgc gccatctcgt tggccatcag cttcgtcagg cccgcgacgg cgctcttgct
     3781 cgacgtgtag gacggcacgc ggatgccgcc ctggaaggag agcatggagg cgatgttcac
     3841 aatcttgccc ccccggccct gcttcgccat ctgacgggcg gcctcctgcg acaggaagaa
     3901 aaggctcttc aggttgacgt tcatcacgtc gtcccagtcc ttctcggtga actccagaat
     3961 gtcggcgcgg cggatgatgc ccgcgttgtt caccagaata tccacgccgc ccatcgcgct
     4021 cacggtctcc gccacgatgt cggggatgcg cgcctgggac gtcagatccg cctccacatg
     4081 gaggaacttc cgcccgaggg ccttcaccct cgcctcgatc tcgggcatcg cgatcggccc
     4141 cacgccgacg atgttcgcca cggcctcggc gagtccctcc gcgatgccgt agcccaacc
//
LOCUS       DAOPKU010000239         4195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23239_length_4195_cov_4.447585, whole genome shotgun sequence.
ACCESSION   DAOPKU010000239 DAOPKU010000000
VERSION     DAOPKU010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23239_length_4195_cov_4.447585"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1135
                     /locus_tag="PK849_08305"
     CDS             167..1135
                     /locus_tag="PK849_08305"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPE66166.1"
                     /translation="MKTTEDHTPPQTAGDAKGLVPVNTDFIKGVIPPIVTIVDENEKI
                     DEKRMRRQVDFVIEGGVHGILAFGSNGEFYMLDEDEMERGLGIILDQTKKRVPVYFGM
                     GAVKTRKCVQLARMAADMGADGVSVLQPMFLKPTDDELYLHFKTIAGAVPGTPVLLYN
                     NPGRTGYSLSAPLVGRLANDVENIVGIKDSSGDMTLTSEFLRLTKTKEKDFKVFGGKD
                     TLIYGALAHGADGAVATMANFCPELVVSIYEKYVAGDLKGSLEAQFRLNPVRLAMDRA
                     SFPVATKDMAKLLGLDVGEPFRPNLSSAGDVLEFMKKRMQQGGLLP"
     gene            1319..1795
                     /gene="fliS"
                     /locus_tag="PK849_08310"
     CDS             1319..1795
                     /gene="fliS"
                     /locus_tag="PK849_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPE66167.1"
                     /translation="MFWPKNRSRAQETYTENAIRTASREQLLLITYDIGIQSCLAAES
                     ALSNHLYDETNEHLKRAQNVVRELMITLRVNKDNPVTTNLMRLYDFMYTTLIKANTAK
                     DPEKIAEVRAMLEDLKATWEESIVKIAEEYDESKKSEYLNNLPSVQRNGDRFSARG"
     gene            1966..3252
                     /locus_tag="PK849_08315"
     CDS             1966..3252
                     /locus_tag="PK849_08315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE66168.1"
                     /translation="MSTSRSTYRSLFADPSFPLFIILTSSFFLTVFFRISASVILPLE
                     GERLGMSASLVGFISSVHFYAYALMQPVSGILHDRYGPVRVVTCGLLLTAASCLLLTI
                     VRTPFTLGVWRLVSGFGVSPMYSATLVFLAFAFPAERYCFYAGINFAVSSLGAIVSVA
                     PLGFALDTFGIPSTFAILAGTSFLMAVFLARRAKSDPMRKAAGDEKKTLSSILPGIGR
                     AVLFIFRNRRVRSLMIVWAVSSASLLSFQGLWGVSWFAAAFNASPASARFWSSLISAG
                     MMVGPLMIGGVVLSPEKLPLLVRRVSSVNALVWFLLLGTTVSGLPIWSGGAVALLLGA
                     ITGLRGVFALAGVTALAPVGEKGVVFGAMNMIAVLSAVVFQWGTGIVINRFPAGQPGV
                     YTAEGYFAAFLLVSCAMAASLLALRSLGKEPLRQEE"
     gene            complement(3346..3459)
                     /locus_tag="PK849_08320"
                     /pseudo
     CDS             complement(3346..3459)
                     /locus_tag="PK849_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
     gene            complement(3445..3903)
                     /locus_tag="PK849_08325"
                     /pseudo
     CDS             complement(3445..3903)
                     /locus_tag="PK849_08325"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(3982..4149)
                     /locus_tag="PK849_08330"
     CDS             complement(3982..4149)
                     /locus_tag="PK849_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66169.1"
                     /translation="MHGTKKETICYCFNYTRSDIEADLLINGRSTILERILAAKKTSG
                     CRCAELNPSGG"
BASE COUNT          780 a         1265 c         1283 g          867 t
ORIGIN      
        1 cggggcggat atccggaccg tgggcgaacg gctcttccga gagatcgtgg aggtcgcaag
       61 cgggcgaacg cacccggcgg ccgaacgcta ccggctgttc aacgacatct gccttttcaa
      121 cccggccccg atcacgtagc actccccgcg ggagcttccc cgaggggtga agacgactga
      181 agatcatacc ccgccgcaaa cggcgggcga cgcgaaagga ctggtacctg tgaacacgga
      241 cttcatcaag ggggtcatcc cccccatagt gaccatagtg gatgaaaacg aaaaaatcga
      301 cgaaaagagg atgcgccggc aggtggactt cgtcattgag ggcggcgtcc acggtatcct
      361 cgccttcggg agcaacggcg agttctacat gctggacgag gacgagatgg agcgggggct
      421 cgggattatt ctggaccaga cgaagaagcg cgtccccgtc tacttcggca tgggcgccgt
      481 caagacgagg aagtgcgtcc agctcgcccg catggccgcg gacatgggag cggacggcgt
      541 ttccgtgctg cagccgatgt tcctgaagcc gaccgacgac gagctgtacc tccatttcaa
      601 aaccatcgcc ggggccgtcc cgggtacgcc ggtgctgctg tacaacaacc cggggaggac
      661 agggtactcg ctctccgcgc ctcttgtggg gcggctcgcg aacgacgtgg agaacatcgt
      721 gggcatcaag gactccagcg gcgacatgac gctgacgagc gagttcctcc ggctgacgaa
      781 gacgaaagag aaggacttca aggtcttcgg gggaaaggac acgctgatct acggcgcgct
      841 cgcccacggc gcggacggcg cggtcgccac catggcgaac ttctgtcccg agcttgtggt
      901 ctcaatctac gagaagtacg tcgcgggcga cctgaagggc tctcttgagg cgcagttccg
      961 gctgaatccg gtgcgcctcg ccatggaccg ggcgagcttc cccgtcgcca cgaaagacat
     1021 ggcgaagctg ctggggctgg atgtcggcga gcctttccgc ccgaacctga gcagcgcggg
     1081 cgacgtgctg gagtttatga aaaagagaat gcagcagggc ggactgctgc cgtagcgcct
     1141 ccccgaaaaa atgcagaggg cgtatccgcg gaaaattgcg ggcacgccct ctgtgaactc
     1201 tcttcctgtt gctgtctgtt cagacttaac ctgcccctcc gctctaccga aaataaaaga
     1261 catcgaggaa actctttgaa cgttttgcga aaatctccgc gggagggggc gagttgtcat
     1321 gttctggcca aaaaacagga gccgggcgca ggaaacctat actgaaaatg ccattcgcac
     1381 cgcgtcgcgc gagcagctcc tgctgattac ctacgacata ggcattcaga gctgcctggc
     1441 cgcggagtcc gcgctgtcga accatctcta cgacgagacg aacgaacacc tcaagcgggc
     1501 gcagaacgtc gtgagggagc tgatgatcac gctgcgcgtg aacaaggata atccggtcac
     1561 gacgaacctc atgcgcctct acgacttcat gtacacaacg ctcatcaagg cgaacaccgc
     1621 gaaggatccg gagaagatcg cggaggtgcg cgcaatgctc gaagacctga aggcgacctg
     1681 ggaagagagc atcgtcaaga tagcggagga gtacgacgaa agcaagaaga gcgagtatct
     1741 gaacaacctg ccctcggtgc aacgaaacgg cgacaggttt tcggcccggg ggtagagggg
     1801 ggcgaagttc cagcgaggcg gacgctccgg aaaagaatag cagcaacacg gaagaagctg
     1861 gttatttctc ttcattataa gtatgtttcc cgacttcccg cagttcattt tctatggtat
     1921 gctgtacccc gttcaaaaca ttgactgaga caaaggagct ttgctgtgag cacttctcgt
     1981 tcgacttaca ggtcgctttt tgccgacccg tcctttcccc tgttcatcat actgacctca
     2041 tcgtttttcc tgacggtctt cttccggatt tcggcctccg ttattctgcc gctggaagga
     2101 gagcggctcg gcatgagcgc gtcgctggtg gggttcatct ccagcgtcca tttttacgcg
     2161 tacgcgctga tgcagcctgt ctccggcatt ctgcacgaca ggtacggccc ggtgcgggtg
     2221 gtgacctgcg gtctgctgct caccgcggct tcgtgcctgc tgctgacgat tgtccggacg
     2281 cccttcacgc tcggcgtctg gcggctggtc tccgggttcg gcgtctcccc catgtactcc
     2341 gcgacgcttg tctttttggc cttcgccttc cctgcggagc ggtactgctt ctacgcgggg
     2401 atcaacttcg ccgtctctag cctgggggcg attgtctcgg tcgcgcccct cggattcgcg
     2461 cttgatacgt tcggcatccc ctcgaccttc gccattctcg cgggaacgag ctttctcatg
     2521 gcagtctttc tggcgcgcag ggcgaagtcg gaccccatgc gcaaggcggc gggagacgag
     2581 aagaagacgc tctcctccat tcttccgggg atcgggaggg cggtgctctt catcttccgg
     2641 aaccggcgcg tgcggtcgct gatgattgtc tgggccgttt cctcggcgtc gttgctgagc
     2701 ttccaggggc tctggggcgt ctcctggttt gcggcggcct tcaatgcctc gccggcgtcg
     2761 gcccgcttct ggagttcgct gatcagcgcg gggatgatgg tcggtcctct tatgatcgga
     2821 ggagttgtcc tctccccgga aaagctgcct ctgcttgtcc gccgtgtaag ctcggtgaac
     2881 gcgctggtct ggtttcttct gctcggaacg accgtctccg gtcttcccat atggtcggga
     2941 ggagccgtgg cgctgctcct cggcgccatc acgggtttgc ggggagtctt cgctcttgcg
     3001 ggggtgaccg cccttgcccc ggttggggag aaaggggtcg ttttcggcgc gatgaacatg
     3061 atcgccgttc tctcggcggt tgtcttccag tgggggacgg ggatcgtgat caatcgcttc
     3121 ccggcgggac agcctggcgt ctatacggcg gagggctatt ttgctgcgtt cctgctcgtt
     3181 tcgtgcgcga tggcggccag cctgctcgca ctgcgttcgc tcgggaaaga accgctgaga
     3241 caggaagaat aagccgaaag atccgccgca acgcacacaa gtaaagacgc aacaacacac
     3301 atcgcgatcg ctctcttcat tgcgcggaaa gtatttccac gcatattaaa gcgattctct
     3361 gtcctcgtgt tccccgatcc tccgcaccac cctgcacggg ttgcccgcag cgaagacgcc
     3421 ttcggggatg tccttcgtca cgacgcttcc ggcgccgatg acggagcggg agccgatcgt
     3481 cgcaccgggg cagatgatcg cgcccccgcc gacccagacg tccgatccga taaagacggg
     3541 ttttccgaac tccttccccc gccgcgcctg cgcgtccatc ggatgggtcg ccgtgtagat
     3601 ctgcactgcc gggccgaaca tcgtgaagtc gccgatgcgc acttcgcaga cgtcgaggac
     3661 gatgcagctg aagttgaagt acactttgtc ccccaggtga atgtgcacgc cgtagtcgca
     3721 gtggaagggc ggctgcacag tcgcgtgctc tccgccgctc ccgaggagct cctgcaggat
     3781 aagttgccga agcttctgtt ccgtcgcgcg cgatgcgttc agatcgaggc acagatcacg
     3841 cgcccgagct ctcaagcgca caagctcgcg gtcggatggg tcgtacagtt cgcctgcgag
     3901 catcttttcc cgttcggtca ttcgtgttca cactcctcgt tttcggatct cgtccagcac
     3961 acggccgacg tcgcctacgc atcagcctcc cgaagggttc agctccgcgc aacggcatcc
     4021 cgatgtcttc ttcgcggcca ggatgcgttc caggatcgtc gagcgtccgt taatcagaag
     4081 gtcggcttcg atatcgcttc gtgtatagtt gaagcagtag cagattgttt cctttttcgt
     4141 accgtgcatc gctgaccctc cttctcttca attccggaaa gattcccctg cccgt
//
LOCUS       DAOPKU010000240         4193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23257_length_4193_cov_4.732238, whole genome shotgun sequence.
ACCESSION   DAOPKU010000240 DAOPKU010000000
VERSION     DAOPKU010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23257_length_4193_cov_4.732238"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /gene="pgm"
                     /locus_tag="PK849_08335"
     CDS             <1..1465
                     /gene="pgm"
                     /locus_tag="PK849_08335"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HPE66170.1"
                     /translation="VSAAIVEYRKSAGITGPLFVGKDTHALSEPAFRTAVEVFAAAGV
                     RTVLQKGHGYTPTPVISRAILQWNKGRSEGLADGVVITPSHNPPEDGGFKYNPPSGGP
                     ADPEMTDAIQKRANELLRSGTDAVRRIPFEKALGAPCVLEEDYVLPYVDDLASVLDME
                     AIARSGIRIGADPMGGSGVAYWSVIAERYGLDLEVVNPSVDPTFSFMSVDWDGKIRMD
                     CSSPYAMAGLVELKDRYDIAWGNDSDFDRHGIVVPDRGLLPPNAYLAVAVEYLFTHRP
                     GWGSDAAVGKTLVSSSLIDRVAHSVGRKCVEVPVGFKWFVEGLLNGSMGFGGEESAGA
                     SFLRRDGTVWSTDKDGIILNLLAAEMLAVTGEHPGKRYDAITARLGEPFYARIDAPAS
                     TERKKLLKTLSPEAVKASTLAGEPIVARLTAAPGNGAPIGGLKVTSENGWFAARPSGT
                     EDVYKIYAESFLGAEHLTRIQSEAREIVDGVFAAAGV"
     gene            complement(1581..1793)
                     /locus_tag="PK849_08340"
     CDS             complement(1581..1793)
                     /locus_tag="PK849_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66171.1"
                     /translation="MQNTRLNEASPVLSVLAVQGGRVTGKTAKERPKRDGRKRGFDDY
                     LRKAGYESRFDQELEDRLTESIDTLA"
     gene            complement(2333..3580)
                     /locus_tag="PK849_08345"
     CDS             complement(2333..3580)
                     /locus_tag="PK849_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HPE66172.1"
                     /translation="MNAALLKKWFQTLAPIGALSEGGVTRPAYSAEEDLMFRATADIA
                     RRIGLSVSEDCAGNMFISFPGREKEPCHLIGSHLDSVPGGGRFDGVAGVLAALLVMEK
                     IRRNGEDLPVRAVAFRAEESSLYGLATAGSGVITGSVNIAHLKNARSLSGESLYDAMI
                     RKGYTPGACRLEHALDFMELHIEQGRVLFEAKEPLGVVTAIAAPTRLKVTVLGRQDHS
                     GATPMDLRKDALCAAADIILATEAAGRDEMRFATVATVGVANVVPNALNVVPGSVELR
                     IDIRGIQRESIRRTVGKVQRAIEEIAASRGLSCATETISSMDPVTLAPSMIDSLAEAA
                     SGTGVPFRKMASGAGHDAMKVAPVVPTGMLFIPCDEGISHNSAENASLNDIALGAEVM
                     LRSISLRSKHLSTADRSQPFSKL"
     gene            3703..4056
                     /locus_tag="PK849_08350"
     CDS             3703..4056
                     /locus_tag="PK849_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66173.1"
                     /translation="MKKVMLCCLLAVFAVLAVSGSGMAHPPSDLVLSYNRADGMLAVK
                     APHSVPDGKRHYIEEFKVSVDGQKYYELDVEFQIDNKTSLAVFHVGDLKAGAVVEVYA
                     KCNRSGDMTKSLTVE"
BASE COUNT          844 a         1187 c         1268 g          894 t
ORIGIN      
        1 ggttagcgcg gcaatcgtcg agtaccggaa gagcgcgggg attacagggc cgctcttcgt
       61 tggcaaggat acgcacgccc tctccgagcc ggcgttcagg acggctgtgg aagtttttgc
      121 cgccgccggc gtccggacgg tactacagaa aggacacgga tacacgccga cgccggtaat
      181 ttcgcgcgcc attctccaat ggaacaaggg gagaagcgag ggactcgccg acggcgtcgt
      241 catcactccg tcgcacaacc cgccggagga cgggggcttc aagtacaacc ccccgtcggg
      301 cggaccggcg gaccccgaga tgaccgacgc aatccagaag cgagccaacg agctgcttcg
      361 ctccggcacg gacgcggtcc ggcgcatccc gttcgagaag gcgctcggcg ctccgtgcgt
      421 ccttgaagag gactatgttc tgccgtacgt ggacgacctc gcctctgtcc tcgacatgga
      481 ggccatcgcc cggtccggga ttcgcatcgg cgccgacccg atggggggct ccggcgtggc
      541 atactggagc gtgatcgccg aacggtacgg gctcgacctc gaagtggtga acccctcggt
      601 cgacccgaca ttttccttca tgtccgtgga ctgggacggc aagatccgta tggactgctc
      661 ctctccctac gcgatggccg gactggtgga gctgaaggac aggtacgaca tcgcctgggg
      721 aaacgactcg gatttcgaca ggcacggcat cgttgttcct gacagggggc tgcttccgcc
      781 gaacgcgtac cttgccgtcg ccgtggagta tctcttcacg caccgtccgg ggtggggttc
      841 ggacgccgcc gtcgggaaga cgctcgtctc cagttcgctg atcgacaggg ttgcgcatag
      901 cgtcggaagg aagtgcgtcg aggttccagt cgggttcaag tggttcgtcg aagggctgct
      961 gaacggatcg atgggattcg gcggcgagga gagcgcgggg gcgtccttcc ttcgcaggga
     1021 cgggacggtc tggagtaccg acaaggacgg tattatcctc aatctgcttg cggcggagat
     1081 gctcgcagtc accggagagc atcccgggaa acgttacgac gccatcacgg cccgccttgg
     1141 agagcctttt tacgcgcgca tcgacgcgcc tgcgtcgacg gagcggaaga agctgctgaa
     1201 gacgctctct cccgaggcgg tgaaagcgtc gacgctcgcc ggagagccga tcgttgcccg
     1261 gttgacagcg gcccccggaa acggggcgcc catcggcggc ctgaaagtga cgtccgaaaa
     1321 cgggtggttc gcggctcggc cgtcggggac cgaggacgtg tacaaaatct atgcggagag
     1381 cttcctgggg gcggagcatc ttacccgtat ccagagcgaa gcgcgcgaga tcgtggacgg
     1441 agtgttcgcg gcggccggag tctgatactc tgatttttca gcaagcggcc tccgcgcacg
     1501 atcggacgcg gaggccgctg ttctttttct atgttccgaa ctcttccgga gacgtcttcc
     1561 tctgttcgct gttccgttgt ttatgcaaga gtgtcgatgc tttcggtgag acgatcttcc
     1621 agctcctggt caaaacggga ctcatacccg gctttgcgga gatagtcgtc aaaaccgcgt
     1681 tttcttccgt ctcttttcgg gcgctccttc gccgtctttc cagtgacccg ccctccttgg
     1741 accgcgagaa ctgaaaggac tggagacgcc tcgttcaacc gcgtattctg catggcgctc
     1801 cccccttata acaatgtatc ggtagtgcgc gaagagtttg ttaattccgg atcaggaggg
     1861 ggataacacg gaaggcggct ctcatgagcc gccttcctac gcgtgcagaa aaaaacgatg
     1921 tggaaaaaat ctgtagagta gagaggaaga atgactacgc ttcctcgttg tttctgcttg
     1981 cagagcgttt tttcactaca agcatgacga cgccgataac agccatgatc ccaagccaga
     2041 tgacagtggc ggtgttcata gataacacct cctttttcaa aactttggaa gagctgtggc
     2101 tcaaatacac ctcagaaaac ccgcgcggaa aaacgaggtt gaaaaaattg aaagagagtc
     2161 ggagaactgc atcgatgttt cgttcttaaa ctaccataat aggcagttta attcaagtca
     2221 aaatgataaa tttcatcaat ttactttttt tgctcttcag atagtaatct ttatttatgt
     2281 acttgtagtg tattgggaaa aattgcccgc gacgctcggt tttcgatatc ctctacagct
     2341 tcgaaaacgg ttgagagcga tcggcagtag aaagatgttt gctcctgagg gagatgcttc
     2401 ggagcataac ctctgcgccg agcgcaatat cgttgagcga ggcgttttct gcggagttgt
     2461 ggctgatccc ctcgtcgcag gggataaaga gcatgcccgt cggtacgacg ggggccacct
     2521 tcattgcgtc atgccccgcg ccgctcgcca tcttccggaa gggaacgcct gtcccggaag
     2581 ctgcctcggc gagcgaatcg atcattgagg gagcgagcgt caccggatcc atggaggaga
     2641 tcgtctccgt cgcacacgaa agacctcgcg aagctgcaat ctcctcgatg gcgcgctgga
     2701 ctttcccgac cgttctcctg atgctctcgc gctgtattcc cctgatgtcg atccggagtt
     2761 ccacgctccc ggggacgacg ttgagcgcgt tcgggacgac gttcgcaacg cccacggttg
     2821 cgacggtagc gaagcgcatc tcgtcacgcc ctgccgcttc ggtcgcgaga ataatatcgg
     2881 cagccgcgca gagcgcgtcc ttacgcaggt ccatcggggt tgctccggag tgatcctggc
     2941 gtccgagaac cgttaccttg aggcgcgtgg gtgcggcgat cgccgtgacg acgcccagcg
     3001 gttcttttgc ctcgaagagc acccggccct gctcgatatg cagctccatg aagtcgagcg
     3061 catgctccag tctgcacgct cccggcgtgt accccttccg tatcattgcg tcgtacagac
     3121 tctctccgga aagcgaccgg gcattcttca agtgcgcgat attcacgctt ccagtgatga
     3181 cgccgcttcc cgccgtggca agtccgtaga ggctcgactc ctcggcccgg aaggcgacgg
     3241 cgcgcacagg tagatcttcg ccgtttctcc ggatcttttc catcacgagg agcgcggcga
     3301 gtacgcccgc cacgccgtcg aaacgcccgc cgccgggaac cgagtccagg tgcgagccga
     3361 tcaggtggca cggttctttt tcgcgtcccg gaaatgagat gaacatattt cccgcgcagt
     3421 cctcgctgac ggagagtcct atccggcggg cgatgtctgc tgtagcgcgg aacatgagat
     3481 cctcttcggc cgagtaggcc ggccgggtga cgcctccttc ggagagcgcg ccgatgggtg
     3541 cgagcgtctg aaaccatttt ttcaggagtg ccgcgttcat tcgatcacct cgttgtattg
     3601 cagccgaatc ttctcaatac atttgcttcg tgcgctgcgt gtgtgataaa attaccatgt
     3661 tgtagcagtt caaatcgtac agaaaaggga gagtgaggta caatgaaaaa ggtcatgctc
     3721 tgctgtcttc tcgcagtatt tgcggtgctc gccgtctccg gctcaggaat ggcgcatccg
     3781 ccttccgacc tggtgttgtc ctacaatcgc gccgacggaa tgcttgccgt gaaggcgccg
     3841 cattccgtac ccgacggaaa aagacattat atcgaagaat tcaaggtctc ggtagacggg
     3901 cagaaatatt acgagctcga cgtcgagttt cagatcgaca acaagacctc tcttgccgta
     3961 tttcacgtcg gcgatttgaa ggccggggcg gtcgtcgagg tgtatgcgaa gtgcaaccga
     4021 tcaggggata tgacgaaatc cctgacggtg gagtaggcgc ggagaggttc ctctttgaaa
     4081 gaaaaacagg ttgcaaaaga cgccctttga aggggcgtct ttttcttgac attgaaatgg
     4141 ggaaaaaata gtatatccgg ggcggtttgt gtactgcacc cgcctgcgcc ccc
//
LOCUS       DAOPKU010000241         4174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23420_length_4174_cov_4.373877, whole genome shotgun sequence.
ACCESSION   DAOPKU010000241 DAOPKU010000000
VERSION     DAOPKU010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23420_length_4174_cov_4.373877"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PK849_08355"
     CDS             complement(<1..879)
                     /locus_tag="PK849_08355"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HPE66174.1"
                     /translation="MYFIEGQSHDPAYNLALEEHLYRSVNEGHPGYFLLWQNAPSIIV
                     GRFQNTAQEVNRPFVEERGIHVVRRISGGGAVYHDLGNVNYSFIVPNDEGTPFDFARH
                     ARPVIDALAKLGVEAESGGRNDLLIDGRKFSGSAQHMDRRRLLHHGTLLFDSDLSILE
                     QALSVDEEKFTSKGFKSVRSRVTNILPMLTEPISVEEFIDALRGALSGTETYSLDETQ
                     RTEIRRLRDEKYATWEWNWGASPEFTERKVRRFPWGKVEALLNVKEGVIRQARFYGDY
                     FGTENREEIERLLEGRV"
     gene            972..>1524
                     /locus_tag="PK849_08360"
     CDS             972..>1524
                     /locus_tag="PK849_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE66175.1"
                     /translation="MLDSLEIITLMEDSVPMNGHLVAEHGLSFFIKGTKDGKEVRGLL
                     DVGQTAEFLAHNMKELGVCPQSVDFVVLSHCHYDHTGGIAKFVESTGKHDFPVIANPA
                     LFRPHFVMEPVVAAKGVRTGDEREAIERADGMLLLSGAPFPLADGLSTTGEIPRETDF
                     EGPGKRFFTVRDGEVVQDSLPDDM"
     assembly_gap    1525..1624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1625..1996
                     /locus_tag="PK849_08365"
     CDS             <1625..1996
                     /locus_tag="PK849_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE66176.1"
                     /translation="FFTVRDGEVVQDSLPDDMGIVARVKGKGLVVLAGCSHSGIVNIL
                     KRVRALYPGEPVEGVVGGLHLVNGTEEKMEKTLAGLKEFDPAWIACGHCTGFPMQAKL
                     FQAFGKRFIPLTVGKRFIVGA"
     gene            complement(2092..2868)
                     /locus_tag="PK849_08370"
     CDS             complement(2092..2868)
                     /locus_tag="PK849_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPE66177.1"
                     /translation="MGNNSVFAVFDLETNGFKGASTVSASSIVFTAEGRIVDFFNRFY
                     HSEERPDHRTERVHGLTPERIAHFREEERYPSYFLEDWPSLAAFWERWNPEGIVVHNL
                     SFDLSFLPREAVRGRKWWCSMRGLTDFCKIPKNTDREGEWKWPRLSEAVRIARERLLE
                     PLPLAEAEEFVGPALGHYGLSDCFELYGLFVRVLNQAPERVRFRAASALQLSPRRELY
                     PLPSAVRDSFVRERLDYSARIAEAAGDAERSRKLRSLGTD"
     gene            2990..3586
                     /locus_tag="PK849_08375"
     CDS             2990..3586
                     /locus_tag="PK849_08375"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPE66178.1"
                     /translation="MNDAPFNALRNEVVACERCPLAETRLHAVFGEGPERTPVVLVGE
                     APGAKEDESGRPFVGLSGRLLSELLEEAGLPREKLFITNTVKCRPPENRLPSKAETAA
                     CRPFLAAQLRHIRPRLVITAGNAPTRALLGTKEGITTLRGRLHTCVWEGMELSVLPLF
                     HPSYLLRNRGRAEGSPIMLTLADIREALRLARQFTPGF"
     gene            complement(3570..>4174)
                     /locus_tag="PK849_08380"
     CDS             complement(3570..>4174)
                     /locus_tag="PK849_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPE66179.1"
                     /translation="CHNPELVLPGAANERIAEEHILADLKKRAPFLDGVCVTGGEPAL
                     QPGLLPFLREIRSLGLKVKLDTNGARPDVLERILAEKLADYAAMDLKAPEETYSLACG
                     RDGDATLLPLVRKSIALLLRGAVPFEFRTTLVPGLHRIEDAPLLGRMAEGAPLFVLQN
                     FRPGNTLDPGFSSLSSFPMEFLEEFRTALLPFVGEVKIRA"
BASE COUNT          756 a         1242 c         1299 g          777 t
ORIGIN      
        1 gactcgtcct tcgagcagcc tttcgatctc ttccctgttc tccgtgccga agtagtcgcc
       61 gtagaatcgc gcttgccgta tgacgccctc cttcacgttc agcagcgcct caaccttccc
      121 ccacgggaag cggcggacct tgcgctcggt gaactcgggg gacgcccccc agttccactc
      181 ccacgtcgcg tacttctcgt cccgaagacg gcggatctcc gtacgctgtg tctcgtcgag
      241 cgaatatgtt tcggtcccgg agagagcgcc ccgaagcgcg tcgatgaact cctccacgga
      301 gatgggctcc gtcagcatgg ggaggatgtt ggtcactcgg ctccggaccg acttgaaccc
      361 cttggaggtg aacttttcct cgtccacgct gagggcctgt tcaaggatcg agaggtccga
      421 atcgaagagc agcgtcccgt gatggagcag tctgcgccgg tccatgtgct gggcgcttcc
      481 ggagaacttt cgcccgtcga ttaaaagatc attcctgccg ccgctctcag cttcgacgcc
      541 gagttttgcg agcgcgtcga ttactggccg ggcatggcgc gcaaagtcga agggggttcc
      601 ctcgtcgttg gggacgatga acgagtagtt cacgttcccg aggtcgtgat agaccgcgcc
      661 tcctccggat atccgtctga cgacgtgaat gccgcgctcc tcgacgaagg ggcggttcac
      721 ctcctgagcc gtgttctgga agcgccccac gatgatcgag ggagcgtttt gccacagcag
      781 gaagtagccg gggtgtcctt cgttgacgct cctgtagaga tgctcctcga gcgcgaggtt
      841 gtacgcgggg tcgtgcgatt gtccttcgat aaagtacatt tcgatttctc tcctctcgtt
      901 ccggcgcctc ttgcaaagtc ggcggtttca gttcattata caaaatatcc tgtatccgga
      961 ggtggagcga tatgctcgat tcacttgaga ttatcacatt gatggaagac tcagtgccta
     1021 tgaacggtca ccttgtcgcc gagcacggcc tttcgttctt tatcaagggg acgaaagacg
     1081 gaaaggaggt ccgaggtctc ctcgacgtgg ggcagactgc ggagttcctg gcgcacaaca
     1141 tgaaagagct tggggtttgc ccgcagtcgg tcgactttgt tgttctctca cactgtcact
     1201 acgaccacac cggaggtatc gcgaagttcg ttgagtccac agggaagcat gacttcccgg
     1261 tgatcgcgaa tcctgcgctc tttcgtccgc actttgtaat ggaaccggtt gtggcggcga
     1321 agggcgtccg taccggcgac gagcgcgaag ctatcgagag ggccgacgga atgctgttgc
     1381 tctcaggcgc gccgtttcct cttgccgacg gactgtcgac cactggagag attccgcgcg
     1441 agaccgattt cgaggggccg gggaagcgtt tcttcacggt acgcgacggc gaggtggtcc
     1501 aggactccct ccccgacgac atggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnttcttc acggtacgcg acggcgaggt ggtccaggac tccctccccg acgacatggg
     1681 gattgtcgcc cgcgtcaaag ggaaggggct cgtcgtcctc gccgggtgca gccattccgg
     1741 gattgtgaac atcctgaagc gggtccgcgc gctctacccc ggcgagccgg tcgaaggcgt
     1801 cgtcggcgga ctgcacctcg tgaacggaac cgaagagaag atggagaaga cgcttgccgg
     1861 gctgaaagag ttcgacccgg catggatcgc ctgcgggcac tgcaccggtt ttcccatgca
     1921 ggcgaagctc ttccaggcgt tcgggaaacg ctttattccg ctcacggtgg ggaagcgctt
     1981 catcgtcggc gcgtaagcgg cgagcagctc ccggacgaca gaggggacgg ggcgccagct
     2041 tcccttcatc cgtagagaat acgacgccct gtcctcttat ggagcggtct ctcagtcggt
     2101 tccaagcgag cggagttttc ggctccgttc ggcatctccc gccgcttcgg cgatgcgcgc
     2161 cgaatagtcg agacgctctc tgacgaacga gtctcgaacg gccgaaggca gagggtagag
     2221 ctcacgccgg ggggagagct gcaacgcgga ggcagcgcga aagcgcaccc gttccggcgc
     2281 ttggttcagg actctgacga agagcccgta cagttcgaag cagtcgctga ggccgtagtg
     2341 tccgagggcc ggtcccacga attcttccgc ctccgccagc ggaagcggtt cgagcaggcg
     2401 ctcccgggcg atgcggactg cctcggagag gcggggccac ttccattcgc cctcccggtc
     2461 cgtattcttg gggattttgc agaagtcggt gagtcctctc atcgaacacc accactttct
     2521 tccccgcacc gcttcgcggg ggaggaagga gagatcgaaa gagaggttgt ggacgacgat
     2581 tccctccggg ttccatcgct cccagaacgc ggcgaggctc ggccagtcct ccaggaaata
     2641 ggaaggatag cgttcttcct cgcgaaagtg cgcgatccgc tccggtgtga gtccgtggac
     2701 tcgctcggtc cgatgatcgg ggcgctcttc cgagtgatag aaacggttga aaaagtcgac
     2761 gatcctcccc tccgcggtga agacgatcga cgaggcggag acggtcgacg ctcccttgaa
     2821 gccgttcgtc tccaggtcga agacggcgaa gacgctgttg tttcccatga aataccctcc
     2881 ctgcacacgt ttcggacccc tccggggcga tcgcttttga acgctccgga gacgctgcta
     2941 ttatgacaga acagctcccg taagggaaag cgacgaaagg acgaaaagaa tgaacgacgc
     3001 gccattcaac gcattgagaa acgaggtcgt agcctgcgaa cgctgtcccc tcgccgagac
     3061 gcggttgcac gccgtttttg gggaaggccc ggaaagaacg ccggtcgtcc ttgtggggga
     3121 ggcccccgga gcgaaggagg acgaatccgg acggccgttc gtcggcctct cgggacgttt
     3181 gctgagcgaa ctgctggagg aggccgggct tcccagggag aagctgttca tcacgaacac
     3241 cgtgaagtgc cgccctcccg aaaaccgcct tccttcaaaa gcggagacgg ccgcctgccg
     3301 cccgtttctc gctgcgcagc tgcggcacat caggccgcgc ctcgtcatca ccgcgggaaa
     3361 cgcgcccacc cgcgcgctgc tggggacgaa agaggggata acgacgctgc gcggacgtct
     3421 tcacacctgc gtctgggagg gtatggagct ctccgttctg ccgctcttcc acccgagcta
     3481 cctccttcgg aaccggggac gcgccgaggg aagcccgatc atgctcacgc tggccgacat
     3541 ccgtgaagcg ctccgcctgg cgcggcagtt cacgcccgga ttttgacctc tcccacgaag
     3601 gggagaagcg cggttcggaa ctcctccaga aactccatgg gaaaggagga gagcgaggag
     3661 aagccgggat cgagcgtgtt ccccggacgg aaattctgaa ggacgaagag cggcgctcct
     3721 tcggccatac ggccgagaag gggcgcatcc tcgatacggt ggagccccgg aacgagcgtc
     3781 gtccggaact cgaaagggac ggccccgcgc agcagaaggg cgatgctctt ccgcacgagc
     3841 ggaaggagcg tcgcatcgcc gtcccggccg caggccaggg aatacgtttc ctccggagcc
     3901 ttgaggtcca tcgccgcgta gtcggccagc ttctccgcga ggatccgctc gaggacgtcc
     3961 gggcgcgccc cgttggtgtc gagcttcacc ttgagcccga gcgatcgtat ctccctcaga
     4021 aaggggagaa gcccgggctg aagcgccggc tctccgcccg tgacgcagac gccgtcgagg
     4081 aagggggctc gtttcttcaa gtccgcgagg atgtgttcct cggcgatccg ttcgtttgcc
     4141 gcccctggaa gaaccagctc cggattatgg caga
//
LOCUS       DAOPKU010000242         4145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23657_length_4145_cov_4.689976, whole genome shotgun sequence.
ACCESSION   DAOPKU010000242 DAOPKU010000000
VERSION     DAOPKU010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23657_length_4145_cov_4.689976"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PK849_08385"
     CDS             <1..772
                     /locus_tag="PK849_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE66180.1"
                     /translation="GSAAARTKTLHRNAPDAKPALVLDRVSGYGFRDISLSVYPGEVL
                     GIAGVVGAGRTELAEAVFGIGKLVGGRVLLEGADITGCGVRDAIRKGLNYIPEDRRRN
                     GLFGAASVRANVTSAVLDTLGRVFLPVFRERKLTSKYIEEFSISTTGQEQSLGALSGG
                     NQQKVIISRILATNPKVLILDEPTRGIDAGARGDVYRIIDALKAEGLAVLLISSDMEE
                     ILALSDRVVTMYNGAINREFGPGEIALDGLMAASFGVC"
     gene            774..1790
                     /locus_tag="PK849_08390"
     CDS             774..1790
                     /locus_tag="PK849_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66181.1"
                     /translation="MLPSFRMKREFNALLFAVLLFVGIGYVNPVFLTVQNIALTLQGS
                     VMYIFLAVGMTFVILTGDIDVSVGAVLGMSAAVAATLVRDGYPLWAVVGGALLVGGGT
                     GLLNGIGVTFLRIPAIVMTLGTMGILRGAMFIYTDGKWVENLPDFFKQACQICVCGIN
                     LFVLVAVALVLLMQIYLSTARRGKYFAAVGDNLDGAVLVGVPVVRIRIAAFIISGLCS
                     ALAGLVYTSQIGFVGNVAGNGTEMTAIAACVIGGVSLAGGTGSVAGALFGAVIMNTIN
                     SALVFLKVPAYWNNTISGLLLLTMVLADSLLHRYLEEKARRKRLNARFARAAERRACD
                     DARA"
     gene            1777..2799
                     /locus_tag="PK849_08395"
     CDS             1777..2799
                     /locus_tag="PK849_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="with IsrABC is involved with autoinducer 2 import;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 import system permease LsrD"
                     /protein_id="HPE66182.1"
                     /translation="MHVLKRYLLRWESVLILLLIAEIAIFGRINPRFLNTRVLLYSVN
                     DFVCVSIISLFMAFVVITGGIDISGGSIIGLTSVVVGVLWKQAGLDIVSASFAGLATG
                     ALCGAFNGFLVAFAGVQAMVVTLGGMMFFSGLALVLVGVSGVSAFEGISGFPDGFMEI
                     AGGDLWGVPNPALVFAALFAVACVLLHCTNYGRRVFLVGINPNAARYSGLGVRSVVMS
                     TYVLSGLGASIAGIVLTSYLGGARSDLGAELTLPIVTAVVLGGVSITGGRGTVVGVAI
                     AGVVVGLLRFGLQMAGVSSQYITVGTGMLLVVSAAVRGLAFRFSLLPRRSGGGDNIKG
                     GGARRG"
     gene            2910..3941
                     /gene="lsrB"
                     /locus_tag="PK849_08400"
     CDS             2910..3941
                     /gene="lsrB"
                     /locus_tag="PK849_08400"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF011937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HPE66183.1"
                     /translation="MKKFAVALAVVMLLVGAAAAVAAPKDVSVVFIPKLTGNMFFESA
                     AEGALAMAKEVGFECKYDGSPEASVANQVQIINSAVNQGFNAISVSSLSPTGLSQALQ
                     NAKKRGVKVVTWDSDVDPKDRAIYVNQGTPEILGAMLVDMAYDQMNDEQKKSAKFAFF
                     YSSPTVTDQNAWVDFAKKYIAEKHPGWEMLTVQYGEQDAQKSLQIGQSILQTYPDIDA
                     IICPDSTALPAMAQAAKNLEKAGKVIITGFATPSSMRGFVKDDTVKAFGLWDCKLQGA
                     IGAYIAYWLADGNTFKVGDFIDVPGVGKLKVEPNSIQGYDYEAEDSGIVLLPERVVFT
                     KENIDNYNF"
BASE COUNT          670 a         1332 c         1381 g          762 t
ORIGIN      
        1 ggggagcgcc gccgcacgga cgaagaccct ccaccggaac gccccggacg cgaagcccgc
       61 gctcgtgctc gaccgggtga gcgggtacgg cttccgcgac atttcgctct ccgtgtatcc
      121 cggcgaagtc ctcggaatcg cgggggtcgt cggggcgggc cggacggagc tggcggaggc
      181 ggtcttcggc atcgggaagc tcgtcggcgg cagggtgctg ctggaaggcg cggacattac
      241 ggggtgcggc gtccgcgacg ccatccgcaa ggggctgaac tacatcccgg aggaccgccg
      301 ccggaacgga cttttcggcg cggcgagcgt gcgggcgaac gtgacctcgg cggtgctcga
      361 cacgctcggg cgcgtctttc tgccggtctt ccgcgagcgg aagctgacct ccaagtatat
      421 agaagagttc tccatcagca ccaccggcca ggagcagagc ctcggcgccc tctccggagg
      481 gaaccagcag aaggtgatca tctcgcgcat cctcgccacg aaccccaagg tgctcatcct
      541 cgacgaaccc acccgcggca tcgacgccgg ggcgcgcggg gacgtctacc gcatcatcga
      601 cgcgctcaag gccgaggggc ttgccgtgct cctcatctcc tccgacatgg aggagatcct
      661 cgcgctgagc gaccgggtgg tcacgatgta caacggagcc atcaaccgcg aattcgggcc
      721 cggcgagatc gcgctcgacg gcctgatggc cgcctcgttc ggagtgtgct gacatgctgc
      781 cttcattcag aatgaaacgc gagttcaacg cgctgctctt cgcggtgctc ctcttcgtcg
      841 gcatagggta cgtcaacccg gttttcctca cggtgcagaa catcgcgctg acgctgcagg
      901 ggagcgtgat gtacatcttc ctcgcagtgg ggatgacctt cgtgatcctc accggcgaca
      961 tcgacgtgtc ggtcggggcc gtcctcggga tgtccgcagc ggtggctgcc acgctggtgc
     1021 gcgacggata tccgctgtgg gccgtcgtcg gcggggcgct gctcgtcggc ggcggaaccg
     1081 ggctgctgaa cggcatcggg gtcaccttcc tccgcattcc ggcgatcgtg atgacgctgg
     1141 gcacgatggg gattctgcgg ggcgctatgt tcatctatac cgacggcaag tgggtggaga
     1201 acctgcccga tttcttcaag caggcgtgcc agatctgtgt ctgcggaatc aatctgttcg
     1261 tcctcgtcgc ggtcgccctt gtgcttctca tgcagatcta cctctcgacg gcccgccggg
     1321 gcaagtactt cgccgccgtc ggcgacaatc tcgacggggc ggtgctcgtc ggcgtgccgg
     1381 tcgtgcggat ccgcatcgcc gccttcatca tctccggcct ctgctccgcc ctcgcgggac
     1441 tggtatacac cagccagata ggtttcgtgg gcaacgtcgc gggcaacggg acggagatga
     1501 cggcaatcgc ggcctgcgtc atcgggggcg tcagcctcgc cggggggacg gggtcggttg
     1561 ccggagcgct cttcggcgcg gtcattatga acacgatcaa ctccgcgctc gtcttcctca
     1621 aggtgccggc ctactggaac aacacgatct ccgggctgct gctgctgacg atggtgctcg
     1681 cggactccct gctgcaccgc tacctggagg agaaggcgcg gcgcaagcgg ctgaacgcgc
     1741 ggttcgcgcg cgccgcggag aggagggcgt gcgacgatgc acgtgcttaa acgatacctt
     1801 ctgcgctggg agagcgtgct catcctgctg ctgatcgcgg agatcgcgat cttcgggcgc
     1861 atcaaccccc gcttcctcaa cacgcgggtg ctgctgtaca gcgtcaacga cttcgtctgc
     1921 gtcagcatca tctcgctctt catggcgttc gtcgtcatca ccggagggat cgacatctcc
     1981 ggcggctcga tcatcgggct gacctcggtc gtcgtcggcg tcctctggaa gcaggcgggg
     2041 ctggacatcg tttcggcgtc gttcgccggg ctcgcgaccg gggcgctctg cggggcgttc
     2101 aacggctttc tggtggcctt cgcgggggtg caggcgatgg tggtcaccct cggcggcatg
     2161 atgttcttct cgggactcgc gctcgtgctc gtcggcgtgt cgggcgtcag cgcgttcgag
     2221 gggatcagcg gattcccgga cggcttcatg gagatcgcgg gcggcgacct gtggggcgtt
     2281 ccgaacccgg cgctcgtctt cgccgcgctc ttcgcggtcg catgcgtgct gctgcactgc
     2341 acgaactacg gccgccgcgt ctttctggta ggaatcaacc cgaacgcggc ccgctactcg
     2401 gggctcggcg tgcggagcgt cgtgatgagc acgtacgtcc tctccggtct cggcgcctcc
     2461 atcgccggaa tcgtgctcac ctcgtacctc ggcggagcgc gctccgacct gggcgcggag
     2521 ctgacgctgc ccatcgttac cgcggtcgtc ctcggcggcg tctccatcac ggggggcagg
     2581 gggacggtcg tcggcgtggc catcgcgggc gtcgtggtgg ggctgctgcg gttcggattg
     2641 cagatggccg gggtgtccag ccagtacatc accgtgggga cgggaatgct gctcgtcgtc
     2701 tcggcggcgg tcagagggct cgccttccgg ttttcactgc ttccccgccg ttccggcgga
     2761 ggggacaata tcaagggagg tggcgcccgg cgcggctgat gcggattggt tgatgcgcgg
     2821 tggattgagc ggttcggcgg agcgtcgccg gatcaggagg ggaatgaacc ggggttgcgg
     2881 ccccgaaaag aaaagaggag ggacttgtaa tgaagaaatt tgcggtagcg cttgcggtag
     2941 tgatgcttct ggtcggtgcg gcggctgcgg ttgcggcgcc gaaggatgtc agcgttgtct
     3001 ttattcccaa gctcaccgga aacatgttct tcgagtcggc ggccgagggc gcgctggcca
     3061 tggccaagga agtcggcttc gagtgcaagt acgacggctc ccccgaggcg tccgtcgcca
     3121 accaggtgca gatcatcaac agcgccgtga accagggctt caacgccatc tccgtctcct
     3181 cgctctcccc cacggggctg agccaggcgc tgcagaacgc gaagaagcgc ggcgtcaagg
     3241 tcgtgacgtg ggattccgac gtcgacccca aggaccgcgc catctacgtc aaccagggga
     3301 cgcccgagat cctcggcgcg atgctcgtcg acatggccta tgaccagatg aacgacgagc
     3361 agaagaagag cgcgaagttc gccttcttct actccagccc gaccgtcacc gaccagaacg
     3421 catgggtcga cttcgcgaag aagtacatcg ccgagaagca ccccggctgg gagatgctga
     3481 cagttcagta cggcgagcag gacgcgcaga agtcccttca gatcggccag tccatccttc
     3541 agacctaccc cgacatcgac gcgatcatct gccccgactc caccgctctt cccgcgatgg
     3601 cgcaggccgc caagaacctt gagaaggccg gaaaggtcat catcaccgga ttcgccaccc
     3661 ccagctcgat gcgcggcttc gtgaaggacg acaccgtcaa ggcgttcggc ctgtgggact
     3721 gcaagctcca gggcgcgatc ggcgcgtaca tcgcctactg gctggccgac ggcaacacct
     3781 tcaaggtcgg cgacttcatc gacgttcccg gcgtgggcaa gctgaaggtc gagcccaact
     3841 ccatccaggg gtacgactac gaggccgagg actccggcat cgtcctgctc cccgagcggg
     3901 tcgtcttcac caaagagaac atcgacaact acaacttcta accatcgctg cacggcttcg
     3961 ccacgcgctc ccggcgcctc gggggcgcgt ttttcatacc cggcaagccc caagcgctcc
     4021 cgggccgccg ccttaccgtc tgctcttgcg tcccggctcc ggaaatgtca tactcaattg
     4081 gtacaagtat attttttctc gggagggaat actgaatgaa gcctacagca ctctcctcgt
     4141 cgcgg
//
LOCUS       DAOPKU010000243         4142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23686_length_4142_cov_4.214093, whole genome shotgun sequence.
ACCESSION   DAOPKU010000243 DAOPKU010000000
VERSION     DAOPKU010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23686_length_4142_cov_4.214093"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PK849_08405"
     CDS             <1..282
                     /locus_tag="PK849_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HPE66184.1"
                     /translation="TGLQDSSPTQRPWVGEVSCNPVEQARRLDEAGSQFNIVLGLCVG
                     HDSLFYRHSKAPVTTFVVKDRVLGHNPVAVLYSQYLRKNLKKRPEERLK"
     gene            458..2980
                     /locus_tag="PK849_08410"
     CDS             458..2980
                     /locus_tag="PK849_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66185.1"
                     /translation="MRKRAAALSMQTALALAFTLFVLLSAGIAGYLSQRSGRESVEEI
                     AARLRGDMATHIGEHVRHFLHIPHIINRLNADALGGGMLDVLDERSLEFHFRKQIDVF
                     PSVSSIYFGNTSGGLVNSGREAPFDSRYVIGTIGSKAGVFRKYSIDKDGNRTGLLAEV
                     PDFDARTRGWYSRAQKSGGEGVWSDVYVLFTGQDMAITASRPVYDAHGGFLGVAAVDL
                     SLSQLTAFLQALPLGEGTAFIMERSGLMLASSRGEPPFVFYEGGEARRLHAADSVHAE
                     ISGAALFMASELEKNGLLTDGEIAFESGGERCYLRTESLHGPGDLDWIIAVTAPEREF
                     MDRIDKNNRTTTLVILFAVLLSFGAALLLARRMAQPVLRLNAAARAITRGENPPELPV
                     DCEILEVGELSASFNRMAEALRETIRGLNEEIGVRKETESRVRAAMERAEDLRKEAEN
                     ANRAKSEFLANMSHEIRTPLNGVVGFLDMLRGPGLDQEQLSYVDNAKVSAYALLEIIS
                     NILDISKIEAGKLELETVRTDIPKLLEESLVIAGHGSVSRGVRVFSSVHPPFPRSAYV
                     DPLRLRQVLVNLLGNAVKFTEEGEVELSASFQPSAGAEHMGLFTFSVRDTGIGIRKED
                     REKLFRPFSQADASNTRKYGGTGLGLVISNGILKKMGSALELESEPGNGSRFSFTVSA
                     RYEEDVSLPDTPRESKGEVRQEQAFSELSSRAFSLLIVEDLRMNRTLLRLLASRLLPS
                     AAVFEAENGERAVEMFHTHAPDIVFMDMHMPVMNGFEATAAIRELEKARGTHTPIVAL
                     TADVLSETRERCFQAGMDDFLPKPIEAESLRAVLERYLSLSF"
     gene            3084..3779
                     /locus_tag="PK849_08415"
     CDS             3084..3779
                     /locus_tag="PK849_08415"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPE66186.1"
                     /translation="MGPIKRVRLSERVTDAIETMIAEGAFAPGDRFLSENDLVKKLEV
                     SRSSVREAVRILEVRGRVIVKHGKGIFIAEPPDEDRDPFTGWLKDNKAPIFDHFEVRL
                     IIEPKAARRAAEKADDESVKGIEKAYGAFVASAERGDTAAMIRFDGEFHRLVAKATKN
                     RTLYHLMKTMTKSLPEGWITSLHIPGRVEKTLGEHSSILDAIRGGDPERAEEMMTLHL
                     ANALKDIQASMER"
     gene            3800..>4142
                     /locus_tag="PK849_08420"
     CDS             3800..>4142
                     /locus_tag="PK849_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE66187.1"
                     /translation="MTIITTLIENSPGEHHGLWSEHGISFHVQKDGHSLLFDAGQSEA
                     FIHNAARLRVNLSATDFVVLSHGHYDHSGGFRCLCDYASDFELRIGKGFFEDKYAVRG
                     AAHDFLGNDFDE"
BASE COUNT          930 a         1107 c         1227 g          878 t
ORIGIN      
        1 acaggattgc aggactcctc cccaactcag cgcccctggg ttggggaggt gtcctgcaat
       61 cctgtcgaac aggcccgccg gctcgacgaa gcgggaagtc agttcaacat cgtcctcggc
      121 ctctgcgtgg gacacgacag cctcttttac cgtcactcga aggcgcctgt cacgaccttc
      181 gtcgtcaagg accgcgtgct cggacacaac ccggtagcgg ttctttacag tcagtacctc
      241 aggaaaaatt tgaagaaacg tcccgaggag aggttgaagt agagcggaac aaagggaggc
      301 cgaggagaat gcggcctccc tttttcttcg gtacctcatt ttttaggtgt gaccattcag
      361 gaaaatggcg cgtgcggatg gccgtgaggg gcgtcttgtt ttataatgat tcaggacgta
      421 tgacgtcgta cgtaagggtt gatagtgatt gaagagaatg agaaaaagag cggctgcgct
      481 ctcgatgcag acagctctcg cgcttgcttt cacgcttttt gttctgcttt cagcgggtat
      541 tgccgggtat ctgtcgcaga gaagcggacg ggaatcggta gaggaaatag ccgccaggct
      601 gcgaggcgat atggcgacgc atatcggcga gcacgtgcgt cattttctcc acatacctca
      661 tattatcaac cgcctgaacg cggatgcttt gggcggggga atgctggatg ttctcgacga
      721 gcgctcgctg gaattccatt tcaggaagca gatagatgtt ttcccttcag tttcgagcat
      781 ctacttcggc aatacgtcgg gaggactcgt caacagcggc agagaagctc cttttgattc
      841 ccgctacgtc atcggcacga tcggtagcaa agcgggcgtc ttccggaagt actccatcga
      901 caaagacggc aaccgcaccg ggcttttagc cgaagtcccc gattttgacg cgcggacgag
      961 aggatggtat tcccgtgctc aaaagagcgg aggcgaggga gtctggagcg acgtgtatgt
     1021 tctctttaca ggtcaggata tggccattac cgccagtcgc ccggtgtacg acgctcatgg
     1081 aggttttctc ggcgtagcgg ccgtggacct ctccctttcg cagctgacgg catttttgca
     1141 ggcgctgccg ctcggcgagg gaaccgcgtt catcatggag cgctcgggac tgatgcttgc
     1201 ctcttcgaga ggggaacctc catttgtctt ctacgaagga ggagaagccc ggcgcctgca
     1261 tgccgccgac agcgtgcatg ccgaaatcag tggagccgct cttttcatgg cgagcgaact
     1321 cgaaaagaac ggtttgctca cagacgggga gatcgctttt gaaagcggcg gggaacgctg
     1381 ctatcttcgc acagaatccc tgcacggccc gggagatctt gactggatca tcgccgttac
     1441 ggctccggag agagagttca tggaccggat tgataaaaac aaccgcacga cgacgctggt
     1501 tatcctcttt gccgtgctcc tctctttcgg cgcggcgctg cttcttgccc gaagaatggc
     1561 gcagccggtc cttcgcctga atgccgccgc gcgtgcgatc accagagggg agaatccgcc
     1621 cgagcttcct gtcgactgcg aaattctcga ggtgggcgag ctttccgcgt cgttcaacag
     1681 gatggcggag gcgctgcgtg aaaccatccg cggcttgaac gaagagatcg gcgtgcgaaa
     1741 agagacggaa tcccgtgtgc gcgccgcgat ggaaagggcg gaagacctca gaaaagaagc
     1801 ggagaacgcg aacagggcga aaagcgaatt tctggcgaac atgagccatg agatcagaac
     1861 gccgctcaac ggggttgtcg gctttctgga tatgttgcgc ggcccggggc tcgaccagga
     1921 acagctctct tacgtcgaca acgcgaaagt gtccgcctac gcgctcctgg aaataataag
     1981 caacattctc gatatttcta aaatagaggc cggtaaactt gagctggaga ccgttcggac
     2041 ggatattccc aagctgctcg aagaatctct ggtcatcgca ggtcacgggt cggtaagcag
     2101 aggcgttcga gtcttctcga gcgttcaccc tcccttcccg cgctctgcgt atgtggatcc
     2161 cttgcgtttg cggcaagtgc ttgtcaatct tctggggaac gccgtcaaat tcacggagga
     2221 gggagaagtc gaactctccg cctcttttca gccctcagcc ggcgctgaac acatggggct
     2281 tttcaccttt tccgtgcgtg ataccggaat aggcatccga aaagaagaca gagagaagct
     2341 cttccgtccg ttttcccagg cggacgcgtc gaacacgaga aagtacggcg gaacgggact
     2401 cggtcttgtt atctccaatg gtattctaaa aaaaatggga agcgctctcg aacttgagag
     2461 cgagcccggg aatggaagcc gcttttcttt cacggtttcc gcacggtacg aggaggacgt
     2521 ctctttgccg gacaccccgc gggagtcgaa gggtgaagtg cgacaagagc aggcgttttc
     2581 cgagctttct tcacgagcct tctcgctcct catagtggaa gatctcagaa tgaacaggac
     2641 gctcctgagg ctgcttgcgt cgcgattgct cccctccgcc gctgtttttg aggcggaaaa
     2701 cggggagcgg gccgtggaga tgttccatac gcacgcgccg gacatcgtct tcatggatat
     2761 gcacatgccg gtgatgaacg gcttcgaggc gaccgccgcc atcagagagc ttgagaaagc
     2821 ccgcgggacg cacaccccga tagtcgccct gacggcggat gtgctgtccg aaactcgcga
     2881 acgctgtttt caggcgggta tggacgactt tcttccaaaa ccgatagagg ctgaatcgct
     2941 gcgcgccgtc ctggagcggt atctttcttt gtctttctga actttttact ctcttgacag
     3001 ggtgcggaaa acaggggtac aatcaacttg tctgataaga agatgtccgc tatgttctca
     3061 tcttgcgaaa agcgggtgaa actatgggcc ccataaaaag agtacgcctg tccgaaaggg
     3121 taacagacgc gattgagacg atgatcgcag agggggcttt cgcccccggg gacagattcc
     3181 tttccgagaa cgatcttgta aaaaaacttg aggtgagccg ttcctccgtc cgtgaggccg
     3241 tccgaattct ggaggttcgc ggaagggtga tcgtgaagca cggcaagggt atttttattg
     3301 ccgaacctcc ggacgaggat cgcgacccct tcacaggatg gctgaaggac aacaaggccc
     3361 cgatttttga tcatttcgag gtgcgcctga tcatagaacc caaagccgcg cggcgcgccg
     3421 ctgaaaaagc cgacgacgaa agcgtcaaag gaatcgaaaa ggcctacggg gcattcgtcg
     3481 cgagcgcgga gcggggagac accgcggcaa tgatacgttt cgacggagag tttcacagac
     3541 tcgtcgcaaa agctacgaag aaccggacgc tctatcacct gatgaaaacg atgacaaaat
     3601 ctcttcccga aggatggatc accagcctcc atattccggg aagagttgaa aaaacacttg
     3661 gagaacactc gtccatcctt gatgcgatca gggggggcga ccccgagcgg gcggaggaaa
     3721 tgatgacgtt gcacctcgcc aacgccctca aggatatcca ggcatccatg gagcgctgaa
     3781 agcgcgaagg gggagcgtca tgacgatcat caccacgctg atagaaaact cgccgggcga
     3841 gcatcacgga ctttggtccg agcacggtat ctccttccac gtacaaaaag acgggcactc
     3901 ccttctcttc gacgctgggc agtccgaggc gtttatccat aacgcggcaa ggctgcgcgt
     3961 caacctctcg gcaacggatt tcgtcgttct tagccacggt cattacgacc actccggcgg
     4021 tttccgttgt ctttgcgatt acgcttcgga tttcgagctg cgtataggaa agggtttttt
     4081 cgaggacaaa tacgccgtcc ggggagccgc ccacgatttt ctgggtaatg acttcgacga
     4141 ga
//
LOCUS       DAOPKU010000244         4133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23741_length_4133_cov_4.089505, whole genome shotgun sequence.
ACCESSION   DAOPKU010000244 DAOPKU010000000
VERSION     DAOPKU010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23741_length_4133_cov_4.089505"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PK849_08425"
     CDS             complement(<1..274)
                     /locus_tag="PK849_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE66188.1"
                     /translation="MITRMLFQLLRQIRQKVFTNPFPVAQMPDSLTDALQAAEKGWIE
                     LNPPVGVNDHFRGRLNYDKSACIGCKLCIKVCPANATEYLPEEKKIQ"
     gene            complement(291..1283)
                     /locus_tag="PK849_08430"
     CDS             complement(291..1283)
                     /locus_tag="PK849_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPE66189.1"
                     /translation="MIALLMRLVAGTALMLLVVVLALLFDGADRIISARMQRRLGPPL
                     LQPFLDLLKLLGKENIVPRRAVPWVFNGAPWVAAASMLLVFLYIPVGSVPPVLGVHGD
                     LILILYLLGLSGVAMAVGGFASGNPIANVGAQREMILMMSYELPLAVVASTLAWVAYK
                     TGMPGDPFNLETFAGMSVWGMSGKIGFIGIFCLMVALILVVPGETGKGPMDIPEAKTE
                     ILDGLIIEYSGRNLAMFKITFALRAMALSALITALFFPWSPASFLRLGGIFLFTFDFV
                     WFWVKVFVVQIFVVTLMRTTFGRFKIWQASEFYLVKVAGLSIAGMILASLGLVV"
     gene            complement(1280..2503)
                     /locus_tag="PK849_08435"
     CDS             complement(1280..2503)
                     /locus_tag="PK849_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPE66190.1"
                     /translation="MEKKYTIPIGPVHVGLKEPITAWLDLEGEKIVGATIRPGAIHRG
                     IEFMARDRNPIQVIYLSERICGICSFAHVIAFLRAVEDAAEYPVPVRAQYIRTLVLEL
                     ERLHSHILWAGVACYSIGFDSAFHLGMVLREKVMDVLEALSGNRVNYGVATVGGVRWD
                     ITPEVAKAVREMVKYYKAEFGAFYDVVTADPIAKARMRNVGVLTPDEAVRYNAVGPTA
                     RASGIRCDIRETSPYEAYADFNVKAIVPQDYYGKAYGDVFDRFLVRVLEVQQSLNIVE
                     RVMDGLPEGEIVHEKNLNKVLATLKKAEGTGWGVIEAPRGDDTHVVHLKAGEENVNWW
                     KVRAPTYANAVSWPLMFRDNELADAPLIINSIDPCISCMERMLVTDAAGGRSVVTKAE
                     LLEKSREKTRRLMQK"
     gene            complement(2516..3067)
                     /locus_tag="PK849_08440"
     CDS             complement(2516..3067)
                     /locus_tag="PK849_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPE66191.1"
                     /translation="MTFPVKGLNAETGREPEELLARIRELFGEGAVVNVRAHTAGLKE
                     EAQYRDIWLSVEKGRFLEMIDVLAEFDFPMFHVMSGNDDGDVVTLNYHFGIFQCAGRG
                     KRFGVAVSVALPKNDLVIPSLFSRIPGVEYSEREMREMLGITFDGLPNTALVFLPEDW
                     DEAVKPWRRDETGPKPENVRELS"
     gene            complement(3064..3534)
                     /locus_tag="PK849_08445"
     CDS             complement(3064..3534)
                     /locus_tag="PK849_08445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPE66192.1"
                     /translation="MNTTLLEKYLDILPKSLWVTTCNSGSCNGCDIEIVATISPRYDI
                     ERFGMKLVGTPRHADVLIVTGPVTKYMKDRLLRIYNQIPDPKAVVVVGNCGASGDVFY
                     KSYNLDGPVDNIIPVDVYVHGCPPRPEAIIEGVAKAVLKLESLKQQKKAGEADS"
     gene            complement(3547..3885)
                     /locus_tag="PK849_08450"
     CDS             complement(3547..3885)
                     /locus_tag="PK849_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HPE66193.1"
                     /translation="MFGTVYTGFGYWDVSAWIVFFAIAAGLTLWLRAQGREDFKEGTD
                     QDENYWSGNPVPDDGAAISVPASSAYWGFRKAMEPFYKVLTGMHTGIGTDIVGFYVLT
                     VAFMAVFILL"
     gene            complement(3885..>4133)
                     /locus_tag="PK849_08455"
     CDS             complement(3885..>4133)
                     /locus_tag="PK849_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HPE66194.1"
                     /translation="SVLTLASFVKVFQTAFLGPEKSVFTGVREVPSGMLFGMGVLTLA
                     ILGASLFPSWTLSNLVEPAAKALVDQAGYIGAVMGGGL"
BASE COUNT          878 a         1302 c         1167 g          786 t
ORIGIN      
        1 tctgaatctt cttctcttcg ggcagatact ccgtcgcatt cgccgggcag accttgatac
       61 agagcttgca ccctatgcat gcgctcttgt cgtagttcag acggcctcgg aagtgatcgt
      121 tcacaccaac gggagggttc agttcgatcc accctttttc ggcggcctgc agcgcgtcgg
      181 tcagcgagtc gggcatctgc gccacgggga aggggttcgt gaacaccttc tggcgaatct
      241 gccgcagcag ttgaaagagc attctggtga tcattccgtt ccaccccttc ctatacgacc
      301 aggccgaggg acgcgaggat catccccgcg atggaaagcc ccgcgacctt gacgagatag
      361 aactcggacg cctgccagat tttgaacctg ccgaacgtcg tccgcatcag cgtcaccacg
      421 aagatctgca ccacgaagac cttcacccag aaccagacga agtcgaatgt aaagaggaag
      481 atgcctccca ggcgaaggaa ggatgcggga gaccacggga agaagagcgc cgtgatcagt
      541 gcggagagcg ccatggcgcg cagcgcgaag gtgattttga acatggcgag gtttcttccg
      601 gagtactcga tgataagacc gtcgagaatc tccgtcttcg cttcggggat gtccatcggc
      661 cccttcccgg tctctccggg aacgacgagg ataagagcga ccatgagaca aaaaataccg
      721 atgaagccta tcttgccgct cattccccag accgacatcc cggcaaaggt ctccaggttg
      781 aacggatctc cgggcattcc cgtcttgtag gcaacccacg caagcgtgga ggcaacgacg
      841 gcgagcggaa gttcgtagct catcatcagg atcatttcac gctgcgcgcc cacattcgct
      901 atcgggtttc cgctggcgaa gccgcccact gccattgcaa cgccggagag ccccaggaga
      961 tagagaatca ggatcaaatc tccgtgcacc ccgagcacgg gaggcaccga gccgaccggg
     1021 atgtagagga aaacgaggag catgctcgct gccgcaaccc agggcgcgcc gttgaaaacc
     1081 cacggaactg ccctccgcgg cacgatgttc tccttgccga ggagcttcag caggtcgaga
     1141 aaaggctgca gaagaggcgg cccgagacgg cgctgcatgc gggcgctgat gattctgtcc
     1201 gctccgtcga aaagaagcgc cagcacgacg acaagaagca taagcgctgt accggcaacg
     1261 aggcgcataa gaagcgcgat catttctgca tcagcctcct tgttttttcc ctgctctttt
     1321 ccaggagctc cgccttggtg acgacgctcc tgccgccggc ggcgtccgtg acgagcatac
     1381 gctccatgca cgagatgcag gggtcgatgc tgttgataat aagcggcgcg tcggccagct
     1441 cgttgtcgcg aaacatcaac ggccatgaaa cggcgttcgc gtacgtcggg gcgcgcacct
     1501 tccaccagtt cacgttctcc tcgccggctt tcagatgcac cacgtgcgtg tcgtcgccac
     1561 gcggagcctc gatgacgccc caccccgtgc cttcggcctt cttgagcgtc gcgagcacct
     1621 tgttcaggtt cttctcgtgg acgatctcgc cctcgggaag accgtccatc acccgctcga
     1681 cgatattcag cgactgctga acctccagca cgcgtacaag gaaacggtcg aacacgtcgc
     1741 cgtacgcctt gccgtagtag tcctgcggaa cgatcgcctt cacgttgaaa tcggcgtacg
     1801 cttcgtacgg cgacgtctcg cggatatcgc agcggattcc gctcgcgcgg gcggtcggcc
     1861 ccaccgcgtt gtaccggaca gcctcgtccg gcgtgagcac gccgacgttg cgcatgcgcg
     1921 ccttagcgat aggatccgcc gtgacgacat cgtagaacgc gccgaattcc gccttgtagt
     1981 atttcaccat ttccctgacg gccttcgcta cctcgggcgt gatatcccac cgcacgccgc
     2041 cgactgtggc gacgccgtag ttcaccctgt ttccggagag cgcctcaagc acgtccatga
     2101 ctttctcgcg gagcaccatc ccaaggtgga aggcggagtc gaagcctatg gaatagcagg
     2161 cgacgccggc ccacagaatg tgggagtgca gccgttcgag ctccagaacg agcgtccgga
     2221 tgtactgcgc gcgcacaggc acagggtatt ccgccgcatc ctcgacagcg cggagaaacg
     2281 cgatcacgtg cgcgaaggag cagatgccgc aaatcctttc cgacagatag atcacctgga
     2341 tgggattccg gtcgcgcgcc atgaactcga taccgcgatg aatcgctccc ggccggatag
     2401 tggcgccgac aattttttcg ccttcgaggt ccagccacgc agtgatgggc tccttcaagc
     2461 caacatgaac gggacctata ggaatcgtat atttcttctc catcagtcgt tcctcctaag
     2521 aaagttcccg tacattttcg ggcttcggcc ccgtctcgtc cctgcgccac ggcttgacgg
     2581 cctcgtccca gtcctccggg aggaagacga gcgccgtgtt cggaagcccg tcgaacgtga
     2641 taccgagcat ttcacgcatt tcgcgctcgc tgtactcgac gcccggaata cgggaaaaaa
     2701 gcgacggaat gacaaggtcg ttcttcggaa gcgccacgct cacggccacg ccgaagcgtt
     2761 tgccgcggcc ggcgcactgg aagatcccga aatgatagtt cagcgtgacc acatcgccgt
     2821 cgtcgtttcc ggacatgacg tggaacatcg gaaaatcgaa ctccgcaagc acgtcgatca
     2881 tttcgagaaa gcgccccttc tccaccgaca gccagatgtc gcgatactgc gcctcttctt
     2941 taagccctgc ggtatgggcg cgcacattga cgacagcgcc ctcgccgaag agttcgcgaa
     3001 tacgggcaag aagttcttcc ggctcccgcc ccgtttccgc gttgagtcct ttcaccggaa
     3061 aagtcatgag tccgcctcac ccgctttctt ctgttgcttc aatgattcca gcttcagcac
     3121 cgccttggcg acgccttcga tgatcgcctc ggggcgcgga gggcatccgt ggacatagac
     3181 gtccacgggg atgatattgt ccacagggcc gtcgaggttg tacgacttgt agaagacgtc
     3241 tcccgacgcg ccgcagttcc ccaccaccac gaccgccttc ggatcgggta tctggttgta
     3301 gatgcgcagc agcctgtcct tcatatattt cgtcacaggg ccggtcacaa tgagcacatc
     3361 ggcgtggcgc ggtgttccca caagcttcat gccgaaacgt tcgatgtcgt agcgcgggct
     3421 gatagtggcc acgatttcaa tatcgcatcc gttgcacgac ccggagttac aggtcgtcac
     3481 ccagagcgat ttgggcagaa tatccagata tttctcaagc agcgtagtgt tcatggcgcc
     3541 ctcctcctac agaagaatga acacggccat aaaggcgaca gtcagaacgt aaaacccgac
     3601 aatatccgtc cctattcccg tgtgcattcc tgtcagcacc ttgtagaaag gctccatggc
     3661 ttttctgaaa ccccaatacg cggaagaggc gggaacggat atcgccgccc cgtcgtcggg
     3721 cacggggttg ccggaccaat agttctcgtc ctgatccgtc ccctccttaa agtcctcgcg
     3781 cccctgggcg cggagccaga gcgtcagacc ggcggcgatg gcgaagaaga cgatccacgc
     3841 ggagacgtcc cagtagccga atccagtgta gacagtgccg aacatcagag cccacctccc
     3901 ataacagcgc cgatataccc ggcctgatcg accagggcct tcgccgcggg ctcgacaagg
     3961 ttcgacaagg tccacgacgg gaagagcgaa gcgccaagaa tcgcgagcgt cagcactccc
     4021 atgccgaaca gcattcccga cggaacctcc ctgacgcctg tgaagacgct cttttcggga
     4081 ccgaggaagg ccgtctgaaa gacttttaca aaggaagcca gcgtcagcac cga
//
LOCUS       DAOPKU010000245         4128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23778_length_4128_cov_5.205254, whole genome shotgun sequence.
ACCESSION   DAOPKU010000245 DAOPKU010000000
VERSION     DAOPKU010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23778_length_4128_cov_5.205254"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2437
                     /locus_tag="PK849_08460"
     CDS             <1..2437
                     /locus_tag="PK849_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPE66195.1"
                     /translation="GSHKKSARVVGETMGKYHPHGDSSIYEAMARMAQNFSLRVPLVD
                     GQGNFGSLDGDSPAAMRYTEVRLTPEGELMHADVDEETVNFVPNFDESLTEPSVLPAV
                     LPNLLINGASGIAVGMATNIPPHNPVETLSLLSEYLRDGCSWSVSETASRMPGPDFPT
                     GGEIVGTTGIREMYETGRGKFTLRGKTHIEETKNRYLVVVTEIPYGATKSRIVAQIAE
                     KLDEKNVEGIVTVRDESDRTGDRVVVEMQKKTDPKAVEALMHSATQLSGTFGGNLLAL
                     AGGAPRTMTLKDIFDCFVGHRREIVRRRTEFRLKRDEARLHIVEGLLKALDILDEIVA
                     LIRASKSVAEAKAGLVDRFGFSVLQAEAILEMRLSRLVGLEYEALVKEKKKLEKAIAE
                     WKGILASAKKLDSVVAGELDELAARFAKSPLAARRTSILDEEPEKKRGKDAPGQARLS
                     FEQPSPCVISLDAEGYIRKREMKRRPRDEEEGSVFVTNGIVYALGDDGRFYSRERAGI
                     PDASTKKLLPAQEFFGAGEGVSLVLVSPDDHDEALFVFADGSVKRASLDTLKGDTSRK
                     NTSKTATPLNEGEKLLAVVPLKGDGDFDIMLCTKSGRAIRIPGAKIPLKGTAARGVAG
                     IAVEDEDALVSAVALPRSEAAPDDLFAVVRTSNGRFFRFALSELKSSGRGGKGIYIHR
                     GDKPSFGEVAEIAALSPGDSLIADDGAEIPAENVRPRDVGEHTLVSMTEIPGMSRLSV
                     RRRKNGRNGNGGTNGDEGADESVPEQDAGGAPARRLFAENDEGVQISGGSGDFGEEED
                     FAGFPEDDGGEER"
     gene            2434..>4128
                     /locus_tag="PK849_08465"
     CDS             2434..>4128
                     /locus_tag="PK849_08465"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HPE66196.1"
                     /translation="MSGFNYDGSSIRVLEGLAAVRMRPGMYIGDTGQRGLHHLIWEIV
                     DNSVDEALAGYCTEISVALAPDGSVLVADNGRGMPVDPHPQKRVPTCQVIMTVLHAGG
                     KFGDGAYTVSGGLHGVGASVVNALSEKLELFVRRDGREYSMTFMRGDVFKELSSKPAK
                     TPGTGTTVRFYPDPLIFKTGIVFDPKLVERRLREISYLVPGLSLTLETVNAEGETVRE
                     TFMNKDGIEAMTNRLAKNTTPLFEGTVAFTGTSGDTEVAFALKYLDDYSERMYSFVNL
                     IPTAEGGTHVAGFRAALTRAVNEAAKTEKLLKAGEGNILGDDLREGLLCVLSVKLPNP
                     QFEGQTKGKLGNGETTGAVSTLVYDKLSYFFANEGGGILRIIVDKALSTRRAREAAKR
                     AKELVRSKGGGGRDAGLPGKLADCISRKPAEAELFIVEGDSAGGSAKQGRDRKFQAIL
                     PLRGKILNVEKASLDKILANAEVGNIVKALGCGVGSEFDVSRLRYGKIVVMTDADVDG
                     AHIRTLLLTLFYRLLPQLVSEGRIYAAQPPLYRAAKGKDVRWLYSDAELREFVGRGK"
BASE COUNT          785 a         1246 c         1460 g          637 t
ORIGIN      
        1 gggatcgcac aaaaagtcgg cgagggtcgt cggcgagacg atgggcaagt accatccgca
       61 cggcgactcc tcgatatacg aggccatggc gcgcatggcg cagaacttct cgctccgcgt
      121 cccgctcgtg gacggacagg gaaacttcgg ctcgctcgac ggcgactctc ccgcggcgat
      181 gcgctacaca gaggtccgcc tgacgccaga gggagagctg atgcacgccg atgtcgacga
      241 ggagacggtg aacttcgtcc cgaacttcga cgagtcgctc accgagccct ccgtccttcc
      301 cgccgttctt ccgaacctgc tgatcaacgg agcgtcggga atagcggtcg gcatggcgac
      361 gaacatcccg ccgcacaatc ccgtcgagac gctctccctc ctctcggagt accttcggga
      421 cggctgctcg tggagcgtct ccgagacggc gtcgaggatg cccgggcccg acttccccac
      481 ggggggcgag atcgtcggga cgaccggtat ccgcgagatg tacgagacgg ggcgcggcaa
      541 gttcacgctt cgcggcaaga cgcatataga agaaacgaag aaccgctacc tcgtcgtggt
      601 caccgaaatt ccgtacggcg ccacgaagtc gcgcatcgtc gcgcagatcg ccgagaagct
      661 cgacgagaag aacgtcgagg ggatcgtgac cgtccgggac gagtccgaca ggacgggcga
      721 ccgggtggtc gtggagatgc agaagaaaac cgatcccaag gccgtggagg cgctgatgca
      781 ctcggccacg cagctctccg gcaccttcgg cggcaacctg ctcgcgctcg cggggggcgc
      841 gccgcggacg atgacgctca aggatatctt cgactgcttc gtcggccacc ggcgcgagat
      901 cgtccggcga aggaccgagt tccgtctgaa gcgcgacgag gcgaggctgc acatcgtcga
      961 ggggctgctg aaggcgctcg atattctcga cgagatcgtc gcgctgatac gcgcctcgaa
     1021 gagcgtcgcc gaagcgaagg ccgggcttgt cgatcgattc ggcttctcgg ttctccaggc
     1081 tgaggcgatc ctcgagatgc gcctttcccg tctcgtcggg ctggagtacg aggcgctcgt
     1141 caaagagaag aagaagctgg agaaggccat cgcggagtgg aagggaattc tcgcctccgc
     1201 gaagaagctg gactccgttg tggccggtga gttggacgag ctggcggcgc gtttcgcgaa
     1261 gagcccgctc gcagcgcggc ggacctccat tctcgacgag gagccggaga agaagcgcgg
     1321 caaggacgcg ccggggcagg cgcgactctc gttcgagcag ccgtcgccct gcgtgatctc
     1381 gctcgacgcc gaggggtaca tccggaagcg cgagatgaag cgcaggccgc gcgacgagga
     1441 ggaggggagc gtcttcgtga cgaacggcat cgtctacgcg ctcggcgacg acgggcgctt
     1501 ctactcgcgc gagcgcgcgg gcattcccga cgcctcgacg aagaagcttc ttccggcgca
     1561 ggagttcttc ggagccggtg agggagtgtc gctcgttctc gtctcccccg acgaccacga
     1621 cgaagcgctc ttcgtcttcg ccgacggctc cgttaaacgg gcctctctcg atacgctcaa
     1681 gggcgatacg tcgaggaaaa atacttcgaa aaccgcgacc ccgctgaacg agggggagaa
     1741 gctgcttgcg gtcgttccgc tcaaggggga tggagacttc gatatcatgc tctgcacgaa
     1801 gtccggaagg gcgatccgta tccccggggc gaagatccct ctgaagggaa cggcggcgcg
     1861 gggcgtcgcc ggaatcgcgg tggaggacga ggacgcgctt gtttcggcgg tcgcgcttcc
     1921 gaggagcgag gccgcgcccg acgacctgtt cgcggtggtg cggacgtcga acggcagatt
     1981 cttccgcttc gcgctctcgg agctgaaatc ttccgggagg ggaggcaagg gaatctatat
     2041 ccacaggggg gacaagccct ccttcggcga ggttgccgag atcgcggcgc tttcgccggg
     2101 cgactcgctg atcgcggacg acggggcgga gattcccgcg gagaacgtcc gcccccgcga
     2161 tgtgggcgag cacacgctcg tctccatgac ggagattccc ggtatgtcgc gcctgtctgt
     2221 caggcgcagg aagaacggac gtaacggaaa cggcggaacg aacggcgacg aaggcgcgga
     2281 cgagagcgtc cccgaacagg acgcgggcgg cgctccggcc cggaggctct ttgcggagaa
     2341 cgacgaggga gtgcagatca gcggcggcag cggggacttc ggcgaagaag aggatttcgc
     2401 cggtttcccc gaggatgacg gaggagaaga acgatgagcg gattcaacta cgacggcagt
     2461 tccatacggg tgctcgaagg gcttgcggcg gtgcggatgc gccccggaat gtacatcggc
     2521 gacacggggc agcgagggct gcaccatctg atctgggaga ttgtggacaa ctccgtcgac
     2581 gaagcgctcg cgggctactg caccgagatt tcggtcgcgc tcgcccctga cgggagcgtc
     2641 ctcgtcgccg acaacgggcg tggtatgccc gtcgacccgc acccgcagaa gcgcgttccc
     2701 acctgccagg tcatcatgac cgtcctccac gcgggaggca agttcggcga cggcgcgtac
     2761 accgtctcgg gcggccttca cggggtgggc gcgtcggtgg tgaacgccct ctccgagaag
     2821 ctggaactct tcgtgcggcg ggacggccgg gagtactcca tgaccttcat gcgcggcgac
     2881 gttttcaagg agctttcgtc gaagccggcg aagacccccg gtaccggcac gacggtgcgg
     2941 ttttaccccg atccgttgat cttcaagacg gggatcgtct tcgatccgaa gctcgtcgaa
     3001 cgccgcctgc gcgagatctc ctacctcgtg ccggggctct cgctgacgct ggagacggtg
     3061 aacgcggagg gggagacggt ccgggagacc ttcatgaaca aagacggcat cgaggcgatg
     3121 acgaaccgcc tcgcgaagaa caccacgccg ctcttcgagg ggacggtcgc gtttacggga
     3181 acctccggcg acaccgaggt cgcgttcgct ttgaagtacc tcgacgacta cagcgagagg
     3241 atgtattctt ttgtcaacct gattccgacc gccgaggggg ggacgcacgt cgcaggcttc
     3301 cgggccgccc tgacgagggc ggtgaacgaa gccgccaaga cggagaagct cctgaaggcg
     3361 ggggagggca acatcctcgg cgacgacctt cgcgaagggc tgctctgcgt cctctcggtg
     3421 aaacttccga acccgcagtt cgaggggcag acgaagggaa agctgggcaa cggcgagacg
     3481 accggcgcgg tgagcacgct ggtgtacgac aagctctcct acttcttcgc gaacgagggc
     3541 ggggggatcc tccggatcat cgtcgacaag gcgctctcca cgaggagggc gagggaggcc
     3601 gccaagcggg cgaaggagct ggtcaggagc aaggggggcg gcggaaggga cgcagggctt
     3661 cccggaaagc tcgccgactg tatctcgcgg aagcccgcgg aggcggagct cttcatcgtc
     3721 gagggcgaca gcgccggagg cagcgcgaag caggggcggg acaggaagtt tcaggccatc
     3781 ctccctctgc gagggaagat tctcaacgtc gagaaggcga gcctggataa aatcctcgcg
     3841 aacgccgagg tcggcaatat cgtgaaggcg ctggggtgcg gcgtggggag cgagttcgac
     3901 gtctccaggc tgcgctacgg aaagatcgtc gtcatgaccg acgccgacgt cgacggcgcg
     3961 cacatacgga cgctgctgct gacgctcttc tacaggctgc ttccgcagct cgtctccgag
     4021 gggcgcatct atgcggcgca gccgccgctc taccgggcgg ccaaggggaa ggatgtccgg
     4081 tggctgtaca gcgacgcgga actccgcgag ttcgtcggca ggggaaaa
//
LOCUS       DAOPKU010000246         4118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23856_length_4118_cov_4.287718, whole genome shotgun sequence.
ACCESSION   DAOPKU010000246 DAOPKU010000000
VERSION     DAOPKU010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23856_length_4118_cov_4.287718"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1456
                     /locus_tag="PK849_08470"
     CDS             311..1456
                     /locus_tag="PK849_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPE66197.1"
                     /translation="MGLESRVHSGVHFMQGNYAAVEGAVAAGCDFFAGYPITPANEVS
                     EGMARRLPAVGGAFFQGEDELCSIYAITAASLAGAKAMTATASAGYDYMQEGIEYAVA
                     VEAPIVVLDVMRCRGENFATQADIMQVRWAAAGDHEMIVLAPSTVQEMFDFTVRAFNL
                     AEEYRTPVIVISEMSLALMRERLEIPDASTIPVVNRKRTTKHPSEYLPFKAESEFGVP
                     DFAELGTGYRVIHSINPHDERGDIEWEPHAFERLYKRITGKIRENRKKISTTQRFMLD
                     DAEIALIAFGSEVRPCLDAVHAAREKGIKAGVLKLDAPWPIPEEAIAETAAGANAVVT
                     VEMNIGRYAGEVERIVCGKCRTARATKNLGTPHTPDEILSVIEEVRS"
     gene            1453..2316
                     /locus_tag="PK849_08475"
     CDS             1453..2316
                     /locus_tag="PK849_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE66198.1"
                     /translation="MSAPEMHTNGNPLLKYMRQDKLPHFFCPGCGCGQVVSAFLRACE
                     SLELDLGTMVGVGGVGCTARIPVYINMDCLHGVHGRTLAWATGIKLHKPETKVVVFAG
                     DGDALSIGGNHFIHAARRNLDVTFVVVNNFNFAMTGGQVAPMTPDGAVTMTTPYGSSE
                     PPFDLCKLAVTAGATYVARTLTSSPPQMTTFLKNALSHKGFSVVEILSQCPTHFGRYA
                     LGSGSPKNLVEWIKNHSVPVSAAEKMKPEEIAGKYLIGEFVKTERPVFRGSTVYTAPR
                     QESAERKEAKA"
     gene            2313..2864
                     /locus_tag="PK849_08480"
     CDS             2313..2864
                     /locus_tag="PK849_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE66199.1"
                     /translation="MSRTSIRFCGFGGQGIILSSVILGEAAVTKEGIYAAQSQSYGSE
                     ARGGQCQSEVVLSKTPIGTPTSDQNDIIVAMFQAAYDSHKHEIKKGGAFFVDSGLVPE
                     IGNVDSSVTVWRVPATETAIRLGSKMAGNMVMLGFLQEKTKVVSKESLMETIRGSVKE
                     KFVAMNLAAFEEGVRLAREAAAP"
     gene            2861..3985
                     /locus_tag="PK849_08485"
     CDS             2861..3985
                     /locus_tag="PK849_08485"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66200.1"
                     /translation="MKLYEFQGKALFREAGIPVPKGAVVTASDREGLFAPSAVKAQVL
                     SGGRGKAGGVVLADTPDDAAKAADAILSMELKGEPVKALLVEEKANIVAEYYLAVTFD
                     GESRTPLFMASASGGMDIESVAEHSPEKILKLPVDPLWGLTDYKIRAMADFLGYEDRK
                     EFDGFVRKVWKLFREKDAVLVEINPLVVTDKGLLALDAKVEIDDDARPRRKQLFEAML
                     SEQRSLAGCEAEADHGTITYVPLEGDIGLISDGAGTGMLTLDLIRDMGGEAADFCEMG
                     GLTSPEVMYDAMKEVFSGKPGIKSLLVVLIGGFNRMDEMAEGIVRYLREHPVSIPLVV
                     RLCGTMEEEGKTIMKEAGLPVYDDLRTAVADAVRFAAGGE"
     gene            3987..>4118
                     /locus_tag="PK849_08490"
     CDS             3987..>4118
                     /locus_tag="PK849_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPE66201.1"
                     /translation="MAVLVDGSTRVIVQGITGKSGILQTKSLLDYGTSVVAGVTPGKG
                     "
BASE COUNT          822 a         1203 c         1350 g          743 t
ORIGIN      
        1 cggagatcgc ctcggacatg gatgtgatcg tcatcgcggt ttccaggaga cggcttcttc
       61 ttggagcggg gatgcgctct ccggatctct tcgagcccgt ggagaaggtc atcgggaaaa
      121 aaatgttgca gtatctcgac ctgtagctcc gaaagcgctt gcgctggtcg tcgagccctc
      181 cctgacggga gggttttttt cagcgagcag aagaaaatat atttgacatt ccattttttg
      241 catgataatc tcgtgtgcat gatacattct gcccccgctg tgcgcgggca tacacaatgg
      301 aggtgtatga atgggacttg agagccgagt tcactccggc gtccatttca tgcaggggaa
      361 ctacgctgca gtggaagggg ccgttgccgc cgggtgcgat tttttcgcag gctatcccat
      421 tacccctgcg aacgaagtct ccgagggaat ggcaagacga cttcccgccg tgggaggcgc
      481 ttttttccag ggtgaggacg aactgtgttc aatttacgca atcaccgctg cgtcccttgc
      541 cggagccaag gcgatgacgg cgaccgcgag cgcggggtac gactatatgc aggaggggat
      601 cgagtacgcc gtcgcggtgg aggcgcccat agtggtgctc gacgtgatgc gctgccgggg
      661 cgagaacttc gccacgcagg ccgacatcat gcaggtgcgg tgggctgcgg ccggggatca
      721 cgagatgatc gtgctcgcgc cgtcgaccgt ccaggagatg ttcgacttca cggtccgtgc
      781 gttcaacctc gccgaggagt accggacgcc ggtcattgta atctcggaga tgtcgctggc
      841 gctgatgcgc gaacggcttg agattccgga tgcttcgact atcccggtcg tgaaccgcaa
      901 acggacgacg aaacacccct ccgagtatct gcccttcaag gcggagagcg agttcggcgt
      961 gcccgacttc gccgagctgg ggacggggta ccgggtcatt cactcgatca acccgcacga
     1021 cgagcgcggg gatatcgaat gggagccgca cgccttcgaa cgcctctata aaagaattac
     1081 cgggaagatc cgggagaaca gaaagaagat ctcaaccacg cagcgtttca tgctcgacga
     1141 cgcggagatt gcgctcatcg ccttcggcag cgaggttcgc ccctgtctcg acgctgttca
     1201 tgctgcgcgc gagaaaggga tcaaggccgg agttctgaag ctcgatgcgc cgtggccgat
     1261 ccccgaggaa gcgatcgccg agacggcggc tggcgcaaac gccgtcgtga cggtcgaaat
     1321 gaacatcggc aggtacgccg gagaggtgga gcgcatcgtg tgcggaaagt gccgcaccgc
     1381 gcgggcgacc aagaacctcg gaacgccgca cactccggat gaaattctct ccgtcatcga
     1441 ggaggtgcgg tcatgagcgc tcccgaaatg catacaaacg gcaatccgct gctcaagtac
     1501 atgcggcagg acaaactgcc ccatttcttc tgccccggct gcggctgcgg tcaggtggtc
     1561 agcgcctttc tccgtgcgtg cgagtcgctc gaactggacc tcgggacgat ggtcggcgtc
     1621 ggcggcgtcg gctgcacggc gcgcattccg gtatacatca acatggactg cctccacggc
     1681 gtccacggca gaacgcttgc ctgggcgacc ggcatcaagc tgcacaagcc ggagacaaaa
     1741 gtcgtggtct tcgccgggga cggcgacgcg ctctccatcg gcggcaacca cttcatccac
     1801 gcggcgcgcc ggaatctcga cgtcaccttc gtcgtggtga acaacttcaa cttcgcgatg
     1861 acgggcggac aggtcgcccc catgacgccc gacggcgccg tgaccatgac caccccctac
     1921 ggaagcagcg agccgccttt cgatctctgc aagctggccg tcaccgccgg agcgacgtat
     1981 gtcgcccgta cgctgacctc cagtccgccg cagatgacga cgttcctgaa gaacgcgctc
     2041 tcccacaagg gattctccgt cgtggagatt ctctcccagt gtccgaccca cttcggcagg
     2101 tacgcgctcg gcagcggaag cccgaagaac ctcgtcgagt ggatcaagaa tcacagcgta
     2161 cccgtatccg cagcggaaaa gatgaagccc gaagagatcg ccggaaagta tctcatcggc
     2221 gagttcgtga agacggagcg tcccgttttc cgggggagta cggtctatac ggctccgcgc
     2281 caggagagtg cggagcgcaa ggaggcgaaa gcatgagccg gacgagcatc cgattctgcg
     2341 gtttcggcgg ccagggaatc atcctctcct cggtgattct tggagaagcc gcggtcacga
     2401 aggaagggat ctacgcggcc cagtcccagt cgtacggctc cgaggccaga ggggggcagt
     2461 gccagtcgga ggtcgtgctc tcgaagacgc ctatcggcac gccgacgagc gatcagaacg
     2521 acatcatcgt cgccatgttc caggcggcgt acgacagcca caagcacgag attaaaaagg
     2581 gcggggcctt tttcgtcgac agcggccttg tgccggagat tggcaacgtc gattcctccg
     2641 tcacggtctg gagggtaccc gcgactgaaa ctgcgatccg tctcgggagc aagatggcgg
     2701 gaaatatggt catgctcggc ttcctccagg agaagacgaa ggttgtttcg aaggagagcc
     2761 tgatggagac aatccgcggg tcggtgaagg agaagttcgt cgccatgaac cttgcggcct
     2821 ttgaagaggg cgtcaggctc gcgcgggagg cggctgcccc atgaaactct acgaattcca
     2881 ggggaaggcg ctcttccggg aggccggtat ccccgtgccc aaaggagcgg tcgtcacagc
     2941 ctccgaccgg gaagggcttt tcgccccgtc ggccgtgaag gcgcaggtgc tctccggggg
     3001 aagagggaag gcggggggcg tcgtccttgc ggacacgccc gacgacgcgg cgaaggcggc
     3061 cgacgccatt ctctccatgg agctgaaggg agagccggtg aaggcgctcc tcgtcgagga
     3121 gaaggcgaat atcgtagcgg agtactacct tgccgtcacc ttcgacggag agtcgaggac
     3181 cccgctcttc atggcgagcg cttcgggcgg gatggacatc gagtccgtcg cggagcattc
     3241 gcccgagaag attctgaagc ttcccgtcga tcctctctgg gggctgaccg actacaaaat
     3301 tcgcgcgatg gcggacttcc tcggatacga agaccgcaag gagttcgacg gattcgtccg
     3361 gaaggtctgg aagctcttcc gcgagaagga cgccgtcctt gtggagatca atccgctcgt
     3421 cgtcaccgat aagggactcc ttgcgctcga cgcgaaggtg gagatcgacg acgacgccag
     3481 gccccgccgg aagcagctct tcgaggcgat gctctccgag cagcggtctc ttgctggctg
     3541 cgaagcggag gccgaccacg gcaccatcac gtacgtcccg cttgaagggg atatcggcct
     3601 catctccgac ggcgccggga cgggcatgct cacgctcgac ctgatccgcg acatgggagg
     3661 cgaggcggca gacttctgcg agatgggcgg cctgaccagc cccgaagtca tgtacgacgc
     3721 catgaaggag gtcttctccg gcaagcccgg tatcaagagc ctgctcgtgg tgctcatcgg
     3781 cggtttcaac cgcatggacg agatggcgga gggcatcgtg cgctatctca gagagcatcc
     3841 tgtctccatc cctcttgtgg ttcgtctctg cggcaccatg gaggaagagg ggaagaccat
     3901 catgaaagag gcgggacttc ccgtctacga cgatcttcga accgccgtgg cggatgcggt
     3961 gcggttcgcc gcgggaggcg agtgaaatgg ccgttctggt tgatggaagc acgcgggtca
     4021 tcgttcaggg gatcacggga aagtccggaa ttcttcagac gaagtcgctt cttgactacg
     4081 gaacatccgt ggtcgccggg gtgacgccgg gcaagggg
//
LOCUS       DAOPKU010000247         4109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23933_length_4109_cov_4.357918, whole genome shotgun sequence.
ACCESSION   DAOPKU010000247 DAOPKU010000000
VERSION     DAOPKU010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23933_length_4109_cov_4.357918"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PK849_08495"
     CDS             <1..763
                     /locus_tag="PK849_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPE66202.1"
                     /translation="LMCLIVLFLLSLYGTIIAGCFVKNWGIDYTFTLENIREAFERGW
                     SVLLDTLTLAGAATPIAGLLAMITALVLARKKFPGKRLLDMLILTPFALPGTLVGISY
                     ILAFNKAPLILVGTGAIIVISYVIRELPVGVEAGIASLKQIDPAIEEAAADLGADTPT
                     VFRTIVLPLIRPAFISSLSYTFVRSMTAVSAVIFLISARWYHLTVLIYNFSENLRFGL
                     ASVLASTLIVIVLLTFGLLRLLVRKNENLAKTMSL"
     gene            776..1873
                     /locus_tag="PK849_08500"
     CDS             776..1873
                     /locus_tag="PK849_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66203.1"
                     /translation="MDIQSKSVTLEQVSKIFKDPQSGKDVHAVDGADFVIAPGELVTL
                     LGPSGCGKTTTLRMIAGFELPTKGKIRIGAEDITFLPPNKRDTAMVFQSYGLFPHMNV
                     FDNVAYGLRLRKIPEDAISEKVMRFLGMVGLDTLAKRPPSRLSGGQQQRVALARSLIV
                     EPAVLLLDEPLSNLDALLREQMRVEIRRIQKSLGITAAYVTHDRVEAMSLSDRIIVMN
                     KGKIVQIGTPHQVYRDPVDAFVAGFIGKVAFFPAEVRGIDEVCTVDVRGKTFRLPRFS
                     EELKGAQRALVMCRPESLTLLPPGEGILDGRVTTNVYLGHSIESFVATEMGEILVQVD
                     NPDARRVFGEGEAVSVSFIPELTKALPAGEQ"
     gene            complement(1959..3353)
                     /locus_tag="PK849_08505"
     CDS             complement(1959..3353)
                     /locus_tag="PK849_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HPE66204.1"
                     /translation="MKKRKGLTWYLFLFAVLLLPLSGCSLTNSTENSNEARALAEEAY
                     IFAYPMLENYRTMQVQAISPDRFNRFKHATEIMGPEYKDIVRPNNDTLYSTLWLDLRA
                     EPMVLHIPPVTGRYYSFQFVDMYTHNFAYTGVRTTGTRGGVFMVAGPEWNGEKPAGVN
                     EVFRSEGNFVLCLVRTALEGPEDLENVHAIQSRYDVEPLSGFLGVPPPAPSPMDVFPL
                     YLQPAAESVHFINYFNFLLGRLKVHPSEEELIERFGAIGIGPGFYFNADNFSPEIRAA
                     IEAGIASAIKQIEASREILGERKNGWVLTRRIFGNRERMQGLYLVRAGAARMGLYGND
                     LEEAYYPTGNLDADGELFDASKHSYVLRFEKDEIPPVDAFWSITMYNLPEQLMVENPI
                     GRYSIGDRTEGLTFGEDGSLELYFSKTSPGGEKERNWLPAPDGPFSLTLRMYLPHEHA
                     LDPLYAPPVIRKAE"
     gene            complement(3659..>4109)
                     /locus_tag="PK849_08510"
     CDS             complement(3659..>4109)
                     /locus_tag="PK849_08510"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HPE66205.1"
                     /translation="QGLYLVRAGAAKAMLYGDNAEEVSYLTANLDEDGDVLDASMRSY
                     ALTFAKEELPPVDAFWSITMYSLPEQALTPNPLNRYSLGSKSPSLVYGADGSLTLYLF
                     KESPGESREGNWLPAPDGPFSLTLRMYLPKQDAIPPHYVPPPLRKVR"
BASE COUNT          844 a         1290 c         1165 g          810 t
ORIGIN      
        1 gctgatgtgc ctcatcgtcc tcttccttct gagcctctac ggcaccatca tcgccgggtg
       61 tttcgtgaag aactggggca tcgactacac cttcaccctg gagaacatcc gggaggcgtt
      121 cgagcgcgga tggagcgtcc tgctggacac gctaaccctc gccggggctg ccacgcccat
      181 cgcaggcctg ctcgccatga tcacggccct cgtgcttgcc agaaagaagt tccccggcaa
      241 gcggctgctc gatatgctca tcctgacgcc cttcgccctt cccggaacgc tggtcggtat
      301 cagctacatc ctcgccttca acaaggcccc gctcatcctc gtcgggacgg gcgccatcat
      361 cgtcatcagc tacgtcatcc gagagctgcc ggtgggggtc gaagcgggca tcgcctcgct
      421 gaaacagatc gacccggcga tcgaggaggc cgctgcggac ctcggggcgg acacgcccac
      481 tgtcttccgc accatcgtcc tcccgctcat caggcccgcc ttcatctcga gtctgtcgta
      541 caccttcgtc cgctccatga cggcggtcag cgcggtcatc ttccttatct ccgccaggtg
      601 gtaccacctc accgtgctga tctacaactt ctccgagaac ctgcgcttcg gccttgccag
      661 cgttttggcg agcacgctca tcgtcatcgt gctcctgacg ttcggcctcc tccgtcttct
      721 ggttcggaag aacgaaaacc tcgcgaagac catgagtctc taaggtggga tagttatgga
      781 catccaatcg aaatcagtca cactcgaaca ggtaagcaag atattcaagg acccgcagtc
      841 gggaaaggac gtgcatgccg tcgacggcgc cgacttcgtc atcgcaccgg gagagctggt
      901 cacgctgctc ggtccgtccg gctgcggcaa gacgacgacg ctccgcatga tcgcggggtt
      961 cgaactgccg acgaagggga agatccgcat cggcgccgaa gacatcacct tcctccctcc
     1021 caacaaacgg gatacggcga tggtcttcca gagctacgga ctcttcccgc acatgaacgt
     1081 cttcgacaac gtggcctacg gtctgcgcct gcgaaaaatc ccagaggacg caatctcgga
     1141 gaaggtcatg cgcttcctcg gcatggtggg gctcgacacg ctcgccaagc gccccccgtc
     1201 ccgtctctcc ggaggccagc agcagcgcgt ggcccttgcg cgctccctca tcgtggagcc
     1261 cgccgtcctg ctgctcgacg aaccgctctc caatctcgac gcgctgctcc gcgagcagat
     1321 gcgcgtggag atccgccgta tccagaagtc gctcgggatc accgccgcat acgtcaccca
     1381 cgaccgggtg gaggcgatga gcctctccga caggatcatc gtcatgaaca aggggaagat
     1441 cgtgcagata ggcacgccgc accaggtcta ccgcgacccg gtggacgcct tcgtcgcggg
     1501 tttcatcggc aaggtggcct tcttccccgc ggaggtacgg ggcatcgacg aagtctgcac
     1561 ggtcgacgtc aggggaaaga ccttccgtct tccgcgcttc tcggaagagc tgaaaggcgc
     1621 gcagcgcgcc cttgtcatgt gccgccccga gtcgctcact ctgcttcctc cgggagaggg
     1681 catcctcgac ggccgcgtca ccaccaacgt ctaccttggg cacagcatcg agtcgttcgt
     1741 cgccacggag atgggagaga tcctcgtaca ggtggacaat cccgatgcac ggcgcgtctt
     1801 cggagaggga gaagccgtct ccgtctcttt catcccggaa ctgacaaaag cgctgcctgc
     1861 cggggagcag taatcgttca aagatataca aagcggagcc ccggaaaaac cccgggactc
     1921 cgctttttgt gtgcacatcg tcaaaaaccg ggctttcgtc actccgcttt tcggatcacc
     1981 ggcggggcat acagcgggtc gagagcgtgc tcatggggca ggtacatccg caacgtcagc
     2041 gagaaggggc cgtccggcgc gggaagccag ttccgttcct tctccccgcc cggcgaagtc
     2101 ttgctgaagt agagctccag ggagccatcc tcaccgaacg tcagcccctc cgtacggtcg
     2161 ccgatggagt accggccgat cgggttttca accatcagct gctccggaag gttgtacatg
     2221 gtgatggacc agaacgcatc gactggcggt atctcatcct tctcgaaccg gagaacatac
     2281 gagtgcttcg aagcgtcgaa cagctcgccg tccgcatcga gattgccggt aggatagtat
     2341 gcctcttcga ggtcgttccc gtacaacccc atacgcgcag ctccggcgcg gacgagatag
     2401 agcccctgca tccgctccct gttcccgaaa atacgtctgg taagcaccca tccgttcttt
     2461 ctctctccga gaatctcccg ggaggcctcg atctgcttga tcgcggacgc aatccccgct
     2521 tcgatcgcgg cgcgaatctc cggactgaaa ttatcggcgt taaagtagaa accggggccg
     2581 atgccaatgg cgccgaaacg ctcgatcagt tcctcttccg aaggatggac cttcagccgg
     2641 ccaagaagaa agttgaagta gttgatgaaa tggacggatt cagcggcagg ctgcagatag
     2701 agcggaaaga cgtccatcgg ggaaggcgcg ggcggaggga ctcccagaaa accgctcagc
     2761 ggctcgacat cgtaccggct ctgaatggcg tgcacattct ccaggtcctc cggtccttca
     2821 agcgcagtcc gtaccaggca gaggacgaaa ttgccctccg accggaagac ctcgttcact
     2881 ccggcaggtt tttcaccgtt ccattccgga cccgcgacca tgaacacgcc gcctcttgtc
     2941 ccggtcgtcc ggacgccggt gtaggcgaag ttatgcgtat acatgtccac aaactggaat
     3001 gaatagtagc ggccggttac cggaggaata tgcagcacca tcggctccgc cctcagatcc
     3061 agccagagcg tggagtacag cgtatcgttg ttcggacgga caatatcctt atattcgggc
     3121 cccatgatct ccgttgcgtg cttgaaacgg ttgaaacgat ccgggctgat cgcctgaacc
     3181 tgcatcgtcc tgtagttctc aagcatcggg tacgcgaaga tatacgcttc ctcggcaagc
     3241 gcgcgggctt cgttggagtt ctccgtgctg ttcgtcaggg aacatcctga gagcggcaga
     3301 aggagcaccg caaaaagaaa cagataccac gtcagtcctt tccgtttttt catagtaatc
     3361 ttcctttctt gttgctctcc cccaggatag aggtctgctg aggtcagtaa aaatacgtag
     3421 ctccattgta tcagaaaaac agattttcag gcataaattc ggccccaaaa acatgcgacc
     3481 aaaaccctcc aactgaagag tcgactccgg aggtcggttg tttatgcaaa gcgatcccca
     3541 agcaaccgat gaagcgattc ctcggaaccc ttcgctcgca tttttgcccg gaatgagatc
     3601 cggcattgaa aaagagagag cctcctgatc agggaggctc tctccggata tcgctttttc
     3661 agcgcacttt tcgtaaagga ggcggcacat agtgcggcgg gatcgcgtcc tgtttcggca
     3721 ggtacatccg aagcgtcagc gagaagggac cgtcgggagc gggaagccag ttcccctccc
     3781 tcgactcgcc gggggattcc ttgaagaggt acaacgtcag cgagccgtcg gcgccataga
     3841 caagcgaagg actcttgctt ccgagagaat acctgttcag tggattaggt gtcagcgcct
     3901 gctcaggcag cgagtacatg gtgatcgacc agaacgcgtc gacggggggc agctcctcct
     3961 ttgcgaaggt aagagcgtac gagcgcattg aggcgtccag aacatccccg tcctcatcga
     4021 gattcgcggt cagataagag acctcttcgg cgttatcgcc gtagagcatc gccttggcgg
     4081 ctcccgcccg gacgagatag agcccttgc
//
LOCUS       DAOPKU010000248         4103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_23976_length_4103_cov_5.153162, whole genome shotgun sequence.
ACCESSION   DAOPKU010000248 DAOPKU010000000
VERSION     DAOPKU010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23976_length_4103_cov_5.153162"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PK849_08515"
     CDS             <1..655
                     /locus_tag="PK849_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HPE66206.1"
                     /translation="PFLLASFVFGVFIYTIAKVSGASNGLAFFLGLLFGGAGTGYMLF
                     FFRDPEIVTPAEPGIVVAAANGKLAKIVEMYEKDYLKQDTIRISIFLSLFDVHVNRFP
                     IGGFSTFLGYFPGKRLFTFDEKSSDVNQHNAILVRNEETSCLIRQIVGPVCRRVVYWL
                     REDKTTTVKQGDRFGMMKFGSRLDMYFPKDDIEMVAKIGDQVTAGETIIARIVKKGK"
     gene            659..1456
                     /locus_tag="PK849_08520"
     CDS             659..1456
                     /locus_tag="PK849_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPE66207.1"
                     /translation="MTGQQVHKLRILANWRRIVPTMVTLSALFFGFASILSTIEAMRL
                     DVPALLGRSAQYIMLALILDGLDGNVARWLKGTTEFGAELDTFVDFLAFGVAPAALLY
                     VMLIHGPSPFWRTILPSAIVFSGALRLSRFRLKDPLRGQGGFIGLPITVNSSWIALAA
                     FAAQIMYPEGHILVTGPVSIVFQFLILVMISLQLSNLRYPKPSNKVRFFAPAVLAVLL
                     LWALSETYAAWIATVLVFGCFVYVLTGPMIHKRSALRQQQQQHSEGK"
     gene            1461..2327
                     /locus_tag="PK849_08525"
     CDS             1461..2327
                     /locus_tag="PK849_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPE66208.1"
                     /translation="MRAIGVDLGGHKIAAALVEDGRIVERLSEPTVGREPDGILSQIA
                     AMVQRLGVCAECPVGVGIPGVLDAPREVALLLPNFTGWNGLPIRKMLSSKIGLPVELE
                     NDANCYALGEGWGGAARGMTDYILLTLGTGIGGGIVIGGKILKGFHGMAGEPGHLVVG
                     SGEPCGCSSHGHLEGISGADALERTAKSMGLQPDLKYLWTRRTDPAVAPLWDRGLDHL
                     AKGIASAIHLFDPQAVILGGGMSRGEGFIDVLKPRVVDYLALPFRKTLDLRPSLLGND
                     APTIGAAALTRK"
     gene            2341..3354
                     /locus_tag="PK849_08530"
     CDS             2341..3354
                     /locus_tag="PK849_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66209.1"
                     /translation="MRTDTNAHTPPAATLLSPEAFLYSASIPGFFSALPLDERSSLSV
                     ATGRSALAKALDAASQKALTGRAWLPSLCCASLAPVFLRRGFSISFYSTAPFILPRLE
                     RGDVFLYIHFCGFPNRIAERALESLPEERRPIVVEDCVHALFTRGTGRFGDFVLYSFR
                     KFLPVPDGGLLLSREPFDDTLSPPLERFISLKTAGLLSESRRLLEEGEEALDADDEAR
                     EPSVMGALLLDRLPWTHIPEARRRNYAFLAERLCLQPLDDGTVPLGLPFRISAGKAEL
                     ERRLRAAGFEPPLSWDAPRDAPSDEADRLVVLPCDERMEERELARLVRICKTFSAERL
                     AAQ"
     gene            3393..3794
                     /locus_tag="PK849_08535"
     CDS             3393..3794
                     /locus_tag="PK849_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPE66210.1"
                     /translation="MPEICSQITFFYYKNFERARDFYERIMGFELADDQGTCRIYRVQ
                     GTAFLGIVDENHGRCSAPRSESNVLVTLATNDVKGWWEHLRKEGVKITSELLWKPEIS
                     IEAFFFEDPEGYALEVQSFLKPELRKIFGQE"
     gene            complement(3863..>4103)
                     /locus_tag="PK849_08540"
     CDS             complement(3863..>4103)
                     /locus_tag="PK849_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPE66211.1"
                     /translation="IIRIVGLILVIALLTIPPSVAEKFSRSLWSMMGISCVLSALFSV
                     AGLALAVQFNLTAGAVIIAVASAVYLVSLLLPGRR"
BASE COUNT          755 a         1334 c         1222 g          792 t
ORIGIN      
        1 accgtttctt ctggcgtcgt tcgtgtttgg cgtcttcatc tacacgatag ccaaggtatc
       61 gggcgcttcc aacggactcg ctttcttcct cgggctgctc ttcggcggcg cgggaacggg
      121 gtacatgctt ttcttcttcc gcgatccgga gatagtcaca ccggcggaac cgggcatcgt
      181 ggtcgccgcc gcgaacggca agctggcgaa gatcgttgaa atgtacgaga aggactatct
      241 gaaacaggac acgatccgca tcagcatctt cctcagcctg ttcgacgtcc atgtgaaccg
      301 cttcccgata gggggcttct cgaccttcct tggctacttc cctgggaaac ggctcttcac
      361 cttcgacgag aagtcgtcgg acgtgaacca gcacaacgcc attctggtac gcaacgaaga
      421 aacctcgtgt ctcatacggc aaatcgtcgg ccccgtctgc cgccgcgtcg tctactggct
      481 cagggaggac aagacgacca ccgtaaagca gggcgaccgt ttcggcatga tgaagttcgg
      541 ctcgcgcctt gatatgtact tccccaagga cgacatcgag atggtcgcga agatcggcga
      601 ccaggtcacc gcgggcgaga cgatcatcgc ccggatagtg aagaaaggaa agtaaacaat
      661 gacaggacag caagtgcata aattgagaat tctcgccaac tggagacgga tcgtcccgac
      721 catggtgacg ctctccgcgc tcttcttcgg cttcgccagc atactctcca ccatcgaggc
      781 gatgcgcctc gatgttcccg cgctgcttgg gcgttccgcg cagtacatca tgctcgcgct
      841 cattctcgac gggctcgacg gcaacgtcgc ccgctggctg aaagggacga ccgagttcgg
      901 cgcggagctg gataccttcg tcgacttcct cgccttcggc gtggctccgg cagcgctgct
      961 ctacgtcatg ctcattcacg gccccagccc gttctggcgc accattcttc cctctgccat
     1021 cgtcttctcc ggcgcgctcc gcctttcgcg cttccggctg aaggatcctc tgcgcggcca
     1081 gggcggcttc atcggccttc ccatcacggt gaactcttcg tggatcgcgc tcgcggcctt
     1141 cgccgcgcag attatgtacc ccgaggggca catccttgtc accggcccgg tctccatcgt
     1201 cttccagttc ctcatcctgg tgatgatctc gctgcagctc tccaacctgc gctaccccaa
     1261 gccgtccaat aaggtgcgct tcttcgcccc ggcggttctc gccgttcttc ttctgtgggc
     1321 gctcagcgag acgtatgccg cgtggattgc caccgttctg gtctttggat gcttcgtcta
     1381 cgtgctcaca ggcccgatga tccacaagcg ttcggctctg cgtcagcagc aacagcagca
     1441 tagcgaagga aaataagatc atgcgggcca tcggcgtcga tctgggagga cacaaaatag
     1501 ccgcagctct cgtcgaagac ggacggatcg ttgaacgtct ctccgaaccg acggtcgggc
     1561 gggagccgga cggtattctc agtcagattg cagccatggt ccaacgcctt ggcgtgtgcg
     1621 cagaatgccc ggtgggagtg ggaatccctg gcgttctcga cgccccgcgc gaggttgcgc
     1681 tgctcctccc gaacttcacc ggatggaacg gcttgccgat caggaagatg ctctcatcga
     1741 agatagggct gccggtggaa ctcgaaaacg acgccaactg ctacgcgctc ggcgaaggct
     1801 ggggcggcgc ggcccggggg atgaccgact acatcctcct cacgctcggc accggcatcg
     1861 gcggcggcat tgttatcggc ggcaaaatac tcaaaggctt ccacggcatg gcgggcgaac
     1921 cgggacatct cgtcgtcggc tccggagagc cgtgcggctg ctcctcccac gggcacctcg
     1981 aaggcatcag cggcgcggac gccctggagc gcacagcgaa gtcgatgggt ttgcagcccg
     2041 acctgaagta cctctggacc cgcaggaccg acccggctgt agcgcctctg tgggaccgag
     2101 ggctggacca cttggcgaag gggatcgcct cggcgattca cctcttcgac ccgcaggcgg
     2161 tcatcctcgg cggcggcatg agccgcggcg aggggtttat cgacgtcctg aagccaagag
     2221 tagtggacta ccttgcgctc cctttccgca agacgcttga tctgcgcccg agcctcctcg
     2281 gcaacgacgc gcccaccatc ggcgccgcgg ccctcactcg gaagtaagcg cctcgcagac
     2341 atgcgcaccg atacaaacgc tcatacgcct ccggcggcca cgttgctgtc tccggaggct
     2401 tttttgtact ccgcctccat ccccggtttt ttctccgccc tcccgctcga cgaacgctcc
     2461 tcgctctccg tcgccacagg aagatcagca cttgcaaagg cgctcgacgc cgcttcccaa
     2521 aaagcgctta cggggagagc gtggctcccc tcgctctgct gcgcgtcgct cgcccccgtt
     2581 tttttgcgga gggggttctc catctccttc tacagtacgg cgccgtttat cctccccaga
     2641 cttgaacgag gcgacgtctt cctgtacatc cacttctgcg gcttcccgaa caggattgcc
     2701 gagcgggcgt tggaatccct gccggaggag cgccgcccga tcgtcgtcga ggactgcgtc
     2761 cacgccctct tcacacgagg aacaggacgc ttcggcgact tcgtgctcta cagcttccgc
     2821 aagtttctcc ccgtcccgga cggtgggctg ctcctctccc gcgagccgtt cgacgacacg
     2881 ctctctccgc cgctcgaacg cttcatctcc ctcaagacgg cggggcttct gtcggaatcg
     2941 cgccgccttc tcgaagaggg agaggaggcg ctcgacgcgg acgacgaggc cagagagcct
     3001 tctgttatgg gagcgctcct tctcgaccgg ctcccgtgga cgcacattcc cgaagctcgc
     3061 agacggaact acgccttcct cgcggaacgc ctttgcctgc agccgctcga cgacggcacg
     3121 gttccgctcg ggcttccgtt ccgcatatcc gcgggaaaag ccgaactcga acgccgcctt
     3181 cgggccgccg gtttcgagcc gcccctctca tgggacgctc cccgcgacgc tccctcggac
     3241 gaggcagaca ggctcgtcgt tttgccgtgc gacgagcgga tggaggagcg ggagcttgcg
     3301 cggctcgtgc gcatctgtaa aacgttctca gcggagcgcc ttgctgcaca ataacggcgc
     3361 agcaaggccg aaatcaatgg gagggatgct tcatgcctga aatctgttca cagatcactt
     3421 ttttttacta caagaacttc gagagagcgc gcgactttta cgagcggatc atggggttcg
     3481 aactcgcgga cgaccaggga acgtgccgaa tctaccgcgt tcagggaacg gccttcctcg
     3541 gcatcgtcga cgagaaccac gggcgctgct ccgcgccccg ctcggagagc aacgtcctcg
     3601 tcacgctggc gacgaacgac gtcaaggggt ggtgggagca cttgcggaag gaaggcgtca
     3661 agataacctc cgagctgctt tggaaaccgg agatttctat cgaggcgttc ttcttcgagg
     3721 atccggaggg gtacgccctc gaagtccagt ctttcctgaa accggagctg cggaagattt
     3781 tcgggcagga gtgatacggc gctacgcggc gaaacgtgaa gcggtagctc ccctccgaag
     3841 tacaaagagc ggaggagggg agttaccgtc tccccggcag tagcagcgac accagataga
     3901 ccgccgaggc caccgcgatg atcaccgcgc ccgcggtgag gttgaattgt accgcgaggg
     3961 cgagcccggc gacggagaag agcgcgctca aaacgcacga aatgcccatc atcgaccaaa
     4021 gcgagcggga gaacttctcc gccacggatg gggggatggt gagcagcgcg atgaccagaa
     4081 tcagcccgac gatgcggatg atg
//
LOCUS       DAOPKU010000249         4090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24082_length_4090_cov_3.794548, whole genome shotgun sequence.
ACCESSION   DAOPKU010000249 DAOPKU010000000
VERSION     DAOPKU010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24082_length_4090_cov_3.794548"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2405)
                     /locus_tag="PK849_08545"
     CDS             complement(<1..2405)
                     /locus_tag="PK849_08545"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPE66212.1"
                     /translation="MTESEARTRKERIDARLRSSVLGWTLIRSDEVRDCSSLTRHAVE
                     EYPTETGPADYALFADGKLLGIIEAKKVSLGAENVLEQAKRYARGVPDTVGEWRGYRV
                     PFLYSTNGESIFHLDVRRKENTSRLLADFHSPRALLEKYRKDTESAERWFAQRPVAEI
                     TRLRPYQIAAVEAIEKALCAGKKSMLIAMATGTGKTFTLVSSIYRLLKSGYARRILFL
                     VDRRALAAQTVSALSAFQTPEGLKLDRDYEVYSQRFRREELEDGSSFDPKVLPEEYLT
                     NPREAHTFIYVSTIQRMAINLLGKDADGGFEYDTEADRLDIPINAFDVIIADECHRGY
                     SAKETGCWKYVLDYFDAVKIGLTATPAAQTLGMFKHKVYSYSTEQAVLDGYLVDYDAV
                     KITSDIHINGAFLKEGEMVGEIDVETGSEQLDSLEDEREFSATEIEQKITAPDSTRKI
                     LTAIKKYTDQHEAEHKRFPKMLIFAVNDLPHVSHADEVVRTCKEVFGKGDDFVMKITG
                     SPTVDRPLQKIRQFRNRPEPKIVVTVDMLTTGVDIPAIEMIVFMRMVKSRILWVQMLG
                     RGTRLCREINKEKFTIFDCFDGSLIEYFRNATDFDVTLQKETVSLAEIIERIYDNRDR
                     EYNVNRLIKRLRRVQNTMGAEAREAFARYIPDGDMKAYADGLKENIQNDFPGTMELLR
                     NKEFQDLLENYPRPKKVFFKGYDIVDTVDDEVMFRVGDDYQKPQDYLRLFEEFVRKNP
                     EQIEAIEVLLSRPSGWNADVLDDLRDKLRKSHFSEKDLQRGHELVYKKPLADIISMVK
                     HAS"
     gene            complement(2463..3608)
                     /locus_tag="PK849_08550"
     CDS             complement(2463..3608)
                     /locus_tag="PK849_08550"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HPE66213.1"
                     /translation="MNTVPASIPVLRWAAERAHLSDTALERSFRKWDLWLSGDALPTL
                     KQLEDFARLTHTPIGYFFLPEPPALQLPIPDFRTRRDEALAEPSAALLDTLYLCQQRQ
                     DWYREYARIHGFARLEFVSSASEDVQPEQVADSMRQVLGLSVADRRRLSTWTDALRQL
                     ISRAEDTGILVMASSIVGSNSHRKLDATEFRGFALVDDRAPLIFINASDSKAAQMFTI
                     AHELAHIWSGESGVSDPEAGSVPKQGLERWCNRVAAELLMPLDALNEAYDIDAPVADE
                     IQRLAHLFKVSSLVALRRLFDAGFIDKDTLWLNYRQEVERIRALDRGTGGGGDFYRTL
                     GARTGKRFARAVFSSTLEGQTLFRDAFRMLGMRKTSTFYEAARVEGF"
     gene            complement(3882..>4090)
                     /locus_tag="PK849_08555"
     CDS             complement(3882..>4090)
                     /locus_tag="PK849_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66214.1"
                     /translation="APSGIRHVEIANWSGQAIACPRSRLNKLRDWVEAQRPGVYFLLE
                     KQTAETGDRNSDRFPPEFMFRLSN"
BASE COUNT          762 a         1112 c         1242 g          974 t
ORIGIN      
        1 tccgaagcgt gcttgaccat ggagatgatg tccgcaaggg gctttttgta gacaagctcg
       61 tggccccgct gcaggtcttt ctcggagaaa tggctttttc tgagcttgtc gcgcaggtcg
      121 tccagcacat cggcattcca gcccgaaggg cgggagagca atacttcgat ggcttcgatc
      181 tgctcggggt tctttcgaac gaactcttcg aagagccgaa ggtagtcctg gggtttctgg
      241 tagtcgtctc cgacccggaa catcacttcg tcgtccaccg tatccacgat gtcgtacccc
      301 ttgaagaaga cctttttcgg tcgggggtag ttttcgagca ggtcctggaa ctccttattc
      361 cgaagaagct ccatggtccc gggaaagtcg ttctggatat tctctttcag cccgtcggcg
      421 taggctttca tatcgccgtc gggtatgtag cgggcgaagg cttcccgggc ttcggcgccc
      481 atggtgttct ggacgcgccg cagccgtttg atcagacggt tgacattata ctcccggtcg
      541 cggttgtcgt agattcgttc gatgatttcg gcaagggaga ccgtctcttt ctggagggtg
      601 acgtcgaaat cggtggcatt ccggaagtat tcgatgaggc ttccgtcgaa gcagtcgaag
      661 atggtgaact tctctttgtt gatttccctg cacagccggg ttccgcgccc aagcatctgc
      721 acccagagga tacgggactt caccatccgc atgaaaacga tcatttctat ggcggggata
      781 tccacgccgg tggtcagcat gtccacagtg acgactattt tgggctcggg gcggttgcgg
      841 aactggcgta ttttctgcag ggggcggtcg acggtggggc ttccggtgat tttcatcacg
      901 aagtcgtcgc ccttgccgaa cacttccttg caggtgcgca ccacttcgtc ggcgtgggag
      961 acatggggaa gatcgttgac ggcgaagatc agcattttgg ggaaccgctt gtgctctgcc
     1021 tcgtgctggt cggtgtattt tttgatggcg gtgagaattt tcctggtgct gtcgggagcg
     1081 gtgatcttct gctcgatttc cgtcgcgctg aactcccgtt cgtcctccag cgagtcgagc
     1141 tgctccgagc ccgtttccac gtcgatttcg ccgaccatct ccccttcctt gaggaacgcg
     1201 ccgttgatat ggatgtcgga cgtgatcttt acggcgtcgt agtccacgag gtagccgtcg
     1261 aggacggcct gttcggtgct gtaggaatag actttgtgtt tgaacatgcc cagggtttgg
     1321 gccgccggtg tggcggtgag gccgattttc acggcgtcga agtagtccag gacatatttc
     1381 cagcagccgg tttcttttgc ggagtatccc cggtggcact cgtcggcgat gatgacgtcg
     1441 aaggcgttga tggggatgtc gagcctgtcg gcttcggtgt cgtactcgaa gccgccgtcg
     1501 gcgtctttgc ccagcaggtt gatggccatt cgctggatgg tggagacgta gatgaaggtg
     1561 tgggcttccc gggggttggt aaggtactct tcgggcagca ccttggggtc gaagctgctg
     1621 ccgtcttcca gctcttcgcg ccggaatctc tggctgtaga cctcgtaatc cctgtccagt
     1681 ttgagcccct ccggcgtttg aaaggcggag agggcggaaa cggtctgggc ggcgagggcg
     1741 cgccggtcca ccaggaagag aatccgccgg gcgtagccgg atttcagcag gcggtagatg
     1801 gaggaaacga gggtgaaggt tttgccggtt cctgtcgcca tggcgatcag catgctcttt
     1861 ttgccggcgc acagggcttt ttcgatggct tcgacggcgg caatctgata cggtcgcagg
     1921 cgggttattt cggcgacggg cctttgagcg aaccatcgtt cggcgctttc cgtgtcttta
     1981 cggtattttt cgagcagggc ccggggggag tggaaatcgg ccagcagccg ggacgtgttt
     2041 tcttttctgc gcacgtcgag atggaagatg gactctccgt tggtggaata gaggaaggga
     2101 accctgtacc ccctccattc gcctaccgtg tccggtacgc cgcgggcgta tcgcttggcc
     2161 tgttccagca cgttttcggc gccgagggag acttttttgg cttctatgat gccgagcagt
     2221 tttccgtccg caaaaagggc gtaatcggca gggccggtct cggtggggta ttcttccacg
     2281 gcgtgacggg tcagcgatga acagtcgcgg acttcgtcgc tgcggatgag agtccagccg
     2341 aggactgatg atcgaagccg cgcatcgatg cgttcttttc gcgtcctggc ctcggattca
     2401 gtcattgcca caccccttcg tctttgtgtg ctgcggagga cattccggca cgagcaagcg
     2461 ctctaaaaac cttctacgcg cgcagcttcg tagaaggtgg acgtcttgcg cattcccagc
     2521 atacggaacg catcccgaaa cagggtttgt ccttccaatg tgctggagaa gacggcacgg
     2581 gcgaagcgtt taccggtgcg tgcgcccagg gtgcggtaaa aatcaccgcc gccgccggtg
     2641 ccacgatcca gcgcccgtat tctttccacc tcttggcggt agttcagcca aagagtgtcc
     2701 ttatcgatga atccggcatc gaatagacgg cgcagcgcca caagggaact cactttaaac
     2761 aggtgggcca ggcgctgtat ctcgtcggca accggagcgt caatgtcgta tgcctcattc
     2821 agcgcatcca gcggcatcag cagttcggcg gccactctgt tgcaccaccg ttcaagccct
     2881 tgcttcggga cgctgccggc ttccggatcg gatacaccgc tttctcccga ccaaatatgg
     2941 gccagttcgt gggcgatcgt aaacatttga gcagccttgc tgtcggaggc gttaatgaag
     3001 atcagcgggg ctctgtcgtc aaccagagcg aaacctcgaa attcggtcgc gtccagtttt
     3061 cggtgactgt tgctgccgac gatagaactg gccattacaa gtattccggt gtcttcagcc
     3121 ctggaaatga gttgacgtaa ggcatcggtc cacgtcgaca gcctgcgccg atctgcgacg
     3181 gaaagcccca aaacctgccg catcgagtcg gccacctgct ccggctggac atcttcggat
     3241 gcactggaga caaattcgag gcgtgcaaaa ccatgtatgc gggcatactc gcgataccag
     3301 tcctgccgtt gttggcagag gtacagggta tccagcaacg cggcgctcgg ttcggccagg
     3361 gcttcatcgc gccgggtccg gaaatcgggt atcgggagtt gcagtgcggg cggttcaggc
     3421 agaaaaaaat acccgatcgg ggtgtgagta agcctggcga aatcttccag ctgtttcagc
     3481 gttggcaggg catctccaga gagccataaa tcccactttc gaaaagacct ctccaaagcg
     3541 gtatcggaca gatgagcccg ttccgctgcc cagcgcaata ctggaatgga tgcgggtacg
     3601 gtgttcatac gctcatctcc tttgcaaaaa tttgggcggt cagaggggct atcgaacgaa
     3661 accgccattc attcgacgca cgggggactt caggacaatt cgattcccgt tgtaccacag
     3721 cgcatcctgt tcagcaagat ttttcgatgc tttgaaaagg gtgctccacc cccgaatccg
     3781 cagtctctta agttcgtcac tcccggggga tccgctcggt taagatatct gcgtccaccc
     3841 cccatcttga ggtcacattt tgtgacctca agatttcgag ttcagttcga aagccgaaac
     3901 ataaattcgg gagggaatct gtcactgttc cgatcgcccg tttcggcagt ttgcttctcc
     3961 agtaaaaaat agacgccggg acgctgtgct tccacccaat ccctgagttt attcaaacgg
     4021 ctgcgagggc aggcaattgc ctgtcctgac cagttcgcta tctcgacgtg ccgtatgccg
     4081 gagggggcac
//
LOCUS       DAOPKU010000250         4087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24102_length_4087_cov_4.825149, whole genome shotgun sequence.
ACCESSION   DAOPKU010000250 DAOPKU010000000
VERSION     DAOPKU010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24102_length_4087_cov_4.825149"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /gene="hcp"
                     /locus_tag="PK849_08560"
                     /gene_synonym="priS"
     CDS             complement(<1..963)
                     /gene="hcp"
                     /locus_tag="PK849_08560"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPE66215.1"
                     /translation="MYCFQCQETAKNQGCTVKGVCGKPEGTANLQDLLIYVCKGIAVY
                     GEKLKQQGTVDREAGRFIIRALFVTITNAAWDDDSIIEWIRKGLKVKDAVAQKAGAAG
                     NLPDCATWKAESKEEILAKAMSAEVRVTAAQNEDVRSLRELVIIGGKGVAAYAEHAEV
                     LGFEKDEIYSALMEGLASTTKDLSTDEMVGMVMKIGETAVTTMALLDEANTSTYGNPE
                     ITEVNLGVRNNPGILISGHDLKDMEELLKQTEGTGVDVYTHGEMLPANYYPAFKKYEH
                     FVGNYGGSWWHQNEEFESFNGPVFLTTNCLVPVKKENTYLGRLFT"
     gene            complement(1041..1820)
                     /locus_tag="PK849_08565"
     CDS             complement(1041..1820)
                     /locus_tag="PK849_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE66216.1"
                     /translation="MNTIMRKIIKIDEEKCDGCGICAEACHEGAIRIIDGKAKLVSET
                     YCDGLGDCIGECPQGAISFEMREAAAYDEEAVKRHMAAKAAGKSSADPLPCGCPGSAA
                     RDLRAAPSATLQARPEAAPAPLQSELVNWPVQIRLVPETAPWLHKAELVIGADCVPFA
                     FPDFHRTFLAGGDTICLIGCPKLDDAQSYVEKLTRIISYNETPAITVVRMEVPCCGGM
                     TRILEAACEASERNISLKIVTIGIRGDILDKETIRFSRRQP"
     gene            complement(1817..2227)
                     /locus_tag="PK849_08570"
     CDS             complement(1817..2227)
                     /locus_tag="PK849_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPE66217.1"
                     /translation="MSEVVAVSEAVSLAFHGMGLLASGERKSAKEMAEAVNVSEAHLA
                     KVFQRLVREGLVGSTRGPKGGFELAKRPDEITLFDIYIAIEGVPKGDYCLLHKQQCPF
                     GRCIFGSLLRNLTREFVDYLKNTTLGDLQRGEAE"
     gene            complement(2432..3853)
                     /locus_tag="PK849_08575"
     CDS             complement(2432..3853)
                     /locus_tag="PK849_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HPE66218.1"
                     /translation="MEANTVLAGRILELVGGKGNVSSAANCMTRIRMRLKDASKADVA
                     GLKATDGVLGVVEGATLHVIVGPGKAKKVADILVEEFGVARDSAVTEDWEENKAAVKQ
                     GQKKGPLKTALETIAGIFIPLIPAIIAAGIFNGVGSLLATMLKQQAIAGPGWEFARLI
                     CQLGGSAFLGYFAIFTGVNAAKRFGATEALGGMVGAMTVGAQINEISQLLGLFDKDVP
                     LNSILITGKGGIIGVILGVYILAKVEKRVRKLVPDVLDLVFTSLITLLIVALGMVFVI
                     MPVSGYFSDWLVEALGLIINSDNTVVSIISGYVLAALFLPMVLLGLHHGLIPIYAIQL
                     QNFGGVSLFPVLAMAGAGQVGAAVAIWLKARKVKNTRIQSVIAGALPAGVLGIGEPLI
                     YGVTLPLGKPFITAGLGAGFGGAYVMATGVMATSWGPSGLVALPLMQPGSIMNYFVGI
                     LISYVGGFIITSLFIKESDVANV"
BASE COUNT          726 a         1300 c         1245 g          816 t
ORIGIN      
        1 ggtgaacagc ctgccgaggt aggtgttttc cttcttcacg gggaccaggc agttggtggt
       61 aaggaagacg ggtccgttga aggactcgaa ctcctcgttc tggtgccacc aggagccgcc
      121 gtaattcccc acgaagtgct cgtacttctt gaacgcgggg tagtagttcg cgggcagcat
      181 ctcgccgtgg gtgtacacgt ccacgccggt accctcggtc tgcttcagaa gctcttccat
      241 atccttcagg tcgtggccgg agatgaggat tcccgggttg ttcctgacgc cgagattgac
      301 ttcggtgatt tccgggttgc cgtaggtgct cgtattcgcc tcgtcgagaa gcgccatcgt
      361 ggtgacggcg gtctcgccga tcttcatgac catgccgacc atctcgtcgg tggagaggtc
      421 cttcgtggtg gaagcaagcc cttccatgag cgccgaatag atctcgtcct tctcgaagcc
      481 gaggacctcg gcgtgctcgg cgtaggccgc gacgcccttg ccgccgatga tgacgagttc
      541 ccgcagcgag cggacgtctt cgttctgcgc ggcggtgacg cgaacctcgg cggacatggc
      601 cttcgcgagg atctcttcct tgctctcggc cttccacgtt gcgcaatcgg gaaggtttcc
      661 ggcggcgccg gccttctgcg cgaccgcgtc cttgaccttc agccccttgc ggatccactc
      721 aatgatggag tcgtcgtccc acgccgcatt ggtgatggtg acgaaaagcg cgcgaatgat
      781 gaaacggccg gcctctctgt ccaccgtccc ctgctgcttc agcttttcgc cgtatacggc
      841 gatgccctta cagacgtaga tgagaaggtc ctgaagattg gcggtgccct ccggctttcc
      901 gcagactccc tttactgtgc acccctgatt cttcgctgtc tcctgacact gaaaacaata
      961 catgccggcg cttcctcctt tgtgaatttg attttttcga aacatgccga gtacccgttg
     1021 atacgtctcc gatagtgcgc tcatggctgt ctcctggaga acctgatggt ctccttgtcc
     1081 agtatatcac cacgaatacc tatcgtcaca attttcagcg atatgttccg ctccgaggcc
     1141 tcgcatgccg cctccagaat gcgcgtcatc ccgccgcagc aggggacttc catgcgcacc
     1201 acggtgatcg cgggcgtttc gttgtatgag ataatccgcg tgagcttttc gacgtagctc
     1261 tgcgcgtcgt cgagcttggg gcagccgatg aggcagatcg tatcgccccc ggcgaggaag
     1321 gttctgtgga agtccgggaa ggcgaacgga acgcagtcgg cgccgatcac aagttccgcc
     1381 ttgtgcagcc agggcgcggt ctccggcacg aggcgtatct gcaccggcca gttcacaagc
     1441 tccgactgaa gaggcgcggg cgccgcttcg gggcgcgcct gcagagtcgc ggacggcgcg
     1501 gcgcgcagat cgcgggccgc cgaaccgggg cagccgcagg gaagaggatc ggcggagctt
     1561 ttgcccgcgg cctttgccgc catgtggcgc ttcacagcct cttcgtcgta cgccgcggcc
     1621 tctctcattt cgaagctgat ggcgccctgc ggacactcgc cgatgcagtc gcccagcccg
     1681 tcgcagtacg tttcgctcac cagcttcgcc ttgccgtcga tgatccggat tgcgccctcg
     1741 tggcaggcct cggcgcagat gccgcagccg tcgcacttct cctcgtctat cttgatgatc
     1801 tttctcataa tcgtgttcat tccgcttctc ccctctgcaa atctccaagc gttgtgttct
     1861 tcaggtagtc gacgaactct ctcgttaaat ttctaagcag cgaaccgaag atgcaccgtc
     1921 cgaacgggca ctgctgcttg tggagcaggc agtagtctcc ctttggaact ccctcgattg
     1981 cgatgtagat atcgaagagg gtgatctcgt ccggccgttt cgccagctcg aaaccgccct
     2041 tcggcccgcg cgtggagcct accagccctt cgcgcaccag cctctggaag accttggcga
     2101 ggtgggcttc cgagacgttc accgcctcgg ccatctcttt cgcgctcttt ctctcccccg
     2161 aagcaagcag tcccattccg tgaaaagcca gggacaccgc ctcggacaca gcaaccacct
     2221 cgctcatggc ctcgactccc tttctcattt ctggtattat ggtactgtaa ttcctgaata
     2281 tgtcaagagg ggatttcgaa aagttttcga agaagatgtg aaagaaggag tacggcggct
     2341 gaggatgaaa acgcccgccc ggcggtatcg gccgggcggg cgctggaaag cggcgccgtt
     2401 cagaagagaa gcggggccgc gtgcatgtcc gttacacgtt ggcgacgtcg gactccttga
     2461 tgaagagcga cgtgatgatg aatccgccga cgtaggagat caggatgccg acgaagtagt
     2521 tcatgatgct gcccggctgc atcagcggaa gcgcgaccag cccggacggc ccccaggagg
     2581 tggccatgac gcccgtcgcc atgacgtacg cgccgccgaa gcccgcgccc agtccggcgg
     2641 tgatgaacgg cttgcccaga gggagcgtga cgccgtagat caagggctcg ccgatgccga
     2701 gcacgcccgc ggggagcgcg cccgcgataa cgctctggat acgcgtgttc ttcactttcc
     2761 gtgctttcag ccagatggcg accgccgcgc cgacctgccc cgccccggcc atcgcgagca
     2821 cagggaagag ggagacgccg ccgaagttct ggagctggat ggcgtagatg gggatgagcc
     2881 cgtggtgcag ccccagcagg accatgggaa ggaagagcgc ggcgaggacg tagccggaga
     2941 taatgctcac gacggtgttg tcggagttga tgatcaggcc gagcgcctcg accagccagt
     3001 cggagaagta gccggagacg ggcatgatga cgaagaccat ccccagcgcg acgatcagga
     3061 gcgtgatcag ggaggtgaag acgaggtcga gtacgtcggg aacaagcttg cgcacgcgct
     3121 tctcgacctt ggcgagaatg tacacgccga ggataacgcc gatgatgccg cccttgccgg
     3181 tgatgaggat ggagttcagc ggaacgtcct tgtcgaagag tccgaggagc tgcgagatct
     3241 cgttgatctg cgccccgacc gtcatggcgc ccaccatgcc gccgagagcc tcggtcgcgc
     3301 cgaagcgctt cgccgcgttg acgccggtga agatggcgaa gtagccgagg aaggccgagc
     3361 ctccaagctg acagatcagg cgggcgaact cccacccggg ccccgcgatc gcctgctgct
     3421 tcagcatggt ggcgaggagg ctgcccacgc cgttgaagat gcccgccgcg atgatcgccg
     3481 gaatgagggg gatgaagatg cccgcgatgg tttcaagggc cgtcttgagc ggtcccttct
     3541 tctgcccctg cttgaccgcg gccttgttct cctcccagtc ctcggtcacg gcgctgtcgc
     3601 gcgcgacgcc gaactcctcg acaagaatgt ccgcgacctt cttcgccttg ccggggccga
     3661 cgatgacatg aagcgtcgcc ccctccacca cgccgagcac cccgtcggtt gccttcagtc
     3721 cggcgacgtc cgccttcgag gcgtccttca ggcgcatgcg aatccgcgtc atgcagtttg
     3781 ccgccgagga gacgttcccc ttgccgccga caagctccag aatcctgccg gccaataccg
     3841 tgttcgcttc cattaagtca cacacctctc ctgttgaaat accgcgctcc gcgtctttgc
     3901 gaaaagaccg tcccactgcg gcggctcttt gcgcaacgac gcggagctgc gggaaaaact
     3961 cactgcagtg cgagccgcac gcgccccttc gccgcctcca gcttcgcccg agcctcgtcg
     4021 agcgggcagc cgagcagcag catgacgata gcgtgcttca cgctcccctc cgccttttcg
     4081 aggaact
//
LOCUS       DAOPKU010000251         4085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24127_length_4085_cov_4.633251, whole genome shotgun sequence.
ACCESSION   DAOPKU010000251 DAOPKU010000000
VERSION     DAOPKU010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24127_length_4085_cov_4.633251"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1989)
                     /locus_tag="PK849_08580"
     CDS             complement(202..1989)
                     /locus_tag="PK849_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HPE66219.1"
                     /translation="MKSLTLKTRLWILALIPVFGILSVTGVLMYSANAIYNDLLAQIH
                     QEAFVAQSLVLNGDRDLYQGLAAKQSILQGGAGKDFDKNLKDFRENVAQVTERLGNAE
                     KLLARNKAAWEPFRLEGKTESVFDKFNTLNKDFPAWIKESEQQLDDIRTGKLAHGSVQ
                     QIEDPLFKKVRGNINEIGEILDIGAENSALANKQRMTAANISMGAVAIFVALLAVVIA
                     FTTIRKIRRSVASILAASKAGKEGNLTVRTSMTGDDELAAVGHSLDAMLDSLREVISE
                     LQKKSVVLSTLSENTAASCEEVTSTTNEVAESNSKLAEEVSLGRSSAVDASRNILDMN
                     TVIQSAQAVASRANENSREMAEAAAKGRETVASSIGHMEGIRDAVADTGKTISQLNQY
                     SQRIGVVGTTITSLADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEQSQQGAR
                     EVAELVERILRGTSDAVTSMEKSLHDVEEGVAIAHVAGDALEKIMTATKSSVDDIRLI
                     IEATEQEADKAEVVVALIDRTSSVMENADMQVQTVAASMEETAAAMENVASGATEVNS
                     TAEDLRRLAERFIVDGESGGQLALPGRKS"
     gene            2170..2961
                     /locus_tag="PK849_08585"
     CDS             2170..2961
                     /locus_tag="PK849_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPE66220.1"
                     /translation="MHFGLEGKTVLVLASGSGLGKAVAGEFLKEGADVVLAGRGAEKL
                     ERAAADLRGATGITPRFQQCDITKAEELHGLVRFAAQKHGSVDVLVNNAGGPPAGTFE
                     IFDDAAWTGAFELTLLSYVRAIREVLPYMKQRGWGRIVNSTSSSVREAIENLILSNTF
                     RLGVIGLTKSLAAEFAPYGILVNAIGPGRFDTERIRSLDRSLAEKKGLSVEEVSRASL
                     SRIPMGRYGDPEEYGRLAVFLGSEANTYITGQTILADGGMTRAVP"
     gene            complement(3098..>4085)
                     /locus_tag="PK849_08590"
     CDS             complement(3098..>4085)
                     /locus_tag="PK849_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPE66221.1"
                     /translation="TACGSMGYAMLDARYADHVIAVTDNLAEYPLAPRISVPQYLVDQ
                     VVVVDSIGDPSKIATGATRVTRDPVQLRIAELAFGLLKASGTLKNGCSFQTGGGGPSL
                     AVARYVKEYMKSHAIKGSYCIGGITGYMASMLEEGLFDALFDVQSFDAAVTSSIANNP
                     RHIEVDVSCYANPFNKGCLVNDLDVVILAALDVDTDFNVDVLTGHDGVLRGASGGHSD
                     TAAGAKLTVITIPSMRNGVPSIRNRVQTVVTPGETVDVIVTERGMAINPLREDLRALA
                     TDAGLPVKDIKQLKAEIEKVTGIPEEVRFGDEIVGLVEYRDGTIIDTIRKVL"
BASE COUNT          699 a         1252 c         1283 g          851 t
ORIGIN      
        1 agccgtaccc tgtgcggatc accgtgcaga gccagctctt cggtatgctg gtggagatcg
       61 acgccattgc cgagttggac tgagaacgac ggatcggatg cctgagtccg cgttcaccag
      121 cgttctgtaa agagacacgc aaacgccccc tcgcagcgag ggggcgtttg tcaaactcat
      181 ccggcgtgtg cgtttttccc gtcaggattt tctcccggga agggcgagct gcccgccgct
      241 ttccccgtcg acgatgaatc gctccgcaag cctgcgcagg tcctccgcgg tgctgtttac
      301 ctcggtggcg ccgctcgcga cgttctccat ggccgccgca gtctcttcca tggaggcagc
      361 cacggtctgc acctgcatat ccgcgttctc catcaccgac gatgttctgt cgatcaacgc
      421 gacgacgact tccgccttgt cggcctcctg ttccgtggct tcgatgatca gccggatgtc
      481 gtccacggag cttttcgtcg ccgtcatgat cttctccagc gcgtctcccg cgacgtgcgc
      541 aatggcgacc ccctcttcga cgtcgtgcag gctcttctcc atggaggtca ccgcgtcgga
      601 ggttccccgg aggatccgtt ccacaagctc cgcaacttcc cgcgctccct gctgcgactg
      661 ctcggcgagc ttgcgcacct cgtcggcgac gacggcgaat cctcgtccgg cctcgcccgc
      721 tcgggcagcc tcgatggccg cgttcagcgc gaggaggttg gtctggtccg cgagcgaggt
      781 gatggtcgta cccacgacgc cgattctctg agaatactgg ttgagttgcg agatggtctt
      841 ccctgtgtcc gcgacggcat cccggattcc ctccatgtga ccgatggagg aggcgaccgt
      901 ctcccttccc ttcgccgccg cttccgccat ttcgcgggag ttttcgttgg cccgcgaggc
      961 gaccgcttgt gcggactgga tgacggtgtt catatcgagg atgttgcgcg aggcgtcgac
     1021 ggcgctgctg cgccccagac tgacctcttc ggcaagcttg ctgttgctct ccgcgacctc
     1081 gttggtggtg ctggtgacct cctcgcagga agcggccgtg ttctccgaga gcgtggaaag
     1141 gacgaccgat ttcttctgaa gctcggagat aacctcgcgg aggctgtcga gcatggcgtc
     1201 gagcgaatgg ccaacggccg caagttcgtc gtctcccgtc atggaggtcc ggactgtcag
     1261 attcccctcc ttaccggcct tcgatgccgc gagaatggaa gcgaccgaac ggcgtatctt
     1321 ccggatggtg gtgaacgcga tgacaactgc caaaagcgcg acgaaaatcg ccaccgcgcc
     1381 catggagata ttcgccgccg tcatacgctg tttgttcgcc agcgcgctgt tttcggcgcc
     1441 gatgtccagg atttcgccga tctcgttgat gttcccccgg acttttttga acagaggatc
     1501 ctcgatctgc tgcacggagc cgtgcgcaag ctttcccgtc cggatatcgt cgagctgttg
     1561 ttccgactct ttaatccacg ccgggaagtc cttgttcagc gtattgaact tgtcgaagac
     1621 ggattccgtt ttcccctcaa gccggaacgg ttcccacgcg gccttgttgc gtgcaaggag
     1681 tttttcggcg ttgccaagcc gctccgtgac ctgcgccacg ttctcccgga aatccttgag
     1741 gtttttgtca aagtcctttc cggcgccccc ttgcagtatg gattgcttcg ccgcgagccc
     1801 ctggtacagg tcgcggtccc cgttgagcac caggctctgc gccacgaagg cttcctggtg
     1861 aatctgggcg agaaggtcgt tgtagatggc gttcgccgag tacatcaaga cgcctgtcac
     1921 actaagtatg ccgaaaaccg ggataagcgc gagaatccat aatctcgttt tcaacgtcaa
     1981 gctcttcatt attctccgct cctttcagtg agggcacttt tttcacagga tggtacagga
     2041 ttttttatat tctacaccgg actcggggat tccggatagc ccccaggggg taaacgccgt
     2101 gagatgtccg cgaagaaggt gtaaaataaa aaaagttttc aagagcagta ttcgaaggag
     2161 gggtgactga tgcacttcgg actggaagga aagaccgtgc ttgtcttagc ctcgggttcc
     2221 gggctcggga aggccgtggc cggagagttc ctgaaggagg gagcggacgt ggtgctcgcc
     2281 ggaaggggag cggagaagct cgaaagagcg gcggccgact tgcgcggggc gaccggaatc
     2341 acgcctcgtt ttcagcagtg cgacatcacg aaggccgagg agctgcacgg gttggtgcgc
     2401 ttcgccgcgc agaagcacgg cagcgtggac gtgctggtga acaatgcggg agggcctccc
     2461 gccggaacgt tcgagatatt cgatgacgca gcctggacgg gagctttcga gctgacgctg
     2521 ctgagctacg tgcgggcgat ccgcgaggtg cttccgtata tgaaacaacg tggttggggc
     2581 cgcatcgtca actcgacgtc ctcttcggtg cgggaggcta tagagaacct cattctctcc
     2641 aatacgttcc ggctcggtgt gatcgggctg acgaagagcc tcgccgccga gttcgcccct
     2701 tacggcatcc tcgtgaacgc catcggcccg gggcgcttcg acacggagcg catccggtcg
     2761 ctcgaccgct cgctggccga gaagaaggga ctgtccgtcg aggaggtaag cagagcgtcg
     2821 ctctcgcgaa ttccgatggg acggtacggc gaccccgagg aatacggacg gctcgccgtt
     2881 ttcctcgggt cggaggcgaa cacctacatc accgggcaga cgatcctcgc cgacggcggc
     2941 atgacgaggg cggtgccgta gaggcggcaa cagagacagt cccctttgcc gaaccggggc
     3001 ctcagttgaa agaagcagga aaggccgagg ggccttcgcc gcgtgcggcg atttcccccg
     3061 gccttttgat tgttgagaaa gtaccttttt ctgcgggcta cagcactttc cgtatcgtgt
     3121 cgatgatcgt gccgtcccgg tactccacaa ggcccacgat ctcgtcgccg aagcggacct
     3181 cttcggggat gccggtgacc ttctcgatct ccgctttcag ctgctttatg tccttcacgg
     3241 gaagccccgc gtccgtggcg agagcccgaa ggtcctcccg gagcgggttg atggccattc
     3301 cccgttcggt aacgatgacg tccacagtct ctccaggcgt cacgactgtc tgcacacggt
     3361 tccgtatcga gggcacgccg ttgcgcatcg aagggatggt gatcacggtg agcttcgcac
     3421 cggcggccgt atcggagtgc ccgccggacg cgcccctgag gacgccgtcg tgccctgtga
     3481 gcacgtcgac gttgaagtcc gtgtccacgt cgagcgcggc gagaatgacc acgtcgaggt
     3541 cgttcacaag gcaccctttg ttgaaggggt tggcgtagca ggagacgtcc acttcgatgt
     3601 gccgcgggtt gttcgcgatc gacgaggtca cggccgcgtc gaagctctgc acgtcgaaga
     3661 gggcgtcgaa gagcccctct tccagcatgg acgccatata gccggtgatg ccgccgatgc
     3721 agtagctccc cttgattgcg tgcgatttca tgtactcctt cacgtagcgc gccaccgcga
     3781 ggcttgggcc gccgccgccg gtctggaagg agcagccgtt cttcagcgtc cccgaggcct
     3841 tgaggagccc gaacgccagc tccgcgatcc tgagctgcac ggggtcgcgg gttacgcggg
     3901 tggccccggt ggcgatcttt gagggatcgc cgatggagtc gaccacgacc acctgatcca
     3961 ccaggtactg cggaaccgag atgcgcggcg cgagcgggta ctccgcgagg ttgtcggtga
     4021 cggcgatcac gtgatccgcg tagcgcgcgt cgagcatcgc gtaccccatc gagccgcagg
     4081 ccgtc
//
LOCUS       DAOPKU010000252         4077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24190_length_4077_cov_5.017156, whole genome shotgun sequence.
ACCESSION   DAOPKU010000252 DAOPKU010000000
VERSION     DAOPKU010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24190_length_4077_cov_5.017156"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK849_08595"
     CDS             complement(<1..438)
                     /locus_tag="PK849_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HPE66222.1"
                     /translation="MKSLGDMLLNTGPFSEIVLGNTALARAMIESGTEVIASYPGSPT
                     PEIGAALMSVPKERRPYYFEFSTNEKVATEVACGASLNGRPSCVFFKSVGLNVAADSF
                     VQLSLLELNGGLVAILGDDPGANSSQNEQDNRHIARLAYIPVLE"
     gene            complement(456..638)
                     /locus_tag="PK849_08600"
     CDS             complement(456..638)
                     /locus_tag="PK849_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66223.1"
                     /translation="MESGDPLDSDEFLPLKDESGKVTILVPKKMKFTDDKVLVEHFTY
                     RGKDVFICRTALLPDR"
     gene            complement(660..827)
                     /locus_tag="PK849_08605"
     CDS             complement(660..827)
                     /locus_tag="PK849_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66224.1"
                     /translation="MKKRIVLLGIAALLMVFSLSAAAQELHLALEQSALEKLSAKEGA
                     LYIEWGVYRNT"
     gene            1071..3884
                     /locus_tag="PK849_08610"
     CDS             1071..3884
                     /locus_tag="PK849_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPE66225.1"
                     /translation="MLFLPDSISFPERKPKVWIWDDLYRALAEALGVKPRVQIDPPDH
                     WLLVRHIVRRLRDRCAGALPAGTASPRFLELAGQSIRELLREAVPREELAKALGCGGC
                     APGTRCERLGDERAASRNERASDAGSALLCALYGDYIALLEERGLADSAQIPSLGAAL
                     LEAFPQEGRAWAGGLKLRAAGFLSFASGQMAFLHSLCAAGADLELWVPECGEGDFYTA
                     VQQFPEAELLRCSGESDAVPPLCVSMAAGDLRLSTDTLARELFLWPAGEGYLSAASGL
                     SFPGWENIALCAESEELNSLVESFTRYGIPYSLREGATVSETALWKSALRALDLAADG
                     WPPVETADFLSSLLFAPFSFPRRAFGNALPSGKKGWTGFLEEFPPEAGRDAFRRATAF
                     ADVVQRGGRPEELLSAFAHLAPSRAELKLLVERAARFPRLDEHIREVSLAVQEANEKE
                     RSLRELRRDLGDAGSARLSFEEAVAFLGQWAETASIWMPSPVSPTVAIHPGTPPSLVR
                     APVWILPGMTAKRWPGQVHESPLLSDERKEFLHESLELGRSHLPLVPEKRSQREALFR
                     RLAACGESLCVLLRPLADDGGRPLPESPFVDDACSSSHPWLRSSGAEPLKRSLGEILA
                     PEEYPVAEGVETRWRSWATTGAARGSLKEIPLLPPKEKVFSLSDLDTYVSCPFRYYCA
                     RTASLDTPPEELHRFDLAGSALHLLWQKVWERRLSTGEQLDFLVNGLFDEAMDEKYAR
                     LLASENLKRDRKELLRRAVRLARVQQTMEDGGLASGRLEQKREYTLPEFERGGVRFKG
                     RCDRVDFLKGGTAAIFDYKSGPSSNYKESLQLAAYGIALAASGIRTVASAFLCLADGK
                     EKGASVEGAPSVFASFTTPLAPLESDAEEALERVAESFATGLFPPLYESKSCRFCAYS
                     SLCRRRDFHSAESEEEGEENGGR"
     gene            3871..>4077
                     /locus_tag="PK849_08615"
     CDS             3871..>4077
                     /locus_tag="PK849_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPE66226.1"
                     /translation="MAGGDLLARLKAHIASEGALLPAQEEAVFSDAALTLVGAGAGTG
                     KTHTLSWRFIRALLREDVRPRDILT"
BASE COUNT          641 a         1317 c         1391 g          728 t
ORIGIN      
        1 ttcgagcacg gggatgtagg cgaggcgggc gatgtggcgg ttgtcctgct cgttctgcga
       61 ggaattggct ccgggatcgt cgccgaggat ggcgaccagg ccgccgttca gctccagtag
      121 cgagagctgg acgaaggagt cggccgcgac gttgagaccg acgcttttga aaaagacgca
      181 cgaggggcgc ccattgagcg acgcgccgca cgcgacctcc gtggccacct tctcgttggt
      241 ggagaactcg aagtagtagg gacggcgctc tttgggaacg ctcatgagcg cggccccgat
      301 ctccggcgtg ggggagccgg gataggaggc gataacctcc gtcccggact cgatcattgc
      361 gcgggccagc gcggtgttcc cgaggactat ttcactgaac ggcccggtat tcagcagcat
      421 gtcgccgaga cttttcattt gaggacctcc ttcgtttacc ggtcgggcag gagagccgtc
      481 ctgcagatga aaacatcctt tccgcgatag gtgaagtgct ccacaagaac cttatcgtcg
      541 gtgaacttca tcttcttggg cacaagaatg gtcaccttgc cggattcgtc cttcagcgga
      601 aggaattcat cggagtcgag gggatcgccg ctctccacga gagcggatgg cttctcccct
      661 caggtgttgc ggtagacgcc ccattcgatg tagagcgctc cttctttcgc cgacaacttt
      721 tcgagcgcgc tctgctccag cgcaaggtga agctcctgcg ctgccgccga cagagagaac
      781 accatcagca acgccgctat acccagcaat acgatacgtt ttttcatgaa cgaagatctc
      841 ctcctcttat aaaatgaaac agattctctc cgggaattgt atcacggact ccgtgttgag
      901 tgctacactt atcgcgagcc gcgaaatgat cgcacgagga ggcgttcccc ctgagactcc
      961 acacctaccg caccccccga agccttgcag aaccgctgcg cgaagaggcc gcgcgcggcg
     1021 acgcgatctt tctgacgccg tcgaacaggg accgtccgct gctgctggag atgctcttcc
     1081 ttccggactc gatctccttc ccggagcgga agccgaaggt gtggatatgg gacgacctct
     1141 accgggcgct cgccgaggcc cttggcgtga aaccccgcgt gcagatcgac cctcccgacc
     1201 actggctgct cgtccggcac atcgtccgtc ggcttcggga ccgctgcgcc ggcgcgctgc
     1261 ccgccgggac ggcctctccg cgcttcttgg agctcgcggg acagtccatc cgcgaactgc
     1321 tcagggaagc cgtgccgagg gaggagctgg cgaaggcgct ggggtgcggc ggctgtgctc
     1381 cgggtacgcg gtgcgaacgt ctgggcgacg aacgtgcagc ctcccggaac gaacgtgcga
     1441 gcgacgcggg gagcgcgctt ctttgcgcgc tctacggcga ctatatcgct ctcctcgaag
     1501 aacgcggcct cgccgacagc gcgcagatcc cctcgctggg agcggcgctg ctggaggcgt
     1561 ttccgcagga ggggcgcgca tgggcgggcg ggctgaagct ccgcgccgca ggattcctga
     1621 gcttcgcttc ggggcagatg gcgtttctgc actcgctgtg cgctgcggga gcggacttgg
     1681 agctctgggt tcccgagtgc ggcgaaggag acttttacac cgcggtgcag cagttccccg
     1741 aggctgaact tctccgctgt tccggagaaa gcgacgctgt gccgcctctc tgcgtctcca
     1801 tggcggcggg cgacctccgc ctctcgacgg acacgctcgc tcgcgagctc tttctctggc
     1861 ctgcgggcga ggggtatctc tccgcggcga gcggcctctc ctttccgggg tgggagaaca
     1921 tcgccctctg cgcggagagc gaggagctga actccctcgt cgagagcttc acccggtacg
     1981 gtattcccta ctcgctgcgc gagggagcga cggtctccga aacggcgctc tggaagagcg
     2041 cgctgcgggc gctcgacctg gccgcggacg ggtggccccc cgtggagacg gcggactttc
     2101 tttcgtcgct gctcttcgct ccgttcagct tcccgcgacg ggcattcggg aatgcgctgc
     2161 cttcgggcaa aaaagggtgg acgggcttcc tggaggaatt cccccccgag gccgggaggg
     2221 acgcgttccg ccgggcgacg gccttcgccg acgtcgtgca gcgcggcggg cgccctgagg
     2281 agctcctgtc ggccttcgcg caccttgcgc cctcccgcgc ggagctgaag ctgctcgtcg
     2341 aacgggcggc gcgcttcccc cgcctcgacg agcatatccg cgaggtgtcg ctcgccgtgc
     2401 aggaggcgaa cgaaaaggag cgttccctgc gcgaactgcg cagagacctt ggcgacgcgg
     2461 gaagcgcgcg tctctctttc gaggaggctg tggccttcct cgggcagtgg gcggagacgg
     2521 cgtcgatctg gatgccttcg ccggtctctc cgaccgtcgc cattcacccc ggcacgcccc
     2581 cctcgctcgt ccgcgcccct gtgtggattc ttcccgggat gacggcgaag cggtggccgg
     2641 gtcaggtgca cgaatcccct cttctgagcg acgaacggaa agagttcctg cacgaaagcc
     2701 ttgagctcgg gcgaagccac ctgccgctgg tgccggagaa gcggagccag cgcgaggcgc
     2761 tcttccgccg ccttgcggcc tgcggcgaga gcctctgcgt tctgctgcgc cctctggccg
     2821 acgacggcgg ccgtccgctc cccgaatccc ccttcgtcga cgacgcctgc tcttcgtcgc
     2881 atccgtggct gcgctcttcg ggagcggagc cgctgaaacg ctcgctcggc gagattctcg
     2941 ccccggagga gtaccctgtg gcggagggcg tggagacgcg ctggcgctcg tgggcgacga
     3001 ccggcgcggc ccggggcagc ctgaaagaga ttcccctcct ccctccgaag gagaaggtgt
     3061 tttcactgag cgacctggac acgtacgttt catgcccctt ccgttactac tgcgcccgca
     3121 cggcgtcgct ggacacgccc cccgaggagc tgcaccggtt cgatctcgcc gggagcgcgc
     3181 tccatctgct gtggcagaag gtgtgggagc ggcgcctttc gaccggggag cagctggact
     3241 tcctggtcaa tggacttttc gacgaggcga tggacgagaa gtacgcccgc ctcctggcga
     3301 gcgagaacct caagcgcgac cggaaggagc tgctgcggcg cgcagttcgc ctggcgcgcg
     3361 tgcagcagac gatggaggac ggcgggctgg cctccgggag gctggagcag aagcgggagt
     3421 acacgcttcc ggagttcgag agggggggcg tccgcttcaa ggggcgctgc gaccgggtcg
     3481 acttcctgaa gggcggcacg gcggccatct tcgactacaa gagcggaccg tcgtcgaact
     3541 ataaagaaag cctccagctc gcagcctacg ggatcgccct cgccgcgagc ggcatccgga
     3601 ctgtcgcgtc ggcgttcctc tgccttgcgg acggaaagga gaagggggcc tccgtggagg
     3661 gcgcgccgtc cgtcttcgcg tcgttcacca cgccgctcgc gccgctcgag tcggacgcgg
     3721 aggaggcgct cgaacgggtg gcggagtcgt ttgccacagg gctgttcccc ccgctgtacg
     3781 agtcgaaaag ctgccgtttc tgcgcctatt cgtcgctctg ccggaggcgc gacttccata
     3841 gcgcggaatc cgaggaggag ggtgaagaga atggcgggcg gtgatctgct ggcgcgtctg
     3901 aaggcgcata tcgcgtcgga aggggctctc cttccggccc aggaggaggc cgtcttctcg
     3961 gacgcggcgc tgaccctcgt cggcgcgggc gcggggacgg gaaagacgca tacgctttcg
     4021 tggcgcttta tcagggcgct gctccgcgag gatgtgcgcc cccgggacat cctgacg
//
LOCUS       DAOPKU010000253         4064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24300_length_4064_cov_4.436019, whole genome shotgun sequence.
ACCESSION   DAOPKU010000253 DAOPKU010000000
VERSION     DAOPKU010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24300_length_4064_cov_4.436019"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PK849_08620"
     CDS             <1..408
                     /locus_tag="PK849_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE66227.1"
                     /translation="SKADLNRVITRRHVQSHQNTPFSGRIAAEVIGAIRDEGLVERSE
                     RMGEYLLSGLREIQERFLCIREVRGRGLMVGAELTPEAASRGAGMSKRLVEKGFLQDF
                     HQMTSTFRLYPPFVITKDEIENFFEAFENVLAE"
     gene            complement(535..1437)
                     /gene="aroE"
                     /locus_tag="PK849_08625"
     CDS             complement(535..1437)
                     /gene="aroE"
                     /locus_tag="PK849_08625"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPE66228.1"
                     /translation="MKNETALQQSHALGTVEIRKNVERLVGLLGDPVAHTLSPAMQNA
                     AFQALGAGYRYLAFRVRAEDFEHAMEGLAALGAVGTNITVPHKERAFRWARRLDPSAS
                     RTGAVNTVLFSEEGGTGYNTDVAGIRTALAALSPRRDGALILGAGGAARAAVCAMQDE
                     GFSHIAIMNRTRERAEKLQAQLFPAKSCKLPTCEILSFEEAPSFTPDLLVNTTSLGMH
                     NTPWPEELLDTIVSLAKGGVVLDLVYARGRETHLCDRAQQSGIPSVSGQEVLLHQGTA
                     AFKLFTGMAAPVEIMRAALQKGNS"
     gene            1857..2561
                     /locus_tag="PK849_08630"
     CDS             1857..2561
                     /locus_tag="PK849_08630"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPE66229.1"
                     /translation="MKNDTPVSSGNREFLEQYIPLLDFFAEVCGPEYELVLHDVSRPE
                     SSIVAIRNGQISGRTVGSTMSEYAPTFIKLIQSGGYAEDMVAHVDRTKDNRILESHTF
                     FIKDEKGELRGMICANHDVTSLIKLHDTLHEKIRMLNGLSKDLAERTNEDPPFEAILE
                     KESHSNLNGLMDVLIEQASSEFSVAPPQMSPEERIRFVIMLKTRHLFSMKGAVAKVAE
                     RLGVSEATVYRYLKKA"
     gene            2728..>4064
                     /locus_tag="PK849_08635"
     CDS             2728..>4064
                     /locus_tag="PK849_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE66230.1"
                     /translation="MRELMTGNEAIARGAWEAGCHVAAAYPGTPSTEILENVSRYKEI
                     YSEWSTNEKVSLEVVSGASMAGARAMCAMKHVGLNVAADPFFTMSYIGVNGGLVIVDA
                     DDPGLFSSQNEQDNRWYALHAKLPMLEPSDSQECLDFMKAAFDISERFDTPVLLRVTT
                     RICHSKSVVETGERMERLIREYTRNPAKNAMLPVFAKAKHYLVEKRLLDLREFNETSP
                     FISYVEGDKEIGVLTSGVGYQHSREALGDRVSYLKLGMTWPLPEKAIRDFASKVKNLW
                     IVEENDAFIEGAVRGMGIACSGKDALPLVDELSPALISKAILGTKPSSGPDTGIAPPN
                     RPPLLCAGCPHRGLLFAMSKKKDLVVMGDIGCYGLGSMPPLNVGESTICMGAGFSAVV
                     GFQKAQEKAGRSQKVFGILGDSTFFHSGITGLIDAVVNKSRGVFVILDNRITA"
BASE COUNT          915 a         1054 c         1173 g          922 t
ORIGIN      
        1 tccaaagcgg atctcaaccg tgtgatcacc cggcggcacg tccagtcgca tcagaacacg
       61 ccgttctccg ggagaatcgc tgcggaagtg atcggcgcaa ttcgcgacga aggcttggtt
      121 gaacggtccg agagaatggg agaatatctg ctttcagggc tccgggagat acaggaacgg
      181 ttcctctgta tccgtgaagt gcgcggcaga ggcctcatgg tcggcgcgga gctgacgccg
      241 gaggctgcct ccaggggcgc cggaatgtcg aaacgtcttg tggaaaaggg gtttctccag
      301 gactttcacc agatgacgtc tacgttccgc ctctaccctc cttttgtgat aacaaaagac
      361 gagatagaaa acttttttga ggccttcgaa aacgtgcttg ccgagtagat gcttcagcgc
      421 ccctctgttg caccttacgc ggaacatgct ctcttggtaa cagattgtcg cggaggcgat
      481 gtaagttcgt gagtgcgggg cattcgtaaa cgcctcgcac tcacgaaatg agctttatga
      541 atttcctttt tgcagggctg ccctcataat ctccactgga gcggccattc cggtgaacaa
      601 cttgaaggct gccgttcctt gatgaagcag tacttcctga ccggagacgg acggtatgcc
      661 agactgttgc gcgcgatcgc agagatgtgt ttcacgaccg cgtgcataca cgagatcgag
      721 gacgacgcct cccttcgcca gggaaacgat tgtgtcgagc agctcctccg gccagggagt
      781 attgtgcatt ccgagcgacg tcgtgttcac aagcagatcg ggagtgaaag agggagcttc
      841 ttcaaacgag agtatttcgc atgtcgggag tttgcagctc ttcgcgggga agagctgcgc
      901 ctgaagcttt tcagcccgtt cacgtgtgcg gttcataatc gcgatatgtg agaatccttc
      961 gtcttgcatc gcgcagacgg ccgcgcgcgc cgcgcccccg gcgccgagga tgagagcgcc
     1021 gtctcttcgg ggagagagtg cggcaagcgc cgtacggatg cccgcgacgt ctgtgttgta
     1081 gcctgtgccg ccctcttcgg aaaaaagcac cgtgttgacg gctcccgtcc tggatgcgga
     1141 gggatccaag cggcgcgccc agcggaatgc gcgttccttg tgaggtacgg tgatgttcgt
     1201 ccccacggcc ccaagagcgg ccagtccctc catcgcgtgt tcgaagtcct ctgcacgaac
     1261 ccggaatgca agatagcggt accctgcgcc gagagcctga aatgccgcat tctgcatggc
     1321 aggcgaaagg gtatgggcga ctgggtctcc aagcagcccg acaagacgct caacgttttt
     1381 tcggatttca acggttccca gcgcgtgtga ttgctggagt gcggtctcat tcttcatggc
     1441 tttctcctcc ttggcctacg ggccggacgt atactaaaaa agccggaacc cgaaaccggg
     1501 cccggccttt tgttttttct ctgtcgacgg ttttgcgcta catgactccg tggacgctct
     1561 ggctgcggga gaaccggttt ccatacgaat gctgaccgtc ggcgtaataa taccaacggt
     1621 cgtagctaaa ggaaaaaccg gaaacttcgc ggggtgaaag acgtctcaag atcatgaaaa
     1681 cctgctttct cccatcaagg gtgtctgaaa gttgcgggta ataataccct cctttcaggg
     1741 aggtgtcaat acacgcccgc tcaatttggc cgtaaaactt ctttacagaa tccgcaggac
     1801 gtgatatgaa ttttttatca caacgttgtg tattttttga agggaaggaa cgcttcatga
     1861 aaaatgatac gccggtctct tcggggaacc gtgagtttct tgagcagtat ataccccttc
     1921 tggatttttt cgcagaagtg tgcggtccgg agtacgaact cgtgctgcac gatgtgtcgc
     1981 gtcccgagtc gtccattgta gccatccgga atggccagat aagcggaaga acagtgggga
     2041 gcacaatgtc ggagtacgcg ccgacattta tcaaactcat ccaaagcggc ggatacgccg
     2101 aggatatggt ggcccatgtc gacaggacga aggataatcg aatccttgag tctcatacct
     2161 tttttataaa ggatgagaag ggggagttgc gcggcatgat atgcgcaaat catgatgtga
     2221 ccagccttat aaaacttcac gacacgctcc acgagaaaat caggatgctg aacggacttt
     2281 caaaagatct tgcggagcgg accaacgagg atccgccttt tgaagcgatc ctggagaaag
     2341 aatctcattc caacctgaac ggcctgatgg atgttcttat cgaacaggcc tcctcggaat
     2401 tttccgttgc tcctccccag atgagcccgg aagagagaat tcgatttgtg atcatgctca
     2461 aaacgcgcca cctcttctcg atgaaagggg cggtggcaaa ggtggcggaa cgtctcggcg
     2521 tttctgaagc gacggtttat cggtatctga agaaagcgtg agcatgctct ttattgaagg
     2581 ctgtgtaaag agcgttttgg agtagcgtct ctcgcccttc attcacacgt tgcaaaagaa
     2641 ggaaaggtgt gataaaaatt tattatcaca ccgtcgcttc ttctccgtga agaaggcggt
     2701 ctgttcggaa ggagtggagc gaagaaaatg agagaactga tgaccggaaa cgaggccatt
     2761 gccagagggg cctgggaagc ggggtgtcac gtggcagccg cctatccggg gaccccgagc
     2821 acggaaatcc tcgaaaatgt ctcccgctac aaggagatct attccgagtg gtccacaaac
     2881 gaaaaagttt ctctggaggt cgtctccgga gcgtcaatgg ctggggcccg ggcgatgtgc
     2941 gccatgaagc acgtgggatt gaacgtggcg gcggatcctt ttttcacgat gtcgtatatc
     3001 ggcgtcaacg gaggtctcgt catcgtcgac gcggacgacc ccggactttt cagctcgcag
     3061 aacgaacagg acaaccgctg gtatgccctc catgcaaagc tccctatgct cgaaccttcg
     3121 gacagccagg aatgtcttga tttcatgaag gccgcctttg atatctctga acgcttcgac
     3181 actccggttc tccttcgcgt cacgacgcgt atctgccaca gcaagagcgt cgtcgagaca
     3241 ggcgaacgta tggaacgact cattcgtgag tatacccgca atccggcgaa gaatgcgatg
     3301 cttcctgtct ttgcgaaggc gaagcactat ctcgtcgaaa aacgtcttct tgatctccgt
     3361 gagtttaacg agacgtcgcc gttcatctcg tacgtggagg gcgataagga aataggcgta
     3421 ctcacgagcg gcgtcggtta tcaacattcg agagaggcgc ttggcgacag ggtgagctat
     3481 ctgaaactgg gtatgacctg gcccctgcct gagaaagcca tccgggattt tgcttcgaaa
     3541 gtgaagaatc tttggattgt tgaggagaac gacgcattca tcgagggggc agtaaggggc
     3601 atgggcatcg cctgttccgg gaaggatgcg ctccctcttg tggatgaact ctctccggct
     3661 ctgatatcga aagcaattct cggaacaaag ccctcctccg gacccgatac cggaattgcg
     3721 ccgccgaacc gtcctccgct cctctgcgcc ggatgcccgc accgtgggct tctcttcgca
     3781 atgagcaaga agaaggatct ggttgtgatg ggagatatcg gctgctatgg acttgggtcg
     3841 atgcctccgc tcaacgtagg cgagagcacc atttgcatgg gagcggggtt ttcagcagtc
     3901 gtcggtttcc agaaagcgca ggaaaaggcc ggccgctccc agaaagtctt cggcatcctc
     3961 ggtgattcca cttttttcca ttccggtatt acgggcctta tcgacgctgt ggtgaacaag
     4021 tcgaggggtg tgttcgtcat ccttgacaac cggattaccg cgat
//
LOCUS       DAOPKU010000254         4033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24550_length_4033_cov_5.392660, whole genome shotgun sequence.
ACCESSION   DAOPKU010000254 DAOPKU010000000
VERSION     DAOPKU010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24550_length_4033_cov_5.392660"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PK849_08640"
     CDS             complement(<1..1131)
                     /locus_tag="PK849_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE66231.1"
                     /translation="MTFDVHEISPLEWDDMADGFLDLVDNEIRAAEMLLNADPPLAMP
                     SIPHSLIATEYLLFLAVQLLGASKQSWTNVDEYIKFIAEKAPDLRRTLGPLTPLKKAA
                     AAGAFDEDIFKVAESAFKATVKAEKILGEFIDKKLEELYAEHETSPASPSGGSGGRGN
                     EYGQAMEILDKAWGETSVKKRATLVKKALALCPDIADAYVILGNDALDSGKPEEAIEH
                     YRRGLEAGERELGAEAFTDDKGHFWGLLETRPYMRARFGLASALHKSGSVREASEHYK
                     AMLELNPNDNQGVRYMLIEALLLLGEHQDADALLKQYDEESAFMLYPKALLEFVKNGD
                     SKAAQKLRAEAIRNNPHVPAFLLGKKKLPKKTPDSYGFKDESE"
     gene            complement(1319..>4033)
                     /locus_tag="PK849_08645"
     CDS             complement(1319..>4033)
                     /locus_tag="PK849_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPE66232.1"
                     /translation="GKSAFNERLDALRKEWPEDALPPEEPDGLLAFATRLFDGILGNL
                     RGATTSQSGKLAQEALGCSFAEHRERFGTLWTGLRFLSQEPPEFDVRLRSVLLRTSAM
                     VWETYREFRRRRGLLSYDDMIRLAADIAASENSARSVRTFREILVDEYQDTNPLQDRL
                     IDSVAAEGCRRFLVGDPKQSIYRFRHADPTLFGERIASPSEETRYIPLQTSFRTRPAL
                     LKEINGLFGGIWRNGIASTLPTPYEELLFPENPEMRELREETSLPPLLPVFRRQRENE
                     PVAETRKRVALALGEKLLELHGQPVWNKKNKQMLPAEWRDMTILVPSRSSFAALEETL
                     HPLFGIPVAFEKGKRYFDRGEIGDLTTAIRMLAFPDDRAAALGFLASPFSGLNMEQFA
                     RFLGPEAPSLAEVFPETAARIEELRSVARYSGLFAALVLLLKDQSFLGAYPLWNRKSA
                     LANLWKGLDLVREYENVFGNDPAGCAAYVARMAGRKGAGEESTPLGEEEDVVRVLTVH
                     SAKGLEFPITVVMDLNNTPGGGGGRGALVPSALLGAGASRHPEAWSEKSASETGKIAR
                     FLEDVEVREEWERLFYVAATRAMDCLVLCSPCGMKEDAPSPVSGCSSESHDPEQRPLR
                     RTPREEERKQSVSIHPPVAAPRFVERMSATSYSLFRYCPAAWRMKFRQGIEMTWELPS
                     SEEAGGADLGSLAHWLLSRWDFRAASLPALIDEQHPRLPQGLRPAWRDERGRNALKGW
                     LERFAELPEGRKLACLHQTLSLRRELPFRVQIEGGAGSIRLVGSVDVLWVEGDTVYIR
                     DYKITAGEIDGAAAWEILYREQLLFYGYAASLAFPGRKQDIRLLHLREGREGERIKTP
                     ASWNEVGGSILSTASACATGPFPAAADRCPRCFFRLDCPYRIEISTGE"
BASE COUNT          677 a         1328 c         1268 g          760 t
ORIGIN      
        1 ctcgctctcg tctttgaatc cgtacgagtc gggcgttttc ttcggcagct tcttctttcc
       61 gaggaggaac gccgggacgt gcggattgtt ccggatcgcc tcggcgcgca gcttttgcgc
      121 cgccttcgaa tcgccgttct tcacaaattc caggagggcc ttcgggtaga gcatgaacgc
      181 gctctcctca tcgtactgct tcaggagcgc gtccgcgtcc tggtgctctc cgaggagcag
      241 cagcgcttcg atgagcatgt agcgcactcc ctggttgtcg ttcgggttca gttcgagcat
      301 cgccttgtag tgttccgagg cctcgcgtac gctgccgctt ttgtgcagcg ccgaggcaag
      361 accgaagcgg gcgcgcatgt aggggcgggt ttccagaagg ccccagaaat gccctttgtc
      421 gtcggtaaag gcttccgcgc ccagctccct ttccccggct tccagccccc tgcggtagtg
      481 ttcgatcgct tcttccggct tcccggaatc aagagcgtcg ttgcccagaa tgacatacgc
      541 gtcggcgata tctgggcaga gcgcgagcgc ctttttcacc agcgttgccc gcttcttcac
      601 cgacgtctcc ccccacgcct tgtcgaggat ttccatcgcc tgcccgtact cattcccgcg
      661 gccgccggaa ccgccggagg gagacgcggg cgaggtctca tgttcggcgt acagctcctc
      721 cagcttcttg tcgatgaatt cgccgaggat cttttcggcc ttcaccgtcg ccttgaaggc
      781 ggattcggcg accttgaaga tgtcctcgtc gaacgctccc gccgccgcgg ccttcttcag
      841 cggggtcagc gggccgagcg tacggcgaag atcgggcgcc ttctccgcga tgaacttgat
      901 atactcgtcg acattcgtcc aggattgctt cgaggcgccc aatagctgta cggccagaaa
      961 gagcaggtat tccgtcgcta tgagcgagtg aggtatcgag ggcatggcca gtggcggatc
     1021 agcgttcaac agcatctccg ccgcgcgtat ttcgttgtcg accaggtcga ggaatccgtc
     1081 ggccatatcg tcccattcaa gcgggcttat ctcgtgtaca tcaaacgtca tctcgatcca
     1141 tcccttcctt ccgtcttcga ctcccgaggg aggatgtgcg gttcggcgcg cgccccgaat
     1201 gccggatcgt cccggggaaa gtccggacgc acgcggcgaa cgaagcggtt tatcgttcac
     1261 acatccactc aatactaagc gtctctttct ctctcgtcaa tttctttttt gcagtttttt
     1321 attctcctgt agagatttcg atccggtacg ggcaatcgag acggaaaaaa cagcgggggc
     1381 aacggtccgc agccgctgga aacggccccg tcgcgcaggc ggaggcagtg gacagaatgg
     1441 agccgcccac ctcgttccac gatgccggag tcttgatccg ttctccttcg cgcccctcgc
     1501 gaagatggag cagcctgatg tcctgcttcc gcccggggaa ggccagcgaa gcggcgtagc
     1561 cgtagaagag aagctgctcc cggtagagaa tctcccaggc cgcggcgccg tcgatctctc
     1621 ccgcagtgat cttgtagtcc cttatataga ccgtatctcc ctcgacccag aggacgtcca
     1681 cggagccgac aaggcgaata cttccggcgc ccccctctat ctgtacgcgg aacggaagct
     1741 cgcgccggag cgagagcgtt tggtgcaggc aggccagttt ccggccctcc ggaagctccg
     1801 cgaagcgctc cagccaccct ttcagagcgt ttcttccccg ttcgtccctc catgcgggac
     1861 gaagcccctg cggcagacgc ggatgctgtt cgtcgatcag ggcgggaagg gaggcggcgc
     1921 ggaagtccca ccgcgaaagg agccagtgag ccagcgagcc gaggtcggct cccccggcct
     1981 cctcggagga gggaagctcc caggtcatct cgatcccctg acggaacttc atccgccacg
     2041 cggcggggca gtagcggaag agcgagtacg acgtggcgct catccgctcc acaaaccggg
     2101 gtgcagcaac cgggggatgg atggagacgc tctgcttccg ttcctcctcc cggggcgtcc
     2161 gccggagagg cctctgttcc ggatcgtggc tctcgctgct gcacccggaa acgggggagg
     2221 gggcgtcctc cttcatgccg cagggggagc agaggacgag acagtccatg gccctggtcg
     2281 ccgcgacgta aaagaggcgt tcccactcct ccctgacttc gacgtcctcc aaaaaacggg
     2341 cgatctttcc tgtctcggag gcgctcttct cgctccatgc ctcggggtgg cgcgaagcgc
     2401 ccgcgcccag cagcgcggag ggaacgagcg cgccccgtcc gccgccgcct cccggcgtgt
     2461 tgttcaggtc catcaccacc gtgatcggga attccagccc cttcgcgctg tggacggtga
     2521 gcacgcggac cacgtcttcc tcctcgccga ggggcgtgct ctcctcgccg gcccccttgc
     2581 gccccgccat gcgggcaacg taggcggcgc acccggccgg gtcgttgccg aagacgttct
     2641 cgtactcgcg gacaagatcg agccccttcc agaggttggc cagcgcgctc ttccggttcc
     2701 acagcgggta cgctcccagg aacgactggt ctttcagcag cagcaccagc gccgcgaaga
     2761 ggccggagta gcgcgcgacg ctccgcagct cctcgatcct ggccgccgtc tccggaaaga
     2821 cctccgcaag cgagggggcc tccggcccga ggaagcgggc gaactgctcc atgttcagcc
     2881 cggagaaggg ggaggccaga aaaccgagcg cggccgccct gtcgtcgggg aacgcgagca
     2941 tccggatggc agtcgtcaga tcgccgatct ctcccctgtc gaagtagcgc tttcccttct
     3001 cgaaggcgac ggggatgccg aaaagagggt gcagcgtctc ctcgagcgcg gcgaaggagg
     3061 agcgcgacgg cacgagaatg gtcatgtcgc gccactcagc agggagcatc tgtttgttct
     3121 ttttgttcca gacgggctgg ccgtgcagct ccaacagctt ctctccgaga gcaagcgcca
     3181 cccgttttcg agtctccgcg accggttcgt tttcccgctg acggcgaaag accggaagga
     3241 gcggagggag ggacgtctcc tcgcgcagct cccgcatctc gggattctcc gggaagagca
     3301 gctcctcgta gggggtggga agcgtggagg cgatgccgtt ccgccagatg ccgccgaaga
     3361 gtccgttgat ctccttcaac agcgcggggc gggtgcggaa gctcgtttgc agcgggatgt
     3421 agcgcgtctc ctcggacggc gaggcgatac gttctccgaa cagcgtcggg tcggcgtggc
     3481 ggaagcggta gatcgactgc ttcgggtcgc cgacaagaaa gcgcctgcac ccttcggcgg
     3541 cgacggagtc gatcagacgg tcctgcagcg ggttcgtatc ctgatactcg tccacgagga
     3601 tctcgcggaa cgtgcgcacg gagcgcgccg aattttcgga cgcggcgata tcagccgcca
     3661 gacggatcat gtcgtcgtac gacaacagcc ctcgtctgcg gcggaactcc ctgtaggtct
     3721 cccagaccat ggccgaggtc cgcagcagca cggagcgcag cctgacgtcg aactccggcg
     3781 gttcctgcga caggaagcgc agtcccgtcc agagcgtgcc gaagcgctcc cggtgctccg
     3841 caaaggagca gccgagcgcc tcctgcgcca acttcccgct ctggctcgtc gtcgcacccc
     3901 tgagattgcc gaggatgccg tcgaagagcc gcgtcgcgaa ggcgagcagt ccatccggct
     3961 cttccggcgg cagagcgtcc tcgggccact ccttccgcaa ggcgtcgagc cgttcgttga
     4021 acgcgctctt ccc
//
LOCUS       DAOPKU010000255         4032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24559_length_4032_cov_3.333920, whole genome shotgun sequence.
ACCESSION   DAOPKU010000255 DAOPKU010000000
VERSION     DAOPKU010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24559_length_4032_cov_3.333920"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..437)
                     /locus_tag="PK849_08650"
     CDS             complement(93..437)
                     /locus_tag="PK849_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPE66233.1"
                     /translation="MSLSRMFLFLTAIVVVFFLLPSVSNADEAPKKPTLVELMSPGCP
                     ACAEMEKVLAQFEEQYGDTVEVQIVNVREYPDVARLYQVRYVPTLVFIDENGKMLEKR
                     VGYMPLEALEKE"
     gene            582..1772
                     /locus_tag="PK849_08655"
     CDS             582..1772
                     /locus_tag="PK849_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPE66234.1"
                     /translation="MKKHEMTAIVEAMEEELIALRRRLHACPELDFALEGTVRIVEER
                     LSALRIPWRRVAGTGVAGLMKGKPGAGAVLLRADMDALPLREEVDVPWRSKNEGAMHA
                     CGHDVHTASLLGAAALLHKMGKQLPCDVLLVFQPAEETSGGALPMLSDGLLEGTDVRA
                     AFALHCDPSLPAGKVGVHGGAFRAASDMFDCVVRGRGTHGAEPHRGDDVIAIACRTVD
                     ALHQLVGRAVDPAEPAVISVGSFHAGTARNILPERAEFSGILRTIDPETRVSLRAKIR
                     RTVMNIPDVLGAVGEVRFTEGYPALINDPEMAGLVGGAVSDLLGWDALVRLTKPSMGV
                     DDFAYFAERAPGCYFMLGTGHGDGREEAPLHSPRFAPDERAIALGAALLAECALRAGE
                     GHQK"
     gene            complement(1760..2986)
                     /locus_tag="PK849_08660"
     CDS             complement(1760..2986)
                     /locus_tag="PK849_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE66235.1"
                     /translation="MITRSLIELVFSAASMERWNDHPRPAVFTELGKQAHKMIMAWVI
                     ARYESETRGVAVDWTALIEGGIFEFLHRVVLTDIKPPVFHKLMQNEEQRKKLNSWVAD
                     ALAFDLDRLSPDFAARFREFLLAEEDGSLERKSLRAAHYLATKWEFDFVYHWSNTKSM
                     FGIEQTRGEISRQINEHRDLRAVDEILAARDLPDRDMGLWGFLSLVGQLGFQKRWAQT
                     PRIPQTSVLGHLLFVAILSYFVSMEIGACPRRRYNNFFGGLLHDLPEVLTRDIISPVK
                     NSVAGLDELIKQLEKQAMEERILPLLPEAWREEIRYFTENEFAGKIRPHGSPSPVILQ
                     HDLGEEQNLDSLDPIDGRVIEACDKLSAYMEASLSIRLGVAPQALAEGKRNIYSRFGR
                     SVISGFPMGQLFDYFW"
     gene            3258..>4032
                     /locus_tag="PK849_08665"
     CDS             3258..>4032
                     /locus_tag="PK849_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66236.1"
                     /translation="MKGKFSSKVAVLAVAAVVLVAMFAVPALAKDTLTVANIYDARSL
                     DPIIQNEVAASNAICHINETLIELTDEGNPVPLLAETFEQLDSTTYKFVLRKGVKFHN
                     GDPFTAADVLYTIERAKSPLGSAIKQYPEIIESAEAPDDHTVIIKIKYPFTPFWGTIA
                     HTSLAIVNKRAVEEKGDMYPLEPVGTGPFQFKEWVKNDRLTLERFEDYWGTKPAYKTL
                     VIRSIPESVNRTIELETGAIDIAFRIPVSDVSRVKENKDL"
BASE COUNT          839 a         1107 c         1221 g          865 t
ORIGIN      
        1 agtcgtccca tgaaatatcg agtaaagaga ggaatagatt tcgcccatgg ataagcttat
       61 tcccctttac cgatattgta gccgcgtttt ctttattcct tttccagcgc ttcaagcggc
      121 atgtatccta ctcttttctc cagcattttt ccgttctcgt cgatgaagac cagagtaggt
      181 acgtagcgaa cctgatagag acgggcaaca tccgggtact cccgcacatt gactatctgg
      241 acctcgaccg tatcgccgta ctgctcctcg aattgggcca aaactttttc catctcagcg
      301 caggctgggc agcccggaga cataagctcc acaagcgtgg gctttttggg cgcttcgtcc
      361 gcgttggaaa cggacggaag caggaaaaat acgacaacta tagctgttaa aaaaagaaac
      421 atccttgaaa gactcatctc taatccctcc cgggtgtatt ttggtctgct aaacatactg
      481 gtattgtagt atttaaagtg gcggtaagtc aagtgggttt ttcaatccca tccccatttc
      541 tccggttttt cggtagaatg actgagaagg ggtgtgaaac tatgaaaaag catgaaatga
      601 ctgcgatcgt tgaagcgatg gaggaagagc tgatcgcgtt gcgtcgccgc ttgcatgcgt
      661 gtcccgagct ggattttgcg cttgaaggca cggttaggat cgtggaagaa cggctttccg
      721 ctctaagaat cccatggagg cgcgttgccg gtaccggcgt tgcggggttg atgaaaggca
      781 agccgggcgc cggcgccgtg ctgttgcggg cggatatgga cgcgctgcct ctgcgggagg
      841 aagtggacgt cccgtggcgt tcgaaaaacg agggcgccat gcacgcgtgc gggcacgatg
      901 tgcacacggc gagtcttttg ggcgccgctg cactgcttca caagatgggg aaacagcttc
      961 cctgcgatgt gctgctggtt tttcagcctg ccgaggagac gtccgggggc gcgctgccga
     1021 tgctttccga cggccttctt gaggggacgg acgtcagggc ggcgtttgcg ctgcactgcg
     1081 acccgtcgct tcccgcagga aaggtcgggg tgcacggagg cgctttccgc gcagcctccg
     1141 acatgttcga ctgcgtggta cgcggcaggg gaacgcacgg cgcggaacct caccgggggg
     1201 atgatgttat cgcgatcgcg tgcaggacgg tcgacgcgct gcatcagctc gtcggcaggg
     1261 ccgtcgaccc cgccgagccc gccgtgatct ccgtcggttc gtttcatgcg gggacggcgc
     1321 ggaatatcct tcccgagcgg gcggagttct ctgggatcct gcggacgatt gatcccgaga
     1381 cgcgggtgag tttacgggcg aagatccgca gaacggtgat gaatattccg gacgttttgg
     1441 gggcggtggg ggaggtgcgc ttcaccgaag ggtatccggc gctcattaac gatcctgaga
     1501 tggcaggctt ggtggggggc gcggtttcgg acctcctggg ttgggacgcc ctcgttcgtc
     1561 tgacgaagcc gagcatggga gtcgacgact tcgcctattt cgctgagcgg gcgcccggct
     1621 gctattttat gctgggcacg gggcacggcg acggacgtga agaagcgccg ctccacagcc
     1681 cccgcttcgc ccccgatgaa agggcgattg ctctcggggc cgcccttctc gccgagtgcg
     1741 ccctccgcgc cggggaaggt caccagaagt agtcgaagag ctgccccatc gggaaaccgg
     1801 agatcaccga gcggccgaag cgggagtaga tgttccgctt cccctccgcg agcgcctgcg
     1861 gagcgacgcc caggcggatc gagagcgagg cctccatata ggcggagagc ttgtcgcacg
     1921 cctcgatgac gcgaccgtcg ataggatcga ggctgtcgag gttctgctcc tctccgaggt
     1981 cgtgctgcag aatcaccggc gacggcgagc cgtgtggacg tatcttcccg gcgaactcgt
     2041 tttcggtgaa ataacgtatc tcttcgcgcc acgcttccgg gaggagcgga agaatccgct
     2101 cttccatagc ctgtttctcc agctgcttga tgagctcgtc gagtccggct acggagttct
     2161 tcacaggaga aatgatgtcg cgcgtgagta cctccggaag gtcgtgcaga aggccgccga
     2221 agaagttgtt gtaccgcctt ctggggcacg cgccgatctc catggagacg aagtaggaga
     2281 ggatcgcgac gaaaagaagg tgtccgagca ccgacgtctg cggaatcctt ggggtttgcg
     2341 cccatcgctt ctgaaagccc agctgcccca ccagcgagag gaagccccag agccccatgt
     2401 cgcggtcggg cagatcgcgc gcggcaagga tctcgtccac cgccctgagg tcgcggtgtt
     2461 cgttgatctg ccgggagatc tcccccctcg tctgctctat gccgaacatc gacttcgtgt
     2521 tgctccagtg gtagacgaag tcgaactccc acttggtcgc cagatagtgg gcggctcgca
     2581 agcttttccg ttcgagcgag ccgtcctcct cggcgaggag aaactctctg aatcgcgccg
     2641 cgaagtcggg ggagaggcgg tcgaggtcga acgcgagggc gtccgcgacc catgagttca
     2701 gttttttccg ctgctcctcg ttctgcatca gcttgtggaa gacggggggt ttgatgtcgg
     2761 tgagcacgac cctgtggagg aattcgaaga tgccgccctc gatgagggcg gtccagtcga
     2821 ccgcgacgcc gcgcgtttcc gattcatagc gggcgatgac ccacgccatg atcatcttgt
     2881 gcgcctgttt gccgagctcc gtgaagactg ccggccgcgg atgatcgttc cagcgttcca
     2941 tactggcggc ggagaagacg agttcgatga gcgagcgtgt gatcattaga agggcctccc
     3001 gaaaagtaat tcaaatatct tctccgcaga gagtgcaatc gtgcggaggg tttgaataaa
     3061 atgattatac aacctttggc caagattgct tcgaaaaatc gggtgcgatt atgtatgcat
     3121 aatacttctt gacaatatgt cggtccgtgc ataataattc aagggcatca ctgaagaaga
     3181 aactcttggg gaaaataaat aacaaaagtt tcttttttct tctgatgcaa aaaaaaatcc
     3241 acaaaggaga tgaaagtatg aaaggaaagt tcagttccaa ggttgcggtt cttgccgtag
     3301 ccgcagtggt tcttgtggcc atgttcgcgg ttccggcgct ggcgaaggat acgctcaccg
     3361 tcgccaacat ttacgacgcg cgctctctcg acccgattat ccagaacgag gtggctgcaa
     3421 gcaacgcgat atgccacatc aacgagacgc ttatcgagct cactgatgag ggcaatcccg
     3481 ttcctctgct tgcggagaca ttcgagcagc ttgattcgac gacctacaag ttcgttctgc
     3541 gcaagggcgt gaagttccac aacggcgatc cctttactgc tgctgacgtc ctctacacca
     3601 ttgagcgggc gaagagccct ctcggctcgg cgatcaaaca gtatcccgaa atcatcgaga
     3661 gcgctgaagc tccggacgac cataccgtca tcatcaagat caagtatccg ttcactccct
     3721 tctggggaac catagcccat acctccctcg cgatcgtgaa caaacgcgcc gttgaggaga
     3781 agggcgacat gtacccgctc gagccggtag gcaccggtcc cttccagttc aaagagtggg
     3841 tgaagaacga ccgtttgact cttgagcgct tcgaagatta ttggggaacg aaacccgcgt
     3901 ataagaccct cgtaatccgt tccattcccg agagcgtcaa ccgcaccatt gagcttgaga
     3961 caggcgcaat cgacattgcc ttcagaattc cggtttccga cgtctcccgc gtgaaagaga
     4021 acaaggacct cg
//
LOCUS       DAOPKU010000256         4027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24595_length_4027_cov_5.040282, whole genome shotgun sequence.
ACCESSION   DAOPKU010000256 DAOPKU010000000
VERSION     DAOPKU010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24595_length_4027_cov_5.040282"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1138)
                     /locus_tag="PK849_08670"
     CDS             complement(20..1138)
                     /locus_tag="PK849_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HPE66237.1"
                     /translation="MFRKAPLTSLCLLFCLFALPASAAEMTVTDVLGRSVTVPASPER
                     IIASGSGALRLIVYLQAQDRVVAVDSAERRVPNLAVLVSARPYSIANPQFQDMPVFGE
                     FRGMDNPELIAGLSPQPQVIFKVSPLSGPHPDQLTAKTGIPVVGLEYGNLTDKKSDFY
                     ATLRTMGQILGKSERAEEVVAFFERHLAELSRRTADIPDDKRPACYIGGVASRGAHGF
                     TSTEPGYPPFLYTNAKNVAALPGEKTSAVAQISKEKLLEWDPEILFVDLSTTTAGEQA
                     SSLYQLTHDPALSALSAVREGNIWGVLPYNSYTLNYGSVLANGYFVGKTLYPDRFKDI
                     DPAATADEIFTFLVGQPVFSRMNEEFSGRAFSKIALGE"
     gene            1307..1870
                     /locus_tag="PK849_08675"
     CDS             1307..1870
                     /locus_tag="PK849_08675"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPE66238.1"
                     /translation="MESLEKIVAENLKRLRGERKLSLDRVSALTGVSKSMLGQVERGE
                     SSPTLQTVWKIANGLRIPLSELTDAPKQETESFSKEQVAAILGDNGKFRVYPIFPFDG
                     KNRFEFLSIEMDRGAFSSSEPHIAGTVEYVHVFEGEVVIMTGSEERRLECGDSMKYRA
                     DRPHSYHNPGGERAVLAMVILYPEENV"
     gene            1886..>4027
                     /locus_tag="PK849_08680"
     CDS             1886..>4027
                     /locus_tag="PK849_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPE66239.1"
                     /translation="MSSSERTESARQEYVHGTPSVSCLRAAIWTESHKETEGEATPVR
                     RAKAFAAACGKLPAIIFPGELIVGVSGEFRRSAILTPEFSWTWVDREMDSFDTRPQDP
                     YRMSPQQREFARSAIWPYWKGKSLEEAFLKRLPEDTARLLVDTGILDNDSKWRQAVGE
                     VTPDYQDVLFPKGYRRIRDEAAAHLAATKPDSLENLERRDFYHSVVIACDGIMRLAER
                     YSEEAMRLAEKEADPVRRGELLEIAGNCARVPAEPPRTFAEACQFVWFVQLGAILSEN
                     PLALNPGRFDQYMYPYYAADVEAGRLTPERALELVECLWIKFSEWVWTISSNTANYFA
                     GYNQFQNLTVGGRKRDGSDGTNELSYICLKATEGVKTHQPGLSVRISSDCPDDFLMAV
                     SKLVATGMGFPAIHNDQAGAQMLLQAGYEPEDARDWNNCGCVVPHFRKTGEWTSAVNV
                     NFGAAMEYALNEGKSRLTGEPMGLPERAPEEFVSFREVADAFLRQLANLVEHAVVGTL
                     TAQKLHMEMVPRPFLSTCVDGCMQKGKDLSRGGAVYNVGPVLTGIGLGVVANSLAAVK
                     KLVFDEKVCTMKELCAALNADWEGYDELRERALAAPKYGNDDDEVDSLAREISDFYYR
                     TTRSHRDVFGSPFNSAFMGISNYVPTGKVIGALPCGRKARAPLSEGVSPFAGTDVSTP
                     LAAMRSAAKVNHDVQTGGTLLNLRLNKEIVST"
BASE COUNT          775 a         1192 c         1368 g          692 t
ORIGIN      
        1 tccttccaga tgcacgtcgc tactccccga gtgcgatttt cgagaacgcg cgccccgaga
       61 actcctcgtt catcctcgaa aagaccggct gtcccacgag aaaggtgaag atctcgtcgg
      121 ccgtcgccgc cgggtcgatg tccttgaagc ggtcggggta gagcgtcttc ccgacgaaat
      181 agccgttggc gagcaccgag ccgtagttca gcgtgtacga gttgtagggc agcacgcccc
      241 agatgtttcc ctcgcgcacg gcggaaagcg ccgagagcgc ggggtcgtgc gtcaactggt
      301 agagcgagct tgcctgttcg cctgcggtcg tggtgctcaa gtcgacgaag agaatctccg
      361 gatcccattc gagcagcttc tccttggaaa tctgagcaac ggcggaggtc ttctcgccgg
      421 ggagcgcggc gacgttcttc gcgttcgtat agaggaaggg cgggtatccc ggttccgtcg
      481 aggtgaagcc gtgcgcgccg cgcgaggcca cgcccccgat gtagcacgcg ggacgtttgt
      541 cgtcgggaat gtccgcagtc cggcgggaga gttcggcgag gtggcgctcg aagaaggcca
      601 cgacctcctc cgcccgttcg ctcttgccga ggatctgtcc catggtgcgg agcgtggcgt
      661 agaagtcgct cttcttgtcg gtgagattgc cgtactcaag ccccaccacc ggaatgccgg
      721 ttttggccgt gagctggtcg gggtgcgggc cggagagcgg ggagaccttg aagatcacct
      781 gcggctgcgg cgagagcccg gcgatcagct ccggattgtc cattccgcgg aactcgccga
      841 agaccggcat gtcctggaac tgcgggttcg cgatggagta cgggcgggcc gagacgagga
      901 cggcgaggtt gggaacgcgc cgttcggcgg agtcgacggc gaccaccctg tcctgcgcct
      961 gaaggtagac gatcaagcgg agcgctccgg aaccggaggc gatgattcgc tcgggcgagg
     1021 cgggtactgt cacggagcgg ccgaggacgt cggtcacggt catctccgcg gcggacgcgg
     1081 gtagggcaaa caggcagaaa agaagacaga gagaggtcaa gggtgctttg cgaaacacgg
     1141 aaatccactc cttataaaaa acatgttcag tgcgcaggta atagtagcac gaaaacgttt
     1201 atttgtgtta tcaagttgca cccttcgtgg gaaatctctt gacagaaacg ttctttttgt
     1261 gcaatataac gcacataaag aacgctgtgt cagggaggca agagctgtgg aaagtctcga
     1321 aaagatcgtc gcggaaaatt tgaagcgtct gcggggggag cggaagctca gccttgacag
     1381 ggtgtcggcc cttaccggcg tgagcaagag catgctgggg caggtcgagc ggggagagtc
     1441 gagcccgacg ctccagacgg tctggaagat cgccaacggc ctgcgcatcc ccctcagcga
     1501 actcaccgac gctcccaaac aggagacgga gtccttctcc aaggagcagg tggcggcgat
     1561 actgggagac aacggaaagt tcagggtgta ccccattttc cccttcgacg ggaaaaaccg
     1621 cttcgaattc ctctccatag agatggacag gggggcattt tcctcctccg aaccgcatat
     1681 cgccgggact gtggagtacg ttcacgtctt cgaaggcgag gtcgtcatca tgacggggag
     1741 cgaggaacgg cggttggaat gcggggactc gatgaagtac agagcggaca ggccgcactc
     1801 ctatcacaac ccgggagggg agcgcgcggt gcttgcgatg gtcatcctct atccggagga
     1861 gaatgtctga aaggggagtt gtttcatgag cagcagtgaa cgcaccgaga gtgcgcggca
     1921 ggagtacgtg cacggcacgc cgtccgtgag ctgccttcgg gcggcgatat ggacggagtc
     1981 ccacaaggag accgaagggg aggcgacgcc ggttcggcgc gcgaaggcct tcgctgcggc
     2041 gtgcgggaag ctcccggcga tcatctttcc cggcgagctg atcgtcggcg tctccggcga
     2101 gtttcgccgc tccgccatcc tcacgccgga gttctcgtgg acatgggtgg accgcgagat
     2161 ggactccttc gacacgcgcc cgcaggaccc gtacaggatg tccccgcagc agcgggagtt
     2221 cgcccggtcg gcgatctggc cgtactggaa ggggaagtcc ctcgaagagg cgttcctgaa
     2281 gcgtctgccg gaggatacgg ctcgtctgct cgtggacacg ggaatactgg ataacgactc
     2341 gaagtggcgg caggcggtgg gcgaggtgac gcccgactat caggacgtcc tctttccgaa
     2401 agggtaccgg cgaatccgcg acgaggcggc ggcgcatctg gccgcgacga agcccgattc
     2461 gctcgagaac ctggaaagac gcgacttcta ccactccgtc gtcatcgcct gcgacggaat
     2521 catgcgtctt gcggagcgct actccgagga ggccatgagg ctcgccgaga aggaagcgga
     2581 cccagtacgg cgcggcgagc tgctggagat cgccgggaac tgcgcccgcg ttcctgcgga
     2641 acctccccgg acgtttgccg aggcctgcca gttcgtctgg ttcgtccagc tcggcgcgat
     2701 cctctccgaa aacccgctgg ccctcaatcc gggccggttc gaccagtaca tgtacccgta
     2761 ctacgcggcc gacgtcgagg ccggacgcct gacgccggag cgggcgctcg aactggtgga
     2821 gtgcctctgg atcaagttct cggagtgggt ctggacgatc tcctccaaca cggcgaacta
     2881 cttcgccggg tacaaccagt ttcagaatct caccgtcggc gggcggaagc gggacggaag
     2941 cgacgggacg aacgagctct cctacatctg tctgaaggcc acggagggag tgaagacgca
     3001 ccagcccggg ttgagcgttc gtatttcttc ggattgcccg gacgacttcc tgatggcggt
     3061 ctcgaagctc gtcgccacgg gaatgggttt ccccgcgatc cacaacgacc aggccggggc
     3121 gcagatgctg ctccaggccg ggtacgagcc ggaggacgcc cgcgactgga acaactgcgg
     3181 ctgcgtggtc ccgcacttcc gtaagacggg ggagtggacc tcggcggtga acgtgaactt
     3241 cggcgcggca atggagtacg ccctcaacga aggaaaaagc cgcctcaccg gagagccgat
     3301 ggggcttccc gagagagctc ccgaggagtt cgtctccttc cgcgaggtcg cggacgcctt
     3361 cctccggcag cttgccaacc ttgtggagca cgccgtcgtg ggcaccctga cggcgcagaa
     3421 gctgcatatg gagatggtcc ccaggccctt cctctcgacc tgcgtggacg gctgtatgca
     3481 gaaggggaaa gacctctcga gggggggcgc ggtgtacaac gtcggccccg tcctcaccgg
     3541 tatcggcctc ggcgtggtcg ccaactccct cgccgcggtg aagaagctgg tcttcgacga
     3601 gaaggtctgc accatgaagg agctgtgcgc cgcgctcaac gccgactggg aggggtacga
     3661 cgaactgcgg gagcgcgctc ttgcggcgcc gaagtacggc aacgacgacg acgaggtcga
     3721 ttcgctggcg cgggagatct ccgacttcta ctacaggacg accagaagcc acagggatgt
     3781 gttcggctcg cccttcaatt ccgccttcat gggaatctcc aactacgtcc ccaccgggaa
     3841 agtgatcggc gcgcttccct gcgggaggaa ggcgcgggcg ccgctctccg agggggtctc
     3901 cccgttcgcg gggacggacg tctccacacc gctggcggcc atgcgttccg cggccaaggt
     3961 gaaccacgac gttcagaccg gcgggacgct gctcaacctg cggctgaaca aggagatcgt
     4021 ctccacg
//
LOCUS       DAOPKU010000257         4011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24731_length_4011_cov_5.112993, whole genome shotgun sequence.
ACCESSION   DAOPKU010000257 DAOPKU010000000
VERSION     DAOPKU010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24731_length_4011_cov_5.112993"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..735
                     /gene="lepB"
                     /locus_tag="PK849_08685"
     CDS             196..735
                     /gene="lepB"
                     /locus_tag="PK849_08685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPE66240.1"
                     /translation="MAAAKPWWRETIETILWALVLALILRTFVVQAFWIPSGSMIPTL
                     EVGDRVLVAKFWYHFQEPKRGQLFVFKYPVDPKRDFVKRIIGLPGDMLEVRNGAVLIN
                     GQPLQEEYVKNHDSFSLTAGPVFPEVPVKIPANSYFAMGDNRPNSQDSRFWGFVPKQN
                     IRGPVFFRYWPLNRIGMVD"
     gene            736..1557
                     /locus_tag="PK849_08690"
     CDS             736..1557
                     /locus_tag="PK849_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPE66241.1"
                     /translation="MGRTVWYPGHMAKGKRKLSELAGKLDIIIEVRDARAPAQTSSPL
                     VGELSSVCPIAVALSKRDLADEKATAKWLSYFAASGQKAWALDLLKPRMDQVRRDLAI
                     FGPAHREVRLAVVGIPNVGKSMFLNALVGKSSAQVGGIPGITRGVSWYKGKGFLAVDS
                     PGILDPKADDAVQRCLAWLGSSKAEVIGGYENVALDLIAVLRRKNLWGMVEAKWNVAA
                     PAETSDEDVLEALGRRLGCLVSGGAVDLSNAARRFVEAFSTGRLGRVTLELPEES"
     gene            1557..2144
                     /locus_tag="PK849_08695"
     CDS             1557..2144
                     /locus_tag="PK849_08695"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPE66242.1"
                     /translation="MELKEFGRIAGTDEAGRGPLCGPVVAAAVILTETQRDELLALGL
                     ADSKKLSPRKREALFDRMLSLGVLWRAQAASHERIDRMNILRASLWAMRRSLMKLPPL
                     FDTVFVDGTFTLPDLPFRQLALPKADSLVPAVAAASVVAKVLRDRVMDALDALYPGYG
                     LKKHKGYPTAAHRAALLSLGPSPVHRKSFSWRTPS"
     gene            2141..3193
                     /locus_tag="PK849_08700"
     CDS             2141..3193
                     /locus_tag="PK849_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66243.1"
                     /translation="MTPPNIGGITGPQIRPGLPDAQQPRVDGAPQQQGKSLSTIPNGT
                     VVNGTVLEAREAGAYLVRVAGQTLTAQANLPLLVGQHFRAVWDSSGDIPVLRLSDAEQ
                     ALLGKLSSGDKEVASALIARGLSLDPKALASIRAAWNGMGQQPGQLAPLAELWARGLP
                     MTSANVQVLSWYLSLDEQKTSSLWKRTRDELRERLARGDDPRKALRSLLGKDDDIAAF
                     LRGHGLLSSPSREGVDPSLLAGAFLPAGDDAQPLNARIATSAFKRGEKSFWSVSFEME
                     GDRLGLVAGDVETDGRSLSVVLKAEQEASFEELRRRRHILRHELEEIPLSLQNVAVAH
                     GRRMPRVPGRGLDITV"
     gene            3190..3468
                     /locus_tag="PK849_08705"
     CDS             3190..3468
                     /locus_tag="PK849_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HPE66244.1"
                     /translation="MSPPKKKRAQAAAVQYDQKNDDAPRIVASGEGYVAEKIVELARQ
                     ADVPVVEDAALVSALLVLELGEEIPGELYEAVAKILTFVYKLDKGEKL"
     gene            3465..3839
                     /locus_tag="PK849_08710"
     CDS             3465..3839
                     /locus_tag="PK849_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPE66245.1"
                     /translation="MTAEHLLTGRRGEELAEAHLRAVGWTILERNVTFRGGELDIVAR
                     DGNELVVVEVRTRAPGWMQSGEESVGPRKIRRLVRTGMKYMDSRKWDGPWRIDVVAVT
                     LPENGDAYIERFEDITAGGNFA"
     gene            3836..>4011
                     /locus_tag="PK849_08715"
     CDS             3836..>4011
                     /locus_tag="PK849_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPE66246.1"
                     /translation="MNGVQGITLRGIEAVAVEVEAEITGGLFSIAIVGLPDAAVRESK
                     ERVRAALRFLGMAV"
BASE COUNT          744 a         1226 c         1287 g          754 t
ORIGIN      
        1 ggtatatcgt cgtctctccg tacggacggg tttcgctgcg tgatacgccg ccgtaatgcg
       61 gaaagcggcg tttttatgtg aaaatgttac tgcgggattg aaactcttgc tttttcgggg
      121 tataaaagag gccgatactg aaaatatgaa ccctctttcc ccatcggttc ttcactttga
      181 aacggaggtc attgtatggc agcggcgaaa ccctggtggc gcgagaccat cgaaacaatt
      241 ctctgggctc ttgttctggc cctcattctc cggacgttcg ttgtgcaggc gttctggatt
      301 cccagcggtt ccatgattcc caccctcgag gtcggggaca gggtgctggt ggccaagttc
      361 tggtaccact tccaggagcc gaagagagga cagctcttcg tcttcaagta ccccgtggac
      421 cccaagcggg atttcgtcaa acgaatcatc gggcttccgg gcgatatgct cgaagtgcgc
      481 aacggcgccg tcctcatcaa cggacagcct cttcaggaag agtacgtcaa gaatcacgac
      541 agcttctcgc tcaccgcggg tccggtcttt ccggaggtcc cggtcaagat accggcgaac
      601 tcctacttcg ccatgggcga caacaggccg aactcgcagg acagccgttt ctgggggttt
      661 gtcccgaagc agaacatccg cggcccagtc ttcttccgct actggcctct gaacaggatc
      721 gggatggtgg actagatggg gcgtaccgtc tggtatcccg gccatatggc caagggaaag
      781 cgaaagcttt ccgaactcgc cgggaaactg gacatcatca tcgaggtgcg cgacgcgcgg
      841 gctccggcgc agacatcctc tccgctcgtc ggagagctct cttccgtgtg ccccatagcc
      901 gtcgcccttt cgaagcgcga cctcgcggat gaaaaggcca ccgcaaagtg gctctcttac
      961 tttgccgcat cggggcagaa ggcgtgggcg ctcgatctgc tcaagccaag aatggaccag
     1021 gtcaggcgcg atctcgccat tttcggaccg gcgcaccgcg aggtacggct ggcggttgtg
     1081 ggcattccga acgtcggcaa atcgatgttt ctcaacgcgc ttgtaggaaa atcctccgcg
     1141 caggtggggg gcattcccgg aattacgcgc ggcgtttcat ggtacaaagg gaagggattc
     1201 ctcgcggtgg attcgcccgg aatcctcgac ccgaaagcgg acgacgccgt gcagcgctgc
     1261 cttgcatggc ttggaagcag caaggcggaa gtcatcggag ggtatgagaa cgtcgctctt
     1321 gacctcatcg cggtccttcg ccgaaagaac ctgtggggga tggtcgaggc caaatggaac
     1381 gtcgccgcac ccgctgaaac gtccgacgaa gatgttctcg aggcgctcgg gagacgtctt
     1441 ggctgtcttg tttccggcgg tgcggtggat ctatcgaacg ccgcacggcg ttttgtcgag
     1501 gccttttcga ccgggcgtct tggccgcgtg acgctggagc ttccggagga gagctgatgg
     1561 aactgaagga gttcggacgt atcgccggaa ccgacgaggc ggggagagga cctctctgcg
     1621 gaccggtcgt cgccgccgcg gtcatcctga cggaaacgca gcgcgacgaa ctgctcgcgc
     1681 tcggcctcgc cgactcgaag aagctgtcgc cgcggaaacg cgaggcgctc ttcgacagga
     1741 tgctttctct cggcgtactc tggcgcgcgc aggcagcctc tcatgaacgg atcgaccgca
     1801 tgaacatcct gcgcgcctcc ctgtgggcga tgcggcggag cctgatgaag cttcccccgc
     1861 tcttcgacac ggtcttcgtc gacggcacgt tcactctccc cgatcttccg ttccgccagc
     1921 tggcgcttcc gaaggccgat tccctcgttc cggccgtcgc cgcagcctcc gtcgtggcca
     1981 aggtgcttcg cgaccgcgtg atggatgctc tcgacgcact ctaccccgga tacggtttga
     2041 agaaacacaa ggggtaccct acggcggcgc acagagctgc gctgctttcc ctgggtccct
     2101 cccccgtgca tagaaaaagc ttttcgtgga ggactccctc atgacccccc cgaacatcgg
     2161 cggcatcacc ggcccgcaaa tacgccccgg actccccgac gcccagcagc cgagggtcga
     2221 cggcgctccg caacagcaag gcaagtctct ctccacgatc ccgaacggaa ctgtggtgaa
     2281 cggaaccgtc cttgaagcgc gtgaagcagg cgcctacctc gtgcgggtgg cggggcagac
     2341 gctcacggcg caggccaacc tgccgcttct ggtcgggcag cactttcggg ccgtctggga
     2401 ctcttccggc gacatccctg tgttgcgcct ctccgacgcg gagcaggcgc tccttggcaa
     2461 gctctcctcg ggagacaaag aggtcgcctc ggccctcatc gcccgggggc tttcgctgga
     2521 cccgaaggcg ctcgcctcga tccgtgccgc gtggaacggc atggggcagc agccgggtca
     2581 gcttgctcct ctggcggagc tctgggcccg cggtctcccg atgaccagcg ccaacgtcca
     2641 ggtgctttca tggtacctct ctctcgatga acaaaaaaca tcctccctct ggaagaggac
     2701 acgggacgaa ctgcgcgaac gcctcgcccg cggcgacgat ccgcggaaag ccctgcgatc
     2761 gctgctcggt aaggacgacg atatcgccgc ctttctgcgc gggcacggtc ttctttccag
     2821 cccgtcgcgc gaaggggtgg atccgtcgct gctcgcgggg gcattcctcc ccgccggaga
     2881 cgacgcgcag ccgctgaacg cccggattgc cacaagcgcc tttaaaagag gagaaaaatc
     2941 gttctggtcc gtctcctttg aaatggaggg ggatcggctc gggcttgtgg cgggagatgt
     3001 ggagacggat ggacgatcct tgagcgtggt gctgaaggcg gaacaagagg cgagcttcga
     3061 ggagcttcgc cgtcgccgcc atattcttcg tcatgaactc gaagagattc cgctttccct
     3121 gcagaacgtc gccgtggccc acggaaggcg aatgcctcgc gtgccggggc gtggactgga
     3181 catcacggta tgagcccccc gaaaaaaaag cgcgcccagg cggccgcagt ccagtacgat
     3241 cagaagaacg acgatgctcc gcgcattgtc gcctctggag aggggtacgt cgcggagaag
     3301 atcgtggagc tcgcgcgcca ggccgacgtt cccgtcgtcg aggacgccgc gcttgtgagc
     3361 gcgctgctcg tccttgaact gggcgaggaa atcccgggag agctgtacga ggccgtcgcc
     3421 aagatactca ccttcgtcta caaactcgat aagggagaaa aactatgacg gcggagcacc
     3481 ttctgacggg ccgccgcggc gaggagcttg ccgaagcgca tctgcgcgcc gtcggatgga
     3541 cgattcttga acgaaacgtc accttcaggg gaggcgagtt ggatatcgtc gcccgcgacg
     3601 ggaacgagct cgtcgtcgtg gaggtgcgca cgcgcgcgcc cggttggatg cagagcggcg
     3661 aggagtcggt ggggccgaga aagatccgcc gccttgtccg caccgggatg aaatacatgg
     3721 attcccgcaa atgggacggt ccgtggcgga tcgacgtggt ggccgtgacg ctgccggaga
     3781 acggagatgc gtatatcgag cggtttgagg acatcacggc gggaggtaat ttcgcatgaa
     3841 cggcgtccag gggatcacgc tccgcggtat cgaagcggtc gccgtcgagg ttgaagccga
     3901 gatcacgggc gggcttttct ccatcgccat cgtcggcctt cccgacgcgg cggtgcgcga
     3961 gtcgaaagaa cgggtccgcg cggcgctccg ctttctggga atggccgtca g
//
LOCUS       DAOPKU010000258         3999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24827_length_3999_cov_4.094320, whole genome shotgun sequence.
ACCESSION   DAOPKU010000258 DAOPKU010000000
VERSION     DAOPKU010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24827_length_3999_cov_4.094320"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1680)
                     /gene="ilvD"
                     /locus_tag="PK849_08720"
     CDS             complement(4..1680)
                     /gene="ilvD"
                     /locus_tag="PK849_08720"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPE66247.1"
                     /translation="MKLHSANATKDANHRNLFKSMGYSDSDLSGPIIGIANSWNELVP
                     GHFNLRQIAEYVRKGVHKAGGAVAEFGVIAGCDGISEGHEGMKYILPSRELIANDVEM
                     MVRAHSLDGIVLLGSCDKIVPGMLMAAARLGIPAILLPGGPMLGGVEFDGRKSDSTTN
                     AEAFGMLQRGLIDEKTYRNLEGLCSPTCGSCSFLGTANTMCCLSEALGMTIPDGALVP
                     AVYAERLRLAEQTGKAIVALVEKGIGAHDIITRDSLENAVRVLMATGGSTNAVLHLSA
                     IAAEIGIPAEEMMALYERCSRTTPHVAKVNPASKHDMEAFHLAGGIPRVMKEIRALLH
                     TDCVTVTGSAVGENLDAHVERYPANPEIIRPASDPFGVNLGLAVLRGNLAPDTAITKP
                     AAIDPSMRVFSGAARVFDGEDAADEAILAGEIPEGTVVVIRYEGPKGGPGMREMFAAM
                     KYLYGMGLAKKTALVTDGRFSGTNNGCFVGHVSPEAAEGGPLAIVRDGDRITIDIPAG
                     SLRIDLTDEEIAERLRAWKRPEPTVKRGFLATYARLAGSAAEGAVMKLPE"
     gene            complement(1734..2213)
                     /locus_tag="PK849_08725"
     CDS             complement(1734..2213)
                     /locus_tag="PK849_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPE66248.1"
                     /translation="MSKSIGLLFWLGIVFAAAAVGGLASVESASFYKELARPSWAPPG
                     WLFGPVWTALYAMMGVAAWLVWRERGFRGGLVPLSLFIDQLVLNALWTWIFFVWRQGM
                     WAFVEILVLWALIVATMKAFHAVRPLAAWLLAPYLAWTTFAAALAFTVWRMNPALLG"
     gene            2594..2920
                     /locus_tag="PK849_08730"
     CDS             2594..2920
                     /locus_tag="PK849_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66249.1"
                     /translation="MEAATPYETILYLARKYIWWKTPEEAAAMPERVLAQVMNIGDYD
                     DVLLMKSRFGDDALRNVIAHAEAGQFNARSWHYWHYMLGLAEIDHVPPLPTRRFSWRG
                     AGKTTS"
     gene            complement(2910..3197)
                     /locus_tag="PK849_08735"
     CDS             complement(2910..3197)
                     /locus_tag="PK849_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HPE66250.1"
                     /translation="MTLRYEIQRTSRFKRDFALVERRGCDISLLADVIRALASGETLP
                     RQYRDHTLSGDYAEYRECHIRPDWLLIYKITEESLVLTLERTGTHSDLFKK"
     gene            complement(3194..3424)
                     /locus_tag="PK849_08740"
     CDS             complement(3194..3424)
                     /locus_tag="PK849_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66251.1"
                     /translation="MPRTTLEISAKKELYRRFRKLSERNAMRVLGYIDALEEERPNEE
                     TVAALEEGERMLKDPDALRFSTVEDLLTELRS"
     gene            3692..>3999
                     /locus_tag="PK849_08745"
     CDS             3692..>3999
                     /locus_tag="PK849_08745"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPE66252.1"
                     /translation="MTEFYAVIDRDEDGQFFAYVPELPGCHTCGETMEELLENLEEAI
                     ALYLEVVEHPREFPSIRKVAVGEPPQMLFPISRAFSSAPVDTTSPLLLIPRTPSEPRS
                     "
BASE COUNT          731 a         1270 c         1234 g          764 t
ORIGIN      
        1 ggttcactcc ggcagcttca tgacggcgcc ctcggcggcg gagccggcca gtctggcgta
       61 ggtggccaga aagccgcgct tcaccgtcgg ttcggggcgc ttccaggcgc gcaggcgctc
      121 ggcgatctcc tcgtcggtca ggtcgatgcg cagcgagccc gcggggatgt cgatggtgat
      181 ccggtcgccg tccctgacga tcgcgagcgg acctccctcg gccgcctcgg gggagacgtg
      241 cccgacgaag cagccgttgt tggtgccgga gaatcgcccg tcggtgacga gcgcggtctt
      301 cttcgccagc cccatgccgt agaggtattt catggcggcg aacatctcgc gcattccggg
      361 gccgcccttc ggcccctcgt agcggatgac cacgacggtt ccctccggta tctccccggc
      421 gaggatggct tcgtcggccg cgtcctcgcc gtcgaacacc ctggccgcgc cggagaagac
      481 gcgcatggac gggtcgatag ccgcaggctt cgtgatcgcc gtgtccggag ccagattgcc
      541 gcgcaggacg gcgagaccga ggttgacgcc gaacgggtcg gacgcggggc ggatgatctc
      601 cggattcgcc ggatagcgct cgacatgcgc gtcgaggttc tcgccgacgg cggagccggt
      661 gaccgtgacg cagtccgtat ggaggagcgc gcggatctcc ttcatgaccc tcggaattcc
      721 tccggccaga tggaacgcct ccatgtcgtg cttcgacgcc gggttcacct tcgccacgtg
      781 cggcgtcgtc cggctgcatc gttcgtagag cgccatcatc tcctcggcgg gaatccctat
      841 ctcggcggcg atggccgaaa ggtggagcac cgcgttggtg gagcctcccg tcgccatcag
      901 cacgcggacg gcgttctcca gagaatccct cgtgatgatg tcgtgcgcgc cgatcccctt
      961 ctccacgagc gcgacgatcg ccttgcccgt ctgttcggcg aggcggagtc gctcggcgta
     1021 gaccgccggg acgagcgcgc cgtcgggaat ggtcattccc agagcttcgg agagacagca
     1081 catcgtgttc gccgtcccga ggaaggagca ggagccgcag gtggggctgc agagcccttc
     1141 gaggttgcgg taggtcttct cgtcgatcag cccgcgctgg agcatgccga aggcctccgc
     1201 gttcgtggtg ctgtcggact ttcttccgtc gaactcaacg ccgccgagca tcgggccgcc
     1261 ggggaggaga atggccggga ttccgagccg ggccgccgcc atgagcattc cggggacgat
     1321 cttgtcgcac gacccgagca gaacgatgcc gtcgaggctg tgcgcccgca ccatcatctc
     1381 cacgtcgttc gcgatgagtt ccctggaggg gaggatgtac ttcatcccct cgtgcccctc
     1441 ggagatgccg tcgcatccgg cgatgacgcc gaactccgca acggcgccgc ccgccttgtg
     1501 gacgcccttc cggacgtact cggcgatctg ccgaaggttg aagtgccccg gcacgagttc
     1561 gttccacgag ttggcgatgc cgatgatcgg ccccgagagg tccgagtcgg aataccccat
     1621 cgatttgaaa aggttcctgt ggttcgcatc cttcgtcgcg ttcgcgctgt gcagcttcat
     1681 agcgtccctc ctcaataatg gcgttcgtgt ttataacatg cttcgcgcgc tcctcacccc
     1741 agcagggcgg ggttcatcct ccagaccgtg aaggcgagcg cggcggcgaa ggtcgtccac
     1801 gcgaggtacg gagccagcag ccacgcggcg agcgggcgaa cggcgtgaaa cgccttcatc
     1861 gtggcaacga tgagcgccca gaggacgagg atctcgacga aagcccacat cccctgccgc
     1921 cagacgaaga agatccacgt ccagagcgcg ttgagcacaa gctggtcgat gaagagagag
     1981 agcggaacga gccccccgcg gaagcccctc tcgcgccaga cgagccacgc ggcgacgccc
     2041 atcatcgcgt agagcgccgt ccacacgggg ccgaagagcc acccgggagg cgcccacgaa
     2101 ggtcgcgcca gctccttata gaacgaggcg gattccaccg aggcaagccc cccgacggcc
     2161 gccgccgcga agacgattcc gagccagaac agcagtccga tacttttcga catcattcct
     2221 ccccctttcg attcgccgtc cattcgctcg ccgattctcc gcgaagctgc cggaactatc
     2281 cggcgaatcc cgcataagtg tacttcctct tccccgtccg gtccagagcg ccttatcggg
     2341 aggaggggca ctcttcgccg gctcttttat gtgcggcgag cgcgttgggg ttgtgcgcga
     2401 cataagagga cgacgcaggc gctcaacgca cgtcgtctca tcggcagctt cgcgtgcaaa
     2461 aacaacctcc ggcgtataaa atagaggtgt tcggcacacg cataaaagaa cggctcgcag
     2521 tagaatgttt taaaagcggt tcctgcgcag gcttgggaga ggttgcgtgc aactcaggac
     2581 aggaggtgtt ttcatggagg ccgcgacacc gtacgaaacc atactctatc tcgcccgcaa
     2641 atacatctgg tggaagactc ctgaagaagc cgccgccatg cctgagcgtg ttctggccca
     2701 ggtgatgaac atcggggact atgacgacgt gcttctgatg aaatcccgct tcggggacga
     2761 tgcgctccgc aacgtgatcg cgcacgccga agcagggcag ttcaacgcca ggtcatggca
     2821 ctactggcat tacatgctcg ggctcgcaga gatcgaccac gtccctccgc tcccgacaag
     2881 acgcttttcg tggagagggg cggggaaaac tacttcttga agaggtcgga gtgggttccg
     2941 gtgcgctcca gtgtcagaac gagcgattct tccgtgatct tgtagatgag aagccagtcg
     3001 ggacgtatgt ggcattctct atactccgcg tagtccccgg acaatgtatg atccctgtat
     3061 tgcctcggaa gagtctcccc gcttgccaat gctcgtatga catcggcgag aagggagata
     3121 tcgcatcccc ttctttcgac caacgcgaaa tccctcttga accgcgaggt tctctggatt
     3181 tcgtatcgga gcgtcatgac ctcagttcgg tcagaaggtc ttcgacggtc gaaaaacgca
     3241 acgcgtcggg gtctttcagc atccgctcgc cttcttccag cgcggctacg gtctcttcgt
     3301 tggggcgctc ctcctcgagg gcgtcgatgt agccaagcac ccgcatggca ttccgctccg
     3361 agagtttgcg gaaacggcga tacagttctt ttttggcgga aatttcaagt gtagtccgag
     3421 gcattggtat ccccccttcg cgtaggagta ttgtacagcg aattccggca cgcttcaacg
     3481 ccggggagac ctccgcgtta ctgcgtcagc cagcggtact cctgcctgct ttcccgctat
     3541 aaaaggcaat cgcaacttcc ggcagtacga cttcacagga atctccgcgt gcaaaaaacc
     3601 cccgcaacat atagaattgt tctcgtgaag tcgacctcca tccatcggtc gacgcgcttc
     3661 tcttgaatcg aggtctcaag gaggtatcgc aatgaccgaa ttctatgcgg tgatagacag
     3721 agacgaggac ggtcaatttt tcgcctacgt cccggagctt ccgggctgcc atacgtgcgg
     3781 cgagaccatg gaggagcttc tggagaatct tgaagaggcg atcgcgctgt acctcgaggt
     3841 tgtggagcat ccccgcgagt ttcccagcat ccggaaagta gccgtcggcg agccccctca
     3901 gatgctcttc ccgatatccc gcgccttctc cagcgctccg gtggacacga cgtccccctt
     3961 gttgctgatc ccgcgcacgc cgagcgagcc gcggtcctt
//
LOCUS       DAOPKU010000259         3992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24890_length_3992_cov_5.035052, whole genome shotgun sequence.
ACCESSION   DAOPKU010000259 DAOPKU010000000
VERSION     DAOPKU010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24890_length_3992_cov_5.035052"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PK849_08750"
     CDS             complement(<1..235)
                     /locus_tag="PK849_08750"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPE66253.1"
                     /translation="MRYIALLGSPRKDGNTAALLASFLEGAASKGYSGELIRLHEKKI
                     LPCAGCNACKKEGKCVLSDDMTDLYPLVEEADVI"
     gene            465..1307
                     /locus_tag="PK849_08755"
     CDS             465..1307
                     /locus_tag="PK849_08755"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPE66254.1"
                     /translation="MNEIRSIRRIVTGKPATDGAGVKLVRVIGYNDTQDFDPFLMLDA
                     FDSTNPEDYVKGFPWHPHRGIETITYLIRGDIAHGDSLGNKGRILDGECQWMTAGSGI
                     LHQEMPQPSERMLGTQLWLNLPAKDKMTEPKYNDIVRENVPTVAEEGLNIRIVSGNYK
                     GTDGAFRGEYVDALYLDAEMAPGAEWLLETDPEATLFIYIVQGAGLFGPSGEAVAEKH
                     AVLFEKGNAFRAKASEAGARIFLMAAKPLGEPIAWGGPIVMNTREELDLAFRELQEGT
                     FIKR"
     gene            complement(1368..1676)
                     /locus_tag="PK849_08760"
     CDS             complement(1368..1676)
                     /locus_tag="PK849_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPE66255.1"
                     /translation="MALRLKWSPDALEDLETIAEYIARDSSSYASSVVSKIVTVAKTI
                     PDFPRSGRIVPEIGDHAIREHVVFSYRLIYRVEPDHITLLAVIHGKRLLEKNPAWCDE
                     "
     gene            complement(1661..1849)
                     /locus_tag="PK849_08765"
     CDS             complement(1661..1849)
                     /locus_tag="PK849_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66256.1"
                     /translation="MIAVKDEVRTLLERLPDDCSFEDIQYHLYVLEKVRRSAARVEQD
                     GALEHDEVEKRLHRWLSV"
     gene            complement(2135..3262)
                     /locus_tag="PK849_08770"
     CDS             complement(2135..3262)
                     /locus_tag="PK849_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HPE66257.1"
                     /translation="MERKLAVAQFGCGKMSAYTMRYVYEKGAEIVAAFDMNPAVVGKD
                     IGTIMGTEKKGVTVLDAKMADETLAALKPDACIITTMSLMKDLREAMLVCAKNGVNAI
                     STCEEAFYPWNSSRAVTEEIDELAKQNNCTICGAGYQDVFWGNLIATLAGATHSIRTI
                     RGKSSYNVEDYGIALAQVHGAGLTLDAFEKEIASADNISEAERKALIEKGEFLPSYMW
                     SVNGWLCERLGLTPVSQTQKCVPHTHTSELHSKTLGMTIPAGHATGMSAVVTTETEEG
                     VTLETECIGKVYAPDEFDRNDWTIVGEPDTQVVINRPATVELTCATIVNRLPDIVNAP
                     AGYVTTDKMPTNSYRVKPLHTYVDAEALQSSSSKRAVWHDE"
     gene            complement(3363..>3992)
                     /locus_tag="PK849_08775"
     CDS             complement(3363..>3992)
                     /locus_tag="PK849_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66258.1"
                     /translation="REQLDTQDVAVVEKLLEKQKARLDEELLRLSATRRCVERTLINY
                     KRYHSAPRDGMIVVEFIPDRAIYCYDSGINFYDYGLETYEYILRELKGHIALNALPMA
                     YFCNVGTVLRKPLLLRKEFVSTEVFIFVDDDFQQSPNVETLPSGTYLCMSCDSFHKEK
                     EYAGKLLAHAEENGYEIAGDYICEVIADLPVFHNNERGMFIKLQVPILF"
BASE COUNT          787 a         1152 c         1185 g          868 t
ORIGIN      
        1 cgatgacgtc ggcctcctcg acgagaggat agagatcggt catgtcgtcc gagagaacgc
       61 acttcccttc ttttttacag gcgttgcacc ccgcgcacgg gagtattttc ttctcgtgga
      121 gccggatgag ctcccccgaa tatcctttgg aagccgcgcc ttcgagaaac gaggcgagca
      181 gagcggcggt gttcccgtct ttcctggggc tcccgagtag tgcgatgtag cgcatggcat
      241 catctccttc agatagggta ggaaatagta ttgcaactgg acaaagacca cgaagggacc
      301 ggacacacct cggcggatcg ttgccttcac gtaaggatac acgatgtttt ggtaaatttc
      361 cacacaactc cgtcggcgag ccgcaagggg cttcccggtg tgcttccaat gaaatgctat
      421 aatcgcaatg agcatacggt gcagccgaag gagggtaaca agttatgaac gaaatacgaa
      481 gcatccgcag aatcgtcaca ggaaaacccg cgacggacgg cgcgggggtg aagctggttc
      541 gtgtcatcgg gtacaacgac acgcaggatt tcgacccgtt cctgatgctg gacgcattcg
      601 actcgacgaa cccggaggac tacgtcaaag gcttcccctg gcatccgcac cgggggatcg
      661 agacgatcac ctatctgatc cggggcgata tcgcgcacgg cgacagcctc ggcaacaaag
      721 ggcgcatcct cgacggggag tgccagtgga tgacggccgg atcgggcatc ctccaccagg
      781 agatgccgca gcccagcgag cggatgctgg gaacgcagct ctggctcaat ctgcccgcga
      841 aggacaagat gaccgaaccg aagtacaacg acatcgtcag ggagaacgtc ccgacggtgg
      901 cggaggaggg gctgaacatc cgcatcgtct ccggaaacta caaggggacg gacggggcct
      961 tcaggggaga gtacgtggac gccctctacc tcgacgctga gatggcgccg ggggcggagt
     1021 ggctgctgga gaccgatccg gaagcgacgc tcttcatcta tatcgtacag ggcgcggggc
     1081 tcttcggccc ttccggggag gccgtcgcgg agaaacacgc cgtgctcttc gagaagggga
     1141 acgcgttccg cgcaaaggcc tcagaagcgg gcgcgcgcat ctttctgatg gccgcgaaac
     1201 cgcttgggga gccgatagcg tggggcggtc cgatcgtgat gaacactcgc gaagagctgg
     1261 acctggcgtt ccgcgagctt caggagggga cgttcatcaa gagatagccg ttccagaaac
     1321 tccgggcttt tttgccattc gaccatatgc acactggtgt aacggggcta ctcgtcgcac
     1381 catgcggggt ttttttccag caatcgcttt ccatggatca cggcaagcag cgtgatatga
     1441 tcgggttcga cgcgatagat caggcgataa ctgaaaacta catgctcgcg tatcgcgtga
     1501 tcgcctattt ccgggacaat tcgcccgctc cgcggaaaat cggggatagt ttttgcgaca
     1561 gttacaattt tggatactac agaagaggcg tacgaagacg agtctctcgc gatatactcc
     1621 gctatggtct cgaggtcttc cagcgcgtca ggagaccatt tcagacggag agccatcgat
     1681 gcaatctctt ctcaacctcg tcatgctcca atgcaccgtc ctgttcgact ctcgcagcgc
     1741 ttcttctaac tttttcaaga acatagagat ggtactgtat atcttcaaag gaacaatcgt
     1801 cgggaagccg ctccagaaga gtgcgcactt catcttttac tgcaatcatc gaggttcacg
     1861 ctccttttcg gtcccttgct cccgctcaag ggggaacttc tcataacacg accggacgcg
     1921 ggagatcgcc gcgaagttcc gatccggcga attttcttga tttattgtat cacgatgatc
     1981 gcagggcttt tcgttccttc ggcttctttc atcgctcgaa aaactgcgaa gaatcagctt
     2041 gggaaacgcc tgtctcaaaa agcatgcggc cccgccttgg tcggagccgc atgctttttg
     2101 aaaaaaccgg tatcaccgca ctcgcggaat cgctctattc gtcgtgccac acggcccgct
     2161 tcgacgagga cgactgaagc gcttcagcgt cgacgtacgt gtgcagcggc tttactctgt
     2221 aggaattggt cggcatcttg tcggtggtca cgtacccggc cggagcgttg acgatgtcgg
     2281 gcagccggtt gacgatggtc gcgcacgtca gctcgaccgt cgcggggcga ttgatgacca
     2341 cctgcgtgtc cggttcgccg acgatcgtcc agtcgttccg gtcgaactcg tcgggcgcgt
     2401 acaccttccc gatgcactcc gtctccagcg tgactccttc ctcggtctcc gtcgtcacga
     2461 cggccgacat gccggtcgcg tgtcctgccg ggatggtcat gccgagggtc ttcgagtgca
     2521 gctcggacgt gtgcgtgtgc ggcacgcatt tctgcgtctg cgaaacggga gtcagcccca
     2581 ggcgttcgca cagccacccg ttgacgctcc acatatagga gggaaggaac tctcccttct
     2641 cgataagcgc cttgcgctcc gcttcggaaa tgttgtcggc agaggcgatc tccttctcga
     2701 aggcgtccag cgtcagcccg gcgccgtgca cctgagcaag cgcgatgccg tagtcctcca
     2761 cgttatagct ggacttgccg cgtatcgtgc ggatgctgtg cgtcgctccc gcgagcgtgg
     2821 cgatcaggtt gccccagaag acgtcctggt accccgcgcc gcaaatcgtg cagttgttct
     2881 gtttcgcgag ctcgtcgatc tcttcggtga cggcgcggga ggaattccac gggtagaagg
     2941 cctcttcgca cgtgctgatg gcgttgacgc cgttcttggc gcacacgagc atggcctccc
     3001 gaagatcctt catcaggctc atggtcgtga tgatgcacgc gtcgggcttc agcgcggcga
     3061 gcgtttcgtc ggccatcttc gcgtccagca ccgtgacgcc cttcttctcc gttcccatga
     3121 tcgtaccgat atccttgccc acgactgccg ggttcatatc gaacgcggcg acgatctccg
     3181 cgcccttttc gtagacgtag cgcatcgtgt aggcggacat ctttccgcat ccgaactggg
     3241 cgaccgcgag tttcctttcc ataacgcaat acccccctga acgtagagtg tgttgtggtt
     3301 cttccacgtg ttcttctttt cactataggg tctctacatg gtatagagtc aaggggtgcg
     3361 cgttaaaaaa gtatcggaac ctgcaacttg atgaacatgc ctcgctcgtt gttatggaaa
     3421 accggcagat cggcgatcac ctcgcagatg tagtcgcccg cgatctcgta cccgttctcc
     3481 tcggcgtgcg caaggagttt tccggcgtac tccttctcct tgtggaagct gtcgcacgac
     3541 atgcagaggt acgtcccgga gggcagagtt tcgacgttcg gcgactgctg gaagtcgtcg
     3601 tcgacgaaga tgaacacctc ggtggagacg aactccttcc tcagcagaag cggttttctg
     3661 agcacggtcc ccacgttgca gaagtaggcc atggggagag cgttgagcgc gatgtgccct
     3721 ttcaactccc ggaggatgta ctcgtacgtc tccagtccgt agtcgtagaa gttgatgccg
     3781 ctgtcgtagc agtagatcgc gcggtcgggg atgaattcga cgacgatcat gccgtcccga
     3841 ggggcggagt ggtagcgctt gtagttgatc agcgtgcgct cgacgcatct tctggtggcg
     3901 gagagacgaa gcagttcctc gtcgagtctg gccttctgct tctcgagcag cttctcgacg
     3961 acggcgacgt cctgcgtgtc gagctgctcc ct
//
LOCUS       DAOPKU010000260         3983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_24967_length_3983_cov_4.764002, whole genome shotgun sequence.
ACCESSION   DAOPKU010000260 DAOPKU010000000
VERSION     DAOPKU010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24967_length_3983_cov_4.764002"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PK849_08780"
     CDS             <1..592
                     /locus_tag="PK849_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HPE66259.1"
                     /translation="VPDGGDVLLGGEPLPPGRPKTAVDRKMARIPEDRMTTGLLLELT
                     VEENLVLENHASFRSFGMLDHAAIGRHADRLIGEFGITTDGRRAVARTLSGGNLQKII
                     LARELSASPRAVVAAQPTRGLDVGAIEYVHKRILEARESGAAILLISEDLDEIFLLAD
                     RIAVMYEGRIMGVAERDEASREQVGLWMSGVNEPCA"
     gene            589..1641
                     /locus_tag="PK849_08785"
     CDS             589..1641
                     /locus_tag="PK849_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66260.1"
                     /translation="MIVSKRAPLPGWIQLLVPVGAVLATLLLSAIPILIAGGDLWLSY
                     ASLFKGALGTRYNFLETCVKAAPLTFTGLAVAFAFRAKFWNIGAEGQLLAGAIAAAWV
                     GIYAPPLPKVIVLFLVCAAGFLAGGAWATIPALLKTKYRVDDVVTTLLLNYVMWHLMG
                     YLLFGPLQMPGSSWPRSPAVAEIARFPVLLIRSRFHLGIVLALVAVLVVWFVNSKTVF
                     GYQSRAVGVNPRAAAFGGINVNAVIMKTALLSGGLAGLAGVGEVVAIHFHLLMDISPG
                     FGYSGIVIAMLGRLHPVGTVLSAFFFSVIIVGAQSMSRLTGVPTYIAEVIQGMALIVM
                     LIALLLTEYRVKAVRD"
     gene            1641..2585
                     /locus_tag="PK849_08790"
     CDS             1641..2585
                     /locus_tag="PK849_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66261.1"
                     /translation="MEQVMTTAFITGLLAAMMRMATPIIFGTLGEILCERAGVLNLGI
                     EGIMLMGAMTGFLTTLTTGSLWLGVAAAALVGVLLSLFMAFLAVYLGLSQHVSGLGIT
                     LFSTGLAMFIYRLAVGSPVNPPTVRPFTQTAIPFLSGIPILGTALFNQYVLVYIALLL
                     VPLIWFLFYRTSWGLAIRTVGENPFAADTVGINVNLTRTLCLAAGGALMGVGGAFLTL
                     AHQNMFLIDVVGGRGWIAIAMVIFGNWDPAKGMAGALIFGFLDALQLRLQGLGFDIPF
                     HLFLIIPYLMTVIALVGVSRRAAAPAGLLKPYRREEKG"
     gene            2737..>3983
                     /locus_tag="PK849_08795"
     CDS             2737..>3983
                     /locus_tag="PK849_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPE66262.1"
                     /translation="MTLQHVGKSVPRVDAVRKATGEAVFVADIVLPRMLHAKVLRAGV
                     PHAKILSIDTSKAEAMPGVKKVVTGQGCGMLFGTCLWDQPPLAVDKVRHAGEPVAVVL
                     AETGNQAAAALKEIAVEYKKLPFVLDPVEAAAPGAPLIHEKNGEYRRVEYSVHPIPGS
                     NVFHHYKLRKGNEQDGFAKADVTVEDEFEFPLSSQAPLEPHGAICRFGTDGDLEIWAS
                     NQAPFVLRDVLADMFGIPATKVRVHIPYLGGGFGGKSDVSIEPMVAYAASFVPGYAVK
                     LVLTRGEVISGALIGRGMKGRMRIGAKLDGTFTALKAEMYFADGAYGDTGWPVDTVAG
                     HNCTGPYEFPNAIVDVYGVYTNSPIVGAYRGYGHPEGQFMSSRLIDMLARKLDMTPAE
                     IMRKNFLCEGRKNALGQTIKKSNG"
BASE COUNT          634 a         1326 c         1303 g          720 t
ORIGIN      
        1 cgtccccgac ggcggcgacg tgcttctcgg cggagaaccg cttcctcccg ggaggccgaa
       61 gaccgcagtg gaccggaaga tggcccgtat cccggaggac aggatgacca cgggactgct
      121 gctcgaactc accgtggagg agaacctcgt cctcgaaaac cacgcctcct tccgttcgtt
      181 cggcatgctc gaccacgcgg caatcggcag gcacgcggac aggctgatcg gcgagttcgg
      241 catcacgacc gacggacggc gcgccgtcgc ccggacgctc tccgggggca acctgcagaa
      301 gatcatcctc gcccgcgaac tatcggcctc cccgcgcgcc gtcgttgcgg cccagccgac
      361 gcgcggactc gacgtcgggg ccatcgagta cgtccacaag cggattctgg aagcccgcga
      421 gagcggcgcg gccatcctcc tgatctcgga ggaccttgac gaaatcttcc tgctcgccga
      481 ccggatcgcc gtgatgtacg aagggcgtat catgggcgtc gccgagcggg atgaagcgag
      541 cagagagcag gtcggtctct ggatgagcgg ggtgaacgaa ccgtgcgcat gatcgtgtcg
      601 aagcgggcgc ctctgcccgg atggatacag cttctcgtgc cggtgggggc cgtcctggcg
      661 acgcttttgc tctcggctat ccccattctg atcgcgggag gcgacctgtg gctctcgtac
      721 gcctcccttt tcaagggggc gctgggaacg cgctacaact tcctggagac ctgcgtgaag
      781 gcggcgccgc tgaccttcac cgggcttgcc gtggccttcg ccttccgggc gaagttctgg
      841 aacatcgggg cggaaggaca gctcctcgcc ggcgccatcg ccgccgcctg ggtcggcatc
      901 tacgcgcccc cccttccgaa ggtgatcgtc ctttttctcg tctgcgccgc agggtttctc
      961 gccggaggcg cgtgggcgac gatccccgcg ctgctgaaga cgaagtacag ggtggacgac
     1021 gtggtcacca cgctgctgct gaactacgtc atgtggcacc tgatgggata cctgctcttc
     1081 ggtccgctgc agatgccggg gtcgagctgg ccccgctccc ccgccgtggc ggagatcgcg
     1141 cgcttcccgg tgctgctgat ccgctcgcgc ttccacctgg gcatcgtcct cgccctcgtc
     1201 gccgtgctcg tcgtctggtt cgtgaacagc aagacggtct tcggctacca gtcgcgcgcc
     1261 gtcggggtga acccgagggc ggcggccttc ggcggcatca acgtgaacgc cgtcatcatg
     1321 aagaccgcgc tcctctccgg ggggctcgcc gggctcgcgg gcgtgggcga ggtggtcgcg
     1381 atccacttcc acctgctgat ggacatctcc cccggcttcg gctactccgg catcgtcatc
     1441 gccatgctcg ggcggctgca cccggtcgga accgtcctct ccgcgttctt cttcagcgtg
     1501 atcatcgtgg gggcgcagtc gatgagccgc ctgaccggcg tcccgaccta catcgcagag
     1561 gtgattcaag ggatggcgct catcgtgatg ctcatcgccc tgctgctgac cgaataccgc
     1621 gtaaaggcgg tgagagactg atggaacagg tgatgaccac cgcttttatc acgggacttc
     1681 tggcggccat gatgcgcatg gcgacgccca tcatcttcgg cacgcttgga gagatcctct
     1741 gcgaacgcgc cggggtgctc aacctcggta tcgagggaat catgctgatg ggggcgatga
     1801 cgggctttct cactacgctg accacgggct cgctctggct cggcgtagcg gccgcggcgc
     1861 tcgtcggcgt gctgctctcc ctcttcatgg ccttcctcgc cgtctacctg gggctctcgc
     1921 agcacgtctc ggggctcggg atcacgctct tctcgacagg gctggccatg ttcatctaca
     1981 ggctcgcggt cggctccccg gtgaacccac ccacggtgcg ccccttcacg cagacggcga
     2041 ttccgttcct ctcgggcatt cccatcctgg ggacggctct cttcaaccag tacgttctgg
     2101 tctatatcgc gctgctcctc gtgccgctga tctggttcct gttctaccgc acctcgtggg
     2161 ggctcgccat ccgcaccgtc ggggaaaacc ccttcgccgc ggacacggta ggcattaacg
     2221 tcaacctgac gcggacgctc tgcctcgcgg cgggcggcgc gctcatgggc gtcggcggcg
     2281 cgttcctgac cctggcccac cagaacatgt tcctgatcga cgtggtgggc ggcagggggt
     2341 ggatcgccat cgccatggtg atcttcggca actgggaccc ggcgaagggg atggcgggcg
     2401 cgttgatctt cggcttcctc gacgcgctcc agctgcgcct ccaggggctg ggcttcgaca
     2461 ttccgttcca cctcttcctg atcatcccct acctgatgac ggtcatcgcg ctcgtcggcg
     2521 tttcgcggcg cgcggcggct ccggcggggc tgctgaagcc gtaccgcagg gaggagaagg
     2581 ggtagcggaa actccgggga gcacgggcgc gttccttctg cccgtgcgtg tccggcgtca
     2641 ctacttccgg ggggcggcgc cctgcgcact tttcgggctc gctccatttc ggagcaactt
     2701 tttcatcgca tctctttgtg aaagagaggg gaaagcatga cgttacagca tgtaggaaag
     2761 agcgttccgc gcgtggacgc ggtccggaag gcgacgggag aggccgtctt cgtggccgac
     2821 atcgttctgc cgcggatgct ccacgcgaag gtgctgaggg ccggggtccc tcacgccaaa
     2881 attctctcga tcgacacgtc gaaggcggag gcgatgcccg gcgtcaagaa agtcgtcaca
     2941 gggcaggggt gcggaatgct cttcggcacc tgcctctggg atcagccgcc cctggccgtc
     3001 gacaaggtcc gccacgcggg cgagcccgtc gcggtcgtcc ttgccgagac cgggaatcag
     3061 gcggccgcgg cgctcaagga gatcgccgtc gagtacaaga agctcccgtt cgtcctcgac
     3121 ccggtcgagg cggcggctcc cggcgcgccg ctcatccacg agaagaacgg agagtaccgg
     3181 agagtggaat actccgtcca tccgatcccc ggaagcaacg tcttccacca ctataagctc
     3241 cgcaaaggaa atgaacagga cggtttcgcc aaagccgacg tcacggtcga ggacgagttc
     3301 gagttccccc tgtccagcca ggcgccgctc gagccgcacg gcgcgatctg ccgcttcggc
     3361 acggacggcg atctcgagat atgggcctcc aaccaggccc cgttcgtcct ccgggacgtc
     3421 ctcgccgaca tgttcggcat ccctgcgaca aaggtgcgcg tgcacatccc ctacctcggc
     3481 ggcggtttcg gcgggaagtc cgacgtcagc atcgaaccga tggtcgccta tgcggcgagc
     3541 ttcgtccccg gatacgccgt caagctggtg ctcacgcgcg gcgaggtcat ctccggagcg
     3601 ctgatcggcc gcgggatgaa ggggcgcatg cggatcggcg cgaagctcga cggcacgttc
     3661 accgcgctga aggccgagat gtacttcgcg gacggcgcgt acggcgacac ggggtggccc
     3721 gtggacaccg tcgccggaca caactgcacc ggcccttacg aatttcccaa tgcgattgtc
     3781 gacgtctacg gcgtctacac caattcgccg atcgtcggcg cgtacagagg gtacggacac
     3841 cccgaggggc agttcatgtc cagccgcctg atcgacatgc ttgcgcgaaa gctcgacatg
     3901 acgccggcgg agatcatgcg gaagaacttc ctctgcgagg ggcggaagaa cgccctcgga
     3961 cagacgatca agaagagcaa cgg
//
LOCUS       DAOPKU010000261         3978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_25016_length_3978_cov_4.405302, whole genome shotgun sequence.
ACCESSION   DAOPKU010000261 DAOPKU010000000
VERSION     DAOPKU010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25016_length_3978_cov_4.405302"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..262
                     /gene="secE"
                     /locus_tag="PK849_08800"
     CDS             80..262
                     /gene="secE"
                     /locus_tag="PK849_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPE66263.1"
                     /translation="MGNILTFVREARAELKKVTWPGKKQVWYSTIVVIAFTLLVSAYL
                     GIVDMVLTGVFSRLFS"
     gene            350..892
                     /gene="nusG"
                     /locus_tag="PK849_08805"
     CDS             350..892
                     /gene="nusG"
                     /locus_tag="PK849_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPE66264.1"
                     /translation="MDSIEARRWYIVQTYAGYENRVKANLEQRIATMGMEDSIFGVLV
                     PVEERVSIKEGKSKKVTRKLFPSYVLVEMRLNDQSWYVVRHTPGVTGFVGSGNHPIPL
                     TEKEVKEIMGKVGKDQAKPKIEINFRPGDVVKVKSGPFEGHVGPVVEIFPEKGKVKFS
                     VTVFGRETSVETDYTELEKL"
     gene            1001..1426
                     /gene="rplK"
                     /locus_tag="PK849_08810"
     CDS             1001..1426
                     /gene="rplK"
                     /locus_tag="PK849_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPE66265.1"
                     /translation="MAKKVIAEIKLQLPAGKATPAPPVGPALGQHGVNIMEFVKQFNA
                     KTADQAGLVIPAVITVYADRSFTFVLKTPPASVLLKKAAGLESGSGEPNKKKVATLDR
                     AKVREIAELKKVDLNSYDVDAAMRMVEGTARSMGITIVD"
     gene            1489..2199
                     /gene="rplA"
                     /locus_tag="PK849_08815"
     CDS             1489..2199
                     /gene="rplA"
                     /locus_tag="PK849_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPE66266.1"
                     /translation="MAKLSKRLTAAHQKIEDGKQYTVKEAIALFKETATAKFDESMEV
                     HIRLGVDPRHADQQVRSTVVLPHGTGKTKRVLVLAMGEKVKEAEEAGADFVGGEELVG
                     KISGGWLDFDAVIATPDMMKSVGRLGKVLGPRGLMPSAKTGSVTFDIASAIKEIKAGR
                     VEFRVDKFAIVHNAIGKKSFSNEQLFDNAKTLIQAVLKARPASVKGTYVKSLNLSSSM
                     GPGIKIDVASAQKEVAGE"
     gene            2387..2920
                     /gene="rplJ"
                     /locus_tag="PK849_08820"
     CDS             2387..2920
                     /gene="rplJ"
                     /locus_tag="PK849_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPE66267.1"
                     /translation="MPATLKYEQVDELKGRLARTQAIFACEYRGMTVAQITALRAQVR
                     AAGGEMKVAKNTLMRIAMEQEGFIPFPDDMAFGPNVFALAYGDPVAVAKALRDFAKDR
                     TNKFFVLKGCVLGQTLLDATQIGALADLPSKEVLIGQVVRTIAAPIAGLLTVLSGPAR
                     GLVTCLSQIKEKKEDVA"
     gene            2995..3375
                     /gene="rplL"
                     /locus_tag="PK849_08825"
     CDS             2995..3375
                     /gene="rplL"
                     /locus_tag="PK849_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPE66268.1"
                     /translation="MTRDEMIQAIENMTVLELSELVKALEEKFGVSASAPAMAMPMMA
                     MGAAPAAAAEEEKTEFDVIYKAPGANKINVIKVVRELTGLGLKEAKELVDNPPKAVKE
                     AVSKEEAEEIRKKLAEAGADVEVK"
     gene            3447..>3978
                     /locus_tag="PK849_08830"
     CDS             3447..>3978
                     /locus_tag="PK849_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE66269.1"
                     /translation="MIDCHVHVYPPEIIRDAAVIGKNEPYFELLSTGKVHKWATAEDV
                     IAAMDRDGIDVSWIFGFAFKDPGLCALCNEYVIEAVRKYPTRLKGLAVVPPCAHGAGN
                     EIARCREQGLVGVGEIFPGGQDFDISDVRQTWRLAGAVAEAGMFLLVHSAEPVGHDYP
                     GKGNTGPREAAEFCLHH"
BASE COUNT          975 a          974 c         1187 g          842 t
ORIGIN      
        1 aaaaccgggg gttgcaggtt cgagtcctgc cgggcctgcc atttttttat tgtcaaaaac
       61 aagatggggg gcagaggttg tgggaaatat cctcaccttc gttcgggaag cccgggccga
      121 gttgaagaaa gtaacgtggc cggggaaaaa gcaagtctgg tattcaacaa tagtcgttat
      181 cgcttttacg cttttggttt cggcctatct cgggatagtt gatatggtac ttacaggagt
      241 cttttccagg ctctttagtt aaaagcccgc tgccggtcca ggcagttgat accgggatca
      301 tttcagagat cggcagggag gtgagggggc cgcaagaccc ccttaaagta tggacagtat
      361 tgaagcgcgc cgctggtata tagtacaaac gtatgcaggg tacgaaaatc gcgtcaaggc
      421 aaatctcgaa cagagaattg ccacgatggg tatggaggac agtattttcg gcgtcctcgt
      481 ccctgttgag gagcgtgtct ccataaaaga gggaaagagc aaaaaggtga ctcgcaagct
      541 ttttcccagt tatgttcttg tcgagatgcg tcttaacgac cagtcgtggt atgtcgttcg
      601 gcatacccca ggcgtgactg gctttgtcgg ctccggaaac catccgattc ctctcactga
      661 aaaagaagtg aaagagatca tggggaaggt cggtaaagac caggcgaaac ccaaaatcga
      721 gattaatttc cggcctggcg atgtcgtcaa ggtaaaatcc ggaccctttg aaggtcatgt
      781 ggggccggtt gtcgagattt tcccggaaaa gggaaaagtg aagttctctg tgacggtctt
      841 cggccgggag acatctgtag aaactgacta tacggagctt gagaagctct agatatatat
      901 cagcgcgaag gcccgcattc cagggaggca agagaactct tggctcctgt ttttccctgt
      961 gtgcggaagt gctttcagta acgagaggag actttgagga atggcgaaga aagtaatagc
     1021 agagatcaag ttgcagttgc ccgcaggcaa ggctacgcct gcgccccccg tgggacctgc
     1081 tcttggtcag cacggagtga atatcatgga gttcgtgaag cagttcaacg cgaaaacagc
     1141 agatcaggcc gggcttgtaa ttcccgccgt gatcacggtg tacgcggaca gaagcttcac
     1201 cttcgtgctc aaaacaccgc ccgcgagcgt gctgctcaag aaggcggccg gattggaatc
     1261 cggctccggc gaaccgaaca agaagaaggt agccaccctt gacagggcga aagtgcgcga
     1321 gattgcggag ctgaaaaagg tcgatctgaa ttcctatgac gtcgacgccg caatgcgtat
     1381 ggtcgaggga actgcgcgtt caatgggtat cactatcgtc gattgatgaa aagtggtttt
     1441 gaatttgtgg gaggcctcaa ggctgtctga ccaccaggag ggaaaaaaat ggcgaagttg
     1501 agtaaaagac tgactgcagc acaccagaag atcgaggacg gaaaacagta caccgtgaaa
     1561 gaggcgatcg cgctttttaa agagacggcg accgcgaagt tcgacgagag tatggaggtc
     1621 catatccgtc tcggcgtaga tcctcgtcac gctgaccagc aggtccgcag cacggttgtg
     1681 ctcccccatg ggacgggaaa gaccaagaga gttctcgttc ttgccatggg tgagaaggta
     1741 aaagaagcgg aagaggccgg agctgatttt gtcggcggcg aagagcttgt gggcaaaatt
     1801 tccggcggat ggcttgattt cgacgccgtc atcgcgaccc ccgacatgat gaaatcggtc
     1861 ggacgcctcg gaaaggtgct tggtccccgc ggccttatgc ccagcgcaaa gaccggcagc
     1921 gtgacgttcg atattgcgtc cgcgataaaa gagatcaagg ccggacgcgt cgaattccgc
     1981 gttgacaagt tcgctatcgt gcacaatgcg atcggcaaga agagcttctc caacgagcag
     2041 cttttcgaca atgcgaagac gcttattcag gctgtcctga aagcgaggcc cgcctcggtg
     2101 aagggaacct acgtgaagag ccttaatctt tcctcttcca tgggccccgg aatcaagatc
     2161 gacgttgcgt ccgcgcagaa agaggttgca ggcgagtaac gcgcttcctt tcctctgctc
     2221 aaaacgaaat atgaacttga actgaagaca gcgggtccct taaggggaca aaggccaggc
     2281 cgcccgccga ggttccggtg agtcgatcgg catctgctgc cgtacaggta ctcaatcccg
     2341 ggcccggcgc ccgggatttt ttactttcac cgaaggaggt gagtacatgc cggcaacact
     2401 taagtacgaa caggtagatg aactgaaggg gcgtcttgcc cgcacacagg ctattttcgc
     2461 atgcgaatac cgcggaatga ctgttgcgca gattacggcg cttcgcgccc aggtgcgcgc
     2521 tgcaggcgga gaaatgaagg ttgcgaagaa tacgcttatg cgtattgcca tggagcagga
     2581 ggggtttatt ccattccccg acgatatggc gttcggaccg aacgtcttcg cgctggcgta
     2641 cggggatcct gtcgcggtag cgaaagctct tcgtgatttc gcgaaggaca gaaccaacaa
     2701 gttttttgtc ctgaaggggt gcgttcttgg acaaaccctt cttgatgcga cacagattgg
     2761 cgctctcgcc gatctcccgt ccaaggaagt ccttatcggt caggtcgtcc gtaccatcgc
     2821 ggccccgatt gcgggtctgc ttacggttct ttccggtcct gcaagaggac tggtcacctg
     2881 cctcagccag atcaaggaaa agaaggaaga cgtcgcatag tttttgggcc ggaagcacat
     2941 cgcatacacg gtatgcgata cacaagatga tatatatcca aggaggaaaa aagaatgacc
     3001 cgcgatgaaa tgattcaggc tatagagaac atgacagtgc ttgagctctc cgagctcgtg
     3061 aaggctcttg aggagaagtt cggagtttcc gcttccgctc ccgcgatggc gatgccgatg
     3121 atggcgatgg gcgcagcccc cgccgctgcc gccgaggaag agaagaccga gttcgacgta
     3181 atctacaagg cgccgggcgc caacaagatc aacgtgatca aggttgtccg cgaactcaca
     3241 ggccttgggc tcaaagaagc caaggaactt gtcgacaacc cccccaaggc agtcaaagaa
     3301 gcagtcagca aggaagaggc agaggagatc aggaagaagc tcgccgaggc cggagccgac
     3361 gtcgaggtta agtagttcgc ctttcctttt ttctggtata ataaagacgg tgcgaaagca
     3421 tcgtcttttt ttatgggggg tatctcatga tcgactgtca tgtacacgtc tatccgcccg
     3481 agatcatcag ggatgcggca gttataggga aaaatgaacc gtatttcgaa ctcctctcga
     3541 cgggcaaggt acataaatgg gccacggcgg aggacgttat cgccgcgatg gacagggacg
     3601 gcatcgacgt ctcgtggatt ttcggtttcg ccttcaaaga tcccggactc tgtgcgctct
     3661 gcaacgagta tgtgatcgaa gctgtacgaa agtacccgac acgcctgaag gggcttgccg
     3721 tggttcctcc gtgcgctcac ggcgcaggga atgaaatcgc ccgatgcaga gaacaaggtc
     3781 ttgtaggcgt cggagaaata tttcccgggg gccaggattt cgatatctcc gatgtccgcc
     3841 agacgtggag gcttgccgga gcagttgccg aagcggggat gttcctgctt gttcattccg
     3901 ccgagcccgt cggccatgat taccccggaa agggaaacac cggtccgcgg gaagccgcag
     3961 agttttgttt gcatcatc
//
LOCUS       DAOPKU010000262         3953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_25217_length_3953_cov_4.474859, whole genome shotgun sequence.
ACCESSION   DAOPKU010000262 DAOPKU010000000
VERSION     DAOPKU010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25217_length_3953_cov_4.474859"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PK849_08835"
     CDS             <1..547
                     /locus_tag="PK849_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE66270.1"
                     /translation="GEPLAATGWYRVPEITEPDPETGMGNAAGSWTFGAQGCALRVDK
                     QTGEVKILHFASAFDVGRAVNPAATRGQVTGGVVQGMGAALMEKILFTDEGVAKNVTF
                     PAYKIPRLHDAPEKQTVVLLETPNEEGPWSAKPMAEHPIVAVAPTILNALRDATGVEF
                     TAIPVTPERILEALKTRKEDK"
     gene            549..1025
                     /locus_tag="PK849_08840"
     CDS             549..1025
                     /locus_tag="PK849_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPE66271.1"
                     /translation="MRVLNFTVNGTPRTVHLEEGRVMLASVLRDSLGLTGTKVGCGTG
                     QCGSCTVVMDGKAVTSCTVPAEKAEGTSVLTIESLAEGGVLHPIQQAFVEAGAIQCGF
                     CTPGIVMRLYALFTQNPDADDETIMKELDKHLCRCTGYENIVKAAHLAQEKLRAAR"
     gene            1081..2289
                     /locus_tag="PK849_08845"
     CDS             1081..2289
                     /locus_tag="PK849_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE66272.1"
                     /translation="MAFSLLIKNARTRFSQGKTLSIGIEGEKIAAVGENLPETGAAQI
                     IDAGGKLVTESFVNGHLHLCKVYTLEMVGQDALSSYHGGSMGGAMTAIEQASRVKDHY
                     DEKWIIENVRKACRLAQKYGNTHIRAFADTDTKAKLEGIKALLKAREEFKDTVDLQVV
                     AFPQDGVVRDPGADGYIRSALELGADVVGGIPWIEYTDADMQAHIDSMFALAKEFGKD
                     VSMLIDDAGDSGLRSLEMLAVKTIKEGWQGRVTAQHCRAMAMYQEPYFRKVLALLQKA
                     KIGLVSDPHTGPLHARVRDLYDAGVAVALGQDDIADAYYPFGRNNMLEIAFLAVHLLW
                     MTTFSDMEILYDLITTNAAKAMGIKGHVLEPGGNADLVVLNAEDVYHAIWEHDAPLHV
                     IRKGKDVTLK"
     gene            complement(2436..3245)
                     /locus_tag="PK849_08850"
     CDS             complement(2436..3245)
                     /locus_tag="PK849_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPE66273.1"
                     /translation="MTIFQRVDRLKAAIDGVRPFEGAMLDEIRRYYRIGLTWSSNAIE
                     GNTLTESETKVLLEDGLTAGGKPLRDSFEAIGHAEAYDFMFSLLQSASISEEDARTMH
                     RMFYRNIDESAAGSYRSRAVLITGSSYPVTEPGRIPSEMASLFEWANSERGGYHPVEF
                     AAQLHKRFVFIHPFIDGNGRISRLLMNAALIQDGYLPALIPPILRREYIDLLERARTD
                     DRPFAEFIAARVLESEKEMLRLLHIPEPEGRYDLDGLVSGITKENGHGELD"
     gene            complement(3282..3755)
                     /locus_tag="PK849_08855"
     CDS             complement(3282..3755)
                     /locus_tag="PK849_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPE66274.1"
                     /translation="MNTPDRYRYPAVLGYDASTNQYYVLWPDLPGCTTTGATEDEALE
                     HAREAMSLHLWGMEVDGDEIPAPTPLHRLDLGEYVEEGEKIVAVLVEVWMPSFRERME
                     TKAVNRTVTLPGWLDSQAKRAALNYSQILQDGIMERLKISRKVVQRKKSGAKSLV"
     gene            complement(3752..3952)
                     /locus_tag="PK849_08860"
     CDS             complement(3752..3952)
                     /locus_tag="PK849_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HPE66275.1"
                     /translation="MIKILEADGWQLHGAEGSHYQFKHPAKKGKVTVKHPDKSVPGPT
                     AKSIWKQAGLLQPPSSRKETES"
BASE COUNT          765 a         1273 c         1227 g          688 t
ORIGIN      
        1 cggcgaaccg cttgccgcga ccggatggta ccgcgttccg gagatcacgg agcccgaccc
       61 cgagaccggc atggggaacg ccgccggaag ctggaccttc ggcgctcaag ggtgcgcgct
      121 ccgcgtcgac aagcagaccg gcgaggtgaa gatactccac ttcgcctccg cgttcgacgt
      181 cggcagggcg gtcaatcccg ccgcaacgcg cggacaggtc acgggcggcg tcgttcaggg
      241 aatgggcgcg gcgctgatgg agaagatcct cttcaccgac gaaggcgtgg cgaagaacgt
      301 caccttcccg gcgtacaaaa ttccgcgtct ccacgacgcg ccggagaagc agaccgtggt
      361 gctgctggag acgcccaacg aagagggacc gtggagcgcg aagcccatgg cggaacaccc
      421 catcgtcgcg gtggcgccca ccatcctcaa cgcgctgcgc gacgcgacgg gcgtcgagtt
      481 cacagcgatt ccggtcacgc cggagagaat actggaagcc ctgaaaaccc gaaaggagga
      541 caaatagcat gcgcgtcctc aacttcacag tcaacggaac accccggacg gtccatcttg
      601 aagaggggcg cgtgatgctc gcctccgtgc tgcgcgactc gctcgggctg acgggaacca
      661 aggtggggtg cggcacgggg cagtgcggct cgtgcaccgt ggtgatggac gggaaggccg
      721 tgaccagctg caccgtcccc gcggaaaagg cggaagggac gagcgtactc accattgagt
      781 ccctcgccga gggcggcgtc ctccacccga tccagcaggc attcgtcgag gccggggcca
      841 tccagtgcgg cttctgcacg ccggggatcg tcatgcgcct ctacgcgctc ttcacccaga
      901 atccggacgc cgacgacgaa acgatcatga aagagctcga caagcacctc tgccgctgca
      961 ccggctacga gaacatcgtc aaggcggcgc acctcgcgca ggagaagctc cgcgcggcac
     1021 ggtgagcggg ctggcgacgg aacgctttca ggcagcaaaa aatgaaagga gaatgaactc
     1081 atggcattca gtcttctgat caagaacgca cggacccgct tttcacaggg aaagaccctc
     1141 tccatcggca tcgaggggga gaagatcgcc gccgtcggcg agaacctccc ggagacgggc
     1201 gctgcgcaga tcatcgacgc gggcgggaag ctggtcacgg agtccttcgt gaacggccat
     1261 ctccacctgt gcaaggtgta cacgctggag atggtggggc aggacgcgct ctcctcctac
     1321 cacggaggga gcatgggcgg ggccatgacg gccatcgagc aggcctcgcg cgtgaaggac
     1381 cactacgacg agaagtggat catcgagaac gtccgcaagg cgtgccgcct ggcccaaaaa
     1441 tacggcaaca cccatatccg cgccttcgcc gacaccgaca ccaaggcgaa gctggagggg
     1501 atcaaggcgc tgctgaaggc ccgcgaggag ttcaaggaca ccgtggacct ccaggtggtc
     1561 gccttcccgc aggacggcgt ggtgcgcgac ccgggcgcgg acgggtacat tagatcggcg
     1621 ctcgagctgg gagccgacgt cgtgggcggc attccgtgga tcgaatacac cgacgccgac
     1681 atgcaggcgc acatcgactc gatgttcgcc ctcgccaagg agttcggcaa ggacgtctcc
     1741 atgctgatcg acgacgcggg cgactccggg ctccgctcgc tcgaaatgct cgccgtcaag
     1801 acgatcaagg aggggtggca ggggcgcgtg accgcgcagc actgccgcgc catggccatg
     1861 taccaggagc cctacttccg caaggtgctc gcactgctgc agaaagcgaa gatagggctc
     1921 gtcagcgacc cgcacaccgg cccgctccac gcgcgcgtgc gcgacctgta cgacgcgggc
     1981 gtggccgtcg cgctggggca ggacgacatc gccgacgcct actacccctt cggccggaac
     2041 aacatgctgg agatagcctt cctcgccgtc cacctgctct ggatgaccac gttcagcgac
     2101 atggagatcc tctacgacct gatcaccacg aacgcggcga aggccatggg gatcaagggg
     2161 cacgtcctcg agcccggcgg aaacgccgat ctcgtcgtgc tcaacgcgga ggacgtctac
     2221 cacgcaatct gggagcacga cgctcccctc cacgtcatcc gcaagggcaa ggacgtcacg
     2281 ctgaagtagg cgccgcaaga gatcgcaaga aagcacgccg cccccggaga agctttccgg
     2341 gggcggcgcg ttgtcgatca tatcaatgta tatcaaggaa catccaaaag ggcatccggg
     2401 atgcaaaccg gctgctcctt accggccttc tcgcctcagt ccaactcgcc gtgaccgttt
     2461 tccttcgtta ttcccgagac aagcccgtcc aagtcgtagc gaccctccgg ttccggtatg
     2521 tgcagcaggc gcagcatctc tttctcgctc tccagaaccc gcgcggcgat gaactccgcg
     2581 aacgggcggt cgtcggttcg cgcacgctcc agcaggtcga tatactccct gcgcagaatc
     2641 ggaggtataa gggccggtag atagccgtcc tgtatcagcg cggcgttcat cagcaaccgg
     2701 gagatgcggc cgtttccgtc gatgaacgga tggatgaaga cgaaacgctt gtggagctgc
     2761 gcggcgaact cgaccggatg atagccgccg cgttcactgt tcgcccattc gaagagagat
     2821 gccatctccg agggaattcg gcccggttcc gtcaccggat agctcgaccc cgtgatcagg
     2881 acggcccggc ttcggtagct cccggccgcg gattcgtcga tgttgcggta gaacatccgg
     2941 tgcatggtcc gcgcgtcctc ttccgagatg ctcgcgcttt gaagcagaga gaacatgaag
     3001 tcgtacgcct ccgcgtggcc gatggcctcg aacgagtcac gaagaggctt gccgcccgca
     3061 gtgagcccgt cctccagcag caccttggtt tcgctctccg tgagggtatt cccctcgatg
     3121 gcgttgctcg accaggtcag gccgatgcgg tagtacctcc gtatctcgtc gagcatcgct
     3181 ccttcgaacg gacgcacccc gtcgatcgcc gctttcagcc ggtccactct ttggaagatc
     3241 gtcattgcgc atgcctccgt tcttttctcc ttctccgctt ctcacaccaa cgacttcgct
     3301 ccgctctttt tgcgctggac gaccttcctg ctgatcttca ggcgctccat gatgccgtcc
     3361 tgaagaatct gcgagtagtt caaggcggcg cgcttggcct gactgtccag ccacccgggc
     3421 agagtgaccg ttctgttcac ggccttcgtc tccatcctct cacggaagga cggcatccag
     3481 acttcgacca gcacggcgac gatcttttcg ccctcctcca cgtattcgcc caggtcgagc
     3541 cgatgcaagg gggtgggagc cgggatttca tctccgtcga cctccatgcc ccaaaggtgc
     3601 agcgacatgg cttctctcgc gtgctccagc gcttcgtcct cggttgctcc ggtcgtggtg
     3661 caaccgggaa ggtcgggcca gaggacataa tactgattcg ttgaagcatc gtaccccagt
     3721 acggcggggt aacggtatct gtctggcgtg ttcatgactc cgtctccttt cgggaagaag
     3781 gcggctgaag gagccccgct tgcttccaaa tcgacttcgc agtaggaccg gggacgcttt
     3841 tatcggggtg ctttacggtt accttgccct tttttgcggg atgcttgaac tgataatggc
     3901 tcccctccgc tccgtgcagc tgccatccgt cagcttcgag gatcttgatg att
//
LOCUS       DAOPKU010000263         3938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_25359_length_3938_cov_4.448622, whole genome shotgun sequence.
ACCESSION   DAOPKU010000263 DAOPKU010000000
VERSION     DAOPKU010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25359_length_3938_cov_4.448622"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..169
                     /locus_tag="PK849_08865"
     CDS             8..169
                     /locus_tag="PK849_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66276.1"
                     /translation="MTTMNPENRTLRRISVTECAAEAGEILDILMGDAVGPRRDFIFA
                     HATEADMDV"
     gene            196..1230
                     /locus_tag="PK849_08870"
     CDS             196..1230
                     /locus_tag="PK849_08870"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPE66277.1"
                     /translation="MTMHDARKRAEELHRENFVLDAHFDLLTFVLDKRQKGEKSVIEK
                     DYLPGLRAANVDVVISSLFVSDRFIPEMALRRALDMIAALHEEMAESPGLFSLCRNTA
                     EIAAANARGEIAFLLSFEGLDPIGNDLSLLRVFYELGVRGIGLVWSRRNYVADGCFFD
                     PVREGRKGGLTAFGVQVLDEAKRLGMYIDISHLNDEGVEDVFALYDGPVIASHSNCRA
                     LAPVMRNLTDDQVSRLAERGGVMGMNACSVFVADPDAKAVTERDLADHVDHIRSLVGD
                     GHVGFGFDFCDEIRDFAAPGPHREYDCIKGYGASVEFTAELLRRGYGEDAVRSIMGGS
                     FLAFLKQTIG"
     gene            1318..3141
                     /locus_tag="PK849_08875"
     CDS             1318..3141
                     /locus_tag="PK849_08875"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPE66278.1"
                     /translation="MSLFFVLLCAVCILFLLFPRASRACTSVVAGRNASSTGRVIVGH
                     NEDDPGRLIVRHALVPSRRWDDGDVITAEERSARIPQPPRTLAFFWTQVRGERGLSNA
                     DGFYNERGVLVVSDSCLSSRLDDEPDLVAGGIGYALRRAVTERASNARSGVEIAAQLV
                     DRYGYRSPGRGYVIADSKEAWIVQFAGGKDYCGVRVLDDEVVFFPNHFTIRAGDLEGR
                     EHILSPGLAEKALSRGWSAHEEEGLCSFDFAKAFQDPGSWKKDGNTYRHRHGVAAFRG
                     PLLAEEDDLPFAVLPERLITPEDVKAVLRSHYEGTPDDIRSDFAGRSPHFTSVRRICT
                     GGTVESMVALLSDDPLFTELLISRGRPCCDPYQPYWGGMTTLPKELSPIMEPRSAIDA
                     HCAAEPGLLDISDAAWWQLFHEQTLADLLYEHIHPALSEWIARREEELSAIAPKIAAE
                     ARVLAQSGETDAARRLLTEESAALAGASAVRKAEFLSAYGPRVLSPERAEIVKGDISG
                     ELTVFFQSQSAPVEDSFLMGQGGTPPQRWAAPLRGSLHFADGAWSLRFRVRELTEAAV
                     PCLADFWLAGRDEKGGVFAGRCLLKTLPPSEHQLFQGRCSL"
     gene            3206..>3938
                     /locus_tag="PK849_08880"
     CDS             3206..>3938
                     /locus_tag="PK849_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPE66279.1"
                     /translation="MALKKVWIHSLFAAVSILVFVSSGARAHDGDAELFRRGSVDDIR
                     AAIEAGADVNARDEEGIPVLTWCAQDNTAQAVALLLAAGADVNAESSRGWTPLMAAAS
                     GNPDPGVVSLLVKAGAEVNAKGEEDWDALMYGTWVDAPPSVIEALLKAGADVEASSVH
                     GLTALMLAAENSSTGESVALLLQAGAEANAADAQGATPLMYAAKSNPLVKIVELLLEA
                     GADPHLCDLNGNTSFSLAAEHNLNPE"
BASE COUNT          679 a         1195 c         1342 g          722 t
ORIGIN      
        1 gctctgggtg acgacgatga accccgagaa caggacgctt cgcaggatca gcgtcacgga
       61 gtgcgcggcc gaagcgggag agattcttga catactgatg ggcgacgcgg tcgggccgcg
      121 aagggacttc atcttcgcgc acgcgaccga ggccgatatg gacgtgtagc gcgagaaaac
      181 gaatggagga caataatgac aatgcatgat gcgcggaaac gcgctgaaga gcttcaccgg
      241 gagaacttcg tgctggatgc gcacttcgat ctgctcacct ttgttttgga taagcggcag
      301 aagggcgaga agagcgttat cgagaaggac tacctccccg gcttacgggc ggcgaatgtg
      361 gacgtcgtaa tctcgtcgct cttcgtctcg gaccggttca ttcctgagat ggcgctgcgg
      421 cgggcgctcg atatgatcgc cgcgctccac gaagagatgg cggagagccc cggtctcttc
      481 tcgctgtgcc ggaacactgc ggagatcgcg gcggcgaacg ccaggggaga gatcgcgttc
      541 ctgctttctt tcgaggggct ggacccgata ggcaacgatc tctcgctcct gcgggttttt
      601 tacgaactgg gagtcagggg catcgggctt gtctggagcc gcagaaacta cgtcgcggac
      661 ggctgtttct tcgaccccgt gagggagggg cggaagggcg gactcacggc gttcggtgtc
      721 caggtgctcg acgaggcgaa acgtctgggg atgtacatcg acataagcca cctcaacgac
      781 gagggcgtgg aggacgtctt tgccctctac gacgggccgg tgatcgcctc gcactcgaac
      841 tgccgcgcgc ttgctcccgt gatgcgcaac ctgacggacg accaggtttc caggctggcc
      901 gagcgcggag gggtgatggg gatgaacgcg tgcagcgtct tcgtcgccga tccggacgcg
      961 aaggccgtga cggagcggga tcttgccgac cacgtcgatc acattcgttc gctcgtcggc
     1021 gacgggcatg tcggcttcgg gttcgacttc tgcgacgaga tacgcgactt cgccgccccg
     1081 ggaccgcatc gcgagtacga ctgtatcaag gggtacggcg cgagcgtcga gttcactgcg
     1141 gagcttctgc gcagggggta cggcgaggac gccgtccgca gtattatggg aggttcgttc
     1201 ctcgctttct tgaagcaaac gatcggatag actaacctgg ccgtagcgaa ttcaggttgt
     1261 ttcgctttcg cctgaatgtc cgagctgccc ccggatcaac caaaggagct ggtgagcgtg
     1321 tcgttgtttt tcgttcttct gtgcgctgtg tgcatactgt ttcttctgtt tccccgagcc
     1381 tcccgcgcat gcacctccgt cgtcgccggg cgaaacgcct cctcgacggg gcgcgtcatc
     1441 gtcggccata acgaggacga tccggggcgc ctgattgtcc ggcacgcgct cgtcccctcc
     1501 cgtcgctggg acgacggcga cgtcatcacg gccgaagagc ggtcggccag aatccctcag
     1561 cctccccgga cccttgcttt cttctggacg caggtcaggg gggagcgggg cctttcgaac
     1621 gcggacggtt tctacaacga gcggggagtg cttgtcgtca gcgacagctg cctctcctcc
     1681 cggctggacg acgaaccgga cctcgtcgcg ggcggcatcg ggtacgcgct tcgccgtgcc
     1741 gtcaccgagc gcgcttcgaa cgcccgctcc ggggtggaga tcgcggcgca actcgtcgac
     1801 aggtatggct atcgcagtcc gggcagaggg tacgtgatcg cggattcgaa agaggcgtgg
     1861 atcgtccagt tcgcgggggg aaaggactac tgcggcgtgc gcgtgctcga cgacgaggtc
     1921 gtcttcttcc ccaatcactt caccatccgg gcgggcgacc tcgaaggccg cgagcacatc
     1981 ctgtcgccgg ggctggcgga gaaagccctc agtcgaggat ggagcgcgca cgaagaggag
     2041 gggctctgca gtttcgactt cgcaaaggcc ttccaggatc caggctcgtg gaagaaggac
     2101 ggcaatacct acaggcacag gcacggcgtc gcggcgttcc gcgggccgct gctcgcggag
     2161 gaggacgacc tccccttcgc tgtgcttcct gagaggctga tcaccccgga ggacgtcaag
     2221 gctgtgctcc gcagtcatta cgagggaacg ccggacgaca tccggagcga cttcgccgga
     2281 aggtcgccgc acttcacctc cgttcgccgc atctgtaccg gcggcacggt cgagagcatg
     2341 gtggcgctgc tctcggacga cccgctcttc accgaactcc tgatctcgcg cggacgtccc
     2401 tgctgcgatc cctatcagcc ctactgggga ggaatgacga cgctgccgaa ggagctttcc
     2461 ccgatcatgg aaccccgaag tgcgatcgac gcgcactgcg ccgccgagcc gggacttctc
     2521 gatatctcgg acgccgcgtg gtggcagctc tttcatgagc agacgctcgc ggatctgctc
     2581 tatgagcata tccacccagc cctttcggag tggatcgcga gacgcgaaga ggagctctcg
     2641 gcgatcgctc cgaagatcgc cgcggaggcg agggtgttgg cccaaagcgg cgaaacggat
     2701 gccgcccggc gtctgttgac ggaggaaagc gctgctttgg ccggggcgag cgccgtgcga
     2761 aaagcggagt ttctttccgc gtacgggcct cgcgtactca gtccggagcg cgcggagatc
     2821 gtcaaggggg atatctcagg tgagctgacc gtcttctttc aatcgcagtc tgccccggtc
     2881 gaagactcct tcctgatggg gcaggggggg acgcccccgc agcggtgggc cgctcccctg
     2941 aggggaagcc tccacttcgc cgacggcgcg tggtcgctcc gcttccgcgt gcgagagctc
     3001 accgaggcgg cggtcccgtg cctggcggac ttctggctcg ccgggcgcga cgagaagggc
     3061 ggcgtcttcg cgggccgctg tcttctgaaa acgcttcctc cgtcggagca tcagctcttc
     3121 cagggacggt gcagcctgta aacaaccgga gcggggtatg cggatgtccg tacagccccg
     3181 cttttttttc aaggagcgtg atggaatggc tctcaagaag gtgtggattc actctctctt
     3241 cgcggcggtt tccatcctcg tttttgtctc ttccggggcg cgtgcgcacg acggggatgc
     3301 cgagctgttc cgccgcggca gcgtcgacga catccgcgcg gccatcgagg cgggggcgga
     3361 cgtgaatgcg cgcgacgagg agggaattcc ggtattgaca tggtgcgctc aggacaatac
     3421 cgcgcaggcc gtcgcgctgc tgcttgcggc tggggctgac gtcaatgcgg agtcaagccg
     3481 cggatggact ccgctcatgg ctgccgcaag cgggaatccg gaccccgggg tcgtctcttt
     3541 gctcgtgaag gcgggagcgg aggtgaacgc gaagggcgag gaggactggg acgcgctgat
     3601 gtacgggaca tgggtcgacg cgcccccttc ggtgatcgag gcgctgctga aggcgggcgc
     3661 ggacgtagag gcttcgtccg ttcacggcct aaccgcgctg atgcttgccg ccgaaaacag
     3721 ctcgaccggg gagagcgtcg cgctgctgct ccaggccggc gcagaggcga acgccgcgga
     3781 cgcccaaggg gcgacgccgc tgatgtacgc cgcgaagagc aatccgctcg tcaaaatcgt
     3841 cgaactgctc ctcgaagcgg gcgccgatcc gcacctttgc gacctcaacg gaaacacttc
     3901 cttttccctg gcggcggaac acaacctcaa tcctgaag
//
LOCUS       DAOPKU010000264         3938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_25362_length_3938_cov_3.394798, whole genome shotgun sequence.
ACCESSION   DAOPKU010000264 DAOPKU010000000
VERSION     DAOPKU010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25362_length_3938_cov_3.394798"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..2801)
                     /locus_tag="PK849_08885"
     CDS             complement(270..2801)
                     /locus_tag="PK849_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPE66280.1"
                     /translation="MRQTRCAIFLFCILFSVLSTPYASSALVHDFDISYYRYLNTAIV
                     KNPDAAKIEEIIRYWADAIYEASNGLEHLGTVRIFQGPSSFLSAYRLSDIVWEKAGHP
                     KAAYGAYGSYDAQKPLGFRMAMFDGFKEYASGTAICDFLAPEHRMYAGYALASLSSYY
                     IHDLREEYAIERGGKKYNALAYPSILNDVSLAAYAGDLRALNHSTPRLYADAPSTVQK
                     AKWGISAWEYLTKRRAFPAQLAPVPPKNKRDASLNESEYRFLIQPGKHNAEAQSRLRI
                     VWVSSPVFQIVIDRSGSMEGERIVKAKNAAKDFVRSLQPGVRVGVIVFDDAVNVVSPI
                     VAIGSGDAEKKKLYSAIDGISLGGATAIYDACAVASKEMTADRNSYFEGRYVVLFTDG
                     EDNRSKIGVAPLIQALRKENVSVLVMGLEDEVREKTLREIASGTKGYYMMTADPLDTQ
                     EMLYGYLNSAFESQISTFDATLPAGGSTRTAFAVEEGTELLRVSLVSDGEISPKTLVF
                     SATPPKGKASAALHDKETGKLEILQKKPAAGKWTVTLKNNSKGTRRVRISAFSRTQSR
                     TLYLGNALTVRGGEANSAVIYASLTGNVTPHTGIGIKGTLRYPSGKNAPLEFQGMGYG
                     PDATQGDGVYSALAENCSEPGNYRVTVAFSNTGQAVETNQRYAFLPPPGIKDGEAYVV
                     AEKGHALPHKLERAFTTTFQIEGGKVTDDSRGRIEREKPIDFERVKSYLERGEMALKR
                     GAYHDAEDYAKSVLRMDPNNAAAYRLIGEAKDARNDFAGAVEPYAKAVELEPGEPSHY
                     FKLGQALANIRALDEAKKIYSIMQQAFPNNKWTNQLEMAIRFMSR"
     gene            3212..3851
                     /locus_tag="PK849_08890"
                     /pseudo
     CDS             3212..3851
                     /locus_tag="PK849_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT          863 a         1012 c         1120 g          943 t
ORIGIN      
        1 catcatggag gatcgcacgg tgttccggaa ggcgactcaa gcgatgttcg gaaggcctct
       61 cgaaggggtg cgctcaagga gagtctgcgt tttcgagaaa gaagacaaga aagggaagaa
      121 tccccccctc gtcttctttc tcgaataaac cgataccgac ggcttcggtt cgtgacgccg
      181 cgcggcgttc tttccaatcg accgacgaac gagagcattc caaggatcgg tggggcgcaa
      241 gggctctctc gacgcttttt tcctctccgc tatcgcgaca tgaagcgtat cgccatctcg
      301 agctggttcg tccacttgtt gttagggaaa gcctgctgca tgatcgaata gatcttcttc
      361 gcctcgtcga gggcgcggat gttcgccagc gcctgcccca gcttgaagta gtgcgaaggc
      421 tctcccggtt ccagttccac tgcttttgcg tacggctcca cggctccggc gaaatcgttg
      481 cgggcgtctt tggcctcgcc tatcaggcgg tacgcggcgg cgttgttcgg gtccatacga
      541 agcacgctct tcgcgtaatc ttcggcgtcg tggtatgccc cccgcttcag agccatttct
      601 cccctttcga ggtatgattt gaccctttca aagtctatcg gtttctctct ttcgattctc
      661 cctctggaat cgtcggttac ctttcccccc tctatctgga aggtcgtcgt gaaggcgcgt
      721 tcgagtttgt gaggcaaagc gtgtcctttt tcggccacga cgtacgcctc gccgtccttt
      781 atgccgggag gaggcagaaa ggcgtagcgc tggttggtct ccaccgcctg cccggtattg
      841 gaaaaagcga ctgtgactct gtaattcccc ggttccgagc agttctcagc gagagcgctg
      901 tagacgccgt ccccttgggt ggcgtcgggg ccgtagccca taccttgaaa ttcaagaggg
      961 gcgttctttc ccgaaggata ccgaagcgtc cccttgatgc cgattcccgt atgaggggtt
     1021 acatttcccg tgagggatgc gtagatgact gcggagttcg cttccccgcc tctcacggtg
     1081 agggcgttcc ccaggtagag cgttctggat tgcgttcgtg agaatgcgga gattcttacg
     1141 cgccgcgttc ctttcgagtt gttcttcagc gtgacggtcc atttcccggc agcgggtttc
     1201 ttctgaagga tctccagctt cccggtttcc ttatcgtgaa gcgcggcgga agctttgccc
     1261 ttcgggggag tcgcggagaa tacgagcgtc ttgggcgaga tctctccgtc ggaaacaaga
     1321 gagacccgca gcagttccgt gccttcctcc acggcgaacg ccgtccgcgt cgagccgcct
     1381 gcaggaagcg ttgcgtcgaa cgtggatatc tggctttcga aggcgctgtt gagatagccg
     1441 tagagcattt cctgggtgtc cagcggatca gccgtcatca tgtagtatcc tttggttcct
     1501 gatgcgatct cgcggagcgt cttctcgcgc acttcgtcct cgagtcccat gacgagcact
     1561 gaaacgttct ccttccgcag cgcttgaatc aggggagcga cgccgatttt agagcggttg
     1621 tcctctccgt cggtgaagag gacgacgtag cgtccctcga aatacgagtt tctgtccgca
     1681 gtcatctcct tcgacgcgac ggcgcaggcg tcgtagatcg cggttgctcc cccgagcgaa
     1741 atgccgtcga tagcggagta gagcttcttt ttttcggcat cccccgagcc gattgcgacg
     1801 atgggtgaga cgacgttcac agcgtcgtcg aagacgatca cgccgacccg cactccaggc
     1861 tgaagcgagc ggacgaagtc cttggcggcg tttttcgcct tgacgatccg ctctccttcc
     1921 atgctgccgg aacggtcgat gacaatctga aaaacggggg aggaaaccca gacgatacgc
     1981 aggcggcttt gcgcctccgc attgtgtttt cccggctgga tgagaaaacg gtattcgctc
     2041 tcgttcagag aagcgtccct cttgtttttt ggcggcacgg gggccaactg cgccggaaac
     2101 gcacgccgtt tcgtcagata ttcccacgct gaaattcccc acttcgcctt ttggacggtc
     2161 gaaggcgcat ccgcataaag gcgcggggtg gaatggttca atgcgcgcaa gtctcccgcg
     2221 tacgcggcaa gcgagacgtc gttgagtatg ctgggatagg cgagggcgtt gtacttcttc
     2281 ccgccccgtt cgatcgcgta ttcttcccta aggtcatgga tgtaataact tgagaggctt
     2341 gcaagcgcgt atcccgcgta catccggtgt tcgggagcaa gaaaatcgca gatggccgtt
     2401 cccgaagcat actctttgaa gccgtcgaac atggccatgc ggaatccgag gggtttctgc
     2461 gcgtcgtagg agccgtacgc gccgtaggcc gctttgggat ggccggcctt ctcccagacg
     2521 atgtcggaga gacggtaggc cgagagaaag cttgaaggcc cctgaaaaat gcgtacggtc
     2581 cccagatgct cgagcccgtt ggaggcctcg tagatcgcgt ccgcccagta gcggatgatt
     2641 tcttcgattt tggcggcgtc gggattcttg actatggctg tgttcaggta gcggtagtag
     2701 ctgatgtcga agtcgtgaac aagcgcggac gatgcgtatg gggtcgacag aacggagaaa
     2761 agaatacaga ataagaaaat agcacaacgt gtttgtcgca aagagaactc ctcctttggt
     2821 tttatggatt tttcagtagc ccctaatcct caggcagaaa aaaccctctg gactgttcgt
     2881 agaacagtga taaggtagtg tagacacagg ccttcaccaa aggggtgcat ctattatggc
     2941 aaagaacacg ggaaaaatcg atatcgagat gcacaaagct tccctctctt ggagatttct
     3001 tttccataat cgaactgttc atcaaaatgg gtctcgacaa agcgatggag gcgttgagag
     3061 gcgcgagaaa cgagcgtggc acgaaggact ctgagcgccg gggcagagag tggctcgatg
     3121 tagggactgg acagcggatt gaaggtgttg ttcgtgatat atttttactt agagtttttt
     3181 tcaaagctcg tttgacagga ggaatcaatt cgtggtcata aaaaataaat tgggtattga
     3241 tgatcagacc gaacttgctc gtgctgaaga aaaaacaagc aaggaaaaag cactggcatt
     3301 gtttgaatta aaacttttcg atactctcga agcgggaacc ttcaagggat tgtcagatat
     3361 tcatcgatat ttgttcgaag acatctacga ctttgcggga aaactgcgaa ccgagaacat
     3421 cgccaagggt aattttcgct tcgcccccgt aatgtacctc gaaacgtctc ttcaaaacat
     3481 cgaaaaaatg ccccaatcca ctttcgatga gattgttgaa aagtacgtgg agatgaatgc
     3541 ccgctcatcc gttcagagaa gggaacggca gaagcgctcg tatatggctt gacgtcatct
     3601 tgagaaaaga gttgaaactg gtgatcgatt ggagcatggt ggataaaaac gattaccttc
     3661 tcgccatgga aagaagccct atcaaggatg tcgaaatcaa ggcgcttctt tttgcggctt
     3721 tgacggataa aatcgacgac cgagacgtct acatgaaagg catcgacgcg agctatcatt
     3781 atgaaggata caacgtgttc aggacgaagg atttgaattc ccggcattgt gattacaaca
     3841 agcgaagttg attttcaaag agtcccgatc ctttctacat gcagagtaga gagaaggtgg
     3901 atattacgat ctccgagagc caccagtccg atctttac
//
LOCUS       DAOPKU010000265         3905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_25663_length_3905_cov_5.240000, whole genome shotgun sequence.
ACCESSION   DAOPKU010000265 DAOPKU010000000
VERSION     DAOPKU010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25663_length_3905_cov_5.240000"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            782..1069
                     /locus_tag="PK849_08895"
     CDS             782..1069
                     /locus_tag="PK849_08895"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66281.1"
                     /translation="MNEEEAEDERNGILSKLHDKTFCGLLPNDFCTVLLKEHPEYADV
                     FSAFRESGSLGWGITGSGSSAFGLFHQNDGFSRMIRSCRHMQWIRKILFVE"
     gene            1076..1876
                     /locus_tag="PK849_08900"
     CDS             1076..1876
                     /locus_tag="PK849_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPE66282.1"
                     /translation="MKGQRTERLIRTSARFLTCPSRQLSLTSLAEDFLVSKTVISDDV
                     AIIDEAFSREGLGGIIVDRGRTGGASFVPRIAPEIKDTLLNDLINLLNSEDRFLPGGL
                     VYYSDILFNPHYALRLGYAMASLFSETRPDIVMTSEVKGIPLAIFTAHAMGIPLAVCR
                     FRNRASDGSAVTVHYPTKNGEVKAMYMGTKQLTRGKRVLIVDDFMRGGSTAAGMLLVA
                     KEFGAEVAGTGIFIVSSDPEEKAVSSYKALLRLDGVEKRQPKVSLWEE"
     gene            1943..2125
                     /locus_tag="PK849_08905"
     CDS             1943..2125
                     /locus_tag="PK849_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPE66283.1"
                     /translation="MRVSIEKDECIGCGVCAQVCPEVFSMDDDAGVSKVIRPEGGECA
                     KEAADSCPVGCITVEE"
     gene            2206..2282
                     /locus_tag="PK849_08910"
     tRNA            2206..2282
                     /locus_tag="PK849_08910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2240..2242,aa:Ile,seq:cat)
     gene            2344..3123
                     /locus_tag="PK849_08915"
     CDS             2344..3123
                     /locus_tag="PK849_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPE66284.1"
                     /translation="MIVGELERRLDEAIPFIWAEGWDNVGLLLGDRSTLVRGTAVALD
                     PSIDAMKAALDHSCSVLITHHPLIFHPLKRIDCAEGPGAQIAFAIRHGLTVFSLHTNW
                     DVAPGGVNAAIAEVLSLQEVRPLLPSTTGAWGSGAAGKFPRPLNFLECGAAIRDALHL
                     SRLELHGDMKTSVSTLALCGGSGGTFLPEAFACGAEAYFTADMKYHERLDALDKGVSL
                     FIADHGEMERFSLTALAETVSKATGEKAKILDVSQPAFCMI"
     gene            3215..>3905
                     /gene="trpS"
                     /locus_tag="PK849_08920"
     CDS             3215..>3905
                     /gene="trpS"
                     /locus_tag="PK849_08920"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPE66285.1"
                     /translation="MAQRVFSGMRPTGKLHLGHLAGALTNWVKLQENYKCFYSIVDWH
                     ALMSEYADSSMIAENCKEVLLDWLASGLDPEKCVIFVQSHIPQHAELHLALSMVTPLG
                     WLQRCPTYKEQILNIQNKDLSNYAFLGYPVLMAADILLYKAEIVPVGEDQSAHLEITR
                     EMARRFNSFFGNILPEPQIMLTPTPKVPGTDGRKMSKSYGNAINIADTPSEMWNKIRP
                     MITDPARERRTD"
BASE COUNT          923 a          946 c         1070 g          966 t
ORIGIN      
        1 aaaccttgct gtcctgcgat tcgacgtaaa gagtaaagag attcggatag gtttccttca
       61 gttatgcgga gcttttgact cgcgaagtcg agttggagct cttgtccgga aacagaaatt
      121 aagtctctgc tgcttatgtt aaaagtatgc cccgccacgt cgcccgtgga ggggcatact
      181 ttttgttaat tattcaagga aggagctgct ccttttgatt cactgtctgc aatccgcggc
      241 gaaggtgaac atcacgcttc gagtcaccag aaaacgtgat gacggttatc atgaacttcg
      301 ttctcttttt tatgcgcttc ccgcaattga atcattgaca attgaacctg attatggtca
      361 taatgtaaag gacataatct ccgtaacagg agaaatgatt cgcggaagaa acatccttga
      421 agaagttctt tctgcggcgc gaaaaaaaag aggagtggat cctctgcgca tacggcttca
      481 taaggtcata ccccctggga gcggcctcgg cgggggaagc ggaaacgccg cagctctgat
      541 ttcttggctg aactctcagc gtgaagagaa tgaaggaatt cgaggcgaag agattgggtc
      601 cgacgttccg ttttttttga acgcagagaa atgggctttc gtcggaggca gaggtgaacg
      661 tgtggaacct ctctccggga ttatgccgcc ctttcacgca gtcgttgtca ttccctcatg
      721 gagtatgtcg acggcaagag ccttttctct tctcacagcc gaatacggcg gttttttccc
      781 catgaacgag gaggaagccg aagatgagag gaatggtatt ctctcaaaat tgcatgataa
      841 aacgttttgc gggcttctgc cgaatgattt ctgcacagtt cttctgaaag agcacccgga
      901 atacgccgac gtttttagcg cattccggga aagcggatcg cttggatggg gtatcacagg
      961 aagcggcagc tcggctttcg ggctgtttca tcaaaatgac ggtttttcgc gaatgattcg
     1021 gagctgcagg catatgcaat ggatacgcaa aatcttattt gtggagtgat gatgaatgaa
     1081 aggacaacga accgaacgat tgatccgtac ttcggcacga tttctcacat gcccttcgag
     1141 acagctctcg ctgacatctc tggccgagga ttttcttgtc tcaaagactg tcatcagcga
     1201 tgacgtcgcg atcattgacg aggctttttc aagggaaggc ctgggcggca ttatcgtcga
     1261 cagggggaga accggaggag cttcatttgt accgcgcata gctcccgaaa tcaaggatac
     1321 gctcctgaac gacctcatta atctgttgaa cagcgaggat cgtttcctgc ccggcgggct
     1381 tgtgtattac agcgatatac tctttaatcc gcactacgct cttcgtctcg gctatgcgat
     1441 ggcctcgctc ttcagtgaaa ctcgtccgga cattgtcatg acatccgaag taaagggcat
     1501 tccgctcgct atttttactg cccatgcgat gggcatcccg cttgcggtct gccggtttcg
     1561 taacagggca agcgacgggt cggcggtgac agttcactac ccgacaaaga acggcgaggt
     1621 caaggctatg tacatgggaa cgaaacaatt aacccgcggg aagagagtgc tcattgtcga
     1681 cgactttatg cgcggcggaa gcactgcggc cgggatgctc ctcgtcgcaa aggaattcgg
     1741 cgccgaagta gcagggactg gaatattcat cgtctcttcc gaccctgagg aaaaagcggt
     1801 ctcttcgtac aaggcgctcc tcaggcttga cggcgttgag aagagacagc cgaaagtttc
     1861 cttgtgggaa gagtaaggga tttgacgata tctttccgct gatatacaat cccgtagacg
     1921 gaaggaggtg accacatctt ccatgcgtgt ttccattgag aaagacgaat gtattggatg
     1981 cggcgtgtgt gcgcaggtgt gtccggaggt attttccatg gacgatgatg caggcgtgtc
     2041 caaggtaata cggccggaag gcggcgagtg tgcaaaagaa gccgccgaca gctgtccggt
     2101 tgggtgtatt accgtagagg agtgacaagg cccacttttt cgaaaaagcg gtatgcgctt
     2161 gctttttctt gttcctcggg cgtataatga gcaacgcttc atgaagggcc tatagctcaa
     2221 gtggttagag ccaccggctc ataaccggaa ggttcctggt tcgagtccag gtaggcccac
     2281 cacgtacatt gccgtttcct tccggggaaa cggttttttt atttttctgg aaggtgatgc
     2341 tctatgatcg ttggagaact tgaacgcagg ctagacgaag cgattccttt catatgggct
     2401 gaaggatggg acaatgtcgg tcttctcctg ggagaccgtt cgaccctcgt tcgcggcacg
     2461 gccgttgctc ttgatccgtc gatcgatgcc atgaaggcgg cgctcgatca ctcttgctcc
     2521 gtgttgatca cacaccaccc gctcatcttt cacccgctga aacggataga ctgcgcagag
     2581 gggcccggcg cgcagatcgc tttcgccata cgccacggct tgacggtatt ttcgcttcac
     2641 acgaactggg atgttgctcc cggcggagtg aatgccgcta ttgcagaggt tctctctctg
     2701 caggaagtcc ggccgcttct tccttcaaca acgggcgcct gggggagcgg ggcagccgga
     2761 aagtttccac gacctctgaa ctttctggag tgcggagctg caatacggga tgcgctgcat
     2821 ctctccaggc ttgaactcca tggcgatatg aaaacaagcg tgtcgacgct ggctctgtgc
     2881 ggaggctccg gcggaacgtt cttgccggag gcgttcgcat gcggcgctga agcgtatttc
     2941 acagcggata tgaaatacca tgagcgtctt gacgctcttg acaaaggagt ctctcttttc
     3001 attgccgatc acggagaaat ggagcgtttt tctctcacag cgctcgcgga gacagtttca
     3061 aaagcaacgg gcgaaaaggc aaaaatcctg gatgtttcac agcctgcatt ttgtatgata
     3121 tagttctttt ggtgctgaag actgagtacc gaatcatgat gattcttgat aaaccttgca
     3181 cgcagaggat acttccattc gaggtggttt gattatggca cagcgtgttt tcagtggaat
     3241 gagacctaca ggaaagctcc atctcgggca tcttgcagga gcgttgacga attgggtaaa
     3301 gcttcaggag aactataaat gtttttactc catcgtcgac tggcatgccc tcatgtccga
     3361 gtacgcggac agctctatga tagcggagaa ctgcaaagag gttttgctcg actggcttgc
     3421 atccgggctc gatcctgaaa agtgcgtaat ttttgtccag tcgcatattc cccagcatgc
     3481 agagcttcat cttgctcttt caatggtaac gccgctcgga tggctccaga gatgcccgac
     3541 gtataaggag cagattctca atatccaaaa taaggatctg agcaactatg ctttcttagg
     3601 gtatcctgtc ctcatggcgg cggatattct cctgtacaaa gcggaaatag ttccagtcgg
     3661 agaagatcag agcgcgcatc tggaaattac gcgggagatg gcccggagat tcaactcctt
     3721 tttcggcaat atccttcctg aaccgcaaat catgttgacc ccgacgccaa aggttcccgg
     3781 aacggacgga cgaaagatga gcaaatccta cgggaacgcc atcaacattg ccgatacgcc
     3841 ttcagagatg tggaacaaga ttcgccctat gatcacagat cctgcaagag aacggcgcac
     3901 cgacc
//
LOCUS       DAOPKU010000266         3896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_25757_length_3896_cov_4.599323, whole genome shotgun sequence.
ACCESSION   DAOPKU010000266 DAOPKU010000000
VERSION     DAOPKU010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25757_length_3896_cov_4.599323"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PK849_08925"
     CDS             complement(<1..412)
                     /locus_tag="PK849_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE66286.1"
                     /translation="MNSNTCLRVHSFFPRRLLRMGLLLLTGVLLLPLLVTPLFAERDT
                     MEYDVVVAGGGAGGTSAAIAAVRLGARVALLEETDWLGGQMTAAGVSTMDDLSGNKTG
                     IYGEFSENVRYHYFKKEKSVSTCYWDGNTTAFEPS"
     gene            527..820
                     /locus_tag="PK849_08930"
     CDS             527..820
                     /locus_tag="PK849_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66287.1"
                     /translation="MIYTVRFFGVLAMVLGVQSGAPISLELPEGSEYGSALDAIHEKY
                     GDRFPPSMWDRGKKMFSSGVLAFGDDGRFLPRTRSASLPKCGEIRFHLPLSGG"
     gene            complement(817..2421)
                     /locus_tag="PK849_08935"
     CDS             complement(817..2421)
                     /locus_tag="PK849_08935"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE66288.1"
                     /translation="MSKSVFYNGKIAVPGGFVEGMAIEGQNIVCLGSSEEVCAIRDAE
                     KIDLAKRLALPGFIDGHMHFLAYALSLERADLLGCFSVNEVRSRLRAFLDERRPEPGE
                     WVVGRGWNHELFDDNRILTKEDLDDLTPDNPAMLARVCGHIVVLNSRAITLLGLTGES
                     RFAGGVVDLDDGGEPTGVIRESVVEWAGSKLPLPGPEKLRKLVVRAGKETAKVGLTSI
                     HSDDLGSVGGDFQTIIDLYISLCKEDEMPVRITEQLLLRNRPALDHFLSTGWRTVDGM
                     PFFHIGPLKILTDGSMGGRTALLRKEYEDAPGVFGVPIYGHDELDDLVLTAHLAGMQV
                     ATHAIGDGALNMCLDAMERALLAKPRDARHYIVHCQMGDMEQYRRMARLGIGAAIQPP
                     FVPSDRPMALKRIGEERALAGYAWKTMKELGIFLSGGSDSPVEKFDPLWGVYTAVARK
                     DENGLPQGGWNPSQKFSVAEAVDLYTRGGAYASFEEHRKGTLAEGMLADVVVLDRDIF
                     SVPEDEIKEAHVALTMVGGRIVYRTD"
     gene            complement(2418..2684)
                     /locus_tag="PK849_08940"
     CDS             complement(2418..2684)
                     /locus_tag="PK849_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66289.1"
                     /translation="MRISGFSEDEDGNGCYLVEWADTAGRKFAVLYSESGGSVESVSA
                     ERKRELFENGDLEACSFPASEVFFPDEVQKLAERFQIVVEEEEE"
     gene            2778..3224
                     /locus_tag="PK849_08945"
     CDS             2778..3224
                     /locus_tag="PK849_08945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPE66290.1"
                     /translation="MEQLPRSKGCFVCGNSDDNPRSLGLTIFWNDEEQRTEIPILPNS
                     TWCGYEGVVHGGIIASVFDDAMAWAIRRESGSWAVTGELSVRYLRPVLAGTRYVVEGR
                     VEKSSGRRISTVAVMKEKNGKKVAEANALFVRIRERTISEGEEQTE"
     gene            3428..3886
                     /locus_tag="PK849_08950"
     CDS             3428..3886
                     /locus_tag="PK849_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE66291.1"
                     /translation="MSENIREESLALSERILALRRDLHRHPELSYKEFRTAGIVAGLL
                     RELGAEVWENVGRTGVVGVFRGGDGPVVAIRADIDALPVLEQTGLPFASETEGVMHAC
                     GHDIHTACACGAAMLLAKHVGSLGGSVKFIFQPAEEINDGAKAMLADGAM"
BASE COUNT          881 a         1130 c         1057 g          828 t
ORIGIN      
        1 ctgatggctc gaaggcagtc gtgttgccgt cccagtaaca tgtcgacacg gacttttcct
       61 tcttgaaata gtgatagcgc acattttcgc tgaattcgcc gtagatgccg gttttattgc
      121 cgctgagatc gtccattgta gaaacgccgg cggcagtcat ctggccgcca agccagtcgg
      181 tctcctcaag cagggcgacg cgggcgccaa ggcggaccgc cgcaatagcg gcgcttgtcc
      241 ctcccgcccc gcctcccgcc acaacgacat cgtactccat ggtatcccgc tctgcaaaaa
      301 ggggcgttac taacagaggc agaagtaaaa cccctgtcaa caaaagcagc cccatccgca
      361 gtaaacggcg tgggaaaaaa gaatgaacgc gcaggcatgt gttcgaattc atcgtcgatt
      421 ctcctcggtt aaaatttata tgttcgaaaa gtattataca actattcttc ctgtgcaacc
      481 ccgaaaggag tacggtacaa tatcgttgtc agaaaggagg ggttcgatga tttatactgt
      541 acgcttcttc ggcgtcctcg caatggttct cggcgttcag tccggagcgc caatctcact
      601 ggaactgcct gaagggagcg agtacggctc cgctctcgac gccatccatg aaaagtacgg
      661 cgaccgtttt cccccgtcaa tgtgggacag ggggaaaaag atgttttctt cgggagtgct
      721 cgcttttggc gacgatgggc gttttttgcc ccgcacccgc tccgcctctc tgccgaagtg
      781 cggagagatt cgttttcacc tccccctgtc cggaggttag tcagtccggt agacaatacg
      841 ccctccgacc atcgtgaggg caacatgcgc ttcttttatc tcatcctccg gaacggagaa
      901 gatgtctctg tcgagaacaa cgacatccgc aagcatccct tcggccagcg ttcccttgcg
      961 atgctcctcg aatgaggcgt atgcgccgcc tcgcgtatac agatcgaccg cctcggcgac
     1021 ggagaacttc tgcgacggat tccatccgcc ttgaggcagg ccgttctcgt cctttcgagc
     1081 gaccgcggta tagacgcccc acagcggatc gaacttctcg acgggagaat ccgaaccgcc
     1141 cgagagaaag attccaagct ccttcatcgt cttccacgca tagcctgcga gcgcccgctc
     1201 ctcgccgata cgcttcaacg ccatcgggcg atccgagggg acgaacgggg gctggatagc
     1261 ggcgccgatt ccaagccgcg ccatgcgccg gtactgctcc atatccccca tctggcaatg
     1321 cacaatataa tgacgcgcgt cgcggggttt tgccaggagc gcccgttcca tggcgtcaag
     1381 gcacatattg agcgcgccgt cgccgatagc gtgcgtcgcc acctgcatac ccgccagatg
     1441 cgccgtcaga acgagatcgt ccagttcgtc gtgcccgtag atagggacgc cgaaaactcc
     1501 cggagcgtcc tcgtactctt ttcgcaaaag cgcggttctc ccgcccatcg agccgtcggt
     1561 aagaatcttc agcgggccga tatggaaaaa aggcataccg tcgactgtcc gccagcctgt
     1621 cgaaaggaaa tgatccagcg ccgggcggtt ccggagcagc agttgctcgg taatcctcac
     1681 cggcatttca tcctctttac agagagagat gtagagatcg ataatcgtct gaaaatcacc
     1741 gccgacgctg ccaagatcgt ccgaatgaat cgaggtaagc ccgactttag cagtctcttt
     1801 tcctgcacgc actacaagtt tccgcagctt ttcaggcccc ggaagaggga gttttgagcc
     1861 cgcccactcc acgaccgatt cgcggatgac gcctgtaggt tcgcctccgt cgtcgaggtc
     1921 aacaactcct ccggcaaacc tgctctcgcc cgtaagccca agaagcgtta tcgccctgga
     1981 gttgagcaca acgatatgac cgcaaaccct ggcaagcata gcggggttat cgggcgtcag
     2041 gtcgtcaaga tcttcctttg taagaatccg gttgtcatcg aaaagttcat ggttccagcc
     2101 gcgccctaca acccattcgc cgggttccgg ccttctctcg tcgagaaagg ctcgcagacg
     2161 cgaacgcact tcattcacag aaaaacaccc gagcaagtcg gctcgttcga gagaaagcgc
     2221 atatgcgagg aaatgcatgt gcccgtctat gaaaccgggg agagcgagcc ttttggcaag
     2281 atcaatcttt tcggcatccc gtatcgcgca cacttcttcc gaacttccca gacaaacgat
     2341 attctgtcct tctatcgcca ttccctcgac gaatcctccc ggaacggcaa tcttcccgtt
     2401 gtagaacacg cttttgctca ctcttcctcc tcctctacaa caatttgaaa gcgctcggca
     2461 agcttttgaa cttcatccgg gaagaacacc tccgacgcag gaaaagagca ggcttcaaga
     2521 tcgccgtttt cgaaaagctc tcttttccgc tcggcggaga cgctttcaac gcttcctccc
     2581 gattccgaat acagaacggc gaacttacgt cccgccgtat cagcccactc gacaagatag
     2641 cagccgttgc cgtcttcatc ctcggaaaaa cctgaaatcc gcattcggat cgacctccaa
     2701 atttctttgg ctccattata cccttcttct cttcttcggc tataatcgtg cgaaacattt
     2761 gtgagaggag cggtacgatg gaacagcttc cacggtcgaa aggatgcttc gtctgcggga
     2821 attcggacga caaccccagg agcctcggtc tgaccatatt ctggaacgac gaagaacaga
     2881 ggacggaaat cccgatcctc ccgaacagca cgtggtgcgg ttatgaaggc gttgtccacg
     2941 gaggaatcat cgcctccgtc ttcgacgatg cgatggcatg ggcgatccgc agggagagcg
     3001 gctcgtgggc cgttaccggg gagctatcgg tccgctacct ccgtcccgtc cttgcgggca
     3061 cacgctacgt cgttgaaggg cgggtggaaa aatcgtcagg acgacgcatt tcaaccgtcg
     3121 ccgtcatgaa ggagaaaaac ggcaagaaag tggcggaggc gaacgccctc ttcgtgagaa
     3181 tacgggagag gaccatttcc gagggagaag agcaaacgga atgaatgctc atcgtgattg
     3241 cgccgctcgc ggcattacaa cagcgatatg atatactgtc ttttgtttga ttttcgtggg
     3301 aaggggcaat tattgttcag aaatctctgt acgcgaaaaa tccggtctga acagcttcaa
     3361 attgagcgtt ctaaagatgc cggctatctc agtaccggga gattatcgta agggagcgtg
     3421 ggaatgggtg agtgaaaata tcagggaaga gagccttgcg ctctcggaaa gaattttggc
     3481 gttgcgtcgg gacctgcacc gtcatccgga actgagttac aaagagttcc gcactgcggg
     3541 catcgtcgcc ggactcctta gagagcttgg agcggaggtc tgggagaatg tgggccgaac
     3601 cggcgtcgtt ggagtattcc gaggaggaga cgggcctgtc gtggcgatcc gcgcggacat
     3661 tgacgcgctg cccgttcttg aacaaacggg ccttccgttc gcttcggaga ccgagggcgt
     3721 aatgcacgcc tgcggacatg acattcacac cgcatgcgca tgcggcgcgg ccatgctgct
     3781 cgcaaaacac gtcggttcgc tcggaggaag cgtgaagttc atcttccagc ccgcagagga
     3841 aatcaatgac ggcgcgaagg caatgctggc ggacggagct atgtaaaatc cgaagc
//
LOCUS       DAOPKU010000267         3884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_25847_length_3884_cov_4.753460, whole genome shotgun sequence.
ACCESSION   DAOPKU010000267 DAOPKU010000000
VERSION     DAOPKU010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25847_length_3884_cov_4.753460"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /gene="trmB"
                     /locus_tag="PK849_08955"
     CDS             complement(<1..949)
                     /gene="trmB"
                     /locus_tag="PK849_08955"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPE66292.1"
                     /translation="MPEKQQLPLNILRARGETRVFLEIGFGNGEFLVHLARQNPDAVF
                     WGVEMSRSCVLRALKRIRREELRNIFLLCGDARFILKECLPKESLDGIYMNFPCPWPK
                     KKHTKRRVSSGDFPSEITSVLKKGAFFELVTDEEWYGMEVRNVLAAHPLLELTEWKIN
                     PPRPVTTKYERKWMEMGKRIFLSRLQKKEAESSVGYRCLGRTEDVHIRVPGKGSLKKF
                     VNDIHNMGGEKGDALWVYKRYYESAEGNAYIIETVAGDGSFEQKFFIQIVEREEDALI
                     KIAPYSSPFLTDSVRGALEGMAHLIETHYLSIASSCAASL"
     gene            1378..>3884
                     /gene="nifJ"
                     /locus_tag="PK849_08960"
     CDS             1378..>3884
                     /gene="nifJ"
                     /locus_tag="PK849_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPE66293.1"
                     /translation="MKRNWKTMDGNTAAAHVSYAFTEVAAIYPITPSSNMPEVVDEWA
                     AHGRKNIFGKTVRVTELQSEAGAAGAVHGSLSAGSLSTTFTASQGLLLMMPNMYKIAG
                     ELLPGVFDVTARSVAAHALSIFGDHSDVMACRGTGFAFLASGSVQEVMDLTAVSHLSA
                     IKGSLPFVNFFDGFRTSHEIQKIDVLEYDDLAKLVDYDALAKFKARAMNPEHPYQKGT
                     AQNPDIFFQAKEAVNRFYTVIPDIVESYMQEIKKLTGREYHPFNYYGDPNAENIIIAM
                     GSVCQAIEETVDYLNARGEKTGVVEVHLYRPFSEKYFFDVLPASVKRIAVLDRCKEPG
                     ALGEPLFEDICAIFKDKAEKPIVVGGRYGLGSKDTTPTHIKSVFDNLKLYQPRDRFTI
                     SIVDDVTGTSLPVNEQIVAAPEGTIRCKFWGLGSDGTVGANKNSIKIIGDETDLYAQG
                     YFAYDSKKSGGITVSHLRFGKKPIKSTYLIDTADFIACHKQEYVNLYDVLGGLKKGGS
                     FLLNTQWDTIEKLEKHLPSRMRRALANNDIQFYILNGTDIGEELGLGNRINMIMQAAF
                     FKLTNVIPINDAVKYMKDAIDHTYSRKGEAILEMNYKAVDAGINAIKKIDVPAEWANA
                     TSEPVLLSGLPSEIPDFVKDVCMPINAQQGDSLPVSAFLGREDGKFPSGTAAFEKRGV
                     AVNVPEWKSDKCIQCNQCSMVCPHAAIRPFILNEQELAAAPQGFGAVEVKGKEFPGCK
                     YKMQVDTLDCMGCGNCADICPVGALEMKPIVSQEKEIPNWLYAVEEVSDKSAETNKFT
                     VKGSQFSQPLLEFSGACAGCGETPYIKLVTQLFGDRM"
BASE COUNT          984 a         1026 c          950 g          924 t
ORIGIN      
        1 caagtgaagc ggcgcaggag gaggcgatcg atagatagtg cgtctcaatc aaatgagcca
       61 taccttcgag agccccgcgt actgaatcag tcaaaaacgg gctcgaatac ggcgctattt
      121 ttatgagggc gtcctcttcg cgctctacga tctggatgaa aaatttttgt tcgaaggagc
      181 cgtcgccggc cactgtttcg ataatataag cgtttccttc cgcagattcg taataccttt
      241 tatacaccca gagagcgtcg cccttttctc ctcccatgtt atgtatatcg ttcacgaatt
      301 tttttaaaga tccctttccc ggaactcgaa tatgcacgtc ctccgtcctc cctagacagc
      361 ggtatccgac agatgattcc gcttcttttt tctgcagtct ggaaagaaaa atacgtttcc
      421 ccatctccat ccatttgcgt tcgtattttg ttgtaaccgg cctgggcgga tttatcttcc
      481 actccgtaag ttcaagcaaa ggatgagcgg ccagcacatt tcgcacttcc ataccgtacc
      541 attcctcgtc ggttacaagt tcaaagaacg cccccttctt gagaacgctc gtaatctccg
      601 agggaaaatc tccgctggaa actcttcttt tagtgtgttt tttcttaggc caaggacagg
      661 gaaaattcat gtaaatgccg tcgagagatt cttttggaag gcattctttc agaatgaaac
      721 gcgcatcgcc gcacaggagg aagatgtttc gcagttcttc tctgcgaatt cttttcagag
      781 cgcgcagaac gcacgaccgg gacatctcga cgccccaaaa aacggcatcc gggttttgcc
      841 gtgcaagatg aacgagaaat tcaccgtttc cgaaacctat ttcaagaaat acccgcgttt
      901 cccctcttgc tctcagtata ttcagaggaa gctgttgctt ttcaggcatg ataataattt
      961 ctcgtatgtc gatcacaatt ccaacctctc cttgagcaaa ctcgtaccgg aatgcatacg
     1021 attatagcag ccttcggaac gaaatcggca aaagtgaagc gtttggagtg aaatgaacga
     1081 attgacccgt agaaggttta tggtaatatc aatacgtgaa aaaaatactt tttggttgtc
     1141 gtttttttcc agaagtgcgg attatacaga atgcaaaatc ccgatcttgt tgtaaaatag
     1201 aagggtgtta accggaagtc gaattcccat ctgaccttcg gcgtatttgt gcggacatta
     1261 caagagcccg ctggaaagat cacaatgggg cttattgcgt ctatggatca agaataataa
     1321 aacaggagcg ggttgcgaca acgctgcccc tcctgtaatt caagaggggg tacatcaatg
     1381 aagagaaatt ggaaaacgat ggacggcaac actgctgcag cgcatgtttc atatgcgttc
     1441 accgaggttg ctgcaattta cccgattacg ccgtcctcca atatgcccga agttgtcgat
     1501 gaatgggctg cccacggcag gaagaatatt ttcggcaaga ctgtcagggt cacggaactt
     1561 cagtctgaag cgggagccgc aggcgccgtg cacggttctc tttcagccgg atcgctttcg
     1621 accaccttca cagcatctca gggtttgttg cttatgatgc cgaatatgta caaaatcgcc
     1681 ggcgaactac ttcccggtgt ctttgacgtg actgcccgct cagtcgccgc gcatgccctt
     1741 tccatcttcg gcgaccacag cgacgtcatg gcttgccgcg gaacgggttt tgcatttctc
     1801 gcatccggca gcgtccagga agtaatggat ctgaccgctg tctcccacct ctccgcaatc
     1861 aaggggagtc ttccttttgt caacttcttc gacggcttca gaacctctca cgaaattcag
     1921 aagatcgacg tgcttgagta cgacgatctt gccaaacttg tcgactatga cgcgcttgca
     1981 aaattcaagg cgcgcgcaat gaatccggaa cacccctacc agaagggaac cgcgcagaac
     2041 cccgacatct tcttccaggc gaaagaagcg gtcaacagat tctatactgt tatcccggac
     2101 atcgtcgagt cgtacatgca ggagatcaag aagctcacag gcagagagta tcacccgttc
     2161 aactactacg gcgacccgaa cgctgaaaac atcatcatcg caatgggttc tgtctgtcag
     2221 gcgattgagg aaacggtcga ctacctcaac gcgcgcggcg agaagacagg cgttgtagaa
     2281 gtacatctgt atcgcccctt ctccgagaaa tacttcttcg acgtgcttcc cgcatccgta
     2341 aagcgcattg ctgtgctcga cagatgcaag gagcccggcg ctctcggcga gcccctcttt
     2401 gaggatattt gcgctatttt caaggacaaa gcggaaaaac cgatcgttgt tggcggacgc
     2461 tacggtctcg gttccaagga caccacaccg acacatatta aatctgtctt cgacaatctg
     2521 aagctttatc agcccaggga tcgtttcacc atcagcatcg tcgacgacgt taccggcaca
     2581 tcactcccgg taaacgagca aattgtcgca gctcctgaag gaacaatcag atgcaaattc
     2641 tggggccttg ggtccgatgg aactgtgggc gccaacaaga actcgattaa aatcatcggc
     2701 gacgaaacag acctttacgc acaagggtat tttgcgtacg attccaaaaa atccggcggg
     2761 attaccgttt cccaccttcg ttttggcaaa aaaccgatca agtccaccta tctcattgat
     2821 actgccgact tcatcgcttg ccacaagcag gagtatgtca atctctacga tgttctcggc
     2881 ggcctgaaaa aaggcggttc gttccttctg aacacccagt gggacactat cgaaaagctt
     2941 gagaagcatc ttccttcaag gatgagacgc gcccttgcaa acaacgatat tcagttttat
     3001 atcctcaatg gaacggacat cggcgaggag ctcggtcttg gcaacaggat caacatgatc
     3061 atgcaggccg cattcttcaa gctgaccaat gtcattccta taaacgacgc cgtgaaatat
     3121 atgaaggacg ccatcgacca cacctacagc aggaagggcg aggccattct cgagatgaac
     3181 tacaaggcag tggatgcagg catcaatgcg atcaagaaga tcgacgtgcc tgctgagtgg
     3241 gcgaacgcga cgtccgaacc ggtgcttctt tccggtctgc cgtcggagat tcccgatttt
     3301 gtcaaggatg tctgcatgcc catcaatgca cagcagggcg attctcttcc tgtcagcgca
     3361 ttcctcggac gcgaagacgg caaattccct tcgggaaccg ccgctttcga gaaacgtggc
     3421 gttgcggtaa atgttccgga atggaaaagc gacaagtgca tccagtgcaa ccagtgctcg
     3481 atggtctgtc cgcacgctgc cattcgcccg tttattctga acgagcagga actcgcggcc
     3541 gcgcctcaag gcttcggcgc agtcgaagtc aaaggaaaag agttccccgg ctgcaagtac
     3601 aagatgcagg ttgacacgct tgactgcatg ggctgcggca actgcgccga tatctgccca
     3661 gtgggcgcgc tcgaaatgaa accgatcgtt tctcaggaaa aagagatacc gaactggctc
     3721 tatgccgtag aagaggtttc cgacaagtct gcggagacga acaagtttac ggtcaaggga
     3781 agccagtttt cccagccgct cctcgagttc tcaggagcgt gcgccgggtg cggcgagacg
     3841 ccgtacatta agctcgttac acagctcttc ggcgaccgga tgat
//
LOCUS       DAOPKU010000268         3879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_25890_length_3879_cov_5.628138, whole genome shotgun sequence.
ACCESSION   DAOPKU010000268 DAOPKU010000000
VERSION     DAOPKU010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25890_length_3879_cov_5.628138"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK849_08965"
     CDS             <1..543
                     /locus_tag="PK849_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66294.1"
                     /translation="EDMMRNVEAGIRGFLVYDEGALKLVNQMRAEGFLPKETIFKWSV
                     FGGQCSPAGAKLLRELGADTMNPISDVSLPILSSIRSTVDMPLDVYMIIVDSFGGMFR
                     AYDAPEIVRVASPVYFKIEPGTSEGDIYKPWVTDSWHDEFIRAKVKIAATLREIMERR
                     APELKLSEQGPADLRLPVVD"
     gene            637..1203
                     /locus_tag="PK849_08970"
     CDS             637..1203
                     /locus_tag="PK849_08970"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE66295.1"
                     /translation="MKKLELKGLEKIDAVLTWVEERILVFSILFMAVILIANVISRNF
                     FNSSINASEEISQFLVVLATFLGTSYAARKGLHIRMSILNDFLKGKPQKMLALFVSLA
                     TAVIMFYISWLAFRYVRRVGTLHRVSPILQVPLHYIWSVVPVGFLLTGVQYLLTFARN
                     LTSGEVWVSYYVKDENAEPEAAPAEGGN"
     gene            1205..2485
                     /locus_tag="PK849_08975"
     CDS             1205..2485
                     /locus_tag="PK849_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE66296.1"
                     /translation="MLLMMIGVMCFFLVLGFPMIIPLIIAPIAVAAVYYPALDPMNLV
                     QQMLVGVRPLSLVAVPMFIFAADVISTGQVARRLIDFVMAFFGHTRGGLAVTTTAACT
                     FFGAVSGSTQATVVAIGGTMRPYLLRDGYDDSFSIALIINAAGIALLIPPSISMIIYG
                     VVTGSSVGELFIAGIGPGLLVFLLFSLYCSFAARKMDIVRTPKMPLPERVKALRKAIV
                     PIGFPLIILGGIYSGKFSPTEAAAVAVLYAVVVEIFLYRHIGWRDLYRIALSTGIVTA
                     VVFILVAVGASFSWLVGYARIPEAILPPILGESPSMIKLLLVIAASYFIGCMFVDSIV
                     VIMILAPIFHPIATKLGIDPILIGVLVTMQAAIGAVTPPFGCNIFTAMAVFRRPYADV
                     VRRIMPFLALYILATLIVIFFPSLSLFLRDMAFR"
     gene            2665..3696
                     /gene="dctP"
                     /locus_tag="PK849_08980"
     CDS             2665..3696
                     /gene="dctP"
                     /locus_tag="PK849_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE66297.1"
                     /translation="MKRFGKIAAIALVLVFVIGILSPAVAKEQWKFAIEEITGSVQDE
                     FAQFFKKKIMEKLDVDIEIYPVGVLGDSEDLTEQTMNGVLHFTMASPGHLGTFIPVVR
                     VFSLPFIWSDKMEVNKEVMKNSVAIYGLLEKEYEKHNLKLLGVFPEGWQVWTANKPLR
                     TPDDFKNFRMRIMGDPLLAEIYRAYGANAVQIPYPDLYSALQLKLADGNIQPYFAHEE
                     MKFYEQQDYFIDIREMPFVATFVVSLPWFQGLPDDQKKLIEEISKETIDFVFDMQEKM
                     NEERLQKMLKIKPSLQVIIPTAEEREVFKKLSLPVRDTYLKMAEPYGKDVLDTLVKEL
                     EEAEKKFAK"
BASE COUNT          720 a         1287 c         1129 g          743 t
ORIGIN      
        1 gaggacatga tgcggaacgt ggaggccgga atccgcggct tcctcgtcta cgacgaaggg
       61 gcgttgaagc tggtgaacca gatgcgcgcc gagggcttcc tcccgaagga gaccatcttc
      121 aagtggtccg ttttcggcgg tcagtgcagc cccgccgggg cgaagctcct ccgggagctg
      181 ggggccgaca cgatgaaccc gatctccgac gtctcgctcc cgatcctctc cagcatccgc
      241 agcaccgtcg atatgccgct cgacgtgtac atgatcatcg tcgactcctt cggcggcatg
      301 ttccgcgcct acgacgcgcc cgagatcgtc cgcgtggcct cccccgtcta cttcaagatc
      361 gagccgggaa cctccgaggg cgacatctac aagccgtggg tgaccgactc gtggcacgac
      421 gagttcatcc gcgccaaggt gaagatcgcc gcgacgctcc gcgagatcat ggaacgccgc
      481 gcccccgaac tgaagctctc cgaacagggg cccgccgacc tccgcctgcc cgtcgtcgac
      541 tgacatctac cgaacagccc gggagtatcc gcttcccggg ctgttttacc ttcggcctcc
      601 gggccgcgcc cgctcacctg aagggaggat cagccattga agaaactcga attgaaggga
      661 ctcgaaaaga tcgacgccgt gctgacatgg gtagaagaac ggattctggt gttcagcatt
      721 ctgttcatgg cggtcatact gatcgccaac gtgatctcgc gcaacttctt caactccagc
      781 atcaatgcgt cggaggagat cagccagttt ctcgtggtgc tggcgacctt cctggggaca
      841 agctacgccg ccaggaaggg gctccacata cggatgtcca tcctgaacga ctttctgaag
      901 gggaagccgc agaagatgct cgccctcttc gtctcgctgg caacggccgt catcatgttc
      961 tacatctcgt ggctcgcctt ccgctacgtg cgcagggtgg ggacgctcca tcgcgtcagc
     1021 cccatactcc aggttcctct ccactacatc tggtcggtgg ttcccgtcgg cttcctcctc
     1081 accggcgtgc agtacctcct gaccttcgcc agaaacctga cgagcggcga ggtctgggtc
     1141 tcctactacg tgaaggacga gaacgcggag cccgaggcgg ctccggcaga gggggggaac
     1201 tgagatgctc ctcatgatga tcggcgttat gtgcttcttc cttgtcctcg gcttcccgat
     1261 gatcatcccc ctgatcatcg cccccatagc ggtggccgcg gtctactacc cggcgctcga
     1321 cccgatgaac ctcgtccagc agatgctcgt cggcgttcgg ccgctctctc tggtcgccgt
     1381 cccgatgttc atcttcgccg cggacgtcat ctctaccggg caggtggcgc ggcggctcat
     1441 cgacttcgtc atggccttct tcggccacac gcgcggcggc ctcgccgtca ccaccacggc
     1501 ggcgtgcacc ttcttcggcg cggtctccgg ctccacgcag gccacggtcg tcgccatcgg
     1561 cggcaccatg cgcccctacc tgctccgcga cgggtacgac gactcgttct ccatcgcgct
     1621 catcatcaac gcggcgggaa tcgcgctcct gatccccccg agcatctcga tgatcatcta
     1681 cggcgtggtc acgggaagct ccgtcggcga actcttcatc gccggcatcg gcccggggct
     1741 gctcgtcttc cttctcttct cgctctactg cagcttcgcg gcgcggaaga tggacatcgt
     1801 ccgtacgccg aagatgccgc tccccgagcg ggtgaaggcg ctgcggaagg ccatcgtccc
     1861 catcggcttc ccgctgatca tcctcggcgg catctactcc ggaaagttca gtccgaccga
     1921 ggcggcggcg gtcgccgtcc tctacgccgt ggtcgtggag atcttcctct accggcacat
     1981 cggctggagg gatctctacc ggatcgccct ctccaccggc atcgtcaccg ccgtcgtctt
     2041 catcctcgtg gccgtcggcg cctccttctc gtggctcgtc ggctacgcgc gcatcccgga
     2101 ggccatcctc ccgccgatcc tgggggagag cccctccatg atcaagctgc tgctcgtcat
     2161 cgcggcctcc tacttcatcg gctgcatgtt cgtcgattcc atcgtggtca tcatgatcct
     2221 cgcgcccatc ttccacccga tcgcgacgaa gctgggcatc gacccgatcc tcatcggcgt
     2281 gctcgtcacc atgcaggcgg ccatcggcgc ggtgacgccg cccttcggct gcaacatctt
     2341 caccgcgatg gcggtcttca gacgaccgta tgcggacgtc gtcaggcgca tcatgccctt
     2401 cctcgcgctc tacatcctgg cgacgctcat cgtcatcttc ttcccctcgc tctccctctt
     2461 cctgagggac atggcgttcc ggtagcggcg ggaacgacgg gacgaaaggg ggcgttcgca
     2521 ggaaacatgc agaggaaggg cgcggcgaag cggatttctt gacgccgccg ggcgcgcatg
     2581 atttcgacgc ctcaagaggc gtcgggttca ggaaacgctg tacggaaaca acgtgttctc
     2641 aatctgaagg aggtgcctgc aggtatgaaa cggttcggaa aaatcgcggc gatagcgctg
     2701 gtgttggtct tcgtcatcgg aatactctct cccgccgtgg cgaaggagca gtggaagttc
     2761 gccatcgagg agatcacggg aagcgtccag gacgagttcg cgcagttctt taagaagaag
     2821 atcatggaga agctcgacgt cgatatcgaa atttaccccg tgggcgtcct cggagacagc
     2881 gaggacctga cggagcagac catgaacggc gtgctccact tcaccatggc ctccccagga
     2941 cacctgggaa ccttcatccc ggtggtccgc gtcttctccc tccccttcat ctggtccgac
     3001 aagatggagg tcaacaagga ggtcatgaag aacagcgtcg ccatctacgg ccttctcgag
     3061 aaggagtacg agaagcacaa cctgaagctc ctcggcgtct tcccggaggg gtggcaggtg
     3121 tggacggcca acaagccgct gcgcacgccc gacgacttca agaacttcag gatgcgcatc
     3181 atgggcgacc cgctcctcgc ggagatctac agagcctacg gcgcgaacgc ggtccagatc
     3241 ccgtaccccg atctctactc cgcgcttcag ctgaaactcg ccgacggcaa catccagccc
     3301 tacttcgccc acgaggagat gaagttctac gagcagcagg actacttcat cgacatccgc
     3361 gagatgccct tcgtcgccac gttcgtcgtc agcctgccct ggttccaggg gcttcccgac
     3421 gaccagaaga agctcatcga ggagatttcg aaggagacca tcgacttcgt cttcgacatg
     3481 caggagaaga tgaacgagga gcggctgcag aagatgctca agatcaagcc ctccctccag
     3541 gtgatcatcc ccaccgccga ggagcgcgaa gtcttcaaga agctctccct ccccgtgcgc
     3601 gacacctacc tcaagatggc cgagccctac ggcaaggacg tcctggacac cctcgtcaag
     3661 gagcttgagg aggccgagaa gaagttcgcg aagtagaaag caggcggggc gggggctcgc
     3721 tctcccgccc cttcgtgaat ggacgaattg gaggcgaacg ccgatgtact ttgaagttgc
     3781 ggagtttcac gaacgactga agaagacgaa agagagcatg ctgcggcaca agatcgaggt
     3841 cctcgtggtg agcgaccccg cgaacatgaa ctacctcac
//
LOCUS       DAOPKU010000269         3864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_26029_length_3864_cov_5.172749, whole genome shotgun sequence.
ACCESSION   DAOPKU010000269 DAOPKU010000000
VERSION     DAOPKU010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26029_length_3864_cov_5.172749"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK849_08985"
     CDS             complement(<1..444)
                     /locus_tag="PK849_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HPE66298.1"
                     /translation="MTAQKSFFVLGEVRDGRIHSVTRELTGKARELADSVGGRVTTVL
                     LSGGLADSPEDLFRYGADRVLSVEHPSMTLYNQEIETNVLAHVLREELPEVVLAGATT
                     SGRTTLPALASKLETGLTADCTGLEMDLETGLLLQTRPAIGGNVMA"
     gene            complement(457..1263)
                     /locus_tag="PK849_08990"
     CDS             complement(457..1263)
                     /locus_tag="PK849_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPE66299.1"
                     /translation="MKIAVLIKQVPDTDDVRMDPEKGTMIREGVGAIVNPLDLNALQA
                     ALDLKAASAEAAEASSSEVTVITMGPPKAHEALRECIATGADRGVLLSDRAFAGSDTW
                     ATAKVLAAAVEHTGPYDLVLAGEKATDGETGQVGPEVAVLLGIPFSTYVTGLSFTEGK
                     IEVHRMTEEGVQRQRLPFPCLLTVLHALNEPTMPTLAGKKRARRSEIPVLGISDIGLS
                     PEETGLKGSATRVVKIAYPTISRSTEMYDGKRADEGIDRLIEVLKDLAVI"
     gene            complement(1445..2014)
                     /locus_tag="PK849_08995"
     CDS             complement(1445..2014)
                     /locus_tag="PK849_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE66300.1"
                     /translation="MDSFLHKTDIAVQRILRIVVLFFFLALAFLLAYNVLLRLTNDLS
                     TFLTERGFSEAAGAVRGWLPITSMHWFDEIVELCFAALIFYGAASLWASGGHFSVGDW
                     ISPRLPYERLRNLYRVFVDLLGLAFLWVFFFYSLRLALRATELTTVFQIHKSALYCCM
                     PVSSFIMLLYSLRDVWNDGKRLFSPGRGA"
     gene            complement(2017..3306)
                     /locus_tag="PK849_09000"
     CDS             complement(2017..3306)
                     /locus_tag="PK849_09000"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE66301.1"
                     /translation="MLTFYIFATLIGFLLLNVPVAAAIGLTSVLFFAGLGQGAFLAML
                     PHRMYFGTTGFTLLAIPFFILAGNLMNTGGITKRIFRFASAMVGHIPGGLGQVNIVAS
                     VIFSGMSGSAVADAAGLGQIEHKAMVDDGYDPAVSASLVAVSSVIGPVIPPSIPFVLY
                     GAITGVSVARLFMAGFIPGILMSVGMMIALAILAKRRNYPCSACRAPRSDLWASFKGA
                     FWALMAPVIIIGGIMTGFFTPTEASVVVCAYAMILGFAYRDLHWKDMPDILFKSAGQA
                     VSLLFIIAAANFFGWLIIHQKIPDAIISNLMSYGATQAHVLLLIMGVILVLGCFLEGN
                     AIFLITLPIFIPVAKMFQIDLVNFGVVMTLLIMIGNLTPPVGMCLFAVDSFAKVGILP
                     LSKEVVPYLVVILGVTFVIAFVPDIALFLPRLMMGGM"
     gene            complement(3399..>3864)
                     /gene="dctP"
                     /locus_tag="PK849_09005"
     CDS             complement(3399..>3864)
                     /gene="dctP"
                     /locus_tag="PK849_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE66302.1"
                     /translation="YQAAIEALGGNVLTIAFGELVMAMRQGVVDGQENPIGVIYDLKL
                     YESQKFISIVNYLYSSMTHVVSKKVFDSLTEEQRNIIIEESNKARLLMRELVRKQEQE
                     QIADMRAKGIQIDTPDLTSFQAAMGPAYEKIKKNIGEENFNKWMEMAKATAE"
BASE COUNT          877 a         1151 c         1122 g          714 t
ORIGIN      
        1 cgccatgacg ttgccgccga tagccgggcg ggtctggagc agcaacccgg tttcgaggtc
       61 catctcaagg ccggtgcagt ccgcggtgag ccccgtctcc agtttcgagg ccaatgcggg
      121 gagcgtcgtc cgtccggacg tcgtcgcacc ggcgagcact acctcgggta gctcctcgcg
      181 aagcacgtgg gcgaggacgt ttgtctcaat ctcttggttg tacagcgtca tcgacgggtg
      241 ctccacggac aggaccctgt cggccccgta ccggaaaagg tcttcaggcg aatccgccag
      301 tcctcccgag aggagcaccg tcgtcacccg cccgccgacg ctgtcggcga gttccctggc
      361 cttccccgtc agttcgcgcg tcacggagtg aatgcgcccg tctctgacct ctccgaggac
      421 gaaaaagctc ttctgtgccg tcatagcgcg ttcctcctaa atcacggcga ggtctttgag
      481 gacctcgata aggcggtcga tcccctcgtc cgcacgtttc ccgtcgtaca tctccgtgga
      541 acgggagata gtaggataag cgatcttcac cacgcgggtc gcggagccct tgagccccgt
      601 ctcctcgggc gagagcccga tgtcggagat tccgagcaca ggaatctcgc tccgccgcgc
      661 ccgcttcttg ccggcgagcg tcggcatggt cggctcgttg agggcgtgaa gcactgtgag
      721 cagacacgga aatggcaggc gctgccgctg gacgccctcc tccgtcatgc ggtggacttc
      781 gatcttccct tcggtgaagg agagtcctgt gacgtacgtg ctgaagggta tgccaagcag
      841 caccgcaacc tccgggccaa cctgccccgt ctcgccgtcg gtcgccttct ccccggcgag
      901 aacgagatcg taggggccgg tatgctccac agccgcagcc aacactttgg ccgtagccca
      961 cgtatcgctt ccggcgaatg cgcggtcgct gagcagcacg ccccggtccg ctccggtcgc
     1021 gatgcactcg cggagcgcct cgtgcgcctt gggcggcccc atcgtaatca cggtgacctc
     1081 gcttgaagag gcttcggccg cctccgcaga ggccgccttc aggtcaagcg cagcctggag
     1141 agcgttcaaa tccagcgggt tcacgatggc cccgacgccc tcgcggatca tcgttccctt
     1201 ttcagggtcc atacgaacgt cgtccgtatc gggaacctgt tttattagca cggcaatctt
     1261 cacggaaaaa cctccttgca gtggaatgat acctgaaaca cctttcctcg ctgtacgata
     1321 ccacaaagtt caggagatcg gcgattccat cgagggaaaa acagctgatt ttcaggttcc
     1381 acaaaaaaga gggagcggaa acagcatccg ctcccaagca tacacaacgc aggatattcc
     1441 tgtttcaggc tccccttccc ggagagaaga ggcgctttcc gtcgttccag acgtcacgca
     1501 gcgaatagag gagcataatg aaggacgata cgggcataca gcagtacagc gcagacttat
     1561 gaatctggaa gaccgtcgtc aactccgtgg cgcgaagcgc cagacgcagc gagtagaaga
     1621 aaaagaccca cagaaacgcg agtccgagaa ggtcgacaaa cacacggtag agatttcgga
     1681 gtctctcgta cggaaggcgc ggcgaaatcc agtcccctac gctgaaatgg cctccgctcg
     1741 cccagaggga tgccgccccg tagaagatga gcgctgcgaa acagagctcc actatttcgt
     1801 cgaaccagtg catcgaagta attggaagcc accctctgac ggctcccgcc gcttcgctga
     1861 aaccgcgctc ggtcagaaaa gtcgacagat cgttcgtcag gcgcagcaaa acgttgtagg
     1921 ccagcaagaa agccagcgca aggaagaaaa aaagaacgac gattcggaga atacgctgga
     1981 cggcaatgtc ggttttatgg aggaaagagt ccattgttac attcctccca tcatcagacg
     2041 cggcaggaag agcgcgatgt cggggacaaa agcgatgacg aaggtcacgc cgagtatgac
     2101 gacgagatac gggacaacct cttttgaaag aggaagtatc ccgaccttcg cgaaactgtc
     2161 caccgcgaag aggcacatgc caacaggcgg cgtcaggttg ccgatcatga tcagcagcgt
     2221 catcaccacg ccgaagttga cgaggtcgat ctggaacatt ttcgcgacag ggatgaagat
     2281 cggcagcgtg atcaggaaga ttgcgtttcc ctcaaggaag cagccgagaa caagaatcac
     2341 acccataata agcagcagca cgtgcgcctg tgtggcgccg tagctcatca ggttcgagat
     2401 gattgcgtcg ggaatcttct gatgaatgat cagccacccg aaaaaattgg cggcggcgat
     2461 gataaagagc aggctcacgg cctgccccgc ggatttaaaa aggatgtccg gcatatcctt
     2521 ccagtggaga tcacggtagg cgaagccaag gatcatggcg tacgcgcaga ccactacgga
     2581 cgcctccgta ggcgtgaaga agccggtcat gatgccgccg atgatgatga caggcgccat
     2641 gagcgcccag aaagctcctt tgaagctcgc ccacaagtcg cttcgaggcg cccggcaggc
     2701 ggaacacgga taatttcgcc gtttcgcaag gattgcgagc gcaatcatca ttccaacgct
     2761 catcaggata ccggggataa accccgccat gaagaggcgg gcgaccgaga cgcccgtgat
     2821 cgccccgtag aggacgaagg gaatgctggg aggtatgacc ggtccgatga ccgatgagac
     2881 cgccaccaga gaggccgaga ccgcaggatc gtagccgtcg tccaccatcg ccttgtgctc
     2941 gatctgcccc agcccggccg cgtcggcgac cgccgagccg gacatcccgg agaagatgac
     3001 gcttgctacg atattcacct gccccaggcc tcccgggata tgcccgacca tggcgctcgc
     3061 aaagcggaag attctcttgg taattccgcc ggtgttcatc aggtttcccg cgaggatgaa
     3121 gaaaggaata gccaaaagcg taaacccggt ggtaccaaag tacattctgt gcggcagcat
     3181 ggcgagaaac gccccctgcc cgaggcccgc gaagaagagg accgaggtaa gacctatcgc
     3241 cgccgccacg ggtacattca agagtaaaaa gccgataagc gttgcaaata tgtaaaaagt
     3301 gagcatgaag tatcccccgt ttcactcggt gcggagaaga gaacggaagg gccaagcgcc
     3361 ctaaaacgga cgcccggccc ggcgattgcg aagactggct attcggctgt ggcctttgcc
     3421 atctccatcc acttgttgaa gttctcctca ccgatgttct tcttgatctt ttcgtacgcc
     3481 ggccccattg cggcctggaa ggaagtaaga tccggggtat ctatctgaat ccccttcgcg
     3541 cgcatatcgg cgatctgctc ctgctcctgt ttgcggacaa gctcgcgcat cagcagacgg
     3601 gccttgttcg actcctcgat gatgatgttg cgctgctctt ctgtcagcga atcgaagacc
     3661 tttttcgaaa cgacgtgcgt cattgagctg tagaggtagt tgacgataga gataaacttc
     3721 tgggattcgt agagtttaag atcgtagatg acgccgatgg ggttctcctg cccgtcgacg
     3781 accccctgcc gcatcgccat gaccaattcc ccgaaagcga tggtgaggac attaccgccg
     3841 agcgcctcga ttgccgcctg atac
//
LOCUS       DAOPKU010000270         3863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_26042_length_3863_cov_4.339548, whole genome shotgun sequence.
ACCESSION   DAOPKU010000270 DAOPKU010000000
VERSION     DAOPKU010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26042_length_3863_cov_4.339548"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PK849_09010"
     CDS             <1..682
                     /locus_tag="PK849_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPE66303.1"
                     /translation="GRVGRREEAAYAYFLYPEGRPLAAETLERLDAITSLNDEGAGYD
                     LALQDLRIRGSGDLLGTSQHGSGRGIADSHLYCTLLEEEIGKIRGVVPSAATVSAEIP
                     CLVPSFYIPQESIRIALYRRLLRVGSLDELHDLEKEVKDRFGTLPESLENLFSISFLR
                     SRGGFYGILSMECTRRETTLTGEGPLFDLLRTWKRWSGKEKKLSGPGGASGLRDVLLG
                     LRQIKQVL"
     gene            686..1486
                     /gene="mazG"
                     /locus_tag="PK849_09015"
     CDS             686..1486
                     /gene="mazG"
                     /locus_tag="PK849_09015"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPE66304.1"
                     /translation="METRSGHVFEELLAVMARLRGENGCPWDREQTMETLRTYIVEEA
                     YELVDAISRGETPDIVEEAGDLLLQVVFLSRIAEDEGRFTIDDVLRGLTEKLVRRHPH
                     VFGDEKADTSGQVLRKWEEIKGVEKEQRKKKDTSLLAGVPEGLPPLAKAARIQSKAAH
                     VGFDWEKGNLAPLYDKVDEEIEEIRNAVETGSADRIEDEMGDLLFAAVNLARHLEVNP
                     DSALGRANRKFSERFRIVESMVRETGRPWSSFSLDTLDTFWEQAKKSL"
     gene            1618..3054
                     /locus_tag="PK849_09020"
     CDS             1618..3054
                     /locus_tag="PK849_09020"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HPE66305.1"
                     /translation="METKSEPQPKKESRQIRKVGITDTTLRDAHQSLMATRMKVEDMA
                     EIIERMDDIGFHSMEVWGGATYDSCMRFLDEDPWERLRTMRRLFKKTKLQMLLRGQNV
                     VGYRHYADDTVREFVKRAVGNGLDIIRVFDALNDLRNMEVAADQIKKEGAHLQLAFSF
                     TLSPVHSLDAFGKLAKDMKSMGADSICIKDMAGIVSPVETAALVKTIKKETGLPVQIH
                     SHYTSGMASMAYYAGLEAGADVVDCAISPFALGTSQPPVESMVAALKGGDFDTDIELE
                     KLVPIAMHFKKVRDKYSKIFVDLGGVDINVLIYQIPGGMYSNLVSQLKDQNALARLPE
                     VLKEVPRVREEMGYPPLVTPTSQIVGTQATLNVLVGERWKVIPNEVKSYFRGEYGKPP
                     AEVDPEIRKKVIGDKEPITCRPAELIKPELEDARKAIAPWILQPEDVLSYVLFPSVAK
                     DFLMKKFAREMKVDVGVAELVDGAGYPV"
     gene            3155..>3863
                     /locus_tag="PK849_09025"
     CDS             3155..>3863
                     /locus_tag="PK849_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPE66306.1"
                     /translation="MARFAGNNDENLSEIEQRYPVSLLVRGNKVAVRGPDEVAVDVAY
                     GLLEQLHKIASKGHALHLADVRYAMDMIAEKGAVDLAPLFSEVICTTARGKPIRAKTE
                     GQRAYLETVRNNHIVFAVGPAGTGKTFLAVCHAVTLLKASAISRIVLVRPAVEAGESL
                     GYLPGDLREKVEPYVRPLYDAFYDLLSPERFLRYVDKGVIEIAPLAYMRGRTLNDSFI
                     ILDEAQNTTPEQMKMFLT"
BASE COUNT          874 a         1060 c         1211 g          718 t
ORIGIN      
        1 ggggcgcgtc ggccgcagag aagaggccgc atacgcctac tttctctacc cggagggacg
       61 gccgcttgcc gctgagacgc tggaacgcct cgacgcgatt acgtcgctca acgacgaggg
      121 cgccgggtac gatttggcgc tgcaggatct gcgcatccgc ggcagcggcg atctgctggg
      181 aacatcccag cacggaagcg ggcgcggaat tgccgattcg catctgtact gcactcttct
      241 tgaagaggag atcggcaaga tcaggggagt cgtcccctcc gccgcgacgg tgagtgcgga
      301 aataccctgc cttgtgcctt cgttttatat tccccaggag agcatcagga tcgcgctcta
      361 caggaggttg ttgcgcgtcg ggagcctgga tgagctgcac gacctcgaaa aagaggtcaa
      421 agatcgtttc ggaacgcttc ccgaaagtct tgaaaatctt ttttccatat cttttttacg
      481 ttcacggggc ggtttttatg gtatcttgtc tatggaatgt acacgaaggg aaacgacgct
      541 gaccggcgaa ggccccctct ttgatctgct ccggacgtgg aaacgctggt ccggcaagga
      601 gaaaaaactc tccggtcccg gaggggcaag cgggttgcgt gacgttctcc tcggacttcg
      661 acaaataaag caggtgctgt agaatatgga gactcgttcc ggacatgttt ttgaagagct
      721 tctggctgtc atggcgcgcc ttcggggaga gaacggctgc ccctgggaca gggagcagac
      781 gatggagacc ctcagaacgt acatcgtcga ggaagcgtac gagcttgtag acgcgatttc
      841 gcggggagag acgccggata tcgtcgagga ggcgggcgat cttttgctgc aggtcgtttt
      901 tctctcccgg attgcggaag atgaagggcg ttttaccatc gacgacgttc tgcgggggct
      961 gacggaaaaa ctggtccgac gtcaccccca tgtcttcggc gacgaaaaag ccgataccag
     1021 cgggcaggtg ctccggaagt gggaagagat caagggcgtg gagaaggagc aacggaaaaa
     1081 aaaggacacg tcgcttctcg cgggagttcc ggaaggattg ccgccgctgg cgaaggcggc
     1141 gcgtattcag tcgaaggccg cgcatgtcgg ttttgactgg gaaaaaggga atcttgctcc
     1201 attgtatgat aaagtagatg aggaaataga ggaaatccgg aacgccgtcg agacaggctc
     1261 ggcggacagg atagaggatg agatgggaga cctgctcttc gccgccgtga acttggcgag
     1321 gcatcttgag gtcaaccccg attcggcgct cggaagggcg aaccggaagt tctccgaacg
     1381 gttccggatc gtcgagtcca tggtgcgcga aacaggacgc ccatggagca gcttttctct
     1441 tgatacgctg gatacttttt gggaacaggc gaaaaaatcc ctgtaacagg gaaactgatc
     1501 gtgtacagga acacaaaaaa ttgaacgtat ggaggaaatg cgcacatgac caccccgaac
     1561 aagaaaaaga gcgagacggc ccccgcagca tcttccacgt cgcataagga gacgactatg
     1621 gagaccaaga gcgaaccgca gccgaagaaa gagtcgcggc agatcagaaa ggtcggcatt
     1681 accgacacga cgcttcgcga cgcgcaccag tcgctcatgg cgacgcgtat gaaggtcgag
     1741 gatatggccg agatcatcga gcggatggac gacatagggt tccattcgat ggaggtctgg
     1801 ggaggcgcca cctacgacag ctgcatgcgc ttcctcgacg aagacccctg ggagcgcctg
     1861 cgcaccatgc ggagactctt caagaagacg aagcttcaga tgctcctgcg cggccagaac
     1921 gtcgtgggat accgccacta cgccgacgac accgtgcgcg agttcgtcaa gagggccgtc
     1981 gggaacggac tcgacatcat ccgcgtcttc gacgcgctca acgacctgcg gaacatggag
     2041 gtcgctgccg accagatcaa aaaggaaggg gcgcatctgc agcttgcctt ctcgttcacc
     2101 ctttcgccgg tgcactccct ggacgccttc ggcaagctcg caaaggatat gaagtccatg
     2161 ggcgcggact ccatctgcat caaggacatg gcgggcatcg tcagcccggt ggagactgcc
     2221 gcgcttgtca agacgatcaa gaaagagacg ggactccccg tgcagataca ctcccactac
     2281 accagcggca tggcctccat ggcctattac gcagggctcg aggcgggagc ggacgtcgtc
     2341 gactgcgcca tctcgccgtt tgccttgggc acaagccagc cgcctgtgga gtcgatggtg
     2401 gccgcgctca agggcggcga tttcgacacg gatatcgagc tcgaaaagct cgtgccgatc
     2461 gcgatgcact tcaagaaagt gcgcgacaag tactccaaga tcttcgtgga tttgggcggc
     2521 gtggacatca acgtgctcat ctaccagatc cccggaggca tgtactccaa cctcgtgagc
     2581 cagctcaagg atcagaacgc ccttgcccgg cttcccgagg tgctcaagga agtgccccgc
     2641 gtccgcgagg agatggggta ccccccgctc gttacgccca ccagccagat cgtaggcacg
     2701 caggcgacgc tcaacgtgct cgtcggcgag cgctggaagg tcattccgaa tgaagtgaag
     2761 agctacttcc gaggcgagta cggcaagcct cccgcagagg tcgacccgga aatccgtaaa
     2821 aaggtcatcg gcgacaaaga gcccatcacc tgccgtcctg cggagctgat caaacctgag
     2881 cttgaagatg cgcggaaggc tatcgctccg tggattctcc agcctgagga cgtgctcagc
     2941 tacgttctct tcccgtcggt ggccaaggac ttcctgatga agaaattcgc gcgggagatg
     3001 aaggttgacg tgggcgtcgc ggaacttgtc gacggcgccg gatatcctgt ctaaagggag
     3061 cgtgcatcgg agaagaatgt acattgatgt agaaaacaaa gcggaggaga ccggagcatt
     3121 tctgcgcgag cttgatttca gcgacataga gactatggcg cgtttcgcgg ggaataatga
     3181 cgaaaatctc tccgagatag agcagcgcta tcccgtgagc ctccttgtac gggggaataa
     3241 agtcgcggta cgcggtccgg acgaggtcgc cgtggacgtg gcgtacgggt tgcttgagca
     3301 gctccataaa atagcctcga aggggcatgc gctccacctg gcggatgtcc gctacgcgat
     3361 ggatatgata gcggagaagg gggcggtcga tctggccccc ctcttctccg aagttatttg
     3421 caccacggcc agaggaaaac cgatccgggc gaagaccgag gggcagcgcg cgtaccttga
     3481 gaccgtccgc aataatcata ttgtctttgc cgtcggacca gccggaaccg ggaagacctt
     3541 tcttgccgtc tgccacgccg tgacgctgct caaggcctcg gcgatcagca ggatcgtgct
     3601 tgtgcgtccc gccgtggagg cgggggagag cctcggctat ttgcccggcg accttcggga
     3661 gaaagtggag ccgtacgtgc gcccgcttta tgacgcattc tacgacttgc tctctccgga
     3721 gcgctttctc cggtatgtgg acaagggggt catcgaaata gccccgctgg cgtacatgcg
     3781 gggaagaacg ctcaacgaca gcttcatcat cctcgacgaa gcgcagaaca ccacgcccga
     3841 gcagatgaag atgtttctca ccc
//
LOCUS       DAOPKU010000271         3856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_26098_length_3856_cov_5.849513, whole genome shotgun sequence.
ACCESSION   DAOPKU010000271 DAOPKU010000000
VERSION     DAOPKU010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26098_length_3856_cov_5.849513"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="PK849_09030"
     CDS             <1..1442
                     /locus_tag="PK849_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66307.1"
                     /translation="QRRTFELALAKEEAEKANKAKSSFLANMSHEIRTPLNAILGFGA
                     VLERDPSLSPKQAEYVNAVSRSGRHLLKLINDILDMSRIEADRLVLNPTDFNLRDLLA
                     DMRMMFGARAEEKHLQLIMEIQETLPVYARGDEAKVRQIFVNLLGNAVKFTLSGGVAV
                     RARMDKPREAESASFLLVEVEDTGPGIPPEDMKKIFDPFRQSDAGKQAGGSGLGLSIS
                     KRLAELMGGTLTAVSTVGKGTCFRLRLPLHTAEHTAEHPRTALRSIVGAESASSYRIL
                     LVDDIKENRDVLRAVLEPLGFSLMEAVDGAEGVKLFEEWAPHAVLMDMRMPVMDGYEA
                     TRRIKETPEGAAVPVIAITASAFEDEVQNVLNSGADYCIRKPLRPEELFEILGEALGL
                     RYVYGADTAEHEEKPHEITAGDVARLPESLTSAMRTAVEEGDILQLEALINTIQEVDA
                     PLADGLRRLARRYDYETLTDLLPEEVESP"
     gene            1439..2548
                     /locus_tag="PK849_09035"
     CDS             1439..2548
                     /locus_tag="PK849_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HPE66308.1"
                     /translation="MNDHNEQTTIMLVDDTPANLELLRAMLHKKGYKVLVFPGGEPAL
                     RALERKEPDLILLDIMMPEMNGFEVCGRLKEDERLREIPVIFISALDDTQSKVRAFTA
                     GGVDYVTKPFQPEEILARVQTHVTLRQVRRELEKHMSELEELVREKVKEISDSQMATI
                     HAMSKLAEYRDDQTGRHIERTRELCAMLAGKLRENPRYALLIDDAFVENIYHASPLHD
                     IGKVGIPDAILLKPGKLTEEEFEIIKRHPRIGATTLSWVRSKYPQNAFINMGIALALF
                     HHERWDGGGYPDGLAGEDIPLSARIMALVDVYDALRSDRPYKEAFPHETACRIIREGN
                     GTHFDPSVVDAFFAVEQEFEQICDHMTNEGAEGIL"
     gene            2585..>3856
                     /locus_tag="PK849_09040"
     CDS             2585..>3856
                     /locus_tag="PK849_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE66309.1"
                     /translation="MKTTTRTPGSLPTGQTAEKGFRSSWNGSRYREFLLLIAPLLLFS
                     AYPLLRSFEHAGNSGVFGGVRMTGALIALSAGCAFIARFAGPGRRFYLFAGLAFFVAG
                     AEDLAAGFLELAGASLFQAPREAVLRSSIAFRTLTPLLLMAPLVLSAGEGVPRRPGRE
                     LLPAPLSVPLIIALTLYAALNGSPLPFHTLLSRPLDFLSAALFAAALIVYVREFRSSG
                     ESIAWWLGLSIGVMLAGQSLLLFSRGIYDPFFAAARVCSILGYAASLLGFSLHQLSVE
                     DDQNWTVFRMSAEVDRINAAYLEDVVLEKIREVADSHMATILAMSKLAESRDDETGRH
                     IERTQELCRVLARKMRELPRYASVIDDAFIENIYHAASLHDIGKVGIPDAVLLKEGAL
                     SEEEREMIRRHPGIGAATLERVRSQHPGNEFL"
BASE COUNT          774 a         1228 c         1208 g          646 t
ORIGIN      
        1 tacagcgacg caccttcgaa cttgccctgg ccaaagagga ggcggagaag gccaacaagg
       61 ccaagagctc cttcctcgcc aatatgagcc acgagatccg cacgccgctc aacgccatcc
      121 tcggcttcgg cgcggtgctc gaacgggatc cgtcgctctc tccaaaacag gccgagtacg
      181 tgaacgccgt cagccgaagc ggacggcatc ttctgaagct gatcaacgat atcctcgaca
      241 tgtcgaggat cgaagccgac cggctcgtgc tcaacccgac cgacttcaac cttcgcgatc
      301 tcctggccga catgcggatg atgttcggcg cccgcgccga ggaaaaacat ttgcagctca
      361 tcatggagat tcaggagact cttccagtgt acgcgagggg cgacgaggcc aaggtacggc
      421 agattttcgt caacctcctc ggaaacgccg tgaaattcac gctctccggc ggcgtcgcgg
      481 tacgcgccag aatggacaag ccgcgcgaag cggaaagcgc ttccttcctc ctcgtcgagg
      541 tcgaggacac cggaccgggc attccccctg aggacatgaa gaagatcttc gaccccttcc
      601 gacaatcgga cgccggaaaa caggcgggag gttcgggact gggactgtcc atcagcaaac
      661 ggcttgcgga gctgatgggc ggcacgctca ccgccgtaag caccgttgga aaaggaacgt
      721 gcttccgcct gcgccttcct ttgcacacgg cggaacacac ggcggaacat ccgcggacag
      781 cccttcgcag catcgtcggc gctgaatccg cctcttccta ccggatattg ctggtcgacg
      841 acatcaaaga aaacagggac gtactgcgcg ccgttctcga acccctgggc ttcagcctga
      901 tggaagcggt cgacggggcc gagggggtga aactcttcga agaatgggcg ccgcacgccg
      961 tgctgatgga tatgcgcatg cccgttatgg acggctacga ggcgacgcgc cgcatcaagg
     1021 agacccctga gggcgccgca gtcccggtca tcgcaatcac agcgagcgcg tttgaagacg
     1081 aggtgcagaa tgtcctgaac agcggagccg actactgcat acgcaaacct ctccgaccgg
     1141 aagagctgtt cgagatactc ggcgaggccc tcggactgcg ctacgtctac ggagccgaca
     1201 ccgccgaaca cgaagagaag ccccatgaaa tcacggccgg ggacgtggcg cggctgcccg
     1261 aatcgctgac gtccgccatg agaaccgccg tcgaggaggg ggacatcctc cagctggaag
     1321 cgttgatcaa cacgattcag gaagtcgacg cgccgcttgc ggacggactg cggcgtcttg
     1381 cccggagata cgactacgaa acgctcacag atcttttacc ggaggaggtg gaatccccgt
     1441 gaacgatcac aacgaacaga cgaccatcat gctggtcgac gataccccgg ccaacctcga
     1501 acttcttcgg gcgatgctgc acaaaaaagg gtacaaggtg ctggtcttcc ccggcggaga
     1561 accggcgctc cgcgcgctcg aaaggaaaga acccgatctg attctgctcg acatcatgat
     1621 gccggagatg aacggttttg aagtatgcgg ccggctgaaa gaggatgaac gcctccggga
     1681 aattcccgtg atcttcatca gcgcgctgga cgacacgcaa agcaaggtga gggcctttac
     1741 cgccggaggc gtggactacg tgaccaaacc gtttcagccg gaggagatcc tggcgagagt
     1801 gcagacgcac gttacgctgc ggcaggtgcg ccgcgagctt gaaaaacaca tgtcggaact
     1861 cgaagagctt gtcagggaga aggtgaagga gatctccgac tcgcagatgg cgacgatcca
     1921 tgcgatgtcg aagctggcag agtaccggga cgaccagacc gggcggcaca tcgaacggac
     1981 gcgggaactc tgcgccatgc tcgccggaaa gctccgcgag aaccctcgct acgccctcct
     2041 catcgacgac gccttcgtcg agaacatcta ccacgcctcg cccctgcacg acatcggcaa
     2101 ggtgggcatc cccgacgcga tcctgctgaa accgggaaag ctcaccgagg aggagttcga
     2161 gatcatcaag cggcacccgc gcatcggggc gacgacgctg agctgggtgc gcagcaagta
     2221 cccgcagaac gcgttcatca acatgggcat cgccctcgca ctcttccacc acgagcggtg
     2281 ggacgggggg ggctacccgg acggcctcgc gggcgaggac attcctctga gcgcgcgcat
     2341 catggcgctc gtggacgtct acgacgcgct ccgctccgac cggccgtaca aggaggcgtt
     2401 cccgcacgaa acggcctgcc ggatcatcag ggaggggaac gggacgcact tcgacccttc
     2461 ggtcgtcgac gccttcttcg ccgtcgagca ggaattcgag cagatctgcg accacatgac
     2521 gaacgagggc gcggagggca tactgtaacg ccggccggcc gtgccggggc ggaagggtga
     2581 catcatgaaa acaacaacgc gaacacccgg gagtctcccg acgggacaga ccgcggaaaa
     2641 gggattccgc tcctcgtgga acggaagccg gtatcgcgaa ttcctgctgc tcattgcgcc
     2701 tctcctgctg ttttcggcgt atccgctgct tcgctcgttc gaacatgcag gaaattccgg
     2761 cgtgttcggg ggcgtcagga tgacgggcgc gctgatcgcc ctttcggcgg gctgcgcttt
     2821 catcgcgaga ttcgccggcc ccggcagacg cttttacctg ttcgcggggc tcgctttctt
     2881 cgtcgccgga gcggaggacc tcgcggccgg cttccttgag ctggcggggg catccctctt
     2941 tcaggcgcct cgcgaagccg tcctccggag ttcgatcgcc ttccggacgc tgacgccgct
     3001 gctgttgatg gcgccgctcg tcctttccgc cggggagggc gtgccccggc gccccggacg
     3061 ggaactcctg cccgcgccgc tctccgtgcc gctgatcatc gcgctcacgc tgtacgcggc
     3121 gctcaatggc tcgccgctcc cgttccacac acttctctcc cgtcctctgg actttctctc
     3181 cgccgccctg ttcgcagcgg cgctgatagt gtatgtccgc gagttccgaa gttcgggcga
     3241 gagcatcgcg tggtggctcg ggctctccat cggcgtcatg ctggccggtc agtcgctgct
     3301 gctcttctcg cgggggatct acgacccgtt cttcgccgcc gcaagagtct gctcgatcct
     3361 cgggtatgcc gcctcgctgc tgggtttctc gctccaccag ctctcggtgg aggacgacca
     3421 gaactggacg gtcttccgga tgagcgccga ggtggaccgg atcaacgccg cctatctgga
     3481 ggacgtggtg ttggagaaga tacgcgaagt cgccgactcg cacatggcga cgatcctcgc
     3541 catgtcgaag ctggcggagt cgagggacga cgaaacggga agacacatcg agcgcacgca
     3601 ggagctgtgc cgcgttctgg cccgcaagat gcgggaactc ccccgctacg cgtccgtgat
     3661 cgacgacgcg ttcatcgaga atatctacca tgcggcgtcg ctgcacgaca tcggcaaggt
     3721 aggcatcccc gacgccgtcc tcctgaaaga gggcgcactc tccgaagaag agagggaaat
     3781 gatacgacgg caccccggaa tcggggcggc gacgctggag cgtgtgcgca gccagcaccc
     3841 cgggaacgag tttctc
//
LOCUS       DAOPKU010000272         3839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_26269_length_3839_cov_4.115222, whole genome shotgun sequence.
ACCESSION   DAOPKU010000272 DAOPKU010000000
VERSION     DAOPKU010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26269_length_3839_cov_4.115222"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PK849_09045"
     CDS             <1..255
                     /locus_tag="PK849_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPE66310.1"
                     /translation="VFVGPYDLSQSFGIPGQIYHPVMVEAVAKALAVAKRAGKPAGIF
                     VSSVEEAKARIEQGFAYVAYNTDSLLFAELCRNIVKGVKG"
     gene            complement(535..1665)
                     /locus_tag="PK849_09050"
     CDS             complement(535..1665)
                     /locus_tag="PK849_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPE66311.1"
                     /translation="MSGRKYTIARVPGDGIGPEVIGETSKILEAAGSKHGFSISWADY
                     PFGADHYLKTNEILPASAVDEMAPMDALLLGAVGDPRVKPGILERGILLHLRFHFDQY
                     VNLRPAKSYPRVPLPIQPKKGPTLNTLVVRENTEDFYVGIGGKTEKGKTVVDLATTRG
                     LYSMSGTLEGAFSDGVEGAFQLGVASEPGVRRVVAYAARAAKARGEEKITLASKSNAL
                     PQIYGFWEDVAKDEAAKQGVGLSIVNVDAMCYHLVRTPDLYGVVVAPNMFGDIVSDLL
                     AGLAGGLGVAAGADIGDNLSMFEPIHGSAPDIAGTGKANPIAAILTGALMLEHIGERD
                     AAQGVENALTEFLAHAETRDLPVEFGGEATTSAVGEAIAKRV"
     gene            complement(1662..2174)
                     /locus_tag="PK849_09055"
     CDS             complement(1662..2174)
                     /locus_tag="PK849_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPE66312.1"
                     /translation="MSKIFKGNAWKYGDNIDTDVIIPARHLVTSDEAELGKHCMEDID
                     KDFVKKVAPGDMIVGGENFGCGSSREHAPIAIKGAGCSCVIAASYARIFFRNAINVGL
                     PIFECPEAAASIETGDEIEVDPGAGTIVNKTKGKTFTVAPFSPFLQDLIQQGGLIPYV
                     SKELARRKGA"
     gene            complement(2177..3466)
                     /locus_tag="PK849_09060"
     CDS             complement(2177..3466)
                     /locus_tag="PK849_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPE66313.1"
                     /translation="MGRTLASAIIAAHSIEGAREGEICKVNVDFAFANDITAPPAIVS
                     FKEMGAEKVFDKERCAVLPDHFTPNKDIASAEQTKKGREFALSQGMLYWEVGRVGIEH
                     AFLPEKGYILPGDIVIGADSHTCTGGALGALSTGVGSTDLAAVWALGETWLKVPPTIR
                     VNYEGKRPAWVSGKDMILSLIGRIGVQGARYMALEFGGDGVEALPMDDRFTIANMAIE
                     AGGKAGLFVPDAKMLAYANARAARKFSPVYPDADAAYFRTETFDMEKMVPVAAMPHSP
                     DNVKPVRECGSPEIHQVFIGACTNGRIRDIAAAAKMLEGKQVAAGVRCIVIPASYEVY
                     NQALDMGYLRTFTEAGAVVCTPTCGPCLGGHMGILAAGERCVSTSNRNFVGRMGSTKS
                     ELILASPLTAAASAITGRLTDPRDVASPDFFKKLEEE"
     gene            complement(3545..>3839)
                     /locus_tag="PK849_09065"
     CDS             complement(3545..>3839)
                     /locus_tag="PK849_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPE66314.1"
                     /translation="GNGPVDAAYRAIRRIVGIEPELENYAIQAVNESSDAIGEARVTL
                     DLGGVRVNGRGASTDVIEASIKAYIDGINRLFAAQARTAGQEAQAAEEARKAV"
BASE COUNT          600 a         1248 c         1238 g          753 t
ORIGIN      
        1 gtcttcgtgg ggccgtacga cctttcccag tcattcggca ttccggggca gatctaccac
       61 cccgtgatgg tcgaagccgt agcgaaggcg ctcgcagtcg ccaaacgcgc cgggaagccc
      121 gccggtatct ttgtctcctc cgtcgaggag gcgaaggcga gaatcgagca ggggttcgcc
      181 tacgtcgcgt acaataccga ctcgctgctc ttcgccgagc tctgccggaa catcgtgaag
      241 ggcgtcaagg ggtaggagca gataacgcgc tcctgaatcg ggacaggcgg tacggcggaa
      301 gagcgttccc gctctgaaga aacgcgtatg cgcagaaaaa acgtacaagg ataccggacg
      361 ccaaggagcg ctgcagcctc tcctgtctgt acgaaatcgt cgcgccctct ttatcggatg
      421 cggagcctgc gttggtccgc tcctcttcgc tcttccgccg cacactgatt ctacgaggag
      481 agcatcgaca aaaagcgccg ctccgtgtcg acgggagcgg cgcttttcga tagtttacac
      541 ccgtttcgcg atggcttctc ccacggcgga ggtggtcgcc tcgccgccga actcgacggg
      601 gaggtcgcgc gtttcggcgt gcgccaggaa ctccgtcagc gcgttctcga cgccctgcgc
      661 ggcgtcgcgc tcgccgatgt gctccagcat gagcgccccg gtgaggatgg ccgctatggg
      721 gttcgccttc cccgttcccg cgatgtcggg ggcggagccg tggatcggtt cgaacatcga
      781 aagattgtcc cctatgtccg ctccggcggc gacgccgagc cctccggcga gccccgcgag
      841 aaggtcgctg acgatgtcgc cgaacatgtt cggcgcgacc acgacgccgt agaggtcggg
      901 cgtgcgcacc aggtggtagc acatggcgtc cacgttcacg atggagagcc cgacgccctg
      961 tttcgccgcc tcgtccttcg cgacgtcctc ccagaagccg tagatctgcg gcagggcgtt
     1021 cgatttcgag gccagcgtga tcttctcttc gcctcgggcc ttcgcggcgc gcgcggcgta
     1081 cgcgaccacg cggcggacgc ccggttccga ggccactccg agctggaacg cgccctccac
     1141 gccgtccgag aacgcgcctt cgagcgttcc gctcatggag tagagccctc gcgtcgtcgc
     1201 aagatcgacc acggtcttcc ccttttccgt tttgccgccg atgccgacgt agaagtcttc
     1261 cgtgttctca cgtacgacga gggtgttcag ggtcggcccc ttcttcggtt ggatgggcag
     1321 gggcacgcgg gggtagctct tcgccgggcg gaggttcacg tactggtcga agtggaagcg
     1381 aaggtgcagc agaataccac gctcgagaat tcccggtttt acgcgcggat cgccgaccgc
     1441 tcccagcagc agcgcgtcca tcggcgccat ctcgtccacc gccgacgcgg ggaggatctc
     1501 gttcgtcttc aggtagtggt ccgcgccgaa ggggtagtcg gcccacgaga tggagaaacc
     1561 gtgtttcgag cctgctgctt caaggatctt ggacgtctcg ccgatgacct cggggccgat
     1621 gccgtccccg gggacgcggg ctatggtgta cttcctgccg ctcatgcgcc cttcctccgt
     1681 gcaagctcct tgctgacgta ggggatgagg ccgccctgct ggatgagatc ctggaggaag
     1741 ggggagaagg gggcgacggt gaaggtcttc cccttggtct tgttcacgat ggtccccgcg
     1801 ccggggtcga cttcgatttc gtccccggtc tcgatggagg ccgcggcctc cggacactcg
     1861 aagatcggca ggccgacgtt gatggcgttg cggaagaaga tgcgcgcgta cgaggccgcg
     1921 atgacgcagc tgcaccccgc gcccttgatg gcgatgggcg cgtgttcgcg cgaagagccg
     1981 cagccgaagt tctcgccgcc cacgatcatg tcgcccggcg cgaccttctt cacgaagtcc
     2041 ttgtcgatgt cctccatgca gtgcttgccc agttccgcct cgtcgctggt gaccagatgc
     2101 cgggcaggga tgatgacgtc ggtgtcgata ttgtcgccgt atttccacgc gtttcctttg
     2161 aatatcttgg acatggctac tcttcctcca gtttcttgaa gaagtcgggg gaggctacgt
     2221 cgcggggatc cgtgagcctg cccgtgatgg cgctggccgc agccgtcagc gggctcgcca
     2281 ggatgagttc gctcttcgtg cttcccatgc gccccacaaa gttccggttg cttgtggaga
     2341 cgcagcgctc gcccgcggcg aggatgccca tgtggccgcc caaacagggg ccgcaggtcg
     2401 gggtgcagac gaccgcgccc gcctccgtga aggtacgcag ataccccata tcgagcgcct
     2461 ggttgtagac ctcgtacgag gcggggatga cgatgcagcg cacgccggcc gccacctgtt
     2521 ttccttcgag catcttcgcg gcggccgcga tgtcgcggat gcggccgttg gtgcatgcgc
     2581 cgatgaagac ctggtggatc tccggagagc cgcattcgcg gaccggcttg acattatcgg
     2641 gagagtgcgg catggcagct actggaacca tcttctccat gtcgaacgtc tcggtacgga
     2701 agtacgccgc atccgcgtcg gggtagaccg gagagaactt tcgggccgcc cgcgcgttcg
     2761 cgtacgcaag catcttcgcg tcgggcacga agagtccggc tttgccgccc gcctcaatcg
     2821 ccatgttggc gatagtgaag cggtcgtcca ttggaagcgc ttccactccg tcgccgccga
     2881 actcgagcgc catgtagcgt gctccctgta cgccgatacg gccgatgagc gagaggatca
     2941 tgtccttgcc cgacacccat gcggggcgct tcccctcata gttcacccgg atggtggggg
     3001 gaaccttcag ccacgtctca cccagcgccc agacggcggc gaggtcggtg ctgccaacgc
     3061 ccgtggagag agcgcccagc gcgccgcctg tgcaggtgtg gctgtccgcg ccgatgacga
     3121 tgtcgccggg gagaatgtac cccttctcgg ggaggaacgc gtgttcgatg cccacgcggc
     3181 cgacctccca gtagagcatc ccctgcgaga gggcgaactc gcgccctttc ttggtctgtt
     3241 ccgcagaggc gatgtccttg ttcggcgtga agtggtcggg gagtaccgcg caccgctcct
     3301 tgtcgaaaac cttctccgct cccatctcct tgaaggagac gatggccgga ggagccgtga
     3361 tgtcgttcgc gaaggcgaag tcgacgttca ccttgcagat ttcgccctcc cttgccccct
     3421 cgatcgagtg ggcggcgatg atggcgcttg ccaatgttct tcccatgtgt acacacgcac
     3481 tcctttccaa tgatgcccgg tttcgatgcc cccgccgccg cgcttctgcg tgagcggggg
     3541 aaaactatac cgccttccgc gcctcttcgg ccgcctgcgc ctcctgcccc gcggtgcgcg
     3601 cttgcgccgc gaaaagacgg ttgataccgt cgatgtacgc cttgatgctc gcttcgatga
     3661 cgtccgtgct ggcgcccctg ccgtttaccc tgacgccgcc gaggtcgaga gtcactctcg
     3721 cctcgccgat ggcgtcggag gattcgttga cggcctggat tgcgtagttc tccagctccg
     3781 gctcgatgcc gacgatacgt ctgatcgccc tgtaagccgc gtccaccggg ccgttgccc
//
LOCUS       DAOPKU010000273         3837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_26285_length_3837_cov_4.417768, whole genome shotgun sequence.
ACCESSION   DAOPKU010000273 DAOPKU010000000
VERSION     DAOPKU010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26285_length_3837_cov_4.417768"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PK849_09070"
     CDS             <1..639
                     /locus_tag="PK849_09070"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE66315.1"
                     /translation="ILALMTMVIIMGGILSGVFTATEASVVAAFYSFIVGRFVYGELK
                     WSAIPEILVKTGITTGVVVLCIATASSFGWILAAEQIPDKIAKAVFALSENRLVILFL
                     MNALMLFMGTFLDISPIIIILVPILFPIARQLGVTPIHFGIMTIVNMAIGQCTPPVGI
                     SLFVATGIAKAKLGEILGAYMKYLLTMIVVLAIITIFPQIITFLPQMVMGLK"
     gene            801..1781
                     /locus_tag="PK849_09075"
     CDS             801..1781
                     /locus_tag="PK849_09075"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPE66316.1"
                     /translation="MSKKLFALALLVMFTVILTASAASAAVVLKLSNVLPDGNPTTES
                     LVFFAEKVKEKTGGEVEVRLFNNSALGGQREALESMQAGTLEMCMANAGPMAQFVPTM
                     DVLSLPYVFQSTEHMFKALNGKAGEMIKADIDKAGFVFLYWGDGGSRNLINNKKPITK
                     PEDLKGLKIRVMDSRLMVNTLNAMGAIATPMAQGEVYSALQQGVLDGWENNPVTLLTL
                     KLYEVSDYFSWTQHFMVPDLVLISKQAFEKLTPEHQKALLEAGRESEAKQREYWNAFV
                     GKTVEELKSHNVKFNDVDIAPFVQSVQPVWEDYTKKFGTELLDLITSAAK"
     gene            1857..2525
                     /locus_tag="PK849_09080"
     CDS             1857..2525
                     /locus_tag="PK849_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HPE66317.1"
                     /translation="MARWTTDKELFSLMKNELFTALVGDVLDTMGLTKQFLPPRIRPL
                     LPETALAGRAMTVLEADCCGAGRKPFGLMFEALDDLKEDEVYICAGGSPDFAHWGGLM
                     TIRAKHLNAAGAVTHGYTRDTRQVLELDFPVFSWGTYAQDQGARGRVIDWRCPVEFVN
                     GVRVETGDLLFGDMGGVVVVPRAVESEVIEKALEKAEGENLVAKAIMGGMSTVEAFAT
                     FGIM"
     gene            2557..3570
                     /locus_tag="PK849_09085"
     CDS             2557..3570
                     /locus_tag="PK849_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HPE66318.1"
                     /translation="MRSAKLVAPRQIEIFEDRMPLPGEGEVLVRVKAAGICGSDLHIY
                     QGERADVTLPRILGHELVGEVESSGGGADRFSKGDRVTIDPVVSCGSCVSCKRGYDNL
                     CSTVKCLGVQVDGGYCDFIVVPEEKVYALPSSLPWEKAALIEPFSIAAEVLSRSEVRE
                     GDKVLVLGAGTIGLCILQAMKLAGAEVLTSDFVESRLEKAKELGADRVVNSKTGDLSA
                     ALREFTGEFGADVVVEAVGIPRLLEESLGYAAPGGRIVVLGFNPEPAKIPEVLVVKKE
                     LKIIGSRMNCRKFPQVIEWFEKGLVNPEPLISAKYPFEKINDAFKDILRDPQKYLKVL
                     ITY"
     gene            3616..>3837
                     /locus_tag="PK849_09090"
     CDS             3616..>3837
                     /locus_tag="PK849_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPE66319.1"
                     /translation="MILDLFKLDGKTAIVTGARKGLGYGIAEGLAEAGANIVGVGPIA
                     MPEIEARVKALGRKFLHVEADLTSQARIPD"
BASE COUNT          748 a         1017 c         1312 g          760 t
ORIGIN      
        1 attcttgccc tgatgacgat ggtcatcatc atgggaggca ttctctcggg ggtcttcacc
       61 gcgacggagg cgtccgtggt ggcggcgttc tactcgttta tcgtagggcg gttcgtttac
      121 ggcgagctga agtggagcgc gattccggag attctggtga agacgggaat caccaccggc
      181 gtggtggtgc tgtgcatcgc cacggcgtcg agcttcgggt ggatacttgc cgcggagcag
      241 atccccgaca agatcgcgaa ggcggtcttc gccctctcgg agaaccggct ggtcatcctc
      301 tttctgatga acgccctgat gctcttcatg gggaccttcc tcgacatctc cccgatcatc
      361 atcatcctcg tccccattct cttcccgata gcgcgtcagc tcggcgtgac gccgattcac
      421 ttcggcatca tgaccatcgt gaacatggcg atcggtcagt gtacgcctcc tgtgggcatc
      481 tctctcttcg tggcgacggg catcgcgaag gcgaagctgg gggagatcct gggggcgtac
      541 atgaagtacc tgcttaccat gatcgtcgtg cttgcgatca tcaccatctt cccgcagatc
      601 attacgttcc tcccgcagat ggtgatggga ttgaagtaat tctgcgacgg cggggcttct
      661 cgcccggccg gacatggaac gaagggcggt gggaacaggc ggcggagttt gctaagaaca
      721 gcgcgaacag gattcacccc tcggcggcga cgagggaaag aacagcagtc catcagctga
      781 tacccgaagg aggtagcatt atgtcgaaaa agttgtttgc tcttgcgctc cttgtgatgt
      841 tcacggttat tctgactgca tccgcggcgt ccgccgcagt tgttctgaag ctgtccaacg
      901 ttcttcccga cggcaatccg acgacggagt ccctggtttt cttcgccgag aaggtgaagg
      961 agaagacggg cggcgaggtg gaagtgcgcc tcttcaacaa cagcgcgctc ggcggtcagc
     1021 gcgaggcgct tgaaagcatg caggcgggca cgctcgagat gtgcatggcc aacgccgggc
     1081 cgatggccca gttcgttccc acgatggacg tcctctccct cccctacgtc ttccagagca
     1141 cggagcatat gttcaaggcg ctcaacggga aggccggaga gatgatcaag gcggacatcg
     1201 acaaggccgg attcgtcttc ctctactggg gcgacggcgg ttcgcgcaac ctgatcaaca
     1261 acaagaagcc aatcacgaag cccgaagact tgaaggggct gaagatccgc gtcatggaca
     1321 gccgcctgat ggtgaacacg ctcaacgcga tgggcgccat cgccacgccg atggcgcagg
     1381 gcgaagtgta cagcgcgctg cagcagggcg tgctcgacgg atgggagaac aaccccgtca
     1441 cgctgcttac gctgaagctc tatgaggtgt ccgactactt ctcgtggacg cagcacttca
     1501 tggtgcccga ccttgtgctc atcagcaaac aggcgttcga aaaactgact cccgaacatc
     1561 aaaaagcgct gttggaggcc gggcgcgagt ccgaggccaa gcagcgggag tactggaacg
     1621 cgttcgtcgg caagacggtg gaggagctga agtcgcacaa cgtgaagttc aacgatgtgg
     1681 atatcgctcc cttcgtccag tccgtgcagc cggtgtggga ggactatacg aagaagttcg
     1741 gaacggagct gctcgacctt attacgtcgg ctgcgaagta acgggacgtt cttttttccg
     1801 gaggagggcg gcgcgccgcg ccccttcctc cgttcaacga aaggcgggaa cctggcatgg
     1861 cgagatggac aacggataaa gagctgttct ctctgatgaa aaatgagctg tttacagcgc
     1921 ttgtgggcga tgtgctggac acgatggggc tgacgaagca gtttcttccg ccgcggatac
     1981 ggccgcttct gccggagacg gcgcttgccg gacgggcgat gacggtgctg gaggcggact
     2041 gttgcggggc gggccggaag cccttcgggt taatgttcga ggccctcgac gatctgaagg
     2101 aagacgaggt gtacatctgc gcgggtggct cgccggactt cgcgcactgg ggcggtctga
     2161 tgaccatccg ggcgaaacat ctgaatgcgg ccggagcggt gacgcacggc tatacgcgcg
     2221 atacgcggca ggtgctggag cttgattttc cggtgttctc gtggggaacg tacgctcagg
     2281 atcagggggc gcgcggacgc gtgatcgatt ggcgctgtcc ggtcgagttc gtcaacggcg
     2341 ttcgcgtgga gacgggagac cttctcttcg gcgatatggg cggcgtcgtg gtggttccgc
     2401 gcgccgtgga gagcgaagtc atcgagaagg cgctggagaa ggccgaggga gaaaatttgg
     2461 tggcgaaggc gatcatgggc ggcatgtcca ccgtggaggc ctttgcgacc tttggaataa
     2521 tgtagattct ggcccgaaag ggcggaggtg aatgctgtga gaagtgcaaa gctcgttgca
     2581 cccaggcaga ttgaaatttt tgaggacagg atgccccttc ccggcgaggg agaggtgctc
     2641 gtccgtgtga aggctgccgg gatctgcggt tccgacctgc atatctacca gggggagagg
     2701 gcggacgtga cgctcccccg gattcttggc cacgagctgg tgggcgaggt agagtcctcc
     2761 gggggcggcg cggaccgctt ctccaagggg gacagggtga cgatcgaccc tgtggtttcg
     2821 tgcggaagct gcgtttcgtg caaacggggg tacgacaacc tgtgcagcac ggtcaagtgt
     2881 ttgggggtgc aggttgacgg cgggtattgc gatttcatcg tcgttccgga ggagaaggtg
     2941 tacgcgctcc cgtcctctct gccgtgggag aaggcggcgc tgatagagcc cttctcgatc
     3001 gcggcggagg tgctttcacg aagcgaagtc cgtgaagggg acaaggtgct cgtccttgga
     3061 gccgggacaa tcggcctgtg cattctgcag gcgatgaagc tcgccggagc cgaggtgttg
     3121 acgagcgatt tcgtggagag ccggctggag aaggcgaagg agctaggcgc cgacagggtt
     3181 gtgaacagca agaccggcga tctttcagcg gcgctgcggg agttcaccgg agagttcggc
     3241 gccgatgtgg tggtcgaggc cgtgggcata ccccggcttc tggaggagag ccttggctac
     3301 gccgcgccgg gagggcggat cgtggtgctc ggcttcaacc cggagcctgc gaagatcccc
     3361 gaggtgctcg ttgtgaagaa agagctgaag atcatcgggt cgcgcatgaa ctgccggaag
     3421 tttcctcagg tgatcgagtg gttcgagaag gggttggtga acccggagcc gcttatctcc
     3481 gcgaagtacc ccttcgagaa gatcaacgac gcgttcaagg atattctgcg tgatccgcag
     3541 aagtacctta aggtgcttat cacctactga taagccggtt actcattttt tccagagagg
     3601 aaggcgaatg aacagatgat tctggatctt tttaagctgg acggcaagac cgccattgtc
     3661 accggagcgc gcaaggggtt gggctacggc atcgcggagg ggcttgccga ggccggggcg
     3721 aacatcgtcg gcgtgggacc gatcgcgatg cccgagatcg aggcgagagt gaaggccctc
     3781 ggacggaagt tcctccatgt ggaggcggat ctgacgtccc aggcgcgcat ccccgac
//
LOCUS       DAOPKU010000274         3831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_26353_length_3831_cov_3.835011, whole genome shotgun sequence.
ACCESSION   DAOPKU010000274 DAOPKU010000000
VERSION     DAOPKU010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26353_length_3831_cov_3.835011"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PK849_09095"
     CDS             complement(<1..1339)
                     /locus_tag="PK849_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPE66320.1"
                     /translation="MKRRNRFSHRMFLFLLCLLFLSFGSAEAATWTVTRTLDDGSTGT
                     LSHAVTHAAAGDSIVFAGNVTTVDLTSTLYLKKALTISGPATIRQTGANERVLYVTAD
                     CTFNYLTITGGNFVPGTQEGNYWNGAGIYNYANLTMNNCTVTGNTVGGGVYNVKNLTM
                     TGCTISNNTCAGSRHAGGINNEAKSSSAPVLTMYNCVVEGNSNPDTYGGGVFNGEKLF
                     MYGCTVRSNSSRGSGGGIYLNLFSETTLAQGCVVTGNSPDQICGPGQYGSLSIDGSCT
                     VGNTAGNKAVALSGFAQGSDPELRKTTGEPDVVAAEGALNDSGSGLFTSVKNTLHIDL
                     GASGEVVAGLASMSATLYNAFTYENVQLADASGEGELVIEFTASWPERVRYYAAFAEY
                     EGVAAGTLAVKGYVVAERGVQFETKPGQPLPDGVTPPDFYEEGEGLMTWRNVVA"
     gene            complement(1415..1558)
                     /locus_tag="PK849_09100"
     CDS             complement(1415..1558)
                     /locus_tag="PK849_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66321.1"
                     /translation="MEKKPLKNEGKIQEKDTKKKKKVDEMEESDLNGVAGGEGMDTVI
                     IKP"
     gene            complement(1770..3443)
                     /locus_tag="PK849_09105"
     CDS             complement(1770..3443)
                     /locus_tag="PK849_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPE66322.1"
                     /translation="MHMGRSVKLLRYFAVSGAALLMFWSVPAVEAKTWTVTTTADSRM
                     KEGSLRYIVAKANGGDTILFSSGGANLTSEIVLDKSLALVGPATIKQTGWERVFSIPA
                     GTTVTMENLTITGGSADYYGGGIYNEGMLTMTGCTVSDNRSARDGGGVYSGVYDYSTQ
                     TRTGTLTLTNCTVRNNAATFSGGGVFNGATMTSNGCAFSGNSAYWGGGLLMESNSHAS
                     ISEGVIENNAATDGGGLFNKGYVQNIQGTSFKRNNAEGKGGAIYGSNGILTVTACAIS
                     ANSAGSGGGGIYNTYIPGGRPIELKSGVLVTNNTPDQVNGYYVSDGSCIIGDAAGSKS
                     VALSGFAQGSAPKPRKTSGESDVDAAEQALKDSGSDLFTLVKKSMNADLGASGEVSAA
                     LSGMNAKLYDAFAYENVPLADASGKGELVIEFTASWPERARYYAAFAECGDGAADALS
                     VKGYVIAERGVQFEVKPGQPLPDGVTPPDFYEEGEGLMTWRNVVADNGPYDHNPAVGV
                     VTFRVCSVRAEAIQQQNGGGGGCSVGGAGSAWVAALLFVPVFALARKTR"
BASE COUNT          704 a         1232 c         1021 g          874 t
ORIGIN      
        1 ccgccaccac gttccgccag gtcatcagcc cctcgccctc ctcgtagaag tcgggcggcg
       61 ttacgccgtc ggggagcggc tgccccggct tcgtctcgaa ctggacgccg cgctcggcaa
      121 cgacgtaccc cttcacggcg agagtaccag ccgccacgcc ctcgtactcg gcgaacgccg
      181 catagtagcg aacgcgctcc ggccacgagg cggtgaactc gatgaccagc tctccctcgc
      241 cagacgcgtc ggcaagctgc acgttctcat aggtgaaggc gttatacagc gtcgcgctca
      301 tgctcgcaag gccggcgaca acctcccccg aagcgccgag gtcgatatgc agcgtattct
      361 tcaccgacgt gaacaggccg ctcccggagt cgttcagcgc accctccgca gcgacgacgt
      421 caggttcgcc ggtcgtcttc cggagttcgg gatcgcttcc ctgcgcgaag ccggagagcg
      481 cgacggcctt gttccccgcc gtgtttccga cggtgcacga accgtcgata ctcaacgagc
      541 cgtactgccc cggcccgcag atctggtcgg gagagttccc cgtcaccacg cacccctggg
      601 ccagggtcgt ctcgctgaag agattgaggt atataccgcc tcccgacccc ctgctgctgt
      661 tgctccgcac cgtgcacccg tacatgaaga gtttctcccc gttgaagacg ccgcctccgt
      721 aagtatcggg gttcgagttc ccctccacga cgcagttgta catcgtcagt accggagcgc
      781 tggatgattt agcctcgttg ttgatgccgc ccgcgtgccg cgatcctgcg caggtgttgt
      841 tgctgatcgt gcagccggtc atggtcagat tcttcacatt gtacacgccc ccgccgacgg
      901 tgttgcccgt cacggtgcag ttgttcatcg tcaggttcgc gtagttgtat attcctgcgc
      961 cgttccagta atttccctcc tgtgttccag ggacgaagtt tccaccggta atggtcaggt
     1021 agttgaacgt gcagtcggcc gttacgtaga gcacgcgctc gttcgccccc gtctggcgga
     1081 tcgtcgcagg gccgcttatg gtgagcgcct tcttgagata aagcgtcgag gtcagatcga
     1141 ccgtcgtgac atttccagca aatacgatac tatcgcccgc agcggcgtgc gtcaccgcgt
     1201 ggctcagcgt ccctgtggaa ccgtcgtcga gcgttctggt cactgtccac gtcgcggcct
     1261 ccgcgctccc gaagctcaag aagagcaggc agagcagaaa gaggaacatt ctatgagaaa
     1321 agcggtttct tcgtttcacc gtcgatcacc ctttcgaaaa aagcttgaac agaaaaaggc
     1381 ggagggcgct gttccaaccc tccgtccaca aagatcaggg cttgatgatc accgtatcca
     1441 ttccttcgcc gccggcgact ccgttgagat cgctctcttc catttcgtcg accttcttct
     1501 ttttcttcgt atccttctcc tggatctttc cttcgttttt gagcggtttc ttttccatcc
     1561 cgaacacctc catcgttttg agtacattca ccgcaccgcg catcgcccga cgtttccgtc
     1621 tatagagaga tgataaacct tgacactatg gtaaagtcaa ggggcaagca tataaatctt
     1681 ctcttttttc tcaaaagtcg gtctgtacat ccgcacacag gctctgcaag cagaggatgt
     1741 ctctttgatg agtcgtttct ctcaaggttc tatctcgttt tcctcgcgag agcgaagact
     1801 ggaacgaaga gcagggcggc gacccacgca cttccggcgc cgccgacaga gcagcctccc
     1861 cctccgccgt tctgctgttg gatggcttcc gcgcggacgg aacagacgcg gaaggtcacg
     1921 acgcccacgg caggattgtg gtcgtacggg ccgttgtccg ccaccacgtt ccgccaggtc
     1981 atcagcccct cgccctcctc gtagaagtcg ggaggcgtta cgccgtcggg gagcggctgc
     2041 cccggcttga cctcgaactg cacgccccgc tcggcgatga cgtacccctt cacggagaga
     2101 gcgtccgcag ccccgtctcc gcactcggcg aaagccgcgt agtaccgggc gcgctccggc
     2161 cacgacgcgg tgaactcgat gacaagctct ccttttcccg acgcatccgc aagcggcacg
     2221 ttctcgtagg cgaaggcgtc gtacaacttc gcgttcattc cggaaagagc ggcggaaact
     2281 tctcccgatg cgcccagatc ggcgttcatg ctctttttga ccagcgtgaa caggtcgctt
     2341 ccggaatcct tcagcgcctg ctccgccgcg tcgacgtccg actccccgct cgtcttccgg
     2401 ggtttgggag cgcttccctg cgcgaagccg gagagcgcga cggatttgct tcccgcagca
     2461 tctcctatga tgcaggagcc gtcgctcacg tagtatccgt tgacctggtc cggcgtattg
     2521 ttcgtcacga ggacaccgct tttgagttcg atgggtcgcc cccccggaat atacgtattg
     2581 tatatgcctc ccccgcccga accggcgctg ttggcagaga tggcgcaggc cgtcaccgta
     2641 aggatgccgt tggatccgta gattgcgcct cctttcccct ccgcgttgtt tctcttgaag
     2701 gacgttccct gtatgttctg cacatacccc ttgttgaaga ggccgccgcc gtcggtagcg
     2761 gcgttgtttt cgattacgcc ctcgctgatc gaggcgtgcg agttgctctc catcaaaagt
     2821 ccgccgcccc agtaggcgga gttcccggaa aacgcgcacc cgttcgatgt catcgtcgcg
     2881 ccgttgaaca cgccgccccc ggaaaacgtc gcggcgttgt tccgcactgt gcagttcgtc
     2941 agggtcaacg tgcccgtcct ggtctgcgtg ctgtagtcat agacgccgct ataaacgcct
     3001 cctccatctc ttgcggagcg gttgtcggag accgtgcagc ccgtcatcgt cagcattcct
     3061 tcgttgtata ttcctccccc gtagtaatcg gcgctccctc cggtgatcgt caggttctcc
     3121 atcgtcaccg tcgtcccggc ggggatggag aagacacgct cccacccggt ctgtttgatc
     3181 gtcgccgggc ctacgagcgc gagactcttg tccaggacga tctccgaggt gaggttggct
     3241 ccgccgctgg agaagaggat cgtatcgcct ccattcgctt tcgcgacgat gtagcgaagc
     3301 gacccctcct tcatcctcga atcggccgta gtggtcaccg tccacgtttt cgcctcgact
     3361 gcgggcacgc tccaaaacat caggagcgcc gcccccgata cagcgaaata ccttaaaagt
     3421 tttaccgacc tgcccatatg cattgccgat ttcatctcct tgtccgccat gactcgtctc
     3481 atgaggcggg gtatgtcttt ctatcgcaga atcatgataa accttcacac cgtgtaagag
     3541 tcaaggggga aattaaaaaa tctgcttagt cttcccaaaa aaaacaaaaa aaattatgct
     3601 ccgaaagcgg acagaattcc cgctcccctt gatcgcctct acattcgcgt tcaagagatt
     3661 cttcggtgct ccgtagagag tatctatttt ttaatttctt tttcagccct ctggcgtaaa
     3721 taccccaagt ggtatatact acaaaagtga tagagtttat cctatcaaac atacgatcac
     3781 aaggagggca ttcctatggc gaagtttgtt ttttccgttc ccgacatctc c
//
LOCUS       DAOPKU010000275         3819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_26459_length_3819_cov_4.605739, whole genome shotgun sequence.
ACCESSION   DAOPKU010000275 DAOPKU010000000
VERSION     DAOPKU010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26459_length_3819_cov_4.605739"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PK849_09110"
     CDS             complement(<1..286)
                     /locus_tag="PK849_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66323.1"
                     /translation="MKMFGRREGKLDLNQILMLICLTILIITVAMPMIMIVYNTFFYG
                     FTFDWSMFTNVVMDPSNVDAMWNTIVIAFWVTVVGTIVGVFYAWLIGRTNI"
     gene            complement(283..1371)
                     /locus_tag="PK849_09115"
     CDS             complement(283..1371)
                     /locus_tag="PK849_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66324.1"
                     /translation="MAGVTFKNITISYDAKVIIDNLSLEVDDGECFAILGPSACGKTT
                     LMRALCGFKNLDSGQIYIGDKLVGTKERWKSVAPEKRNVGVVFQDYAVWPHYSVFENV
                     YYPLKKRKVPKDEAKKRALDAIEQVRMKGYEQRMPAQLSGGQQQRVALARALVSSNQL
                     MVLDEPISNLDANLREEMRFEIKELQRRTGITVIYITHDQEVALAIADRMAILDRNGY
                     IRQIGVPEDVYRNPKDSYIYKFLGVSNFIPVERRGQEYVLDGTEGKQAFPYEIPEALR
                     AERLFAACRPVDITVGGNGPVQGFVKHVVYLGNLFEYRVLLGGREIRVQQDSHEAFRA
                     GVPEEGRPCALSFRNLRFYQEDEGGVAS"
     gene            complement(1556..2542)
                     /locus_tag="PK849_09120"
     CDS             complement(1556..2542)
                     /locus_tag="PK849_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66325.1"
                     /translation="MKKRVFSLLAVLVLVALMVPAFASAKEVMLYSSMKDSQLSALKD
                     AFIAKYPDIRMDYYTAGTGSVMTKLATEQQAGGIRADVIWVGDPTNYISFKEQGLLYA
                     YESPEAATMPANFKDPDNCYVAGRVIMLGFVYNTSALSANDVPKTWKELAEFKGYTAM
                     TDPTFSGTTLYTVAGLVQHPDFGWDFLQKLKDKGMLLERGSSAVVTKVGSGEYDVSVG
                     VDYIARTMIAQGSPIAFAYPQDGIPIILSPIAIVKNTANIEEAKLLFDFILSLEGQGI
                     LMKEYSAPVRPELSLEGALSVEEAAKKILPVDDNRLVAEKDELLKKFDSIFK"
     gene            2879..3796
                     /locus_tag="PK849_09125"
     CDS             2879..3796
                     /locus_tag="PK849_09125"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HPE66326.1"
                     /translation="MKIAVFGIGGVGGIVGGALAKDHPDTYFYVRGDNLDAIRRNGLK
                     VESAVFGDFTVRPKLASDKASELGAMDVVFVSCKGYSLKAACEAISPMVRPDTVVIPL
                     LNGVVVSDLMEPLLSPCILADGTIRVFSRLEKPGHIVQSTEMCSIVFGMKDGSRPAVL
                     DRIAAVLNKAGLKTTVSENILVDSWSKYVMMCGNSVLFCYYDGPAGKVREDPQYRKVF
                     DAVTGELIAVAAAKGVVLPAETAEKVAAAFSNFPPDAMSSLYRDLSGGKPARETELDH
                     IIGRMVEMGRQTGVPTPYHTIAYERFAGK"
BASE COUNT          800 a         1175 c         1065 g          779 t
ORIGIN      
        1 ggatgttcgt ccggccgatc agccaggcgt aaaaaacccc gacgatggtc ccgaccaccg
       61 tgacccagaa cgcgatcacg atcgtgttcc acatagcgtc gacattcgag gggtccatga
      121 cgacattcgt aaacatactc cagtcgaacg taaaaccata gaaaaacgtg ttgtagacaa
      181 tcatgatcat gggcatagcc accgtgatga tcaaaatcgt caggcagatc agcatgagaa
      241 tctggttcag atcgagcttg ccctctctgc gaccgaacat cttcatgagg ctaccccccc
      301 ttcgtcttcc tggtagaagc gtaaattcct gaaggagagc gcgcacggac gcccttcctc
      361 cggcactccg gccctgaagg cttcatggga gtcctgctgc acgcgaatct cccgccctcc
      421 gaggagcacg cggtattcga agagattccc caggtacacc acgtgcttca cgaagccctg
      481 aaccggcccg ttgccgccca ccgtgatatc cacagggcgg cacgcggcga acagcctctc
      541 cgcacggagc gcttcgggaa tttcatacgg aaacgcctgc ttcccctccg taccgtcgag
      601 gacgtactcc tgtccccgcc gttcgaccgg gatgaagttg gagacgccca aaaacttgta
      661 gatatagctg tccttggggt tcctgtagac atcctccggt acgcctatct gccggatgta
      721 gccattccgg tccagaatgg ccatccggtc ggcgatggcc agagccacct cctgatcgtg
      781 ggtgatgtag atcaccgtga tgcccgtacg ccgctgcagc tccttgatct caaagcgcat
      841 ctcctcgcgc aggttcgcgt ccaggttgga gatgggctcg tcgagcacca tcagctgatt
      901 cgaagaaacc agcgcgcgcg ccaaagcgac ccgctgctgc tgcccccccg agagctgggc
      961 gggcatacgc tgttcgtacc ccttcatgcg cacctgctcg atcgcgtcca gcgctctctt
     1021 tttcgcctca tccttcggaa ccttccgctt cttcaggggg tagtagacgt tctcgaacac
     1081 gctgtagtgg ggccacaccg catagtcctg aaaaaccacc ccgacgttgc gcttctccgg
     1141 ggcgacggac ttccaccgtt ccttggttcc gaccagcttg tcgccgatgt agatctgccc
     1201 cgaatccaga ttcttgaagc cgcaaagcgc ccgcatcagc gtggtcttcc cgcaggcgga
     1261 cggaccgagg atggcaaagc actccccgtc gtcgacctcc agcgacaagt tgtcgatgat
     1321 gacctttgca tcgtagctga tggtgatgtt tttaaatgtt actccggcca ttacgttcac
     1381 ctcgcactgc tttccaaaaa atgcatcgaa aagcgggtat aaaaaagcgg ccggacaatc
     1441 tccgggagaa cgcctgaaag gccgcttccc aaatattcct ccggccggcg acgccgcttc
     1501 gatggtactg aaggggaagg gcgtttcagg cccctccccg cgaaactctg tcgacctact
     1561 tgaaaatgct gtcgaacttc ttcagcagct cgtccttctc cgccacaagg cgattgtcgt
     1621 ccacgggaag aatcttcttc gccgcctctt ccacggagag ggccccttcg aggctcagct
     1681 ccggacggac gggggcgctg tactccttca tcaggattcc ctgaccttca agggagagga
     1741 tgaagtcgaa gagcagcttc gcttcctcaa tgttcgcggt gttcttgacg atcgcgatgg
     1801 ggctcaggat gatgggaatc ccgtcctgag gataggcgaa cgcgatggga gagccctggg
     1861 ctatcatggt gcgggcgata tagtccacgc ccacgctcac gtcgtactcg ccggaaccga
     1921 ccttcgtcac caccgcgctg gagcctctct caagcagcat acctttgtcc ttcagcttct
     1981 gcaggaagtc ccaaccgaaa tcgggatgct gcaccagtcc ggcgaccgtg tagagcgttg
     2041 tgccggagaa ggtcgggtcg gtcatcgcgg tgtacccctt gaactccgcg agctccttcc
     2101 aggtcttggg cacgtcgttg gcggaaagcg ccgacgtgtt gtacacaaag cccagcatga
     2161 ttacacgtcc cgcaacgtag cagttgtccg gatccttgaa gttcgcgggc atggttgccg
     2221 cctcgggaga ctcgtacgcg tacagaagcc cctgctcttt gaaggagata tagttcgtcg
     2281 gatctcccac ccagatgacg tcggcccgga tgcctccggc ctgctgctcg gtggccagct
     2341 tcgtcatcac gctgccggta cccgcggtat agtagtccat acggatatcc ggatacttcg
     2401 cgataaaggc gtctttcaac gcggacagct gagagtcctt catcgaagag tacagcatca
     2461 cctctttcgc cgaagcgaac gcgggaacca tcagcgcaac aagtacaaga accgccagaa
     2521 gactaaaaac acgctttttc attgtattgc cccctttgtg tataaaatgc ttggcaacgc
     2581 ggcaaagagc ccttttcgac cccttgcgta catgcgtgcg ttgccctgtt acagaattgt
     2641 aacattcaca cgccccgttt gccagtgttg ctggagtatt gatgcaacgg aggtcctttc
     2701 gccgccgtcc gcggcgtcgc tcacacatgc ttttcccggc tccgtgcgtc gtgttgctcc
     2761 cgtatggaaa ctgatagggc gcggtcccga tttgatccga ggaagaaaac ggcatatact
     2821 gaaagccgga aaagcgcgct tcacgcgctt actcgcagaa cttgggaggt gccatactat
     2881 gaaaatcgca gtgttcggaa taggcggggt cggcggaatc gtcggcggcg cgctggcgaa
     2941 agaccatccc gatacctatt tctacgttcg cggagacaac ctcgacgcca ttcgccggaa
     3001 cggactgaaa gtggaatccg ctgtcttcgg cgattttacc gttcgcccga aactcgcctc
     3061 ggacaaggct tcggagctcg gcgccatgga cgtcgttttt gtttcctgca aggggtacag
     3121 cctgaaagcg gcctgcgagg cgatctcccc gatggtacgg ccggataccg tcgtgatccc
     3181 tctgctcaac ggagtcgtcg tctccgacct gatggagccg ctgctgtccc cctgcatcct
     3241 cgccgacggg acgatccgcg tcttcagccg tctggagaag ccggggcaca tcgtccaaag
     3301 cacggaaatg tgcagcatcg tcttcggcat gaaggacgga agcagacccg ccgtattgga
     3361 ccgaatcgcg gctgtactca acaaggctgg cttaaagacg acggtgtcgg agaatatact
     3421 ggtggacagc tggtccaagt acgtcatgat gtgcggcaac agcgtgctgt tctgctacta
     3481 cgacggcccg gcgggcaagg tgcgcgagga cccgcagtat cgaaaagtgt tcgacgccgt
     3541 aacgggcgaa ctgatcgcgg tggccgccgc caagggggtc gtcctgcccg cggaaacggc
     3601 ggaaaaagtc gcggccgctt tttctaattt tccgccggac gccatgtcct ccctctaccg
     3661 cgacctcagc ggcggcaaac cggcgcgcga aaccgaactc gaccacatca tcggaagaat
     3721 ggtggaaatg ggacggcaga caggcgtgcc gaccccgtac cacaccatcg cctacgaacg
     3781 cttcgccggg aagtagcgat gcgaaacaat gatcgaaga
//
LOCUS       DAOPKU010000276         3773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_26911_length_3773_cov_3.684239, whole genome shotgun sequence.
ACCESSION   DAOPKU010000276 DAOPKU010000000
VERSION     DAOPKU010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26911_length_3773_cov_3.684239"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PK849_09130"
     CDS             <1..590
                     /locus_tag="PK849_09130"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPE66327.1"
                     /translation="AIAVSTYNAAMGRIVAAPTAGSCGILPGILFSYRDHFRCDDETL
                     LSGLFAAAAVGSVTAARATLAGASGGCQAECGAAAAMGAAALVQMRGGSPEAVGHAVA
                     LAYKSILGLVCDPVGGLVESPCIKRNGMLVSLSALAADMALAGLRSLIPPDEVIDAMG
                     QVGRALPESLRETAGGGLAVTPTARELVAKLLQQE"
     gene            complement(646..1734)
                     /locus_tag="PK849_09135"
     CDS             complement(646..1734)
                     /locus_tag="PK849_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPE66328.1"
                     /translation="MKIRNIRLGSLSVPLKKPFKTALRTVSEVTTNVVVVETEEGVCG
                     YGEAPPTAVITGDTNGAIRGAVEERLRPLLSGRETDDLNSLLDIVDTALVHNASAKAA
                     VDIALHDLWGKTLGRPLYELLGGTAREVETDYTISVNAPETMVKDSLEAVADGYSALK
                     IKVGTDFHLDMQRLEEIRAAVGKHVLLRVDANQGWTPKEAIRTIRFLEDKGLDIELVE
                     QPVKAHDFEGLKMVTDSVDTLILADESVFSVRDAFTVLSMRAADLINIKLMKTGGIRN
                     AVAICGMARACGVECMIGAMMETKISVSAACHLASALPVITRADLDPPILCASDPVVG
                     GATYRGSSVVPSGGAGLGIEKINGLVLE"
     gene            complement(1736..2932)
                     /locus_tag="PK849_09140"
     CDS             complement(1736..2932)
                     /locus_tag="PK849_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPE66329.1"
                     /translation="MITSGFAYVAFLFCFAGVVATLEMKYKDTTFFKYVPTPVVLYVM
                     CMIFATFDLWEKTDSVNAAYRVVRGNLIPAMIFVMLLRCDIRKILKLGPRMLIGFFSA
                     TITIMIGFVVMFALMKNGFPPESWKTWGALAGSWIGGTANMVAIQGALGISDSAMGYT
                     LLVDNVNYSIWVILLLATVPYAKFFNKWTKTDTSTIDSVGLMLSEVKENTRTKMETAD
                     LALLIGAGLAVSALSAWLASFISPMLMDVFGKSDFLSVSTVTIIIATIFGILCAMTPM
                     NSIPGSSPVSMTMLYIIICLIASRASFKELTDAPYYLAAGFVILGIHALLMAIIAKIF
                     KIDLFTCAVASLANIGAVAAAPIIAAAYKETLVPIGVLMALMGYVCGTAGGLFVAKLL
                     SMMAGA"
     gene            complement(3094..3444)
                     /locus_tag="PK849_09145"
     CDS             complement(3094..3444)
                     /locus_tag="PK849_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HPE66330.1"
                     /translation="MVHDAEYPKDSILVVGNSQTTGFNPINQQFGQFFITFILLKETE
                     EILDCAVSVTLETTGRFIRGLFVGRRLVLDESSIISDVQNRYFGSSQKAIIAAYRDAI
                     KKYKEVTALKKTMK"
     gene            complement(3481..3705)
                     /locus_tag="PK849_09150"
     CDS             complement(3481..3705)
                     /locus_tag="PK849_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPE66331.1"
                     /translation="MPVRLHLLGIFKKYQPSDPWEVQPGDQSVELLLKEAGMDSHAGY
                     VLLVNGSRKKKDYVPQEGDEIKVMPLVAGG"
BASE COUNT          797 a         1163 c         1070 g          743 t
ORIGIN      
        1 tcgccatcgc cgtctccacg tacaacgcgg cgatgggacg catcgtcgcc gctccgaccg
       61 ccggaagctg cggtattctg ccggggatac tcttctcgta ccgggatcat ttccgctgcg
      121 acgacgaaac gctcctctcc gggcttttcg ccgcagctgc ggtcggttca gtgactgctg
      181 cgcgcgcaac gctcgcggga gcctccggag gctgtcaggc cgagtgcggt gcggcggccg
      241 cgatgggagc agccgccttg gtgcagatgc gcggcggctc tcccgaagcc gtcggacatg
      301 ccgtcgccct cgcctataag tcgattctcg ggcttgtctg cgacccggtc ggcggactcg
      361 tcgaatcccc ctgcatcaag cgaaacggaa tgctcgtctc gctatctgct ctggccgcgg
      421 acatggcgct tgccggccta cgctctctca tccccccgga cgaagtcatc gacgcgatgg
      481 ggcaggtcgg acgcgccctc ccggaatcgc tgcgcgaaac cgcgggaggc ggcctcgcgg
      541 tgaccccgac cgcgagggag cttgtcgcca agctcttgca gcaggaataa caaaagcggg
      601 gcgaaccgga caattccggc gcgccccgct tctgttcgtt tccgattact ccaacaccag
      661 accgttgatc ttctcgatgc cgagccccgc tccgccggag gggaccaccg acgaacctct
      721 gtacgtcgcg ccgcccacaa cagggtcgga cgcgcaaaga atcggaggat cgagatccgc
      781 ccttgtgatc accggcagcg ccgaagcaag gtggcacgcc gccgatacgc tgatcttcgt
      841 ctccatcatc gcgccgatca tgcactccac gccgcaggcg cgggccatgc cgcagattgc
      901 gacggcattg cggatacccc cggtcttcat cagcttgatg ttgatcaggt ccgcagccct
      961 catagagagc accgtgaagg cgtcccgcac ggagaagacg gactcgtcgg cgaggatgag
     1021 cgtatcgacg ctgtccgtta ccattttgag cccttcgaaa tcgtgcgctt tcactggctg
     1081 ttccacaagc tcgatgtcga gccccttatc ctcaaggaag cggatggtgc gtatcgcctc
     1141 cttgggagtc cacccctggt tggcgtccac ccggagcagc acatgttttc ccacagccgc
     1201 cctgatctcc tcaagacgct gcatatcgag atgaaaatcc gtccccacct tgatcttgag
     1261 cgccgagtag ccgtccgcaa ccgcttccag gctgtctttc accatcgttt ccggcgcgtt
     1321 cacactgatg gtatagtccg tttccacttc cctggccgtg ccgccgagga gttcgtacaa
     1381 cgggcggccc agagtcttcc cccagagatc gtggagcgcg atatccacgg ccgctttcgc
     1441 ggaggcgttg tggacaagag ctgtatcgac aatgtcaagc agactgttca gatcgtccgt
     1501 ctcccgcccg gagagcaacg gacgaagccg ctcctcaacc gcgccgcgga tggcgccgtt
     1561 cgtatcgccg gtgatcaccg ccgtgggggg cgcttctcca tatccgcaga ctccctcctc
     1621 ggtctccacc acaacaacat tggtggtcac ttccgagacc gtccgaagcg ccgttttgaa
     1681 cggcttcttc agcggtacgg agagagagcc gagacggatg ttccgtatct tcattctacg
     1741 cccccgccat catggagagg agcttggcga caaagagacc gcccgcagtg ccgcacacgt
     1801 atcccatgag cgccatcagc acgccgatcg gcacgagcgt ctctttatat gccgccgcaa
     1861 tgatgggcgc cgcagcgacc gcgccgatat tcgcaagact ggcaacggcg caggtaaaga
     1921 ggtctatctt gaaaatcttc gcgatgatcg ccataagcag cgcatggatt ccgagaatga
     1981 cgaaccccgc ggcaaggtag tacggcgcgt cggtcagctc cttgaaggac gccctggagg
     2041 cgatgagaca gatgatgatg tagagcatgg tcatggacac aggggacgac ccgggaatgc
     2101 tgttcatcgg cgtcatggcg cagagaatac cgaagatggt cgcgatgata atggtgacgg
     2161 tgctcaccga gaggaagtcg ctcttgccga agacgtccat aagcatgggg gagatgaagg
     2221 aggctagcca ggccgaaaga gcggaaactg caagaccggc gccgataagg agggcaaggt
     2281 cggccgtctc catcttcgtc ctcgtgtttt ccttcacctc ggagagcatg aggccgacgg
     2341 aatcgattgt ggaggtgtcg gtcttcgtcc acttgttgaa gaacttggca tagggaacgg
     2401 tggccagaag aaggatgacc cagatcgagt agttcacgtt atccacgagc agcgtgtacc
     2461 ccatggccga gtcgctgatg ccgagagctc cctgaatggc caccatgttc gcggtgccgc
     2521 cgatccagct tccggcgagc gcgccccatg tcttccagga ctcgggaggg aagccgttct
     2581 tcatcagggc gaacatgacc acgaagccta tcatgatggt aatcgtcgcc gagaagaagc
     2641 cgatgagcat gcggggccca agcttcagta tcttccggat gtcgcagcga agcagcatga
     2701 cgaagatcat cgccggaatc aggttgcccc gcactacgcg gtacgcggcg ttgacgctgt
     2761 cggtcttctc ccacaggtcg aatgtggcga agatcatgca catgacgtag agaacaacgg
     2821 gcgtaggcac atacttgaag aacgtcgtat ccttgtactt catctccagg gtagccacta
     2881 cccccgcaaa acaaaaaaga aacgcaacat aagcaaaacc actcgttatc acagtaaaac
     2941 ccctcctaag aaaataaaag tatgatgcct tggggctgcg ccccatagaa aaaatccagc
     3001 agccgccgac gtatttctcg tcggtgaccg ctggatttca tcccctgttg tccgcgaggc
     3061 gaactcgctc ggagtcaagg acaaagcagc tcgttatttc attgttttct tcagagccgt
     3121 aacttccttg tactttttga tagcatccct gtaggcggcg atgatcgcct tctgggacga
     3181 gccgaaatac ctgttctgca cgtcggagat gatactgctt tcgtcaagga caagacgccg
     3241 cccgacaaac aacccccgaa tgaaccgccc tgtagtctcc agcgttacgg aaactgcgca
     3301 gtcgagaatc tcctcggttt ccttcagcag aatgaaggta ataaaaaatt gaccgaactg
     3361 ctggttgata gggttgaaac cggtcgtttg cgaatttccc accacaagga tggagtcctt
     3421 cggatactcc gcatcatgca ccatgtcgca attctatcat gacgccgaca gacttgtcta
     3481 tcatcccccg gcgacgagcg gcataacttt tatttcatcg ccctcctgag gaacgtagtc
     3541 cttcttcttc cgtgaaccgt tcacaagaag aacatacccc gcgtgcgagt ccattcccgc
     3601 ctctttgagc agcagctcca ccgattgatc gcccggctgg acctcccagg gatcggaggg
     3661 ctggtacttt ttaaagatgc cgaggaggtg cagccggacc ggcatggcta cttccccagc
     3721 gccatgaagg agagcatgag gccgccgcag ccgttggcca aatctccttc aaa
//
LOCUS       DAOPKU010000277         3769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_26949_length_3769_cov_4.735595, whole genome shotgun sequence.
ACCESSION   DAOPKU010000277 DAOPKU010000000
VERSION     DAOPKU010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26949_length_3769_cov_4.735595"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="PK849_09155"
     CDS             <1..1216
                     /locus_tag="PK849_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPE66332.1"
                     /translation="LFALKFADMKILLSDVFTEESRVKFHQNVQERLMRVAPFLYFDR
                     DPYLVVSEGRLVWMQDAYTAADSYPYAQPVSLGRGQNINYIRNSVKATVDAFDGTMRF
                     YAADPDDPVLKAWSGIFPEIFKPYSEMPKGLKAHLRYPQDLFNIQSEIYRTYHMTDAN
                     TFYNKEDVWDRPGSADSSGSAADAYYVNMRLAGEKHAEFALISPFMPVSRNNMIAWMA
                     GRSDGDNYGQLLVYQFPKQKLIYGPAQVEALTNQNSEISAQLSLWSQRGSSVVRGNMT
                     VVPVGNGLLYVQPLYLRAENSELPELRRVITSTGGRVVWGETLEESLVRLLSPTASEL
                     ETSSLVPAETTERTEFSGSEALAELAAEAMTHWDASQEALRKGDWEGYGRGMSGLENV
                     LREMMDRKDREQ"
     gene            1346..2083
                     /locus_tag="PK849_09160"
     CDS             1346..2083
                     /locus_tag="PK849_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPE66333.1"
                     /translation="MSLLIRRSITALFLFSALLFLLIGIEGEKIRFMIADMEIFRPPN
                     LGSTEGFWSVNPEMIELFQNAGRTIPSSFSDIARTFTSAGHVAATLFEATAENIRNLA
                     VFITEQNANILMESALIQAAAFWKYSIKYEVPLDLIVAVAHTESHFNPEARSGAGAAG
                     VMQVMWKVHSGLLQANGILSEEELHNPDLGIAAGSLLLSRYLRTHEDLQTALGRYFGG
                     PASVYWKKVSRNLEKLRKANIVAPSDI"
     gene            complement(2119..2490)
                     /locus_tag="PK849_09165"
     CDS             complement(2119..2490)
                     /locus_tag="PK849_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HPE66334.1"
                     /translation="MDRLSLFLLAGSAFFNALANTMMKFAFGHQKSLLDGGVVSAVLS
                     IVLNPWAVAGVGCFGVSFVFLSAALTRTDLSIAYPFMSGLVFLLILAVSTLFFSEQVN
                     VWRLAGMGSILAGIWMIAVKG"
     gene            2651..>3769
                     /locus_tag="PK849_09170"
     CDS             2651..>3769
                     /locus_tag="PK849_09170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPE66335.1"
                     /translation="MKLSESRSLRKYALLLILASFLLYFGGLGGYGLLEPDEGRYSEI
                     PREMLETGDFVTPRLNYVKYFEKPVLHYWLTAASLAVFGENEFAGRFWPAILALGSVL
                     ATWILARRLYGFPAAFFSSLILATSLLHFAIGRINIIDMPLSFFLTAAMAGLWFGIDE
                     TTEEETAKNRRYLLVFYAGMALAVLSKGLIGIVLPGGILFLYILLTRRWSLVRRVLYI
                     PGILLFFALTVPWFVEVCRRNSDFFYFFFIQEHFLRYATTMHGRYAPMWFFIPILIVG
                     FFPWAGLLPGAIRSALPLPLRTLGREKRNDLFLILWFGVIFVFFSISSSKLIPYIVPA
                     APPLAILMGRYLVRCIDEENAKKTGRFLLWNSLLLFPFI"
BASE COUNT          811 a         1134 c         1044 g          780 t
ORIGIN      
        1 tctcttcgcg ctgaagttcg ccgatatgaa gatccttctc tccgacgtct tcacagagga
       61 gagccgcgta aagttccacc agaacgtcca ggaacgcctc atgcgcgtcg cgccgttcct
      121 ctacttcgac agggatccgt accttgtcgt ctcggaagga cgcctcgtct ggatgcagga
      181 cgcctatact gccgcggaca gctaccccta cgcgcagccg gtctcgctcg gacggggaca
      241 gaacatcaac tacatacgga acagcgtaaa agccacagtc gacgccttcg acggcacgat
      301 gcgcttctac gccgccgacc ccgacgaccc ggtgctgaag gcgtggagcg gcatcttccc
      361 ggagatattc aaaccgtact cggaaatgcc gaaggggctc aaagcgcatc tgcgctatcc
      421 gcaggacctc ttcaacatcc agagcgagat ctaccgcacg taccacatga cggacgcgaa
      481 cactttctac aacaaagagg acgtgtggga caggcccgga agcgccgatt caagcgggtc
      541 ggcagctgat gcatactacg tgaacatgcg gctcgcagga gagaaacatg cggagttcgc
      601 cctcatttct ccgttcatgc cggtgagcag gaacaacatg atcgcgtgga tggcgggccg
      661 atcggacggg gacaactacg gccagttact tgtctaccag ttcccgaagc agaagctcat
      721 ctacggcccc gcgcaggtag aagcgctcac gaaccagaat tcggagatct ccgcacagct
      781 ctccctctgg agccaacgag gctcgtccgt cgtcaggggc aacatgacgg tggtacctgt
      841 ggggaacgga cttctctacg tccagccgct ctacctcagg gccgagaaca gcgaacttcc
      901 cgagctacgg cgcgtgatca catcgacagg aggccgggtg gtctggggag aaacgctcga
      961 ggaatcgctc gttcgcctgc tctctcccac ggcgtcggaa ctggaaacat cctcccttgt
     1021 tccggctgaa acaacggagc ggacggagtt ctcaggatcg gaagcgctcg ccgaacttgc
     1081 agccgaagcg atgacgcact gggacgcctc tcaggaagca ttgcggaaag gcgactggga
     1141 ggggtacggc agggggatga gcggactaga aaacgtactc cgtgagatga tggaccggaa
     1201 agaccgggag cagtagctca gctggcgccg tattgtaaag aaaatacacg cattcagact
     1261 cttgagagtg ctataataat gcgaccttcc atcggccctt cctttggaag gtcgcatttc
     1321 tttgaataag agggagggga acgaaatgtc gctcttgata cggagaagca tcaccgcact
     1381 ttttcttttt tcggcattgc tctttcttct cattggaata gaaggggaaa aaatccggtt
     1441 catgatcgcg gacatggaga tattcagacc gcccaacctt gggagtactg aaggtttctg
     1501 gtcagtcaac cccgagatga tcgagctctt tcaaaatgcc ggacggacaa ttccatcgtc
     1561 gttctccgat atcgccagga cattcacctc ggcagggcac gtcgccgcca cgctcttcga
     1621 agcgacagcc gaaaacatac ggaatcttgc cgttttcatc acggaacaga acgcgaacat
     1681 tctcatggaa tccgcactga tacaggccgc agccttttgg aaatactcca ttaaatacga
     1741 ggttcccctc gatctgatcg tcgccgtcgc gcacacggag agccacttca acccggaagc
     1801 ccgtagcggc gccggcgcag cgggcgtcat gcaggtgatg tggaaggtgc actccggcct
     1861 gctgcaagcc aacgggattc tctccgagga ggagctccac aaccccgact tgggaatagc
     1921 ggcgggcagc cttctcctct cacgctacct gcggacgcac gaggacctgc aaaccgccct
     1981 cggacgctac ttcggcggtc cggcgtccgt ctactggaag aaagtgtctc gtaacctcga
     2041 aaaactccgc aaggcgaata tcgtcgcgcc gagcgacatc tgagagagca cgaaggaagg
     2101 gaatccgcgc gttcgcattt accctttgac ggcgatcatc cagattccgg cgaggatcga
     2161 acccattccg gcaagcctcc agacgttcac ctgttcggag aagaaaagcg tggagacggc
     2221 gagaatgagc agaaagacca accccgacat gaacgggtag gctatggaga gatccgtcct
     2281 ggtaagcgcg gcgctcagaa agacgaagga gacgccgaaa cagccgaccc ccgctacggc
     2341 ccaagggttg agcacgatgg agagcactgc cgagacaact cccccgtcga gaagactttt
     2401 ctgatgcccg aaggcgaact tcatcatcgt gttcgccagg gcgttgaaga acgcagatcc
     2461 tgcaagaaga aacaacgaaa gcctgtccat agagaagtat ccaccctttc acatttcccc
     2521 ctccggctgc cggcggggta tccggtcacg tttcactccg aagaagtata tactctcccg
     2581 cgcactacca caaataaggg aacgtcatgt acaatgatgc gattggatct ttttttggaa
     2641 gggtggttcg atgaaactct ccgagtcgcg ttcgttacga aagtatgcgc ttcttctcat
     2701 tttggcctcg tttcttctct attttggcgg tctcggaggc tacggccttc tggaaccgga
     2761 cgaggggagg tactccgaga taccccgcga gatgctggag accggggatt tcgtcactcc
     2821 ccggctgaac tacgtcaagt acttcgaaaa acccgtgctc cactactggc tgaccgcggc
     2881 gtccctcgcc gttttcggcg agaacgagtt cgccgggcga ttctggcccg cgatcctcgc
     2941 actcggcagc gtcctcgcaa cgtggattct ggcgcggcgt ctgtacggtt tcccggcggc
     3001 attcttctct tcgctcatcc tggcgaccag cctgctccac ttcgccatcg ggcgaatcaa
     3061 catcatcgac atgccgctct cgttcttcct gacggcggcg atggccggcc tctggttcgg
     3121 aatcgatgag acgacggaag aggaaacggc gaaaaacagg aggtatctcc tggtctttta
     3181 cgcgggaatg gcgctcgccg tcctctcgaa agggctcatc ggcatcgtcc tcccgggcgg
     3241 catcctcttc ttgtatattc tgctcacacg ccgctggagc cttgtccgcc gggtgctcta
     3301 cattcccgga atcctccttt tcttcgcgct caccgtcccc tggttcgtcg aggtctgccg
     3361 cagaaacagc gacttcttct acttcttttt catccaggaa cacttcctgc gctacgcgac
     3421 gacgatgcac ggaaggtacg cgcccatgtg gtttttcatc cccattctga tcgtcggttt
     3481 ctttccctgg gcggggctgc tccccggcgc gattcgctcg gctttgcctc tgcccctgcg
     3541 aaccctcggc cgcgaaaagc ggaacgacct ctttctcatc ctctggttcg gcgtgatctt
     3601 cgtcttcttc tctatttcga gttcgaagct gatcccgtat atcgtgcccg ccgcgccgcc
     3661 gctcgcgatc ctgatggggc ggtatctcgt ccgctgcatc gatgaagaaa acgcgaagaa
     3721 gacggggcgc ttcctgctgt ggaattcgct gttgctgttt ccctttatc
//
LOCUS       DAOPKU010000278         3764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27001_length_3764_cov_5.207334, whole genome shotgun sequence.
ACCESSION   DAOPKU010000278 DAOPKU010000000
VERSION     DAOPKU010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27001_length_3764_cov_5.207334"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PK849_09175"
     CDS             complement(<1..294)
                     /locus_tag="PK849_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HPE66336.1"
                     /translation="MAQMTYMEALRDGMRMEMRRDKNVFLAGEDVGFFGGCFGQTAGL
                     FDEFGKERVVDTPITETAILGLGVGSAAAGLRPIVELMFMDFMGVCTDEINNQA"
     gene            complement(327..1289)
                     /locus_tag="PK849_09180"
     CDS             complement(327..1289)
                     /locus_tag="PK849_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HPE66337.1"
                     /translation="MEISKETLLGMYRKMLTIRRFEERAAELYAAGRMPGFVHLYVGE
                     EAVATGVCASLSDKDFITSTHRGHGHVIAKGGKTDLMMAELYGKVTGYCKGKGGSMHI
                     ADVDLGILGANGIVGAGQPISVGAAFACKYKKTDAVAVCFFGDGASNRGTFHEAMNMA
                     SSFKLPVVFVCENNMYGISNYQKHHMNISDIADRAGAYGVPGVSVDGNDVIAVYEAAS
                     EAVARARRGDGPSIVECKTWRHRGHFEGDPGKYKDPEEQKAWLAKDPIPRIEAKLIEL
                     GMATQADIDAMRAKVDEETEAAIKFAEDSPEPGDDELLTDVWAG"
     gene            complement(1586..2605)
                     /locus_tag="PK849_09185"
     CDS             complement(1586..2605)
                     /locus_tag="PK849_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPE66338.1"
                     /translation="MEKREYTGVLIFVQQTDGEVAPVSLELLGKGRELAATLGTEVTA
                     ALLGSGIDPLLPLLARYGAERIVVVDDPALETYMTEPYTRAMAAVTERFKPEILLCGA
                     TAVGRDLAPRLSARLHTGLTADCTGLEIDPQTRNLMMTRPAFGGNIMATIVCAEHRPQ
                     MATVRPGVMQRIEPLKSSQPFVERMRVEGIAEGRNVEILEVVKKMSAKKDIQEARVLV
                     AGGRGMGGPENFAVLEELAEVLGGTVASSRASVDAGWVGKDRQVGQTGKTVRPDLYIA
                     CGISGAIQHLAGMEESGIIVAINKDPSAPIFEAADYGVVGDALKIVPMLAESIRNARA
                     RKAEV"
     gene            complement(2618..3400)
                     /locus_tag="PK849_09190"
     CDS             complement(2618..3400)
                     /locus_tag="PK849_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPE66339.1"
                     /translation="MKILVCIKQVPDTTEVRLDPATGTLIREGIPSIINPDDKAGLEA
                     ALRLKDEHGAHVAVLSMGPPQTDLALREALAMGADEAVLLTDRAFGGADTWATSSTLA
                     SAISGMEYDLIITGRQAIDGDTAQVGPQIAEHLGIPNISYAEELRVEDGYVVARRQYE
                     DRSHMLKVRMPCLITALSELNEPRYMTPRGIFEAFREREVKLLTRADLAIDDGNIGLK
                     GSPTRVMKSFTKAAKGKGTVVDLDARSAADWITERLQEKFVL"
     gene            complement(3412..>3764)
                     /locus_tag="PK849_09195"
     CDS             complement(3412..>3764)
                     /locus_tag="PK849_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPE66340.1"
                     /translation="YVKERKQFGKPIGAFQNTQFRMADMKTRIEAARLLVYSAALAKD
                     SGGRFSVEAAMAKLFASETAMAVTTKCVQLYGGYGYMRDYPVERMMRDAKITEIYEGT
                     SEVQRMVVAGALLK"
BASE COUNT          602 a         1327 c         1099 g          736 t
ORIGIN      
        1 cgcctggttg ttgatctcgt ccgtacagac gcccatgaag tccatgaaca tcagctcgac
       61 gatgggacgg agtcctgccg ccgcggaacc gacccccagt cccagtatcg ccgtctccgt
      121 gatgggggta tccaccacgc gctccttgcc gaactcgtcg aagagccccg ccgtctggcc
      181 gaaacacccg ccgaagaatc ccacatcctc gccggcgaga aagacgttct tgtcccggcg
      241 catctccatc ctcattccgt ccctgagcgc ttccatatat gtcatttgtg ccatcttcgc
      301 cactctcctt cggtcatccg cgccgctcaa ccggcccaca cgtcggtgag aagttcgtcg
      361 tcgcccggtt cggggctgtc ttcggcgaac tttatggccg cctcggtctc ctcgtcgacc
      421 ttcgccctca tcgcgtcgat atcagcctgc gtcgccatgc ccagctcgat cagcttggcc
      481 tcgatgcgcg ggatcgggtc cttcgcgagc cacgccttct gctcctcggg atccttgtac
      541 ttcccggggt cgccctcgaa gtggccgcga tggcgccacg tcttgcactc gacgatgctc
      601 ggaccgtcgc cccggcgtgc gcgcgccaca gcttcggagg cagcctcgta gacggcgatc
      661 acgtcgttgc cgtccacgct gacccccggc acgccgtacg ctcctgcgcg gtcggcgatg
      721 tcgctgatgt tcatgtggtg cttctggtag ttggagatgc cgtacatatt gttctcgcag
      781 acgaagacga ccggcagctt gaaagaggac gccatgttca tcgcctcgtg gaaggtaccg
      841 cggttggagg cgccgtcgcc gaaaaagcag accgctaccg cgtccgtctt tttgtacttg
      901 caggcgaacg ccgccccgac ggagatcggc tgcccggccc cgacgattcc gttggccccg
      961 aggatgccga ggtcgacgtc cgcaatgtgc atcgagccgc ccttcccctt gcagtacccc
     1021 gtcaccttgc cgtacagctc cgccatcatc agatcggtct tcccgccctt ggcgatcaca
     1081 tgcccgtgcc ccctgtgggt gctcgtgatg aaatccttgt cgctcaaaga ggcgcacacg
     1141 ccagtcgcca cagcctcctc acccacgtaa aggtggacaa accccggcat cctccccgcc
     1201 gcgtacagct ccgcggccct ctcctcgaac ctcctgatgg tcagcatctt ccggtacatc
     1261 ccgagcaacg tttccttact gatctccata cccgttttcc ctccttgcac aaaaaaactt
     1321 aatttccagg cttcactgta aacacagcct tatcggaatt atcatatacg ctaagacctg
     1381 tcaaggattt tcacgggagg agaagaaacg tatactccgt acaaaacttt tcttttccat
     1441 acgcagagcg gccctcttca cgagagtgaa aagagccgcc ggggatgaag cataattacg
     1501 ctcttcgcaa gaaagagacg ggaagcgtcc gtggaggagc gtccggaggt ccgaagcgcc
     1561 cgcgcaacgg agagactgcc gcgcgctaca cctccgcctt ccgggcgcgc gcgttccgga
     1621 tgctctcggc gagcatcggg acgatcttca gcgcatcgcc gaccacgccg tagtcggccg
     1681 cctcgaagat gggagccgag gggtccttgt tgatggcgac gatgatcccg gactcctcca
     1741 ttccggcgag gtgctggatg gcgccggaga tgccgcaggc gatgtagagg tccggacgga
     1801 cagtctttcc cgtctgcccc acctgccggt ccttcccgac ccagcccgcg tcgacgctcg
     1861 cccgcgagga ggcgaccgtt ccgccgagga cctccgccag ctcttcgaga acggcgaagt
     1921 tctcgggtcc gcccattccg cgccctccgg cgacgagcac ccgggcctcc tgtatgtcct
     1981 ttttcgcgct catcttcttc acgacttcga gaatctccac attccgcccc tccgcgatcc
     2041 cttcgacgcg catccgttcc acaaagggct gagagctctt cagcggctcg atcctctgca
     2101 tcacgccggg acggacggtc gccatctgcg gacggtgctc cgcgcagacg atggtcgcca
     2161 tgatgttgcc gccgaaggcc ggacgcgtca tcataaggtt ccgcgtctgc gggtcgatct
     2221 ccagccctgt gcagtcggcg gtgagaccgg tgtgcagccg cgcggagaga cgaggcgcga
     2281 ggtcgcgtcc gacggcggtg gcgccgcaga gcaggatctc gggcttgaac cgctccgtca
     2341 cggcggccat cgcccgcgtg tacggctcgg tcatgtaggt ctccagcgcc gggtcgtcca
     2401 ccacgacgat acgctccgcg ccgtagcgcg ccaagagcgg gaggagcgga tcgatcccgg
     2461 agccgaggag cgccgcggtc acctccgtcc ccagcgtcgc cgccagctcc cgccccttgc
     2521 ccagcagctc cagcgagacg ggagcgacct ccccgtccgt ctgctggacg aagatcagaa
     2581 cgcccgtgta ttcacgcttt tccatagtac agcctcctca cagcacgaac ttctcctgca
     2641 acctctccgt gatccagtcc gccgcgctcc tcgcgtccag gtcgaccacg gttcccttcc
     2701 ccttcgccgc cttggtgaac gacttcatca cacgcgtggg ggagcccttc agcccgatgt
     2761 ttccgtcgtc gatcgcaagg tcggcgcggg tcaggagctt cacttccctc tccctgaagg
     2821 cctcgaagat gccgcggggc gtcatgtatc ggggctcgtt cagctccgag agcgcggtga
     2881 tcagacaggg catcctcacc ttgagcatgt ggctccggtc ctcgtactgc cgccgcgcca
     2941 ccacgtaccc gtcctccacc cgcaactcct cggcgtagct gatgtttggt atgccgaggt
     3001 gctccgcgat ctgcggcccc acctgcgccg tgtcgccgtc gatagcctga cggcccgtaa
     3061 tgatcaggtc gtactccatg ccggagatgg cggaggcgag cgtcgaggag gtcgcccagg
     3121 tgtccgcgcc gccgaacgcc ctgtcggtga gcaggacggc ctcgtccgcg cccatggcga
     3181 gcgcttcgcg gagcgcgaga tccgtctgcg gcggccccat gctgagcacg gcgacgtgcg
     3241 ctccgtgctc gtccttcagg cggagcgcgg cttcgagccc cgccttgtcg tccgggttga
     3301 tgatgctcgg gatcccctcc cggatcagcg tgccggtcgc cgggtcgagg cgcacctccg
     3361 tggtgtcggg cacctgtttg atgcacacga gaatcttcat ggtcttcctc cctacttcag
     3421 cagcgcgccg gcgacgacca tgcgctgcac ttcgctggtg ccttcgtaga tctcggtgat
     3481 cttggcgtcg cgcatcatcc gctccacggg gtagtcgcgc atgtagccgt aaccgccgta
     3541 aagctggacg cacttcgtgg tcaccgccat cgcggtctcg gaggcgaaga gcttggccat
     3601 ggccgcctcg acggagaatc gtccgcccga gtccttggca agcgccgcgc tgtagaccaa
     3661 cagtctggcg gcctcgatcc gcgtcttcat gtccgccatc cggaactgcg tgttctggaa
     3721 ggcgccgatc ggcttgccga actgttttcg ttccttgacg tagg
//
LOCUS       DAOPKU010000279         3761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27031_length_3761_cov_4.763896, whole genome shotgun sequence.
ACCESSION   DAOPKU010000279 DAOPKU010000000
VERSION     DAOPKU010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27031_length_3761_cov_4.763896"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PK849_09200"
     CDS             complement(<1..1350)
                     /locus_tag="PK849_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE66341.1"
                     /translation="MYYWNGKASKGEISREDAQKMAGDVIFMLRYEGDNYIFSYDMEN
                     KVSIPFQSHERGKFLDVKDQDGNWVQRDLREIAKTKGKGFYTYNWLNSNTKRVEPKVA
                     YVRYFEPFDWWYGTGVYVEDIKAKALRSTMVQAGILGVSILLICISIALLTRRFITAP
                     LRRVVLLSERAGSGDLTVNRNDFAYSGKDEIGLMADALSSMISNQAKTVRGIVGTVSE
                     VSSAAENLSALSEETEASLEEMQKFLAQVGEMTESGAAAAEEANAGVAEVAEGAQSVS
                     RSATTGAEAGSQADSIVKGTVDQFESVIANVARVEKQGEANMQAVSSLASSVQEITGF
                     VVTISRIADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEESNEAAKAIRVLIN
                     GLEEKAGASVQSIESSDKILKETSALSQRSMDELRKGMGAVQRVISVISDLAAVSEEQ
                     "
     gene            complement(1397..2599)
                     /locus_tag="PK849_09205"
     CDS             complement(1397..2599)
                     /locus_tag="PK849_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE66342.1"
                     /translation="MNISSIIEKANALTEKITELRRMFHAHPELPWLETETSLFVEQY
                     LRKLGLENIRRGFDGTQSGVMADLKGGKPGRCVALRADIDALPLEEENDLPYKSTYSG
                     VMHACGHDAHTAVLLGVAEILSSMKDELCGTVRFLFQPAEEAGMHSGAPKMIEEGALD
                     GVEAIGGLHVWALLESGKLGYRIGPVMASADIWDLKIQGKGGHGAMPHHAVDPTVTAA
                     TVISTLQTVVSREIDPQETVVLSIGKLEAGTAVNIIPDTARVAGNVRTTNPEIRARMQ
                     GIMKRVADGICAAMRCTAELVYTPIYPVTVNDPAMTQLMRSVAVEVFGEERVVELPVA
                     MGSEDFSYYGEKIPAAYVFLGIADEAKGTGNQHHNPKFNVDDEPLPKGAALLAGFAAR
                     FTAGESAS"
     gene            complement(2717..3490)
                     /locus_tag="PK849_09210"
     CDS             complement(2717..3490)
                     /locus_tag="PK849_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPE66343.1"
                     /translation="MRKVFLNEFSSSELERMIGAGEIDSAITIFGSCESHGPHLPLGP
                     DLFVPTEIARRAALRLARTVVVPGVPFGTSLHYNRFPLSVTLRFETTIAVAEDILTSL
                     IENGIRRIVILNGHDGNIPALEIAARKVKDRFPDATLVYLPAWWNITGAKMADDFEVW
                     NGLGHGGEGETSIMMAARPDLVDLTQAKSQVPHDVIALSDFATVIWDIAEITSTGATG
                     DSTKATQEKGEKMLRCVTDYLVGLVEELERRGWKYDLKK"
     gene            complement(3487..>3761)
                     /locus_tag="PK849_09215"
     CDS             complement(3487..>3761)
                     /locus_tag="PK849_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66344.1"
                     /translation="FFVKKGRYIAENLENEPAVRMTGVLSFLAGGSLGLFFQYGAPLP
                     YGLPSGLFALAATIVLYVLLYRRTQDAAYDSALFAAADRKAQEVRS"
BASE COUNT          737 a         1239 c          988 g          797 t
ORIGIN      
        1 ctgctcctcg gaaaccgctg caaggtcgga tatgacgctt atcacgcgct gcacggcgcc
       61 cattcctttt cgaagctcat ccatggatcg ttgagagagc gccgacgtct ccttgaggat
      121 cttgtcggag gactcgatcg actggacgga tgcgcccgcc ttctcctcca gaccgttgat
      181 gagcacgcgt atcgccttcg cagcttcgtt ggactcctct gcaagcttcc ggacttcatc
      241 ggccactacg gcaaaccctc ttccggcctc cccggccctc gcggcttcaa tagccgcgtt
      301 cagggcgagc agattcgtct ggtcggctat acgcgaaatc gtcacaacaa aacccgtgat
      361 ctcctggacc gacgaggcaa gcgacgaaac agcctgcata ttcgcctcgc cctgcttttc
      421 caccctggcc acatttgcga taacggattc aaactgatca acggttccct tcacaatgga
      481 atcagcctga ctccccgcct ccgctccggt tgtcgccgac ctggaaacag actgcgcccc
      541 ctccgcgact tccgcaacgc ccgcgtttgc ctcttccgcc gcggcagccc ccgactccgt
      601 catttctccg acctgtgcaa gaaacttctg catctcctca agagacgcct cggtctcttc
      661 gctcagcgcc gagagattct ccgcggcgga cgaaacttcc gacaccgtgc cgactattcc
      721 tctgactgtc ttcgcctgat ttgatatcat ggacgataac gcgtccgcca tcaaccctat
      781 ctcgtccttc cccgaatacg cgaagtcgtt cctgttcaca gtgaggtcac cgctccccgc
      841 ccgttcggag agaagcacca ctcttcgcag gggagcggta atgaagcgcc gcgtaagaag
      901 cgctattgaa atgcagatca aaagaatgga cacgccgagg attccggcct gcaccatcgt
      961 actgcgcaag gctttcgcct tgatgtcctc cacgtacacg ccggtgccgt accaccagtc
     1021 gaatggctcg aaatagcgaa catacgccac cttgggctcg accctctttg tgttcgaatt
     1081 cagccagttg taggtataga atcctttccc ttttgtcttg gcgatctctc tcaggtcgcg
     1141 ctgcacccag ttcccgtcct ggtccttcac atcaaggaac ttgccccgtt catgactctg
     1201 gaacggtatg gacactttgt tctccatgtc gtagctaaaa atatagttgt cgccttcata
     1261 cctgagcatg aaaatcacat cgccggccat cttctgtgca tcttcgcggg aaatctctcc
     1321 tttggaggcc ttcccgttcc agtagtacag agtgaaggac tattctaaag aagaatgagc
     1381 acccggccgg gataggtcag gaagcggatt cacctgccgt aaatcttgcc gcgaaaccgg
     1441 caagcagagc ggcccccttc ggcaagggct cgtcatcgac attgaacttc gggttatggt
     1501 gctgattgcc ggtccccttc gcctcgtcgg cgatcccgag gaacacgtac gcggcgggaa
     1561 tcttctcccc gtagtaactg aagtcttcgg aacccatcgc gacgggaagc tccacaacgc
     1621 gctcctcgcc gaacacctcg acggcgacgg agcgcatgag ctgcgtcata gcgggatcgt
     1681 tcaccgtcac cgggtagatc ggggtataga caagttcggc cgtgcagcgc atcgcggcgc
     1741 agattccgtc ggctacgcgc ttcataatgc cctgcatccg ggcgcgtatc tccgggttcg
     1801 tcgtacggac gtttcccgcc acccgcgcag tgtccgggat gatgttcacg gcagttccgg
     1861 cttccagttt ccctatgctg agcacaacgg tctcctgcgg atcgatctct ctgctcacga
     1921 ctgtctgcag cgtcgagata acggtcgcgg cggttactgt cggatcgacg gcgtgatgcg
     1981 gcatcgcgcc gtgcccgccc ttcccctgaa tcttcagatc ccagatgtcc gcagaagcca
     2041 tgacaggacc gatgcgatac cccagcttgc ccgactccag aagcgcccac acgtgcagac
     2101 cgccgatggc ctctacaccg tcgagcgccc cttcctcgat catcttcggt gcgcccgagt
     2161 gcattccggc ctcctcggcg ggctgaaaga ggaaacggac cgtaccgcaa agctcgtcct
     2221 tcatcgagga caaaatctcg gcgacgccaa gcaagacagc agtatgcgcg tcgtgaccgc
     2281 aggcatgcat cactccgctg taggtcgact tataggggag atcattctct tcttccaagg
     2341 gaagcgcatc gatgtcggcc cgcagagcga cgcatctgcc cggcttcccg cccttcagat
     2401 cggccatgac gccggattgc gtcccgtcga atcctctcct gatattctca agccccagct
     2461 tccggagata ctgctccaca aagagcgacg tctccgtctc cagccagggg agctccggat
     2521 gcgcatggaa cattctgcgc agctccgtga tcttctccgt cagtgcgttc gccttttcaa
     2581 tgatggacga aatattcatg gcagagcgtt ctcccttccc ggttctcgaa cacaataata
     2641 cactgcggag aagtccattc cgggaaaacc cgaaagctcc tctctccggc aagcggacac
     2701 attcccgcag gggatctcat tttttcaggt cgtatttcca tcctcgacgc tccagctcct
     2761 ccaccaggcc gaccaggtag tccgtcacgc agcgaagcat tttttctccc ttttcctgcg
     2821 tcgctttggt ggagtccccc gttgcgccgg tggaggtgat ctcggcgata tcccagatga
     2881 ccgtcgcaaa atcgctcagc gcgatcacat cgtggggaac ctggctcttc gcctgtgtca
     2941 ggtcgacgag gtcggggcgc gccgccatca tgatggaggt ctccccctct cctccgtgtc
     3001 ccaggccgtt ccacacctcg aagtcatccg ccattttcgc gccggtgatg ttccaccatg
     3061 cgggcaggta gacgagcgtc gcgtcgggaa agcgatcctt gacctttctg gccgcgatct
     3121 ccagcgccgg gatgttgccg tcatgtccgt tcaggatgac gatccggcgg atgccgtttt
     3181 cgatcaggct cgtcaaaatg tcctccgcca ccgcaatcgt cgtttcgaaa cggagcgtca
     3241 ccgacagggg aaaccggtta tagtgcaacg acgtcccgaa cggtactccg ggaacgacaa
     3301 cggtgcgcgc cagccgaagc gccgcgcgtc gcgcaatctc cgtcgggacg aagagatccg
     3361 gaccgagcgg cagatgcgga ccgtggctct cgcagctgcc gaaaattgta attgcgctgt
     3421 cgatctcccc cgcgccgatc atccgctcca gttcggaaga ggaaaattcg ttcaaaaaca
     3481 cctttctcat gaccgcacct cctgcgcttt cctgtccgcc gccgcaaaga gcgccgagtc
     3541 gtatgcggcg tcctgcgttc tcctgtacag cagaacgtaa agaacgatcg tggctgcaag
     3601 cgcgaagagc ccggacggga gaccgtacgg caaaggcgcg ccgtactgga agaagagccc
     3661 caggcttccc ccggcaagga aggagagcac gcccgtcatt ctgaccgcgg gttcattctc
     3721 caggttctcc gcgatatacc gccccttttt gacgaagaac c
//
LOCUS       DAOPKU010000280         3745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27190_length_3745_cov_4.631165, whole genome shotgun sequence.
ACCESSION   DAOPKU010000280 DAOPKU010000000
VERSION     DAOPKU010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27190_length_3745_cov_4.631165"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="PK849_09220"
     CDS             complement(<1..1672)
                     /locus_tag="PK849_09220"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPE66345.1"
                     /translation="MLQVNEMNRNAMTIQLEGPLPDYPVFVEGMRRAPDRGWTLNRSE
                     TELALKNALRYVPEMYHKELIPEFLEELRTRGRIYAYRFRPEGRIKGLPVHQYKGKCL
                     AGKAFQVMIDNNLDFEIALYPYELVTYGETGQVCQNWLQYRLIKRYLEELTEETTLVV
                     ESGHPLGLFKSRPDAPRVILTNGMLVGLFDNPAEWHRGMQLGVTNYGQMTAGGWMYIG
                     PQGIVHGTFNSILNAARQRLGVGEKENLAGILFVSSGLGGMSGAQPKAVEIAGGVGII
                     AEVDASRIETRHSQGWVAKVTGSCKEAFDLAAEACATKQSLSIAYHGNIVDLLQYAAE
                     HDISIPLLSDQTSCHAPYDGGYCPQGLTFDERTKMLSKNPEEFRRLVDISLRKHYEFI
                     KTLVDRGTYFFDYGNSFMRAVFDAGVPEICKNGETTYDGFVWPSYVEDIMGPVLFDYG
                     YGPFRWVCLSGDPEDLRKTDHAAMECIDPDRRSQDYDNWLWIRDAEKNALVVGTQARI
                     LYQDAEGRTRIALKFNEMVRKGEIGPVMLGRDHHDVSGTDSPYRETANI"
     gene            complement(1704..2462)
                     /locus_tag="PK849_09225"
     CDS             complement(1704..2462)
                     /locus_tag="PK849_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPE66346.1"
                     /translation="MIKSEHDSKQDGAFKVTAIMEALCASSSSLTVREIEERTGIPRS
                     TAHRFLVSLEERRWVFSDAATEGYRPGIRFFHLSSKSSFYDELVKKAEPGMFRLMTST
                     GNTAVLSVAEGSSGSCIHSVEPLSPVKFTAHRGMTIPLHAGATGKILLAFCSQEVRST
                     VLSSSLRSPVDGEEIDKRAFEEELLRIREQGFASSREEWMPHAGDISVPVFDRRGEFI
                     AQMGVAGIAENIFRNFDENLRLLREAAHNLESML"
     gene            2688..>3745
                     /locus_tag="PK849_09230"
     CDS             2688..>3745
                     /locus_tag="PK849_09230"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HPE66347.1"
                     /translation="MLTANLIGPPRFFLDDRELFFPIRKAQALLCYLLVEERASREAL
                     SSLLWGGSEDMLASQSLRNALYLLRKGLPEGLVGTGRQWITVDMSRVNLDLLLLERLD
                     DAPLERLPALCRPLLEGFSLPNAPEFDDWLRTARFQWAGRIRSAFLALSRLRMEAGKW
                     EAALCPLNLVAAGDEIDEESYRLIMRAHAARREWGRVAEAWRVLEGRLKNELGVEPSS
                     ESLALAASLLKKRDTRTQVFEKTQPAPRKDFFFGRSSELRSVREFCGAVSASPRCIFV
                     EGEAGVGKSLFVKTALPLSFPAEPLILRCTAGNPEAAYPLLPWDELLSSLFRSVDRES
                     LFRSPVGGPRLLKDHPSL"
BASE COUNT          666 a         1072 c         1143 g          864 t
ORIGIN      
        1 tgatattcgc cgtttcgcga tagggggagt ctgtcccgga gacgtcgtga tggtcgcggc
       61 ccagcatcac cgggcctatt tctccctttc gcaccatttc gttgaatttg agggcgatgc
      121 gggtgcgtcc ctcggcgtcc tggtagagta tccgggcctg cgttcccacc acaagggcgt
      181 tcttctcggc atcgcgaatc cagagccagt tgtcatagtc ctgtgagcgc cggtctgggt
      241 cgatgcactc catcgccgcg tggtcggtct tgcgcagatc ctcagggtcg ccgctgaggc
      301 agacccagcg gaacggaccg tagccgtagt cgaagaggac gggacccatg atatcctcca
      361 cgtaagaggg ccatacaaag ccgtcgtacg tggtctcgcc gtttttgcag atctcgggga
      421 ctcccgcgtc gaagaccgcg cgcatgaacg agttgccgta gtcgaagaag tatgttcccc
      481 tgtcgacgag cgtcttgatg aactcgtagt gcttcctcag ggatatatcc acaaggcggc
      541 ggaactcctc cgggttcttc gagagcatct tggtgcgctc gtcgaaggtc agtccctgcg
      601 ggcaatagcc tccgtcgtag ggagcgtggc aggaggtctg gtcggagagc agcggaatgg
      661 aaatgtcgtg ttctgccgcg tactgcagca gatccacgat gttgccgtgg tacgcgatcg
      721 aaagcgactg ttttgtcgcg catgcctcgg cggcgagatc gaaggcttcc ttgcagcttc
      781 cggtgacctt ggctacccac ccttggctgt ggcgcgtctc gatgcgcgaa gcgtccacct
      841 cggcgatgat gccgacgccg cctgcgattt ccacggcctt cggctgcgcg ccgctcatgc
      901 cgccgagtcc ggaggagacg aagaggattc ccgcgagatt ttctttctca ccgacgccga
      961 gccgctgccg ggcggcgttg agaatgctgt tgaacgtacc gtggacgatg ccctgggggc
     1021 cgatgtacat ccaccctccg gccgtcatct gtccgtagtt ggtcacgccg agctgcatac
     1081 ccctgtgcca ctccgccggg ttgtcgaaga ggccgacgag catgccgttg gtcagaatga
     1141 cccggggcgc gtccggacgc gatttgaaaa ggccgagggg gtggccgctt tcgacgacga
     1201 gcgtcgtctc ctcggtcagc tcctcaaggt accttttgat caaacgatac tggagccagt
     1261 tctggcagac ctgaccggtc tcgccgtacg tgacgagttc gtacgggtag agggcgattt
     1321 cgaagtcgag gttgttgtcg atcatgacct ggaacgcctt cccggcgagg cacttcccct
     1381 tgtactgatg caccgggagg ccctttatcc gcccctcggg ccggaacctg taggcgtaga
     1441 tgcgccccct ggtacgaagc tcctccagga attcagggat cagctccttg tggtacattt
     1501 cgggcacgta gcggagggcg ttcttcaatg caagctccgt ctcgcttcgg ttgagcgtcc
     1561 acccccggtc gggcgcgcgc cgcatcccct cgacgaagac ggggtagtcg gggagagggc
     1621 cttccaactg gatggtcatg gcgttccggt tcatttcgtt tacctgcaac atctcttcca
     1681 cctccacaag gtcttggtac attttacagc attgattcca gattgtgcgc tgcctcccgc
     1741 agaagacgaa gattttcgtc gaaattgcgg aagatgttct cggcgattcc ggcaactccc
     1801 atctgcgcga tgaactctcc ccgtcggtcg aagaccggga cgctgatatc ccccgcgtgc
     1861 ggcatccact cctcgcggct ggatgcgaat ccctgctccc gtatgcgcag cagctcctct
     1921 tcgaaagctc ttttgtcgat ctcctctccg tcgacgggag atcgcaacga agaggaaagt
     1981 actgtggacc gcacctcttg agaacagaag gcgagtaata ttttccccgt ggctcctgcg
     2041 tgaagaggaa tcgtcatccc ccggtgtgcg gtgaacttta ccggcgagag aggctcgacc
     2101 gagtggatgc acgaccctga ggatccttcg gcgacgctca ggaccgcggt attgcccgtg
     2161 cttgtcataa gacggaacat ccctggctct gcttttttaa cgagttcgtc gtagaaagag
     2221 gatttgctgc tgagatgaaa gaaacggatg cccggccggt acccctcggt tgcagcgtcg
     2281 ctgaagaccc aacggcgttc ttcaagagag acgagaaacc tgtgggctgt gcttctgggt
     2341 attcctgtcc gttcctcgat ctcgcggaca gtgagcgacg aagaggatgc gcagagcgcc
     2401 tccatgattg ccgtcacttt gaatgccccg tcctgcttag aatcgtgctc gctttttatc
     2461 atcgcgacct ccaaaatggg acaatgtgtc ccattattat catgagcgtg ccggtttgtc
     2521 aatcgctgcg gcagggtccc gacaggggcg tgtgaggaga ggctccattc atcttttgag
     2581 ttcttgtttt tccggtgttc ctgggttccg ggaggaggtc gatggtataa tcgttcatag
     2641 tttttcagta acaatttcgt atttcccgag ggaaagggga gccgttcatg ctcaccgcca
     2701 acctgatagg ccctccgcgg ttttttcttg acgacagaga gctgttcttt ccgatccgga
     2761 aggcccaggc gctcctttgc tatctcctgg tcgaggaaag agcttctcgt gaggcgctct
     2821 cgtcgttgct ctggggcggg agcgaagaca tgcttgcttc ccagagcctg aggaatgcgc
     2881 tctaccttct tcggaaaggc ctccccgagg ggctcgtggg aacgggccgg cagtggatta
     2941 ccgtagatat gtcccgtgtg aaccttgacc tgcttctttt ggaacgcctt gacgacgccc
     3001 ctctcgagcg tcttcccgct ttatgccgtc cgctccttga gggattctcg ctgccgaacg
     3061 cgccggagtt cgatgactgg ctcaggactg cacgcttcca gtgggccgga agaattcgct
     3121 ccgcttttct tgcattgtct cggctgcgta tggaggcggg gaagtgggag gctgcgctgt
     3181 gtccgctgaa ccttgtagcg gcaggggacg aaattgacga agagtcgtac cgcctgataa
     3241 tgcgcgctca tgcggcgcgg cgcgagtggg gcagagtggc ggaggcgtgg agggtacttg
     3301 aaggccgcct gaagaacgag ttgggcgtgg agccgtcgag cgagagtctg gctctcgccg
     3361 cgagtcttct taaaaagcgg gatacacgaa cgcaggtatt tgaaaaaacg cagcctgctc
     3421 caaggaagga cttctttttt ggacgttcct cagagctgcg ctctgttcgg gaattttgcg
     3481 gtgcggtgag cgcgtcgccg cgatgcattt tcgtcgaagg agaggccggt gtcggcaaga
     3541 gtctcttcgt caagacggcg ctccctcttt ctttcccggc cgagccgtta atactgcgat
     3601 gtaccgctgg aaatccggag gctgcgtatc ccttgcttcc atgggacgaa ctcctgtcgt
     3661 ctctcttccg ttcagtcgac agagagagtc ttttccgttc gcccgtggga ggacctcgcc
     3721 tgctcaagga tcatccatca ctgca
//
LOCUS       DAOPKU010000281         3744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27207_length_3744_cov_3.764164, whole genome shotgun sequence.
ACCESSION   DAOPKU010000281 DAOPKU010000000
VERSION     DAOPKU010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27207_length_3744_cov_3.764164"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..1034)
                     /locus_tag="PK849_09235"
     CDS             complement(282..1034)
                     /locus_tag="PK849_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE66348.1"
                     /translation="MMKFLKAILVVVFCLVSAGAWALTADDVVYMTEEYPPFNMAGSD
                     GVATGASVEVMAGIFERMGASKTVKDIQVLPWARGYKEVQNTPNTCLFLMTMSDERKP
                     MFKWVGPVAPTKIGIMALKSKGLKIASVNDLVNLKAGVIREDIGDSLAQQAGIKHIER
                     VPTNEQNIKKISEGRIDIWAFEESVAKWQIKGFGFNPDDFESIWVLSESDLYFAFHKD
                     TDEALIAAMQKVLDEMKADGAYDAIMKKYAIN"
     gene            1479..3170
                     /locus_tag="PK849_09240"
     CDS             1479..3170
                     /locus_tag="PK849_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE66349.1"
                     /translation="MSKSIVAKLGLLVAVSMILLAGSLAFMAQRGVDQMSSSVERVAE
                     EMLNEDIQNKNANDERSAQAYGEAMSGYLAVISATPLWDFNEESLKDYAKDMLNVPNV
                     AYAVIYAQDGSKMAGEKKEGPAVRPFTAEILREGKKIGSVEIGLDTGYLTELTRASEK
                     TRDQLISSFSAQAAEAEETVVRRILLFALGIVTAVLILNVIGLLRVVAPLRRMTDVVR
                     DLGEGEGDLTVSIAVRTEDEVGRLGSSMNQFIEKLSHLVIDVIGISRHLGDGSRRLSA
                     LSGKSMESIELVNGAVEEIVSLSESNAAAVEQTNAGVAEMSATSVSVAQAAGQCVESS
                     AKTTGFTKDVARKLEEVVSDIEAVSARSQENRAKMSALAEAVDSIAGFVGAITGFADQ
                     TNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEESNRAASEISNLMSSLSGYAGESI
                     KATEEEEKTLRTVVEKAGILQQTLKASMDELSAMDAELNKVSELARDQSHSNIEIAGA
                     VDNIAQGTSNIVERLEEIRNSAQAAREAFESVTDQSRTLLQGMEEMERQLGQFKV"
     gene            3507..3680
                     /locus_tag="PK849_09245"
     CDS             3507..3680
                     /locus_tag="PK849_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HPE66350.1"
                     /translation="MSTKVQCIVCEGDVILPAEVLEGELLTCPDCGTELEIVSLNPVT
                     VAEAPEVQEDWGE"
BASE COUNT          872 a         1032 c         1002 g          838 t
ORIGIN      
        1 ccacgatgac ggcccgccgt tcctccgcgg gaagatattt tccgttcgtt gccatgaggc
       61 tgcaccttct tctttttata agatagtaat tacttactat cttatgtgtt tcccccctct
      121 ctgtcaagca cgactgatga tacacacaaa aaagcggcct ccccgaaaac cgtcatgggc
      181 agcagcatgt gccatgcgga tccggtactg ttacccaaag aaaaaggccg ccccggaaag
      241 ggcggcctcg gcacgttttg ttttggaaga gcactgaacg gtcagttgat ggcgtacttt
      301 ttcatgatgg catcataggc gccatcggcc ttcatttcgt cgagaacttt ctgcatcgcc
      361 gcaatcagtg cctcgtccgt gtccttgtgg aaggcgaagt aaaggtcgct ttcgctcagg
      421 acccagatgc tttcaaaatc gtcgggattg aatccgaatc cctttatctg ccatttggcg
      481 acgctctctt caaaggccca gatatcgatg cgcccctcgc tgatcttctt gatgttctgc
      541 tcgttggtgg gaacccgctc tatgtgcttt attcccgcct gctgggcaag cgagtccccg
      601 atatcctcac ggataacgcc tgctttcaga ttcacaaggt cgttgacgga tgctattttc
      661 aaccccttgc tcttgagagc cattatgccg atcttggtcg gggccaccgg accaacccat
      721 ttgaacatcg gtttacgctc gtccgacatg gtcatgagga aaaggcaggt attgggcgta
      781 ttctggactt ctttataccc ccgcgcccac ggaagcacct ggatgtcctt caccgttttc
      841 gaggcgccca ttctctcgaa aatgcccgcc attacttcaa cggaggctcc agtcgccact
      901 ccgtcggacc ccgccatgtt gaaggggggg tactcctcgg tcatgtacac gacatcatcg
      961 gccgtcaaag cccaggcgcc agcggaaacc aggcagaaga ccaccacaag tattgccttc
     1021 aggaacttca tcatctcgtc ctcctttgtt tgtcgccaga ctgttcaccc tgcattgcac
     1081 agcaataaac ggaacactat tttgagtaaa aaaacatctt attctataaa ttatactcta
     1141 tcccgattag aaatatatca aaccttcgaa ggaagtcaag gtcattcttc cggcgcgggc
     1201 tcagaaattt tctcgcatac gttcaccgcc gcatttcaat gaacctggca gtattcatgc
     1261 atacttttcc gcagagtgtc ttgcaataaa accgttttcg agctatccta cattaaggaa
     1321 tagttcataa tttcatcaga ttttccaaaa taaaatccgg tccggcaacc ccgctttctt
     1381 tcttgggaat tttttcatcg aatagcgctc ttctgaagac tttgttcagt gtcgacgggc
     1441 atcatccgcg gcatcatatc aatgaggggg tattgcacgt gtcgaaaagc atcgttgcaa
     1501 aactcggatt gctggtagcg gtcagcatga ttctccttgc gggctcgctg gcgtttatgg
     1561 cccagagagg agtggaccag atgtcttcct cggtcgagcg cgtcgctgaa gagatgctca
     1621 acgaggacat ccagaacaag aacgccaacg atgaacggag cgcccaggcc tacggagaag
     1681 ccatgtccgg atacctggcg gtcatttccg ccactccctt gtgggacttc aacgaagaat
     1741 cgctgaagga ctacgcgaag gacatgctca acgtgccgaa tgtggcctat gccgtcatct
     1801 acgcccagga cggctcgaaa atggccgggg aaaagaagga gggaccggca gtccggccct
     1861 tcactgccga aatactgagg gaaggaaaaa agataggatc ggtcgaaatc gggctcgata
     1921 cggggtatct cacagaactc acacgggcca gcgaaaaaac cagggatcag ctcatctcct
     1981 cattctcagc ccaggcagcc gaagcggagg aaaccgttgt acggaggatt ctcctcttcg
     2041 cccttggcat tgtgacggcg gtgctgatac tcaacgtcat cggacttctt cgggtggtcg
     2101 ccccgctgcg ccgcatgacc gacgtggtcc gtgacctcgg cgagggggag ggtgatctca
     2161 ctgtctccat cgccgtcagg acggaggatg aagtggggcg cctgggctct tccatgaacc
     2221 agttcatcga aaagctctct caccttgtta tcgacgtgat cggcatatca aggcatctcg
     2281 gagacggatc gcgcaggctt tccgcgctct ccgggaaatc catggaatcc atcgagcttg
     2341 tcaacggcgc ggtggaggaa atagtctcac tctccgagtc caacgcggcc gcggtcgaac
     2401 agacgaacgc cggcgtcgcg gagatgtcgg caacgtcagt ctccgttgca caagcggcag
     2461 gacagtgcgt ggagtcgtcg gcgaagacga caggatttac gaaagacgtc gcccgcaagc
     2521 tggaggaggt tgtctcggat atcgaggcgg tcagcgcccg ttcacaggaa aaccgggcaa
     2581 agatgtccgc cctggcggag gcggtcgatt cgattgcggg tttcgtcggc gccataacgg
     2641 gatttgctga ccagacgaac ctgctcgcgc tgaacgccgc catcgaggcg gcaagggccg
     2701 gagaggcggg acggggcttt gccgtcgtgg cagacgaagt ccggaagctc gccgaagaat
     2761 ccaaccgcgc tgcgagcgaa atttccaatc ttatgagctc gctctcggga tatgcgggag
     2821 agtccatcaa ggccacggag gaagaagaga aaacgctgcg caccgtggtc gaaaaggcgg
     2881 ggatcctcca gcagaccctg aaggcaagca tggatgaact ttccgccatg gacgcggagc
     2941 tgaacaaagt ctcggaactg gcccgcgacc agtcccattc gaatatcgaa attgcggggg
     3001 ctgtggacaa catcgcccag ggaacatcca atatcgttga acggctcgag gaaatcagga
     3061 attccgccca ggccgcccgt gaagcgttcg aatcggtgac ggatcaatcg cggacgctcc
     3121 tccaaggtat ggaggagatg gaacgccagc tcggtcagtt taaagtttaa ccgcttcccg
     3181 cctccggatg acgttcctcc tgcgggtcct gtacacgggg tgatttcggg gagtgaattt
     3241 ccgggaatga taatgaccgc aacggaggta ccgcacctga cgcgcggtac ctccggcttt
     3301 ttttgaatac ttccatccga agtcacgggc ggcattgtgt tcccctcata ttccaggagc
     3361 acaccaaaat caaccagcga cagtaattcc tgccgttttc cgtaagaaac atacagaaaa
     3421 aagaaacatt tatttcttac ttgacgaaaa aggaacacag gtttacaatc cccccattgt
     3481 aaatatttat tttggaggga ttctttatgt caactaaagt tcagtgtatt gtctgcgaag
     3541 gggacgtcat tcttcccgcc gaagttctgg agggcgagct tcttacatgc ccggactgcg
     3601 gcacggaact cgagatcgtt tctctgaacc cggtcaccgt ggccgaggcc cccgaagtac
     3661 aggaggactg gggagagtag atggcacatt tcttcatctt ctacacccgc ctccgcaccg
     3721 aagagaaact gcttttccgg gccg
//
LOCUS       DAOPKU010000282         3740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27243_length_3740_cov_4.908548, whole genome shotgun sequence.
ACCESSION   DAOPKU010000282 DAOPKU010000000
VERSION     DAOPKU010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27243_length_3740_cov_4.908548"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PK849_09250"
     CDS             <1..433
                     /locus_tag="PK849_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE66351.1"
                     /translation="AAQEVQKLIAGLQAGAKSSLAVTGEAEEIMKQTVSSAEEALTEL
                     RKAMDQIARTSDAVQGIAAAAEEQAASAQQMTAGVEQVTRSTSEVMDALHIIQSASGE
                     TVKASQDLAAEAQELLANVEKIEALLSDFTVVKEKAGLLPR"
     gene            610..1785
                     /locus_tag="PK849_09255"
     CDS             610..1785
                     /locus_tag="PK849_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPE66352.1"
                     /translation="MKLSMRKCLLIVCFLPFFISSASAAVVPFPELCASGTPAQIRAA
                     ILDGADIVERNSEGVTPLMFAAAGNPDPLAASELLDAGASLTERDYAGLTPLMWAAAR
                     NEEPQVSALLLQRGAEVNARSRDGVTPLLFAAGNNENPEIASLLLKAGADKDAKNRSG
                     WSAFMFAAANNPNPEILRVLRSSGADPNARSADGWTPLMRAAAHTSAPEIVREFLRFG
                     ADANARGGEQETPIMAAARFNTNAAVLRELLQNGADPNARDKNGVTVLMWAAANNLNP
                     EIVSVLAKAGAYPDARGKNGDTAIMGAAMSNPNPQVVAALVEAGADVNMKGPRDWTAL
                     MFGALHTRNPEVVSSLLAAGANATIQNRDGGRAIDYAGKNPHLKGTNAYWLLNEASY"
     gene            complement(1810..>3740)
                     /locus_tag="PK849_09260"
     CDS             complement(1810..>3740)
                     /locus_tag="PK849_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66353.1"
                     /translation="WKLFKYSLLSYLLLAGLFTMLYSLFAYSREKDEMERTLYTVRRT
                     HVPNIVDALWITDTQRVQRELDAIARFEFVDRAEVRDDEGRILFAGSEPLLEAQLRTE
                     TLRYAHDDRSVVIGELSVFLGVRRMALKIINNAMLLLLLQTALSVLLATIAALAFHAS
                     FGRPLSRFARFISADESSLSGEPFVLESRMHDRDELGILVSSFNRQHSATARSIAEIN
                     EMNRRLSDANALLRQSEEALRTSKESAEWLGRQAESANRAKSVFLANMSHELRTPLNS
                     VIGFLDLLGVTPLDEKQREYLGYVRTSAHSLLDVISNILDISKIETGKLELKPEFTNI
                     RRLLEQAMNIARGTAVEKGLHLVLRVEDATPRRIFVDAIRLKQVLVNLLGNAVKFTGK
                     GEVELSLRFEETGNETGSFLFSIRDTGIGIPKEKRERLFEPFYQGDESITRQYGGTGL
                     GLPICDALLQRMGSALELESVPGEGSRFFFTLAAGYADDEEIEEPFDASGHAPSDSVL
                     FAIHTPERPTVLIVEDQRLNRMLLKKLVDALLPSATVYEAVDGVEGVELFERHAPDIV
                     FMDLQMPRKDGFHAATEIRTLEADGRRTLIIALTADAQPETRESCLESGMDDYLTKPV
                     SIHDLRRVLEHGAMRTEE"
BASE COUNT          708 a         1080 c         1233 g          719 t
ORIGIN      
        1 tgcggcccag gaggtacaga agctcatcgc ggggcttcaa gcgggagcga agagctcgct
       61 cgccgtcacc ggcgaggccg aggagatcat gaagcagact gtctcttccg cggaggaggc
      121 cctaacggag ctccggaagg cgatggacca gatcgcgaga accagcgacg ccgtgcaggg
      181 gatcgccgcc gcggcggaag agcaggccgc gtctgcgcag cagatgacgg cgggcgtcga
      241 acaggtgacc cgttcgacga gcgaggtcat ggatgcgctg cacatcatcc agagcgcctc
      301 gggggagact gtgaaggcct ctcaggatct tgcggccgag gcgcaggagc ttctggcgaa
      361 cgttgaaaag atcgaggcgc tgctctcgga ttttaccgtc gtaaaggaaa aggcggggct
      421 tctgcctcgc tagtctcttt tcagaagcgc gtaacaggtc cggatatgag atacccgctt
      481 catacgcccg cagtcgcccg caaagcgatt cgcgggcgtt tttaatagcg cagtcgcgtg
      541 gaataaaagg actctttttt cggtggtatc atgttttcga tgcacatatg ttcgggagga
      601 gggagttgta tgaaattgag catgcggaaa tgcttgctga tcgtgtgttt tcttccgttc
      661 tttatttctt ctgcatccgc cgctgtcgtt ccgtttccgg agctgtgcgc ctccggaacg
      721 cccgcgcaga ttcgagcggc tatcctggac ggcgccgata tcgtcgagcg gaacagcgaa
      781 ggcgtgacgc cgctgatgtt cgcggccgcc ggtaatcctg acccgcttgc ggcctcggag
      841 ctgctggacg cgggagcgtc gctcaccgag cgggattacg cagggctgac gccgctcatg
      901 tgggcggcgg cgcggaacga ggaacctcaa gtttcggcgc tgctgttgca acggggcgcg
      961 gaggtgaacg ccagaagcag ggacggtgtg acgccgctgc tcttcgccgc ggggaacaac
     1021 gaaaatccgg agatcgcgtc gctcttgctg aaggcgggcg ccgacaagga cgcgaagaac
     1081 aggagcggat ggagcgcgtt catgttcgcg gcggcgaata atccgaaccc ggagatcctc
     1141 cgggtgttgc gcagcagcgg cgcggatccg aacgcgcgga gcgcggatgg atggactccc
     1201 ctgatgcggg cggcggcgca tacgtccgct ccggagatcg tccgcgaatt tttacgtttc
     1261 ggcgcggatg cgaacgcacg cggcggcgag caggaaacgc cgatcatggc cgcggcgcgt
     1321 ttcaacacca acgcggcggt tctccgcgag ttgcttcaga acggggcgga cccgaacgcc
     1381 agggacaaga acggcgtcac ggtgctgatg tgggctgctg cgaacaacct gaatccagaa
     1441 attgtgagtg tgttggcgaa agccggggcg tatccggacg cgagggggaa gaacggcgac
     1501 acggccatca tgggagcggc gatgagcaac ccgaacccgc aggtcgtcgc cgcgctcgtc
     1561 gaggccgggg cggacgtgaa tatgaaggga ccgcgcgatt ggaccgcgct gatgttcggg
     1621 gcgctgcaca cccggaaccc tgaagtggtg agctcgttgc tggcggcggg ggcgaacgcg
     1681 acgatacaaa acagggacgg cgggcgcgcc atcgactacg ccggaaaaaa tccgcacctc
     1741 aagggaacca atgcatactg gcttctgaac gaggcctcgt actgagaggc ctcgtactga
     1801 atcgaaggat tattcctccg tcctcatcgc tccatgttcc aagactctcc ggagatcgtg
     1861 gatgctcacc ggcttggtga ggtaatcgtc cattcctgat tcgaggcagc tctctcttgt
     1921 ctccggctgg gcgtcggcag tgagcgcgat gatcaatgtt ctccgtccgt ccgcttccag
     1981 agtccgtatc tccgtcgcgg catggaaacc gtcctttcgg ggcatctgca gatccataaa
     2041 gacgatgtcc ggagcgtggc gttcgaagag ctccactccc tccaccccgt cgacggcctc
     2101 gtagacggtt gccgacggca gcaacgcgtc gaccagtttt ttcaacagca tccggttgag
     2161 cctctggtct tcgacgatga gcaccgtagg ccgctccggg gtgtgaatcg cgaagaggac
     2221 gctgtcggag ggggcgtgtc ccgaagcgtc gaagggttct tcgatctcct cgtcgtcggc
     2281 gtatccggcg gcgagcgtga agaagaagcg gcttccctct ccgggcacgc tctccagttc
     2341 aagcgcgctt cccatcctct gcaggagcgc gtcgcagatg ggaagtccca gccccgtccc
     2401 tccgtactgt cgtgtgatcg attcatcccc ctgatagaag ggctcgaaga gccgctcccg
     2461 tttttccttc gggataccga tgccggtgtc ccggatggag aagaggaagc ttcccgtttc
     2521 gttgcccgtc tcctcgaagc ggagcgagag ctccacctcc cctttcccgg tgaacttgac
     2581 cgcgttgccc agaaggttga ccagcacctg cttcaagcga atcgcgtcga cgaagattcg
     2641 gcggggcgtt gcgtcctcga cccggagcac gagatggagg cccttctcta cggcggtacc
     2701 ccgtgcgatg ttcatcgcct gttcgagaag acgccggata ttcgtgaatt caggcttcag
     2761 ttcgagcttg cccgtttcga tcttcgaaat gtcgaggatg ttgctgataa cgtcgaggag
     2821 ggagtgtgct gacgtcctga cgtaccccag gtattcgcgc tgtttctcgt cgaggggggt
     2881 gacgccgagc agatcgagga agccgatcac gctgttcagc ggtgtgcgca gttcgtggct
     2941 catattcgcg aggaagacgc ttttcgccct gttcgcgctc tcggcctgtc tgccgagcca
     3001 ctcggcgctc tccttggacg tccgcagcgc ctcctccgac tgacggagga gtgcgttggc
     3061 gtcgctcagc cgcctgttca tctcgttgat ttcggcgatg gagcgggccg tcgcgctgtg
     3121 ctgccgattg aagctcgaca cgaggatccc cagttcgtcc ctgtcgtgca tgcgactctc
     3181 cagaacgaac ggttctcccg acagagaact ttcatccgcg gagatgaagc gcgcgaagcg
     3241 ggaaagcggc ctcccgaagg aggcgtgaaa cgccagcgcg gcgatggtcg cgagcagaac
     3301 ggaaagagct gtttgcagca gcagaaggag cattgcgttg ttgatgatct tcagcgccat
     3361 tctgcggacg ccgagaaaga cggagagttc gccgatgacg acggagcgat cgtcgtgtgc
     3421 ataccgaagc gtctcggtcc ggagctgcgc ttcaagaagg ggctccgatc cggcgaagag
     3481 gatgcgcccc tcgtcgtccc gtacctcggc cctgtcgacg aactcgaagc gcgcgatcgc
     3541 gtcgagttcg cgctgcactc gctgcgtatc cgtgatccag agtgcgtcga cgatgttggg
     3601 aacgtgcgtc cggcgtactg tatagagcgt ccgctccatc tcgtcctttt cgcgcgagta
     3661 tgcaaagaga gagtagagca tcgtgaagag ccccgcgagc agaaggtacg agagtaagga
     3721 atatttaaac agcttccacg
//
LOCUS       DAOPKU010000283         3735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27300_length_3735_cov_4.012500, whole genome shotgun sequence.
ACCESSION   DAOPKU010000283 DAOPKU010000000
VERSION     DAOPKU010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27300_length_3735_cov_4.012500"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PK849_09265"
     CDS             complement(<1..1244)
                     /locus_tag="PK849_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPE66354.1"
                     /translation="MDILAGYAGKVLRVDLSSGAVSTELLTEEKLRPFLGGTGYAARI
                     LYDELKAGTDPLGPDNLFVLATGPLTDNLVPGGGSLSFCCKSPLTGGWAEARVGCDMG
                     GTLKKAGYDFVVLSGRAERPVHLVITDGRAELESAEHLKGRTVLEKNALLNEGGYSEY
                     DSLVIGPGGERLVLFASVMVGHRAAGRVGCGAVLGSKNVLAVSVRGSGKVVPADAAGY
                     MKAVREAHRIVKESPNTYGFTELGTMGGYEACDAMGDLPTKNWQSNSWGKGAQIASHF
                     VEENFTGGTGCYKGCPMKCGRKVRVKDGKWKTPEHDGGEYETIGSFTAYMLNEDVDCA
                     VHCGYLCNELGIDTISAGGAIGFLMECAEKGLLSPEMTQGLDLSWGNASVLPKLVRMI
                     ALREGVGDILADGVRRAAERIGG"
     gene            complement(1384..1842)
                     /locus_tag="PK849_09270"
     CDS             complement(1384..1842)
                     /locus_tag="PK849_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPE66355.1"
                     /translation="MKKVVVPIDGSDTSKGVVDYAVYYANREHDANMLFLHVITVMEH
                     EPVFYGEGEVVLPPSDEKVTAEFTKFIEDRMEAAGMRIPRMSVSVRSGKVYEQIVRFA
                     EEQDADMIMIGHRGLSPMQRFFLGSVAAKVVAQAPCSVYVHRPKERRGKA"
     gene            complement(2035..>3735)
                     /locus_tag="PK849_09275"
     CDS             complement(2035..>3735)
                     /locus_tag="PK849_09275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE66356.1"
                     /translation="CFVVEVSSAEEASRLLSEGGVALFVADALFASPRETAEILRSLL
                     AVRPVPLLLVFPSSGEEIADELAVFAQSGFVTQETPESLLRVAIHNSRRLFEIRMCPP
                     VKRTIGQCAACGLRERCELRMSEERFRSLVANIPGVVFRCRSDPEWTMLYMSDLVEEL
                     TGYPKEDFIENARRSYVTMIHPEDVPSVARRITEAIGRRLSWEIEYRVLRKDGGERWV
                     YEKGVPVFDEDGALLFLDGFIFDITGRKRVDDQLNSERKRLANVIEGANAGTWECNLQ
                     TGETLLNDKLLELLGHAPGDLDPVTMDVWLSLLHPEDLGQVGDILAGHVREELENCDH
                     EVRMQHRDGHWVWMHCKGRIISRTPDGEPLWMYGTYIDVTDRKQAEKELLEANRRLLA
                     MQKVLKREATRDSLTGVLNRRAILEHLNEELARCRRDGTCLSAAICDIDHFKEVNDTY
                     GHQTGDDVLCGLVRLLRSNLREYDKIGRIGGEEFLLVLPGEAGRSAGFHERLVKSVAE
                     HSIQTRSGAVRITLSLGAITVSGDADTDEILARADKAMYKAKAEGRNRVVFISDAKAP
                     "
BASE COUNT          666 a         1297 c         1011 g          761 t
ORIGIN      
        1 cctccgatgc gttctgcagc ccggcggacg ccgtcggcga ggatatcccc cacgccctcc
       61 cggagcgcga tcatccgcac aagcttcggg agcacggacg cgtttcccca ggaaagatca
      121 agcccctgcg tcatctcagg cgagaggagc cccttctcgg cgcactccat caggaagccg
      181 atggcccctc ccgcggagat cgtgtcgatt cccagctcgt tgcagaggta gccgcagtgg
      241 acggcacagt cgacatcctc gttgagcata tacgcggtga acgagcctat cgtctcgtac
      301 tcgccgccgt cgtgctccgg cgtcttccac tttccgtcct tcacgcgcac ctttctgccg
      361 cacttcatcg ggcacccctt gtagcaccct gttccgccgg tgaagttctc ctcgacaaag
      421 tgcgaggcga tctgcgcacc cttcccccag gagttcgact gccagttctt cgtcgggagg
      481 tcgcccatcg cgtcgcaggc ctcgtagccg cccatcgttc cgagctcggt gaaaccgtag
      541 gtgttggggc tctccttcac gatgcggtgc gcctcgcgga cggccttcat atatccggcg
      601 gcgtctgcgg ggaccacctt tccgctccct ctgaccgaaa cggcgagcac gtttttcgag
      661 ccgaggacgg ccccgcaccc gacccggccc gcagcccgat gcccgaccat gacggaggcg
      721 aacagcacaa ggcgttcgcc gcccgggccg atgacgagcg aatcgtactc cgaatagccg
      781 ccctcgttca gcagcgcatt tttctccagc accgtcctgc ctttcaggtg ctccgcgctt
      841 tcgagctctg cccgaccgtc cgtgatgacg aggtggacgg ggcgttccgc tcttccggag
      901 agaacgacga agtcgtagcc cgctttcttc agcgttcctc ccatgtcgca cccgaccctg
      961 gcctcggccc accctccggt caacggcgac ttgcagcaga aggagaggct tccccctccc
     1021 gggacgaggt tgtccgtcag cggccccgtc gcgaggacaa agagattgtc aggcccgaga
     1081 ggatcggttc cggccttcag ttcatcgtac agaatgcggg cggcgtaccc cgtccctccc
     1141 aggaacgggc gcagcttctc ctccgtcagc agctccgtcg aaacagctcc ggaggagagg
     1201 tcgacgcgca gcacctttcc ggcatatccg gcaagtatat ccattcaatc gtctcccttc
     1261 ttctctcgtt tctccatcct cctaccatac cacacgacgg gcggagagac accccgcccg
     1321 tcgtttttta tacattccgc gtcgaaagaa caggcatccc ttccggcccg cctgtcgggt
     1381 tcctcatgct ttgccccgcc gctccttcgg ccggtggacg tagacgctgc acggcgcctg
     1441 cgccaccacc ttcgcggcca cgctcccgag gaagaagcgc tgcatcggac tcagccccct
     1501 gtggccgatc atgatcatat cggcatcctg ctcctcggca aaacggacga tctgctcata
     1561 gaccttgccc gagcgcactg aaacggacat tctcggaatc ctcattcccg ccgcttccat
     1621 ccggtcttcg atgaacttcg tgaattccgc cgttaccttc tcgtcggacg ggggaagcac
     1681 cacctccccc tcgccgtaga agacaggctc gtgttccatg acggtgatca catgaaggaa
     1741 gagcatgttc gcgtcatgct ctctgttcgc gtagtatacg gcgtagtcca cgaccccttt
     1801 gctggtatcg ctgccgtcga tgggtaccac aacttttttc atccgcaacc cctcctttac
     1861 gcccgtttcg ggcatccgaa ctccagtccc gtcagcgccg gaaggtcttg agtgaagtat
     1921 agcattcccc agtctattta gtcaatttta cgacaacttt tccctctcct gctcaaaaag
     1981 ctctccagcg tcctcccgaa cggccgatac atcttgctcc cggaaagcgc ctgttcaggg
     2041 agccttcgcg tcgctgatga agaccacccg gttgcgcccc tccgccttgg ctttgtacat
     2101 cgccttgtct gcgcgcgcaa gaatttcgtc cgtgtcggcg tcgccggaaa cggtgatcgc
     2161 tccgaggctc agcgtaatgc gcacggctcc cgaacgggtc tggatggagt gttccgcaac
     2221 gcttttcacc agccgttcat gaaaccccgc gctccgcccc gcctctccgg ggagaaccag
     2281 caggaattcc tccccgccta ttcttccgat cttgtcgtac tccctgagat tgctccgcag
     2341 cagccggacc agcccgcaca ggacgtcgtc gcccgtctga tgcccgtacg tatcgttgac
     2401 ctccttgaag tggtcgatat cgcatatcgc cgcgctcagg cacgttccgt cccgccggca
     2461 acgcgccagt tcctcattca gatgctccag tatcgctctc cgattgagca cgcccgtcag
     2521 ggagtcgcgc gtcgcctccc gtttcagcac cttctgcatc gccagaagcc tgcggttggc
     2581 ctccagaagc tccttttccg cctgtttccg gtctgtgacg tcgatatagg tgccgtacat
     2641 ccacaggggt tcgccgtccg gcgttcgcga gatgatccgc cccttgcagt gcatccagac
     2701 ccaatgtccg tccctgtgct gcatccggac ctcgtggtcg cagttctcca gttcttcccg
     2761 cacgtgcccg gcgaggatat ccccgacctg ccccaggtcc tcggggtgca gcaaactcag
     2821 ccacacatcc attgtgacgg ggtcaaggtc gccgggggcg tgccccagaa gctcaagcaa
     2881 cttgtcatta aggagcgtct cgccggtctg caggttgcac tcccaggtcc cggcattcgc
     2941 cccctcgatg acgttggcaa gccgcttcct ctccgaattc agctggtcat ccacacgctt
     3001 ccggccggtg atgtcgaaga taaaaccgtc aagaaagaga agcgctccgt cctcatcgaa
     3061 cacgggaacg cccttctcgt acacccagcg ttcgccgcca tccttgcgga gtacgcggta
     3121 ttcgatctcc caggaaagac gccgtccgat cgcttcagtg atgcgtcgcg ctactgatgg
     3181 gacgtcttcc gggtgaatca tcgtcacata gctgcgcctc gcgttttcaa tgaaatcctc
     3241 cttcgggtag cccgtcagct cttccacaag gtcgctcatg tagagcatcg tccattccgg
     3301 atcggagcgg cagcggaaaa cgacgcccgg tatgttcgcc acaagagacc tgaagcgttc
     3361 ctcgctcata cgcagctcgc atcgttcacg cagcccgcag gcggcgcact ggccgatcgt
     3421 ccgtttgaca ggggggcaca tccggatttc aaagagccgg cgggagttgt ggattgcaac
     3481 gcgaagaagc gactcgggcg tctcttgcgt tacaaaaccc gactgtgcga agacggcgag
     3541 ttcgtctgcg atttcctcgc cggacgaggg aaaaacaagg agcagcggaa ccgggcggac
     3601 ggcgagaagc gaacgcagaa tttccgctgt ttcccgggga gacgcaaaga gcgcgtcggc
     3661 aacgaacagc gcaacacccc cctccgagag cagcctgctg gcttcctcgg cggaggagac
     3721 ttcgacaacg aaaca
//
LOCUS       DAOPKU010000284         3733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27311_length_3733_cov_5.595160, whole genome shotgun sequence.
ACCESSION   DAOPKU010000284 DAOPKU010000000
VERSION     DAOPKU010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27311_length_3733_cov_5.595160"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2945
                     /locus_tag="PK849_09280"
     CDS             <1..2945
                     /locus_tag="PK849_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66357.1"
                     /translation="HPYVDIRGTVSGAVLTFVDLSLVKQSETERRRSDMLRKAVLDSL
                     DASIAVLDREGNMLAINETWRRFARENQAKDFRTLDTGANYFAACACSSDTEDYRDAQ
                     RAMAGIRSVLSGRSESFEMEYPCHSPDEKRWFLMRVTPLLFPEGGAVVAHIDVTKEKV
                     ATQTVKESEQRLLAVLNSIDALVFVTDVDTFEILMINDFGRKIFGDVVGRRCWEAIQK
                     GQTGPCEFCAEAPLLGDDGEPAGVRRWQVFNERTKRWYDCRDSAIRWTDRKYVRLEIA
                     MDITETKKIEEELRVHRDELDRLVAVRTEELSESNRELERQTEFAQEMALKAEAANEA
                     KSAFLANVSHEIRTPINAVIGFTELLLDSTLSSEQRRQAETVRASAETLLFLINEILD
                     FAKIEAGKLELEVVDFDLRNTVEGFAGTLALQAYTKGIELACGVEPSLPSKVRGDPVR
                     IRQILMNLGSNAVKFTQEGETVISVSKESETEETLWIRFSVRDTGIGIPGEKMGLLFT
                     KFTQLDASTTRQYGGTGLGLAISKHLVERMGGSIGVESEHGNGSTFWFVLPFEKNGRG
                     AEAPEAPEGVAGLRILVVDGNAASQAFFLRQFESWGMRAVVSSRTEALDVLRRGFSDE
                     DPFRVVLDADSGRSGEHSLGDAIMNDESLSETVLITLVPLGIRHGINPPDGERRYSLV
                     KPVQSAELAAALTAIALGGTVFSVAHEDGKESSSWLLSCGENARILLVEDNAVNRQVA
                     LALLKKAGVRADTAENGLEALEALRSNDYDLVLMDLQMPVMDGLAATLAIRAGRGDVR
                     NPDVPVVAMTAHALQSDREACMHAGMNGYITKPIRPAELLCELERRLCAETAPARDNR
                     REYVTRTGPRTFNFDDLVERLGDEETAGLIMEAFLKEMPEAMSALEKSLGTGDFLLSG
                     RNAHTIKGVAGNIGGEALREAALQFERAAKDGDTKLLHALRERVHAEYCALKDEIERM
                     LRTLRPPE"
     gene            3205..>3733
                     /locus_tag="PK849_09285"
     CDS             3205..>3733
                     /locus_tag="PK849_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPE66358.1"
                     /translation="MKRGILVVCALAFVFLFALPAAYAAVELKLGHFGPEGHPATIAA
                     AQFAENVEKRTNGEVKIVVYPNNALGSPPEVLEQVIMGVIDMSLSGQDQIAKHVKLFD
                     SVAIPFSMQGYEHADRILDGPFKEWAAPELEKIGLVYINSWEWGFRQITNSKRPILSP
                     DDVKGLKIRTPPAMAY"
BASE COUNT          821 a          991 c         1179 g          742 t
ORIGIN      
        1 ttcacccgta cgtggatatc cgcggaacgg tgagcggcgc cgtgctgacg ttcgtggatc
       61 tctccctggt gaaacagagc gagacggaac ggcggcgttc ggacatgctc cggaaggctg
      121 tgctcgactc cctcgacgcg agcatcgcgg tgctcgacag agaggggaat atgctcgcga
      181 tcaacgaaac gtggcgaaga ttcgcgaggg agaaccaggc gaaggacttc cgcacgctcg
      241 atacgggggc gaactacttc gccgcctgcg cctgttcttc ggatacggag gactacagag
      301 acgcgcaaag agccatggcc ggaatccgat cggtcctctc cggccgttcc gaatcgttcg
      361 agatggagta tccttgccac tcgcccgacg agaaacgctg gttcctgatg cgcgtcacgc
      421 cgcttctctt ccccgaaggg ggggctgtgg tagcgcatat cgacgtgacc aaagagaagg
      481 ttgcgacgca gacagttaaa gagagcgaac agcgccttct ggcagtactc aacagcatcg
      541 acgcgcttgt ctttgtgacg gatgtcgaca cgttcgagat tttgatgatc aacgatttcg
      601 gcaggaaaat cttcggcgac gtcgtcggca ggagatgttg ggaggctatt caaaaaggac
      661 agacggggcc gtgcgagttc tgcgccgaag ctccgcttct cggcgacgac ggagaaccgg
      721 cgggagtacg gcgttggcag gtattcaacg agcgtacgaa gcgctggtac gactgccgcg
      781 attctgcgat acgctggacc gaccgcaaat acgtacgcct tgagattgcg atggatatta
      841 cggaaacgaa aaagatcgaa gaagagcttc gcgtgcatcg cgacgaactg gacaggttgg
      901 ttgcggtgcg gacggaggaa ttgagcgaga gcaaccgtga actggaacgg cagacggagt
      961 ttgcacagga gatggcgctc aaggcggaag cggcaaacga ggcgaaaagc gccttccttg
     1021 caaacgtaag ccacgagatc cgcaccccga tcaacgccgt gatcgggttt accgaactgc
     1081 tgctcgactc cacgctttct tccgagcaac gccgccaggc ggagaccgtc agggcgagcg
     1141 cggaaacgct gctcttcctt atcaacgaaa tcctcgactt tgcaaagatt gaagcgggaa
     1201 agctcgaact ggaagttgtg gatttcgacc tgagaaatac tgtggagggc tttgcgggaa
     1261 cgcttgccct tcaggcctac acgaagggga tcgaactcgc ctgcggcgtc gaaccgtcgc
     1321 ttccgtcgaa ggtgcgcgga gatcccgtgc gcatccggca gattctgatg aatttgggaa
     1381 gcaacgcggt gaagttcacg caggaaggcg agacggtgat ctctgtctcg aaagagtccg
     1441 aaaccgagga aacgctgtgg atccgttttt cggtgcggga tacgggaatc ggtatccccg
     1501 gagagaaaat ggggcttctt ttcaccaagt tcacccagct tgacgcttcg acaactcgcc
     1561 agtacggagg gacaggtctt ggattggcca tatcgaagca tcttgtggag aggatgggcg
     1621 gttccatcgg tgtggagagt gaacacggaa acggttctac gttttggttt gtgcttccct
     1681 tcgaaaaaaa cggccgcggc gcggaagctc cggaagctcc cgagggagtg gcggggctcc
     1741 gtattctcgt cgtcgacggc aacgcggcct cgcaagcctt tttcttgcgc cagttcgagt
     1801 cctggggcat gagagccgtc gtctcctcgc ggacagaggc gcttgacgtt ttacggcgtg
     1861 gtttttcgga cgaagacccc ttccgtgttg tgctcgacgc agattcaggc cgttcaggag
     1921 agcattcgct cggggatgcg atcatgaatg acgagagcct ctcggaaact gttctcatca
     1981 cgcttgtccc tctcggaata cggcacggta tcaatcctcc cgacggagag aggcgctatt
     2041 cattggtgaa gccggtccag agcgccgagc ttgctgcggc gctcaccgcg attgccctcg
     2101 gaggcacggt gttttctgtt gcacacgagg atgggaagga atcttcctca tggcttcttt
     2161 catgcgggga gaacgcgcgt atactgctcg ttgaggacaa tgcagtaaac cggcaggtgg
     2221 cgcttgctct actgaagaag gcgggtgtgc gcgccgatac ggccgaaaac ggtctggaag
     2281 ccttggaagc tctgcgttcg aacgattatg atctggtgct tatggacctt cagatgcctg
     2341 ttatggacgg tctggcggcg accctggcga tacgggccgg gcggggcgat gtgcgcaatc
     2401 cggacgtgcc ggtggttgcg atgacggctc atgcgctcca gagtgacaga gaagcgtgca
     2461 tgcacgcagg aatgaacggc tacatcacca aacccatcag gccggcggag cttctttgcg
     2521 agcttgagcg caggctctgc gcggaaacgg ccccggcgcg agacaatcgg agagaatacg
     2581 ttacgcggac gggaccgcgc acattcaatt tcgacgatct tgtggaacgc cttggcgacg
     2641 aggagacggc cggcctcatt atggaagcct tcctcaagga gatgccggaa gccatgagcg
     2701 ccctggagaa gagtctcggg acgggagatt tcctcctctc ggggaggaat gcccatacga
     2761 tcaaaggcgt ggcgggaaac atcggaggag aggcgcttcg ggaagcggcc cttcagtttg
     2821 agcgagcggc caaagacggt gatacgaagc ttcttcatgc tcttcgggag cgggtgcatg
     2881 cggagtattg cgcgctgaag gatgaaatcg aaagaatgct gcgaacgctt cgccccccgg
     2941 agtgagcccg cggggtaccc ttagcaagat catacggaaa atggagcgtt catggagttt
     3001 ctccggagcg ctccattttt atttttaaag ggagagcaag aaagacttga caaacgcaca
     3061 ctcaatactg aaaatatgaa cgagcatttg atggaatagt gttctgtctg ttgaaacgct
     3121 ctgtgccgtt tctcctctgg ctacgcccgc ctacgggttt ccctggcgga ttcaaggcaa
     3181 caccatcata aaggaggggt ttttgtgaaa cgaggaattc tggtagtgtg cgcgctcgcg
     3241 ttcgtgtttc tgttcgcgct ccctgccgca tacgcggcgg tcgagctgaa actgggtcac
     3301 ttcggaccgg aaggacatcc ggccaccatt gccgccgcgc agtttgcgga gaatgttgaa
     3361 aaacggacga atggcgaagt caagatcgtc gtgtacccga acaatgctct cgggagtccc
     3421 cccgaagtcc tggaacaggt catcatgggc gttatcgaca tgagcctctc aggacaggac
     3481 cagatcgcaa agcacgtgaa gctgttcgac tccgtggcca tccccttctc gatgcagggg
     3541 tacgagcacg cggaccgcat tctcgacggt cccttcaagg agtgggcggc ccccgagctc
     3601 gagaagatcg gcctcgtcta tatcaacagc tgggagtggg gcttccggca gatcaccaac
     3661 tcaaaacgtc ccatcctctc cccagacgat gtgaagggct tgaaaatccg caccccgccc
     3721 gcgatggcgt atc
//
LOCUS       DAOPKU010000285         3730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27345_length_3730_cov_3.444082, whole genome shotgun sequence.
ACCESSION   DAOPKU010000285 DAOPKU010000000
VERSION     DAOPKU010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27345_length_3730_cov_3.444082"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PK849_09290"
     CDS             complement(<1..731)
                     /locus_tag="PK849_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPE66359.1"
                     /translation="MNVYLGLDIGTTNIKMLLVDERGSVVKSMSHPLELSAPRPAWAE
                     QDPEDWWNGVWTLLSSVPSDMNVLRIGLSGQMHTLVPVDSEGNVLRKAILWCDQRTSA
                     ECDEATAAVGGEEKIIEMTGNLIYPGFTLPKILWIRKNEPEVYSRISTCFIAKDYIGW
                     RLTGERAAEWSDASGSSCYDVSARRWRSDLLRTLGIDEAWLPPVMGSSDVRGKLRPEL
                     ASKLGWKQAEVVCGGADNAVAALGLG"
     gene            complement(751..1836)
                     /locus_tag="PK849_09295"
     CDS             complement(751..1836)
                     /locus_tag="PK849_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPE66360.1"
                     /translation="MERTHVAYEKLRTYMAEMLVKGLGFPEETAEISSVALVEADARG
                     HASHGVARIKMYFEEVNDGKDNPKAVPEILHETPISLVIKGNMAPGFASSKLAMERTI
                     AKAKEHGTCMTVVQDSCHFGMAGFWAEKAADAGMMGVALTNTLRAGIPLFGKERLLGT
                     NPICVAIPTGKKPHFMLDMATTTVALGKIEVAARRGGTMPVGWAVDENGADTTDAISV
                     AEINRFDKSPFGGQLYLGGAGETLGGHKGYGLGLLVELLTAGLSLGTPTYDTYINSCG
                     ICHYFQATRLDLFGNEEKIKAHVASILEKLRESPKAAEKERIYIHGEKEFERRKKSME
                     EGVYLDPATWERLDEFADKFGMRRLEP"
     gene            complement(1858..3465)
                     /locus_tag="PK849_09300"
     CDS             complement(1858..3465)
                     /locus_tag="PK849_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPE66361.1"
                     /translation="MNAKRYVGKGILALLALLMLVPATFAADGKLTLLSLNDIHGRIY
                     SEKDAGGLAKAATVVEKLRATDPGNVFFVQVGDIDEGPLFFYFHGKAEMTGLNAMKAD
                     VGTLGNHEFDLGKEAFFEATGYAEFPIVVSNLRFKDGTPAPFPEYVIKTAANGMKVAF
                     FGLVTPALAYTTKGSDDFRAGQDLPAEAERMVNILSAKEKPDVIVLLSHCGIEADRLI
                     AKNTAGIHAIVGGHSHTLMEEGEFVEGPNGWGTLIGQAGSYARHLGVMELALKDGAVS
                     KDSSWRVEALDANVPENETVASLLEPFRKELAERLSTPLANQPEDMDSRKDFIRSSET
                     NLGNFIADAFRWKSGAQVAVMNGGGIRGDQLYPAGTVSYATITNMMPFGNTLCMGKIS
                     GKELLQLMETSGSALIGEKDEYDAYMRTPTGGFLQISGIRVVFDTKKQPLLIDNNAVI
                     RKEGERVVKLEALQQDGSWAPVDPAKEYTIATTDWTSGGGDKYTVLKNNGSFISLEQF
                     ILESTAEYIRFLGEMKAEVDGRITILR"
BASE COUNT          646 a         1112 c         1145 g          827 t
ORIGIN      
        1 ccgagcccga gcgccgcgac cgcgttgtcc gcgccgccgc agacgacctc ggcctgtttc
       61 cagcccagtt tcgaagcaag ttcggggcgg agttttccgc gtacgtcgga agaacccatc
      121 accggaggca gccacgcctc gtcgattccg agcgtgcgca gaaggtcgct ccgccagcgc
      181 cgggcggaga cgtcgtagca cgaacttccg gaggcgtccg accactcggc agcccgctct
      241 ccggtgagcc gccagccgat gtagtccttg gcgataaagc atgtactgat gcggctgtag
      301 acttcgggtt cgttcttgcg gatccagagg atcttcggca gagtgaatcc ggggtagatc
      361 aggttcccgg tcatctcaat gatcttttcc tctcctccga cggcggcggt cgcctcgtcg
      421 cattcggcgg acgttctctg gtcgcaccag aggatcgcct tgcgaagaac gtttccctcg
      481 ctatcgacgg ggacgagcgt atgcatctgc cccgagaggc cgatgcggag gacgttcatg
      541 tcggagggga cggaagagag gagcgtccat acgccgttcc accagtcttc cgggtcctgc
      601 tcggcccagg ccgggcgggg cgcggagagt tcgagtgggt gcgacatgct cttgacgacg
      661 ctcccccgtt cgtccacgag gagcatcttg atgttcgtgg tgccgatgtc caggccaaga
      721 tagacgttca tacggtcccc tccctttggt ttacggttcg agacggcgca taccgaactt
      781 gtcggcgaat tcgtcgagac gctcccatgt tgccgggtcg aggtagacgc cctcttccat
      841 ggatttcttc cggcgctcga actctttctc gccgtggatg tagatacgct ccttttccgc
      901 tgccttgggg ctttcgcgca gcttttccag gatggaggcg acgtgcgcct tgatcttctc
      961 ctcgttgccg aagaggtcga gtcgtgtggc ctggaagtag tggcagattc cgcagctgtt
     1021 gatgtacgtg tcgtacgtcg gagttccgag ggagagccct gcggtgagca gctcgacgag
     1081 caggccgaga ccgtacccct tgtgtcctcc gagggtttct cccgcgccgc cgagatagag
     1141 ctggcctccg aagggactct tgtcgaaacg gttgatctcc gccacgctga tcgcatcagt
     1201 tgtatcggct ccgttttcgt cgacggccca gccgaccggc atcgtgccgc ccctgcgcgc
     1261 cgccacctcg atcttgccga gcgccacggt cgtcgtcgcc atgtcgagca tgaagtgcgg
     1321 cttcttcccc gtggggatgg ccacgcagat aggattcgtc cccaggagac gctccttacc
     1381 gaagaggggg atgcccgccc ggagagtgtt cgtgagcgcg acgcccatca tgcccgcgtc
     1441 ggcggctttc tccgcccaga agcccgccat gccgaagtgg cacgaatcct gcacgacggt
     1501 catgcaggtg ccgtgctcct tcgccttcgc gatcgtgcgt tccatggcga gcttggagga
     1561 ggcgaagccg ggggccatgt tccccttgat caccagggag atcggcgttt cgtggagaat
     1621 ttcggggacg gccttggggt tgtccttgcc gtcgttcacc tcttcgaaat acatcttgat
     1681 gcgcgcaacg ccgtgggatg cgtgcccgcg ggcatcggcc tccacaaggg cgaccgaaga
     1741 tatttcggcc gtttcctcgg gaaagccgag ccctttcaca agcatctccg ccatgtatgt
     1801 gcggagtttt tcgtatgcga cgtgtgtccg ttccattaca gcacctccgt tgatttatta
     1861 ccgcagtata gtgatccgtc cgtcgacttc ggctttcatc tcgcccagga aacggatgta
     1921 ttcggcagtg gattccagga tgaactgctc cagagagatg aaggagccgt tgttcttgag
     1981 gacggtatac ttgtcgccgc cgcccgaggt ccagtcggtc gttgctatcg tgtactcctt
     2041 cgcgggatcg acgggcgccc aggaaccgtc ctgctggagg gcctccagct tcacaacgcg
     2101 ctcgccttcc ttacggatga cggcgttgtt gtcgatcaga aggggctgct tcttcgtgtc
     2161 gaagacgacc cggatacccg agatttgcag gaagccgccg gtcggcgtgc gcatatacgc
     2221 gtcgtactcg tctttttctc cgatcagggc cgatccgctg gtctccatga gttggagaag
     2281 ctccttgcct gagatcttcc ccatgcagag ggtgttgccg aagggcatca tgttcgtgat
     2341 ggtcgcgtag gagactgttc ctgcggggta gagctggtct ccgcggatgc cgccgccgtt
     2401 catcacggcg acttgagcgc cggacttcca gcggaacgcg tcggcgatga agttcccgag
     2461 gttcgtctcg ctgcttcgga tgaagtcctt gcgcgaatcc atgtcttcgg gctggttggc
     2521 gagaggcgtg gaaagacgct ccgccagctc tttgcggaag ggctccagaa gcgatgcgac
     2581 ggtttcgttc tcggggacgt tcgcgtcgag cgcctccacg cgccaggagg agtccttcga
     2641 caccgccccg tccttcagcg cgagctccat cacgccgaga tgcctggcgt acgaacctgc
     2701 ctggccgatc agcgtacccc atccgttcgg tccttcaaca aattccccct cttccatgag
     2761 ggtatgactg tgccctccga cgatcgcatg aataccggcg gtgttcttcg cgatcaaccg
     2821 gtcggcttcg atgccgcagt gactcaacag cacgataaca tcgggttttt ctttcgccga
     2881 aagaatgttc accatccgtt cggcctccgc gggaaggtcc tgaccggcgc ggaagtcgtc
     2941 gctccccttt gtggtgtacg ccagcgcagg ggtgaccagc ccgaagaagg cgactttcat
     3001 cccgtttgcg gcggtcttga tcacgtactc ggggaaggga gccggggttc cgtccttgaa
     3061 acgaaggttg gagacgacga tcgggaactc cgcgtaccct gtggcctcga aaaaggcttc
     3121 tttcccaaga tcgaactcat ggtttccgag agtaccgaca tccgctttca tcgcgttcag
     3181 gccggtcatc tcggccttcc cgtgaaagta gaaaaagagc ggtccttcgt cgatatcgcc
     3241 gacctgaaca aaaaagacgt tgcccggatc ggtcgcacga agcttttcta cgactgtcgc
     3301 cgcttttgcg agcccgcctg catccttttc ggagtagatc cgtccgtgga tgtcgttgag
     3361 ggagagcagc gtcagttttc cgtcggcggc gaaagttgca ggaacgagca taaggagagc
     3421 gagcagtgct aaaattcctt ttcctacgta tcgtttcgcg ttcatagcaa aaacctcctc
     3481 gtggaaaaat tgtccgcttc gtgtttgaga cgagcggata cggggtgatt atagcaccaa
     3541 cacgcttggt aggacgagaa aagacgggct gagtttcctc ggcccgtctt tccgtgaacg
     3601 atggtttcag gagagttccg gggtgctctt cggcggcctg cggaagtgtg tgatctgttc
     3661 aaatgcgtag gcgatcgaga tgagcgttcc ctcgctccag ggacgcccca gaatttcaag
     3721 accgataggg
//
LOCUS       DAOPKU010000286         3720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27459_length_3720_cov_3.661664, whole genome shotgun sequence.
ACCESSION   DAOPKU010000286 DAOPKU010000000
VERSION     DAOPKU010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27459_length_3720_cov_3.661664"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..344
                     /locus_tag="PK849_09305"
                     /pseudo
     CDS             99..344
                     /locus_tag="PK849_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013199.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(473..1591)
                     /locus_tag="PK849_09310"
     CDS             complement(473..1591)
                     /locus_tag="PK849_09310"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPE66362.1"
                     /translation="MKLYWKILLLALLPLLLALGLGFFRLELIVRFQRSGREAADELK
                     MALLNSRFKAYREDRSRNAAVLAGANDAVRAVQNSDTGFLVPWGELFLDPLRYSLIFF
                     TDGDGIVLARAHAPYRFGDDISAHPAVAAALKGRRSVGLFELDGEPALLETRPVMLYG
                     EIQVGTVGIGAPLTKELLAHFSEGTGLGIEVEIEGFPPISMEECYGNTERKSVALFPS
                     VSEESLKFRRIEAVFTEDELGADLRYFQRNLVLTMLLLSVTLLSGLFLLLRRYLRPFS
                     LLVEDVTLLSGNQEDFFELRKRLAHNYLDTSHEVSVIAHSLSRLMERIQETITLLEWT
                     SRTDPLTRISNRLHLDSVLEQEMKASAKTGSPLSRSKT"
     gene            complement(1588..2511)
                     /locus_tag="PK849_09315"
     CDS             complement(1588..2511)
                     /locus_tag="PK849_09315"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HPE66363.1"
                     /translation="MKTQRVRPVSRSCFRAFLLLLVALCFLRLSSADASENYIFAVYP
                     VNDPDRMLAAFRPLADFIAAETGVPVRLVVTGNYEEMTRRLADGSVHFAFFTSSGYVK
                     HASSIPGLVYLATYAELDAKGLVTPYYHSVILSLEEGPVDALADLKGRNFGFTDKDST
                     SGYKVPLTMLRALGIDPSSFFGKLFFLGRHDAVVEALRAGSIQGGAVSDGTLHNAVRQ
                     YGDVFRVLAVSESIPLDAVVAASGVAAEHAEAVRRGLASLAPDSAPMSAFREQFGWPA
                     AGFVVLGDEFYEPLRRALEPAEDAGEQEADG"
     gene            2829..>3720
                     /locus_tag="PK849_09320"
     CDS             2829..>3720
                     /locus_tag="PK849_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66364.1"
                     /translation="MKKSVWTIVVPAITLLFIAGFLYVRRPFTVPVTLFYDERDLAGA
                     VVSKQALQSVVLAMEFFNSRSSEFRFLPFRETDQDIYESIEHAAARGSVAIVGGINAP
                     FASLLADSSRRHGIPFLSLASGTSLARSDDFVFRARPRNGGKELGLAAKSMGVSSYSV
                     IVSGFAASHVQEFIRDFESQLGAPPRRTMVFSGNLNKHIEDFERIAKGVDAMLLVLPD
                     WLAAVALRELRLRLPGLPVFASNWAISHRTHLLAGSLGEGVLTVSFTPPEWDSQDHEF
                     LRFVQDTYASPIPPVILAMGY"
BASE COUNT          721 a         1160 c         1142 g          697 t
ORIGIN      
        1 caatcttcga agacctgctg atcgtcggaa cgaaggtcgg cgagaccgac ggcgccgcgc
       61 ccggtgacat ccgcgccttc gatctgcaga ccggcgcgat gcgctggatc ttccacacca
      121 tcccccgcga aggcgagttc ggcgccgaga cctggccgaa agacgcctgg cagacccgcg
      181 gcggcgcgaa ctcctggtcc ggcgtcaccc tcgatcgaga gcgcggcctc gtcttcctgc
      241 cgacgggctc ggcgacgccg gatttctggg gcggcgatcg cgcgggcgac aatctcttcg
      301 cgaacagcct gctcgccctc gatgcgcgca cgggcgagcg ccgctggcat ttccagttcg
      361 tccaccacga cctctgggat cgcgacctgc ccgcgccgcc gacgctcgtg acgatcgagc
      421 gggacgggcg cacgatcgac gccgtcgccc agaccacgaa ggcaggctac gtctatgtct
      481 tcgagcgcga gagcggcgag cccgtcttcg cggatgcctt catctcctgt tcaagcacgc
      541 tgtcgaggtg gagccggttg ctgatcctgg tgagcgggtc ggtgcgggag gtccattcga
      601 gcagcgtgat cgtctcctgg atgcgctcca tcagtctgga gagggagtgc gcgatcacgg
      661 agacctcgtg gctcgtatca aggtagttgt gcgcgagcct tttgcgcagt tcgaagaagt
      721 cctcctgatt tccggagagc agcgtcacgt cctccacgag cagcgaaaag gggcggaggt
      781 agcggcgaag cagcaggaag aggccggaga gcagcgttac ggagaggagg agcatcgtca
      841 gcacgagatt tctttgaaaa taccgcaggt ccgcgccgag ctcgtcctct gtgaacaccg
      901 cttcgatccg ccggaacttc aggctctcct ccgaaacgga aggaaacagc gcgacggact
      961 tcctttcggt atttccgtag cattcctcca tggaaatggg cgggaacccc tctatctcga
     1021 cttctatccc cagtccggta ccctcggaaa agtgcgcgag gagctccttc gtcagagggg
     1081 cgccgatgcc gaccgttccc acctgaatct ctccgtagag catgaccgga cgcgtttcga
     1141 gcagcgccgg ttcgccgtcc agttcgaaca gtccgacgga gcgccgccct ttcagagccg
     1201 ccgcaaccgc tggatgcgcg ctgatatcgt cgccgaaacg gtagggggcg tgcgccctgg
     1261 cgagaacgat tccgtccccg tcggtaaaaa agatcaggga gtagcggagg gggtcgagga
     1321 aaagttctcc ccacgggaca agaaaaccgg tgtcgctgtt ctggacggcc cgcacggcgt
     1381 cgttcgcccc ggccaacacc gcggcgttcc tcgaccggtc ctcgcggtag gctttgaacc
     1441 tcgagttcag gagcgccatc ttcagttcat ccgcggcctc ccgtcccgaa cgctgaaaac
     1501 ggacgatcag ttcgagcctg aaaaaaccga gccccagggc gagcaacagg gggagaagcg
     1561 cgagaagcag tattttccag tacagcttca tccatccgcc tcctgctctc cggcgtcttc
     1621 ggctggttca agcgccctcc gcaacggttc gtagaactcg tcgccgagga cgacgaagcc
     1681 tgcggcgggc cagccgaact gctccctgaa ggcgctcatg ggggcggaat cgggcgcgag
     1741 cgaggccagt cccctgcgca ccgcctcagc atgctccgcg gcgactccgg aagcggcgac
     1801 cacggcgtcc aggggaatgg attcggagac ggcaagcacg cgaaagacat cgccgtactg
     1861 cctgacggcg ttgtgcagcg ttccgtccga caccgcccct ccctggatgg agcccgccct
     1921 gagcgcctcg acgaccgcat cgtgcctccc gaggaagaag agcttgccga agaacgacga
     1981 ggggtcgatg ccaagcgcac ggagcatcgt cagaggaacc ttgtaccccg aggtggagtc
     2041 cttgtcggtg aagccgaagt tgcgcccctt cagatcggcg agggcgtcga caggcccctc
     2101 ttcgagagag agaatcaccg aatgatagta cggcgtaacg agtccctttg catcgagctc
     2161 cgcgtaggtg gccaggtaga cgagcccggg tatggaggag gcgtgcttca cgtagccgga
     2221 ggaggtgaag aaggcgaagt ggacggagcc gtcggcgagc cgcctggtca tctcctcgta
     2281 gtttccggtg acgacgagcc ggacgggaac gcccgtctcc gcggcgatga agtcggcgag
     2341 cggccggaag gcggcaagca ttctgtccgg gtcgttgact gggtagacgg cgaaaatata
     2401 gttttccgat gcgtccgccg aggaaagccg caggaagcag agagcaacga gaagcaacag
     2461 gaaagctctg aaacaactcc gtgaaacagg gcgtacgcgc tgcgtcttca tacttccacc
     2521 tccgtcgtct ctcgaatgcg tacaaacggg gaaagattct caacaagaaa atagcacctc
     2581 tttctcctgc ggtcaagcac attccgtcgt ctcgcagttt cgggaaggct ctccggaagg
     2641 cgttttcgac cggagaggcg cgcaaaaaaa gtcccgcccc gatatttgtc aacagaaaat
     2701 gattttcaca agacgaaaat attggtataa tcgctgtgtg ttcgatgtat ttttgttaca
     2761 agaagggcaa gtagactctt ggagccttca gacctacaat aaaacatgcc gccttggagg
     2821 gctattttgt gaaaaaaagt gtttggacga tcgtcgtacc ggcgatcaca ctccttttta
     2881 tcgccggttt tctgtatgta cgcaggccgt tcacggttcc ggtgacgctt ttctacgacg
     2941 aacgagatct tgccggggct gtagtgtcca aacaggctct tcagtccgtg gtgctcgcca
     3001 tggaattctt caactcccgt tcctcggagt ttcgtttcct tcctttcaga gagaccgatc
     3061 aggatattta tgaatccatc gaacatgccg ccgcaagagg ctccgtagcg atcgtcggag
     3121 gaatcaacgc gccgttcgcc tccctccttg cggattcctc caggcgtcac ggcattccat
     3181 ttctctccct ggcctcgggc acgtcgctcg cgcgttcgga cgacttcgtc ttcagagcgc
     3241 gtccccgtaa cgggggcaag gagctcggac tcgctgcaaa gagcatgggg gtctcctcct
     3301 actcggtgat cgtcagcgga ttcgccgcct cgcacgtgca ggagttcatc cgcgacttcg
     3361 agtcgcagct cggagctccc ccgcgcagga cgatggtctt cagcggcaac ctgaacaaac
     3421 acatcgagga cttcgagcgc atcgccaagg gagtggacgc catgcttctc gtccttcccg
     3481 actggcttgc ggccgtcgcg ctgcgggagc ttcggctccg gctgcccggc ctccccgtct
     3541 tcgcctccaa ctgggctatc tcgcacagga cgcatctgct cgccggctcg ctcggagagg
     3601 gcgttctcac ggtttcgttc acccccccgg agtgggacag ccaggaccac gagtttctcc
     3661 gcttcgtgca ggacacgtac gcgtccccca ttcctccggt catcctcgcg atggggtacg
//
LOCUS       DAOPKU010000287         3720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27461_length_3720_cov_3.475580, whole genome shotgun sequence.
ACCESSION   DAOPKU010000287 DAOPKU010000000
VERSION     DAOPKU010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27461_length_3720_cov_3.475580"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PK849_09325"
     CDS             <1..839
                     /locus_tag="PK849_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66365.1"
                     /translation="FMRFSSGAALARVVAEKNNPQVDMILGGPADTYEAGIKEGVFEQ
                     YKPAEADMIPERFRSPENYWTGVGIIPLVFLTNGKFLKEKGLEAPASWNALLDPAYKN
                     GLQMADARTSGTATERIYALVKIMGEDEAFAYQKKLHGNIQMYTKSGAGGAMPIATGQ
                     AASGIFYIVDALEIQQQGHDVVLSYPVEGVSFGIEATGILKGAKNLENAKKFMDWASS
                     KTFAQLLVDRKINYIPTRTDVETTNPALDVSKVKLVEVDVAWKGANRKAFVDRWINEV
                     IK"
     gene            934..2631
                     /locus_tag="PK849_09330"
     CDS             934..2631
                     /locus_tag="PK849_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPE66366.1"
                     /translation="MTQPTAPHATSKVSLLKRDPALLPALIAIWISLAVFVLYPLFRL
                     FLTTFVVEGQISFTNLAVVLESWYDRQAFVNSIWLATAVSVAGTFLGYVYAFAVTRIP
                     MPLWLKWFLGAVTTLPLISPPFTSSIALTLSLGPNGILLKLLGIGNFNFYGFWGTWMS
                     ETLTYFPVSFLTLAAVLTAIDPNLEDAGLSLGASPGRVFRTVTLPLSVPGIANSFLLL
                     FASSLADFATPLVLGGHSFPVLPTQAYLQITGMYDLRGGAALSFLLLLPAMAVYFLQR
                     FWVGRKNYVTISGKTGSRSGFKSKGKFAEGLVLAVCFSVTAFILYLYSLILWGAIVKV
                     WGVDNTLTMENFRYVFTHGKKAIIDTLTIAAVATPLGGLMAVVIGYIAQRKRFFGNRA
                     MEFFSMLNYALPGTVVGIAYVIAFNERPILLTGTMTILVAAYMFRYHAAGIRAVIASL
                     HQIDPSIEEASSSLGAGAVRTFFRVTVPLMIPAVLMGMRYLFIHSMTAISATIFLVSV
                     RWSLLTTRILECMTELQFAQACAFSVVLILLVFAATAVLMGLTRFATRRISGEGGRN"
     gene            2631..3674
                     /locus_tag="PK849_09335"
     CDS             2631..3674
                     /locus_tag="PK849_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66367.1"
                     /translation="MAVTLELSNVSKVFQKDREQVRAVDSVSLSVAPGEMVTFLGPSG
                     CGKTTTLRMVAGFEIPSEGQITIDGRDVTNVPVNRRDIGFVFQNYALFPHMSVFENVA
                     YGLRVKGDRETEIRRKVREGLDLVGLGKAEHRFPNQLSGGEQQRVALARVLVLQPRVL
                     LMDEPLSNLDAKLRIHMRTEIRRIQKHLNVTCLYVTHDQGEALTMSDRIMVMHRGKVE
                     QVGAPMEIYSDPRSLFVADFIGQANILPGEVRAVEGGTLSVDVAGHVFPVRAVKGGSF
                     TTGDAAAVVVRPENLLPSDGGGIPGTVETATFLGGRMEYEVLLASGRTALSFDPFLPG
                     KRLWSEGESVSLV"
BASE COUNT          641 a         1229 c         1127 g          723 t
ORIGIN      
        1 acttcatgcg gttctcgtcc ggagcggcgc tggccagggt agtggcggag aagaacaatc
       61 cgcaggtcga catgatcctc ggaggccccg ccgacaccta cgaagccggc atcaaggagg
      121 gcgtcttcga acagtacaaa cccgccgagg ccgacatgat cccggagcgc ttccgctctc
      181 ccgagaacta ctggaccggc gtgggcatca tccctctggt cttcctgacc aacggaaagt
      241 tcctcaagga gaaggggctt gaagcacccg cctcctggaa cgctcttctt gacccggcgt
      301 acaagaacgg cctccagatg gccgacgccc gcacctccgg cacggcgacc gagcgcatct
      361 acgccctcgt gaagatcatg ggcgaggacg aggcgttcgc ctatcagaag aagctccacg
      421 ggaacatcca gatgtacacc aagagcggcg cgggcggcgc gatgcccatc gcgaccggac
      481 aggccgcctc cggaatcttc tacatcgtgg acgcgctgga gatccagcag caggggcacg
      541 acgtggtgct gagctacccc gtcgagggag tctccttcgg catcgaggcc acaggcattc
      601 tgaagggcgc gaaaaacctt gaaaacgcga agaagttcat ggactgggcg tcgtcgaaga
      661 cgttcgcgca gctgctcgtc gacaggaaga tcaactacat cccgacgcgc accgacgtgg
      721 agaccacgaa ccccgcgctc gacgtttcta aggtcaagct ggtcgaggtc gacgttgcgt
      781 ggaagggcgc caaccggaag gcctttgtcg accgctggat caacgaggtt atcaagtagc
      841 ggactcattc cggtttttgg aaaacctgcc cccggcggac gctctttccg ccgggttttt
      901 tacctttgat gttcaggcaa ggagattgaa cttatgacac aaccaacggc gccgcacgca
      961 acgagtaaag tatctctcct gaagcgggat cccgcgctgc tccccgcgct catcgccatc
     1021 tggatatcgc tcgcggtctt cgttctctac ccgctcttcc ggctttttct gaccacgttc
     1081 gtcgtggaag ggcagatctc gttcacgaac ctcgcagttg tgctcgaaag ctggtatgac
     1141 agacaggcct tcgtcaacag catctggctg gccacggccg tctccgtcgc ggggaccttc
     1201 ctcggatacg tctacgcctt cgcggtgacg cggattccga tgcctctctg gctgaagtgg
     1261 ttcctggggg ccgtcaccac gctccccctg atctcgcccc ccttcacgag cagcatcgcg
     1321 cttaccctct cgctggggcc caacggcata ctgctgaagc tgctgggcat cgggaacttc
     1381 aacttctacg gcttctgggg gacgtggatg tcggagactt taacctattt ccccgtttct
     1441 tttctcaccc tggcggcggt gctcaccgca atcgacccga acctcgagga cgccgggctc
     1501 tcgctgggcg cttcgcccgg gcgcgtcttc cgcactgtga cgctcccgct ctccgttccc
     1561 ggcatcgcga actccttcct gctgctcttc gccagctcgc tggccgactt tgccacgccg
     1621 ctggtgttgg gcgggcacag cttccccgtt ctcccgacgc aggcatacct tcagatcacg
     1681 ggcatgtacg atctccgggg cggggcggcg ctctccttcc tgctgctgct gcccgccatg
     1741 gcggtctact ttctgcagcg cttctgggtg gggcggaaga actacgtcac catctcgggc
     1801 aagacgggga gccgctccgg cttcaagagc aaggggaagt tcgccgaagg gctcgtgctg
     1861 gccgtctgtt tctccgtgac ggcgttcatc ctctacctgt actcgctcat actctggggg
     1921 gcgatcgtga aggtctgggg cgtcgacaac acgctgacca tggagaactt ccgctacgtc
     1981 ttcactcacg ggaagaaggc gatcatcgac acgctgacca tcgcggcggt ggcgactccg
     2041 ctcggcggcc tcatggccgt cgttatcggg tacatcgcgc agcgcaaacg attcttcggc
     2101 aaccgcgcca tggagttctt ctccatgctg aactacgcgc tccccgggac ggtcgtcggc
     2161 atcgcgtacg tcatcgcgtt caacgagcgg ccgattctgc tcaccgggac gatgacgatc
     2221 cttgtcgccg cgtacatgtt ccgctatcac gccgcgggca ttcgcgccgt gatcgcctcg
     2281 ctccaccaga tcgatccctc catcgaggag gcttcctcaa gcctgggagc gggcgcggtg
     2341 cgcaccttct tccgcgtgac ggtgccgctg atgatccccg ctgttcttat gggaatgcgc
     2401 taccttttca tccactcgat gacggcgatc agcgcgacga tcttcctcgt ctccgtccgt
     2461 tggagcctgc tgaccacgcg catcctggag tgcatgaccg agctccagtt cgcccaggcg
     2521 tgcgccttct ccgtggtgct catcctgctt gtttttgccg ccaccgccgt actgatgggg
     2581 ctgacccgct tcgcaacgag gcgcatttcc ggtgaaggag gccgcaactg atggccgtaa
     2641 cgcttgaact ttccaacgtc tccaaagtct tccagaagga ccgggagcag gtccgcgccg
     2701 tggactcggt ctccctttcg gtcgcgccgg gcgagatggt gacctttctt ggcccctccg
     2761 gctgcggcaa gacgacgacc ctccgcatgg tggcaggctt cgagatcccc tcggaggggc
     2821 agatcaccat cgacgggcgc gacgtcacca acgtccctgt gaaccgacgg gacatcggtt
     2881 tcgtcttcca gaactacgcg ctcttcccgc acatgagcgt cttcgagaac gtggcgtacg
     2941 gtctgcgcgt gaagggcgac cgggagacgg agatccgcag gaaggtccgc gaagggctcg
     3001 accttgtggg gctgggcaag gcggagcacc gctttccgaa ccagctctcg ggaggggagc
     3061 agcagcgcgt cgcccttgcg cgagtgctcg tgctccagcc ccgcgtgctg ctgatggacg
     3121 agccgctgtc caacctcgac gcgaagctgc gcatccacat gcgcaccgag atccgccgaa
     3181 tccagaagca cctgaacgtc acctgcctct acgtcacgca cgaccagggg gaggcgctca
     3241 ccatgtccga ccggatcatg gtgatgcaca gggggaaggt ggagcaggtc ggcgcgccga
     3301 tggagattta cagcgaccct cgctccctct tcgtggcgga cttcatcggc caagcgaaca
     3361 tccttcccgg cgaggtgcgt gctgtggagg gcggaacgct ctccgtcgac gtcgccggac
     3421 acgtcttccc ggtgcgcgcg gtcaagggag gctcgtttac gacgggcgac gcggccgcgg
     3481 tggtcgtccg cccggagaac cttctcccgt ccgacggcgg cggcatcccc ggaactgtgg
     3541 aaaccgccac tttcctcggc gggcggatgg agtacgaagt cctcctcgca agcggccgga
     3601 ccgcgctctc cttcgacccc ttcctgcccg ggaagcggct atggagcgag ggggagtccg
     3661 tgagcctcgt ctagacttac ttcttcgaat ctaatcggcc tgtgataggc cagaacgctc
//
LOCUS       DAOPKU010000288         3713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27523_length_3713_cov_5.405960, whole genome shotgun sequence.
ACCESSION   DAOPKU010000288 DAOPKU010000000
VERSION     DAOPKU010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27523_length_3713_cov_5.405960"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PK849_09340"
     CDS             complement(<1..739)
                     /locus_tag="PK849_09340"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPE66368.1"
                     /translation="MTSSISKCLFPVAGLGTRFLPATKEIAKEMLPLVDRPIIEYGVE
                     EALAAGCKEMVMITGRAKKAIEDYFDRSFELEELLKSRNKQTLYDEVISLSEMADFIY
                     LRQHRPLGLGHAVLCGEPVCRNEYFGVILPDDVMVAEPSVLAQLLAVHSERGGSVVAL
                     EEVSAEDTSRYGVVAAENAGNGVFAVKDMVEKPGPEKAPSRLAIMGRYILSPAIFPIL
                     ERQQAGAGGEIQLTDGIRTLISQEPVWG"
     gene            complement(765..2135)
                     /gene="glmM"
                     /locus_tag="PK849_09345"
     CDS             complement(765..2135)
                     /gene="glmM"
                     /locus_tag="PK849_09345"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPE66369.1"
                     /translation="METGEKKVRCLFGTDGVRDVANRGVMTPEMALRLGRSYILFLTE
                     RGIPRPRIVVGRDTRRSGHMLEAALSAGMASAGAEVFLLGVLPTPGVSFAVKKMSVHG
                     GAVISASHNPAEYNGIKFLDSSGKKLSDDVEGAIEEYMGDNLTDDWRPTGASVGDIHR
                     ATHLLDEYSEWIASLWGEETPFPSAGVIDCAHGAAVETAEKVFRRLGGEWRFIGNRPD
                     GLNINEGVGVMHMAHIAEAVRTNALPIGIAYDGDADRALLTDCRGRVLNGDIILWVLA
                     RWLAERNMLGSGVVATVMSNMALEDHLRKENIPVFRCPVGDRYVMETMSKEGSLLGGE
                     QSGHIIISSFVNTGDGLCTGLFFLKACLELGEDISTLADRFAPYPQILRNVEVSRDGK
                     VSGDFLRDLSSEAETLLQGAGRILIRPSGTEPLMRILVEARDEKLMEHVSEKLVNAIK
                     EQCRYQ"
     gene            complement(2167..3393)
                     /locus_tag="PK849_09350"
     CDS             complement(2167..3393)
                     /locus_tag="PK849_09350"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPE66370.1"
                     /translation="MSESGKIDAILASPLFLKIMAGVIALALWFYVSGNRSTETTRTL
                     VVPLEYLNVSPQTTLKTAVQEVEIVLSGAQYVLSAIPQDGVVCEVDLRGVGVGKYRIP
                     VRSIVPKDVKIMDVKPTHVDVELARFIDRLIPVEIAVDKGLPPGLFLDMVEILPKNVS
                     VKGSERDIAKIDAVKIVPTLDELKAGGEHTLAVEVVKSEAFEDEVVVEPKTVKLSAVL
                     AEGVPKKSVPVNARIIGEPGGDFKLKAVVVEPAVVTVEGPLAKLNAVTRVDTETIDLT
                     GISKEQNIVIPLRAPEDQMVTIVGAASVRINVLLTPFTVTRLLSRLNVEVDGRSVYPG
                     WKVEPNIVNVTVEGVPSEVNALDEKDVVIRPFVNVTNVVSRRLTVPVQIQNKSGGKLK
                     VVKIDPASVTVIADIR"
     gene            complement(3419..>3713)
                     /locus_tag="PK849_09355"
     CDS             complement(3419..>3713)
                     /locus_tag="PK849_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HPE66371.1"
                     /translation="RIIAASCYLPLTENSDLSRWIGTRHRAALGVTEVSDAIALVVSE
                     ERGEVSLAINGRLSKNLKEAQVHKLLSHYFSGEEAEHKPLMERLQEDLRALWS"
BASE COUNT          750 a         1128 c         1027 g          808 t
ORIGIN      
        1 cgccccagac aggctcttgg ctgatcagcg ttcgaatgcc gtcggtcagc tgaatctctc
       61 ctcccgctcc cgcctgctgg cgctcaagaa tcgggaagat tgccggagag agtatgtagc
      121 gtcccatgat agcaagcctg ctcggggctt tttccggtcc cggcttctcc accatgtcct
      181 ttactgcgaa aacgccgttc ccggcgttct cggcggcaac gacgccgtaa cgcgacgtgt
      241 cttccgcaga gacttcttca agagcgacga cgctgcctcc tcgctcgctg tgcactgcaa
      301 gaagctgcgc aaggaccgag ggttcggcaa ccataacgtc gtcgggaagg atgacgccga
      361 aatactcgtt gcggcagacc ggttccccgc agagcactgc atgccccagg ccgaggggac
      421 gatgctgccg gaggtagatg aaatccgcca tttcggagag cgagattacc tcgtcgtaga
      481 gggtctgctt gttccgtgac ttaagcagct cttcaagttc aaaagagcgg tcgaagtagt
      541 cttcgattgc cttctttgcc cggccggtga tcatgaccat ctctttgcat cccgcggcaa
      601 gcgcttcttc aacgccgtat tcaatgatgg gtctgtcgac gagcggcagc atttctttcg
      661 cgatctcttt tgtcgcgggg aggaaacgcg ttcccagtcc cgccacgggg aagaggcatt
      721 ttgatatcga agaagtcata cggcaaccac ctttcacacg atcgttattg atagcggcac
      781 tgctctttga tggcattcac gagcttctcg gaaacgtgct ccataagctt ttcatcccgc
      841 gcttcaacaa ggattcgcat cagaggctcc gtgcccgagg ggcggatgag aattcttccg
      901 gcgccctgta gcagcgtctc cgcttcggag gagaggtccc tcaggaagtc gccgctcacc
      961 ttcccgtcgc gggaaacttc gacgttgcgc agaatctgcg gatacggggc gaaacggtct
     1021 gcgagggtcg aaatatcttc accgagctcc agacacgcct tgaggaagaa aaggccggta
     1081 cagagtccgt cgccggtgtt cacaaacgag gagataatga tgtggccgga ctgttctcct
     1141 ccgagcagcg atccctcctt ggacatcgtc tccataacgt atcggtcgcc cacagggcag
     1201 cgaaaaacag ggatattttc tttccggaga tgatcttcga gcgccatgtt gctcattacc
     1261 gtggcaacga ctcccgagcc gagcatattg cgctcggcga gccagcgcgc gagtacccag
     1321 aggatgatat cgccgttgag tactctccct ctgcaatccg tcagcagcgc gcggtcggcg
     1381 tctccgtcgt aggcgatgcc tatcgggagc gcgttcgttc tcaccgcttc agctatgtgc
     1441 gccatgtgca taacgccgac gccttcgttg atattcagtc cgtcgggccg gtttccaata
     1501 aagcgccact cgccgccgag cctgcggaag actttttcag ccgtttccac cgccgcgccg
     1561 tgcgcgcagt cgataacccc cgccgacggg aagggggtct cttcccccca gagcgaggca
     1621 atccactcgg aatattcatc aagcagatgc gtggctctat gaatatctcc cacggaagcg
     1681 ccggtggggc gccaatcgtc ggtgaggtta tcccccatgt actcttcgat cgccccctcg
     1741 acgtcgtcgg aaagtttttt cccggatgaa tcgaggaatt ttattccgtt gtactccgca
     1801 gggttgtggg aggcgctgat caccgctccg ccgtgaaccg acatcttctt tacggcgaaa
     1861 ctgacgcccg gagtaggcaa cactccgagg agaaagacct ccgcgccggc cgaagccatg
     1921 ccggcggaaa gagcggcttc cagcatgtgc ccggaacgcc tggtgtccct tccgacgacg
     1981 atccttggac gagggatgcc cctctccgtc aggaaaagaa tgtaggaacg accgagccgc
     2041 aatgccatct cgggagtcat cactccccta ttggccacgt cccgcacacc atcggtcccg
     2101 aaaagacaac gtactttttt ctcaccagtt tccatgcagc ctcaccatcc ccgcccgtat
     2161 aagagattac ctgatatccg caattacagt gacgctcgca ggatcaattt ttactacctt
     2221 caatttaccg cccgacttgt tctgaatctg cacaggaacg gtcaagcgtc tggaaacgac
     2281 gttggtgacg ttgacaaagg ggcgtatcac cacgtctttt tcgtcgagtg cgttcacttc
     2341 ggaggggact ccctccacag tgacgttcac aatgttcggc tccactttcc acccggggta
     2401 tacgcttcgc ccatcgacct cgacgttcag ccgggagagg aggcgggtga cggtgaaggg
     2461 ggtcagaaga acgttgatcc gcaccgaagc ggcgccgaca atggtcacca tctgatcctc
     2521 cggagcgcgc aacgggatga cgatgttctg ctccttggag atccccgtca ggtcgatcgt
     2581 ttcggtatca acccgcgtca cggcgttgag tttcgccaga ggcccctcga ctgtgacaac
     2641 ggcaggttca acgacaacgg ccttcagttt gaagtcgccg cccggttcgc cgataatccg
     2701 ggcgttcacc gggacgctct tcttcggaac tccctcggcg agcaccgcgc tgagcttgac
     2761 ggttttcggc tccacaacaa cctcatcttc aaacgcctcc gacttgacga cctcgacggc
     2821 aagcgtatgc tcccctcccg ccttcagctc atcgagggtg ggaactatct tgacggcgtc
     2881 tatcttcgcg atatcgcgtt cgctcccctt gacgctgacg tttttgggaa gtatctcgac
     2941 catatcgaga aaaagccccg gaggaaggcc tttgtcgacg gcgatttcca ccggaatgag
     3001 gcggtcgata aagcgagcga gttccacgtc gacgtgcgta ggtttgacgt ccattatctt
     3061 gacgtccttg ggaacgatgg aacggacagg tatccggtat tttccgacgc ccactcccct
     3121 gaggtcgacc tcgcagacca cgccgtcctg aggaatggcg gagagaacat actgcgcgcc
     3181 cgagaggact atctccactt cctgcacagc ggttttgagc gttgtctgcg gcgagacgtt
     3241 gagatactcg agaggaacaa ccaatgtcct cgtcgtctcg gtcgatctgt tgccggagac
     3301 gtaaaaccag agagcgagcg cgatcacccc ggccattatt ttcaggaaca gcggcgacgc
     3361 caggatcgcg tctatctttc cggattccga cataagcgcc tgcgcacctt cttcccgttc
     3421 atgaccacag cgcccgcaga tcttcctgca ggcgttccat gagtggttta tgttcagctt
     3481 cttcaccgct gaaatagtgg gagagcagtt tatgcacctg ggcctccttc aggtttttgg
     3541 aaaggcggcc gttgattgca agcgaaactt ctccccgttc ttcggagacg acaagggcga
     3601 tcgcgtcgga aacttccgtc acgccgagcg ccgccctgtg acgggtgccg atccatcgcg
     3661 acagatcgga gttttccgtg agcggcaaat agcagcttgc agcgatgatc ctc
//
LOCUS       DAOPKU010000289         3697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27707_length_3697_cov_3.485997, whole genome shotgun sequence.
ACCESSION   DAOPKU010000289 DAOPKU010000000
VERSION     DAOPKU010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27707_length_3697_cov_3.485997"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PK849_09360"
     CDS             <1..556
                     /locus_tag="PK849_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE66372.1"
                     /translation="LESFTTGGLSLSDGISYGFGPSISWPIFHGGAIRSNIKIQTAKQ
                     EQLLAAYEQTVLNAVAEVRNALTSETQERERNQSLRRGVDAARTAFEIAEDKYKNGLT
                     DFNNVIGAQSALLTLEEQYAISEGQMLSNIVRIFKVLGGGWAPLAAGGFPQPVDGDTA
                     KTTESQISSESRSYLEQIRQELKD"
     gene            complement(580..1131)
                     /gene="cobO"
                     /locus_tag="PK849_09365"
     CDS             complement(580..1131)
                     /gene="cobO"
                     /locus_tag="PK849_09365"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPE66373.1"
                     /translation="MMRENNPRRLFDPGLVHVYTGNGKGKTTAALGLTVRVSGHGGKV
                     AFIQFMKGWDFYGEIAGLALLPGVRHERTGTPEYVYRGKEQPVDMDEAQRGLLLAREC
                     LASGEYDLVVLDEINVAADYGLVDTDDVVQAVEERASHVEVVLTGRSAPPAFLAMADL
                     VTEMREVRHPFTKGIASRKGADY"
     gene            1244..2404
                     /gene="truD"
                     /locus_tag="PK849_09370"
     CDS             1244..2404
                     /gene="truD"
                     /locus_tag="PK849_09370"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HPE66374.1"
                     /translation="MKIKVNPEDFQVEEVLSVPLLEKKTSYRVYRLRKRNWNTVDALA
                     DIARASGVSLKELHWCGRKDRSALTTQYFSTTSQADMTFRSTNVSTEFAGFADSELGP
                     SSIAGNRFRVVLRDMVPEEAGAVSNRLESLRKHGFANYFDDQRFGDVENGGFIGEMLV
                     KRRFKAAVKRIFTACHSEAPAETRKRKKALADRWGRWSDVATLCPEPQLKAMLEALMS
                     GGGPIGSLREVPREEMGQYFSSYQSFIWNLVLAEVIKNRECRLFEIPCRTGSLPQYDE
                     LSTDDLAFLSELQIPTAAADMPPMPGETGAILSRILFERGVRLSKFRLPEIPQAYFAS
                     FARPAVVVPGEMKWEEAPDGLFSGKLMLTLGFFLPRGSFATMLIKSISGNPE"
     gene            2429..3364
                     /locus_tag="PK849_09375"
     CDS             2429..3364
                     /locus_tag="PK849_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPE66375.1"
                     /translation="MKKVPALREGDTVAFVSPSSQSTLESVEKAVSVAEDMGFHVKVA
                     KSCYCSEGYHAGPDALRADDVNRMFLDPEVKGIFCVRGGDGATRLPPLIDIEGAFKNP
                     KVFLGYSDITILHQMFNRPGGFCTFHGPMPASEFTKDTFPGYVRDSLLRAVCSTEPLG
                     EIKPYEGAAPMETLVPGNAEGELIGGNLALVCALLGTPWEIDTRGRMLVLEDTGEPVY
                     RIDRMLTQLRLAGKLDAAAGIVLGQFTQIQPRKPKDSFTVPEVFEAVVAPVGRPTLLN
                     GCFGHDVKKATLPLGARALIDGAASTLTVVEAGVY"
BASE COUNT          780 a         1065 c         1096 g          756 t
ORIGIN      
        1 tcttgaatcc tttaccactg gcggattgtc cctctccgat ggaataagct atggtttcgg
       61 acccagcata tcgtggccca tattccacgg cggcgcgata cggagcaata tcaaaatcca
      121 gacggcgaaa caggagcagc tcctggcggc ttatgagcag acagttctga acgctgttgc
      181 ggaagtgcgc aatgcactga cttcggaaac gcaggaacgg gagcgcaacc agtcgctccg
      241 gcgtggcgtc gatgcggcgc gaacggcgtt tgagatcgcg gaggacaaat acaaaaacgg
      301 tctcacggat ttcaacaacg tgattggcgc ccagagcgcc ctgcttactc ttgaggaaca
      361 gtacgcgata agcgaaggac agatgctctc gaacattgtg cgcatattca aggtgctggg
      421 cggaggatgg gcacccctgg cagcaggtgg tttcccgcag cccgtcgacg gagatacggc
      481 gaagaccacg gaatcgcaga tctcgagcga aagccggagt taccttgaac agatacgcca
      541 ggaactgaaa gactaaggaa gaaatattgt ccttaccctt taatagtcgg cgccctttcg
      601 cgatgcgatc cccttcgtga aggggtgccg gacctcccgc atctccgtca cgagatctgc
      661 catggccagg aacgcggggg gcgccgacct gcccgtgagg acaacttcca cgtgagatgc
      721 ccgttcctcg acagcctgaa cgacgtcatc ggtgtccaca agcccgtaat cggcggccac
      781 gtttatctcg tccaggacga caagatcgta ttcgcccgat gccaggcact cccgggccag
      841 gagcagacct ctttgtgcct cgtccatatc cacaggctgc tccttgcctc tgtaaacgta
      901 ttcgggggtg ccggtcctct cgtgacggac gcccggcagc agggccagtc cggcgatctc
      961 cccgtagaaa tcccacccct tcatgaactg gatgaaggcc accttgcctc catgccccga
     1021 aacccttacc gtcagcccca gcgccgccgt ggtcttgccc ttgccgttgc ccgtgtagac
     1081 atgcacgaga ccggggtcga agagtcgcct tgggttgttt tcgcgcatca ttatctgctc
     1141 ctccttccgg tgtctgaaac cgtggtctat gggaatgacg gaaaatacgt acaatggttt
     1201 atccttgttc catggatcaa aatcgcgcgc tggaggaata ctgttgaaga tcaaggtcaa
     1261 tcccgaggat ttccaggtgg aagaggtgct gtcggtcccc ctgctggaga agaaaacctc
     1321 ttaccgagta taccgccttc gcaaaaggaa ctggaacacg gtggacgccc ttgcggatat
     1381 cgcccgggcg agcggagtat ctctgaagga gctgcactgg tgcggcagga aggaccgctc
     1441 cgcactgacg acgcagtact tctcgacgac ctctcaggcg gacatgacct tcagaagcac
     1501 caacgtttcg accgaattcg cgggctttgc agactcggag ctgggaccct cttccatagc
     1561 gggcaaccgg ttcagggtcg ttctgaggga catggtccct gaggaggcgg gagccgtttc
     1621 aaacaggctc gaatctctga ggaagcatgg ctttgcgaac tatttcgacg accagcgctt
     1681 cggagacgtc gaaaacgggg gatttatcgg cgaaatgctc gtcaagcggc gtttcaaagc
     1741 cgccgtaaaa aggatattca ctgcctgcca ctccgaggcc cctgcggaga cgagaaagcg
     1801 caaaaaagcc ctggcggaca ggtggggaag atggagtgac gtggcgacgt tatgtccgga
     1861 accgcaacta aaggcgatgc tggaagccct gatgagcggc gggggcccca tcgggtcgct
     1921 cagggaagtg ccgcgtgagg agatgggaca gtacttctca tcctaccaga gctttatctg
     1981 gaacctggta cttgcggaag tgataaaaaa ccgggagtgc aggcttttcg agatcccatg
     2041 ccggacggga tcactccccc agtacgatga actctccact gacgatctgg catttctttc
     2101 ggagctgcag atcccgacgg cagccgccga catgccgcca atgccggggg agacaggagc
     2161 aatactctcc cgcatcctgt tcgaaagggg cgtgaggctt tcaaagttcc ggctgccgga
     2221 gatcccccag gcttatttcg catcctttgc ccgtccggcg gtggtcgtgc ccggtgagat
     2281 gaagtgggaa gaggcccccg acgggctctt ttccggaaag cttatgctga ccctcgggtt
     2341 ctttctcccc aggggctcct tcgcgacgat gctgataaaa tccataagcg ggaatcccga
     2401 ataaaagaag agcgggggag tgatttccat gaaaaaggta ccggcacttc gcgaagggga
     2461 cacggtggcc ttcgtctccc cttcgagcca gtcgaccctt gaatcggtcg aaaaagccgt
     2521 atctgtcgcg gaggacatgg gtttccatgt caaggtggcc aaaagctgct actgctccga
     2581 ggggtatcat gcgggcccgg acgctctgcg cgccgatgac gtgaacagga tgttcctgga
     2641 cccggaggtg aaggggatat tctgcgtccg gggcggggac ggggccacaa ggcttccgcc
     2701 ccttatcgat attgaaggtg ccttcaaaaa ccccaaggtc ttcctgggct acagcgacat
     2761 aacgatcctt caccagatgt tcaaccgccc cggcggcttc tgcacctttc acggcccgat
     2821 gcccgccagc gaattcacaa aagacacctt ccccggctac gtgcgggaca gcctcctcag
     2881 ggccgtctgc tcaacggagc cgttgggtga gataaaaccc tacgaaggag ccgcaccgat
     2941 ggagaccctc gttccgggta atgccgaagg cgaactcatc ggtgggaacc tggcactggt
     3001 atgtgccctg ctggggactc cctgggagat cgacacgcgg gggcgcatgc tggttctcga
     3061 ggatacgggc gaaccggtct accgcatcga caggatgctc acccagcttc gcctggcggg
     3121 caagctggac gccgcggcag ggatcgtcct gggtcagttc acccagatcc agccgagaaa
     3181 accgaaggat tccttcacgg tccccgaggt attcgaggcg gtcgtggccc ctgtcggcag
     3241 gccaaccctt ttgaacggct gtttcggcca tgacgtgaag aaggcgaccc ttcccctcgg
     3301 tgccagggcc ttgatcgacg gcgctgcatc aaccctgacg gtcgtggaag cgggggtgta
     3361 ttaggtaacg cgtgactcgt tacgcgtgac tcgtgactgg taagaccaaa aactggatga
     3421 agtccccgcc gaatttctcg ggaatcaact atctggagct ccttttttat tgttccctgg
     3481 ttcccaggtt cgggttttcc gagtcacgcg tcacgagtta cgcgttacgc tttataaaaa
     3541 atgacttcct ttgcgatgct tcttctggta acggcggcgt tccttcatgc gggttggaac
     3601 ctggtggcaa agcgcgtctc cggcggcgcg gcctttacat ggcttttctc ggtgctttcg
     3661 gccttgctgt tgttcccttt cgccatcttc gcgacat
//
LOCUS       DAOPKU010000290         3696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27714_length_3696_cov_4.633617, whole genome shotgun sequence.
ACCESSION   DAOPKU010000290 DAOPKU010000000
VERSION     DAOPKU010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27714_length_3696_cov_4.633617"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..421
                     /locus_tag="PK849_09380"
     tRNA            348..421
                     /locus_tag="PK849_09380"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:380..382,aa:Gln,seq:ctg)
     gene            431..506
                     /locus_tag="PK849_09385"
     tRNA            431..506
                     /locus_tag="PK849_09385"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:465..467,aa:Glu,seq:ctc)
     gene            681..1499
                     /locus_tag="PK849_09390"
     CDS             681..1499
                     /locus_tag="PK849_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPE66376.1"
                     /translation="MVFCFMNGVFLDGDQATLPVSDLALQRGVAVFDTVRSYDGRPFA
                     LKAHLERLSNSARLARIAEPLSAEEIGDIVREGLSRIEGDGLAKIFLTGGDVEEFGSF
                     PSPRFLALFSPVAPIPEEFFTKGIALSLLHEERQLFRIKSINYMNAYRNHLPGTFEAL
                     HCVDGEITESATSSFFGVAKDNLITAPDHRVLRGITREILIDLARKEGYNVEYRCLTL
                     DELPDLTEAFITGSVKEVTPVTRIGDTIIGGGAPGAVTKRMYELFKESVPNHLG"
     gene            1560..2120
                     /locus_tag="PK849_09395"
     CDS             1560..2120
                     /locus_tag="PK849_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPE66377.1"
                     /translation="MGNGVYIVAAIAAGVLVYAVYIYNTLVRRKASVENGWSQIDVQL
                     KRRYDLIPNLVETVKGYAAHEREVFERIASLRSAALSATSPEQAAPLQNQLTGALRSL
                     FAVSENYPELKANQNFLALQEELASTENKIAFARQYYNDAVKAFNIAIDVFPNNLISR
                     RLGYEPRAMWAADDASELQSVQVTWR"
     gene            2125..3042
                     /gene="htpX"
                     /locus_tag="PK849_09400"
     CDS             2125..3042
                     /gene="htpX"
                     /locus_tag="PK849_09400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HPE66378.1"
                     /translation="MVSFEGLIAHNKRLSILLFFLLLLLLGGLGASVSYWWGGEWYTG
                     VIVATAVAVIYFLFSWFAGASMILSMSGAREIRHEDAPQLWNVVEELSIAGGLPMPKV
                     YLIDDSAMNAFATGRDPEHASVAITRGLMEKLNREELQAVMAHEMSHVRNFDIRFAML
                     MAVMVGSIVMICDMVFRSVFFGRRRSSRSSDGGKGQAVMAVVALVLAILAPLLATIIQ
                     LAVSRQREYLADASAVELTRNPAGLVSALRKLSGDPNELQRASRGTQHLYIVNPLRKM
                     KQGKNSLFSTHPSLESRIERIEGLSLARA"
     gene            3039..3320
                     /locus_tag="PK849_09405"
     CDS             3039..3320
                     /locus_tag="PK849_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPE66379.1"
                     /translation="MTLIRKRFLVMGTVQGVGFRHFAARMALKFGVTGWVRNLPDGAV
                     EVHAQGEREAMDKMEQWLRKGPSSATVTRLVVLETPLKEGESSFAIRHH"
     gene            3361..>3696
                     /locus_tag="PK849_09410"
     CDS             3361..>3696
                     /locus_tag="PK849_09410"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HPE66380.1"
                     /translation="MTAFLLLFSVGTAGYLVFKKFALPVPAILGSLFFVSVLNLAGFF
                     PEVQLQWISRYSNIVIGSYVGLRVNKQSLTLLRELPVPALIVSLSMLFLSLAGGSVLY
                     MMSDLSMKTA"
BASE COUNT          749 a         1023 c         1071 g          853 t
ORIGIN      
        1 gagcattctc aacacgtcct cgggttccat gagatcaatc atccgacgga gctgttgttt
       61 ttcttcagcg gctgcgacag gcatctgcat cacctccgca gtcattatat cctaaggaag
      121 ctctgattaa gggatttttc ctcttcattg agctcgattg catcaagctc ctacgagagt
      181 atctcagctt tattcagagg ttccttaaat caaaacgtca acactaattt tttgcttttt
      241 actttgttca aatactaagt ctttttcatt tcctctcccc cccttgtcaa atgccggatt
      301 tctggtatca tgtaccccgc acacgagaaa atgcgcgtgc ccgaagttgg ggaatcgtcc
      361 aatggcagga cgcctgactc tggatcaggt aatcttggtt cgaatccagg ttccccagcc
      421 agcaagaaat ggccccatcg actagtggcc taggtcgtaa ccctctcaag gttaaaacac
      481 gggttcgaat cccgttgggg ctaccaaaat aaaccgtacg aatgtgcggc gttatacatt
      541 gaaagaccgc ttgaacgagc ggtctttttt tattcttctt tactgaaaca accgattcgg
      601 cattgagctt ctcgcgtcct gatgttagga tagtactatc aaccgaacat attttttaca
      661 taatcgaggt gctttccaac atggtatttt gtttcatgaa cggggtgttt ctggatgggg
      721 accaggcaac acttcccgtt tccgaccttg cactgcagcg cggagtggcg gttttcgaca
      781 ccgtccgaag ctacgacggc cgtcctttcg ctctcaaggc ccatctggaa aggctttcga
      841 attcggcgcg gctggcgcgg atcgcggagc ctctctctgc ggaagagatc ggggatatcg
      901 tccgggaggg gttgtcgcgc atcgaagggg atggcctggc gaagatcttc ctgaccggcg
      961 gggacgtcga ggagttcggt tcgtttcctt cgccgcgatt cttagcgctc ttctcaccgg
     1021 tcgctcctat tccggaggag ttttttacga aggggatcgc gctttccctt ctgcacgagg
     1081 agcgtcagct tttcaggata aagagcatca actacatgaa cgcctacagg aaccaccttc
     1141 cggggacgtt cgaggcgctc cactgcgtgg acggagagat tacggagtcc gctacgagca
     1201 gcttcttcgg cgtagcgaaa gacaacctga tcacggctcc ggaccatcgc gtgcttcgcg
     1261 gcattacgcg ggagattctc atagatcttg cgcgaaaaga ggggtacaac gtggagtacc
     1321 gctgcctcac gctcgacgaa ctgcccgatc tgacggaggc cttcatcacc ggaagcgtaa
     1381 aagaggtgac gccggtgacg agaataggcg acacgattat cggcggcggc gccccgggcg
     1441 cggttacgaa gcgcatgtac gaactcttca aggagagcgt tcccaaccat ctcggctgat
     1501 tcgcgctggt gtataatact tttgttgaag aacgcaatcc ggaagggagg ctgtcgtcca
     1561 tgggaaatgg cgtctatatt gtcgctgcga ttgctgcagg tgttctcgtg tacgccgtct
     1621 atatctacaa cacgcttgtg cgcagaaagg cgagcgtcga gaacggatgg agccagatcg
     1681 acgtccagtt gaagcggcgg tacgacctta tcccgaacct tgtggagacg gtgaaggggt
     1741 acgccgcgca tgagcgggaa gttttcgagc gcatcgcctc tttacgaagc gccgcgcttt
     1801 ccgcgacctc tcccgaacag gccgctccgt tgcagaatca gctcaccggc gcgctgcgtt
     1861 cactttttgc ggtctcggag aactaccccg agctgaaggc gaatcagaac tttctcgcgc
     1921 ttcaggagga gctggcctcc acggagaata aaatcgcgtt cgcccgccag tactacaacg
     1981 acgcggtaaa ggcgttcaac atcgctatcg acgtcttccc gaacaacctg atctcgcgga
     2041 ggctggggta cgagccgcgc gcgatgtggg cggcggacga cgcttccgag ttgcaatccg
     2101 ttcaggtcac ctggaggtga gagcatggtc agcttcgagg ggcttatcgc gcataacaag
     2161 cgtctgagca ttttgctctt cttcttgctg ctgctgctgc tcggcggtct gggagcttcc
     2221 gtcagttact ggtggggcgg cgaatggtac acgggggtga ttgtcgccac ggcggtcgcg
     2281 gtaatctact tcctgttctc gtggttcgcg ggggcctcca tgatcctctc gatgagcggc
     2341 gcgagggaga tccgtcatga agacgccccg cagctctgga acgtcgtgga ggagctctcc
     2401 atcgcaggag gactcccgat gccgaaggtc tatctgatcg atgacagcgc gatgaacgcc
     2461 ttcgccaccg ggcgggatcc tgagcacgcg agcgtggcga tcacgcgcgg gctgatggaa
     2521 aagctgaacc gggaggagct tcaggctgtc atggcgcacg aaatgtcgca tgtgcggaac
     2581 ttcgacatcc gcttcgccat gctgatggcg gtgatggtgg gctccatcgt catgatctgc
     2641 gatatggttt tccgcagcgt cttcttcggc cgtcgccgca gcagccgttc aagcgacgga
     2701 ggcaagggac aggcggtgat ggcggtcgtg gcgctcgtgc tcgcaattct tgcgccgctc
     2761 ttggccacaa tcatccagct cgccgtcagc cgtcagcggg agtacctcgc ggacgcctcg
     2821 gcggtcgaac tgacgcgcaa tccggcgggc ctggtatccg ccttgagaaa gctgtccggc
     2881 gacccgaacg aactccagag agcgagccgc ggaacgcagc acttgtacat cgtcaacccg
     2941 ctccggaaaa tgaaacaggg aaagaacagc cttttcagca cgcatccgtc ccttgaatcc
     3001 agaatcgagc gcatcgaagg gctttcgctc gcacgagcat gacattgatc cgcaagcgtt
     3061 ttctggtgat gggaacggta cagggcgtag ggttccgcca ctttgcggcg cggatggcgc
     3121 tgaagttcgg cgtgaccgga tgggtccgga atctgccgga cggcgcggtc gaagtgcacg
     3181 cgcagggcga gcgtgaagca atggacaaga tggaacagtg gctccgcaag ggaccgtctt
     3241 ctgcaaccgt gacgcgcctg gtcgtgctgg aaacgccgct gaaggaaggc gagagctcgt
     3301 tcgctatccg tcaccactaa aaaattcgca ccgtgcggtt cggaggttcg gttttctctt
     3361 atgacagctt ttctcctgct tttttcagtc ggtaccgccg gctatctggt attcaaaaag
     3421 tttgctcttc ccgtaccggc gattctcggc tcgctctttt ttgtgagcgt ccttaatctt
     3481 gccggttttt ttcctgaagt gcagctgcaa tggatctccc gatacagcaa catcgttatc
     3541 ggaagctacg taggattgcg ggtgaacaaa cagtctctca cgctgctccg ggagcttccc
     3601 gtaccggcgc tcatcgtttc gctgagcatg ctcttcctct ccctcgcggg aggaagcgtc
     3661 ctctatatga tgagcgatct ctccatgaaa acggcc
//
LOCUS       DAOPKU010000291         3691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27775_length_3691_cov_3.886414, whole genome shotgun sequence.
ACCESSION   DAOPKU010000291 DAOPKU010000000
VERSION     DAOPKU010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27775_length_3691_cov_3.886414"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..991)
                     /locus_tag="PK849_09415"
     CDS             complement(239..991)
                     /locus_tag="PK849_09415"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPE66381.1"
                     /translation="MLVFGHRGASGYAPENTLPAFELGIRQGCEGFEFDVQLTKDGVA
                     VVHHDWTVDRTTTGSGAIPDLTFEEIRAFDAGKWFSEEFTGTTVPRLEELFELVPDTM
                     PLNVELKSRPHDRPGLEETVAALLERYNRVENTIISSFNHKSLKTMRRLNPAFRLGIL
                     YEGVLLSLFSYAKRQELDLYSLHPCHDYVSAELVEKAHARGIKVACWTVNTKERAREL
                     EAAGVDVIITNYPDRCLQEAPKGKKGKKKAGK"
     gene            complement(1059..2342)
                     /locus_tag="PK849_09420"
     CDS             complement(1059..2342)
                     /locus_tag="PK849_09420"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE66382.1"
                     /translation="MEFLPVIVVFVLYFSGIPIAFALFGAALSYFTFINTGSPVDLIL
                     QRFVTSTASFPLLAVPFFIMAGSIMNYAGISSRLMKMADVLTGHMTGGLAQVNVVLST
                     LMGGVTGSANADAAMQSKILVPEMEARGYDRAFSAAITAASSAIAPVIPPGINLIIYA
                     LIARVSVDKIFLAGYVPGLIMCCTLMIAVSIVSKKRGYKPSRERRATAREMLRQVRES
                     SWALFMPLGIILGLRFGMFTPTEAGAIAVLYCSLVGFLVYRELRPVHFITILKDTVYG
                     TSTVLLIIVAASVFGYYMTWERIPQTVAALLLDIAHNKYTMLLVINIMLLILGMFIEG
                     GAAMIILAPLLVPVVVGLGVDPVHFGLVIIVNIMIGGVTPPFGSMMFTTCSITGVAIP
                     DFIRESIPFIIALLVALLIVTYFPALTLFLPHLLY"
     gene            complement(2348..2845)
                     /locus_tag="PK849_09425"
     CDS             complement(2348..2845)
                     /locus_tag="PK849_09425"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE66383.1"
                     /translation="MKKIVHTLFSYAEEIVSAFFISITVCMVIVNVFFRYVLNMGIFW
                     SEEVATISFVWSVFIGAAACHKRKMHISIDLLTKLAPDFLKKPLRLMAHTIMIILNGY
                     IVYLSVIFVRASSIKPTAVLGISSSYVSSALLVGFGLMTLHSAVFFFEELRSGALPQE
                     QAAQG"
     gene            complement(2952..>3691)
                     /gene="dctP"
                     /locus_tag="PK849_09430"
     CDS             complement(2952..>3691)
                     /gene="dctP"
                     /locus_tag="PK849_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE66384.1"
                     /translation="FISVEDPSYVGDYVPDFIALVGPMLYNSYDEYVEIIKTDLVQDM
                     IKRAEEKGIKILALEYVFGFRNVITNKVVKTPEDLKGVKLRTPGSQLFIQTLTAMGAT
                     VTPLPWAETLPGVQQGIVDGIEGSEFTNIGTKVYELLKNVARTQHFLGTCGVYISTQV
                     WEKIPEEYRAIIAEEFAKGALEMVEIGKRDLGDVTAKLESYGVKFNEVDKKAFTEATK
                     SIYETFPGLTPGIYDKLQAELEKIRAK"
BASE COUNT          771 a         1060 c         1100 g          760 t
ORIGIN      
        1 tcaactttac ttttcgcttc cgtccccgct ctttcccgcc gccgctcagg cggcttgaag
       61 agcgcgccgt ttcaaagacg cgaggggtat aataccactt ttgcagaggt tgtcaacagc
      121 gtttttttga aaatccgttt tcttaataga gagtcttcga ccgtccctcc cgctcccatt
      181 caagacgcac gcgtcctgcg ctccgcgaga gcgcaggacg gaggggctcg gcctgttgct
      241 acttacccgc ctttttcttc cctttcttcc ccttgggcgc ctcctgcagg catctgtcgg
      301 ggtagttggt gatgatgacg tctacccctg cggcctccag ctcgcgggca cgctccttcg
      361 tgttgaccgt ccagcacgcg actttgatcc cgcgggcgtg cgccttctcc accagctccg
      421 ccgagacgta gtcgtggcag gggtgcaggc tgtagagatc caactcctgg cgttttgcgt
      481 aggagaacag ggagagcaga accccttcat agagtatccc caggcgaaac gcgggattca
      541 gcctgcgcat cgtcttcagg cttttatggt tgaaggagga gatgatcgtg ttctcaaccc
      601 ggttgtaccg ctccagtagt gcggcgactg tttcttcaag cccgggccgg tcgtgcggtc
      661 tgctcttcag ctccacgttg agcggcatcg tatccggaac cagctcgaac agctcttcca
      721 ggcgcggaac agtcgtccct gtaaactcct ccgagaacca tttccccgcg tcgaacgcac
      781 gaatttcttc gaatgtgaga tcgggtatgg cgccgctccc cgtggtcgtg cggtccacgg
      841 tccagtcatg atgaacgacg gcaacgccgt ctttggtcag ctgcacatcg aattcaaaac
      901 cctcgcaccc ctgccggatt ccgagttcaa aagccggcag agtattctcc ggggcgtacc
      961 ccgaggcccc cctgtgtccg aagacaagca ctgaaacccc ttctttctat aggtagagag
     1021 tatgtaagga gcgcgccgaa gcgcgctcct cccgtcgttc agtagagcag atgcggtaaa
     1081 aagagcgtga gcgccgggaa ataggtcacg atcagcaacg ctacgagcag tgcgatgatg
     1141 aacgggatgc tctcacgtat gaagtccgga atggcgacgc cggtgatcga gcaggtggtg
     1201 aacatcatcg acccgaacgg gggcgtcacg ccgccgatca tgatgttcac gatgatgacc
     1261 agtccgaagt ggaccggatc gacgccgagg ccgacgacga ccggcaccag gagcggcgcc
     1321 aggatgatca tggccgcgcc cccctcgata aacatcccga ggatgaggag catgatgttg
     1381 atcacgagga gcatcgtgta cttgttatgc gctatgtcga gcagcagcgc ggcgacggtt
     1441 tgcggtattc gctcccaggt catgtagtag ccgaagaccg aggccgcgac gatgataagg
     1501 agcacggtgc tggtgccgta gacggtatcc ttcaagatgg tgatgaaatg aaccggccga
     1561 agctcgcggt agacgaggaa accgacgagc gagcagtaga gcaccgcgat tgcgccggcc
     1621 tcggtggggg taaacatgcc gaagcgcagt ccgagaatga tgccgagcgg catgaagagc
     1681 gcccaggagg actccctgac ctgccgcagc atctctcttg cagtggcccg ccgctcacgc
     1741 gagggtttgt accctctctt ctttgaaacg atgctcaccg caatcatgag agtacagcac
     1801 atgatgaggc cggggacgta ccccgccagg aagattttgt cgaccgagac gcgcgcgatc
     1861 agggcgtaga tgatcaggtt gatccccgga ggaatgacag gcgcgattgc cgaagaggcc
     1921 gccgtgatgg ccgccgaaaa ggcgcggtcg taccccctgg cctccatttc gggaaccagt
     1981 attttgctct gcatggcggc gtcggcattt gcagaccccg tgacgcctcc cataagcgtg
     2041 ctgagcacca cgttgacctg cgccagccct cccgtcatat gcccggtaag gacgtcggcc
     2101 atcttcatca ggcgggagct tatccctgcg tagttcatga tcgatcccgc catgatgaag
     2161 aagggtacgg ccaggagcgg gaaggaggct gtgctcgtga caaagcgctg gaggatcagg
     2221 tcgacggggc tgcctgtatt gatgaaggtg aaataggaca gcgccgcgcc gaagagagca
     2281 aacgcaatgg ggatgcccga aaagtagagc acgaagacga cgatgacagg gagaaattcc
     2341 atctgcatca cccctgcgca gcctgttcct gaggtagagc gccgctgcga agctcttcga
     2401 agaagaagac ggcggaatgg agcgtcatca gtccgaagcc gaccaggagc gccgagctca
     2461 catagcttga ggagattccc agaacggcgg tcggttttat cgaagaagcc cggacgaaga
     2521 tcacgcttaa atagacgatg tacccgttga ggatgatcat aatcgtgtgc gccatcaggc
     2581 gaagcggctt cttcaggaag tccggggcaa gtttcgtcag aaggtcgatg ctgatatgca
     2641 tcttgcgctt atgacacgcc gcggccccta tgaagacgct ccagacgaaa gagatggtcg
     2701 cgacctcttc ggaccagaag atccccatgt tgagcacgta acggaaaaag acgttgacga
     2761 tgaccatgca gacggtaatg ctgatgaaga atgcgctgac gatttcctcc gcatagctga
     2821 agagtgtatg tacgattttt ttcatggtcg ataacctctc gtcgagtgaa tcagcggcag
     2881 ggagagaaac cacgttccct ctccctgccg cgccgtacgt ttatttcatt cgcgtgaagg
     2941 aaatccggcg actacttcgc ccggatcttc tccagctccg cctgcagctt gtcgtagatg
     3001 ccgggcgtca ggccggggaa cgtctcgtag atgctcttcg tcgcctcggt aaaggccttc
     3061 ttgtcgacct cgttgaactt cacgccgtac gattcaagct tcgcagtgac atcgccgaga
     3121 tctctcttgc cgatctccac catttcaagc gcgcccttcg cgaactcctc ggcgataatc
     3181 gcccggtact cctcggggat cttctcccat acctgcgtgg agatgtagac gccgcaagtg
     3241 ccgaggaagt gctgggtgcg ggcgacgttc ttcagcagct cgtacacctt ggtgccgatg
     3301 ttggtgaact cggagccttc gataccgtcg acgattccct gctgcactcc cggaagagtc
     3361 tcggcccagg ggagcggggt cacggtcgct cccatcgctg tgagcgtctg gatgaagagc
     3421 tggcttcccg gggtgcggag cttcacgccc ttcaagtctt cgggcgtctt gacgaccttg
     3481 ttggtaatga cgttgcggaa gccgaagacg tattcaagcg caaggatctt gatccccttc
     3541 tcctcggccc tcttgatcat gtcctggacc agatcggtct tgatgatctc gacgtactcg
     3601 tcgtaggagt tgtagagcat cgggccgacc agcgcgatga aatcgggaac gtagtcgccg
     3661 acgtaggacg ggtcctcgac ggagatgaag t
//
LOCUS       DAOPKU010000292         3685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27817_length_3685_cov_4.887052, whole genome shotgun sequence.
ACCESSION   DAOPKU010000292 DAOPKU010000000
VERSION     DAOPKU010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27817_length_3685_cov_4.887052"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="PK849_09435"
     CDS             complement(<1..1276)
                     /locus_tag="PK849_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HPE66385.1"
                     /translation="MDLVVRNARLRGESGTKDIAVRDGLVAAIGERLAVQAGREIDAG
                     GNLASAPFIDPHLHLDAALSVGDPRYNESGTLLEGIAIWGERRPSLQKNEVLRNAREA
                     VLWEVANGVQFIRTHADTTDSALVTVEALVELREEMKDVAEIQVVAFPQDGVFTFPEG
                     EANLRRALDMGCDCVGAIPHIEFTREDGVRSVELAFELAEQYGRPVDIHCDETGDDQS
                     RFVEVMAKLAIATGMGSAVTASHTTAMHNYNNDYALKLMGIFRRAKMNFITAPFDNSV
                     LQNRTDGYPRRRGHTRVDELDAAGVNVSIGHDSIMDPWYPMGRGSMLAAANLLMHTAH
                     MSGREQISRLFDMITDNSAVTMGVRDRYGVTPGAPADFVILDAPDEFEALRLQSECLF
                     VVRGGTVVCETAPAKRRLRRSGAVREVDFKLPA"
     gene            1610..2602
                     /locus_tag="PK849_09440"
     CDS             1610..2602
                     /locus_tag="PK849_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HPE66386.1"
                     /translation="MKKFVALLALCLLVLFAGTAAADVKEYKDFKLAAVFQTAIEEPW
                     DGAIHQACLKVQKETGITYEFAEKVGAADFERVLREYAERGFDLIVGDAFLAGEEPSR
                     RVAKEYPEVAFAFGSEFTFQEPNYSVFDNWIHEPSYLCGVIAGRMTKSNTLGIVAAIP
                     IAEVNRLVHAFKEGALSVNPAVKVKVAYIGSWFDPPKAKEAALAQIESGVDIIFAERF
                     GVFEAAKEHKLLAFGNMLDQHDLAPEVVVTGPVWDMYPTVLHSVDAVRKGEWKAENLR
                     EWSMMAKGGAFLAPFHEFEQKLPAELMKEVRDLEAKILAGEFTVPVIETELKTD"
     gene            2678..>3685
                     /locus_tag="PK849_09445"
     CDS             2678..>3685
                     /locus_tag="PK849_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE66387.1"
                     /translation="MSRPDTPRTHLEERTPVVEMRSIRKTFLDVVANSDVDFTLYKGE
                     ICALLGENGAGKTTLMNILFGYYAADSGEIRIGGEKVSLSSPRDAITRRIGMVHQHFT
                     LVPSQTVLENTVVGSCGGFFLDMGKARRKLLELQRRFGLHVDPDAPVWTLPIGGQQKV
                     EILKALYRDARILILDEPTAVLAPLETMELFGTLRALAAEGCSVVFISHKLYEVMEIA
                     DRVVVLSKGSVAAERETRDTDERELANLMVGRELAVQRHSGSQRRGDPTLEIRSLSVR
                     NDKELEAVKELSLAIRSGEILGMAGVSGNGQRELAEALFGLRVPDGGDVLLGGEPLPP
                     GR"
BASE COUNT          678 a         1154 c         1186 g          667 t
ORIGIN      
        1 tcgccggcag cttgaaatcg acctcgcgaa cggcgccgct ccggcgcaac cgccgctttg
       61 caggcgcagt ctcgcacacc accgtaccgc cgcgcaccac gaagaggcat tcgctctgga
      121 gacgaagcgc ttcgaattcg tcgggagcgt cgaggataac aaaatcggcc ggagcgcccg
      181 gtgtgacgcc gtagcgatcc cggacgccca tggtcacggc ggagttgtcc gtgatcatgt
      241 cgaaaagtcg gctgatctgc tcgcgcccgc tcatgtgcgc cgtgtgcatc agcaggttcg
      301 ccgcggcgag catggagccc ctccccatcg ggtaccaggg gtccatgatg gagtcgtgtc
      361 cgatggagac gttgacgccc gcggcgtcca gttcgtccac ccgcgtgtgc cccctgcggc
      421 gaggatagcc gtccgtgcgg ttctggagca cggagttgtc gaacggcgcg gtgatgaagt
      481 tcatcttcgc ccgccggaag atccccatca gcttgagcgc gtagtcgttg ttgtagttgt
      541 gcatcgccgt ggtatggctc gccgtgacgg cgcttcccat ccccgtcgcg atggcgagct
      601 tcgccatgac ctcgacgaag cgcgactggt cgtctccggt ttcgtcgcag tgaatatcca
      661 ccgggcggcc gtactgctcc gccagctcga aggccagctc gaccgagcgg acgccgtcct
      721 cgcgggtgaa ttcgatgtgc gggatggcgc cgacgcagtc gcaccccata tcgagggcgc
      781 ggcgaaggtt cgcctcacct tccgggaagg tgaagacgcc gtcctgcgga aaggcgacca
      841 cctggatctc cgcgacgtcc ttcatctcct cgcgcagctc gacgagcgcc tccaccgtaa
      901 cgagggcgga atcggtggtg tccgcgtggg ttctgatgaa ctgcacgccg ttggcgacct
      961 cccagagcac ggcctcgcgc gcgttgcgga gcacttcatt cttttgaaga gaggggcggc
     1021 gctcccccca gatggcgatg ccttcgagga gcgttcccga ttcgttgtaa cggggatctc
     1081 ccacgctcaa ggcggcgtcg aggtgcagat gagggtcgat gaagggcgcc gacgccaggt
     1141 tgcccccggc gtcgatctca cggccggcct gaacggcgag ccgttctccg atcgcggcca
     1201 ccaggccgtc acgaaccgct atatccttcg ttcctgactc tccgcgaagc cgcgcattgc
     1261 gtaccacaag gtccattctc tcttctcccc gccgatttga tttatgcaat cgtttgcatt
     1321 aaaatagtac cacaatcgct cttccctgtc cactgccgac aggaagagcg aatattcacg
     1381 tcacccgtcg ccattgacag aagaacctct cgttgattga atgattgaaa atcatcgaga
     1441 gtgcagaact tctgataaaa tgggaattgg cgcgattagg aaaaaccgcg gaggtcaagg
     1501 aggtgacggg gcggaactct tccatgcgga acgtgggaat tcatggtctg tacgacggcg
     1561 tcgggcatcc gcccgtctgc cggatactct gaacgggagg tgcatttgtg tgaagaaatt
     1621 tgtagctctg cttgctcttt gtctgctggt tctcttcgcc ggaacggcgg cggccgatgt
     1681 gaaggagtac aaggacttca agctcgcggc cgtcttccag acggcgatcg aggagccgtg
     1741 ggacggcgcg atccatcagg cgtgcctgaa ggtgcagaaa gagaccggca tcacctatga
     1801 gttcgcggag aaggtcggcg cggcggactt cgagcgcgtg ctccgcgagt acgccgagag
     1861 agggttcgac ctgatcgtcg gcgacgcatt cctcgcgggc gaggagcctt cgcggcgcgt
     1921 ggcgaaggag taccccgagg tcgcgttcgc tttcggctcg gagttcacct tccaggagcc
     1981 gaactactcg gtcttcgaca actggatcca cgagccctcc tacctgtgcg gggtgatcgc
     2041 cggacggatg acgaagagca atacgctggg catcgtcgcg gccatcccca tcgccgaggt
     2101 caacaggctg gtacacgcat tcaaagaggg cgcgctctcg gtgaacccgg ccgtcaaggt
     2161 gaaggtcgcc tacatcggaa gctggttcga tcctccgaaa gcgaaggaag ccgccctggc
     2221 ccagatcgag tcgggcgtcg acatcatttt cgccgagcga ttcggcgtct tcgaggcggc
     2281 gaaggagcac aagctgcttg ccttcggcaa catgctcgac cagcacgacc tggcccccga
     2341 agtcgtcgtg acgggccccg tatgggacat gtacccgacg gtgctccact cggtcgacgc
     2401 ggtccgcaag ggcgaatgga aggccgagaa cctccgcgag tggtcgatga tggccaaggg
     2461 cggcgcgttc ctcgccccct tccacgagtt cgagcagaag cttcccgccg agctgatgaa
     2521 ggaagtccgc gacctcgagg cgaagatcct cgcaggggag ttcaccgtcc cggtcatcga
     2581 gacggaattg aagacggact gatgtttttt tggggaacgg ggggatgcct ccgttcccct
     2641 tttcgacgga aacacccgaa agggggaatg gctgccgatg agccggcccg acacaccacg
     2701 cacacacctt gaagagcgca ccccggtcgt tgagatgcgc tcgatccgga agacctttct
     2761 cgacgtcgtc gcgaacagcg acgtggactt cactctgtac aagggtgaga tctgcgccct
     2821 tctcggcgaa aacggcgcgg gaaagacgac gctcatgaac atcctcttcg gatactacgc
     2881 cgcggacagc ggcgagatcc gtatcggggg cgagaaggtc tccctctcct ccccccgcga
     2941 cgccataacc cggcgcatcg gtatggtgca ccagcacttc accctcgtcc cttcgcagac
     3001 agtgcttgaa aacaccgttg tggggagctg cggcggcttc tttctcgaca tggggaaagc
     3061 gcgccggaag ctgctcgaac tgcagcgccg attcggcctg cacgtcgacc cggacgcgcc
     3121 cgtgtggacc ctccccatcg gcggccagca gaaggtggag atcctgaagg cgctctaccg
     3181 cgacgcgcgc attctcatcc tcgacgagcc gacggccgtg ctcgctcctc ttgagacgat
     3241 ggaactcttc ggcacgctgc gcgcactggc cgccgagggg tgctcggtcg tcttcatttc
     3301 gcacaagctc tacgaggtca tggagatcgc cgacagggtg gtcgttctct cgaaggggag
     3361 cgtggccgcc gaaagggaga ctcgcgacac ggacgagcgc gagctggcga acctgatggt
     3421 gggacgggaa ctcgccgtac agcggcacag cggctcgcag cggcggggcg accccacgct
     3481 cgagatccgc tcgctctcgg taaggaacga caaggagttg gaggcggtca aggagctctc
     3541 actcgcaatc cgttccgggg agatcctcgg gatggccggg gtctccggca acggccagcg
     3601 ggagcttgcg gaagccctct tcggcctccg cgtccccgac ggcggcgacg tgcttctcgg
     3661 cggagaaccg cttcctcccg ggagg
//
LOCUS       DAOPKU010000293         3683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27833_length_3683_cov_4.248897, whole genome shotgun sequence.
ACCESSION   DAOPKU010000293 DAOPKU010000000
VERSION     DAOPKU010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27833_length_3683_cov_4.248897"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PK849_09450"
     CDS             <1..652
                     /locus_tag="PK849_09450"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPE66388.1"
                     /translation="FKVSKGIGFLTSGLIIFSVSEGIHPVSLVLTLSVSAFVVLFVEI
                     IRRQLRGESFAIWNMGGTISGIVYITIPWTFMILLRRLPGGGLLLLAMFIATWSCDVA
                     AYLIGSRWGRTLLCVNVSPNKTWEGFFGGFSAALLASVLLAFILEIPPIPFVYVGIIC
                     GFAGQLGDLAESVIKRESWVKDSGTLIPGHGGVLDRFDSTLINGLLVYVLFGVILR"
     gene            649..1827
                     /gene="dxr"
                     /locus_tag="PK849_09455"
     CDS             649..1827
                     /gene="dxr"
                     /locus_tag="PK849_09455"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPE66389.1"
                     /translation="MKKRLFVIGCTGSVGSSVLSVCRMFPEHFEIAALAACSSVDRML
                     LLAEEFSCPTLVFSDESAAVRAANELLSSGVQYTGRRVLAGEQGLLEMASHPDTDHVV
                     FASSGTGAIGALMAALSSGKEVSLANKESIVVAGPWVLPLVLRPDQIRPLDSEHNAIW
                     QCLSGEDRRFVNKIFLTASGGPFRSFSQEEMKRVTPSMAVAHPVWNMGAKISVDSATL
                     MNKGIEILEAMYLFSFSADQVDAVISPNPFIHGLVCFSDGTFHMCASAPDMGIPALSA
                     LFSPLRSPRDRKNTPVLDGMQISFEAPDEVRFPCIRLAREVARKEGAYPALLVGADEV
                     AVDRFLSGRVSFMSIPRIVESVLEEYSGPSPSTLEHALHILEEGRRMAEKVSSRLEEK
                     "
     gene            1848..2873
                     /gene="rseP"
                     /locus_tag="PK849_09460"
     CDS             1848..2873
                     /gene="rseP"
                     /locus_tag="PK849_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPE66390.1"
                     /translation="MVSLVSFLLVIGICVIIHELGHYFTARIFDVQVHEFAFGMGPVL
                     LQRQGRHTMWSFRAFPVGGFVRLAGMGEDHEEEVVEEGRGFFDKAAWKRFLILANGSV
                     SNILLAMLLTVFFLSGHGVINLESTEIGEIMEGYPAQTAGILPGDAIVAVNDVPVSSW
                     REMSESIRTTASEGPVLFSVLRKGETLSLTVTIPPDAQGIPLLGIRPAMRRYSVLEAF
                     ASAFTYTVDMTFEMVRGIIRWITGVEKVDVTGPVGIASMAGDAARRGMWPFVTFLALI
                     NLNLGLLNLFPFPALDGGRLIFTAGEMLLRRRLPARIENFIHMSGFVLLLLLIVYVTW
                     QDILRFF"
     gene            2889..>3683
                     /locus_tag="PK849_09465"
     CDS             2889..>3683
                     /locus_tag="PK849_09465"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPE66391.1"
                     /translation="MSSRQLALRDLTIGGEAPVRVESMLKIPLSLPEDCLAQCFSLRK
                     VGCELVRVAFPSADLRGNLEWLNDRSPIPLMADIHFDGSLALAALESGIPSIRINPGN
                     MGPSHLRSIVASANEKKAVIRIGANGGSLSSSQLDAAGGVRSDALAAAVEEQLRILLA
                     EGFEDIILSAKSTSVMETVRANTLLASKYPDFPMHIGITESGYGRDGLVKSAAGLSLL
                     LAQGIGNTLRISLTEKPEEEVLAGYALLRALDIRSHGVTIVSCPTCG"
BASE COUNT          716 a          937 c         1041 g          989 t
ORIGIN      
        1 gttcaaggtc tccaagggga taggcttcct tacctcgggg cttattatct tttcagtatc
       61 agaggggatt cacccggtgt cgctcgttct cacactgagc gtatcggctt ttgtcgtgct
      121 ttttgtagaa ataatccgca ggcagcttcg aggggaaagt tttgcaattt ggaatatggg
      181 gggtacaatt tcgggtatag tatacatcac tattccgtgg accttcatga ttctgttgcg
      241 acgcctcccc ggaggagggc tcttattgct ggcgatgttc atcgctacat ggagctgtga
      301 tgttgcggcc tatcttatcg ggtcgcgctg ggggcgcact ctcctctgtg tcaacgtgag
      361 cccgaacaaa acgtgggaag gtttcttcgg gggcttttcg gcggcgctgc tcgcaagtgt
      421 gcttttggcg ttcatacttg agataccgcc gattccattt gtctacgtag ggataatctg
      481 cggttttgcg ggacagctcg gcgatctcgc agagtcggtt atcaagagag aaagctgggt
      541 aaaggacagc ggaaccctca ttccgggtca cgggggtgtg ctcgacagat ttgacagcac
      601 gctgatcaac ggtctgttgg tctatgtact tttcggggtg atcctgcgat gaagaaacgt
      661 ctttttgtca tcgggtgtac gggcagcgtc ggaagctctg tcctctccgt ttgccgtatg
      721 tttcccgaac acttcgaaat tgcggctctg gcagcgtgct cttctgtgga tcgtatgctt
      781 cttcttgcag aggaattttc ctgcccgacg cttgtctttt ccgatgaatc cgcggcagtt
      841 cgagctgcaa acgaactgct ctcttcgggc gttcaataca ccgggagaag agtccttgcc
      901 ggagaacagg gacttctgga gatggcatcg caccccgata ccgaccacgt ggtttttgcc
      961 tcttccggaa caggggcaat aggcgctctg atggcggcgc tcagtagcgg caaggaggtc
     1021 tccctggcaa acaaggagag cattgtcgtc gccggtccgt gggttctccc tctggttctt
     1081 cgccccgatc agatccgtcc gttggacagc gaacacaacg ctatttggca gtgcctttcc
     1141 ggggaagatc ggcgttttgt gaacaaaatt tttctcaccg catcaggcgg tcctttccgc
     1201 tctttttctc aagaggagat gaagcgggtg acgccttcga tggctgtcgc gcaccccgta
     1261 tggaacatgg gcgcaaagat aagcgttgac agcgcgaccc tgatgaacaa ggggattgag
     1321 atacttgagg cgatgtacct cttttctttt tccgctgacc aggtggacgc ggtcatctct
     1381 cccaacccct ttattcacgg gcttgtttgt ttttcagacg ggacttttca tatgtgcgcg
     1441 tccgccccgg acatgggcat ccccgcctta tcggctcttt tttcgcctct tcgcagtccg
     1501 cgcgacagaa agaacacccc tgttttggat ggaatgcaga tctcttttga ggcccccgac
     1561 gaggtcagat tcccatgtat tcgattggca cgcgaagttg cccggaagga aggcgcctac
     1621 ccggcgctcc tggtaggagc ggatgaggtt gctgtcgacc gcttcctttc aggccgcgtc
     1681 agcttcatga gtattcctcg tatcgttgaa tccgtgcttg aggagtattc cggaccgtcc
     1741 ccctcgacac ttgagcatgc tctgcatatt cttgaagaag gaaggcggat ggcggaaaag
     1801 gtgagttcac gattggaaga aaaataatgg tcaaggagat aagataaatg gtgagtttgg
     1861 tttcgttcct actggtaatc ggtatttgtg tcattatcca cgagcttgga cactatttta
     1921 cagcgcgcat attcgatgtt caggtgcatg agtttgcttt cggcatgggg ccagtgctcc
     1981 ttcagcggca aggccgccat accatgtggt cttttcgagc atttcccgtc ggggggttcg
     2041 tccgtcttgc aggaatgggg gaggaccacg aagaggaagt tgtcgaggag ggaagagggt
     2101 ttttcgacaa ggctgcctgg aagcgttttc tgattcttgc gaatgggtct gtgtccaaca
     2161 ttcttcttgc gatgcttctg acggttttct ttctctcggg ccacggtgtg atcaatcttg
     2221 agagcacaga aatcggcgag attatggagg ggtaccccgc gcaaacggcg ggtattcttc
     2281 ccggcgacgc cattgtcgct gtcaacgacg tacctgtttc ttcatggcgg gaaatgtctg
     2341 agagtattcg tacaacggcc tcagaaggac ctgttctttt ttcggtgctg cggaagggag
     2401 aaaccctctc tttgacagta accattccgc cggatgctca ggggattcct cttcttggaa
     2461 tacgaccggc gatgcgccgg tattccgttc ttgaggcttt tgcatccgca ttcacctaca
     2521 ctgtcgatat gacttttgaa atggtgcggg gaattatccg ctggataacc ggggttgaaa
     2581 aagtcgacgt caccgggccc gtaggtattg cctccatggc aggagacgct gcgcgcagag
     2641 ggatgtggcc ttttgtcaca tttcttgcct tgatcaatct gaacctgggg ctgctcaatc
     2701 tgttcccctt cccagccctt gacggaggcc gtctcatctt tacagcgggt gaaatgttgc
     2761 tcagaagacg ccttccggca cgaatagaaa attttattca tatgtcgggt tttgttttgt
     2821 tactcctgct tatcgtctat gtaacatggc aggatatcct tcgttttttc tagaggaagt
     2881 gaaagaatat gagctctcgt cagcttgctc tgcgtgatct cacaataggc ggcgaggcgc
     2941 cggttcgcgt tgagagcatg ctcaagattc ctctctctct tccggaggac tgtctcgcgc
     3001 agtgtttctc actgcgtaag gtcggatgcg agctggtgcg ggttgcattt ccatcggccg
     3061 atcttcgtgg caacttggag tggctgaatg atcggtcgcc gattcccctt atggccgata
     3121 tccatttcga tggatcgctt gctcttgcgg ctctcgaatc aggaattcct tcgatccgga
     3181 tcaatccggg aaacatgggg ccctctcacc ttcggagtat agtggcttcc gccaatgaga
     3241 aaaaagccgt gatccgtatc ggcgcgaacg gcggctctct ctcttcttct caactcgatg
     3301 ctgcgggcgg cgttcgatcc gacgctcttg ccgccgccgt agaagaacag ctccggattc
     3361 tgcttgccga agggttcgag gatattatcc tctcggcgaa atcgacctcc gtcatggaga
     3421 cggtgcgtgc gaacacactg ctcgcttcga agtatcctga ttttccgatg cacatcggca
     3481 tcaccgaatc gggctatgga agagacggac tcgtcaaaag cgctgccggg ctctcactgc
     3541 ttctcgcaca gggaatcggc aatacgctcc ggatcagtct taccgagaaa ccggaggaag
     3601 aggtgctggc cggatacgcc cttttgagag cgcttgacat tcgatctcat ggagtcacta
     3661 tcgtttcgtg ccctacatgc gga
//
LOCUS       DAOPKU010000294         3669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27979_length_3669_cov_4.808246, whole genome shotgun sequence.
ACCESSION   DAOPKU010000294 DAOPKU010000000
VERSION     DAOPKU010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27979_length_3669_cov_4.808246"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PK849_09470"
     CDS             complement(<1..918)
                     /locus_tag="PK849_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66392.1"
                     /translation="MRKGGVSPGVLLAGVVLFLITALYGGRGPEGRHPSDALPLPAEG
                     TLFLHYEGTKASGLSPLLKAMATVGGERAPTLDALFFVPPLFLNAGETAALLAWEENA
                     LHFYMAARLPKEHTQAISEGVFPKGIAPTLTGVSLQEVSPGIFGVRRKNEDLSLFISV
                     NGGITLFASSPEKMSRMALTLERQAEPIDIAWKLERRWPNHIFFSDGGLFSGIASMEG
                     LPAAKENLLVTAAWRDEDDGGRMEWHLEGLDTIFPPSLLKEFREAHWGGRYTFPEPLV
                     AAVGFTIPERFAAFLEDGRMSEAFGLWENA"
     gene            complement(970..2574)
                     /locus_tag="PK849_09475"
     CDS             complement(970..2574)
                     /locus_tag="PK849_09475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE66393.1"
                     /translation="MRLKIIGAAGEVTGSSYLLECGASRVLVDCGIFQGKDDERKNRE
                     LFPFSSVSLNAVLLTHAHMDHSGRIPLLVKEGFKGKVWATLPTLELVKVLWYDSAHLM
                     KEEADWRTKKNSRKGLDPVEPIYTRDDVDDAIKMLSAASYDDKIPVAPGISVRFRDAG
                     HILGSAILEVWLEEEGKQVKVVFSGDLGPQQTVMERNPAIITSADYVIMESTYGDRLH
                     KTNAESRDEFRDVLITALKDNAKVFIPSFVVDRAQRILYELALLKDEGILAGNVPIFF
                     DSPMGVKATEIYKKHMNLLSSEIQQYVSQGKDPFAPGKLKYVSSVEESQAINDVGNAV
                     VVAGSGMANGGRIVHHLKHGIWNPKNHVVFVGYQAQGTLGRRIVEGEKNLRIAGEDVS
                     VKANIHTINGFSAHADRDDLLAWAGNFSTAPLFLITHGEPKSSLAFSETLAAAGMKGV
                     VPVAGQEIELEPNGAGKAVHIAEQPVVLKREEVSTLLAEILLLASGLQNRAVPVQEEG
                     ILSLLRSSKVLLETANEKMNERLRAE"
     gene            complement(2543..>3669)
                     /locus_tag="PK849_09480"
     CDS             complement(2543..>3669)
                     /locus_tag="PK849_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPE66394.1"
                     /translation="RPNIPIGRAVEIKDKDGDLYTLAVYPVRGGERFVIAAVAWEQLL
                     GPLARGGMLWSVLIVIMAICILLALWALWRWLIRPLRSLVAELEGLSWGRDLPGASDP
                     RAVGEIGSLREVLYKLASAAVERTHLRNRYVNDIVRVQEGERSRIARELHDGPLQNIT
                     AMIQQVRLSRMSRDEDVVKERLLLAEETAHLVVRELREMCDELSPPWIDLGLEQAMTE
                     LSERLARHYNINIVMEIDDSLELDHEKLLSLFRIFQEAVSNAVRHGKAKEMRVNIFRN
                     ADNTIFEMKDDGTGFDTRFNYESLRVEGHRGLANMLERMTLVGGKLEVISQKGDGALI
                     RCILPIAPSEEADCRNSVPKLEGGIPQGNHHETKNYWSRR"
BASE COUNT          857 a         1050 c          878 g          884 t
ORIGIN      
        1 cgcattttcc cacagaccga aagcctcgct catgcggccg tcctcgagga aggccgcaaa
       61 cctctcgggg atcgtaaatc cgactgcagc gacgagaggc tcggggaaag tataacgccc
      121 tccccagtga gcttcccgaa actctttcag caaggaaggg gggaaaatag tatcaagccc
      181 ttccaaatgc cattccatgc gtccgccgtc atcttcgtct ctccatgccg ctgttacgag
      241 gaggttttct ttagctgcag gcagcccctc catcgaggca atcccggaaa acaaccctcc
      301 atcggaaaag aaaatgtgat taggccaccg tcgttccaat ttccaggcaa tatctattgg
      361 ttctgcctgt cgttccagtg tcagcgccat tctactcatt ttttcgggag atgaggcaaa
      421 cagcgtgata cctccgttta cgctgataaa aagagacagg tcctcatttt tcctgcgtac
      481 accaaagatg cccggagaga cctcctgcaa cgacactcca gtaagagtcg gcgcaatacc
      541 ttttgggaac actccttcag agatggcctg cgtatgctct ttcggcaatc ttgctgccat
      601 gtaaaagtgc agagcgtttt cttcccaggc gagcaaagcg gcagtttcac cggcgtttaa
      661 aaagagcggc gggacaaaaa ataaagcgtc caatgtggga gcacgctccc ctccgactgt
      721 cgccattgct ttaagcaacg gcgacagtcc tgaagccttt gtaccttcat agtgaaggaa
      781 gagcgtgcct tcagcaggca gcggaagagc gtcagagggg tgacgccctt cagggccccg
      841 tcccccgtac agggccgtaa tcaaaaacag aacgacaccg gcgagaagaa ctccggggga
      901 gactccacct ttacgcatac ctccacctcc gtgcccagat cgatacggga aaatacgacc
      961 tgcgacagtc tattccgccc gtaaccgctc gttcattttt tcgttcgccg tttcgagaag
     1021 caccttgctt gacctcagaa gcgagagaat tccctcttcc tgaacaggga cggcacgatt
     1081 ttgaagcccg gatgcaagga gaagaatttc ggcgagcagc gttgaaacct cttctctttt
     1141 aaggaccacc ggctgttcag ctatgtggac cgcttttccc gccccgttag gttcgagttc
     1201 tatctcctgc ccggcaacag gaacaacgcc cttcatgcct gccgctgcaa gggtttctga
     1261 aaacgcaaga gaagatttcg gttcgccgtg agtaatcagg aagaggggcg ccgtcgaaaa
     1321 attacctgcc catgcaagca gatcgtcacg atccgcatgc gcagaaaaac cgtttatcgt
     1381 atgaatattc gcttttactg agacatcttc tccagcaatc cgtagattct tctctccttc
     1441 tacaattctg cggccaaggg tcccctgagc ctggtagccg acgaaaacaa catgattctt
     1501 tggattccag atcccgtgct tcagatggtg gacgattcgc cctccgtttg ccatcccgct
     1561 tccggcgacc accacggcat ttccgacatc attgatcgcc tgagactctt caacgctcga
     1621 tacgtacttc agtttacccg gagcaaacgg gtctttccct tgacttacgt actgctggat
     1681 ttccgacgaa agaagattca tgtgtttctt gtagatttcc gtggctttga cgcccatcgg
     1741 agagtcaaag aaaatgggga cattgccggc cagaattcct tcgtccttta acagagccag
     1801 ttcgtagagg attctctgcg cccggtccac aacgaagctc gggataaaaa cctttgcgtt
     1861 gtctttgaga gctgtaataa gaacgtcgcg gaattcgtcc ctactctcgg cgttggtctt
     1921 gtgcaagcga tctccgtacg tggattccat gatcacgtag tcggccgatg taataatagc
     1981 cgggtttctt tccatgacag tttgctgggg cccaagatcg ccgctgaaga caaccttgac
     2041 ctgtttcccc tcttcctcca gccagacttc gagaatggcg cttccaagga tatggccggc
     2101 atctctgaat ctcaccgata ttccgggggc aacagggatc ttgtcatcgt aacttgcggc
     2161 tgagagcatc ttgattgcgt cgtcaacgtc gtcacgggtg tatataggct caacagggtc
     2221 gagtcctttt ctggagtttt tcttcgtccg ccagtccgcc tcttccttca taaggtgtgc
     2281 ggagtcgtac caaaggactt tcacgagctc cagtgtaggc agcgtagccc atacttttcc
     2341 tttaaacccc tccttgacga gaaggggaat tcttcccgaa tggtccatgt gcgcgtgagt
     2401 taaaaggacc gcgttcaaag aaacagatga gaaagggaac agctcgcggt tttttctttc
     2461 gtcgtctttt ccctgaaaaa taccgcaatc aacgagtacg cgacttgctc cgcactcaag
     2521 aagatagctt gatccggtaa cttcaccggc ggctccaata atttttagtc tcatggtgat
     2581 tcccttgcgg gatccctcct tccagtttgg gaacagaatt tctgcaatca gcctcttcag
     2641 agggagcgat tggaagaata cagcgaataa gggccccatc gcccttttgt gagatgactt
     2701 ccagctttcc tccgacgagc gtcatacgtt caagcatgtt tgccagtccc cggtgcccct
     2761 ccaccctgag actttcataa ttaaaacgcg tatcaaaacc ggtaccgtcg tctttcatct
     2821 cgaagatggt attgtccgcg tttctgaaga tgtttacacg catctctttt gccttcccgt
     2881 gccgaactgc attcgatacg gcttcctgaa agatacggaa caacgaaagg agtttttcat
     2941 ggtccaactc caacgaatcg tctatttcca tcacgatatt gatattataa tgacgtgcca
     3001 gccgttcgct cagctccgtc atagcctgtt caagccccag atcaatccac gggggcgaga
     3061 gttcgtcgca catctcccgc agttcccgaa caacaagatg cgcggtttcc tctgcgagca
     3121 gaaggcgttc ttttacaaca tcctcatccc ggctcattct tgaaagacga acctgctgta
     3181 tcatggcggt aatattctgc agaggtccgt catgcaattc tcgcgctatc ctggaccttt
     3241 ccccctcctg gacgcggaca atatcattta cgtatcggtt gcggaggtgc gttcgctcta
     3301 cagccgcgct tgcaagcttg taaaggacct cccggaggct gccaatctct ccaaccgctc
     3361 tcgggtctga cgcgccgggg aggtctcgtc cccaactaag cccttcgagt tcggcgacaa
     3421 gacttcggag cgggcggata agccatcgcc acaatgccca aagcgccagc agaatacata
     3481 tcgccataat gacaataaga acagaccaga gcataccccc tcgtgcaagg ggaccaagga
     3541 gctgttccca ggcaacagcg gcaataacaa accgttctcc tccccggaca gggtacacgg
     3601 caagagtata caaatccccg tctttatcct tgatctcaac ggctcttccg atcgggatgt
     3661 tcggacgcc
//
LOCUS       DAOPKU010000295         3669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_27980_length_3669_cov_4.114831, whole genome shotgun sequence.
ACCESSION   DAOPKU010000295 DAOPKU010000000
VERSION     DAOPKU010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27980_length_3669_cov_4.114831"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..511
                     /locus_tag="PK849_09485"
     CDS             86..511
                     /locus_tag="PK849_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPE66395.1"
                     /translation="MKYAATDEIIAIRLSEGEDLADSLVHVCKACDVDSAVIVSAAGM
                     VSSVTFGWYNGRDYNTESVREIMELTSLSGNVSFRGGSLYPHLHGVFNKPDHAALSGH
                     LLQAIVFNNAELFLKPLATVHLGRSFDGSFEALSPEKKM"
     gene            complement(571..1026)
                     /locus_tag="PK849_09490"
     CDS             complement(571..1026)
                     /locus_tag="PK849_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPE66396.1"
                     /translation="MEKVLRFLNERGFHGAVTYTEETIFTVEDASRVVGAQPEHILKS
                     LVLLVDDRPALALMSGTNRVDVKKVRREAEAKKVRMADPEYVYEWSGFKIGGVPPVGY
                     PELPEAFLDEDLFLYPVVWAAAGTDHSFFPIAPGDLLRLAEGRKCSIKK"
     gene            complement(1096..1977)
                     /locus_tag="PK849_09495"
     CDS             complement(1096..1977)
                     /locus_tag="PK849_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPE66397.1"
                     /translation="MDFRELQSLVEVMEKGSISAAAASLGISQPAVSKHIAKLEREMG
                     VKVFARGQKCSNLTVEGEVLYKFARKTISQLADIKRDFSDIADDVSGIVRISASSIPG
                     DYILPGILVEFRKLYPNIEVEVRISDSREAVEKLVTRESDISVTGHGRHASGFNTVPF
                     ARDELVLLVAPGHPLAERENITLRDLENMDLVGRVTGSSTSRIWEDAFKAHTGKGKHV
                     YLKFGHVNAVVEAVRNGAEGAVISRLAGEDVSGLVMIPFRPALMRTFSITYGTMTTKA
                     METLLEFLLERKTFKDE"
     gene            2168..2539
                     /locus_tag="PK849_09500"
     CDS             2168..2539
                     /locus_tag="PK849_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPE66398.1"
                     /translation="MSLGLRIRTLRKALGLTQQGLAERTQVSRIYIQALESNRRTPSM
                     KLLSRLANSLEVEVQDLVKTMSTSPSGRLQLEEVFQAAPETEIWYRSKRLKPKELRFV
                     QSLIDAAIERWEEEDRADGSV"
     gene            2566..2997
                     /locus_tag="PK849_09505"
     CDS             2566..2997
                     /locus_tag="PK849_09505"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HPE66399.1"
                     /translation="MGSRKQSQSTEFTFPSPPDFLPEWALREGGDWAGLDEFSLLVKA
                     EERTGKILELEYHDLPFGTWGVHIVRGRRGLIFINKRLSPYWRRFALFHELYHFLEHN
                     GGMELWTRTATPLSSFEHQADLFAWGALRKEWGRCWAHAFP"
     gene            3033..>3669
                     /locus_tag="PK849_09510"
     CDS             3033..>3669
                     /locus_tag="PK849_09510"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE66400.1"
                     /translation="MDSCPRRCVYCHQGEITGISEVPSPDAVQQTLSRLQAPHEVCYF
                     GGSFTCFPEERRRAYMEAVFAAPERSCVRFSTHPHCLSPGILDSLASYPVSMIELGIS
                     SLDDDVLNACNRGYTGEFAIAAMRLILERGFSLGAQLMIGLPGQTEESSLNDLRRIAA
                     LRGQREPFPVTLRIYPCLVLDKTPLAELMRKGAYAPLSLEEGILRAGRLHFE"
BASE COUNT          903 a          977 c          901 g          888 t
ORIGIN      
        1 tacatggtag aatattgaag gaataaaaga gaactgcttt ccgccgggca atgcacgccg
       61 gaaatatgaa aggaggacga gaaacatgaa atatgccgct accgatgaaa ttatcgcgat
      121 acgtctttcg gaaggagaag atctcgcaga ttctctggtg catgtctgca aggcatgcga
      181 cgtcgattcg gcagttatcg tctccgcagc gggaatggtg tcttccgtaa ctttcgggtg
      241 gtacaacgga cgtgattata atacggaatc agtgcgtgaa ataatggaac ttacctcgct
      301 cagcggcaat gtaagcttcc gcggaggcag tctctacccg catcttcacg gcgttttcaa
      361 caaacctgat catgccgcgc tttccggaca tcttctccag gccatcgtat tcaacaacgc
      421 ggaacttttt ttaaaaccgc ttgcaacggt tcatcttgga cgctctttcg acggctcttt
      481 tgaagccctt tcccctgaga aaaagatgta agatcgcaca cagtgcgacc caaaaagttc
      541 ctcccgaaaa cgggaggaac tttttttcgc ttactttttg atgctgcact tcctgccctc
      601 tgcgaggcgc aacaaatcac cgggagctat gggaaaaaac gaatgatccg ttcctgcggc
      661 agcccagaca acggggtaca aaaagagatc ttcatcaaga aacgcctcag gcaactccgg
      721 atatcccaca ggaggcacgc ctcctatttt gaaaccggac cactcgtaaa catactccgg
      781 atctgccatt cgaacttttt tcgcttccgc ttctctgcgt actttcttaa catccacgcg
      841 gttcgttccg gacataagtg caagcgccgg ccgatcgtct acaagaagca ccaagctttt
      901 caaaatatgc tccggttggg cgccgacgac cctggatgcg tcttccacag taaagattgt
      961 ttcttccgtg tatgtgaccg ctccatgaaa acctctctca ttcaaaaagc ggaggacctt
     1021 ttccaccggg tcggaagacg actcttcgat aacgaaggtc tcctcttgtg catgctgaat
     1081 tacagagtcc actttctact cgtccttgaa ggtttttctc tcaagcaaaa actcgagaag
     1141 cgtctccatc gcctttgtcg tcatggttcc gtacgtgatc gaaaatgtcc gcataagggc
     1201 gggacggaac ggtatcatta caagcccgga gacgtcctcg cccgcaagac gggaaatgac
     1261 tgctccctcg gccccgtttc gtacagcttc aacaaccgca ttgacgtgac cgaatttgag
     1321 atagacatgc ttcccttttc cggtatgggc tttgaatgca tcctcccaga tacgcgatgt
     1381 cgacgaaccg gtgactcttc ccacaaggtc catgttctcc agatcgcgca gagtgatgtt
     1441 ttcgcgctcc gcaagcgggt gtccgggagc gactagaaga accagttcat ctctggcgaa
     1501 aggaaccgta ttgaatccgg atgcatgtct cccgtgtccc gtaacggaga tatcggactc
     1561 ccgggtaaca agtttctcaa cggcttctcg cgaatctgaa atgcgcacct ccacttcgat
     1621 atttggatag agtttccgga actctacaag aataccggga agaatataat cgcccggtat
     1681 ggagcttgcg cttatcctca caatacccga tacatcatcg gctatgtccg aaaagtcgcg
     1741 tttgatatcc gcaagctgag agatagtttt acgggcaaat ttatacaaca cttccccttc
     1801 tactgtcagg ttggaacatt tctgtccccg ggcaaatact ttgaccccca tctctctttc
     1861 aagcttcgcg atgtgtttgc tgactgccgg ctgagaaatt ccgagtgatg ctgccgctgc
     1921 cgagatgctc cctttttcca taacttctac cagactttgc agttcacgaa aatccaaagc
     1981 ggatacctcc ccaaagacaa tatattcttt ttccatccaa acgccccgac ataaatatat
     2041 tctgaaagag aaagaacttc aagagccaaa gatatttttc ttcaaaagaa ggcaagggct
     2101 tgaacttaat gtaatcaagt atataataat atatatccaa agaaaacaag acggaggagg
     2161 gatcgcgatg agccttggac tgaggatacg cactcttcgt aaggccttgg gcctgacaca
     2221 gcaggggctt gcggagagaa cacaggtgag caggatatac atacaggcat tggaaagcaa
     2281 ccggagaact ccttcgatga aactcctctc gcgccttgcg aattcacttg aggtggaagt
     2341 acaggatctg gtaaagacaa tgtcgacatc cccttcaggg agattgcaac ttgaagaagt
     2401 ctttcaagcc gctcccgaga ccgagatctg gtacagaagc aagagactga aacccaagga
     2461 actgcgtttc gtccagagcc tcatcgacgc ggcaatcgaa cgttgggaag aagaggaccg
     2521 cgccgatgga agcgtctgat ccttcttcac gccggatttt ctgttatggg cagtcggaaa
     2581 cagtcccaaa gcactgaatt cacgttccct tcgccccctg actttttacc cgagtgggcg
     2641 ttgcgagaag gcggcgattg ggccggtctc gatgagttct cgctcctcgt aaaagccgaa
     2701 gaacggacgg gaaagattct tgagctcgaa taccacgatc tgccatttgg tacatggggc
     2761 gtacacatag ttcgtggacg aagggggcta attttcatca ataaacggtt gtcgccttac
     2821 tggaggcgct tcgctctttt tcatgaattg taccattttc tggagcacaa cgggggaatg
     2881 gagctgtgga cccgtaccgc tacgccgctc tcgagtttcg agcatcaagc ggatctcttc
     2941 gcatggggcg cgctgcgcaa ggaatggggc agatgctggg ctcacgcatt tccatgagcg
     3001 ccgctctccc gaaaattcca ttttttcttc caatggattc ctgcccgagg cgttgtgtct
     3061 actgccacca gggagagatc acaggaatct ccgaagttcc ttctccggac gcagtacagc
     3121 aaaccctctc ccggcttcaa gctccccatg aggtttgtta tttcggaggt agctttacct
     3181 gtttcccgga agagcgcagg cgcgcgtaca tggaagccgt cttcgccgct ccggagagga
     3241 gctgcgttcg tttctcaaca catccgcact gtctctcgcc cggtattctc gacagtcttg
     3301 cttcctaccc cgtttcaatg atcgaactcg ggatctcctc gctcgacgac gatgtgctga
     3361 acgcatgtaa ccgggggtac acaggagagt tcgcgattgc agcgatgcgg cttattttgg
     3421 aaagagggtt ttctctcggg gcgcagttga tgatcggtct tccggggcaa acagaggaaa
     3481 gttctctgaa cgatctccga cgaattgccg cgctccgcgg gcaaagagaa ccctttccag
     3541 taacgcttag aatctatcct tgtcttgttt tagacaagac gcccctcgcc gaactcatga
     3601 gaaagggcgc ctacgctccg ctctcgcttg aagaaggaat tcttcgcgcg ggcagattgc
     3661 acttcgagg
//
LOCUS       DAOPKU010000296         3663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_28043_length_3663_cov_4.701774, whole genome shotgun sequence.
ACCESSION   DAOPKU010000296 DAOPKU010000000
VERSION     DAOPKU010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28043_length_3663_cov_4.701774"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /gene="ftsH"
                     /locus_tag="PK849_09515"
     CDS             complement(<1..1570)
                     /gene="ftsH"
                     /locus_tag="PK849_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPE66401.1"
                     /translation="MGRLVKNLGLYLILIVLVVSLVNVFLSPTQGPQQVQEIGYSTFL
                     SEVTAGRVSNVLVRENSIKGRFSDGKEFVSYVVGIGDLAKEVAQKGVAVEVEPPQRTP
                     WWANMLSSLFPTLLLIGVWIFFLYNMQGGGGKVMNFAKSKAKLFIDNRPKVTFKDVAG
                     CDESKEELSEVVYYLRDPSRFTALGARVPRGVLLLGPPGTGKTLLARACAGEADVPFF
                     SVSGSDFVEMFVGVGAARVRDLFEQARKYQPCIIFIDEMDAVGRHRGAGLGGGHDERE
                     QTLNQLLVELDGFDETTGIILIAATNRPDILDPALLRPGRFDRHIVVDRPDVKGREAI
                     LDVHVREKKLANDVDLAVLARRTPGFVGADLANLVNEAALLAARTGEKEIKMKDFEEG
                     IDRVIAGPERKSRLVSDKEKLIIAYHETGHALVAKRIPSCDPVHKISIIPRGHMALGY
                     TLQLPEEDRFLMSKNELLDKICVLFGGRVAEELRFGDVTTGASNDLERATQIARQMVT
                     EFGMSEKLGLVKLGH"
     gene            complement(1611..2144)
                     /gene="hpt"
                     /locus_tag="PK849_09520"
     CDS             complement(1611..2144)
                     /gene="hpt"
                     /locus_tag="PK849_09520"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPE66402.1"
                     /translation="MEYQISAILLSQEELAARVREIGETIGRDYAGKELVVIGILKGA
                     VIFLSDLVRAVPADVNITLDFMAVSSYGDSTKTSGIVRIVKDLDGSIKDKDVLLVEDI
                     VDTGLTLSYLVKMMRERQPKSVRVCALLDKEERRKVHVDVDYRGFPIPDEFVVGYGLD
                     YGGRWRHLPAIYTIEQK"
     gene            complement(2155..>3663)
                     /gene="tilS"
                     /locus_tag="PK849_09525"
     CDS             complement(2155..>3663)
                     /gene="tilS"
                     /locus_tag="PK849_09525"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPE66403.1"
                     /translation="EHAVHTRSVTGSIPVTATTKDKSKRRRKSPPFFPFQEVAVTSKE
                     KVSQLKTIPYSHSQERLRTIFHDIGIRQGWWRTDRPMLLAVSGGSDSMALMWFFHTLW
                     EGHCIVAHLNHGIRGESADKDQRFVEEIATRFSFPVESASYPIPELLERGESLEDGAR
                     RIRYEFLEKTMKQTGACGVAVAHTSDDSAETFLMNLMRGTGVRGLVGVPELRDSFIRP
                     LLAFSKNYLKEILEFHGIPWREDLSNRDNAYTRNRVRNSLIPFLQKEFNPRVKEGILG
                     IAEDMKHFRENEEEMQSALVDLLERRVPFTEYACSLQAMRSLDEQTCAVFVRGVGARL
                     GLRTLSRDRTNTLCRLVHKSSRWCFQWQKEIVLFCSFHLVAWIDPAILVQSAGASPSS
                     FALEDQSGFFEWGKWLFSWKREWASASNYGTMGAVFPNSERIEVVPFALFRKAIRKRD
                     IPEWAVSLFPVVYSDRMQWLPYWQGSGRWESLHDVAIRISAKYLQNIDERKR"
BASE COUNT          885 a          979 c          902 g          897 t
ORIGIN      
        1 tatgtcccag ttttacaagt ccgagcttct cgctcatgcc gaattcggta accatttgtc
       61 gggcaatctg tgtcgcccgt tcgaggtcgt tgctcgcacc ggtagttacg tccccgaacc
      121 tgagttcctc ggcaactctg ccgccgaaaa gaacgcaaat tttgtcgagg agttcgtttt
      181 tcgacatgag gaagcgatcc tcctcgggga gctggagcgt gtaccccaat gccatgtgtc
      241 ctcgggggat tatcgatatt ttatgaaccg gatcgcatga aggaattcgt ttcgcaacaa
      301 gcgcgtgccc cgtttcatgg tatgcgatta ttagtttctc tttatcgctg acgaggcgac
      361 tcttcctttc cgggccggct atgacacggt cgatgccctc ttcgaaatct ttcattttga
      421 tctctttttc acccgtgcgt gcggctaaaa gcgcagcttc gtttacaagg ttggcaagat
      481 cggcgccgac aaaacccggt gttcggcgcg cgaggacggc aaggtcgacg tcgttcgcaa
      541 gcttcttttc gcggacgtga acgtcaagaa tagcctctcg tcctttaacg tcgggtctgt
      601 cgacaacgat atgtctgtcg aaacgaccgg ggcgcaacaa cgccgggtcg aggatatcgg
      661 gccggttggt agctgcgatc aggattatcc ccgtagtctc gtcaaacccg tcaagttcaa
      721 ccagaagctg gttgagcgtc tgctcgcgtt cgtcgtgtcc tccgccgagt ccagcgcctc
      781 tgtgacggcc aactgcatcc atttcgtcga taaaaatgat acagggttga tatttcctgg
      841 cttgctcgaa aagatcccga acacgggcgg ctcctacgcc gacgaacatc tcgacgaagt
      901 ccgatccgct cacgctgaaa aaaggaacat ccgcttcgcc ggcgcaagca cgggcgagca
      961 gcgttttccc ggtaccagga ggtccaagca gcagaactcc gcgagggact ctggcgccga
     1021 gggcggtgaa acgggaagga tcccgaagat agtagacgac ttctgaaagc tcttctttcg
     1081 actcgtcgca cccggcgacg tctttaaatg tgactttcgg gcggttgtca atgaagagct
     1141 tcgccttgct tttggcgaag ttcatcacct tgccgccccc gccctgcatg ttgtaaagaa
     1201 agaaaatcca cacgccaatc agcagcaggg tcgggaagag tgaagagagc atatttgccc
     1261 accaaggagt tcgttgcggg ggttcaacct ccacggcgac gcccttttga gcgacttctt
     1321 ttgccaaatc gcctattccg accacgtatg agacaaactc tttaccgtcg gaaaatctgc
     1381 ctttgatgga gttttcgcgt accaggacat ttgaaactct cccggctgta acttcggaga
     1441 gaaacgtgct gtaccctatc tcctgaactt gctgaggccc ctgtgtcggc gagagaaata
     1501 cgttgaccag actcacgaca agtacaatga gtatgaggta taaaccaaga ttttttacca
     1561 atcggcccaa attatagccg cctcctgact aaaaatagta ttgtaaatag ttacttctgt
     1621 tcgattgtat aaatagcggg cagatgacgc cagcgtccgc cataatcaag cccataccct
     1681 acgacgaact cgtccggtat gggaaagcct ctgtaatcga catccacatg taccttacgt
     1741 cgttcctctt tatcgagcag tgcgcacaca cgaacgctct tcggttggcg ttcccgcatc
     1801 atcttcacaa gatatgacag ggtgagtccg gtgtcaacaa tatcctctac cagcagtaca
     1861 tctttgtctt tgatgctgcc atccagatcc tttacaatac gaacaatacc gctggtcttc
     1921 gtggaatccc cataggaaga gacagccatg aaatccaaag ttatgttgac gtcggcggga
     1981 actgccctca ccaagtcgga aagaaaaata acagctccct tgaggatgcc tatgactacc
     2041 aattctttac cggcataatc gcgtccgatc gtctctccta tctcccggac gcgggcagcc
     2101 aattcttcct gtgaaaggag aatcgcagaa atttgatact ccattgtcgt tacgtcacct
     2161 tttcctttca tcaatgttct gcaggtattt tgcagaaata cgaatcgcaa cgtcgtgcag
     2221 actttcccag cgaccgcttc cctgccagta gggaagccac tgcattcgat cagaatacac
     2281 caccggaaag agagacacgg cccattcggg aatatctctc ttccggattg cttttctgaa
     2341 cagcgcaaaa gggacaacct caatcctttc gctgtttgga aatacggctc ccattgtacc
     2401 gtaattactt gcggatgccc attccctctt ccaagaaaaa agccactttc cccattcaaa
     2461 gaaaccggat tggtcttcaa gagcaaaaga ggatggggag gcgccggcag actgaactag
     2521 tatagcaggg tcaatccatg cgacaaggtg aaaactgcag aaaagaacga tctctttttg
     2581 ccactggaaa caccaccgcg aagatttatg aacaagtcgg cagagggtgt ttgtcctgtc
     2641 acgggaaagc gttcgaagtc cgagcctggc tccaacccct ctgacgaaca ccgcacacgt
     2701 ctgctcgtcg agcgagcgca ttgcctgtaa agaacaggcg tactcagtaa agggaacgcg
     2761 tctttccagg agatcgacga gtgcggattg catttcttct tcgttctccc ggaaatgttt
     2821 catgtcctct gctataccaa ggattccttc ttttaccctt gggttgaatt ctttttgtag
     2881 aaatggaatc agagaattac gtacccggtt tcgggtgtat gcattgtctc tatttgaaag
     2941 atcctcccgc cacgggattc cgtgaaactc gagaatttcc ttgagataat tcttcgaaaa
     3001 ggcgaggaga ggtctgataa aggagtctct tagttcaggg acgccgacca atcctcggac
     3061 tcctgttccc ctcatcagat tcattaaaaa cgtctccgcg gaatcatctg aagtatgcgc
     3121 gacggctact ccacaagcgc ctgtttgctt cattgtcttt tcgaggaatt cgtaacgaat
     3181 acgccgtgcg ccgtcttcaa gcgattcccc ccgttcaagg agttcaggta tggggtacga
     3241 agcgctttcc acgggaaatg aaaaacgggt agcaatctcc tcgacgaagc gctgatcctt
     3301 gtccgcgctt tcacctctga taccgtgatt gagatgcgca acgatacaat gtccttccca
     3361 cagggtatgg aaaaaccaca tgagcgccat ggagtcgctc ccgccggaga cggcgagcaa
     3421 catcgggcgg tcagtgcgcc accacccctg gcggatgccg atatcatgga aaattgtgcg
     3481 taaacgctcc tgagaatgag aataagggat ggtttttaat tgagaaacct tctcttttga
     3541 agtcacggcg acctcctgaa aaggaaaaaa aggcggagat tttctccgcc ttttactttt
     3601 gtcttttgtg gtggcggtga ctggaatcga accagtgaca ctgcgggtat gaaccgcatg
     3661 ctc
//
LOCUS       DAOPKU010000297         3662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_28053_length_3662_cov_4.267258, whole genome shotgun sequence.
ACCESSION   DAOPKU010000297 DAOPKU010000000
VERSION     DAOPKU010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28053_length_3662_cov_4.267258"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PK849_09530"
     CDS             complement(<1..668)
                     /locus_tag="PK849_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66404.1"
                     /translation="MKIAVASGKGGAGKTSIAASLVLAKRGVLAMDLDVEEPNLGILL
                     GCSPGESCPVHQPVAYVEPDLCSGCGRCAQACAYGAIAWFGSGLPVVNESLCHGCGVC
                     PLVCPTGAMREREKVIGEIRSGFVDGAPFLEGRLEVGGVSTVRLIEATIQAGESLHAD
                     DWIMDAPPGASCPVSAVLREADAAILVVEPTPFGLSDVAGVLEVAADMKKPAGIVANK
                     TGMG"
     gene            complement(665..1027)
                     /locus_tag="PK849_09535"
     CDS             complement(665..1027)
                     /locus_tag="PK849_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPE66405.1"
                     /translation="MTKAAIAMDSGVISEHFGKTKEFLFITFEGKNEISREILPPPAE
                     EHVPGLFPNWVKKMGADVVVAGGMGTQAKRMFESLGVGVCTVPSMGVEQAVEALLSGS
                     LQGVDMECTHGHDHSCGK"
     gene            complement(1024..1308)
                     /locus_tag="PK849_09540"
     CDS             complement(1024..1308)
                     /locus_tag="PK849_09540"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPE66406.1"
                     /translation="MPGRNGTGPMGQGSMTGWGRGPCGCGVAAGGRFFRGRGCFGWGM
                     RMGMGMGRGGVWAEPYTGADEQTLLEQELAMTEARAQELRARLGKGGDAE"
     gene            1481..2032
                     /locus_tag="PK849_09545"
     CDS             1481..2032
                     /locus_tag="PK849_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPE66407.1"
                     /translation="MTNNTRTDETVQAFLEKLKDRGLRLTAQRETLFRVIAEDLGNPT
                     SVQNIWDRTREVDPSIGIATIYRTLNLLGEMGVVNIIYLSDGEFRLDVPLQKIHLTAF
                     CRHCGALFPLPAEDEKQHTLEKWLEGTGLELLPQSVAVAGLCTECREALKSPDALPAR
                     RGPLGQQGPCLRRRCRRMGRVQE"
     regulatory      2224..2331
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2426..3466
                     /locus_tag="PK849_09550"
     CDS             2426..3466
                     /locus_tag="PK849_09550"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HPE66408.1"
                     /translation="MVCRALSHQNHPLKIRRVRSPDTDPWKNLAREEFLMGDCADDEA
                     IFYLWQNAHTVVIGRNQNAWSECRLDLLESEGGRLARRSSGGGAVYHDLGNLNYSFIM
                     PRPLYSMERQLGVVLAALRSLGINAEFSGRNDLLLEGRKFSGNAYQLTRRRGLHHGTL
                     LLSVDMSKLSRYLNVDPEKLQSKGVTSVASRVVNLAEASPDLTRDALMDALERAFLEE
                     YPHNEVRRESDEPESEAFTRLYEKYSGREWRYGRVPECAAAIRKRFGWGGVELRCDAP
                     AGRIRGAQVFSDAMDGEMILSLAKALEGLPFSWKEMAEAVRAAFPGSPEMADVAEWLE
                     EAPIGRHKEENA"
     gene            3463..>3662
                     /locus_tag="PK849_09555"
     CDS             3463..>3662
                     /locus_tag="PK849_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPE66409.1"
                     /translation="MKRTPMYEKHVAAGGRMVDFGGWELPVQYEATGIKTEHLTVRSK
                     AGLFDVSHMGEITVEGRGAQAWL"
BASE COUNT          730 a         1212 c         1089 g          631 t
ORIGIN      
        1 tcgcccatgc cggtcttgtt cgcgacaatc cccgcgggct ttttcatgtc cgcggcaact
       61 tcgagcacac ccgcgacatc cgaaaggccg aacggcgtcg gctcgacgac aaggatggcg
      121 gcgtccgcct cgcgcagcac cgccgacacg ggacaggaag cgcccggagg cgcatccatg
      181 atccagtcgt ccgcgtgcag cgactcgccc gcctggatcg tcgcctcgat aagccgtacc
      241 gtgctcacgc ctccgacctc aagccgtcct tcgaggaagg gcgccccgtc gacgaatccg
      301 gagcggatct cgccgatcac cttctcgcgc tcacgcatcg cccccgtcgg gcagacgagc
      361 ggacagacgc cgcacccgtg acagagcgat tcgttcacca cgggaaggcc ggagccgaac
      421 catgcgatgg cgccgtacgc gcacgcctgt gcgcatctgc cgcagcccga gcagagatcg
      481 ggctccacat acgccacagg ttgatgcacc gggcacgatt caccgggcga acaccccaga
      541 agaatcccca gattgggttc ctccacgtcg agatccatcg cgagcacccc tcgctttgca
      601 agaacaaggg aggccgcgat gctggtcttg cccgcgcccc ccttgccgct cgccacggcg
      661 atcttcattt tccgcaggag tggtcgtgtc cgtgggtgca ctccatatcg acgccctgca
      721 gagaaccgga gaggagcgcc tccacagcct gctccacacc catggaggga accgtgcaga
      781 caccgacgcc cagcgactcg aacatccgct tcgcctgcgt tcccatgcct cccgcgacca
      841 ccacgtcggc ccccatcttc ttcacccagt tcgggaagag cccgggaaca tgctcttcgg
      901 cgggaggagg aaggatctcg cgcgagatct cgttcttccc ctcgaaggtg atgaaaagaa
      961 actccttcgt cttgccgaag tgctcggaaa taacgccgct gtccattgca atcgccgctt
     1021 tcgtcactct gcgtccccac cctttcccag tctggcgcgc agctcctgcg cccttgcctc
     1081 ggtcattgca agctcctgtt cgagcagtgt ctgttcgtcg gccccggtat acggctcggc
     1141 ccacacgccg cctctgccca tacccattcc catacgcatg ccccaaccaa agcagccgcg
     1201 ccctctaaaa aatcgtccgc ctgctgcgac gccgcacccg caagggccgc gcccccatcc
     1261 ggtcatcgac ccctgtccca tgggtcccgt tccgtttctt cccggcatct cgatctcctc
     1321 ctttaatgat aataattatc atttgcataa tacgctatcc tgtggcatta tgtcaagggg
     1381 aagatgcata ctcgcgggtc ggcaggcgga gaaaacgccg ggacgtgccg gtacagccgg
     1441 aggggtataa ttttcctcaa aaccagggag ggaacacacc atgacgaaca atacgcgcac
     1501 ggatgagaca gtacaggctt tcctggaaaa gctgaaggac cgggggcttc gcctcactgc
     1561 acagcgtgaa acgctcttcc gggtcatcgc cgaggatttg ggcaacccca cgtcggttca
     1621 gaacatctgg gacaggacgc gcgaggtgga cccctcgatc ggcatcgcga cgatctacag
     1681 gacgctcaac cttctggggg agatgggcgt tgtgaacatc atctacctga gcgacggcga
     1741 attccggctc gacgtcccgc tgcagaagat ccatctcacg gcgttctgca ggcattgcgg
     1801 cgcgctcttc cccctccccg ccgaagatga gaaacagcac actctggaga aatggctgga
     1861 gggaacagga ctcgaactgc ttccgcagtc cgtagccgtg gctgggctct gcacggaatg
     1921 cagggaggcg ctgaaaagcc ccgacgcgct ccccgcccgc agaggcccct tgggacaaca
     1981 gggcccctgc ctccgccgcc gctgccgaag aatggggcgt gttcaggagt gagtcaccgc
     2041 cgaatactac gccggggaaa gcagcgcggg cgcgaccccc ttcagcgcgt gcaaccggag
     2101 cagtcttcat cggcgcactc tttgttcaca agaacaaatt tattataata aaattccgtc
     2161 gcactattcc gccgcgccgc ttgacacttt cggttcattc gctacaatga atgtactttc
     2221 cgtatgaacg ccgcaagaga gtctcctccg caatgagggg agacgccgaa ggggcaaggc
     2281 cgtttcgaaa gaacggctga aactctcagg cgaaaggatt gcggccggac ggtactctga
     2341 aaagaatagt tctcgcaacg accacgtcgt ccctcttcga gactatgcga cagagaatag
     2401 cttacctgta taaggaggcg atttcatggt ctgtcgcgcg ctctcgcacc aaaaccaccc
     2461 cctgaaaatc cgtcgtgtcc gctcgccgga cacggatccg tggaaaaacc tggcccgaga
     2521 ggagttcctc atgggcgact gcgccgacga cgaggcgatc ttctacctct ggcagaatgc
     2581 gcataccgtc gtcatcgggc gcaaccagaa cgcgtggagc gaatgccgcc tcgacctgct
     2641 cgaatccgag ggaggacggc tcgcccggcg ctcctccggc ggcggcgcgg tctaccacga
     2701 tctcggcaac ctcaactact ccttcatcat gccccgccct ctctacagca tggaacggca
     2761 gctcggcgtc gttctcgccg cactgcgctc gctcggaatc aacgccgagt tctccggccg
     2821 gaacgacctc ctgctggagg gacgcaagtt ctcgggaaac gcctaccagc tcacgcggcg
     2881 ccggggactg caccacggca cgctccttct ttccgtggac atgtcgaagc tgtcgcgcta
     2941 cctcaacgtc gaccccgaga agctgcaatc aaagggggta acctccgtgg cctcgcgggt
     3001 ggtcaatctc gcggaggcct cccccgacct gacgcgcgac gcgctgatgg acgcgcttga
     3061 gcgcgcgttc cttgaagagt acccgcacaa cgaggtccgg cgggagtccg acgagcccga
     3121 gtcggaggcc ttcacgcggc tttatgaaaa gtacagcggc agggagtggc gctacggcag
     3181 agtcccggag tgcgcggcgg cgatcaggaa gcgcttcgga tggggcggcg ttgagctgcg
     3241 atgcgacgct cccgcgggaa ggattcgcgg cgcgcaggtc ttctcggacg ccatggacgg
     3301 cgagatgatt ctctcgctgg cgaaggcgct ggagggcctc cccttctcat ggaaggagat
     3361 ggcggaagcg gtgcgcgcgg cgtttcccgg ctcgcccgaa atggcggatg tggcggagtg
     3421 gcttgaagag gcccctatcg gcagacacaa ggaggaaaac gcatgaaaag aaccccgatg
     3481 tacgagaagc acgttgcggc aggcggcagg atggtggatt tcggcggctg ggagctcccc
     3541 gtgcagtacg aagccacggg gatcaagacg gagcacctga ccgtgcgttc caaggcggga
     3601 ctcttcgacg tctcccacat gggggagatc acggtcgagg gacggggcgc gcaagcctgg
     3661 ct
//
LOCUS       DAOPKU010000298         3650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_28193_length_3650_cov_4.613908, whole genome shotgun sequence.
ACCESSION   DAOPKU010000298 DAOPKU010000000
VERSION     DAOPKU010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28193_length_3650_cov_4.613908"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PK849_09560"
     CDS             complement(<1..199)
                     /locus_tag="PK849_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66410.1"
                     /translation="MTTIGNIEHLVDKLCATVSSLRTERDQLRAQVDELKSKFAEKDL
                     ELIRTGKENQRTLEVLEREKLA"
     gene            complement(212..2614)
                     /gene="pheT"
                     /locus_tag="PK849_09565"
     CDS             complement(212..2614)
                     /gene="pheT"
                     /locus_tag="PK849_09565"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPE66411.1"
                     /translation="MLISWNLLNEILTIPASLEEVAERLTFTGCEVESIEYPCKSLKD
                     VRSARIEKLEKHPAKENLFVAVVDDGKGSAVVVTAAPNLSAGDIVPWGRPGAVLADGS
                     VLGTRDFEGVESVGMLLSAAELGIPEAADEFGILRLPAETAPGEDVKHLLGLDDAILD
                     LSVTPNRGDLLSMLGVAREVYALFPEATWKMDLTAFPANAMNAEWPVDFKGITLKDDG
                     CPLYCLGLITGLKSVPSPLRTRILLTLLGMRPISAMVDATNYAMLMLGQPTHAFDADR
                     LPSPEITVRSAADGEETKTLDGKNHSLDPGDMLITSGGLAVGIAGVMGGENSEILEST
                     RTVFLESANFDAIRVSRTSRRLGISSEAAFRYARIVDTRIAQTALKYISSLLEEWGAA
                     EGGYAILSASTKLPEEKSVTLTKGKLKKILLTEDMKEASAILSRLGLVEISSTSETAT
                     YSVPSWRPDISIEEDLIEEVGRIRGYNETLESRLPQALYGRGDIGHTTRLKGEIRSTL
                     LSRGYVELVNYSFLSPSFVELLRLPAADRRARPLLLANPMSVEQSRMRTTLLPGMLRS
                     VEQSVASGWKTAVRVFELGRVFFPLDEGGHEEIERICGVAYGGKDSRLPYGPADADDL
                     FSLKADILALAESRGVVLRFVQGEEHFGHRGQTALLYCGEKAVGYLLRLKPSIEKELD
                     CAPLFAFELDLEPFETSALPEFREPSSFPPVYRDISLFVPLAESMERVAEHIREEGGS
                     LLRDVRLFDMYSGKGVPEGFRGLAFSLAYKRDDRTLTDKEVDEVNERVRSRMSSSGYV
                     LR"
     gene            complement(2608..3645)
                     /gene="pheS"
                     /locus_tag="PK849_09570"
     CDS             complement(2608..3645)
                     /gene="pheS"
                     /locus_tag="PK849_09570"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPE66412.1"
                     /translation="MQTELESIEQRFTEQLSGIASSEELQQVKVAFLGKKGAVTSFLK
                     TLGSLSPEERPVAGQAVNSLRDRMEAILDEKKSFLEEKESDAAERAYRIDVTLPPRGR
                     SIGGIHPVAQLTQDVVEILAGLGFSVALGPEIEDDFHNFEALNIPASHPARDMQDTFY
                     FPDGKLLRTHTSPVQVRSMLRYGAPLRIVCPGKVYRRDSDPTHSPMFNQLEGLLVEKD
                     ISVADMKGCLETLMAAIFSRPLKARYRASYFPFTEPSLELDIECVECSGKNPSCRICK
                     GTGWLEVAGLGMVHPNVIRSGGIDPDVYNGFAWGIGLDRIAMLKYRLTDLRVLFEGNV
                     PYLLSGRRFTC"
BASE COUNT          731 a         1103 c         1069 g          747 t
ORIGIN      
        1 gggcaagctt ctccctttcg agcacctcca gcgtccgctg attttccttc cccgtcctga
       61 tcagttcgag atccttctcg gcaaatttgc ttttgagctc gtcgacctgg gcgcgaagct
      121 ggtcgcgctc ggtccgcagc gacgacacgg tcgcgcagag cttgtcgacc agatgctcga
      181 tgttgccgat cgtcgtcatg cagccccctc cttatcgaag gacgtatccg gacgagctca
      241 ttctgctgcg aactcgctcg ttgacttcgt ccacttcctt gtcggtgagc gtcctgtcat
      301 cccgtttgta tgcgagagag aacgccagcc cccggaagcc ttcgggaact cctttgcctg
      361 agtacatgtc gaagaggcgc acgtcgcgga ggagtgagcc tccttcctcc cggatgtgct
      421 ccgccacccg ctccatcgac tccgccagcg ggacgaagag ggagatatcc ctgtagaccg
      481 gagggaaaga agaaggttct ctgaattcag ggagcgcaga cgtttcaaac ggctccagat
      541 ccagttcaaa agcaaagagc ggagcgcagt caagctcctt ttcgatcgac ggcttgaggc
      601 gaagcagata tcccacggcc ttctctccgc agtacaggag cgcggtctgc cccctgtggc
      661 cgaagtgctc ctctccctgg acgaaacgga gaacaacgcc gcggctctcg gcgagggcga
      721 ggatatccgc tttgagcgaa aagagatcgt ccgcgtcggc cggtccgtac ggaagccggg
      781 agtcctttcc gccgtaggcg acgccgcata tacgttcaat ctcctcgtgc ccgccttcat
      841 caagaggaaa gaagaccctg ccgagctcaa agacgcgcac cgcggtcttc caccctgaag
      901 caacgctctg ctcgacgctt cgaagcattc cgggaagaag cgtcgtacgc atcctcgact
      961 gctccacgct catcgggttg gccagcaaca gcgggcgggc cctgcgatcc gcggccggga
     1021 gacgaagtaa ctcgacgaac gaaggggaga gaaagctgta gttcacgagc tccacgtatc
     1081 ccctggagag cagcgtcgat cgtatctctc ccttgagtct ggtcgtgtgg ccgatgtctc
     1141 ctcgcccgta gagcgcctgc ggaagacggg actccagcgt ttcgttgtac ccgcggatcc
     1201 tgccgacctc ttcgatgagg tcctcctcga tggagatgtc gggacgccac gagggaacgg
     1261 aatatgtcgc cgtttcggac gtcgaggaaa tttcgacgag gccgaggcgg gagagtatcg
     1321 cggacgcctc tttcatatcc tccgtgagaa ggatcttctt caattttccc ttggtcagcg
     1381 tcacagactt ctcctcggga agcttcgtcg acgcggagag gatggcgtac cctccttccg
     1441 cggcgcccca ctcctccaga agcgaggaga tatacttcag ggccgtttgc gcgatgcgcg
     1501 tatcgacgat acgcgcatac cggaaggcgg cctcggaaga gataccgagt ctgcgggagg
     1561 ttctgcttac cctgatggca tcgaaattgg ccgactcaag gaagacggtt ctcgtggact
     1621 caaggatctc gctgttctct cctcccatca cgccggcgat gcctacggcg aggccgccgc
     1681 ttgtgatgag catatcgcct gggtcaagac tgtgattctt tccgtcgagc gttttggttt
     1741 cttcgccgtc ggcggcggag cgaacggtaa tctccggcga cggaagacgg tcggcgtcaa
     1801 aggcgtgcgt cggctgaccg agcatgagca tggcgtagtt ggtcgcatcg accattgcgg
     1861 agatcggacg cattccaagc agcgtgagga gaatgcgcgt cctcaacggc gagggaacgc
     1921 ttttcaggcc cgtgatcagg ccgaggcagt agagcgggca tccgtcgtct ttcaaggtaa
     1981 tccccttgaa atcaaccggc cactcggcat tcatggcgtt cgctgggaaa gctgtaagat
     2041 ccattttcca cgtggcctcg gggaagagcg cgtacacttc gcgcgccact ccgagcatgc
     2101 tcagcagatc tcctctgttc ggcgttaccg aaaggtcgag gattgcgtcg tcaagcccca
     2161 gaagatgttt gacatcctct ccgggcgcgg tctccgcagg caaacggaga atgccgaatt
     2221 catcggccgc ctcaggtatg cccagttccg cggccgagag cagcatcccc acgctctcga
     2281 cgccttcaaa gtccctcgtg ccgagaacgg aaccgtcggc gagcacagcg cccggacgcc
     2341 cccatggaac aatatcccct gcggaaaggt tcggcgccgc cgttacgacg accgctgagc
     2401 ccttcccgtc gtccacaacg gctacgaaga ggttttcttt cgccgggtgt ttttcaagct
     2461 tctcgatacg cgcagagcgc acatccttca aagatttgca tggatattct atcgattcga
     2521 cttcgcaacc cgtgaaagtc aaccgttccg cgacctcttc aagcgacgcg ggaatcgtca
     2581 gaatttcgtt cagcagattc caggaaatca gcatgtgaaa cgcctccccg agagaagata
     2641 gggcacgttg ccttcaaaga gaacgcgcaa atcggtcagc cggtatttga gcatggcgat
     2701 gcggtcgagg ccgattcccc aggcaaaacc gttgtacaca tccggatcga tgcctccgga
     2761 acggataacg ttcggatgca ccatgccgag acccgcgact tcgagccacc cagtcccttt
     2821 gcagattctg cacgaggggt tcttgccgga acactcgacg cactcgatgt cgagttcgag
     2881 cgacggctcg gtgaagggga agtagctcgc ccggtagcgc gctttgaggg ggcgggaaaa
     2941 aatcgccgcc atgagcgttt caaggcaccc cttcatgtcg gccacggaga tgtccttctc
     3001 tacgagcagg ccttcgagct ggttgaacat gggcgagtgc gtggggtcgc tgtcgcgccg
     3061 gtacaccttg ccggggcaga caattcgcaa cggagctccg tagcggagca tcgaccgtac
     3121 ctgcacaggc gacgtatgtg tccgcagcag ttttccgtcc gggaaataga acgtgtcctg
     3181 catatccctt gcagggtggg acgccgggat gttgagcgcc tcgaagttgt gaaagtcgtc
     3241 ctctatctcg ggaccgaggg ctacggaaaa accaagcccc gcgagaatct ccaccacatc
     3301 ttgcgtcagc tgcgcgaccg ggtgaattcc gccgatgctc ctccccctcg ggggcagagt
     3361 gacgtcgatg cggtatgccc gctccgcggc gtcggattcc ttctcctcga ggaagctctt
     3421 cttttcgtct aaaattgctt ccattctgtc gcggagggag ttcaccgcct gcccggcaac
     3481 cgggcgttcc tccggagaaa gggaaccaag cgttttcaaa aaggaggtca cggcgccctt
     3541 cttgccgaga aaagcgacct tcacctgctg aagttcctcg gaagaagcga ttcccgatag
     3601 ttgttccgta aaacgttgtt caatgctttc cagttcagtc tgaatggatg
//
LOCUS       DAOPKU010000299         3632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_28379_length_3632_cov_8.570310, whole genome shotgun sequence.
ACCESSION   DAOPKU010000299 DAOPKU010000000
VERSION     DAOPKU010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28379_length_3632_cov_8.570310"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PK849_09575"
     CDS             complement(<1..892)
                     /locus_tag="PK849_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPE66413.1"
                     /translation="MEIYMQAIREIIVSSGFAGLTGGHLMMIVVALVMLYLAIGKGFE
                     PLLLVPIGFGCLLVNLPLSGIMDDGGFLRYVFFGTEHEIYPVIIFMGIGALTDFAPLL
                     ANPITFLLGAAAQFGVFIALFGAMLMGFSVNEAASIAIIGGADGPTSIYLTMKLAPQI
                     LGAVAVAAYSYMSLVPLIQPPVIKMLTTKADRKIRMDTLRPVTKLEKILFPIVTTMAA
                     GLILPASVPLVGILMFGNLVRECGVTERLSQAAQNEILNTTTIFLGLSVGATMNAEEF
                     LTVATLKIILLGLVAFIFSTA"
     gene            complement(949..1356)
                     /locus_tag="PK849_09580"
     CDS             complement(949..1356)
                     /locus_tag="PK849_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPE66414.1"
                     /translation="MSKKYRITVDGTAYTVEVEDLGAGAPAASAPAAAAAPAPAPVAA
                     PAPAAAPAPAAAAPAPAAGGAGVPVTAPMPGKVLKVTVSAGAAVKNGDLVLVLEAMKM
                     ENEIFSPADGVVKEIRSREGDAVNSGDVMMVVG"
     gene            complement(1568..2047)
                     /locus_tag="PK849_09585"
     CDS             complement(1568..2047)
                     /locus_tag="PK849_09585"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPE66415.1"
                     /translation="MSIAPAATAVTETVDAAAAAASASARIAEEMSGVGGALTMSIIA
                     FSVVFLVLGGLSGVIYAIKYMAQALERKKNEPPAGNATAGNAAAPAPVGAAPAAQSGA
                     DGRLMAVLAAAVAAAGIGGRITGVTVAGGSRARPARAAGWRSASLAEGIQSLSRDWK"
     gene            complement(2073..>3632)
                     /locus_tag="PK849_09590"
     CDS             complement(2073..>3632)
                     /locus_tag="PK849_09590"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPE66416.1"
                     /translation="MSQRTIEELCEELLLKREKAQHGGGEKAVAKQREKGKGTARERI
                     EKLLDPGTFIEVDEFVRHRCVNFGLEKTVFDGDGVIIGWGLVEGRKVYVYSQDFTVLG
                     GSLGEMHAAKICKIMDMAMSTGCPVVGINDSGGARIQEAVDSLNGYGAIFSRNTIASG
                     VIPQLSVIAGPTAGGAVYSPALTDYIFMVEGTGIMHITGPAVIKAVTGEEVTSEQIGG
                     SMANNSKSGNAHFSAKSEDECIAQVRKLLSYLPQNNLEEAPEKETGDDPLREDAELRN
                     IVPVNPNKGYDVREVIRRVVDESDFFEVQPLWAKNILTGYARLGGKVIGIIANQANHL
                     AGCLDIDASDKASRFIRHCDAFNIPVVTFVDVPGYLPGTAQEFGGIIRHGAKMLYAYS
                     EATVPLITVVLRKAYGGAYLGMCSKALGADIVLAWPQAEIAVMGAEGAANIVFRREIE
                     AAEDSVATRTRKIEEYREAFANPYVAAERGLVDRVILPEATRGEIWRALVGAESKRDL
                     RPRRKHGVMPH"
BASE COUNT          515 a         1125 c         1125 g          867 t
ORIGIN      
        1 ccgccgtgct gaatatgaac gctacaagtc ccagcaatat gatcttcagc gtcgccactg
       61 tcagaaactc ctcggcgttc atggtcgctc ccaccgacag tcccaggaag atcgtcgtgg
      121 tgttcaggat ctcgttctgc gccgcctggc tcagccgttc cgtgaccccg cattcacgca
      181 cgaggttccc gaacatgagg atgcccacca gcggcaccga cgcggggagg atcaggcccg
      241 ccgccatcgt cgtcactatc ggaaagagga tcttctcaag cttcgtgacg ggccgcagcg
      301 tgtccattcg aatcttccgg tcggccttgg tcgtgagcat cttgatgacc ggcggctgga
      361 tgagcggcac gagcgacatg tagctgtacg ccgccacagc cacggctccc aggatctgcg
      421 gcgcgagctt catggtgagg tagatgctcg tggggccgtc cgctcctcct atgatggcga
      481 tcgacgcggc ttcgttgacg gagaatccca tcagcatggc gccgaagagc gcgatgaaga
      541 ctccgaactg cgccgccgct ccgagcaaaa acgtgatggg gttggccaac agcggcgcga
      601 agtcggtcaa ggcgcctatt cccatgaaga tgatgacggg gtagatttcg tgttccgttc
      661 cgaagaagac gtaccgcagg aaccctccgt cgtccattat ccccgacagc ggcaggttca
      721 ccagcagaca gccgaatccg atcggtacga gcagcagcgg ttcgaatcct ttccctatgg
      781 cgaggtagag catcaccagc gccacgacga tcatcatgag gtgtcctccg gtgagtccgg
      841 cgaatccgga cgatacgatg atctctctta tcgcttgcat gtagatttcc attgccctcg
      901 gtctccctcc ttgctcctgt ctctacaggt tgtgtcctgg tcccggggct accctacgac
      961 catcatgacg tcgccggagt tgacggcgtc tccttcccgg ctccggatct ccttgacgac
     1021 tccgtcggcg ggggagaaga tttcgttctc catcttcatg gcttcgagga cgaggacgag
     1081 gtctccgttc ttgaccgcgg ctccggcgct tacggttact ttgaggactt tgccgggcat
     1141 gggggcggtg acggggactc cggcgcctcc tgctgcgggc gcgggggctg ccgccgcagg
     1201 ggcgggggcg gctgccggtg cgggcgccgc taccggggcc ggggccggag ctgccgcagc
     1261 tgccggggcc gacgccgcag gggcgcccgc tccgaggtct tccacttcta ctgtgtaggc
     1321 tgttccgtcg actgtgattc tgtatttttt gctcattgtg cgatgagcct ccttctttcg
     1381 tttggtttcg agtgttcgtt gtgtgttgcc gttctttatt cgtctcttgg cttttctgct
     1441 tttcgcttgc tttttaatgc ttttaatact tctaatactt ctaatacttc tttattgctt
     1501 ctttattgct tctttattgc ttctttattg cttcttcgct tctccgggcg tgcgcgcggc
     1561 tgcggcgcta tttccagtcc cgggagaggc tctggattcc ttcggcgagc gaggcgcttc
     1621 tccatccggc cgctcgggcg gggcgggcgc ggctgcctcc ggctaccgtc actccggtga
     1681 tgcggccgcc gattcctgcg gcggctaccg cggcggcgag cactgccatg agacgaccgt
     1741 cggctccgct ctgcgccgcg ggggcggcgc cgacgggcgc cggcgctgcg gcgtttcctg
     1801 ctgtcgcgtt tcctgcgggc ggttcgttct tcttccgttc cagcgcctgc gccatgtact
     1861 tgatggcgta gatgactccg ctcagccctc caagtaccaa aaagacgacg ctgaaggcga
     1921 ttatggacat ggtgagcgct cctccgactc cgctcatctc ctccgctatg cgcgcggagg
     1981 ctgacgcggc tgcggcggct gcgtcgactg tctcggttac ggcggtcgct gcgggtgcga
     2041 tgctcattcg tgcgtcactc cttccttggt atttagtgcg gcatgacgcc gtgcttgcgc
     2101 cgcgggcgca ggtcgcgctt gctctccgct cctacgagcg ctctccagat ctcgccgcgc
     2161 gtggcttcgg gcaggatgac acggtcgacg aggcctcgtt cggcggcgac gtaggggttg
     2221 gcgaaggctt cacggtattc ttctattttc ctggtgcgcg tggcgacgct gtcttccgct
     2281 gcttcgatct cgcgtcggaa gacgatgttc gcggcgcctt cggctcccat gacggcgatt
     2341 tcggcctgcg gccatgccaa tacgatgtcg gctcccaggg ctttgctgca catgccgagg
     2401 tacgcgcctc cgtacgcttt cctcaatacg acggtgatga gggggacggt ggcttcgctg
     2461 tacgcgtaga gcattttggc gccgtggcgg atgatgcctc cgaattcctg cgctgtgccg
     2521 gggaggtatc cggggacgtc gacgaaggtg acgacgggga tgttgaaggc gtcgcagtgg
     2581 cggatgaacc ggctggcttt gtccgaggcg tcgatgtcga ggcatccggc gaggtggttg
     2641 gcctggttgg cgatgatgcc gatgactttg cctccgagac gcgcgtatcc ggtgaggatg
     2701 ttcttggccc acagcggctg gacttcgaag aagtcgcttt cgtcgacgac tctccggatg
     2761 acttccctca cgtcgtaccc tttgttgggg ttgacgggga cgatgttcct cagttcggcg
     2821 tcttctctga gggggtcgtc tcccgtctct ttctcgggcg cttcttccaa attgttctgc
     2881 gggaggtagc tcaggagctt cctgacctgc gcgatgcatt cgtcttcgct cttggcgctg
     2941 aagtgcgcgt tcccgctctt gctgttgttg gccatggagc cgcctatctg ttcgctggtg
     3001 acttcttcgc cggtgacggc tttgatgacg gcggggccgg tgatgtgcat gattcccgtt
     3061 ccttcgacca tgaagatgta gtcggtgagc gcggggctgt acacggcgcc tccggcggtg
     3121 gggccggcta tgacggagag ctgcgggatg acgccgctcg ctatggtgtt ccgggagaag
     3181 atcgctccgt acccgttgag cgagtcgacg gcttcctgga tcctcgctcc gccgctgtcg
     3241 ttgatgccga ccacgggaca tccggtgctc atggccatgt ccatgatctt gcagatcttc
     3301 gcggcgtgca tctccccgag cgacccgcca agtactgtga agtcctggct gtagacgtac
     3361 actttgcgcc cttccaccag tccccacccg atgatgactc cgtctccgtc gaagaccgtc
     3421 ttctccagtc cgaagttcac gcaccggtgc cggacgaatt cgtccacttc gatgaacgta
     3481 ccggggtcga gcagcttctc tatacgctcc cgcgccgttc ccttcccctt ctcacgctgc
     3541 ttggcgaccg ctttctcacc gcctccatgc tgcgctttct cacgcttcag caacagctcc
     3601 tcgcagagct cttctatcgt tcgctgcgac at
//
LOCUS       DAOPKU010000300         3627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_28439_length_3627_cov_5.157895, whole genome shotgun sequence.
ACCESSION   DAOPKU010000300 DAOPKU010000000
VERSION     DAOPKU010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28439_length_3627_cov_5.157895"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PK849_09595"
     CDS             <1..647
                     /locus_tag="PK849_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66417.1"
                     /translation="LFMPLSLPLPEASLFPLTSVALLIGAAVTLCAIRCGKDREDCAI
                     TFRFFLLCFTYLGIVWLLLPAAETRLRLALLSAGILLIFLAASLLPFRRRMAPFIMLL
                     LLCVVTWRELPAYSQNLGRWSDALRGEGAISGLYPSEESIGRYLHDRLSGIEVGLDGR
                     APVVEFFAGGRVRFVPPYEQPLPEVVLRPSFTPLSGGDNVLLRGERWTLLQRAR"
     gene            728..1831
                     /locus_tag="PK849_09600"
     CDS             728..1831
                     /locus_tag="PK849_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66418.1"
                     /translation="MAFPLWRRARCCFVLLLLLCMIFSGTAGAALLRDLESAVRLRSG
                     SGIPVRQSGDGYAVDAALFLLYAVESGNRALFDELLPPFRQAFIVPFEAGAHTSGTVG
                     WKRRGKESPDASGTTETMLAAKALWRAGVLWKNESFLEESLSLIRGYLAHESGKEKSW
                     QIRNYYNYGTRAFAVNSYALGYFPDFLFEVARSGLLPEASKAAERSLRFLRNAALPNG
                     LFSSSFDPELRTLFPEAEFSYHFSPDFRIKPGDSAFIALEMAESDPGISRRTLALLKV
                     FWPSLPDTVYGFLSLELSRAERRPEISTGHLAGVYRLALRFDEAFAREILPVLNERAL
                     SLLSGNEEEAWTWDAVNMLLALLDGERPGELAR"
     gene            complement(1935..2927)
                     /locus_tag="PK849_09605"
     CDS             complement(1935..2927)
                     /locus_tag="PK849_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPE66419.1"
                     /translation="MKMRTGCVAMLLVALFASAAFGAQFTINAGIGLNDKSAQFQSLQ
                     YFKEVVEKNSDGKIEVVLFHSSQLGDDRNMMEALKMGTQEMTCPSTAPMTAFINAFKI
                     YDLPFIFANEEVADYILDGPVGKKLLDMLPDQGLVGLAYWENGFRMLTNSSRPVQSPE
                     DLKGMKIRTMENPIHLAAFRTMGANPTPMAFGELFSAMQQKVVDGQENPWGTIFLQNF
                     FEVQKYATDTGHVYSPFVLLISKKFWDKLPDDLKAVVQDAADQSKVHNRALNRKMNAE
                     YLEKLKEKMDVVILTPEQKAAFQKACQPIYEQFGKDIGEDLIKEVQELSAAFKK"
     gene            complement(3033..>3627)
                     /locus_tag="PK849_09610"
     CDS             complement(3033..>3627)
                     /locus_tag="PK849_09610"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE66420.1"
                     /translation="ILGGIFTATEAGAISAFVAMILGLFVYKEISFKDLPGIFLSTGK
                     TTATVLYHCGMAMCTAWLLTRARVPFELAAMVQSITDSQLGVLLMTNVLLFLVGFVMD
                     LTPAMLILAPILLPVMQQVGVDPVYFGVIMSVNLGIGLITPPVGTVLYVATGVANVKF
                     EELVKSIAPFVVAELAVLILLIFFPQIVMFIPNLVLP"
BASE COUNT          638 a         1037 c         1125 g          827 t
ORIGIN      
        1 gtctctttat gccgctctct ctgcctcttc cggaggcgtc gctcttccct ctcacctccg
       61 tcgcgctgct gatcggggca gccgtgacgc tctgcgcgat acgctgcgga aaggaccggg
      121 aggattgcgc gatcactttc cggttcttcc tgctctgttt tacgtatctc gggattgtct
      181 ggcttcttct tcccgccgcg gagacgcggc tccggctggc gttactctcc gccggcatcc
      241 tgcttatttt tcttgccgct tccctgctgc cgtttcgccg ccgcatggcg ccgttcatca
      301 tgctgctgct tctctgcgtc gtcacgtggc gggagctgcc tgcgtattcg cagaatctgg
      361 ggcgatggag cgacgccctt cgaggagagg gggcgatctc cggcctgtac ccttcggagg
      421 agagcatcgg ccgatatctg catgatcggc tctccggtat cgaggtcggt ctggacggac
      481 gcgctccggt ggtggagttt ttcgccgggg ggagggtacg ctttgtcccg ccgtatgagc
      541 agcctcttcc tgaagttgtt ctgcggccct cctttacacc gctatccggc ggtgacaacg
      601 tgctgctgcg gggcgaacga tggactcttc tgcaaagggc gcgctgaatg tgttcgcagg
      661 tgttttccct ctccggcggt acggtttcaa ccttgagccg cccttagagc aggggagggg
      721 ggccgctgtg gcgtttcctt tatggagaag agcgcgctgc tgtttcgttc tcttgctgct
      781 attgtgcatg attttctccg gaactgccgg agctgctctt ctgcgcgatc tggaatcggc
      841 ggtgcggctt cggagcggct ccggaattcc tgtgaggcaa agcggcgatg ggtatgctgt
      901 ggacgccgcg ctctttcttc tctatgcggt cgaatcagga aaccgcgcgc tcttcgacga
      961 gctgcttcca ccctttcgtc aggccttcat cgttccgttc gaggcgggag cgcatacgag
     1021 cggcacggtc ggatggaaga gaagagggaa ggagtctccc gacgcctcgg gaacgaccga
     1081 gacgatgctc gcggccaagg cgctctggcg cgccggcgtt ttgtggaaga atgaatcgtt
     1141 tctcgaagag agcctttcgc tgatacgcgg ctatcttgcc cacgagtccg gcaaggagaa
     1201 gagctggcag atccgaaact actacaacta cggtacgcga gccttcgccg tgaacagcta
     1261 cgctctcggg tatttccccg actttttgtt cgaagtggcg aggagcgggt tgcttccgga
     1321 agcgtcgaag gccgcggagc gttctcttcg ctttctccgg aacgccgcgc tgccgaacgg
     1381 ccttttttcc agctctttcg accctgagtt acgcacgctt tttcccgagg ccgagttttc
     1441 atatcacttt tctcccgact tccgcatcaa accgggggat tccgcgttca tcgcgttgga
     1501 gatggcggag agcgatccgg gtatttcccg cagaacgctc gcgctgttga aggttttttg
     1561 gccctctctt cccgataccg tctacggctt tctttctctt gaactctcaa gggcggagag
     1621 acggccggag atttccaccg gacacctggc gggggtctac cgtcttgcgc ttcgcttcga
     1681 cgaagcgttc gcgcgcgaga tactgccggt gctgaacgaa cgcgcgcttt ctctgctctc
     1741 cgggaacgaa gaagaagcgt ggacctggga cgcggtgaac atgctgctgg cgcttcttga
     1801 cggggaacgt cccggggaac tggctcggta agcgggagcc ccccaacgta ttgaacgcga
     1861 tgcgagcgaa aagaacgcgc cgactccccg gtttcggaga gcggcgcgtt tgttgttttt
     1921 gggacgaatc agggttactt cttgaacgcg gcggagagtt cctgcacttc cttgatcagg
     1981 tcctcgccga tatcttttcc aaactgttcg tagataggct ggcaggcttt ctggaacgcg
     2041 gccttctgtt ccggggtgag gatgacgacg tccatcttct ccttcagctt ttccaggtat
     2101 tcggcgttca tcttccggtt gagcgccctg ttgtgtacct tcgactggtc tgcggcgtcc
     2161 tggacgacgg ctttcaggtc gtcggggagc ttatcccaga atttcttcga gatcagcagg
     2221 acgaaggggg agtagacgtg ccccgtgtcc gtggcgtact tctgcacttc gaagaagttc
     2281 tgcaggaaga tggtgcccca gggattctcc tgtccgtcga caaccttctg ctgcatggcg
     2341 gagaagagtt cgccgaaggc catcggcgtc gggttggcgc ccatcgtgcg gaaggcggca
     2401 aggtggatgg ggttctccat ggtgcggatc ttcatcccct tgagatcctc gggcgactgg
     2461 acggggcggg agctgttcgt gagcatgcgg aagccgttct cccagtaggc cagaccgacg
     2521 agcccctggt cggggagcat gtcgagcagc tttttcccca cagggccgtc gagaatgtaa
     2581 tccgcgacct cttcattggc gaaaatgaag gggaggtcgt agatcttgaa cgcgttgatg
     2641 aaggccgtca tcggagcggt ggaggggcag gtcatctcct gcgttcccat cttgagcgct
     2701 tccatcatgt tacggtcgtc gccgagctgg ctggagtgga agaggacgac ctcgatcttt
     2761 ccgtccgagt tcttctcgac aacctctttg aaatactgga gcgactggaa ctgggcggac
     2821 ttgtcgttca gtccgatgcc cgcgttgatg gtgaactgcg ccccgaaggc cgccgaggcg
     2881 aagagtgcga caagaagcat ggcaacgcaa ccggttctca ttttcatctg tttctctccc
     2941 ttcatgtttt caaaatgtat tcttcttcaa aacagtaccc gtggtcgact ctgtccgcac
     3001 ccccttccgg atgtctgaaa aaatgacgcg ctctacggaa gcacaagatt cgggatgaac
     3061 atgacgatct gcgggaagaa gatgagcagg atgagcaccg ccagctccgc gacgacgaag
     3121 ggagcgatgg actttaccag ctcctcgaac tttacgttcg ccacgccggt cgccacgtag
     3181 aggaccgttc ccaccggcgg tgtgatgagc ccgataccga ggttcaccga catgatcacg
     3241 ccgaagtaaa ccggatcgac ccccacctgc tgcatcaccg gaagaaggat cggcgcgagg
     3301 atgagcatcg ccggggtaag atccatgacg aagccgacga ggaagagaag cacgttcgtc
     3361 atcaggagga cgccgagctg cgagtcggtg atcgactgga ccatcgcggc gagctcgaag
     3421 ggaaccctcg cgcgggtgag cagccacgca gtgcacatcg ccataccgca gtggtagagc
     3481 acagtggcgg tcgtcttgcc tgtcgacaaa aagatgccgg gcaggtcttt gaacgatatc
     3541 tccttgtaga cgaagagccc gagaatcatg gcgacgaacg ccgagattgc gcccgcctcc
     3601 gtcgcggtga agatgccgcc gagaatg
//
LOCUS       DAOPKU010000301         3618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_28533_length_3618_cov_5.026663, whole genome shotgun sequence.
ACCESSION   DAOPKU010000301 DAOPKU010000000
VERSION     DAOPKU010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28533_length_3618_cov_5.026663"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PK849_09615"
     CDS             complement(<1..699)
                     /locus_tag="PK849_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66421.1"
                     /translation="MRHLFTRWSFWSLLLLVLTALFGGRLFDAPPGREVAAAFSKPLW
                     IDRDLPPTKQLRLTADSPSLRFDWQYAPPSGLSVRIDPSVGQTGRFVEWRKPGAPEPL
                     RLAPLRGGFEADGRDIAFKRSLGLPPFGDAAASLFAGQGSYELSLSGEGNFEGEVVLK
                     LEGGRWGLLGTDQRGRDVAALFVAGVRVSLIVGIFATLLATFLGTSFGLAAGYLGGWT
                     DSILMRIVDLLLSIP"
     gene            complement(699..1670)
                     /locus_tag="PK849_09620"
     CDS             complement(699..1670)
                     /locus_tag="PK849_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66422.1"
                     /translation="MNYWGRRISGALLVLALVLVLNFLLFRLMPGDPVASIVDPRFSP
                     EAKEQLRRMYGLDQPLPVQFALYVKSTLTFDFGISFLTGLPVAEELRSRVPNTVILLG
                     TSLLLSAALGTWLGMKAALRRGSLLERCVLWSGALSFSFPSFFVQLLLLMFFASMIPL
                     FPLRGTLSVPPPAGGPALLFDYLRHMTLPVFSLVLLGFGGWAIYVRNLTVKILGEDFI
                     LLARARGLPKKNILRGHVFRTMLPPLLTIFLLSLPGVVSGAVITETVFSLHGVGRFLL
                     EAVTGHDYPAAGAAFFLLSLLTVTCNLLSDLLYGLTDPRVRLERGRR"
     gene            complement(1678..3351)
                     /locus_tag="PK849_09625"
     CDS             complement(1678..3351)
                     /locus_tag="PK849_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66423.1"
                     /translation="MKRPIAFALVLTIVCFLSGSAWSANGKFNAESYSSPKIRDLYLV
                     IIRDPDAQLLALQRGQADILGDLHRPVDVDTLADDPRVNLSLASGFHGFFLGFNVRTF
                     PWNRLELRQAAWQALPRERVVRDLFAGYAEPLSTFLPPVSPYYEPNVAAYPFDPDAAK
                     KRLADAGWTWGEDGVLISPEGEKIPDQRLLSPTASVAPTTAELAARAADALSAIGIPV
                     RSEPMDFSTMLSRLDERDFQMYVLAWQMTRDPDSLYAFYSTKMDIPGGYNLPGISDPE
                     LDAVLDRLRFAPDAMTALDAASDAQKKLAELAPVAPIYSRYTISAVRRDWKGTLVTDR
                     TTADNLWSLLSMEPAAGPMRPLYLVQGEEPRALNPFTSSSAYDWQVLGLLYDSFIAID
                     PYTLETIPWLAKSWTVTTKTRDDGLHTVLTFHLRDNVRWQDGKPMTAEDAAFTYEYLR
                     KNMIPRYFDNVDNIEKIETPDPLTLVVTMKNTSFWHLHRVGGIPVLPRHIVEKVEDWR
                     SWLPASLKHPEDDSLTQLVGTGPFVFREYRAGEYVHLTANDAFWLGRKR"
BASE COUNT          715 a         1092 c         1230 g          581 t
ORIGIN      
        1 cgggatggag agcagcaggt cgacgatgcg catcaggatg ctgtcggtcc accctccgag
       61 gtagcctgcc gcgagtccga aggaggtccc gaggaaggtc gccaggaggg tagcgaagat
      121 gccgacgatg agcgagaccc gtacccccgc gacgaagagc gcggcgacgt cccttccgcg
      181 ctggtcggtt ccaaggaggc cccagcgccc tccttcgagt ttgaggacaa cctccccttc
      241 gaagttgcct tcgccggaga gggagagttc atagctcccc tgtccggcga aaagggacgc
      301 agcggcgtcg ccgaatgggg gaagtccaag ggagcgcttg aaggcgatgt cgcggccgtc
      361 cgcctcgaag ccccccctga gaggagcgag acgcaacggc tccggagcgc ccggcttccg
      421 ccattcgaca aaacgcccgg tctgccctac cgagggatcg atccgcacgg agagccccga
      481 gggaggagcg tactgccagt cgaaacgaag cgagggggaa tccgccgtga gccggagctg
      541 cttcgtcggc ggcaggtcgc ggtcgatcca gagaggcttc gagaaggccg ccgccacctc
      601 ccgtccgggg ggcgcgtcga agaggcgtcc tccgaaaaga gccgtcaaaa ccagaagcag
      661 cagagaccag aagctccagc gagtgaaaag gtgtcgcatc agcggcgtcc cctttccagg
      721 cgcaccctgg ggtcggtgag gccgtagagc aggtcggaga gcaggttgca cgtcacggtg
      781 agcagggaaa gcagaaagaa ggccgcgccg gcggcggggt agtcgtgccc ggttaccgcc
      841 tccagcagga agcgtcccac gccgtggagc gagaagacag tctccgtgat cacggcgccc
      901 gagacgacgc ccggaagcga gagcaggaag atggtgagca ggggcgggag catcgttctg
      961 aagacgtgtc cgcgaaggat gtttttcttc ggcaggccgc gcgccctcgc gaggagaatg
     1021 aagtcctcgc cgaggatctt cacggtcagg ttgcgcacgt agatcgccca cccgccgaac
     1081 ccgagcagca cgagcgagaa gacgggcagc gtcatgtgcc gcaggtagtc gaacagcagc
     1141 gcgggacctc cagcgggcgg agggacggag agcgtcccgc gaagaggaaa gagcggaatc
     1201 atcgaggcga agaacatcag cagcagcagc tgaacgaaaa acgagggaaa ggagaaggag
     1261 agcgcgccgc tccagaggac gcagcgttcg aggaggctgc cccgccgcag cgccgctttc
     1321 atgccgagcc aggttcccag ggcggcggag agcaaaaggc tcgtcccgag cagtatcact
     1381 gtgttcggta ctcgggagcg cagctcctcg gccaccggca gccccgtcag gaaggagatg
     1441 ccgaagtcga aggtcagcgt cgacttcacg tagagcgcaa actgcacggg gagcggctgg
     1501 tcgagtccgt acatccgccg cagctgctcc ttcgcctcgg gcgagaaacg ggggtccaca
     1561 atggaagcca cggggtcgcc cggcatgagg cggaagagca ggaaattcag gacgagtacg
     1621 agcgccagga caagcagcgc tcccgagatt ctccttcccc agtagttcac cggcgtctta
     1681 ccttttccgc ccgagccaga aggcgtcgtt cgccgtcaga tggacgtact cgcccgcccg
     1741 gtactcgcgg aagacgaacg ggcccgtccc cacgagctgc gtcagggagt cgtcctccgg
     1801 atgcttcagc gaggcgggca gccagcttcg ccagtcctcc accttctcca cgatatgccg
     1861 cggaagcacc ggaataccgc cgacccggtg cagatgccag aagctcgtgt ttttcatcgt
     1921 cacgaccagc gtcagcgggt cgggggtttc gatcttctcg atgttgtcca cgttgtcgaa
     1981 ataccgtgga atcatgttct tccgcaagta ttcataggta aaggcggcgt cctctgccgt
     2041 catcggtttc ccgtcctgcc agcgcacgtt gtcgcgcaga tggaaggtga gcacggtatg
     2101 cagtccgtcg tcccgtgttt ttgtcgttac ggtccatgat ttggcgagcc atggaatcgt
     2161 ctccagcgtg taggggtcga ttgcgatgaa ggagtcgtag aggagcccga gcacctgcca
     2221 gtcgtaggcg gagctggatg tgaacgggtt gagcgctcgc ggttcctccc cctggaccag
     2281 gtagagcggg cgcatcggcc ccgcggccgg ctccatggag agcaggctcc acaggttgtc
     2341 cgccgtggtc cggtccgtga ccagcgttcc cttccagtcc cggcgcacgg cggagatggt
     2401 gtagcggctg tagatcggcg cgacgggggc gagctccgcc agcttcttct gggcgtcgga
     2461 ggccgcgtcg agcgccgtca tcgcgtcggg cgcgaaacgg agccggtcca gcacggcgtc
     2521 cagttcgggg tcggagatgc cggggaggtt gtatccgccg ggtatgtcca tcttggtcga
     2581 gtagaacgcg taaagagagt cggggtcgcg cgtcatctgc cacgcgagga cgtacatctg
     2641 gaagtcccgc tcgtcgagcc gcgagagcat ggtggagaag tccatcggct ccgaacgcac
     2701 aggaatgccg atcgccgaaa gcgcgtccgc agcccgcgcc gcgagctccg cagtggttgg
     2761 cgcaacggag gccgtcggcg agagaagacg ctggtcgggg attttctcac cttccgggga
     2821 gatcagcacc ccgtcctccc cccatgtcca cccggcgtct gcgaggcgct tcttcgccgc
     2881 gtcggggtcg aacggatacg ccgcgacgtt cggctcgtag tacggggaga ccgggggaag
     2941 aaacgtcgag agcggctccg cgtaccccgc gaaaagatcg cgcacgacgc gctccctcgg
     3001 aagggcctgc cacgccgcct gccgcagttc gagccggttc cacgggaagg tgcggacgtt
     3061 gaacccgagg aaaaagccgt gaaagccgga tgcgagcgag aggttcaccc tcggatcgtc
     3121 ggcgagcgta tcgacgtcga cggggcggtg cagatctccg aggatatccg cctgccctcg
     3181 ctgaagcgcc agaagctggg cgtcgggatc cctgatgatc accaggtaaa gatcccggat
     3241 tttcggagag gagtagctct ccgcgttgaa ctttccgttt gcgctccatg cgcttccgga
     3301 aagaaaacag acgattgtaa ggacaagtgc gaaggcgatc ggtctcttca tctctttccc
     3361 atccttcccg tatccctgag gagtatgttt tcaagtattt taccagaaga cgcggcgaaa
     3421 caacgtctga aaaaaagtgc cggaaccaga agagggagtc ccccggtacc ggcgctttct
     3481 gatttccgcg aatctgacga acgccctaaa gcggcttttt ccccatgatg atcgcgctgg
     3541 ccaacgagcc gttgatctcg tttcgttctt cgccggagag ttcggggaag gccatcgccg
     3601 ccattgcgtc ggggaagg
//
LOCUS       DAOPKU010000302         3611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_28626_length_3611_cov_4.784871, whole genome shotgun sequence.
ACCESSION   DAOPKU010000302 DAOPKU010000000
VERSION     DAOPKU010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28626_length_3611_cov_4.784871"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..2196
                     /locus_tag="PK849_09630"
     CDS             169..2196
                     /locus_tag="PK849_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPE66424.1"
                     /translation="MGIASDLILVVLAGLVGGLIARRLNQPLILGYILAGIVVGPYTG
                     GITVADVDRIENLAEIGVALLLFSLGLEFSLKQILPIKRIALGGTALQALLTIALGFA
                     IGHFMGWAPLASLWFAVGIISSSIAVILKTLSSRGQLGTLSSRVMLGMSIVQDIAVIP
                     LMVLLMGLDDAVFSVLSIFVPVVKVVLFVSAMLYIGAKFIPLLLKHVARWESRELFLL
                     TVTGIGLGIGYLTYALGLSFAFGAFMAGLVLSESDYGRRALSDLIPVRDLFGLLFFVA
                     IGMLLDPAFLLDHIGVTLGLVTTVAVGRGVILSATVRLFGFRNVIPAAVFFGMIPISE
                     IAFILLQAGLDSGAVPWDVYSFALNTVILSMLLGPPATALTAPVYALFKRRRKVDAVQ
                     TVNISPDGLSGHVIVAGGGIFARHIGLVLHSMRLPYVVIEPNHALFLEAKKKGLRLLL
                     GDPGQDAVLEAACVEKAKLLVLTLHGALEIQDVVSAVRKRNRTLRILARDVSGEGGEF
                     PDEDREISRIIQPEFEAGLEMARQALIHLDVPGATIHRELETLRRNVYGPLRENNADY
                     GLLSQLLHAASTLELEWEAIHKDGPLEGKTIAAANIRAEFGVSVVAVLRDGALLPNPG
                     GDFILREGDLVGIIGTMEQNQKFFCFLDEQNERCRCASAEPPKTEKGENEK"
     gene            2193..3242
                     /locus_tag="PK849_09635"
     CDS             2193..3242
                     /locus_tag="PK849_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPE66425.1"
                     /translation="MTSSQRIEDYGIRIGRMPKGLRNTIADVPGVAVGHCTLADGPVQ
                     TGVTAVIPAPGNLFREKVMAACHVINGFGKSCGIVQIEEMGTLETPILLTNTFGVGAA
                     SEALVRSMLAANPDIGRETGTVNPAVFECNDGFLNDIRALPVRGEHVLRAIASAGADF
                     EEGAVGAGRGMSCYQLKGGIGSASRIVECGGASFTLGALVLSNFGQMEDLTVDGRKVG
                     ASIFGIAPPEDEQGSIIALLATDVPLTERQLKRVARRASVGITRTGAFIGNGSGEIAV
                     AFTTANRVFHDETAPVRQIGMFNENEINLLFRAAVEATEEAVLNSMVRAEAVTGRDGH
                     TRRSLAEFAELFAER"
     gene            3434..>3611
                     /locus_tag="PK849_09640"
     CDS             3434..>3611
                     /locus_tag="PK849_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE66426.1"
                     /translation="MRVLDKESPVPLYYQLKEILQAMVDDGELAPGDIVPSERDICER
                     FSISRMTASKAVSAL"
BASE COUNT          655 a         1168 c         1088 g          700 t
ORIGIN      
        1 ccgcacgtct cttttatttg cttattagta tatgggaagc catttaaaag tcaaggattt
       61 ttattgtctt tttttatacc gcctaccagt gggcgcaaga aatgctttgc actttttctc
      121 cgaggatgca agaataggag caagataaac cggaaagaag gagatcgcat gggcatcgca
      181 tcggatctta ttctcgtcgt tctcgcgggg ctcgtcggcg gactcatcgc cagacggctg
      241 aatcagccgc tcatcctcgg gtatattctg gcgggcatcg tcgtcggccc ctacactgga
      301 ggaatcaccg tcgcggacgt cgaccgcatc gaaaatcttg ccgaaatcgg cgtcgcgctc
      361 cttcttttct ccctggggct cgaattctcc ctcaagcaga tcctcccgat caagcggatc
      421 gccctcggtg ggaccgctct ccaggcgttg ctgacgatcg cgctcgggtt cgccatcggg
      481 catttcatgg ggtgggcccc cctcgcgtcg ctctggttcg cggtagggat aatctcgtcg
      541 agcatcgcgg tcatcctcaa gacgctctcc tcgagaggac agctcggcac gctgtcgagc
      601 agggtgatgc tgggcatgtc catcgtgcag gacatcgcgg tgattccgct gatggtgctt
      661 ctcatggggc tggacgacgc agtcttttcc gtcctctcca tcttcgtccc cgtcgtcaag
      721 gtcgtgctct tcgtctccgc gatgctctac atcggcgcga agttcatccc gctcctcctg
      781 aagcatgtgg cgcggtggga gtcccgggaa ctctttcttc tcacggtcac cggcatcggc
      841 ctcgggatcg ggtacctgac ctacgcgctc gggctctcct tcgccttcgg cgccttcatg
      901 gcaggccttg tgctcagcga atcggactac ggccgccgcg cattgagcga ccttattcct
      961 gtccgcgacc tcttcggact gctcttcttc gtcgccatcg gcatgctgct cgaccccgcc
     1021 tttctgctgg accatatcgg cgtcaccctc ggactcgtga ctaccgtcgc cgtaggccga
     1081 ggcgtcatcc tttcggccac ggtgaggctg ttcggcttcc gcaacgtcat ccccgccgca
     1141 gtcttcttcg gcatgatccc gatctccgaa atcgcgttca tcctgctgca ggcaggattg
     1201 gactccggcg cggttccgtg ggacgtctat tccttcgccc tgaacacggt catcctctcc
     1261 atgcttctgg gtccgcccgc cactgcgctg accgcgcctg tgtacgcgct cttcaaaagg
     1321 cggcgaaaag tagacgcggt ccagacggtc aacatctctc ccgacgggtt gtccgggcac
     1381 gtgatcgttg ccggcggagg catcttcgcc cgacacatcg ggctcgtcct ccacagcatg
     1441 cggctaccct acgtcgtcat cgagccgaac cacgccctct ttcttgaggc aaaaaagaaa
     1501 ggactgcgac ttcttctcgg agatcccgga caggacgcgg ttctggaagc ggcctgcgtc
     1561 gagaaggcaa agctccttgt cctgacgctc catggggctc tggagattca ggacgtagtc
     1621 agcgcggtcc ggaagcgcaa tcgcactctc cggattctgg cccgcgacgt cagcggcgag
     1681 ggcggggagt tccccgacga ggaccgcgaa atttcgcgca tcatccagcc cgagttcgaa
     1741 gcggggcttg agatggcccg gcaggcgctc atccaccttg acgtaccggg cgcgacgatc
     1801 cacagggagc tggagacgct ccggcggaac gtctacggcc cgctgcggga gaataacgcc
     1861 gactacggcc ttctctcgca gctgctgcac gccgcctcga cgctggaact ggagtgggag
     1921 gccatacaca aggacggacc gctcgaggga aagaccatcg ccgcggcgaa catcagggcc
     1981 gagttcggcg tctcggtggt ggcggtgctc cgcgacggcg cgcttctgcc caaccccgga
     2041 ggcgacttca tactgcggga aggagatctc gtcggcatca tcggcacgat ggagcagaac
     2101 cagaaatttt tctgcttcct cgacgagcag aacgaacgat gccgctgcgc atcagcagaa
     2161 cctccgaaaa cagaaaaagg agagaacgaa aaatgacatc ttcacaacgc atcgaggact
     2221 acggcatccg catcggccgt atgcccaaag gactgcggaa cacgatcgcc gacgtccccg
     2281 gcgtcgccgt cggacactgc accctcgccg acggcccggt acagaccggt gtgaccgccg
     2341 ttattccggc gccggggaac ctcttccgcg aaaaagtcat ggcggcgtgc cacgtgatca
     2401 acggtttcgg caagtcgtgc gggatcgtcc agatcgagga gatgggcacg ctcgagacgc
     2461 cgatcctgct gaccaacacc ttcggcgtgg gcgcggcgtc cgaggcgctc gtccgctcta
     2521 tgctcgccgc gaaccccgac atcggcaggg agaccgggac ggtgaacccg gcggtcttcg
     2581 agtgcaacga cggcttcctg aacgacatcc gcgcgcttcc ggtccgcggg gagcacgtgc
     2641 tgcgggccat cgcctcggcg ggcgccgact tcgaggaggg cgcggtcggc gcggggcgcg
     2701 gaatgtcgtg ctaccagctc aaagggggaa tcggctcggc gtcgcgcatc gtcgagtgcg
     2761 gcggcgcgtc gttcacgctg ggcgcgctcg tgctctccaa cttcggccag atggaagacc
     2821 tgaccgtgga cggacggaag gtcggcgcct ccatcttcgg gatcgcccct ccggaggacg
     2881 agcagggatc gatcatcgcg ctgctcgcga ccgacgtccc tctcacggag cggcagctca
     2941 aaagagtcgc ccggcgcgcc tcggtcggca tcacgcgcac cggcgccttc atcggcaacg
     3001 ggagcggcga gatcgccgtc gccttcacga ccgccaaccg cgtcttccac gacgaaacag
     3061 ctcctgtgcg gcaaatcggc atgttcaacg aaaacgagat caacctcctc ttccgcgccg
     3121 ccgtggaagc gaccgaggag gccgtgctca actcgatggt ccgagccgag gccgtcacgg
     3181 ggagagacgg acacaccagg cgctcactcg ccgagtttgc cgagctgttt gcggaacgat
     3241 aaccacatcg ccttgcgagc ggaccccgaa ggctagagaa aacacaaacg cgggcttata
     3301 ggaggaactc gcattggcct ccctcttcca agagtcttgt tttcttttga aacttcgctt
     3361 gacgcactcg ccgccgacgt ggtatagtcc acataacatg catacgactc tgccgctcgg
     3421 ggggaactgt ttcatgcgcg tactggacaa agagagcccg gttccgcttt attaccagct
     3481 caaggagatt ctgcaggcga tggtcgacga cggggagctt gcccccggcg acatcgtccc
     3541 ttccgagcgc gacatttgcg agcggttctc catcagccgc atgacggcga gcaaggccgt
     3601 ctccgcgctg g
//
LOCUS       DAOPKU010000303         3591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_28870_length_3591_cov_4.292704, whole genome shotgun sequence.
ACCESSION   DAOPKU010000303 DAOPKU010000000
VERSION     DAOPKU010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28870_length_3591_cov_4.292704"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK849_09645"
     CDS             <1..388
                     /locus_tag="PK849_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPE66427.1"
                     /translation="ASIPGLRLRAVALSRAFVAGGFAAFTWLRNDDFLHNGSERSATE
                     FLVNDLLTLKDVSFAAFFVEDEECVRVSLRSRGVVQAAEIARVFGGGGHPQAAGCKLP
                     LPLSDAVESVRSLVEERYAERIAPAE"
     gene            360..1274
                     /gene="truB"
                     /locus_tag="PK849_09650"
     CDS             360..1274
                     /gene="truB"
                     /locus_tag="PK849_09650"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPE66428.1"
                     /translation="MPSGLLLLNKPAGVRSAVCVAIAKRRLNREFKIGHGGTLDSTAE
                     GLLVLLIGGATRTSDLVMSLPKLYEAAFTLGEERSTDDYSGDTVFEGRVPNDARARIE
                     AALPSFLGVRLQLPPGISAVRVGGRRAHAVVRSGEELSLRSRPVFVRSISLLPGEEMK
                     NVFTIRVLCGKGTYIRSIVRDIGRMLGCGAYVLRLIRKQSGLLSLGQALPFEEFQNGD
                     ADLRSHIFPLARFAENFYCYEADEERTARLRDGLAVPLSSLRFSHEGILSPDQGAAVL
                     GKTLFCYGRVLKEGFFKPKINIDLEGSE"
     gene            1271..2212
                     /gene="ribF"
                     /locus_tag="PK849_09655"
     CDS             1271..2212
                     /gene="ribF"
                     /locus_tag="PK849_09655"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HPE66429.1"
                     /translation="MIVVLGAFDGFHRGHALLLDRARELSGRHSRSAANGEWAVVTFS
                     PHPDSVVRGGMFSLFSEDERDLLVRFFNVPHIEKIPFDETLSRTSAEGFIALLESLLP
                     VTGVIVGEDFRFGRDREGDAAYLREVARRKGWLFDKLDSFVLEGAKVGSSSIKKHVIS
                     GDVDRARNLLGYPFFFRGRVVSGDGRGRTLGFPTANIEYPEKKILPQGGVYAASVLIG
                     NEWWPGALNIGFNPTFLSSVENPRFETYVLDLNESLYGRTITVFLESLLRPEIKFESS
                     DALLHQMQLDVLRVRDCFREMHARHGDVYCHLSKAFS"
     gene            2324..2399
                     /locus_tag="PK849_09660"
     tRNA            2324..2399
                     /locus_tag="PK849_09660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2357..2359,aa:Arg,seq:cct)
     gene            2433..2939
                     /locus_tag="PK849_09665"
     CDS             2433..2939
                     /locus_tag="PK849_09665"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HPE66430.1"
                     /translation="MLDAIRKRAFSLLLFFSLIFLLCVPVESLTVRSSDGSSISVCPV
                     APGDSFMLSFIHSVEKTPVESEFRVLSGKVRQWEERFLSHNAGLPTEAPPNGRFIMDK
                     DWMILRGGGLAVQSIRYRVGNEQFGRNYMVLPDGAKILLFEEYPGRILFFSAESTSFL
                     NYLFSQVF"
     gene            2962..>3591
                     /locus_tag="PK849_09670"
     CDS             2962..>3591
                     /locus_tag="PK849_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HPE66431.1"
                     /translation="MAEVPEKDGVNGQNEIDLDALRRQFDTEARYRKLSGFPAILVTS
                     LAVCMSLFHLYTSGFGLMQAIIQAAVHLAFVLVLVFLLYPFSPKFSKTEIPWYDYLLA
                     ALGAFVTLYLVFEFDALITRAGLPTTMDLIVAFLGVAILLEATRRVTSPILPVIAIFF
                     LLYCYYGRYFPALFQHRGFNINRIVNHMYLGTEGIFGIPLGVSATFVFMF"
BASE COUNT          746 a          883 c          960 g         1002 t
ORIGIN      
        1 tgcatccatt cccggccttc gtttaagggc ggtggcattg tccagagcct tcgttgcagg
       61 cggttttgcg gcgtttacat ggttgcggaa cgatgatttt ctccataacg gcagcgaacg
      121 ctctgcaact gagttcctgg taaacgatct cctcacgctc aaggatgttt cgtttgccgc
      181 tttctttgtg gaggacgagg agtgcgtccg cgtatcgctc cggtcgagag gcgtcgttca
      241 ggcggctgaa atagcgcgcg tattcggtgg aggagggcat ccccaggccg cggggtgcaa
      301 gcttcctctt ccattatcgg acgctgtgga gagtgtacgc tctctggtgg aggaacggta
      361 tgccgagcgg attgctcctg ctgaataaac ctgccggcgt acgaagcgcc gtctgtgtag
      421 ccatcgcgaa aaggcgcctg aacagagagt tcaagatagg tcacggcggc acccttgatt
      481 ctacagctga aggcctgctt gtgctcctga ttggaggagc tacccggacg agcgatcttg
      541 tgatgtccct gccaaaactc tacgaggccg ccttcacgct tggagaggag cgctcaaccg
      601 acgactactc cggcgatact gttttcgaag ggcgcgtccc gaatgatgca cgggctcgta
      661 tagaggcggc gctcccgtca tttctcggag ttcggctcca acttcctccg ggaatatcag
      721 ccgttcgagt aggaggaaga cgggctcatg ctgtcgtgcg ttcgggagaa gagctctctc
      781 ttcgcagccg acctgttttt gtacgttcca tttcgctcct tcccggcgaa gagatgaaaa
      841 atgtgttcac aatacgcgtt ctctgcggga aaggaacata tattcgcagt atcgttcgcg
      901 atataggaag aatgctcggg tgcggggcat acgtcctccg gcttatccga aaacagtccg
      961 gattactctc tttggggcag gctctccctt ttgaagagtt ccaaaacggg gatgccgatc
     1021 ttcgatcaca cattttcccg ctcgctcgtt ttgcggagaa tttttactgc tatgaagccg
     1081 atgaagaacg aacagcccgt ctgcgcgacg gcctcgcagt gccgctttcc tcccttcgtt
     1141 tttcgcatga aggtatactt tctcctgacc agggagcggc ggtcctggga aaaactcttt
     1201 tttgctacgg acgagttctc aaggaaggat tcttcaagcc gaaaatcaat attgatctgg
     1261 agggatccga atgattgtcg tgcttggagc tttcgacgga ttccacagag gacacgctct
     1321 tcttctcgat agagccagag agctttccgg gcgacatagc cgttctgctg caaacggaga
     1381 gtgggcagta gttacttttt ctccgcaccc ggattcggtt gtccgggggg gaatgttttc
     1441 cctcttttcc gaggatgagc gcgatcttct cgttcgcttc ttcaacgttc cgcatattga
     1501 aaaaattcca tttgacgaga cgctctcacg gacctcggcg gagggattca ttgccctgtt
     1561 ggagtcgctt cttcctgtga ctggagttat agtgggagaa gattttcgct tcgggcgcga
     1621 ccgggagggg gacgcggcat acttacggga ggtggccaga cggaagggat ggctctttga
     1681 caagttggat tcctttgttc ttgaaggagc aaaagtcggt agcagcagta taaaaaagca
     1741 tgtcatttca ggagacgtcg atcgtgccag aaatctgctt ggatatcctt tctttttcag
     1801 gggaagggtt gtctctggag atggcagagg gcgtacttta ggcttcccta ccgcaaacat
     1861 cgaatatccc gagaaaaaaa ttctgcccca aggaggagtc tacgccgcct cggtactgat
     1921 cggaaacgaa tggtggccgg gagccctgaa tattggtttt aacccgacat ttctttcctc
     1981 cgtagaaaac cctcgtttcg aaacctatgt gctggacctg aacgaatccc tctacgggag
     2041 aaccataacc gtcttccttg agtcgcttct gcgtcccgaa atcaaatttg aatccagcga
     2101 cgctcttctt catcagatgc agctcgatgt tcttcgagtg agagactgct tcagggaaat
     2161 gcatgcccgt cacggtgatg tctattgcca tttgtcaaaa gcattttctt gacgtgtgcg
     2221 ccttgcgact tgaaaaatcg gggagagggt ggtactattc ctcgtgctgg cgattttccg
     2281 gcatcagtca tttgagattt ccagaaataa atgttatgaa gacgggcctg tagctcagag
     2341 gatagagcgg caccctccta aggtgtaggt cggccgttcg aatcggccca ggctcgccat
     2401 ttttttgttt ttgggagaaa gaggtgtaca atgtgcttga tgccattcgt aaacgtgctt
     2461 tctcgctttt gctctttttt tctcttattt ttttattgtg cgtgccagtt gagtcattaa
     2521 cagttcgctc ttccgacggg agttcaattt ctgtttgtcc cgtcgcccct ggagattctt
     2581 ttatgctgag tttcattcat tcggttgaaa agactcctgt ggagagtgag ttccgagttc
     2641 tttcaggcaa ggtgcgtcag tgggaagaac gttttctttc ccataacgca gggttgccca
     2701 cggaagctcc tccaaacgga cgattcatca tggacaagga ttggatgatt ctgagaggag
     2761 gaggacttgc cgtccagagt atccggtata gggtcggcaa cgagcaattc ggcaggaatt
     2821 atatggtcct gccggacgga gcaaagatac ttctttttga agaatacccg ggccgcattc
     2881 ttttcttttc agctgaatca acgtctttct tgaattatct tttttcacaa gtgttttgaa
     2941 tacgatcagg aggtggtttt attggccgaa gttcccgaga aggacggagt gaacggacag
     3001 aacgaaattg atcttgacgc tcttcggcga cagttcgaca cagaggccag gtacaggaag
     3061 ctctcaggct ttcctgccat tcttgttact tctctggcgg tatgtatgtc gctgtttcat
     3121 ttgtatacat caggttttgg tcttatgcag gctattatcc aggcggccgt ccacttggca
     3181 tttgtcctcg tccttgtctt cctgctctat cctttttcgc caaagttttc taaaaccgaa
     3241 attccctggt atgactactt gttagccgcc ctaggggcat tcgtcacact gtatctggtt
     3301 tttgagttcg atgctcttat tacccgggcg ggcttgccga cgacaatgga tcttatcgtt
     3361 gcattcttgg gcgtcgctat tcttctggaa gcaaccagac gagttacgag cccgattttg
     3421 cccgtcatcg ccatcttctt cctcctgtat tgctactacg gacgatattt ccccgcgctc
     3481 ttccaacaca ggggattcaa catcaaccgt atagtgaacc atatgtacct cggaactgaa
     3541 ggaattttcg gtattcccct cggtgtgtcc gcgacatttg tgttcatgtt c
//
LOCUS       DAOPKU010000304         3551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29309_length_3551_cov_4.787471, whole genome shotgun sequence.
ACCESSION   DAOPKU010000304 DAOPKU010000000
VERSION     DAOPKU010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29309_length_3551_cov_4.787471"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PK849_09675"
     CDS             <1..380
                     /locus_tag="PK849_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE66432.1"
                     /translation="STISQYEIDAYLSDYDIEEHIKKLISVYRERRDLMIRTMDASFP
                     KEVVFTRPEGGLFTWAEMPEHVNARDLFLKALEKKVAFVPGGAFFPNGGHENTMRLNY
                     SNMPPERIVEGIERLGAAIREYL"
     gene            513..1427
                     /gene="ftcD"
                     /locus_tag="PK849_09680"
     CDS             513..1427
                     /gene="ftcD"
                     /locus_tag="PK849_09680"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPE66433.1"
                     /translation="MAGKLIECVPNFSEGRRQDVIEAIVAPFRATKGCGLLDYRADAD
                     HNRLVVSLAGSPEPVQEALLKAAAVAVQKIDMNSHQGAHPRIGAVDVIPFTPVSNISM
                     EECVALSHSFGERYWKELGVPVYFYEDSAKRPERKRLEVIRKGQYETLKTESVTPERA
                     PDVGGPALHPTAGATVMGARKFLVAFNVNLNTADEEIAKKIANCVRASSGGFCHVKGM
                     GVALHERGMAQVSMNIVDFEKNAVYRVLELIRMEARRWGVEVVETEIYGMIPAQALVE
                     SAAYYLQIKDFDPMQVLELKLLSGGDAE"
     gene            1424..2665
                     /gene="hutI"
                     /locus_tag="PK849_09685"
     CDS             1424..2665
                     /gene="hutI"
                     /locus_tag="PK849_09685"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPE66434.1"
                     /translation="MIVKFFRNARIYTPEGKPPSRGREQGALRVHERGAMLVQDGVLE
                     AVGTEEELLSNPLVKKVDEEIDCEGRCLIPGFVDPHTHMCFAARREEEFSMRLAGAAY
                     LDILKKGGGILSSVRHVRGASEQEIFERTRTLALSALSFGTTTVEMKSGYGLDTESEL
                     KMLSAIRRVGRETPLRVVPTFMGAHAIPEVFKGNGDGYVDLMIGEMLPAVAEQGIARF
                     CDVFCEEGVFSPEQTRKVLVEARKYGLGLKIHADEVHDTGGAALAAELGAVSAEHLLA
                     ASDEGIEALAASGTVAVLLPATAYSLRKPFARARTMIEKGVPVALATDCNPGSSFTES
                     MPFVFGLAVLVMGLTVEEALVATTKNSAHAIGLGAECGTLEPGKNADFLLLDGETPAV
                     LAYHAGVSPVKSVFKKGERVA"
     gene            2665..>3551
                     /locus_tag="PK849_09690"
     CDS             2665..>3551
                     /locus_tag="PK849_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HPE66435.1"
                     /translation="MIGERKNRVRLDGMSLTLEDVLRVARDGWGVELDEEAARKVEEA
                     SAAVKAWENSDDVVYGITTGFGDLATVNISRKDRRLLQENLLKSHACGVGEPFSEEIT
                     RAMMLLRINSLIRGFSGISLATLHQFVAFLNLGITPVVPSQGSVGASGDLCPLSHLAL
                     PLLGLGKVRYKGRVILAAKALQTAGLQPVQLGAKEGLALNNGTASMGAMGVVALMAAE
                     DLAKTADIAAALSIEALHGVPYAFDARTHALRAHRGQGRVAENIRRLIEGSEIIEKHR
                     FARVQDAYSLRCVPQVHGAT"
BASE COUNT          681 a         1082 c         1168 g          620 t
ORIGIN      
        1 cgtcgacgat cagccagtac gagatcgacg cctacctctc cgactacgat attgaggaac
       61 acatcaagaa actgatctcc gtataccgcg agcggcgcga cctgatgatc cgaaccatgg
      121 acgcatcatt cccgaaggag gttgtgttca cgcgtcccga gggagggctc ttcacatggg
      181 ccgagatgcc ggagcatgtg aacgcgcggg acctcttcct caaggcgctc gagaagaagg
      241 ttgccttcgt ccccggagga gcgttcttcc ccaacggggg gcacgagaac acaatgcggc
      301 tcaactactc caacatgccg ccggagcgca tcgtcgaagg gatcgaacgc ctgggagcgg
      361 cgatccgcga gtatctctaa aaaaacgaat gagagaggca gggctgaaac aaggggtcct
      421 gcctctcttt ttactttcgc gctttacgtt gtacatgttt caatgttatc atgtaccgtg
      481 cagcatacta atacccgcag gaggttgttc ccatggcagg caagctcatc gagtgtgttc
      541 cgaatttcag cgaaggacgg cgtcaggatg tgatcgaggc gatcgtcgct ccgttcaggg
      601 cgacgaaggg gtgcggtctt ctcgattatc gggcggacgc ggaccataac aggctggtcg
      661 tgagcctcgc gggatcgccc gagcctgttc aggaggcgct tctcaaggcc gccgccgtcg
      721 cggtgcagaa gattgacatg aacagccacc agggggcgca tccgcgaatc ggcgcggtgg
      781 acgtgattcc tttcaccccc gtgagcaaca tttctatgga ggagtgcgtg gcgctctcgc
      841 attcgttcgg cgagagatat tggaaagaac tcggcgttcc ggtctacttc tacgaggatt
      901 ccgcgaaacg cccggaacgg aagcgccttg aagttatccg caaggggcag tacgagacgc
      961 tcaagaccga gtccgtcaca ccggagcgcg ctcccgacgt cggcggtccc gccctgcacc
     1021 cgacggcggg cgcgacggtg atgggcgccc ggaagttcct cgtggccttc aacgtcaacc
     1081 tgaacaccgc ggacgaggag atcgcgaaga agatcgccaa ctgcgtccgc gcctccagcg
     1141 gcggcttctg ccatgtcaag ggcatgggcg tggcgctcca cgagcgcggc atggcgcagg
     1201 tgagcatgaa tatcgtcgac ttcgagaaga acgcggtgta cagggtgctc gaactgatcc
     1261 ggatggaggc cagacgatgg ggcgtcgagg tcgtcgagac ggagatctac ggaatgattc
     1321 ctgcgcaggc tcttgtggag agcgcagcgt actatttgca gatcaaggac ttcgacccga
     1381 tgcaggttct ggagctgaag ctcctctccg gcggtgacgc tgaatgatcg tcaaattctt
     1441 ccgcaacgcc cgcatctaca ctccggaggg aaagcctccc tccagggggc gtgagcaagg
     1501 ggctctgcgc gttcatgaac gaggggcgat gctcgttcaa gatggagttc tcgaagccgt
     1561 cggcacggaa gaggagcttc ttagcaatcc cctcgtgaaa aaggtcgacg aagagatcga
     1621 ctgcgaaggg cgctgtctga tccccggctt cgtcgatccg cacacgcaca tgtgcttcgc
     1681 cgcgcggcgg gaagaggagt tctcaatgcg cctggcgggg gcggcgtacc tcgacatact
     1741 caagaagggc ggcggcattc tctcctccgt ccggcatgta cggggcgcga gcgaacagga
     1801 gatcttcgag cgcacgagga cgctcgcgct ctccgcgctc tccttcggga cgaccactgt
     1861 cgagatgaag agcgggtatg gactcgacac ggagagcgag ctgaaaatgc tttcggcgat
     1921 ccgccgcgtc ggacgcgaaa cgcccttgcg cgtcgttccc acctttatgg gggcgcacgc
     1981 gattccggag gtcttcaagg ggaacggcga cggctacgtc gacctcatga tcggagagat
     2041 gcttcccgcc gtcgcggaac agggaatcgc ccgcttctgc gacgtcttct gcgaggaggg
     2101 ggtcttctcc ccggagcaga cgcggaaagt gctcgtcgag gcccgaaaat acgggctcgg
     2161 cctgaagatt cacgccgacg aggtgcacga caccggcggg gccgcgcttg ccgccgaact
     2221 cggcgccgtt tccgcggagc atcttctcgc cgcgagcgac gaagggatag aagccctcgc
     2281 cgcgagcgga acggtcgccg tactgctccc cgccacggcg tacagcttgc gcaagccgtt
     2341 cgcacgcgcc cggaccatga tcgagaaggg ggttcccgtg gcccttgcga ccgactgcaa
     2401 ccctggttcc tccttcacgg agtccatgcc cttcgtcttc ggcctggccg tactcgtgat
     2461 gggactcact gtggaagagg cgcttgtggc gacgacgaag aacagcgcgc acgcgatcgg
     2521 cctcggggcc gagtgcggga cgctcgagcc gggaaagaac gccgacttcc tcctcctcga
     2581 cggagagact ccggccgtcc tggcctacca cgccggggtc tcgccggtga agtccgtctt
     2641 caagaaaggg gagcgtgtcg catgatgatc ggtgagcgga agaacagagt acgtctcgac
     2701 gggatgtcgc ttaccctcga ggatgtgctc cgcgtcgcgc gcgacgggtg gggagtcgaa
     2761 ctggacgaag aggccgcccg gaaggtggag gaggcgtccg ccgccgttaa agcctgggag
     2821 aactccgacg acgtcgtcta cggcatcacc acgggcttcg gcgacctcgc cacggtgaac
     2881 atctcccgca aggatcgtcg cctgctgcag gagaatctct tgaagagcca tgcctgcggc
     2941 gtcggcgagc cgttctccga ggagatcacg cgggccatga tgctgctgcg gatcaacagt
     3001 ctcatcaggg ggttttcggg tatcagcctc gcgacgctgc atcagttcgt cgcatttctg
     3061 aacctgggaa tcaccccggt cgtcccgtcg caggggtcgg tgggcgccag cggcgatctt
     3121 tgccctctct cgcacctcgc gctcccgctg ctggggctcg gaaaggtgcg ttacaagggg
     3181 cgcgtcatcc tcgcggccaa agcgctccag acggcggggc tccagcctgt gcagctcggg
     3241 gcgaaggagg gactcgccct gaacaacggc acggcctcga tgggcgccat gggcgtagtg
     3301 gcgctgatgg ccgccgagga ccttgccaag accgcggaca tcgcggcggc gctctccatt
     3361 gaagcgcttc acggcgtccc ctacgccttc gacgcgcgga cgcacgcgct ccgcgcccac
     3421 agagggcagg ggcgcgtggc cgagaatatc cgccgactga tcgaggggag cgagatcatt
     3481 gagaaacatc gcttcgccag ggtgcaggac gcctactccc tccgctgcgt gccgcaggtt
     3541 cacggcgcca c
//
LOCUS       DAOPKU010000305         3549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29331_length_3549_cov_5.153692, whole genome shotgun sequence.
ACCESSION   DAOPKU010000305 DAOPKU010000000
VERSION     DAOPKU010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29331_length_3549_cov_5.153692"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PK849_09695"
     CDS             complement(<1..881)
                     /locus_tag="PK849_09695"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPE66436.1"
                     /translation="MPGSELFDHLEIEAVADVLKRKVIHRYAYHEVRDGNYRVDEFEA
                     KVAELSGAKHCLAVSSGTAALYVPFKAMGICPGDEIITSSFTFIATVEAILECGAVPV
                     LGDVDESLNLSPDSAEDLITERTKAIMPVHMFGAAADMDPFRALGAKYGIPVVEDACQ
                     AMGGTYKGKALGTMGLWGSYSLDPNKLLTVGEGGLIVTDDDEIYSKMEYYHDHGHIHS
                     KILDRGAEGKACLGFNFRMSELQGALGLVQAAKMQQAISMTRKTKSTILDAVKKTGLE
                     LRDLPDREGEIATQIVFL"
     gene            complement(883..2040)
                     /locus_tag="PK849_09700"
     CDS             complement(883..2040)
                     /locus_tag="PK849_09700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPE66437.1"
                     /translation="MQFNMFLAGRIAFGPGVSSLTGEKASALGGKRIFLVTDRTMEKL
                     GTAEKIVSYINAAGLSVIPFYDVEPEPSVETVDAATALAKREKCDLVVGLGGGSCLDV
                     AKATSVLLTNEGSAARYQGLGLVKNAGVPKIMIPTTAGTGSEVTFTSVLIRKSDGVKG
                     GINGDQLYPECSLLDPELTVSMPSSVTAATGMDALAHALEAYTSKQASPFSDMFAEQA
                     ISRIGRYLRVAVYNGTDIHARSEMMLAALYGGIALANAGVTACHALAYPLGSMFGIGH
                     GQANALLLPYVAQFNAMALPEKFRAAAELLGGSYEEESLWDAASMCSALLHQLVADIN
                     LPSTFSELDPRITEDHFATMADKAMGVARPMANNPRTMNTDLCMEIYKEAM"
     gene            complement(2103..2816)
                     /gene="kdsB"
                     /locus_tag="PK849_09705"
     CDS             complement(2103..2816)
                     /gene="kdsB"
                     /locus_tag="PK849_09705"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPE66438.1"
                     /translation="MNALAVIPARYASTRLPGKPLLPVAGVPLVLRVLEQVQKCSGVD
                     RIIVATDDQRIADVVENAGAEALLTPPELPSGGDRVAWVSERIPSKIVLNIQVDDPLV
                     GPDMIDPLIAALSDEGTALALLAKRIENEREIESPNIVKMVFDKNQRALYFSRSPIPY
                     PRTSGAAYYKHIGPYAWKREFLLQFSGWNQTPLEKSESLEMLRVLENGYSIRCIPVAR
                     DTIEIDTPEDVAAFEALLA"
     gene            complement(2951..>3549)
                     /locus_tag="PK849_09710"
     CDS             complement(2951..>3549)
                     /locus_tag="PK849_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPE66439.1"
                     /translation="VDPADPAFTHPTKPVGPFYTEEAAKKRIAETGETWIDDAGRGWR
                     RVVPSPDPKSIVEADVINTLLEKGFTVVASGGGGIPVIRKENGTWEGVEAVIDKDLAG
                     ERLAASVKADLLMILTDVPKVAIHYNTPQQKWLDKLTLSEMESLEKEGHFKAGSMGPK
                     VKAALRFVRNGGQRAVIASLDEALEALEGTKGTQILKG"
BASE COUNT          687 a         1009 c         1018 g          835 t
ORIGIN      
        1 aggaagacaa tctgcgtggc tatttcgcct tcacggtccg gaagatcacg gagttcgagc
       61 cccgtctttt tcacggcgtc cagaatcgtc gatttcgtct ttctggtcat cgagattgcc
      121 tgctgcattt ttgcagcctg aacgaggcca agggcgcctt gcaattcgct catgcggaag
      181 ttgaatccaa ggcaagcctt tccttcggcc cctctgtcca ggattttgct gtggatgtgc
      241 ccgtgatcgt ggtagtactc cattttgctg tagatctcgt cgtcgtcggt cacgatcaat
      301 ccgccttcgc cgacagtgag gagtttgttc gggtccagac tgtaggaacc ccagagtccc
      361 atagtcccga gcgccttccc tttatacgtc cctcccattg cctggcaggc gtcctcgacg
      421 acggggatgc cgtatttcgc tcccagtgcc cggaacgggt ccatgtccgc cgcggcgccg
      481 aacatgtgca caggcatgat cgctttcgtg cgctccgtta tgaggtcctc cgccgaatcg
      541 gggctgaggt tcaggctttc gtcgacgtct ccgaggaccg gaacggcccc gcactccagg
      601 atggcttcaa ccgtggcgat aaaagtgaac gaggatgtga tgatctcgtc tccggggcag
      661 atccccatcg ccttgaacgg aacgtaaagc gcagccgtac cgctgctcac ggcgagacag
      721 tgcttcgctc cggagagttc cgcgactttt gcctcgaatt cgtccaccct gtagtttccg
      781 tcccgtacct catgataagc gtagcggtga atgactttgc gcttgaggac gtcggcgacg
      841 gcctctattt ctagatgatc gaagagttcg gaacccggca tgttacatcg cctccttata
      901 gatttccatg caaagatcgg tattcatcgt ccgggggttg tttgccatcg gacgcgcgac
      961 gcccatcgcc ttgtcggcca tcgtcgcgaa gtggtcttca gtgatacgag gatcgagttc
     1021 cgaaaaagtt gagggaaggt tgatgtcggc gacaagctgg tggagaagcg ccgaacacat
     1081 agaagccgca tcccagagac tctcctcttc gtaggaaccg cccaggagct ccgcggctgc
     1141 tctgaacttc tccggcagcg ccatcgcgtt gaactgcgcc acgtagggga gaaggagcgc
     1201 attcgcctgt ccgtgcccga tgccgaacat gctgccgaga ggataggcga gcgcgtggca
     1261 cgctgtgaca ccggcgttgg cgagcgctat gccgccgtac agcgcggcga gcatcatctc
     1321 gcttcgggcg tgtatatccg tcccgttata tacagcgacc cggaggtagc ggccgatacg
     1381 ggagatggcc tgctcggcaa acatgtcgct gaacggcgaa gcctgtttgc tcgtgtacgc
     1441 ttcgagggca tgggcgagcg cgtccatacc cgtggcggcg gtaacggagg acggcatgga
     1501 gacagtcagt tcggggtcaa ggaggctgca ctcagggtag agctggtctc cgttgatccc
     1561 tccctttacg ccgtcgctct tccgtatcag gacggaggtg aaagttactt ccgaccctgt
     1621 gccggcggtg gtggggatca tgatcttcgg aacgcctgcg ttcttgacaa gtccgagacc
     1681 ctggtagcgg gctgcggagc cttcgtttgt caggaggacg ctcgttgcct tcgccacgtc
     1741 gaggcagctt cctcctccga ggccgacgac gagatcgcac ttttctcttt ttgcaagcgc
     1801 ggttgcggcg tcgacggttt ctacggacgg ttccggctcc acgtcgtaaa aggggattac
     1861 ggacaaccca gcggcgttga tatatgaaac gatcttttcc gccgtcccaa gcttctccat
     1921 ggttctgtcg gtgacaagaa agattctttt gcccccaaga gcggacgcct tctctcccgt
     1981 gagggaagag actccggggc cgaacgcgat tcgtccggcc aaaaacatgt tgaattgcat
     2041 ctttccacct ctaatttcct tgcggctgtt gccgcggtct ctgtggttcg tcgatcattc
     2101 cgtcaggcga gcagcgcttc aaacgccgca acgtcctcag gagtgtcgat ttctatagtg
     2161 tcccgtgcaa cgggaataca gcggattgag tacccgtttt ccagaacccg cagcatctca
     2221 aggctttctg atttttcaag cggagtctgg ttccagccgg agaattgaag caaaaattct
     2281 cgtttccagg cgtacgggcc gatatgtttg tagtatgccg ctccggaggt gcgggggtag
     2341 gggatgggag acctgctgaa gtagagcgct ctctggttct tgtcgaagac catttttact
     2401 atgttcggcg attctatctc ccgttcgttc tctattcgct tggcaagcag tgcaagcgcc
     2461 gtgccttcgt ccgagagcgc cgcaatcagc gggtcgatca tgtccgggcc gacaagcgga
     2521 tcatcgacct ggatattcaa aacgatcttt gaaggaattc gttcggagac ccatgcgaca
     2581 cggtcgcctc cgcttggcag ctcaggcgga gtgagcagag cttccgctcc ggcgttttcg
     2641 acgacatcgg caattcgttg gtcgtcagtg gcaacgatga tgcggtcgac gccgctgcac
     2701 ttttgaacct gttccagaac tctgaggacg agcggtacgc ctgcgactgg aagaaggggc
     2761 ttccccggga gacgagtgct cgcgtaccgg gccggaatga ctgcaagagc gttcataaat
     2821 ccatctcctt gccggaaaaa aaccggaagt aaattctaca tcatttatta tcacctccat
     2881 agactctttt tcgcatcaac aaaaaacctc cctcttgagt gagggaggtt tatatcgcga
     2941 agataaaaaa ctaaccttta agaatctggg tacccttggt tccttcgagg gcttccagag
     3001 cttcatcgag gcttgcgatc accgcgcgct gtccgccgtt acggacgaac cggagtgcag
     3061 cttttacttt tggccccatg gaaccggcct tgaaatgccc ttctttttcg agagactcca
     3121 tctcggagag cgtcagcttg tcgagccact tctgctgcgg tgtattgtag tgaatggcta
     3181 ccttgggaac atcggtgaga atcatcagca gatccgcctt cacagaagcg gcaaggcgtt
     3241 cgcctgcaag atccttgtcg atgacggctt ccacaccctc ccatgttccg ttttccttcc
     3301 ggatgacggg aataccgccg ccgccggagg caactactgt gaatcctttt tcaaggagcg
     3361 tgttgatgac gtccgcttcg acgatcgact tgggatccgg ggaggggaca acccggcgcc
     3421 atcctcgtcc cgcatcgtcg atccatgtct ctccggtctc ggcaatgcgc ttcttagcgg
     3481 cctcttcagt atagaaggga cccaccggct ttgtgggatg ggtaaacgcc ggatctgcag
     3541 gatccactt
//
LOCUS       DAOPKU010000306         3549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29338_length_3549_cov_3.637092, whole genome shotgun sequence.
ACCESSION   DAOPKU010000306 DAOPKU010000000
VERSION     DAOPKU010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29338_length_3549_cov_3.637092"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PK849_09715"
     CDS             complement(<1..477)
                     /locus_tag="PK849_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPE66440.1"
                     /translation="MAGLDPNMVYGLVTGLLFGFLLQRGRVIRYDKQLGALRVQDFTI
                     LKFMLSSIVVAMVGVYILLDMGMVKLSVKPMIAGGVVVGGLLFGVGWGLLGYCPGTSV
                     AAVGEGRLDALWGIAGGLVGAALYAEAFPWMSRTLLTMGNYGKITIPQALGMNHWYV"
     gene            complement(458..991)
                     /locus_tag="PK849_09720"
     CDS             complement(458..991)
                     /locus_tag="PK849_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(599..601),aa:Sec)
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPE66441.1"
                     /translation="MKEEGGWNPYLAGGLSGLVSIGSVWFAGKYLGASTTFVRATAAV
                     EQAVLPERAATLEYLVKTAPKMDWQAWFLVGIFIGSLVAAALFGDFKFELVPPMWKKR
                     FGDGVLKRGVVAFIGGVVAMFGARLADGUPSGHGLSGSLQLAVSGIVAFAAFYIAGMV
                     TAKLVYGGGERNGGTGS"
     gene            complement(1135..1899)
                     /locus_tag="PK849_09725"
     CDS             complement(1135..1899)
                     /locus_tag="PK849_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPE66442.1"
                     /translation="MTLPPKSDKKQIKKIYAVGSGKGGVGKSSVTSLLAIAAARQGKR
                     VGILDADITGPSIPTFFGLHTFPFGNAEGIEPPASRSGIRVISMNLLLDDPAKPVIWR
                     GPLLGNAVTQFFNDVQWGELDVLFVDLPPGTADAVLTVMQTIPLDGFLLVTSPQELAS
                     LVVRKAAKMAEMLDIPLVGVVENMSSLRCPHCGERIELFGGSHAEALINDVKATFWGS
                     LPLDTEMAALGDEGHLEEYTNTEVFSVLEKGLANLL"
     gene            complement(1957..2292)
                     /locus_tag="PK849_09730"
     CDS             complement(1957..2292)
                     /locus_tag="PK849_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPE66443.1"
                     /translation="MAKIGIPSLGTDEASAVADRFARAPYFMIADEKGAILESFENTA
                     GSEAHGAASKALMALSQKEVSVLLVPRLGPNAIGFAKQAGMSAFCAEGLTVKDALAKY
                     AQGSLEKLL"
     gene            complement(2294..3166)
                     /locus_tag="PK849_09735"
     CDS             complement(2294..3166)
                     /locus_tag="PK849_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66444.1"
                     /translation="MSPREIVVVSGKGGTGKTTLTACFAALAEGPRIMADADVDAPNL
                     ELLLSPRETEKRPFFGMEVAHVDAARCTGCGECERFCRFRAISVRDGKAEVDSGFCEG
                     CGGCLLVCPEACISKEKFQRGVLRRGETDFGSMWYARLTPGGENSGMLVALLRKEAKR
                     EAVERGVSLLLVDGPPGVGCPVISSLTGADFAVLVTEPSRSALSDLRRVISLCTMLSV
                     PFGIAVNRWDLSKEMTSAIRAACDEEGWSFLGAVPFSEKTARAVNEGRIPVKEIGDPL
                     RELWRAILEKGREH"
     gene            complement(3163..>3549)
                     /locus_tag="PK849_09740"
     CDS             complement(3163..>3549)
                     /locus_tag="PK849_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HPE66445.1"
                     /translation="AGESLHADDWIMDAPPGASCPVSAVLRAADAAILVVEPTPFGLS
                     DVAGVLEVVADMKKPAGIVANKTGMGDLSLDDLCRRFSIEVLARYPFTRSAAEAGARG
                     VCPYRSEETWVWKTESLWRAIERRFS"
BASE COUNT          724 a         1196 c          997 g          632 t
ORIGIN      
        1 gacgtaccag tgattcatcc ccagcgcctg cggaatggtg atcttcccgt aattccccat
       61 ggtgaggagc gtccggctca tccacggaaa ggcctcggcg taaagcgccg cgccgacgag
      121 gccgcccgcg atcccccaga gcgcgtcgag acgcccctcc ccgaccgccg ccacggaagt
      181 gccggggcag taacccagca gcccccagcc gacgccgaag agaaggccgc cgaccacgac
      241 gccgcccgcg atcatcggct ttacggaaag tttcaccatt cccatgtcaa ggaggatgta
      301 gacgccgacc atcgcgacca cgatggacga cagcatgaat ttcagtatgg tgaagtcctg
      361 cacgcgaagg gcgccgagct gcttgtcgta ccggatcacg cgccccctct gcagcaggaa
      421 gccgaagaga agccccgtga cgaggccgta aaccatatta ggatccagtc ccgccattgc
      481 gctcgcctcc tccgtacaca agtttcgccg taaccatgcc ggcgatgtag aacgccgcaa
      541 acgcgacgat gccgctgacc gcaagctgaa gcgaaccgct cagcccgtgt ccgctgggtc
      601 acccgtccgc gagacgcgcg ccgaacattg cgacgacgcc gccgatgaag gcaacaaccc
      661 cgcgcttcaa cacgccgtca ccgaaccgct tcttccacat cggcggaacg agctcgaact
      721 tgaagtcgcc aaaaagtgcg gccgcaacca gcgaaccgat gaaaatgccc acaaggaacc
      781 acgcctgcca gtccatcttc ggggccgtct tcaccaggta ttcaagcgtc gccgcccgct
      841 ccggaagcac cgcctgctcc accgccgcag tcgcccggac aaaggttgtg gacgccccca
      901 gatacttccc ggcgaaccag accgagccta tggaaacaag tccggacaat cctccggcga
      961 ggtaggggtt ccatccgccc tcttctttca tcgtatcccc tccattctcc agataatatt
     1021 acaaatactg cacttcctta caatttaatt atatctgctt atattgcatt gtcaaccttt
     1081 cacattaaaa aaagagcccc ggagggctct tcactcttct tgccggagag agcgctacaa
     1141 caggttggca agccccttct ccaacacgga gaagacttcg gtgttggtgt actcctcaag
     1201 gtgcccctcg tctcccagcg cggccatttc cgtatccaga ggaaggctcc cccagaaggt
     1261 ggccttcacg tcgttgatca gcgcctcggc gtggcttccg ccgaaaagct cgatccgctc
     1321 gccgcagtgc gggcagcgaa ggctgctcat gttctccacc acgccgacga ggggaatatc
     1381 cagcatctcc gccatcttcg cggccttgcg gaccacgagg gaggcaagct cctgcggcga
     1441 ggtcaccagc aggaagccgt cgagcgggat agtctgcatc acagtgagca cggcgtcggc
     1501 ggtgccggga ggaaggtcga cgaagagcac gtcgagttcg ccccactgga cgtcgttgaa
     1561 gaactgggtc acggcgttcc cgaggagagg gccccgccag atcacaggct tcgccggatc
     1621 gtcgagcaac aggttcatcg agatgacgcg gattccggaa cgcgatgccg gaggctcgat
     1681 cccctcggcg ttgccgaacg ggaaggtgtg caggccgaag aacgtcggga tggacggccc
     1741 cgtgatgtcg gcgtccaata tgccgaccct cttcccctga cgggcggctg cgatcgccag
     1801 gagcgacgtc acggagctct ttcccactcc gcccttgccg cttccgacgg cgtagatttt
     1861 cttgatctgt ttcttgtcgc ttttcggtgg cagtgtcata aaaaaggttc gcccctttgt
     1921 cgtattatgg atgctacagt ctattgtact cctgatttac agtaatttct cgagggaacc
     1981 ctgcgcatac ttcgcgagcg cgtctttcac agtcagtcct tcggcgcaga acgcgctcat
     2041 cccggcctgt ttggcaaagc cgatcgcgtt cggtcccaga cgcggaacca gcagcacgga
     2101 gacctccttc tgcgaaagcg ccatcagcgc tttggaagcg gccccatggg cctcgcttcc
     2161 ggccgtgttc tcgaaggatt ccaaaatcgc gcccttttca tcggcgatca taaaatacgg
     2221 ggcgcgggcg aaacggtccg ccactgcgct tgcctcatca gttcccaaag aggggattcc
     2281 tatttttgcc atatcaatgt tcacgtcctt tctcgagtat agcccgccac agttcgcgca
     2341 gcgggtcgcc aatctccttc accgggatgc gtccctcgtt gacggctctc gccgtcttct
     2401 ccgaaaaagg cacggcgccg aggaaggacc acccctcttc gtcacaggcg gcgcggattg
     2461 cgctggtcat ctctttcgaa agatcccagc ggttcaccgc gatgccgaag ggaaccgaaa
     2521 gcatcgtaca gagggagatc actcttcgca aatccgaaag cgccgagcgc gaaggctcgg
     2581 tcaccagcac cgcaaagtcc gctcccgtca acgaagagat aacgggacat cccacgccgg
     2641 gaggtccgtc cacgagaagg agcgaaacgc ctctttcaac ggcttcacgc ttcgcctcct
     2701 tgcgcagaag ggcgaccagc ataccggagt tctcgccacc gggcgtcaga cgagcatacc
     2761 acatcgaacc aaaatccgtc tcgccgcggc ggagcactcc tcgctggaac ttctccttgg
     2821 agatgcacgc ctcggggcag acaagcaggc atcctccgca cccttcgcag aaaccggagt
     2881 ccacctccgc ctttccgtcc cggacggaga tcgccctgaa gcggcagaag cgctcgcact
     2941 ccccgcatcc cgtgcagcga gcggcgtcca catgcgcgac ctccataccg aagaaggggc
     3001 gcttttccgt ctctcggggg gaaagaagca gctccaggtt cggcgcgtcc acgtcggcgt
     3061 cggccatgat cctcggcccc tccgcaagcg ctgcaaaaca ggccgtgagg gtcgtcttcc
     3121 ctgtgccgcc cttgccgctg acgacaacga tctcgcgcgg actcatgaga atctcctttc
     3181 gatagcacgc cagagggact ctgtcttcca tacccacgtt tcctcggagc ggtacgggca
     3241 gactcccctg gccccggcct ccgcggcgga gcgggtgaag gggtagcgcg cgagcacctc
     3301 gatggagaag cggcggcaca ggtcatccag cgatagatcg cccatgccgg tcttgttcgc
     3361 gacaattccc gctggttttt tcatgtctgc gacgacttcg agcacacccg cgacatccga
     3421 aagaccgaac ggcgtcggct cgacgacaag gatggcggcg tccgctgcgc gcagcaccgc
     3481 cgacacggga caagaagcgc ccggaggcgc atccatgatc cagtcgtcgg cgtgcagcga
     3541 ctcgcccgc
//
LOCUS       DAOPKU010000307         3545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29375_length_3545_cov_4.426648, whole genome shotgun sequence.
ACCESSION   DAOPKU010000307 DAOPKU010000000
VERSION     DAOPKU010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29375_length_3545_cov_4.426648"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..583)
                     /locus_tag="PK849_09745"
     CDS             complement(8..583)
                     /locus_tag="PK849_09745"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66446.1"
                     /translation="MKSKRIGFLFFFLPVALWLGLLIVLPHLELLRLSFTSTRESGFT
                     LANYFAFFNEPIYWLTFVRTALYSILVTFIVMVVALPVAFYITKVAHVRVSGFLMVLV
                     LVPFWVSELIRVYGWIILLRESGVLNNFLVSIGLFEKPIEMLYNDATMIMGLVYTSML
                     FMVVPVIGVMDALDDSLIEAAYDLGASKTAI"
     gene            complement(573..1688)
                     /locus_tag="PK849_09750"
     CDS             complement(573..1688)
                     /locus_tag="PK849_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66447.1"
                     /translation="MRNALSVQNLVKRFGTFTAVNNVSFDVEDGKFFSILGPSGCGKT
                     TLLRMIAGFYQQDEGTILFNGQDMEGVAPNKRPVNLVFQNLALFPMMNVHENVAFGLR
                     RRGEKGAAVERKVKDMLERVGLPESGKKEVSQLSGGQKQRIAIARCLVLEPTVLLLDE
                     PLGALDAKLREHMKVELKTLQAKVGTTFVYITHDQSEAMVMSDRVAVMNNGAFEQIGT
                     PQELYHAPSSSFVAQFVGANNKLSVMLRRGEAGELLARYANGLDFRLAPACSIDAQPD
                     EPHDLFVRPESMVINPPADDGRFNSLPVTVKAILFDGGNSRLLVSSEGTTDEFTVALP
                     QTRQFDDIKVRDTIRVGWDPSASLCFRKSDWKLYDEE"
     gene            complement(1772..2842)
                     /locus_tag="PK849_09755"
     CDS             complement(1772..2842)
                     /locus_tag="PK849_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPE66448.1"
                     /translation="MKRFLIVLCVFALMFSVLAPASSEASVLRVITWSGYAPQELLDK
                     FTAETGIKVEVTLSNNEEMISKLRATRGGGFDLAQPSQDRISSVVEQYKIYQPIDFSK
                     IDEAQIDPSLLEAVKKNTLVDGKSYAVPHVYGTEGLVVNKAKAPNIKDFKDLLDPQYA
                     GRVSYRLKRPTLIGMGFSLGYDPFSLYTKTEEYQKFLDEMEKVLIAGKPAVKTYWDNG
                     DQLLQLLRSGEVWAASAWEQAGWKLHAENPDIDYIAPTSGALAWVDTFAIPARSENLE
                     AAYAYINFMLRPENAAFFTNRETYGTASKDAVKHLDPEIKANFERGLPPEVLAKINWY
                     PTVPAGVEEMEGKTLDKVKAAR"
     gene            3135..>3545
                     /locus_tag="PK849_09760"
     CDS             3135..>3545
                     /locus_tag="PK849_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPE66449.1"
                     /translation="MEKKTILVLTGSPRKGGNSDMMAEAFIEGATSVGHEVLRFDAGR
                     NTVNGCRACGACWAKGSACVFEDGFTELAQLLVKADVLVFSTPLYWFNFPSQLKAAID
                     KMNAFLKPNCPTPLKIRESVLMVCAHDTDDDLFDG"
BASE COUNT          660 a         1063 c         1157 g          665 t
ORIGIN      
        1 gatcgtgcta gatggccgtt ttcgacgcgc cgaggtcgta cgccgcctcg atcagcgagt
       61 cgtcgagcgc gtccatcacg ccgatgaccg gcaccaccat gaagagcatg ctggtgtaca
      121 ccagccccat tatcatcgtc gcgtcgttat agagcatctc gatcggcttc tcgaacaggc
      181 ctatcgaaac gagaaagttg ttcagcacgc cgctctcgcg aagcaggatg atccagccgt
      241 agacgcggat cagctcgctc acccagaagg ggacgaggac gagcaccatc aggaagccgc
      301 tcacgcgcac atgtgcgacc ttcgtgatgt agaaggccac ggggagcgcg actaccatca
      361 cgatgaaggt gaccaggatg gagtagagcg cggtgcggac gaaggtcagc cagtagatcg
      421 gctcgttgaa gaaggcgaag tagttggcga gcgtgaagcc gctctcgcgg gtggaggtga
      481 aggagaggcg cagcagctcg aggtgcggga ggacgatcag cagcccgagc cagagggcca
      541 ccggcagaaa gaaaaagaga aagccgatcc gtttactctt catcgtacag cttccagtcg
      601 ctcttccgga aacagagcga ggccgacgga tcccacccca cgcggatggt atcgcgtact
      661 ttgatgtcgt cgaactgtcg cgtctgcggc agcgccacgg tgaactcgtc ggtcgtcccc
      721 tcggacgaga ccagcaggcg gctgtttccc ccgtcgaaga ggatcgcctt caccgtcacg
      781 ggcagcgagt tgaagcggcc gtcgtcggcg ggcgggttga tcaccatcga ctccgggcgg
      841 acgaagaggt cgtgcggttc gtcgggctgc gcgtcgatgg aacaggccgg agcgaggcgg
      901 aagtcaagcc cgttcgcgta ccgtgcgagc agctcgcccg cttcgcccct gcggagcatg
      961 acggagagct tgttgttggc gccgacgaac tgcgccacga acgaggaaga tggcgcgtgg
     1021 tacagctcct gcggcgtgcc gatctgctcg aaagcgccgt tgttcatcac ggcgaccctg
     1081 tcgctcataa ccatcgcttc ggactgatcg tgcgtgatgt agacgaacgt cgttcccacc
     1141 ttggcctgga gcgtcttcag ctccaccttc atgtgctcgc ggagcttcgc gtcgagcgcg
     1201 ccgagcggtt cgtcgagcag cagcaccgtc ggctccagca ccaggcagcg cgcgatggcg
     1261 atgcgctgct tctgcccgcc cgagagctga ctgacttcct tcttcccgga ctccggcagg
     1321 ccgacccgtt cgagcatgtc cttcaccttc cgctccacgg cggccccctt ctcgccgcgg
     1381 cgccgaaggc cgaacgcgac gttttcgtgg acgttcatca tcgggaagag ggcgaggttc
     1441 tggaagacga ggttcacggg gcgcttgttc ggggccacgc cctccatgtc ctgcccgttg
     1501 aagaggatgg ttccctcgtc ctgctggtag aagccggcga tcatccggag cagggtggtc
     1561 ttgccgcacc ccgaggggcc gaggatcgag aagaacttgc cgtcctccac gtcgaaggag
     1621 acgttgttca ccgcggtaaa agtgccgaat cgtttcacga ggttctgtac tgaaagagcg
     1681 ttgcgcatac ggcggtcacc tctgaatgtg cagcaggcgg cggcctcttc gcgaagaccg
     1741 ccgcctgccg gcaggataga tgacggctgc gttatctggc cgcctttact ttatcgagcg
     1801 tctttccttc catctcctcc acgcccgccg gaacggtggg gtaccagttg atcttcgcga
     1861 gcacctcggg cggcaagccg cgctcgaagt tcgccttgat ctccgggtcg agatgcttca
     1921 cggcgtcctt ggaggctgtg ccgtaggtct cgcggttggt gaagaaggcc gcgttttcgg
     1981 ggcggagcat gaagttgatg taggcgtagg ccgcttccag gttctcggag cgcgccggaa
     2041 tggcgaaggt gtcgacccag gcgagcgcgc cgctcgtggg ggcgatgtag tcgatgtcgg
     2101 ggttctcggc gtggagcttc catccggcct gctcccacgc ggacgcagcc cagacttcgc
     2161 cggagcggag cagctggagc agctggtcgc cgttatccca gtacgtcttc acggcgggct
     2221 tgccggcgat gaggaccttc tccatctcgt cgaggaactt ctgatactct tcggtcttgg
     2281 tatacaggga gaacgggtcg taccccagcg agaagcccat gccgatcagg gtggggcgct
     2341 tcaggcggta ggagacgcgc ccggcgtact gcggatcgag caggtccttg aagtccttga
     2401 tgttcggggc cttcgccttg ttgaccacga gcccttccgt gccgtagacg tgcggcactg
     2461 cgtaggactt gccgtcgacc agcgtgttct tcttcaccgc ttccagcagc gacgggtcga
     2521 tctgcgcctc gtcgatcttc gagaagtcga tgggctggta gatcttgtac tgctccacga
     2581 cggaggagat gcggtcctgc gagggctggg ccaagtcgaa gccgccgccg cgggtcgcgc
     2641 gcagcttgct gatcatctct tcgttgttgc tcagcgtcac ctcgaccttg attcccgtct
     2701 cggcggtgaa cttgtcgagc agctcctgcg gggcgtaccc cgaccacgtg atcacccgaa
     2761 gcacggacgc ctcggacgac gcgggggcaa gtacggagaa catgagcgcg aaaacgcaca
     2821 gcacgatcaa aaacctcttc attcatatcc ctcctgttgt agtgtggtga actttggaac
     2881 ttccgaaagg cctgcgggtt ccctcccctg cgcgggggtc tgtagcgaac ccgtacatac
     2941 gattctagta gttcaggggg gcgaagtcaa cagaaatttg tttcaaatcg gttacaacac
     3001 gacgccggaa agagtggacg gcgatcccga aatgctcgcg gcaaagagta taatagcggt
     3061 cggggttcgc cccggaacga ccggaaagcg tcgccgggaa gagcgttcga tacagccgaa
     3121 ggggagtgca tactgtggaa aagaagacga ttctcgtcct gaccggaagt ccgcgcaagg
     3181 gcggcaacag cgatatgatg gcggaggcct tcatcgaggg cgcaacgagc gtcgggcatg
     3241 aggtgctcag gttcgacgcg gggcggaaca cggtgaacgg ctgccgcgcg tgcggcgcct
     3301 gctgggcgaa ggggagcgcc tgcgtcttcg aggacggctt cacggagctt gcgcagctgc
     3361 tggtaaaggc ggatgtgctc gtcttctcca cgccgctcta ctggttcaac tttccctcgc
     3421 agctcaaggc cgccatcgac aagatgaacg ccttcctgaa gcccaactgc ccgacgccgc
     3481 tcaagatcag ggagagcgtc ctcatggtct gcgcccacga cacagacgac gacctcttcg
     3541 acggc
//
LOCUS       DAOPKU010000308         3544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29389_length_3544_cov_4.940097, whole genome shotgun sequence.
ACCESSION   DAOPKU010000308 DAOPKU010000000
VERSION     DAOPKU010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29389_length_3544_cov_4.940097"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PK849_09765"
     CDS             <1..445
                     /locus_tag="PK849_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HPE66450.1"
                     /translation="VAAIELKSHVGPSFGNNFNNRTEEATGSACDFWTAFREGAFGAQ
                     PRPFVGWLMLVEDAPGSSSPVKDKSPHFPVFEEFQGASYLKRYELLCRRLMQEGLYTT
                     AAVLSTPRNALETEAYSELSPMTGLRQFVAALAGHVAAEAARIGG"
     gene            complement(831..2600)
                     /locus_tag="PK849_09770"
     CDS             complement(831..2600)
                     /locus_tag="PK849_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03768 family metallophosphoesterase"
                     /protein_id="HPE66451.1"
                     /translation="MNSLYPYKIMGFIFFTCMLFFFFPGRSECAVNSQSEPEGYPIAS
                     DVQTTVQRTIVPGATPTTAISLREISKYEQYGYGDWTYGPGLPHDRRLDLMPKEYNSN
                     GTTQKTRLLNFFTISDIHITDKESPNQLIHLQNLHPESRYGSSLYSGVMLYTTHVLDA
                     AIQTVNALHKQNPLDFGISLGDACNTSQYNELRWYIDVLDGKVIRPSSGAHLGAETID
                     YQKPYKAAGLDTSIPWYQTMGNHDHFWMGSFPLEHRLRKDLRQSYVSDDVFATGDLLS
                     DPRKIGSRDYYMGVLDGSTPYGDVKYAGPVGDFKTQPKVAADPDRRSILRTEWMQEFF
                     NTSSLPAGHGFDMPGAKDGFACYSFVPKPNVPLKMIVLDNTQRDDSGSLDIHGHGFLD
                     AARWEWLKKELADGTAAGQLMVIASHIPISVEVTAPNSSMGWWTDPKNAVTLPDLLAE
                     LHSHPNLVLLMAGHRHLNTVKAFVSPDPAGAPENGFWQVETSSLHDFPQQFRTFEIFL
                     NSDDTISIVTTNVDPAVLEGTPAAASRRYAIAAAQILKNMEGIEHWNPTNDPTVKPMP
                     AGSYNAELVVPLSPAMREKLKNF"
     gene            3095..>3544
                     /locus_tag="PK849_09775"
     CDS             3095..>3544
                     /locus_tag="PK849_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPE66452.1"
                     /translation="MASRLTKTRTLAAVAVAALVGAGYFIWGAVPAKREQTEVARVAR
                     PVKTMAVRAAGGGGERIFPGKVLASQKVNLAFRVSGQIAELPAVKGTFVEPGTLLARL
                     DPRDFEVQLANAKSELGNAKARLEAMKAGARKEEIAMLTSKVNSARAQ"
BASE COUNT          754 a         1000 c         1040 g          750 t
ORIGIN      
        1 cgttgcggcg atcgagctga aaagccacgt cggcccttcc ttcggcaaca atttcaacaa
       61 tcggaccgaa gaagccaccg gcagcgcgtg cgacttctgg acggcgtttc gggaaggagc
      121 tttcggcgca caacctcggc cgttcgtcgg ctggctgatg ctggtcgaag acgctcccgg
      181 atcaagttca cccgtaaagg acaaatctcc tcattttcct gtgttcgaag aattccaggg
      241 agcttcatac ctgaaacggt acgagttgct gtgccggcgt ctgatgcagg aaggactcta
      301 tacaaccgcc gcagtgctct cgacaccgcg aaacgccttg gaaacagagg cctactccga
      361 actttctccc atgacggggc tgagacaatt tgtcgcggca ctggccggac acgtcgcagc
      421 ggaagccgca cgaatagggg gttgatgact ggatgtgaag cgtcctcgtc caagcgctca
      481 gtcggaaact ggtatgcgtc catttctaaa ctcctctagc caacgggaaa ggcatactct
      541 ctttcaaaaa gaggttgtaa caactgacga caacccttgc aaaggagggt tgtcgtcatg
      601 gagaacagta cccgcggcac ccgaacatcc ggaagcggca atatcggaga ttgtgcgcgc
      661 acacaggatc tttttcgccg ccgagggaac ttggaacgcg cggctcgccg cttcttcccg
      721 aagcgcctca agcataacta cggctttgac ctccgctgaa aagttccgac gcgctctgat
      781 tatttaaggg cgcaccctcc cccccggttt tcggggtcca ttcttctccg tcaaaagttt
      841 ttcagcttct cgcgcatcgc cgggctcagg ggaaccacca gctccgcgtt gtacgacccg
      901 gccggcatgg gcttcactgt gggatcgttt gttggattcc aatgctcgat tccttccatg
      961 ttcttgagaa tctgcgctgc cgcaatggca tatctgcgcg aggctgcggc gggcgtccct
     1021 tcaagaactg cgggatcgac gttcgttgtc acgatggaaa tggtatcgtc gctgttgagg
     1081 aaaatctcga aagtgcgaaa ctgctgcgga aaatcgtgaa gcgacgacgt ttccacctgc
     1141 cagaagccgt tttcgggcgc gcccgcagga tccggcgaca cgaaggcttt cacggtattc
     1201 agatgacgat gcccggccat caacaagacg aggttcggat ggctgtgcag ttccgccagc
     1261 agatcaggca gtgtgacggc atttttcgga tcggtccacc agcccatcga tgaattgggc
     1321 gctgtcactt caacgctgat cgggatgtgc gaggcgataa ccatcagctg accggccgct
     1381 gtgccatcgg caagctcctt tttcaaccat tcccagcgcg ccgcatccag aaagccgtgc
     1441 ccgtgaatat cgagggagcc gctgtcgtcc ctttgcgtat tgtccagcac gatcatcttg
     1501 agcggaacat tcggtttcgg aacgaagctg tagcatgcaa agccgtcctt ggcgccgggc
     1561 atgtcgaacc cgtgaccggc gggaagcgac gacgtgttga aaaactcctg catccactcc
     1621 gttcgcaaaa tcgaacggcg atccggatcg gcggcgactt tgggctgggt cttgaaatcg
     1681 ccgacagggc ccgcgtattt gacatctccg tagggcgtcg agccgtcgag cacccccata
     1741 tagtagtcgc gactgccgat cttccggggg tcgctcagga gatctcccgt ggcgaagacg
     1801 tcatcgctga cgtaggattg ccgaagatcc ttgcgcagcc tgtgctccag cgggaaggag
     1861 cccatccaga aatgatcgtg gttccccatt gtctgatacc agggaatgga cgtgtcgagc
     1921 cccgccgctt tgtagggttt ctgataatcg atggtttcgg ctcccagatg cgcgccggag
     1981 ctgggccgga taaccttgcc gtcgagaacg tcgatatacc accgaagttc attgtactgc
     2041 gaggtgttgc aggcgtctcc aagagaaata ccaaagtcca gagggttctg cttgtgcagc
     2101 gcgttgacgg tctggatggc ggcgtcgagc acgtgcgttg tgtacagcat gaccccggaa
     2161 tacagagaag atccgtaccg tgattcagga tgcaggtttt ggagatggat cagctgattg
     2221 ggagattctt tgtcggtgat atggatatcc gatatggtaa agaaattcag cagccgtgtt
     2281 ttttgagtcg tgccgttgct gttgtactcc ttcggcatga gatcgagccg tctgtcgtgg
     2341 ggaagccccg ggccgtaggt ccaatcgccg tagccgtact gttcgtattt ggaaatctcc
     2401 cggagactga tcgcagtcgt cggggtcgcc cccggcacga tcgtcctctg aaccgttgtc
     2461 tgcacgtcgg aggcgatcgg gtatccttcg ggctcgcttt gactgttcac ggcgcattcc
     2521 gaacgcccgg gaaaaaagaa aaacagcata catgtgaaga agatgaaacc cattattttg
     2581 tacggataca atgaattcat accaacactc tccctgatta ccaaagaatt tgtgaatctg
     2641 ttgcgaaggc atcggcaggt tcatcggccg cccgtctccg ccagtctctg cccgatgcgt
     2701 tgaaacaccg aacccaaagc ccgaatactc tgcgccgctc cccatcccgc atctcctccg
     2761 actgcattgt acagtcgatt acgcgcatcg gcaaactcga atttcagtct gcacaccata
     2821 cgccgacgtc cggggaggga tatacgtcgc cacaatcgtc ggcgagaact ggatgatgga
     2881 gaaaatgaag tcgggctgga tcagaacact ggcggatctt ctcaagtaga cgccgacgcg
     2941 aaaaaataca ccgcgctgcg gagtgaaagg ttgaagacgc tctttccggg gaccggaccg
     3001 gggtccccgt tttttgtgtg tcggttataa tagcgaaaag agggcttctc tttttcttat
     3061 aaactatttc ttgatgcgaa agggtggttt aaaaatggcg agcagactca caaaaacgcg
     3121 cactctcgcg gcggtcgcgg tcgctgcgct ggtcggcgcg gggtatttca tttggggcgc
     3181 cgtccccgcg aagcgcgagc aaaccgaggt cgcccgcgtc gcccgtccgg tgaagacgat
     3241 ggcggttcgc gccgcaggcg gcggaggaga gcgtatcttc cccggcaagg tgctcgcgtc
     3301 gcagaaggtg aacctggcct tccgcgtctc cggtcaaatc gcggagctgc ccgcggtgaa
     3361 agggacgttt gttgagccgg gcacgctgct ggcccgtctc gacccacgcg acttcgaggt
     3421 gcagctcgcc aacgcgaaga gcgagctggg gaacgcaaag gcccgcctgg aagcaatgaa
     3481 ggccggagcg cgcaaggagg agatcgccat gctcacctcg aaggtgaact ccgcccgcgc
     3541 ccag
//
LOCUS       DAOPKU010000309         3540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29434_length_3540_cov_3.937159, whole genome shotgun sequence.
ACCESSION   DAOPKU010000309 DAOPKU010000000
VERSION     DAOPKU010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29434_length_3540_cov_3.937159"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3237)
                     /locus_tag="PK849_09780"
     CDS             complement(<1..3237)
                     /locus_tag="PK849_09780"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPE66453.1"
                     /translation="MSNFAFLPEDFRDIAESAVRAEGYVSGDPRAACFHARFALEAAV
                     HWLFRRDPALRMPYDDSLGALLHEPTFQNLLPQAVFQKARVIQRMGNRAVHDTRPVRA
                     SDALLAVKELYHVCYWLTRTYVPGAVPPDAAWRDDRVPEARGGDAVPRAELEALEEKL
                     AGQSRLALEERRERDALDAEVRELRARLAEIRAASEAVPDMHDYSEAETRRWLIDAEL
                     RRAGWPLDSPRDREYRVTGMPNAQGEGFADYVLWGDDGKPLAVVEAKRTTADPAAGRQ
                     QAKLYADCLEAMHGQRPVIFFTNGYETWLWDDAFYPPRTVAGFFTKDELARLILRRSQ
                     RGALDVAQVRDAIVERYYQKRAIGSIFAQFAASRRKALLVMATGTGKTRTAIALVDAL
                     QRAGWVKRALFLADRVSLVHQAVNAFKRHLPESSPVNLVTDKEAEGRVFVCTYPTMMG
                     LIDTTDGEAARFGPGHFDLVIIDEAHRSVYQKYGAIFRYFDSLLVGLTATPRDDVDKN
                     TYDLFELEPGVPTDAYELETAVADGFLVPYKVRKPELKFPREGIVYDSLTDEEKAQWE
                     SLDWGDEAEERGLPERVNASAVNSWLFNCDTVDKALGYLMEHGHTVKGADRLAKTILF
                     ARNHEHAQFVERRFDAHYPHYAGSFARVIDNYARYPQSLIDDFAQPDKPPHIAISVDM
                     LDTGIDIPEVANLVFFKPVYSRIKFWQMIGRGTRLCPDLFGPGRDKEDFRIFDFCGNF
                     EFFGENPEGMEATDAAPLGVRLFRSRVRLLEHVRKAPELDPGGTLDGALVRVLHGEVA
                     AMNPRNFIVRMHLQAVERFRKREAWNALSGDDYELLHREVAGLPGEAEEDAIESRLFD
                     LTALSMQLALVEEDRGGFERRRRRVTEIAAMLEEKAAIPAVKAQLAYLSALQEVSFWE
                     GITLDMLEEMRLRLRGLVAFLDRKTRTLVYTDFEDELLDEGDESAPVMPKMTGAQYEK
                     KVKDYLRTHLDHIVIHRLRANMPLTPIDLEELERTLAAIGEEDGGALLEGMLARSNAP
                     SLAWFVRTLVGMDRAAAQAAFSAFLNDRSLTPQQIRFVETIIDQLTAHG"
     gene            complement(3234..>3540)
                     /locus_tag="PK849_09785"
     CDS             complement(3234..>3540)
                     /locus_tag="PK849_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66454.1"
                     /translation="NPTHLNDEIFNPCEDLNLLDIAAKVRNTGKADKQLIKETILELC
                     RDRFLTPQQLGKLLNRSYMSLRERYIKQMVEEMLLERRFPNQPSHVNQAYRTREPSP"
BASE COUNT          609 a         1226 c         1055 g          650 t
ORIGIN      
        1 gccgtgggcc gtaagctggt cgatgatcgt ttcgacgaag cggatctgct gcggggtcag
       61 gctgcggtcg ttgaggaacg cggagaaggc ggcctgcgcc gcggcccggt ccatccccac
      121 cagagtacgg acgaaccacg cgagcgacgg cgcgttgctc cgcgcgagca tcccctcgag
      181 aagcgctcct ccgtcctcct cgcctatcgc cgcgagcgtc cgctccagct cctccaggtc
      241 gatcggcgtc agcggcatgt tggcgcgcaa acggtggatg acgatatgat ccaggtgggt
      301 gcgaagatag tccttcacct tcttctcgta ctgcgccccg gtcatcttcg gcatgacggg
      361 ggcggactcg tccccttcgt cgagcagctc gtcctcgaag tcggtgtata ccagcgtgcg
      421 cgtcttcctg tcgaggaagg caaccagccc tcgtagacgg agacgcatct cctccagcat
      481 gtcgagcgta atcccttccc agaacgaaac ctcctgaagc gctgaaagat aggccaactg
      541 agccttcacc gccggaatcg cggccttctc ctccagcatc gccgcgatct cggtcacgcg
      601 ccgcctgcgc cgctcgaacc cgccccgatc ttcttcgacg agcgcaagct gcattgagag
      661 ggccgtcaga tcgaacagcc gcgactcgat cgcgtcctcc tccgcttccc ccggaagccc
      721 cgcgacctcc cggtgcagca gttcatagtc gtccccggag agcgcgttcc acgcctcgcg
      781 cttccggaaa cgctccaccg cctgaaggtg catccgcacg atgaagttcc gcggattcat
      841 cgccgccacc tcgccgtgca gcacgcgcac aagcgcgccg tcgagcgtcc ctccggggtc
      901 cagttcgggg gccttcctga cgtgctccag caaccgtacg cgcgagcgga agagacgcac
      961 gccgagcgga gcggcgtcgg tcgcttccat cccctccggg ttctcgccga agaactcgaa
     1021 gttgccgcag aagtcgaaga tacggaagtc ctccttgtcg cgccccggcc cgaagagatc
     1081 cggacagagc cgcgtgccgc gcccgatcat ctgccagaac ttgatcctgg agtagaccgg
     1141 cttgaagaag acgagattcg ccacctcggg gatgtcgatg ccggtgtcca gcatgtccac
     1201 ggagatggcg atgtgcggcg gcttgtcggg ttgggcgaag tcgtcgatca ggctctgcgg
     1261 ataccttgcg tagttgtcga tcacgcgcgc gaagctcccc gcgtagtgcg ggtagtgcgc
     1321 gtcgaagcgc cgttcgacga actgcgcatg ctcatggttc cgggcgaaga gaatcgtctt
     1381 ggcgagccgg tcggcgccct tcaccgtatg cccgtgctcc atcagatacc cgagcgcctt
     1441 gtccaccgta tcgcagttga agagccaact gttcaccgcc gaggcgttca ccctttcggg
     1501 aagccctcgc tcctccgctt cgtcacccca gtccaggctc tcccactggg ccttctcttc
     1561 atccgtcagg gaatcgtaga cgatcccctc gcggggaaac ttcagctccg gcttccgcac
     1621 cttgtacggg acgaggaaac cgtcggcaac cgccgtctcc agctcgtagg cgtcggtcgg
     1681 cacgcccggc tccagctcga agaggtcgta ggtgttcttg tccacgtcgt cgcgcggcgt
     1741 cgccgtcagt cccacgagca gcgagtcgaa gtagcggaag atcgcgccgt acttctggta
     1801 gaccgaacgg tgggcctcgt cgatgatcac gaggtcgaag tgccccggcc cgaagcgcgc
     1861 cgcctccccg tccgtcgtgt cgatcagccc catcatcgtc ggataggtgc agacgaagac
     1921 gcgcccttcg gcctccttgt cggtcaccag gttcacgggg ctcgactccg gcaagtgcct
     1981 cttgaaggcg ttcaccgcct gatgcaccag agaaacccgg tccgccagaa agagcgcgcg
     2041 cttcacccat ccggcccgct gcagcgcgtc caccagagcg atggccgtgc gcgtcttgcc
     2101 cgtccccgtc gccatcacga gcagcgcctt gcgccgcgat gccgcgaact gcgcgaagat
     2161 gctgccgatg gcgcgcttct gatagtagcg ttcgacgatg gcgtcccgca cctgcgccac
     2221 gtcgagcgcc ccccgctgcg aacggcgcag gatcagccgc gccagctcgt ccttcgtgaa
     2281 gaaccccgcc accgtgcgcg gcggatagaa tgcgtcgtcc cacagccatg tctcgtaccc
     2341 gttggtgaag aagatcaccg gccgctgccc gtgcatcgcc tcaaggcagt cggcgtacag
     2401 cttcgcctgc tggcgtcccg cggcggggtc cgcggtcgtc cgcttcgcct ccaccacggc
     2461 cagaggtttc ccgtcgtcgc cccagagaac gtagtcagcg aacccctcgc cctgtgcgtt
     2521 cggcatcccc gtaacccggt attcccggtc gcggggactg tcgagcggcc acccggctcg
     2581 gcgcagctcc gcgtcgatca gccagcgccg cgtttcggcc tccgagtagt cgtgcatatc
     2641 ggggacggct tcgctcgcgg cgcggatttc cgccagccgt gcacgcagct cgcgcacctc
     2701 cgcgtccagc gcgtcgcgct ccctccgctc ttcgagggca agcctgctct gcccggcaag
     2761 cttttcctca agcgcttcaa gttcagcgcg cggaaccgcg tccccgcccc ttgcctctgg
     2821 cactcgatca tcccgccatg cggcgtccgg cggcaccgcg ccgggaacgt aagtccgcgt
     2881 cagccagtag caaacgtggt acagctcctt caccgcgagc agtgcgtccg aagcgcgcac
     2941 aggacgcgtg tcgtgcacgg cgcggtttcc catccgctgg atcacccgcg ccttctgaaa
     3001 gaccgcctgc ggaagcagat tctgaaaagt gggctcgtgg agcagcgcgc cgaggctgtc
     3061 gtcgtacggc atccgaagcg ccgggtcgcg cctgaaaagc cagtgcacag cagcctccag
     3121 cgcgaagcgc gcatggaaac atgccgcgcg cggatcgccg gaaacatacc cctccgcccg
     3181 aacagcggac tcggcgatat cccgaaaatc ctccggaagg aaggcgaagt tactcatggc
     3241 gaaggctccc tggtgcggta ggcttgatta acatggctag gctgattggg gaaacgccgc
     3301 tctagaagca tttcttctac catctgcttg atatagcgct cacgtaggct catataagag
     3361 cgattcaaca acttaccaag ctgctgaggg gttagaaatc tgtctctgca cagttccagt
     3421 atcgtctctt ttatcagttg cttatcggcc ttacccgtat ttcgtacttt tgccgcgata
     3481 tccaaaagat taagatcctc gcacggattg aaaatttcgt cattcaaatg tgtggggttc
//
LOCUS       DAOPKU010000310         3531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29527_length_3531_cov_3.891254, whole genome shotgun sequence.
ACCESSION   DAOPKU010000310 DAOPKU010000000
VERSION     DAOPKU010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29527_length_3531_cov_3.891254"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PK849_09790"
     CDS             complement(<1..846)
                     /locus_tag="PK849_09790"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPE66455.1"
                     /translation="MHRFALKIYGCQMNVYDGDKIRTALVARGWRESPEDEADLVILN
                     GCSIRDKAEQKVWSDLGRFAVRWEQERKPTVAVTGCVAQSVGRRMAARFPWVRFVSGP
                     RHIGAVPDALEQFFNERDAGRTIGNEETVFLLDDDPRAFLDLPGAPTERVNRWKAFVT
                     IAHGCDNFCSYCIVPYVRGRFVSRPAEDILEEMRTLVADGVLEITLLGQNVNSYGSDF
                     SDGYRFSSLLRDAAASTGVRLLRFVTSHPRDFTPDIVEAMAEHTDILCPSINLPIQSG
                     SDRILK"
     gene            1067..1531
                     /locus_tag="PK849_09795"
     CDS             1067..1531
                     /locus_tag="PK849_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66456.1"
                     /translation="MRRNSCCSVLILFVLLCGGPALAAVSLPDIPGYTAEAMRSTPLS
                     APSGDFGVWLSRTYRSEGRRLLVATLMSGPGAGPLVTGEEGTKTDDRPLGFGSTYEVF
                     SLGGKPAVYETIPSVGVSLAVAVDANSTLTLESSSLGRDALEEAALCIMRAM"
     gene            complement(1607..1768)
                     /locus_tag="PK849_09800"
     CDS             complement(1607..1768)
                     /locus_tag="PK849_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPE66457.1"
                     /translation="MKKYVCTVCGYVYDPAEGDPDSGIAPGTAFEDIPEDWVCPVCAV
                     GKDMFEPEE"
     gene            complement(1897..3003)
                     /locus_tag="PK849_09805"
     CDS             complement(1897..3003)
                     /locus_tag="PK849_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPE66458.1"
                     /translation="MSFDHISRRLQRLRSVVAEEGMDVFVLLVTEGYNSESAAYISGF
                     RGSSCAVVISADRALLVTDGRYALQAKAQSPFSVVLQEQNTLQEKTAELLRGGGWTVA
                     GFEGERLSVSLFRAFEKSVPHWKDCSVLIPSLRRRKDELEIAAIAKAADIASASYEEV
                     LGLVREGMSETELNALLEYAVRKRGAEAGWKGSGFIVASGERSALPHGKATDRTFRKG
                     DIVTVDFGATVDGYMSDLTRNFSLGPLSEGAREIESVLLEAADKAAEALKPGAEGKFV
                     DSVARGIIASKGWGKHFSHGLGHGLGLEIHEAPRLSPVSKDILREGDIVTIEPGIYIE
                     GWGGMRIEDDYLVTSSGSVCISGGKGRKTAVVSV"
     gene            complement(3000..>3531)
                     /locus_tag="PK849_09810"
     CDS             complement(3000..>3531)
                     /locus_tag="PK849_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPE66459.1"
                     /translation="IRPGDVLILTKHAGLEGMAILANDRPDLLLPAIGPEGVEEARGW
                     LEQISVLEEAKLLRGMARFLHDPTEGGVGGGIAEIVRLSGLGVELDYGSVPFSPLTKK
                     AVQTLDFDPLHLISSGVLLAVVPQGGEADALAALREKGISGVAAGHFVEGAGNCPDDV
                     REELWGLLERPKERRE"
BASE COUNT          807 a         1075 c          916 g          733 t
ORIGIN      
        1 ttttaaaatc cggtcgctcc ccgactgaat agggagatta atcgaaggac agagtatatc
       61 cgtatgttcc gccatggctt cgacgatatc cggggtgaaa tcccgcggat gcgacgtgac
      121 gaagcggaga agacgcacgc ctgtggaggc tgctgcatct cgaagcaggg atgagaatct
      181 gtaaccgtcg gagaaatcgc tcccgtagct gttgacgttt tgtccaagga gggtgatttc
      241 aaggacgccg tcggctacaa gcgtccgcat ctcctccaga atatcctcag caggacggga
      301 gacaaatcgc ccccggacat acgggacaat acagtatgaa caaaaattgt cgcagccgtg
      361 agcgatggtg acgaatgctt tccagcggtt cacacgttcc gtaggagcgc ccggaagatc
      421 aagaaaagct cttggatcgt cgtccagaag aaaaaccgtc tcctcgtttc cgatggtcct
      481 tcccgcgtcc ctttcgttga aaaactgttc caaagcatcg ggaaccgcgc cgatgtgccg
      541 gggacccgac acgaaacgaa cccacgggaa acgggcggcc atcctgcgcc caacgctctg
      601 cgcaacacag ccggtcaccg cgacggtcgg tttgcgctct tgctcccaac ggacggcgaa
      661 acggccgagg tcgctccata ctttctgttc cgccttgtcc cgaatgctgc acccgttaag
      721 gatcacgaga tcagcttcgt cctcggggct ttctctccac ccgcgagcaa caagagcggt
      781 tcgtatcttg tcgccgtcgt agacgttcat ctgacatcca taaatcttca gtgcaaagcg
      841 gtgcaaagaa aaaactcctc ccgatgcgaa aatagaggta aaaacaacag aaatagtatt
      901 atataccctt caaagggaat tttggtacgt caaaaactca atccgtacgt tcacgcatcc
      961 tctttttcac caaggaagaa gaacggaaga aacgcgcaag gaaaaaccgt ttcgatagag
     1021 tacaatggta cccgggtttt gaacagagaa tcaaggaggt tgcgatatgc gaagaaacag
     1081 ttgctgtagt gtgctgatac tgtttgtact cctctgcgga gggccggccc tcgccgccgt
     1141 ttcacttccg gatatcccgg ggtatacggc ggaagcgatg cgcagtacgc ctctttcggc
     1201 tccatccgga gatttcggag tgtggctctc ccgaacctac cggtctgagg ggcggaggct
     1261 ccttgtagcg acgctgatgt ctggtcccgg agcgggtcct cttgtgaccg gagaagaggg
     1321 aacaaaaacg gacgaccgcc ctctgggctt cggctcgaca tatgaagtgt tcagccttgg
     1381 agggaaaccg gcagtatatg aaacaatccc ttcagtcggc gtctctcttg ccgttgctgt
     1441 ggacgcaaac agcacgctca cgctggagtc ttcctccctg ggcagggacg cgttggaaga
     1501 agctgcgctg tgtatcatgc gcgccatgta atcagctgct tttcaaaata aagaggggct
     1561 cctgtaaagg agcccttctt tccgcttcga gatcacatac gaaagactac tcttcaggct
     1621 cgaacatatc cttccccaca gcgcagacag ggcagaccca atcttcgggg atatcttcaa
     1681 aggccgtccc cggagcaatt ccggaatcgg gatcgccctc ggcgggatcg taaacgtaac
     1741 cgcatacggt gcacacgtac ttcttcatga tatttccccc ttcatcaatt cggaatcaga
     1801 tacaacttca ttatatcggg cgcagagaaa aaggcaactg cctgttgccg aaatccctgt
     1861 tccttctgtt gacaatcccc tgacgctcca gtggaatcag acggatacaa ccgcagtctt
     1921 cctgcctttc ccgccggaga tacagaccga accgctcgag gtcacgagat agtcgtcctc
     1981 gatgcgcata cctccccacc cttcgatgta gataccgggc tcgattgtca cgatatcccc
     2041 ttcacggaga atatccttcg aaacgggtga aagacgcggc gcttcatgga tctccaaccc
     2101 gagtccgtgc cccagtccgt gagagaagtg tttgccccac ccttttgaag cgattattcc
     2161 gcgggctacg gagtcgacga acttcccttc cgctccgggc ttcaaggcct ccgccgcttt
     2221 atccgccgcc tccagaagca cggattcaat ctcccgcgct ccctctgaca gcgggcccag
     2281 cgaaaagttc cgggtaaggt cggacatgta tccgtccacc gtagcgccga aatcgacggt
     2341 gactatgtct ccttttcgaa acgtcctgtc cgtcgccttt ccatgaggga gcgcgcttcg
     2401 ctctccggaa gcgacgataa agccgctccc cttccacccc gcctccgcgc ctctttttct
     2461 gacggcgtat tcgagaagcg cgttcagctc ggtctcggac attccctcgc gaacgaggcc
     2521 gaggacttct tcataggaag cagaggctat gtccgccgcc ttggcgattg cggcaatctc
     2581 caattcatct tttcgtcttc ggagcgaagg aatgagaaca gagcagtctt tccagtgcgg
     2641 gacggatttt tcaaacgccc gaaagagaga gacggagagc cgctcccctt caaaaccggc
     2701 gaccgtccac cctcccccgc gcagcagttc cgctgttttc tcctgaaggg tattctgttc
     2761 ctgcaaaacg actgaaaacg gggactgcgc cttcgcctga agagcatacc ttccgtcagt
     2821 aactaaaagc gccctgtccg cggatatcac gaccgcgcag ctgctccccc gaaaaccgga
     2881 gatgtaggcg gccgattcgc tgttatagcc ttcggtaacc aggagaacga agacatccat
     2941 accctcctcg gccacgacgc ttcgcagcct ctgaagacgg cgtgaaatat gatcgaaact
     3001 cattccctcc gctccttcgg cctctccaac aacccccaga gctcttcgcg gacatcgtcc
     3061 ggacagttgc ccgcgccttc gacaaaatgt ccggccgcga cgccggaaat acctttttca
     3121 cgaagagccg cgagcgcgtc ggcttccccc ccctgcggaa caaccgccag aaggactccg
     3181 gaagagataa gatgcagcgg atcgaaatcg agcgtctgta cagccttctt cgtaagagga
     3241 gagaacggca cgctcccgta atcgagttca actccaagac cgctcaagcg aacgatctcc
     3301 gctattccgc ctccgactcc tccctctgtc gggtcgtgga gaaaacgcgc catgccccgg
     3361 agaagcttcg cctcttcgag gaccgagatc tgttccagcc acccgcgcgc ctcctccacc
     3421 ccttcgggac ctatggcagg gagcagaagg tccggacggt cgttggccag aatagccatc
     3481 ccctccagcc ccgcgtgctt ggtgaggatg agaacatctc cgggacgtat a
//
LOCUS       DAOPKU010000311         3528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29557_length_3528_cov_4.671178, whole genome shotgun sequence.
ACCESSION   DAOPKU010000311 DAOPKU010000000
VERSION     DAOPKU010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29557_length_3528_cov_4.671178"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PK849_09815"
     CDS             complement(<1..711)
                     /locus_tag="PK849_09815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE66460.1"
                     /translation="MAHKPTYEELEAQVLRLKDAERELREMEEMLQDKITLWQLLIEQ
                     SKDGIVVLDENGSVYAANKRFAEMLGYTEEELRRLKIWEWDGRLSKEQLLGNLRRANT
                     SGHSLETLHRCKNGAIINVELSYNGALYNGRKLVFCVCRDISARKRMEQELRESEQKY
                     RELCIVDGLTNLYNARHFHSHLKMESNRADRYGQPLSLLFFDLDDFKKYNDVYGHVEG
                     DKVLSRIGEVVKRGLRQTD"
     gene            complement(838..1215)
                     /locus_tag="PK849_09820"
     CDS             complement(838..1215)
                     /locus_tag="PK849_09820"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPE66461.1"
                     /translation="MALLPEDVGRAWEKRKGAVVLTTVDKDGVPNSIYATCVSKYDEE
                     TIVVADNYFDKTQKNILAGSKGSVLFITDEGASFQIKGRIEYLKDGPVFDDMKKWNPT
                     KHPGHAAAALKVEEVYSGAKKLL"
     assembly_gap    1306..1405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1406..2343)
                     /locus_tag="PK849_09825"
     CDS             complement(<1406..2343)
                     /locus_tag="PK849_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPE66462.1"
                     /translation="MTRTNPSAASKHTEKRPRLGLALGAGAARGMAHLGVLQALEEAG
                     LAPDVVAGTSIGALVGGMYAAGKLDRLTEVALGLDWKQIARYFVEISFPRSGLIEGNK
                     LTALLAEIVGEVELTELALPFRAVTTNLMTGEEVVISDGDLVEAIRASIAVPGIFTPA
                     RRGDDLLVDGGLVNPVPVSVCRTMGAERIIAVDLNYGRVKDTRARNSTVKEKSALASQ
                     RPNFDAGQIFEWLERNTKQFDIELPNPLKAWMERHALPNVFDVLGNTLGIIESQIAAT
                     RLQVDKPDLLIQPNVGDIRFMDFVHAGEMIEEGRRAA"
     gene            complement(2340..>3528)
                     /locus_tag="PK849_09830"
     CDS             complement(2340..>3528)
                     /locus_tag="PK849_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPE66463.1"
                     /translation="QVNKIQGSREIPTPFECAQPRRVLFGRGRLREAGRIVREYGKRC
                     LVCTGSRPERAAELLDSLRGAGLVCELLTVSGEPSFESIRQATARAAEFSPDAVVAMG
                     GGSVLDTGKALAMLATNGGDPLDYAEIVGAGKPIMKTSLPLLAIPATAGTGSEATRNA
                     VLAHTEKQVKVSLRSRSMMPETVILDPHAMNGLPAHVIAASGMDALCQLIEAFVSCKA
                     NAWTDALCRAGIPMAFRALPKACDDPDDLGAREEMLLAACWSGVALANAGLGAVHGFA
                     GVVGGLTGAPHGLICATLLAPVCRANIAALGPEGAHPALGKYAELAEMLTGRDAEPEE
                     GTDELERLAARLPLRPFHVPEGTDGERVVAGAMAASSMKGNPVVLPKETLAALWRSVT
                     KTS"
BASE COUNT          530 a         1133 c         1044 g          721 t
ORIGIN      
        1 atcggtctga cggagaccgc gcttgacgac ctccccgatc cgcgagagca ccttgtctcc
       61 ctcgacatga ccgtagacgt cgttgtactt cttgaagtcg tcgaggtcga agaagaggag
      121 tgacaacggc tgtccgtagc ggtcggcgcg gttgctctcc atcttcagat gactgtggaa
      181 gtgccgtgcg ttgtagaggt tcgtgaggcc gtcgacgatg cagagttccc tgtatttctg
      241 ttcgctttcc ctgagctcct gttccatccg ttttcgtgcc gagatgtccc tgcacacgca
      301 gaacaccagc tttcgcccgt tgtagagcgc gccgttgtag ctgagttcga cgttgataat
      361 cgcgccgttc ttgcagcggt ggagcgtctc cagagaatgg ccggatgtat ttgcgcgccg
      421 gaggttcccc agcagctgct ccttcgagag gcggccgtcc cactcccaga ttttgagccg
      481 gcgcagctcc tcttccgtgt agccgagcat ttcggcgaag cgtttgttgg ccgcgtagac
      541 cgacccgttt tcgtcgagga cgacgatgcc gtccttcgac tgttcgatca aaagctgcca
      601 taacgtaatc ttgtcctgca gcatctcttc catttcacgg agttcgcgtt ccgcgtcctt
      661 caaccgcagt acctgcgctt ccagctcctc gtaggtcggt ttgtgcgcca tgacgctcac
      721 ctcgcttttt tgtcggttca ggaagagcat tatacaggcg ttcgcttgtt cgcgcagacg
      781 gggaaaatac tgaaaccggc cgggccgtcg cagagcagcg cggacgcggc cggttgatta
      841 cagcagcttc tttgcgcccg aatagacctc ttccaccttc aacgccgctg cggcatgtcc
      901 cggatgcttg gtcgggttcc attttttcat gtcgtcgaag accgggccgt ctttgagata
      961 ctctatgcgc cccttgatct gaaaggacgc gccctcatcg gtgatgaaga gcacggagcc
     1021 tttgcttccc gcgagaatgt tcttttgcgt cttgtcgaag tagttgtccg cgacgacgat
     1081 cgtctcctcg tcgtacttgc tcacgcatgt agcgtagatc gaattcggca cgccgtcctt
     1141 gtcgacggtc gtcaggacga ccgctccctt tctcttctcc cacgctctgc cgacgtcttc
     1201 cggtagtaag gccatgttgt tttcctccat taaagtaaag tagcggtatg gtgcagctca
     1261 gtaggaacaa taccacgaat accgcgccgt gcccagccgt tatttnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnngccgc acggcgtccc tcctcgatca tctcgcccgc
     1441 gtggacgaag tccatgaagc ggatgtcgcc gacgttgggc tggatcagca ggtctggttt
     1501 gtcgacctgc aatctggtcg ccgctatctg cgattcgatg atgccgagcg tgtttcccag
     1561 cacgtcgaag acattcggca gagcgtgccg ttccatccat gccttcagcg ggttgggaag
     1621 ctcgatgtcg aactgcttcg tatttcgctc cagccactcg aagatctgtc cggcgtcgaa
     1681 gttcggacgt tgcgaagcga gcgcgctctt ttctttgacc gtcgaatttc tggcgcgggt
     1741 atccttgacg cggccgtagt tcagatccac cgcgatgatg cgctcggcgc ccattgtacg
     1801 gcagacgctc accggcaccg gattcaccag cccgccgtcg accagcagat cgtcgcccct
     1861 gcgcgccgga gtaaagatgc ccggaaccgc gatgctcgcg cgtatcgcct ccacaaggtc
     1921 gccgtcgctg atgacgactt cttcgcccgt catgaggttc gtggtcacgg cccggaaggg
     1981 gagcgcaagc tccgtcaatt cgacttcgcc gacaatctcc gcgaggagcg ccgtgagctt
     2041 gttcccctcg atcagcccgg agcgcggaaa ggatatttcg acgaagtagc gggcgatctg
     2101 cttccagtcc agcccgagcg cgacctcggt caggcggtcg agtttccccg ccgcgtacat
     2161 ccctccgacc agcgcgccga tgctcgtccc ggcgacgacg tcgggcgcga gccccgcctc
     2221 ttcgagtgct tgcaggacgc ccaggtgcgc catgccccgc gccgcccccg cgcccagtgc
     2281 gagcccgagg cgcggacgtt tttcggtgtg ctttgaggcc gccgatgggt ttgtcctcgt
     2341 catgatgtct tcgtcacact cctccagaga gccgcaagcg tctccttcgg caggacgacc
     2401 ggattgccct tcatgctgct cgccgccatg gcccctgcga cgacacgctc accgtcagtg
     2461 ccttcgggaa cgtggaacgg ccgcagcggc aggcgcgcag cgaggcgttc gagttcgtcg
     2521 gtcccctctt ccggctcggc gtcgcgtccc gtgagcattt ccgccagctc ggcgtacttc
     2581 ccgagcgccg ggtgcgctcc ctccgggccg agcgcggcga tgttcgcccg gcagacgggc
     2641 gcgagcagcg tggcgcagat cagtccgtgc ggcgcgccgg tcagtcctcc gaccactccc
     2701 gcaaagccgt ggaccgctcc cagccccgcg ttggcgaggg cgacgccgct ccagcacgcg
     2761 gccagcagca tctcctcgcg tgcgccgagg tcgtcggggt cgtcgcacgc cttcggcagc
     2821 gcgcggaagg ccatggggat tcccgcgcgg cagagggcgt cggtccaggc gttcgccttg
     2881 cacgagacga acgcctcgat cagctggcag agcgcgtcca ttccgctcgc ggcgatgacg
     2941 tgggccggca ggccgttcat ggcgtgcggg tcgaggatga cggtctccgg catcatggag
     3001 cggctgcgca ggctcacctt gacctgtttt tcggtgtgcg cgagcaccgc gttgcgggta
     3061 gcctcggatc ctgtccccgc cgtcgccggg atcgccagca gcgggaggga ggttttcatt
     3121 atgggcttcc ccgcgccgac gatctccgcg tagtcgagcg gatcgccgcc gttggtcgcc
     3181 agcatggcca gcgccttgcc ggtgtcgagc acgctcccgc cgcccatggc gaccactgcg
     3241 tcgggggaaa attccgcagc gcgggcggtc gcctgacgaa tactctcaaa gctgggttcg
     3301 cccgacacgg tcagcagctc gcagacgagc ccggccccgc gcagagagtc gagcagttcc
     3361 gccgcccgct caggacggct tccggtgcag acgaggcagc gtttgccgta ctcgcggacg
     3421 atccgccccg cctcccggag acgccctctc ccgaaaagca ccctgcgcgg ctgcgcgcac
     3481 tcgaacggcg tcggtatttc ccggcttccc tgtattttat tcacttgt
//
LOCUS       DAOPKU010000312         3528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29559_length_3528_cov_4.088972, whole genome shotgun sequence.
ACCESSION   DAOPKU010000312 DAOPKU010000000
VERSION     DAOPKU010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29559_length_3528_cov_4.088972"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PK849_09835"
     CDS             <1..691
                     /locus_tag="PK849_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66464.1"
                     /translation="LVAAVGFTVPERFADFLEDGGMSETLGLWENTDELVKGLLAGPC
                     ILSLCGSSKFLVYSLPGVLLQLPERGREGETFAEAFWKKEWSSLVPGVEKLDGFPVGG
                     TTAIPFSIVAAANFRMLQLGLIDRDALRHERFRSIYDYAPLLSDIENAFFWSYLDIPK
                     IASALQDLAKIGQVAAKMGKNLRVSPNALFQAADALKEMGGVTIVMPVPEEGFLEWRI
                     TKDAAPTEEME"
     gene            745..2025
                     /gene="tyrS"
                     /locus_tag="PK849_09840"
     CDS             745..2025
                     /gene="tyrS"
                     /locus_tag="PK849_09840"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPE66465.1"
                     /translation="MFSDALHVLKERGHVEWCSNEEELHSLFTSEMVTAYVGFDPTAD
                     SLHVGHLIPIMALAWIQRCGHRPIPLAGVGTGLIGDPSGKSKERNLLTIEKVQENVIG
                     VKKQLARFLDFDCGGASAQIINNYDWLGKLTFLDVLREVGKHFSVNSMVSREYVKSRL
                     DDPEKSISYTEFSYVLLQAYDFLHLFQNYGCRLQMGGNDQQGNIISGIDLIRKKTGGE
                     AFGATQPLLLTSSGTKFGKTEEGAVWLDPQKTSPYKFYQFWMNTEDQAVEKLLKLFTF
                     LSLEEIEEIMQVHRSAPERREAQRRLAWEVTGIVHGQDNAAMTKKASGILFGESFEPR
                     ELSLSMLDTLAAEVPSGTVSRVFPALLTDILAEAGACSSKSEVRRLLKGGGLSVNGVK
                     VTEEDASVSEDGILAGKYLFVRLGKKRFFMILFQ"
     gene            2055..2930
                     /locus_tag="PK849_09845"
     CDS             2055..2930
                     /locus_tag="PK849_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66466.1"
                     /translation="MDCYWYISDSLRPWEAAVVGTLARALIREGVKLHSFCGGGTESL
                     SIPGLLSWHSLNATERALIVGTKGKLWHLWGTPPSWWKFIRLRSRTIHTLFENPAKDW
                     KGHPTVLSATACSSGESYIPPAFEVKMNWSADEGKESFEETGTPVFLLSGGSAEEFEV
                     FAEGAAKASSTLRNFDESPDEGMKLLSSGNCILLLPRPSSSLALLSAFVALMGIPSVA
                     VRSALMDEMLGKEGYVHLQSENPAEAAHALRSALGEGGRSASAFARRNITDTFSPEKG
                     ARKLKTLYLSLGGDQ"
     gene            2927..>3528
                     /locus_tag="PK849_09850"
     CDS             2927..>3528
                     /locus_tag="PK849_09850"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HPE66467.1"
                     /translation="MRGKAAVLDAIAAFSRPGWRARTVAAFFSGGLKCIRPRNAVARE
                     NLRMVFPEASEEWIRDIVKKLYEHLAWMVAEYLALLREPQQVLEWCEYVEGKAVLDEL
                     KQAGRGAIILTGHVGNWELLAAWLAQSGYPLTAVVRNPDDPNLSEMITAYRTRMGVGS
                     FEKHFVMKEAVRFAKRGGFLGLLPDQAWNATGVMTRFLGH"
BASE COUNT          851 a          846 c          997 g          834 t
ORIGIN      
        1 tctcgtcgct gcagtcggat ttacggtccc cgagaggttt gcggacttcc ttgaggatgg
       61 cggcatgagc gagactttag gtctgtggga aaatacggac gaactggtca aggggttgct
      121 tgcgggtcct tgtattcttt ccttgtgcgg cagcagtaag ttccttgttt attcccttcc
      181 aggagtcctt ctgcagctac cggaacgagg gcgggaagga gaaacatttg cagaggcctt
      241 ctggaaaaaa gaatggagtt cactcgttcc cggggtagaa aagttggatg gattcccggt
      301 aggaggcact acagccatcc ctttttccat agtggccgct gcaaattttc gtatgcttca
      361 gctcggattg atcgacaggg acgcgttacg gcacgagaga tttcgttcga tctatgacta
      421 tgcgccgtta ctttcggaca ttgaaaatgc ttttttctgg tcttatttag atattccgaa
      481 gatagcctcg gcgcttcagg atcttgcaaa aataggtcaa gtagcggcaa aaatgggcaa
      541 gaacttgaga gtgagtccga atgcgctttt tcaagccgcc gatgccctaa aagaaatggg
      601 aggcgtaaca atcgttatgc ctgtccccga agaaggattc ctcgaatgga gaataacaaa
      661 agacgctgca ccaactgagg aaatggagta gaattcacta tcggcggatt gcggttcatt
      721 acagaaaagc gggaggggaa gtacatgttt tcagacgcgc ttcatgttct caaagaaaga
      781 ggacatgtcg aatggtgcag caacgaagaa gagttgcact ctcttttcac aagcgagatg
      841 gtaacggcgt atgtggggtt tgatcctacg gctgacagcc tgcacgttgg ccacctgatt
      901 ccgatcatgg ctcttgcctg gattcaacga tgcggccatc ggccgatccc tcttgcaggg
      961 gttggtaccg gattgattgg agatccttcg ggaaagagca aagaacgaaa tctcctcact
     1021 atcgaaaagg tgcaggaaaa cgtgattgga gtgaaaaaac agcttgctcg cttccttgat
     1081 ttcgactgcg gaggggcatc cgctcaaatt atcaacaact atgactggct agggaagctg
     1141 acgtttctgg atgttctcag agaagtgggt aaacacttct ccgtcaattc catggtctca
     1201 agggagtatg taaaaagcag gctcgacgat ccggagaaga gcatttccta tacggagttt
     1261 tcgtatgttc ttctacaagc ctacgacttc ctgcacctat tccagaacta cgggtgccgt
     1321 ttacagatgg gagggaatga ccagcaggga aatatcatct ccggcattga cctcatccgg
     1381 aagaaaacag gaggagaagc tttcggagca acgcaaccgc tccttctgac ttcatctgga
     1441 acgaaatttg gaaaaactga agagggcgca gtctggcttg atcctcaaaa aacgtccccc
     1501 tacaagtttt accagttctg gatgaatact gaagaccaag ctgtggaaaa gctcctgaag
     1561 cttttcacat tcctctctct cgaagaaata gaggagataa tgcaggtgca ccgaagcgct
     1621 cccgaacgtc gtgaagcgca gcgaaggctg gcctgggaag taaccggcat cgtccatgga
     1681 caggataacg ccgcaatgac gaaaaaagcc agcggaatcc tcttcggaga gtcgttcgaa
     1741 ccccgggagc tttcactctc catgctcgat actcttgctg ccgaagtccc ttcgggaacg
     1801 gtatcccgcg tgttccccgc actgctgacc gatattctcg ccgaagcggg cgcatgttct
     1861 tcgaagagcg aagtacggcg tctcctcaaa ggcggagggc tctccgtcaa cggagtaaaa
     1921 gtaacggagg aagacgcgtc tgtttccgag gacggtattc tcgccgggaa gtacctcttc
     1981 gttcgccttg gaaagaaacg cttcttcatg attctttttc aataggagcc gggtaaaagg
     2041 ggaggggata cctgatggat tgctactggt acataagcga ctctcttcgc ccgtgggaag
     2101 ccgcggttgt cggaactctc gcgagagcgt tgattcggga aggcgtcaag ctccattcat
     2161 tctgcggagg aggaacggag tcgctcagta ttcccggtct actctcgtgg cattcgttga
     2221 acgctacaga gcgtgcgctc attgtcggca cgaagggaaa gctgtggcac ctttggggaa
     2281 ctccgccctc gtggtggaaa ttcatcaggt tacgatcgag gacaatccat acgcttttcg
     2341 aaaatccggc gaaagactgg aaggggcatc ccactgtact ctcggccacg gcatgttctt
     2401 caggagaaag ctatatcccg cctgccttcg aagtgaagat gaactggagc gcagacgagg
     2461 gaaaagaatc ctttgaagaa accgggaccc ctgtttttct tctctcggga ggtagcgccg
     2521 aagagtttga ggtgtttgca gaaggagcgg cgaaagcgtc cagcactctc cgaaactttg
     2581 acgaatcgcc ggatgaaggg atgaaactcc tctcctccgg caactgcatt ctgctccttc
     2641 cacgcccctc atcttcgctt gctctgcttt cggctttcgt cgctttgatg gggatcccct
     2701 cggtagccgt tcgctccgca ctgatggacg agatgcttgg gaaagagggg tacgttcatc
     2761 tgcagtcgga gaatcccgca gaagccgcgc atgctcttcg atcggcattg ggcgaaggag
     2821 gcaggagcgc ttccgctttt gcgcggcgca acattacgga cactttctcc cctgaaaaag
     2881 gagcaaggaa acttaagacc ctatatctct ctcttggagg agatcagtga gaggaaaggc
     2941 agccgtcctc gacgccattg cagcttttag ccgtccgggg tggcgcgctc gtaccgtcgc
     3001 cgccttcttt tcgggcggat tgaaatgtat ccgcccccga aacgctgttg ccagggagaa
     3061 tctgcgtatg gtgtttccgg aagcctcgga ggagtggatt cgggatatcg taaaaaaact
     3121 gtacgagcat ttggcgtgga tggtggcgga gtaccttgct cttcttcggg agcctcaaca
     3181 agtgcttgaa tggtgcgaat acgtcgaggg caaagcagtt ctcgatgagc tgaagcaggc
     3241 tgggagggga gcaattatcc tgaccggtca tgtagggaat tgggaactcc tcgccgcttg
     3301 gctggcacaa tcaggatatc cgcttactgc cgttgttcgc aatccggatg atccgaatct
     3361 ttcagagatg atcacagcgt atcggactcg aatgggtgtt ggctcttttg aaaaacactt
     3421 tgttatgaag gaagctgtcc gatttgcaaa aagaggtgga tttttaggat tgctgcccga
     3481 tcaggcatgg aacgcaaccg gagtgatgac gcgtttcctg ggacataa
//
LOCUS       DAOPKU010000313         3522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29650_length_3522_cov_4.702913, whole genome shotgun sequence.
ACCESSION   DAOPKU010000313 DAOPKU010000000
VERSION     DAOPKU010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29650_length_3522_cov_4.702913"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="PK849_09855"
     CDS             <1..2066
                     /locus_tag="PK849_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66468.1"
                     /translation="QTPPKGGGYKSFYEDIEKEASKGKVKNFVSREEETTIGGQPAVF
                     YSFSGEMEVGSGSRKMEGNMVLSVQPDAEGKHTLVALMGTSSSLEQLRDEIKSILASA
                     REARAPLRPLASFPYGSTQESFRHIYGPAVAADGAIAVAETLGEGKVRIFDSKGAQLA
                     EWGSRGKGEDGTFNYPQAMVFGPDGSLYVVDGGYTVDPHVQRFSRTGEFLGKIRADKK
                     SRGDDGIYTPSFLAVTDSGKIVIAGLTEISGGVPRVVVFSPEGDVLSKWEPGEIGGIV
                     ALPGDRLLVTKPHPENDRNPLFTVLDLEGKTLKEWPFFGSDLPSTPGDEKTYFRPKFI
                     TADAQGRVYAYDDSEDAVWMYDAEGKFMYALPVRPPLGIVGGMAASPRGDLIFHDRPS
                     GYGPGEPSIHLFENAFPASAPALPAPEVAPETTEKPPVASEAAEESTPLEVELARLKK
                     ALALREEAERLENEGDLAGAAVKYKESLPLHQDPEVETYAADLEERATLASMVDTLPV
                     PTGVPEKEEPPVQEPPKVVRSPLYNEAEALESSGKRYEALQKYREALTSQHDEKMKEH
                     ADALEKTLEGEARKVVERAVAVQKEGKLDEALALYRESLGIYPVERIREYAERLDALI
                     QSRAQGGDARSRAETIWRQAAELQKAEKYEEALVKYREGLAIFHNAAVEEHIVKLEKF
                     IQKRK"
     gene            2319..2579
                     /locus_tag="PK849_09860"
     CDS             2319..2579
                     /locus_tag="PK849_09860"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HPE66469.1"
                     /translation="MKYLFYLLLIVNLFGFLIMWWDKRCARKGARRVPERTLFLVALC
                     GGAVGGLGGMYLFRHKTQHWRFTIGFPLILVVQIAAVAYFMV"
     gene            2668..3423
                     /locus_tag="PK849_09865"
     CDS             2668..3423
                     /locus_tag="PK849_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE66470.1"
                     /translation="MKLLVIALLAIVVSGASLSCAAEAAQSFEEDIFQTSAGEVKITF
                     VGHGTLIFSFDGKVLHVDPYSKVADYSLLPKADGVLITHEHQDHLDKAALQHILTPET
                     DIVLNAKSAEILGRGRVLQNGHATRLLGIPVEAVPAYNIVHKRDTGQPFHPKGEHNGY
                     ILTFGDTRIYIAGDTENTPEMKALQGIDAAFLPMNLPFTMTPEMVADAALAFKPKALY
                     PYHMGQMDEERPAKLAELLKGSGIDLRIRKMGR"
BASE COUNT          766 a          971 c         1121 g          664 t
ORIGIN      
        1 aacagacgcc ccctaaaggg ggagggtata agagctttta cgaggacatc gaaaaagagg
       61 cgtcaaaggg gaaggtgaag aacttcgtct cgcgggagga ggaaacgacg attggggggc
      121 aacccgccgt attctactct ttctccggag agatggaggt agggagcggt tccaggaaga
      181 tggaaggaaa catggtcctg agcgtccagc ccgacgccga ggggaagcat acgctcgttg
      241 cgctgatggg gacctcttcc tctctggagc aattacggga cgagatcaag agtatcctgg
      301 cgtcggccag ggaggcgaga gcgccgcttc gccccctcgc ctctttcccg tacggttcca
      361 cccaggagag cttccggcat atctacgggc ccgccgtggc cgcggacggc gcgatcgccg
      421 tcgctgaaac ccttggagag ggaaaggtgc gcatcttcga ctcgaaagga gcgcagctcg
      481 ccgaatgggg gagcaggggg aagggagaag acggaacctt caactatccg caggccatgg
      541 ttttcggccc cgacggttcg ctctacgtcg tcgacggcgg ctacactgtc gacccccatg
      601 tgcagcggtt ttcccgcaca ggagagtttc tgggcaaaat acgcgccgac aaaaaatcca
      661 gaggcgacga cggaatatac actccttcat tcctggcggt cacggattcg ggaaagattg
      721 tgatcgcagg cctcaccgag atcagcggcg gcgtgccgcg cgtcgttgtt ttttctcccg
      781 aaggggacgt gctctcgaag tgggaaccgg gagagatcgg aggaatcgtc gcgcttcctg
      841 gagatcgtct gcttgtgacg aagccgcatc ctgagaacga caggaacccc ctcttcacgg
      901 tgcttgacct tgaggggaaa acgctgaagg agtggccgtt cttcggaagc gaccttcctt
      961 cgacgccagg cgacgagaaa acctacttca ggccgaagtt cataaccgcc gacgcacagg
     1021 gcagagtgta cgcctacgac gattcggaag acgcggtctg gatgtacgac gccgaaggga
     1081 agttcatgta cgcccttccc gtaagaccgc cgctgggtat cgtcggcggg atggcggctt
     1141 cgccgcgcgg cgacttgatc ttccacgacc gtccgagcgg ctacggcccc ggcgagccgt
     1201 cgattcatct gttcgagaat gccttccccg cgtcggcgcc cgctcttcct gcgcccgagg
     1261 tcgcgccgga aaccacggag aaacctcccg tcgcgtcgga ggcggcggag gagagcactc
     1321 cgcttgaggt cgagctggcc aggctgaaga aagcgctcgc gcttcgggaa gaagcggaac
     1381 gtcttgaaaa cgagggcgat cttgccggag cggcggtgaa gtacaaggag agcctgccgc
     1441 tccatcagga tccggaggtg gagacgtatg ccgccgacct tgaggagcgg gcgacgcttg
     1501 cctcgatggt ggataccctc cccgtgccga ccggtgtccc ggagaaggag gaaccgcccg
     1561 tgcaggagcc gccgaaggtc gttcgttcac cgctctataa cgaggcggag gcgcttgaaa
     1621 gctcggggaa acggtacgag gcgcttcaga agtaccggga agccctcact tcacagcatg
     1681 acgagaaaat gaaggaacat gccgacgctc tggaaaaaac gctcgaagga gaggcgcgca
     1741 aggtcgtcga acgggcagtc gccgtgcaaa aagaaggaaa actcgacgag gcgcttgccc
     1801 tgtacaggga aagcctgggt atttacccgg tggagcgcat acgcgaatac gccgaacgcc
     1861 tcgacgcgct catccaaagc cgcgcacagg gaggcgacgc acgcagccgc gccgagacga
     1921 tctggcggca ggcggcggaa cttcagaagg ccgagaagta cgaggaggcg ctcgtgaaat
     1981 accgcgaggg actcgccata ttccacaacg cggcggtgga ggagcacatc gtgaagctgg
     2041 agaagttcat acagaagagg aagtaacccg ccgtgcagac agattctttt tgaagcggcc
     2101 tctgcgtttt gccggggagc gaaggtctgg cgcgtcatcc ggagtctgcg gaagacgcgc
     2161 cagttttctg cgagacgttt tcgaaaagtc gtcgtacaat atacgaacac ggatggcaac
     2221 tatccgtcgt ccgcatgcca atcctatttg gagagctccg ttattcatcc gagggggcgg
     2281 tcttcgtgga cacaatgatt tctgaaaggg gtctgtgaat gaaatacctg ttctatcttt
     2341 tactgatcgt caatctcttc ggctttctga ttatgtggtg ggacaaacgg tgtgcgcgga
     2401 agggcgcgcg ccgggttccg gaaagaacgc tttttctggt tgccctatgc gggggagcgg
     2461 ttggaggtct gggagggatg tatctcttcc ggcacaagac ccagcattgg cgttttacga
     2521 taggctttcc tctgatcctt gtggtacaga ttgcggcggt tgcttatttt atggtataac
     2581 cgaaagaatc gctcttcttg ccgaggcggc ggcgtacaag tccgtgtata atactcccaa
     2641 ctttgcaaag aggaggagtg atcgcttatg aaattgctcg tcatcgcgct gcttgcgatt
     2701 gtcgtttccg gagcgtcctt aagctgcgcc gcggaagctg cgcagtcgtt cgaggaggat
     2761 attttccaga cctccgccgg tgaggtgaaa atcactttcg tcggtcatgg cacgctgatt
     2821 ttcagttttg acggcaaggt gctccacgtc gacccttaca gcaaggtggc cgactactcg
     2881 ttgcttccga aagcggacgg cgtgctcatt acgcatgaac atcaggatca cctggacaag
     2941 gctgcgctgc agcatattct gacgccagag accgatatcg tgctcaacgc gaagagcgcg
     3001 gagatcctcg gacgcggccg ggtgctgcag aacggccacg caacacggct tttgggaatt
     3061 ccggtcgaag ctgttcccgc ctataacatc gtccacaaga gggacaccgg gcagccgttc
     3121 cacccgaagg gcgagcacaa cgggtacatc ctcacgttcg gggatacccg gatctacatc
     3181 gccggagaca ctgaaaacac gccggagatg aaagcgctgc agggtatcga cgccgctttt
     3241 cttccgatga accttccctt caccatgacg ccggagatgg ttgccgacgc tgccctcgcc
     3301 ttcaaaccga aggcgctcta cccctaccat atggggcaga tggacgagga gcgtccggcg
     3361 aagctggcgg aactgctcaa gggaagcggt atcgacctga ggatccgaaa gatggggcgg
     3421 tagcggaaaa gaatccggac tgtcggcaag gcgggggggc ggcgcctctc cgccttgcgt
     3481 tgtctttcgc gttctttatt tggccgcaat aaaccgccac tc
//
LOCUS       DAOPKU010000314         3499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_29959_length_3499_cov_5.433798, whole genome shotgun sequence.
ACCESSION   DAOPKU010000314 DAOPKU010000000
VERSION     DAOPKU010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29959_length_3499_cov_5.433798"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PK849_09870"
     CDS             complement(<1..478)
                     /locus_tag="PK849_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66471.1"
                     /translation="MNASLMPELLERIQKAFRPIEVRPGAPLPKLPGALPYVKELSGY
                     PRFCGSTMGAEILWNMEILEKAASKPNDPDFTAEYTVAGRHTRVLGRNHRYLDYPVNV
                     FLNICMLHDGQVEIFDFCLNLLAEIAPVNWYAQTLPEEMRGCAIGGKDAYAFDRKNE"
     gene            728..1921
                     /locus_tag="PK849_09875"
     CDS             728..1921
                     /locus_tag="PK849_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HPE66472.1"
                     /translation="MKKAYVVGTCDTKFEELKFVADLIRQSGVEALLVDVGTKPHEHS
                     VDITSREVAACHPSAPSFLDTVKERGEAVGAMGEALAAFLPGRGDVGGVVGLGGSGGT
                     SIVTSGMRALPLGMPKIMVSTMASGNVAPYVGPNDIAMMYSVTDVAGINKVSRVVLAN
                     AANALAGMVKGTVPAGEDRPLLGMTMFGVTTPCVDMVRHALESEFDCLVFHATGAGGQ
                     SFEKLVDSGMMKRVLDVTTTEICDHLMGGVLSAGEDRLGAFARTGISYVGSVGALDMV
                     NFGAMETVPEKYKNRKLYVHNPQVTLMRTTPEENTLMGEWIGKKLNAMTGPVRFLLPL
                     KGVSMIDAPGQAFYWPEADEALFAALERTVQQSDTRRLVKLPLHVNDPEFAAALVENY
                     REINP"
     gene            1941..2771
                     /locus_tag="PK849_09880"
     CDS             1941..2771
                     /locus_tag="PK849_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HPE66473.1"
                     /translation="MKRPTRSELMAKFHDMAKRREPIIGGGAGTGISAKWEEAGGIDL
                     IVIYNSGRYRMAGRGSLAGLLAYGNANDIVKEMAYEVLPVVKKTPVLAGVNGTDPFTI
                     WDKYFDELISLGFAGVQNFPTVGLIDGVFRANLEETGMGYGLEVEAMKAAHERDLLVT
                     PYVFSEEDAVAMAEAGADILVCHMGLTTKGSIGAKTAKTLEECAPLIDSWAEAAKKVR
                     PDILVLCHGGPIAMPEDASYILKHCKHVHGFYGASSMERLPTETAIKAQTQDFKKITF
                     "
     gene            2785..3327
                     /locus_tag="PK849_09885"
     CDS             2785..3327
                     /locus_tag="PK849_09885"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPE66474.1"
                     /translation="MPRVYYSTILSATVEEAWEVVRDFNGLPKWHPGITASELEDACS
                     PDCEACIGSVRHFQLANGEWMREQLLSLSDLDFSVSYTILETGMKLKNYVATLKLTPV
                     TATDETFAEWEAVFDTTDPASEEETAEIVHGVFAEGLENLDDILLEAYEEESGDGCGG
                     CGSGGCGCGSCSCSGDEEEE"
BASE COUNT          732 a         1043 c         1100 g          624 t
ORIGIN      
        1 tctcgttctt tctgtcaaag gcataggcat cctttcctcc gatagcgcac ccccgcatct
       61 cctccgggag cgtctgggcg taccagttga caggagctat ttcggcaagg agatttaaac
      121 aaaaatcgaa tatctcgacc tgaccgtcgt ggagcatgca gatgttcagg aagacgttga
      181 cagggtagtc caggtagcgg tggttccgtc cgagcactct ggtatgcctt cctgcgacgg
      241 tgtactcggc ggtgaagtcc ggatcgttcg gttttgaagc cgccttctcc agaatttcca
      301 tattccagag aatctcggcg cccatggtgg agccgcagaa tctcgggtag ccgcttagct
      361 ccttgacgta gggaagcgcg ccgggaagct tgggaagagg cgctcccgga cggacctcta
      421 tcggacgaaa ggctttctgt attctttcaa gcagttccgg catgagactg gcgttcatga
      481 cgacacctcc tgcaaagcaa gataggcaca gactcttttc tttattctag cagtacaccg
      541 aaagaataac cagagagtta ccaaaatgtg tactgcatcc gatagcgcat cgtgaaaaga
      601 gttccggtgc gcagctcgat caccccggaa gcgggaattt tcacacagca gggggctgtt
      661 aagggtataa tgtccctgtg gtttttcccc ggacaggggt atcctgtgat gcagcaagga
      721 ggacggaatg aagaaagcct atgttgtagg aacgtgcgac acgaagttcg aggagctgaa
      781 gttcgtggcc gatctcattc ggcaatcggg cgtcgaggcg ctcctcgtgg acgtggggac
      841 caagccccac gagcactctg tagacatcac gagccgcgaa gtggccgcgt gccacccgtc
      901 ggccccctcc ttcctcgaca ccgtgaagga gcgaggcgag gcggtcggcg ccatgggcga
      961 ggcgctcgcc gcgtttctcc cgggccgggg cgacgtcggc ggtgtcgtcg ggctgggagg
     1021 ctcaggcggg acatccatcg tcacctcggg catgagggcg ctgcccttgg ggatgccgaa
     1081 gatcatggtc tccaccatgg cgtcgggaaa cgttgccccc tacgtcgggc cgaacgacat
     1141 cgccatgatg tactccgtga ctgacgtcgc ggggatcaat aaggtctcgc gcgtcgttct
     1201 ggcgaacgcg gccaacgccc tcgccggcat ggtgaaggga acggttccag cgggagagga
     1261 caggccgctc ctgggtatga cgatgttcgg ggtcaccaca ccgtgcgtgg acatggtccg
     1321 tcatgcgctc gaaagcgagt tcgactgcct cgtcttccac gcgaccggag cgggcggaca
     1381 gtccttcgag aagctggtgg actccgggat gatgaagcgc gtcctcgacg tcaccaccac
     1441 cgagatctgc gaccacctga tgggcggcgt tctcagcgca ggggaggacc gcctcggggc
     1501 cttcgcccgg acaggaatct cctacgtcgg ctcggtcggc gctctcgaca tggtgaactt
     1561 cggcgcgatg gagaccgtac cggagaagta caaaaaccgc aagctctacg tccacaaccc
     1621 gcaggttacg ctgatgcgca ccacgcccga agagaataca ctgatgggcg agtggatcgg
     1681 gaagaagctg aacgccatga cggggccggt gcgcttcctg ctcccgctga agggcgtttc
     1741 gatgatcgac gcgcccgggc aggcgttcta ctggccggag gcggacgagg cgctttttgc
     1801 ggcgctcgag cgtaccgtgc agcagagcga tacgcgccgc ctcgtgaagc tccccctgca
     1861 cgtcaacgat ccggagttcg ccgcggcgct cgtggagaac taccgggaga tcaatccgta
     1921 acgaaaggaa ggattcactg atgaaacgac cgacacgctc ggagcttatg gcgaagttcc
     1981 acgacatggc gaagcggcgc gagccgatta tcggcggcgg cgcgggaacg ggcatctccg
     2041 cgaagtggga ggaagcggga ggcatcgacc ttatcgtcat ctataactcg ggccgctacc
     2101 gcatggccgg gcgcggctcg ctcgccggac tcctcgccta cgggaacgcg aacgacatcg
     2161 tcaaggagat ggcgtacgaa gtccttcccg tggtgaagaa gacgccagtc ctcgccggag
     2221 tcaacggcac agaccccttc accatctggg acaagtactt cgacgagctg atctccctcg
     2281 gattcgccgg agtgcaaaac ttcccgaccg tcggtctcat cgacggcgtc ttccgggcca
     2341 acctggagga gaccggcatg gggtacggcc tcgaagtgga ggccatgaag gccgcgcacg
     2401 agcgagatct cctcgtcacc ccgtacgtct tcagcgagga ggacgccgtc gccatggccg
     2461 aggcgggagc cgacatcctc gtctgccaca tggggctgac cacgaagggc tccatcggcg
     2521 cgaagaccgc aaagacgctc gaggagtgcg cccccctcat cgacagctgg gccgaggccg
     2581 cgaagaaggt ccgcccggac atactcgtcc tctgccacgg cggcccgatc gccatgcccg
     2641 aggacgcctc gtacattctg aagcactgca agcacgtcca cggcttctac ggcgcaagct
     2701 ccatggagcg gctgccgacg gagactgcca taaaggccca gacgcaagat ttcaagaaga
     2761 tcacattctg aggaggaaac gaatatgccg cgagtgtact acagcaccat actctccgcc
     2821 accgtagagg aggcgtggga ggttgtccgc gacttcaacg gcctgccgaa atggcatccg
     2881 ggaatcacgg cgagcgaact tgaggacgca tgcagccccg actgcgaggc gtgcatagga
     2941 agcgtgcgcc acttccagct cgcgaacggc gagtggatgc gcgaacagct tttgtccctt
     3001 tccgacctgg acttctcggt cagctacaca atccttgaga ccggaatgaa gctgaagaac
     3061 tacgtcgcca ccctgaagct cacgccggtc accgcaaccg acgaaacctt cgccgagtgg
     3121 gaggccgtct tcgacaccac cgaccccgcc agcgaggaag agacggcgga gatagtccac
     3181 ggcgtcttcg ccgaaggcct tgaaaacctc gacgacatac tcctggaggc ctacgaggag
     3241 gaatccgggg acggatgcgg ggggtgcgga agcggcggat gcggctgcgg cagttgcagc
     3301 tgcagcggag acgaagaaga ggagtagttg ttccctattg ccaatgcgtt cataaccggg
     3361 atcccttgta cctcagggga tcccggttca tgttccgatt cctccaaccg ccttctcggc
     3421 cgcagggtta ctttttccgc tcgaaaaact gcacgttcag tccgttcgga tccttgatgc
     3481 agaaaaactt gaaagcggg
//
LOCUS       DAOPKU010000315         3490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_30068_length_3490_cov_5.478020, whole genome shotgun sequence.
ACCESSION   DAOPKU010000315 DAOPKU010000000
VERSION     DAOPKU010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30068_length_3490_cov_5.478020"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /gene="gcvPB"
                     /locus_tag="PK849_09890"
     CDS             complement(<1..1360)
                     /gene="gcvPB"
                     /locus_tag="PK849_09890"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPE66475.1"
                     /translation="MKRTMDTLFETSRPGRVGYALPELDVPAVDCGKELKGFCRAAAP
                     RLPELAEVDVVRHFTGLSKMNYSVDEGFYPLGSCTMKYNPKVNEVAARMPGFSRIHPL
                     QPEGTVQGALKLMYDLSAMLAEITGMDAVTLQPAAGAHGEQTGLMMIKAYHESRGDHK
                     RTKVIVPDSAHGTNPASAAVTGFDTVEVASDAEGMVDLDALKALMDDSVAALMLTNPN
                     TLGVFERNILALTETIHAAGGLVYYDGANANAIMGKARPGDMGFDVLHLNLHKTFSTP
                     HGGGGPGAGAVGVKDVLVPFLPTPVIGFADGAYRLEHADMHPKSIGRVRSFFGNFGVL
                     VRAYTYIRTLGAEGLQEVSETAVLNANYLAKKLEHIYPLAHKGYCMHEFVLNGGALKH
                     ETGVSSLDVAKALIDAGYHPPTVYFPLIVPEALMVEPTETESQETLDRFAAAMEEIVA
                     KAK"
     gene            complement(1357..2709)
                     /gene="gcvPA"
                     /locus_tag="PK849_09895"
     CDS             complement(1357..2709)
                     /gene="gcvPA"
                     /locus_tag="PK849_09895"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPE66476.1"
                     /translation="MNRYIPVTAEDRRRMLGVIGAASVDDLFSGIPVQVRQKKASDLP
                     KALSEPEMLRDLRALSGKNLNADDAICFLGAGVYDHFIPSAVDSIVSRGEFTTSYTPY
                     QPEVAQGTLQAIFQYQTMVCELTGMEISNASMYDGASAAAEAAVLAASATKKRTVLCG
                     RNVHPQTRQTVRTYCWSRRLEYAELPAENGRIDRAALAKALEGGDAAALIVQSPNFFG
                     NLENLQELGDAVHEAKGLFIVSSDLLALGTLEAPGKLGADIVVGDGQTAGNPMNFGGP
                     AFGFIAVNKPLMRKMPGRICGQTTDSQGRRTFVLTLQAREQHIRREKATSNICSNQNL
                     CIVAASVYLSLMGPQGLRDIGGQILAKSAYAVEQLEKTGKFRRAFPGVPSFRETALLC
                     EEPVDKLNARLLQQGILGGYSLEAEYPRLKNGWLLAVTESRTKEEIDRLVAVAGGDAR
                     "
     gene            complement(2722..3120)
                     /gene="gcvH"
                     /locus_tag="PK849_09900"
     CDS             complement(2722..3120)
                     /gene="gcvH"
                     /locus_tag="PK849_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPE66477.1"
                     /translation="MAKIPQDLHYTKDHEWIKIVDGMGVMGISDYAQHAMGDVVYVEL
                     PDVGRKAKAGEDFIVVESVKGANDVFCPVSGTVAGVNEDLDGAPELLNQDPYSNWLVK
                     MEVDDPSDVDKLLDAAAYAKLVEELEAEES"
     gene            complement(3156..>3490)
                     /locus_tag="PK849_09905"
     CDS             complement(3156..>3490)
                     /locus_tag="PK849_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HPE66478.1"
                     /translation="FVKVDKAGGFIGQPVLKRQKEEGLKHRTVALRLEDKGVPRHGMD
                     VANAEGRVVGHVTTGGYSPSLDANIASCYVETPAPAEGEHLWIVIRDKPKQGVVVKRP
                     FYAKSYKS"
BASE COUNT          513 a         1122 c         1160 g          695 t
ORIGIN      
        1 ccttcgcctt cgccacgatc tcctccatgg ccgccgcgaa tcggtcgagc gtctcctgcg
       61 actccgtctc cgtgggctcg accatcagcg cctccggaac gatgagcggg aagtagaccg
      121 tcggcggatg gtaccccgcg tcgatcagcg ccttggccac gtcgaggctg cttactccgg
      181 tctcgtgctt cagcgcgcct ccgttgagga cgaactcgtg catgcagtac cccttgtgcg
      241 cgagcgggta gatgtgctcg agctttttgg cgaggtagtt cgcgttgagc accgccgtct
      301 cggagacctc ttgcaacccc tctgccccca aagttctgat gtaggtgtag gcgcggacga
      361 gcacgccgaa gttgccgaag aagctgcgca ccctgccgat ggacttcggg tgcatgtccg
      421 catgttccag ccggtacgct ccgtccgcga agccgatcac cggcgtcgga aggaacggga
      481 cgagcacgtc cttgacgccg accgcgcccg ctccggggcc gcccccgccg tgcggcgtgc
      541 tgaaggtctt gtggaggttc agatggagca cgtcgaaccc catgtcgccg gggcgcgcct
      601 tgcccatgat ggcgttcgcg ttggccccgt cgtagtagac gagccctccc gcggcgtgga
      661 tggtctccgt cagggcgagg atgttccgct cgaagacgcc gagcgtgttc gggttggtga
      721 gcatgagcgc ggcgacggag tcgtccatca gcgccttgag cgcgtcgagg tcgaccattc
      781 cctcggcgtc tgaggcgacc tcgaccgtat cgaagccggt gaccgccgcc gacgccgggt
      841 tggtgccgtg cgcggagtcg gggacgatga ccttcgtccg cttgtgatct ccccggctct
      901 cgtggtacgc cttgatcatc atcaggccgg tctgttcgcc gtgggctccg gcggcgggct
      961 ggagcgtgac cgcgtccatc cccgtgatct ccgcgagcat cgcgctcagg tcgtacatga
     1021 gcttcagcgc gccctggacc gttccctcgg gctgaagggg gtggatgcgc gagaagcccg
     1081 gcatccgggc ggcgacctcg ttcaccttcg ggttgtactt catggtgcac gagccgagag
     1141 ggtagaaccc ctcgtcgacc gagtagttca tcttcgagag gcctgtgaag tggcgcacca
     1201 cgtccacctc ggccagttct ggcagtctgg gggccgcggc gcggcagaag cccttcagct
     1261 ccttgccgca gtccacggcg ggaacgtcga gttccggcag cgcgtagccg acccgtccgg
     1321 ggcggctggt ttcaaaaagc gtgtccatcg tgcgtttcat cgggcgtcac ctcccgcaac
     1381 cgcgacgagg cggtcgatct cttccttcgt gcggctctcc gtcaccgcga ggagccagcc
     1441 gttcttcaag cgcggatatt ccgcttccag ggagtacccg ccgagaatgc cctgctgcag
     1501 aaggcgggcg ttcagcttgt ccaccggttc ctcgcagagg agcgcggttt cgcggaaaga
     1561 cggaacgccg gggaacgccc ttcggaattt gcccgtcttt tcgagctgct ccaccgcgta
     1621 ggcggacttg gcgagaatct ggccgccgat gtcgcggagt ccctgcggcc ccatgaggct
     1681 gaggtagacc gaggccgcga cgatgcagag gttctggttc gagcagatgt tcgacgtggc
     1741 cttctcgcgg cggatgtgct gctcgcgcgc ctggagcgtg aggacgaagg tgcggcgtcc
     1801 ttggctgtcg gtggtctggc cgcagatgcg tcccggcatc ttccgcatca ggggcttgtt
     1861 cacggcgatg aagccgaagg cggggccgcc gaagttcatc gggttgcccg ccgtctggcc
     1921 gtcgccgacg acaatgtcgg ccccgagctt tccgggcgct tcgagggttc ccagcgcgag
     1981 caggtcggag gagacgatga agagcccctt cgcctcgtga acggcgtctc cgagttcctg
     2041 caggttctcc aggttgccga agaagttggg cgactggacg atcagcgcgg cggcgtcccc
     2101 gccttcgagc gccttcgcca gcgctgcgcg gtcgatccgg ccgttctccg cggggagttc
     2161 ggcgtactcg agtcgccggc tccagcagta ggtacggacg gtctgcctcg tctgcggatg
     2221 cacattccgg ccgcagagta ccgtacgctt ttttgtcgcg gaggccgcaa gcaccgcggc
     2281 ttcggcggcg gcggaggcgc cgtcgtacat ggaggcgttg gagatctcca tgccggtgag
     2341 ttcgcagacc atggtctggt actggaagat ggcctggagc gtcccctgcg ccacctccgg
     2401 ctggtagggg gtgtagctgg tggtgaattc gccccgcgag acgatgctgt cgaccgccga
     2461 ggggatgaag tggtcgtaga cccccgcgcc gaggaagcag attgcgtcgt ccgcgttcag
     2521 attttttcct gagagagcgc gaagatcgcg gagcatctca ggctctgaga gggccttcgg
     2581 cagatcggag gccttcttct gacggacctg cacggggatt ccggagaaga ggtcgtcaac
     2641 gctcgcggcg ccgatgacgc cgagcatccg gcgacgatcc tccgccgtga cgggaatata
     2701 acggttcatg gcgatccccc cttacgactc ttccgcttcc agctcctcga cgagcttggc
     2761 gtacgcggcg gcgtcgagga gtttgtccac gtcggaggga tcgtccacct ccatcttgac
     2821 cagccagttg gagtacggat cctggttcag cagctcgggc gcgccgtcga gatcctcgtt
     2881 gaccccggcg accgtgccgg agacggggca aaagacgtcg ttcgcgccct tcaccgactc
     2941 cacgacgatg aagtcctcgc cggccttcgc ctttctgccg acgtcgggaa gctccacgta
     3001 gaccacgtcg cccatcgcgt gctgcgcgta gtcgctgatg cccatgacgc ccatgccgtc
     3061 gacgattttg atccactcgt gatcctttgt gtagtgaaga tcctggggta tttttgccat
     3121 gctgttcatc ctcctcttgt ttttggtttc agttttcagc tcttgtagct ctttgcgtag
     3181 aacgggcgct tgaccacgac gccctgtttc ggcttgtcgc ggatgacgat ccagagatgc
     3241 tctccctcgg cgggcgcggg ggtctccacg tagcacgagg cgatgttggc gtccagcgag
     3301 gggctgtacc cgcccgtcgt cacgtgcccg acgacgcgcc cctcggcgtt cgcaacgtcc
     3361 atgccgtgcc ggggaacgcc cttgtcctcc aggcgcagcg ccacggtgcg gtgtttcaat
     3421 ccctcttcct tctggcgttt gaggacgggc tgtccgatga agccgcccgc cttgtccacc
     3481 ttcacgaaga
//
LOCUS       DAOPKU010000316         3489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_30081_length_3489_cov_4.365754, whole genome shotgun sequence.
ACCESSION   DAOPKU010000316 DAOPKU010000000
VERSION     DAOPKU010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30081_length_3489_cov_4.365754"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..837)
                     /locus_tag="PK849_09910"
     CDS             complement(166..837)
                     /locus_tag="PK849_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE66479.1"
                     /translation="MNEEMTLESLAARNAGDSSAPHYIAGVLREAIYRGLLPEGKPLH
                     QAQLALRLGVSPIPLREALRLLETEGLIAFQGYRGAMVTALSLEEARELYEMISALET
                     SLMKIAFPRITRRVVEDAAKVLDLMEREPDCIKWRDLNQMFHNLFYEPADRPLTLDML
                     ARLRQKTDRNIRLHLASMRDESQRQHRAILDAVAARTLDAALEALAAHLAYTSNDLQS
                     CMRRG"
     gene            979..2166
                     /locus_tag="PK849_09915"
     CDS             979..2166
                     /locus_tag="PK849_09915"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPE66480.1"
                     /translation="MAVPMKFLSEELETMKQGGFYGTIRTLESPQGAWVHIDGKKYLN
                     LCSNNYLGLCNDPRLVNKVKEYADKYGVGPGAVRTIAGTMSPHIELEQKLAAFKGAEA
                     AIVVQSGFCANLAVIPTLASSADDVIFSDSLNHASIIDACKLTKAKVVRYEHSDMADL
                     EKKLKEYDGFAGKKLLITDGVFSMDGDVAKLPEIVELCEKHGVIVAVDDAHGEGVLGR
                     GGRGIVDHFGLHGRVDVEIGTMSKAFGVMGGMAAGSAILVDYLRQKARTNLFSSALTI
                     PDVAANLASVEILQQSEDLVKKLWHNGDFLKRELKALGFDTAHSETPITPVIIGEASD
                     AKAFSAKLFEAGVFATAIVFPTVPRGTARIRAMVSAAHSEDDLRFAVEKFAAVGKAMG
                     IIK"
     gene            2163..3107
                     /locus_tag="PK849_09920"
     CDS             2163..3107
                     /locus_tag="PK849_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPE66481.1"
                     /translation="MKKILVTGANGQIGVELVVHLRKLYGAENVLATDLRKTPGAASE
                     GGPFAALDVRDPKAFSETLRSFGADTVLHLAGILSARGEADPMFSWDVNVNGTCAALE
                     AAREHGAAFFFPSSIAAFGPSTPSKNTPQDTLQRPSTVYGVAKVSMELLCDYYHKKFG
                     MDTRGLRFPGLISYEALPGGGTTDYAVHIYYDAIKKGSYTSFIAQGTYMDMMYMPDAL
                     NAIVRLMEADPARLLHRNAFNISAMSFEPEQIAASIRKFMPGFTMDYDVDPLRQGIAE
                     SWPDSLDCSAAREEWGFAPEYDLDRMSEDMLARLKEKL"
     gene            3190..>3489
                     /locus_tag="PK849_09925"
     CDS             3190..>3489
                     /locus_tag="PK849_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPE66482.1"
                     /translation="MLDKALPSVLGAIGKTPLVRLDRIARAWNLEGTLYAKLEYLNPG
                     FSKKDRIALQMIEEAEERGVLHPGQTVVELTSGNTGTGLAIVCGVKGYPFCAVMSK"
BASE COUNT          652 a         1016 c         1219 g          602 t
ORIGIN      
        1 aatgtgagca acggaagcat aatggactgg aagtcgggaa tgggcatctg aaaacctcgc
       61 tttcaatcgg attgaatcgc cacaagagag cggatgtcca atctataccg ctcttgatga
      121 agactgtcaa tctccgctct ttagcttcct cacgctcctc ccccctcacc cccgccgcat
      181 gcacgactgc aggtcgttcg acgtgtacgc caggtgcgcg gccagggcct ccagcgcggc
      241 gtcgagggtg cgcgccgcga cggcgtcgag gatggctcgg tgctgccgct gcgactcgtc
      301 cctcatggag gcgaggtgga ggcggatgtt gcggtcggtc ttctggcgca ggcgggcgag
      361 catgtcgagg gtcagggggc ggtcggcggg ctcgtagaag aggttgtgga acatctggtt
      421 gaggtcgcgc cacttgatgc agtcgggctc gcgctccatc aggtcgagca ccttggcggc
      481 gtcctcgacg acgcggcggg tgatgcgggg gaaggcgatc ttcatcaggc tggtctccag
      541 cgcggagatc atctcgtaca gctcgcgggc ctcctccagt gagagcgcgg tgaccatggc
      601 tccccggtac ccctggaagg cgatcaaccc ctccgtctcc agcaggcgca gtgcctcgcg
      661 caaggggatg gggcttacgc cgaggcggag ggcgagctgg gcctggtgca gcggtttgcc
      721 ctcgggaagg agcccgcggt agatggcctc ccgcagcact cccgcgatgt aatggggggc
      781 ggagctgtct cccgcgttgc gcgccgcaag gctttcgagg gtcatctctt cgttcatggt
      841 acgggtcctc ccggaaaaaa tatatttttt gcaggcgata ttataggaac gccccccaga
      901 ttatacttca ttgacagcaa gagtcggata tcagcatact ttatggagag cggcatatca
      961 aacaaaggag tgatcacgat ggctgttcct atgaaatttc tgtccgaaga gctcgaaacg
     1021 atgaagcagg gcgggttcta cggcactatc cggactctgg agagccccca gggggcgtgg
     1081 gtccatatcg acggaaagaa gtacctgaat ctctgctcga acaactacct cgggctgtgc
     1141 aacgatcctc gtcttgtgaa caaggtgaag gagtacgcgg acaagtacgg cgtcggcccc
     1201 ggcgcggtgc gcaccatcgc ggggacgatg agcccgcaca tcgagctgga gcaaaagctt
     1261 gccgcgttca agggcgcgga ggcggccatc gtggtgcagt cgggcttctg cgccaacctc
     1321 gcggtgattc cgacgctggc gagttccgcc gacgacgtga tcttcagcga ctcgctgaac
     1381 cacgcctcca tcatcgacgc gtgcaagctg acgaaggcga aggttgtgcg ctacgagcac
     1441 tccgacatgg ccgacctcga gaagaagctg aaggagtacg acggcttcgc agggaagaag
     1501 ctgctgatca cggacggcgt cttctcgatg gacggcgacg tggcgaagct gccggagatc
     1561 gtggagctgt gcgagaagca cggcgtgatc gtcgcggtgg acgacgcgca cggcgagggt
     1621 gtgctggggc gcggcggccg cggcatcgtg gatcacttcg ggctgcacgg ccgcgtggac
     1681 gtggagatcg ggacgatgtc gaaggcgttc ggcgtgatgg gcggcatggc ggcgggaagc
     1741 gcgattcttg tggactacct ccgtcagaag gcacggacga accttttcag cagcgcgctc
     1801 accatccccg acgtggcggc gaacctggcc tcggtggaga tcttacagca gagcgaggat
     1861 ctggtgaaga agctgtggca caacggcgac ttcctgaagc gcgagctgaa agcgctgggc
     1921 ttcgacacgg cgcacagcga gacgccgatc acgccggtga tcatcggcga ggcctcggat
     1981 gcgaaggcgt tcagcgcgaa gctcttcgag gcgggcgtgt tcgctacggc gatcgtcttc
     2041 ccgacggtgc cgcgcggcac ggcgcgcatc cgggcgatgg tctcggcggc gcactccgag
     2101 gacgacctgc gcttcgctgt ggagaagttc gccgcagtgg gcaaggcgat ggggatcata
     2161 aaatgaagaa gattcttgtg acgggggcca acggacagat cggcgtggag ctggtcgtcc
     2221 atctgcggaa gctctacgga gcagaaaacg tgctggcgac cgaccttcgg aagacgcccg
     2281 gcgcggcctc cgagggcggt ccgttcgcgg cgctcgacgt gcgcgacccg aaagcgttct
     2341 ccgagacgct gcgctcgttc ggcgcggaca cggtgctcca ccttgcgggg atcctttcgg
     2401 cgcgggggga ggcggacccg atgttctcgt gggacgtgaa cgtgaacggc acgtgcgcgg
     2461 cgcttgaggc ggcccgcgaa cacggcgcgg ccttcttctt ccccagctcg atcgcggcgt
     2521 tcggcccgtc gacgccctcg aagaacacgc cgcaggacac gctccagcgg ccctcgacgg
     2581 tctacggcgt agcgaaggtg agcatggagc tcctgtgcga ctactaccac aagaagttcg
     2641 gcatggacac gcgcggactg cgcttccccg gcctgatcag ctacgaggcg ctccccggcg
     2701 gcggcacgac ggactacgcc gtgcacatct actacgacgc gatcaagaag gggagctaca
     2761 ccagcttcat cgcgcagggg acgtacatgg acatgatgta catgccggac gcgctgaacg
     2821 ccatcgtccg cctgatggag gccgacccgg cgagacttct gcaccggaac gccttcaaca
     2881 tctcggcgat gagcttcgag ccggagcaga tcgcggcctc catccggaag ttcatgcccg
     2941 gttttacgat ggactacgac gtggacccgc tgcggcaggg gattgccgag tcgtggcccg
     3001 attcgctgga ctgctcggct gcccgcgaag agtggggctt cgccccggag tacgacctcg
     3061 accggatgag cgaggatatg cttgcgcggc tgaaggaaaa gctttgagcg tgcgcccccc
     3121 ggtactccaa ccggggggtt ttttgtattc cggccctgcg gccgggcaca acgagagggg
     3181 gaacgcaaca tgctcgacaa agcgctcccg agcgttctgg gagctatagg gaagacgccg
     3241 ctggtgcgtc tggaccgcat cgcgagggcg tggaatctgg aggggacgct ctacgcgaag
     3301 cttgagtatc tgaatccggg gttcagcaag aaggaccgca tcgccctgca gatgatcgag
     3361 gaggcggagg agcgcggcgt gctccatccg ggacagaccg tggtggagct gacgagcggg
     3421 aatacgggga cggggctggc cattgtctgc ggcgtgaagg ggtacccctt ctgcgcggtg
     3481 atgtcgaag
//
LOCUS       DAOPKU010000317         3488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_30094_length_3488_cov_4.318672, whole genome shotgun sequence.
ACCESSION   DAOPKU010000317 DAOPKU010000000
VERSION     DAOPKU010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30094_length_3488_cov_4.318672"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PK849_09930"
     CDS             complement(<1..1113)
                     /locus_tag="PK849_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HPE66483.1"
                     /translation="MARDIGVTECDVPMEGEAVLRKATKECAYRTEMDPMWTKAIVVL
                     SCVFILYHLVTSRFGMPPMFKHRAIHLGFILCLVWMYYPATSRSIQKRPSFVDMGLMV
                     ASIAVCGYTVLNVDAFALRGGVALPRDYYFGAVAILLVLEACRRVAGKGLLILAVVFL
                     LYAWLGRYIPGVLSHRGYSIQRIIYQMYLTTEGIYGLPIGIAATYLVLFIVFSSFLEK
                     SGLGTLFNDVSLALGGRLVGGPAKVAVIGSALVGMISGSAATNVATSGAFTIPLMKKI
                     GYKPYFAGAIEAAASTGGQIMPPIMGTGAFIMAEFLGVPYISIAAAAVIPAVLYFAAV
                     FFQVDLRARKIGLTGLSKEELPDVKKTLLRYGHMFLP"
     gene            complement(1325..2299)
                     /locus_tag="PK849_09935"
     CDS             complement(1325..2299)
                     /locus_tag="PK849_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HPE66484.1"
                     /translation="MVRIIGRDDIKTAAGRISGRVRRTPVLRGDKLDALFGAEVYFKP
                     ENLQLTGSFKIRGAMNKILSLSDEERAKGIIASSSGNHAQGVAYAAKMLGIKATLVLP
                     ENAPKSKIEGTRALGADVVLHGFDSIQRYKKLYEIKAEKGYTLVHSFNDPELIAGQGT
                     SGLEIIEDIPDVDTVVVPLGGGGLLAGVATAIKETRSSVRIVAVEPESIQRYAESRKA
                     GKPVEVPMGATLADGLMITMTGEHTYPLIDKYVDEIVPASDEFIRKALMEIVFKSKLL
                     VEPSASVGVAAALQGSFKVKPGEKICFFLSGGNIDPEKLASFLPRETA"
     gene            complement(2408..2608)
                     /locus_tag="PK849_09940"
     CDS             complement(2408..2608)
                     /locus_tag="PK849_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE66485.1"
                     /translation="MAVHVNKRLCKGCGLCISVCPKNVYEISNETNQKGFTVVAAVRE
                     GDCIKCKRCEITCPDMALWIEE"
     gene            complement(2615..>3488)
                     /gene="sucD"
                     /locus_tag="PK849_09945"
     CDS             complement(2615..>3488)
                     /gene="sucD"
                     /locus_tag="PK849_09945"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF004230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPE66486.1"
                     /translation="SEMAVLVDGSTRVIVQGITGKSGILQTKSLLDYGTSVVAGVTPG
                     KGGAVVEGVPVFDSVLDAMKSVSPNAAISFVPPMFAKDAAIEAMEVGIKLLVLTMEGI
                     PKHDVLDILSYAQGRGVRVLGPGTAGVISPGKCKLGAHPARMFMEGKVGVVSKSGALS
                     YEVGKTLTDAGIGQSTVVALGGGPIWGTNQREVVELFNADPETEIIVLLGEIGGGTEI
                     AAAEYIAEYVRKPVVSLIVGRAAPEGKSLGHAGAIIRGNKGTAASKMEALEKAGAHLA
                     TSPAQVVEIIRKLG"
BASE COUNT          685 a         1105 c          971 g          727 t
ORIGIN      
        1 cggcaggaac atgtgcccgt accggaggag cgtctttttc acgtcgggca gttcctcctt
       61 ggaaagcccc gtcaaaccga tcttgcgcgc ccggaggtct acctggaaga agactgctgc
      121 aaaatagagg accgccggaa taaccgccgc cgccgctatc gaaatgtaag gaactccgag
      181 aaactccgcc atgatgaacg ctccggtccc catgatcggc ggcatgatct gccctcctgt
      241 tgaggctgcc gcttcaatag ctccggcaaa ataaggctta tacccgattt ttttcatcag
      301 cgggatggtg aaggcgcccg acgttgccac gttggtggcc gcgctgccgc tgatcatacc
      361 gacaagagcg cttccgataa cggcaacctt tgcgggcccg ccgaccagac ggcctccgag
      421 cgcgagggag acgtcgttga agagcgttcc gagtccgctt ttctcaagga aagaggagaa
      481 gacgatgaag aggacaaggt atgtcgcggc aatgccgatg gggagtccat agataccttc
      541 cgtggtgagg tacatctggt agatgatgcg ttggatcgag tagccccggt gggagagaac
      601 gccgggaatg taccggccga gccaggcgta cagcaggaag acaacggcca gaatgaggag
      661 tcctttccct gcgactctgc ggcaggcctc aaggacgagc agtatcgcca ccgcgccgaa
      721 gtagtagtcc ctgggaagcg caacgccgcc tctgagcgcg aaggcgtcaa cgttgagcac
      781 cgtgtagccg cagacggcga tggaggcgac catcagcccc atgtcgacga aactcggtct
      841 cttctggatg gagcggctcg tcgcggggta gtacatccac accagacaga gaatgaatcc
      901 gagatgaatc gccctgtgct tgaacatggg aggcatcccg aacctggagg tgacaagatg
      961 atagagtata aacacgcatg acaggacaac gatcgccttc gtccacatcg ggtccatctc
     1021 cgtcctgtac gcgcattcct tcgtggcctt tctcagaacg gcttcccctt ccatggggac
     1081 atcacactcg gtgacaccga tatctcgcgc cattcatttc cctccactcc tgaaatattt
     1141 actcgttcat gctttatgca ttatatattg tatatgaagc tcaaaggcat tgcaaagtgc
     1201 atactgtatt cttgagtggt acagtgaact ctctttacga tacgtctggc aaacactggc
     1261 ggaaaagcgg cgggaagggg tgcgttttca tggccggctc gttccgcttc gccgcccctg
     1321 aatattacgc cgtctctcgt ggtagaaagg atgcaagctt ctccgggtcg atgttgccgc
     1381 cggagagaaa gaagcatatc ttctcgccgg gtttgacctt gaaggaaccc tgaagcgccg
     1441 cagccacgcc gaccgaagcg gagggctcga ccagcagttt gcttttgaag acgatctcca
     1501 tgagcgcctt ccggataaac tcgtccgacg cgggaacgat ctcatccacg tacttgtcga
     1561 tcagcggata cgtgtgctct ccggtcatcg tgatcatcag gccgtccgcg agcgtcgccc
     1621 ccatggggac ttctacgggc ttccccgcct tgcggctctc ggcgtaccgc tggatagatt
     1681 ccggttcaac agcgacgatc cgtacggagg aacgggtctc cttgatggcc gtagctacgc
     1741 ccgcgagcag tcctcccccg ccgagcggta cgaccacggt atcgacatcc ggaatatcct
     1801 cgatgatctc cagcccggac gttccctgcc cggcaatcag ctcgggatcg ttgaaggagt
     1861 ggaccagcgt gtaccccttt tcggccttga tctcatagag cttcttgtat cgctggatgg
     1921 agtcgaaacc gtggagcacg acgtccgcac caagcgctct cgttccttct atcttcgatt
     1981 tgggagcgtt ctccggaagc acgagcgtcg ccttgatgcc gagcatcttc gccgcatagg
     2041 cgactccctg cgcgtggttc cccgaggacg atgcgataat accttttgcc cgttcctcgt
     2101 cggagaggga aaggattttg ttcatcgcgc ccctgatctt gaaggaccct gtaagctgaa
     2161 ggttctccgg tttgaagtag acctccgcgc cgaagagcgc gtcgagttta tctccccgca
     2221 gaaccggcgt ccgccgaact ctcccggaga tcctcccggc agctgtttta atgtcgtcac
     2281 gtccgatgat tcgtaccatg ttccctttcc gcctccttgc gcccccgagg ggcgaaatgc
     2341 attccttcaa aagcccggag acggccggaa gaagcttccc gcgccgcccc gttctcgtac
     2401 cgctattcta ttcctctatc cacagcgcca tgtcggggca ggtgatctcg caccgtttac
     2461 acttgatgca gtctccctcg cgaaccgcag ccacgacggt gaaccccttc tggttcgtct
     2521 cgttggatat ctcgtagacg ttcttcgggc agaccgagat gcacagtccg caccctttgc
     2581 agagacgctt gttcacatga acggccatct tcactcaccc cagcttccgg ataatctcca
     2641 ctacctgggc gggggacgtc gccagatgcg cccccgcttt ttccaaggct tccattttcg
     2701 acgcggcggt tcccttgttc ccccggatga tcgccccggc gtgtcccaag gactttcctt
     2761 cgggagcggc cctgccgacg atcaacgaga cgacgggttt ccgcacgtac tcggcgatgt
     2821 actccgccgc ggcgatctcc gtgcctccgc cgatctctcc gagcaggacg atgatctccg
     2881 tctcggggtc cgcgttgaac agctcgacca cctcgcgctg gttcgtcccc caaatcgggc
     2941 cgccgcccag cgcgacgacg gtggactggc ctatccctgc gtccgtgagc gtcttgccga
     3001 cttcgtagga gagagctccg ctcttggaga ccacgcccac cttcccctcc atgaacatac
     3061 gcgcagggtg cgcgcccagc ttgcacttgc ccggcgagat gacgcccgcc gtgccggggc
     3121 cgagcacgcg cacgcccctg ccttgagcat aggagaggat atcgagcaca tcgtgcttgg
     3181 gtattccctc catcgtgagc acgagcagct tgatgcctac ctccatcgcc tcgatcgccg
     3241 cgtccttcgc gaacatcggc ggcacgaagc tgatggcggc gttcggagaa acgctcttca
     3301 tcgcgtcgag gacggagtcg aaaacaggta cgccctcgac gactgcgccc cccttgcccg
     3361 gcgtcacccc ggcgactacc gatgttccgt agtcaagaag ggacttcgtc tgcaggatcc
     3421 ccgacttccc tgtaatcccc tgaacgatga cccgcgtgct tccatcaacc agaacggcca
     3481 tttcactc
//
LOCUS       DAOPKU010000318         3478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_30229_length_3478_cov_5.467134, whole genome shotgun sequence.
ACCESSION   DAOPKU010000318 DAOPKU010000000
VERSION     DAOPKU010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30229_length_3478_cov_5.467134"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK849_09950"
     CDS             complement(<1..354)
                     /locus_tag="PK849_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HPE66487.1"
                     /translation="MSVQSSWDRMPPVSLEAERSALGACLMDREALNIVMEILQPEDF
                     YDTTHRAAFEVIFGMAQRDKPVDPLTFLEEIARQGKGDLFGGQAFVGSLIDSVPTTAN
                     VEYHAGIVRDKAIHRR"
     gene            complement(355..801)
                     /gene="rplI"
                     /locus_tag="PK849_09955"
     CDS             complement(355..801)
                     /gene="rplI"
                     /locus_tag="PK849_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPE66488.1"
                     /translation="MKVILKQDVNKLGASGDLVEISDGYARNFLFPRNLADEATPERM
                     KEWKDKEAARKKKEVRLEKEARELQKKLSGRAVKVKASIGEKGRLFGSITAANVAESI
                     VSQLAVEVEKKNLRMPESVKQIGSYSFTVRLHPGIEAEMTLIVEGE"
     gene            complement(798..1769)
                     /locus_tag="PK849_09960"
     CDS             complement(798..1769)
                     /locus_tag="PK849_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HPE66489.1"
                     /translation="MTSTRSLVESALLAGFAVVLFLAAHFLPLIGVAFSLLAPAPLVI
                     LGLRHDLKKATLGLGVSTLLVASLLGPLSALFFVLGFGVLGVGLGFLAKRCEKGVEVL
                     LYGILLSLGSKLLLMVIAGKVTGINPFQLDGAEMQSMIDKIFLFYESTGMSKESIAAV
                     REQFAESLRLLPVIFPTILTMAAALDCYLSYTISSFVLKRVGGTPLPPLPPFSMWRFP
                     KSVFGALVASILLSLFGSQSGEWNFALRAGTNLRLLVNFLFLFQGLSLIWYFFSEKAR
                     VPFGRVLRTVAIIVVVLVPLFSTIAVMAGIGDMWLDFRRRFGRSDAS"
     gene            complement(1854..2150)
                     /gene="rpsR"
                     /locus_tag="PK849_09965"
     CDS             complement(1854..2150)
                     /gene="rpsR"
                     /locus_tag="PK849_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPE66490.1"
                     /translation="MNEGRGEGRGEGRPEGRGGAGPRGPRKGGKRRPKVCFFCVDKVE
                     HVDYKDVEKLRKYVSERGKIMPRRVTGNCAKHQRQLTEAIKRARYMALLPFSLE"
     gene            complement(2184..2672)
                     /gene="ssb"
                     /locus_tag="PK849_09970"
     CDS             complement(2184..2672)
                     /gene="ssb"
                     /locus_tag="PK849_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPE66491.1"
                     /translation="MAKGFNKVVLMGNLARDPEVRYTAGKQAVANFPLAVNRTWKDKN
                     GELQESVDFIPVVVWGSTAENCERYLKKGSPAFVEGRLQVRSYEAKDGSGKRYVTEVV
                     AAEVIFLGSKKQDAPSDSFEDEGFGDSIRKDRRFSGGSSDDDFPMDISSVQGEEDADI
                     PF"
     gene            complement(2685..2969)
                     /gene="rpsF"
                     /locus_tag="PK849_09975"
     CDS             complement(2685..2969)
                     /gene="rpsF"
                     /locus_tag="PK849_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPE66492.1"
                     /translation="MRPYEMMVLVSAELEDPKEEIAKVEEVVRSLEGEIVKTDFWGKR
                     RLAYPIDKKTEGFYALCSFKLKPDQLGELHRVLGLRPNIYRQMTIRLDEK"
     gene            complement(3049..3351)
                     /locus_tag="PK849_09980"
     CDS             complement(3049..3351)
                     /locus_tag="PK849_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HPE66493.1"
                     /translation="MLRLRWILLAALLSLFAAIMGTSYFLEIQKINRLTALVDERMAI
                     LVSMSRTVQELREKIAFYGTTEGLAHLAREQYNLSFPGELVFKIEKKKNSLPEGRQ"
     gene            complement(3356..3472)
                     /locus_tag="PK849_09985"
     CDS             complement(3356..3472)
                     /locus_tag="PK849_09985"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HPE66494.1"
                     /translation="MLGEEVRVMKARLLLVQAAGTVLSTALELLGVSAPARM"
BASE COUNT          847 a         1036 c          861 g          734 t
ORIGIN      
        1 cctgcggtgg atcgccttat ccctgacgat accggcgtga tactccacgt ttgcagtggt
       61 gggaacgctg tcaataagcg aaccgacaaa cgcctgccct ccgaagaggt cgcccttccc
      121 ctggcgggcg atctcctcaa ggaaggtgag ggggtcaacg ggtttgtcgc gctgcgccat
      181 gccgaagata acctcaaaag cagctcgatg ggtcgtgtcg tagaaatcct ccggctggag
      241 aatctccata acgatattga gagcctccct gtccatgagg caggcgccca gcgcggatcg
      301 ctccgcctca agagagaccg gcggcatccg gtcccaggaa gactggacgg acatctactc
      361 gccttcgacg atgagggtca tctccgcctc tattccggga tggaggcgaa cggtaaaaga
      421 atagcttcct atctgcttca cagactccgg catacggaga ttttttttct ccacctcgac
      481 cgcgagctgc gagacaatgc tctccgcaac gttggcggcc gtgatgctgc cgaagaggcg
      541 gcctttttcg ccaatactcg cctttacctt cacagcgcgc cctgaaagct tcttctggag
      601 ttcgcgcgcc tctttttcca gccgaacctc ttttttcttt ctggcggctt ccttatcttt
      661 ccactccttc attcgctccg gagtggcctc gtcggcaaga ttgcgcggaa acaggaaatt
      721 acgtgcatat ccatccgaaa tctcaacaag atcgccggat gcgcccagtt tatttacatc
      781 ttgctttaaa atgactttca tgaagcatca ctccttccga aacgtctccg gaaatcgagc
      841 cacatatcgc ctattccggc catgaccgct atggtggaaa aaagaggaac cagcactact
      901 actattatag caacggttcg aagaacccgc ccgaaaggga cgcgagcttt ctccgagaag
      961 aaataccaaa taagagagag cccctgaaag agaaagagaa agttcaccaa aagacgtaaa
     1021 ttggtccctg ccctgagagc aaagttccac tcgccgcttt gcgagccgaa gagagagagg
     1081 agaatggacg cgacaagggc gccgaaaacg ctcttcggga aacgccacat tgagaaaggg
     1141 ggaaggggag ggagaggcgt tcctccaacg cgcttgagca cgaaagaact gatggtgtag
     1201 ctcaagtagc agtcaagcgc ggcggccatc gtcagaatgg tagggaagat aacgggcaga
     1261 agacggagag actcggcgaa ctgctctcga accgctgcga ttgattcctt gctcatgcca
     1321 gtcgactcgt aaaacaggaa aatcttgtcg atcatcgact gcatctcagc gccgtcgagc
     1381 tgaaacggat tgatccccgt cacttttccc gcgatgacca tcaggagaag cttacttccc
     1441 agggagagaa gaattccgta caagagaacc tccacaccct tttcgcagcg tttcgccaga
     1501 aaaccgagtc ccactccgag cacgccgaag ccgagcacga aaaagagcgc cgagagcggc
     1561 ccgagaagcg acgcgacaag gagcgtcgat acgccgagcc ccagcgtggc ttttttcaga
     1621 tcatggcgca gccccagtat tacaagaggg gcgggggcaa gaagagagaa cgctacaccg
     1681 ataagcggaa gaaaatgcgc ggcaagaaag agcaccaccg caaaaccagc gagaagagcc
     1741 gattcaacca ggcttcgtgt cgaagtcaca ccgcaacctc ccttcgaaaa actaaaagga
     1801 cggggggaga agccaggctc ctccccccga aaagaacgtt tcagataggc gccctactcc
     1861 agagagaacg gaaggagagc catataccgg gcccttttga tcgcctcggt gagctgacgc
     1921 tgatgcttcg cgcaatttcc cgtcacacgg cgcggcatga tctttccgcg ctcgctcacg
     1981 tactttcgaa gcttttcgac atctttgtaa tcaacatgct caactttatc tacgcagaaa
     2041 aaacatacct taggccggcg tttcccgcct tttcgaggcc ctctgggacc ggctccgcct
     2101 ctgccttcgg gcctgccttc gcctctgcct tcgcctctac cttcattcat tgccattgtg
     2161 cactgtcctc cttcaaaaaa cacctaaaag gggatatccg catcctcttc gccttgcacc
     2221 gaggaaatgt ccatcgggaa gtcgtcgtcc gaactcccgc cgctgaagcg gcgatccttc
     2281 cggatgctgt cgccgaaccc ctcgtcctcg aacgaatcgg aaggagcgtc ctgcttcttc
     2341 gagccgagga aaataacttc ggcggcaacc acttcggtga cgtaccgttt ccctgaaccg
     2401 tccttggctt cgtaactccg aacctgaagt ctgccctcga cgaaagcggg actccctttc
     2461 ttcaagtaac gttcacagtt ttcagcagtg gacccccaga caacaacagg aataaagtca
     2521 acgctctcct gcaattcgcc gtttttatcc ttccacgttc tgtttacagc aagcggaaag
     2581 ttcgcaacgg cctgttttcc ggcggtatac cgcacttcag gatctcgagc cagattcccc
     2641 atgagtacga ctttattgaa ccctttcgcc acgaccccgc ctccttactt ttcgtcaaga
     2701 cggattgtca tctgacgata aatgttcggg cgcaaaccaa gaacacggtg aagctctcca
     2761 agctgatcgg gcttcagctt gaagctgcac aatgcgtaaa aaccttccgt tttcttatcg
     2821 atggggtagg caagacgcct ttttccccag aaatcagtct tgacgatctc cccctcaaga
     2881 ctgcgcacga cctcttcaac cttcgcgatc tcctctttgg gatcctccag ttcggcgctc
     2941 acaagcacca tcatttcata tggccgcaca ctcattcacc tcctccctct ggactctggc
     3001 cccttttcaa agaaaggggc agggaaaaac ttgccatggg attatacatt attgtctccc
     3061 ttcaggcaag gaattcttct ttttttcgat tttaaaaacc agctctcccg ggaaggagag
     3121 gttgtactgt tcgcgggcaa ggtgcgccag cccctccgta gtgccgtaaa acgctatctt
     3181 ctcccgaagc tcctgcacag tgcggctcat cgagaccagg atcgccatcc gttcatcgac
     3241 aagagccgta agacggttga tcttttggat ctccagaaaa taggacgtcc ccattatggc
     3301 cgcaaaaagg ctcagaagcg ccgcaagcag aatccaacgg agacgaagca cattcctaca
     3361 tcctcgcggg agcggaaact cctagcagct caagcgctgt cgaaagtaca gtgccggcag
     3421 cctggacaag gagcagacgc gctttcatca ctcttacctc ctcgccgaga atgcggca
//
LOCUS       DAOPKU010000319         3410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_31057_length_3410_cov_4.449478, whole genome shotgun sequence.
ACCESSION   DAOPKU010000319 DAOPKU010000000
VERSION     DAOPKU010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31057_length_3410_cov_4.449478"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..2036
                     /locus_tag="PK849_09990"
     CDS             81..2036
                     /locus_tag="PK849_09990"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPE66495.1"
                     /translation="MRKCLWILVFACLVWGFAAAPLFAEERILRFDVEATVEEGASLI
                     VTERIAFVAEGNDIKRGLYRTFPTDFTDTEGVRRRAGFDLLSAELDGAPTPSLVERVG
                     ENLDIRLGDLEKLLAHGEHQFTITYRTSGQLGFFGEHDELYWNVTGNEWAFPIEKVSF
                     RLRLPGRDFGADFSSIEFYTGKKGERGQDAFVTKEGTVESTRLLSQGEGLTVVYTWPK
                     GIVAPPAEPAPVLEKWTPSPYRVAHLAMPVVLALVMTLLWILWGKDPPAKAVFPRFAP
                     PQGIEAGFSRYVRSMRMDDQAFAAMVLGMAVKGPLTIEERSLVAEAAKQTGKDASQAS
                     MGMKLLSKLVGKSYVLRLNREKLSNTNLTIDERVLVDELFGSTRSDIHLSSADRPVIQ
                     DAFGQLGKRFKERAKPLLKTNTGKWLAGVALFEIYAVVMLVLMILSGEGRFEPVLALM
                     AGPFLLLPFAIPVPSGKGSMMSKFFLRVFFPGIFLFVTAGAVLAGSTSGIEVDLLSVP
                     GPFLCVLILLLFKPLMRVRSEEGARLNEELEGLRMFMTAAEKHRLELGNPPEETPKLF
                     EELLPYAFALDVAETWANRFENILAAAHYTPSWYRGDMHTFTQTTGVAAFASGFAGAV
                     ASGTRSSGRGGSGSSGGGGGGGGGRGW"
     gene            2070..2795
                     /locus_tag="PK849_09995"
     CDS             2070..2795
                     /locus_tag="PK849_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPE66496.1"
                     /translation="MRVVKICAVLASILLLSVPLAANPTVESFEWPKSGMLVGERVNL
                     RSGPATSSKVIGRFVDEYEGGELLVTGRSEGREEFPWYRVVSHKFGEGWLYGKFIAVE
                     EPEDPVRRYALKIREDFGLSPAQAVKMYGEPMKRSREKLHIPDFNVTAEIVTLEFHGH
                     TAIYWDGVLQSVEIPAGFMGFADILLGMDAEEAASRLGTPILRQEDTLIFGFERDEIA
                     LTEGKTGGRTVVAGLAYRRVVYD"
     gene            2814..>3410
                     /locus_tag="PK849_10000"
     CDS             2814..>3410
                     /locus_tag="PK849_10000"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HPE66497.1"
                     /translation="MKFAVVPVLCLLLLCGAGWGGEHPLDVEIQECMDKDPSTHGTIK
                     CAREGAKKWDTELNRAYKELMGLLPKQGQDALRTAQRAWIPWRDREFGLVGSVYGAIY
                     NSLDGGTMWLVVNAIAEMEVVRGRTLELIAWSEEMKKGKPVFEGAYPEKQTDDQLAAA
                     MRVKNASLRLGKRIGKEGENTAAANLAAWEEFRNRNVLF"
BASE COUNT          731 a          856 c         1076 g          747 t
ORIGIN      
        1 tagttcacga atttcaagaa gctccgggga tatgacctcg gagcttcttt ttcctctccc
       61 atgcaaggag gtggaacgcc atgagaaaat gtttatggat acttgtattc gcgtgtctgg
      121 tatggggttt tgcagcagcg ccgctttttg cagaggagcg aatactccgt ttcgacgtcg
      181 aagcaacggt ggaagaggga gcctccctta tcgtaacaga gcgcatcgcg tttgtggcgg
      241 agggaaacga cataaagcgt ggactctaca gaactttccc gactgatttt accgatacgg
      301 aaggcgttcg cagaagggct ggttttgatc ttctttccgc ggagcttgac ggggcgccga
      361 ccccatcgtt ggtagaacgt gtcggagaaa acctcgatat tcgcctcggc gacctcgaaa
      421 aactccttgc tcacggcgag catcagttta caataacata caggacatcg ggacagctcg
      481 gttttttcgg ggaacacgac gaactgtact ggaacgtcac aggaaatgaa tgggcgttcc
      541 cgatagaaaa ggtatcattt cgtctacgac tccccggaag ggatttcggc gctgattttt
      601 cgagcataga gttctatacc ggaaaaaagg gagagcgggg gcaggatgca ttcgtaacga
      661 aagaagggac ggtggagtct actcggttgc tttcgcaagg cgagggcttg acagtagtct
      721 acacatggcc gaagggcata gtcgcccctc cggctgaacc cgcgcccgtt ctggaaaaat
      781 ggacgccttc cccgtacaga gtagcgcacc ttgcaatgcc cgttgttctt gccctggtga
      841 tgacgctgct ctggatactg tggggcaaag atccccccgc aaaagcagta ttcccccggt
      901 ttgcccctcc tcagggaatc gaggctggct tctcccgcta cgtacgatcg atgcggatgg
      961 acgaccaggc gttcgccgca atggttctgg ggatggccgt caagggaccc ttgacgatcg
     1021 aggagcgttc tcttgttgcg gaggcggcga agcagacggg aaaagatgct tcgcaggcca
     1081 gtatgggtat gaagctgctt tcaaagcttg tcggaaaatc ctatgttctt cgcctgaaca
     1141 gggagaagct ctcgaatacc aacctcacca tcgatgaacg ggttcttgtc gacgagcttt
     1201 tcggctccac ccgaagcgac atccatctct caagcgcgga ccgtcctgta attcaagatg
     1261 ctttcggaca gcttgggaaa cgcttcaagg agagggcaaa accgcttctg aagaccaaca
     1321 ccggaaagtg gctcgctgga gtcgctcttt tcgagatcta cgccgtcgtc atgcttgttc
     1381 tcatgatcct ctccggcgag gggcgttttg aacctgtgct tgctctgatg gccggaccgt
     1441 ttctcctgct tcctttcgcc attcccgttc cgtcgggaaa gggaagtatg atgtcgaaat
     1501 ttttcctcag agtttttttc cccggcatct tcctcttcgt tacggcaggg gctgtgctgg
     1561 cgggttcgac cagcgggatc gaggtcgacc tcctctcggt tcccggtccg ttcctctgtg
     1621 tgctgattct gcttctcttc aaacccctga tgcgcgtccg ctccgaggaa ggcgccagac
     1681 tgaacgagga gcttgaaggc cttcgcatgt tcatgacggc agcagagaaa caccggcttg
     1741 aattgggaaa ccctcctgaa gagacgccga agctctttga ggagcttctt ccctacgcct
     1801 ttgccctcga cgtcgccgag acgtgggcga accgttttga gaacattctt gccgccgcgc
     1861 actatacgcc gtcgtggtac agaggggata tgcatacctt cacacagacg accggcgtcg
     1921 ccgcgttcgc ttcaggtttt gcaggcgcag tcgcgtccgg aacgcgctcc tcggggagag
     1981 gcggaagcgg ctcctcggga ggagggggcg gcggaggcgg aggacggggc tggtagtatt
     2041 ctttctttat cgaaaggagg gaagggataa tgagagtagt aaaaatctgc gcggttttgg
     2101 cgagtatcct ccttctctct gttccgttgg ccgcaaatcc gacggtggag agcttcgaat
     2161 ggccaaagtc gggcatgctg gttggggaga gggtgaatct tcgttcaggg ccggcaactt
     2221 catcgaaggt catcggccgt ttcgtggacg aatacgaagg gggcgaactc ttggtgacgg
     2281 ggagatccga aggtcgcgaa gagttcccgt ggtatcgtgt agtctctcac aaattcggag
     2341 aagggtggtt gtatggcaaa ttcattgccg tcgaggaacc cgaggatccg gtgcgccgtt
     2401 acgcgctgaa aatccgcgag gacttcgggc tctctcctgc tcaagccgtg aagatgtacg
     2461 gcgaaccgat gaagcgttct cgtgagaaac tgcatatccc cgacttcaat gtgacggcgg
     2521 agatcgtgac gcttgaattc cacggccata ccgcgatcta ttgggacggt gtgctccagt
     2581 cggtggagat accggccgga ttcatggggt tcgcggatat tcttcttgga atggacgcgg
     2641 aagaagcggc ttcacggctc ggtacgccta ttctgcggca ggaagatacg ctgattttcg
     2701 gcttcgagcg cgacgagata gcgctcacgg agggaaagac gggcggcagg acggtcgtcg
     2761 ccgggttggc ctaccgccgg gttgtctatg attgaagatg gaggtggggt ttcatgaagt
     2821 tcgcggttgt gccggtgctc tgcctgctcc tgctgtgcgg cgccgggtgg gggggcgaac
     2881 accctctgga tgttgagatc caggagtgta tggacaagga tccgagcacc cacggaacga
     2941 tcaagtgcgc ccgggaaggg gcgaagaagt gggatacgga gctcaacaga gcatataaag
     3001 agctgatggg gctgcttcca aaacagggac aggatgccct gcgaacggcc cagcgggcct
     3061 ggatcccctg gagagacagg gagttcggac tggtgggttc ggtgtacgga gcgatctaca
     3121 acagtctcga cggtggcacg atgtggcttg tcgtcaacgc tatcgcggag atggaggtcg
     3181 tcaggggaag aacgctcgaa ctcatcgcat ggtcggagga aatgaaaaaa gggaagccgg
     3241 ttttcgaggg cgcgtatccg gaaaaacaga cggatgatca gcttgccgcc gcgatgaggg
     3301 tgaagaacgc aagtctccgc ctgggaaaac gaatcgggaa agagggagag aataccgctg
     3361 cggcaaacct tgcggcgtgg gaggagttca ggaacaggaa tgtgctcttc
//
LOCUS       DAOPKU010000320         3410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_31058_length_3410_cov_4.163338, whole genome shotgun sequence.
ACCESSION   DAOPKU010000320 DAOPKU010000000
VERSION     DAOPKU010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31058_length_3410_cov_4.163338"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PK849_10005"
     CDS             complement(<1..615)
                     /locus_tag="PK849_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPE66498.1"
                     /translation="MIELLQFEFMRNALWAALLCSGLCGILGTVVVVKRYVILAGGVA
                     HAAYGGVGIALYLGISPQLGAVGFSLALSLLMAHVMLKREARADSIIGVLWAAGMAAG
                     VIFADLTPGYGADLMSYLFGSILTVAKADLFMMGGLLAVTLVLGFVWRRQIAAFSFDE
                     DFARTRGVPVALLHFGAVVLLSMAVVLIIRIVGLILVIALLTIPP"
     gene            complement(608..1399)
                     /locus_tag="PK849_10010"
     CDS             complement(608..1399)
                     /locus_tag="PK849_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66499.1"
                     /translation="MHTEPALSFTNVAFSYGGDPVLQDVSFSVPAGEFLVIMGPNGGG
                     KTTLLKLALGLLSPGSGTVLIHGRAPGGDRSAAGYVPQDAGRNRDFPITALEAVLHGR
                     LGAGSHGLFYSVADKTAARDALERMGVESLASRPMGELSQGQRQRVLIARALASEPKV
                     LLLDEPLASIDPETRDMLLTELASLRSRMTVIMVSHDISAIPGRATAVACVNRNVFYH
                     DSGEILPGMFEKVWGTCPVELVAHGIPHRVLAVHTHDHSEDCAHD"
     gene            complement(1405..2928)
                     /gene="pap"
                     /locus_tag="PK849_10015"
     CDS             complement(1405..2928)
                     /gene="pap"
                     /locus_tag="PK849_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HPE66500.1"
                     /translation="MLEAVDMSVKMKKKEYRAAFDELAPKLGELQRIAKEKGVPVILL
                     VEGWEGSNKGSLVNQLLQALDPRGYLFIAPPGRGEERSPFLIPYWEITPPAGKMTILS
                     GSWYREVLRRRLEKGEGAKDYSGVFRDICAFERHLADSGTVVLKFFCHLEKKEQKKRL
                     EARAADPDLSWSVGEADWLENREYEAFLFCAEEMLSFTGTEYAPWTVVAAGDFEFAAW
                     KILSTVERVLRERLSGEEWPATGGVLGGVEYEKSAALSTTEPDGDLSAKEYEKRLAAL
                     QSELKSLEIRVFRESLPVILLFEGVDAAGKGGAIRRITGSLYPMNYTVYPVGAPNDLE
                     RSHHYMWRFWKDFPRYGHIAIFDRSWYGRVLVERVEGFCSPAEWHRAYREINETERHL
                     TDFGTLLLKFWLQIDQEEQLRRFESRKDDPFRAWKLTDEDWRNREKWEDYQKAAGEML
                     VRTGTPYAPWTVVATNSKKMARIKILTTVTEGIRKALDGRAARQKPGEPRNTKRKKA"
     gene            complement(2976..>3410)
                     /locus_tag="PK849_10020"
     CDS             complement(2976..>3410)
                     /locus_tag="PK849_10020"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPE66501.1"
                     /translation="KEAFAVRDPEKTVGNLIDKSPVSLIGSVDEEGFPNTKAMLPPRK
                     REGIKRLYFTTNTSSMRVQQYGLNPKACVYFFDKRFFRGVMLKGTMEVLHDPETKETI
                     WRDGDTMYYPGGVTDPDYCVLRFTAESFRFYSDFKSESCAIE"
BASE COUNT          607 a         1182 c         1011 g          610 t
ORIGIN      
        1 gggggggatg gtgagcagcg cgatgaccag aatcagcccg acgatgcgga tgatgagcac
       61 cacggccatc gagagcagca ccaccgcgcc gaagtggagg agcgcgacgg gaacgccccg
      121 cgtacgggcg aagtcctcgt cgaacgagaa ggcggcgatc tgcctgcgcc agacgaatcc
      181 gagaacgagc gtcacagcga gcagtccgcc catcatgaag aggtcggcct tggcgacggt
      241 gaggatgctg ccgaagagat agctcatcag atcggctccg taccccggcg taaggtcggc
      301 gaagatgacg cccgccgcca ttcctgcggc ccagagcaca ccgatgatgg agtccgcccg
      361 cgcctcccgc ttcagcatca cgtgcgccat cagcagcgaa agagccagcg agaagcccac
      421 ggccccgagc tgcggtgaaa tcccgaggta gagcgcgatg cccacgccgc cgtacgccgc
      481 atgcgcgacg ccccccgcga ggatgacgta gcgcttcacc acgaccacgg tcccgaggat
      541 gccgcagagc ccgctgcaca gcagggccgc ccagagggcg ttccgcatga actcgaactg
      601 gagcagctca atcatgagcg cagtcctcgg aatggtcgtg cgtgtgaacg gcgagcaccc
      661 tgtgcgggat gccgtgcgcc accagctcca cgggacacgt cccccagacc ttctcgaaca
      721 tgcccgggag aatctccccc gagtcgtggt agaagacgtt tcggttgacg cacgccacgg
      781 ctgtggcgcg ccccggaatg gcggagatgt cgtggctcac catgatcacc gtcatcctcg
      841 aacggagcga ggccagctcc gtcagcagca tgtcgcgcgt ctccgggtcg atgctcgcca
      901 acggttcgtc gagcagcagc accttcggct ccgaagccag cgcgcgcgcg atcagcaccc
      961 gctgccgctg cccctgcgaa agttcgccca tcggccggga ggccagagac tccaccccca
     1021 ttcgttcgag cgcgtcgcga gccgccgttt tatccgctac gctatagaag agcccgtgcg
     1081 accccgcgcc gagacgcccg tggagcaccg cttccagcgc cgtgatgggg aagtcgcggt
     1141 tgcgccccgc gtcctgcggc acgtaccctg cagcgctccg gtcccctccg ggcgccctcc
     1201 cgtggatcag caccgtcccc gaaccggggg agagcagtcc gagcgcgagc ttcagcaggg
     1261 tggtctttcc cccgccgttc ggccccatga tcaccagaaa ttcgcctgcg ggaacggaaa
     1321 atgatacgtc ctgcagaaca gggtcgcctc cgtaggaaaa agcgacgttc gtaaaggaga
     1381 gagccggctc cgtatgcact cgttttatgc cttctttcgt ttcgtattcc gcggttcgcc
     1441 gggtttctgc ctcgcggcgc gcccatccag cgctttgcgg atgccttccg tcaccgtggt
     1501 cagtatcttg atgcgcgcca tctttttgga gttcgtcgcc acgactgtcc acggcgcgta
     1561 gggcgtgccg gtgcgcacca gcatctcccc agctgccttc tggtagtcct cccacttctc
     1621 gcggttgcgc cagtcctcgt cggtcagctt ccacgcgcgg aaggggtcgt ccttccggct
     1681 ctcgaagcgg cgaagctgct cctcctggtc gatctggagc cagaacttga gcagcagcgt
     1741 gccgaaatcg gtcaggtggc gctccgtctc gttgatctca cggtaggccc gatgccactc
     1801 cgcgggggaa cagaacccct cgacgcgctc gacgaggacg cgcccgtacc agcttctgtc
     1861 gaagatggcg atatgtccgt acctggggaa gtccttccag aaacgccaca tgtagtggtg
     1921 gctgcgttca aggtcgttcg gcgcgccgac cgggtacacc gtgtagttca tcggatagag
     1981 cgaccccgtg atcctgcgga tcgccccgcc ctttccggcg gcgtccaccc cctcgaagag
     2041 gaggatcacc ggcagcgact cgcggaagac gcggatctcc agcgacttca actcgctctg
     2101 taaggcggcg agccgcttct cgtactcctt agcgctcagg tcgccgtccg gctccgtcgt
     2161 cgacagcgcc gcgctcttct cgtactccac gccgccgagg acgccgccgg tcgccggcca
     2221 ctcctcgccg gagagcctct cccgcagcac gcgctcgacc gtggagagga tcttccacgc
     2281 ggcgaactcg aaatcccccg ccgcgaccac cgtccacggc gcgtattccg tcccggtgaa
     2341 agagagcatc tcctccgcgc agaagagaaa ggcttcgtac tccctgtttt ccagccagtc
     2401 ggcctcgccc accgaccagg agagatcggg gtcggcggcg cgggcttcaa ggcgcttctt
     2461 ctgctccttc ttctccaggt ggcagaagaa cttgagcacc accgtgccgc tgtcggccag
     2521 atgccgctcg aaggcgcaga tgtcgcggaa gacgccggag tagtccttcg ccccctcgcc
     2581 cttctccagc ctgcggcgga gcacctcgcg gtaccagctc cccgagagga tcgtcatctt
     2641 tcccgcgggc ggcgtgatct cccagtacgg aatcaggaag ggagagcgct cctcgccgcg
     2701 ccccggaggc gcgatgaaga ggtaaccgcg ggggtcgagc gcctggagga gctggttgac
     2761 gaggctcccc ttgttcgagc cctcccaccc ttcaacgagc aggatgacgg gaaccccctt
     2821 ctccttggcg atccgctgca gctccccgag cttcggcgcc agctcgtcga aggccgccct
     2881 gtactccttc ttcttcatct tcaccgacat atccaccgct tcaagcattc ttccgcctcc
     2941 cttccgtaga tttactcaac gccgtcgtct gaaattcact cgatcgcaca actctcggac
     3001 ttgaaatcgc tgtaaaaccg gaaactctcg gcggtgaatc gcaagacgca gtaatcgggg
     3061 tccgtcaccc ctcccggata gtacatcgta tcgccgtccc gccagatagt ctctttcgtc
     3121 tccggatcgt gcagcacctc catcgtgccc ttcagcatga cgcctctgaa aaatcgcttg
     3181 tcgaagaagt agacgcacgc tttcggattc agcccgtact gttgcacgcg catggaggac
     3241 gtattcgtcg tgaaatagag gcgcttaatc ccctctctct ttcggggagg gagcatcgcc
     3301 ttggtatttg gaaacccctc ttcgtcgaca gagccgatca gcgacaccgg cgacttgtcg
     3361 atcaagttcc ccaccgtctt ttcaggatct cgcactgcaa aggcctcctt
//
LOCUS       DAOPKU010000321         3408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_31084_length_3408_cov_4.717268, whole genome shotgun sequence.
ACCESSION   DAOPKU010000321 DAOPKU010000000
VERSION     DAOPKU010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31084_length_3408_cov_4.717268"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PK849_10025"
     CDS             complement(<1..1020)
                     /locus_tag="PK849_10025"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HPE66502.1"
                     /translation="MDQGFRMEKDSLGEVQVPASKLWGAQTQRSLQNFRIGAEKMPEE
                     IIAAFALLKKAAARTNARLGLLDEVRMRAICGAADEILERKHDDQFPLVVWQTGSGTQ
                     TNMNVNEVIARRATELCGGETVIHPNDHVNRSQSSNDTFPTAMHVAAVLALEDSLLPT
                     LESLRSTLGKKATQFAGVVKIGRTHLQDATPLTLGQEISGWARMLERDEEMIRSGLEF
                     LRELALGGTAVGTGLNAHPDFAASVAEELSMLSGKEFRTAPNKFHSLTSKDELVFVHG
                     ALKALAADLMKIANDIRWLASGPRCGLGELVIPENEPGSSIMPGKVNPTQCEAVTMVA
                     VQVMGN"
     gene            complement(1115..2653)
                     /locus_tag="PK849_10030"
     CDS             complement(1115..2653)
                     /locus_tag="PK849_10030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPE66503.1"
                     /translation="MTFDIKTVVSLWLLLSVISALTVVSMYFRYRERFQGIAFWALHM
                     GLYTAGVLLLVLRGGVPDVLSVLLANYLLILSLFFLLNGMERFVGRPGRHFHNYALVA
                     VYALAFWYFFDVRPDMTLRTVLTSLTASAFFGQVTFLLLIRTPSNLRDITRTSAFVMI
                     SYAVLSLARALLLIFSPLETNEFFEAGGLTSVALLCYVGLSVCWLVAIILMIPRRLLE
                     EVSVQEEKFAKIFRSSPGAIMLTRKADGRIFDVNGGFLEMTGYAFEEIVGATVFATKL
                     WGDDKEYADIFSALSRDQGVRGIESVLRKKSGESMSVLFSSDIVVIDGEQCMLSTFSD
                     ITEQSRLRRQLHEMATHDPLTGLPNRRLFDERATDVFARATRQKRKFAIMSFDIDHFK
                     DINDTYGHAVGDEVLRQAAVRLKESLRITDTVARLGGDEFVALIEEVRGRSDASRVAF
                     KLLELFRRPFIVEGHEVLIRVSIGVALYPENGETVEVLLKKSDDALYTAKEMGRDTLK
                     MAED"
     gene            complement(2677..>3408)
                     /locus_tag="PK849_10035"
     CDS             complement(2677..>3408)
                     /locus_tag="PK849_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPE66504.1"
                     /translation="VALVRRSFSGGGKWPFLPTLIAVLATAAYQPFFFTGTAKTGVAV
                     GTLVTLASAPVVSGIVGFFALGERPQRVWYLSSLLAIGGCSLLTLSGDAESVVHPVGV
                     LFSLGAGFSYAIFVLGSKYLLKEHTPDAVNGVVFSGAGLVLFPVLLMTPPMWLAEPRG
                     LLVALHLGLIATGLAYFLFLKGLSSVPASTSVTLSMAEPLTASLLGVFLLGERMNFLG
                     GIGVFLTFAGVALLALGGSRSAGDS"
BASE COUNT          828 a         1010 c          886 g          684 t
ORIGIN      
        1 gttgcccatc acctggacgg ccaccatcgt aacggcctcg cactgcgtcg ggttcacttt
       61 ccccggcata atcgaactcc ccggttcatt ctcaggaatg acgagttctc cgagaccgca
      121 tcgcggaccg gaagcgagcc agcgaatgtc gttggcgatt ttcatgagat ccgccgcgag
      181 cgccttcagc gcgccgtgga caaacaccag ttcatctttg cttgtcaacg aatgaaattt
      241 gttcggcgcc gttcggaact cttttcccga aagcatggaa agctcctccg caacagaagc
      301 ggcaaaatca gggtgagcgt tcagtccggt tccgacagcc gttccgccga gcgccaactc
      361 tctcaagaat tcgagtccgg agcgaatcat ctcttcgtct ctttcgagca tacgagccca
      421 tccgcttatc tcctgcccaa gcgtaagggg agtagcgtcc tgcaggtgcg tccttcctat
      481 cttcacgacg ccggcgaact gagtcgcctt tttgccgagc gtcgaccgaa gactttcaag
      541 agtcggaagc agcgaatcct ccaatgccaa taccgccgcg acgtgcatcg ccgttgggaa
      601 ggtgtcattg gagctttgcg agcgattcac gtgatcgttg gggtgaatga ccgtctcccc
      661 gccgcaaagt tccgtggcgc gccgcgcgat cacttcgttg acgttcatat tcgtctgggt
      721 tccgctgccg gtctgccaga cgacgagggg gaactgatcg tcatgcttcc tctccaggat
      781 ttcgtcggcc gctccgcaaa tagcccgcat acgtacttcg tcaagcagac cgagacgggc
      841 gttcgttctc gcggcggctt ttttcagcag agcaaatgcg gcgatgatct cctcaggcat
      901 tttttccgcg cctattctaa agttctgcaa acttctctgg gtctgagcgc cccacagttt
      961 tgaagcaggt acctgcactt ctcccagaga gtccttctcc attcgaaacc cctgatccat
     1021 aagcataccc tccttgcgcg gcttcacgct cttttttcgt ttcgcgccca ttgtaccgtt
     1081 caccccaaaa gaacccaaaa gcatccttgt tttttcagtc ctccgccatt ttcaaagtgt
     1141 cccgccccat ctcctttgcg gtgtagagcg catcgtcgga cttttttaaa aggacttcaa
     1201 cagtttcgcc attttccgga taaagcgcaa cgcctatact caccctgatg agcacctcat
     1261 gcccctccac aatgaacggc cttcggaaaa gctccaggag tttgaaggcc acccttgacg
     1321 cgtcgcttcg tccacgaacc tcctctatca aagcgacgaa ctcgtcgccg ccaaggcggg
     1381 caacggtatc ggttatacgc agagattctt tcagacgaac agctgcctgc cgtaacactt
     1441 catctccgac tgcgtgtccg taggtatcgt taatatcctt gaagtggtcg atgtcgaagg
     1501 acattatggc aaacttgcgt ttttgcctcg tcgcacgtgc gaagacgtcg gttgcccgtt
     1561 catcgaagag cctgcgattg ggcagtcctg tcaggggatc atgcgtggcc atttcgtgca
     1621 gctgccgccg cagccggctc tgctccgtta tatccgagaa agtcgacagc atacactgct
     1681 cgccgtcgat cacaacaata tccgaagaga aaagaacact catgctctct cccgattttt
     1741 tccggaggac actctcgatc ccccgtacgc cctggtctct tgacaaggca gagaaaatat
     1801 cggcatattc cttgtcatct ccccacagtt tggttgcaaa gacagtggcg cctacaatct
     1861 cttcgaacgc atatcctgtc atctcaagaa aaccgccgtt cacgtcgaaa attctcccgt
     1921 ccgcttttct cgtaagcatg atcgcgccag gggatgaacg gaatatcttc gcgaactttt
     1981 cctcttggac actgacctct tcgagcaggc ggcggggaat catcaggata atcgcgacga
     2041 gccaacagac gctcagaccg acataacaga gcagcgcaac tgaagtgagc cctccagctt
     2101 cgaagaattc gttcgtttcc aggggagaaa aaatgaggag aagcgcccgg gcaaggctca
     2161 acactgcata agaaatcatg acgaacgcgc tcgtcctggt aatatcgcgc agattcgaag
     2221 gcgttcgtat cagcagaagg aaagtaacct ggccaaaaaa cgcagaggct gtgagcgacg
     2281 tcagcacagt acggagcgtc atatccggcc ggacgtcgaa aaagtaccag aaagccagag
     2341 catacaccgc taccagagcg tagttatgga aatgacgtcc cggtcttccg acaaatcgtt
     2401 ccattccatt cagaagaaaa aacagggaga ggataagaag ataattggca agaagtactg
     2461 aaagaacatc cggaacgccg ccgcgaagaa ccagcagcaa cacaccggct gtgtacagcc
     2521 ccatatggag agcccaaaaa gcaattcctt gaaaacgttc gcgataccgg aagtacatgg
     2581 aaacaaccgt gagagcgcta attacgctca agaggagcca taacgacact acggtcttga
     2641 tgtcaaacgt catgggaacc tccgcttcgt cccgagtcag gaatctcctg cactgcggct
     2701 tcctccgagc gcaagcagcg ccaccccggc gaatgtgagg aagacgccga ttccaccgag
     2761 gaagttcatt cgctcgccaa gcagaaagac cccgagcaga gaagcggtca gcggctccgc
     2821 catggagagc gtgacgctgg tcgaggcggg aacggaggag agtcctttaa gaaagagaaa
     2881 atacgcgagc ccggtggcaa tgagccccag gtgaagcgcg acaagcaatc cccgcggttc
     2941 ggcaagccac atcggggggg tcatgagaag cacaggaaac agcacaagcc ccgctccaga
     3001 aaagaccacc ccgttgacgg cgtcgggggt gtgttccttc aacaggtact tgctgccgag
     3061 gacaaagatg gcataggaga accccgcccc gagagagaaa agcacgccga caggatgcac
     3121 aacgctttcg gcgtctccgg acaaggtcag aagtgagcat cccccgatgg caaggagact
     3181 tgaaaggtac cacactctct gcggccgctc accgagagcg aagaaaccga cgatgcccga
     3241 gacgaccggc gcgctggcga gcgtgaccag cgttccaaca gcaacccctg tcttcgccgt
     3301 tcctgtgaag aaaaagggct ggtatgccgc cgtcgcaagc acggcgatga gagtggggag
     3361 aaacggccac ttgccgcccc ctgagaacga acgccgtaca agcgcaac
//
LOCUS       DAOPKU010000322         3406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_31119_length_3406_cov_4.347061, whole genome shotgun sequence.
ACCESSION   DAOPKU010000322 DAOPKU010000000
VERSION     DAOPKU010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31119_length_3406_cov_4.347061"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2042
                     /locus_tag="PK849_10040"
     CDS             <1..2042
                     /locus_tag="PK849_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPE66505.1"
                     /translation="TNDDDREDLAEPVHPLTRQMNRFGKVLSVVVVGLAGLMMLAGRT
                     LHDFAWGDLFFAAIGFAVAAIPEGLPAILTITLALGVQRMARRNAITRKLNAVETLGS
                     VTVICSDKTGTLTRNEMTVRYAITPEGKYEVSGTGYRPEGEISLDGSAVSAEERDDLN
                     ALVETMAVCNDSEIVEEDGEWRVTGEPTEGALRTLARKASFDPARYERIASIPFESEN
                     KFMATLERLPGGGVRILFKGAPDRLLERCGQQRGADGTVQPLDRKSWEQRMEELGGEG
                     LRVLAAAFKDADEGKSGLDMEDVENDMVFLGLVGIIDPPRPEAIEAIKICRDAGIKVK
                     MITGDHAGTASAIGREMGITGTGLAVTGEELEKASDEELRTIVRDADIFARTSPEHKL
                     RLVQALQANGEVVAMTGDGVNDAPALKRADVGVAMGIKGTEATKEAADIVLADDNFIS
                     IERAVEEGRTIYDNLRKAILFILPTNGAEALVILAAVVFGLSLPLTPVQILWVNMVTA
                     VTLALALAFEPAEPGVMQRPPRSPDAPILGGLFLWRIAFVSVLIGGATIAVFLVERRL
                     GMPLETARTLAVNTLVCGQAFYLFNSRYLRESSLAPDRVFANRAAWIAVGVLAVLQIV
                     FVYAPFMQLWFGSAPLELRHWLIPLSLGAAVFIVVEAEKNVTRRVRAANGRRSGV"
     gene            2089..2982
                     /gene="otsB"
                     /locus_tag="PK849_10045"
     CDS             2089..2982
                     /gene="otsB"
                     /locus_tag="PK849_10045"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HPE66506.1"
                     /translation="MKKKKIDSPAGAGFNAPPGNGERCAAEEILQTGDPLPSALDGFD
                     AIARKAEGKRIAIFLDYDGTLSPIVETPEQAIMAADMREAVVKLAECTPLGIISGRDL
                     EDVRDKVKIDGIVYAGSHGFDITGPDGLKFENTVGEDFLPALDSAENALSEKIGAIEG
                     LFLERKKFAIAIHYRRVAPENIERIEAAVDETTSDHPDLRKAYGKKIFELQPKADWHK
                     GKALFSLMRTLELDRDDVLLVFIGDDATDEDAFRALQGRGVGIVVREKAYETAAAYSL
                     KDPDEVREFLLRLIPLCRGSS"
     gene            complement(3145..>3406)
                     /locus_tag="PK849_10050"
     CDS             complement(3145..>3406)
                     /locus_tag="PK849_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66507.1"
                     /translation="PAGIINKVAWGPKPVDFSLDPLLSKGATLQGSFSHSWDIWEKVL
                     VIMGQGQMPLDALITHELPLEGWKEGFDLIESRKGLKVVLKP"
BASE COUNT          661 a          992 c         1138 g          615 t
ORIGIN      
        1 agaccaacga cgacgaccga gaggaccttg ccgaacctgt tcatccgctt accaggcaga
       61 tgaacaggtt cggcaaggtc ctctcggtcg tcgtcgttgg tctggcgggg ttgatgatgc
      121 tggccggtcg gacgcttcac gactttgcat ggggggatct cttcttcgcg gccatcggat
      181 ttgccgtcgc ggccatcccc gaagggctgc ccgccattct gacgattacg ctcgcgctcg
      241 gcgttcagcg gatggcccgc cggaacgcga tcaccagaaa gctgaacgcc gtcgagacgc
      301 tcggctccgt gacggtgatc tgctcggaca agacaggcac gctcacccgg aacgagatga
      361 cggtgcgcta cgcgatcaca cccgagggga aatacgaagt ctcggggaca ggataccgtc
      421 ccgaggggga gatctcgctc gacgggagcg cggtctcggc cgaagaacgc gatgacctca
      481 acgccctggt ggagacgatg gctgtctgca acgactcgga gatcgtcgag gaggacggag
      541 agtggcgagt gaccggcgag cccacggaag gggcgctgcg taccttggcg cgcaaggcgt
      601 cgttcgatcc ggcccggtac gagcggatcg catccattcc cttcgagtcg gagaacaagt
      661 tcatggcgac gctcgaacgg cttcccggag gaggcgtcag gatcctgttc aagggcgccc
      721 cggataggct gctggaaagg tgcggacagc agaggggcgc ggacggaact gttcagccgc
      781 tggaccggaa gtcgtgggaa cagcggatgg aggagctggg cggcgaaggg cttcgcgtgc
      841 ttgccgccgc cttcaaagat gcggacgagg gaaagagcgg gctggatatg gaggatgtcg
      901 aaaacgacat ggtgttcctt ggcctcgtcg gcattatcga cccgcctcgt ccggaggcga
      961 tagaggccat caagatatgc agggacgcgg gaatcaaggt gaagatgatc accggcgacc
     1021 atgcggggac cgcaagcgcc atcggacgcg agatgggcat taccggtacg ggccttgccg
     1081 ttaccggcga ggaactggag aaggcttccg acgaagagct gcgcacgatc gtacgggacg
     1141 cggacatctt tgcccgcacc agcccggagc acaagctgcg gcttgtgcag gcgcttcagg
     1201 cgaacggcga agttgtcgcg atgaccggcg acggcgtgaa cgacgctcct gcgctcaagc
     1261 gggccgacgt cggcgtggcc atggggatca aagggacgga ggcgaccaag gaggcggcgg
     1321 acatcgtgct ggcggacgac aacttcatct ccatcgagcg cgcggtggag gaggggcgca
     1381 ccatctacga caatctgcgg aaggccattc tcttcatcct gccgacgaac ggcgccgagg
     1441 cgctggtcat tcttgcagcc gtggtcttcg ggctgtccct tccgctgacg cccgtgcaga
     1501 tcctctgggt caacatggtg accgccgtca cgctcgccct cgctcttgcc ttcgagccgg
     1561 ccgagccggg agtgatgcag cgccctccgc gctcgcccga cgccccgatt ctcggcggac
     1621 tcttcctgtg gcgcatcgcg ttcgtctccg tgctgatcgg gggggcgaca atcgccgtct
     1681 tccttgtgga acgccggctc ggaatgccgc tggagacggc gcggaccctc gcggtcaata
     1741 cgctcgtctg cggacaggcg ttctatcttt tcaacagccg gtatctccgg gagtcgagtc
     1801 ttgcgcccga cagggttttc gcaaaccgcg ccgcgtggat agcggtaggc gtgctggccg
     1861 tactccagat cgtcttcgtc tacgcgccgt ttatgcagct ctggttcggc tcggcgccgc
     1921 tggagcttcg ccactggctg attcccctct ctctgggcgc ggcggtcttc atcgtcgtcg
     1981 aagcggaaaa gaacgtgacg cgccgtgtgc gcgccgcgaa cggacgccgg agcggcgtgt
     2041 agtccgaaca ggtattggat gagatgttga agaagcggag gtatagaaat gaaaaaaaag
     2101 aaaatcgact ccccggcggg ggctggtttc aacgcgccac cggggaacgg agagaggtgc
     2161 gcggccgagg agatccttca aaccggcgat ccgcttccct ccgctctgga cggtttcgac
     2221 gcgatcgccc gcaaggcgga gggaaagcgg attgcgatct tccttgatta cgacggcacg
     2281 ctctctccga tcgtggagac tccggagcag gcgattatgg ccgcggatat gcgggaggcg
     2341 gtcgtcaagc tcgccgaatg tactccgctc gggatcatca gcggccggga tctcgaagat
     2401 gtgcgcgaca aggtgaagat cgacggcatc gtctacgcgg gaagccacgg tttcgacatc
     2461 acgggccccg acggactaaa gttcgaaaat actgtagggg aggattttct ccccgcgctc
     2521 gacagcgcgg agaacgccct gtcggagaag atcggcgcca ttgaagggct ttttttggag
     2581 agaaaaaagt tcgccatcgc catccactac cggcgggttg ctccagaaaa catcgagcgg
     2641 atagaggccg ccgtcgacga aacgacgtcg gaccaccccg atctcaggaa agcctacggc
     2701 aagaagatat tcgagctcca gccgaaggcg gactggcaca aggggaaggc gctcttctcg
     2761 ctgatgcgta cgctggaact cgaccgggac gacgtgctcc ttgtctttat cggagacgac
     2821 gcgaccgacg aggacgcgtt ccgcgcgttg cagggaagag gcgtcggcat cgtggtccgg
     2881 gaaaaagcct acgagaccgc cgctgcatac agcctgaagg atcccgatga agtccgggag
     2941 tttcttctcc gtttgattcc gttgtgcagg gggtcgtcat gatggcgtgg gatgtgctcg
     3001 cacagaagag ccattgtctt cggggccttg ttcgggaaat cgtcggaaac acggaacagc
     3061 gatatcctgc gaatggactc tcttgaattt tgaaagggag gacgtgcgaa cgccctccct
     3121 ccaaaaaaat agctttttca gccgctacgg cttgagcacg accttcagcc ctttgcggct
     3181 ttcgatgagg tcgaatccct ctttccaccc ctcgagcggg agctcgtggg tgatgagggc
     3241 gtcgagcggc atctgcccct gtcccatgat gacgagcacc ttctcccaga tgtcccacga
     3301 gtggctgaag gacccctgga gtgtcgcgcc cttcgagagc agcgggtcga ggctgaaatc
     3361 cacgggtttc ggcccccacg ctaccttgtt gatgattcct gcgggc
//
LOCUS       DAOPKU010000323         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_31355_length_3388_cov_4.963696, whole genome shotgun sequence.
ACCESSION   DAOPKU010000323 DAOPKU010000000
VERSION     DAOPKU010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31355_length_3388_cov_4.963696"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PK849_10055"
     CDS             <1..814
                     /locus_tag="PK849_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66508.1"
                     /translation="FQEEQIPLVAAYAIHPDVTTAGDFCFRNGFLGFVEGKAAGYASH
                     KLLGAKTVALLTSDNDFGRTLVTGFKEYAAKYAPDLKIVSEQAYPFSEKDYKPYLSKI
                     KELNPDVIFASGYYFQTGPLLRQARELGIESKILGEEGADSPKLVEIAGEAAEGFHIV
                     TNFDRDDPRPVVQNFLKEFRGRHKFEPDMVGASAYDAFMIIVNAMKTAGTTDGKPVRD
                     AIAATVGYDGLTGVMGGFNEIGEVVKPVQVQVVKEGLFRHFGVVDDPELIKP"
     gene            1225..2586
                     /locus_tag="PK849_10060"
     CDS             1225..2586
                     /locus_tag="PK849_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HPE66509.1"
                     /translation="MKLSVLKNFKPGPIEADFLNMLMFDIGGGMRSVSLPRGYVSEAI
                     FRDGIGFDASNYGFAKVDQSDMVAIPDCNAAWLEERGDFSIVHVLCDVVSTERKVFDQ
                     YPRSVAERTAEYLKEKGLADSAQMLVELEYYVFDSVEYSTGLDHAFYSIGSGEGLGED
                     FNTQQRFAPLKGYHKLPPEDRFFDFRNETVRLMEEAGIAVKYHHHEVGASQLEIELDF
                     MDLVKAADSVCIAKWIIHSVAEEMGFKVTFMPKPLYKMPGSGMHVHQFLEKGGKSLFP
                     GGELHGLSKEGLAYTSGLLEHSLTGSLLAFTNPSTNSFRRLVPGYEAPISATFAKGSR
                     SAAVRIPGYLKKDEVRVEYRTGDASANIHYMLSAMVLAGVDGILRNADPVANGFSSAE
                     AKQEKVFPLSLNAVLDGLKKDNEWLKPAFPEKLMELWTKRKLEEAEYVYNAPTPQEYE
                     LYF"
     gene            2864..3103
                     /locus_tag="PK849_10065"
     CDS             2864..3103
                     /locus_tag="PK849_10065"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HPE66510.1"
                     /translation="MATLEKTLTVRLTPEERMAVEEYAKEKNMTIAQLARESLLEKIE
                     DAYDLEVYTAWLKSKRETVRFEDLVKECGFSEEDL"
     gene            3100..3378
                     /locus_tag="PK849_10070"
     CDS             3100..3378
                     /locus_tag="PK849_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPE66511.1"
                     /translation="MSVWSVEIDAGTAKKIQKLDRETQRLILSYIDRHLRNGTDPRAS
                     GKALTGPLRGLWRYRIGDYRLLAEIRDDELIIVAVDIGHRSAIYSRRR"
BASE COUNT          726 a          993 c         1043 g          626 t
ORIGIN      
        1 cttccaggag gagcagatcc ctctggtggc ggcgtacgcc attcacccgg acgtaacaac
       61 ggccggcgac ttctgcttcc ggaacggctt cctcggcttc gtcgagggga aggccgccgg
      121 gtacgcctcg cacaagctgc tgggcgcgaa gacggtcgcc ctgctcacca gcgacaacga
      181 cttcgggcgg acgctcgtga ccggcttcaa ggagtacgcc gcgaagtacg ctcccgatct
      241 gaagatcgtc agcgagcagg cctacccctt ctcggagaag gactacaagc cctatctctc
      301 gaagatcaag gagctgaacc ccgacgttat cttcgccagc ggctactact tccagaccgg
      361 cccgctgctt cggcaggccc gtgaactcgg catcgagtcc aaaatcctcg gcgaagaggg
      421 cgccgactcg cccaagctcg tcgagatcgc gggcgaggcc gccgagggct tccatatcgt
      481 caccaacttc gaccgcgacg acccccgtcc cgtggtccag aacttcctga aggagttccg
      541 cggccgccac aagttcgagc ccgacatggt cggcgcttcg gcgtacgacg ccttcatgat
      601 catcgtcaac gccatgaaga cggcgggaac gaccgacggc aagcccgtgc gcgacgccat
      661 cgccgcgacc gtggggtacg acggcctcac cggcgtcatg ggcggcttca acgagatcgg
      721 cgaagtggtc aagcccgtgc aggtgcaggt ggtcaaggag gggctcttcc gccacttcgg
      781 cgtggtcgac gatcccgagc tgatcaagcc gtaagcggaa acgaaacgat agatacgctc
      841 tgcaaaagca aaagcctctc cttcggggga ggcttttttc ataggctccg ggaaaaaagc
      901 gtttttttcg cgacaaaaaa tcagcgttta tggtacaata aattccgctc agtgaaaaac
      961 gacagtaaaa tataccaaac ataaagtatc tcaatgtaaa gaatacagcc tttgcccgaa
     1021 aacagcgggg ataggcttca tacgggcttt cgcgcaacgc ctctccctcg ggggaggctt
     1081 tttgcacagt ctcccggcga ggggcctcac gccttgcgcc gacgaaattc ggggctgtcc
     1141 ggaagcgatt tgggaagcgt ttttagttca cggagggcgc ggcgcgtttt ccgaacaata
     1201 atacagcgat atggaggaat acgaatgaaa ctgtcggtac tcaagaattt caagcccggt
     1261 cccattgaag cggattttct gaacatgctg atgttcgaca tcggcggcgg catgcgctcg
     1321 gtgagtcttc cgagagggta cgtctccgaa gcgatcttcc gcgacggcat cggcttcgac
     1381 gcgtccaact acggcttcgc caaggtcgac cagtccgaca tggtcgccat cccggactgc
     1441 aacgcggcgt ggctggaaga gcgcggcgac ttcagcatcg tccacgtgct ctgcgacgtc
     1501 gtctccaccg agcggaaggt cttcgaccag taccctcgtt ccgtcgcgga gcgcaccgcg
     1561 gagtacctga aggagaaggg gctcgccgac agcgcgcaga tgctcgtcga gcttgagtac
     1621 tacgtcttcg actccgtcga atactccacg gggctcgacc acgccttcta ctccatcggt
     1681 tcgggcgaag ggctcggcga ggacttcaac acgcagcagc gtttcgcccc gctgaagggg
     1741 taccacaagc tgccgcccga ggaccgcttc ttcgacttcc ggaacgagac cgtccggctg
     1801 atggaagagg cgggcatcgc ggtgaagtac caccaccacg aggtgggcgc gtcgcagctc
     1861 gagatcgagc tggacttcat ggacctggtg aaggccgccg acagcgtctg catcgccaag
     1921 tggatcatcc actcggtcgc cgaggagatg ggcttcaagg tcaccttcat gccgaagccg
     1981 ctctacaaga tgcccggcag cggcatgcac gtccaccagt tcctcgaaaa gggcgggaag
     2041 agcctctttc cgggcggcga actccacggt ctgtcgaagg aggggcttgc ctacacctcc
     2101 ggcctgctgg agcacagtct cactgggagc ctgctcgcct tcacgaaccc cagcacgaac
     2161 agcttccggc ggctcgtccc cggctacgag gcccccatca gcgcgacctt cgcgaagggg
     2221 tcgcggagcg cggcggtgcg catccccggc tacctgaaga aggacgaagt ccgcgtggag
     2281 taccgcaccg gcgacgcctc ggcgaacatt cactatatgc tctcggcgat ggtgctcgcc
     2341 ggagtcgacg gcattctccg gaacgccgac ccggtggcga acggcttcag cagcgcggag
     2401 gcgaagcagg agaaggtctt cccgctgagc ctgaacgcgg tgctcgacgg cctgaaaaag
     2461 gacaacgaat ggctgaagcc cgccttcccc gaaaagctga tggagctgtg gacgaagcgg
     2521 aagcttgagg aggccgagta cgtctacaac gccccgacgc cgcaggagta cgagctgtac
     2581 ttctagggcg gggcgcggtt cgaagacgcg agcgggaggg gcttcggccg gagcggggac
     2641 gcttcgccgg agcctctccc gttttgtgtt cgccgcttcc gcggcggtac ggaaatttcg
     2701 tgcgggggtg tccggcgtga tggcaacgtc gcaggatgca acgatttctt ccggtttttg
     2761 aatttcttcg atttctttca tataagtttt taatgagggc aactgtccgg agttgacagc
     2821 gcataccttt gtgttacgat gcaatacaaa ggaggtggct gaaatggcga ctttggaaaa
     2881 gacactcacg gtacggctca ctcccgaaga gcgtatggcg gtcgaggagt acgcgaaaga
     2941 gaaaaatatg accatcgccc agctcgcccg ggagtctctt ctcgaaaaaa tcgaggatgc
     3001 ctatgatctc gaagtatata cggcatggtt gaagagcaag cgagagacgg tgcgcttcga
     3061 ggatttagtg aaggagtgcg ggttttccga ggaggattta tgagcgtctg gtcggtagaa
     3121 atagatgccg ggacggcgaa gaagatacaa aaactcgatc gagagacgca acgtcttatt
     3181 ctgagctaca tcgaccgaca tcttcggaac ggcaccgatc cgcgagcttc cgggaaagct
     3241 ctcacgggtc cgctgcgcgg actatggcgc tacaggatcg gagactaccg tctgcttgcc
     3301 gagatacggg atgacgaact catcatcgtc gccgtggata taggtcatcg ttcggcgatc
     3361 tattcccgaa ggagataggc tgcgtact
//
LOCUS       DAOPKU010000324         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_31356_length_3388_cov_4.816082, whole genome shotgun sequence.
ACCESSION   DAOPKU010000324 DAOPKU010000000
VERSION     DAOPKU010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31356_length_3388_cov_4.816082"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK849_10075"
     CDS             complement(<1..342)
                     /locus_tag="PK849_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66512.1"
                     /translation="MFRVSYKPSTSHWLFPPIILGILALLLAVMIAQRAIECRRTGRP
                     FINREAMRFFEPGCDKLKLFGSVALFVLYIYAMEFAGFLASSIVVVFLYSVLFFGVDR
                     LKELPAAIASGR"
     gene            complement(426..1445)
                     /locus_tag="PK849_10080"
     CDS             complement(426..1445)
                     /locus_tag="PK849_10080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HPE66513.1"
                     /translation="MKFRKALLALLVLVLLAVPAVAGANPFAGMNVRVVIGSTSTAGD
                     SYLIAETVSRHLAKALGANMKVDAVGAAVALDAMQTSRPDGKTIMMFHDMTYLGVSFK
                     AYDDIYKLENMEIGPRVAQNPGAAWAAYLDAPYNTMAEIPEYLKANPEAKVRMACEAG
                     GVSHIGFIAFWEWVESKYGKDVADRIVVIVGGSTSDKIQLLIDRNADVIFADYTSLLD
                     YTKVGDKKIAMKFVGLLDNVEGVDVPSYADQGITLADGSEFRFSKDFLIYLPKNFPAE
                     MVKALDDGMKAVSEDPQFKADMAKMSYRGGYLDSAAAKKFIYQKRDSLQGLIDSAPSL
                     DDLVM"
     gene            complement(1503..2840)
                     /locus_tag="PK849_10085"
     CDS             complement(1503..2840)
                     /locus_tag="PK849_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HPE66514.1"
                     /translation="MKKTCQPVVTEMRVIPVAGRDSMLLNLSGAHGPYFTRNVVLLTD
                     SEGRTGVGEVPGGEKIAGGLEQARDLVVGSRTGEYKNTLLAVKKLFVGREDDVRGQQT
                     FDLRTGVHVLTGIEAPLLDLLGQYLGVPAASLLGDGQLRERIPVLGYLFYVADRKKTD
                     LPYISESGSSVEWYRLRREEATTPEAIVALAKASRDLYGFSDFKLKGGVFPGSEEMEA
                     VRTLKRAFPDGRITIDPNGGWLLKEAVELCRGMHGILSYCEDPCGAEGGFSGREVMAE
                     FRRATGLPTATNMIATDWRQMAHSAMLQSVDIPLADPHFWTMEGSVRVGQFCEVFGLT
                     WGSHSNNHFDISLAMFSHTAAAVPGRITAVDTHWIWQEGTERLTKEPLTIRDGFIDLP
                     AAPGLGIEPDMEAIEAAHRLYMEHCLGARDDAAAMQFLIPGWKFDPKRPCMVR"
     gene            complement(2869..>3388)
                     /locus_tag="PK849_10090"
     CDS             complement(2869..>3388)
                     /locus_tag="PK849_10090"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE66515.1"
                     /translation="SLLDVDDIEEGRYIRKCLESDTLIQACRSFSADGAIALEANLKM
                     QQNALDTGDRKRFLFLDQDFHRLICSGCGRERVWSVISKNSLHFFRVRRLKIKGGGVG
                     QGYFHEQHGELLDAILAHETDRALDILNRHLTWDVETVRASYPDYFSRDSMSKKMYMF
                     MSEVAPVVAGAM"
BASE COUNT          641 a         1117 c         1010 g          620 t
ORIGIN      
        1 cctccccgaa gcgatggccg ccggaagctc tttgaggcgg tcgacgccga aaaacagcac
       61 gctgtacaga aagacgacga cgatgctcga cgccagaaac ccggcgaact ccatcgcgta
      121 gatatacaag acgaagagcg ccaccgagcc gaacagcttg agtttgtcgc agccgggctc
      181 gaagaagcgc attgcttccc tgttgataaa aggccgccca gtcctgcggc attcgatcgc
      241 ccgctgcgcg atcatcaccg cgaggagcag cgccagaatg cccaggatga tgggcggaaa
      301 cagccagtgg gacgtggaag gtttgtaact cactctgaac atcgttcaat cctccggagg
      361 cagtatcgaa agggagcggc gcgggaaacg aaaagcgttc tccgcgccgc tccgtccggg
      421 aagcgctaca tcacaaggtc gtcgagggac ggggcggaat cgatcagccc ctgcagggaa
      481 tctctcttct gatagatgaa cttcttcgcc gccgccgagt cgaggtaccc gccgcggtag
      541 gacatcttcg ccatgtccgc cttgaactgc ggatcctcgg agaccgcctt catgccatcg
      601 tcgagcgcct tgaccatctc ggccgggaag ttcttcggaa ggtagatgag gaaatccttg
      661 gagaagcgga actcgctccc gtcggcaagc gtgatcccct ggtcggcgta cgagggaacg
      721 tcgacgccct ccacgttgtc cagaaggccg acgaacttca tggcgatctt cttgtccccg
      781 accttggtat agtcaaggag agatgtgtag tcggcgaaga tcacgtccgc gttccggtcg
      841 atgagaagct gaatcttgtc ggaggtggag ccgcccacga tcacgacgat cctgtccgcg
      901 acgtccttgc cgtacttgct ctcgacccac tcccagaagg cgatgaaacc gatgtgagaa
      961 acgccgcccg cttcgcacgc catgcggacc ttcgcttcgg gattcgcctt caggtactcg
     1021 gggatctccg ccatggtgtt gtacggggcg tccagatacg ccgcccatgc ggcgccgggg
     1081 ttctgcgcga cccgggggcc tatctccatg ttctccagct tgtagatgtc gtcgtaggcc
     1141 ttgaaggaga cgccgaggta ggtcatgtcg tggaacatca tgatcgtctt cccgtcgggg
     1201 cgcgaggtct gcatcgcgtc gagcgccacc gccgcgccga ccgcgtcgac cttcatgttc
     1261 gcgcccagcg ccttcgcaag gtgccgggag acagtctcgg cgatcaggta cgaatcgccg
     1321 gcggtcgagg tggacccgat gaccacgcgg acgttcatcc cggcgaacgg attcgccccc
     1381 gcgacggcgg gaacagccaa cagcacgagt accaacagag caagcagagc ctttctgaac
     1441 ttcatgtgaa ctcctccttt tgttccggag cgcaggcccc ggtttttagt gctccaagcg
     1501 ccctagcgaa ccatgcaggg gcgcttcgga tcgaatttcc agcccggaat aaggaactgc
     1561 atcgccgccg cgtcgtcccg cgcacccaga cagtgctcca tgtagagccg gtgcgccgcc
     1621 tcgatcgcct ccatatccgg ctcgatcccc aggccgggag ccgcggggag gtcgatgaat
     1681 ccgtcccgga ttgtgagagg ctccttcgtg aggcgttccg tcccctcctg ccatatccag
     1741 tgcgtgtcga cggccgttat cctgcccgga accgcggcgg cggtgtggct gaacatggcc
     1801 agagagatat cgaagtggtt gttcgagtga gagccccacg tgaggccgaa gacctcgcag
     1861 aactgcccca cccgcaccga accctccatc gtccagaagt gagggtcggc cagcggaatg
     1921 tccacggact ggagcatcgc cgagtgggcc atctgccgcc agtcggtggc gatcatgttc
     1981 gtcgcggtag gcagccccgt ggcgcggcgg aactccgcca tgacctcgcg ccccgaaaag
     2041 cctccctcgg ccccgcacgg gtcctcgcag taggagagga taccgtgcat ccctctgcac
     2101 agttcgacag cctccttcag cagccaccct ccgttggggt cgatggtaat ccgcccgtcg
     2161 gggaacgcgc gcttcagcgt cctcacggcc tccatctcct cgcttccggg gaagacgccc
     2221 cccttgagct tgaaatccga aaaaccgtaa agatcgcggc tcgcctttgc aagcgcgacg
     2281 atagcctccg gcgtcgtcgc ctcttcgcgg cggagcctgt accactcgac ggaggaaccg
     2341 gactccgaga tgtacgggag atcggtcttc ttcctgtccg ccacgtagaa gaggtacccc
     2401 aggacgggaa tcctctcccg gagctgcccg tcgccgagga gcgacgccgc gggcacgccg
     2461 agatactgcc ccagcagatc gagcagcggc gcctcgatgc cggtgagtac gtgcacccct
     2521 gtcctcaggt cgaatgtctg ctgtccccgc acatcgtcct cccgccccac gaagagcttc
     2581 ttcacggcga gcagcgtgtt cttgtactcc cccgtccgtg agccgacgac gaggtcgcgg
     2641 gcctgttcga gccccccggc gatcttctcg ccccccggaa cctctccgac gcccgtcctc
     2701 ccctcgctgt ccgtcagcag caccacgttg cgcgtgaagt acggaccgtg ggctccgctg
     2761 aggttgagga gcatgctgtc ccgccccgcg acggggatca cccgcatttc cgttacaacc
     2821 ggttggcacg ttttcttcat gctgtcgtcc tttccattcg tcaatgcgct acatcgcacc
     2881 cgcgacgacg ggcgcgactt cggacatgaa catgtacatc ttcttcgaca tggaatcccg
     2941 cgagaaatag tcggggtagg aggcgcgcac cgtctccacg tcccaagtaa ggtgcctgtt
     3001 gaggatatcc agcgcacgat cggtctcgtg cgcgagtatc gcgtcaagaa gctccccgtg
     3061 ctgctcatgg aaatacccct gcccgacgcc gccgcccttg atcttcagcc tgcggacgcg
     3121 aaaaaagtgc agcgaattct tgctgatgac cgaccagact ctttcgcggc cgcacccgct
     3181 gcagatcagc cggtggaaat cctggtcgag aaagagaaaa cgcttgcggt cgcccgtatc
     3241 cagcgcgttc tgctgcatct tcagattggc ttcgagcgcg atcgccccgt cggcggaaaa
     3301 agagcggcac gcctgaatca gcgtgtcgga ctcaagacac ttgcggatat agcgcccctc
     3361 ctcgatatcg tccacgtcga gcagggag
//
LOCUS       DAOPKU010000325         3372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_31561_length_3372_cov_5.324390, whole genome shotgun sequence.
ACCESSION   DAOPKU010000325 DAOPKU010000000
VERSION     DAOPKU010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31561_length_3372_cov_5.324390"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK849_10095"
     CDS             complement(<1..231)
                     /locus_tag="PK849_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HPE66516.1"
                     /translation="MRFWFQTDKTGDRSRVEKLTLAGLFVAAAILLSGVHFPMGPTRC
                     YPFQHTVNVLAGAFLGPWWAAGVAFITSVIRNM"
     gene            complement(206..1144)
                     /locus_tag="PK849_10100"
     CDS             complement(206..1144)
                     /locus_tag="PK849_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66517.1"
                     /translation="MRTKICCIALFFLLLATSAAKGTEKLVLMLDWFPNVDHVPIYAA
                     IESGFFEKRGLFVEIQSPSDSADPLKLAAAGHADIALSYEPQILIAASEGIPLKAVGK
                     LIGTPLTTLLFLETSGIRVPSDLEGKTIGYTVPGMMDHLAEAFASVNAIRNYKLLNVG
                     FSILQSLTSGTADAVMGPFKTYEPVLLEMEGYDSRFFEIDHFGIPTYDELVFVTGDRT
                     YKEKKESIVKFLAAIDEALVFTAAHPSEALALYFRAVPEAPAEMERTAFDRTLPFFAP
                     DTTLSLDQWETFSEFARTCGMIEKKVDLNALLVSDR"
     gene            complement(1160..1924)
                     /locus_tag="PK849_10105"
     CDS             complement(1160..1924)
                     /locus_tag="PK849_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66518.1"
                     /translation="MTGRLIPAAATLSFALCWEAFCRLQEIPHFILPAPSRIAILLLT
                     EAPLLLKHAAVTTFEIALGIVFSLAAALPLSIAMFFSPLLERALSPLLIASQAIPVFA
                     VAPLLVVWFGYGTGSKVIMAALIIFFPVTVTLLQGFKSCDPGYQRLFFVMGADFRKTL
                     RFLYWPWALPYFFAGLRVAVSVAAIGAVIGEWVGSLEGLGYLMMQANARLRVDMVFAC
                     VVLLSCIGLLLWGGVSRLEARYVRWNQDTKEIQTNK"
     gene            complement(1924..2655)
                     /locus_tag="PK849_10110"
     CDS             complement(1924..2655)
                     /locus_tag="PK849_10110"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66519.1"
                     /translation="MLEIRHFSKDFNGLPVLSNFSLALPRNSFTVLVGPSGCGKSTLF
                     DLLMGSVSRDGGRLFLFGAELPDLRGEAGYMAQKDLLLPWLSLIQNALLPVMVKRKLT
                     SDDYKEAKNLFSSLELSGFENHYPRQVSGGMAQRCALARTYFFRAPLALLDEPLSAVD
                     ALTRQSLRTMLLLLQSRFEKTILMVTHDVDDALFLADRIVVLSRSPMMVTQEFIPEGK
                     KPRNPSNTELLHLKEHILRSLQEGR"
     regulatory      complement(2709..2818)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          840 a          886 c          943 g          703 t
ORIGIN      
        1 catatttcgg ataacgctgg tgataaaggc gactcccgca gcccaccatg gaccgagaaa
       61 cgcccccgca agcacgttga ccgtatgttg aaacggatag catcgcgtcg gccccatagg
      121 gaaatgaact cctgaaagaa gaatcgccgc cgccacaaaa agacctgcga gggtcagttt
      181 ttcgacccgg cttctgtctc cggttttatc tgtctgaaac cagaagcgca ttcagatcca
      241 ccttcttttc tatcattccg caggtgcgcg caaattcaga aaacgtctcc cactgatcca
      301 acgaaagcgt tgtatctggc gcaaaaaaag gcagcgtcct gtcaaatgct gttctttcca
      361 tctcggcggg agcttccgga acagcccgga aatagagtgc aagggcttcc gaggggtgag
      421 ccgccgtaaa gacgagcgcc tcgtcgatgg ctgccagaaa cttcacgatg ctctctttct
      481 tttctttgta tgtccgatca ccggtaacga agacgagctc gtcgtatgtc ggtatcccga
      541 agtgatcaat ttcaaaaaaa cgggagtcat agccttccat ttccagcaaa acgggctcgt
      601 acgtcttgaa cgggcccata accgcgtctg ctgttcctga ggttagcgac tgaagtattg
      661 agaaaccgac gttcagcagt ttatagttgc ggattgcgtt caccgaagcg aaagcctctg
      721 caaggtgatc catcatcccc ggaacagtgt agccgatcgt ctttccctca agatcagagg
      781 gaacgcgaat cccgcttgtt tcaagaaaga gaagggttgt caggggagtt ccgatgagct
      841 ttcccacggc tttcagagga ataccctccg aggcggcgat cagtatctgc ggttcgtaag
      901 agagcgcaat gtcggcatga ccggccgccg ccagtttcag agggtcggcg ctgtccgaag
      961 ggctttgtat ttcgacgaag agcccgcgtt tttcgaagaa acccgattca atcgccgcgt
     1021 agatcggcac atggtcaacg ttcgggaacc agtccagcat caggaccagc ttttccgtcc
     1081 cctttgcagc agaggttgcc agcagaagaa aaaacagcgc tatacaacaa atcttggttc
     1141 gcatatacat tcctcccgtt tacttatttg tctgaatttc tttcgtgtct tggttccacc
     1201 ggacatagcg ggcctcaagg cgggaaacgc ccccccagag caggagccct atacacgaaa
     1261 gaagtaccac gcacgcgaag accatgtcga ctcgcaggcg agcgttggcc tgcatcatca
     1321 agtatccgag cccctccaaa gagccgaccc attcgcctat cacagcgcct atagcggcga
     1381 cggagaccgc tacccgcagc cccgcaaaaa aataagggag cgcccacggc cagtaaagaa
     1441 aacgcaacgt ctttcgaaaa tcagctccca taacaaaaaa cagcctttga taccctggat
     1501 cgcagctttt aaaaccttgt agcagcgtta cagtgacagg gaagaaaata atgagcgccg
     1561 ccatgataac cttgctgccc gttccatagc cgaaccagac caccagaagg ggcgccacgg
     1621 cgaagacggg aatcgcctgt gaggctatta acaacggaga aagagctcgt tcaagaagag
     1681 gcgaaaagaa catcgctatt gagaggggaa gagcggccgc aagagaaaat acaatcccaa
     1741 gagcgatctc gaatgtcgtc accgcagcgt gtttcagcag caacggcgcc tccgtcagca
     1801 gaagaatcgc gattcgagag ggggcgggaa gaataaagtg ggggatctcc tgcagacggc
     1861 agaaagcttc ccagcacagc gcaaacgaga gcgttgcagc agcgggaatg aggcggccgg
     1921 tcatcaacgt ccctcctgaa gggagcgcag aatgtgttcc ttcaggtgca ggagttccgt
     1981 attcgacgga ttccgcggct tcttgccttc cggaatgaac tcctgggtta ccatcatcgg
     2041 agagcgagaa agaacgacga tcctgtcggc caggaaaaga gcgtcgtcga catcatgcgt
     2101 gaccatcagg atcgtctttt cgaaacgtga ttgcagcaag agcagcatgg tgcggagcga
     2161 ctgccgggta agcgcgtcaa cagcggaaag aggttcgtcg agaagcgcga ggggagcccg
     2221 gaaaaagtag gtccgtgcaa gagcgcatcg ttgcgccatg cctcccgaaa cttgtcgggg
     2281 gtagtgattc tcaaagcctg agagctccag ggagctgaag aggttttttg cttctttata
     2341 gtcatctgaa gtcagctttc ttttcaccat aacagggaga agcgcgtttt gaataagcga
     2401 aagccatggg agcagcagat ctttttgtgc catgtatccc gcttctccgc gaaggtccgg
     2461 gagttcggcg ccgaaaagaa agagccgccc cccgtctctg gatacgctcc ccatcaggag
     2521 gtcgaagagc gtgctctttc cgcaccctga tgggccgacg agtactgtaa agctgtttcg
     2581 gggcagcgca agagaaaaat tcgaaagaac gggaagcccg ttgaaatctt tagagaaatg
     2641 acgtatctca agcataaaaa actcccggca agggatgccg ggagtaggag ttgcgacttc
     2701 ttcgcggatg gcaacgaacg cactcttccc ttcgccggca tgacccggat caggttccag
     2761 gggtcggggc cgttagcccc ctctcagccg gtagatgccg actcccccag ttgctgtcga
     2821 caacagaata cgcttttccc tttgctttgt caattcattg aaatctgcaa ggaaaagaga
     2881 gataatcgta tgcaggattt gaaaggggga tttcttcgtg aatattcgat gcaaaagctg
     2941 cccgggaagt tttggtgata aagaagttct actgcattat ttcgatgaag agacgagggc
     3001 ggacgcccct ttgttcctct tgctgcacgg agttcacgga tgcgcatctt cggttgaagg
     3061 gaacaagtac gggtacctgg cccgcttgat tgcgcacaag aggttcccgg tatgcgccgc
     3121 agagtccagt cggctgcgac gggatagaga aacgttcggc gacgatcggc ccgcatgggc
     3181 ggtggcttcc ttccagggga aaacgttcgc tatggaggtt ttcgatgcat cctcagccct
     3241 tgctgctgcg gctgcggagt atccaggccg gaaaatagtg ctctggggtt tttctctcgg
     3301 ggggcttgtg tcggtgctcc tcgccgggga gagagcggaa gatttattcg tcagactggc
     3361 ctggagccaa ta
//
LOCUS       DAOPKU010000326         3358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_31759_length_3358_cov_3.101120, whole genome shotgun sequence.
ACCESSION   DAOPKU010000326 DAOPKU010000000
VERSION     DAOPKU010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31759_length_3358_cov_3.101120"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PK849_10115"
     CDS             complement(<1..583)
                     /locus_tag="PK849_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPE66520.1"
                     /translation="MNPVFTLVLAGGSGTRLWPLSREEFPKQFLRIEEEGSLYQCTLR
                     RAFAIGDPSRTKVLSSVRFGAMALLQARELLDVPQNFVVEEPCPKNTAPAIALGLLAL
                     MEECGATRESVVFLCPSDHIIYGPELFQDAVRRAAAAARAGNVCVFGIPPTRPDTGFG
                     YVRAGEPCGEWRKVERFVEKPSLEKAKEYLEAGD"
     gene            complement(742..2133)
                     /locus_tag="PK849_10120"
     CDS             complement(742..2133)
                     /locus_tag="PK849_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPE66521.1"
                     /translation="MAKKIVYGLHDVPPFPILILAGAQHVLTLFGATTLVPLIFGPAM
                     GMSQAQIAVFISCVYFGMGVATLIQTHPALGSGLPIVQGSSFSFIPSVMTIIGAYSAS
                     GPEVIMQYIGGGLIAGGILLSVIGYTGIIGYIRKIITPVVIGPVIMAIGFSLAPTAIQ
                     FNAANYWPVSLLVVALIFFFSLVSKNKYANIFAILSSIVLTYLLCLGLSKAGIFPEGH
                     PAHISLANVEAAPWYRIDIIMPWGTPKFSLLAFGALLAGFFAVMIESVGDYHSVSYAS
                     GLDDPDEKTISRGIGAEGLNCALSGLFGSVGTTSYTENIGLIGLTGVASRWVVRVGAI
                     LLILMSFVTKFGALVATMPSPIIGGAYIALFGTIGALGIQVLMRADMGSQRNVLIVGF
                     AFLMAMGLPGWVEKNQELFMNPAYHQVLQTLGGMVWAILKTPMAVAGICAAFCDSLVP
                     GTDEERGIGVKMK"
     gene            complement(2282..>3358)
                     /locus_tag="PK849_10125"
     CDS             complement(2282..>3358)
                     /locus_tag="PK849_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE66522.1"
                     /translation="IRGKGFALALKAPAMPSDAASSAIVKILGDGTVDVLAATMDMGQ
                     GAYTAYAQMASEELGIPIEKIKCYYPDTQSHPYDWQTVASRSCWSMGMAVKRAATDAR
                     EKLLALYSEYWETEPESITIEHGIVKCKKKGKAEPIGEQIQSGFMMPSGNYVGGPVIG
                     SGSFVPPDVIYPDPETGQSPKSVVHFTVGAVGIDIEVDPGTGAVEVNKVSAGYDVGKA
                     VSPINVKGQIEGGTIQGISAGLMEGMYYDEHGKLLTPDFTDYKIATSMDVPREVDSFW
                     EETPEELSPYGNRGVGEHSMISPAPAIDNALFDALGIRIHTYPLGRERVYKAIQAKKN
                     GETDLWEYPYALEQSYKNAIKEWK"
BASE COUNT          670 a         1021 c         1043 g          624 t
ORIGIN      
        1 agtctcccgc ttcgaggtac tccttcgcct tctccaaaga gggcttctcg acgaagcgct
       61 ccaccttcct ccattcgccg cacggctcgc ccgcccggac gtagccgaaa cccgtgtcgg
      121 gcctcgtggg aggaatgccg aagacacata cgttcccggc tcgcgcggcg gcggcggccc
      181 tccggactgc gtcctgaaac agctcaggtc catagatgat atgatcgctg gggcaaagga
      241 acactacgct ctccctggtc gctccgcact cctccatcag ggcgagcagc ccgagcgcga
      301 tggcaggagc ggtgttcttc ggacagggct cctccacgac gaagttctgc ggaacgtcca
      361 gaagctcacg cgcctgcagg agcgccatcg cgccgaacct gacgctcgag agtacttttg
      421 tccgcgaggg atcgccgatc gcaaacgccc gccgcagggt gcactggtag agactgccct
      481 cctcttcaat ccgcaggaac tgcttgggga actcctcccg ggagagaggc caaagcctgg
      541 ttcccgatcc gcctgcgagc acaagggtga atacgggatt cattcaatca cgtccattcg
      601 tcggtatgtg attctatttt gagagtatag cattccggag atcggaaagt atagaaccgg
      661 ggaggtttcg cagcaagagg acaacaaaaa tcccccctac gcagggggga tttttcagcg
      721 cgtcaaaaag ggagaatcgg gttacttcat cttgacgccg atgccgcgct cctcgtccgt
      781 tcccggaaca agggagtcgc agaaggccgc gcagattccg gcaaccgcca tgggggtctt
      841 cagaatggcc cagaccatgc cgccgagcgt ctgcaacacc tgatggtagg cggggttcat
      901 gaagagctcc tggtttttct ccacccagcc gggaagcccc atcgccatca ggaaggcgaa
      961 accgacgatg aggacgttgc gctggctgcc catgtcggcg cgcatgagca cctggatgcc
     1021 gagggcgccg atggtgccga acagggcgat gtaggcgccg ccgataatcg gggagggcat
     1081 ggtcgcgaca agcgcgccga acttggtcac gaagctcatg agaatcagca ggatggcgcc
     1141 gacgcggacg acccagcggg aggccactcc ggtaaggccg atgaggccga tattctccgt
     1201 gtaggaggtg gtgcccacgg agccgaagag accggagagg gcgcagttca agccctcggc
     1261 gccgataccg cggctgatgg tcttttcatc ggggtcgtca aggccggagg cgtaggagac
     1321 tgagtggtag tcgccgacgg actcgatcat gacggcgaag aagccggcga gcagcgcgcc
     1381 gaacgccaga aggctgaact tgggcgttcc ccacggcatg atgatgtcga tgcggtacca
     1441 gggggccgcc tcgacgttcg caaggctgat atgcgcaggg tgtccctcgg ggaagattcc
     1501 cgctttggaa agtccgaggc agaggaggta ggtgagcacg atcgacgaga ggatcgcgaa
     1561 gatgttggcg tacttgttct tgctcacgag gctgaagaag aagatgagcg ccacgacgag
     1621 cagcgaaacg ggccagtagt tggcggcgtt gaactggatg gccgtaggag caagggaaaa
     1681 gccgatggcc atgatgaccg ggcctatgac cacaggcgtg atgatcttcc ggatgtagcc
     1741 gatgatcccg gtgtagccga ttaccgagag cagaataccg cccgcgatga ggccgccgcc
     1801 gatgtactgc atgatgacct cgggccccga cgcgctgtat gcgccgatga tcgtcatcac
     1861 cgaaggaatg aagctgaagc tcgacccctg gacgatggga agtcccgaac cgagagccgg
     1921 gtgcgtctgg atcagcgtcg cgacgcccat accaaaatag acgcacgaaa tgaagaccgc
     1981 tatctgcgcc tggctcatac ccatagcagg gccgaaaatc agcggaacga gcgttgtggc
     2041 gccgaacaga gtgagcacgt gctgcgctcc ggcaaggatc agaatgggaa acggagggac
     2101 gtcgtgcaac ccgtacacta tctttttcgc catgagagaa tccacctcct gaacaaaatg
     2161 atgtcacaaa aacagtttcg tccggtacag tacctcgcgt gctccatatt ccacctccct
     2221 ctccgaaagg ccgaattccg gaaaggcccc cgaaagctcg ggggccggta tccgctgctg
     2281 tttacttcca ctccttgatg gcgttcttgt acgactgctc cagggcgtag gggtactccc
     2341 aaagatccgt ctctccgttt ttcttcgcct gtatcgcctt gtacacgcgt tcgcgcccca
     2401 gcggataggt gtgaattctg atgcccagcg cgtcgaaaag cgcgttgtcg atcgcggggg
     2461 ccggagagat catcgagtgc tcgccgacgc ctctgttccc gtatggggag agctcttccg
     2521 gcgtctcctc ccagaaggag tccacctcgc gcggcacgtc catgctcgtc gcgatcttgt
     2581 agtcggtgaa gtcgggggtg agcagcttcc cgtgctcgtc gtagtacatc ccctccatca
     2641 gaccggccga aataccctgg atggtgccgc cctcgatctg tcccttcacg ttgatcgggg
     2701 agacggcctt gccgacgtcg tacccggcgg agaccttgtt tacctcgacc gcccctgtcc
     2761 ccggatcgac ctcgatgtcg atgccgaccg cgccgacagt gaagtgtacc acgctcttcg
     2821 gcgactggcc agtctcggga tcggggtaga tcacgtccgg aggcacgaag ctccccgaac
     2881 cgatcacggg gccgccgaca tagttgccgg agggcatcat gaacccgctc tggatctgtt
     2941 cgccgatcgg ctccgccttc cccttcttct tacattttac tataccgtgc tcgatggtga
     3001 tactctccgg ctcggtctcc cagtattcgg agtagagggc gagcagcttc tcgcgcgcgt
     3061 cggtcgccgc gcgtttcacc gccatgccca tcgaccagca tgagcggctg gcgacggtct
     3121 gccagtcgta cgggtggctc tgcgtgtccg gatagtagca cttgatcttc tcgatcggga
     3181 tgccaagctc ttcggaggcc atctgggcgt acgcggtata ggcgccctgc cccatgtcca
     3241 tcgtcgccgc aaggacgtcc acagttccgt cgccaagaat cttcacgatt gcggaggacg
     3301 ccgcgtccga aggcatcgcg ggcgccttca gcgcgagggc gaaccccttg ccgcggat
//
LOCUS       DAOPKU010000327         3349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_31882_length_3349_cov_4.335762, whole genome shotgun sequence.
ACCESSION   DAOPKU010000327 DAOPKU010000000
VERSION     DAOPKU010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31882_length_3349_cov_4.335762"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..289
                     /gene="fliQ"
                     /locus_tag="PK849_10130"
     CDS             20..289
                     /gene="fliQ"
                     /locus_tag="PK849_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HPE66523.1"
                     /translation="MFPVNMNDVLSDAVWLSLTTVLPVLLVAMTVGLVIGILQTATSI
                     QEQTLIFIPKIMAVMIALIFFGPWMFGAIGDFARLIFGQLDRFIK"
     gene            286..1071
                     /locus_tag="PK849_10135"
     CDS             286..1071
                     /locus_tag="PK849_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPE66524.1"
                     /translation="MTAEQTAFVPVLVFYLLTGIRFIGMMFTAPVFSASALPLHLRFW
                     MAFLLTLAAAGSLQTDVPLILFDDWTSIVLLGTRELLIGSLLGFLAALPLYVLQISGE
                     LIGISMGLSMLSVMDPLSQVQVSIIGQLQFLVGMWFYFRWNGHLLMTQAVVESLKLVP
                     PGKLALSISWDLGLGSWLNQAFVLSLRFVLPFYGAILLADVGLGFLARTVPQMNIFVL
                     GLPLKVALGFFVLMVVLPVTVELMAENIERFIEFALMGVTAWR"
     gene            1062..2216
                     /gene="flhB"
                     /locus_tag="PK849_10140"
     CDS             1062..2216
                     /gene="flhB"
                     /locus_tag="PK849_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPE66525.1"
                     /translation="MALEFNLQFFSQERTEPATPRKRRKEREEGRVAKSQDLGAAVVI
                     LTGLFGLLVFGRFMYSYMRDFLVEMIAFMGGSTLREAGWFGVLSRESIPASILPWIPL
                     GLMVAIGGLIVTVAQVGIELTTKPLIPKMDRFNPVSGLKKVISLRSLVELVKGLLKAS
                     LFAAVIYFALQKDTPELVAAIRYPLEIGVSQLFSKLLSLSFRLAFLLLVIAIFDYGYQ
                     KWEFERSIKMSKQELKEEYKQMEGDPQIKSKIRQKQRELARSRMMSSVPKADVVITNP
                     THLAVALEYNREIMEAPVLVAKGSDFLARKIRELAEEHGVPVVENKPLARAIYESMEI
                     GEEIPEHLYKAMAEVLAFIYKLKGAKNRGASPPRTSVSSTEGPPQAPIFM"
     gene            2250..>3349
                     /locus_tag="PK849_10145"
     CDS             2250..>3349
                     /locus_tag="PK849_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HPE66526.1"
                     /translation="MGKVQESTAGMSPFKKMLHYSDIGMAFLVILIVVMMVIPLPTWL
                     LDVMLSANITIGVVVLLSTFYAQRALDLAAFPTMLLIVTLFRLALNVSTTRLILLQAD
                     AGDVVRAFGTFVVGGNYVVGAVVFLILVIIQFVVITKGAERVAEVAARFTLDAMPGKQ
                     MAIDADLNAGLIDDAGAKDRRLTIQREADFYGAMDGASKFVKGDAIAGLIITIINIVG
                     GLAIGVLQRGFPLEKALATYSLLTVGDGLVSQIPALLFSTATGVIVTRAAGQTDLGKD
                     IFTSLTTYPRPLFIGSILLFALAAVPGLPKIPFLLLAAFLCLMGYWVYREGKIQEEAG
                     GKKREGRKEAAPGAPGGEAPKPAPSSPEDVMRL"
BASE COUNT          611 a          908 c          990 g          840 t
ORIGIN      
        1 tgcataagga ggcgatggca tgtttccggt caatatgaac gatgtgttga gcgacgccgt
       61 ctggctctcg ttgacgacag tgttgccggt tcttctcgta gcaatgacgg tcggactggt
      121 gataggcata ctgcagacgg ccacctcgat tcaggaacag acgctcatct tcattccgaa
      181 gataatggcg gtcatgatag cgctcatttt cttcggaccg tggatgttcg gggcgatcgg
      241 agattttgca cgcctcatat tcggtcagct cgaccggttc atcaaatgac tgccgagcag
      301 accgctttcg ttcctgttct ggttttttat ctgctgacgg gaatccgttt catcggtatg
      361 atgtttacgg cccctgtttt ctctgcttcc gccctacccc ttcatctccg tttctggatg
      421 gcgtttctgt tgactctcgc cgcggccggc tcgctgcaga ccgacgttcc gctgatactt
      481 ttcgatgact ggacctcgat tgtcctgctg ggaacccgcg agctgctgat aggttcgctg
      541 ctcggttttc tcgcggcgct tcccctgtac gttctgcaga tttccggaga gcttatcggt
      601 atcagcatgg ggctttccat gttgagcgtc atggatccgc tcagccaggt gcaggtctcg
      661 atcatcggtc agctgcagtt tctggtcggc atgtggtttt atttccgctg gaacgggcat
      721 ctgttgatga cgcaggccgt tgtggagagc ctgaagctcg ttcccccggg caagctcgcg
      781 ctttccatct cgtgggatct ggggctcggc agctggctga atcaggcctt tgtcctcagc
      841 ctgcggtttg tccttccttt ttacggcgcc atcctgctgg ccgatgtggg gctcggcttt
      901 cttgccagaa cggtgccgca gatgaatatt tttgttcttg gcctgcccct gaaagtcgcg
      961 ctcggtttct tcgtccttat ggtggtgctt cccgtcactg tcgagctgat ggcggaaaac
     1021 attgagcgct tcatcgagtt tgcgctcatg ggggtcacgg cgtggcgcta gagtttaacc
     1081 tccagttctt ctcccaggaa cgaaccgaac ctgctacgcc ccgaaagcgc cggaaagagc
     1141 gggaagaggg gcgggtagcc aagagtcagg atctgggggc ggccgttgtg atcctgacgg
     1201 ggctttttgg actcctcgtt ttcggcagat tcatgtattc gtatatgcgg gattttcttg
     1261 tcgagatgat cgcgttcatg ggggggagca cgctgcggga ggccggatgg ttcggcgtgc
     1321 tgtcccgcga gtcgatcccg gcgtcgattc tgccctggat tcctcttggc cttatggtcg
     1381 cgataggggg gcttattgtt accgtggcgc aggtgggaat agagctgacg acgaagccgc
     1441 tgatcccgaa aatggaccgc ttcaatccag tctccgggtt aaaaaaagta atctccctcc
     1501 gttcacttgt ggagcttgtc aaaggcctgc tgaaagcgtc gctctttgct gcagtgattt
     1561 atttcgcatt gcagaaagat actcccgagc ttgtcgccgc gattcgttat ccgctggaaa
     1621 tcggcgtttc gcagctcttt tcgaagcttc tcagcctcag cttccgcctt gcctttcttt
     1681 tgctcgtcat tgcgatattt gactacggct atcagaagtg ggagtttgaa cgctcgatca
     1741 agatgagcaa gcaggagctg aaagaggagt ataagcagat ggaaggagat ccccagatca
     1801 agagcaagat ccgccaaaag cagcgcgagc tggcacgcag caggatgatg tcctccgttc
     1861 ccaaggcgga cgttgtcatt accaacccga cgcacctggc cgtggcgctt gaatacaacc
     1921 gcgagattat ggaggcgcct gttctggttg ccaagggaag cgattttctt gcgaggaaga
     1981 tacgtgagct cgccgaggaa cacggcgtcc ctgtggtgga gaataagccg cttgccaggg
     2041 ctatctacga atcaatggag atcggagagg aaatccccga acacctgtac aaggctatgg
     2101 ccgaggtgct ggccttcatt tacaagttga agggagcaaa aaatagaggc gcttctccgc
     2161 cgcggacttc tgtttcttcg accgagggtc cgccccaagc gccgattttc atgtaaaata
     2221 ggaaacagcc aaggaagcga tacggaagga tgggaaaggt gcaggaatcg actgcgggga
     2281 tgtctccgtt caaaaaaatg cttcactact cggatatagg tatggccttc ctcgtgatcc
     2341 tcatcgttgt gatgatggtc attccgctgc cgacgtggct tctggacgtg atgctttcgg
     2401 ccaatatcac tatcggcgtg gtggtgctgc tgtcgacgtt ctacgcgcag cgtgcgctcg
     2461 acctcgcggc ctttcctacg atgctgctga tcgttacgct cttccggctt gcactgaacg
     2521 tctctacgac gcgtctcatc ctcctgcagg cggatgccgg cgatgtcgtc agggcgttcg
     2581 gtacgtttgt cgtcgggggg aactacgtcg tgggcgcggt agtcttcctg atactcgtca
     2641 ttatccagtt cgtcgtcatc accaagggcg ccgaacgcgt ggcggaggtt gccgcgcgct
     2701 ttacgctcga cgccatgccc ggtaagcaga tggccatcga tgcggacttg aacgccggtc
     2761 tgatcgacga cgcgggcgcc aaggaccggc gcctcactat tcaacgcgaa gcggattttt
     2821 acggcgcgat ggacggcgcg tccaagttcg ttaagggcga cgccatcgcc ggtttgatca
     2881 tcacgatcat caacatcgtg ggcggtctcg ccataggtgt tttgcagcgt gggtttcctc
     2941 tggagaaggc gcttgcgacc tacagtcttc tgaccgtggg cgacggtctt gtttcgcaga
     3001 tcccggcgct gctcttctcg acggctaccg gcgtcatagt cacccgcgcg gcagggcaga
     3061 cggaccttgg caaggatatc tttacgtcgc tcacgaccta tcccagacct cttttcatag
     3121 gttccatcct ccttttcgca cttgctgcgg tccccggtct tccaaagatt ccctttctgc
     3181 ttttggcagc ctttctctgc ctgatggggt actgggtcta ccgcgaggga aagatacagg
     3241 aggaagcggg cggcaagaaa agggaaggtc gaaaggaagc cgctccgggc gctcccgggg
     3301 gcgaggcccc gaaaccggct ccctcctctc ctgaagatgt gatgcgtct
//
LOCUS       DAOPKU010000328         3346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_31933_length_3346_cov_3.457612, whole genome shotgun sequence.
ACCESSION   DAOPKU010000328 DAOPKU010000000
VERSION     DAOPKU010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31933_length_3346_cov_3.457612"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PK849_10150"
     CDS             complement(<1..1212)
                     /locus_tag="PK849_10150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE66527.1"
                     /translation="MRLGEILLQEGIVDVQTLERALSIQQKSGAQIGDVLIALGKINP
                     LQLAKALSLQFNLPYRNAVDYPANPETLKEVVLVVGEQFLRTNAILPLGRKGNALGVV
                     TSRPNAKEPLEALAKAMNAELFLFVCSEREIQTALTNHFRDSYTEESTMGLFFRNPEE
                     SAMETFTTLQLVLLYSLVTLFLFGMLFRGRETLLVLFFLINVFYVFSLGFKLLASILG
                     ARTEILEQVSDEEAAALDPKTLPVYTILLPMYKEPEVVKHLIAGIMEMDYPLEKLDVK
                     ILLEEEDTVTYDAVRALRPPANFEIVRVPYSVPKTKPKACNYGLLFARGEYLTIYDAE
                     DVPEKDQLRKVVAAFRKGPENMICVQAALNYFNSDQNLLTRMFTLEYSFWFDYMLPGI
                     EALGMPIPLGGT"
     gene            complement(1219..2049)
                     /locus_tag="PK849_10155"
     CDS             complement(1219..2049)
                     /locus_tag="PK849_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66528.1"
                     /translation="MKTAARRAILTVCVFCVACFSSASPLFAAAWVQPYGETFFSFSM
                     SDYESGWYRDEEGTRRKSGDGAYRKREWKLYIEHGLTEETTLLFALPWQRAEKLAPSN
                     FSESGFHDLEIGIRRRLWARGFETASWQALVGIPAGYDTEKAFRLGEGNAYLDLRYLW
                     GRSYEMSGRYGYISLEGGIRHFLEGVEVDQFRMDGTWFHPFSKKWGLQSTFSVIEGFS
                     ISSAPGRNGFSLWKAGAGVVYSPTDQTDIMVRNIWDFAGKNTGDGSNLELVFSTRIGR
                     "
     gene            complement(2052..>3346)
                     /locus_tag="PK849_10160"
     CDS             complement(2052..>3346)
                     /locus_tag="PK849_10160"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HPE66529.1"
                     /translation="GRSASSSFILIAGDAIQLPSGIQPLPGLSLLGERGLLVLGDGKP
                     ASLAGFVKTIASFPLFAAIPSGSILPETSAASEKNAVSNLLNLAEGTGANAVSLEIPV
                     FPGLLASPPQDLSFQLEGACTLPRDPARPARLDVFWNGVFIESEYLSANGRFEKRLLL
                     PVETPLLTENRLRLDVSYHLDEGMCRYRDSENRVTLFPSSSFHGYGKYPLHALSWNSF
                     GIFALRKGILLLEEPFSIEGIRSAADMLHWLGGRYPRDVFIFPELRTTSEPSSYLKDA
                     EWILAATSTEAIPRDLQQLLPLNVHEGFLLEIQRDGKTTYSFNREDDVAVAVIGSSSK
                     PVLLLASSSPKMMSEAAAFFASPGSAARLSGNLMSFRSQEDVRVFDTRAPHVGVKRGA
                     VLSFAERIWLRYRGFFIIGLWILATVLFATLFLKKRKV"
BASE COUNT          771 a          980 c          967 g          628 t
ORIGIN      
        1 cgtgccgccg agcggaatgg gcatccccag cgcctcgatt ccgggcagca tatagtcgaa
       61 ccagaacgag tactccagcg tgaacatcct cgtcaacaga ttctggtcgg agttgaagta
      121 gttcagcgcc gcctgaacgc atatcatgtt ctcaggccct ttacggaagg ccgcgacgac
      181 cttccggagc tggtccttct cgggaacgtc ttcggcgtcg tagatggtga gatactcgcc
      241 gcgcgcaaag agcaggccgt agttgcaggc cttcggtttt gtcttgggaa cggagtaggg
      301 cacgcggaca atttcgaaat tcgcaggcgg acgcagagcg cggacagcgt cgtaggtgac
      361 cgtatcctcc tcttcgagaa gaatcttcac gtcgagcttc tccagagggt agtccatctc
      421 catgattccg gcgatcagat gtttgaccac ttcgggctct ttatacatcg gaagcagaat
      481 cgtatagacc ggcagcgtct tcggatcgag cgcagcggcc tcctcatcgc tcacctgttc
      541 caggatctcg gttcgcgcgc ctaagatgga ggcgagcagc ttgaatccca gcgaaaagac
      601 atagaagacg ttgatgagga aaaagagcac gagcaacgtc tcgcgcccgc ggaaaagcat
      661 gccgaaaagg aagagtgtaa ccagggagta caacaggaca agctggagcg tagtgaaggt
      721 ctccatggcg gactcctccg gattccggaa gaagagcccc atagtgctct cttcggtgta
      781 cgagtcacgg aagtggttcg tcagcgccgt ctgtatttcc cgctcgctgc agacaaagag
      841 aaagagctcg gcgttcatag ccttcgcgag cgcctcaaga ggctccttcg cgttggggcg
      901 cgaggttacg acgccaagag cattcccttt tcttcccaag ggcagaatgg cgttcgtccg
      961 aaggaattgc tcccccacta ccaggacaac ctctttgagc gtttcggggt tcgccggata
     1021 gtccacggcg ttccggtagg ggaggttgaa ctgcagcgaa agcgccttcg ccaactgaag
     1081 agggttgatt ttgccgaggg cgatcagcac atctcctatc tgcgcgccgc tcttttgctg
     1141 tatcgagagc gcgcgttcca aagtctgcac atcgacgatc ccttcctgaa gtaaaatttc
     1201 acccaagcgc acgacgtgct atctcccgat acgcgtactg aagacgagtt ccaggttcga
     1261 gccgtctccg gtgttctttc ccgcgaaatc ccagatattc cgcaccatta tgtcggtctg
     1321 gtcggtgggg gaatagacaa cgcccgcccc ggccttccag agcgagaatc cgttccgccc
     1381 cggagcgctg ctgatggaaa acccctcgat gacgctgaac gtcgattgta ggccccattt
     1441 cttggagaac gggtggaacc atgtgccgtc catacggaac tggtccacct cgacgccttc
     1501 gagaaagtgc cggattccgc cttcaaggct gatatagccg tagcggccgc tcatctcgta
     1561 ggatctgccc caaagatacc gaagatccag atacgcgttc ccctcgccga gacggaacgc
     1621 cttctccgtg tcataccctg ccggaatacc gacaagcgcc tgccatgaag cggtctcgaa
     1681 cccccgggcc caaagtctcc ggcgtattcc gatctccaga tcgtggaagc cgctctccga
     1741 aaagttcgaa ggcgcaagct tctccgcccg ctgccacgga agggcgaaga gaagcgtcgt
     1801 ctcctcagtc aggccgtgtt caatgtacag cttccattcg cgcttcctgt aggcgccgtc
     1861 cccgcttttg cgccgcgtgc cttcctcgtc ccgataccag ccgctctcgt aatccgacat
     1921 ggaaaaagag aaaaacgtct cgccgtacgg ctgaacccag gctgcggcga aaagagggga
     1981 cgcggaggag aagcacgcaa cacaaaaaac acacaccgtc agaatcgcgc gccgtgcagc
     2041 cgttttcatg atcacacctt cctcttcttc aaaaagagcg tcgcaaacag caccgtcgcc
     2101 agaatccaca agccgatgat gaaaaagcct ctgtaccgca gccagatgcg ctccgcgaac
     2161 gaaagcactg cgccacgttt aaccccgaca tgcggagcgc gagtatcgaa aacgcgcaca
     2221 tcctcctgtg aacggaagga catcagattg ccggagagac gcgcggcgct tcccggagac
     2281 gcgaagaacg cggcggcttc gctcatcatc ttcggcgacg aggaagcgag cagaaggacg
     2341 ggcttcgagg agctgcctat cactgcgacc gcgacatcat cctctctgtt gaacgagtac
     2401 gtcgtcttgc cgtcccgttg aatctcgagc aaaaaccctt catggacatt caacggaaga
     2461 agctgctgaa gatccctggg gatagcctct gttgacgtcg ccgccagaat ccattctgcg
     2521 tccttcagat acgaagaagg ctccgacgtc gtgcgaagct ccggaaaaat gaagacgtcg
     2581 cgggggtatc gtccgccgag ccaatggagc atatccgcgg cgctgcgaat accctcgatg
     2641 gagaacggtt cttccagaag cagaatccct ttgcggagcg caaaaatacc gaaggagttc
     2701 cacgacagag cgtgcagagg gtacttcccg tagccgtgga aggaggagga aggaaacagc
     2761 gtcacgcggt tttccgagtc ccggtagcgg cacattccct catcgaggtg atacgatacg
     2821 tcgagacgaa gacggttctc cgtcaacagc ggcgtttcga ccggaaggag caggcgtttt
     2881 tcgaatcttc cgtttgcgga caggtactcg ctctcgatga acacgccgtt ccagaaaacg
     2941 tcaagacgcg ccggacgggc aggatcccgc ggcagcgtac acgccccttc gagttggaag
     3001 gagagatcct gcggaggcga agcgaggagc cccgggaaaa cggggatttc aagagagacg
     3061 gcgttggcgc ccgttccctc cgccaggttc agcagatttg aaacggcatt tttttcggaa
     3121 gccgcgctcg tctcgggcaa aatacttccg gaagggatcg ccgcgaagag cgggaaagaa
     3181 gcgatcgtct tcacgaagcc ggcgagcgaa gcaggcttgc cgtcgccaag aacaaggagt
     3241 cctctctcgc cgagcagcga tagacccggc aggggctgaa ttccggaagg aagctgaatg
     3301 gcgtcacccg cgatcaggat aaacgaagag gatgcagagc ggccgt
//
LOCUS       DAOPKU010000329         3330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_32142_length_3330_cov_3.382595, whole genome shotgun sequence.
ACCESSION   DAOPKU010000329 DAOPKU010000000
VERSION     DAOPKU010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32142_length_3330_cov_3.382595"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PK849_10165"
     CDS             complement(<1..893)
                     /locus_tag="PK849_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE66530.1"
                     /translation="MDEFGVRNLRQAVASVGRDDLIQLCQELLRRPGVSGREGDVARF
                     VAESMRSLGFDSVVIDSYGNVVGEIIFSAEGPRLLLTAQMDHVDAGDVAEWSKYPFGA
                     FIEENRIYGRAASDQKGALASMMLAASLLKRDRSGVLQGRLFVAGAVHQETFERVASK
                     AIGDFADPDCVIVGEASGLLLERGQRGRAEIRVDIYGRMAHSSHPEFGVNAADVMVEF
                     LSFLKSRFTPPRDEFLGEGILVLTNLFSSPSYNRESSQREARPDSSAIPERCTAVFDR
                     RLLLGETLEGVVRQFEEIVNEA"
     gene            1025..1933
                     /locus_tag="PK849_10170"
     CDS             1025..1933
                     /locus_tag="PK849_10170"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPE66531.1"
                     /translation="MNLHQLRIFCTIVEEGSFRQAAEKLFLSQPSVSQQVAALEKSYD
                     VRLFERKGRSVSLTPEGRALHVLASDLLRQADEIPARFRDMRALRSGRLEIGVSPFAG
                     FYILPKALVGFRENFPAVSISVTSGNTTEILRDLKRGNVEMVILGKSFPFSRDPALTY
                     RSLGEDRLVLAAPQGHPWASTGQASFTDAERRTLIRFAGDCPLATYVDEFLLRNRIVF
                     GEQVETDEIELAKNLAEAGVGVLITSSLSVQRSIASGRLAPVMLEGMDDMSWEIQCVY
                     SSSKGLSYPGWEMAKRLETTCRELLH"
     gene            complement(1984..>3330)
                     /gene="pepF"
                     /locus_tag="PK849_10175"
     CDS             complement(1984..>3330)
                     /gene="pepF"
                     /locus_tag="PK849_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPE66532.1"
                     /translation="SPREEELLARSSEMARTAETAFSLFTNADMTFPSIVDEKGEETE
                     LSEERYYLLSRSRDRRVRKEAFEGLLGTYGKYRNSLGALYSGSVKGDIFYATARKYQS
                     SLDASLDADNIPSSVYNNVVSTVNANLDAMHDYMCLRKKALALDELHMYDLNVPLADE
                     PDAAIPYGKAVEMVTEGLAPLGEEYIEVLRNGFTTRWIDVYENQGKHKGAYSWGSYGA
                     HPFVLLNYSGTLRDVFTIAHEMGHSLHTWHSQERQPQVYADYTIFLAEVASTVNESLL
                     LESLLEKASREEKIYLLNHYLDQVRTTVYRQAMFAEFERDMHAAAEQGAPLTADSLCN
                     AWRELNERYHGPAMTVDADIEVEWARIPHFYSAFYVYKYVTGFTAAAMLTRQLRKGSA
                     EERERYLRFLSRGSSAYSLDILKEAGVDMTTSTPLEETVANFREKTKQLSGLLGID"
BASE COUNT          680 a         1070 c          918 g          662 t
ORIGIN      
        1 gcctcgttca cgatctcctc gaactgcctg acgacgccct ccagcgtctc cccgagcagc
       61 agccgcctgt cgaaaacagc tgtgcatcgc tcgggaatcg cgctgctgtc gggacgggcc
      121 tcccgttgag aactctctct gttgtatgac ggtgaggaaa aaagattggt gaggacaaga
      181 attccctcgc cgaggaattc gtcgcgcggc ggggtgaaac ggctcttgag aaaggaaaga
      241 aactcgacca taacgtccgc cgcgttcacc ccgaactcgg gatgcgacga gtgggccatt
      301 ctgccataga tatcgacgcg tatttccgcc ctccccctct gcccccgttc gagcaagagg
      361 ccggaggcct cgccgacgat cacgcagtcc gggtcggcaa aatctccaat agccttcgaa
      421 gcgacccgtt caaacgtctc ctggtgcacc gctccggcga caaagagcct gccctgcagc
      481 acgccgctcc ggtctcgttt cagcagagac gcggcaagca tcatcgaggc aagcgcgccc
      541 ttctggtcgg aggcggctct tccataaatg cgattctcct cgatgaacgc gccgaagggg
      601 tacttggacc actcggcaac gtctcccgca tccacatgat ccatctgcgc cgtgaggaga
      661 agcctcggcc cctccgcgga gaatatgatc tctcccacga cgttgccgta tgaatcgatc
      721 acgacggagt caaacccgag ggaacgcatg ctctcggcga cgaaacgggc gacgtcgccc
      781 tcacggcccg acacgccggg tctgcgaagg agctcctggc aaagctgaat cagatcgtcc
      841 cgcccgacgg aagcaacagc ctgccgcagg ttccgaacac cgaactcatc catgccatcg
      901 cctccttgct ctccgtgtac gctctctacc ttctcttgct accaagatac accagatagt
      961 attatttgca agaaggaaaa aatgatataa tacttcagga aaacctatat caaggaggca
     1021 acggatgaat cttcaccagc tgaggatatt ctgcacaatc gtggaagagg gctccttccg
     1081 ccaggctgcg gagaagctct tcctgtcgca gccctccgta agccagcagg tcgccgcgct
     1141 cgaaaaaagc tacgacgtcc gtctcttcga acgaaaggga cgctcagttt cgctgacccc
     1201 cgaaggacgc gcgctccacg tgctcgcctc ggacctcctc cgtcaggcgg acgagattcc
     1261 cgcccggttc cgcgatatgc gggccctccg ttcagggcgg ctggaaatag gcgtttcgcc
     1321 gttcgcaggc ttctatattc ttccgaaagc gctcgtcggc ttccgggaaa acttccccgc
     1381 cgtctccatc tccgtcacat cggggaatac caccgagatt ctgcgggatc tgaaacgggg
     1441 aaacgtcgag atggtcatcc tcggaaagag cttccctttt tcaagggatc cggcgctcac
     1501 ataccgttct ctcggcgagg acagactcgt tctggccgcc cctcaaggac acccctgggc
     1561 ttcgaccggt caggcgtcgt tcaccgacgc ggagcgccgc accctgatcc ggttcgcggg
     1621 cgactgcccc ctggctacct acgttgacga atttttgctc cgcaacagga ttgtcttcgg
     1681 ggaacaggtt gaaaccgacg aaatcgagtt ggcgaagaat ctggccgagg ccggcgtcgg
     1741 ggtcctcatc accagctcac tctcggtgca gagaagcatt gcttcaggcc gcctcgcgcc
     1801 cgtcatgctc gaaggcatgg acgacatgtc gtgggagatc cagtgcgtct actcatcctc
     1861 gaaaggcctc tcctaccccg gctgggagat ggcaaaacgc ctggaaacaa cctgccgcga
     1921 actgctgcac tgaagaacgc cactctatgc aagaggggct ccttcggagc ccctcttttg
     1981 ttctcaatcg atccccagaa gcccggagag ctgctttgtc ttttccctga agttcgccac
     2041 ggtctcctca agcggtgtgg aggtggtcat gtccacacct gcctccttca gaatatccag
     2101 agaatacgcg gaactccctc tcgaaaggaa acgcaggtag cgctcgcgct cttcggcgct
     2161 tcccttccgg agttggcgcg tgagcatcgc cgcagcggta aaaccggtta cgtacttgta
     2221 gacgtagaac gcggagtaga aatgagggat acgcgcccac tccacctcga tgtccgcgtc
     2281 gaccgtcatg gcgggcccgt gatagcgctc gttcagctcc cgccatgcat tgcaaagaga
     2341 atccgccgtc aggggcgctc cctgttccgc tgcggcgtgc atatcccgct cgaactccgc
     2401 gaacatcgcc tgacgataga cggtagtgcg cacctgatca aggtagtgat tcagaagata
     2461 gattttttcc tcccgcgagg ccttctccag caacgattcc agcagcagcg actcgtttac
     2521 cgtggaggcc acctccgcga ggaagatcgt gtagtcggcg tacacctggg gctgcctctc
     2581 ctgcgagtgc cacgtatgca gcgagtgccc catctcatgg gcgatggtga agacgtcgcg
     2641 gagcgtgccg ctgtagttca gcagcacgaa cgggtgcgcc ccgtagcttc cccacgaata
     2701 cgcgccctta tgtttcccct ggttctcata cacatcaatc caccgggtgg tgaatccgtt
     2761 gcggagtacc tcaatatatt cctctccgag aggggcaagc ccctcggtca ccatctccac
     2821 cgccttcccg taaggaatgg ccgcgtcggg ttcgtccgca agcggtacgt tgaggtcgta
     2881 catgtgcagc tcgtccaggg cgagcgcctt cttcctgaga cacatgtagt cgtgcatcgc
     2941 atccaggttg gcgttcaccg tcgagacgac gttgttgtag accgaggagg ggatgttatc
     3001 cgcgtcgagc gaagcatcca gcgaagactg gtacttccgc gccgtcgcat agaagatatc
     3061 ccccttcaca ctcccggaat agagcgcgcc gagcgagttc cggtacttcc cgtaggtccc
     3121 cagcagacct tcgaacgcct ctttgcgcac gcggcgatcc cgcgaacggc tcagaaggta
     3181 gtaccgctcc tctgaaagct ctgtttcctc ccccttctcg tcgacgatcg acgggaaggt
     3241 catatcggcg ttcgtaaaaa gcgaaaacgc tgtctccgcc gtacgggcca tttcggaaga
     3301 acgcgccaga agttcctctt cgcgcggcga
//
LOCUS       DAOPKU010000330         3313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_32342_length_3313_cov_3.396869, whole genome shotgun sequence.
ACCESSION   DAOPKU010000330 DAOPKU010000000
VERSION     DAOPKU010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32342_length_3313_cov_3.396869"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK849_10180"
     CDS             complement(<1..634)
                     /locus_tag="PK849_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HPE66533.1"
                     /translation="MTHSRSNNFQHTRLWCEGGLFCVEATNGGAGAFVLKAETLGGLG
                     LGSAITVVVGSWIAGAVMGVDLFRFNRSAGAVLAGAAACFIFTNPLLNIVGYIGAVSV
                     GQFNYVEWMLEKNMLLALVGVLAWTTSLWTTNNAELYCNALYTGPVFWSYGKRIERKT
                     LVLWAGVLGTLLGSLAFYQIFFADFITVLGAAAPPLAGPLIADYYFVKKGR"
     gene            complement(655..1563)
                     /locus_tag="PK849_10185"
     CDS             complement(655..1563)
                     /locus_tag="PK849_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HPE66534.1"
                     /translation="MKKRVLCTTVVLLFLLLFANAASAKTALVFQTDFGLKDGAVSAM
                     KGVAFGVDAGLPMFDLTHEISPYSIWEAAYRLHQTMEFWPAGTVFVSVVDPGVGTERA
                     SVVALTKDGRYIVTPDNGTLTFIVETPGIVAVRTIDDARHRLAGSEQSYTFFGRDLYA
                     YTGAKLAAGAITFEEVGPLFEGEIVRIPYRKAEVKEGAVFGNIPVLDIQYGNVWSSIG
                     KALFDELKPRIGANFDVAIFRGEEKVFGGVIPYVNTFGDVPEGKPLLYFNSLMNLSLA
                     LNMDSFAETHGIESGPEWSLSVRPAE"
     gene            complement(1593..2264)
                     /locus_tag="PK849_10190"
     CDS             complement(1593..2264)
                     /locus_tag="PK849_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66535.1"
                     /translation="MHFEILVEDQSGRKLLEILVPKIIGREHTFRVSAYKGIGRLPKG
                     LRSGEEARRRILLDQLPRLLKGYGRTFAYYPDDTVAVVVVCDLDDKCLKHFRRELLDL
                     LGRCEFKPKTRFCIAVEEGEAWLLGDLSAVKKAYPRAREPILKAYENDSICGTWERLA
                     DAVYPGGSRALSAAGWQVVGAEKSLWAEKIAPHMNVAENLSPSFCYFCRKIRELLEEC
                     SGALG"
     gene            complement(2269..>3313)
                     /locus_tag="PK849_10195"
     CDS             complement(2269..>3313)
                     /locus_tag="PK849_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE66536.1"
                     /translation="CDGPIEFEVYYKEDGNARPITYELSIGPDESGRPCVLMERLRQR
                     RKGQKHGWPFSFLILNDGKGIVWKGEKEGRQIDESQKNLDLSGLLRAIQSDDAGEESK
                     ETEVVELEDRRRLGIATLGSLKQHPRISAFRRFIEGWYLSYFRPDSARALPLAGPQMH
                     LNVHGDNIGNVVQYMERDQPARFRAVLDRIADRIPGIRKIDTERTSDGRLLLRFNDEG
                     FQDPFYAQQMSDGTLKLFAYMLLLADPTPPPFLCIEEPENGLYHKLLEILANEFRDHA
                     TGRKGSSQVFITTHQPYFVDALLPEEVWILEKRADGFSSIRRASDDALVRNMVEEGLP
                     LGSLWYSDYLDAR"
BASE COUNT          733 a         1053 c          816 g          711 t
ORIGIN      
        1 accgcccctt tttgacgaag tagtagtccg cgatgagcgg cccggccagc ggaggggctg
       61 ccgcccccag cacggtgatg aagtcggcga agaaaatctg gtagaacgcc agcgaaccga
      121 gcagcgtccc cagaacaccc gcccacagca caagcgtttt tctttcgatc cgcttgccgt
      181 acgaccagaa gaccggcccc gtgtacagcg cgttgcagta caactccgcg ttgttcgtgg
      241 tccacagcga ggtcgtccac gcgagaacgc cgaccagcgc cagcagcatg ttcttctcca
      301 gcatccattc gacgtagttg aactgcccta ccgacaccgc gccgatgtac ccgacgatgt
      361 tgagcagggg gttcgtgaag atgaagcagg ccgcggcgcc tgcaaggaca gcccccgcgc
      421 tcctgttgaa gcggaagaga tccacgccca tcacagcccc cgcgatccag ctgcccacga
      481 ccaccgtgat cgcgctcccc agtcccaggc ctccgagagt ctccgccttc aaaacgaacg
      541 ccccggcccc gccgtttgtc gcttcgacac agaagagccc tccttcgcac cataaccggg
      601 tatgttgaaa gttgttcgag cgagagtgcg tcacaaacac tactccccct gcggctactc
      661 cgcgggacgg acggaaagcg accactcggg ccccgattct attccgtgcg tttctgcgaa
      721 gctgtccata ttcaatgcca gcgagaggtt catcaggctg ttgaagtaga gcaagggctt
      781 cccctccggc acgtcgccga aggtgttgac gtacggaata acgcctccga agaccttttc
      841 ctctccccga aagatcgcga cgtcgaaatt tgctccgatc cggggcttca gctcgtcgaa
      901 gagcgccttg ccgatgctgc tccagacgtt gccgtactgg atgtcgagca cgggaatatt
      961 gccgaagact gcgccctcct tcacctcggc cttcctgtac ggaatccgga cgatctctcc
     1021 ctcgaagagc gggccgacct cctcgaacgt gatcgctccc gcggccagct tcgcccctgt
     1081 gtacgcgtac aggtcgcgtc cgaagaaggt gtacgactgt tccgaccccg caagacggtg
     1141 ccttgcgtcg tcgatcgtcc gcactgccac gatgccgggc gtctccacga tgaaggtcag
     1201 cgtgccgttg tccggcgtga ctatgtatct tccgtccttc gtcagggcga ccacggaagc
     1261 ccgctccgta cctacgcccg gatcgacaac cgacacgaag accgttccgg cgggccagaa
     1321 ctccatcgtc tgatgcagtc tgtaggcggc ctcccagatc gagtacggag aaatctcgtg
     1381 cgtcaggtca aacatcggca agccggcatc cacgccgaac gccactccct tcatcgcgga
     1441 aaccgccccg tccttcagtc cgaaatccgt ctgaaacaca agcgcggttt tcgcagaggc
     1501 cgcattcgcg aagagcagca gaaagagcaa aactacagta gtgcacagca ctcttttttt
     1561 catcccacat tccacctttt cttcttcaca attcacccaa gcgccccgga acactcttca
     1621 agcaactcgc gaatctttct gcaaaagtag caaaaacttg gcgagagatt ctcggctaca
     1681 ttcatgtgcg gagcgatttt ttcggcccac aacgacttct ccgctcctac gacctgccat
     1741 cctgctgccg acaaagctcg cgagccgcct ggatagacgg cgtcggcaag acgctcccat
     1801 gtaccgcaaa ttgaatcgtt ctcatacgct ttcaatatgg gttcgcgcgc cctcggatac
     1861 gcttttttca ctgcggaaag atcgccgagc agccacgcct ctccctcttc caccgctatg
     1921 cagaatcgag tcttcggttt gaactcgcac ctaccaagaa gatccaaaag ttctctgcga
     1981 aaatgtttaa ggcacttatc gtcaagatcg cacactacaa caactgctac cgtatcgtcc
     2041 gggtaataag caaatgttct tccataccct tttaaaagcc gtggtagctg gtcgagaaga
     2101 attctcctgc gtgcttcctc tcctgatcga aggcctttgg gaagacgtcc tattcccttg
     2161 taggcgctca ctctaaaggt atgctcccgt cctataatct tcggcacgag gatttccaat
     2221 aacttcctcc ctgattggtc ctcgacaagt atctcgaagt gcacggcgct acctcgcgtc
     2281 cagatagtcg ctgtaccaaa gagaacccaa gggaagccct tcctcgacca tattccgtac
     2341 caaagcgtcg tcgctcgccc gcctgattga agaaaaaccg tccgccctct tctccagaat
     2401 ccacacttcc tcagggagaa gcgcatccac gaagtagggc tgatgggttg tgataaaaac
     2461 ctgcgaactt cccttacgcc ctgtggcatg atcccggaac tcgttcgcga gaatctcgag
     2521 cagtttgtga tagagcccgt tctccggttc ctctatacag aggaacggag gaggcgtagg
     2581 gtccgcaagc aaaagcatat aggcgaaaag tttcagcgtg ccgtcggaca tctgctgcgc
     2641 ataaaaaggg tcttgaaagc cttcgtcgtt aaacctgagc agtagacgtc cgtcgctcgt
     2701 tctctcggta tctattttac gaataccggg gattctgtct gcaattctgt ccaaaactgc
     2761 ccggaaacga gccggttgat cccgctccat atactgcacc acgtttccta tgttatcccc
     2821 atgtacattc aagtgcatct gcggaccggc gagcggaaga gccctggcgg aatcgggccg
     2881 gaaatagctc agataccacc cttcgataaa gcgacggaag gctgaaatcc tgggatgttg
     2941 cttcagagaa cccaaagttg ctataccaag acgtcttctg tcttccagtt ctacaacctc
     3001 ggtctccttc gactcctctc ctgcatcatc cgattgaatg gcacgcagca acccagagag
     3061 atccagattc ttttgggact cgtcgatctg gcgcccttct ttttcccctt tccagacaat
     3121 tcctttcccg tcgttcagta taagaaacga aaaaggccaa ccgtgctttt gtcccttgcg
     3181 cctctggcga agacgctcca tgaggacaca gggacgaccg ctctcgtccg gacctatcga
     3241 caactcgtaa gtaatagggc gcgcattacc gtcttctttg tagtagactt cgaactcgat
     3301 gggaccgtcg cac
//
LOCUS       DAOPKU010000331         3298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_32551_length_3298_cov_3.813753, whole genome shotgun sequence.
ACCESSION   DAOPKU010000331 DAOPKU010000000
VERSION     DAOPKU010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32551_length_3298_cov_3.813753"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3298
                     /locus_tag="PK849_10200"
     CDS             <1..>3298
                     /locus_tag="PK849_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPE66537.1"
                     /translation="GPHLKIYQCGLPKKMALELFKPFVIRQLVERNLAPNVKSAKRII
                     ERGREEIWGILEEIVKDHPVMLNRAPTLHRLGIQAFEPVLMEGKAIRLHPMVCTAFNA
                     DFDGDQMAVHVPLSIEAQTEARLLMLSANNLLSPASGRPVMTPTQDIVLGIYYLTDMR
                     DGLPGDGMYFSDIDDALAAFHHGVTKVNSRIFLRRRDEWNHALGSVEGKWIETSPGRV
                     IFNSRLPEPLRFINRQLGKKDLGQLLDRAFDLVDRVSMVTMLDNIKSLGYHWATVSGI
                     SFGVQSIIIPPEKRELTAKAMEQDEELKSEYNMGILTPDEYLFQKENLWSETSRAIAE
                     SIVDHMDRTNPIRMMSDSGARGSRSQMGQMAGIRGLMSDPSGRIIDYPITANFREGMN
                     MLEYFISTHGARKGLADTALRTAKSGYLTRRLVDVAQDLIITQHDCGTNQGVLIRPLT
                     QDGKVMIPLSERIYGRTSLHDIEHPETGGMIVASGECITDEKARIIEKSGIEEVWVRS
                     PLSCALRHGICQVCYGMDLATRKEVPMGEAVGVVAAQSIGEPGTQLTMRTFHTGGVRL
                     TGEDITQGLPRIEQLFEVRRPKKVAFLAGVDGRVTEVRVMEGKRKVIITAENPTGDGE
                     EKVTYNIPINQNLLVNEGDSVLKSDKLTEGNFDPQQLLEVEGIEAVQRALVDEIQEVY
                     RSQGVSISNKHIEVILRKVAPVNRVKINEEGDTSFVAGDLVWTDDIDAETAEIGRENE
                     RNIDEAVRIFTGIPLKDVSAQKMNEAVLQHLGKPLDEEAIRTILRPGMLISSAILTDT
                     EGKQFNLVVGEAAFRKQLEGLELIEAFQSADGRRIEPGTRLTLGQLAIVTSNDPTPLL
                     VRDEALLEKLVDTAYIAEDIVADGESVAKADRVFTLEDAALCRERGVKSVMVWHSVER
                     VNLLDILEESLKEKWGKSLTRAIDIEGNAIADLPQLVDERVIKGILEGRIGAVEIEGA
                     VFSRDHFMKELLSARVYGKILLEPVVDARGNVLAEAAQEVNQTVIERLSGSAGPTDAS
                     GNGVKELVVRPMGGHKKEEKKIIQRVTFVRKLREGPKVKPFVHGITKAALATDSFLSA
                     ASFQQTAQVLA"
BASE COUNT          727 a         1004 c         1046 g          521 t
ORIGIN      
        1 cggcccgcac ctcaagattt accagtgcgg cctgccgaag aagatggcgc tcgaactctt
       61 caagccgttc gtcatcaggc agctcgtcga gcgcaacctc gcccccaacg tcaagagcgc
      121 gaagcgtatc atcgaacggg gacgggagga gatctgggga atcctcgagg agatcgtcaa
      181 ggaccacccg gtcatgctca accgcgcgcc gacgctccac cgtctcggca tccaggcgtt
      241 cgagcccgtg ctcatggaag gaaaggcgat ccgccttcac cccatggtct gcacggcctt
      301 caacgccgac ttcgacggcg accagatggc cgtccacgtg ccgctctcca tcgaggcgca
      361 gaccgaggcg agactcctca tgctctcggc caacaacctg ctgtcgcccg cgagcggccg
      421 ccccgtcatg acgccgacgc aggacatcgt gctcggcatc tactacctga cggatatgcg
      481 cgacgggctt cccggcgacg gcatgtactt cagcgacatc gacgacgcgc tcgccgcctt
      541 ccatcacggc gtcaccaaag tgaacagccg tatcttcctg cggcggcgcg acgagtggaa
      601 ccacgccctt ggaagtgtgg agggcaaatg gatcgaaacc tctccggggc gcgtcatctt
      661 caacagccgg ctccccgagc cgctgcgctt catcaacagg cagctcggca agaaggacct
      721 cggacagctg ctcgacaggg cgttcgacct cgtcgacaga gtttccatgg tgacgatgct
      781 cgacaacatc aagtcgctgg ggtaccactg ggcgacggtc agcggcatca gcttcggcgt
      841 gcagtcgatc atcatcccgc cggaaaagcg cgagctcacg gcgaaggcca tggagcagga
      901 cgaagagctg aagtcggagt acaacatggg tattctcacc ccggacgagt acctcttcca
      961 gaaggagaac ctgtggtcgg agacgtcgcg cgccatagcc gagagtatcg tcgaccacat
     1021 ggacaggacg aatcccatcc gcatgatgag cgactccgga gcgcggggaa gccgcagtca
     1081 gatggggcag atggccggaa tccgaggcct gatgtccgac ccttcgggcc gcatcatcga
     1141 ctacccgatc accgcgaact tccgcgaggg gatgaacatg ctcgagtact tcatctcgac
     1201 gcacggcgcc cgaaagggac ttgccgacac cgccctcaga accgccaagt cggggtatct
     1261 tacccgccgc ctggtggacg tggcgcagga cctgatcatc acgcagcacg actgcggaac
     1321 gaaccagggt gtgctcatcc gccccctgac gcaggacgga aaggtcatga ttccgctttc
     1381 cgagcgtatc tacggcagaa cgtcgctgca tgatatcgaa catccggaga ccggcgggat
     1441 gatcgtcgcg tcgggcgagt gcatcacgga cgagaaggcg cggatcatag agaaatccgg
     1501 cattgaagag gtctgggtcc gcagcccgct gagctgcgcg ctccgccacg gcatctgcca
     1561 ggtctgctac ggcatggacc tggccaccag aaaggaagtg ccgatgggag aggccgtcgg
     1621 cgtcgtcgcc gcgcagtcca tcggcgaacc gggaacgcag ctcacgatgc ggaccttcca
     1681 caccggcggc gtccgtctca cgggagagga catcacgcag ggtcttccgc gcatcgaaca
     1741 gctcttcgag gtccgccgtc cgaagaaggt cgccttcctc gcgggcgtgg acgggcgcgt
     1801 gaccgaagtc cgcgtcatgg aggggaagcg gaaggtcatc atcaccgccg agaacccgac
     1861 gggggacggc gaggaaaaag tcacgtacaa catccccatc aaccagaatc tcctggtgaa
     1921 cgaaggggac agcgtgctca agagcgacaa gctcaccgag gggaacttcg acccgcagca
     1981 gcttctcgag gtcgagggaa tcgaggcggt gcagcgcgcg ctcgtggatg aaatacagga
     2041 agtctaccgc tcgcagggcg tctcgatcag caacaagcac atcgaggtca ttctgcggaa
     2101 ggtggccccg gtgaaccgcg tgaagatcaa cgaggagggc gacacatcct tcgtcgccgg
     2161 ggatcttgtc tggacggacg acatcgacgc cgagacggcg gagatcggcc gcgagaacga
     2221 gcggaacatc gacgaggcgg tgcgcatctt cacggggata ccgttgaagg acgtgagcgc
     2281 ccagaagatg aatgaagccg ttctgcagca ccttggcaag ccgctcgacg aagaggcgat
     2341 ccggacgata ctccgccccg gaatgctgat ctcctccgcc atattgacgg atacggaagg
     2401 aaagcagttc aacctcgtgg tcggcgaagc cgccttccgg aagcagcttg aggggctcga
     2461 actcatcgaa gccttccagt ccgcggacgg gagaaggata gagccaggaa ctcggctgac
     2521 gctcggtcag ctcgccatcg tcacctccaa tgatccgacg cccctgctgg tgcgggacga
     2581 ggcgctgctt gaaaagctgg tcgacaccgc atacatcgcc gaggacattg tcgcggatgg
     2641 agagtccgtg gcgaaagccg acagagtctt tacgctggag gacgccgctc tttgcagaga
     2701 gcgcggcgtg aagagcgtga tggtatggca ctcggtggaa cgggtcaatc ttcttgatat
     2761 ccttgaggaa agcctgaagg agaagtgggg caagtcgctc acgcgcgcca tcgacattga
     2821 agggaacgct atcgccgatc ttccgcagct tgtcgacgag agagtaatca aggggattct
     2881 cgaagggcgt atcggggcgg ttgaaataga gggggcggtg ttttcaaggg atcacttcat
     2941 gaaagaactc ctctccgcca gagtctacgg aaagatactc cttgagcctg tcgtcgacgc
     3001 ccgaggaaat gtgctcgcgg aggcggcgca ggaggtcaat cagacggtca tcgaaagact
     3061 ctcaggcagc gccggcccga cggatgcctc cggaaacggt gtcaaggaac ttgttgtccg
     3121 cccgatggga gggcacaaga aagaagagaa gaagatcatc cagcgggtga ccttcgtccg
     3181 gaaactgcgc gaaggcccga aggtgaagcc cttcgtccac ggcatcacga aggcggcgct
     3241 tgccaccgac agcttcctca gcgcggcctc gttccagcag accgcccagg ttctcgcc
//
LOCUS       DAOPKU010000332         3285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_32723_length_3285_cov_4.636533, whole genome shotgun sequence.
ACCESSION   DAOPKU010000332 DAOPKU010000000
VERSION     DAOPKU010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32723_length_3285_cov_4.636533"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..494
                     /locus_tag="PK849_10205"
     CDS             225..494
                     /locus_tag="PK849_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66538.1"
                     /translation="MDEFGRSLRSFGLDPERVLSALRDGDVSAVLASLKKEEDMLLLR
                     NIELADLREQLLAMAGEEGRRKNDELSDDDVDLLSAAGQEPRSIP"
     gene            491..1282
                     /locus_tag="PK849_10210"
     CDS             491..1282
                     /locus_tag="PK849_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPE66539.1"
                     /translation="MKTREAFPSVLLIGQFAKLSGLSSRMLRFYAERNILVPAFTDNA
                     TGYRYYLPSQLIEAQLLRRLRQADFSLDDIAVILNGLETAQWGECVAAQRESITRKIA
                     AMKMALEELEHLEKHRGPEEYVLARFAPVRVLSAPLAGERLRLREIARTEAKRLFGED
                     GANAPLFCLYDLHEGPARSTICIPAEEPCGEIMPECVLAVSLHRGAYETVPATLEQLL
                     TWVKQNEFRTNGRVLERYFPSGEEDHPHGRVEIAVTLEGTPLPTA"
     gene            complement(1391..2203)
                     /locus_tag="PK849_10215"
     CDS             complement(1391..2203)
                     /locus_tag="PK849_10215"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HPE66540.1"
                     /translation="MFSMWKKMKMVVLAVLCAALYAALLIPFKGFVLIQGITEFRPAS
                     ALPVAMGLLFGPAGAWGAAIGNLVGDFFGSLSAGSLFGFVGNFMFAYVPYKLWINLGL
                     VPKNDREPDLKSKRKIAAYVTVAVLGSLACALVIAWELDVLGMVPFAALGSIISLNNS
                     IPAAVLGIPMLLFLYPVIKRWNLLWTDVMNEEDIPVAGGISGIGGVLMTVSILVGIIG
                     GFIRASNAGQAFLYRGLGAVGEVGSFGVFLVAGMGVAGLVLSGFLQSMPGKK"
     gene            complement(2271..>3285)
                     /locus_tag="PK849_10220"
     CDS             complement(2271..>3285)
                     /locus_tag="PK849_10220"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPE66541.1"
                     /translation="RILHHKAEGHKVFFISGRISGPIRRLKDATQRLSSGDLNAPLPP
                     ISGRDEVALLTVSFASMRENLLLYVERLKNETAARERIASELDIARSIQMSLVPRTFP
                     PFPKDGRIDLFARLEPAREVGGDFYDFFRTDEDHLMLVVGDVSGKGIPAALFMAVTRS
                     FVKAIAAASGGCPSPAELMAAVNDQIVEGNEACMFVTLSFVLVDLRDGSFRYAGGGHP
                     FPRLFGSEGAASLPPVKGPLVGAMGGMFFEEGSGKLKEGEGLFLFTDGVTEAFDADEA
                     LLSEDVMDRWLSEMRSLGSEALVSEMRRRISGFAGEAEQSDDITLLCFRYRGSETRTP
                     QAD"
BASE COUNT          744 a          986 c          946 g          609 t
ORIGIN      
        1 gacagtatag acattgacat cgtgtcaatg tcaagtgcga aatttaaaaa tttgttcctg
       61 gattgcttga aggagaaaaa gcttcatggc atactttgtg tactttgacg gcacccatcc
      121 gatgtcggcg gatcatcgaa gaaatccggg aggattgact ctcacatcgt gtgaaggtgt
      181 acgatgacgc cgttttcaat cgggtaccca aaggggggga aatgatggat gagttcggcc
      241 ggtcgctgcg ttcgttcgga ctggacccgg aacgggtcct cagcgccttg cgggacggag
      301 atgtcagcgc tgttctggcc tccctgaaaa aggaggagga tatgctcctt ctgcggaaca
      361 tcgagttagc cgacctccgc gagcagctgc tcgccatggc cggagaagag ggacgaagaa
      421 agaacgatga actttccgat gacgatgtcg atctgctgag cgcggcaggc caggaaccga
      481 gaagcatccc atgaagacga gagaagcgtt tccgtccgta ctgctcatag gtcagttcgc
      541 gaagctgagc ggcctatcga gccggatgct tcgtttttat gcggaacgga atatccttgt
      601 tcccgcgttc accgacaatg caacggggta tcggtactat ctgccgtcgc agctcatcga
      661 agcacagctg ctgcgacggt tgcggcaggc cgacttttca ctcgacgaca tcgccgtgat
      721 tctcaatgga ctggagacgg cgcagtgggg agagtgcgtt gctgcccagc gcgagtcgat
      781 cacccggaag atagcggcaa tgaagatggc gctggaggag ctggaacacc tcgaaaagca
      841 ccgggggccg gaggagtatg tgctggcccg tttcgccccg gtgcgcgttc tctccgcccc
      901 tctggccggc gaaagactgc ggcttcgcga gatcgcgcgt accgaagcta aacgcctctt
      961 cggagaagac ggcgcgaacg cgcctctttt ctgcctctac gatctgcacg agggaccggc
     1021 cagatcgacg atctgcattc cggcggagga gccctgcggg gagattatgc cggaatgcgt
     1081 gcttgccgtc tcgctccaca gaggagcgta cgagacggtt ccggccactc tggagcagct
     1141 tctaacgtgg gtgaaacaaa acgaattcag aacaaacggg cgcgtgctcg aacggtactt
     1201 tccaagcggg gaggaagatc acccccacgg aagggtggag atcgccgtca ccctggaggg
     1261 aacacctctt cccacggcct gaacggggca tatttctcaa cggagcggtt ccttccagcg
     1321 cagcgggaag gaaccgctcc cctatggcat gcgccgttcc cgcagcagac gaataaagaa
     1381 gacttctttt ctactttttt cccggcatgg actgaagaaa gcccgaaaga accagccccg
     1441 caacgcccat tccggcgacc agaaacacac cgaatgaccc gacttcgccg acggcgccga
     1501 gtcccctgta caagaacgcc tgtcccgcat ttgaagccct tatgaaacca ccgattatgc
     1561 cgacgagaat actgactgtc atgaggacgc cgccgattcc ggaaatgccg cctgctaccg
     1621 gaatatcctc ttcattcata acatcggtcc agagaagatt ccacctcttg atcaccgggt
     1681 agagaaagag cagcatggga atgcccagaa cggccgcagg aatcgaattg ttgagcgaga
     1741 tgatgctgcc gagggcagcg aaggggacca ttcccagcac atcgagctcc cacgcgatca
     1801 ccagggcgca tgcgagcgag cccagaacag cgacggtcac gtacgcggca atcttccgct
     1861 tcgacttcag gtcgggttcc ctgtcgttct tcgggaccag ccccaggttg atccagagct
     1921 tgtacggcac ataggcgaac atgaagttcc ccacgaaacc gaagagactg ccggcactca
     1981 gcgagccgaa aaagtcaccc acgagattcc ctatagccgc cccccaggcc cctgcgggac
     2041 caaataacag ccccatcgcc acgggaaggg cactcgcagg acgaaactcc gtgataccct
     2101 gaatcagcac aaagcccttg aaagggatca gcagagccgc gtacagagct gcgcagagca
     2161 ccgcaagcac aaccatcttc atcttcttcc acattgaaaa cacttcgttc aaaaagacca
     2221 ctccttcaag aggatatact atgctccgtc atatcaacat ccgaacgatc tcaatccgcc
     2281 tgcggcgttc gggtttccga tcccctgtag cggaagcaga gcagcgtgat gtcgtccgac
     2341 tgctccgcct cacctgcgaa gccggagatc ctccggcgca tctcgctgac aagcgcctcg
     2401 cttccaaggg agcgcatctc cgagagccag cggtccataa cgtcttcact cagcagggct
     2461 tcgtccgcat cgaaggcttc ggtcaccccg tcggtgaaga ggaagagtcc ctcaccctcc
     2521 ttcagctttc ccgagccttc ttcgaagaac attcccccca tagcccccac caaaggtcct
     2581 tttacgggag gaagcgaagc ggcgccttcg cttccgaaaa gcctcggaaa cgggtgtcct
     2641 ccgccggcgt accggaacga tccgtcccgg agatccacaa ggacgaagga aagggtaacg
     2701 aacatgcagg cttcgttccc ctcgacgatc tggtcattga ccgctgccat aagttcggcg
     2761 ggcgaggggc accctccgga agccgctgcg atggccttga cgaagcttcg cgtcaccgcc
     2821 atgaagagcg ctgcgggtat ccccttgccg gagacgtcgc ccacaacgag catcaggtga
     2881 tcttcgtccg tccggaagaa gtcgtagaag tctcctccca cctcgcgggc cggctcaaga
     2941 cgggcaaaga ggtctattcg cccgtccttc ggaaacggcg ggaaggttcg gggaacgagg
     3001 ctcatctgga tcgaccgggc aatgtccagc tcgctggcaa tgcgttcccg ggccgccgtc
     3061 tcgttcttca ggcgctccac atagagaagg aggttctccc tcatggaggc gaaggagacg
     3121 gtcaggagcg caacctcgtc acgccccgag atcggcggga ggggagcgtt caggtcgccc
     3181 gaggagagtc gctgcgtggc gtccttgagc ctgcgtatcg gtccgctgat ccttccggag
     3241 atgaaaaaga ccttgtgccc ttccgccttg tgatgcagaa ttctc
//
LOCUS       DAOPKU010000333         3284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_32738_length_3284_cov_5.379065, whole genome shotgun sequence.
ACCESSION   DAOPKU010000333 DAOPKU010000000
VERSION     DAOPKU010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32738_length_3284_cov_5.379065"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PK849_10225"
     CDS             <1..501
                     /locus_tag="PK849_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66542.1"
                     /translation="CEFVAKYGRVKAVPADIVVTSNAGYPLDQNLYQAVKSMTAGEAS
                     CTKGGVIIVAAECSDGHGGEAFYRTFETIPTPKGIMDMIMARGRNQTEPDQWQIQIFA
                     RVLMDFTVIMVTSAPKEMAEHLNMQWAPSLEEALSMAERILGKPDASVTVIPDGVAVI
                     VDAFSS"
     gene            602..1867
                     /locus_tag="PK849_10230"
     CDS             602..1867
                     /locus_tag="PK849_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPE66543.1"
                     /translation="MTDVRQDALHIAQKAIEAVLPENAVEAALRGEAFSARAQSGGRI
                     VLTAIGKAAWRMAKAAAETLGDRLDGGVVVTKYGHSNGPIKGVEIFEAGHPFPDENTI
                     KGTARALERVGGLSAEDTVLFLVSGGGSALFEKPGEGITLRDLVGVTDQLLACGADIV
                     EINTIRKRLSSVKGGRFAQFVAPAHVFSVVLSDVLGDRLDSIASGPAYPDGSTAADAL
                     RIVGKYGLSLSPVLQKALEKETPKSLENVTTLITGSVTALCKAAEREVAALGYAPLLL
                     TTTLNCEAREAGAFIAAMAKEIRSSGNPASPPCALLLGGETIVRLKGKGKGGRNQEMA
                     LAAAFGIKGLADTAVISVGSDGTDGPTDAAGGVVDGGTIGVLSGLGLDAEALLADNDS
                     YHALDACGGLVKTGPTGTNVNDLSILLCR"
     gene            complement(1947..3206)
                     /locus_tag="PK849_10235"
     CDS             complement(1947..3206)
                     /locus_tag="PK849_10235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE66544.1"
                     /translation="MERCGGVHFEKVVNKSPVTSFIWLPSENGKPCFVSEAIRSFGYA
                     PEDFTSGKIRYADLLHPDDVAAFSFSPGSEPQIYRISDSEGAWRWVFHRSCLIGEEAG
                     YPEELLWNISDITYYKEADEELRKNESKLKALMKAIPAILLVIDEDGRYIDVSGSSEA
                     ALVTASSELIGKTVHESFPNEKAEELMAPLKRCVETGAPQFAAYDIDIGGKKYFQEAR
                     ISLLPELVEGKRTVICVALDVTAQKELEEEILLQTGHNPIDGSSNRTHFNRAFLFSLA
                     EAQRTGGTLAFFMIDLDHFNTVNETIGREMADLLLKGIGQRLQKVCRQDDILYRMEGV
                     NYYLILPKPGTREDMGAVAERFLETIRGASSGYTGSFKLSATIGISVFPEDGQDAKTL
                     LRTAEAALSAGKAEGGNCYRFAEKPAR"
BASE COUNT          625 a          942 c         1063 g          654 t
ORIGIN      
        1 tgcgagttcg tcgccaagta cggcagggtc aaggccgttc ccgccgacat tgtcgtcacg
       61 agcaacgcgg ggtatcccct ggatcagaac ctctatcagg cggtgaagag catgaccgcg
      121 ggggaggcgt cgtgcaccaa ggggggcgtc atcatcgtcg ccgccgaatg cagcgacggt
      181 cacggcggcg aagccttcta ccggaccttc gagacgattc cgacgccgaa ggggatcatg
      241 gatatgatca tggcgagggg aagaaatcag acggagccgg atcagtggca gatccagata
      301 ttcgccagag tgctgatgga tttcaccgtg atcatggtga cctcggcccc caaggagatg
      361 gcggagcatc tcaacatgca gtgggccccg tccctggagg aggccttgtc gatggcggag
      421 cgaatcctcg gaaaaccgga cgcatcggtc accgtcatcc cggacggcgt ggcagtgatc
      481 gtcgatgcgt tctcttcctg aaaaaacagg gatgcgcgaa cgggctcgtt cggcggcgaa
      541 cggacgatgg cggagtgttt ctccgtgctg gcggcgaaat aaaaactgag gaggagaagg
      601 aatgacggat gttcggcagg atgccttaca cattgcgcag aaggccatag aggccgtgct
      661 cccggaaaac gccgtggagg cggcgctgcg cggagaggct ttttcagcgc gtgcgcagtc
      721 cggggggagg atcgtgctca ctgccatcgg caaagcggcg tggcggatgg cgaaggccgc
      781 tgcggagacg cttggagacc ggctcgatgg cggcgtcgtc gtaacgaagt acggacacag
      841 caacggcccg ataaagggcg tcgagatttt cgaagcgggg cacccgtttc cggacgagaa
      901 cacgatcaag ggcacggccc gtgcgctcga aagggtcggc ggactctccg cggaggatac
      961 tgtgctcttc ctcgtctccg gcggcggctc ggccctcttc gagaagccgg gcgaggggat
     1021 taccctccgg gatctcgtcg gagtgaccga ccagctgctc gcatgcggcg ccgacatcgt
     1081 cgagatcaac acgatacgaa agcggctctc ctcggtcaag ggggggcggt tcgctcagtt
     1141 cgtcgctccg gcgcacgtct tttcggtggt gctctccgac gttctcggcg acagactcga
     1201 cagcatcgcc tcgggcccgg cctatccgga cggttcgacg gccgccgacg cgttgcggat
     1261 tgtcgggaag tacgggctgt cgttgagccc ggtacttcag aaagctttgg agaaggagac
     1321 cccgaaatcg cttgaaaacg tgaccacgct cattaccggg agcgtgacgg cgctctgcaa
     1381 agcagcggag cgcgaggtcg ccgcactcgg ctatgcgccg ctcctgctga cgacgacgct
     1441 gaactgcgag gcccgcgaag cgggggcctt catcgccgct atggcgaagg agatccggtc
     1501 ttccggaaac ccggcgtcgc ctccgtgcgc cctgctcctc ggcggggaga cgatcgtccg
     1561 gctcaagggg aagggaaagg gcggacggaa ccaggagatg gctctggccg cggccttcgg
     1621 catcaagggg cttgcggata cggcggtgat ttccgtcggt tccgacggca cggacgggcc
     1681 gacggacgcg gcgggtggcg tggtggacgg cgggacgatc ggcgtgcttt ccggtctcgg
     1741 acttgatgcc gaagctctgc ttgccgacaa cgactcctat catgcgctcg atgcctgcgg
     1801 aggccttgtc aaaacggggc ccaccggaac gaacgtgaac gacctctcca ttctcctctg
     1861 caggtagtcg ctctttcggg tgcagacaga acgaggcccc tgaaaagggg cctcgcgtca
     1921 tattcatgtg cagtggcgga gaaaaattac ctggcgggtt tctcagcgaa tctgtagcag
     1981 ttgccgccct ccgcttttcc tgcggaaaga gccgcttccg ccgttctgag cagcgttttc
     2041 gcgtcctgcc cgtcttccgg gaagacgctg atgccaatgg tggcggaaag cttgaatgag
     2101 ccggtgtatc ccgaggacgc tcccctgatt gtttccagga agcgctccgc aacggccccc
     2161 atgtcttccc tggttcccgg cttcggcagt atgaggtagt agttgacgcc ctccatgcgg
     2221 tagagaatgt cgtcctgccg gcacaccttc tgaagccgct gccctatacc cttgagtaga
     2281 aggtcggcca tctctctccc gatggtctcg tttacagtgt tgaaatggtc gagatcgatc
     2341 atgaagaaag ccagcgttcc gcccgttcgc tgcgcctcgg cgagggagaa aaggaacgcc
     2401 ctgttgaaat gcgtcctgtt cgagcttccg tcgatcgggt tgtgtccggt ttggaggagg
     2461 atctcctcct caagctcttt ctgcgcggtg acgtcgaggg cgacgcagat cacggtccgt
     2521 ttgccctcca caagctcagg caggagggag atgcgggctt cctgaaaata ttttttcccg
     2581 ccgatgtcga tgtcataggc tgcgaactgc ggggcgccgg tctcgacgca gcgtttcagc
     2641 ggcgccatca gctcctccgc tttctcattc gggaacgact cgtggacggt ttttccgatc
     2701 agctccgagg acgccgtgac gagagccgct tcggaggaac ccgagacgtc gatgtaccgt
     2761 ccgtcttcgt caatgacgag gagaatcgcc gggattgcct tcataagcgc cttgagcttc
     2821 gattcatttt tccgcagctc ttcgtcggcc tctttgtaat aggtgatatc cgagatattc
     2881 cacaagagct cctccgggta gcccgcttct tcgccgatga ggcacgaacg gtgaaagacc
     2941 cagcgccacg ccccttccga gtcggagatc ctgtagattt gaggttcgga gccgggcgag
     3001 aatgagaaag cggcgacatc gtccgggtga agaagatcgg cgtatctgat tttcccggat
     3061 gtgaagtcct ccggcgcgta gccgaacgag cgtatggctt ccgatacaaa acacggtttc
     3121 ccgttttcgg aggggagcca gatgaaagaa gtcacgggac tcttgttgac aaccttttcg
     3181 aagtgtactc cgccgcatcg ctccatgccg gctttcatct ccctttagag gtatgtcccg
     3241 gagaatccgg aatgtttttc ttttcctcaa ggtattgtag cata
//
LOCUS       DAOPKU010000334         3278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_32831_length_3278_cov_3.547006, whole genome shotgun sequence.
ACCESSION   DAOPKU010000334 DAOPKU010000000
VERSION     DAOPKU010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32831_length_3278_cov_3.547006"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..357
                     /locus_tag="PK849_10240"
     CDS             229..357
                     /locus_tag="PK849_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66545.1"
                     /translation="METVRKENQGEKVFAEKGEKDDRFDEVMADMNQAAYRALLGQ"
     gene            475..1305
                     /locus_tag="PK849_10245"
     CDS             475..1305
                     /locus_tag="PK849_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPE66546.1"
                     /translation="MSNNGNDSASSSFIPALEVRDLRKSFGNVSVLEGISLSVREGEV
                     ISVIGPSGSGKSTLARCICRLEEIDGGEVLLYGDRIDNGKHSNRDIARLVGMIFQQFN
                     LFPHLSVLENIILSPVRILGNSPKAAEDKAMSLLERVGLCEKKSARPNELSGGQRQRV
                     AIARALAMNPKIMLFDEPTSALDPELVGEVLDVIADLAQSGMTMMIITHEMLFAKEVS
                     DRVLFMDGGKILEQGPPDSIFTSPQVPRTQLFLQRMLAHVGKELVQAHSDERTWTHDA
                     "
     gene            1295..1948
                     /locus_tag="PK849_10250"
     CDS             1295..1948
                     /locus_tag="PK849_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPE66547.1"
                     /translation="MTLDFSAITPYLHMLLRGAVITIQASSLSLLCGSILGILVGAIR
                     VSPFAPLRALAAGYIYVIRGTPLLIQLFLIYFGLPSLGLNLPAFTAGVIGLTINSSGY
                     VGEIVRGGIEAVPKGQWEASKVLGLSYVHTMRFIILPQAIRNMLPAIGNEFVTLIKES
                     SLLSTLAITELTMVGQQVRSVTYASFETFIMVGIIYLVMTSITSLALQYIEKRWTVD"
     gene            complement(2005..2943)
                     /locus_tag="PK849_10255"
     CDS             complement(2005..2943)
                     /locus_tag="PK849_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66548.1"
                     /translation="MIFELMSKGNLAQLPDEMCVVGLPETPSPRVLLLLPYNRYLLGT
                     VSLKTYEHWRLGKLGSEESEELILYDDKPKTITLGEVETITIAKEVVDRLKSCLRNQM
                     PPPGEHLPAMLILRGLLREDALFQDEDYLKNENAMQESLDKDAPARMAYWAIRFALSR
                     NDYEGVSRIKTWLKIAPDVFEGTLQTPRIWFSLMDLPGRKDIEEMESLAFSVDDLQRM
                     NSQSSRPVVLYSKAGYLILSDFGSDDAAFRIWLYLPIPLWNEMREKRKLSIREIVMAT
                     WGYLDGLAADTERSSFGLLASMTGGSGIGKATQGLS"
     gene            complement(3068..>3278)
                     /locus_tag="PK849_10260"
     CDS             complement(3068..>3278)
                     /locus_tag="PK849_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HPE66549.1"
                     /translation="LARVPVVPVVSYRIAPFRHKVIISSPVPMAEGLDRDDSIQENTE
                     RMNKIIEEMILRAPEQWLWLHRRWK"
BASE COUNT          794 a          819 c          808 g          857 t
ORIGIN      
        1 aatagcctac attcactttg ccagggaaaa aaaacgtcga gcctttgagg tggcgtttct
       61 ccacttgatc acttcctttc gtgttttctt tattctctcg tgaagaagaa atgtttgcaa
      121 gcctcttgcg tcgtgcgaaa aaagcgctat aataaaaaac aactatggta cggaccataa
      181 atatattata cacaacagac tgtgttgcac tgtaagggag gaaaaatcat ggaaactgtt
      241 cggaaggaaa atcaagggga aaaagtgttt gcggagaaag gcgaaaaaga tgaccggttc
      301 gatgaagtga tggccgatat gaaccaggcg gcctacaggg ctcttctggg gcagtagaac
      361 acgctcttcg tttcgggaga atatctgcgc gccggcattc gccggctttt ctgttttttg
      421 ccgactcgtc ctccggtaga gtataataac ggcagctttc gggaggtgag aggtgtgtcc
      481 aacaacggca acgatagtgc gtcatcatcg tttatacccg ctcttgaggt gcgggatctt
      541 cggaaatcat tcggcaatgt atctgttctt gagggcatct cgctttccgt ccgtgaagga
      601 gaagtcatct cggtgatagg tccgagcggt tcaggaaaga gtacgcttgc acggtgtatc
      661 tgccgtcttg aggaaatcga cgggggggag gttttgctct acggagacag gattgacaac
      721 ggcaagcatt cgaatcgtga tattgcccgc cttgtaggga tgatttttca gcagttcaat
      781 cttttccccc atctgtcagt tcttgagaat atcattttga gccctgtccg cattttggga
      841 aactctccga aggctgcgga agataaagcg atgtcgcttc tggagcgtgt aggactctgc
      901 gagaaaaagt cagcgcgtcc caacgagctc tccggaggac aacgtcaacg tgtagcaatc
      961 gcccgcgccc tcgcaatgaa tcccaagatt atgctcttcg atgagccaac cagcgccctc
     1021 gacccggaac ttgtggggga ggtgcttgac gttatcgccg atctagcaca gtccggaatg
     1081 accatgatga tcattacaca tgagatgctc tttgcaaaag aagtgtctga ccgggtgctc
     1141 tttatggacg gggggaaaat tctcgaacag ggacctccgg actcgatttt tacatccccg
     1201 caagttccga gaacacagct ttttcttcag cgaatgctcg ctcatgtcgg caaagaactc
     1261 gtacaagcgc attccgatga gaggacgtgg actcatgacg cttgattttt ccgcaatcac
     1321 tccgtatctt catatgctgc ttcgcggggc tgttatcact attcaggctt cttctctctc
     1381 tctgctgtgc ggatcaattc tgggaatact tgtaggcgct atcagggtgt ctccattcgc
     1441 tcctctgcgt gcgctggcgg ccgggtatat ttacgtgata agggggacgc cgctccttat
     1501 tcagctcttt ctcatttact tcgggcttcc ttcgcttggc ctgaatcttc ctgcctttac
     1561 ggcaggggtc atcggtctca cgattaactc gtccggatat gtcggtgaaa tcgttcgggg
     1621 aggcatcgaa gcagttccca aggggcaatg ggaggcttcg aaagtactgg ggctttccta
     1681 cgtgcatacg atgcgtttca ttattctgcc gcaggctatc cggaatatgc tcccggcaat
     1741 cggcaatgaa ttcgtcacgc tcataaaaga gtcatccctg ctctctacgc ttgcaataac
     1801 ggagcttaca atggtaggac agcaagtgcg aagcgtcacg tacgcatctt ttgaaacgtt
     1861 tatcatggtc ggcatcatct acttggtaat gaccagcatc accagccttg ctcttcaata
     1921 tattgaaaag cgatggaccg tagattgata actgatacca agaaaaaggc tgtgcctcat
     1981 cttccggcac agcctttttc tttctcagga gagcccctga gttgcttttc ctatgccgct
     2041 tccccctgtc atcgaggcca aaagaccgaa ggaagagcgt tccgtatcgg cggcaagtcc
     2101 atccagatac ccccacgtcg ccataacgat ttccctgatc gatagctttc ttttctcccg
     2161 catttcattc cacagcggga taggaagata gagccagata cggaatgccg catcgtcgct
     2221 gccaaaatcc gagagtatga gataccccgc tttgctgtag agcacgaccg gcctggaaga
     2281 ctgtgaattc atccgctgga ggtcatccac ggaaaaggct aggctttcca tctcctcaat
     2341 atctttcctt cctggtaaat ccatcagtga aaaccaaatg cgcggagtct gcagcgtgcc
     2401 ctcgaaaaca tcaggagcta ttttcagcca cgtttttatc ctggaaacgc cttcatagtc
     2461 gtttcttgaa agagcaaagc ggatggccca gtaggccatt cgtgccggag cgtctttatc
     2521 aagcgactcc tgcatggcgt tctcattctt tagatagtcc tcgtcctgaa aaagcgcgtc
     2581 ctccctgagc agtccgcgaa ggataagcat ggccggcaga tgctccccgg gaggaggcat
     2641 ttggttacgg agacagcttt tcaagcggtc tacaacctct tttgcaatcg ttatcgtttc
     2701 aacttcgccg agcgtgatag tcttcggttt atcatcatag aggatgagct cctccgactc
     2761 ctccgaaccc agtttcccaa gcctccaatg ttcgtaggtt tttaaagaaa cagtaccgag
     2821 gagataacga ttatagggaa ggaggaggag gaccctcgga gaaggagttt cgggcaagcc
     2881 gaccacacac atttcatccg gcagttgtgc aagatttcct ttcgacataa gctcgaatat
     2941 catatacatc cccccctttt aaaaatagag tagcattatt tcctaaaagc atcaagagaa
     3001 gaataccaca tccccctttt tatttcgcaa ttttcttttt tcagcattcc ggaacgaccg
     3061 ttttgttcta tttccagcgg cgatgcaacc aaagccactg ttcaggagca cgtaaaatca
     3121 tctcttctat aattttgttc attctctctg tgttttcctg aatggagtca tctctgtcga
     3181 gcccctccgc catggggaca ggagatgaga tgataacctt atggcggaaa ggagcgattc
     3241 tgtaggaaac aacgggtaca acaggtaccc gggcaaga
//
LOCUS       DAOPKU010000335         3262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_33043_length_3262_cov_3.241035, whole genome shotgun sequence.
ACCESSION   DAOPKU010000335 DAOPKU010000000
VERSION     DAOPKU010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33043_length_3262_cov_3.241035"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..564
                     /locus_tag="PK849_10265"
     CDS             55..564
                     /locus_tag="PK849_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66550.1"
                     /translation="MMFLVHDTLEERQEKIASYDDPFVAVLLAAADFEWTVRRAILAL
                     GISTTKEIKEDVLSRCSGLDKYKDAWKKEVEPLTGKGLSEIIPDWQYVKEKAYPLRNR
                     LVHGIDGVVTSQYAQERVKAILSASKAVAEFADSFGEPLYGRRIVRLKRRAKLRGSSS
                     AKKKQESRS"
     gene            655..>3262
                     /locus_tag="PK849_10270"
     CDS             655..>3262
                     /locus_tag="PK849_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HPE66551.1"
                     /translation="MKRRNGTLCFSAGDLCVFTESPFAAWMDRWYEETAGRAPSTLLF
                     AGESFEQCAPDAPDAEMRLLAGKGAEHERAFLEKLRNEGRSVADLAGERDPEATRRAL
                     LSGVDVLYQPYLEAPPFFGYADFLLRCGDGPRYEVCDAKLARSIKPVFPLQLCLYSEM
                     LAPLQGEIPERFHIALGSGETRSFRIERFLHLYRALRERFLAFQETFDPSAPPHPGDS
                     PAHGGWSGTAGRILERLDSLARVANIAGSQRKKLEKAGIVRMKDLAETREERVPGLSP
                     DVFARLREQASLQIASEGKERPEYRIVPSADGEELLPPASPGDVYFDIEGYPHAEGGL
                     EYLWGAVGTDTPAPSGEGEVVFYDWWAHDAAGEKAAFEQFIDWVRARRTLHPGMHVYH
                     YAPYETTAAKRLAAKYATREEEVDILLREGVFVDLYRVVRRALLVGTPGYSLKDVEHL
                     YREKRSGDVATGGASVVAYHEWTVSGESGDWRKSPLLRHIRDYNEEDCRSTMDLGLWL
                     REHLKEERVAPLDSPGESAVPSEREKTVRNEKNERRKELRRSLPGVLLEDVRGHADRP
                     AAELLAGLVGYHWREAKPVFWRMYDRLARTGAELADDPDCLGMLRIAGEPRPDEKRSL
                     IYPYTFPPDQETKIEQGKRCFANTTPTLSVEVHSLDTERGFVELRLGARNEPLPPLLD
                     LVPDEYVGAETIEDSLHGIAQRWTEGTPPCGALRDLLDAAPPKLSDGRPFLYRPEDGV
                     EGVLRLARDLGDSVLALQGPPGSGKTFTASRIIADLAASGQRIGVTANSHKAIMNLLA
                     AVVEALDERGITAQVVKAGAGADEPLVVNGRILPCKNVDLAKHIPEGAPVVLGATAWG
                     FCRPEMEGTLDCL"
BASE COUNT          637 a         1031 c         1020 g          574 t
ORIGIN      
        1 aggaatatga agatttaata atacaataat tgcgggttta caaaaggaga gtttatgatg
       61 tttcttgtcc acgatacgct tgaagagagg caggagaaaa tcgcttctta cgatgatcct
      121 tttgttgccg ttctcctggc ggcagcggat ttcgaatgga ccgtcaggcg tgcgattctt
      181 gccttgggca tcagcactac gaaagagatc aaggaggatg tgctgagtcg gtgctccggt
      241 cttgataaat acaaagatgc ttggaagaag gaagttgaac cgttgaccgg gaaagggctt
      301 tcggaaataa taccggactg gcagtatgtc aaagaaaagg cctatccgct gcggaaccgc
      361 ctcgtccatg ggatcgatgg cgtcgttact tctcaatatg cgcaggaaag ggtaaaggcg
      421 atcctttcgg caagcaaggc ggtcgcggag ttcgccgact ccttcggtga acctctttat
      481 ggccgcagaa ttgtcagatt aaaacgccgc gcgaagttgc ggggttcctc ttctgcgaaa
      541 aagaagcaag agagccgctc ctgaacgaca acggttctct gcgccgttcc ctgcaaacgg
      601 acgctttgcc ggcgcacttc ctggatcccg aatcaagccg aggagatgat gctcttgaaa
      661 cgccgcaacg gaacgctgtg cttttccgca ggagatttgt gcgttttcac cgaaagcccc
      721 ttcgccgcat ggatggaccg ctggtacgaa gagacggccg gacgcgctcc ctcgacgctt
      781 cttttcgccg gagagtcttt cgagcagtgc gctccggatg caccggacgc cgagatgcgt
      841 cttctggcgg gcaagggcgc cgagcacgaa cgcgccttcc tcgaaaagct gcggaacgaa
      901 gggagaagcg tcgccgatct cgcgggggag cgcgatcccg aggcgacccg gcgagccctg
      961 ctctccggcg tcgacgtgct ctaccagccg tacctcgaag cgcctccctt cttcggatac
     1021 gcggacttcc tgctccgttg cggcgacgga ccgcgctacg aagtttgcga cgccaagctg
     1081 gcccgttcga tcaaaccggt cttccccctt cagctctgcc tctactccga gatgctcgct
     1141 cccctccagg gggagattcc cgaaaggttt catatcgccc tcggttccgg cgagacgaga
     1201 agcttccgga tcgagcgctt tctccacctc taccgcgcgc ttcgcgagcg ctttctggca
     1261 tttcaggaga cgttcgatcc gtcggcgccg ccgcatccgg gagactcccc ggcgcacggt
     1321 ggatggagcg ggacggcggg gcgcattctc gagcggctcg actcgctcgc ccgcgtcgcc
     1381 aacatcgccg gatcgcagcg gaagaagctg gagaaggccg gaatcgtgcg aatgaaggat
     1441 ctggccgaaa ctcgggaaga gcgcgtcccc ggtctctccc ccgacgtctt cgcccgcctc
     1501 cgcgagcagg cttccttgca gatcgcctcc gaaggaaagg agcggccgga gtatcgcatt
     1561 gttccgagcg ccgacgggga ggagcttctt cctcccgcct cccccggcga cgtctatttc
     1621 gacatcgaag gctacccgca cgccgaaggc ggactcgaat acctctgggg cgccgtcggc
     1681 acggacacgc cggccccttc gggggagggt gaggtcgtgt tctacgactg gtgggcgcac
     1741 gacgccgccg gagagaaggc cgccttcgaa cagttcatcg actgggtccg cgcgcgcagg
     1801 acgctccacc ccggaatgca cgtctaccac tacgccccgt acgagaccac ggcggccaag
     1861 cgacttgccg cgaagtacgc cacccgggag gaggaagtcg acatcctcct ccgcgaagga
     1921 gtgttcgtcg atctctaccg cgtcgtccgg agagcgctgc tggtgggaac tccgggctac
     1981 tccctgaagg atgtggagca cctctaccgg gagaagcgct ccggagatgt ggcgacgggc
     2041 ggcgcttccg tggtcgccta ccacgagtgg actgtaagcg gcgagtccgg agactggagg
     2101 aaatccccgc tgcttcggca cattcgcgac tacaacgagg aggactgccg ctccacgatg
     2161 gatctcgggc tctggctccg cgagcatttg aaggaggagc gcgtcgctcc gctggactcc
     2221 cccggggaga gcgccgttcc ttcggagagg gagaagactg tgcggaacga gaagaacgaa
     2281 cgacggaagg agcttcgccg gagtcttccc ggggtcctcc tcgaagacgt ccgcggccac
     2341 gccgaccgcc cggctgcgga acttctcgcc ggactcgtgg gctaccactg gcgcgaggca
     2401 aaacccgtct tctggcgcat gtacgacagg ctggcgcgca ccggcgccga actggcggac
     2461 gatcccgact gcctcggtat gcttcgcatc gccggggagc cccgaccgga cgaaaagaga
     2521 tctctgatat acccctacac attcccgccc gaccaggaga cgaagataga gcagggcaag
     2581 cgctgcttcg cgaacaccac ccctacgctg agcgtcgaag tccactcgct cgacaccgaa
     2641 cgaggcttcg tcgaactgcg gctgggagcg cggaacgaac cgctcccccc tctcctcgac
     2701 ctcgtgccgg acgaatacgt cggcgccgaa acgatagagg actcgctcca cggaatcgcg
     2761 cagcgatgga cggaggggac gccgccctgc ggagcgcttc gcgaccttct cgacgccgcg
     2821 cctccaaaac tctccgatgg acgccccttt ctctaccgtc cggaggacgg tgtggaaggg
     2881 gtgctccgcc tcgcacgcga tctcggcgat tccgtcctcg cgctacaggg tcctccgggt
     2941 tccggcaaaa ccttcacggc gagcagaatc atagccgacc tcgccgcctc cggacaacga
     3001 atcggcgtga ccgccaacag ccacaaggcg atcatgaacc ttctcgcggc cgtcgtggaa
     3061 gcgctggacg agcgggggat cacggcccaa gtcgtcaagg cgggagccgg cgcggacgag
     3121 cccctcgtgg taaacgggcg aattctcccg tgcaaaaacg tcgatctcgc gaagcatatc
     3181 ccggaagggg ctcccgtcgt cctcggagcg acggcgtggg gcttctgccg ccccgagatg
     3241 gagggtacgc tcgattgcct tt
//
LOCUS       DAOPKU010000336         3257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_33105_length_3257_cov_4.504060, whole genome shotgun sequence.
ACCESSION   DAOPKU010000336 DAOPKU010000000
VERSION     DAOPKU010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33105_length_3257_cov_4.504060"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK849_10275"
     CDS             complement(<1..568)
                     /locus_tag="PK849_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66552.1"
                     /translation="MFSAFPPFLWYYLKGGIRLGHTLERLPPKTIEKVFFVFMLSAGL
                     VLVVFFISGLFSSRGVSSSSDYAVANRKAGVRGVSGVILGSLVGGAATVGTVQMAYQW
                     GLSALWFTLGSGAACLLMGLWFVTPLRSVELITLPQYLGRHFGKRTALLVAVSTASGS
                     FLSVIAQFLSGVALMRSVFPVSTGVAALL"
     gene            594..779
                     /locus_tag="PK849_10280"
                     /pseudo
     CDS             594..779
                     /locus_tag="PK849_10280"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(869..1159)
                     /locus_tag="PK849_10285"
     CDS             complement(869..1159)
                     /locus_tag="PK849_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011114071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HPE66553.1"
                     /translation="MRSVRQTRQTAAFKRDFKREIKRASRHILKERLWGVVAALTMGS
                     PLDPSYRDHPLGGNWRGHRECHIAPDLLLLYYLSEDALTLVRLGSHAELFGL"
     gene            complement(1146..1430)
                     /locus_tag="PK849_10290"
     CDS             complement(1146..1430)
                     /locus_tag="PK849_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HPE66554.1"
                     /translation="MGARVTKTKRPGFRSVNVRLVARDKELAENIFRKVGLTPTEAIR
                     LFYRQTINTGGIPFGLVLPEETLAAMREAESGTAEEISLDDLKAELDAIC"
     gene            complement(1541..1822)
                     /locus_tag="PK849_10295"
     CDS             complement(1541..1822)
                     /locus_tag="PK849_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66555.1"
                     /translation="MDIVTKIRNLCSSEVDWVKDAGKAFADLADDLFDLFAERKAPSP
                     EFKVRLARTFSDVNSALAACGMEKIEGESEREIAAAYFAYCKGVIESRM"
     gene            2254..2475
                     /locus_tag="PK849_10300"
     CDS             2254..2475
                     /locus_tag="PK849_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HPE66556.1"
                     /translation="MKCHVCGAGMQEMKTDLPFKINERSIIVVRDVPVIQCSGCREYM
                     LSDEVMERLDELLAGVNSNADLEIVRFAA"
     gene            2586..2915
                     /locus_tag="PK849_10305"
     CDS             2586..2915
                     /locus_tag="PK849_10305"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HPE66557.1"
                     /translation="MKSDAPTEALRRIQECVKRRDILWTYHVFMRMQGRCIPRSSILD
                     SVASYEIIESYPEDKYMASYLVRAECGADVVHVLFAFDIADKFVRIITTYRPDVALWS
                     EDGRKRR"
BASE COUNT          785 a          904 c          887 g          681 t
ORIGIN      
        1 taagcaacgc cgcgacgccg gtcgagacgg ggaagacgga acgcatcaac gccacgccgg
       61 agagaaactg cgcgataacc gagaggaacg agcccgaagc cgtggaaacg gccaccagga
      121 gcgccgtccg tttgccgaag tgccgcccga ggtactgcgg cagcgtgatc agctctaccg
      181 agcggagcgg cgtcacgaac cagagcccca tcaggaggca cgcggccccg ctgccgaggg
      241 tgaaccagag cgccgaaagc ccccactggt acgccatctg caccgtaccg accgtagcgg
      301 ccccgccgac gagcgagccg aggatgacgc ccgagacgcc gcgcacaccc gcttttctgt
      361 tggcgacggc gtaatcggag gatgaggaga cgcctctgga ggagaacaga ccggaaatga
      421 agaagacaac gagcacaaga cctgcggaga gcatgaacac gaagaacacc ttctcgattg
      481 tttttggagg aagccgctca agcgtatgac cgaggcggat accccccttg agatagtacc
      541 atagaaacgg aggaaacgcg gagaacatgc agtaggccgc caaccttcgt ctccgagcgg
      601 aacttatgga cgagatcgtc tcgttcgtcc tcaaacagaa gtggaaccgc gaggaagccg
      661 cgcgtcgttg cagcgttacg tgcccgcgaa tggacgaatt actggaggga acgctctccg
      721 aattctctct cgacgccttg atcaacttgc gacatcgctc gggctgccgc tccgaataaa
      781 ggtcggcgta taaaccgccc ctggaggaga acagaccgag aaggaagaag acagcgagga
      841 ctcgttcttt acaacatgct tgtcatcttc aaagtccgaa aagctccgca tggcttccga
      901 gacgcaccaa ggtaagagca tcttccgata gataatagag caacagcaga tccggggcga
      961 tgtgacactc cctatgtcct cgccagtttc ctccaagcgg gtgatcgcga tacgacgggt
     1021 cgagagggct ccccatcgtc aacgcggcga cgactcccca aagacgctcc ttgagaatat
     1081 ggcgcgaagc cctcttgatc tcccgtttaa aatcgcgctt gaacgccgct gtttgcctag
     1141 tttgcctaac agatcgcatc cagctcggcc ttcagatcat ccagcgatat ttcttccgcc
     1201 gtgccggact cagcttcccg catggcggcc agagtttctt ccggtaaaac gagcccgaaa
     1261 gggatgcctc ccgtgttgat ggtctgccgg taaaagagac gaatcgcctc cgtgggagtc
     1321 aaaccgactt tcctgaagat attctccgcc agctccttat cccgggcaac aagccgaaca
     1381 ttgacactgc ggaatccggg acgcttggtt ttcgtaacgc gcgcacccat aaaaccacct
     1441 cctccacagg gaataataac gccataagcg ttacaagtca aaaattcata aatgcctttt
     1501 cgttcgcatt actttgtggt ggttcttcca gatgcgcaac ctacattctg ctctcgatga
     1561 cacctttgca ataggcgaag tacgcggcgg cgatctcgcg ttccgattct ccctctattt
     1621 tttccatgcc acaggcagca agcgcgctgt tcacatccga gaaagttcgc gccaacctga
     1681 ccttgaactc cggagacgga gcctttcgtt cggcgaaaag atcgaacagg tcgtccgcca
     1741 aatccgcgaa cgcttttcca gcatccttca cccagtcgac ttcggatgaa cagagatttc
     1801 gtatcttggt gacgatatcc attactctcc tcctttcgac gaagcaatag catgcgtacg
     1861 caaaacctct ccgggtaagg ggctggattt ggggttggtc caatagccgc acaatggatt
     1921 acacggctcc ctcgcccatc tactttctga ggaacagagg cgctatgtct tggtaaagat
     1981 agcccttccg ctcccccttc gcaagtccac gggcgagtct cgccgctcgg aatcgatagg
     2041 gttgcgaatc gttctgcatg ggatcgctat tcgcgtctcc ttcgatccga aaggctgttt
     2101 tctcgatgat ataatatgat aacaattttg tggcaaccat tcgcaagccg atgagaaaac
     2161 tgatttctga cgaacggaat actttcgcct cgagaaggcg ctccatgtct gaccagaacg
     2221 ttgcgccggc gaaaatggac ggagggaaaa acgatgaaat gtcatgtttg cggagccggg
     2281 atgcaggaga tgaaaaccga cctccctttt aagatcaatg aacgttccat cattgtcgtt
     2341 cgcgatgtcc cggtgattca gtgttccgga tgccgggaat acatgctttc cgacgaggtt
     2401 atggagcgcc tcgacgagct gctcgccggc gtgaactcca acgccgactt ggagatcgtt
     2461 cgtttcgctg cttgaaacgg aatgttgaat aatgtcggag cctgttatag cgtaacacgg
     2521 gcaaagcaac ctcttcgggg tgcttcacaa aatgcttttc ttcttattcc ggaagggttt
     2581 ttcaaatgaa gagcgatgct ccaacagaag cgcttcgacg aattcaagaa tgtgtgaaac
     2641 ggagagatat cctctggaca taccatgtgt ttatgcgtat gcaaggccgt tgcataccac
     2701 gaagcagcat tcttgactcc gttgcttctt acgaaataat agaaagttat ccggaggata
     2761 agtatatggc cagctatctt gtacgagccg aatgcggcgc ggatgtcgtg catgttctct
     2821 ttgcattcga tatcgcggac aagttcgtcc gcattataac gacgtacaga ccggatgtgg
     2881 ccctctggtc cgaagacggc aggaagaggc ggtaagttaa aataaagtta accagcatga
     2941 acgcgctttt ttcaagtatc tctccgctgt ttcaggaaca catcctgtga atagaaatgt
     3001 tcgcttttac cgacgatatg cgtggtacga tgttctccgc gcacgcgaac atcgcccggc
     3061 ggtatcgggt attcacgctg cggaaggaga gggcagagtg aacgaagcaa cgacgacagc
     3121 gcgcatgact gcggagccgc tccgcctgct ctacgaggat gagtggttcg tcgccgtgca
     3181 caagccggag gggctgctgg tgcaccgttc gccgctcgac cggcatgaga cgcggttcgc
     3241 gctccaggag gcgcggg
//
LOCUS       DAOPKU010000337         3251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_33178_length_3251_cov_6.932728, whole genome shotgun sequence.
ACCESSION   DAOPKU010000337 DAOPKU010000000
VERSION     DAOPKU010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33178_length_3251_cov_6.932728"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PK849_10310"
     CDS             complement(<1..381)
                     /locus_tag="PK849_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE66558.1"
                     /translation="MDGRAEHGDAPARNVLVVEDNEDLRSLIVRRLHRAGYEAFGVSL
                     GCEAVEKAFERPGTALLIDHNLPDMSGQELVALLKERGAAPPFIIMTGQGDERLAVQM
                     MKLGAFDYLVKNSELLDKLPETLGR"
     gene            complement(402..848)
                     /locus_tag="PK849_10315"
     CDS             complement(402..848)
                     /locus_tag="PK849_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE66559.1"
                     /translation="MHNEVIILIAEDDEGHAGLIRKNLARAGISNHVEHFKDGQEIID
                     FLFRIGNGPHRRSHTPYILLLDIRMPKLDGTEVLKRIKEDHELRKMPVIMITTTDDPR
                     EVERCHELGCSSYITKPVEYDAFVNAVRQLGLYLAVVQVPQVNGVH"
     gene            complement(869..>3251)
                     /locus_tag="PK849_10320"
     CDS             complement(869..>3251)
                     /locus_tag="PK849_10320"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HPE66560.1"
                     /translation="YAERGVLFDAIICTDDDALVFLRERGESIFGNVPKVFCGINDFT
                     PEHIKGMTNVTGVNESISVRETIEAALNLHRSTKTIAVVSGSRRTEKRNLELVRQSAP
                     SFSDAVQFIWLDELEPHALQKRLGELNPESSAVLMLAYLLTPSGQALTVRENMELITE
                     ATSVPVFGCWDFLLPHGAVGGKVVHGHSQGAAAASLALRILAGERADSIPVVLESPNR
                     YVFNAAMLQKFAIPEALLPPDSQIVYRTAASLLSNWETLSRRLFFAYDLFAKNTTPML
                     LIDPDTGIILDGNHSARGFYGYPELKGMNIAQINTLSPEEVSREMERARSRNNTSFDF
                     KHRLADGSVRDVGVHSSPVFIDGREILLSLVIDETERVTAQEALGQRNRWIVALVVSA
                     LLLQSVVLLQLLRSAAQRKKAEKALRESERKLSALFASMTEVVALHDLVFDEEGRPAN
                     YRITGCNAAFTKSIGIPEEAAVGRLATEVYGTEKAPYLQEYGKVAMTGEPSTFETYFA
                     PLDKHFFISVVSPGENSFATVTTDITSIKKAEQLIEAKNKELEQVVYVASHDLRSPLV
                     NVDGYSRELEYSVEDLLRALDDESLAPPERERAIRALLPDMTDSLRRIRGSAKQMDAL
                     LRGLLKLSRLGRAALSVGSVDMNELMAELSSSLQFQVREAGAALRVGRLPSCRGDAVQ
                     LTQVFANLLGNALKYLDPQRPGEIVVEGRIEQDRAVYLVRDNGIGIAPQHQEKIFQLF
                     HRLNPSANEGEGLGLTIVRQILGRLDGEVVVESTPGTGSTFTITLPAASKKGE"
BASE COUNT          590 a         1014 c          962 g          685 t
ORIGIN      
        1 gcgccctagc gtctccggca gtttatccag cagttcggag ttcttcacca gataatcgaa
       61 ggcgcccagc ttcatcatct gcactgcgag tcgctcgtct ccctgtccgg tcatgatgat
      121 aaaaggcggc gccgcgccgc gctctttcag cagcgcgacc agctcctgtc cggacatgtc
      181 gggtaaatta tgatcgatca ggagcgccgt tccgggccgc tcgaaggctt tctccacagc
      241 ctcgcatccg agggacacgc cgaacgcctc gtaccccgct ctgtgaagcc ttctgacgat
      301 caggcttcgc aagtcttcgt tgtcctctac gacgagaacg tttcgtgccg gggcgtcccc
      361 gtgctcggcg cgtccatcca tccttcgggt ctccgccgct ctcagtgcac gccgttcacc
      421 tgaggcacct gcacgactgc aaggtagagc cccagctggc gaacggcgtt gacgaaggcg
      481 tcgtattcca caggtttcgt gatgtagctg ctgcacccca gttcgtgaca gcgttccact
      541 tcccggggat cgtcggtcgt ggtgatcatg atcaccggca tcttacggag ttcgtggtcc
      601 tctttgatgc gcttgagcac ttccgtgccg tccagcttgg gcatccggat gtcgagcagc
      661 agaatgtacg gggtgtgcga ccgcctgtgc ggcccgttcc cgatgcgaaa gagaaaatcg
      721 atgatctcct gcccgtcctt gaagtgttcc acatggttgg agattcccgc gcgcgccaaa
      781 tttttccgta tcagcccggc gtgcccctcg tcgtcctcgg ctatgagtat gatgacttca
      841 ttgtgcatgc gttcttcctc ctccataatc attctccctt tttcgacgct gcgggcagcg
      901 tgatcgtgaa cgtgcttcca gttccgggcg tcgattccac gacgacctcc ccgtccagtc
      961 ttccaaggat ctgcctgacg atcgtcaggc caagcccctc gccttcgttc gccgatggat
     1021 tcaacctgtg gaagagctgg aaaatcttct cctggtgctg cggcgcgatc ccgataccgt
     1081 tgtcccgcac caggtagacg gcgcggtcct gctcgatccg cccctcgacg actatttccc
     1141 ccggacgttg gggatcaaga tacttcagcg cgttgcccag aaggttcgcg aaaacctgag
     1201 tgagctgcac ggcgtcgcct ctgcacgagg gcaacctgcc gacgcgaagc gccgccccgg
     1261 cctctctgac ctgaaattgc agagaggaac tcaattccgc catcagctcg ttcatatcga
     1321 cggatccgac gctgagcgcc gcccgcccga gccgcgaaag cttcaagagg cctcgaagga
     1381 gcgcgtccat ctgcttcgcg cttcctctga tacggcgcag agagtcggtc atgtcgggga
     1441 gcagggcgcg tatcgcgcgc tcccgttccg gaggagcgag agattcgtcg tccagcgcgc
     1501 gcagaagatc ctccacggag tactccagct cgcgactgta tccgtccaca ttgaccagcg
     1561 gggaacgaag gtcgtgcgac gctacgtaga cgacctgctc cagttccttg ttcttcgctt
     1621 cgatgagctg ctccgccttt ttgatcgagg tgatgtcggt cgtgacggtg gcgaagctgt
     1681 tttcgcccgg agagacgacg gagatgaaga agtgcttgtc cagaggggcg aagtacgtct
     1741 cgaaagttga gggttctccc gtcatggcga ccttgccgta ctcctgtaaa tacggagcct
     1801 tctccgtacc gtagacctcg gtcgccaggc gtccgacggc cgcttcctcc gggattccga
     1861 ttgactttgt gaacgcggcg ttgcacccgg taatccggta gttcgcagga cggccttcct
     1921 cgtcgaagac caggtcgtgg agcgcgacga cctccgtcat cgaggcgaag agcgcgctga
     1981 gtttccgttc gctctcgcga agcgcctttt ccgccttctt tctttgcgcg gcgcttcgca
     2041 gcagctgcag cagcacgacg gactgaagca gcagggcgga aacgacaagc gcgactatcc
     2101 agcggtttcg ctgccccagc gcttcttgcg cagtcaccct ctcggtctcg tcaataacca
     2161 gggagagtaa gatttcccgg ccatcgatga acaccggcga ggagtgcacg ccgacgtcgc
     2221 gaacgcttcc gtcggccagg cgatgcttga aatcaaagga agtgttgttc cggctgcgcg
     2281 ccctctccat ctccctggaa acctcctcag gggagagcgt gttgatctgc gctatattca
     2341 tccccttcag ctccggatag ccgtaaaagc cgcgggcgga atggtttccg tcgagtatga
     2401 tccccgtatc cgggtcgatg agcagcatgg gcgtggtgtt cttcgcgaag aggtcgtagg
     2461 cgaagaagag ccttcgggac agcgtctccc agttcgaaag gaggctcgcg gcagtgcggt
     2521 agacaatctg cgaatccggg ggaagcaacg cctcggggat tgcgaatttc tgaagcatgg
     2581 cggcgttgaa gacgtagcgg ttcgggcttt cgagcactac cggtatggag tccgcgcgct
     2641 ccccggcgag aatgcgaagc gccagcgacg cggcggccgc accctgagag tgtccgtgca
     2701 cgaccttgcc cccgaccgcc ccgtgaggga gcagaaaatc ccagcatccg aagaccggga
     2761 cggaggttgc ctccgtaatg agctccatgt tctcgcgaac ggtaagcgcc tgccccgacg
     2821 gggtgagcag atatgccagc atgagcacgg cggatgattc cgggttcaat tcgccgagac
     2881 gtttttggag cgcgtgcggt tccagctcat ccagccagat gaattgcact gcgtcgctga
     2941 aggagggggc gctctgccgc accagctcca gattgcgctt ctcggtgcgc cgagagcccg
     3001 agacgacggc tatcgtcttc gtcgaacggt gcaggttcaa agctgcctcg atcgtttccc
     3061 tgaccgaaat ggactcgttg acccccgtga cgttggtcat ccccttgatg tgctccggag
     3121 taaaatcgtt gatgccgcaa aagaccttgg gtacgttccc gaagatcgat tctcccctct
     3181 cccgcagaaa gaccagagcg tcgtcgtccg tacagatgat cgcgtcgaag aggacgccgc
     3241 gttccgcata c
//
LOCUS       DAOPKU010000338         3240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_33335_length_3240_cov_4.569231, whole genome shotgun sequence.
ACCESSION   DAOPKU010000338 DAOPKU010000000
VERSION     DAOPKU010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33335_length_3240_cov_4.569231"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PK849_10325"
     CDS             complement(<1..946)
                     /locus_tag="PK849_10325"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HPE66561.1"
                     /translation="MESKKKILWYSFLIAIFILPLSGCSLSGRTNSYEEARALSEEAY
                     IFAYPMLENYRTMQVQAITSGNFNRFVHSTELLGPEDINALRPHNDTLSSTAWLDLRA
                     EPMVLTAPPLPDRYYSFQFVDLYTSNFAYVGARTIGSSGGVFLIAGPEWNGKTPRTVD
                     ALFQSEGNFVLCFGRTALNGPDDLPNVRAIQQEYTLQPLSKFLGTPPPEEDPSEVFPL
                     YVKIAADSVHFISYLNFLMGQAKVHPSEESLFERFGLLGIGPSFYFNANDFDATIRDA
                     IDDGIVSALEQIEESQSLLHEERNGWTIFRQIFGDRERL"
     gene            complement(1237..2202)
                     /locus_tag="PK849_10330"
     CDS             complement(1237..2202)
                     /locus_tag="PK849_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE66562.1"
                     /translation="MKNRKMIIAAIALVSLFVFTGSALAVTHITMGTAGVGGMNYPVG
                     MAMAKIWNANIKDMKAVAIATAGAVQNIDMIRTEDIEVAVCRANEAYRAYNGIEKYKE
                     PHTWLRALTGGVMFDAKQVLALKDSGITSIADFRGKRVAVGPVGSGGELDAREILAAY
                     GLTYDDMTPEYVEASQAVDMMQDGLIQGAILGFTPGASAISELMVTDKVIILPIDDAG
                     YQSLKKINPFVSKRVLPAGTYPNQFYDVETCGDPADLIITRIDVPEDLVYQMTKSLYE
                     NAGDVRAVAAAVRQFGPDLVAPSEEMLIPYHPGALRYFKEAGILK"
     gene            complement(2287..>3240)
                     /locus_tag="PK849_10335"
     CDS             complement(2287..>3240)
                     /locus_tag="PK849_10335"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE66563.1"
                     /translation="QVDLRARKIGLTGLSKEELPDVKKTLLRYGHMFLPIIILVVLLM
                     QHFTPLYAAFYSILVTWLLSFLRKETRMGVGKLVPMAVASARSCLSLSVAMANAGFVV
                     AVLSMTGIGIILADNIVALSHGSLFITLVLCMVVSIILGMGLPTSACYIIAATIAVPI
                     LHQLNVPGIQAHFFVFYFACLSTVTPPVALASYVAAGMSGAGTNEVGWAAFKLALAGF
                     IVPFFFVYAPAILLQADSSFVIAWAAVSGLIGAALLSVAIEGFLFIEIPWFLRIIFFG
                     AALTLIAPGASSDAAGFALATVGILSVLYLRRRKGDAAAGL"
BASE COUNT          770 a          922 c          899 g          649 t
ORIGIN      
        1 gtaagcgctc cctgtcaccg aagatctgcc tgaagatcgt ccagccgttt ctctcctcgt
       61 gaagcagcga ctgcgactct tcgatctgtt caagcgcaga gacgatcccg tcgtcgatcg
      121 catcccggat cgtggcatca aaatcgttcg cattgaaata gaaggacgga cctataccca
      181 aaagaccgaa acgttcgaac agcgactctt cggaggggtg caccttcgcc tgccccatca
      241 gaaagttcag ataggagatg aagtgaacgg aatccgcggc gatcttcacg tacagcggaa
      301 agacttccga gggatcctcc tcgggggggg gagtcccaag aaacttactc agaggctgga
      361 gcgtgtactc ctgctggatc gcacgtacat tcggcaggtc atccgggccg ttgagagccg
      421 tacggccgaa acagagtaca aagttccctt cggactggaa gagagcatcg acagtacgag
      481 gcgtcttccc gttccactcg ggaccggcga tcaggaaaac ccctccagac gaaccgatag
      541 tcctggcccc gacgtaggcg aagttgctgg tatagaggtc gacgaactgg aacgaataat
      601 agcggtccgg gagaggcggc gccgtgagca ccatgggctc ggcccgaaga tcgagccagg
      661 cggtcgagga aagcgtgtcg ttatgaggcc gaagcgcatt aatatcctcc gggccaagaa
      721 gttccgtaga gtgaacgaac cggttgaaat tgcccgaagt gatggcctgc acctgcatcg
      781 ttctgtagtt ttccagcata ggataggcga agatatacgc ctcctccgaa agagcgcgcg
      841 cctcttcgta gctgttcgtc cggcccgaaa gagaacaccc ggagagcggc aggatgaaaa
      901 tcgctatcaa gaacgaatac cagagtattt tcttcttgct ctccacggcc aatgctccct
      961 tcacgtcaag atgggcacgg taaactacgc gccttccgat ccgtaaaatt acgtacctgg
     1021 acgtattgta ccagaaaaag tgtcgaatgg gaaaggggga aattttttat ttttctattt
     1081 ttacaggagt ttttcctgga aagaaaaagc agccaaacgc tccttttctt tctgacgccc
     1141 cttcaccttt cgccgaaaga caagaactcc ccggcgttgc ttcttgcaac gccggggagt
     1201 tctctttaac cgcatgacgg ttgattacga acggtgttac ttcaggattc ctgcctcttt
     1261 aaagtagcgc agcgcgccgg ggtggtacgg aatgagcatc tcctcggaag gagccacgag
     1321 atcggggccg aactgccgta cagccgcggc aacggcacgg acatcccccg cgttttcgta
     1381 aagagatttg gtcatctggt agacgagatc ctcgggaacg tctatcctgg tgatgatgag
     1441 gtctgcggga tcgccgcacg tctcgacgtc gtaaaactga ttcggatagg ttcccgccgg
     1501 aagcactctc ttcgacacga aggggttgat cttcttcaga ctctgataac cggcgtcgtc
     1561 gatcggaaga ataatgacct tatcggtcac catgagctcg ctgattgccg aggcgccggg
     1621 agtgaaccca agaatcgcgc cctgaatcag gccatcctgc atcatgtcga ccgcctggct
     1681 cgcctcgacg tactcgggcg tcatatcgtc ataggtcagg ccgtaggccg ccagaatctc
     1741 gcgcgcatca agctccccgc cgctgccgac cggaccgacg gcaacacgct tgccccggaa
     1801 gtccgcaatc gaagtaatcc cggaatcctt cagcgcgagc acctgcttcg catcgaacat
     1861 cacgccgccg gtgagcgccc gaagccacgt atggggctcc ttgtacttct cgatcccgtt
     1921 atacgcacgg tacgcctcgt tggccctgca caccgcgacc tcgatgtcct cggttctgat
     1981 catgtcgatg ttctgaaccg caccggctgt ggcgatagca acggctttca tgtccttgat
     2041 attggcgttc caaatcttcg ccatcgccat tcccaccggg tagttcattc cgccgacgcc
     2101 tgcggtcccc attgtgatat gggtcacggc aagagcactc cccgtgaaaa caaaaagtga
     2161 aaccaacgct attgctgcta tgatcatttt ccggtttttc attttgtttc atcctccttg
     2221 cgggctctga gcccctgatt taggatacga ctatacaact gacactttcg tatgcgcgtt
     2281 cctgcctcat agtccggcgg cggcatcccc ctttcttcta cgaagataca atacactcaa
     2341 tattcctact gttgcaagag caaaacccgc cgcgtcggaa gaagctcccg gcgctatcag
     2401 cgtcaaagcg gctccaaaaa agataatcct gaggaaccat ggaatctcga taaagaggaa
     2461 cccctcgatg gcgacggaga gaagcgccgc tccgatgagt cccgatacgg ccgcccaggc
     2521 gatgacgaac gaactgtccg cctgcagcag aatcgcagga gcatagacga agaagaacgg
     2581 gacaatgaag ccggcgagcg caagtttgaa ggctgcccag ccgacctcgt tcgtcccggc
     2641 gcccgacatc cccgcggcaa cgtaggaggc cagggcaaca ggaggcgtta ccgtcgagag
     2701 acatgcgaag taaaagacga agaagtgcgc ctgtattccg gggacgttca actggtgaag
     2761 tatcggcacg gcgatggtcg ccgcaataat gtagcacgcg ctggtgggca atcccatacc
     2821 aagaatgatt gagacgacca tgcagagtac cagggtgatg aagaggctgc cgtgcgacag
     2881 ggcaacgata ttgtcggcaa ggatgatgcc gataccggtc atcgaaagca cggcgacaac
     2941 gaacccggcg ttcgccatcg caacactgag actcagacag gaacgagccg aagcaaccgc
     3001 catggggacg agtttgccga ctcccatgcg cgtttccttt cgcaggaacg agagcagcca
     3061 ggtgaccagg atcgagtaga atgccgcata gagcggtgtg aagtgctgca tgagcagaac
     3121 gacgaggatg atgatcggca ggaacatgtg cccgtaccgg aggagcgtct ttttcacgtc
     3181 gggcaattcc tccttggaaa gccccgtcaa accgatcttg cgcgcccgga ggtctacctg
//
LOCUS       DAOPKU010000339         3228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_33512_length_3228_cov_5.817523, whole genome shotgun sequence.
ACCESSION   DAOPKU010000339 DAOPKU010000000
VERSION     DAOPKU010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33512_length_3228_cov_5.817523"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PK849_10340"
     CDS             complement(<1..928)
                     /locus_tag="PK849_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPE66564.1"
                     /translation="MKEHLFPLFPDGRRGVSIAAKLSLMIIAGVGAIFLAIVFYGYST
                     SRAVLEEEFSARMENLGNAAESKFRQIPRVAEAVTRDLVSVLTFFNPPPGKIPEMMKV
                     LLESHPEVSGLSIGFSKQAGDPLLRNFCPIVFRRGGAILYQDLAQELDWTVQDWYQLP
                     LQMGKPLWTEPFFGDDGSGAMMVNYSVPVYDSSWRYLASVGASLELNWLADIISAMEV
                     GGRGYVFLVTSNGAFVSHPRRELLMRETLFTVAEMENRPDLIPLGHSMIGGETGVHSL
                     YDPLRGRLLVFFRPVGDMGWSLGIVFPEEEVLD"
     gene            complement(925..1941)
                     /locus_tag="PK849_10345"
     CDS             complement(925..1941)
                     /locus_tag="PK849_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66565.1"
                     /translation="MFPGSLPARRLPAFLLFVLFLLFFAGGALGQQAAPTLTKATLTL
                     QWYPQTQFAGYYMALEKGFYAARGIDLEIRRGGSDVDAIGDLRTGRTDFATLFLTAGI
                     RYRSKDLPLVNVGQIVNRGNLLIVAYKNRGIRTVRDLNGRKMSLWGDHFSGGYKKLFS
                     DNGIWPVIFPQLISVEVFLRGGVDACCAMSYNEYIRILHAGRRPEDLVVISLREEGYD
                     FPEDGIYCMEETLLRNPRLARDMREASLEGWRYAAEHPDEAVDLILRKADEAGYIVNR
                     LLLRRMLDTILPSIFPEDSSEWTPGVLSRADYERTFDMMETVFVEMTERVPYSVFFPG
                     SGER"
     gene            complement(1955..2383)
                     /locus_tag="PK849_10350"
     CDS             complement(1955..2383)
                     /locus_tag="PK849_10350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66566.1"
                     /translation="MESTVRERTFPASLDCLGGFRAFILEENGAEERLPPKRVAHLDV
                     AIEEIVVNICSYAYETPPGKLTIRVEESPEAFAVEFLDNGVAFDPLEVDEPDVSRPLE
                     ERDAGGLGILLVRRMMDEVHYSRKGTLNSLRIVVKTPRER"
     gene            complement(2398..2748)
                     /locus_tag="PK849_10355"
     CDS             complement(2398..2748)
                     /locus_tag="PK849_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPE66567.1"
                     /translation="MFEMKITVESWGRDVSLTGRLDAGSAPDFEKEAIASVDAAGESV
                     HFVLDFTNLEYISSAGLRSILVLAKRLAPSGGKVVLCGMSGVVEEVIGVSGFDSFLPV
                     AGSRGDALRIVGAV"
BASE COUNT          722 a         1001 c          910 g          595 t
ORIGIN      
        1 cgtccagcac ctcttcctcg ggaaagacga tgccgaggga ccaccccatg tcccccacgg
       61 ggcggaagaa aaccagcagc cttccgcgga gggggtcgta aagggagtgc acgccggtct
      121 ccccgcctat catgctgtgc cccaggggta tcaggtcggg gcggttctcc atctcggcta
      181 cggtaaagag ggtctcccgc atcagcagct cccgccgcgg gtgggatacg aaagcgccgt
      241 tcgaggtcac caggaagacg tagccgcggc cgcccacctc catcgccgag atgatatcgg
      301 ccagccagtt cagttccagc gaagcgccga ccgaggccag gtagcgccat gacgaatcgt
      361 agaccggaac ggagtagttg accatcatcg ctccggaccc gtcgtcgccg aagaagggct
      421 ccgtccagag aggctttccc atctggaggg ggagctggta ccagtcctgc accgtccagt
      481 cgagttcctg cgcgagatcc tggtagagaa ttgccccccc tctccggaag acgatagggc
      541 agaagttccg cagcagcgga tcccccgcct gcttcgagaa acctatcgag agtccggaga
      601 cctcgggatg agactccaga agcaccttca tcatttcggg aatcttcccg ggaggcggat
      661 tgaaaaaggt cagcacggag acaagatccc gcgtcaccgc ctcggccacg cgagggatct
      721 ggcgaaactt gctctcagcg gcgttgccga ggttctccat ccgtgcgctg aactcctcct
      781 ccaggaccgc cctcgatgtc gagtagccgt agaagacgat cgcgagaaag atcgcgccga
      841 cgcccgcgat gatcatcagg gaaagcttgg ccgcgatgga gacccccctg cggccgtcgg
      901 gaaagagggg aaaaagatgc tccttcatcg ctcgccgctc cccggaaaga aaacgctata
      961 gggaacccgc tccgtcatct cgacgaagac agtctccatc atgtcgaagg tgcgctcata
     1021 gtctgccctg gagagcaccc cgggagtcca ctccgaggag tcctccggaa agatcgacgg
     1081 aaggatcgtg tcgagcatcc ggcgcagcag aagacggttc acgatgtacc ctgcctcatc
     1141 ggccttgcgg aggatcaggt cgacggcctc gtcgggatgt tcggccgcgt agcgccaccc
     1201 ctccagcgat gcctcgcgca tatcccgggc aaggcgcgga ttcctgagca atgtctcttc
     1261 catgcagtaa ataccgtctt ccggaaagtc gtacccctcc tcccggagag agatcaccac
     1321 gagatcttcg gggcgccgtc cggcatggag aatgcggata tactcgttgt agctcatggc
     1381 gcagcaggcg tccactcccc cgcgcaggaa gacctccacc gatatgagct gggggaagat
     1441 caccggccag attccgttgt cgctgaagag tttcttgtat ccgcctgaaa aatggtctcc
     1501 ccacaggctc atcttgcgcc cgttcagatc cctgaccgtg cggatgccgc ggtttttgta
     1561 cgccacgata agcagatttc ccctgttgac gatctgcccg acgttcacca ggggcagatc
     1621 cttcgatcga taccggatgc ccgcagtgag gaagagcgtc gcgaagtcgg tccttccggt
     1681 gcggagatcc ccaatggcat cgacgtcgga gcctccgcgc cggatttcga ggtcgatgcc
     1741 gcgggcagca taaaacccct tctccagcgc catgtaatac cccgcgaact gtgtctgggg
     1801 ataccactga agggtcaggg tcgccttcgt cagggtggga gccgcttgct gccccagcgc
     1861 tcctccggcg aagaagagga ggaaaaggac aaaaagaagg aaagccggca ggcgacgggc
     1921 gggcaaagag ccggggaaca cctgaaacgc ctccctacct ttcccggggc gtcttcacca
     1981 cgatacggag actgttcagc gtccccttcc gggagtagtg gacctcgtcc atcatcctcc
     2041 ggaccaggag gatgcccagg ccgccggcat cgcgctcttc aagcggacgg gaaacatccg
     2101 gctcgtccac ctcgagggga tcgaacgcca cgccgttgtc cagaaattcg acggcgaaag
     2161 cctcgggaga ctcttccacc ctgatggtga gttttccggg aggagtctcg taggcgtagg
     2221 aacagatgtt caccacgatc tcctcaatcg cgacatccag gtgcgcaacg cgcttcgggg
     2281 gaagacgctc ctctgctccg ttttcctcca ggatgaaggc ccggaagccg cccaggcaat
     2341 ccagagaggc tggaaaagtc cgttccctga cagtggattc cattcacatc tccccctcta
     2401 caccgctccg acgattcgga gggcatcccc gcggctcccg gccacgggaa gaaaactgtc
     2461 gaagcctgag acaccgatga cttcttcaac gacaccggac atgccgcaga gaaccacctt
     2521 gccgccggaa ggagcaaggc gcttcgcaag gacgagaatg gagcgcagac ctgcactgct
     2581 gatatattcg agattcgtga agtcgaggac aaagtggaca ctctccccgg ccgcatcgac
     2641 agaagctatg gcttcctttt caaaatcggg cgctgatccg gcgtccaggc gccctgtgag
     2701 tgagacatcc cgcccccagg actcgaccgt aattttcatt tcgaacatcg ttcattcccc
     2761 cagggagagc ggccgaagcc gctaatattg acgctaaaat ccatatgcgg ccattgtgag
     2821 tgttttcgcc ggacgtgtca agaaaacttc agggagagac catttcggag cagttccaac
     2881 gaaatttcct tctccctctc tataaccggc ttctcccggc ctgtcccgat gttcccgtcc
     2941 gttttgttga ttggaaggca aatcactcct tttaggaata cattctgcct gtaaacaaaa
     3001 gcagatattg atcaggttat ccggagcagg cagcggaaaa caaaaactca tcaaaaccgc
     3061 ccaaagacta aaaattccga ttttttacca tcgtaaaata ttcacgcccg ccccttgtca
     3121 aatgcctgat ttatggtaat atactccccg cacaacccag agcaaaaaaa gcaccgaagt
     3181 tggggaatcg tccaatggca ggacgcctga ctctggatca ggtaatct
//
LOCUS       DAOPKU010000340         3212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_33721_length_3212_cov_3.655052, whole genome shotgun sequence.
ACCESSION   DAOPKU010000340 DAOPKU010000000
VERSION     DAOPKU010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33721_length_3212_cov_3.655052"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..513)
                     /locus_tag="PK849_10360"
     CDS             complement(70..513)
                     /locus_tag="PK849_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPE66568.1"
                     /translation="MFSGKRIGILLSLALLLICSSAVLASANEKIGVIDSQKIIFQHP
                     RFEAVTKQLQQISQKKENEMKMAVDKESDQNKKAQIFNTKRNEFAQEEAKLMQPIFQE
                     AQVAVRTVARLKGVTVVIEQSAVYFGGIDITDDVIQELKKKAAGK"
     gene            complement(613..1755)
                     /gene="pseC"
                     /locus_tag="PK849_10365"
     CDS             complement(613..1755)
                     /gene="pseC"
                     /locus_tag="PK849_10365"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HPE66569.1"
                     /translation="MKTLSYGRQFIDDDDIDAVVAVLKGDWLTQGPSVKEFEERFAEY
                     VGTSYAVAFANGTAALHGAYFAAGVEAGDEVITSAMTFAATGNAALYLGGTPVFADID
                     PSTLCLDPKAASKKVSKRTKVIAPVSYAGYPVDIAPFLESAGSCGAVVVEDACHALGA
                     ERNGVRVGKEAHMTAFSFHPVKHITTGEGGMVATDSEEFARRLRLFRTHGIEKEPRFM
                     TAPPDGPWATEMQILGFNYRLSELHAALGLSQLRKLDRFVLRRKEIAALYDELFEGDR
                     VLCTPPRCEGHAYHLYPLQFSAGNRAAVFEELGKRGIRGMVHYLPVPLHPYYRERFGY
                     VPGEFPHAEELYAREISLPVYYGLTDDDIRRVAEEVRRIADSFQNR"
     gene            complement(1761..2243)
                     /gene="tadA"
                     /locus_tag="PK849_10370"
     CDS             complement(1761..2243)
                     /gene="tadA"
                     /locus_tag="PK849_10370"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPE66570.1"
                     /translation="MRLQDEYFMRRALAEAASAAARGDVPVGALVVTGERLVSTGSNR
                     KENDPTAHAEILAMRCAAVSLETWNLGGCTLYVTVEPCPMCAGAIVLARISRLVFGCF
                     DPRAGACGTLYDIVRDGRLNHRCAVRSGVLEGECAVMMTEYFRNRRAVRRKNAVHERS
                     "
     gene            complement(2270..>3212)
                     /locus_tag="PK849_10375"
     CDS             complement(2270..>3212)
                     /locus_tag="PK849_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPE66571.1"
                     /translation="KVGLEAESVLNCGGSCVVDPFGEKVASLGSAEEGILFVDVDLDA
                     APRSLPVRAPEKYGAIAQKKENTRAFQSLSGPLPPLAEDELFLGVAQFSYEGTDDYLK
                     RAARMLTRSADQGASLVCLPGTPSCSGDEIAAALSSSLAGEKCMAACAVAEPGAAERR
                     LVLFDSNGLVASLHEGRYTPVETSAGRIGVVFGDEGWNPEPVRCLMLEGAEIVLWYGA
                     FEDERRRSIIEKIARTRASENRVCIVGSFSGPVRESCIIPPSGAVAASALPGESQAVF
                     ALVRRSDCRWKTVTPGTDIAAGRHPEAYLPLTETRPF"
BASE COUNT          655 a         1052 c          884 g          621 t
ORIGIN      
        1 ccgcataccg gagagaagcg gctccctcga aaatcaaact ttcagactgt gctgcaaccg
       61 gcggaaaaac tactttcctg cggccttctt cttcagctcc tggatgacgt cgtcggtgat
      121 gtcgataccg ccgaaataga cggcggactg ctcgatgacc acggtgacgc ccttcaacct
      181 cgccacggtc ctcactgcga cctgagcctc ctggaagatc ggctgcatca gctttgcctc
      241 ttcctgggcg aattcgttac gcttcgtgtt gaagatctgc gcttttttat tctgatcgga
      301 ttccttgtcc acagccatct tcatctcgtt ctctttcttc tggctgatct gctgaagctg
      361 ctttgtgacg gcttcgaacc tcggatgctg gaaaatgatc ttctgagaat ctatgacgcc
      421 tatcttctca ttggcggatg caagaacagc agaggaacaa atcaacaaaa gagccaacga
      481 aagaagaata ccgatacgtt ttcctgaaaa cattaaacta cctcctcgac agtcggtaaa
      541 tatagataca aagaaaattt tatacccaaa ccaatcaaaa gtccaggcaa atttacgtaa
      601 tccctcgtcc gtctaccgat tctggaaact atcggctatc ctgcggactt cttccgcaac
      661 cctgcgaata tcgtcgtcgg taagtccgta atagacgggc aggctgatct cccgcgcata
      721 caactcttcc gcatgcggaa actccccggg gacgtaaccg aaccgctccc tgtagtacgg
      781 gtgcagcggc acaggaaggt aatgcaccat tccgcggatc ccccgtttcc ccagctcttc
      841 gaagacggcg gcgcggtttc cggcggagaa ctgaagcgga tagaggtggt aggcatgccc
      901 ctcgcatcgc ggaggcgtgc aaagaacgcg atccccttcg aaaagttcgt cgtaaagcgc
      961 cgcaatctcc ttccgccgaa ggacgaagcg gtcgagcttc cggagctggc tcaacccaag
     1021 cgcggcgtgc agttcgctca aacggtagtt gaacccgagg atctgcatct ccgtcgccca
     1081 cggaccgtcc ggcggagcgg tcatgaaacg aggctctttt tcaatcccgt gcgtcctgaa
     1141 aagacgaaga cggcgggcga actcctcgga atcggtagcg accatgcccc cctctcccgt
     1201 cgtaatgtgc ttgacggggt ggaagctgaa ggccgtcata tgcgcttcct tgccgacgcg
     1261 gaccccgttc cgctcagctc cgagcgcatg gcaggcatcc tcgacgacca cagcgccgca
     1321 cgacccggcg gattcgagaa agggcgcaat atccacgggg tagccggcat agctcaccgg
     1381 cgcgatcact ttcgtgcgct tcgacacctt cttcgaggcc gccttcgggt cgaggcagag
     1441 cgtcgaggga tcgatgtccg caaagacggg cgtgccgcca agatagagcg cggcgtttcc
     1501 cgtcgcggca aaggtcattg cactggtgat cacctcgtcc ccggcttcca ctccggcggc
     1561 gaagtatgcc ccgtggagag cggccgttcc gttcgcgaag gcgaccgcgt agctcgttcc
     1621 gacatactcc gcgaagcgct cctcgaactc cttcacggaa ggcccctggg tgagccagtc
     1681 tcccttgaga acggccacca ctgcatcgat atcatcatcg tcaatgaact ggcggccgta
     1741 cgacagcgtt ttcattcaca tcagctcctc tcatgaacag cgttttttct tcggacggct
     1801 cttcggttcc tgaaatactc cgtcatcatc acggcgcact ctccctcgag aactccggag
     1861 cggactgcgc atctgtggtt taagcggccg tcccggacaa tatcgtagag agtgccgcac
     1921 gctccggccc ggggatcgaa acagccgaag accagcctgg aaattctcgc caggacgatg
     1981 gcgcccgcgc acatgggaca cggctccacc gtgacgtaca acgtacagcc gccgagattc
     2041 cacgtctcca gtgaaactgc cgcgcagcgc atggccagga tctccgcgtg cgcagtcgga
     2101 tcgttttcct tgcggttgct ccccgtcgag acgagccgct ccccggtaac gaccagcgcc
     2161 cccaccggaa cgtctcccct cgccgccgcg gaagcggcct ctgcgagcgc gcgccgcatg
     2221 aagtactcgt cctgtaaccg cacggcagcc tccggggtcg gcatacttct cagaaagggc
     2281 gcgtttccgt caacggaagg tacgcctcgg gatgcctccc ggcggcgatg tccgttcccg
     2341 gcgtcacggt cttccacctg cagtcggagc gtctgaccag agcgaagact gcctggctct
     2401 ctcccggaag cgccgacgcg gcgacggctc cggacggcgg gatgatacag ctctcacgga
     2461 ccggtccaga aaaggaaccg acgatgcaca ccctgttttc ggaagcccgt gtacgggcga
     2521 tcttctcgat gatcgagcgt ctgcgttcat cctcgaacgc tccgtaccag aggacgattt
     2581 ccgccccttc cagcatcagg cagcgaacag gttccgggtt ccacccctcg tcgccgaaga
     2641 cgacgcctat ccttcctgcg gacgtttcca cgggcgtata ccgcccctcg tgaagcgagg
     2701 cgactaggcc gttgctgtcg aacagcacaa gccgccgttc agctgccccc ggctctgcaa
     2761 ctgcgcaggc cgccatgcat ttttctccgg caagcgagga ggaaagcgcg gcggcgatct
     2821 catcacccga acaggaaggc gttccgggaa gacagaccag cgacgctccc tggtcggcgc
     2881 ttcgcgtcag catacgcgca gcgcgcttca ggtagtcgtc cgttccctca tacgagaact
     2941 gcgcaacccc gagaaagagc tcgtcctccg caagcggcgg aagaggcccg gaaaggctct
     3001 gaaacgccct cgtattctcc ttcttttgcg caatcgcgcc gtacttttcc ggcgcgcgta
     3061 ccggcagcga acgcggcgcg gcgtccagat cgacgtcgac gaacaggatc ccctcttcgg
     3121 cagaccccag cgaagcgacc ttctcgccga aggggtccac gacgcagctg ccgccgcagt
     3181 tcaggaccga ttcagcctct aaaccgacct tg
//
LOCUS       DAOPKU010000341         3210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_33750_length_3210_cov_4.908082, whole genome shotgun sequence.
ACCESSION   DAOPKU010000341 DAOPKU010000000
VERSION     DAOPKU010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33750_length_3210_cov_4.908082"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..604
                     /locus_tag="PK849_10380"
     CDS             257..604
                     /locus_tag="PK849_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66572.1"
                     /translation="MRGQEKPIFDFLRLLLNQRKPERRFLRLILPQMTVKNLFLPMIA
                     LCRLLNSKEKPMPDSFLPILFHQVLPVPQTYRIKPSNLPMLLFCGYRVKKTQQKPWEK
                     RDQPKPSFRREAV"
     gene            1303..1656
                     /locus_tag="PK849_10385"
     CDS             1303..1656
                     /locus_tag="PK849_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66573.1"
                     /translation="MVLLPGGNAVLSGVLPGKEYLFEALRINPTGKDVYLNISLSYLM
                     MQNKGTSSPSFSAAPARSENENALYLALLAHALREKKADEQSEQFLNAAAARASGEVL
                     RLVRSLEKKTSPETK"
     gene            1895..2977
                     /locus_tag="PK849_10390"
     CDS             1895..2977
                     /locus_tag="PK849_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HPE66574.1"
                     /translation="MNATKVDELGKKYMRQYSLYLEFAARACNLLENLVEQDGIQLYN
                     VSGEAKTPEGFLRMYEALAEGVPSSLSEIADLCTVKILVHFPEDVEKVERIVQQEFLV
                     DMARSVTSASLDDPDRFGYPAVYYTLSLSRARAAMREWSKYKDIPFSLEIRTVLQEVW
                     AANTPKVMLPTDTSTKKKIQRKLFRVGALLEEADEGYFSLWESARESAMPVTPPVARV
                     EPVASTRRVFDRNALRQYFQEHEEIPRRWAEIASDVGFPPFRPDPEYLETSLSYLWDI
                     LRASEMDSPDEFQRFIASLEEEEKGREQVATVYKAFEREAQSWRVDGYSALFLLVLNL
                     KWDVLQNKDLVELNIKRGSDRIKGIL"
BASE COUNT          783 a          770 c          844 g          813 t
ORIGIN      
        1 gccttggaag tagatgctac cagagatgac ttcgctgagg ctgtcgaaag gaccgaaaaa
       61 aaggttctta ttgcggatga ggagcgccgt cagcggaccg ctctgtcgga agagcaaatt
      121 cgacttggag ccgcttttct tgtaaccgga gaatttctgg aggcggaagc gcattttctt
      181 cgtgctctcg atcttattcc ggagggagaa gatgcgtttt tagggcttgc ggaagccagg
      241 atagggaagg gcgatattga gggggcaaga gaaacctatc ttcgacttct tgaggttact
      301 cctcaatcag cgaaagccag agcggcgctt cttgcgcttg atccttccgc aaatgacagt
      361 aaagaacctg ttcttaccga tgatcgctct atgccgattg ctgaacagca aggaaaaacc
      421 gatgccggac agctttctcc cgattttgtt tcatcaagta ttaccagttc ctcagacgta
      481 tcgaataaaa ccgagcaatc tgccgatgtt gctgttctgc ggataccggg tgaaaaaaac
      541 acagcagaag ccctgggaga agagggatca acccaagccg tcattccgcc gggaagcggt
      601 gtgacgccgc cccccgtttc agcagatatt gagatcgctt ctctcccttc ctccggcgac
      661 gttcttccgg cgaagccgtc tcctgtcgtc gagataacgg aaaagaacga ttttcctcag
      721 gcgcttgttc ttactcctga gactacgcct ctgaaggaga cgccgctttc aagcgacgtc
      781 tcctcggtga taggggaagt cgtttccaaa gagccgaagg agcgcccttc aaagagaact
      841 gaaacgccga gggcgccgtt caacggacga gtggtcaaat cgacggtatc gccgccatta
      901 tctgttccgc gacgttcccg gaagagttct gcgccaattg aaaccgggca aaatactcgt
      961 tctatagcgg cgtttaagaa gaatgaagag agaattcttg aggcaagccg ttcgtacaat
     1021 ctctccggac agtatttgaa agcggcggaa aaggcgtccg aagctcttga cttcgagcaa
     1081 tctccggagg ctctctcgaa tctgggatac gctcatatcg gtctgggcaa ttacccggcg
     1141 gcttttaccg cctactggag acggttgctg ctttctccaa agttgcggaa ggtttctctt
     1201 gaacctcccg cattcctctc ccttccgttc aaagaagggc gctggaaaga gattcctgtt
     1261 ccgaatccaa gagatctgcc tgttgttgtt cgcagcgaaa aaatggtgct tctgccgggt
     1321 gggaacgcag tcctttccgg agttcttcca ggcaaggagt atcttttcga agcgcttcgg
     1381 ataaatccaa cagggaaaga cgtatatctt aatatttcat tgagctatct tatgatgcag
     1441 aacaagggaa cttcttctcc gtcattttct gccgctccgg ccagaagtga gaatgaaaac
     1501 gcgctctatc ttgccttgct tgcccacgcg ctccgtgaaa aaaaagcgga tgagcagtcg
     1561 gaacagtttc ttaatgctgc agcggcaaga gcatcgggag aggtattgcg cttggttcgt
     1621 tccctggaga agaaaacttc tcctgaaaca aagtaaaatg cagatgtacg cttgaacgct
     1681 tttctttcac cagacgcggt tcaaatcgcg cttgaattcg aggattgagg aaaagctttt
     1741 caataaagct gatttactat atacttgcct gtggactgtc tgttccgtac tcgccctatg
     1801 agggcttgct gcaagcggcc ccgtactggc ttctgtggta aatggaaaat cgtgcagact
     1861 ctagcacttt atttttatgg gcggtgatta atcgatgaat gcaacgaaag ttgacgaatt
     1921 gggaaagaag tacatgcgac agtactctct ttacctcgag tttgccgcga gggcctgcaa
     1981 tctattggag aaccttgtgg agcaggatgg cattcaactg tataacgtgt ctggagaggc
     2041 aaaaaccccg gagggtttcc ttcggatgta tgaggccctc gccgagggag ttccctcatc
     2101 tctttcagaa attgccgatc tctgcaccgt caagattctt gtccatttcc cagaggatgt
     2161 agaaaaagtg gagcgaattg tacaacaaga attccttgtg gatatggcac ggtccgttac
     2221 atcggcaagc ttggatgatc ccgatcgttt cggttatcct gctgtgtatt atacgctctc
     2281 cctaagccgc gcacgggcag caatgcgaga gtggagcaag tataaggaca ttccgttttc
     2341 tttggagata cgaacggttc ttcaggaggt ctgggctgcg aatacaccga aggtcatgct
     2401 tccgacggac acgagcacaa aaaagaaaat tcaaagaaaa ctctttcggg taggcgctct
     2461 tctggaagaa gctgacgaag ggtatttttc tctttgggaa tctgcccgag aatctgcaat
     2521 gcccgtcact cctcccgttg cccgggttga gcctgtcgca tcaacccgac gggttttcga
     2581 cagaaacgca ttgagacagt atttccaaga acacgaagag attccccgcc gatgggcgga
     2641 gatagcttct gatgtcggat tccctccgtt caggcccgat cccgaatatc tggagaccag
     2701 tctctcttat ctttgggata ttcttcgtgc ttccgaaatg gattctcccg atgaatttca
     2761 gcggttcatt gcctccctcg aagaggaaga aaaggggaga gagcaagttg ctacagtgta
     2821 taaagcattc gaaagagaag cgcagtcgtg gagggttgac ggctactctg cccttttcct
     2881 gttggtgctg aatctaaagt gggatgtttt gcagaacaag gatcttgtgg agctcaatat
     2941 caagagaggt tccgacagga tcaaaggaat tctctaggga gttagatatt tttcccgctt
     3001 ttcggatggg gagtagcggc gcgctctccc cattgttttt ggcttgccag aaaggaagag
     3061 cgttatggat acaatagctc gactgcccgg gagaggtcaa aatgctgttt tttgcggagg
     3121 aaatcaaacg agttttcgag tactgcaaat agctccgcag ctcacttttc cggaactctc
     3181 gttccccgtt tcttcgtctt ctcccattcc
//
LOCUS       DAOPKU010000342         3202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_33873_length_3202_cov_4.599301, whole genome shotgun sequence.
ACCESSION   DAOPKU010000342 DAOPKU010000000
VERSION     DAOPKU010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33873_length_3202_cov_4.599301"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..544)
                     /locus_tag="PK849_10395"
     CDS             complement(242..544)
                     /locus_tag="PK849_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66575.1"
                     /translation="MDEKTIYKQKIEARLALAHAQHKLNDLKTLAQNCPEDKVLEFSK
                     GIEELEKNFIHAKLKFREMNEAGDEAWRNFKESAEQIWNALRVSVKNATARYKELH"
     gene            1126..1431
                     /locus_tag="PK849_10400"
     CDS             1126..1431
                     /locus_tag="PK849_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66576.1"
                     /translation="MDDKRAQRMISDEDRTALRLLQHFCYTIGSANDAEDHGYGGEAR
                     RMREESCESIRNLADQHPLLTEFFPGLKEELETGRFLAFGWSSIAREADELLAGGAL"
     gene            1428..1748
                     /locus_tag="PK849_10405"
     CDS             1428..1748
                     /locus_tag="PK849_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66577.1"
                     /translation="MNIPGEDRTQCDVCSSLSESEYGYSKYGWPDHDVDLPDAAGSLV
                     MVKDLKPLSERKLQLWRCPGCGAWFLYTTDYEYLTNGTEDEQFLTRLSEEEAAEYLNR
                     PEAP"
     gene            complement(1868..2164)
                     /locus_tag="PK849_10410"
     CDS             complement(1868..2164)
                     /locus_tag="PK849_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66578.1"
                     /translation="MYALTKEAYRRKIQARLGLAHAQAKLNELKEKNKTSSANPVGEY
                     SNEINELEKNIEETQKRLNELYEAGDEAWEHLDDTSERILNALRTAVKNAVAKQ"
     gene            complement(2293..2514)
                     /locus_tag="PK849_10415"
     CDS             complement(2293..2514)
                     /locus_tag="PK849_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66579.1"
                     /translation="MDIVKIIAIGLIATGALGLTYGGFTYTQTTHVAGLGPIRFSVDG
                     NRTVYIPLWASGTAVALGGILLLFGGKLI"
BASE COUNT          727 a          779 c          818 g          878 t
ORIGIN      
        1 caatgcagcc taaggcgtcc ggaacggtta cgacttaggc tgcatcaggt tctgcacgtt
       61 tcatttatga ttgaggatca tcgatgcgtc tacggacccg ttccctccaa gaagcgcggg
      121 aacataggag caggggcgca tcgttgatgc cgttcttaaa aacgaacggg aagcgcccca
      181 aggagagtcg ttaaaaaaca acgaccgaca ctctcttgcg tgcgtcggtc gttgttctct
      241 tttaatgtaa ctccttgtat ctggcagttg catttttgac ggatactctc aatgcgttcc
      301 agatttgctc cgcgctctcc ttgaaatttc tccacgcctc atcgccggct tcattcatct
      361 ccctaaattt gagcttcgcg tggatgaaat tcttttcaag ctcttcgatc cccttgctga
      421 attctaggac cttgtcttcc ggacaatttt gagccagggt cttcaaatcg ttcaatttgt
      481 gttgcgcgtg cgccaacgcc aagcgcgctt ctatcttctg tttgtagatt gttttttcgt
      541 ccacaggcgt cacctcctta tagtagatac agtatttgtt gttatgaaaa tttttcgtgc
      601 gcgctgcgag catttccatg aggcgacttc caatgaaaat acgcgccatt tgccaaatat
      661 attgaaattc ttaatgaata gaaacatata catcgaattc aattatacgg taaattttaa
      721 aaatcacaag agggggtttg tatttttaca gtataaggaa tctctgaata aaagaatttt
      781 tcgcctcata cggctcgata gagtctcttt tcacaagacg tggaacagtt tttttgatat
      841 cccttaagag ttctttctat tattgccggc ttgatgttga tcgaagcacg aagcgccccg
      901 agttttcaat agcagttccg tatggagcgc ttctgcgttt ttgtctccga aggtatgcta
      961 tgcatctttt ccggccgggg agtataatcc gtaagaggac agtgtgattt caatggcttt
     1021 taagctgttt ggaacgcgat tttcagggga gggataggtt gtgacacgtt ctttctgttc
     1081 ggccgttttt ccgcttcatc cgttcttcac tgcgggggtg gtgtgatgga tgacaagaga
     1141 gcgcagcgaa tgatcagcga cgaggaccgt accgcgcttc gcctgctgca gcacttctgt
     1201 tatacgatcg gcagcgcgaa cgacgcggag gatcacggat acggcggaga ggcgaggcga
     1261 atgcgggagg aatcctgcga gtcgatccgg aacctggcag accagcaccc tctcctcacg
     1321 gagttctttc ccggactgaa agaggagctg gaaaccggac gttttctcgc gttcggatgg
     1381 agctcgatcg cacgcgaagc ggacgagctg ctcgccggtg gcgccctatg aatatccccg
     1441 gggaagaccg cacgcaatgc gacgtctgtt cgagtctttc ggagagcgag tacgggtaca
     1501 gcaaatacgg ctggccggac cacgacgtcg atctgccgga tgccgcaggt tctctcgtta
     1561 tggtgaagga tctcaagccc ttgagcgagc ggaagctgca actctggcgg tgtccagggt
     1621 gcggtgcatg gtttttgtac acaaccgatt acgagtactt gacgaacgga acggaggacg
     1681 agcagttcct gacccggttg agcgaggagg aagccgccga atacctgaac cggccggaag
     1741 ctccgtagtc ctgacggttg ctgttatcgc atcctcgttg cctttgcagg ctgtcggtta
     1801 cagacaacgg ttttttaaac ctcgttaaac gccctgaacg ggtgattatc gaggcttcaa
     1861 agtatcctta ttgttttgcg acggcatttt tcacggcggt ccgtaacgcg ttcaatatcc
     1921 gctccgatgt gtcgtcgaga tgctcccagg cctcgtctcc ggcttcatac agctcgttca
     1981 ggcgtttttg cgtttcttct atattctttt caagttcgtt gatttcgttg ctgtattctc
     2041 caaccggatt cgctgaagaa gtcttgtttt tctccttcag ttcattgagt ttggcctgcg
     2101 cgtgcgccag ccccaatcga gcctgtatct tacgtctgta ggcttccttt gtaagtgcat
     2161 acactacgcc atctcctctt gttgcctccc aagttctttc cggtttttcc cggcgcgcat
     2221 gaaaaacaac gcccgtgacc gggttccaat ctctttgctg aatgcaagag cggcatctgc
     2281 cggaaagaca gtttatatca gttttcctcc gaagagcaga agaattccgc cgagagcgac
     2341 cgcggtcccg ctcgcccaaa gcggaatata gacggtccga ttcccgtcca cggagaatcg
     2401 gatcggcccc agtccagcca catgggttgt ctgcgtatag gtgaatcctc cataggtcag
     2461 ccccagtgcg ccggttgcga tcagccctat tgcaatgatt ttcactatgt ccattcgact
     2521 ccctcctccg ggaaaaatgc ttcgatttcg ctgtgaacgc cgaaagaatt gcattgtcgt
     2581 ccgtgtgaaa atcgggaagc cggcgagcga cgttgttgcg ggacaacccg acgtgatttc
     2641 attgcctgtc ggcgagttga tcgcttcgtc cttccgctca caacgaaatt attatatatc
     2701 attttgaaaa aacagtcaag tgaaaattta atattatatt tgcattttac gcctatttat
     2761 tattggttta ttcagtataa agtgaatttt ttcaggctgt tttcagggct gtatgctgta
     2821 ttatgagacg cctcgctctc agcgcgtttt tggtcgtcga acgagcgtat cggattttgg
     2881 tattgtaaaa atcaaaaaac aggggtataa tgcccaataa gcggatttta aaatccgcct
     2941 gtcggaataa agatgctgct ttacggataa aaagaagaaa aatttcatca ggtggaacct
     3001 tgtcgaaggg aaagcagagg agtgtagagc gtgagaataa tggaccaaca gggagaaaat
     3061 gctgcggaca agcagggaat ccgcgttctt gaacgcctct tcgacattct ctccttcctt
     3121 gccgaacggc acgactacgc ggggataaag gagatcgccg aagcgacggg tctgtcgcgg
     3181 acaacggtac atcgcgttct gg
//
LOCUS       DAOPKU010000343         3201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_33882_length_3201_cov_4.431977, whole genome shotgun sequence.
ACCESSION   DAOPKU010000343 DAOPKU010000000
VERSION     DAOPKU010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33882_length_3201_cov_4.431977"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PK849_10420"
     CDS             complement(<1..415)
                     /locus_tag="PK849_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE66580.1"
                     /translation="MNIYPESAPGKGSLRCIAALLLLLLAVSIAEGAGEARTLALEEC
                     IRLAEEHHPSLEEARASLNAQKARLDAVKAGNALTGSLSASTGRQTGSDQSYSTSFTV
                     TKLLSDSGKNALERRSQGLAIDTASESQREAVLTVR"
     gene            657..2303
                     /locus_tag="PK849_10425"
     CDS             657..2303
                     /locus_tag="PK849_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPE66581.1"
                     /translation="MSETMNSDMQAPAAGGRSTTIRKGDVLLQLEGGGVWLACRGDSV
                     PFPADVEAALEEGGVTERDEDALRKFLDGKDRRVLIASAFVPVDAKVKVAMAKDSMSV
                     SLLVSPPSLGGAMPSPDELEEQLHRHGAVYGIDKSALAKLCAEKLFDEYVEVARGTPA
                     VHGSDAAVEVVVGTGGRRGPVEDEDGNVDLKNLGIVNLVHAGDVLATKTPPTEGVEGH
                     TVRGGVLGAKNGKDKSFPAGPGTVVSEDGLSLLAAVEGHLTEVNGRLQVLPVFEVNGD
                     VDYSTGNIDFPGSVVVKGVVRDGFEVRASENITVNGTVEGAFLVAGGNITVVGGIRGV
                     SKGKVEAKGNIAADFADQATLFADGDILIKNALLHSDCRCGGKLVVSGGKKSQIAGGK
                     IQAGSEVVCATLGSEMGTKTEIAVGALPEQVERRDFLRKELHQTTENLEKVEKNIAFL
                     KHAEAEKGLDDEKRAMLGKLTRTAFSCKASMKQLGDEAALLEADMERKRERGAVRVRG
                     TCYPGISVSIRGLTYRVREEFKFGALLYDEGEIKVKPFDA"
     gene            complement(2366..2539)
                     /locus_tag="PK849_10430"
     CDS             complement(2366..2539)
                     /locus_tag="PK849_10430"
                     /inference="COORDINATES: protein motif:HMM:NF025436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HPE66582.1"
                     /translation="MDMGMVNNVMATNQSNTMAQVSTSVQKKAMDMMELQGQMLHEMM
                     ASMGIGQNLDIQG"
     gene            complement(2678..>3201)
                     /locus_tag="PK849_10435"
     CDS             complement(2678..>3201)
                     /locus_tag="PK849_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66583.1"
                     /translation="YPHELDGGRRQRVGIARALSLSPDFIVCDEPVSALDVSIQAQIL
                     NLLQELQEKMKLTYLFVSHDLSVVKHISNRIAVMYLGKIVESAPSLELFKNTLHPYSQ
                     ALLAAVPIPKLDRKREKFILTGGVPSPINPPPGCRFHTRCPWKMDVCSAEEPPLIDVG
                     GEHLVACHKVKGS"
BASE COUNT          589 a          921 c         1068 g          623 t
ORIGIN      
        1 tccggacggt caggacagcc tcccgctgcg actcggaggc agtgtcgatg gcgagcccct
       61 gactccgccg ttcgagcgcg ttcttccccg agtcggagag gagcttcgtc accgtaaagg
      121 aggtcgagta gctctggtct gatcccgtct gccgtccggt ggaggccgaa aggcttcctg
      181 tgagtgcgtt tccggccttt acagcgtcga gccttgcctt ctgcgcgttc agcgacgccc
      241 gcgcctcctc cagcgacgga tgatgctcct ccgcaaggcg aatgcactct tcgagagcga
      301 gcgttcgcgc ctcgccggcc ccctccgcga tgcttaccgc gagcaggagc agcagcagcg
      361 ccgcaataca gcggaggctc cctttccccg gtgcgctttc agggtatatg ttcattgatc
      421 tccatcccct ttttctgtgt tttcatgcct ctattgtata gctctttcat tcagcgtgat
      481 ctgtagttgg cgcaaagatt tgtaagggaa agaaccgaag tgtaaagccg gcggtgcagt
      541 gtacccccgt tgagtgtgcg cgttcgttgc cgttttcgat ggaattggtg tatcattgat
      601 aaaaatacgg tgttttccgc cgtcccccgg gcggcgtaca ggaaagggag atgcgtatga
      661 gcgagacgat gaacagcgat atgcaggccc ccgccgcagg cggacggtcg acgacgatcc
      721 ggaagggaga cgtcctcctg cagctcgaag gagggggcgt ctggctcgcc tgtcggggcg
      781 attccgttcc ctttcctgcg gacgtcgagg ccgccctgga ggagggcggt gtgactgaaa
      841 gagatgaaga tgctctccgg aagttcctcg atggaaagga ccgccgggtg ctcatcgcct
      901 ccgcgttcgt tcccgtcgac gcgaaggtga aggtcgccat ggcgaaggac tccatgtcgg
      961 tctcgcttct tgtttctccc ccatctctcg gcggcgccat gccttcgccc gatgagctgg
     1021 aggagcagct tcacaggcac ggcgcggtgt acgggatcga caaaagcgcg ctggcgaagc
     1081 tgtgcgccga aaagctcttc gacgagtatg tcgaggtcgc ccgcggaacg cccgcagttc
     1141 acgggtcgga tgcggccgtg gaggtcgtcg tcggaaccgg agggcgcagg gggccggtcg
     1201 aggacgaaga tggcaatgtg gatcttaaaa atttgggcat cgtcaacctg gtgcatgccg
     1261 gggacgtgct tgcaacgaag acgccgccga cggagggcgt ggaggggcat accgtccggg
     1321 gcggcgttct cggagccaag aacggcaagg acaagtcctt tccggccggg cccggaacgg
     1381 ttgtttccga ggacgggctt tcgctgctcg ccgccgtcga ggggcatctt acggaggtca
     1441 acggacggct ccaggtgctt ccggtttttg aagtgaacgg cgacgtcgac tacagcacgg
     1501 ggaacatcga ctttcccgga tcggtcgtgg tgaagggggt cgtccgggac ggtttcgaag
     1561 ttcgcgcttc ggagaacatc acggtgaacg gcaccgtcga gggcgcgttt ctggtcgccg
     1621 gaggcaatat caccgtcgtc ggcggtatcc gcggcgtcag caagggaaag gtcgaggcga
     1681 agggcaacat cgcggcggac ttcgccgacc aggcgacgct cttcgcggac ggcgacatcc
     1741 tgatcaagaa cgcgctgctt cacagcgact gccggtgcgg aggaaagctc gtcgtctcgg
     1801 ggggaaaaaa gtcgcagatc gccggaggaa agatacaggc cggaagcgag gtcgtctgcg
     1861 ccacgctcgg cagcgaaatg ggcacgaaga cggagatcgc ggtcggcgcg ctgccggaac
     1921 aggtggaacg gcgggatttc ctgaggaagg aactgcacca gacgacggaa aatcttgaga
     1981 aggtggagaa gaacatcgcc ttcctgaagc atgcggaggc cgaaaaggga ctggacgacg
     2041 aaaagcgcgc gatgctgggc aagctcaccc ggaccgcctt ttcgtgtaag gcgtcgatga
     2101 agcagctggg ggacgaggcc gcgctgctcg aggccgatat ggagcgaaag cgcgaacgcg
     2161 gcgcggtgcg ggtcagaggt acctgctacc ccggcatctc cgtgtcgatc cgcggactca
     2221 cctaccgggt gcgggaggag ttcaagttcg gcgcgcttct gtacgacgaa ggggagatca
     2281 aggtgaaacc gttcgacgcg tagcgcggcg gaatacgttg tcacatgcga aggggggcgt
     2341 ttccgccccc cttcgttgca tcggtctagc cctggatgtc caggttctga ccgatcccca
     2401 tggaggccat catctcatga agcatctgac cctgaagttc catcatgtcc atggccttct
     2461 tctggaccga agtgctcacc tgagccatcg tgttcgactg gttggtcgcc atgacgttgt
     2521 ttaccatgcc catatccatg cgcctcacct ccttctcggg ggatgcgttt cctttatttc
     2581 tcggttgtcg cgccccgaaa gattatcctg ctgcgtgttt ccccagctgt tctcaccggt
     2641 gtaaagaagt cgcggcgagc actctgcctc tatgatatca cgagcccttc actttgtgac
     2701 aggccaccag atgttcgccg cccacgtcga tcaacggcgg ctcctcggcg gagcagacgt
     2761 ccatcttcca cggacagcgc gtgtggaagc ggcaccccgg cggcgggttg atcggactgg
     2821 ggacgccgcc cgtgaggatg aacttctccc gcttccggtc gagcttcggg atggggacgg
     2881 ctgcgaggag cgcctgcgag taggggtgga gcgtgttctt gaacagctcc agcgagggcg
     2941 ccgactcgac gatcttcccc aggtacatca cggcgatgcg gttgctgatg tgcttcacca
     3001 ccgagaggtc gtgcgacacg aagaggtagg tcagcttcat tttttcctga agctcctgga
     3061 gcaggttcag aatctgcgct tgaatggaga cgtcgagcgc cgagaccggc tcgtcgcaga
     3121 cgatgaagtc cggcgagagc gagagcgcgc gggcgatgcc gacccgctgc cttcgtccgc
     3181 cgtccagctc gtgcgggtac t
//
LOCUS       DAOPKU010000344         3184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_34134_length_3184_cov_4.689677, whole genome shotgun sequence.
ACCESSION   DAOPKU010000344 DAOPKU010000000
VERSION     DAOPKU010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34134_length_3184_cov_4.689677"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PK849_10440"
     CDS             complement(<1..653)
                     /locus_tag="PK849_10440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE66584.1"
                     /translation="MNTTDTAAVPSWRRSGSRRRIALLFLVLIPTITSSYTMARLLPE
                     RASLVLDALLVAVFGILFAWISVGFWAFMGGLFVLLTKRDRYRVSKSCDGRDLSIPDA
                     NARTAILIPIYNEEVNRVFAGVRTVYRSLKQTGALDRFDIFLLSDSTSPDVWVEEEEA
                     WHDFRREEEGTEAHVFYRKRDNNIKRKSGNVADFCRRWGKNYRYMIVFDADSVMTGE"
     gene            complement(650..1057)
                     /locus_tag="PK849_10445"
     CDS             complement(650..1057)
                     /locus_tag="PK849_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66585.1"
                     /translation="MTGLPKNIPADTHQERAAWRTSRDRVLLYLRGLDLPPFEDLDLA
                     AASLRACGAREESAAEGPGSPSPVEAMESLFNLLEEQRPEGLLGMRRIPSCPSLNRGV
                     MVAEPMDRVPWKTAFVRWMRRWRRELFAREGAR"
     gene            complement(1275..>3184)
                     /gene="mdoH"
                     /locus_tag="PK849_10450"
     CDS             complement(1275..>3184)
                     /gene="mdoH"
                     /locus_tag="PK849_10450"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HPE66586.1"
                     /translation="WTALMGFFLLLQRRNRYSVSNVCEGLGYSIDDPQARTAVLIPIY
                     NEDVKRVFAGLKTVYDSLAATGSLDRFDFFVLSDTTDPDVWVQEEEGWHRFCTEQDAF
                     GRVFYRKRRSNAKRKSGNVADFCRRWGKNYRYMIVFDADSIMAGRTMVRMVQMMEARP
                     DIGILQTPPAGVNRETLLARAQQFANRVYGPMFAAGLHFWQLGDAQYWGHNAIIRVEP
                     FIRHCELPRLPGKGPLGGDILSHDFVESALMRRAGYGVWLAYDLDGSYEETPPTLLDE
                     LKRDRRWCQGNLQHLRLIFSRGFFPAHRALFLNGILSYGSALLWLAFLLISSAQAVAE
                     VYLEPVYFLDTKTLFPQWPVWRPHWALALLGSTAVLLFLPKFFSYALALLRDPAARLY
                     GGRLRLACGILLEVALSTLLAPVRMLHHSVFILSTILGKNVGWPTQSRDDRGTPWADA
                     VDVHWWGTILGILWGALVYLTNPSFFWWISPIILSLVLSIPLSVLTSRASLGKAFLRA
                     GFFTTPEETEPPVELRELAKTLQEEAPYSPLPLSPRQGFLRAVTDPCVHALHTSLLLR
                     YKKGAPAREEARQALVEKALRSGPDSLSSKEKMTLLNDPFHLAELHRRVWELEDDKQA
                     ARWGLSQPSVD"
BASE COUNT          596 a          985 c         1051 g          552 t
ORIGIN      
        1 ctctcgccgg tcatgacgct gtcggcgtcg aagacgatca tatagcggta gttcttcccc
       61 cagcggcggc agaagtccgc cacgttgccg ctcttccgct tgatgttgtt gtcccgtttc
      121 cggtagaaga cgtgcgcctc cgtcccctcc tcctcgcggc ggaagtcgtg ccacgcctcc
      181 tcctcttcca cccagacatc tggactggtg ctgtcgctga ggaggaagat gtcgaaacgg
      241 tcgagggcgc ctgtctgttt cagcgagcgg tagacggtgc gcacccccgc gaagacgcgg
      301 tttacctctt cgttgtagat cgggatgagg atcgcggttc tcgcgttcgc gtcgggaatg
      361 gagaggtcgc gtccgtcgca gctcttcgag acgcgataac ggtcgcgctt cgtcaggaga
      421 acgaagagcc ctcccataaa ggcccagaag cccacggaga tccacgcgaa gagaatgccg
      481 aacacggcca cgagaagcgc gtcgaggacg agactcgcgc gttcggggag caggcgggcc
      541 attgtgtagc tcgacgtgat tgtgggaatg aggacgagga agagcagcgc gatgcgccgt
      601 ctgcttccgg aacgtctcca ggaggggacg gcggcggtgt cggttgtgtt catctcgcgc
      661 cctccctggc gaagagttca cggcgccagc gccgcatcca gcggacgaac gccgtcttcc
      721 acgggacgcg gtccatcggt tcggcgacca tcacgcccct gttcagagaa gggcacgagg
      781 ggattcgccg catgccgagc agcccttccg gacgctgctc ttcaagcagg ttgaagagcg
      841 actccatcgc ctcgacgggg gacggtgagc ccgggccttc cgcagcggat tcctcgcgcg
      901 ctccgcaagc gcggaggcta gcggcggcga ggtcgaggtc ttcaaaaggc ggcaggtcca
      961 gtccgcgcag atagagcagc acgcggtcgc gcgaagtccg ccacgcagcg cgctcctgat
     1021 gagtatccgc cggaatgttc tttgggagcc ccgtcatcgt gcttcctcct ccgcaaagcc
     1081 ttctgctgaa gaaagccccg cagccgcggg aaagggaaga gaacgcggct gtgttgcggc
     1141 ggcgtgtccg gtgcagtgca taaaatcctc tccttgatcg tgtgggtgta tttattgagt
     1201 atacttcctt ttggttgaag agagaaggta aaaaacagtg agaggagcaa attccgctcc
     1261 tctcacgtgc gtgttcagtc gacggacggc tgcgagagcc cccaccgggc ggcctgtttg
     1321 tcgtcctcca gctcccagac acgcctgtga agctcggcga ggtgaaaggg atcgttcagg
     1381 agcgtcattt tctcctttga ggaaagagag tcgggaccgg aacgaagcgc tttctccacg
     1441 agggcctgcc gcgcctcttc ccgcgcgggc gctccctttt tgtagcgcag cagcagcgac
     1501 gtatggagcg cgtggacgca cgggtcggtc acggcgcgga ggaatccctg ccgtggtgac
     1561 agaggaagag gtgagtacgg cgcttcttct tgaagggttt tcgccagctc gcgcagctcc
     1621 accggcggtt ccgtctcctc cggagttgtg aagaaccccg cccgcaggaa cgccttcccc
     1681 aggctcgccc tgctcgtgag gacggagagc gggatggaga gtacgagcga gaggatgatg
     1741 ggcgatatcc accagaagaa ggaggggttc gtcaggtaga cgagcgcgcc ccagaggatg
     1801 ccgagtatcg tcccccacca gtgaacgtcg acggcgtccg cccatggggt tccccggtcg
     1861 tcgcgggact gggtcggcca accgacgttt ttgcccagaa tggtcgagag aatgaagacg
     1921 ctgtgatgca gcatccgcac gggggccagc agcgtggaga gcgccacctc cagcagaatg
     1981 ccgcaggcga gccggagcct ccccccgtaa agacgcgccg ccggatcgcg cagaagcgcc
     2041 agcgcatagc tgaagaactt ggggaggaag agcagcaccg cggtcgagcc gaggagcgcg
     2101 agcgcccagt gaggcctcca gaccggccac tgcgggaaga gcgtctttgt gtcgaggaag
     2161 tagacaggct ccaagtagac ctctgccacg gcctgcgctg agctgatgag caggaaggcc
     2221 agccagagca gcgccgaacc gtacgagagg atgccgttca ggaagagcgc gcggtgcgcc
     2281 ggaaaaaagc cccgcgaaaa gatcagccgc aggtgctgca agttgccctg gcaccagcgc
     2341 cggtcgcgct tcagctcgtc gagcagggtc ggcggcgtct cttcgtagct gccgtcgagg
     2401 tcgtacgcca gccagacgcc gtacccggcg cggcgcatga gggccgactc cacgaagtcg
     2461 tggctcagaa tgtcgccgcc gagcggcccc tttccgggga ggcgcggcag ctcgcagtgc
     2521 ctgatgaacg gttctaccct gatgatcgcg ttatggcccc agtactgcgc gtcgccgagc
     2581 tgccagaagt gcagtcccgc ggcgaacatc ggcccgtaga cgcggttggc gaactgctgc
     2641 gcccgcgcga gcagcgtctc gcggttcact cctgcgggcg gcgtctgcag aatgccgata
     2701 tcgggccgcg cctccatcat ctgtaccata cggaccatcg tccgtcccgc cataatgctg
     2761 tcggcgtcga agacgatcat atagcggtag ttcttccccc agcgcctgca gaagtcggcc
     2821 acgtttccgc tcttccgctt cgcgttgctc cggcgcttcc ggtagaagac ccgcccgaag
     2881 gcgtcttgct ccgtgcaaaa acgatgccag ccctcttcct cctgtaccca gacgtccgga
     2941 tcggtagtgt cgctcaggac gaagaagtcg aaccggtcga gcgaccccgt cgcggcaagc
     3001 gaatcgtaga cggtcttcag gcccgcgaag acccgtttca cgtcctcgtt gtagatcggt
     3061 atcaggacgg ccgtccgggc ctgcgggtcg tctatagaat acccgagccc ctcgcagacg
     3121 ttggagacag agtagcggtt gcgtcgctgc agaaggagga agaatcccat cagcgcagtc
     3181 caga
//
LOCUS       DAOPKU010000345         3168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_34367_length_3168_cov_4.205589, whole genome shotgun sequence.
ACCESSION   DAOPKU010000345 DAOPKU010000000
VERSION     DAOPKU010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34367_length_3168_cov_4.205589"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PK849_10455"
     CDS             <1..442
                     /locus_tag="PK849_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE66587.1"
                     /translation="IPSRAVLTVDIRDPEGARLAEGETKLLRFLEELQTKEGVEISTE
                     SLARFEPVLFDEELASKIEEAAKKRGFSSMRITSGAGHDAQMLARVAKAAMIFVPSRG
                     GISHNPVEYTPDKQLLQGVHVLLDVAAGRLLKEGNKTEVEQKSL"
     gene            537..1790
                     /locus_tag="PK849_10460"
     CDS             537..1790
                     /locus_tag="PK849_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HPE66588.1"
                     /translation="MKVSVTSEIGRLRAVIMQPPGKGIERCTPLNVGALAWDSVPSAS
                     KAAEEHKKWVAAVEGFGAGVFLFEDLLRDVLALPSVKNELVAAVLETEKAFLTERTLD
                     ILREHLLGLSPSALVEQLFFGMTKDELHRSSGEIALRDLVDRTSEWCLFPMTNVLYSR
                     DPAAVLGEGVVFGKMFNRDRVKEPACIRAIFRHHPEFRDSEFSSWYGEEPEDTTPVEG
                     GNVHCYSRNVFVIGVNERTHPDSIEKIARRTMRCGEITDVLALLFENQRLGPADSIGL
                     TVHVDMFLNMIDHDAFLFFPYIEKKLTVLHITRGRGDRLRIEREDSLFGAIQKVLKLD
                     AVRVIKVGGEESEAVAFSEQRAGSGGNTFNLEPGRVCIWDRNVATIKALEKGGIKVVP
                     LDADELVKGGGGPRCSTMPLWRDDV"
     gene            complement(1811..2581)
                     /locus_tag="PK849_10465"
     CDS             complement(1811..2581)
                     /locus_tag="PK849_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPE66589.1"
                     /translation="MTTVLSSIKEGVCRIELNRPEAMNTFNEDLAIGLDEAFRAADAN
                     DEVRVIIVSGAGKHFSTGIDLRELLSKERHEIPGFLKLMDLHNHRLASLTKPVIASVH
                     GYALANGTGLALACDFVVASEDAVFGTTAVNVGLICLEPGYQLARWIGEKRALQYVLF
                     GELIPASKGLELGFVHTMTARETLEEKTLELAKKLASKSALSLRAGKVGFARMEGLAL
                     ERAIQAGGDIFAALAASEDGREGVEAFLAKRPPLFKGR"
     gene            complement(2594..>3168)
                     /locus_tag="PK849_10470"
     CDS             complement(2594..>3168)
                     /locus_tag="PK849_10470"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPE66590.1"
                     /translation="HTNASGGVVCAAPTGGAAGVLPGVLTTLTEDLKISRKAVLMALW
                     AAGGIGLFHDIRSTFAAEVAGCQVEIGAAGAMAAAAVVEAAGGSARQGCDAAATVFQN
                     MMGSVCDLVQGVVEIPCHSRNAALASQAFLCADLVLGGYRSPVPLDETIDAVDSTGRM
                     LPEELRCTSRGGLALCPSAQRLPRLDKKIS"
BASE COUNT          655 a          872 c          944 g          697 t
ORIGIN      
        1 gatcccatcg agggctgttc ttactgtgga tatccgcgat ccggaaggcg cccgtcttgc
       61 cgaaggggag acaaaacttc ttcgttttct ggaggaactc cagacgaagg agggggtgga
      121 aatatccacg gagagtcttg cgcgtttcga accggtgctt ttcgacgagg aactcgcgtc
      181 aaaaatcgag gaagcggcaa aaaagcgggg tttttcttcg atgcgcatca catcgggcgc
      241 cggacacgac gcccagatgc ttgcaagggt cgcaaaagcg gcgatgatct ttgtgcccag
      301 cagaggcggc ataagtcaca atcctgtgga gtacactccg gacaagcagc tccttcaggg
      361 tgttcatgtc ctgctcgacg tcgcggccgg gcgtcttctc aaggaaggaa acaagacgga
      421 ggttgagcag aagagtctgt agggcttcat gtttttcaag catctgattc cgggaaatac
      481 gaacgaacgc atgttcttat ctctgacttt gtatccaccc gcaggaggga gatagagtga
      541 aggtcagcgt tacttccgaa ataggacggc ttcgcgccgt catcatgcag ccgccgggca
      601 aggggataga acgatgcacg ccgttgaatg tcggcgctct cgcgtgggat tcagtgccga
      661 gcgcttcaaa ggcggcggaa gagcacaaga aatgggtggc ggcagtggag ggattcggcg
      721 ccggagtttt cctcttcgag gacctgttgc gcgacgtact cgcgttaccc tctgtgaaga
      781 acgaactggt tgctgctgtt ttagagactg aaaaggcctt tctcacggaa cggacgctgg
      841 atattctccg tgagcattta ctcgggcttt ctccaagcgc gcttgtggag cagctctttt
      901 tcggtatgac gaaggatgag ctgcaccgtt cttcgggaga gatcgcactg cgcgatctgg
      961 ttgaccggac aagcgagtgg tgtctttttc caatgaccaa tgtgctctat tcccgtgatc
     1021 ctgccgctgt gcttggagag ggcgttgtct ttggaaagat gttcaaccgg gacagggtga
     1081 aagagccggc atgcatcagg gctatcttcc ggcatcatcc cgagtttcgc gactcggaat
     1141 tttcctcgtg gtacggcgaa gaaccggagg acacgactcc tgtcgaggga ggaaacgtcc
     1201 actgttacag cagaaacgtc ttcgtcatcg gagtgaacga gcgcacgcac ccggattcca
     1261 tagaaaaaat cgcgcggagg acgatgcgct gcggggagat taccgatgtg ctggcgctgc
     1321 tcttcgaaaa ccagcgtctc ggccctgcgg actccatcgg cctgaccgtc catgtcgata
     1381 tgtttttgaa catgatcgac catgacgcgt ttctcttctt cccctatata gaaaaaaaac
     1441 tcactgtact tcatatcacg aggggaagag gggatcgcct gcggattgag agggaggatt
     1501 ctctgttcgg ggccattcaa aaagtgctca agctggacgc agtccgcgtt atcaaggttg
     1561 gcggcgagga gagcgaggca gtcgcctttt ccgagcagcg cgcaggttcg ggcggaaata
     1621 cgtttaacct cgaaccgggg cgtgtgtgca tctgggacag aaacgtcgcg accatcaagg
     1681 cgcttgaaaa aggcgggatc aaggtcgttc cgcttgacgc ggacgaactt gtgaaaggcg
     1741 gcggaggtcc ccgatgctcg acgatgccgc tgtggaggga cgacgtgtaa gccgtccctc
     1801 cacagggatg tcagcggcct ttgaagaggg gagggcgctt ggcgaggaag gcttcgacac
     1861 cttccctccc gtcctcggat gcggcgagtg cggcaaagat atctccgccg gcctgtatcg
     1921 cgcgctcgag agcaagcccc tccatgcgag cgaatcctac ctttcccgca cggagcgaaa
     1981 gcgcgctctt cgacgcaagt ttttttgcca gttccagcgt cttctcctca agcgtttctc
     2041 ttgccgtcat tgtatggacg aacccgagtt cgagcccctt cgatgcgggg atgagttcgc
     2101 cgaagaggac gtactgtagc gcgcgtttct ctcctatcca acgggcaagc tgatacccgg
     2161 gctcgaggca gatgagccct acgttgaccg ctgttgttcc gaagaccgcg tcctcggatg
     2221 cgacaacaaa atcgcaggca agtgcgagac ccgttccgtt ggcgagcgca tatccatgaa
     2281 cggaagctat aaccggcttg gtcaacgatg cgagcctgtg gttgtgcaga tccatgagct
     2341 tcaagaagcc ggggatttcg tgccgctctt tcgagagcag ctcccggagg tcgattcccg
     2401 tcgaaaagtg cttacctgct ccggaaacga tgatgacgcg aacctcgtca ttcgcgtcgg
     2461 ccgcacggaa cgcctcgtca agaccgatcg caagatcttc gttgaatgtg ttcatcgctt
     2521 ccggcctgtt cagttcgata cggcaaacgc cttcttttat gctgctgagc actgttgtca
     2581 tttttttctc ccttcagctt atttttttgt cgagccgtgg aaggcgctgc gccgacggac
     2641 agagagcgag accgcctctt gatgtgcaac gcaactcttc ggggagcatc cttcctgtgc
     2701 tgtcgacggc gtcgatcgtt tcgtccagag gcaccgggct tcggtacccg ccgagcacaa
     2761 gatccgcgca gagaaacgcc tgcgatgcca gcgcggcgtt tctgctgtgg cagggtattt
     2821 caaccactcc ctgcaccagg tcgcagacgg atcccatcat gttttgaaat actgtcgcgg
     2881 ctgcgtcgca cccctggcgc gcgcttcctc cggcggcctc aacaactgcg gctgcggcca
     2941 tcgctcccgc ggcgcctatc tctacctggc atcccgcaac ttctgcggcg aaagtacttc
     3001 tgatatcgtg aaagagtcct atgcctccgg cagcccaaag cgccatgagt acagcttttc
     3061 tgctgatttt caggtcctct gtgagcgttg tcagaacgcc gggaagcacg ccggccgcgc
     3121 cgcctgtggg agctgcgcag accactcctc cagaagcgtt agtatgca
//
LOCUS       DAOPKU010000346         3155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_34560_length_3155_cov_5.330000, whole genome shotgun sequence.
ACCESSION   DAOPKU010000346 DAOPKU010000000
VERSION     DAOPKU010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34560_length_3155_cov_5.330000"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..772)
                     /locus_tag="PK849_10475"
     CDS             complement(134..772)
                     /locus_tag="PK849_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPE66591.1"
                     /translation="MPIQRRLLFFLLLLLAGTASYWFFGPSRVNSWRMRNSPPAAFGP
                     VVAFGDSLTAGRGAGGAENSYPSALSSLLGRPVENMGVDGDTVARASERLEKVLELSP
                     SIVVVLLGGNDMLRRMNLDESFALLETMIRRIQQEGSLVFVAGLEGLPLLAPVGKRYF
                     EAAKNTGSVLIPDVLKGILGKPELMADGIHPNAAGYRVMAERIAKAMAPYLR"
     gene            complement(881..1078)
                     /locus_tag="PK849_10480"
     CDS             complement(881..1078)
                     /locus_tag="PK849_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66592.1"
                     /translation="MKKAGSDTIAEALRQRCIDVEYLVKENEGDGFLNEENSFDLYGV
                     MGRFSRASGESRRGATGCGKK"
     gene            complement(1265..2809)
                     /locus_tag="PK849_10485"
     CDS             complement(1265..2809)
                     /locus_tag="PK849_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE66593.1"
                     /translation="MRKVCTFTALLLLLALFVSPSFGADIGAMTPAKGTAVQWLDAHS
                     EVGAEVAKYIWHNPELGLGEHKSSTVLQEMLKAAGFKVESGVAGMETAFVASWGEGKP
                     VIGIHAEFDALPGLSQVPGLAEEKMVVEGCPGHGCGHNLFGSYSSMAAIAVKKAMEAN
                     GVKGTIKLYGTPSEETLVGKVFFVKEGVYKDADAVISWHPGTSNAVSYASSLAMDNFK
                     VRFYGKASHAASAPWAGRSALDAVELMNIGMNYMREHVRPESRIMYSIPDGGKAPNVV
                     PPFAEVWYFVRAPQYKTVKEIMDWTKKVAEGAALMTQTRMEFIPITAVWQYLPNQVLA
                     KVGFANAQLIGIPPFTDEDEKIAEPFSKSVKVEKGPFLSREFTQYDYDKPFSWATGGG
                     SIDEANTSWVVPMVRFSGSTLAQGTPGHSWQATAQNALPPAFKAALTVAKYMAATALD
                     LYANPSLLEEALAELKASNEKHGPFVDPAKDVPLPTFQLMHNVDESQVPVQTKDMPMP
                     DFSTLK"
     gene            complement(2884..>3155)
                     /locus_tag="PK849_10490"
     CDS             complement(2884..>3155)
                     /locus_tag="PK849_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66594.1"
                     /translation="GENAMNRRGSNQLREEAGERAARQICVFQQIIATRQKWTTRRLF
                     TLDCPIRVLTKATIMGNLNQRLTFVEFLLSCLYLKEVVGAAELLA"
BASE COUNT          613 a          915 c          900 g          727 t
ORIGIN      
        1 ctttaccggt tccgggatgt acttggcacg acacccctta ttctattttc gcctgcattc
       61 gcttacgatt cggtccagta tctcctgcat gacagcctcc tgtcgtcgac aatgagtatg
      121 ttctccggcg cgtttaccgg agatacggag ccatcgcttt cgcgatgcgc tcggccatga
      181 cgcggtaccc cgcggcgttg ggatgaatgc cgtccgccat taactccggt ttcccgagaa
      241 tacccttcag gacgtcgggg atgagcacgg aacccgtatt cttcgccgcc tcgaagtagc
      301 gcttgccgac cggcgcgagt agcggcagtc cctccagacc ggcgacgaag acaagcgatc
      361 cttcctgctg gatcctccgg atcatcgtct caaggagggc gaagctctcg tcgaggttca
      421 tccggcgcag catgtcgttt ccgccgagca gcacgacgac tatcgacggt gaaagttcca
      481 gcactttttc caggcgttca gaagctcttg cgacggtgtc cccgtcgacg cccatgtttt
      541 ccaccgggcg tcccagcagc gaggagagcg cggaggggta ggagttttcc gctcctcccg
      601 cccctctgcc cgccgtcagg ctgtcgccga aggcgaccac ggggccgaat gccgccggag
      661 gggagttcct catcctccac gagttgaccc gcgagggacc gaagaaccag taagacgcag
      721 ttccggcaag caggagcaga agaaaaaaca gaagacgtct ctgaatcggc attgtgctct
      781 ctcacctccg aattggatgc gaaaaattgt tttcctaatg atccggtgcg tttcgggaaa
      841 gtcgctgcgt ctcaaaagca caccgtgcgc cctcgaatat tcacttttta ccgcagccgg
      901 ttgctccgcg gcgagactcc ccggaagccc gggaaaaacg ccccatgacg ccgtagaggt
      961 cgaagctgtt ctcctcgttg aggaatccgt cgccttcatt ctccttgacc aggtactcca
     1021 cgtcaataca tcgttgcctc aaggcttccg caatcgtatc cgatccggcc tttttcacgc
     1081 ggggggatgt tggctcccag gtaacgacga gcagcgccca agagtaacga ccggctccgc
     1141 aaggttcgcc agtttgctga acctttgcgt tttccggtcg aagaagcggc agtgcacacg
     1201 gtctgttatg cagcgaaatg cgtgccggag gaaaagctcc ctccggcaca tggtgtcaac
     1261 agacttattt caaagtggaa aaatcgggca tgggcatgtc ttttgtctga acgggtacct
     1321 gcgactcgtc aacattgtgc atgagctgga atgtgggaag gggtacgtct tttgccggat
     1381 caacaaaggg gccgtgtttc tcgttggagg ctttcagctc ggccagcgcc tcctcaagaa
     1441 gcgaggggtt tgcataaagg tcgagggcgg tcgcggccat gtatttcgcc acggtaagag
     1501 ctgccttgaa cgctggggga agcgcgttct gcgctgtggc ctgccaggag tgccccggag
     1561 ttccctgggc gagagtcgag ccgctgaagc gaaccatcgg caccacccag cttgtattgg
     1621 cctcatcgat ggagccacct ccggttgccc aggagaatgg cttgtcgtag tcgtattggg
     1681 tgaactctct ggagaggaag gggcctttct ccaccttgac gctctttgag aagggttcgg
     1741 ctatcttctc atcttcgtcg gtgaacgggg ggatgccgat gagctgcgcg ttggcgaatc
     1801 cgaccttcgc cagcacctgg ttgggcagat actgccagac ggcggtgatg ggaatgaact
     1861 ccatcctggt ttgcgtcatg agggcggcgc cttcagctac tttcttcgtc cagtccatga
     1921 tctccttgac ggtcttatac tggggggcgc ggacgaagta ccagacttca gcgaacggag
     1981 gtacgacgtt aggagctttt ccgccgtcgg gaatggagta catgattctc gactcgggac
     2041 ggacgtgctc ccgcatgtag ttcatgccga tgttcatcag ttcgaccgcg tcgagcgcgc
     2101 ttctgcccgc ccatggggcg gaggctgcgt gcgaagcttt tccgtagaag cggaccttga
     2161 agttgtccat tgcgagggag gatgcgtacg aaacggcgtt cgacgtcccg gggtgccagg
     2221 agatgacggc gtctgcgtct ttatatacac cttccttcac aaagaatacc ttgccgacga
     2281 gcgtttcttc ggatggagtc ccgtagagtt tgatcgttcc ttttactccg ttcgcctcca
     2341 tggctttttt cacggcgatg gccgccatgg agctgtacga gccgaaaaga ttgtgtccgc
     2401 acccgtgtcc cgggcaccct tcgaccacca ttttctcttc ggccagcccg gggacctgag
     2461 agagaccggg gagcgcgtcg aattccgcat gaatgccgat gaccggtttc ccctcgcccc
     2521 agctcgcgac gaacgctgtc tccatgccgg cgacgccgga ttcgaccttg aacccggccg
     2581 ctttgagcat ctcctgaagg acggtggagc tcttgtgttc gccgagtccg agttcggggt
     2641 tatgccaaat gtattttgcg acttcggctc cgacttcgga atgcgcgtca agccattgga
     2701 cggcggttcc cttcgccggc gtcatcgcgc cgatatccgc gccaaacgac ggggatacga
     2761 aaagagcaag aagcagcagc agcgctgtga atgtacagac ctttcgcata gcgttacctc
     2821 cttttgatcc aatactaccc gccggagaag gatcttccgg caaacgtctg gaatgcgcat
     2881 tcttcacgca aggagctctg ccgcgccaac cacctccttt aaataaagac aggaaagcaa
     2941 aaactccacg aaggtcagcc gttggttcag attacccata atagtagctt ttgtcaatac
     3001 tcttataggg caatctagtg tgaacagcct tctggttgtc catttttgcc gtgttgctat
     3061 aatttgctga aagacacata tttgtcgtgc agcgcgctct cctgcctctt cccttagctg
     3121 gttgcttcct cgtctgttca ttgcgttttc ccctc
//
LOCUS       DAOPKU010000347         3155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_34566_length_3155_cov_4.479355, whole genome shotgun sequence.
ACCESSION   DAOPKU010000347 DAOPKU010000000
VERSION     DAOPKU010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34566_length_3155_cov_4.479355"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PK849_10495"
     CDS             complement(<1..362)
                     /locus_tag="PK849_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66595.1"
                     /translation="MLETLRTAIRSLLSNKIRSALTMLGIIIGVAAVITMVAIGAGAS
                     RNIQQFISGVGSNILIIMPGAASVGGVRQQAGSGATLTTEDASALEREAYALKGVAPE
                     IYSRTQLVYGNMNWSTLVT"
     gene            complement(355..1047)
                     /locus_tag="PK849_10500"
     CDS             complement(355..1047)
                     /locus_tag="PK849_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66596.1"
                     /translation="MSLIDVRDIVKIYHTGDVELRALDGVSFSVGQGEFVSIMGHSGS
                     GKSTMMNILGCLDVPDEGEYELDGERTGRLSKDALAKIRNAKIGFVFQGFNLLPRATA
                     LENVELPLVYAGVPGKKRKERGKTALERVGLADRMGHRPSQMSGGQQQRVAIARALVG
                     ETPLILADEPTGNLDTKTSAEIMDLFVALNREGKTIILVTHEPDIAEYSARTIRFQDG
                     RILADERRENHA"
     gene            complement(1044..2294)
                     /locus_tag="PK849_10505"
     CDS             complement(1044..2294)
                     /locus_tag="PK849_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPE66597.1"
                     /translation="MKKIIVLLLLLAGGAGYLFLSDGGATEYTFRTAALESGNIVAKV
                     TATGKLSAVTVVEVGTQVSGTVKEIYADFNDHVKKGQLIALIDPDVLSAKLEESKANL
                     TVARASVARAKANLADSQRNLKRNKELWSRNLIAKSELEAAETTHALNRASLQEAEAR
                     VLQVQAALRQSESNLGYTRILSPEDGVIISRLVNVGQTVAASLSTPTLFSIAKDLTDM
                     QIETSVDEADIARVKEGQEVEFTVDAHRGTVFPGKVKQVRIAPGTTDNVVTYPVIISV
                     ANPELKLKPGMTANVSIITERRTGVLRVPMAALRFTPPPTEAPVAVATATSSPFAPSM
                     PRRPRGGGAGNGQGGGAGTNGAFSSIWTVRDGKLAEQINFRAGIGDGSFIEVAGSKEL
                     KEGDLLAIAYSEKPKVSFWKRIFQ"
     gene            complement(2291..>3155)
                     /locus_tag="PK849_10510"
     CDS             complement(2291..>3155)
                     /locus_tag="PK849_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE66598.1"
                     /translation="KGVKDAYYTLLLNGLKREQAESAVRTYEKHLEKAKGFYEAGIKA
                     RFDVTKAEVDLSNARITLVSAEASLATARAALSNAVGIQLGDVEAVSGFLAPRSLPDE
                     PFAVEQAMANRPDIRSALLKSESGKLSVAIAAKGNAATVSLSGSANLSGSEWPLNDTF
                     RATVTLSVPVFDGGLTDAKVAEARYGAQGADAALRRVEQTVLYDVRSTLLSVKEAEAR
                     IPVAELLVRQAEENLTLAEGRYEMGVGNVLEVADAVLAFTSAKVSLYQAQHDYSASLA
                     ALEKTLGGEFE"
BASE COUNT          449 a         1064 c         1048 g          594 t
ORIGIN      
        1 gtcaccaggg tggaccagtt catgttgccg tagacgagct gcgtccggct gtatatctcg
       61 ggagctacgc ccttcagcgc gtacgcctcg cgctcgagcg ccgaggcgtc ctccgtggtc
      121 agcgtcgcgc cggagcccgc ctgctgccgg acgcctccga ccgaggccgc tcccggcatg
      181 atgatcagaa tgttgctccc cacgccggag atgaactgct ggatgttgcg gcttgccccc
      241 gcgccgatgg cgaccatcgt gatcacggcg gcgacgccga tgatgatgcc gagcatggtg
      301 agcgcggagc gtatcttgtt ggagagcagc gagcggatcg cggtgcggag cgtctcaagc
      361 atggttttct ctcctctcgt cggcgaggat gcgcccgtcc tggaagcgga tggtgcgcgc
      421 gctgtactcg gcgatgtccg gctcgtgggt gacgaggatg atggtcttcc cctcgcggtt
      481 cagggcgacg aagaggtcca tgatttcggc gctggtcttc gtgtccagat tgcccgtggg
      541 ctcgtcggcg aggatcagcg gcgtctcgcc gacgagggcg cgggcgatgg ccacgcgctg
      601 ctgctgcccc ccggacatct ggctcgggcg gtgccccatc ctgtcggcga gccccacccg
      661 ttcgagggcg gtcttccccc gctccttgcg cttcttcccc ggcacgcccg cgtagaccag
      721 cggcagctcg acgttttcga gcgccgtggc gcgggggagg aggttgaacc cctgaaagac
      781 gaagccgatc ttcgcgttgc ggatcttcgc cagcgcgtcc ttcgagaggc ggcccgtccg
      841 ttctccgtcc agctcgtact ccccctcgtc cggcacgtcg aggcagccga ggatgttcat
      901 catggtggac ttccccgagc cggagtgccc catgatggag acgaactccc cctgcccgac
      961 ggagaaggag acgccgtcga gcgcccgcag ctccacgtcg ccggtatggt agatcttgac
     1021 gatgtcgcgg acgtcgatca ggctcattgg aagatcctct tccagaacga gaccttcggc
     1081 ttctcggagt aggcgatggc gaggagatcc ccctccttca gctctttcga cccggcgacc
     1141 tcgatgaacg agccgtcgcc gatccccgcg cggaagttga tctgctctgc gagcttcccg
     1201 tcgcgcacgg tccatatgga ggagaacgcc ccgtttgttc ccgcgccgcc tccctgcccg
     1261 ttgccggctc cgccgcccct ggggcgccgc ggcatggagg gggcgaaggg cgagcttgtg
     1321 gctgttgcaa ctgctacggg agcctccgtc ggcggagggg tgaagcgcag cgccgccatc
     1381 ggcacgcgga gcacgccggt tctgcgttcg gtgatgatgg agacgttggc cgtcatcccc
     1441 ggcttcagct tcagctcggg attggccacc gagatgatga ccgggtaggt gaccacgttg
     1501 tccgtcgtcc ccggcgcgat gcgcacctgc ttcaccttgc cggggaagac ggtccccctg
     1561 tgcgcgtcca cggtgaactc cacctcctgc ccctccttca cgcgggcgat gtccgcctcg
     1621 tcgacggagg tctctatctg catgtccgtc aggtccttgg cgatggagaa gagcgtcggc
     1681 gtggagaggc tcgccgcgac ggtctggccg acgttgacca ggcgcgagat gatcacgccg
     1741 tcctccggcg agaggatgcg cgtgtacccc aggttggact ccgactggcg cagggccgcc
     1801 tgtacctgga gcacgcgcgc ctccgcctcc tgaaggctcg ccctgttgag ggcgtgcgtg
     1861 gtctccgccg cctccagttc gcttttcgcg atcaggttgc gcgaccacag ctccttgttc
     1921 cgcttcagat tcctctggct gtcggcgagg ttggccttcg cccgagcgac cgacgccctg
     1981 gcgaccgtga ggttcgcctt cgactcctcc agcttcgcgc tgaggacgtc cgggtcgatg
     2041 agcgcgatga gctgcccctt cttcacgtgg tcgttgaagt cggcgtaaat ctccttgacg
     2101 gtcccggaga cctgcgtgcc gacctcgacc acggtgaccg cgctcaactt tccggtggcc
     2161 gttacctttg cgacgatgtt ccccgactcc agcgcggcgg tgcggaacgt gtactccgta
     2221 gcgcccccgt ccgagaggaa gaggtagccg gcgcccccgg ccagcagaag cagcagtacg
     2281 ataatttttt tcattcgaac tcacccccga gggtcttctc cagcgcggcc agcgaggcgc
     2341 tgtagtcgtg ctgtgcctga tagaggctta ccttcgcgct ggtgaaggcg agcacggcgt
     2401 ccgccacttc gaggacgttc cccacgccca tctcgtagcg tccctccgcg agcgtcaggt
     2461 tttcctccgc ctggcgcacc agcagttccg ccaccgggat gcgcgcttcg gcctctttca
     2521 cggagaggag cgtcgagcgg acgtcgtaga ggacggtctg ttcgacgcgg cgcagcgcag
     2581 cgtccgcccc ctgcgcgccg tagcgggcct ccgccacctt ggcgtccgtc agcccgccgt
     2641 cgaagacggg gacggagagc gtgaccgtgg cgcggaaggt gtcgttgagc ggccactccg
     2701 aaccggagag gttcgcggag cccgaaaggg agaccgtcgc ggcgttcccc ttcgcggcga
     2761 tggcgaccga gagcttgccc gactcgctct tcaaaagagc ggagcggatg tccggcctgt
     2821 ttgccatggc ctgctcgacc gcaaagggct cgtcggggag cgaacggggc gcgagaaagc
     2881 cggagacggc ttcgacgtcg ccgagctgga tgccgaccgc gttcgacaga gctgcacgcg
     2941 ccgtggcgag cgacgcctcc gcggagacga gggttatccg ggcgttgctc aggtccacct
     3001 cggccttggt gacgtcgaac cgcgccttta tcccggcctc gtagaagccc ttcgccttct
     3061 ccagatgctt ttcgtaggtg cgcacggcgc tctccgcctg ctcgcgcttc agtccgttca
     3121 gcaagagcgt gtagtaagcg tccttcacgc ccttc
//
LOCUS       DAOPKU010000348         3153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_34597_length_3153_cov_5.230471, whole genome shotgun sequence.
ACCESSION   DAOPKU010000348 DAOPKU010000000
VERSION     DAOPKU010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34597_length_3153_cov_5.230471"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PK849_10515"
     CDS             complement(<1..300)
                     /locus_tag="PK849_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66599.1"
                     /translation="MNRFRKVCVSSLLLLVLLASVAVATETKPEPAKAEPSPAAVEEK
                     APVADPETVLATVGSAEIKQKDVTAIIGRLDPQRAAMYDNEMGRRAILEELVNMEL"
     gene            complement(420..590)
                     /locus_tag="PK849_10520"
     CDS             complement(420..590)
                     /locus_tag="PK849_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66600.1"
                     /translation="MTNFAQSVAPYILYTCSLVLLPLGVNRFRAGSPVKGFLDFVGAG
                     VIAVMAWSLANP"
     gene            900..1625
                     /locus_tag="PK849_10525"
     CDS             900..1625
                     /locus_tag="PK849_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE66601.1"
                     /translation="MTKRRLPVRVLVAEDDPMVCSLHVQTIQQVHGFTVAGTVGDGIE
                     MVRFFESSSADLIVMDIFMPRLSGLDALKKLRAEGNVADVILVSAGKHPQLVSQAKLL
                     GAFDYLIKPYSLKRFRTSLEAYLENRTKMPSETEEATQEALDSLFEIASANRSPGGSE
                     KLPKGLQPGTLSLVMKELTDDKGLSAEEICTRLSISRSTAWRYLDHLSKTGAVRIQFE
                     YNGPGRPLKRYLLRESIRSELLV"
     gene            1622..2932
                     /locus_tag="PK849_10530"
     CDS             1622..2932
                     /locus_tag="PK849_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE66602.1"
                     /translation="MKQTERPSFMPWKTQYSRCATETPRPSPTQKNTAAEESVFSVAA
                     LCAETLKALHQRIRKRKPRIELDIDSSIPRLARGNGAQIENILHLLLSNVIALSLDAA
                     VKLSVVTRRFTAEGLEVLFSIEWAHSGVALSADPVIEGNAPPGLSYSICSSVIAAMGG
                     RLHVKKQEENMVISFFIPLSTAGISEDAENGGAPVLLVEDNAMNRKIIASSLEHGGYS
                     VFTASSGQEALNALFDKEWSIILMDIQMPGMDGIQTTHAIRAEEKKKGRRTPIVAVTA
                     YARPEDRERCLAAGMDDFLAKPVKLETLLSTVERVTGIPSARKSAVRGPDAASAILSF
                     TEGDAELARKIIGEFLALAPPLLLSMRDAFDRKDRETLRVLSHRLKGNLAYFGEGEAF
                     LLAEALHRELDNLSDEAVAATLLRLLSESEYLIEALENKEEAAR"
BASE COUNT          762 a          905 c          835 g          651 t
ORIGIN      
        1 gagctccatg ttgaccagtt cctcgagaat cgctctgcgc cccatctcgt tgtcgtacat
       61 ggccgccctc tggggatcga gacggccgat aatcgcggta acatccttct gcttgatctc
      121 ggccgatccg acagtagcga gcacggtctc gggatcagca accggggcct tctcttcgac
      181 tgccgcagga gacggctccg cctttgccgg ttccggcttc gtctcggtcg caactgcgac
      241 ggacgccaga agaacaagga gcaacagcga tgacacgcat acttttctga aacgattcat
      301 tcaaaaacct ccctaaaata atccttcacg gaatttggaa caaaacggac agagtgtacc
      361 atagaaaaga aaaaaagaaa cgtacaaaat gctgaaaccc cgtatcatgc catcagcgcc
      421 taggggttcg caagactcca ggccatgacg gcaatgaccc ccgcgccgac gaaatcgaga
      481 aaccccttca caggactgcc cgccctgaag cgattgaccc cgagcggaag aagaacgagc
      541 gaacacgtat acaggatgta cggcgcgact gactgcgcaa aattcgtcat cgagacccct
      601 cctttggacg tgttaataat gcagtgcatg gagaagaaag tcaagcggga tgagcgtttc
      661 cgaagccaga gagaaaaaag ggggttccgc tcctcgtcga aaacccgggg caggttttcg
      721 ggaagcggaa ccagtgcgtg gggaagttca tcaaccgaac cgggaatgca atttatgtac
      781 cattcattct gaatcacaaa cctaaaaaaa gcaagagttt tgttcattga ttttcttttt
      841 gttcattgtg tttacgaacg cggagcgaga gcatataatt caatcaaggg gtgaggaaaa
      901 tgacgaaacg aagactaccc gtcagagttc tcgtagccga ggacgatccg atggtatgca
      961 gccttcatgt acagacgatt cagcaagtgc acggctttac cgtagctgga accgtcggcg
     1021 acggcattga gatggtgcgt tttttcgagt cgtcttcagc cgatcttatc gtcatggata
     1081 ttttcatgcc ccgtctttcc ggtctcgacg cgctgaaaaa actccgcgcc gaggggaacg
     1141 tcgccgacgt cattcttgta tcggccggca agcatccgca actggtatcc caggccaagc
     1201 tgcttggagc gttcgactac ctgatcaagc cctattcgct gaaacgcttc cggacatctc
     1261 ttgaagccta ccttgaaaac aggacaaaga tgccgtcgga gactgaggaa gcgacccagg
     1321 aggcgctgga ctctctcttc gaaattgcct ccgcaaacag atctcccggg gggagcgaga
     1381 agctgcccaa ggggctgcag ccgggaacgc tctcccttgt aatgaaagag ctcaccgacg
     1441 acaaaggcct ttctgctgaa gagatatgca ctcgtctttc tatctcacgt tccaccgcat
     1501 ggaggtacct cgaccacctc tccaaaacag gcgccgtcag aatccagttc gaatacaacg
     1561 gccccggaag gccgctgaaa cgctatctcc tgcgcgagag catcagatcg gagttacttg
     1621 tatgaaacag accgaacgtc catcctttat gccgtggaag acccagtaca gccggtgtgc
     1681 aaccgagacg ccgcgcccct cgcccacaca aaaaaatacc gcggcggaag agagcgtttt
     1741 ttctgtcgcc gctctctgtg ccgaaaccct gaaagcgctt catcaacgta ttcgaaaaag
     1801 gaagccgcgc atcgagctcg acatcgactc ctccatcccc cgcttggcga ggggaaatgg
     1861 agcgcagata gagaacatac ttcaccttct tctctcgaac gtcatcgcgt tgagtttgga
     1921 cgccgccgta aaactctccg ttgtcacacg aaggtttaca gccgaggggc tggaagttct
     1981 tttttccatc gaatgggcac acagcggcgt tgctttgtcg gccgatcccg ttattgaagg
     2041 aaacgcgccg cccggcctga gttattcaat ttgctcgtcg gtcatcgctg caatgggcgg
     2101 ccgtttgcac gtgaaaaaac aggaagagaa catggtcatc tcgtttttta ttccgttatc
     2161 aaccgccggt atctccgagg atgcggagaa cggcggcgcg ccggttctgc ttgtggagga
     2221 caacgcgatg aacaggaaga tcatcgcgtc cagcctcgaa cacggagggt actcggtttt
     2281 caccgcttct tccggtcagg aagcgcttaa cgccctcttc gacaaagagt ggagcatcat
     2341 tctgatggat atccagatgc caggaatgga cggaatacag acgactcacg ccatccgcgc
     2401 cgaggagaag aaaaaggggc ggcgcactcc gatagtcgcc gtcaccgcgt acgccagacc
     2461 cgaggataga gagcgctgcc ttgccgcggg gatggacgac ttcctcgcaa aaccggtcaa
     2521 actggagacg cttctctcca ccgtcgagag agtcaccggc atcccctccg ccagaaagag
     2581 cgcggtaagg ggaccggacg ccgcctcggc cattctctcc tttacggaag gggatgccga
     2641 gctggcgcgg aaaatcatcg gcgagttcct cgcgctcgcc ccgccgctgc tgctctcgat
     2701 gcgcgatgcc ttcgaccgga aggataggga gacgctccgt gtgctctctc acagactcaa
     2761 ggggaatctg gcctatttcg gcgaaggaga agcctttctc cttgcggaag cgcttcaccg
     2821 ggagctcgac aacctctccg acgaagccgt cgcggcaacc ttgctccgcc ttctttctga
     2881 gagcgaatac cttattgaag cactggaaaa caaagaggaa gcagcccgat aacttcggac
     2941 gcttcccctt gcttttcttt attacctgcc ggctggtatc atctacaacc ggctttttgg
     3001 tctagccctt cctcataccg cccgcaagct tccctgcgat cagtcccgcg acagtcggaa
     3061 gcagcacggt acattttttc caatggaaca ggccattacg ccttgaaggc gaagacggtg
     3121 ccggctccca tagttgcgaa tttttcatcg aac
//
LOCUS       DAOPKU010000349         3149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_34668_length_3149_cov_4.721073, whole genome shotgun sequence.
ACCESSION   DAOPKU010000349 DAOPKU010000000
VERSION     DAOPKU010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34668_length_3149_cov_4.721073"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PK849_10535"
     CDS             <1..1482
                     /locus_tag="PK849_10535"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPE66603.1"
                     /translation="RVYASMAPTWACEFPGLDSGSLVAALKKLGFTGVGETALGAHEV
                     CESLAKTLSEAEPGLFLSTTCPSVVSYVERYLPELLGSLSTVPSPVEAHCRIFKDRVD
                     PEGAVVFIGPCIARKDESDRSVKTIDAALTFRELRRWLHDESVAPEKCVPGPEDHFFP
                     EPTGDGFLYPVEGGMCDMIRANGVDKKVRFVTVSGLQNLRRYLRGLSPEGLGAPVFLE
                     CFACLGGCINGPGGTWDEPGLLRRNRVEAYANLSNAPRHTIFLEDEGSAMREEEMKEA
                     LRRIGKTRPEDEVNCGGCGYASCRELAAALALGRAEPEMCVIHMRNQAQKKANALLRT
                     MPSGVVIVDKSLHIVECNERFARLFGESAMLVYRAEPGLKGCILGRILPFTHLFELVL
                     EKGQDIHLDHYRTGDTLLDITLFPIEPGETVGAVMLDVTHKELKRDKIAQRAAEVIER
                     NILTVQEIACRLGEHMADTEMLLRSIAEGYSESDEDVSQEDAR"
     gene            1479..2660
                     /locus_tag="PK849_10540"
     CDS             1479..2660
                     /locus_tag="PK849_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPE66604.1"
                     /translation="MKQEEPLFLDVDFDQRKKAGQYVCGDSFFSRKLPGRNRVVSVLS
                     DGLGSGVKASILSSMTGTMALRFMEGDMEILRSAEVMMDALPVCRKRGISYATFTIVD
                     AVLHGWTRVIEMDNPSFMLVRNGRLLAPGKREMASSRWKDRKITVNEFFARPEDRIIL
                     FSDGVTQAGMGSWANPTGWGQEKCAAFILDTLRERPGISSRELSRRVVSEALVREPHE
                     RAGDDVTCAVMYFRSPRKLLVLSGPPFEKKRDAEFARMLDTFDGRRAVCGGTTAEIVA
                     RELGRPLVTDLATARGGLPPISTMEGADLITEGILTLTEVSRILEGKKITREKTSASR
                     LAALLEESDRIRFVVGTRVNEAHQDPTLPVELEMRRNIVRTIARTLEERYLKEVELTF
                     V"
     gene            2694..>3149
                     /locus_tag="PK849_10545"
     CDS             2694..>3149
                     /locus_tag="PK849_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66605.1"
                     /translation="MHVRNAKNGTITTLYERSLKRFEYFTRRLVNEMHYRRTPVWILR
                     PACVLFAAFILLASSSFAAAGAEKRILLLHSFHQGYEWTDSLHQGVVEELQNSGDKDI
                     ELFVEYLDCIRNPGPAHEEMMAELFRRRYAERGVLFDAIICTDDDALVFL"
BASE COUNT          567 a         1005 c         1034 g          543 t
ORIGIN      
        1 agggtgtacg cctccatggc gccgacgtgg gcctgcgagt tccccggtct cgactccggc
       61 tcgctcgtgg cggcgctgaa gaaactcggt ttcaccggag tgggggagac cgcgctcggc
      121 gcgcacgagg tctgcgaatc ccttgcgaag acgctctcgg aggcggagcc ggggctcttt
      181 ctctccacca cctgtcccag cgtggtgtcg tacgtggagc gctacctccc cgagctgctg
      241 ggaagcctct ccacggttcc ctccccggtc gaggcccact gccgcatctt caaggatcgc
      301 gtggatcccg aaggcgccgt cgtcttcatc ggcccctgca tcgcccgcaa ggacgagtcc
      361 gacaggtcgg tgaagaccat cgacgccgcg ctcaccttcc gggaactgcg ccgctggctg
      421 cacgacgaat cggtcgcgcc ggagaagtgc gttccggggc cggaggatca cttcttcccc
      481 gagcccaccg gcgacggatt cctctaccct gtcgaggggg gcatgtgcga catgatccgt
      541 gcgaacggag tagacaagaa ggtgcgcttc gtcacggtct cggggctgca gaacctgcgc
      601 cgctatcttc gggggctctc tcccgaaggg ctgggcgcgc ccgtcttcct ggagtgcttc
      661 gcctgtcttg gagggtgcat caacggacct ggcggcacgt gggacgagcc ggggcttctc
      721 cggcgcaacc gcgtcgaagc ctacgcaaac ctctcgaacg ctcccagaca cactatcttt
      781 cttgaggatg aggggtccgc gatgcgggag gaagagatga aagaagccct tcgccgcatc
      841 gggaaaacgc gcccggagga cgaggtgaac tgcggggggt gcggctacgc ctcctgccgg
      901 gagctcgcgg ccgcgctggc gcttggtcgg gcggagcctg agatgtgcgt catccacatg
      961 cggaaccagg cgcagaagaa ggcgaacgcg cttctccgga ccatgccctc cggcgtcgtg
     1021 atcgtggaca aaagcctgca catcgtcgag tgcaacgagc gcttcgcccg tctcttcggc
     1081 gagagcgcga tgctcgtcta ccgcgccgag ccggggctga aagggtgcat cctcggcaga
     1141 atcctgccct tcacccacct cttcgaactc gtgctcgaaa aggggcagga tatccacctc
     1201 gaccactacc gcaccggcga cacgctgctg gacatcacgc tcttccccat agaaccgggg
     1261 gaaacagtcg gcgccgtgat gctcgacgtc acccacaaag agctgaagcg cgacaagatc
     1321 gcccagcgcg ccgcggaggt catcgaacgg aacattctca cggttcagga gatcgcgtgc
     1381 aggctggggg agcacatggc ggacacggag atgctgcttc gttcaatcgc cgaggggtat
     1441 tccgaatcgg acgaggacgt ttcgcaggag gacgcgcgat gaaacaggaa gagcctcttt
     1501 ttctcgacgt cgatttcgac cagcgaaaga aggccgggca gtacgtctgc ggcgacagct
     1561 tcttctcccg caagctgccg gggcggaaca gggtggtttc ggtgctctcc gacggtctcg
     1621 gcagcggagt gaaggccagc attctctcgt cgatgacggg gacgatggcg ctccgtttca
     1681 tggaggggga catggagatc ctccgctcgg cggaggtgat gatggacgcg ctccccgtct
     1741 gccggaagcg cggcatcagc tacgccacct tcacgatcgt cgacgcggtg ctccacggct
     1801 ggacgagggt gatcgagatg gacaacccct ccttcatgct ggtgcggaac ggacggctcc
     1861 tcgcccccgg aaagcgggag atggcctcct cccgctggaa ggaccggaag atcacggtga
     1921 acgagttctt cgcccgcccg gaagaccgga tcattctctt ctccgacggg gtgacgcagg
     1981 ccggaatggg atcgtgggcc aacccgacgg ggtgggggca ggagaagtgc gccgccttca
     2041 tcctcgacac gctccgggag cgcccgggca tctcctcgcg ggagctgtcg cgccgggtcg
     2101 tctccgaagc gctcgtcagg gagccgcacg agcgggccgg ggacgacgtc acctgcgccg
     2161 tgatgtactt ccgctcgccg aggaagctgc tcgtgctctc agggccgccg ttcgagaaga
     2221 agcgggacgc cgagttcgcc cggatgctgg atactttcga cggacgtcga gccgtctgcg
     2281 gcggtacgac cgcggagatc gtcgcccgcg aactgggccg tccgctcgtc accgaccttg
     2341 caaccgcgcg cggcggcctg cccccgatct cgaccatgga gggggccgac ctgatcaccg
     2401 agggcattct cacgctgacg gaggtctcca ggattctcga aggaaagaag attacccgcg
     2461 agaagacctc ggcatcgcgc ctcgccgcgc ttctggagga gagcgaccgt atccgcttcg
     2521 tcgtggggac gagagtgaac gaggcgcacc aggaccccac gcttccggtg gagctggaga
     2581 tgcgccggaa catcgtccgc accatcgccc gcacgctgga ggagcgctac ctgaaggagg
     2641 tcgagctgac gttcgtgtag aaaacggtgt ttcaccggcg gagggccccg gaagtgcatg
     2701 tgcggaacgc gaaaaatggt acaataacaa cactttacga gcggtccctg aagcgtttcg
     2761 aatacttcac ccgaaggctg gtgaacgaga tgcactacag acgtactcct gtttggatcc
     2821 ttcgccccgc gtgcgtgctc tttgccgcgt tcatccttct tgcatcgtcc tccttcgcgg
     2881 ccgccggggc cgagaagcgc attctcctcc tgcactcatt ccaccagggg tacgaatgga
     2941 ccgactcgct ccaccagggg gtcgtcgagg agcttcaaaa ctccggcgac aaggatatcg
     3001 aacttttcgt ggagtacctc gactgcattc gcaaccccgg accggcccac gaggagatga
     3061 tggcggagct gttccggcgc aggtatgcgg aacgcggcgt cctcttcgac gcgatcatct
     3121 gtacggacga cgacgccctt gtctttctg
//
LOCUS       DAOPKU010000350         3144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_34745_length_3144_cov_4.418582, whole genome shotgun sequence.
ACCESSION   DAOPKU010000350 DAOPKU010000000
VERSION     DAOPKU010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34745_length_3144_cov_4.418582"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PK849_10550"
     CDS             complement(<1..389)
                     /locus_tag="PK849_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPE66606.1"
                     /translation="MAMIQVVRFDPELDPGRVLAWRYRNPEDPARGDELGTWAQLVVE
                     ESQEAILFRNGQALDLFGPGRHTLKTANMPLRSKAVNLPFGGESPFKAWVWFVNKVHS
                     LDVKWGTPSPVQLKDPKYGVLLPVRSYG"
     gene            545..1293
                     /gene="larB"
                     /locus_tag="PK849_10555"
                     /pseudo
     CDS             545..1293
                     /gene="larB"
                     /locus_tag="PK849_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661208.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
     gene            complement(1411..2643)
                     /gene="pepT"
                     /locus_tag="PK849_10560"
     CDS             complement(1411..2643)
                     /gene="pepT"
                     /locus_tag="PK849_10560"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPE66607.1"
                     /translation="MPSVLDRFLKYIRIPSQAAHDAGKVPSTPGQMTLARELGEELKS
                     LGLADVVVDEHAYVTATLPGNTKGAPVIAFMAHLDTALEVTDDTVRPRLVENYDGGEI
                     ILNEADGVTLSPSTFPEMLLYKGETLVVTDGTTLLGADDKAGIAEIMAALEIMVARPE
                     LPRGDVKVAFAPDEEIGHGASLLDLKKFGADFAYTVDGGAVGEISYETFNAAKAKIVI
                     HGRSVHPGKSKDKMVNSILLGMELAAMFPANETPAHTEEYEGFFHLLGFTGNVETTEM
                     NYIIRDHSAEKFAERKKAVEAAVTAINKKYGEGRAELTMTDQYYNMLDKIKPQMHIVE
                     TAMEAMRRCGIEPKIIPVRGGTDGSQLSYRGLLTPNIFTGGHNAHGKHEFISVASMEK
                     AVAVVLKIVELYAGKSVR"
     gene            complement(2648..>3144)
                     /locus_tag="PK849_10565"
     CDS             complement(2648..>3144)
                     /locus_tag="PK849_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE66608.1"
                     /translation="LDDKLCVACIFGALKALVESKRLPARTTVLYISQYEEVGHGISA
                     MPKDVEEHLAVDVGIVAPPANSREDAVTIVARDSRTPYGFEFRKLLADLAAERSIPFR
                     VETHFRYGSDASVAALAGFDADFACFGPGVDASHSYERTTIQAVKACCDLLTAYLVTD
                     RKGV"
BASE COUNT          599 a          982 c          938 g          625 t
ORIGIN      
        1 ccgtacgacc tgaccggtag taacacaccg tatttgggat ccttaagttg cacaggagag
       61 ggagtacccc acttcacgtc aagtgaatgc accttgttta cgaaccagac ccacgccttg
      121 aatggcgact ctccgccgaa gggcagattg accgccttcg aacgcaacgg catgttcgcc
      181 gtcttcaacg tatgccttcc gggaccgaaa aggtcgagcg cctgcccgtt ccggaagagg
      241 atcgcctcct gcgactcctc cactacaagc tgcgcccacg tccccagttc atcgcccctc
      301 gccggatcct ccggattccg gtagcgccac gccaaaaccc tccccgggtc gagttccggg
      361 tcgaaccgga ccacttgaat catcgccatc gcacccaccg ccttcgttcg gagttatccc
      421 tcgggagcat atcgctgtat ctcttcgaat gatattatgc taccataccg ccagtcccgt
      481 aaaggagagc cggacgaaaa tccagcagag cgggacgttt tcttgaaggg aatgattata
      541 agctatggat gaacagcagc tgctctctct ccttcgcaaa gtcaaggaag gaacgctcga
      601 cgagtcggac tttgtccgcc agatgaaggc gctgccctac cgcgacctcg gcgaggtcaa
      661 gttcgatacg caccgccagc tccgcaaggg tttcgctgag atcgtctact gcccgggcaa
      721 gagcgacgag cagatcgaat cgatcgcgaa cgccttcgcc gatacgcaga ccaatattct
      781 tttctcccga gcgacagcac atcaattcga actggtacag gcggcgatac ctgacgcggc
      841 cttccatgaa aaggcgcgca tcatcgccgt ccggaaaaaa gattttcccc tgtgccgcgg
      901 cctcaccgtg atcaccgccg gaagcagcga tatccccgtg gccgaggagg ccgccgtgac
      961 ggcggagtat atggggtgcg ccgtagaaag gctctacgac gtcggcgtcg cgggactgca
     1021 ccggcttttg gcgcatgtgg acgtgctgca gaactccaag gcgatcgtcg cggtcgccgg
     1081 aatggaggag cgctcgccag cgtcgtggga gggctcgtcg cctgcccggt cgtcgccgtc
     1141 ccaacgagca ccggatacgg tgcgaatctc ggcggtatcg cgccgctgct caccatgctc
     1201 aattcgtgcg cctcgggggt tgcggtgatg aacatcgaca acgggctcgg agcgggattc
     1261 ttcgcggcga tggtggtcag gcagtcgaag tagcgccaag gctcccctcg gccgcggcca
     1321 tacggagtac ccggggaaaa acggcagagc gaaagcaggg gggagcgcat tcagcgtctc
     1381 ccccctgcac gtaccggcga agtccgctgt ttaacgaaca gatttcccgg cgtagagttc
     1441 gacgatcttc aggacaaccg ccacggcctt ctccatcgac gcgacggaga tgaattcgtg
     1501 cttcccgtgg gcgttgtgcc ctccggtgaa gatattcggc gtcagcagcc cccggtagga
     1561 gagctgcgaa ccgtccgtgc cgccgcggac cgggatgatc ttcggctcga ttccgcagcg
     1621 ccgcatcgcc tccatcgccg tctcgacgat gtgcatctgc ggcttgatct tgtcgagcat
     1681 gttgtagtac tggtccgtca ttgtcagctc cgccctgcct tcgccgtact tcttgttgat
     1741 ggccgtcacg gccgcttcga cggccttctt gcgctcggcg aatttctccg cggagtgatc
     1801 acggatgata tagttcatct ccgtagtctc tacgtttccg gtgaatccga gaaggtggaa
     1861 gaacccctcg tactcctcgg tgtgcgcggg cgtttcattg gcggggaaca tggccgcaag
     1921 ctccatgccg aggaggatgg agttcaccat cttgtccttc gacttgccgg ggtggacgct
     1981 ccgtccgtgg atgacgatct tcgcctttgc cgcgttgaag gtctcgtagc tgatctcgcc
     2041 gaccgcgccg ccgtcgacgg tgtaagcaaa gtccgcgccg aacttcttca ggtcgagcag
     2101 gctcgcgccg tgtccgatct cctcgtcggg agcgaaggca accttgacgt cgcccctggg
     2161 gagttccggg cgggcgacca taatttcgag ggccgccata atctccgcga tacccgcctt
     2221 gtcgtcggcg ccgagcagcg tcgtcccgtc ggtgacgacg agcgtctccc ccttatagag
     2281 cagcatttcc gggaacgtcg acggcgacag ggtaacgcca tcggcttcgt tgaggatgat
     2341 ctcgccgccg tcgtagttct ctacgaggcg gggacgcact gtgtcgtcgg tcacttcgag
     2401 cgccgtatcc aggtgcgcca tgaacgcaat gaccggggct ccctttgtgt ttccggggag
     2461 ggtcgctgtc acataggcgt gctcgtcgac cacgacgtct gcaagcccca gggacttcag
     2521 ctcttcaccg agctcgcggg caagcgtcat ctgccccggc gtgctcggaa ccttcccggc
     2581 gtcatgcgcc gcctggctgg gaatccggat atacttcaag aagcggtcaa gtactgaagg
     2641 catagttcta aactcctttg cgatccgtga cgaggtaggc tgtaagaagg tcgcagcacg
     2701 ccttcacggc ttgtattgtt gtacgttcgt acgaatgcga cgcgtccact ccggggccga
     2761 aacaggcaaa gtccgcgtca aaacctgcga gggccgcaac gctcgcgtcc gatccgtagc
     2821 ggaaatgcgt ttccacacgg aaaggaatgg accgctccgc ggcaaggtcc gcaagtaatt
     2881 tccggaactc gaaaccgtac ggcgtccggc tgtctctggc gacgatagtg accgcatctt
     2941 ctctggagtt agcgggagga gccacgatgc cgacgtctac ggccagatgc tcctcgacat
     3001 ccttgggcat tgcggaaatg ccgtgcccga cctcctcata ctgcgaaatg tacaggacgg
     3061 tcgtccgcgc aggaaggcgt ttcgactcca caagcgcctt gagggcgccg aagatgcacg
     3121 ccacgcagag cttgtcgtcg agga
//
LOCUS       DAOPKU010000351         3141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_34795_length_3141_cov_4.258263, whole genome shotgun sequence.
ACCESSION   DAOPKU010000351 DAOPKU010000000
VERSION     DAOPKU010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34795_length_3141_cov_4.258263"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PK849_10570"
     CDS             <1..958
                     /locus_tag="PK849_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPE66609.1"
                     /translation="ESLLRKLLKTENAVTIQSGEAMSVLWGALKSTLLPGDRLLAVSN
                     GVFGRGFGEMGMMLGMEVRIVEAPDGEFPDLTTIRTAAAEFRPRMITAVHCETPSGIL
                     NPVAGVGEIARETGALFCVDYVASAGGADVRTDEWGIDIGLLGSQKALSLLPDLSMVA
                     VSDQAWEAAERTNYQGYDALLPWRDGPKNRYLPYTHNWHALYALSLSLRKLFEEGLDR
                     SFARHEEVARYCRKRLSAMSVALYPKSEALCSPTVTAALVPQGWTWSELNGALRSRGM
                     AVGGSYGPLAGKVFRIGHMGSQADMKLVEEGMDVLENVLKSC"
     gene            complement(1131..1979)
                     /locus_tag="PK849_10575"
     CDS             complement(1131..1979)
                     /locus_tag="PK849_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66610.1"
                     /translation="MFRKSVFVVCVLGLFVSGVAIASAADLSGKWNCDDSGQYYLRQI
                     GNTLYWYGERSAANPAWSNIFQGNIQGERVNGNWVDVPKGQASSRGVLQLQIKDNGNV
                     LEATHKTGGFGGSRWVRVGYVPGTPDVPVVREDCVGFNPNTAEVKKFGARWKIVDGNH
                     AMFDFENAENEARMALRIIKHYRMNQSCFVGRPDPSFSYLLVGGHAPSGNFGGEDCVQ
                     FNPNTIEVKQIGGRWKIVDGSHWVFDFGNKEDEARLAFAIIKKYGFTRSCFVGRPNPS
                     FQYLRK"
     gene            2384..>3141
                     /locus_tag="PK849_10580"
     CDS             2384..>3141
                     /locus_tag="PK849_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66611.1"
                     /translation="MILRILLLAALLLLPRPAPAAETLNVYTIWSERYATAVFDEFTL
                     ETGIAVNFLRFPSGEVLARLIAEKGNPQVDVFFGGIADAFVAGKKEEIFEQYVPGGAE
                     TIPAAFRDPEGYWTGVAMNPICFMFNRPFLEKNALEPPASWQDLLDPVYRNGLIMADA
                     RTSGTAVSRLFSLVLAMGENEAFAYQKKLDENMQQYTKSGAGGALSIGRGQAAGGVFF
                     LVDALEMQQTGYDIVINYPREGVVYGVEAMGLVKG"
BASE COUNT          675 a          916 c          900 g          650 t
ORIGIN      
        1 cgagtcgctg cttcgaaaac ttctgaagac ggaaaacgcc gtgaccatcc agagcggaga
       61 ggcgatgtcc gtgctctggg gcgctctgaa gagcaccctg ctgcccggcg acaggctgct
      121 tgccgtctcg aacggggttt tcggcagggg attcggagag atggggatga tgctcggcat
      181 ggaagtgcgc atcgtcgagg ctccggacgg ggaattcccg gatctcacaa cgatacggac
      241 ggcggcggcc gaattccgtc ccaggatgat caccgccgtc cactgcgaaa cgccgagcgg
      301 tattctgaac ccggtggcgg gcgtcggcga aatagcgagg gagaccggcg ccctcttctg
      361 cgtcgactac gtggcgagcg cgggaggcgc ggacgtccgc accgatgaat ggggaatcga
      421 cattgggctt ctcgggagcc agaaggcgct ctcgctgctg cccgatcttt cgatggtcgc
      481 ggtgagcgat caggcatggg aggctgcgga gcggacgaac tatcaggggt acgacgcgct
      541 gcttccgtgg agagacggtc cgaaaaaccg ctaccttccc tacacccaca actggcacgc
      601 gctgtacgcg ctctcgctct cgctccggaa gctattcgag gaggggctgg atcgctcctt
      661 cgcgcggcat gaagaggttg cccggtactg ccgcaagcgc ctttccgcga tgagcgtcgc
      721 gctctacccg aagagcgagg cgctctgctc ccccaccgtc accgcggcgc tcgtaccgca
      781 agggtggacg tggagcgaac tgaacggcgc gctcaggagc agaggaatgg cggtcggcgg
      841 cagctacgga ccgctcgccg gcaaggtctt ccgcatagga cacatgggga gccaagcgga
      901 catgaagctg gtggaagaag gcatggatgt ccttgaaaac gtgctgaaaa gctgttgaga
      961 tcgtaagcag atagagcaga atcgtcttgt tctttttctc aacggagcct tcctcttttt
     1021 ttaagaggaa agctccgttg agaagcacaa tgttttctct gctgccgatc cttgagcaaa
     1081 aacactgttc tcagtttcga atgcaggacg tcctcccggg aaatgtgtta ctacttcctc
     1141 agatactgga aacttggatt cggccttccc acaaagcacg acctcgtgaa tccgtacttt
     1201 ttgatgatgg cgaaagccag gcgggcttcg tcctccttgt ttccgaaatc gaacacccag
     1261 tggctcccgt cgacgatctt ccagcgaccg ccgatctgct tcacttctat cgtattcggg
     1321 ttgaactgaa cgcaatcctc accgccgaaa ttgccggacg gggcatgtcc tccgacgagc
     1381 aggtagctga acgagggatc cggacgcccg acgaagcagg actggttcat cctgtaatgc
     1441 ttgatgatcc gcagcgccat gcgcgcctcg ttctcggcat tttcaaagtc gaacatcgcg
     1501 tggtttccgt ccacgatctt ccagcgcgcg ccgaatttct tcacctcggc ggtattgggg
     1561 ttgaaaccga cgcaatcctc gcggacaaca ggcacatccg gcgtgccggg gacgtacccg
     1621 acgcgcaccc agcgggaacc gccgaatcct ccggtcttat gtgtggcttc cagcacgttg
     1681 ccgttatctt tgatctgtag ctgaaggaca ccccggctgg aggcctgccc cttgggcacg
     1741 tccacccagt tgccgttgac tcgctctccc tggatattcc cctggaagat gttcgaccag
     1801 gcgggatttg cggcgcttcg ctccccgtac cagtagagcg tgttccctat ctgccgaaga
     1861 tagtactgcc cgctgtcgtc gcagttccac ttgccggaaa gatccgccgc ggatgcaatc
     1921 gcaacgcccg acacaaagag cccaagaacg cacacaacga aaacactctt tcgaaacatg
     1981 tttctcctcc tctccgcaca acaggatacg taatctctcc tcacaacagg gtttgataag
     2041 gcaaagcaaa gcgaagcgaa cagaaagagc gacttcaaag cgatcactaa aagtaattgc
     2101 ctgtgcgcta tcaaaaacag accaagcaga atctcaagag tatcccccta agcccgcgcc
     2161 gtatccacat tgacctcacc ttttctacca ccttccttaa atgtgacaat agtaaatttt
     2221 catgatatta ctcatatttc cacacttgtc aatcctcctt tttctctgaa acgacgccgg
     2281 acagagccag aaaccccacc ttttccgtgg caactggata taggaccatt caatgaaagg
     2341 gagtaggata catcttaaga aaacgtttag ggggatgatc ttcatgatac tgcgcatact
     2401 gcttcttgcc gcattactgc ttcttccgcg gcctgctccg gctgcggaga cgctgaacgt
     2461 ctacacaatc tggtccgagc gctatgcgac ggcggtcttc gacgagttta ccctcgagac
     2521 cgggatcgct gtcaactttt tgcgctttcc ttccggcgag gtattggcgc gcctgatcgc
     2581 cgagaaggga aatccgcagg tcgacgtctt tttcggcggc atcgccgacg ccttcgtcgc
     2641 cgggaagaag gaagaaatct tcgaacagta cgttcccgga ggggcggaaa ccattccagc
     2701 cgctttccgc gaccctgagg gatactggac gggcgtggcg atgaatccga tctgcttcat
     2761 gttcaaccgc ccgttccttg agaaaaatgc tcttgagccg cccgcatcgt ggcaggatct
     2821 gctggacccc gtctatcgca acggactgat catggcggac gcgaggacat ccgggacggc
     2881 ggtctctcgc ctcttcagcc ttgtgctagc gatgggagag aacgaagcct tcgcctacca
     2941 gaagaagctg gacgaaaaca tgcagcagta cacgaagagc ggcgcgggcg gcgcgctttc
     3001 tatcggaaga ggccaggcgg cgggaggcgt ttttttcctc gtggacgcgc ttgaaatgca
     3061 gcagaccggg tacgacatcg tcatcaacta tccgcgcgag ggcgtcgtct acggggtgga
     3121 agccatgggt ctggtgaagg g
//
LOCUS       DAOPKU010000352         3130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_34982_length_3130_cov_5.363577, whole genome shotgun sequence.
ACCESSION   DAOPKU010000352 DAOPKU010000000
VERSION     DAOPKU010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34982_length_3130_cov_5.363577"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1006
                     /gene="garR"
                     /locus_tag="PK849_10585"
     CDS             59..1006
                     /gene="garR"
                     /locus_tag="PK849_10585"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HPE66612.1"
                     /translation="MRRRRKHCSKSKRYSYMEAILMSTIGFIGLGIMGKPMAKNLLAG
                     GLSLVVYDVVSAAVDEVVAAGAAKGTSPKDVAEKSDIVVTMLPNSPHVREVVCGKNGI
                     LEGTKKGQIVVDMSSIAPLASRELGAMLAEKGAEMLDAPVSGGQEKAEKGTLAIMVGG
                     SEASYEKVKDILALMGKSTLVGELGAGQTTKLVNQTIVAINIAAVAEGMAFAKKAGVD
                     PEKVFQAIRGGLAGSQCMEDKAPRMFEGRYNPGFRINLHIKDLTNVLETSRSLHMSMP
                     LAAQLMEMFQNLAGEGHETLDHGALGLFYEKLNSLSLKK"
     gene            1141..2196
                     /locus_tag="PK849_10590"
     CDS             1141..2196
                     /locus_tag="PK849_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HPE66613.1"
                     /translation="MKKFVSMVAIALVIVFVIAGGAVAAEKVWEKGTPTVIVGFGAGG
                     GTDTSVRPVVALMSKYLNETINVANMPGAASAVAAEHVLGLPADGYTMFATGSGCFGG
                     FKVKETSNSGWEEWSSWHPFQGPAAILVAANGELKTFQDVIDALKTGKINMGASGFGN
                     GPHVIMQAIAEVAGIKEINYVTFDGDRSIATAVLAGEIEAGIVTFSSAIDMAQAGTIK
                     AVVVMTREPMKITDTITIDPIVKVLPGSDKIPDLGETWPICIRRDAPQEIKDKITEAF
                     LWAIKQPEIEEYTKKKGLNIVGLTGEDADRFISLQISGYAWVLHNSGMTEKSPEEFGI
                     PKLADWNWETEKKKLTK"
     gene            2198..2686
                     /locus_tag="PK849_10595"
     CDS             2198..2686
                     /locus_tag="PK849_10595"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HPE66614.1"
                     /translation="MAQKVYKGSGADFITAVCLVLFGAAFSIAALRMRVFNNSLLVSP
                     GLFPLILGAVFILLGLLLLRSAAKRGGWAQASHVLGKEHLHTFATSPRVRKAVVLLLL
                     IVAYVAAVANFPFLWATVGYLLVTFIYLKAMKLHWILLVSFLAAYAITFAFRDLFRIP
                     MP"
     gene            2702..>3130
                     /locus_tag="PK849_10600"
     CDS             2702..>3130
                     /locus_tag="PK849_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPE66615.1"
                     /translation="MEILHLLADEAVQVGSSLLGALNPTILALIFGGSLLGLLVGAIP
                     GLTATMALALLINLSYGMPLENAVAFLLAVYIGAVSGGLNAAIMINIPGTPSAAATCL
                     DGFPLAKEGKGGLAIGTGLIASFVGTIFSILVMIFLSPLVY"
BASE COUNT          657 a          959 c          930 g          584 t
ORIGIN      
        1 ccgaacgtca tgtgctacga ggagctgctg gagtgctacg gtcttaccaa agaggcagtt
       61 gcgaaggcgg cggaagcact gctcaaaaag taaaagatat tcatatatgg aggcgattct
      121 aatgagcaca atcggattca tcgggctcgg catcatgggg aagcccatgg cgaaaaatct
      181 tctggccgga gggctgtcgc tcgtcgttta cgacgtggtt tccgccgcgg tggatgaagt
      241 cgtcgcggcg ggcgccgcga aggggacgtc accgaaagac gtcgcggaaa agtcggacat
      301 cgtcgttacc atgctgccga actccccgca cgtccgcgag gtcgtctgcg gcaagaacgg
      361 catcctggaa ggaacgaaga aagggcagat cgtcgtcgac atgagctcca tcgcgccgct
      421 tgcgagccgc gaactgggag ccatgctcgc cgaaaaggga gcggagatgc tcgacgcccc
      481 ggtcagcgga gggcaggaga aggccgagaa gggaaccctg gccatcatgg tggggggctc
      541 cgaagcctct tacgagaagg tgaaggacat cctcgcgctt atgggcaaga gcacgctcgt
      601 cggcgaactc ggcgcgggac agacgacgaa actcgtcaac cagaccatcg tggcgatcaa
      661 catcgcggcg gtggccgagg ggatggcctt cgcgaagaag gccggagtgg acccggaaaa
      721 ggtcttccag gcaatccgcg gcggccttgc cggcagccag tgcatggagg acaaggcgcc
      781 ccgcatgttc gaagggcgct acaatccggg cttccgcatc aacctgcata tcaaggacct
      841 caccaacgtc ctcgaaacca gccgttcgct gcacatgtcg atgccgcttg cggcccagct
      901 catggagatg ttccagaacc tcgccggaga agggcacgag acgctggacc acggcgccct
      961 cggtctcttc tacgagaagc tgaacagcct gtcgctgaaa aaataaccgt ccgcgcacgc
     1021 ggacagacta tgctaggggg gatgcgccgg gaaaaattgt gacaagccag tcgcgcaccg
     1081 ttcgaaaagc ttccggaaca cgagcaacac aacctttcat atcaaaaagg agtggtcgtt
     1141 gtgaagaagt tcgtttcaat ggttgcaatc gcacttgtca tcgtcttcgt catcgcgggc
     1201 ggcgcagtcg ccgcggagaa agtatgggaa aagggaacgc ccaccgtcat cgtcggcttc
     1261 ggcgcaggcg gcggcacgga cacatccgtc cgtccggtgg tcgccctgat gtcgaagtat
     1321 ctgaacgaaa cgatcaacgt cgccaacatg cccggcgcgg cgagcgcggt tgccgcggag
     1381 cacgtcctcg gccttcccgc cgacggatac acgatgttcg ccaccggttc gggctgcttc
     1441 ggcggcttca aggtgaaaga gacctccaac tcagggtggg aggagtggag ctcctggcat
     1501 cccttccagg gccccgcagc catcctcgtc gcggcgaacg gcgagctgaa gaccttccag
     1561 gacgtcatcg acgccctgaa gaccggcaag atcaacatgg gcgcttccgg cttcggcaac
     1621 ggccctcacg tgatcatgca ggcgatcgcc gaagtggcgg gtatcaaaga gattaactac
     1681 gtcaccttcg acggcgaccg gagcatcgcc acggcggtcc tcgccggcga gatcgaggcc
     1741 ggcatcgtga ccttctcctc cgccatcgac atggcgcagg caggaaccat caaggcggtc
     1801 gtcgtgatga cgagagagcc gatgaagatc accgatacga tcaccatcga cccgatcgtc
     1861 aaggtgctcc ccggaagcga caagattccc gacctcggcg agacgtggcc catctgcatc
     1921 cgccgcgacg cccctcagga gatcaaggat aaaattaccg aagccttcct ctgggccatc
     1981 aaacagcccg aaatcgagga gtacacgaag aagaaaggcc tcaatatcgt cggcctcacc
     2041 ggcgaagacg cggaccgctt catttcgctc cagatctccg gatacgcctg ggtgcttcat
     2101 aattcgggaa tgaccgagaa atcgcccgag gaattcggca ttcccaagct ggccgactgg
     2161 aactgggaga ccgaaaagaa gaagctgacg aagtagcatg gcgcaaaaag tttacaaggg
     2221 atccggggcc gatttcatta cggccgtttg cctggtgctc ttcggggcgg cgttctccat
     2281 cgccgccctg aggatgcgcg tgttcaacaa ctccctcctc gtttcgccgg ggcttttccc
     2341 tctgatactc ggagcggtct ttatcctcct cgggcttctc cttctccgct ccgcagccaa
     2401 acgaggaggg tgggcacagg cctcgcacgt tctgggaaag gaacaccttc atacgttcgc
     2461 gacgtcgccg agagtccgca aagcggtcgt ccttcttctc ctcatcgtcg cgtacgtggc
     2521 cgcagtggcg aactttccct ttctctgggc tactgtcggg tacctcctgg tcaccttcat
     2581 ctatctcaag gcgatgaagc tgcactggat actgctggtc tcctttctgg cggcctacgc
     2641 catcactttc gcttttcggg atctcttccg gatcccgatg ccgtaaagga ggcacggata
     2701 tatggagata ctacatcttc tcgctgacga ggccgttcag gttggaagtt ccctgctggg
     2761 ggcgctcaac ccgaccattc tcgctctgat cttcggcggc agcctcttgg gacttctcgt
     2821 aggcgcaatc cccggcctca ccgcaacaat ggcgctcgcc ctgcttatca acctctccta
     2881 cggcatgccg ctggagaacg ccgttgcgtt tcttctcgca gtatatatcg gcgccgtctc
     2941 cggaggactc aacgccgcga tcatgatcaa cattccggga accccctccg ccgccgccac
     3001 ctgtctcgac ggcttcccgc tggcgaagga gggaaaggga gggctcgcca tcggcaccgg
     3061 cctgatcgcc tccttcgtcg gcaccatctt cagtattttg gtgatgatct tcctctcgcc
     3121 gctcgtctac
//
LOCUS       DAOPKU010000353         3123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35077_length_3123_cov_4.917862, whole genome shotgun sequence.
ACCESSION   DAOPKU010000353 DAOPKU010000000
VERSION     DAOPKU010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35077_length_3123_cov_4.917862"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PK849_10605"
     CDS             complement(<1..1378)
                     /locus_tag="PK849_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE66616.1"
                     /translation="MKLYGKILAPMLLLFFLGIATVSFTGYALSLRVLTEASQQKVLQ
                     AADIAEAMQRVGVVSSLIALGVVLVAGIVMLLVVRSIVKPLRDVVRVLVRRSELDFTV
                     NPADLWMIKRSKAKDEIAEMIQALILLRVKVEEMLRPLLTRSEAFSKSAQSLAELSEE
                     TVASMQEVKSSIETVFSLSEENAEAIRRTESGVGDVASSAEIAAASAQEGAKSASATS
                     ETSRLAVEKVEQVIGKIRTVETRTGESARSMGSVSASVESISRFVNTIKSIADQTNLL
                     ALNAAIEAARAGEAGRGFAVVADEVRKLAEESNHAAKEVEVLIGSLQNGTKEAMDVTN
                     ETGGVMKETVAAAEEAQRDLEDALERIGRMDGLMRNLADASGKQEAASREMTASIETA
                     SSGIASLTATFAGIRSAAEDTAGASERVAREAADLASGAGEVRSLVEAELPLRRALDI
                     YGALQGALS"
     gene            complement(1497..2294)
                     /locus_tag="PK849_10610"
     CDS             complement(1497..2294)
                     /locus_tag="PK849_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HPE66617.1"
                     /translation="MKLRVGVLQLDIAIGDKQRNCQNIEDWMASFYVPSELPTAVVVP
                     EMWNTGYALSRAKELADPEGAEAAAFLGELARKYGVWFVGGSVLASTKKGYANRAQAI
                     NPEGKLVAQYDKAHLIRLMEEEKYFTGGRKACLFDLEGAKAGCVICYDIRFCEWIRTY
                     ALKGAEVLFVSSQWPSARADHWRTLLRARAIENQMYVVACNRCGTSGDTHFGGGSMIL
                     GPKGEIYFEGGGGEEAGFATIDLDEVAETRNFLTVFNDRVPEIYSVK"
     gene            2604..2975
                     /locus_tag="PK849_10615"
     CDS             2604..2975
                     /locus_tag="PK849_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66618.1"
                     /translation="MAYITCEVCGTYIDHNYNTTKIIGRYNSMALLCEWHHAVVSALI
                     ARHGTVNWRALGRATSQVQEEKPNVLTEPERWLDYDSVARLKELYEEEIVRDPTDWPP
                     YESPNAKRAEEEEASDRYLYR"
BASE COUNT          576 a         1032 c          912 g          603 t
ORIGIN      
        1 cgctcaaggc gccctgcagc gctccataga tatccagagc ccgcctgagc ggaagttccg
       61 cctccacaag cgaccggacc tccccggcgc ccgaggccag atcggcggct tcgcgcgcaa
      121 cccgttccga cgcgccggcc gtgtcctcgg cggcggagcg tatgccggcg aacgtcgccg
      181 tgagcgaggc aatcccgctg ctcgccgtct ctatacttgc ggtcatctcc ctggacgcgg
      241 cctcctgttt tccagacgcg tcggcaagat tgcgcatcag cccgtccatc cggccgattc
      301 gttcgagcgc gtcctcaaga tcccgctgcg cctcttccgc tgcggcgacc gtctccttca
      361 tgacgccccc cgtctcgttc gtgacgtcca tcgcctcctt ggtgccgttc tggagcgagc
      421 cgatcagcac ctcgacctcc ttggcggcgt ggttggactc ctcggcgagc tttctgacct
      481 cgtcggccac gacggcgaat ccccgcccgg cctcgcctgc gcgcgcagcc tcgatggccg
      541 cgttgagcgc gagcaggttc gtctggtcgg cgatggactt gatggtgttc acaaagcggc
      601 tgatcgactc caccgaggcg gagacgctcc ccatgctccg ggccgactcc ccggttctgg
      661 tctccacagt gcggatcttg ccgatgacct gctcgacctt ctccaccgca agacggctgg
      721 tctccgatgt cgcggaggcg cttttcgcac cctcctgggc ggaagccgcg gcgatttcgg
      781 ccgaggaggc gacgtcgccg acgcccgact cggtccggcg gatagcttcc gcgttctcct
      841 ccgagagaga gaatactgtc tctatcgagc ttttgacctc ctgcatggag gcgaccgtct
      901 cctcggagag ctccgcaagg gattgcgcgc tcttcgagaa ggcctccgaa cgggtgagga
      961 gggggcgcag catctcttcc actttcaccc gcagcaggat gagcgcctgg atcatctccg
     1021 cgatctcgtc cttcgccttc gaccgcttga tcatccagag gtcggcggga ttcactgtga
     1081 agtcgagttc gctgcggcgg accagcacgc ggacgacgtc gcgcaggggt ttgacgatcg
     1141 accggacgac gagcagcatg acgattccgg cgaccagaac cactccgagc gcgatcagcg
     1201 acgagacgac gcccacacgt tgcattgctt cggcgatgtc ggcggcctga aggacttttt
     1261 gttgagacgc ctcggtcagc acgcgaagcg aaagcgcgta ccccgtaaag gagaccgtcg
     1321 caattcccag gaagaacagc aggagcatcg gcgccagaat ctttccgtac aacttcatac
     1381 acaaacccct ccctgataaa gacttgtgaa tccgcgcaac aacaatcgtg ccgtttccgg
     1441 tcgcgtcggt ctcgaacgca ccggtacgcg caccaagccg gagaacggtg ttttttctat
     1501 ttcacagagt atatttcggg aaccctgtcg ttgaagaccg tgaggaaatt cctggtttcg
     1561 gcgacttcgt cgagatcgat ggtggcgaat cccgcctctt cgccgccgcc gccctcgaag
     1621 tagatctctc ccttcgggcc gaggatcatg gagccgccgc cgaaatgcgt atcgccggag
     1681 gttccgcagc ggttgcaggc caccacgtac atctggtttt cgatggccct ggcgcggagc
     1741 agcgtccgcc agtggtccgc cctcgcggaa ggccactgcg aactcacgaa gagcacctcg
     1801 gcccccttca gcgcgtacgt gcggatccac tcgcaaaagc ggatgtcgta gcagataacg
     1861 cacccggcct tcgcgccttc caggtcgaag agacaggcct tccggcctcc cgtgaagtac
     1921 ttctcctcct ccatcaggcg gatgaggtgc gccttgtcgt actgcgccac gagcttcccc
     1981 tccgggttga tcgcctgcgc tctgttcgcg taccccttct tcgtcgatgc gagcaccgaa
     2041 ccgccgacga accagacgcc gtactttcgg gccagttcgc cgagaaaggc cgccgcctcc
     2101 gctccttcgg ggtcggccag ctccttcgcc cgcgagagcg cgtaccccgt gttccacatc
     2161 tcgggcacga cgacggcagt cggcagctcc gaaggaacgt agaacgaggc catccagtcc
     2221 tcgatatttt ggcagttccg ctgtttgtcg ccgatggcaa tgtcaagctg cagcacaccc
     2281 actcgaagtt tcatccgcta cctcctcgat ttcggccggg aacgcccctg accgtatagt
     2341 aaagtcgatg ctgtccattt ttgaaaaata ctgtgaatac gccccgatat gtttccgctc
     2401 ttatcctatg aatagtccat gaaattcttc ttgtcaagag gaaattttct gcggaggcca
     2461 aaaaacgctt gtgaccggcg attttcggtg ctacactgcc attgttcggg acaaagcccg
     2521 gcgcggcgcg tcgagccgcc ggactgcggc gaagcgcacc ggggcgggag tacatacacg
     2581 gcgtacatcc ggaggtgtaa aaaatggcgt atatcacctg cgaggtctgc ggcacgtaca
     2641 tcgaccacaa ctacaacacg accaagatca tcgggcgcta caactccatg gcgctgctct
     2701 gcgaatggca ccatgcagtc gtctcggcgc tgatcgcccg gcacggtacg gtcaactggc
     2761 gggcgctggg ccgcgcgacc agtcaggtcc aggaagagaa gccgaacgtg ctgacggagc
     2821 cggagcgctg gctagactat gattctgttg cgcgtctgaa ggaactctac gaggaagaga
     2881 tcgtccggga tccgacggac tggcctccct acgagtctcc gaatgcgaag cgggcggaag
     2941 aggaagaggc ctccgaccgc tacctctacc ggtgacggga atgccggggg aagagatgcc
     3001 ttttcgctgg cccgtcttcg aagaagctgt gcccatcgat tccgtctcct cctggaccgc
     3061 cgtacaggag agctatgacc agcgcaacga cgactgctac tacatcgtta ccctcctcaa
     3121 aga
//
LOCUS       DAOPKU010000354         3120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35117_length_3120_cov_4.699511, whole genome shotgun sequence.
ACCESSION   DAOPKU010000354 DAOPKU010000000
VERSION     DAOPKU010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35117_length_3120_cov_4.699511"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            876..1631
                     /locus_tag="PK849_10620"
     CDS             876..1631
                     /locus_tag="PK849_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPE66619.1"
                     /translation="MELVWYHWAGIVLVGVMQGFLNTVAGGGTLLVLPALTFLGMDLA
                     VANGTNRIAILLQSVSGAASFKQQKALSFKVALPLAAAATLGALVGTFVVVQVDRKSL
                     NLVIAALITLMALLLVFKPKMWEEVKEQHWPKWGVYLAFFAVGIYGGFIQAGVGFFLS
                     WALVVAAGMDLVSGNAVKTIIIGCYTTISLAIFYMNGLVNVPVGLVLAIGSMAGAVLG
                     ARFTVMKGNKWVRYVLAFVVVISAVKMVMDAIG"
     gene            complement(1693..2136)
                     /locus_tag="PK849_10625"
     CDS             complement(1693..2136)
                     /locus_tag="PK849_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66620.1"
                     /translation="MNAKESARQFLREVWRLIKEDTVPSMQEYLNLPEWDKIQLAGMG
                     MCLGDIYKAFEQSLRCILENIEGKKIEKNESWHSNLLRLSNEMELIPTDIRDTVQGML
                     KYRHHLHHGYGVKLNERLIRKYAPEAIDAFDAFTKHIISRYFDIL"
     gene            complement(2099..2410)
                     /locus_tag="PK849_10630"
     CDS             complement(2099..2410)
                     /locus_tag="PK849_10630"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPE66621.1"
                     /translation="MLSQKSLEFIKEIGEKHGVQRIVLFGSCLHLPEAEAGDIDLAVG
                     GLDAETFVEMWNDLTWASELHMKNVDLVRMEDALPVMVMVRAEGVTIYECERERATVS
                     A"
     gene            complement(2481..>3120)
                     /locus_tag="PK849_10635"
     CDS             complement(2481..>3120)
                     /locus_tag="PK849_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66622.1"
                     /translation="LQWVDEALYYYTNYGVDEVLNINPGTVFLGYLLYRMGVDVRFKI
                     SVFMGNDNPYAVLWTLLAARMFARDDGSTPLAGFNLSNSIDDAHIAKAAAIRRDLGLE
                     DQVRIEHHITETWKSIVRQPYNRREGLLKLAKTVPNISAKHEGGNPEDEQTLEHPSDI
                     LDYFREKKEVESSGEMDALTLNYLMKHKAVNSTAEALTKAGLSFVAAGAHRL"
BASE COUNT          568 a          920 c          930 g          702 t
ORIGIN      
        1 gacacctgtg ccgtctcctt tgccgactcc aacgtcaacg cccgatccga ctccaagtcc
       61 ggtacccacg actacgcccg agccgactcc aacagctact cccgtcccat ctcctacgcc
      121 ggaaccaagt ccgtcgccgt caccgacgcc gacttcaact cctgacccga ctcccactcc
      181 tgcgccgaca ccgacgccaa ctccaacagc aacgccgata ccgactccga catcaacgcc
      241 ggaacctgaa gagcctcccg ttcccgagga accgcttccc ctttcccctg cggacggcga
      301 gcgaggcctt tccctgaccc cgacgctgcg caccgcgcct tatttgcacc cgaaaaaatt
      361 cgcgcatacc tcgacgcgct ggcagatatc gtttcctgac ggcgcgtcct ctgcggcgca
      421 gggtgtgcat ctcgttttcg aaagtacgag cagcacggaa ctgaccgaac tccacgttcc
      481 ggaaggagta ttgcttcatg gaacggagta tgtttggagg acccgctttc gcgattcaca
      541 gaacggctgg tcggactggt cgccgtaccg acgttttttc actgcggatg ctccgccgga
      601 agacaagcct gagtctcttt ccgcttccgc cggcgggtgt agcagcgttt ccggcagggg
      661 cgcgctctta ctgctcccgc tcatggcgct tctcttgcgg cgtttatagg agcgctccgg
      721 cttcgttgag caagggtgca cgccgcgaag aaaaaacgcc ccttttcatc cgttctccga
      781 tttgatatac tgcaaaaaac atgttgtgag catgccgagc gtacgggagc ctcggaagag
      841 gctccctcta tcatttgaag aaggttgtga gaggtatgga actggtatgg taccactggg
      901 cggggatcgt cctcgtcggc gtgatgcagg gctttttgaa tacggtcgcg ggcggcggaa
      961 cgctgcttgt gcttccggcg ctcaccttcc tggggatgga cctggcggtg gcgaacggga
     1021 cgaaccgcat cgccatcctg ttgcagagcg tctccggggc ggcgagcttc aagcagcaga
     1081 aggcgctctc ctttaaagtg gcgctgccgc tggccgccgc ggcgacgctg ggcgcgctcg
     1141 ttgggacgtt cgttgtggta caggtggaca ggaagagtct gaacctcgtc atcgcggcgc
     1201 tgatcacgct gatggcgctg ctgctggtct tcaagccgaa gatgtgggag gaagtcaagg
     1261 agcagcactg gccgaagtgg ggcgtctacc ttgcgttctt cgcggtgggt atctatggcg
     1321 gcttcatcca ggcgggcgtc ggcttcttcc tttcgtgggc gctcgtggtg gcggcgggga
     1381 tggacctggt gagcggcaac gcggtgaaga cgatcatcat cggctgctac acgacgatca
     1441 gcctggcgat cttctacatg aacggcctgg tgaacgtgcc tgtgggcctg gtgctcgcga
     1501 tcgggagcat ggcgggcgcg gtgctcggcg cgcgcttcac ggtgatgaag ggaaacaagt
     1561 gggtccgcta cgtgctggct ttcgtggtgg tcatcagcgc ggtgaagatg gtaatggacg
     1621 cgatagggta gaaaagaaac aaccgataga aatgatgaag ccccggtgtc gccgcccggg
     1681 gctttgttcg attcaaagga tatcaaagta gcggctgata atatgttttg taaaggcatc
     1741 gaaggcatcg attgcctcgg gcgcgtattt tcgtataagc cgctcgttca gcttgacgcc
     1801 gtagccgtgg tgaaggtgat gcctgtactt aagcataccc tgtacggtgt cccgaatatc
     1861 tgtggggatg agctccattt cgttggaaag gcgcaacaag ttactgtgcc aggattcgtt
     1921 cttctcgatc tttttgcctt cgatattttc gagaatacac cgcaaggact gttcgaaggc
     1981 tttataaata tctccaaggc acatgcccat gccggcaagc tggattttat cccattccgg
     2041 caggttgaga tactcctgca tcgacggcac ggtgtcttct tttataagtc tccacacttc
     2101 acgcagaaac tgtcgcgcgc tctctttcgc attcatagat cgtcactcct tcggcgcgga
     2161 ccatcaccat gacgggcagg gcatcctcca tccgtacgag gtcgacgttt ttcatatgaa
     2221 gttcagaagc ccacgtgagg tcgttccaca tctcgacaaa cgtttccgcg tcgagtccgc
     2281 cgacggccag gtcgatgtcg cccgcttccg cctccggaag atgcaggcag gaaccgaaga
     2341 gcacaatgcg ttgcactccg tgtttctctc ctatttcttt gatgaattca aggctttttt
     2401 gcgagagcac aactctccct ccttacaggc gccgctgaat gattttttcg cgtccccata
     2461 tcgttcttat tctaccatct ctacagccgg tgcgcgcccg ccgcgacgaa ggagaggccc
     2521 gccttcgtca gggcctccgc tgtgctgttg acggccttgt gcttcatcag gtagttcagc
     2581 gtgagggcgt ccatctcgcc ggaggactcg acctccttct tctcgcggaa gtagtcgagg
     2641 atgtcggacg gatgttccag cgtctgttcg tcctcggggt tgcctccctc gtgcttggcg
     2701 gagatgttgg ggacggtctt cgccagcttc agcagcccct ccctgcggtt gtacggctgg
     2761 cggacgatgc tcttccacgt ctcggtgatg tggtgctcga tgcgcacctg gtcctccagg
     2821 ccgaggtctc tgcggatggc ggcggccttc gcgatgtgcg cgtcgtcgat ggagttggag
     2881 aggttgaacc cggcgagcgg cgtgctcccg tcgtcgcggg cgaacatgcg ggcggcgagc
     2941 agcgtccaga ggacggcgta ggggttgtcg ttccccatga aaacggagat cttgaaccgt
     3001 acgtccacgc ccatccggta gagcaggtac cccaggaaga ccgtgcccgg gttgatgttg
     3061 agcacctcgt ccactccgta gttcgtataa tagtagagcg cctcgtcgac ccactggagc
//
LOCUS       DAOPKU010000355         3119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35142_length_3119_cov_4.159595, whole genome shotgun sequence.
ACCESSION   DAOPKU010000355 DAOPKU010000000
VERSION     DAOPKU010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35142_length_3119_cov_4.159595"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PK849_10640"
     CDS             complement(<1..794)
                     /locus_tag="PK849_10640"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE66623.1"
                     /translation="MAATIFAVFTLLLVIRMPVSFSLAISAAVALVFAGSMDPLIVVQ
                     RMYTSVESFSLIAIPFFILSGGFMEHGGISRRLISFSSSLVGHIRGGLSMISIVAAMF
                     FAGISGSTAADTAAVGSVLVPAMEKKGYGKDLATSVVATAGAIGIIIPPSIPMIILGI
                     TGSISIGGLFLGGVFPGILMGLALMVTSSLFAKKRNLPSEVRATWTERRHAFTDSILA
                     LMTMVIIMGGILSGVFTATEASVVAAFYSFIVGRFVYGELKWSAIPG"
     gene            complement(796..1278)
                     /locus_tag="PK849_10645"
     CDS             complement(796..1278)
                     /locus_tag="PK849_10645"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE66624.1"
                     /translation="MERVDALVGRMERWMLILLLTAMVALGLLQIISRFVIKAPITWS
                     EALLMYMFVWFSFIGASLAVKELAHFEVEIFMVRLPQPLQKTVAVVVYCLIFAFALFM
                     VWKGVFLVKLNQRQLMAMMPFTMSVPYVILPLSGACMAIHSLKHVVGLIGRHSSKWSD
                     "
     gene            complement(1284..2150)
                     /locus_tag="PK849_10650"
     CDS             complement(1284..2150)
                     /locus_tag="PK849_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPE66625.1"
                     /translation="MEKKNGVLLQAPAETKESGVSVPVFSGDDAESVQRESGASIRVL
                     DRAVAVLGFMAEQKRQVGITEIAEATHLSKATVHRILSTLRDYSVVLKDESGRYQMGP
                     SVLYWADAYRKHSGLAELARPYLHTLWEIARETVHLFVFEAGEAYYIDKLDSPHPVGM
                     RSRIGARRDLYSTSGGRAILSFLPEAELKSYLDRTELRPRTRRTITDRKVLEEFLAEC
                     RKRGFCEEVEENEEGIRCVGAAVLDLRGYPVGAVSVSAPAYRFDDRASLATGERIRET
                     AMAISRELGYRG"
     gene            2428..>3119
                     /locus_tag="PK849_10655"
     CDS             2428..>3119
                     /locus_tag="PK849_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66626.1"
                     /translation="MSRSIVTRLALFMIMGMLVLGGALAFLARRGVDELSRAMESTTD
                     RILDEDIRNKVLNDEQTAEAYGQAMAGYLAWIALPPLWDYNYKNLSDYAAGMLNVPNV
                     LAAVIYKEDGSIAAGEMMIGSKNGGKDSRVFTSDIVREGQKIGSVELVLSTAHLEQLR
                     QKSEETREELFASFKANTAATEQSVMRRMFLLSAVILAALLALITFSIIKLVAPLRGM
                     TRAIRRFSETDS"
BASE COUNT          693 a          966 c          888 g          572 t
ORIGIN      
        1 ccgggaatcg cgctccattt cagctcgccg tagacaaagc gccctacgat aaacgaatag
       61 aacgccgcca ccacggacgc ctccgtcgct gtgaagacgc ccgaaaggat gccgcccatg
      121 atgatgacca tcgtcatcag ggcaagaatg gagtcggtga acgcatgccg gcgttccgtc
      181 catgtagcgc gtacttcgga aggaaggttg cgctttttgg cgaagagcga actggtgacc
      241 atcagggcga gccccatcag gatgccgggg aagacgccgc cgaggaagag cccgccgatg
      301 gagatgctcc ccgtaatgcc gaggatgatc atcgggatgc tcggggggat gatgatgccg
      361 atggcccccg ccgtcgccac cacgctggtc gccagatcct tgccgtaccc ttttttctcc
      421 atcgcgggga cgagcacgct gcccacggcg gcggtatctg ccgccgtgga gccggagatt
      481 ccggcaaaga acatggccgc cacgatgctg atcatggaga gcccgcccct gatatgcccg
      541 acgaggctcg acgagaagga gatcaggcgg cgcgagatcc cgccatgctc cataaatcct
      601 cccgagagaa tgaagaacgg gatggcgatc agggagaacg actccaccga agtgtacatt
      661 ctctgaacga cgatgagcgg gtccatgctc cccgcgaaga cgagggcaac ggccgccgag
      721 atggcaagcg aaaaggagac cggcatacgg atgacgagca gcagcgtgaa aaccgcgaaa
      781 atagtggcag ccatgttaat cactccactt cgaagaatga cggccgataa ggccgacgac
      841 gtgtttcagc gaatgaatcg ccatgcaggc gccgctcagc ggcagtatca cgtaggggac
      901 gctcatcgta aagggcatca tcgccatgag ctgcctctgg ttgagcttca ccagaaaaac
      961 ccccttccag accatgaaga gggcgaaggc gaagatcaaa cagtacacga caacggcgac
     1021 cgtcttctgc agcggctgcg gaagacggac catgaaaatc tcgacctcga agtgcgccag
     1081 ctccttcacg gcgagacttg cgccgatgaa gctgaaccag acgaacatgt acatgaggag
     1141 ggcctccgac cacgtgatcg gcgctttgat gacgaaccgg ctgatgatct ggagaagccc
     1201 cagcgccacc atcgccgtca gaagaaggat caacatccag cgttccatgc gtcctacaag
     1261 agcgtctaca cgttccatga gttttacccc ctatacccca gctcgcggga gattgccatt
     1321 gccgtctccc gtattcgttc ccctgtcgcc agagaggctc tgtcgtcgaa acggtaggcc
     1381 ggagcgctca cgctcaccgc ccccacgggg tagccccgca gatcaagcac ggcggcccct
     1441 acgcagcgaa tcccctcctc gttctcctcc acctcctcgc agaagccgcg tttgcggcat
     1501 tcagcgagaa actcttccag caccttcctg tccgtgatgg ttctcctggt tcggggacgg
     1561 agctccgtcc tgtcgaggta ggatttcagc tccgcctcgg gaaggaacga aaggattgcg
     1621 cggccgccgg acgtgctgta gaggtcccgc cgcgcgccga tgcgggaacg catgcccacg
     1681 gggtgcgggc tgtcgagctt gtcgatgtag tacgcctccc ccgcctcgaa gacgaagaga
     1741 tgcaccgttt cccgggcgat ctcccacagc gtgtgcagat acggacgcgc cagctcggcc
     1801 agccccgagt gtttccggta cgcatccgcc cagtagagga cggagggccc catctgatac
     1861 cttcccgact cgtccttcag aacgacggag tagtctcgga gcgtcgagag aatacggtgc
     1921 accgtcgctt ttgaaagatg cgtcgcctcc gctatttccg tgatacctac ctgccgtttc
     1981 tgctctgcca tgaagccgag cacggcaacc gcacggtcaa gcaccctgat ggaggctcct
     2041 gactcccgct gcacgctctc ggcgtcgtct cccgaaaaaa caggaacgct gacgccgctc
     2101 tcctttgtct ccgcaggagc ttgtaacagt acgccgttct tcttctccat aataattcca
     2161 cctgccaaaa tagcgattct ttggatgaaa caacactaag gtacgataca ggggcgagga
     2221 tgtcaactct ttttcagtgg aacttttccg ttgaatccaa gaagtcgaac aaaaagaaaa
     2281 aactttcact gatcgcttcc ctgctgtaca atgtctgaaa ccagcgctgc atctcccctg
     2341 cgcgaagatc cggcgtttgg tatcgtattc cgaaagacaa gggcaactcc cgcctttttg
     2401 cgcgcaaccg gacaggagga gtgctgtatg tccagaagta tcgtcacacg actcgccctt
     2461 tttatgatca tgggcatgct tgttcttgga ggagctctgg cgtttctcgc ccgaaggggc
     2521 gtggacgaac tctcccgcgc tatggagtcc actaccgacc gcattctgga cgaggacata
     2581 cggaataaag tgctgaacga cgagcagacc gccgaagcct acggacaggc catggcgggg
     2641 tacctcgcct ggatcgcgct ccctcccctg tgggactaca actacaagaa cttgtccgac
     2701 tacgccgcag gaatgctcaa cgtccccaac gtcctcgccg cggtcatcta caaggaagac
     2761 ggttcgatcg cggcgggcga gatgatgatc ggttcgaaga acggcgggaa agactcccgg
     2821 gtgttcacat ccgatatcgt gcgcgagggg cagaagatcg gctccgtgga gctcgtcctc
     2881 agcaccgcgc accttgagca actgcggcag aagagcgaag aaacgcgcga agagctcttc
     2941 gcctccttca aagcgaatac cgccgccacc gaacagtccg ttatgcgaag aatgtttttg
     3001 ctctcggcgg tgatcctcgc cgccctcctc gcgctgatca cattcagcat catcaagctg
     3061 gtcgcgccgc tgcgcgggat gacgcgcgcc atacgccgct tctcagagac ggactcctt
//
LOCUS       DAOPKU010000356         3119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35150_length_3119_cov_3.404700, whole genome shotgun sequence.
ACCESSION   DAOPKU010000356 DAOPKU010000000
VERSION     DAOPKU010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35150_length_3119_cov_3.404700"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PK849_10660"
     CDS             <1..375
                     /locus_tag="PK849_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPE66627.1"
                     /translation="DFRFKVAARKTAVLIAACVESGAVLGGADQAAANAFRTYGNHLG
                     LAFQMVDDILDVTSSAEELGKTPGKDATQNKKTFVSLFGLEETRRLAHNETEKGINAL
                     RTIEGDTGFFAALSENLVERTR"
     gene            378..2291
                     /gene="dxs"
                     /locus_tag="PK849_10665"
     CDS             378..2291
                     /gene="dxs"
                     /locus_tag="PK849_10665"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPE66628.1"
                     /translation="MPVLERMKHYSELKGYSKETLDTLAAELREYIVSVVLKTGGHMA
                     SSLGAVELILALLRSFDPEDDKILFDVGHQAYAYKILTGRRDRFPTLRQWGGISGFPK
                     RSESPYDHFDVGHSSTSLSAALGYAKARDVLGAGNHVVAVIGDASLLNGLAFEALNYT
                     KDSNTKVIYILNDNTMSISKRVGGFATHLAKLSSSTAYNSLKKAIKESCASLPKGQAL
                     ENVLGKLKDHIKSIVKPINIFDEMDINYWGPFDGHNIEEIENVLELAKKYDKPVLIHL
                     LTVKGKGYEKAEQEPTKYHGVPPCKAVAVPATVSRRSWSDAASSAVCALAERDKRVVC
                     LTAAMKCGSKLDTFAERFPDRFFDVGIAEGHMLTMAAGMAAGGLRPVVFIYSTFLQRA
                     MDQLVHDIAMQNLPVTIAVDRSGLIGDDGETHQGLLDISWSRSVPHLVVAAPRDEIGL
                     SALFDLGLRRNGPMMIRFPRGEAIPSLHRIRGEVFLDDLPVPEILEKGTECALLGYGK
                     TVELMLDAREIIVRDGGVPPSVVDIKCVKPMCVPVMEGLLKSHELVIVAEDGYKECGV
                     GEAIASLAEKLRPSSRTAMVLPMGIPDLFVPQGTVSEQAEYCGLTPRKVVTMYEEYQK
                     RTHRQGSGIQGVR"
     gene            2233..3060
                     /locus_tag="PK849_10670"
     CDS             2233..3060
                     /locus_tag="PK849_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPE66629.1"
                     /translation="MKSIKKERIDKVLVSRGFVETRTKAQSLLMAGKVTVDGNRVDKP
                     GALVFPDAAVEVIGTGNEWVSRGALKLLKALDVFPFDPKDRICVDIGASTGGFTQVLL
                     SRGALRVYSVDVGYGQLAWSLRSDERVVVMERTNARNLLPDNFPDIPDRAVADASFIS
                     LKLLLPAMDTILSPQGEAVVLVKPQFEVGKGKVGKGGVVRSSEDHIVVLEEILNFCLR
                     ETTFTPCGITFSPITGPMGNIEYLLYLSKSDSEPSIPHPVSIVEEAHSFFTKGRAAS"
BASE COUNT          744 a          756 c          877 g          742 t
ORIGIN      
        1 gatttccggt tcaaggttgc tgcccggaaa accgcagtcc ttattgccgc ctgcgtggaa
       61 agcggcgccg tccttggagg ggccgaccaa gcagccgcaa acgctttccg tacgtacggc
      121 aatcatttgg gtttggcctt tcagatggtc gacgatattc tcgatgtcac gtcctcggca
      181 gaagaactgg gaaaaacacc aggcaaagac gccactcaga acaaaaagac gtttgtttcc
      241 cttttcgggc ttgaagagac tcgccggtta gctcataacg agactgaaaa agggataaat
      301 gcactccgta cgatagaagg agacactgga tttttcgcgg ctctctccga aaatcttgtg
      361 gaaaggacgc ggtagatatg cctgttctgg agcgtatgaa gcactattcg gagctgaagg
      421 ggtactcaaa agagactctc gatacgctcg ccgctgaact tcgagagtat atagtctcgg
      481 tagttttaaa aaccgggggt catatggcct cttcgctggg ggcggttgag ctcattcttg
      541 cgctgctgcg ttcctttgat cctgaagacg ataaaatact cttcgatgtc ggacatcagg
      601 cgtacgcgta taaaattctt actggccgca gggaccgttt tccgacgctg cggcagtggg
      661 gcggtatcag cggttttccg aaacgttccg aaagtccgta cgaccatttt gacgtggggc
      721 atagcagcac atcgctttcc gcagcgcttg gttatgcgaa agcgagagat gtcctgggcg
      781 cgggaaacca tgtcgtagcg gtgataggag acgcatcgct cctgaacggg ctggcattcg
      841 aggcgctcaa ctacaccaag gattcgaaca cgaaagtcat ctatatcctc aatgacaata
      901 caatgtccat cagtaaaaga gtaggcggct ttgccacaca tcttgcgaaa cttagctcga
      961 gcaccgccta taattctttg aagaaggcga tcaaggagtc ctgcgcctcg ctaccgaagg
     1021 gacaggctct tgagaatgtg cttggaaagc tgaaggatca catcaaatcc atagtcaagc
     1081 cgatcaatat tttcgatgaa atggatatca actactgggg accttttgac ggacataata
     1141 ttgaagaaat agaaaatgtc ctggagcttg cgaaaaaata tgataaacca gtgctcattc
     1201 atcttctaac agtgaaggga aaagggtatg aaaaagcgga gcaggaaccc acgaagtatc
     1261 acggcgttcc tccttgcaaa gctgttgccg ttccggcaac cgtttctcga agatcatgga
     1321 gcgacgctgc ttcaagcgca gtgtgcgctc ttgcggagcg cgacaagcgg gtggtctgcc
     1381 ttactgctgc catgaagtgc ggcagtaagc ttgacacgtt cgcggagcgt ttccctgatc
     1441 gtttctttga tgtaggcata gccgaggggc acatgctcac tatggcggct ggtatggcgg
     1501 ctggaggatt acggccggta gtgtttatct actcgacatt cctccagcga gcaatggatc
     1561 agctcgtcca tgatatcgct atgcagaatc tcccggttac catcgcagtt gaccgttcgg
     1621 ggctcatcgg cgacgacgga gagacacatc aaggattgct cgacatttcg tggtcgcgtt
     1681 ccgtacctca ccttgtcgtg gcggctccaa gggacgagat agggctttca gcgctcttcg
     1741 atctggggct tcgtcgaaac gggccgatga tgatccgctt tcccaggggc gaggcgattc
     1801 cttcccttca caggattcgg ggcgaagttt ttctggacga ccttcctgtt ccggaaatcc
     1861 tggaaaaggg aactgagtgc gcgctcctgg ggtacggaaa gaccgtcgaa ctgatgctgg
     1921 atgcgcgcga aataattgtt cgcgacggtg gcgttccccc ttctgttgtg gacatcaaat
     1981 gtgtcaaacc catgtgcgtt ccggtaatgg aggggctctt gaaaagccat gaacttgtca
     2041 tcgttgccga agacggttac aaagagtgcg gagtaggcga ggcgatcgcg tcccttgccg
     2101 aaaaactcag gccgtcgtcc cgaacagcca tggtgcttcc gatgggcatc cccgatcttt
     2161 tcgttcccca ggggacggtt tcagaacagg ccgagtactg cggccttacc ccccgtaaag
     2221 tggtgacaat gtatgaagag tatcaaaaaa gaacgcatcg acaaggttct ggtatccagg
     2281 gggttcgttg aaaccagaac aaaagcgcaa agccttctga tggccgggaa agttacagtc
     2341 gatgggaatc gggtggacaa gccgggcgcg cttgtctttc ctgacgcggc tgtggaagtg
     2401 atcggaaccg gaaacgaatg ggtcagccgc ggcgcactca aactgctgaa agctctcgat
     2461 gtctttcctt ttgaccccaa agacagaatt tgcgtcgata tcggcgcctc aacgggaggg
     2521 ttcactcaag ttctcctttc aaggggcgct cttcgggtat attccgtcga tgtcgggtat
     2581 gggcagttgg cttggtcgct tcggagcgat gagagagtcg ttgtaatgga acgtacgaat
     2641 gccaggaatt tgcttccgga caattttcct gatatacccg acagggcggt tgctgatgcg
     2701 tctttcattt ctctgaagtt attgctgccg gctatggata caattctttc cccccaagga
     2761 gaagcggttg tccttgttaa gccgcagttt gaagtcggta aagggaaagt tgggaaggga
     2821 ggcgtggtcc gttcgagtga ggatcacatc gtcgttttgg aggagattct gaatttctgt
     2881 ttacgggaaa cgacgttcac gccatgcggt ataacatttt cacccattac cggtcctatg
     2941 gggaatatcg agtatctcct ctatctctcg aagtcagact cggagccttc gattccacac
     3001 ccagtctcga tagttgaaga agcgcatagt tttttcacga aaggacgggc ggcatcatga
     3061 acactcccaa tattggcatg cttgtcaata cgcgaaaacc cggggcgata aaaatggca
//
LOCUS       DAOPKU010000357         3116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35186_length_3116_cov_3.612872, whole genome shotgun sequence.
ACCESSION   DAOPKU010000357 DAOPKU010000000
VERSION     DAOPKU010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35186_length_3116_cov_3.612872"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2261)
                     /gene="flgK"
                     /locus_tag="PK849_10675"
     CDS             complement(<1..2261)
                     /gene="flgK"
                     /locus_tag="PK849_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPE66630.1"
                     /translation="MLNSFFGLEMGRRALDYFRRGMETAGHNISNADVEGYSRQRVEA
                     STTPPFTDPAFNRPAIPGQIGTGVKVDAIARLRDAFLDIQFREESTVKGYWEQIDRAL
                     TTLEVYVNEPGGKGFKASLDEYWTSLQELHKRPDSSAVREEIVEKTKNLTVYLDQLVQ
                     NYDEYRVAMNREVKLMVEESNALIDQIAALNVTIAQVQGTGGNPNDLMDRRDLLVEKL
                     SKFMDISVNPPCLEGDGEFKIDLGGKLLVQGDKTRHLVLVDVIGNGGFFDVQVEDNLF
                     DVVSNTNVLHAIVEQGAPEAVHSVSVQRLATETAWSVGQGDSSCPERVKPETTTEALN
                     LSGSFSIQIGAQGTRTASSIFTSNPPGVGVVLEPGDEGDEYAFRVSAHETERNINVAW
                     DNGAGQWNISVDGVLAGFSNTGSLTLNDLHSALAGFAPNVTVSVNAAGSQLGFAAMDN
                     DLLSIVDIKGNLASTLGIDNDAPVVNIDVVEEDTLETIRNKINSAYGAAAGLDRPEDW
                     LHASIELDTATNSHYLVLESNTAGEAHRINILSDEKGSLQIAKRLGFLNGADDSTSYR
                     TISRDVSFTFDGKHYLSESNAFRNARRVPVQNDYSATVMEEVSEGIRLDLKSEGQSSI
                     TVRHHIKGGAIKGVLEARDDIILNFSDVFDEMAYTLASEMNAIHYAGHGTGENAATTG
                     YAYFTPINTLYGASRNLSINRAIDEDRSLIAAASGDGTGHTLGEGDGSNALRMAQLKQ
                     TKVLQSRSSDFNT"
     gene            complement(2278..2730)
                     /gene="flgN"
                     /locus_tag="PK849_10680"
     CDS             complement(2278..2730)
                     /gene="flgN"
                     /locus_tag="PK849_10680"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HPE66631.1"
                     /translation="MASPSSLGDALTKQAEILRTLGEVTVRQREALKEGRLELLQDLF
                     REQQAAGFAAEAAEERRRRNAADLAATLGCEPRVSSICSRLSDNEAYQLKQKADDVLR
                     QVKVLQSEMYIISSLVEENQRLNGMMAAEWRRLEGMYPSSRSGVDFKG"
     gene            complement(2733..3020)
                     /gene="flgM"
                     /locus_tag="PK849_10685"
     CDS             complement(2733..3020)
                     /gene="flgM"
                     /locus_tag="PK849_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPE66632.1"
                     /translation="MIDKIPGVYGPGVMDKLNAKKGYKPSREGGKGDGVEFSSFAVAL
                     AKVSGELKKIPDVREQLVEDFKRQIAEGTYSPDMNQVARSLIGAGLLNRED"
BASE COUNT          520 a          902 c          972 g          722 t
ORIGIN      
        1 aacgtgttga agtcggagct gcggctctga agcactttcg tctgtttgag ctgggccatg
       61 cggagcgcgt tgctgccgtc tccctcgccc agcgtatgtc cggttccatc gccgcttgcc
      121 gccgcgatca ggctgcggtc ctcgtcgatc gcccggttta tggagaggtt gcggctggcg
      181 ccatacagcg tgtttattgg cgtgaaatag gcatatcctg tggtcgcggc gttctctccc
      241 gtgccgtgac cggcgtagtg gatggcgttc atctcgctcg ccagcgtgta ggccatttcg
      301 tcgaagacgt cggaaaagtt gaggatgata tcgtcccgcg cttcgagcac gcccttgatc
      361 gcgccgcctt tgatgtggtg gcggacggtg atgctcgact gtccctcgga cttgaggtcg
      421 agccgtatac cttcggaaac ctcttccatc acggttgcgg agtagtcgtt ctggacgggt
      481 acccggcggg cgttccggaa ggcgttgctc tccgagaggt agtgttttcc gtcgaaggtg
      541 aacgagacgt cccgggagat cgtccggtag ctggtgctgt cgtcggcgcc gttgaggaag
      601 ccgagccgct tcgcgatctg gaggctcccc ttttcatccg agaggatgtt gatcctgtgc
      661 gcctcgcctg ccgtgttgct ttcgagaacg agatagtggc tgttcgtggc cgtatcgagc
      721 tcgatggagg cgtgaagcca gtcttcgggg cggtcgagtc cggcggctgc accgtacgcg
      781 ctgttgatct tgttccgtat ggtctcaagc gtgtcttcct cgacgacgtc gatgttgacg
      841 acgggggcgt cgttgtcgat gcccagcgtc gaggcaagat tacccttgat gtcgacgatg
      901 gagagaaggt cgttatccat cgcggcgaaa ccgagctggc ttcccgccgc gttgaccgaa
      961 actgtcacat tgggagcgaa cccggcaagc gcggagtgca ggtcgttcag tgtgagagaa
     1021 ccagtattcg agaagcccgc gagcactccg tcgacggata tgttccattg tcctgctcca
     1081 ttgtcccacg ctacgttgat attccgctcg gtctcgtgcg cggagacgcg gaaggcgtac
     1141 tcatcgcctt catctcccgg ttcgagcaca acgccgacac ccggagggtt gctggtgaag
     1201 atggacgacg cggtgcgcgt gccctgggcg ccgatctgta tggagaatga gccgctgagg
     1261 ttgagcgcct cggtggtcgt ctccggtttt acccgctcgg ggcacgagga gtcgccctgg
     1321 ccgacggacc atgcggtttc ggtggccaga cgctggacgc tcacggagtg aacagcctct
     1381 ggcgctccct gctcgacgat ggcgtggagg acgttggtgt tggagacgac gtcgaagagg
     1441 ttgtcctcga cctgtacgtc gaagaaaccg ccgttgccga taacatcgac aaggacgagg
     1501 tggcgggtct tgtcgccctg gacgaggagc ttgcctccca aatctatctt gaactcgccg
     1561 tcgccttcga ggcacggagg attgacgctg atgtccataa acttggagag cttttccacc
     1621 agcagatcgc gccggtccat aaggtcgttc ggattgcctc ccgtaccctg aacctgcgct
     1681 atagtgacgt tcagcgcggc gatctggtcg atgagagcgt tgctctcctc caccatcagt
     1741 ttgacctcgc ggttcatggc gacgcggtat tcgtcgtagt tctgaacgag ctggtcgagg
     1801 tagacggtga gatttttcgt cttttcgacg atctcctcgc ggaccgcgga gctgtcgggg
     1861 cgcttgtgca gctcctggag gcttgtccag tattcgtcga gagaggcctt gaaccctttg
     1921 cccccgggtt cgttgacgta gacctcaagc gttgtcagcg cgcggtcgat ctgctcccag
     1981 taccctttga cggtgctctc ctcgcggaac tggatgtcga gaaaagcgtc gcggaggcgg
     2041 gcgatggcgt ccaccttgac gccggtgcct atctgtccgg gaattgcggg acggttgaag
     2101 gcggggtcgg tgaacggagg cgtcgttgaa gcctcgacac gctgccttga gtagccctcg
     2161 acatcggcgt tcgatatgtt gtgtccggcg gtttccatac cccggcggaa gtagtcgagg
     2221 gcgcggcggc ccatttcaag accgaaaaag ctgttcaaca tggggatggc agcctcctta
     2281 tcctttaaaa tcaacgccgg agcgcgagga cgggtacatt ccttcgagcc gtcgccactc
     2341 cgcggccatc atgccgttga ggcgctggtt ctcctcgacg agcgaggaga tgatatacat
     2401 ctccgactga agtaccttca cttggcgaag gacgtcgtcg gccttttgtt tgagttgata
     2461 cgcctcgtta tccgacaggc gcgagcaaat cgaggagact ctgggctcgc atccaagcgt
     2521 ggccgccaga tcggcggcat ttcgtctgcg ccgctcttcg gcggcttccg cggcgaaccc
     2581 tgccgcctgc tgctcgcgga agaggtcctg taataattcg aggcgccctt ctttgagcgc
     2641 ctctctctgc cgtacagtaa cttcaccgag cgtgcgcaga atttccgcct gcttggtcag
     2701 cgcgtctccc agagaggacg gtgaggccac ggttaatcct cccggttcag cagccccgcg
     2761 ccgataaggc ttcgcgctac ttggttcata tccggtgagt aggtcccctc ggctatttgt
     2821 cgcttaaagt cttcaacaag ctgctcgcga acgtcgggga tttttttcag ctcgccggaa
     2881 actttagcga gcgccacggc gaaggagctg aactctactc cgtcgccctt gcccccctcg
     2941 cgggaaggct tgtacccttt cttcgcgttg agcttgtcca ttacgcctgg tccgtacacg
     3001 ccgggaatct tgtcaatcat cttcatcacc ccgaaagaag aaactttttc tcttcacttt
     3061 gttatcggac gccgcgcatc ctctctttag tccggggcgc gcaggtccac gaaagt
//
LOCUS       DAOPKU010000358         3112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35245_length_3112_cov_4.888453, whole genome shotgun sequence.
ACCESSION   DAOPKU010000358 DAOPKU010000000
VERSION     DAOPKU010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35245_length_3112_cov_4.888453"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..2413
                     /locus_tag="PK849_10690"
     CDS             152..2413
                     /locus_tag="PK849_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66633.1"
                     /translation="MFFDIRGIVLFWLVVNLMLTVTNIAMYFRYRKRFEGLLFWSVHM
                     ILQVTGMTFIFLRGFIPGFVSVFVANGMLAMGALSLLVGLELFVDKRRRHGFNLAAIA
                     GYLAVMAYFYWIRPDLAVRTVATSALHAAVAFQICALLLRRVPCGLRDITRITGLVAG
                     VYAAVHFSRMCLLLSFSPQTNDLFRSGFVDSFFLACILTLEIALKLAIIMMLSRRLLE
                     TLEQTTAEAERMAVAAQTANRAKSEFLANMSHEIRTPMNGVLGMTGLLLDMRLTDEQR
                     SCAEAVRSSGEHLLGILNDILDFSKIEAGRLDLESVDFDLRAVLEEIVSILGARAGAK
                     GLQLRLSMDPGVPASLRGDPLRLRQVLNNLADNAVKFTSRGEVSISVEPEEEGKNDVA
                     LRFSVRDTGIGIPLEKQGALFDKFSQVDTSTTRKYGGTGLGLAISKKLVEMMGGEIGV
                     TSAEGRGSEFWFSARFGKASGAGRALPQKEHRDMTPLSAGQRRARLLVAEDNTTNLQV
                     ALGILKGLGLDADAASDGEEAVEAVQRHPYDLVLMDCQMPRMDGYEATRRIRSLEGDV
                     RDVPIIAMTAHAMAGDREKCLQAGMNDYVSKPISPRLFVETLRRWLPSGADALEDAPG
                     LKETVGGSCSASSGQDQEFASRVWDRSLLLERVLGDEALAEDVVAGFVSDMPERIAAL
                     EKAVAAGDFGETENLSHALKGAAGSVSAEALMRVAKEMEKKSKESDLDALRTILPAVM
                     WEYERLRSALEKK"
     gene            2503..2985
                     /locus_tag="PK849_10695"
     CDS             2503..2985
                     /locus_tag="PK849_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66634.1"
                     /translation="MTGRIVTVLVRGVLLLLLCAVSSEAQARMEKEYCNARFGFCFSY
                     PAHFEMDPPPENDDGRILRDGDGCVVTVWGGYNVLEETLAEAMTSRSADFDTVTYSAK
                     GKNWFVLSGYRGSDIVYLKLWVGPDAVNGLEMAYPSALKKEYDAVVTNIVRSFRPGDL
                     "
BASE COUNT          579 a          858 c         1020 g          655 t
ORIGIN      
        1 ccgctttgtc ccccgcttct ccgagaagac gggggatttt tttataacga atcttccgtc
       61 cgtcccgcag cgccgcttgc gagaggaacg aaagtgcact atcatgcgtt gaggagccgc
      121 gttcgcgaat cgggtacggg agggaaatcc catgtttttc gatatcagag gcattgtgct
      181 tttctggttg gttgtcaatc ttatgctcac cgtaacgaat atcgccatgt acttccgcta
      241 ccggaagcgc ttcgaaggcc tcctcttctg gtccgtgcac atgattcttc aggtgacggg
      301 catgaccttc atctttcttc gagggttcat ccccgggttc gtctccgttt ttgtcgccaa
      361 cgggatgctc gccatggggg cgctctcgct cctcgtgggg ctcgaactgt ttgtggacaa
      421 gaggcgacgg catgggttca acctcgcggc gatcgcaggc tatcttgcgg ttatggccta
      481 cttctactgg attcgtcccg atctcgcggt gcgcaccgtc gcgacgtccg ctcttcatgc
      541 cgccgtcgcg tttcagatct gcgcgttgct gctccggcgg gttccttgcg gtctgcggga
      601 catcacgcgc atcaccggac tggtggcggg cgtctacgcc gcggtccatt tttcccgaat
      661 gtgtttgttg ctctccttct cgccacagac gaacgatttg ttccgctccg gttttgtcga
      721 ttcgtttttt ctcgcctgta ttctcacgct ggaaatagcc ttgaagctcg ccatcatcat
      781 gatgctctcg cgccgccttc tcgaaaccct cgaacagacc acagcggagg ctgagcggat
      841 ggccgtggcc gctcagaccg cgaatagggc gaagagcgaa ttcctggcga atatgagcca
      901 cgagatacgg acgcccatga acggagttct ggggatgacg gggttgctgc tcgatatgcg
      961 tctgaccgac gagcagcgga gctgcgccga ggccgtgcgt tcgagcggcg agcatctcct
     1021 cggcattctc aacgatatcc tggacttctc gaagatcgag gccgggcggc ttgatctgga
     1081 gtccgtggat ttcgacctgc gcgccgtgct cgaggaaatc gtctccatcc tgggagcgcg
     1141 tgccggggcg aaaggcctgc aactgcgcct ttccatggat cccggcgtgc cggcctcgct
     1201 gcggggagac ccgctgcgac ttcgacaggt gctgaacaac ctggcggaca atgcggtcaa
     1261 gttcacttcg cggggcgagg tttccatctc cgtagagccc gaggaggaag gaaaaaacga
     1321 tgtcgcgctt cgcttttcgg tgcgcgacac ggggatcgga atccctttgg agaagcaggg
     1381 agcgctcttc gacaagttca gccaggtgga tacctcgacg acaagaaaat acggaggcac
     1441 cggccttggc ctcgctatct cgaaaaaact cgtcgagatg atgggcggag aaatcggcgt
     1501 aacaagcgcc gaaggccggg gatcggagtt ctggttttcg gcgcggttcg gcaaggcgtc
     1561 cggtgcgggg cgcgctcttc cgcaaaagga acaccgcgac atgacgccgc tctccgcggg
     1621 gcaaaggcga gcgcggcttc tcgtcgcgga ggacaacacg accaacctcc aggtcgcgct
     1681 gggcattctg aaaggcctgg gactggacgc ggacgcggcc tcggacggag aggaggctgt
     1741 cgaggcggtg caacgccatc cgtacgattt ggtgctgatg gactgccaga tgccccggat
     1801 ggatggatac gaggcgacgc ggaggattcg ctcgctggag ggcgacgttc gcgacgtccc
     1861 gatcatcgcg atgacggcgc atgcgatggc cggggacagg gagaagtgcc tgcaggcggg
     1921 aatgaacgat tacgtttcca agccgatttc tcctcgtctc ttcgtcgaaa cgcttcggcg
     1981 ctggcttcct tcgggggcag acgctctcga agatgctccg gggctcaagg aaactgtcgg
     2041 cggaagttgc tcggcttctt cggggcagga tcaggagttt gcgtcacgtg tttgggacag
     2101 gagcctcctg ctggagcggg tgctcgggga tgaagccttg gccgaagatg ttgtcgcggg
     2161 gtttgtgagc gatatgccgg agaggatcgc cgctcttgag aaggctgtgg cggccggcga
     2221 cttcggcgaa acggagaacc tgagccacgc tctgaaaggg gccgccggaa gcgtctccgc
     2281 ggaagcgctg atgcgtgtcg cgaaggagat ggagaagaaa tcgaaagaga gtgaccttga
     2341 cgctctcagg actattctgc cggctgtgat gtgggaatat gaacggttga ggtcggcgct
     2401 cgaaaagaag taggaaggga aatctccgag tcttcttcgt gcgagcggtt ccgggcgggg
     2461 acgcgacgaa actgttctcc ccgatgcgaa gggaggaaaa tcatgactgg acgcatagtt
     2521 acagtgttgg tgcgaggcgt tctgctcctt cttctctgtg ccgtctcttc ggaggcgcag
     2581 gcgcgaatgg agaaagagta ctgcaacgcg cgcttcggct tctgtttttc gtacccggcg
     2641 catttcgaga tggacccgcc tccggagaac gacgacggac ggattctgcg cgacggcgac
     2701 gggtgcgtcg tgactgtgtg gggcgggtac aacgtcctcg aagagacgct tgcggaggcg
     2761 atgacctcgc ggagcgccga tttcgacacc gtgacctaca gtgccaaggg gaagaactgg
     2821 ttcgtcctct cggggtacag agggagcgac atcgtctatc tcaaactctg ggtggggccc
     2881 gacgcggtca acggactgga gatggcgtac ccgtcggcgt tgaaaaagga gtacgacgcg
     2941 gtggtgacga acatcgtccg ttcgttccgt cccggagatt tgtaagcggt ccgcggaaac
     3001 cgcaaaaacc ggagagatgc gcgggaacgc cgcctgtgtt ttatgcctcc ccttttccgc
     3061 cctctacaaa aactccagcg ggcagaagtc cttcgagaag gtcttcgttc cg
//
LOCUS       DAOPKU010000359         3111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35260_length_3111_cov_4.818063, whole genome shotgun sequence.
ACCESSION   DAOPKU010000359 DAOPKU010000000
VERSION     DAOPKU010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35260_length_3111_cov_4.818063"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            557..>3111
                     /locus_tag="PK849_10700"
     CDS             557..>3111
                     /locus_tag="PK849_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPE66635.1"
                     /translation="MPGSVFRRPVFILGEVFIRSISQHEWMVMGMKKTKLALILLFLF
                     LCAGSAFGQPKAPSDEYVRGDVLVVLEAPGGASEVESQANRIAEATSSRVIRTYSAIA
                     AATGKNIVQLKADGKSAEELLTAVRQIPGVLGATPNYIFKILRTPNDPEFPSSWGMES
                     INASGAWEISTGSEEVYVALLDTGIQYDHEDLAANMGRDKDGNFGRNLVSAANATNPM
                     DDNGHGTHLAGIIGAAGDNRLGAAGINWTVKLLAVKVLDGNGAASVGHIIAGLDYVLE
                     QKSKGLNIRVANMAFGGWTPPASDVAADPLAIACKSLSDAGILLVAAAGNEGQNIARA
                     GGGYKGNTIYPAAFAFPEMVTVAAITQNGALAKFSNFGSSVDIAAPGYEVKSTALGGA
                     YASRNGTSVASAHVAGAAALLAAASPELTAGQIKTRLLEYAAGHQTLSGKVASGGSLN
                     IGAAIKNERGSLKVLIEGPASARWTLNGNGSYPSGQVLTDLPAGKHTVSFSDANLWKK
                     PANIAVSITSDSQAEATGKYRQQNASIWVTIEGPEEARWSLNKKGEYTNDQTLGKAAG
                     KRTVSFSEVPGWDAPAPQSVTVPRNAPLAVSGVYSRQTGSVTVTFEGPAEARWSLDGQ
                     GSYTSGETVENVPVGNRTVSFSKVTNWDTPQDVVVLVSKGASVAAEGKYIHQVGSIKV
                     TLEGPKEASWNVNGRGLFASGETASNIPVGEHTIAFSAVQDWDHPAAVPVTVVRDETV
                     EISRAYSKHLGSVSVTIKGPSDARWSLDGIDGMFESGAVRADLPVGEYAVVFKDVENW
                     YAPEKMNVTVVKDQVAQREASYAEHRGSVIVEIDGPAEARWSLDGEGSYASGE"
BASE COUNT          748 a          796 c          904 g          663 t
ORIGIN      
        1 gcgggcgcag agaagacagg tttgttgtac gagtatccag aagagaacca tagcgtagct
       61 gaacaggcga tacacatccg cacctcccgt acaagaaacg cttcacgggt gcgataaaaa
      121 gaaaatttgc gtttctgata aaaagctttt attgtattgt ttttatatat atactttcct
      181 tgctctcctg tcaatatcag gaagagcccc ctgtaataag cgttcagctg acaccgagat
      241 tgttggttaa acagcgcgga ttttttacga ctcagccata tccgaagctt tttttgtctt
      301 ttctcattga aatattgaaa gcaagcactc tacaaaagca aaaagttggt acaattagaa
      361 gtcgagggca agagaagcct ctaaaaaact gtgtgctgaa aaccgcgaaa agcggatgtg
      421 cattctttta attttctaca gtcatttttt ctcggggaag gaggaggatt aaaagctgcg
      481 aggaaagagt ttgtttgaag ccttgggcgg gtgttttttt tcgggttcat cgcacataat
      541 cgcgggagga tgaacaatgc ccggaagtgt ttttcggcgt ccggtcttca tactgggcga
      601 ggtttttatt cgatctatat cacagcacga atggatggtg atgggaatga aaaaaacgaa
      661 acttgcgttg atcctgcttt tcctgttcct gtgcgccggc tccgcctttg gccagccgaa
      721 agcgccgagc gacgaatatg tccggggaga tgttcttgtt gttcttgaag cgcccggggg
      781 cgcttccgag gtagagagcc aagcgaaccg gatcgccgaa gcgacctctt cgcgggtgat
      841 tcgcacctat agcgcgattg ccgccgcaac agggaaaaat atcgtgcagc tcaaggcgga
      901 cggtaaatca gcggaagaac tgctcaccgc agttcgccaa attcccggag tgctgggcgc
      961 gacgccgaac tacatcttca aaatcctccg aacgccgaat gatccagagt ttccgtcctc
     1021 ctggggtatg gagtctataa acgcatccgg agcatgggaa atatccaccg gcagcgagga
     1081 ggtctatgta gctcttcttg atacgggaat acagtacgac catgaggatc tggcagccaa
     1141 catgggacgc gacaaagacg gcaacttcgg aagaaacctg gtgagcgcgg cgaacgcgac
     1201 aaatccgatg gacgataacg gccacggaac gcaccttgcg ggtattatcg gcgcagcggg
     1261 agacaacagg ctgggcgcag ccgggatcaa ctggactgtc aaactgctgg cggtcaaggt
     1321 ccttgacggg aacggcgcgg ctagtgtcgg tcacatcatc gccggactcg actacgtgct
     1381 ggagcagaaa agcaaaggac tcaacatccg ggttgcgaat atggccttcg gaggatggac
     1441 tcctccggca tccgacgtcg ctgccgatcc tctggcaata gcctgcaaat ccctctccga
     1501 cgcaggtatt ctcctggttg ccgcggcagg gaacgagggg cagaacatag caagagcggg
     1561 cggcggatac aaagggaata cgatctatcc cgcagccttt gcgttcccag agatggtgac
     1621 agtagctgcg attacacaga acggagctct tgctaaattt tctaactttg gttcttctgt
     1681 ggatattgcc gccccaggct acgaagtaaa gagcacggcc ttgggtggag catacgcttc
     1741 gcgcaacggt acgtcggttg cttccgcgca tgtcgcgggc gctgcggccc ttcttgcggc
     1801 ggcgtccccg gagctgacgg cggggcagat caaaacacgt cttcttgagt atgccgcggg
     1861 ccatcagacg ctctccggta aggtagcgtc cggcggttcc ctgaatatcg gcgccgcgat
     1921 aaagaatgag agaggttctt tgaaagtcct catcgaaggg cctgcatcgg ctcgctggac
     1981 cttgaacgga aatgggagct atccaagcgg ccaggttctg acagacctcc ccgccggaaa
     2041 gcatacagtc tccttctcgg acgcgaacct ctggaaaaag cctgcgaata ttgcagtatc
     2101 gataacgtcc gattctcaag ccgaagcgac aggaaaatac cgccagcaga acgcgtccat
     2161 ctgggtcacc atcgaaggac ccgaggaggc tcgctggagc ctgaacaaga agggcgagta
     2221 cacaaacgac cagactctgg ggaaggccgc cggaaagcga actgtttcct tctccgaggt
     2281 tcccggatgg gacgctcctg cgcctcagtc cgtaactgtt cccaggaatg cgccgctcgc
     2341 agtttccggc gtctactcgc gccagaccgg ttctgtcacg gtaacattcg agggaccggc
     2401 cgaagctcgc tggagtcttg acggccaggg atcctatacg agcggagaaa ctgtagaaaa
     2461 tgttcctgtg ggcaaccgta cggtgagctt ctccaaagtt acgaactggg atacgcctca
     2521 agacgtagtt gttcttgttt ctaagggcgc ttccgtcgca gcggaaggga agtatatcca
     2581 tcaggtcgga tcaataaaag tgactcttga gggtcctaag gaggccagtt ggaatgtgaa
     2641 cggcagaggg ctttttgcga gcggcgagac agcctcgaac attcctgtcg gtgaacacac
     2701 gatcgctttt tccgccgttc aggactggga tcatcccgct gccgtcccgg tgacggtggt
     2761 ccgtgacgaa acagtggaaa tctcaagggc gtattccaag cacctcggct cggtctccgt
     2821 cacgatcaag ggaccctccg atgctcgctg gagcctcgat gggatcgacg gaatgtttga
     2881 gagcggcgca gttcgcgcgg atctcccggt cggcgagtat gccgttgtct tcaaggacgt
     2941 tgaaaactgg tacgctccgg agaagatgaa cgttaccgtt gtcaaagacc aggttgctca
     3001 acgagaagcc tcctacgccg aacacagagg atccgtcatc gttgaaatcg acggtcccgc
     3061 cgaagctcgt tggagcttgg acggcgaggg gagctacgca agcggagaga g
//
LOCUS       DAOPKU010000360         3110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35280_length_3110_cov_5.535843, whole genome shotgun sequence.
ACCESSION   DAOPKU010000360 DAOPKU010000000
VERSION     DAOPKU010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35280_length_3110_cov_5.535843"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="PK849_10705"
     CDS             <1..1211
                     /locus_tag="PK849_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPE66636.1"
                     /translation="AYKAGLSAGWYNAAWGMAGILAGIFAAHYFRRMEVNTIPEMMER
                     MYGKSARMISVVAQLLIMMVITSLQYVAGGAVLTALMPDVFTFQTGMMVTAVLFVGIT
                     FIGGYWGAGLTNVVNIIVIYGGILAALWNSSEVFGGFESIVAALPEGGPWFDFVSGAG
                     FAMVMSWMVVMITQGFSVQAISQIAFAAKNEKAARWGFILGGVIILPAGFLCALFGII
                     AAAKFPGLERAALALPTIMTNISPMVGGIFLAALWAAGISTAVGLLLGSSTLVMQDVW
                     KRFFPSTVTEKNELLLSRLMVLLFSGVTYFLALTSVGILRTITTALAITASFTLLILA
                     NIFFPKYCKKAAGFWTILASVAVWLAWTLYPQIRVVPHVVYLVWPACLATFALAYFLG
                     KEPAESIIKR"
     gene            complement(1270..2448)
                     /locus_tag="PK849_10710"
     CDS             complement(1270..2448)
                     /locus_tag="PK849_10710"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPE66637.1"
                     /translation="MLHDALAELPPGCAPACGACERPRSVEESLAGKKARAVRSLSRW
                     EDRIEDVVSPPEERRLGYRDRTALKALWTPEERWRFGMEGVRTDVRGPLRFARPRPFV
                     PLTDCPVHTERIRKMLHLPASALPPDEPEGSFPLSFLVVSGAQATFVFKAKTEPPSDG
                     WLFEPRDGSPSLADALGDIGIEGLWFHANPAAGVRLFAKRGWRLAWGAPRSADSRGLL
                     YGPASFSQLLPELHDASLDEARRFLVPGAGDAAADLYCGIGASLRLWRKCGADALGVE
                     GSGEAVECALHNAPGAAVLRGSCADRAPMISDFLRSVPPERRIAYVNPPRTGLEPQVT
                     ELLASHKLRGAAYLSCSPGTLARDLVILEKEYIVERLVPYDFFPRTAAVEVLALLRGR
                     "
     gene            2734..>3110
                     /locus_tag="PK849_10715"
     CDS             2734..>3110
                     /locus_tag="PK849_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE66638.1"
                     /translation="MKVMIVDDAAFMRMMIRKHLEGAGHEVVAEAFEYHSAMDEFKKL
                     LKTGTLPDAITMDITLPGRRSGIDITREIHRLAPEVRIVMTSAMGTQHMVIDAMKAGA
                     TDFVVKPFKPEQLLAALEGKPAE"
BASE COUNT          535 a          967 c         1038 g          570 t
ORIGIN      
        1 atgcgtacaa agcggggctc tcggcggggt ggtataacgc ggcgtgggga atggcgggta
       61 tcctcgcggg catcttcgcg gcgcactact tccgccgcat ggaagtgaat acgattcccg
      121 aaatgatgga gcggatgtac gggaaaagcg cccggatgat cagcgtggtg gcgcagctcc
      181 tcatcatgat ggtcatcacc tcgctgcagt acgtggcggg cggcgcggtg ctcacggccc
      241 tgatgcccga cgtcttcacc ttccagacag gaatgatggt cacggcggtc ctctttgtgg
      301 gcatcacctt catcggcggc tactggggcg ccgggctgac caatgtggtg aacatcatag
      361 tgatctacgg cggtatcctc gccgcgctgt ggaactcctc cgaggtcttc ggcggcttcg
      421 agtccatcgt cgccgcgctg cccgaggggg ggccgtggtt cgacttcgtc tccggcgctg
      481 ggttcgcgat ggtgatgtcg tggatggtgg tcatgatcac gcagggcttc tccgtgcagg
      541 ccatctcgca gatcgccttc gcggcgaaga acgagaaggc ggcgcggtgg gggttcatcc
      601 tgggcggcgt catcatcctt ccggccggct ttctctgcgc gctcttcggc atcatcgccg
      661 cggccaagtt ccccggcctt gaacgggcgg cgctcgcgct gccgaccatc atgacgaaca
      721 tctctcctat ggtgggcggc attttcctcg ccgcgctgtg ggccgcggga atctccactg
      781 cggtggggct gcttctgggg agctccacgc tcgtgatgca ggacgtctgg aaacgcttct
      841 tccccagcac ggtcacggag aagaacgagc tgttgctctc ccgcctgatg gtgctgctct
      901 tcagcggcgt cacctacttc ctcgcgctca cgtcggtggg catcctgcgg acgatcacga
      961 cggcgcttgc catcacggcc tccttcactc tattgatcct ggcgaacatc ttcttcccga
     1021 agtactgcaa gaaggccgcc ggattctgga ccatcctcgc ctccgtggcg gtttggctgg
     1081 cgtggaccct gtacccgcag atccgcgttg tccctcacgt ggtctacctc gtctggcccg
     1141 cctgcctggc gaccttcgcc ctcgcctact tcctgggcaa ggagcccgcg gagagcatca
     1201 tcaagcgctg aaaacgcgcg taacgcatac agcgaggggc cgctcgagga ttggaggtcc
     1261 ctcgcttttc tatctccccc gaagcagcgc gagcacttcg acggcggcgg tgcgcgggaa
     1321 gaagtcgtac ggaacgagcc gttccacgat gtactccttc tccagtatta ccagatcgcg
     1381 cgccagcgtg cccgggctgc acgagaggta ggccgctccc cggagcttgt gcgaggcgag
     1441 taactccgtt acctgaggtt ccagtccggt gcggggcggg ttgacgtacg cgatgcggcg
     1501 ttcgggcggg acggagcgca gaaagtccga gatcatcggg gcgcggtcgg cgcagcttcc
     1561 ccgcagcacc gccgctcccg gcgcgttgtg aagggcgcac tccaccgctt cgccgctccc
     1621 ctccacgccg agcgcgtccg ccccgcattt ccgccaaagg cgcaaacttg cgccgatgcc
     1681 gcagtagagg tcggctgcag cgtcacccgc ccccggaacg aggaagcggc gcgcttcgtc
     1741 gagcgacgcg tcgtgcagct ccggcagaag ctgcgagaaa gacgccggtc cgtagagcag
     1801 cccccgtgag tcggcggagc ggggcgcgcc ccatgcgagc cgccaccccc gcttggcgaa
     1861 gaggcgcact cccgccgcag gattggcgtg gaaccagagc ccttcgattc cgatgtcgcc
     1921 gagcgcgtcg gcaagggagg ggctgccgtc gcgcggctcg aagagccagc cgtccgaagg
     1981 cggctccgtc ttcgccttga agacgaacgt cgcctgcgcc ccggagacga cgagaaagct
     2041 gagtgggaag ctcccctccg gttcgtcggg cgggagggcg gacgccggca ggtgcagcat
     2101 tttccggatt cgttcggtat gcaccgggca gtcggtgagc gggacgaagg ggcggggacg
     2161 cgcgaagcgg agcgggccgc gaacgtccgt ccggacgccc tccatgccga aacgccacct
     2221 ttcttcaggc gtccaaagcg ccttcagggc ggttcggtca cggtacccca ggcgtcgttc
     2281 ctccgggggc gacacgacgt cctcgatgcg atcctcccag cgggagaggg agcggacggc
     2341 gcgggccttc ttcccggcga gcgactcctc gacggagcgc ggccgttcgc acgcgccgca
     2401 ggcgggggcg cacccgggag gaagttcggc aagagcgtca tggagcacgt atggtcacct
     2461 cgtcttcgtg tgttctcgct caagcatacc gcacgcgctg caagcgcgcc agactgcaat
     2521 cgaacagcac ggagaaagag agtgtgcgta ctttcgtcgc cgcaatctct cggaattgcc
     2581 ggaccctcgg cgtcctccgt atcgaagcgg cgctgtcaaa tcgattttcg ggggtgcgaa
     2641 tttccttcga agaggctata ctatgttaac tgggtattgt ttttttgctt cgatgaaagc
     2701 atgagaatga ctcgttcgac ggagggtacg ggaatgaaag tgatgatcgt ggacgacgcc
     2761 gcattcatgc gcatgatgat acgaaaacac ctggagggag cggggcacga agtcgtcgcg
     2821 gaggcgttcg aataccactc ggcgatggac gagttcaaaa aactgctgaa gaccggtacg
     2881 cttcccgatg cgatcacgat ggacattacg cttcccggca gacggtcggg gatagatatt
     2941 acgcgcgaaa ttcacaggtt ggctccggag gtcaggatcg tgatgacgtc cgcaatggga
     3001 acgcagcaca tggtgatcga cgcgatgaag gcgggagcga ccgatttcgt cgtcaagccg
     3061 ttcaagccgg aacagctcct cgctgcgctg gaggggaaac cggcggaata
//
LOCUS       DAOPKU010000361         3105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35376_length_3105_cov_4.482951, whole genome shotgun sequence.
ACCESSION   DAOPKU010000361 DAOPKU010000000
VERSION     DAOPKU010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35376_length_3105_cov_4.482951"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="PK849_10720"
     CDS             <1..1427
                     /locus_tag="PK849_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66639.1"
                     /translation="VLTGDSLRYNMLSREGILTGAVGSSPAGDGRVFLRGKQMEVAPV
                     DVARGKGWISSKGSKGVEEDELIGKLSDVSLTTCNERSPHYRLVSKSVVFVPGKRIIA
                     KNPKVYIGEHLLFSYPFDYVVPLDPKERRALLSSFFPLILSDSDKGQGVGIGGPYVWD
                     TGKALLNVAWWSDTGWEGKATVEQRLNDYWTVWATVEHSYEKESAEKLYRPSWGILHA
                     ANGWNASLRWTQREAISIQKKVGETYRGVLWREPEFSLLGPWGRAPLLNAYGRIGVSW
                     GEYEDVQPTVSMNVRRLGAEVQLYSEEQLESYKTFVKSEYKRFWYDDPDSLEQEIFDS
                     VFGIRYAWGTVDLSTAYVRRWVSGETPMLWDYYRDREDLYQQVVFPAGKNLSFRIRGG
                     YDLIDSKLAEMVYSVTLKGGECSRWELTYRNDRAEGDDWVGLSFFITAFPDTPVSFGR
                     RELYDPFALPDGLAEVLRRDEEEQ"
     gene            complement(1475..1936)
                     /gene="greA"
                     /locus_tag="PK849_10725"
     CDS             complement(1475..1936)
                     /gene="greA"
                     /locus_tag="PK849_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPE66640.1"
                     /translation="MTRKGYEKLMAELVELRSTGRAEISRQLEEARSFGDLSENAEYA
                     AAKEEQAKLETRISRLEFQLSNAKILDSSSIDASRVTLGTTVTIQDIINSQKFAYTIV
                     GSEESDPKASKISSSSPVGQALLNKTVGDEVHVKVPRGVRHLRVMDISIIS"
     gene            2122..>3105
                     /locus_tag="PK849_10730"
     CDS             2122..>3105
                     /locus_tag="PK849_10730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPE66641.1"
                     /translation="MARETRNTRKKRVGESTKKGGKLKYIPLGGLGQIGKNLHVFEYE
                     DSILVVDCGLMFPDDEMLGIDFVIPDISYLVENKEKIVGIVVTHGHEDHIGGLPFALK
                     KISAPLYGTKLTLGMVGNKLAEWAPELNPDYREIQAGDVVEMGPFKVRFIAVCHSIPD
                     GVGLAIGTPVGTVVHTGDFKLDPTPIDGRLTEYDAFSEEGKKGVLLLLSDSTNVERPG
                     FTESERVLSGTLDKIFRQYRSKRIVIASFASNLHRVQQVVDCAARFNRKVAFVGRSMV
                     KNVELARTLGYLHVDDKIIVDMEDISSVPSSSLVVMTTGSQGEPFSGLVLMS"
BASE COUNT          650 a          766 c          973 g          716 t
ORIGIN      
        1 gggtgctgac cggcgattcg ctgcgctaca atatgctatc ccgggaaggg atcctgaccg
       61 gagctgtcgg atcgtctcct gccggcgacg gtagggtgtt tctgcgcgga aaacagatgg
      121 aagttgcgcc ggtggatgtt gcccgcggta aaggctggat ttcctccaag ggaagcaagg
      181 gagttgaaga ggacgaactg atcggcaagc tgagcgacgt gtcgctgacg acgtgcaacg
      241 aacgctcgcc tcactatcgc ctggtctcga aaagcgttgt cttcgtccct gggaaacgga
      301 tcatcgcgaa gaatccgaag gtctatatcg gggagcacct tctcttttcc taccccttcg
      361 actacgtcgt gccccttgac cccaaggagc ggcgggcttt gctcagctct tttttcccgc
      421 ttatcctgag cgattcggat aaaggacagg gagtgggcat cggcggccct tatgtctggg
      481 atactggcaa agcgctgctg aacgtcgcct ggtggtccga taccggatgg gaggggaagg
      541 cgactgtgga gcagcggctg aacgactact ggaccgtatg ggcgacggtg gagcactcct
      601 atgaaaaaga gtctgcggag aagctctaca ggccgtcatg gggcatcctg cacgcagcga
      661 acggatggaa tgcctctctc cgttggacgc aacgcgaagc gatctccatc cagaaaaagg
      721 tcggagagac ctacaggggc gttctgtgga gagaacccga gttctctctg ctgggaccgt
      781 ggggcagggc gcctctgctc aacgcgtacg ggagaatagg ggtttcgtgg ggagagtacg
      841 aggacgttca gccgactgtc agcatgaatg tccgccgtct cggggcggag gtccagctct
      901 actcggagga gcagctcgaa tcctacaaga cgttcgttaa gagcgagtac aaacggttct
      961 ggtacgacga cccggattct cttgaacagg agatattcga ctccgtcttc ggaatccggt
     1021 atgcctgggg aaccgttgat ctttcaacgg cgtatgttcg aagatgggtt agcggcgaga
     1081 cgccgatgct gtgggattat taccgcgacc gtgaggacct gtaccagcag gttgttttcc
     1141 cggctggaaa gaacctctcg ttcaggattc gcggcggata cgacctcatt gattccaaat
     1201 tggcggagat ggtgtattcg gtaaccctga agggcgggga gtgttcccgc tgggagctta
     1261 cctacagaaa cgacagggcc gaaggagacg actgggtcgg cctctctttt ttcatcacgg
     1321 cgttcccgga tactccggtt tcgttcggac ggcgcgaact gtatgatccg ttcgctctgc
     1381 cggatgggct ggcggaggtt ctccgcaggg acgaagagga gcagtaaacg aatgctcctc
     1441 ctcgtccctg tgaagtgtga aagggctgaa cccgtcagga tatgatggaa atgtccatca
     1501 ccctgagatg ccgcaccccg cggggaacct tcacgtggac ttcatccccg acggtcttgt
     1561 tgagaagcgc ctgtcccacc gggcttgaag aggatatctt gcttgccttg gggtcggact
     1621 cttccgaacc gacgatcgtg taggcgaact tctgcgaatt gatgatgtcc tggatggtga
     1681 cggtggtccc gagggtaacc ctgctggcgt cgatgctgga ggagtcgagg atttttgcgt
     1741 tgctgagctg gaattcgagt cgagagatgc gcgtttccag cttggcctgt tcttctttcg
     1801 ccgcggcgta ctcggcgttt tcactgagat cgccgaatga tcgggcctct tcgagctgcc
     1861 gtgaaatctc cgctcttcct gtgctgcgga gttcgaccag ctcagccatg agcttctcgt
     1921 acccttttcg ggtcattgtg gatgggctgt cgttggtgtt ggtgttgttc aaacttgtaa
     1981 cctcccgaat atatcctcat tttgaggatt gggtgtattg cggttgatat ggaattctaa
     2041 caaagaaatt tctcttcggc aatacgtctt tgcatttttt gtagcgaaga tagccgaaat
     2101 tttgaaaggc aaggtggatt tatggcacgc gaaacgcgaa atacgagaaa aaagagagtc
     2161 ggggagtcaa cgaaaaaagg gggaaagctg aagtatatcc ctctgggagg gcttggtcag
     2221 ataggaaaga atcttcatgt gtttgaatat gaagattcga ttcttgtggt ggactgcgga
     2281 ttgatgttcc ccgacgacga gatgctcggt attgatttcg tcatcccgga tatttcctac
     2341 ctcgttgaga acaaggaaaa aatcgtcggt atcgtggtga cgcacgggca cgaggaccac
     2401 atcggagggc ttccgttcgc cctcaagaag atctcggctc ccctctacgg gacgaagctg
     2461 acgcttggta tggtgggcaa taagcttgcg gagtgggcgc ccgaactgaa tccggactac
     2521 cgggagattc aggccggcga cgtggtggag atggggccgt tcaaggtccg ttttatcgcg
     2581 gtctgtcact ccattcccga cggtgttggc ctggccatag gaacgcccgt cggcacagtg
     2641 gtgcatacgg gggatttcaa gcttgatcct acgccgatag acgggcgcct taccgagtac
     2701 gacgcgtttt cggaggaggg gaagaagggg gtgctgctcc ttctttcaga ttcgacaaat
     2761 gtggaaagac cgggattcac cgagtcggaa cgggtgctct ccggaacgct cgacaagatc
     2821 ttcagacagt accggtcgaa gcgtatcgtc atcgcctcgt tcgcgagcaa tctgcaccgt
     2881 gtgcagcaag tggtggactg cgcagcccgt tttaaccgaa aagtcgcttt cgtcggaaga
     2941 agcatggtga agaacgtcga gcttgcacgg accctcgggt atctgcacgt ggatgacaag
     3001 attatcgtcg atatggagga tatctcctcg gtcccttcaa gttcgctcgt tgtgatgact
     3061 acgggcagcc agggagagcc cttctccgga ctggtgctga tgagc
//
LOCUS       DAOPKU010000362         3104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35402_length_3104_cov_3.991473, whole genome shotgun sequence.
ACCESSION   DAOPKU010000362 DAOPKU010000000
VERSION     DAOPKU010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35402_length_3104_cov_3.991473"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PK849_10735"
     CDS             <1..468
                     /locus_tag="PK849_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE66642.1"
                     /translation="FEEIVDGAVRVLPELRAKVSYPVMEDRCYTGAPIRGSHYAPAWT
                     IPSDAPFLRVLSAALEEAGLCPDVSARPGFGTNGCHYAAERLLPTVVYGPSRRELVHT
                     VDEYIEIDALVDSCRGYFAMAERLLASRTLCGQGKKRVSVKEEKRRKVHGAAS"
     gene            452..1699
                     /locus_tag="PK849_10740"
     CDS             452..1699
                     /locus_tag="PK849_10740"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPE66643.1"
                     /translation="MAQPVELKCVLCGASYAPGEVEYTCPVCGLDGTLDVLYDYESAA
                     RTMTKGALAGRERSLWRYEEILPIVDKGSIPHLAVGWTPLYECPAVAEEYGVREFMVK
                     DDGRNPTGSLKDRASAVGVARAIDINQRTIACASTGNAASSLSGFAAVGGLRSFIFVP
                     EKAPVAKVTQLLVYGATVVLVKGDYADVFNLATAAINKYGWYNRNCAINPYLVEGKKT
                     CALEIAEQTGWNIPDRVFISVGDGCCIGGLYKGFSDLLRMGLISRMPKIVGVQAQGAK
                     PIYDAFKSKAERVAFGPADTVADSISVGAPRNWAKALRAVRESEGDMVAVSDEEILSA
                     IPELARKTGVFGEPAGAAAFAGFRKMAKEGLLGRGERIAVVVTGNGLKDIESARKSVG
                     APMLVEPTIEDFSGQLERSGFPC"
     gene            1693..2950
                     /gene="glyA"
                     /locus_tag="PK849_10745"
                     /pseudo
     CDS             1693..2950
                     /gene="glyA"
                     /locus_tag="PK849_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
BASE COUNT          635 a          865 c         1013 g          591 t
ORIGIN      
        1 ttcgaggaga tcgtggacgg ggctgttcgt gttcttccgg agctccgggc gaaggtctcc
       61 taccctgtga tggaggatcg ctgttatacg ggggccccca tcagggggag ccattacgcc
      121 cctgcgtgga cgatcccctc ggacgcgcct tttttgcgcg tgctttcggc cgcgctggag
      181 gaagcggggc tctgtccgga tgtgtcggcg cgtcctggct tcgggaccaa cggctgccat
      241 tacgcagccg agcggttgct tccgaccgtg gtgtacggac cctcccggcg cgagttagtg
      301 cacacagtag atgagtatat agaaatagac gcgcttgtcg attcgtgcag agggtacttt
      361 gcgatggcgg aacgcctctt ggcgtcccgg actctctgcg ggcaaggaaa gaaacgcgtt
      421 tccgtgaagg aagaaaaaag gaggaaagta catggcgcag ccagttgagt tgaaatgtgt
      481 tctgtgcgga gcttcgtatg ctccgggaga ggtggagtac acctgcccgg tctgcggact
      541 cgacggcacg ctcgacgttc tgtacgatta tgaatcggcg gctcgtacga tgacgaaggg
      601 ggcgcttgca ggacgcgagc ggtcgctgtg gcgttacgag gaaattctgc cgatcgtgga
      661 caaggggagc atcccccatc ttgcggttgg ttggactccg ctgtacgaat gtcctgcggt
      721 cgccgaggag tacggcgtga gggagttcat ggtgaaggac gacgggcgga atccgacagg
      781 gtcgctcaaa gaccgcgcca gcgcggtggg cgttgctcgc gcgatcgaca tcaaccagag
      841 gacgatcgcc tgcgcctcca ccggaaacgc cgcgagttca ctctcggggt tcgcggcggt
      901 gggggggctt cgcagcttca tcttcgttcc tgagaaggcg cctgtcgcca aggtgacgca
      961 gcttctggtc tacggcgcca cggttgtcct ggtgaagggc gactatgcgg acgtcttcaa
     1021 cctggcgacg gcggctatca acaagtacgg atggtacaac cggaactgcg cgatcaatcc
     1081 gtatctggtt gagggaaaga agacctgtgc gctcgaaata gcggagcaga ctggctggaa
     1141 tattccggac agggtgttca tctctgtggg cgacggctgc tgcatcggcg gattgtacaa
     1201 gggcttcagc gatctgcttc gcatgggact gatctcgcgg atgccgaaga tcgtcggcgt
     1261 tcaggcgcag ggcgcgaagc cgatctacga tgcgttcaaa agcaaggcgg agcgcgtggc
     1321 attcgggcct gcggatacag tagcggacag catatctgtc ggcgcgccgc ggaactgggc
     1381 caaggcgctt cgggcggtac gagaatccga gggcgacatg gtggcggtct ccgacgagga
     1441 gattctgtca gcgattcccg agcttgcccg gaagaccggc gtttttgggg aaccggcagg
     1501 agccgccgcg ttcgccgggt tcaggaagat ggccaaagaa gggcttctcg gacgcggcga
     1561 acggattgcc gtcgtcgtga ccgggaacgg tctgaaggat atagagagcg cgcggaagtc
     1621 ggtgggggcg ccgatgcttg tcgagccgac catcgaagac ttttccggac aacttgaaag
     1681 gagtgggttt ccatgttaga gaaggggcag gagtttcttc gggacgctga tcctgagatt
     1741 tttgcggcaa ttggcgagga gctggcccgc cagcggaacg gtatcgaact gatcgcgtcg
     1801 gagaacttcg tctcccgcgc ggtgctttcc gccatgggct cggtaatgac gaacaagtac
     1861 gccgaagggt acccgcacgc caggtactac ggcggctgcc acgtcgtcga caaggctgag
     1921 gacttggcgc gcgaccgggc aaaagagctc ttcgggtgcg atcacgtcaa cgtacagccg
     1981 cactccggat cgcaggcgaa tatggcggtc tacttctccg tgctgcagcc gggagatact
     2041 attcttgcca tgaacctcgc gcacggcggc catcttaccc acggatcgcc ggtgaacttc
     2101 tccggaaagc tgtataacat cattccgtac ggcgtcgaca agacgaccga gcggatcgac
     2161 ttcgccgagg tcgagcgcct cgccgtcgaa cacaaaccca agatgatcgt ctgcggcgct
     2221 tcggcctacc cccgcgagat cgacgcggag catttccgcg ctatcgcgga caaggtcggc
     2281 gcgctgcttt tcttcgacat cgcccatatc gccggattga tcgccgccgg atgccacaag
     2341 gatccggttc ccttctgcca cttccttacc tcgacgacgc acaagacgct ccgcggacct
     2401 cgcggcggca tgatcatgac caccgcggag tacgcgaagg ccatcgacaa gaacatcttc
     2461 cccggcatgc agggcggtcc tctgatgcac gtgatcgccg ccaaggccgt ggcgttccgc
     2521 gaagcgctca agccgtcctt caaggagtat cagaagaagg ttgtggcgaa cgcgaaagcg
     2581 ctcgcggaag agttcctctc cctcgattat catctggtct ccggcggtac ggataaccac
     2641 ctgattctgg tgaacctgat gaccaagggc gtcaccggaa aggccgcgga agccgcgctg
     2701 gacaaggctg gaatgacggt caacaagaat gctgtgcctt ttgataccca gccccttcat
     2761 caccagcggc atccgggtcg gcacgcccgc ggccacgact cgcggcttcg gcgtcgacga
     2821 catgaagcag atcgcccgct ggatcgaccg cgctgtgcgg aacgtcgaga atgaatccga
     2881 gcttgcgacg atcaaggccg aggttctgga gagctgcaac aagcaccccc tgtacccggg
     2941 gttggaataa ggagaattga tatgtcgaat tcgattgatc tgacgattca cgaagatgga
     3001 ctgaaacatg cggtagaggt agcgaaaaaa cgaaacatcg ttattcccac gttcaaacag
     3061 atgaaggatc ctctgaatca tacgccgaaa gcccttcagg agaa
//
LOCUS       DAOPKU010000363         3079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35805_length_3079_cov_4.832672, whole genome shotgun sequence.
ACCESSION   DAOPKU010000363 DAOPKU010000000
VERSION     DAOPKU010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35805_length_3079_cov_4.832672"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1505)
                     /locus_tag="PK849_10750"
     CDS             complement(159..1505)
                     /locus_tag="PK849_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPE66644.1"
                     /translation="MDLGHLIPRSFKGNLRTAVRGEGVYIYDEDGNAYMDGCCGALLS
                     SIGHGVKEIADAIHQQLLTMEFAHPSRWNNKVSVEAAEEITSLTQEGLDYVWLVSGGS
                     EAIESAMKIARQYFVERDGDGSGKYVMIARWNSYHGSTVGTMGLAGSMGRRRTFAPLF
                     MEGPKIQAHYCYRCPHNLKYPECNVACARALEGEILRIGPASVAAFFAEPIVGSTVGA
                     LHPPKEYWPIVREICSKYDVLLVADEIMTGMGRSGKPFCTQHWNVTPDIICSAKALAG
                     GYSPVGAMIVKKEIAETIRDGSGAFQHGHTYNGNPVTAAAVTATIRYMKKNNVFENAA
                     AMGARFDEKIPALNEIPIVGEVRGIGMMRGIEIVADRKTKAPFSPSVKAAALVTEECM
                     KRGLVVYPGTGQIGGVAGDQFLFAPPLVATKEEMDMMTDRLEAGLKAAAEALKGKG"
     gene            complement(1586..2431)
                     /locus_tag="PK849_10755"
     CDS             complement(1586..2431)
                     /locus_tag="PK849_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPE66645.1"
                     /translation="MLRERIASRLHEMTPGQKLVANYILEYAREAAFLTASQLGERVG
                     VSETTVIRLAHLLGFSGYQQLRSAMTNLLMDRLSTLERLKDYASTQEDDFFERALRKD
                     LETLTLTLSTICADDLKKLGAAIASAECVYLAGHRSSFSLAYYLSFYLSWILPNVKIV
                     SPDMPFEMLANAPKKSIVLGISFPRYSTWTVQVLETASELGLATACLTNDLASPLAVL
                     SEYVLAVPYKPISFIDSFAAPMSVLNCLILSVAQQLGDDVTKKLGILEKRWKEDDVYT
                     QQRGR"
     gene            complement(2704..2964)
                     /locus_tag="PK849_10760"
     CDS             complement(2704..2964)
                     /locus_tag="PK849_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66646.1"
                     /translation="MGAVQRVISVISDLAAVSEEQSASSSEMTRAVDQIARTTSSIAG
                     TVDTIRASSAETVQASMQIAEQATKLADRASVLQSMIAEFRV"
BASE COUNT          630 a          941 c          853 g          655 t
ORIGIN      
        1 ggtagtacgt accgcccacg ccgccgctgc cgatggtgat gaacgtatcg gccatacctg
       61 tggacgcgaa caccatgata ccaatgagcg caagtgccag aattcccttc ttcatgaaca
      121 atttcctcct tttttggtgc aagataaagg ggcgtttcct accccttccc tttgagcgcc
      181 tccgcggcag ccttcaaacc tgcttcgagg cgatctgtca tcatatccat ttcctctttc
      241 gtcgcgacca ggggaggagc gaagaggaac tgatcccccg ccacgccgcc gatctgtccc
      301 gttcccgggt acaccacaag accgcgcttc atgcactcct cggtgacgag ggcggccgcc
      361 ttcacggacg gcgagaacgg cgccttcgtc ttccggtccg cgacgatctc aataccgcgc
      421 atcatcccga tgccccgcac ttcgccgacg atgggaatct cgttcagcgc ggggattttc
      481 tcatcaaacc gcgcgcccat cgcggcggcg ttctcgaaga cgttgttctt tttcatgtag
      541 cggatggtcg ccgtcacagc ggcggcggtc accggatttc cgttgtaggt gtggccgtgc
      601 tggaaggcgc cgcttccgtc tcggatcgtt tcggcgatct ctttcttgac gatcatcgcc
      661 cccaccggcg agtatccacc ggcgagcgcc ttggcggaac agatgatatc cggcgtcacg
      721 ttccagtgct gcgtacagaa aggcttgccc gatcgtccca tgccggtcat gatctcgtcg
      781 gcgacaagca ggacgtcgta cttggagcag atctcgcgga cgatgggcca gtactccttc
      841 ggcggatgca gagcgccgac ggtggacccc acgatgggct cggcaaagaa ggccgccacc
      901 gaggccgggc cgatgcgcag gatctcgcct tcgagagcgc gggcgcaggc cacgttgcac
      961 tcagggtact tcaggttgtg cgggcagcgg tagcagtagt gcgcctgaat cttcggcccc
     1021 tccatgaaga gcggtgcgaa cgtgcgccgg cggcccatgc tgccggcgag ccccatcgtc
     1081 ccgacggtac ttccgtggta ggagttccag cgggcgatca tcacgtactt gccggaaccg
     1141 tcgccgtcgc gttcgacgaa atactgccgc gcaatcttca tcgccgactc gatggcctcg
     1201 ctgccgccgc tgacgagcca gacatagtca agaccttcct gcgtcaggga ggtaatctcc
     1261 tccgccgcct cgacggagac cttgttgttc cagcgcgacg ggtgggcgaa ctccatcgta
     1321 agaagctgct gatggatggc gtcggctatc tccttcacgc cgtgccctat gcttgagagc
     1381 aatgcgccgc agcacccgtc catgtaggcg tttccatcct cgtcgtagat gtagacgccc
     1441 tctccccgga cggccgttct gaggtttccc ttgaagcttc gcggtatcaa atgtccaaga
     1501 tccactaaga agcaactcct ttcatttttc ccgaactctt gtggtgcgtc ctgcgccgca
     1561 gacaggcaaa agcgttccga acgtctcaac ggcccctctg ctgcgtatac acatcgtcct
     1621 ccttccagcg cttttcaagt atcccgagct tcttcgtcac gtcgtcgccc aactgctggg
     1681 cgacgcttaa tataaggcag ttcaggacgc tcatcggcgc cgcgaaggag tctatgaatg
     1741 aaatcggttt atacggaacg gccaggacat actccgacag aaccgcgaga ggacttgcca
     1801 ggtcgttcgt cagacatgcg gtcgcaagcc ccaattcgga ggccgtttca agaacctgca
     1861 ccgtccacgt ggaataccgt ggaaagctga tcccgaggac aatacttttt ttcggcgcat
     1921 tcgccagcat ctcgaagggc atgtcggggc tgacaatttt cacgttcggc agaatccatg
     1981 agagataaaa agaaaggtag taggcaagag agaaagagct gcgatgtccg gcaaggtaca
     2041 cacattctgc ggacgctatc gctgcgccga gttttttcag atcgtcggcg caaatggttg
     2101 aaagggtgag cgtcagcgtc tcaaggtctt tgcggagcgc tcgctcgaag aaatcgtcct
     2161 cctgtgtgga cgcataatcc ttaaggcgtt caagcgtcga caggcggtcc atcagaagat
     2221 tggtcatcgc gctccgcaac tgctggtagc cggagaatcc cagaagatgc gccaggcgga
     2281 tcactgtcgt ctcgcttacc ccgacacgct ccccaagctg cgaagcggtc aggaaagccg
     2341 cctcgcgagc gtattcgaga atatagttcg ccacaagctt ctgccccggc gtcatctcat
     2401 gcagtcgtga tgcgatacgt tctctgagca tgcgccctcc ttgaaggaaa aaaatcctta
     2461 atcaatgcac aatgaagtat ttccttcacg gataataatc gaaaaactgc attttgtcaa
     2521 attaagtacc tccccagggt gtgcgaacag aaaaacgatg taggttttta agcccgaaaa
     2581 agcgcccttc atcaagagaa ggacgctttt tcggggctct tgtcgaacag ctctacccgc
     2641 cacataaatg cttcacagga gcatccgggg gggaactccg ccgcaggaag cctcgctaca
     2701 gtctcacact ctgaactccg caatcatact ttgcagcacg gaagccctgt cggccagttt
     2761 cgtcgcctgt tccgcgattt gcatcgacgc ctgcaccgtc tccgcggaag aggcgcgtat
     2821 cgtgtcgacc gttccggcta tcgaggacgt cgtccgcgct atctggtcca cagcgcgcgt
     2881 catttcggac gacgaggcgg actgctcctc ggaaaccgct gcaaggtcgg atatgacgct
     2941 tatcacgcgc tgcacagcgc ccattccttt tcgaagctcc tccatggatc gttgagagag
     3001 cgccgacgtc tccttgagga tcttgtcgaa aaggaatggg cgccgtgcag cgcgtgataa
     3061 gcgtcatatc cgaccttgc
//
LOCUS       DAOPKU010000364         3077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_35843_length_3077_cov_4.358041, whole genome shotgun sequence.
ACCESSION   DAOPKU010000364 DAOPKU010000000
VERSION     DAOPKU010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35843_length_3077_cov_4.358041"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PK849_10765"
     CDS             complement(<1..1319)
                     /locus_tag="PK849_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66647.1"
                     /translation="MNDEKAIVVRGLTFSYRGDEDVRAVKSLDFEVDRGEFVVIMGPS
                     GAGKSTLANSLNGLIPRFLRGKYEGSVLVNGLDAAKHSVSRMADEIGLVFQDFEAQLF
                     STNVKLEVAFGPENFAVPREEIAERIGRVLKTVNLEGVEGRSPATLSGGQKQRLAIGS
                     VLAMHPDIVCMDEPTTDLDPLGKMGVFAIAKELHEQGALTLLIIEHETEEALHADRLV
                     VMKDGEIAAQGKPGDILRDVPFTDGAGIMPLQIPKYFFELKLCEREELPLLPKDGAEF
                     FRRKGLVLEQSRYKWLIEGDRARERHYGKTIVKAEGVTHVYPDGNRAVDDVGLEIRER
                     EFVAILGHNGSGKTTLVKHFNGLLQPTSGKVTVCGKNTAEHSIYEIGKEIGYVFQNPD
                     HRIFAETVFEEVAFSPKIRGCPEEELAVRVREALEAVDLAGYEKQDP"
     gene            1587..2486
                     /locus_tag="PK849_10770"
     CDS             1587..2486
                     /locus_tag="PK849_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE66648.1"
                     /translation="MYLAGVLYCCAAAVCWALGPVFLKKGLALMSHSEMGAARTFGFV
                     GAALFFVMLEPGVAVGWNYPLPYLAVIFVSILIGNIVGDLAYFRSIEMIGVGRAVGTT
                     SCYPLFVTAISSVWLGEAVTLPLVLGTLVMIAGLVLLKSGGKQSAETRSVSGAGFFFA
                     IVAAVSWAGMMVLQKWLISVHSVHPASITLWRSFFLFSLSWLYWGTSSLRRGTNPMRI
                     FAMGPKAWGWAIAAGTFGLAAGGYFFTKGIQLIPVSIATPITASSPLLAAAMGVLFFH
                     ERMRPVQWAGILCILSGVLAVNS"
     gene            complement(2585..>3077)
                     /locus_tag="PK849_10775"
     CDS             complement(2585..>3077)
                     /locus_tag="PK849_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE66649.1"
                     /translation="ESNKAAEQIAALAAGITKDLDSVVASSERNAKDSRESSGLAENT
                     RLTIEKMMDALSHISMATQDLAAVSEEQAASSEEIAGAVQNIASRVSAAAASSDMVRG
                     QMAEVGTSAERVAQGSEELAGLADELRKLVGAFRFDGGAESKGLVPVKASGKNALVKS
                     GKK"
BASE COUNT          511 a         1059 c          837 g          670 t
ORIGIN      
        1 aagggatcct gcttctcgta tcctgcgagg tccaccgctt cgagcgcctc gcgcactctg
       61 acggcaagct cctcctccgg acaccccctg atcttcggac tgaacgcaac ctcttcgaag
      121 acggtctcgg cgaatatccg gtggtccgga ttctgaaaga cgtacccgat ctccttgccg
      181 atctcgtaga tcgagtgctc cgcagtattc ttcccgcaga ccgtcacctt gccggaggtg
      241 ggctgcaaca ggccgttgaa gtgcttcacc agcgtcgtct taccgctgcc gttgtgcccc
      301 agtatggcca cgaactcccg ctccctgatc tccaggccga cgtcgtccac cgccctgttg
      361 ccgtccgggt agacgtgcgt caccccctcg gccttcacga tcgtcttccc gtaatgccgc
      421 tcccgcgcgc ggtctccctc aatgagccac ttgtagcggg actgttccag aacaagcccc
      481 tttcgccgga agaactccgc cccatccttc gggagcagcg gcagttcttc ccgttcgcag
      541 agcttcagct cgaaaaagta cttcggaatc tgcaaaggca tgatgcccgc tccatcggta
      601 aacggcacgt cgcgcaggat gtcgcccggc ttcccctgcg ccgcgatctc cccgtccttc
      661 atcaccacga ggcggtcggc gtgcagggcc tcctccgtct cgtgctcgat gatgagcagc
      721 gtaagggccc cctgttcgtg cagctccttc gcgatggcga acacccccat cttccccagc
      781 ggatcgaggt cggtggtcgg ctcgtccata cagacgatat cggggtgcat cgcgaggacc
      841 gagccgatgg caaggcgctg cttctgcccg ccggagagcg tcgcggggct gcgcccctcc
      901 actccttcaa gattcacagt cttcagcacc ctgccgatgc gctccgcgat ttcctcccgg
      961 gggaccgcga agttctccgg gccgaacgcg acttcgagct ttacgttggt cgaaaagagc
     1021 tgcgcctcga agtcctgaaa cacaagacct atctcgtcgg ccatccggct gacggagtgt
     1081 ttcgccgcat ccagaccgtt tacgagcacg cttccctcgt acttcccgcg cagaaaacgg
     1141 gggatcagcc cgttcaggct gttcgccaga gtcgacttcc ccgcgccgct cggtcccatg
     1201 atcacgacga actcgcccct gtccacttcg aaatcgagcg acttcaccgc ccggacgtcc
     1261 tcgtcccctc tgtaggaaaa agtaagccct cgtaccacta tggctttttc atcattcatt
     1321 acgtaaatcc ccgccccttt cggtcggtag cgctcttgga atgcgcacat cggccgcgtt
     1381 tccttcgcag gagacacgaa tgcgccaccc cggattatac cagagggggc cggctctttc
     1441 cctgaaaaac cgaagggaac cgactccctt ttacacagag gacccggtct ccactctctt
     1501 tcgcacgaaa agaagtttct gtgctatcat tgcgtgcaat gtcacatcga tattccatgg
     1561 tccgccggac cgaggaggct actcaagtgt atcttgcagg tgttctgtat tgctgcgccg
     1621 cagctgtgtg ctgggctctc gggcctgttt ttctgaagaa agggctcgcg ttgatgagcc
     1681 attccgaaat gggagcggcg cggaccttcg gcttcgtcgg cgcggcgctg tttttcgtca
     1741 tgctggaacc tggcgtggca gtcgggtgga actatcccct tccctatctt gccgtcatct
     1801 tcgtcagcat cctcatcggc aacatcgtcg gcgacctcgc ctacttccgc tcgatcgaga
     1861 tgatcggcgt cggcagagct gtgggaacca cttcctgcta ccccctcttc gtcaccgcga
     1921 tctcttcggt ctggctcggc gaagccgtta cgcttcctct cgtacttgga acgctcgtga
     1981 tgatcgccgg gctcgttttg ctgaaatcgg gggggaaaca gtctgcggaa acgcgctcag
     2041 tctccggagc gggcttcttt ttcgccattg tcgcggcagt atcctgggcg gggatgatgg
     2101 tactccagaa gtggctcata tcggtccact ccgtccaccc tgcctccatc accctctggc
     2161 gctccttctt tctcttctct ctctcatggc tctattgggg aacaagttcc ctgcgccggg
     2221 gaacaaaccc gatgcggatc ttcgccatgg gcccgaaggc ctggggctgg gcaatcgccg
     2281 caggaacgtt cgggcttgcc gcaggggggt acttcttcac gaaggggatt cagctcatcc
     2341 cggtctccat cgccacgccg atcacggcaa gcagcccgct gctcgccgcg gctatggggg
     2401 ttctcttctt ccacgagcgg atgcgccccg tccagtgggc gggcatcctc tgcatcctga
     2461 gcggcgtcct cgcggtgaac tcgtagggaa cctcagagaa acaacgcaaa gacaaaagga
     2521 acggagcggg aggcgtacgc gcgttctccc gctccgttcc tttagagctt cccaaagatc
     2581 atcgttactt ctttccggac ttcacgagcg cattctttcc ggacgccttc accgggacca
     2641 gccccttcga ctcggcgcct ccgtcgaacc tgaacgcgcc cacaagcttc cgcagctcgt
     2701 cggcaaggcc tgcaagctcc tcggacccct gcgctacgcg ctccgccgac gtgccgacct
     2761 ccgccatctg tccgcgcacc atgtccgacg acgcggcggc ggcgctcacg cgggatgcga
     2821 tgttctgcac cgctccggcg atctcctcgc tcgacgcggc ctgctcctcc gagacggccg
     2881 ccaaatcctg cgtcgccatg gaaatgtgcg acagggcgtc catcatcttc tctatggtca
     2941 agcgcgtatt ttcggcgagc ccgctcgact ccctggagtc tttggcgttc cgttccgacg
     3001 acgccaccac gctgtccaaa tcttttgtga ttccggcggc aagcgccgca atctgctcgg
     3061 cggccttgtt gctctcc
//
LOCUS       DAOPKU010000365         3066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_36008_length_3066_cov_5.441049, whole genome shotgun sequence.
ACCESSION   DAOPKU010000365 DAOPKU010000000
VERSION     DAOPKU010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36008_length_3066_cov_5.441049"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..633)
                     /locus_tag="PK849_10780"
     CDS             complement(151..633)
                     /locus_tag="PK849_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66650.1"
                     /translation="METDAVTKLAQMHDGNAVGRFLSRASEFTDSCVVALAETPKQRG
                     NPDYPRMFAFSGDPGVAERMSFLYLDGLLPEGGFLFDAFGVVHVDDKPVLMYDAIHEA
                     VDRAVGPLEQLQGPSGILILASCGGVPWLPGASRGLVRTIGNPLAAYSALEFRLRAVT
                     "
     gene            complement(615..866)
                     /locus_tag="PK849_10785"
     CDS             complement(615..866)
                     /locus_tag="PK849_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66651.1"
                     /translation="MRQKQFNSLQHLTLRPEAWCGILGVRILDPDGWRQKKKDYAEFV
                     SLREFVELAQPSTMSFEDGATDFFDAENYLADGEWKQTQ"
     gene            complement(863..>3066)
                     /locus_tag="PK849_10790"
     CDS             complement(863..>3066)
                     /locus_tag="PK849_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66652.1"
                     /translation="TVFFKLNNLASMSVDQIDAPWELEVEGPPEGTKEEYKAWALNPI
                     TKHAFVSAVEGLSPVIRIGKDNPPVKMHGLIVDYDNRAVTADRLRELSDHPPCEWLPA
                     YGSVSFRKGAKLYWLFEKPIPVSPGVQVKKFLERLFKELRLNQWLAGYDAGATANIGQ
                     YYDIGKEFVAIGGAPIKHAVLAHWAWEATRDAVLWTDSKRYDIPIDEVAREMQERFPG
                     RWPGRFVEGARGVRFWDPAADNPTGAQVRRDGMTCYTGDRAFMSWADIFGSAFVSRFA
                     ATKTSELLDNTYYDGKRYYAKVPDEEGGHYVAWDKADFSQYLKVKGFDPARGKGKTCS
                     ELDEAEIAIKSQRLVDYAKKLVHFPRGLVQWRGKKYLNIGAPEPIKPAPQKDTKMSWS
                     DGEQYFPYIKSFMDVFFTDDKGEDDLNQRAFLFAWLKRFYEGGLTLTPTQGQVVIIAG
                     HPNKGKSFFCEGIVGGLMGGCEEGTSYLVDNVRWTEDVAESPVMYVGDSRAAEDHRAS
                     TAFSNQLKRIVANATLRSAQKFTKETDVPWYGRIMISCNLDAESLNILPNLEISNRDK
                     VLMFKTSSVQFPFPERRKSEELLKKELPFFARFLLDWDLPECVKSQMTRFGIEKFQHP
                     ELMEAASTQGSTGVLLDYLLSFLKADAAAAGHQRKCWIGTSAELYKVLAESDQQFGKE
                     LRTVRSFQTCLGQLKNRGVLRIDEEKPPRHGSNAAKVKKWVIWHPELEEES"
BASE COUNT          568 a          988 c          867 g          643 t
ORIGIN      
        1 cgagatgcgt catgtaccgg gtcttggacc gcatgtactc ctttgacttg tacgtcggca
       61 tgcgggagaa cagcgaccac cctccggcct ccggcaccgc gccaagggtg cccgtcacgc
      121 cgttgtccac tccgatgaag acacgtccgc tcacgtaacg gccctcagtc gaaactccag
      181 tgcggagtag gcagcgagcg ggttcccgat cgtcctcacc agcccgcgag acgcccccgg
      241 cagccagggc actcccccgc aagaggccag aataagaatt cccgagggtc cctgcagctg
      301 ctcaagcggg ccgactgcac ggtctaccgc ttcgtgtatc gcgtcgtaca tgagaacggg
      361 cttgtcgtcg acgtgcacca ccccgaacgc gtcgaacaga aagccaccct ccgggagcag
      421 cccgtccaag tataggaacg acatgcgctc ggccaccccc gggtccccag agaaagcaaa
      481 catacgtggg tagtccgggt taccccgctg cttaggggtc tccgccagcg caacgacgca
      541 ggagtcagtg aactccgatg cccgggacag gaagcgtccc accgcgttcc cgtcatgcat
      601 ttgcgcgagc ttggttactg cgtctgtttc cattcaccgt ccgccaggta gttttctgcg
      661 tcaaaaaagt ccgtagcccc gtcttcgaag gacatcgtgc tgggctgggc cagttctacg
      721 aactcacgaa gcgacacgaa ctcggcgtag tctttctttt tttgtcgcca tccatcgggg
      781 tccagaatcc ttacaccaag tatgccacac caggcttcgg ggcgcaacgt gaggtgctgc
      841 aacgaattga attgtttctg tctcatgact cttcctccag ctccgggtgc caaataaccc
      901 actttttcac tttcgccgcg tttgacccgt gccgaggcgg tttttcttcg tcgatgcgca
      961 gaaccccgcg gttcttcaac tgcccaaggc aggtctggaa gctgcggacg gtcctgagtt
     1021 ccttgccaaa ctgctggtcg gactccgcca agaccttgta cagctcggcg gacgtcccaa
     1081 tccagcactt ccgctgatgg ccggcagccg ccgcgtctgc tttcaggaac gacaacaggt
     1141 agtcgagcag gacgcccgtg gagccctgcg tcgatgcggc ctccatcagc tcggggtgct
     1201 gaaacttctc gatgccgaac cgggtcatct gcgacttgac gcattcaggt aggtcccagt
     1261 cgagcaagaa ccgggcgaag aacgggagct cctttttgag cagttcttcg gacttgcggc
     1321 gctctggaaa cgggaactgc accgaggacg tcttgaacat cagcaccttg tcccggttgc
     1381 tgatctccaa gtttggcagg atgttcagtg actcagcgtc caagttgcat gagatcatga
     1441 tccgaccata ccacggcaca tcggtctcct tggtaaactt ctgtgccgac cgaagtgtgg
     1501 cgttcgcgac gattcgtttt agctggttgg agaatgccgt ggatgcccga tggtcctcgg
     1561 cagccctcga gtcgccgacg tacattacgg ggctctccgc gacatcttct gtccagcgca
     1621 cgttatcgac gaggtagctc gtcccttcct cgcacccgcc catgagcccg cccacgatgc
     1681 cctcgcagaa gaagctcttg cccttgttcg ggtgacccgc gatgatcacg acctgcccct
     1741 gcgtaggcgt gagcgtcagc ccgccctcgt agaaccgctt gagccaggcg aacaggaacg
     1801 cccgctggtt caagtcgtct tcgcctttgt catcggtgaa aaacacgtcc atgaatgact
     1861 tgatgtacgg gaagtactgc tctccgtcgg accaggacat cttggtgtcc ttctgcggtg
     1921 ccggcttaat cggctccggc gctcctatgt tcaggtactt cttgccgcgc cactggacca
     1981 gccctcgcgg gaagtggacg agctttttcg cgtagtccac cagccgttgg gacttgatgg
     2041 cgatctcggc ctcgtccaac tcggagcagg tcttgccctt gccgcgagcg ggatcaaacc
     2101 ccttgacctt tagatactgc gagaaatcgg ctttgtccca agcgacatag tgcccgcctt
     2161 cctcgtccgg caccttggcg tagtaccgct tgccgtcata gtaggtgttg tcgagcagct
     2221 cggacgtctt ggttgcggcg aaccgcgaga cgaatgcgct gccgaagata tctgcccatg
     2281 acatgaacgc gcgatcgccg gtatagcaag tcattccgtc cctgcgaact tgcgcgcccg
     2341 tagggttgtc ggcggcgggg tcccagaaac gaacgccgcg cgctccttcc acgaatcggc
     2401 caggccaacg tcccgggaac cgctcctgca tttcacgggc tacttcgtca atcggtatgt
     2461 cgtaccgctt ggagtcggtc cacagaacgg cgtcgcgtgt ggcttcccat gcccagtggg
     2521 ccagaaccgc gtgcttgatc ggcgctccgc cgatggccac gaactccttc ccgatgtcat
     2581 agtattgccc gatatttgca gtagcgcccg cgtcgtatcc ggcgagccac tgatttaagc
     2641 gcaattcctt gaacagccgc tccaggaact ttttcacttg gacaccaggg gacacgggga
     2701 tcggtttttc gaacagccag tacagcttgg cccccttgcg gaacgagaca gacccgtacg
     2761 ctggcagcca ctcgcaaggc gggtgatccg acagctcccg caacctgtcc gccgtcaccg
     2821 cgcggttgtc gtagtccacg atcagcccgt gcatcttgac cggaggattg tccttgccga
     2881 ttcggatcac ggggctgagg ccctcaaccg ccgagacgaa cgcgtgcttg gtgatggggt
     2941 tcagcgccca cgccttgtac tcttccttcg taccctccgg cgggccttcc acctccagct
     3001 cccacggggc gtcgatctgg tcgaccgaca tggaagcaag gttgttcagt ttgaagaaca
     3061 cggtcc
//
LOCUS       DAOPKU010000366         3058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_36132_length_3058_cov_7.461205, whole genome shotgun sequence.
ACCESSION   DAOPKU010000366 DAOPKU010000000
VERSION     DAOPKU010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36132_length_3058_cov_7.461205"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1364
                     /locus_tag="PK849_10795"
     CDS             6..1364
                     /locus_tag="PK849_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE66653.1"
                     /translation="MERMYDLVIRGGSVVTPDGVLSTDVGIFGEKIAALGNALEGREM
                     IDAAGKLVFPGVIDPHVHFALPVGGSVSSDDFYSGSVAAACGGVTSVIDFTVGSSDSS
                     IPDDIRNRLEDAKNAVVDYSFHGEVVGWRPGRVDEMNEAIGLGVKSFKFFTAYGASGR
                     RTDTGPLLQAFQALADMDGVAVVHAEDDSMIETFSGLLSNEEWGRMESVAKVRPDVCE
                     ASAVNTVGWLAARTGVQCHIVHLSSALGLEEAFYARERGADLTVETCPQYLLLTSDVY
                     KGPEGHLYSAAPALRSSDDNAVLWNALASGEIDFAATDHCPFTRTQKEWKGAFDRLPG
                     GLPGVELLLPLLYSEGVLRGRLPLCELARITSERAARRYGLYPRKGSLLPGSDADIVL
                     FDPEEVWHVQAGALRMNVDFSPYENLELIGKTWQTISRGEIIYSEGRFLGKKGRGVFL
                     HR"
     gene            1419..>3058
                     /locus_tag="PK849_10800"
     CDS             1419..>3058
                     /locus_tag="PK849_10800"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPE66654.1"
                     /translation="MHVPSDIEIAQGSELRPIVEIAEKLGIGESELEQYGKYKAKISP
                     EVWDRVKGNADGKLILVTAITPTPAGEGKTTTTVGLAQALAKRGKKTSLAIREPSLGP
                     SFGVKGGAAGGGYSQVIPMEDINLHFTGDLHAITTAHNLISAMVDNHIQQGNELNLDP
                     RRIVWRRVMDLNERALRSVIVGLGGKANGVPRESGFDITVASEVMAILCLSTDLMDLK
                     TRIAKIVVGYTYDGKAVTVGEIGAAGSAAVLLKDAVKPNLVQTLEGVPAFIHGGPFAN
                     IAHGCNSIQATRLGLKLSDYFVTEAGFGADLGAEKFLDIKCRLGGLTPSAVVIVATVR
                     ALKMHGGRKKSELGEKDLVALEKGMTNLEKHIENIASFGLPAVVAINRFPADSEDELA
                     LIEKKCNALGASVALSEVWEKGGDGGLELADKVMEACGKKSEFVYQYEVGMSPKEKIE
                     RIAKNIYGASGVTYTDQAEKDLAQIHELGKDELLVCMAKTQYSLSDDPALLGRPSGFT
                     VTVREVRLSAGAGFLVAVTGSIMTMPGLPKKPAALSID"
BASE COUNT          651 a          824 c         1034 g          549 t
ORIGIN      
        1 ggaggatcga acgcatgtac gatcttgtga tacgaggagg ctccgtggtg acccccgacg
       61 gagtgctctc taccgatgtc gggatcttcg gggagaagat cgccgcactt ggtaacgcgc
      121 tcgaagggcg ggagatgatc gacgcggcgg ggaagcttgt ttttcccgga gttatcgatc
      181 cgcacgtaca cttcgccctg cctgtcggag gctcggtttc aagcgacgac ttctattcag
      241 ggagcgtcgc cgctgcgtgc ggaggggtaa catcggttat cgacttcacc gtcggctcct
      301 ctgacagcag tatccccgac gacatccgga accgtcttga ggacgcaaaa aacgccgttg
      361 tggactactc gttccacgga gaggtcgtgg gatggcgtcc cggaagagtc gatgagatga
      421 acgaagcgat cggtctcggc gtaaagagct ttaaattttt tacggcctac ggcgcttccg
      481 gacgacgtac ggatacgggg ccgctgctcc aggcttttca ggctttggcc gacatggatg
      541 gcgtcgccgt cgtccatgcg gaggacgaca gcatgattga gacgttctcc ggccttctct
      601 cgaacgagga gtgggggcgt atggagagcg tggccaaagt acgccccgac gtctgcgagg
      661 cgtccgccgt gaatactgtc ggctggcttg cggccagaac cggggtgcag tgccatatag
      721 tccacctcag ctctgcactt gggcttgagg aggcgttcta tgccagagaa agaggcgccg
      781 atctgactgt cgagacctgt cctcaatacc tgttgcttac ttccgacgtc tacaaaggtc
      841 ccgagggcca tctgtactcg gcggctcccg cactgaggag ttcagacgac aatgctgttc
      901 tttggaacgc tcttgcctcg ggcgagattg attttgccgc cacggaccac tgtcccttca
      961 cacggacgca gaaagagtgg aagggggcgt tcgaccggct tccgggagga ctgcccggcg
     1021 tggaactgct tcttccgctg ttgtactccg aaggtgtgct tcgaggcagg ctgccgttgt
     1081 gcgaacttgc gcggattacc tcggagcgcg cagcgaggcg ttacggtctg tacccgcgga
     1141 aggggagcct gctgcccggt tcggacgccg acatcgtgct ctttgacccg gaagaggtct
     1201 ggcacgtaca ggccggggcg cttcgaatga atgttgattt ctctccgtat gaaaaccttg
     1261 aactgatcgg aaagacgtgg caaactattt cacgggggga aattatctac tcggaagggc
     1321 gtttcctggg aaaaaaagga cgaggagtgt ttttgcaccg ttaatcgtta ccccgcgcca
     1381 acatgaaggg gtattttatc aaggaggact gagaagtaat gcatgtgccg agcgacatag
     1441 agatagcgca gggatcggaa ctgcggccga tagtggagat agcggagaag ctgggaatcg
     1501 gagagtcgga gcttgagcag tacgggaagt acaaggcgaa aatatcgccg gaagtatggg
     1561 atcgggtgaa ggggaacgcg gacgggaagc tgatattggt gacggcgatc acgccgacgc
     1621 ccgcgggcga agggaagacc accacgacgg tcggacttgc gcaggcgctg gcgaagcgcg
     1681 ggaagaagac gagcctcgcc atccgggagc cgtccctggg ccccagcttc ggcgtcaagg
     1741 gaggggccgc cggaggcggt tacagccagg tcatccccat ggaggacatc aacctccact
     1801 tcacgggcga ccttcacgcg atcaccacgg cgcacaacct gatctcggcg atggtggaca
     1861 accacatcca gcaggggaac gagctgaacc tcgacccgcg gcggatcgta tggcgccgcg
     1921 tcatggacct gaacgaacgg gcgcttcgga gcgtcatcgt cggactcgga ggcaaggcga
     1981 acggcgtgcc gcgcgaaagc ggattcgaca tcaccgtggc ctctgaggtc atggcgatcc
     2041 tgtgcctgtc gaccgacctc atggacctga agacgcggat cgcgaagatc gtcgtcggct
     2101 acacgtacga cggcaaggcc gtcaccgtgg gagagatcgg agcggcggga tccgcggcgg
     2161 tgctgctgaa ggacgccgtg aaaccgaacc tcgtacagac gctcgaagga gtgcccgcgt
     2221 tcatccacgg aggccccttc gcgaacatag cgcacggatg caactccatc caggcaacgc
     2281 ggctgggctt gaagctctcg gactacttcg tcacggaagc cggattcgga gcggacctcg
     2341 gagcggagaa gttcctggac atcaagtgcc gtctcggcgg actcacgccc tcggcggtcg
     2401 tcatcgtcgc caccgtgcgc gcgctgaaga tgcacggagg gaggaagaag agcgaactcg
     2461 gcgagaagga ccttgtcgcg ctggagaagg gaatgaccaa cctcgagaag cacatagaga
     2521 acatcgcgtc gttcggcctt cccgcagtcg tcgccatcaa ccgctttccc gccgacagcg
     2581 aagacgagct agcgctcatc gagaagaaat gcaacgcgct cggcgcgtcg gtagccctgt
     2641 cggaagtgtg ggagaagggc ggagacggcg gccttgaact cgcggacaaa gtgatggaag
     2701 cgtgcgggaa gaagtcggag ttcgtctacc agtacgaagt aggaatgagc ccgaaggaga
     2761 agatagagcg gatcgcgaag aatatctacg gagcgtcggg agtgacgtac acggaccagg
     2821 cggagaagga cctcgcgcag attcacgagc ttgggaagga cgagttgctg gtgtgcatgg
     2881 cgaagacgca gtactcgctc tctgacgacc ctgcgctgct ggggagacct tcgggattca
     2941 cggtgacggt gcgcgaagtg agactgtctg cgggggccgg attcctggtg gcggtgacgg
     3001 gatcgatcat gacgatgccg ggattgccga agaagcctgc ggcgctgtcg atagacat
//
LOCUS       DAOPKU010000367         3044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_36389_length_3044_cov_4.663767, whole genome shotgun sequence.
ACCESSION   DAOPKU010000367 DAOPKU010000000
VERSION     DAOPKU010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36389_length_3044_cov_4.663767"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="PK849_10805"
     CDS             complement(<1..1904)
                     /locus_tag="PK849_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE66655.1"
                     /translation="MSTYISQEQKLKIQDEFKEYLKKEHPGWVERTVYTNVSDSFYVF
                     NNDIGMNPWSCFVSDALMEEAREVLTEYFRVHDKPGNPETRANGYIKSMQLLKLFLDR
                     NYPGLASEQSAHTHAPSCWIFQCNPKYYDLLRALREKETLRWSINQYAKRIQAGDKVY
                     LWMSGSDGGIVASGKVDSAPEKRAPDLNDAYWMDGYRPTEEYLCVDISLERKFADSVI
                     SRSVLSADERTKKLGILLFPQGTNFHVAREQEAVIESILEGAYVRVPATSDDATAEGK
                     RYWMYAPGKGAEKWDEFHKHGIMAVGWDELGDLSRYSSKQNIKEKMKEVYGEDKNYMN
                     DGHALWQFANDVKPGDVVFVKKGKNMIVGRGEVTSEYFFDESRAEYQHIHEIDWTHSG
                     EWSAEKDLSVKTLTDFTPYTEACRLLEEKFEGDVVIEDDPPKTFARYTQDDFLNDVFM
                     SEDRYVTLKNLLLKKKNIILQGAPGVGKTYAAERLAYSIMEEKDTNRVMTIQFHQSYS
                     YEDFIMGYRPTENGFSMGKGPFYSFCKKAEPDDREHFFIIDEINRGNLSKIFGELLML
                     IENDKRGKSIRLLYSDEQFQVPKNVHIIGMMNTADRSLAMIDYALRRRFAFFEFEPAF
                     ASTGFKRYQE"
     gene            complement(2135..2554)
                     /locus_tag="PK849_10810"
     CDS             complement(2135..2554)
                     /locus_tag="PK849_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPE66656.1"
                     /translation="MSVRFVVDTSVVMSWCFEDEGNGYAETVLESLESAEAIAPAIWP
                     LEVGNVLLVAERKKRLGQAASVRFLSLLGALPITVEQEPSERMLKEILSLARTLGLST
                     YDASYLDLAMRLDLPLATLDAALANAAKKSGVQIYRP"
     gene            complement(2554..2793)
                     /locus_tag="PK849_10815"
     CDS             complement(2554..2793)
                     /locus_tag="PK849_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPE66657.1"
                     /translation="MEKIGVYEAKTHLTQLMDRVSKGEKVTITKHGVPVAILQPADFS
                     KTEPVREIIEQLKQFRSGRRLDGLSIRDMIEEGRH"
BASE COUNT          617 a          840 c          807 g          780 t
ORIGIN      
        1 aattcctgat acctcttgaa tccggtcgat gcgaaggcgg gctcgaattc gaaaaatgcg
       61 aaacgccggc gcagcgcata gtcgatcatg gccaagctgc gatccgccgt gttcatcatg
      121 ccgatgatat gtacattctt gggaacctga aactgctcgt ccgaatagag gaggcggatg
      181 gattttccgc gcttgtcgtt ctcgatgagc atcaggagtt cgccgaatat cttgctgagg
      241 ttcccgcggt tgatctcgtc gatgatgaag aagtgctcgc ggtcgtccgg ttccgccttt
      301 ttgcagaagc tgtagaacgg ccccttcccc atcgaaaaac cgttctccgt cgggcgatac
      361 cccatgatga aatcctcgta gctgtagctc tggtggaact gaatcgtcat gacccggttc
      421 gtgtccttct cctccatgat cgagtaggca aggcgctccg ccgcgtacgt ctttcccacg
      481 cccggcgctc cctgaaggat gatgttcttc ttcttgagca ggaggttttt cagcgtgacg
      541 taccggtctt cgctcatgaa gacatcgttc aggaaatcgt cctgtgtgta gcgtgcgaag
      601 gttttcggcg gatcgtcctc gataactaca tccccttcga acttctcttc caacagccga
      661 cacgcttcgg tgtagggcgt gaagtcggtg agcgttttga cggacaggtc cttttcggcg
      721 ctccattcac ccgaatgcgt ccagtcgatc tcgtgaatat gctggtattc ggcgcgggat
      781 tcatcgaaga aatactccga agtcacctcc ccgcgcccga cgatcatgtt cttccccttt
      841 ttaacgaaga cgacgtctcc cggcttcaca tcgttggcga actgccagag cgcatggccg
      901 tcgttcatgt agttcttatc ctctccgtaa acctctttca ttttctcctt gatgttttgt
      961 ttggaggagt accgagaaag atcgcccagt tcgtcccaac cgaccgccat gatcccgtgt
     1021 ttatggaatt catcccactt ctccgctcct ttgcctggag cgtacatcca gtaccgcttc
     1081 ccttccgcgg tggcgtcgtc gctcgttgcc ggtacacgaa cataggcgcc ttccagtatg
     1141 ctctcgatga cggcctcctg ctcccgagcg acgtgaaagt tcgttccctg cgggaagagc
     1201 aggatcccca gttttttcgt tcgctcgtcg gcggaaagaa cgcttctgga aatgacagag
     1261 tcggcgaact tgcgttccaa agaaatgtcg acacacagat attcctcggt cgggcgataa
     1321 ccgtccatcc agtaagcgtc gttcaaatcg ggcgcccttt tctccggagc tgaatctacc
     1381 ttccccgagg cgacgatgcc tccgtcggaa ccggacatcc agagatacac cttgtctccg
     1441 gcctgaatcc gtttcgcgta ctgattgatg ctccagcgga gcgtctcctt ttcccgcagg
     1501 gcccgaagaa ggtcgtagta tttggggttg cactggaata tccagcagga gggggcgtgc
     1561 gtgtgcgctg actgttcgga ggcgaggccg gggtagttgc gatcaaggaa cagcttgagg
     1621 agttgcatgg atttgatata gccgttcgcg cgtgtttcgg gatttcccgg cttgtcatgt
     1681 actcgaaaat actccgtgag cacttcccgg gcctcttcca tcaaggcgtc gctgacgaag
     1741 caggaccaag ggttcatccc gatatcgtta ttgaagacat agaaggagtc gctgacgttc
     1801 gtatacactg ttctttcaac ccatcccgga tgttcttttt tcagatattc tttgaactcg
     1861 tcttggattt tcagcttttg ctcctgtgag atataggtgc tcatacgtgt gcctccctca
     1921 ttcagggcga agcgctcccg caaacgccgg cgatgccctg atttttccgt atccggcatt
     1981 ggtatcccga cctgtttttc atcatgtgag gagctgtatt gagacgctcg cagaatgcaa
     2041 cggagagcct cgttttccac agtccaatat atacgttgaa gtcttattgt caagtatttc
     2101 gtccggcctc cgcttccggg agcactcttc gccgttacgg cctgtagatc tgtactccgc
     2161 ttttcttcgc cgcattcgcg agggcagcgt ccagcgtggc gagcggaaga tcgagccgca
     2221 tggcaagatc caggtaggat gcgtcgtagg tggacagtcc gagggtacgg gcgagcgaaa
     2281 ggatttcttt caacatgcgt tcggagggtt cctgttccac agtgatcggc agcgcgccca
     2341 ggagggagag aaagcgaacg gacgcagcct gaccgagtcg ttttttcctt tcagccacaa
     2401 gcagcacatt ccctacttca agaggccaga tcgcgggtgc gatggcctcc gccgattcca
     2461 gactttcgag aacggtttcc gcatacccgt tcccttcgtc ctcgaagcac catgacatga
     2521 cgacggaggt atccaccacg aagcgcacgg acatcagtgc cttccctcct cgatcatgtc
     2581 gcggatggag agtccgtcga gccggcggcc gcttcggaat tgcttcagtt gttcgatgat
     2641 ctcccgtacg ggttccgtct tcgaaaagtc agcgggctgc aaaatcgcca cgggcacgcc
     2701 gtgcttcgtg attgtaactt tttcaccctt ggaaacgcga tccataagtt gcgtgagatg
     2761 tgttttcgcc tcgtagactc cgattttttc catattctcc cctccgttta aaccagttta
     2821 agaccagtct atatcatacc gtcggaaaat tcaagagagt gagtctcact gtagagacta
     2881 tttctggatt tcctcgaggt tttgatcatc ggaaaaagaa gactgccagc gacgccagta
     2941 tcgggtatca cccttgagtt gtaacgcaaa aagcaataca atgtgtcctg ggggtgagcg
     3001 gtatggtgaa tgcgaccgta aatatcagat tggaagccga ggat
//
LOCUS       DAOPKU010000368         3039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_36473_length_3039_cov_4.149129, whole genome shotgun sequence.
ACCESSION   DAOPKU010000368 DAOPKU010000000
VERSION     DAOPKU010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36473_length_3039_cov_4.149129"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="PK849_10820"
     CDS             complement(<1..155)
                     /locus_tag="PK849_10820"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HPE66658.1"
                     /translation="MSNAKNRLAKEKSPYLLQHAENPVDWYPWGEEAFAKARTEDKPL
                     FVSIGYAT"
     gene            complement(148..795)
                     /locus_tag="PK849_10825"
     CDS             complement(148..795)
                     /locus_tag="PK849_10825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPE66659.1"
                     /translation="MFSCVIFDFDMTLVDSSYAIRDTMNMVAEREGLPKVTREDVLSV
                     IGLPIRDSWLKVWGKFDERWLADFREMFVQQEFAGIFPFSGTKQVLETLAARGVSLGI
                     ASNRQRPGGPLKAVGLESYFSSVVGMDDVEKGKPAPDMLLKGMADLGGTPATTLYVGD
                     TADDMTAAKDAGIRSVGLTTGNFTPEALHAAGAWKVYDAVEEILPLFGTEGGRSE"
     gene            complement(902..1462)
                     /locus_tag="PK849_10830"
     CDS             complement(902..1462)
                     /locus_tag="PK849_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE66660.1"
                     /translation="MSRFDIYMSGVGGQGIGLLSEAIIRAADYAGLEVCGVDTHGLAQ
                     RGGMVESFVRIGTLTTPLVRKGSAKLVLALERTEALRAVELFAAPGGTLLYYDTCWQP
                     LSVRLGTEKETSAEDVAAACARLDVRVIPVRTELPDVRMQNVALLAVAAKEKLIPGVG
                     VEHYEKALADLLPERALQGNLKVLKG"
     gene            complement(1459..>3039)
                     /locus_tag="PK849_10835"
     CDS             complement(1459..>3039)
                     /locus_tag="PK849_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE66661.1"
                     /translation="QLSLLELGGGLVAILGDDPGANSSQNEQDNRHIARLAYIPVLEP
                     ASPAEAYTFYLEAMKLSRKRKMPVILRMTTHVCHGKEKVSFAGWTPRPIGSVPAFDSS
                     AGPSIPLTTRALDMKRRAIARLAEVAQETDGSPLHELREGNDPSKGVITAGLPFRSLV
                     DVLEDAAEKPDILKLGVVHPLPKGLVADFLRSHASVKVLEELDDVLEREIKALAFDLG
                     IRTVIVGKTDPEDWIGEYTPDKVAEVLAKTWPSLVPRKSAERAATLAPRPPQMCPGCG
                     HRSAFHAIKQALAKEDITVADIGCHTLGFLPPYKMGEVLLSMGHSTGTAAGLSLFNTT
                     RKVVAFLGDSTFFHAGLPGIVNAVFNNHNLTLILMENGTTAMTGHQDHAGSGGNANGP
                     ADAIPVRAVLEGLGVKSIREADTYSQAKLTQMVKEAVAEEGFSVVIARHPCMLKYTRE
                     QQRTPGYVRKSVNIDQEKCDKLHVCVESFGCPSFQRDEEGRVVVSEELCIGDGSCIQT
                     CPAKAIGVKKVQEGGGKA"
BASE COUNT          481 a          968 c         1028 g          562 t
ORIGIN      
        1 gtggcgtacc cgatggagac gaagagcggc ttgtcctccg tcctggcttt cgcgaaggct
       61 tcctctcccc aggggtacca atcgacgggg ttctccgcgt gctggagcag gtagggggat
      121 ttttcctttg cgaggcggtt ttttgcgtta ctcactgcgg ccgccctccg ttccgaagag
      181 ggggagaatc tcttccacgg cgtcgtagac cttccacgcg cccgcggcgt gaagggcttc
      241 gggcgtgaag ttgccggtgg tcagtccgac cgaacggatt ccggcgtctt tggcggcggt
      301 catgtcgtcg gcggtgtcgc cgacgtagag agtggtcgcg ggcgtgccgc cgaggtcggc
      361 catccccttg aggagcatat cgggggcggg cttgcccttc tccacgtcgt ccattcccac
      421 cacggagctg aagtagctct ccagcccgac ggccttcaag gggcctccgg gtcgctgacg
      481 gttggaggcg ataccgaggg agacgccgcg agccgcgagc gtttcgagca cctgcttcgt
      541 cccggagaag ggaaagattc ccgcgaactc ctgctgaacg aacatctcgc ggaaatcggc
      601 gagccagcgc tcgtcgaatt tgccccaaac cttcagccag gaatcgcgta tcggcagacc
      661 gatgacggag agcacgtcct cccgcgtgac tttggggagt ccctcccgct cggcgaccat
      721 gttcatcgtg tcccgtatgg cgtagctgct gtccacaagg gtcatgtcga agtcgaagat
      781 gacgcatgaa aacatcgaat ccactccttt tctcgaatcg tccagggaaa tggtactcta
      841 tgggtcggag aaaagcaaag gccgcccccg caggaacggc ctttgcgtac agccggcttc
      901 gctacccttt gagcaccttc aggttgccct ggagcgcccg ctcgggaagc aggtcggcaa
      961 gcgccttctc gtagtgttcg acgccgacgc cgggaatcag cttctctttg gccgcgacgg
     1021 cgagcagcgc gacgttctgc atccgcacgt ccggcagctc cgtccgtacg gggatcacgc
     1081 gcacgtcgag acgggcgcag gccgcggcca cgtcctccgc ggaggtctcc ttctccgtgc
     1141 cgaggcggac ggagagcggc tgccagcatg tgtcgtagta gagcagcgtt cctccgggcg
     1201 cggcgaagag ctccacggcc cgcagcgcct cggtgcgttc gagggcgagc acgagcttgg
     1261 cgctcccctt gcggaccagc ggcgtcgtca gcgtcccgat gcggacgaac gactcgacca
     1321 tgccgccccg ctgggcgagg ccgtgcgtgt ccacgccgca gacctcgagc cccgcgtagt
     1381 ccgcggccct gatgatcgcc tcgctcagca gaccgatccc ctggccgccg acgcccgaca
     1441 tgtagatatc gaagcggctc atgccttccc gcctccttcc tgaactttct tcacgccgat
     1501 cgccttcgcc ggacaggtct ggatgcagga gccgtcgccg atgcacagct cttcgctcac
     1561 gacgacgcgt ccctcttcat cgcgctggaa cgaggggcag ccgaaggact cgacgcagac
     1621 gtgcagtttg tcgcacttct cctggtcgat attcaccgat ttacggacgt atcccggtgt
     1681 tcgctgctgc tcgcgcgtgt acttcagcat gcacgggtgg cgggcgatga cgacagagaa
     1741 cccctcctcc gcgacggcct ccttcaccat ttgtgtcagc ttcgcctggc tgtaggtgtc
     1801 cgcctcgcgg atgctcttca cccccagccc ttcgagcact gcacggacgg ggatggcgtc
     1861 ggcggggccg ttggcgttgc cgcccgagcc cgcgtggtcc tggtgccccg tcatggcggt
     1921 ggtgccgttc tccatcagaa tgagcgtgag gttgtggttg ttgaagaccg cgttgacgat
     1981 gccggggagt ccggcgtgga agaaggtgga gtcgccgaga aaggcgacaa ccttgcgcgt
     2041 ggtgttgaag agcgagagcc ccgccgccgt gccggtggag tgccccatag aaagaagcac
     2101 ctcgcccatc ttgtagggcg ggaggaagcc gagggtgtgg cagccgatgt ccgcgacggt
     2161 gatgtcctcc ttcgcgaggg cctgcttgat ggcgtggaag gccgagcggt gaccgcatcc
     2221 ggggcacatc tgcggcggac ggggggcaag ggtagccgcg cgctccgcgc ttttgcgcgg
     2281 gacgagggag ggccaggtct tcgcaagaac ctccgccacc ttgtcgggag tgtactcccc
     2341 tatccagtcc tcggggtcgg tcttgccgac gatcacggtg cgtatgccga ggtcgaaggc
     2401 cagcgccttg atctcgcgtt cgagcacgtc gtccagctct tcgagcacct tcacggaggc
     2461 gtgggagcgc aggaagtcgg ccacgagtcc cttcgggaga gggtgcacca cgccgagctt
     2521 gaggatatcc ggcttttccg ccgcgtcctc cagcacgtcg accagcgagc ggaagggaag
     2581 ccccgccgtg atgacgccct tcgacggatc gttcccctcg cgcagttcgt ggagcggcga
     2641 gccgtcggtc tcctgcgcca cttcggccag acgggcgatc gcgcgccgtt tcatgtcgag
     2701 tgcgcgcgtg gtgagcggaa tggacggccc tgcggaggag tcgaacgcgg gaacgctccc
     2761 gatcggacgg ggcgtccacc ctgcgaagga gaccttctcc ttgccgtggc agacgtgcgt
     2821 ggtcatccgg aggatgacgg gcatcttccg ctttctagag agcttcatcg cctcaaggta
     2881 gaacgtgtag gcctccgcag gcgaggcggg ttcgagcacg gggatgtagg cgaggcgggc
     2941 gatgtggcgg ttgtcctgtt cgttctgcga ggagttcgca ccggggtcgt cgccgagaat
     3001 ggcgaccagg ccgccgccca gctccagcag cgagagctg
//
LOCUS       DAOPKU010000369         3031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_36611_length_3031_cov_2.505376, whole genome shotgun sequence.
ACCESSION   DAOPKU010000369 DAOPKU010000000
VERSION     DAOPKU010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36611_length_3031_cov_2.505376"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PK849_10840"
     CDS             complement(<1..1075)
                     /locus_tag="PK849_10840"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPE66662.1"
                     /translation="MNEGENMDIRWNEPKVLHLFCLFCGIPLMGKSYILIPFFVFLYL
                     IWNRRNWLAANCRSAFRAVPEFRYLTFFLSGYGLFALIGFFRMPGSLYTRTDGPLYAA
                     IFAVLLIVGFVSGSWEEQLPVREKAKIFSMWIQAWGWIIGIDVLLKRMKFPYLDLGIV
                     NEINALSTLFLILAPLSLLSFFSKRGFLFRSFGFLSFCFFSWLLFFCTTSDVRYVYWL
                     VNLLVVYFFHRSESVRLVPVALFFFLAALMAGYSDEIVAFLKSIDLFQAPFGEGKVPF
                     FQKILNTRDAIWQGSLRAFGEHYWWGVGPANFHEYVTPYLLPLKQYFQRVPHFWQAHN
                     IFLHIFVVTGVWGGLCFLFSALVQ"
     gene            complement(1092..2129)
                     /locus_tag="PK849_10845"
     CDS             complement(1092..2129)
                     /locus_tag="PK849_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HPE66663.1"
                     /translation="MVNLFMPYTPYHLLLSFAIALSMPGELHYLILGESSPGNLKVFR
                     LLQEIYASAGITAFEMEGTFQKSNVKKFLIKRRNISRVDTLFKELPAIDRFFYFCDCH
                     VVTTYSAFLARKENPGIEFFFVEDGVGSYISSPRTSKNGVEKIADRLLFGKWHTDTVV
                     PGSFMESTGAWALFPDFLPSFFDEKKKFAVSREKLLSEFDRGLFSQKSGSLPDTARSL
                     VAVDFEHYTETDAYRDAVSSYIRRAEALGGNSVLKLHPSDRRQHVFYGADNIFTLPSH
                     LPVEVFYLFYGDSLRFVVGGLTTALLTAKWLVPDAEVVSIIPQSIVQSVHNAQEIFSV
                     FSACGIKVCTL"
     gene            complement(2222..>3031)
                     /locus_tag="PK849_10850"
     CDS             complement(2222..>3031)
                     /locus_tag="PK849_10850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE66664.1"
                     /translation="AVPYKVLSLDCHRGVSAARNLGMKEAAGDCVFFLDADDYIEKTC
                     LEKLYSLFCDDENVDVAFCGFRRVNVLGKVLQRYFPRKKYLNHAVSGTEALSLFWKSK
                     IDLHMTSCMVRRSFLFEKSLFFYEGCIYREDFEFFSRILFKARRVTSFPEELSSYVVD
                     KSSTTGKSEFMNKAAIHELSVFIRLRKMVGEMGNAGELGNLIEKFTLHGYIEVLERTL
                     KKGKQEEFRLMMKSESIRKSLAVSFRRDVLLFKPEYFLKVLHLYLCFIICR"
BASE COUNT          975 a          717 c          636 g          703 t
ORIGIN      
        1 cctgcaccag ggctgagaaa agaaagcaaa gcccccccca tacacctgtt acgacaaaaa
       61 tatggagaaa aatattatga gcctgccaga aatgaggaac tctttggaag tattgcttta
      121 aaggcagcaa gtatggtgtc acatattcgt gaaagtttgc aggaccgact ccccaccagt
      181 aatgttcccc aaatgcacgg agggatccct gccatatagc gtcgcgggtg ttgagtattt
      241 tctgaaagaa gggaactttt ccctcgccaa aaggagcctg gaacaaatca atagatttga
      301 gaaacgccac tatttcatcg ctgtaccccg ccatcaaggc cgcgaggaaa aaaaacagcg
      361 ccaccggaac aagcctgacg ctttcagaac ggtggaaaaa gtacaccacg agaaggttga
      421 ccaaccagta gacatatcgg acatcgcttg tagtgcagaa aaacagcagc caggaaaaaa
      481 aacagaacga cagaaaccca aagcttcgga aaagaaaacc tcgtttcgaa aagaaagaaa
      541 gcaacgagag gggggccaga atgagaaaca gtgtcgaaag agcgttgatt tcattaacta
      601 tgcccaaatc gagataagga aacttcatcc ttttcaaaag gacatcaatt ccgataatcc
      661 acccccaagc ctggatccac atggagaaaa ttttggcctt ttccctgacc ggcaattgct
      721 cttcccaact gccggaaacg aagccgacga tcaacagaac tgcaaagatc gcagcataaa
      781 gggggccatc cgttcgagtg tagaggctcc ccggcatgcg aaagaagcct atgagcgcga
      841 aaaggccata gccggaaagg aaaaacgtca gatagcgaaa ctcgggaaca gcccgaaaag
      901 cgctccggca attcgcggcc aaccaatttc tgcgattcca gatcaaatag agaaaaacaa
      961 aaaaggggat caatatatag gatttcccca tgagcggaat accgcaaaaa aggcaaaata
     1021 aatggagcac cttaggttca ttccatcgaa tatccatgtt ctctccttca ttcatcaaat
     1081 aatactctca attacagagt gcacactttt attccgcaag cggaaaagac gctgaatatt
     1141 tcctgtgcat tatgtacgct ctgcacaatg gattgaggaa taatggaaac cacttccgca
     1201 tccggaacaa gccatttcgc ggtaagaagc gccgttgtca aaccgccgac aacaaatcga
     1261 agagaatcac cgtaaaacag gtaaaatacc tctacaggca ggtgggatgg aagggtaaaa
     1321 atgttatccg caccataaaa gacgtgctgc ctcctatccg aagggtgcag tttgaggaca
     1381 gaattcccgc caagagcctc tgctctgcgt atgtaggacg aaacagcatc gcggtacgca
     1441 tccgtttcag tgtaatgttc gaaatcaacg gcaacgagag agcgggcagt gtcgggaaga
     1501 gatccacttt tttgagaaaa aagcccccgg tcaaactctg aaaggagttt ttctcttgag
     1561 acggcaaact tcttcttctc atcgaaaaag gaaggcaaga aatccggaaa gagtgcccag
     1621 gctcctgtgg attccataaa acttcctgga acgaccgtgt ctgtatgcca ttttccgaat
     1681 aagaggcggt cggctatttt ttcgacccca tttttggatg ttcgggggga agatatgtat
     1741 gagcccacac cgtcctccac gaaaaaaaat tcaatcccgg gattttcctt tcgggcaaga
     1801 aaggctgaat acgtcgtcac aacatggcaa tcgcaaaaat aaaagaaacg gtctatagca
     1861 ggcaactctt taaacaaagt gtctaccctg cttatatttc gtcgttttat taaaaatttc
     1921 tttacattac ttttttgaaa tgtcccttcc atttcgaatg cggtaatgcc cgctgaagca
     1981 taaatctcct gcaaaaggcg aaaaaccttt aaattaccgg gactgctctc accaaggatt
     2041 aaatagtgca gctcaccggg cattgaaagg gcgatagcaa atgagagcaa aagatgatat
     2101 ggtgtatatg gcataaataa attgaccatg ggctccactc cctggcgagg aattaaagtc
     2161 tccagaaatt atctacccca aaaaatgcct gttccagcag aattcataca gcatcttttt
     2221 gttaccggca gattataaag cataaatata gatgcaggac ttttaagaaa tattccggct
     2281 taaacaaaag aacatctctt cgaaaggaaa cggcaaggga tttccgaata ctctcagatt
     2341 tcatcattaa tcgaaattcc tcttgtttcc ccttttttag cgttctttcc agcacttcaa
     2401 tgtacccgtg aagagtaaat ttttcgataa ggttacccaa ttcccctgcg ttccccattt
     2461 ccccaaccat ctttcgaagg cggataaaga cagataattc atggatcgcc gccttattca
     2521 taaattcact tttcccagtc gtggaacttt tatccacgac ataggatgaa agctcctccg
     2581 gaaacgaagt tactctcctc gctttaaaaa ggattctgct aaaaaattca aaatcctctc
     2641 tgtaaatgca cccctcgtag aaaaagagag atttctcaaa aagaaatgaa cgtcttacca
     2701 tgcaggatgt catatgcaaa tctattttcg atttccagaa tagactgagc gcctccgttc
     2761 cggagaccgc atgattcaag tacttttttc ttggaaaata ccgttgcagg acttttccca
     2821 aaacattcac gcgtctaaaa ccacaaaagg ccacatccac attttcatcg tcgcagaaca
     2881 gggaatacag tttttcaaga cacgtttttt ctatgtaatc atccgcatca aggaaaaaaa
     2941 cgcagtctcc cgcagcttct ttcattccaa gattgcgggc ggctgagact cccctgtgac
     3001 agtccagaga aagcactttg taggggacag c
//
LOCUS       DAOPKU010000370         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37092_length_3003_cov_3.777815, whole genome shotgun sequence.
ACCESSION   DAOPKU010000370 DAOPKU010000000
VERSION     DAOPKU010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37092_length_3003_cov_3.777815"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1083
                     /locus_tag="PK849_10855"
     CDS             130..1083
                     /locus_tag="PK849_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HPE66665.1"
                     /translation="MKRITAVVCLVFLMALAPAAFAAYPEKPVRVVNYVGPGGLMDVT
                     SRKFVSIAAKYTDATFVVENKEGAGGLVGMQAVLEQPADGYTVFAATTSNIAKVLTSK
                     KDVNEFIWGFEWIAMLMQDPECLIAAKDNPLNTWEAVHKDAMEKNGAQLWTGPAAGGN
                     DHLLAMKVWDKSGMSAKWIPYSTGPEAMMALLSGQGVVYVGNAADIGGREGFSIIAVA
                     RKERLKKFPDAPTFIELGVEGLEEEAMWRGFAMKKGSPEEAMVWWEELLKKVAADPEW
                     KEFLEKDGIDVVDYGREKFNAQVQKDIDNFTVYLKKYGLIK"
     gene            1234..1788
                     /locus_tag="PK849_10860"
     CDS             1234..1788
                     /locus_tag="PK849_10860"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HPE66666.1"
                     /translation="METLLLFLSGADSFPVWILSFLAVIALTAFLTRGKHAPFRGRII
                     ICVSLLFLAFAFFLLSFGLEEIEAVGSSARTAPRLWAAGLAFFTLDQLRRTLLGKAPE
                     DPKTGDVQRVLLAAAIVAFSLWGMEIVGYLISSGGMILLLLLLFGERRLFILLSLTGG
                     WMLFSWFVFIRLLHLSLPAGIYFG"
     gene            1801..>3003
                     /locus_tag="PK849_10865"
     CDS             1801..>3003
                     /locus_tag="PK849_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPE66667.1"
                     /translation="MDLFSHLLDGLGALMGITPLLVIMAGVAIGILAGAMPGISPSMA
                     VALLLPFSFGMTPSMGLVLLCAIYLASNYGGSITAVMVNTPGTPSAVVTAFDGYPLTR
                     KGDAGTALGVSLVASVAGGFIGIIILVLFSAPLARMALKFWPAEYFALAILGLSTIAS
                     LGGGRWLESFMAVLLGLLLNTIGLDPLSGVSRFTFDVVRLYDGFSFIPVLIGLFALSE
                     VFSGIESGEVGIPPVQPDKERSPWPTVREYLKLKYSMIRAGVLGTVIGVFPGAGGTIA
                     SFIAYDVEKRLSKEPESFGKGAPAGVAAAEASNSASVGGALVPLLTLGIPGSSSAAVL
                     IGALMIHDLQPGPELFTRHPEVVYTLFSSLFVANIFLLFLGLLGARLWIKAALIPKRL
                     LYPMIFAFS"
BASE COUNT          540 a          808 c          895 g          760 t
ORIGIN      
        1 atggcctgac cttcgagctt ttttctcggt agttttacct aaagccgggt gtttcgttcc
       61 ggaccggaaa tgtgtttgtc gatggtacgg aacgcgtatg gtacttcaaa ttctcaggag
      121 gtgttcttga tgaaacggat taccgcggtt gtgtgtcttg tttttctgat ggcgctggct
      181 ccggcggcat ttgccgcata ccccgagaag cccgtccggg ttgtaaatta cgtaggtcct
      241 ggcggactga tggatgtgac gagcagaaag ttcgtctcca tcgcggcgaa atacacggat
      301 gcaactttcg ttgttgagaa caaggaaggc gccggcggac tcgtcggaat gcaggcggtt
      361 ttggagcagc ccgccgacgg gtacacggtg tttgcggcga cgacgtcgaa tatcgccaag
      421 gtgctgacct ccaaaaagga cgttaacgag tttatctggg gattcgagtg gatcgcgatg
      481 ctcatgcagg acccggagtg ccttatcgcg gccaaggaca accctctgaa cacgtgggaa
      541 gcggtgcaca aagacgcgat ggagaagaac ggcgcgcagc tttggacagg gccggctgcc
      601 ggaggtaacg accatctcct ggcgatgaag gtgtgggata agtcgggaat gagcgcgaag
      661 tggatcccgt acagtacggg gcctgaagcg atgatggcgc tgctcagcgg acagggcgtc
      721 gtgtatgtcg ggaatgcggc ggacataggc ggacgcgagg gcttttccat aattgccgtg
      781 gcacgaaaag agcggctcaa aaaattcccc gatgcgccga cttttatcga actcggggta
      841 gaaggtcttg aagaagaggc aatgtggcgc ggtttcgcca tgaagaaagg gtcccccgaa
      901 gaagccatgg tctggtggga agagcttctg aagaaagttg ccgccgaccc cgaatggaaa
      961 gagtttctcg agaaggatgg aatcgacgtc gtcgactacg gccgtgagaa gttcaacgca
     1021 caggtgcaga aagacatcga taattttacg gtctacctca agaagtacgg tctgattaag
     1081 taagtttttc actctttcgc atcaaatacg gaccaacaag tgcggggtat cgcccgagac
     1141 ggcggttccc cgcacttcat ccgccggtag tacggcggcg ggcttggtgg cgtacaataa
     1201 caatggagcc ggggtgctcc cggaggtggt tccatggaaa ctctgcttct ttttctcagc
     1261 ggggccgaca gtttccccgt ctggattctt tcgtttcttg ccgtcatagc gctgacggcc
     1321 ttcctgacac gtgggaagca cgcgccgttc agggggcgta taatcatctg cgtgtcgttg
     1381 cttttccttg cattcgcttt ctttcttctc tcattcggtc tcgaggagat cgaagccgtc
     1441 ggaagcagcg ccagaacggc gcccaggctc tgggctgcag gactggcttt ttttactctg
     1501 gatcagctgc gacgcacgct cctggggaag gctcctgagg atccgaagac cggtgacgta
     1561 caacgggttc tgctggctgc ggccattgtc gctttctctc tttgggggat ggagatcgtc
     1621 ggttacctga tctcctcggg cggaatgatt cttctccttc tgctgctctt tggagaacgc
     1681 cggttgttta tccttctttc actgacaggg ggctggatgc tcttctcctg gtttgtcttt
     1741 attcgcctgc tgcatctctc gcttcctgcg ggcatctatt tcggataggg ggaacgtcga
     1801 atggatcttt tctcccattt gcttgacgga ctcggagcgc tcatgggaat tactcctttg
     1861 cttgtaatta tggcgggcgt ggcgatcggc attcttgcag gcgccatgcc cggcatctct
     1921 ccctccatgg ccgtcgcgtt gcttctgcca ttttcctttg gaatgacccc ctcgatgggg
     1981 ctcgtcctgc tttgcgctat ctaccttgcc tccaactacg gcgggtcgat caccgccgtc
     2041 atggtgaaca cgccggggac gccctcggct gtggttaccg cctttgacgg ctatcctctg
     2101 acacgaaaag gcgatgcggg aaccgcgctc ggcgtttctc ttgtcgcttc tgttgccgga
     2161 ggatttatcg gaatcatcat cctggtgctc ttctcggccc ccttggcgcg tatggcgctg
     2221 aagttctggc ctgcggagta ctttgctctg gcaattctcg gactctccac gatagcctct
     2281 ttgggaggag gacgctggct tgagtccttc atggcggtac ttctggggct gctcctgaat
     2341 accatcgggc tcgaccctct cagcggtgtt agccgcttta cgttcgacgt ggtccgcttg
     2401 tacgacggtt tctccttcat tccggtgctg ataggccttt ttgctctcag cgaggtcttt
     2461 tccgggatcg aatcgggaga ggtcggtata ccccccgtgc agcccgacaa ggagcgttcc
     2521 ccctggccga cggtacgcga atacctgaag ctgaaatact ccatgattcg tgcaggggtt
     2581 ctgggtacgg tcatcggagt cttccccggg gcgggcggaa cgattgcctc cttcatcgcc
     2641 tacgacgtcg agaagcgcct cagtaaagaa cctgagagtt tcggaaaagg cgctcccgca
     2701 ggcgtcgccg ccgcagaggc ctccaacagc gcctcggtgg gcggggctct tgttccgctg
     2761 cttacattgg gcattccggg aagttcctca gccgccgtgc tcatcggcgc tctgatgatt
     2821 catgatctcc agccgggacc ggagcttttc acccgccatc ctgaggtggt gtacacgctc
     2881 ttctcgagcc tcttcgtggc gaatattttt cttctttttc tcggtttgct cggagcccgt
     2941 ctttggataa aggctgcgct catcccgaaa cgtctgctct accctatgat ttttgctttt
     3001 tca
//
LOCUS       DAOPKU010000371         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37096_length_3003_cov_3.447761, whole genome shotgun sequence.
ACCESSION   DAOPKU010000371 DAOPKU010000000
VERSION     DAOPKU010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37096_length_3003_cov_3.447761"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PK849_10870"
     CDS             complement(<1..1318)
                     /locus_tag="PK849_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPE66668.1"
                     /translation="MKVPPVAGTRFFPVNRGEMEERGWAELDFLFVSGDAYVDHPAFG
                     TAVIARVLEAAGFRVGILAQPDWRSRDAFLSMGRPRYAALVSAGNLDSMLNKLTASKK
                     SRSTDSYSPGGKAGMRPDRATIAYCSRVKECWKDLPVIIGGVEASLRRFAHYDYWSDE
                     VRRSILFDSQADLLVYGMGELQIRQIADLLSRGVPVGRLQDLRGTVYRTNEFPEIRGK
                     FVEIPSYEEVLSDKKRFAEAFRMQDAEQDAFHGKTVVQRHGASFIVQNPPSPPLSTEE
                     MDAVYALPYTRTYHPMYEAAGGVPAIAEVRFSLTSHRGCFGSCSFCAIHYHQGRIIQS
                     RSHDSLLREARILTELPDFKGYIHDVGGPTANFRIPACDQQLVRGACRNRQCLSPTPC
                     PKLRADHSDYIDLLRKLRALPKVKKVFIRSGIRFDYLMADKKGNFLE"
     gene            complement(1333..2775)
                     /locus_tag="PK849_10875"
     CDS             complement(1333..2775)
                     /locus_tag="PK849_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66669.1"
                     /translation="MKNMDNSGIPGKEERGGDRLCPECGSRVENSFRFCPGCGSFVPE
                     YASVRQVPGAQRKETEYEDDSEDDGVVSVACGPGGNEREVEGAFAYEQPRSNADRAGT
                     SFREFNAAIQDRRGRRDRKGGIFLLLLFFMFVAALGGGVYWFLQQAEQIPWENDVREK
                     PAADPTPVAQENRDPDLPPLATPGGAPADVTPALPLSPPVQADALEISRPTKGVVIGS
                     GVNLRGSHTVSSPVVGKVTTGNEVEVLESWTSDEGAEVVALVNVELVADDGKTAKIVR
                     GRGLSVISGPDSQGMLRVALPNDRSKKQYSVAANTMSNPHSWPWYKIRHGGGKEGWIF
                     GKFITVFNPRENTLSPTVLDRALHTFGSTKEALEGALGKPLKTASRKVTRSGVAADEL
                     TLTFNGVTAVLQERGGASEVKSLTLSSSKHSLDGGFAVGSDRLAVLSILGLPNVLDKG
                     SEVYRLDANSGIRIHYESYKVRTIHVGMLK"
BASE COUNT          558 a          965 c          901 g          579 t
ORIGIN      
        1 cctcaaggaa attgcccttc ttgtccgcca tgagataatc gaagcgaatg ccggagcgga
       61 tgaagacctt cttcaccttc ggaagcgcgc gcagcttccg gagcaggtcg atgtagtccg
      121 agtggtcggc gcggagtttc gggcaggggg tcggggagag gcactgcctg tttctgcacg
      181 cccccctgac gagctgctgg tcgcaggcgg ggatacgaaa gtttgcagtc gggccgccga
      241 cgtcgtggat gtaccccttg aagtcgggca gctcggtcag aatccgcgcc tcccggagca
      301 gggagtcgtg gctgcggctc tggatgatcc gcccctggtg gtagtggatg gcgcagaagg
      361 aacagcttcc gaagcagcct cgatggctgg tcaggctgaa gcgcacctcg gcgatcgcgg
      421 gaacgccccc cgccgcttcg tacatcgggt ggtaggtccg tgtgtacgga agcgcgtaga
      481 cagcgtccat ctcctccgtc gaaagcggcg gcgagggagg gttctgtact ataaacgacg
      541 cgccgtgacg ctgcaccaca gtcttgccgt ggaacgcgtc ctgctcggcg tcctgcatgc
      601 ggaaggcctc ggcgaaacgt ttcttgtcgg agagaacctc ttcgtaggag gggatctcca
      661 cgaacttgcc gcggatttca gggaactcgt tcgtcctgta gacggttccg cgaagatcct
      721 ggagccgtcc gacagggacg cccctggaga gcaggtcggc gatctgtcgt atctgaagct
      781 cccccatccc gtagaccagc agatccgcct ggctgtcgaa gagaatggaa cgccgcacct
      841 cgtcggacca gtagtcgtaa tgggcgaacc gccgaaggct cgcctctacg ccgccgataa
      901 tcacaggaag gtctttccag cactccttca ccctgctgca gtaggcgatc gtcgccctgt
      961 ccgggcgcat ccccgcctta ccgccgggag agtagctgtc ggtgctccgg ctcttcttcg
     1021 aggccgtcag tttgttgagc atcgagtcaa gattgcctgc ggagacgagc gccgcatagc
     1081 gcggtctccc catggagagg aacgcgtccc gggagcgcca gtcgggctgc gccagaatgc
     1141 ccacgcgaaa acccgcggcc tccagcacgc gggcgatcac cgcagtaccg aaggccggat
     1201 ggtcgacata ggcgtcgccg ctgacgaaga ggaaatccag ctcggcccag ccgcgttcct
     1261 ccatctcccc cctgttcacg gggaagaagc gcgtcccggc aacaggggga accttcatcg
     1321 ggggaaaagc gttcacttca gcataccaac atgaatcgtc ctgaccttgt agctttcgta
     1381 gtggatacgg atgccgctgt tcgcgtcgag acgatagact tcgctcccct tgtcgaggac
     1441 gttcggaagc cccagaatcg agaggacggc caagcggtcg ctcccaacgg cgaatccgcc
     1501 gtcgagagag tgtttcgagg aggagagcgt gagcgatttg acttccgacg cacccccccg
     1561 ctcctgcaac accgcggtca cgccgttgaa ggtcagcgtg agttcgtccg ccgcgacgcc
     1621 cgagcgagtg accttcctcg aggcggtttt cagcggttta ccgagcgcgc cttcaagcgc
     1681 ttcctttgtg gaaccgaaag tatgcagcgc acggtccaga actgtcggcg acagggtatt
     1741 ctcgcggggg ttgaagacag tgatgaactt gccgaaaatc cacccctcct tgcctccgcc
     1801 gtggcgaatc ttgtaccagg gccatgaatg cgggttgctc atcgtattcg cggccaccga
     1861 gtactgtttc ttcgaacggt cgttgggcag cgccacacgg agcattccct gcgaatccgg
     1921 cccggaaatc acggaaagcc cgcgcccccg gacgattttt gctgtcttgc cgtcgtccgc
     1981 gacaagctcg acgttgacca gggcgacgac ttccgccccc tcgtcgctcg tccacgactc
     2041 cagtacctcg acctcgttgc ccgtggtcac cttccccacg acggggctgc tcaccgtgtg
     2101 cgagccgcga aggttcacac cggagccgat gactaccccc ttcgtcggac gggagatttc
     2161 gagggcatcc gcctgcacgg ggggagacaa gggcagggcg ggagtcacat cggcgggcgc
     2221 ccctccgggc gtcgccaacg gcggcagatc aggatcgcgg ttctcctgcg ccacaggcgt
     2281 cgggtcagcc gcgggctttt cacgcacgtc gttctcccag ggaatctgct ccgcctgctg
     2341 aaggaaccag tagacgcctc cgccgagcgc cgccacgaac ataaaaaaga ggagaagcag
     2401 gaaaatgccg cccttcctgt ctcttctgcc tcggcgatcc tgaatggctg cgttgaactc
     2461 acgaaaactt gtgcctgccc tgtcggcgtt ggaacgaggc tgctcgtacg caaacgcccc
     2521 ctcgacctcc ctctcgtttc cgcccggtcc acacgctacg gagacgacgc catcatcttc
     2581 actatcgtct tcgtactccg tttctttgcg ctgtgcaccg ggaacctggc gtacagaggc
     2641 gtattcaggg acgaacgagc cgcatccggg gcagaagcga aagctgttct ccactctgct
     2701 cccgcattcc ggacaaaggc ggtcgccgcc ccgttcttct tttccgggaa tcccggagtt
     2761 gtccatattc ttcatgtaca ggaaacctcc tctcgaatcc acggaaaaac gcctttacag
     2821 agtataccgt gtaccatttc tattctatca tttccccgga ctgcgtttcc tccgtcaaat
     2881 accggaacat ggaggctgaa agactcagtc ggcctgcccc ttctgctgca ttttcgccca
     2941 cgagtccttc agcgcgaccg tcctgttgaa caccggccgg tcaggcgtcg agtcagcatc
     3001 cgc
//
LOCUS       DAOPKU010000372         2997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37208_length_2997_cov_3.269545, whole genome shotgun sequence.
ACCESSION   DAOPKU010000372 DAOPKU010000000
VERSION     DAOPKU010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37208_length_2997_cov_3.269545"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PK849_10880"
     CDS             <1..1137
                     /locus_tag="PK849_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPE66670.1"
                     /translation="FFLYCYWMRNLDPEYGLVSPNDASTMIAGQTSLRLGDQLLKPIL
                     DVIGEKKNILIVPHDLLYVFPYETMALREPDPLKNGHGVYLLDDNWIFAEIPSVFLLQ
                     KNKEILPTDPNNLSRLVIMANPETWPKKPEDGNKNTRFIETYGMDFREMHKLLLDRLF
                     VKDPYLQSIREMWCPSIKLLEGTEKEKDLITTVWKGFGVVDEYKGSDVSEKNLYDPKN
                     KVWDARYLHIACHGYDRGSIPDLQPGLALSPFEDKGNDSFAQMGELAAFRWRSELIAL
                     SACDTGLGELFIGDGMLGLSTVFLAGGAKGMVISRWRVSDESAPLLMEKMYSEIVAGK
                     TPVHALASAKLAIKRGDHPAPSDTRRTQRTPNDWAVFKFVGVPW"
     gene            1224..2765
                     /locus_tag="PK849_10885"
     CDS             1224..2765
                     /locus_tag="PK849_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66671.1"
                     /translation="MKKIFILSSCLLVLLNLFFALEANASADLLRERAKNLYSVQDYR
                     GALSLFDRLLEEVPGDGEALDYSAWCLRYLGDWKSAEERFLQALEAPDGALISWLHVG
                     LGEMYLGAGDEAKSAESFQKAMDTAPEDDELLLRSLKGIIWVKAFLGDRVEYEKALSL
                     LENKEKALAEEVAADTASILEEREKARAEKTEVAEEPTEPILVLDTVERQAKLLEDDE
                     LELEDELEEILAEVQDKDEPVSEDRDAAGEKESEQLEIVVLEEEEIVVPTPAAHEMPP
                     CESSGEVAQPDASATDPETRDAESPTPALGEAHAPDKAMEATPAPKQPVKKPATRKPV
                     SKPVQSGVLASPQQPVSEVRGVRLGSPLEEELKRLEAEGVKLDRESTVDKNGVLRFAL
                     VPKTSFFPSHILSDAASEFYHIEGFSGFVLKVQGMLKTKPQSRPLEWKKTVFEAMVAD
                     FSSQYGKPAILADQGTSSEAAWFIPDRRVLWVFADAGLDNTCSLQISYVDRRIQAGHL
                     ITILK"
BASE COUNT          781 a          651 c          831 g          734 t
ORIGIN      
        1 tttttcctct actgttactg gatgcgcaac ctcgaccctg aatatggtct cgtcagtccg
       61 aacgacgcca gtacgatgat tgctggacag acgtctctaa ggctaggcga tcagttgttg
      121 aagcctattc tggatgtaat aggagagaaa aagaacatcc ttattgttcc tcacgacctc
      181 ttgtatgtat tcccttatga gacgatggct cttcgggaac ctgatccttt gaaaaacggg
      241 cacggagtgt accttttaga cgacaactgg atatttgcgg aaattccaag tgtttttctc
      301 ttgcagaaaa acaaagagat tctgcccact gatccaaaca atctcagtcg cctcgtaatc
      361 atggccaatc cggagacctg gcccaagaag cctgaggatg ggaataaaaa tacaagattt
      421 atagagacat atggaatgga ctttagggaa atgcataagc tccttttgga taggcttttc
      481 gttaaagacc catatctcca aagtattcgc gagatgtggt gtccttcgat aaaactgctt
      541 gaaggaaccg aaaaagaaaa agatttgatt actacggtat ggaaaggctt tggtgttgtt
      601 gatgagtata aggggagtga tgtgtctgaa aaaaatttgt atgatcccaa aaacaaagtg
      661 tgggatgcgc ggtatctgca tattgcatgc catgggtatg acagggggag cattccggat
      721 ctacagccag gtttagcact ttcaccgttt gaagataaag gaaacgattc ttttgcgcaa
      781 atgggtgagc tcgccgcctt tcgatggcgg tccgaactta ttgccctttc cgcttgcgat
      841 acaggtctgg gggagctttt tataggtgat ggtatgcttg gcctgagtac ggtctttctt
      901 gctggcggag caaaggggat ggtaatttcc agatggagag tctctgatga aagcgctcct
      961 ctcttaatgg aaaaaatgta cagtgagatt gtcgccggaa agactcctgt ccatgctctt
     1021 gcttcagcga aacttgcaat aaagcgagga gatcatccag caccctccga tactcggaga
     1081 actcaaagga ctcccaatga ctgggctgtt tttaagttcg ttggtgttcc gtggtaaata
     1141 aatagcaatt gattcagttg gccatatttt taggaatacc tagtttttga tctaatttat
     1201 ttaaaagttt ggaggtggca tttatgaaaa agatttttat tctttcctct tgtttgcttg
     1261 ttttgctgaa tctttttttt gcgctcgaag ccaacgcttc cgccgacctt ctccgcgaac
     1321 gggctaaaaa cttgtatagc gttcaggact acagaggggc gctttctctg ttcgacagac
     1381 tgctcgaaga ggttcccgga gacggagaag ctctcgacta ttcggcatgg tgcttgcgct
     1441 accttggcga ttggaagagc gctgaggaac gttttctcca agcgcttgaa gcgcctgatg
     1501 gcgccctaat cagttggctg cacgtcggat tgggagaaat gtatctcggc gccggggacg
     1561 aagcaaagtc ggcggaatct ttccagaagg ctatggacac tgctccggaa gacgacgaac
     1621 tgcttcttcg ttcactgaag ggaatcatct gggtaaaagc atttcttggt gatcgggtag
     1681 agtacgaaaa ggcactctct ttacttgaaa acaaagagaa agctctagcg gaggaagtgg
     1741 cagccgatac cgcttcaata ctggaggaac gggaaaaggc ccgtgcggaa aaaacggaag
     1801 ttgctgagga gccgaccgaa ccgattctgg tgcttgacac cgtggaacgt caggcaaagc
     1861 tgcttgaaga tgatgagctg gagctggagg acgaactaga ggagatcctc gccgaagtcc
     1921 aagataaaga cgaaccggtg agcgaggata gagacgcggc cggggaaaaa gagagcgaac
     1981 agctcgaaat agtggttttg gaagaagagg agattgtagt cccgacgcct gcggcccatg
     2041 aaatgccccc ttgcgaatca tctggggagg ttgcgcaacc tgatgcatca gcaacggacc
     2101 ctgagactcg ggatgcagaa tcgccgactc cggctttggg agaggcgcat gctccggaca
     2161 aagcgatgga agccacgccg gctccgaaac agcccgttaa aaaaccggcg acgagaaaac
     2221 cggtttccaa gccggttcag agcggtgttc ttgcttctcc gcaacaaccg gtttccgagg
     2281 ttcggggggt acgcttgggc tcaccgctcg aagaggaact caaaaggctg gaagcggagg
     2341 gggtcaagct cgatagagaa agcacagtgg acaagaatgg cgtgctcaga ttcgcactcg
     2401 ttccaaagac cagtttcttc ccgtcgcata ttctttccga tgcggcttcg gagttctatc
     2461 acatagaagg attcagcgga tttgtgctga aggttcaagg tatgttgaag acaaaaccac
     2521 agtcgcgtcc gctcgaatgg aagaaaaccg tctttgaggc gatggtggcc gacttctcgt
     2581 cgcagtacgg caagccggca attcttgcgg atcagggaac ttcttcggag gcggcatggt
     2641 tcatccccga cagaagagtt ctttgggtat ttgcggatgc aggactggat aatacatgta
     2701 gtttgcagat atcgtatgtt gacagaagga tccaggctgg acatctgatc actattctta
     2761 agtaagcgcg cggaaaaatt cgggggatgt cgcccttcca gaggggacat ccccgccttt
     2821 tactgaccag ccggaaggaa tgagtacgtt aatgggaaaa gtaaaccagc gatggcggcg
     2881 gaacaaactc gtctgcatag tctgcattct gattctgtgt cattgtatct gcggggccgc
     2941 gtttgcgaag acgcctgatg aaaagcggct tgcagatgcc attgtggata tccaagc
//
LOCUS       DAOPKU010000373         2994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37243_length_2994_cov_4.838380, whole genome shotgun sequence.
ACCESSION   DAOPKU010000373 DAOPKU010000000
VERSION     DAOPKU010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37243_length_2994_cov_4.838380"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PK849_10890"
     CDS             complement(<1..506)
                     /locus_tag="PK849_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE66672.1"
                     /translation="MNEEQRTILLVEDTRTNALAVERQLRKAGWGVLHAQTGEEAIRM
                     VCDEGAEIDLILMDIELGSGMDGSQTAREILKRKELPVVFLSSYTEAEVLERIEKIAS
                     YGYVAKNSGGAVLDASIKMAFRLHEANMRTARERNILRDVLESTLSGFWEMYFKSDAA
                     FLSPKFRNL"
     gene            complement(503..>2994)
                     /locus_tag="PK849_10895"
     CDS             complement(503..>2994)
                     /locus_tag="PK849_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66673.1"
                     /translation="RHGGVFMGSMLEQHILRSPLIGYSFRRIILDDSGKPVDYEFLEV
                     NEAFERFTGLARADILGRTASKVVPGIESASFDWLGVYGEVALHGGSKEFEQYSELLG
                     RWFRVHAYSDRKYYFTVLFLDVTENKKQAEELADEAFRRSLLMEQSRDGIVILDENGG
                     VYEANRKFAEMLGRPFESIRRLTVFDWEFLHPRERLIDMLRSVDEKGDHFETQHRRRD
                     GSVFDVEISTNAARFGGRKLIFCVCRDVTERKRAEKELRETLANARILQREAESANRA
                     KTTFLANMSHEIRTPLNGVIGFLGLLADTPLDAVQREYIEYIDTSARILLDILSNVLD
                     ISKIEAERLELHAVPSDIRRTVEHSIAPLRPAAAEKRIALSANVDDNVPTLATFDPVR
                     LEQVLVNLTNNAVKFTERGSVTLSVSFDPLKKNEGALTFRVTDTGIGIPPEERKRVFE
                     PFYQADNSNTRKYGGIGLGLSISQRILQMMGGSLEIHSVPGEGSSFFFTLRLAYDAEE
                     ARSPAATPLPEEKRGFPHSRRKDTPVILLAEDERLNMKMLTIMLSKLAPFSSVVQAED
                     GEKAVALFREHRPDLVLMDLQMPVIDGLQATAEIRAIEAKECREKERCCIVALTADVL
                     PETRGECLAGGMDDYLSKPVRREEIMAVLERCFGMNGGGERDFRDAPAEDAAWNRPLM
                     LDVLDGDEALADEIASEFLKETPQMLAALSDALAERDMEKLRRRAHTIKGIAGNVGGE
                     ALRSAAFDVEKTARLGDREKAVLLVVRLKDEFERLRKAMDNHLKTRTSLEAKTETNPQ
                     PEETPAVADEERRRNAPHKSTTDTATFQGEQ"
BASE COUNT          510 a          908 c          887 g          689 t
ORIGIN      
        1 agattccgga acttgggact cagaaacgcc gcgtcgcttt tgaagtacat ctcccagaaa
       61 ccggagaggg tggactcaag gacgtctctc aggatgtttc gctcgcgcgc cgtccgcatg
      121 ttcgcctcgt ggagcctgaa ggccatcttg atggaggcgt cgagcacggc ccctcccgag
      181 tttttggcga cgtagccgta cgaggcgatc ttttcgatcc gctccagcac ctccgcctcg
      241 gtgtacgagg agaggaaaac gacgggaagc tctttccgct tcaaaatctc acgggcggtt
      301 tgcgacccgt ccatgccgga gccgagttcg atgtccatta ggatcaggtc gatctccgct
      361 ccctcgtcgc acaccatgcg gatggcctct tcgcccgtct gcgcatggag cacgccccat
      421 ccggccttgc ggagctgccg ttccaccgca agggcgttgg tccgcgtgtc ctcgacgagg
      481 agaatcgttc tttgctcttc gttcattgtt ccccctgaaa agttgcggtg tcagtagtag
      541 atttgtgcgg cgcgttccgg cggcgttctt catcggctac cgcaggcgtt tcttctggtt
      601 ggggatttgt ttcggtcttc gcttccaagg aagtccgcgt cttcagatgg ttgtccattg
      661 ccttccgcaa ccgctcgaat tcgtccttca accttacaac aagaaggacg gctttctccc
      721 gatcccccag gcgcgccgtt ttttccacgt cgaaggcggc ggagcgcaga gcctctccgc
      781 ctacatttcc ggcgatcccc ttgatcgtat gggcgcgcct tctgagtttc tccatatctc
      841 tctccgccag cgcatcgctg agagcggcca gcatttgcgg cgtttccttc aaaaactccg
      901 acgcaatttc atcggccagc gcttcgtcgc cgtcgagcac atccagcatg agcggtctgt
      961 tccaggccgc gtcctcggcg ggtgcgtccc ggaagtctcg ttctccgcct ccattcattc
     1021 cgaagcatcg ttcgaggacg gccatgatct cttctctgcg tacgggtttc gacagatagt
     1081 cgtccatacc gcccgcgaga cactcccctc tggtttccgg cagaacgtcc gctgtgaggg
     1141 cgacgatgca gcagcgctct ttttcgcggc attccttcgc ttcaatcgcg cgtatctccg
     1201 ccgttgcttg caagccgtcg atcactggca tctgaagatc catcaggacg aggtccggcc
     1261 ggtgttcgcg gaagagggcg actgcttttt ctccgtcttc ggcctgtact acggaggaga
     1321 aaggagcaag cttcgacagc atgatggtga gcatcttcat attcagccgt tcgtcctcgg
     1381 cgagcaggat caccggggtg tcttttctcc gcgagtgggg aaatccccgt ttttcttccg
     1441 gaaggggcgt cgccgccgga gagcgcgctt cctccgcgtc gtatgccagc cgcagcgtga
     1501 agaagaagct gctcccctct ccggggacgc tgtgtatttc gagcgaaccc cccatcatct
     1561 gcagaatacg ttggctgatg gagaggccga gcccgatccc tccgtacttg cgcgtattgg
     1621 agttgtcggc ctggtagaag ggttcgaaga cgcgttttcg ctcttccgga gggattccga
     1681 tgccagtatc cgtcacgcgg aaggtgaggg cgccttcgtt tttcttcaga ggatcgaagc
     1741 tgaccgagag cgtgacgctt ccccgctccg tgaatttgac cgcgttgttc gtcagattga
     1801 cgagcacctg ctccagacgc acggggtcga aggtcgccag cgtaggaacg ttgtcgtcca
     1861 cgttcgccga gagcgcaatc cttttttcgg cggctgcggg gcgaagggga gcgatggagt
     1921 gttccaccgt cctgcggatg tcggaaggaa ccgcatgtag ctcgagccgc tccgcctcga
     1981 tcttcgagat gtcgagcacg ttgctcagga tgtcgagcag gatgcgcgcg gaagtgtcga
     2041 tgtactcgat gtactccctc tgtacggcgt cgagcggcgt gtcggcgagc agtccgagaa
     2101 atccgatgac gccgttgagc ggcgtgcgta tctcgtggct catattcgca agaaaggtcg
     2161 ttttggcccg gttggcggac tctgcctccc gctgaaggat gcgcgcgttg gccagcgttt
     2221 cgcggagctc tttctcggcg cgcttgcgct ccgtgacgtc gcggcagacg cagaagatca
     2281 gcttccggcc gccgaacctt gcggcgttgg tgctgatttc gacgtcgaaa acgctgccgt
     2341 ccctgcgccg gtgctgtgtt tcaaagtggt cgcccttctc gtcgacgctc cgcagcatgt
     2401 cgataaggcg ttcccggggg tgcagaaact cccagtcgaa gaccgtgagg cgtcgtatgg
     2461 attcgaaggg gcgcccgagc atttcggcga attttctgtt ggcttcgtac actccgccgt
     2521 tctcgtcgag gatgacgatc ccgtctctgg actgctccat gaggagactc ctccggaacg
     2581 cttcgtccgc gagttcctcc gcctgttttt tattttccgt gacatcaagg aaaaggacgg
     2641 tgaagtagta cttccggtcg gagtaggcgt gcacgcgaaa ccatctgccg aggagctccg
     2701 agtactgctc gaactctttt gaaccgccgt gcagcgcaac ctctccgtac acgccgagcc
     2761 agtcgaagga ggcgctttcg atgccgggaa cgaccttgct tgcagtgcgc cccagaatat
     2821 cggcgcgcgc aagcccggtg aatctctcga aggcctcgtt cacctccaga aattcgtagt
     2881 cgaccggctt gcccgaatcg tcgaggatga tcctgcgaaa agagtacccg atcaaggggc
     2941 tgcgcagtat gtgctgttcg agcatcgaac ccatgaatac acctccgtgc cgcg
//
LOCUS       DAOPKU010000374         2994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37246_length_2994_cov_4.401157, whole genome shotgun sequence.
ACCESSION   DAOPKU010000374 DAOPKU010000000
VERSION     DAOPKU010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37246_length_2994_cov_4.401157"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PK849_10900"
     CDS             complement(<1..363)
                     /locus_tag="PK849_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPE66674.1"
                     /translation="MKNAESRSIGVVFDFNGTLFWDTEYQERSWDTYLAKYGVELTKE
                     EKLTRIHGRNGKDSFEYIFKRELGREEVARLIEEKEEYYREECEQQEVALAPGAVPLI
                     EDLLERGVSIAIATAAGKT"
     gene            complement(408..1232)
                     /locus_tag="PK849_10905"
     CDS             complement(408..1232)
                     /locus_tag="PK849_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPE66675.1"
                     /translation="MSLCYMNGTFLPKEKAMLPVSDYIILRGVGVFESISTFRKRPLM
                     LTPHLERLARSAESASITLPLPIEEIKAVVQEGLSRMPEDCLIRPYITGGDVFISGAF
                     PASRLFILFEKVQKPAPEVYSNGVLLLPVDGGRHIPGVKSIDYMFSYSGYAKRGDAYE
                     ILYCPDGEITEAAHSSFFLYTGGKLVTAPLSRVLKGTMRDIILQLASEKGMKIEERCP
                     LLSELAAAEEAFITGSVKEVVPVVKIGDQVIGSGEPGPVTKMLHYTMLEEIVRWLE"
     gene            1337..>2994
                     /locus_tag="PK849_10910"
     CDS             1337..>2994
                     /locus_tag="PK849_10910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE66676.1"
                     /translation="MSKSVFRCFAHRLFFIALLLFLPRGEAFAQDEGYRVLLLNSYHS
                     NFVWTAEVTDGIRQTLLSSGSEVEFLTEYMDTKRGFSVDALKAFSGYMERKYSGRSFD
                     LLICSDDDALVFLRRMGKRLFPDVPVIFCGVNSTSLYEPEAYPHITGLFENVDISGTA
                     ELALRLFPRTSALALVSDLSTTGMTVISQARSELAFLGDRVRIHDLFAFSEEELQDAL
                     SALPPETVLLMLVYFQDEEGHFISPSRSVALVKDACVFPLFGLWSMMPEAGAFGGSVL
                     QARRHGEKAAEIALRVLAGVPPSAIPAETDTEYVPMFDFGELKRFRLSRSDLGQSSVV
                     LNEPERISSGSWKIIIINAVVVLASIGYVALLLANERRRKRAAKEIALRKSMWEGLFR
                     NAPDAYVVFGDDNTVLNVNEKFCEVFGYEYSEAIGRDIDSLVAKGTDAEEEAAEISRK
                     VLNGEAVYSEGVRRKKDGNTIHVGIQGVLFPVPEGCALGYGIYSDISERRRNEEEMNR
                     RLLSEAAISSISARLLKESGLIGIPPSLEEMASFTGAKEGFLIE"
BASE COUNT          588 a          841 c          891 g          674 t
ORIGIN      
        1 ggtcttcccc gccgcggtgg cgatcgcgat ggagacgccg cgttccagca gatcctcgat
       61 gagcggcacg gctccgggcg ccagcgcgac ctcctgctgt tcacactcct cgcggtagta
      121 ctcttccttc tcttctatga ggcgggcgac ttcctccctg ccgagttctc ttttgaagat
      181 atactcgaag gaatccttcc cgtttctgcc gtgaatacgg gtcagcttct cctctttggt
      241 gagttcgacg ccgtacttgg caaggtacgt gtcccatgag cgctcctggt actccgtatc
      301 ccagaagagc gtgccgttga agtcgaagac gacgccgatg ctgcggctct ccgcgttttt
      361 catcgctctt gttcagcctc ctcgttgttc ctgctgcgcc ggaagtgtta ctccagccag
      421 cggacgatct cctcaagcat cgtgtagtga agcatctttg tcacagggcc gggctctcct
      481 gagccgataa cctgatcgcc tatcttcacc acgggaacga cctccttcac cgagccggtg
      541 atgaacgcct cttccgcggc ggcaagctcc gaaaggagag ggcaccgctc ctcgatcttc
      601 atcccctttt ccgaggcgag ctggagtatg atatcccgca tggtcccctt gagtacccga
      661 gagagggggg ctgtaacgag ttttcctcct gtgtacagaa agaacgaact gtgcgccgcc
      721 tccgtgatct cgccgtcggg gcagtagagt atctcgtagg cgtcgccgcg tttggcgtac
      781 cccgagtagg agaacatgta gtcgatgctc ttcacccccg gaatgtgccg gccgccgtcg
      841 acagggagga ggagcacccc gttggagtag acctcgggcg cgggcttttg gaccttctcg
      901 aagaggatga agagcctgga tgcggggaaa gcgccggata tgaagacgtc gccgccggta
      961 atgtatgggc ggatcaggca gtcctcaggc atcctggaga gtccttcctg gacgactgct
     1021 tttatctctt cgatcggaag cgggagcgtt atcgacgcgc tctccgcgga acgcgcgagg
     1081 cgctcgagat ggggagtcag catcagcgga cgcttccgga aggtgctgat tgattcaaag
     1141 acgccgacac cgcggagaat gatgtaatcg gataccggaa gcatcgcctt ctccttcggc
     1201 aggaacgttc cgttcatata gcacagggac attgaaaatc acctcgaaat ttcggtcttg
     1261 gaacctttgc agtataaggg tatcatagag aaaatgcacg gggtttttcg acgaaagatt
     1321 tgaagggaga gcggttatgt cgaaatcggt gttccgatgc tttgcccaca ggctcttttt
     1381 catcgcgctc cttcttttcc ttccccgcgg agaggcgttt gcgcaggacg aagggtatcg
     1441 cgtgctgctg ctcaactcct atcacagcaa cttcgtctgg actgccgaag tcaccgacgg
     1501 aatccggcag acgctccttt cgagcggctc cgaggtcgag tttttaaccg aatacatgga
     1561 taccaaaaga ggtttttcag tggacgctct gaaggctttt tccgggtata tggagagaaa
     1621 atactccggc aggagcttcg acttgctcat ctgttccgac gacgacgcgc tcgtttttct
     1681 tcgcaggatg gggaagcgcc ttttcccgga tgttcccgta atcttctgcg gcgtgaacag
     1741 cacttcgctg tacgaaccgg aggcctatcc gcacatcacc ggtctgttcg agaacgtcga
     1801 tatctcgggt accgccgagc ttgcccttcg tcttttcccc cgcaccagcg ctcttgcgct
     1861 cgtgagcgat ctctccacca cgggaatgac ggtgatcagt caggcgcgga gcgaacttgc
     1921 gtttttgggg gatcgcgtcc gaatccacga ccttttcgca ttctcggagg aagagctcca
     1981 ggatgcgctc tccgctcttc cgcccgagac agtgctgctc atgctggtat attttcagga
     2041 cgaggagggg catttcatct ctccctcccg cagcgtcgct ctcgtaaaag atgcgtgcgt
     2101 ttttccgctc ttcggactct ggagcatgat gcccgaggct ggagcattcg gcggcagcgt
     2161 tctgcaggcc cgccgtcacg gagaaaaggc cgcggagatt gcgcttcgcg tgctcgcggg
     2221 cgtccccccc tcggctatcc ccgctgaaac cgatacggag tacgtgccga tgttcgactt
     2281 cggagagctg aagcgcttcc ggctctcgcg aagtgatctg gggcagagtt cggtggtgct
     2341 caacgagccg gagcgtattt cttcggggag ctggaaaatc atcatcatca acgctgtcgt
     2401 tgtactcgcg tccatagggt atgtcgcgct gctgctggcc aacgaacgca ggcggaagag
     2461 agcggcaaaa gagatcgcgc tccggaaatc catgtgggag gggctcttca ggaacgctcc
     2521 ggacgcatac gttgtcttcg gagacgacaa cacagtcctg aatgtaaacg agaaattctg
     2581 cgaggtcttc ggttacgaat atagcgaggc gatcggacga gatatcgaca gcctggtcgc
     2641 aaaggggacg gatgcggaag aggaagcggc ggagatctcc cggaaggtcc tgaacggcga
     2701 ggcggtctat tccgaaggag tgcgaagaaa gaaggacgga aacactattc atgtgggtat
     2761 tcagggggtg ctcttccccg tgcccgaggg ctgcgcgctg ggttacggta tttacagcga
     2821 catctcggag cggcgccgca atgaagagga aatgaaccgg cgtcttctct ccgaggccgc
     2881 aatctcctcc atttccgcgc gcctcctgaa agagagcgga cttataggca ttcccccgtc
     2941 gcttgaagag atggcttcgt ttacgggggc gaaggaagga tttcttatcg aatt
//
LOCUS       DAOPKU010000375         2991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37307_length_2991_cov_5.714237, whole genome shotgun sequence.
ACCESSION   DAOPKU010000375 DAOPKU010000000
VERSION     DAOPKU010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37307_length_2991_cov_5.714237"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..759
                     /locus_tag="PK849_10915"
     CDS             454..759
                     /locus_tag="PK849_10915"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPE66677.1"
                     /translation="MTTLLIERDVFAVDVSFSEDALSVSLDDGRILSVPLAWYPRLLC
                     GTPEERECFELIGDGEGIYWPDLDEDISVEGLLAGRRSSESSRSLSRWKERRAAAGI"
     gene            complement(1083..1331)
                     /locus_tag="PK849_10920"
     CDS             complement(1083..1331)
                     /locus_tag="PK849_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPE66678.1"
                     /translation="MDKCFGRAVDNSMERMKARLKTPEGRAEYGKRKGVVEPVFGIIK
                     EVLKFRQFLLRGMKKAKGEWTLACMAYNLKRMHIMQTS"
     gene            complement(1283..1618)
                     /locus_tag="PK849_10925"
     CDS             complement(1283..1618)
                     /locus_tag="PK849_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66679.1"
                     /translation="MEKEEYDAKMAAREKQEKATGKKRGGKKPAEAKPGPADKDQVNF
                     TDDESRIMPVSGGGFEQAYNARAAVDNESRLIVAVDLGRSFVKKPRGSHARLWTNVSG
                     VLWTTRWSA"
     gene            1913..>2991
                     /gene="istA"
                     /locus_tag="PK849_10930"
     CDS             1913..>2991
                     /gene="istA"
                     /locus_tag="PK849_10930"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HPE66680.1"
                     /translation="MSDFLTIVRAVYSGLSQRKVAATHGVSRNTVSLYLRKARDQGWL
                     TLKDLDAVDDTTVTQSLPQIAAPSRDATFKMPDFDYVHAELAKAHVTLKLLWEEYVEQ
                     CRQSNERFYMETQFRRYYHLHAKVHKATIRLEHKPAFSLEVDWAGTRIAFFDAESGKM
                     SQASLFVAVLPCSGIIYVEPFRDEKLPSWIAGHVHAFQYLGGVPKTLVPDNLKSGVKR
                     PNFYEPYLNKTYQEMAAYYGTVVLPARVRKPRDKASVENAVLISSRKIIATLRNVQIL
                     SFQDLQQHVRAALEHVNSAPLTGKSESRWTSFLAEEKEFLLPLPESPYELAQWGKAKV
                     QMNCHILCQRKFYSVPFEYLGEDVDV"
BASE COUNT          631 a          908 c          750 g          702 t
ORIGIN      
        1 gtcttcaaat ttttaccagt atctaagaac cagcaaactg ccgggcagtc gaatgtcctt
       61 acagtgcctc caaaaattca tttccaggtc atacaaagct ccaaagaccc gccataatta
      121 tccggagctt cttcgtggta gaatagtttc gtcgctacta cgcgccgttt tttgggagcc
      181 cccttctctt tccagttcca ggcgcagagc acatttacat gaagccagtt tagaagggag
      241 aaatcaatgc cgacatcggc ccgtacaaac tcttcttcta ctaggcggag gggaatgaac
      301 ctccttacat ccacgttcgt cgtgacagag caacggcaaa attctggctc gatccggtca
      361 ggattgcgac atcaaggcga ttcaccgact ccgatcttcg ggctctacaa aaacttgtgg
      421 aagaaaacga agtcgaaatt atggaggcat ggaatgacta ctttgttgat tgaacgagat
      481 gtctttgcgg tagatgtctc tttttctgaa gacgctttga gcgtttccct cgatgacggc
      541 aggattctct ctgttccgct cgcttggtat ccccggcttc tctgcggaac tccggaagag
      601 cgcgaatgct tcgagctgat cggcgacggc gaaggaatct actggccgga cctcgacgag
      661 gatataagcg tcgaagggct acttgcgggc cgccgctcct ccgagtcttc gcgttcgctg
      721 tcccgctgga aagaaagacg tgcagcggcg ggaatttgaa cctacggtag tgaacctctt
      781 aagggaagac acgagaggga ttttctcctc tcttgttata ttgcttgaaa agacatactc
      841 ctttcgcatc aaatcctatc ctctgaagct ggattttacg cattctaaca caagaagcat
      901 gtgcatcgcg ctcttcgctg acgcacgagc acaagcgcga ctgactagga gtctgtcgga
      961 ctggcctccg aaactccgta tcataagcgc catcgactcc ggatttcacg acgtccggat
     1021 acgaaagcac cgttttcaac gctccgtggg gtgaaatccc gccctccggg gcgtttttcc
     1081 ttttacgacg tctgcatgat gtgcattctc ttcaaattat acgccatgca cgccagcgtc
     1141 cattctccct tcgctttctt cattcctcga aggagaaact gcctgaactt caaaacttcc
     1201 ttgatgattc cgaagacggg ctcgaccacg cctttgcgct tcccgtactc cgctcttccc
     1261 tcgggcgttt tcaacctggc cttcatgcgc tccatcgagt tgtccacagc acgcccgaaa
     1321 catttgtcca cagtcgggcg tgactccccc tcggcttctt cacaaaactc cgaccgaggt
     1381 ccaccgccac gatcagcctg ctttcgttgt ccaccgcagc ccgggcgttg tacgcctgtt
     1441 cgaagcctcc tccggatacg ggcattatcc gcgattcgtc gtcggtgaag ttcacctgat
     1501 ccttgtccgc ggggccgggt ttcgcttcgg ccggcttctt gcctccgcgc ttctttccgg
     1561 tcgctttctc ctgtttctcc ctggcggcca tcttcgcgtc gtactcttcc ttttccatgc
     1621 ggtactgctc ggccgcacgg cgttcgatct ccttgatggc ttcttctatc aggtcgagac
     1681 gctccttccg ccgccgtcat ctctcctttc accactctgt ccagaatcac cgcttttgtc
     1741 gtttctttca ggcttggaaa acacggcgaa aatgagccgc ccgcgcggag tgttagagcc
     1801 attggcgcgg acctgaagag ccacccgacg cgccgacact gagccggtct cccgaactcc
     1861 tgtaagatcg aagtaccgcc ggaaagcgga attcatctca ggagggatta cattgtcaga
     1921 ttttctcacc atagttcggg ccgtttacag cggcctgagc cagcgcaagg tcgccgccac
     1981 gcacggggtg tccagaaaca cggtgtcgct gtatctgcga aaggcacggg atcagggctg
     2041 gctcaccctg aaggatctgg acgcagtgga cgacaccacc gttactcaga gtttgccgca
     2101 aatcgcggcg ccgtcccgcg acgcgacctt caagatgccc gacttcgact atgtgcatgc
     2161 tgaactggcg aaggcgcacg tcaccctcaa gctgctctgg gaggagtacg tcgaacaatg
     2221 ccgtcaaagc aacgaacgct tttacatgga gacgcaattc cgccggtact accacctgca
     2281 cgccaaggtg cacaaggcaa ccatccgtct ggagcacaaa cccgcgttct cgctcgaagt
     2341 ggactgggcc ggcacaagga tcgcgttctt cgacgccgag agcgggaaga tgtctcaggc
     2401 gtcgttgttc gtcgccgttc tgccgtgtag cgggatcatc tatgtcgaac cgttccgcga
     2461 cgaaaagctg ccgtcctgga tcgccggtca tgtccacgcc ttccaatatc tcggtggtgt
     2521 ccccaagacg cttgttccgg acaatctcaa aagcggcgtc aagcggccca atttctacga
     2581 gccctatctg aacaagacgt atcaggagat ggcggcctat tacggaacgg tcgtactgcc
     2641 cgcgcgcgtc cgcaaaccca gggataaagc gtccgtggag aacgccgtcc tgatttcgtc
     2701 ccgaaaaatt atcgccactc tccgcaatgt tcagattctc tccttccagg atctgcagca
     2761 gcacgtacgt gctgctctgg aacatgtcaa ctccgccccc ttgaccggga agagcgagag
     2821 ccgctggaca tccttcctcg ccgaagaaaa agagttcctg ctcccgcttc ccgaatcccc
     2881 ctacgaactg gcacagtggg ggaaagccaa ggtccagatg aactgtcaca ttctctgtca
     2941 acgcaaattc tactccgttc cgttcgaata cctgggagaa gatgtcgacg t
//
LOCUS       DAOPKU010000376         2983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37453_length_2983_cov_6.004781, whole genome shotgun sequence.
ACCESSION   DAOPKU010000376 DAOPKU010000000
VERSION     DAOPKU010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37453_length_2983_cov_6.004781"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PK849_10935"
     CDS             <1..326
                     /locus_tag="PK849_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HPE66681.1"
                     /translation="SVDSIPTSANQEDHVSMGAFSARKALMILDNARKVLAIELFAAS
                     QGLDFSRLLKPGAGTVAAHDCVRSVVPYLKHDEYLHPLIERVEALLSRGAVVRAVEEA
                     IGPLN"
     gene            456..>2983
                     /locus_tag="PK849_10940"
     CDS             456..>2983
                     /locus_tag="PK849_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPE66682.1"
                     /translation="MTDIMRNDDLFPQKNDMPFVNASRDIPRIICLGASAGGLEALER
                     FFRHLPEDVQDIFVIVQHLSPDHKSIMAELLHRYTSLPVVVVDDGMPPQPGRIHLIPP
                     GKEMVVENGAFRLRDRVLREPHLPINVFLQSMAREYGASCVAVILSGTGSDGAQGIKA
                     VHAAGGAVFAQDVDSSRFDGMPRNAALTCVVDFVGSPEQIVSALFKTAGDETGEPDTN
                     EERERLCEKDALEPKQLIFRKLSDAFGIDFSQYKDGTILRRLDRRMMMRAVADMSEYA
                     ELLEGDSEEVDHLYRDLLIDVTSFFRNPEAFSYLAETALPDLLAHHPENEEFRVWSVA
                     CASGEEAYSLAMILDEVMANRSQKRSVRIFATDIHKSSLKTASAGYYSNEKLANVSTA
                     RREKYFYRTGADGWTVRPNIRRMITFAPHNILSDPPFLHMHLISCRNMLIYLEKSAQI
                     ETLTRFSAALEQQGILFLGSSESVLDTQSEFLTVDSRLKIYRKQGTITSRPLYSLLPE
                     KKKATAGSERGSSKMPLSSLYPLLLERCMPTGFLVNSKGDLLHTFGGAGKYLQIIGPV
                     RTDIGSILHGNLRVAVMTAIERSRKNRTPVRFSDVSHPLPGAPLNTVEVSVEPLEERT
                     DSRSEEGNLFFFIRLEEMKPSLAAKAESFSVDEAAKARIEELERSLSEARENLQTIVE
                     ELETSNEELQATNEELLASNEELQSSNEELQSVNEEMHSLNAEYQEKNEQLIDLNNDL
                     QNLMSCTDIGTIFLDGELRIRRFTPAINRVFFLRDQDIGRPIEEMRSLVESGHDVVAS
                     IRRVLREGKVEEMEVTNREGVPMLQRIHPYVDIRGTVSGAVLTFV"
BASE COUNT          627 a          869 c          884 g          603 t
ORIGIN      
        1 cctccgtcga ctccatccct acctcggcca atcaggagga ccacgtgtcg atgggcgcgt
       61 tctcggcgcg aaaggcgctc atgatcctgg acaacgcgcg gaaggtgctc gccatagaac
      121 tcttcgccgc ctcccagggg ctcgacttta gccgtctgtt gaaaccgggc gcgggaacgg
      181 tcgccgcgca cgactgcgtc aggagcgtcg tcccctacct gaagcacgac gagtacctcc
      241 atccgctcat cgagcgcgtg gaggcgcttc tgagccgcgg cgcggtggtc cgggcggtgg
      301 aagaggcaat cggaccgctg aactgacaaa agacggaatc tcgaggggct cgccttcggc
      361 gggcctctcg ccgtatgatc agctctcctt cctcctgcga agacgggtat gctatactct
      421 ttttcagtac ataccgcgaa acagggggga tttcaatgac tgatatcatg cgaaatgatg
      481 atctcttccc gcagaaaaac gacatgcctt tcgtgaatgc atcccgggat atcccccgca
      541 tcatctgtct cggcgcctcg gccggaggcc tggaggctct tgagcgattc ttccgccatt
      601 tgccggagga cgttcaggat atcttcgtca tcgtccagca tctttctccg gaccacaaga
      661 gcatcatggc ggaactgctc caccggtaca cgtctcttcc ggtagttgtt gttgacgacg
      721 gaatgccgcc gcaaccggga cgtatacacc tgattccgcc gggcaaagag atggtggtcg
      781 agaacggggc gtttcgtttg cgggacagag tccttcgcga accgcatctc cccatcaatg
      841 tttttttgca gagcatggcc agagagtacg gagcttcttg cgtcgccgtc atcctttccg
      901 gtacggggag cgacggcgcg cagggaatca aggcagtcca cgcggcgggc ggcgcggtgt
      961 ttgcccagga cgtcgacagc tcccgtttcg acggaatgcc gcgcaacgcg gccctgacct
     1021 gcgtcgttga ttttgtcggt tccccggagc agatcgtctc cgctctcttc aaaaccgccg
     1081 gggacgaaac cggcgaaccg gataccaacg aagagcgcga gcgtctttgc gagaaggacg
     1141 cccttgaacc gaagcagctg atcttccgaa agttgagcga cgctttcggt atcgacttct
     1201 cccagtacaa ggacggcaca attctccgcc gtctggaccg gcggatgatg atgcgcgccg
     1261 tcgctgatat gtccgagtat gcggagctgc tcgaaggaga ttctgaagaa gtggatcatc
     1321 tctacaggga tttgcttatc gacgtcacgt cgttcttcag aaatccggag gccttctcct
     1381 atcttgccga gacagcgctt cccgatctgc tggcgcacca cccggaaaac gaggagtttc
     1441 gcgtctggag cgtagcgtgc gccagcggcg aggaagccta ctccctcgct atgattctcg
     1501 acgaggtgat ggcgaaccgc tcgcagaaac ggagcgtccg tattttcgcg acggatattc
     1561 acaagagttc cctgaagacg gcgagcgcgg ggtattattc gaacgaaaag ctcgccaacg
     1621 tttcgacggc gcggcgtgaa aaatatttct acaggacggg cgccgacggg tggacggtgc
     1681 gcccgaatat caggcggatg attacctttg cgccgcacaa tatcctctcc gatccgcctt
     1741 ttttgcacat gcaccttatt tcctgccgca acatgctcat ctaccttgag aaaagcgccc
     1801 agatcgaaac gctgacccgc ttttcggctg ctttggagca gcaaggcatt ctcttcctcg
     1861 gctccagcga gagcgtcctc gacacgcaga gcgagttttt gacagtggac agccgtctga
     1921 agatctaccg gaagcagggg acgattactt cccggccgct ctactccctc cttccggaaa
     1981 agaaaaaggc gactgccggt tccgaacggg gaagttcgaa aatgccgctt tcttcgctgt
     2041 atccgcttct tcttgagcgc tgcatgccga cgggctttct cgtgaactcg aagggcgatt
     2101 tgctccacac tttcggcggg gcgggaaaat atctgcagat catcggccct gtgaggacgg
     2161 atatcggcag cattttgcac gggaacctgc gcgtcgccgt catgacggcg atcgagcgtt
     2221 cccggaaaaa caggaccccc gtccgctttt cggatgtttc ccacccgctt ccaggcgctc
     2281 cgctgaacac tgtggaggtc tccgtggagc cgctcgaaga gcggacggac agccggtctg
     2341 aggagggcaa tctcttcttt ttcattcgcc tggaggagat gaagccctcg cttgccgcga
     2401 aggcggagtc attctccgtc gacgaagccg cgaaggcgcg catcgaagag ctggaacgct
     2461 cgctctcgga ggccagagag aaccttcaga cgatcgtcga ggagcttgag acgagcaacg
     2521 aagagcttca ggcgaccaac gaggagctgc ttgcatccaa cgaggagctc cagagcagta
     2581 acgaagagct gcagtcggtc aacgaggaga tgcacagcct gaacgcggag taccaggaaa
     2641 agaacgagca gttgatcgac ctcaacaacg atctgcagaa tctgatgagc tgcaccgaca
     2701 tcggcaccat cttccttgac ggagaattgc ggatacggcg ttttactccg gcgatcaaca
     2761 gagtattctt cctccgtgat caggatatcg gacgccccat agaggagatg cgcagccttg
     2821 tggagagcgg acacgacgtt gtcgcgtcga tcaggcgcgt tctcagggaa ggaaaagtcg
     2881 aggagatgga ggtgacgaac agagaaggcg tcccgatgct ccagcgcatt cacccgtacg
     2941 tggatatccg cggaacggtg agcggcgccg tgctgacgtt cgt
//
LOCUS       DAOPKU010000377         2980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37507_length_2980_cov_5.063248, whole genome shotgun sequence.
ACCESSION   DAOPKU010000377 DAOPKU010000000
VERSION     DAOPKU010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37507_length_2980_cov_5.063248"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2977)
                     /locus_tag="PK849_10945"
     CDS             complement(<1..2977)
                     /locus_tag="PK849_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPE66683.1"
                     /translation="MILAVLLGLSGMQGMGISLLPRIEVPFVSVVTRYTGAGPEDMET
                     LVTKPLEDAISQVQGARRIESTSLEGLSYVFVEFDMEVNLSDAALDVANRVRTVTLPE
                     DAEEPVVRKFDINARSFMSVVFTSTLPPQRAFDILEDRVQRQLSQVVDVADVSLDGGL
                     TREIHVDLDPVALGSLGLSIRQVTNLLKSGNYSSPSGRISLGDQESILRVVGESTTVR
                     ELEELQIPLAKGVSVRLADIATVTDATAEVRRLARYNGRDSFIISLTAVPDGNVVQAS
                     RAARAMLDEILPTLPPGFSMAIPFDDGEFIADSVWNVFRDMITGTLLTGLILFLFLRR
                     LSVTLLVGIVMPTAIIATFMPMVLARYTLNMMTTLGLAISTGILVNNSILVIENIIRY
                     RELGYNPLDAAERGTKEIAIAVLSTTATNLGVFIPMALSGGRMGQMFNPFAMTVVFST
                     LFSLWVALTFTPMLAARIGRSAGPSALNRFLTGWWQWLYQGFEELHHVLVRGAVRFPL
                     STILLFCALSAGSLMLAGRIGFQFFPSSDEGQITISMETSASASLSMTDRFVRQMEQH
                     AISLPYVSGVNAVIGGGGSRSGLNRATMRIYLEDTPDRPSSFAFAAPLRPLLASLPDM
                     KASVTAGGGRGGVRGKPIQVVVRGEEIGALNDLAVKVMEVLRGINGVVDVDLDWRTGR
                     PEIQMIPDRLRMARLNFNSEDLSDMLRGYVTGLKAGVFREGGKEYDILVKLRAEEVSS
                     PFQLPDLPFPTTAGFVSLRDLAALKETMGPTQILRKDRQRSITVDANVSGVTVGDVYR
                     VIEQRLGEIPLPQGYRFTFAGEIQSIQENFGTLNFALGLAVLLTFLLIAAILESFLFA
                     FVIMLTVPLSVIGVVPALILTQTPLSIYGLMGVIMLVGLVVNNAIVIVDYAEILRKEG
                     KEPSEAIIEACKVRLRPIIMADATSVIAMVPLAMGLGAGGSYRSPMAIVAIGGLIAGG
                     VLALFAIPPVYKLVWRVKLR"
BASE COUNT          745 a          866 c          825 g          544 t
ORIGIN      
        1 gtctgagctt caccctccag accagtttat agactggagg aatggcgaaa agagcgagga
       61 cgccgccggc gatcagacct ccgatggcga cgatggccat gggagaacgg taggatccgc
      121 cggcgccgag tcccatagca agaggaacca tggctataac cgatgtggcg tccgccatga
      181 tgatcggacg aagacggacc ttgcacgcct caatgatggc ctcggacggc tccttcccct
      241 ccttgcggag gatttccgcg taatccacga tcacgatggc gttgttgacc accaaaccga
      301 caagcatgat gacacccatc aaaccgtaga tggagagcgg cgtctgcgtc agaataagcg
      361 ccggtacaac gccgataacg gagagcggaa cagtgagcat aattacgaag gcgaagagaa
      421 acgactctaa gatcgccgca atcaaaagga aagtaaggag taccgcgagc ccgagagcga
      481 agttgagggt accaaagttc tcttgaatcg actgaatttc tcccgcgaag gtgaaacggt
      541 acccctgtgg aagcggtatc tccccgaggc gctgctcgat gaccctgtat acatcgccga
      601 cggtgactcc cgagacgttc gcatccaccg ttattgaacg ctgtctgtcc ttccgcagga
      661 tttgcgtcgg gcccatcgtc tccttcaacg cggcaagatc acggagcgag acgaagcctg
      721 ctgtcgtagg aaagggaagg tctggaagct ggaacggact cgaaacctcc tccgcgcgaa
      781 gcttgacgag gatatcgtac tccttgccac cctctcggaa gaccccggcc ttcagtcctg
      841 tgacgtatcc ccggagcata tcggagagat cctccgaatt gaaattcagc ctcgccatcc
      901 ggagtctgtc gggaatcatc tgaatctccg ggcggcctgt ccgccagtcg agatccacat
      961 caaccacacc gttaatgccg cggagaacct ccatgacctt cacggcaagg tcgttcagag
     1021 cgcctatttc ctctccccgg acaacaactt gtataggctt tccgcgaacg ccgccgcgtc
     1081 cacctccggc agtgacgctc gccttcatat ccgggagaga tgccaaaaga ggacggagag
     1141 gagccgcaaa agcaaagctg ctaggcctgt ccggcgtatc ctcaagataa atgcgcattg
     1201 tggcacggtt cagtcctgat ctcgaaccgc cgccgccaat aacagcgttg acaccggaga
     1261 cataaggaag tgaaatggcg tgttgttcca tctgccggac aaaacgatcc gtcatcgaga
     1321 gagaagcgga ggccgaggtc tccatgctga tggtaatctg cccctcgtcg gaggaaggaa
     1381 aaaattggaa cccgatacgc cctgcgagca tgagagaacc ggcggaaaga gcgcaaaaaa
     1441 gcaggatggt ggagagcgga aaacgtacgg cgcctcgcac aagtacgtgg tgaagctctt
     1501 caaaaccttg atagagccac tgccaccatc ccgtcaaaaa acggttcaaa gccgacggcc
     1561 cggcagacct tccgatgcgg gccgccagca tcggcgtgaa cgtcaaggca acccagagcg
     1621 agaagagcgt cgaaaagacc accgtcatgg cgaacgggtt aaacatctgc cccatgcggc
     1681 cgccggaaag cgccatgggg atgaataccc ccagatttgt cgctgtggtg gaaagcaccg
     1741 cgatcgcgat ctccttcgta ccgcgctccg cggcgtccag cgggttgtat cccaattccc
     1801 ggtacctgat gatgttttcg ataaccagga tagagttgtt cacaagaatg ccagtcgaga
     1861 tggcgagccc gagggtcgtc atcatgttga gcgtatagcg cgcaagcacc atcggcatga
     1921 aggtcgcaat tatggcagtc ggcatgacaa ttcccacaag cagcgtcacc gaaaggcggc
     1981 gcaaaaagag aaagagaatc aaccccgtca gtagcgtacc ggtaatcata tcccggaaga
     2041 cgttccacac cgagtcggcg atgaattctc cgtcgtcgaa cggaatagcc attgagaaac
     2101 caggtgggag cgtcgggagg atctcgtcga gcatcgcccg agcggcgcgg ctcgcctgta
     2161 caacattgcc atcgggaaca gcagtaagcg agatgatgaa cgaatcccgt ccgttgtatc
     2221 gtgcaagacg gcggacctcc gcggtggcat cagtgaccgt cgcaatgtcc gcaagacgta
     2281 cggaaacacc ttttgctaaa ggaatctgca gctcttccaa ctctcgcacg gttgtcgatt
     2341 caccgactac gcgtagaatt gactcctgat caccaagcga aatccgcccc gacggagagc
     2401 tgtagttacc gcttttcaga aggttcgtca cctggcggat actcagaccg agactgccga
     2461 gcgccacagg gtcaaggtcg acatgaattt cccttgtcag tcctccgtcc agcgacacat
     2521 cggcaacgtc gacaacttgt gaaagctgtc gttgcaccct gtcttcaagg atatcgaacg
     2581 ctctctgcgg cggaagcgtc gacgtaaaaa caaccgacat gaacgagcgt gcattgatgt
     2641 cgaacttcct gacaaccggt tcttcagcat cctccggaag cgtgaccgtt ctgacgcggt
     2701 tcgcaacatc gagcgcggcg tcggaaagat tcacttccat atcgaactcc acgaagacat
     2761 aggaaagccc ttcaagcgaa gtgctttcta ttcttcgggc cccctgcacc tggctgatgg
     2821 cgtcctccag cggcttagta acgagcgtct ccatatcctc cgggccggcc ccggtgtacc
     2881 gagtgactac ggagacgaac ggcacttcga ttcgcggaag aagcgagatc cccattccct
     2941 gcatacccga aagtcccaga agcactgcga gaatcacgat
//
LOCUS       DAOPKU010000378         2979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37524_length_2979_cov_3.808482, whole genome shotgun sequence.
ACCESSION   DAOPKU010000378 DAOPKU010000000
VERSION     DAOPKU010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37524_length_2979_cov_3.808482"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PK849_10950"
     CDS             complement(<1..460)
                     /locus_tag="PK849_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HPE66684.1"
                     /translation="MSSLVAQSAALLLVLLFLSAFFSASETAITSSGRGKILALIERY
                     PYQKRFFEWLLKDVQRALTIVLISNNLVNIAASAVGTTLAIATIGQGGVLLAVPLMTA
                     LIVIFGEVFPKSVAIIQSDFVLVFSLPFLRLLDILLAPFVWLMIAAVRLLG"
     gene            complement(457..987)
                     /gene="ybeY"
                     /locus_tag="PK849_10955"
     CDS             complement(457..987)
                     /gene="ybeY"
                     /locus_tag="PK849_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPE66685.1"
                     /translation="MKLQLRLDSSEDADPASTAFSYLVKRAEPVFTRALCDLFPEGTL
                     PAKVEISLSFLSLGEMKAQNRKYRGLDEPTDVLSFPLWEEDGVFRPGRSLPTLPLGDI
                     LICPEYVRAAVSSEEEFQREMALMLAHGFLHLLGWDHDTDERRSAMEVRQELIRKELL
                     AFPEGAASGTAEEELR"
     gene            complement(962..2470)
                     /locus_tag="PK849_10960"
     CDS             complement(962..2470)
                     /locus_tag="PK849_10960"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPE66686.1"
                     /translation="MDDDLTRMLRKEMGAALQPVERSIVPGDVLVEKGQIITDQAARI
                     LEMQGYSRVSFPWKQILFALVVIPFWPLWLRIQTRNHGGGEEKSELPWVYLAFIVGVS
                     WLAEYVSALSHAQGMGSLFLAGCAYLTLPAHLALQLVLGGAILGGLVVTGFSTLHVLL
                     VALMGILSAFGGYFLLIGVHSRGHLWKQLFLLGVLQTLTGLSLRWAFNISFSAEILIP
                     LILGSALWSSLVIAALPLLEDTFDVLSPLRLMELSHPSNPLLKRLQMEAAGTYHHSLM
                     LGTLAEAVADKLGMNANLLKAGAYFHDIGKLRRPDFFVENQMGGENVHDELKPSLSAL
                     VIIAHVREGLELAHEYHLPRVIRSFIAEHHGTTALAYFLRKARNMGLTIPREQFCYPG
                     PKPQSRETGLMMLVDSVEAAVRAESRAGSESILDVRDTINRVIDSKITEGQLDDVDFT
                     LRDLSVIRETLLSALRTVYHTRKVKEIQEKESPQHTTEKQEDDVSEASVASR"
     gene            complement(2474..>2979)
                     /locus_tag="PK849_10965"
     CDS             complement(2474..>2979)
                     /locus_tag="PK849_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66687.1"
                     /translation="LLLLVAFALTVIFLKWFFEDRISGFLPGEASSRTYFALYPMKYV
                     DEEGTVALRSRVGDTVSGVLVRDGAAMDRLISKIEIMAEGDVSLLSLSPGLTELIKSY
                     PEEARKRFLQASARLSSAYLQSQGEKPGGFSWSPEILWKEIERLALPMEENNIVYQIL
                     DEILQPL"
BASE COUNT          677 a          876 c          847 g          579 t
ORIGIN      
        1 gaccgagcag ccggaccgcc gcgatcatga gccatacgaa gggggcgagg aggatatcca
       61 gcagcctcaa aaacgggagc gaaaaaacga gcacgaaatc cgattgtatt atagcgacac
      121 tcttcggaaa cacctcgccg aagatcacta tgagcgccgt catcagcgga actgccagca
      181 ggacgcctcc ctgacctatc gtggcaatcg ccagagttgt gccgaccgcg cttgcggcta
      241 tgttgacgag attgttcgaa atcaagacga tggtgagcgc acgctgcaca tccttgagga
      301 gccactcgaa aaaacgcttc tgatacggat accgttctat cagcgcaagg atttttcctc
      361 tgccagagga tgtaatcgct gtttcagagg cgctgaaaaa ggcagataga aagagcagta
      421 ccagcaaaag cgccgccgac tgcgctacaa gagaactcac cggagctcct cctccgcggt
      481 cccggaggcc gcaccttcgg gaaaggccag cagttctttc cgtatcagtt cctgccgtac
      541 ttccatagcg cttctccgct catcggtatc gtgatcccac ccgagcaggt ggaggaaacc
      601 gtgagcaagc atcagcgcca tttctctctg gaattcctct tccgaagaga cggcggcgcg
      661 aacgtactcc gggcatatca gaatatcgcc caaaggaagc gtcgggagcg accgccccgg
      721 acggaagact ccatcctctt cccaaagggg gaaggagagg acgtccgtcg gttcgtccag
      781 tccgcggtat ttccggtttt gtgccttcat ttcgccgaga gagagaaagg acagggatat
      841 ctcgaccttc gcggggagag tcccctccgg aaaaagatcg caaagggcgc gcgtaaatac
      901 cggctccgcc ctttttacga gatagctgaa cgcggtactt gcgggatcgg cgtcctcgct
      961 gctatcgaga cgcaactgaa gcttcactga catcgtcctc ctgtttttct gtggtgtgtt
     1021 gaggagactc tttctcctgt atctctttta ctttgcgggt atgatacacc gtccgcagcg
     1081 ctgagagcaa tgtctcccgg atcaccgaga gatccctcag ggtaaaatca acgtcgtcga
     1141 gctgtccttc ggtgattttc gagtcgatca cccggttgat cgtatcccgg acgtcgagta
     1201 tcgactcgga acctgctctg gattccgccc ggacagccgc ttccaccgaa tcgaccagca
     1261 tcatcagccc ggtctccctg ctctgcggtt tcggacccgg gtaacaaaac tgctcccggg
     1321 gaatagtgag tcccatgttt ctcgccttcc gaaggaagta cgcaagcgcg gtggttccgt
     1381 ggtgctccgc gatgaacgac cggataaccc tgggcaggtg gtattcatga gcaagctcca
     1441 gcccttcgcg gacgtgcgcg atgataacca gcgccgagag cgagggtttg agctcgtcat
     1501 ggacgttctc cccgcccatt tggttttcga cgaagaagtc cgggcgccgc agctttccaa
     1561 tgtcgtggaa atacgcgccc gctttcagca ggttcgcgtt catcccgagc ttgtcggcca
     1621 ccgcctccgc cagcgtcccg agcataaggg agtgatggta cgttcccgcg gcctccatct
     1681 gcaacctctt gagcagcgga ttcgacggat ggctcagctc catcaggcga agcggcgaaa
     1741 gcacgtcgaa cgtatcttca agaagaggga gcgccgcgat gacgaggctg ctccagaggg
     1801 cgctcccgag tatgagcggt atcaatatct ccgcggaaaa ggagatgttg aacgcccatc
     1861 tgagcgagag tcccgtaagc gtctgaagga cgccgagaag aaagagctgc ttccagaggt
     1921 gccctctgga gtggacgcct ataaggagaa aataccctcc gaatgcgctg agtattccca
     1981 tgagcgctac aagcagcaca tggagcgtag aaaacccggt gaccacaaga ccgccgagga
     2041 ttgctccccc aagaacaagc tgaagcgcaa ggtgcgcagg aagcgtcaaa tacgcgcatc
     2101 ctgcaaggaa gaggctcccc atcccctggg cgtgagaaag ggccgagacg tactccgcaa
     2161 gccagctgac gccgacgata aaggcgaggt agacccacgg tagttcgctt ttttcttcgc
     2221 cgccgccgtg attccgcgtc tggatgcgga gccaaagagg ccagaagggg atgaccacga
     2281 gcgcgaagag tatctgtttc cacggaaaag acaccctgga gtagccctgc atttcgagga
     2341 ttctggcggc ctgatcggtg atgatctgcc ccttttcaac gagcacgtcg cccggaacga
     2401 tggaacgttc cacaggctgc agcgccgctc ccatctcttt ccgcagcatc cgggtgaggt
     2461 cgtcgtccac cctctagagg ggctggagaa tctcgtcgag gatctgatac acgatgttgt
     2521 tctcctccat tggaagagcc agcctctcga tctccttcca gaggatttcg ggagaccagg
     2581 aaaaaccgcc cggcttttcc ccctgggatt gaaggtaggc gctgctcagc cgggcgcttg
     2641 cctgcaagaa cctcttccgc gcctcttccg ggtaactttt tatcaactcg gttaatccgg
     2701 gagagagcga gaggagcgaa acgtccccct ccgccattat ctctatcttc gagataagac
     2761 ggtccatcgc ggcgccgtcg cggacaagga ctccggagac ggtatcgccg accctggagc
     2821 ggagcgcaac cgtcccttcc tcgtccacgt acttcatcgg gtacagggca aagtaggtcc
     2881 gcgaagaggc ctcccccggc aggaacccag atatccggtc ttcaaagaac catttcaaaa
     2941 aaataacggt cagcgcgaag gcaacaagaa ggagcaggg
//
LOCUS       DAOPKU010000379         2968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37729_length_2968_cov_3.900790, whole genome shotgun sequence.
ACCESSION   DAOPKU010000379 DAOPKU010000000
VERSION     DAOPKU010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37729_length_2968_cov_3.900790"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PK849_10970"
     CDS             <1..936
                     /locus_tag="PK849_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPE66688.1"
                     /translation="DAGDNLPYISTYCPAVLRLIQINFPELVGRILPVESRLETAVSI
                     WRDETGAEESVTLVSPCPAKATLVRNPVGREQSSLEYVVSVRKVIRDLLACNVKVTGT
                     KTKAISRRWLLWSISGGESRHISSFFDKGFTSISVSGLRNTQDLLNELELGRLAGVDF
                     IECRACDLGCIGGTGTYESRFLSQLRLDNIEAEWMPSGEEMEQIRSWYDKGIWRLAAP
                     VTAKERLPLSKDIKEAMAKLREMDAIYAELPHIDCGACGRPSCRALAEDIVRGQGEVT
                     DCVFKLRERITNLSGEIKNLSSKLPHTLHPGRKRR"
     gene            942..1271
                     /locus_tag="PK849_10975"
     CDS             942..1271
                     /locus_tag="PK849_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HPE66689.1"
                     /translation="MKIRDVIEKTGATVLAPGDAERELAGAISGDLLSFIMAEAKEGW
                     LWITIQVHLNVAAVAVLKDVPFILVASGRAPEQALVERCAAEGISLAVTKLPAFEAAG
                     ALRELAR"
     gene            1274..2032
                     /locus_tag="PK849_10980"
     CDS             1274..2032
                     /locus_tag="PK849_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPE66690.1"
                     /translation="MLSHFWVDLHLHTVLSPCGELEMGAPEIVAAYREAGIALCAVTD
                     HNDIANVPAIRGAASGNPVVLFGMEVQTAEDIHVVVLFPDDRTAEGYREWLWREMPQR
                     ANDPDIFGDQLVIDSENAILDEQQILLVQGVGYSVDEVASEAYERGALVIPAHIDRPS
                     FSYESVLGRIPDEFPCHALELSPAVSHAAFSEWKARYPERVFIRSSDSHRLSTIARDR
                     CTVMLLEAPSFEEIAMALNGQEGRSVLCPWVGAP"
     gene            complement(1987..2640)
                     /gene="hisIE"
                     /locus_tag="PK849_10985"
     CDS             complement(1987..2640)
                     /gene="hisIE"
                     /locus_tag="PK849_10985"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HPE66691.1"
                     /translation="MNIELNSVKFDEQGLIPVIVQDVLSGEVLMMAWANRESLELTAS
                     TGDMTFWSRSRKELWRKGATSGNTMKVLELRADCDGDALLALVEPSGPACHTGERSCF
                     FNVLTAPCEGRGTFPGVLWRYLEQRRYDDPAESYTARLLARGVRRVAQKVGEEGVETA
                     LAVAAGDKKETICEAADLLYHLQVALLAAGLSPRDVYAELERRHGAPTQGQSTDLPS"
     gene            complement(2630..>2968)
                     /locus_tag="PK849_10990"
     CDS             complement(2630..>2968)
                     /locus_tag="PK849_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HPE66692.1"
                     /translation="ATGLDVLEWIRDGARLGCGEILLTSMDRDGAKNGYDNRLLSRAA
                     SSVSVPLIASGGAGNMEHILETFRAGSDAALAASIFHYGEIRIGDLKRYLAGQGIPVR
                     LTGSVASNEH"
BASE COUNT          539 a          914 c          947 g          568 t
ORIGIN      
        1 gatgcgggcg acaacctccc gtatatttcc acctactgtc cggccgtcct tcgcctgatc
       61 cagatcaact tcccggagct cgtcgggcgt attctgcccg tggaatcccg gctggagacg
      121 gcggtttcca tttggcgaga tgagacggga gcggaggaga gcgtgacatt ggtctccccc
      181 tgtccggcga aagcgacgct cgtgcgcaat ccggtcggga gggagcagag ttccctcgaa
      241 tacgtggtga gcgtacggaa ggtgatacgg gacctcctgg cctgcaacgt gaaggtgacg
      301 ggcacgaaga cgaaggcgat cagcagaaga tggcttcttt ggtcgatctc cggcggcgag
      361 tcgcgtcaca tctcctcttt cttcgacaag gggttcacgt cgatctccgt ctcgggcttg
      421 cggaacacgc aggacctcct caacgaactt gagctcggcc gccttgcggg cgtcgacttc
      481 atcgagtgcc gcgcgtgcga cctcggatgt atcggcggca cggggacgta cgagtcccgt
      541 tttctctccc agctccgcct tgacaatatc gaagccgaat ggatgccctc gggggaggag
      601 atggagcaga tccggtcgtg gtatgacaag ggcatctggc gtcttgccgc gccggtgacc
      661 gcaaaagagc gcctgccgct ctccaaggat atcaaagagg cgatggcgaa gctgcgcgag
      721 atggacgcca tctacgcgga gcttccccat atagactgcg gagcgtgcgg ccgcccgtcg
      781 tgcagggcgc ttgcggagga tattgtccgg ggacagggag aagtaaccga ctgcgttttc
      841 aagctccgtg agaggataac caatttgagc ggcgaaatca agaatctctc ctcgaaactg
      901 ccccatacgc tccaccccgg aagaaaacgg aggtgacatt gatgaaaata cgggatgtca
      961 ttgaaaagac cggggcgacg gttctcgctc cgggagatgc tgaacgcgaa cttgccggag
     1021 cgatctccgg cgacctcctg agtttcatca tggctgaagc aaaggagggg tggctctgga
     1081 tcacgatcca ggtgcacctc aacgtcgctg cggtggctgt gctgaaggat gtgcccttta
     1141 tcctcgtcgc ttcgggaagg gcgccggagc aagcgctggt cgaacggtgc gccgccgagg
     1201 gtatttccct cgccgtgacg aagctccccg cctttgaagc cgcaggggcg ctccgggagc
     1261 ttgcgcgctg agcatgctct cgcacttttg ggtagacctg cacctgcata ccgtcctctc
     1321 gccgtgcggc gagctggaga tgggagcccc ggagatcgtc gctgcctaca gggaagcagg
     1381 tatcgctctc tgcgcggtga ccgaccataa cgacatagcg aacgttcccg caatcagggg
     1441 cgcggcgtct ggaaaccctg tcgtcctctt tgggatggag gtgcagacgg cggaggatat
     1501 tcacgtcgtg gtgctcttcc ccgacgaccg gactgcggaa gggtaccggg aatggctctg
     1561 gcgggagatg ccccagcgtg cgaacgatcc ggacatcttc ggcgaccagc tcgtgatcga
     1621 cagtgaaaac gcgattctgg acgagcagca gatactgctc gtccagggag tcggctattc
     1681 ggtcgacgag gtcgcgtccg aggcgtacga acggggcgcg cttgttatcc cggcccatat
     1741 cgacaggccg tcgttctcgt acgagtcggt gctcgggagg attcccgacg agttcccatg
     1801 ccacgccctc gaactctccc cggcggtgtc ccatgccgca ttttcagagt ggaaagcgcg
     1861 gtacccggag agagtcttca tccgctcttc cgattcccat cgccttagta ctatagcgag
     1921 ggatcggtgc accgtgatgt tgctcgaagc gccctctttc gaggagattg cgatggcgct
     1981 gaatggtcag gaggggagat ccgtgctctg cccctgggtc ggggcgccgt gacgccgttc
     2041 aagctccgcg tagacatccc tcgggcttaa acccgccgcc agcagcgcga cctggaggtg
     2101 gtagagcaag tcggcggctt cgcagatcgt ttccttcttg tcccctgcgg cgaccgcaag
     2161 cgccgtttcg acgccctcct cgccgacctt ctgggcgacc cggcgaacgc ctcgcgcaag
     2221 caacctcgct gtgtaactct ccgctgggtc gtcgtagcgg cgctgctcca ggtagcgcca
     2281 gagaacgccg ggaaacgtcc ctctcccctc gcacggtgcg gtgagcacgt tgaaaaaaca
     2341 ggaacgctcc cccgtatggc aggcggggcc cgacggctcc acgagcgcga gcagcgcgtc
     2401 gccgtcgcag tcggcccgca gttcgagcac cttcatcgtg ttgccgcttg tcgctccctt
     2461 cctccacagc tccttccgcg atctgctcca gaaggtcatg tcgcccgtcg aggcggtcag
     2521 ttccagagac tcgcggttcg cccacgccat catgaggacc tcccccgaga ggacgtcctg
     2581 gacgatgacg gggatcagcc cctgttcgtc gaattttacg gaattcagct caatgttcat
     2641 ttgaagcaac actcccggtg agccggacgg ggattccctg cccggcaaga tatcgtttga
     2701 gatcgccgat gcgaatctcc ccgtagtgga agatggaggc ggcgagcgcg gcgtcgctcc
     2761 ctgcgcggaa ggtctccagg atgtgctcca tgttcccggc cccgcccgag gcgatcagcg
     2821 gaacggagac ggaggaggcc gccctggaga gcagacggtt gtcgtagccg ttcttcgccc
     2881 cgtctcggtc catcgaggtc agcagaatct cgccgcaccc gaggcgcgcg ccatccctga
     2941 tccattcgag tacgtccagt ccggttgc
//
LOCUS       DAOPKU010000380         2958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37886_length_2958_cov_4.787117, whole genome shotgun sequence.
ACCESSION   DAOPKU010000380 DAOPKU010000000
VERSION     DAOPKU010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37886_length_2958_cov_4.787117"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /gene="pgsW"
                     /locus_tag="PK849_10995"
     CDS             complement(<1..613)
                     /gene="pgsW"
                     /locus_tag="PK849_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HPE66693.1"
                     /translation="MWRKVRAAQTSLAAWRDASQLPALYPEYDFSKTDPWRSGFIGIE
                     WSPVTTTLGPIEAKRTAADPLWSVYALREFRKFGLKEGETIAMISSSSFPGLVYSLLA
                     AAEHLGLRVLWIHSLGASTWGANIPEMSWPKIGELLRNGGFLSRKPDWYTLGGGNESG
                     GGMSPEGRKALLAAALHDGVPMLEAATLQEMIELKTKHILSVKP"
     gene            complement(742..1179)
                     /locus_tag="PK849_11000"
     CDS             complement(742..1179)
                     /locus_tag="PK849_11000"
                     /inference="COORDINATES: protein motif:HMM:NF025467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HPE66694.1"
                     /translation="MTEHSLLLVGLGICLGLFFFHRTGYSPGGIITPGFLALELGSPE
                     RVAAAFVIGGCVAALLSLVVRVTGAYGRQRTGIALLLALAFRLFAGGGTTLSYLWIGW
                     VVPGLIGADMQRQGAIPTIGAALSTAFASAMAARLLISAGALL"
     gene            complement(1176..2231)
                     /locus_tag="PK849_11005"
     CDS             complement(1176..2231)
                     /locus_tag="PK849_11005"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPE66695.1"
                     /translation="MNSIHSIPIRILVTGSRGKSSLVRLLSAALVSFGLNVRGRITGV
                     LPRELLPGDGILSPLKETLLLRSGPASVEEMRWWLSTLPRGTDAVVMENSAVAPELQA
                     LAFRWLQPCCTVLTNVRPDHEEVWGRGEENAASALCSGIGDPGGMTCPEHPVVLPRSV
                     AEKQCVQRLLSAQQCRLLPCGEGNAFKETHLALVQEVCRLFGFDERQSRAAAAALPPD
                     VADFAFFREGDGVLAAAFSANDTESTEQLFVSTGWSREETTLLFNARKDRVRRFRSFL
                     PWIRSFPWKKVCVTGGRPLFLSSPAVYSPLLDAPSLAGFIRSTGRVFGCGNVAGTPLE
                     YLESKFLQFRERQDGVS"
     gene            complement(2325..>2958)
                     /gene="deoC"
                     /locus_tag="PK849_11010"
     CDS             complement(2325..>2958)
                     /gene="deoC"
                     /locus_tag="PK849_11010"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPE66696.1"
                     /translation="AKYIDHTLLKPDASEEAVLKLCAEAREFGFASVCVNGSFTELVA
                     RELKGSGVKTCVVVGFPLGAGTSAAKAFETADAVSRGAEEIDMVISVGRVRSGETASV
                     REDIRAVVQAAEGALVKVIIETCLLTDEQKKLACCLAEEAGAHFVKTSTGFSTGGATV
                     EDVALMRRVVGDRLGVKASGGIRTREDAERMIAAGASRIGASASVGICAR"
BASE COUNT          685 a          919 c          836 g          518 t
ORIGIN      
        1 gcggtttcac ggaaagaata tgcttggttt tcagctcgat catctcctga agcgttgcgg
       61 cttcgagcat cggaacaccg tcgtgcagcg ctgcggcaag aagcgccttt cttccttccg
      121 gcgacatacc gccgccggat tcgttccctc cgcccaaagt ataccaatca ggctttcgtg
      181 agaggaagcc tccatttcta aggagttctc ctatcttcgg ccaggacatt tccggaatgt
      241 tcgcgcccca agtggaggcg ccaagcgagt ggatccacag gacgcgaaga ccaagatgct
      301 cggcagcggc aagaagcgaa tacaccaggc cggggaaaga ggacgaagag atcatggcga
      361 tagtctctcc ttctttgagc ccaaacttcc ggaattcccg caaagcgtag acgctccaga
      421 gaggatccgc agctgtccgc ttcgcttcta tcggtccaag agtcgtcgtc accgggctcc
      481 attcgatacc gatgaaaccg cttctccacg gatctgtttt gctgaagtca tattccgggt
      541 acagggcagg gagctgcgac gcatcgcgcc acgcagcaag gctcgtctgg gcggcgcgca
      601 ccttccgcca caaagcctct tcctcctccg aaagaaacga aacgccttga aaaaaccaga
      661 gaaaggcgag cacgcacgcc ggtatgagta atccccatcg tgcccgacga gcaaaggcgc
      721 gggaccgcgc gctcaatccg ttcataagag cgctccggcg gaaatcagca aacgggcagc
      781 catcgcggag gcgaacgccg tggaaagcgc agcgccgatg gtcggaatcg ctccctgccg
      841 ttgcatatcg gcgcctatca gcccgggaac gacccatcct atccacagat acgagagcgt
      901 cgtccccccc ccggcgaaca atctgaaggc aagcgcaagc agcagggcga tccccgttcg
      961 ctgacggcca tacgctccgg ttacacgtac gacgagcgag aggagggccg caacacatcc
     1021 tccgatgaca aatgctgcag cgactctttc cggcgagccg agttcaaggg cgagaaaacc
     1081 gggcgtaatg atgccccccg gggagtaccc tgtgcggtgg aaaaagaaca agcccaggca
     1141 gatccccagt ccgaccagaa gcagactgtg ttccgtcacg atacgccatc ctgcctttca
     1201 cgaaattgca gaaatttcga ctccagatac tcaaggggag tccccgccac gttgccgcac
     1261 ccgaagaccc tgcccgttga acggataaaa cccgcaaggg acggggcgtc aaggagcggc
     1321 gaatacacgg caggggatga taaaaagaga ggtcggccgc cggtaacgca gactttcttc
     1381 cacgggaagg aacgaatcca tggaagaaat gacctgaaac gcctcaccct atcctttctt
     1441 gcattgaaaa gaagagtggt ctcctctctg ctccatccgg tggatacaaa aagctgctca
     1501 gttgattcgg tgtcgtttgc agaaaaagct gccgcaagca ccccgtctcc ctcgcggaaa
     1561 aaggcgaaat cagccacatc cggaggcagc gcggcggcgg cggcacggct ttgccgctcg
     1621 tcgaagccga agagacggca gacctcttgg acaagggcga ggtgcgtttc tttaaaagcg
     1681 tttccttcgc cgcagggaag aagacggcac tgttgtgctg agagcaggcg ctgcacacac
     1741 tgtttttcgg cgacagaacg gggaagaacg acaggatgct ccggacaggt catacccccg
     1801 ggatcgccta ttcctgaaca gagcgcagac gcggcgttct cttcgcctct tccccaaacc
     1861 tcctcgtggt cgggacgaac atttgtgagc acggtgcagc acggttgaag ccaccggaag
     1921 gcaagggcct gcaattccgg ggcgaccgcg ctgttctcca tcacgacggc gtccgttccc
     1981 ctgggaaggg ttgaaagcca ccagcgcatc tcctcgacgc tcgccgggcc ggaacgaagc
     2041 agaagagtct ccttcagagg agataagatc ccgtcgccgg gcagaagttc tcttgggaga
     2101 acccctgtga tccgtccgcg gacgttgaga ccgaaggaga ccaaagcagc cgaaagaagg
     2161 cggacgaggg agctttttcc ccggctcccg gtaacgagta ttcggatggg aatcgaatga
     2221 atgctgttca tgaagtatat tgtacacctc gcgccgaaag aagaaaaaga aaacggggcc
     2281 cccgaaagcg gcggaaccgc tccgggaacc ccgtgcggaa ttcgttatcg ggcgcagata
     2341 ccgacggaag cggaggcccc gatacgcgaa gctccggcgg cgatcatacg ttcggcgtct
     2401 tctctcgtgc ggataccgcc cgacgccttt acgccaagcc tgtcgcccac gacgcgccgc
     2461 atcagcgcga catcctcgac cgtcgcgcct cccgttgaaa aacccgtcga ggtcttcacg
     2521 aagtgcgctc ccgcctcctc ggcaagacaa caggccagct tcttttgctc gtccgttaac
     2581 agacaggttt ctatgatcac cttcacaagt gcgccttcgg cggcctgtac cacggcgcgg
     2641 atgtcctcac gtacggaggc cgtttccccg gaacgcactc gtcctacaga gatcaccata
     2701 tcgatctcct cggctccgcg agaaaccgca tcggctgttt caaaagcctt cgccgcagac
     2761 gtcccagcgc caagcggaaa cccgacgacg acgcaggtct tcacaccact tcccttcagt
     2821 tcgcgggcga ccagttctgt aaaactgccg ttgacgcaca ccgaagcgaa tccgaattca
     2881 cgcgcctcag cgcagagctt gaggaccgcc tcttccgagg catccggttt caggagcgta
     2941 tggtcgatgt acttcgca
//
LOCUS       DAOPKU010000381         2957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_37906_length_2957_cov_4.321158, whole genome shotgun sequence.
ACCESSION   DAOPKU010000381 DAOPKU010000000
VERSION     DAOPKU010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37906_length_2957_cov_4.321158"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK849_11015"
     CDS             <1..452
                     /locus_tag="PK849_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPE66697.1"
                     /translation="DAMNDTKIGVSGGRFLAFRLAPGEELLGALRRIVAANGIRAGYV
                     AGVVGSLSRACLRFAGRRDGAALDGCFEVVSLVGTLDAEGEHLHMTISNQEGTTTGGH
                     VLEGCVVRTTMELVLGDLSDLEFRREYCPLSTYGELAVIPRKNNKGE"
     gene            456..1019
                     /locus_tag="PK849_11020"
     CDS             456..1019
                     /locus_tag="PK849_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPE66698.1"
                     /translation="MKNRTLVLVALCIALNMGLGQIASTLKLPIFLDSIGTVLSAILM
                     GPWIGMLTGLCTNLIWGVLTSPVAAAFAPVAMVIGLVAGVLARKGMFRSLPLVIVSGI
                     VVTIFVTLVATPIRTYLFAGVTGSGADFFVAYLTAVGQKLLQSVAITVIWTNLVDKIV
                     ACFVAWLLVKQLPERVKSLFPDASNVA"
     gene            1098..>2957
                     /locus_tag="PK849_11025"
     CDS             1098..>2957
                     /locus_tag="PK849_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE66699.1"
                     /translation="MLLPALPFVAVMAGLSAVALCVWPKSRSSVKGVALFMAPLAFGF
                     WLIHGGVFASLIGGVVQQTRGLWAFGLWLRILSVVAASRLWLASSPPPLLIRSLLASS
                     LPASFAFLLASPLLLTEQIKARIAQIQEAQLARGVPVHGSFRERASSLTALLFPLVLG
                     LLNDLPSRSAALDMKAFGLFRRRSSLFGGREPRVPRTPRPVSRDGHGETSRTGESEKA
                     LGGSGGGDVFREPAEIAENAESRCASASPDFAPIPEASRPPEAANSPGALPLLALDDA
                     RFTAPGAEAPIVTVPALRLFPGEWMLLEGGNGAGKSTLATMLCGGVPEHRSGILSGRA
                     ELSGEPVASRTSLQWSPDVQLVQQNPQLSLSGCAFTVYEEVAFGPENLALPLGEIRRR
                     VDDALELFGITHLKEHHPATLSGGEAQKAALASAAAMRPRLLLLDEAFSRIASADVPV
                     LLERLRSWSAKHSTAVLLLERRGQSFERFCDRFGTFLDGVVLERDEETVGRSAAGRSR
                     PEAVPSVVRGYTGSFCVGKRRKDEDRPSFGNVVLEQDDSGESACMDRFSKTSAAKEGA
                     ALLCEESPALLRILDLDFRWKGRSEPLLRSLNAELRAGERVALVGPNGAGKSTL"
BASE COUNT          429 a          943 c         1012 g          573 t
ORIGIN      
        1 aggacgcaat gaacgacacg aagatcggcg tctccggagg tcgctttttg gccttccggc
       61 tggctccggg ggaagagctt ctcggggcgc tgcggcggat cgtcgccgcg aacggaattc
      121 gggcggggta tgtcgccgga gtggtcggca gtttgtcgcg ggcgtgcctg cgctttgcgg
      181 ggcgtcgcga cggggcggcg ctcgacggct gcttcgaggt ggtttcgctc gtcgggacgc
      241 tggacgcgga gggcgaacac ctgcacatga cgatctcgaa ccaggaggga acgacgacgg
      301 gggggcacgt gctggaaggg tgcgtcgtcc ggacgacgat ggagctcgtg ctgggcgacc
      361 tctccgacct ggagtttcgc cgcgaatact gccctctttc cacatacggg gaacttgcgg
      421 tcataccgcg aaaaaacaat aaaggggaat gaacgatgaa gaacagaacg ctggtacttg
      481 tagctctttg catcgcgctg aacatggggt tggggcagat cgcctccacg ctgaaactgc
      541 cgatattttt ggattcgatc gggacggtgc tctccgccat cctgatgggg ccgtggatcg
      601 ggatgctcac cgggctgtgc accaacctga tctggggcgt gctgacgagt cccgtagcgg
      661 cggccttcgc ccccgtggcg atggtcattg gcctcgtcgc gggcgtgctg gcgcgcaagg
      721 ggatgttccg ctcgctgccg ctcgtgatcg tctccggcat cgtagtgaca atcttcgtaa
      781 ccctcgtggc cacgccgatc cgcacctacc tcttcgccgg agtcaccggt tccggcgccg
      841 acttcttcgt cgcctacctg accgccgtcg ggcagaagct gttgcagtcg gtggcgatca
      901 cggtcatctg gacgaatctt gtggacaaga tcgtggcgtg cttcgtcgcc tggctgctcg
      961 tgaaacagct tccggagcgc gtcaagtcgc tcttcccgga cgcttcgaac gtcgcctgag
     1021 gcgctgtgga gccactgtgc gcactttcgg ggccgttggg ctcctttgtc ttgtggggaa
     1081 tttgggtcgg gggctcgatg ctgcttccgg cccttccctt tgtggccgtc atggcgggcc
     1141 tttccgcagt cgcgctctgc gtctggccaa agtcgcgaag cagtgtgaaa ggggtcgcgc
     1201 tcttcatggc gccgctggcc ttcggctttt ggctgatcca cggcggcgtc ttcgcctcgc
     1261 tgatcggcgg cgttgtgcaa cagactcgcg gactgtgggc gttcgggctt tggctccgca
     1321 tcctctccgt cgtcgcggca agccgcctct ggctcgcctc ctctcctccc ccgttactca
     1381 tccgctcgct tttggcgagc agtcttccgg cgagcttcgc cttcctgctg gcgagcccgc
     1441 tgctgctgac cgagcagatc aaggcgcgga tcgcccagat tcaggaggcg cagctcgcgc
     1501 gcggcgtccc ggtgcacggt tctttccgcg aacgggcctc ctcgctgacg gcgctgctct
     1561 tcccgctggt gttggggctg ctgaacgacc tcccctcacg gagcgccgcg ctcgacatga
     1621 aggcgttcgg gctgttccgg cggcgttcga gccttttcgg aggcagggag ccgcgcgtcc
     1681 cgcgcactcc gaggccggta tcccgcgacg gacacggtga aacgtcccgg accggcgagt
     1741 cggagaaagc gctcggagga tcgggcggag gcgatgtgtt ccgcgaaccg gcggaaattg
     1801 cagaaaatgc ggagagccgc tgcgcctccg cttcgccgga tttcgcaccg atccccgaag
     1861 cctctcgtcc tccggaggct gcgaactctc ccggcgcgct tcccctcctc gcgctcgacg
     1921 acgcgcgttt caccgcgccc ggcgcggaag ccccgatcgt gacggttccc gcgctgcgcc
     1981 tcttccccgg cgaatggatg ctgctcgaag gcggaaacgg cgccggaaag tccacgctgg
     2041 cgacgatgct ttgcgggggc gttcccgagc acagaagcgg catcctttcg gggcgcgcgg
     2101 agctgtccgg cgaaccggtc gcctcccgga cgagtctcca gtggtcgccg gacgtgcagc
     2161 tcgtccagca gaaccctcag ctctcccttt cggggtgtgc cttcacggtg tacgaagagg
     2221 tcgccttcgg gccggagaac cttgcgctcc ccctcggcga gatccgccgc cgcgtcgacg
     2281 acgcgctgga gctgttcggg ataacgcatc tcaaggaaca tcaccctgcg actctctccg
     2341 gaggggaggc gcagaaggcc gccctggcct ccgcggcggc gatgcgcccc cgtctgctgc
     2401 tgctcgacga ggcgttcagc cggatcgcct ccgccgatgt gcccgtgctg ctggagcgac
     2461 tgaggtcgtg gtcggcaaag catagtactg cggtgcttct gctcgaacgg cgcggacagt
     2521 ccttcgagcg cttctgcgac cgcttcggta ctttcctgga cggcgtcgtg ctggaacgcg
     2581 atgaagaaac ggtcgggagg agcgccgccg ggcgttcgcg accggaagcg gttccgtcgg
     2641 ttgttcgcgg ctacacgggg agtttctgcg tcgggaagcg acgcaaagac gaagacaggc
     2701 catctttcgg aaacgtcgtt cttgagcagg acgattccgg agaaagcgcg tgcatggacc
     2761 gcttctcgaa gacgagcgcc gcgaaggagg gagctgcgct tctctgcgaa gagagtcccg
     2821 cccttcttcg gattctggac ctcgacttcc gctggaaggg ccggagcgag ccgctgctcc
     2881 gctcgttgaa cgccgaactt cgggcagggg agcgcgtcgc tcttgtgggg ccgaacggcg
     2941 cgggcaagtc cacgctg
//
LOCUS       DAOPKU010000382         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_38013_length_2951_cov_4.265884, whole genome shotgun sequence.
ACCESSION   DAOPKU010000382 DAOPKU010000000
VERSION     DAOPKU010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38013_length_2951_cov_4.265884"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK849_11030"
     CDS             <1..181
                     /locus_tag="PK849_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66700.1"
                     /translation="VPMAANLAALGAVVGLTGAARPESVRAVLEERAFRQGSAEAFDE
                     GLRAAKTGERIPPRN"
     gene            366..1469
                     /locus_tag="PK849_11035"
     CDS             366..1469
                     /locus_tag="PK849_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPE66701.1"
                     /translation="MQQQHAERVYFFDAYRTIIVLSVLALHAAMSYMAYVPGWWYVIN
                     PEKSLFFLFWVLIVNTGDMPAIFFLAGYFAPRSLEKRGRMLFLKEKALHIGIPWIVGV
                     LAFAPLFAMATWRSLNLPLPETYMEFVRTIWLGPGYQQSHFWFLGVLMVFLIVFAAVG
                     SPRAASGRSPLFWLAGWWGLSSAAFFLSSLYLHPDLWIRISHIYFQPARIVSYALAFY
                     LGARAWRDGWFDGPRPGFGAIALSGLATVAGSWSVIFLAMNFPVKETLALKALHGMSH
                     SFASLSIAVFFLFLCRALFDRPSPVWRTLSEASYGIYWLHQMILMPAVYLLIPWNLPI
                     GIKFILALGGTYILCAFLTIHGLKKLPVLRRLF"
     gene            complement(1673..2386)
                     /locus_tag="PK849_11040"
     CDS             complement(1673..2386)
                     /locus_tag="PK849_11040"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPE66702.1"
                     /translation="MKEIYYSITNDVFSPFPDYVRGVVVARGVKNGPSPAELTALLRE
                     AEEQLRGKLGSGNIAEHPRIASWREAFRKTGVKPAEFRSSIEAMARRVAKGQELPSIN
                     ALVDIGNVLSLRHLVPTGGHALDDVRGDIVLRAATGEETFVPFGETETEHPLPGEIIF
                     AEGDVVMTRRWSWRQGVHTLTLPESTSIEFNVDGLPPVTSGEVESVCGEVVELVSRFC
                     GGTFEKGLLTAASPRMRLL"
     gene            complement(2399..>2951)
                     /locus_tag="PK849_11045"
     CDS             complement(2399..>2951)
                     /locus_tag="PK849_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE66703.1"
                     /translation="NTVLERVLERNFAELGVPEYTEEEISFARAIFDGIAPSERSSDL
                     DQYSAPGGAAGKEAAKRLRERPIADEVLPYGESDFVLPGSTDVGDVSWIVPTCQAWVA
                     TWPNHTPGHSWQIVSSGVTPLAHKGMLHAGKVLAAAAADLFDDPSLIDAAKKELRERL
                     DGVSYRCAIPDEVVPKAIQPSGR"
BASE COUNT          460 a          998 c          929 g          564 t
ORIGIN      
        1 ggtgccgatg gcggccaacc ttgcggcgct gggcgccgtc gtcgggctga cgggcgcggc
       61 gcgtccggag tcggtacgcg ccgtcctcga agaaagggcg ttcaggcagg ggagcgcgga
      121 ggcattcgat gaagggctcc gcgctgcgaa aaccggagag cggattcctc cgaggaatta
      181 gcccggcggg gagccggatt ccgcgttttt tgtccgtcaa gcatctcccc ggaacggcag
      241 caaggacact gaatggaaac cggatgcgca cggacggaat cctttcgcgg cgcggttttt
      301 tccggcgtcc cgttgtgcag aacaatgagg atacagttca tcaaaaaaaa gcgaggtggg
      361 atttcatgca acagcaacac gcggagcggg tctacttctt cgacgcctac cggacgatca
      421 tcgtcctctc cgtcctggcg cttcacgcgg cgatgagcta catggcctat gttccggggt
      481 ggtggtacgt catcaacccc gagaagagcc tcttcttcct cttctgggtg ctcatcgtga
      541 acacgggcga catgcccgcg atcttcttcc tggcggggta cttcgccccc cgttcgctcg
      601 aaaaaagggg gcggatgctc tttctgaagg aaaaagcgct ccacatcggc attccatgga
      661 ttgtcggcgt cctcgcgttc gcgccgcttt tcgccatggc gacgtggcgc tcgctgaacc
      721 tgccgctgcc ggagacgtac atggagttcg tccggacgat atggctcggc cccggctacc
      781 agcagtcgca cttctggttc ctcggcgtgc tcatggtctt tctgatcgtc ttcgccgccg
      841 tcggctcccc gcgcgccgcc tccgggcgct cgcccctctt ctggcttgcg gggtggtggg
      901 gcctctcctc cgccgcgttc ttcctttcgt ccctctactt gcacccggat ctctggatac
      961 gaatcagcca catctacttc cagcccgccc ggatcgtctc ctacgccctc gccttctacc
     1021 tgggagcccg ggcgtggcgc gacggctggt tcgacggccc ccggcccggc ttcggcgcga
     1081 tcgcgctctc ggggctggcc acggtcgcgg ggtcgtggtc ggtgatcttc ctcgccatga
     1141 acttccccgt gaaagagacg cttgccctca aggcgctgca cggcatgagc cactccttcg
     1201 cctccctttc catcgcggtc ttcttcctct tcctctgccg ggcgctcttc gaccgtccgt
     1261 ctccggtatg gaggacgctg agcgaggcgt cgtacggcat ctactggctc caccagatga
     1321 tcctgatgcc cgccgtctac ctgctgattc cgtggaacct cccgatcggc atcaagttca
     1381 tcctcgccct cgggggcacg tacattctgt gcgcgttcct cacgatccac ggactgaaga
     1441 agcttccggt cctccggcgg ctcttctgac gctcccgtgc agggacaaag gacgcgcggc
     1501 tcccgagttt gcatgggggc cgcgcgtttg tgttttcgga gaatccgtaa ctgttatagc
     1561 ggccagaatc ataccgcatt atcccgctgc gaggacgcga gcgatcgaca gcgcaaaacc
     1621 tccggcacgc ttccgccgga caaacgagga aaactcccgt ttactcgcct gcttacagca
     1681 ggcgcatccg gggagaggcc gccgtgagca gtcccttctc gaacgtgccg ccgcagaagc
     1741 gcgacaccag ctcaacgacc tcgccgcaga cggactccac ctcgccggac gtcaccggcg
     1801 gcagtccgtc cacgttgaac tcgatggagg tcgactcggg cagcgtgagg gtgtgcaccc
     1861 cctgccgcca gctccaccgc cgggtcatga ccacatctcc ctccgcgaag ataatctcgc
     1921 cgggaagcgg gtgttcggtc tccgtctcgc cgaagggaac aaaggtctcc tctccagtgg
     1981 cggcgcggag gacgatgtca ccccgcacgt cgtccagcgc gtgcccgccc gtcgggacga
     2041 gatggcggag cgagaggacg ttgccgatat ccacgagggc gttgatcgac ggaagctcct
     2101 gccccttcgc gacgcggcgc gccatggcct cgatggagga acggaactcg gcgggcttca
     2161 cgcccgtctt ccggaaggcc tcccgccacg acgcgatgcg cgggtgctcc gcgatgttac
     2221 cggagccgag ctttccgcgc agctgctctt cggcctcgcg gagcagcgct gtaagctccg
     2281 ccggagaggg accgttcttc acgccgcgcg cgaccacgac ccctcggacg taatcgggga
     2341 acggggagaa gacgtcgttc gtaatgctgt agtaaatttc tttcatgcgt tgttcctcct
     2401 atctgcccga tggctggatc gccttcggga cgacctcgtc ggggatggcg cagcgatacg
     2461 aaacgccgtc gaggcgctcg cggagctcct tcttcgcagc gtcgatcagc gatgggtcgt
     2521 cgaacaggtc ggcggccgcc gccgcgagga ccttccccgc gtggagcatc cccttgtgcg
     2581 caagcggggt gacgcccgag gagacgatct gccacgagtg ccccggagtg tggttcggcc
     2641 acgtcgccac ccaggcctgg caggtgggaa cgatccagct cacgtcgccc acgtcggtcg
     2701 atccggggag gacgaagtcg ctctctccgt agggaagcac ctcgtcggcg atcgggcgct
     2761 cccggaggcg tttcgccgcc tccttgcccg ccgctccccc cggcgcggag tactggtcga
     2821 ggtcggagct gcgctcggag ggcgcgatcc cgtcgaagat cgcccgggcg aaggagatct
     2881 cctcctccgt gtactccggc acgccgagct cggcgaagtt ccgctcgagt acgcgttcga
     2941 gcacggtgtt c
//
LOCUS       DAOPKU010000383         2945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_38125_length_2945_cov_4.489619, whole genome shotgun sequence.
ACCESSION   DAOPKU010000383 DAOPKU010000000
VERSION     DAOPKU010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38125_length_2945_cov_4.489619"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..1537)
                     /locus_tag="PK849_11050"
     CDS             complement(353..1537)
                     /locus_tag="PK849_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPE66704.1"
                     /translation="MKPLEGLVVLDMTRVLAGPFAAMMFADMGAEVIKIERPNSGDDT
                     RAYPPFQAGESAYFMSLNRGKKSVTLNLKHEKGKEILKSLAKKSDILIENFKPGTMEK
                     LGLGYEDLRKVNPRLIYAASSGFGQTGPYSRRPAMDVIIQGMGGMMSVTGPDEHTPTK
                     VGSSIADIFAGLFTTIGILSAVNSRMKTGRGQLVDVAMLDCMVAVLENAVSRYLVTGE
                     IPRPMGNKHPAISPFATLATADGVMNIAVGNDDIWHRFCGVLGMEEFENDPRFADNPG
                     RVKNFDALKVLMEERTVRQTTEYWLSILEKNHVPCGPINDMAHVVNDPQVLARKMIVD
                     VLHPIAGATKIPGVPIKFSETPSEIEAPAPVLGEHTEKILTSYLGLSAAEFEELKKDG
                     VV"
     gene            1877..>2945
                     /locus_tag="PK849_11055"
     CDS             1877..>2945
                     /locus_tag="PK849_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HPE66705.1"
                     /translation="MIVADLLKKNLFPDFQILAGSGGVGREISAVSVLEAPDADRWMR
                     GGEFLVGSGFVFKDDPEQLTPLLRRMNEKGVAAIGFKLDRFHHKLPASMTAEADALQI
                     PILEIPMHYRWTDIIELIHTILVQERLKERPDDLTSFWEESLDYKKLMSGFAQNLGRD
                     LLVQAPQLELNNLFLADGSILGGEAVQKALDTPLVQEQPLPKKGQIHSCVEVRNSGTI
                     RRFAAYRLRLDTPISIFMILSPGELAPSARQERMLLRALTMLRASALEIAILSSKKVL
                     KKERFLEGLCFGMYCDPNMIRINLQELGIPLSLPVTVLIVSTADGLSTPRWSSPSQLS
                     YRLGNTWVTLIDESETAAWKKS"
BASE COUNT          620 a          882 c          829 g          614 t
ORIGIN      
        1 gaagcgacgg gtcgtaccgc gcagggattc cctcccccgg tagagagaga agctctccct
       61 gccgcagagg gggaagaagg tccgtcagcc acgtcctcgg gccgaaatcc tcccctgacg
      121 tgacaacgga ataccgccgt ccgctttcgg gttcttcaag gttcactgtt cgcgtatata
      181 tttcccgcac ccgcagcaag gtgaattcac cgccccgaaa ggggcgatga agagaaggtc
      241 ccctggggga cgccggaagg agaccctctt tccgattcgc agtccgggag agcacatcgc
      301 cgctgcaggc gcggagctgc atgctctttc aaccgccctg tattgccgga ggctacacca
      361 cgccgtcctt tttgagctcc tcaaactcgg cggcggagag accgagatac gacgtcagta
      421 tcttctcggt atgctcgccg agcaccggcg cgggggcctc gatttcagag ggcgtttccg
      481 agaacttgat cggaacgccg gggatcttcg ttgcgccggc tatcgggtgg agtacgtcga
      541 cgatcatctt tcgcgcaagc acctgcgggt cgttgacgac gtgcgccata tcgttgatcg
      601 gaccgcaggg aacgtgattt ttctcaagta tggaaagcca gtattccgtc gtctgacgta
      661 ctgtacgctc ctccatgagc actttcagcg catcgaaatt cttcacccgc cccgggttgt
      721 ccgcaaagcg gggatcattc tcgaactcct ccatcccgag gacgccgcag aatctgtgcc
      781 agatgtcgtc gttgcccaca gcgatgttca tcacgccgtc agcggttgca agggtggcga
      841 aaggcgagat cgcagggtgc ttgttgccca tcggacgggg aatctctccg gtcacgaggt
      901 agcgggagac ggcgttttcc agcacggcca ccatacagtc gagcatggcg acgtcgacga
      961 gctggcctct gcctgtcttc atgcgcgagt tcaccgcgga gagaataccg atggtggtga
     1021 aaaggcccgc gaagatgtcg gcaatcgagc tgcccacctt cgtgggagtg tgctcgtcgg
     1081 gaccggtgac gctcatcatg ccgcccatac cctggataat gacatccatt gcgggacgtc
     1141 tgctgtaggg gccggtctga ccgaacccgg agcttgccgc atagatcagg cgaggattca
     1201 cctttctgag atcttcatac cccaggccga gcttttccat cgtccccggt ttgaaatttt
     1261 cgataaggat gtcgctcttc ttggcgagcg acttgaggat ttccttgcct ttttcgtgtt
     1321 tcagattcag cgtaacgctc ttcttccccc tgttgaggct catgaagtag gcgctctccc
     1381 cggcctggaa gggagggtag gcgcgggtat cgtcgccgct gttaggccgc tcgatcttga
     1441 tgacttcggc gcccatgtcg gcgaacatca tggctgcaaa cggccccgcg agcactctgg
     1501 tcatgtcgag aactacaagt ccttccaacg gtttcatagc aaatccacct ccgaaatgat
     1561 gaccggaaca aaaagcgcag gcttttcatc cggttcgaat ctctcgtctc tctccgaaaa
     1621 gtcgttccat gacaagaagc gatttccgga aaaaagatct tccaatgtga tacaacgcca
     1681 gagagacttc tcagtcttct tttactctca aactttggaa acgctcaaaa gcgctgaatc
     1741 agattttaca atggacttat tttatcataa aatatgtttg gagcagagga aatgcaatgt
     1801 catcacatga caaaaattca gccaacaacc cccaatggat gacatccttc aaacggggca
     1861 aaaagggaga gtttacatga tcgtcgccga cctgttgaaa aaaaacctgt tccctgactt
     1921 tcagatactc gccggtagcg gcggcgtcgg gcgcgaaatc agcgccgtct cggtcctcga
     1981 agctccggac gccgaccgct ggatgcgcgg cggggagttt ctggtgggaa gcggcttcgt
     2041 cttcaaggac gacccggaac agctcacccc cctcctccgc cggatgaacg agaagggagt
     2101 cgccgccatc ggattcaagc tcgaccgctt ccatcataaa ctgccggcga gcatgaccgc
     2161 ggaggcggac gcgcttcaga tacccattct tgaaattccg atgcactacc gctggaccga
     2221 catcatcgaa ctcattcaca ccatcctcgt ccaggagcgg ctgaaagagc gcccggacga
     2281 tctgacctct ttctgggagg agagcctcga ctacaagaag ctgatgtccg gattcgcgca
     2341 gaatctcggc agagatcttc tggtgcaggc gccccagctc gaattgaaca atctcttcct
     2401 cgccgacggg agcatcctcg ggggcgaagc cgtgcagaaa gccctggaca cgccgctcgt
     2461 ccaggagcag cctctcccca agaaggggca gatacactcc tgcgtggagg tgcgcaacag
     2521 cggaaccatc cggcgcttcg cggcctaccg tctgcgcctg gacactccca tctccatctt
     2581 catgatcctc tcgcccggag agctcgcccc gtcggccagg caggagcgga tgctgctccg
     2641 cgccctgacg atgctgcggg cttccgcgct ggagatcgcc attttatcaa gcaagaaagt
     2701 gttgaaaaag gaacgctttc tggaggggtt gtgcttcgga atgtactgcg atccgaacat
     2761 gatccggatc aacctgcaag agctcgggat tccgctctcg cttcccgtga cggtgctcat
     2821 cgtctccacg gccgacgggc tctccactcc gcggtggtcg tccccctccc agctgagcta
     2881 ccggttgggg aatacgtggg tcacgctcat cgacgaatcg gagacggccg catggaaaaa
     2941 gagcc
//
LOCUS       DAOPKU010000384         2901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_38928_length_2901_cov_4.436051, whole genome shotgun sequence.
ACCESSION   DAOPKU010000384 DAOPKU010000000
VERSION     DAOPKU010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38928_length_2901_cov_4.436051"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PK849_11060"
     CDS             <1..871
                     /locus_tag="PK849_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPE66706.1"
                     /translation="LWYWLAVPLRGIAERLGNVGWGKERSIRLYNGPISELEALSSVI
                     ERLATESFEKEELKKRYVTDLVQAQESTRGQLARELHDGALQSTVAAVKRIQLAKQSV
                     PSDANSPLRAHLDTAEEVVQTAAGEIRSYCETLSPSWVRLGLSSALEEIAGRLAQAYE
                     GVETDVDIGVELEAAVAALPEDHVLALARIFQEAASNSVRHGGARFIQVELRKEGDAL
                     LFSVEDDGSGFDADAYAGTDFEELRTTGHRGLANINERVRLLRGTMRIMSRPGESCRI
                     DILFPPPSPGETR"
     gene            966..1601
                     /locus_tag="PK849_11065"
     CDS             966..1601
                     /locus_tag="PK849_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE66707.1"
                     /translation="MVRILLADDHPLTRSGLAAWIAKEEGMQLVGETEDGESAWSAIL
                     ELHPDLAMLDIEMPDCSGIDIASRVLKRGLATNVLILTAYSAHQYVMASVRAGAKGFI
                     LKSAPLAQLRKAIFQTAAGEFYLDPSVSLARTEGDVEQLSERELEVLVLAGQGLPGHE
                     VARLLSITERTVAAHLTSVYGKLGAKNKTEAILLALKRGVILLDQLHLIDS"
     gene            1863..>2901
                     /locus_tag="PK849_11070"
     CDS             1863..>2901
                     /locus_tag="PK849_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HPE66708.1"
                     /translation="MLKRMKIGGKLTLAFGVLLLIFAGVGAMSWMNMREVQREAHALA
                     DEAVPEMVVAASVQQAAQSVMYEIRGYGYTYEPRYLEQGRQKLVEIRNRLKEATDLAA
                     KFPALVRLRENAAKASAAVDQYAALVDRTEAAVQAIAAARTRGDTDQQEFFQLAEAYL
                     ASQNEALEGHIQAGDGADRLRDRAKKINQINGIIDLGNSIQIANFQGQTTRNPALLEE
                     AMKTFDRVDAVLAEIKSTTAQQANLDQLDGIGKAGANYKAVLAEILKEWNLLEAIEKD
                     RGTAAASVLALADEVVRTGATNAGKIAESAVSSLGSTILVILIATVVAVLFGGIVAFL
                     MTRSLTVPLKRV"
BASE COUNT          588 a          940 c          917 g          456 t
ORIGIN      
        1 cctctggtac tggctcgccg tgccgctgcg gggaatcgcg gaacgcctgg ggaacgtcgg
       61 gtgggggaag gaacgctcca tccgtctcta caacgggccg atctccgagc tggaggcgct
      121 ctcctccgtc atcgaacggc tcgcaacgga gtcgttcgag aaggaggagc tgaaaaaacg
      181 ctacgtcacc gaccttgtgc aggctcagga gagcacccgg ggacagcttg cccgggagct
      241 gcacgacggc gcgctgcaat cgaccgtggc ggcggtcaag cgcatccagc ttgcgaaaca
      301 gtccgtgcct tcggatgcga actcccccct ccgcgcgcac ctcgacaccg ccgaggaggt
      361 tgtgcagacg gcggcgggag agatccggag ctactgcgag acgctctccc cctcctgggt
      421 aaggctgggg ctctccagcg cgctcgagga gatcgccgga cgtctcgcgc aggcgtacga
      481 aggggtggag accgacgtgg acatcggcgt ggagcttgaa gcggcggtcg ccgcactccc
      541 cgaggaccac gtgctcgcac tggcgcgcat ctttcaggag gccgcgtcga actccgtccg
      601 gcacggcggg gcgcgcttca tacaggtcga gcttcgcaag gagggggacg cgctcctctt
      661 ctccgtggag gacgacggct cgggcttcga cgccgacgcg tacgccggaa ccgactttga
      721 agagctgcgg acgacggggc atcgcggtct ggcgaacatc aacgagcgcg tccgcctgct
      781 ccgcggaacg atgcggatca tgtcacgccc gggagagagc tgccgcatcg atatcctctt
      841 ccctcccccc tcgccgggag aaacgcgttg acaggagaaa cggcgttcca gcaacagcgt
      901 cgagcctccg tggcgtcgaa atgcggcggc cgcacacaca atcccgttca gaaaggggct
      961 ttgccatggt acgtattctg ctcgccgacg atcaccctct gacccgctcc ggcctcgccg
     1021 cgtggatagc gaaggaggag ggaatgcagc tcgtcgggga aaccgaggac ggagagtccg
     1081 catggtcggc tattcttgaa ctgcaccccg acctggcgat gctcgacatc gagatgccgg
     1141 actgcagcgg catcgacatt gcctcgcgcg tgctcaagcg cggactggcc acgaacgtcc
     1201 tcatcctcac ggcctacagc gcgcaccagt acgtgatggc ctcggtcagg gccggcgcaa
     1261 aagggttcat cctcaagtcc gccccgctgg cgcagctccg caaggcgatc ttccagacgg
     1321 cggcgggcga gttctacctc gacccctcgg tctcgctggc ccggacggag ggcgacgtcg
     1381 aacagctctc cgaacgcgaa ctggaggtgc tcgtcctcgc cggacagggg ctccctggcc
     1441 acgaggtcgc acgtctgctc tccatcaccg aacggaccgt cgcggcacac ctcacatcgg
     1501 tctacggcaa gctcggcgcg aagaacaaga ccgaggccat cctgctcgcc ctcaagcggg
     1561 gcgtcatcct tctggaccag ctgcatctga tcgacagcta gagggagact cccccgcctc
     1621 cccttgcgcg catccccctc tttccaaaac atttcccctt tcactcagcg aattgtcagc
     1681 tgtaaatctt tacagtcgca agactgtcga tctccgtaat gcgtctcccg ccgattccgc
     1741 gcatacttac accggtgaat aaggctatcg cgaggctctt tctgcgccga cgtccgctgg
     1801 aagagcccgt gaaggacgga gagtccgtcc ggaaaaccat aaaaaaaggg aggaatccac
     1861 atatgttgaa gagaatgaag atcggcggaa aactcaccct tgcgttcggc gtcctgctgc
     1921 tcatcttcgc gggagtcggc gcaatgagct ggatgaacat gagggaagtg cagcgggagg
     1981 cgcacgcgct ggcggacgag gccgtgcccg aaatggtcgt ggcagcctcc gtccagcagg
     2041 cggcgcaaag cgtcatgtac gagatacgtg gctacggcta cacctacgaa ccgaggtacc
     2101 ttgagcaggg gcggcagaaa ctggtggaga tacgcaacag actgaaagag gccaccgacc
     2161 ttgccgcgaa gttccccgca ctcgttcggc ttcgggagaa cgcggcaaaa gcctccgccg
     2221 ccgtcgatca gtacgcggcc ctcgtcgacc gcacggaagc cgcagttcag gccatcgccg
     2281 cagcgcggac caggggcgac acggatcagc aagagttctt ccaactagcc gaggcatacc
     2341 tcgcatccca gaacgaggcg ttggagggac acatccaggc cggggacggc gcggaccggc
     2401 ttcgggaccg cgcaaagaaa atcaaccaga tcaacggcat catcgacttg gggaactcca
     2461 tccagatcgc caacttccag ggacagacca cgcgaaaccc cgctctcctg gaggaagcga
     2521 tgaagacgtt cgaccgggtc gacgcagtac tggccgagat caaatcgacg acagcgcagc
     2581 aggccaacct cgaccagctc gacggcatcg gcaaggccgg ggcaaattac aaggccgtgc
     2641 tcgcggagat actgaaggag tggaatcttc tcgaagcgat cgagaaagac cgcggaaccg
     2701 ctgcggcaag cgtgctcgcg ctcgccgacg aggtggtgcg gaccggagcg acgaacgccg
     2761 gaaagatcgc ggaaagcgcg gtttccagtc tcggctccac cattctcgtc attctgatcg
     2821 ccacggtcgt cgcggtgctc ttcggcggca tcgtcgcctt cctcatgacg cgcagcctca
     2881 cagttccgct gaagcgcgtg g
//
LOCUS       DAOPKU010000385         2898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_38987_length_2898_cov_4.376715, whole genome shotgun sequence.
ACCESSION   DAOPKU010000385 DAOPKU010000000
VERSION     DAOPKU010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38987_length_2898_cov_4.376715"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PK849_11075"
     CDS             <1..926
                     /locus_tag="PK849_11075"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HPE66709.1"
                     /translation="DLLSSFISLTVTGVLMATVFALLRAPDVALTQAIINSGLVTSLF
                     LVAYSRTQRTPPPDGECRECPFWLRWPGAALVSALFAAVLFRGLFVVSGERILGRASA
                     HYLSHTLEETGALNVVAAILLDYRAFDTLGETTVIFTAVFGISLLLSGGRYVHSGYGL
                     SFIVKRGMALLTPFILLYAASILFLGHLTPGGGFQGGSVFATVAILICVVYGTNFEAA
                     RISPKTKETFEAGGAVLFVFIGLLGIAWGGGFLANLSGPFSAGTPGSLFSGGNMLLLN
                     LAVGMKVGAGLSSIFYTMIKILELEDDSWPS"
     gene            914..1249
                     /locus_tag="PK849_11080"
     CDS             914..1249
                     /locus_tag="PK849_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HPE66710.1"
                     /translation="MAVMSFITVSLLFALGLFTVLSQRNVFKTVIGLTLMESAVLLLL
                     ISWAYIPGGSAPILPLQGKGVDPLPHSFTLTAIVIGACDIALAFAFIMKLHRFERSVN
                     VDDFRRLKG"
     gene            1323..2834
                     /locus_tag="PK849_11085"
     CDS             1323..2834
                     /locus_tag="PK849_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPE66711.1"
                     /translation="MIFPNYWMLFAILFPMTAAFILQLLHHFAPSKTGGWILLSCGIT
                     SLLTFGAAVSAISGVRVLRLGGWGADLGVLLVSDPLSAVFLFISAFGGAAAVWTTFTN
                     ISKSGPSAEQWRLCGLFFILWASLNGILTTGDLFNLFIFYEILAVAAYVLTGASKTRE
                     ALEAGLKYLVLGTVGALLILLATSMLYMGGGILNFALLSSVVGGIPRGALAVAAGFMI
                     AGLGLKMGMVPFHFWLPDAHSSASTGVSMLLSGVVVKASFYSLLRVTTLLFPAEQAGF
                     SIPDTLLVLGLLSLFTGHISAQRQTSIKRMLAWSTVAHMGYLLIGIGCASPLGVAGAL
                     LHSFNHAAAKSALFLGAGSFSTLSGSKEWRSFTGFGRRFPREGAAFLLLAASIIGLPP
                     LGGFWGKWMIVLAAMEKGRWVTAVAVELGTALSCVYYVPVLFRLFSPRRSEDTPAAGA
                     FHSGMLFIALGMTPLLLALFYFVPSLYEYLISAGEWVVSPALYRDAVLGSMIP"
BASE COUNT          442 a          833 c          843 g          780 t
ORIGIN      
        1 acgaccttct cagctcgttc atctcgctca ccgtgacagg ggttctcatg gcgacggtct
       61 tcgctctttt acgcgcgccc gacgtcgcgc tgacgcaggc cattatcaac tcgggacttg
      121 ttacatcgct gtttcttgtg gcatacagcc ggacgcaacg tacgcctcct cccgacggag
      181 agtgccggga gtgtcctttc tggcttcgct ggccgggagc cgcccttgtc agcgctctct
      241 tcgccgccgt gctcttccgc ggcttatttg tagtgtcggg cgaacgcatc ttggggagag
      301 cgtccgctca ctatctctcg catactcttg aagagaccgg ggcgctcaat gttgtcgccg
      361 ccatccttct tgactaccgg gccttcgata cgctgggaga gaccacagtc atttttacgg
      421 cggtcttcgg tatctcgctg cttttgtccg gcggaagata tgttcactcc gggtatggtc
      481 tctctttcat cgtcaagcgt ggaatggcgc ttctgactcc ctttattctt ctgtatgccg
      541 caagcattct cttcctcggc catctgacgc ccgggggtgg attccagggg gggagcgtct
      601 tcgcaacagt ggcgattctc atctgcgtcg tctacggcac gaacttcgag gctgcgcgca
      661 tctccccaaa gaccaaagag acgttcgaag cgggaggcgc ggtgctcttc gtcttcatcg
      721 gacttctggg aattgcgtgg ggaggaggct tcctggccaa tctttccggt ccgttttccg
      781 caggcacgcc cggttctctg ttcagcggcg gaaacatgct gctgctcaac ctggccgtag
      841 gtatgaaggt gggggcgggc ctttcctcga tcttctacac catgataaaa atactcgaac
      901 tggaggacga ctcatggccg tcatgagctt tattaccgtc tcgctgctct ttgcgctggg
      961 gctcttcacc gtcctttccc agagaaacgt tttcaagacg gtgatcggtt tgacccttat
     1021 ggaatcggcg gtgcttctgc tcttgatttc atgggcgtat atcccgggcg gctcggcccc
     1081 tattctgccg ttgcagggaa aaggggtgga cccgcttccg cattcgttta ccctgacggc
     1141 gatcgtcatc ggcgcgtgcg atatcgcgtt ggcctttgcc tttatcatga aactgcaccg
     1201 tttcgagcgc agtgtgaacg tggatgattt caggaggctc aaaggatgat gagagaaccc
     1261 ttgcgcttct tctctcctga agacatccct gtctgcgatg cagaccggag cttgttgcgg
     1321 ccatgatctt tccaaactat tggatgctgt tcgctatcct cttcccgatg acggcggcct
     1381 ttattcttca actgctgcat catttcgcac cctcgaagac cggaggatgg atccttcttt
     1441 cctgtggtat cacctcgttg ctgacgttcg gtgcggccgt ttccgcgatt tccggcgtca
     1501 gggtgctccg gctcggggga tggggcgccg atttgggggt tctccttgtt tcggatcctt
     1561 tgtcggcggt ttttcttttc atctccgctt tcggaggagc ggctgccgtg tggacgactt
     1621 ttacgaatat ctccaaatca gggccgtcag cggagcagtg gcgtctctgc ggtctttttt
     1681 tcatcctctg ggcttcgctc aacgggattc ttacaaccgg cgatctcttc aacctcttta
     1741 ttttttatga aattcttgcc gtagccgcct acgtccttac aggcgcctcg aaaacgcggg
     1801 aggccctgga agccgggctc aaatacctcg tcctggggac ggtcggcgcg ttgctgattc
     1861 ttctcgcgac gagcatgctg tacatggggg gcggaatact gaatttcgcg ctgctctctt
     1921 cagttgtcgg cggcattccg cggggagcgc tcgccgtcgc ggccggattc atgattgcgg
     1981 gactgggact caagatgggc atggtcccgt tccatttttg gctgcctgat gctcattcgt
     2041 cggcttcgac gggagtgagc atgctcctct ccggcgttgt cgtcaaggcg tctttttatt
     2101 cgctgctcag ggtgacgacg ctgctctttc cggctgaaca ggcaggtttt tcaataccgg
     2161 atacacttct tgtgctgggg ctgctctcgc tcttcaccgg gcatatttcg gcgcagcggc
     2221 agacgagcat caagcgcatg cttgcctggt cgacagtggc gcacatgggg tatctgctga
     2281 tcggcatcgg gtgcgcgtct cctctgggag tggccggggc gctccttcat tcgttcaacc
     2341 acgctgcggc aaaatcggcg ctcttcctcg gagccggaag tttttcgacg ctctccggca
     2401 gcaaggagtg gcgttccttt accggtttcg gacgacgttt tccccgcgaa ggcgcggcgt
     2461 ttcttctgct cgcggcgtcg atcatcggac tgccgccgct gggcggtttt tggggcaagt
     2521 ggatgatcgt tcttgcggcg atggagaaag ggcgctgggt gaccgccgtc gcagtggagc
     2581 ttggaacggc gctctcctgc gtctattatg ttcccgtgct ctttcgtctg ttctcgcccc
     2641 gccggagcga agatactcct gctgccggcg cctttcactc cggcatgctc tttattgcgc
     2701 tgggaatgac gccgcttctt ctcgcccttt tttacttcgt accgtcgttg tatgagtacc
     2761 tgatttcggc aggagagtgg gtcgtttcgc ccgcgctgta cagagacgcc gttctgggct
     2821 cgatgattcc ctgagccgaa ccctgcaagg gagtctggcc taatccaaaa aaggaggctg
     2881 agaggatgac acgtttca
//
LOCUS       DAOPKU010000386         2893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_39093_length_2893_cov_5.883721, whole genome shotgun sequence.
ACCESSION   DAOPKU010000386 DAOPKU010000000
VERSION     DAOPKU010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39093_length_2893_cov_5.883721"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PK849_11090"
     CDS             <1..640
                     /locus_tag="PK849_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPE66712.1"
                     /translation="DCTGLEMDLETGLLLQTRPAIGGNVMATIKTPERRPQMATVRPR
                     TFRIPEPSERKGELLTPGIPAALFESSIENLGFDRVEDGAANIADMDVVVSGGKGLKR
                     PESFRMLEELAELLGGGVGASRPTVEAKWMDYPHQVGLSGKVVAPKVYIAAGISGAVQ
                     HLAGMQTAGYIVAINRDPDASIFRVADLGLRGDLFDIIPRIIDRIKTEVNSR"
     gene            637..2070
                     /locus_tag="PK849_11095"
     CDS             637..2070
                     /locus_tag="PK849_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPE66713.1"
                     /translation="MSVQAYSPVTEQTVEELQKLIGASNVSADLEKRIAYSYDEVPRF
                     YWDKEFIAEVVVFPETTEDVSAVMRYANDRRIPVTPRGAGTGLSGGAVPFSGGIVLSF
                     EKMRAIIELDKKNLTITVEPGVVTAEITKAAEREGLLYAGDPCSGDASFIGGNVAENA
                     GGNKVVKYGTTGNSVLALEAVLPDGTATWFGGKRRKDATGYDFAHLIVGSEGTLAVVT
                     KIILKLLPLPGKVLDLLVPFPDVASAIDFAPRVMSEGKVIPSSMEFMDRRSIKLAERF
                     LNTGLPFDDAGAHLIIQLEGNDPEVLADDMERIGDLCFAHGALEVFVADNRTTRDKLW
                     KARKCLAEAIMAFYPKYSLEDLVVPTSEIPKLMAETTRLCTKYGLEEANFGHVGDGNM
                     HVNILFPEERPDWHGIVDELLDELYDFTASIGGTLSGEHGIGLKRQKYMCRVMDASQM
                     ELIHRVKLAFDPNNILNPGKMISCPDR"
     gene            complement(2125..>2893)
                     /locus_tag="PK849_11100"
     CDS             complement(2125..>2893)
                     /locus_tag="PK849_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPE66714.1"
                     /translation="ILRPFLLRRTKDQVLDELPPKTEITLRVDMNPEERSLYEALRRN
                     ALERIEKGDGEDQRFVILAELMKLRRACCNGALVLPDREFPSAKLEAFGEILEDLRAN
                     GHRCLVFSQFVDHLAILRASLNEAGVSYSYLDGSTPPEERKRQVEAFQAGEKECFLIS
                     LRAGGTGLNLTAADYVVHMDPWWNPAVEDQASDRAHRIGQERPVTVYRIVAKDTIEEK
                     IVDLHTTKRDLAESLLEGTERVTSFSLNEMAALISQE"
BASE COUNT          548 a          866 c          942 g          537 t
ORIGIN      
        1 ggactgcacc ggccttgaga tggacctcga aaccgggttg ctgctccaga cccgtccggc
       61 tatcggcggc aacgtcatgg cgaccatcaa gacgccggag cgtcggccgc agatggcgac
      121 ggtgcgcccg cggacgtttc ggattccgga gccttccgag cggaaggggg agttgctgac
      181 gcccgggatt cctgccgctc tcttcgagag ctccatcgag aacctcggtt ttgaccgggt
      241 ggaggacggc gcggcgaaca tcgcggatat ggacgtagtc gtctcgggag gcaaggggct
      301 gaagcgcccc gagagcttcc ggatgctcga agagcttgcg gagcttctcg gcgggggcgt
      361 cggggccagc cgtccgacgg tggaggccaa gtggatggac tatccgcacc aggtcgggct
      421 ctcaggaaag gtcgtcgccc cgaaggtcta catcgcagcc gggatttccg gagccgtgca
      481 gcatctcgcg gggatgcaga ccgccgggta catcgttgcg atcaaccgcg acccggacgc
      541 ctccatcttc cgggtggcgg atctggggct tcgaggggat cttttcgata tcatcccccg
      601 gattatcgat cgtatcaaaa cggaggtgaa ctccagatga gtgtccaagc atactcaccg
      661 gtcactgaac agacagtaga ggaactccaa aagctgatag gagcgtcgaa cgtctccgca
      721 gaccttgaaa agcgcatcgc ctactcctat gacgaagttc ccaggtttta ctgggacaag
      781 gagtttatcg ccgaggtcgt cgtcttcccg gagactaccg aggatgtctc cgcggtgatg
      841 cgctacgcga acgaccgcag gattccggtg acgccaagag gggccggcac aggcctctcc
      901 ggcggtgcgg ttcctttctc gggcgggatc gtcctctcgt tcgagaagat gcgggccatc
      961 atcgagctgg acaagaaaaa cctcacgatc acggtcgaac ctggcgtggt gacggcggag
     1021 atcacgaagg cggcggagcg tgaagggctg ctctacgccg gggacccgtg cagcggcgac
     1081 gcctccttca tcggcggcaa cgttgcggag aacgccgggg gaaataaggt agtcaagtac
     1141 gggacgaccg gaaactctgt gctcgccctt gaagcggtgc ttccggacgg cacggcgacc
     1201 tggttcgggg gcaagcgccg gaaggatgcc acggggtacg acttcgctca tttgatcgtc
     1261 ggctccgaag gaacgttggc cgtggtgacg aagatcattt tgaagctgct tcctcttccc
     1321 ggcaaagtcc tggaccttct ggttcctttt cccgacgtgg cttccgcgat cgacttcgct
     1381 cctcgcgtga tgagcgaggg gaaggtgatt ccgtcgtcga tggagttcat ggacagacgc
     1441 tcgatcaagc tggcggagcg attcctcaac actggcctcc ccttcgacga cgcaggggcg
     1501 cacctcatca tccagctcga agggaacgat ccggaggtgc ttgccgacga catggagcgc
     1561 atcggggatc tctgcttcgc gcacggcgcc ttggaggtct tcgtcgcgga caaccggacc
     1621 acccgagaca agctctggaa ggcgcgaaag tgcctggcgg aggcgatcat ggccttctac
     1681 ccgaagtaca gtctcgaaga cctggtggtg cccaccagcg agataccgaa gctgatggcc
     1741 gagacgacgc gcctctgtac gaaatacggc ctggaggagg cgaacttcgg ccacgtgggg
     1801 gacggcaaca tgcacgtgaa cattcttttc cccgaggagc gccccgactg gcacggcatc
     1861 gtcgacgaac tcctcgacga gctgtacgac tttacggcct ccatcggcgg cacgctgagc
     1921 ggcgaacacg gtatcggcct gaaacggcag aagtacatgt gccgcgtgat ggacgcctcg
     1981 cagatggagc tgatccacag agtgaagctc gccttcgacc cgaacaacat cctcaacccg
     2041 ggcaagatga tcagctgccc ggatcggtag taggacacac cggagaagcg gaacgcaacg
     2101 ggggcccgag aaggcccccg ttgttcattc ctggctgatg agcgcggcca tctcgttgag
     2161 cgagaagctg gtcacgcgct ccgtcccttc gagcaggctt tcggcgagat ctcgcttcgt
     2221 cgtgtgaagg tccacgatct tctcctcgat ggtatccttc gccacgatgc ggtagaccgt
     2281 cacgggacgc tcctgcccga tgcggtgcgc acggtcggag gcctgatcct cgacggcggg
     2341 gttccaccag gggtccatgt ggacgacgta atcggcggcc gtgaggttca gccccgttcc
     2401 tccggcgcgc aggctgatga gaaaacactc cttctccccg gcctggaacg cttccacttg
     2461 gcgtttccgt tcctcgggcg gggtcgaacc gtcgaggtat gagtacgaaa ctccggcttc
     2521 gttcagcgat gctcgcagga tggccagatg gtcgacgaac tggctgaaga caaggcagcg
     2581 gtgtccgttt gcccgaaggt cttccaatat ctcgccgaac gcctccagtt tcgcggaggg
     2641 gaactctctg tcggggagga cgagcgcgcc gttacagcat gcgcgccgca gcttcatcag
     2701 ctcggcgagg ataacgaagc gctgatcctc tccgtctccc ttctcgattc tttcgagcgc
     2761 gttcctcctg agcgcctcgt agaggcttcg ctcctccggg ttcatatcca cgcggagggt
     2821 gatctccgtc ttcggaggca gctcgtcgag cacctgatcc ttggtgcggc ggagcaggaa
     2881 ggggcggagg atc
//
LOCUS       DAOPKU010000387         2892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_39109_length_2892_cov_4.110328, whole genome shotgun sequence.
ACCESSION   DAOPKU010000387 DAOPKU010000000
VERSION     DAOPKU010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39109_length_2892_cov_4.110328"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK849_11105"
     CDS             complement(<1..222)
                     /locus_tag="PK849_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPE66715.1"
                     /translation="MKNCPGCGAVIQEDAAFCEYCGVAVKQRSEAGKEGSASSWPVEG
                     TWSAAPEKTSGASFPQARLKKVSPLIMIFF"
     gene            400..1041
                     /locus_tag="PK849_11110"
     CDS             400..1041
                     /locus_tag="PK849_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPE66716.1"
                     /translation="MRSRSRKLDEEKRRNLMDAAIEEIAANGIEGASYNRIIERSGLS
                     KGVVYYYFENKESLYKTVLEEVERQFIAAVGTLRIPDDKDAFWDVCDMHYTKALKFAA
                     SNLRIVDVARSFIEPACSPSMDKGPVFESLQRAERWTRCVLRKGQELGALRQDVDFEL
                     LRNVVQAVGYTMDSWLFDKLRSEPDAVDIERFVSFALDMFKRILSPKGLGESC"
     gene            1035..2156
                     /locus_tag="PK849_11115"
     CDS             1035..2156
                     /locus_tag="PK849_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPE66717.1"
                     /translation="MLKKFFSRLSVLFWVVLLCAGGVLMVLRVQARLEESAANAAAME
                     QQEETAVPVDVQKLVRRDWEVWRSYYGQAKAGRSQTVSSYVKEIVRDVHVRIGDKVKA
                     GQVLLTLSSQDQRATEAALRSSYEDSVREYQRLSELQKSGGVSKSQVEQALSRMKSEE
                     ARLQASRSTLSRTQLRASIDGIVASRMIEPGEVAGENKPLLTVIDLKDLEAEIMVSRR
                     DIMSLSTETPVEVVSDGARAMGVIRRISPEAAPGSGLYTVVVGLNGLDILPGTHVEAR
                     FLVERRENLLVVPSDIVQRRGDKAYVYVVEGDRAKLREIVPGEGQNGSLAVTEGLAEG
                     ELLVTRGQNLLSDGALTRISGTGAPEAASSDIAEKTAEK"
     gene            2159..>2892
                     /locus_tag="PK849_11120"
     CDS             2159..>2892
                     /locus_tag="PK849_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPE66718.1"
                     /translation="MGGLVRFCIKRPVFTWAMVLVLMLLGVSSYNKLAVALLPKVDIP
                     IVVVRTVYRGASPNEIEQLVSKPIEDAIADLEGLDKITSYSAEGMSFVVLEMEVQVDV
                     DQALVDISNKVKAARNKLPDGVDEPIFSKVDINASPFMIVSFTSTLAEKDARKIIEDT
                     VVPILSRVDGVGQTDVTGGRNRQIRILLDPVSLSEYGVSIQTLAAVVASNNVTNPSGY
                     ITQTKDETWLRLVGEFNKVDELENIM"
BASE COUNT          612 a          762 c          932 g          586 t
ORIGIN      
        1 gaagaagatc atgatgagcg ggctgacctt tttgagcctc gcttgaggaa aggaagcgcc
       61 ggacgttttt tccggagccg cgctccacgt tccttcgacg ggccaggacg aagcggaacc
      121 ctctttcccc gcttcggagc gctgcttcac agcgactccg cagtactcgc aaaacgcggc
      181 gtcctcctgg atcacggcgc cgcatcccgg acagttcttc atagtgcatc gctccttgaa
      241 aaagaatcgg actgtttcat gccttcgtat cataccacag gcttcagagg cttggatgta
      301 tgtgaaattc cagttgatcg attctcctct tgacaaagta gaccggatgg tctaacctgt
      361 cagtcgcact tcacggtcga agtgttgagg aggtgcgatg tgcgttcacg ttccagaaaa
      421 ctggatgaag aaaaaagaag aaaccttatg gacgcggcca tcgaggagat agctgcgaac
      481 ggtatcgagg gggcgtcgta caacaggatc atcgaacgtt ccggattaag caagggagtg
      541 gtgtactact acttcgagaa caaggagagc ctgtacaaga cggtgctcga agaggtggaa
      601 cgtcagttta tcgccgctgt aggaacgttg agaattcccg acgacaagga cgctttttgg
      661 gatgtgtgcg atatgcacta cacgaaggcg ctgaagtttg ccgcttccaa tctgcggata
      721 gtcgatgtcg cccgcagttt tatcgagccc gcctgcagcc cctctatgga caaggggccg
      781 gtttttgaat ctctccagcg ggcggagcgc tggacaagat gcgtgctccg aaagggacag
      841 gagttgggag cgctgcgtca ggacgtcgat tttgaacttc tccggaacgt ggtgcaggct
      901 gtggggtaca cgatggactc gtggctgttc gacaagttgc gaagcgaacc ggatgcagtg
      961 gatatagagc gttttgtgag ctttgcgttg gatatgttta aaaggatttt gtcgccgaaa
     1021 gggttggggg agtcatgctg aaaaagtttt tttcgaggtt gagcgttctt ttctgggtcg
     1081 tacttctttg cgccgggggc gtcctgatgg tgctccgcgt tcaggcgagg cttgaagagt
     1141 ccgcggccaa cgccgcagct atggagcagc aggaggagac ggccgttccg gtcgatgtgc
     1201 agaagctcgt ccgacgcgac tgggaggtct ggcggagcta ctacggccag gcgaaggccg
     1261 ggcgcagcca gacggtttct tcctacgtca aggagatcgt ccgtgatgtt catgtgcgca
     1321 tcggcgacaa ggtgaaggcg ggacaggtgc tcctgacgct ctcctcgcag gaccagcgcg
     1381 ccaccgaggc tgcgctccgt tccagttacg aggattccgt aagggagtac cagcgtctgt
     1441 ccgagcttca gaaatcaggc ggcgtctcca agtcgcaggt ggagcaggcg ctctcccgca
     1501 tgaagagcga ggaggcgaga cttcaggcgt cgcgctccac cctctcgcgc acgcagctca
     1561 gggcgagtat cgacggtatc gtcgcctcgc gcatgatcga accgggagag gtggcgggcg
     1621 agaacaaacc gctgctcacc gtcatcgatc tgaaggatct tgaggcggag atcatggtct
     1681 ccagaaggga catcatgagt ctcagcacgg agactccggt agaggtcgtc tccgacggcg
     1741 cccgcgcaat gggcgttatc cggaggatca gtccggaggc ggcgccgggg tcgggactgt
     1801 acactgtcgt cgtcggcctc aacgggcttg acatacttcc aggaacgcac gtcgaggctc
     1861 gattcctcgt ggaacggcgg gagaacctgc tcgtcgtccc ctcggatatc gtccagcggc
     1921 gcggcgacaa ggcgtacgtc tatgtcgtcg agggcgacag ggcgaaactt cgagagatcg
     1981 tcccgggcga ggggcagaac ggatcccttg ctgtgactga agggcttgcc gagggagaac
     2041 tcctggtgac cagaggacag aacctgcttt ctgacggcgc gttgactcgg atctccggaa
     2101 cgggagcgcc cgaagcagcg tcatccgata ttgcggaaaa gacggctgaa aagtaatcat
     2161 gggcggtctt gtacgtttct gcataaagcg cccggtgttt acctgggcga tggtgctcgt
     2221 tctcatgctg ctgggggtga gcagctacaa caagctggcg gttgcgctgc tgcccaaggt
     2281 ggacatcccc attgtggtgg ttcggacagt ctacagagga gccagtccca atgaaataga
     2341 gcagctcgtc tccaagccga tcgaggatgc catcgccgac cttgaggggc tcgataagat
     2401 cacgagctac tctgccgagg gcatgtcctt cgtcgtcctt gagatggaag tgcaggtgga
     2461 tgtcgaccag gccctggtgg acatctcaaa caaggtgaag gcggcccgga acaagctgcc
     2521 cgacggggtc gacgagccca tcttcagcaa ggtcgatatc aacgcgagcc ccttcatgat
     2581 cgtctccttc acgagcacgc tggcggagaa ggatgcgcgg aagatcatcg aggatacggt
     2641 cgtccctatt ctctcccgcg tggacggcgt gggacagacc gatgtcaccg gaggccggaa
     2701 ccggcagata cggatattgc ttgatcccgt ttcgctgagc gagtacgggg tgagcatcca
     2761 gacgcttgcg gcggtcgtcg cgtcgaacaa cgtcacgaac ccctccgggt atatcacgca
     2821 gacgaaggac gagacctggc ttcgcctcgt cggcgagttc aacaaggtgg acgagctcga
     2881 gaacatcatg at
//
LOCUS       DAOPKU010000388         2888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_39192_length_2888_cov_3.971408, whole genome shotgun sequence.
ACCESSION   DAOPKU010000388 DAOPKU010000000
VERSION     DAOPKU010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39192_length_2888_cov_3.971408"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PK849_11125"
     CDS             complement(<1..835)
                     /locus_tag="PK849_11125"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE66719.1"
                     /translation="MLWLFLGILFFFTIFGVPIVFCLGIANIVMLLVMDIPLMALPAK
                     VIQGADSFPLLAIPFFMFVGEVMNRGGIARRLVDFADALVGHITGGLGHVSILSSMFF
                     GGICGSAVAETAAIGGLLIPPMKKQGFPAPLAAAICASAAVIGIIIPPSIPFILFGII
                     TSTSIARIFLGGIIPGIVVGLALMVTTYFITKKKGIARPAPDRKFSVKRVGQTLLSAG
                     WALSIPVIIVGGILGGIFTATEAGAISAFVAMILGLFVYKEISFKDLPGIFLSTGKTT
                     AT"
     gene            complement(840..1334)
                     /locus_tag="PK849_11130"
     CDS             complement(840..1334)
                     /locus_tag="PK849_11130"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE66720.1"
                     /translation="MKRLLQLGTALYRVALTVMTMALFIIVGVSVFTRYCLNSSLGWS
                     DELSRFIFIWVTFLGAAYAYGLDEHIGLDFVVDRIRSDRVRTVVRLLSEIAIGCVILV
                     ITWHGWEVAFSATNLSPALDIPMTWVYIVVPLTGCLMVVMNILKIGKWIGMLLATPSA
                     PAGR"
     gene            complement(1331..2620)
                     /gene="larA"
                     /locus_tag="PK849_11135"
     CDS             complement(1331..2620)
                     /gene="larA"
                     /locus_tag="PK849_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPE66721.1"
                     /translation="MARIAFPYDGFGDMVIPDENLLAVLAPRTATPPTGTDLDEVRRA
                     LAEPIASPGLKDMVKRGDSALLLIDDNTRNTPAARILPLVLEELAAGGVQIQDTAILI
                     ALGTHRHMTDAEVEAKVGAELAGRIRIFQHDAHEESGLAHIGVTEHGTDVWVNKLLLE
                     FDHVIAVGHITPHRVAGFSGGAKMVQPGVSGVVTTGQTHWISALYDGAEIMGTIDNPV
                     RREMNAVAKKAGLSFIVNVVLDREERIYRCVCGDPEKAHAAGCEASREIFGVLFPEYA
                     DIVVTDTFPADSELWQAAKGIYAGDLPLKRGGVLITVTPCPEGVAQGHPELTEIGYLP
                     FSEVKEMVDRNEIEDLTMAAHIVHVGRIIRDKGRGIMVCPCIDYATSKKLGFFPAATP
                     QQALDLAFSMTDPDATVIVLQNGGDLLPIHEPRRDAQ"
BASE COUNT          555 a          845 c          945 g          543 t
ORIGIN      
        1 ccgtggcggt tgtcttgccg gtcgaaagga agatgcccgg caggtctttg aaggagatct
       61 ccttgtagac gaagaggccg agaatcatgg cgacgaacgc cgaaatggcg ccggcctccg
      121 tcgcggtgaa gatgccgccg agaatgccgc ccacgatgat gaccggtatg gagagcgccc
      181 accccgcgct caggagagtc tggccgacgc gcttgaccga gaacttcctg tccggagcgg
      241 gtctggcgat ccccttcttc ttcgtgatga agtaagtggt caccatcagg gcgagtccga
      301 cgacgatgcc ggggatgatg ccgccgagga agatgcgggc gatcgaggtg ctcgtgatga
      361 tgccgaagag gatgaagggg atgctcggcg gaatgatgat gccgatgacc gccgccgacg
      421 cgcagatcgc cgccgcgagc ggggcgggga agccctgctt cttcatcggc gggatgagca
      481 gaccgccgat cgccgccgtc tcggctaccg ccgaaccgca gatgccgccg aagaacatgc
      541 tgctcaggat ggagacgtgc cccaggccgc ccgtgatgtg ccccacgagg gcgtccgcga
      601 agtccacaag cctgcgggcg atgccgcccc tgttcatcac ctcgcccacg aacatgaaga
      661 aggggatggc gaggagcggg aagctgtcgg cgccctggat gaccttcgcg gggagcgcca
      721 tcagggggat gtccatcacg agcagcatca cgatgttcgc gatgcccagg caaaagacga
      781 tgggaacgcc gaagatggta aagaagaaga gaatgccgag aaagagccag agcatggcgt
      841 tacctccccg ccggggcgga cggcgttgcg agcagcatgc cgatccactt cccgatcttc
      901 aggatgttca tgaccaccat caggcacccc gtaagcggga cgacgatgta gacccaggtc
      961 attgggatat cgagcgcggg agagaggttc gtcgcggaga aggcgacctc ccagccgtgc
     1021 caggtgataa cgaggatgac gcagccgatg gcgatctcgc tcaggaggcg gacgacggtg
     1081 cggactcggt cgctcctgat gcggtccacg acgaagtcca gtccgatgtg ttcgtccagt
     1141 ccgtaggcgt acgccgcccc gaggaaggtc acccagatga agatgaagcg cgacagctcg
     1201 tccgaccatc cgagcgagct gttgaggcag tagcgcgtga agacgctcac gccgacgatg
     1261 atgaagaggg ccattgtcat gacggtgagc gcgactctgt agagggcggt ccccagttgc
     1321 agcagccgct tcattgtgcg tccctccggg gttcgtgaat cgggagcaga tcgccgccgt
     1381 tctgcaggac gatcaccgta gcgtccgggt cggtcatcga gaaggcgaga tcgagcgctt
     1441 gctgcggcgt cgcggcgggg aaaaagccga gtttcttcga ggttgcgtag tcgatgcagg
     1501 ggcagaccat aatcccccgc cccttgtcgc ggatgatccg cccgacgtgg acgatatgcg
     1561 cggccatcgt caagtcctcg atctcgtttc tgtccaccat ttccttgact tcggagaagg
     1621 ggaggtagcc gatctccgtg agttccgggt gcccctgcgc gacgccctcg gggcaggggg
     1681 tgaccgtgat taaaacgccg ccgcgcttca acgggaggtc tcccgcgtag atccccttcg
     1741 cggcctgcca cagttcgctg tcggcgggga atgtgtccgt aaccacgatg tcggcgtact
     1801 cggggaacag aacgccgaag atctcccgcg acgcctcgca gcctgcggcg tgcgctttct
     1861 ccgggtcgcc gcagacgcag cggtagatac gctcctcccg gtcaaggacc acgttcacga
     1921 tgaaagagag ccctgctttc ttcgccaccg cgttcatctc gcggcgcacg gggttgtcga
     1981 tggtccccat gatctcagcg ccgtcgtaga gcgcgctgat ccagtgcgtc tggcctgtgg
     2041 tgaccactcc ggagacgccc ggctggacca tcttcgcccc tccggagaaa cctgcaaccc
     2101 tgtgcggggt gatgtgtccc actgcgatta cgtggtcgaa ctcaagaagc agcttgttca
     2161 cccagacgtc ggtaccgtgt tcggtgaccc cgatatgggc cagcccgctc tcctcgtggg
     2221 cgtcgtgctg gaatatccgt atgcgcccgg cgagttccgc gccaaccttt gcctccacct
     2281 cggcgtccgt catgtggcgg tgcgttccga gggcgatcag tatcgcagtg tcctgaatct
     2341 gcacgccgcc tgctgccaac tcctccagga caagcgggag gatacgggcg gcgggggtgt
     2401 tgcgggtgtt atcgtcgatg agcagcaacg ccgaatctcc ccgttttacc atgtccttca
     2461 gcccggggga ggcgatcggt tcggcaagag ctcgtcgtac ttcgtcaaga tcagttcccg
     2521 tcgggggcgt cgcagttcgg ggagcgagca ccgccagaag gttctcgtcg ggaatcacca
     2581 tatcgccgaa accgtcatag ggaaaggcga ttcgagccat ggcgcacctc cttctcgatt
     2641 ttcgcgggga tgtcgtcttg gtatattgct tcacctccga aaaatggcgc ccggaggagg
     2701 gtgttttgtc aggcgaaaca ctatcccgcg atttttttct atgttccatt ataatgcctt
     2761 ttcagtgttt tatgcgcgaa gagggcgaaa agattttgct ataagaaggc cgtatttcgg
     2821 aaggaagttt gctcgtgtta tgatgtgctc cggaggtgcg tacgatgtct gcggcaaaaa
     2881 gaagaaat
//
LOCUS       DAOPKU010000389         2886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_39229_length_2886_cov_4.395267, whole genome shotgun sequence.
ACCESSION   DAOPKU010000389 DAOPKU010000000
VERSION     DAOPKU010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39229_length_2886_cov_4.395267"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PK849_11140"
     CDS             complement(<1..1271)
                     /locus_tag="PK849_11140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPE66722.1"
                     /translation="MFLLRKKCFFRRALLLVSILSIVMLPQTAAARTVSVGLAIGQSS
                     VSVSSSSALSVTDGSGKKHALGTTAVFRSAGTNSVVAGNRTLRLPVSISSRHPLSFNK
                     RRYRGGFRLVSSGSGITLLNVLDLEEYLRGVLKMEVNPAWSMEALKAQAVISRTYALR
                     SVLQNRGKGGYDLRDTVMSQVYRGMNAEDPKTDQAIRATKGMVLKYGGEIAFTPFHSH
                     SGGATADVTTVWGGNMPYLKGVNEDFQTESPHSTWEASLSAAQVEDALRSAGVNVGRL
                     QELRVEQSDPFGRPTTLRAIGTSGSKTLKSHSFRMAAGSNVIRSTLFSISSAASSSSA
                     PIPLASLSQPKPVPPSPPPGTGVPTSLTPMSAAEERQLTDLTEQGVFNAEELMDMLLN
                     PEKRKGYLIRALRTVRPGIPVQPQPQPVGTAS"
     gene            complement(1331..2386)
                     /gene="ruvB"
                     /locus_tag="PK849_11145"
     CDS             complement(1331..2386)
                     /gene="ruvB"
                     /locus_tag="PK849_11145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPE66723.1"
                     /translation="MTEEIKRFLDPNDEPGEDENSLRPLSLHDFLGQEKLKEKLSIFI
                     EAASKRGEALDHTLFYGPPGLGKTTLAGIIAREMGGQLRVTTGPSLEKTGDLAAILSN
                     LEPNDVLFIDEIHRLPSNVEEILYPAMEDYALHIIVGKGPLANNICLTLPRFTLVGAT
                     TRLGLLTSPLRARFGIVEQLSLYSEDELRSIVARGARVLGVAIHDDAALEIARRSRGT
                     PRIALRLLRRVRDVAEVRALTSVDSATAFSALNMLGLDDLGLDEGDRRILRVIVELFD
                     GGPVGLSTLGAALNEETQTIEDIYEPFLIQQGLLERTPRGRKATKNCWIYLGKTPPPE
                     GNEQWQLHLPVEEGAEG"
     gene            complement(2398..>2886)
                     /locus_tag="PK849_11150"
     CDS             complement(2398..>2886)
                     /locus_tag="PK849_11150"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPE66724.1"
                     /translation="ASKKEPSPCRLHFSEAGPTLFGFADDRERALFLKLTTVKGVGGK
                     MAMNILKAAPAERIISWILSSDVDSMVKIQGIGRKTAERLCFEMRPHLTGEDAEIPGD
                     FFSSAGRKAETAMEALRSLGFTPADVGSVFRKLKESGALEGDDLTEEKILRLALKELK
                     RN"
BASE COUNT          675 a          833 c          742 g          636 t
ORIGIN      
        1 gaagccgtac cgacgggttg cggttggggc tgtacaggga taccgggacg tacagtacgc
       61 agtgcacgga taaggtaccc ctttcgcttt tcagggttga gcaacatatc catcagctct
      121 tcagcgttga aaaccccttg ctcggtaaga tcggtcagtt gtctttcttc cgccgcgctc
      181 atcggcgtca gcgacgtggg gacgcccgtt cctgggggag gcgaaggggg aacaggcttc
      241 ggctgagaaa gcgaagcaag gggaatagga gccgaagagg aggatgctgc ggacgagatg
      301 gaaaagagcg tactcctgat cacattgctc cctgcggcca tacggaagct gtgactcttg
      361 agcgtcttgc tcccgctcgt cccgatggcg cggagcgttg ttggcctgcc gaacggatcc
      421 gactgctcca cccgaagctc ctgaagacgc cctacgttca ctcctgcaga acgaagagcg
      481 tcctcaacct gggccgctga aagactggct tcccatgttg aatgcggaga ctccgtctga
      541 aaatcttcat tgacaccctt gagatacggc atgtttccgc cccacaccgt cgtcacatcg
      601 gcagtcgctc ccccgctatg cgaatggaaa ggggtgaatg cgatctcccc gccgtatttc
      661 aacaccattc cctttgtcgc gcgtatagcc tggtcggtct tggggtcttc agcgttcatg
      721 ccgcggtaga cctgcgacat aacggtatcg cgaagatcgt accccccctt gcccctgttc
      781 tgaaggacgc ttcgaagagc atacgttctt gagatgactg cctgcgcctt gagcgcttcc
      841 atggaccagg caggattcac ctccatcttc aacactcccc gaaggtactc ctcaagatcg
      901 aggacgttca gaagcgtaat ccccgagcct gaagatacca ggcggaatcc gcctcgatac
      961 cgtcttttat tgaaagaaag cgggtggcgc gaggagatac tcacaggcag acgtaacgta
     1021 cggtttcccg cgacaacact gttcgttccc gcactacgga atactgctgt tgtacccagc
     1081 gcatgttttt tcccgctccc atccgtcacg gaaagagcag aagaggagga aaccgaaaca
     1141 gatgactggc cgatagcgag accgacagaa acagttctgg ctgccgccgt ctgcggcagc
     1201 ataacaatac tcagaattga aactagaaga agagctcggc ggaagaaaca cttttttctc
     1261 agtaagaaca ttggcttacc cccatttttc ttatttcatc aatccaggac tactattttt
     1321 cacggagaat tcatccttca gccccctcct caacaggaag atgcaactgc cactgctcgt
     1381 tcccctcggg cggaggtgtt ttcccgagat atatccaaca atttttcgtc gctttccgtc
     1441 cccggggcgt tcgctcaaga agcccctgct ggatcaaaaa aggttcgtag atatcttcga
     1501 tcgtctgggt ctcctcgttg agcgcggcgc caagcgtcga aaggccgaca ggtccgccgt
     1561 cgaacagttc gacgataact ctgagaattc ttctatcacc ctcatcaagc cccagatcat
     1621 ccaacccgag catattaaga gcagaaaaag ccgttgcgct gtcaacagat gtaagggcgc
     1681 ggacctccgc gacgtcgcgg accctgcgaa gaagacgaag cgcgatacgc ggagtgccgc
     1741 gcgaacgcct ggcgatctca agcgccgcgt cgtcatgtat cgctacaccg agcaccctgg
     1801 cgccacgcgc gacaatactc ctgagttcat cttccgaata gagagagagc tgctcaacta
     1861 ttccgaaacg cgcacgaaga ggcgaggtga gcaacccgag ccgagtcgtc gcgccgacaa
     1921 gggtaaacct cgggagcgtc agacatatgt tgttcgccaa aggccccttt cccacgataa
     1981 tgtgcaacgc gtagtcctcc attgcggggt acaggatctc ttccacgttt gaagggagcc
     2041 tgtgaatctc gtcaatgaaa agaacgtcgt tcggctcaag attcgacaaa attgccgcaa
     2101 gatctccggt tttttccagc gaaggtcccg ttgtcacacg cagctggcct cccatttccc
     2161 tggcaatgat acctgcaagg gtggtcttac caagaccggg cggaccgtaa aaaagagtgt
     2221 gatccaaggc ctccccccgt ttggaggcgg cttcgataaa aattgagagt ttttccttca
     2281 gtttttcctg tccgaggaag tcgtgtaaag agagaggacg aagagagttt tcatcctctc
     2341 cgggttcatc gtttggatcc agaaagcgct ttatttcttc tgtcactgat tatcacctca
     2401 gttgcgcttt aattctttca gggcaaggcg caaaattttc tcttcggtaa gatcgtcccc
     2461 ttcaagagcc ccggactcct tgagcttccg aaaaacagat cccacatccg ccggcgtaaa
     2521 cccaagtgaa cgaagcgcct ccatagcggt ctctgctttt ctacctgcgg aagaaaaaaa
     2581 gtcccccggg atctccgcat cctctccggt aagatgagga cgcatctcga aacaaagacg
     2641 ctcggctgtc tttcttccta tcccctgaat cttgaccatg gagtcaacgt cagacgagag
     2701 aatccacgaa atgatacgtt ctgcgggcgc agcttttaaa atattcatcg ccatcttccc
     2761 gccgactccc ttcacggtgg tgagcttcaa aaaaagagcc ctctccctgt cgtctgcgaa
     2821 tccaaagagc gtcggccccg cctccgagaa atgcagacga cagggagagg gctctttttt
     2881 tgaagc
//
LOCUS       DAOPKU010000390         2881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_39326_length_2881_cov_4.644020, whole genome shotgun sequence.
ACCESSION   DAOPKU010000390 DAOPKU010000000
VERSION     DAOPKU010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39326_length_2881_cov_4.644020"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PK849_11155"
     CDS             complement(<1..254)
                     /locus_tag="PK849_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPE66725.1"
                     /translation="MNRGFLPQKRHRAREMALQLLYALDLRRSQTPEQAFELFPGDED
                     PEILEYARVLTSGAWERRMDIDNLIREYVVGWRPERMVAV"
     gene            complement(259..741)
                     /locus_tag="PK849_11160"
     CDS             complement(259..741)
                     /locus_tag="PK849_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66726.1"
                     /translation="MYLFWKRTSLGSILVSQDGMCDFVNSLCEKPFKCAQVSLSSADN
                     ALFLVITMPKDYDSSAMVSLETKLRDTFEKTGLSVRVSWVELAKTSFVDNEALASLAK
                     KPVVWAIAVALFALLLKEGVATLLWSLFWGGAFYAGVLFFQSEMGAELVRKLRGMAGR
                     "
     gene            complement(842..1393)
                     /locus_tag="PK849_11165"
     CDS             complement(842..1393)
                     /locus_tag="PK849_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPE66727.1"
                     /translation="MDEMNKKLESEVLEEESAYEGVLGETGESEAVPEGMVAGVVHIS
                     EEVLSELAKKTLSSIDGVQPASPGIASKLGIGRKASEGVRVVLEDNGLHPMASVDVYI
                     LVKFGLRIPDLAWDVQEMIKKNIEEYTGYKVKAVNVNVQGIYFKNKVPAEAVAEEAKG
                     EVKQPEDSVPELDKSVQPEVEQL"
     gene            complement(1465..1893)
                     /locus_tag="PK849_11170"
     CDS             complement(1465..1893)
                     /locus_tag="PK849_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66728.1"
                     /translation="MSAREKIAYLKGLLDGLGPVENEGQGKIFRAVVDALDALSLEVA
                     EHEDLIQEQRELYEDLADDCALLDEDLDSLEKAFSDFSGEGDMDDEDDEDEDDEFDRN
                     YVSVTCPHCSYVFYYQPEEYEPDEALECPGCGKDFEQPKE"
     gene            complement(1914..2486)
                     /gene="efp"
                     /locus_tag="PK849_11175"
     CDS             complement(1914..2486)
                     /gene="efp"
                     /locus_tag="PK849_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPE66729.1"
                     /translation="MAQVVDTSDFYSGIKIRWQEGIWEVIDFQHHKMGRGGATVRTKM
                     KNLDTGTIIENSFRSGERFERIIFDEKPAQFSYKEGDSYIFMDLSTYDQLSIPADILG
                     NVVNFLMDDMEVQIEFFEGRIMGIEMPKSVELKVVDTPPSFKGDTVSGGGKPAKLETG
                     ITISVPIFVNVDDTIVVDTRSGQYLERAKK"
     gene            complement(2603..>2881)
                     /locus_tag="PK849_11180"
     CDS             complement(2603..>2881)
                     /locus_tag="PK849_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HPE66730.1"
                     /translation="KGQAGEEFPLTSNREVTTMIRVRDGEPFVVGGLHKDVKRTERGR
                     VPILSNIPLLGELFKYRKDIRDKSEVAMIVIPYILTTPDGPVEQFTLK"
BASE COUNT          666 a          891 c          660 g          664 t
ORIGIN      
        1 tccaccgcga ccatgcgttc gggacgccat ccaacgacgt attcacggat cagattgtct
       61 atatccatac ggcgctccca tgcgccggaa gtcaaaacgc gcgcgtactc caaaatctcc
      121 ggatcctcgt cgcctgggaa cagctcgaac gcctgttccg gggtttggct tctccggaga
      181 tcaagcgcat acaataattg aagcgccatc tcacgtgcac gatgacgctt ctgcggcaaa
      241 aaaccacgat tcactccatt acctccccgc catgcccctc agctttcgca caagttcagc
      301 gcccatctcc gactggaaga aaagaactcc cgcgtaaaat gcgccgcccc agaaaagact
      361 ccatagaagc gtggccacgc cctccttgag caggagggca aaaagagcga ctgcgatcgc
      421 ccatacgaca ggtttttttg caagcgaggc aagcgcctca ttatccacga acgacgtctt
      481 cgcaagctcc acccatgaca cccgcacaga aaggccggtc ttctcgaaag tatcccgcag
      541 ttttgtctcc agagaaacca tagcggatga atcgtaatcc ttcggcatgg tgatgacaag
      601 aaaaagagcg ttgtcggcag aggaaagaga gacctgcgcg catttgaacg gcttttcgca
      661 caaagaattg acaaagtcgc acattccatc ctgcgaaacc aggatactgc cgagggaagt
      721 tcgtttccag aaaagataca ttcgcatcac ccctgttcga agggggctct gaaactgaag
      781 gagccccctt caaggttcgt tgatgaacta tgaactgaaa aaagccgcat cgaagcgaat
      841 gtcagagctg ctccacttcc ggctggaccg atttatcaag ctccggaacg gaatcctcgg
      901 gctgtttcac ctcgcccttc gcctcttcgg caacggcttc ggcgggaacc ttgtttttaa
      961 agtagatccc ctggacgttc acgttcaccg ctttcacctt gtagccggtg tactcttcga
     1021 tgttcttctt gatcatctcc tgaacatccc atgcaagatc gggaatgcga agaccgaact
     1081 tcacaagtat atacacatcc acgctcgcca taggatggag cccgttatcc tcaagcacaa
     1141 cccgaacgcc ttcgctcgct tttcttccga tgccgagctt ggaggcgata ccggggctgg
     1201 cgggctgcac accgtcaatg gaggaaagcg tcttcttcgc aagttcggag agcacctctt
     1261 cgctgatatg cactacgccg gcaaccatac cctccggaac agcctcgctc tccccagtct
     1321 cgcccaaaac gccttcatac gcagattctt cttcaagaac ttcagattcc agctttttgt
     1381 tcatttcatc catttcgaga cctcccttcc agttttacac gcccggccgg cagcgaatta
     1441 aagctcccgg ccgaatgctt ccgtctattc tttgggctgc tcgaaatcct tcccgcagcc
     1501 agggcattca agagcctcgt ccggttcgta ctcctcgggc tgatagtaga agacgtagct
     1561 gcaatgaggg cacgttacgg aaacgtaatt ccggtcgaac tcgtcgtcct cgtcttcgtc
     1621 atcctcgtcg tccatgtcgc cttcgccgga gaaatcggag aacgcctttt ccagcgaatc
     1681 aagatcttcg tcaagaagcg cgcagtcgtc cgccaagtcc tcgtacagct cacgctgctc
     1741 ctgaatcaga tcctcgtgct ccgccacctc cagagagagc gcgtcgagcg catccacaac
     1801 agccctgaaa atctttccct gtccttcgtt ctcgaccgga ccaagaccgt cgagcaaccc
     1861 cttgagatag gctatctttt cccgtgcgct catctcgtca acctccctgc gttttatttt
     1921 ttcgcccgtt caaggtactg acccgagcgg gtgtcgacga ctattgtatc atctacattg
     1981 acgaaaatag gaactgaaat ggtaatccct gtttcgagct tcgccggttt gcctccgccg
     2041 gacaccgtat cgcccttgaa gcttggaggc gtgtcgacga ccttgagttc aacgctcttc
     2101 ggcatctcga tgcccatgat gcgcccctcg aaaaactcga tctggacttc catatcgtcc
     2161 atgaggaagt tcacgacgtt gcccagaata tccgcaggaa tagagagctg gtcataggtc
     2221 gagagatcca tgaaaatata gctgtcgccc tctttgtaac tgaactgcgc aggcttctcg
     2281 tcgaagatga ttcgctcgaa gcgctcgccg gagcggaaag agttttcgat gatcgtcccc
     2341 gtgtccaggt ttttcatctt cgtcctgacg gtcgctcctc cgcgccccat cttgtgatgc
     2401 tggaaatcaa tgacttccca tataccttcc tgccagcgga tttttatccc cgaataaaag
     2461 tccgacgtat ccacaacctg cgccataaca ctctacctcc caatatcggt tcaccacagt
     2521 ccaaaagctg catccactgc aacgcaggta tattatcaca tccggaagtc cttctcaaaa
     2581 aaacgctgca aaaaacaacg cgctatttta aagtgaattg ctccaccgga ccgtcgggcg
     2641 tcgtcagaat gtacggaatc acaatcatcg ccacctcgga cttatcgcgg atatccttcc
     2701 tgtacttgaa cagctctccc agaaggggga tgttgctcaa tataggcaca cgtccgcgtt
     2761 cggtccgctt cacgtcctta tgcaggccgc cgacaacgaa cggctcgccg tcgcgaaccc
     2821 gtatcatcgt ggtcacttcg cggttggatg tcagcgggaa ctcctcccct gcctgccctt
     2881 t
//
LOCUS       DAOPKU010000391         2880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_39344_length_2880_cov_5.463717, whole genome shotgun sequence.
ACCESSION   DAOPKU010000391 DAOPKU010000000
VERSION     DAOPKU010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39344_length_2880_cov_5.463717"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PK849_11185"
     CDS             <1..534
                     /locus_tag="PK849_11185"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HPE66731.1"
                     /translation="AGETPKLDVLPYDGRIPKEEKLRRVVENLLGGSKPADHVIALTD
                     VYTGGTSPDFLDAQDAKKKMRQWVGAEPRFHPHVAQYDFEAWLLPYWPTIQKLAKHNK
                     GAPGGNPETVNHHNPPAYRIKEIFEIGKCRESYVKPRDAARILRENDLSVAVAQCSEL
                     KAMINTILSLCGGPVIP"
     gene            741..1187
                     /locus_tag="PK849_11190"
     CDS             741..1187
                     /locus_tag="PK849_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66732.1"
                     /translation="MTKEELGAVYRDFLAEEGFSPFIEEDGDVTFKYEGKVHGISVDE
                     GDEEFFRLIFAPFWDIENEEERRKVHAAACEATAKVKVAKIFPLGDDVVGSVELFLPE
                     VDDFKPVFKRALRCLSAAVDAFVASMRRGEKAIVVIEDGAGASVDS"
     gene            1380..2441
                     /locus_tag="PK849_11195"
     CDS             1380..2441
                     /locus_tag="PK849_11195"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPE66733.1"
                     /translation="MADKDNAFRRMLGDKEMFLRFLRRFLRRDMPAVVEDLIESGDFS
                     LDDLILENITFIPPDLREKRSDVVYRIRRGTLEAYVYILIEHQSSVDFLMPFRMLSYM
                     VRLWDRCIEEAGEIARRKSFQLPPIMPVVFYDGERPWTAVKLFTHKVRNTEDFRGYVP
                     DFEYRLISLRDVAANDLLRPGDALGTLLYLAKPFKSENFFEVSERIRKFLLVFPDEEK
                     LLLARHLGGYLKMLAKREGLDADSAPEDMFENEEAENMLTYLEKEIRRYKREGREEGL
                     AEGREEGRQEGWREGRQEGRQEGREEGRQEGRQEGREEVLRQTVRRMLASGMAVSLVA
                     EILEFSEERVRQLAGESSD"
     gene            2550..>2880
                     /locus_tag="PK849_11200"
     CDS             2550..>2880
                     /locus_tag="PK849_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE66734.1"
                     /translation="MELLHRLGGESSREWAMRVVLQNIIHLVLPPGTPLSDKEIASFL
                     SLSRTPVREALVLLEEMRFVDVYPQKGTFVSLLDVDDIEEGRYIRKCLESDTLIQACR
                     SFSADGAI"
BASE COUNT          666 a          695 c          882 g          637 t
ORIGIN      
        1 gctggtgaaa cccccaagtt ggatgttcta ccctacgacg ggcgcatccc caaagaagaa
       61 aaattacgac gggtcgtgga aaaccttttg ggaggaagta aacccgctga ccatgtgatt
      121 gcgctgaccg atgtttatac cggcggaacg tctcccgatt ttctcgacgc ccaggacgcc
      181 aagaaaaaaa tgagacagtg ggtgggagca gagccgagat ttcatcccca tgtcgcccaa
      241 tatgatttcg aggcgtggct gttgccgtac tggccgacga ttcaaaagct ggcaaaacat
      301 aacaaaggcg cccccggcgg caatcccgaa acggtgaatc atcacaaccc ccctgcatac
      361 cgaatcaagg aaattttcga gatcggcaag tgcagggaaa gctatgtcaa gcctcgcgat
      421 gcagcgcgga ttctaaggga aaacgacctc tccgtcgccg tcgcccaatg ctccgaactc
      481 aaggcaatga tcaacacgat tctttctctg tgcggaggcc cggtcatccc atgaacatgg
      541 ccgtttctct ggttgccgaa atactggagt tgttttggcg ctttcgatca acatttctct
      601 gggaataccc tctaaacggt cgcctccggt ctgtatgatg gttggtgtgg agagagttcg
      661 tcctgatcgg atgcgaagct tcaccaaaac ggctgtgata ttcagtattt cggacggtat
      721 cggagaggag cgttgttgcc atgacgaaag aagagcttgg tgcggtgtat cgggattttc
      781 tggccgaaga ggggttctct ccctttatag aagaagatgg agatgttacg ttcaaatacg
      841 aaggaaaagt tcatggcata tcggtcgacg agggcgacga agagtttttc cgtctgattt
      901 tcgctccctt ttgggacatc gaaaacgagg aggaacggcg aaaagtgcac gccgccgcct
      961 gcgaggccac ggcgaaggtg aaggtcgcca agatttttcc tcttggagac gacgtcgtcg
     1021 gaagcgtcga actgtttttg ccggaggtcg atgacttcaa gcccgtgttc aagagggctt
     1081 tgcgctgctt gtcggcggcg gtcgacgcgt tcgttgcgtc gatgagaaga ggtgaaaagg
     1141 cgatagtcgt gatagaggac ggagccgggg cgtccgtcga ctcctgagaa tatccgtccg
     1201 cgtggaagct gctcaagctc gcaaaagcag cttccacgga caaggaatcg gacaggtatg
     1261 cggcgcgggg cctgccgcga atccaggaat ttgcgtgata gcggatggta ccggtgaaga
     1321 agagggcttt tctccacatg ctataataat caaaaatttc atcgtcgagg gagagctgta
     1381 tggctgacaa ggacaacgct ttcaggagaa tgctcgggga caaggagatg ttcctccggt
     1441 ttctccgccg ttttttacgg cgcgatatgc ctgcggtcgt cgaggatctg atagagtccg
     1501 gagatttttc tctcgacgat cttatcctgg agaatatcac gttcatcccg ccggatctcc
     1561 gggagaagcg gtcggacgtc gtctatcgca tccggcgtgg aacgcttgaa gcgtacgtct
     1621 acatattgat agaacatcag tcgagcgtcg actttctgat gccgtttcga atgctgtcct
     1681 acatggtgcg cctgtgggat cgctgcatag aagaggccgg ggagatcgcc aggaggaaaa
     1741 gcttccagct tcctccgatc atgcccgtgg tcttttacga cggggagagg ccatggacgg
     1801 cggtgaagct ttttacgcac aaggtccgaa acactgagga ttttcgggga tatgttcccg
     1861 atttcgagta tcgtctgatc tccctccgcg acgtggccgc caatgatttg ctgcggccgg
     1921 gtgacgcgct gggaacgctt ctctatctgg cgaagccgtt caaaagcgag aactttttcg
     1981 aggtgtccga gaggatacgg aagtttctgc tggtctttcc ggacgaagaa aagctgctcc
     2041 tggcgagaca cctcggcggt tatttgaaga tgctggcgaa gcgagagggc ttggatgccg
     2101 atagcgcgcc ggaggatatg ttcgaaaacg aggaggctga gaacatgctg acctacttgg
     2161 agaaagagat acggagatat aagcgcgagg gccgcgagga agggttggcg gaagggcgag
     2221 aagaaggacg gcaggagggg tggcgagaag gacgtcaaga aggacgtcaa gaaggacgtg
     2281 aagaaggtcg tcaggaaggt cgtcaagaag gacgtgaaga agtccttcga cagaccgtcc
     2341 ggcgcatgct tgcctcgggc atggccgttt ctctggtcgc agaaatactg gagttctccg
     2401 aagagcgggt gcgtcagctc gccggagaga gttcggactg atttcgagcg tctcaggagg
     2461 aggcattatc ctgagagatt tttcttgaca aagccgcaac tgatatatta gtatgactag
     2521 catataaaac tacgatgagg agggggagca tggaattact gcaccgtctc ggcggcgagt
     2581 cgtccaggga gtgggcgatg cgcgtcgttc tccagaacat cattcatctt gtccttccgc
     2641 cggggacgcc gctctccgac aaggagatcg cctcctttct ttcactgagc cggacacccg
     2701 tccgcgaagc tcttgtcctt cttgaagaaa tgcgttttgt cgacgtctac cctcagaagg
     2761 ggacgttcgt ctccctgctc gacgtggacg atatcgagga ggggcgctat atccgcaagt
     2821 gtctcgagtc cgatacgctg attcaggcgt gccgctcatt ttcagccgac ggggcgatcg
//
LOCUS       DAOPKU010000392         2880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_39349_length_2880_cov_4.438584, whole genome shotgun sequence.
ACCESSION   DAOPKU010000392 DAOPKU010000000
VERSION     DAOPKU010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39349_length_2880_cov_4.438584"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PK849_11205"
     CDS             <1..1109
                     /locus_tag="PK849_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPE66735.1"
                     /translation="HKIEVLVVSDPANMNYLTGYDGWSFYVHQGLVVALDRDEPLWWG
                     RGMDGNGAKLTTWLRHDSIRPYEDDYVQNVFKHPMSFVADLIREHGWERKTLGVEMDN
                     YWFTGKCLETLAEELPAMEIADATSLVNWVRVIKSPAEIAYMKQASKVCEHVMRTAVD
                     SIKVGAPESSAAANVYHACITGTPEFTGDHPAIFPIMPSNERTSCAHLGWDPERAYAP
                     GDIVLLELCGVRFRYHCPLSRTVYIGTPHEKLRKVADTVLKGVEETLAFIKPGVVAEE
                     VEARWRKAIEGSGVVKPSRLGYSIGANYVPDWGEHTLSLRPGDKTVLKPGMTIHLMPG
                     IWQDDFGFENSEPFLVTETGCETLCSFPRQIFTR"
     gene            1118..2302
                     /locus_tag="PK849_11210"
     CDS             1118..2302
                     /locus_tag="PK849_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE66736.1"
                     /translation="MATIGPETQRLAKELVALRREFHARPELGFQEHWSASRIAGYLR
                     ELGLDVREGVAETGVTALLEGSGPGRTLLLRADMDALPLQELTGLPFASANDGVMHAC
                     GHDAHMAVLLVAAKALCGMRDRFAGRIRFVFQPNEEVAGARRMIDEGRLLDDPPVDGA
                     IALHVWSGIPSGKISIQPGPVMAAMDEFRVAIRGKGGHTGAPHKAVDPVLAAANFISA
                     AQMIQSREVDVLSPTLVMFGAVHAGGQASNIVPEKVELAGTVRYLYEGGPDSPERPLQ
                     RFERILAGICAASGTEFTCSWIPSNPCLVNDPAMAAVVREAAVSIVGEENIVPFISTA
                     GEDFAEFARSVPSAFAFVGTGNAAKGTDYPQHHPKFDVDEEALPAAAELFVRSALAFL
                     AK"
     gene            2347..2742
                     /locus_tag="PK849_11215"
     CDS             2347..2742
                     /locus_tag="PK849_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HPE66737.1"
                     /translation="MKKQPVHPASAPVPAGAYTPGLIAGGFVFVSGQTAEKPGSAELV
                     EGGIREQTKQVMENIGGILEAAGCSMDDVVKVTAHLADVRDFDGYNEVYREFFSTPYP
                     VRTTVQSVIPGGSLVEIDVVALRPERKGL"
BASE COUNT          487 a          995 c          965 g          433 t
ORIGIN      
        1 ggcacaagat cgaggtcctc gtggtgagcg accccgcgaa catgaactac ctcaccgggt
       61 acgacggctg gtccttctac gtccaccagg ggctcgtggt cgccctcgac cgggacgagc
      121 cgctctggtg ggggcgcgga atggacggca acggggcgaa gctcaccacc tggctgcgtc
      181 acgacagcat ccgcccctac gaggacgact acgtccagaa cgtcttcaag cacccgatga
      241 gtttcgtcgc cgacctgatc agggagcacg gctgggagcg caagaccctc ggcgtcgaga
      301 tggacaacta ctggttcacg ggaaagtgcc tggagacgct ggcggaggag cttcccgcga
      361 tggagatcgc ggacgccacc tcccttgtga actgggtgcg ggtaatcaag tcgcccgccg
      421 agatcgcgta catgaagcag gcgtcgaagg tctgcgagca cgtcatgcgc accgccgtcg
      481 actccatcaa ggtcggcgcg cccgagagct ccgcggcggc caacgtctac cacgcctgca
      541 tcaccggcac tcccgagttc acgggcgacc accccgccat cttcccgatc atgccgtcga
      601 acgagcggac ctcctgcgcc cacctcggct gggaccccga gcgcgcctac gcccccggcg
      661 acatcgtgct gctggagctg tgcggcgtgc gcttccgcta ccactgcccg ctgtcgagga
      721 ccgtctacat cggcacgccg cacgagaagc tgcggaaggt ggccgacacc gtcctcaagg
      781 gcgtggagga gacgctcgcc ttcatcaagc ccggcgtggt cgccgaagag gtcgaggcgc
      841 gctggcgcaa ggccatcgag ggctccggcg tggtcaagcc gtcgcgactg gggtactcca
      901 tcggggcgaa ctacgtcccc gactggggcg agcacacgct gagcctccgc cccggcgaca
      961 agaccgtgct caagcccgga atgaccatcc acctcatgcc cggcatctgg caggacgact
     1021 tcggcttcga gaacagcgag cccttcctcg tcaccgagac agggtgcgaa acgctctgct
     1081 cgttcccccg gcagattttc acccgctgag gcgcgtcatg gcgaccatcg gtccagaaac
     1141 gcagcggctc gccaaggagc tcgtcgccct caggagggag ttccacgccc gtccggagct
     1201 cggcttccag gagcactgga gcgcctcgcg gatagcgggc tacctccggg agctggggct
     1261 cgacgtccgt gagggcgtcg cggagacggg cgtcacggcc ctgctcgaag gaagcggccc
     1321 cggccggacg ctgctgctgc gcgccgatat ggacgcgctc cctctgcagg agctgaccgg
     1381 cctccccttc gcctcagcga acgacggcgt gatgcacgcc tgcgggcacg acgcgcacat
     1441 ggcggtcctc ctcgtcgcgg cgaaggcgct ctgcggaatg cgcgaccggt tcgccggaag
     1501 aatccgcttc gtcttccagc ccaacgagga ggtcgcgggg gcgcgccgga tgatcgacga
     1561 ggggcggctg ctcgacgacc ctcccgtgga cggcgcgatc gccctccacg tctggagcgg
     1621 catcccctcg gggaagatct ccatccagcc cggcccggtt atggcggcca tggacgagtt
     1681 cagggtcgcc atccggggaa agggcggcca cacgggcgcg ccgcacaagg ccgtcgaccc
     1741 cgtgctcgcc gcggcgaact tcatctccgc cgcgcagatg atccagagcc gcgaggtgga
     1801 cgtcctctcg ccgacactcg tcatgttcgg cgccgtccat gcgggcgggc aggcctccaa
     1861 catcgtcccc gagaaggtcg agctcgccgg aacggtccgc tacctctacg agggcggccc
     1921 ggacagcccg gagcgcccgt tgcagcgctt cgagcgcatt ctcgccggaa tctgcgccgc
     1981 ctcggggacg gagtttacct gctcgtggat ccccagcaat ccgtgcctcg tcaacgaccc
     2041 cgcgatggcg gcggtcgtgc gggaggcggc cgtctccatc gtcggcgagg agaacatcgt
     2101 ccccttcata agcaccgccg gggaggactt cgccgagttc gcgcgctccg tcccgtcggc
     2161 cttcgccttc gtcggcacgg ggaacgcggc gaagggaacc gactacccgc agcatcaccc
     2221 gaagttcgac gtcgacgagg aggcgcttcc ggcggcggcg gagctcttcg tccgctccgc
     2281 gctcgccttc ctcgcgaagt gagcgcatcg tcgacggatt ctacaggaaa ggagcgagcg
     2341 cgactcatga aaaaacaacc ggtccacccc gcgtcggccc ccgttcccgc gggagcgtac
     2401 acgccgggac ttattgcggg cggcttcgtc ttcgtcagcg gccagaccgc cgagaagccc
     2461 ggcagcgccg aactcgtcga gggcggcatc cgagagcaga cgaaacaggt gatggagaac
     2521 atcggcggca tcctggaggc tgcaggatgc tccatggacg atgtggtgaa ggtgacggcc
     2581 caccttgcgg acgtccgcga cttcgacggg tataatgagg tgtacaggga gttcttttcg
     2641 acgccctacc ccgtccgcac tacggtgcag agcgtcatcc cgggcggctc gctcgtggag
     2701 atcgacgtcg tcgccctccg ccccgaaagg aaggggctct gatggaggag cgggaggaac
     2761 gggcgcggca gcgttttccg aacgcttccg atgtgtggga ggcgcggaag cggatcgccc
     2821 cgtacatccg gaggacgccg cttgtcttct cgcaccggct ctccgaaatt tcgggcgcgg
//
LOCUS       DAOPKU010000393         2858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_39767_length_2858_cov_3.696753, whole genome shotgun sequence.
ACCESSION   DAOPKU010000393 DAOPKU010000000
VERSION     DAOPKU010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39767_length_2858_cov_3.696753"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PK849_11220"
     CDS             complement(<1..1291)
                     /locus_tag="PK849_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE66738.1"
                     /translation="MDIGYISVLLFGSMFLFLLLGLPVAFVLGGLATVFTAIFWGPES
                     LFIIVARTYSMMSTTTLVACPLFVLMATVLERSGVSEDLYDMMYRWMGSLKGGLAIGT
                     VIACTLIAAMSGIASTGVVMMGMMALPAMLKRGYDKRLATGCILAGGVLGPLIPPSIA
                     LVLYGTIAQVSIGALFAGGMSAGLLCSVLIIGYILIKCHLNPEMGPAIPEEERADWGA
                     KMKSLKGIFLPMLLVFSVLGSIYSGIATPTEAAAVGALGAIVCSALHGRLTWKLLRDA
                     SYATIKVQGFMMWILFAAQAFAAVYMGMGASRMVVGLVKEYQVGWWTMLIGIQVVWFL
                     LGFVIDAWSILMITSPIILPLLPIYGFNPLWLGVLYAVNTQTGYLTPPFGTMLFMMKG
                     IAPKGITMGDIYRSVIPFVITQLVCLALCIIFPPLITW"
     gene            complement(1301..1831)
                     /locus_tag="PK849_11225"
     CDS             complement(1301..1831)
                     /locus_tag="PK849_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HPE66739.1"
                     /translation="MIKFINFTDTLNDWVGKIFGFLVYPTMLVLVYEVVMRYGFNRPT
                     IWAHETSCMLYGAHFILGGAYALRWGGFVNVEAFYILFSPRVRAIIDLFTWTLFYAFV
                     GTLLWFSLPWAWESLQMMEYSNSTWGPYVWQLKLTIPAAAFLVLLQGMTKTIKDLHLA
                     LTGRELIPSAEAPVEN"
     gene            complement(1940..>2858)
                     /locus_tag="PK849_11230"
     CDS             complement(1940..>2858)
                     /locus_tag="PK849_11230"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPE66740.1"
                     /translation="TTYVIPPFIDMVREKSGGRLNITLHYAGELVDYLEVFPSLQANM
                     IQISNTSTLFWRGSLNVGWLQAGNLPPFVCRSLEDFEELYYKRGIDRLIGEGLAERDI
                     HFLGSHSVGNTYFWSKKPINTLDDLKGFKVRFFGAMNDTMEHFGASPVMLPHPETYMA
                     IAMGTLDGSGTAWWLYRDLKLYEVCPYFIGPAWQVPQGMELWVSKKAWDALPDDLKKV
                     VEEATVEFNKTFAKVCDEQEKEMFAKSFKEWGTTYIEWGKDDIDRITKEFSLPFLNKI
                     EQEIGSKDPRVAEGIQIIKQFMKDRGYIN"
BASE COUNT          649 a          836 c          815 g          558 t
ORIGIN      
        1 gccaggtgat aagcggcgga aagatgatgc acaacgccag acacaccaac tgcgtgatca
       61 cgaaggggat gacggagcgg tagatgtctc ccatcgtgat tcccttgggt gcgatgccct
      121 tcatcatgaa gagcatggtg ccgaagggcg gcgtcaggta tcccgtctgc gtgttcaccg
      181 cgtagaggac gcccagccac agagggttga atccatagat aggcagcagg gggagaatga
      241 tgggcgaggt gatcatgagg atgctccacg cgtcgatcac gaagccgagc aggaaccaga
      301 cgacctggat gcctatcagc atcgtccacc agccgacctg atactccttg acgagtccga
      361 cgaccatccg cgaagcgccc atacccatgt agaccgccgc gaatgcctgt gcggcgaaca
      421 agatccacat catgaatccc tgcaccttga tcgttgcgta cgacgcgtct ctgaggagct
      481 tccaggtcag tcttccgtgg agcgcgctgc acacgatggc gcccagagcg cctactgcgg
      541 cggcctcggt cggcgtggcg atgccgctgt agatcgaccc caaaaccgag aagaccagca
      601 gcatggggag gaagataccc ttgagggact tcatcttcgc cccccaatcg gcgcgttcct
      661 cttccggaat ggcaggcccc atttcagggt tgaggtggca tttgatcagg atgtagccaa
      721 tgatcagaac ggagcagagc aaccccgcgc tcatgccccc ggcgaaaaga gcgccgatgg
      781 agacctgagc gattgttccg tagagcacca gggcgatgct cggggggatc agcggtccaa
      841 gaacgccgcc cgcgaggatg cagcctgtgg ccagcctttt gtcgtacccg cgtttgagca
      901 tcgcgggaag ggccatcatg cccatcatga caacgccggt ggaggcgatg cccgacatgg
      961 ccgctatcaa ggtgcacgct atcaccgtgc cgatagcaag cccccccttg agagagccca
     1021 tccagcggta catcatatcg tacagatctt cagaaacccc ggagcgctcc agcacggtcg
     1081 ccatcaggac gaaaagaggg caggccacca gcgtcgtggt cgacatcatg gagtacgttc
     1141 ttgccacgat gataaagagg gactccgggc cccagaaaat tgcagtgaac accgttgcca
     1201 gtcctccgag gacgaacgcg acgggaagcc cgagcagcag gaagagaaac atgctgccga
     1261 agagaagaac ggaaatatat cctatgtcca taatagcacc tcagttctca accggagctt
     1321 cggccgaggg aataagttcg cggccggtca gcgccaggtg gagatccttg atggtcttgg
     1381 tcatcccctg gagcagcaca agaaacgccg ctgcgggtat ggtcagcttg agctgccaga
     1441 cgtacgggcc ccacgtgctg ttcgagtact ccatcatctg caaactctcc cacgcccacg
     1501 gcaaactgaa ccagagcaaa gttccgacga acgcgtaaaa aagcgtccac gtaaagagat
     1561 cgatgatagc gcgcacccgc ggtgagaaga gaatgtagaa cgcctctacg ttgacgaacc
     1621 ctccccagcg gagggcgtac gctcctccaa gaataaaatg cgccccgtac agcatacacg
     1681 aagtttcgtg cgcccatatc gtagggcgat tgaatccgta gcgcataacc acctcgtaca
     1741 cgagtacgag cattgtcggg tacacgagaa agccgaaaat cttccccacc cagtcgttca
     1801 gtgtgtcggt aaaattaata aacttgatca aaaagcccaa tcgcctcacc tcgccgattc
     1861 tgaaaaaatt gaaagcgcgc cccctcctcc ggagagagaa ggggggcgct tcgtggatgc
     1921 gtcgggtgga cgctccctgt tagttgatgt acccgcgatc cttcatgaac tgcttgatga
     1981 tctgaatacc ttcggcaacc ctcggatcct tcgagccgat ttcctgttcg atcttgttca
     2041 ggaaggggag agagaattct ttcgttatcc tgtcgatgtc atccttgccc cactcgatgt
     2101 aggtcgtccc ccactccttg aaggacttcg cgaacatctc tttttcctgc tcgtcgcaca
     2161 ctttggcgaa cgtcttgttg aattctactg tagcttcctc gacgaccttc ttcaggtcgt
     2221 cgggcagggc gtcccacgcc ttcttggaca cccacagttc cataccctga ggcacctgcc
     2281 atgcggggcc gatgaagtac gggcacactt cgtagagctt cagatcgcga tagagccacc
     2341 aggcggtgcc gctgccgtcg agagtgccca tcgcgatggc catgtaggtc tcgggatgcg
     2401 gcagcatgac cggcgacgcg ccgaagtgct ccatggtgtc gttcatcgcg ccgaagaaac
     2461 ggaccttgaa tcccttgaga tcgtccagcg tattgatggg ctttttactc cagaagtagg
     2521 tgttgcccac gctgtggctt cccaggaagt gaatgtcccg ctcggccagt ccttctccga
     2581 tgagtctgtc gatgcctctc ttgtagtaca gctcctcgaa gtcctcgagg ctccggcaga
     2641 cgaagggggg cagattgccc gcctggagcc aaccgacgtt cagcgagcct ctccagaaga
     2701 gcgtgctggt gttggagatc tggatcatgt ttgcctgcaa agaggggaac acttccaggt
     2761 agtctacgag ttctcccgca tagtgcaggg tgatgttcag tctgccgccc gacttctcgc
     2821 ggaccatatc aataaacggc gggatgacgt atgtcgtc
//
LOCUS       DAOPKU010000394         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_39810_length_2856_cov_4.677258, whole genome shotgun sequence.
ACCESSION   DAOPKU010000394 DAOPKU010000000
VERSION     DAOPKU010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39810_length_2856_cov_4.677258"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..832)
                     /locus_tag="PK849_11235"
     CDS             complement(137..832)
                     /locus_tag="PK849_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66741.1"
                     /translation="MRGWKRAFGSCALLLFFASAGWASSLIADYEARFTKYFTDRIQA
                     QSVLSVIKASEDELRRGKIPTLNLCIEDAVVEGVRYDRLLLVMHDVLFTSAARGIVVH
                     SHGESHLSGGISKETFLKTLSEGMPHFAVSELALRNGSVMIKGVYERRLTFKVRALMR
                     FTGSYVIDGDGVARVRFDDASNDNPAISTLDVAKAMANAASVLSFRDFFGAPLVREVL
                     VDHDMVWFSARSQ"
     gene            complement(887..1831)
                     /locus_tag="PK849_11240"
     CDS             complement(887..1831)
                     /locus_tag="PK849_11240"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPE66742.1"
                     /translation="MGRSGRFTFFRFGSILFLLFCLVCIPLSASAEVTVQYPSSVGVG
                     KPFLVRVTSSVPLTGVEAVWQGRTVRLGVSVWNERYISLALLGTQVGKVKTGDHRLTL
                     RLLSGGKRSEHPFSIKVMPVQYKEDRLTLPEKMVTPPSSELKRIEAERKRTGAALATM
                     TAGRQWGLPIIRPVSGIVTSPYGRRRILNGKPRSPHAGVDFRAAVGTPVLAALPGKVV
                     LTGDHYYAGKSIYIDSGGGVISHYFHMHTIDVKKGDPVKPGQVIGKSGVTGRATGPHL
                     HFGLALAGQMVNPEPLFSATSSGMLEKTALRKVTPRWK"
     gene            complement(1815..>2856)
                     /locus_tag="PK849_11245"
     CDS             complement(1815..>2856)
                     /locus_tag="PK849_11245"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPE66743.1"
                     /translation="LLVPLAAACGVKVAKLSGPGLSFTGGTVDKLEAIPGFQMHLSSE
                     RFIEQVRRIGCAISGHSDDLAPAEGKFYALRDVTGTVPTLPLLCSSIVSKKIAGGAHA
                     FVFDVKCGAGAFMEDYENAEKLARALVSLSKSLGKESMALITDMDQPLGEWIGNSTEV
                     LEAVSVLQGKGPKDVEELVINLAGAMVSIDSDLSFEEGCALAREKLEDGSGLEAFAAM
                     VRAQGGDDRVCRTPEKILPLAGKKHTITASEDSTVRALNARKIGEGVKRLGGGRMSLG
                     EPIDLSVGVRILKKNGASAGRGEALLEILYSDEAKLADAMPFFESAYTFGKANEHSED
                     TAQRRLILGKIG"
BASE COUNT          647 a          915 c          720 g          574 t
ORIGIN      
        1 agacggccta taccgcctcc caccggattt gaagtccggg gcgcccaccg ggacacattc
       61 gcctccatga accgttaaga attgtactgg cttccggtaa aaaagtccag tatcccggag
      121 cagcgcaaca atctttctat tggcttctcg cggaaaacca caccatatcg tgatccacca
      181 aaacctccct gacgagggga gctccgaaaa aatcccggaa ggagagcacg gacgctgcat
      241 tcgccatcgc ctttgccaca tcgagcgtgc tgattgccgg attgtcgttc gaggcgtcgt
      301 cgaagcgcac ccttgctacg ccgtctccgt cgatcacata agatcctgta aagcgcatca
      361 acgcccggac cttgaacgta agccgccgtt catacacgcc tttgatcatg acgctcccgt
      421 tgcgaagcgc cagttccgat acggcaaagt ggggcattcc ctcggagagc gttttcagaa
      481 aggtctcctt ggatatgccg ccagaaagat ggctctcacc gtgcgaatgc acaacaatgc
      541 cgcgtgccgc cgaagtaaag agaacatcat gcatcacgag cagcaacctg tcgtagcgaa
      601 cgccctcgac gacggcatcc tcgatgcaca ggttcaacgt cggaatcttg ccccgccgaa
      661 gctcatcctc tgaggcctta ataacggaca atactgattg cgcctggatc ctgtccgtga
      721 aatacttcgt aaaacgcgcc tcatagtccg ctataagaga actcgcccac ccagccgacg
      781 cgaaaaaaag caaaagcgcg cagctgccga aggctctctt ccaacctctc atccgcttcc
      841 ctcctcccgg cgaaagcata cagaaatccg acctcatccc cctgcatcac ttccagcgag
      901 gggttacttt ccggagcgcc gttttttcga gcatcccgga agacgttgcc gaaaaaaggg
      961 gttcaggatt caccatctgg cccgccaacg caagcccgaa atgcagatgc ggcccggttg
     1021 ccctgcccgt tacgccgctc ttcccgatca cctgccccgg cttgacggga tctccttttt
     1081 ttacgtcgat cgtgtgcata tgaaagtagt gagatataac cccgccgccc gaatcgatat
     1141 agatactctt cccggcgtag taatgatctc cggtgagcac gacctttccg ggaagcgccg
     1201 cgagaaccgg agtaccgact gccgccctga agtcaacccc cgcatgcgga ctcctcggtt
     1261 ttccgttcag tatgcgccgt cttccgtacg ggcttgttac aataccggat accggcctga
     1321 taatcggcag gccccattgc ctacctgccg tcattgtggc aagcgccgcg cccgtacgct
     1381 ttctttcggc ttctatccgc ttgagttccg aagaaggcgg agtcaccatc ttctcaggca
     1441 gcgtcaggcg gtcctccttg tactgaaccg gcattacctt gatcgagaaa ggatgttcag
     1501 aacgcttccc ccctgagagc agacggagcg tcagacgatg atctccggtc ttcactttgc
     1561 cgacctgcgt tccgagaagc gcgagcgaaa tgtaccgttc gttccacacg gaaactccga
     1621 gtcgcaccgt acgtccctgc caaacggcct ccactcccgt caacgggacg gaggaagtga
     1681 cgcgcactaa aaaaggcttc ccgacgccca ccgaggaggg gtactgaaca gtcacctcgg
     1741 ccgaagcgga cagcgggata caaacgaggc agaacaacag aaacagaatg ctgccgaaac
     1801 ggaaaaacgt aaatctaccc gatcttccca agaataagtc tcctttgcgc tgtatcttcg
     1861 ctatgttcgt tcgctttgcc gaacgtatag gcggattcaa aaaacggcat ggcgtctgcc
     1921 agtttcgcct cgtcggagta caaaatctca agcagcgctt ccccccgccc ggcggaggcg
     1981 ccgttcttct tcagaatccg cactcccacc gaaaggtcga taggttctcc cagcgacatg
     2041 cgccctccac cgaggcgctt gacgccttcg ccgatcttgc gggcgttcag ggcgcgcacc
     2101 gtggagtctt cagatgcagt gattgtatgt tttttgccgg caagcggaag gattttctcg
     2161 ggagtgcggc atacccggtc atcccctccc tgcgcgcgga ccatcgccgc gaatgcttca
     2221 aggcccgatc catcctccag cttctcccgt gcgagcgcgc acccctcttc gaaagagagg
     2281 tccgaatcta tggaaaccat cgctccggca aggttgataa cgagctcttc cacgtccttc
     2341 ggtcccttgc cctgaagaac ggaaacggct tcaagcacct ccgtgctgtt tcctatccac
     2401 tctcccaaag gctggtccat gtcggtaatc agcgccatgc tctcttttcc gagggatttg
     2461 gagagagaga caagcgcacg ggcgagcttc tcggcgtttt cataatcctc cataaaagcg
     2521 cccgccccgc acttcacatc aaagacgaag gcgtgagcgc ctccggctat cttcttgctc
     2581 acgatgctgc tgcaaagaag aggaagcgta ggcaccgttc ccgtcacatc acgcagggcg
     2641 tagaacttcc cctccgccgg agcaagatcg tcggaatgac cggaaatagc gcaaccgatc
     2701 cggcgcacct gttcgataaa acgctccgaa gagaggtgca tttgaaaacc ggggatggcc
     2761 tccagtttat cgactgtgcc gcctgtaaaa ctcagccccg gccctgaaag cttcgccacc
     2821 ttcacaccgc aggccgccgc cagaggaaca agaaga
//
LOCUS       DAOPKU010000395         2854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_39848_length_2854_cov_5.942122, whole genome shotgun sequence.
ACCESSION   DAOPKU010000395 DAOPKU010000000
VERSION     DAOPKU010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39848_length_2854_cov_5.942122"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK849_11250"
     CDS             <1..364
                     /locus_tag="PK849_11250"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049217.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPE66744.1"
                     /translation="GGLAAYTGTSDVREAKALIAEGDEAARLAYEAMAYSVAREIGAM
                     AAALSGKVDAILITGGVAYDNDFVALVQKRVQWIAPVLIYPGEDEMPALAEGAFRVLQ
                     GEELQKVYSEYISGGDRS"
     gene            361..1269
                     /locus_tag="PK849_11255"
     CDS             361..1269
                     /locus_tag="PK849_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPE66745.1"
                     /translation="MTMHGIDFLLAESKKGRSMTLALACPYGDDALGAACRAQREGIV
                     KTILIGDEGKIRTLAGRDGYDLSDIAIVDEKNDDTAVERSVKMVSSGEADLLMKGLVK
                     TSSLLKAVLDKEWGLRTGSLLSHLFLFEVPTLGGRVIGISDGGMNTYPDLNAKVKIVE
                     NAVACYHRIGVPQPKIAALAAVEVVNPDMPATLEAAALAKMNERGQIKGCLVDGPFAL
                     DNAVSEESARIKGISSPVAGNADMLLVPDIESGNFVGKVLLYMTGGKGAGVILGARKP
                     IVLTSRFDSMETKLLSIALGAVVARS"
     gene            1305..2234
                     /locus_tag="PK849_11260"
     CDS             1305..2234
                     /locus_tag="PK849_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPE66746.1"
                     /translation="MEQIRSLSQLLDYAKKIGAEKGPKKLSVAMAEEAGLLTAIEEAR
                     KAGFAEAILVGNGDKMKAAAEQAGVDLANYEVIDERDEPSMGISAVSQVSSKKADIYM
                     KGQIHTNNFLRAMLNKEVGLRKGKNTISHCYFHSVEGYDRIFFIADAAFNMYPDLAAK
                     ADITQNTVGLARSFGVAEPKVAVLAAVEVVNPDMPATLDAAALTQMNRRGQIKNCIVD
                     GPFALDNAVSEESAKTKGIVSPVAGKADIFIVPNIDAGNMLAKGIVYFSKNETAGLIL
                     GASAPIVLTSRADSTRAKLLSIAAAVTLSDFNK"
     gene            2248..>2854
                     /locus_tag="PK849_11265"
     CDS             2248..>2854
                     /locus_tag="PK849_11265"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPE66747.1"
                     /translation="MKILALNPGSTSTKVALFEDHTELWSETQRYDTDVIGRFSGVME
                     QEEFRLEEIRKCLAAHGATVADLDAVVGRGGLLKPIESGTYEVSDKMLEDLRACTWGA
                     HASNLGAPLAVRLADEGGCGKAYIVDPVVVDEMCPLARYSGLPEIERRSIFHALNQKA
                     VARRAAAELQKPYAECNLVVAHMGGGVSVGAHEKGRVIDVNN"
BASE COUNT          575 a          900 c          916 g          463 t
ORIGIN      
        1 gggcggcctc gccgcataca caggcacgag cgacgtgcgc gaggcgaagg cgctgatcgc
       61 ggagggggac gaggccgcga ggctcgcata cgaggctatg gcgtactcgg ttgcccgcga
      121 gatcggcgcg atggccgccg cgctctccgg aaaggtcgac gccattctca tcaccggcgg
      181 cgtggcgtac gacaacgatt tcgtcgcgct cgtacagaag cgcgtccagt ggatcgcccc
      241 cgtgcttatc tatccgggcg aggacgagat gcccgcgctc gcggaagggg cgttcagagt
      301 cctccagggc gaggagctgc agaaagtata ttccgagtac atctcaggag gcgatcgttc
      361 atgaccatgc acggtatcga ttttcttctt gcggagagta aaaaagggcg ctccatgacg
      421 cttgcgctcg cttgtcccta cggggacgac gcgttgggcg cggcgtgcag ggcgcagcgc
      481 gaagggatcg tcaagacgat cctcatcggc gacgagggga agatacgcac cctcgcgggg
      541 cgagacggct acgatctctc ggatatcgca atcgtcgacg agaaaaacga cgacacagcg
      601 gtggagcgga gcgtcaagat ggtctcctcc ggggaggccg acctgctgat gaaggggctc
      661 gtgaagacgt cgtcgctgct gaaggctgtg ctcgacaagg agtgggggct gcggaccggc
      721 tcgctgctct cccacctctt cctcttcgaa gtgcccacgc tcggaggccg ggtgatcggc
      781 atctccgacg gcggcatgaa cacctacccc gacctgaacg cgaaggtaaa gatcgtcgag
      841 aacgcggttg cctgctacca caggattggc gtcccgcagc cgaagatcgc cgcgctcgcc
      901 gccgtcgagg tggtgaaccc cgacatgccc gcaacgctcg aagccgccgc gctcgccaaa
      961 atgaacgagc gcgggcagat caaggggtgc ctggtcgacg gtccctttgc gctggataat
     1021 gcggtgagcg aggagtccgc gcgcatcaag ggaatctcct cgcccgtggc cggcaacgcc
     1081 gacatgctgc tggttcccga catcgagtcg ggcaacttcg tcggcaaggt gctgctctac
     1141 atgacgggcg gcaagggggc gggcgtcatc ctcggcgcgc gcaagcccat cgtcctcacc
     1201 agccgcttcg acagcatgga gacaaaactg ctttcaatcg ccctgggcgc ggtcgtcgcc
     1261 cggagctaaa aaagaatatt tcattcagag gaggagtttt tgttatggaa cagattcgct
     1321 cgctttccca gcttttggac tacgcaaaga agatcggcgc cgagaagggg ccgaagaagc
     1381 tctcggtcgc catggccgag gaggccggtc tgctgaccgc catcgaggag gcgcgcaagg
     1441 ccggattcgc cgaggcgatc ctcgtgggca acggcgacaa gatgaaggcc gcagcggagc
     1501 aggcaggagt cgacctcgcc aactacgagg tcatcgacga gcgcgacgag ccctcgatgg
     1561 ggatctccgc cgtctcccag gtttcttcca agaaggccga catctacatg aagggccaga
     1621 tccacacgaa caacttcctc cgcgcgatgc tcaacaagga agtcggactc cggaagggga
     1681 agaacaccat ctcccactgc tacttccaca gcgtcgaagg gtacgaccgc atcttcttca
     1741 tcgccgacgc cgccttcaac atgtacccgg atcttgccgc gaaggccgat atcacccaga
     1801 acaccgtcgg actcgcccgt tcgttcggcg ttgccgagcc caaggtcgcc gtcctcgccg
     1861 ccgtcgaagt ggtgaacccc gacatgcccg cgacccttga cgccgccgcg ctcacgcaga
     1921 tgaaccgccg cggccagata aagaactgca tcgtcgacgg cccgttcgcg ctcgataacg
     1981 cggtgagcga ggagtccgcc aagacgaagg ggatcgtctc ccccgtcgcc ggaaaggccg
     2041 acatcttcat cgtcccgaac atcgacgcgg ggaacatgct cgcgaagggg atcgtctact
     2101 tctcgaagaa cgagacggcc ggactcatcc tcggcgcctc cgcccccatc gtcctcacca
     2161 gccgcgccga ctcgacaagg gcgaagctgc tctccatcgc cgcggcggtc acgctctccg
     2221 actttaataa gtagagcgtt cgtcgttatg aaaatcctcg ctctcaatcc cggctccacc
     2281 agcaccaagg tcgctctctt cgaggaccat accgaacttt ggagcgaaac gcagcgctac
     2341 gatacggacg tgatcggccg tttctccggc gtcatggagc aggaggaatt ccgccttgaa
     2401 gagatccgca agtgccttgc cgcgcacggc gcaacggtcg cggacctcga cgcggtagtg
     2461 gggcggggag gacttctcaa gccgatcgag agcggcacgt acgaagtgag cgataagatg
     2521 ctcgaagacc tgcgcgcctg tacgtggggc gcgcacgcga gcaacctggg cgctccgctg
     2581 gctgttcgtc tggccgacga gggcggatgc gggaaggcgt acatcgtcga cccggtcgtc
     2641 gtggacgaaa tgtgtccgct tgcacgctac tccggacttc cggagatcga gcgccgctcc
     2701 atcttccacg cgctcaacca gaaggccgtg gccagaagag ccgccgcgga gctgcaaaaa
     2761 ccgtacgcgg agtgcaacct cgtcgtggcg cacatgggcg gcggcgtctc cgtgggcgcg
     2821 catgaaaagg ggcgcgtgat cgacgtgaac aacg
//
LOCUS       DAOPKU010000396         2841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_40139_length_2841_cov_3.962312, whole genome shotgun sequence.
ACCESSION   DAOPKU010000396 DAOPKU010000000
VERSION     DAOPKU010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40139_length_2841_cov_3.962312"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="PK849_11270"
     CDS             complement(<1..1651)
                     /locus_tag="PK849_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPE66748.1"
                     /translation="MSIKQRIGVFLALIILLAVPGLQAIAASAQETDVLALLEERQRA
                     VAELMEASTVYVLLDKGDSYSMGSAFIVAEGYAVTNAHVVGSAKGVLLMNSRLRPVKA
                     SVVRKEYDDETGGNDFALLRFSSSAKLPVLSFTLRVNRMDRVSAWGFPVLVTQFDQSF
                     DDILDGKRTKTPPVVYTEGTVSAVVERRGQKTVIHSAAIAGGNSGGPLVNARGEVVGI
                     NTWGYTEEDEGAFVNASIPSERIVDFLLKAGVKPQMSRQSPELARTPLPVPLPAPLPE
                     EPKKPAPSVELPAPPMEDDDGNEELFRMARAGDVDAQAALGAMYYDGDGVPQDTKQAV
                     QWLEKAVKGGSEEGKALLGVMLVFEEDVADPKKGVRLLREAASSVDEDGWISGLLGRI
                     YYEGEIYGVPRDLDESFRWAGKAAEGGDPDGTALLGMLYYFGEGTDEDDELALELTEQ
                     AVEEDSSLGKAILAWMYYDGVAVDEDLETALALATDAAEEEEALAQGLLALMYAEGHG
                     VERDVKSAEGWARRAADQGNEFGWFVLGSLYMRGEVVKKDPA"
     gene            complement(1783..>2841)
                     /locus_tag="PK849_11275"
     CDS             complement(1783..>2841)
                     /locus_tag="PK849_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HPE66749.1"
                     /translation="IMMKNLRDMQAYADRHGVALRPHTKTHKAPAIARMQVEGGAKGI
                     AVAKVGEAETMAANGLNDIFIANEIVGVRKLERIRALAERIDISFGVDSPCQAEAAEA
                     VFAQADRPAQLLVEIEVGENRSGIIEEEDFVRLLQTIRSCPHVEFKGLFSHDGNSYAA
                     SSQEECRRISVGAQQRTLRFAAIAREMGMPPRTVSIGSTPSLMNESEIDRGVTEIRPG
                     TYILMDASQGNAVGTLERCAATVLATVISRPTAERVILDVGAKGLTMQGRSEGICATP
                     GKGTLPDFPGVYIHAVYDEHAIIYNKEFRETVRIGDKVRIIPVHICPVCNLYDSAFLI
                     SGDEVVGEIPILCRGKLQ"
BASE COUNT          451 a         1028 c          825 g          537 t
ORIGIN      
        1 tggcggggtc cttcttcaca acctcgccgc gcatgtacag ggatcccagg acgaaccagc
       61 cgaactcgtt cccctggtcc gccgccctcc gcgcccaccc ttcggcgctc ttcacatcgc
      121 gctccacccc atgtccctcg gcgtacatca gcgcgaggag cccctgcgcc agagcctctt
      181 cctcctcggc cgcgtccgtc gccagcgcca gggccgtttc aagatcctcg tcgaccgcga
      241 cgccgtcgta gtacatccac gcgaggatcg cctttccgag cgagctgtcc tcctcgaccg
      301 cctgctccgt cagctccaga gcgagctcgt cgtcctcgtc cgtcccttcg ccgaagtagt
      361 agagcatccc cagcagcgct gtgccgtccg ggtctcctcc ctcggcggcc tttccggccc
      421 accggaaact ctcgtcgagg tcccggggca cgccgtagat ctccccctcg tagtaaatgc
      481 gcccgagcag tccggaaatc cagccgtcct cgtccacgga agaggccgct tcgcggagca
      541 ggcggacgcc cttcttcggg tcggcgacat cctcctcgaa gacgagcatg acgccgagca
      601 gcgcttttcc ctcctcgctc ccgcccttta ccgccttctc cagccactgg acggcctgct
      661 tcgtgtcctg cggcacgccg tctccgtcgt agtacatcgc ccccagggcg gcctgcgcgt
      721 cgacgtctcc cgccctcgcc atacggaaca actcctcgtt cccgtcgtcg tcttccatcg
      781 ggggcgcggg gagctcgacg gacggggcgg gcttcttcgg ttcctctgga agaggtgcgg
      841 gaagcggcac aggaagcggc gtccgggcga gttccggcga ctggcgcgac atctgcggtt
      901 tgacgcccgc tttcagcagg aagtcgacga tccgctccga cggtatggag gcgttcacga
      961 acgcgccttc gtcctcctcc gtataccccc atgtattgat cccgacgacc tcgccgcgcg
     1021 cgttcaccag agggccgccg ctgtttcccc ctgcgatggc cgcggagtgg atgacggttt
     1081 tctggcctct ccgctcgacc actgcgctca ccgtgccctc ggtgtacacc accggagggg
     1141 ttttggtccg ctttccgtcg agaatatcgt cgaagctctg gtcgaattgg gtgacgagca
     1201 ccgggaaccc ccacgcgctc accctgtcca tcctgttcac ccgcagcgta aaggagagca
     1261 caggcagctt cgcagaggag gagaatcgca gcagggcgaa gtcgttccct cccgtctcgt
     1321 cgtcatactc cttccgaacg acggaggcct tcacggggcg cagccgggag ttcatcagca
     1381 gcaccccttt cgccgacccc accacgtgcg cgttggtcac cgcgtagcct tccgccacga
     1441 tgaaggcgct ccccatgctg taggagtccc ccttgtcgag cagaacgtag acggtggacg
     1501 cctccatgag ctccgccacg gcccgctgcc gttcctcaag gagagcgagc acgtccgtct
     1561 cctgcgcgga ggcggcgatc gcctgaagcc ccggtacggc gagaagaatg ataagagcaa
     1621 gaaacactcc gatacgttgt ttgatcgaca cagtgcactc caccttctca aggatagtgt
     1681 ttttcgataa ctctttcttt tcagatagta ccttgccgaa cttccgatgt caacaaaagg
     1741 gggcgaacga cgccgacggg ccgaaagccg gtgcggcagg cgttactgca actttcccct
     1801 gcacagaatc ggaatttccc ccaccacttc atcgcctgag atcaggaacg cgctgtcgta
     1861 caggttgcat accggacaga tatgcacggg gatgatccgc accttgtcgc cgatccggac
     1921 ggtttcgcgg aactctttgt tgtagatgat cgcgtgctcg tcgtagaccg catggatata
     1981 cacgcccgga aaatccggga gcgtgccctt tcccggcgtc gcgcagatcc cctcgctccg
     2041 cccctgcatc gtcagccctt tggcccccac gtccaggatc acccgctccg ctgtagggcg
     2101 gctgatgaca gtcgccagca ccgtcgccgc gcagcgctcc agcgtgccga cggcgttccc
     2161 ctgcgacgcg tccatcagga tgtacgttcc cggacggatc tccgtaacgc ccctatcgat
     2221 ctccgattcg ttcatcagcg aaggggtgga gccgatgctc accgtccgcg gcggcatccc
     2281 catctcccgc gcgatcgccg cgaagcggag ggtgcgctgc tgcgccccga cggagattcg
     2341 cctgcactcc tcctgcgagg aggccgcgta gctgttgccg tcgtgggaga agagcccctt
     2401 gaactccacg tgcggacagg agcggatcgt ctgcagcagg cggacgaagt cctcctcctc
     2461 gatgatgccg gagcggttct cgcccacctc gatctccacc aggagctgcg cgggacggtc
     2521 ggcctgcgcg aagacggcct ccgccgcctc cgcctggcac ggggagtcca cgccgaagga
     2581 gatgtcgatc cgctccgcca gcgcgcgaat ccgctccagc ttgcggacgc cgacgatctc
     2641 gttggctatg aagatatcgt tcaggccgtt cgcggccatc gtctccgcct cgccgacctt
     2701 ggccaccgcg atgcctttcg cgccgccctc cacctgcatc cgcgcgatcg cgggcgcctt
     2761 gtgcgtcttc gtgtgcgggc gcagcgcgac tccgtgccgg tcggcgtacg cctgcatgtc
     2821 ccgcaggttt ttcatcataa t
//
LOCUS       DAOPKU010000397         2838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_40189_length_2838_cov_4.450952, whole genome shotgun sequence.
ACCESSION   DAOPKU010000397 DAOPKU010000000
VERSION     DAOPKU010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40189_length_2838_cov_4.450952"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1207)
                     /locus_tag="PK849_11280"
     CDS             complement(23..1207)
                     /locus_tag="PK849_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE66750.1"
                     /translation="MRWIGWVLLCGCFHMAYVLRLSAGVLREPLSAEFTLSASSFGLM
                     SSMVFYAYTLLQIPSGVLADTRGVRSTVAGGMALAGAGALLFASARSLPALFFGRALM
                     GVGVAGIFVCVMKFLAENFERERFATLSGATSFAGNAGGIVAQAPLAMLVGALAWRGA
                     FLSLGAVCLALAVLSALFLPAGRRSDVSQAELRAGVRAILRTRGMYFTGLNYLANQSC
                     FLALSGTWGVSYLRAVHGIDQAPSYITLMVVGVMAGAVVAGRISDGARSRRRPLRLFA
                     AAHALCWGALLLSGSGISLYALAGTLFSLGFFAGALVLPWSMAKEMNPPEFTGVAIAL
                     LNTAAFLAVAVLTSLMGVALDAAAGLSAEAAWRAALLLPFAVSVLGLWGAFASPETFP
                     DA"
     gene            1429..1824
                     /locus_tag="PK849_11285"
     CDS             1429..1824
                     /locus_tag="PK849_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HPE66751.1"
                     /translation="MAKQAKQPRIETEPDAAPLALSLGALLGREEVVRAPEDVPHEEE
                     KAPKPPRELPVRVILSRETKGRGGKTVTRLSFREGTPSDPEGLAKKLRTAMGCGGTVE
                     GDDIVLQGDQIQRAAEWFAARKVRVTGAR"
     gene            1951..>2838
                     /locus_tag="PK849_11290"
     CDS             1951..>2838
                     /locus_tag="PK849_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE66752.1"
                     /translation="MISSRKDRLYGVFLVLMASMCWGTTGTLQALAPEGAPPLTVGSI
                     RITLSALILLPWCVWRDGGIGFLRRTSLPALLVGVAGLVGFQFSFFTALKLTGVSIGT
                     MIAIGASPMFAGALGSLVQREPLSVRWFISTAVAVFGCALLVLGGSSGPMLLNPLGIS
                     LAFAAAFFYALMGLGFKMQGATLNDTQTIAVTTGTALIVALPVLLSLDSSWIFTRAGL
                     GISFSLGFATMALPMSLFSLGLRKIYLRDAYTISLAEPLTACILSALVLGERLSPLSI
                     GGAALIFCGILLLPVASEGG"
BASE COUNT          549 a          954 c          881 g          454 t
ORIGIN      
        1 aatcacctct tcatagacgt cgtcatgcgt cgggaaaggt ctcgggggac gcgaacgctc
       61 cccaaagccc caggacggag acggcgaagg gaagcagcag cgcggcccgc catgccgctt
      121 cggcggagag tccggccgcc gcgtcgagcg cgacgcccat cagggatgtc agcaccgcca
      181 ctgcgaggaa ggccgccgta ttcagcagcg cgatggccac gcctgtgaac tccggcgggt
      241 tcatctcctt ggccatggac cagggcagca ccagcgctcc ggcgaaaaac cccagcgaga
      301 aaagcgttcc tgcgagcgca tacagagaaa tcccgcttcc ggagagcaga agcgcccccc
      361 agcagagtgc gtgcgccgcc gcgaagaggc gcagaggacg ccgcctgctc cttgcgccgt
      421 cggaaatccg ccctgcaacg accgcacccg ccatgacccc gacgaccatg agcgtgatgt
      481 acgaaggggc ctggtcgatc ccgtgcaccg cacgcagata cgacacgccc cacgtaccgg
      541 agagggcgag aaaacagctc tgatttgcga gatagttcag tccggtgaag tacatccccc
      601 gagtccggag aatggctcgc acacccgcgc ggagctcagc ctgcgaaacg tcgcttctgc
      661 gccccgctgg gagaaaaagt gcggagagta ctgcgagggc gaggcagacc gcgccgagcg
      721 acaggaacgc gcctctccat gccagcgctc ccacgagcat cgccagagga gcctgcgcaa
      781 cgatcccgcc cgcgttccct gcgaacgacg tcgctcccga gagcgtggcg aagcgctccc
      841 tctcgaagtt ttcggcgagg aacttcatca cgcagacaaa aatccccgcc actccgacgc
      901 ccataagcgc ccttccgaaa aagagcgcgg gcagcgacct tgcggacgcg aaaagcagcg
      961 ctccggcccc ggccagagcc atcccgcccg cgacggttga gcggacgccc cgcgtatcgg
     1021 ccagcacgcc cgacggaatc tgcagcagcg tgtaggcgta gaagaccatc gaggacatca
     1081 ggccgaagga ggacgccgaa agcgtaaact cagcagaaag cggctcccgc agcactcccg
     1141 ccgagaggcg cagaacgtac gccatgtgaa aacagccgca gagtaacacc catccgatcc
     1201 aacgcatact aaaaattctc cttccatcgt tcagcgaagc cagggggaac tgcaaaacac
     1261 tcgcaacgac aagagaaacc gagatatcgc tcctcaggca aaacgcgacc ttcgaagaga
     1321 aactcagacc ctcgctctgg ttttttcaac cgccctattc tagaataaat cccgtctgaa
     1381 agatacgcct ccggcggatg tccggagctt acggaagagg ggaagaacat ggcaaaacag
     1441 gcgaaacagc cgcgcatcga aaccgagccg gacgccgcgc cgctggcgct ctcgctgggg
     1501 gcgcttctgg ggagggagga ggtcgttcgg gcgcccgaag acgttccgca cgaggaggag
     1561 aaggccccca agccgccgag ggagcttccc gtacgggtga ttctttcgcg cgagacgaag
     1621 gggcgcgggg gcaagaccgt cacgcggctt tcgttcagag agggaacgcc ctccgatccc
     1681 gaggggcttg cgaaaaaact ccgcaccgcg atggggtgcg gaggaacggt ggagggagac
     1741 gacatcgtgc tccagggcga ccagatccag cgggccgcag agtggttcgc agcccgcaag
     1801 gttcgggtga ccggagcgcg ctgacggatt tgggcggatg aaaatcattt gacgaaacca
     1861 cccgttcgca ttagcattag tcacgcccgg cgcacgccgg gaacattcgg actttccgaa
     1921 aatcgacgcg agcgagagtg actgttaccc atgatatcgt cccgaaaaga taggttgtac
     1981 ggcgtattcc ttgttcttat ggcgagcatg tgctggggaa ccaccggcac gttgcaggcg
     2041 ctggcccccg agggggcgcc ccccctcacc gtcggctcga tccgcatcac gctctccgcg
     2101 ctgatcctgc tgccctggtg cgtctggcgg gacggcggaa tcggcttttt gcgcaggacg
     2161 tcgctccccg cccttctggt gggcgtcgcg gggcttgtgg gctttcagtt ttccttcttc
     2221 accgcgctca aactgacggg cgtctccatc ggcacgatga tcgccatcgg cgcgtcgccc
     2281 atgttcgccg gggcgctcgg ttcgctggtg cagcgcgagc ccctttccgt ccgctggttc
     2341 atctcgacgg cggtcgccgt ctttggctgc gcgctgctgg tcctcggcgg ctcctccggc
     2401 ccgatgctgc tcaatccgct gggaatctcc ctcgccttcg ccgccgcgtt cttttacgcg
     2461 ctgatggggc tggggttcaa gatgcagggg gcgacgctga acgacaccca gacgatcgcc
     2521 gtcaccacag gaaccgccct gatcgtcgcc ctcccggtgc ttctctcgct ggactcttcg
     2581 tggatcttca cccgcgccgg gctggggatc tccttttctc tcggcttcgc caccatggcc
     2641 ctgccgatga gcctcttctc gctggggctg cggaagatct acctccggga cgcctacacg
     2701 atctccctcg ccgaaccgct caccgcgtgc atcctctccg cgctggtgct cggggaacgg
     2761 ctctcccccc tctccatcgg gggcgcggcc ctgatcttct gcggcatcct gctgcttccc
     2821 gtcgcgtcgg agggcggg
//
LOCUS       DAOPKU010000398         2838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_40190_length_2838_cov_4.428315, whole genome shotgun sequence.
ACCESSION   DAOPKU010000398 DAOPKU010000000
VERSION     DAOPKU010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40190_length_2838_cov_4.428315"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PK849_11295"
     CDS             <1..687
                     /locus_tag="PK849_11295"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPE66753.1"
                     /translation="YFGYARQDRKTKPRDPISAKLMANLLTEAGATRVISADLHAGQI
                     QGFFDIPVDNLTGIPLLASYFKEILAECVECGSLTVVAPDVGGVVRARKFAGTLKADL
                     AIVDKRRSHEVANLCEVMDVIGDIRGKTAILVDDIIDTAGTICNAAGLLKERGAVKIY
                     ACSTHGVLSGPALDRLEHSDIDEVVLTDTIPLPESKKSAKIVQLSIAPLFAEAILRVH
                     SDRSVSSLFD"
     gene            755..1408
                     /locus_tag="PK849_11300"
     CDS             755..1408
                     /locus_tag="PK849_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPE66754.1"
                     /translation="MSEMISIVLEERKEIGKEVCKKMRPAGYTPCVFYGPEYVESVPA
                     KVKTAGIAKLLKSGQWETVTFDATLPDGRKEMCLIREVQKDFLNGNVLHIDFMQIIKG
                     HKISVNVPLHITGRDACKGVKLGGVIDQILREISMEVLPGQIPDSFTVDISSLGLGEQ
                     IHVRDLALPSDATLLVDKDDVAVAIIIPRGVAEAEAEEEQKEVEVVAKGKAKSADEE"
     gene            1415..1981
                     /gene="pth"
                     /locus_tag="PK849_11305"
     CDS             1415..1981
                     /gene="pth"
                     /locus_tag="PK849_11305"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPE66755.1"
                     /translation="MKIVAGLGNPGPEYVFSRHNAGWLAVDHLVNRFSCGTPRMQFSS
                     MAWSFFMNGEKILILKPLTYMNLSGKAVGEAVDYLSADWEDVLVVYDDVALPFGKLRM
                     RSKGSAGGHKGLLSILGRAGTLEVPRLRIGVGAAPDARGIVSWVLGNFDKTEQNELPS
                     LLDSTAEAVEEWLSVGTEKAMNRVNATH"
     gene            2058..>2838
                     /locus_tag="PK849_11310"
     CDS             2058..>2838
                     /locus_tag="PK849_11310"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPE66756.1"
                     /translation="MKTPVETRKNRVWDVEEALWKQGVSIHLPVDGAARCWVCRAPQG
                     SVVAVFPDAKQAGTFLADWKALFPEEPVGYLQEIPLTPQGISNKALAVQRGETLSRWR
                     EEGGVLVATGGGLLGPVARGGGEILLEVEKEYERNALLDWLVHSGYVRSDLVWAPGQF
                     VLRGYILDVYDPAYAYPLRLEFYDDTLESIRSFSPRTQKSVAMLDALHIHSLSITREA
                     SVLDFFTETRPGASTESAGEPFFLFFEPVKIESSAETYELLW"
BASE COUNT          608 a          793 c          862 g          575 t
ORIGIN      
        1 tacttcggct acgcgcggca ggacagaaag acaaagccca gggatcccat ctccgcgaag
       61 ctcatggcga acctgcttac cgaggcgggg gccacgcgtg ttatttcggc ggatcttcac
      121 gccgggcaga tacagggatt tttcgatatc cctgtggata acctgacggg gattcccctg
      181 ctcgcgagct acttcaagga gatcctggcg gagtgcgtcg aatgcggttc cctcacagta
      241 gtggcgccgg acgtgggcgg cgtggtccgt gcaaggaagt tcgccggaac gctgaaggcg
      301 gatctcgcca tcgtcgacaa gaggcgttcg cacgaggtcg cgaacctgtg cgaagttatg
      361 gacgtcatcg gcgatatccg cgggaagacg gcgatcctgg tcgacgatat catcgacact
      421 gcggggacca tctgcaatgc cgcggggctg ctcaaggagc ggggcgccgt gaagatctac
      481 gcctgctcga cgcacggcgt tctttcgggg ccggctctcg accgtctgga gcactccgat
      541 atcgacgaag ttgtgttgac ggatacaatc ccattgcctg agagcaaaaa atcagctaaa
      601 attgttcagt tgtcaatcgc acccctattt gccgaggcta ttctcagagt acacagcgac
      661 cgctcggtga gcagtctttt cgattaactg cggggagatg tcaagttttc cgattccgta
      721 gaaggagtcg attttcaagg aggatgtgtt gggcatgtcg gaaatgatca gtatcgtact
      781 ggaagagcgc aaggaaattg gaaaagaagt ctgcaagaaa atgcgccccg cggggtacac
      841 tccctgcgta ttctacgggc cggagtatgt ggaatccgtt cccgcgaagg tgaaaacagc
      901 cgggatcgcc aaactgctga agtccgggca gtgggagacg gtgactttcg acgcgaccct
      961 gccggacggc aggaaggaga tgtgccttat acgggaggtc cagaaggact tcctgaacgg
     1021 caacgttttg cacatcgact ttatgcagat catcaagggg cacaaaatca gcgtcaacgt
     1081 tcctctccat atcacggggc gcgacgcatg caagggcgtc aaactcggcg gggttatcga
     1141 tcagatcctt cgtgaaatct ccatggaagt tctccccggc cagattcccg attccttcac
     1201 cgtggacatc tcctccctcg ggctcggcga acagatccat gtgcgcgacc ttgcacttcc
     1261 ctccgacgct acgctgcttg tcgacaagga tgatgtggcg gttgccatca ttattcctcg
     1321 cggcgtcgcc gaggccgaag cggaagaaga gcagaaggaa gtcgaagtcg tcgccaaggg
     1381 caaggcgaag agcgcagacg aggaataacg ccggttgaaa atcgttgctg gtttgggaaa
     1441 tcccgggccg gagtacgtct tctcccgtca taacgcgggg tggctcgcgg tggaccatct
     1501 ggtgaaccgt ttttcctgcg gaacgccgag gatgcagttc tcttcgatgg catggtcgtt
     1561 ttttatgaac ggggagaaga tcctgattct caaaccgctc acctacatga acctcagcgg
     1621 gaaagcggtc ggagaggctg tggactacct ttcagccgat tgggaggacg tccttgtagt
     1681 gtatgacgac gtcgcgctcc ccttcgggaa gctccggatg cgctccaaag gaagcgctgg
     1741 aggacacaaa gggctgctct ccattctcgg ccgggcggga acgctcgaag tgcccaggct
     1801 gcgcatcgga gtcggcgccg cgccggatgc ccgcgggatt gtctcctggg tgctcggcaa
     1861 cttcgataaa acagaacaga acgaacttcc ctctcttctc gacagcactg cggaggccgt
     1921 tgaagaatgg ctctccgttg gaaccgagaa ggcgatgaac cgggtgaacg caactcattg
     1981 acaaagaggt ggcggagaag tccgccacct ctttgcgcac agtctcccgg agcggagcgg
     2041 attccggagt cgaacgtatg aaaacccctg tagaaacaag aaaaaaccga gtatgggacg
     2101 ttgaggaagc gctgtggaag caaggcgtct ccattcatct gcctgtcgac ggcgccgcca
     2161 gatgctgggt ctgtcgggcg ccgcaagggt ctgttgtcgc tgtgtttccg gatgcgaaac
     2221 aggccggaac atttctggcc gactggaaag ccctctttcc ggaggagcct gtcggctatc
     2281 tccaggaaat ccccctcacg ccccagggaa tctcgaacaa ggcgctcgcc gtacaaagag
     2341 gtgagacgct ctcccgctgg agagaggagg ggggcgttct cgtcgctaca ggcggaggac
     2401 ttcttggccc cgtcgcgcgg ggcggcggcg agattcttct cgaagtggaa aaagagtacg
     2461 aacggaacgc ccttctcgac tggctggtgc attcagggta tgtccggtcc gacctcgtct
     2521 gggcgcccgg acaattcgta ctccgcgggt atatcctcga cgtctacgac cctgcctacg
     2581 catatccgct gcggcttgag ttctacgacg ataccctgga gagcatccgc agcttctcgc
     2641 cgcgaacaca aaagagcgtc gccatgctcg acgctctcca tattcattcg ctctctatca
     2701 cgcgcgaagc atccgtgctt gattttttca cggaaactcg ccctggcgcg tcgaccgaat
     2761 ccgccggcga gccgttcttt ctcttcttcg agccggtcaa aatcgaatcg tccgccgaga
     2821 cctacgagct cctatgga
//
LOCUS       DAOPKU010000399         2832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_40315_length_2832_cov_3.881527, whole genome shotgun sequence.
ACCESSION   DAOPKU010000399 DAOPKU010000000
VERSION     DAOPKU010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40315_length_2832_cov_3.881527"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /gene="flgN"
                     /locus_tag="PK849_11315"
     CDS             complement(<1..398)
                     /gene="flgN"
                     /locus_tag="PK849_11315"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HPE66757.1"
                     /translation="MPSPSSLGDALAKQAEILRTLGEVTVRQREALKEGRLELLQDLF
                     REQQAAGFAAEAAEERRRRAASELAAALGCESNVSAICSRLPEQAALLLKKEADEVLR
                     QVKVLQSEMHIISALVEENQRLNGMMAAEWR"
     gene            complement(401..649)
                     /gene="flgM"
                     /locus_tag="PK849_11320"
     CDS             complement(401..649)
                     /gene="flgM"
                     /locus_tag="PK849_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPE66758.1"
                     /translation="MDKLNAKKGYKPSREGGKGDGVEFSSFAVALAKVSGELKKIPDV
                     REQLVEDFKKQIAEGTYSPDMNQVARSLIGAGLLDREE"
     gene            complement(726..1376)
                     /locus_tag="PK849_11325"
     CDS             complement(726..1376)
                     /locus_tag="PK849_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPE66759.1"
                     /translation="MSTLEELFRFAAHLFWPRACPFCGALGESACVLCLLPCLEPPMP
                     MELGSLPLWSGGEHDGVLRELVLALKYGGDRSLGVAMGRALGRIFPRPDADLLVPVPL
                     HRGSERAYNQSLALAAGAAKEWGIPAKEGLVWSERREEQTSLGQEERKMMPRDVFSPV
                     TKSVAGKRIVLVDDVATTGTTLERAAETVRRGGGDPVLALSWTCAPRTKEGMRGVR"
     gene            complement(1363..1782)
                     /locus_tag="PK849_11330"
     CDS             complement(1363..1782)
                     /locus_tag="PK849_11330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPE66760.1"
                     /translation="MNGGYFFYDSKNAVLASELANLYRFTRWGRSRSVEDIDRMLRGT
                     SMCFSVRYEEHLVAFCRILTDFIYRGSLWDIMVHPDHQGKKVGTALLDYALNHPAIKP
                     IPLIITYTSELAPFLVPHGFAPKEGALMLLRRPIEYS"
     gene            complement(1779..1997)
                     /locus_tag="PK849_11335"
     CDS             complement(1779..1997)
                     /locus_tag="PK849_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HPE66761.1"
                     /translation="MLIVACQICGETKVIAGTPDGDGMARATWICPVCGTGQVMQLPV
                     SSDARKSDLRSIVCGMALATHGGMEERP"
     gene            complement(2019..>2832)
                     /locus_tag="PK849_11340"
     CDS             complement(2019..>2832)
                     /locus_tag="PK849_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66762.1"
                     /translation="LQEQQKKLAGVQAELESVAPLLDEARASLVRTQTEKEQVEQEKA
                     ALSRSVEELKEEAEALRKGLIQVRSGRISVFAMEILAQAPVEPMSSRSDVERIFQELR
                     KQAEFVVAARTGQPSADVLLSVDLEKEAERIAECSGQPSRKFVRAIAEGNAVFGEDIR
                     VVYEVYDSVLVYRKGELLTRRTLKPYEGINAETELHLILRMVNRKAVADDIKPDPVTG
                     NVGTLDGAQFFEAVDRLTRADGPVLVEVTAAEDIHTEGPVRVSIEIRPAEEN"
BASE COUNT          510 a          880 c          843 g          599 t
ORIGIN      
        1 cgccactccg cggccatcat accgttgagg cgctggttct cctcgacgag cgcggagatg
       61 atatgcattt ccgactgaag cacctttact tggcggagaa cttcgtcggc ttcttttttc
      121 agcaggaggg ccgcttgttc agggagtcgc gaacaaatgg ccgatacgtt ggattcgcac
      181 ccaagcgcgg ctgccagctc ggaagcggcg cgtctgcgcc gctcttcggc ggcttccgca
      241 gcgaacccag ccgcctgttg ttcgcggaag aggtcctgta ataattcgag gcgcccttct
      301 ttgagcgcct ctctctgccg tacagtaact tcaccgagcg tgcgcagaat ctccgcctgc
      361 ttggccagcg cgtctcccag agaggacggt gaaggcacgt ctactcctcc cggtcgagca
      421 gccccgcgcc gataaggctt cgcgccactt ggttcatatc cggtgagtag gttccctcgg
      481 ctatctgttt tttgaaatcc tcgacgagct gttcgcgaac gtcgggtatc ttttttaact
      541 cgccggaaac tttagcgagc gcgacggcaa aggagctgaa ctctactccg tcgcctttgc
      601 ccccctcgcg ggaaggtttg taccctttct tcgcgttaag tttgtccatt acgtttggtc
      661 catacacgcc gggaatctta tcgatcattt ttatcacccc gaaagaaact tttctcttca
      721 ctttgttatc ggacgccgcg catcccctct ttagtccggg gcgcgcaggt ccacgaaagt
      781 gcaaggacgg gatcgccccc gccgcgacgt actgtttcag cggctcgttc caaggttgtg
      841 ccggttgtgg cgacgtcgtc cacaaggacg attctctttc ccgcaacgct cttcgtcaca
      901 ggcgaaaaga cgtcgcgggg catcattttc cgttcctcct gccccagaga ggtctgttct
      961 tcacggcgtt cgctccagac gagcccttct ttggcaggaa tcccccattc ttttgccgcg
     1021 cctgcggcaa gagcaagaga ctggttatag gctcgttcgc tcccccggtg cagcgggacg
     1081 ggaacgagca ggtcggcgtc cggacggggg aagatgcgcc ccagcgcacg gcccatagcg
     1141 acgccgagcg accggtctcc tccgtacttc agcgcgagga caagctcccg gagcacgccg
     1201 tcgtgctctc cgcccgacca gagcgggagg ctcccaagct ccatcggcat cggaggctca
     1261 agacagggca acagacagag aacgcacgcg gactcgccga gcgcgccgca gaaggggcac
     1321 gctctgggcc agaaaaggtg cgcggcaaac cggaaaagct cctcaagagt actcaatcgg
     1381 ccgcctcagg agcatgaggg cgccctcttt cggggcgaag ccgtgcggaa cgagaaaggg
     1441 ggcgagttcg ctggtatagg tgatgatcag cggaatcggc ttgatcgccg ggtggttgag
     1501 cgcatagtcg agcagcgcgg ttcccacctt tttgccctga tgatcagggt gtaccatgat
     1561 gtcccagaga gatccccggt agataaagtc ggtcaatatc ctgcagaaag cgacaaggtg
     1621 ctcctcgtaa cgcacgctga agcacatcga ggtgccgcga agcatgcggt cgatgtcctc
     1681 aacggagcgg cttcgccccc atcgggtaaa gcggtagagg ttggccagct cggaggcgag
     1741 caccgcgttc ttgctgtcgt aaaaaaagta gccgccgttc acggcctttc ctccatacct
     1801 ccgtgcgtcg caagggccat cccgcagacg atggagcgga gatcggattt ccgggcgtcg
     1861 gaggagaccg gaagctgcat gacctgcccc gtgccgcaga cgggacagat ccacgtggcg
     1921 cgggccattc cgtcgccgtc gggcgttccg gcgatgacct tcgtttctcc gcaaatttga
     1981 caggcgacta tgagcattgc gcattcctcc acagggaact aattttcttc ggcagggcgt
     2041 atctcgatgg agacgcgtac cggaccttcc gtatgaatgt cctcggccgc ggttacctcg
     2101 acaagcaccg gtccgtccgc ccttgtgaga cggtccacgg cttcaaagaa ctgcgccccg
     2161 tcgagggttc ccacattgcc ggtgacggga tcgggtttga tgtcgtcggc aacggctttt
     2221 ctgttcacca tccggaggat gaggtgcagc tcggtctccg cgttgattcc ttcgtagggt
     2281 ttgagcgtcc gccgggtcaa aagctctccc tttctataga cgaggacgct gtcgtacact
     2341 tcgtagacga cgcggatatc ctccccgaaa accgcatttc cctcagctat cgcgcggacg
     2401 aacttcctcg aaggctgacc ggaacactcg gcgatacgct ccgcctcttt ttccagatcg
     2461 acggaaagga gcacgtcggc ggacggctgg ccggttctgg cggcaacgac gaactccgcc
     2521 tgtttacgca gctcctggaa gatgcgttcg acgtcgctgc ggctgctcat cggctcgacg
     2581 ggcgcctgag cgagtatttc catggcgaag acggagatgc ggccgctgcg tacctggatg
     2641 agccccttcc tcagggcttc cgcttcctcc ttgagctctt cgacggagcg tgagagcgcg
     2701 gccttctcct gctccacctg ctctttttcc gtttgagtgc ggacaaggct tgcgcgggcc
     2761 tcgtcgagca ggggcgcgac gctctccagc tccgcctgga ctccggcgag cttcttctgc
     2821 tgctcttgga gt
//
LOCUS       DAOPKU010000400         2831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_40334_length_2831_cov_4.862752, whole genome shotgun sequence.
ACCESSION   DAOPKU010000400 DAOPKU010000000
VERSION     DAOPKU010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40334_length_2831_cov_4.862752"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1031
                     /locus_tag="PK849_11345"
     CDS             282..1031
                     /locus_tag="PK849_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPE66763.1"
                     /translation="MEPIIPFQRKNRSDMIHEKLHELIREGHWKPGDKFPTEMELAKL
                     FNAGRSTVREALNLLKASNLLKVIPGHGTVVASPSELEHNVLSRHIPDSQTARELFNI
                     VEFRICLEPSTTYLAAERIVPAQSDRLLELAGAVNDDTLTPSGFAETDIAFHAFLAEI
                     AGNPLCIETMRSLHNFFYKQQIVTSLISTRKKPVAQSHIAIAKAVADHNAKLAEKEMR
                     AHLYNTRQYLESIVRETHVPKNFLKTSGGES"
     gene            1052..1849
                     /locus_tag="PK849_11350"
     CDS             1052..1849
                     /locus_tag="PK849_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPE66764.1"
                     /translation="MKEKARAVRLGIIDALGVDKRGHLGGSMSCADIVAALYFHKMRH
                     DPKNRFMADRDRFIMSKGHSVLAQYAALAEAGYFPRETLLKTKTLGSILQGHPERETP
                     GVEANTGSLGQGLSIGVGMALAGRLDKLNYRTWVVLGDGELAEGQVWEAVTAAAAFKL
                     DNLTAIVDMNGIQAVGRTCDRYNLGNIRGKFDAFGWKTFEIDGHDMAQVLQALDDASE
                     VRGAPSAIIAGTVKGKGVSFAENTPAYHNNLLTKEQQEAARREIMAM"
     gene            1864..2793
                     /locus_tag="PK849_11355"
     CDS             1864..2793
                     /locus_tag="PK849_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPE66765.1"
                     /translation="MLMNLRAAYGEALLALAGENKNVVALDADLCGSTQSVVVEKNYP
                     ERYFEMGIGEQNMISVAAGLALTGKIPFAHSFSVFATGRPFDQIRQSVCLPNLNVKIV
                     GSSCGLSDFGDGATHQSFEDLATMRVLPHMTVLCPADGIEVKWAVHAAAAIDGPVYIR
                     LNRNDLPVLFDEKTSFEIGKPLVVKEGNDVALVCTGTMTSIALDAAALLEQQGISARV
                     VHLGTIKPLCGKSLREALGNVRGIVSCEEHTIYGGLGSAVAEALCEAPLPMKMVGIQD
                     RYGQSAVCYEELLECYGLTKEAVAKAAEALLKK"
BASE COUNT          639 a          865 c          834 g          493 t
ORIGIN      
        1 aacggagacc gctgaaaaaa tgcactccct tctgaacgcg taaaaatcgc atgggttttg
       61 ctcactgagc gattttcttc cgattattgt acgcacccct ccctctcctt acggtcaata
      121 cgaaaagaca ggcgcaaggg atgagtcgcc cacatctcca ctttttgtga aaaatagaat
      181 aaatttgacg gcgcctcttt ggtgtgctaa cgttgtcaga caacagacat aggataaaga
      241 acacgtacgt caaaccggat ttttcaagga gagtgctcct cgtggaacca atcataccgt
      301 tccagcggaa gaaccgcagc gacatgatcc atgaaaagct tcacgaactg atacgtgagg
      361 ggcactggaa gcccggcgac aaatttccga ccgagatgga gctggcgaag ctcttcaacg
      421 ccggacgctc caccgttcgc gaagcgctca acctgctgaa ggcttcgaac cttctgaagg
      481 tcatcccggg acacggcact gtggtcgcca gtccttccga actcgaacac aacgtcctct
      541 cgcggcatat ccccgacagc cagaccgcac gggagctgtt caacatcgtc gaattccgca
      601 tctgccttga accgtccacc acctacctcg ccgcggaacg catcgttccc gcgcagtcgg
      661 acaggcttct cgaactcgca ggagcagtca acgacgacac attgacccct tcgggcttcg
      721 ccgagacgga catcgccttt cacgcattcc tcgcggagat cgcgggcaac cccctctgca
      781 tcgaaaccat gcgctccctt cacaacttct tttacaagca gcagatcgtc acgtcgctca
      841 tctccacacg aaaaaaaccg gtcgcccaga gccatatcgc catcgcgaag gccgtggcag
      901 accataacgc gaagcttgcg gagaaggaga tgcgcgcgca cttgtacaac acacggcagt
      961 acctggaaag catcgtacgg gagacgcacg tcccgaagaa tttcctaaaa acatccggag
     1021 gtgaatcatg aatcaggaga ctgtatcagc aatgaaagag aaggcgcgcg ccgtccgcct
     1081 cggcatcatc gacgcgctcg gcgtggacaa gcgcgggcat ctcggcggct ccatgtcgtg
     1141 cgccgacatc gtcgcggcgc tctacttcca caagatgcgc cacgacccga agaaccgctt
     1201 catggcggac cgcgaccgtt tcatcatgag caaggggcat tcggtcctcg cccagtacgc
     1261 ggcgcttgcg gaggcggggt atttcccgag ggagacgctg ctgaagacga agacgctcgg
     1321 cagcatcctc caggggcatc cggagcgcga aactcccggc gtggaggcga ataccggctc
     1381 gctcggacag gggctctcca taggtgtggg catggcgctc gccggaagac tcgacaaact
     1441 gaactaccgc acatgggtcg tcctcgggga cggagagctt gccgaggggc aggtctggga
     1501 ggcggtgacc gcggctgcgg cgttcaagct ggacaatctg accgccatcg tcgacatgaa
     1561 cggaattcag gcggtggggc gcacgtgcga ccgctacaac ctgggcaata tccgcggcaa
     1621 gttcgacgcc ttcggatgga agaccttcga gatcgacgga cacgacatgg cgcaggttct
     1681 ccaggcgctc gacgacgcgt ccgaggtcag aggagcgccc tccgcgatca tcgccggaac
     1741 agtcaaaggg aagggcgtct ccttcgcgga gaacacgccc gcctaccaca acaacctgct
     1801 gacgaaagaa cagcaggaag ccgcccgccg cgagatcatg gcgatgtgag ggggagaaaa
     1861 gacatgttga tgaatctacg cgccgcctac ggcgaagcgc tgctcgctct cgcgggggag
     1921 aataaaaacg tcgtcgctct cgacgcggat ctctgcggtt ccacgcagtc cgtcgtcgtc
     1981 gagaagaatt acccggagcg ctacttcgag atggggatcg gcgaacagaa tatgatctcc
     2041 gtcgctgcgg gacttgcgct cacagggaag ataccgttcg cccactcctt ctccgtcttc
     2101 gcaacggggc gccccttcga ccagatacgc cagagcgtct gcctgccgaa cctcaacgtg
     2161 aagatcgtcg gttcgagctg cggtctctcg gatttcggcg acggcgccac gcaccagtcg
     2221 ttcgaggacc ttgccacgat gcgcgtcctt ccgcacatga ccgttctctg cccggcggac
     2281 gggatcgagg tgaaatgggc cgtgcacgcc gcggcggcca ttgacgggcc ggtctatatc
     2341 cgtctgaacc gcaacgacct gccggtgctc ttcgacgaga agacgtcctt cgagatcggc
     2401 aagccgcttg tggtcaaaga ggggaacgat gtcgccctcg tctgcacggg gacgatgacc
     2461 tcgatagcgc tcgacgcggc agcgctcctc gaacagcagg gaatctccgc ccgcgtcgtc
     2521 catctcggga cgatcaagcc cctctgcggg aagtcgctcc gggaagctct cggaaacgtc
     2581 cgggggatcg tcagctgcga ggagcacaca atctacggcg gattggggtc ggcggtcgcg
     2641 gaggccctct gcgaggcgcc cctgccgatg aagatggtgg gcatccagga ccggtacggc
     2701 cagtcggcag tctgctacga ggagctgctt gagtgctacg gtctgaccaa agaggcagtc
     2761 gcgaaggcag cggaagcact gcttaaaaag taaaaagata ttcatacagg gaggcgattc
     2821 taatgagcac a
//
LOCUS       DAOPKU010000401         2828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_40401_length_2828_cov_4.931122, whole genome shotgun sequence.
ACCESSION   DAOPKU010000401 DAOPKU010000000
VERSION     DAOPKU010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40401_length_2828_cov_4.931122"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /locus_tag="PK849_11360"
     CDS             <1..2035
                     /locus_tag="PK849_11360"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66766.1"
                     /translation="LRLLRLPRIFRLLRFRHLKHSDAARRLSYRFRRLMEVSIFRQIL
                     TMLLVSFVFVWIFTTVLDGLGVQSEHGNNFWFSIITMISSDSIFEIHNQETLVKVLVL
                     IFSFIGIVLFNGVLIAIIIGKLMEHLDDLKQGRGDVRERGHIILLGWNECIPHIVDEL
                     ESYCRTERKRLIRVVVMREHPPESPDQVVESRPHVEVISRTGSFQNAQALERISAHKA
                     SAVVVLGGRAADRKLSERLNDPIVTRTLVALETLLDSKPNERHSPVIVLNYLDLSRSC
                     HVTEFLRPFGNRSTKVFFNPLFFTGKLIASMCINPYAEDIFNELLTPEGNEFHFVRLP
                     PGEKMLWRDMMNAFPKSIPVGYRDASGELRMVPRPDEEVPESAEVVVLSENACDASIF
                     TPPPPGRLPDARAPSPRCKNILPSGMLVIVGVNPQLPFIVEETCSLGMTVHIVDNQTK
                     EEFAAWYDEYSRTPLPESVVFRECRFRSEEEICAAIALASADRIILLADGHLLDAATP
                     DQIDAETISKLLMLGQLIEEQGREGIHLIVETLTLDSEVVVRNIRNCSNVIGPLTIGR
                     LLTTFTLQPEFEALFRTIIQYGEIDIACRPALDSVPGWKPGETTFADLLAGSRNSCIP
                     LGWVKLPEEGAALEATMRRAAPKVELNPPKSSIVPERSEIVFLHRQGEKTLRP"
     gene            2184..2828
                     /locus_tag="PK849_11365"
     CDS             2184..2828
                     /locus_tag="PK849_11365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPE66767.1"
                     /translation="MNATIVIRNEKEADYKRVEEITRKSFWNLYVPGCTEHYLVHVLR
                     THEDFLPELDFVIEVDGRIIGNIMYTKTLLVDESGEEKNILTFGPVCIVPEYQRKGYG
                     KRLMERSFERAVALGYDVIVIFGNPNNYVSRGFKSCKKFNVCLENGTYPAPMMVKELQ
                     PGALDGRKWVYHQSSVFEIDEHEAARFDESLEPWEKKYEPSQEEFYIHCHSVMQ"
BASE COUNT          624 a          858 c          796 g          550 t
ORIGIN      
        1 gcttcgattg ctccggctcc cgaggatctt ccggctgctc cgattccgtc acctcaagca
       61 ctcggacgcc gcgcgccggc tctcctaccg cttccggcgt ctgatggagg tctccatctt
      121 ccggcagatt ctgacgatgc ttctcgtatc gttcgtcttc gtctggatat ttacaaccgt
      181 tctggacgga ttgggcgtgc agtccgaaca cggaaacaac ttctggttct ccatcatcac
      241 gatgatcagc tccgacagca tcttcgagat tcacaatcag gagacgctgg tcaaggtgct
      301 cgtccttatt ttctcgttca tcgggatcgt cctcttcaac ggcgtcctca tcgccatcat
      361 catcggcaag ctcatggaac acctcgacga cctgaagcag gggcgcggcg acgtgcgcga
      421 acgggggcac atcatcctgc tcggctggaa cgaatgcatc ccgcacatcg tcgacgagct
      481 ggagtcctac tgcagaacgg aacggaagcg cctgatcaga gtggtggtga tgcgggaaca
      541 cccgccggaa tcacccgatc aggtcgtcga gtcgaggccg cacgtcgagg tgatctcgcg
      601 gacgggcagc ttccagaacg cgcaggcgct cgaacgcatc tccgcgcaca aggcgtcggc
      661 ggtagtcgtc ctcggaggac gggccgccga caggaagctc tcggagcggc tgaacgaccc
      721 catcgtgacc cggacgctcg tcgcgttgga aacgctgctc gacagcaagc cgaacgaacg
      781 gcattctccg gtgatcgtgc tgaactacct cgacctcagc agaagctgcc atgtgaccga
      841 gttcctgcgc ccgttcggca acaggagcac aaaagtcttc ttcaacccgc tcttcttcac
      901 cggcaagctg atcgcctcca tgtgcatcaa cccctacgcg gaggacatct tcaacgaact
      961 gctcacaccc gaggggaacg agtttcactt cgtccgcctg cccccgggag agaagatgct
     1021 ctggcgcgac atgatgaacg cctttccgaa gtcgattccc gtggggtatc gggatgcatc
     1081 gggcgaactg cggatggttc cccgccccga cgaagaggtt cccgagtccg ccgaggtcgt
     1141 cgtgctcagc gagaacgcct gcgacgcgtc gatcttcact ccgccgcctc ccggaaggct
     1201 ccccgatgcc cgcgctccgt cgccccgctg caaaaatatc cttccctcgg ggatgctcgt
     1261 gatcgtcggc gttaatccgc agcttccgtt catcgtcgag gagacatgct cgctcggtat
     1321 gacggtgcat atcgtcgata accagacgaa ggaagagttc gcggcgtggt acgacgaata
     1381 ctccaggacg ccgctcccgg agagcgtcgt cttccgcgag tgccgcttcc gctccgagga
     1441 ggagatctgc gccgccatcg ccctggcctc ggccgacagg atcattcttc tggcggacgg
     1501 ccatctgctc gacgccgcca cacccgacca gatcgacgcg gagaccatct cgaaactgct
     1561 gatgctcggc caactgatcg aagagcaggg gcgggaaggc attcacctga tcgtcgagac
     1621 gctcactctt gattccgagg tggtggtgcg caacatcagg aactgctcca acgtcatcgg
     1681 cccgttgacc atcggccgcc tactgaccac attcaccctc cagcccgagt tcgaggcgct
     1741 cttccgcacg atcatccagt acggagagat cgacatcgcc tgccgcccgg cgctcgactc
     1801 cgtccccggc tggaaaccgg gcgagaccac cttcgccgat ttgctcgcag gctcgcgcaa
     1861 cagctgcatc ccgctggggt gggtcaaact ccccgaggag ggggctgcct tggaagcaac
     1921 gatgcgccgc gccgctccca aggtggagct gaatccgccg aagagctcga ttgttccgga
     1981 gagatcggag atcgtttttt tacacaggca gggagagaaa acgctccgcc cgtagcatga
     2041 gcagaacggg agtataataa aaaattgtcg cttgccgagg ccttccagcg tcccgcaagc
     2101 gaacttctcc cggaaaggca aggatgttca tcgaatatcc ttgcgctact gtttcggagg
     2161 cataaaaata gtggaggctt tctatgaacg caacgatcgt catcagaaac gaaaaagagg
     2221 ctgattataa gagagtcgaa gagatcacca ggaaatcttt ctggaattta tacgtaccgg
     2281 ggtgtacgga gcactattta gtccatgtcc tacggaccca cgaagacttt ctgccggagc
     2341 tggattttgt aatcgaagtc gacggtcgaa tcatcgggaa tatcatgtat acgaaaacac
     2401 tgctcgtcga cgagtccgga gaagaaaaaa acatccttac cttcggcccg gtgtgcatcg
     2461 tgcccgaata ccagagaaaa gggtacggaa aaagacttat ggagcgctcc tttgaacgcg
     2521 cggttgcgct cgggtacgac gtgatcgtca ttttcggcaa ccccaacaat tacgtcagcc
     2581 gcggtttcaa aagctgcaaa aagttcaacg tctgccttga aaacgggaca taccccgcgc
     2641 cgatgatggt aaaggagctg cagccgggcg ccctggacgg aaggaagtgg gtataccatc
     2701 aaagctccgt gttcgagatc gacgaacacg aagccgcacg cttcgacgaa agcttggaac
     2761 catgggagaa aaagtacgaa cccagccagg aagaatttta cattcactgc cattcggtta
     2821 tgcagtga
//
LOCUS       DAOPKU010000402         2825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_40453_length_2825_cov_4.693502, whole genome shotgun sequence.
ACCESSION   DAOPKU010000402 DAOPKU010000000
VERSION     DAOPKU010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40453_length_2825_cov_4.693502"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PK849_11370"
     CDS             complement(<1..364)
                     /locus_tag="PK849_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPE66768.1"
                     /translation="MDYRIADIALAPSGEKKIGWARQYMPVLRHLEKKYKAEQPLAGT
                     VISACLHLEAKTACLLLLLKDLGAKVVAAGSNPLSTQDDVCAGLVKNGVAVFSRNGMA
                     TDEYFENARSALSVGPNIV"
     gene            complement(379..1398)
                     /gene="mtnA"
                     /locus_tag="PK849_11375"
     CDS             complement(379..1398)
                     /gene="mtnA"
                     /locus_tag="PK849_11375"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPE66769.1"
                     /translation="MLPQVISWEENRLSLLDQRRLPSSVEFLKCEAAEEVAVAIETLA
                     VRGAPAIGIAAAFGVALEARRGRAEALRAVQRLAVTRPTAVNLFWALRRVEERMKTVP
                     AEASNETLFSAVLEEATAIQREDLEGNRALGRAGAALLPDDCTVMTHCNAGALATGGH
                     GTALGVIRSAREAGKTVRVYACETRPLLQGARLTVWELMQDGFDVTLLCDNMAASLMK
                     SRRIDAVIVGADRIAANGDTANKIGTYSHALLAKHFGIPFYVAAPLSTFDASLPSGDR
                     IPIEERAADEVRRLPGGGMLPADYSIWNPAFDVTDASLISAIITEKGAIVPPFTEGIA
                     SFLSE"
     gene            complement(1419..2657)
                     /gene="xseA"
                     /locus_tag="PK849_11380"
     CDS             complement(1419..2657)
                     /gene="xseA"
                     /locus_tag="PK849_11380"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPE66770.1"
                     /translation="MMKRMPTTLTVDEISLRIASLFLKDPVMQAVGVRGELSELKKHS
                     SGHVYFTLLGKESRISCALFRSYASYVPEWPRNGDEVVAEGAVSVYAPRGGYQLLVKR
                     LVPLGKGAADRARLELMARLEAEGLFSPGLKRPFPSYPARVAVVTSPTGAALRDVIAV
                     ASKRMPSCELVIIPAAVQGYEAPEAIAEALGRIGAVAGAECAMLVRGGGSRDDLSAFD
                     DERVVRAVRNSPVPLVTGVGHEIDETLSDLAADLHAPTPSAAAERIFPDRRDILRLLD
                     HRLASARAALELDMSKSLRGIDALLSRGARTLEKDIMTRNASLQLSSSRLFSAAERAL
                     SESRERLAACAASLDGLSPLKAFDRGYIVCEKKGVPVLRAAELSSKDEVCLRFRDGDA
                     NAKIRSSSVRVVSQGDVADS"
     gene            complement(2623..>2825)
                     /locus_tag="PK849_11385"
     CDS             complement(2623..>2825)
                     /locus_tag="PK849_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPE66771.1"
                     /translation="FAKCTPVAVAISEAVELAKTFGTEESGKFVNGVLGRIVRNLPPD
                     AAAQDASAFDAGDETHADDADS"
BASE COUNT          587 a          953 c          789 g          496 t
ORIGIN      
        1 ccacaatatt cggccccact gaaagagcag aacgcgcgtt ctcgaaatac tcgtcggtcg
       61 ccatgccgtt tctgctgaag acggccacgc cgttcttcac cagccccgcg cacacatcat
      121 cctgggtaga gagcggatta ctccccgccg cgactacttt tgctccaaga tccttgagca
      181 acagcagcag gcaggccgtc ttcgcctcca ggtgcaggca cgcggagatc accgttccgg
      241 caagcggctg ctcggctttg tacttcttct caagatgacg gagcaccggc atatactgcc
      301 gggcccaccc gattttcttc tcgcccgaag gcgcgagcgc gatatccgca atacgatagt
      361 ccatgtgtgt tcttcctcct attcagaaag gaacgaggcg ataccctctg taaacggagg
      421 aactatcgcg cccttttccg taataatagc cgaaatcagc gaagcgtcgg taacgtcgaa
      481 cgccggattc cagatcgaat agtccgcagg cagcatcccg ccgccgggga gtctgcgcac
      541 ctcgtcggca gctcgctcct ctatcggaat gcgatcgccc gaaggcaacg acgcgtcgaa
      601 cgtgctcaac ggcgcggcaa cgtagaatgg aataccgaag tgcttcgcca gcagcgcatg
      661 cgaataggtc ccgatcttgt tcgccgtgtc gccgttggcc gcgattctat ccgcgcccac
      721 gatcaccgcg tcgattcgcc tgctcttcat aaggcttgcc gccatattat cacacaacag
      781 cgtcacgtcg aaaccgtcct gcatcaactc ccagacggtg agtcgcgccc cctggagcag
      841 cggacgggtc tcgcacgcat acacgcgcac agttttcccc gcctcccgag ccgaacggat
      901 tactccgagg gcggtgccgt gtcctccggt cgccagcgct cccgcgttgc agtgcgtcat
      961 gacggtgcag tcgtccggaa gcagcgcggc gcccgcgcgg ccaagggcgc ggttcccctc
     1021 cagatcctcc ctctggatag cagtcgcttc ctccagaacg gccgaaaaga gcgtttcgtt
     1081 cgaagcctcc gccggaacgg tcttcattcg ttcctcaacg cgccgaagcg cccagaagag
     1141 gttcaccgcg gtcggacgcg ttacggcaag acgctgaacg gcgcgaagcg cctccgctcg
     1201 ccctctgcgg gcttccagcg cgacgccgaa ggccgcggca ataccaatcg caggcgcgcc
     1261 tcgcacagcg agagtttcta tcgctacggc gacctcctcg gcggcttcac acttcaaaaa
     1321 ctctacgctc gacggcagcc tcctctggtc aagaagagac agcctgtttt cttcccatga
     1381 aataacttgg gggagcattt cacccctcct tctttgcatt aagaatccgc aacgtctcct
     1441 tgtgaaacaa cgcgcacgct ggaagaacgg atcttcgcgt tcgcatcccc gtcgcggaac
     1501 cggagacaca cttcgtcttt ggatgagagc tcggccgccc tgagcacggg gacgcccttc
     1561 ttctcgcaga ctatataccc ccggtcgaaa gctttcaacg gagagagccc gtcgagagaa
     1621 gcggcgcacg cggccagccg ctctctggac tccgagagcg cacgctccgc agccgaaaaa
     1681 agcctgctcg aagagagctg gagcgatgcg ttcctcgtca tgatgtcctt ctcaagagtt
     1741 ctcgccccgc gtgaaagcag cgcatcgatg ccgcgcagac tcttcgacat gtcaagttcg
     1801 agggccgccc gcgccgatgc aagccggtga tcgaggagac gaagaatatc ccgccggtcg
     1861 ggaaaaatac gctcggcagc cgcggaaggg gtcggcgcat gaagatcggc cgccaggtcg
     1921 gagagtgttt cgtcgatctc atgccccact ccagtcacca gcggaacagg agaattgcgg
     1981 acagcccgga cgactctttc gtcgtcgaag gccgacaggt cgtccctgct tccgcctccc
     2041 cggaccaaca tggcgcactc cgcccccgcg acagccccga tacgccccaa cgcttcggcg
     2101 atggcctcag gcgcttcata cccctgcacc gccgcgggga taatcaccag ctcgcaggaa
     2161 ggcatccgct tggaagccac cgcgatcacg tcgcgaagcg cggcccccgt cggcgacgtc
     2221 accacggcga cacgcgcagg gtaggaggga aacggacgtt tcaaacccgg ggagaaaagc
     2281 ccttccgcct caaggcgcgc catcagctcc aaccgtgcgc ggtccgcggc gccctttccc
     2341 agcggtacca gccgcttcac aaggagctgg taccctccgc ggggagcata gacggaaacc
     2401 gccccctcgg cgaccacctc gtcgccgtta cggggccact cagggacata cgaagcataa
     2461 cttctgaaaa gagcgcacga aatacggctt tccttcccca ggagggtgaa atagacatga
     2521 ccgctggaat gcttcttcag ttcgctcaac tcgccgcgaa cgccaacggc ctgcataacg
     2581 gggtccttca gaaaaagcga ggcgatacgg agggaaatct cgtcaactgt cagcgtcgtc
     2641 ggcatgcgtt tcatcaccag cgtcaaatgc ggacgcatcc tgcgcagccg catcaggagg
     2701 aagattccgg acgatacgcc ccagaacgcc gttgacgaac ttgccggatt cctcggtacc
     2761 gaaggtcttc gccagttcga cggcctccga aattgcgacc gcgacgggag tgcattttgc
     2821 gaaca
//
LOCUS       DAOPKU010000403         2817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_40634_length_2817_cov_4.253077, whole genome shotgun sequence.
ACCESSION   DAOPKU010000403 DAOPKU010000000
VERSION     DAOPKU010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40634_length_2817_cov_4.253077"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(383..595)
                     /locus_tag="PK849_11390"
     CDS             complement(383..595)
                     /locus_tag="PK849_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66772.1"
                     /translation="MNTEYTAVMKQDGDWWIGWIEEIPGINCQERTRDELTETLRITL
                     REAIELNRSDAVDAAGEGYTKVKIAV"
     gene            complement(733..1779)
                     /locus_tag="PK849_11395"
     CDS             complement(733..1779)
                     /locus_tag="PK849_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE66773.1"
                     /translation="MNHAVELLSNLVAIPSLSGREEHACAFLADALPAAGWERVEIDG
                     AGSVVASRGSGSRELILMGHIDTVPGGPAYALDGDILRGRGSVDAKGPLCSFAVAGGR
                     APIAPDWKLTLIAATGEEADSRGALHRIPKHSPAACIIGEPSGADGVTIGYRGSLRIA
                     LSAEDGGAHRSGDAGPITAVVRAAADILSFVESADSPELPVIQRPSGAVVSMHGEENG
                     RRLGRSDLDIRLPEGSDPQQWIERIVELAGRRGVHARTLAVTPAHVADKNNQAARALR
                     LAVRKNGLSPRLLAKGGTADFNLAAAWNCPIAAYGPGDSKLDHTAEEHISLSEYLTSL
                     DILTDAIPMIMRSA"
     gene            complement(1776..>2817)
                     /locus_tag="PK849_11400"
     CDS             complement(1776..>2817)
                     /locus_tag="PK849_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE66774.1"
                     /translation="LDFYCGSGAALFGHAHPVLKQALVEAAELPWTIGLGMGSAVRDS
                     FKARLNDILPDRSVFYCNSGTEAIEAALKLATSLRPGKKKVLALRRAFHGRTIGALSL
                     TFNPQYRKEWAHLLPPVQHVKPEELPGAVDADTAVVFVEPVQGEGGVHPLAPSLGEAI
                     TEACRTTGALLVSDEIQSGWGRCGALSASPLAGLEPDILCFAKGVAGGMPIGLMLWKK
                     ELGDFSASGHGSTYGGNPLSLAVANASLKLLDDEGYPEKARAGGEFFRTLLAEIDSPL
                     ISEIRGMGLLNGVELTVKAVPVVKRMQEMGVLALPAGPSVVRFLPPFPAGDADFLKAA
                     SVFAEALKEEGR"
BASE COUNT          470 a          906 c          932 g          509 t
ORIGIN      
        1 acgctggaaa gagcgtacct ggcaaccgga gtgaacgcgt cttcgaatcg aaggacggaa
       61 attgagcgcg gtagaaatgg ctgaaagagg cagtgcagtc aggcgcaacc acagcggtac
      121 agtcatgcgc aacaaaattt tcgtcggagc ggactttcgc cggacggcgt tcgggccgtc
      181 gagtatcttg gcctgtccta ttgaacgggc ggcactccga gatctttgca gatcttcttc
      241 gccaggagat tcgatatctc cgagtgtcgc ggaacagctg agcgcgtatt caaggaggcg
      301 ttgtgccacc atgagtggtt ggcgccttcc cgtaacagga cgcacccctg tccgcgcagg
      361 tagcgaagca acacttcccg cttcataccg cgatttttac cttcgtgtag ccttcgcccg
      421 ccgcatcgac cgcatcgctc cggttgagtt caatcgcttc gcgcagggtg atgcgcagcg
      481 tttccgtcaa ctcgtcgcga gttctctcct ggcagttgat tcccgggatt tcctctatcc
      541 agcctatcca ccagtcgccg tcctgcttca ttacggcggt gtattccgta ttcatgtaca
      601 tcgctccttt actcttcgcg atggttgccg ggtgctactc cctgcatcga gtcggccacg
      661 cgctcttgaa tagagaaagc atagtattga tgcggaataa ttgcaagcgt atgcgcatca
      721 tgaggagcgc gttcaggcgc tcctcatgat catcggaatg gcgtccgtca gaatgtcgag
      781 ggaggtcagg tactcggaga gcgaaatatg ctcttcggcg gtgtggtcga gcttgctgtc
      841 gccggggccg taggcggcga tggggcagtt ccacgcggcg gcgaggttga agtcggcggt
      901 gccgcccttt gcaaggagcc tgggactcag gccgtttttc cggacggcga ggcgcagagc
      961 gcgggccgcc tgattgttct tgtcggcgac gtgcgcggga gtgacggcga gggtacgtgc
     1021 atgtacgccc cggcgtccgg cgagttcgac gatgcgttcg atccactgct gcggatcgct
     1081 tccctccggg aggcggatgt cgaggtcgct cctgccaagc cggcggccgt tctcctcgcc
     1141 gtgcatggag acgacggcgc ccgaaggccg ttgaatgacc ggcaactccg gggagtcggc
     1201 ggactccacg aacgagagta tatccgccgc cgcccggacg accgcggtta tggggcccgc
     1261 gtcgccgctc cggtgcgcgc ccccgtcctc tgccgagagc gcgatgcgga ggctccccct
     1321 gtagccgatg gtgacgccgt ccgccccgga gggctcgccg atgatgcagg cggcggggga
     1381 gtgtttgggg atgcggtgga gcgcgcctct ggagtcggcc tcctcgccgg ttgcggcgat
     1441 gagcgtgagc ttccagtcgg gagcgatcgg cgcgcgtccg cctgcgacgg cgaaggagca
     1501 gagcggcccc ttcgcgtcga cgctcccgcg gccgcggaga atatccccgt cgagcgcgta
     1561 ggccgggccg ccgggcacgg tgtcgatatg ccccatgagg atgagttcgc ggcttcccga
     1621 tcccctggag gcgacgacgc tccccgcgcc gtcgatctcc acgcgctccc atccggccgc
     1681 cggaagcgcg tcggcaagaa aggcgcacgc gtgctcttcc cgcccgctca gactcgggat
     1741 ggcgacaagg ttcgacagca gttcgacagc atggttcacc gcccttcctc cttcagcgct
     1801 tcggcgaaga cggacgcggc cttcaggaag tccgcgtcgc ccgcggggaa ggggggaaga
     1861 aaccggacga cggaaggtcc ggcagggagg gcgagcacgc ccatctcctg catccgcttc
     1921 accacgggaa cggccttgac ggtgagctcc acgccgttca gcagccccat gccccggatt
     1981 tccgaaatca acggcgagtc gatctccgcc agcaacgttc tgaagaattc tccgccggca
     2041 cgcgcttttt cagggtagcc ttcgtcgtca agcagcttca gcgaagcgtt cgccacggcg
     2101 agggagagcg ggttgccgcc gtacgtggag ccgtgtccgg aggcggagaa atctcctagt
     2161 tccttcttcc agagcatgag cccgatcggc atcccccccg cgacgccctt cgcgaagcag
     2221 agaatatccg gttccagccc ggcgagcgga ctcgcggaga gagcgccgca gcgcccccag
     2281 ccgctctgta tctcgtcgct gacaagaagc gcgcccgttg tacggcaggc ctccgtgatc
     2341 gcctccccga gcgacggggc aagcggatgg acgccccctt cgccctgtac cggctcgacg
     2401 aagacgacgg ccgtatcggc gtccaccgcg ccggggagct cttccggctt cacgtgctgg
     2461 acgggcggca gaagatgcgc ccactccttc cggtactgcg ggttgaacgt gagcgagagc
     2521 gccccgatcg tccgcccgtg gaaggcgcgc cgcagcgcaa gcaccttctt tttccccgga
     2581 cggagcgacg tcgcgagttt cagcgcggcc tctatggcct ccgtgccgct gttgcagtag
     2641 aagacggaac ggtccgggag gatgtcgttc aggcgcgcct tgaagctgtc gcgcactgcg
     2701 gaccccatgc caaggccgat ggtccacggc agttccgccg cctcgacgag cgcctgcttc
     2761 agtacaggat gcgcgtggcc gaagagcgcc gccccgctgc cgcagtagaa gtcgagg
//
LOCUS       DAOPKU010000404         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_40744_length_2811_cov_4.357765, whole genome shotgun sequence.
ACCESSION   DAOPKU010000404 DAOPKU010000000
VERSION     DAOPKU010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40744_length_2811_cov_4.357765"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PK849_11405"
     CDS             <1..841
                     /locus_tag="PK849_11405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE66775.1"
                     /translation="FRGRFHREASSRENPPLLAILDWMLPGESGPEICRKLASRERLR
                     EGNAAREEMMKPYQYVIILTVKGEKENVVAGLEAGADDYITKPFDAHELKMRLMVGRR
                     ILDLQEKLRNAALFDALTGLLNRRAVIERLEQELARASREATPLSVALLDIDHFKDVN
                     DTYGHASGDAVLVESAQRIRSSVRPYDIVGRYGGEEFLMVFPGAEGSALDGICERIRN
                     AFQGAPFYSAENDGDRSTMDITVSIGISEYKKDNDNVDFILAEADRAMYRAKRSGRNR
                     VSR"
     gene            1267..>2811
                     /locus_tag="PK849_11410"
     CDS             1267..>2811
                     /locus_tag="PK849_11410"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPE66776.1"
                     /translation="MALALLPKAPADLGYYVAYALFRLADEKDHDGRKKYLQRMYALA
                     ENNAQRTTALTNLLKLPGNKEAYALNLTNISPRNSAALKVLESLPKPWNPDVNMAVGY
                     AAYLRGQYDKAILLFRAIPMDSRHGRKSRYYRAFSLYNQKKYTAALEAWGYLAKTGTS
                     YAESSIRRISILAGRAERESALSVLREVAASRDGEIQARAYYSLSTHLSGKEKREAED
                     KVIALAPNSLFTTQILWRRGWGKWASGDVRGAVADWERSLVPEMKTSWRPRVLYWVSK
                     GYGRLNQPSKRDTFVKTLLKDHPLSIYAFLSGGEKLDIQAGDPPSLASRQPSELEKWG
                     FVSHARRVLLAKGDAPSIYRAALLAEWMGDQQAAYSAAGRVSEQIRKGPVFYRRALSF
                     LFPRPFFSEVTKASDRFKVEDNIVWAIMRQESAFNPNATSWVGAGGLMQLMPGTARSE
                     AKALEMKKYSVYNPEQNIILGSAHIARLLRSFGDVEVALAAYNAGGGNARKWLDGRKD
                     VPLDEWI"
BASE COUNT          681 a          694 c          810 g          626 t
ORIGIN      
        1 atttcggggc cgctttcacc gggaagcatc cagtcgagaa aacccgccgc tgctggcgat
       61 actcgactgg atgcttcccg gtgaaagcgg ccccgaaatt tgccgaaaac tcgcctccag
      121 agagcgctta agggagggga acgccgcccg ggaagagatg atgaaaccat atcaatacgt
      181 tatcatcctc accgtgaaag gagagaagga aaatgtggtt gccggtttgg aagcaggagc
      241 cgacgactac ataacaaaac cctttgacgc acatgaactg aaaatgcggc tcatggtagg
      301 gaggcgcatt cttgaccttc aagaaaagct tcgcaacgcc gctcttttcg atgcattaac
      361 aggacttctc aacagaagag ccgtcattga gcgtcttgaa caagaactgg cgcgggcatc
      421 cagggaagca actcctttga gtgttgcgct tttagacatt gatcatttta aggatgtcaa
      481 cgatacttac gggcatgcct cgggagatgc ggtgctggtg gaaagtgcgc agcgaatacg
      541 ttcttcagtc cgtccgtacg atattgttgg cagatacggc ggggaagaat ttctcatggt
      601 ttttcccggc gccgagggaa gcgcgctgga tggtatctgc gaaagaatac ggaatgcttt
      661 ccagggagct ccattttatt cggccgagaa tgacggtgat cggtcaacga tggatataac
      721 agtgagtatt ggtatttctg aatataaaaa ggataatgat aatgttgact ttattctggc
      781 tgaagcagac agagcaatgt acagagcgaa gagaagcggg agaaataggg taagccgctg
      841 actactgtcg gtaattctac cggagcggca ggaagctctc tcacgtagaa tggccgggat
      901 attcgccgaa aggtgttgag aggagtgttt tcggtgtccc gacgattgtt tcgtctttct
      961 ttgttttttg tgttctctgc gttgcttttt gtcttccctt tgagtgcata cagccagtca
     1021 atggaagatc ttttccaacg ccgggactgg aagggaattg attcccttct tgtttcggga
     1081 aatgctctca cggccgtgga ttactcactt gccgccaatg ctctgtggtt ccagaatcgg
     1141 tggagtgaat cacttgttac tctcaaaaag agcgcatctt catggccatc cggggtaaaa
     1201 ccatacgggg attttatgat tgcacttgcc ctggagaggg caggacaaaa aaatgaagcc
     1261 cgtaaaatgg ctcttgcact cttacctaaa gctcctgcag atctcgggta ctatgtggct
     1321 tacgctcttt ttcgcctggc cgatgaaaag gatcacgacg gaaggaagaa atacctccag
     1381 cggatgtacg ctcttgcgga aaacaacgca cagcgcacga ctgctctgac aaatcttcta
     1441 aagcttccgg gcaacaaaga ggcgtacgct ctgaacctga cgaacatctc gcctagaaac
     1501 agcgccgctt taaaagtttt ggagtctctt cccaaaccat ggaatcccga cgtcaatatg
     1561 gccgtgggat acgcggcgta tctccggggg cagtacgata aggcgattct cctcttccgg
     1621 gcaattccaa tggattcaag acacggcaga aagtcgcgct actaccgtgc gttttcattg
     1681 tataaccaga aaaagtacac cgccgccctg gaggcgtggg gatacctcgc gaaaacaggg
     1741 acgagttatg ccgaatcttc gatccggcgg atttctattc ttgcagggcg ggcggagaga
     1801 gaaagcgctc tcagcgtgct ccgggaagtt gctgcgtcca gagatggcga gatccaggcc
     1861 cgggcctatt attccctctc tacccacctt tccgggaagg agaaacggga ggcggaagac
     1921 aaagttatcg cgctcgctcc caactcgctg ttcactacgc agatactgtg gagaagagga
     1981 tggggcaagt gggcttcagg agatgtccgc ggggcggttg ccgattggga gagaagcctt
     2041 gtccccgaga tgaaaacgag ctggcggccg agggtgctct actgggtctc caaggggtac
     2101 gggcgtttga atcagccctc gaagagagat acgttcgtga agacgctcct caaagatcac
     2161 cctctctcca tatacgcctt tctgagcggc ggggagaagc tcgatataca ggccggagat
     2221 cctccctcgc ttgcatcccg ccagccttca gagcttgaaa agtgggggtt tgtctcccat
     2281 gcgagacgcg ttctcctggc gaaaggagat gctccttcga tataccgtgc ggcgctcctt
     2341 gcggagtgga tgggagacca gcaagccgct tactccgcag ccggcagagt atcggaacag
     2401 atacggaagg ggccggtttt ttacagaagg gccctctcct tcctctttcc ccgaccgttc
     2461 tttagcgaag tgaccaaggc atctgaccgt ttcaaagtgg aggataacat cgtctgggcg
     2521 atcatgcgtc aggagagcgc cttcaacccg aacgcgacaa gctgggtcgg cgcaggcggc
     2581 ctgatgcagc tcatgccagg cacggcgcga agcgaggcga aagcgctgga gatgaaaaag
     2641 tatagcgtct acaacccgga gcagaacatc atcctgggca gtgcgcacat tgcgcgtctt
     2701 ctgcgctctt tcggcgacgt tgaagttgct ttagcggcat acaacgcagg aggaggaaac
     2761 gcccggaaat ggcttgacgg tcggaaggac gtgccgctcg acgagtggat t
//
LOCUS       DAOPKU010000405         2775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_41455_length_2775_cov_7.313603, whole genome shotgun sequence.
ACCESSION   DAOPKU010000405 DAOPKU010000000
VERSION     DAOPKU010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41455_length_2775_cov_7.313603"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..425)
                     /locus_tag="PK849_11415"
     CDS             complement(168..425)
                     /locus_tag="PK849_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66777.1"
                     /translation="MKAKQFDEAFDKGEDITLHLDLDGARRSSRKQKRINVSFPEWML
                     VSLDLEASRLGIPRQAIIKLWLSERLKSASDENGTDQHPVA"
     gene            508..1038
                     /locus_tag="PK849_11420"
     CDS             508..1038
                     /locus_tag="PK849_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66778.1"
                     /translation="MFSGNTTDNKKARFVLDLCRNFEQIGIAPKLSGILEIDTMFFEV
                     AFTFCSSESGDANTLSTFVRPLLYSEWNFQVLKEQNLAKHLKRRFHRERIERGISCKL
                     LVDNCRRRRAATRGNCRGILGSSNRGGTRGAPLFLDYEQRRNTEEAQAADAQDEAELQ
                     ALGNRIRDCKEDMKST"
     gene            1302..2489
                     /locus_tag="PK849_11425"
     CDS             1302..2489
                     /locus_tag="PK849_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HPE66779.1"
                     /translation="MLYLGIDIGKNNHEAGIVDGEGKQRGKSLRFANDQQGFFSLTSF
                     LEQKVGPGEELRVGMEATGHYWIPLYSFLHGKGFILHVINPIQSDSLRNFTIRGAKTD
                     SIDCFLIAHTIRFGDFSETRLADEDVLTLRNLARFRESLKDSCANYKRQVVTVLDQVF
                     PEYDALFSNMFGESSKAFLKTYGTPEQTMELDTKSLAALLRKASRGRHGTKKAEEIKA
                     LAGRSIGVPFCKDAFAFQLKILIEQIEFTEKQIGEIDKKLAQLLKKLNPVILSIPGVG
                     PATGAVILGEIGDINRFDKPKKLVAFAGIDPTVMQSGNFSGSHNRFSKRGSPYLRRAI
                     WLAAVVAARFDPVFKAFYEKKRGEGKAYGTAIGAVARKLAYTIFAILKTNKPYEVRLN
                     PEA"
BASE COUNT          640 a          768 c          716 g          651 t
ORIGIN      
        1 aaatcatata tgtgttctgg gaaaatacct ccggggagag attgactgtc caaggattca
       61 ccagagttca ttcatgacat ccttcagcgg aatatccttc ccctcggcga actccgcccg
      121 agaaatcgcc acagactcgc tctgctgcgt gatcgtcgtt ctttcgccta tgccacgggg
      181 tgctgatcgg ttccgttctc gtccgaagcg gatttcagcc gttccgacag ccagagtttg
      241 atgatcgcct gccgggggat gcccaagcgg ctcgcttcca agtccagcga caccagcatc
      301 cattctggaa aagaaacatt tatccgtttc tgcttcctag atgatcgtct tgctccgtcg
      361 agatcaagat gaagggtaat atcctcgccc ttgtcgaagg cttcgtcaaa ctgttttgct
      421 ttcatagaac cgtacctcct ccttgcggga tcttcgcacc gaaattatcc ggactcgttc
      481 ctgccggtac gtgatcacgg cggaccagtg ttttccggca atacgaccga taacaagaaa
      541 gcgaggttcg tcctcgacct ttgccggaat ttcgagcaga tcgggatcgc tccaaagctg
      601 tctggcatcc tggaaatcga taccatgttt ttcgaggttg cgttcacttt ttgttcctcg
      661 gagagcggcg acgccaatac gttgtccacg ttcgtccgcc cattattata ctctgaatgg
      721 aattttcagg tactcaagga gcagaatctt gccaagcacc tgaagcgccg ttttcaccga
      781 gaacgaattg agcgaggaat cagttgtaaa ctattggttg ataactgccg ccgacggcga
      841 gctgcaacgc gcggcaactg ccgaggaatc ctcggtagtt caaaccgagg ggggacgcga
      901 ggcgccccgc tgtttctgga ctacgaacaa cgccgaaaca ccgaagaagc ccaagctgcc
      961 gacgcgcagg acgaggccga gctgcaagcg ctgggaaaca ggataaggga ttgcaaggag
     1021 gatatgaaga gcacataaaa ccctcctaga ataaagtagt tgcaactggg atcagcaaat
     1081 agcccgtaga gttgcgataa tgtcagcatg caggccgagt gtcgccggtt gtttccttgg
     1141 ggtctgaacc tcgttgagga gtctggcgcc tcgcctcttg cggctttgca cggatgagct
     1201 ggatgttgcg gccgctcgcg gtcttcttcg tctgtccaag gaggttgtgc gccgtcggag
     1261 tgcgaggatc ccctgcaaat tcactatacg gagtgtgcgt tatgctgtat cttggtatcg
     1321 acatcgggaa aaacaaccac gaagccggaa tcgtcgacgg ggaagggaag caacgaggga
     1381 agtccttgcg gttcgccaac gatcagcagg gattcttctc tttgacgtcc ttccttgagc
     1441 aaaaggtcgg accaggcgag gagctgcgcg tcggtatgga agctaccggc cattactgga
     1501 ttcccctgta ttccttcctc cacgggaagg gctttatact ccatgtcatc aaccctattc
     1561 agtccgacag tctcaggaac tttaccatac gaggcgcgaa aacagactct atcgactgtt
     1621 tcctgatagc acacaccatt cgcttcgggg acttctccga gacccggttg gccgacgagg
     1681 atgttctgac gcttcgtaat ctggctcgtt tccgcgaatc gctcaaggac agctgcgcga
     1741 actacaaacg tcaggtcgtc actgtactcg accaagtctt ccccgagtac gacgcgctct
     1801 tctccaacat gttcggcgaa agctcgaagg cttttctcaa aacatacgga acacccgagc
     1861 aaacgatgga actcgacacg aaatctctcg ccgcattgtt gcgaaaggcc agcagagggc
     1921 gtcacggcac gaaaaaagcg gaggagatca aggcgcttgc gggacgctcc atcggagtcc
     1981 cgttctgcaa ggacgccttc gctttccagc tgaagatcct tatcgagcag atagagttca
     2041 cggaaaagca gatcggcgag atcgacaaga agctcgccca attactgaag aaactgaatc
     2101 cggtcattct ctccattccg ggcgtcggcc ccgccacggg cgccgtcatt ctgggcgaga
     2161 tcggagacat caaccgcttt gacaaaccca agaagcttgt cgccttcgcg ggaatcgacc
     2221 ctaccgtgat gcagtccggc aacttctcgg gaagtcacaa tcgcttctcc aaacgaggct
     2281 ccccgtacct gaggagagcc atctggttgg ccgccgtcgt cgccgcccgc ttcgacccgg
     2341 tcttcaaggc attctatgaa aagaaaagag gcgaagggaa agcttatggt acagccatcg
     2401 gcgctgtcgc cagaaagctt gcgtacacca ttttcgcgat cctgaagacg aacaagcctt
     2461 atgaagtccg actgaatccg gaagcataat tcccaatctg caatatgctt cgtgtttctc
     2521 cgatctgttt tccggtcgga tcaatttcgc atactctttt tcaaggctat attatgctgt
     2581 caagttgcag tcatgcatac tgccttaaaa tatcctgccg acaatctctt tactttttct
     2641 caaccaaccc tattgacttt tgatagctgg tctcctctat gatgtgagta tgccattaca
     2701 gcctttttta ggatctgccg gatcagatac gcgccactcc tgtttttcgc gcggcttcgg
     2761 cgacggcttt cgcga
//
LOCUS       DAOPKU010000406         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_41478_length_2774_cov_4.634057, whole genome shotgun sequence.
ACCESSION   DAOPKU010000406 DAOPKU010000000
VERSION     DAOPKU010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41478_length_2774_cov_4.634057"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PK849_11430"
     CDS             <1..868
                     /locus_tag="PK849_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66780.1"
                     /translation="RVIYSDDGGKDGNERLFHEYGSINANNWASFILVYPVEERERFA
                     PIVACLEETLMIAPEDAGGKELRTDAFALVNGRVQKDGTPLDCEVYDIPSGLDNGIAN
                     WAVIGTGTSDAVTEKETGVWFFGSEGEFMTFIPLDNENEYQDLLWSPAGDRLILVRGS
                     GARPDMFFDVYAEGMEKQAEFSGLREEIAWLDDGMRIVFTRIDGIREGGAFPGLSYGL
                     QLSVILYDSAVEETIPLKEASVTKNFSFVSVSEDGEKITILETSVRSPKDWAGEEKIN
                     EREISVPVPAAG"
     gene            1049..>2774
                     /locus_tag="PK849_11435"
     CDS             1049..>2774
                     /locus_tag="PK849_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66781.1"
                     /translation="MRETENRLPRSETTFVFPQRLSARLDGISAFPVTIVEAPSGFGK
                     TTAVREFLKSLQHNSRQSWYTCFGEHPSKVWEGVCRLFWNVDIDVARRLKELFPPTVE
                     TLPDIAELMRECRCDAATFLVIDNYQLFESPIPYHIAHAFSAHSNPNLHMLFITQPLP
                     TFEIAVQDANIHRLGTRDFFFDRENTARLCRTAGARMTEKEIDRIQSVSEGWVSAILL
                     QASNYMETGAFADARDMDALVETAIWNRMTGEEHEFLLGLSLLDAFSPRQAAIMAGGP
                     TLPEHLLKMLKNGFFISYIADKGVYSLHSILNDYLKQRFENQPREFAEIMIRRAGAAC
                     AAASDYFPAAQFFSRVRDYGAILSMPLTTSYLNEQKEKDIVGFLERFVGECPEQTLQE
                     YPFALLTFAFQFMKSGKRALFSQMVRLLRAIADAPSGLPESTVSRIRGETALLLSFTE
                     FNDIAKMSAYHREALLHLNMSDDGDPPKTVVFGSTPWTFGVASVLCLYWSKSGELDAE
                     LALMGECMPWYVKLSAGHGAGADSVMRAEACLYRGEETEAEAASHKALYQARSARQRV
                     SHRRPDSQV"
BASE COUNT          638 a          784 c          791 g          561 t
ORIGIN      
        1 cagagtgatc tacagcgacg acggcgggaa ggacgggaac gaacgcctct tccacgaata
       61 cggaagtatc aatgcgaaca actgggcttc gttcatcctc gtctaccccg ttgaagagcg
      121 ggaacgtttc gcccccatag tcgcgtgcct ggaggaaacg ttgatgatcg caccggagga
      181 tgccggcggc aaggaactcc ggacagacgc ttttgctctc gtaaacggcc gcgtgcagaa
      241 agacggaacg cctcttgact gcgaagtgta cgatattcca tccggactgg acaacgggat
      301 cgcgaactgg gccgtaatcg ggacgggaac atccgacgca gtaacggaaa aagaaacggg
      361 cgtctggttt ttcggatccg aaggagaatt tatgaccttc atccctctgg acaacgaaaa
      421 cgaataccag gatcttcttt ggagccctgc gggcgatcgc ctgatcctcg tccggggaag
      481 cggagcaaga cccgacatgt tcttcgacgt gtacgcggag ggcatggaga aacaagccga
      541 gttcagcggc ctgcgcgaag aaatcgcgtg gctggacgac ggaatgcgca tcgtctttac
      601 caggatcgac ggtattcgcg aggggggtgc gtttcccggc ctttcctacg gtctgcaact
      661 gtccgttatc ctgtatgatt cggccgtgga ggaaacgata cctttgaaag aggcttccgt
      721 cacaaaaaac ttctcgtttg tatctgtttc ggaagacggg gaaaagatca cgatcctgga
      781 aacttcggtc cgctctccca aagactgggc cggcgaagaa aaaattaacg aacgggaaat
      841 atccgttccc gtgcctgccg cgggataaag agtcccgcga tttctccgcg ttgacgccgt
      901 tgcgcacctg caacaacgag cgacaatcag aaggctgtgc acattcccgg cgtttgctgt
      961 aaactttttg gagacgctcc gacattatag ccggagcgtc tccaaaaagt tttgcatttt
     1021 actatcggga tgaacggaga gagacgccat gcgagagaca gaaaaccgat taccccgtag
     1081 cgagacgacg ttcgtcttcc ctcaacgcct gtcggcacgc ctggacggaa tatccgcgtt
     1141 cccggtcacc atagtggaag cgccgtccgg cttcggcaag acgacagccg tcagagaatt
     1201 tttaaagagt cttcagcaca acagcagaca gagctggtac acatgcttcg gcgaacaccc
     1261 ctcgaaggtg tgggaaggtg tgtgcagact gttctggaat gtggatatcg acgttgcgcg
     1321 aagactgaag gagttgttcc ccccgacggt cgaaacgctt cccgatatcg ccgagctgat
     1381 gcgcgagtgc cgctgcgacg ccgcgacgtt tcttgtgatc gacaactatc aactgtttga
     1441 aagtccgatc ccgtaccata ttgcgcacgc cttttccgcg cattcaaacc cgaatctcca
     1501 catgctcttc ataacccagc ctcttccgac ttttgaaatc gcggttcagg acgcaaacat
     1561 acacagactg ggaacgagag atttcttctt cgaccgcgag aatacggcgc gactctgccg
     1621 tacggccgga gcacgcatga cggagaagga aatcgaccgg attcagagcg tctccgaagg
     1681 atgggtctcc gccatcctgc tccaggcctc gaactacatg gaaacggggg cgttcgccga
     1741 cgcgcgcgac atggacgcgc tcgtcgaaac ggcgatatgg aacagaatga ccggggaaga
     1801 acatgaattc ctccttgggc tctctctcct cgacgctttc tcccccagac aggcggctat
     1861 catggcaggg ggcccgacct tgccggaaca cctgctcaag atgctgaaaa acggtttttt
     1921 tatctcgtac atcgcggaca aaggcgtcta ttctctccac agcatcctga acgactatct
     1981 gaaacagcgc ttcgagaacc aaccccgcga atttgcggag atcatgattc gcagggcggg
     2041 cgcagcctgc gccgccgctt cggactactt tccggccgcg cagtttttct ccagggttcg
     2101 ggactacggc gccattctct caatgccgct cacgacaagt tatttgaacg aacagaagga
     2161 aaaagacatc gtcggctttc ttgaacgatt cgtaggagaa tgcccggaac agactcttca
     2221 agaatatccg ttcgccctcc tcaccttcgc gtttcagttc atgaagagcg gcaaaagggc
     2281 gctcttttcg cagatggtcc gcctgttgcg cgcgattgcg gacgctccct ccgggctccc
     2341 ggaatcgacg gtgtcgcgta tcagggggga aacggcgctg ctgctctcct ttacagagtt
     2401 caacgatatc gcgaaaatga gcgcatacca tagagaggcc cttctccatt tgaacatgtc
     2461 cgacgacggc gatcccccga aaaccgtcgt gttcggctcg acgccgtgga cattcggcgt
     2521 tgcgtcggtg ctctgcctct actggagcaa gagcggggaa cttgatgcgg aactggcgct
     2581 gatgggggaa tgcatgccct ggtacgtaaa gctgtccgct ggtcacggcg cgggcgcgga
     2641 cagcgtgatg cgggccgaag cctgcctgta ccggggcgag gagaccgaag cagaggcggc
     2701 aagccataaa gcgctctacc aggcccggag cgctcgtcaa agggtctccc atcgtcggcc
     2761 cgattcgcaa gttc
//
LOCUS       DAOPKU010000407         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_41481_length_2774_cov_3.970210, whole genome shotgun sequence.
ACCESSION   DAOPKU010000407 DAOPKU010000000
VERSION     DAOPKU010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41481_length_2774_cov_3.970210"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PK849_11440"
     CDS             <1..1360
                     /locus_tag="PK849_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HPE66782.1"
                     /translation="LRLYAKERGIAHLDLPAEKFVSRIMGFLTPRNSEVTREFRRLME
                     QEGAAAAVAWFYRFSADTMYVRMDLVSLDRKWTAMTEFGEMQITINRSKPEKDPRDIR
                     AAGAQTGEDRYPKCLLCAENAGYQGRPGHPERSNHRIVPLELSGEQWYMQFSPYVYYH
                     HHCIVFSKEHRPMRIDRMTFVRICDFLTLFPGMFIGSNADLPIVGGSILSHDHFQGGT
                     WTFPMTNAPVLKSLRVAGASLEHLRWPLTTLRLRSPDRTVLETLGEQLLLSWREYEDR
                     ERGILARSGNGEREEQHNTVTVIGRRRGAEYEIDVVLRNNRCDTQYPDGIFHAHRERH
                     HIKKENIGLIEVMGLAILPPRLESSLNDVTAILSGEKTLSDLPQYFPHTEWTVELATR
                     FGTTLPADEAERIVRNEVGTVFSKVLRDCGVFKATEDGEKGVERFLSSWAAQWGRSFA
                     SD"
     gene            complement(1448..>2774)
                     /locus_tag="PK849_11445"
     CDS             complement(1448..>2774)
                     /locus_tag="PK849_11445"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE66783.1"
                     /translation="EAVTILMLGGVFVLVMTGFPIAFVIGSVAFFTGLAVFGPTVTFH
                     ILYSRFYDLSLNYPYLAVPLFTFMGVILQHSGVTKDLYQSLYEAMGRLRGGLAVVTII
                     FGTILAACLGVIAASVTILTLIALAPMITRGYDKSLASGSIVAAGTLGILIPPSIMLV
                     VYAPQAGLSVGQMFMGAVFPGLLLSACYILYVVIRCRLNPALGPAIPEDQITPFDAKK
                     MIRLLKSLVPPILLIIAVLGTIFAGIAPPTEAAAVGCFAAVLLAIAYGKFSWGLMRQA
                     MIETMRVSAFVVMIAAMCYAFVGIFMNAGAGDVVAEFILSVPGGKWMSFFVIMAIVFL
                     LGMFIEWIGIVFIVVPIFSPILTALGFNPLWAGMMICINMQMAFQTPPMAMSIFVLKG
                     TAPKELGVTIGDIIWGVIPFILIIMFVLLLCAIFPGIITWLPELMIGKA"
BASE COUNT          665 a          793 c          808 g          508 t
ORIGIN      
        1 gctgcgcctc tacgcgaaag agcgcggcat tgcgcatctt gaccttcccg ccgagaagtt
       61 tgtcagccgt atcatggggt tcctgactcc ccgcaattcg gaggtcaccc gtgaattccg
      121 ccgcctcatg gagcaggagg gggccgcggc ggccgtcgcc tggttctacc gcttctccgc
      181 cgacacgatg tacgtcagga tggatcttgt ctcgctggac cggaagtgga cggcgatgac
      241 ggagttcggc gagatgcaga tcaccatcaa tcggtccaag cctgagaaag atccaagaga
      301 tatccgcgcc gcaggagcac aaacgggcga ggatcgttat ccgaaatgtc tgctctgcgc
      361 cgagaatgcg ggctaccagg gacgccccgg acaccccgag cggagcaacc atcgtatcgt
      421 cccgctcgaa ctgtcgggcg aacaatggta catgcagttc tccccctacg tctactacca
      481 tcaccactgt atcgtcttct cgaaggaaca ccggccgatg cgcatcgaca ggatgacgtt
      541 tgtccgcatc tgcgattttc tgacgctttt ccccggcatg ttcatcggat cgaatgccga
      601 cctccccatc gtcggaggtt ctattctcag tcacgatcac tttcagggag gtacgtggac
      661 ttttccgatg acgaacgcgc ctgttctgaa gagtctgcgt gtcgccggcg catccctgga
      721 acatctccgc tggcccttga caactcttcg cctgagaagc cccgacagaa ccgtcctgga
      781 aacgctcggc gagcagctgc tcctctcctg gcgcgagtac gaggacagag agaggggaat
      841 actcgcccga agcggaaacg gagaaagaga ggaacagcac aacaccgtca cggtcatcgg
      901 cagacggcgg ggcgcagagt acgaaataga cgtcgtgctg cggaataatc gctgcgacac
      961 tcagtacccc gacggcatat tccacgcgca cagggaacgc catcatatca aaaaagagaa
     1021 tatcggactc atcgaagtta tggggctcgc gattcttcct ccccgtctgg aatcaagcct
     1081 gaacgacgtg accgcgattc tctcaggaga aaaaactctt tcggatcttc cgcagtactt
     1141 tccccacacc gaatggacag ttgaactggc aacccgtttc ggtaccactc ttcctgcaga
     1201 cgaagcagag cgtatcgtcc ggaacgaggt cggcacagtt ttttccaaag tcctcaggga
     1261 ttgcggagtg ttcaaggcaa ctgaggacgg agaaaaagga gtcgaacgat ttctctcctc
     1321 ctgggcggcg cagtgggggc gttcgtttgc atcagactga agaggccccc aaggagtgtg
     1381 tctccaaaaa gaacggcgac cccgaaaaaa gcgggccgcc gtttgtgttt cggtatgact
     1441 gaggtctcta cgccttgccg atcatcagtt cggggagcca ggtgatgatc cccgggaaga
     1501 tggcgcagag cagaaggacg aacatgatga tgaggatgaa cggaatgacg ccccagatga
     1561 tgtcgccgat cgttactccg agttctttcg gcgccgtccc cttgaggacg aagatcgaca
     1621 tcgccatagg aggcgtctgg aaggccatct gcatgttgat gcagatcatc atgccggccc
     1681 aaagcgggtt gaatcccaaa gctgtcagaa tgggcgagaa aataggcacc acgatgaaga
     1741 cgatgccgat ccactcgatg aacatgccga gaaggaagac gatcgccatg atgacgaaga
     1801 aggacatcca cttccctccc ggtaccgaga ggatgaactc cgccacgacg tcgcccgccc
     1861 ctgcgttcat aaagatgccg acgaaggcat agcacatcgc cgcgatcatc acgacgaaag
     1921 cgcttacgcg catcgtctcg atcatggcct gacgcatcag cccccagctg aatttcccgt
     1981 aggctatggc gaggagaacg gccgcgaaac accctactgc ggcggcttca gtcggtggtg
     2041 caattccggc gaagatagtc ccgagcaccg caatgataag cagtataggc ggcacaaggg
     2101 atttgagaag gcggatcatt tttttggcgt cgaacggcgt tatctgatct tccgggatcg
     2161 cgggaccgag cgccgggttg agcctgcacc ggatcacaac gtagaggatg tagcacgcag
     2221 agagcagcag ccccgggaat acggccccca tgaacatctg cccgaccgag agtccggctt
     2281 gcggcgcata gacgacaagc atgatgctcg gcgggatgag gatgccgagc gttcccgccg
     2341 ctacgatgga tcccgatgcg agtgatttat cataccctct ggtgatcatc ggcgcgaggg
     2401 cgatgagcgt caggatggtc acggaagccg caataacgcc cagacaggcg gcgaggatgg
     2461 taccgaagat gatggtcaca accgcaaggc cgccccggag tcgtcccatc gcctcgtaca
     2521 gcgactggta cagatcttta gtaacccccg agtgttgcag tataaccccc atgaaggtaa
     2581 agaggggaac cgccaggtag gggtagttca acgacaggtc gtaaaaacgg ctgtacagga
     2641 tgtggaacgt caccgttgga ccgaagacag ccagccccgt gaagaacgcc acgctcccga
     2701 tgacgaaagc gatggggaat ccggtcatta caagaacgaa gactcctccc agcatgagaa
     2761 tagtgacggc ttca
//
LOCUS       DAOPKU010000408         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_41673_length_2765_cov_5.101845, whole genome shotgun sequence.
ACCESSION   DAOPKU010000408 DAOPKU010000000
VERSION     DAOPKU010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41673_length_2765_cov_5.101845"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PK849_11450"
     CDS             complement(<1..501)
                     /locus_tag="PK849_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPE66784.1"
                     /translation="MKILVVNCGSSSLKYQLFDMTDESVLAKGLIERIGIEGARIKHT
                     KTGMDAVIKNREIPNHKVAFKVVVEALLDPEHGVLKSLDELAAVGHRVVHAGERYAGS
                     VLITHDVIDALEECVPLAPLHNPANLMGIYAVTEALPNIPQVGVFDTAFHQSMPGYAY
                     MYGVPYH"
     gene            601..1833
                     /locus_tag="PK849_11455"
     CDS             601..1833
                     /locus_tag="PK849_11455"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPE66785.1"
                     /translation="MVAEYNPLHNGHQHHIRRTREKVNPDGIVVVLSSYFTQRGEPAL
                     LSKWDRAESALHAGANLVLELPVFFSSHNAGVFASGAVDVLAATGLVQTISFGMEEPD
                     FDVAPLVDILVQEPITFKDSLKKHLNSGFSYVKARAEALASINAEYGVFVSSPNNSLA
                     LAYMEHIARKGLNIQCMPIQRTGAGYHNTSVTASGEESCFASASAIRRELLESGLDAV
                     AHTLPVPSFEILRRSMETGYYVLSTDLLWRMIRMVLLRTTKKNLSHSSEMTEGMENRL
                     LRYASECGSWDEFLRKCVTHRYPRARIQRQLIHLLLGIDHTENRTFQENGPAYIHPLA
                     ADKKGVEMLRAMRKKASLPIRGKLPLHAQGAEGKLASIECLAAEIWESLTPAFIPGTE
                     KKRHLIRGQEYAEEGNAP"
     gene            complement(1793..2299)
                     /gene="coaD"
                     /locus_tag="PK849_11460"
     CDS             complement(1793..2299)
                     /gene="coaD"
                     /locus_tag="PK849_11460"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPE66786.1"
                     /translation="MKRCISAVYPGSFDPITNGHIYIAERAAALFDEVIVSVLLNPQK
                     KAAFTVDERKAMAREALSHLTNVKVGSFSGLLVDFLRQERSRIIIRGLRALSDFEYEF
                     QLALMNRQLAPDIETLFIVTDARYSYLSSHAVKEIYQFGGAVHEMVPPGVFRRLRERF
                     PLPHTLAP"
     gene            complement(2296..>2765)
                     /locus_tag="PK849_11465"
     CDS             complement(2296..>2765)
                     /locus_tag="PK849_11465"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPE66787.1"
                     /translation="QGRSFLDLFSGTGRVAYRAAQQGASFVAAVEILPVRARDIRSLF
                     PAAPSFLLLAMDIRRALSFLERKGHRFEVIFADPPYGAGWPGILGELLFPKSGGILVP
                     NGVAVIEHSLREPIPSGENYRIIDRREYGETVLSFLASAVLPTDTSGERGEKV"
BASE COUNT          683 a          693 c          732 g          657 t
ORIGIN      
        1 atggtagggg acgccgtaca tataggcata tccgggcatg gactggtgga acgccgtgtc
       61 gaatacgcct acctggggga tatttggaag ggcttctgta acagcgtaaa tgcccatgag
      121 gttggcgggg ttatgaagag gagcaagagg gacgcactct tccagagcat cgatgacgtc
      181 atgggtgata aggaccgagc ctgcgtaccg ctctccggcg tgaacaaccc tgtgcccgac
      241 ggcggcgagt tcatcgaggc tcttcaatac gccatgttcc gggtcaagga gggcctcgac
      301 gacgactttg aaggcaactt tgtgattggg gatttctctg tttttgatta cggcgtccat
      361 tccggtcttc gtgtgcttga ttcgggctcc ttcaatgcct attctctcaa taagcccctt
      421 tgccagcaca gattcgtcgg tcatatcgaa aagctgatac ttgagagatg aactaccgca
      481 gtttacaaca agaatcttca tgaatactac cccttcctct catcttgacc ggacgctttc
      541 gcaggttgta ttgtaataaa caaaaggagg ccggtcgcca tatatggaaa ttatgttggt
      601 attgtagccg agtataatcc attgcataac ggccatcagc atcacatacg ccgaacgcgg
      661 gaaaaagtaa atcccgacgg cattgttgtt gtgctctcat cctatttcac acaaagagga
      721 gagcccgcgc ttctaagtaa gtgggatcgt gcggaaagcg cgcttcatgc cggagctaac
      781 cttgttctgg aactgcctgt tttcttttcc agtcataatg caggcgtctt tgcatcggga
      841 gcggtcgatg ttcttgcagc caccggcctg gttcaaacca tttctttcgg gatggaagaa
      901 cctgattttg atgttgctcc actggttgat attctagtcc aggaaccgat tactttcaag
      961 gattctctga aaaaacatct gaattcggga ttttcctatg taaaagcgcg agcggaagct
     1021 cttgcttcaa tcaatgctga atatggcgtt ttcgtctctt cccctaataa ttcactcgca
     1081 ttggcgtaca tggagcatat tgcgagaaaa gggctgaaca ttcagtgcat gccaattcag
     1141 cggaccggcg ccggttatca caatacttcc gttaccgcat ccggcgaaga atcctgtttt
     1201 gccagcgcct ccgctatacg tcgcgaacta cttgaaagtg gactcgatgc tgtggcgcac
     1261 acgcttccgg tcccaagctt tgaaattctg cggcggtcta tggaaaccgg gtattacgtt
     1321 ctctcgaccg atttgctctg gagaatgata cgtatggttc tgctgcgcac aacgaagaaa
     1381 aatttgagcc attcatcgga gatgacggaa gggatggaaa acaggttgct tcgttatgcc
     1441 tcagaatgcg gctcatggga tgaatttttg aggaaatgcg tgactcatcg atacccgcga
     1501 gccagaatcc aacggcagct cattcatctt ctgctgggta ttgatcacac cgaaaaccgg
     1561 acttttcagg aaaacggtcc tgcgtatatt cacccgcttg cagcggataa aaagggagtg
     1621 gagatgcttc gggcaatgcg gaagaaagca tcgcttccca tacgagggaa gctgcccctt
     1681 cacgcgcagg gagccgaagg aaagttggct tctattgaat gtttggccgc agaaatctgg
     1741 gaaagtctca cccctgcctt cattcccggg actgaaaaaa aaaggcatct catcaggggg
     1801 caagagtatg cggaagaggg aaacgctccc tgagacgccg gaagaccccc ggcggaacca
     1861 tttcgtggac ggctcctccg aactgatata tctcctttac agcatggctt gaaagatatg
     1921 agtaccttgc gtcggttacg atgaacaacg tttctatatc gggggctaat tgtctgttca
     1981 tcagagccag ctgaaactcg tactcgaaat ccgagagcgc ccggagcccc ctgatgatga
     2041 tacggctccg ttcctgtcgc agaaaatcaa cgagaagacc ggagaaggaa ccgactttga
     2101 cgttggtcag atggctcaac gcctctctgg ccatggcttt cctttcgtcg acggtaaacg
     2161 ccgccttttt ctgaggattc aagagaacgc ttacaatgac ttcatcgaag agcgccgctg
     2221 ctctctccgc gatgtagatg tgtccgttgg tgataggatc gaacgacccg gggtagactg
     2281 cgcttataca tctcttcata ctttttctcc tctctctccg gaagtatctg tgggcaaaac
     2341 ggccgatgca agaaaagaaa ggaccgtctc tccgtactcg cggcgatcga tgatcctgta
     2401 gttttcgccg gaagggatgg gctcccgaag ggaatgctct attaccgcaa ctccgttcgg
     2461 aacgagaata cctcccgatt tcggaaaaag cagttccccg aggatgccgg gccaccccgc
     2521 gccatacggc gggtcggcaa agattacttc aaaacgatga cctttccgtt ccaggaaaga
     2581 aagcgctctt cgaatatcca ttgcgaggag aagaaaagag ggggcggcgg gaaaaagaga
     2641 gcgaatatcc cgggcacgaa cgggcaatat ctccaccgct gcaacaaaag aagctccctg
     2701 ttgggctgcg cgatacgcaa ccctgccggt tccggagaaa aggtcgagga aagagcgacc
     2761 ttgca
//
LOCUS       DAOPKU010000409         2757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_41861_length_2757_cov_3.599186, whole genome shotgun sequence.
ACCESSION   DAOPKU010000409 DAOPKU010000000
VERSION     DAOPKU010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41861_length_2757_cov_3.599186"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PK849_11470"
     CDS             <1..936
                     /locus_tag="PK849_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66788.1"
                     /translation="TRTKVILPVVLSKVGVTSTETEMILSDFHRCLEAYTEVQMRTRK
                     YREAEEKERQQMAVQNHAFIEAEKRIAGALKGNEEDEGLDENEIAKVTKLLESAKTLA
                     RNIVYVEQELVKWDRKVKKKAADLTAEAPAFRRRELRNMIEAKKEYISLTAKSARLDD
                     SQLCQSDKLPFSIDKAAAILEDMIALDPEMLVVARVRMYGIPRVILVPGQGYGTYDWN
                     DHTLLIPAFPTYSQERAAAYALGTFRWDSDEDRVIKNSYELIKENRDKSILDLNTSFY
                     KDYFLWLTKEKKGYRILPRSTHKVFVQMFAPQKQE"
     gene            961..1233
                     /locus_tag="PK849_11475"
     CDS             961..1233
                     /locus_tag="PK849_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66789.1"
                     /translation="MFERIISILLGSAILYLVRFKLLRKPLSPIRSILVATVIMVAGI
                     QFHYAYLPYETFQGGVTYTSLGFIAFLMFQYGYRKTAPSKKESAEK"
     gene            1274..2077
                     /locus_tag="PK849_11480"
     CDS             1274..2077
                     /locus_tag="PK849_11480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPE66790.1"
                     /translation="MERDEAMSASAVAENFSATLRLWGALPTSRAEARGPCLFVSTGT
                     AIPDQNYAYCPVEEDAEENVRSSARFFREERMPFTWWFPPGTRGERFRRALEEQRMPE
                     RCAPPAMLMALDAPAERAHHSAERTVCKAVSSAEAEEWACSSLEGFDSGPEHQGPFSA
                     FCTSLLVPPFDEAFRLLTLRVGGRAAATALLSLTGEVAGIYYFSTLPAFRRRGLGRLL
                     LSEALEEARRSDCCTAVLQASSMGFPLYKKAGFAVCGHFAVHSADPDAC"
     gene            complement(2074..>2757)
                     /locus_tag="PK849_11485"
     CDS             complement(2074..>2757)
                     /locus_tag="PK849_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPE66791.1"
                     /translation="ALVEYLGHGSHPASFILLLFVGKLLFTALCFGSGAAGGIFLPML
                     AVGASLGAFYGTVAVQVYGLDQALVLNFLIVGMAGFFSAVVRAPITGIVLITEMTGSF
                     THLLSVSAVAVTAYVVADLLRARPIYDSLLLRMLRKCDTCEESGKVLLEGTVSPESPI
                     DGKRVRDARLPAGCLLVSIDRAGEEILPNGETVIAAGDKIVALVDGRRARRVRETLHD
                     LLEVRRKTT"
BASE COUNT          611 a          848 c          825 g          473 t
ORIGIN      
        1 acaaggacga aggtcatcct tcccgtggtg ctctccaaag tcggcgtcac atcgacggag
       61 acggagatga tactctccga tttccaccgc tgtcttgaag cgtataccga ggtacagatg
      121 cgcaccagga agtacaggga ggccgaggag aaggagcgcc agcagatggc cgtgcagaat
      181 cacgctttca tcgaagccga gaagcgcatc gcgggcgccc tgaaagggaa cgaggaagat
      241 gaggggcttg acgaaaacga gatcgccaag gtcacgaagc tgcttgagag cgcgaagacg
      301 ctcgcccgga acattgtcta cgtggaacag gaactcgtta aatgggaccg gaaagtcaaa
      361 aagaaggcgg ccgatctcac ggcggaagcc ccggcgttcc ggcgcaggga actccggaac
      421 atgatcgagg cgaagaaaga gtacatctcg cttacggcga agtccgcccg gctggatgac
      481 tcacagctct gccagagcga caagcttcct ttttccatcg acaaggccgc ggcaatcctg
      541 gaggatatga tcgcgctcga cccggagatg ctcgtcgtgg cacgagttcg catgtacggc
      601 ataccgcgcg tgatcctcgt ccccgggcag gggtacggca cgtacgactg gaacgaccac
      661 accctgctga tcccggcgtt tccgacctac agccaggagc gcgccgcagc ctacgccctg
      721 ggaacgttcc gctgggacag cgacgaggac cgcgtcatca agaacagcta cgagctcatc
      781 aaggagaacc gggacaagtc catcctcgac ctgaacacgt cgttctacaa ggactatttc
      841 ctctggctga cgaaagagaa gaagggctac cgcatcctgc cgcgcagcac gcacaaggtc
      901 ttcgtacaga tgttcgcgcc tcagaaacag gaatgaaaaa cagagtaaaa ggagagtcca
      961 atgtttgaac gaatcattag tatacttctt ggcagcgcca tcctctatct tgtgcgcttc
     1021 aagctgctgc ggaaaccgct ctcgccgatt cgatccattc tcgtcgcgac ggtcatcatg
     1081 gtcgccggaa tacaatttca ctacgcctat ctcccctacg agacgtttca agggggcgtc
     1141 acctacacgt cgcttggctt cattgcgttc ctgatgttcc agtacggcta ccgcaaaacc
     1201 gctccctcca agaaagagag cgcagaaaag tagcccgtcc acgctgcaga cttcataaaa
     1261 aggcggagcg gcaatggaga gagacgaagc gatgagtgca tctgctgttg cggaaaactt
     1321 cagcgcgacg ctgcgactct ggggggcgct gccgacgtcg agagccgagg cgcgcggccc
     1381 ctgtctcttc gtgtcgaccg gaacggccat accggaccag aactacgcgt actgccccgt
     1441 ggaagaggac gccgaggaga acgtacggag ttcagcgcgt ttttttcgcg aggagaggat
     1501 gcccttcacg tggtggttcc cccccggaac acggggagaa cgcttccgga gagcgcttga
     1561 ggagcagcga atgcccgagc ggtgcgcgcc gccggcgatg ctcatggcgc tcgacgcacc
     1621 ggcggaacgc gcacaccatt ctgcggagcg caccgtttgc aaggctgtct catccgctga
     1681 ggccgaggaa tgggcctgtt cctcccttga aggattcgac tcggggccgg agcatcaagg
     1741 acctttctcc gcattctgca cgtcgcttct cgtaccgcct ttcgacgaag cgttcagatt
     1801 gctgacgctt cgcgtggggg ggcgcgctgc ggcaaccgcc cttctctctc tgaccggaga
     1861 ggtggcagga atctactact tctccactct ccccgccttc cgcaggcgag ggctggggag
     1921 gctcctgctg tcggaagcgc tggaggaggc gcggcgctcc gactgctgta cagccgtgct
     1981 ccaggcctcc tcaatgggat ttcctctcta taaaaaagca ggcttcgccg tgtgcggcca
     2041 ttttgcggtc cactccgccg atcccgacgc ctgctaagtc gtttttcggc gaacctccag
     2101 aagatcatgc agcgtctcgc ggacgcgacg cgctctcctc ccgtcgacaa gcgccacgat
     2161 cttgtcgccc gccgcgatca cggtctcgcc gttcggaaga atctcctccc ccgcccggtc
     2221 gatgctcacg agcaggcacc ccgccggaag ccgtgcatcc cgaacgcgct tcccgtcaat
     2281 gggcgactcg ggagagaccg taccttcgag cagcaccttt ccggactcct cgcacgtatc
     2341 gcacttccgc agcatgcgca gaagcagcga atcatagatc ggacgcgcgc gcagcaggtc
     2401 cgcgacgacg taggccgtca ccgccaccgc ggagacggag agcagatggg taaacgatcc
     2461 ggtcatttcg gtgatgagga caatccctgt gatcggggcg cggactacag ccgagaaaaa
     2521 accggccatg ccgacgatga gaaagttgag caccagcgcc tgatcgaggc cgtagacctg
     2581 gacagcaacg gtcccgtaga atgcgcccag cgacgcgccg acagcgagca tggggagaaa
     2641 gatgcctccc gccgctcccg agccgaaaca gagtgcggtg aagaggagtt tccctacaaa
     2701 gagcaacaga atgaagctcg caggatgact cccgtggccg agatactcca cgagcgc
//
LOCUS       DAOPKU010000410         2756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_41878_length_2756_cov_4.256201, whole genome shotgun sequence.
ACCESSION   DAOPKU010000410 DAOPKU010000000
VERSION     DAOPKU010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41878_length_2756_cov_4.256201"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1037)
                     /locus_tag="PK849_11490"
     CDS             complement(108..1037)
                     /locus_tag="PK849_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HPE66792.1"
                     /translation="MSNLRAAVFAYSEVGHTCLKELLRQGTSVQAVFTHEDNPAETLW
                     FPSVAALAREAGIPVFTPDSLGDEEYAMLLSLRPDVIFSFYYRSMIPERFLAIPPKGA
                     FNMHGSLLPKYRGRACVNWAVLNGETVTGATLHHMVRRADAGDIVDREAVPILPDDTA
                     LDVSRKVAEAAGIILERTLPSIALGAAPRTPQNEGEATYFGRRTPADGLIDWRESAVR
                     IHNLVRAVTHPFPGAFTFFRGKKLFIWKTQTGEHPSFDTPPGTVLSEDPLRVVTGRGV
                     LRLLEWQFKDSDAQSPGAGDILGKEPEGETRPS"
     gene            complement(1030..1968)
                     /locus_tag="PK849_11495"
     CDS             complement(1030..1968)
                     /locus_tag="PK849_11495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE66793.1"
                     /translation="MSESCVLSVIVPVYNEEESLPYLCPRLLKTLDGMHRPYEIIFVN
                     DGSRDASLKMLLDFHKANPGKVRVVDFNGNFGQHMAIMAGFDHSRGDVIVTLDADLQN
                     PPEEIPNLVAKIDEGHDVVGTIRQNRQDPKFRKMASRMVNAITNKITGLRLNDYGCML
                     RAYRRDIVNIINQSCESTTFIPALAQKFAVNPVEIPVGHTEREHGTSKYSIFRLIRLN
                     FDLMTGFSLFPLQAVTMIGIVVSALSFVFALYLLLRRIIVGPEAEGVFTLLNINFFLM
                     GITMSCVGITGEYIGRIYQEVRKRPRYVIRREWNDE"
     gene            complement(2067..>2756)
                     /locus_tag="PK849_11500"
     CDS             complement(2067..>2756)
                     /locus_tag="PK849_11500"
                     /inference="COORDINATES: protein motif:HMM:NF037277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66794.1"
                     /translation="VPAAPPLAILMGRYLSRCIEEEDVKKTGRFLLWNSLLLFPFIGA
                     LLVYPFFNDRIAAGRLLPYSLPIAAVLILFVFSGWHSFLRRDFRRLALFLCVLSFANM
                     FAFSRVFLLYDSLLTARELAGRIAEIRNDGDIVAQFANYDQGLPFYLKQRIVLVNYMG
                     ELEFGALQETDPSWFIDGKGLAELWTGDRRVILVVNSDHKEYVAQQTGLSLPEPLAET
                     AKRLVFVNRKE"
BASE COUNT          535 a          795 c          898 g          528 t
ORIGIN      
        1 aagccgttgg ccccgagaat cagaatgttc atagagaaac ctccctgcgt gttccccgcg
       61 ttgtgcggga tggttcgttc gtggaaaaaa gtcgccacga gagtatatca cgaagggcgc
      121 gtttcgccct ccggctcttt gcccagaata tctcccgcgc ccggtgattg tgcatcggag
      181 tccttgaact gccattcgag gagacgaaga acgccccgtc ctgtgacgac gcggagcgga
      241 tcctccgaga gtaccgttcc cggaggcgta tcgaaagacg gatgctcccc ggtctgcgtc
      301 ttccagatga aaagcttctt tccacgaaag aacgtgaagg cgcctggaaa gggatgcgtc
      361 accgcgcgga ccaggttgtg aatccggacg gcgctctcac gccaatcgat caggccgtcc
      421 gcgggcgtcc tgcggccgaa gtatgtcgcc tctccctcgt tttgcggggt ccgcggcgcg
      481 gcgccgaggg cgattgaggg gagcgtccgc tccaggatga tccctgcggc ttcggccact
      541 ttccgggaga cgtcgagggc cgtatcgtcg gggaggatgg gcacagcctc ccggtcgacg
      601 atatcccccg cgtcggctcg tcgtaccatg tggtggagcg tcgcgccggt taccgtctcg
      661 ccgttgagga cggcccagtt gacgcaggcc cgtcccctgt acttcggcag cagcgagccg
      721 tgcatgttga aggccccctt cggcgggatc gcgaggaagc gctcggggat catcgagcgg
      781 tagtagaaag agaagatgac gtcggggcgc agagagagca gcatcgcgta ctcctcgtcg
      841 ccgagagagt ccggggtgaa gacggggatt cccgcttccc gcgcgagcgc cgccaccgag
      901 gggaaccaga gcgtttccgc cgggttgtct tcgtgggtga agacggcctg aaccgacgtc
      961 ccctgacgca gcagctcctt caggcatgtg tgcccgacct cgctgtaggc gaagacggcg
     1021 gcgcggaggt tactcatcgt tccactccct gcggatgacg tagcgggggc gtttgcggac
     1081 ctcctggtag atgcgtccga tatactctcc cgtgatgccg acgcaggaca tggtgatgcc
     1141 catcaggaaa aagttgatgt tgagcagcgt gaagacgccc tccgcttccg ggccgacgat
     1201 gattctgcgg agcagcaggt agagtgcgaa gacgaagctc agcgcggaga cgacgatgcc
     1261 gatcatcgtc accgcctgaa gcgggaagag agagaacccc gtcatcaggt cgaagttgag
     1321 ccggatgagg cggaagatgc tgtacttcga cgtgccgtgc tcccgctccg tatgccccac
     1381 agggatttcg accgggttga cggcgaactt ctgcgccagc gcgggaatga aggtcgtgct
     1441 ctcgcagctc tggttgatga tgttgacaat atctcgccgg taggcgcgga gcatgcagcc
     1501 gtagtcgttc aggcgaagcc ccgtgatctt gttggtgatg gcgttgacca tgcgcgaagc
     1561 catcttgcgg aacttcgggt cctggcggtt ttggcggatc gttcccacga cgtcgtgccc
     1621 ctcgtcgatc ttcgccacaa gatttggtat ctcttcgggc gggttctgga ggtcggcgtc
     1681 gagcgtcacg atgacgtctc cccgcgaatg atcgaatccg gccatgatcg ccatgtgctg
     1741 gccgaagttg ccgttgaagt cgacgacgcg caccttcccc ggattggcct tgtgaaagtc
     1801 gagcagcatc ttcagggagg cgtccctgct tccgtcgttg acgaagatga tctcgtaggg
     1861 acgatgcatt ccatcgagcg tcttcagcag cctcgggcag agatagggga gagactcctc
     1921 ctcgttgtag accggtacga tgacagagag aacgcatgat tcggacattg ttatttccct
     1981 tctccttccg ggatggttgc tcatgaagag caaatactcc cacccgggcg ccgcccgggt
     2041 gctaccatgt accgcgccgg tgcgggttat tccttgcggt tgacgaagac gaggcgcttt
     2101 gcagtctcgg ctaaaggttc cggaagcgaa agcccagtct gctgcgcgac atactccttg
     2161 tggtcgctgt tgacgaccag gatgacgcgc cggtcgcccg tccaaagctc ggcgagcccc
     2221 ttcccgtcga tgaaccagga gggatcggtc tcctgaagcg ccccgaactc gagttctccc
     2281 atgtagttga ccagcacgat ccgctgtttc agatagaagg ggagcccctg gtcgtagttt
     2341 gcgaactggg cgacgatgtc gccgtcgttt cggatttcgg cgattcttcc cgcgagttcg
     2401 cgcgcagtaa gcaaggaatc gtagagaagg aagaccctgc tgaaggcgaa catgttcgcg
     2461 aaggagagca cgcagaggaa gagggcaagt ctcctgaaat ccctgcggag gaagctgtgc
     2521 cacccggaga agacaaacag gatcaggacc gctgcgatcg ggagcgagta ggggagcaga
     2581 cgtccggcgg cgatccggtc gttgaagaac ggatagacaa gcagcgcgcc gataaaggga
     2641 aacagcaaca gcgaattcca cagcaggaag cgccccgtct tcttgacgtc ctcctcttcg
     2701 atgcagcggg agagataccg ccccatcaga atcgcgagcg gcggcgctgc ggggac
//
LOCUS       DAOPKU010000411         2750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_42026_length_2750_cov_3.897588, whole genome shotgun sequence.
ACCESSION   DAOPKU010000411 DAOPKU010000000
VERSION     DAOPKU010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42026_length_2750_cov_3.897588"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PK849_11505"
     CDS             complement(<1..717)
                     /locus_tag="PK849_11505"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: protein motif:HMM:NF013403.2"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HPE66795.1"
                     /translation="MNYTVMQFGEGNFIRSFFDWMLKRITDATGEEHKVFLVQPISNG
                     RVKEIVEAGEYHVLLKGFEAGEYKEVLESVPVIAGGVNPFERGGMKALLDAALSPELR
                     VVTSNTTEAGIFFEEKMEPHNFPSLLAAVLRARAKEGAPPLHILPLELIESNGTALKK
                     CVEQYGRLWGCEPVFFSYLDSCTFYDTLVDRIVPGFPAKDAPEIFRRLGKEDHNLAMG
                     ELFHLFVIQGDKAILDVLPFH"
     gene            complement(793..2283)
                     /locus_tag="PK849_11510"
     CDS             complement(793..2283)
                     /locus_tag="PK849_11510"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HPE66796.1"
                     /translation="MECTHDGARCIVLRENDSVAVLPEGGGAGSRLPFAEQELVLKEV
                     IAPGHKIALRDIKQGERVVKYGFPIGVASRPIAAGEHVHVQNVRSALGHEWSPSWSPS
                     PARLLSPLSPVPSFQGYRRTEGPPGIRNELWIIPTVGCVNEYLKFLAGAWTAPSWIEN
                     VRVLSHPYGCSQLGEDLERTAAVLAGLARNPCAAGVIVAGLGCENLQRVFLETRLAGV
                     EKVRFLSLQEEDDDAGALYALLDELAADAPKEREPFPLSGVTLGVKCGGSDGFSGLSA
                     NPLVGEVADRMSAWGGKILATEIPEMFGAEDVIASRIADRQVFDAFAETIRWFRNYYV
                     EHKQPVYENPSPGNKDGGITTLEEKSLGAVNKCGSGAVTDVLAMGEQAKRPGVSVVFG
                     PGNDLVSSTALAAGGAQIILFTTGRGTPFSTVVPTIKISSNTRLFGRKPRWIDFDAGP
                     LLSGTPRHRLAEDLTGLVLEVASGRRTRNEENRSGEIAIFKDGVTL"
     gene            complement(2478..>2750)
                     /locus_tag="PK849_11515"
     CDS             complement(2478..>2750)
                     /locus_tag="PK849_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPE66797.1"
                     /translation="FDLVERAVRLSFESLNEEGVIKTKEVDALEDEVDRKEKILRKNH
                     ILRLNKGECTPAGGVIFIDILSNLERVCDHAHNISLIAFDISKLHR"
BASE COUNT          577 a          862 c          760 g          551 t
ORIGIN      
        1 gtggaagggc aggacatcga gaatagcctt gtccccctgg atcacgaaca gatgaaagag
       61 ctcccccatg gcgaggttgt gatcctcttt ccccagacgc cggaagatct ctggagcgtc
      121 cttggccggg aagcccggga cgatacgatc caccagcgta tcgtagaagg tacaggagtc
      181 gagataggag aagaataccg gttcgcaccc ccagagacgt ccgtactgct ccacgcactt
      241 tttcaaagcc gttccgttac tctcgataag ctccaacgga aggatatgca gcggaggcgc
      301 cccctccttt gcgcgggcgc gcagaactgc ggccagcagc gacggaaagt tgtgcggctc
      361 catcttctct tcaaagaaga tcccggcctc ggtggtattc gacgtcacca cccgaagctc
      421 cggagaaagc gcggcgtcga gcaacgcttt cattccgccg cgttcgaagg ggttgacgcc
      481 ccccgcgatg accgggacgg actcaagaac ctctttatac tcccccgcct cgaatccttt
      541 cagaagaacg tggtattctc ccgcctcgac gatctctttc actcttccgt tggaaatagg
      601 ctgcacaaga aagaccttgt gctcttcgcc cgtcgcgtcg gtaatgcgct tgagcatcca
      661 gtcgaaaaac gaccgtatga agttcccctc gccgaactgc atcactgtat agttcatcgt
      721 atcctctcct tcatcgcgag catttctgtg catcttggtt tcgcacggcg tcctcgttga
      781 aatcggggcc tcttagagcg tcacgccgtc tttgaatatg gcgatctccc cggagcggtt
      841 ctcctcgttt cgggtacggc gccccgacgc aacttcaagc acaagacccg tcaagtcttc
      901 agcgagacgg tgtctcggcg tcccggagag cagcggcccg gcgtcgaagt cgatccagcg
      961 cggtttccgc ccgaaaaggc gcgtgttcga ggaaatcttt atcgtcggaa ctaccgttga
     1021 gaacggcgtc ccgcgtcccg tagtgaaaag gatgatctgt gcgcccccgg cggccagagc
     1081 agtcgacgac acaaggtcgt ttcccgggcc gaacacgacg ctcacaccgg ggcgcttcgc
     1141 ctgttcgccc atggcgagga cgtctgttac cgcaccggaa ccgcacttgt tcaccgcgcc
     1201 gagcgacttc tcctccaacg tagtgatgcc gccgtccttg ttccccggag acgggttctc
     1261 gtacaccggc tgcttgtgtt ccacatagta gttccggaac cagcgaatgg tctccgcaaa
     1321 ggcatcgaag acctgcctgt cggctattcg agaggcgata acatcctcgg cgccgaacat
     1381 ctcaggtatt tcggtggcga ggatcttccc cccccacgca ctcattctgt cggcgacttc
     1441 gccgacaaga ggattggcgg acagaccgga aaaaccgtcg ctgcccccgc acttcacacc
     1501 gagcgtcacc ccggaaaggg ggaatggttc tctctctttc ggagcatccg ccgcaagctc
     1561 atcgaggaga gcgtacaagg ctccggcatc gtcgtcctcc tcctgaagcg agaggaatcg
     1621 gactttctcc actcctgcaa ggcgggtctc caaaaagacg cgctggaggt tttcacaccc
     1681 gagccccgcc acaatcaccc cggccgcgca cggattgcgc gcaagtcccg cgaggactgc
     1741 tgccgtccgt tccaaatcct ccccgagctg ggagcagccg taggggtggg aaagcacgcg
     1801 gacgttttcg atccaggaag gggccgtcca cgctccggcg aggaatttca ggtactcgtt
     1861 gacgcacccg accgtcggaa tgatccagag ctcgttccgt atgcccggag gcccctccgt
     1921 tcttcggtat ccttgaaatg aaggaacggg cgatagcggg gagagaagac gcgcaggaga
     1981 agggctccac gacggcgacc attcgtgtcc gagagcggag cggacgttct gcacatgcac
     2041 atgctcgcct gcggcgatcg gacggctcgc aacgccgatc ggaaaaccgt acttcaccac
     2101 ccgctccccc tgcttgatgt cccggagagc aatcttgtgt ccgggagcga taacctcttt
     2161 aagaacgagc tcctgctcgg caaagggaag ccgcgatccg gcgcctcctc cctcgggaag
     2221 aaccgcgacg ctgtcgttct cccggaggac gatacagcgc gctccgtcgt gtgtgcattc
     2281 catagcatct cctccttgtt ttatccatta aaacaaaaat ttaccgagta aaacaagaac
     2341 cgcctacaga atagcgaatg cacccggtat gtcaagcggg gagcatgaaa aaactcgttc
     2401 aaaaaaccgg cgaggagcct ccccgccggt ttcaagacat accaatattc gtccacctca
     2461 agaagcccgg tcgtccgtca tctgtgcagc tttgatatat cgaacgcgat gagcgagata
     2521 ttgtgggcgt gatcgcacac tcgttcgaga ttgctcagga tgtcgatgaa gatgacgccc
     2581 ccggcgggag tgcactcccc tttgttgaga cggaggatat ggtttttccg gagaattttc
     2641 tccttgcggt ccacctcgtc ctcaagggcg tcgacctcct tggtcttgat gacgccctcc
     2701 tcgttgagcg actcgaaact cagacgaacc gcccgttcaa cgaggtcgaa
//
LOCUS       DAOPKU010000412         2750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_42027_length_2750_cov_3.774397, whole genome shotgun sequence.
ACCESSION   DAOPKU010000412 DAOPKU010000000
VERSION     DAOPKU010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42027_length_2750_cov_3.774397"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PK849_11520"
     CDS             complement(<1..891)
                     /locus_tag="PK849_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPE66798.1"
                     /translation="MARIEYVNIEDALGMALSHDMTQIDVKSGYKGARFKRGHIVKPE
                     DLPVLRSMGKNSISVLYLEEGDVHEDDAALRLAHRLAGEGLRMEGPEEGKVSLFAEWN
                     GLIVYDEESIHTINEDVDWIAVTTPNKVPVKKGEPAAGIRVVPLTMREEQIRKAEAAA
                     LPLTVFPFHPLKTALVTTGREIVEGRIRDAFAPKLVRKLAFYGSTLMEQVITGDEKDE
                     IARAIQSFIDKGAELIIVTGGMSVDADDRTGAAIVQVSDSVVFNGVPALPGARLMLAL
                     QDKTKIIGAPACVVHDEWTSM"
     gene            1083..1553
                     /gene="nuoE"
                     /locus_tag="PK849_11525"
     CDS             1083..1553
                     /gene="nuoE"
                     /locus_tag="PK849_11525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPE66799.1"
                     /translation="MAFSVEEIREKTRKITEPWIERNGGLIPILQAVQAQLGYLPQEA
                     LETISRELKIPPSEIYGVATFYAQFHLKPRGRHVVRVCRGTACHVRGSLKILEKVKNL
                     IGVGENETTEDLRFTIEPVACLGACGLAPVMMVDTQTFGRLVPDMVKGILDKFE"
     gene            1570..1944
                     /locus_tag="PK849_11530"
     CDS             1570..1944
                     /locus_tag="PK849_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPE66800.1"
                     /translation="MEQKKITSLDDLRKIRENSKDLSSARSGGGTTVIVGMGTCGIAA
                     GAREILNEVMSELARRNLDDIAVQTTGCIGMCQDEPLLDVIRSGQRVTYGRVKPEDVP
                     TIIAEHVVNGRVVEKLAIGRAD"
     gene            1963..>2750
                     /locus_tag="PK849_11535"
     CDS             1963..>2750
                     /locus_tag="PK849_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPE66801.1"
                     /translation="MTIFRSHVLICGGTGCTSSGAAEVIGSFKQELARRKLDGEVLVV
                     PTGCHGMCEMGPIVVVYPEGTFYCKVTANDVPEIVEEHIYKGRPVQRLMYEGSGDMPA
                     LPHYKDIPFYGKQHRIALANCGYINPENIDEYIARGGYEALGKAITSMTPTQVIDEMK
                     TSGLRGRGGGGFPTGLKWSFCAAAGGEKKYVICNADEGDPGAFMDRSILEGDPHSVIE
                     GMMLGAYAMGADEGYIYCRAEYPLAIERLKQAITAAEGYGLLVSG"
BASE COUNT          600 a          711 c          832 g          607 t
ORIGIN      
        1 catgctggtc cattcgtcat gcacaacgca tgccggagct ccgataatct tcgtcttgtc
       61 ctgaagcgca agcatcaggc gggcgcccgg aagtgccgga acgccgttga acaccacgct
      121 gtcggaaact tggactatgg ccgccccggt ccggtcgtcc gcgtcgacgc tcatgcctcc
      181 ggtgacgatg atcagctcgg cccccttgtc gatgaaggac tgaatggcac gggcgatttc
      241 gtccttttcg tcgcctgtaa tgacctgttc catcagggtc gatccgtaga acgccagctt
      301 gcgcaccagc ttcggcgcga aagcgtcgcg gatgcgccct tcgacgatct cgcgtcctgt
      361 ggtgacaagc gctgttttca gagggtggaa cggaaagact gtgagcggaa gcgcagctgc
      421 ttccgccttg cggatctgct cttcgcgcat cgtcaacgga acgacgcgga taccggcggc
      481 gggttctccc tttttgacgg gaaccttgtt cggcgttgta acggcaatcc agtcgacatc
      541 ctcgttgatt gtgtgaatgg actcttcgtc gtacacgatc agtccgttcc attcggcgaa
      601 gagcgagact tttccctcct cgggaccctc catacggagg ccctcgcctg caaggcggtg
      661 tgcaagcctg agggcggcgt cgtcttcgtg aacgtcgcct tcctccaggt agaggaccga
      721 aatggaattc ttccccatgc tcctgagcac cggaagatct tcaggtttga caatgtgtcc
      781 tctcttgaag cgggcgccct tgtaaccgga cttgacatcg atctgggtca tgtcgtgcga
      841 aagagccatt cccaacgcat cttcaatgtt tacgtactct attctggcca ttctttcccc
      901 tcgctcttta taaaacatct ttacgatgaa aaccgcggct attatagcat gtcggggaga
      961 tgtatcggca atataaagta tacacttttc aattacttgt ttttggttcc tgcctatgat
     1021 atgatatttt tcattgtgat attgtgcaca atcctatgtt ctgttaaaaa aggaggggaa
     1081 tcatggcttt cagcgttgaa gaaatccgcg agaagacacg aaagattaca gaaccctgga
     1141 tagagaggaa cggcggcctt atcccgattt tacaggctgt tcaggcgcag cttgggtatt
     1201 tgccacaaga ggcgttggag actatttccc gagagttgaa gatcccgccg tctgaaattt
     1261 acggcgttgc gacgttttac gcccaatttc acctgaagcc tcggggacga catgttgtgc
     1321 gcgtctgtag ggggacggct tgccacgttc ggggaagtct caaaattctc gagaaggtga
     1381 agaatcttat cggggttggc gagaacgaga ccactgagga tctccggttt actatagagc
     1441 ctgtggcctg tctcggcgca tgcggtcttg ccccggtgat gatggtcgat acgcagacat
     1501 tcggaaggct cgttcccgac atggtgaagg gcattcttga caagttcgag tagaacgggg
     1561 gaagagcgaa tggagcagaa aaagatcacg agtctcgatg accttcggaa gatacgcgaa
     1621 aactcgaaag acctctcctc ggcgcgttcg ggtggcggaa cgaccgttat tgtcggtatg
     1681 ggaacctgcg gtatcgcggc aggagctcgg gagatactca acgaggtgat gtcggagctt
     1741 gccaggcgta acctcgatga tattgcggtg cagacgacgg ggtgtatcgg catgtgccag
     1801 gacgaacccc tgctcgatgt gatccgttca gggcagagag tgacgtacgg ccgcgtgaaa
     1861 ccggaggatg tcccgacgat catcgcggaa cacgtcgtga acggacgcgt cgtggagaag
     1921 ctggccatcg ggcgggccga ctgagcggaa ggagaaggac gaatgacaat tttccgatcc
     1981 catgttctta tctgcggcgg taccggatgt acttccagcg gcgccgcgga ggtcatcggc
     2041 tcctttaaac aggagcttgc gcggcggaag ctcgacggag aggtcctggt cgtcccgacc
     2101 ggatgtcacg gcatgtgcga gatggggccc atcgttgtcg tgtaccctga aggaacgttc
     2161 tactgcaagg taacggcgaa cgatgttccg gagatcgtcg aagaacacat ctacaaagga
     2221 cgtccggtgc agcgcctcat gtacgagggt tcgggcgata tgcccgcatt gccgcattac
     2281 aaggatatac ctttctacgg gaagcagcac aggatagccc ttgcgaactg cggctatatc
     2341 aatccggaga atatcgacga atatatcgcc aggggagggt acgaggccct cggcaaggcg
     2401 atcacctcga tgactccgac gcaggtaatt gatgaaatga aaacctccgg cctccgcgga
     2461 agagggggcg ggggattccc cacagggttg aaatggagtt tctgcgccgc tgccggcggg
     2521 gagaaaaaat atgttatctg taacgccgac gagggggacc ccggcgcgtt tatggaccgt
     2581 tccatacttg agggagaccc gcacagcgtg atcgagggta tgatgctcgg cgcctatgcg
     2641 atgggggccg acgaggggta catctactgc cgggcggaat acccgctggc aatagaacgc
     2701 ctgaaacaag cgatcactgc ggccgagggg tacggactgc ttgtttcagg
//
LOCUS       DAOPKU010000413         2740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_42261_length_2740_cov_5.277467, whole genome shotgun sequence.
ACCESSION   DAOPKU010000413 DAOPKU010000000
VERSION     DAOPKU010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42261_length_2740_cov_5.277467"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PK849_11540"
     CDS             complement(<1..772)
                     /locus_tag="PK849_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HPE66802.1"
                     /translation="MAHFFIFYTRLRTEEKLLFKAAEERGIPFSAVNVSDGMWPDIPG
                     ASGDVALCRCVSQTQNSALAVLLEARGVLTVNSSEVMALCADKIVTAAKLDAAGIPQP
                     EYAVAFSPEGAIEGAKRLGYPLVIKPATGSWGRLLAKINDRDALEAVAEHKAHMGAAH
                     SVLFMQQYVEKKGFDLRATLLGGEPITVIKRCSEHWITNTARGATPEKYPLSPEIKAL
                     LGRVQDAIGGEVLAVDLFETAEGGWMVNEVNGQPEFRSS"
     gene            complement(773..946)
                     /gene="lysW"
                     /locus_tag="PK849_11545"
     CDS             complement(773..946)
                     /gene="lysW"
                     /locus_tag="PK849_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HPE66803.1"
                     /translation="MSTKVQCVVCEGDVMLPAEVMEGELLTCPDCGTELEVVSLNPLT
                     VAEAPEVQEDWGE"
     gene            1260..2063
                     /locus_tag="PK849_11550"
     CDS             1260..2063
                     /locus_tag="PK849_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE66804.1"
                     /translation="MRRMSLLFLCVAACMLSLGSVARAVEMRIAYEDNEQPPYIYGNS
                     AAIPEKPGLSVEMILMLPEKIPGIEIKLVRMPWLRCIVELENNEVDGIFNASYKKERL
                     RIGRYPTVDGTPDGPVDVDSRIAGMTYSFYVLRKNVGSFQWDGVHFDTIRNAGTIGTP
                     LGYSIAGDLREQGVAVDESGGPETNLAKLAGGRFVAAALLEATVEGLIRSNALYSEIV
                     KVNPPLETKPYYLMLSRAFVEEHPELSRRIWGALREIRDTKTAEILTRY"
     gene            complement(2132..>2740)
                     /locus_tag="PK849_11555"
     CDS             complement(2132..>2740)
                     /locus_tag="PK849_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE66805.1"
                     /translation="SQENRTKMSALAEAVDSITGFVGAITGFADQTNLLALNAAIEAA
                     RAGEAGRGFAVVADEVRKLAEESNRAAREISNLMTSLSGYASESIKATEDEERTLRMV
                     VEKAEALQNTLKASMNELSSMDAELNKVSDLARTQSLSNTEMAEAVNSIARGTSDIVE
                     RLEGIRRSTMDAREAFESVTEQANTLLEGMEEMERQLGQFKV"
BASE COUNT          542 a          820 c          783 g          595 t
ORIGIN      
        1 cggagctgcg gaactcgggc tgcccgttca cttcgttcac catccagccg ccttcagccg
       61 tctcgaaaag gtcgacagcg agcacctcgc cgccgatggc gtcctgcacc ctgcccagaa
      121 gcgccttgat ctcgggcgag agcgggtact tctccggtgt cgcgccgcgc gcggtgttcg
      181 tgatccagtg ttcgctgcac cgcttgatca ccgtgatcgg ctcgccgcca agaagcgtcg
      241 cccggaggtc gaaccccttc ttctcgacgt actgctgcat gaagaggacg gagtgcgccg
      301 cgcccatgtg cgccttgtgc tctgcgacgg cctccagcgc gtcccggtcg ttgatcttcg
      361 caagaagacg cccccagctg cctgtcgcgg gcttgatcac cagcgggtag ccgaggcgct
      421 tcgctccctc gatcgctccc tccggagaga aggccaccgc gtactccggc tgcggaattc
      481 cggcggcgtc gagcttcgcg gcggtgacga tcttgtcggc gcagagggcc attacttcgg
      541 aggagttcac cgtgagcacg ccgcgcgcct ccagcagcac agcgagcgcg gagttctgcg
      601 tctggctcac gcaccggcag agcgccacgt cgccggaagc gccgggaata tcgggccaca
      661 tgccgtcgct cacgttcacc gccgagaagg gaattccccg ctcttcggcg gctttgaaca
      721 gcagtttttc ctccgtgcgg agacgggtgt agaagatgaa gaagtgtgcc atctactctc
      781 cccagtcctc ctggacctcg ggggcctcgg cgaccgtgag cggattcagg gagaccactt
      841 caagctccgt tccgcagtcg gggcaggtaa gcagctcgcc ctccatgacc tcggcgggca
      901 gcatgacatc tccttcgcag acaacgcatt gaactttagt tgacataaaa catccctcca
      961 aaacaaaatt taacaatgga gggattttaa acctgagttt gattttcgtc aagtagggaa
     1021 ataatgttcc ttttttctgt attttagact gggtgttccg gcgagaatcc tacgctctgt
     1081 gcggcattgg aggagctgca ctgaaagagg tctttacttt gtgaattgaa agcgcctttt
     1141 ggtgttataa tgaaaatatt ctttttagag ggcgtccaaa accatggaac ctttgtgctc
     1201 ggcaatcgga agacgctgaa aactgagcgc aataccctga aaaagagagg tgttttcgta
     1261 tgcgaaggat gtcgctgtta tttctttgcg ttgccgcctg catgctgtct ctcgggtcgg
     1321 ttgctcgtgc cgtcgaaatg aggatcgcgt atgaagacaa cgaacagcct ccttatatct
     1381 atggaaactc ggcggcgata ccggaaaagc cggggctttc cgtggagatg atcctcatgc
     1441 ttccggagaa gattccaggg attgaaatca agcttgtccg catgccgtgg cttcgttgta
     1501 tcgtggagct cgagaacaac gaggtggacg gcatcttcaa cgcaagctac aaaaaagaac
     1561 ggctgagaat cggacgatac ccgaccgtcg acggcacgcc cgacgggcct gtcgacgtcg
     1621 attccaggat cgccggcatg acctattcgt tctatgtact gcgcaagaac gtcggttcct
     1681 ttcaatggga cggagtccat ttcgacacga tcaggaacgc ggggactatc ggcaccccgc
     1741 tcggctactc cattgccggt gatctccgtg aacagggcgt cgccgtcgac gagtccggag
     1801 ggcccgagac gaacctggcc aagctcgccg gaggccggtt tgtggctgcg gcgctccttg
     1861 aagcgactgt cgaaggtttg attcgctcga acgctctcta tagcgaaatt gtcaaagtca
     1921 atcccccgct ggagacaaaa ccgtattacc tcatgctctc aagggccttc gtagaagaac
     1981 acccggaact ctcgcggaga atttggggcg ctctccggga gatcagggat acaaagacgg
     2041 cggaaattct tacgaggtac tgaaaaaaag gggagcgggg ccgaaaaaca gccccgctcc
     2101 cctttctctt caatagaaaa gccggcttga cttacacctt gaactggccc agctgacgct
     2161 ccatctcttc cataccttcc agaagggtgt tcgcctgttc cgtaacggat tcgaacgctt
     2221 cgcgggcatc catggtggag cggcgtatcc cctcaaggcg ttccacgatg tcggaggttc
     2281 ccctcgcaat gctgttgacg gcctcggcca tttcggtatt tgaaagcgat tgcgtgcgtg
     2341 ccaggtccga gaccttgttc agctccgcgt ccatcgaact cagctcgttc atactggctt
     2401 tcagcgtgtt ttgcaacgct tccgcctttt ccaccaccat ccggagcgtt ctctcctcat
     2461 cctcggttgc cttgatggac tcgctcgcgt accccgagag cgacgtcatc aggttggaga
     2521 tctctctcgc ggcgcgattg gactcctcgg cgagcttccg gacctcgtcg gcgaccaccg
     2581 cgaaaccgcg tcccgcctct cccgcgcgcg cggcctcgat ggccgcgttg agagcaagca
     2641 gattggtctg gtcggcgaaa ccggtaatgg cgccgacgaa ccccgtgatc gagtccaccg
     2701 cttctgcaag cgcggacatc ttcgtcctgt tctcctgaga
//
LOCUS       DAOPKU010000414         2737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_42323_length_2737_cov_5.141685, whole genome shotgun sequence.
ACCESSION   DAOPKU010000414 DAOPKU010000000
VERSION     DAOPKU010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42323_length_2737_cov_5.141685"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PK849_11560"
     CDS             <1..1228
                     /locus_tag="PK849_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPE66806.1"
                     /translation="PRCAIAACLMLSLPLLIGLAQYLPASFSAVAALSGMIFSSVGAA
                     LLIGLWALFLSSLIPDDVALVFGLAPLVAAFVMRFAALSHILLLILGAPLFSALLLLL
                     PSSDAHDESPDAIQASSFPFWRLALFLFCLYAANGFLLSLMPELFPSPFPRTDQSLDW
                     IPTISALGVAVVFRFYPRAELRTFYKGAFPFIAASLFLLLFSPLKTLSRFFMETGLAF
                     LDLYAWLLLIYFASRAGEKRNAVIHGGIFLIVLAGATAHLHLLLRNGAGGAIHGASEL
                     LIVVIPGVCLLLIMLALWDGREIPLQWERQDTRSFPERDSGEETNFDHEKMPHPHEES
                     ASEKEEEMLLRMKLMEFNLTRQETEIALLLLQGLKDRNICSLLYISQNTLKYHLRNIY
                     RKTGSANRLELKNNVD"
     gene            1418..>2737
                     /locus_tag="PK849_11565"
     CDS             1418..>2737
                     /locus_tag="PK849_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66807.1"
                     /translation="MYCRFKCVPAILFLFLLLLVLPPSGAESATPAPKNEVTRAEFFL
                     KKFEDRVNRFRGQSFRLGFEDNEALKRIRDLKEKYPEDPAVEDLYQRARMALMKSKGD
                     FMEITPEMLSFRENEQRLKKIFGEIADKEWKEFTGEILASPAVLPKAFPAPDRSQVSI
                     EDIKGHYVILDEFEYPANQFIDLGREFVSVGTGARGYYFVEISNRNWLGPYEAVKRYR
                     RLINRDFPEEGKWTVLGKIVGIEMVIPQAEKVKTVAPQWGWVVSPVALYIPGVTFAFF
                     DASIEEGGYFAGEERMEEIKDPLYTVRYVPPDATPERLVEIFAASVKEKNFDLFLDCI
                     DPNRKKTRTALSLIRYHWDLHLDRFARFYVHVTTDEAKIEVLKGFQSSAGSVEDFFLD
                     EEQQEKIKEISGELVEQATVVSKAWDERGRQYGSPKPHYLIRRDKGRW"
BASE COUNT          580 a          728 c          741 g          688 t
ORIGIN      
        1 tccccggtgt gcgattgctg catgccttat gctctcgctt cctctcctga tcggtcttgc
       61 tcaatatctt cctgcttcgt tttccgccgt tgcagcgctt tccggaatga ttttctcatc
      121 cgtcggagca gcccttctta tcggattgtg ggctcttttc ctcagctcac tcattcctga
      181 cgatgttgca ctcgtcttcg gtttggcgcc gcttgtcgct gcattcgtaa tgcgttttgc
      241 agcgctttct catatcttgc tgctgattct aggagcgccg ctcttctcgg cattgctcct
      301 gctacttccg agcagcgatg cccacgatga atctcctgac gccatacagg cctcttcttt
      361 ccctttctgg cggcttgcgc tcttcctttt ctgtctttat gccgccaacg gtttccttct
      421 cagtttgatg ccggagctct ttccctcgcc ttttccccgg acagaccagt ctttagactg
      481 gataccgacg atatctgcct tgggggttgc cgtggttttt cgcttttacc cgagagccga
      541 actgcgtacg ttctacaaag gcgcgttccc gttcatcgca gcgtcgctct tccttttgct
      601 cttctctccc ttgaagacgc tgtcccgctt ctttatggag acgggactcg cgtttctcga
      661 cctgtacgct tggctgcttc ttatctattt tgcctccagg gcaggggaga aacggaacgc
      721 ggttatccac ggaggcattt ttcttatcgt actggccggc gctacggcgc atcttcattt
      781 gctgctccgt aacggcgccg gtggtgcaat ccatggagcg tcggagctcc tgatcgttgt
      841 catccccggt gtttgccttc ttctcatcat gcttgcgctc tgggatggaa gggaaatccc
      901 tcttcagtgg gagcgtcagg atacacgctc ctttccggaa cgggatagtg gagaggaaac
      961 aaatttcgat catgaaaaaa tgcctcatcc tcatgaagaa agcgcatcgg agaaggagga
     1021 ggagatgctt cttcgcatga agctgatgga attcaatctt acacggcagg aaaccgaaat
     1081 cgcccttctt cttcttcagg ggctcaaaga caggaatatc tgttcattac tttacatctc
     1141 gcagaatacc ctcaagtacc acctccgcaa tatctatcgc aagacagggt ccgctaatcg
     1201 tctcgaactg aaaaataacg ttgactaacg aaaaagggaa tcatcaaggg tgatgccggt
     1261 caccccatgc gatagaaaaa aaggtgggga atatcacccc taaaaggggt gggaaatccc
     1321 acccctttta gttcgcggcg ggggggtgac tttctctctt tgatgctaca atccacttgt
     1381 atagacgaac cgtggaaacg agaggagatg atgcacaatg tattgtcgat tcaagtgtgt
     1441 gccggcgatt cttttccttt tcctcctcct acttgtattg ccgccctccg gagcggagtc
     1501 cgcgacgccc gctccaaaaa acgaggtgac tcgcgccgag ttcttcctta aaaaattcga
     1561 agacagggtg aaccgtttca gaggacagtc gttccgcctc ggattcgagg acaacgaagc
     1621 gctgaaacgt attcgggatc ttaaggagaa gtacccggag gatcctgctg tcgaagatct
     1681 ctatcagagg gcgcgcatgg cgcttatgaa gagcaagggc gattttatgg agatcacccc
     1741 ggagatgctc tccttcaggg agaacgaaca gaggctcaaa aagatcttcg gagagatcgc
     1801 ggacaaggag tggaaggagt tcaccgggga gattctcgcg agccccgccg tgcttccaaa
     1861 ggcctttccg gcgcccgaca ggtctcaggt ctccatagag gatatcaagg ggcactacgt
     1921 tattctcgat gagttcgagt acccggcaaa ccagttcatc gacctcggaa gggagtttgt
     1981 ctccgttgga actggagcca ggggatacta tttcgtggaa atatcaaaca ggaactggct
     2041 cggtccctat gaagcggtca aacgctatcg caggctgatc aacagagact ttcccgagga
     2101 ggggaagtgg actgtgcttg ggaagatcgt cggcattgaa atggttatcc cgcaggcgga
     2161 gaaggtgaag accgtcgccc cgcagtgggg gtgggttgtg agtcctgtgg ctctctacat
     2221 accgggcgtt acattcgcct tctttgacgc ttccatcgaa gagggagggt attttgccgg
     2281 agaggagcgt atggaggaga tcaaggaccc gctgtacacc gtccgatatg ttcctcccga
     2341 tgcgacgccc gagcgcttgg tcgagatatt cgcggcctcg gtcaaggaga aaaacttcga
     2401 tctcttcctt gactgcattg atccaaaccg caaaaagacg cgcactgcgc tctctcttat
     2461 tcgctaccac tgggaccttc atcttgatcg gtttgccaga ttctatgtcc atgtcaccac
     2521 cgacgaggcg aagatcgagg tgttgaaagg atttcaaagc agcgccggaa gcgtggagga
     2581 tttcttcctt gacgaggagc agcaggagaa gatcaaggag atctccggcg agctggtgga
     2641 acaggctaca gtcgtctcga aggcgtggga cgagcggggg cggcagtacg gctctccgaa
     2701 gccgcactac cttatccgca gggacaaggg gcgatgg
//
LOCUS       DAOPKU010000415         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_42472_length_2730_cov_4.087477, whole genome shotgun sequence.
ACCESSION   DAOPKU010000415 DAOPKU010000000
VERSION     DAOPKU010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42472_length_2730_cov_4.087477"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PK849_11570"
     CDS             <1..573
                     /locus_tag="PK849_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE66808.1"
                     /translation="KKEGLPVTCDVTPHHLSLDENCILVSRFSSTYKVNPPLRTRKDV
                     KALWEAIADGTADAIVTDHAPYHMDEKDVPFQEAPSGIASIECSVAVVLDTWLKLGEP
                     VALEHLLSLFTSGPAALLPRPWRTLGAIKEGATADITVLDLDAVKRVDVNEWKSKARL
                     CPWDGEMLQGWPVMTLVEGNVRMNRLEDGE"
     gene            570..1373
                     /locus_tag="PK849_11575"
     CDS             570..1373
                     /locus_tag="PK849_11575"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPE66809.1"
                     /translation="MSCGGSAPRDYEAKVAGSRALGDGSGLIAVECEAIASAAEPGQF
                     VMVRPGSCGDPFLGRPLAVAAASGGIFKMLYRVVGRGTELLASKRQGDSLVVRGPIGR
                     GFFSSRGDTPLPKKVLLAGGSVGAAPLLFAARRLGMAKVAGVSMGVAGRGWEGFAEWL
                     KESFPGVALFSDDGSVGTKGTALDGLPSELPADTEVWACGPQGMLRALAAKYPSDGAR
                     VRVALESRMACGMGGCLGCVIPTARGNRRVCVDGPVFTAEEVLWNEFAD"
     gene            1357..2280
                     /locus_tag="PK849_11580"
     CDS             1357..2280
                     /locus_tag="PK849_11580"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPE66810.1"
                     /translation="MSSRTEMHVSLGGLNLESPVIIASGVWPYDAELWSPERLEGVGA
                     LCTKAVSLNGRAGNKGVRVWETPCGMLNSIGLQNAGVRSFLDDYLPLVRGANRPFLVN
                     LVMESEREVRESIAALAEVPGIPAIELNISCPNVDGDGMAWGISAESAAKAVRIARSE
                     WKGALWVKLTPQSPDLAGVAKAVEAEGADAIVCGNTWLGMAMDIKKKRPAFQRVVAGL
                     SGPAVFPLALRTVWQVCGAVKIPVVGCGGVASWEDATEMLLAGAAAVEVGAALFTDFD
                     LPRKIVAGLSAYLAEQKIDSVSGLTGLGRHE"
     gene            2297..>2730
                     /locus_tag="PK849_11585"
     CDS             2297..>2730
                     /locus_tag="PK849_11585"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HPE66811.1"
                     /translation="MNNGLDRLILALDVERVEEGRELLASLKGELKYVKIGHQLYARG
                     GFPFLREIGAMGFSIFLDLKLHDIPNTVFQATEALASENLWALTLHCGGGRKMLEEAR
                     RGKEKSGSRTNLLGVTVLTSFDDASWAEVAPGCSLSDALEKRA"
BASE COUNT          442 a          827 c         1006 g          455 t
ORIGIN      
        1 aaaaaagagg gacttccggt gacatgcgac gtgacgccgc accacctctc gcttgacgag
       61 aactgcatcc tcgtcagccg cttctcctcg acgtacaagg tgaacccccc ccttcggacg
      121 cgcaaggatg tcaaggcgct ctgggaggcc atcgcggacg gcacggccga cgcgatcgtc
      181 acggatcacg ccccctacca catggacgag aaggacgtgc cgtttcagga agcgccctcg
      241 ggtatcgctt cgatagagtg ctctgtcgcg gtggtgctgg atacctggct caagctgggc
      301 gagccggtcg cgctggaaca cctgctctct ctcttcacat cgggccccgc cgcgctgctt
      361 ccccgaccgt ggcgcacgct cggagcgatc aaagagggcg cgacggcgga catcacggtg
      421 ctcgacctcg acgcggtgaa gcgggtcgat gtgaacgagt ggaagagcaa ggcgcgactc
      481 tgcccctggg acggcgagat gctgcagggg tggccggtga tgacgctggt ggagggaaac
      541 gtgcggatga atcggctgga ggacggcgaa tgagctgcgg agggagcgcg ccacgggact
      601 atgaagcgaa ggtcgccggc agccgcgccc tgggcgacgg ctccggcctg atcgcggtgg
      661 agtgcgaggc gatcgcctct gcggcggagc cggggcagtt cgtgatggtg cggcccggaa
      721 gctgcggcga cccatttctg gggcgtccgc tggccgtggc ggctgcctcc ggcgggatct
      781 tcaagatgct ctacagggtc gtcggaagag ggacggagct gctggcctcg aagcgccagg
      841 gtgattctct cgtcgttcgc ggcccgatag ggagagggtt cttctcctct cgcggcgata
      901 cgcctctgcc gaagaaagtc ctcctcgcgg gtggctcggt cggcgcggcg ccgttgctct
      961 tcgcggcgcg gcggctcgga atggccaagg tcgcgggcgt ttcgatgggc gtcgccggaa
     1021 gagggtggga ggggttcgcc gaatggctga aggagtcctt ccccggagtc gcgctcttct
     1081 ccgacgacgg aagcgtcggg acgaagggaa cggcgctcga cggcctcccc tcggagcttc
     1141 cggccgatac ggaggtatgg gcttgcggtc cgcaggggat gctcagggcg ctcgcggcga
     1201 aatacccgtc ggacggcgcg cgggtcaggg tggcgttgga gtccaggatg gcgtgcggca
     1261 tgggcggatg cctcggatgc gtcatcccga ccgcgcgcgg caacaggagg gtctgcgtgg
     1321 acggcccggt cttcactgcg gaggaggtgc tgtggaatga gttcgcggac tgagatgcac
     1381 gtttcgctcg gcggactgaa cctcgaatcg ccggtgatca tcgcgtccgg agtctggccg
     1441 tacgatgcgg aactctggtc gccggaacgg ctggaaggcg tcggcgcgct ctgcaccaag
     1501 gccgtcagcc tgaacggacg ggcggggaac aagggcgtcc gcgtctggga gacgccctgc
     1561 ggcatgctga acagcatcgg cctgcagaac gccggggtgc gttccttcct tgacgactac
     1621 ctccccctcg tccggggcgc gaaccgcccc ttcctcgtga acctcgtcat ggagtccgag
     1681 cgcgaggtgc gggagagcat cgccgcgctt gcagaggtcc ccggcatccc cgccatagag
     1741 ctgaacatct cctgccccaa cgtggacggc gacggcatgg cgtgggggat ctcggccgaa
     1801 agcgccgcga aggcggtgcg catcgcgcgg agcgagtgga agggcgcgct gtgggtgaag
     1861 ctcaccccgc agtcgccgga cctcgccgga gtggcgaagg ccgtcgaggc ggagggcgcg
     1921 gacgcgatcg tctgcgggaa tacgtggctc ggcatggcga tggacatcaa gaaaaagcgt
     1981 ccggctttcc agagggtggt cgccggtctc tcgggtcccg cggtcttccc gctcgcgctg
     2041 cgcacggtct ggcaggtctg cggcgcggtg aagattcccg tggtcgggtg cggcggcgtc
     2101 gcctcgtggg aggatgcgac ggagatgctc ctcgccgggg ctgcggcggt ggaggtcggc
     2161 gccgcgctct tcaccgactt cgacttgccc cgaaagatcg tcgccgggct ttcagcctac
     2221 cttgctgaac agaagatcga cagtgtctcc gggctgaccg gcctggggcg tcatgaataa
     2281 caggaggatc gcgaagatga acaacggact ggaccggctc attctggcgc tcgacgtcga
     2341 acgtgtggag gaggggcgcg agcttctcgc ctcgctcaag ggagagctga agtatgtaaa
     2401 gataggccat cagctctacg cccgcggagg atttcccttc cttcgcgaga tcggcgcgat
     2461 ggggttctcg atcttcctcg acctcaagct gcacgatatt cccaacactg tcttccaggc
     2521 gacggaggcg ctcgcttctg agaacctgtg ggcgctcacg ctgcactgcg gcggcggccg
     2581 gaagatgctg gaagaggccc gtcgcggcaa agagaagtcg gggtcgcgga cgaacctgct
     2641 cggcgtgacc gtcctcacga gctttgacga cgcctcgtgg gcggaggtgg ccccggggtg
     2701 ttcgctttcg gacgcgctcg aaaaacgcgc
//
LOCUS       DAOPKU010000416         2718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_42753_length_2718_cov_3.279009, whole genome shotgun sequence.
ACCESSION   DAOPKU010000416 DAOPKU010000000
VERSION     DAOPKU010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42753_length_2718_cov_3.279009"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..2041
                     /locus_tag="PK849_11590"
     CDS             227..2041
                     /locus_tag="PK849_11590"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPE66812.1"
                     /translation="MLYRFSHSLPPFAANGKRSMYRVVPALVFFLLSLALVFPALKAN
                     AAASVRKDAFIAQLFQARGFSLPANEKSPVNAALEYNLTPLPEGKLSDPLTRREAIVY
                     AVHSLGLFFESRILADYPLPYKDVSGLLPEERGALAVALNMKPPLLKKGSATFSPSHA
                     VSPAEANEIAARVKAATRELRLSVDLSPLKGMTIRIRREGVLSILPKWRAVVNGFESR
                     EDADFFRGLLTERGVEATVDSYNYDWRVRSPIFDMYGGVRKFLSAASALGREGVVFTS
                     IPNWEMTDTPRFWTMMVFDPGAFELRPVFPSEGMAALAPLSSMLRDGAVAVVNGGYFG
                     TSGKGRGSPIGALMTDGVLLNPPFAGRTCFGWNSENRAVFGQLTWNGKVHLPGGFMEL
                     SGINRTLKGDGVVLFSPHFGMTTPLVETRAAEAVLGGSRVEAVRTGGGNPIPDGKLVL
                     AVYGAASRFIESLRFGETVSLSLQLNEGDPAWSSMSHIVQGGPFLLRNGEVLSEREKL
                     SDSIIDRRHPRTVVGLTKEGHWFFFVGDGRNAVHSVGFTLDEVSRILKSAGVSYALNL
                     DGGGSSGIISSGGFWNIPSDGLERPVSYGIGAFQRRGR"
     gene            2149..2634
                     /locus_tag="PK849_11595"
     CDS             2149..2634
                     /locus_tag="PK849_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66813.1"
                     /translation="MKINVRTPVILAVALLVALFVAGSVASASVQPVNFGQDHIKMQW
                     YLVNYGEENGVHFANARKYYTNPDVKAETIALIVEKFGTDQELANGLYFTEYGYAYSK
                     DGKSFAVTYINHYDMLGKVIRATEYDEASREFVGMSKDMIPFKAYAYATGKAKPAAKK
                     K"
BASE COUNT          516 a          785 c          803 g          614 t
ORIGIN      
        1 gagcggcggt ctacggaacc gcaggtcacg agttcgaatc ttgtccggcg cgccattttg
       61 ttctccagaa ccggggtccg attatcgggt cccggttttt ttacgccttt ttccgtatgc
      121 cgcgcttcga gcacatctgg tacactcgtc atgttatatg cgcgcagttg cggcatctgg
      181 aattatcttc gccgtttcga atacagcgcc tcaaggagaa tacactatgc tctatcggtt
      241 ttctcattct cttcccccct tcgccgctaa cgggaagagg agtatgtaca gagtagtccc
      301 ggccctcgtc tttttcctcc tctctcttgc ccttgtcttc ccggccctga aagcgaatgc
      361 cgccgcttcc gtccgcaagg atgcgtttat cgcccagctc tttcaggcgc gggggttttc
      421 gctgcctgca aacgagaagt cccctgtcaa cgctgctctt gaatataacc tgacgcctct
      481 cccggaggga aagctttccg accctctcac gcgcagggag gcgatcgtgt atgccgtcca
      541 cagcctcggg ctcttcttcg agtcgcgtat cctcgccgac tatccgcttc cctacaagga
      601 cgtctccggt cttttgccgg aggagcgggg cgctctggcg gttgccctga acatgaagcc
      661 tcccttgctg aaaaaaggtt cggcgacttt ttctccgtcg cacgcagtct ctcccgccga
      721 agccaatgag atcgccgccc gtgtgaaggc ggcgacccgc gagctccgcc tctcggtgga
      781 cctctctcct ctcaagggaa tgacgatccg gatacgccgg gaaggcgtgc tctcaatcct
      841 tccgaagtgg agggcggttg tgaacgggtt cgagtcccga gaggacgcgg actttttccg
      901 cgggttgctt acggaacgcg gcgtggaggc tactgttgac agctacaact acgactggcg
      961 cgtgcgctct cccatcttcg acatgtacgg cggggtacgg aagttccttt ccgcagcatc
     1021 cgcgctcggc agagagggcg tcgtttttac gagcattccg aactgggaga tgaccgatac
     1081 gccgcgcttc tggacgatga tggtcttcga tcccggggcg tttgaactgc gccctgtctt
     1141 tccctccgag ggaatggcgg cgctcgctcc cttgagctcc atgctccgcg acggcgccgt
     1201 cgccgttgtg aacggagggt atttcgggac gtcggggaaa ggccgcggaa gccctatcgg
     1261 cgcgctgatg accgacgggg tgctgctgaa tcctccgttc gccgggcgta cctgcttcgg
     1321 atggaacagc gagaacaggg cggtcttcgg tcagcttacg tggaacggga aggtgcatct
     1381 tccgggaggg ttcatggagc tcagcggaat caaccggacg ctgaaggggg acggcgttgt
     1441 cctcttctct ccgcacttcg gcatgaccac ccctcttgtc gaaacccgcg cggccgaggc
     1501 tgttctcgga ggctcgcgcg tggaggcggt ccgtacaggg ggaggaaacc cgatacccga
     1561 cgggaagctc gttctcgcag tctacggggc ggcgtcgcgc tttatcgagt ctctgcgttt
     1621 cggcgaaacg gtcagcctct cgcttcagct gaacgagggg gatccggcgt ggagttcaat
     1681 gtcgcatatc gtacagggag gccctttcct tctccggaac ggggaagtcc tttccgagag
     1741 agaaaaattg agcgactcga ttatcgatcg ccgtcacccc aggactgtcg tcgggctgac
     1801 gaaagagggg cattggttct tcttcgtagg cgacggaaga aatgctgtgc acagcgtagg
     1861 cttcacgctc gacgaggtct cccgcatcct gaagagcgcc ggggtttcct atgcgctgaa
     1921 cctcgacgga ggcggttctt cggggattat ctccagcggg ggtttctgga atatcccttc
     1981 cgatggcttg gagcgtccgg tgagttacgg catcggagcc tttcaaagaa gggggcggta
     2041 gagaaaggaa cgaagaaaat gctccccgtg cgttttattt tcgccgtttt gtggtagact
     2101 acacggcgta aggcagatga aaaattggaa ggagttgtct cattcagcat gaaaatcaat
     2161 gtacgtacac ccgttatttt ggcagtcgct cttcttgtcg cactctttgt cgccggttcc
     2221 gttgcatccg cctcggtgca gccggtcaac ttcggtcagg accacataaa gatgcagtgg
     2281 tacctcgtga actacggcga agagaacggc gtccacttcg cgaacgccag gaaatactac
     2341 acaaatccgg acgtcaaggc cgagaccatc gcgctcatcg tcgagaagtt cggcaccgac
     2401 caggagcttg ccaacggatt gtacttcacc gagtacggct acgcctattc gaaggacggc
     2461 aagtctttcg ccgtgacgta catcaatcac tacgacatgt tgggaaaagt catccgcgcc
     2521 accgagtacg acgaggcttc acgagaattc gtcggaatgt ccaaggacat gattcccttc
     2581 aaggcgtacg cctacgccac aggaaaagcg aagcccgccg caaagaagaa gtagctgagc
     2641 gtctttacgt actgtcaagg cctggcctga ggccaggcct tgttttattt tgacccgagg
     2701 aggttgtttc catgcccg
//
LOCUS       DAOPKU010000417         2713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_42862_length_2713_cov_3.769375, whole genome shotgun sequence.
ACCESSION   DAOPKU010000417 DAOPKU010000000
VERSION     DAOPKU010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42862_length_2713_cov_3.769375"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..531
                     /locus_tag="PK849_11600"
     CDS             136..531
                     /locus_tag="PK849_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPE66814.1"
                     /translation="MRKKPDRYVFPAVFTYDGDGVAVSFPDLPGCNTCGSDQDEAILM
                     AQDALRGHLSCMEDDNDPIPAPSDFRSVKVDVDEAVVLVEAWMVPLREKTVRKNLTIP
                     AKLAYKAERAGINFSGVLTKALEKELQRL"
     gene            complement(577..987)
                     /locus_tag="PK849_11605"
     CDS             complement(577..987)
                     /locus_tag="PK849_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPE66815.1"
                     /translation="MAAKKDLRIYPALFHFEEEDKLFCVEYPDLPGCVTVGENMEIAL
                     RRAKEALEGFLYSSEQNGYPVAPPTPLEKMSVPAEHILVPVSVRMDILREEMANRSIT
                     KNVTIPAWLNKMAIEHKINFSSTLQEALRERLGV"
     gene            complement(1019..1213)
                     /locus_tag="PK849_11610"
     CDS             complement(1019..1213)
                     /locus_tag="PK849_11610"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HPE66816.1"
                     /translation="MIKNKTSKEMIEIIEADGWTPDRIRGDHHQFVHTTKPGVVTIPH
                     PEKNLGSWLIGKILKQAGLK"
     gene            1412..1711
                     /locus_tag="PK849_11615"
     CDS             1412..1711
                     /locus_tag="PK849_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPE66817.1"
                     /translation="MTQLERTVSILEANKELLKQFHVCSLSVFGSVARGEAGPDSDID
                     LLVDFEPGARIGFVAFARLQKCLEELLGTKVDLVTPDALHEGMRAEIIEEAVHAI"
     gene            1701..2069
                     /locus_tag="PK849_11620"
     CDS             1701..2069
                     /locus_tag="PK849_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPE66818.1"
                     /translation="MRSRSWKLRIRDIVDAVRTILGYLGYIEGMTFDDFVRDQRTMDA
                     VVRRLTIIGEAAARVPELVCEKYRHIPWAEMRAMRNFIVHEYFGISEQILWETIQMDL
                     PAIVGPLEALLVSGKEVDET"
     gene            2401..>2713
                     /locus_tag="PK849_11625"
     CDS             2401..>2713
                     /locus_tag="PK849_11625"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HPE66819.1"
                     /translation="MPPQSLSHSLSLPHPLSSWPGESVAFLVGIHDIGKTDAYFQHQV
                     PEFKKDLEDAGYFPTGDSHYRHERLSAHFLKNWLLENEISTRVADALSRAVLVHHGYW
                     EG"
BASE COUNT          627 a          666 c          744 g          676 t
ORIGIN      
        1 ctgttctctt gctctaaagg aggaagtggt atcgcgcttt tgaagactag tttttttgca
       61 ttgtatgcgc atggtataat aaaaaccaga aaagggggag gtgtgcaata ccgacaacga
      121 aggagttggc cgaaaatgag aaaaaaaccg gatagatacg tgttccccgc tgttttcacc
      181 tacgacgggg acggcgtggc ggtctccttc cccgaccttc ccggatgcaa cacctgcggg
      241 agcgatcagg acgaggccat ccttatggca caggacgcgc tgagagggca tctctcttgc
      301 atggaggacg ataacgaccc catccccgcc ccgtcggact tccgatcagt gaaagtggac
      361 gtcgacgaag ccgtggtgct tgtagaggcg tggatggttc cgcttcggga aaaaaccgtc
      421 cgtaagaacc tgaccatacc ggcaaagctg gcatataagg ccgaacgcgc ggggatcaac
      481 ttttccggcg ttctgaccaa agcgctggaa aaagagcttc agcgtttata acgcgcgtcg
      541 ccccggattg gactgccgat caggggcgat gtcctttcag accccgagac gctcgcgaag
      601 cgcctcctga agcgtactgg agaaattgat cttatgctct atggccatct tgttcagcca
      661 tgcgggaatg gtgacgttct tcgtaatgct tctgttggcc atctcttcgc gcaggatgtc
      721 catacgaacc gaaacgggaa caagaatatg ttcggccggt acgctcatct tctcaagagg
      781 agtaggtgga gcgacagggt agccgttctg ttcggaagag taaagaaacc cctcgagcgc
      841 ttccttcgcc ctgcggaggg ctatttccat gttctccccg actgtgacgc atcccgggag
      901 gtcggggtat tcgacgcaga aaagcttgtc ctcttcttca aagtggaaga gagccggata
      961 gatgcgcaga tcctttttcg ccgccattgc agatcactcc tttgcgggtg aagcaggctt
     1021 atttcagtcc cgcttgtttg agaattttgc cgatcagcca gcttccgagg ttcttctccg
     1081 gatgcgggat ggtgacaact ccgggtttcg ttgtgtgaac aaactgatgg tgatctcctc
     1141 tgatgcggtc cggagtccat ccgtcagctt cgatgatctc aatcatctct ttcgaggttt
     1201 tgtttttgat catttccgcc ccctctcatc cgaggtgatt gtaacactta ttattatagg
     1261 tgtcaaaagg gggttgcatg ttagccgata gagaagtgca atccacatgg aggcgtggtt
     1321 cctcccgtgc cgcctcgaag acgatcattt tctttgtgtg tatggcggcg gagtaacgat
     1381 gatatacttt ccgataagga ggcgaattgc catgacgcag cttgaacgga cggtgagcat
     1441 ccttgaagcc aacaaggaac tgttgaagca gttccatgtt tgctcattat cggtgtttgg
     1501 ttcggtcgca cgcggagagg ccgggccgga cagcgacatc gacctgctgg tggacttcga
     1561 acccggcgcc cggattgggt tcgtcgcctt cgcccgcctt cagaagtgcc ttgaggaact
     1621 gctcggaacg aaggtcgacc tcgttacgcc cgatgcgctg catgaaggaa tgcgagcgga
     1681 gatcatcgag gaggccgtgc atgcgatcta gaagttggaa gcttcgtata cgggatattg
     1741 tcgatgctgt tcgtacgatc cttggttatc ttggttatat agaggggatg accttcgacg
     1801 acttcgtccg ggaccagcgc accatggacg cagtagtgcg ccgccttacg atcatcggcg
     1861 aggcggcggc ccgtgtgccg gaattagtct gtgaaaagta tcggcatatc ccttgggccg
     1921 aaatgcgcgc gatgaggaat ttcattgtgc acgagtactt tggtatcagc gaacagattc
     1981 tctgggaaac gatacaaatg gatcttcctg ctattgtagg gcctttagaa gctctgcttg
     2041 tatcgggaaa agaagtggac gaaacataga aggtcaaatc gctattgctc tgcccaaaaa
     2101 ttaaacatca actctacgct gcgcacgcta cgcgcatcgc cccggatcgg actgctgatt
     2161 cggggcgatg cgcgttcagg cgcttagtgg aaaagtgatt atcaggcttt atagaaaagt
     2221 cgacggatta gtagtataat ccggattaat tgaaggattc ctagcttgag tgatagttag
     2281 gttaatttgg gaaaaagtgg tgatacaagc aaatgactga ttccatatta ctgccgcaaa
     2341 actgcaaagc acttcgggca aaaagcaatc ctaagcatcc tttgtggaaa catcttctcg
     2401 atgccgccgc agtcgctctc tcactctctc tccctccctc atcctctctc aagttggcct
     2461 ggtgaaagcg ttgcttttct ggtcggaatc catgatattg ggaagacgga tgcttatttt
     2521 caacatcagg ttccggaatt caaaaaagat cttgaagatg caggttattt ccccactggc
     2581 gattctcatt accgccacga acgactctcc gcacacttcc ttaaaaattg gcttctcgaa
     2641 aacgaaatca gtacgcgagt tgctgacgct ctttctcgcg ccgtactggt tcatcatggt
     2701 tattgggaag ggg
//
LOCUS       DAOPKU010000418         2705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_43005_length_2705_cov_4.883019, whole genome shotgun sequence.
ACCESSION   DAOPKU010000418 DAOPKU010000000
VERSION     DAOPKU010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43005_length_2705_cov_4.883019"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PK849_11630"
     CDS             complement(<1..325)
                     /locus_tag="PK849_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66820.1"
                     /translation="MAVVEMTGISMVGPREEIERVAAGLLSLGNFEPVPLDFAMEKHP
                     GALSGSGGSRIATFRENPYDELLEVLCEIQTLVSEEALQGETSGGLFPGKSGTSALSR
                     TSPGEL"
     gene            complement(316..1446)
                     /locus_tag="PK849_11635"
     CDS             complement(316..1446)
                     /locus_tag="PK849_11635"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPE66821.1"
                     /translation="MSFLSAGERAAVTGKARVFRGRLLAPDDYLHLLKCGTVGEVIMY
                     LGRTPSYSSYFHGEYSQDIHRGEIEGILSIVPMLEELPFCRYLGPERASLLHAWGERF
                     DLEVVKRVLRIIATGMGDREALRRRVSSVPITLADAKKLLAAQTFRDVIEAIKGYPLE
                     NVLTEPLRQASETYSGGSVNLFRAKMAMDAFFMTRILSAGEKLPGSEGKGIRSIFGTR
                     ADLINLYWIYRGRRFFAMTPEEALAITLPVRYTLNFDTLRSFAFAPDIPAMLRLIQEC
                     PYGAAFTTALGESTLEVSEIILEHNLYRILWRAATKIFASGTSGVHVVLAYLILRELE
                     VKDLFTIIEDIRYDYDKKRAREFLIHPGANAFTAGDRGMSWQ"
     gene            complement(1469..1804)
                     /locus_tag="PK849_11640"
     CDS             complement(1469..1804)
                     /locus_tag="PK849_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66822.1"
                     /translation="MNLSAVLGELLASEDEAVNGVETAKRKASKILREAREHFAADQE
                     ARLAGVRAQSKTIVESALHSAELEAAQIAEMGANGRRKMEEHFTESAPEIVRSLVMGT
                     AARYGRLGG"
     gene            complement(1801..2433)
                     /locus_tag="PK849_11645"
     CDS             complement(1801..2433)
                     /locus_tag="PK849_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPE66823.1"
                     /translation="MTQKIAPTRGNMVKFASSLKLARKGRDLLEQKRTILLMELTGKI
                     REARDLQRDLELVFSDAYFSLQMAGLSVGIDNVEELAYSVPEVQDFTIRLHSVMGVEI
                     PKVDPMEKPPVPCYSFLGTSGALDAAYSRAQDVLALIARLAEVETSVYRLAVQIRKTF
                     RRVNALEKVVIPSNKRTLAWIANVLEENDREDFTRMKMARKNTSEGGGAR"
BASE COUNT          521 a          936 c          750 g          498 t
ORIGIN      
        1 agagctctcc gggggacgtc cgcgaaagcg cggatgtccc gctctttccg ggaaagagcc
       61 cgccggaggt ttctccttgc agcgcttcct ccgacacgag cgtctgtatt tcgcagagca
      121 cctccagaag ctcgtcgtac gggttctccc tgaacgtcgc gatccgcgac ccgccggagc
      181 ccgacagggc cccggggtgc ttctccatag cgaaatccag cgggacgggc tcgaagttcc
      241 cgagggagag cagacccgcc gcaacgcgct cgatctcctc ccgcggaccg accatcgaaa
      301 ttcccgtcat ctccactact gccatgacat cccccgatct ccggctgtga aggcgttcgc
      361 cccggggtgg atcaggaatt cgcgcgccct cttcttgtca tagtcgtacc ggatgtcctc
      421 aataatagtg aacagatcct tcacctccag ttcccgcagt atcagatacg ccagcaccac
      481 atgcaccccg gaagttcccg aggcgaaaat cttcgtcgcc gccctccaca ggatgcggta
      541 gaggttgtgc tccagaataa tctcgctcac ctcgagcgtc gactcgccga gcgccgtcgt
      601 gaacgccgct ccgtacggac actcctgtat cagacgcagc atcgccggaa tgtccggggc
      661 gaaggcgaac gaacgcaatg tatcgaaatt cagcgtgtac cgcaccggca gcgtgatcgc
      721 aagggcttcc tccggcgtca tcgcaaaaaa acgtcgtccc cggtagatcc agtagaggtt
      781 gatcaggtcg gcacgggtgc cgaagatgct ccggatgccc ttcccctcgc ttccgggaag
      841 tttttctccc gccgacagga tccgcgtcat gaagaaggcg tccatcgcca tcttcgcccg
      901 gaacaggttg accgatcctc ctgagtacgt ctcggacgcc tgccgcagag gctccgtcag
      961 cacgttctcc aaagggtacc ccttgatcgc ctcgatcacg tcgcggaagg tctgcgcggc
     1021 gagcagcttt ttcgcgtccg ccagcgtaat cggtacggag gacacccggc ggcgcagcgc
     1081 ctccctgtcg cccatacccg tcgcgatgat gcgcagcacg cgcttcacca cctccaggtc
     1141 gaagcgctct ccccacgcat gcaaaaggga ggcccgctca ggcccgaggt accggcagaa
     1201 aggcagctct tccagcattg gaacgatcga gaggattcct tcgatttccc cgcggtgaat
     1261 gtcttgggag tactctccat ggaagtacga ggagtaggaa ggggtgcgcc ccagatacat
     1321 gatgacttcc ccaaccgttc cgcacttcag gagatgcaga tagtcgtcgg gggcgagcag
     1381 ccgtccccgg aagacgcgcg cctttcctgt taccgccgcc cgttcgcccg ccgaaagaaa
     1441 ggacatgcgc acccctccgt acttctggtc atcctcccag tctcccgtag cgcgccgcag
     1501 tgcccatgac aaggctccgc acgatctccg gggcggactc ggtgaaatgt tcctccatct
     1561 tccgtcgtcc gttggctccc atctcggcga tctgcgccgc ctcaagctcc gccgagtgca
     1621 gggcggactc cacgatcgtc ttcgactgcg cccggactcc ggccagcctc gcctcctggt
     1681 ccgcggcgaa atgctcccgc gcctcgcgga gaatcttgga cgccttccgc ttcgccgtct
     1741 ccactccgtt gacggcctcg tcctccgagg cgagaagctc gcccagcaca gcgctcagat
     1801 tcatcgcgca cctccccctt ccgaagtatt ctttctcgcc atcttcatgc gcgtgaagtc
     1861 ctcccggtcg ttctcctcaa gcacgttggc gatccacgcc aaggtcctct tgttcgacgg
     1921 aatgacgacc ttctccagcg cgttcaccct ccggaacgtc ttgcggatct gcaccgccag
     1981 ccggtagacc gacgtctcca cctccgccag ccgcgcgatg agcgcaagca cgtcctgcgc
     2041 ccggctgtag gcggcgtcga gcgctcccga cgtcccaaga aaggagtagc agggaacggg
     2101 cggcttctcc atcgggtcca ccttcgggat ctccaccccc atcacggagt gcaggcggat
     2161 ggtgaagtcc tgcacctcgg ggacggagta ggcaagctcc tccacgttgt cgatccccac
     2221 ggagagcccg gccatctgca ggctgaaata ggcgtcggaa aagacaagtt cgaggtcgcg
     2281 ctgcagatcc cgcgcctcgc gtatcttccc cgtcagctcc atcagcagga tcgtccgctt
     2341 ctgctccagg aggtcgcgcc ctttccgggc cagcttcagc gacgacgcga acttcaccat
     2401 gttgccccgc gtgggggcga tcttctgggt cacacaccgc acctcccgtt cacatgatga
     2461 gcactttaaa actccgggga agaaaagtca acaacaaaga aatagactgc gacagaacag
     2521 aaaaagaagg aaaatatatc tctcgtgtcc gcaacagccc gaaaaagacg agcgcgccgc
     2581 atacgttcac tcggcgcgct cgtctttcaa tgcatacggt cttccgctgc tctttgcttg
     2641 ttacagccgc agcaacagca aagccagggg ggccagcagg acgagcgacg ccgcgccgaa
     2701 gagcg
//
LOCUS       DAOPKU010000419         2692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_43301_length_2692_cov_4.782328, whole genome shotgun sequence.
ACCESSION   DAOPKU010000419 DAOPKU010000000
VERSION     DAOPKU010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43301_length_2692_cov_4.782328"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1582
                     /gene="istA"
                     /locus_tag="PK849_11650"
     CDS             233..1582
                     /gene="istA"
                     /locus_tag="PK849_11650"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HPE66824.1"
                     /translation="MTTQYREILRLASLGLSGRSIAGSVSCSRNTVSEVLARAAKKGL
                     VWPLPLEVGEGDLRKLLFPEKEKDASSRTPDCEAIHRELAKSGVTMTLLWDEYCRKCR
                     EGGEIPYQYAQFCKIYRKYAAVSKATMHIERKPGERMEVDWAGKGMKIRDSVTGEERG
                     MHLFVAVLPASQYAYVEAFPDMGMESWIRGHTNAFTHFGGVPRMVVPDNLKTGVEKAD
                     WYSPVVNRTYHEMAEHYGTAIVPARVRHPKDKASVEGTVGHITTWIAAALRNRTFFTV
                     GELNREIRKKLEELNERPFQKRPGSRLSVFLEEERDFLFPLPASSYEIAVWKTATVSF
                     NYHVEVERNFYSVCHEYIKQKVDVRVTSRMVEIFYNGVRICSHPRVNGAPGQYRTVPE
                     HMPESHRQSAEWSGERFVSWASSIGPNTEAVVRSVLAGCKVEQHKQGTGEVRAVSFA"
     gene            1619..1846
                     /locus_tag="PK849_11655"
     CDS             1619..1846
                     /locus_tag="PK849_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66825.1"
                     /translation="MGILKMADKYSVERLENACARALSYTPRPTSRNISAILKSGQDK
                     ALPAAEPTRKPDERHSFIRGAEYYGGGRDAD"
     gene            1836..2600
                     /gene="istB"
                     /locus_tag="PK849_11660"
     CDS             1836..2600
                     /gene="istB"
                     /locus_tag="PK849_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HPE66826.1"
                     /translation="MLTEPTVDKLHEMKLRGMADAFRQQLRNPSMEALSFEERFGMLV
                     EAEYSKRRNSLMDRLVTNAGLKIGDACMEGIEYHPDRKLNRELLSTLATCSYIRERRD
                     VLLVGATGAGKTYIACALGNAACRNYITVKYMRLPELLTDLSIGRGDGTIKKLLNHYK
                     RKVSLLILDEWMLTPLSGLAAFDLLEIVEARHQNASTIFISQCGPAGWHEHIDDGRVA
                     DAILDRIVHNAYEIVIDGEDSMRWRKSFKRKTGTKG"
BASE COUNT          636 a          733 c          858 g          465 t
ORIGIN      
        1 acgtcgacat cttctcccag gtattcgaac ggaacggagt agaatttgcg ttgactgaat
       61 atcacacagc gggagagccg cttgtctcca aaaagagagc cacggttctc ccggaataga
      121 gccacgtata tccagaaata gagccgggat ttctcctgcc cgagagccgt tttccctaca
      181 attcgaatgt gcgcgaatgc gtgcgaattc gaatcaggga ggttttagtg gtatgacgac
      241 gcagtacagg gagattctgc ggctggcctc gctcgggctg agcgggagga gcattgcggg
      301 gagcgtttcc tgctcccgca acaccgtttc ggaagtgctg gctcgggcgg cgaagaaagg
      361 actggtatgg cctctgccgc tggaagtcgg cgagggggac ttgagaaagc tgctctttcc
      421 cgagaaggag aaagatgcgt cgtcccggac gccggactgc gaggccattc accgggaact
      481 ggcgaagtcg ggagtgacga tgacgcttct gtgggacgag tactgccgga agtgtcggga
      541 agggggcgag attccgtatc agtatgcaca gttctgcaag atctaccgca agtacgccgc
      601 agtctccaag gcgaccatgc atatcgagag gaagccggga gaacgcatgg aggtggactg
      661 ggcggggaaa gggatgaaga ttcgcgactc cgtcacaggc gaagaaagag ggatgcatct
      721 cttcgtcgcg gtgcttccgg cgagccagta cgcttacgtg gaagccttcc cggacatggg
      781 tatggagagc tggattcgcg ggcatacgaa cgcttttacg cacttcggcg gagttccccg
      841 catggtggtg ccggacaatc tcaagaccgg cgtggagaag gcggactggt attctccggt
      901 cgtcaacaga acgtaccacg aaatggccga gcactacgga acggcgatcg ttccggccag
      961 agtacggcat cccaaggaca aggcgagcgt cgagggaacg gtcggccaca tcaccacctg
     1021 gatcgcggcg gcgctgagga acagaacctt cttcacggtg ggggaactca accgcgagat
     1081 tcggaagaag cttgaggaat tgaacgagcg ccccttccag aagcgccccg gaagcagact
     1141 gagcgtcttt ctggaggaag agcgggattt ccttttcccc ctgccggcga gttcctacga
     1201 aatcgccgtg tggaagacgg cgaccgtctc cttcaactac cacgtcgagg tggagaggaa
     1261 tttctacagc gtgtgccacg agtacatcaa gcaaaaggtc gacgtccgcg tcacgagcag
     1321 gatggtggag atcttctaca acggggttcg catatgctcc caccccaggg tgaacggcgc
     1381 tccgggacag taccggacgg tgccggagca catgccggaa agccaccgtc aaagcgcgga
     1441 gtggagcgga gaacgcttcg tttcgtgggc ttcgagcatc ggaccgaaca cggaggcggt
     1501 cgtccgaagc gtgctcgccg gatgcaaggt ggaacagcat aagcagggga ccggcgaggt
     1561 acgagccgtg tccttcgctt agtgcgcatg catccggcgc tagggttttc ggacatgcat
     1621 gggcatcctg aagatggcgg acaagtactc cgtcgagcgt ctggaaaacg cctgcgcaag
     1681 agcgctttcc tacaccccgc gcccgacgag cagaaacatc tcggcgattc tcaaatccgg
     1741 gcaggacaag gcgcttccag cggccgaacc gacgagaaaa ccggacgaac ggcacagctt
     1801 catccgggga gcggagtact acggaggcgg acgcgatgct gactgaaccc accgtggaca
     1861 agctgcacga aatgaagctc cgcggcatgg ccgacgcctt ccgccaacag ctccggaatc
     1921 cgtcgatgga agcgctctcc ttcgaggagc gcttcggaat gctggtggag gccgaatact
     1981 ccaagaggcg gaactctctc atggaccgcc tcgtgacgaa cgccgggctg aaaataggcg
     2041 acgcgtgcat ggaaggcatc gaataccacc cggacagaaa gctgaacaga gagcttctct
     2101 ccacgcttgc gacctgctcg tatataagag agcgccggga tgttcttctg gtcggcgcta
     2161 cgggcgcagg aaagacctat attgcttgtg ctctcggcaa cgccgcatgc cggaattata
     2221 tcaccgtcaa gtacatgcgg ctccccgaac tgctcacgga cctgtcgata ggcagagggg
     2281 acggcaccat taaaaagctg ctgaatcact acaagagaaa ggtctcgctc ctcatactgg
     2341 acgaatggat gctgacgccg ctgagcggct tggccgcgtt cgatttgctg gaaatagtcg
     2401 aagccagaca ccagaacgcg tcgacgatct tcatctcgca gtgcggaccg gccggatggc
     2461 acgagcatat cgacgatggg agagtggccg acgccattct ggacaggatc gtccacaacg
     2521 cctacgagat cgtgatcgac ggcgaggact ccatgaggtg gagaaagagc ttcaaacgga
     2581 aaaccggaac caaggggtaa ctacgtcacg aacggcgaag ccccgcaatg gcggcttcgc
     2641 cgccacaaga ccggaaaggg tggctctaag aacagatatc cgtggctcta aa
//
LOCUS       DAOPKU010000420         2673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_43738_length_2673_cov_5.550038, whole genome shotgun sequence.
ACCESSION   DAOPKU010000420 DAOPKU010000000
VERSION     DAOPKU010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43738_length_2673_cov_5.550038"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..588)
                     /gene="rplT"
                     /locus_tag="PK849_11665"
     CDS             complement(229..588)
                     /gene="rplT"
                     /locus_tag="PK849_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPE66827.1"
                     /translation="MPRVKGGSASDKKRKKLFSITKGYWGRKKNVYRRAREAYLHSLS
                     RAYVDRKRKKRDFRRLWITRINAATRSCGMSYSAFMNGLKKSGILINRKMLSELAIND
                     MAAFTQLTVQAKNALGK"
     gene            complement(623..820)
                     /gene="rpmI"
                     /locus_tag="PK849_11670"
     CDS             complement(623..820)
                     /gene="rpmI"
                     /locus_tag="PK849_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPE66828.1"
                     /translation="MPKLKSHSGAKKRFSFTGTGKLSYRRVGRGHHLTVKGPARLRKL
                     RKTGYVDATRIESLKKLLPYN"
     gene            complement(923..1540)
                     /gene="infC"
                     /locus_tag="PK849_11675"
     CDS             complement(923..1540)
                     /gene="infC"
                     /locus_tag="PK849_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPE66829.1"
                     /translation="MNREITSREVLIIDENGVKLGTLPTAEGIRMAEERMLDLVEVAP
                     QATPPVCRILDYGKYRYQLQKKEKDARKKQKVQTLKEMKMRPKIDEHDYDFKVRAIKS
                     FLEDGHRVKVSVFFRGREMSFLDKGEAVLARVMRDCDGLGKSEGNPRMEGRYMRIMLT
                     PVPQAQIKPTPKPAAKNDVPKAEKKPDETKVNPAPEGAESRQVSE"
     gene            complement(1755..>2673)
                     /locus_tag="PK849_11680"
     CDS             complement(1755..>2673)
                     /locus_tag="PK849_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE66830.1"
                     /translation="IPSLKATMLDKGHMIIPLAVIIYFLVAGYTPLYSAFLGLFAIIA
                     VSSLKKSTRMGLKDVVRALDEGTRSAAPVGISCAIVGFIVGAVGMTGLGQVIAMNIIM
                     FAGGKLWAALILCMIAAIILGMGLPATPCYIITATIAAPALQQMGVPPLAAHFFAFYY
                     GTMSAVVPPVALTSYTAAGLAGARPFKVAMSALGLAVSGLLIPYLFVYNPDLLFVDFV
                     LTKYVLDLSIAIIGIFSLSCGIIGMLRREMEFWERALFVAAGILMVNPLRVLRISSLG
                     FFALILAFHLFSSRGANAQRSGGETKEEK"
BASE COUNT          572 a          752 c          699 g          650 t
ORIGIN      
        1 ggagagcgca gcgtcttcgg tagaaaagaa ggacggaaac attatacttc gttcgaaaat
       61 ttcactccag gtggattata tcaccacatc cgccgaggct ccacaggcaa agaaaaaggg
      121 ttcccgccgc tgcgtggcgg cagaaaccct cgaaatggcc ttttaggagg ctgcgcggtt
      181 gcgtgtagtg cgccgcacaa cctgtctcgc gcttttaaag gcgctggtct acttgcccag
      241 tgcgttcttt gcttgaaccg tcaactgggt gaacgcagcc atgtcgttga tcgccagctc
      301 ggaaagcatc ttcctgttga tgaggattcc cgattttttc aggccgttca tgaaggcgct
      361 gtaggacatt ccgcaggatc tggttgcagc gttgattctt gtgatccaca ggcggcggaa
      421 atctctcttc ttgcgttttc tgtcaacgta cgccctggaa agggagtgaa ggtacgcctc
      481 tctcgctctc ctatacacat tctttttccg tccccagtat cctttggtta tcgaaaaaag
      541 tttttttctc tttttatcgc ttgcgctacc acctttgacg cggggcatcg cacgtcacct
      601 ctttcataga ataaacttgt tctcagttgt acgggagaag cttcttcaga ctctcgatac
      661 gggttgcgtc gacataccct gttttgcgca gcttgcgaag gcgagcggga cccttcaccg
      721 taagatgatg gcctcttccc actctccggt aggagagctt ccccgttcct gtgaacgaaa
      781 accttttttt ggctcccgaa tgggatttaa gctttggcat ttgagcattc ctccttcctc
      841 ggatattcat caagataaaa gcccgccgca gttcgcgaca ggcggattga acgtcatcgc
      901 attccaagcg tcctgttcgg acttattctg aaacctgccg gctttccgct ccctcggggg
      961 cggggttcac ttttgtttcg tccggcttct tttcagcctt gggaacatcg tttttcgcag
     1021 cgggcttggg cgtcggcttt atctgggcct gaggtacagg agtcagcatg atccgcatat
     1081 accgcccctc catgcgggga tttccctcgc tcttgccgag tccgtcgcaa tctcgcatta
     1141 cccgggcgag aactgcctct cccttgtcaa ggaaggacat ctctcttccc ctgaagaaga
     1201 cggagacctt gactctgtgt ccgtcctcaa ggaaactttt gatggccctg accttgaagt
     1261 cgtagtcgtg ctcgtcgatc ttgggccgca tcttcatttc cttcagggtt tgcactttct
     1321 gcttcttccg ggcgtccttc tcttttttct gtaactggta ccggtattta ccgtaatcga
     1381 gaattctgca tacgggaggc gttgcctgcg gcgccacctc aacaaggtcg agcattctct
     1441 cctcggccat ccgaattccc tcggccgtgg gcagggtgcc cagtttcacg ccgttctcat
     1501 cgataatcag gacttctcgt gaggtaattt cccggttcac tcggggttct ccatcgtctt
     1561 ttcgtgttac tatagccttt cacctccggt agaaatataa aaggggccga tggaaaacca
     1621 ccggccccgc atacgcgaag acttctcttc aaaccaggcg aaatagcgct ccgcaaggtg
     1681 agagggtggc ccctcttctt ctcaatattt ttaagctaag gcattatagc acgaacaccc
     1741 gaagtacgca aattctattt ttcttccttc gtctctcccc cgcttctctg cgcattcgct
     1801 cctctgcttg aaaagaggtg aaaggcgaga atgagcgcaa agaatccgag acttgatatc
     1861 ctgaggactc tcagcggatt gaccatcagg attcccgcgg cgacgaagag cgctcgctcc
     1921 cagaattcca tctcccgccg cagcattccg atgattccgc acgagagcga gaaaatgccg
     1981 atgatcgcta tggacagatc cagaacgtat ttcgtcagaa cgaagtcgac gaacagaaga
     2041 tccggattgt agacaaagag gtacggaatc aacaacccgg aaacggccag cccaagggcg
     2101 gacatcgcaa ctttgaacgg acgcgctccg gcaagaccgg cggctgtgta gcttgtcagg
     2161 gcgacaggcg gtaccaccgc ggacatggta ccgtaataga acgcgaagaa gtgcgcggca
     2221 agcgggggga ctcccatctg ctgcagggcg ggggcggcaa tggtggcggt gatgatgtag
     2281 catggcgtcg caggcagccc catgccgagg atgatggcgg cgatcatgca gaggatgagc
     2341 gccgcccaga gcttgccgcc tgcgaacatg atgatgttca tcgcaatgac ctgccccaag
     2401 cccgtcattc caactgcgcc gacgatgaag cccactatcg cgcacgagat accgactggc
     2461 gcagcgctcc tcgttccctc atcgagggct cgaactacat ctttcagccc catgcgcgta
     2521 gactttttaa gcgaggaaac cgcaataatt gcgaagagtc cgagaaacgc ggaatagagc
     2581 ggcgtatacc ccgccacaag aaaataaatg atgacggcga gagggatgat catatgcccc
     2641 ttgtcgagca tcgttgcctt cagcgagggg atc
//
LOCUS       DAOPKU010000421         2670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_43810_length_2670_cov_3.693308, whole genome shotgun sequence.
ACCESSION   DAOPKU010000421 DAOPKU010000000
VERSION     DAOPKU010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43810_length_2670_cov_3.693308"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..665
                     /locus_tag="PK849_11685"
     CDS             126..665
                     /locus_tag="PK849_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HPE66831.1"
                     /translation="MAKPKIASVWLEACAGCHMSFLDIDERIVDLLDKVELTATPITD
                     IKEFPEVEVGVIEGALGNEEELHLAKELRERCKILIAWGDCAVFGGINCMRNFMPAEQ
                     VLREGYVETVSTVNPEGVIPGKDLPALLPYAIPVDRAVKVDVYVPGCPPDADTIYYVF
                     SEILEGRIPSVPTDIMRYD"
     gene            680..2110
                     /locus_tag="PK849_11690"
     CDS             680..2110
                     /locus_tag="PK849_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HPE66832.1"
                     /translation="MENKKNTIVINPITRIEGHGKITVDLTDDGAVDNVRFHVTQYRG
                     FEVFSKGRDFREMPVITPRICGICPVSHHLASAKACDAILGVTPPAPAAKLREVMHMG
                     QFIQSHALSFFHLSSPDLLFGFDADPAVRNVAGLAKEYPELAVAGIGMRKFGQEVIKT
                     LGGKKVHPWHSIPGGVNRSLRAEEKDAMLAGLPAHKENLRKALSLVKNYLAENAELAA
                     RFATLETSYLGLVKDGNLELYDGRIRLRSPRGRITDEFDDKDYCTYIGEHVEEWSYLK
                     FPFYRPLGFPHGSYRVGPLGRLNACEDIPTPEASAELAAFRKTTEDGIVHYTLYYHYA
                     RLIEILYAMERLEELLLDPDVLKSDLHITGEVSNREGIGVVEAPRGTLIHHYEVDENG
                     VIERVNLIVATGHNNYAMNRGVEQVARDYITGPDVKEGALNRMEHVIRCYDPCLSCST
                     HAVGKMPLLLEIRNSKGELVRTVTKE"
     gene            2107..2604
                     /locus_tag="PK849_11695"
     CDS             2107..2604
                     /locus_tag="PK849_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPE66833.1"
                     /translation="MRVFVCGYGNPFRTDDGAGHHLAPLLAGFLESCGQDVELRLEHQ
                     LLPEMAEEFAAFDLLLFADARVPGTAPGEGFLLEEIDPDPVLEGLNIHSVGPEWVLAL
                     AAQIGGKVPPALLLSVEGESFDFGEIPTPSCALRIREAAEAFKRWFRQKYPRSGCSNG
                     AQTGI"
BASE COUNT          574 a          761 c          807 g          528 t
ORIGIN      
        1 atgcagaagg cctccgccgc tccctgcgag aatgtctacg aggcgaaggc tgccccggac
       61 acggcccgaa cggtccgctt cgctccggga gcttctgtac cctttgtgaa ggaggtacga
      121 tgattatggc gaagccgaaa atcgcctcag tatggcttga agcctgcgcg gggtgccaca
      181 tgtcctttct cgacatagac gagcgcatcg tagaccttct ggacaaagtg gagcttaccg
      241 ccacgcctat taccgacatc aaggagtttc cggaagtgga ggtgggcgtt atagaaggag
      301 cgctcggcaa cgaggaggag ctgcacctgg cgaaagagct ccgcgaacgc tgcaagattc
      361 tcatcgcatg gggagactgc gccgttttcg gaggcatcaa ctgtatgcgc aactttatgc
      421 ccgccgagca ggtgctcagg gaaggatatg tggagacggt cagcacggtg aaccccgaag
      481 gggtcatccc cgggaaagac ctccccgcac tgctcccgta cgcaattccc gtggaccggg
      541 cggtgaaggt ggacgtctac gttccggggt gccctcccga cgccgacacc atctattatg
      601 tcttcagcga gattctggaa gggcggattc cctccgtccc gacggacatc atgcgctacg
      661 attagcggag gaccgaagaa tggagaacaa gaaaaatacg atagtgatca atcccattac
      721 ccgcatcgag ggccacggca aaattaccgt ggacctcacg gacgacggag cggtggacaa
      781 tgtccgcttc cacgtgacgc agtacagagg gttcgaagtt ttttccaagg gtcgcgattt
      841 ccgcgagatg cccgttatca cgccgcgcat ctgcggtatc tgcccggtga gccaccatct
      901 tgcgtccgca aaagcatgcg acgcaatcct cggcgtgacg cctcccgctc cggcggcgaa
      961 gctccgcgag gtgatgcaca tggggcagtt catccagtcc catgcgctct ccttcttcca
     1021 cctctcgagt cccgatctcc ttttcgggtt cgatgccgac ccggctgtac gaaatgtagc
     1081 cgggctggcc aaggagtacc ctgaactcgc cgtggccggg atcggcatga gaaagttcgg
     1141 acaggaggtc atcaaaaccc tcggaggcaa gaaggtccat ccgtggcaca gcatcccagg
     1201 aggagtcaac aggagcctcc gcgcggagga aaaggacgcc atgctcgcag ggcttccggc
     1261 gcacaaggag aacctgagaa aggcgctctc gctggtcaag aactacctgg ccgagaatgc
     1321 cgagcttgcc gcgcgcttcg ccacgctgga gacctcctac ctgggcctcg tgaaagacgg
     1381 caaccttgaa ctgtacgacg gacgcatccg cttgcggagc cccaggggac gcatcaccga
     1441 cgagttcgat gacaaggatt actgcactta catcggcgag cacgtcgaag agtggagtta
     1501 cctcaagttc ccgttctaca ggccgctcgg gtttccgcac ggttcttaca gggtcggccc
     1561 tctcggacgt ttgaatgcct gcgaggacat cccgacgccg gaggcctccg ccgaactggc
     1621 ggctttcagg aaaaccaccg aagacggcat tgtccactat acgctctact accactacgc
     1681 tcgcctcatc gagattctgt acgcgatgga gcggctggag gaattgctgc tcgaccctga
     1741 cgtactgaaa agcgatctgc atattacggg agaggtctcc aatcgcgagg gaatcggagt
     1801 cgtcgaggcg cctcgcggta cgctcatcca tcactatgag gtagatgaga acggagtgat
     1861 cgagcgggtg aatctcatcg ttgccaccgg gcataataat tacgcaatga acaggggtgt
     1921 agagcaggtg gcgcgtgact atattaccgg tcctgacgtc aaggaggggg cgctcaaccg
     1981 gatggagcat gttatccgtt gctacgatcc ctgcctttca tgttcaaccc atgccgttgg
     2041 caagatgccg ctgttgctcg aaatccggaa cagcaaggga gaacttgtga ggacggtcac
     2101 gaaagagtga gggttttcgt ctgcgggtac ggcaatccgt ttcgaacgga cgacggagcg
     2161 gggcatcatc ttgccccgct ccttgccggt tttctcgagt cttgcgggca ggatgtcgag
     2221 ctacggctcg aacaccagct tcttcccgaa atggcggaag agtttgccgc attcgacctg
     2281 cttctcttcg cggatgcgag agttccggga actgcacccg gcgagggatt tttactggaa
     2341 gagatcgatc ccgatccggt gctagaagga ttgaatatcc actccgtagg ccccgaatgg
     2401 gtgctcgcgc tcgcggcgca aatcgggggg aaggtccctc ccgctcttct gctgagtgtg
     2461 gagggggaat cgttcgattt cggcgagatt ccgacgccaa gctgcgctct tcgtattcgc
     2521 gaggcggcgg aggcatttaa gagatggttc cggcaaaaat accctcggag cggatgttca
     2581 aatggcgccc aaaccggtat ttgatttctc ccctcgtttg cagacggttc agtaaagaaa
     2641 aggggcggca ggttcctgcc gcccctatat
//
LOCUS       DAOPKU010000422         2663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_43956_length_2663_cov_4.317485, whole genome shotgun sequence.
ACCESSION   DAOPKU010000422 DAOPKU010000000
VERSION     DAOPKU010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43956_length_2663_cov_4.317485"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK849_11700"
     CDS             <1..399
                     /locus_tag="PK849_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPE66834.1"
                     /translation="NQPLLRPEGLAGEKILVAVVGGSSKGLGAAGIDKDQEIERVTGL
                     FQSAKQKGMKLLIMHIGGEGRRGTLSDAFIEAAAPHADRLIVVDGGNNDKIFDRLVEG
                     KNVKILTAPNVNGTAEPLKATLSEWGVSAQ"
     gene            415..1701
                     /locus_tag="PK849_11705"
     CDS             415..1701
                     /locus_tag="PK849_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE66835.1"
                     /translation="MEWFWPEGFYTIVMVGSFVLGAFALKLPIAIALSGAAVVGALAG
                     GEWFPLRHFVEGMFGYLDTILIIASAMIFMKSVQKTGLLESLAAWVIRRFRRKPLLSV
                     GLIFIIMAPGMITGSSTAAVLTTGALVAPVLIKLGVPAVKTAAAIAMGAIYGMIAPPI
                     NIPAMIIGGGIDMPYVGFALPLLVCTIPLAIFSALLLVYPSLKSGAGDEDELENELRR
                     MEETPLSARLLLPVVLLAVLMTGEQLLPSVWPALGMPVNFLLASFSGLITGRRFNFLD
                     ASTEALNEAIPVLGILMGVGMFIQIMTLIGVQGFLVVSVLALPAWLMYLGMATSIPLF
                     GAVSAFGAASVLGVPFLLALLGRNEIMVGSALSLLAGLGDLMPPTALAGIFAAQVVGE
                     ENYFKVLRYCLIPALVTALWAVFVILNANTIMGFLP"
     gene            1715..1858
                     /locus_tag="PK849_11710"
     CDS             1715..1858
                     /locus_tag="PK849_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66836.1"
                     /translation="MTLIYRAIVLFVLLLTVRCQFREKDFWKQATAALVVVPLVLRLL
                     MIK"
     gene            1874..>2663
                     /locus_tag="PK849_11715"
     CDS             1874..>2663
                     /locus_tag="PK849_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPE66837.1"
                     /translation="MKLKGTPLTAVLLLLAAFAVAALAGLNFKAMWAEDDFVPAQGFE
                     KKMLSAWFEGLAGTPADTPVYIQKGTEPGGVVLILGGTHPTEPASMVTATLFLERAQV
                     TKGSLIVIPQANLLGFTHNSPQEAHPQSISFPTADGGKRTFRYGSRLTNTIYEWPSPD
                     IYIHPASGQQLPGKERGNLNRCYPGVPDGSITEQVAYALTELVRRERVDLAFDLHEAS
                     PEYPVVNAIVAHERSMELAAMTTMDLEAAGIMMRLEPSPKNLRGL"
BASE COUNT          484 a          763 c          806 g          610 t
ORIGIN      
        1 aaccagcctc tgctcaggcc tgaaggtctt gccggcgaga aaattctcgt agccgttgtc
       61 ggaggaagct ccaaggggct cggcgcggcg ggcatcgaca aggaccagga gattgagcga
      121 gtgacgggac tcttccagtc ggcgaagcag aaaggaatga agctcctcat aatgcacatc
      181 ggcggcgaag ggagaagagg gacgctctcc gacgctttta tcgaagctgc cgctcctcac
      241 gccgacaggc ttattgtggt cgacggcgga aacaacgaca agattttcga tcgtttggtc
      301 gagggaaaga atgtcaaaat tctcaccgcg cccaatgtga acggcacggc ggagcctctc
      361 aaggcaaccc tctcggagtg gggggtatcc gcccaatagg cgggaggccc tgtcatggag
      421 tggttctggc ctgaaggatt ctatacgatc gtgatggtgg ggagctttgt tctcggcgcg
      481 tttgcgctga agctccccat agcgatcgca ctctccggcg cggcggtcgt cggcgctctc
      541 gccggcggcg aatggttccc tctccgccat ttcgtggagg gtatgttcgg ttatctggat
      601 acgattctta tcatcgcctc cgcgatgatc ttcatgaaat cagtgcagaa gacggggttg
      661 ctggagagcc tcgcggcgtg ggtaatccgc agattccgcc gcaagccgct tctcagcgtc
      721 gggttgatct tcatcatcat ggcgcccggt atgatcaccg gttcgtcgac cgcggccgtt
      781 ctcaccacgg gagcccttgt cgctcccgtt ctgataaaac tcggcgttcc ggccgtcaag
      841 actgccgcgg cgatcgcaat gggcgctatc tacggtatga tcgcaccgcc tatcaacatt
      901 ccggcgatga tcatcggcgg cggcatcgac atgccgtacg tcggctttgc cctgcctctg
      961 ctcgtctgca caattcccct ggcaatcttt tcggcgctgc tcctcgtcta cccctccctg
     1021 aaaagcggcg ccggcgatga ggatgaactg gaaaacgagc tgcggcggat ggaagagacg
     1081 cctctctcgg cgcgtctttt gcttcctgtt gtactcctgg cggtgctgat gaccggagaa
     1141 cagcttcttc cctctgtctg gcccgcgctc ggaatgccgg tgaacttctt gctggcgtct
     1201 ttttcgggat tgatcaccgg gagaaggttc aattttctcg atgcgtcgac cgaggcgctg
     1261 aatgaggcga taccggttct cggcatcctt atgggggtcg gcatgttcat ccagatcatg
     1321 acgctgatcg gcgtccaagg attccttgtc gtctccgtgc tcgcccttcc ggcgtggctt
     1381 atgtaccttg ggatggcgac gtcgatcccg ctcttcgggg ccgtctcggc gttcggcgcc
     1441 gcgtcggtgc tcggcgttcc cttcctgctg gcgcttctgg gaaggaacga aataatggtt
     1501 ggatcggcgc tcagtctcct cgcaggcctg ggcgatctca tgcctcccac tgcgcttgca
     1561 ggtattttcg cagcccaggt tgtcggggag gagaactatt tcaaggtgct tcgctactgc
     1621 ctgatcccgg cattggtaac ggcgttgtgg gcggtgttcg tcatcctcaa cgcgaacacg
     1681 atcatgggct ttctgccgta aggaggagaa aactatgact ctgatctatc gtgcaatcgt
     1741 tctttttgtc ctcctcctga cagtgcgctg ccagttcaga gagaaggatt tctggaagca
     1801 ggcgacggct gctctggttg ttgtccctct ggtattgcgt ctcttgatga tcaagtaggg
     1861 gagggtgaag acaatgaaat tgaaaggtac tccgttgaca gccgttcttc tcctccttgc
     1921 cgcgttcgcg gtcgcagcgc ttgccggact gaacttcaag gcgatgtggg cggaagatga
     1981 ttttgttccg gcgcagggct tcgagaagaa aatgctctcc gcctggtttg aagggctggc
     2041 gggaactccc gcggatactc ctgtctacat tcagaaggga acggagcctg gcggcgttgt
     2101 gcttatcctg ggcggaaccc atccgacgga gcctgcctcc atggtcaccg ccacgctctt
     2161 ccttgagcgg gcgcaggtga caaaaggaag ccttatcgtc attcctcagg cgaatctgct
     2221 ggggtttacg cacaactctc cccaggaagc gcatccgcaa agcatctctt ttcccactgc
     2281 ggacggcggc aagagaacgt tccgatatgg gtctcgtctg accaatacga tctacgaatg
     2341 gccctcgccg gacatttata ttcacccggc ttcggggcag cagcttccgg ggaaagaacg
     2401 gggtaatctc aaccggtgct acccgggagt tccggacggc tctatcaccg aacaggtcgc
     2461 gtacgcgctc acggagcttg ttcgcaggga gcgcgtcgat ctcgcgtttg acctccatga
     2521 ggcctccccg gagtaccccg tcgtgaacgc catcgtcgcg catgagcgca gtatggagct
     2581 tgccgccatg acgacgatgg atctggaagc cgcgggtatt atgatgcgcc ttgaaccctc
     2641 gccgaagaac ctccgggggc tga
//
LOCUS       DAOPKU010000423         2661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_44008_length_2661_cov_3.717191, whole genome shotgun sequence.
ACCESSION   DAOPKU010000423 DAOPKU010000000
VERSION     DAOPKU010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44008_length_2661_cov_3.717191"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PK849_11720"
     CDS             complement(<1..339)
                     /locus_tag="PK849_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66838.1"
                     /translation="MHYIALTGGGMLIVGCLLPWISLGTILVQQASDTVFVALALLSG
                     LLATGVAIHNIVKKQNRMKYAYLAIGGICLMIGLYGLLRVRDIRTTAEFANVLQKRIT
                     AEADMPVATGI"
     gene            complement(379..2079)
                     /locus_tag="PK849_11725"
     CDS             complement(379..2079)
                     /locus_tag="PK849_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPE66839.1"
                     /translation="MGNRTGILNRILSAVCLLLLLAGAALAAPSTLRVRQAYLDYPSL
                     TVYADLLDASGKPFVPGGAEQLTATVGGRAFDVSRWSAFSAKNDGVLIIFLVDTSASI
                     GQSRFTELQETVSGWIRRMGPSDRAAVMSFGENVSVVQGFTAEQNALLYAVQSLSPKD
                     PGAQLNLGLLRALELAKIKSSGLPARRMILLCSDGFRETPGGATADEVRNALASGPVP
                     VYSIFFDSPERESTNRNTEAQQLLNELGYDAGPVDGKLGPLTVAAIRVFQQSRGVAWD
                     GSVSKELLGVLRTMAKKQKDAALASIGEFSRRSGGQLYRAGTSPFTSIFDSVAASLDN
                     ALALHITLEGLVPDATMKRLEITFFDGTRSLSDGIEFRLFGAQAERMPVMLPEETKES
                     SATITDYSVWAYLLAIAAIITLPLSVWLIQKRMRTAKRESAAPDSSEKQSILPKDESS
                     PTIVLSEAAQRASLHVELTPSGARGTDTTLKAVITDRIVLGRNRGPSALSILGDATVS
                     ARHCELVFSKGRLFVNDLQSSNGTMVNGVPIQGHYPLEDGDRLTIGKTELRIRILEVK
                     "
     gene            complement(2055..2645)
                     /locus_tag="PK849_11730"
     CDS             complement(2055..2645)
                     /locus_tag="PK849_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPE66840.1"
                     /translation="MTIKRCPNGHFYDPDVNVSCPWCAVPADDNERTRRINPAAIPVA
                     GNAGSTVKLSPRNTPSSGSSIDEGHTVAVVKRKTGIDPVVGWLVCVKGPDRGRDYRIR
                     SEKNSVGRADSMDICISGDDTISRNDHAFVVYDPRKKIFRIQAGLSRGLVYLNGEEVI
                     ASELLAAYDTISVGQSELLFIPLCGERFQWETEQES"
BASE COUNT          508 a          718 c          845 g          590 t
ORIGIN      
        1 gattcccgtc gcgaccggca tatctgcttc tgcggttatc cgtttttgca gtacattggc
       61 gaactctgct gttgtgcgaa tatcacgtac acggaggagg ccgtagaggc cgatcatgag
      121 gcagatgccg ccgatggcca ggtaggcgta tttcatcctg ttttgctttt ttactatgtt
      181 gtgaatggca acccccgtcg cgaggagtcc agagagaagt gcgagcgcta cgaagacggt
      241 atccgacgcc tgctgaacga gaatcgttcc cagcgagatc cagggcagga ggcagccgac
      301 aataagcatt cctcctcctg tcaacgcgat gtagtgcatg atgcgtcatc tccctttcga
      361 tagtgtacga aaaaacggtt atttcacctc aagaatgcgg atgcgaagct ctgtcttccc
      421 tattgtcagt ctgtcgccgt cctccaaagg gtagtgtccc tgtatgggga cgccgttgac
      481 catcgttccg ttggaggact ggaggtcgtt cacgaagagt ctgcctttgg aaaaaacgag
      541 ttcgcagtgc cttgctgaga cggttgcatc gccgagtatg gagagcgcgg acggccccct
      601 gtttctgccg aggactatcc tgtcggtgat gacggccttg agagtcgtgt cagttcccct
      661 ggctccggaa ggtgtcagct caacatgcag tgaagccctc tgcgctgcct ctgaaaggac
      721 gatggttggg ctgctttcgt cttttggcag gatcgactgt ttctcggagg agtccggggc
      781 tgctgactct ctcttcgcgg tacgcatcct tttttgtatc agccaaacag agagaggaag
      841 agtgatgatc gcagcgattg cgaggaggta ggcccacact gaataatctg taatcgttgc
      901 ggaggattcc ttagtttctt cgggaagcat aactggcatc cgctcggcct gagcgccgaa
      961 cagacggaat tcgatgccgt ccgaaaggga gcgcgtgccg tcgaaaaatg taatttcgag
     1021 ccgtttcata gttgcgtcgg gaacgagccc ctcgagcgtg atatgcaggg cgagagcgtt
     1081 gtccagggat gcggcgacgg agtcgaagat ggacgtgaag ggagaggtcc ctgcgcggta
     1141 gagctgtccg ccggagcgcc tggagaactc ccctatgctt gccagcgcgg cgtccttctg
     1201 ttttttcgcc atcgtccgaa gcactcccag cagttctttg gacacggagc cgtcccatgc
     1261 gactcccctg ctctgctgaa aaacccggat ggccgcgacc gtgagcggtc ccagttttcc
     1321 gtcgacgggc ccggcgtcgt accccagttc gttcaaaagc tgctgcgctt cggtgtttct
     1381 gttggtactt tcgcgttcgg gcgagtcgaa gaagatggag tacaccggaa cagggccgga
     1441 ggcgagcgcg ttgcggactt cgtcggctgt ggcgcctccc ggcgtctcgc ggaagccgtc
     1501 gctgcacagg aggatcatcc ggcgtgccgg gagtccggag ctttttatct tcgccagttc
     1561 gagggcgcgg agaaggccga gattgagctg cgcgcctggg tctttcggtg aaagagactg
     1621 gacggcgtac agcagcgcgt tctgttcggc ggtgaagccc tggacgacgg agacgttctc
     1681 gccgaacgac atcacggccg cgcggtcgga ggggcccatg cggcggatcc agccggagac
     1741 cgtctcctgc agctcggtga agcggctctg tcctatggag gcggaggtat cgacgaggaa
     1801 gatgataagg actccgtcgt tcttcgcgga aaaagcgctc cagcggctca catcgaaggc
     1861 gcgtccgcct acggtcgcgg taagttgctc ggctccgccg gggacaaaag gttttccgga
     1921 ggcgtcgaga aggtcggcgt acacggttaa agaaggatag tcgaggtacg cctggcggac
     1981 tctcaatgtc gagggtgcgg cgagcgcggc gcctgcaagc aaaagaagca agcagactgc
     2041 ggagaggatg cggttcagga ttcctgttcg gtttcccatt ggaagcgctc cccgcacaaa
     2101 ggaatgaaaa gaagttcgct ctgcccgacc gagattgtgt cataggcggc gagcagctcc
     2161 gaggcaatga cctcttctcc attgagatag accagccctc tgctcaatcc cgcctggatt
     2221 ctgaagatct tctttctggg gtcgtagacg acgaatgcat ggtcgttccg cgagattgta
     2281 tcgtctccgg agatgcagat gtccatggag tcggccctgc cgacagagtt cttctccgaa
     2341 cgtattcggt agtcgcgtcc gcggtccggc cccttgacgc agacaagcca gccgacgacc
     2401 ggatcgattc ccgtcttgcg cttgacgacg gcgacggtat gcccttcgtc gatggagctt
     2461 ccggacgaag gtgtgtttcg gggagacagt tttacggtag accctgcgtt gcccgccacc
     2521 ggtatcgctg cggggttgat tcgccgcgtt ctttcgttgt cgtcggcggg aactgcgcac
     2581 catggacagg agacattgac gtcggggtcg tagaagtgtc cgttcgggca tcgtttgatc
     2641 gtcatttcga atcactccgt t
//
LOCUS       DAOPKU010000424         2653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_44203_length_2653_cov_4.537721, whole genome shotgun sequence.
ACCESSION   DAOPKU010000424 DAOPKU010000000
VERSION     DAOPKU010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44203_length_2653_cov_4.537721"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1813)
                     /locus_tag="PK849_11735"
     CDS             complement(143..1813)
                     /locus_tag="PK849_11735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE66841.1"
                     /translation="MKKNLLRCELDTAFLHGSVVTVNEKEEVAEALGIAGNRIAYVGN
                     DRGIEPFISENTRVVDLAGRTLMPGINDTHFHPILTGLLGAHHDSGMIDTTRVRCASL
                     AGMLGMLREAVGRKRPGEWISMMGYEPELFTDERRHPTIEELDALAPENPVHCMHGGG
                     HICMYNTKALAYLDVHIPEDAGKYPPDEVEVVDGRLTGMVRGHTHFKLWGMVGYTESQ
                     QAAAAMKSHRRALEAGITSIGDMGECGPVSYHIMQKLCRDRTFKIRVNMALHSIFGKS
                     YSLNENEHWLQLGFMSGLGDERFRVGPCKFMIDGGSGGPSCATREPYSHDPDMVRERG
                     WEREETARYIERIVGAECQCTAHAIGDLAVEFMVEGYERCFRKDPEKFRRMRHRIEHC
                     SLTDQDLIDRMARMNICPSVNAGMIQAIGANYMKFYGERNAYMAALRSMFDAGVMCSL
                     SSDAPSGPIGIQTIDGAVNRYDRNQNVQCDKSQAISVTEAIRAATYNGAYSSFEEHLK
                     GSLEVGKLADMIVLSDDILKIDPMDIHTLRVDCTVIDGLFEYERAEEA"
     gene            complement(2210..>2653)
                     /locus_tag="PK849_11740"
     CDS             complement(2210..>2653)
                     /locus_tag="PK849_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPE66842.1"
                     /translation="QIQGVGVAAVLHDLGKIDIPAEILSKPSRLKEKEYSLIQDHCVA
                     GYEILKNVDFPWPVAQAVLQHHERMDGSGYPNSLSGDDILLEARILAVSDVVEAMSSH
                     RPYRPSLGLEQGLAEVAMYRGSRFDERVVDACMAIFARGESPWTE"
BASE COUNT          446 a          829 c          804 g          574 t
ORIGIN      
        1 aacctgtcgc acttctcgta gttgcgcccc ggaggatttt ccgtgtcgat cttcaaaaag
       61 tcgctgagaa acgcctccat gcgcggtcgc aacgcctccg ccgacgcgcg gattctttcg
      121 atcatggcgc tttccatccc gcttacgcct cctccgctcg ttcgtattcg aacagcccgt
      181 cgatgacggt gcagtccacc cgaagcgtat gaatatccat agggtcgatc ttcagaatat
      241 cgtccgagag gacgatcatg tcggcgagct ttcctacttc caggctcccc ttgagatgct
      301 cctcgaacga ggagtaggcg ccgttgtacg tcgcggcgcg gatcgcctcc gtcacggaga
      361 tcgcctggct tttgtcgcac tggacgttct ggttgcggtc gtagcggttc accgccccgt
      421 cgatggtctg aatgccgatg ggaccggagg gcgcgtcgct cgacagcgag cacatgacgc
      481 ccgcgtcgaa catgctgcgg agagccgcca tgtacgcgtt gcgctccccg tagaacttca
      541 tgtagttggc cccgatcgcc tggatcatgc ccgcgttgac ggaggggcag atgttcatgc
      601 gcgccatgcg gtcgatcagg tcctgatcgg tcagcgaaca gtgctcgatg cggtggcgca
      661 tcctgcggaa tttttcgggg tctttcctga aacagcgctc gtacccctcg accatgaact
      721 cgacggccag atcgccgatg gcgtgcgcgg tgcactggca ctccgcgccg acgatccgct
      781 cgatatagcg cgccgtctcc tcgcgctccc agccgcgctc gcgcaccatg tccgggtcgt
      841 gggagtaggg ctcgcgggtc gcgcaggagg ggccgcccga ccctccgtcg atcatgaact
      901 tgcacggccc cacgcggaac cgctcgtcgc cgagtccgga catgaagccg agttggagcc
      961 agtgttcgtt ctcattcagg gagtagctct tgccgaagat gctgtggagc gccatgttga
     1021 cgcggatttt gaaggtccgg tcgcggcaga gcttctgcat gatgtggtac gagaccgggc
     1081 cgcattcgcc catgtcgccg atggaggtaa tgcccgcttc cagagcccgc cggtgcgatt
     1141 tcatcgccgc cgcggcctgc tgcgattcgg tgtagccgac catcccccac aacttgaagt
     1201 gcgtgtgtcc gcgcaccatt ccggtgagcc ttccgtcgac gacctcgact tcgtccggag
     1261 ggtactttcc cgcatcctcg gggatgtgca cgtcgaggta ggcgagcgcc ttggtgttgt
     1321 acatgcagat atgcccgccg ccgtgcatgc agtgcaccgg attttcgggc gccagggcgt
     1381 cgagctcttc gatagtgggg tgccgtcgct cgtcggtaaa gagctcgggc tcgtacccca
     1441 tcatggagat ccattctccg ggccgcttgc gtccgacggc ctcccggagc atgccgagca
     1501 tcccggccag ggaagcgcag cgcacgcgcg tcgtgtcgat catccccgag tcgtggtgtg
     1561 cgccgagaag gccggtgagg atcggatgaa agtgcgtgtc gttgattccg ggcatgagcg
     1621 tccgtccggc aagatcgacg acgcgggtgt tttcggagat gaacggctcg atcccccggt
     1681 cgttcccgac gtaggcgatg cggtttcccg cgatcccgag cgcctcggca acctcttctt
     1741 tctcattgac ggtgacgacg cttccgtgga gaaacgccgt atccagttcg cagcgcagta
     1801 aatttttctt catcggaccc actccttatt tcggaccgaa tccggagact ctcccgggag
     1861 gatttcagcc gtttcgccga gagagactcg gaataattcc gcattatgga atagcgttgc
     1921 tctatatgga ctattgaaag cattgtagcc gtaccgcctg ttttgtcaat actttctctg
     1981 cgacgcaaaa cattgataat tttttaacaa cttactcacc gaggcgcctg tttctttccg
     2041 tatgcggagg cttcgcggaa cttccgcagc gttccagctc cgggaacgaa gcaggttccg
     2101 ggcgtcattc cccgcctcct cgcaatacac aagcgcccct cttcccggga gggaaaaagg
     2161 gcgctcatta tcagcgtttg cagcatctcc gctttcttcg cggcgcgttc tactccgtcc
     2221 agggggactc tcctctggcg aagatggcca tacaggcgtc cacgaccctc tcgtcgaagc
     2281 gcgatcccct gtacatagcg acttcggcga gcccctgttc gaggccgagg ctcggccggt
     2341 aggggcggtg ggaggacatt gcctccacca cgtcggagac ggcgaggata cgcgcctcca
     2401 gcagaatgtc gtctcccgag aggctgttcg ggtatccgga tccgtccatc cgctcgtggt
     2461 gctggaggac ggcctgggcc acgggccacg ggaagtcgac gttcttgagg atttcatatc
     2521 cggcgacgca atggtcctgt atgaggctgt actccttctc cttcaggcgg gagggcttgc
     2581 tcagaatctc cgccgggatg tcgatcttcc cgagatcgtg gagcacggcg gcgacgccca
     2641 ctccctgtat ctg
//
LOCUS       DAOPKU010000425         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_44249_length_2651_cov_4.122111, whole genome shotgun sequence.
ACCESSION   DAOPKU010000425 DAOPKU010000000
VERSION     DAOPKU010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44249_length_2651_cov_4.122111"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PK849_11745"
     CDS             <1..613
                     /locus_tag="PK849_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66843.1"
                     /translation="QDKESFILEGTISGPFRTYMSLNSAVVTSVWNERLLGGAYVPSV
                     SEHHGMTLYFKPDDAARSRNGEVPAASGREVLGALMEAWHSRRGDIANIVVAASTPFS
                     FEETVQLTGFCLQNRVRIFVFADSLPSVELLRAASLTGGRETFVPNGTWGPFPAYSSG
                     ALRIVLPADETSSGFPSRSTLSVYLDIGVVPTRDWVPLWPDLL"
     gene            666..2333
                     /locus_tag="PK849_11750"
     CDS             666..2333
                     /locus_tag="PK849_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPE66844.1"
                     /translation="MNKEYTEAKARFEAGVMKTLQKNKERKETFSTGGGLPLERLYGP
                     DMTEGTDYVKEVGFPGEYPFTRGVQSTMFRGRFWTMRQYAGFASAEESNERYRYLLAS
                     GTTGLSVAFDLPTQIGYDSDDPMAQGECGKVGVAIDSLKDMEILFDKIPLDKVSTSMT
                     INAPASVLLAMYICVGEKQGVPMSALSGTIQNDILKEYIARGTYIFPPKPSMRLITDI
                     FEFCSQNVPKWNTISISGYHIREAGSTAAQEVAFTLADGIAYVEAARSKGLDPNVFGE
                     RLSFFFNAHNDFLEEVAKFRAARRMWAKIMKERFGVTNPKAQMLRFHTQTGGSTLTAQ
                     QPENNIVRVTMQALSAVLGGTQSLHTNSMDEALALPSEKSVMIALRTQQIIAYESGVT
                     NTVDPLAGSYVVESLTNEIEEKAFEYIQQIDEMGGMLTAIEKGYVQKHIQDAAYEFQR
                     AVESKENVVVGVNKFQIEEKNKDMKLLTVDASVGERQIKKLQELKASRDNVAVSNALD
                     AIRSAALNESQNLMPLIIDAVRVYATEGEICGVLRGVFGEYTENIVL"
     gene            2384..>2651
                     /locus_tag="PK849_11755"
     CDS             2384..>2651
                     /locus_tag="PK849_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869042.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPE66845.1"
                     /translation="MDERKIRVVVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     AEQIVETVLQEDADAVGISILSGAHTFYFTKIIDMLREKDAGDVI"
BASE COUNT          644 a          652 c          754 g          601 t
ORIGIN      
        1 gcaggacaaa gagagtttta ttctcgaagg aacaatcagc gggccgtttc gaacgtatat
       61 gtccctgaac tccgctgttg tgacttccgt ctggaatgag cgccttctcg gcggagcgta
      121 cgttccttct gtttcggagc atcatggaat gacgctgtac tttaaacctg atgatgcagc
      181 ccgatcacgt aacggcgagg ttcccgcggc ttccgggaga gaagttctgg gcgcgttgat
      241 ggaagcgtgg cattccagaa ggggcgacat agccaatatt gtcgttgcgg cgagcacccc
      301 tttttcgttt gaagaaacag tccagctcac gggtttttgt ttgcaaaacc gtgtgcgaat
      361 ctttgtattc gcggacagtc tcccgtccgt agagctgctt cgagcggctt cactgacggg
      421 aggaagggag acgtttgttc caaacggcac atggggaccc tttccggcgt attcttcagg
      481 cgctctcagg atcgtgctcc ctgccgacga gacctcctcg ggatttccga gcaggtcgac
      541 gctttctgtc tatctggaca tcggagttgt tccgacgaga gattgggtcc ctctctggcc
      601 ggatcttttg tgatcggtcg gagttgaaaa gtacgcacta tcttccaaag gaggagaggt
      661 tgagcatgaa caaagagtat acagaagcga aagcccgttt tgaagcggga gtcatgaaaa
      721 ctcttcaaaa aaacaaagag cggaaagaga ctttttcaac ggggggaggt cttcctcttg
      781 aacgtctgta cggacctgat atgacggagg ggaccgatta tgtgaaggaa gtcggcttcc
      841 ccggggagta tccctttaca cgaggcgtgc agtcgacgat gttcagaggc agattctgga
      901 cgatgcgcca atatgcgggg tttgcttccg ccgaggagtc gaacgagaga taccgctatc
      961 ttcttgcttc cggcacgacg gggctctcag ttgccttcga cctccccacg caaattggtt
     1021 acgactccga cgacccgatg gcacagggag aatgcgggaa agtcggcgtt gccattgaca
     1081 gtttgaaaga catggaaatt cttttcgaca agattcctct cgacaaggta tcgacgtcga
     1141 tgacgatcaa cgctccggca agcgttctac tggccatgta catctgcgtg ggagaaaagc
     1201 agggagttcc tatgagcgct ctttccggga ccatccagaa cgacatactg aaggagtata
     1261 ttgcacgagg aacctatatc tttcccccaa aaccctccat gcgcctcatc accgatattt
     1321 ttgagttctg tagccagaac gtgccgaagt ggaataccat ttccatttcc gggtaccata
     1381 tccgggaggc cggcagcacg gcggcgcagg aagtcgcgtt tacgctcgca gatggaatag
     1441 cctatgttga agcggcgcgt tcgaaaggac tggacccgaa tgtcttcggc gaacgccttt
     1501 ccttcttctt caacgcgcac aacgatttcc tcgaagaggt tgcgaaattc cgcgctgcac
     1561 gccgtatgtg ggcgaagatt atgaaggagc gtttcggcgt gacaaatccg aaagcgcaga
     1621 tgctccgttt ccatacgcag acgggcggca gcactctgac cgcacagcag ccggagaaca
     1681 acatcgttcg ggtgaccatg caagcgctct cggctgttct tggaggaacg cagtctcttc
     1741 acaccaacag catggacgag gccctcgcgc ttccgagcga gaagagcgtt atgatcgcac
     1801 tcagaactca acagatcatt gcttacgaat ccggagtcac caacacggtt gatcctcttg
     1861 caggaagcta cgtggttgaa tccctcacga atgagatcga agagaaggcg ttcgagtata
     1921 ttcagcagat cgacgaaatg ggcggcatgc tgacggcgat tgagaagggg tatgtccaaa
     1981 agcacatcca ggatgcagcg tacgaatttc agcgtgctgt ggaatcaaaa gaaaatgttg
     2041 tcgtcggagt caacaagttc cagatagagg agaagaacaa agatatgaag cttttgactg
     2101 tcgacgcctc cgtcggagag cggcagatca agaagctgca agaattgaaa gcctcccgtg
     2161 acaatgttgc cgtgtccaac gctcttgacg ccatccgctc agccgctttg aacgagtcgc
     2221 agaatcttat gccgcttatt attgacgcag tccgtgtcta tgcgactgaa ggtgaaatct
     2281 gcggcgtact ccgcggcgtc ttcggcgaat atacagaaaa tatcgttttg tagcctttta
     2341 attaaggctg tgcatcagga ttatcataat ggagggatct tccatggatg agagaaaaat
     2401 acgggttgtg gtggcgaaac cgggtcttga cggccatgac agaggcgcga aggtcgtagc
     2461 gcgcgcgctt agagacgcag gaatggaggt catttatacc ggtcttcgcc aaaccgcgga
     2521 gcagatcgtg gagactgtgc tgcaggaaga cgccgacgca gtgggaatta gcatcctctc
     2581 cggagctcat acgttttatt tcaccaagat tatcgacatg ctcagggaga aggacgccgg
     2641 agatgttatc g
//
LOCUS       DAOPKU010000426         2645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_44396_length_2645_cov_5.125869, whole genome shotgun sequence.
ACCESSION   DAOPKU010000426 DAOPKU010000000
VERSION     DAOPKU010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44396_length_2645_cov_5.125869"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK849_11760"
     CDS             complement(<1..411)
                     /locus_tag="PK849_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66846.1"
                     /translation="MLLALCCLWSVPASAAEVFLVSSYHEGDMCGQPQYEAVVEALKQ
                     SGIPELSFRGYYLDSRRRVGKDIDREIDTIIADIRERKPALVFTIDDLAFARLYGEVL
                     RHPSTFLVFTGLNRAVAEYDKGASFLDEKGLPAKN"
     gene            complement(671..970)
                     /locus_tag="PK849_11765"
     CDS             complement(671..970)
                     /locus_tag="PK849_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66847.1"
                     /translation="MNCENSVVVSGAIHLPKGDMVGRNRHGTYLKFALKQMTTGYDGV
                     ERPSFLPVRVFDQALQEQFRAKGHGDSVRIAGTLRSSTGSGEMFILAESIENTKE"
     gene            complement(1061..1966)
                     /locus_tag="PK849_11770"
     CDS             complement(1061..1966)
                     /locus_tag="PK849_11770"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HPE66848.1"
                     /translation="MKKLLALFLAIVLMTGFVAAANAADDPMKYEGRLSIASQNVNET
                     IILAWMAKLLVEEYTGLRTTMNTEFAGSAVLHQAMVAGEIDLYSSWTGTQLTGILRYE
                     GESKSSEETFRMVKEGFEKNFGMTWTRPVGFNNTYIMAVRPETAEKYNLKKASDLAAH
                     APGWKLGGDENFDTRLDAYPGWSERYGITFRDVLPMQYAMMYMALMQEEVDVIAAYST
                     DSRIGKFNLVLLEDDKQFFPDYSAAFVLPLALTEKYPGLVGVVEKLSGKIDEKAMAAM
                     NLAFDEGENPEDIARAFLREKQLVK"
     gene            complement(2023..>2645)
                     /locus_tag="PK849_11775"
     CDS             complement(2023..>2645)
                     /locus_tag="PK849_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66849.1"
                     /translation="AALIAVGITVAICVPLGIYLTRNERAAPYVIGLANVFQTVPSLA
                     LLGFLIFVLGIGNDNAICALFLYAMLPVLQNTYTGIRGVPKSYVQAARGMGMTDFQIL
                     MKVQFPLALPVLVAGIRVAMVWTIGTATLASAIGGGGLGRLIFSGLSSIRNEIILAGA
                     IPATLLALAADWLLKRAQEYFNPSARAKRLAKRHGISAETESNSAA"
BASE COUNT          552 a          815 c          739 g          539 t
ORIGIN      
        1 attcttcgca ggaagcccct tctcatcaag gaaggaagcg cccttatcat actctgcgac
       61 cgcacggttc agtccggtga acacaagaaa ggtcgacgga tggcgcagca cctcgccgta
      121 cagccgggcg aaggcaaggt cgtcaatcgt gaacacaagg gcgggttttc gttccctgat
      181 gtccgcgatg attgtgtcga tctccctgtc gatatccttc ccgacgcgcc gccgggagtc
      241 gagatagtac ccacggaagg agagctccgg aataccgctc tgcttgagcg cctccacaac
      301 cgcttcgtac tgcggctgcc cgcacatatc cccctcgtgg tagctggaca ccaggaatac
      361 ttcagccgcc gaagccggaa cgctccacag acagcacagc gcaagaagca aaagaagccg
      421 cctcccggat ttccacagtc tctcccgaac agaagaacca agcgcacgtt tttcgttttc
      481 cgcagctgac gaaacagtca catgcacacg ctcctttcag cgcatccggc gcaccccgct
      541 aaggcgcgta ccaacctgca ataaaatcta aggaagatag cttttcatac tatacaacaa
      601 agacgccctc cgtgaaagaa ggcgtctttg tttttgttgt cattacatcg aaaaaaatca
      661 cccgtaaagg ctactccttt gtattttcga tggattccgc aaggataaac atctcgccgc
      721 tccctgtcga agagcgaagc gtcccggcta tccggacaga gtctccatgc cccttggccc
      781 ggaactgctc ctggagcgcc tgatcgaaaa cgcgcaccgg gagaaagctt ggccgttcca
      841 caccgtcata cccggtcgtc atctgcttga gggcgaactt aaggtacgtt ccgtgcctgt
      901 tgcgcccgac catgtcgccc ttgggcagat gaatcgcgcc ggaaactaca acggaatttt
      961 cacagttcaa ctacaatcca actccttaaa aaccaatctt cctacccctg tgagtagatg
     1021 aagaagatac cactttttcc ccttttccgc aaattttttt ctacttgacg agttgttttt
     1081 cacggaggaa ggcgcgtgcg atgtcctcgg ggttttctcc ctcgtcgaag gccagattca
     1141 tggcggccat cgccttctcg tcgatcttcc cgctcagctt ctcaacgacc ccgacaagcc
     1201 cggggtactt ctccgtaagc gccagaggca gaacgaacgc ggcgctgtag tcggggaaga
     1261 actgcttgtc gtcttccaga agaacgaggt tgaactttcc tatccgcgaa tcggtcgagt
     1321 aggccgcgat gacgtcgact tcttcctgca tcagcgccat gtacatcatg gcgtactgca
     1381 tcggcaggac gtcgcggaag gtgatgccgt agcgctcgga ccagccggga taggcgtcga
     1441 gacgcgtgtc gaagttctcg tcgccgccga gcttccaccc gggagcgtgc gccgccagat
     1501 cggacgcctt cttcaggttg tacttctccg ccgtctcggg gcgcacggcc atgatgtacg
     1561 tgttgttgaa gccgaccggg cgcgtccacg tcatgccgaa gttcttctcg aacccctctt
     1621 tcaccatccg gaaggtctct tcggaggact tcgattcgcc ctcgtagcgc agaatgccgg
     1681 tgagctgcgt cccggtccac gaggaataga ggtcgatctc ccccgcgacc atggcctggt
     1741 gaagcaccgc cgacccggcg aactccgtgt tcatcgtcgt gcgcagcccg gtgtactcct
     1801 cgacgaggag cttcgccatc caggcgagga tgatcgtctc gttgacgttc tgcgacgcga
     1861 tactcagccg cccctcgtac ttcatcggat catcggccgc gttggcggcg gcgacaaacc
     1921 ctgtcatcag tactatcgca agaaatagtg caagcaactt cttcatggaa ccactctcct
     1981 tcagtgtatt ttttgtttcg ttcacggcgc ggagcgcgga gctcacgccg cggagttgga
     2041 ttctgtctcg gcggaaatac cgtgcctctt cgccagccgc ttcgcccgcg cggaggggtt
     2101 gaaatattcc tgcgctcgct tcagaagcca gtccgcggcg agcgcgagca gcgtcgcagg
     2161 gatggccccg gcgaggatga tctcgttccg tatggaagag aggccggaga agatgagccg
     2221 tccgagtccg ccgccgccga tcgccgaggc gagcgtcgcc gtgccgatcg tccagaccat
     2281 ggccacccgg atgcccgcga cgagcacggg aagggcgagt gggaactgta ccttcatcag
     2341 gatctggaag tcggtcatcc ccatgccgcg cgcggcctga acgtaggact tcggaacgcc
     2401 tctgatgccg gtgtaggtat tctggagcac cggaagcatg gcgtagagga agagcgcgca
     2461 gatcgcgttg tcatttccta ttcccagaac gaagatcagg aaacccagca gggcaaggct
     2521 cggcaccgtc tgaaagacgt tcgccaggcc gatcacgtac ggggctgcgc gctcgttccg
     2581 cgtgaggtag atccccagtg gaacgcagat ggcaacggtg atgccgacgg cgatcagagc
     2641 ggcga
//
LOCUS       DAOPKU010000427         2645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_44404_length_2645_cov_4.182625, whole genome shotgun sequence.
ACCESSION   DAOPKU010000427 DAOPKU010000000
VERSION     DAOPKU010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44404_length_2645_cov_4.182625"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1717)
                     /locus_tag="PK849_11780"
     CDS             complement(56..1717)
                     /locus_tag="PK849_11780"
                     /inference="COORDINATES: protein motif:HMM:NF033578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HPE66850.1"
                     /translation="MNVYKSPVGQPKLKFEGIPFEFTPDPNNRWVRLADLIPWEELEL
                     LSGYRNHFGSTGNPALPFRVAFGALLVQARLKLTDEETVEQIRENPYIQYFLGFEGYR
                     SEKKPFDPSMMVYFRKRLDTETLKALDLRIFERRRELEEIAKKAAEEAAKKGKKEEEG
                     SSVPEPESAPVDGSDEETPPSASPETPCGSNAPTPKRTPQERPSPAKGDAAPKGTLIL
                     DATCAPADITYPTDLKILSAARIATEKLIDAVWERIGKPGEKKPRTYRRIAARNAVVA
                     CKQRNIGGKKLRSALKKQLSWLARNLNSIEDLLASSAYGENLLNERERKTLETAREVH
                     RQQKHMLDTRTHSAERRIVNFAQPHVRPIVRGKAGAKVEFGAKILVAIENGHAFVVHS
                     SWENFNESTHFVEGCVQYRERNGYWPEVVCADMIFRTRANLAWCRERGIRLSGPGLGR
                     PPLDEGKLEERKLLERKDAAKRNEVEGKFGEGKRKYGLDRIRGKLPETSDSIIILQFI
                     IMNLWRMLRDILSFFLESVEKVVLVMSWLSLIHNLATFALPKQGS"
     gene            complement(1972..>2645)
                     /locus_tag="PK849_11785"
     CDS             complement(1972..>2645)
                     /locus_tag="PK849_11785"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HPE66851.1"
                     /translation="MAVIGSNVIEFNLRTAIRKVDISGFRDILSEKCSDPAVRWFSMP
                     HTELLESARADAPLLEGGDVIVLGRTVLVGNTENPSVGSNEAGFNWLKNILGDDYTVK
                     RVRLVESVLHLDCVLSVPRDGLAIICEEAFLDGLPEEIKDWDLIRVSLDDVKRLAVNG
                     LPVNSQHYVLSYNLHNDNRYIQTELEKRGITVHRVFFGTHNGQGGSLRCATQPLKREV
                     RTTKP"
BASE COUNT          469 a          779 c          742 g          655 t
ORIGIN      
        1 tttccaacat ggcgaccatg cttcggctga acctctttag ggcatcctga acaagttacg
       61 agccttgctt cggtaaagca aaagtggcca agttgtggat aagactcaac cacgacatga
      121 ccaaaacgac tttttcgacg ctttccagaa aaaaagacaa aatatcccgg agcatcctcc
      181 aaagattcat tattatgaac tgaaggataa taatactgtc gcttgtttcg ggaagtttcc
      241 cccgtatccg gtccagaccg tacttcctct tcccttctcc gaacttgccc tcgacttcgt
      301 tcctcttcgc cgcgtctttc ctttccagca acttccgctc ctcgagcttc ccctcgtcaa
      361 gcggcggtct tccgagcccc gggccgctga gccggattcc tcgctcccgg caccacgcca
      421 ggttggcccg cgttcggaag atcatgtccg cgcataccac ttccggccag tatccgttcc
      481 gttcccggta ctggacgcat ccctcgacga agtgcgtgct ttcgttgaag ttttcccacg
      541 acgagtgaac gacaaaggcg tgtccgttct ctatggcaac gaggatcttc gctccaaatt
      601 cgactttcgc tcccgctttg cccctcacta tcggccggac gtgcggctgg gcgaagttga
      661 ctattctccg ttccgcgctg tgcgtccggg tgtccagcat gtgcttctgc tgtcggtgca
      721 cctcccgcgc ggtttcgagc gtcttccgtt cccgttcgtt caggaggttc tctccgtatg
      781 cgctcgaggc aagcaggtcc tcgatggagt tcaggtttct cgcaagccag gagagctgct
      841 tcttgagggc gctccggagc ttcttcccgc cgatgtttct ttgtttgcag gcaacgactg
      901 cgttcctggc ggcgatgcgg cggtaggtac ggggcttctt ctctccaggc ttcccgattc
      961 gttcccagac cgcgtcgatg agtttctccg tggcaatcct ggcggcagaa agtatcttga
     1021 ggtcggtggg ataggtgatg tcggcaggag cacaggtggc gtccaggatg agcgttccct
     1081 tgggggcggc gtctcccttc gcgggagacg gtctctcctg tggggttctt ttcggtgtcg
     1141 gagcgttgct tccgcagggt gtctccggac ttgccgaggg cggtgtttct tcgtcgctcc
     1201 cgtcaaccgg ggccgattcc ggttccggta cggaagagcc ctcttcttcc ttcttcccct
     1261 tcttcgccgc ctcttccgca gctttcttcg cgatctcttc caattctctc cgtctctcga
     1321 agatgcgaag atccagggcc ttgagcgttt cagtatccag acgtttcctg aagtacacca
     1381 tcatggacgg gtcgaacggt ttcttttccg agcggtagcc ttcgaagccg aggaagtact
     1441 ggatgtacgg gttctcccga atctgctcga ccgtctcttc gtcggtgagc tttagcctcg
     1501 cctgcacgag aagcgccccg aaggcgacgc ggaaggggag cgccgggttg ccggtgctcc
     1561 cgaagtgatt ccggtagccg gagaggagtt cgagctcctc ccacgggatg aggtcggcaa
     1621 gacgaaccca tctgttgttc gggtcaggtg tgaattcgaa gggaatgccc tcaaatttca
     1681 atttcggctg gccgaccggt gacttataca cattcatccc acgctctctg caaggttttt
     1741 cgggctcata tacccttttt cctgcaccat tgtaccgtga aaaccgcttc tttttcagta
     1801 atgataagac tttatgactt gttcaggatg ccctaaaggg acacccactt taccgcaata
     1861 ggtcacggct tcccgaagat acctgagggc gctccgacgc aaaggcggga aaacccccgc
     1921 aggtgctgga gaacatgttc tctccgcttg accgcaagtg cccttatagt ttcagggttt
     1981 cgtcgttctg acctcccgct tcagaggctg ggtcgcgcag cgcagcgatc cgccctggcc
     2041 gttgtgggtt ccgaaaaaca cgcggtggac tgtgatgccc cgcttttcga gctcagtctg
     2101 gatgtagcgg ttgtcattgt gcaggttata ggacagaacg tagtgttggg aattgacggg
     2161 aaggccgttg acggccagcc gcttgacatc atccaggctg accctgatca ggtcccagtc
     2221 tttgatctct tcgggcaggc cgtcgaggaa ggcttcttcg cagatgatgg cgagtccgtc
     2281 gcggggtacg gagagcacgc agtccaggtg cagcacgctt tccacaagcc ggacccgttt
     2341 caccgtatag tcatcgccca ggatgttttt aagccagttg aagccggcct cgttggatcc
     2401 cacgctgggg ttttccgtat tgccgacgag aacggttctg cccagtacga tgacgtcgcc
     2461 gccctccaga aggggcgcat cggcgcgggc ggattcgaga agctccgtgt ggggcatgga
     2521 gaaccacctg actgcagggt cggaacattt ttcggacagg atatcccgga atccggagat
     2581 gtccaccttt cggatcgccg tccgcagatt gaactcgatg acgttgctgc cgatgacggc
     2641 catgt
//
LOCUS       DAOPKU010000428         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_44887_length_2626_cov_4.479191, whole genome shotgun sequence.
ACCESSION   DAOPKU010000428 DAOPKU010000000
VERSION     DAOPKU010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44887_length_2626_cov_4.479191"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..>2626)
                     /locus_tag="PK849_11790"
     CDS             complement(160..>2626)
                     /locus_tag="PK849_11790"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPE66852.1"
                     /translation="PILRGIRKEKSFEEFARRVARTVACAETSYLEGGEEDLQWLRRL
                     ERNIFDDILNRRFLFNSPCLFGAGAGLTVDPGMAERIYCDPEELTFEDYSLLHRSKTK
                     GQQLFACFVIEVPDSIDGIFDSVKDAAVISKYGGGVGGNFGHLRERSSVINGGIGGQA
                     SGPVSFMETWNTMGAVVVQGGRRRAALMGMLFDDHPDIFDFIDAKVDEGKLPYFNISV
                     CVSDKLMKSAQTGADFNLVSRSNGSVVRSLNGAELWSKLCENAWKRGDPGIFFIDRAN
                     ADNLLKLDETWRIESTNPCGEQPLPNYTSCNLGSVNVEAFVERREDGNVRFDFAAFSD
                     QVFRSMYYLDLVIDACSYPLEKIGERTRRIRPVGLGIMGLADAAILLRMRYGSERFKV
                     FCRTLADSMGSAALCASVGMAEEMGKEPFPDAHLVEELFRRFAAEQGEELFPESWFAS
                     LDRESMQSLVEKMRSSPTIPYTLANAFESFFNAEGLGNEEERLALAAKVLQAFARGRL
                     RTSRRLSIAPTGSISMLIDASAGIEPNFAWSWSRRIAKADGDGQETREFHHKLLSPLQ
                     AAELHDTGALSDPVFVTAYDITPEEHVEVTGIFAGVVDSGISKTVNLPNDATVDQVKA
                     IYRRCYEMGAKGITIYRDGSRSFQPIETTKKPEKAEQPEEKQPLSSRVKERPGLVVFG
                     KTIKETTPWGSIYVTLNFDGNDPFEVFATIGKSGSELKAMTEALSRAISIGLRSGGKL
                     EDFIATLKGLSGKEYWLFEFDDKHVARSIPDAIAVLLERLIGKDIGTQGVSAGVECPE
                     CGAPMEMISGCEYCFSCGYSPCK"
BASE COUNT          513 a          828 c          746 g          539 t
ORIGIN      
        1 atcccttccg acgaaatagt gcgcctcata gagcggcgga acgagattct cggttttctc
       61 ttcgatatcg tcgcttaaaa acctccccct tcataataat aagatgaggc cggaagagct
      121 ctgcgctcct tccggcctca tcttattatc cggcgatcat tacttgcagg gggagtaccc
      181 gcagctgaag cagtactcgc acccggaaat catctccatc ggcgcgccgc actcagggca
      241 ctcgacgcct gcgcttacgc cctgcgttcc aatatccttt cctatcagcc gttcgagcag
      301 cactgcgatc gcgtccggta tggaacgggc gacgtgcttg tcgtcgaact cgaagagcca
      361 gtactccttc cctgaaagtc ccttaagcgt ggcgatgaag tcctccagct tgccgccgct
      421 gcgaaggccg atggaaatcg cgcgagagag cgcctccgtc atggctttca gctcagagcc
      481 gctcttgccg atggtggcga acacctcgaa cggatcgttg ccgtcgaagt tcagggtgac
      541 gtagatactc ccccagggcg tcgtctcctt gatcgtcttt ccgaagacaa ccaatcccgg
      601 gcgctctttt accctgctgg agagcggctg cttctcctcc ggttgctcgg ccttctccgg
      661 ctttttcgtc gtctcgatcg gctggaagga gcgggatccg tcgcggtaga tcgttatccc
      721 cttcgcgccc atctcgtagc agcgccggta gatcgccttc acctggtcga cggtcgcgtc
      781 gttggggagg ttcacggtct tgctgatgcc gctgtccaca acgccagcga agataccggt
      841 cacctcgaca tgctcttccg gggtgatgtc gtaggcggtc acgaagacgg ggtccgagag
      901 cgcccccgtg tcgtgcagct ccgcagcctg aagaggcgag agcagcttgt gatggaactc
      961 tctcgtctcc tgaccgtcgc cgtcggcttt ggcgatacgg cggctccagc tccaggcaaa
     1021 gttcggttcg ataccagcgc tggcgtcgat gagcatggag atcgagccgg tgggcgcgat
     1081 ggagagacgt ctgcttgtgc ggaggcgccc tctcgcgaac gcctgaagga ccttcgcggc
     1141 aagcgcaagg cgctcttctt cattgccgag cccctcggcg ttgaagaagg actcgaacgc
     1201 attcgcaagg gtgtagggaa tcgtcggcga cgaacgcatc ttctcaacaa gcgactgcat
     1261 tgattcacga tcaagggaag caaaccacga ttcggggaag agctcctccc cctgttcagc
     1321 tgcaaatctg cggaagagct cctcgacaag gtgcgcgtcg gggaacggct ccttccccat
     1381 ctcctcggcc atcccgacgc ttgcgcagag cgccgcgctc cccatggaat cggcgagcgt
     1441 cctgcagaaa accttgaaac gctccgagcc gtaccgcatc ctcagcaaga tggcggcgtc
     1501 ggccagtccc atgataccca gtcccacggg acggattcgc cgcgtgcgct ccccgatctt
     1561 ctccagcggg taggagcagg cgtcgataac gaggtcgaga tagtacatcg accggaaaac
     1621 ctgatctgaa aaagcggcga agtcgaaacg aacgttcccg tcctccctgc gctccacgaa
     1681 ggcctctaca ttcactgagc caagattaca gctggtataa tttggcagtg gttgttcgcc
     1741 gcatggattc gtagactcga tacgccacgt ctcgtcgagc ttcagcagat tgtcggcgtt
     1801 cgccctatcg atgaaaaaga tgcctggatc gccgcgcttc cacgcgtttt cgcagagttt
     1861 gctccacagt tcggcgccgt tgagcgagcg gacaacactc ccgttgcttc gggaaacgag
     1921 gttgaagtcg gcgccggtct gcgcggactt catcagttta tcggagacgc agacggagat
     1981 gttgaagtac ggaagcttcc cctcgtcgac cttcgcgtcg atgaagtcga agatgtcggg
     2041 gtggtcgtcg aagagcatcc ccatgagcgc ggcgcgcctg cggcctccct gcaccaccac
     2101 cgcgcccatt gtgttccacg tttccatgaa cgacaccgga ccgctcgcct ggccgccgat
     2161 gcctccgttg atgacggagc tgcgctcgcg aagatggccg aagtttcctc cgacgccgcc
     2221 gccgtacttg ctgatcacgg ccgcgtcctt gacggagtcg aaaatgccgt cgatactgtc
     2281 ggggacctct atgacaaagc aggcgaagag ctgctgcccc ttcgtcttcg aacgatggag
     2341 caaagagtag tcttcaaacg tcagttcctc aggatcgcaa tagatcctct ccgccatccc
     2401 ggggtccacg gtcaaacccg cccccgctcc gaagagacac ggggagttga acaggaagcg
     2461 gcggttcagg atatcgtcga agatattccg ttccagcctg cgaagccact gcagatcctc
     2521 ttcccctcct tcgagatacg acgtctccgc gcatgcgaca gtacgcgcta cccgccgggc
     2581 aaactcctcg aacgatttct ccttccggat tccccgaaga atagga
//
LOCUS       DAOPKU010000429         2623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_44964_length_2623_cov_4.267523, whole genome shotgun sequence.
ACCESSION   DAOPKU010000429 DAOPKU010000000
VERSION     DAOPKU010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44964_length_2623_cov_4.267523"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK849_11795"
     CDS             complement(<1..194)
                     /locus_tag="PK849_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002867082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HPE66853.1"
                     /translation="MFKIDLNSDLGESFGPWRMGMDCEVMEHVSSANVACGFHAGDPE
                     VMIRTVQAAKEASVAVGAHP"
     gene            complement(194..1288)
                     /locus_tag="PK849_11800"
     CDS             complement(194..1288)
                     /locus_tag="PK849_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HPE66854.1"
                     /translation="MAALAFSVQMPGMLTTVQDSGRWSYQGKGMPVAGAMDMQSFALG
                     NILVGNDENAAALEVTLLGPTLSVTEGEGVAAVTGAELGFTVNGEPIANWTVVTIREG
                     DILSFDGPRSGCRAYLCVSGGIDVPMVMESRSTYTRAKVGGLEGRALKKGDAVRCGEP
                     APLWNRCEGLVCPEALRPLRDPAAPLRVIPGPQDDLFTEKGLASFYGTEYVISNSADR
                     MGYRMDGEPIEHTGAADIISDAIPLGAVQVPGHGQPIVMLADRQTTGGYTKIGVVCSV
                     DVAALSQRLPGAKVRFSKIAVKDAVGLVRAEARTRDELRKLRAAWRSSARVSSAPVPG
                     AVAAPTGGEAVLRVDGKEHVISWEEIGEVE"
     gene            complement(1291..2061)
                     /gene="pxpB"
                     /locus_tag="PK849_11805"
     CDS             complement(1291..2061)
                     /gene="pxpB"
                     /locus_tag="PK849_11805"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HPE66855.1"
                     /translation="MNEKKYPRLLTAGDGCVVVEFGDVIDMEINSRVASLAAAVRKAE
                     FPGFLDTVPTYRSLAVYFNPVTTDVDALYSKLGKMASALGTGKGSESRRVIVVPSLYG
                     GEHGPDLADVAEHTGLTPEEVVKRHTANDCYCYMLGFTPGFAYLGGMDPSLETPRLKN
                     PRELIPAGSVGIAGKQTGIYSISSPGGWRLIGRTPMRMFDPDRNPAIFLEAGMWVRFR
                     AVGKAEFDEIEAATAARTYRPEILEEVSPRLVGAGEVL"
     gene            complement(2099..>2623)
                     /locus_tag="PK849_11810"
     CDS             complement(2099..>2623)
                     /locus_tag="PK849_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66856.1"
                     /translation="SINGIAITGVMRYLLFLAFLGVVLTGTPIDMTNPVASAFQIGAG
                     ALGFRIAGVVLWAAAITSVVGASYTSVSFLRTLFKVVDDYYRYWIIAFIVASTSILTT
                     VGRPVALLIVAGSLNGLILPLSLGCVLMAAHNKNIVKDYMHPVWMTALGWVIVVFTAW
                     MGFNSFAGIMKLFQ"
BASE COUNT          491 a          858 c          802 g          472 t
ORIGIN      
        1 caggggtgcg ctcccacggc gacggaggcc tctttggccg cctgcaccgt gcggatcatt
       61 acttcgggat cgcccgcgtg gaaaccgcac gccacgtttg cggagctgac gtgctccatc
      121 acttcgcaat ccattcccat gcgccacggc ccgaagctct cgccgaggtc gctgttcagg
      181 tcaattttga acatcattcc acctccccga tctcctccca ggagatcacg tgttcctttc
      241 cgtcgacgcg gagaaccgcc tcgccgccgg tcggcgccgc gacagctccc ggtacgggcg
      301 cagaagatac gcgcgcgctt gagcgccacg ccgcccggag tttcctcagc tcgtccctcg
      361 tccgcgcctc tgcgcgcaca aggccgacgg cgtcctttac tgcaattttc gaaaagcgca
      421 ccttcgctcc gggaagacgc tgcgaaagcg cggcaacgtc gaccgagcac acgacgccta
      481 tcttcgtgta ccctcctgtc gtctgccggt cggccagcat gacgataggc tgtccgtggc
      541 cggggacctg cacggctccc aagggaatgg cgtcggagat gatgtcggca gccccggtat
      601 gctctatcgg ttcgccgtcc atcctatacc ccatccggtc ggcgctgttg gagatgacat
      661 actccgtgcc gtagaaagag gcaagcccct tctcggtgaa caggtcatcc tgaggaccgg
      721 gaatgacgcg cagcggggct gcgggatcgc ggagaggacg cagcgcctcg ggacaaacca
      781 acccttcgca tcgattccag aggggcgccg gttcgccgca gcgcaccgca tcgccctttt
      841 taagggcgcg cccttccaga ccgcccacct tcgcgcgagt atatgtggag cggctctcca
      901 tgaccattgg gacgtcaatc cctccggaga cgcagaggta cgccctgcac ccggagcgcg
      961 gaccgtcgaa ggagagaatg tctccctcgc gtattgtcac aacagtccag ttcgcgatgg
     1021 gctcgccgtt gacggtgaaa ccgagctccg cgcccgtgac ggcggcaacc ccttccccct
     1081 ccgttacgga gagggtgggt cccagcaggg tgacttcgag agcggcggcg ttttcgtcgt
     1141 tgcctacgag gatgttgccg agcgcgaacg actgcatatc catggccccc gccaccggca
     1201 ttcccttccc ctggtagctc catcgtccgg agtcctgaac cgtggtaagc atcccgggca
     1261 tctgtaccga aaaagcaaga gctgccataa tcacaacacc tccccggccc cgaccagccg
     1321 gggcgagacc tcttccagta tttccggccg gtatgttctt gccgccgtgg ccgcttctat
     1381 ttcgtcgaac tcggccttgc cgaccgcacg gaagcgcacc cacatccccg cctccaggaa
     1441 gatggcggga ttccggtcgg ggtcgaacat ccgcatcggc gtgcggccga tcagccgcca
     1501 ccctcccggg cttgaaatcg agtagattcc cgtctgtttg cctgcgatgc ccacggaacc
     1561 cgcagggatg agctcccgag gattcttcag ccgcggcgtt tcaagcgacg ggtccattcc
     1621 gccgaggtag gcgaagcccg gagtaaagcc gagcatatag cagtagcagt cgttcgccgt
     1681 gtgccgcttg acgacctctt ccggcgtcag gccggtgtgc tccgcgacgt ccgccagatc
     1741 tgggccatgc tctccgccgt agagggaggg cacgacgatg acccggcgcg attcgcttcc
     1801 tttacctgtt cccagcgccg aggccatctt cccgagtttc gagtagagag cgtcgacatc
     1861 ggtcgtgacc ggattgaaat agactgccag cgagcggtac gtcgggacgg tatccagaaa
     1921 cccggggaat tccgccttcc gtaccgccgc ggcgagcgag gcgacgcgcg agtttatttc
     1981 catatcgatg acgtcgccga attcgacgac cacgcagccg tcgcccgccg tcagcagtct
     2041 cggatacttc ttctcattca cgcggacacc gcctccttca taaatatctt tcttcttcct
     2101 actggaacag cttcatgatc ccggcgaagg agttgaagcc catccaggcg gtgaagacga
     2161 cgatgaccca gccgagcgcc gtcatccata cggggtgcat gtagtccttc acgatgttct
     2221 tgttgtgcgc cgccatcaga acgcagccca gcgacagagg aaggatcagc ccgttgagcg
     2281 aaccggcgac gataagcagg gcgaccggac ggccgacggt ggtcaggatg gatgtggagg
     2341 cgacgatgaa cgcgatgatc cagtaacggt agtagtcgtc gacaaccttg aagagcgtcc
     2401 tcaggaagga gaccgacgtg taggacgcgc cgacgaccga agtgatggcc gcggcccaga
     2461 ggacgactcc ggcgatgcgg aagcccaggg cgcctgcgcc gatctggaag gccgaggcga
     2521 cggggttggt catgtcgatg ggggttccgg tgagcaccac gccgaggaag gcgaggaaaa
     2581 gcagatagcg cataacgccg gtgatggcga tgccgttgat gga
//
LOCUS       DAOPKU010000430         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_45017_length_2621_cov_3.777085, whole genome shotgun sequence.
ACCESSION   DAOPKU010000430 DAOPKU010000000
VERSION     DAOPKU010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45017_length_2621_cov_3.777085"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1153)
                     /locus_tag="PK849_11815"
     CDS             complement(140..1153)
                     /locus_tag="PK849_11815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPE66857.1"
                     /translation="MIRSCRTYTPPSKEGEGERILFLRYSALGDILIVTPYCRALKES
                     YPRSHVTWLAYEQYVPFLREQPYIDSVLPWDTSAGEREFFSLLRRVGRERFTRLVSFH
                     SSDRGALIALFSKTPLRFCNASKWGALYDTPRDLSFWTAHDLRIPEGSALGYVVTPDM
                     RKRAASLLGEHASRPFILGAIGASKEIKRWPVGRWKEFTAYAVRAGLQVVLAGEGEEE
                     KTAASVIETAVCSPLVRNVVGKIPLADLGGAISLSSAVVAGDTGILHIARMLDVPSFA
                     LLGPTPLPEGLGLARPERTFWTRCPLKACNNWRCTQECLGTISSSDVWNVVSAFLERR
                     SSK"
     gene            1345..>2621
                     /locus_tag="PK849_11820"
     CDS             1345..>2621
                     /locus_tag="PK849_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPE66858.1"
                     /translation="MILHSEKFKDIRIAVVGDVVLDKYVSGIARRISSEAAIPVLLAQ
                     RIGHVAGGAANVAANLSGIGCSVSVYGAVGNDGNSVLLGAILYEKKIENKLLQIDGRP
                     TTIKTRIVAGNQQIARYDIEESSALSKHDEDLLFSSLLEEMDGLDAIILSDYGNGVLK
                     GSLCSRIIGTAATKGTPVFVDPRGNNWNRYRGAACATPNEAELALVSEGAEIDEQTLS
                     LHAHSARKRYGLRNLLVTRGAKGMALFLEDGEEFFLPAFCVREVFDVSGAGDTVIAVL
                     AALLIAGANWKKAADWANKAAGVVVTRAGTSPVSLEDLLSLNQVERSGKVVSFEEATA
                     RVEDWKREGKTVVFTNGCFDLLHSGHVHLLRHAAREGDRLVVGVNSDASVQRLKGPSR
                     PVASQEDRLSVLSALECVDLVVLFEEDTPLRLIR"
BASE COUNT          587 a          765 c          766 g          503 t
ORIGIN      
        1 acagaagctg tttcatccaa ggtcaaattg tcaaagtgct tcaattcgac tgctacgagg
       61 gattgtagcc gaacgcacct tctttgagaa taggacttaa gactcatctt gcggaaagta
      121 cgtaaaaaag tctcgctttt cactttgaag accgcctctc cagaaaagcg gaaacgacat
      181 tccacacgtc cgaagaggag atcgttccca ggcactcctg cgtgcatcgc cagttgttgc
      241 atgctttcag aggacaccgc gtccaaaacg tccgttcagg gcgtgcaagg cccagtcctt
      301 cgggaagcgg cgtcggcccc agaagcgcga aggagggaac atccagcatt cgggcgatgt
      361 gaaggatacc ggtatcgccg gctacgacgg cgctggagag cgagatcgct cccccaaggt
      421 ccgcgagcgg aattttcccg accacgttgc ggaccagagg agaacacacg gcggtttcga
      481 tcacggacgc ggcggttttc tcctcctcgc cttcccccgc caaaacgacc tgaaggccgg
      541 cgcgcaccgc atacgcggtg aattccttcc aacgtcccac gggccatcgc ttgatctcct
      601 tgcttgcgcc gatagccccg agtatgaacg gacgggaggc atgctccccc agcagggagg
      661 ccgcccgctt ccgcatatcc ggcgtcacta cgtacccgag ggcgctcccc tcgggtatcc
      721 gaagatcgtg cgccgtccag aacgagagat cgcgcggcgt atcatagagc gcgccccatt
      781 tgctcgcgtt gcaaaaacgc agcggagtct tcgaaaagag agcgatgagc gccccccggt
      841 cgctggaatg gaaagaaaca agccgggtga agcgctctct tccgacgcgc cgcagaaggg
      901 agaaaaactc ccgctctccc gcagacgtgt cccaaggcag tacgctatcg atatacggct
      961 gttcccggag aaacggaaca tactgctcat acgccaacca tgttacatgg cttcgggggt
     1021 agctctcctt gagcgccctg caataaggcg taactatcag aatatcgccg agcgcactgt
     1081 atctcagaaa gagaatgcgt tccccttcgc cttctttcga agggggggtg taggtcctgc
     1141 aagaacggat catacacgga accaggaaca aaaaaacaaa tgaagaaccg ccacggaaca
     1201 gtcactcctt cgataacgcc acgtagaaag aaccggcggg gaaaatgcgc accgcttcgt
     1261 attctatcaa agatattgta aaatggagcc tgaaaaccga acgtgaattt gcccatcttt
     1321 cgatacttca aggagcgagt cgcaatgata ctccatagtg aaaagttcaa ggacattcgt
     1381 atcgccgtcg tgggagacgt tgttctcgat aagtacgtca gcggaatcgc gcggcgtata
     1441 tcctccgagg ccgccattcc ggtgcttctc gctcaaagaa taggtcatgt cgcgggcgga
     1501 gcggcgaacg tcgcggcgaa cctgtcgggg atcgggtgtt cggtctccgt gtacggcgct
     1561 gtcggcaacg acgggaacag tgtcctgctg ggcgcgatcc tctatgaaaa aaagatagag
     1621 aacaagctgc tgcagatcga cggccgcccc acgacgatca aaacccgcat cgttgccgga
     1681 aatcagcaga tcgcacgcta tgatatcgaa gagtcctcgg cgctctcgaa acacgacgaa
     1741 gacctgctct tttcatccct gctggaagaa atggacggtc tcgacgccat tatcctctcc
     1801 gattacggga acggcgtttt gaaaggttct ctctgctcaa gaatcatcgg gacggcggcg
     1861 acaaagggaa cgcccgtctt cgtcgacccc agggggaaca actggaaccg ctatcggggc
     1921 gccgcctgcg ccacgccgaa cgaggcggag ctggccttgg tctccgaggg cgcggagatc
     1981 gacgaacaga cgctctcgct tcatgcgcat tcggcacgga aacgctacgg gctccgaaat
     2041 ctgctggtga cgcgaggggc caaaggaatg gcactgttcc tcgaggacgg agaagagttc
     2101 ttccttcccg cgttttgcgt gcgcgaggtg ttcgacgttt ccggagcggg cgatacggtc
     2161 atcgcggttc ttgcggcatt gctgatcgcg ggggcgaact ggaagaaggc cgccgattgg
     2221 gcgaacaagg ccgccggcgt cgtggtcacg cgcgccggga cgtcccccgt ctcgctggag
     2281 gatttgcttt cgctcaatca agtggaaaga agcggcaagg tcgtctcctt cgaagaagcg
     2341 accgccagag tcgaggattg gaagcgagag gggaaaaccg tggtgtttac gaacggctgc
     2401 ttcgacctgt tgcactcggg acatgtccat ctgctccggc atgccgcgcg cgaaggcgat
     2461 cgccttgtcg tcggcgtcaa ttcggacgcg tccgtgcagc ggctcaaagg gccttcccgt
     2521 cccgtggcct cccaggaaga tcgcctctcc gttctttccg ctctggagtg cgtcgatctt
     2581 gtcgtccttt tcgaggagga cacgcccctc cgtcttatcc g
//
LOCUS       DAOPKU010000431         2612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_45252_length_2612_cov_3.560422, whole genome shotgun sequence.
ACCESSION   DAOPKU010000431 DAOPKU010000000
VERSION     DAOPKU010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45252_length_2612_cov_3.560422"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK849_11825"
     CDS             <1..201
                     /locus_tag="PK849_11825"
                     /inference="COORDINATES: protein motif:HMM:NF021921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPE66859.1"
                     /translation="NVKEGVIRQARFYGDYFGAENREEIERLLEGRFYREDALREALE
                     GFPLERSFKGAGTEEFLTFLMS"
     gene            complement(213..1658)
                     /locus_tag="PK849_11830"
     CDS             complement(213..1658)
                     /locus_tag="PK849_11830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE66860.1"
                     /translation="MFSMAAGPLSVFVFSALLLTCVFLLYERRRLRKIVNEEALAKSA
                     LGESIVRFHMIVESSAAATYVFRGGRFVMVNAAMEAITGYSREELLRMNSLEHIHPDH
                     RPSALERDAARERGEAVPTRYELKLLTKTGEVRWVDFTARTVLFDGVLCTVGSAFDIT
                     GRKRVEDALRESEAKYRFLTESMKDVIWTLDAETLRFVYVSSFVERLRGYTPEEIMAE
                     PLDAALTPESAALVHRLIDQETEKFKMREERGDAVFHTEEVEQPRKDGTTVWTEVVTV
                     YWRNPQNGRIEIHGVTRDISERKEVEAKMAHMAQHDPLTGLPNRALFSDRLERALAAS
                     RRSGIPTALMFLDLDKFKPVNDTYGHAIGDVLLQQAAERMKSALRESDTVGRIGGDEF
                     VVLLPAVERVEDALHVAEKLLEALRKPFFIEGRSVDVSCSIGIAIHPEDGRDEIELAK
                     NADTAMYWAKRDGGDCVRMFRTASDVSAAVL"
     gene            complement(1853..>2612)
                     /locus_tag="PK849_11835"
     CDS             complement(1853..>2612)
                     /locus_tag="PK849_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE66861.1"
                     /translation="IVQILKGVLLRKGVSFHTNSKVVGVEKKGKELAVRVETPAGPLV
                     LDAEKVLVSVGRRPVTKGLGLDRLGIAFDRARVKVNSRMETSVKGIYAIGDCASHIML
                     AHVASREGEVAAENIMGHAVDMDYKTVPSAVYTAPEIASVGLSESEAKGRGHRVKVGR
                     FPLMANGKSLIMNESDGMVKYVVDEKYDEILGVHIIGPRATDLIAQGGLALRLEATVD
                     EIITTIHAHPTVGEALLEGAMAVQGRAISLPKKA"
BASE COUNT          504 a          842 c          728 g          538 t
ORIGIN      
        1 aacgtgaagg agggcgtcat acggcaagcg cgattctacg gcgactactt cggcgcggag
       61 aacagggagg agatcgaaag gctgctcgaa gggcgcttct acagggaaga tgcgctccgc
      121 gaggcgctgg aagggttccc gctggagcgc tctttcaagg gagcgggcac ggaagagttc
      181 ctgacttttt tgatgtcctg agcatcgtat cgtcagagga ccgcagccga aacgtcggaa
      241 gcggtccgga acatgcggac gcagtcgcct ccgtccctct tcgcccagta catggccgtg
      301 tcggcgttct ttgcgagttc gatctcgtcg cggccgtcct ccggatgaat ggcaatgccg
      361 atgctgcacg agacgtcgac ggaacgccct tcgatgaaga atggtttgcg aagggcttcc
      421 aagagcttct ccgccacgtg gagcgcatcc tccacccgct ccaccgcagg gagcagcacg
      481 acgaactcgt cgccgccgat ccgccccacc gtgtcggatt cgcgcaacgc cgatttcatg
      541 cgttcggccg cctgttgaag cagcacatcg ccgatcgcgt gcccgtacgt atcgttcacc
      601 ggcttaaact tgtcgaggtc gaggaacatc agcgccgtcg ggattccgct tcttctggac
      661 gcggccagcg cacgctccag ccggtcggaa aagagcgccc ggttggggag accggtgagc
      721 gggtcgtgct gcgccatatg ggccatcttc gcctcgacct ccttacgctc ggagatatcg
      781 cgggtcacgc cgtgaatctc gattcgcccg ttctgcgggt tgcgccagta gacggtcacg
      841 acctctgtcc acacagtcgt tccgtccttg cggggctgtt cgacctcctc ggtatgaaac
      901 accgcgtctc cgcgttcttc ccgcattttg aacttctccg tttcttgatc gatgagcctg
      961 tggaccaagg ccgccgactc cggcgtcaga gcggcgtcga gaggttcggc catgatctct
     1021 tccggcgtat atcctcgaag ccgctcaacg aaggaactga catacacaaa ccggagcgtc
     1081 tccgcgtcga gcgtccagat gacgtccttc atgctctccg tcagaaatct gtatttcgcc
     1141 tcgctctccc tgagcgcatc ctccaccctc ttgcgtcccg taatgtcgaa ggcgcttcct
     1201 acggtgcaga gaacaccgtc gaagagaacg gttctcgcgg tgaaatcgac ccaccgcact
     1261 tcacctgtct tcgtgagcag cttcagttcg tagcgtgtgg gaacagcttc cccgcgctcc
     1321 cgggcagcgt cccgctccag cgccgaagga cgatgatcgg ggtggatgtg ttcgagagaa
     1381 ttcatcctca acagctcctc gcgggagtag ccggtgattg cctccatcgc ggcgttcacc
     1441 atgacaaaac gccctccgcg aaaaacatac gtcgcggcgg cggaactctc gacgatcatg
     1501 tggaaacgga cgatactctc tccgagagcg ctcttcgcga gcgcctcttc gttgacgatc
     1561 ttccgcagac gacgtctttc atacagcaaa aagacacagg taagaagcag cgcagaaaaa
     1621 acaaaaactg aaagaggacc ggcggccatt gagaacatat attttcctcc cgcgaaacca
     1681 gggaaaacgg cttcataatt tccaaacacc actaaggata gcatacctcg cttcctccgt
     1741 cgctcttttt ttctcatgtt ttctcatgaa cgaacttttc ccaggaaacg cagaaagcgg
     1801 cgttccggat caaccggtaa cgccgctttc atcagcagat tgttctggga tactacgcct
     1861 tcttgggcag cgatatggcc cgtccctgga ctgccatcgc cccctcaagc agcgcttcgc
     1921 cgaccgtcgg gtgcgcatgg atggtcgtga tgatctcgtc caccgtcgcc tccagccgga
     1981 gcgccagccc gccctgcgcg atgaggtcgg tggctcgcgg gccgatgatg tggacgccga
     2041 gaatctcgtc gtacttctcg tcgacgacgt acttcaccat gccgtcgctc tcgttcatga
     2101 tcaggctctt tccgttggcc atcagcggga agcggccgac cttgacgcga tgccctctgc
     2161 ccttcgcctc actctcgctc aagccgacgg aggctatctc gggagcggtg tacaccgcgc
     2221 tgggaacggt cttgtagtcc atgtcgaccg cgtgccccat gatattctcc gccgcgacct
     2281 ctccctctcg ggaggcgacg tgcgcgagca tgatatgcga cgcgcagtcc ccgatggcgt
     2341 aaattccctt cacggacgtc tccatccgtg agttcacctt cactctggcc ctgtcgaagg
     2401 caatcccgag gcggtcgagc cccaacccct tcgtaacggg acggcggccg acggagacga
     2461 gcaccttctc cgcgtcgagg acgagcgggc ccgcaggcgt ctccaccctc acggcaagct
     2521 ctttcccctt cttctccaca ccgacgacct tggagttggt atgaaaggaa acaccctttc
     2581 tcagaaggac ccccttgaga atctgaacta tc
//
LOCUS       DAOPKU010000432         2603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_45520_length_2603_cov_2.606750, whole genome shotgun sequence.
ACCESSION   DAOPKU010000432 DAOPKU010000000
VERSION     DAOPKU010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45520_length_2603_cov_2.606750"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /gene="buk"
                     /locus_tag="PK849_11840"
     CDS             complement(<1..1651)
                     /gene="buk"
                     /locus_tag="PK849_11840"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPE66862.1"
                     /translation="MSLHVLAINPGSTSTKIAWFTDGREIWRETVRHDPEALGVFPSV
                     AEQFQFRLDTIAEAVASKGCSFDDLSAVAGRGGLVEPIPGGTYAVDDVLLKRLRMGKP
                     WEHASNLGGILADAICRPRNIPAFIVDPVAVDEMNPAAKVTGLPELPKLSLGHALNVK
                     ATVRRAAKDLGEPWDSKNFIVAHIGGGITVCAHEKGRMTDLNNANDFGPFSPERAGGL
                     PAGDLLRFCFESGLTEKEVLRKVVGKGGLAAYTGTSDVREVKALIAEGDEYALRGTVA
                     GPISETVLGRLTAFTKSQKGHIKNVSYGRDLNGFDNWGLRGKILVQPSDALDLTFIAD
                     YRDTQQDCCAYQIRDTSTALNPVVGATLDSLLDPVVASPENRQTKLNAPVYNNSEQWG
                     LSGKAEYRFGNGYTFTSITAYREWDFENNLDVDALDFEDPIPGVVTFDLNSGQTSLSQ
                     TSQEFRLASPEWDHFDYVVGVYLFELDLDRSFRRRIEARTAAGIVGQSGQFNGSVGNK
                     NYAAFASGNYHFGQGTTIFGGLRLINETLEYQVYRDPANVLQ"
     gene            complement(1761..>2603)
                     /locus_tag="PK849_11845"
     CDS             complement(1761..>2603)
                     /locus_tag="PK849_11845"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPE66863.1"
                     /translation="LPAPLFEVERLWELKRLFGAPLLILPIYSERMVSGWLGLQLPES
                     PLLSSHESYLGMFCDLVGGVLLQQKRRSMTSERARILDGTSRGIVEILGRTLAMKDPY
                     TVGHQFEVARLARAMAERGGYDEAFLERVYYAGLVHDLGKIVIPSSILSKPGRLNTVE
                     FEIIKNHALHGWEILSSTEFPWPLADIVVQHHERLDGSGYPHGLKGAEIRTEARFIAV
                     ADVVEAMTSHRPYRPGLGIERALEEIRTHSGTWFDPLVVELCLAVIREGFVFDDKSYK
                     VALP"
BASE COUNT          491 a          813 c          797 g          502 t
ORIGIN      
        1 gctgaagaac gttcgccggg tcgcggtaga cctggtattc caacgtctcg ttgatcaggc
       61 ggaggccgcc gaagatcgtc gtgccctggc cgaagtgata gttcccgctg gcgaaggcgg
      121 cgtagttctt gttgccgacc gagccgttga actggccgct ctggccgaca atgcctgcag
      181 ctgttctcgc ctcgatgcgg cggcggaatg agcggtctag gtccagctcg aacaggtaga
      241 cgccgaccac gtaatcgaaa tgatcccatt cgggggaggc gaggcggaat tcctgcgagg
      301 tctgcgagag gctcgtctgg ccgctgttca ggtcgaatgt gacgacgccc ggaatcgggt
      361 cctcgaagtc gagcgcgtcg acgtcgagat tgttctcgaa atcccattcc cggtacgcgg
      421 tgatcgaggt gaaggtgtag ccattgccga agcggtattc tgccttgccg gagaggcccc
      481 attgctcgga attgttgtag accggcgcgt tcagcttggt ctggcgattt tccgggctcg
      541 cgacgaccgg gtcgagcagc gagtccagcg tggcgccgac caccgggttc agggcggttg
      601 aggtgtcgcg gatctggtag gcacagcagt cctgctgcgt gtcgcggtaa tcggcaatga
      661 aggtcaggtc gagcgcgtcg ctcggctgga ccaggatctt tccgcgcaga ccccaattgt
      721 cgaagccgtt caggtctcgt ccgtaggaga cgttcttgat gtgccccttc tgggacttcg
      781 tgaatgccgt gaggcggccg agcaccgttt cggagatcgg gcctgcgacc gtaccgcgaa
      841 gggcgtactc gtctccttcg gcgatcagcg ccttcacctc gcgcacgtcg ctcgtgcctg
      901 tgtatgcggc gaggccgccc ttccccacga ctttgcggag aacctccttc tccgtgaggc
      961 cgctctcgaa gcagaatcgg agcaggtccc cggcaggcag gcctccggct ctctcgggag
     1021 agaatgggcc gaagtcgttg gcgttgttca ggtcggtcat ccgccccttc tcatgggcgc
     1081 agacggtaat gccgccgccg atatgggcga caatgaagtt cttggaatcc cacggctcgc
     1141 cgagatcctt ggcggcacgg cgcactgtgg ccttcacgtt gagcgcgtgc cccagcgaaa
     1201 gcttgggaag ctcgggaaga ccggtgacct tcgccgcagg gttcatctcg tcgacggcga
     1261 ccggatcgac aataaacgcc gggatattcc tcggcctgca gatcgcgtcg gcgaggatgc
     1321 cgccgaggtt cgaggcgtgc tcccacggct tccccatccg gagacgcttc aggaggacgt
     1381 cgtccacggc gtacgtgccg ccgggaatcg gttcgacaag accgccgcgc cctgcaaccg
     1441 cggagaggtc gtcgaaggaa caccctttcg acgctaccgc ctcggcgatg gtatccaggc
     1501 gaaactgaaa ctgctccgct actgacggga agacgcccaa cgcctcggga tcatgccgga
     1561 ccgtctctct ccatatctcc cgcccgtcgg tgaaccaggc tattttcgtg cttgtggagc
     1621 cgggattgat tgcaagaaca tgcaacgaca tacagaccct ccccttcgga accgcgatca
     1681 tctcttctgt cctctgtttt cagattcaaa agctattaac agaactcatt atacagtata
     1741 ttgttctccc ggacaaaggc tcacgggaga gcgactttgt aacttttgtc gtcgaagaca
     1801 aagccctccc ggatcacagc aagacacaac tccaccacca aggggtcgaa ccacgttccg
     1861 ctgtgcgtgc ggatctcctc cagggctctc tcgatcccca gtccaggacg gtacggcctg
     1921 tgggaggtca tcgcctccac cacgtccgcc acggcgatga aacgcgcttc ggtgcgaatc
     1981 tccgcccctt tgaggccgtg cgggtacccc gagccgtcga ggcgttcatg gtgctggacc
     2041 acgatatcgg cgagcggcca cggaaactcc gtcgaagaga gaatctccca tccgtgcagc
     2101 gcgtggttct tgatgatctc gaactccacc gtgttcaacc gccctggttt tgagaggatg
     2161 gacgacggaa tcacgatctt gcccaggtcg tggacaagac cggcgtaata cacccgttcc
     2221 agaaaggcct cgtcgtaccc tccccgctcc gccatcgccc gggcaaggcg cgccacctcg
     2281 aactggtgcc ccacggtgta cggatccttc atggcgagcg tccgcccaag aatctcgacg
     2341 attccgcgcg aagtgccgtc gaggatgcgc gcgcgctcag aagtcatact ccgccgcttc
     2401 tgctgcagca ggaccccccc gacgaggtcg cagaacattc caaggtacga ttcgtgcgaa
     2461 gaaagaagag gactctccgg aagctgaagc ccgagccaac cgctcaccat ccgttcagag
     2521 taaatcggaa gtatcaggag cggggctccg aagagccgtt tcagttccca aaggcgctcc
     2581 acctcgaaga gcggcgccgg cag
//
LOCUS       DAOPKU010000433         2602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_45535_length_2602_cov_4.060856, whole genome shotgun sequence.
ACCESSION   DAOPKU010000433 DAOPKU010000000
VERSION     DAOPKU010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45535_length_2602_cov_4.060856"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2602)
                     /locus_tag="PK849_11850"
     CDS             complement(<1..>2602)
                     /locus_tag="PK849_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66864.1"
                     /translation="ETNLLDAWLEMTFTLPWNRGTYEPHDLDIVMNGQVIGTVKNAIP
                     EGNYRFPVPASAIRFGASGEPEGNGVEIVSRHLRGGHYVVNSDFRLVTRLNAARAWTV
                     AESREEAEAAARSMEGVIADRAKISISSQGISLPEGGSLKAGAETTLTATLRNLGAMP
                     GYNVGVVLSIADPNGTERELRRSYIPFVPVTGEIPVRFTFSLPPGKHRLKLSLNPDNR
                     PENSAYIWAEVGGDDQRPTLDLLEPADGAKLDGTILKIKAHAMDDVRVARVEAKIDQG
                     LWSRLQREADGMFTAKGVIQPGERVITVRALDGSGNVTETTAKVVVEAPLPDGEILEP
                     VSGSTLDERHTMLKVRCEANVAAAAYRVNQGPWTPMRVTQGTAEERIPLRFGKNDIEV
                     RLLDAKRAERALSADVTSTGQRAAEEDTPEKDKKDAPEIPPEAPEAPEAHEEQEPGDE
                     TPPQMPEIPGGDEEVDSLLDQIQELMGMPPEGGTPPDHEGPEGAEDGPLPGGDDQEPE
                     TAQDPGTIDIPDVGTVDVTGPSDFLLPAEEDEDDDSWLDAGGDLEEEEPLEPEEIEIE
                     EEGGEDVGDEPDDEEITGEEFFTETDLFDGFDGLDEETEPLDLEAWVEPKSNPDAPEQ
                     DPMYECPLEPFDAADTDPSSPFTPPSGLQSDPGGCVAVETVQKDWYCTNRPNINVKFQ
                     LPDWLKKLDLPKPGTKEYDEMVKKLLQHLRDRGIDTAAFERFQDALIRRAGRLEHPDE
                     LPTFLQSLGLTSMPKPSPDDVEANKRWREAMENGARAWYLRLLASGDPQMIYQGLKAR
                     GEALGKFDEALGLAADAMMTEIKANQQLTEQALWSLPYVGPAMDVLSIITGETLSGEA
                     MTPERAAMMLG"
BASE COUNT          348 a          908 c          800 g          546 t
ORIGIN      
        1 tccgagcatc atcgccgcgc gctccggcgt catggcctct ccggagagcg tctcgcccgt
       61 gatgatcgaa aggacgtcca tcgccgggcc gacgtagggc agcgaccaga gcgcctgctc
      121 cgtcagctgc tgattcgcct tgatctccgt catcatggcg tcggcggcca ggccgagcgc
      181 ttcgtcgaac ttgcccaacg cttcaccgcg cgccttcagg ccctggtaga tcatctgcgg
      241 atcgccggag gccagcaggc ggagatacca tgcgcgggcg ccgttctcca tggcttcgcg
      301 ccagcgcttg ttcgcctcga cgtcgtccgg cgacggcttg ggcatggagg tcagcccgag
      361 cgactgcagg aacgtcggca gctcgtcggg gtgctccagc ctccccgccc tccggatcag
      421 ggcgtcctgg aaacgttcaa acgccgcggt gtcgatgccc ctgtcgcgca gatgctgcag
      481 aagcttcttc accatttcgt cgtactcttt cgtccccggc ttgggcaggt cgagcttctt
      541 cagccagtcg gggagctgga acttcacatt gatgttcggc ctgttggtgc agtaccagtc
      601 tttctggacg gtttccacgg ccacgcagcc gcccggatcg ctttgcagcc ccgacggtgg
      661 cgtgaagggc gagctcgggt cggtgtcggc ggcgtcgaac ggttccaggg ggcactcgta
      721 catcgggtcc tgctcgggag cgtccggatt gctcttcggc tccacccacg cttcgagatc
      781 cagaggctcc gtttcttcgt ccagcccgtc gaatccgtcg aagaggtcgg tttcggtgaa
      841 gaactcctct ccggtaatct cctcgtcgtc gggttcgtcg ccgacgtcct cgccgccctc
      901 ttcctcgatc tcgatctcct cgggttccag cggctcttcc tcttcaagat cgccgcccgc
      961 gtcgagccac gagtcgtcgt cctcgtcctc ctcggcaggc agcaggaagt cggagggccc
     1021 cgtcacatcg acggttccca catcgggaat gtcgatcgtc ccgggatcct gcgccgtctc
     1081 cggctcctga tcatcccctc cgggcagagg accgtcttcc gcaccctccg gcccctcatg
     1141 atccggaggc gtcccgccct cggggggcat tcccatgagc tcttgtatct ggtcgagaag
     1201 ggagtcgacc tcttcgtccc cgccgggaat ctccggcatt tggggaggcg tttcatctcc
     1261 tggctcttgc tcctcatgcg cttcaggcgc ttcaggcgct tccggaggta tctccggcgc
     1321 atcctttttg tccttttctg gggtgtcttc ctccgctgcg cgctgcccgg tggaggtcac
     1381 gtccgcggag agcgcccgtt ccgctctctt cgcgtcgagc agcctgactt cgatgtcgtt
     1441 tttcccgaag cgcagcggaa tacgctcctc cgccgtcccc tgcgtcacgc gcatcggcgt
     1501 ccacggcccc tgattcactc tgtacgccgc cgcggccacg tttgcttcgc agcgcacctt
     1561 cagcatcgtg tgccgctcgt cgagagtgct gccggatacc ggctccagaa tttcgccgtc
     1621 gggaagcggc gcctcgacga cgaccttcgc cgtcgtctcc gtgacgttgc cgctgccgtc
     1681 gagggcgcgg accgtgatca cgcgctcgcc cggctggatc actccctttg ccgtgaacat
     1741 gccgtccgct tcgcgctgaa gccgcgacca gagcccctgg tcgatttttg cctcgacgcg
     1801 cgccacgcgg acgtcgtcca tcgcgtgcgc cttgatcttc agaatcgttc cgtcgagctt
     1861 cgcgccgtcg gctggctcca gcagatcgag cgtcggccgt tggtcgtcac cgccaacctc
     1921 cgcccatatg tacgccgagt tctcgggccg gttgtccggg ttcagcgaga gcttcaggcg
     1981 gtgtttgccc ggaggtagtg agaaggtaaa gcgaaccggt atttctcccg tcaccggaac
     2041 gaaggggatg tagcttctcc gcagctctcg ctccgtcccg ttcggatcgg cgatgctcag
     2101 aacgacgccg acgttgtacc ccggcatcgc gccaaggttc cgcagcgtcg cggtcagcgt
     2161 ggtttcggcc ccggctttga gcgagccgcc ctcgggaagc gagattccct gggaggagat
     2221 gctgatcttc gccctgtcgg cgatcacccc ctccatggag cgggccgccg cctccgcttc
     2281 ctcgcgcgac tccgcgacgg tccatgcgcg cgccgcgttg aggcgcgtca ccagccggaa
     2341 gtcgctgttc acgacgtagt ggccgcccct caggtggcgc gagacgatct cgacgccgtt
     2401 cccttcaggc tcgccgctcg ctccgaaacg gatcgccgac gcgggaaccg ggaagcggta
     2461 gttcccctcg ggaatcgcgt tcttgactgt gccgatcacc tgaccgttca tcacgatgtc
     2521 gaggtcgtgc ggctcgtagg ttccgcggtt ccatggaagg gtgaaggtca tctcgagcca
     2581 ggcgtccagc aggttggtct cc
//
LOCUS       DAOPKU010000434         2589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_45874_length_2589_cov_3.459747, whole genome shotgun sequence.
ACCESSION   DAOPKU010000434 DAOPKU010000000
VERSION     DAOPKU010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45874_length_2589_cov_3.459747"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..818
                     /locus_tag="PK849_11855"
     CDS             297..818
                     /locus_tag="PK849_11855"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HPE66865.1"
                     /translation="MGKYAVRESKSKFYFNLKADNGEIILTSEGYNREASCLQGVESV
                     RINAPVAAVEDQTVEGFKKETNPKFEVYTDKAGEFRFRLKARNGQIIGVSEGYKAKAS
                     AIKGIESIRKNSSSDVMKVENAVKEEKAEAPVKAEKAAKEEKPAKAEKPVKAEKPAKV
                     EKAAKAPAKKKKA"
     gene            1179..>2589
                     /locus_tag="PK849_11860"
     CDS             1179..>2589
                     /locus_tag="PK849_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HPE66866.1"
                     /translation="MKYLREVRDSIKFFSVELELFAPVSAIRRGMMSVIPLVMAGSLA
                     LALRSLPIAPYQSFLKQAFGGALERLLGFVQNGTLDILSLLLTFAIAHAYALKKNESL
                     KEKLSPLIVSITALSSFIIFRCAYSGESLITAFGNTTVAGGVVSALLASMLFVRLRRI
                     QLFRIGVYSADAYAFYNSSLSSIFPAMTTIALFALSAVLYKEFLAPANLTGGWAGDMY
                     AALLEKGPVLGSALLFVFFVHLMWCFGIHGNNVLEAVAQDLFVPALQVNQELVASGQL
                     PTEIVTKTFLDTFVLMGGSGATLCLVAGILLFSKSRSERRLIKLSSLPLLLNVNELII
                     FGIPIVFNPVYFIPFTLLPVLLTATSFFAVSLGLVPHTVNHVEWTTPVFVSGYIATGS
                     FRGVALQLFNIVLGTLCYLPFIRLARLATDKKISNALDKAYDMLENDPLRFTMHGQTE
                     TADFLRFLALDLENDLQTEK"
BASE COUNT          576 a          697 c          684 g          632 t
ORIGIN      
        1 cacagattct ctttgtcatt gttccaccag cctttccaat gataggggca gggcccacct
       61 tgctatatcg cgattattct atcatgacac cctcgcacgg cggaatggtg ggggggcgct
      121 ttccagggtg gtttttctcg ccctttgcag ctctgttccg ttctcgattg tgaaaaccgc
      181 cgacagaaga agcgcgaatc ggcgtttttt cgcatattag ctgaaaacta tggttgaccc
      241 ggctatttat acgttctaca ataaacaaaa ttagcaaatc attgggaggg gatcgtatgg
      301 gtaagtatgc tgttcgcgag tcaaagtcca agttctattt caatctgaaa gcggacaacg
      361 gtgagatcat cctgacctcc gaggggtaca accgggaagc cagctgcctt cagggtgtcg
      421 aaagcgttcg cataaacgcc cccgtggctg ctgtcgagga ccagactgtc gaaggcttca
      481 agaaagagac caatcccaag tttgaagtgt acaccgataa agccggagag ttccgtttcc
      541 gtctgaaagc caggaacggc cagatcatcg gcgtctccga agggtacaaa gcaaaagcga
      601 gcgcgatcaa aggaatcgag agcatcagga aaaactcctc ttccgacgtc atgaaagtcg
      661 agaatgccgt aaaagaggaa aaagccgagg cgccggtaaa agctgaaaaa gctgcgaaag
      721 aggaaaaacc ggcaaaggcg gaaaaaccgg tgaaggctga aaaacccgcg aaggtcgaaa
      781 aagccgcaaa agcaccggcg aagaagaaaa aggcgtaacc tgtcgccgtt gacggcggga
      841 acccataccg tgggctcggc gcgcggcgat gttctctcaa attgtcctag cactgcttcc
      901 ttttcgcagg agggtggagg acgggagctt cccgttcttc actctcctgt gttgtctggt
      961 cattcccgtg agttgttttt actaaagtgc aactggtctt gtttctagta agttttttat
     1021 tctattaaaa acaggttgct ctttagcaag aggctttgta taatgcggta tatactcgaa
     1081 aattttccag tagctttctc aaaaaagaca cttggcgccg tacataagcg caagattgcg
     1141 ggcagctcta taagccgggg agaaaaggag ccggggcaat gaaatatcta cgggaggttc
     1201 gcgactccat aaagttcttc tcggtggaac ttgagctttt tgctcctgtc tccgctatac
     1261 ggcgcgggat gatgagcgtc attcccctgg tgatggccgg ttctctggcc ctcgctctcc
     1321 gcagtctccc catcgctccg tatcaaagct ttttgaaaca ggctttcggc ggcgccttgg
     1381 aacgcctgct cggcttcgtg cagaacggaa ctctggatat tctctctctg ctgctgacct
     1441 tcgctattgc tcatgcctac gccttgaaaa agaacgagtc tctcaaagag aaactcagcc
     1501 cgctcatagt ttccataacc gcattgagct cgtttatcat ttttcgctgc gcgtactcgg
     1561 gcgaatcgtt gatcactgcc ttcggcaaca caacggtcgc agggggagtc gtttcagccc
     1621 tgctcgcgtc aatgctcttc gtccggttga ggagaataca actcttcaga atcggagttt
     1681 actctgcgga tgcctacgcg ttttacaact cgtcgctgtc gtccatcttc cctgccatga
     1741 cgacgatcgc ccttttcgcc ctttccgccg ttctgtacaa agagtttctg gcgccggcaa
     1801 acctgaccgg gggatgggcc ggagacatgt acgctgcgct gctcgaaaag ggtccggttc
     1861 tgggaagcgc gctcctcttt gtcttttttg tccacttgat gtggtgtttc ggcatccacg
     1921 gcaacaacgt gctcgaagcg gtcgcgcagg atcttttcgt tccggcgctt caggtaaatc
     1981 aggagctcgt cgcctcaggg cagcttccca cggagattgt gaccaagaca tttctggaca
     2041 cattcgtcct gatgggagga agcggcgcga cgctctgcct tgtggcggga attctgctct
     2101 tcagcaaaag ccgctcggag cgccgcctga tcaagctctc ctcgctgccc cttctgctca
     2161 acgtcaacga gctgatcatt ttcggaattc cgatcgtctt caatcccgtc tacttcatcc
     2221 ctttcacgct cctcccggtt ctgctgacgg cgacgtcgtt tttcgcggtc tcgctcgggc
     2281 tggttccgca caccgtcaat catgtggagt ggacgacgcc cgtcttcgtc agcgggtaca
     2341 tcgcaacggg gtcgtttcgc ggcgttgcgc tgcaactctt caacatcgtc ctgggaacgc
     2401 tctgctatct gccgttcata cgtctggcgc ggttggccac cgataaaaaa atctcgaatg
     2461 cgctggacaa agcctacgat atgcttgaga acgatccgtt gcgctttacg atgcacgggc
     2521 agacggaaac ggcggatttt ctccgtttcc tggcgctgga ccttgagaac gacctccaga
     2581 ccgaaaagc
//
LOCUS       DAOPKU010000435         2586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_45942_length_2586_cov_3.706835, whole genome shotgun sequence.
ACCESSION   DAOPKU010000435 DAOPKU010000000
VERSION     DAOPKU010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45942_length_2586_cov_3.706835"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PK849_11865"
     CDS             <1..865
                     /locus_tag="PK849_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPE66867.1"
                     /translation="DVKWGTPSPVQLQDPKYGVLLPVRSYGQFGVRIEDSRKFLVKLV
                     GARDQFDREAPAHHFRGLLLTRIQDLISTYLVHRGISVVEVNAWLDEISEEIHGKIAG
                     LFGDYGIEVLNFFVNSINVPEDDPAVAELKKTLSERAKMEVLGYNYQQMRSFDTLEKA
                     ASNEGGGNLMNAGLGLGVGMGVGGAFGRLAGEMGSNLTTGGGEPVPSVRCAKCGASAG
                     RGAKFCSECGASLPGRVCPSCGKPLPEGAKFCAECGASLVPRCPSCGKEASPGARFCA
                     ECGSAIGRKEE"
     gene            905..1672
                     /locus_tag="PK849_11870"
     CDS             905..1672
                     /locus_tag="PK849_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66868.1"
                     /translation="MFITPERTFSKSFILLVIMSVILAGLSAVIFLLVPPGARTEGFW
                     ISFWAILFSVVLSFSYMIFHVVAGKDDFAPVPLLLGLSTTIACYCIFVLGNVLVSHFW
                     LRLPQNAYIATHIAGFMLLVGGGGAITLLSLTAKETDVSASLKRSRLFVQTTRVASLV
                     DELSLSPFAGQAAEMIARLRKLKEAIQYSDPMAPDGLEAEELVVIAVGAVEEKARRFL
                     SAATDQERRAFLDEAAPLVEKAFNTLHRRNEEVKESK"
     gene            1886..>2586
                     /locus_tag="PK849_11875"
     CDS             1886..>2586
                     /locus_tag="PK849_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPE66869.1"
                     /translation="MERLEKVILENLRSRPQEECLWWENGWMTSLDLLSFVEKNVQVL
                     KKAGFKKGQRIAVLLPNSPQVLALSLAAWSLGGAISPLNAKSGLPSLMGTLNLVEPCA
                     VVLAPGLDELKEALREQQVPVVLAESLSTPLPSFSVRETSVEDESLAVIFATSGTTGL
                     PKAVPLTHGNLLDNALRMYDSLELLAEGDNLLNVLPNFHSFGYTVAGLMPLVRNLRQT
                     LLASFLPPVEAMKTIR"
BASE COUNT          502 a          714 c          787 g          583 t
ORIGIN      
        1 tgacgtgaag tggggcacgc cctctcctgt gcagcttcag gatcccaaat acggcgtcct
       61 gctcccggtc aggtcgtacg gacagttcgg cgtccgcatc gaagactcgc gcaagttcct
      121 cgtaaaactg gtgggggcgc gcgatcagtt cgaccgagag gcgccggctc atcacttccg
      181 ggggttgctg ctcacgcgga tacaggatct tatctcgacg tatcttgtgc atcgcggtat
      241 ctccgttgtt gaggtgaacg cctggcttga cgaaatctcc gaggagattc atgggaagat
      301 cgcggggctg ttcggggatt acggaatcga agtgctcaat ttcttcgtga actcgatcaa
      361 cgtccctgag gacgatcctg cggtggctga gctgaaaaaa acgctctccg aacgggcaaa
      421 aatggaggtt ctggggtaca attaccagca gatgcgctcg ttcgataccc ttgaaaaagc
      481 tgcgtcgaac gaggggggcg gcaatctgat gaacgccggt ctgggacttg gagtggggat
      541 gggagtcggc ggagcgttcg gcagactggc cggcgaaatg gggagcaatc tgaccaccgg
      601 agggggagaa ccggttccgt cggtccgatg cgcgaagtgc ggcgcttctg caggccgggg
      661 ggcgaagttc tgctcggagt gcggcgcttc tcttcccgga agagtctgtc cgtcatgcgg
      721 caaaccgctg ccggaggggg cgaagttttg tgcggagtgc ggcgcttcgc tggttccgag
      781 atgcccttcc tgcgggaagg aggcgtctcc cggcgccaga ttctgcgccg agtgcggttc
      841 cgccatcggc cggaaagaag agtaacaggt attcggcatt tcaagaaggg agtagttagc
      901 tactatgttc attacaccag agaggacgtt cagtaaaagt tttattttgc ttgtcataat
      961 gtcggtgatc ctcgcggggc tctcggcggt catttttctg cttgtgcctc ccggagcgcg
     1021 aacggaaggt ttttggattt ccttctgggc gatcctcttt tcggttgtcc tctcgttctc
     1081 ctacatgatc ttccacgtcg tcgcggggaa agacgacttt gcgccggttc cgctgctgct
     1141 ggggctttcc acgaccattg cctgctactg tattttcgtc ctgggcaacg tgctcgtttc
     1201 acacttttgg cttcgcctgc cgcagaacgc ctatatcgcc acgcacatcg ccggtttcat
     1261 gctgctggtg ggaggaggag gcgccatcac ccttctctcg ctcacggcaa aggagaccga
     1321 cgtgagcgcg tcgctcaagc ggagccgtct tttcgtacag acgacgcgag ttgcctccct
     1381 cgtcgacgaa ctcagcctct ccccgttcgc cggacaggcc gccgaaatga tcgcccgctt
     1441 gcggaagctc aaggaggcga tccagtacag cgatccgatg gctccggatg gattggaagc
     1501 cgaagaactg gtcgtcatcg ccgtgggagc agttgaagaa aaggcccgcc gttttttgtc
     1561 ggcggctacc gatcaggagc gccgcgcctt cctcgacgaa gccgcgcccc ttgtggagaa
     1621 agctttcaac accctgcaca ggcggaacga ggaggtcaag gagagcaaat agtcgtttcg
     1681 cacacatcga gatttccgga aagaacccgg gatagaagcg tatattttca caacgtagcg
     1741 tctttttgtg gagccctttc tgtttttttt atgcagaaag ggcttttttg ctttacaata
     1801 gagaggtcac cctacggtga tataccttcc gtcattactg ctgtgaactg ccacattcat
     1861 atactccgaa aggggagaag aacagatgga gaggctggaa aaagtcatac tggagaattt
     1921 gcggagccgt ccgcaggaag agtgtctctg gtgggagaac gggtggatga cctccttgga
     1981 tctgctcagt tttgtggaga agaatgttca agtcttgaaa aaggcaggat tcaaaaaagg
     2041 gcagaggatc gcggtcctgc tgccgaactc cccccaggtg ctggcgctct ccctggctgc
     2101 ctggagcctg ggcggcgcca tatcgccgct caacgcgaag tccggactcc cctcgttgat
     2161 gggaacgctg aacttggtcg agccgtgcgc cgttgtcctc gcccccggac ttgacgaact
     2221 caaagaagcg cttcgggagc agcaagtacc cgtcgttctt gccgagtctc tctctacgcc
     2281 gcttccatca ttctccgtcc gtgagacgag cgtcgaggac gagtcgctcg cggtgatttt
     2341 cgccacgtcc gggacgaccg gtctcccaaa ggccgttccg ttgacccacg gcaacctttt
     2401 ggacaacgcg ctgcggatgt acgattccct cgaactcctg gccgaggggg ataatctgct
     2461 caacgtgctg ccgaacttcc attccttcgg ctacaccgtg gccgggctta tgccgctggt
     2521 ccgcaacctg cgtcaaacgc ttcttgcgtc gttcctgccg cctgtggagg caatgaagac
     2581 gatccg
//
LOCUS       DAOPKU010000436         2558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_46634_length_2558_cov_4.082701, whole genome shotgun sequence.
ACCESSION   DAOPKU010000436 DAOPKU010000000
VERSION     DAOPKU010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46634_length_2558_cov_4.082701"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..312)
                     /locus_tag="PK849_11880"
     CDS             complement(127..312)
                     /locus_tag="PK849_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66870.1"
                     /translation="MEELLRVSNEIIHQIYFVLAGLCGLVLLRGLFSRNTRKTIVYDI
                     VYAYTIIPFLLRALHIK"
     gene            complement(333..1628)
                     /locus_tag="PK849_11885"
     CDS             complement(333..1628)
                     /locus_tag="PK849_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HPE66871.1"
                     /translation="MYAETSFVLAVMVFTFALASWKLKSPELSMVITAVVGALAGGLG
                     FPVRLLVEGTFTYFDVAFTFLTASIFINFYSATGAMDALVRKMVDRFFDRKWILFFLL
                     AVIMLIPGALTGAGSVSMFVVGGMVATVLRFSGIKETRIVAFIYVTSMLAAVAPPINL
                     WVMLMCAQANMPYVGFEFPLLAPIALITVFTVFYLLRGGSPKDKETVLASLPEAHERM
                     NWFRIAAPLLVFVVLILLSKYAAFSTPVLGIPLIFIISTAVAMLVSPVRKGALHWYGV
                     LANTSEQVFPLLATVISVGVLVNIMTATGVRGLIAITFVTLPVYLIYAFALVVLPFAQ
                     GSLSYSSGIILGTPLIFLFNSVGVNVTIVATALSLIFPLGDCLPPSRISGRVAIEVSG
                     YQGTYVSFLRAIFVPALFMGLVALGMLMYANHFRWLIVY"
     gene            complement(1692..2276)
                     /locus_tag="PK849_11890"
     CDS             complement(1692..2276)
                     /locus_tag="PK849_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPE66872.1"
                     /translation="MRKIAAYSLLVCLLFASVSFAAVTGAEAPKGEEPLLITNAGQGP
                     GGKMGRLLVARAEVVEQLTYNAEPKPEDIAAGGYKTILVVIGSSAKGLGASGITIDQE
                     VDRLHAIMEKAKELNIQVIAAHIEGKARRGKPGSADERSIDAILPYAAHIVVNKDGDL
                     DKKFTRFGEEHSIPVSYLDNAMDLNAFAKTMYAK"
BASE COUNT          584 a          707 c          788 g          479 t
ORIGIN      
        1 tctccttcat cgtgcgcagc tcggtgaact ccctgccgcc cagccaggca agtgccagcg
       61 cgagcagcag caagacgact ttccggggcg ttttgaattt ttcgtagtcg aacattcgcc
      121 gtcaccctac tttatgtgca gcgcgcgcaa caggaaggga atgatcgtgt aggcgtagac
      181 gatgtcgtac acgatggtct tgcgcgtgtt gcgggagaac agcccccgaa ggagaacgag
      241 gccgcacagt ccggccagga cgaaatagat ctgatggatg atctcgttgg atacgcgaag
      301 aagctcttcc ataatccaga ctcctctctt ttctaataca cgatgagcca tcggaagtga
      361 ttggcgtaca tcagcattcc gagcgcgacg agccccatga agagcgcggg gacgaagatc
      421 gccctgagga acgagacgta cgtcccctgg taccccgata cttctatggc gaccctgccg
      481 gagatcctcg acggcggcag acagtcgccc agcgggaaaa tgaggctgag ggccgtggcc
      541 acgatggtca cgttgacgcc gacggagttg aagaggaaga tcagcggcgt tcccaggatg
      601 attccgctgc tgtagctgag cgacccctgc gcgaaaggaa gcaccacgag agcgaaggcg
      661 taaatcaaat acaccggcag cgtgacgaag gtgatggcga tgagcccccg gacgccggtc
      721 gcagtcatta tgttcaccag gacgccgacg ctgatgacgg tcgcgagcag cggaaagacc
      781 tgctccgatg tgttggcgag cacgccgtac cagtggagcg cccccttccg cacgggtgag
      841 acgagcatgg cgacggcagt gctgatgatg aagatgagcg ggattccgag caccggcgtc
      901 gagaaggccg cgtacttgga gagcagaatc agcaccacga agaccagcag cggcgcggcg
      961 atacggaacc agttcatccg ttcgtgcgct tcgggaaggg aggcgagcac cgtctccttg
     1021 tccttggggg agcctcctcg cagcaggtag aagactgtga agaccgtgat gagcgcgatg
     1081 ggggccagaa gagggaactc gaaccccacg tagggcatgt tggcctgcgc gcacatgagc
     1141 atgacccaga ggttgatcgg cggcgcgacg gcggccagca tcgacgtgac gtagatgaag
     1201 gcgacgatgc gcgtctcctt gatgccgctg aagcggagca ccgtggcgac cattccgccg
     1261 acgacgaaca tggagacgct ccccgcgccg gtcagcgcgc cgggaatgag cataatgacg
     1321 gcgagcagaa agaaaagaat ccacttgcgg tcgaagaacc ggtccaccat cttgcgcacc
     1381 agcgcgtcca tcgcgccggt ggccgagtag aagttgatga agatggaggc cgtcagaaag
     1441 gtgaaggcca cgtcgaagta ggtgaacgtc ccttcgacca ggaggcggac cgggaatccc
     1501 agccctccgg cgagcgcgcc gacgacggcg gtgatcacca tcgacagttc gggtgatttc
     1561 agcttccagc tcgcgagggc gaaggtgaac accatcacgg ccagaacaaa cgatgtttcc
     1621 gcgtacatct caggacctcc cgtccgttga aaagaaaaga ccccgtcgaa agatacggcg
     1681 gggtccggtt tttattttgc gtacattgtt tttgcgaatg cgttgaggtc catcgcgttg
     1741 tcgaggtagc ttacgggaat tgagtgctct tcaccgaagc gggtgaactt tttgtccaga
     1801 tcgccgtcct tgttcaccac gatatgcgcg gcgtacggca gaatggcgtc gatggagcgt
     1861 tcgtcggcgc tccccggttt gccgcggcgg gccttgccct cgatatgcgc ggcgatgacc
     1921 tggatgttca gctccttggc cttctccatg atcgcgtgca gacggtcgac ctcctgatcg
     1981 atcgtgatgc ccgaagcgcc gagccccttt gcggaggagc cgatgacgac gaggatggtc
     2041 ttgtagccgc cggccgcgat gtcctcgggc ttcggttctg cgttgtaggt caactgctcc
     2101 acgacttccg cgcgggcgac cagcagacgg cccatcttgc ctccggggcc ctgacccgcg
     2161 ttggtgatca gcaggggttc ttcccccttg ggcgcctcgg ctccggtaac agccgcgaac
     2221 gagacggacg cgaacagcag gcagacgagc agagagtacg ctgcgatttt tctcataaca
     2281 aagaaacctc ctttggaatt atgataaaga aacataccga atacatatcg gtaaacgata
     2341 cgtggtgtta cttcttgaaa taaaaatgta cttccttgca ttttcggatg cgatgcacgg
     2401 cgtagaatgt tgtatatatc aataacgtca atgatatacc tccctttgaa cgatgtcaac
     2461 atagaccgga caatttcgga agaatcagcg gttgcgtgaa tacaatatac atgagcagcg
     2521 agtcgctcaa ataaaacgcc ggactcttca aaaaagag
//
LOCUS       DAOPKU010000437         2552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_46783_length_2552_cov_4.277533, whole genome shotgun sequence.
ACCESSION   DAOPKU010000437 DAOPKU010000000
VERSION     DAOPKU010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46783_length_2552_cov_4.277533"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..682)
                     /locus_tag="PK849_11895"
     CDS             complement(218..682)
                     /locus_tag="PK849_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPE66873.1"
                     /translation="MIELEKRLSDDLVAAMKSRKEMELSSLRMLKAEVQRAKAEKGRS
                     VELTEDDIVALIQRLVKQRREAADQYTLVGAGDRAAEELREALFLETYLPEQLSDEEL
                     EHIVEEAGAFVKAAGPKDMGKVMGKVMAAVKGRADGKRVRSKVHDYLQTLVE"
     gene            complement(689..877)
                     /gene="rpsU"
                     /locus_tag="PK849_11900"
     CDS             complement(689..877)
                     /gene="rpsU"
                     /locus_tag="PK849_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPE66874.1"
                     /translation="MTTVVRRDNESLDDALRRFKREVSKVGTLREVRKREHYEKPSEA
                     KKTKRAEAARKRRSKPRG"
     gene            complement(909..1250)
                     /locus_tag="PK849_11905"
     CDS             complement(909..1250)
                     /locus_tag="PK849_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPE66875.1"
                     /translation="MDGKCLFCEMASGAVHVEKVFEDDAVFVIRDISPQAPHHLLVIP
                     KEHVESAADVQNPEVWSKVLDCAVRIASSFGLDNEGYRLVVNCGAKAGQTIPHLHIHL
                     LAGRSFRWPPG"
     gene            complement(1279..2271)
                     /locus_tag="PK849_11910"
     CDS             complement(1279..2271)
                     /locus_tag="PK849_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPE66876.1"
                     /translation="MSVVIELTDCFEILGVQPGAHLREIRSAFRRLALSCHPDIAGPQ
                     KAKQFETIAAAYAQLKSATPAQISESLKKKKSHGDAGKGSPFSWKKTPPRRPKNDRRE
                     ETERENSKRVQDLLLEKALVEAELSMARILEKATRKDEKRTVSSLSKRLLSTHPGVRV
                     LALGALAGRPVEKNVFSSLIEMLRMWSPDDDTVEHLLLLDFSEEQKGDIAAALSARSL
                     LLSEASALALLRWASILPVKMSVVRKLLSHPSPRVLSQALSRWSEKSLPDDLTLIRLL
                     KREEDEVLAPLLRILKDRKVPVWAYGRVKMLAENHVSSAVRVWAQSIVRAQNLV"
     gene            complement(2274..>2552)
                     /locus_tag="PK849_11915"
     CDS             complement(2274..>2552)
                     /locus_tag="PK849_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66877.1"
                     /translation="LRIDIIRSDGRVSSIAPLELGEGVPPSPTETTEEAKGLERRFAR
                     LSVSLSPAQQARGAALLRTMKTLGDGDYSSEAFDSLERMISEMERVVR"
BASE COUNT          564 a          768 c          622 g          598 t
ORIGIN      
        1 cgattacccg cgtctccaaa gcgccgcatg acggacatcg cacagacaca ccccctatat
       61 atagcaatca ccgcgcaata ctactcctat atggtgtatc tttccagaga gacgttgtac
      121 tcctttcata cgggacttta agacgtactg caaagcacaa taaaaaaagc cgggtttccc
      181 cggcttttct aggtctcaaa caattgtgtt gcaacggtca ttcgacaagg gtctgaagat
      241 agtcatgcac ctttgaacga actcgtttac cgtcggcgcg gccttttacg gcagccatga
      301 ccttccccat cacttttccc atatccttgg gtccggcggc tttgacaaac gcgcccgctt
      361 cttcgacaat gtgttccaat tcttcgtcgg agagctgctc aggaagatag gtttcaagga
      421 aaagagcttc cctcagttct tcagccgccc tgtctcctgc accaaccaat gtgtactggt
      481 cggcggcctc acgccgttgt ttcacaaggc gctgaataag ggcgacaatg tcgtcctctg
      541 tcagttccac tgaacgcccc ttctcggctt tggcccgctg cacctccgcc ttgagcatcc
      601 ggagagatga gagttccatc tctttccggg atttcatggc ggcgacaaga tcatccgata
      661 aacgcttctc gagttcaatc acgcggcatt accccctggg cttgctccgt cttttcctgg
      721 cggcttcggc ccgtttcgtc tttttcgcct cactgggctt ttcgtagtgc tccctcttcc
      781 ggacctcccg aagggttccc accttggaga cttcccgttt gaaccgcctg agggcgtcat
      841 cgagggattc gttatctctt cgaacgacag tcgtcataaa cattcccccc tttctttgcc
      901 acccgcatct accccggtgg ccagcggaaa ctacgtcccg ccaacaggtg aatatgaagg
      961 tggggaatcg tctgtcccgc ttttgctccg cagttgacca caagcctgta tccctcgttg
     1021 tcaagaccaa aggatgaagc gatgcggacg gcgcaatcaa ggactttcga ccacacttcg
     1081 gggttttgaa cgtcggcagc cgattcaaca tgctctttcg gtattacgag aagatgatgc
     1141 ggagcctgag gggagatatc gcgaatcaca aatacagcat catcctcgaa gactttttcc
     1201 acatgcactg ctcccgaagc catttcgcag aaaagacatt ttccgtccaa aaatcccacc
     1261 tcacaacgtg acagtattct ataccagatt ttgagcgcgc acaatagact gcgcccaaac
     1321 tcgtacggca gatgacacat gattttcagc gagcatcttc acccggccat acgcccatac
     1381 gggaactttc ctgtctttga gaatccgcag cagcggcgcc aagacttcgt cttcttctct
     1441 cttgagcaac cgaatgagcg tcagatcgtc gggaagcgac ttctccgacc atcgggagag
     1501 cgcttgcgaa agaacccgcg gagaagggtg ggacaaaagt tttcgtacaa cggacatctt
     1561 cacgggcaga atagacgccc accgcagcag agcgagcgcg gaagcctccg agagcaggag
     1621 acttcgtgcg ctcaaggcgg cggcaatatc ccccttctgc tcctcggaga aatccaggag
     1681 aagaaggtgc tccactgtat cgtcgtcggg agaccacatt cgaagcatct ctatcaagga
     1741 ggagaaaacg tttttctcca cagggcgtcc ggcaagagct ccaagcgcaa gcacacgcac
     1801 tccggggtga gtgctcagga gacgtttcga cagcgatgaa actgtccttt tttcatcctt
     1861 ccgcgtcgct ttctcaagaa tcctggccat ggagagctcc gcttcgacca gcgccttttc
     1921 gagaagcagg tcctgaaccc gtttcgagtt ctctcgttcc gtctcttcac gccgatcgtt
     1981 cttcggccgc cggggaggag tttttttcca cgaaaagggc gaaccttttc cggcgtcccc
     2041 atgcgacttc ttttttttca acgactcgga aatttgcgcg ggagttgcgg acttcaactg
     2101 cgcgtaggct gcggcgatag tttcgaactg tttcgccttt tgcggtccgg caatatccgg
     2161 atggcaggag agcgcaagcc tgcggaacgc cgagcggatt tccctcagat gcgctccggg
     2221 ctggacccct agtatctcga aacaatccgt caactcgata acaacgctca tttctatcgc
     2281 acaaccctct ccatttccga aatcatccgt tccagcgagt caaaagcctc ggaggaataa
     2341 tctccgtctc caagggtttt catcgtgcgc agcaacgccg ctcctcgcgc ctgctgcgcc
     2401 ggggaaagcg atacggaaag acgcgcaaac cgcctttcga gccccttcgc ctcctcggtc
     2461 gtctccgtag gtgaaggagg gacgccctct ccaagctcaa gaggcgcgat agaggaaact
     2521 cttccgtcgg aacgtattat atcaattctt aa
//
LOCUS       DAOPKU010000438         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_46902_length_2547_cov_4.504013, whole genome shotgun sequence.
ACCESSION   DAOPKU010000438 DAOPKU010000000
VERSION     DAOPKU010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46902_length_2547_cov_4.504013"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1219
                     /locus_tag="PK849_11920"
     CDS             50..1219
                     /locus_tag="PK849_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPE66878.1"
                     /translation="MTERTDLFVSGPVPMFSSGNEAIAEGALAAGARFYAGYPITPSS
                     EVAEAAARRMPEVGGVYIQMEDELSSMAAIIGASAAGKKAFTATSGPGFSLMQENLGM
                     AAMGEIPCVVVNVQRSGPSTGMATKPAQGDVMQARWGTHGDHGIIALAPSSVQDCYDM
                     TIRAFNLAEKYRTPVVLLADAAIAKLRERAVFRAPSPEEVVERAMPTCPPEEFCPFAV
                     DPEERAITPLPPFGGDYVIRITGSMHDERGGQNATPENADRVIRHLTDKLEKNRADII
                     RVREYALDDAEYALVSFGCSARASRAAVKQARALGKRVGLLELTTLWPFPDDEVRSLL
                     SRVKGAAVVEMNLGQVVREVRRLNDFGIPVLGVHRVDGMLVAPADILSRTEEAFS"
     gene            1216..2181
                     /locus_tag="PK849_11925"
     CDS             1216..2181
                     /locus_tag="PK849_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE66879.1"
                     /translation="MSMLTYLDYLAAHKLPTIWCPGCGNGIVLKAILKAFADLGLDKR
                     KTVAVSGIGCWGWADDYIDANAFHGTHGRALAFATGIKLANPELTVIVLMGDGDGATI
                     GGNHLIHAARRNVDLTAVVVNNFNYGMTGGQYSGTTPAGSKTSTSPLGHAEDPFDLCA
                     LAAAAGAPYVARGSVRDGAALRRLVRNGLSRRGFSFIEALSICPTHYGRRNDARTPFE
                     LYQKLGDMTLPLEKSASVSEEERHGKLLLGTFADKAPDDWTARYKQATKREIFPVCVD
                     RLGRHDSGRTQQATKREASPVCPADACRRLRAVDREQATRKGERS"
     gene            2178..>2547
                     /locus_tag="PK849_11930"
     CDS             2178..>2547
                     /locus_tag="PK849_11930"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE66880.1"
                     /translation="MNARDEFIGIVLAGVGGQGLVTIGTILGEAAVLEGRNACLSSTY
                     GTEARGTFTKSDVLIGDGEIDFIEVVEPDVLLCLAQTAFDRSTPRLEAEGSERTLLLY
                     DSDLVTPSPLCRAKSVGIPFA"
BASE COUNT          427 a          858 c          877 g          385 t
ORIGIN      
        1 gccggagaaa aacccggcat gacgaatacc tatatataag gaggattcca tgaccgaacg
       61 cacagatctt ttcgtttccg gacctgtccc gatgttttct tcggggaacg aggcgatcgc
      121 cgagggcgcc ctcgcggcgg gggcgcggtt ctacgcgggg tacccgatca cgccctcctc
      181 ggaggtcgcg gaggcggccg cgcggaggat gccggaggtc ggcggcgtgt atatccagat
      241 ggaggacgaa ctctcgagca tggcggcgat catcggcgcg tcggcggcgg gcaagaaggc
      301 cttcaccgcg accagcggcc cgggattctc gctgatgcag gagaatctgg gcatggccgc
      361 gatgggcgaa attccctgcg tggtggtgaa cgtccagcgc tccggcccct ccaccggcat
      421 ggcgacgaag cctgcgcagg gcgacgtgat gcaggcgcgc tgggggacgc acggcgacca
      481 cgggatcatc gcgctggccc cctcctcggt gcaggactgc tacgacatga ccatccgcgc
      541 tttcaacctc gcggagaagt accgcacgcc cgtggtgctg ctggccgacg cggcgatcgc
      601 gaagctgcgg gagcgggccg tcttccgcgc cccttccccc gaagaggtcg tcgaacgcgc
      661 gatgccgacc tgtccgcccg aggaattctg tccgttcgcc gtcgacccgg aggagcgcgc
      721 gatcacgccc cttcccccct tcggcggcga ctacgtcatc cgcatcaccg gctcgatgca
      781 cgacgagcgg ggcggacaga acgccacccc ggagaatgcg gaccgagtga tccggcacct
      841 gacggacaag ctggagaaga accgcgccga catcatccgg gtgcgcgaat acgccctcga
      901 cgacgcggag tacgcgctcg tctccttcgg ctgttccgcc cgcgcctcgc gcgcggcggt
      961 gaagcaggcc cgcgccctcg ggaagcgcgt cggcctgctg gagctgacca ccctctggcc
     1021 cttccccgac gacgaggtgc gttcgctgct ctcccgcgtg aagggcgccg ccgtggtgga
     1081 gatgaatctc ggacaggtcg tgcgcgaggt gcggcggctg aacgacttcg gcatccccgt
     1141 cctcggcgtc caccgggtcg acgggatgct cgtcgcaccc gccgacattc tctcccgcac
     1201 ggaggaggcg ttctcatgag catgctcacg tatctggact acctcgcggc gcacaagctc
     1261 cccaccatct ggtgccccgg ctgcggcaac ggtatcgtgc tcaaggccat tctgaaggca
     1321 ttcgccgacc tcgggctcga caagcggaag accgtggccg tctccggaat cggctgctgg
     1381 gggtgggcgg acgactacat cgacgcgaac gccttccacg gcacgcacgg acgggcgctg
     1441 gccttcgcca cggggatcaa gctggcaaac cccgagctga ccgtcatcgt gctcatgggg
     1501 gacggcgacg gcgcgaccat cggcggcaac cacctgatcc acgcggcgcg ccggaacgtc
     1561 gacctcaccg cggtggtggt gaacaacttc aactacggca tgacgggggg gcagtactcc
     1621 ggcacgacac cggcggggag caaaacctcc acgtcgccgc tggggcacgc ggaggacccg
     1681 ttcgatctct gcgcgctcgc cgccgctgcg ggagccccct acgtggcgcg gggctcggtg
     1741 cgcgacgggg cggcgctgcg gcggctcgtc cggaacgggc tctcgcggag gggattctcg
     1801 ttcatcgaag cgctctccat ctgtcccacg cactacggcc gccggaacga cgcgcggacg
     1861 ccgttcgagc tgtaccaaaa gctgggcgac atgacgctcc cgctggaaaa aagcgcgtcc
     1921 gtgagcgagg aagagcggca cggaaagctc ctcctgggca cgttcgccga caaagcgccc
     1981 gacgactgga cagctagata taagcaggcg accaaacggg agattttccc cgtttgcgtc
     2041 gatcgtttag ggcggcatga ttctggccgc acacagcagg cgaccaaacg ggaagcttcc
     2101 cccgtttgtc cggcagatgc gtgccggagg cttcgcgccg tcgatcgcga gcaggcgacg
     2161 cggaaggggg agcggtcatg aacgcgcggg acgagttcat cggcatcgtg ctcgccgggg
     2221 tcggcgggca ggggctggtc accatcggga cgattctcgg cgaagcggcg gtgctcgaag
     2281 ggcggaacgc ctgcctctcc tcgacctacg gcaccgaggc gcgggggacg tttacgaagt
     2341 cggacgtcct catcggcgac ggcgagatcg acttcatcga ggtcgtcgag ccggacgtgc
     2401 tgctctgcct ggcgcagacg gccttcgatc gctccacccc ccggctggag gcggaggggt
     2461 ccgaacgcac gctgctgctc tacgacagcg atctggtgac gccttcgccg ctctgccggg
     2521 cgaaaagcgt cggcatcccc ttcgcgc
//
LOCUS       DAOPKU010000439         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_46915_length_2547_cov_3.135634, whole genome shotgun sequence.
ACCESSION   DAOPKU010000439 DAOPKU010000000
VERSION     DAOPKU010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46915_length_2547_cov_3.135634"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PK849_11935"
     CDS             complement(<1..461)
                     /locus_tag="PK849_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66881.1"
                     /translation="MPAHEKSTVRLGLRHICCFLMLCYVLLVSPSPSAGSAVPQLLPE
                     GETASEDIADRTLPEAATEVLSGDVSVPRAASEDIEKAEAGWGTLSVSLLPEGARTAG
                     ARWSADGGKTWRASGEKILLANADTTVTFKSLVGWKTPETIPVRISNESGA"
     gene            complement(693..2366)
                     /locus_tag="PK849_11940"
     CDS             complement(693..2366)
                     /locus_tag="PK849_11940"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPE66882.1"
                     /translation="MKLTFSWRSALCTLLLAAVMLLPSSLTAEGYVYKVKSGGTGDGS
                     SWERALGEKGLLEKLATAGYGHEFWIAAGTYRPYIAPEPSDSNARADDELDPREHAFV
                     LNRGVALYGGFAGTETSRSQRDWERNTTVLSGYIELQGGSSTNSYTVVVVSDDMGLPA
                     ILDGFSITGGKADGEETLGNGGGMRILDSYTHVTNCTFSGNQATRGGGIYVSGGAPVF
                     KDSTFSGNTADLGGGIYNEESRLTVSDCAFSDNQARSGGAILNNGRETDGAVHALQSA
                     TATITKSTFSGNNTTESGGAISNWKSSPAITDSSFSANKSNVEGGAMFNSDSSPLLTS
                     CTFASNTSENGGAISSWDSSPVIVNCTFYSNRASSNGGALLNDRSSSIVVNATFTRNR
                     ASTGGGMFNFQCSPVVANSILWGNGTEVRSEGGAVTISYSIIEGGFDGEKNLSVNPLL
                     GALHDNGGPTETCALDQKSPAIDAGLFVGSRVSGNVIVPPTDQRGTARPRGAGVDIGA
                     YEYYDEDTPPAPGGGGGGGGCRASSGSAGALLLLFPLVPFLLRRRRTAP"
BASE COUNT          497 a          744 c          759 g          547 t
ORIGIN      
        1 tgcgcaccgc tctcgttgct gatccttacg ggaatagtct ccggagtctt ccagccgacg
       61 aggctcttga aggtgaccgt cgtgtcggcg ttggcaagga gaattttttc cccggaagct
      121 ctccatgttt tgcctccgtc ggcgctccag cgcgcccccg cagtccgcgc gccttcaggc
      181 agcagcgaaa cggaaagcgt tccccacccc gcttcggctt tttcgatatc ctcgcttgct
      241 gcccgcggaa cggaaacatc tcctgagaga acctccgttg cggcctccgg aagagtacga
      301 tcggcgatat cctcgctcgc cgtctctcct tcgggcagca gctgcggaac ggccgaaccc
      361 gcggacggag agggtgaaac caacaaaaca taacagagca tcaaaaaaca acaaatgtgc
      421 cgcaagccca gcctcacggt acttttttca tgcgccggca ttcgatcatc ccctttcgat
      481 gtcggctgga catctccccc gagacatcct cccctgaact gcaagtgtac gatgagtgaa
      541 acggcctgtc aacagaaatc gcaaggcttg aattgcaagg ttgcatgccg gaaaaagcgc
      601 gttacggcgg aaagaacacg ctctccggaa caataggttc cggagagcgc agtgaagtgc
      661 atcatgagga gtatgttgac gaggaactct tgtcaggggg cggttcgacg tcgtcgcagg
      721 aggaacggaa ccagcgggaa gagaaggaga agagccccgg cgctcccgga ggacgcgcgg
      781 catccgccac cgccaccgcc gcctccggga gccggcgggg tgtcttcgtc gtagtactcg
      841 taggcgccga tgtccactcc ggctcccctg ggccgtgcgg ttccgcgttg atccgtcggc
      901 ggaacgatca cgttgcccga aaccctcgag cccacgaaga gtccggcgtc gattgcgggg
      961 cttttctgat ccagagcgca tgtctccgtc ggccctccgt tgtcatgcaa ggctccgagc
     1021 agaggattca cagaaaggtt cttctccccg tcgaatcctc cctcgataat gctgtacgaa
     1081 atagtgacgg cgcctccctc cgagcggact tccgtgccgt ttccccagag aatcgagttg
     1141 gcgacgaccg ggctgcactg gaagttgaac atcccgccgc ctgtggacgc cctgtttctt
     1201 gtgaaggtcg cgttgacgac gatggagctg cttctgtcgt tgagcagcgc gccgccgttg
     1261 gatgatgccc tgttcgagta aaatgtacag ttgacgatga cggggctgct gtcccagctc
     1321 gagatcgctc cgccgttttc ggatgtgttt gaagcgaacg tgcaggatgt caggagcggc
     1381 gaactgtcgc tgttgaacat ggcgcccccc tcgacgttcg acttgtttgc cgaaaacgaa
     1441 gagtccgtga tggcggggct gctcttccaa ttggagatgg cgccgccgga ctccgtggtg
     1501 ttgtttcctg agaacgtcga tttcgttatg gttgccgtcg cgctctgaag agcgtggact
     1561 gctccgtctg tctcacgccc gttgttcagg atcgccccgc cggatcgcgc ctggttgtcc
     1621 gagaatgcgc agtcggagac ggtaagacgg ctctcttcgt tgtaaatgcc gccgccgagg
     1681 tctgccgtgt tgcctgaaaa tgtggagtcc ttgaagacag gagcgcctcc gctgacataa
     1741 atgccgccgc cgcgtgtcgc ctggttcccg gagaacgtgc agttcgtgac gtgggtatag
     1801 ctgtcgagaa tgcgcatgcc gccgccgttg ccgagggttt cttcaccgtc cgctttgcct
     1861 ccggtgatgc tgaagccgtc cagaattgcg ggaagcccca tgtcgtcgct gacaacgacc
     1921 acggtgtagc tgtttgtcga gctccctccc tgcagttcga tgtaccctga aagcactgtg
     1981 gtattccgtt cccagtcgcg ttggcttctt gacgtttcgg ttccggcgaa tcccccgtag
     2041 agcgcgactc ccctgttgag gacgaaagca tgttcgcgag ggtccagttc gtcgtcggcc
     2101 cgagcgtttg aatcgctggg ctcaggagcg atatacggcc ggtatgtgcc tgcggcgatc
     2161 cagaactcgt gcccgtatcc cgccgtcgcc agcttttcga gaagtccctt ttcgccaagc
     2221 gccctttccc atgaggaacc gtctccggtt ccgccggatt ttaccttgta gacatacccc
     2281 tcggctgtaa gagaggaggg gagaagcata accgccgcaa gcagcagcgt acagagcgcc
     2341 gaacgccatg aaaaagtgag tttcattata aacgaccacc ttcctaaaga aagctgcgat
     2401 cccctgaaga gtgtgttgca agactctttg aggcgtcgac tgtgtaaaat tatactatac
     2461 ggttttgtaa gaggaaggaa gatgtgttcc gtcagggagc gggatgaaag ttgaggaaag
     2521 aaaacacggc gtccggcctt tgtgggc
//
LOCUS       DAOPKU010000440         2545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_46955_length_2545_cov_4.634538, whole genome shotgun sequence.
ACCESSION   DAOPKU010000440 DAOPKU010000000
VERSION     DAOPKU010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46955_length_2545_cov_4.634538"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /gene="rlmN"
                     /locus_tag="PK849_11945"
     CDS             complement(<1..973)
                     /gene="rlmN"
                     /locus_tag="PK849_11945"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPE66883.1"
                     /translation="MNESVNALDLSYEEWIETSVTRWKLPRYRADQLCQWIYDKKVFN
                     VHEMTNLSKDLREKLVYELMILPPIAVREDTSKDGTKKFLWQLYDGERIESVLLSHGS
                     HMTACISSQVGCPLNCSFCATGKSGFTRNLSAGEIVGQFLAMEKRIGENINNVVYMGM
                     GEPFLNSAEVFKSIRILNSSKMRNLGARHITISTSGVVPGILELADFPIPVRLSVSLH
                     APNDVLRSKIMPVNRKHSLGSLIDALKAYKDKTGERITIEYVMLQKVNDEPEHAYEMA
                     AILSGLGVYVNLIPYNPIALLQPGEQGFQRSTTERIKEFAAILAKLGI"
     gene            complement(1121..>2545)
                     /locus_tag="PK849_11950"
     CDS             complement(1121..>2545)
                     /locus_tag="PK849_11950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE66884.1"
                     /translation="WKRGRAKVEIGLAKGKKLEKTNFFLVAQMDYGEFIAPFIGNMVY
                     FVTVSAVLLFFMLSISRKLYKKVDRSLNLLFEGVIKTEQGEYDSLDPELLEEAPFELR
                     ELGIAFSSMAETVRSKTAELEFRSFHDELTGLYNRAYFEDAMRRFDSGRFNPVTVVVC
                     DVNGLKMINDTLGHKAGDELIVAAAHSLANAGRKTDIVARIGGDEFAMLLPESTEETA
                     GEVLRRIRAEIDKYKRGEDSLPLNIACGAATTSGRIQNIEALLSEADKQMYASKQLEK
                     PHSKREILEYLSSRIHDLRKASHDRKRHMAACEAIMEAFVREQPDIAPEKKEFLILLA
                     KNHDIGFTEIPSEITEKPGPLTDEEYLLVQRHPETGARIASLFPELADLAEPIRLHHR
                     WWNGKGYPAGDGGEAIPLESRLMAIVDAYEAMTGDKIYRTPLSPEEALKELHRCAGSQ
                     FDPVWTERFITFFREYREREHIFA"
BASE COUNT          507 a          703 c          705 g          630 t
ORIGIN      
        1 cgatgccgag tttggcaaga atggctgcga actccttgat gcgctccgtc gtcgaacgct
       61 gaaatccctg ctcccccggc tgcaggagcg cgatcggatt gtatggtatt aggttcacgt
      121 agacgccgag ccccgaaaga atggcggcca tttcgtaggc gtgctcaggc tcgtcgttga
      181 ctttttgcag catgacgtat tcgatcgtga tccgttcccc tgtcttgtct ttgtaggcct
      241 tcagcgcgtc gatgagcgag cccagagagt gcttcctgtt caccggcatt attttgctcc
      301 gcagcacgtc gttcggcgcg tggagggaga cggaaagacg caccggaatc gggaagtccg
      361 ccagttccaa aatgcctgga acgacgccgg atgtcgagat tgtgatgtgg cgcgccccca
      421 ggttgcgcat ctttgacgaa ttcagaatcc ggatcgactt gaaaacttcg gcggaattca
      481 ggaaaggttc gcccatcccc atatagacca cgttattgat gttttctcca atgcgttttt
      541 ccatcgccag gaactgcccg acgatctcgc cggcgctgag gttgcgcgtg aaacccgatt
      601 ttcctgtggc gcagaaggag cagttcagag gacaccccac ctggctggag atgcaggctg
      661 tcatatgcga gccgtgcgac agcaggacgg attcgatccg ctctccgtcg taaagctgcc
      721 agaggaattt tttcgtgccg tccttcgacg tatcctcgcg gacggcgatc gggggaagga
      781 tcatcagttc gtaaacgagc ttctcccgca gatccttcga gaggttggtc atctcgtgaa
      841 cattgaagac ttttttgtcg tagatccact ggcagagctg gtcggctctg tagcgtggaa
      901 gcttccacct tgtgacgctt gtctcgatcc actcttcgta cgagagatcg agggcgttaa
      961 cgctttcgtt cataccgctg cctcctgagg cgttcaagaa cgccgtttca attctaacac
     1021 tgaatcatgt catggaaaga tgctctctgt caatgcctgt tccgccgaat tctctaggaa
     1081 aacccatgca gggtacggaa tctctccgga tgccggccgt tcatgcaaag atatgttctc
     1141 tctcccgata ctcgcgaaaa aacgtaataa aacgttcggt ccatactggg tcgaactggc
     1201 tcccggcgca ccggtgaagc tctttcagcg cttcttccgg ggagaggggc gttctgtaga
     1261 tcttgtctcc cgtcatggct tcataggcgt cgacgatcgc catgaggcgg ctttcgagcg
     1321 ggatcgcctc tcctccgtct cctgcggggt accctttccc gttccaccat cggtggtgca
     1381 gacggatagg ctccgccagg tcggcaagtt ccggaaagag cgaagcgata cgcgcccctg
     1441 tctccgggtg gcgctggacg agcaggtatt cttcgtccgt caacggtccc ggtttttcgg
     1501 taatctccga cgggatctcg gtaaacccga tgtcgtggtt ttttgcgagg agaatgagga
     1561 actctttttt ctcgggagct atgtccggct gctctcggac gaacgcttcc atgatcgcct
     1621 cgcacgccgc catgtgtctc tttctgtcgt gagacgcctt ccggaggtcg tgtattctgg
     1681 aagagagata ctccagaatt tctcgtttgg agtgcggttt ttcaagctgt ttggatgcgt
     1741 acatctgctt gtcggcctca cttaaaagag cctcgatatt ctgtatccgg cctgaggtgg
     1801 ttgccgcccc gcacgctatg ttcagcggaa gcgaatcttc gccgcgctta tacttgtcga
     1861 tctcggcccg aatacgccgg agtacctctc ctgctgtttc ttcagtagat tcgggaagga
     1921 gcatcgcgaa ttcgtcgcct ccaatcctgg cgacgatatc cgttttccgg ccggcattcg
     1981 ccaacgagtg cgccgctgcg acgataagct cgtcgccggc cttgtgtccg agagtatcgt
     2041 tgatcatttt gaggccgttc acatcgcaga ccacaacggt tacaggattg aaccggccgg
     2101 agtcgaagcg gcgcatagcg tcttcgaaat acgcgcggtt atacaggccg gtcaattcat
     2161 cgtggaagga gcgaaactcc agctcagcag tcttgcttcg cactgtctcc gccatcgagc
     2221 tgaaggcgat tccgagctcc cgcagttcaa agggggcctc ctccagcagc tcagggtcga
     2281 gagagtcata ctctccctgt tcggttttta ttaccccttc gaagagcagg ttgagcgatc
     2341 tgtcgacctt cttgtacagc ttgcgagaga tagagagcat gaaaaaaagg aggactgctg
     2401 aaaccgtaac gaaatagacc atgttgccga tgaatggagc aatgaattca ccgtaatcca
     2461 tttgagctac aaggaaaaag ttcgtttttt ccagtttctt gcccttggcg aggccaatct
     2521 cgaccttggc acggccgcgc ttcca
//
LOCUS       DAOPKU010000441         2543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_47003_length_2543_cov_3.342042, whole genome shotgun sequence.
ACCESSION   DAOPKU010000441 DAOPKU010000000
VERSION     DAOPKU010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47003_length_2543_cov_3.342042"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PK849_11955"
     CDS             complement(<1..404)
                     /locus_tag="PK849_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE66885.1"
                     /translation="MENKKLLLDIQDLSIQYDTEGGTVHAVEHLNLTLEHGENLGFVG
                     ETGAGKTTTALGIMGLIPNPPGIIADGKIFFEGEDLLKKSERQMRDIRGGKIAMIFQD
                     PMTSLNPVMTVDKQIAEMVLLHNDVSEQQALER"
     gene            complement(423..1295)
                     /locus_tag="PK849_11960"
     CDS             complement(423..1295)
                     /locus_tag="PK849_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66886.1"
                     /translation="MSGNTAPRMKSEAGLLDIWRRLRKNPLAMFGLFIVVLLIFFAIT
                     ADVIAPYKYYEQNLINSFQSPSKEHLFGTDEFGRDILSRIIHGSRISLQVGLIAVGIS
                     VIFGGFFGAVAGYYGGRIDNLIMRGMDILLSIPSILLAIAIAASLGPGLYNMMVAVGI
                     STTPQYARIIRGSVLSLRGQEFVEAAKAVGSSDTRIIMKHILPNCLAPIIVQSTLGVA
                     SAILTAAGLSFIGLGIQPPTPEWGAMLSGGREYIRDYAYMTVFPGLAIMITILALNFL
                     GDGLRDVLDPKLKR"
     gene            complement(1309..2238)
                     /locus_tag="PK849_11965"
     CDS             complement(1309..2238)
                     /locus_tag="PK849_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE66887.1"
                     /translation="MLKYIVKRIFSLIPVLLGVAFIVFTLLYLTPGDPARIVLGEQAT
                     EEALAEFREKEGLNDPFLVQFGNYVWKAVTKGDIGRSYMTRRSVANEIMSAFPSTLKL
                     ALSCVIAAVLLGIPIGILSAIRQNTLFDNITRFLAIVGLSMPVFWQGVLLILLFSVKL
                     RWFPASGFDTWATLVLPTLTLACNPLAVITRMTRSSMLEVVRQDYIRTARAKGQTEQI
                     VIWRHALGNALIPIVTVIGLQFGSLLAGAVLTESIFSIPGVGRLMVDAIKMRDYPVVQ
                     GGVLFIAVAFSFVNLMVDLLYAWIDPRIRAQYR"
     gene            complement(2342..>2543)
                     /locus_tag="PK849_11970"
     CDS             complement(2342..>2543)
                     /locus_tag="PK849_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE66888.1"
                     /translation="IDQGKTMPDRPEREALYKKLQQLVGDARPMLYLYNEENFFGVNK
                     NVQNFRPSPRGYHNVTGVTFKE"
BASE COUNT          561 a          695 c          762 g          525 t
ORIGIN      
        1 cgttcaagcg cttgctgctc gctgacgtcg ttgtggagga gcaccatctc cgcgatctgc
       61 ttatccaccg tcatgaccgg attcagcgaa gtcataggat cctgaaagat catcgcgatc
      121 ttgccgccgc ggatatcgcg catctggcgt tcgctttttt ttaagaggtc ttcgccctcg
      181 aaaaagatct tcccgtccgc gattatgccc ggaggattgg ggatcagccc catgataccg
      241 agcgccgtag ttgttttccc ggcgcccgtt tcgcctacaa aaccaaggtt ttcgccgtgt
      301 tcgagcgtga gattgagatg ttcgacggcg tgcaccgtgc cgccttccgt atcgtactga
      361 atgctcagat cctgtatgtc cagaagaagt ttcttgtttt ccatagtcgt cgcctccgcc
      421 ttttatcgct tcagcttcgg gtcgagcacg tcgcgcagac cgtcgccgag gaagttgagc
      481 gccaatatgg taatcatgat ggcaaggccg gggaagaccg tcatgtaggc gtaatcgcgg
      541 atgtactcgc gtccgccgga gagcatggcg ccccactcgg gcgtgggcgg ctgaatgccc
      601 aaaccgatga aggagagacc ggccgccgtc aaaatggcgc tcgccacacc gagcgtcgac
      661 tggacgatga tcggcgcaag gcagttgggg aggatgtgtt tcatgatgat ccgggtgtcg
      721 gaagaaccga cggccttggc tgcctcgacg aactcctgcc ctcgcagcga gagcaccgag
      781 ccccggatga tgcgcgcata ctgcggcgtg gtcgaaatgc cgacggcgac catcatgttg
      841 tacagtcccg gcccgagcga ggcagcgatg gcgatggcga gaagaatcga tggaatggaa
      901 agaaggatgt ccatccctct catgatgagg ttgtcgatgc gccccccgta gtatccggcg
      961 acggctccga agaaaccgcc gaaaatgacc gagatgccga ccgcgatcag tccgacttgg
     1021 agcgagatgc gcgagccgtg gataatgcgg ctgagaatat cgcgcccgaa ttcgtccgtg
     1081 ccgaagagat gctcctttga aggagactgg aaggagttga tcaaattctg ttcataatac
     1141 ttgtacggcg cgatgacgtc ggctgtaatg gcaaaaaaga tgagaaggac gacgataaaa
     1201 aggccgaaca ttgccaaagg gtttttgcga aggcgtctcc agatgtccag gagccctgcc
     1261 tcgctcttca ttcgaggtgc cgtatttcca ctcattttct ttcctcccct acctgtactg
     1321 cgctctgatt ctcgggtcga tccaggcgta gaggaggtcg accatgagat tcacgaagct
     1381 gaaagcgacg gcgatgaaca ggacgccacc ctgaacgaca ggatagtccc gcatcttgat
     1441 ggcatccacc ataaggcgcc caactccggg gatggagaat atggactcgg tgagcaccgc
     1501 gcctgcgagc agcgaaccaa actggagacc gatgaccgta acgatgggga taagcgcgtt
     1561 gccgagagca tgtctccaga taacgatctg ctccgtctgt ccttttgccc gcgccgtgcg
     1621 aatgtagtcc tggcggacca cttcaagcat gctcgaacgg gtcattcgcg taatgacggc
     1681 gagaggatta caggcgagcg taagggttgg aagcacaagc gtcgcccacg tgtcgaaacc
     1741 ggaagcgggg aaccagcgca gtttcacgga gaagagcaga atcaggagca cgccctgcca
     1801 gaagaccggc atggaaagcc cgacaatcgc gaggaaacgc gtgatgttgt cgaagagcgt
     1861 gttttgacga atggccgaaa gaatacctat cggaattccg agcagcacag cggcgatcac
     1921 gcagctcagc gcgagtttca gggtggacgg gaacgcgctc ataatctcgt tcgccacgga
     1981 acggcgcgtc atgtaggaac gcccgatatc acccttggtg acggccttcc agacgtagtt
     2041 gccgaactgg acgaggaacg ggtcgttcag cccctctttc tcgcgaaact cggcgagcgc
     2101 ttcttcggtt gcctgctcgc ccaacacgat gcgtgccgga tcacccggcg tcaggtagag
     2161 cagcgtgaag acgatgaagg ccaccccgag caacaccggg atcagcgaaa aaattcgttt
     2221 cacaatatac ttcagcaatg gactatccct cctggacatc cccgcagcgg cctgcgggaa
     2281 gcaaggcaaa gcaggagcgg aacgctcctg ctttgccggt agaatcggtt attttttctt
     2341 cttattcctt gaaggtgact ccggtaacat tgtggtagcc tctggggctt gggcggaagt
     2401 tctggacgtt cttgttcacg ccgaagaagt tctcttcgtt gtagaggtag agcatgggac
     2461 gggcgtcgcc gacaagctgc tgcagcttct tgtaaagagc ttctctttcg ggtcggtcgg
     2521 gcatcgtctt gccctggtcg atg
//
LOCUS       DAOPKU010000442         2533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_47258_length_2533_cov_4.635593, whole genome shotgun sequence.
ACCESSION   DAOPKU010000442 DAOPKU010000000
VERSION     DAOPKU010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47258_length_2533_cov_4.635593"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="PK849_11975"
     CDS             <1..1936
                     /locus_tag="PK849_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPE66889.1"
                     /translation="GSPAPEKRYYMWQPPLVRGGGFRISPAREATGLIAQLVLRKSGF
                     LAFCKSRNAVEVVLKESRDHLKAEGRALSGEDLSDLISGYRGGYTPLERRAIEDKMAG
                     GKLRGLVATNVLELGIDIGRLDTAVLVGFPGTRASFWQQAGRAGRKGAKSSVFLLLDN
                     LPLDQYLAINPEWLFSGESESAVVDPDNLFIQLAHVRAAAAELPLSPDDLALFPDLGE
                     IVPVLFSSKELRKEAGKFFWSGGVYPSGDFSLRNMDRVRYKLKLGADGSLLTEMDELQ
                     AFREIYRGAIYMHEGRQYLVERMDTKNTEALAKPVDGNYYTVSCDTTEVRKLQELKRS
                     AAGRTTRRFGDVRVSYTTLGFRRQQFHNHQNLGYEALERPLSKTFDTEGFWIRLPDEV
                     RSLFLALTPQEENRPPMQFWKTYAEGLGFALLNATMMATMTTHDDIGSALLADTDGEG
                     GGMSVCVFDMYAGGLGFADKGYDRVSEIIANAALMVEGCACTDGCAACVGDYHLDKAV
                     ILWGLKSLLEELPAPKTLRRRTPRASAQQPEKPFRMETLADEWGAFREALLSSGDALG
                     HFLASVPAARTDGSRLVLSLRHEFSAEWLSEATYREQLEALIRRFAHVPEDFSVGYEI
                     LNGDASIASKLGKMHRDLAR"
     gene            1976..>2533
                     /locus_tag="PK849_11980"
     CDS             1976..>2533
                     /locus_tag="PK849_11980"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPE66890.1"
                     /translation="MNEQQRRAVLDESRAVLVNAAVGSGKTTVLIAKALYEHAVCGVP
                     LREMAVLTFTNKAADEIRERMRAADPEARDEETAWFGTFHSVALRLLQQALPVGELGF
                     TPAFTVLAPDEELELAERLICEHGLCVKYRNKLGKRLEAFASGRSLYGVMKREDDVGR
                     LCELLAAEKLRLNAMNFDDLLKNAAR"
BASE COUNT          450 a          795 c          897 g          391 t
ORIGIN      
        1 cggctctcca gcgcccgaga agcgctacta tatgtggcag ccgcctctcg tgcggggagg
       61 aggcttccga atctccccgg cccgcgaggc gacggggctc atcgcgcagc tcgtcctgcg
      121 gaagtccggc tttctggcgt tctgcaagtc gcgcaacgcg gtggaggtgg tgctgaagga
      181 gtcccgcgac catctgaagg ccgaagggcg cgcgctttcg ggggaggacc tctccgacct
      241 gatctccggc tatcggggcg gctacacgcc tctggaacgg cgcgccatcg aggacaagat
      301 ggcgggcggt aaactgcgcg ggcttgtggc gaccaacgtg ctggagctgg gcatcgacat
      361 cgggaggctg gacacggccg tgctcgtggg cttccccgga acgcgcgcct ccttctggca
      421 gcaggcgggg cgcgcaggac ggaagggggc gaaatcctcc gtttttttgt tgctggacaa
      481 cctgccgctg gaccagtacc tcgcgatcaa tccggagtgg ctcttctcgg gggagagcga
      541 aagcgccgtc gtcgacccgg acaacctctt catccagctc gcccacgtgc gggccgccgc
      601 cgcggaactc cccctctcgc cggacgatct cgcgctcttc ccggatttgg gggagatcgt
      661 gccggtgctc ttctcgtcga aggagctgcg gaaggaagcg gggaagttct tctggtcggg
      721 cggcgtctac ccctccggcg acttcagcct gcgcaacatg gacagggtgc gctacaagct
      781 gaagctcggc gccgacggct cgctgctcac ggagatggac gaactgcagg cgttccgcga
      841 gatctacaga ggcgcgatct acatgcacga agggcggcag taccttgtgg agcggatgga
      901 cacgaaaaac accgaagcgc tggcgaagcc ggtggacggg aactactaca cggtctcctg
      961 cgacacgacc gaggtgcgca aactccagga gctgaagcga agcgcggcgg gcagaaccac
     1021 gcgccgattc ggcgacgtgc gcgtctcgta caccacgctc ggcttccgcc gccagcagtt
     1081 ccacaaccat cagaacctgg ggtacgaggc gctggagcgc ccgttgagca agacgttcga
     1141 caccgagggg ttctggatcc gccttcccga cgaggtccgc tccctcttcc tggcgctcac
     1201 cccgcaggag gagaaccgcc ccccaatgca gttctggaag acgtacgccg aggggctggg
     1261 gttcgccctg ctcaacgcga cgatgatggc gacgatgacg acgcacgacg acatcggcag
     1321 cgcgctgctg gcggacaccg acggagaggg aggcggcatg tcggtctgcg ttttcgacat
     1381 gtacgccgga ggactgggct tcgcggacaa ggggtacgac cgcgtatcgg agatcatcgc
     1441 caacgcggcg ctgatggtcg aggggtgcgc gtgcacggac ggctgcgccg cgtgcgtggg
     1501 cgactaccat ctggacaagg cggtgatcct ctgggggctg aagagcctgc tcgaagaact
     1561 ccccgcgccg aagacactca ggaggagaac gccgcgcgcc tccgcgcagc agccggagaa
     1621 gccgttccgc atggagacgc tggcggacga gtggggcgcg ttccgggagg cgctcctctc
     1681 ctcgggcgac gccctcgggc attttctggc gagcgtgccg gcggcgcgga ccgacgggag
     1741 caggctcgtc ctgtcgctgc ggcacgagtt ctccgcggag tggctgtcgg aggcgacgta
     1801 ccgggagcag ctcgaagccc tgatccggag gtttgcgcac gttccggagg acttttccgt
     1861 ggggtacgaa atcctgaacg gcgacgcgtc gatcgcgtcc aagctcggga agatgcacag
     1921 ggatcttgca cgataggggg aaccgacgat cgacggcggc gagatacttc aaagactgaa
     1981 cgaacagcag cggcgggccg tgctcgacga gagccgggcc gtcctggtga acgccgccgt
     2041 cggcagcgga aagaccaccg tcctcatcgc caaggcgctg tacgagcacg cggtctgcgg
     2101 cgtgccgctg cgggagatgg cggtgctcac cttcaccaac aaggcggcgg acgagatcag
     2161 ggagcggatg cgcgccgccg acccggaggc ccgagacgag gagacggcct ggttcggcac
     2221 gttccacagc gtggccctgc gcctgctgca acaggcgctt cccgtcggcg agctgggctt
     2281 tacgcccgcc tttaccgtcc tcgctcccga tgaagagctg gagttggccg aacgcctgat
     2341 ctgtgagcac gggctgtgcg tcaagtaccg gaacaagctg ggcaaacgct tggaggcgtt
     2401 cgcgtccgga cgaagcctct acggcgtgat gaagcgcgag gatgacgtcg gcaggctctg
     2461 cgagctgctc gccgcggaaa agctccggct gaacgcgatg aacttcgacg acctgctgaa
     2521 gaacgcggcg cgc
//
LOCUS       DAOPKU010000443         2533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_47261_length_2533_cov_4.236077, whole genome shotgun sequence.
ACCESSION   DAOPKU010000443 DAOPKU010000000
VERSION     DAOPKU010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47261_length_2533_cov_4.236077"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..834)
                     /locus_tag="PK849_11985"
     CDS             complement(85..834)
                     /locus_tag="PK849_11985"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPE66891.1"
                     /translation="MSIRCDVVLSPSETLPEADCWLVIDILRATTTMCAYFDGGGKRL
                     YPAESIEEAFTLRDRLAREGNAPLLMGERNSLPPPGFDLGNSPLELLAAAPATRPEAV
                     MATTNGTRALLKAASSGAKVYPVCARNANATLRAALGAGERIGILCAGLYGRPAMDDA
                     ACAGLLAEQILGQGAVLDDGAKMALSVWQAGMRNLSALLGASVHGGRLLSLGFAPDMA
                     YAAEIDASSSVLVLSEVKELPCIVPGNSPAA"
     gene            complement(831..1571)
                     /locus_tag="PK849_11990"
     CDS             complement(831..1571)
                     /locus_tag="PK849_11990"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPE66892.1"
                     /translation="MGRPVIGIVGNLLIEQAGFSPGMERSYVNNDYVAAVERAGGIPF
                     ILPVVRGQDVAERQTECVDALLISGGYDMDPLLYGEEPIRALEYVYRETDIFQLRTIK
                     TALKRGQPILAICKGIQLLNVACGGTLYQDLGEIPGSFVKHSQNRKRSCPSHTVIAEG
                     ESLIAELFGGAFPVNSYHHQALKEIGRGLVVTSRAMDGVVESVETEDDSFVVGVQWHP
                     EMMLSGGDEMLPLFLRFAEAAERFALSR"
     gene            1755..>2533
                     /locus_tag="PK849_11995"
     CDS             1755..>2533
                     /locus_tag="PK849_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66893.1"
                     /translation="MSRILYSFSVIAFGLLLGYGVRLASERGLVRLRWSMPVLRTFLQ
                     RLALLGLGPFTFIGALWVVEIREPRLALLAGIGAFCHIFGGLAGALLARLFRLERPQA
                     GAMFCCGFFTNIGAIGGLVTFVFLGEAGYAYVPLFKLFEDMVYYGVGFPVARLYSDTK
                     TTGGVSLAARLARDPFLLVSIFSLTTGTTLNLLNVPRPEVFSQLNALLIPLTTVILLA
                     SIGMAMRFGKTSAYLKEGVAILGVKFLLVPAVCTSLAALLGL"
BASE COUNT          422 a          822 c          810 g          479 t
ORIGIN      
        1 ctcatcgaaa cggagacgga gaagaagatg cgtgagctgc tccagaaata caatatcgag
       61 tagaacgcgc cactcctcga cgaatcaagc ggcaggggag tttccgggaa cgatgcaggg
      121 gagttccttc acctcgctca gtacgagcac ggaactggag gcgtcgatct cggcggcgta
      181 ggccatgtcc ggggcgaacc ccagcgagag caggcgtccg ccgtgcaccg acgccccgag
      241 cagcgcggag aggttgcgca ttccggcctg ccatacggag agcgccatct tcgcgccgtc
      301 gtccagcacc gcgccctgtc cgaggatctg ttcggcgagc aaccccgcgc aggccgcgtc
      361 gtccatggcc gggcgtccgt agagccctgc gcagaggatg ccgatacgct ctccggcgcc
      421 gagcgccgca cggagcgtcg cgttcgcgtt ccgcgcgcac acaggataga ccttcgcccc
      481 gctcgacgcg gccttcagca gcgccctcgt gccgttggtc gtcgccatca cggcttccgg
      541 gcgcgtcgca ggcgcggcgg caagcagctc cagaggggag ttcccgaggt cgaagccggg
      601 gggaggaagc gagttccgct cgcccatcag gagcggggcg ttcccctccc gtgccagcct
      661 gtcgcgcagc gtaaaggcct cctcgatgga ctccgccgga tagaggcgct tgccgccgcc
      721 gtcgaagtag gcgcacatcg tcgtggtggc cctcagaatg tcgataacga gccagcagtc
      781 ggcctccggg agcgtctccg agggcgagag gacgacgtcg cagcgtatgc tcatcgcgag
      841 agcgcgaagc gttccgccgc ctccgcgaaa cggaggaaga gcggcagcat ttcgtcgccg
      901 ccggagagca tcatctcggg gtgccactgc acgccgacaa cgaaagagtc gtcctcagtc
      961 tccaccgact cgacgacgcc gtccatcgcg cgcgaggtga cgacgagtcc gcgccctatc
     1021 tccttgagcg cctggtggtg gtagctgttc acaggaaagg cgcccccgaa gagttcggcg
     1081 atgagcgact ccccctcagc gatcacggtg tggctggggc aggagcgttt tctgttctgc
     1141 gagtgtttga cgaaggagcc ggggatctcg ccgagatcct ggtagagcgt ccctccgcag
     1201 gcgacgttca gcagctggat tcccttgcag atggcgagga tgggctgccc ccgtttcagc
     1261 gccgtcttga tggtgcgaag ctggaagatg tcggtctccc tgtagacgta ctcaagggcg
     1321 cgtatcggct cctccccgta cagcagcgga tccatgtcgt acccgccgga gatgagcagc
     1381 gcgtcgacgc actccgtctg gcgttcggcc acgtcctgtc cgcgaacgac gggcagaatg
     1441 aacgggatgc ctcccgcgcg ctccaccgca gccacatagt cgttgttcac ataactccgt
     1501 tccatgccgg gcgaaaaccc agcttgctcg atgagcagat ttcccacaat gccgatgacc
     1561 ggacgaccca ttgccttccc ctcctggtgc gcggcttctg cgccgcatgt gtgtgattca
     1621 agtatagcgc gccgggcgcg ttttttcacg ttgacatcgg gagcttcacg gggaataata
     1681 cccttcatgc gaatcaagga ggagttcttt gtgaagtcgc tctataatat cgaaaaaaag
     1741 aacgaagccg gagtatgagc cggatacttt attccttttc cgtcatcgcc ttcgggctgc
     1801 tgctggggta cggcgtccgg ctcgcgagcg agcgggggct cgtccggctg cgatggtcca
     1861 tgcccgtgct ccgaaccttt ctgcagcggc tcgccctgct gggcctgggt ccgttcacct
     1921 tcatcggggc gctctgggtg gtggagatac gcgagcctcg ccttgccctg ctcgccggaa
     1981 taggcgcttt ctgccacatc ttcggcggcc ttgcgggcgc gctgctggcg cgtctcttcc
     2041 ggctggaacg accgcaggcg ggagcgatgt tctgctgcgg attcttcacc aatatcgggg
     2101 ccatcggcgg actggtgacc ttcgtctttc tcggggaggc ggggtacgcc tacgtcccgc
     2161 tcttcaagct gttcgaggac atggtctact acggcgtcgg cttccccgtg gccaggctct
     2221 acagcgatac gaagacgacg ggcggcgtaa gcctcgctgc acggctggcg cgcgatccgt
     2281 tccttctcgt ctccatcttc agcctgacga cgggaacaac gctgaatctg ctcaatgtgc
     2341 cgcgccccga ggtcttctcg cagctcaacg cgctgctcat tccgctgact acggtgatcc
     2401 tgctggcctc catcggcatg gccatgcgct tcggcaagac ctccgcctac ctgaaagaag
     2461 gcgtcgccat tctcggcgtg aagtttctgc tggttccggc cgtctgcacg tcgctcgccg
     2521 ccttgctggg gct
//
LOCUS       DAOPKU010000444         2517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_47664_length_2517_cov_4.175467, whole genome shotgun sequence.
ACCESSION   DAOPKU010000444 DAOPKU010000000
VERSION     DAOPKU010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47664_length_2517_cov_4.175467"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PK849_12000"
     CDS             complement(<1..1015)
                     /locus_tag="PK849_12000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE66894.1"
                     /translation="MNDDRTPYDALGALFAKILEPVALYRALFDERGAFRDVVYIDVN
                     PAYEQVMGIRKEDILGRPFREIWSEREREWQDIILQVATTGIYARYEGPSYDTGKYLH
                     AIAFSPTRSVVAVIFLDMTDWKRAEEALQEKEVLLTEYRQELRTLAARLSLAEEHTRR
                     EIAVKLHDRMGYSLVSLLTRLRTLAEKHDPDDIAPAIEEVEGLLSDTRSLTLEISSPL
                     LYEVGLEPALASLAERMLAPHGIAVDFRECGPQSHISMEIRILMYQMAQELLLNVIKH
                     AGARNVILRVQRGNKRVRMLVEDDGAGFSSKTPKHWGKWGGMGLFSIREKLHSVGGEI
                     RVFS"
     gene            1335..>2517
                     /locus_tag="PK849_12005"
     CDS             1335..>2517
                     /locus_tag="PK849_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HPE66895.1"
                     /translation="MKLEIGKFFVKEMVFGEKTGYSKGVLTINKEEALAVVKEDSHIT
                     EAELHIACPGDEVRMVPVKEAIEMRCKVSGGVMFPGVTNPVMPAGQGRTHALKDCSLI
                     VVGKHWGSFQDGLIDMAGPFQRNTIFGSLKNLVLVGDTDEEFERHEQQKKNRALRWAG
                     HRLAEYVGKAVAALEPEEIETWELEPVNRRDASVQGLPSVVYVLQPQTQMEAMGYNTL
                     VYGWDGNHMLPTFMHPNELLDGALISGSFMPVSSKMSTYEHCNNPVVKRLMKEHGKTL
                     NFLGLVMSNLNVAMDQKQRSAYMVAQMAKNLGADGAIVAEEGYGNPDVDYIQTIVELE
                     KVGIKTVGISNECTGRDGGSQPLVALDPAANALVSTGNVSQYFELPPMKTVLGELESM
                     AR"
BASE COUNT          479 a          797 c          792 g          449 t
ORIGIN      
        1 ccgagaagac gcgaatctct ccgccgacgg agtgcagttt ttcccggatg ctgaagagcc
       61 ccatgccgcc ccacttcccc cagtgcttcg gcgtcttgga ggagaacccc gcgccgtcgt
      121 cctcgacgag catgcggacg cgcttgttcc cccgctgcac gcgaaggatc acgttccttg
      181 ccccggcgtg cttgatcacg ttgagcagca gctcttgcgc catctggtac atcagaatgc
      241 ggatttccat cgagatgtgc gattgcgggc cgcactcgcg gaagtcgacg gcgatgccgt
      301 gcggggcgag catccgttcg gcgagcgagg cgagcgccgg ttccaggccg acttcataga
      361 gcagcggcga gctgatctcc agcgtcaggg agcgcgtgtc ggagagcagc ccctccacct
      421 cctcgatggc cggggcgatg tcgtcggggt cgtgcttttc ggcgagggta cgcaggcgcg
      481 tgagcagcga gacgagcgag taccccatcc ggtcgtgcag cttgacggcg atctccctgc
      541 gggtatgctc ctccgcgagg gagagacgcg cggcgagggt gcgcagctcc tgccggtact
      601 cggtgagcag gacttccttc tcctggagcg cttcctcggc ccgcttccag tcggtcatgt
      661 cgaggaagat caccgcgacg acggagcgag tggggctgaa ggcgatggcg tggaggtact
      721 tccccgtgtc gtagctgggt ccttcgtacc gggcgtagat cccggtggtg gccacctgga
      781 ggatgatgtc ctgccactcc cgctccctct ccgaccatat ctcgcggaag gggcggccga
      841 ggatgtcctc tttgcggatg cccatcacct gctcgtacgc gggattcacg tcgatgtaga
      901 ccacgtcgcg gaacgcgcct cgttcgtcga agagcgcccg gtagagggcc acgggttcca
      961 gtatttttgc gaagagggcg ccgagggcgt cgtatggggt ccggtcgtcg ttcatgccgc
     1021 tcactccttc ccggcttgtt ttctcccctc gattctacac gctgcggtga acatgaggca
     1081 actgctacca tgccgacgga ggttctccgc ccgtactccc cgcgcaacgc gcgtcgaacg
     1141 ccgcaaagag gcatgacaag cgggagcagc gcgctttgat tcctcattga ttcataaaat
     1201 cgtccaaagt tcagcctgag aattcataat ttgcgctacg ctacaatcat actttgcacg
     1261 atgcgcgccc ctcccccctt cccctagact actcaacgga ggaatatgct attccattcc
     1321 gggagggtga gcctatgaaa ctggagatag gaaagttttt tgtgaaggag atggtcttcg
     1381 gcgagaagac cggctacagc aagggcgtac tgaccataaa caaggaagag gctcttgcgg
     1441 tggtgaagga agacagccac atcaccgagg ctgagctgca catcgcatgc cccggagacg
     1501 aggtccgcat ggtccccgtg aaggaggcca tcgagatgcg ctgcaaggtc tccgggggcg
     1561 taatgttccc gggcgtgacc aaccccgtca tgcccgcagg gcagggaaga acgcacgcgc
     1621 tgaaggactg ctcgctgatc gtggtcggca agcactgggg gagcttccag gacggcctga
     1681 tcgacatggc cggccccttt cagaggaaca ccatcttcgg ctcgctgaag aatctcgtcc
     1741 tcgtgggcga cacggacgag gagttcgaac ggcacgagca gcagaagaag aaccgcgcgc
     1801 tccgctgggc cggacaccgt ctcgccgagt acgtcggcaa ggcagtcgcc gccctcgaac
     1861 cggaggagat cgagacatgg gagcttgagc cggtgaaccg ccgcgacgcc tccgtgcagg
     1921 gactcccttc cgtggtctac gtactgcagc cgcagacgca gatggaggct atggggtaca
     1981 acactctcgt ctacggctgg gacggcaacc acatgctccc caccttcatg caccccaacg
     2041 agctgctcga cggcgcgctg atctcaggca gcttcatgcc cgtctcgtcg aagatgtcca
     2101 cctacgagca ctgcaacaac cccgtcgtga agcgcctgat gaaggagcac ggcaagacgc
     2161 tcaacttcct cggcctcgtc atgtcgaacc tcaacgtcgc catggaccag aagcagcgct
     2221 ccgcctacat ggtggcgcag atggccaaaa acctcggcgc ggacggcgcg atcgtcgccg
     2281 aagaagggta cggcaacccc gacgtggact acatccagac catcgtcgaa ttggagaagg
     2341 tcggcatcaa gaccgtcggc atctcgaacg agtgcaccgg ccgcgacggc gggtcgcagc
     2401 ccctggtggc cctcgatccg gcggcgaacg ccctcgtctc caccggcaac gtctcgcagt
     2461 atttcgagct gcccccgatg aagaccgtcc tcggcgagct ggagtcgatg gcccgcg
//
LOCUS       DAOPKU010000445         2515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_47718_length_2515_cov_4.060976, whole genome shotgun sequence.
ACCESSION   DAOPKU010000445 DAOPKU010000000
VERSION     DAOPKU010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47718_length_2515_cov_4.060976"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..999)
                     /locus_tag="PK849_12010"
     CDS             complement(49..999)
                     /locus_tag="PK849_12010"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPE66896.1"
                     /translation="MGDKDRAFRRLLADERLFLKFLRRFLKQNLPDVIDVEALTLDDL
                     HREDITFIPPDLSERESDVLYRIRGTDAEAYVYVLVEHQSSVSHLMPFRLLSYVVQFW
                     TRYVEKVGEASKRKDFLLPPVLPVVFYEGATRWTAPVHFAEKVRNASDFGKFIPAFEY
                     RLISLRDKNPEALLSYGDALGALFYLANPSKTEGFLEAAERLRIFLLGLSDEERELTT
                     NHLRGYLRILMKKEGLEIDDALDLCLEREEADVMLTYMQKEFRKARKEGREEGREEGQ
                     LEKGILMALAMLEAGEPEEKILRYTGLSPSQLEEIRDGRK"
     gene            1284..1727
                     /locus_tag="PK849_12015"
     CDS             1284..1727
                     /locus_tag="PK849_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66897.1"
                     /translation="MDISRKRSALMVLIAFLWLGITALGMLKLFLPAMILGVVLMFVH
                     MFLGVARKGVVSKAFLVYPLLAWGVLWGASFILSKHYSDAFAGQMPAFTILGLHPSFA
                     WTVLTYWIGGMLTLTVGFMFLRSEWLSDKDWDEFLATIASGKEGA"
     gene            1730..>2515
                     /locus_tag="PK849_12020"
     CDS             1730..>2515
                     /locus_tag="PK849_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66898.1"
                     /translation="MEKTLIIGFSVAMIVASFLIGWWAKRKVTSNKAFFGSTGMFGPL
                     AVGLSSTAAVASAFALVGVPGIIYATGNPMTFWMLSSAAFTMAYLILGKKVRAMAEIG
                     EVASLGDISDLRFNNNRLIKAMLSIVIFLGCIAYLSAQIKAGSELFAHLLGWEPIVAA
                     IVIFGILTVYTAISGEVGGLLTQAFQGLVMVVAGVIMIVAFYTMTGGFGNVMQAVGSA
                     KEAAAAGVTKPLGPDFLNAWGRLPGTISMVWMLIPILGTIGQPQ"
BASE COUNT          466 a          795 c          687 g          567 t
ORIGIN      
        1 cggtctccga agggcttgct cgtcgagtcc tcctgtcttg gtcggcgatc actttctgcc
       61 gtcgcgtatc tcctccaact gcgatgggga aagaccggtg tagcggagaa tcttctcctc
      121 gggctcgccg gcttctagca tcgccagagc catcaagatt cccttttcca actgtccctc
      181 ttctcgcccc tcttctcgtc cttctttccg cgcctttcta aactctttct gcatgtaggt
      241 cagcatcacg tcagcctcct cccgttccag gcagaggtcc aaagcatcgt ctatctccag
      301 accttccttc ttcatcagga ttctcaagta ccctcgcaga tgatttgtcg tcagctcccg
      361 ctcttcgtcc gaaaggccaa gtagaaatat ccgcagccgc tccgccgcct ccagaaagcc
      421 ctccgtcttc gagggattcg ccaggtagaa gagcgcgccc agggcgtctc cgtaggagag
      481 aagcgcttcg gggttcttgt cgcgcagcga gatcagccga tactcgaagg ccggaatgaa
      541 ctttccgaag tcggaggcgt tccgcacctt ctccgcgaag tgcaccggcg cagtccacct
      601 ggttgcccct tcgtagaaaa ctaccggcag caccggaggc agtagaaaat ccttgcgctt
      661 cgaggcttca ccgaccttct cgacataccg tgtccaaaac tgaacaacgt acgaaagaag
      721 acggaatggc atcaggtggc tcacgctcga ctgatgctcc accagcacgt agacgtaggc
      781 ctcggcgtcg gtcccccgga tgcggtataa aacgtcggat tcccgctcgg agaggtccgg
      841 agggatgaac gtgatgtcct cgcgatgcag gtcgtcgagc gtgagcgctt ccacatcgat
      901 aacgtccggc aggttctgct tcaaaaacct tcggagaaac ttcagaaaga gccgctcgtc
      961 ggccagaagc ctgcgaaacg ccctgtcctt gtcgcccatg ggactcctct tttccacaaa
     1021 aagctttttg attattatag cacgcttgat ggagctgccg ctttgcgcgt gtttccggag
     1081 ttgttcatgt cgtgcaccgg cggaatttcc ggttccatag actgaaataa cagaaaaata
     1141 acggaaggtc cttgacaggc ttcggggagg agtgctattc tgtgcgcatt atgaagcatg
     1201 attcatgttt catttcgacg cgcccggtaa attgccggac ccgggccgtc gaggcgtcgc
     1261 acattcgaag gaggggatag gtaatggata tttccaggaa gagatccgca ctcatggtcc
     1321 tcatcgcgtt cctatggctc ggcatcaccg cgttgggcat gttgaagctg tttttgcccg
     1381 caatgatcct cggagttgtt cttatgttcg tgcacatgtt tctcggcgtg gcccgcaagg
     1441 gcgtcgtctc gaaggcgttt ctggtctacc cgctgctggc gtggggcgtg ctctggggcg
     1501 cgagcttcat cctctccaag cactattccg acgcgttcgc ggggcagatg ccggccttca
     1561 ccattctcgg ccttcacccc tctttcgcgt ggaccgtgct cacctactgg atcggtggta
     1621 tgctgacgct gacggtcggg ttcatgttcc tcaggagcga atggctgtcc gataaggact
     1681 gggacgagtt tctggcgaca atcgcaagcg gcaaggaggg tgcgtaacta tggaaaagac
     1741 actcatcatc gggttttccg tggcgatgat cgtcgcctcc ttcctcatcg gatggtgggc
     1801 gaagagaaag gtaacctcca ataaggcctt cttcggcagc accggcatgt tcggtccgct
     1861 cgcggtcgga ctctccagca ccgcggcggt ggccagcgcg ttcgctctgg tgggcgttcc
     1921 cggcatcatc tacgcgaccg gcaaccccat gaccttctgg atgctctcct ccgccgcctt
     1981 caccatggcc tacctgatcc tgggcaagaa agtccgtgcc atggccgaga tcggcgaggt
     2041 ggccagcctc ggcgacatat ccgacctccg cttcaacaac aaccggctca tcaaggccat
     2101 gctctccatc gtcatcttcc tcggctgcat cgcctatctc tccgcccaga tcaaggccgg
     2161 atcggaactc ttcgcccacc ttctcggctg ggagcccatc gtggccgcca tcgtcatctt
     2221 cggcatcctc accgtctaca ccgccatcag cggcgaagtg ggcggactcc tgacccaggc
     2281 tttccaggga cttgtcatgg tcgtggcggg cgtgatcatg atcgtcgcct tctacaccat
     2341 gaccggcggt ttcggaaacg tcatgcaggc cgtgggttcc gccaaggaag ccgccgcggc
     2401 cggcgtcacc aagcccctcg gccccgactt cctcaacgcc tggggcagac ttcccggcac
     2461 catcagcatg gtctggatgc tcatccccat cctcggcacc atcggccagc cccag
//
LOCUS       DAOPKU010000446         2506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_47982_length_2506_cov_2.889433, whole genome shotgun sequence.
ACCESSION   DAOPKU010000446 DAOPKU010000000
VERSION     DAOPKU010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47982_length_2506_cov_2.889433"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PK849_12025"
     CDS             complement(<1..549)
                     /locus_tag="PK849_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66899.1"
                     /translation="MGERRQGAGRRMAAAPSAETTPKGKRTRRMSGSGFQEEEMLDDV
                     SLDEGVDSVDPTDEEDDEFFEPSPLGSADGRARPIWLCAGDALLWCNPRFGYNIQFRE
                     TLRRLLETFLGNDGLRSMGKPFDSPDIEERLFLLLGFFREVWLRNLFKNEESDGKSLS
                     DEYAPPEEEDSGEESLSDEETPP"
     gene            complement(562..>2506)
                     /locus_tag="PK849_12030"
     CDS             complement(562..>2506)
                     /locus_tag="PK849_12030"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66900.1"
                     /translation="SDFGYRVLPGARDIRNRLSARCEDRFFFERLREWMEPAPLAEAA
                     CALDGMLRTEPVWCLMIRQPSLHRHSVQAFRVRIWEKSVIGMPPLSTAGFNADFDGDT
                     MAVFLPPEPYAQDLSRFSLLENPGIVGTGAPAFATGLDLALGWNALSSEEKAVFFEEA
                     GCPFDPSMSFKKFFSKLLRALGKHPVDERRASLRTLQKEVCRASTGQASLGPAVFQRL
                     CDAFRARFQDDIHGFAKCTDEEYDSIISRVNEALKSGMSGAISLLVGSGAKGGPDDLR
                     AMGAFLGAQKLFRNDEKDSPEEVARRGFIGSNLWEGLSEEEMFVYSYASRDSMASKKL
                     AVAEAGYLSRLLAEGLFETFVTDEDCGATKGLSIAFGKDAMLSLAFPGSRTFTPLPTM
                     GSVVDDLSRVAWGRVPVGMSRCLNERDLREIAGYWRGEADVSDEVLRTHLAERGGVLE
                     IRSPLSCSAVSPCVCSLCCGADLPRKPFDDPAPLPLGARVGLTAAQAIGERGTQLAMK
                     RFHQVGDPKNTAKNDAASDGEETTLPGGVPAVLRALLVDAGADSEKTQEERFVSLLEN
                     VLASGKGEKEKKKAEKDLPQSLIHFEIALRPEKGLYSEAESRIGNAFLSRLAFEGAGE
                     VLKEIDKECIDDLTSTKSRLLWS"
BASE COUNT          549 a          833 c          610 g          514 t
ORIGIN      
        1 cggaggagtc tcttcatcgg aaaggctttc ctcgccggaa tcctcttctt ccggaggggc
       61 gtattcgtca gaaaggcttt tcccgtcgga ttcctcgttt ttgaaaagat ttctcagcca
      121 cacctcccgg aaaaagccca aaagtagaaa aagcctttcc tcgatatccg gggaatcgaa
      181 cggcttcccc atgctccgca ggccgtcgtt tcctaaaaat gtttccagaa gccttcgcaa
      241 tgtttcccga aactggatat tataaccgaa gcgcggattg caccatagaa gagcatctcc
      301 ggcgcacagc cagatcggcc tcgctctccc gtcggcggaa ccgagaggag acggttcaaa
      361 aaactcgtcg tcctcttcat ccgtgggatc aacactatct actccttcgt cgagagaaac
      421 gtcatcaagc atttcctctt cctgaaagcc gctaccgctc attctccgcg ttcttttccc
      481 cttcggcgtc gtttccgccg aaggggctgc ggccattctt cttcccgctc cctgtctgcg
      541 ttcacccaac gacagcaccc cctaactcca gagcagacga ctctttgtgc tcgtaagatc
      601 gtcgatacac tctttgtcga tctccttcag cacttcgccc gctccttcaa aagccaatct
      661 ggagagaaac gcgttcccga tgcggctctc ggcttcgctg taaagcccct tttccggccg
      721 cagcgcaatc tcgaagtgaa taaggctctg cggaagatcc ttctccgcct tctttttttc
      781 cttttcgcct tttccggagg ccagcacatt ttctaacaag gaaacgaagc gttcttcctg
      841 agttttttcg gaatcggcgc cggcatccac taaaagagcg cgcaaaacag ccgggacgcc
      901 gccggggagc gtcgtttctt ccccgtcact tgccgcatca ttcttcgccg tattcttcgg
      961 gtcgcccacc tgatggaagc gcttcatcgc aagctgcgtt cctcgttccc ctatcgcttg
     1021 tgccgcagtc agtcccactc gtgcgccgag cggcagagga gccgggtcgt cgaagggctt
     1081 ccttgggaga tccgctccgc aacacaaaga gcacacacac ggggatacag cgctgcagga
     1141 aagaggagaa cgaatttcca gcacgccgcc gcgttccgcg agatgagtcc gcaacacctc
     1201 gtcggaaaca tccgcctcgc ccctccaata ccctgcgatc tcccgaagat cccgctcgtt
     1261 caaacagcgc gacatcccga cagggacccg cccccaggcg accctgctca gatcgtcgac
     1321 tacgctcccc atcgtaggca gaggcgtaaa agtccggctc ccggggaacg ccaaggaaag
     1381 catcgcgtcc ttgccgaacg caatagagag gcctttggtc gcgccgcaat cttcgtccgt
     1441 cacaaaggtc tcgaacaagc cctccgcgag cagccgcgaa agataccccg cctccgcaac
     1501 ggcaagcttc ttcgaggcca tggaatccct tgaagcatac gaatacacaa acatctcttc
     1561 ctctgaaagc ccctcccaga ggttcgaccc gataaagcct ctccgtgcaa cctcctcggg
     1621 agaatctttc tcatcgttcc gaaagagctt ctgagccccc agaaacgccc ccatggctcg
     1681 cagatcgtcg ggtcctccct tggcgccgct gccgacaaga agagagatcg ccccggacat
     1741 accgcttttc agcgcttcgt tcacccgcga aatgatgctg tcgtattctt cgtcggtgca
     1801 cttcgcaaac ccgtggatat catcttgaaa gcgtgcgcgg aaagcgtcgc acagccgctg
     1861 aaagaccgcc ggaccaaggc tcgcctgccc cgtcgatgcc cgacagacct ctttctgaag
     1921 cgttcgaagc gacgctctcc gctcgtcgac aggatgtttt ccaagcgcac gaagaagctt
     1981 cgagaaaaat ttcttgaacg acattgacgg atcgaacgga cacccggcct cttcaaaaaa
     2041 gacggccttc tcctcactcg acagagcatt ccagcccaac gccaggtcaa gtcctgtagc
     2101 gaacgcgggc gctcccgttc caacgattcc ggggttttcc aaaagggaaa accgcgacaa
     2161 atcctgcgca tacggctccg gcggcagaaa aacagccatt gtatccccgt cgaagtccgc
     2221 gttgaagccc gcagtcgaaa gaggcggcat cccgatcacg cttttctccc atatccggac
     2281 gcgaaaagcc tgcacgctgt gtctgtgcag gctcggctgc cgtatcataa gacaccaaac
     2341 aggctccgtt cgcagcattc cgtcgagagc gcacgcagcc tcggcgagcg gcgcaggctc
     2401 catccactcc cgcagacgtt cgaagaaaaa acggtcctcg cagcgtgccg acaagcgatt
     2461 tcggatatcg cgtgctcccg ggagcacgcg atatccgaaa tcgctt
//
LOCUS       DAOPKU010000447         2504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48034_length_2504_cov_3.531646, whole genome shotgun sequence.
ACCESSION   DAOPKU010000447 DAOPKU010000000
VERSION     DAOPKU010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48034_length_2504_cov_3.531646"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /gene="glgP"
                     /locus_tag="PK849_12035"
     CDS             complement(<1..1678)
                     /gene="glgP"
                     /locus_tag="PK849_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPE66901.1"
                     /translation="MMSLHHQRELGSSIRKLMETDPAFRPVAYLSMEIGIKESLPTYS
                     GGLGILAGDILKSAADLGVPMVALTLLYRKGYFQQSFNEDGWQTEKPVLWQPVQELTL
                     LPNQVSLTLQNREVHVRVWQYEIVGNTGHPLPVYFLDTDFDNNHPDDRKLSWQLYGGD
                     QLYRLCQEMVLGVGGLRMLRDLGYKNIETFHMNEGHAGFLTLELMREQGYFDIEKIRE
                     QVIFTTHTPVPAGHDFFRFDLVDKVISQEALSNLKRMLPNSDGVSMTELGIRFSRYVN
                     AVSKKHAEVSRNMFTMPEIDWVTNGVHPGTWVSTGMRRLFNTHIPGWENDPGRLVQAV
                     KIPPEDIWSAHQASKLRLLAGIMEQTGQQFEPDILTIGFARRAATYKRADLLFSNMKR
                     LLEVTSGKIQLVFAGKAHPKDDGGKAVLQRLLSASQKVGEMIPIVFLENYDMEKGAMI
                     TQGVDLWLNTPLRPREASGTSGMKCTLNGVMNFSVLDGWWIEGWIEDVTGWSIGPDPT
                     ESEMVHYDESLDANDLYDKLENKIIPTYYNNREKWIWMMQRTIAMNGSFFN"
     gene            complement(1865..>2504)
                     /locus_tag="PK849_12040"
     CDS             complement(1865..>2504)
                     /locus_tag="PK849_12040"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPE66902.1"
                     /translation="LPTAHLSDAFLRGLLAYWAVPFGDRTAEKGVWRDVPSEAFFASL
                     RKNIPSASFLAENLGVITPDVTDAMERMSFPGMAVVLFAFGGGMKDNPHIPHNYSATL
                     AAYSGTHDNNTALGWYRDDASEEERNTLGRYLGRMPDEKEVPDIIVRMVLSSVAQRAI
                     IPLQDYLGLGSEARMNTPATPSGNWEWRADELCFTEELSIRIKQLSEIYGRN"
BASE COUNT          493 a          685 c          730 g          596 t
ORIGIN      
        1 tattgaaaaa gctcccgttc atggcgatgg tccgctgcat catccagatc catttttcgc
       61 ggttgttgta gtaggtcgga atgattttgt tctccagctt gtcatagagg tcgtttgcgt
      121 ccagcgattc gtcgtagtgg accatctccg actccgtggg atcggggcct atcgaccatc
      181 cggtgacgtc ctcgatccac ccttctatcc accatccgtc gaggacggaa aagttcatga
      241 ccccgttcag cgtgcacttc atgccgcttg ttccggacgc ttcccgcggg cggagcgggg
      301 tattcagcca aagatcgacg ccctgtgtga tcatcgctcc cttttccatg tcgtagttct
      361 caaggaagac gatggggatc atctctccaa ccttctggga agcgctcaga agacgctgga
      421 gaacagcctt gcctccgtcg tccttcgggt gcgctttccc tgcgaagacg agctgtattt
      481 tgcctgaggt cacttcaaga aggcgtttca tattggaaaa aagaagatcc gcacgtttgt
      541 acgtcgcggc tctccgtgca aagcctatgg tcagaatatc cggttcgaac tgctgtccgg
      601 tttgttccat gattccggcg aggaggcgca gtttcgacgc ctggtgcgcg ctccagatat
      661 cctcgggagg gatcttcact gcctggacta accgtccggg atcattctcc cagcccggaa
      721 tatgcgtgtt gaagagcctg cgcataccag tgctcaccca tgtgcccgga tgcacgccgt
      781 tggtgaccca gtctatctcc ggcatggtga acatgttccg gctgacctcg gcgtgttttt
      841 tcgagacggc gttgacgtag cggctgaaac ggatgcccag ttcggtcatg gaaacgccgt
      901 ccgagttcgg gagcatcctc ttgagattgg agagcgcctc ttgcgagatg accttgtcca
      961 caaggtcgaa acggaagaag tcgtgccctg ccggaaccgg ggtatgcgtc gtgaagatga
     1021 cctgttcacg gatcttttcg atgtcgaagt agccttgttc cctcatgagt tcgagggtga
     1081 ggaaaccggc gtgtccctcg ttcatgtgga acgtctcgat gtttttgtac ccgaggtcgc
     1141 ggagcatgcg gagaccgccc acgccgagca ccatctcctg gcagaggcgg tagagctggt
     1201 cccctccgta gagctgccac gagagtttgc ggtcgtcggg gtgattgttg tcgaaatcgg
     1261 tgtccaggaa gtagaccggg agcggatgtc ccgtgttgcc gacgatctcg tactgccata
     1321 cccgtacgtg gacctcgcgg ttttgcagcg tgagcgacac ctggttgggc agaagcgtca
     1381 actcctgcac cggctgccag agcacgggtt tttccgtctg ccagccgtcc tcgttgaacg
     1441 actgctggaa ataccctttc ctgtaaagaa gcgtgagggc gaccatggga acgccaaggt
     1501 cggccgcgct tttcaggatg tctccggcaa ggatgcccag acctcccgaa taggtcggga
     1561 gagattcttt tatgccgatc tccatggaaa gatatgctac cggacgaaac gccgggtcgg
     1621 tttccattag tttgcggatt gaactgccaa gctctctctg atggtgcagt gacatcatgg
     1681 gagcgactcc tcctctcaag tagtacagat tcaaacgttg gggctttcgt gttgcgaccg
     1741 gagaagaaac gctctggatt tatcttgcgt atcatccgcc ggaagccgtg cccgaaaaag
     1801 gaacccacga agcctttgat tcataatagt accagaaaaa gccctcttct ttgaaggggg
     1861 aacatcaatt tcttccgtaa atttcggaga gttgctttat tctgattgaa agttcttcgg
     1921 tgaagcacaa ttcgtcagcg cgccactccc agttccccga gggggtcgcg ggcgtgttca
     1981 tgcgcgcttc gctcccaagt cccaggtagt cctggagcgg gatgattgcg cgctgtgcaa
     2041 ccgaggagag taccatcctt acgataatgt ccggaacctc cttctcgtcc ggcattcggc
     2101 cgagatagcg ccccagcgtg ttccgttctt cctccgaggc gtcgtcgcgg taccagccga
     2161 gcgcggtgtt gttgtcgtgt gttccgctgt aggctgcaag cgttgcggag tagttgtgcg
     2221 gaatgtgcgg attgtccttc atcccgcctc cgaacgcaaa aagaacgact gccattccgg
     2281 gaaagctcat tcgttccatg gcgtccgtga catcgggtgt aatgacgccc aggttttcgg
     2341 ccaggaatga agcggagggg atgttctttc ggagtgaagc aaagaacgct tccgacggca
     2401 catctctcca gacgcctttc tcagcggtcc tgtcgccgaa agggactgcc cagtaggcaa
     2461 ggagtccgcg gagaaaggcg tcagagagat gtgccgtcgg aagc
//
LOCUS       DAOPKU010000448         2499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48177_length_2499_cov_4.137889, whole genome shotgun sequence.
ACCESSION   DAOPKU010000448 DAOPKU010000000
VERSION     DAOPKU010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48177_length_2499_cov_4.137889"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PK849_12045"
     CDS             <1..459
                     /locus_tag="PK849_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HPE66903.1"
                     /translation="FTSPCSEDIPLMDSNPGEVRSRAYDCVLNGNELGGGSIRIHDPL
                     LQEKVFSCLSFTKEAARERFGFLLDALSSGTPPHGGIALGMDRLAMLLSGAASIRDVM
                     AFPKTQKAQCLMSGAPSPVPEEQLKELCIKTAPSAPSEAPAPAQEKMDLK"
     gene            520..1206
                     /locus_tag="PK849_12050"
     CDS             520..1206
                     /locus_tag="PK849_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPE66904.1"
                     /translation="MATLRYLGHSAFEIRGKDTVLLIDPFLSGNPAGAAATESEFTDV
                     RYIFVTHGHGDHLGDTVSIASRTGATVVTNYEICHYLGKKGVTCHAMHIGGTFSFPFG
                     RVKMTPALHGSDIMEGDRMIPGGNPCGFLFDVDGDRIYHAGDTGLTMDMQLLAVEKVK
                     AALIPIGGNFVMDVYDAARAVEFVRPEIAVPIHYDTFPVIAADPREFERLVGANATVK
                     IMKTGDTLEL"
     gene            complement(1333..2199)
                     /locus_tag="PK849_12055"
     CDS             complement(1333..2199)
                     /locus_tag="PK849_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPE66905.1"
                     /translation="MDSSQLQALLRSKVETMPNKARRVVEYLLANAREAAFLSIGEVA
                     EKLNVSKAQLVRVSRMVGFDGYAALKEALQDTVLEQVNPAALLGRIMKNRQDLPEEIL
                     RLEHANLDDTWNQLQPERIVAFCEMIKEARMVYCMGWGISSIVAEALFTRLLEMGIPS
                     ALMKRGSLALIEQARTVTTGDVVVVCELPSYVIEVTESVQKAASNGASIISLTDSPAA
                     PVCQSSTLSFFVSDTTPTFGSSLVGPAFLVHILTSVLAVNLGESARVALQDQEMGLHD
                     ERIYHPAYGLRY"
BASE COUNT          549 a          652 c          695 g          603 t
ORIGIN      
        1 ttcacctctc cctgctctga agatattccg ctcatggatt caaatcccgg cgaggtgcgg
       61 tccagggcgt acgactgcgt cctcaacggc aacgagcttg gcggcggttc catcagaatc
      121 catgacccgc ttctgcagga aaaggttttc tcctgtctct cctttacaaa agaagcggcg
      181 cgagagcgtt tcggattcct gctcgacgcg ctttcttccg gcactcctcc ccacggaggt
      241 atcgctcttg gaatggacag gctggcgatg ctcctgagcg gcgctgcatc cattcgcgac
      301 gtgatggcgt tcccaaagac gcaaaaggct caatgtctta tgtctggcgc gccgtcgcca
      361 gtaccggagg agcagttgaa agaactctgc ataaagacag ctccgtcggc gccgtctgaa
      421 gcgccagctc cggcgcagga gaaaatggac ctgaagtgat cacgaaatcc gggcccccgt
      481 gttcacagcg ggggcccgtg cataaaggag gagatatatt tggcgacatt acggtatctc
      541 ggacattcgg cattcgagat tcgggggaag gatacggtgc ttctgataga tccgtttctt
      601 tcgggaaatc ctgcgggcgc cgcggcgacc gagagtgagt tcacggatgt acgctatatc
      661 ttcgttaccc acggacacgg agatcatctg ggcgatactg tctccatcgc ttccaggaca
      721 ggcgccacgg ttgtaacgaa ttacgaaata tgtcattacc tcggaaaaaa gggcgttacg
      781 tgccacgcaa tgcatatcgg tggtacattc tccttcccct tcggacgcgt caagatgact
      841 cctgcgctcc acgggtcgga tatcatggag ggggaccgga tgatacctgg gggaaatccc
      901 tgcggttttc tctttgacgt ggacggagat cggatctatc atgcaggaga caccggtctt
      961 acgatggata tgcagcttct ggctgtagaa aaggtaaagg cggcgctgat ccccatcggc
     1021 gggaactttg tgatggacgt gtacgacgcc gcccgtgccg tggagtttgt ccgccctgaa
     1081 atagctgtgc cgatacacta tgacactttt ccggtgatcg cggcggatcc gcgtgagttt
     1141 gagcgccttg tcggtgcgaa cgcgactgtg aagatcatga aaaccgggga tacgctggag
     1201 ctgtgataag aagggtaaac ggatgcaacg cggtcaaaga gaataatgaa ttaccgccgg
     1261 tcttctttag acaggaatta tgatagtgtt cctgctcaaa gaggaccgga agtatgcagg
     1321 gcgcaggggg tttcagtatc tcaatccgta tgcggggtgg taaatgcgtt cgtcgtgaag
     1381 tcccatctcc tgatcttgca gcgctacacg ggcggattcg cccaggttga cggcaagaac
     1441 agaagtaagg atgtgtacga ggaaggccgg accgaccaga ctgctgccga aggttggcgt
     1501 cgtatcgctc acgaagaagg agagcgtcga cgactggcag acaggggcgg cgggactgtc
     1561 agtgagcgag attattgatg cgccgttcga cgcagctttt tgaacggact ctgtgacttc
     1621 gatgacgtag ctcggcagtt cgcagacgac gacgacatcg cctgttgtca ctgtccttgc
     1681 ctgttcaata agcgcgagag agcctctctt catcaaggcg cttgggatgc ccatttccag
     1741 cagacgggtg aaaagcgctt ccgcgacgat cgacgagatt ccccacccca tgcaatacac
     1801 cattctcgct tccttgatca tctcgcaaaa cgcgacgatt ctttcaggtt ggagctgatt
     1861 ccacgtatcg tcaagattgg cgtgttccag ccgcagaatc tcctcgggga gatcctgtct
     1921 gtttttcata atgcgtccga gaagcgccgc aggattcacc tgttcgagca ccgtatcctg
     1981 gagggcttcc ttcaatgccg cgtagccgtc gaaaccaacc atgcgcgaca cgcgtaccag
     2041 ttgtgctttc gagacgttca gcttttccgc aacttcgccg attgagagaa acgccgcctc
     2101 cctggcgttt gccagaaggt attcgacaac ccttctcgcc ttgtttggca ttgtttccac
     2161 tttgcttcgc aacagtgcct ggagttgcga actatccatg aaagacacct ccgaaactgt
     2221 ttagtacttc gaattttctc ttgagactta agtttattct gttggagatg cgccgtcaat
     2281 cgttctccgc tcattttctt gttttttcga tagaaaatta tttaagatcg tatatagcgt
     2341 atttgttttt ctatgaactg caagcgaaag cctgtttttt gcgccatgta gcgcagtccg
     2401 aatttatcgt gacctgtcag aagcgatatc tgccttctct ctacttccca tggtcccgtc
     2461 gaagaacttt ccagaaggga ggcgacaaag gcgactgag
//
LOCUS       DAOPKU010000449         2497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48227_length_2497_cov_5.423014, whole genome shotgun sequence.
ACCESSION   DAOPKU010000449 DAOPKU010000000
VERSION     DAOPKU010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48227_length_2497_cov_5.423014"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PK849_12060"
     CDS             <1..629
                     /locus_tag="PK849_12060"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPE66906.1"
                     /translation="AWNAGRFDEEIVPVPVTVKKQTVLFDRDEHPRAGVSSGELAALK
                     PAFKKEGGTVTAGNASGINDGAAAVVVMSEQKAKELGVVPMAVFEGGASAGVDPCVMG
                     IGPAFSTRKLLERTGRGIAEFDLIEANEAFAAQALAVGKLLEWDWNRVNVNGGAIALG
                     HPVGASGCRILVTLLHEMRRRGVRNGLATLCVGGGMGVSTAVGLYDGR"
     gene            647..1366
                     /gene="fabG"
                     /locus_tag="PK849_12065"
     CDS             647..1366
                     /gene="fabG"
                     /locus_tag="PK849_12065"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPE66907.1"
                     /translation="MRLENKTAIVTGGARGIGQAMCELFASEGARVLAADMSELSYEN
                     PSVEGYRLDVSDPEACRACFEYAVGRYGKIDVLVNNAGITRDALTRKMTDEMWHKVIG
                     VNLSGVFNLGRLVGPHMQEHGGGSIVCISSVVGEFGNIGQANYAAAKAGMIGLAKTWA
                     KEFAMKGGNVRVNVIAPGYTMTDILSTVPQELLDKFAAMTMLGRLGRPEEIAKAALFL
                     ASDDASYVTGHVLSVNGGMRL"
     gene            1454..>2497
                     /locus_tag="PK849_12070"
     CDS             1454..>2497
                     /locus_tag="PK849_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPE66908.1"
                     /translation="MNFELSKEHRMARDLFRRFAEKEARPLAQEVDENERFPSETVEK
                     MARSGFLGIPCGKEWGGQGCDTLTYVLCVEELSRVCATTGVVVSAHTSLACDPIEKYG
                     TEAQKRKYLPELLAGRKLGAFALTEPGAGTDASGQQTRAVLEGENYRLNGSKIFITNA
                     GQADVYIVFAMTDKSLGTKGISAFIVEKGFPGFSVGKKELKMGIRGSSTCELIFEDCV
                     VPKENLLGKEGKGFSIAMGTLDGGRIGIAAQALGIAQGALDETVSYVKERKQFGKPIG
                     AFQNTQFRMADMKTRIEAARLLVYGAALAKDSGGRFSVEAAMAKLFASETAMEVTTKC
                     VQLYGGYGYMRDYP"
BASE COUNT          478 a          739 c          896 g          384 t
ORIGIN      
        1 cggcgtggaa tgccgggcgg ttcgacgagg agatcgtccc cgttccggtg acggtcaaga
       61 agcagaccgt tctcttcgat cgcgacgaac accctcgcgc gggcgtctcc tccggggagc
      121 tggcggcgct gaaacccgcc ttcaaaaagg agggggggac ggtgaccgcg gggaacgcct
      181 ccggcatcaa cgacggggcg gctgcggtcg tcgtcatgag cgagcaaaaa gcgaaggagc
      241 tgggcgtcgt gccgatggcc gtcttcgagg gcggcgcgtc ggcgggggtc gacccctgcg
      301 tcatggggat aggccccgcg ttcagcacgc gaaagctgct ggagcggacg gggcggggca
      361 tcgcggagtt cgacctgatc gaggcgaacg aggcgttcgc ggcccaggcg ctcgccgtag
      421 gaaagctgct ggagtgggac tggaaccgcg tgaacgtgaa cggaggggcc atcgccctcg
      481 gacaccccgt gggcgcttcc ggctgccgga tccttgtgac gctgctccac gagatgcggc
      541 gaagaggcgt ccgaaacggg ctcgccacgc tctgcgtcgg cggcgggatg ggcgtctcga
      601 cggccgttgg actttacgac ggccggtgat ggaaggagat gtgcgaatga gacttgagaa
      661 taaaaccgcc atcgtcaccg gcggggcgcg cggcatcggg caggcgatgt gcgaactgtt
      721 cgcctcggag ggcgcgcgcg tcctcgcggc ggacatgtcg gagctgtcgt atgagaatcc
      781 gtcggtcgag gggtacaggc tcgacgtctc cgacccggaa gcgtgcaggg cgtgcttcga
      841 gtacgcggtc gggcggtacg gcaaaatcga cgtcctcgtg aacaacgcgg gaatcacccg
      901 cgacgcgctg acgcggaaga tgaccgacga gatgtggcac aaggtcatcg gcgtcaacct
      961 gagcggcgtc ttcaacctcg gacggctcgt cgggccgcac atgcaggagc acgggggcgg
     1021 ctccatcgtc tgcatctcct ccgtcgtggg ggagttcggc aacatcggac aggcgaacta
     1081 cgccgcagcc aaggccggaa tgatcggcct ggcgaagacc tgggccaagg agttcgcgat
     1141 gaagggcggt aacgtccgcg tgaacgtgat cgctccgggg tacaccatga ccgacattct
     1201 gagcaccgtg ccgcaggagc tgctggacaa gttcgccgcc atgaccatgc tcgggcgcct
     1261 cgggcggcct gaggagatcg cgaaggcggc gctcttcctg gcgagcgacg acgcatccta
     1321 cgtcaccggg catgtgctca gcgtgaacgg cggcatgcgg ctgtaacgaa cgaatctatt
     1381 caaccacccg ccccgcgccg ggcccgttcg cggagcctcg gcgcggggcg cggcactttc
     1441 ggaggcgtac cggatgaact ttgaactgag caaggaacat cgaatggcga gagacctctt
     1501 ccgccgtttc gcggagaagg aagcgcggcc tctggcgcag gaagtggacg aaaacgaacg
     1561 cttcccgtcg gagacggtgg agaagatggc gcgctccggt tttttgggta ttccctgcgg
     1621 caaagagtgg ggaggacagg ggtgcgacac gctgacctac gtcctctgcg tggaggagct
     1681 gagccgagtc tgcgcgacca ctggcgtcgt cgtctccgcc cacacctcgc tggcctgcga
     1741 cccgatagaa aagtacggca cggaggcgca aaaacggaag tacctcccgg agctcctggc
     1801 ggggcggaag ctgggcgcgt tcgcgctcac cgagcccggg gccggtacgg acgcctcggg
     1861 gcagcagacc agggcggttc ttgaaggaga gaactaccgg ctgaacggca gcaagatctt
     1921 cattacgaac gccgggcagg cggatgtgta tatagtcttt gcgatgaccg acaaaagcct
     1981 cggcacgaag ggcatctccg ccttcatcgt ggagaagggc tttcccggat tttcagtcgg
     2041 gaagaaggaa ttgaagatgg gaatccgcgg ctcctcgacg tgcgaactca tcttcgagga
     2101 ctgcgtcgtg ccgaaggaga atctgcttgg caaagagggg aagggcttct ccatcgcgat
     2161 ggggacgctc gacggcgggc gcatcggcat cgcggcccag gcgctcggca tcgcgcaggg
     2221 agcgctcgac gagaccgtct cctacgtcaa ggaacgaaaa cagttcggca agccgatcgg
     2281 cgccttccag aacacacagt tccggatggc cgacatgaag acgcggatcg aggccgccag
     2341 actgctggtt tacggcgcgg cgctcgccaa ggactccggc ggacgattct ccgtcgaggc
     2401 ggcgatggcg aagctcttcg cctccgagac cgcgatggaa gtgaccacga agtgcgtgca
     2461 gctctacggc ggctacggct acatgcgcga ctacccc
//
LOCUS       DAOPKU010000450         2495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48304_length_2495_cov_3.273770, whole genome shotgun sequence.
ACCESSION   DAOPKU010000450 DAOPKU010000000
VERSION     DAOPKU010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48304_length_2495_cov_3.273770"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PK849_12075"
     CDS             <1..834
                     /locus_tag="PK849_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66909.1"
                     /translation="HDPLVPGAVWEDRSWKAARNVVVFSENTPYELPGATLYPCPLRG
                     KQSDADPTSWIRAEGGDRIHIGLAHGSVEGAPIGEWDHPIPRDAASRSGLDYLALGHW
                     HSFGLFGTKDAPRTAYSGTHEPTSFGERDSGNALIVDIEAPGCAPVLTPVRTGGISWI
                     SIEEQVDSRDALESVRERIGTLQNPERTLLRVRLSGLMRPDDRDALEEIALAAASFLW
                     SEVDESGLLPSPEDDSWIGNLPAGLLREVGKRLNQAQEAPEIRTKALLELYALLSEVK
                     A"
     gene            831..>2495
                     /locus_tag="PK849_12080"
     CDS             831..>2495
                     /locus_tag="PK849_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE66910.1"
                     /translation="MILHTLVVENWRCILGERSVGPFSEGVNILHAPNATGKSTLFEA
                     LRCALMDNHNTKGEEIQKLRPWGRSLSPRVVVEFSTGGVRYRVRKGFLDTPESLLERL
                     EKGTYQPVAKGPEANKTTRALFTKDAPKKGMSRPEHWGVQQLLWAPQGRLELGELSGN
                     LLDDIRTSITAQVVDDRARGVEELLKKRYETYFTPTGKVSKNSALTQYEQAIRETAAE
                     LAERRMEHAEAESLSKRVRALSEKAAALRGETGRMEAETNALEEKAAAYEKMRAEFEL
                     RSAGAHAAEAGYGRLKTAVEQIDDAIKERDTVKKRLEELRSALPQLEAERKECVKVLE
                     AAQLALEAAREETKTAEERANQARAAHRAVELKEQRHALIEKGAAVQATQSLLSEHEK
                     EKASGHFPSLAEIKKIRKAFADLEKATIQLDASRISLEITPARALSGRIDAGDDQGPL
                     TLEAGKPTALHGAPEIRLEIESFGVLRAFGPCESAQEALEDARKAEAHVQKLSAPFGT
                     NDRTDLEDLHDRGSSLDSHIERERAELNALLGKETAETLSARLKQID"
BASE COUNT          527 a          806 c          813 g          349 t
ORIGIN      
        1 cacgaccccc tcgtcccggg agccgtctgg gaggaccgtt cctggaaggc ggcccgcaat
       61 gtcgtcgtct tcagtgaaaa cactccgtac gagcttccgg gcgcgaccct ctacccctgc
      121 ccgctgcggg gaaagcagtc cgacgcggac ccgacctcgt ggatacgggc ggaagggggc
      181 gaccggatcc atatcggact ggcccacggc tcagtcgaag gcgcccccat cggcgagtgg
      241 gaccacccga tcccgagaga tgcggcgtcg cgctccggac tcgactacct tgcgctcggc
      301 cactggcact ccttcggact gttcggaaca aaggacgccc cgaggacggc ctattccgga
      361 acgcacgaac cgacctcgtt cggcgagcgg gacagcggca acgcgctcat cgtcgacatc
      421 gaagcgcccg gatgcgcccc tgtcctcacg cccgtccgca cggggggcat ctcctggata
      481 tccatcgagg agcaggtcga ctcccgggat gccctcgaaa gcgtccgcga acgaatcggc
      541 acgcttcaaa acccggagcg tacccttctc cgcgtccgcc tttccggact gatgcggccg
      601 gacgaccgcg acgcgcttga ggagattgcg ctggcggcgg cgtcttttct ctggagcgaa
      661 gtggacgaga gcggactcct gccctcgccc gaagacgact cctggatcgg caacctgccc
      721 gcggggctgc ttcgggaggt gggcaaacgc ctgaaccagg cgcaggaagc gccggaaatc
      781 aggacgaagg ctcttctcga actctatgcc cttctatcgg aggtgaaggc atgattctgc
      841 acacactcgt tgtcgagaac tggcgatgca ttctgggaga gcgctccgtc gggcccttct
      901 ccgagggggt gaacatcctc cacgccccca acgcgacggg gaaatcgacg ctcttcgaag
      961 cgctccgctg cgccctgatg gacaaccaca atacgaaggg ggaagagatt caaaaactcc
     1021 gcccctgggg gcgctcgctc tccccccgcg tcgtggtcga gttcagcaca ggcggcgtgc
     1081 ggtaccgggt ccggaagggg tttctggata cgccggaatc actcctcgaa cggctcgaaa
     1141 agggaacgta ccaacccgtc gccaaggggc ccgaggcgaa taaaacgacg cgcgcgctct
     1201 tcacgaagga tgcaccgaag aagggcatgt cgcgcccgga gcactgggga gtccagcagc
     1261 tcctgtgggc gccgcagggc aggctcgaac tcggcgaact gtccggcaat ctcctcgacg
     1321 acatccggac gtccatcacc gcccaggtcg tcgacgaccg cgccaggggc gtggaagagc
     1381 tgttgaagaa acggtacgag acctacttca ccccgacggg gaaggttagt aaaaacagcg
     1441 cgcttacgca gtacgagcag gcgatccggg agacggccgc ggaactggcg gagcgcagga
     1501 tggagcatgc ggaggcggaa tcgttgtcga aacgcgtccg ggccctctcg gaaaaggcgg
     1561 cggccctccg gggcgaaacc ggacgcatgg aagcggagac gaacgcgctc gaagagaaag
     1621 ccgcggcgta tgaaaaaatg cgggccgagt tcgaacttcg ctccgccggc gcacacgccg
     1681 ccgaggcggg gtacggacgg ctgaagactg ccgtggagca gatcgacgac gcgatcaaag
     1741 aaagagacac cgtcaaaaaa cggctcgaag aactccgctc cgcgctgccg cagcttgaag
     1801 cggagcgtaa agagtgcgta aaggtcctcg aagcggccca gctcgcgctc gaagcggcac
     1861 gggaagagac caagactgcg gaggagcgcg cgaaccaagc ccgcgcggcg cacagagccg
     1921 tcgaactcaa ggagcagcgt cacgcgctca tcgagaaagg cgccgctgtg caggcgacgc
     1981 agtcactcct ctccgaacat gagaaggaaa aggcctccgg gcacttcccc tcgcttgcgg
     2041 agatcaaaaa gatccgcaag gcgttcgcgg acctcgaaaa agcgacgatt cagctcgacg
     2101 cctcgcgtat ttctcttgaa atcacccccg cgagggcgct ctcgggccgc atcgacgcgg
     2161 gagacgatca gggccccctg acgctggagg ccggaaagcc aacggcgctc cacggcgccc
     2221 ccgaaatacg cctcgaaatc gaatccttcg gcgtgctgcg cgccttcggc ccctgcgagt
     2281 ccgctcagga ggcgctcgaa gatgcgcgga aggcggaggc gcatgttcaa aagctttccg
     2341 ccccgttcgg gacgaacgac aggacggatc tcgaagattt gcacgaccgg gggagttcgc
     2401 tcgactccca tatcgaacgc gagcgagcgg agctgaacgc ccttctgggc aaggagaccg
     2461 ccgaaacgtt gtcggcgcgg ctgaagcaga tcgac
//
LOCUS       DAOPKU010000451         2491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48415_length_2491_cov_3.980296, whole genome shotgun sequence.
ACCESSION   DAOPKU010000451 DAOPKU010000000
VERSION     DAOPKU010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48415_length_2491_cov_3.980296"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PK849_12085"
     CDS             <1..316
                     /locus_tag="PK849_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HPE66911.1"
                     /translation="VQDFIPTPGSLSTCMYYTGLNPFTGEAVPSAKERNERKMQRVLL
                     QYAEPKNREAVIQALEAAGRKDLIGTGPSCLVQPQHREPQKKSAPRPKGGTRSGKPDG
                     RR"
     gene            complement(318..1229)
                     /locus_tag="PK849_12090"
     CDS             complement(318..1229)
                     /locus_tag="PK849_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE66912.1"
                     /translation="MRLSPSLKTVIADISLVFVALFWGIGFVAMKDALDTFPTFWLLT
                     LRFGSAALLMGILFRRRLAAITKEDIRAGAIIGVFLGFATQTLGLNYTTPGKQAFLTA
                     TYVVIVPFLSWGLRRIFPGALSFFASFICLVGMGMLTLQEGVLSIGKGDTLTLVCAFF
                     FACQLIAIEYFAAKKDPMILTLLQILMVCLLSLPAALVFETWPGFQGGSGLWSIAYTV
                     VFCTIFAFAVQNTAQKFTPSTHTAILLSLESVFGALSGIYFLGEVFTPRMAAGCALIF
                     VAVMLTEAGPSLVRSVSLRVAAAREKA"
     gene            1563..2048
                     /locus_tag="PK849_12095"
     CDS             1563..2048
                     /locus_tag="PK849_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPE66913.1"
                     /translation="MFIFFASFLMYLLLVWSGSGIPMIEVVIGLLMATVVTFAYKTWH
                     PEKRLSKRGLDPRRWFGFVAYLFGPFALGLAKANIDVAKRVITGKIRPGIVKVTPALK
                     SDVARTLLADSITLTPGTLTVDIDDAGAFYIHWLYVEDENPSEEQLYGNFAHWARRLA
                     E"
     gene            2045..2305
                     /locus_tag="PK849_12100"
     CDS             2045..2305
                     /locus_tag="PK849_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HPE66914.1"
                     /translation="MTVHFVWFVAAVMGILAVAVLGRLISGPTVSDRAVALDTFNTFV
                     VGIMLLLAVGYDSVVMVDVAIVYAALSFVGTMFIARYIEGEV"
     gene            2311..>2491
                     /locus_tag="PK849_12105"
     CDS             2311..>2491
                     /locus_tag="PK849_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPE66915.1"
                     /translation="MAFFILVIAALLVSVFFNSLGAVSLYRFPDVYTRLHGATKCTTF
                     GTIFIAVAVVLYAVGR"
BASE COUNT          521 a          658 c          790 g          522 t
ORIGIN      
        1 ggtgcaggac ttcattccca cgccggggag cctgtcgacc tgcatgtact acaccggcct
       61 gaacccgttc acgggggagg ccgtgccctc tgcgaaggag aggaacgagc ggaagatgca
      121 gcgcgtcctg ctgcagtacg cggagccgaa gaaccgcgag gcggtcatac aggcgcttga
      181 agccgccgga aggaaggacc tgatcggtac cgggccgtcc tgcctggtgc agccccagca
      241 ccgcgagccg cagaaaaaaa gcgcgccccg ccccaagggg gggacgcgct ccggaaaacc
      301 ggacggacgg aggtagttca ggctttctcg cgcgccgccg cgacgcggag cgagactgaa
      361 cgcaccagcg acggtcccgc ctccgtaagc atcaccgcga cgaagatgag cgcgcacccc
      421 gcagccatgc ggggggtgaa gacctctccc aggaaataga tgcccgagag cgctccaaaa
      481 accgattcaa ggctcagaag gatggccgtg tgcgtggacg gcgtgaactt ctgggccgtg
      541 ttctgtacag cgaaggcgaa gatggtgcag aagacgacgg tgtacgcgat gctccagagt
      601 ccgcttccgc cctggaagcc cggccatgtc tcgaagacga gcgcggcggg aaggctcaga
      661 aggcagacca tcaggatctg caggagcgtg aggatcatgg gatccttctt cgcggcgaag
      721 tattcgatcg cgatgagctg gcaggcgaaa aagaaagcgc agacaagagt gagcgtatcc
      781 cccttgccga tggagagcac accttcctgg agcgtcagca tccccatgcc gacgaggcag
      841 atgaaggagg cgaagaagga gagcgctccc ggaaaaatcc tccgcagccc ccacgagaga
      901 aaggggacga tgaccacgta ggttgccgta aggaacgcct gtttgccggg cgtggtgtag
      961 ttcagcccga gcgtctgcgt cgcgaagccg aggaagaccc cgatgattgc ccccgcccgg
     1021 atgtcctcct tagtaatagc cgcgaggcgg cggcggaaaa ggattcccat cagcagcgcc
     1081 gccgagccga agcgcagcgt gagcagccag aacgtgggaa aggtgtcgag cgcgtccttc
     1141 atcgcaacga agccgatgcc ccagaagagg gcgacgaaga cgagcgagat gtccgctata
     1201 actgttttca gagatggaga aaggcgcatc aaattcactc ccgaaacggt aaattattga
     1261 gtcccatact acgaataccg gaagagtata gcatcttccg cgaaaaaaaa gatacattga
     1321 gagccttgcc tgcgcggctt cagtcggcgt gtgcgtgaaa cagggggctt ccgcggttgt
     1381 tgtaccgcac ttttcgagtg tgagattttc ctctgctcca tttcccgctg tttctatttt
     1441 gtgagagtat taactttcat ttacaaggac cgaagaggta gtataatgta ttcgatgcat
     1501 ttctttgttc cgcgggggtg tattcgggtg ttcaaaattc tatgtgacgg gaggtggagt
     1561 atgtgtttat ttttttcgcc tcgtttttga tgtatcttct ccttgtctgg tccgggagcg
     1621 gtattccgat gatcgaggtc gtgataggtc ttcttatggc gacggtggtc acgttcgcgt
     1681 acaagacatg gcatccggag aagaggctca gtaagcgcgg gcttgatccg aggcggtggt
     1741 tcggctttgt ggcgtatctt ttcggaccgt tcgcattggg gctggcaaaa gcgaatatcg
     1801 atgtcgcgaa acgagtcatc acgggaaaga ttcgtcccgg catagtcaag gtgacgccgg
     1861 cgttgaagag cgacgtcgca aggacgttgc tggccgattc catcacgctg acgccgggaa
     1921 cgctcacagt ggatatagat gatgcaggag ccttctatat tcactggttg tacgtcgagg
     1981 acgagaatcc ctcggaagaa cagctgtacg gcaatttcgc ccactgggca aggaggttgg
     2041 ctgaatgacg gtccattttg tgtggtttgt tgcggcggtt atgggcattc tcgccgtcgc
     2101 cgtcctcggg cgtcttatct ccggtccgac ggtctcggac agggcggttg ctctggacac
     2161 gttcaacact ttcgtagtgg gcatcatgct gcttctcgcc gtcgggtacg attcggtcgt
     2221 catggttgat gtggccatag tgtacgccgc gctctccttc gtggggacca tgttcatcgc
     2281 ccgctacatc gaaggggagg tgtaacccgg atggcctttt ttattttggt gattgccgct
     2341 ctgttggtga gcgttttttt caactctctc ggcgcggttt ccctctaccg attccccgac
     2401 gtgtacacta ggcttcacgg cgcgacgaag tgcacgacgt tcggtacgat tttcatagcg
     2461 gtcgcggtgg tgctctatgc ggtcggcagg c
//
LOCUS       DAOPKU010000452         2489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48484_length_2489_cov_4.211175, whole genome shotgun sequence.
ACCESSION   DAOPKU010000452 DAOPKU010000000
VERSION     DAOPKU010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48484_length_2489_cov_4.211175"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PK849_12110"
     CDS             complement(<1..624)
                     /locus_tag="PK849_12110"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPE66916.1"
                     /translation="MTKERIGFIGLGIMGKRMALNLLKAGYSMTIRSGSEETRSEFAA
                     KGVQVAQTPAEVAAESDVIITMLPGSPEVEEVVLGENGIIKTVRAGSACIDTSSIDPV
                     TSVRIASELRKKGVFMLDAPVSGGQEKAESGTLSIMAGGDEEVFERCRAILHSMGKDV
                     VLVGGSGAGQIAKLVNQCIVAVNMAIVGEAMCLGKKAGVDPRKIFEAI"
     gene            768..1337
                     /locus_tag="PK849_12115"
     CDS             768..1337
                     /locus_tag="PK849_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPE66917.1"
                     /translation="MAKSVLAVLAEGFEEIEAITPVDVLRRLDVDVTVASLDGLVVKG
                     ARGISVQADTTLAEVAGRTFDLIVLPGGMPGAANLAASSLLGDMLKKQRAEGRMIAAI
                     CAAPAVVLHPLGLLGGEKTSCYPAFQRRLDEVNRTEERLCTGEKLITAAGPGVAMEFA
                     LALAEILKGKEKTTELAKAMLVEGSRCNA"
     gene            1330..2274
                     /locus_tag="PK849_12120"
     CDS             1330..2274
                     /locus_tag="PK849_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPE66918.1"
                     /translation="MHEHPLVGGIEAGGTKIVCAAGTGPGDLSRAVFPSGSDPAAALF
                     AVTDWFREEETRRGLLHAIGIGSFGPVDLDPASPTYGFITSTPKAGWRNTDILGTVRR
                     AFPALPVAFDTDVNAAALGEQRWGAAAGLDDFVYITIGTGIGAGVMAGGRLVHGLVHP
                     EMGHMRIPRIPDDAFEGVCPFHGDCWEGLCSGPAMKKRTGTPAEEISPDSSAWRFEAR
                     YVALALGNIICALSPRRIILGGSVPNGGLLGIERFFKMVRAEVRDVLDGYIVSPALEE
                     GIDGYIVPPLLGADAGVCGALALALDMLHFSDEVTKAP"
BASE COUNT          425 a          787 c          792 g          485 t
ORIGIN      
        1 tatggcctcg aaaattttcc gcggatccac gcccgccttc ttccccaggc acatggcctc
       61 gcccacgatc gccatattga cggcgacgat gcactgattc accagcttgg ctatctgccc
      121 cgcccctgag ccgcccacca ggacgacgtc cttgcccatc gaatgcagaa tggcgcggca
      181 gcgctcgaag acctcttcgt cgcctcccgc catgatggac agcgtgccgg actctgcctt
      241 ctcttgaccc ccgctgaccg gagcgtcgag cataaagacg cctttcttcc gcagttccga
      301 ggcgatgcgt accgacgtca ccggatcgat ggaacttgtg tcgatgcagg cgctccccgc
      361 tcggacggtc ttgatgatgc cgttttcgcc caggacgacc tcctccacct cgggagatcc
      421 cggaagcatg gtgatgatca cgtcgctttc ggcagccacc tcggcgggcg tctgcgccac
      481 ctggacgccc ttcgcggcaa actcgctccg ggtctcctcg ctgccgctgc ggatggtcat
      541 ggagtacccc gccttgagca ggttcagcgc catgcgcttc cccatgatcc ccaggccgat
      601 gaatccgatt cgttctttcg tcatgccgta ccgtcccttc tgttgaagtg cgcgctgtgc
      661 ttcatcattg tatcatctgc taatggacgg cacgagccgc cgctcgggta taatggaaag
      721 aaaggccggg aacggccgac cggtttcata aagaaggagg agtgaagatg gcgaagagtg
      781 tgctcgctgt gcttgccgag gggttcgagg agatcgaggc gattacgccg gtcgacgtgc
      841 tgcggcggct tgatgtcgat gtgacggtcg cgtcgcttga cgggctcgtg gtgaaggggg
      901 cccggggaat ttctgttcag gccgatacga cgcttgcgga ggtcgccgga cggaccttcg
      961 acctgatcgt gctgccgggc ggaatgcctg gggcggcgaa tctggctgct tcctcgctgc
     1021 tcggcgatat gctgaaaaag cagcgtgcgg aggggcggat gatcgcggca atctgcgcgg
     1081 ctcctgctgt ggtgctgcac ccccttgggc tgctcggcgg cgagaagacc tcctgctatc
     1141 cggccttcca gcgccgcctc gacgaggtca accgcaccga ggagcgcctg tgtacgggag
     1201 agaagctgat cactgcggca gggccgggtg tcgccatgga gttcgcgctg gccctcgcgg
     1261 agattctgaa gggcaaggag aagaccactg aactggcgaa ggctatgctg gtggaaggaa
     1321 gccgctgtaa tgcatgaaca tccgcttgtc gggggaatcg aggccggggg gacgaagatc
     1381 gtctgcgccg cggggaccgg accgggcgat ctgagccggg ccgtcttccc ctccgggagc
     1441 gaccccgccg ccgcactttt cgccgtgacg gactggttcc gcgaggagga gacgcgccgg
     1501 ggattgttgc acgccatcgg catcggctcg ttcggccctg tggaccttga tcccgcgtcg
     1561 cccacctacg ggttcatcac ctcgacgccg aaggcaggat ggcggaatac cgacatactt
     1621 ggaacagtgc gccgtgcttt ccctgctctt cccgtcgcct tcgacaccga cgtgaacgcc
     1681 gcggcgctgg gcgagcagcg gtggggggcg gcggcaggtc tcgacgactt cgtctacatc
     1741 accatcggca ccggcatcgg cgcgggggtg atggcgggcg gacggctcgt ccacgggctt
     1801 gttcaccccg agatgggaca catgcgcatc ccccggattc cggacgacgc cttcgagggg
     1861 gtctgccctt tccacggcga ctgctgggag gggctctgct ccggtccagc catgaagaag
     1921 cggacaggaa cgcccgccga ggagatctcc ccggattcct ccgcatggcg cttcgaggcc
     1981 cgctacgtcg cgctggccct cggcaacatt atctgcgcgc tctccccccg caggatcatc
     2041 ctgggcggga gcgtgccgaa cggagggctt ctcggcatcg aacgcttttt taaaatggtc
     2101 cgcgccgagg tccgtgacgt cctcgacggc tacatcgtct cccccgcgct cgaagagggt
     2161 atcgacggct acatcgttcc ccccctcctc ggggccgacg ccggagtctg cggcgccctc
     2221 gccctcgcgc tcgacatgct ccattttagt gatgaggtca cgaaggcgcc atagcaaaag
     2281 agacaagctc cggtgattcg tcgtataata tgtaagaaga ctgagaaagg aggcgcttcg
     2341 catgaacagc aacgagaaga gagaaaaatc gcgaaggccc ctcgtcgcag tggccggcaa
     2401 ggggtatctt cgcgattttt ctcttttctc gttgctgttc atgcgaagcg cctcctttct
     2461 cagtcttctt acgtattata cgacgaatc
//
LOCUS       DAOPKU010000453         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48555_length_2486_cov_6.090086, whole genome shotgun sequence.
ACCESSION   DAOPKU010000453 DAOPKU010000000
VERSION     DAOPKU010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48555_length_2486_cov_6.090086"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PK849_12125"
     CDS             <1..422
                     /locus_tag="PK849_12125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPE66919.1"
                     /translation="QEMKASAKTGSPLSVLIFDLDHFKMVNDDFGHQAGDNVLRRTAE
                     ILKSLVPVGFVGRWGGEEFLAVLPGAGEEEATEFGERIREAVENEPFPIGRRVTISAG
                     VTEMIPGDTPGSLVGRADKGLYEAKETGRNRVVLRKA"
     gene            complement(433..1122)
                     /locus_tag="PK849_12130"
     CDS             complement(433..1122)
                     /locus_tag="PK849_12130"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE66920.1"
                     /translation="MENENINVNATEKAGREIVRLIASHRFKPGDRLLETSLAKILGM
                     SRTPVREALGRLASTGFLAKAEGTRGYQIPFLTPKDIEDVFFMRGILESKAAMLCAQT
                     MPESALVELRRINEREEETFRANEKGEYAELNESFHMLIAGSCANPYMEMYLRQLYWR
                     SNLYVLFFMSFYCLDSHEETERLSYLEHRRVIDALAGRDAKAAEAAMREHITSSYNHL
                     LYPKNALAGGE"
     gene            1394..>2486
                     /locus_tag="PK849_12135"
     CDS             1394..>2486
                     /locus_tag="PK849_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPE66921.1"
                     /translation="MEERKAREISLRGALMIFGAILAIIVVGRLIFTFDVALLLMFIG
                     MFTTFTYVYVYKFTWRDLFEGGVVPMIARASGAIMILLTVGPLIASWMLAGTIPYLIY
                     TGLQILSPNTFLMAAFVICALSSTMTGTSWGTAATFGVAFMGIAQGLGVPLPAAAGAV
                     VAGAYFGDKLSPVSDTTVLAAAVAEVDVVDHIRSMLWTTIPAFVISLGIYGYVGSQAS
                     GEIDLSSVNEIIAGISKTFKMTPLVLVPPLAVLVLAYRRFPTLPVLWIAVALAVPLAM
                     MQGYDLKEIMRAMAAGPKIVTEVASLDKLLSRGGLAFMSGSVVVVFFAYIFAGQLEYT
                     GTFRRICTSLKERFIGNSKGRFVLSASLSG"
BASE COUNT          444 a          800 c          724 g          518 t
ORIGIN      
        1 aacaggagat gaaggcatcc gcgaagacgg gctcgccgct ctcggtgctc atcttcgacc
       61 tcgaccattt caagatggtg aacgacgatt tcggccacca ggcgggggac aacgtgctcc
      121 gccgtaccgc ggagattttg aagagcctcg tcccggtggg gttcgtcggc cggtgggggg
      181 gcgaggagtt tctcgcggtc cttcccggcg cgggcgaaga ggaggcgacc gagttcggcg
      241 agcgcatccg ggaggctgtg gagaacgagc cgttccccat cggccgcaga gtgaccatca
      301 gcgcgggagt tacggagatg atccccggcg acaccccggg atcccttgtc ggtcgcgccg
      361 acaagggact ctacgaagcc aaggagaccg ggcgcaacag agtggtgctc cggaaggcgt
      421 gaggcccgcc gtctattcgc ccccggccag cgcgttcttc ggatagagca ggtggttgta
      481 gctgcttgtg atatgctctc gcatcgcggc ctccgcggcc ttcgcgtcgc gcccggcgag
      541 cgcgtcgatc acgcgccggt gctcgagata gctcaggcgc tccgtctctt cgtgcgaatc
      601 caggcagtag aagctcatga agaagaggac gtagaggttc gaccgccagt agagctgcct
      661 gaggtacatc tccatgtacg ggttggcgca ggagccggcg atgagcatgt ggaacgactc
      721 gttcagctcc gcgtactccc ccttctcgtt cgccctgaac gtttcttcct cgcgctcgtt
      781 gatccggcgc agctcgacca gcgcgctctc cggcatcgtc tgcgcgcaga gcatcgccgc
      841 cttgctctca aggattcccc gcatgaagaa gacatcctct atatccttcg gcgtcaggaa
      901 ggggatctgg taccccctcg ttccctccgc cttcgccaga aaacctgtgg acgccagcct
      961 tcccagcgcc tccctgacgg gcgttctgct cattcccaga atcttggcga gcgacgtctc
     1021 cagcagacgg tctcccggtt tgaaacgatg cgacgcgatg agccggacga tctcccgccc
     1081 ggccttctcg gtcgcgttga cgttgatgtt ctcgttttcc atgaagcacc tcgcaatttt
     1141 ctcttttccc ttacgaaggg actccaaaag tgtagcagat atccgataaa gatgcattct
     1201 tccggacgcc ttttttaaaa aaactcttga caaaacgctc gcgagagata atgattatac
     1261 aataacgtat ccataaaaga atccagaaag ggggagtgaa acgccgagaa gagtgaaaac
     1321 agcttctccc tacgtcaagt tcgtccgcat ttatttctta tgagccactc ggcacaagga
     1381 ggttatcgta cacatggaag aacggaaagc acgtgaaata tcgctccggg gcgcacttat
     1441 gatttttggc gccatcctgg cgatcatcgt cgtcggaagg ctgatcttca cattcgacgt
     1501 cgccctgctc ctgatgttca tcggcatgtt caccaccttc acgtacgtgt atgtctataa
     1561 gttcacctgg cgcgacctct tcgagggggg cgtcgttccc atgatcgccc gcgcgtcggg
     1621 cgccatcatg atcctgctca ccgtcggccc gctcatcgcg tcgtggatgc tcgcgggaac
     1681 catcccctac ctgatctaca cgggacttca gatcctcagc ccgaacacgt tcctgatggc
     1741 ggccttcgtc atctgcgccc tctcgtccac gatgacggga acctcgtggg ggactgccgc
     1801 aaccttcggc gtcgccttca tggggatcgc gcaggggctc ggcgtacccc ttccggcggc
     1861 cgccggcgcg gtggtcgcgg gcgcctactt cggcgataag ctcagccccg tctccgacac
     1921 cacggttctc gccgcggcgg tcgcggaggt cgacgtggtg gaccatatcc gctccatgct
     1981 ctggaccacc attccggcgt tcgtcatcag cctcggcatt tacggctacg tcggatcgca
     2041 ggcctccggc gagatcgatc tcagcagcgt gaacgagatc atcgccggca tctcgaagac
     2101 cttcaagatg acgccgctcg tcctcgttcc gccgcttgcc gtcctcgtcc tcgcgtaccg
     2161 gcgtttcccg accctcccgg tgctctggat cgccgtcgcg ctcgccgtgc ctctggccat
     2221 gatgcagggg tacgacctga aggagatcat gcgcgcgatg gccgccggcc cgaagatcgt
     2281 caccgaggtc gccagtctcg acaagctcct cagcaggggc ggactcgcct tcatgtcagg
     2341 ttcggtggtc gtggtcttct tcgcgtacat cttcgcggga cagcttgagt acacgggtac
     2401 cttccgccgt atctgcacct ccctgaaaga gcgcttcatc gggaacagca agggacgctt
     2461 cgtcctctcc gcctcgctct ccggca
//
LOCUS       DAOPKU010000454         2483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48644_length_2483_cov_4.173394, whole genome shotgun sequence.
ACCESSION   DAOPKU010000454 DAOPKU010000000
VERSION     DAOPKU010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48644_length_2483_cov_4.173394"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1090
                     /gene="nadE"
                     /locus_tag="PK849_12140"
     CDS             359..1090
                     /gene="nadE"
                     /locus_tag="PK849_12140"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPE66922.1"
                     /translation="MSFKCRDAARLASSIEEWLTQKVHAAGAVGGIVGLSGGIDSAVV
                     AALLKRAFGSEMLSVLLPCGSIPKDKEDALLAVKAFDLPYREVDLSSVYDAFVHAFGE
                     PLSPMAAANIKPRLRMNALYALAQTRRFLVCGTGNKAEILMGYFTKYGDGGCDLLPLG
                     DLLKSEVRALAGFLKVPDEIIQKAPSAGLWDGQTDEEEMGMTYEEIDAYIATGKGDEK
                     VRTRIEQMKNVSAHKRAMPPVCIPF"
     gene            1124..1876
                     /locus_tag="PK849_12145"
     CDS             1124..1876
                     /locus_tag="PK849_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPE66923.1"
                     /translation="MSGHSKWANIKHRKGAQDAKRGNLFQKLVKAIIIAAKEGGGDPS
                     MNMRLKAAIERAKAASVPNDNITRGIKRGTGEIDGGQFDEILYEAYGPNGVAILIEVL
                     TDNRNRAASEMRTLLTRNGGSLGEAGSVAWMFERKGVIEVSGKDLDEEALMTVALEAG
                     AEDLQNQDDGFIVYSEPSIYGLVRDALEKAGYVVSRAETEMVPKNTVMIANKEKAQKI
                     LRLMDLIEEHDDVQNAYGNFDIPDEILESLDA"
     gene            1880..2407
                     /gene="ruvC"
                     /locus_tag="PK849_12150"
     CDS             1880..2407
                     /gene="ruvC"
                     /locus_tag="PK849_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPE66924.1"
                     /translation="MLKCIGIDPGLGRMGVATVLQDGRSFRLISGGCIETSPGEEISS
                     RLDTIFRKLGEQIECCSPDFMSVEKLFFGRNITTAEYVWQARGIALLLAARYRLPLFE
                     PKPSQIKLTVCGYGTAPKEQVQKMVQRLLGLKEVPKPDDVADAIGAAITGFSLFAEKR
                     RISSAGGYHASLSRR"
BASE COUNT          595 a          583 c          719 g          586 t
ORIGIN      
        1 ggtgtggagt tgttatgctg tttcccttct gttgatgttt atgaagcgtt ccctttcgat
       61 caaatcgctc gcgtcagggc agtagagaat cagatattta ccgctcttgt tccgccttct
      121 ggagagagtg ttgtatgctg gggaccaaca ggcgcgcccc tcggttcttc ccggagcgac
      181 tccggagagc ttctcttcgt gttgcggccg gaatacatag cacgcgcacg gaaaaaatat
      241 cctcttcgtt cccttcgaaa aaaacagctt cgctatgcac ttgcagccct ctgatatgaa
      301 cgactatgct aaaataaaca ggttatggat tttcttttag aagaaggtgg atatgtcgat
      361 gtcgttcaaa tgccgagacg ccgctcgcct cgcgagctcc attgaagagt ggctcacgca
      421 gaaggttcac gctgcggggg ccgtcggagg aattgtcggc ctgagcggag gtatagactc
      481 cgccgtcgtg gctgcgcttc tgaaacgagc ctttggctcc gagatgctat cggtgctgct
      541 tccatgcgga agtatcccga aagacaagga ggacgctctc ctcgctgtca aagcattcga
      601 tcttccgtac cgggaggttg acctgtcttc tgtatacgac gctttcgtac atgcctttgg
      661 agaacctctt tctccgatgg ctgcagcgaa cataaaaccg aggcttcgta tgaatgccct
      721 ctatgccttg gcgcagaccc gccgtttcct cgtatgcggc acgggcaaca aggccgagat
      781 tctcatgggg tattttacca agtacggaga tggcggctgc gatcttcttc ctctcggtga
      841 tcttctgaag agtgaagtcc gtgcgctcgc cgggttcctg aaagttccgg atgagattat
      901 ccaaaaggct ccttcggcgg gtctttggga cggacagacg gacgaggaag agatgggcat
      961 gacgtacgaa gagatagacg cctacattgc cactggaaag ggagatgaaa aagtcaggac
     1021 acggattgaa caaatgaaaa acgtgtcggc gcataaaagg gcgatgccgc ccgtgtgtat
     1081 tccattctga gagtgtatat attttccaga ggagtgattt tctttgtcgg gacattctaa
     1141 atgggcgaat atcaaacata gaaaaggagc gcaggatgca aagcgcggaa accttttcca
     1201 gaagcttgta aaagccatca ttatcgccgc gaaagagggc gggggcgatc cctcgatgaa
     1261 tatgcgcctg aaggccgcca ttgaacgggc gaaggctgcg agcgtaccca acgacaacat
     1321 aacgagagga attaagcgcg gtaccggtga aatcgatggc ggacagttcg acgagattct
     1381 ttacgaggca tacggcccga acggcgtcgc cattctgatc gaggttctta ccgataacag
     1441 gaacagggct gcatccgaga tgcgcaccct tctgacaaga aacggcggtt cccttggtga
     1501 agcaggaagc gttgcgtgga tgttcgagcg gaaaggcgtc attgaagttt cagggaaaga
     1561 tcttgacgaa gaggccttga tgacagttgc cctggaggct ggagcggaag acctgcagaa
     1621 tcaggacgac ggctttattg tatacagtga gccgagtatt tacggtctgg tgcgggacgc
     1681 cttggagaaa gcaggctatg ttgtcagccg ggccgagacc gagatggtcc cgaagaatac
     1741 tgttatgata gcaaataagg aaaaagcgca gaagattttg cgtcttatgg atcttatcga
     1801 agaacacgac gacgtccaga atgcctacgg caacttcgac attccggatg aaattctgga
     1861 aagcctggat gcgtaagcgt tgctgaagtg tatcgggatc gacccggggc tgggccggat
     1921 gggagtggca acggtccttc aggatggacg ctctttccgt cttatctcgg gcgggtgtat
     1981 tgaaacatcg cccggagagg aaatttcttc ccgactggac acgatcttca gaaaactcgg
     2041 ggagcagatt gaatgctgct cccctgattt tatgtcggtt gaaaagcttt ttttcggaag
     2101 gaacatcact actgcggagt acgtgtggca ggcacgcggc atagcgctcc tcctcgcagc
     2161 ccgttaccgt ctaccccttt ttgaaccaaa accctctcag atcaagttaa cggtatgcgg
     2221 gtatggaacg gcgccgaagg aacaggtgca gaaaatggta cagcgcctct tggggcttaa
     2281 agaagttccc aagccggacg acgtcgctga tgctatagga gctgcaatta ccggtttttc
     2341 gctttttgcg gaaaagcggc gtatttccag tgcaggaggc tatcatgctt cgctttctcg
     2401 caggtaatgt tcactcgata attgacaata tggtcgtgct ggaagtcaac ggtttgggtt
     2461 tggaactcct ttgtacaggg cgt
//
LOCUS       DAOPKU010000455         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48803_length_2478_cov_3.046636, whole genome shotgun sequence.
ACCESSION   DAOPKU010000455 DAOPKU010000000
VERSION     DAOPKU010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48803_length_2478_cov_3.046636"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="PK849_12155"
     CDS             complement(<1..1821)
                     /locus_tag="PK849_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE66925.1"
                     /translation="MEVSRRVSTKAILLGNEAIARGIVEAGCLVASAYPGTPSSEILP
                     AVAKFADEMKSPMSVEWSVNEKVAFEVAAAASFTGARACAVMKQVGLNVAADPFMSVA
                     HFDLKGGFLLVVADDPGPHSSQTEQDSRYFAMFAKIPCFDPATAAEAKEMVFDAFDLS
                     ERFGIVAMLRPGVRVDHCRQDIELGDIRPATGTVKFEKEGTRWVCLPAGVKINHPKLN
                     RKVEDIRKAFETEFSKYNYEVPAKKQPVLGIVASGVPFSVVTDLLKEAGREDVSVLKI
                     GTPHPLPVKMVQDFIARHDRVLFLEETYPVMEMQLPDRTGILGRWNGVVPSAGELLPE
                     VIADILGGLLQDDSLAGKQHPLLKEATEDLNIVPRKPMLCPGCPHRASFFSIRQALPN
                     AINPSDIGCYTLGVNQKAVDAVVDMGASVTMASGFYLAHKAAGIDRPVVTTIGDSTFF
                     HMGLPGLASAVYNRHAFVLAILDNSITAMTGGQANPAVGTKLRKGDEGRKVDIEGACR
                     GCGVEYVKTIAAYDVALGKAAVKEAWEYAKSKGEPAVLIFRHPCMLLRPEQPSIPVKV
                     DQEKCIGCKFCINFFNCPGLVFSEETGKAYIDERFCVSCGV"
     gene            complement(1805..2230)
                     /locus_tag="PK849_12160"
     CDS             complement(1805..2230)
                     /locus_tag="PK849_12160"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPE66926.1"
                     /translation="MSSEIPFTLRVRYGDTDQMGVAYYANYLYWFEVGRTEFCRALGR
                     SYADWEAEGVFLPVVESHCRYKHPARYEDEITIYTKIGELRASSIVFECRIERASDGK
                     PLAAGWTRHAFVDADGRLIRGDNPMRRWIEALRDDGGVA"
BASE COUNT          495 a          779 c          709 g          495 t
ORIGIN      
        1 gacgccgcat gacacgcaga accgttcgtc gatgtacgcc ttccccgtct cctcggagaa
       61 gacaaggccc gggcagttga agaagttgat gcagaacttg cagccgatgc acttctcctg
      121 gtcgaccttc accgggatcg acggctgctc cggacggagc agcatgcacg ggtgacggaa
      181 gatcagcacg gccggttccc cttttgattt cgcgtactcc cacgcctctt tgacagcagc
      241 cttgccaagc gctacgtcgt aggcggcgat ggtcttcaca tactccacgc cgcagccccg
      301 gcaggcgcct tcgatatcga ccttccgtcc ttcgtcgccc ttgcggagct tcgttccgac
      361 ggcggggttc gcctgtccgc ccgtcatggc ggtgatgctg ttgtcaagga tggccagcac
      421 gaaagcgtgg cggttgtaga cggcgctcgc cagtccgggc agccccatgt ggaagaaggt
      481 ggagtctccg atggtggtga cgacggggcg gtcgattccg gcggccttgt gggcaaggta
      541 gaagcctgag gccattgtga cggaagcgcc catatcgacg accgcgtcca cggccttctg
      601 gttgactccg agcgtatagc agccgatgtc ggaagggttg atcgcgttcg gaagcgcctg
      661 gcggatggag aagaagctcg ccctgtgcgg gcagccggga cagagcatcg gcttgcgcgg
      721 cacgatattc agatcctccg tcgcctcttt cagaagagga tgctgctttc ccgcaaggga
      781 atcgtcctgg aggagccctc cgagaatatc ggcgatcact tccggaagca gctcgcccgc
      841 gctgggaacc acgccgttcc agcggccgag aatacctgtg cggtcgggaa gctgcatctc
      901 catgacgggg taggtctctt caaggaagag caccctgtcg tgacgggcga tgaagtcctg
      961 caccatcttc accggcagag ggtgcggcgt gccgattttc agtaccgaga catcctctct
     1021 cccggcttct ttaagcaaat ccgtaacaac ggagaacgga acgccggagg cgacgatgcc
     1081 gagtaccggt tgcttcttcg ccggaacctc gtagttgtac tttgaaaact cggtttcaaa
     1141 agccttccgg atatcttcaa ccttgcggtt gagcttcgga tggttgatct tcactcccgc
     1201 gggaagacag acccagcggg ttccctcttt ctcgaacttt accgtaccgg ttgcagggcg
     1261 aatatctcca agttcgatat cctgacggca gtggtccacg cgcacgcccg gccggagcat
     1321 cgcgacgatg ccgaaacgct ccgagaggtc gaaggcgtcg aagaccatct ccttggcctc
     1381 ggcggcggtc gcagggtcga agcacgggat cttggcgaac attgcgaagt agcggctgtc
     1441 ctgctccgtc tgcgagctgt gcggacccgg atcgtccgcg acgaccagca ggaaaccgcc
     1501 cttcaaatcg aagtgcgcca cgctcatgaa cggatcggcc gccacgttga gcccgacctg
     1561 cttcatcacc gcgcatgccc tggctccagt aaacgaagcc gccgcggcca cctcgaacgc
     1621 caccttctcg ttgacgctcc actccaccga catcggcgac ttcatctcgt cggcgaactt
     1681 cgccaccgca ggcagaatct ccgacgacgg cgtccccgga taggccgacg caaccaggca
     1741 tccggcctcc acaattcctc tcgctatcgc ttcgtttcca agcagtatgg cttttgtgct
     1801 cactctacgc gacacctcca tcatccctca gcgcctctat ccagcggcgc atggggttgt
     1861 ctccccgtat cagcctgccg tccgcgtcga caaacgcatg gcgggtccat ccggcggcga
     1921 gcggttttcc gtcggacgcc ctttcgatgc gacattcgaa gacaatgctg ctcgctcgga
     1981 gttcaccgat tttcgtgtat atggtgatct catcctcgta ccgagcagga tgcttgtacc
     2041 ggcagtgcga ctcgacgacg gggaggaata ctccctcggc ctcccagtcg gcgtacgacc
     2101 tgccaagagc ccggcagaac tccgtccgcc cgacctcgaa ccagtagaga tagttggcgt
     2161 agtacgcgac gcccatctgg tcggtatcgc cgtaacgaac gcgcagtgtg aaaggaattt
     2221 cggaggacac gggaacacct cccggaaaag gtggagtgaa attaacatct tgaaaaacgc
     2281 atgtgatgcg cgcgttttca acaagaactc actccttgtg gtgaattata taccaaatac
     2341 tattttttac gcaacaaaaa aatcatacac cgcacttatt catatgcgcc gccataagac
     2401 agtcttcttc caccacagga taccccgccc cggcgagcga ggatgcaagc ggctcattct
     2461 gcgctcccgg ctggaacc
//
LOCUS       DAOPKU010000456         2473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48944_length_2473_cov_4.964847, whole genome shotgun sequence.
ACCESSION   DAOPKU010000456 DAOPKU010000000
VERSION     DAOPKU010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48944_length_2473_cov_4.964847"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PK849_12165"
     CDS             complement(<1..561)
                     /locus_tag="PK849_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66927.1"
                     /translation="MAKKKRPSLKDYLSTGQVIAEEVQEQSVSLEEAPAAPPAGEPSA
                     PEKKPAKARKPRSSAAKIGAAKKKSPVPDESAGPSTGESSSPDEGSVVLQEPSGELRS
                     FVDMLCGEDRKTWEKIFAVTGAEFLPLDFVERREDFRTMDRSRFTLFKLEEAGKPLFH
                     VRTSVQIREPLIALLKWDETGKATIYR"
     gene            complement(554..1321)
                     /locus_tag="PK849_12170"
     CDS             complement(554..1321)
                     /locus_tag="PK849_12170"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPE66928.1"
                     /translation="MKIVGFCNLKGGVGKTTACQNLAVALVRRGLKVAALDLDPQSNL
                     TAGFGISIPEDRPYIYDFLMGDVSFGDLKTVREGVDVAPSTLDLAVVEMQLESQPGRD
                     TLLKDALASSDLSEYDVVFCDSPPQLGIFTRNVLAACGEILVPLESEFYSLAGLRLLS
                     QTVELYKKRLNRGLQISGILLTRHNPKIIMNREVEKEVRSHFGDTVFRRYIRQNISLV
                     EASGAGLSVFGYDEESNGARDYGVLADELLERWKKNG"
     gene            1496..>2473
                     /locus_tag="PK849_12175"
     CDS             1496..>2473
                     /locus_tag="PK849_12175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPE66929.1"
                     /translation="MTTASGSRSPRRRHFDGIANVKLLREITRHLFAETTDLLFLFTL
                     EGKLREISRSASDELGYPEEDALSLNFFADLLAVPSPVKMEQLCKSLLDGQDVTLAGT
                     VRRRDGSGCAVQLTVFRPGEGREALFLCSAKKPEEEKPPRDTGQKVALRDPVTGLSTR
                     AFFFEELQQMDSERYLPLSVIRGDLNGMKLINSVFGHIAGDKLLKAGARVLRKHSRNS
                     DILVRWEGDEFLLLLPRTKEDDAATIVERIEDAFKKTKIREIPVPPSISMGYSAKVHR
                     WQDFGNVLKDAEEDMREKQLSESRKIRTKLLSSLQKSLDEHTPETEEHIS"
BASE COUNT          527 a          734 c          696 g          516 t
ORIGIN      
        1 cctataaatg gtcgcctttc ccgtttcgtc ccacttcagc aacgcgatca gcggttcgcg
       61 gatctgcacg ctggtgcgaa cgtggaagag cggcttccct gcctcttcca gcttgaagag
      121 cgtaaaacgg ctcctgtcca tcgtacgaaa atcctccctg cgttcgacga aatcaagcgg
      181 caggaactcc gctccagtaa ccgcaaagat cttctcccac gtttttcggt cctcgccgca
      241 gagcatgtcg acgaacgagc gaagctcgcc gcttggctct tgcagtacaa cgcttccctc
      301 atcgggagac gaactttctc cggtggacgg gccggcgctt tcatccggaa cgggagattt
      361 tttcttcgcc gcgccgatct tcgccgcgct gctccgcggt tttcgcgcct tggcgggttt
      421 cttttccggc gcggacggct cgccggccgg aggagccgca ggcgcctctt cgagcgaaac
      481 agactgttcc tgtacctcct cggcaatcac ctgcccggtc gacaggtagt ccttcaaact
      541 cggtctcttt ttcttagcca tttttcttcc acctctccag aagctcgtcg gccagtacgc
      601 cgtagtctct ggcgccgttg ctctcctcgt cgtatccgaa gacggagagc cccgctccgc
      661 tggcctccac gagactgatg ttctgccgga tgtaacgccg gaagaccgtg tcgccgaaat
      721 gactgcggac ttctttttcc acttcgcggt tcatgatgat tttcgggttg tgcctcgtca
      781 gaagtattcc gctgatttga agcccccggt tcaggcgctt tttgtagagt tccaccgtct
      841 gcgagagaag acggagtcct gcgaggctgt aaaactcgct ctcaagcggg acaagtatct
      901 ccccgcaggc ggcaaggacg ttccgtgtga aaatgccgag ttgcgggggg ctgtcgcaga
      961 agacgacgtc gtactccgaa aggtcggacg aggcgagggc gtccttcagc agcgtatcgc
     1021 gtcccggctg gctttcgagc tgcatctcca ccacagcgag gtccagcgtg ctcggcgcca
     1081 catccactcc ttcgcggaca gttttcaggt ccccgaaaga aacgtctccc ataaggaagt
     1141 cgtagatata cggacggtcc tccgggatag agatgccgaa gccggcggtg agattgctct
     1201 gcggatcgag gtcgagcgcg gctactttca gtcctctgcg gacaagagcc accgcaaggt
     1261 tctggcacgc ggtggtcttg ccgacaccgc ctttgagatt gcaaaaaccg acgattttca
     1321 tgaagaatcc ccccggacaa atgtgcgatc aaagcatcca gtcgagtata taacataacc
     1381 tcgcaatata gcatagcaag aaacggctgt tgcgaaaaga cgtctttttt cgcagtgtag
     1441 agtataattg gtcaactaca tgcgattctt cgccatctaa aaggaggcgg cacttatgac
     1501 gacagcgagc ggctcccgtt ccccgcgccg gcggcatttc gacgggatcg ccaacgtgaa
     1561 actcctgcgt gagattacgc ggcatctttt cgcggagaca accgacctgt tattcctctt
     1621 caccctcgaa gggaagctgc gcgagatcag ccgatcggca agcgacgagt tgggataccc
     1681 cgaagaggac gcgctctcgc tgaatttttt cgccgatctt ctcgccgttc cctctccggt
     1741 aaaaatggaa caactctgta aatccctcct cgacgggcag gatgttaccc tcgcaggaac
     1801 agtccggcgg cgcgacggaa gcggctgcgc agtgcagctc acggtcttcc gcccgggcga
     1861 gggaagagaa gcgctctttc tttgttcggc gaaaaagccc gaagaagaaa agcccccccg
     1921 cgacaccgga cagaaagtcg cgctacgcga tccggtaacg gggctctcca ctcgcgcctt
     1981 tttcttcgag gagctgcaac agatggactc tgaacgctac ctgccgctga gcgtcatccg
     2041 cggagatctg aacggcatga agctgatcaa cagcgtattc gggcatattg cgggggataa
     2101 gctcctcaag gccggggcca gggtcctccg gaaacattcg agaaacagcg atatcctcgt
     2161 ccgctgggag ggcgacgaat ttttgcttct gctcccgagg acgaaggaag atgacgctgc
     2221 aacaatcgtc gagcgcatag aggacgcgtt caaaaagaca aaaatcagag agatcccggt
     2281 tcctccgagt atttccatgg ggtactccgc caaagtgcac cgatggcagg acttcggcaa
     2341 cgtcctcaaa gacgcggagg aggacatgcg cgagaagcag ctgtcggaga gcaggaagat
     2401 ccggacgaag ctgctttcgt cgctccagaa gtcgctcgac gagcatacgc cggaaaccga
     2461 ggagcatatc agt
//
LOCUS       DAOPKU010000457         2471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_48987_length_2471_cov_4.732202, whole genome shotgun sequence.
ACCESSION   DAOPKU010000457 DAOPKU010000000
VERSION     DAOPKU010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48987_length_2471_cov_4.732202"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..597
                     /locus_tag="PK849_12180"
     CDS             58..597
                     /locus_tag="PK849_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66930.1"
                     /translation="MPGGKSFRPDDILRIRNGKTVEIENTDAEGRLLLADALCFACEQ
                     KPDAIIDMATLTGACAVALGQNMAGLFTPDDAFADGFLEVSARRGERFWRLPMDDDRI
                     AEAMKSPVADLVNCGSRYGGAIFAAQFLKEFISDGIPWIHLDIAGVDMQKDEHSVYSK
                     GATGFGVRSCLEYLLSISK"
     gene            complement(632..2040)
                     /gene="mnmE"
                     /locus_tag="PK849_12185"
                     /pseudo
     CDS             complement(632..2040)
                     /gene="mnmE"
                     /locus_tag="PK849_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
     gene            complement(2228..>2471)
                     /locus_tag="PK849_12190"
     CDS             complement(2228..>2471)
                     /locus_tag="PK849_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPE66931.1"
                     /translation="VKPEGGFFYWLNVPSVNTNDLFKKAIEKKVAFVPGAPFCPVEGS
                     GLHNARLCYTFPSPEIIEDGVARLAEAIGELAIPAL"
BASE COUNT          542 a          644 c          708 g          577 t
ORIGIN      
        1 tgtgcttggc attgatgcaa aaatactttt aggctttaag gtaggtaagc tgaaaacatg
       61 cccggcggca aatcgtttcg cccggacgat atactgcgta tccggaacgg caaaactgtg
      121 gagatagaga atacggacgc ggagggacgg cttcttctcg ccgacgcgct ctgttttgcc
      181 tgcgaacaga agcccgatgc gatcatcgac atggcgacgc tcaccggagc gtgcgcggta
      241 gccttgggac agaacatggc gggccttttc actcctgacg acgcgtttgc cgatgggttt
      301 ttggaggttt ctgcgaggag gggagagcga ttctggcgtc ttcccatgga tgacgaccgg
      361 attgcggaag cgatgaagtc tccggtagcc gatctcgtca actgcggcag cagatacggc
      421 ggagcaattt ttgcggcgca gttcctgaaa gaattcatca gcgacggaat accttggata
      481 catttggata ttgcaggcgt ggatatgcaa aaggacgagc attcagtcta ttccaagggg
      541 gcgacgggtt tcggcgtccg gagctgtctg gagtatcttc tttcgatttc aaaataagaa
      601 atggaaaaga gggggactta agccccctct tttatttccc cacgcagaac tggctgaaaa
      661 tatagtcgag gagggagtcg tccggggcaa ttcccaacaa gcgttccatt gacagacggc
      721 tttcggtaag acaggaagtc gccgcgtcct gccctaaccc tccggagagc gcgttcgagg
      781 catcttttag agagcctatt gctgcgcgaa tttcctctac ctgcctggct gagacgttca
      841 atccggcgtc aagcgtaccc gcgccggcga tggaggagac gatagcgtcc ttcagcgaat
      901 cgattcccgt cccatgtttt gcagagattg aaaggagcgt acttccgggc agcatttcag
      961 cgagcagctc ctccgatgtg tgttcttcgg catttttcgc ccgcggtagg tcggctttgt
     1021 ttaccgcaac gacatgttca agtcctgcaa tacgctccga catggactgg tcgaattcat
     1081 cggggaggga acttccgtcc acaacccaca ccctgacgtc ggctttttcg aaggtagctt
     1141 cagcgcgggc aatccccagg gcttctactt cgtcggaggg cgtcccgccc atacccgccg
     1201 tatcgacgag tcggagcgga atcccgcgat aggtgagtac ttcttcaatc agatcgcgtg
     1261 ttgtgcccgg aattgatgtg acgattgcgc gggattcttt aaggagcgcg ttgagcaggg
     1321 atgatttccc tacgttcggc cgcccgacaa tagccaccct gataccctcc cggagaagaa
     1381 atccggtatt gcaccggtca agaaggtctt caagactttg acaaagagca taaatggatt
     1441 ggagaatctc atcgtcatca tggagaggaa tgtcctcctc gggaaaatcg agcgctacct
     1501 ccatccgggc ggagagagcg agaagttctt gatggatatc catagcaaat gaggcgagtt
     1561 caccgcggag cgttctggtg gcggctcgaa gcgcttcctc actcttcgag cggatgatgc
     1621 cgagtaccgc ttcagcctga gtcaagtcga tccgtccgga gagaaacgcg cgtttggtga
     1681 attctccggg ttcagcaagc cgtgcgcctc tgcaaacgag gagttcaaga cacttcgagg
     1741 cgacgagcgt tcccccgtgc gtatgtattt ccacgacgtc ctctccggtg taactcttgg
     1801 gagcggcgaa ccagacggca agcacctggt caagggcttc tcccttctcg tcgagaaggt
     1861 acccgttgcg catccggcga gcaggtgttt cagctaaagg gacggtaagc gagagcgttc
     1921 tgtcggcgat ctctttggac cgcgccccgg aaagccgtac gacggctatt cccgcttccc
     1981 ccgggccgtt gcgattgcgg cgatggtttc ctcttgaaac gacggattga ccgggcgcat
     2041 gttcttcctc ctctcaaagt ggatttttgc tgtctgtgtc ccgtattgta ccgcaaaagc
     2101 gctgtactaa aaacgaaaac ctctccctaa atcttcattt cgggagaggt ttttctgttc
     2161 aagatagaga gagtatagcg aagagagagt aaatcagtct ttcgtgcggc tgttgtttct
     2221 ttttgcttta cagcgccgga atggcgagtt ctccaatggc ctccgccaaa cgtgccacgc
     2281 cgtcctcaat gatctcagga ctcgggaagg tgtagcaaag gcgtgcattg tgcagtccgg
     2341 acccctctac aggacagaag ggagcgccgg gaacgaaggc gacttttttc tcaatggctt
     2401 ttttgaaaag gtcattcgta tttactgaag gcacgttcag ccaatagaaa aagcctccct
     2461 cgggtttgac c
//
LOCUS       DAOPKU010000458         2463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_49199_length_2463_cov_4.479651, whole genome shotgun sequence.
ACCESSION   DAOPKU010000458 DAOPKU010000000
VERSION     DAOPKU010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49199_length_2463_cov_4.479651"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..837
                     /gene="nikR"
                     /locus_tag="PK849_12195"
     CDS             430..837
                     /gene="nikR"
                     /locus_tag="PK849_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPE66932.1"
                     /translation="MNSLIRFGVAVPSTLLEEFDSFIERKGMPNRSEALRQLIRERLS
                     REMWVSGSGVVYGTVTMMYDHHGKDVVAALTALQHEYGESIICTTHVHVDHHHCLECI
                     VLKGDAGAIRRFVEALATLKGVKSIEPSISAIL"
     gene            complement(880..1062)
                     /locus_tag="PK849_12200"
     CDS             complement(880..1062)
                     /locus_tag="PK849_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66933.1"
                     /translation="MTEPEKYRIFRCASCGTEFRVEGAGSARCTSCGSKVLILLEGES
                     LSRKKCSGCTASSCST"
     gene            complement(1099..2049)
                     /gene="argF"
                     /locus_tag="PK849_12205"
     CDS             complement(1099..2049)
                     /gene="argF"
                     /locus_tag="PK849_12205"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPE66934.1"
                     /translation="MALNLKGRSFLTLMDFTPEEIYYLLDLSADLKAKKRSGIRGTAL
                     QGKNVALIFEKSSTRTRCAFTVACIDEGAHPEFLSKNDIHLGAKEDVKDTARVLGRMF
                     DGIQYRGFKQSLVEELAQYAGVPVWNGLTDVDHPTQILADFLTIQENFGKQLKGIRLV
                     YCGDGRNNMSNALMIGCAKLGMHYVVASPKALFPEKELLAKCSAMASETGGSVTVFED
                     PLVAVKGADAIYTDVWVSMGEEEKKKERQELLTPYQVNMKLLKASGKDSTIFLHCLPA
                     VKGQEVTEDVFEAPNSKVFDEAENRMHTIKAVMVATIGNQ"
     gene            complement(2056..>2463)
                     /locus_tag="PK849_12210"
     CDS             complement(2056..>2463)
                     /locus_tag="PK849_12210"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPE66935.1"
                     /translation="LRISLTEKPEEEVLAGYALLRALDIRSHGVTIVSCPTCGRRRID
                     VRSILEKLEPSFADLPDALTVAVMGCEVNGPREAMDADFGVAGSPGGAVIFKKGKVLR
                     EVSLDELPGVFADFACSSGENHPSNSCEHLKGE"
BASE COUNT          580 a          680 c          624 g          579 t
ORIGIN      
        1 acaactccac acctgacaaa gcaagcgctc gactatgttc agggatgagc agatccatac
       61 ctcctgaaag accacagaga gagccttgaa cagaaaaagt ggaaaagccg actcgccgac
      121 cgggcagctc tgccggggag ctgattccgc tgtaccgcaa caattcgttt ccaccgtcgt
      181 cgcatacgag cagttcgcct gaaagcattt cattccttgc cgcacaaaaa gctatcctgg
      241 acaacgaggt gcaagaaagc gtttttgcag aagaaaaagc gaaaaggacg agacatgctg
      301 cgctctgaat ctccccgtcg ggtgagtgca gcgcgaacgc ggcggaattt ggatacaaat
      361 gaagcgcccc ccttaaaggg aatttggttt attatactcc atgggggaaa agacaaagga
      421 ggagtatttg tgaactcatt gatccgtttc ggcgtcgcgg taccatccac actcctggaa
      481 gaattcgatt catttatcga aagaaaaggg atgcccaacc gctccgaggc actgcgtcaa
      541 ctcattcgtg aacgcctttc gcgagagatg tgggtcagcg gttccggggt tgtctacggg
      601 acagtgacca tgatgtacga ccatcacgga aaggatgtcg ttgcggcgct cactgcgctg
      661 caacacgagt acggcgagag catcatctgt acaacccatg ttcacgtcga ccatcatcac
      721 tgcctggagt gtattgtttt aaagggagac gccggagcga tacgccgttt tgtggaggcg
      781 ctcgccacgc tcaaaggcgt taagagtatt gagccgtcga tttcggcaat cctttaatag
      841 tgaaaggggc agcggaccgc tgccccgatt ttatacgcct caggttgagc aagaggaagc
      901 cgtgcatccg ctgcactttt tccgggaaag actctcgcct tcgagcagga tgagcacctt
      961 gctcccgcac gaggtgcatc gtgcgcttcc ggcgccctcg acacgaaact ccgtcccgca
     1021 tgaggcgcat cggaagatcc tgtatttttc gggttctgtc attggcttca cctcccagtc
     1081 gtactcagag acacgtagct actgattccc gattgttgcc accataacag ctttgatagt
     1141 gtgcattctg ttctccgctt cgtcgaagac ttttgaattc ggcgcctcga agacgtcctc
     1201 ggtcacttcc tgccccttca ctgcaggaag gcaatgcaag aagattgttg aatcctttcc
     1261 ggacgccttc aacagtttca tattcacctg atacggcgta agcaactcct gccgttcttt
     1321 cttcttctct tcttcgccca tcgaaaccca gacgtcggta tagattgcat ctgcgccttt
     1381 tacagctacg agcggatctt caaagaccgt tacggagccg cctgtctcgg aagccattgc
     1441 cgagcacttt gcaagcagct ctttttccgg gaagagcgcc ttcggagagg ctacgacata
     1501 gtgcattccc aactttgcac acccgatcat cagggcgttc gacatattat tccgtccgtc
     1561 cccgcagtac acaagacgta tacccttaag ctgtttcccg aaattttcct gtattgtcaa
     1621 aaagtctgcg agaatctgcg tcgggtggtc cacatcggtc aatccgttcc atacgggaac
     1681 gccggcatac tgggcgagct cctctaccag cgactgcttg aatccgcggt actgaatacc
     1741 gtcgaacatt cttccaagca cccgggcggt gtccttgaca tcctctttcg cgcccagatg
     1801 gatatcgttc ttgctcagaa attcaggatg ggctccttcg tcgatacatg cgacagtaaa
     1861 agcacagcgg gttctggtgg acgacttttc gaaaataagt gcaacgtttt tcccctgaag
     1921 cgccgttccc cggattccgg aacgtttttt tgccttcaga tctgcggaga ggtccaaaag
     1981 atagtaaatt tcttcgggag taaaatccat cagagtgaga aaactgcgtc ctttgagatt
     2041 gagagccata gtacatcatt ctcctttcag atgttcgcat gaattactcg ggtggttttc
     2101 acccgagcta caagcaaagt cggcgaaaac accgggaagc tcatccaagg agacttcccg
     2161 caggactttt ccctttttga agatgacggc gccgcctggc gagccggcaa cgccgaagtc
     2221 ggcgtccatc gcctctctcg ggccgttcac ctcgcagccc attaccgcga cggtcagcgc
     2281 atcggggaga tcggcaaaag agggttctaa tttttccagg atggaacgca cgtcaatacg
     2341 tcttcgtccg catgtagggc acgaaacgat agtgactccg tgagatcgaa tgtcaagcgc
     2401 tctcaaaagg gcgtatccgg ccagaacctc ttcctccggt ttctcggtaa gactgatccg
     2461 gag
//
LOCUS       DAOPKU010000459         2458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_49352_length_2458_cov_3.421140, whole genome shotgun sequence.
ACCESSION   DAOPKU010000459 DAOPKU010000000
VERSION     DAOPKU010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49352_length_2458_cov_3.421140"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..2311
                     /locus_tag="PK849_12215"
     CDS             146..2311
                     /locus_tag="PK849_12215"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPE66936.1"
                     /translation="MITSYDTQLADILLKAGKRAGLAGEKEIDAAMLLREAGECAGLP
                     VPAPLPDGESLSRPRISEAVKECLDAAVSDSPSGVAGVREFLLALCRSSRIEDGLFAA
                     AGVRNRGALLDRMLAEQQENEQKEARHRAADSVIGRNGRDLTALAKAGKLHMPLMRDT
                     ECAQTINILCRMEKGNPILLGEAGTGKTAIAEGVACLVASGNVPQTLRGARVIEISAS
                     AFTDAFRKGMGEEFVRRLAKEIFADDESGERSILFVDELHILLEVGAMSEFLKPFLAR
                     GGFRMIGATTFKEYRRFIERDEAFSRRFAPVHVEEFTPEQAIKVITGRLSRLEAYHGV
                     RITPEAAEASVRLSHRYIPHRRLPDKALDILDTACVLALSGGEVTQGHIKAAVASAAN
                     LPVEAVSNSFLETCKGLSKRLSGRVKGQRAAVGAVSRLLKRCASGLGGEGNRPLGSFL
                     FAGSTGTGKTELAKAVAESFFGREDKMIRLDMSEYCEPSAVNRLIGSSPGYVGSEQGG
                     VLTEAIRKNPFSLILVDEIEKAAPQVVKLFLQILDEGRLSDASGNSFDFRNALIVFTT
                     NAGAHEKRSSIGFLSEGEQKEQARETLRENMGHVFPREFLGRFDEVIHFDDLDERTLR
                     RIVALQLIAKKKALKGRGITLHVKGSALYALARQAAGSPSGGREVRRLLNAKVYDPLA
                     DLMAEGTANELLVTVSRSSPEMIVVEAFPAQREKERGAA"
BASE COUNT          485 a          796 c          773 g          404 t
ORIGIN      
        1 gggaaagaat tctcattctc ggacatcgag tattgcagcg gtaagtatcc gccattataa
       61 gtttttttca cccccttatt ccgtccctgt tgcctcgtat catagacacg gaagatatcg
      121 aacacaacca caaggaggag ccgccatgat tacatcgtat gacacgcaac ttgcggatat
      181 tctgttgaag gccggaaaac gcgcagggct tgccggggag aaggagatcg acgccgcaat
      241 gctcctccgc gaagcggggg agtgtgcggg gcttccggtt cccgctcctc tccccgatgg
      301 agagagcctc tcccgcccgc gtatatccga agccgtcaag gagtgccttg acgccgccgt
      361 aagcgattcc ccctccggcg tcgccggagt tcgcgaattc cttctcgcgc tctgccgcag
      421 cagccggatc gaagacggcc tcttcgccgc cgccggagtg cgcaaccggg gcgcccttct
      481 cgaccggatg ctcgccgagc aacaggaaaa tgagcagaag gaagcgcgcc accgcgccgc
      541 ggactccgtc atcggacgca acggccgcga cctcaccgcc ctcgcgaagg cgggaaagct
      601 gcacatgccc ctgatgcgcg acaccgagtg cgcgcagacg atcaacatcc tctgcaggat
      661 ggaaaagggg aaccccattc tgctcggcga ggctggaacg ggcaagaccg ccatcgccga
      721 aggagtcgcc tgccttgtcg cctccgggaa tgtgccgcag acgctgcgcg gcgcgcgagt
      781 gatcgaaata tccgcctccg cgttcaccga cgccttccgc aaagggatgg gcgaggagtt
      841 cgtccggcgg ctcgccaagg agatttttgc cgacgacgag agcggggagc gcagcattct
      901 cttcgtggac gagctgcaca tcctgctcga agtgggcgcc atgagcgaat tcctcaagcc
      961 cttcctcgcg cgcggaggtt tccgtatgat cggggccacc acgttcaagg agtaccgccg
     1021 tttcatcgag cgggacgagg cgttctcccg ccgcttcgct cccgtccacg tcgaggagtt
     1081 caccccggag caggcgatca aggtgataac ggggcggctt tcccgcttgg aggcatacca
     1141 cggagtgcgc atcacccccg aagctgcgga ggcctccgtc cgcctttcgc accgctacat
     1201 cccgcaccgc cgccttccgg acaaggcgct cgacatactg gacacggcct gcgtcctggc
     1261 tctgtccggc ggggaagtca cgcaggggca catcaaggcc gccgtcgcct cggcggcgaa
     1321 tctgccggtc gaagccgtca gcaacagctt cctcgagacg tgcaagggac tgtcgaaacg
     1381 cctctccgga agggtgaagg ggcagcgggc tgccgtcggc gcggtctcgc gcctgctcaa
     1441 acgctgcgcg tcaggcctcg gcggggaagg gaaccgcccc ctcggcagct tcctcttcgc
     1501 cggatccaca ggaaccggca agaccgaact cgccaaggca gtggctgagt ccttcttcgg
     1561 ccgagaggac aaaatgatcc gcctcgacat gagcgaatac tgcgaaccgt ccgccgtgaa
     1621 ccgcctgatc ggttcttccc ccggctacgt cggctccgag cagggcggcg tgctcaccga
     1681 ggcgatccgc aagaacccct tcagcctcat tcttgtggac gagatcgaga aggcggcccc
     1741 gcaggtggtc aagctcttcc tccagatact cgacgagggc aggctgagcg acgcctcggg
     1801 gaactccttc gacttccgca acgcgctcat cgtcttcacc acaaacgccg gcgcgcacga
     1861 gaaacgaagc tcaatcggtt tcctctccga aggcgagcag aaggagcagg cgcgggagac
     1921 gctgcgcgag aacatggggc atgtcttccc gagggagttt ctggggcgct tcgacgaggt
     1981 gatccacttc gacgacctcg acgagcggac gctgcgccgc atcgtcgccc tccagctcat
     2041 cgcgaagaag aaggcgctca aagggcgggg aataacgctg cacgtcaagg gaagcgcgct
     2101 ttacgcgctc gcccggcagg cggcgggaag cccctccgga ggccgcgagg tacgccgcct
     2161 gctcaacgcg aaggtgtacg accctctggc cgacctgatg gcggagggga cggcgaacga
     2221 actccttgtc acggtctccc ggagttcccc tgagatgatc gtcgtggagg cgtttcccgc
     2281 acagcgggag aaggagcggg gagccgcata aaagaagaga cgggcggatg gcgccgtaag
     2341 ccgtacgatg ccgtccgccc tttccagggg tatcttactg tttcagggta tcatcacccg
     2401 actgcgagga aaaagcagcg tccgtgcagt cgggtaatga taccctgaaa cagtaaga
//
LOCUS       DAOPKU010000460         2456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_49400_length_2456_cov_5.267389, whole genome shotgun sequence.
ACCESSION   DAOPKU010000460 DAOPKU010000000
VERSION     DAOPKU010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49400_length_2456_cov_5.267389"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PK849_12220"
     CDS             <1..761
                     /locus_tag="PK849_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPE66937.1"
                     /translation="FTSDHGEMGGAHGLKGKGGLLFDRNIHVPLIVYHPDYEGGHFVN
                     AVTSHIDLATTFIHMSGLSAERKAEVAEGLPGKNLMELIDGTSQSVRRGALFCNELIS
                     TTMARAVRDVNGAIQYYTFDTTVRGLVRAAITERYKFARYFGGNFNTPTTMEALLRDN
                     DIELYDLENDPGELNNLALDPEANAELIMEMNALLNELIASEIGEDNGDEFHEMIAAY
                     NATLRKESGGCSTASVPAGLLLLLALLPLLRRRK"
     gene            766..1935
                     /locus_tag="PK849_12225"
     CDS             766..1935
                     /locus_tag="PK849_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HPE66938.1"
                     /translation="MTGNDSRTFAVMAKVVGGFCNLQCEYCYYTEKTKLLAQTRRLMP
                     DDVLEAYVVENLRIHGKDAVVEFAWHGGEPLLAGIDFFRRAMGLERKYGKGRKIVNTV
                     QTNATLLNDEWRQFFAKHDFLLGVSVDGPEALHDAYRRGPDGGSFSRAMAGIELLRKH
                     GIRFNTLTTVNAANEKHPEEVYDFLRRYTDYMQFLPVVESDAAFFEIEDGQRFATPPG
                     IRSMFLQHSMAPFSASAEGYGHFLAGVFDRWKELDYGRKHVQIFETTAGNMRGVPSSL
                     CVHNPLCGHGASVEVDGAVYSCDHYAFPNYMLGNILETPLDKIMEKNREFGMHKTYGL
                     PKECFACPYIKLCFGGCPKDRVLLSRDGERGKNYLCEGYRIFFKHFLEQMEGVLP"
     gene            2139..>2456
                     /locus_tag="PK849_12230"
     CDS             2139..>2456
                     /locus_tag="PK849_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPE66939.1"
                     /translation="MDKLSIGKMAEINRVSVQTLRLYDRLGLLKPQYVDENRYRYYSI
                     KQSARLDMIQNLQLLGMSLRQIREQLDTQDVAVVEKLLEKQKARLDEELLRLSATKRC
                     VERT"
BASE COUNT          543 a          711 c          764 g          438 t
ORIGIN      
        1 tcttcacctc cgaccacgga gagatgggtg gcgcgcacgg cttgaagggg aagggcggtc
       61 tgctcttcga tcgcaacatc cacgtcccgc tcatcgtcta ccacccggac tacgagggag
      121 ggcatttcgt gaacgcggtc accagccata tcgacctcgc gacgaccttc atccacatga
      181 gcggcttgag cgccgaacga aaggcggagg tcgccgaagg gctccccgga aagaatctga
      241 tggaactgat cgacggtacg tcgcagtcgg tgcggagagg cgcgctcttc tgcaacgagc
      301 tgatctccac gacgatggcg cgcgccgtca gggatgtgaa tggcgctatc caatactaca
      361 ccttcgacac gaccgtccgc ggtcttgtgc gcgcggcgat cacggagcgc tacaagtttg
      421 cccgctactt cggcggaaac ttcaacacgc cgaccacaat ggaagcgctg ctgcgcgata
      481 acgacatcga actgtacgat ctggagaacg atccgggcga gctgaacaac cttgctctcg
      541 acccggaggc gaacgcggag ctgatcatgg agatgaacgc gctgctgaac gagctgatcg
      601 catcggagat aggcgaggac aacggcgacg agttccacga gatgatcgcg gcgtacaacg
      661 caacgctgcg taaagaaagc ggcgggtgct ccacggcctc cgttccggcg ggacttctgc
      721 tcctgttggc gctgctgccg ctcctccgcc gcaggaagta aatagatgac gggaaacgat
      781 tcgaggacct tcgccgtgat ggccaaggtc gtcggcggtt tctgcaatct gcaatgcgag
      841 tactgctact acaccgaaaa gacgaagctc ctcgcgcaga cgcgccgcct gatgcccgac
      901 gacgtgcttg aggcatacgt cgtggaaaac ctgcgtatcc acgggaagga cgccgtcgtc
      961 gagttcgcct ggcacggcgg cgagcccctt cttgccggga tcgatttctt cagaagggcg
     1021 atgggcctgg aacgaaagta cggaaagggc aggaagatcg tcaacaccgt gcagaccaac
     1081 gcgacgctgc tgaacgacga gtggcgccaa ttcttcgcga agcacgactt tctcctgggg
     1141 gtcagcgtgg acggcccgga agcgctccac gacgcctacc ggaggggacc ggacggaggt
     1201 tcgttcagcc gcgcgatggc gggaatcgaa ctgcttcgga aacacggaat acgcttcaac
     1261 acgctgacga ccgtcaacgc ggcgaacgaa aaacatccgg aggaggtcta cgacttcctc
     1321 cgcagataca ccgactacat gcagtttctg cccgtggtcg agagcgacgc cgcttttttc
     1381 gagatcgagg acgggcagcg gttcgcgacg cctccgggga tcaggagcat gtttctgcaa
     1441 cactcgatgg cgccgttttc agcaagtgcg gaagggtacg ggcactttct tgccggcgtc
     1501 ttcgaccgat ggaaggagct ggactacggg aggaagcacg tgcagatttt cgagacgact
     1561 gccggcaaca tgcgcggcgt gcccagcagt ctgtgcgtcc acaaccccct gtgcgggcac
     1621 ggagcgtccg tcgaggtcga cggcgcggtc tattcgtgcg accactacgc tttcccgaac
     1681 tacatgctgg ggaatatcct cgaaacgccg ctcgacaaga tcatggagaa gaaccgtgaa
     1741 ttcggaatgc acaaaacgta cgggctcccc aaagagtgct tcgcctgccc gtacatcaag
     1801 ctctgcttcg gaggctgtcc gaaggacagg gtgctcctct cgcgggacgg agaacgggga
     1861 aagaactacc tctgcgaagg gtacaggatc tttttcaaac actttctgga gcagatggaa
     1921 ggggttcttc catagccgac tcggcgccgt tgttttttac ccggggagac ttcgcttcgc
     1981 cctatccgaa acgccgccgc tccgaagaga atgtggtata ttcttcggga gcggcggaac
     2041 tgcgtcgctc tccgtagact cgggggggaa acgtttccgg agcgggcggg agaaaagtcc
     2101 gcgaggtttt cagagagtgc ctgcaaaggg gtttttcgat ggacaagctc tcgataggca
     2161 agatggccga aatcaacagg gtctcggtgc agacgctgcg cctctacgac cgtctggggc
     2221 tgctgaaacc gcagtacgtg gacgagaacc gctacagata ctacagcatc aagcagtcgg
     2281 cccgtctgga catgatacag aaccttcagc tccttggcat gtcgcttcgt cagatcaggg
     2341 agcagctcga cacacaggac gtcgccgtcg tcgagaagct gctcgaaaag cagaaggcca
     2401 ggctcgacga ggaactgctt cgtctctccg ccacaaaaag atgcgtcgag cgcacg
//
LOCUS       DAOPKU010000461         2454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_49457_length_2454_cov_5.744894, whole genome shotgun sequence.
ACCESSION   DAOPKU010000461 DAOPKU010000000
VERSION     DAOPKU010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49457_length_2454_cov_5.744894"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PK849_12235"
     CDS             <1..729
                     /locus_tag="PK849_12235"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HPE66940.1"
                     /translation="YAYMYGVPYHYYEKYKVRRYGFHGTSHYYVSHRAAEILGKPVES
                     LNIVTCHMGNGSSLAAVKGGKSVDTSMGFSPLPGVIMGTRSGDVDPLLISYLSDQEGI
                     DNKAMSHVLNKESGLLGISGVSSDLRDIEEAADSGNQRAKLAEDMLYYGVKKYVASYA
                     AAMGGIDVLVFTAGIGENSARARESICAGLEFMGIKLDKEKNNVRGKERIVSTDDSRV
                     KVIVIPTDEELVIARDTKKIAIKN"
     gene            757..1377
                     /locus_tag="PK849_12240"
     CDS             757..1377
                     /locus_tag="PK849_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPE66941.1"
                     /translation="MPITEKPDSWRFEIDTPELGNEDAVTTALWEQEVLSRPGLGSAP
                     DSARAEILYDGLLFRFASPLKIEAEARWADDSLCVTIAVKAFFTTQCSRCLEPTDIEI
                     LDDFMYLYSLRKKTAANRNATEETDDEYQVVPVASWKNRLDISDQVWESLILTLPMQV
                     LCSRDCRGICPTCGKSLNSGECICSSETKDPRFEKLLAIQFDQDTE"
     gene            1396..1593
                     /gene="rpmF"
                     /locus_tag="PK849_12245"
     CDS             1396..1593
                     /gene="rpmF"
                     /locus_tag="PK849_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPE66942.1"
                     /translation="MATPKSRVSHARTHKRKSEWIRSLSGPELTTCPHCGEVVMNYTA
                     CGECGFYRGRKVLETKTEAKE"
     gene            1767..2336
                     /gene="fapR"
                     /locus_tag="PK849_12250"
     CDS             1767..2336
                     /gene="fapR"
                     /locus_tag="PK849_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HPE66943.1"
                     /translation="MKSQRKKNRQEKLLKLIEQNPLATDEQLAGILSASISTIRLDRA
                     VLAVPELRERMRSMAQKATSRLRSLKQSEVVGDLLELEPNKWALSVLETRREMAFRET
                     DMVWDHYIYAQASSIAIAVVEADFVIVDSMRGEYKGHARVGDALIARAKVGVRKDDKY
                     IVSVRTKVGDREIFVGRFIAAIVAENRLF"
BASE COUNT          647 a          581 c          669 g          557 t
ORIGIN      
        1 tatgcctata tgtacggcgt cccctaccat tactacgaga agtacaaagt gcgacgctac
       61 ggctttcacg gtaccagcca ctactatgtt tcccacagag ccgctgaaat tcttggcaaa
      121 cctgtcgagt cgctcaatat tgtcacatgc cacatgggga acggcagctc tcttgccgcg
      181 gtaaaaggcg ggaagtcggt cgatacaagc atgggattca gcccccttcc aggcgtcatt
      241 atggggactc gatccggcga cgttgacccg ctgctcatct cttacctttc agaccaggaa
      301 ggaatcgaca acaaggcgat gagtcacgtc ctgaacaagg aaagcgggct tcttggtatt
      361 tccggcgtca gcagcgatct ccgtgatata gaagaagcgg ccgattccgg aaaccagcgg
      421 gccaaacttg ccgaggacat gctctattac ggcgtcaaga aatacgttgc gtcgtatgct
      481 gccgccatgg gaggcattga tgtgcttgtc ttcaccgccg gcatagggga aaacagcgcc
      541 cgcgcccgcg agagcatctg cgccggcctt gaatttatgg gaatcaaatt ggacaaagag
      601 aaaaataacg tccgtggtaa agaacgcatt gtcagcaccg acgacagccg tgtaaaagta
      661 atcgtaatac cgacggacga agagctcgtt atagctcggg atactaagaa gatcgcgatt
      721 aaaaactagt cgctagagcc ctaggagggg aaggacgtgc caataacgga aaaaccggac
      781 tcatggcgtt ttgaaattga taccccggag cttgggaatg aggatgcggt taccacggcc
      841 ctttgggagc aggaagttct gtcccggccc ggacttggtt ccgcacccga ttcagcccgg
      901 gcggaaattc tttacgacgg gcttcttttc cgttttgctt ccccgctgaa gatagaagcg
      961 gaagcgcgtt gggcagacga tagtttgtgc gttacaattg ccgtaaaagc tttttttaca
     1021 acgcaatgtt ctcgatgtct tgagccgaca gatattgaaa ttctggatga tttcatgtat
     1081 ctttattctt tgcggaagaa gactgccgca aaccgtaacg ctacggagga aacggatgat
     1141 gagtatcaag tagtaccagt cgcttcctgg aaaaatcgtc tggacatttc ggaccaggtc
     1201 tgggaaagct tgattcttac tcttccgatg caggtgctct gctctaggga ttgccgcggc
     1261 atatgtccga cgtgcggaaa gtctctgaac tcgggagaat gtatttgctc ttccgaaaca
     1321 aaggacccgc ggtttgaaaa gcttcttgca atacaattcg atcaggatac agagtgaaaa
     1381 ggaggggaat gcagaatggc gacgccgaaa agcagggtat cccacgcccg tacacataaa
     1441 agaaagtcgg agtggatccg gtccttgtcc ggccctgagc tgaccacatg tccgcactgc
     1501 ggagaagtgg taatgaacta cacggcttgc ggagaatgcg gattttatag aggccgaaaa
     1561 gtactcgaaa ccaagacgga agcaaaagag tagcttgcag actatactca gaacggttta
     1621 gttgcaaaag agagagggga aaccctctct ctttttgttt catcttccat gcttcaccta
     1681 ttgaccgctc gctgtaaccc aattatactt accttgtctt gagagcagat gttatcatca
     1741 ggtggttatt gaggggggcg acttccatca agtctcaaag aaaaaaaaac cgacaggaga
     1801 agttgctgaa actcatcgaa caaaacccgc tcgcgacaga cgaacaactc gcaggcattc
     1861 tttccgcgag tatcagtact attcgtcttg acagggccgt tcttgccgtg ccggagcttc
     1921 gcgaaagaat gcgttctatg gcgcagaaag ccaccagtcg actgcgttct ttaaagcaaa
     1981 gcgaggttgt cggcgacctt cttgaactgg aacccaacaa atgggctctg tccgttttgg
     2041 aaacccgcag ggaaatggcg ttccgcgaaa cggacatggt atgggaccac tacatctatg
     2101 ctcaagcaag ttctattgcg attgcggtgg tagaagctga tttcgtaata gtggactcga
     2161 tgagaggcga atacaaagga cacgctcgcg tgggagatgc ccttatagcc agagcgaagg
     2221 taggagtccg caaggatgat aaatatatag tcagcgtacg gacgaaggtc ggagacagag
     2281 agatttttgt cggaaggttc atagccgcta tcgtcgcgga aaatcggctt ttttgagtct
     2341 ggagagaggt gaaccctgtt tgattcttgc ccttgacgca atgggaggcg accatggccc
     2401 ttccgaaata tgcaaggcgg cgctgctcgc ctgcgaagag tatagcgatc ttga
//
LOCUS       DAOPKU010000462         2452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_49533_length_2452_cov_4.531498, whole genome shotgun sequence.
ACCESSION   DAOPKU010000462 DAOPKU010000000
VERSION     DAOPKU010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49533_length_2452_cov_4.531498"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..418
                     /locus_tag="PK849_12255"
     CDS             158..418
                     /locus_tag="PK849_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPE66944.1"
                     /translation="MTNALHISVLAVMLIAAFFALWFKDLISSVISLAVFSLMMALEF
                     YILQAPDVAIAEAAIGAALSTTIYIIALRACRRSKSEGGRKQ"
     gene            424..687
                     /locus_tag="PK849_12260"
     CDS             424..687
                     /locus_tag="PK849_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66945.1"
                     /translation="MIFVVAALLLGAMVVGAVDNAHPFGFPGKTPMDDFFLADALPNR
                     STENIVTAIVFDYRGFDTFGEAAVLFTALCAISVLFREGGRKR"
     gene            684..1151
                     /locus_tag="PK849_12265"
     CDS             684..1151
                     /locus_tag="PK849_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HPE66946.1"
                     /translation="MNPLSVVVRTGCDLFAWFMVVFGVNVLVHGYMTPGGGFQGGAII
                     ATFTAFLLVAYGGRKLLGWANEKIFDLFEEVGLLAFFFLAFMGFPVTFFYNYLVKPLA
                     AGGVVPPAGTTALMSIAVGLEVSGALSMVILTMYRGIRMFETAPMEEEIGHDR"
     gene            1141..1488
                     /locus_tag="PK849_12270"
     CDS             1141..1488
                     /locus_tag="PK849_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPE66947.1"
                     /translation="MIGNAPFFVVGLLFLGGLTAIFIEKNLIKIAIAIGIISSAVNLF
                     LVALGYRAGGTAPIHFLAGERTVMVLPTPQAMTLTAIVISLATTAFLLSLIMLVYRHY
                     GTLDVDEIRRLRG"
     gene            1485..>2452
                     /locus_tag="PK849_12275"
     CDS             1485..>2452
                     /locus_tag="PK849_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPE66948.1"
                     /translation="MNWNDHLPALLLMIPLFGAFAAPLVCLLGKTMRNVLLVGLSFVT
                     LLVALLLWKSVLAGGIAVYVMGGESFNLTLPSGMALPIRIIMEVDAFSAFMVVCGSIA
                     SFAGALFSTNYMEKFTGLGRFVSLYFLLTLGMLGMMITGDFFNFFIFIEIASIASFGL
                     VAFWRDRPEAIEASLKYTLVSQVGSMIFLIAAGALYGKYNALNIAAIGSLLQFGVMEK
                     IALVFIIGTLAMKCGSFPMHMWLPDAYAEAPSGVSCLLVAVSQASLYGLMRVCFSLYG
                     VNLGGAFIPWMIISMGLASMFFGVTMAVVQHEIKRLIGYHSVSQVG"
BASE COUNT          424 a          664 c          727 g          637 t
ORIGIN      
        1 tcatcataca tacgcttgtg gcgatgatgg tgctgcttgt cacgaacgca acgggagcgc
       61 acgccatcgc gcgggcctct caccggagcg gattggcgcc tgcaaaggcc gttgtcgaca
      121 ggcttgagga gagctgttcc agaggaggtg cgtcccaatg acgaacgcgc tgcacatctc
      181 ggttctggcg gttatgctga ttgccgcgtt cttcgccctc tggttcaagg atctcatctc
      241 ttcggtgatt tccttggcgg tcttcagtct gatgatggcg ctggaattct acattctgca
      301 agcgcccgac gtcgcgatcg ccgaggcggc catcggagcg gctctgagta cgacgatcta
      361 tataatcgcg ctcagggcgt gtcgccgttc aaagagtgaa ggggggcgga aacagtgaaa
      421 aagatgattt tcgttgtcgc tgcgcttttg ctcggagcaa tggtcgtcgg cgccgtggac
      481 aatgcgcatc ctttcggctt cccggggaaa actccgatgg acgatttttt ccttgcggac
      541 gctctgccga atagatcgac cgagaacatc gtcacggcga tagtcttcga ttacagagga
      601 ttcgatactt tcggcgaagc cgctgtttta ttcaccgcgc tctgcgcaat ttcggttctt
      661 ttcagggaag gaggaaggaa acggtgaatc ccctttcggt tgtagtccgg acaggctgcg
      721 acctgttcgc atggtttatg gtcgtttttg gcgtcaacgt tctggtgcac gggtacatga
      781 ctcccggcgg aggcttccag ggaggggcga ttatcgcgac gttcaccgcc tttttgttgg
      841 tggcctacgg cgggcggaag ctgctgggct gggcgaacga gaagatattc gaccttttcg
      901 aagaggtcgg gttgcttgct ttcttctttc tggccttcat gggattcccc gtcacgttct
      961 tctacaacta tcttgtgaag ccccttgcag ccggaggcgt cgttccgccg gcaggcacga
     1021 cggcgctcat gtccatcgcg gtgggactcg aagtgagcgg cgctctttcg atggttattc
     1081 tcacgatgta cagggggatc cgcatgttcg agaccgcccc gatggaggag gagatcggtc
     1141 atgataggta acgctccttt ttttgtggta ggccttcttt tcctgggcgg actgacggcg
     1201 atctttatag aaaagaatct catcaaaatc gccatcgcaa tcggtatcat cagttccgct
     1261 gtcaacctgt tcctagtcgc tttggggtat cgtgccggag gaacggcgcc tattcatttt
     1321 ctcgcaggcg agcggacggt catggtgctt ccgacgcccc aggcgatgac gctcaccgcg
     1381 atcgtgattt cgcttgcgac gacggccttt cttctctcgc tcatcatgct ggtctaccgt
     1441 cattacggca cgcttgacgt ggacgaaatc aggaggttga gaggatgaac tggaacgatc
     1501 accttcccgc gctgttgttg atgattcctc tcttcggcgc tttcgccgcg cctttggtct
     1561 gtcttttggg aaagacgatg cgcaatgtgc tgttggtcgg tctttctttc gtgacgctgc
     1621 ttgtcgcgct tcttctctgg aaaagcgtgc tcgcgggggg aatcgcggtc tatgtaatgg
     1681 gaggggagag cttcaacctg acgcttccct ccggtatggc gcttccgatc cgcatcatca
     1741 tggaggtgga cgcgttcagc gccttcatgg tcgtctgcgg ttcgatcgcc tcgttcgccg
     1801 gcgccctctt ctcaacgaac tacatggaga agttcaccgg cttggggcgg tttgtctcgc
     1861 tctatttcct tcttacgctc ggtatgctcg gaatgatgat tacgggcgac tttttcaact
     1921 tcttcatctt catcgaaatc gcatccatcg cctcgttcgg attggtcgca ttctggcgcg
     1981 acagaccgga ggcgatcgag gcaagtctta aatacacgct tgtctcgcag gtggggtcga
     2041 tgatcttcct gatcgccgca ggcgctctgt acggcaagta caacgcgctc aacatagcag
     2101 cgataggcag tttgctccaa ttcggcgtca tggagaagat cgcgttggtt ttcatcatcg
     2161 gaacgcttgc gatgaagtgc ggttccttcc cgatgcacat gtggcttccc gacgcctacg
     2221 cggaagcgcc ctcgggcgtc tcctgcctcc tggtcgcggt cagtcaggcg tcgctctacg
     2281 gcctgatgcg cgtgtgcttc tcgctgtacg gcgtcaacct cggaggagcc ttcatcccgt
     2341 ggatgatcat ctcgatggga ctcgcttcca tgttcttcgg ggtgaccatg gccgtggtgc
     2401 agcacgagat aaagcgcctg ataggctacc attcggtctc gcaggtcgga ta
//
LOCUS       DAOPKU010000463         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_49714_length_2446_cov_3.730657, whole genome shotgun sequence.
ACCESSION   DAOPKU010000463 DAOPKU010000000
VERSION     DAOPKU010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49714_length_2446_cov_3.730657"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PK849_12280"
     CDS             complement(<1..1143)
                     /locus_tag="PK849_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE66949.1"
                     /translation="MNVKTSALETTKRDVVVIGGGPAGLAAAIAAKEAGCGDVLLIER
                     DRILGGILNQCIHDGFGLHTFKEALSGPEYAARYIDRFHELGIEAMEETIVLSLTPDR
                     VVEVSTRGTLRRIRAGAVILAMGCRERTRGAISIPGNRPSGVYTAGAAQNFMNLENIM
                     VGRKVCILGSGDIGLIMARRMTLEGASVEAVFEILPYSSGLPRNIQQCLNDYGIPLFL
                     NTTVTEIVGKGRLEGIRVASVGEDRAPIPDTERFIACDTLLLSVGLIPENELSRDAGV
                     AMDPVTGGAVVNDSFMTSVDGIFACGNVLHVHDLVDWVSVEAAEAGKFAAGYVRSGRE
                     ASSCRIPVRPGSGVRYTLPQSVSGERDCILSLRVAAPWRNRCIVVKD"
     gene            complement(1140..>2446)
                     /locus_tag="PK849_12285"
     CDS             complement(1140..>2446)
                     /locus_tag="PK849_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPE66950.1"
                     /translation="SLRAILDVKGNSMMDDLCRDLKVKIKRIGKLTVALDEEDEGTLQ
                     RLLEQGRANGVPGLEIIGKERMQAIQPGVEGYMALYSPTSSIISPYALTIGLAENALA
                     NGVNFHLGQQVVSVTPLAGKGWEVGTKSGERFTCTVLVNASGLFAADVCRMAGITEYA
                     IYPCRGEYYVLDKRLDGTLRALIYPAPKKNAPGLGIHLTPTVDGNILIGPSAEYLNDP
                     EDHSCTYDVMAELRREGKELLGEIRMTDYIRNFAGIRPKQTPPEVGGNKDFVIEDRKD
                     AKGFVNLVGIESPGLTSAPAIAEMVRGMVEKHIPLLPDPAFNPVRPGSPFFFSELPAD
                     ERAAMIAENPNYGEIICRCEKITKQEILDAINNPLGARTLVSIKYRARASMGHCQGGF
                     CVPRITRILRDEFGWNPEDFLKRGGSSPMFVGNVRPKGGVSR"
BASE COUNT          438 a          802 c          726 g          480 t
ORIGIN      
        1 atcctttact actatacacc gattgcgcca cggggcggcc accctgaggg aaagtataca
       61 gtcgcgttcc cccgagacgc tctgcggcag cgtatagcga acgcccgaac ccggcctcac
      121 cgggatacga caggacgacg cctcccgtcc gctgcgaacg tacccggccg caaacttccc
      181 tgcttccgcg gcctccacag agacccagtc gaccaggtcg tggacgtgga gcacgtttcc
      241 gcaggcgaag ataccgtcca cggacgtcat gaaggagtcg ttcaccacgg ctccccctgt
      301 gacgggatcc atcgcgacgc ccgcatcccg ggagagctcg ttctccggga tcagtccgac
      361 ggacaggagc agcgtatcgc aggcgatgaa gcgttccgta tcgggtatag gcgcgcggtc
      421 ctcaccgacg gaggcaaccc gtattccctc aaggcgcccc ttgccgacga tttccgtcac
      481 cgtggtattc aagaagaggg ggatgccgta gtcgttcagg cactgctgga tgttccgggg
      541 cagaccgctc gagtacggca gtatttcaaa gaccgcctcg acagaggccc cttccagcgt
      601 catccgccgc gccatgatca gtccgatatc tcccgaaccg agaatacaga ctttccgccc
      661 caccatgatg ttctccaggt tcatgaagtt ctgggcggcg cctgcggtgt acacgcccga
      721 cgggcggttt ccgggaatcg agatggcgcc cctggtccgc tcgcggcacc ccatcgcgag
      781 gataaccgct cccgcgcgga tgcggcgcag cgtgccgcgg gtgctcacct cgaccacccg
      841 gtcaggagtg agcgagagga cgatcgtctc ctccatcgcc tcgatgccga gttcgtggaa
      901 cctgtcgatg taccgcgccg catactccgg tcccgagagg gcttccttga acgtgtgcag
      961 cccgaacccg tcgtggatac actggttgag gataccgccg agaatccggt cgcgctcgat
     1021 caggagcacg tcgccgcacc ccgcctcttt cgccgcgatg gccgcggcaa ggcccgcagg
     1081 tcctccgccg ataaccacga cgtctctctt cgtcgtctcc agtgcgctcg tcttcacgtt
     1141 catcgggata cgcctccttt cggccggacg ttgccgacga acatgggcga ggagccgccc
     1201 cgcttcagaa agtcctcagg attccagccg aactcgtcgc gcaggatccg ggtaatgcgc
     1261 ggcacgcaga aaccgccctg gcagtgcccc atcgaggcgc gggcgcggta ctttatgctc
     1321 acgagcgtcc gtgcgccgag agggttattg atagcgtcga gaatctcctg cttcgtgatc
     1381 ttctcgcacc tgcagatgat ctcgccgtag ttggggtttt ccgcaatcat cgccgcacgc
     1441 tcatcggccg gcagttcaga gaagaagaac gggctgccgg gtcgcacagg gttgaacgca
     1501 gggtcgggaa ggagcgggat atgcttctcc accattcccc gcaccatttc ggcgatcgcg
     1561 ggggcgctcg tcaaaccggg gctttcaatg ccgacgagat tgacgaaccc cttcgcgtcc
     1621 tttctgtctt cgatgacgaa gtccttatta ccgccgactt cggggggcgt ctgcttcggg
     1681 cggataccgg cgaagttgcg gatgtagtcc gtcatgcgta tctcgcccag gagttccttc
     1741 ccctcgcggc gcagctccgc cataacgtcg tacgtgcagg agtggtcttc cggatcgttc
     1801 aggtattcgg cgctcgggcc gatgaggatg tttccgtcaa ctgtgggggt gagatgaatg
     1861 ccgagacccg gcgcgttctt ttttggcgcc gggtaaatga gcgcccggag ggttccgtcg
     1921 agccgtttgt cgaggacgta gtattcgccc cggcatgggt agatcgcgta ttcggtaatt
     1981 cctgccattc tgcagacatc cgccgcgaag agtccggaag cgttcaccaa aaccgtacat
     2041 gtgaaacgct cccccgactt cgtcccgacc tcccacccct ttccggcgag tggcgtgacc
     2101 gagacgacct gctgccccag gtgaaagttc accccgttgg ccagcgcgtt ttccgcaagg
     2161 ccgatcgtaa gcgcgtaggg cgagatgatc gagctggtgg gcgagtagag cgccatgtac
     2221 ccctccacgc ccggctgaat agcctgcatc cgctccttgc cgataatctc cagaccggga
     2281 acgccgtttg cccgcccctg ttcgagaaga cgctgaagcg ttccttcgtc ctcttcgtcg
     2341 agggcaaccg tcagcttgcc gatacgcttg atcttcacct tgagatcgcg gcagagatca
     2401 tccatcatcg agtttccctt cacgtcgaga atcgctcgca gcgatc
//
LOCUS       DAOPKU010000464         2441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_49852_length_2441_cov_4.304694, whole genome shotgun sequence.
ACCESSION   DAOPKU010000464 DAOPKU010000000
VERSION     DAOPKU010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49852_length_2441_cov_4.304694"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PK849_12290"
     CDS             <1..571
                     /locus_tag="PK849_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPE66951.1"
                     /translation="VMALIENIHRSNLSAIEEAASLRDILESTGWSQTELAARLGRSQ
                     SSVANKLRLLKLEEPVQLLVLQGKLGERQARALIALTGEEQIAMAHTAIEENLVAGEV
                     ERRVKEERESSPRTFAAPSAARKKEALSFTGPDGPTGEILKDIAELVEKSRKKGIPVV
                     WKVKELAQRELVLELVVDLRSVSRVSEID"
     gene            complement(684..>2441)
                     /locus_tag="PK849_12295"
     CDS             complement(684..>2441)
                     /locus_tag="PK849_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPE66952.1"
                     /translation="STFEARGYSAWDPSSPAFIMMSKKGGTLCIPSVFISYDGTPLDL
                     KTPLLKAVDAVETRAMRILKLFGNRGLKWAHVTVGAEQEYFLIDNAVAKDRLDLRYCG
                     RTILGCPPPKGQQMEDHYFGSIPSRVLAFMEDVERDLYRLGVVITTRHNEVAPCQFEF
                     APQFADANLACDQNQQIMDVMRKMAKRHDFALLLHEKPFAGLNGSGKHVNFSIMDSEG
                     RNLLKPSTNHRKNIQFLTFLSALLLGFGKYYGLIRASIASPGNMHRLGGNEAPPAIMS
                     AYLGSAITGILEAVEKGLPNDLPAQALIDLGMNKLPSIMADNSDRNRTAPLAFTGNKF
                     EFRAPGAPQSIAGPLTMLLAAWAWGIDRVADMIEAKGSDVDVLEAALDAIKYAAAESR
                     AIRFEGNAYSAEWQQEAKKRGLKIANSTQEALALYLVPEHRELLASLGIMSEREVVAY
                     YEVRLEQYIKTVSIEMGIMRAMLWEGILPAISRQILLEQGALSSLPEEMIKESVLWKK
                     SVANLVSIKNGLIAASEKLDAMLAKIHELEIDEQSRMISEEALPLYVHIRSLSDRAET
                     LIAKDIWPYPTYTRLLNIS"
BASE COUNT          518 a          637 c          727 g          559 t
ORIGIN      
        1 ggtgatggcg cttatcgaaa acatacaccg gagcaatctc tccgctatcg aggaggcggc
       61 gagccttcgc gacatccttg agagcacggg gtggagccag actgagctgg ctgccaggct
      121 tggacgctct cagtcctccg tggccaacaa gctgcgactg ttgaagctgg aggagccggt
      181 acaacttctc gttctgcaag gaaagctggg agaacgacag gcccgcgcgc tcatcgctct
      241 taccggtgag gaacagatag ccatggcgca caccgcaata gaagaaaatc tcgttgcggg
      301 agaggttgaa cggcgggtca aagaagaaag ggaatcctcg ccaaggacct ttgcagcacc
      361 ttctgcggcg agaaaaaagg aggccctttc ctttaccggg cctgatggtc cgacgggaga
      421 gatcctcaag gatattgcgg aactggtgga aaaaagcagg aaaaaaggaa ttcctgtggt
      481 ctggaaagta aaggagcttg cgcagcggga gctggtgctc gaactggtgg tggatcttcg
      541 cagcgtctct cgtgtttccg aaatcgactg agcagctctt tttccaatag aaacgaacag
      601 gagaagcgct ccgcgtgtca gatgcggagc gcttcgattt tttattcggg ggcgtatatg
      661 cggaacatgg tgtcgctgat tcgtcaggaa atgttcagga gtctggtgta cgtcgggtag
      721 ggccaaatat cctttgctat aagcgtctcc gctctgtctg aaaggctccg gatgtgcaca
      781 tacaggggaa gcgcctcctc gctgatcatg cgagactgtt catcgatttc gagttcgtgt
      841 atcttggcaa gcattgcgtc gagtttctcg gaagcggcga tcaacccgtt ctttattgaa
      901 accaggttgg caacgctctt cttccacaaa accgattcct tgatcatttc ttcgggtagc
      961 gaagagagcg ctccctgttc caggagtatc tgacgggata tcgccgggag tatcccctcc
     1021 cagagcatgg cccgcatgat tcccatttcg atggagactg tttttatgta ctgttcaagg
     1081 cgaacctcgt agtacgcgac aacctctcgt tcgctcatga tgccgaggct tgcgagcagt
     1141 tcccggtgtt cgggtacaag atagagcgcc agggcttcct gggtgctgtt cgctatctta
     1201 agtccccttt tcttcgcctc ctgctgccac tccgcgctgt aggcgttgcc ttcgaagcgg
     1261 attgcacggc tttcagccgc tgcatacttg atcgcgtcga gcgccgcttc aaggacgtcg
     1321 acgtcggaac cctttgcttc gatcatgtcg gcgaccctat cgattcccca tgcccaggct
     1381 gcaagaagca tcgtcagcgg accggcgatg gattgcgggg cgcccggcgc ccggaattca
     1441 aacttgttcc cggtgaaggc gagcggcgca gtcctgttgc ggtcggaatt atccgccatg
     1501 atggagggca gtttgttcat ccccagatcg atcagcgcct gcgcggggag atcgttcggc
     1561 agtcctttct caacggcttc caaaattccg gtaatagctg aaccaaggta tgcgctcatt
     1621 atggcgggcg gggcttcgtt tccgccgaga cggtgcatat ttcccggaga ggcgatcgaa
     1681 gcccggataa ggccgtagta cttcccgaac ccaagaagga gagcgcttaa aaaagtgagg
     1741 aactggatgt tcttcctatg gttcgtcgac ggtttcagaa ggttgcgccc ttcactgtcc
     1801 ataatagaga aattgacatg tttcccgctg ccgttgagcc ctgcgaacgg cttttcgtga
     1861 agtagaagcg cgaaatcatg acgctttgcc atctttcgca tgacgtccat gatctgctgg
     1921 ttctggtcgc aggcaaggtt cgcatcggcg aactgcggag cgaactcgaa ctggcacgga
     1981 gcgacctcgt tatgccgggt ggtgatgacg acgcccagcc tgtagagatc ccgctctaca
     2041 tcttccatga aggcgagaac gcgtgaaggg atggagccga agtagtggtc ttccatctgc
     2101 tgccccttgg gcggaggaca gcccaggatt gtgcggccgc agtagcgcag gtcgagacga
     2161 tccttggcga cggcattgtc gataagaaag tactcctgtt cggcgccgac cgttacgtgc
     2221 gcccatttga ggccgcggtt tccgaaaagc ttcaaaattc gcatcgcgcg ggtttccact
     2281 gcgtctaccg ctttgaggag cggcgtcttg aggtcgagag gggttccgtc ataagaaata
     2341 aaaacggagg gaatgcagag tgtaccgcct tttttgctca tcatgatgaa tgccgggctt
     2401 gaaggatccc atgcgctgta tccgcgggct tcgaacgttg a
//
LOCUS       DAOPKU010000465         2439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_49893_length_2439_cov_5.651846, whole genome shotgun sequence.
ACCESSION   DAOPKU010000465 DAOPKU010000000
VERSION     DAOPKU010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49893_length_2439_cov_5.651846"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PK849_12300"
     CDS             complement(<1..391)
                     /locus_tag="PK849_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE66953.1"
                     /translation="MLFIVFLILLFAGLPIAFCLGISSLVYLWMAGIPLIVIPQKMYS
                     GIDVFVLLCIPGFILAGNLMNRGGISDRIIRFCNALVGHIQGGLAQANIVASMIFAGI
                     SGTAVADTASLGAILIPAMKKEGYDAEF"
     gene            complement(402..881)
                     /locus_tag="PK849_12305"
     CDS             complement(402..881)
                     /locus_tag="PK849_12305"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE66954.1"
                     /translation="MKKPFNLFTRIMEFCVIVTFLGLVAVVLLQVYARLFLPRSPHWT
                     EEASRFLFAYTVSFAAGLAVKEKAYVNVDVFTNLLPEMLKQWLEVLLDLLVAAFMAVV
                     FYYSLKNLGIGRMRTSPSLTIPMHYIFASMSVMSGSIVLYSLAEVIRDTRALLKGGN"
     gene            complement(960..1943)
                     /locus_tag="PK849_12310"
     CDS             complement(960..1943)
                     /locus_tag="PK849_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPE66955.1"
                     /translation="MKKIALLIAVALLSSLVFSTAALAEKKPEFTLKLGHLANTEHTW
                     HKGSVKFAELVEEKTGGRVVVQVFPSEQLGNEMEVIGNIQSGIVDMLITGESMMNWAP
                     LCGLIAVPYMIRDSAHMEKIVEGEVGQAIEKEVIEKVRLRPVAWFERGARNLTSNREV
                     KKPEDLNGLRLRVPNVPLFVATWESLGAKPTPMAFSEVFTSLQQGIIDGQENPYDLIK
                     SASFFEVQKFVNKTEHVRGWIYLVIGEDKFQSMPEDIRKAVLEAGKEAQVYERQLFRA
                     EQDQLIKDLQDKGMTFVEVDKEAFMKKAEPAVLSFLSPELQEMYKKIVAIQ"
     gene            complement(2185..>2439)
                     /locus_tag="PK849_12315"
     CDS             complement(2185..>2439)
                     /locus_tag="PK849_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66956.1"
                     /translation="ELACEEFSSESMVALRANLQLQRLAISEKNASRFFALDEDFHRI
                     IFDLREGAHLGGDPEDGVSFQPGSRAQSAGDGLGKSVFSA"
BASE COUNT          581 a          666 c          673 g          519 t
ORIGIN      
        1 aaaactccgc gtcgtacccc tcttttttca tcgcggggat gaggatggcg ccaagacttg
       61 cggtatcagc gacagcggtc cccgaaattc cggcgaagat catcgaagcc acgatgttgg
      121 cctgggcgag tccgccctgg atgtgcccga cgagcgcgtt acagaagcgg atgatccggt
      181 cggagatacc gccgcggttc atcaggttcc ccgcgaggat gaatcccggg atacagagca
      241 gtacgaagac atcgatcccc gagtacatct tctgcggaat cacaatgagc ggaatgcctg
      301 ccatccagag atagaccaga gaagagatac caaggcagaa agcgatgggg agccccgcaa
      361 agagaagaat gagaaagacg ataaaaagca tcgcgaccac gtcagttgcc ccccttcaat
      421 agagcacgag tatcccgaat gacttccgcc agcgagtaga gcactatgga gcccgacatg
      481 acggacatgg aggcgaatat atagtgcatg gggatggtca gggagggcga cgtccgcatc
      541 ctgcctattc cgaggttttt cagcgagtag taaaaaacga cagccataaa ggccgcgaca
      601 agaaggtcca gcaggacttc aagccactgt ttcaacatct ccggaagtaa attggtgaag
      661 acatccacgt tcacatacgc tttttccttt acggccaatc ccgcagcgaa gctcactgtg
      721 tacgcgaaca aaaaacggga agcctcctcc gtccagtggg gagagcgggg aagaaagagg
      781 cgggcgtaca cctggagaag aaccacggcc acaaggccga gaaaagtgac gattacacag
      841 aactccatga tacgggtgaa gagattgaat ggttttttca tatgacggtc gccctcgtct
      901 ctcaaaaaaa gaaaaaggaa tccgggctcc cggaagactg gggcccggaa gaaatagtgc
      961 tactggatag cgacgatctt cttgtacatc tcctgaagtt cgggagagag gaagctgagc
     1021 actgcgggct cggccttctt cataaaggct tccttgtcga cttcgacaaa ggtcatcccc
     1081 ttgtcctgca gatccttgat cagctgatcc tgctcggcgc ggaaaagctg gcgctcatag
     1141 acctgcgctt ccttacccgc ttcaagaaca gctttccgga tatcttcggg catgctctgg
     1201 aacttgtcct caccgattac gagatagatc catccgcgga cgtgctcagt cttgttgaca
     1261 aacttctgga cctcgaagaa gctggcgctc ttgatcaggt cgtaggggtt ctcctgaccg
     1321 tcgatgatcc cctgctgcaa cgaggtgaac acttcggaga acgccatggg agtgggcttg
     1381 gcgccgagag actcccaggt agccacgaaa agagggacgt tcgggacgcg aagtctcagg
     1441 ccgttcaggt cttcgggttt tttcacttcg cggtttgaag tcaggttccg ggcgccgcgc
     1501 tcgaaccacg ccacgggacg aaggcggact ttctcgatga cttccttttc gattgcctgg
     1561 cctacctcgc cctccacgat cttctccatg tgcgcagagt cgcggatcat gtacgggacg
     1621 gcgataagtc cgcacagagg agcccagttc atcatggact ctccggtaat gagcatgtcg
     1681 acgatgccgg actggatatt cccgatgact tccatttcgt ttccaagctg ctcgctgggg
     1741 aaaacctgga cgacaacgcg gccgccggtc ttttcctcga caagttcggc gaacttgacg
     1801 gatcccttgt gccatgtgtg ctcggtgttg gcaaggtggc cgagtttcaa agtgaactca
     1861 ggtttcttct ccgccagtgc ggcggtcgag aaaacgaggg acgacagaag cgcaactgct
     1921 attaagagtg cgattttttt catcggaaca atctcctttc atcaatacga taccaggtct
     1981 tcccccaaac gaaccggggg cgattcacgc cgcgaaacaa atcgtacgct gccggccggc
     2041 gctcacctcc ctttttcggt tttgaaatag gacgggtact cctgtcggag tcttccgagg
     2101 tcgtaattca ccttcgtgag atgttcattc atcgcgtcgc gcgcttcacc gatttttttt
     2161 tcgaaaatcg aggaggcgat gcgttcatgc tgagaaaaca ctttttccca gtccgtcgcc
     2221 cgcagactga gcacgcgaac ccggttgaaa tgagacccca tcttccggat cgcctcccag
     2281 atgcgctcct tcccgcaggt cgaagatgat gcggtggaaa tcttcatcga gggcgaaaaa
     2341 ccgtgaagcg ttcttctcgc ttatagccag gcgttggagc tgaagattcg cccgaagcgc
     2401 gaccatgctt tcggatgaaa actcttcgca tgcaagctc
//
LOCUS       DAOPKU010000466         2433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_50090_length_2433_cov_4.026493, whole genome shotgun sequence.
ACCESSION   DAOPKU010000466 DAOPKU010000000
VERSION     DAOPKU010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50090_length_2433_cov_4.026493"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PK849_12320"
     CDS             <1..572
                     /locus_tag="PK849_12320"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPE66957.1"
                     /translation="GNTFRYLTMILERGKSLGAETELVQLADKNLRGCRGCYECIAAK
                     RCTLDDDFQGIFPKMAQADAILLGSPVYHSSITAELKAVLDRAGFSGRWAKNEMKKEG
                     ESYTWGTTALSGKVVVPVSTARRAGHNFAFAQMLLWAAANDCIIVGNTYWNVGVAGKG
                     GAKNAEEDEEGVGIMTNIADRVVALLKRL"
     gene            586..2148
                     /locus_tag="PK849_12325"
     CDS             586..2148
                     /locus_tag="PK849_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPE66958.1"
                     /translation="MPNSWIGNYSFFRARIDSSREAVYDLDTETRYTYGDLERRANLL
                     ARYLKDQAGVRKGDRVAFIARNCIEMIDAYYATGKLGAIFVPYNARLSVSELVQLVNN
                     ETPKVLFYEEVFADKIPHLKSGCSVEKYLVLSPGKNPENDATYAEATGHGNDAFVGCD
                     ELDIEDIHMIIHTGGTTGLPKGGLISHRCELFNCLNEICTWNLHHEDSTLLLLPLFHT
                     GGWNLLTLPLLHVGGRLVINRQFDPKKTLEIVEKERITTLFGAATIFRMMVELPEFAS
                     ADFSSVKWIMAGAAPTPVNIMEKFWNKGVKFVLGYGMTEAGPNNISSCAHLISDDVMK
                     AKYASVGKPMYLSMVKLADDDGNEISEPNTPGELLWGGPQTFSGYWNNPDETAKTLKD
                     GWVHSGDMAVRDEDGYYYIVGRKKNMFISGGENVFPPEIERAMYDIAEIHEVCVFGVP
                     DEKWGEVGKAVVSLKPGKRVSKEEIVAALGSKLARYKIPKYIAFVDEVPKNNVGKIVV
                     SKVVELYGRATD"
     gene            2149..>2433
                     /locus_tag="PK849_12330"
     CDS             2149..>2433
                     /locus_tag="PK849_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66959.1"
                     /translation="MGVTLLKSADLVGIRSIGSYVPSGIRGGAYIAAASGIPEKVIRE
                     KFGINLVHKAPPEESVSAMGAKAALRALEGIDPATLDLVVYCGSEYKDYYL"
BASE COUNT          503 a          728 c          811 g          391 t
ORIGIN      
        1 acggcaacac cttccgctac ctgaccatga ttctggagcg cggaaaaagt ctcggcgcgg
       61 agacggagct ggtccagctc gccgacaaga acctcagggg gtgcagaggg tgctacgagt
      121 gcatcgccgc gaagcgctgc actctggacg acgacttcca gggaatcttc ccgaaaatgg
      181 cgcaagccga cgctatcctg ctcggctcgc ccgtctacca ctcttcgatc actgcggagc
      241 tgaaggcggt tctcgacagg gccggcttct ccggccgctg ggcgaagaac gagatgaaaa
      301 aggaaggcga gagctatacc tggggaacga ccgcgctctc cgggaaggtg gtcgtgccgg
      361 tgagcaccgc ccgcagggcg gggcacaact tcgccttcgc gcagatgctg ctgtgggccg
      421 cggcgaacga ctgcatcatc gtcggcaaca cctactggaa cgtgggggtc gccgggaagg
      481 gcggcgcgaa gaacgccgag gaggacgaag agggcgtcgg catcatgacg aatatcgcgg
      541 acagggtcgt cgccctgctg aagcgcctgt aagggggggc tgaatatgcc gaacagctgg
      601 ataggcaact actcgttctt ccgcgcccgc atcgacagtt cgcgggaggc ggtctacgac
      661 ctcgacacgg agacgcgcta cacctacggc gacctcgaac ggcgggcgaa cctgctggcg
      721 cgctacttaa aagatcaggc cggagtgcgc aagggcgacc gcgtcgcctt catcgcgagg
      781 aactgcatcg agatgatcga cgcgtactac gctacgggga agctgggcgc gatcttcgtt
      841 ccgtacaacg cgcgcctctc cgtgagcgag ctggtgcagc tcgtcaacaa cgaaacgccg
      901 aaggtcctct tctacgagga ggtcttcgcg gacaagatac cgcatctgaa gtcgggctgc
      961 tccgtggaga agtacctcgt cctctccccc gggaagaacc cggagaacga cgcgacctac
     1021 gccgaggcga cggggcacgg gaacgacgcg ttcgtcggct gcgacgagct tgatatcgaa
     1081 gatatccaca tgattatcca caccggcggg acgacgggcc tccccaaggg cgggctgatc
     1141 tcgcaccgct gcgagctgtt caactgcctc aacgagatct gtacctggaa cctgcaccac
     1201 gaggacagca cgctgctgct gcttcccctc ttccacaccg gcgggtggaa cctgctgacc
     1261 ctgccgctgc ttcacgtcgg cggacggctg gtcatcaaca ggcagttcga tccgaagaag
     1321 acgctcgaga tcgtcgaaaa ggaacggatc accacgctct tcggcgcggc cacgatcttc
     1381 aggatgatgg tggagctgcc ggagttcgcc tccgcggact tctccagcgt gaagtggatc
     1441 atggcggggg ccgccccgac gcccgtcaac atcatggaga agttctggaa caagggcgtg
     1501 aagttcgtcc ttggatacgg catgacggag gccggcccga acaacatctc ctcgtgcgcg
     1561 cacctcatat ccgacgacgt catgaaggcg aagtacgcct cggtgggcaa gcccatgtac
     1621 ctttcgatgg tgaagctcgc ggacgacgac gggaacgaga tctccgagcc gaacaccccc
     1681 ggcgagctgc tgtggggcgg gccgcagacc ttctccggct actggaacaa cccggacgag
     1741 acggcgaaga cgctgaagga cggctgggtg cattcggggg acatggcggt gcgcgacgag
     1801 gatggctact actacatcgt cggccggaag aagaacatgt tcatcagcgg cggcgagaac
     1861 gtctttccgc cggagatcga gcgggcgatg tacgacatcg cggagatcca cgaggtctgc
     1921 gtctttggcg ttcccgacga gaagtggggc gaggtgggca aggccgtcgt ctcgctgaag
     1981 ccagggaagc gcgtctcgaa agaggagatc gtcgcggcgc tcggctcgaa gctcgcccgc
     2041 tacaagatac cgaagtacat cgccttcgtg gacgaggtgc ccaagaacaa cgtgggcaag
     2101 atcgtcgttt cgaaggtggt ggagctctac ggcagggcga cggactagat gggagtgacg
     2161 cttttgaaat ccgctgattt agtcggcata cgcagcatag ggagctacgt cccctccggc
     2221 atccgggggg gcgcgtacat cgctgcagcc tcggggattc ccgagaaggt catccgggag
     2281 aagttcggca tcaacctggt gcacaaggcc ccgccggagg agtccgtctc cgcgatgggc
     2341 gcgaaggccg cgctgagggc gctggagggg atcgaccccg ccacgctcga cctggtggtc
     2401 tactgcggca gcgagtacaa ggactactac ctc
//
LOCUS       DAOPKU010000467         2432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_50136_length_2432_cov_2.454775, whole genome shotgun sequence.
ACCESSION   DAOPKU010000467 DAOPKU010000000
VERSION     DAOPKU010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50136_length_2432_cov_2.454775"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PK849_12335"
     CDS             <1..1276
                     /locus_tag="PK849_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPE66960.1"
                     /translation="GGAISAILLSTPGTPAAAATAFDGYPMARQGKGGTALTVATWAS
                     FWGGILSTFALLLMAPALAKFSLRFGPPEYFVLAIMGLSSIVTLTKGSMVKGLLSGFL
                     GLTIATVGMDPLSGFMRFTFGQMDLFDGVPFMPALIGLFSISQILDLTAETRITENID
                     DATLSSIKRSKLPKGLAGTICRGGVIGTIVGMLPGAGATISSFISYNFAKQSAKDPET
                     FGKGNPKGVAAAESANNGCVGGSLIPLLTLGIPGNSVAAALMGGLMIQGLIPGPELFS
                     RYGTLTYGFILSLFIANIVFLVLGLYLAPYFARVTSTPNALLIPGIAILSVIGSYAIN
                     NNMFDVWLMIAFGIAGYFLEKGGFSTGALVLGLILGPIAELGFGQSLIMSQGSPMIFF
                     DRPLCILLWVITLLLMIPAFSSSLKKTKNRTE"
     gene            1733..>2432
                     /locus_tag="PK849_12340"
     CDS             1733..>2432
                     /locus_tag="PK849_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPE66961.1"
                     /translation="MDLQKKFLASVDKLFERKQFKSLKELLSTMEPADIAEILAEKAA
                     AERAFLFRLLPKDLAIEVFEFMEGTEREELLANFTDSEVASIIEEMSDDDRTALFDEL
                     PAKTVKKLLAHLSPEERKLANALLNYPEDSAGRIMTPEFIDLKEGMTVARALERIRAT
                     AAKKETIYTSFVVSPERRLKGTVHLEDLILAAPETLLTDIMDGQPVFVTTSTDQEEAA
                     KIMSRYDLQTLPVVD"
BASE COUNT          484 a          780 c          672 g          496 t
ORIGIN      
        1 cggcggcgcg atctccgcca ttcttctctc gacgcccgga actccggcgg cggcggcaac
       61 cgctttcgac gggtacccca tggcgcggca agggaaaggc ggtacggcgc tcacggtcgc
      121 aacgtgggcc agtttctggg gtggcatact ctccaccttc gcccttctgc tcatggcgcc
      181 cgccctcgcc aagttctccc ttcgcttcgg acctcccgag tacttcgtcc tcgccatcat
      241 ggggctctcc agcatcgtca cgctgacgaa aggaagcatg gtcaaggggc tgctctccgg
      301 gttcctgggt ctcaccatcg cgacagtggg catggatccg ctctccggat tcatgcgctt
      361 taccttcggt cagatggacc ttttcgacgg cgttcccttt atgcctgcgc tcatcgggct
      421 cttctcaatc agtcagattc tcgatctgac agcggagacc cgcatcaccg aaaatatcga
      481 cgacgcgacg ctctccagca tcaaacggag caagctgccg aaaggactgg cggggacaat
      541 ctgcagaggc ggcgtcatcg gcaccatcgt cggcatgctg cccggcgccg gggcgaccat
      601 ctcctcgttc atctcgtaca acttcgcgaa acagagcgcg aaggacccgg agacgttcgg
      661 aaaggggaat cccaagggag tggccgccgc ggaaagcgcg aacaacggct gcgtgggagg
      721 ctcgctcatc ccgctgctca ccctcggcat tccgggaaac agcgtcgccg ccgccctgat
      781 gggcggtctg atgatccagg ggctcatccc gggccccgag cttttttcac gctacggcac
      841 gctgacctac ggattcatcc tctccctctt catcgcaaat atcgtcttcc tggtcctggg
      901 gctctacctg gcgccctact tcgcccgcgt cacgtcgacg ccgaacgcgc tgctcattcc
      961 gggaatcgcc attctctctg tcatcggcag ctacgccatc aacaacaaca tgttcgacgt
     1021 ctggctgatg atcgcgttcg gcatcgccgg gtacttcctt gaaaaaggag gcttttctac
     1081 cggcgcgctc gtgctggggc tcatcctcgg ccccatcgcg gagctcggat tcggccagtc
     1141 gctcatcatg tcgcaagggt ctccgatgat cttctttgac cgccccctct gtattcttct
     1201 ctgggtgatc accctgcttc ttatgattcc cgctttttcg agcagtctga aaaagacaaa
     1261 aaacaggacc gagtaaaaaa ccgacccttc ggggtcggtt tttttctccg ctgcgcctac
     1321 ggcgcacacg cgctcgcgag cgccgacaga agagcgcccg cgctgttttt gaatgctgta
     1381 ttcgctccgg cgaagaaaga aaacttgtca caaatggaag ccagtccggc tcgacatttt
     1441 ttccgttttg tggtactttt ttagcattat tattttccgc tgcctgaaaa ggagatggtt
     1501 cggtggatag cggagcgcaa cctttaagta gcactcttct cgcgtagtac ggcgccgcag
     1561 cggccttcct ttccggtaag ccgaagcccg gcgcccgccg ggattttcat taccggagat
     1621 ccctaacgaa atgcactcgg aacgaatacc gcatactccg cttttcggga aattgtctcc
     1681 ggaaggagat gatgccgtgt tgtgacgttc aggggacaag gaggcgtcgt gtatggatct
     1741 gcagaaaaaa ttcctcgcca gcgtggacaa actcttcgaa cggaagcagt tcaaaagtct
     1801 taaggagctc ctgagcacga tggaacccgc cgatatcgcc gagatcctcg cggagaaggc
     1861 ggccgcggag cgcgcgtttc tgttccggct tctgccgaag gacctcgcga tcgaggtctt
     1921 cgagttcatg gagggaaccg agcgcgaaga gctgctcgcg aacttcaccg actcggaagt
     1981 ggcctcgatc atcgaggaga tgtccgacga cgaccgcacc gcgctcttcg acgagctccc
     2041 cgcgaagacg gtgaaaaaac tgctcgcgca cctctccccc gaggaacgga agctggccaa
     2101 cgcgctgctg aactatccgg aggactccgc aggacgcatc atgaccccgg agttcatcga
     2161 cctgaaggag gggatgaccg tcgccagagc gctggagcgt atccgcgcca cggccgcgaa
     2221 gaaagagacc atttacacgt ccttcgtagt gagccccgaa cgccgcctca aggggacagt
     2281 ccacctcgaa gacctcatcc tcgcagcccc ggagacgctc ctgaccgaca tcatggacgg
     2341 acagcccgtc ttcgtcacca cctcgaccga ccaggaagaa gccgccaaaa tcatgtcccg
     2401 ctacgatctc caaacacttc ccgtcgtcga ta
//
LOCUS       DAOPKU010000468         2426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_50295_length_2426_cov_3.858709, whole genome shotgun sequence.
ACCESSION   DAOPKU010000468 DAOPKU010000000
VERSION     DAOPKU010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50295_length_2426_cov_3.858709"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK849_12345"
     CDS             complement(<1..448)
                     /locus_tag="PK849_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE66962.1"
                     /translation="MRIMKAVLLGVCAVLLVSSLAFGAETIKMGLLVPLTGPAAADGT
                     SALYSVQIAMDQVNKDGGVLGKQIELVYYDDRADAKEAVALSYKLIEQDKIAAFVAGS
                     YSLPTRAVAPIFQEEQIPLVAAYAIHPDVPTAGDFCFRNGFLGFVEG"
     gene            complement(554..1264)
                     /locus_tag="PK849_12350"
     CDS             complement(554..1264)
                     /locus_tag="PK849_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66963.1"
                     /translation="MLLEISDLSVFYGDIRAVDGLSLSLKEGELVSVIGANGAGKSSL
                     LNCIMGIVPRKNGSISFSGTDISSLPVHKRAAKGIRFVPERARVFPRLSVYENILTGA
                     YGIRSKIDFPGKLQWLYSLFPVLEERRNQLASTLSGGEQQMLAISRALIAEPKLLLVD
                     EVSMGLMPVLVDTVFDVLKMLNREHGLSILLVEQNALASLGISHRSYVLETGRIVLEG
                     ASEELLKDPKVQEAYLGL"
     gene            complement(1254..2021)
                     /locus_tag="PK849_12355"
     CDS             complement(1254..2021)
                     /locus_tag="PK849_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE66964.1"
                     /translation="MDDMLLRLDNVSKHFGGLKAVDEVSFSLGRGEILGLIGPNGAGK
                     TTCFNLVSGVYAPTSGKIFFEGASTGGFPPHRMAALGVGRTFQVVKPFSTLSVLRNVM
                     VALGLRRYGGVLSSFARWETPAVRKEATSLLEEVGIADLAEAPSGKLPLGNQRRLELA
                     RALALKPKLLLLDETFSGLRHEEVALLSRLVLDVRSKGIAVLLIEHNMKVAMGLSDRL
                     VVLDHGRLLAEGSAEDVRSNPRVVEAYLGKGASSHAS"
     gene            complement(2014..>2426)
                     /locus_tag="PK849_12360"
     CDS             complement(2014..>2426)
                     /locus_tag="PK849_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE66965.1"
                     /translation="ACFALREEETAASSMGVSPVRFKLMAFVIGTAMAGLGGALYAHQ
                     MRFISATDFGFPLSVMMMSMVVLGGIGTLWGPVLGALILGVLPEVFRPLVDYRMLLNA
                     AILLLMIRFQPDGLLGEGSLLRRIFPKRGETSHG"
BASE COUNT          465 a          798 c          783 g          380 t
ORIGIN      
        1 tgccctcgac gaagccgagg aaaccgttcc ggaagcagaa gtcgccggcc gtgggcacgt
       61 ccgggtgaat ggcgtacgcc gccaccagag ggatctgctc ctcctggaag atgggggcca
      121 cggcgcgggt gggaaggctg tacgagcccg ccacgaaggc cgctatcttg tcctgctcga
      181 taagcttgta ggagagggcg accgcttcct tggcgtcggc cctgtcgtcg tagtacacca
      241 gctcgatctg tttgccgagc acgccgccgt ccttgttcac ctggtccatg gctatctgga
      301 cggagtagag ggcgctcgtg ccgtccgccg ccgcaggtcc agtcaggggc acgagaagcc
      361 ccatcttgat cgtctccgcg ccgaaggcca gggacgaaac caatagtacc gcacacacgc
      421 cgagaagcac cgctttcata atccgcattc tctttccacc tccataagaa atttgcactg
      481 cctttgaaaa accgttccgc cgcgttctat ggcgccgcct ccgggaaaat cccggaggat
      541 acatccgaat cggttacagc cccaggtacg cctcctgcac cttcggatcc ttcagcagct
      601 cttccgaggc cccttcgagg acaatgcgcc ccgtctccag cacgtaggag cggtgggaga
      661 tgccgagcga ggccagcgcg ttctgctcca ccaggaggat tgagaggccg tgttcgcggt
      721 tcagcatctt cagcacgtcg aagaccgtgt ccacaagcac cggcatcagc cccatggaga
      781 cctcgtccac aagcagcagc ttcggctccg cgatcagcgc cctggagatt gcgagcatct
      841 gctgctcccc gccggagagc gtggaggcga gctggttcct gcgctcttca agcaccggaa
      901 agagcgagta gagccattgc agctttccgg ggaagtcgat cttcgaccgg ataccgtacg
      961 ctccggtgag gatgttctcg tagaccgaga ggcgcggaaa gacccgcgcc cgctccggga
     1021 cgaagcggat gcccttcgcc gcgcgtttgt gcacgggcag ggaggagatg tccgtacccg
     1081 agaaggaaat cgagccgttt ttgcgaggca cgatccccat gatgcagttc agcagggagc
     1141 tcttccccgc gccgttggcc ccgatgaccg agaccagctc cccctccttc agcgagagag
     1201 agagaccgtc gacggcgcgg atgtccccgt agaagacgga gaggtccgaa atctcaagaa
     1261 gcatgggagg acgccccctt tccgagatag gcctcgacca cgcgggggtt cgaccggacg
     1321 tcctccgcgc tcccctcggc gagcagtctg ccgtggtcga gcaccacgag gcggtcggag
     1381 agccccatgg cgaccttcat gttgtgctcg atgaggagca ccgcgatccc cttgctccgg
     1441 acgtcgagca caagccgcga gaggagcgcg acctcttcgt ggcgcaggcc ggagaaggtc
     1501 tcgtcgagca gcaggagctt cggcttcagc gcgagcgcac gtgcgagttc gaggcggcgc
     1561 tggttcccca gcggcagctt ccccgagggc gcctccgcca ggtcagcgat gccgacctcc
     1621 tcaagaagcg acgtcgcctc cttccgcacc gccggcgtct cccaccgggc gaatgacgag
     1681 aggacgccgc cgtagcggcg cagccccagg gccaccatga cgttccgcaa taccgagagg
     1741 gtggagaagg gcttgaccac ttgaaaggtg cgcccgacgc cgagcgcggc catcctgtgc
     1801 ggggggaatc ccccggtgga agccccctcg aagaagatct ttccggaagt aggggcgtac
     1861 acccccgaga cgaggttgaa acaggtggtc ttccccgcgc cgttggggcc gatgaggccg
     1921 aggatctccc ccctgccgag ggagaaggag acctcgtcga ccgccttcag gccgccgaag
     1981 tgtttcgaga cgttgtcgag gcgaagcagc atatcatcca tgagacgttt cccccctctt
     2041 cgggaagatg cgccgcagga ggctcccctc gccgagcagc ccgtcgggct ggaagcggat
     2101 catcagcagc aggatcgccg cgttgagcag catccggtag tcgacgagcg gacggaagac
     2161 ctcggggagc accccgagga tgagcgcgcc gagcaccggc ccccagaggg tgccgatgcc
     2221 gccgagcaca accatggaca tcatcatgac ggagagcgga aagccgaagt ccgtcgcgct
     2281 gatgaagcgc atctggtgcg cgtagagcgc gccgcccagg cccgccatcg ccgtaccgat
     2341 cacgaaggcc atcagcttga agcgcacggg ggagaccccc atgctggagg ccgccgtctc
     2401 ctcctcgcgg agggcgaagc aggcca
//
LOCUS       DAOPKU010000469         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_50486_length_2420_cov_4.230021, whole genome shotgun sequence.
ACCESSION   DAOPKU010000469 DAOPKU010000000
VERSION     DAOPKU010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50486_length_2420_cov_4.230021"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..358)
                     /locus_tag="PK849_12365"
     CDS             complement(89..358)
                     /locus_tag="PK849_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HPE66966.1"
                     /translation="MVGSWRVVFTKAALKDAEKLLSAGLKPKAEQLLALLREDPYRSP
                     PPFEKLVGDLSGACSRRINIRHRLVYQVIETEHTVKVLRMWSHYE"
     gene            complement(352..594)
                     /locus_tag="PK849_12370"
     CDS             complement(352..594)
                     /locus_tag="PK849_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPE66967.1"
                     /translation="MSDITATEARKRLFQLLDEVADTHVPVRIAGKRNAAVLVGESDW
                     RAMEETLFLCSIPGMRDSIIEGMATPVEECAEDIGW"
     gene            765..1349
                     /locus_tag="PK849_12375"
     CDS             765..1349
                     /locus_tag="PK849_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPE66968.1"
                     /translation="MRNGIWGAALCAILSAAIFLAGVFAVKLYAQEIPGTSVGRKAAI
                     GIMSREISAAMTPWKIKGPIRKATEYSRHIWDACELYKVDPFLVAALVCVESEGRADA
                     VSHSRVGLMQVNVQENMAWITKHFPKIKTVDDLLKARNNVMAGAFALSAAMRSSEDVR
                     TALFKYLGTEDERQVGKILDVVGRMNTAAQAGRK"
     gene            1470..2369
                     /locus_tag="PK849_12380"
     CDS             1470..2369
                     /locus_tag="PK849_12380"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HPE66969.1"
                     /translation="MLSVSDVVFSFSPEHPPVARVSPGDMVRFMTKDCFCDQVTGASD
                     LVTAIDFDRVNPATGPVFVEGAEPGDTLCAEILSIDVAEKGIACTLPDSGPLIGWCEL
                     RTKVIEVREGKAIFNDLVFPVRPMVGVIGTAPAEGAVVCGQPGAHGGNLDCKFMTAGA
                     KAYFPVRTRGALFQLGDLHASMGDGEIGGTGIEIGGTVLVRLHLIKNLPLDWPMLETG
                     DMIHTLASGPEFVAVQREAARQMQRLIAAAWGWDETDAALYLSVRGDSAICQTCQPST
                     FDMVLRMGIPKEPGKARLLPLPG"
BASE COUNT          472 a          683 c          775 g          490 t
ORIGIN      
        1 gagaagagcg aaaagcgagg agcacaaagg attaggggtt ccaatctgcg gacttccgag
       61 gagcccggta ctctcccgta gttgtacgct attcgtaatg gctccacatg cggagcactt
      121 ttaccgtatg ctccgtttcg atcacttggt agaccagacg atggcggatg ttgattcggc
      181 gggaacatgc gcctgaaaga tcgccgacga gcttttcaaa aggcggagga gatcggtagg
      241 ggtcttcacg cagtagcgcg aggagctgtt ccgccttggg tttcagcccc gccgaaagca
      301 gcttttctgc atctttcaga gccgccttgg tgaaaacgac tctccacgat cctaccatcc
      361 gatatcctcg gcgcactctt ccaccggtgt tgccattcct tcgatgatgg agtcgcgcat
      421 tccagggatg ctgcacagaa agagcgtctc ttccatggcc ctccagtcgg actcgccgac
      481 cagcacggcc gcgttgcgtt tcccggcgat gcgcaccgga acgtgggtat ccgccacctc
      541 gtcaagaagc tgaaagagcc gttttcgggc ttccgtcgct gtgatgtcgc tcatatcctc
      601 acctcctgtg gtacgttcta tggtacatga tgtctgcggc gaggtcaaac gccggagtgc
      661 agcgtcacca ccgttcgctc ttcccaagaa gccgtatttg tcacgatgcc ttcctctttc
      721 ttcgatgcta tcatgctcga aaacatgcga aggagcgtgt gctcatgcga aacggaatat
      781 ggggcgctgc tttgtgcgcg atcctgtcgg cggcgatttt tctcgcggga gtgtttgctg
      841 taaaactcta tgctcaagaa atcccgggaa cgtcggtcgg aagaaaagcc gccatcggca
      901 tcatgtcgcg cgagatcagc gcggcgatga ccccgtggaa gatcaagggg ccaatacgga
      961 aggcgacgga gtactcgcgg catatttggg acgcctgcga actctacaag gtcgacccct
     1021 ttctggtcgc cgcgctggtc tgcgtggagt cggaggggcg ggcggacgcc gtttcgcatt
     1081 cacgggtcgg cctgatgcag gtgaacgtgc aggagaatat ggcctggatc acgaagcact
     1141 tcccgaagat caagaccgtg gacgacctgc tcaaggcgcg gaacaacgtg atggcggggg
     1201 cgttcgccct ctccgccgcg atgcggagtt cggaggacgt ccggacggcg ctgttcaaat
     1261 acctcggtac ggaggatgaa cggcaggttg ggaagatact cgacgtcgtc ggtcgaatga
     1321 acacggcggc gcaggccgga agaaaataag agaaagaatg cggaaatcgc ggtgttttga
     1381 agtacggggg gaccccgtcc acgtgttcct ttccttgagc gctcctccct gctacaatgt
     1441 gaagaaacac ctcagaagga ggttccgcca tgctttcagt ttccgacgtg gtcttttcct
     1501 tttcgcccga acatccgccc gtagcgcggg tgagtcccgg ggatatggtg cgcttcatga
     1561 cgaaggactg tttctgcgat caggtgacgg gagcgtccga cctcgtgacg gcgattgatt
     1621 tcgacagggt gaaccccgcc acaggcccgg tcttcgtgga gggcgccgag ccgggggata
     1681 cgctgtgtgc ggagattctt tccatcgacg tcgcggaaaa gggaatcgcc tgcacgctgc
     1741 cggattccgg gccgctgatc gggtggtgcg agctgcgcac caaggtgatc gaggtacgcg
     1801 aggggaaagc gatcttcaac gacctcgtct tcccggtgcg tccgatggtc ggcgtcatcg
     1861 gaacggctcc cgccgagggc gcggtggtct gcggccagcc cggagcgcac ggaggcaacc
     1921 tcgactgcaa gttcatgacc gcgggcgcca aggcgtattt ccccgttcgg acacggggcg
     1981 cgctctttca gctcggcgat ctgcacgctt cgatgggcga cggcgagatc ggcggtacgg
     2041 ggatcgaaat cgggggaacg gtgcttgtcc ggctgcactt gatcaaaaat ctgccgctcg
     2101 actggcctat gttggagaca ggcgacatga tccatacgct cgccagcggt ccggagttcg
     2161 tcgctgtaca gcgggaggcc gcacgccaga tgcagcggct gatcgcggcg gcctgggggt
     2221 gggacgaaac ggacgcggcg ctttaccttt ccgtgagggg cgattccgcg atctgccaga
     2281 cctgccagcc cagcacattc gacatggtgc tgcgcatggg aataccgaaa gaaccgggga
     2341 aggcgcgcct tctccccctg ccgggataaa gtgaaaagag aggcttcccg tccgggaggc
     2401 ctctctcgaa tttccgtatg
//
LOCUS       DAOPKU010000470         2419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_50517_length_2419_cov_4.753807, whole genome shotgun sequence.
ACCESSION   DAOPKU010000470 DAOPKU010000000
VERSION     DAOPKU010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50517_length_2419_cov_4.753807"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..402
                     /locus_tag="PK849_12385"
     CDS             7..402
                     /locus_tag="PK849_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPE66970.1"
                     /translation="MTTAIERTLTDFFEEEQSVRLAFLMGSFAKETARPDSDVDVAAL
                     FDRTPDFMETLDLRERLSALLRRETDLVVLNDAGPIIGMQALKTGIRLFARKNEYEEF
                     FVRTLNEYDDLKRLRAPIEEAVLRRRISA"
     gene            413..592
                     /locus_tag="PK849_12390"
     CDS             413..592
                     /locus_tag="PK849_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66971.1"
                     /translation="MLAKVATIQRCLARIRDVTSLDPARLYDGYRAMAMDAEREKEAA
                     EWCDALSRGAIDEAR"
     gene            complement(610..2073)
                     /locus_tag="PK849_12395"
     CDS             complement(610..2073)
                     /locus_tag="PK849_12395"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66972.1"
                     /translation="MLAFTDEKLLNLLIQYNPWWRGVPISEKTSPPKRRIAYYEAEKM
                     LLHPSLRRFVILSGARRVGKTTIMYQLIQSLLQRGVPAKSIVYLSFDNPIIKLGGFES
                     VLRAYRNAYPTEERCFFFFDEVQYAEDWEAWIKTLYDTQPELRLVATGSASPALEKGS
                     AESGVGRWSVLKIPTLSFYEYCDLLQLPERPRLEQGIPPTRLHTMDKGDLSELMAGLS
                     PLQRHFNRYLTVGGFPELALSQDDAFSQRMLREDVVDKVIKRDILALFNVRNPLQLEK
                     VFLYLCMHSSGIVNMTTMSKELDGMNKSTLTNYIQFLKEANLIYISDPVGVDGKGVLK
                     GRPKIYVADAAIRSAVLMLENAIADAGETGIMVETTVYKHIAAFYSAANARVGYYRRV
                     KDGEKEVDVVVELPAGRILCEVKYRENTHVKGTDAIIELADDERANVLGALIITKRAE
                     DYGVAAHATRTPVVRIPAPAFLYLLGNAEKEGDAAKM"
BASE COUNT          514 a          703 c          660 g          542 t
ORIGIN      
        1 tacgacatga cgacggccat cgagcgaacg ctcacggact tcttcgagga ggagcaatcc
       61 gtccgcctcg ctttcctgat gggttccttc gccaaggaga cggcccgtcc ggacagcgat
      121 gtggacgtcg ccgcgctttt cgaccggact ccggacttca tggagacact cgatcttcgg
      181 gagcggcttt cggcgttgct ccgccgcgag accgatctgg tggtgctgaa cgacgcgggg
      241 ccgatcatcg ggatgcaggc gctgaagacc gggatcaggc tcttcgcccg gaaaaacgag
      301 tacgaggagt tcttcgtccg cacccttaac gagtacgacg acctgaagcg cctccgcgct
      361 ccgatcgaag aagccgtgct gaggaggaga atctctgcct gacaaagacg tggtgttggc
      421 aaaagtcgcg accatccaaa ggtgtctggc gcggatacgc gacgtcacgt cgctcgatcc
      481 ggcacgtctc tacgacgggt acagggccat ggcaatggat gcggagcgcg aaaaagaggc
      541 tgcggaatgg tgcgacgctc tttcgagggg cgcgatcgat gaagcacggt aagcgctgaa
      601 agcgagactc tacatcttcg cggcatcccc ttctttttcg gcgtttccga ggaggtagag
      661 aaatgcgggg gcgggtatgc gcacgacggg cgtccgcgtt gcatgggcgg cgactccgta
      721 atcctcggcc cgtttcgtga tgatcagcgc tcccagtaca ttcgcgcgct cgtcgtccgc
      781 gagttcgatg atcgcgtccg tccctttgac atgcgtattc tcgcggtatt tcacttcgca
      841 cagtatccga cccgccggaa gctccaccac cacgtccacc tctttttctc cgtccttgac
      901 cctcctgtag tagccaacgc gcgcgttcgc cgcggaatag aacgcggcga tgtgcttata
      961 gaccgttgtt tccaccatga tccccgtctc cccggcgtcg gcgatcgcgt tctccagcat
     1021 cagcacagcg cttcgtatag cggcatcggc cacatagatc ttcggtcgcc ccttcagtac
     1081 acccttcccg tcgacaccta cgggatcgct gatatagatc aggttcgcct ctttcaggaa
     1141 ctgaatatag ttcgtcaacg tgctcttgtt cattccgtcc agctccttgg acatcgtcgt
     1201 catattcacg atgcccgaag agtgcatgca gaggtacaag aagacttttt cgagctggag
     1261 agggttccgt acattgaaaa gagccagaat atcccgtttg atcaccttgt cgacaacatc
     1321 ctcgcgcagc atcctctgag aaaaggcgtc atcctgcgac agcgcaagtt ccggaaagcc
     1381 gccgaccgtc aggtacctat taaagtgacg ctgaagcggc gaaagtccgg ccatcaattc
     1441 gcttaaatcc cccttgtcca tcgtgtgcag cctcgtcggt ggaataccct gttcaaggcg
     1501 gggcctctcg gggagttgca gcagatcgca gtactcgtaa aaggaaagcg tcgggatctt
     1561 caagacgctc caacgtccta cgccgctctc cgccgatcct ttttccagag cggggctcgc
     1621 cgaccccgtg gcgaccagcc gcagctccgg ttgcgtgtca tacagcgttt tgatccacgc
     1681 ctcccagtcc tccgcgtact gcacctcgtc gaagaagaag aaacaccttt cctcggtcgg
     1741 ataggcgttc cgatacgccc gcaacacgct ctcgaaaccg ccaagtttaa tgatgggatt
     1801 gtcgaacgac agatacacga tgcttttcgc gggcacgcct ctctgtaaga gactttgtat
     1861 cagctggtac atgatcgtgg tcttgcccac gcgtctggcg ccggagagta tcacgaaccg
     1921 gcgcagagag gggtgaagca gcatcttctc cgcttcgtag taggcaatcc gccgtttggg
     1981 cggagatgtc ttctctgaaa tgggaactcc tctccaccag ggattgtact gtattaaaag
     2041 attcaacagc ttttcatccg tgaacgcaag catcgaggat cacctcccgg aaaatagtat
     2101 atcgtactct cgatttagtc aataataatt cacatcgtac tgttttttaa agtggatgcg
     2161 ggagggtgca ctttctgttt tctctacgat agaagaaacc gtcgaggttc acagagtgat
     2221 tcacggacgc cgccgctacg cccatctttt ggggaacgga gcgtagagtc agactgccgc
     2281 gaacgctttg tgctccgctt cagaagcgtt ctcaagagag aaatccgtct ttttgaaacg
     2341 actccgctct ttttacaacg ttcacgataa ggcagtacaa cacggagtga atgtatggta
     2401 tcattctcac gaagatgcg
//
LOCUS       DAOPKU010000471         2415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_50629_length_2415_cov_4.004237, whole genome shotgun sequence.
ACCESSION   DAOPKU010000471 DAOPKU010000000
VERSION     DAOPKU010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50629_length_2415_cov_4.004237"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..941
                     /locus_tag="PK849_12400"
     CDS             141..941
                     /locus_tag="PK849_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPE66973.1"
                     /translation="MEKKERSEGWKMQSVVKAFGLLKLFTGKNPEWTFNQLLQEKRCP
                     KSTLSNMLRTLEFCGVLEKEKTSQLYRLGLDVLEMSYSVRKALPIIEYAMKFLDDVQQ
                     KTGGIVYFTIPKDGRVLYLDASYPANKNVTYSITGKTLDMHCTGVGKAMLSKMSDEQI
                     RRIIAEHGMKQYTPRTITDFDELMEEIGRIRARGYALDFGEESQGVKCVAVPVVSGRM
                     LLGAISISGSIISLKEEQYEEYAEMLFEIANILADKADMFPRAYLCGV"
     gene            complement(955..2031)
                     /gene="corA"
                     /locus_tag="PK849_12405"
     CDS             complement(955..2031)
                     /gene="corA"
                     /locus_tag="PK849_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPE66974.1"
                     /translation="MSGGGKRGTGRRAKLPGLPTEKQLVAEERKQTTSGIEIMHYAPD
                     GFEEIRNATLEDCARLKDCPGITWINVDDIRDTSTVRSLAALFGLHPLTTEDIGNPAQ
                     RPKAENFEQYLFFALKMLTYDDATHSILNENVSVVLGDRWVVSFQEREGDVLDPVRDR
                     IRSGKGRLRSEGADYLAYAIMDSVVDEYFVVLEKLGDHIEEIDEQILAVPDRTHMKEL
                     HRLKREILFLRKAVWPLREEISTIEKSGSRLVGDSIKPFLRDLYDHTIEIIDMVETNR
                     DIVSGMHDTFLSAVSNRMNEIMKVLTIIGTIFIPLTFIAGVYGMNFEHMPELKWPRGY
                     YGVMAFMAALALSMLAAFKKRKWL"
     gene            complement(2028..2393)
                     /locus_tag="PK849_12410"
     CDS             complement(2028..2393)
                     /locus_tag="PK849_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPE66975.1"
                     /translation="MTLLLKRVYEAQSPEDGFRILIDRLWPRGITKEKAAVDLWLKEA
                     APSPELRKAFCHDPEKWDDFRARYVAELETNAAVDTIMERLREGTVTLVYAAKDETYN
                     HAVVLAEHLERQIAVRNGA"
BASE COUNT          450 a          719 c          743 g          503 t
ORIGIN      
        1 gtgatgtcgc agggcagcta cagcatcttc ttcacgcgcc ccgtcacggt gacgctgctc
       61 gctctgacgg cgctctccat actgttcccc ctgatcagaa atcatttttc cgcggcgaag
      121 ataaaggaac gtgattgacg gtggaaaaaa aggagcgatc cgagggctgg aagatgcagt
      181 ccgtagtcaa ggcgttcggg ctgctgaagc tgtttacggg gaaaaatccg gagtggacgt
      241 tcaaccagct gcttcaggag aaacggtgtc cgaagagcac gctctcgaat atgctgcgga
      301 ctttggagtt ctgcggcgtt ctcgagaagg agaagacgag ccagctctac cgactggggc
      361 ttgacgtgct cgaaatgagc tacagcgtcc gcaaggccct cccgatcatc gagtacgcga
      421 tgaagttttt ggacgacgtg cagcagaaga ccggcgggat cgtctacttc accattccca
      481 aggacggcag ggtgctctac cttgacgcga gctatccggc gaacaagaac gtgacctact
      541 cgatcaccgg gaagacgctg gacatgcact gcaccggggt gggcaaggcg atgctgagca
      601 agatgtcgga cgagcagatc cggcggatta tcgccgagca cggtatgaaa cagtacaccc
      661 cgaggacgat cacggacttc gacgagctga tggaggagat cgggagaatc cgcgcgcggg
      721 gctacgccct ggacttcggg gaggagtcgc agggtgtgaa gtgcgtcgcc gtccccgtcg
      781 tttcaggacg gatgcttctg ggagcgatca gcatctcggg gtcgatcatc agtctcaagg
      841 aagagcagta cgaggagtac gccgagatgc tgttcgaaat cgccaacata ctggcggaca
      901 aggcggatat gttcccgagg gcgtacctct gcggcgtgtg aggccgacgc tcggctacag
      961 ccacttccgc ttcttgaagg ccgcgagcat gctcagggcg agcgccgcca tgaacgccat
     1021 gaccccgtag tacccccggg gccacttcag ctcaggcatg tgttcgaagt tcatcccgta
     1081 gacgcccgcg atgaaggtca gcgggatgaa gatcgtcccg atgatggtga gcactttcat
     1141 gatctcgttc atccggttgc tgaccgccga gaggaacgtg tcgtgcatgc cgctcacgat
     1201 gtcgcggttg gtctccacca tgtcgatgat ctcgatggta tggtcgtaca ggtcgcgaag
     1261 gaagggtttg atcgagtcgc cgacgaggcg cgagccgctc ttctcgatag tcgaaatctc
     1321 ctcccgcagc ggccagactg ccttccgcag gaagagaatc tcacgcttca ggcgatggag
     1381 ctccttcatg tgcgtcctgt ccggaacggc gaggatctgt tcgtcgatct cctcgatgtg
     1441 gtcgccgagc ttttcaagga cgacgaaata ctcgtccacc accgaatcca tgatggcgta
     1501 ggccaggtag tcggcgcctt cgctccggag gcgtcccttc cccgaccgga tgcgatccct
     1561 gacgggatcg agcacgtccc cctcgcgctc ctggaatgag acgacccatc ggtcgcccag
     1621 cacgacgctg acgttctcgt tgaggatcga gtgcgtcgcg tcgtcgtagg tcagcatctt
     1681 gagggcgaag aagagatact gctcgaagtt ctccgccttg ggacgctgcg ccgggttgcc
     1741 gatatcctcc gtcgtcagcg ggtgcagtcc gaaaagggcc gccaggctcc ggactgtact
     1801 cgtgtcgcga atgtcgtcga cgttgatcca tgttattcct gggcagtctt tcagccgtgc
     1861 gcagtcttcc agcgtcgcgt tgcggatctc ctcgaaaccg tccggagcgt agtgcatgat
     1921 ctcgatgccg gaagtcgttt gtttccgctc ttcggcgacg agctgtttct ctgttggaag
     1981 gccgggcagc ttcgctcggc gtccggttcc gcgctttccg cctccgctca tgcgccgttc
     2041 ctcactgcga tttgccgctc cagatgctcc gcaagcacga cggcgtggtt gtatgtctcg
     2101 tccttcgcgg cgtagacgag cgtgaccgtc ccctcgcgca ggcgctccat gatcgtatcg
     2161 accgccgcat tcgtctccag ctcggcgacg tagcgcgccc ggaaatcgtc ccacttttcg
     2221 gggtcgtggc agaacgcttt ccgcagttcc gggctgggcg cggcctcctt cagccacaga
     2281 tcgacggcgg ccttctcctt tgtgattccg cgcggccaca gccggtcgat aagaatccgg
     2341 aagccgtctt ccggactttg tgcttcgtac acccgtttca gcagcagcgt catgctcttc
     2401 cctccccgca ggtct
//
LOCUS       DAOPKU010000472         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_50931_length_2405_cov_4.387660, whole genome shotgun sequence.
ACCESSION   DAOPKU010000472 DAOPKU010000000
VERSION     DAOPKU010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50931_length_2405_cov_4.387660"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PK849_12415"
     CDS             complement(<1..905)
                     /locus_tag="PK849_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE66976.1"
                     /translation="MTQPTTYRTPAFFRRIPMLLAASLFLAALLFSSPLCGESLTVKV
                     ALHSGKPGIFIDESGTPAGIFVDILEHIAGIEGWKLEYVPGTWAEGLERLKQGEVDIV
                     TSIAFTPEREKIYTFTGTPALSAWSAAYVARGSGIVSLLDLKGKRVAVMKNTVQESAF
                     IKHIQGFDLDIEILSVPDQDAAFSMVADGTADAAIEVSTTGEVYRQRYELENSAIVFN
                     AVQAYYAAAREDPKGLASAINRHLYAMKADPSSPYYEIMKRWFPKKTGRSAVERRLWI
                     ASGVFLAGLLLSLSMLAALRWQVQRR"
     gene            complement(948..>2405)
                     /locus_tag="PK849_12420"
     CDS             complement(948..>2405)
                     /locus_tag="PK849_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE66977.1"
                     /translation="AKEAAEQANRTKSAFLANMSHEIRTPLNAVLGFASILERDGGLS
                     ETQTDQVRTINRSGRHLLKLINDILDMSRIESGQLELSVTEFCLRDLLDDMETMFRSR
                     TDAKGLHFVMERMESVPRYAAGDEAKLRQILFNLLGNAVKFTKTGGIAVRARADKENE
                     AGDALRLLVEIEDTGPGIPEKDLEHIFDAFRQSEAGRDAGGTGLGLSISKRLAELMGG
                     SLTVQSKIGTGTCFTLSVPLHTATSGHRTAKMDRQDIVGLDTGDAFFRILIVDDVKEN
                     RDVLRAMLSPDRNAGSATMFRGRTSAFELREAVNGEEALSIFEEWFPHVVLMDMRMPV
                     MDGYEATRRIKSVPGRGSVPVIAITASAFEDDRDAVLKAGVDRYIRKPLRAEELFETL
                     GDVLGLPVLRGGDSAKASGSKGTLPTEEEMKALPGELLAAMHEAVENGDIAGLDELIV
                     KTREIDAGIAEKLRTLARRYDYETLSRLLKNGERG"
BASE COUNT          402 a          757 c          732 g          514 t
ORIGIN      
        1 cgtcgctgta cctgccagcg gagcgccgcc agcattgaca gactgagaag caaccccgcc
       61 agaaaaacgc ccgaggcgat ccagaggcgg cgttccaccg cgcttctgcc ggtctttttc
      121 gggaaccagc gcttcatgat ctcgtagtag ggcgaggagg gatccgcttt catcgcgtac
      181 agatgccggt tgatggccga tgcaaggcct ttcgggtctt cccgggcggc ggcgtaatag
      241 gcctgtaccg cgttgaagac gatggcgctg ttctccagtt cgtagcgctg tctgtagact
      301 tcgcccgtcg tgctgacctc tattgcggcg tcggcggttc cgtcggcgac catggagaac
      361 gccgcgtcct gatccggaac ggagaggatc tcgatatcga ggtcgaagcc ctggatgtgc
      421 ttgatgaagg ccgactcctg cacagtgttc ttcatcaccg cgacgcgctt ccccttcagg
      481 tcgagcaggg agacgatgcc gctccctctg gcgacgtacg ccgcggacca tgcggagagc
      541 gccggagtcc ctgtgaacgt gtagatcttc tcccgttcgg gggtgaatgc gatgctcgtg
      601 acgatatcga cttccccctg cttcaggcgc tccagcccct ccgcccatgt gccggggacg
      661 tactccagct tccagccttc gattccggcg atatgttcga ggatgtcgac gaagatgccg
      721 gcaggggttc cggattcgtc gatgaagatg ccgggtttgc ccgaatgcag ggcgactttg
      781 accgtaaggc tctctccgca aaggggggaa gaaaagagaa gcgctgccaa aaacagcgag
      841 gcggcaagca gcatcggtat gcggcggaag aacgccggag ttcggtatgt cgtcggctgg
      901 gtcatcgttt tcatctcctt cattttcggg aggccggcat gggatgctca gcctctctcc
      961 ccgttcttca gcaggcggga gagcgtttca taatcatacc gcctggcgag cgtcctgagt
     1021 ttttcggcga tcccggcgtc gatctccctc gtttttacga taagctcgtc gagacccgcg
     1081 atgtctccgt tctcgaccgc ctcgtgcatc gcggcaagca gttctccggg aagggccttc
     1141 atctcctctt cggtcggaag cgttcccttg cttccggacg cctttgcgga atcgcccccg
     1201 cggagaaccg gcaggccaag aacgtctccc agcgtctcga acagctcctc cgccctgagc
     1261 ggttttcgta tgtatctgtc caccccggcc ttcagcacag cgtccctgtc gtcttcaaag
     1321 gcgctcgccg tgatcgctat gacggggacc gagccccttc ccggaaccga cttgatccgc
     1381 cgcgtggctt cgtagccgtc catgaccggc atgcgcatat ccatcagcac gacgtgaggg
     1441 aaccactctt cgaaaatcga cagcgcctct tcaccgttga cagcttcccg cagttcgaag
     1501 gcggaagtcc tcccccggaa catcgtcgcg gaaccggcgt tccggtcggg agagagcatc
     1561 gcgcgcagca cgtcgcggtt ctccttgacg tcgtcgacga tcagtattct gaagaaggcg
     1621 tctcccgtgt ccaggccgac gatgtcctgc cggtccattt tcgctgtacg atgtcccgac
     1681 gttgccgtgt gcagaggaac ggagagggtg aaacacgttc ccgtcccgat tttgctttgt
     1741 acggtgaggg agccgcccat cagctcggcg agccgcttgc tgatcgaaag gccgagtccc
     1801 gtccctcccg cgtcgcgtcc ggcctcggac tggcggaatg cgtcgaagat gtgctccaaa
     1861 tccttctccg gaatgcccgg tcccgtatcc tctatctcca cgaggagacg caaggcgtcc
     1921 cccgcttcat tctccttgtc tgcacgggcg cgtacggcga tgccgccggt tttggtgaac
     1981 ttgacggcgt tcccgagcag gttgaagagt atctgccgca gtttcgcctc gtcccccgcc
     2041 gcgtagcggg gaacgctctc catgcgctcc atcacgaagt gcagtccctt ggcgtcggtc
     2101 cgggagcgga acatcgtctc catgtcgtcc agcaggtccc ggaggcagaa ctccgtcacc
     2161 gagagctcaa gctgtcccga ctctattctg gacatgtcga gaatgtcgtt gatcagtttc
     2221 agcaggtgcc gcccgctccg gttgatggtg cgcacctgat cggtctgcgt ttcggagagg
     2281 ccgccgtcgc gttcgagaat ggaggcgaac ccgaggaccg cgttgagcgg cgtgcggatc
     2341 tcgtggctca tgttggcgag gaaggcgctc ttggtacggt tggcctgttc cgccgcctct
     2401 tttgc
//
LOCUS       DAOPKU010000473         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_51062_length_2401_cov_3.544757, whole genome shotgun sequence.
ACCESSION   DAOPKU010000473 DAOPKU010000000
VERSION     DAOPKU010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51062_length_2401_cov_3.544757"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..770)
                     /locus_tag="PK849_12425"
     CDS             complement(162..770)
                     /locus_tag="PK849_12425"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPE66978.1"
                     /translation="MGEWWSGMLMFEKVFWYIAIPFTALFFIQLVLTFTGMGGEDADG
                     GMEGDAGGEDFDVEGDGDGDIGEAGGFQIFTVKNFITFFTVFGWSGITLHNAGANEYL
                     VLLGSLLIAVAVMLLVALLFVSITRLAQSGTMDVKNALGRTGEVYLPIPAGRAGRGKI
                     SITLQGALREVDAVTAGEALPRGTSVRVVDVAGNGVLLVEKA"
     gene            complement(813..2246)
                     /locus_tag="PK849_12430"
     CDS             complement(813..2246)
                     /locus_tag="PK849_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPE66979.1"
                     /translation="MKSIQILCTFLFLLVFLSALPALGAEVVSSTEADQTGVEVTVYN
                     TDLALVKDVRSILLKEGQGELRFMDVASSIRPETVHVKSLNNPPSLAILEQNYEYDLI
                     SPEKLLEKFVGRTVKIEVWNDYQGKKEIVEGTLLSTNNGLVFDIGGQIYLNYPGLKIV
                     PSIPDNLYARPTLAWLYANSAKEKHSVEVSYLTGGMTWKADYIFVLDDADTAADLTGW
                     VTLNNRSGAAYRDATLKLIAGEVQTVADVLPPPMMRMAMGKGVPEAAPAFEEEAFFEY
                     HIYNLARKTTIGNNQTKQISLLEAFGTKIGKEYITRGERRYFTSSLQRGLLKQKVDVV
                     ISFKNSEENNLGMPLPKGIIRMYKKDSKGSLQFIGEDRIDHTPRNEEVRLKVGEAFDV
                     VAERRQTRYKKLADNLYETSWELIVRNRKKDESVVVSFNEPVWGDWEVIASTHPYEQT
                     DAFTLRFDVPAKPDEEVVVKFSLRIRS"
BASE COUNT          433 a          742 c          708 g          518 t
ORIGIN      
        1 ccttggcgac ctttccgtag accacgagga tcttgtcgga ggggcacttc ttgtagcgcg
       61 cgaagagcgc cgccacggtg gtgaagatga tgatgactat cagggtgatc agaatcagac
      121 cgaacgtcat gcgaatcctc tcctttcaga tgttgggtgt attacgcttt ctcgaccagc
      181 agcacgccgt tgccggcgac atccacgacc cgcaccgagg ttcccctggg gagcgcctcc
      241 cccgccgtca cggcgtcgac ctcgcggagc gcgccctgca gcgtgatgga gatttttcca
      301 cggccggcac gtccggcggg gatgggaagg tagacttcgc ccgttctgcc gagcgcgttc
      361 ttgacgtcca tcgtcccgct ctgcgccagc cgcgtgatgg agacgaagag cagcgcgacg
      421 agcagcatga ccgcgacggc gatgagcagc gatcccagga ggacgagata ttcgttcgct
      481 ccggcgttgt ggagcgtgat gccgctccag ccgaagacgg tgaagaaggt gatgaagttc
      541 ttcacagtga agatctggaa acctccggcc tcgccgatat ccccgtcgcc gtcaccctcg
      601 acgtcgaagt cctctcctcc tgcgtcgcct tccattccgc cgtccgcgtc ctccccaccc
      661 atgcccgtga aggtgagcac gagctggatg aagaagagcg cggtgaaggg aattgcgata
      721 taccagaaga ctttttcaaa catgagcatg ccgctccacc actcccccac tcacaccgcc
      781 tcctttgcgc cgacagggtt tcgcatgttt cgtcagctgc ggattcgcag gctgaacttg
      841 accacgacct cttcgtcggg tttggcgggg acgtcgaagc ggagcgtgaa ggcgtcggtc
      901 tgttcgtacg gatgggtcga tgcgatcact tcccagtcgc cccagacggg ctcgttgaag
      961 gagacgacga cagactcatc ctttttgcgg tttctgacga taagctccca cgacgtctcg
     1021 tagaggttgt cggccagctt cttgtagcgc gtctgacggc gttccgcgac cacgtcgaag
     1081 gcctcgccca ccttcagacg gacctcctcg ttgcgcggcg tgtggtcgat gcggtcttcg
     1141 ccgatgaact gaaggcttcc tttggagtct ttcttgtaca tcctgatgat ccccttggga
     1201 aggggcatgc cgaggttgtt ctcctcggaa tttttgaagg agatgacgac gtccaccttc
     1261 tgcttcagca acccccgctg gagcgaggag gtgaagtagc gccgctcgcc gcgggtgatg
     1321 tactccttgc cgattttcgt gccgaacgcc tccagcaggc tgatctgctt ggtctggttg
     1381 ttccctatcg tcgtctttcg cgccagattg tagatgtggt actcgaaaaa agcctcttct
     1441 tcgaaggcag gcgcggcttc gggtaccccc ttccccatcg ccatccgcat catgggcgga
     1501 ggaagaacgt cggcgaccgt ctgcacttcg ccggcgatca gcttgagcgt cgcgtcccgg
     1561 tacgccgcgc cgcttctgtt gttcagcgtg acccagccgg tgaggtcggc tgccgtgtcg
     1621 gcgtcgtcga ggacgaagat gtagtccgcc ttccacgtca tcccgcccgt caggtaggag
     1681 acctcgacgc tgtgcttctc cttcgccgag ttggcgtaga gccacgccaa ggtggggcgc
     1741 gcatagaggt tgtcaggaat ggagggaaca atcttcagtc cggggtagtt caggtagatc
     1801 tggccgccga tgtcgaagac gagtccgtta ttcgtgctca gaagcgtgcc ctccacgatc
     1861 tccttcttcc cctggtagtc gttccacacc tcgatcttca ccgtgcgccc gacgaacttc
     1921 tccagcagct tctccgggct gatgaggtcg tactcgtagt tctgctccag aatcgcaagc
     1981 gaaggcgggt tgttgagcga cttcacatgc accgtctccg gccggatgga agaagccacg
     2041 tccatgaagc ggagctcccc ctgcccctct ttgagcagga tgctcctgac gtccttcacc
     2101 agggcgagat ccgtgttgta caccgtgacc tcaacccccg tctgatctgc ttccgtgctc
     2161 gagaccacct cagcccccag tgcaggcagg gcggagagaa agacgagcag aaaaaggaac
     2221 gtgcacagta tctgaatact tttcatttca cagacctcct tttttagttt gtcgcaattt
     2281 ttaggaacac acgctctgtt catcgctctc cggctgcgtc ccgcccgcgg attccgcgag
     2341 ggcgtcgagc agaaggagca ggcgcatcag gtagagcagc acgccgccgt tcgcggcgag
     2401 a
//
LOCUS       DAOPKU010000474         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_51311_length_2393_cov_4.141574, whole genome shotgun sequence.
ACCESSION   DAOPKU010000474 DAOPKU010000000
VERSION     DAOPKU010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51311_length_2393_cov_4.141574"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK849_12435"
     CDS             <1..279
                     /locus_tag="PK849_12435"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPE66980.1"
                     /translation="CGALLGGACLLGFYAGKGNDGETEHPMFRMMVRRLTEWFRDDLC
                     ASGSVLCGDILNEHGRARCPQLVRSVWGKAMELLLENDIDPTEGRPEE"
     gene            276..533
                     /locus_tag="PK849_12440"
     CDS             276..533
                     /locus_tag="PK849_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66981.1"
                     /translation="MSFPKRTRSLCPVCMKPVDAVYQPEGRDIFLEKQCPEHGRFRTI
                     VWRGPLSLDEWSGGEIPEHPFTPSSRCPLDCGACEAHEAFG"
     gene            540..1559
                     /locus_tag="PK849_12445"
     CDS             540..1559
                     /locus_tag="PK849_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE66982.1"
                     /translation="MVEVTRRCDLRCPVCFASAGEGEDPPFEHMAGIFKKIAARAPGS
                     IVQISGGEPAMRDDLPELVAEAAGMNFAGVQLNTNGLRLASEPGFARTLKEHGLSWVY
                     LQFDGTDDAVYERLRGRPLFDLKRRAVERCAEAQLGVVLVATVRRGTNDHLLGDIIRF
                     GTAHAPTVRGVHFQPMAFFGRYPEAPKDGDRTTLADVLRSVCEQHGFFRMEDFSPCGG
                     LHPLCTFTGEFLVSGGELARKETERVHEELDLVRATSRVASRWTLPSPSGGGAEDGFE
                     KILSWRRRIFTITAMAFQDAWTIDTERLQRCPIKYVAHDGRFFPFCAYNATSTDGRSL
                     YRKDA"
     gene            1556..>2393
                     /locus_tag="PK849_12450"
     CDS             1556..>2393
                     /locus_tag="PK849_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66983.1"
                     /translation="MRPFLDEWIAGMTEEPFCRERLERYQLERLRQTVELVRAESPFY
                     RERLASLPDGFPSSLDDFTRLPPTLPGDLAAAPEQFHAAPQDKIARIVTLRTSGSTGE
                     GKRLFFTGDDLDETVDIFRIGMATFVRPGGRALVLLPGERPGSIGDLLVRALAPEREC
                     ILCPASDERSVLERIAESKAECLVGLPTQVLSLARCLHSPLGRTLSQVLLCADYAAPS
                     LVKAVENAWECVVRRHYGLTETVYGGALECEERDGLHVMEGNYLFEILNPETLTPTLP
                     GEF"
BASE COUNT          417 a          822 c          753 g          401 t
ORIGIN      
        1 tgcggcgcgc tgctcggcgg cgcatgcctc cttggattct acgccgggaa ggggaacgac
       61 ggcgaaacgg agcacccgat gttccggatg atggtccgcc gcctgacgga gtggttccgg
      121 gacgacctgt gcgcctccgg aagcgtcctc tgcggcgaca ttctcaacga acacggacgc
      181 gcccgctgtc cgcagctcgt ccgctccgtc tggggcaagg cgatggagct gcttttggag
      241 aacgatatcg acccgaccga aggacggccg gaggaatgag cttcccgaaa cgcacgcgca
      301 gcctctgccc cgtctgcatg aagcccgtcg acgccgtcta ccaaccggag gggcgcgata
      361 ttttcctgga aaaacagtgc cctgaacacg gccgcttccg gaccatcgtc tggaggggac
      421 cgctctccct ggacgagtgg agcggcggcg aaattccgga acacccgttc accccctcat
      481 cccgctgccc ccttgactgc ggcgcatgcg aggcgcacga ggccttcggc tgatctttcg
      541 tggtcgaggt cacacgtcgc tgcgaccttc gctgccccgt ctgtttcgcc tccgccgggg
      601 aaggagagga tcctccgttt gagcacatgg ccggcatctt caaaaaaatc gccgcgcgcg
      661 ctcccgggag cattgtgcag atctccggcg gggagcccgc catgagggac gacctccccg
      721 aactcgtcgc cgaggctgcc gggatgaact tcgcgggagt ccagctcaac acgaacggtc
      781 tccgtctcgc ctccgaaccc ggcttcgcac gcaccctgaa ggagcacggc ctttcgtggg
      841 tgtaccttca attcgacgga accgacgacg ccgtctacga aagactcagg ggacgccctc
      901 ttttcgacct gaaacggcgc gccgtcgaac gatgcgccga ggcgcagctc ggcgttgtcc
      961 tcgtcgccac tgtgcggcgc ggcacgaacg accacctgct cggcgacatc atccggttcg
     1021 gcacggcgca cgccccgacg gtccgcggag tccactttca gccgatggcc ttcttcggca
     1081 gataccccga ggccccgaag gacggcgacc gaacaacgct cgccgacgta ctgaggagcg
     1141 tgtgcgaaca gcacggcttc ttccgcatgg aggacttctc cccttgcggc ggcctgcacc
     1201 cgctctgcac attcaccggc gaatttctgg tctccggcgg ggaactcgcc aggaaggaga
     1261 ccgagcgcgt gcatgaagag ctcgacctgg tgcgcgccac gtcgcgcgtc gcctcaagat
     1321 ggacgcttcc gtccccctcc ggcggcggcg cggaggacgg tttcgagaaa atactctcgt
     1381 ggcggcggcg gatattcacc atcaccgcga tggcgttcca ggacgcatgg accatcgaca
     1441 ccgaacggct ccagcgctgc cctatcaagt acgtcgcgca cgacgggcgg ttcttcccct
     1501 tctgcgcgta caacgcaacc tcgacggacg ggcggtcgct ctataggaag gacgcatgag
     1561 accgtttctc gacgaatgga tcgctggaat gacggaggag cccttctgcc gggaacgcct
     1621 cgagcgctac cagctggagc gccttcgcca gacggtggag ctcgtccgcg cggagagccc
     1681 gttctacaga gagcgtctcg cctctcttcc ggacggattc ccttcgtcgc tcgacgactt
     1741 cacgcgcctt cctcccactc tgcccggcga cctcgccgcc gcaccggaac agttccacgc
     1801 cgccccccag gacaaaatcg cgaggatcgt cacgctgcgc acctcgggga gcaccggcga
     1861 ggggaagcgg cttttcttca ccggggacga cctcgacgag acggtcgata tattcagaat
     1921 cggaatggcc accttcgtcc gtccgggagg gcgggccctc gtgctgcttc ccggagagcg
     1981 ccccggaagc ataggcgacc tcctcgtccg cgcgctcgcg cccgagaggg agtgcatcct
     2041 gtgcccggcg tccgacgaac gaagcgtgct tgaacggatc gccgagtcga aggccgaatg
     2101 cctcgtcggg ctgccgacgc aggtgctctc gctcgctcgt tgcctgcatt cgccgctggg
     2161 aagaacgctc tctcaagtgc tgctgtgcgc cgactacgcc gcgccgagcc ttgtcaaagc
     2221 ggtcgaaaac gcatgggagt gcgtcgtccg ccgccactac ggcctcaccg agacggttta
     2281 cggaggggcg ttggagtgcg aagagcggga cggactgcac gttatggagg ggaactacct
     2341 cttcgagatc ctgaacccgg agacgctcac ccccacgctt cccggggaat tcg
//
LOCUS       DAOPKU010000475         2389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_51431_length_2389_cov_4.661525, whole genome shotgun sequence.
ACCESSION   DAOPKU010000475 DAOPKU010000000
VERSION     DAOPKU010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51431_length_2389_cov_4.661525"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PK849_12455"
     CDS             <1..406
                     /locus_tag="PK849_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPE66984.1"
                     /translation="FGSLVVALDLEPSRTSLMLDNLREFLAQARKNPKITLLHALAPQ
                     SAEDAPEVLNAGRQALEEVRDEISSWGYETSAEIVSGDPSKELPARIRELAPSLLVIG
                     LPVAGELGELVFGSTAEMLFAGTTCPLLVFPL"
     gene            complement(431..829)
                     /locus_tag="PK849_12460"
     CDS             complement(431..829)
                     /locus_tag="PK849_12460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPE66985.1"
                     /translation="MEERSSVAPWPRTLIRRDLAGDTPMLYLGAWGKDILLGFAALGP
                     CEKGTELTNIAVLPEYRRKGIGSQLVLGVSEVALSLGYSRIYLHVRVSHEESRTFYGG
                     FDFSPLYVVPGYYLDGEDALFMEALLPLPV"
     gene            complement(905..2191)
                     /locus_tag="PK849_12465"
     CDS             complement(905..2191)
                     /locus_tag="PK849_12465"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPE66986.1"
                     /translation="MKGRVEVIIGAQWGDEGKGRVVDALGAKVDIFARYQGGANAGHT
                     VIVEGQKYVFHLLPSGMLYPCKICIIGNGVVIDPEQLLKELKDLQDQGKDRARLVISG
                     AAHVVMPYHKVLDKAQEELRGKGRKIGTTGRGIGPAYVDKYSRCGIRVEDLLDGDVLR
                     EKLEYNLDEKNTYLSKIYGEEPLPFHEMYSIALSWGKSLAPYVGDASLIIDEALREGK
                     GVLFEGAQGTLLDIDHGTYPYVTSSSPVAAGGCVGLGVGPSMVGRVIGVVKAYCTRVG
                     EGPFPTEDCGDEGNKLREKGAEFGATTGRPRRCGWLDLVALRYAVRVNGISSIALTKL
                     DVLTGQERIPVCTSYMVEGQDEPHFPCALQRLEKASPHYEYLEGWHEDIASCTSYEQL
                     PPQARKYVEHIESATGVPVNLIGVGPGRDQTILRNF"
BASE COUNT          503 a          746 c          634 g          506 t
ORIGIN      
        1 ctttggctcc ctcgtcgtcg ctcttgacct tgaaccttcc cggacaagtc tgatgctgga
       61 caatcttcgg gaattccttg cgcaggcacg aaagaaccca aagatcaccc tgctccatgc
      121 actcgctccc cagtccgcgg aggacgctcc cgaagttctc aatgccggcc ggcaagctct
      181 tgaagaggtt cgtgacgaaa tctcctcctg ggggtatgaa acctcagcgg agatcgtcag
      241 cggcgatccg tcgaaggagc ttccggcgag aatacgggaa ctcgcgccgt cgctccttgt
      301 catcgggctc cctgttgcgg gcgagctggg agagctcgtc ttcgggagca ccgcggagat
      361 gcttttcgcg ggaacgacct gtcctctgct tgtgttccct ctctaagagc gtgcatatct
      421 tccgccgcgc ttaaacggga agtggcagaa gagcttccat gaagagcgcg tcttcaccgt
      481 cgaggtaata ccccggaacg acatagagcg gagaaaagtc gaatcccccg taaaaagtac
      541 ggctttcttc gtgggaaact cgaacatgca ggtatattct ggagtatccg agggagagcg
      601 caacctcgga tactccgagt acaagctggg aacctattcc ctttctcctg tattcaggga
      661 gcaccgcgat gttcgtgagc tcagtccctt tctcacaagg cccgagagcc gcgaaaccca
      721 gcaaaatatc cttaccccac gccccgaggt acagcatcgg cgtgtctccg gccaaatcgc
      781 gccggatgag agttcgaggc caaggagcaa ccgacgagcg ctcctccaca aaacagacgt
      841 ccggcgcatc cttgagagaa cagtatcgaa tatcagccag gaaaattgga atcccttctc
      901 ttcgtcagaa attcctgaga atcgtctgat cccttccggg gccgacgccg atgagattca
      961 ccggaactcc ggtcgcggac tcgatatgct ccacgtactt ccgggcctgc ggaggaagct
     1021 gttcgtacga cgtgcaggaa gcgatgtcct catgccagcc ttcgaggtat tcgtagtgcg
     1081 gagaggcttt ctcaaggcgt tgaagagcgc agggaaagtg aggctcatcc tgtccttcca
     1141 ccatatatga agtgcacacg gggattcgtt cctgaccggt gagcacatca agttttgtca
     1201 aggcgatcga ggagatgccg ttcaccctga ccgcatagcg cagcgccaca aggtcaagcc
     1261 acccgcagcg gcgcgggcgt ccggtcgtcg cgccgaattc cgcacccttc tcccgcagct
     1321 tgtttccttc gtcgccgcaa tcctccgtgg ggaaaggccc ctcgccgacc cgggtgcagt
     1381 aggcttttac aacgccaata acccgcccca ccatcgaagg gccgactccc aggccgacac
     1441 agcctcccgc cgcaacagga gaagaactcg tgacataggg ataggttccg tgatcaatat
     1501 cgagcagcgt cccctgcgcg ccctcaaaaa gcacgccttt gccctcccga agggcctcgt
     1561 cgatgatcag cgaggcgtcg ccgacgtacg gcgcgagcga ttttccccat gagagcgcga
     1621 tcgagtacat ttcatgaaac ggaagaggct cctcgccgta tattttggag aggtacgtat
     1681 ttttctcgtc aaggttgtat tccagcttct ctctgaggac gtcgccgtcc aggagatctt
     1741 ccacccggat tccgcagcgg ctgtacttgt cgacgtacgc tgggcctatt cctctcccgg
     1801 tcgtgccgat tttcctcccc ttgccccgaa gctcttcctg ggccttgtcc agcactttgt
     1861 gatagggcat cacgacatgc gccgcgccgc ttatcaccag ccgggcgcgg tctttcccct
     1921 ggtcctgaag atccttgagc tctttcagaa gctgctcggg gtcgatgacc accccgttgc
     1981 cgatgatgca aatcttgcac gggtacaaca tccccgacgg caaaaggtgg aagacgtatt
     2041 tctgcccctc gacgattact gtatgccctg cattcgcccc tccctggtag cgggcgaaaa
     2101 tatcgacctt tgctccaagg gcgtcaacaa cccgtccttt tccttcatcg ccccactggg
     2161 cgccgataat aacctcgact cttcctttca cagtacagcc tcctcaggta gtgccacgtt
     2221 cttgcggaaa aaatccgcgc tgctcctctc gcttcacaag caatcagcgt atccggagca
     2281 gatggaacaa atccggaagg gagacgaata ggtagccgtc cggaagcgct ccttccaact
     2341 tttgcagata ttgcagcgtc tcgatcctcg tgtgcgcaat gacgacaac
//
LOCUS       DAOPKU010000476         2384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_51593_length_2384_cov_4.288965, whole genome shotgun sequence.
ACCESSION   DAOPKU010000476 DAOPKU010000000
VERSION     DAOPKU010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51593_length_2384_cov_4.288965"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1759)
                     /locus_tag="PK849_12470"
     CDS             complement(116..1759)
                     /locus_tag="PK849_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE66987.1"
                     /translation="MRRILPALLDTPPDRTLEKVKIADLRLDFSMFLHRLFLLSRRPD
                     EGAEIVVMALATDKKPDRSVPGLMRYMDYLRARRGILTNGRELRVYEQLACGESELLI
                     QCTEDEIPARTNELRTVLNVASCAEETPPGGEEHRPADDAPESVVLSVESEGAALHQE
                     ASVLPKNAEEPLNTPEPTLQELDVEPDEEESESTAPETLSAAFSEELNAAETAEEGDD
                     DAPAQDSPFQPIESEALLPEPAARDDAPVCPEHKGRMKIIAVYHNKGGVGKTTISVNL
                     AAALRKKGRRVLLIDMDAQANATFAAGLVKFQFEEEDDIIQKYVYHLLGSGDFDFIPD
                     VVRKSKSFNTPEIDIVPSHIDLTNHQHKLTQITASRSRMIMKLRRVQNDYDYVIIDTP
                     PSRDLYAEIPLATADYLIIPSDLRPFANQGLNNVKHFVREVNEYRESIAREPLVVLGV
                     LPSKIPTNAQYLKHSFPRQKMVIPERYGFPLMDSVIYERMPLAASLSRTRKEDDMDIP
                     DPKSIFEFCAMESAASAGQAAEDFELLADEILLKTGWRE"
     gene            2058..2363
                     /locus_tag="PK849_12475"
     CDS             2058..2363
                     /locus_tag="PK849_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66988.1"
                     /translation="MLREESLRFIRALAEEYGAARVFLFGSCLLLPQEDARDIDLAVE
                     GISEEQLDAFWDRLMWADALGRKPVDVLRLEDGHWLNPIILDEGVLIYEAQLSPIAL"
BASE COUNT          484 a          650 c          693 g          557 t
ORIGIN      
        1 tccgtcgctc tccaaaatcg agtcggccaa actttcgacg gcttcggggt cgaaacggtc
       61 gcggggctgc gctgaaacga tgcttgcaac atccacaaga aagtgcttca ttctcttatt
      121 ctctccatcc tgtctttaaa agaatttcat ccgcaaggag ttcgaaatcc tccgccgcct
      181 gtcctgcgga tgcggcggac tccatcgcgc agaactcgaa gatcgacttc gggtccggaa
      241 tatccatatc gtcttccttg cgcgtcctgc tcaacgaagc cgcaagcggc atcctctcat
      301 agatcacgct gtccatcaag gggaagccgt accgctcggg tatcaccatt ttctggcgcg
      361 ggaacgagtg cttgaggtac tgcgcgttcg tcggaatctt cgacggcagc acgcccagca
      421 cgacaagcgg ctcgcgggct atggattcgc ggtactcgtt cacctcgcgc acgaagtgct
      481 tcacgttgtt cagtccctga ttcgcgaagg ggcgcaaatc ggaggggata ataagataat
      541 ccgcggtggc cagcgggatt tcggcataca gatcccttga aggaggcgtg tcgatgatca
      601 cataatcgta gtcgttctgc acccgtctga gcttcatgat catgcgcgac cggcttgccg
      661 tgatctgcgt cagcttatgc tgatggttcg tcaggtcgat gtgcgaaggg acgatgtcga
      721 tctccggcgt attgaaggat ttcgacttcc ggaccacgtc ggggataaaa tcgaaatcgc
      781 cagatccgag aaggtggtag acgtatttct ggatgatatc gtcctcttcc tcgaactgga
      841 atttcacgag tccggcggcg aacgtcgcgt tcgcctgggc gtccatgtcg atcagcaaca
      901 cccggcgccc tttctttcgc agcgctgcgg caagattgac ggaaatagtg gttttcccta
      961 cgccgccctt gttgtgatag accgcgatga tcttcatgcg ccctttgtgt tccgggcaga
     1021 ctggggcatc gtcccgtgcc gccggttccg gaagaagcgc ttcgctctcg atcggctgga
     1081 acggcgagtc ttgagccgga gcgtcgtcgt ctccttcttc cgctgtttcc gctgcattca
     1141 gttcttcgga aaacgctgcg gagagcgtct cgggcgccgt cgattcgctt tcctcttcat
     1201 cgggctcgac atcaagctcc tgcaaggtcg gttcgggtgt attcaaaggt tcctcggcgt
     1261 ttttgggaag aacggaagcc tcctgatgca gagcggctcc ctcggattca acagaaagaa
     1321 caacgctctc cggggcatcg tccgccggac gatgttcttc gcccccggga ggcgtttctt
     1381 cggcgcatga agcgacgttc aaaacggttc gcagctcgtt tgtccgcgcc gggatctcat
     1441 cttcggtaca ttggatgagc agctcgcttt ctccgcaggc gagctgttcg tacacccgca
     1501 actcccggcc gttcgtcaga atgccgcggc gggcgcggag atagtccata taccgcatca
     1561 gcccgggtac ggagcgatcc ggtttcttgt ccgtggcgag agccatgacg acaatctcgg
     1621 ctccttcatc cggccgtcgg gagagaagga aaaggcggtg caaaaacatg gagaaatcga
     1681 gccggagatc ggcgattttc accttttcaa gagtcctgtc cggaggagta tccaaaagtg
     1741 cgggtagtat gcgtctcacg aagcgtgtgc gtacttcttt tttatttcgg tacattccga
     1801 tccgactgtc caaacagctc tccccccgta ccataaaatt ccggtgttgt tggagcatac
     1861 tgtataatac catttttctc aaggagagcg cggaggctcg gagctccgga aaatccggag
     1921 ggcccgagct atctcggtca cgttcaggaa aacacggacg gaaaacgctt tatgttatca
     1981 ttggatacat cggaacatca gttatcaggt gggtgttttg ccggagtgcg aagtgtgatg
     2041 aaagcgagcc gatgaatatg ctgagagaag aaagcctgag gttcatccgt gcgttggcgg
     2101 aggagtacgg ggcagcccgc gtttttttgt tcggctcctg cctcttgctg cctcaggagg
     2161 acgcgcgcga tattgatctc gcggtggagg gaatctccga agagcagctg gatgcgtttt
     2221 gggacagact gatgtgggcg gacgcgctcg gacgcaagcc ggtcgatgtg cttcgtctgg
     2281 aggacgggca ttggctgaac cctataatcc tggacgaagg agtcctcatc tatgaagccc
     2341 agctatcgcc aatcgctcta tagactgctc gaaaagaaca gaga
//
LOCUS       DAOPKU010000477         2384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_51604_length_2384_cov_3.228854, whole genome shotgun sequence.
ACCESSION   DAOPKU010000477 DAOPKU010000000
VERSION     DAOPKU010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51604_length_2384_cov_3.228854"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PK849_12480"
     CDS             complement(<1..573)
                     /locus_tag="PK849_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66989.1"
                     /translation="MQIFKERRVQILAFFIVLLFAAYVVAQSLDVLSLNEVAVSTHSQ
                     ELEGIQGATITATEVQVTKAVKRGKNKDAQTLDSLMKKQMECFKIINAALGAQQSYYS
                     AQTGKDTAKAKAALSSLRTAIPKAQSALETLRGVTQKKIELLRASTGDIGAINVENAT
                     FDSWAAAVRSLSTDLLTEKQVTEKNQIIAEN"
     gene            complement(900..2078)
                     /locus_tag="PK849_12485"
     CDS             complement(900..2078)
                     /locus_tag="PK849_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HPE66990.1"
                     /translation="MSEIVRTNGKILEEHLGELVRSTVEETLNGLLDAEADHICGAER
                     YERSPDRMDSRSGHYQRKLKTKAGEVKLKVPKLRKLPFESAIIERYRRRESSVEESLV
                     EMYLAGISVRRVESITEALWGERVSPGTISNLNKKIYGRIEEWRNRPIEGEHPYVFLD
                     GVWLKMSWGGEVQGVSVLVAIGVNTEGYRDVLGVCLGSREDEGSWISFLRHLKERGLK
                     GVRLVTSDKASGLIGLIGNFFPGATWQRCVVHFERNVLKDVPTNKAEEVGRMLRAIFS
                     QESRESALEKAASVVIRLKDMKLQRAAKTLEQGIGETLSYYDFPAVHWRKIRTNNALE
                     RLNREIRRRTNVVGSFPDSEAALMLVAARLRYVLSHQWGTKRYMNMEPLLEREIESAI
                     "
BASE COUNT          567 a          658 c          549 g          610 t
ORIGIN      
        1 gttttccgcg attatttgat ttttttcagt cacttgtttt tccgtaagaa ggtcggtcga
       61 aagcgatcgc acggctgcag cccacgagtc gaatgtagcg ttttcgacat tgatcgctcc
      121 aatgtctccc gtactcgcgc gtagtagctc tatcttcttc tgcgttactc ccctgagtgt
      181 ttcgagcgca ctctgtgctt taggaatcgc agtccggaga gaggaaagag cagcctttgc
      241 ctttgcggta tcctttcccg tctgagcgct atagtagctt tgctgcgctc ccagtgcagc
      301 attgataatc ttgaagcact ccatttgctt tttcataaga ctgtccagag tttgcgcatc
      361 cttatttttg cctctcttga cggccttcgt cacctgaacc tcagtcgccg tgatcgttgc
      421 tccttgaatt ccttccagtt cctgagaatg cgtgcttaca gccacctcat tgagcgacag
      481 aacgtccaat gactgtgcaa ccacgtacgc agcaaagaga agcactatga aaaaagctag
      541 aatttggaca cgccgctctt taaaaatttg catgcaaaaa ctccccttta acaagtatta
      601 aagtataaag aacaaaaaga ttaataggac aacacaatcc acatgagagc tcacgataaa
      661 aacaagcaag cactcaaatg aaattttaaa gagcaataga ctaaaggaat ggataattat
      721 acctgttcac caaaaggcct cctttgagct tggaaacatg acatctcatt gaccataaaa
      781 agtcaaaaaa acgcatgcca cataaggtag tgtcaagagt ttttcgcaaa agttgaattc
      841 agacaatagc accttgaaaa tcggtcctgc tgtagcttac ccatagtatg catttcgtcc
      901 tatatggcgg attcaatttc tctttccaac aacggctcca tgttcatgta ccgctttgtc
      961 ccccactggt gtgagagcac atatcgaagt ctggccgcca caagcatcaa tgccgcttcg
     1021 ctgtccggaa agcttccgac gacgttggtt ctcctccgga tctccctgtt caatcgctcg
     1081 agggcgttgt tggtccgtat ctttctccag tgcaccgcag ggaaatcgta gtagctcaat
     1141 gtctctccga ttccctgttc aagagttttg gcggctcttt gcagcttcat atccttgagg
     1201 cggatcacga cgctcgccgc cttctcgaga gcgctttcac gggactcctg cgagaagatc
     1261 gcccgaagca ttcgaccgac ttcttccgcc ttgttcgtcg gaacgtcctt cagcacgttc
     1321 cgttcgaagt ggacgacgca ccgctgccag gttgctccgg gaaagaagtt cccgatgaga
     1381 ccgatcagtc ccgaggcttt gtccgaggtg acgaggcgaa ctcccttcaa tccgcgctcc
     1441 ttgaggtgac gaaggaagga gatccagctc ccttcatcct cccgagaacc gagacaaacg
     1501 ccgagaacgt cgcggtagcc ttccgtgttc actcctatgg caacgaggac ggaaacgccc
     1561 tgaacctcgc ctccccaact catcttgagc caaacgccgt cgagaaagac gtacggatgc
     1621 tccccctcaa tgggacggtt gcgccactcc tcgatccggc cgtagatctt tttgttcagg
     1681 ttgctgatcg tccccggact gacgcgctcg ccccacaacg cctcggtgat gctttcgacg
     1741 cggcgcacag agatacccgc caggtacatc tcgacaagag actcctcgac ggagctctcg
     1801 cggcggcggt agcgttcgat gatcgccgat tcgaacggca gcttgcgaag cttgggaacc
     1861 ttgagtttca cctctccggc tttcgtcttc agctttcgtt gatagtggcc ggaacggctg
     1921 tccatcctgt cgggacttcg ttcgtagcgc tccgcgccgc agatgtgatc cgcttcagcg
     1981 tcgagcaatc cgttcaaggt ctcttcaact gtagaccgga ccagctcccc gaggtgttct
     2041 tcaaggatct ttccgttcgt gcgaacgatc tccgacattg cttcttccag aacttcgggc
     2101 gctgtggtac gattctccat gacgacaatc ctccttgggc tctcgctggg gtcagataca
     2161 gcaagaatac ccgatgtaag ggttgtcgtc atctccttca aaccattttg cgaaagatat
     2221 tatacgttat cccacataat ctgtcccaaa atatcgaaca atatgttttc ctatcaacaa
     2281 agccccggtg aaacaaaaag ttcggtgacc ggatatttaa aaaatatggg tcaccttgga
     2341 atgagggata acgtataata tctttcgcaa aaaggtttga agga
//
LOCUS       DAOPKU010000478         2381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_51685_length_2381_cov_4.622958, whole genome shotgun sequence.
ACCESSION   DAOPKU010000478 DAOPKU010000000
VERSION     DAOPKU010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51685_length_2381_cov_4.622958"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="PK849_12490"
     CDS             complement(<1..1645)
                     /locus_tag="PK849_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPE66991.1"
                     /translation="MSRREILGVRIKLASPERIRELSSGEVKKPETINYRTLRPEKDG
                     LFCERIFGPTKSFECACGKYKRSGPKFKGIVCDRCGVEVTDNRVRRERMGHIELAAPV
                     VHIWYLRGIPSRLSLLLGCTTKDLEKVVYFAPTRQREQAYKVIMEGRRTDLARKGDLI
                     SESEVRIHTHYDPKFKAEEAYRILTVDDLPVNEGDVVSANQVSRYKTEYGDELFKVET
                     AFKLVSAPEGSLFSDGDIVSASEIERMKERFGDDAFEKERAYIGNAEAFIVTAAVHLP
                     FAKGDMLSDNECRSYNQKYPGRFTAQKEAITVEDPCYLVIEPGLSPYMYADIIIEREY
                     DLCVAYDKKFSAGIGAEGVQALLSIMDMEQLVAALREAVSESAGQKKRKLIKRLQVAE
                     DFRKGNAAPNAMILEVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLQ
                     ELKAPEIIIRNEKRMLQEAVDALIDNGRMGKAVLGAGNRPLKSLTDLLRGKKGRFRQN
                     LLGKRVDYSGRSVIVIGPHLKIYQCGLPKQMALELFKPFV"
     gene            complement(1645..>2381)
                     /locus_tag="PK849_12495"
     CDS             complement(1645..>2381)
                     /locus_tag="PK849_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPE66992.1"
                     /translation="VFEGANEQEIFDLLNDVAKEKYPELTSDGMITLYDGRTGDPVEK
                     KVTVGCMYMLKLIHLVDDKIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEGY
                     GAANILQEMLTVKSDDIRGRLKTYERIVKGQNLTKPGVPESFRVLVKELQGLGLDVEV
                     KYSDGVIGELVMDEDEDDVVGSRRAPRERSILQEEPEAEVEKEVVEKEKFFEDEDEVG
                     GIVPEEMLFGGALPEGMSIEEEGDDN"
BASE COUNT          389 a          751 c          702 g          539 t
ORIGIN      
        1 tgacgaacgg cttgaagagc tcgagcgcca tctgcttcgg cagtccgcac tggtagatct
       61 tgaggtgcgg gccgatgacg atgacggagc ggccggagta gtcgacgcgc ttgccgagca
      121 ggttctggcg gaagcgtccc ttcttgccgc gcaaaaggtc ggtaaggctc ttcagagggc
      181 ggtttcccgc gcccaggacg gccttcccca tacgcccgtt gtcgatgagg gcgtccacgg
      241 cctcctggag catgcgtttc tcgttccgga tgatgatctc cggcgccttc agctcctgga
      301 gcttcttcag gcggttgttc cggttgatga ccctccggta gaggtcgttg aggtcggagg
      361 tggcgaagcg gcctccgtcg agctgcacca tggggcgcag gtcgggcggg atgaccggga
      421 gcacttcaag gatcatcgca ttcggcgcgg cgttcccctt gcggaagtcc tcggcgacct
      481 ggaggcgctt gatgagtttg cgcttcttct gccccgcgct ctccgagacc gcctcgcgga
      541 gcgcggcgac gagctgttcc atgtccatga tcgagaggag cgcctggaca ccctccgcgc
      601 cgatgcccgc ggagaacttc ttgtcgtagg ccacgcagag gtcgtactcc cgctcgatga
      661 tgatgtcggc atacatgtag ggcgagagcc ccggctcgat gacaaggtag cagggatcct
      721 cgaccgtgat ggcctctttc tgggcggtga aacggccggg atacttctgg ttgtacgatc
      781 tgcactcgtt gtcggagagc atatctccct tggcgaaggg aagatgcacg gcggcggtga
      841 cgatgaacgc ctccgcgttg ccgatatatg cgcgctcctt ttcgaaagcg tcgtccccga
      901 aacgctcctt cattcgctct atttcgctcg cggagacaat gtcgccgtcg gagaaaagcg
      961 agccctccgg cgccgaaacg agcttgaacg ccgtctccac tttaaagagt tcgtcgccgt
     1021 actcggtctt gtaacgggag acctggttgg cggagacgac gtccccctcg ttcaccggga
     1081 gatcgtccac ggtgaggatc ctgtaggctt cctccgcctt gaatttgggg tcgtagtgcg
     1141 tatggatgcg aacttcgctc tcggagatga ggtcgccctt cctcgcaaga tcggttcgcc
     1201 gcccctccat gataaccttg tacgcctgct cgcgctggcg ggtgggcgca aagtagacga
     1261 ccttttcgag atccttcgtg gtgcagccga gcagcaggct cagtctgctg gggatgccgc
     1321 ggaggtacca gatatggacc accggcgcgg caagctcgat gtgccccatc cgctctcgcc
     1381 ggactctgtt gtcggtaacc tcgacgccgc acctgtcgca gacgatcccc ttgaacttgg
     1441 ggccgctgcg cttatatttg ccgcaggcgc attcaaagct cttcgtaggg ccgaagatac
     1501 gctcgcagaa gagcccgtcc ttttccggcc ggagcgtgcg gtagtttatc gtctcgggct
     1561 tcttgacctc tccgctggag agttcgcgaa tccgctcggg gctggcgagc ttgatccgca
     1621 cgcccagtat ctctctacgg ctcatcagtt gtcatcccct tcttcttcga tggacattcc
     1681 ttcgggaagc gcccctccga acagcatttc ttcgggaaca atccccccga cttcatcctc
     1741 gtcttcgaag aatttctcct tctccacgac ttccttctcg acctcggcct ccggctcctc
     1801 ctggaggatg ctgcgctctc tcggcgcacg gcgggatccg acgacgtcgt cttcatcctc
     1861 atccatcaca agctctccga taacgccgtc gctgtacttc acctcgacgt cgagccccag
     1921 cccctgcagt tccttcacaa gcactctgaa actctcggga actcccggtt ttgtcaggtt
     1981 ctgtcccttg acgatgcgtt cgtaggtctt gagacgcccc ctgatatcgt ccgacttgac
     2041 ggtgagcatc tcctggagga tattcgccgc gccgtaccct tcaagcgccc agacttccat
     2101 ctctccgaat ctttggccgc cgaactgcgc cttgcccccg agaggctgct gggtgatgag
     2161 gctgtacgga ccgatcgagc gcgcgtgaat cttgtcgtcg acaaggtgta tcagtttcag
     2221 catgtacatg cagccgacgg tgaccttctt ttccacaggg tcgccggtgc ggccgtcgta
     2281 gagcgtgatc atgccgtcgc tcgtgagctc gggatacttt tccttcgcaa cgtcgttcag
     2341 caggtcgaaa atctcctgct cgttcgcgcc ctcgaagacc g
//
LOCUS       DAOPKU010000479         2381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_51695_length_2381_cov_3.369304, whole genome shotgun sequence.
ACCESSION   DAOPKU010000479 DAOPKU010000000
VERSION     DAOPKU010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51695_length_2381_cov_3.369304"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PK849_12500"
     CDS             <1..697
                     /locus_tag="PK849_12500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPE66993.1"
                     /translation="YGIIYSTNAQIMGASYVTKKHLYFVRHGKTEWNGQYRFQGKSDI
                     PLNEEGRLQAQSLASRLSGWSGVRILTSPLSRALETSAIIAAACGAVAEPQEGLSEMG
                     FGAWEGRSIFETETSDPQAFREWKNNPFKFVPPGGEGFPEIQGRLLPVLDEALSSVQE
                     RILLISHGGIIRAALSLLLDLSQESVWRMKLSNCSVTAVESGKRGKSLLFLNDDIHTV
                     LPAVFLPNLPFSS"
     gene            758..1402
                     /locus_tag="PK849_12505"
     CDS             758..1402
                     /locus_tag="PK849_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPE66994.1"
                     /translation="MTGKATSAEKRDASLPQESAMVLPDTAGGSPEAMDEATLWRRCA
                     EDHDEDARDSLIVSYRPLVFWLAKKFHVHPSSYQDLIQEGMLALIRAVDKFEPERHLK
                     FTTYAFYRIKGQMINFLQRSEKKAPLPVDDEFLMPEDPFLPETFETMLALSEELRRLP
                     AKEGEVLQAILMDGREAREVAQERGIDISHVYRLKRNALAKLRKWLGAEATNEV"
     gene            1483..2241
                     /locus_tag="PK849_12510"
     CDS             1483..2241
                     /locus_tag="PK849_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE66995.1"
                     /translation="MEKKIARIYRWLERCAKACSAGSWNSALLDMECAKAELEDVRTE
                     IWSKTEGAFEVRPTRRGALALKASFLSVVLLFSLAAPLAIQSSRPASSVYQASGNVHS
                     GEHVLEWVTGDEQAVLAALRKSLSDANRAWSEAALRDETDAVAGEAASGPAMNEESLE
                     EEKTYVARAPSASEGSGASRESGAETVSRRVRPRGQGEPAAGSAGLKNEVVAGEDAIR
                     PSSAANELDSIISLVQIGQKALRESESPIQFQRP"
BASE COUNT          540 a          588 c          697 g          556 t
ORIGIN      
        1 atatggtatt atttactcca caaatgctca aatcatgggg gcttcgtacg tgactaaaaa
       61 acacctttat tttgttcgcc atggaaaaac tgaatggaac ggacaatatc gtttccaagg
      121 gaaaagcgac atccctctga acgaagaggg gcgtctgcag gcgcaaagcc ttgcttcgcg
      181 cctcagcggc tggagcgggg tgagaatact cacgagtccg ctctccaggg ctcttgagac
      241 atccgccatc atcgccgcgg cctgcggggc ggtcgctgaa ccgcaggaag ggctctcgga
      301 gatgggattc ggcgcatggg aagggcgctc gatctttgaa acggaaactt cggacccgca
      361 ggccttcaga gagtggaaga acaatccctt caaatttgtc cctccgggcg gggagggttt
      421 tccggagatt caaggtcgcc ttcttccggt gctcgatgaa gccctctcct ctgttcagga
      481 gcgaatcctt ctcataagcc acggagggat catccgcgcc gctctttccc tccttcttga
      541 tctgagccag gagtccgtgt ggaggatgaa actgtccaac tgttcggtga cggctgttga
      601 atcgggaaag cgcggcaaat cccttctctt tctcaatgac gacattcata ctgttttgcc
      661 cgctgtattt ttaccgaatc tccctttctc ctcttagaca agaggcgctc ttgctggtag
      721 aatttactgg aattcgatac gagggagtgt gcatgccgtg accgggaaag caacatctgc
      781 agaaaaacga gacgcaagtc tccctcaaga gagcgcgatg gttcttcccg atacagccgg
      841 aggctcccct gaagcaatgg atgaagcgac gttgtggagg cgatgtgcgg aagatcacga
      901 tgaagatgcg agggattccc tgatcgtatc ctatcgccct ctggtattct ggctcgcaaa
      961 gaaatttcac gttcacccct cgtcgtacca ggatctgatt caggagggga tgcttgccct
     1021 cattcgtgcg gtggacaagt tcgagccgga acgtcatctc aaatttacca cctatgcgtt
     1081 ttaccggatt aaaggacaga tgatcaactt tctgcagcgc tcagaaaaaa aggctcctct
     1141 tcctgtggac gatgagttcc ttatgccgga ggaccctttc cttcctgaaa cattcgagac
     1201 tatgcttgct ctttcggaag agttacgacg gctcccggca aaggaggggg aggtgctgca
     1261 ggcaattctt atggatggac gggaggcaag agaggtagcg caggagcgcg gaatcgatat
     1321 cagtcacgtc tatcgtctga agaggaatgc gctggcgaaa cttcgcaaat ggcttggcgc
     1381 cgaggccaca aacgaggtct aaaacgggat aagaaaatac agtgatcgcc cacgtacatt
     1441 ttttgtttga aagcgtggag tacattcccg ggaggacttt ctatggaaaa gaagatcgct
     1501 cgaatttata gatggcttga acgatgcgcg aaagcatgtt ccgcaggttc ctggaacagc
     1561 gcgctgcttg atatggagtg cgcgaaagcc gaactggaag atgtccggac ggagatctgg
     1621 tcgaagaccg agggggcttt cgaggtgcgg ccgaccagga gaggcgctct cgccctgaag
     1681 gcgtcttttt tgagcgtcgt ccttcttttt tctcttgccg cgccgcttgc gatacagagt
     1741 tcgagaccgg cgtcttctgt gtatcaggcg agcggaaatg ttcactcggg agagcatgtc
     1801 cttgaatggg tgactggcga cgagcaggct gttcttgccg ctcttcgtaa aagcctgagc
     1861 gacgcgaatc gagcatggag cgaggctgcg cttcgagatg aaaccgatgc ggttgctggt
     1921 gaagcggcga gcggccccgc catgaacgaa gaatcgcttg aggaggagaa aacctatgtc
     1981 gcccgcgctc cgtctgcgtc ggaaggaagc ggagcttccc gcgagtccgg ggcagaaact
     2041 gtttcccgcc gggtgcgtcc aagaggtcag ggagaacctg cggcaggctc agctggactg
     2101 aagaacgaag tcgtcgccgg tgaagacgct atccgccctt cctcggcggc aaatgaactc
     2161 gattcaatca tctcgcttgt gcagatagga caaaaagccc tgcgcgagag tgaatccccg
     2221 atacagtttc aacgtccgtg atgcaaacgg ccggttgttt tttacgggct tctgattttg
     2281 ttttgttgat ggcgtgcgtt tcactgagtg tgtttctttt cgtcctgttc aggacgaatt
     2341 catttttgtt ttttggaagg agagagcgtg cgtgaaagga a
//
LOCUS       DAOPKU010000480         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_51809_length_2377_cov_4.957795, whole genome shotgun sequence.
ACCESSION   DAOPKU010000480 DAOPKU010000000
VERSION     DAOPKU010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51809_length_2377_cov_4.957795"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="PK849_12515"
     CDS             complement(<1..1773)
                     /locus_tag="PK849_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE66996.1"
                     /translation="MAFRKMWLKINGANRIFICDPEKDSLADVLRRLGLTSVKIGCGT
                     GVCGSCSVILDGALVRSCTRKIGKIPEYSEVITLEGIGTPDHLHPLQVAFMKLGAVQC
                     GFCTPGFIVSSYALFLENKNPTRKEVRTWFQKHKNVCRCTGYKQIVDAVMEAAKVVRG
                     EARIDEIGPSYSTEDGYYGKPLVRPTALAKVCGLADYGDDQELKMPAETLHVAMVQPR
                     VAHHAKILEIDISEAEKMPGVAKIILAADIRAAGGTNIMAEGQMHERTTVMAPSREVL
                     NEKKIFRYGDVVALAVADTKDHARAAAAKVKVTIEQLPEYLNFLDAVMPDAMRIHEDT
                     PNIFCIQPLLKGAGLEDASKVAGIIDSSPYSAEGSFYSARQPHLTIEGDSIQAYFDED
                     GYLTFQCKSQGVYSSIGRIGNSIGMPKDKLRVVMNPTGASFGWSTNAGDLCLAGAAAM
                     VMKAPVSLSMSYEEHQHFSGKRCPCHSNGRAACDENGKITAVEFDFGMDHGAYSWGGD
                     DIVTKQPRFAFFPYYVPHAAGLARIANTNHTFGTAYRSYGSPQAYTLSEALMDMLAEK
                     AGIDPFEFRWRNIAREGQTNINSYP"
     gene            complement(1804..2127)
                     /locus_tag="PK849_12520"
     CDS             complement(1804..2127)
                     /locus_tag="PK849_12520"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPE66997.1"
                     /translation="MDVFKAVDMAVKGNIYEGGKVTSRTFWTADGERKTVGIMLPGSY
                     SFSTSTQEKMEMTNGIVEVRLDPDGEWSRYKEGEFFMVPAGVTFEIRCAGVAEYVCSY
                     LNESK"
BASE COUNT          408 a          700 c          729 g          540 t
ORIGIN      
        1 gggatagctg ttgatgttgg tctgtccctc gcgggcgatg tttctccagc ggaactcgaa
       61 cgggtcgatg ccggcttttt cggcgagcat gtccatcagc gcctcgctca gcgtgtaggc
      121 ttgcggggaa ccgtagctcc tgtaggccgt gccgaaggtg tggttcgtgt tggcgatgcg
      181 ggcaagcccc gccgcgtgcg gcacgtaata agggaagaag gcgaaacggg gctgtttggt
      241 gacaatgtcg tcgccgcccc aggagtacgc gccgtggtcc atgccgaaat cgaactcgac
      301 cgccgtgatc tttccgtttt cgtcgcaggc ggcgcgcccg ttcgagtggc aggggcagcg
      361 ctttccggag aagtgctgat gctcctcgta gctcatcgag agggaaacgg gggccttcat
      421 gaccatggcc gccgctccgg cgaggcaaag gtctccggcg ttggtggacc agccgaagct
      481 ggcgcccgtg gggttcatca ccacgcgcag cttgtccttc ggcatgccga tggagttgcc
      541 gatgcgcccg atggaggagt agacgccctg cgacttgcac tggaacgtca aatagccgtc
      601 ctcgtcgaaa taggcctgta tactgtctcc ttctatggtg agatgaggct gtctggcgga
      661 gtagaagctc ccttcggcgc tataaggaga ggagtcgata atcccggcga ccttcgaggc
      721 gtcttcaagc cccgcgccct tgaggagcgg ctggatgcag aagatgttcg gggtgtcctc
      781 gtggatgcgc attgcgtcgg gcatgacggc gtcgaggaag ttgaggtact ctgggagctg
      841 ttcgatcgtt accttgacct tcgcggctgc ggcgcgggca tggtctttcg tgtccgccac
      901 ggcgagggcg acgacgtcgc cgtaccggaa gatcttcttc tcgttgagca cttcgcggct
      961 cggcgccata actgtggtgc gctcgtgcat ctgtccctcg gccatgatgt tcgtcccgcc
     1021 cgcggccctg atgtcggcgg caagaatgat cttcgccact ccgggcatct tttcggcctc
     1081 ggagatgtcg atttcgagga tcttcgcatg gtgcgcgacg cggggctgca ccatggcgac
     1141 gtggagcgtc tccgcgggca tcttcagctc ctgatcgtcg ccgtagtccg cgaggccgca
     1201 caccttcgca agcgccgtcg gccggacgag aggctttccg tagtacccgt cctcggtcga
     1261 gtaggagggg ccgatctcgt cgattcttgc ctcgccgcgg acgaccttcg ccgcttccat
     1321 gaccgcgtcg acgatctgct tgtagcctgt gcagcggcag acgttcttat gcttctggaa
     1381 ccatgtccgg acctctttgc gcgtcgggtt tttgttctcg agaaagagcg cgtaggacga
     1441 gacgatgaat cccggagtac agaagccgca ctgtaccgcg cccagcttca tgaaggcgac
     1501 ctgcagaggg tgcaggtgat ccggcgttcc gattccttcg agcgtgatga cttcactgta
     1561 ctcgggaatc ttgccgatct ttctggtgca ggaacgcacg agcgctccgt cgaggatcac
     1621 ggaacaggag ccgcagacgc cggtcccgca tccgatcttc acgctcgtca ggccgagccg
     1681 gcgaagcaca tccgccagag agtccttctc gggatcgcag atgaatatcc ggttcgcgcc
     1741 gttgatcttg agccacatct ttcgaaaagc cacagcggta ccccctttat ggtttcttcc
     1801 gtactacttg ctctcattca gataactgca cacgtattcg gcgaccccgg cgcagcggat
     1861 ctcgaacgtg acacccgccg gaaccatgaa gaactctccc tctttgtacc tggaccattc
     1921 tccgtccgga tcgagccgga cctcgacgat gccgttcgtc atctccatct tctcctgcgt
     1981 cgaggttgag aacgaatagc tccccggcag catgatcccg accgtcttcc gttccccgtc
     2041 ggctgtccag aaggttctgc tggtgacctt cccgccctcg tagatgttcc ctttcaccgc
     2101 catgtcgacc gccttgaaca catccacatg aaccagctcc tctcggatag gcgcgcctcc
     2161 ggaagagagg ggcgcttcat agtccactca gtatatgaag agtacgggga gatatgcaat
     2221 attttgatat ttgtgactgc ttgtgtttct ttttggtcgg ttttgctcac ttatgacaac
     2281 gcgcgctttc tcccgtagtg tgtcctgcgc caagtcgggg aagcgttccg gaggaggggt
     2341 gtgtaaagag acggatgcgt tcagaaaaat gtttttt
//
LOCUS       DAOPKU010000481         2375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_51892_length_2375_cov_3.205172, whole genome shotgun sequence.
ACCESSION   DAOPKU010000481 DAOPKU010000000
VERSION     DAOPKU010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51892_length_2375_cov_3.205172"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="PK849_12525"
     CDS             <1..1474
                     /locus_tag="PK849_12525"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HPE66998.1"
                     /translation="FGFSALGFLGLAFLFGAALSAIAQGSTVATSFAIGFVDVGMLSF
                     AGSVVVMMGASVGGTFVTFLLSLDIAAFSPLLFGASFLMVRFGKRTVYRIGCVLQGLS
                     LVLLGMFVLKLGVGPLLQDPRFSDMATVAGSNSLLVGIAAFVATAVLQSSSAVMALAV
                     TLAVSGALPATAILPVVCGAHVGSSVTVLLAGFSGRQNARKLGVCTFVYKFLGVLFIL
                     PFFPWIQGFMASGGGLAAKVVQAQVGVTLFNAVVFYPFTDILAHFSARIAEQAGGERL
                     GAPVYLDDELTDVPSLAILLLSKEMIRLANYIEMYCQVLFAPEKSLEGFEELPFAIRE
                     LSEACQEYMFEIHIPSDDDALRENYSTVSYSMVSFRQMAKILSGELRLLAEAGVAAQF
                     VRELGEKTWRELSRLALTDIRLALRAFALADADFASEAGRYEREFEELDRRIRLRLGV
                     DALNRRELAPLLDFMSQIFLLVKTSLEVARGEARRDREAG"
     gene            1500..2228
                     /gene="phoU"
                     /locus_tag="PK849_12530"
     CDS             1500..2228
                     /gene="phoU"
                     /locus_tag="PK849_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPE66999.1"
                     /translation="MTKNATIQSFFYATDRERIVSKVVLMGERAAQLLENSVSALLDQ
                     DSALARRIIAEDDAIDQMEGEIDQECLGSIAMRSPGKDELYFVFAVLKIITDLERIGD
                     QAVNIAEKALKVTPAVVFPRRDALLKMLRVVLAMFRQALQAFRNEDKHMAATLWKRDD
                     EVDSMYAQCYADFLHDAVAHSGNDDPQTMEIGALELWTARHLERAGDHVINIVERVYF
                     MVEGKYFPSKAARKGSFKIKTSGE"
BASE COUNT          457 a          623 c          718 g          577 t
ORIGIN      
        1 gttcgggttt tcggcgctgg ggttcctggg cttggcgttt ctcttcggcg cggcgctctc
       61 tgcaattgca caggggagca ccgtcgccac atctttcgcc atcggtttcg tcgacgtggg
      121 catgctctcg ttcgccggtt cggtcgtcgt gatgatgggc gcgagcgtgg gaggtacgtt
      181 tgttaccttc ctgctcagcc ttgatatcgc ggcattctcc ccgctgcttt tcggggcgag
      241 tttcctgatg gtgcgattcg ggaagagaac tgtctatcgg ataggctgcg ttctccaggg
      301 attgtcgctc gttcttctcg gcatgttcgt cctgaagctg ggggtaggac ctctcctgca
      361 agatccccgg ttttcggata tggcaaccgt tgcgggaagc aacagccttc ttgtcggaat
      421 agcagctttc gtggcgacgg ccgttctcca gagcagctcg gcggtgatgg cgcttgccgt
      481 taccctcgca gtctccgggg cccttcccgc taccgcgatt cttccggtag tctgcggagc
      541 ccacgtcggt tcgtccgtca cggtgctgct cgcaggcttt tccgggaggc agaacgcgcg
      601 gaagctcggc gtgtgtacgt tcgtctataa gtttttaggc gtcctcttca ttctcccgtt
      661 cttcccgtgg attcagggtt ttatggcttc aggagggggg cttgccgcca aggtggtcca
      721 agcgcaggtg ggcgttaccc ttttcaatgc ggttgtcttt tatccgttta cggacatcct
      781 cgcgcatttt tccgcacgaa tcgccgagca ggcgggaggg gaacgtcttg gggcgccggt
      841 ctatcttgac gacgaattga cggacgtccc ctcgcttgcg attctgctgc tttcaaaaga
      901 gatgattcgt ctcgcgaact acattgaaat gtactgtcag gtactctttg cgccggagaa
      961 aagccttgaa gggttcgagg aacttccctt cgccatacgc gaactttcgg aagcttgcca
     1021 ggagtacatg ttcgagattc atattccgtc cgatgacgat gcgcttcgtg aaaactactc
     1081 cactgtctct tattctatgg tatcctttcg tcagatggcc aaaatcctct ccggcgaact
     1141 gcgtcttctt gccgaggctg gagtggcggc gcagtttgtc cgcgagctgg gggagaaaac
     1201 ctggcgggaa ctctccaggc ttgcgctgac ggatatccgg cttgcgctca gggcttttgc
     1261 ccttgcggac gccgattttg cttcggaagc cggacgctat gaaagagagt tcgaagagct
     1321 ggacaggcgg atacgtctca gacttggagt cgacgccctc aatagacgag agctggctcc
     1381 tctgctcgat ttcatgtcgc agatctttct gcttgtgaag acgtctcttg aagttgccag
     1441 gggagaagcc cgtcgcgacc gcgaagcggg ctgatatctc ggggaggtga agtcgcccgg
     1501 tgacgaaaaa tgcaacgata cagtcttttt tctacgcaac agacagagag cggatagtat
     1561 ccaaggtcgt gttgatgggg gagcgggctg cccagcttct tgaaaactcg gtgagcgcgc
     1621 ttcttgacca ggattcggcg ctcgcacggc ggattatagc cgaggatgac gcgatcgacc
     1681 agatggaagg ggagatcgac caggagtgtc tcggatccat cgccatgcgc tcgcccggta
     1741 aggatgaact gtacttcgtc ttcgcagttc tcaagatcat taccgacctg gaacgaatcg
     1801 gcgaccaggc ggtgaacatc gcggagaagg cgctgaaagt aacgccggca gtcgtcttcc
     1861 cccggcggga cgctcttttg aagatgctgc gggtggttct tgcgatgttc cgccaggcgc
     1921 tgcaggcctt caggaacgag gacaagcaca tggccgccac gctgtggaag cgcgacgacg
     1981 aggttgactc catgtatgcc cagtgttacg cggatttttt acacgacgcg gtcgcccatt
     2041 cgggaaacga cgatccgcag acgatggaga taggcgcgct ggagctatgg acagcccgtc
     2101 acctggagcg agcgggagat cacgtcatca atatcgtgga acgagtctat ttcatggtcg
     2161 agggaaaata cttcccgagc aaggcggcca ggaaaggctc tttcaagata aaaacctccg
     2221 gggaataatc gtttcccgcc tttctttttt ttctgaccaa tattgacaag cctctgaaag
     2281 tgtgtataat gctcttagtc agtattagtc agaaaaacgc aggggggata cgaaatgaaa
     2341 aaggcggtcg gcatagatct tggaacgacg aactc
//
LOCUS       DAOPKU010000482         2339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_52997_length_2339_cov_4.908056, whole genome shotgun sequence.
ACCESSION   DAOPKU010000482 DAOPKU010000000
VERSION     DAOPKU010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52997_length_2339_cov_4.908056"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PK849_12535"
     CDS             complement(<1..710)
                     /locus_tag="PK849_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67000.1"
                     /translation="MAYNVQQFFLQKRGVGYVAPILCTMRGLGESDIEQAVVFHKRIS
                     DEIGDADFFAEENTIAESIMGEGVVVGVFAEGNLIALRAVSYVKEFVDDAVVDLDLDP
                     GEADNVAVMDFCVTDSAFRGNSIQFFTYLFTESLMYPNRYHLHTTVSPKNIFSLTNVL
                     KCGFSAIKFKQKYGGHHRFVLYKNLRKPGAVKTRGHKEILLRNYDYHPKVFADGFVGY
                     KIKHKSNGMAMLYGKPLEG"
     gene            892..1932
                     /locus_tag="PK849_12540"
     CDS             892..1932
                     /locus_tag="PK849_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPE67001.1"
                     /translation="MKNIISPGDSVLLKANMLSASTPEECITTHPEVVRAVAVQTLEA
                     GGKPFIADSPGIDRFSDVARKTGMEAVARELGIPCVELDDPVPLPVAEGNVFQRIEVS
                     RRAVEADTIINIPKLKTHAQMMLTLGVKNLFGTVVSQRKAQWHYNVGLRREAFADLHL
                     DIARGLAPRLTILDGIRGMEGRGPSNGKPRDFGILAASSDPLDLDFRVCTLLGAPLEE
                     FPLYRAARERGLVDKSARWDAPGLPEEFEIPPIELPKLDALHLLPPFLDRFGKRFLAS
                     RPVQEKQKCIRCGKCVEICPAGAMELTKAGDIEIDYEKCIRCYCCQEVCPADAIRFSK
                     GLLLRVLEGLRR"
     gene            2002..>2339
                     /locus_tag="PK849_12545"
     CDS             2002..>2339
                     /locus_tag="PK849_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67002.1"
                     /translation="MKKMLAGSLLALLLGAAFFLFSSCAENRRTPFQQSAPPSAGNGK
                     PAEPALSPEEESAQEISPSPAVSDEPDLLETAPPPSEALEPLSPSPESFLSVPISITA
                     DEAVRILEDAL"
BASE COUNT          468 a          707 c          688 g          476 t
ORIGIN      
        1 ccctccagag gcttgccgta gagcatcgcc attccgttcg acttgtgttt gatcttatac
       61 cctacgaagc catcggcaaa gaccttcgga tggtagtcgt agtttctgag gagtatctcc
      121 ttgtgccccc gcgtcttcac cgcgccgggt ttcctcaggt tcttgtagag cacgaaacgg
      181 tgatgcccgc cgtacttctg cttgaacttg atcgcggaaa aaccgcactt cagcacgttc
      241 gtcaggctga agatattctt cggcgacacc gtggtgtgca gatggtatct gttcggatac
      301 atgagactct cggtgaagag gtacgtaaag aactgaatgc tgttccctct gaaggcggaa
      361 tcggtgacgc aaaaatccat cacggcgacg ttgtccgctt cgccggggtc cagatcgaga
      421 tcgaccaccg cgtcgtccac aaactctttg acataggaga cggccctgag ggcgatgaga
      481 tttccctccg caaagacccc gacgacgacg ccttccccca tgatgctctc cgcgatcgtg
      541 ttctcttcgg cgaaaaaatc cgcgtcgccg atctcgtcgg atatcctttt atggaagacg
      601 acggcctgct ctatgtcgct ctcccccaag ccccgcatgg tgcacagaat gggggcgacg
      661 taccccacgc ctcttttttg aagaaagaac tgctggacgt tgtaagccat cgattcaccg
      721 gcccttccca tgtgtggatg cacttcatcg tttgctgttt cggtgtatat tatacaaaac
      781 gaatcgagag gagagaagat ggtgagcgca acaacattca cacagtgcgg aaaatacagc
      841 cgattggata tcgagaaggc cctcgcggcg ctgttgcaag agttcggggg tatgaaaaat
      901 attatatccc cgggggacag cgtcctgctg aaggcgaata tgctttccgc ctcgacgccc
      961 gaggagtgca tcacgacgca cccggaggtc gtacgcgccg tggcggtgca gacgcttgag
     1021 gcaggaggga aaccgttcat cgcagacagc cccggaatag accgtttctc cgacgtcgcg
     1081 aggaagaccg ggatggaggc tgtcgcacgc gaactgggca ttccgtgcgt cgagctggac
     1141 gaccccgttc cgctgcccgt cgcggaggga aacgtcttcc agcgcattga ggtatcgcgg
     1201 cgcgccgtcg aagccgatac gatcatcaac atcccgaagc tcaagacgca cgcgcagatg
     1261 atgctcacgc tgggggtgaa aaatcttttc ggcacggtgg tcagccagcg aaaggcgcag
     1321 tggcactata acgtcgggct tcggcgggag gcgttcgccg atctccacct ggatatcgcg
     1381 cgcggcctcg ccccacgcct caccatactc gacggaatcc ggggcatgga gggacggggg
     1441 ccaagcaacg ggaagccgag agatttcggc atccttgcgg cctcgtcgga cccgctcgac
     1501 cttgacttcc gcgtctgtac gctgctgggg gcgccgttgg aagagttccc gctctacagg
     1561 gctgcgcggg agcgcggact cgtggacaaa agtgcgaggt gggacgcccc cggtcttccg
     1621 gaggagttcg agattccacc gatagagctg cccaagctcg acgcgctcca cctgctcccc
     1681 cccttcctcg accggttcgg caagcgcttc ctggcgtccc ggcccgttca ggagaagcaa
     1741 aagtgcatcc ggtgcggcaa gtgcgttgag atatgccccg caggcgcgat ggagctgacg
     1801 aaggcgggag acatcgagat agactacgaa aagtgcatcc gctgctactg ctgccaggag
     1861 gtctgccccg ccgacgccat tcgtttttcc aaggggctgc tgctgcgcgt cctggaaggg
     1921 ctccggagat gacgcgaaat ttatccccgc tgcggtataa taacgctacg ttctatccaa
     1981 ctgaggaaag gagatgacgt catgaaaaaa atgctggcag gctcgctcct ggctcttttg
     2041 ctcggcgccg cattttttct gttttcctct tgcgcggaga accgccgaac cccgtttcag
     2101 caaagcgcgc ctccttccgc tggaaacgga aaacccgctg aaccggcgct ttcccccgag
     2161 gaggagtccg cgcaggagat ttccccgtcc cccgcggtga gcgacgagcc ggatcttctg
     2221 gagacggcgc ctcccccctc ggaggcgttg gagccgcttt ctccctctcc ggagtccttc
     2281 ctctccgtcc cgatctccat caccgcggac gaggctgtcc gtattctgga ggacgcgct
//
LOCUS       DAOPKU010000483         2336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_53090_length_2336_cov_4.998685, whole genome shotgun sequence.
ACCESSION   DAOPKU010000483 DAOPKU010000000
VERSION     DAOPKU010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53090_length_2336_cov_4.998685"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1183
                     /locus_tag="PK849_12550"
     CDS             5..1183
                     /locus_tag="PK849_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacylase"
                     /protein_id="HPE67003.1"
                     /translation="MFDYERYKTPRKIVLLLLALALAGFGAREFTELRTMKETVVVSD
                     AFTKMVMLSDYFPPIKGTGVDTPVYIYDSGVPGGSMFYLGGTHPYEPATMLSAYVMME
                     NLKVEKGRVFICPHANMSASTVGMLGNAYPKFLHVETAFGPKPYRIGDRNTSPLDQWP
                     DPFTYVHYPSGQNLDYTDARNLNRTYPGRPDGNLTEQLTFAIMELNRKEKITLFVDAH
                     EASLMYPVVSTYVAHDRALDMAMMASMMLSATEFPMKCEASPKNLRGLSHRDAGDFCD
                     SLVILMETPEPFIDRVAGKITEKLMMDGKDEFLQTAAEHGLLFTDYDISVGCPMWDRV
                     GRHLSGALAMIQQMNDFLMEPEKELQLSFPNYSDLKEHGLGHFLNDPAKADPAKVFLN
                     "
     gene            1296..>2336
                     /locus_tag="PK849_12555"
     CDS             1296..>2336
                     /locus_tag="PK849_12555"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPE67004.1"
                     /translation="MLKSFRTMLCAMLCVILLSSAALACTVLAVGKDATVDGSAMITH
                     NDDSRVANSRLFIIPEADWPEGSMRSVVKDAHGYEGDGQVIGEIPQVPHTFRYFFSRY
                     SFMNEKGVAISESTDSVEVTDERSKKVEQIMAKDAVGMMDAWILQDIMLERASTAREA
                     VEIMGKLVEEYGWYDAPETMPLTDGNEVWIAEFYGNKIWAAWRMPDDHIFVGANRARL
                     RHLDLTDKENVMYCPDLVEFAVKNGFIEEKDVNMKDFSPADVYCPSTALYATRREWRV
                     LSLAAPDSFKLGPDEYDYPMSIKPDKKLSVHDVLRIKGDWYEGTPFDLSKGIQAGPWG
                     NPIRFANSSKKNP"
BASE COUNT          498 a          757 c          672 g          409 t
ORIGIN      
        1 gagaatgttc gactacgaaa gatataaaac gccccggaag atcgtcttgc tgctgctcgc
       61 gctggcgctt gcgggctttg gagcccgcga gtttacggag ctgcgcacga tgaaggagac
      121 ggtcgtcgtc tcggacgcct tcacgaaaat ggtcatgctc agcgactact ttccccccat
      181 caaggggacg ggagtggaca cgccggtcta catctacgac tccggcgtgc ccggcggttc
      241 gatgttctac ctcggcggga cgcaccccta cgagcccgcc acgatgctct ccgcctacgt
      301 catgatggag aacctcaagg tggagaaggg acgtgtcttc atctgccccc acgccaatat
      361 gagcgcctcc accgtcggaa tgctggggaa cgcctacccg aagttcctcc acgtggagac
      421 ggcgttcggc cccaagccgt acagaatcgg cgacaggaac acctcgccgc tcgaccagtg
      481 gcctgatccg ttcacgtacg tccactaccc gtcggggcag aaccttgact acacggacgc
      541 gcgcaacctc aacagaactt accccggccg tccggacggc aatctgacgg agcagctcac
      601 cttcgccatc atggagctga accggaaaga gaagatcacc ctcttcgtcg acgcgcacga
      661 ggcttccctc atgtaccccg tggtctccac ctacgtggcc cacgaccggg cgctcgacat
      721 ggcgatgatg gcgtccatga tgctgtcggc caccgagttc ccgatgaagt gcgaggcctc
      781 ccccaagaac ctgcgggggt tgagccaccg cgacgcgggc gatttctgcg actcgctggt
      841 catcctgatg gagacgcccg aaccgttcat cgacagagtg gcgggcaaaa tcaccgaaaa
      901 gctgatgatg gacggaaagg acgagttcct ccagaccgcg gcggagcacg gcctgctgtt
      961 caccgactac gacatctccg tcggctgccc catgtgggac agggtagggc gccatctctc
     1021 cggagcgttg gcaatgatcc agcagatgaa cgacttcctg atggagccgg agaaggagtt
     1081 gcagctctcc tttccgaact actccgactt gaaggaacac ggactcggcc atttcctgaa
     1141 cgatcctgcg aaagcggatc cggcgaaggt ctttttgaac tgacggcact atcccgtaag
     1201 gcttctcccg gcgggagcgt tcccgccggt gcaccgcgcg cccgaaggct ttggtatgca
     1261 tcatttattt ttatttcaga caggaggaaa aacacgtgtt gaaatcattc agaaccatgc
     1321 tgtgcgccat gctgtgcgtc atccttctct cctcggccgc ccttgcctgc accgttcttg
     1381 cggtgggtaa agacgccacc gtggacggaa gcgccatgat cacgcacaac gacgactcca
     1441 gggtcgccaa cagccgcctc ttcatcattc ccgaggccga ctggccggaa ggttcgatgc
     1501 ggagcgtcgt gaaggacgcc cacggctacg agggcgacgg acaggtcatc ggcgagatcc
     1561 cgcaggtccc gcacaccttc cggtacttct tctcccgcta ctccttcatg aacgagaagg
     1621 gcgtcgccat cagcgaatcg accgactccg tcgaggtcac cgacgagcgg agcaagaagg
     1681 tcgaacagat catggcgaag gacgccgtgg gaatgatgga cgcctggatc cttcaggaca
     1741 tcatgctcga gcgcgcgagc acggcccgtg aagcggtcga gatcatgggc aaactcgtcg
     1801 aggagtacgg ctggtacgac gctcccgaga cgatgcccct gaccgacggc aacgaagtct
     1861 ggatcgccga gttctacggc aacaagatct gggccgcatg gcgcatgccc gacgaccaca
     1921 tcttcgtggg cgccaaccgc gcgcgtctcc gccacctcga cctgaccgac aaggaaaacg
     1981 tcatgtactg ccccgacctc gtcgagttcg ccgtgaagaa cggattcatc gaggaaaagg
     2041 acgtcaatat gaaggacttc agcccggccg acgtctactg cccgagcacc gcgctctacg
     2101 cgacacgccg cgagtggaga gtcctctcgc tcgccgctcc cgactccttc aagctcggcc
     2161 ccgacgaata cgactacccc atgtccatca aaccggacaa gaagctcagc gtgcatgacg
     2221 tgctgcgcat caagggcgac tggtacgagg gcactccctt cgatctgagc aagggcatcc
     2281 aggcaggccc ctggggcaac cccatccgct tcgccaattc cagcaagaag aatccc
//
LOCUS       DAOPKU010000484         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_53158_length_2334_cov_4.603335, whole genome shotgun sequence.
ACCESSION   DAOPKU010000484 DAOPKU010000000
VERSION     DAOPKU010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53158_length_2334_cov_4.603335"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PK849_12560"
     CDS             <1..655
                     /locus_tag="PK849_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPE67005.1"
                     /translation="YVPGAGRGAGGAAKATLLFCHGNAGNISHRIDSLAIFHRLGVST
                     FIFDYRGYGESEGTPSEQGVYSDVEGAWSWLTKERGVPPEQIILFGRSLGGAVAAYAA
                     SRGEKFSPRGLILESTFTSLASVGQSHYFFLPMKLIVGNAFDTLSRLERIDCPVLVAS
                     SPEDEVVPGRHGRILFEAAREPKWFLPLKGDHNLGYMTTGRAYVDGLDAFFRNVLGE"
     gene            684..1841
                     /locus_tag="PK849_12565"
     CDS             684..1841
                     /locus_tag="PK849_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HPE67006.1"
                     /translation="MEFFESLLKNHLFLMSLTVALGILVGKISVRGINLGISGALFVG
                     LFIGAQGYSVPKEYFAWNLMFFVVAVGLLAAEDTARVVKRYGLKFILLSVAVTGAGAL
                     ATLAAGILFSGSASPNMIAGTYTGALTSSPGLGAALEATGGSPDVTIGYSVAYPFGVM
                     AVVLFVQLIPAIFGIDVAKEREKLALERKAAEPASPDARPASIPFSLLSFVICIIGGS
                     VLGGISLPLGPLGSVGLGTTGGALLFALGVGSFDHIGPLQMRMEKNVLSAVRAISLGF
                     FLAVVGLNAGGGVVKAFLEHGVLLIAVGIGSALAAEMVGFFLGHYVWKINWIILAGAI
                     CGAMTSTPGLGAAIDATGTDECSAGYGGAYPVAIVCMVLFTTMLAAFFKAR"
     gene            1892..>2334
                     /locus_tag="PK849_12570"
     CDS             1892..>2334
                     /locus_tag="PK849_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HPE67007.1"
                     /translation="MFTTIRRWAEAHPFFFAPGFVGLATLLFLPGRELLGKGHWALLY
                     LLVISVVASVVGTRPSFVAAVTSFLTWNVFFLPPYNTFRVDDPKDWISLGVFLIVGLL
                     IGLTSGRLRERETTALSQEREMALLNRLTGRLLAIEGALEMAETL"
BASE COUNT          356 a          692 c          785 g          501 t
ORIGIN      
        1 gtacgtgccg ggcgcagggc ggggggcggg cggagcggcg aaggcgacgc tgctcttctg
       61 ccacggcaac gcgggaaata tctcgcaccg gatcgattcg ctggcgatct ttcaccggct
      121 cggcgtctcc accttcatct tcgactacag ggggtacgga gagagcgagg gaacgccctc
      181 cgaacagggg gtttacagcg acgttgaagg tgcgtggagc tggctgacga aggagcgcgg
      241 cgttcctccc gagcagatca ttctcttcgg caggtcgctc ggcggcgcgg tggcggcgta
      301 cgcggcatct cgcggcgaaa agttctcccc gcgagggctg attctggagt ctacctttac
      361 ctcgcttgcc tcggtcgggc agagtcacta cttctttctt ccgatgaagc tgatcgtggg
      421 gaacgcgttc gacaccctct cccgactcga gcggatagac tgcccggttc ttgtggcgtc
      481 gagcccggag gacgaagtgg tccccggcag acacggcaga attctcttcg aggcggcgcg
      541 cgagccgaag tggtttctcc ctctgaaagg agaccacaac ctcggatata tgacgacggg
      601 gcgggcgtac gtcgacggat tggacgcttt cttccggaac gtcctcggcg agtaagttca
      661 caacagataa ggagatgttt ccagtggagt ttttcgagtc ccttctcaag aatcacctct
      721 tcctgatgag cctgacggtc gcattgggca ttctcgtcgg gaagatcagc gtcagaggca
      781 tcaacctcgg gatatcggga gcgctcttcg tcggcctgtt catcggcgcg caggggtact
      841 ccgttccgaa agagtacttc gcgtggaatc tgatgttctt cgtggttgcg gtgggcttgc
      901 tggccgccga ggataccgcg cgggtcgtca agcgctacgg gctcaagttc atcctgctct
      961 ccgtcgccgt cacgggcgcg ggggcgctcg ccacgctcgc cgccggaatt ctcttctccg
     1021 gctcggcgtc gccgaacatg atcgcgggga cgtacaccgg cgcgctcacc agttcgccgg
     1081 ggctcggtgc tgctctcgaa gccaccgggg gaagcccgga cgtcaccatc ggctactcgg
     1141 tcgcgtaccc cttcggcgtc atggcggtgg tgctcttcgt gcagctcatt ccggcgatct
     1201 tcggcatcga cgtggcgaaa gagcgggaaa agctcgcgct tgagcgaaag gcggcggagc
     1261 cggcatctcc ggacgcgcgt ccagcttcga tcccgttttc gctgctctcc ttcgtgatct
     1321 gcatcatcgg cggctccgta ttgggcggga tttcgcttcc gttggggccg ctcggctccg
     1381 tcgggcttgg gacgacgggc ggtgcgctgc tcttcgccct cggcgtcggc agcttcgatc
     1441 atatcggccc gctgcagatg cggatggaga aaaacgtcct ctcggcggtg cgcgccatct
     1501 cgctcggctt cttcctcgcg gtggtggggc tgaacgctgg gggcggcgtg gtgaaggcct
     1561 ttcttgagca cggggttctg ctcatcgccg tcggtatcgg ctcggcgctg gcggcggaga
     1621 tggttggttt cttccttggg cactacgtct ggaagatcaa ctggatcatc ctcgccgggg
     1681 cgatctgcgg cgcgatgacg agcacgcccg gcctgggcgc ggccatcgac gcgacgggga
     1741 cggatgagtg cagcgcgggg tacggcggag cctatcccgt ggccatcgtc tgcatggtgc
     1801 ttttcaccac gatgctcgcg gccttcttca aggcgcggta gaggaagcaa tcggtttccc
     1861 gccgcttcaa gcggtcgata aggagaaaga tatgttcacg acgatccgga gatgggccga
     1921 ggcgcatccg ttctttttcg ccccgggatt tgtggggctt gcaacgctgc tgttcctgcc
     1981 ggggagagag cttctcggca aggggcactg ggcgcttttg tatcttctgg tcatttctgt
     2041 agtggcctcc gtcgtcggga cgaggccgtc cttcgtggcg gcagtgacgt cgtttcttac
     2101 atggaacgtc ttttttctcc ctccgtacaa cacgttccgg gtggacgatc cgaaagactg
     2161 gatttctctc ggggtttttc tgatcgtcgg tctcctgatc ggcctgacct cgggacgcct
     2221 tcgggagcgc gaaacgacgg cgctctccca ggagcgggag atggcgctcc tcaacaggct
     2281 gaccgggaga ctgctcgcca tcgaaggcgc ccttgagatg gcggagacgc tctg
//
LOCUS       DAOPKU010000485         2332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_53224_length_2332_cov_5.166447, whole genome shotgun sequence.
ACCESSION   DAOPKU010000485 DAOPKU010000000
VERSION     DAOPKU010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53224_length_2332_cov_5.166447"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PK849_12575"
     CDS             complement(<1..316)
                     /locus_tag="PK849_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67008.1"
                     /translation="MTHPLSDTLRHKKLYLLDMDGTLYLGDRLFPVALPFLNALRAAG
                     ARYLFLTNNSSRGTGSYVQKLGGMGISACEEDFFTSTDAACAYLRAHYRGNKIYALGT
                     ASF"
     gene            543..1721
                     /locus_tag="PK849_12580"
     CDS             543..1721
                     /locus_tag="PK849_12580"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPE67009.1"
                     /translation="MLDQLIAVLDLATLEIALGATSLISCATMLALWRTNSSEKGPAC
                     WAAATFLGGSGLFAHFFLLNFIGGAETLRVTLPFVVGSFPLALLLALEGTLRFQGIGG
                     ERKRAYVLPVFAVALLCTSFLGAENASARYMINDPIMILILLLIAFFLLKRTEGLERG
                     IHAIPSFCCILLAATLGYRCFFAFSGATGSEIHSSSRLVYFAFLLWAPGWTCGLCMAV
                     MFRARRFILKIADHDSLTGLANRRKFDRTADLLLGRNNSIRKKFALLLIDLNGLKQLN
                     DLHGHATGDRALVLMSRALEHTVGPDDIAARFGGDEFVVLTREVKDRDDLERLEQTIR
                     DSVEREHDLDEERKLLLRMSIGSALFPEDGRNTGELLYIADQRMYLEKQTRRSLSKVV
                     "
     gene            complement(1788..>2332)
                     /locus_tag="PK849_12585"
     CDS             complement(1788..>2332)
                     /locus_tag="PK849_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE67010.1"
                     /translation="CAGLLTPDRGDALLAGAPVSAMRAKERAARVGFLFQDAERQIFH
                     STVAEEVLFSLRDEPLSSGERMERLDAALCATGLRGKEKKHPLDLNAAERRMVAVASI
                     AVRGAELLLLDEPSRDFDPQWQELFEEWLRGHNAAVLAISHDPDFVERLFPQVWELNQ
                     GRLSQRRGREAPFSIDQRNS"
BASE COUNT          471 a          714 c          691 g          456 t
ORIGIN      
        1 ggaaggatgc ggtacccaga gcgtagattt tgttgcccct gtagtgggcg cgcaggtagg
       61 cgcaggcggc atccgtggag gtgaaaaaat cctcctcgca agcggaaatg cccatgccgc
      121 ctaacttttg cacatagctg ccggtacccc ggctggagtt gttggttaaa aagagatacc
      181 gggcgcccgc cgcgcgcagg gcattgagaa agggcagggc gacagggaac agccggtcgc
      241 ccagatacag ggtgccgtcc atgtccaaaa ggtacaactt tttatggcgc agggtgtcgg
      301 aaagaggatg tgtcataatg tctcctttgt ttgcttgttt gtcacgagcg ttgaaataaa
      361 ctcataatag cccgaaatac aatggctggc aaggaaaaga aaggaaagtt tatcgcaggc
      421 gacaaaatag tacaaaaaac ccgcgcgaaa tcacatcaaa agtgatagaa ttgcgacgca
      481 ttattttttc cacgacggcg cgcaacggac attcagacaa atatttgcgg aggcgaagac
      541 caatgctcga tcaactcatc gccgttcttg acctggcgac gctcgaaatc gccctcgggg
      601 cgacctccct gatctcatgc gccacaatgc tcgcactctg gagaacgaat tcctccgaaa
      661 aaggccccgc ctgctgggcg gcggccactt tcctcggagg ttccggtctg tttgcgcatt
      721 ttttccttct gaatttcata ggaggggccg aaacccttcg cgttacgctc cctttcgtcg
      781 tcggcagctt tcccctcgca ctgcttctgg cccttgaggg aactctccgt tttcaaggta
      841 tcggcggcga acgcaaacgg gcgtatgtac tgcctgtgtt cgcagtcgca ctgttatgta
      901 cgtcttttct gggcgcggag aacgcctctg cacgatacat gatcaacgac cctatcatga
      961 tcctcatcct gcttctcatc gcgttcttcc tgctgaagag aactgaagga ctggaacggg
     1021 gcatccacgc aatcccttcg ttctgctgca ttctcctcgc ggcgacgctg ggctaccgat
     1081 gtttcttcgc cttctccggg gcgaccggaa gcgaaattca ctcatcctcc cgactcgtct
     1141 acttcgcgtt cctcctctgg gcgccggggt ggacctgcgg cctctgcatg gcggtgatgt
     1201 tcagggcgcg gcgctttatc ctgaagatcg ccgaccacga cagcctgacc ggcctcgcca
     1261 accggagaaa gttcgaccgg acggccgacc tgctgctcgg gcggaataac tcgatacgaa
     1321 agaagttcgc cctcctgctc atcgacctca acggcttgaa gcagctcaac gatcttcacg
     1381 gacacgccac gggagacagg gcgctggtgc tgatgagccg cgcgcttgaa cataccgtcg
     1441 gccccgacga tatcgccgcc cggttcggcg gagacgagtt cgtggtgctg acgcgcgagg
     1501 tgaaggaccg cgatgacctg gagcgtttgg aacagacgat ccgtgactcg gtggagaggg
     1561 agcacgatct cgacgaggaa cggaagctcc ttctgaggat gagcataggg agcgcgctct
     1621 ttccggagga cggccgcaac accggggagc tgctgtatat cgcggaccag aggatgtacc
     1681 tggaaaaaca gacccgccgg tcgctctcca aggtggtgta ggcttccgaa tcagtgcgcc
     1741 gccccgggat cgccctgtgc gctcggggcg gcggcgaccg ggggctttca gctgttcctt
     1801 tgatcgatag agaagggcgc ctcacgaccg cggcgttggc tgagccgacc ttggttcaac
     1861 tcccagacct gcgggaagag acgctcgacg aagtccggat cgtgactgat cgcgaggacc
     1921 gccgcgttgt gtccgcgaag ccactcctcg aacagctcct gccactgcgg atcgaagtcg
     1981 cggctcggct cgtcgagcag gaggagctcc gcgccccgta ccgcgatcga agcgacggcg
     2041 accatgcgcc gctccgccgc gttcaagtcc agcggatgct tcttctcctt cccccgaagc
     2101 cccgtcgcgc agagcgccgc gtccagccgc tccatccgtt cgccggagga gagcggttcg
     2161 tccctgagcg agaagaggac ctcctcggcc accgtcgagt gaaaaatctg ccgctccgcg
     2221 tcctgaaaga gaaagcctac ccgcgccgcg cgctctttcg cccgcatcgc cgagacgggc
     2281 gctccggcaa gcagcgcatc gccccgatcc ggcgtcagca atcctgcgca ga
//
LOCUS       DAOPKU010000486         2332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_53238_length_2332_cov_2.985507, whole genome shotgun sequence.
ACCESSION   DAOPKU010000486 DAOPKU010000000
VERSION     DAOPKU010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53238_length_2332_cov_2.985507"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            615..1067
                     /locus_tag="PK849_12590"
     CDS             615..1067
                     /locus_tag="PK849_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67011.1"
                     /translation="MLDIGDYRENVALMLDISILGPLPCTLFFLFAMPVAEMSMLFQN
                     TLYMKDVGYSFVASFLRERFSPFLVTTDHLPLLLAQYFLLAGRFRPAFGLIAFANLQI
                     SLRQSILHTLETEGNEKDGSAVRAVNGLLNLLFLLSLTAFLLKALGAA"
     gene            1111..1479
                     /locus_tag="PK849_12595"
     CDS             1111..1479
                     /locus_tag="PK849_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67012.1"
                     /translation="MIERDFGMEDVLSFLTGILMRENLSDLVEIASFVLERKVSLKEL
                     PFIADEIRRRILKQHPWLRSLEEEDGECVEIAEWVRSVKAKYGETILLKAEDFSKKNR
                     FRRLRERAAKLLFKTERLGF"
     gene            1744..>2332
                     /locus_tag="PK849_12600"
     CDS             1744..>2332
                     /locus_tag="PK849_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67013.1"
                     /translation="MREINTGCTAEEENEPETYMLREGVGLFVDYFRNRFPDLEEYRV
                     RYLPVIEMLAASPTPLPERAVGEILHRGRTETRALLNELGPLFPISPISSAFFGNEGE
                     PETFVSISSEYGSALRSWLKTPAAGGYAADERRGHQRLAERGYLRYRKHGPEGLAGAM
                     LLQLPVHLIESGMAGSCVAYLADPRVRERAAELGVS"
BASE COUNT          513 a          628 c          661 g          530 t
ORIGIN      
        1 gctcaagagc ttgagcgcct tcaatacaca gtttctctct agtaaaaaat ggaggctttt
       61 aatttcgtct atcgatttct ctacattttt atcccttcca gtggcaaaat caaagccaga
      121 tgcgagtaat cgttctattt tcatcctttg ctcgctttct tatcaaaaga agagctcgat
      181 ctttttccgg ttttgacgaa aaacagtgcg gaaaaatttg acagaccttc cgaagttgaa
      241 tataatctcc gtccttgttc acttcacctg attcgacgac accggtaaaa aaacatccat
      301 tttaagtttt tcggcgaaca cagtcttctc cgttccagga gaaaaagaga ttcaaccatc
      361 gcttcagatc gcaaatccat cgtttcgctt caagagcccg tcaggagtac gacgtaacat
      421 gtcgctttca tgctgtacga ttttcgtccg gtaaataact ttctatcgga tgcggcgcac
      481 attgacaatc ggaacgggag atatccgacg gtcccggagg ttttcaccga aagcggggct
      541 aaattatttg gtttcctcga ttccgaagac cggagaatat ctttccgaga tcacacgcag
      601 gagggaaaag acgaatgctc gatatcggtg attacagaga gaacgtcgcg ctgatgctgg
      661 atatttcgat cctcggtccg cttccctgca cgctgttctt cctctttgcg atgccggtgg
      721 cggagatgag catgctcttc cagaacacgc tctacatgaa ggacgtaggg tactcgttcg
      781 tggcctcgtt tctgcgggag cggttcagcc cgtttctggt gacgactgac catctgccgc
      841 ttctcctcgc ccagtacttt ctcctggcgg ggcgcttccg gccggctttc gggctgatag
      901 ccttcgccaa cttacagatc tcgctccgcc aaagcatcct ccacacgctg gaaacggagg
      961 ggaacgaaaa agacgggagc gcggtccgag cggtcaacgg tcttttgaac ctgctctttc
     1021 tgctctctct gacggccttt cttctgaagg ccctcggagc ggcgtagaca agataatccg
     1081 cgaacaggcg aaaggaaagg acgggagaag atgatcgaac gggatttcgg catggaggat
     1141 gtgctcagct tcctgacggg cattctgatg agggagaatc tctcggatct cgtggagata
     1201 gcctccttcg ttctggagcg gaaggtctct ctcaaagagc ttccattcat cgccgacgag
     1261 atacggaggc gcattctgaa gcagcatccg tggctgcgct ctctcgaaga ggaggatggg
     1321 gagtgcgtgg agatagccga atgggtgcga tctgtcaagg cgaaatacgg cgagacgatc
     1381 ctcttgaagg ccgaggattt ctcgaagaag aaccggttcc ggaggctgcg ggagagagcg
     1441 gcgaaacttc tcttcaagac ggagaggctg ggtttttagg ggatcaggca tccacggagg
     1501 agagggtgcg gcagacatcg cgcgaaacgc tctcgaagcc cggcgtgaag gaaggcgatg
     1561 ttttacagga tggggcgctg cgctcccctt cgtcttacgt tgtcgctctc ctgcagcagc
     1621 atcgcctcca tatcttcttt tcccccgttt tcaaatagag atggggagga gtttcattgg
     1681 gaaagaacca ttcggagcgc ggcgaaaccg gaccgcgaag gaggagaaga aaggagcgca
     1741 cggatgcgcg aaataaatac aggatgcaca gcggaggagg agaacgagcc ggagacgtac
     1801 atgctccggg agggcgtcgg tctgttcgtc gactactttc gaaaccgctt ccccgatctt
     1861 gaggagtacc gcgtgcgata ccttcccgtg atcgaaatgc tcgccgcatc acccacgccc
     1921 cttccggagc gcgctgtcgg ggagattctc caccggggac gcacggagac gcgtgctctt
     1981 ctgaacgaac tcgggccgct cttccccatc tcgccgatct cctccgcttt cttcggaaac
     2041 gagggggagc cggaaacgtt cgtctcgatt tcaagcgagt acgggagcgc gctccgctcc
     2101 tggctgaaaa cccctgctgc cggcgggtac gccgcggacg aacgacgcgg tcaccaaagg
     2161 ctcgccgagc gaggatatct tcggtaccgg aagcacggcc ccgagggcct tgcgggggca
     2221 atgctcctcc agcttcccgt tcatcttatc gagagcggaa tggcgggttc ctgcgttgcc
     2281 tacctcgccg atccccgtgt tcgtgagagg gctgccgaac tcggcgtatc tc
//
LOCUS       DAOPKU010000487         2325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_53464_length_2325_cov_5.143612, whole genome shotgun sequence.
ACCESSION   DAOPKU010000487 DAOPKU010000000
VERSION     DAOPKU010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53464_length_2325_cov_5.143612"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2308)
                     /locus_tag="PK849_12605"
     CDS             complement(<1..2308)
                     /locus_tag="PK849_12605"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HPE67014.1"
                     /translation="MKQSVFHRCAKRTALCAGLVVLLCVFAPAGFSAGEAAPPKIPLK
                     EAALKDFSSVPSPMKLDVVPNFKETAFPLDKAANLKEVEEVVGKLTAEQKSALQKNRF
                     LLLQKPSLHTFSSMGRYDEMLCNFEGIGGSYDPAYREPWNTRFVGPDVFLHALHTFFS
                     KRLEAMEGGELLGAVQYMLEELYANAVSLRSLASKSNKPHWERLQAQLVVPLILALNC
                     DESVEPVWTDPDAEPAQQTDSLKNALALFKKYGKSFSKKTADAVAEELQRIYAAEEAL
                     KGLLGLSPAYASQSVDYTQFTPRGHYEKNSRSRAYFRTMIWLGQLGWKLADEEGAVDA
                     LNFALAMSYKRPVARGKPQRPALGALTAPLDAWKRVMEVSCFFVGYPDGASYPEWRQL
                     LLKKAGVSAFTPDTCGDAAAVDRIREASEELAPSIPHFNKLFSPASTETFCVFPQRFT
                     IPWLITDELTYKVNVREDLPVLFSSLWVAAVTGNRYALDLLPKQIPLNLAALPAATAE
                     GAPAPEKRVDIPEDHLRAVVAAMPGAISSLREKLSAEPESAWFSSIGTAWMHLLGTLA
                     SEFGKGYPLYMQSPLFSAKQLETQLGSYTELRHDTILYEKPNYAEMGDGGDELPPKPL
                     PKGFVEPNLPFWNELLRVVDYIQNGFEKNVLFPMDMEEYGALTQFRQTVQRCGKIAAK
                     QLQGKALTEEEYEFIRLFSLDYMAAPADGYGAVPPDELFQSGLIVDIQTMNLDPIAIG
                     DPAILYQATAEPSIMLVLVGNENTPRITI"
BASE COUNT          406 a          683 c          767 g          469 t
ORIGIN      
        1 cgatcgtgat tcgaggcgta ttttcgttgc cgacgagcac cagcatgatc gaaggctcgg
       61 cggtcgcctg gtagaggatg gccgggtcgc cgatagcgat cgggtcgagg ttcatggtct
      121 ggatatcgac gatgagtccg gactggaaga gctcgtccgg aggcaccgcg ccgtagccgt
      181 ccgccggggc ggccatgtag tcgagcgaaa acagacggat gaactcatac tcttcttcgg
      241 tgagcgcctt gccctgaagc tgcttcgctg cgatcttgcc gcatcgctgc accgtctgcc
      301 ggaactgggt gagcgcgccg tactcctcca tatccatcgg gaagaggacg tttttctcaa
      361 aaccgttttg gatatagtcg acgacgcgga gcagctcgtt ccagaaggga aggttcggct
      421 cgacgaaacc cttgggaagc ggctttggag gaagttcgtc gcctccgtcg cccatttcgg
      481 cgtagttcgg cttctcgtag agtatggtgt cgtgccgcag ctcggtgtac gagccaagct
      541 gcgtctcaag ctgtttggcg gagaataagg gactctgcat gtacagcggg tacccctttc
      601 cgaactcgga ggcgagcgtt cccagcaggt gcatccaggc cgttcctatg gagctgaacc
      661 atgcggactc aggctctgcc gaaagttttt cgcgcaggga tgagatcgcg cccggcatcg
      721 ccgcgacgac ggcgcgcagg tggtcttcgg ggatgtcgac ccgtttttcg ggggcgggcg
      781 cgccttccgc cgtggccgcg ggaagcgcgg cgagattcag cgggatctgc ttcgggagga
      841 ggtccaatgc gtaccggttc ccggtgacgg cggccaccca cagactggag aagagtaccg
      901 gcagatcctc gcgcacgttc accttgtagg tcagctcgtc ggtgatgagc cagggaatgg
      961 tgaagcgttg cgggaagacg cagaatgtct cggtactcgc cggtgagaag agcttgttga
     1021 agtggggtat ggaaggagca agctcttcgc tcgcctccct gattcggtcg acggcggccg
     1081 cgtcgccgca ggtgtccggc gtgaaggcgg agacgcccgc cttcttcagc agaagctgcc
     1141 gccactccgg gtagctcgcg ccgtctgggt agccgacgaa gaagcaggag acttccatca
     1201 cgcgtttcca cgcgtcgagg ggagctgtca gcgcgccgag agccgggcgc tggggcttgc
     1261 ctcgggcaac cggccgttta tacgacatgg cgagggcgaa gttgagcgcg tcgacggcgc
     1321 cctcttcgtc ggcgagtttc cagccgagct gtcccagcca gatcattgtc cggaagtagg
     1381 cgcggctgcg cgagttcttc tcgtagtgcc ctctgggggt gaactgggtg tagtccacgc
     1441 tctggctcgc ataggccgga ctgaggccaa gaagcccttt cagcgcctcc tctgcggcgt
     1501 agatccgttg cagctcctcg gcgacggcgt cggccgtctt cttcgagaag gatttgccgt
     1561 actttttgaa gagcgcgagc gcgtttttca gactgtcggt ctgctgcgcg ggttcggcgt
     1621 ccggatcggt ccagaccggt tcgacgcttt cgtcgcagtt gagggcaagg atgaggggga
     1681 cgacgagctg cgcctgaagt cgctcccagt gcggcttatt gctcttggac gcgagcgaac
     1741 ggagcgagac ggcgttggcg tacagctcct cgagcatgta ctgtaccgcg cccaggagct
     1801 cgcccccctc catcgcctca aggcgctttg agaagaaggt gtgaagcgca tgaagaaaga
     1861 cgtccgggcc gacgaagcgg gtgttccacg gctcgcggta ggcgggatcg tagcttcccc
     1921 cgatgccttc gaagttgcac agcatttcgt cgtaccgccc catcgacgaa aaagtatgca
     1981 gactgggttt ctgcagcagc aaaaagcggt tcttctgcag cgcgctcttc tgctcggccg
     2041 tgagcttgcc gacgacttcc tccacctctt tgaggttcgc cgccttgtcg agcgggaacg
     2101 ccgtctcttt gaagttcggg acaacgtcga gcttcatcgg ggacgggacg ctggaaaaat
     2161 ccttcagcgc cgcctccttc agcggaatct tcggcggcgc ggcctctcct gccgagaaac
     2221 cggccggcgc gaaaacgcaa agaagcacaa ccagacctgc gcacagcgcc gtccgcttcg
     2281 cgcatcggtg gaacacgctc tgtttcatgc cacattcctc ctttt
//
LOCUS       DAOPKU010000488         2324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_53504_length_2324_cov_3.955046, whole genome shotgun sequence.
ACCESSION   DAOPKU010000488 DAOPKU010000000
VERSION     DAOPKU010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53504_length_2324_cov_3.955046"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PK849_12610"
     CDS             complement(<1..964)
                     /locus_tag="PK849_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPE67015.1"
                     /translation="MQFPKNEPVTDRPLVVLLPAAMEQSPRTDVADALRGREFVSGIL
                     RAHGYVPAPLDITPEILQFPERIQHIVRTTKTRCVFNIFEGFGCDSASEHQFRSLVEE
                     MSIPCTGNPSGVLQACLSKETCSRTLRKAGIPVPEGVSLHPGFDPELLASLRLPVFVK
                     PLMEDGSVGIDASSFVQEKSALLRTVLEKLSLFPRGVRAEEFIPGREYSVACIGNGPY
                     NVLGVSVIDYEQTEGTNFLDYDSKWDPASPLYSLVPQRAEGAFREKAAQLAAAAGRAL
                     GCRGYFRVDMRERHGILYVLDVNPNPDITPDGGFLRQCREAGFSD"
     gene            complement(969..2033)
                     /locus_tag="PK849_12615"
     CDS             complement(969..2033)
                     /locus_tag="PK849_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPE67016.1"
                     /translation="MNEEYGHEPDVPLQPVARVSVVYNLKKQAGSDEPDDKYEEYDPI
                     ATVEAVADAVGAYGFSVSLCEQDDDFAARLAEQKPQFVANIAEGRGEARGREAQVPCF
                     LESAGIPYWGSDAVSMAVALDKLLTSRTLLCDEIPVPLCACFKQEQDLSQLPALFTER
                     SRFVVKPRYEGSSKGIFSDSVAHTPDEAKEKIRRIWGRYAQPALLEEYLPGDEITVGI
                     VGNGRPSVVGMMRIVPVEQREEFLYSLEEKRDYLKRIRYEGPESIPASVRDQVGRFAL
                     KAFQSLELRDMARVDFRLDSDGTPRIIDINPLPGLSPEYSDLPILHRLSGGEYNDLVR
                     SILRSSFSRNGLAFPARGSR"
BASE COUNT          465 a          674 c          706 g          479 t
ORIGIN      
        1 cgtcggaaaa ccccgcctca cggcactggc gcaaaaaccc tccgtccggc gtgatatcgg
       61 ggttcggatt tacgtcgagc acgtagagaa tcccgtgccg ttcacgcatg tccacccgga
      121 aatagcctct gcagccaagg gctcggccag ccgccgccgc gagctgcgcg gctttctccc
      181 ggaacgcgcc ctccgcccgc tgcgggacaa gcgagtagag aggcgaggcg gggtcccatt
      241 tagaatcgta atcgagaaag ttcgttcctt cggtctgttc gtagtcaatg accgagacgc
      301 caagcacatt gtacgggccg ttcccgatgc aggcgacgga gtactcgcgt ccgggaatga
      361 actcctccgc ccggactccc cgaggaaata gtgagagttt ctccaggacc gttcgaagca
      421 gcgcgctctt ttcctgcacg aacgaggagg cgtcgatgcc tacgcttccg tcctccatca
      481 ggggtttgac aaacaccggc aagcgaagcg atgccaagag ttcaggatcg aatcccgggt
      541 ggagcgagac cccttcgggg acaggaattc ccgccttcct gagcgtgcgc gagcacgttt
      601 ctttcgagag gcaggcttga agcacccccg aaggattgcc ggtgcagggg atggacatct
      661 cctcgacgag cgaccggaac tggtgttcgg aggcgctgtc gcagccgaat ccttcgaaga
      721 tgttgaagac gcatcgtgtc ttcgttgtgc ggacaatatg ttggatgcgc tccggaaatt
      781 ggagaatctc gggcgtgata tcgagaggcg ccgggacgta tccatgagcc cgaagaatgc
      841 cggagacgaa ttcacgcccc cggagcgcat cggcgacgtc cgtgcgcgga gactgttcca
      901 tcgcggccgg aagcaggacg acgagaggcc tgtcggtcac aggctcgttt ttcgggaact
      961 gcattcgatc accggcttcc cctggcgggg aaggccaggc cgttcctgga gaaggagctt
     1021 ctcagaatgg aacggacaag atcgttgtac tccccgccgc tgagacggtg gaggatcgga
     1081 aggtcgctgt attccgggga gagccccgga agcggattta tgtcgatgat tcttggagtt
     1141 ccgtcgctgt ccagccggaa gtccacccgc gccatgtccc gcagttccag cgattgaaac
     1201 gccttcagcg cgaagcggcc gacctggtcg cggacggaag cggggatgct ctcggggcct
     1261 tcgtagcgga ttcgcttcaa gtagtcgcgt ttctcttcga gagagtagag gaactcttcc
     1321 cgctgttcga ctggaacgat gcgcatcatc cccaccacgg aggggcggcc gttgccgaca
     1381 atcccgaccg tgatctcatc tcccggaaga tattcttcca gcagcgccgg ctgcgcatac
     1441 cgcccccata ttctgcggat cttctctttt gcctcgtcgg gcgtatgcgc gacggaatcc
     1501 gagaagatcc ccttggaaga cccttcgtac ctcggtttca cgacgaaacg ggaacgctcg
     1561 gtgaagagcg cggggagttg cgataggtcc tgttcctgtt tgaaacaggc gcacagaggg
     1621 acggggatct cgtcgcagag aagcgtcctg ctggtgagca gcttgtcgag cgccaccgcc
     1681 atggaaacgg cgtccgatcc ccaatacggg atgcccgcgc tctccagaaa acagggaacc
     1741 tgcgcctccc gcccccttgc ctccccacgt ccttcggcga tattggcgac gaactgaggt
     1801 ttctgttcag caagacgcgc tgcgaaatca tcgtcctgct cgcagaggga gactgagaat
     1861 ccgtatgctc caacagcatc ggcgactgcc tcgacagtcg ctatcgggtc gtactcctcg
     1921 tacttgtcgt ccggctcgtc ggagccggct tgttttttga gattatagac gacggaaact
     1981 ctggcgacag gttgcaacgg aacgtcgggc tcgtgcccgt actcttcgtt catagatttt
     2041 cccctcctga aggatgcatg aatgatgacc tgtgaaaatg cggtcaatgc ggttttccat
     2101 ttcttcgaat ccgatgttcg ggaaccgcca tgtctccctc ctccaagagt ttgccccctc
     2161 cgtgcgaaga gggtctttca gtgcggatta tagaggtttt tgggggaaat acaagaggga
     2221 aattccgttg cgggagagct ttttcgcagg ctgtgcgacg caaaaaaaag gggctccgga
     2281 gagccccttt tctggagttc gcaaagcaag ttttctgtta ggta
//
LOCUS       DAOPKU010000489         2317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_53741_length_2317_cov_4.716622, whole genome shotgun sequence.
ACCESSION   DAOPKU010000489 DAOPKU010000000
VERSION     DAOPKU010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53741_length_2317_cov_4.716622"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PK849_12620"
     CDS             <1..856
                     /locus_tag="PK849_12620"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HPE67017.1"
                     /translation="QTAGLFDEFGKERVVDTPITETAILGLGVGSAAAGLRPIVELMF
                     MDFMGVCMDEINNQAAKMRYMFGGKAKLPMVVRTTCGAGMSAAGQHSQSLEAWLAHIP
                     GVKVVMPATPADAKGLMEGAVRDDNPVFFIEHKGLFGLSGEVPDGEYVVPPGKAAVLR
                     EGKDVSLIAWSAMVHKALAAADMLAKDGISAEVIDLRSIAPLDKKTVLDSVCKTGRVV
                     IVHEACRTGGFGGEIAAIIADEAFDSLNAPVKRVTAPDTPVPFNARLEQLYIPSAEKV
                     VATVKELF"
     gene            896..1924
                     /locus_tag="PK849_12625"
     CDS             896..1924
                     /locus_tag="PK849_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPE67018.1"
                     /translation="MEKDAFVGIIANPASGKDIRRLVAYGTVFDNQEKVNIVRRILLA
                     LDALGVGRVLAMPDYYGIVRRAAEGVRSDRPLRLAVEMLDMPLTGTQIDSQRAAELLC
                     TAGCGCIVTLGGDGTNRMVAKGCRDVPLMPVSTGTNNVFPLLIEGTTAGLAAGVTALG
                     CGEECTVRRSKKLTIFKNGEAADIALIDAVVLQSRFIGSRAMWEVENMRCCAVTRGEP
                     HNIGVASVVGNLSPITPEDPHGGFLKFDPQRADIFAPIAPGLFLPVGVASFCALQPGE
                     EIQVAPVPSIIALDGEREVDLHAGDSASIRLDRDGPRVVDVPAALRWAAESGFFGNKD
                     LVRRLAPL"
     gene            2041..>2317
                     /locus_tag="PK849_12630"
     CDS             2041..>2317
                     /locus_tag="PK849_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPE67019.1"
                     /translation="MAVEIVMPKMGLTMTVGSVGKWLKKEGDQVTKGEHVVEVLTEKI
                     TNLVDAPADGVLRITAPEGTTLPIGGVMGYVGAAGEALPGLPQQEPQS"
BASE COUNT          399 a          701 c          839 g          378 t
ORIGIN      
        1 ccagacggcg ggtctcttcg acgagttcgg caaagagcgc gtggtggata ctcccatcac
       61 ggagacggcg atcctgggcc tgggagtcgg ttccgcggcg gcggggctcc gtcccatcgt
      121 cgagctgatg ttcatggact tcatgggcgt ctgtatggac gagatcaaca accaggcggc
      181 aaagatgcgc tacatgttcg gcggcaaggc caagctcccg atggtggtcc gcaccacgtg
      241 cggcgcgggg atgtccgcgg cggggcagca ctcgcagtcg cttgaggcgt ggctggccca
      301 cattccggga gtcaaggtgg tcatgcccgc gactccggcc gacgccaagg ggctgatgga
      361 gggcgccgtc cgggacgaca acccggtctt cttcatcgag cacaaggggc tcttcggtct
      421 ctcgggcgag gtacccgacg gcgagtacgt cgtgcctccg gggaaggcgg ccgtgctccg
      481 cgaggggaag gacgtctcgc tgattgcgtg gtccgcgatg gtccacaagg cgctcgccgc
      541 cgccgacatg ctggcgaagg acggtatctc cgccgaggtg atcgacctgc ggagcatcgc
      601 gccgctggat aaaaagactg ttctcgactc cgtctgcaag acggggcgcg ttgtcatcgt
      661 ccacgaggcg tgccgcaccg gcggcttcgg cggcgagatc gcggccatca tcgccgacga
      721 ggcgttcgac agtctgaacg ccccggtgaa gagggtgaca gcgcccgaca cgccggtgcc
      781 gttcaacgcg cgtctggagc agctctacat accgagcgcg gagaaagtcg tcgcaacggt
      841 caaggaactc ttctaagcgc tccggagcgg ccggggaaag gggaggggct ccatcatgga
      901 aaaagacgct tttgtcggaa tcatcgccaa tcccgcgtcg ggaaaggaca tccggcgcct
      961 ggtcgcctac ggcacggtct tcgacaatca ggagaaggtg aacatcgtcc ggcggatcct
     1021 gctggcgctc gacgctctcg gcgtggggcg cgtgctcgcc atgccggact attatggaat
     1081 cgtccgcagg gcggcggaag gggtgcgctc cgaccgcccc ctgcgcctcg ccgtggagat
     1141 gctcgatatg ccgctcaccg gcacgcagat tgactcgcag cgcgctgcgg aacttctctg
     1201 cacggccggg tgcggctgca tcgtcacgct tggaggggac gggacgaacc ggatggtggc
     1261 gaaggggtgc cgcgacgtcc ctttgatgcc cgtctccacg gggacgaaca acgtctttcc
     1321 cttgctcatc gaggggacga cggcggggct cgcggccggg gtgacggcgc tcggctgcgg
     1381 cgaggagtgc acggtccggc ggagcaaaaa actcacgatt ttcaagaacg gggaggcggc
     1441 ggacatcgcc ctcatcgacg cggtggtgct gcagagccgt ttcatcggca gccgtgcgat
     1501 gtgggaggtt gaaaacatgc gctgctgcgc ggtgacaagg ggggagccgc acaacatcgg
     1561 cgtcgcctcc gtcgtgggga atctctcccc gatcacgccg gaggacccgc acggcggctt
     1621 cctgaagttc gatccgcaac gcgcggatat cttcgccccc atcgcgccgg gactctttct
     1681 ccccgtcggg gtcgcttcgt tctgcgcgct ccagccgggg gaggagattc aggtcgctcc
     1741 cgttcccagc atcatcgcgc tcgacggcga gcgggaagtg gatctgcacg ccggggattc
     1801 ggcctccatc cggctcgaca gggacggccc ccgcgtggtg gacgtgcccg ccgcgctgcg
     1861 ttgggctgcg gagagcggtt tcttcggcaa caaggacctc gtccggcggc ttgcgccgct
     1921 gtgacgatac ggacacacaa agcccggggt ctcctctttc atgagcggcc tcgggctttt
     1981 tataggcggg cgggagaaag aggcttgggc ccgcaggcag gctgaaagag aggcgagaag
     2041 atggctgtag aaattgtgat gcccaagatg gggctgacga tgaccgtcgg ttccgtcgga
     2101 aagtggctga aaaaggaagg cgatcaggtg acgaagggag agcatgtcgt ggaggtgctc
     2161 acggagaaga tcacaaacct cgtcgacgcg cccgcggacg gcgtgctgcg catcacggcg
     2221 ccggagggga cgacgcttcc catcggcggc gtgatggggt acgtcggcgc ggctggcgag
     2281 gcgctgcccg ggctgccgca gcaggagccg cagagcg
//
LOCUS       DAOPKU010000490         2312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_53906_length_2312_cov_4.101462, whole genome shotgun sequence.
ACCESSION   DAOPKU010000490 DAOPKU010000000
VERSION     DAOPKU010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53906_length_2312_cov_4.101462"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..561)
                     /locus_tag="PK849_12635"
     CDS             complement(28..561)
                     /locus_tag="PK849_12635"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPE67020.1"
                     /translation="MSEVWITTKGGDKGETSIGNGERVPKDHARVETYGTLDECQAHI
                     GMARSLCEYPAIRDYLCLLEKEMSLLMGYIALYPGLTCPEITRLEEITHVVGEVTGKT
                     FRFVRPGDSTPGAALHIARTVARRAERIAVKLFRTGELAEDAYQYVNRLSDVIYALSL
                     WYDHLERERRGEDISCR"
     gene            complement(581..1051)
                     /locus_tag="PK849_12640"
     CDS             complement(581..1051)
                     /locus_tag="PK849_12640"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HPE67021.1"
                     /translation="MNRLIIRHFIPAADSGVRCVPCEETSGYLQSMMESLAPKLVNLD
                     IQLVLQSLEIHEITEANCGKLNYISFFAPELGLEVERPIEEVLNAGVTFERCENCSLA
                     DGTPFAIRTLKTEGSSFAALPPGILSDALIRVVFSTMGSCGKGSCASCAGCGEH"
     gene            1189..1518
                     /locus_tag="PK849_12645"
     CDS             1189..1518
                     /locus_tag="PK849_12645"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPE67022.1"
                     /translation="MTYEDFCQVAEKMIENLPPKLFEQLNGGVNVVRQKKEDGEYVLM
                     GEYVEDPIMGNTVYLYYGTFAEELKGAKRSEWIEEIEETLLHELRHHVESLAGVDYLS
                     FEEMQDL"
     gene            1659..1744
                     /locus_tag="PK849_12650"
     tRNA            1659..1744
                     /locus_tag="PK849_12650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1692..1694,aa:Leu,seq:caa)
     gene            1905..2138
                     /locus_tag="PK849_12655"
     CDS             1905..2138
                     /locus_tag="PK849_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67023.1"
                     /translation="MLGKNEAPNSSNVRWEEALHYFLVFKGAEGRSERTIQDYESHIW
                     RFFKRYPDSFQSGPPLRQAVLSYLTALLSKIGF"
BASE COUNT          611 a          577 c          575 g          549 t
ORIGIN      
        1 ttcaaaaccc cattttttgc atcaggttca gcggcaggaa atatcctccc ctctgcgttc
       61 acgttccaga tggtcgtacc aaagggaaag agcgtatatc acgtccgata atctgttgac
      121 gtattggtat gcatcttcag ccagctcacc ggtgcgaaag agtttcaccg caatacgttc
      181 tgctctcctg gcgacagttc tggcaatatg gagtgcggct ccaggagttg agtcacccgg
      241 ccggacaaaa cgaaaagttt ttcctgtcac ttcgccgaca acatgcgtaa tctcctcaag
      301 cctggtaatt tcagggcacg tcagccccgg atacagggct atatacccca ttagaaggct
      361 catctctttt tcgagaaggc agaggtagtc acgaattgcc gggtattcac acagggaacg
      421 cgccattcct atatgagcct ggcattcatc caaagtcccg taggtttcta cccgggcatg
      481 gtctttgggc actctctcgc cgttaccaat gctggtctct cctttatcgc cgccctttgt
      541 cgtgatccag acttcgctca tcgggataaa ccagactttc tcaatgctct ccgcatccgg
      601 cgcatgaagc gcagctccct tttccgcagc tgcccattgt ggaaaagacc acgcgtatca
      661 gagcatcgct gagtatgccg ggaggaagcg ccgcgaacga actgccctca gtcttgagcg
      721 tccttattgc aaagggagtc ccgtctgcta gagaacagtt ttcacagcgt tcaaaagtaa
      781 ctcctgcgtt cagaacctcc tcgatcgggc gctccacttc aagccccaat tccggtgcga
      841 agaaggaaat atagttcagt ttgccgcaat ttgcttcggt gatctcatgg atctccaggc
      901 tctgaagcac aagctggata tccagattga cgagtttcgg agcgagactc tccatcatcg
      961 actgaagata accggatgtt tcttcgcagg ggacgcagcg aacaccggaa tccgcagcgg
     1021 gtatgaaatg tcgtataatc agtctgttca ttctcgatcg acctccttga tgtgatccat
     1081 gcattacatt atacactcgg agggtgtttt aagggaaaag gtatggacac gattcctctc
     1141 tgaaaacaag gagagcgtga tgaagaaaag aacaaggaga ggtgcgcagt gacgtacgaa
     1201 gatttttgcc aggtagctga aaaaatgatt gaaaacctgc ccccgaagct cttcgagcag
     1261 ctcaacggag gcgtgaatgt cgttcggcag aaaaaagagg acggagagta tgttcttatg
     1321 ggcgagtacg ttgaagaccc tattatgggg aataccgtct acctttacta tggcacattt
     1381 gcagaagaat taaaaggtgc gaaacgcagc gaatggatcg aggaaatcga agagacgctt
     1441 ctccatgaac tgcgccacca cgtggaatct ctggccggtg tagactatct ctctttcgag
     1501 gagatgcagg atctgtagcg acttccccct aaaaaacact ggagggaaac gctatcttaa
     1561 aatgaggaaa aacacttcga ttcttgttgt cgccgcgcaa aattgttctt aactccgttg
     1621 acatgtccgt ttttttcgat taagataccg cagtgcatgc cggggtggcg gaatggtaga
     1681 cgcagcggac tcaaaatccg ccgtgggcga ccacgtgggg gttcgagtcc cccccccggc
     1741 accaatcatt gaaatgactg gcttaaagag ccgatttaaa gtgtaaaagc attgtttttt
     1801 atcccctcaa aaatgacgaa tccgtagtga tgtaaaacga ggggattgcg gcggattttc
     1861 ggggtcttct ttcatcgtcc gggcggcgaa aaggaggccc cgaaatgtta ggaaaaaatg
     1921 aagcgccaaa cagctccaat gttcgttggg aagaagccct gcactatttt ctggttttca
     1981 agggggcgga aggaagaagc gagcgtacca ttcaagatta cgagagccac atctggcgat
     2041 tcttcaaaag gtatccggac tctttccagt ccggaccacc tcttcggcaa gccgttcttt
     2101 catatcttac ggcactcttg tccaaaatag gtttctgaaa aattaaaaag ccccccggct
     2161 ttgagatatc attggtttgc caacacaaaa agatctcaaa acaaaaaaag ggggcaaacc
     2221 aaatgataaa ggcagcgagt ctgttcagtc aaatactttc gaccatccgc accgcagcga
     2281 actttgacaa cctggtggcc aaacacgagg cg
//
LOCUS       DAOPKU010000491         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_54307_length_2301_cov_3.354408, whole genome shotgun sequence.
ACCESSION   DAOPKU010000491 DAOPKU010000000
VERSION     DAOPKU010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54307_length_2301_cov_3.354408"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PK849_12660"
     CDS             <1..1003
                     /locus_tag="PK849_12660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE67024.1"
                     /translation="QANIVASMIFAGISGTAVADTASLGAILIPAMKKEGYDAEFSCA
                     VTAASSTIGPIIPPSMPMIIAGTLTGLSVGRLFLAGAIPGVLLGLGLMVVSWFISKKR
                     NYPKGVKVPRRLLLKEFFGAFWALMLTVLILYGIVGGFFSPTEAAMVACVYAVLVGMF
                     VYKELTLKELPAVILETAITTASIIVLVGLANLFAWILTSEQIPQMVADLLLSITSNK
                     YLIILLINLLLLFVGTFMETIAALLTLFPTLLAVALRVGIDPIHFAMIAVLNLMIGLT
                     TPPVGVCLFVASSIGKVSISRIARAGLPFLAVSIFVLLLVSYIPALSTWLPNLLFNR"
     gene            1074..1829
                     /locus_tag="PK849_12665"
     CDS             1074..1829
                     /locus_tag="PK849_12665"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPE67025.1"
                     /translation="MTSKFSLEGKRALVTGGGSGIGLAISQGIVENGGSVVIVGRREE
                     LLKEACDSMGPKASYMVHDVTDLPGNVGFVKKLEAGGPVDILVNNAGQNIKKPVLETT
                     DEIYHNILEVNLVAVFSLTREISRFMLERKRGAIINIASMASLFGIPYVSAYTSSKAA
                     IAGLTKQLATELSPFGITVNAVAPGFIDTAMSRKAFEGDPERKRKVLSRTPMNELGLP
                     EDVANAVVFLASPAARYLTGVVLPVDGGTSIGF"
     gene            1872..>2301
                     /locus_tag="PK849_12670"
     CDS             1872..>2301
                     /locus_tag="PK849_12670"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF003027.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HPE67026.1"
                     /translation="MILSFRWYGESDPVTLDKIRQVPVLKGIVSALYDVPLGETWPLE
                     KIESLKAAVERKGFVLSAIESIPVHENIKLGRPDRDVLIDKYCASIRNMGKAGIPVLC
                     YNFMPVFDWTRSNMAFPLPDGSNALSYDDVEVLKMDLSKGT"
BASE COUNT          456 a          670 c          674 g          501 t
ORIGIN      
        1 ccaggccaac atcgtggctt cgatgatctt cgccggaatt tcggggaccg ctgttgctga
       61 taccgcaagt cttggcgcca tcctgatccc cgcgatgaaa aaagaggggt acgacgcgga
      121 gttctcctgt gccgtcaccg ccgcatcctc gacgatcggt ccgatcattc cgccgagcat
      181 gccgatgatc attgcgggaa cgctcaccgg cctctccgtc ggccggctct tccttgccgg
      241 agctattccg ggggtattgc tggggcttgg gctgatggtg gtctcctggt ttatatcgaa
      301 gaaacggaac taccccaagg gagtgaaagt gccccgccga ctgctgctga aggagttctt
      361 cggcgccttc tgggcgttga tgctgacagt tcttatcctc tacggaattg tgggcgggtt
      421 cttcagtccc acagaggctg caatggttgc ttgcgtctac gctgttctgg tcggtatgtt
      481 cgtttacaag gaactcacgc tcaaggagct tcctgcggtg attctcgaga ccgcaatcac
      541 gacggcatcg attatcgtgc ttgtcggcct ggcgaacctt tttgcctgga ttctcaccag
      601 cgagcaaatt ccccagatgg tggccgatct attgctgagt atcacgagca acaaatattt
      661 aattattcta ttgattaact tactgctcct ctttgtaggg acgttcatgg agacgattgc
      721 cgcgctgctc acgctcttcc cgacgctcct cgctgtagcg ctgcgtgtgg ggatcgaccc
      781 gattcatttt gcaatgatcg ccgtgctcaa cctgatgatc gggctgacca cgcctccggt
      841 gggagtctgt ctcttcgtcg cttccagcat cgggaaggtc tccatcagcc gtatcgcgcg
      901 ggcaggactg ccatttcttg ctgtgagcat cttcgtcctg ctgctggtct cctacatccc
      961 agcgctttcg acgtggctgc ccaatctgct cttcaaccgc tgaagcacga tgaagcacga
     1021 ccgccgggag aataagcggc gaatgtacga gaaagtgaaa ggagaaatac gacatgacat
     1081 ccaaattttc gcttgaggga aaacgggccc tggtaacggg cggcggaagc ggcatcggtc
     1141 tcgccatctc gcaggggatc gtggagaacg gcggttccgt ggtcatcgta ggccggaggg
     1201 aggaactcct caaggaagct tgcgactcca tggggccgaa agcgtcgtac atggtccacg
     1261 acgtcacgga tcttccgggc aacgtcgggt tcgtcaagaa gctggaagcg ggcgggcctg
     1321 tcgacatcct cgtgaacaac gccggacaga acatcaagaa gcccgtcttg gagaccacgg
     1381 acgagatcta ccataacatc ctcgaggtca acctggtcgc cgtcttctcg ctcacacgcg
     1441 agataagccg cttcatgctc gagcgaaagc gcggcgcgat catcaatatc gcctcgatgg
     1501 cgtcgctctt cggcattccg tacgtgagcg cgtacacgtc ctccaaagcg gctatagcag
     1561 ggctgacgaa gcagcttgct acggagctct cccccttcgg tatcaccgtc aacgccgtgg
     1621 cgcccggttt catcgatacc gcgatgtcgc gcaaggcctt cgagggcgac ccggagagaa
     1681 agcgcaaggt cctctcccga accccgatga acgagctggg gctccccgag gacgtggcga
     1741 acgccgtcgt cttcctcgcc tctcccgcgg cgcgctatct taccggcgtc gtccttcccg
     1801 tggacggcgg cacgagcatc ggattctaat ctgttgctta ctgcagagag caaaagagag
     1861 gaggtttgcg catgattctg tcattcagat ggtacggaga aagcgacccc gtcactctcg
     1921 acaagatccg acaggttccg gtgcttaaag ggatcgtcag cgcgctctac gacgttccgc
     1981 tcggcgagac ctggccgctg gagaagattg agagcctgaa agcggcggtt gagcggaagg
     2041 ggttcgtcct ctccgcgatc gagagcatcc cggtgcacga gaatatcaag ctggggcgtc
     2101 ccgacagaga tgtccttatc gacaagtact gcgcctccat ccgcaacatg gggaaggccg
     2161 gtattccggt gctgtgctac aacttcatgc cggtcttcga ctggacgagg tcgaacatgg
     2221 cgtttccgct gccggacggc tccaacgccc tttcgtacga cgacgtcgaa gtgctcaaga
     2281 tggatctctc gaaggggacg a
//
LOCUS       DAOPKU010000492         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_54334_length_2300_cov_3.608463, whole genome shotgun sequence.
ACCESSION   DAOPKU010000492 DAOPKU010000000
VERSION     DAOPKU010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54334_length_2300_cov_3.608463"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..942)
                     /locus_tag="PK849_12675"
     CDS             complement(58..942)
                     /locus_tag="PK849_12675"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPE67027.1"
                     /translation="MTEDMRPATSRETRLPEAKGFSKRRTRSIDGVLGAVAGDVIGSV
                     YEWHAIKTETFPLFSPSSRFTDDSIMTVATADALLSDGDYARAYRSWGRAWPDAGYGG
                     NFSRWLRKAHASPYGSFGNGSAMRASPAAYAADSLEEVLRIAEATSVVTHDHPQGIIG
                     AQALTGSVFLARRAASNGTVASCKEEIRAFLERRCGYSLRRTVAEIRPDYTFDVTCQG
                     SVPESLICFLEASDFESAVRKAVSLGGDADTQACIAGAVAGALWGVPEHIWKRVRSTL
                     DASMLRILDAFEERFAKE"
     gene            complement(1348..1863)
                     /locus_tag="PK849_12680"
     CDS             complement(1348..1863)
                     /locus_tag="PK849_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67028.1"
                     /translation="MKIIEGMKRLRVIEKRMESQRNAVTEYASKLSTEMPRFQTKEDQ
                     AKEVASLIQSNNDLCAEYLRIKRSIEYTNLKVTVELQGKAYSISDLLVVKRKLADRMV
                     ATYRALNDTVARDRLRNAPKFDGETPKVEILYDERTKLDNIRKWQDLADIIDSRLEVI
                     NATTDLIEMEE"
BASE COUNT          460 a          668 c          657 g          515 t
ORIGIN      
        1 acaaaaaaag agaccttttc aggcctcttc aggggtgttc aactgtacgg tcgcatgtta
       61 ttccttcgcg aagcgctcct cgaaagcgtc cagaattcgg agcatggatg cgtcgagcgt
      121 gctccgcacc cgcttccata tatgttccgg cacgccccaa agggctcccg ctacagcgcc
      181 ggcgatgcat gcctgcgtat ccgcatcccc gcccagcgag acggctttgc gaacggcgct
      241 ctcgaagtcc gacgcctcaa gaaaacagat gagagattcc ggcacgctcc cctggcaggt
      301 gacgtcgaac gtgtagtcgg gacggatctc ggcgacggtc ctccgcagac tgtatccgca
      361 gcggcgttca agaaaggcgc ggatttcctc cttgcaggac gccacggtcc cgttcgatgc
      421 ggccctgcgg gcgagaaaga cgctgccggt aagcgcctgc gctccgatga ttccctgagg
      481 atggtcgtgc gtcacgacgg aagtcgcttc cgcgatgcgc agcacctctt caagagaatc
      541 ggcggcgtac gcggcggggc ttgcgcgcat cgcggagccg tttccgaagc ttccgtaggg
      601 cgacgcgtgc gcttttctca gccaccgcga gaagttgccg ccgtatcctg cgtcgggcca
      661 cgcgcggccc caggaacgat acgcgcgggc gtagtctccg tcggaaagca gcgcatcggc
      721 cgtcgcaacg gtcatgatgc tgtcgtcggt gaaacgggaa gagggactga agaggggaaa
      781 cgtttccgtc tttatggcgt gccactcgta caccgagccg ataacatctc ccgcaacggc
      841 tcccaatact ccgtcgatcg aacgggtccg gcgctttgaa aacccctttg cttccggaag
      901 ccgtgtttcc cgagaagttg ccgggcgcat gtcttctgtc atgcaatcac cttcctcgca
      961 taaaagatag aggaaaaaaa gagaagcggc gacagccggg ggtgagaatg cccgggaaag
     1021 aacgggcgca cctgttccgg atttttccgt caaacgtccg gcccgcgaag gccgtgacat
     1081 cggtttcttg agacttcact cttgagtcgt gatcccctgg ttaccgacgc ggggccgtct
     1141 taatcttgag tcgtcagtcg tgagccttga ggcgcatacg ctaggagcgt atcatgcgtc
     1201 gcctgccgct tcgcgaatct gaaggttcgt cttgaccccg acaagggtct ttcccaattt
     1261 tctttttaca tcgccgcttc gtccttattc tcatgtcaag gtgccgggtg tttcaggctg
     1321 cgcggaggga atcgcctccg cgccttttta ctcttccatt tcgatcagat cggtcgtggc
     1381 attgatgact tcaagcctcg aatcgattat atcggccaaa tcctgccact tccggatatt
     1441 atccagtttt gtccgttcgt cgtaaagaat ctccaccttc ggagtctcgc cgtcgaattt
     1501 cggagcattc cgcagacggt cccgggctac cgtgtcgttc agagcgcggt aggtggccac
     1561 catcctgtcg gcaagttttc tcttgaccac aagcaggtcg gagatgctgt acgcctttcc
     1621 ctgaagttca acggtcacct tcagattcgt gtactcgatc gaacgtttga tcctgaggta
     1681 ctccgcgcac agatcgttgt tcgactggat aagagacgcc acctctttcg cctggtcttc
     1741 cttcgtctgg aaacgaggca tctccgtgga gagtttcgag gcatactcag taaccgcatt
     1801 ccgctgggat tccatacgct tttcaatgac tctcagtcgt ttcattcctt cgattatctt
     1861 catggccgag ccctccccgg tgtatttgga ttcgagattc cgcgggggaa cctcggtgcg
     1921 tccgacaagt gaaagtatag cacctttcgg cgaccgttta cgtcgtcgcc ccgaacgcag
     1981 gggaaatgat tatttcgccg ttttcgggga aggagccggt aggttggaac ggaatcccgg
     2041 ccccgcaaca cgtaagcggt cgccctgaat atgtatcatg gctgtgttgt cgatggacga
     2101 ggcagtacga aaggggatgt tctcatgacg aattcaaaag gccggaacac gcatgaagaa
     2161 ttcatgaacc gggaaactcc gcgcgcttcc ttggaagcca aggcgaatac cccgctctcc
     2221 gagctgaaaa gcatgcccgt gtggcaagcc gtcggggtct gcgaagatcc ggagacgttg
     2281 aaaaagctgc tggaatcggg
//
LOCUS       DAOPKU010000493         2298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_54408_length_2298_cov_4.074008, whole genome shotgun sequence.
ACCESSION   DAOPKU010000493 DAOPKU010000000
VERSION     DAOPKU010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54408_length_2298_cov_4.074008"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PK849_12685"
     CDS             <1..1113
                     /locus_tag="PK849_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE67029.1"
                     /translation="NMGVEGGGVNPMRGQNNVQGACDMGALPNMYCGYQKVDSPELNK
                     KFADFWGVGALPSKPGLKIGEMVNGLPEGKLKAFWIFGENLVATEPDPRHVSHCLNSA
                     EFLVVQDIFQNETTPFADVILPSAAWCEDEGTFTNTERRVSRVRKIKEAPGEAKPNWW
                     IFKQIAKRFGYEWSSTGGQEIWDNEIAALCPAFAGIKFRRLDSIGGIQWPCPTEEHPG
                     TPILHKDGKFSRGKGLFQATEWVPSSEQPDEEYPMVLSTGRRLVHYHSRTQTGRAKGM
                     NERMPDERADISVQDAERLGIAHGEEIRVTSRRGEVRIKANVSKRIAPGMVWMSFHFW
                     ETNANHLLSGDLKTFDPDTMTPSFKACAVRIEKIAG"
     gene            1110..1715
                     /locus_tag="PK849_12690"
     CDS             1110..1715
                     /locus_tag="PK849_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HPE67030.1"
                     /translation="MNYGRFASYEEFLNELTLFHGKPAPGGVLALHMVNMAWEVFPKD
                     VLMDVICETRKCLADTIQLLTPCTVKNHWLKIVDTGRFAGVFYDKQTGEGVRISLSME
                     RLKLWPRVEEWYLKLTPKHEQSLQAILDEINEAGAELFDMVEVTVEPEVLKVRPKVPP
                     VFCPICGEAYPPDHGPICRGCAGLTDSYYRSRTPAAAGAER"
     gene            complement(1795..>2298)
                     /locus_tag="PK849_12695"
     CDS             complement(1795..>2298)
                     /locus_tag="PK849_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67031.1"
                     /translation="LFEILNPETLTPTLPGEFGEIVVTTLRTEGTPLLRYRTGDRGRF
                     LSGTCRCGSILKRIETTGRLRNGITLHNGVFVPLHELDEALFSIPWLADYSASQEDGY
                     ITVSLHAPSREATTPEPAPQEHAALAAALDNLSADAKSALQKRPFRFRFLPHAHDGVS
                     SRKRIAG"
BASE COUNT          446 a          701 c          764 g          387 t
ORIGIN      
        1 aacatgggcg tcgagggcgg cggcgtcaat ccgatgcgcg gacagaacaa cgtgcagggc
       61 gcctgcgaca tgggcgcgct tcccaacatg tactgcggct accagaaggt ggattcgccc
      121 gagctgaaca agaagttcgc cgacttctgg ggcgtcggag cgctgccctc gaaaccgggg
      181 ctcaagatcg gagagatggt caacggtctg cccgagggca aactgaaggc cttctggatc
      241 ttcggcgaga accttgtcgc caccgaaccg gatccgcggc acgtctcgca ctgcctgaac
      301 tccgctgagt ttctcgtggt gcaggatatc ttccaaaacg agacgacgcc cttcgcggac
      361 gtcattcttc cctcggccgc atggtgcgag gatgaaggca ccttcacgaa caccgagcgg
      421 cgggtgagcc gcgtgcgcaa aatcaaagag gctcccggcg aggcgaagcc gaactggtgg
      481 atcttcaagc agatcgccaa gcgcttcggc tacgagtggt cgtcgacggg cggtcaggaa
      541 atctgggaca acgagatcgc ggcgctctgc ccggccttcg cggggatcaa gttccgacgc
      601 ctcgacagca tcggcggcat ccagtggccc tgcccgacag aggaacaccc cgggacgccc
      661 atcctccaca aggacggcaa gttctcccga ggaaaaggtc tcttccaggc cacggagtgg
      721 gttcccagca gcgagcagcc cgacgaggag tacccgatgg tgctcagtac cggccgtcgt
      781 ctggtgcact accactccag gacgcagacg ggccgcgcga agggaatgaa cgagcgtatg
      841 cccgacgagc gcgccgacat ctccgtgcag gacgccgaga ggctgggcat cgcccacggc
      901 gaggagatcc gcgtgacgtc gcggcgcggc gaggtacgca tcaaagcaaa cgtctccaag
      961 cgcatcgctc cgggaatggt ctggatgtcc ttccacttct gggagacgaa cgccaaccat
     1021 cttctgagcg gcgacctcaa gaccttcgat cccgacacca tgacgccctc cttcaaggcg
     1081 tgcgccgtaa ggatcgagaa gatcgcggga tgaactacgg aaggttcgcg tcgtacgagg
     1141 agttcctgaa cgaactgacg ctgtttcacg gaaaaccggc gcccggcggc gtcctcgcgc
     1201 tgcacatggt gaacatggcc tgggaggtct tccccaagga cgtgctgatg gacgtgatct
     1261 gcgagacgcg gaagtgtctc gcggacacta tccagctctt gacgccctgc accgtgaaaa
     1321 accactggct gaagatcgtg gatacaggac ggttcgccgg cgtcttttac gacaagcaga
     1381 ccggcgaggg ggtccgcatc tcgctaagca tggagcggct gaagctctgg ccgcgcgtcg
     1441 aggagtggta cctgaagctc actccgaaac atgagcagag ccttcaggcg atcctcgacg
     1501 agatcaacga ggcgggcgcc gaactcttcg acatggtcga agtaaccgtg gaacccgaag
     1561 tgctcaaggt gcgcccgaag gtcccgccgg tcttctgccc gatctgcggc gaggcctacc
     1621 cgcccgacca cggcccgatc tgccgggggt gcgcgggact caccgactcc tactaccgca
     1681 gccgcacgcc cgcagcggca ggcgccgaac gctgaacaca cgaagaggga gggctcgccg
     1741 agccctccct cttcgtgtct tccttccttt tgtttcttga agaagtcaca cctctcatcc
     1801 cgcgattcgt ttcctgctgg agacgccgtc gtgggcgtgg gggaggaagc ggaagcggaa
     1861 cgggcgtttc tgcagggcgg acttcgcatc ggctgagaga ttgtcgagcg cggcggcgag
     1921 cgctgcgtgt tcctgcgggg cgggttccgg cgtggttgct tctctcgaag gggcgtggag
     1981 ggagaccgta atgtatccgt cttcctgcga cgccgagtag tcggccagcc aggggatgga
     2041 gaagagcgct tcgtccagct cgtgcagcgg aacgaagacg ccgttgtgga gggtgatgcc
     2101 gtttcggagt ctccccgtcg tttcgattcg tttgaggatg gagccgcagc ggcacgttcc
     2161 ggagaggaag cgccccctgt cgccggtgcg gtagcggagg agcggagtcc cctccgtacg
     2221 cagcgtggtg acgacgatct ccccgaattc cccgggaagc gtgggggtga gcgtctccgg
     2281 gttcaggatc tcgaagag
//
LOCUS       DAOPKU010000494         2290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_54648_length_2290_cov_3.779418, whole genome shotgun sequence.
ACCESSION   DAOPKU010000494 DAOPKU010000000
VERSION     DAOPKU010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54648_length_2290_cov_3.779418"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="PK849_12700"
     CDS             <1..1434
                     /locus_tag="PK849_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPE67032.1"
                     /translation="PPGDGRIIFTAPIKALSNERYLDLRKLGLDVGIETGDFKKNEGA
                     GIICCTQEIYSLKYARVPGQKLIVDEFHYIFDDSDRARTYIDGIRNTDATTSVLVMSA
                     TLGGASSVVKYLEGVTERSFLLFENAKRETQLLFTPERPAYIDSVKDALVFLFSQKGA
                     MELAYLIARQRKRIHSSKRERLAELAAILGVPKIQQPLFNGVGIYHGGMLPKEKILME
                     AAFRERILDVVCGTNALALGVNLPAETAVFAQLVHYHSDAPISKNEFLQMAGRAGRKG
                     LFDPGYVTWLAKSPFERRGFSTKEIFNELLEAAPEKANVSLRPSFGRLLRKEVLPEVE
                     AEFIARYSLPQQDEYLVGNSIRSGMKRIDRAMSKMVHHHERKKFRKTLASLWYDEMDI
                     EENLEMAFLFHTEQSPSALLAAQVILPFERNYLQALLKVKRFANRMPKGFAFRSMKEL
                     NNTVDEIDPTIYGFEEKIGEIERSLFS"
     gene            complement(1454..>2290)
                     /locus_tag="PK849_12705"
     CDS             complement(1454..>2290)
                     /locus_tag="PK849_12705"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPE67033.1"
                     /translation="GEGAYVLARQAALLTEEVTRAPQILLCLGRVRDSDEYTFVHSLN
                     VGLLCGYLAGRLKPGDREFAASMTYGGLLHDLGKAKVPQQVLNKPGKLTEKEYEMMKA
                     HSIYGDEIARSSGISDVRVLSVIRNHHERWGGHGYPDGLQKNSIPLFARIAAVADVFD
                     ALTAKRVYKNPLSSREAVSMILESSENDFDKGVVRELLLSVGLYPAGTLVELSDFSVG
                     VVVGARNTDLFRPQVSVTIDGKGRRAPEGTIVDLGLQQDLFVRRALDDVGKGVAYSEK
                     AG"
BASE COUNT          496 a          690 c          645 g          459 t
ORIGIN      
        1 cctcccgggg acggacgcat catcttcacc gccccgataa aggcgctgag caatgaacgc
       61 tatctcgatc tcaggaaact cgggctcgac gtgggaatcg aaaccggcga cttcaagaag
      121 aacgaaggcg caggcataat ctgctgcacg caggagatct attccttgaa gtacgcccgc
      181 gtccccggac aaaaactcat agtggacgag ttccactaca tcttcgacga ttcggaccgg
      241 gcgagaacct atatcgacgg catccggaac accgacgcca caacttccgt cctcgtcatg
      301 tccgcaaccc tcggaggcgc ctcctcggtc gtaaaatacc ttgaaggagt gactgaacgc
      361 tcgtttcttc tcttcgaaaa cgcgaagcgc gagacgcagc tcctcttcac gccggaacgc
      421 cctgcgtaca tcgacagcgt caaagacgcg ctggtcttcc tcttttcaca aaagggggcg
      481 atggagctcg cctacctgat cgcccgccag aggaagcgga ttcattcctc caagcgcgaa
      541 cgcctcgctg aacttgcggc gattctgggc gtgccgaaga ttcagcagcc gctcttcaac
      601 ggggtcggca tctaccacgg cggaatgctg ccaaaggaaa aaatccttat ggaggcggcc
      661 ttcagagagc gaatcctcga cgtggtctgc ggaacgaacg cgcttgcgct tggcgtcaac
      721 ctccctgccg aaaccgccgt atttgctcag cttgtccact accactcgga cgcgccgatc
      781 tcaaagaacg agtttcttca gatggcggga cgggcaggac ggaaggggct cttcgacccg
      841 gggtatgtca cgtggcttgc aaaatcgccg ttcgagcggc gggggttttc aacgaaggag
      901 atcttcaatg agctgcttga ggcggccccg gagaaggcga acgtctcgct gcgcccgtct
      961 ttcggcaggc tgctccgcaa agaggtcctc cctgaagtgg aagcagagtt catcgcacgc
     1021 tactccctgc cgcagcagga cgagtatctc gtcgggaact cgatccgctc cggaatgaag
     1081 cgtatcgaca gggcaatgtc gaagatggtc caccaccacg agcggaagaa gtttcgaaag
     1141 acgcttgcct cgctctggta cgatgagatg gacattgagg agaacctgga gatggcgttt
     1201 ctttttcaca cggagcagtc gcccagcgct ttgctggccg cgcaggtgat ccttcccttt
     1261 gaaaggaact acctccaggc gctgctcaag gtgaaacgat ttgcgaacag aatgccgaaa
     1321 gggtttgcct tccggtcgat gaaagagtta aacaatacgg ttgacgaaat cgatccgacg
     1381 atctacgggt tcgaggagaa gataggagag atcgaacgat cgctcttctc ctgaattttg
     1441 agtgcaagcg tctttaccct gctttttcgc tgtacgcaac gcccttccct acgtcgtcga
     1501 gcgcccggcg gacaaagagg tcctgctgca atcccaggtc gacgatggtt ccttccggag
     1561 cgcggcgccc cttcccgtcg atggtgacac tcacctgggg gcggaagaga tccgtatttc
     1621 tggcgccgac caccactccc acgctgaaat ccgaaagctc gaccagcgtg ccggccggat
     1681 agagtcccac ggagagcagc aactcccgga cgacgccctt gtcgaagtcg ttttcggagg
     1741 attccagaat catcgatacc gcttcgcggc tcgacagcgg atttttgtac actctctttg
     1801 ctgtgagcgc gtcgaagaca tcggcaaccg ccgcgatgcg ggcaaacagc gggatactgt
     1861 tcttttgcag accgtcgggg tacccatgcc ctccccaacg ctcatggtga ttccggatga
     1921 cagaaagcac gcggacgtcg ctgataccag atgacctggc gatttcatct ccgtagatgg
     1981 agtgcgcctt catcatctcg tactccttct ccgtcagttt tcccggcttg ttgagcacct
     2041 gctgcggaac cttggcttta ccgaggtcgt gaaggagtcc gccgtacgtc atcgaggcgg
     2101 cgaactcgcg gtcgccgggt ttcagacgtc cggcaagata tccgcagagc agccccacat
     2161 tgagggagtg gacgaatgtg tactcgtcgc tgtcgcgcac ccgccccaaa cagagcaaaa
     2221 tctgcggagc gcgcgtaacc tcttcggtca acaacgccgc ctgtctcgcc agcacgtagg
     2281 cgccttctcc
//
LOCUS       DAOPKU010000495         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_55289_length_2272_cov_4.396031, whole genome shotgun sequence.
ACCESSION   DAOPKU010000495 DAOPKU010000000
VERSION     DAOPKU010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55289_length_2272_cov_4.396031"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK849_12710"
     CDS             complement(<1..423)
                     /locus_tag="PK849_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE67034.1"
                     /translation="MTVKYLRVVNRDQCIGCFSCMYACSRMVHNAAGSGKAALRVKAY
                     TGVEGAFSIRVCARCEEPDCAAACPTGALTKAPGGGVRLKKELCISCMACVKACAIGA
                     LQWDEEEKRPLPCIHCGQCVKYCPNNVLALCEKEEECEP"
     gene            548..877
                     /locus_tag="PK849_12715"
     CDS             548..877
                     /locus_tag="PK849_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67035.1"
                     /translation="MRKIVLIAVLWGAMFGSAFAEPAPAAWKGDLRHVVERGGVSYSI
                     YADRTRLVEDCVPGAEQVLETFVHLVIESQNLVEIQQWNIRQDGSGYRVQDMYDYTLD
                     TFGMVDQ"
     gene            1038..1607
                     /locus_tag="PK849_12720"
     CDS             1038..1607
                     /locus_tag="PK849_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HPE67036.1"
                     /translation="MEKEKLFHRMLDVIEGDIVPLTARGVSIGSKVFGAAVLRRDDLS
                     LVVAGTNHEAFSPLWHGEVYTIKLFYELQGRPDPSECLFLSTHEPCSMCISALSWAGF
                     KDIYYFFGYEQTQDDFNIPHDLRMLREVFGCATPTKENIYYTSRSLMDIAEGLEDLVT
                     ARARIARIKETYAGLSAVYQAGEKKMVLK"
     gene            complement(1718..>2272)
                     /locus_tag="PK849_12725"
     CDS             complement(1718..>2272)
                     /locus_tag="PK849_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPE67037.1"
                     /translation="FDDIRGKYGTVDYDDLMAFTDWAVEHLPFVDKDRLGVTGGSYGG
                     YMTNWIIGHTDRFKAAASQRSISNWISKMGISDIGYYFVPDQQAADIWSDHEKLWFHS
                     PLKYADRAKTPTLFIHSEEDHRCELTQGLQMFTALKLHGVETNICVFKGENHELSRSG
                     RPRPRLTRLRKMTEWFDRFLKAKE"
BASE COUNT          417 a          629 c          735 g          491 t
ORIGIN      
        1 cggttcacac tcctcttctt tctcgcagag cgcgaggacg ttgttggggc agtatttaac
       61 gcactggccg cagtggatgc aggggagggg gcgcttctcc tcctcgtccc actggagcgc
      121 gccgatcgcg cacgccttga cgcacgccat gcaggagatg cagagctctt ttttcaggcg
      181 cactccgccg cccggggcct ttgtcagcgc tcccgtcgga catgccgcgg cgcagtctgg
      241 ctcctcgcac cgtgcgcaga cgcggattga gaatgcgccc tccacgcctg tgtaggcttt
      301 gacccgcagc gccgccttgc cgctgcctgc ggcgttatgc accatgcggc tgcacgcgta
      361 catgcagctg aagcagccga tgcactggtc acggttgaca actcgtagat atttgacagt
      421 catgcgttcc acctctcact ttttttctct ctccgcaata atcccctgtc tttttctccc
      481 tgtcaactgc tacaatgaac aaagagaaaa gaattcgagc cctttcgaat aaacggaggt
      541 gggagcgatg aggaaaattg tgttgatcgc cgtcctttgg ggcgcgatgt ttggaagcgc
      601 gtttgcggag ccggctcccg cggcgtggaa gggagatctg cggcatgttg tggagcgcgg
      661 cggggtctcc tattccatat atgcggacag aacccgtctg gtggaggact gtgttccagg
      721 cgcggagcag gtgctcgaaa cgtttgtgca tctggtgatc gaatcgcaga acctggtcga
      781 gatccagcag tggaacatac ggcaggatgg aagcgggtac cgcgttcagg atatgtacga
      841 ctatacgctg gatacgttcg gcatggtgga tcagtgaatc gtcctgcccg agtgggcctc
      901 gtggaggccc gtggaggagg actcgtatca gcgggatgtt atccgggctg cacagatgat
      961 tgtagcggag cgaggcgatt gactctgcgc cggaatccgt tcatgcgttc cgatactctt
     1021 tgaagggagc tgtttttgtg gagaaagaga agctgtttca tcgtatgctc gacgtcatcg
     1081 agggggatat cgttccgctg acggcgcggg gggtgagcat cgggagcaag gtgttcggcg
     1141 cggcggtgct gcggagggac gatctctcgc tggtggttgc gggaacgaat cacgaagcct
     1201 tctccccgct gtggcacggc gaggtgtaca ccatcaagct cttctacgag ctgcaggggc
     1261 gcccggaccc ttcggagtgc ctcttcctct cgacgcacga gccgtgctcg atgtgcattt
     1321 cggcgctgtc gtgggcagga ttcaaagaca tctactactt cttcggctac gagcagacgc
     1381 aggacgactt caacatcccg cacgacctgc ggatgctgcg cgaggtcttc ggctgcgcaa
     1441 cgccgacgaa ggagaatatc tactatacca gccgttcgct catggatatc gcggaggggc
     1501 tggaggatct ggtgacggcc agggcgagaa tcgccaggat aaaggagacg tatgccgggc
     1561 tgtccgccgt ctaccaggcg ggcgagaaga agatggtgct taagtagcgc gggatgtgca
     1621 agatgcgcgt cggcggtaca atccgatgaa cgaaaaatcc cccctgcgcc gcgaggatgc
     1681 aggggggatt tgcgttcgag tcgcggaact tcctccgcta ttctttcgct ttcaaaaagc
     1741 ggtcgaacca ctccgtcatc ttgcgcagtc gggtaagtct ggggcgcggg cgtccgctcc
     1801 ggctgagttc gtgattctcg cccttgaaga cgcagatgtt cgtctccacg ccgtggagct
     1861 tcagcgccgt gaacatctgg agcccctggg tgagttcgca gcggtggtcc tcctcggagt
     1921 ggatgaagag cgtcggagtc ttcgccctgt cagcgtactt cagcggtgag tggaaccaga
     1981 gcttctcgtg gtcgctccaa atatcggcgg cctgctggtc ggggacgaag tagtagccga
     2041 tgtccgaaat ccccatcttt gagatccagt tgctgatgct ccgctgcgag gcggcggcct
     2101 tgaaacggtc ggtgtgcccg atgatccagt tcgtcatgta ccccccgtag gagccgccgg
     2161 tgacgccgag ccggtctttg tcgacgaagg gaaggtgctc cacggcccag tcggtgaagg
     2221 ccatcaggtc gtcgtagtcc acagtgccgt acttcccgcg gatgtcgtcg aa
//
LOCUS       DAOPKU010000496         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_55291_length_2272_cov_4.377988, whole genome shotgun sequence.
ACCESSION   DAOPKU010000496 DAOPKU010000000
VERSION     DAOPKU010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55291_length_2272_cov_4.377988"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PK849_12730"
     CDS             <1..976
                     /locus_tag="PK849_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE67038.1"
                     /translation="SGTGLGLAISRQFAELMGGSIGVTSEEGNGSEFWFTVRLEKQPE
                     GACRDEVHRHAAATAPRVERRGVRVLLAEDNAVNRKVALGMLKKLGFTADSVADGEQV
                     LSALSERTYDLVLMDCQMPVMDGYEAARRIRALEKPGGKRIPVVAMTAYAMSGDRERC
                     IEAGMDDYLAKPISIEALSAALASWLPHREGPGGTDGSPRRSEPPTPREEAETCSKSA
                     VWRKDELLNRLLGDEEMTREILEAFLEDIPREIDSLTAAVAVGNTPTVELLAHTIKGA
                     AGNVGSEGLRSAAFEMESAAKKGDLSGAQALLKSIRQEFEHLKAAIRG"
     gene            complement(1093..>2272)
                     /locus_tag="PK849_12735"
     CDS             complement(1093..>2272)
                     /locus_tag="PK849_12735"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE67039.1"
                     /translation="FLSSSRPHYIVSEGPEAGVYVRLGSTNRQADRELIAELHRSVEG
                     VSFDELPMPHLTVDDLAMNSIKSSFEDIREVDEKTLLTLRLLIKEQGRNVPTKGGILL
                     YGRDRPAHFPDCWAQCGRFIGVDKTRIFDHIELEGPLPSLVDSIMLFLKKHAMRGADF
                     SEVRRKDVWSIPIEPLREAVVNAVVHADWSQRGAPVRVAFFDDRIEIENPGILPPGMT
                     VEDMRAGVSKIRNPVIARVFRELKLIEQWGSGIPRIFRETAAGGFPEPYFIELGLRLR
                     FVFPLTKPLKIDEEQPAPQSEERLESRLESRLESRLESRLAAKVVLLIESQEAGKTEL
                     ARELGHKSVSGELHKQIRRLIDLDWIEMTVPEKPNSRLQKYRLTDEGKKALDIEKEQK
                     "
BASE COUNT          478 a          666 c          691 g          437 t
ORIGIN      
        1 cagcggcacg ggtctcggcc ttgcgataag caggcagttc gccgagctga tgggaggctc
       61 catcggggta acgagcgaag aggggaatgg ctcggagttc tggttcacgg tccgtttgga
      121 gaagcagccg gaaggcgcct gccgcgacga ggttcaccgc catgccgccg cgaccgcccc
      181 gcgcgtggag agaaggggcg tgcgcgtcct gctcgcggag gacaacgccg tgaacagaaa
      241 agtcgccctg gggatgctga aaaagctcgg gttcacagcc gactccgtcg cggacggaga
      301 acaggttctc tccgcgcttt cggaacgcac gtacgatctt gtgctcatgg actgtcagat
      361 gcccgtgatg gacggctacg aggcagcccg ccgcatccgc gcactggaga agcccggcgg
      421 aaagcgaata ccggttgtgg cgatgaccgc gtacgcgatg agcggcgacc gggaacgctg
      481 catcgaggcg ggaatggacg actatctcgc aaagccgatc tcgatcgagg cgctgtcggc
      541 cgcccttgcg tcttggttgc cgcacaggga aggacccggc ggaacggacg ggagcccccg
      601 ccgatccgag ccgccgacgc ctcgggaaga agcggaaacg tgctcgaaaa gcgccgtctg
      661 gagaaaggac gagctgctga acagacttct gggagacgag gagatgaccc gcgaaatcct
      721 ggaggcgttt cttgaggata ttccccggga gatagattcg ctcaccgccg ccgtggccgt
      781 gggcaacaca cccaccgtgg agctgcttgc tcacaccatc aagggcgcgg cggggaacgt
      841 gggaagcgag gggctgcgct cggcggcctt cgaaatggag tcggccgcca agaagggcga
      901 tctgtccgga gcgcaggcgc tcctgaagag catacgacag gagttcgagc acctgaaagc
      961 ggcgattcga ggataaacgc gcgccccgct acgccaaacc ggccggttcg tagacgggag
     1021 agaaaagtcc cggatggcct gccgcaaggc gtcgcgggtg gtctccttgt ccttcggatg
     1081 aatcgaactg tttcactttt gttctttctc tatatccagc gccttttttc cttcatcggt
     1141 caggcgatac ttctgcaacc gactgttcgg cttttccggg acggtcattt cgatccagtc
     1201 aagatcaatc aaccgcctga tctgcttgtg aagctcaccc gagacgctct tatgacctag
     1261 ttcccgcgct aattccgtct ttcctgcttc ctgagattcg atcaacaaga cgactttagc
     1321 cgccagccgc gactctagcc gtgactctag ccgtgactct agccgtgact ctagccgttc
     1381 ttccgactgt ggcgcaggct gctcttcgtc aatcttcaac ggtttcgtca gcggaaaaac
     1441 aaaacgcagc cgcagaccca gttcgatgaa atacggctcg ggaaaccctc cggcagcagt
     1501 ttcccggaag atgcggggaa taccggatcc ccactgttcg atcagtttca actcccgaaa
     1561 gacgcgcgca atcaccggat tgcgtatttt ggaaactccg gcccgcatgt cctcgacggt
     1621 cattcccgga ggcagaatgc cggggttttc gatctcgata cggtcgtcaa agaaagcgac
     1681 acgtaccgga gcgcccctct gggaccagtc ggcatgcacc acggcgttga caaccgcttc
     1741 gcgcaaaggc tcaatgggta tgctccagac atcctttcga cgcacttcgg aaaaatccgc
     1801 gccgcgcatg gcatgcttct tcaggaagag catgatggaa tccaccagcg acggcaacgg
     1861 gccttcgagt tctatatggt cgaatatccg ggttttatcg acgccgatga aacggccgca
     1921 ctgagcccag cagtcgggaa agtgagcagg acgatctcta ccgtaaagca ggatgccgcc
     1981 tttggtcggt acatttctcc cttgctcttt gatcagcagt ctcagagtga ggagcgtctt
     2041 ttcgtcgact tcgcgaatgt cttcaaagga actcttaatc gagttcatcg caagatcgtc
     2101 cacggtaagg tggggcatgg gcaattcatc gaacgagact ccctcgactg aacggtgaag
     2161 ctcggctatc aactcgcggt ccgcctggcg gttcgtggag cccaggcgaa cgtatactcc
     2221 tgcctcggga ccttcggaga ctatgtagtg cgggcgcgaa ctgctcaaga ac
//
LOCUS       DAOPKU010000497         2270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_55353_length_2270_cov_4.598194, whole genome shotgun sequence.
ACCESSION   DAOPKU010000497 DAOPKU010000000
VERSION     DAOPKU010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55353_length_2270_cov_4.598194"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK849_12740"
     CDS             complement(<1..387)
                     /locus_tag="PK849_12740"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67040.1"
                     /translation="MKRRVFLSFAVLVCVIFAAAGAGMQAEASDYKLVLRLSHVFHPD
                     EQLTKSMDWVAERILKRTNGAIEIQTFPQGQIAVYKDGMEQVVRGANFISVEDPSYVG
                     DYVPDFIALVGPMLYNSYDEYVEIIKT"
     gene            complement(511..768)
                     /locus_tag="PK849_12745"
     CDS             complement(511..768)
                     /locus_tag="PK849_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPE67041.1"
                     /translation="MEKQVVVRNPHGLHARPAAKFVQAASSVPEDVIIEKNGKTANAK
                     SILSIMSLGVSKGDTVTLRVAGESGEALLQTLEGILVSEAE"
     gene            complement(768..1151)
                     /locus_tag="PK849_12750"
     CDS             complement(768..1151)
                     /locus_tag="PK849_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HPE67042.1"
                     /translation="MEERLLNMICVGCPVGCDIKVAVEDTKVLSVEGNNCPRALEFAK
                     AEVANPTRVFATTVRVSGGKLPVCPVRSRQAVPKNRLFDISREVARLVVPAPVEVGQV
                     ILPDACGTGVDIVASRDLKTEEESA"
     gene            complement(1157..>2270)
                     /locus_tag="PK849_12755"
     CDS             complement(1157..>2270)
                     /locus_tag="PK849_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE67043.1"
                     /translation="IHPGFGLHTFKEELTGPEYMHRWIKRAHEAGVQFLVNSMVFKME
                     PDGSLWVMNPEKGIFRVVPKATVLAMGCRERPLGAINVPGSRPAGIYTAGTAQRYVNM
                     EGYMPGHKAVILGSGDIGLIMARRMIWEGASVEGVYEIMPWAGGLRRNIAQCLDDYGI
                     PFYLEHSVVKVHGHDRLEGITVVKVDENRRPIPGTDRFVACDTMLLAVGLIPENELSR
                     MAGVEIHPVTGGPVLNEFYQTTRPSVFAAGNVVIVYDLADWVSQEGFAAGRNAALYSA
                     GTLRSSERRIAIKGGKNVRLFSPQFITGCCDATVYFRVTHPIEKRCRVFAEHGLFEQK
                     HRYVRPGEMNEVHLKGAALQALPESVAEITIDAEEV"
BASE COUNT          400 a          720 c          689 g          461 t
ORIGIN      
        1 ggtcttgatg atctcgacgt actcgtcgta ggagttgtaa agcatcgggc cgaccagcgc
       61 gatgaaatcg ggaacgtagt cgccgacata ggaggggtcc tcgaccgaga tgaagttcgc
      121 tccgcggacg acctgctcca tgccgtcctt gtagacggcg atctgcccct gggggaacgt
      181 ctggatctcg atggcgccgt tcgtcctctt gaggatccgc tccgccaccc agtccatcga
      241 cttggtgagc tgctcgtccg gatggaagac gtggctcaga cgcagcacca gcttgtagtc
      301 ggaggcttcc gcctgcattc cggcccctgc cgccgcaaag atgacgcaca ccagcaccgc
      361 gaaactcaaa aacactctcc gtttcattcg caacactctc cttcagtatg ataggacgct
      421 tcctccgggc agtccgccgc aaccggcgcg aaccgcccgt attcatatct atcgtctttc
      481 cgggctcctg tcaatcgctt ttctcagaaa ctactccgcc tcgctcacca aaattccttc
      541 gagggtctgc agcagggctt cgccgctctc tccggcgacc ctcagggtga ctgtgtctcc
      601 cttggagacg ccgagggaca taatagagag aatgctcttc gcgttcgcag tcttcccgtt
      661 cttttcgatg atcacgtcct ccgggacgga cgacgccgcc tggacgaact ttgctgcggg
      721 gcgggcgtgc agcccgtggg gattgcggac aacgacctgt ttttccatta tgcggactcc
      781 tcctcggtct taaggtcgcg gcttgcgaca atgtcgacgc ccgtcccgca ggcgtcgggg
      841 agaataacct gtccgacttc tacgggagcg ggaacaacga ggcgcgctac ctccctggag
      901 atatcgaaca gacggttttt cggcactgcc tggcggctcc tgacggggca gacgggcagt
      961 tttcccccgg aaacgcgcac cgtcgtcgcg aagactctcg tcgggttggc cacctcggcc
     1021 tttgcaaact caagcgccct cgggcagttg ttcccctcga cggagagaac cttcgtatct
     1081 tcgaccgcaa ctttgatgtc gcacccgacg gggcagccaa cgcagatcat gttcagcaga
     1141 cgttcttcca tggcgatcag acctcctcgg cgtcgatggt gatttccgcg acggactcgg
     1201 gcagggcctg cagtgcggcg cccttgaggt ggacttcgtt catctcgccg ggccggacgt
     1261 agcggtgctt ctgttcaaag agtccgtgtt cggcgaagac ccggcagcgc ttctcgatcg
     1321 ggtgcgtcac gcggaagtag acggtggcgt cgcagcatcc ggtgatgaac tgcggcgaga
     1381 agagccggac gttcttccct cccttgatgg cgatccgccg ctccgaactg cggagcgttc
     1441 cggcggagta cagggccgcg ttgcgccctg cggcgaaccc ttcctggctg acccagtcgg
     1501 cgaggtcgta cacgatgacg acgtttccgg cggcgaaaac cgaaggccgg gtcgtctgat
     1561 agaactcgtt gagaaccggc ccccccgtga cggggtgtat ttcgacgccc gccatgcggg
     1621 agagctcgtt ttcggggatg aggccgacgg cgagcagcat cgtgtcgcag gcgacgaaac
     1681 ggtcggttcc cggaatggga cgccggttct cgtcaacctt gacgacggtg attccttcaa
     1741 ggcggtcatg accgtgcacc ttgaccaccg agtgctccag atagaaggga ataccgtagt
     1801 cgtcgagaca ctgcgcgatg ttgcggcgca gaccgcccgc ccagggcatg atctcgtaga
     1861 ctccctcgac gctcgcgcct tcccagatca tgcggcgcgc catgatgagg ccgatatcgc
     1921 cggatccgag gataaccgct ttgtgccccg gcatgtaccc ctccatattg acgtagcgct
     1981 gcgcggtccc cgccgtgtag attcccgcgg ggcggctgcc cggaacgttg atcgctccga
     2041 gcggacgttc gcggcacccc atcgcaagga cggttgcctt cgggacgacg cggaagatcc
     2101 ccttctccgg gttcatcacc caaagactgc cgtcgggctc catcttgaag accatcgagt
     2161 tgacgaggaa ctgtaccccc gcctcgtggg ctctcttgat ccagcggtgc atatattcgg
     2221 ggccggtcag ctcttctttg aaggtatgca gtccgaagcc ggggtggatg
//
LOCUS       DAOPKU010000498         2258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_55743_length_2258_cov_4.160236, whole genome shotgun sequence.
ACCESSION   DAOPKU010000498 DAOPKU010000000
VERSION     DAOPKU010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55743_length_2258_cov_4.160236"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PK849_12760"
     CDS             <1..859
                     /locus_tag="PK849_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE67044.1"
                     /translation="LHGIGSAMVVPITMAIAADIAPKGEEGAIFGSLQAALFLGVGSG
                     PLLSGFLADTFGFYAPFYAMTALTLLALFLVLALFPSSPPKNTVKEEVGALKAFKGII
                     SDPEMLNVFIFQFSSAMCRGVLVMVIPLFASQFDLSLSRIGIIVSLNSISTGILQNVS
                     GKMADNVHKNTLIIIGGVISAVTLIGLPNLTTPLSLSVASVAFGVGHAFASPSLAAIA
                     AARGKNFGSGRTMGLFNIAFSIGMTIGPIMVGSLLDITGNGIPFYILSAFLAASALPF
                     FPVFKRGVC"
     gene            complement(908..1744)
                     /locus_tag="PK849_12765"
     CDS             complement(908..1744)
                     /locus_tag="PK849_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67045.1"
                     /translation="MKTKRFLFIFFLGFFLLRASSAFAWEVWTTTPWLALVSRFIGGV
                     FVAVRPIEFWSEDGQVVRKVQNRAIPKDSRIIALDTEEASRLGLNQKEWMSLALLYRT
                     APFDKKRLDYFFADPSSLPFIAQKVFTVLSHFDPANYPYYQRRLSEFQTRLDSTVIVG
                     RRLLSGSAFIYIGGDFSGMLTAAGCSMESRDESLNTLWEQGKELDLLFALLEDALKRR
                     IPVIVDTSSKKLKEALRNNKEILFLEKPALDQDILLFFHDQYIFLWNRLAALRQLRTP
                     QK"
     gene            complement(1741..>2258)
                     /locus_tag="PK849_12770"
     CDS             complement(1741..>2258)
                     /locus_tag="PK849_12770"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HPE67046.1"
                     /translation="LLFISEKQLDEGALSRRRAALVCGNSLRRWAEFIDIPTLLRTGK
                     GMSGGTVRSSIYEDAAEALFGAVFLDGGYKAADAVILRYLDFLMFLSSPEPADPKTEL
                     QILAQERGIGIPSYEILSVTGPSHQPLFHVRVLLGGSPAGEGRGTSKKAAEFNAAREG
                     YTFFTKETATP"
BASE COUNT          557 a          527 c          548 g          626 t
ORIGIN      
        1 gttgcacggg ataggttctg ctatggtagt gccgattacg atggcgatcg cggccgatat
       61 cgctccaaag ggcgaagaag gtgcaatttt cgggtccctt caggcggcct tgtttcttgg
      121 ggttggttcc ggtccgcttc tcagcggttt cttagccgac acttttgggt tctatgctcc
      181 gttttacgcc atgacagcac tcactctcct cgcccttttt ctcgttctgg ccctcttccc
      241 ctcttcccca ccgaaaaata cagtcaagga agaagtgggc gccttgaaag cttttaaggg
      301 catcatttct gatccggaga tgctcaacgt atttattttc cagttttctt cggcgatgtg
      361 ccgtggtgtt ctcgtaatgg ttattcctct ttttgcttcg cagtttgacc tttccttgtc
      421 gaggatcggc attattgttt cactgaattc tatttcgacc ggtattctac agaatgtatc
      481 cggtaaaatg gcggataatg tgcataaaaa taccctgatc attattggtg gagtgatttc
      541 agctgtgacg ctgataggac tccctaattt gacgacacct ctcagcctct ctgtagcaag
      601 cgtggctttc ggagtcgggc atgcgtttgc ttccccttcg cttgctgcga ttgcagcggc
      661 taggggtaaa aacttcggtt cagggaggac gatgggcctt tttaatattg cattcagcat
      721 aggcatgacg attggcccta taatggtcgg ctctttgttg gacattaccg gaaacggcat
      781 acctttttat atcctaagcg cttttctggc agcttctgcg cttccatttt ttccggtttt
      841 taaacgcggc gtgtgctaaa aattaaacaa agagacggga gaagtcctcc cgttcagaat
      901 ggttttttta tttctgcggg gtacggagtt gtctcagagc ggcaagtctg ttccacaaga
      961 aaatatactg atcgtgaaag aaaagcagta tatcctggtc aagggcgggt ttctccagga
     1021 aaagtatctc tttgttgttg cgtagcgctt ctttaagctt cttggaagat gtatcgacga
     1081 ttactggaat tctccttttg agagcatcct caaggagagc gaaaagaagg tcgagttctt
     1141 ttccttgctc ccaaagtgta ttcaatgact catcccttga ttccatcgaa catccagctg
     1201 cagtcagcat cccggaaaaa tcacccccaa tatagataaa ggctgatccg ctcaaaagcc
     1261 ttcttcccac gatgaccgtg ctgtcgagac gcgtctgaaa ttctgaaagc cgacgttggt
     1321 agtaggggta gtttgccggg tcgaaatgtg agaggaccgt gaaaaccttc tgtgctatga
     1381 atggaagcga agacgggtca gcaaagaaat aatccaaacg ttttttgtca aaaggagctg
     1441 tacggtaaag aagtgcaagc gacatccact ctttttggtt caatccaagc cgggaagctt
     1501 cttcagtatc aagcgcaatg atacggctgt ctttgggtat tgcacggttt tgtactttgc
     1561 gtacaacttg accgtcttca gaccaaaact caatcggacg aaccgctaca aaaacgcctc
     1621 cgataaagcg gcttacgaga gcaagccaag gcgtcgtcgt ccatacttcc caagcgaagg
     1681 cggaggaagc gcgaagaagg aaaaatccaa gaaagaaaat gaaaagaaat cgtttcgttt
     1741 tcatggcgtg gctgtctcct tcgtgaagaa agtataccct tctcttgccg cattgaattc
     1801 tgccgctttt ttcgatgttc ctcttccttc tccagccggg gaacctccaa ggagcactcg
     1861 cacatgaaat agaggttgat gagaaggacc ggttacggaa agaatttcgt aggaaggaat
     1921 gccgatgcct ctctcttgag cgagaatctg aagctcggtt ttcgggtcgg caggttccgg
     1981 agaagaaaga aacattagaa aatcaagata tcttagtata acagcatcag cggctttata
     2041 tcctccatcg agaaaaactg ctccgaaaag ggcttcagcg gcatcttcgt agattgaact
     2101 gcggacagtg ccgccagaca ttcctttccc tgtcctcaga agcgttggaa tatcaataaa
     2161 ttccgcccat cggcgaagag agtttccgca aacaagtgcg gctctcctcc gtgaaagagc
     2221 tccttcatca agctgttttt ccgagataaa aagcaaat
//
LOCUS       DAOPKU010000499         2256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_55818_length_2256_cov_4.527942, whole genome shotgun sequence.
ACCESSION   DAOPKU010000499 DAOPKU010000000
VERSION     DAOPKU010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55818_length_2256_cov_4.527942"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PK849_12775"
     CDS             <1..280
                     /locus_tag="PK849_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE67047.1"
                     /translation="ARSVVKLLTVASEWSNANQTEAGRIAAQWIGIPEEATEHATIVY
                     GTDPTESWLKGAGVYLDVLNGIGSFKGSLKGKTLEEAIPLVFDFSFNK"
     gene            378..1211
                     /locus_tag="PK849_12780"
     CDS             378..1211
                     /locus_tag="PK849_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE67048.1"
                     /translation="MRHLKSLVLSLLVPALFLCLWAYMAKRTANDVVLPPIGQVATHF
                     ASPGTSLIGMGSLVSNILISLLRVLAGYLIAVLLAIPAGLLLGHNRLLSELFTPFLGL
                     FRPIPPLAWVPLVLAWFGIASFATLFGVEEGEWYVFLNNLKFSMVFIIFIGAFYPILS
                     GTMHGVSGVRSILVDSARVLGASDRDIFLKVLLPASLPSIVNGLRIGLGVAWMCLVSA
                     EMLPGSISGVGYLITHAYTVARTDIVIVGMIAIGLLGAAMDRGFLFVEERAFQWQRLT
                     R"
     gene            1190..2014
                     /locus_tag="PK849_12785"
     CDS             1190..2014
                     /locus_tag="PK849_12785"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE67049.1"
                     /translation="MAAPHEVTAMRDDAVIRAAHIGKTFASRSGKELVALDDVSLAVT
                     EGEFVCLLGPSGCGKSTLLRILAGLETHFEGSVFADGEKVAGPSRKRGMVFQEYSLFP
                     WRNIEENVAAGLEFAGVPADERRERSLRHLELVGLSGFARALPHELSGGMRQRAAIAR
                     ALAADPHILLMDEPFGALDAHTRILLQKELLRIWSASRKTVIFVTHSVDEAVYLADRI
                     VVMSARPGRIVEEIEVDMERPRRRDDPRWGVLAARILALLDEATADGGLSEDEEVR"
BASE COUNT          369 a          746 c          734 g          407 t
ORIGIN      
        1 cgcccgaagc gtcgtgaagc tgctgaccgt cgcctccgaa tggagcaacg cgaatcagac
       61 ggaggcgggg cgcatcgccg cgcagtggat cggtatcccg gaggaggcga ccgagcacgc
      121 caccatcgtc tacggaaccg accccaccga atcctggctc aagggcgccg gagtctacct
      181 cgacgtcttg aacggcatcg gcagcttcaa aggctccctg aaaggcaaga cgctcgaaga
      241 ggcgattccg ttggtcttcg acttctcctt caacaagtaa acggaagacg attcacggcc
      301 tccgcgcacg ggggtccctc gtccatcggc ggaggggccc ccgacgcgcg cgggggcgga
      361 aaggatatgc gaccgatatg cggcatctga aatctcttgt tctctcgctt cttgttccag
      421 cgctcttcct gtgcctgtgg gcctacatgg cgaagcgcac ggcaaacgac gtcgtcctcc
      481 cccccatagg gcaggtggcg acgcacttcg cgtctccggg gacgagcctg atcggaatgg
      541 gctctctcgt ctccaacatt ctcatcagcc tgctccgggt gctcgcaggc tacttgatcg
      601 ccgttctcct cgcgatcccc gcggggctgc tgctggggca caatcgcctt ctctccgaac
      661 tcttcacccc cttcctcgga ctcttccgcc cgataccgcc cctcgcctgg gttccgctcg
      721 tgctcgcgtg gttcggcatc gccagcttcg ccacgctctt cggcgtggag gagggggagt
      781 ggtacgtctt cctgaacaac ctgaagttct cgatggtctt catcatcttc atcggcgcat
      841 tctaccccat cctctccggg acgatgcacg gcgtctccgg cgtgcgctcc atcctcgtcg
      901 actccgcgcg ggtgctggga gcctccgacc gcgacatctt cctcaaggtg ctgctgccgg
      961 cgtctcttcc ctccatcgtg aacgggcttc gcatcgggct gggcgtggcg tggatgtgcc
     1021 tcgtctcggc ggagatgctc cccggaagca tctccggcgt gggctacctg atcacgcacg
     1081 cctacaccgt cgcccgcacc gacatcgtta tcgtcggcat gatcgccatc ggactgctcg
     1141 gcgccgcgat ggaccgcgga ttcctcttcg tcgaggagcg cgccttccag tggcagcgcc
     1201 tcacgaggtg acggcgatgc gcgacgacgc ggtgattcgg gcggcgcata tcggcaagac
     1261 cttcgcctcc cgctcgggaa aggagctggt cgcgctcgac gacgtctctc tcgccgtgac
     1321 cgagggggag ttcgtctgcc ttctgggccc ctccggctgc ggcaagtcca ccctgctgcg
     1381 catcctcgcg gggctggaga cgcacttcga gggaagcgtc ttcgccgacg gagagaaggt
     1441 cgcggggccg agccgcaagc gaggcatggt ctttcaggag tactcgctct tcccgtggcg
     1501 gaacatcgag gagaacgtgg cggcggggct tgagttcgcc ggcgtcccgg cggacgagcg
     1561 cagagaacgg agccttcgcc atctggagct cgtcgggctt tcggggttcg cgcgcgcgct
     1621 cccgcacgag ctttcgggcg gcatgcgcca gcgggcggcg atcgcccgcg ccctggccgc
     1681 cgatccgcac attctgctga tggacgagcc gttcggggct ctggacgccc acacgcgcat
     1741 cctgctgcag aaggagctgc tgcggatttg gagcgcctcg cgcaagacgg tgatcttcgt
     1801 tacccacagc gtggacgagg cggtctacct ggccgaccgg atcgtcgtca tgtccgcccg
     1861 cccggggaga atcgtcgagg aaatcgaggt ggacatggag cgtccgcgcc gacgcgacga
     1921 cccgcgctgg ggcgtcctgg cggcccgtat cctcgccctg ctcgacgaag cgacggcgga
     1981 cggagggctt tccgaggacg aggaggtaag gtgaagtctc ccgccgaagt ataaccccga
     2041 cgctgcctcg acgctccgga gcggggcgcg cgcggttcct ccggaaagcc gagggagggc
     2101 gcagcttttt caatccacaa tacgtaagga gaggatagta aatgggaaca atcgatcaga
     2161 tcgtgcagca cgtccgggag catatggccg acggaccggt cgcggtgaaa gaggcgcatg
     2221 aagccggaac gaaggtgata ggaacatact gcacct
//
LOCUS       DAOPKU010000500         2252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_55958_length_2252_cov_4.859809, whole genome shotgun sequence.
ACCESSION   DAOPKU010000500 DAOPKU010000000
VERSION     DAOPKU010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55958_length_2252_cov_4.859809"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>2252
                     /locus_tag="PK849_12790"
     CDS             23..>2252
                     /locus_tag="PK849_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE67050.1"
                     /translation="MIPKEPTYSDLQQRIAGLEEALRMKSLAEETMREQETRYRAIVD
                     QATSDALLVHDFQGRFLEVNRRACESLGYSKEELLRMSVTDIECDFDLKSAQAEWSRI
                     EPGIPFILYGHQRRKDGTVFPVEIHFGCSFWNGETFYLGLVRDITKRKLTEEALRESE
                     ERFRNMVEGAPDAIYIQIDRKFAYVNPAACRLFGVASAEELLGMPLLERIHPLYHDVA
                     RERMRRLSDERSPQRERIELRFLRMDGSEVWVETSGEPINYQGKYGALGFARDITWRK
                     EAEEALGESEERFRSLFRDSLSVMFLLDPVTGRFLDVNRECGNFYGWPAQELLNMNIM
                     DIHTPGEEALCEIEKAASGEQNRFESRHRLADGSVRDVEVFTSKICVRGECLLYCIVN
                     DITAKKAAQRQVRLLGRAVEQNSESVIITDAAGNIEYVNPAFTQSTGYAAEDVAGRNP
                     RVLKSGEQSEAFYKRMWETIISGETWRGEMHNKRKNGELFWENVAISPILDENGAIAH
                     FIAIKQDITERKKLIESLVRAKEKAEESDRLKTAFLQNVSHEIRTPMNGILGFLHLLG
                     QPALDDDERSEYIRIVNQSGQRLLSTINDIIEASRIEAGKTTVQMDDVDVEQTMAFHF
                     AFFRPRAEEKGLFLTLSEHLRGEEALLRTDKHKLDSILNVLLGNALKFTCSGGVEFGN
                     RLEGSELLFFVKDSGEGIPEDRLEAVFDSFVQADLGVARSHEGAGLGLTIAKANVEAL
                     GGK"
BASE COUNT          545 a          551 c          713 g          443 t
ORIGIN      
        1 ctgaaagaga gatgagggaa ggatgatacc gaaagaaccg acgtacagtg atcttcagca
       61 gcgtatcgcc ggtcttgaag aggcgttgcg gatgaagagt cttgcggagg agacgatgcg
      121 cgagcaggaa acgcgatatc gcgccattgt cgaccaggcg acgagcgacg ccctgctggt
      181 ccatgatttt cagggccggt tcctggaagt caaccggcgt gcgtgcgaaa gccttggcta
      241 ttcaaaagaa gaacttcttc gaatgtcggt gaccgatata gagtgcgact ttgatctgaa
      301 gagcgcgcag gcagagtggt cgagaatcga gccgggaatc cccttcattc tgtatggaca
      361 tcaacggcgc aaagacggta cggtttttcc ggttgaaatt cattttggct gttctttctg
      421 gaacggggag acgttctatc ttggcctggt gcgcgatatc acaaagcgga aactgacaga
      481 agaagccctg cgcgagagcg aagagcgttt ccggaatatg gttgaaggcg cgcccgacgc
      541 gatttatatc cagatcgaca ggaagttcgc ctatgtaaat cccgccgcgt gccgtctttt
      601 cggcgtcgct tccgcggagg aactcctcgg catgccgctt ctggagcgta ttcatcctct
      661 ctaccatgac gttgcccggg agaggatgcg gcgtctgagc gatgagcgca gcccgcagcg
      721 tgaacgtata gagcttcgtt ttttgcggat ggacgggagc gaagtgtggg tagaaacaag
      781 cggagagcct atcaactatc agggaaaata cggagctctc ggtttcgccc gcgatattac
      841 atggcggaaa gaggcggaag aagcgctcgg agagagcgaa gaacgtttcc gcagtctttt
      901 ccgggacagc ctttccgtga tgtttttgct cgaccctgtt acgggacgct ttcttgacgt
      961 caacagagag tgcgggaact tctacgggtg gccggcgcag gagcttctga acatgaacat
     1021 aatggatatc catacgccgg gcgaagaagc gctgtgcgag atagaaaaag ccgcgtcggg
     1081 agagcagaac cgtttcgaat ccaggcatcg tctggcggac gggtccgtgc gcgacgtgga
     1141 agtgttcacc agcaagattt gcgtgcgggg cgagtgcctg ctctactgca tcgtcaatga
     1201 catcacggcg aaaaaagcgg cgcagcgcca ggtacgtctg ctcggcagag cggttgagca
     1261 gaattcggag agcgtcatta ttaccgatgc cgcagggaat atagagtatg tcaatccggc
     1321 gttcacccag tccacagggt atgccgcgga ggatgtcgca ggaaggaacc ctcgcgtgct
     1381 caagtccgga gagcaaagcg aggcttttta caaacgcatg tgggaaacga tcatctccgg
     1441 ggagacgtgg agaggggaga tgcacaacaa acgaaagaac ggcgaactct tctgggagaa
     1501 cgtcgcgatc tcgcccattc tggatgaaaa cggcgctatt gcacatttta tcgcgatcaa
     1561 gcaggatatc accgagagaa agaagctgat cgagtccctt gtacgcgcca aggaaaaggc
     1621 cgaagagagc gaccggctga agacggcgtt cctccagaac gtatcgcacg agatacggac
     1681 gccgatgaac ggaattctcg ggtttcttca cctgctcggg cagccggctt tggacgacga
     1741 cgagcgaagc gagtacatcc ggatcgtcaa ccagagcgga cagaggctcc tgagcacgat
     1801 caacgatatt atagaagcgt ccagaatcga agcgggaaag acgacggtgc agatggacga
     1861 tgtcgacgtt gaacagacga tggcgttcca tttcgctttc ttccggcccc gggcggagga
     1921 gaaggggctc tttttgacgc tttcggagca tcttcgggga gaagaggcgt tgcttcgcac
     1981 cgacaaacac aagctggaca gcatcttgaa cgttctgttg ggcaacgcgc tcaaattcac
     2041 ctgcagcgga ggcgtcgaat tcgggaatcg cctggaaggc tccgaactgc tctttttcgt
     2101 gaaggacagc ggagagggaa tccccgagga tcgtctcgaa gccgtcttcg acagctttgt
     2161 gcaggcggat ctgggggtgg cgcgttcgca tgaaggcgcc ggattgggac tgaccatagc
     2221 aaaggccaac gtcgaagccc tcggcgggaa aa
//
LOCUS       DAOPKU010000501         2249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_56060_length_2249_cov_3.722881, whole genome shotgun sequence.
ACCESSION   DAOPKU010000501 DAOPKU010000000
VERSION     DAOPKU010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56060_length_2249_cov_3.722881"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..962
                     /gene="prxU"
                     /locus_tag="PK849_12795"
     CDS             291..962
                     /gene="prxU"
                     /locus_tag="PK849_12795"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048998.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:483..485,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HPE67051.1"
                     /translation="MGAEAAPAEEVKKEAVRMGVTVGMKAPDFTAPAFYKGGFTTVKL
                     SQFAGKWVSLCFYPGDFTFVUATEVSAVADRYAEFQALGVEVISMSVDSQFVHKVWND
                     VELSKMIEAGGVPFHMGSDGGGAIGTVYGVYSPAQGVDFRGRFLIDPDGVIQAMEILT
                     PPVGRNVDETLRQVQAFQLVRKTGGKEVTPSGWTPGGKVLNPGPDLVANVWKVWNPKD
                     VYGKK"
     gene            complement(1026..>2249)
                     /locus_tag="PK849_12800"
     CDS             complement(1026..>2249)
                     /locus_tag="PK849_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67052.1"
                     /translation="TPKKEPMKAEAQTPAKGTTYTGEILPPENDDEKNSASAAEETDK
                     AEKETSKETPETEQPPVKEENTEKDDDADTDADTEEEKEEENDEVVDTNDEVITRPSP
                     SPEPPVVTEDPDDIIPPELIRPSVLLPEMTDTEREDIARIVAAKRHALDLGDARYAAL
                     SAFLPSGGNSLQMKAISDDVQTAIIAHIAPAMHRDAEGNDALPFLLQTESFELETGAT
                     NLRGKFLVVRITFTVTHSDLASADPKIVSRIEKGVASGRSLGDAFLDELRIFKWIGEG
                     DNARCFDLAESVRAGGYSLNSFFEAQAVKNPAEEIFDTKAQDASYTVAFTLLIFDGVT
                     NDLSRLVQPLEGAGIIVFDGLKDGKYADPLILAAPRPKNAQTQGTSGCATSAGGISPA
                     LLLLPGALLLLRRRK"
BASE COUNT          435 a          595 c          655 g          564 t
ORIGIN      
        1 tatcttgctc ctattcttgc atcctcggag aaaaagtgca aagcatttct tgcgtccact
       61 ggtatgcggt gtaaaaaaga caataaaaaa ccttgacttt taaatgactt cccatatact
      121 aataagcaaa taaaagaggc gtgcgtgttt caagaatgtt tttttgccgg aagcttgttt
      181 ttatctcttg ttgcgtctct tgtaatgttt tgcttcccgg tatgaaagga ggagttctca
      241 tgagcgagga aaaggtagga tgtacggttc ccgctgcgga ggtcgaagga atgggcgccg
      301 aggctgcgcc cgccgaagag gtgaagaagg aggctgttcg aatgggtgtt acggttggca
      361 tgaaggcgcc tgatttcact gcgccggcgt tttacaaggg cggattcacc acggtgaagc
      421 tgtcgcagtt tgcgggaaag tgggtttccc tctgcttcta tccgggcgac ttcaccttcg
      481 tctgagcgac tgaagtgtcg gcagttgccg accgttatgc ggagtttcag gcattgggcg
      541 tggaggttat ctccatgagc gtggacagtc agttcgtcca caaggtatgg aacgacgtcg
      601 agctctccaa gatgatcgag gcgggcggag ttcccttcca catgggctcc gacggcggcg
      661 gcgccatcgg aaccgtctac ggcgtgtaca gccccgctca gggcgtcgat ttcagagggc
      721 gcttccttat cgacccggac ggcgtgatcc aggcgatgga aattctcact cctcccgtgg
      781 gcaggaacgt cgacgagacg ctgcgccagg ttcaggcgtt ccagcttgtg cgtaagaccg
      841 gcggcaaaga ggttactccc tccggatgga cgccgggcgg caaggtgctc aaccccggtc
      901 ccgacctggt ggcgaacgtc tggaaagtgt ggaatcccaa ggacgtctac ggcaagaagt
      961 aagccgtttg gaatagagcg acaaaaaagc gccccttcat caagaagggg cgctttttca
     1021 gctgcttatt ttcttcgccg cagcaggaga agagctcccg gaaggagcag gagggccggc
     1081 gagattcctc ccgcggatgt agcgcatccg cttgttccct gtgtctgcgc gttcttcggg
     1141 cgcggcgccg cgagaatgag cggatcggcg tatttcccgt ccttcaggcc gtcgaacacg
     1201 ataatgccgg cgccctcaag cggttggacc agtctcgaga ggtcgttcgt gacgccgtcg
     1261 aagatgagga gcgtgaacgc gaccgtatag gaggcgtcct gtgccttcgt gtcgaagatt
     1321 tcttcggcgg ggttcttcac ggcttgtgcc tcgaagaagc tgttcagaga gtagcctcca
     1381 gcccgaacgc tctccgccaa atcaaagcag cgcgcgttgt ccccttcccc tatccatttg
     1441 aagatgcgga gttcgtcaag aaacgcgtcg cccaatgatc tccctgaagc gacgcccttt
     1501 tcgatcctgc tcacgatctt cggatcggcc gaggcaaggt cggagtgggt cactgtaaag
     1561 gtaatcctca cgacgaggaa cttccccctg aggttcgtag ctccggtctc aagttcgaag
     1621 ctctcggtct gcagcaggaa ggggagcgcg tcgttcccct ccgcgtctct atgcatcgca
     1681 ggagcaatat gtgcaatgat tgcggtctgc acatcatccg agatagcctt catttgtaag
     1741 gagtttccac cggaggggag aaacgccgat aaagcggcgt aacgcgcatc tccaaggtca
     1801 agagcatgtc tctttgccgc gacgatacgg gcgatgtcct ccctttctgt atcggtcatc
     1861 tcgggaagaa gcaccgacgg cctgatcaac tcagggggaa tgatgtcatc cggatcttcc
     1921 gtaacaacag gcggttcggg actcggcgaa ggtctcgtaa tcacctcgtc gtttgtatca
     1981 acgacctcgt cattttcttc ttctttttct tcttcggtat cggcgtcggt gtccgcatcg
     2041 tcatctttct ctgtgttctc ctccttcact ggaggctgtt cggtttcggg agtttctttc
     2101 gacgtttctt tttcagcttt gtctgtctct tcggccgccg aagcggagtt tttttcgtca
     2161 tcattttccg gcggcagtat ttcgcccgta tacgttgtgc ccttcgccgg tgtttgtgct
     2221 tccgctttca tgggttcttt tttgggtgt
//
LOCUS       DAOPKU010000502         2248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_56098_length_2248_cov_3.611035, whole genome shotgun sequence.
ACCESSION   DAOPKU010000502 DAOPKU010000000
VERSION     DAOPKU010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56098_length_2248_cov_3.611035"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK849_12805"
     CDS             complement(<1..342)
                     /locus_tag="PK849_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67053.1"
                     /translation="MRNNVGEEIVHRLAVSGQEFSLYIHLPFCARKCPYCSFFSVVPR
                     NGEIEEYCRLLHSEIRFFGERLAGSGAPAGTIYFGGGTPSLLAPADWERLFKCLRKHI
                     LFDQNVEITVEA"
     gene            complement(332..2140)
                     /gene="lepA"
                     /locus_tag="PK849_12810"
     CDS             complement(332..2140)
                     /gene="lepA"
                     /locus_tag="PK849_12810"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPE67054.1"
                     /translation="MEQKRIRNFCIIAHIDHGKSTLADRLLELTQTVESRDMKHQMLD
                     TLDLERERGITIKLVPVRMKYRAEDGEDYILNLIDTPGHVDFGYEVSRSLAACEGALL
                     VVDATQGVEAQTLANAYMAVDQGLEILPVINKIDLPSSRPDYVIKEIEDVVGLDCDKV
                     ILASAKEGKGVPEILERIVHGVPAPSGNPEAPLQAMIFDSKYDNYKGVICYIRVMNGT
                     LRPGRNIRFMATGGVFPLEEVGVLRPNFEPCDELTAGEVGYIVSNIKTVAEAHVGDTI
                     TEDARPATAALPGYRKVKPVVFCGFYPVERDDYPQLRDALEKLTLNDSSISFEPETSL
                     ALGFGFRCGFLGLLHMDVSKERLRREYSVELVATSPNVIYELTTKSGEIIEAHRPSDF
                     PQIGDIEEVREPMIKLTLFVPSDYVGKVMKLCQDKRGTYISMDYLAPDRVRLIYEVPL
                     AEFIIDFHDKLKSHTRGYASLDYEHTGLKASDLVRVDVLINGEAADAFSFICHREAAY
                     NRGQAVVRKLKELIPSQLFEVPLQASIGKKVIVRQNVKPLRKDVLAKCYGGDITRKRK
                     LLEKQKEGKKRMKQIGRVSIPQEAFLAFLKVEEDEE"
BASE COUNT          517 a          620 c          569 g          542 t
ORIGIN      
        1 tgcctccacg gtgatctcta cattttgatc gaaaaggata tgtttgcgta aacatttgaa
       61 aagccgctcc caatcagccg gagcaagcag cgacggggtg ccacctccga agtaaatagt
      121 ccccgcgggt gctccgcttc ccgcaagacg ctctccgaaa aaacggatct ccgaatgcag
      181 aagcctgcag tactcttcta tttccccgtt tcgcggaacc acgctgaaaa acgaacagta
      241 gggacatttc cttgcacaga acggcagatg aatataaaga gaaaactcct gtccggagac
      301 ggcgagccgg tgaacaatct cctcgccgac gttattcctc atcctcttct accttcagaa
      361 atgcgagaaa agcttcctgg gggatagaca cgcgcccaat ctgcttcatg cgcttctttc
      421 cctctttctg cttttcaagg agtttccgtt ttcgggtaat gtcgcctccg tagcacttcg
      481 caagcacgtc ttttcgaagc ggctttacat tctgtcgaac aatgactttt tttcctatgg
      541 acgcctgaag aggcacttca aaaagttggc tcggaatcag ttccttgagt tttcgcacca
      601 cagcctggcc ccgattgtag gcggcttccc tgtggcagat gaaggagaac gcatcggcgg
      661 cttctccgtt gatcagcaca tcgacacgaa ccaggtcgga cgccttcaga ccggtatgct
      721 cgtagtcaag ggacgcatac ccccttgtat gggacttcag cttgtcgtgg aaatcaatga
      781 taaactccgc caacggcact tcatagatca agcgtacacg gtccggggca agatagtcca
      841 tggagatata cgttccccgt ttatcctgac agagcttcat cacctttcca acgtagtccg
      901 acggaacgaa gagagtcagt ttgatcatcg gctcccgcac ttcttcgata tcaccgatct
      961 gaggaaaatc ggagggacgg tgggcttcaa tgatctcgcc gctcttcgtc gtcaattcat
     1021 atatgacgtt cggtgaagtg gcgacgagct ccacgctgta ctcgcggcga agacgttcct
     1081 ttgacacatc catgtggaga aggccgagaa acccgcaacg gaacccgaat ccgagagcaa
     1141 gcgaagtctc cggttcaaag gagatcgagg agtcgttaag cgtcagcttt tcaagagcgt
     1201 cacgaagctg agggtagtcg tcgcgttcca cgggatagaa gccgcaaaaa acgacaggtt
     1261 tgacctttct gtagccgggg agcgccgccg tcgccggacg cgcatcctcc gtgatggtgt
     1321 ctcccacgtg cgcttctgcc acggtcttga tgttcgatac gatatagcca acctctcccg
     1381 ccgtcagttc gtcgcacggt tcaaagttcg ggcgcagcac gccgacttcc tccaggggaa
     1441 aaacgccgcc ggtcgccata aaccggatat tgcggccggg gcgcagcgtt ccattcataa
     1501 cgcgtatgta acagataacg cctttgtaat tatcgtactt tgagtcgaag atcatcgcct
     1561 gcaacggcgc ttcaggattg cccgacggcg cgggtactcc gtgaacgatg cgctccagta
     1621 tctcgggaac tcccttaccc tccttggcgc ttgcgagtat caccttgtcg cagtcaagcc
     1681 cgacaacatc ttctatttcc ttaattacat agtcggggcg ggatgacgga aggtcgatct
     1741 tgttgatcac cgggagaatc tccaaccctt gatcaaccgc catatacgcg ttggcgagag
     1801 tctgcgcctc aacgccctgt gtggcgtcga caacgagcag cgctccttca caggcggcaa
     1861 gagagcggga gacctcatat ccgaagtcca catgccccgg agtatcgatc aggttgagaa
     1921 tatagtcttc tccgtcttcg gctcgatatt tcatgcgtac cggaacaagc ttgatggtga
     1981 tcccccgttc tctttcaaga tcgagggtat caagcatctg gtgtttcatg tcccgtgatt
     2041 caacggtttg cgtcaattcc agcagtctgt cggcaagggt agattttcca tgatcaatat
     2101 gggcgatgat gcagaagttg cgaatgcgtt tctgttccat acaacgtctc cttccaaaaa
     2161 tggcggtgct tcagtctcga tactggctcc tttaaaggaa gcctgtcatt ttttccagat
     2221 cagatttgtt gttgaggttt tcaaaaga
//
LOCUS       DAOPKU010000503         2238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_56433_length_2238_cov_4.212093, whole genome shotgun sequence.
ACCESSION   DAOPKU010000503 DAOPKU010000000
VERSION     DAOPKU010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56433_length_2238_cov_4.212093"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /gene="purH"
                     /locus_tag="PK849_12815"
     CDS             <1..1500
                     /gene="purH"
                     /locus_tag="PK849_12815"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPE67055.1"
                     /translation="YDKTGITELAKTLTDLGWELLSSSGTAEHLRKAGLLVTEVADVT
                     GYPHILGGRVKTLHPKVFGGILARRENGGDLKDVEEHDLPLIDMIVCNLYPFEQAAKK
                     HLPLDDLLENIDIGGVSLLRAAAKNYRQVVALMEPEDYPTVAEELRSEGDVTLPTREQ
                     LAIKAFAGTASYDGMIVSGLKETTGYASPALPAKIPLILQKQQTLRYGENPHQEAALY
                     LPALADLPWEQLSGKPLSYNNILDADCAMRGCALLQNCCGALVIKHTTPCGMACGETP
                     FEAYERAYGCDPVSAYGGVVGISRKVDMETVLALADRFTDVLVAPEYDEETVALLAEK
                     KPALRVLKWKGGRVLTDQITGTWSGLLVQADSLPPVPELEKGEWVGAPRPDLWDDLLL
                     AWKAAAISKSNAIALVKNGEAIGIGRGCCSRLHAVDFAVRQAGQKAKGSVLGSDAFFP
                     FTDGVELAADAGVAAIIQPGGSVRDKDVCAAVERRGISMFITGWRTFRH"
     gene            1520..>2238
                     /locus_tag="PK849_12820"
     CDS             1520..>2238
                     /locus_tag="PK849_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPE67056.1"
                     /translation="MTGKKNILVIGSGAREHALVHTLSRSGTVREIHAAPGNPGMLPL
                     AKLHPVSDAACASILPLVKRHGIDLVVVGPEAPLVAGLADELRSAGVEVFGPGRAGAL
                     LEGSKVHAKKFMDRWGVPTAPWDECTSLQDVEEALAKRSAPFIVKADGLAAGKGVFVT
                     KTREEAFLAAENLLVRGALGEAGKRIIIEDALSGEELTVLAVSDGATYRILPPSQDHK
                     RVYEEDKGPNTGGMGAYSPVP"
BASE COUNT          495 a          633 c          676 g          434 t
ORIGIN      
        1 tatgacaaga cggggataac agaactggcg aagacgctga ccgacctggg ctgggaactg
       61 ctctccagtt cggggactgc ggaacacctg cggaaagcag ggcttctggt aacggaagtt
      121 gctgatgtga cggggtatcc ccacatcttg ggaggtcggg taaaaaccct ccaccccaaa
      181 gttttcggag gcattcttgc ccggagagag aacggcggcg atctgaagga tgttgaggaa
      241 cacgatcttc cccttatcga catgattgtc tgcaacctct accctttcga gcaggcggcc
      301 aagaaacacc ttccgctcga cgacctgttg gagaatatcg atatcggggg agtttccctg
      361 ctgcgcgctg ccgcgaagaa ctaccggcag gtcgtcgcgc ttatggaacc cgaggactac
      421 ccgaccgtcg ctgaagaact gcggtcggaa ggagatgtaa cgctgccgac acgagaacag
      481 cttgcgataa aagcctttgc aggaaccgca tcctatgacg gaatgatcgt ttccggtctg
      541 aaggagacga cagggtacgc ctctccggct ctccctgcca aaataccgct catactccag
      601 aaacaacaga cgctccgata tggagagaat ccgcatcagg aagcggcgct ctatcttccc
      661 gcactcgccg atcttccctg ggagcagctc tccggcaaac ctctttcgta caacaatatt
      721 ctggacgccg actgcgcgat gcgcggatgc gcgcttctcc agaactgctg cggagctctg
      781 gtgatcaagc atacaacgcc gtgcggcatg gcctgcgggg aaactccttt tgaagcgtat
      841 gaacgcgcct acggctgcga tcctgtctcc gcatacggcg gagtcgtcgg catctccaga
      901 aaagtcgaca tggagaccgt tcttgccctg gcagaccggt ttacggacgt gctcgtcgct
      961 ccggagtacg atgaggagac cgtcgctctg cttgcggaga aaaaaccagc ccttcgcgtg
     1021 ctcaaatgga agggaggcag agtgcttacc gaccagatta ccggaacgtg gagcgggctg
     1081 cttgtccagg cagattctct gccgcctgtt ccggaactcg aaaagggcga atgggtgggc
     1141 gcaccccgtc cagatctttg ggatgacctg cttctggcgt ggaaggccgc cgcgatttca
     1201 aagagcaacg ccatcgctct cgtcaaaaac ggcgaagcca tcggcattgg cagagggtgc
     1261 tgcagcaggc tgcacgcggt tgatttcgcg gttcgtcaag caggtcagaa ggcgaaggga
     1321 tccgtgctcg gttccgatgc attcttccca tttaccgacg gagtcgaact cgccgcagat
     1381 gcgggtgtcg ccgcaatcat ccaacccggc gggtccgtga gggacaaaga cgtatgcgcc
     1441 gcagtcgaac ggcggggcat ctccatgttc attaccggat ggcgtacttt ccgccactga
     1501 tgaaagagac gatatcgcta tgacgggaaa gaagaatatc ctcgttatcg gaagcggcgc
     1561 acgggagcat gctctcgtgc atacgctttc ccgctccgga accgtccggg agatccatgc
     1621 ggctccgggg aaccccggta tgctgccttt ggcaaaactc caccccgtca gtgatgcagc
     1681 atgcgcatcg atacttcctc ttgtgaagcg gcacgggatt gatcttgtgg tcgtcgggcc
     1741 ggaggcgcct ctcgtcgccg gtctggccga cgaactccgc tcggccggcg tcgaggtctt
     1801 cggcccgggg cgtgcgggcg cgcttcttga agggagcaaa gtccacgcga aaaaattcat
     1861 ggacagatgg ggagttccga ccgctccctg ggatgaatgc acttcgcttc aagacgttga
     1921 agaagcgctg gccaaacgaa gcgccccttt tattgtcaag gccgatggtc ttgccgcagg
     1981 aaaaggcgta tttgtaacaa aaacgcgtga ggaagcgttc ttggcggcgg agaacctgct
     2041 tgtacgaggc gcgctcggcg aggcgggcaa acgcattatc attgaagacg cactctcagg
     2101 agaggaactc acagttttgg ccgtgagcga cggggcaacc tacaggattc tgcctccgag
     2161 ccaggaccat aagcgcgtct acgaagaaga caaaggaccg aatacaggcg gtatgggtgc
     2221 atactcaccc gttccctg
//
LOCUS       DAOPKU010000504         2233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_56609_length_2233_cov_3.992195, whole genome shotgun sequence.
ACCESSION   DAOPKU010000504 DAOPKU010000000
VERSION     DAOPKU010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56609_length_2233_cov_3.992195"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..395
                     /locus_tag="PK849_12825"
                     /pseudo
     CDS             1..395
                     /locus_tag="PK849_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
     gene            420..803
                     /locus_tag="PK849_12830"
     CDS             420..803
                     /locus_tag="PK849_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPE67057.1"
                     /translation="MGNRLQFAVFREALHILEEGIAGVDDVDRAMRYGPGFRYPVIGP
                     LQTADLGGLDTFYYISSYLFASLSDMKEPSVTLKNLMETQQLGVKTKSGFYDYSNGKD
                     EETIRRRDKMFFKMLKHIHSNGKNS"
     gene            841..1737
                     /locus_tag="PK849_12835"
     CDS             841..1737
                     /locus_tag="PK849_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE67058.1"
                     /translation="MKGKENKKDESGWSEKQRDSLDGRLIDHYIYVLSRLSGMQVADV
                     GVCICDLDGVVFYKPARSLDLKVKHGDEIRPGSLLYRALHERRRVTAKMDSSLYGVPY
                     VGVGTPIMDEGGQVLGAVAITESTLNYENLKESSMKIAESIEMLAGASEEIAAQSQEM
                     SGTAAIMKETLERSRESVKASDQVIGLIKMIATRTNLLGLNASIEAARVGEQGRGFNV
                     VAEEIRKLAGVTGDSIQNIAHTLASIQSDNAENCRQMLVMSSIITQVSDATTQIAETA
                     QALNEMASSLNEMAEKIMESIN"
     gene            complement(1750..>2233)
                     /locus_tag="PK849_12840"
     CDS             complement(1750..>2233)
                     /locus_tag="PK849_12840"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HPE67059.1"
                     /translation="FVMLPVRNASKLTSRIVTTSICHVLVNKEHRFATRNTPIHTSEL
                     VEERIITFSTTATLHDAFIRHCENEGFEPNIVYKTLMPNFIFEMVHLGLCIGILPYPV
                     VKQFIFDGLVSIPLEPKIIWEIAVISKKDNYLSFAARNFLSFVEEYSVRKGKEWQGLE
                     "
BASE COUNT          603 a          471 c          624 g          535 t
ORIGIN      
        1 tcaatgccaa tcttgtcgaa gaggggctct taacggagga tgcaacgaaa ggcattgtcg
       61 ataacatctc gtactcgacc gacaaaaagg tatttttcga cgccgatttg atcatagaag
      121 caattatcga aaaaatggag atcaagcagg ccttctaggg cgaggtcgag ctgattgccc
      181 gggataatgc gttctttgcc acgaacacct ctggattgag cataaacgag atgtgctcga
      241 agctacaaaa caaaaaacgt tttattggaa tgcatttttg gaatccccct cacattattc
      301 ccttggtcga gttgattcgc gccgatggaa ccgctgacga gaccgtcgac gacctcaagg
      361 aacttatgta tgcgatcaat aaggagccgg ttgtcgttcg gaaagacgcc cccggtttca
      421 ttgggaatcg ccttcagttc gccgtattca gagaggcgct tcacattttg gaagagggga
      481 tcgccggagt cgacgatgtc gaccgtgcca tgcggtacgg tcccggattc cgctaccctg
      541 tcatcggtcc tcttcagacg gctgatttgg gaggtttgga tacgttttat tacatctctt
      601 cgtatctctt cgcatcttta agcgatatga aggaaccttc ggttacattg aaaaacttga
      661 tggaaacaca gcagttggga gtaaaaacca agagcggatt ttacgactac tccaacggta
      721 aggatgaaga aaccataagg cgaagggata agatgttttt caagatgctg aaacatattc
      781 attcaaacgg gaagaatagc tgaaaaatac ctttggaatg tgaaaagagt gttgcagcat
      841 atgaagggta aagaaaataa gaaggatgaa agcggatgga gcgaaaaaca gcgtgattcg
      901 ctcgatggga ggcttattga tcactatata tacgttctgt cccgattgag tggaatgcag
      961 gttgcggatg tcggcgtctg tatctgtgat cttgacggcg tggttttcta caagccggcg
     1021 cgctccttgg atttgaaggt caaacatggc gacgagattc gccccggaag tctcttgtat
     1081 cgagctctgc atgagcggcg gagagtaacg gcgaagatgg actcatctct ttacggggtg
     1141 ccgtacgtag gggtgggcac ccctatcatg gacgaaggcg gtcaagtttt gggagctgtt
     1201 gcaataacgg aatcgaccct taactatgaa aacctcaaag aatcttctat gaagatagcg
     1261 gaaagcattg agatgctggc tggggcttcc gaggagatcg ccgcgcaatc tcaggaaatg
     1321 agcggtacgg ccgcgataat gaaggaaacg cttgaaaggt ccagagaaag cgtgaaggca
     1381 agcgatcagg taatagggct tatcaagatg atagcgacgc gaacgaatct tttagggttg
     1441 aacgcatcca ttgaggcggc gagggttggc gaacagggac gaggttttaa cgttgtcgcg
     1501 gaagagatca gaaaacttgc cggcgttacg ggcgattcga tacaaaatat agcgcacacg
     1561 ttggcatcca ttcagtccga caatgcggaa aactgccggc agatgctcgt catgtcttcg
     1621 ataattactc aagtgtccga tgcgacgacg cagatcgccg agaccgcgca agcgttgaac
     1681 gagatggcga gcagtctgaa cgaaatggcg gagaaaatta tggaatcgat aaactaagga
     1741 cggcgcgttt cattcgagtc cttgccactc tttccccttt ctcacggaat attcttcgac
     1801 gaatgagagg aagttccgcg cggcgaacga aaggtaattg tcctttttac tgatgaccgc
     1861 tatttcccag ataattttcg gttccagcgg tatcgagacg agcccgtcga aaatgaattg
     1921 tttgacaaca gggtatggaa gtatccctat gcaaaggccg aggtgcacca tctcgaagat
     1981 gaaattgggc atgagcgttt tatacacgat attgggctcg aagccttcgt tctcgcagtg
     2041 tcggataaac gcgtcgtgca gggttgccgt cgtactgaag gttatgatgc gctcttcgac
     2101 aagttccgac gtgtggatcg gagtgtttct tgtggcgaaa cggtgctctt tatttacaag
     2161 gacatggcag atgctcgtgg tgacaatacg gcttgtcaac ttagaggcgt tgcgaacagg
     2221 gagcatgacg aac
//
LOCUS       DAOPKU010000505         2229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_56720_length_2229_cov_5.097056, whole genome shotgun sequence.
ACCESSION   DAOPKU010000505 DAOPKU010000000
VERSION     DAOPKU010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56720_length_2229_cov_5.097056"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK849_12845"
     CDS             complement(<1..210)
                     /locus_tag="PK849_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67060.1"
                     /translation="MTAFELDWSRSAGRRRLLLIFLVLAPTFIACGTMASILPSRGGS
                     PLEIVLLAVFAVLFAWISVGFWTALM"
     gene            complement(207..563)
                     /locus_tag="PK849_12850"
     CDS             complement(207..563)
                     /locus_tag="PK849_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67061.1"
                     /translation="MTSIRDTDRWRISRDRVLLYVRALNLPPYKGLDLAVESLKRTGP
                     SSAAESMESLRALLKEHGLDRGVLDSEGKHLASVPPLNRSVMVPEELDRVPWRTSFVQ
                     FLRRWKNDLFGKGCAR"
     gene            complement(709..>2229)
                     /locus_tag="PK849_12855"
     CDS             complement(709..>2229)
                     /locus_tag="PK849_12855"
                     /inference="COORDINATES: protein motif:HMM:NF016252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HPE67062.1"
                     /translation="GSCVLYHKMFHRSAYHRAVAVLMAAVSLFLHAPAHAEGAVSSPG
                     EIPPPKVIPAEAFSFDNVVKKAEELAARPFENPDGAVPDFLLNISYDQWRKIRFRPNM
                     SLWRNEKLPFEAQFFHPGLFYNRLVGINIVHDGGALRLPFSTNFFEYGDNAFASKTGS
                     VPLDFTGFRLHFPLNRDDYKDEVAVFLGASYFRAVARGTQYGLSARGLAVDTAMQSGE
                     EFPYFREFWIEKPAQDAEQIAVCALLDSPSLAGAYRFILTPGEETVMDVECMLFLRRD
                     VQKIGIAPLTSMYLYGEPENGRDGDFRPEVHDSDGLLYHSEEGEWTWRPLANPKRLAV
                     LSAPLQNPRGFGLMQRDWLFDHYQDLEARYEKRPSLWIEPLSEWGEGNVELVEIPTEQ
                     EIHDNIVAYWVPAKMTETAEGRPKDDTPEYPRTMTFAYRMRWLQPHRPVHELGRVMAT
                     RIAAGNSPEMMKFVLDFEGDELSELPAGAGLTSIVTVGDGARLIEKQLQKNEATGG"
BASE COUNT          423 a          692 c          713 g          401 t
ORIGIN      
        1 catcagcgca gtccagaagc caaccgagat ccatgcgaag agcaccgcga agacggcgag
       61 cagcacgatc tccagagggc ttccaccgcg cgaaggcagg atggacgcca tcgtcccgca
      121 cgcaataaag gtcggcgcaa ggacgaggaa gatcagcagc agcctcctcc ggcccgcgct
      181 gcggctccag tcgagttcga acgcggtcat cgggcgcacc cctttccaaa aaggtcgttc
      241 ttccagcgtc ggaggaattg gacgaacgac gtcctccacg gaacgcggtc aagctcctcg
      301 ggaaccatca cgctccggtt gagcggcggg acggaggcga gatgcttccc ttcggagtca
      361 agcacgcccc ggtcgagccc gtgctctttc aaaagcgcgc ggagcgactc catcgactcc
      421 gccgcggagg acgggccggt gcgtttcagg ctttcgacgg cgaggtcgag ccccttgtag
      481 ggcggcaggt tgagcgcgcg cacgtagagc agcaccctgt cgcgggagat gcgccagcgg
      541 tcggtatcac ggatggaggt cataggtcca ggtctccgtc agcggttccg ggatattctc
      601 cccccgcttc agcagcgcgg agaggcggat gcgggggcgg ccggcgggcg aaggaaagag
      661 cgtcttgaga ccgctctcgg cttcgggggc aacctggaag accagccgct accctccggt
      721 agcctcgttc ttctgaagct gtttctcgat aaggcgcgcg ccgtccccga ccgtcacgat
      781 gcttgtcagg cccgctcccg ccgggagttc cgaaagctcg tccccttcga agtcgaggac
      841 gaacttcatc atctccgggg agttccccgc cgctatgcgc gtcgccatga cccggccgag
      901 ttcgtggacc gggcggtgcg gctgaagcca gcgcatcctg taggcgaatg tcatcgtccg
      961 gggatactcc ggcgtgtcgt cctttggccg gccctcggcg gtttctgtca tcttcgcagg
     1021 gacccagtac gcgacgatat tatcgtggat ctcctgttcg gtcgggatct ccacaagttc
     1081 cacattcccc tcgccccact cgctgagcgg ctctatccag agcgacgggc gtttctcata
     1141 ccgcgcctca aggtcctggt agtggtcgaa gagccagtcc cgctgcatca gaccgaaacc
     1201 gcgcgggttt tgaagcggcg cggagagcac ggcgaggcgc ttcggattcg ccagcggccg
     1261 ccacgtccac tccccctctt cgctgtggta cagcagtccg tccgaatcgt gcacctcggg
     1321 acggaagtcg ccgtcgcggc cgttctcagg ttcaccgtag aggtacatgc tcgtgagcgg
     1381 cgcaataccg atcttctgga cgtctcttcg cagaaagagc atgcactcca cgtccatgac
     1441 ggtctcctcg ccgggagtca gaataaaacg gtaggccccg gcaagactcg gactgtcgag
     1501 aagcgcgcac acggcgatct gttcggcgtc ctgcgcaggt ttttctatcc agaactcccg
     1561 gaagtacgga aattcctcgc cggactgcat tgcggtgtcg acggcgagtc cgcgcgcgga
     1621 gagaccgtac tgcgtgccgc gcgccaccgc tctgaagtag ctcgcgccga ggaacacggc
     1681 gacctcgtct ttataatcgt ccctgtttaa ggggaagtgc agccggaatc cggtgaagtc
     1741 cagcggaacc gaccccgttt tcgacgcgaa ggcgttgtct ccgtactcga agaagttcgt
     1801 ggagaaggga agtcgcagcg cccctccgtc atggactatg ttgattccga cgagcctgtt
     1861 gtagaagagg ccggggtgga agaactgcgc ctcgaagggc agcttctcgt tccgccacag
     1921 ggacatgttc gggcggaagc ggatctttcg ccactggtcg tagctgatgt tgagcaaaaa
     1981 gtcgggaacc gccccgtcgg gattttcaaa gggacgggcg gcgagttctt cggccttctt
     2041 caccacgttg tcaaaagaaa aggcttcggc cgggatgact ttcggcgggg gaatctcgcc
     2101 gggggacgaa acagccccct cggcgtgcgc gggagcgtgg aggaaaagag acaccgccgc
     2161 catgagaacg gcgacggcac gatggtaggc gcttctgtga aacattttgt gatacaagac
     2221 acaagaccc
//
LOCUS       DAOPKU010000506         2229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_56731_length_2229_cov_3.545538, whole genome shotgun sequence.
ACCESSION   DAOPKU010000506 DAOPKU010000000
VERSION     DAOPKU010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56731_length_2229_cov_3.545538"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PK849_12860"
     CDS             <1..679
                     /locus_tag="PK849_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPE67063.1"
                     /translation="RDGGFAEYVALPASNLHKVPASWSFEKAALVEPFTIAANVLSRT
                     ECAGSDKLLIMGAGPIGQVILQAARRLGTACAVSDIVGVRLEKARRLGAELTVNPKET
                     SLEEAIGAWTGGKGVPLIIDAVGSPDLFPSLLRMASPAGRIGLLGFSKEPSPFVQLEA
                     VRKELSIFGSRLNRNKFPEVISWFEEGLVEPELLVSHRFPFVQAKEAMEFIQEHPSDV
                     CKVILEF"
     gene            842..1813
                     /locus_tag="PK849_12865"
     CDS             842..1813
                     /locus_tag="PK849_12865"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE67064.1"
                     /translation="MNVFANMKTKGKILVLVFVMAILLGAVGYTGYHFNNRSAESLSE
                     VYEENLLPITWLFDALVQGQGIQSDVAQILLAHDAESREELEADIVRRVAIVDEHYAR
                     YGKLPLDAFEKEHFELALKALADYRVAWRKSKDLAGADRGYEAYQTFTTEVNPLFTAY
                     QAEIRSLAEYAQKRADETNRQNAAETVAAFRIIVGIAVGAVVLGVLLGLFIASRIAGA
                     LNRLRAGIEQFATGDLTVRFDAKGRDEAAMMAGALSDMASKLRSAMQSIAGASEKFRS
                     TAWRMPSAMPSGPGCRIPRRTSRLCTRRFRCTSCLAPPPLVRFHPSL"
     gene            2043..>2229
                     /locus_tag="PK849_12870"
     CDS             2043..>2229
                     /locus_tag="PK849_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67065.1"
                     /translation="MIIGTGAGGGAAGSKLASRGRRVLFLERGLLFSDRRAFQDEQAM
                     LMELRAADDRMHDFDGVR"
BASE COUNT          444 a          622 c          687 g          476 t
ORIGIN      
        1 cagggacggc ggttttgccg aatacgttgc gcttcctgcc tcgaaccttc acaaagttcc
       61 tgcttcatgg agtttcgaga aggccgcgct cgtcgagccg ttcaccatcg cggcgaacgt
      121 cctctccagg acggagtgcg cgggaagcga caaactgctg atcatggggg cggggccgat
      181 cggccaggtg atccttcagg cggcgcggcg gctcgggacg gcgtgcgccg tgtccgacat
      241 cgtcggcgtc cgcctggaga aggcccgtcg tctgggcgcc gagctcaccg tcaatccgaa
      301 ggaaacgtcg cttgaagagg cgataggagc gtggaccggc gggaagggcg tgccgctcat
      361 catcgacgcg gtagggtcgc cggatctttt cccctcgctg cttcggatgg cctccccggc
      421 ggggcgcatc ggactgctcg gcttctcgaa ggagccctcg ccgtttgtgc agctcgaagc
      481 tgtgcgaaag gagctgtcga tcttcggctc ccggctcaac cggaacaagt tcccggaagt
      541 catctcctgg ttcgaggaag gtcttgtcga gccggaactg ctcgtctcgc accgcttccc
      601 gttcgtacag gcgaaggagg caatggagtt cattcaggag cacccctccg atgtctgcaa
      661 ggtgattctg gagttctaaa aataccaagc gggaatgaag atacccttag gcgctgtgcc
      721 gaagggcgtc ttttttcatg cgtttcttcg gggttgcctg cgaaagggaa aacgaggata
      781 atcaatcgcg cagcgtatga tttcaaccgg aattctattt ctttaacgag gagagttgtg
      841 tatgaacgtg tttgcaaata tgaaaaccaa gggaaagatt ttagtacttg tgttcgttat
      901 ggccattctg ctgggcgctg tggggtacac ggggtaccac ttcaataacc ggagcgcgga
      961 aagtctttcg gaggtgtatg aggagaattt gctcccgatc acctggctct tcgacgccct
     1021 ggttcaaggg cagggtattc agagcgacgt tgcgcagata ttgttggcgc acgatgcgga
     1081 gtcccgggag gaactggaag cagatatcgt gcggcgggtc gccatcgtcg acgagcacta
     1141 cgccaggtac ggcaagcttc ctctcgacgc cttcgagaaa gaacatttcg agctcgccct
     1201 gaaggcgctg gccgactaca gggtcgcctg gcgcaagtcc aaggatcttg ccggcgccga
     1261 cagggggtat gaagcatacc agacgttcac cactgaagtg aacccgctct ttaccgcata
     1321 ccaggcggag attcgctcgc ttgcggagta tgcgcagaag agggcggacg agacgaatcg
     1381 gcaaaatgcc gccgagacag tcgccgcatt ccggatcatc gtgggaattg ccgtcggcgc
     1441 agtagtgctc ggcgtgctgc tggggctctt tatcgcgtcg aggattgcgg gcgctctgaa
     1501 tcggttgagg gccggcatcg aacagtttgc aacgggagac ctgaccgtgc ggtttgacgc
     1561 gaaggggaga gacgaggccg caatgatggc aggcgcgctc tccgacatgg cttcgaagtt
     1621 gcggagcgcg atgcagtcca tcgccggagc atcggagaaa tttcgatcaa ctgcgtggcg
     1681 aatgccgtct gccatgcctt ccggtcccgg ttgtcgaatc ccacgcagaa ccagccgcct
     1741 ttgcacccgt cgattccgat gtacttcatg tctcgccccg cctcccttgg tccgtttcca
     1801 tccctctcta taaatttaaa cggttccggc aaatcctgcc agttttttca ccggaccggc
     1861 actccttttc ccttcagcat tccgggctaa caggattcta atttgacagg atagaacccg
     1921 ctcaataccg ttcccgaatg caaaagcagt atcgtaacct gctgatataa attattttcg
     1981 atcgagctaa catgcactca cacgcggttt attcacagcc acagcacagc cactgggatg
     2041 ccgtgatcat cggcaccggc gccggtggcg gcgcggccgg ctcgaagctg gcatcccggg
     2101 gccggcgggt gcttttcctg gaacggggcc tgcttttttc cgaccgccgg gcgttccagg
     2161 atgaacaggc catgctgatg gaattgagag ccgccgatga tcggatgcat gatttcgacg
     2221 gagtccgga
//
LOCUS       DAOPKU010000507         2222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_57005_length_2222_cov_4.276419, whole genome shotgun sequence.
ACCESSION   DAOPKU010000507 DAOPKU010000000
VERSION     DAOPKU010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57005_length_2222_cov_4.276419"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PK849_12875"
     CDS             complement(<1..685)
                     /locus_tag="PK849_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPE67066.1"
                     /translation="MSIQLPVLKLGKHTPRYPLIQGGMGVAISGPSLAGHVAKCGGVG
                     TIASVGLAANSPHYNGRNYFDANQLAVKDAIADARKIAGPGGVIAVNCMVALTDYDRQ
                     TVSACQGGVDVIISGAGLPLRLPEYTRDFPDVALVPIVSSLKAAALITRRWEKTYGRV
                     PDGFVVETPKYAGGHLGVTKMDQVDDEQFTLERVIPELVEFVEKEMGSDIPVIGAGGI
                     WDREDMEKVF"
     gene            918..2216
                     /locus_tag="PK849_12880"
     CDS             918..2216
                     /locus_tag="PK849_12880"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPE67067.1"
                     /translation="MFTVKEFLKSEVKPALGCTEPGAVALAVARAKEELPGDVESVVV
                     TVSDSIYKNGVDVGIPGTKGLRGNNVAAALAVLCGRSEYGLEVLKDCAEEDAAKALEL
                     LGKGCIKVIPDMERHGVFVEAKIQSGGKTALCRIEGSHTNIVEVLLDDKIRFSTVCPE
                     STNISEQAKSISEEVSRMGFSELVSMVDSMDEEDIDYVLKGVEMNMHIAEYGFSPDHD
                     SGLNLGKTIRKHSGTGFDSDDLSLKIKSYAAAAADARMSGAPLPVMSSAGSGNHGVTA
                     ILPVAIFAAHLGADKEDTAKAVAFSHLATSYVKSRTGRLTPTCGCTVAAGAGAAAGMT
                     YLKTKDIAKAAQAMLIILGNLVGMVCDGAKYTCALKVGTGALEAYHAALLVFDGHYPE
                     TQGLVDETIEKTVNNMVQVCVHGMQNLDKAIIDVISHRFN"
BASE COUNT          525 a          641 c          605 g          451 t
ORIGIN      
        1 aaaagacctt ttccatatct tcgcggtccc atatgccacc ggcgccaatg accgggatat
       61 cgcttcccat ctctttctcg acgaactcga caagttcagg gataactcgc tcaagggtaa
      121 actgctcgtc atccacctgg tccatcttcg tcactccgag atgtcctccg gcgtacttcg
      181 gcgtctcgac gacgaaaccg tcgggaacac gtccgtacgt cttctcccat ctgcgggtga
      241 tcagcgcggc ggctttcaac gagctgacga tcggaacgag cgccacatcc gggaagtcgc
      301 gagtgtactc gggcaggcgg agaggaagcc ccgctccgga gatgatgacg tcaacgccgc
      361 cctggcaggc ggaaacagtc tggcggtcgt agtccgtaag cgccaccata cagttgacgg
      421 cgattactcc gccgggaccg gcgattttcc gcgcatctgc aattgcgtct ttgactgcca
      481 gctggttcgc gtcaaaatag ttgcgaccgt tgtagtgtgg ggagtttgcc gcaagtccta
      541 cgctggcgat cgttcccacc ccgccgcatt tcgccacatg ccccgcgaga ctgggtcctg
      601 aaatcgcaac tcccatgccg ccctgtatca agggataccg gggcgtatgt tttcctaact
      661 ttaaaacggg cagttgtata ctcaaaaaga ttctctcctc tgcaaagtgt tctctggatc
      721 ttgcccttca aacaaagggc tatcatattc ttctattgta tcttatagtg aacaacacga
      781 gcaagaaaaa aatatagctt ctttcccgtg tttcgcactg tccccaccaa gaaacgattg
      841 acgtgctttt cgttgcacgg taccatttgt ctagtatata ccctgattct caaattttac
      901 tgcgtggagg ggacaaaatg ttcaccgtca aggaattttt gaagtccgaa gtaaaacccg
      961 ccctcgggtg taccgagccc ggagcggtgg cattggctgt cgcccgggcg aaagaagagc
     1021 tgccgggaga tgtggaaagc gttgtcgtga ctgtaagcga cagcatctac aagaacggcg
     1081 tggacgtagg gattccgggc actaagggtc ttcgcgggaa caatgttgcc gcggcgctcg
     1141 ccgtcctctg cggacgatcg gagtacggcc tcgaagttct gaaggactgc gcagaagagg
     1201 acgcagcaaa ggctttggag ctgctgggca aggggtgtat caaggtaatc cccgacatgg
     1261 agcggcacgg agtctttgtc gaagcaaaaa tccaatcggg cgggaaaacc gctctctgcc
     1321 gcatcgaagg aagccacacc aacatcgtgg aagttctgct cgacgacaaa atccgcttct
     1381 ccacagtatg ccccgaatcc acaaacattt cggaacaggc aaagagcatc tccgaggaag
     1441 tgtcgcggat gggattctcg gagctcgtgt cgatggtgga ctccatggac gaagaggata
     1501 tcgactatgt cctcaaaggc gtggagatga atatgcacat cgcggaatac ggtttctccc
     1561 ccgaccacga ttcgggactc aatctcggta aaaccattcg aaagcactcc ggaacaggat
     1621 tcgattccga cgacctcagc ctgaagataa aatcctacgc cgcggctgcg gcggacgccc
     1681 gcatgtcggg agctcccctc cccgttatga gcagcgcagg cagcggcaac cacggagtca
     1741 ccgccatcct tccggtggcg atcttcgccg cgcacctggg cgcggacaag gaagacaccg
     1801 cgaaagctgt cgcattcagc cacctcgcca caagctatgt aaaaagccgc accggacgcc
     1861 tgacgccgac ctgcggctgc actgtcgccg ccggcgccgg cgctgcggcg gggatgacct
     1921 acctgaagac aaaagatatc gccaaagcag ctcaggctat gctgatcatt ctgggaaacc
     1981 tcgtcggtat ggtctgcgac ggagcgaaat acacctgcgc actgaaagta ggaaccggcg
     2041 cgcttgaagc gtatcacgca gcgctgcttg tcttcgatgg acactacccc gagacccagg
     2101 ggcttgtaga cgaaaccatc gaaaaaaccg tgaacaacat ggtccaggtt tgcgtccacg
     2161 gcatgcagaa tctcgataaa gcaatcatcg acgtcatctc acaccgcttc aattaacgga
     2221 cg
//
LOCUS       DAOPKU010000508         2222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_57014_length_2222_cov_3.553299, whole genome shotgun sequence.
ACCESSION   DAOPKU010000508 DAOPKU010000000
VERSION     DAOPKU010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57014_length_2222_cov_3.553299"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /gene="acpP"
                     /locus_tag="PK849_12885"
     CDS             complement(<1..197)
                     /gene="acpP"
                     /locus_tag="PK849_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPE67068.1"
                     /translation="MKKEEMLARLKEIIIDRLDVEEDQIVAEASFVEDLGADSLDIVE
                     LIMGIEEEFDIEIPDEDAEKLT"
     gene            complement(225..965)
                     /gene="fabG"
                     /locus_tag="PK849_12890"
     CDS             complement(225..965)
                     /gene="fabG"
                     /locus_tag="PK849_12890"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPE67069.1"
                     /translation="MEQRIALVTGAGKGIGRAVAIRLAKNGFFVAVNFRSSEKAALEV
                     CDIIAAGGGSAFPVKGDVSDPEEVKRVFSFVNEKAGNVSVLVNNAGITKDALLIRMKD
                     EDWNSVLTANLSSVYYCTREALRGMVRAKWGRVINIASVVGLIGNPGQANYCASKAGV
                     IGFTKSAAREYAAKGITVNALAPGFIGTDMTDALPQEARETLLKQVPAGRPGTPEDVA
                     NIACFLASDEASYITGQVIAVDGGMTMC"
     gene            complement(967..1914)
                     /gene="fabD"
                     /locus_tag="PK849_12895"
     CDS             complement(967..1914)
                     /gene="fabD"
                     /locus_tag="PK849_12895"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPE67070.1"
                     /translation="MSYSLIFPGQGSQEVGMGRDIRDAFKSAAEVFQRADDALSFSLS
                     KIIFDGPEEELRRTAYTQPAILVASIAAISVLEELLGKRLQPASLAGHSLGEYTALVA
                     ARTLSLEDGVRLVHLRGRLMQEAVPEGKGAMAAILGLDAQAVSDICQEVAPLHECQPA
                     NFNSPGQIVISGETEFVEKAMLLAKEKGAKKTVLLNVSAPFHSRLMRPVADQLLTAFK
                     QCAWGNPSAPIVSNVRALPVANIQDIQDALFHQTYMPVLWEKSVHWMADSGVDTFFEI
                     GPGTVLGALVKKCRKGLTAYPCGTLATLEKTAALLKEMM"
     gene            complement(1924..>2222)
                     /locus_tag="PK849_12900"
     CDS             complement(1924..>2222)
                     /locus_tag="PK849_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPE67071.1"
                     /translation="NVITGRITGHPVRCLKNKLTSRFEELEKENAPVEEYEKLGAGRL
                     RAAVVEGDVEWGSLMAGQSAALVNDILPAAEIIRNLFRDAEIISQGLSAALSLE"
BASE COUNT          513 a          632 c          524 g          553 t
ORIGIN      
        1 gtgagtttct cggcgtcttc atcaggaatc tcgatatcga attcctcttc gattcccata
       61 atgagttcga caatatcaag ggagtccgcc ccaaggtctt cgacaaagga agcttcagca
      121 actatctggt cctcttccac gtcaagcctg tcgataataa tctccttcaa ccgagcgagc
      181 atttcttctt ttttcattct cgttcacccc ccttcactct ctttttagca catggtcatg
      241 ccgccgtcga cggcaataac ctgtcccgtt atatacgacg cttcatcgga agcaaggaaa
      301 cacgcgatat ttgcaacgtc ttcgggtgtt cccggccttc cggcgggcac ttgtttcaaa
      361 agagtttccc tcgcctcctg cggcaacgca tcagtcatat ccgtgccaat gaaaccgggt
      421 gcgagagcgt ttacggtgat cccttttgcg gcatactctc tggcggccga ttttgtaaaa
      481 cctatgactc cagcttttga ggcgcaatag ttcgcctgcc cggggttgcc tatcaggccg
      541 acaaccgagg cgatatttat gacacgtccc cacttggccc gtaccattcc cctgagagct
      601 tctctggtgc aataatacac cgaagagaga ttcgctgtca gcaccgaatt ccaatcttcg
      661 tctttcattc tgatgagcag ggcgtccttt gtaattccgg cattgttcac gagtacgctt
      721 acgttcccgg ctttttcatt gacaaaagag aatacccgtt ttacctcttc gggatcggac
      781 acatctcctt tgacggggaa ggcgcttcct cctccggcgg caatgatatc acaaacttcc
      841 aaggctgcct tctcgctgct gcgaaaattc acggcgacaa aaaaaccgtt cttcgccagg
      901 cggattgcca cagcccgccc gatccctttg cctgcgcctg ttacaagagc gatgcgctgc
      961 tccatatcac atcatctcct ttaaaagagc cgctgttttt tcaagggtag caagagttcc
     1021 gcacggatat gccgtgagac ccttgcggca cttcttcacc aatgctccca aaactgtacc
     1081 ggggcctatt tcaaagaaag tgtctacccc ggaatctgcc atccagtgaa cagatttctc
     1141 ccagaggacc ggcatatacg tttgatggaa gagggcgtcc tgtatgtctt gtatgttggc
     1201 tacaggcagc gcgcgcacgt tggagacaat aggtgcggag ggattccccc acgcgcactg
     1261 cttaaaagct gtaagaagct gatctgccac agggcgcatc aaacggctgt ggaagggagc
     1321 gctgacattc aagagcactg tttttttggc gcctttttct tttgccaaaa gcatcgcttt
     1381 ctcaacaaat tctgtttctc cggagatgac aatctgaccg ggagaattaa agtttgcggg
     1441 ctgacactcg tgcaaaggag cgacctcctg gcaaatgtcg gagacagcct gcgcatcaag
     1501 tccaagtatt gccgccatcg cgcccttgcc ctcaggaacc gcttcctgca tgagcctccc
     1561 acgaagatga accaggcgga cgccgtcttc aagagagagc gttcttgcag ctaccagagc
     1621 cgtatactcc ccgagactgt gtcctgcgag agaagctggt tgaagacgtt tccccaaaag
     1681 ttcctccaaa acggagattg cggctatgct tgcgacgaga atggcaggtt gcgtatatgc
     1741 cgtcctccgc agttcctctt cagggccatc aaaaattatc tttgaaagag aaaaggagag
     1801 tgcatcatcg gcacgctgaa agacttccgc agcactttta aatgcatcac gaatgtctct
     1861 gcccatccct acttcttgag atccctgacc ggggaagatc agcgaatatg acatcttttc
     1921 acctcattcc aaagaaagag ctgcagaaag cccctgactg attatttctg catcacgaaa
     1981 aagattccgg ataatctctg cggcaggaag gatatcgttg accagggcgg cgctctgtcc
     2041 agccatgaga gacccccatt cgacgtcgcc ttcgacaacg gcggcgcgaa gacgtcctgc
     2101 tcccagcttt tcatactcct cgaccggcgc gttctctttt tcaagctctt caaaacggct
     2161 ggtaagcttg ttttttagac agcgcacagg gtgcccagta atacggccgg taatgacgtt
     2221 gc
//
LOCUS       DAOPKU010000509         2221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_57043_length_2221_cov_5.434903, whole genome shotgun sequence.
ACCESSION   DAOPKU010000509 DAOPKU010000000
VERSION     DAOPKU010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57043_length_2221_cov_5.434903"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PK849_12905"
     CDS             <1..570
                     /locus_tag="PK849_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HPE67072.1"
                     /translation="LIRDVAESTLREVIASRALDDVLTTEKEASQTEAKMLIQGKLNA
                     LKTGVFIVAVQFQDVTPPKAVQAAFSEINTARSEQERLILEADKYSNEIISKAQGESE
                     RILNEAEAYKFRRISLAEGEASRIRSLNAAYKENPDLVLNNLWLESMVKVWPKLKVFI
                     VDGGESTVQLLPLEEFFKSAGAGAPETER"
     gene            584..1438
                     /locus_tag="PK849_12910"
     CDS             584..1438
                     /locus_tag="PK849_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPE67073.1"
                     /translation="MKKYLAAIGVLFVFLLFSGSFYLLRADEQGVVLRLGKVVASRTE
                     AGLYFKLPFLDQLTRYSKKLIEYDAELVAVVTSDKKNLVFDTFAIFTISDPELFYRRV
                     RTVASVQQRLDDAVYSAVRVISGRLTLDELVKDKRQQAVEQSLAIAREQANEYGVHIH
                     SLAYKRVFLPQDNEQAVYRSMQAERNRVAGQLRAEGQAEAITRRSLADRKDVEMIAEA
                     RRKAEEIKGQGDSIAQETLSKATSDAKELYLFIKTMDFYQEVLPGTPVLLRPGDGILR
                     YLTGVQGK"
     gene            1510..2151
                     /locus_tag="PK849_12915"
     CDS             1510..2151
                     /locus_tag="PK849_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPE67074.1"
                     /translation="MSEFTPWHNETVGGAVVEALKKNGFEAEYCATGADAAQRLLELI
                     PEAASVGFGGSWTLKAIGISEKLAERGNTILDHGAPGLTNEEKADIRRKQQICDVFLS
                     GTNALTMDGRLVNMDGSGNRVAAMIFGPGKVIVVAGTNKIVKDLAAAEERIQMKAAPI
                     NNKRLGTPNPCSRTGVCMDCQGPTRICNVLTIISKRPLGTNFHVLIVGEELGF"
BASE COUNT          499 a          662 c          678 g          382 t
ORIGIN      
        1 ctgatccgcg acgttgccga atccactctg cgcgaggtta tcgcctcacg tgcgctcgac
       61 gacgtgctca ccacggagaa ggaggcctcg cagacggagg ccaagatgct catccagggg
      121 aagctgaatg cgctgaagac cggggtcttc atcgtcgcgg tgcagttcca ggacgtaacc
      181 cccccgaagg cggtccaggc cgcgttcagc gaaatcaaca cggcgcgctc cgagcaggag
      241 cggctgatcc tggaggcgga caagtactcc aacgagatta tctcgaaggc gcagggcgaa
      301 tcggaacgta tcctgaacga ggctgaagca tacaaattcc gccggatatc gctcgcggag
      361 ggcgaagcct cgcgcatccg ttcgctgaac gcggcgtaca aggagaatcc cgacctggtg
      421 ctcaacaatc tctggcttga gagcatggtc aaggtctggc cgaagctgaa ggttttcatc
      481 gttgatggcg gagagagtac ggtgcagctt cttccgcttg aagagttttt caaatccgcg
      541 ggagccggcg ctcccgaaac cgagagatag gaggccggag acgatgaaaa aatatcttgc
      601 agccataggc gtcctgtttg tttttctgct gttcagcggc tctttctacc ttctgcgggc
      661 cgacgagcag ggcgtggtgc ttcgtctcgg gaaggtagtt gcctcacgaa cggaggcggg
      721 actctacttc aagctcccct tcctcgacca gcttacgcga tactcaaaaa agctcatcga
      781 gtacgacgcg gagctcgtcg ccgtcgtcac cagcgacaag aagaacctgg tgttcgacac
      841 gtttgccatc ttcacgatct ccgatccgga gctcttctac cgtcgtgtcc ggacggtcgc
      901 ttcagtccag cagcgcctcg acgacgcggt gtactccgcg gtgcgcgtca tctcgggccg
      961 tcttactctt gacgagctgg tcaaggacaa gcggcagcag gcggtagagc agtcgctcgc
     1021 catagcgcgc gagcaggcga acgagtacgg cgtgcatatc cactcgctgg cgtacaaacg
     1081 ggtcttcctc ccgcaggaca acgagcaggc cgtctaccgc tcgatgcagg cggagcgcaa
     1141 tcgcgtcgcg ggacagctgc gggcggaggg acaggcggag gccatcaccc ggagatcgct
     1201 cgcggacagg aaagatgtgg agatgatcgc cgaagcgcgc agaaaggccg aggagatcaa
     1261 ggggcagggg gacagtatag cgcaggaaac gctttcaaaa gcgaccagcg acgcgaagga
     1321 gctctacctt ttcatcaaga cgatggattt ctaccaggaa gtccttcccg gaacgccggt
     1381 cctgctccgc ccgggcgatg ggattctccg gtaccttacc ggcgtacagg gaaaataagc
     1441 ggacattgag aaggaacgga gagttcgctc cccgctcctt tcagaaaaac aaaacaagga
     1501 ggtctatcaa tgagtgaatt cacaccctgg cacaatgaaa cagtcggcgg agctgtcgtc
     1561 gaagccctga agaagaacgg cttcgaggcc gagtactgcg cgaccggagc ggacgccgca
     1621 cagcgccttc ttgaactgat ccccgaggcc gcatccgtcg gattcggggg ttcgtggacc
     1681 ctcaaggcga tcgggatatc cgagaagctc gccgagcgcg gcaacacgat cctcgaccac
     1741 ggcgctcccg gcctgaccaa cgaggagaag gcggatatcc gcaggaagca gcagatctgc
     1801 gacgtcttcc tctccggcac gaacgcgctc accatggacg gcagactcgt caacatggac
     1861 ggctccggaa acagggtcgc cgcgatgatc ttcggccccg gcaaggtcat cgtcgtcgcg
     1921 ggaacgaaca agatcgtcaa ggatctggcc gcagccgagg agcgcatcca gatgaaagcc
     1981 gcccctatca acaacaagcg gctgggcact cccaacccct gttcgcggac cggcgtctgc
     2041 atggactgcc agggaccgac caggatctgc aacgtcctga ccatcatcag caaacgcccg
     2101 ctcgggacca acttccacgt gctgatcgtc ggcgaagagc tgggattcta gaacgcctcg
     2161 caatacagaa cagggggaga cgatgcggaa atgctcgtct ccccctgttc ttttcaacct
     2221 t
//
LOCUS       DAOPKU010000510         2214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_57270_length_2214_cov_4.529412, whole genome shotgun sequence.
ACCESSION   DAOPKU010000510 DAOPKU010000000
VERSION     DAOPKU010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57270_length_2214_cov_4.529412"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1115)
                     /locus_tag="PK849_12920"
     CDS             complement(237..1115)
                     /locus_tag="PK849_12920"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPE67075.1"
                     /translation="MKLVKGKSTVGFIGLGVMGHSMAGHVLRGGFDVLVYNRSKQKTL
                     DLLTQGAVWKDDIFSLAEKSDVVITMVGYPKDVEEVYLGERGIVAAARPGTVLIDMTT
                     SSPKLAERIASAAAEKGLFSLDAPVSGGDKGAREATLSIMIGGEETVFEDMLPLFQLM
                     GKNIVLQGGPGSGQHTKMANQIAIASNMMGVCEALAYAKKSGLDTGRVLSSISGGAAG
                     SWSLSNLAPRILAGNFAPGFFVKHFIKDMRIALEEADRMGMDAPGLKQAFALYEELAA
                     KGCENDGTQALFKLYE"
     gene            1276..>2214
                     /locus_tag="PK849_12925"
     CDS             1276..>2214
                     /locus_tag="PK849_12925"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HPE67076.1"
                     /translation="MKTIERIEQLLGNEAKVLLEHKCATIDKSMLTLPGPDFVDRVTA
                     ISDRSIPVMRSMQTLFNSGRLAGTGYLSILPVDQGIEHSAGASFAPNPIYFDPENIVK
                     LAMEAGCNAVASTLGVLSSVARKYAHKIPFVLKINHNELLTYPNKHDQILFASVEQAR
                     DMGAVAVGATIYFGSDEATRQIQEISNAFEIAHELGMATILWCYLRNSAFKTAEKDYH
                     VSADLTGQGNHLGVTIGADIIKQKLPENNGGYTALKFGKTHKNVYDKLSSDHPIDLTR
                     YQVANCYMGRAGLINSGGASGGESDMAEAVKTAVINK"
BASE COUNT          476 a          694 c          590 g          454 t
ORIGIN      
        1 ctgtcgtgaa ggagaatatc aataattaga cgccgctggg gcgtgagctt cagccccttt
       61 ttccgaagct gttcggcgat ctgttcgact gagcgcatga gtgtcacttc ctttctcgtg
      121 atgcaacttg taaccattat aaacaaaaac aatttttgcg tcaatccaca aataaacaag
      181 gcccttcgaa ggaccttgcc gtcggagaat atctcctgcc ttacaaccgg gagatcttat
      241 tcatagagtt tgaagagcgc ctgggttccg tcgttttcgc accccttggc cgcaagctct
      301 tcgtagaggg cgaacgcctg tttcaacccc ggcgcgtcca ttcccatacg atcggcttct
      361 tccagagcga ttcgcatatc cttgatgaag tgcttgacga aaaaaccggg ggcgaaattc
      421 cctgcaagaa ttctgggagc gaggttcgac agcgaccagc ttccggcggc tccccccgag
      481 atgctggaga ggacgcgccc ggtgtcgagt cctgattttt tcgcgtacgc caaagcttcg
      541 cagacgccca tcatgttgga agcaatagcg atctgattgg ccatcttcgt gtgctgaccg
      601 cttccgggac cgccctgcag gacgatattc ttccccatca gctggaagag cgggagcata
      661 tcctcgaaaa cagtctcctc gccgccgatc attatggaga gcgtcgcttc gcgggctccc
      721 ttatctcccc cggatacggg cgcgtcgagc gagaagagcc ccttctccgc cgccgccgag
      781 gcaatgcgct ccgcaagctt cgggctcgac gtcgtcatgt cgataaggac tgtgccggga
      841 cgggcggccg caacgattcc cctctcgccg aggtagacct cctccacatc cttcgggtac
      901 cccaccatgg tgatgacaac atcgctcttc tcggcgagag aaaaaatgtc gtccttccag
      961 acggccccct gtgtcagcag gtcgagtgtc ttttgcttgg aacggttgta gacaagtacg
     1021 tcgaaccctc ctcgcagtac gtgtccggcc atgctgtgcc ccataactcc aaggccgatg
     1081 aagccgaccg tcgacttccc cttaaccagt ttcattcccc cacctccggc gaatattcgg
     1141 cgcttttttt cgtttttttg ttttcttcat tcacagagcg ccctttgtcg ggtacaatct
     1201 cctcaaaagg gtttctcttc gagggatcgt agtcattgta ccctcgttat ttcagattga
     1261 aaaggaggca gattcatgaa gactatcgaa cggattgaac aactgcttgg aaacgaagca
     1321 aaagtgctcc tggagcacaa gtgcgcaacc atcgacaagt cgatgctcac ccttcccggc
     1381 cccgactttg tcgaccgggt caccgcgatc tccgaccgct ccatcccggt gatgcgctca
     1441 atgcagacgc tcttcaactc ggggcgtctg gcgggaacgg gctacctctc catcctcccc
     1501 gtggaccagg ggatcgaaca ctcggccgga gcaagctttg cgccgaaccc gatctacttt
     1561 gatccggaga acatcgtcaa gctggcgatg gaagccgggt gcaacgccgt ggcgagcacg
     1621 ctcggcgtcc tctcctccgt ggcgcggaag tacgcccaca aaatcccctt cgtgctgaag
     1681 atcaaccaca acgagctgct cacgtacccg aacaagcacg accagattct cttcgcgtcg
     1741 gtggagcagg cccgcgacat gggcgccgta gctgtcggcg cgacgatcta cttcggcagc
     1801 gacgaggcca cccggcagat ccaggagatc agcaacgcct tcgagatcgc gcatgagctc
     1861 ggcatggcga ccatcctgtg gtgctatctg cgcaacagcg ccttcaagac cgccgagaag
     1921 gactaccatg tatcggccga cctgacggga caggggaacc acctcggcgt gaccatcggc
     1981 gccgacatca tcaagcagaa gctcccggag aacaacggcg gctacaccgc gctgaagttc
     2041 ggcaaaacgc acaagaacgt ctacgacaag ctctccagcg accaccccat cgacctcacc
     2101 cgctaccagg tcgccaactg ctacatgggc cgggcgggac tgatcaactc aggcggcgct
     2161 tcgggcggcg agagcgacat ggccgaggcc gtgaagaccg ccgtgatcaa caag
//
LOCUS       DAOPKU010000511         2210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_57428_length_2210_cov_4.286775, whole genome shotgun sequence.
ACCESSION   DAOPKU010000511 DAOPKU010000000
VERSION     DAOPKU010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57428_length_2210_cov_4.286775"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PK849_12930"
     CDS             complement(<1..981)
                     /locus_tag="PK849_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HPE67077.1"
                     /translation="MKILTAPSNGGFVREGALALFVFSKESVSGGLLDEHFADMAARL
                     MEEPSFRGKKGKISRLPLLNGGFSAAYLVGLGDRAKNGKESALHENIRSRSAELLRRC
                     RADGVSKITVVLPDAPSFGTSCALAEGAELGAYAFSKYKSIPEEERLPEPAELLLLDG
                     VDEGVSRGKMFACSQNWAKDIANEPGNKITPVSMADMAVQWGKDFGFEVSVWDEQRLL
                     QEGMEGIYSVGMGSAHPPRLIHMVYRPSDSVRKVALVGKGVTFDSGGLNVKTGDSMRT
                     MKGDKTGASNVFAILRGAAKMNIPCELHGIVPVAENMPGGKSFRPDDILRI"
     gene            complement(1064..1975)
                     /locus_tag="PK849_12935"
     CDS             complement(1064..1975)
                     /locus_tag="PK849_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE67078.1"
                     /translation="MTPSSVARLKALVAVIVWGASFPSAKVAVGEVSFTTLVWLRFGS
                     GLLVLFLFLAIRGKIRLLPLREAVTFAALGFLGVFFHNSIQAYALQTVSAGMSGLIIA
                     ANPIAIALLGSLLLSEPLGKAKIAGILLAACGVLVIISRGNISVFLQREYSFGELVMV
                     LSVFSWGFFSVLSRKALQRTSPELGMTYALTFGWLFSIIPFLANGGVAELPSVSGKAW
                     ANILFLGILCSALAYLFWYDALQVLPASEVGVFLYVNPVVAVIISALFLGESVSASML
                     FGGAMVFSGVWCVNSLSRMQEKRRKRA"
     gene            complement(1972..>2210)
                     /locus_tag="PK849_12940"
     CDS             complement(1972..>2210)
                     /locus_tag="PK849_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67079.1"
                     /translation="PSVKRILEINPHHKVIRSLEQRLSKGEGKESLGEYVFLLYDQCL
                     LAEGGRVEDPALFARRVTAILAESLKDEDGTEER"
BASE COUNT          569 a          662 c          535 g          444 t
ORIGIN      
        1 gatacgcagt atatcgtccg ggcgaaacga tttgccgccg ggcatgtttt cagcgaccgg
       61 gacaatcccg tgcagttcgc aggggatgtt catcttcgcg gcgcctcgaa gaatagcaaa
      121 cacatttgac gctcccgttt tgtcgccttt catcgtccgc atagaatctc cggtctttac
      181 gttcagtcct ccgctgtcga acgtgacgcc tttcccgacg agcgcgacct ttcgaacaga
      241 atccgacggc ctgtacacca tgtgaatcaa ccgaggcgga tgcgccgaac ccattccaac
      301 ggaatagatc ccctccattc cttcttggag aagccgttgc tcatcccaga cggaaacctc
      361 gaaaccaaaa tccttccccc attgaacagc catgtccgcc attgacaccg gggtgatttt
      421 gtttcctggt tcatttgcga tatctttcgc ccagttctgt gagcaggcaa acatcttgcc
      481 ccggctcacg ccttcgtcga cgccgtcgag cagaagaagt tccgcaggct cgggaagtcg
      541 ttcctcctcg ggaatgctct tgtacttact aaaagcgtat gctcccagtt cggcgccttc
      601 cgcaagagcg cagctcgtcc cgaatgaagg cgcatccggc aggacgacag tgattttaga
      661 aactccgtcg gccctgcacc gccgcagaag ttcggcgctt ctcgatcgga tattctcatg
      721 aagagcagac tctttgccgt tctttgcacg gtccccgaga ccgacaaggt acgccgcgga
      781 aaatcccccg ttcagaaggg gcaatcgcga tattttccct ttttttcccc tgaaagaggg
      841 ctcttccatc agccgtgcgg ccatatccgc aaagtgctca tcaagcaaac caccggatac
      901 ggattctttg gagaacacga acagagccaa agctccttca cgaacaaagc ccccgtttga
      961 gggggctgta agaattttca ttgaacgcaa ctctcctttc cagcttatga atcctcgacc
     1021 tcaatacctt ctccagttct tcactcgtta ttcggtagag acgctacgcc cttttccgcc
     1081 gtttttcctg cattcgggac aggctgttca cgcaccagac gccggaaaat accattgctc
     1141 cgccgaaaag catcgatgca ctcacagact ctccaaggaa caacgcggag ataatcaccg
     1201 caaccacagg attgacgtaa aggaaaacgc ctacttcaga cgcggggagt acctggagcg
     1261 catcgtacca gaacagatag gccagcgcag agcaaagaat gccaagaaaa aggatattcg
     1321 cccacgcttt gcccgaaact gacggaagct cggccactcc gccattcgca agaaagggaa
     1381 taatggaaaa caaccacccg aacgtcagcg cgtaggtcat accgagctcg ggcgacgtcc
     1441 gctgcagggc ctttcgcgaa agcaccgaga aaaatcccca cgaaaagacg ctcaacacca
     1501 tgacgagctc accgaaggaa tactcccttt gaagaaaaac agaaatatta ccgcgggaga
     1561 taatgacgag cactccgcac gcagccagca gaatacctgc gattttggct ttgccaagag
     1621 gttcggagag aaggagtgaa ccaagaagcg ctattgctat cggatttgct gcgataatca
     1681 accctgacat cccggcggac acagtctgca acgcataggc ctgtatcgag ttatggaaaa
     1741 agaccccaag aaaaccgagc gcggcgaacg ttacagcctc acgcaacgga agaaggcgga
     1801 tttttccccg tatcgccagg aaaagaaaca gcacgagcaa tcccgagcca aagcgaagcc
     1861 atacaagcgt tgtaaaggag acctccccca cagcgacctt tgcggacggg aaggaggccc
     1921 cccaaacgat gacagcgaca agcgctttca agcgagctac agaggaagga gtcacctttc
     1981 ctccgttccg tcttcgtcct tcaggctttc cgccaggatt gcagtcaccc tgcgagcgaa
     2041 aagcgccgga tcctcgactc gaccgccctc tgcgaggaga cactggtcgt agagaagaaa
     2101 tacgtactcc cccaacgatt ccttcccctc tcctttagaa agacgctgct ccagggaacg
     2161 aatcactttg tggtgaggat ttatctcaag gatccttttc accgaaggaa
//
LOCUS       DAOPKU010000512         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_57800_length_2201_cov_4.856477, whole genome shotgun sequence.
ACCESSION   DAOPKU010000512 DAOPKU010000000
VERSION     DAOPKU010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57800_length_2201_cov_4.856477"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..1150
                     /locus_tag="PK849_12945"
     CDS             389..1150
                     /locus_tag="PK849_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPE67080.1"
                     /translation="MRLVLYNIRYGTGTGWSYHLPFPFSGCLRRTEGRFRRISEYVSK
                     LNPDLVGLVEADSGSFRQNGSCQAKALASRIGGESVFACKYDSGSIVSKTPLLKSQGN
                     AVITKLPMIGTKEHVLSKGVKKTLLEVEFDDFTLFLTHLPLGYAARSVQLGEIAERCI
                     DAKKPVILAGDGNTYRGERELRSFFRKTGFQNANPTNTPTFPSRIPALALDFVLYGPG
                     IEMERFDVPRVRYSDHLPLVCDFTVARPKKERHSQ"
     gene            1147..>2201
                     /locus_tag="PK849_12950"
     CDS             1147..>2201
                     /locus_tag="PK849_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPE67081.1"
                     /translation="MTVTMLLYSAYELSSIAIRLIMLCYIPLKHTPSTATAWLLAIYF
                     WPWLGFLLYSLFGSTSLPQERIDRHETMLRSLDEDKRMFRRIFDEEAHWLPDELVRFG
                     ALGRKLGDMCITRGNDFLLIDREEAFIRSLAADVENAQRSVHLLFYIFARDAVTEPLF
                     RALEGAAARGVACRVLVDALGAKPFLKRDAERLRAAGIRVEKALPPRLLRRTPTTARY
                     DLRNHRKIAVIDGRVGYTGSHNVTEPSYGGKAKGLLWRDLTLRLVGPVVLQLQGVFME
                     DWFVETREMLLSDEAFPAPEYAGEAVVQTVPSGPSYPWQNYQRLVVSALHGAKKRVVI
                     TTPYLIPDDELLHALETA"
BASE COUNT          402 a          673 c          652 g          474 t
ORIGIN      
        1 gctcatcgcg cctgttccgg agtcggtgag gaggtcgata tagacgtccg cgccgcggag
       61 accgaacatg ttgaaacggg ctgcttcaag cgctttctcg cgctcgtcct tgccgggaac
      121 gcggaccggt tctaccatct tgatgcgaaa aggctcgggg ggtaccttca cgaccatgaa
      181 ttttttcctc cttttcaatt gagaaaagat caggtttctt ccgttattct accctatatt
      241 ctcctgtttt gctatttttt tcgcatcatc ggtgaaaaac tttttgcagt gcctgccggg
      301 aggtttggag aaacagcagg aatcaccgta gaggaatggc ggaggtgtta tataatgaac
      361 gatattttct gtgtttaggg aggctcatat gcgtttagtg ttgtacaaca tcaggtatgg
      421 aaccggaacc ggatggagct atcaccttcc gtttcctttc tcgggatgtc ttcggaggac
      481 ggaggggcgc ttccgccgta tctcggagta tgtctccaaa ctgaaccccg atcttgtcgg
      541 gctggtggag gcggattccg gatcgttccg tcagaacggc tcctgtcagg cgaaggcctt
      601 ggcctcccgg atcgggggcg agtcggtttt cgcatgcaag tacgactccg gctccatcgt
      661 ttcgaagacg ccgctgctga aatcgcaggg gaacgccgtc atcacgaagc tccccatgat
      721 cgggacgaag gagcatgtgc tgagcaaggg tgtcaagaag acgctgctcg aagtcgagtt
      781 cgacgatttc acgctcttcc tgacgcatct tcccttgggg tacgccgcac ggtccgtgca
      841 gctcggcgag atcgcggagc gctgcatcga cgcgaagaaa ccggttatcc tggccggcga
      901 cggcaatacc tacagaggtg agcgggaact gcgctcgttc ttccggaaga ccggttttca
      961 aaacgccaat ccgacgaaca cgccgacatt ccccagcagg attccggcgc tcgcgctcga
     1021 ctttgttctc tacggtccgg ggatcgagat ggagcgtttc gacgtgccgc gggtccgtta
     1081 ttcggaccat ctgccgctgg tgtgcgactt cacggtcgcc cgccctaaaa aggagcgaca
     1141 ctctcaatga ccgtcacgat gctcttgtat tcggcctatg aactttcttc gatcgccatc
     1201 cgcctcatca tgctctgcta cattccgctg aagcacaccc cctccacggc gaccgcatgg
     1261 ctgctcgcca tctacttctg gccgtggctg ggcttcctgc tgtactctct cttcggctcg
     1321 accagcctgc cccaagagcg aatcgaccgc catgaaacga tgcttcgcag cctcgacgag
     1381 gacaaacgga tgttccgccg catcttcgac gaagaagccc actggcttcc ggacgaactt
     1441 gtgcgcttcg gcgcgctcgg cagaaagctc ggcgacatgt gcatcacccg ggggaacgac
     1501 ttcctcctta tcgaccggga agaggctttc atccgctcgc ttgccgccga tgtggagaac
     1561 gcgcagcgct ccgtccacct gcttttctac atctttgccc gcgatgcggt aaccgagcct
     1621 ctcttccgcg cgcttgaagg cgcggcggcg cggggcgttg cgtgccgcgt actcgtcgac
     1681 gcgctcgggg ccaagccatt cctgaagcgc gacgccgaac gccttcgagc agcgggaatc
     1741 agggtggaga aggcgctgcc tcctcggctg cttcgccgga cgccgaccac cgcccgctac
     1801 gacctccgca accaccggaa gatcgccgtg atcgacggcc gcgtagggta caccgggtcg
     1861 cacaacgtca cggagccttc ctacggcgga aaggcgaagg ggctgctctg gcgcgacctt
     1921 acgctccggc tcgtcggccc cgtggtgttg cagcttcagg gggtcttcat ggaggactgg
     1981 ttcgtcgaga cgcgggagat gctcctctcc gacgaggcgt ttccggctcc cgaatacgcg
     2041 ggcgaggctg tcgttcagac ggttccgagc gggccgtcct atccgtggca gaactaccag
     2101 cggcttgtcg tctcggcgct ccacggggcg aagaagcgcg tggtcatcac gacgccgtac
     2161 ctcattcccg acgacgaact gcttcacgcg ctggagacag c
//
LOCUS       DAOPKU010000513         2198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_57907_length_2198_cov_5.023798, whole genome shotgun sequence.
ACCESSION   DAOPKU010000513 DAOPKU010000000
VERSION     DAOPKU010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57907_length_2198_cov_5.023798"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PK849_12955"
     CDS             complement(<1..1004)
                     /locus_tag="PK849_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPE67082.1"
                     /translation="MANKMITAVINGKTITAESGSTILQAARANNIYIPTLCDHPSLP
                     PSGACRVCLVEAERNPKLLTACTTPLTEGMVVSTNSPRVLSARKAVVEMLLIRHPLDC
                     FSCESNGRCELQNLAYELGIEKSPFTDEGDVCTHFELDNTNPFFIRDMNKCILCGRCV
                     RACDWRAGYHAIDFQFRGINTQINPPIGTKLEDEASDCVFCGQCVQVCPVGALVEKKS
                     VGQGRPWQTHVVRTTCPYCGVGCQLDLHMNGEQIARVTGTEDGMPNLGRLCVKGRFGY
                     DFIYSDERLTKPLIRERKGEKKGSDDTFREASWDEALDLVASRLKEVMQKHGPDAVGG
                     V"
     gene            complement(1032..2048)
                     /locus_tag="PK849_12960"
     CDS             complement(1032..2048)
                     /locus_tag="PK849_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPE67083.1"
                     /translation="MEREIVPVELAVGRAIAHDLTLIDPESGFKGAKFRRGHTIRPED
                     VPVLQRMGKSNISFLRMAPGEVHEDDAALRIGRRVAGDGVALRGPEEGKCSLVAERDG
                     LLVYRDEDIDFVNDDPDWLFTTLPNKVRVGRGKTVAAFRIGPLMVREEQVARVESIGP
                     LSVLPWRRLKTALVTTGRELYEGIVKDAFQPKLMKKLAGYGGEFLGGRVVPDDRSAIQ
                     EAISGWLDEGAELVLCTGGMSVDADDMTPSAIRAVCDSVLFRRTPVIPGANLMLAAKD
                     GAFVLGVPASAVFIERTSLDILLDRLFAGVPPDGKEVRGWGRGGLCAHCDSCGYPRCA
                     FAAR"
     regulatory      complement(2132..>2198)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
BASE COUNT          383 a          718 c          664 g          433 t
ORIGIN      
        1 actcctccca ccgcgtccgg gccgtgcttc tgcatcacct ccttcagtcg ggatgcgacg
       61 aggtcgagcg cctcgtccca ggaagcctcg cggaacgtat cgtcggaccc cttcttctcg
      121 cccttgcgct cgcggatgag gggcttcgta agccgttcgt cggagtagat gaagtcgtag
      181 ccgaagcgtc ccttgacgca gagacgcccg aggttcggca tgccgtcctc cgtgccggtg
      241 accctggcga tctgctcgcc gttcatgtgc aggtcgagct ggcagccgac gccgcagtac
      301 gggcacgtcg tgcggacgac atgcgtctgc cagggcctgc cctgcccaac cgacttcttc
      361 tcgacgagcg ccccgaccgg acagacctgg acgcactgcc cgcagaagac gcagtcggag
      421 gcttcgtcct caagcttcgt ccctatgggc gggttgatct gcgtgttaat ccctctaaac
      481 tggaagtcga tggcgtggta accggctctc cagtcgcagg cgcggacgca gcggccgcag
      541 aggatacact tgttcatgtc gcggatgaag aaggggttcg tattgtccag ttcaaaatgc
      601 gtacagacgt cgccctcgtc ggtgaagggg gatttttcga tgccgagttc gtaggcgagg
      661 ttctgcagtt cgcacctgcc gttggactcg caggagaagc agtcgagcgg atggcggatg
      721 agcagcatct cgacgacggc tttgcgcgcc gaaagaaccc tggggctgtt cgtggacacg
      781 accatgccct cggtaagcgg cgtcgtgcag gcagtgagga gtttcgggtt tctctccgcc
      841 tcgacaagac agacgcggca cgcgccgctc ggaggcagag acggatgatc gcagagcgtc
      901 gggatataaa tgttgttggc ccgggccgcc tggaggatcg tggacccgga ctccgccgtg
      961 atcgtttttc cgttaatcac agcggtgatc attttgttcg ccatggttca gtctcctttt
     1021 cttttttctc gctatcgcgc cgcgaaggcg catcgcggat agccacagga gtcgcagtga
     1081 gcgcacagtc ccccccttcc ccagcctctg acctctttgc cgtcgggcgg aacgcccgcg
     1141 aagagcctgt caaggaggat gtcgagcgac gtcctttcta tgaacacagc cgatgccgga
     1201 acgccgagga caaacgcgcc gtccttggcg gcaagcatga gattggcccc gggaatcacc
     1261 ggcgttcttc gaaacagtac gctgtcgcac actgcgcgga tcgcggaggg cgtcatgtcg
     1321 tcggcgtcca cgctcattcc gccggtacag agcacaagct ccgcgccttc gtccagccag
     1381 ccggagatcg cctcctggat ggctgaacgg tcgtccggga cgactcggcc gccgagaaac
     1441 tctccgccgt acccggcgag ctttttcatc agcttgggtt ggaacgcgtc cttgacgatt
     1501 ccctcgtaca gctcgcgccc cgtggtcacg agggcggttt tcagacggcg ccacggaagc
     1561 accgatagag gccctatgct ttcaacgcgg gcgacctgct cctcccgtac catcagcggc
     1621 cctattctga aagcggcgac ggtctttccc ctcccgacgc gcactttgtt cggcagcgtt
     1681 gtgaaaagcc agtcgggatc gtcgttgaca aagtcgatat cctcgtcgcg atagacgagc
     1741 agtccgtcgc gctccgcgac gagcgagcac ttcccctcct cgggaccgcg cagcgcgacg
     1801 ccatccccgg caacgcgccg tccgatgcgg agcgccgcgt cgtcctcgtg gacttctccg
     1861 ggggccatcc gaaggaacga gatgttcgac ttccccatgc gctgcagcac gggaacgtcc
     1921 tcgggccgga tcgtatgccc ccggcggaac ttcgctccct tgaaaccgct ctccgggtcg
     1981 atgagcgtca ggtcgtgcgc gattgccctg cccaccgcca gctcaaccgg aactatttct
     2041 ctctccattc gttctcattc cttcgctcaa gcattgaaaa aacacaacgg ggaccgccgg
     2101 tcagcaaaga cgacggtccc cgtagagtca ctctcgggtg cttcgtcctc cttgccaaac
     2161 acgggaggca tgcccgtttc caggcatacc ctgacggc
//
LOCUS       DAOPKU010000514         2198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_57908_length_2198_cov_4.901540, whole genome shotgun sequence.
ACCESSION   DAOPKU010000514 DAOPKU010000000
VERSION     DAOPKU010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57908_length_2198_cov_4.901540"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..816
                     /locus_tag="PK849_12965"
     CDS             28..816
                     /locus_tag="PK849_12965"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPE67084.1"
                     /translation="MLSYGPIVPANRFRKAGIALGVVLLVCCCFSAGCAWAGSPLEGA
                     RQLVVVVAGDWSSVPAVLQRYERGTETASWRPVGKRIQVSLGRSGQGWGIGLHPEEAR
                     RSGEPEKREGDGRAPTGVFELVSCFAYAPEELAGTAMLVLKADSELVCVDDSSSRYYN
                     EVFPGNVPDKDWNSAEDMLRKDGLYRYGVVVAHNRNPPLPGRGSCIFLHVERSPGAPT
                     AGCTAMKQSAVKELILWLDPAAKPMLAQFPKDVYERLKEEWAMP"
     gene            1041..1598
                     /locus_tag="PK849_12970"
     CDS             1041..1598
                     /locus_tag="PK849_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67085.1"
                     /translation="MLITSVLFRRKLVPLFALFVLLFGVFPANAELNRITEAEILDAQ
                     KGWAEGVVSIGRAYVDKKDYKAAAAEFVDAFYGYDQGPVLFKPTKASAVKFRLTRGEA
                     LSYFVKGSVPEDHGFVLQPWSNVRFENAGIVIDGDSALAMGVYFFTDANTSEEVEVEY
                     TFGYFKDESGKIRIRLHHSSVPYSE"
     gene            complement(1719..>2198)
                     /locus_tag="PK849_12975"
     CDS             complement(1719..>2198)
                     /locus_tag="PK849_12975"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPE67086.1"
                     /translation="EMALEELEQLEKYQGPDECILAHVAPMRVRSAPLAGERLRLREI
                     ARSEAKHIFGEDGSSSPLLCVYDLQTGPASATICIPAEEPDGEIMPGCVLAVSLHRGA
                     YETIPATLEQLLAWVKRNQYRTNGKVVERYFPCGEEDHPHGRVEIAVALDDDSHTSQ"
BASE COUNT          439 a          572 c          713 g          474 t
ORIGIN      
        1 aggttttgta agagaaggag gttcgctatg ctgagctacg gaccgattgt tccagcgaat
       61 cgctttagaa aggccgggat tgctctcgga gtggtcctgc tcgtttgctg ttgtttctcc
      121 gcagggtgcg cctgggcggg ctcgcctctt gaaggagccc ggcagcttgt ggttgtggtt
      181 gccggcgact ggagcagcgt gcccgccgtg ctgcagcgct acgagcgcgg cacggagacc
      241 gcttcgtggc ggccggtcgg gaaacggatt caggtcagcc tggggcgcag cggccagggg
      301 tggggaatcg ggctgcatcc ggaggaggcg agaaggtccg gcgaaccgga gaagcgcgag
      361 ggcgacggcc gggccccgac tggggtgttc gagctcgttt cctgtttcgc ctacgctccg
      421 gaggaactcg ccggaacggc gatgctcgtc ctgaaggcgg attcggagct ggtgtgcgtg
      481 gatgattcct catcccgcta ttacaacgag gtgtttcccg gaaatgtccc ggacaaggac
      541 tggaacagcg cggaagacat gctccgcaag gacgggctgt accgctacgg cgtcgttgtg
      601 gcgcacaacc ggaatccccc gcttccgggc agaggatcgt gcatcttcct ccatgtggag
      661 cgctctcccg gcgcgccgac ggcggggtgc acggcgatga agcagagtgc ggtgaaggag
      721 ctgatcctgt ggctcgaccc cgcggcgaag ccgatgctgg cgcagttccc gaaggatgtg
      781 tacgagcgcc tgaaggaaga gtgggcgatg ccgtagggcg gggtttttct ctgtaggagc
      841 cttgggtgaa gagcggaaaa agaataagaa aatttactga aataaaaaat tccgaaaagt
      901 gcgcttgaca gtcatactcc agtatggtat aaaatttata ccataagagc ttgcggctct
      961 tcttgcatcg ttacatccag aaacgggtcc ggcgaatgcg accggacgat gaagaggtgg
     1021 agtacgagga ggtgttccgt atgttgataa cgagcgtgct tttccggagg aaactggttc
     1081 ccctgtttgc gctgtttgtt cttttattcg gcgtgtttcc tgcaaatgct gaactgaatc
     1141 gaatcaccga ggcggagatc cttgatgcgc aaaaaggatg ggcggaaggc gtcgtctcca
     1201 taggcagagc ctacgtcgac aagaaagact acaaagccgc ggcggcggaa ttcgtcgatg
     1261 cgttttacgg gtacgaccaa ggccctgtac tgttcaagcc gacgaaggct tcggcggtga
     1321 agttccgttt gacgagagga gaggcgctct cctatttcgt gaagggaagc gtccccgagg
     1381 atcacggatt cgttcttcag ccgtggagca acgtgcgctt tgagaacgcc ggaatcgtga
     1441 tcgacggcga ctccgccctg gcgatgggcg tctatttttt taccgacgcg aacacatccg
     1501 aagaggtgga agtcgaatat actttcgggt atttcaagga tgagagcgga aagattcgta
     1561 tcagactcca tcattcctcg gtaccgtatt cggaatagag caggaaaaac gattcgggag
     1621 atagcataac gaatggccgg agaggatgcg agtccgctcc ggccattcgt ttcgtcttca
     1681 ggtgagaatc acccgtttgg ttgatgtatt tgtaccgtct actgcgaggt atgagagtcg
     1741 tcgtccagcg cgacggcgat ctcgaccctg ccgtggggat gatcttcttc tccgcagggg
     1801 aagtaccgct cgacaacttt cccgttcgtc ctgtattggt ttcgtttcac ccacgcaaga
     1861 agctgctcca gggtggccgg aatcgtctcg taggcgcctc tgtggagcga gacggccagc
     1921 acgcatcccg gcatgatctc tccgtcgggc tcttccgccg ggatgcagat cgtcgcactg
     1981 gccggacctg tttgcaggtc gtagacgcac aaaagaggcg agctgctgcc gtcctctccg
     2041 aagatgtgct ttgcctcgga gcgtgcgatc tcgcgaaggc gcaaccgttc tccggccaac
     2101 ggggcggaac gtacgcgcat cggggcgaca tgcgccagta tgcactcatc cggcccctgg
     2161 tatttttcga gctgttccag ttcctccagt gccatctc
//
LOCUS       DAOPKU010000515         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_58029_length_2195_cov_4.689720, whole genome shotgun sequence.
ACCESSION   DAOPKU010000515 DAOPKU010000000
VERSION     DAOPKU010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58029_length_2195_cov_4.689720"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..230
                     /locus_tag="PK849_12980"
     tRNA            145..230
                     /locus_tag="PK849_12980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:179..181,aa:Ser,seq:tga)
     gene            complement(327..1091)
                     /locus_tag="PK849_12985"
     CDS             complement(327..1091)
                     /locus_tag="PK849_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPE67087.1"
                     /translation="MEKTFVRCIIRGEERQALVADGAVYALRAPMGDGAEPGERLGTL
                     DEVERFLPPTSPTKVVAVGLNYRDHAEEVGLPIPEEPLIFIKPSTSVVGHGDPVVYPD
                     VMTKRVDYEAELGIVIGKRCRSVSPEDALGYVLGYTCANDVTARDLQSKDGQWTRSKS
                     FDTFCPLGPFVVSGVDPSDLEITMLVNGKVVQHSRTSNFIFPVPVLVSHISHVMTLEP
                     GDVIITGTPAGIGPVKKGDLMRVEIERLGALENRVE"
     gene            1357..2103
                     /gene="phoU"
                     /locus_tag="PK849_12990"
     CDS             1357..2103
                     /gene="phoU"
                     /locus_tag="PK849_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPE67088.1"
                     /translation="MFGKKNGKVESLLYSGDREKILAMVREMGSMASEAFRLAVQSLE
                     ERDDALAERVIEEDDAIDDLEATIDHECLCSIAMRQPVREDLRFVFAVLKIITDLERI
                     GDQGVNIAKKARVLNRYPLLKPLVDIPKMKVIATEMVADSLKAFEENDTLLAEEICMR
                     DEELDKLYDCIFDELIGILGKKPEGDQATAQCAAGLLWVARHLARIGDHATNVAERVF
                     FMVKGERLKPLIEAKKKALQEKIEENGFPH"
BASE COUNT          501 a          627 c          617 g          450 t
ORIGIN      
        1 tcacctctcc cgaagaatcg ttgccgcacg cactctcttt gagcacaata atattatacc
       61 ctacttggta gaagcgcaaa aaaggctttt ctcacgcagc acgttggcaa gagtacaaaa
      121 gatgtgtata atgctcttca tgctggaggg ctgtccgagc ggtcgatggt ggttgacttg
      181 aaatcaactg ggcgtaagcc ccaggggttc gaatcctctg ccctccgcca ttcacatgca
      241 tgatcggacg tttttacgtc cgcagacgaa aagggggaac gcgagcggaa cgcggtcccc
      301 ctttttgcgt atgcttccga atcaggctac tcgacccggt tctccagcgc gcccagacgt
      361 tcgatctcga cgcgcatcag atcccccttc ttcaccggcc cgattccagc cggggtgcct
      421 gtgatgatca catcgccggg ttccagcgtc atcacgtgcg agatgtggct gacgagcacc
      481 ggcacgggga agatgaagtt gctcgtgcgg gagtgctgga cgaccttgcc gttcaccagc
      541 attgtaattt ccaggtccga cggatcgacc ccggaaacaa cgaacggccc gagcggacag
      601 aacgtatcga aggacttcga ccgcgtccac tgcccgtcct tcgactgaag atccctcgcg
      661 gtcacgtcgt tcgcgcaggt gtaccccaaa acgtagccga gcgcatcctc cggcgacacg
      721 ctcctgcacc tcttcccgat gacgatgccg agttcggcct cgtaatccac ccgtttggtc
      781 ataacgtccg gatacacgac cggatcgccg tgccccacga cgctcgtcga gggtttgatg
      841 aagatgagcg gctcctcggg tatcggaagc cccacctcct cggcatgatc cctgtagttc
      901 aacccgaccg cgacgacctt cgtgggcgaa gtcggaggga gaaaacgctc gacctcgtca
      961 agggttccga ggcgctctcc cggctctgct ccgtctccca tgggagcgcg cagagcatac
     1021 actgcgccgt cggcaaccag cgcctgccgc tcctctccgc ggatgatgca tcgtacaaaa
     1081 gtcttttcca tagtccctct ctcctccggg aaaaaagtct ctacatcttg aaatgtataa
     1141 tagcagattc gccgcgcaga aaacgaattc ccgtgaaaaa aacgttgaac gggagcgcac
     1201 ggccgcttca ttataattga ctttccggaa ggcatatcgt atcctgtaac attatcgtta
     1261 caaaaacatt gccccggttg acgcttcccg tacgagtcct tctccgacaa gggaaataca
     1321 acggggccgt tgatgtattt cgaggagggg tggagtgtgt tcggaaagaa gaacgggaag
     1381 gtcgaatccc ttctctattc aggagacaga gagaagattc tcgccatggt ccgcgagatg
     1441 ggcagtatgg cgagcgaggc gttcagactc gctgtccagt cgctcgaaga gcgcgacgac
     1501 gcgcttgcgg agagggttat cgaagaagac gacgcaatag acgacctgga ggcgacgatc
     1561 gaccatgagt gtttgtgctc gatcgccatg cgtcagcctg tccgcgagga tctccgcttc
     1621 gtcttcgcgg ttttgaagat catcaccgac ctggagcgga tcggcgacca gggggtcaac
     1681 atcgcgaaga aggcgagggt gctcaaccgg taccctctgt tgaagccgct cgtggacata
     1741 ccgaagatga aagtcattgc gacggaaatg gtggccgact cgctcaaggc ctttgaggag
     1801 aacgatacgc ttctcgccga agagatctgc atgagggacg aggagctgga caagctgtac
     1861 gactgcatat tcgacgaact catcggtatc ctggggaaaa aaccggaggg cgaccaggcc
     1921 actgcgcaat gcgccgcagg tttgctctgg gtcgcgcgcc atctcgcccg tatcggcgac
     1981 cacgcgacca atgttgcgga gagagtcttt ttcatggtga aaggagaacg gctgaagccc
     2041 ctcatcgaag caaagaaaaa ggcccttcag gagaagatcg aggagaacgg attccctcac
     2101 tgaggccttc ccgtttttgc ctttgattga aaggaggtgg aacgatttcc cctctgtcaa
     2161 gcgctataga ttcaacagat gcaccacaca tttga
//
LOCUS       DAOPKU010000516         2190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_58214_length_2190_cov_3.016862, whole genome shotgun sequence.
ACCESSION   DAOPKU010000516 DAOPKU010000000
VERSION     DAOPKU010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58214_length_2190_cov_3.016862"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PK849_12995"
     CDS             complement(<1..791)
                     /locus_tag="PK849_12995"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HPE67089.1"
                     /translation="MNEVFSMWKKTKMIVLVALCAGLYAALLIPFKGFVLIPGVTEFR
                     PASALPVVMGLLFGPAGAWGSAIGNLIGDFFGSLGIGSLFGFVGNFMFAYVPYKLWIN
                     LGLVSKQDKEPDLKSKRKIAAYVVVAILGSVACALVIAWGLEVLKLVPFAALASIISL
                     NNSLPSVVLGIPVLLVLYPRIKKWDLLWTDIMDEEDIPVAGGMSRIGGFFMTVSILGG
                     MIGGLMAAFGAGQAFLYKGFAAGGDVGSLGVLLVAGIGSVGLVLSS"
     gene            complement(886..1677)
                     /locus_tag="PK849_13000"
     CDS             complement(886..1677)
                     /locus_tag="PK849_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPE67090.1"
                     /translation="MDMFLYIDKETPVHRLDPRTKLLLMLLSFVLALSFRNFAILSSV
                     LVGVLLYGAAGGALSNLRRIRVVLLMIALFSMGIWSVATLSDSRFLIFSLEGLLYGVM
                     VAIKTDLMIISGMIFLSTTKIEEIAGGLVKLKLPYRGAFAFSTAIRLVPMIVATSYTI
                     IQAQKSRGLDLDSGSMLQRARKYVPLVIPTLVSVIRGTNVFSMALESKGFGYSDTRTN
                     LLELRMKRNDWLTLGVALLVVAAAFFFNMDSVAYGACLFSNLAGS"
     gene            complement(1677..>2190)
                     /locus_tag="PK849_13005"
     CDS             complement(1677..>2190)
                     /locus_tag="PK849_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE67091.1"
                     /translation="FEEVAFSPKIRGCPEEELAVRVREALEAVDLAGYEKKDPFSLSK
                     GERQRVAVASVLSARPGIIILDEPTTGLDYTEQRKMMELVRKLNESGQTVIMITHTMW
                     VVAEYAHRVVVVKDGRVLLDGATRDVFAREPELKASHLRPPHIVSMSNLLGHTLLSVE
                     EMKYCTGGVS"
BASE COUNT          473 a          762 c          597 g          358 t
ORIGIN      
        1 gacgaaagca ccagccccac cgaaccgatt cccgcgacca ggagcacgcc gagcgaaccg
       61 acgtcgcctc ccgcggcgaa ccccttgtac agaaacgcct ggcccgcgcc gaacgccgcc
      121 ataagcccgc ctatcatccc tccgagaatg ctcaccgtca tgaagaaacc gccgatcctg
      181 gacatgccgc ccgctacagg aatgtcctct tcatccataa tgtcggtcca gaggagatcc
      241 cacttcttga tccgggggta gagcaccaga agcacgggaa tacccagtac aacggagggg
      301 agggagttgt tcagcgagat gatgctcgcg agcgccgcga acggcaccag tttgagcacc
      361 tcgagccccc atgcgatcac gagcgcgcag gcgaccgacc ccagaatcgc gacgacgacg
      421 tacgcggcga tcttccgctt cgacttcagg tcgggctcct tgtcctgctt cgagaccagc
      481 cccaggttga tccagagctt gtacggcacg tacgcaaaca tgaagttccc cacgaagccg
      541 aaaagactgc cgatacccag cgagccgaaa aagtcgccga tgagattgcc tatcgccgac
      601 ccccacgccc cggcggggcc gaacagaagc cccatcacca cgggaagggc gctcgccggg
      661 cggaactccg taacccccgg aatgagcacg aaccctttga aagggatcag cagggctgcg
      721 tacaggccgg cgcagagcgc cacaagcact atcatcttcg ttttcttcca catcgagaat
      781 acttcgttca caaagaccac tccttcaagg tttgactccg cgtttcgcca tatccgccgc
      841 taaggccgcg gcgctccgcc ctcgctccgc agcgatgggc gcgcttcagc tccccgcaag
      901 gttgctgaaa agacacgctc cgtacgctac cgaatccata ttgaagaaaa acgccgccgc
      961 gacgacaagc agcgcgacgc ccagcgtcag ccagtcgttc cgcttcatcc gcagttcgag
     1021 aagattcgtc cgtgtgtcgc tgtacccgaa ccccttcgac tccagcgcca tcgagaagac
     1081 gttcgtcccc cggatcaccg aaaccagcgt gggaatgacc agcggcacgt acttgcgcgc
     1141 ccgctggagc atcgatcccg aatcaaggtc aagcccgcgc gacttctgcg cctggatgat
     1201 cgtgtagctt gtggccacga tcatcggcac aaggcggatc gcggtggaga acgcgaacgc
     1261 cccccggtac ggcagcttca gcttcaccag acccccggcg atctcttcga tcttcgtcgt
     1321 gctcaggaag atcatccccg agatgatcat caaatcggtc ttgatcgcca ccatcacgcc
     1381 gtagagcagc ccttcgaggg agaagatcag aaaacgggag tccgagaggg tggcgaccga
     1441 ccagataccc atggaaaaga gcgcgatcat cagcagcacg acgcgtatcc gccgcaggtt
     1501 ggagagcgcg ccccccgccg cgccgtagag cagcacgccg acgagcaccg acgacagaat
     1561 agcgaagttc ctgaacgaga gcgccaggac gaaactgagg agcataagca gcagcttcgt
     1621 ccgcgggtcc agccggtgaa cgggcgtctc tttgtcgatg tagaggaaca tatccatcac
     1681 gaaacgcctc ccgtgcagta cttcatctcc tccaccgaga gcagcgtatg cccgagcaga
     1741 ttgctcatcg agacgatgtg cggcggccgc aggtgcgacg ccttcagctc cggctccctg
     1801 gcgaagacgt cgcgggtcgc cccgtcgagc agcacccgcc cgtcctttac cacgaccacc
     1861 ctgtgggcgt actccgccac cacccacatc gtatgcgtga tcatgatcac cgtctgcccc
     1921 gactcgttca gcttccttac caactccatc atcttccgct gctccgtgta gtcgagccct
     1981 gtggtcggtt cgtcgaggat gatgattccc ggacgcgccg aaagcacgga ggccacggcc
     2041 acacgctgcc gctctccctt gctcagcgag aaggggtcct tcttctcgta tcccgcgagg
     2101 tccaccgctt cgagcgcctc gcgcaccctg acggcgagct cctcctccgg acaccccctg
     2161 atcttcggac tgaacgcaac ctcttcgaag
//
LOCUS       DAOPKU010000517         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_58237_length_2189_cov_4.265230, whole genome shotgun sequence.
ACCESSION   DAOPKU010000517 DAOPKU010000000
VERSION     DAOPKU010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58237_length_2189_cov_4.265230"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..112
                     /locus_tag="PK849_13010"
     CDS             11..112
                     /locus_tag="PK849_13010"
                     /inference="COORDINATES: protein motif:HMM:NF018476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HPE67092.1"
                     /translation="MAGFWSRRIIGEHRILYRVEDDALLIPQLRFHY"
     gene            complement(172..570)
                     /locus_tag="PK849_13015"
     CDS             complement(172..570)
                     /locus_tag="PK849_13015"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HPE67093.1"
                     /translation="MSELVKSEGGSLVGLFHRKGGDLAIGKPFERDIFLFATHVAGTS
                     YVEGIEELEPHLNVGDTLDFFREPDNPYDKRAIVIRNKDGVKIGYVPKADNVVFSRLM
                     DAGKLLFGRISAKQTQGNWLKIDIKVFLHE"
     gene            complement(575..1420)
                     /locus_tag="PK849_13020"
     CDS             complement(575..1420)
                     /locus_tag="PK849_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67094.1"
                     /translation="MKHSLEPNGERPDTTDAAVIMLSQDFERLKSEVEKLRTELSMLV
                     LERDELLLVECKNIETAYMLSIGALEYKAYEIECAVLRMKRKAELIQAKKNRQEKVVL
                     SKIEELLDIAFAEYQAKLNERIEKMNAAIERSHGRLLSDEETRELKSLYRAVVKALHP
                     DLHPDSSDAKMQLFQNAVTAYGNGDLDGLRIIAAMVAEPAPLYDGPDATAQLTKEKER
                     LSTLLRKVKDRIAEIKSEYPYTMKPIVKSPEKTEARKVELQEYIARLRETLAAYTAKI
                     DEMLR"
     gene            complement(1570..1827)
                     /locus_tag="PK849_13025"
     CDS             complement(1570..1827)
                     /locus_tag="PK849_13025"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPE67095.1"
                     /translation="MVNLWKVNLSPEAKKRLQKIPNPDRRRILTALDALYTGPSGDIK
                     PLKGRVEWRLRVGGWRVLLLIDENTATIRVVSIDSRGDVYK"
     gene            complement(1821..>2189)
                     /locus_tag="PK849_13030"
     CDS             complement(1821..>2189)
                     /locus_tag="PK849_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67096.1"
                     /translation="RERIPLNLPDQPVDPLDHLPVLFFHTLPVFSRKCYYYFIIKEGG
                     LPMTNLIKERNEVRRVLRRLSPRGVERLRTYALHVEMEERQDLEPPLTPEEEECLAIS
                     RAEFAEGKEIPLKDVMDELW"
BASE COUNT          405 a          628 c          601 g          555 t
ORIGIN      
        1 gaagcatgct ctggccggtt tttggtcgcg caggataatc ggagaacacc gtatcctcta
       61 tcgcgtcgag gacgatgcgc ttttgatccc gcagcttcgc ttccattact gaaacgggtc
      121 cgacggagcc gagaaccgct tcgttggctc gctgctccca ttgcacggcg cttactcgtg
      181 caggaagacc ttgatgtcga ttttcagcca gtttccttgc gtctgcttgg cggagatcct
      241 tccgaagagc agtttgcccg cgtccatgag gcgcgagaaa accacattgt cggccttcgg
      301 gacatagccg attttgacgc cgtctttatt cctgatgacg atcgccctct tatcgtacgg
      361 attgtcgggc tcgcggaaga agtccagcgt gtcgccgacg ttgaggtgcg gctccagttc
      421 ctcgattccc tctacgtagc ttgttcctgc aacgtgcgtg gcgaagagaa atatatcccg
      481 ttcgaacggc ttgccgatag ccaggtcgcc gcctttgcgg tgaaacaggc cgacaaggct
      541 gccgccttcg ctctttacca gctccgacat gtgctcacct cagcatttcg tcgatcttcg
      601 ccgtgtacgc ggcaagcgtc tcgcgcaacc gggcgatgta ctcttgaagc tcgaccttgc
      661 gggcttccgt cttttcggga cttttgacga tcggcttcat cgtgtagggg tactccgact
      721 tgatctcggc tatcctgtct ttcacctttc gcagcagcgt ggacaggcgt tctttctcct
      781 tggtcaactg tgctgttgca tccggtccat cgtagagcgg cgccggttcg gcgaccatcg
      841 cggcgatgat gcgaagcccg tccaagtcgc cgtttccgta cgcggtcacg gcattttgga
      901 aaagctgcat cttcgcgtca ctcgaatccg ggtgaaggtc cggatgcagc gctttcacga
      961 cggcgcggta caggctcttg agttcgcggg tttcctcgtc ggagagcaat ctgccatggc
     1021 ttcgctcgat ggccgcgttc attttttcga tccgctcgtt cagcttcgcc tgatattcgg
     1081 cgaacgcgat atcgagaagc tcttcgattt tggagaggac gactttctcc tgccggttct
     1141 ttttcgcctg tatcaactcg gcctttctct tcatgcgcag aacggcgcac tcgatctcgt
     1201 acgccttgta ttcgagggcg ccgatggaga gcatatacgc cgtttcgatg tttttgcact
     1261 cgacgagcag aagctcgtcc cgttcaagga caagcatgga gagttccgta cggagctttt
     1321 ccacctcgga cttcagtctt tcgaagtcct gagagagcat aatgacggcg gcgtccgtgg
     1381 tatcagggcg ttcgccgttc ggctcaagag agtgtttcaa ggcatatccc ccatcctccg
     1441 cagcccggaa gagagttgtt gacggcgttg acgatggcga ccacatgcag cgtggtgatg
     1501 tccgccttga tgacggtcag catggcgcta ttctccgacc gtgtccgcac ctttttggaa
     1561 gatggggcat catttgtaca cgtctcccct ggaatctata gatactactc gaatcgtcgc
     1621 cgtattttca tcgatcagca acaaaaccct ccagcctccg actctgagtc tccattcaac
     1681 acggcccttc agcggcttga tgtcgccgga agggccggtg tacagcgcat cgagcgcagt
     1741 caatattcgt cgtcggtcgg ggttcgggat tttttgcaag cgtttcttcg cctccgggga
     1801 gaggttgact ttccagagat tcaccatagt tcatccatga cgtccttcag cggaatctct
     1861 tttccttcgg cgaactcggc tctcgaaatc gccagacact cctcttcctc cggagtcagg
     1921 ggaggctcca aatcctgccg ctcctccatt tcgacgtgga gcgcatacgt ccgcagacgt
     1981 tccacccctc gcggggaaag tcgccgaaga acgcggcgta cttcgttgcg ctctttgata
     2041 agattggtca ttggcaatcc cccttctttt atgatgaaat aatagtagca cttccgggaa
     2101 aaaactggaa gtgtatggaa aaacaggaca ggcagatggt cgagcggatc gacaggctga
     2161 tcagggagat tcagcgggat ccgttcgcg
//
LOCUS       DAOPKU010000518         2173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_58843_length_2173_cov_4.593484, whole genome shotgun sequence.
ACCESSION   DAOPKU010000518 DAOPKU010000000
VERSION     DAOPKU010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58843_length_2173_cov_4.593484"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PK849_13035"
     CDS             <1..487
                     /locus_tag="PK849_13035"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPE67097.1"
                     /translation="ASAIGARMNLDAERCEGLRIAGLVHDVGKIEIAGEILNKPGRLS
                     PIEFELVKTHSQSGYEILSGIDFPWPVAEIALQHHERLNGSGYPRGLKGNEILLEARI
                     IAVADVVESMISHRPYRPALGMDAAREELKARKGEFYDPEVVDACLGLLEERPDIIGA
                     E"
     gene            complement(495..1790)
                     /locus_tag="PK849_13040"
     CDS             complement(495..1790)
                     /locus_tag="PK849_13040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE67098.1"
                     /translation="MRHASPSSRDEARGISSRRAHFLFLCLVGAYFFSFFFRISASVV
                     LPSQAAKLGMSAALTGFISSLYYYAYSVMQSISGALHDRFGPLRVVAGGMLVAAAGTF
                     LLVLHPSEWTLGAWRLLTGFGLAPMYGGALVYQASAFTPDKYVFYSSITISVGAFGAL
                     ASVAPLGLALDAFGLSATFAGLTLLSISMSVLLWKERSFDPVVHRTPFTEQKTQSILS
                     RLAEALRLIRSSAYLRSLLVLWTVSAGALLSFQGLWAVSWYQTAYGVAAATARNWASL
                     VSVGMFIGTAVFGGFCRRPEDRFRGVCFWTLFNVASWGALVSSVALKLPLPVAGTCGF
                     LIGAANGLIAVQASSAAKEQVETAKTGALLGTMNMIVIFVVVLFQWGTGILINLFPGG
                     QAGEFTERGFLVCFLIVVVLLAGSLPALPPLRRADTRRR"
BASE COUNT          517 a          580 c          690 g          386 t
ORIGIN      
        1 ggcttcggcg atcggcgcca ggatgaacct tgatgcggag cgctgcgagg ggcttcgtat
       61 tgcaggtctc gtgcatgacg tcggcaagat tgagatagcc ggtgagatat tgaacaaacc
      121 cgggcgtctt tcacctattg agttcgagct tgtgaagacg cattcccaat cgggatatga
      181 aatcctgagc ggaatcgact tcccctggcc tgtagccgag atcgcgctgc aacatcatga
      241 acggctgaac ggctcgggct atccccgggg actcaagggg aacgagatcc ttctggaagc
      301 ccgtattata gcggtcgcgg atgtcgtgga gtcgatgatt tcgcaccgcc cctaccgtcc
      361 cgcgctgggg atggatgcgg cgcgggagga actcaaggcg cgaaaggggg agttctacga
      421 ccccgaagtt gtcgacgcgt gtcttggtct tcttgaagag cggccggata tcatcggcgc
      481 agagtagagg cgctctatcg gcgtcgtgtg tcggctcgcc ggagcggcgg gagcgcgggc
      541 agactgccgg ccagcagcac tacaacaatc aggaagcaga cgaggaaacc tcgttcggta
      601 aactcccccg cttgccctcc gggaaagaga ttgatgagta ttcccgttcc ccactggaag
      661 aggaccacaa caaagatgac gatcatgttc atcgttccaa gaagagctcc cgttttagcc
      721 gtttccacct gttctttcgc cgccgacgag gcctggacgg caatgagccc gtttgctgcg
      781 cctatgagaa atccgcatgt cccggcgacg ggaagcggca gtttcagcgc tacggaactc
      841 actaacgcac cccaggacgc cacgttgaag agagtccaga aacacacccc ccgaaaacga
      901 tcctccggac gccggcaaaa cccgccgaaa acggcggttc caatgaacat gcccacgctg
      961 acaagagagg cccagttgcg tgcggttgca gccgcgactc cgtaggcggt ttgataccac
     1021 gagaccgccc agagtccctg aaaagagagg agcgctcccg ccgagacggt ccagagaacg
     1081 agcaacgacc tgaggtaggc ggagctcctg atgagccgga gcgcttcggc caggcgggag
     1141 agaatcgact gcgtcttttg ctccgtgaag ggcgtacggt gcacgacagg atcgaagctg
     1201 cgctccttcc agagaagcac gctcatggaa atgctcaaaa gtgtgagccc tgcgaaggtc
     1261 gccgagagac cgaatgcgtc cagagcgagt ccgagagggg cgacggaggc gagggcgccg
     1321 aatgcgccga cggagatggt gatgcttgag tagaagacgt acttgtccgg ggtgaacgcg
     1381 cttgcctgat aaacgagcgc gccgccgtac atgggggcca gcccgaaacc ggtaagcaga
     1441 cgccatgccc ccagcgtcca ttccgaaggg tggagcacaa ggagaaacgt acccgccgcg
     1501 gccaccagca tccccccggc gacgacgcgg agcggcccga aacggtcgtg cagggcgccg
     1561 gagatcgact gcataacgga gtaggcgtag tagtaaaggc tggaaataaa gccggtgaga
     1621 gcggcgctca tgccgagttt tgccgcttgc gagggaagga ccaccgatgc ggagatccgg
     1681 aagaaaaatg aaaagaaata cgccccgaca aggcagagaa ataaaaaatg agcgcggcgc
     1741 gaggatatgc cgcgggcttc gtcccgggat gatggtgagg cgtggcgcat aaaaatgagc
     1801 tccttccttt gccgctcctt tatcgccgct gagaagcgtc gtgtgaactt ggcggtcggc
     1861 aagcgtttca agcggttgtt ctgtatcacg gtgcatctta cctcataaac ggggaagagg
     1921 gtaggtatga ccgccgtttt taagaggggc tgaagaagcc ggagagtgcg tttcgaaaaa
     1981 tataagttga ctgtccttga atcgtagcgc ttcatgcttg acaggaattg aagcgcgaag
     2041 taagataagt ctaaccaaaa agtaagtctg ctctcacttc tcgaaaggag ggaatgtcgt
     2101 gtcggtctca tcccggattg aggactatat ggagacaatc ttcgccatag agatttccgg
     2161 acgggaagct acg
//
LOCUS       DAOPKU010000519         2171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_58908_length_2171_cov_5.298677, whole genome shotgun sequence.
ACCESSION   DAOPKU010000519 DAOPKU010000000
VERSION     DAOPKU010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58908_length_2171_cov_5.298677"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..930)
                     /locus_tag="PK849_13045"
     CDS             complement(70..930)
                     /locus_tag="PK849_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HPE67099.1"
                     /translation="MNTLHSARTFRGVVKIGGARGNTFLPLLEELAARVKRGEQWALV
                     HGASSMMEDLSRAVGTEPLYVTSPGGFRSRFVGEKELAIFEAACCRFSVDLVSALGRL
                     GVRAVPLYPSSAKGASAKRKDSLRSVENGKVRMLRGNYSGSIVSFDPSDVFAVWESGG
                     LPLLPPLAADEAVAGSNLNVDGDRMAAAAAAAVNADVLAILSNVPGLLRDPSDPSSKV
                     ESGTLSEWETIELFAQGNMKRKLLAAKEALEGGAETVLIADSRSAQPIEAGLSGGGTL
                     LCRGSMAAAG"
     gene            complement(961..2001)
                     /gene="argC"
                     /locus_tag="PK849_13050"
     CDS             complement(961..2001)
                     /gene="argC"
                     /locus_tag="PK849_13050"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPE67100.1"
                     /translation="MFRAVVWGASGMAGGEVLRILAAHPSIEAVCAVSRGKQGQPVWH
                     THPHLRASFPELAFSAPEETLKHEADVAFLALPHGTSQPLIKEYLERGVKVADLSGDV
                     RLKSAADYEKWYGAAPRHPELLERAVYGLPELHRTELQGASLASGVGCNASCSILGLY
                     PLARAGILGDVRIEVRVGSSEAGATPTLGSHHPYRSRTLRVYEPFRHRHLAEILQELD
                     LSEERVTMTMTAVEMIRGVQMIAQVSLSRPVKEAELWKLYRSAYADEPFVSLCPARPS
                     HLRLPDPKLVTGSNRVLTGFSLHEDGTRLVVVCALDNLMKGAAGSAVQSANLLLGLPE
                     TEGLNMLPVYPA"
BASE COUNT          372 a          738 c          696 g          365 t
ORIGIN      
        1 cgcagtagaa gtcgaggtac tcccgacctt cgctgtcctt gacggtcgcg cccttcccgg
       61 acgcgatgct tatcccgcgg ctgccataga tccccgacac agcagagtac ctcctcccga
      121 aagaccggct tctatcggtt gcgcgcttcg gctgtccgcg atgagcaccg tttccgcgcc
      181 cccttcgagg gcttctttcg cggccagcag cttccgcttc atattcccct gcgcgaagag
      241 ttcgatcgtc tcccactctg agagcgtgcc gctctccacc ttcgaggacg ggtcggaggg
      301 atcgcggagc agccccggaa cgttgctgag gatggcgagg acgtccgcat tcaccgccgc
      361 agccgcggcc gcggccatcc ggtcgccgtc cacgttgagg ttcgatcccg caacggcctc
      421 gtccgccgcc aggggaggaa ggaggggcaa accgcccgac tcccagacgg cgaagacgtc
      481 cgaagggtcg aacgacacga tggagccgct gtaattcccg cgaagcatcc tgaccttgcc
      541 gttctcgacg ctccggagag agtccttccg cttggcggac gctcccttcg ccgacgaggg
      601 gtagagaggg acggcgcgga cgccgagacg gccgagcgcg ctcacgaggt cgaccgagaa
      661 gcggcagcag gccgcctcga aaatggccag ttccttctcg ccgacgaaac ggctgcggaa
      721 gccgcccggg ctggtgacgt aaagaggttc cgttccgaca gcgcgggaga ggtcctccat
      781 catggagctt gcgccgtgga cgagcgccca ctgctctccg cgcttgacgc gcgccgccag
      841 ctcctccaga agaggaagaa atgtattccc gcgtgcgccg cctatcttga ccacgccccg
      901 gaatgtccgt gccgaatgca gagtgttcat ggcagaagct cctttctata gagtgatgcg
      961 ctacgccggg tagaccggga gcatattgag cccttcggtt tcgggcaggc cgagcagaag
     1021 gttcgccgac tggaccgcgc ttcccgcagc gcccttcatc agattgtcca gcgcgcagac
     1081 gacgacgagc cgcgtaccgt cctcatgcag cgaaaagccg gtgaggacgc ggttgcttcc
     1141 cgtgaccagc ttggggtccg gaaggcgcag gtgcgagggg cgcgccgggc agagcgagac
     1201 aaacggctcg tccgcgtagg cgcttctgta cagcttccac agctccgcct ctttcacagg
     1261 tcgggagagc gaaacctggg cgatcatctg gacgccgcgg atcatctcga ccgccgtcat
     1321 cgtcatggtg acgcgctcct cggagagatc cagctcctgg agaatctccg cgaggtggcg
     1381 gtgacggaac ggctcgtaga cgcggagcgt cctgctgcgg taggggtggt ggctgccgag
     1441 cgtcggcgtc gcgccggcct cggaggagcc caccctcacc tcgatgcgca cgtccccaag
     1501 gattcccgct cgggcgaggg ggtagaggcc gaggatggag caggaggcgt tgcaccccac
     1561 gccgctggcg agggaagctc cttgcagctc cgttcggtgc agctccggaa ggccatagac
     1621 ggcgcgctcc agaagctccg gatgacgggg ggccgcgccg taccacttct cgtagtccgc
     1681 tgcgctcttc aggcggacgt ctccggaaag gtcggccacc ttgaccccgc gctcgagata
     1741 ctcctttata aggggctggg aggttccgtg cgggagcgcc aggaaggcga cgtccgcctc
     1801 gtgcttcaga gtctcttccg gcgcgctgaa cgccagctcg gggaacgacg cccgcagatg
     1861 cgggtgcgta tgccagaccg gctgcccctg tttgcctctc gacaccgcgc agaccgcctc
     1921 gatagaggga tgcgccgcca aaatccggag gacctcgcct cccgccatgc cgcttgctcc
     1981 ccagacaacc gctcgaaaca tcgtcttcat cccttccgaa tcatttctgc gccgcgaggg
     2041 accagacgtg ctccacgagc cgccccgcga tatcgacgcc cgtgagttcg ccgtccgagc
     2101 tgcggaactc gggctgcccg ttcacttcat tcaccatcca gccaccttcc ggcgtctcga
     2161 aaaggtcgac a
//
LOCUS       DAOPKU010000520         2165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_59137_length_2165_cov_5.015640, whole genome shotgun sequence.
ACCESSION   DAOPKU010000520 DAOPKU010000000
VERSION     DAOPKU010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59137_length_2165_cov_5.015640"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1870
                     /locus_tag="PK849_13055"
     CDS             <1..1870
                     /locus_tag="PK849_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE67101.1"
                     /translation="TAFEPSVGRDVLLSMIEQANSKAAHEGAGRLDVFYRSQVTSVHR
                     RGPGIHGVTAVLDGRKTDIVCSVLIDATEYGDMLPLANALYRAGNSKSPVIDSSARIQ
                     DITWVAVIKKYPGGIPQNLRITTPPPGYVKYLDGFRQIVADGGNSFRKYPLRMPVDFP
                     THNAYRGLPDSSNPLDYTAAGADEWAKITKTGVNWGNDFPGAEQWEGKGGLPVAYLED
                     PAFRRDVNAQALLKTLNFIYYVQHELREPWSVADDEFYSEIPVTKTLGIVPDEYAEIV
                     RRFPPMPYVRESRRLVGIETPTSKTVRHNSESYRDEREGREIATSMAIGGYILDLHAG
                     DTDADLETEFGESFASIRTDMPQGPFQVPFGSFIPTEVDGLLVTEKNLSMSRLVSGAL
                     RLQPISMLTGQAAGTIAALSVREGKAARALDPRLVQLHLLEAGSALSLCEYHDVPRNH
                     LFWPGVQMSNLYGWFTPLDLPSSPSAKIDDIYNNRVVLARLFGLDKGVFGVDAPLTGA
                     EAETLLHKAFGEEKDASLYALPTFGLSSFISRGDFADALARILGYRATPEEFVSRFSD
                     ISSTSRLAGPMHFLSEKGVLDRVGASGVFMPDSLLTRGAAADMTMRAVVAPRERRR"
     gene            1867..>2165
                     /locus_tag="PK849_13060"
     CDS             1867..>2165
                     /locus_tag="PK849_13060"
                     /inference="COORDINATES: protein motif:HMM:NF020475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HPE67102.1"
                     /translation="MKCHLCETKSCSSGEACADFESLELYQGNEEDLQLLKTASDVEA
                     LYYCTLNRLEEIMEFARRMGYTRLGIAFCVGFKEEAALLGKILSEEFEVYSACCKV"
BASE COUNT          458 a          602 c          658 g          447 t
ORIGIN      
        1 gactgctttc gagccatcag tgggcaggga cgtcctgctg tcgatgatcg agcaggccaa
       61 cagcaaggct gcgcacgagg gggcggggag gctggatgtg ttctaccggt cgcaagtcac
      121 ctccgtccac aggcgcggcc cgggaatcca tggagtgacc gcagtcctcg acggccggaa
      181 aacggatatc gtgtgttcag tcttgatcga cgctaccgag tacggcgaca tgctcccgtt
      241 ggcgaacgct ctttacaggg cgggaaattc gaagagtccg gtcatcgact ccagcgccag
      301 aatccaggat atcacctggg tggcggtgat aaaaaagtat cccggcggaa ttccgcagaa
      361 tctcagaata acaacgccgc ctcccggata tgtgaagtac ctcgacggtt tccggcaaat
      421 tgtggcggac ggaggaaact ccttcaggaa gtatcctctc cggatgccgg tagacttccc
      481 tacgcacaat gcctacaggg ggctgcctga ttcgtcgaac cctctggatt acactgccgc
      541 cggtgcggac gaatgggcaa agatcaccaa gacaggggtt aactggggga acgacttccc
      601 cggcgctgaa cagtgggaag gaaagggcgg cctgcccgtg gcgtacctcg aagatccggc
      661 atttcgccgg gatgtgaacg cacaagcgct gttgaagacg ttgaacttca tttactacgt
      721 acagcacgaa ctgcgcgagc catggtccgt ggcggacgac gagttctact ccgaaattcc
      781 ggtaacgaaa actcttggca tcgtcccgga cgaatacgcc gagatagtca ggcgctttcc
      841 cccgatgccg tatgtgcgag agagccgccg tcttgtcggc attgaaaccc caacctcgaa
      901 aacggtccgg cacaactccg agagttatcg cgacgaacgc gaaggacgag agatagcgac
      961 gtccatggca atcggcgggt acatccttga cctgcatgca ggggataccg acgcggatct
     1021 cgaaacggaa ttcggcgaga gctttgcgtc gatccggacc gatatgccgc aggggccttt
     1081 tcaggtcccc ttcggcagtt ttatcccgac tgaagtggac ggcttgctcg ttacggaaaa
     1141 gaacctctcg atgtcccgtc ttgtgagcgg cgcgctgcgc cttcaaccaa tttcgatgct
     1201 cactggacag gctgcgggaa cgattgcggc gctctcagtc agagagggaa aagcggcgag
     1261 ggctctcgac ccgaggcttg tgcagctgca tctgctggag gcagggagcg cgctctccct
     1321 ctgcgaatac cacgacgtcc cgcgaaatca tctcttttgg ccgggcgtcc agatgtccaa
     1381 cctctacgga tggttcacgc cccttgacct gccttcgtcc ccttcggcga aaatagacga
     1441 tatctacaac aaccgggttg tgctggcccg tctcttcgga cttgataaag gggtgttcgg
     1501 cgtcgacgcc ccattgacgg gggcggaggc ggagacgctg ctccataaag cgttcggtga
     1561 agagaaggac gcttcgctgt atgcccttcc tacattcgga ctttcgtctt ttatctcgag
     1621 gggggatttt gccgacgcgc tcgcgaggat attagggtac agagcaactc ctgaagagtt
     1681 tgtctcccgc ttcagcgata tttcctccac ctcgcgtctc gcgggcccga tgcacttcct
     1741 ctcggagaag ggcgttctgg accgcgtcgg ggcatccggc gtctttatgc cggactcttt
     1801 gctgacgcgc ggagctgccg cagacatgac gatgagagca gttgtcgcgc cgagggaacg
     1861 acgaagatga agtgtcacct ctgcgagacg aagagctgct cctcgggtga ggcgtgcgct
     1921 gattttgaaa gcctggaact ctaccaggga aatgaagaag accttcaact tctcaagacg
     1981 gcctccgatg ttgaagcgct ctactactgt accctcaacc ggctggagga aataatggag
     2041 tttgcccggc ggatggggta tacgcgtctg ggcattgcct tctgtgtcgg ttttaaagag
     2101 gaggcggctc ttctggggaa gatcctttcg gaggaattcg aagtgtactc cgcatgctgc
     2161 aaggt
//
LOCUS       DAOPKU010000521         2155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_59554_length_2155_cov_3.045714, whole genome shotgun sequence.
ACCESSION   DAOPKU010000521 DAOPKU010000000
VERSION     DAOPKU010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59554_length_2155_cov_3.045714"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1463
                     /locus_tag="PK849_13065"
     CDS             276..1463
                     /locus_tag="PK849_13065"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HPE67103.1"
                     /translation="MTRYDFDTVIDRRNTDCAKWDGMKPLFGTNDLLPMWVADMDFKA
                     PPEVIEALRERVEHGIFGYTRRFEPYREAIAGWFLRRHGWEIRTEWIRHAPGVVPALA
                     AAIMAFTQPGEGILVQPPVYYPFFSTIKGRGRTVVENPLKLVNGRFEMDFDDLEKKLD
                     NSAVKLVFLCSPHNPVGRVWSREELEKFGAICAAKGVVVVSDEIHCDILYKGAKHTPL
                     ASVSDAMRESTITTVAPSKTFNIAGLATAAVVIPSKRLRDNYQSVIDFVNIDGGNVFG
                     ATALEAAYTKGDAWLDELLRYLEGNLDFIDTFLAEHLPEVSLLRPEGTYVPLLDCRSL
                     NLSGPELERLLVEKGKVALDGGHWFGTGGDGFARINIATPRALLKEGMEGIEKALKGY
                     RNR"
     gene            complement(1678..>2155)
                     /locus_tag="PK849_13070"
     CDS             complement(1678..>2155)
                     /locus_tag="PK849_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPE67104.1"
                     /translation="LGELVIPENEPGSSIMPGKVNPTQCEAVTMVAVQVMGNDAAVGF
                     AASQGNFELNVFMPVLIYNFLQSCRLLGDVMDSFERNAVRGMEADTQRIKKYLNESLM
                     LVTALTPRIGYDKAAEIAKKAHKEGSTLKEAAVALRYLTEDEFDSAVVPEKMVGPR"
BASE COUNT          488 a          594 c          571 g          502 t
ORIGIN      
        1 attggaagaa catggcgcct aagccctctc tttgtagaag cagttttgca cacattttgt
       61 tatgaacttt ccatcaaggt tatgatttcg ttccaaataa agacgccgtg agactatttc
      121 cctgggcgta aatcgtaaag ctttgcattt ttagtataac tctggaattg ttcgcttttg
      181 ggggacggaa aagcagtttt tctcaatgca gcatcttctc ctcgtttaaa aaaatgatat
      241 gatagtagca acttgttgaa agcgaggttc gcattatgac gcgctacgac ttcgatacgg
      301 tgatcgacag aagaaatact gactgcgcca agtgggacgg catgaaaccg ctcttcggga
      361 caaacgatct gcttccgatg tgggtggcgg atatggattt taaagcgccc cccgaggtaa
      421 tcgaagccct ccgggagcgt gtagaacacg gtattttcgg ctacacccgc cgttttgaac
      481 cgtatcgcga ggcgatcgca ggatggttcc ttcgcaggca cggctgggaa atacgcaccg
      541 agtggattcg tcacgccccc ggagtcgttc cggcgctcgc ggccgccata atggccttta
      601 cacaacccgg ggaaggaata ctggtgcagc ctcccgtcta ctaccccttc ttctccacca
      661 tcaagggaag ggggcgcacc gtcgtagaga accccctgaa gctggtcaac ggccgttttg
      721 agatggactt cgacgacctt gaaaagaagc ttgacaactc cgcggtcaag ctcgtcttcc
      781 tctgctctcc ccacaacccg gtaggacgcg tctggagcag agaagaactc gaaaagttcg
      841 gcgcgatctg cgccgcgaag ggcgttgtag tcgtctccga cgagattcac tgcgacatcc
      901 tttataaagg agcgaagcat actccgctcg cttccgtctc cgacgctatg agggagagta
      961 ccatcaccac cgtcgcgcca agcaagacat tcaacattgc gggacttgcc acagcggcgg
     1021 ttgtcatccc ttcgaaacga ctgcgcgaca actaccagtc cgtcatcgat ttcgtcaaca
     1081 tcgacggcgg caacgtcttc ggcgccacag cgctggaagc ggcatacacc aagggagacg
     1141 catggcttga cgagctgttg cgctatcttg aaggcaatct ggacttcatc gacacatttc
     1201 tcgcagagca tctgccggaa gtttcgctcc ttcgccccga ggggacctat gtaccgctgc
     1261 tcgactgccg ctcgctgaat ctctccggac cggagctgga aaggctgctc gtggaaaagg
     1321 gcaaagtggc tctcgacggc ggacactggt tcggaacggg cggcgacggg tttgccagaa
     1381 tcaacatcgc cacgcccaga gcgctgttaa aagaaggcat ggaagggatt gaaaaagccc
     1441 tgaaaggcta ccggaacaga tagcggagcg tatcgacgcc ctccatcttt tgtaagctca
     1501 ttgtacaacg gtcggtgaag ggatgagcct tcgccggccg ttgtgcattt tctagatggg
     1561 ctggaggacg gctttcccga ctttgtcggt tgtagagagg gaagtattgc aatcaccgga
     1621 ttttgttttt tctccagtta ctacaagctc ctctttcttt ctaaaagaga ggacgttcta
     1681 tctcggtccg accatctttt ccggaacaac ggcgctgtcg aattcatcct ccgtcagata
     1741 gcgaagagcg acggccgcct ccttgagcgt gcttccctcc ttgtgcgcct tcttcgctat
     1801 ctcggcggct ttgtcgtatc ctatccgcgg cgtcagagcc gttacaagca tcaatgattc
     1861 gttcaaatac ttcttgatgc gttgcgtgtc cgcttccatg cctctgacgg cgttgcgctc
     1921 gaaagagtcc ataacgtcgc caagcagacg gcacgactgc aaaaagttgt agatgagtac
     1981 gggcataaaa acattcagtt caaagttccc ctgactggcg gcaaagccga cggccgcgtc
     2041 gttgcccatc acctggacgg ccaccatcgt aacggcctcg cactgcgtcg ggtttacttt
     2101 tccaggcata atcgaactcc ccggttcatt ctcaggaatg acgagttctc cgaga
//
LOCUS       DAOPKU010000522         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_59621_length_2153_cov_4.516206, whole genome shotgun sequence.
ACCESSION   DAOPKU010000522 DAOPKU010000000
VERSION     DAOPKU010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59621_length_2153_cov_4.516206"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PK849_13075"
     CDS             complement(<1..910)
                     /locus_tag="PK849_13075"
                     /inference="COORDINATES: protein motif:HMM:NF017180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family
                     metallo-endopeptidase-related protein"
                     /protein_id="HPE67105.1"
                     /translation="MMKLNCKPSSEPQRSRRAVHGFLILALLLAAFSAFSWPISANER
                     DRVPPLATEDDWQLIEFEGQLWAIENNPSGKYRLKNDLDLGGYENWRPIGRGQTTLTP
                     FRGQFDGAGFTIRNLTITGGDLDNAGFFAQIDPTGLVENLAFENANIEGRENVGILAG
                     VCKGTVTNVAAAGSVNGAQYAGGLVGLLDGGTVHDSSSAADVGGGDRLGGLVGFSQNG
                     EILRSRASGEISISGDFDHPGLAGIGGLVGQATKSVRISECFATGNVSGNYGAGAGGL
                     IGSVSSEGFNANISDCFAVGGVRGSKI"
     gene            complement(1281..1598)
                     /locus_tag="PK849_13080"
     CDS             complement(1281..1598)
                     /locus_tag="PK849_13080"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPE67106.1"
                     /translation="MNERYGLPEETIAMIAAVFTRHPEVERVILYGSRAKGNYRSGSD
                     IDLALQGGDGLTERVLYSIVRELDDLPVPYSFDVAIHSMIANEALLEHIRRRGTVVYE
                     RRR"
     gene            complement(1595..2014)
                     /locus_tag="PK849_13085"
     CDS             complement(1595..2014)
                     /locus_tag="PK849_13085"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HPE67107.1"
                     /translation="MEDIRWIQRFDSFLRALSQLEEAYALAASRRLSRLEEQGLIHTF
                     EFTHELAWKTLKDFLESRGTQDLYGSKDTTREAFRNGLVNDGDVWMDMIVSRNLTSHT
                     YDEATASRISARILENYFGEFKRFRAAMERFKETAPS"
BASE COUNT          399 a          595 c          639 g          520 t
ORIGIN      
        1 cgatttttga tcctctgacg ccgccgacgg cgaagcagtc cgagatgttc gcgttgaacc
       61 cctccgaaga caccgagccg atcaagccgc ccgcgccggc gccgtagttg ccggagacat
      121 tcccggtggc gaagcattcg ctgatccgca cgctctttgt cgcctggcct acgaggcctc
      181 cgattcccgc caatccgggg tggtcgaaat cgcctgaaat ggaaatttcg ccgcttgccc
      241 tggagcgcag gatttctccg ttctgactga aaccgacgag gccgcccaga cggtctccgc
      301 cgccgacgtc ggccgcgctg gaagagtcgt gtactgttcc tccgtcgagg agtccgacga
      361 gtccgcccgc gtattgggct ccgttgacgc tgcccgccgc agcgacgttt gtgaccgttc
      421 ccttgcagac gcccgccagg atgccgacgt tttcccttcc ttcgatgttg gcgttttcga
      481 aggcgaggtt ttccaccagc cctgtgggat cgatctgtgc gaagaatccg gcgttgtcga
      541 ggtcgccgcc ggttatcgtc agattccgga tggtgaagcc tgcgccgtcg aactggcccc
      601 tgaacggggt aagcgttgtc tgtccgcgtc cgatcggtct ccagttttcg tagccgccga
      661 ggtcgaggtc gtttttcagg cggtatttcc ccgaggggtt gttctcgatg gcccagagct
      721 gcccttcgaa ctcgatcagc tgccagtcgt cctccgttgc aagaggaggt actcggtccc
      781 gctcgtttgc ggagatcggc catgagaatg cggaaaacgc cgcaagaagg agcgcaagga
      841 tcaagaatcc gtgtacagct ctccgcgagc gttgtggttc tgaagaaggt ttgcaattca
      901 gcttcatcat cgttgtcacc tccggttttt ttcagggtgc agaagatcgg tttttcgcaa
      961 gtccagcctg gtacgaatgc atcgtattca acaattgaaa gagctgctct gatgaagcgg
     1021 tggagaactt cttcaactgt ttgtattatt atattcatat tttcgctttg tcaagacgct
     1081 gattccgtcc gctgttcgca ggaaaccggt acagacgttt ttacgagaag aacgccggaa
     1141 aattacactg ctcgaagacc atagagctcc agggagttcc gcttcaggtt gccgtctacg
     1201 ggtgggagtt gcaaccgtag gcttattgat ggcatttttt ttctcggcga tgaggatgtg
     1261 ggtgaggaaa acgtttgccg ttaccggcgc ctttcataca ccaccgttcc tctgcggcgg
     1321 atatgctcaa gcagggcctc gttggcgatc attgagtgga tcgcgacgtc gaaggagtag
     1381 gggaccggaa ggtcgtcgag ttcgcgcacg atgctgtaga ggacgcgctc ggtcaggccg
     1441 tcgcctccct ggagcgcaag gtcgatatcc gaaccgcttc tatagttgcc tttggctcgc
     1501 gatccgtaga gtatgactcg ctcgacttcc ggatggcggg tgaagacggc ggcgatcatc
     1561 gctatggttt cttccgggag gccgtaccgt tcgttcatga cggggccgtt tctttgaagc
     1621 gttccatcgc ggcgcggaag cgtttgaatt cgccgaagta gttctccagg attctggcgg
     1681 agattctgga ggccgtggct tcgtcgtagg tgtgcgaagt aaggttgcgg ctgacgatca
     1741 tgtccatcca gacgtcgccg tcgttgacga ggccgttccg gaaggcttct cgcgtggtgt
     1801 ccttcgagcc gtagaggtcc tgggttcccc tgctttcaag gaagtccttc agcgtcttcc
     1861 atgccagttc atgggtgaat tcaaacgtgt gaatgagccc ctgctcctcg aggcgggaga
     1921 gccgacgcga cgcggcaagc gcgtatgctt cttcaagttg ggaaagcgcg cgaagaaagc
     1981 tgtcgaatcg ctgtatccac cggatatctt ccatcggtga agcccctcct ttttttaaac
     2041 aactcccaac gctcataata gcatacagcc atcggtcttt cttgaacagg agtccggaaa
     2101 gaaggccgag ccggtttcct gagaggcatc atgctctaat ggacgctctc tct
//
LOCUS       DAOPKU010000523         2150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_59721_length_2150_cov_5.461098, whole genome shotgun sequence.
ACCESSION   DAOPKU010000523 DAOPKU010000000
VERSION     DAOPKU010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59721_length_2150_cov_5.461098"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK849_13090"
     CDS             complement(<1..406)
                     /locus_tag="PK849_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67108.1"
                     /translation="MISRGVRAALCAWFTLAFAAGAFAAPATVVFSKDGGVFLTDSEG
                     KQPVKLAEGSNPEVSPDGRFVAFTASSGKDGWGRRIGVVEAASKKATVLKTIPGENSY
                     GPRWSPDGKSLLFNHWDEGSSDWVFGVATIADG"
     gene            complement(582..1010)
                     /locus_tag="PK849_13095"
     CDS             complement(582..1010)
                     /locus_tag="PK849_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPE67109.1"
                     /translation="MNRLTPFLFFAAGLSADVLSKEWALRTLPPPTLHAGGDFFALAL
                     HFNRGAAFSLLRSVPGAALLLSVAGVCLLAAMYRSLPAFRRSPAWGLLFAGAAGNMAD
                     RLLHGYVVDWFRAGPLHMNAADILLCAGGALLMLQMLRKK"
     gene            complement(1042..>2150)
                     /locus_tag="PK849_13100"
     CDS             complement(1042..>2150)
                     /locus_tag="PK849_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPE67110.1"
                     /translation="REALWERTLEFSRAFARNQDVDAAISSTLDTLRRDVKAFGVLLS
                     SVEGNSFRLTAWSGYNGELADFVVSKDGIAASESIISRSPVWVEDVRTHPTAHPVHPS
                     VKSELLIPLFQAGEEEGVLEVAFDRPMRRDSFLIETLTPVASFLGGLVHGEKMRRELK
                     ASYAYLAEKLQFVTGIYHDETEAHIARIGEYCKLLAREIGRDASEQEDIALFARLHDI
                     GKLRIPYGILVKPGTLDTDEFEQIKRHPQWGAEILGDAAWLQMARNICLTHHEKWDGS
                     GYPRGLHGTEIPWEGRVATIADIYDALRSSRSYKPAMSHELAVSILLEGDERVRPEHF
                     DPKVLTAFRRISGKFEEIFDSNCDERDSPDCFLA"
BASE COUNT          374 a          706 c          684 g          386 t
ORIGIN      
        1 tcccgtccgc tatcgtcgct accccgaaca cccagtcgga actcccctcg tcccagtggt
       61 tgaagaggag cgacttcccg tccggcgacc agcgcgggcc gtagctgttc tcgccgggga
      121 tcgtcttcag gacggttgct ttcttcgacg ccgcctcgac aacgccgatt ctccgtcccc
      181 acccgtcctt tcctgaggaa gccgtgaagg cgacgaaccg cccgtccggc gagacctccg
      241 ggttgcttcc ctcggccagc ttcaccggtt gcttcccctc cgaatcggtc aggaaaacgc
      301 ccccgtcctt tgaaaaaacc accgtcgccg gagcggcgaa cgcccccgcc gcgaacgcga
      361 gcgtaaacca tgcgcacaac gccgcgcgca ctcccctgga aatcatcgct accgcctcct
      421 tcatgcatga ttgaacggac gtccgctgtg gacagccgtt accctcggat gcaggttatg
      481 cacttgttgt ggatacgttg tccacaataa agcgtctgtt ttcccctttt ctatgatcgt
      541 atcgcccgga aacactgcgc agcgtgcggc gtttttagaa gttacttttt ccgcagcatt
      601 tggagcataa gcaacgcgcc gccggcgcag aggaggatgt cggcggcgtt catgtggagg
      661 gggcctgcgc ggaaccagtc gaccacgtag ccgtggagca gcctgtccgc catgtttccc
      721 gccgcgcccg caaagaggag cccccacgcg ggcgagcgcc ggaaggccgg gagcgacctg
      781 tacatcgcgg caagcaggca gacgcccgcc acgctcaaca gcagcgcagc gccggggacg
      841 gagcggagaa gggagaatgc ggcgccccgg ttgaagtgga gggcgagcgc gaaaaaatcc
      901 cctcctgcat ggagcgtcgg aggggggagc gtccgaaggg cccactcctt ggagagcacg
      961 tccgcggaaa gtcccgcggc gaaaaaaaga aacggcgtca ggcggttcat gatgtgcgag
     1021 gcgcccgggt catcttctcc gctacgcgag gaagcagtcc ggcgagtcgc gctcgtcgca
     1081 gttggagtcg aagatctcct cgaacttccc ggagattcgc ctgaacgcgg tgagcacctt
     1141 cgggtcgaag tgctccgggc gcaccctctc gtccccctca aggaggatac tgaccgccag
     1201 ttcatgcgac atcgcgggct tgtacgagcg ggacgaccgg agcgcgtcgt agatgtccgc
     1261 gatggtggcc acccgtccct cccatggaat ctccgtaccg tgcagtccgc gggggtagcc
     1321 gctgccgtcc catttttcgt ggtgcgtcag gcagatgttc ctcgccatct gcagccacgc
     1381 cgcgtcgccg aggatttcgg ctccccactg aggatggcgc ttgatctgtt cgaactcgtc
     1441 ggtgtcgagc gttcccggtt tcaccaggat gccgtacgga atccgcagct tgccgatgtc
     1501 gtgcagccgg gcgaagaggg cgatgtcctc ctgctcgctt gcgtccctgc cgatctcgcg
     1561 cgcgagcagc ttgcagtact cgccgatgcg ggcgatatgc gcctccgtct cgtcgtggta
     1621 aatccccgtg acgaactgga gcttttccgc gaggtaggcg taggaggcct tcagctcgcg
     1681 gcgcatcttc tctccgtgga ccagtccgcc gaggaaggag gccaccgggg tgagcgtttc
     1741 gatgaggaag gagtccctcc gcataggccg gtcgaaggcg acttcgagta cgccctcctc
     1801 ctcgccggcc tggaagagcg gaatcagcag ctcgcttttt accgaagggt gcaccggatg
     1861 ggccgtgggg tgcgtacgga cgtcctccac ccagacgggc gagcgggaga tgatgctctc
     1921 cgaggcggcg atgccgtcct tcgagacgac gaagtccgcg agctctccgt tgtagccgct
     1981 ccatgcggtc agccggaagc tgttcccctc aaccgaggag aggagcaccc cgaacgcctt
     2041 cacgtccctt cgcagcgtat cgagcgtgga ggagatcgcc gcgtccacgt cctggttgcg
     2101 ggcgaaggcg cgggagaact ccagagtgcg ctcccacagg gcttcccggc
//
LOCUS       DAOPKU010000524         2149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_59766_length_2149_cov_3.753582, whole genome shotgun sequence.
ACCESSION   DAOPKU010000524 DAOPKU010000000
VERSION     DAOPKU010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59766_length_2149_cov_3.753582"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PK849_13105"
     CDS             <1..1021
                     /locus_tag="PK849_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE67111.1"
                     /translation="AGSLAQISVIMSTLMGGVSGSANADAVMECRILGPEMIKQGYSR
                     GYGAAVNGLTAMITCTIPPSMGFIIYGSVGEVSIGRLFVGGIVPGFLMMAMMMVTVNF
                     TAKRRGYLPTTDKKPTAGEVLSAVKENIYALIFPVILIAGIRFGLFTPSEAGAFAAGY
                     ALFVGVFIYKEITWKVFIDTVNQSAVDIGVLMLILTLSGTFGYAIVYGRVPQSIAEFL
                     IGITSNQYLLMSLIIVMLILAGMFVETTVCALLLTPVFIPVITRLGIDPVHFGVIMMT
                     TLTSGIMTPPVGIALYSTSEIMGCTPQETVKEAIPFYITLFALIVVLVLFPQIVLFLP
                     NRVFG"
     gene            1091..>2149
                     /locus_tag="PK849_13110"
     CDS             1091..>2149
                     /locus_tag="PK849_13110"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HPE67112.1"
                     /translation="MKLAMVFYRHHLTKENFRYAKQLGCTHLVIHLVDYFNREKDPVT
                     GDNQPVGDSDGWGVTRNREVWSYDELRAIRNMVDEEGLVLEALENFDPGFWYDVLLDG
                     PKKIAQMDGLKQLIRNVGQAGIPIMGYNFSLAGVAARISGPFARGGAESVGCDGCDTL
                     PLPEGMVWNMVYDPEAPKGVNAATQITSEELWSRFEWFLKELLPVAEESNVKMAAHPD
                     DPPFAVVRGTPRLVWRPELYRRLLDLYPSHCNTLELCVGTLAEMNGVNYYRAIDEFAA
                     RDAIGYVHLRNVRGQVPNYRETFIDDGDIDIYRVLKILHANDFRGAIIPDHSPLVSGP
                     APWHTGMAYALGYIRAMMQ"
BASE COUNT          392 a          700 c          672 g          385 t
ORIGIN      
        1 ggcgggcagc ctggcgcaga tcagcgtcat catgagcacg ctgatgggcg gcgtctccgg
       61 ctcggcgaac gccgacgcgg tgatggagtg ccgcatcctg ggccccgaga tgatcaagca
      121 ggggtactcg cgcggctacg gtgcggcggt caacggtctc accgcgatga tcacctgcac
      181 catccctccg agcatgggct tcatcatcta cggctcggtg ggggaggtct ccatcggcag
      241 gctcttcgtc ggcggcatcg tcccgggatt cctgatgatg gccatgatga tggtcaccgt
      301 caacttcacg gcgaagcgcc gcggctatct gcccaccacc gacaaaaaac cgaccgcggg
      361 cgaggtcctg tcggcggtga aggagaacat ctacgcgctg atcttcccgg tcatcctgat
      421 cgcaggaatc cgcttcggcc tgttcacgcc ctccgaggcg ggagcgttcg ccgcggggta
      481 cgcgctcttc gtcggcgtct tcatctacaa ggagatcaca tggaaggtct tcatcgacac
      541 ggtgaaccag agcgcggtgg acatcggcgt gctcatgctc atcctcacgc tctccggcac
      601 gttcggctac gcgatcgtct acgggcgcgt gccgcagtcg atcgccgagt tcctgatcgg
      661 gatcacctcc aaccagtacc tgctgatgtc cctgatcatc gtcatgctga ttctggcggg
      721 gatgttcgtc gagacgaccg tgtgcgcgct gctgctgacc cctgtcttca tcccggtgat
      781 cacgcggctg ggcatcgacc cggtgcactt cggcgtcatt atgatgacga cgctgaccag
      841 cgggatcatg acgccgccgg tgggcatcgc cctctactcg acgtcggaga tcatggggtg
      901 cacgccccag gagacggtga aagaggcaat ccccttctac atcacgctct tcgccctcat
      961 cgtcgtcctg gtgctctttc cgcagatcgt cctcttcctg cccaaccggg ttttcgggta
     1021 atatacgcgg gaggctttca ataacgacgt tgccacaggc tcttcagagc cgcgaaggga
     1081 gaagtgacgc atgaaactcg caatggtctt ctaccgtcat cacctgacga aggagaactt
     1141 ccgttacgca aagcagctcg gctgcaccca cctggtgatc catctggtgg actacttcaa
     1201 cagagaaaag gaccccgtca cgggagacaa tcagcccgtc ggggactccg acggctgggg
     1261 cgtgacccgc aaccgcgaag tatggagcta cgacgagctg cgcgccatcc ggaacatggt
     1321 ggacgaggag gggctcgtac tggaggctct cgagaacttt gaccccggct tctggtacga
     1381 cgtcctgctc gacggcccga agaagatcgc gcagatggac ggcctcaaac agcttatccg
     1441 caacgtcgga caggcgggca tcccgatcat ggggtacaac ttcagcctcg caggggtggc
     1501 ggcgcgcatc agcggcccgt tcgcccgcgg gggggcggag tcggtcggct gcgacgggtg
     1561 cgatacgctg ccgctgccgg agggaatggt gtggaacatg gtctacgacc cggaggctcc
     1621 gaagggtgtc aacgcggcga cgcagatcac ctcggaagag ctgtggagcc gcttcgagtg
     1681 gttcctgaag gagctgcttc cggtggcgga ggagtccaac gtgaagatgg cggcgcaccc
     1741 cgacgatccc cccttcgccg tcgtccgggg aacgccgcgc ctcgtctgga ggcccgaact
     1801 ctaccgccgc ctcctggacc tgtacccgag ccactgcaac acgctggagc tgtgcgtcgg
     1861 cacgctcgcc gagatgaacg gcgtcaacta ctaccgcgcc atcgacgagt tcgccgcgcg
     1921 ggacgccatc ggctacgtcc acctgcgcaa cgtccgcgga caggtgccca actacaggga
     1981 gactttcatc gacgacggcg acatcgacat ctaccgggtg ctcaagatac tgcacgccaa
     2041 cgacttccgg ggcgcgatca tccccgacca ctccccgctg gtcagcggcc ccgctccgtg
     2101 gcacacgggc atggcctacg ccctcggcta catccgcgcc atgatgcag
//
LOCUS       DAOPKU010000525         2137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_60202_length_2137_cov_4.140730, whole genome shotgun sequence.
ACCESSION   DAOPKU010000525 DAOPKU010000000
VERSION     DAOPKU010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60202_length_2137_cov_4.140730"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PK849_13115"
     CDS             <1..803
                     /locus_tag="PK849_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE67113.1"
                     /translation="SGAQSSAQKGTEMANEVEQARLAGEEGTKAVEKAVASINGVAKE
                     SERSAQQVKSLGDRAREIQSFVTQIGGIADQTNLLALNAAIEAARAGEAGRGFAVVAE
                     EVRKLAEESNEAAKQIAALAAGITKDLDSVVTSSEQGAKASKDSSVLAEETRTTIGRM
                     MEALSRISMATQDLAAVSEEQAASSEEIASAIQNIASRVSAAASSSDMVRGQMAEVGA
                     SAERVAQGSEELAGLAEDLKKLVGGFKFEDATHKGLVPVGNAPKGKKR"
     gene            904..1368
                     /locus_tag="PK849_13120"
     CDS             904..1368
                     /locus_tag="PK849_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPE67114.1"
                     /translation="MAEQLLVVFALGKEEFGIDISRVREIVRMQNITTIPQSMDFVEG
                     IVNLRGQIVPIVDLCKRFLVVDREGRNDIDRRIIVVHMAGQNIGILVDGVSEILRVPD
                     EAIEPTPPMVASGASVDFIRGVAKVKDRLIMALDLDRIFSTEERELLAQTES"
     gene            1446..1901
                     /locus_tag="PK849_13125"
     CDS             1446..1901
                     /locus_tag="PK849_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE67115.1"
                     /translation="MSRIEKTPTIMIVDDTPAILDLLDQMLRAQGYRALTFPRGKLAL
                     EAAEETPPDLILLDITMPEMDGFEICRRLKNTPRLAEIPVIFIGPLAEPDEKALAFAA
                     GGADYVTKPFQEEEILVKVRTYLRMMEMQRELRKREKTTGTPSGEKAPL"
BASE COUNT          494 a          606 c          709 g          328 t
ORIGIN      
        1 cgagcggcgc ccagtcctcc gcgcagaagg ggaccgagat ggcgaacgag gtggaacagg
       61 cgcggctcgc cggagaagag ggaacgaaag ctgtcgagaa agccgtcgcc agcatcaacg
      121 gcgtggcgaa ggagtcagaa cgttccgcgc agcaggtgaa gagtttgggc gaccgggcgc
      181 gggagatcca gagcttcgtc acccagatcg gcggcatcgc ggaccagacg aacctgctgg
      241 cgttgaacgc cgccatcgaa gcggcgcggg ccggagaggc cggacgcggc ttcgcggtcg
      301 tcgccgaaga agtccgcaag ctggccgagg agagcaacga ggcggcgaag cagatcgcgg
      361 cgcttgccgc agggatcacg aaggacctgg acagcgtcgt cacatcgtcg gaacagggag
      421 cgaaggcgtc gaaggactcc agcgtcctcg ccgaggaaac gcgcacgacg atcggcagaa
      481 tgatggaggc actctcccgg atatccatgg cgacgcagga tcttgccgcg gtctcggaag
      541 agcaggcggc gtcgagcgag gaaattgcga gcgctataca aaacatagcg tcccgcgtga
      601 gcgccgcggc ctcctcttcg gatatggtgc gcggtcagat ggcggaggtc ggcgcctcag
      661 cggaacgcgt agcgcagggt tccgaggagc tcgccggact tgccgaggat ctgaaaaagc
      721 tggtcggcgg cttcaagttc gaggacgcta cgcacaaagg actggttccg gtagggaacg
      781 cccccaaagg gaaaaaacgc tgacccctcc ggggagttac gcacacactc cctcgccgct
      841 cccggagcaa ctgacgcgcc cgggagcggc ttgaaaggac cgcatcgaaa aggaggaaga
      901 gctatggcag aacagctgct tgtcgtcttc gcactgggca aagaagagtt cggcatcgac
      961 atctccagag tgcgcgagat cgtgagaatg cagaatatca ccacaatacc gcagtctatg
     1021 gactttgtgg aggggatcgt caacctccgg gggcagatcg ttcccatcgt cgacctgtgc
     1081 aagcgttttt tggtcgtcga cagggaggga agaaacgata tcgaccggcg tatcatcgtc
     1141 gtccacatgg cggggcagaa catcggcatc ctggtggacg gcgtgtcgga gatactgcgc
     1201 gttccggacg aggccatcga gccgacgccc ccaatggtgg cgagcggcgc aagcgtggac
     1261 ttcatccgcg gagtggcgaa ggtgaaggat cgtttgatca tggcgctgga tctcgaccgt
     1321 atcttctcaa cggaagagag ggagctgctc gcccagaccg agagctgaga gaacgcgggg
     1381 gaggagtctc cccgctcccc cgcgttgctc cgccgcctgg caggcgggag gaggtacaga
     1441 gggaaatgag ccgcatcgaa aagacgccga ccatcatgat cgtggacgat actcccgcta
     1501 ttctcgacct gctggatcag atgctgcggg cgcaggggta ccgcgccctg acctttccgc
     1561 gaggaaaact ggcgctcgaa gccgcggagg aaacccctcc ggatctgatc ctgctcgata
     1621 tcacgatgcc cgaaatggac ggattcgaga tctgcaggcg attgaaaaac acgcctcgtc
     1681 tcgcggagat tccggtgatc ttcatcgggc cgctcgccga acccgacgaa aaggcactgg
     1741 ccttcgccgc aggcggcgcg gactacgtaa cgaagccttt tcaggaggag gaaattctgg
     1801 taaaggtacg gacatatctt cggatgatgg agatgcagcg cgaacttcga aagcgggaga
     1861 agacaacggg aacgccctcc ggagaaaaag cgcccctgta agctccgcaa ttcgaagaaa
     1921 aatagaatgt gcacgccgct gcgctgatac aatagaatca tgaaaaaaga aacggcgagc
     1981 cgtacgcttt ccggcgcgcc gtttccgccg aagcaggcca acgcggcacg gaggtgtatt
     2041 catgggttcg atgctcgaac agcacatact gcgcacccct ctgatcgggt actcatttcg
     2101 ccggatcatc ctcgacaact cggacagacc ggtcgac
//
LOCUS       DAOPKU010000526         2135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_60278_length_2135_cov_3.986538, whole genome shotgun sequence.
ACCESSION   DAOPKU010000526 DAOPKU010000000
VERSION     DAOPKU010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60278_length_2135_cov_3.986538"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /gene="fdhF"
                     /locus_tag="PK849_13130"
     CDS             <1..1686
                     /gene="fdhF"
                     /locus_tag="PK849_13130"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPE67116.1"
                     /translation="SVGGEEAATAVIDHCARLCHSSSVAGLAETLGSGAMTNDYESIR
                     HADVVLIIGSNTTETHPVIGSFIKERLKRGGCTLIVCDPRKIEMAKYADIDIRQKSGS
                     DVALLNGMMHVILRDGLHDAAFIEKHVNNFEKLKETVAKYTPEYVAKIAGVAPELIEK
                     AARAYAKGPNSAIFYTMGITQHVTGTNNVRSVANLALLCGMFGRPGTGVNPLRGQNNV
                     QGACDMGCLPNVVSGYQKTNDPATRAKIMKAWGRDLPETPGRTMTELMDAAEEGGLKA
                     AYIMGENGMVSEPDTEHVRHALKNLDFLVVQDIFLTETAELADVVLPAACWGEKDGTY
                     TSTCRAVQRIRKAVDAPGEARPDWEILLDLAKRLGAKWEMNSPEAIFDDLARITPQYA
                     GMSYGRLENAPLCWPCPTSEHPGTPVLYTAGFPRGKADFSPCEWLPPHEWPDEEYPFL
                     ATTGRLLYHYHTGSMTRRSAPGEFVKEMFVEVAPADARALECEDGDTVRVSSRRGSIS
                     AAVKVTDRVPTGMVFLPFHFAENAANTLTSNSLDPVCKIPGFKVNAVKVEKAV"
     gene            1832..>2135
                     /locus_tag="PK849_13135"
     CDS             1832..>2135
                     /locus_tag="PK849_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE67117.1"
                     /translation="MLQRVFRFTAALALVGALSLPLGAAELPRASLGYIFTTHQEPIM
                     VAMAKGEAFEPLGAYLKPVVDKERYDLFVDGAPVARISIVVTKSGSEMATLFAQNHL"
BASE COUNT          411 a          681 c          730 g          313 t
ORIGIN      
        1 tcggtgggcg gcgaggaggc ggccacggcc gtcatcgacc actgcgcccg cctctgccac
       61 agctcgagcg tcgcggggct ggcggagacg ctcggcagcg gcgcgatgac caacgactac
      121 gagtcgatac ggcacgccga cgtcgttctg atcatcgggt cgaacaccac cgagacgcac
      181 cccgtcatcg gctcgttcat caaggagcgc ctcaagaggg gcggctgcac gctgatcgtc
      241 tgcgatccgc gcaagatcga gatggcgaag tacgccgaca tcgacatccg gcagaagagc
      301 ggctcggacg tcgcgctgct gaacggcatg atgcacgtca tccttcgcga cggactgcac
      361 gacgcggcgt tcatcgagaa gcacgtcaac aacttcgaaa agctgaagga gaccgtggcg
      421 aagtacacgc ccgagtacgt ggcgaagatc gcgggcgtcg ccccggagct gatcgagaag
      481 gcggcccgcg cctacgcgaa ggggccgaac tccgccatct tctacacgat ggggatcaca
      541 cagcacgtta ccgggacgaa caacgtccgc tccgtcgcga acctcgcgct gctctgcggc
      601 atgttcggcc gtcccggaac gggcgtgaac ccgctgcgcg gccagaacaa cgtgcagggc
      661 gcctgcgaca tggggtgcct gcccaacgtc gtctccgggt accagaagac gaacgacccc
      721 gcgacgcgcg cgaagatcat gaaggcgtgg gggcgcgacc ttccggagac gcccggacgc
      781 accatgacgg agctgatgga cgccgccgag gaaggaggcc tgaaggccgc ctacatcatg
      841 ggcgagaacg gcatggtgag cgagcccgac acggagcacg tgcggcacgc cctgaagaat
      901 ctcgacttcc tcgtggtgca ggacatcttc ctgaccgaga cggcggagct ggccgacgtg
      961 gtgctccccg ccgcctgctg gggagagaaa gacgggacgt acaccagcac ctgccgggcc
     1021 gtgcagcgca tccgcaaggc ggtcgacgct ccgggcgagg cgcgccccga ctgggaaatc
     1081 ctgctcgacc tggccaagcg cctcggcgcg aagtgggaga tgaactcccc ggaggcgatc
     1141 ttcgacgacc tcgcccgcat caccccccag tacgcgggga tgagctacgg acgtctggag
     1201 aacgcgccgc tctgctggcc ctgcccgacg agcgaacacc ccggaacgcc ggttctgtac
     1261 accgccggtt tcccgagggg aaaggccgac ttctcgccgt gcgagtggct gcctccgcac
     1321 gagtggccgg acgaggagta ccccttcctc gccacgacgg ggcgtctgct ctaccactac
     1381 cacaccggga gcatgacgcg gcggagcgcc cccggcgagt tcgtcaagga gatgttcgtc
     1441 gaggtcgccc cggcggacgc ccgcgcgctc gagtgcgaag acggcgatac ggtgagagtg
     1501 tcgtcgcgca ggggctccat cagcgcggcg gtgaaggtga ccgaccgcgt cccgacgggg
     1561 atggtcttcc tcccgttcca cttcgcggag aacgccgcga acacgctcac ctcgaactcc
     1621 ctcgacccgg tctgcaagat accgggcttc aaggtgaacg cggtgaaggt ggagaaggcg
     1681 gtataaccgc gcgcgggcga aaaaaggcaa gaacggcatt cggaaaatac ggggcgtttc
     1741 gagcctcgaa gcattcccgg gccggaggag cggactttct cgacgctccc cgcccgggaa
     1801 agtatacgcg ataaggagcg tggatattag catgttgcag cgggtttttc gtttcacggc
     1861 ggcgttggcg ttggtcgggg cgctctccct tcccctcggg gcggcggagc ttccgagggc
     1921 gagcctgggg tacattttca cgacccatca ggagccgatc atggtggcga tggcgaaggg
     1981 ggaggcgttc gagcctcttg gagcatatct gaagccggtg gtggacaagg aacgctacga
     2041 tctgttcgtg gacggcgctc cggtggccag gatcagcatc gtcgtaacaa agagcggctc
     2101 ggagatggcg acgctcttcg cgcagaatca tctca
//
LOCUS       DAOPKU010000527         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_60661_length_2126_cov_3.560116, whole genome shotgun sequence.
ACCESSION   DAOPKU010000527 DAOPKU010000000
VERSION     DAOPKU010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60661_length_2126_cov_3.560116"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1330
                     /locus_tag="PK849_13140"
     CDS             323..1330
                     /locus_tag="PK849_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE67118.1"
                     /translation="MNLKRIVVWALMTALVASCAAGTAFAADTVPLEGRMTLYTSQPE
                     RDVTKLIAAFNELHPDVAVDVFRSGTEEVVSKVRAEHAVRSVLADVLLVADSVTFESL
                     KEDGMLLPYDSPEMKGIPEEYLDKDRTYAGTKIISTGIIYNTTKVKEPPVSFADLAKS
                     EYKDSIIMPSPLYSGAAAYNLGVMTRTEGLGWEFYEALKANNIRIEKGNGAILTAVVA
                     GEKACGLVVDFMTVRSKRDGAPVEFVYPLEGSPAVTEPIGILKTTEVPELAKAFVDFV
                     LSAEGQKVAAEMGYTPVKDGVEAPEGLKSVGEIKSMVYDIAVLKEAREAEKARFSAMF
                     Q"
     gene            1346..>2126
                     /locus_tag="PK849_13145"
     CDS             1346..>2126
                     /locus_tag="PK849_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPE67119.1"
                     /translation="MRVRQFAHTFGSRALKTAFYGAVFLFFIAPLLRLLLMSFAGEAG
                     YDASHYVRLLAEERTRLAIRNTAIIGTSATAFSVLFGGGFAFLVAYANLKRRRLAEVL
                     ILLPFIIPSYIITLSWTGLLSGNGAINTWLTSAGLPAVNLYSMGGIIFVHGMCATPIV
                     YMIVKAMLRRIPVDLEWASMAAGYTRRQTMLRINLALAAPAVASGAMLSFLATIDNFS
                     IPAFLGISSGIPVLSTYIYEKAIGFGPSSFSDAAVLSVLLSL"
BASE COUNT          391 a          712 c          663 g          360 t
ORIGIN      
        1 tcgaggagaa gctggggaag cgccccgaac tgctcgagat gaaccgcctc gcgctgaagc
       61 gcggaatgga gtcggcgacg ccgagaacat aagacgacgt ccgtccccgc ggacgggctc
      121 gcgcttcgcc gcgccgccgg aacgattttt ccgccgcaaa gcagtataat gttgctttgc
      181 ggttacaccc gcgcaacgct tttgttacgc gtgcgccgta cactctgcgg gaacgccttc
      241 gcctcagccc gacggcgccg gtcggaaggc aaggcgcgga acacccgcga aacgcggact
      301 cacacgcaag gaggagaact gaatgaatct gaaacgaatc gtagtctggg cgctgatgac
      361 ggccctcgtc gcatcgtgcg ccgccggaac ggctttcgcg gcggatacgg tcccgctcga
      421 agggcggatg accctgtaca cgtcccagcc ggaacgggac gtgaccaagc tcatcgcggc
      481 cttcaacgag ctgcatccgg acgtcgcggt ggacgtcttc cgctcgggaa ccgaggaggt
      541 cgtcagcaag gtccgcgcgg agcacgccgt gcggtcggtg ctcgcggacg tgctgcttgt
      601 ggccgacagc gtcaccttcg agagcctcaa ggaggacggc atgctcctcc cctacgactc
      661 ccccgagatg aagggcatcc ccgaggagta tctggacaaa gaccgcacct acgcgggaac
      721 gaaaatcatc agtaccggca tcatctacaa caccacgaag gtgaaagaac ctcctgtctc
      781 cttcgccgac ctggcgaaga gcgaatacaa ggacagcatc atcatgccca gcccgctcta
      841 ctccggcgcc gccgcgtaca acctcggcgt gatgacccgg accgaggggc tcggctggga
      901 gttctacgag gcgctgaagg cgaacaacat ccgcatcgag aaggggaacg gcgccatcct
      961 caccgcggtg gtcgcgggcg agaaggcctg cggactcgtg gtcgacttca tgaccgtccg
     1021 ctcgaaacgc gacggcgcgc cggtggagtt cgtctacccg cttgaaggct cccctgcggt
     1081 caccgagccg ataggcatct tgaagacgac ggaagttccc gagctggcga aggccttcgt
     1141 cgacttcgtc ctctcggccg aggggcagaa ggtggcggcg gagatggggt acacgcccgt
     1201 caaggacggc gttgaggccc ccgaagggct gaaatcggtg ggcgagatca agagcatggt
     1261 ctacgacatc gccgtgctga aagaggcgcg agaggccgaa aaggcgcgat tcagcgctat
     1321 gttccagtag aaaccgggga gaacggtgcg cgtacggcaa tttgcacaca ctttcggaag
     1381 ccgcgccctg aagacagcct tctacggcgc ggtcttcctc tttttcatcg cgccgctgct
     1441 ccgcctgctg ctgatgagct tcgccgggga ggcagggtac gacgcgtcgc actacgtccg
     1501 cctcctcgcc gaggagcgga cgcggctggc catcaggaac acggcgatca tcgggacgag
     1561 cgccacggcg ttttccgtac tcttcggcgg cggattcgcg ttcctcgtcg cctacgcgaa
     1621 cctgaagcgg cggcgtctcg ccgaggtgct gatcctgctc cccttcatca tcccctcgta
     1681 catcatcacg ctctcctgga cggggctgct ctcggggaac ggcgcgatca acacgtggct
     1741 gacctccgcc gggctccccg ccgtcaacct ctactccatg ggcggcatca tcttcgtcca
     1801 cggcatgtgc gcgacgccca tcgtctacat gatcgtgaaa gccatgctgc ggcgcatccc
     1861 cgtcgacctt gaatgggcct cgatggccgc ggggtacacg cgccgccaga ccatgctccg
     1921 gatcaacctc gcgctcgccg cgcccgccgt ggcgagcggg gcgatgctct ccttcctggc
     1981 caccatcgac aacttctcca tcccggcctt cctcggcatc tcctcgggca taccggtgct
     2041 gagcacctat atctatgaga aggcgatcgg cttcggcccc tcctccttct ccgacgcggc
     2101 ggtgctctcg gtgctgctct ccctcc
//
LOCUS       DAOPKU010000528         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_60675_length_2126_cov_2.765331, whole genome shotgun sequence.
ACCESSION   DAOPKU010000528 DAOPKU010000000
VERSION     DAOPKU010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60675_length_2126_cov_2.765331"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="PK849_13150"
     CDS             <1..1877
                     /locus_tag="PK849_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HPE67120.1"
                     /translation="KENDAVWYVLGPEGKMVIRLMRESVFVQMLVVLVLLFMLVFSLW
                     GFWYAGVVPLAGLFLTKGFTLGNKLFAEGREGRRRFRMKGGHAVILGWDKTGPGILRR
                     SLEENDRALVFSSLGTGNILSDMAAANIRPDKKLFLYNGNYADGDELNELSLESASRL
                     YILGDAGDPSRDSRNIETAVTILDILEKTSPSTPLDCFFQAYDLHIFNMMQGSDFFAK
                     ADRRQLNLRLFNFYEEWARRLWSFLPEDGEAAYPPLAYDPQRLSEGGNVHLVIAGFGT
                     MGQALAVQAIRICHYANGAKTRITAIDRDMERKKEEFLRCFPILDENGQCVLHDVEVN
                     CIAASLESPEIRRFLKQTAADEKQLPTVVVCFSDVDGSLSAALSLPLEIKKSRIPVLI
                     RQEGHTGLRALKRRFEESRDTEGSPLWSNIHYFGGLDEYFHDGESTERSAMLLNALHH
                     VDALSEGELDASVAEARKRGFGSLEAYFWAELDEKFRWSNRYQAAALVERLRAFGCAL
                     PYAGTRKTEVFPVNASFDGAKAKKKIEEIVRDAPFTGKRREQFAEAEHDRWWAERTLA
                     GWEYADMTDRENLKHADLRPYDELPPETRRKDLLVANASRLLTGFSGEATLGSGEGGS
                     "
     gene            1878..>2126
                     /locus_tag="PK849_13155"
     CDS             1878..>2126
                     /locus_tag="PK849_13155"
                     /inference="COORDINATES: protein motif:HMM:NF014124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HPE67121.1"
                     /translation="MELGGEMLERLAEAVHRVWMEGRLKEGWRLGDRIDKEEKIHTCL
                     VPYEQLSETDKESDRDVARGLPRILELAGYGLVRLDEDD"
BASE COUNT          459 a          612 c          692 g          363 t
ORIGIN      
        1 acaaggaaaa cgatgccgtg tggtacgtcc tcggcccgga agggaagatg gtcatcaggc
       61 tgatgcggga gagcgtgttc gttcagatgc tcgtcgtcct cgttctcctc ttcatgctgg
      121 tcttctcgct gtgggggttc tggtacgcag gggtcgtccc tctcgcgggc ctattcctga
      181 cgaagggctt taccctcggc aacaagctct tcgcggaagg cagggaggga agacgacgct
      241 tcaggatgaa gggcggtcac gccgtcatcc tggggtggga caagacggga ccgggaatac
      301 tcaggagaag cctggaggaa aacgacagag cgctcgtctt ctcctcgctc ggaaccggga
      361 acatcctctc cgatatggcg gcggcgaata tcaggccgga caagaagctc ttcctctaca
      421 acggaaacta cgccgacggg gacgaactca acgaactctc cctcgaatcg gcctcccgcc
      481 tctacatcct gggggacgcc ggagacccgt cccgcgacag cagaaacata gaaaccgcgg
      541 tcacgatcct cgacatactg gagaagacat ccccttccac gccgctggac tgcttcttcc
      601 aggcgtacga cctgcacatc ttcaacatga tgcaggggag cgacttcttt gctaaagcgg
      661 acagacgcca gctgaatctt cggctcttca acttctacga ggagtgggcc agaagactgt
      721 ggagcttttt gcccgaggat ggcgaagcgg cgtatccccc tctcgcctat gatccgcagc
      781 gcctctcgga gggcggcaac gtccacctcg tcattgccgg attcggcaca atgggacagg
      841 ccctggcggt gcaggcgatc aggatatgcc actacgcgaa cggagcgaaa acacggatca
      901 ccgcgatcga cagggatatg gagcggaaga aggaggaatt cctcagatgc ttcccgatac
      961 tcgacgagaa cgggcagtgc gtgctgcacg acgttgaagt gaactgtatc gcggcctcgc
     1021 tcgaatcccc tgaaatcagg cgattcctga aacagaccgc cgcggatgaa aagcagctcc
     1081 cgaccgtcgt cgtctgcttc tccgatgtcg acggctccct ctcggcggcg ctctcgcttc
     1141 ccctcgagat aaagaaatcc cgaattccgg tcctgatacg ccaggagggg cacaccggcc
     1201 tgcgcgccct gaagagacgc ttcgaggaga gccgcgacac cgaaggatcg cccctctgga
     1261 gcaacatcca ctatttcggc ggactggatg aatacttcca cgacggcgag agcaccgaac
     1321 gctcggcgat gctcctgaac gcgctccacc atgtggacgc cctttcggag ggcgaactgg
     1381 acgcgtccgt cgcggaggcc cggaagagag gcttcggcag cctggaggcg tacttctggg
     1441 cggagctgga cgaaaaattc cgctggtcga accgctacca ggccgcggcc cttgtggaac
     1501 ggctgcgcgc cttcggctgc gcgctgcctt acgccgggac acggaagacg gaagtgtttc
     1561 ccgtaaacgc gtcattcgac ggcgcgaagg cgaagaagaa gatcgaggaa atcgttcgcg
     1621 acgcgccctt cacggggaaa agaagagaac agttcgccga ggccgagcat gaccgctggt
     1681 gggcggagcg gacgctcgcc gggtgggaat acgccgatat gacggacagg gagaacctga
     1741 agcacgcgga ccttcgaccg tacgacgaac tgccaccgga gacgcggaga aaggatcttc
     1801 tggtggcgaa tgcttcccgc ttgctgacag gattttcggg tgaagcgacg cttgggagtg
     1861 gagaaggggg ttcgtagatg gagctcggcg gcgagatgct ggagagactc gcggaggcgg
     1921 tgcatcgggt ctggatggaa ggacgtctga aggaagggtg gcgcctcggc gaccgcatcg
     1981 acaaggagga gaagatccac acctgcctgg ttccctacga gcagctgagc gagacggaca
     2041 aggagtcgga cagggatgtg gcgcgagggc tgccgcgcat cctggaattg gcggggtacg
     2101 gcctcgtccg ccttgatgaa gatgat
//
LOCUS       DAOPKU010000529         2124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_60761_length_2124_cov_2.866119, whole genome shotgun sequence.
ACCESSION   DAOPKU010000529 DAOPKU010000000
VERSION     DAOPKU010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60761_length_2124_cov_2.866119"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PK849_13160"
     CDS             complement(<1..621)
                     /locus_tag="PK849_13160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE67122.1"
                     /translation="MNGPLLSVAIYTYNQERFVTETLDSLLESGGLDALSGGTELIVS
                     DDCSADGTAAAVSSWMERRGRCFHSCRLLRGERNEGPVRNYVRAVRACSGEIVKPLAG
                     DDLFCPEVLRDCVLKMASQQEMAIAFGRVATFSTGTCAVEREWTAEQRRFFAEDASGQ
                     FRMLASFDPLPAPGVLFRRSLFERLRLWEHDFFWMEDWPFWLLATAN"
     gene            complement(618..1631)
                     /locus_tag="PK849_13165"
     CDS             complement(618..1631)
                     /locus_tag="PK849_13165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE67123.1"
                     /translation="MLDVRNQGSARPMILYAGLGSDYGRPGLGLGYDTVHFYYSLVGG
                     GYPVLHFPYDRVRADLGHERMQSALRLACMLFRPSILLHAILDDEFSAETIRAISSEL
                     GIPTVAFFSDDHWRFDDFSRFAARPYDWVCTTSADAAGRYEELGFTNVIRSQWGANHF
                     FFRPMSCGYEYDVSFIGQPHGCRREVVRRLRERGVRIAVRGRGWEEGRIGLSEMIRMF
                     SASRINLNLSNASVSESLEKDGERPLLQIKGRDFEVPACGGFLLTQENPELNDYFEPG
                     KEIAVYRDTDDLLEKIEYYLAHDEERERVRKAGYDRFLKEHTMLSRLETIFSRVLSGE
                     RAE"
     gene            complement(1761..>2124)
                     /locus_tag="PK849_13170"
     CDS             complement(1761..>2124)
                     /locus_tag="PK849_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67124.1"
                     /translation="TQTDPRRWFEWATLVCAGSTSAVEAVLAHRPTVVFSGYWIGLLT
                     PETLDEAFASYFGERSGPYYVRHHLEKISAEIQRVYDEWESERMERDLAAARAAVEPR
                     FRREGAATEFERLFRSLA"
BASE COUNT          421 a          719 c          608 g          376 t
ORIGIN      
        1 gttcgccgtc gccagaagcc agaagggcca gtcttccatc cagaagaaat cgtgctccca
       61 gagtcgaagg cgctcgaaca gggaacggcg gaagagaact cccggcgccg gcagagggtc
      121 gaacgacgcc agcatacgga actgcccgga cgcatcctcc gcgaaaaaac gccgctgctc
      181 cgcggtccac tctctttcga cggcgcatgt ccccgtcgaa aacgtcgcca ccctgccgaa
      241 cgcgatcgcc atttcctgct gcgaggccat cttcagcacg cagtcgcgca gaacctccgg
      301 acagaagagg tcgtcccccg ccagcggttt gacgatctcg ccggagcagg cgcgcacagc
      361 tcgtacatag ttgcgcacag gaccttcgtt cctttcgccc cgaagaagac ggcacgaatg
      421 aaaacatctc ccccgccgct ccatccatga ggagacggcg gcggccgttc catccgctga
      481 gcagtcgtcc gatacgatca gctccgttcc tcccgaaagg gcgtcaagcc cgccggactc
      541 aaggaggctg tcgagcgtct ccgtcacaaa gcgctcctgg ttatatgtat agatcgccac
      601 cgagagaagc ggtccgttca ctctgcccgc tcccccgaga gcaccctgga gaagatggtt
      661 tcaagacgcg agagcatggt gtgctccttc aaaaagcgat cgtaccccgc tttccgcacg
      721 cgctcgcgct cctcgtcgtg cgcgagatag tactctatct tttcgagaag gtcgtcggta
      781 tcgcggtaga ctgcgatctc cttccccggt tcgaagtaat cgttcagctc cggattctcc
      841 tgcgtcagca ggaaacctcc gcacgcgggc acttcgaaat ccctcccctt gatctgcagc
      901 agcggccgtt cgccgtcctt ttcgagcgat tccgagacgg aggcgttgct caggttcagg
      961 ttgatgcggc ttgcgctgaa catgcggatc atctcgctca ggccgattcg tccctcttcc
     1021 cacccgcggc cgcgcacggc gatccggacg cctcgttcgc gaagccgccg gacgacctcc
     1081 cgcctgcacc cgtgaggctg cccgatgaag gaaacgtcgt actcgtatcc gcacgacatc
     1141 gggcggaaga aaaagtggtt cgccccccac tggctacgga tgacgttcgt gaacccgagc
     1201 tcctcgtacc gaccggcggc atccgcgctg gtggtgcata cccagtcgta cgggcgcgcg
     1261 gcgaaacggg aaaagtcgtc gaagcgccag tgatcgtcgc tgaagaaggc caccgtcgga
     1321 atcccaagct ccgatgaaat cgcacgaatg gtctccgcgc tgaattcgtc gtcgagaatc
     1381 gcatggagga gtatcgacgg cctgaagagc atgcaggcaa gccgaagcgc cgactgcatg
     1441 cgttcgtgcc cgaggtccgc ccgcaccctg tcgtacggaa aatggaggac cgggtaccct
     1501 ccgccgacca gcgagtagta gaagtgcacg gtgtcgtacc ccaggccgag ccccggacgt
     1561 ccgtagtccg agccgagacc ggcgtagagg atcatcggac gggcacttcc ttgattcctg
     1621 acgtcgagca tggaatggac gtactcctcg tcgctccagg atggccatac cctgtttttt
     1681 gccgcgcgca gcctctttcg aaaatccttc aatccgatga ccggtcacct ccctcgaaga
     1741 atgcgtccga gaacgccaaa tcaggcaaga ctccggaaga gccgctcgaa ttccgtggcc
     1801 gcgccctccc gcctgaaacg gggctccacc gcggcgcgcg ccgcagcgag gtcgcgttcc
     1861 atcctctcgc tctcccattc gtcgtagacc ctctgtatct ccgcagaaat cttctccaga
     1921 tgatgccgga catagtacgg accgctccgc tccccgaagt aggacgcaaa cgcttcgtcg
     1981 agcgtttccg gcgtgagcaa tcctatccag tatccggaga agaccacggt cgggcgatgc
     2041 gccagcactg cttcgaccgc cgacgtggac ccggcgcaga cgagcgtcgc ccattcgaac
     2101 cagcgccgag gatcggtctg cgtc
//
LOCUS       DAOPKU010000530         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_61382_length_2108_cov_5.219679, whole genome shotgun sequence.
ACCESSION   DAOPKU010000530 DAOPKU010000000
VERSION     DAOPKU010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61382_length_2108_cov_5.219679"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="PK849_13175"
     CDS             complement(<1..1605)
                     /locus_tag="PK849_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPE67125.1"
                     /translation="MAETSRKSGRQMLMFAPRGEATDLALMVDALGHEETHPWNAERI
                     LEALVKEAGVEPGLAGEIAAEVESDLLRWGRDRVSTSLIRELVNVKLFQRGLDAKLQD
                     HSRIGIPVHDLETMMLHPNRENSNTTLNPESINLSVAELALKQYALTRVFSGDVAEAH
                     LSGNIHLHDLGMVNRPYCSGQSIAYVAKYGLNLPSITSISAPARHPDVLLAHLLKMTS
                     VLQNNFAGAIGWDAVNTFFAPYLEGLSDERIRQIAQMLIFEFNQLAGGRGGQVAFTDV
                     NLYYEVPKHFRDVPAIGPGGKATGKTYGDYASEAKRFLRALFSVYMEGDSRGQPFFFP
                     KPLLHITEDFFREDGWEEMLSLACEVASEKGNTYFVFDRGEAVKLSECCRLSFELDHR
                     DILEARTPWKLRFCALQNVTLNLPRMAYRSGGDRETLFELVDHYMEIAAKAHLQKKKF
                     ISEILDLGKRGPLSVLCMEHDGEPYLRFERVKYLVGILGMNEMVQTITGRQLHESEEA
                     FQLALAVTQYMNLKCQALSERYGLAIV"
     gene            complement(1637..>2108)
                     /gene="nrdR"
                     /locus_tag="PK849_13180"
     CDS             complement(1637..>2108)
                     /gene="nrdR"
                     /locus_tag="PK849_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPE67126.1"
                     /translation="TRVIETRTADEGRVVRRRRECPECSTRFTTYEKIEEKRPLRVIK
                     KDGSRETFGRDKIVRGISRACEKLPVSLEQIEDLATRIETDLKAEGYGDIPVSLIGQR
                     VMEGLKRISQVAYVRFASVYREFTDIESFQHEIARLIGEREKREGSGNGEGSSE"
BASE COUNT          454 a          650 c          524 g          480 t
ORIGIN      
        1 cacgatagcg agtccatagc gttccgaaag agcctggcat ttcaaattca tgtactgcgt
       61 aaccgccagc gcgagctgga acgcctcttc cgattcgtga agctgacggc ccgtgatcgt
      121 ctggaccatc tcgttcatac ctaatatacc tacaaggtac ttcaccctct cgaaccgaag
      181 atacggttcg ccgtcgtgtt ccatacagag aacagaaagc gggcctcgct tcccaagatc
      241 aagtatctca ctgatgaatt ttttcttctg caggtgcgct ttcgcggcaa tctccatgta
      301 atggtctacc agctcgaaga gcgtctccct gtctccgccg ctcctgtacg ccattctggg
      361 gaggttgagc gtcacgttct gcagggcgca gaagcgaagc ttccacggtg ttctcgcttc
      421 caaaatatcc ctgtgatcaa gctcgaagct taaacggcag cactcggaga gcttgacagc
      481 ctctccccgg tcgaagacaa aataggtgtt tcccttttcg gacgcgacct cgcaggcaag
      541 ggagagcatc tcctcccatc cgtcctcgcg gaaaaagtcc tctgtgatgt gcagcagtgg
      601 ctttgggaag aaaaagggct gcccccggct gtcgccctcc atgtacaccg aaaagagcgc
      661 tctcaggaac cgcttcgctt cggaagcata gtcgccgtat gtcttccctg tcgccttgcc
      721 tcccggaccg atcgccggaa catcccgaaa gtgcttcggc acctcgtagt acaggttcac
      781 atccgtgaag gccacctgac cgccgcgccc tcctgcgagc tgattgaact cgaagatgag
      841 catctgggca atctgacgga tacgctcatc cgaaagacct tcaagatacg gagcaaaaaa
      901 agtgttcaca gcatcccaac cgatcgcccc cgcaaaattg ttctgcagta cggaggtcat
      961 cttgagaaga tgtgcgagca gcacgtccgg gtgacgcgca ggagcggata tgctcgtgat
     1021 agaggggaga ttcagaccat acttcgccac ataggcgatg ctctgcccgg aacagtaggg
     1081 acggttcacc ataccgagat catgaaggtg gatattcccc gaaagatgcg cctcggcgac
     1141 atctccggaa aagacgcgcg taagggcgta ctgcttcaga gccagttcag ccaccgacag
     1201 gttgattgac tcgggattga gagtggtatt gctgttctcc ctgttcggat gaagcatcat
     1261 ggtttcaaga tcgtgcaccg gaattcctat ccggctgtgg tcttgcagct ttgcgtccaa
     1321 tccgcgctga aaaagcttga cgttcaccag ttcgcgaata agcgacgtgc tcacccggtc
     1381 gcgtccccaa cgcaagaggt ccgactccac ttccgccgcg atctctccgg caagcccggg
     1441 ctcaactccg gcttccttca caagagcttc gagaatccgc tccgcattcc aggggtgcgt
     1501 ttcctcatgc ccaagagcgt caaccataag cgcaagatcg gtcgcctcac cccgaggagc
     1561 gaacatgagc atctgccgtc cgcttttcct ggatgtctcg gccatatcgc ccttgggttt
     1621 cagcagcacc ggagtatcat tcagacgatc cttctccatt gccggaaccc tcccttttct
     1681 cccgttcgcc gatgagccgg gcaatttcgt gctggaagct ttcaatatcc gtaaactccc
     1741 tgtagaccga ggcgaaacgg acataagcta cctgactgat tcgcttcagt ccctccataa
     1801 ctctctgccc tatgagggac acggggatgt ctccgtaccc ttcagctttc aggtcggttt
     1861 caatacgggt cgccagatcc tcgatttgtt caagggaaac aggaagcttc tcacatgccc
     1921 ggcttattcc cctgacaatc ttgtcccgac cgaacgtctc cctgctgccg tcctttttta
     1981 tcacccggag aggacgtttc tcctcaattt tttcatatgt tgtgaaccga gtggagcact
     2041 ccggacactc cctcctccgg cggaccacac gcccttcatc cgcagtcctc gtctcgatta
     2101 cccgcgtc
//
LOCUS       DAOPKU010000531         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_61384_length_2108_cov_4.773989, whole genome shotgun sequence.
ACCESSION   DAOPKU010000531 DAOPKU010000000
VERSION     DAOPKU010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61384_length_2108_cov_4.773989"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PK849_13185"
     CDS             <1..892
                     /locus_tag="PK849_13185"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HPE67127.1"
                     /translation="LVHKAPPEESVSAMGAKAALRALEGIDPASLDLVVYCGSEYKDY
                     YLYNCAAKIQHEIGAVNANAFEIHSLCSAGVYSLKVLKGMMLADDDLKRVLLVSSSKE
                     GDLINYADGESRFMFNFGDGAAAAVLEKGLARNVVLETAMITDGSFADDIGVMGIGCK
                     NFAGFSDMPYGDRFLKVADIARMKERLDPVTLDNFVRVIERAAEKSGYAKDAIGFIAP
                     IFMKRSILETMLASFGLSEENSFVLEDYGHCQSADAFIALQNGESLGRLKEGDVAVLV
                     AAGTGYTWAATAVKWGAWSA"
     gene            948..1364
                     /locus_tag="PK849_13190"
     CDS             948..1364
                     /locus_tag="PK849_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPE67128.1"
                     /translation="MKGKTIKEIHAGDTASFQKTLSESDVYLFAGVSGDQNPAHINET
                     YASGTRFKTRIAHGMLVASLISAALGMELPGPGTIYLGQTIKFTAPAHIGDTVEARVT
                     VTEIDERRNIVTLETVCTNQDGKVLLEGVATVMPPK"
     gene            1378..>2108
                     /locus_tag="PK849_13195"
     CDS             1378..>2108
                     /locus_tag="PK849_13195"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPE67129.1"
                     /translation="MSERIVLAGAVRTPIGKFGGTLAEVPVEKLGALVIKEALRRAGV
                     APGQVDEVLMGCILQACRGQNVARQAAIGAGLPVEVPATTLNNVCGSGLKAVNLAADM
                     IRAGSADVVVAGGMENMSAAPYALEKARSGYRMGDGRCVDTMVLDALTDAFNNYHMGV
                     TAENVAEKYGITREMQDEFAAESQRKCEAAWNAGRFDEEIVPVPVTVKKQTVLFERDE
                     HLRAGVSAEDLASLKPAFKKEGGTVT"
BASE COUNT          407 a          623 c          759 g          319 t
ORIGIN      
        1 cctggtgcac aaggccccgc cggaggagtc cgtctccgcg atgggcgcga aggcggcgct
       61 gagggcgctg gagggaatcg acccggcgtc gctcgacctg gtggtctact gcggcagcga
      121 gtacaaggac tactacctct acaactgcgc ggcaaagatc cagcacgaga tcggggcggt
      181 caacgcgaac gccttcgaaa ttcacagcct ctgttcggcg ggggtctatt cgctcaaggt
      241 actgaagggc atgatgcttg cggacgacga tctgaagcgc gtcctgctgg tctcctcctc
      301 caaggagggg gacctgatca actacgccga cggcgaatcg cgcttcatgt tcaacttcgg
      361 cgacggcgcg gccgcggcgg tgctcgaaaa ggggttggcg cggaacgtcg tgctggagac
      421 cgccatgatt accgacggaa gcttcgcgga cgacatcggc gttatgggga tcgggtgcaa
      481 gaacttcgcc ggcttcagcg acatgccgta cggcgaccgc tttttgaagg tcgcggacat
      541 cgcccggatg aaggagcggc tcgatccggt cacgctggac aacttcgtcc gggtgatcga
      601 acgggcggcg gagaagagcg ggtacgccaa ggacgccatc ggcttcatcg cccccatctt
      661 catgaagcgc tccatcctcg agaccatgct cgccagcttc gggctgagcg aggagaactc
      721 gttcgtgctg gaggactacg gccactgtca gtcggccgac gcgttcatcg ccctccagaa
      781 cggagagtcg ctggggaggc tgaaggaagg ggacgtcgcc gtcctcgtcg cggcgggcac
      841 ggggtacaca tgggccgcta cggcggtgaa gtggggcgcg tggagcgcgt agccggagga
      901 ggcacggagg agaagccccc cgtccggggg cgccgaagga ggacgctatg aagggaaaga
      961 caatcaagga gatccatgca ggcgacacgg cttcgttcca gaagacgctg agcgagtcgg
     1021 acgtgtacct cttcgccggg gtgagcggag atcagaatcc ggcccatatc aacgaaacgt
     1081 acgcctccgg cacgcgattc aagacccgca tcgcccacgg tatgctcgtc gcctcgctga
     1141 tctccgccgc tctcgggatg gagctgcccg ggccggggac catctacctc gggcagacga
     1201 tcaaattcac agcccccgcc catatcggcg acacggtgga ggcgagggtg accgtcacgg
     1261 agatcgacga gcggaggaac atcgtcacgc tggagaccgt ctgcacgaat caggacggga
     1321 aggtgctgct cgaaggcgtc gcgaccgtga tgccgccgaa gtgagggagg gaatgcgatg
     1381 agcgaacgga tagtgctggc gggcgcggtg cgcaccccga tagggaagtt cggcgggacg
     1441 ctcgccgagg tccccgtcga aaagctcggc gcgctggtga tcaaagaagc gcttcggcgc
     1501 gcgggcgttg cgcccggtca ggtggacgaa gtcctgatgg ggtgcatcct tcaggcctgc
     1561 cgcgggcaga acgtggcgcg gcaggcggcg atcggcgcgg ggctccccgt ggaggttccc
     1621 gcgacgacgc tgaacaacgt ctgcggctcc ggcctcaagg cggtgaacct ggctgcggac
     1681 atgatccgcg ccgggagcgc cgacgtcgtc gtggcgggag gcatggagaa catgtctgcg
     1741 gctccctacg cgctggagaa ggcgcggtcc ggctaccgca tgggcgacgg ccgctgcgtg
     1801 gacaccatgg tgctcgacgc gctgacggac gccttcaata actaccacat gggcgtgacg
     1861 gccgagaatg tggccgaaaa gtacggaatc acccgcgaga tgcaggacga gttcgccgcc
     1921 gaaagccagc ggaagtgcga ggcggcgtgg aatgccgggc ggtttgacga ggagatcgtc
     1981 cccgttccgg taacggtcaa gaagcagacc gtcctcttcg agcgcgacga acacctccga
     2041 gcgggcgtct ccgctgagga tcttgcgtcg ctgaagccgg ccttcaaaaa ggaggggggg
     2101 acggtgac
//
LOCUS       DAOPKU010000532         2107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_61432_length_2107_cov_4.513158, whole genome shotgun sequence.
ACCESSION   DAOPKU010000532 DAOPKU010000000
VERSION     DAOPKU010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61432_length_2107_cov_4.513158"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK849_13200"
     CDS             <1..284
                     /locus_tag="PK849_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPE67130.1"
                     /translation="TMVVSSLLSAVMVALLGVIGFVGLVCPHMLRRFLGSDHRFLIPG
                     SVLMGGLLLLAADTGARLAFAPRTLPVSVFTAFLGAPVFLALIARRKRT"
     gene            281..1033
                     /locus_tag="PK849_13205"
     CDS             281..1033
                     /locus_tag="PK849_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE67131.1"
                     /translation="MILSVNGISFAYRGVPVLDGVTFSLAEGEMVALLGPNGTGKTTL
                     LRTINGILRPKGGTVLVESRDASTCSPRERAKCFGYVPQRGEAVRLTVFDAVLLGRRP
                     HLGWTVERRDLEKVESVLETLSLEKFTLRYLDELSGGEFQKVLLARALVQEPRVLLLD
                     EPTSSLDLKNQLDMLATLQRVVRGHNVAALLSLHDLNTAFRFADRLLFLKDGVIRGSC
                     APSDAPEALIREVYDVPVDIVSHKGIPMIVPS"
     gene            1301..2044
                     /locus_tag="PK849_13210"
     CDS             1301..2044
                     /locus_tag="PK849_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE67132.1"
                     /translation="MFRSLAAVCLWIFLLLPAPETARAGERVFRISTSYKSLLSTPEQ
                     TGMLDMLLKEAFRRSGLRAEIVFTPTERSMYDVDAGLLDGEINRIEGMEKRFPNLVRV
                     PEPNMTMHFVAFSNRPYPVDGWESLKNLHVGVVSGWRILEEHTVDFPKRILVPSETEL
                     FTMLHKGRIDVALYDKLTGYETISRLGFPEIRHLEPPLINRDMFLYMHKSHSEVVGTI
                     AEALRGMKLDGAYDRIVSEAIASLGADAR"
BASE COUNT          408 a          653 c          625 g          421 t
ORIGIN      
        1 aaacgatggt agtctcctcg ctcctctcgg cggtaatggt ggccctgctc ggcgtgatcg
       61 gcttcgtcgg actggtctgt ccgcacatgc tccgccgctt tctgggctcg gaccacagat
      121 ttcttatccc cggctcggtg ctgatggggg ggctgctcct gctggcggcg gacacggggg
      181 cgcgtctcgc cttcgcgccg cgcacgctcc cggtgtcggt cttcacggcg tttctgggcg
      241 ctccggtctt cctcgcgctc atcgcacgga ggaagcggac atgatcctct cggtgaacgg
      301 aatctccttc gcctaccgtg gcgtgccggt actggacggg gtgactttct cgctcgccga
      361 gggagagatg gttgcgctcc tcggcccgaa cggaacgggg aagacgacgc tgctacggac
      421 gatcaacggc atcctccgcc ctaagggggg aacggtcctc gtcgaatcga gggacgcctc
      481 tacatgctcc ccgcgcgaac gggcaaagtg cttcggctac gttccgcagc ggggcgaggc
      541 cgtgcgcctg acggtcttcg acgccgtgct gctcggccgc cgcccgcacc tggggtggac
      601 cgtggaacga cgagacctcg aaaaggtaga gtcggtcctc gaaacgctct cgctcgaaaa
      661 gttcaccctc cgctacctcg acgaactcag cggcggcgag ttccagaagg tgctcctcgc
      721 ccgggcgctc gtccaggagc cgcgggtact cttgctcgac gaaccgacga gtagccttga
      781 cctgaagaac cagctcgaca tgctcgccac gctgcaacgg gtcgtccgtg gccacaacgt
      841 cgccgcgctg ctgagcctgc acgacctgaa caccgccttc cgcttcgccg accgcctgct
      901 ctttttgaag gacggcgtca tccggggctc ctgcgcgccg tcggacgctc ccgaggcgct
      961 gatccgcgag gtctacgacg tcccggtgga catagtctcc cacaagggca tcccaatgat
     1021 cgtaccgtca tgagcacaac acggaaataa aggcgaaagc ctgtcttcaa caccccatat
     1081 acggagcaac caaacggacg ggcttgaaaa gtcgacggaa acatgtcgat tgttttcaag
     1141 cccgtccgcc tacaaattat tcttttttcg gagaagcact cgattgaaat tgtatttacg
     1201 gggagttcat ggtaaaaata gacctgcctt ctccgcttcc gtccttgttt tccccgtacg
     1261 gggccggccg gcggagtatc aacgacaaag gggtgacgtc atgttcaggt ctcttgcagc
     1321 ggtctgcctc tggatttttc ttttactccc ggctccggaa acagccaggg cgggtgagcg
     1381 tgtctttcgc atcagcacat cgtataaaag cctgctctcc accccggaac agaccggaat
     1441 gctggatatg ctgctcaagg aggccttccg gaggtccggc ctgcgggcgg agatcgtctt
     1501 cacgcccacg gagcgctcca tgtacgacgt cgacgcggga ctgctggacg gagagatcaa
     1561 ccggatcgag gggatggaga aacgattccc caatctggtg cgggtacccg aacccaacat
     1621 gaccatgcac ttcgtcgcct tctccaatcg gccgtatccc gtggacggat gggagagcct
     1681 taaaaatctg cacgtcggcg tcgtcagcgg atggcgcatt ctcgaagaac ataccgtgga
     1741 cttcccgaaa agaatcctcg ttccgagcga gacggaactc ttcaccatgc tccacaaggg
     1801 gaggatcgac gtcgctctct acgacaaact gacagggtac gaaacgatct cccgtctggg
     1861 ttttccggag atccgccatc tggagcctcc cctgatcaac agggatatgt ttctctatat
     1921 gcacaagagc cattccgaag tagtgggaac gatcgccgaa gcgctgcgcg ggatgaagct
     1981 cgacggcgcg tacgatcgca tcgtctcgga ggcgatcgcc agcctgggcg cggacgcccg
     2041 ttgaaaggac gaattccgtt gcactggaag gattcgagca cgtggaagct atttaaatat
     2101 tccttac
//
LOCUS       DAOPKU010000533         2104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_61549_length_2104_cov_4.292338, whole genome shotgun sequence.
ACCESSION   DAOPKU010000533 DAOPKU010000000
VERSION     DAOPKU010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61549_length_2104_cov_4.292338"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PK849_13215"
     CDS             complement(<1..614)
                     /locus_tag="PK849_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE67133.1"
                     /translation="MPLLKNVPELRTKPMRDVIYEHLRKAIIRGELKADATFTDGEIA
                     EEYGVSRTPVREAVQKLEAEGYIERVPMKGNRICSVSPYELANSFAIRKALETLAVRY
                     AAIRITDEELDAMRDLLAQADKAFAEFSDNELLDRFFPIVKKFNKIAFEACRSERLAE
                     LVWAQRELFDRYMVMRIILPNRINKSLSRRKLLCEALAAHDPERA"
     gene            772..>2104
                     /locus_tag="PK849_13220"
     CDS             772..>2104
                     /locus_tag="PK849_13220"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPE67134.1"
                     /translation="MGKNLVWKVIGEHLAGEAPEGGEVSLKIDHTLTQDTTGTMSYLQ
                     FEAIGVPRTRAERSVSYVDHNTLQATFENADDHNYLQDVAAKYGLLFSRPGNGICHQV
                     HLERFGVPGKTLLGSDSHTPTGGALGMLAMGAGGLDVALAMAGVPFSIPRPKVLKVEL
                     KGRLRPWVAAKDVILEVLRVLTVKGGVGKILEYCGDGVSTLTVPERGTITNMGAELGA
                     TTSIFPSDEQTRRFLEAQGRGTDFRPLAADGDAEYDETLVIDLSTLEPLIALPHSPDK
                     VVPVREVAGMPVDQCFIGSCTNSSYLDLARTAEILHGRRVAERTSLCIAPGSRQTLLM
                     ITKNGILTDFITAGARLLESACGPCIGIGQAPCSGGVSLRTSNRNFEGRSGTKDALVH
                     LVSAETAAVSALRGVITDPRTLGEAVEVRLPEKYIVDDSMVLLPPEDGSSVE"
BASE COUNT          385 a          605 c          691 g          423 t
ORIGIN      
        1 gctctctccg ggtcgtgcgc ggcgagcgct tcgcagagca gtttccgtcg ggaaaggctc
       61 ttgttgatcc tgttgggaag gatgatccgc ataaccatgt agcggtcgaa gagttcccgt
      121 tgagcccaga cgagctcggc aagccgttcg gacctgcacg cctcgaaggc gatcttgttg
      181 aatttcttca cgatgggaaa gaaacggtcg agcaattcat tatcgctgaa ctcggcgaag
      241 gccttgtcag cctgcgcaag aagatcgcgc attgcgtcca gctcctcgtc ggtgatgcgg
      301 atggcggcgt agcgcaccgc aagcgtttcg agggctttgc gtatggcgaa ggagttcgcc
      361 agttcataag gagaaacgga gcagattcgg tttcccttca tcggaacccg ttcgatgtac
      421 ccttcggctt ccagtttttg gaccgcctcc cggaccgggg tgcggctgac gccgtactct
      481 tcggcgatct cgccgtcggt gaacgtcgcg tccgctttca gttctccgcg gatgatcgcc
      541 ttccggaggt gctcgtagat gacgtcgcgc atgggctttg tacgcagttc cgggacattc
      601 ttcaagagcg gcacgcgatc atctcctcga aagttttttt gtttttttcc aataaaagtt
      661 ggatccacta tccaacatcc agcatccatg tcgtataata gcccctggaa gcaaggctgt
      721 aaagtgggtt aatagactgt attttgtata tttgcacgag ggggcggaga gatgggcaag
      781 aatcttgtct ggaaggtgat cggggaacat ctcgcaggag aggcgccgga aggcggcgaa
      841 gtttcgctga agatcgacca cacgttgacg caggatacga cggggaccat gtcgtatctc
      901 cagttcgagg cgataggcgt tccgcggaca cgcgcggagc gttcggtgag ctatgtcgac
      961 cacaacacgc tgcaggcgac gttcgagaac gccgacgacc acaactacct gcaggacgtc
     1021 gcggcgaaat acgggctgct cttctccagg ccggggaatg gcatctgcca tcaggtgcat
     1081 cttgaacggt tcggcgttcc cggcaagacg ctcctcggct ccgacagcca cactcccact
     1141 gggggcgccc tggggatgct cgccatgggc gcgggcgggc tggacgtggc ccttgcgatg
     1201 gcgggcgttc cgttctccat cccccggccg aaggtgctca aggtggagtt gaaggggcgt
     1261 ctccgtccgt gggttgcggc gaaggacgtc attctggagg tgctccgcgt tctcaccgta
     1321 aaggggggcg tcggaaaaat ccttgagtat tgcggcgatg gtgtttcgac gctcactgtc
     1381 ccggagcgcg ggacgatcac gaacatgggg gcggagctcg gcgcgacgac gtccatcttc
     1441 ccgagcgacg agcagaccag gcgtttcctt gaggcgcagg ggagaggaac ggacttcagg
     1501 ccccttgccg ccgatggaga cgcggagtac gacgagacgc tcgtcatcga cctgtcgacg
     1561 ctcgaaccgc tcattgcgct gccgcactcg cccgacaagg tggtcccggt gcgcgaggtc
     1621 gccggcatgc ccgtcgacca gtgcttcatc ggaagctgta cgaactcttc ctacctcgac
     1681 ctcgcgcgga ctgcggagat tctgcacgga cggcgcgtcg cggagcgcac cagcctctgc
     1741 atcgctccag gctcgcgcca gacgctgttg atgattacga agaacggtat tctgacggac
     1801 ttcataactg cgggcgcgcg actgcttgag agcgcatgcg gcccgtgcat cggcatcggc
     1861 caggcgccgt gcagcggcgg cgtgtcgctg cggacgtcca accggaattt cgaggggcgc
     1921 tccggcacga aggacgcgct ggtacatctg gtgagcgcgg agacggccgc ggtttcggcg
     1981 ctccggggcg tgatcaccga tccgaggacg ctcggcgagg ccgtcgaggt gcggctgccc
     2041 gagaagtata tcgtcgacga ttcgatggtg ctccttcctc cggaggacgg cagttccgtc
     2101 gaga
//
LOCUS       DAOPKU010000534         2104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_61571_length_2104_cov_2.682284, whole genome shotgun sequence.
ACCESSION   DAOPKU010000534 DAOPKU010000000
VERSION     DAOPKU010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61571_length_2104_cov_2.682284"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PK849_13225"
     CDS             complement(<1..1102)
                     /locus_tag="PK849_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE67135.1"
                     /translation="MKRKDENTAHTPAPGGNSLSSMPIIQSLGRRIEPEDSQGSSGIA
                     SRRDIVDEMAVVLNSCERRNIILLGKAGTGKTTLLRQLATRIEEGTAGKKLEGAVLVE
                     LDISLMKSEGCIAGGIEKKIRHMWAEAENFGNVVFFMDEGHKTMSGGSDSIGNILKPL
                     VTSGRVPVILATTDDEYVSIERDAAMKRRFSPVHVPEPDRDTLLKILAHKGAVYAKDS
                     DVAIGRGVCEKTLELAKRYIHDSADPDRSLRVLEKACSIASLEGGKRQVTAQHLLRAV
                     AMITNASALSEEEECGREQILKALEKTVKGQETALKKTADFLVRVNTGFHFKGRPKGV
                     LLLTGPAGCGKTLLAESAGAVLGFKPDETVRIN"
     gene            complement(1119..1697)
                     /gene="clpP"
                     /locus_tag="PK849_13230"
     CDS             complement(1119..1697)
                     /gene="clpP"
                     /locus_tag="PK849_13230"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPE67136.1"
                     /translation="MIVPHVIEQSGRGERAYDIYSRLLKDRIVFLGEGVDEHTGNIVV
                     AQLLFLESEDPKKDIHLYINSPGGYVSAGLAIYDTMQYVKCDVSTICIGQAASMGALL
                     LAGGAPGKRFSLPNSRIMIHQPLGGIQGQATEVEIHAREILKLRDRLNDIMVKHTGQP
                     KEKIAADTERDYYLSATDAMKYGIIDRVIETQ"
     gene            1888..>2104
                     /locus_tag="PK849_13235"
     CDS             1888..>2104
                     /locus_tag="PK849_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67137.1"
                     /translation="MRSWDEIAEILSRYADKTDRSSLFLGFFSEAYQGISGKNPVLVG
                     GGAVEVYTHGGYVTGDLDLICDAEIARK"
BASE COUNT          393 a          606 c          593 g          512 t
ORIGIN      
        1 ggttgatccg taccgtttca tcgggcttga aaccgagcac cgctcccgcc gattccgcaa
       61 gaagcgtctt gccgcagcct gcggggccgg tgagaagcag aacgcccttc ggccttccct
      121 tgaaatgaaa gccggtattc actcgaacga ggaagtccgc ggtcttcttc agagcggttt
      181 cctgcccctt cacggtcttt tcgagagcct tcagaatctg ctcacggccg cattcctctt
      241 cctcggaaag agcggaagcg ttggtaatca tggcgaccgc gcgaaggaga tgctgcgccg
      301 tcacctgacg ctttccgccc tcaagcgaag cgatggagca ggctttttcc agcactctga
      361 ggcttctgtc cgggtcggcg ctgtcgtgaa tgtagcgttt cgccagctcg agcgtttttt
      421 cgcagacgcc ccgtccgatg gcaacgtccg aatctttcgc gtagaccgct cccttatggg
      481 cgaggatctt cagcagggtg tcccgatccg gctcaggcac gtgcaccgga ctgaagcggc
      541 gcttcatcgc ggcgtctctt tcgatggaaa cgtattcgtc gtcggttgtc gccagaatca
      601 ccggcaccct tccgctggtg acgaggggct tcaggatgtt ccctatgctg tcggagccgc
      661 ccgacatggt cttgtgcccc tcgtccatga agaagacgac gttgccgaaa ttctccgcct
      721 ccgcccacat atgccgtatc tttttctcga tgccgcctgc gatgcagccc tcgctcttca
      781 tgagactgat gtcgagttcg acgaggacgg ccccttcgag tttctttccc gccgttcctt
      841 cttctatgcg ggttgcgagc tgacgcaaaa gggtggtttt gcccgtgccg gcctttccaa
      901 gaaggatgat gttcctccgc tcgcaggagt taagaacgac cgccatctcg tcgacaatat
      961 cccggcggga ggcgatgccc gacgaacctt gggagtcttc gggttcgatc cgtctcccca
     1021 gactctggat gatgggcatg gaagagagcg aatttccgcc cggtgcggga gtatgcgcgg
     1081 tgttttcgtc cttccgtttc acggcggccc cgtcctttct actgcgtctc gatgacgcga
     1141 tcgataatgc cgtacttcat cgcgtcggtc gcggaaagat agtagtcgcg ctcggtgtcg
     1201 gcggcgatct tctctttcgg ctgcccggta tgtttgacca tgatgtcgtt cagacggtcg
     1261 cggagcttca gaatctcgcg ggcgtggatc tccacctcgg tcgcctgacc ctgtatcccg
     1321 cccagcggct ggtggatcat gatgcgcgag ttcggcaggg agaaccgttt tcccggcgcc
     1381 cctcctgcga gtagaagcgc tcccatgctt gcggcctggc cgatgcagat ggtggagacg
     1441 tcgcacttga cgtactgcat ggtgtcgtag atcgccagcc ctgcggaaac gtaccctccc
     1501 ggactgttga tgtacaaatg gatatccttc ttcggatcct cgctctcaag aaagagcagc
     1561 tgcgccacca cgatattccc cgtatgctcg tccacgcctt cgccgagaaa gacgattctg
     1621 tccttcaaaa gacggctgta aatgtcgtac gccctctcac cgcgtccgct ttgttcaata
     1681 acatgcggta caatcatatg tggcgcctcc tctgtttaat tgaaatgtgt ttccggcagc
     1741 tgttcgcgtt ttcgataaag agcacagcca ttcggttaag aattagaggt atatagcgta
     1801 atgagattaa aacgcaatct attcatgttc gcaacaatat cacgcttttc caatcctgtc
     1861 aagggtgaac aaaaggaggt gttttctgtg agaagttggg acgaaatagc cgagatcctt
     1921 tcacgctatg ccgacaagac ggatcggtcg tctcttttcc tgggtttttt cagcgaggcg
     1981 taccaaggca tctccggaaa gaatccggtg cttgtcggcg gcggggcggt tgaagtgtat
     2041 actcatggag gctacgtcac tggcgatctg gatctgatct gcgatgcgga gatagcgcga
     2101 aaga
//
LOCUS       DAOPKU010000535         2103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_61590_length_2103_cov_3.323242, whole genome shotgun sequence.
ACCESSION   DAOPKU010000535 DAOPKU010000000
VERSION     DAOPKU010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61590_length_2103_cov_3.323242"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..933
                     /locus_tag="PK849_13240"
     CDS             454..933
                     /locus_tag="PK849_13240"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPE67138.1"
                     /translation="MTRRFLAIRPNDGNMPLNVFPSVDRMMRDMMRVFGDFSSDVGYP
                     ADAEKMLAIPRGDFYRKDGKIFAEFEIPGIDPSKVELNVYEDRLELRAEKSDEKTLEQ
                     NNVLRSERYFGTVSRSIQFPVEVDPDSAKASFKNGVLTVEVAERVQSERMKKVEIEA"
     gene            complement(1001..>2103)
                     /locus_tag="PK849_13245"
     CDS             complement(1001..>2103)
                     /locus_tag="PK849_13245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE67139.1"
                     /translation="AFLAVFMLYPSVLRDQGFSLGLSGWLMSVFNLCSTLGRPLGARA
                     TERLGIRTTLLWTSGLVAVFTMPLAFTEHPLLLLFLRGTGGVFWGISMVAISSYQGLS
                     IPAARRGSAYAWIAVAYVFPQVTLFPLADWFASSRMLTAYFLLAAAVEAAIVLVAAPL
                     PPLRRAESSQEEPKEWGRWSELPSLPGFRAVLATMFLFAFVNASTLQYLPAFLSMRGY
                     SPSVFIVPNAMTAIALRLVAFRIMDRIDRRRVIGSIIAAMGFILAFLPFAPGRGWYVA
                     GGLFYGVVMGMCFPIVLALMPDLFPERLRPKGISLCLFAIDLGFILSPFFLGYGGQIF
                     GLGRTLSVTGVLGLLGGALLHFAWNGKKRAEP"
BASE COUNT          510 a          546 c          673 g          374 t
ORIGIN      
        1 gccgaagctg taccgagcct tctgtacggc gacggagatg caggggggct ccgagcagca
       61 gacgcctccc aacgcggcga tcatgcctcc gggttttcct tcttcgtcgt acacgccgac
      121 gaggaacgcg ggggccggat acacgggaag aggtttgttg actgctacct tcatgagatc
      181 agctcctttg caaagagtgc gcgcgatgct gcgcgcatcg agtgtattgt aacgcacatc
      241 gcgaagaaac tcccggaggg tcttctctta aaatgtgagg aattcgtttt ttcgatgtga
      301 aaacaaaaga tagaaggtca tttcctatgg tatttatatg tatttacgcc tcaatacggt
      361 atttcaggat tgtctgacca atgctgacgt atataataag gtcaaaggtc agtaaagatc
      421 aaaatcaata aagatcaaac ggaggtgttg taaatgacac ggagatttct ggccatcagg
      481 ccgaacgacg gcaatatgcc cctgaatgtt ttcccttcgg tcgacagaat gatgcgtgac
      541 atgatgcggg ttttcggcga tttttcatcc gacgtggggt atcctgcgga tgcggagaag
      601 atgctcgcga ttcccagggg cgacttctac aggaaggacg gaaagatttt cgccgagttc
      661 gagattccgg gcatcgatcc ttccaaggtg gagttgaacg tctacgagga caggctcgaa
      721 ctccgggcgg agaaatccga cgagaaaacc ttggagcaga acaatgttct ccgctccgaa
      781 cgctacttcg gcacggtgtc acgctccatt cagttcccgg tggaggtcga tccggacagc
      841 gcgaaggcga gctttaagaa cggcgtgttg acagtcgagg tcgcggagcg ggtgcagagc
      901 gagagaatga agaaggtcga aatcgaggcg taagggagcg tctgtttcgc gaaaagcagg
      961 aaagagcgct tgaactggcg gagtcttgga ggaagtatcc tcagggctcc gccctctttt
     1021 tgccgttcca ggcgaagtgc agcagggcgc ctccgagcaa accaagcacg ccggtgactg
     1081 aaagcgtcct cccgaggccg aaaatctgtc cgccgtaccc caggaagaag ggggagagaa
     1141 tgaagccgag gtcgatggcg aaaaggcaga gagagattcc tttcgggcgc aggcgttcgg
     1201 ggaagagatc gggcatcaac gcgagcacta tggggaagca catccccatg acgacgccgt
     1261 agaacaaccc cccggcgacg taccagccgc gtcccggcgc gaaggggagg aaggccaata
     1321 tgaatcccat tgcggcgatg atgcttccga tcacccgtct gcgatcaatc cggtccataa
     1381 ttcggaaggc cacgaggcgc agggcgatcg cggtcatcgc gttaggaacg atgaagacgc
     1441 tcggcgagta ccctctcatg gagaggaagg ccggaaggta ctggagtgtg gaggcgttga
     1501 cgaaggcaaa gaggaacatg gtggcgagca ccgcccggaa tccggggagc gagggaagtt
     1561 cgctccatct cccccactct ttcggctcct cttgcgaact ctccgctcgt cgaagcggag
     1621 gaagcggagc cgccaccagg acgatggcgg cttcgaccgc cgcggcgagc aaaaagtagg
     1681 ctgtgagcat gcgggaggaa gcaaaccagt ccgccagagg gaaaagagtg acctgaggaa
     1741 agacatacgc cacggcaatc catgcgtacg cgctccctct ccgcgctgcg gggatgctga
     1801 gcccttggta tgaggagata gcaaccatag agatccccca gaagacgccg cctgttcccc
     1861 ggaggaagag aagcagcagc gggtgttccg tgaaggcgag cggcattgtg aagacggcga
     1921 cgagcccgga ggtccagagc agcgtggtgc ggatcccgag ccgttccgtc gctcgcgctc
     1981 ccagaggcct gcccagcgtc gagcagaggt tgaacacgct catcagccat cccgagaggc
     2041 cgagagagaa tccctgatcg cggagcacgg aggggtagag catgaacacg gccaggaagg
     2101 cct
//
LOCUS       DAOPKU010000536         2098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_61775_length_2098_cov_5.556045, whole genome shotgun sequence.
ACCESSION   DAOPKU010000536 DAOPKU010000000
VERSION     DAOPKU010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61775_length_2098_cov_5.556045"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PK849_13250"
     CDS             complement(<1..592)
                     /locus_tag="PK849_13250"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPE67140.1"
                     /translation="MGDQDKGYKQLFSNKEMVRDLLVRFVDPAIVQELDLDFRTMVRE
                     HSDFVTDDLRERIDDVIWRVNFRGKTLYLCILIEFQSSEDRWMAVRLLTYLGLLYDDI
                     VRRRLLSDGMLPPILPVVVHTGSSRWKTPCSLEALASPVSPALARYAPSLRYLLLDAA
                     AYTDEELADPENLAEFLFRMEKSRMPMELVEQVRTLG"
     gene            757..1947
                     /locus_tag="PK849_13255"
     CDS             757..1947
                     /locus_tag="PK849_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67141.1"
                     /translation="MERTTAMVISHLEWFSLLRPLEEDVAQSYTLPVEQDAQHASTGV
                     AATLRRRGPNAIARASKEFAETKETLAVSGAQNARTSETLEIKERKNIDAHWKSIIKR
                     FFVRVLRRILPELADDLDTARKVVFLDKELAELTVYIDGPEQRTDVLARVPTKSGRDV
                     WLILHVEIQGPEGGDLPERMFYYNSSLRVTYLKKKSDISDAISFAILTAKRPCGEDEL
                     YLRESYRNRLAYEYPSLKLWELDSVELEASMNPFDWALYAGKCALESGRNDRLKVDYL
                     KKLSEKLDSKGWSHEEKLALFRFMDLLLRPKSPEMRKEFDVYVDRRKKEGKIVYLSMI
                     EENAEKRGEKRGEKRGEKRGEKEKALAVASRMLDAGEPVEKILRYAEISPKELDDLIK
                     TREN"
BASE COUNT          469 a          511 c          706 g          412 t
ORIGIN      
        1 ttcccagcgt gcgcacctgc tccacgagct ccatgggcat ccggctcttc tccatccgga
       61 aaagaaactc cgcgagattt tccgggtcgg cgagttcctc gtccgtgtac gccgcggcgt
      121 ccagcagcag atagcggagc gacggagcgt agcgcgcgag ggcgggggag acggggctcg
      181 ccagagcctc cagagagcag ggtgtcttcc agcggctgct tcctgtgtgg acgacgacgg
      241 ggagaatcgg cggaagcatg ccgtccgaga ggaggcgacg cctgacgatg tcgtcgtaga
      301 ggagtcctag gtaggtcagc aggcggacgg ccatccatct gtcctcggag gactgaaatt
      361 cgatgagtat gcacaaatag agcgtcttcc ccctgaagtt gacccgccat atcacgtcgt
      421 cgatccgctc ccggaggtcg tcggtgacga agtcggagtg ctcgcgcacc atggtgcgga
      481 agtcgaggtc cagctcctgc acgatggcgg gatcgacgaa gcggacgaga aggtcgcgga
      541 ccatctcttt attcgaaaag agctgcttgt atcctttgtc ttgatctccc atgtccgaat
      601 tatagatgcg aaggaagggt ttggcaaggc gcgatggagc gggatccggg gatcgggtcg
      661 gccttcctgt acgcagaggc gcgaacgcgc gtctgcacgt gtacgacccg gtggcggtgg
      721 agaatgctcg gcgtggagcg gcgcggaggg tagcgtgtgg agagaactac cgctatggtg
      781 atctcacatc tcgaatggtt ttctctactt cgtccacttg aagaagatgt cgctcaaagc
      841 tatactcttc ctgtggaaca agacgcacaa catgcttcga cgggtgttgc tgcgaccctg
      901 agacgaaggg ggccgaacgc catagcacgc gcttcgaagg agttcgcgga aacgaaggag
      961 acactcgctg tttccggtgc acaaaacgcg agaacgagcg aaacgctcga gataaaggaa
     1021 cggaagaata tcgacgcgca ctggaagtcg atcatcaaga ggttcttcgt tcgagttctt
     1081 cgaagaattc ttccggagct ggccgacgat ttggataccg cccggaaggt ggtcttcctg
     1141 gacaaggaac ttgcggaact gacggtctac atcgatggtc ccgagcagcg caccgatgtg
     1201 ctcgcccgtg ttccgacgaa gtccggacgc gacgtttggc tgattctcca cgtggagata
     1261 cagggaccgg agggaggcga cctgccggag cggatgttct actacaactc ctctctgcgg
     1321 gtgacgtacc tcaagaagaa gagcgacatt tcggacgcga tctcttttgc cattctgacg
     1381 gcgaagcgcc cctgcggcga ggatgagctg taccttcggg agagctaccg gaaccgttta
     1441 gcgtacgagt acccgtcgct gaagctgtgg gagcttgatt ccgtggagct tgaggcaagt
     1501 atgaatccgt tcgactgggc tttgtacgcc gggaagtgcg cgcttgagag cggccggaac
     1561 gacagactga aggtggacta cctgaagaag ctgagtgaga aactggattc caaggggtgg
     1621 tcgcacgagg agaagctggc gctcttccgc ttcatggacc tgctgctccg tccgaagagt
     1681 ccggagatga gaaaagaatt cgatgtgtat gttgaccgta ggaagaagga ggggaaaatc
     1741 gtgtatttga gtatgatcga ggagaatgcg gagaagagag gggagaaaag aggggagaag
     1801 agaggcgaga agagaggcga gaaggagaag gctcttgcgg ttgcctccag gatgttggat
     1861 gccggggagc ctgtggagaa gattcttcgt tatgcggaga tttcccccaa ggagctcgat
     1921 gacctgatca agacgcggga aaattaaata cgccgctgat cttcgacggg gtttgagtac
     1981 gcgggcatcg aaatgtgtcg aggttgctaa aggtcgacgc ttctcccggg atcttaccgg
     2041 ttggaaggca ggcggctgaa gaggatttcg ttcgcacggt ggtaggactc gaaggtgc
//
LOCUS       DAOPKU010000537         2095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_61918_length_2095_cov_3.561765, whole genome shotgun sequence.
ACCESSION   DAOPKU010000537 DAOPKU010000000
VERSION     DAOPKU010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61918_length_2095_cov_3.561765"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PK849_13260"
     CDS             <1..247
                     /locus_tag="PK849_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPE67142.1"
                     /translation="CAEMAKVLAQFQEKHAAIIEVQIVNVREYPDVARLYQVRYVPTL
                     VFLDEQGTMLEKRVGYMPLEALEKEWRDRGYDLDSKE"
     gene            256..957
                     /locus_tag="PK849_13265"
     CDS             256..957
                     /locus_tag="PK849_13265"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPE67143.1"
                     /translation="MGEIYSSLYSVFHGTSLWAYAAVFAWGILGMILSPCSIATIPLV
                     VGYVGNTDAPDFKTAFKISLVFSLGVFVNLAFLGGLLASAGLFLGGIDQYTNYIVAGV
                     FVLFGLHLLGLISIPWFRGSSGPTTKHTGLKGALALGTLSGLALGPCSFAYSAPVILL
                     AMQTASTDIVRALLLVMAYGAGHSTVIIAAGSATDVFSRFVSKGGQGSVWLGRISGLL
                     LLAAAAYLVYNAPTV"
     gene            991..1530
                     /locus_tag="PK849_13270"
     CDS             991..1530
                     /locus_tag="PK849_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPE67144.1"
                     /translation="MDFVDLVRKRYSLRKYSPFPVAVPVIEKCLEAARLAPSACNSQP
                     WSFLVAATPEKVKPLAEAAFSGVYSMNSFAAGAPVLIVAITERSRYSAALGGMFRGVQ
                     FSLMDVAIACEHISLQAAELGLGTCMFGWLNEKAVKKALDLPSSAKIDLMLSMGYPED
                     EEFLPPKRRKSLEEIRRFL"
     gene            1624..>2095
                     /locus_tag="PK849_13275"
     CDS             1624..>2095
                     /locus_tag="PK849_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPE67145.1"
                     /translation="MIALYVILGIAALVLLWGIGIYNGLIKKVNLKNEAWSGIDVQLK
                     RRFDLIPNLIETVKGYAGHEKEVFLKVTEARSAIGQSKSVAERAESENMLSGALKSLF
                     AVAEGYPELKANTNFLQLQEQLSSLENDIQMSRRYYNGAARDYNIAIATFPAVIV"
BASE COUNT          426 a          594 c          611 g          464 t
ORIGIN      
        1 gtgcgccgag atggccaaag ttttggccca attccaggag aagcacgccg ctattatcga
       61 ggtccagata gtgaatgtgc gggaataccc ggatgttgcg cgtctctatc aggttcggta
      121 cgtacctact cttgtcttcc tcgacgaaca aggaaccatg cttgaaaaaa gagttgggta
      181 catgccgctt gaagctctcg aaaaagagtg gcgggaccgc gggtacgacc tggattcaaa
      241 ggagtaacgc ggtttatggg cgaaatatat tcctctctct actctgtatt tcacggaacg
      301 agtctttggg cgtacgcggc ggtgttcgca tggggaattt tggggatgat cctcagcccg
      361 tgcagcatcg ccacgatacc gctcgtcgtc gggtacgtag ggaacaccga cgcccccgac
      421 ttcaagacgg cgttcaaaat atcgctcgtt ttctcgctgg gcgtttttgt caaccttgcg
      481 ttccttggag gcttgcttgc ctcggcaggt ctctttctcg gcggtatcga ccaatatacg
      541 aactacattg tcgcgggcgt tttcgttctg ttcggattgc atctgctcgg cctgatctcc
      601 attccgtggt ttcgcgggtc atccgggccg acgaccaagc atacgggtct gaagggagcg
      661 ctcgcgctcg gcacgctctc ggggctcgct cttgggccgt gcagctttgc ctactcggcg
      721 cccgtcatcc tgctcgcgat gcagacggcc tccacggata tcgttcgcgc gctgctcctt
      781 gtgatggcct acggcgcggg acacagtacg gtgatcatcg cggccggaag cgccacagac
      841 gtcttctcca gatttgtctc gaagggcgga cagggaagcg tctggctggg cagaatcagc
      901 gggcttctcc tcctggcggc tgcggcgtac ctggtgtaca atgctcctac ggtgtaaccg
      961 cctattccga atcccaagga gggatcgcca gtggacttcg tcgatcttgt ccggaaacgc
     1021 tacagcctga gaaagtacag cccgttcccc gtcgccgtac cggtgattga aaagtgtctc
     1081 gaagcggccc gccttgctcc gtcggcgtgc aactcgcagc catggagttt tcttgtcgcc
     1141 gcaacgcccg aaaaggtgaa accgcttgcc gaggcggcgt tcagcggcgt ctattccatg
     1201 aactcgttcg cagccggcgc gccggtgctc atcgtcgcga tcaccgaacg ctctcggtac
     1261 agcgcggctc tcggcggcat gttcagggga gtgcagttca gcttgatgga cgtggcgatc
     1321 gcatgcgagc acatctcctt gcaggcggcg gaactcgggc tcgggacatg catgttcggc
     1381 tggctgaacg agaaagccgt caagaaggcg ctcgatcttc cgtcctccgc caagatcgac
     1441 ctgatgctgt ccatggggta ccccgaggac gaggagttcc ttcccccgaa acgacgaaaa
     1501 agtctcgaag agatcaggcg ttttttgtag cgcggccggt acgtatattt tcctgtgcgc
     1561 ttccttccgg gaatggtaga atgatggcaa gaaatccatt gattacgcca aggagggaaa
     1621 aaaatgatag cactgtatgt gatactcggt attgcggcgc tcgttctgct ttggggaatc
     1681 ggcatttaca acggtctcat caaaaaggtg aacctgaaaa atgaagcgtg gagcggcatc
     1741 gacgtgcagc tcaaacgacg cttcgacctc atccccaacc tcattgaaac ggtcaagggg
     1801 tatgcggggc acgagaaaga ggtttttctg aaggttacgg aagcccgctc cgctatcggc
     1861 cagtcgaagt cggtcgccga acgggcggaa tccgagaaca tgctctccgg cgcgctgaaa
     1921 tccctcttcg cggtggctga aggctacccc gaactgaagg ccaacacgaa cttcctccag
     1981 cttcaggaac agctctcctc cttggagaac gatatacaaa tgtcccgccg ttactacaac
     2041 ggcgcggcca gagattacaa cattgccatc gcgaccttcc ccgcagtcat cgtgg
//
LOCUS       DAOPKU010000538         2092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_62044_length_2092_cov_3.729013, whole genome shotgun sequence.
ACCESSION   DAOPKU010000538 DAOPKU010000000
VERSION     DAOPKU010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62044_length_2092_cov_3.729013"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1231)
                     /locus_tag="PK849_13280"
     CDS             complement(5..1231)
                     /locus_tag="PK849_13280"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE67146.1"
                     /translation="MKITIHRGSREIGGVCIQVQSGDRSILLDAGSPLGESLSTVDLN
                     ALDFSDIVISHPHQDHFGLIETLSSKKTVYMGPIARKLIEATRVFLQQPPLICSFGEL
                     QSREWVDFGSDIRIMPYLMDHSCPDAFGLLIEAEGKRVYYSGDFRASGPRKKAFGYFL
                     AHPPRDVDVLLMEGTMMGRGNARFPNEKAVENGMIEVLKQLGDRACFLISSGRHVDRL
                     CAAYSACLETGRDFVVDMYTAHVLRTVSDEFPSVPDINTSSRLKVLANGCTAGAHYVR
                     IKENRKFFGDFKDDLFRPETRVSKEQIAANPSRYFLKISNFSDMLRRLSPCGVIYSMW
                     SGYLEEPRFKRIKEDPRVEFHEIHTSGHAVVDDLRKMVDAIRPKRIVPVHTEHPEAYE
                     MFGDVLLLPDGQPLDL"
     gene            1344..1709
                     /locus_tag="PK849_13285"
     CDS             1344..1709
                     /locus_tag="PK849_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67147.1"
                     /translation="MIYKKEITIEYADLPDRFSQRALTRAEPPAKNIFMIYFSKRSPH
                     ECGLKTPSRRISRNGKGRTKMPAVKEKKNVPLWAREPDPLEPPLTPEEEEAFETGRKN
                     VQRKDCLTLEEFIEQVSLL"
     gene            1724..1960
                     /locus_tag="PK849_13290"
     CDS             1724..1960
                     /locus_tag="PK849_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67148.1"
                     /translation="MDKAVLRRLARIPEPDRGRLTAAIVDLRSDPEAKDILPLTGRTG
                     YRMRVGRWRVLLDIDFKSEKIFVYDLGPRGDVYK"
BASE COUNT          473 a          538 c          630 g          451 t
ORIGIN      
        1 tgttttatag gtccagcggc tgtccgtcgg gaagaagcag gacgtcgccg aacatttcgt
       61 acgcttcggg gtgttccgta tgcacgggta cgatgcgctt cggacggatg gcgtccacca
      121 tttttcgcag atcgtccacg acagcgtgtc cgctggtgtg aatttcatgg aattcaaccc
      181 tcgggtcttc cttgatgcgt ttgaatctcg gctcctccag atatccggac cacatggagt
      241 agatcacgcc gcacggagag agacgccgca gcatgtcgga gaaattgctg atcttgagga
      301 agtagcggga tggattcgct gcgatctgtt ctttgctcac gcgtgtttcc ggacgaaaga
      361 ggtcgtcctt gaaatcgccg aagaacttcc ggttctcctt gattctgacg tagtgcgctc
      421 cggcggtgca gccattcgcc agaaccttga gacgcgacga agtgttgatg tcgggaaccg
      481 acgggaactc gtccgagact gtgcgcagaa catgcgcggt gtacatgtcg acgacgaagt
      541 cccgcccggt ttccaagcat gcgctataag cggcgcagag gcggtcgaca tgtctgccgg
      601 aagagatcag gaagcaggcg cggtcgccga gttgtttcag cacctcgatc attccgttct
      661 cgacggcttt ctcgttcggg aaacgggcgt tgccgcgccc catcatcgtc ccttccatga
      721 gcagcacatc cacgtctcgt ggaggatggg cgagaaaata gccgaacgct ttctttcgcg
      781 gaccgctcgc gcgaaagtcg ccgctgtagt agacgcgctt cccttcggcc tcgatcagaa
      841 ggccgaaagc atcgggacag gagtggtcca tcaggtaggg catgattctg atgtccgatc
      901 cgaaatcgac ccactcccgg ctctgtagtt cgccgaagga gcagatcaaa ggaggctgct
      961 gcagaaatac gcgcgtcgcc tcgatgagct tccgcgctat cggccccata tacaccgtct
     1021 ttttggagga gagcgtttcg atcaggccga agtggtcctg atgagggtgg ctgatgacga
     1081 tatcgctgaa gtcgagcgcg ttcagatcga cggtggaaag agactctccg agaggggaac
     1141 cggcatcgag aagaatggaa cgatcgccgg actgaacctg tatgcagacg ccgccgatct
     1201 cccggctccc tctgtgaatg gtgattttca tgtcgcatcc cccttgcaaa aagatatgag
     1261 acgcatgtcc tgtattcgaa ccgaggaagt gttgcttttg atatcatagc atggaggggt
     1321 gttcgattca gggcttttag agagtgatct acaagaaaga aattaccata gagtatgcag
     1381 atttacctga tcgtttctcg caacgagcgc ttacacgtgc ggaaccgccg gcgaagaata
     1441 tttttatgat atacttttca aagaggtctc cgcatgaatg cgggctgaaa accccctcaa
     1501 gaagaatatc tcgaaatgga aaggggagga cgaaaatgcc ggctgtgaag gaaaaaaaga
     1561 atgtcccttt gtgggcacgg gagcccgacc cgttagagcc gcccctgact ccggaagaag
     1621 aggaagcctt cgaaacgggc aggaagaatg tgcaacgaaa agactgtctg actctggaag
     1681 aattcatcga acaggtctcc ttgttgtgag ctgggaggtg actgtggata aagcagtcct
     1741 ccgtcgcctc gctcgcattc ccgaacccga tcgcggacgt ttgacggcgg cgatcgtcga
     1801 tttgcggagc gacccggagg cgaaggacat actcccttta acggggcgca ccgggtatcg
     1861 tatgcgcgtt ggccgttgga gagtgctcct tgatatagat tttaaaagtg agaagatctt
     1921 cgtgtatgac ctcggccctc gcggagacgt gtacaagtaa acgcagtccc gcacgaaaca
     1981 ctaagccccg gcgcgcatct gagtgcgcgc cggggctcgg tgtttgtcga aaactcccta
     2041 cagcaccgag gagatcaggt ccttgtgcgg ggaggggatg acggcggagc tg
//
LOCUS       DAOPKU010000539         2091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_62099_length_2091_cov_3.060413, whole genome shotgun sequence.
ACCESSION   DAOPKU010000539 DAOPKU010000000
VERSION     DAOPKU010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62099_length_2091_cov_3.060413"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PK849_13295"
     CDS             complement(<1..835)
                     /locus_tag="PK849_13295"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPE67149.1"
                     /translation="MRAVVKYKREDGNTEVRDVPVPEIEASDVLVKVAYIGICGSDPH
                     MYHNQVSYPMAIPLILGHEFSGTIEKVGTDVKKWKVGDRVTADTHARFCGKCVLCKTN
                     NYRFCRERKGYGFGVDGAFAEYVRVPERILHAVPDSVTLRDASCTEPVCVAYNVVIAK
                     TGVKAGDCVAVLGPGPIGLLCAHMAKLAGASHIAVFGAPGDEQRLVIAKQYGATETHL
                     LDGSSDPKSVAAKFNEGYGFNSVIDAAGPAATLKLSMELVMPTGIINKVAWGPKPVDF
                     SL"
     gene            complement(885..>2091)
                     /locus_tag="PK849_13300"
     CDS             complement(885..>2091)
                     /locus_tag="PK849_13300"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPE67150.1"
                     /translation="GLAIGTGLIASFVGTIFSILVMIFLSPLVYKIALRFGHWELFLL
                     ALFGIMICGTLSAGKDPLKGWIVGFLGFAAAMVGMEQIFAYPRLTFNFLQLKGGIPLI
                     PALIGVFGISEFLAVLQENVAYKIDGKVGKIIPPMKEFIPLIPSAIRSGVIGVVIGAI
                     PGAGEDIGSWLSYDVGKRRSKQGHKFGTGSYEGLVCSEVANNAVIGGAMIPLLTLAVP
                     GSPPSAMFLAAVWLHGVRPGPMLNFENPGFLPLVAAMLILGSFAMLFWGLALAKPMVA
                     ILKIKREILLPIVIPLTVIGAYAGGVRIFDVYLMLAFGVLGYILRQMDYPLAPLVLGL
                     ILGPLADTSFRRALQQSRGAIMPLLERPIGLLLLLCVAWLIWTGIKRTRQYYAEQTAR
                     EDGEELSAE"
BASE COUNT          388 a          642 c          684 g          377 t
ORIGIN      
        1 cgaggctgaa atccacgggt ttcggccccc acgctacctt gttgatgatt cctgtgggca
       61 taacgagctc catcgagagc ttcagcgtcg cggcgggtcc tgccgcgtcg atgacggagt
      121 tgaagccgta cccctcgttg aacttcgcgg ccacgctctt cgggtccgag gatccgtcga
      181 ggaggtgcgt ctcggtcgcg ccgtactgtt tcgcaatgac aaggcgctgt tcgtcgcccg
      241 gcgcgccgaa gacggcgatg tggctcgccc cggcgagctt tgccatgtga gcgcagagta
      301 gccctatcgg tccgggaccg aggacggcga cgcagtcgcc cgccttgacg cccgtcttcg
      361 cgatcaccac gttgtacgcc acgcagactg gctccgtgca tgaggcgtcc cgcagcgtaa
      421 ccgaatcggg aaccgcgtgc agaatacgct ccggcacgcg gacatactcc gcgaacgcgc
      481 cgtcgacgcc gaaaccgtac cctttgcgct cgcggcagaa gcggtagttg ttcgtcttgc
      541 agaggacgca cttgccgcag aagcgggcgt gcgtatccgc ggtcacccgg tcgccgacct
      601 tccatttttt cacgtcggtc ccgacctttt cgatggtccc cgagaactcg tgtccgagga
      661 tgagggggat cgccatgggg taggagacct ggttgtggta catgtgcggg tcgcttccgc
      721 agatgccgat gtacgcgacc tttacgagca cgtccgacgc ttcgatctcc ggtaccggga
      781 cgtcccgaac ttccgtgttg ccgtcttctc tcttgtattt caccactgcg cgcatagtcc
      841 gttctccctc ctcgccgccg ggaacatcgg cggcgtttcg ttgtctattc cgcggagagc
      901 tcctctccgt cctctcttgc cgtctgttcg gcatagtact gtcgtgtgcg ttttatcccc
      961 gtccagatga gccaggcgac acacagcagg agcaacagcc cgatggggcg ttcgagcagc
     1021 ggcatgattg cgccgcggct ctgctggagc gccctgcgga agctggtgtc ggccaaggga
     1081 ccgaggatga gcccaaggac gagcggcgcc agcgggtagt ccatctgccg caggatgtac
     1141 cccagaacgc cgaaggcaag catcaggtag acgtcgaaga tgcggacgcc tcccgcgtag
     1201 gccccgatga cggtcagggg gatgacgatg gggagcagga tctcccgctt gatcttcaaa
     1261 atggccacca tcggtttggc gagcgcgagc ccccagaaga gcatggcgaa cgagccgagg
     1321 ataagcatgg ccgccacgag ggggagaaag ccggggtttt cgaagttgag catcgggccg
     1381 gggcggacgc cgtgaagcca gacggcagca aggaacattg ccgagggggg gcttcccggt
     1441 acggcgaggg tgagcagcgg gatcatcgcg ccgccgatga cggcgttgtt cgccacttcg
     1501 gaacagacca gcccttcgta gctccccgtg ccgaacttgt gcccctgttt ggagcgcctc
     1561 ttgcccacgt cgtaggagag ccagctcccg atatcctcgc cggctcccgg aatggcgccg
     1621 atgacgacgc cgatgacgcc cgagcgaatt gcggacggga tgagcggaat gaactccttc
     1681 atcggcggaa tgatcttccc gacctttccg tcgatcttgt acgctacgtt ctcctgaagc
     1741 acggcgagaa actccgaaat gccgaagacg ccgatgagcg ccggaatgag cgggatccct
     1801 cctttgagct gaaggaaatt gaaggtcagg cgggggtagg cgaagatctg ctccatcccc
     1861 accatggcgg cggcgaagcc gaggaaaccg acgatccacc ctttgagcgg gtccttcccg
     1921 gcggagagcg tgccgcagat catgatgccg aagagggcca ggaggaagag ctcccagtgc
     1981 ccgaagcgga gcgcgatctt gtacacgagc ggcgagagga agatcatcac cagaatgctg
     2041 aagatggtgc cgacgaagga ggcgatcagg ccggtgccga tggcgagccc t
//
LOCUS       DAOPKU010000540         2084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_62409_length_2084_cov_3.390833, whole genome shotgun sequence.
ACCESSION   DAOPKU010000540 DAOPKU010000000
VERSION     DAOPKU010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62409_length_2084_cov_3.390833"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK849_13305"
     CDS             <1..315
                     /locus_tag="PK849_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HPE67151.1"
                     /translation="VDDVVLFKPLQQEEIRRIVRLLASSLEERLKDRNISLEITDAGV
                     DLIAREGYDAVYGARPLKRFMVRRIETPIARMIISGEAAEGSRIVVDERGGALSLSAS
                     CL"
     gene            379..711
                     /locus_tag="PK849_13310"
     CDS             379..711
                     /locus_tag="PK849_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67152.1"
                     /translation="MNVNSVGTTRPLPPLPVEDIVEEKVEETKEEGLKKVEKAKRAVE
                     TERLEIMDRHHETVKKSAETYDKIVKKRLMEKEIEERRLQQRELLAERNEEAERRRDE
                     MRKAAEKD"
     gene            727..1641
                     /locus_tag="PK849_13315"
     CDS             727..1641
                     /locus_tag="PK849_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPE67153.1"
                     /translation="MKKRLPEALRASSVVFVMLFFLLFAGQPLHAASVEENGTYADFD
                     GLRVHYVDEGPRERPALVFIHGWSCDVSFWRFQTEDLRKKFRVIAIDLPGFGLSDKPA
                     DRAYTLDLFAGAARAVVEAAGVEKPVLIGHSMGYPVTTKYLALSPGEVRAIVNVDGAY
                     FRSPSNPRTIEEMKEMNAFLEGLLGPERPQFIGFFIESTFYGRTPQPLRTEIMEKMAS
                     ADEYAANSSMKEFIVPENWEERSYDVPALAVYSAHEYVPPDHEAYLRTVFPNLTYEEW
                     PDTGHFLMMEAPERFNAALVKFLEGLPE"
     gene            1772..>2084
                     /locus_tag="PK849_13320"
     CDS             1772..>2084
                     /locus_tag="PK849_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67154.1"
                     /translation="MENKTEKLILLPCFDSPEAAQACRRDLKIPREKAITLGTSAMEA
                     CDRGYYFDEQGAKVDWGQLVMGACFDARTIPPEFSLPTPTRTPFPETTVQTCIVHLIR
                     HS"
BASE COUNT          443 a          561 c          668 g          412 t
ORIGIN      
        1 gtggacgacg tggtgctctt caagccgctt cagcaagagg agatacggcg tatcgtgcgc
       61 ctgctcgcct cgtccctgga ggaacgtctg aaggaccgga atatctcgtt ggagatcacc
      121 gacgcgggag tggaccttat cgcccgcgag gggtatgatg cggtgtacgg ggcgcggccg
      181 ctgaagcggt tcatggtgcg ccggatagaa acgcctatcg cccggatgat catctccggc
      241 gaggccgccg aggggtcgcg gatcgtcgtg gacgaacggg gcggagctct ttcgctctcg
      301 gcctcctgtt tataacgcgc ccgccggatg ccgatacata agcagctttt tttgaaacac
      361 tgaaaggggg ggatgccgat gaacgtcaat tccgttggta cgacgcggcc tcttccccct
      421 cttcccgtcg aggacattgt cgaagaaaag gtcgaagaga cgaaggaaga ggggctgaag
      481 aaggtcgaga aggcgaagcg cgcagtcgag acggagcgcc ttgagatcat ggacaggcat
      541 cacgagacgg taaaaaaatc cgcggagacg tacgacaaga tcgtaaaaaa aaggttgatg
      601 gagaaggaga tcgaggagcg tcgccttcaa cagcgcgagt tgttggccga acggaacgaa
      661 gaggccgagc ggcggcgcga cgagatgcgg aaagccgcgg aaaaagacta aaacggaggg
      721 gtattggtga aaaaacgtct tcccgaagcg ctgagagcgt cttcggtagt ttttgtaatg
      781 ttgttttttt tgttgtttgc aggacagccg ctgcatgcgg cgtccgtcga agagaacggg
      841 acgtacgccg atttcgacgg actcagggtg cattatgtgg atgaagggcc cagggagcgc
      901 cccgcgctcg tcttcattca cggctggtcg tgcgacgtct ccttctggcg gtttcagacc
      961 gaggatctgc gcaagaaatt tcgcgtgatc gcgatcgacc tgccgggatt cgggctgagc
     1021 gacaagcccg ccgacagggc gtacacgctc gatctctttg cgggcgcggc ccgggcggtc
     1081 gtcgaggcgg ccggagttga gaaacccgtc ctgatagggc acagcatggg gtaccccgtg
     1141 acgacgaagt acctcgcgct ctctccgggg gaggtccgcg cgattgtcaa cgtggacggc
     1201 gcctacttcc gctctccgtc gaacccgagg acgatcgagg aaatgaaaga gatgaacgcg
     1261 ttccttgagg ggctgcttgg ccccgagcgt ccgcaattca tcggcttctt catcgaatcc
     1321 acgttctacg gcagaactcc gcagccgttg cgtaccgaga taatggagaa gatggcatcc
     1381 gccgatgaat atgcggcgaa cagctcgatg aaagagttca tcgtaccgga gaactgggaa
     1441 gagcgctcct acgacgttcc tgccctggcg gtctactccg cgcacgaata cgtcccgccc
     1501 gatcacgagg catacctgcg gacggtcttt ccgaatctga cctacgaaga gtggccggat
     1561 acgggccact tcctgatgat ggaggcccct gagcgcttca acgccgcgct ggtgaagttc
     1621 ctggaagggc tgcccgagta gagtgaagga ggaggggaga ttttccctcc tccttcttcg
     1681 cattcccctg gatatgcgtt tggttttagg atgtgaagtt cattagccgt tctgtttgtg
     1741 gttccatgaa agttgatctg cgagggatgc tatggaaaac aaaaccgaaa aactgattct
     1801 gctgccctgt ttcgactccc cggaagcggc gcaggcgtgc cgccgggatc tgaagattcc
     1861 ccgcgagaag gcgatcaccc ttggcacttc tgcgatggag gcgtgcgaca gagggtacta
     1921 ttttgacgag caaggagcga aggtggactg gggccaactc gtcatggggg cgtgcttcga
     1981 cgcacggact atccctcccg aattttccct gcccacgccc acccgcacgc cctttcccga
     2041 gacgacggtc cagacctgta tcgtccatct gatccggcat tcga
//
LOCUS       DAOPKU010000541         2082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_62505_length_2082_cov_3.766157, whole genome shotgun sequence.
ACCESSION   DAOPKU010000541 DAOPKU010000000
VERSION     DAOPKU010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62505_length_2082_cov_3.766157"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PK849_13325"
     CDS             complement(<1..1125)
                     /locus_tag="PK849_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPE67155.1"
                     /translation="MMLSPLFVFSPGRALLALLAFALSSATCVYARRYFEGADERRFF
                     MYGSLAFLLSVWGTLFSAYWITFAVFMEISTAFLFSLIALKERDVALRYFVVQLVGAA
                     FLLAGIGAAYAGHGPALVGPVPKDALPFFVLGLGVKAALPVLHFWLPEVHGRAPAPVS
                     ALLSGMAVKIGAFGLLQALGADGPNFLAPVGAIMALWGVALALAQHDMKRLLAYHTVS
                     QLGYVIAAVGAGSASGWCGALYHMAAHGLFKSMLFLCAGVLEKVYGTRALDRLGGAWK
                     TLPFTCVLYVVAAAAIAGLPGTIGFASKSVVKSALFEHMLVSFALLAANVGTVMSFCK
                     MGWFAFGGSERSTPLSFAGVRSMNVALLPLAGMVVLFGFFP"
     gene            1497..>2082
                     /locus_tag="PK849_13330"
     CDS             1497..>2082
                     /locus_tag="PK849_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HPE67156.1"
                     /translation="MKLRTKIVNALILLTFAVLVTAAPVMAADTVVIVDFSWDSVQMH
                     NRIAGYVIEKAFGKKVDYIFAESLPGMMGIERGDAHLSMEGWVDNLLEVWERMTSSGK
                     AESLGTNFPDAPQGWYVPTYMIKGDKERGIEPVAPDLKSVADLAKYKDLFIDPENPKL
                     GRLYNGPAGWRVTSINEMKMKGYGLEKHFEAFSAG"
BASE COUNT          573 a          591 c          540 g          378 t
ORIGIN      
        1 ggggaagaag ccaaagagca caaccattcc ggcaagaggg agaagcgcaa cgttcattga
       61 acgaacgcca gcaaaactca gcggagtact cctttcggag ccgccgaagg cgaaccagcc
      121 catcttgcag aacgacatca cggtaccgac atttgcggca agcagggcga acgacaccag
      181 catatgctcg aaaagcgcgc ttttcaccac agatttgctc gcgaaaccga tcgttccggg
      241 aagacctgca atagccgcgg cggcaaccac gtagaggacg caggtgaaag gaagcgtttt
      301 ccatgcgcct ccgagacggt ccagtgcacg cgtgccgtac actttctcca gaacgcccgc
      361 gcataaaaag agcatactct tgaaaagccc gtgagccgcc atgtgataga gcgccccgca
      421 ccatccagaa gcacttccgg cgccgaccgc ggcgataacg tagcccaact gactcaccgt
      481 atgataggcc aagaggcgtt tcatatcatg ctgcgcaaga gccagagcaa cgccccaaag
      541 cgccattatc gcgccgacgg gcgccagaaa gttcggaccg tcggctccga gcgcctgaag
      601 caggccgaat gcgcctatct tcaccgccat tccggagagc aacgcgctca ccggcgcggg
      661 ggctcttccg tgaacctccg gaagccagaa atgaagcaca ggaagcgccg ctttcacgcc
      721 taggccgaga acaaaaaacg ggagcgcatc tttcggaacc gggccgacaa gagccggtcc
      781 atgaccggcg tatgcggcgc ctatacccgc cagaagaaaa gccgccccta caagttgcac
      841 aacaaaataa cggagtgcta cgtcacgctc tttcagcgcg atgagcgaaa aaagaaacgc
      901 tgtggagatt tccatgaaga ctgcgaacgt gatccagtac gctgaaaaga gcgttcccca
      961 taccgagagc aaaaaggcaa gagaaccgta catgaagaaa cggcgttcgt ccgccccctc
     1021 gaaatacctc ctggcgtaga cgcaggttgc agagctcaaa gcgaatgcca gcaacgccag
     1081 caacgctctg cccggcgaaa aaacaaaaag cggagaaagc atcatgaggc cagttttcct
     1141 gaaaggaact ccgtacacat gacgacgtct ccgaacctgc tctgaaaggt catcatctac
     1201 gcatctctcc tctttcagcc tttcttgagt tcgtgtcgaa taccccaaca aatcggacga
     1261 aaacagtata acaccatcga aacaacgaac gaatgaagaa gaaccgcata cggagcaact
     1321 ccagccaaaa cagagtcttt tgtatacttt ttggatattt acatgttgac aaatactcaa
     1381 acttgagttt aaatcagata tcagctccga agcgcccgtc aaacttaccg cttttttccg
     1441 attcatcgca tttttctact actcatggtg aatcaaatcc aacaggaggt gtttgcatga
     1501 aactgcgaac aaaaattgtg aatgcgctaa tcttgctcac gtttgccgtc ctcgttacag
     1561 ccgcgccggt tatggctgcc gatactgtcg tcatcgtcga cttcagctgg gacagcgtcc
     1621 aaatgcacaa tcgcatcgcc ggttatgtca tcgagaaagc cttcgggaag aaagttgact
     1681 acatctttgc agagtctctt ccggggatga tggggattga acgcggcgac gcgcacctct
     1741 ccatggaggg gtgggtcgac aacctcctcg aagtatggga gcgaatgacg tcgagcggca
     1801 aggcggagag cctggggacg aacttcccag acgccccgca ggggtggtac gttcctacgt
     1861 acatgatcaa aggggacaaa gaacgcggca tcgagccggt tgccccggac ctcaaatcag
     1921 tggccgatct tgccaagtat aaggatctct tcatcgatcc ggaaaatccg aaactcggaa
     1981 gattgtacaa cggtccggcc ggctggcgcg tcacttccat caacgaaatg aagatgaagg
     2041 gctacggtct tgagaagcat ttcgaagcct tttcagccgg gt
//
LOCUS       DAOPKU010000542         2081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_62533_length_2081_cov_4.588845, whole genome shotgun sequence.
ACCESSION   DAOPKU010000542 DAOPKU010000000
VERSION     DAOPKU010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62533_length_2081_cov_4.588845"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..803)
                     /locus_tag="PK849_13335"
     CDS             complement(111..803)
                     /locus_tag="PK849_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE67157.1"
                     /translation="MTYINVLVVEDDPMVAEIHQNFINSVDNFRVAGIVNNGLKALEF
                     IERRPVRLVILDIFLPGLDGIATLEKIRERGENIDVIVVSASRDVSTVAKAIQAGAFD
                     YIVKPFVFERIRSSLLSFREMMGHLKGTTQEIDQQTIDTLFQVRNRKHYDSKLPKGLN
                     PHVLERLETMLSGTNVSLSSAEAAEALEISRITARRYLEFLVSTGKAVMEQEYHEVGR
                     PVNKYTLMKQTP"
     gene            complement(803..>2081)
                     /locus_tag="PK849_13340"
     CDS             complement(803..>2081)
                     /locus_tag="PK849_13340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HPE67158.1"
                     /translation="NLPQKGRITPSRPFFFSFGKGITVMKGQADLALAMMERLSAMKQ
                     SGEISKITGAWLSQYDPPVLSRQEIWNIVGGGLLALFAALLWNLLLKRKVGIMVKERE
                     KILDFIKDGILAVDKGGRITMLNRSAQNLLGLSSDSIGKEADLCIPGLAVGIVLTDGR
                     PVYNLQQNLNGALVSCNKVPILEDSHSSGVIITLRDMSELQAMAEEITGVRTYVETLR
                     VQGHEFMNKLQTISGLIQLQRYDRAIEFIASETDSSLSAQAFLAERIKNAAVCGIIMG
                     KAGRCRELGIAFILDPESFCCDHGESINDRSLVIIIGNLLQNAIEAVVETGVTPDARI
                     EFSIFDESGQILIDVFDNAGIMTEEAASRLFEKGFTTKYKAEPSGFGLFNIKTIVDSL
                     AGNISMDFETGRFTEFTVTLPVTAGALRPEVAI"
BASE COUNT          502 a          523 c          517 g          539 t
ORIGIN      
        1 gaaatctttt cgctggtttg tgccaagccg tgaacaaaaa tgtacaaaac gaagagcgtg
       61 ttcaaaaatt cttatgaaaa agcgtaagaa taccgtatac gagactctcg ctatggggtt
      121 tgcttcatga gcgtatattt attcacagga cgtcccacct cgtgatactc ctgttccatg
      181 acagcttttc ctgttgatac caaaaactcc aggtagcgtc tagcggtgat ccgtgatatt
      241 tccagcgctt ctgccgcttc tgccgaagaa agcgacacat tcgtcccgga gagcattgtt
      301 tccagacgct ctagtacgtg cgggttgagg cctttaggga gtttgctgtc gtagtgcttc
      361 ctgtttcgca cctgaaaaag ggtatcgatc gtctgctggt caatctcttg agttgttccc
      421 ttgagatgtc ccatcatctc gcggaaagag agaagggatg agcggattcg ctcgaaaacg
      481 aagggtttta caatatagtc gaatgccccc gcctgaattg ccttcgcaac tgtagaaaca
      541 tcccttgatg cggaaacgac gatcacgtca atattctcac ctctctcccg tattttttcg
      601 agagtggcta ttccgtccaa accggggagg aaaatatcca gaatgaccag tctgacaggg
      661 cggcgttcta tgaattccag ggccttcaga ccgttattga ctattccggc aacacggaaa
      721 ttatcgacgg agttaatgaa attctgatgg atttcagcga ccatcggatc gtcttccact
      781 acgagaacat tgatgtatgt cactatatgg ctacctccgg ccggagagcg cccgcggtta
      841 caggcaacgt caccgtaaac tcagtaaagc gcccggtttc aaaatccatt gatatgttgc
      901 cggcgagaga atcaactatg gtcttgatat tgaagaggcc gaaaccggag ggctcggctt
      961 tgtatttcgt tgtgaaccct ttttcgaaaa gcctggatgc ggcttcttca gtcatgattc
     1021 cggcgttatc gaagacgtct atcagtatct gtcccgattc gtcgaagatg gaaaactcga
     1081 ttcgcgcatc gggagttact ccggtttcaa cgacggcttc tatcgcattt tgtaaaagat
     1141 ttccaatgat gatcaccaga gaccggtcgt tgatgctctc tccgtggtca cagcaaaagc
     1201 tctcagggtc gagtataaag gctattccca gttcacggca gcgtcccgct ttgcccatga
     1261 tgattccgca gacggcggcg ttctttattc tctcggccag gaatgcctgc gccgagagac
     1321 ttgagtccgt ttcggatgca atgaattcga tggccctgtc gtagcgttgc agctgaatca
     1381 gtccggaaat tgtctgcaac ttgttcatga actcatggcc ctgtaccctc agggtttcta
     1441 cataggtccg gacgccggta atctcttccg ccattgcctg aagctcggac atatcacgaa
     1501 gtgtaatgat aacgccgctg gagtgagaat cctccagtat gggaactttg ttacaagaaa
     1561 cgagcgcccc gttgagattc tgttgaaggt tgtagacagg ccgtccatct gtcagcacaa
     1621 tgccgacggc aagaccggga atacagagat cggcttcttt gccgatagag tcggaggaga
     1681 gaccgaggag attttgtgcg gagcggttga gcatcgtaat tcgtccgccc ttatccacag
     1741 caagaatacc atctttgata aaatcgagaa ttttctcgcg ctctttgacc ataatcccga
     1801 ccttccgctt cagcagtaaa ttccataaga gcgcggcaaa gagcgctaaa aggcctcctc
     1861 cgactatgtt ccatatctcc tgtctgctta aaacgggcgg atcgtactga gagagccacg
     1921 cgcccgttat cttcgagatt tctcccgact gtttcatggc cgaaagcctt tccatcattg
     1981 caagagccag gtcagcctgc cctttcataa ccgtgatccc tttgccgaac gaaaagaaga
     2041 acgggcggga gggggtgatc ctcccctttt ggggaaggtt c
//
LOCUS       DAOPKU010000543         2076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_62722_length_2076_cov_4.213261, whole genome shotgun sequence.
ACCESSION   DAOPKU010000543 DAOPKU010000000
VERSION     DAOPKU010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62722_length_2076_cov_4.213261"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK849_13345"
     CDS             <1..240
                     /locus_tag="PK849_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67159.1"
                     /translation="GSVLAVVLNWVIVVASVALLVAIASLVLTHLRFIAAGKKGFLLS
                     IVLVISFIATLTLGLLLGVDDPNFLKAVGAIIRHA"
     gene            complement(354..1523)
                     /gene="iadA"
                     /locus_tag="PK849_13350"
     CDS             complement(354..1523)
                     /gene="iadA"
                     /locus_tag="PK849_13350"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HPE67160.1"
                     /translation="MLLIRNADLYAPERLGPGDILIAGERVLAVRPAGEIDSTAAASF
                     SDLRVIDAGGCIVAPGIIDRHVHFNGAGGEGGPAHRTPPLQLSSFIKAGVTSAVGMLG
                     TDGVCRSLNELLMKARGLEVEGISTWILTGSYSFPSPTLTGSVQSDLCLIDKVIGLKL
                     AISDHRSAHPSVEEIRRAVSDVRAGGILSGKAGFLCVHMGSEKEGLEPLLRAIEGTDI
                     PIAQFAPTHISRTEALLEQSVEFGRRGGLLDITAAPGKKPLFGMGTSVAVARLLEGGV
                     DPKNITLSSDGNGSMPSFNEKGEMTGMRVGPLDSVVESVAEMLRDGALPREQVFSFVT
                     ANPAAHLKLPRKGRLKPGFDGDLIMLDGAFRLRTVVAKGRVLMDEGEVVVKGTFE"
     gene            complement(1633..>2076)
                     /locus_tag="PK849_13355"
     CDS             complement(1633..>2076)
                     /locus_tag="PK849_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPE67161.1"
                     /translation="VFFAYIFAGQLEYTGTFRRICTSLKEGFIGNSKGRFVLSASLSG
                     ILTGLGTGNSYLSEIVPGTMYKDLADEMNISRRVLSRTLEDSGTVVVPLIPWSAAGIY
                     MSSVLGVSVFEYVPWAFLCWLGFLTAWFYGFTGIAIWPSDKKADA"
BASE COUNT          371 a          636 c          704 g          365 t
ORIGIN      
        1 ggtagcgttc tggcagtggt tctaaactgg gtgatcgtgg tcgcctcagt ggcgctgctg
       61 gtggcaatcg ccagcctggt gcttacgcac ctgcgtttca ttgccgcagg caaaaagggc
      121 tttttgctca gcatcgtcct ggtgattagc tttatagcca cgctgacact tggattgctg
      181 ctcggtgtgg atgacccgaa cttccttaaa gcggttggcg cgatcatccg acatgcctga
      241 attgtacagt aatgccgtgc gactgtcaaa gacacgagtg tacacaaaag gccggaaggg
      301 acgctccata cgcagagttc cttccggcct tccatgctct gccgaagaga gagctactcg
      361 aaggttccct tgacgacgac ctcgccctcg tccatcagca cccttccctt ggcgacgacc
      421 gtccgcaggc ggaaggcgcc gtcgagcatg ataaggtcgc cgtcgaagcc gggcttgagg
      481 cgccccttgc gggggagctt gaggtgcgcg gcggggttcg cggtgacgaa ggagaagacc
      541 tgttcgcgcg ggagcgcgcc gtcgcgcagc atctccgcga cggattcgac gaccgagtcg
      601 agcggaccga cgcgcattcc cgtcatctcc cccttctcgt tgaaggaggg catggagccg
      661 ttcccgtccg agctgagggt gatgttcttc ggatcaacgc ccccttcgag caggcgggcg
      721 acggcgaccg aggtccccat gccgaagagc ggcttctttc cgggcgcggc ggtgatgtcg
      781 aggagaccgc cccggcggcc gaattccacg gactgctcaa gcagcgcttc ggttcgcgag
      841 atgtgcgtgg gggcgaactg cgcgatcggg atatccgtcc cctcgatggc gcgaagcagc
      901 ggttcgagcc cctccttctc gcttcccatg tggacgcaga ggaatcccgc cttgccggag
      961 agaatgcccc cggcgcggac gtccgacact gcgcggcgta tctcctcgac ggaggggtgc
     1021 gcgctccggt ggtcggagat ggcgagcttc aggccgatga ccttgtcgat cagacagagg
     1081 tcgctctgga cgcttccggt aagcgttggc gacggaaaag agtagctccc tgtgagaatc
     1141 cacgtggaga tcccttccac ttccagtccc ctcgccttca tcagcagttc gttcagagag
     1201 cggcaaaccc catcggtgcc cagcatgccg accgcggagg tgacgcccgc cttgatgaac
     1261 gacgaaagct ggagcggcgg cgtcctgtgg gcgggtcccc cctcgccgcc cgcgccgttg
     1321 aagtgaacgt gccggtcgat gatgccgggc gcgacgatgc agcccccggc gtcgatcacc
     1381 cggagatcgc tgaacgaggc ggccgccgtg gaatcgatct cccccgccgg gcggacggcg
     1441 agcaccctct cccccgcgat caggatgtcg ccgggaccga ggcgctcggg agcgtagaga
     1501 tcggcgttgc gtatcaagag cattggtgtg tctcctttcc cgcgtccggc ccgtgggcgg
     1561 cgcgttgcga tgcggagagg agggaggttc cctcgcttgg cgccgaagga aacctccctc
     1621 ccggcagacg cgctacgcgt ccgccttctt gtcggagggc cagatcgcga ttccggtgaa
     1681 gccgtagaac caggcggtca ggaagccgag ccagcagagg aatgcccagg gaacatactc
     1741 gaagacggag acgccgagca ccgaggacat gtagattcca gcggccgacc aggggatcag
     1801 cggaacgacc acggtgccgg agtcctcgag cgtacgggag agcacgcggc gggagatgtt
     1861 catctcgtcg gcgaggtctt tgtacatcgt gcccgggacg atctcgctga ggtaggagtt
     1921 gccggtgccc agtccggtga ggatgccgga gagcgaggcg gagaggacga agcgcccctt
     1981 gctgttcccg atgaagccct ccttcaggga ggtgcagatg cggcggaagg tgcccgtgta
     2041 ctcaagctgc cccgcgaaga tataggcgaa gaagac
//
LOCUS       DAOPKU010000544         2074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_62813_length_2074_cov_4.186231, whole genome shotgun sequence.
ACCESSION   DAOPKU010000544 DAOPKU010000000
VERSION     DAOPKU010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62813_length_2074_cov_4.186231"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PK849_13360"
     CDS             <1..685
                     /locus_tag="PK849_13360"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HPE67162.1"
                     /translation="ITAEKPDPLRASSLPALAAMPPHMEPGILLNVESTLSYASLGKY
                     VSSMDFPEVDIPGGGRVKVKEVSFFGSGDKLVAAADISGKGPVGGAIEGKVYIMGRPA
                     YSREDETIRMEDADFEEQTNSALAKAAAWLARPALLKEMTERMVFPLQELREQTVRSL
                     AELLREQKIAEDVLMEGSVSALSLESLAVGDNGLRLSLSLGGAVSLRYEDESLKRKEK
                     EAKNGGKKN"
     gene            773..1777
                     /locus_tag="PK849_13365"
     CDS             773..1777
                     /locus_tag="PK849_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE67163.1"
                     /translation="MKRIGLFCVFVLLCALFAGAAFASEKVSAYATTEEPLAKELFDA
                     FEKETGIKVDWVRLSSGEAVARLEAEKANPRASIWVGGVGTLHIDAKNKGLTAPYNSR
                     AASSIPVKFHDPERFWIGLYVGPIAFAINTDRAKELNLALPTSWADIVKPEYAKNVRV
                     ANPGTSGTAYNMVTTIIRVFGNDEDKAFDFLKQLDKSIDQYTRSGSAPGKSCAIGEIP
                     VAIGYLHDLIKLKVENAPIEIALPSDGTGFETAAMSLVKDGPDPVNAKKLYDWMLGQT
                     AMDIIAGWYVIPLSSKASPIDAGYSFEKMTLVDQNDVWDAANKARLLERWNKEIGSAR
                     "
BASE COUNT          444 a          664 c          590 g          376 t
ORIGIN      
        1 aatcaccgcg gaaaagccgg acccgctcag ggcatcttcg ctccccgcgc ttgcggcgat
       61 gccgccgcac atggagccgg gcattctgct caacgtcgag agcacgctct cctatgcatc
      121 tctgggcaag tacgtctcct ccatggactt ccccgaagtc gacattcccg gcggggggcg
      181 cgtcaaggtg aaggaggtct ctttcttcgg cagcggcgac aaactcgtcg ccgccgcgga
      241 tatctcggga aaaggccccg tgggaggcgc tatcgaggga aaggtctaca tcatggggcg
      301 gcccgcctat tcacgggagg acgaaacgat ccggatggaa gacgccgact tcgaggaaca
      361 gacgaacagc gcccttgcaa aggccgcggc gtggctcgcc cgccccgcgc ttctcaaaga
      421 gatgaccgaa cgaatggtct tcccgctcca ggagttgagg gaacagaccg tacggtcgct
      481 cgcggaactg ctgcgggaac agaaaattgc ggaggacgtc ctcatggagg gctccgtctc
      541 cgcgctctct cttgaaagcc tcgcggtagg cgataacggg ctccgcctct ccctctccct
      601 cggaggcgcc gtttcgcttc gctacgagga cgagtcattg aaacggaagg aaaaagaggc
      661 gaagaatggc ggtaaaaaga attgacacaa cgcctctcac tccatatact caaaaaggtt
      721 ttataacggt tcctgaaagt gaacctctat tacagcaaag ggagatgaaa gtatgaaacg
      781 aatcggtctg ttctgtgttt ttgttctgct ctgcgcgctc ttcgccggcg cggcgttcgc
      841 ctcagaaaag gtgagcgcct acgccacaac ggaagaaccc ctcgccaaag aacttttcga
      901 cgccttcgag aaggaaaccg gcatcaaggt cgactgggtc cgtctctcct ccggcgaggc
      961 cgttgcacgc ctcgaggccg aaaaggccaa ccccagggcg tccatctggg tcggcggcgt
     1021 gggcacgctc cacatcgacg cgaagaacaa ggggctcacc gcgccgtaca actcccgcgc
     1081 ggcctcctcg atccccgtca agttccatga tcccgagcgt ttctggatcg gcctctacgt
     1141 gggaccaatc gccttcgcca tcaacaccga ccgcgcgaag gagctcaacc tggcgctgcc
     1201 gacctcctgg gccgacatcg tcaagcccga gtacgcgaag aacgtccgcg tcgccaaccc
     1261 cggcacctcg ggcacggcgt acaacatggt gacgacgatc atccgcgtct tcggcaacga
     1321 cgaggacaag gcgttcgact tcctcaagca gctcgacaag agcatcgacc agtacacccg
     1381 ctccggctcg gcccccggca agagctgcgc gatcggcgag atccccgtcg ccatcggcta
     1441 cctgcacgat ctgatcaagc tcaaggtcga gaacgcgccc atcgagatcg cccttccctc
     1501 ggacggcacc ggcttcgaga ctgctgcgat gtcgcttgtg aaggacggtc cggacccagt
     1561 caacgcgaag aagctctacg actggatgct cggccagacc gccatggaca tcatcgccgg
     1621 atggtacgtc atcccgctca gctcgaaagc ctccccgatc gacgccggct actccttcga
     1681 gaagatgacg ctcgtcgacc agaacgacgt ctgggacgcc gcaaacaagg cacgtctgct
     1741 ggaacgctgg aacaaggaga tcggctccgc caggtagcat ccgccgcact gtgaaaatac
     1801 gaaggggctc ccaagggggc tccttcgcct tttcttcaaa cgcaatggaa aggaggagag
     1861 gaacattcct tcgaaaagat ttctctcgtt gtttctgtcc gttcttcttt tcgcaacgct
     1921 acttttctgg atgaacaatg cgcttacagg ctccttccag tcgatcgcgg agaaccacta
     1981 ccgtaaatcg gccgtcgccg cgatggggtc ggtgccttca aacgacgacg ccgtttccgg
     2041 ttggctccgc cgtattgctc cctcttcggg cgat
//
LOCUS       DAOPKU010000545         2072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_62890_length_2072_cov_4.615766, whole genome shotgun sequence.
ACCESSION   DAOPKU010000545 DAOPKU010000000
VERSION     DAOPKU010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62890_length_2072_cov_4.615766"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(679..>2072)
                     /locus_tag="PK849_13370"
     CDS             complement(679..>2072)
                     /locus_tag="PK849_13370"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HPE67164.1"
                     /translation="PGVQPEHLNDDAVGRLLDNLSEYNISVLYERFVLECLAPLLHGT
                     VNLHVDTTNFSVHGEYVPNEGFKWEIAFGHAKDGRTDLKRFVLNMVADGSGIPLFLEL
                     LSGNASDKKSIVVGMEKATNTLAALGESPLFGIADAAFYSAENIARIHGSWISRVPAT
                     LNEAAALLETGEALLPCSDPRYSFLEKTSSYGGVEQRWFLFRSDEMAGKQEKTLEKRL
                     ERKLADAKKDASALSKTEFFCEADARSAADSFFSEHPLVQGSVLVEEIRRKKGGRGRP
                     ASGAEMEIRYRAKIDPALAPDAVEILRSKCGRFILATSAVGETAPGAEEILGLYKEQG
                     AVEKGFRFLKDKSFHAAEIYLKREERIEGLMFLMTIALAVYNLAEKELREVLSASGES
                     IPAPGGKPMKNPTLDRLFHLFARVTVLVGEESGKRTQRVMNLNDTLKRMLALFGKNFE
                     RYYMDGASSNAYG"
BASE COUNT          380 a          615 c          581 g          496 t
ORIGIN      
        1 ccggccgttc tccctgcacc atccgtcgag ttcccggact ctgatccggg gcgcgtcttc
       61 cggcacggga acggtcatcc gtccgtcaga acggactcga gatcgatatg ctcttcggca
      121 agcgttacct tctccctcgt catttcaaga ccctgcttcg tgcaactcct cttccttcat
      181 tctttcggcg gacttaccgt caaggaacat ccgcgcggag tgtttttccc aagatggctt
      241 ttttcagttc agtcgtacac atctcgcctg tgggctatac gtattacaag aacacgcaaa
      301 atgccatcat gaatttcgta gacgacacgc cagtctccaa ctcggattcg atagcgtcgt
      361 tcgatcttga gcaagatgca tccttgagga cgaggttctt gggagagacg gtctatagct
      421 ataagcagtc ttttccttag actcccggtc agaccgtcta aagttttttc cgcttgtcta
      481 tggaagacta tctcaaattg cttattccct ttcgattcca tatcgctgaa aaagaatctc
      541 tatcggaacg ccttccgaat cctcggccaa ggcctcgtcg gcaagacgat tgtccagttc
      601 gtcctccggc ttttgtatgt actcggcatc ctccgccggt acccgatatt ccgcactgac
      661 atttagagat ttttgtattt atccatatgc gttcgaggag gcgccgtcca tgtagtaccg
      721 ttcgaagttt ttcccaaata aagcgagcat ccgcttgagc gtgtcgttca ggttcatcac
      781 gcgctgggtc cgctttccgc tctcctcgcc gaccagtacc gtgacccgcg cgaaaaggtg
      841 aaagagacgg tccagggtgg gattcttcat cggtttccct cccggagcgg gaatgctctc
      901 tccgctcgcg gacagcactt cccggagttc tttctcggca agattgtaga ccgccagagc
      961 gatcgtcatg aggaacatga ggccttcgat gcgctcctcc cgcttcagat aaatctcggc
     1021 ggcgtggaag gatttgtcct tgaggaagcg gaatcccttc tcgacggccc cctgctcctt
     1081 gtacagcccg agtatctcct cggcgccggg ggctgtttct cctaccgcgc tggtggcgag
     1141 tatgaagcga ccgcacttgc ttcgaaggat ctcgacggcg tcgggagcga gcgcgggatc
     1201 gatcttcgct cggtaccgga tctccatctc cgctcccgac gcgggacgtc ctcttccgcc
     1261 tttcttgcgg cggatctcct cgacgagtac gcttccttga accagcggat gctcggagaa
     1321 gaaggaatcg gccgcggagc gggcgtcggc ctcgcagaag aattccgttt tcgacagcgc
     1381 ggatgcgtcc ttcttcgcgt cggccagctt ccgctcgagg cgtttttcga gcgtcttctc
     1441 ctgcttcccg gccatctcgt cggagcggaa gaggaaccat cgctgctcga cgcccccgta
     1501 ggaagaggtc ttctcaagga aggagtagcg ggggtcggag cagggaagaa gggcttcccc
     1561 ggtttcgaga agcgccgcgg cctcgttcag cgtcgcgggt acccggctga tccacgagcc
     1621 gtgtatgcgg gcgatgttct ccgcgctgta gaaagcggcg tcggctatgc cgaacagcgg
     1681 agattcgccg agtgcggcga gcgtgttcgt cgccttctcc atgccgacca cgatgctctt
     1741 tttgtccgag gcgttgccgg agagcagttc gagaaagagc ggaatcccgc ttccgtcggc
     1801 gaccatgttc agcacgaagc gcttcaggtc ggttctgccg tccttggcgt ggccgaacgc
     1861 gatctcccat ttgaacccct cgttcggaac gtactcgccg tgcacgctga aattcgtcgt
     1921 gtcgacgtga agattgacgg ttccatggag caagggggcg agacattcga ggacgaaacg
     1981 ctcgtagaga acggagatgt tgtattcgct caggttatcg agaagacgtc ccacggcgtc
     2041 gtcgttcaga tgctccggtt gtacgcccgg cc
//
LOCUS       DAOPKU010000546         2067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_63118_length_2067_cov_2.757455, whole genome shotgun sequence.
ACCESSION   DAOPKU010000546 DAOPKU010000000
VERSION     DAOPKU010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63118_length_2067_cov_2.757455"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PK849_13375"
     CDS             <1..271
                     /locus_tag="PK849_13375"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPE67165.1"
                     /translation="GGLAGSQCMTDKAPRMFSGDYSPGGKMWLHVKDLKNVMTACREY
                     DVAMPLTESVLKMMEEVVRDTGPDTDHGSVAKYFEKLNGVSLVTK"
     gene            complement(466..>2067)
                     /locus_tag="PK849_13380"
     CDS             complement(466..>2067)
                     /locus_tag="PK849_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPE67166.1"
                     /translation="VGDYLTLLHLGSSPEEILRHIDLEKAPDPCADVDAAIRTEDGRS
                     RFLIVENEEELRSVLDSPLELWRVFLHPSQRRLVEKDWNGPVRVLGGAGTGKTVAAVH
                     RACRLARAIPPGSEEKVLVTTFTVNLASDLAANLARICPQDVMERIEVVNLDRWVYRF
                     LVSHGRTPNFLFDDAKRREFWEDSIARAPAPAGPGFSEAFYREEWERIVLGEEICSEE
                     EYLCADRRGRGVALDERKRRAVWPVFARFRELLTEHGFCEPDDGMREAGKILEGGGER
                     LHYRSIVVDEAQDLGPSAFRLLRAMVPEGPNDLFITGDPHQRIYRHPVVLSRCGISVA
                     GRSCKLLVNYRTTEETRRWAVSLLDGRSLDDLEGGEDDLKGYRSLLRGQEPRLEGFGS
                     FDEEADFLVAFLKEIESKGERLKDVCLVVRSRALLERYREALAGRGLPLVKLEAGGPE
                     NRDEDGVRTATMHRVKGLEFDRMILAGVCEDIVPPKSWREHEDPPMMNELETRERSLL
                     HVAATRARTHILVTWHGEPSPLLGL"
BASE COUNT          392 a          726 c          610 g          339 t
ORIGIN      
        1 gggagggctt gccggaagcc agtgcatgac ggacaaggcg cctcgcatgt tcagcggcga
       61 ctactctccc ggcggcaaaa tgtggctcca tgtcaaggac ttgaagaacg tgatgaccgc
      121 ctgccgcgag tacgacgtcg ccatgccgct gaccgaaagc gttctgaaga tgatggagga
      181 ggtcgtgcgg gacacgggcc ccgacactga ccacggcagc gtcgccaagt acttcgaaaa
      241 gctcaacggc gtctcgctgg tgacgaaata ggtggtgccg ctggcagcgt cacaaccggg
      301 aaggcttgag tcaacgcaca ccgctcgttc cctttgacct ctatgctgaa acaggtaata
      361 tttattacca ccttaggcaa atatcgataa aatttacggc aggctgatgc gaaatgctgg
      421 aaacagcaca gtccctgatt ctcagcgtcg tcaggctctt ccgctttaca gccccagcag
      481 cggggagggt tcgccgtgcc atgtcaccag gatgtgcgtc cgcgccctcg tcgcggcgac
      541 gtggagcagg gagcgctcgc gggtttccag ctcgttcatc atcgggggat cctcatgctc
      601 ccgccacgac ttcggcggga cgatgtcctc gcagacgcct gcgaggatca tccggtcgaa
      661 ctcaagcccc ttcacccggt gcatcgtcgc cgtccggacg ccatcctcgt cgcggttttc
      721 gggccccccg gcctccagct tcaccagagg aagacctcgc ccggcaagcg cctcgcggta
      781 acgttccaga agcgcccgcg agcggacgac caggcagacg tccttcaatc gctccccctt
      841 ggactcgatc tccttcagga acgcgaccag aaaatccgcc tcctcgtcga acgatccgaa
      901 cccctccaga cggggctcct gaccgcgcag cagcgaacgg taccccttca ggtcgtcctc
      961 ccctccctca agatcgtcga gagaacgccc gtccagcagg gagaccgccc agcgcctggt
     1021 ctcctcggtg gtgcggtagt tcaccagcag cttgcagctc cgcccggcga cggaaatgcc
     1081 gcagcgcgag agcacgaccg gatgacggta tatcctctgg tgcgggtcgc ccgtgatgaa
     1141 gagatcgttg ggcccctccg gaaccatggc gcgcagcaga cgaaaagccg acggccccag
     1201 atcctgcgcc tcgtccacca cgatgctccg gtagtgaaga cgttcgccgc cgccctcaag
     1261 aatcttcccg gcctcgcgca tgccgtcgtc gggctcgcag aaaccgtgct ccgtcagcag
     1321 ctcgcggaaa cgcgcgaaga cgggccagac ggcccttctc ttccgttcgt cgagggcgac
     1381 gccccggcct cgacggtcgg cgcagaggta ctcctcctcg gagcaaatct cctcgccgag
     1441 cacgatgcgc tcccactcct cgcggtagaa cgcctcggag aagcccggcc ccgcaggcgc
     1501 aggcgcacgc gcgatcgagt cctcccagaa ctccctccgt ttggcgtcgt cgaacaggaa
     1561 gttcggcgtc cggccgtgcg acacaaggaa gcggtacacc cacctgtcga gattgaccac
     1621 ctcgatgcgc tccatcacgt cctgagggca gatgcgggcc aggttcgccg ccaggtccga
     1681 cgccaggttg acggtgaagg tggtcaccag caccttttcc tccgaaccgg gcggaatcgc
     1741 ccgcgcaagc ctgcacgccc ggtggactgc ggcgacggtc ttccccgttc ccgcgccccc
     1801 cagcacacgc acaggcccgt tccagtcctt ctcgacaagc cgcctctgcg acgggtgcaa
     1861 aaagacgcgc cacagctcca gcggcgaatc gagcaccgaa cgcagctcct cctcgttctc
     1921 cacgatcagg aagcgcgaac gcccgtcctc agtcctgatg gccgcgtcga catccgcgca
     1981 ggggtcggga gccttctcca ggtcgatatg gcgcaaaatc tcctccggcg aggagcccag
     2041 gtgcagcagc gtcagatagt cgcccac
//
LOCUS       DAOPKU010000547         2063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_63263_length_2063_cov_5.830179, whole genome shotgun sequence.
ACCESSION   DAOPKU010000547 DAOPKU010000000
VERSION     DAOPKU010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63263_length_2063_cov_5.830179"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..822)
                     /locus_tag="PK849_13385"
     CDS             complement(43..822)
                     /locus_tag="PK849_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPE67167.1"
                     /translation="MKILFIGDIMGSPGRKAVAAFLPQLSEREGGFDFVLANCENAAA
                     GKGITQKIVNELFAMGISGMTSGNHIWDKSEGVPVLAAESRIVRPANYPDVCPGQGAM
                     LLENGERRLMVANLQGRVFMPPLDCPFRKADELVAAAGDVPLVIDFHAEATSEKKALG
                     YYLDGRVAALLGTHTHVQTADEEILPQGTAYITDAGMTGGHRSSIGMKLEGVLPRYLT
                     GMPSKFEVSGDGLAFNGVVITLDDETGRAVGITRIKERLSH"
     gene            complement(828..>2063)
                     /gene="rny"
                     /locus_tag="PK849_13390"
     CDS             complement(828..>2063)
                     /gene="rny"
                     /locus_tag="PK849_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPE67168.1"
                     /translation="DRVAHREEELKAKQEELENRMLELDEIKKKQLERLEEIARMTSE
                     EARELLLRQTEEDAQHFLGLRIKELEEKTKREADRKAREIIVTAVQRCAVEHTSESAV
                     SVVHLPSDEMKGRIIGREGRNIRTFETLAGVDLIVDDTPEAVTLSSFDPVRREIARLS
                     LERLVTDGRIHPARIEELIEKATKDVENQIMEAGESALLETGVKNMHPELVRTLGQLK
                     FRFSYGQNALQHSLEVAYVSGMIAAELGIDEEIARRAGLLHDLGKAVDHQVEGPHALI
                     GADLARRFNESPEVVSAIASHHEDQEAKSIYDVIVASADAVSASRPGARRESLDAYVK
                     RLEKLEELAKTFNGVSKAYAIQAGREIRVVVTPNITDDAIVHKIAYDIARKIEEEMKY
                     PGQIKVTATKETRATEYAK"
BASE COUNT          369 a          637 c          523 g          534 t
ORIGIN      
        1 gcatatgcgc ctccctcttt cgtctttgtc cttcagtcgt tttcagtgag acagtctttc
       61 tttgattctc gttatgccga ccgctcttcc ggtttcatca tcgagcgtaa taacaactcc
      121 gttgaatgca aggccgtccc cggaaacttc aaacttggaa ggcatccccg tcaggtacct
      181 cggaagaacg ccttcgagtt tcatcccgat cgagcttctg tgcccgccgg tcataccggc
      241 gtccgtgatg tatgcagttc cctgcggaag tatctcctcg tcggcggtct ggacgtgcgt
      301 atgcgttccc aagagggctg cgactctccc atccagatag tacccgagcg cttttttttc
      361 agaagtcgcc tccgcgtgga aatcaatcac gagcggaaca tcgcccgctg cggccacaag
      421 ttcatccgct ttgcgaaagg ggcaatcgag gggaggcata aaaacccttc cctgcaggtt
      481 cgccaccatc aggcgtcgtt ctccgttctc aagcagcatc gccccctggc ccggacatac
      541 gtccgggtag ttcgccgggc gaacgatcct cgattccgcg gccagcaccg gaacgccttc
      601 gctcttgtcc cagatatgat tgcctgaagt cattccggag atccccatgg cgaacagctc
      661 attgacaatt ttctgggtaa tgcctttccc ggcggcggca ttttcgcagt tggcgaggac
      721 aaaatcaaat cctccctcgc gctccgacag ctgcggaaga aaggcggcga cagcctttct
      781 tcccgggctt cccatgatat cgccgataaa aagaatcttc atagcggtta cttcgcgtac
      841 tccgtggcgc gcgtctcctt cgtcgctgta acttttatct ggccgggata cttcatctct
      901 tcttcaatct ttcgggcaat atcgtacgca attttatgca cgatcgcatc gtcggtaata
      961 ttcggggtca ccaccacgcg aatttcgcgc ccggcctgga ttgcgtacgc cttgctgact
     1021 ccgttgaacg tctttgcgag ttcctcgagt ttttcaagac gtttgacata ggcgtcgagg
     1081 ctttcgcggc gcgccccggg acgcgatgcg cttactgcgt ccgcggaggc gacgatgaca
     1141 tcgtagatgc ttttcgcttc ctgatcttcg tgatgcgaag cgatagcgct tacgacctcg
     1201 ggactttcgt tgaaccgtcg cgcaagatcc gcgccgatca gagcgtgggg accttctacc
     1261 tgatgatcca cggccttgcc cagatcatgg agcagaccag cccgacgggc gatttcctcg
     1321 tcaatgccca gttcagcggc gatcattccg gacacgtacg ccacttcaag gctgtgctga
     1381 agcgcatttt gcccgtagct gaaccggaat ttgagctgac cgagagttcg taccagctcc
     1441 gggtgcatat ttttgacgcc ggtctccagc agagcgctct cgccggcctc cataatctga
     1501 ttctccacat ccttggttgc cttctcgata agctcctcga tcctggccgg atgtattctt
     1561 ccgtcggtca cgagacgttc cagcgacagt ctggcgatct cgcggcgcac agggtcgaag
     1621 ctgctcagcg taacggcttc cggtgtgtcg tcgacgataa ggtctactcc cgccagggtt
     1681 tcgaaggtac ggatgttccg tccctctctg ccgataattc ttcccttcat ctcgtccgag
     1741 ggaagatgca ccacgctcac tgccgattcc gatgtgtgct cgactgcaca gcgctgtacc
     1801 gccgtcacga taatctcacg cgcttttctg tccgcctcgc gctttgtttt ctcttcaagt
     1861 tccttgatac gaagaccgag gaaatgctgc gcatcctcct ccgtttggcg aagaagtaat
     1921 tcccttgctt cctcgctcgt catgcgggcg atttcttcga ggcgttccag ctgtttcttt
     1981 ttgatttcgt cgagttcgag catcctgttc tccagctctt cctgctttgc cttcaactcc
     2041 tcttcacggt gggcaacgcg gtc
//
LOCUS       DAOPKU010000548         2061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_63375_length_2061_cov_3.888833, whole genome shotgun sequence.
ACCESSION   DAOPKU010000548 DAOPKU010000000
VERSION     DAOPKU010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63375_length_2061_cov_3.888833"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK849_13395"
     CDS             <1..240
                     /locus_tag="PK849_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67169.1"
                     /translation="GVDPHGYVSRERSPDEGLPWDHISAGVAKEFLWRERLRSREEKL
                     SPDCREDGKCAACGAACLACGACFPPSQEGGAPRA"
     gene            233..940
                     /locus_tag="PK849_13400"
     CDS             233..940
                     /locus_tag="PK849_13400"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HPE67170.1"
                     /translation="MLRVRLTYAKRGRACFIPHIAIPSVFSRSGYRAGISFQLSEGFS
                     PRPRISLGPELPVGVPALSEPLEVRLLSFSADELIRWDRALPPGFFFTGACVVTGEPG
                     SAEAKSLNKWCEASSCILALREEPAGASPLESFLQEMKDLGDVLAFERAEPLGFRCVL
                     ENPAQRSPGFLVKALQKRGIIEGWPDIFILREAVGRLDMAEESGTAHVLPLTHPLSEE
                     FEQALEKVATGESVFSE"
     gene            1162..>2061
                     /locus_tag="PK849_13405"
     CDS             1162..>2061
                     /locus_tag="PK849_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HPE67171.1"
                     /translation="MRGGSVAGNADRKIIANCIDPEETRVAIIEDGRLADLFVERMWE
                     RQKAGEIYKARVESVLPGIHASFVNLGDGRNAFLYLNDARGLDLQPNKELLVQVTKTA
                     RKNKGARVTSRISLPGRYVVLVPGGQESGVSKRIVDENERKRLKAIARELRGDYGIIV
                     RTAAEGVDDESLAHDVETLLALWREIEHTGRTQSAPCLLYRDLGLLGRVLRDELTEQV
                     SEIIVDGPEEFENVGSHLSSLCGGEPPELSLYKGTIPLFEHYGVEKEIDAALERKVWL
                     KSGAYLIIDHTEALTVIDVNTGKY"
BASE COUNT          405 a          592 c          685 g          379 t
ORIGIN      
        1 ggtgtggatc cgcacggata cgtctccagg gagcgctcgc cggacgaggg gcttccctgg
       61 gatcacatct ccgccggcgt cgcgaaggag ttcctctgga gggagcgtct gcgctcccgc
      121 gaagagaaac tctctccgga ctgccgcgag gacggaaagt gcgccgcgtg cggcgccgcc
      181 tgcctcgctt gcggggcatg ttttcctccc tctcaggaag ggggcgcgcc ccgtgcttag
      241 agttcgcctg acctacgcga agcggggaag agcgtgcttc attccgcata tcgccatacc
      301 ttcggtcttc tccagaagcg gataccgcgc tggaatctcc ttccagcttt cggaggggtt
      361 ttccccgcgg ccgcgcatca gcctcggccc ggagctccct gtgggtgttc ccgcgctgag
      421 cgagccgctg gaagtccgtc tgctctcctt ttcggcggac gaacttatca ggtgggacag
      481 agcgcttccc ccgggttttt tcttcacagg ggcgtgcgtt gtgaccggag aacccgggtc
      541 tgcggaggcg aagtcgctga acaagtggtg cgaggcttcc tcgtgcatcc tcgctctccg
      601 ggaggagcct gccggcgcat cgccgctgga atccttcctg caggagatga aggacctggg
      661 ggacgtgctt gctttcgagc gggcggagcc cttgggcttc aggtgcgttc tggagaaccc
      721 cgcgcaaagg agtcccggct tcctggtgaa ggcgctccaa aaaagaggta tcatagaggg
      781 gtggcccgat atcttcatcc tgagggaagc ggtggggcgg cttgacatgg cggaagagag
      841 cggaacggcg cacgtgcttc cgctgacgca tcctctctcc gaggagttcg agcaggcgct
      901 tgaaaaggtt gcaaccgggg agtccgtatt ttcagagtga ggttccccgg agtatagata
      961 aagaaagcgg cgggaatttt ctccttctca tgaaaaagag aaggagattc gtcctgttgt
     1021 acatgtttgt ccgaaaagag gagggagttg cgtttgtcag agaatgcgga ggccagaagc
     1081 gaacgcgcgg aatcgcgcga cgctgtgata ccccgtaccg aagcgaggga ggacgtcgat
     1141 ccgcaggagg gaaggcccgg tttgcgggga gggtccgtcg ccggcaacgc cgacaggaag
     1201 attatcgcga actgcatcga ccccgaagaa acgcgggttg ccatcatcga ggacggccgt
     1261 ttagccgacc tgttcgtcga gcgcatgtgg gagcgtcaga aggcgggaga gatctacaag
     1321 gccagggttg agagcgtgct ccccggcatc cacgcttcct tcgtcaacct tggcgacggg
     1381 agaaacgcct tcctctacct caacgacgcc agagggcttg atctccaacc gaacaaggaa
     1441 cttctcgtcc aggtgaccaa gaccgccagg aagaacaagg gggcgagggt gacctccaga
     1501 atctccctcc ccgggcggta cgtcgtgctc gttcccggcg ggcaggagtc cggagtctcc
     1561 aagcggatcg tcgatgagaa cgagcgaaaa cgcctgaagg ccatcgcccg cgagcttcgc
     1621 ggcgactacg gtattatcgt ccggacagct gcggaagggg tggacgacga atcgctcgcc
     1681 cacgatgtgg agacgctgct cgctctgtgg cgcgagatag agcatacggg gcggacccag
     1741 agcgctccct gccttctgta cagagatctc ggcctgctgg ggagagtcct gcgggacgag
     1801 ctcaccgaac aggtttcgga gatcattgtg gacggccccg aagagttcga gaacgtcggc
     1861 agtcaccttt cgagcctgtg cgggggcgaa cccccggagc tttcgctgta caagggaaca
     1921 atcccgctct tcgagcacta cggagtggag aaggagatcg acgccgccct cgaacgcaag
     1981 gtgtggctca aatcgggcgc ttacctgatc atcgaccaca cagaggcgct gaccgttatc
     2041 gacgtcaaca ccggtaaata t
//
LOCUS       DAOPKU010000549         2057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_63557_length_2057_cov_3.937063, whole genome shotgun sequence.
ACCESSION   DAOPKU010000549 DAOPKU010000000
VERSION     DAOPKU010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63557_length_2057_cov_3.937063"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PK849_13410"
     CDS             <1..973
                     /locus_tag="PK849_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE67172.1"
                     /translation="EDASALEREAYALKGVAPEIYSRTQLVYGNMNWSTLVTGSTSAI
                     FDIREWKIADGRFYIDDEARGGAKIVVLGATVAKNLFGTEDPIGKIVRIRNVPMEVVG
                     VLAAKGQTPWGQDQDDTAFVPFATAQRSLFRRGIANSVQRITVAAVNGDSVPEAEREI
                     NAILRQRRRLNEGEASDFDVRNMAEMLDAAAQSTKVMSLLLGAVASVSLLVGGIGIMN
                     IMLVSVTERTREIGIRMAIGARASDIRTQFLMEALLLSIAGGIIGIVLGMSASRLITG
                     FLGWPTVVSTGSIVLAFGFSVFVGIFFGFYPAWKASLLNPIEALRYE"
     gene            complement(1064..>2057)
                     /locus_tag="PK849_13415"
     CDS             complement(1064..>2057)
                     /locus_tag="PK849_13415"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPE67173.1"
                     /translation="FRRLLADERLFLKFLRRFLRGSLPGDIDLDSLTVEDVELQNITF
                     IPPDLSKNESDVLYKVRRGSAEMYVYILVEHQSRVDFMMPFRLLSYMVEFWKRCTEQA
                     GSRARRAGFLLPPVLPVVFYEGASKWTAPVRFADKVESSSDFRGLVPDFEYLLISLRD
                     KRSEDLLSFRDALGGLCYLAAPSKKESFAEAAERLRMLLTVLSDEETELFTNHLRGYL
                     RILMKKEGLEIDDALDLCLEREEADEMLTYMLKEFRKTRKEGREEGREEGREEGLEEG
                     LEKGQLEMGISIALAMLEAGEPEEKILRYTGFSSVQLAEIRDGRKSGGKKPSSR"
BASE COUNT          384 a          668 c          614 g          391 t
ORIGIN      
        1 agaggacgcc tccgccctgg agcgcgaggc gtacgcattg aagggcgtgg cccccgagat
       61 ttacagccgt acgcagctcg tctacggcaa catgaactgg tccaccctgg tgacggggag
      121 cacgtccgcc atcttcgaca tccgcgagtg gaagatcgcc gacgggcgct tctacatcga
      181 cgacgaggcg agggggggcg cgaagatcgt cgtgctgggg gcgacggtcg caaagaacct
      241 cttcggcacg gaggacccga tagggaagat cgtccgcatc cgcaacgtcc cgatggaggt
      301 cgtcggcgtg cttgccgcca aagggcagac accgtggggg caggaccagg acgacaccgc
      361 gttcgtcccc ttcgccacgg cgcagcgcag cctcttccgg cggggcatcg ccaactccgt
      421 ccagcggatc accgtcgcgg cggtcaacgg cgactcggtt cccgaggcgg agcgcgagat
      481 caacgccatc ctccgtcagc ggcggcggct caacgaagga gaggcgagcg acttcgacgt
      541 gcgaaacatg gcggagatgc tcgacgccgc ggcgcagtcc acgaaggtga tgtcgctgct
      601 gcttggggct gtggcgtccg tctcgctgct ggtggggggc atcggcatca tgaacatcat
      661 gctcgtctcc gtcaccgagc gcacccgcga aatcggcatc cgcatggcca tcggcgcgcg
      721 cgcctccgac atccgcaccc agttccttat ggaagcgctc ctcctctcca tcgcgggggg
      781 aatcatcggc atcgtgctgg ggatgtcggc ctccaggctc atcacaggct tcctcggctg
      841 gccgacggtc gtctccacag gctccatcgt gctcgccttc ggcttctccg tcttcgtggg
      901 gatcttcttc ggcttctacc ccgcgtggaa ggcctcgctg ctgaatccga tagaggcgct
      961 gcggtacgag taggggacga aacagagcaa aagcgcgccc ttcctctctg attggaaggg
     1021 cgcgcctttg ttcacattgc tgcttcacgc cgactaggtc gggttacctg gacgagggtt
     1081 ttttcccgcc ggacttccgg ccgtcgcgta tctctgccaa ctgcacggag gagaagccgg
     1141 tatagcgcag tatcttctcc tcaggctcgc ccgcttcgag catcgccaga gctatggaga
     1201 tccccatctc gagctgccct ttctcaagcc cttcctcaag tccttcttcg cggccttctt
     1261 cgcggccttc ttcgcggcct tccttgcgag tcttcctgaa ctccttcagc atgtacgtca
     1321 gcatctcgtc agcctcctca cgttccagac agaggtccag agcgtcgtcg atctccaggc
     1381 cttccttttt catcagtatc ctcagatagc cgcgcagatg gttcgtgaac agctccgtct
     1441 cctcgtcgga caggacggtc agcagcatcc gcaatcgctc cgcggcttcc gcaaagctct
     1501 ccttcttgga cggggccgcg aggtagcaca gcccgccgag cgcatcgcga aatgagagca
     1561 aatcctcgga gcgcttgtcc cgaagcgata tcagcagata ctcgaagtcg ggaaccagac
     1621 cgcgaaagtc ggacgaactc tccaccttgt ccgcgaaacg caccggagcc gtccatttgg
     1681 aagctccctc gtaaaatacc accggcagaa cgggtgggag aagaaatccg gcccttctcg
     1741 ccctcgatcc ggcctgctca gtgcagcgtt tccagaactc caccatgtat gaaagcaggc
     1801 gaaacggcat catgaagtcc acacgggact gatgctccac cagtatgtag acgtacatct
     1861 ccgcgcttcc ccgccgcacc ttgtagagga cgtcggactc gtttttcgag aggtcgggcg
     1921 ggatgaaggt gatattctgc agctcgacgt cttcgacggt gagcgagtca aggtcgatgt
     1981 cgccgggcag gctgcctctc agaaaccgcc gaagaaactt cagaaagagc cgttcgtcgg
     2041 ccagaagcct gcgaaaa
//
LOCUS       DAOPKU010000550         2047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_64027_length_2047_cov_3.600402, whole genome shotgun sequence.
ACCESSION   DAOPKU010000550 DAOPKU010000000
VERSION     DAOPKU010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64027_length_2047_cov_3.600402"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>2047)
                     /gene="fusA"
                     /locus_tag="PK849_13420"
     CDS             complement(119..>2047)
                     /gene="fusA"
                     /locus_tag="PK849_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPE67174.1"
                     /translation="AATMDYMDQERERGITITSAATTCMWHDHYINIIDTPGHVDFTV
                     EVERSMRVLDGAVAVFCAVGGVEPQSETVWRQADKYHVPRVAFVNKMDRVGADFENVV
                     RGIKEKLGAKPLPVQIPIGVEDSFTGMIDLVEFHGIQYTDDMGTDPHVVPIPSALADE
                     AKAARESMLEILSDYDEQIMEAFLDGGDVPVETIKRAIRAATVALKLVPVICGSAFKN
                     KGVQFLLDAVVDYLPSPVDLPPIKGTEPATEEEVLRQSSTEESFTALAFKIAVDPFVG
                     RLVFTRIYSGVLSKGTAIYNSSTHSRERVGRILRMHANKREELDEATAGMIVALPGLK
                     NTKTGDTLCEEDKPLLLEKLVFPEPVIHLSVEPATKNDKIKLTKGLSALSEEDPTFRA
                     YTDEETSQTIISGMGELHLEIIVDRLKREFGVDVKVGRPQVAYREAIKHAARAEGKYV
                     RQSGGRGQYGHVVFEVEPLEEGKGFEFEDKIVGGVVPKEYINAVSKGLEEALNNGVLG
                     GYPVIGIKVALVDGSYHEVDSSEMAFKIAASMGFKEAMRKAGPVLMEPIMSVEVVTPE
                     DYVGDVIGDLSSRRGRIDGMDMRMNTRIVRAFVPLAEMFGYSTDLRSKTSGRAAYSMQ
                     FHHYEAVSSDVADKVLKG"
BASE COUNT          402 a          606 c          559 g          480 t
ORIGIN      
        1 tcgtacctat gttcaaatgc ggcttcgacc tctcaaattt ctccttcgcc attgttcaat
       61 tccctcctcg gaaaaatcat tattgctttg aatcaaaatc aataagtata tgcgttcctc
      121 agcccttgag caccttgtcg gcaacgtcgg aactgaccgc ctcgtagtga tggaactgca
      181 tggaatacgc tgcgcgtcct gaagtcttgc tccgaaggtc ggtggaatac ccgaacatct
      241 cggcgagagg aacgaatgca cggactatcc tggtgttcat tcgcatgtcc attccatcga
      301 ttcgtcccct ccgggaggag aggtcgccga tgacgtcgcc tacgtagtcc tccggggtga
      361 cgacttcgac tgacatgatg ggctccatga ggaccggtcc cgccttccgc atagcctcct
      421 tgaatcccat ggaggctgcg attttgaagg ccatttcgga gctgtccact tcgtggtagc
      481 ttccgtcgac gagcgctact ttgatgccga tcaccgggta tccgccgagg acgccgttgt
      541 tgagcgcctc ttcaagtccc ttggagactg cgttgatata ctccttggga acgacgccgc
      601 ccacgatctt gtcctcgaat tcgaagcctt tgccctcttc gagcggctcg acctcgaaga
      661 caacgtggcc gtactggccg cgtccgcccg actggcggac atatttcccc tcggctctgg
      721 cagcgtgctt aatagcttcg cggtaggcga cctgaggtct tccgaccttg acgtcgacgc
      781 cgaactctct tttgagacgg tcgacgatga tctccagatg aagctcgccc atgccggaaa
      841 tgatggtctg agaggtttcc tcgtccgtat aagcgcggaa tgtggggtct tcttcggaca
      901 gcgccgagag ccccttcgtc agcttgatct tgtcgttctt cgtcgcgggt tcgaccgaga
      961 ggtggataac cggctcgggg aaaaccagct tctcaagaag aagcggtttg tcctcttcgc
     1021 agagggtatc tccggtcttc gtgttcttca gccccggaag cgcgacgatc atgcctgcgg
     1081 tggcttcgtc cagctcttcc cgcttgttgg catgcatcct caggatgcgc cccacgcgct
     1141 cgcggctgtg tgtgctggag ttgtagatgg ccgtcccctt cgaaaggacg ccggaataga
     1201 tgcgcgtaaa gacgagccgt ccgacgaaag gatcaaccgc aattttgaat gcaagcgcag
     1261 tgaaggactc ctcggtgctg ctttgtctga ggacctcctc ttccgtcgcg ggttccgtcc
     1321 ccttgatggg gggaaggtcc accggactcg gaagatagtc cacaacagcg tcgagcagga
     1381 actgaacccc tttgttcttg aacgccgatc cgcagatgac cggaacgagc ttcagtgcga
     1441 cggtagcggc gcgaattgct ctcttgatcg tttctacagg gacgtccccc ccgtcgagaa
     1501 aagcttccat gatctgctcg tcataatccg aaagaatctc cagcatggat tcccgtgcgg
     1561 ccttggcctc gtcggcaagc gctgaaggaa taggtacaac gtgaggatcg gttcccatat
     1621 cgtccgtgta ctggatacca tgaaattcga caagatcgat cattcctgta aagctgtcct
     1681 cgacgccgat cggaatctgg acgggaagcg gcttggctcc aagcttttcc ttgatgcccc
     1741 ggacgacgtt ctcaaaatcg gccccgacac gatccatctt gttcacgaag gcaactctcg
     1801 gcacgtggta cttgtccgcc tgacgccaga ccgtctcgga ctgcggctcg actccgccga
     1861 cagcacagaa gaccgctacc gcgccgtcga ggactctcat tgaacgctcg acctcgacag
     1921 tgaaatccac gtgcccgggc gtatcaatta tattgatata gtgatcgtgc cacatgcacg
     1981 tcgtagcagc ggaggtaatg gtgatccccc gctcccgttc ctggtccatg tagtccatgg
     2041 tggcggc
//
LOCUS       DAOPKU010000551         2022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_65165_length_2022_cov_2.880020, whole genome shotgun sequence.
ACCESSION   DAOPKU010000551 DAOPKU010000000
VERSION     DAOPKU010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65165_length_2022_cov_2.880020"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1656)
                     /locus_tag="PK849_13425"
     CDS             complement(145..1656)
                     /locus_tag="PK849_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPE67175.1"
                     /translation="MNTAKLPDVEQIAPLIRGKVSLDALKAAGILPIREDEERFVVAL
                     SSLEAFPNAQVLAASFGKEADWEVHSRESLDVMLGRLYDLRGGVGDDTLSAMTGIDDD
                     DDLMREDVQSDSVDVPVIKLVNGLFREALKIRATDIHVEPCENEVLIRFRVDGVLHDR
                     LRLPRNQQAPLTSRIKVMARMDIAERLAPQDGRIGITVGKRAVDIRVNSIPVQHGERI
                     ALRILDKGAGLLSLEQLGMDATSREAMESLIQIPHGLILFTGPTGSGKTTTLYAILQA
                     LAKPAVNIITVEDPIEYAVPGVGQIQVNEKAGVTFPSALRSILRQDPDIVMIGEMRDY
                     ETAHIGVQASLTGHLVLSTLHTNDSISAVVRLIDMGIEPYLAAGSLLGAIAQRLVRTN
                     CPECIEEFEAPSLFRKEGIHKVMRGRGCPACMGTGFKGRVGLYEQFLLDDSLREAVAS
                     NLPQSRIRAEARKHGFRTLWELGLDALREGRTSPEELLRVVSAGEFASDEGGD"
     gene            complement(1676..>2022)
                     /locus_tag="PK849_13430"
     CDS             complement(1676..>2022)
                     /locus_tag="PK849_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HPE67176.1"
                     /translation="ITSKRKIKTNVMVASGSTLILGGLIKEAEKSIKSRVPGLSYIPL
                     IGGLFSRSVKQREKIELMIFLTPYILETPQEAEEMTREIVAGEHGPSAPERSLWERME
                     KEYRESIKKQSK"
BASE COUNT          398 a          608 c          578 g          438 t
ORIGIN      
        1 ggccgcccct tcgatgatct caacggccac caggttctgc tctgcgagga gttcacctgc
       61 gcgggtgctc gacggcgcgt cgataatccc cttgcggagc tttcccgagg tatcgtatgc
      121 agagtagcga taagaaggca tctatcagtc tcctccctcg tccgaagcaa attcgccggc
      181 ggacacaacg cgaagcagct cttccggaga cgttctccct tcgcggagcg catcgagccc
      241 aagctcccag agcgtcctga aaccgtgttt gcgggcctcg gcgcgtattc tggactgcgg
      301 gaggttggag gccactgctt ccctgagact gtcgtccagg agaaactgct cgtaaagacc
      361 gacacgcccc ttaaaaccgg tacccataca tgcggggcag cctctccccc gcatcacttt
      421 gtgtatgcct tccttccgga agagagaggg cgcttcgaac tcctcaatgc actcagggca
      481 gttcgtccgg acaaggcgct gcgcaatggc tccgagaagc gatcctgccg cgagataggg
      541 ctcaatcccc atgtcgatca aacgcaccac ggccgagatc gaatcgttgg tgtggagcgt
      601 cgagaggaca agatgtcctg tcagggacgc ctgcacaccg atgtgcgccg tttcgtagtc
      661 gcgcatttcg ccgatcatca caatatcggg gtcctggcgc agaatcgaac gcagggcgga
      721 cgggaaggtc accccagcct tctcgttcac ctggatctgc cccacgccgg gaacggcgta
      781 ttcgataggg tcttcgacgg tgatgatgtt caccgccggc ttcgccagag cctgcaagat
      841 agcgtagagc gtcgtcgtct ttcccgatcc ggtagggccg gtgaagagga taaggccgtg
      901 cgggatctga ataagacttt ccatcgcctc tcgtgaagtg gcgtccatac cgagctgttc
      961 cagagagagc aacccggcgc ccttgtcaag aatacggaga gcgatgcgct ctccgtgctg
     1021 gacggggata gagttgacgc ggatgtccac agcacgtttt ccgacggtga tgccgatgcg
     1081 tccatcctgc ggagcgaggc gctcggcaat gtccattctc gccatcacct tgatacggct
     1141 ggtcagaggg gcctgttggt ttctgggaag gcggagccgg tcgtgaagca ctccgtccac
     1201 tctgaagcgg atgaggactt cgttttcgca cggttcgacg tggatgtccg tcgcacggat
     1261 cttcagcgcc tcacggaaga ggccgttcac cagtttgata accggtacgt ccacggagtc
     1321 gctctgaaca tcctcccgca tgagatcgtc gtcgtcgtcg atgccggtca tcgcggagag
     1381 cgtatcgtcg cccacgcctc cgcgcaaatc gtagagacgg ccgagcatga cgtcaaggga
     1441 ttcccgggaa tgcacctccc aatccgcctc tttaccgaac gaggcggcaa gaacctgtgc
     1501 attcgggaac gcctccaacg acgagagcgc caccacaaag cgctcttcat cctccctgat
     1561 aggcagaata ccggcagctt tcagtgcatc aaggctcact ttcccgcgta tgagcggcgc
     1621 tatctgctcc acgtcaggga gttttgctgt attcacggct ctattccacc ttccgttatt
     1681 tcgactgttt ctttatagat tctcgatact ccttctccat acgctcccag aggcttcttt
     1741 cgggagcgct tgggccgtgc tcaccggcga cgatctcgcg ggtcatctct tcagcctcct
     1801 gcggggtctc caggatataa ggcgtgagga agatcatcag ttcgatcttc tctcgctgtt
     1861 ttacagagcg tgaaaagaga ccgccgatga gcggaatata tgaaagaccg ggaacgcggc
     1921 tcttgatgct cttctccgct tccttgataa gacctccgag aatgagcgta ctgccggagg
     1981 ccaccatcac gttcgtcttg atctttcgct tggatgtgat gg
//
LOCUS       DAOPKU010000552         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_65191_length_2021_cov_4.557477, whole genome shotgun sequence.
ACCESSION   DAOPKU010000552 DAOPKU010000000
VERSION     DAOPKU010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65191_length_2021_cov_4.557477"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1267)
                     /gene="mglB"
                     /locus_tag="PK849_13435"
     CDS             complement(260..1267)
                     /gene="mglB"
                     /locus_tag="PK849_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HPE67177.1"
                     /translation="MKRIVGLAVLIGICGLFLMAGGVFAADLEIGCAIYKFDDTFMTG
                     VRNAIAEAAKGKAKVDIVDSQNSQSTQNDRVDMFITKRMNALAINPVDRTAAGVIIDK
                     AKAENIPVVFFNREPLPEDMQKWDKVYYVGARAEESGTMSGQIIVDFWKSHPEADKNG
                     DGVLQYVMLKGEPGHQDAELRTEFSIKAVTDAGIKVEKLAEDTGLWDRVRGQEKMAAF
                     LAAHGDKIEAVFGNNDDMALGAIEALKAAGYFSDGKFMPVVGVDATAPAIQALEDKTL
                     LGTVLNDAVNQGVATFNLSYILAKGETPTEENSGYVISDGKYVWVPYRKVTLENVAEF
                     K"
     gene            1506..>2021
                     /locus_tag="PK849_13440"
     CDS             1506..>2021
                     /locus_tag="PK849_13440"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPE67178.1"
                     /translation="MTTSLVCGGAGYIGSHMVRALLERGERVLVLDDLSKGHREYVPD
                     EAVFSKGDIRDGELLDRIFRENAVDAVFHFAAFIQVGESVQRPLEYYENNVTGVLRIL
                     ECMVRHKTPHIVFSSTAAVYGEPDFVPIPEDLPTAPTNPYGETKLAVEKMLRWASAAH
                     GYTYGALRYFNA"
BASE COUNT          388 a          629 c          545 g          459 t
ORIGIN      
        1 actttcctgc gccattctct cccatcagcg catgaactgt ccccgggcgc acttgcagcg
       61 tgacgtcgcg aagcgcctgc acccccggaa atgcctttga aacattccgc atctcaagtg
      121 catacttgtc gtttttttcc acgaagacac cttcccttcg catgcctcta tcgctcccgg
      181 agcacaatgc cagggaacga tagcaaagaa agggaggcgc ttgcccccct ttcaaagctg
      241 cctttccgca aacgacgttc tatttgaact ccgcgacgtt ctccagagtg acctttctgt
      301 agggaaccca gacgtacttc ccgtcgctga tgacgtatcc gctgttctcc tcggtcgggg
      361 tctcgccctt cgccaaaatg taggaaagat tgaaggtcgc gactccctgg ttgactgcgt
      421 cgttcagcac tgtgcccagg agcgtcttgt cctcaagggc ctgaatcgcc ggcgcggtcg
      481 cgtcgacgcc aacaacgggc ataaactttc cgtcgctgaa gtagcctgcc gctttcaggg
      541 cttcgatcgc cccaagcgcc atgtcgtcgt tattgccgaa gaccgcttcg atcttgtcgc
      601 cgtgcgccgc gaggaaggcc gccatcttct cctgaccgcg gaccctgtcc cacagccccg
      661 tatcctccgc gagcttctcg accttgattc ccgcgtcggt taccgccttg atggagaact
      721 cggtacggag ctcggcatcc tgatgccccg gctctccctt gagcatgacg tactgaagga
      781 cgccgtcgcc gtttttgtcc gcttcggggt ggctcttcca gaagtcgacg atgatctgcc
      841 ccgacattgt gccggactct tccgcgcgcg cccccacgta gtagaccttg tcccacttct
      901 gcatatcctc gggaagaggt tcgcggttga agaagactac ggggatgttc tcggccttgg
      961 ccttgtcgat gataacgccg gccgcggtac gatccaccgg gttgatggcg agcgcattca
     1021 tcctcttggt gatgaacatg tcgacccggt cgttctgggt ggactgggag ttctggctgt
     1081 cgacgatatc gactttcgcc ttgcccttgg ccgcctcggc gatcgcgttt ctgacgccgg
     1141 tcatgaaggt gtcgtcgaat ttgtagatgg cgcaccctat ctcaagatcc gccgcaaaaa
     1201 cgccacccgc cataagaaaa agcccgcaaa taccgatgag caccgcaagt ccaactattc
     1261 tcttcacttt cgaaaaacct cctctgcaaa gatttcgcgc catggaactc cacggacgtc
     1321 tgtggggtct ttcccccggt caagtgtagc caagaggagc ctacctgtca attatatttt
     1381 caatttacac tgaaaacgcg cgcccttgac atatcatcgg tcgggggata ccctgttgcc
     1441 caaatccggg gtgcgaactg atccggagtt catactgttt ctcacggctg gagggctgat
     1501 tattcgtgac gacgtccctt gtctgcggcg gtgcaggata tatcggaagt catatggtga
     1561 gagcgcttct cgagcgtgga gagcgggtgc tcgtacttga cgatctctcg aaaggacatc
     1621 gggaatacgt cccggacgag gccgtattct ccaaagggga tattcgggac ggagagctgc
     1681 tcgaccgcat attcagggaa aacgccgtgg atgccgtctt ccactttgcg gcgttcatcc
     1741 aggtcggaga gagcgtgcag cgccccctgg aatactacga gaacaatgtc accggcgtcc
     1801 tgcgcatact tgagtgcatg gtacgccaca agacgccgca cattgttttc tcttccaccg
     1861 ctgcggtcta cggagagccg gatttcgtcc cgatccccga ggacctgccg acggcgccga
     1921 ccaatccgta cggggagacg aagctcgccg tcgagaagat gcttcgctgg gcttccgcag
     1981 cccacggata cacctacggc gccctgcgct atttcaacgc c
//
LOCUS       DAOPKU010000553         2013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_65562_length_2013_cov_6.682329, whole genome shotgun sequence.
ACCESSION   DAOPKU010000553 DAOPKU010000000
VERSION     DAOPKU010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65562_length_2013_cov_6.682329"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1187
                     /gene="aroF"
                     /locus_tag="PK849_13445"
     CDS             141..1187
                     /gene="aroF"
                     /locus_tag="PK849_13445"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPE67179.1"
                     /translation="MMVVEMAEGSTRRDLDRVCESMAGGGRRYSLVSGRGSAYVMSAD
                     AARGEESAPFRLLPGVRSVNVTTCCHPLASREIGGESRPMELAKGLWIGDGEPLVMAG
                     PCAVESRKQLLRTAEGVQRAGARVLRGGAFKPRSHPYAFQGLGSEGIALLKEARAATG
                     LPVVTEVMSSEEAEWVAPHIDILQVGARNMQNFSLLRTLGGLDRPVLLKRGMCATIEE
                     WLQAAEYILAGGNERVILCERGIRSFDKNTRNTLDLSVVPLLKSMTHLPVVVDPSHAT
                     GRRELVAPMSLAALAAGADGLVVEVHCDPAEALCDGPQSLTPDDFALLMTRASKMLRA
                     LNEEEPSWRMPVAR"
     gene            1163..>2013
                     /locus_tag="PK849_13450"
     CDS             1163..>2013
                     /locus_tag="PK849_13450"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HPE67180.1"
                     /translation="MAHAGCQMRTALVEETHDALLQGRGAFPTNVVVGERLLSRLSDF
                     TGSEEAPFVVADGITGPLFAEFLGTRRGLFTLPRGEEGKNLENLSKIYAALAAAGLDR
                     SGTLLALGGGVVGDTAGFAAATWMRGIRLLNCPTTLLAQVDSAIGGKTGVNLPEGKNL
                     VGAFHPAEWVFSDVECVRSQADEDFRQGLGEAVKYGVGEEWGFFGWLEENARAILRRS
                     LPALRTLVAECSRMKLSVVAEDEHERSGARARLNLGHTVGHALEAASGYRWRHGEGVA
                     AGMMVAG"
BASE COUNT          352 a          615 c          728 g          318 t
ORIGIN      
        1 atatccggcc tctccgggag cagcgcaccg ataggacgag gcggatacca agaaaagaca
       61 aacgccggaa ccgggtttct acacccggtt ccggtttttt tatttcgtgg ctgaatgaat
      121 gtgaaaaggg ggaaggcatc atgatggtgg tcgaaatggc ggagggcagc acgcgacggg
      181 atctggacag agtatgcgaa tcaatggcgg gcggcgggcg gcgttacagt ctcgtctccg
      241 gaagagggag cgcttatgtc atgtcggccg acgccgcgcg cggggaggaa tccgcgccct
      301 tccgcttgct tcccggcgtg cgaagcgtga atgtaacaac gtgctgccac cctctcgcca
      361 gcagggagat cggcggggaa tcccgtccga tggagctcgc gaaaggtctc tggatcggcg
      421 acggggagcc gctcgtcatg gcgggcccct gcgctgtgga gagcagaaaa cagctgctga
      481 ggacggctga aggggtacaa cgggcgggag cgcgcgtcct gaggggcggc gcgttcaagc
      541 cccgctcgca cccctacgcc ttccaggggc tggggagcga ggggatcgcg ctgctgaagg
      601 aggcgcgtgc agccacaggg ctgcctgtgg tcacagaggt catgtcctcc gaggaggcgg
      661 agtgggtcgc gccgcacatc gatattctcc aggtgggcgc ccggaacatg cagaacttct
      721 ccctgctgag gacgctcggc gggctggaca ggccggtgct gctgaaacgc gggatgtgcg
      781 ccaccatcga ggagtggctt caggcggcgg agtatatcct cgccggaggg aacgagcgcg
      841 tgatcctctg cgaacgcgga atccgcagct tcgacaagaa cacccgcaac accctcgacc
      901 tgagcgtcgt cccgctgctc aagtccatga cgcacctccc ggtcgtcgtg gaccccagcc
      961 acgccaccgg cagacgggag ctcgtcgcgc cgatgagcct cgcggcgctc gccgccggag
     1021 cggacggcct cgtcgtcgag gtgcactgcg accctgcgga ggcattgtgc gacgggccgc
     1081 agtcgctgac cccggacgac ttcgcgcttc tgatgacgcg ggcgtcgaaa atgctccgcg
     1141 cgctcaacga ggaggagccg tcatggcgca tgccggttgc cagatgagga cggcgctcgt
     1201 ggaagagacg cacgacgcgc ttctccaggg gcgcggagct tttcccacga acgtcgtggt
     1261 cggcgagaga ctgctttcgc gcctgtctga cttcacggga agcgaggagg cgccgttcgt
     1321 cgtcgcggac gggatcaccg ggccgctctt cgcggagttc cttggcacgc ggcgcggact
     1381 cttcacgctc ccccgcggcg aggagggaaa gaacctcgaa aacctctcca aaatctatgc
     1441 cgcgctcgcc gcggcaggcc ttgaccgctc cggaacgctg ctcgccctcg gcggaggcgt
     1501 ggtgggcgat accgcagggt tcgccgccgc cacatggatg cgcggcatcc ggcttctcaa
     1561 ctgcccgacg acgctcctcg cgcaggtgga cagcgcgatc ggcgggaaaa cgggggtgaa
     1621 ccttccggag gggaaaaacc tcgtcggcgc atttcacccc gccgagtggg tcttttcgga
     1681 cgtggagtgc gtccgctccc aggccgacga agatttccgg cagggcctgg gggaggctgt
     1741 gaagtacggc gtcggggagg agtggggatt cttcggatgg cttgaagaga atgcgcgcgc
     1801 catcctgcgc cgctctctgc ccgcactgag gacgctcgtc gcggagtgtt cgcggatgaa
     1861 gctctccgtg gtcgcggagg acgagcatga acggagcggg gcgcgggccc gtctgaacct
     1921 cgggcacacc gtcggacacg cgcttgaagc cgcgtccggc tacagatggc ggcacggcga
     1981 aggcgtcgcc gcggggatga tggttgccgg aag
//
LOCUS       DAOPKU010000554         2005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_65974_length_2005_cov_3.527692, whole genome shotgun sequence.
ACCESSION   DAOPKU010000554 DAOPKU010000000
VERSION     DAOPKU010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65974_length_2005_cov_3.527692"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..325)
                     /locus_tag="PK849_13455"
     CDS             complement(197..325)
                     /locus_tag="PK849_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67181.1"
                     /translation="MEPAVSEFASVPVVAAGAGAKETVRRAYDEEASGISGVFPLS"
     gene            444..1337
                     /locus_tag="PK849_13460"
     CDS             444..1337
                     /locus_tag="PK849_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPE67182.1"
                     /translation="MLMNSRSELRASLRTQLHAAASSQSAETGSVEKPVTDLSAATAS
                     ERALKAAVEEKLSTNRLTEGRAQEQGLFPAARNLFGETEPGKTVSQEGVAQQQAGKPF
                     TILRESLKGEDFSKQTGKEAAESAVYSASPKPLQSASQQQVSFANLLSGETQLQGRGG
                     AALEDGMQHVVRFLRTEGRQAASIIVDPPALGRIEIELVTIAKGVEATIKVASEQVRQ
                     LVQDHITVLRNHLEQQGVHLGEFIVDLRDNSKGQSGRNAFAGERNGRRRGVSVLDAEG
                     IDETIPSFRMDLEHGLLSWVA"
     gene            1350..1766
                     /locus_tag="PK849_13465"
     CDS             1350..1766
                     /locus_tag="PK849_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HPE67183.1"
                     /translation="MAIQNVANSYSAKTTDTALTDAIQNPSTDLGKDAFLKILITQLS
                     NQDPMDPLKDKDFIAQMAQFSTLEQMTNMNKSIEQMVSMNKASAVSYIGRVIEYTDDE
                     GLPTYATVGYVRFEEGKIILNTTDGDVPLEKVISVA"
     gene            1898..>2005
                     /locus_tag="PK849_13470"
     CDS             1898..>2005
                     /locus_tag="PK849_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HPE67184.1"
                     /translation="MSGVSGARGHQTFLDVVGNNIANINTVGFKKSNVTF"
BASE COUNT          493 a          575 c          571 g          366 t
ORIGIN      
        1 atatccgcgc tgcctcgcat cctgtcgata cagaaaccgc cgtacatcgc gcagctgggc
       61 aaacgggcgc tgatatccgc gctgcctcgc atcctgtcga tacagaaacc gccgtacatc
      121 gcgcagccgg gcaaactggc ggtgaaaacc ggcagccgga gaccctcttg cgtccatcat
      181 ttgttcatgc ggcctctcag gaaagcggga aaactccgga aatgccggac gcttcttcgt
      241 cgtatgcccg gcggaccgtc tccttcgccc ccgctccggc cgcgacaacc ggaacggaag
      301 cgaactccga aaccgccggt tccaaagttg aaaatcccgg tgcgccagct ccgttctctt
      361 tgtcgatgac cgacaacgtc aggcccagaa gaagtttttc gctcgctcaa caggaagcgg
      421 cgcccgaaga gactgatgcg gaaatgctga tgaattctcg ttccgaactg cgagcctccc
      481 tgcggacgca gcttcacgcc gccgcttcct cacaatcggc ggagacaggg agcgttgaga
      541 aacccgtcac cgatctctct gcggcgaccg cttccgaacg agctctcaag gctgcggttg
      601 aggagaagct ctcaacaaac cgccttaccg aagggcgtgc gcaggagcag gggcttttcc
      661 cggcagcgcg gaacctcttc ggcgagaccg aaccgggaaa gactgtttct caggaaggag
      721 ttgcacagca gcaggcaggc aaacccttta ccattctcag ggagagcttg aagggcgagg
      781 atttcagtaa acaaaccggc aaagaagccg cggaatccgc cgtgtactct gcttcgccca
      841 aaccgttgca gtccgcctct cagcagcagg tttccttcgc caatcttctc tcgggagaga
      901 cgcagttgca ggggcgagga ggagcggcgc ttgaggacgg tatgcagcat gtcgttcgat
      961 tcttacgaac cgaggggcgt caggcggcca gcatcatcgt ggaccctccc gctctcggac
     1021 gtatagagat agaactggtc acaattgcga agggcgtcga ggccaccatt aaagttgcca
     1081 gcgaacaggt ccgacaactt gttcaggacc atatcactgt tctgcggaat catttggagc
     1141 agcagggggt tcatctcggc gaattcatcg tggaccttcg cgataacagc aaaggacaat
     1201 cagggcgcaa cgcgttcgcc ggcgagagaa acggccggcg gaggggagtc tccgtcttgg
     1261 acgcagaggg cattgacgaa acgatcccat ccttccggat ggatcttgag catgggctgc
     1321 tctcctgggt agcctaggag gtggaaggaa tggccataca gaacgtagcg aacagctact
     1381 ccgcaaagac gacggatacg gcgctcacag acgccatcca gaacccgagc accgatctcg
     1441 ggaaggatgc tttccttaaa atactcataa cgcagctgag caaccaggac ccgatggatc
     1501 ctttgaagga caaggatttc atcgcccaga tggcgcagtt cagcacgctt gagcagatga
     1561 ccaacatgaa caagagcatc gaacagatgg tgtccatgaa caaggcctcc gcagtcagct
     1621 acatcggcag ggtgatcgaa tatacggacg acgaggggct tcccacctac gcaacggtcg
     1681 gttacgttcg tttcgaagaa ggaaagatca ttctgaatac caccgacgga gacgtaccac
     1741 tggaaaaggt catctccgtg gcctaaagga cattttcgca ttcacggaaa tatcggaaaa
     1801 gcggccccac ggatggacgg ccttgaagtg atcggagcaa tggactgcgc cgtcttcgaa
     1861 catacagcaa ggaggaatga acatgcttcg atctcttatg tcgggcgtgt cgggcgcgag
     1921 aggacaccag acgtttctcg acgtcgtcgg caataacatc gccaacatca atacagtggg
     1981 attcaaaaaa tcgaacgtca ccttc
//
LOCUS       DAOPKU010000555         2004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_66017_length_2004_cov_4.277065, whole genome shotgun sequence.
ACCESSION   DAOPKU010000555 DAOPKU010000000
VERSION     DAOPKU010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66017_length_2004_cov_4.277065"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PK849_13475"
     CDS             complement(<1..1337)
                     /locus_tag="PK849_13475"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPE67185.1"
                     /translation="MSREQYPLERLPSVERNRGPVPFVPREGAARAPLLFILAGLSVS
                     IMAVSRGIPAPVSSVLAPLTVWGLLLILSEYRPPRFFSYGAFLSLLAFACALSCGYRL
                     VSAATIKAGVLQDEGTVVLERSWGSRRIVLVDASSGTYLMRLSPNRYILEGDALFFTG
                     RTKAIAPRPGSSFREDLYWEARGVSIEVVPESITRKAGGWSLARWRTALRKRILLTLP
                     YRTRGYLLAAVLGVRDPDLTEAHRRWGTSHLLAVSGFHVGLVAFAVWRFLSFPALRLV
                     CSRRLAAVLSSLFLWGYVLLAGGAPGALRAALMIQALLLGTVLGRKGTPLNSVSLAAL
                     LLLLWRPAWFFDIGWRLSVMAALLLAGLGERRLSLWMLPLSSLFIWFAAFPQVTATFG
                     EVPVAGIALNLVALPLFSLLYPIALFLSLPLLLSVPSGEIFAGTAEGLFSLWE"
     gene            complement(1334..>2004)
                     /locus_tag="PK849_13480"
     CDS             complement(1334..>2004)
                     /locus_tag="PK849_13480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPE67186.1"
                     /translation="FLLAGFLVYLFSGKFVNTPSGMKAGPALSLSQVPAVTESAPAVL
                     KEPPAAQKAWVLYVTGEVLSPGVYHLPPESRVFQLVEAAGGLTQRADPVQVNLAAPLD
                     DGMHIHVPAILPPAPVLERKPGDYAAPSPGKSSSAVVFPRASQPAVLRSAPQPAAGGR
                     VNVNMATAAELESLPGVGPAIARSIMECRETLGRFSCVEDLLKVKGIGAKKLEAMRGF
                     IAIQ"
BASE COUNT          533 a          597 c          589 g          285 t
ORIGIN      
        1 cactcccaga gagaaaaaag tccctcggct gtccccgcga aaatctcacc tgaaggcact
       61 gaaagcaaaa gaggaagcga aagaaaaagt gcaatcggat agagcagtga aaagagaggg
      121 agcgcgacaa gattaagcgc tattccggcg acgggaacct ctccgaatgt ggccgtcacc
      181 tgagggaacg cggcaaacca aatgaagaga ctggaaagcg gaagcatcca caatgagagg
      241 cggcgctccc cgaggcccgc aagaagaagc gcggccatca cggagagacg ccagccgata
      301 tcgaaaaacc aggcaggacg ccagagaagc agaagcagcg cagcaaggga gactgaattc
      361 aaaggagtac cctttcgtcc gagaacagtg ccaagaagga gcgcctgaat catcagtgca
      421 gcgcggagag cgcccggggc ccctccggcg agaagaacgt acccccagag gaagaggcta
      481 gaaagaacgg cggccaatcg gcgcgagcag acgagccgta gagccggaaa agaaagaaaa
      541 cgccacaccg cgaacgccac aaggccgaca tgaaaacctg aaaccgccag gagatgcgac
      601 gtcccccagc gtctatgcgc ttccgtcagg tcaggatcgc gcacgcccag aaccgccgca
      661 agcaagtacc cccgcgtccg atacggcagc gtaagaagaa tccgttttct gagcgccgtt
      721 ctccagcggg cgagcgacca tccgccggcc ttccgtgtaa tcgattcggg gacgacctcg
      781 atcgagacgc ccctcgcctc ccagtagaga tcttccctga aagacgaacc gggacgcggt
      841 gcgatcgctt tcgttctgcc tgtgaagaag agtgcgtccc cttcaagaat ataccggttc
      901 ggagagagcc tcatcaggta cgttccagag gaagcgtcca caagcacaat ccgcctgctc
      961 ccccaggacc gttccaaaac aactgttcct tcgtcctgaa gaacgcctgc ctttatggtt
     1021 gcggcgctca caagacgata gccgcaagac aacgcacaag cgaacgcgag aagcgataga
     1081 aaagcaccat aggagaagaa acggggcgga cggtattccg agagaatgag aaggagtccc
     1141 cacaccgtca gcggcgcaag aactgaagag acaggagcgg ggatcccccg cgaaacagcc
     1201 ataatcgata cagagagacc ggcgagaatg aagagcagcg gcgccctggc cgctccctca
     1261 cgagggacaa acggaaccgg gccgcgattc ctttcgacgc tgggaagccg ttccagagga
     1321 tattgttccc ggctcattgg atggcgataa accccctcat cgcttcaagc ttcttcgccc
     1381 cgatcccctt gaccttcagc agatcttcca cgcacgaaaa tcgccccaga gtctccctgc
     1441 attccataat agaacgggcg atagccggcc ctaccccggg aaggctttca agctcggctg
     1501 cagtggccat attcacgttc acccgccctc ctgcggcagg ttgcggggcg cttcgaagca
     1561 ccgccggctg ggacgcgcgc gggaacacaa cggcggatga cgactttccc ggcgagggag
     1621 cggcataatc gcccggtttt ctctccaaga ccggcgccgg aggaagaatg gcgggaacgt
     1681 gaatatgcat gccgtcatcg agaggagcag ccaagttcac ctgcacagga tcggctctct
     1741 gcgtcaatcc cccggcagct tcgacaagtt ggaacaccct gctctcaggg ggaaggtggt
     1801 agactccggg cgacagcact tcgccggtaa catagaggac ccacgccttt tgagcggcgg
     1861 ggggttcctt gagaacggca ggcgccgatt ctgttaccgc tggaacctga gaaagcgaga
     1921 gcgcaggacc ggctttcatc cccgaaggag tattcacgaa tttgccggag aagagataca
     1981 ccaggaatcc cgcgagaaga aaac
//
LOCUS       DAOPKU010000556         2003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_66076_length_2003_cov_4.222793, whole genome shotgun sequence.
ACCESSION   DAOPKU010000556 DAOPKU010000000
VERSION     DAOPKU010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66076_length_2003_cov_4.222793"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PK849_13485"
     CDS             <1..326
                     /locus_tag="PK849_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPE67187.1"
                     /translation="PEVRRAGIDAARILAPGMLVTGFQIMASGLFQALGKAGPSFVLS
                     LLRQVILLIPLVLLFSRLFGLFGVWLAFPLSDFAASLLTFAIYRREMLRLREFCETGD
                     DPTAS"
     gene            complement(313..>2003)
                     /locus_tag="PK849_13490"
     CDS             complement(313..>2003)
                     /locus_tag="PK849_13490"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPE67188.1"
                     /translation="LFVSIGYATCHWCHVMERESFDDGEVASLLNDACVPVKVDREER
                     PDVDGAFMAVCQMMNGNGGWPLNTFITPQGLPFFASTYLPKRGGGKYPGLVDVVPRVK
                     WLWKTQREQVEQSAKSIRDSLLKEEAPSSGSLPGAAQFKAAYDGLRAGYDREFGGFSK
                     EPKFPMPSHLLFLLRYWKRFGEENAWSMAESSIERIWEGGIHDHLGGGIARYATDRRW
                     LLPHFEKMLYDQALLLHVLSEYQNERGNPLFGRFADDLAGFVLREMTSPEGGFYSAFD
                     ADSEGEEGKFYLWTEDQVRAVLTPEESGIFICAYGVRKGGNVKNERTGRILGDNVLHI
                     PEPRAKTAARFALSPDELDTLLASCRTRLFAERKRRIPPLLDDKILVDWNGLMIAALA
                     RASTVFKRPEWLTAAEKAARFIETKLRDKTGKLLHRYRDKEAAIPAFLDDYAFYAWGL
                     TELAAATGKNAYTDAALKLADAMKENFLDKRNGGFFSSAGDDPMLFLRRKEAYDGAVP
                     SGNSVAMDALIRLSMISGRRDFASATKRTAEAFSERVAAFPLSHTRLLAAVMTL"
BASE COUNT          338 a          629 c          646 g          390 t
ORIGIN      
        1 cccctgaggt tcggcgcgcc ggcatcgacg ctgcgcgaat actcgcgccg ggaatgctgg
       61 tcacgggatt tcagatcatg gcgtcggggc tcttccaggc gctgggaaag gccgggccgt
      121 cgtttgttct ctccctgctg cggcaggtga tcctcctgat cccgctggtg ctgctcttct
      181 cccgcctctt cgggctcttc ggcgtctggc tcgcctttcc gctatcggat tttgcggcgt
      241 cgctgctcac gttcgccatc taccgcaggg agatgctccg cctccgcgaa ttctgcgaaa
      301 cgggcgacga ccctacagcg tcatgactgc ggcaagcagc cgcgtatgcg aaagcggaaa
      361 cgcggcgacc cgctccgaaa aggcttccgc agtcctcttg gttgcggagg caaagtccct
      421 gcgtccggaa atcatagaca gccggatcaa cgcgtccatg gccacggagt ttcccgacgg
      481 cacggcgccg tcgtaggctt cctttcggcg caggaagagc atcggatcgt cccccgccga
      541 cgagaaaaac cctccgttcc gtttgtccag gaaattttct ttcatcgcgt ccgccagctt
      601 gagcgcggcg tctgtgtacg cgttcttccc tgtggctgcg gcgagttccg tcagccccca
      661 ggcgtaaaat gcgtagtcgt ccagaaacgc cggtattgca gcctccttgt cgcggtagcg
      721 gtgcagcagc tttcccgttt tgtcgcggag ctttgtctcg atgaaccgcg cagccttctc
      781 cgccgccgtc agccattccg gccgtttgaa gacggtggag gcgcgcgcga gggcggcgat
      841 catgaggccg ttccagtcga cgaggatctt gtcgtcgagc agcggcggga tgcgacgctt
      901 ccgttcggcg aaaagccgcg tccggcacga ggcgagcagg gtgtcgagct cgtcgggtga
      961 aagggcaaag cgcgcggccg ttttcgcgcg cggttccgga atgtgcagga cgttatcgcc
     1021 gaggatgcgt ccggtgcgct cgttcttgac gttgcccccc ttccgcacgc cgtaggcgca
     1081 gatgaagatg cccgactcct cgggggtcag gacggcccgc acctggtctt cggtccagag
     1141 gtagaacttt ccctcctcac cttcgctgtc ggcgtcgaaa gctgagtaaa atcccccctc
     1201 cggagaggtc atctcacgga ggacgaatcc tgcgagatcg tcggcaaacc ggccgaacaa
     1261 cggattgccc cgctcgttct gatactccga aagcacgtgc agcagaagcg cctggtcgta
     1321 gagcatcttc tcgaagtgcg gcaacagcca tcgccgatcg gtggcgtagc gggcgatgcc
     1381 gccgccgagg tggtcgtgaa tgccgccctc ccagatacgc tcgatggagc tttcggccat
     1441 ggaccatgcg ttctcttcgc caaaacgttt ccaataacga aggaggaaga ggaggtgcga
     1501 cggcatgggg aacttcggct ctttggagaa gcctccaaac tcccggtcgt acccggcgcg
     1561 gaggccgtcg taggcggctt tgaactgggc tgctcccgga agggagcctg aagacggagc
     1621 ctcttccttc agcagggagt cgcggatgct cttcgcggac tgctccacct gttcgcgctg
     1681 ggtcttccac agccatttca ccctcgggac gacatccaca aggccggggt atttgccgcc
     1741 tccccgcttg gggaggtagg tggaggcgaa gaaggggagc ccctgcgggg taatgaaggt
     1801 attgagcggc cagccgccgt tgccgttcat catctggcag acggccatga aggcgccgtc
     1861 gacgtcgggg cgctcctcgc ggtcgacctt caccgggacg caagcgtcgt tgagaaggct
     1921 ggcaacctcg ccgtcgtcga acgactcgcg ctccatgacg tgacaccagt ggcaggtggc
     1981 gtagccgatg gagacgaaga gcg
//
LOCUS       DAOPKU010000557         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_66466_length_1996_cov_4.292117, whole genome shotgun sequence.
ACCESSION   DAOPKU010000557 DAOPKU010000000
VERSION     DAOPKU010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66466_length_1996_cov_4.292117"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..950
                     /locus_tag="PK849_13495"
     CDS             351..950
                     /locus_tag="PK849_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPE67189.1"
                     /translation="MTMNFFDAVKNRRTYYSISKKSPVSDERIREIVEQAVKHCPSAF
                     NSQSGRAVLLFGAESDAFWSLVKESLRKIVPAEKFAPTEEKIDGFASGYGTVLFFEDE
                     RPVKGLQESFPTYAEHFPVWSHHASGMLQFIVWAGLESEGFGASLQHYAPVVEQAVKQ
                     RWGFPAEWKLIAQMPFGTPTAGPGDKEFQPLEERFKVFK"
     gene            1205..>1996
                     /locus_tag="PK849_13500"
     CDS             1205..>1996
                     /locus_tag="PK849_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPE67190.1"
                     /translation="METQSGKGPHFIEQIIMDDLKNGVVKEVHTRFPPEPNGYLHIGH
                     AKSICLNFGLAEQFGGKCNLRFDDTNPAKEETEYVESIMRDVQWLGFRWHALRFASDY
                     FQQFYEWALELIHAGKAYVDHQTPDEIRETRGTLTAPGVESPYRNRSVEENLALFQQM
                     KDGVLEDGTCVLRAKIDMNHPNINMRDPVLYRILHRHHFRTGDAWCIYPMYDFAHGYE
                     DAIEGVSHSICTLEFEDHRPLYEWFLDNVSVPCTPRQFEFARLNLT"
BASE COUNT          404 a          556 c          620 g          416 t
ORIGIN      
        1 ggggaacgtg ggacggcgaa cagctgaggc gctcgcttga gaatctccct cgggaacccg
       61 cagcctgcgt taacccgcgc ggcgcgtgtg ccgccgccga tgcagtagtg cgcgctgttc
      121 ggtgacgggg tgaaatgtcc tcgttgttgt ttcaccgaag agcctttctc ttgccggctt
      181 tattttatac ggattcatgg tacaatagct tttaaagcga tgtaaattta taaaaatcat
      241 atccgaacag gcggatggtt cgccaaaata acgttctgcc gggcggatgt ggcagcaggg
      301 gaggctgaag catcctctcc gaatttgaat atatgcagga ggtatgtgca atgaccatga
      361 atttctttga cgcggtgaag aaccgcagaa cgtactacag catttcaaaa aagagtccgg
      421 tctccgacga gcggatacgc gagattgtcg agcaggcggt gaagcactgc ccctcggcgt
      481 tcaactccca gagcgggcgt gccgtgctgc ttttcggggc ggagagcgac gcgttctgga
      541 gtcttgtgaa ggagtcgctc cggaagatcg tcccggcgga gaagtttgcg ccgacggagg
      601 agaagatcga tggcttcgcc tcggggtacg gcacggtgct cttcttcgag gacgagcgcc
      661 cggtgaaggg gctccaggag agcttcccca catatgccga gcacttcccc gtctggtcgc
      721 accacgcgtc ggggatgctt cagttcatcg tctgggcggg gctggagtcg gaggggttcg
      781 gagcgtcgct gcagcactac gcgcctgttg tcgagcaggc ggtgaagcag cggtggggat
      841 tccccgccga gtggaagctg atcgcgcaga tgcccttcgg aacgccgacg gcaggccccg
      901 gcgacaagga gtttcagccg ctggaggagc gtttcaaggt attcaagtag cgtttttcaa
      961 tcgttctttc aagggtgttc cgcgattcca caaggggcgc ggaacaccct ttttccttca
     1021 tctccctctt tctacggatt tttctccgta gagggaggga gaattttaca tttcgggctc
     1081 tccgtgtgaa aataggtgcg tgcgccgcgg aaacgggcgc gctgttgcct gtatgaagca
     1141 cacgatgttt gctgatgttg cggcgaactt caatccgtta gtataaagga gcgttttctg
     1201 acctatggag actcagtccg gcaaaggccc acatttcatc gaacagatca tcatggacga
     1261 tctgaagaac ggcgtcgtca aggaagtgca cacccgcttt ccaccagagc ccaacggcta
     1321 ccttcacatc gggcacgcga agtcgatctg cctgaacttc gggctggccg agcagttcgg
     1381 cggcaagtgc aacctgcgct tcgacgacac gaacccggcg aaggaagaga cggagtacgt
     1441 cgagtccatc atgcgcgacg tccagtggct cgggtttcgc tggcacgcgc tccgcttcgc
     1501 gtcggactac tttcagcagt tttacgagtg ggcgctggaa ctgattcacg cggggaaggc
     1561 ctacgtggac catcagacgc ccgacgagat ccgggagaca cggggcacgc tgacggcccc
     1621 cggcgtcgag tccccctaca ggaaccgctc cgtcgaggag aacctcgcgc tcttccagca
     1681 gatgaaggac ggggtgcttg aggacggaac gtgcgttctc cgtgcgaaga tcgacatgaa
     1741 ccacccgaac atcaacatgc gcgatccggt gctctaccgg attctgcaca gacatcactt
     1801 ccgcaccggg gacgcgtggt gcatctaccc gatgtacgac ttcgcccacg gctacgagga
     1861 cgccatcgag ggagtctccc attcgatctg cacgctggag ttcgaggacc atcgtccgct
     1921 gtacgagtgg ttcttggaca acgtctcggt tccgtgcacg ccccgccagt tcgagttcgc
     1981 ccggctgaat ctgacg
//
LOCUS       DAOPKU010000558         1995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_66524_length_1995_cov_5.972680, whole genome shotgun sequence.
ACCESSION   DAOPKU010000558 DAOPKU010000000
VERSION     DAOPKU010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66524_length_1995_cov_5.972680"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PK849_13505"
     CDS             <1..617
                     /locus_tag="PK849_13505"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPE67191.1"
                     /translation="ELGVTRPEDEEALKKRILQAAEEGDSLGGTFVLSITGLPPGVGS
                     FAEWDERLDGLLGQAILSIPGVKGVEFGEGFHSADLPGSRLHDPIVVDGGRWTRPSNN
                     AGGLEGGMSNGGEIRLRAVMKPIPTMKKGLPSFDTVSDRPCAGHSERSDVCAVPSACV
                     VAEAMAAWTVACAVTAQFGNDRMDDLAQRFASYKQRAERWNLHA"
     gene            610..1173
                     /locus_tag="PK849_13510"
     CDS             610..1173
                     /locus_tag="PK849_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPE67192.1"
                     /translation="MHSGNICIGGFMCSGKTSVGRELSRRLGWEFLDTDVVIEQRAGM
                     PVPRIFAELGEPVFRRFEFEVVSEAVGGSNRVVSLGGGALVNTELRNRVLANACLVIL
                     DVRAETVLCRAERQKGARPLLDSGTVHSLMAKRTSAYAACHLRVATDEFPVNAVADRI
                     LANIPLTGTLTGTDTIRAAAETLCVPA"
     gene            complement(1118..>1995)
                     /gene="pheA"
                     /locus_tag="PK849_13515"
     CDS             complement(1118..>1995)
                     /gene="pheA"
                     /locus_tag="PK849_13515"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPE67193.1"
                     /translation="REIISVCRGVQHRPKVAFLGPEGSFSHEGALAAFGGAIDLLPCL
                     SFVDVFTAVEKRSADWGVVPAENSLEGTVVPTMDAFASQAGLLDSSSHNNICIHSEVS
                     IAIDHHLASPEKNLDAIREVFSHPQALGQCREWLRTNLPSAAQIPAGSTSAAAASVAG
                     APGRAAVCSALAARNNGLNILAEKIQDRGRNTTRFWTVGFGTPRKGERNKTSFLFSVE
                     HRPGSLFRAMEPLYLNGLNLTHIQSRPLSGSVFEYYFFIDVEGHIDEPQVRKALDVMR
                     ERTAFLRLLGSYPCL"
BASE COUNT          354 a          648 c          664 g          329 t
ORIGIN      
        1 cggaactcgg cgtcaccagg ccggaggacg aggaggcgct gaagaagcgc atccttcagg
       61 cagccgaaga gggcgacagc ctcggcggga ccttcgtcct ttcgatcacc ggactccccc
      121 ccggcgtcgg gagcttcgcg gagtgggacg agcggctcga cgggctgctc ggacaggcga
      181 tcctctccat ccccggcgtc aagggcgtcg agttcggcga gggcttccac agcgccgacc
      241 ttcccggcag tcgtcttcac gaccccatcg tcgtcgacgg cggacgctgg acccgcccga
      301 gcaacaacgc gggaggactc gagggcggta tgagcaacgg cggcgaaatc cgcctccgcg
      361 ccgtgatgaa gccgatcccg acgatgaaga aggggctccc ctcgttcgac actgtatcgg
      421 atcgcccctg cgccggacac tcggagcgaa gcgacgtctg cgcggttccg tctgcctgcg
      481 tcgtcgcgga ggcgatggcg gcgtggacgg tggcgtgcgc ggtgactgca cagttcggca
      541 acgacaggat ggacgacctc gcgcagcgtt tcgcatccta caaacagcga gcggaaaggt
      601 ggaatcttca tgcatagcgg gaatatctgc atcggcggct tcatgtgctc gggcaagacg
      661 agcgtcgggc gggagctttc ccgcagactc ggatgggagt tcctggatac cgacgtcgtc
      721 atcgagcagc gggcggggat gcccgtcccc agaatctttg ccgagcttgg cgagccggtc
      781 ttccggcggt tcgagttcga ggtggtctcc gaagcggtcg gcgggagcaa tcgcgtcgtg
      841 tcgctgggcg gcggcgcgct ggtgaatacg gagctgcgga acagggtgct cgcgaacgcc
      901 tgcctcgtga tcctcgacgt ccgggccgag acggtgctct gccgggcgga gcggcagaaa
      961 ggcgcgcgcc cgctgctcga cagcggcaca gtccattcgc tgatggcgaa gcggacctcc
     1021 gcctacgctg cgtgccacct ccgggtggcc acggacgagt ttcccgtgaa cgccgtggcc
     1081 gaccggattc tggcgaacat ccccctgacg gggacgctta caggcacgga tacgatccga
     1141 gcagccgcag aaacgctgtg cgttcccgca tgacgtcgag cgccttccgc acctggggct
     1201 cgtcgatgtg cccctcgacg tcgatgaaga agtagtactc gaagacgctt cccgaaaggg
     1261 gccgcgactg aatgtgcgta aggttcagcc cgttcagata gagcggctcc atggcccgga
     1321 aaaggcttcc cgggcggtgc tccacgctga acagaaacga cgtcttgttc ctctccccct
     1381 tccggggggt tccgaatccc acagtccaga aacgcgttgt gttccgcccc ctgtcctgaa
     1441 tcttctccgc gaggatattc aggccgttgt tccgtgccgc gagcgcgctg cacaccgcgg
     1501 cgcgccccgg cgccccggcg accgacgccg cagccgcgct cgtgcttccc gcggggatct
     1561 gcgccgcaga cggcaggttc gtccgcagcc actctctgca ctgcccgagg gcctgaggat
     1621 gcgaaaaaac ttcccgaatt gcgtcaagat ttttctccgg agaggcgaga tgatggtcta
     1681 ttgcaatcga aacttcactg tgtatgcaaa tattattatg agaagatgaa tccagcagcc
     1741 ccgcctgaga cgcgaacgca tccatcgtcg gaaccaccgt cccctccagc gagttctccg
     1801 ccggaaccac cccccagtcc gccgagcgct tctccacggc ggtgaagacg tcgacgaagg
     1861 agaggcaggg gagcagatcg atcgctccgc cgaaggccgc gagcgctccc tcgtgcgaga
     1921 aggagccctc ggggccgagg aaggcgacct tcgggcggtg ctgcacgcct ctgcagacgg
     1981 agatgatctc gcggt
//
LOCUS       DAOPKU010000559         1992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_66675_length_1992_cov_4.694889, whole genome shotgun sequence.
ACCESSION   DAOPKU010000559 DAOPKU010000000
VERSION     DAOPKU010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66675_length_1992_cov_4.694889"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PK849_13520"
     CDS             <1..271
                     /locus_tag="PK849_13520"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPE67194.1"
                     /translation="TVRQIGELGDEIAVTQTFLAFNAEGMYGKEYVESVVESVLSDLK
                     QAAPSEPGGEILYPGEKEGRTRRENLELGIPVNKEIWEGILRELA"
     gene            333..1331
                     /locus_tag="PK849_13525"
     CDS             333..1331
                     /locus_tag="PK849_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPE67195.1"
                     /translation="MKKCTLLFLVAVLAALFVLPAQADPLKIVAAHNQTSLENPYQHG
                     MMKFKEVLEKISGGEISVDVHAGTIGTNEDELVEKLQLGAADVVVASPGFMTKIGIPE
                     VDLFSLLYLFNSFGHWEKAVDGEAGQTLAKIINEKSHNTFRIAAYWSAGVRNYYGKKP
                     INTMDDLKGMKIRTQMSGVVADFWKQTGAIPTQVAWGELYQALQQGIVDCAENDYTNF
                     SLLDHHKTVNGKFITETEHDYTTRFVLMNGKKFDSLTDQQKAWIVEAMKEATATEREI
                     TYNMLSKSKERVIADGGTVNTIDKAPFIAIAVPIQDKLAEKLGIQNLVEMVRAAGK"
     gene            1450..1935
                     /locus_tag="PK849_13530"
     CDS             1450..1935
                     /locus_tag="PK849_13530"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE67196.1"
                     /translation="MKRLISTLVRIQEALGTLLLAIFFLAILAQISARYMAIPLLWTE
                     ELANYSFIWAVFMGASVMIHHKAHFAFTFFSDKFTGRTAAFYDAFISVILLCFTVPMA
                     YYGVIVATSFWHYNWITLPWVKMGYTWLCLPITGCTMTIYTVAHIIDDIRILSSKEAV
                     S"
BASE COUNT          427 a          626 c          553 g          386 t
ORIGIN      
        1 caccgtccgt caaatcggcg aactcggcga cgagatcgcg gtcacccaga ccttcctcgc
       61 cttcaacgcg gagggaatgt acgggaagga gtacgtggaa tccgtcgtgg aaagcgtcct
      121 ctccgacctg aaacaagcgg ctccgtcgga accggggggc gaaatcctct accccggcga
      181 gaaggagggg cgcacccgca gggagaacct cgaactgggc atccccgtca acaaagagat
      241 ctgggagggt atcctccggg agctcgctta aagttcgtgc tttcgcggaa gttttttccg
      301 catatcttaa ccaagagaaa ggggtaccaa aaatgaagaa gtgcacactg ttgttccttg
      361 tagctgttct tgcggcgctc tttgtccttc ccgcgcaggc cgatccgctg aagatcgtgg
      421 cggcgcacaa ccagacgtcc cttgagaatc cgtaccagca cggcatgatg aagttcaagg
      481 aagtgcttga gaagatctcc ggcggcgaga tctccgtcga cgtccacgcc ggaaccatcg
      541 gcaccaacga ggacgagctc gtcgagaagc tccagctcgg cgcggccgac gtcgtcgtgg
      601 cctcccccgg cttcatgacg aagatcggca tccccgaggt cgacctcttc tcgcttctct
      661 acctcttcaa cagcttcggg cactgggaga aggccgtcga cggcgaagcc ggacagacgc
      721 tggcgaagat catcaacgag aagtcgcaca acacgttccg tatcgccgcc tactggtccg
      781 ccggagtccg caactactac ggcaagaaac ccatcaacac gatggacgac ctcaagggca
      841 tgaagatccg cacccagatg tccggcgtcg tggccgactt ctggaagcag accggcgcca
      901 ttccgacgca ggtcgcctgg ggcgagctct accaggcgct gcagcagggc atcgtcgact
      961 gcgccgagaa cgactacaca aacttcagcc ttcttgatca ccataagacg gtcaacggca
     1021 agttcatcac cgagaccgag cacgactaca ccacccgctt cgtgctgatg aacggcaaga
     1081 agttcgactc ccttaccgac cagcagaagg cgtggatcgt cgaggccatg aaggaagcca
     1141 ccgcgacgga gcgcgagatc acctacaaca tgctctccaa gtcgaaggag cgcgtcatcg
     1201 ccgacggcgg cacggtgaac acgatcgaca aggcgccctt catcgccatc gccgtgccca
     1261 ttcaggacaa gctcgccgag aagctgggca tccagaatct cgtcgagatg gtccgcgctg
     1321 cgggtaaata agcttttttc tcctcctgcg ggaggtcagc ggacattccc cgtgaaaccc
     1381 gggcggggaa tgtccctttt tcttcgcccc gaaaggcgac ggacgaggtt ttcaaagagg
     1441 aggagaaccg tgaaaagact gatttcgact cttgtgcgca ttcaggaggc cctgggaacg
     1501 ctgctgctcg ccatcttctt cctggccatc cttgcacaga tttccgcccg ctacatggcg
     1561 atcccgctgc tctggacgga ggagctggcc aattactcct tcatctgggc agtctttatg
     1621 ggagcgtccg tgatgatcca ccacaaggcg catttcgcct tcaccttctt cagcgataaa
     1681 ttcaccggga ggacggcggc gttctacgac gccttcatct ccgtcatcct gctctgcttc
     1741 accgttccca tggcatacta cggcgtgatc gtcgccacgt cgttctggca ctacaactgg
     1801 atcacgctcc catgggtcaa gatggggtac acatggctct gccttcccat caccggatgc
     1861 acgatgacga tctacactgt cgcgcacatc atcgacgaca ttcgtattct ctcctcgaag
     1921 gaggccgtgt catgacgccg gcgctgatcg tcgggttttt cgtgctgctc atcttcctcg
     1981 gcgtccccat cg
//
LOCUS       DAOPKU010000560         1992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_66690_length_1992_cov_3.616933, whole genome shotgun sequence.
ACCESSION   DAOPKU010000560 DAOPKU010000000
VERSION     DAOPKU010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66690_length_1992_cov_3.616933"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /gene="alaS"
                     /locus_tag="PK849_13535"
     CDS             complement(<1..1109)
                     /gene="alaS"
                     /locus_tag="PK849_13535"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPE67197.1"
                     /translation="MQWRSGREIRSLFLDFWVSKGSRHVPSFSLIPDDPSLLFTIAGM
                     VPFKPYYLGIQTPESPTAVTSQKCVRTNDIENVGRTARHHTFFEMLGNFAWGDYFKKE
                     AITWAWEFLTEVIGLEPERLSATIYKDDEEAYDVWHNIVGLPDERIFRFDKDANFWFM
                     GTQGPCGPCSEIMYDQGPEFSCGRPECAVGCDCDRYLEIWNLVFTQFDLQKDGSLVPL
                     PKKNIDTGMGLERLTSVVQRVRTDFETDLFRPMIDRACSLGGIQYGYSKKTDLAVRVI
                     ADHIRSVVFMIADGILPSNDGRGYVLRRLLRRAARFGRLLGVDRPFLLDFVPDVIALM
                     GDPYREIVDNRLMVEQIIDVEEKRFGRTLQQGTELL"
     gene            complement(1239..>1992)
                     /locus_tag="PK849_13540"
     CDS             complement(1239..>1992)
                     /locus_tag="PK849_13540"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE67198.1"
                     /translation="HAPYTVPLPLMQEICAAAAERAMGVHFHFLETEWELSYLRDELK
                     MTPKEYLESSGILAAPYGILAHCVWLDAETVGAVDFSRMAIAHNPNSNLKLGSGVMDL
                     TGLGGKTKKIALGTDGVASNNRLDIWGEMRCAALLHKGIRRDPMALSACDALRMATFE
                     GASALGFKKKGLLKEGWVADLVLVDLDQPHYIGIGTENAAPFLVYAGSSADVYATMVN
                     GAWVYRDRVFPGMDSEKILAKAGEMRTDLLGR"
BASE COUNT          388 a          671 c          560 g          373 t
ORIGIN      
        1 agaagctcgg taccctgttg cagcgtccgg ccgaagcgtt tttcttccac gtcgatgatc
       61 tgctcgacca tcaggcggtt gtccactatc tcacggtagg gatcgcccat cagcgcgatc
      121 acgtcgggga cgaagtcgag caggaagggg cggtcgacgc cgagcagacg gccgaagcgc
      181 gccgcacgtc gaagcagccg gcggagcacg tacccccgcc cgtcgttcga cggcaggatg
      241 ccgtccgcga tcatgaagac aactgaacgg atgtggtccg cgatgacgcg cacggcgagg
      301 tcggtcttct tgctgtagcc gtactgaatg ccgcccaggc tgcacgcccg gtcgatcatc
      361 ggccggaaga gatccgtctc gaagtcggtg cgcacccgct gcacaaccga agtcagacgc
      421 tcaagcccca taccggtgtc gatattcttc ttcggcaacg gaaccaaaga gccgtccttc
      481 tgtaggtcga actgcgtaaa caccagattc cagatttcga ggtacctgtc gcagtcgcac
      541 cccacggcgc actcaggccg tccgcacgaa aactcgggcc cctggtcgta catgatctcg
      601 gaacacggac cgcacggccc ctgcgttccc ataaaccaga agttcgcgtc cttgtcgaag
      661 cggaagatgc gctcgtcggg aagaccgacg atattgtgcc aaacgtcgta cgcttcttcg
      721 tcgtccttgt agatcgtcgc ggagaggcgt tccggctcca ggccgatcac ttccgtcagg
      781 aactcccacg cccaggtgat cgcctccttc ttgaagtagt ccccccacgc gaagttcccc
      841 agcatctcga agaaggtatg atggcgcgcc gtccgtccga cgttctcgat gtcgtttgta
      901 cgcacgcact tctgcgacgt caccgccgtg ggcgactccg gcgtctgaat ccccagatag
      961 tacggcttga aggggaccat ccccgcgatg gtgaaaagca gcgaaggatc gtcgggaatg
     1021 agcgagaagc ttggaacatg ccggctcccc ttcgacaccc aaaaatccag aaacaaagaa
     1081 cgtatctccc tacccgatct ccactgcata tacactcttc ctccccgtag cccccggaat
     1141 catttccgtg agcggtagta tcttcctccc cgctcggggg agttcttggc cgggaacgcc
     1201 cgatcggccc cggcaacctt cagcgtgcca gattcccctt atctgcccag aaggtctgtc
     1261 ctcatctccc cggcctttgc caggattttc tccgaatcca taccggggaa taccctgtcg
     1321 cggtagaccc aagcgccgtt caccatcgtc gcgtacacat cggccgagga tccggcgtac
     1381 acaaggaagg gggcggcgtt ctccgtcccg atgccgatat agtgcggctg atcgaggtcg
     1441 acgagaacaa ggtcggcaac ccagccttcc ttgagcagtc ccttcttctt gaagccgagg
     1501 gcggaagcgc cttcgaacgt ggccatccgg agcgcgtcgc acgcggaaag cgccatggga
     1561 tcccggcgga ttcccttgtg cagcagcgcg gcgcagcgca tctcgcccca gatgtccagg
     1621 cggttgttgc tcgccacccc gtccgttcct aaagcaatct tcttcgtctt cccgccgagg
     1681 ccggtgagat ccatcacgcc gctccccagc ttcaagttgc tgttggggtt atgggcgatc
     1741 gccattctgg aaaaatcaac ggcgcccaca gtctccgcat ccagccagac gcagtgcgcc
     1801 agaatcccgt acggagccgc cagaatcccg gaggactcca ggtactcctt cggcgtcatc
     1861 ttcagctcgt cccggaggta cgagagctcc cactccgtct ccaaaaagtg gaagtgcacc
     1921 cccatcgcgc gctccgccgc cgccgcgcag atctcctgca tcagcggcag cggcacggtg
     1981 tacggcgcgt gc
//
LOCUS       DAOPKU010000561         1991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_66749_length_1991_cov_3.102273, whole genome shotgun sequence.
ACCESSION   DAOPKU010000561 DAOPKU010000000
VERSION     DAOPKU010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66749_length_1991_cov_3.102273"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PK849_13545"
     CDS             <1..347
                     /locus_tag="PK849_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67199.1"
                     /translation="GLLSAVAGMLTAGGQDSVSNTMGNGMTLLAFAGALLGGTSMSGG
                     KGSAFGMLGGAILLGMFSNALNLLGVKVTLIHAAQGALIFVAILTDRFRISLRARLLR
                     NEQLQKLKVAKG"
     gene            415..1473
                     /locus_tag="PK849_13550"
     CDS             415..1473
                     /locus_tag="PK849_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPE67200.1"
                     /translation="MNSSFRKVIPALLALFVFSAASCASEKQISIGMIIKEPSAPYIQ
                     AFIKAAEETAASLGVNIMIRDGEADSIKIMEIIDTFMIQGIDAFILGGAVDLRTLVPG
                     IVRLNEAGIPVAALDTSPEGGVVDFFLSFDLAQSSARAAELFVEGIKSRNSGDIPEGV
                     VLEILGDKADMFSHACTEGFNSVLSKYPQLEIAQGEGKWNNTDSHAVTSDLLTRFGKR
                     VVGIYVQTPDIMGPGVVAAIEAAGLDSADYGICGICIGPEGIDLIKKGKMLGAVAQPA
                     YDSAALAVRFLVDKLRGNPVPRIGDTLTEDGALWSPAKVIKNPWAEDGAFIVLQGPLV
                     PSEVSPDDPRLWENMLTK"
     gene            1531..>1991
                     /locus_tag="PK849_13555"
     CDS             1531..>1991
                     /locus_tag="PK849_13555"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE67201.1"
                     /translation="MYGKKRVSSVFCVLLVLFLFSIAGQAAAGARLDRVMETKLLRVG
                     TPGDYRPFAMLDKSTGKYEGHDIELAELLASELGVNVVFVPTTWSKLMEDYLAGKFDV
                     AVGGITRSLARMVKGDFLPPYAPNGKVAIIRKADKEKFTSLEAMDVPETTV"
BASE COUNT          461 a          490 c          573 g          467 t
ORIGIN      
        1 ccgggcttct ctccgctgtt gccggtatgc tgacagccgg ggggcaagat tcggtgtcca
       61 acaccatggg gaacggaatg acgctccttg cattcgccgg agcgcttctc ggaggcacct
      121 ccatgagcgg agggaaaggc tccgctttcg ggatgctcgg aggtgcgatc cttttgggca
      181 tgttttcgaa tgctcttaat cttctcggtg tcaaggtgac gctcatacac gccgctcagg
      241 gggcgttgat atttgtcgcc atacttacag accggtttcg aatctccctc cgggcacgat
      301 tgcttcgtaa cgaacagctc caaaaactca aagtcgcgaa aggatgatgg caataattga
      361 gaatgaggaa agattttaaa gtacgttcat ccactacatc gaggaggaat ctacgtgaac
      421 agctctttta gaaaagtaat accggcgctt ctggcgcttt tcgttttttc tgcggcttca
      481 tgtgcgtccg aaaagcaaat ctccatcggt atgatcatca aagaaccctc ggcgccatac
      541 attcaggcat ttataaaggc agcggaagag acagccgcct cgctgggcgt gaatataatg
      601 attcgcgacg gcgaagccga ttctataaaa attatggaaa tcatcgatac ttttatgata
      661 cagggaatcg acgcgttcat ccttggcggc gctgtggatt tgcgcacgct ggtgcctggt
      721 attgtgcgcc tcaacgaggc tgggatcccc gtggccgcac tggacacatc tcctgaagga
      781 ggcgttgtcg atttcttcct ctcctttgac ctcgctcagt cgagcgcccg cgccgcggag
      841 cttttcgtcg aggggatcaa gtccagaaac agcggcgata tacccgaggg cgtcgttctt
      901 gagatactcg gcgacaaagc ggatatgttc agccatgcct gcaccgaggg attcaactcc
      961 gtgctgtcga agtatccgca gcttgaaatt gctcaaggag agggaaaatg gaataatacc
     1021 gactcccacg cagtgacttc cgatttgttg acgcgtttcg ggaaacgggt tgtgggcatt
     1081 tatgtccaaa cccctgatat aatgggaccc ggtgtcgttg cggctatcga ggccgcaggg
     1141 ttggattccg ccgactacgg tatctgcgga atatgcatcg gccccgaggg aatcgacctc
     1201 attaaaaaag ggaagatgct cggcgctgtc gcacagcctg cctacgactc ggccgcgctg
     1261 gcggtgaggt tccttgtgga caagctgaga gggaatcctg ttccacggat aggagatacg
     1321 ctgacggaag acggcgctct ctggtctccc gcaaaagtca taaagaaccc ttgggccgaa
     1381 gacggagcct ttatcgttct ccaaggaccg cttgttcctt cagaagtctc ccctgacgac
     1441 ccccgtctgt gggagaatat gctgacgaag taaacgggtt ttgagatgag agagaatgtt
     1501 acgaaacaaa aaacgaacgg aggggtattg atgtacggaa aaaagagagt ttcgagtgta
     1561 ttttgtgtac tgctggtgct gtttcttttc tcgattgcgg gacaggctgc agcaggagcg
     1621 aggcttgacc gcgttatgga aacaaagcta ttgcgggtgg ggactcccgg ggattatcgc
     1681 ccattcgcca tgctcgacaa gagtacggga aaatacgagg ggcacgatat cgaacttgcg
     1741 gaacttcttg cgtcggagct tggcgtgaac gttgtatttg ttcccaccac ctggtcgaag
     1801 cttatggagg attaccttgc aggcaaattc gacgtcgccg tcggaggtat aacccggagc
     1861 cttgcccgta tggtgaaggg ggactttttg cctccgtatg caccaaacgg caaggttgcc
     1921 atcattcgga aggctgacaa ggagaagttc acttcgcttg aagcgatgga cgttcccgag
     1981 acgacagtca t
//
LOCUS       DAOPKU010000562         1990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_66775_length_1990_cov_3.605685, whole genome shotgun sequence.
ACCESSION   DAOPKU010000562 DAOPKU010000000
VERSION     DAOPKU010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66775_length_1990_cov_3.605685"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK849_13560"
     CDS             <1..186
                     /locus_tag="PK849_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67202.1"
                     /translation="SGEPPDLRAGRVLNRLFHTRLNGEFRDGLKMLEKEAGGAESTGL
                     RAARSSSGRRAALDMPR"
     gene            complement(585..935)
                     /locus_tag="PK849_13565"
     CDS             complement(585..935)
                     /locus_tag="PK849_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67203.1"
                     /translation="MIVCAAPLQNVPQRGEHRGGGERERLSHSDLVRKQQARHSVVLP
                     VGEKRRYERALSRLKTFASAIDRRFGKGSPGQFAGSGLLEADFNAIRYTANLFDDGVG
                     KRVGVGPENLELLL"
     gene            950..1384
                     /locus_tag="PK849_13570"
     CDS             950..1384
                     /locus_tag="PK849_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67204.1"
                     /translation="MKTLILQRICSSFASGRLTAERMLRKEALEDEDEEKQSIEGTLR
                     GMTEEERHYLESIVEELSRPEAHDPKVAAVRYFLSEHRTDGKNWLEHGCIIFSQYYDT
                     VRTLALELGSVLPGEPVAVYAGAGKSGIHLDGDFASVEREEI"
     gene            1391..1870
                     /locus_tag="PK849_13575"
     CDS             1391..1870
                     /locus_tag="PK849_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPE67205.1"
                     /translation="MRKHTIRLLVATDAACEGLNLQAIGTLINVDLPWNPSRLEQRLG
                     RIKRFGQRRRSVDMLNLVYHDTQDEKVYAALSSRMKDRYDIFGSLPDTIEEEWIESVE
                     ELEKMMDRYIHLRKKAKDAFEIRYERSVNPESERWELCSRVFARRAIVDRLSKPWKG"
BASE COUNT          437 a          505 c          624 g          424 t
ORIGIN      
        1 agcggtgaac cgcctgatct tcgagcggga cgcgttctga acagactgtt tcatacgagg
       61 ctgaacggag agttccgtga tggtttgaaa atgctagaga aggaagcggg cggcgcggag
      121 agcacaggtc tccgtgccgc ccgttcttcg tcggggcggc gggcggcgct ggatatgccc
      181 cgataggcag ataaaagatg atgaagagct tgactaatat tgatctttag agaggctgtt
      241 catttccgta gcagcatcca ggacggaaca aacgcttttg cccggcgcgc cgtggagccg
      301 gggaacggag atgggaactc tgttcgcctg tgtttgtccg caggggcagg gtggacggac
      361 ttcggtttcg gctatcggcg attggaggga agcacaagtc ttccctccaa ttgaagtagc
      421 tgttcgcagc ttgacaacat ataccctatt gactattgtt agcttgcttt accttatttt
      481 attccaatgt aaacaacacg tgctggacgg gatggcgggt gactcgccgg gaaacggtcg
      541 tcccctccgc cggttcccaa aagtacccta taccgtgatg aagcctagag gaggagttcg
      601 aggttctctg gtccgacgcc tacccttttt ccgacgccat cgtcgaagag gttcgccgta
      661 tagcggatcg cgttgaaatc cgcttcgagg aggccgcttc cggcgaactg gccggggctg
      721 cccttgccga atcgccgatc tatcgcggag gcaaatgtct tcagccgtga cagcgctctt
      781 tcgtaacgac gtttctcgcc caccggcaaa actacggaat ggcgcgcctg ctgcttgcgg
      841 acgaggtcgg agtgggaaag acgctctcgc tcgccgccgc cgcgatgctc tccgcgctgc
      901 ggaacatttt gtagaggcgc tgcgcagacg atcaaaagcc gctttcttca tgaagacgct
      961 gattttacag cgtatctgct cgagtttcgc ctcggggcgt ttgaccgccg aaaggatgct
     1021 gcgaaaagaa gcgctcgaag atgaggacga agagaagcag agtatcgagg gaactcttcg
     1081 cggaatgacg gaagaagaga ggcattatct cgagtcgatt gtcgaggaac tctcccgtcc
     1141 cgaggcgcac gatccgaagg tcgccgctgt ccgctacttc ctctccgaac acagaacaga
     1201 cggcaaaaac tggttggagc acggctgtat catcttcagc caatactacg ataccgtgcg
     1261 tacgcttgca ttggagctgg gctccgtctt gcccggagaa ccggtcgcgg tgtacgccgg
     1321 agcgggcaag agcggtatcc atctcgacgg cgattttgcc tcggtggaac gcgaggagat
     1381 ctagtccgct gtgcgaaagc atacaattcg tctgcttgtt gcgaccgacg ctgcttgtga
     1441 agggctgaat ctacaggcga tcgggacgtt gatcaatgtc gatcttccgt ggaatccttc
     1501 tcgcctggag cagcgtctgg gacgaatcaa gcgatttggt cagcggcgac ggtcggtgga
     1561 tatgctcaac ctcgtctacc atgataccca ggacgagaag gtgtatgccg ccctctccag
     1621 ccggatgaag gaccggtacg atattttcgg cagcctgccg gataccatag aggaagagtg
     1681 gatcgaatcc gtggaggagt tggagaagat gatggaccgg tatatccacc ttcggaagaa
     1741 ggcgaaggac gctttcgaaa tccgttacga acgctcggtg aatccggaga gcgaacgatg
     1801 ggaactctgt tcgcgggtgt ttgcccgcag ggccatcgtg gacaggcttt ccaagccgtg
     1861 gaaggggtag cgcacacgat tcatcggatg cgttcataat cgtagtcgga aggaatggtg
     1921 actcgcatga agaagagcga gctgtgcaaa ctgctggagg ttggcgagtg gaaagacgtc
     1981 gagttcaagg
//
LOCUS       DAOPKU010000563         1981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_67220_length_1981_cov_5.153167, whole genome shotgun sequence.
ACCESSION   DAOPKU010000563 DAOPKU010000000
VERSION     DAOPKU010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67220_length_1981_cov_5.153167"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PK849_13580"
     CDS             complement(<1..453)
                     /locus_tag="PK849_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67206.1"
                     /translation="MSLLRIPVLLVLFLLFVPALPALSAPSDLIVGVEDGWIWQVALL
                     PPEDGWESDAGRSALGAVRYAERKVQESPDGVSGRDIRFHQEESLDEASAAARVEDWR
                     KRGISAVLSFAGGREIAFLRPHLGEAGPVFLSAFGEAANIYNGEGVPDT"
     gene            complement(552..1049)
                     /locus_tag="PK849_13585"
     CDS             complement(552..1049)
                     /locus_tag="PK849_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HPE67207.1"
                     /translation="MPEIKKWELVQLDFPSDCNIILGQSHFIKTVEDLFEALATSSPA
                     LQFGIAFCEASGDCLIRFDGNNEELVKIAVDNMNSLGAGHSFLIVLKNGYPINVLNRI
                     KECQEVCRVFAATANPIQVIICESEQGRGVAGVIDGFSPKGVETESDIEGRKTLLRRI
                     IGYKR"
     gene            complement(1096..1926)
                     /locus_tag="PK849_13590"
     CDS             complement(1096..1926)
                     /locus_tag="PK849_13590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPE67208.1"
                     /translation="MRMKLLVTDIDETLSRGEQLTPGVISACERLRGNGWTIMVASGR
                     ILATAMPHIRGTGTDLPAILYDGGRIMDTSTGRAVSETSMDPALALEIVQAGWEHPVE
                     LQIIGDERAVCRSADAVTRGFFAEAGVPVDSSLESPSVPQEVFRVIFHGIPGSIRSLE
                     RDLKSRFAGRAEIVQAGEGFLDVLPLGVSKGAALRGWLATLPELPEIVVAAGDHHNDM
                     EMLKEADIAVVPEDAADEVLALSDVIMPSADKDGFVFLADWLLKGDIHRDENASPVVL
                     "
BASE COUNT          411 a          635 c          540 g          395 t
ORIGIN      
        1 tgtatcgggg acgccttcac cgttgtagat gttcgcagct tctccaaaag cagagagaaa
       61 cacggggccc gcttctccga gatgcggccg gagaaaggcg atttctctcc ctccggcaaa
      121 ggaaagcact gcggagattc cgcgcttccg ccagtcctca acccgcgccg cggcggaagc
      181 ttcgtcaagc gattcctcct ggtggaagcg aatgtcgcgg ccgctcactc cgtccggact
      241 ctcctgcacc ttccgttcgg cgtaccgtac ggctccgagc gcggaacgcc ccgcgtcgct
      301 ctcccacccg tcctccggcg ggagaagcgc aacctgccat atccacccgt cctccacgcc
      361 gacgatcagg tcgctgggtg cggaaagggc gggaagcgca ggtacgaaca gcaaaaagag
      421 caccaaaaga acagggatgc gcagaaggct cacggagaga ggagaacgac cggtcccgca
      481 tacaggcctt ctcttcgtca ggcccttccg accgtccgag gcgcacgctc tccgagattg
      541 atgcgcgacc attaccgttt gtagccgatt atccggcgaa gcagtgtttt tctcccctca
      601 atgtcgcttt cggtctccac gcccttggga gagaagccgt cgatgacgcc cgccacgcct
      661 ctcccctgtt cgctttcaca gatgatgacc tgaatgggat tggccgtggc cgcaaagacg
      721 cggcagactt cctgacactc cttgatcctg ttgaggacat tgatcgggta cccgttcttg
      781 aggacgatga gaaaggaatg tcccgctccc agggagttca tgttgtccac ggctattttg
      841 acgagctctt cattgttgcc gtcgaaccgt atgaggcagt ctccggaggc ttcgcagaaa
      901 gcgataccga actgcagcgc cggagaggag gtcgcaaggg cttcgaacag gtcctcgacg
      961 gtcttgataa aatggctctg tccgagaata atattgcagt cgctcggaaa atcaagctgc
     1021 acaagctccc acttcttaat ttcaggcatg aattgtccct ccctcttctt ttcaggggta
     1081 attgtagcgg cctatctata atacaacagg agatgcgttt tcgtcacgat gaatatctcc
     1141 cttgaggagc cagtccgcga ggaagacaaa gccgtccttg tccgccgacg gcatgatcac
     1201 atccgaaagc gcaagcacct cgtccgccgc gtcctccggc acgacggcga tatcggcctc
     1261 tttcagcatc tccatgtcgt tatggtgatc ccctgccgcg accacgatct ccggaagctc
     1321 cggcagcgtc gcgagccatc cgcgaagggc cgctccctta gaaaccccga gaggaagaac
     1381 atcgagaaac ccctcgcccg cttgcactat ctccgctctt ccggcgaagc ggctcttcag
     1441 atcccgctcg agcgacctta tgctcccggg aatgccgtga aagatgaccc tgaatacctc
     1501 ctgagggacg gagggcgatt caagcgagga atcgacggga acgcccgcct ctgcgaaaaa
     1561 gccccgggtc accgcgtcgg cgcttcggca gacggcacgt tcgtcgccga tgatctgaag
     1621 ctccacgggg tgctcccacc ccgcctgcac tatctccagc gccagcgccg gatccatgga
     1681 ggtctcggaa acagctctcc cggtggaagt gtccatgatc cgccccccgt cgtagaggat
     1741 cgccggaaga tccgtccccg ttccccggat atgcggcatc gcggttgcca gaattctccc
     1801 cgaagctacc atgatcgtcc atccgtttcc ccggagacgc tcgcaggcgg aaatcacacc
     1861 gggcgtgagc tgctccccgc gggaaagcgt ctcgtctata tcagttacaa gaagtttcat
     1921 tctcatctct ttttcctccg aagaacagta gtaaaaaagg gagccgcttg cttgtatctc
     1981 c
//
LOCUS       DAOPKU010000564         1978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_67376_length_1978_cov_4.359334, whole genome shotgun sequence.
ACCESSION   DAOPKU010000564 DAOPKU010000000
VERSION     DAOPKU010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67376_length_1978_cov_4.359334"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..440)
                     /locus_tag="PK849_13595"
     CDS             complement(3..440)
                     /locus_tag="PK849_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67209.1"
                     /translation="MFEGKRKKIALWVLLLLLFTGGVSRAFDLGDLVGVIGGGFVVST
                     IAGPINDFINTITLNKGVKVEGHTKVVPIVSIGSGTHIGAAQISGPRGEPVGTTKAVA
                     QIEATFRDRLRIKILVPVDSENPVQRFRRVQGVGVSAIIDFRL"
     gene            complement(458..1477)
                     /locus_tag="PK849_13600"
     CDS             complement(458..1477)
                     /locus_tag="PK849_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67210.1"
                     /translation="MRRTILVLVCVVLLLMGAVLPLFAQEDSSTFHVMSRVEQLVYGG
                     ERTGGLVDRLNSVERDMFGRELPGSIAERQTALLTFIEKGTEYQPSLLFKLGVAEWAV
                     ERQIDSLSPVVKRVDALEVQLEGTPQVERPVTMRLERVLGLLLSEPVTAAEVELPAAT
                     IIRASLAHSLSPKTAKKGDPVDMVLHSDLVVGANLIAPKGSRVSAVVTEVTKPRSFGR
                     PGEVKVGVESLFSIGSQPIPLTFGEESRQAVEAESAQLAAAGTSVVGALLLGPVGLVG
                     GFLVRGDLKELPAGTVVFSQTADAVKVGAYPVPPGLQGLLKNPEKAEPAQTGTSGSAG
                     NETTP"
     gene            complement(1492..>1978)
                     /locus_tag="PK849_13605"
     CDS             complement(1492..>1978)
                     /locus_tag="PK849_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556273.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPE67211.1"
                     /translation="IPYFGLCLGMQVAVVEFARHVCSLPGAHSSEMDPGCANPVIHLM
                     EEQMRVEDMGGTMRLGTYPCELVPGTKTASAYGVARVNERHRHRYEFNNDYRKRLEEK
                     GMVIAGVYEEKNLVEIVELQDHPWFVGVQFHPEFRSRPVKPHPVFMGFIGAAVQRSQS
                     D"
BASE COUNT          427 a          642 c          493 g          416 t
ORIGIN      
        1 acctatagtc taaaatcaat gatcgccgaa actccgaccc cctgaacccg ccggaaccgc
       61 tgcaccggat tctcagaatc caccggcacc agtatcttta tacgcagacg atctctgaag
      121 gttgcctcta tctgcgccac agcctttgtc gttcccacgg gttctcccct cggcccggaa
      181 atctgcgccg cgccgatatg cgttccggag ccaatggaaa caataggaac gacctttgta
      241 tgcccttcca ccttgacgcc cttgttcaac gtaattgtat tgatgaagtc gttgatcggc
      301 ccggcaatgg tcgagacgac aaaacctcct ccgatgacgc cgacgagatc gccgagatcg
      361 aacgcacgtg aaacgccgcc cgtaaacagg agcagcagaa gaacccaaag cgcgattttc
      421 tttctttttc cctcgaacat gacacgtttc ctccttctca aggagtggtt tcgttaccgg
      481 cggaaccgct cgttccagtc tgtgcaggct cggctttctc cgggtttttc aacagcccct
      541 gcaatcccgg cggcaccggg tatgccccca cttttaccgc gtcggcagtt tgcgaaaaga
      601 caacggttcc ggcgggaagc tccttcaagt cgccgcgaac gagaaaaccg ccgaccagcc
      661 ctacaggacc caggagcaat gcgcccacca cactcgtgcc tgcggcggca agctgcgccg
      721 attccgcttc gaccgcctgc ctcgactctt cgccgaatgt cagcggaatt ggttgggaac
      781 cgatcgaaaa caacgactct actccaacct ttacttctcc gggacgtccg aagctccggg
      841 gttttgtcac ctccgtcact accgcagaga ctcggctgcc cttcggagcg atcaggtttg
      901 ccccgacgac caggtcggaa tggagcacca tgtcgaccgg atcacccttt ttggccgtct
      961 tcggcgagag cgagtgcgcc agagacgccc gaataatcgt cgctgcgggg agttcaactt
     1021 ccgcggctgt caccggttcg gagagaagaa gtccgaggac ccgttcgagt ctcatcgtga
     1081 ccggccgttc cacctgcggc gttccttcga gctggacttc aagagcatcc actcttttta
     1141 caacaggaga gagggagtca atttgccgct cgaccgccca ttccgccacg ccaagcttga
     1201 aaagaagcga cggctggtac tcggttccct tctcgataaa cgtcagcagg gctgtctgcc
     1261 gttcggcgat acttccgggc aactcccttc cgaacatatc tctttccacg gaattcaggc
     1321 ggtcgaccag tccgccggtg cgttcgccgc cgtacacaag ctgctccaca cggctcataa
     1381 catgaaaagt cgaagaatct tcctgggcga acaacggtaa taccgcgccc atcagaagca
     1441 gtacaacgca tacaagaacg agtatagtac gtctcattcg tttcccctcc ttcaatctga
     1501 ctgactccgc tgcaccgctg caccgataaa gcccatgaac acggggtgcg gctttacggg
     1561 gcgggagcgg aactccgggt ggaactggac gccgacaaac catggatgat cctgcaactc
     1621 gacgatctcc acgaggtttt tctcctcgta gacgcccgcg atcaccatcc ccttctcctc
     1681 aagacgcttt ctgtagtcgt tgttgaattc gtatctatgg cgatggcgct cgttcacacg
     1741 ggcaaccccg tatgcggacg ctgtcttcgt tcccggaaca agctcgcacg gatacgttcc
     1801 caaacgcatt gtcccgccca tatcctctac tcgcatctgc tcttccatca agtgtatcac
     1861 agggttggcg catccggggt ccatctccga actgtgcgcc ccgggaagac tgcatacatg
     1921 gcgtgcaaac tccaccaccg caacctgcat tcccaggcaa aggccgaaat agggaatt
//
LOCUS       DAOPKU010000565         1977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_67425_length_1977_cov_3.937565, whole genome shotgun sequence.
ACCESSION   DAOPKU010000565 DAOPKU010000000
VERSION     DAOPKU010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67425_length_1977_cov_3.937565"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PK849_13610"
     CDS             complement(<1..306)
                     /locus_tag="PK849_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67212.1"
                     /translation="MRIYKTLAAAAGMGLLFAWMESVFLQYGLLQRLAAIEYEGMNAV
                     VIFLAVHALTYLFAAFFAPGEKGERPRCAIAACLMLSLPLLIGLAQYLPASLSVVAAL
                     "
     gene            461..1015
                     /locus_tag="PK849_13615"
     CDS             461..1015
                     /locus_tag="PK849_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPE67213.1"
                     /translation="MSVKNAMTAEFLRSAYGGESMAHMRYLGWADKADKEGFPKIGRL
                     FRAVAYAEQVHATNHFVVLNGDVADTALTAGAVFGMKETAENLVGGIMGEEHEVEQMY
                     PVYYEAAKFQGEKDAMRTFNFALQAEKQHAELFRAARECALAKKDMEFEAVYVCPVCG
                     HTVTDEAPEKCPVCGVAKEKYARF"
     gene            1102..1785
                     /gene="sdaAB"
                     /locus_tag="PK849_13620"
     CDS             1102..1785
                     /gene="sdaAB"
                     /locus_tag="PK849_13620"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HPE67214.1"
                     /translation="MSFLDIVGPVMIGPSSSHTAGAARLGRLAKACWGTDTLNEVTVF
                     LRGSFAFTSKGHGTDKAVVAGLMGMLPDDERIRNALMLAKEAHFPYRLTTEDVDGAHP
                     NSARFSFLGKNGRRLDVVGASIGGGAVEIQEIDGFSVAINGELPTLITFHKDTRGVVA
                     AVAALMAGREINIASMTLHRKTRGGVATLVTELDMNAPPDEQLCNDVLQAHPAILRVA
                     SLFGGGNNS"
     gene            1782..>1977
                     /locus_tag="PK849_13625"
     CDS             1782..>1977
                     /locus_tag="PK849_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67215.1"
                     /translation="MRSLAEIFAVCASSGSSFTHAAIEIETSESGCSVEMLRKGMLSR
                     LKDMEFSVNEALSGSWRGLLV"
BASE COUNT          454 a          559 c          579 g          385 t
ORIGIN      
        1 aagcgctgcg acgacggaga gcgaagcggg aagatattgt gcaaggccga tcaggagagg
       61 aagcgagagc ataaggcatg cagcaatcgc acaccgggga cgctccccct tctcgccggg
      121 cgcgaaaaaa gcggcaaaaa gataggtcag ggcatgcacg gcaagaaaaa tgacgaccgc
      181 gttcattcct tcgtactcta ttgctgcaag gcgctgcaat aatccgtact gaagaaagac
      241 ggattccatc caggcaaaaa gcaaccccat gcccgccgct gcggcgagag tcttatatat
      301 cctcaccgga agcctccttc cggaacagag tattgtgctg cattgatgat accatattcc
      361 gtctttttac agggtatctt ctcacccctt tccacatgct taaaaaatgt gatatccttt
      421 ctttcaagat acatattatt tcaaaggggg atgtgttttc atgtccgtaa aaaacgccat
      481 gaccgcagag tttctccgtt ccgcctacgg cggagagagt atggcccaca tgcgctacct
      541 cggctgggct gacaaggcgg ataaagaggg ctttccaaag atcggacgac ttttcagggc
      601 agtcgcctac gccgagcagg tgcacgcgac gaatcacttc gtcgtactga acggcgatgt
      661 cgccgatacc gcgctcactg ccggagccgt cttcggaatg aaggagaccg cggagaatct
      721 cgtcggcggc atcatgggcg aggagcacga agtcgagcag atgtaccctg tgtactacga
      781 ggccgcaaag ttccagggcg aaaaggacgc gatgcggaca ttcaacttcg cgctccaggc
      841 cgagaagcag cacgcggaac ttttccgcgc cgcccgcgag tgcgcccttg ccaagaagga
      901 catggagttc gaggccgtgt acgtctgtcc cgtgtgcggg cataccgtca ccgacgaagc
      961 gccggaaaag tgcccggtgt gcggcgtagc gaaggaaaaa tacgcccgat tctagaagtt
     1021 cttccaaggt agcctgttcc agggggagga gcgcagcttc ctccccctgc ttgctataac
     1081 gcacggaaag ggaggcgagc catgagcttt ctcgatatcg tcggcccggt gatgataggc
     1141 ccctcgtcga gtcatacggc gggagcggcc cgcctgggca gacttgcaaa ggcatgctgg
     1201 gggaccgaca ctcttaacga agtaaccgtc tttctccgcg gcagtttcgc cttcaccagc
     1261 aaaggacacg gcacggacaa ggccgtcgtt gccggtctga tgggaatgct gcccgatgac
     1321 gaacgcatcc ggaacgcgct catgctggcg aaagaagcac actttcctta tcgcctgacg
     1381 accgaggacg tggatggggc gcaccccaac tccgcccgtt tctccttctt ggggaaaaac
     1441 ggacgaagac tcgacgtcgt cggcgcttcg atcggcggag gcgctgtcga aattcaggag
     1501 atcgatggtt tctcggtcgc catcaacggc gagctgccta cgctcatcac atttcataag
     1561 gatacgcgcg gcgttgtggc ggcagttgcc gcgctcatgg cgggacggga gatcaacatc
     1621 gcctccatga cgctccatcg aaagacgcgg ggcggagtgg caaccctcgt cacagagctc
     1681 gatatgaatg cacctcctga cgagcagctg tgtaacgacg tactacaagc gcacccggcg
     1741 attctccgcg tcgcatcgct gtttggcgga ggaaacaatt catgagatcc ctggccgaga
     1801 tcttcgcagt atgcgcatcg tcagggagct cctttacaca cgccgccatc gaaatcgaaa
     1861 cttcggaatc cggctgttcc gttgagatgc tgcggaaggg aatgctctcc cggctgaagg
     1921 atatggagtt ctcagtcaac gaggcgctct ctggctcctg gagagggctt ctcgttc
//
LOCUS       DAOPKU010000566         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_67487_length_1976_cov_3.567933, whole genome shotgun sequence.
ACCESSION   DAOPKU010000566 DAOPKU010000000
VERSION     DAOPKU010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67487_length_1976_cov_3.567933"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /gene="panF"
                     /locus_tag="PK849_13630"
     CDS             complement(<1..942)
                     /gene="panF"
                     /locus_tag="PK849_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015498 - pantothenate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HPE67216.1"
                     /translation="MSTIQWSTLLPIVCYFALMYFIGFYALKLVNNASLRKGGSGAAY
                     MEEYMTGGRGTGGFVLAMTLVATYLSAGSFIGGPGTAYTHGLAWVFLAMSQIPTGYYT
                     LMVLGKKFAIVSRKTGANSISDFLRARYESNAVLIIASLSIIFFLIAAMAAQLIGASR
                     LLQGSTGLDYTTALIFFAITVVVHTAVGGFRGVVLNDTLQGVVMTVSTAALFIAIVMK
                     GGGIANIVQSMKAINPGMISPYGVKEGFMTVPWVSSFWVLVGFAIIGLPAVAQRAMSY
                     KNSKSLHSGIIYGSIVSLVLLMGMHLAGAFGVTQVSGI"
     gene            complement(939..1289)
                     /locus_tag="PK849_13635"
     CDS             complement(939..1289)
                     /locus_tag="PK849_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HPE67217.1"
                     /translation="MTERTESQVLETNEYGFVEDPRYKQCEKEAWYGIILGIVNLVIW
                     AVAGYGLGSGPVEEYSYIMGFPAWFFISCIANSVFAIVATIYLVTRKMRDMPLDPMTK
                     EEAEEFVRNGGKAR"
     gene            complement(1330..>1976)
                     /gene="panB"
                     /locus_tag="PK849_13640"
     CDS             complement(1330..>1976)
                     /gene="panB"
                     /locus_tag="PK849_13640"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPE67218.1"
                     /translation="MEDMIHHTRPVVKGAPDTFVVGDMPFGSYNKSIEQGVENATRLV
                     KETGCDCVKLEGGVKYAPLVRAIVDAGISVMGHIGLTPQNATQLGGFKVQGGTPESAR
                     QLIEDAKELEKAGAFSIVLECVPKVVGKAVSEAVKIPILGIGAGPFVDCQVLVTQDLL
                     GMYGDFKPKFVKLYANVRKVMVDGLNQFHKEALDGEFPSDEYSFNKEVNLEKLY"
BASE COUNT          387 a          614 c          572 g          403 t
ORIGIN      
        1 gattccggag acctgagtga cgccgaaggc tccggcaagg tgcattccca ttagaagcac
       61 gagagagacg atgcttccat agatgattcc cgagtgcagg ctcttcgagt tcttgtagct
      121 catggcgcgc tgcgcgacgg cgggaagacc gataatcgcg aagccgacga gcacccagaa
      181 gctggagacc cacgggacgg tcatgaaccc ttccttcact ccgtaggggg agatcatgcc
      241 ggggttgatc gccttcatcg actgaacgat gttcgcgatt ccgccgccct tcatgacgat
      301 ggcgatgaag agcgccgcgg tggacacggt catgacgacg ccctgaagcg tgtcgttcag
      361 gacgacaccc cggaatcctc cgaccgccgt atgcaccacg acggtgatgg cgaagaaaat
      421 aagagcggtc gtgtaatcaa gccccgttga gccctgcagc aggcgggagg cgccgatcag
      481 ctgggctgcc atggccgcga tgaggaagaa tatgatggag agggaggcga tgatcagcac
      541 cgcgttgctc tcgtaccggg cgcgcagaaa gtcgctgatc gagttggcgc ccgtcttccg
      601 cgagacgatg gcgaacttct tccccagcac catcagcgtg tagtacccgg ttgggatctg
      661 cgacatggca aggaagaccc aggcaagccc gtgggtgtac gccgtccccg gtccgccgat
      721 gaagctgccc gcgctcaggt aggtggcgac aagcgtcatt gcgagcacga aaccgccggt
      781 gccgcgtccg ccggtcatgt actcctccat gtaggcggcg ccgcttcctc ccttgcgaag
      841 actggcgttg ttcaccagct tcagggcgta gaagccgatg aagtacatca gcgcaaagta
      901 gcagacgata ggcagaagcg tcgaccattg gatcgtgctc atcgtgcttt ccctccattc
      961 cgaacgaact cctcggcctc ctctttcgtc atcggatcga ggggcatatc gcgcatcttc
     1021 ctcgtgacga gatagatggt cgcgacgatc gcgaatacgg agttggcgat gcagctgatg
     1081 aagaaccacg ccgggaagcc catgatgtag gagtactcct ccaccggacc gctcccgagc
     1141 ccgtagcctg caacggccca tataacgagg ttgacgatcc cgagtatgat gccgtaccag
     1201 gcctcttttt cacactgttt gtaacgaggg tcttcgacaa agccgtattc gttcgtttca
     1261 agaacctgcg attccgttct ttccgtcatg gtcttccacg acctccttcc tgcttctgcc
     1321 ggcagatgcc tagtacaact tctccagatt cacctctttg ttgaagctgt actcgtcgct
     1381 cgggaactct ccgtcgagcg cctctttgtg gaactggttc agtccgtcca ccatgacctt
     1441 gcggacgttg gcgtacagct tgacgaattt cggcttgaag tcgccgtaca tgcccagcag
     1501 atcctgtgtg acaagcacct ggcagtccac gaaggggcct gcgccgatgc cgaggatggg
     1561 aatcttcacc gcttcggaga cggccttgcc gacaaccttc ggaacgcact ccagcacgat
     1621 ggaaaaggcg cccgccttct caagctcctt cgcgtcctcg atgagctgcc gcgcgctctc
     1681 cggcgttccg ccctgcacct tgaatccgcc aagctgcgtg gcgttctgcg gcgtgaggcc
     1741 gatgtgcccc atgaccgaaa ttcccgcgtc cacgatggcc cgcacaagag gcgcgtactt
     1801 gacgccgcct tccagcttca cgcagtcgca ccccgtctcc ttgaccaacc tcgtcgcgtt
     1861 ttccacgccc tgttcgatgc tcttgttgta cgagccgaac ggcatgtcgc ccacgacgaa
     1921 cgtgtcgggc gcgcctttga cgacgggtct ggtatggtga atcatgtcct ccatgg
//
LOCUS       DAOPKU010000567         1974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_67582_length_1974_cov_4.685774, whole genome shotgun sequence.
ACCESSION   DAOPKU010000567 DAOPKU010000000
VERSION     DAOPKU010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67582_length_1974_cov_4.685774"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK849_13645"
     CDS             <1..201
                     /locus_tag="PK849_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67219.1"
                     /translation="GSDGDVVYYAQVQTVARDGNVFHFKGHLYNVSDKKEIPALFRAT
                     AKAHTFGGKRTWALLSIETVWN"
     gene            complement(286..777)
                     /locus_tag="PK849_13650"
     CDS             complement(286..777)
                     /locus_tag="PK849_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67220.1"
                     /translation="MKKLFALAAVLALALLVSASAFAGTFTLPKAGIDFTLPDSWQPS
                     DSKLGTMFVSPGSDMVIFFGETNSEAGLDGAMELVEQVVSEVLTDIELGEDTEHDING
                     IQVFALEGTGKSDGANAFVSISILNAEGDSLGIMVSFGDEGADQKNAADLQALVGSLK
                     IAQ"
     gene            1119..>1974
                     /locus_tag="PK849_13655"
     CDS             1119..>1974
                     /locus_tag="PK849_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPE67221.1"
                     /translation="MLLFPASEYAERLAKTKERMHEHGVEVLIVSHPANMNYLTGYDG
                     WSFYVHQGLIVSLDCDEPIWFGREQDGNAARILTSLKEENIKGYPDHYVMSKLRHPMQ
                     FVADILRERGWDARHVGVEMEGYYFTAEGFQTLRRELPKGVVKDGTLVVNLVRRIKSA
                     REIEYVRQAARIAENVMKTALDVIAPGVRENDAAAEVYKAQIAGTPEFGGDYTSLCPI
                     MPSAERTSSAHLTWCADRKYREGDIVLLELAGARHHYHCPISRTLFLGEPPAELRKTA
                     DAVVKGLNE"
BASE COUNT          441 a          586 c          585 g          362 t
ORIGIN      
        1 ggatccgacg gcgacgtggt ctactacgcg caggtacaga cggtcgcccg cgacgggaac
       61 gtcttccact tcaaggggca tctgtacaac gtctccgaca agaaggagat acccgccctc
      121 ttccgcgcca cggcgaaagc gcacaccttc ggcggaaaga ggacctgggc gctgctctcg
      181 atagagaccg tctggaacta acccctcccc ggagggtgga gtacggcata aaaaaggagg
      241 agccttcgcg gctcctcctt cgctgtttcc gtgtgctttt agttcttact gggcgatttt
      301 cagcgagccg acaagagcct gaaggtcggc ggcgttcttc tggtcggcgc cctcgtctcc
      361 gaaggagacc atgatgccga gcgagtcgcc ttcggcgttc aggatgctga tgctgacgaa
      421 ggcgttcgcg ccgtcggact tgccggttcc ctcgagggcg aagacctgga tgccgttgat
      481 gtcgtgctcg gtgtcctcgc cgagttcgat gtcggtcagg acttcgctca caacctgctc
      541 gacgagctcc atcgcgccgt cgagtcccgc ttcgctgttc gtttcaccga agaagatgac
      601 catgtcgctg ccgggagaga cgaacatcgt gccgagcttg gagtcggaag gctgccatga
      661 atcgggaagc gtgaagtcga tcccggcctt cggcagggtg aaggttccgg cgaacgccga
      721 cgcggagacg agaagcgcaa gagcaagaac agctgcaagt gcgaaaagct ttttcatggt
      781 acggtgaatc ccccttgtga atgatattat gatgtgctgc tcaaacgaga agacacgaag
      841 ctgcttcctt ccatgttctt ccatttttta cgcaaaagat acatagactc gattcaaata
      901 tacttgaagc atcagttcaa gagaaaacag gaaacgcgta caaaaacaca gtacaagcgg
      961 aagaatagca cgggaagcga actttggcaa gaaaaacttt tgttacatac aaaaaaaata
     1021 cacgggctcc cgcgtcttga caaaccgctt tttacggggt attgttttat tgtcgacaaa
     1081 cgacataaaa acgaaaactt ccacaaggcg gtgacaagat gttgctcttt ccggcttcag
     1141 agtacgctga gcgcctcgcg aaaacgaagg aacgaatgca cgagcacgga gtggaagtgc
     1201 tgatcgtctc ccaccccgcc aacatgaact acctcacggg gtacgacggc tggtccttct
     1261 acgtccatca gggcctgatc gtctccctcg actgcgacga gccaatctgg ttcgggcgag
     1321 aacaggacgg caacgccgcg cgcatcctca cctccctcaa agaggagaac atcaagggct
     1381 accccgacca ctacgtcatg tcgaagctgc gccacccgat gcagttcgtc gcggacatcc
     1441 tccgcgaacg cggatgggac gcgcgccacg tcggcgtcga gatggagggg tactacttca
     1501 ccgccgaggg cttccagacg ctccggcgcg agcttccgaa gggtgtcgtg aaggacggga
     1561 cgctcgtggt caacctcgtc cgcaggatca agtcggcgcg cgagatcgag tacgtccggc
     1621 aggcggcgcg catcgccgag aacgttatga agacggcgct cgacgtcatc gcccccggag
     1681 tccgcgagaa cgacgcggcc gccgaggtct acaaggcgca gatcgcgggc acgcccgagt
     1741 tcggcggcga ctacacctcg ctctgcccga tcatgccgtc ggccgagcgc acctcgtcgg
     1801 cacacctcac ctggtgcgcc gacaggaagt accgggaggg ggacatcgtc ctgctcgaac
     1861 ttgcgggggc gcgccatcac tatcactgcc ccatatcgcg cacgctcttc ctcggggagc
     1921 cgcccgcgga gcttcggaag acagcggacg ccgtcgttaa gggcctgaac gaga
//
LOCUS       DAOPKU010000568         1973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_67632_length_1973_cov_5.383733, whole genome shotgun sequence.
ACCESSION   DAOPKU010000568 DAOPKU010000000
VERSION     DAOPKU010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67632_length_1973_cov_5.383733"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="ilvC"
                     /locus_tag="PK849_13660"
     CDS             complement(<1..791)
                     /gene="ilvC"
                     /locus_tag="PK849_13660"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPE67222.1"
                     /translation="MAKALYDKDANMKLLEGKTVAVLGYGSQGHAHSQNLRDSGVSVV
                     VGLHEGSKSKATAEKDGFEVYTVAEATKRADVVMFLMPDHVQGDTYRKHVAPNLKKGA
                     ALAFAHGFSVHFNQVEPSKDNDVFMVAPKGPGHLVRRMYTEGRGVPCLLAVQQDASGQ
                     ARDLGLAYASALGGGRAGVLSTTFKEETETDLFGEQAVLCGGITELIKAGFDTLVEAG
                     YQPEIAYFECLNEMKLIVDLMYEGGMGWMRFSVSDTAKYGDLTAG"
     gene            complement(823..1314)
                     /gene="ilvN"
                     /locus_tag="PK849_13665"
     CDS             complement(823..1314)
                     /gene="ilvN"
                     /locus_tag="PK849_13665"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPE67223.1"
                     /translation="MERTIGVVAENRPGVFSRISGLMERRGFQVKGVTAGVTQQPGRS
                     RFTLVIAGDDRKASQAVRQIRKMVETVEVSDLSSGFCVERCMMLMKFSLPAERRAELF
                     DAMNAFPHTVASEHGDTVILETTGTGENLDACLETVRHLGLVEAVKSGPLALDARTTT
                     TTF"
     gene            complement(1451..1660)
                     /locus_tag="PK849_13670"
     CDS             complement(1451..1660)
                     /locus_tag="PK849_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67224.1"
                     /translation="MPAIRTARNERPSRRTPWYHTVGKDDIEAKEETPVKNMSTTTHS
                     ALTFVVAGAIIIIRCGDSPGALLVV"
     gene            1739..>1973
                     /locus_tag="PK849_13675"
     CDS             1739..>1973
                     /locus_tag="PK849_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPE67225.1"
                     /translation="MDVEKLRECREKINEIDGSIAALLSQRADVAREVGRAKGDAPVY
                     DPAREGEVIARAAEACPGMDRSAVERIYREIISV"
BASE COUNT          331 a          596 c          643 g          403 t
ORIGIN      
        1 tttccggcgg tgaggtcgcc gtacttcgcc gtgtcgctga cggagaaacg catccacccc
       61 atgccgccct cgtacataag gtcgacgatc agcttcatct cgttgaggca ctcgaagtag
      121 gcgatctccg gctggtatcc ggcctcgacg agggtgtcga atcccgcctt gatcagctcg
      181 gtgatgccgc cgcagaggac ggcctgttcg ccgaagaggt cggtctcggt ctcttccttg
      241 aaggtggtgg agagcacgcc cgcccgtccg ccgccgagag cggaggcgta ggcgaggccg
      301 aggtcgcgcg cctgtcccga ggcgtcctgc tggacggcga ggaggcaggg aactcccctt
      361 ccctcggtgt acatgcgccg gacgaggtgg ccgggaccct tgggggcgac catgaagacg
      421 tcgttgtcct tggacggctc gacctggttg aagtggacgg agaagccgtg cgcgaaggcc
      481 agcgccgcgc ccttcttcag attgggggcg acgtgcttgc ggtaggtatc gccctgcacg
      541 tggtcgggca tcaggaacat gaccacgtcg gcgcgcttcg tcgcctcggc gacggtgtac
      601 acttcgaagc cgtccttctc ggcggtcgcc ttcgacttgc tgccctcgtg gaggcctact
      661 acgaccgaga ctccgctgtc gcggaggttc tgcgagtgcg cgtgcccctg gctgccgtag
      721 cccagaaccg cgacggtctt tccttcgaga agcttcatgt ttgcgtcctt gtcgtagagt
      781 gcctttgcca taatcagtcc aactcctttt cgaatgtgtg ctttagaatg tggtggtcgt
      841 cgtccgcgcg tcgagggcca gggggccgct cttcaccgct tcgacgagcc cgagatgccg
      901 aaccgtttcg aggcacgcgt caagattctc gcccgtgccg gtggtttcga ggatcaccgt
      961 gtcgccgtgc tcgcttgcca cggtgtgggg gaaggcgttc atcgcgtcga agagctccgc
     1021 gcgccgctcc gccggaaggg agaacttcat cagcatcatg cagcgctcca cgcagaagcc
     1081 cgagctgaga tcggagactt cgacggtctc caccatcttg cgtatctgcc gcaccgcctg
     1141 ggacgccttg cggtcgtcgc ccgcgatgac gagggtgaag cgggaacgcc ccggctgctg
     1201 cgtgactccg gcggtgaccc ccttcacctg aaatcccctg cgctccatga gccccgatat
     1261 gcgtgaaaaa actcccggtc tgttttccgc gacgacgccg atggtccgtt ccatgccgct
     1321 ctcctcctct tatcttgtgt atgcccctct cgggagctct ctcttcgcct gcgaaggcgg
     1381 tatctgcgca taaaaaaagc cggggttccc gtagagtgcg ggaaccccgg ccggaaggct
     1441 cttcgtcggt ctacacgacg agaagcgctc cgggggaatc cccgcagcga ataataataa
     1501 tggcgccggc tacgacaaat gtaagggcgg agtgcgtcgt tgtggacata ttcttcacag
     1561 gggtttcctc cttcgcttcg atatcgtctt tcccgaccgt gtggtaccat ggagttctcc
     1621 gagaggggcg ctcgttccgc gccgttcgga ttgcgggcaa tcttataggc ctccttcgaa
     1681 aatgtcaagg ggggttgaaa taaaagataa ttctaaaatt ttcagagggg gttgttctat
     1741 ggacgttgaa aagcttcgtg aatgccggga aaagatcaac gagatcgacg ggagtatcgc
     1801 cgcgcttctt tcgcagcggg cggacgtcgc ccgcgaggtg gggagggcga agggggacgc
     1861 tccggtgtac gaccccgcga gggagggcga ggtgatcgcg cgcgcggccg aggcctgccc
     1921 ggggatggac cggagcgcgg tcgaacggat ctaccgcgag atcatctccg tct
//
LOCUS       DAOPKU010000569         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_68291_length_1960_cov_3.974278, whole genome shotgun sequence.
ACCESSION   DAOPKU010000569 DAOPKU010000000
VERSION     DAOPKU010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68291_length_1960_cov_3.974278"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PK849_13680"
     CDS             complement(<1..412)
                     /locus_tag="PK849_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPE67226.1"
                     /translation="MKMNLRKVCAFVLIAAFLFTGAGTGTAAEVLRMATTTSTDNTGL
                     LDYLAPLLLNEKGIEIRWVSVGTGKALEYGRNGDVDVLLVHDPEAEAKFVAEGSGVNC
                     RQVMFNDFIIVGPVSDPAKVQGLSVTAALKKIAQD"
     regulatory      complement(484..603)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     regulatory      746..865
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            919..1713
                     /locus_tag="PK849_13685"
     CDS             919..1713
                     /locus_tag="PK849_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE67227.1"
                     /translation="MPRSLRERLFMLSMGSFLLLYILWPLGGLFLRADWSLVASASHD
                     GEVLSALWRSIWTAAAATGVIALFGTPLAYFLARQSFRGKSILEAVIDVPIMIPHTVA
                     GIAVLMAFSPKAPAGALLTSMGFSPVNSNLGIILACMFVSIPFYVDAARDAFYSVSPR
                     TEKVARTLGASFPHVFFRISLPLARKGLISGLIMSWARGVSEFGAIVIIAYHPMVAPT
                     LIYDRFATFGLKYSTPVAVQLILVCLGMFVVLRILAAGKRKEDAEI"
     gene            1710..>1960
                     /locus_tag="PK849_13690"
     CDS             1710..>1960
                     /locus_tag="PK849_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE67228.1"
                     /translation="MIELENLSVNLRTFRLESLSLRIAPGEFFMIVGPSGAGKTILLE
                     SIAGLRSLEGGRVRIRGLDVTPLPPERRNVALVYQEYAL"
BASE COUNT          348 a          616 c          559 g          437 t
ORIGIN      
        1 cgtcctgcgc gatcttcttc agcgccgccg tcaccgagag tccctgcacc ttcgccggat
       61 cggaaaccgg gccgacgatg atgaagtcgt tgaacatcac ctgtcggcaa ttcactcccg
      121 aaccttcggc gacgaacttc gcctcggcct cgggatcgtg gacgagaagg acgtccacgt
      181 caccgttgcg gccgtactca agcgccttcc ccgtaccgac ggagacccag cggatctcga
      241 tgcccttttc gttcagaagc agaggggcga ggtaatcaag cagtcccgtg ttgtcggtgc
      301 tggtggtggt ggccatgcgg agcacctccg ccgccgttcc cgtaccggct ccggtaaaaa
      361 gaaatgccgc gatcaataca aaagcacata ccttcctcaa attcattttc attcctcctc
      421 gatatgattt tgtcccgcat acaaaaagcc gcctccccca tatacggcgg gagacggctg
      481 catcagcatc cgaatccatc tccttgccga acacgggagg ctcgcctgtt tccggacgaa
      541 ccctgacggc ccgccgccgt gggaatttcc tgtaggcaaa cgggctcgga gaacaagttg
      601 tcttttattt gatgaatatc ataccataaa acctcgagtc gaggtatccg ccgacaggca
      661 tgagaacgag catttttcgc ctttacttga taaagagtat cattgtcatt ttctgaacta
      721 cgtggtattc ttgcttcgtc cgggtttttc ggacatcctc cgagccgcac tccctacgga
      781 atacgttcca tgggagacgg ccaacgggtt ctcccggaaa cgggagggcc tcccgcgttc
      841 ggaaaggagg agcggccggt gtatcccagg gctgtctccc atggagacgg ccctttttat
      901 gtgtctggag ggtggaatat gcctcgttcg cttcgggagc gtttgttcat gctgtccatg
      961 ggttccttcc tgctgctgta tattctctgg ccgctcggcg gactctttct gcgcgccgac
     1021 tggagtctcg tcgcctcagc gtcgcatgac ggggaggtgc tctccgcact gtggcgcagc
     1081 atctggacgg ctgcggcggc gacgggagtc atcgcgctct tcgggacgcc tcttgcctac
     1141 ttcctggcgc gccagtcctt tcgcggaaag tcgatcctcg aagcggtcat cgacgtgccc
     1201 atcatgattc ctcacactgt ggcaggcatc gcggtactca tggccttctc gccgaaggct
     1261 ccggcggggg cgctgctcac ctccatggga ttctctcctg tgaacagcaa tctcggaatc
     1321 atcctcgcat gcatgttcgt ctccattccg ttctatgtgg acgcggcgcg cgacgccttc
     1381 tactcggtct ccccgcggac ggagaaagtg gcccggacgc tcggggcctc attccctcac
     1441 gttttcttcc gcatctccct ccccctggcg aggaaaggat tgatctccgg cctcatcatg
     1501 agctgggcgc ggggagtgag cgaattcggg gcgatcgtca tcatcgccta ccacccgatg
     1561 gtggccccca cgctgatcta cgaccggttc gccaccttcg gactgaagta ctccacgcct
     1621 gtggccgtcc aactcatcct cgtctgcctc ggcatgttcg tcgttctgcg gatactggcc
     1681 gcagggaagc ggaaggagga tgccgagata tgatcgagct tgaaaatctc tcggtcaatc
     1741 tccgcacctt ccggctagaa tcgctctctc tgcggatagc cccgggcgag ttcttcatga
     1801 tcgtggggcc aagcggcgcg ggaaagacga tcctgctgga atcgatcgcc ggactgcgct
     1861 ccctcgaggg gggaagggtc aggatacggg ggctcgacgt cacgcccctg ccgcccgagc
     1921 ggcggaacgt cgcgctcgtc taccaggagt acgcgctctt
//
LOCUS       DAOPKU010000570         1959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_68321_length_1959_cov_4.714811, whole genome shotgun sequence.
ACCESSION   DAOPKU010000570 DAOPKU010000000
VERSION     DAOPKU010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68321_length_1959_cov_4.714811"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PK849_13695"
     CDS             complement(<1..322)
                     /locus_tag="PK849_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPE67229.1"
                     /translation="MLTRILFFADVSSFSERALAWAARQVAGDRSDFLILHVVDRAAG
                     LDAPELVREAEGYLEEMCARTLPPESYYKTLVLLGDLLESLPDTIRSEKCTFAIFPLP
                     GGEDG"
     gene            384..1262
                     /locus_tag="PK849_13700"
     CDS             384..1262
                     /locus_tag="PK849_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPE67230.1"
                     /translation="MKDFTEVKWKALSGVLRLLKRMPLTWSVRFGASLGWLLWALDKK
                     KVDEAERRCVRALQVGPTIARGIVRRSYMNLGRSVAEFAAVPGSGRSMRSIVHVHGEE
                     HLREALDSGNGVIFFSGHIGNWEFGAAVLAEMGYPMNAIGADQRDERVTDLIIGLRAV
                     SGVTTVSKGFDLKAAIRCLKRGEALAILIDQDVRERGVVVPFLGIPASTPYGPAKIAG
                     KIGSVILPSFMLRRGTSEEHDLHILPSPWKNGRPERDESMEEAMARCNDLISEYIRRA
                     PDQWMWLYPRWASTGE"
     gene            1265..1720
                     /locus_tag="PK849_13705"
     CDS             1265..1720
                     /locus_tag="PK849_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPE67231.1"
                     /translation="MFLGIDPGRDKVGWALTDAEGELLLSGIFRAGDAALFFSAFESG
                     ALERLAPFILEKTSGEIADFASAELLMGDGTGKEYLLSLAQSASLGVKLVSERETTLL
                     ARKLYWKLHPPRGVWRLLSAGLRVPPRDIDDLAAWAIVLRYCGVQSKEK"
     gene            1741..>1959
                     /locus_tag="PK849_13710"
     CDS             1741..>1959
                     /locus_tag="PK849_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPE67232.1"
                     /translation="MGTEKLATLVNTFGSSLMTVSKEMGVELTLAKSEIAQGVNVQGS
                     CLAALVGVVGGGVQGTAVIMLDEGGFRAT"
BASE COUNT          394 a          496 c          654 g          415 t
ORIGIN      
        1 tcccgtcctc cccgccggga agagggaaga ttgcgaacgt gcacttttcg gagcggatgg
       61 tatcgggaag agactcaagc agatctccca gaaggaccag ggtcttgtag taggattcag
      121 gggggagcgt tcgcgcgcac atctcttcca ggtacccctc ggcctccctg accagctcgg
      181 gagcgtcgag ccccgcggcc cggtcgacta catgaagaat caggaaatcc gaacgatctc
      241 ccgcgacttg cctggccgcc catgccaggg ctctttcgga aaatgaggag acgtctgcga
      301 aaaagagaat tcgagtgagc atgtttcatc cctcccatgg tattgtatgt gtgctttaag
      361 tatagcgcaa aggggttgcg gcggtgaagg attttactga ggtaaaatgg aaggccttgt
      421 cgggtgttct tcggctcttg aagagaatgc cgttgacttg gtcggtgcgc tttggagcgt
      481 cgctcgggtg gctgctctgg gcgctcgata agaaaaaggt ggatgaggcg gaacggcggt
      541 gtgttcgcgc gcttcaggtg gggccgacga tcgccagggg aatagtccgc agatcctaca
      601 tgaacctggg gcgttccgtt gctgagttcg ccgcagtgcc gggaagcggt agaagtatgc
      661 gctcgatcgt gcatgttcac ggtgaggaac accttcgcga ggcattggat tccggaaacg
      721 gcgtcatttt tttctcgggg cacatcggaa actgggagtt cggcgcggcg gtgctggcgg
      781 agatgggata cccgatgaac gccatcggcg cggaccagcg cgacgagaga gtgaccgacc
      841 ttatcatcgg acttcgcgcg gttagcgggg tcacaaccgt gagcaaagga ttcgacctca
      901 aggctgccat acgatgcctc aagaggggcg aagcccttgc catcctgatc gatcaggatg
      961 ttcgtgagag aggcgtcgtc gtcccgtttc tggggatacc cgcgagcaca ccctacggtc
     1021 cggcgaagat cgccgggaaa atcggctccg tcatcctgcc gagttttatg ctgcggaggg
     1081 gaacctcgga ggagcacgat cttcatattc tgccctcccc gtggaaaaac gggaggccgg
     1141 aacgggacga atcgatggag gaggccatgg cccgctgcaa cgatctgatc agcgagtata
     1201 tccgccgtgc tcccgaccag tggatgtggc tctatccccg atgggcgtcg acgggagagt
     1261 agcgatgttt ctcgggatcg accccggcag ggacaaagtc ggatgggccc ttacggatgc
     1321 tgagggagag cttctcctgt cggggatttt ccgggcgggg gacgcggctc tttttttcag
     1381 cgcgttcgaa tccggggcat tggagcggct tgcgccgttc attcttgaaa aaacctccgg
     1441 tgagattgcg gattttgcgt ccgccgagtt gcttatggga gacggaactg gaaaagaata
     1501 ccttctctct cttgcgcaat ccgcctctct tggagtaaag ttagtgtctg aaagagagac
     1561 aacgctcctg gcgaggaaac tctattggaa actccatcct ccgcgcggtg tgtggagact
     1621 gctttccgcc ggtctccgcg ttcctccgag ggatatagac gatctcgccg catgggctat
     1681 cgttcttcgc tactgcggtg tgcagtcgaa ggagaagtag aaagggaagg gtgaaagcaa
     1741 atgggaacgg agaagctggc gacgcttgtt aatacattcg ggtcttctct gatgacggtc
     1801 agcaaggaga tgggcgttga gctgactctc gcgaagtccg agatcgccca gggagtgaat
     1861 gttcagggga gctgccttgc cgcgcttgtt ggcgtcgtcg gaggtggcgt ccagggaacc
     1921 gccgtgatca tgttggacga ggggggcttc cgggcgacg
//
LOCUS       DAOPKU010000571         1956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_68461_length_1956_cov_5.009469, whole genome shotgun sequence.
ACCESSION   DAOPKU010000571 DAOPKU010000000
VERSION     DAOPKU010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68461_length_1956_cov_5.009469"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="PK849_13715"
     CDS             complement(<1..1787)
                     /locus_tag="PK849_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPE67233.1"
                     /translation="MALVKENMHITLKQAWTLLREAITQIAPELCSVQHCYERLLAAD
                     LLSQRNVPHYNASAMDGFALSSASTAHATPSSPVFLRRDEFARLNTGGVLPASFDAVA
                     MIEDVALSSDGEKLTVAKTLVAGENVRPVGEDVFRGQVIARVGERISPPLASLCATAG
                     IPSVSVQALPKTLFIPTGDEIIPLEEWLSMDEPPAGKVGESNSFLVQGYFSRWGFPVD
                     IAPCLPDNPEVLSEFLLKKAEEYDLLLVGAGSAKGEKDYTFSIFEKYGKPIFRWLLMK
                     PGRPASAALLRRAVAVNLPGFPMSNAVILWSIVYPILQLLQKGDFDESSAIQEALGAE
                     GSEEMSLLVSCSSSPGKEEWLRLKCVEIDGRKMAYPLPSGASALWSLAEIDAVSLLPL
                     ESAELPKGSAVSVWTTRRIPWKERILFQGSNDPALERLPSFVRKQGGDLLLRSVGSLA
                     GLAALSRRECHIAAAHLFHAETGEYNTPFIDELFHDPETVVRRRVFFREQGFITKKGN
                     PKKISGVRDLDREDIVLVNRQRGAGTRVLLDALLDEAGIHPSAVRGYNSLSPTHFESA
                     NRVALGFVDVALGIKSVADALDLDFIPVA"
     gene            complement(1775..>1956)
                     /locus_tag="PK849_13720"
     CDS             complement(1775..>1956)
                     /locus_tag="PK849_13720"
                     /inference="COORDINATES: protein motif:HMM:NF015419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67234.1"
                     /translation="FGHSGIEEFLPCALRDGKAVPLPVKSSFAKALGQADGILRLSSS
                     RETVRPGEEVEVWLW"
BASE COUNT          478 a          538 c          511 g          429 t
ORIGIN      
        1 tccgcaactg gaatgaaatc caagtcaagc gcgtcggcga cagattttat accaagagca
       61 acgtcgacaa agcctaacgc tactctgttg gctgattcaa aatgtgtcgg actcagacta
      121 ttgtatcccc tgaccgcgga aggatgaatg cccgcttcgt ccaaaagcgc gtccagcagc
      181 acacgggttc ccgcgcctcg ctgtcggttg acgagcacaa tatcctcccg gtcaagatct
      241 cgtaccccgg agatcttctt ggggttgcct tttttcgtaa taaaaccctg ttcccgaaag
      301 aaaactctcc tccggacaac cgtttccgga tcatgaaaaa gttcgtcgat gaacggagtg
      361 ttgtactctc ccgtctcggc atggaataga tgcgccgccg cgatatggca ttctcttctc
      421 gaaagagcgg caagtcccgc aaggcttccg accgatctga gcaggagatc gccgccctgt
      481 ttgcggacaa atgaggggag gcgttccaag gcaggatcat ttgacccttg gaaaagaatg
      541 cgttccttcc agggaatacg ccgggtcgtc cacaccgaaa cggcgcttcc cttcggcaat
      601 tccgcgcttt ccaacggaag cagcgacact gcgtcaatct ccgccaagga ccagagtgcg
      661 cttgcccctg aaggcagcgg atacgccatt tttcgcccgt ctatctccac gcattttaaa
      721 cgaagccatt cctcttttcc cggcgaggaa gagcaggaga cgagaagaga catctcttcg
      781 gatccttccg ctccgagagc ttcttggatt gcggaagatt cgtcaaaatc ccctttctga
      841 agaagctgta aaatcgggta gacgatcgac cagaggatga cagcgttcga cattggaaaa
      901 cccgggagat tgaccgcgac tgcacgtcgt agcagcgcag cggatgcagg tctgcctggt
      961 ttcatcagaa gccatcgaaa gattggcttc ccgtattttt caaagatgga aaaagtatag
     1021 tccttttctc cttttgccga gccggcccca acgagcagca ggtcgtactc ctcggctttt
     1081 ttcaaaagaa actccgaaag tacctctgga ttgtcgggaa ggcatggagc tatatcgaca
     1141 gggaaacccc atcgggaaaa gtagccctga acgagaaatg agttgctctc ccctactttc
     1201 ccagcaggcg gctcgtccat ggaaagccat tcctctagag ggatgatttc atctcctgta
     1261 gggatgaaga gagtcttcgg aagagcctgt acgctgactg aaggaattcc cgccgtggcg
     1321 cacaaggatg ccagcggcgg cgatattctc tcccctacac gcgctattac ctgtcctcga
     1381 aagacatctt caccaacagg acgaacattt tctccggcaa cgagcgtttt ggcgacggtc
     1441 agcttttctc cgtcactgga gagcgccacg tcctcaatca tagcaaccgc atcgaaggat
     1501 gcaggcagca caccgcccgt attgagccga gcaaactcgt cgcggcgtaa aaatacaggc
     1561 gaagatggcg tcgcgtgggc agtggaggct gatgagagtg cgaatccgtc catggcggat
     1621 gcattgtagt gcggaacgtt tctttgggag agaagatccg cggcaagtaa tcgttcataa
     1681 caatgttgta cagaacataa ctcgggggcg atctgtgtga ttgcctcccg aagaagagtc
     1741 cacgcctgtt tcagtgtaat gtgcatattt tcctttacca aagccatact tctacctcct
     1801 cgccgggacg gacagtctct ctcgaagacg aaagacggag aatcccgtcg gcttgtccga
     1861 gcgcctttgc gaacgaagat ttcaccggaa gaggaacagc ctttccgtcg cggagggcgc
     1921 atggtaaaaa ttcctcaatc cccgagtgac cgaata
//
LOCUS       DAOPKU010000572         1948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_68869_length_1948_cov_4.885895, whole genome shotgun sequence.
ACCESSION   DAOPKU010000572 DAOPKU010000000
VERSION     DAOPKU010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68869_length_1948_cov_4.885895"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            515..787
                     /locus_tag="PK849_13725"
     CDS             515..787
                     /locus_tag="PK849_13725"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPE67235.1"
                     /translation="MDSFGRAVENSMDRMKARLKTPEGRPEYGNRKSVVVPVFGIIKE
                     VLKCRRFLLRGMKKAKGEWTLACMAYNLKRMNILKTLHERKHPGRG"
     gene            859..1092
                     /locus_tag="PK849_13730"
     CDS             859..1092
                     /locus_tag="PK849_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67236.1"
                     /translation="MIRSCRGQSYRLLAIDDTRQVYIRDDGIDRLRELLDDLEIPEDV
                     EGSPLAVVRACGEHLLIVWCEFSGGGGVRGEKS"
     gene            1079..1480
                     /locus_tag="PK849_13735"
     CDS             1079..1480
                     /locus_tag="PK849_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67237.1"
                     /translation="MKSLEGSVAGKMLCAFVGTAFLLVAAAWSEERVSVRYLCNGEKV
                     IPVVCSIGNWRITDIKLPDLVLTNNGGTALDVECAEILGFVSGTEAVRVRISGGELRE
                     AIRNTAGWLGDPNTPIMDPENRTKNRGEVKL"
     gene            1484..1786
                     /locus_tag="PK849_13740"
     CDS             1484..1786
                     /locus_tag="PK849_13740"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPE67238.1"
                     /translation="MVARRNFSAEFKTKVVLEMLREEETLSQIAARHKIHHTVLQNWK
                     KTVTEGMPGLFADPRKKSAEDLQKDSTMNDLYKQVGLLSMQLEWLKKKCGVDSFSS"
     gene            1764..>1948
                     /locus_tag="PK849_13745"
     CDS             1764..>1948
                     /locus_tag="PK849_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPE67239.1"
                     /translation="MSTLSLHERRALVDWENKDFPLSWQAWLLGLNRTGLYYVPGPLS
                     EEELLLRRRIDEIHTDQP"
BASE COUNT          483 a          433 c          617 g          415 t
ORIGIN      
        1 tccaatgcgc ctgtagctca gcggatagag tatcggcctc cggaattgca gactgtttct
       61 accttttttt gaaaattacg tgaagcattg ctattatcga cagaaaggga cttccttaaa
      121 cgggagtcct ttttttgtgc atcgaccttt cgaatatttc ttaataccat ctgttttgtt
      181 atgttaggaa aaaagtcttt cagttgtttt cctcccctct acaaagggat cccggagtac
      241 tttgtgcgca tcctgaaagt ggcgcgggag atgggattga ggaaagtggg aagcgtcagt
      301 ccggacggga cgaaggtcaa ggcaaatacc ggctaatacg aggagcgggg aatcgaactg
      361 cgtatctcca tcggaagaga acagcacaac atgtcgtttg ttgaaagatg cggggaaagc
      421 gaggaaatgt ctgccgcgac agaagaggcg gagaggtcgt ccgggttgcg aagtcgagtt
      481 ttgcgaggaa agggtgggga agtcgagtcc gactgtggat agttttggtc gcgctgtgga
      541 gaactcgatg gaccgcatga aggccaggct gaaaacgccg gaggggagac cggagtacgg
      601 gaatcggaag agtgttgtcg tgcctgtctt cgggatcata aaggaagtct tgaagtgcag
      661 acggtttctt cttcgaggga tgaagaaagc gaagggagaa tggacgttgg cgtgcatggc
      721 gtataatttg aagagaatga acatactgaa gacattgcac gagagaaaac accccggaag
      781 aggataattc tccccccagg gtgctgaaat ggacgctttc gtatccggac gtcgagaaat
      841 ccggagttgt tggcgcttat gatacggagt tgtagaggcc agtcctacag actcctagcc
      901 atagacgaca cgagacaggt ctatatccgc gacgatggaa tcgatcgatt gcgggagctt
      961 ctagacgatc tcgagatacc ggaggacgtc gaagggagtc cgctcgccgt cgtccgggcc
     1021 tgcggggagc atttgttgat cgtatggtgc gagttcagcg gcggaggagg agtgcgaggt
     1081 gaaaagtctt gagggaagcg tagcgggaaa gatgctgtgt gcctttgtcg gaacggcgtt
     1141 tcttctcgtc gcggcggcgt ggtcggagga acgggttagt gtacggtatc tctgcaatgg
     1201 ggaaaaggtg attccggtgg tctgcagcat cggaaactgg cggatcaccg acatcaagct
     1261 gcccgatctg gttcttacga ataacggggg gactgctctg gatgtcgagt gtgccgagat
     1321 actcgggttc gtctccggta cggaggcggt gcgtgtgcgc atatccggcg gggagctgcg
     1381 cgaagccata cggaataccg ccggctggct cggcgacccg aacaccccta taatggaccc
     1441 cgaaaatcgg acaaaaaatc ggggggaggt taagctataa aagatggtag cgcgtcggaa
     1501 cttttcagcg gaattcaaga caaaagttgt gcttgagatg cttcgagaag aagaaacgct
     1561 cagtcagatc gcggccaggc ataaaatcca tcacacagtt ctgcagaact ggaagaagac
     1621 ggtcaccgaa ggaatgccgg gcctgttcgc cgatccccga aagaagagcg cggaggatct
     1681 gcagaaggat tctacgatga acgacctcta caagcaagtc gggctgctct ccatgcagct
     1741 tgagtggctc aaaaaaaaat gcggtgtcga ctctttctct tcatgaacgc agggcgctgg
     1801 tggactggga gaacaaggat ttccccctct cctggcaggc ctggctgctc ggactcaaca
     1861 gaacaggact ctactacgtt cccgggcctc tgtccgagga ggagctgctg ctccgcaggc
     1921 ggatagacga gattcacacc gaccagcc
//
LOCUS       DAOPKU010000573         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_69092_length_1944_cov_3.595024, whole genome shotgun sequence.
ACCESSION   DAOPKU010000573 DAOPKU010000000
VERSION     DAOPKU010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69092_length_1944_cov_3.595024"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1944
                     /locus_tag="PK849_13750"
     CDS             <1..>1944
                     /locus_tag="PK849_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE67240.1"
                     /translation="EGKTSAAGRILADLGATEEKFLSVLNEVRGRARVCSDNPESTYE
                     ALEKYGRDLVAMARAGKLDPVIGRDEEVRRVIRILSRKTKNNPVLIGDPGVGKTAIVE
                     GLAGRIVKGDVPEGLKDRSIFALDMGSLVAGAKFRGEFEERLKAVLNEVKESDGRIIL
                     FIDELHTIVGAGAAEGAIDAGNMLKPLLARGELHCIGATTVDEYRKRIEKDAALARRF
                     QPVLVEQPTVEDTVSILRGLKDRFQVHHGVKIRDNALVAAAVLSNRYITERFLPDKAI
                     DLVDEACALIRTEIDSLPAELDAVSRKVMQLEIEEAALNKEKDAASVERLKALQKELQ
                     EAREEQTGLRAQYEAEKQGIGVVRELRERIESIRRDIEKAEREYDLNRAAELKHGTLP
                     QLEAELREREKTLENGHAGPKILREEVTEEEISEIVSRWTGIPVTRLLEGEKEKLLQL
                     DEVLHRRVVGQDEAVELVADAVLRARSGIKDPRRPIGSFIFLGPTGVGKTELAKTLAE
                     SLFDSEENLIRLDMSEYMEKHSVSRMVGAPPGYVGYDEGGQLTEAVRRRPYSVILFDE
                     IEKAHPDVFNILLQILDDGRITDSQGHLVNFKNTVVIMTSNIGAQYLLEGITSDGQIR
                     ESARTQVMDELRRAFRPEFLNRV"
BASE COUNT          406 a          573 c          668 g          297 t
ORIGIN      
        1 ggagggcaaa acaagcgccg ccgggcggat tcttgcagat ctcggggcga cggaggagaa
       61 gtttctctcc gtcctcaacg aagtgcgcgg aagggcgcgc gtctgcagcg acaaccccga
      121 gagcacctac gaggcgctgg agaagtacgg ccgcgatctg gtggcgatgg cccgcgccgg
      181 aaagctcgac cctgtcatcg ggcgcgacga agaggtccgc agggtgattc gcatcctgag
      241 caggaagacg aagaacaatc ccgtgctcat cggcgacccc ggcgtcggaa agacggccat
      301 cgtcgagggt ctcgcagggc gcatcgtgaa gggcgacgtc cccgaggggc tgaaggaccg
      361 ttccatcttc gcgctcgata tggggtcgct cgtcgcgggg gccaagttca ggggcgagtt
      421 cgaggagcgg ttgaaagcgg tgctcaacga agttaaggag agcgacggcc ggatcatcct
      481 cttcatcgac gaactgcata ccatcgttgg cgcgggcgcc gcggaaggcg ccatcgacgc
      541 ggggaacatg ctcaaacctc tgcttgcacg cggcgaactg cactgtatcg gcgcaaccac
      601 agtggacgag taccgcaagc gcattgaaaa ggacgcggcg ctcgccaggc gcttccaacc
      661 cgttctcgtt gagcagccga ccgtcgagga tacagtgtcc atactgcggg gcctcaagga
      721 ccgctttcag gtgcatcacg gtgtgaagat acgcgataac gctctcgtcg cggcggcggt
      781 gctctccaac cgctatatca ccgagcgatt tctcccggac aaggccatcg acctggtgga
      841 cgaggcgtgc gcgctcatcc gtacggagat cgactcgctt cccgcggagc ttgacgccgt
      901 ctcgagaaag gtgatgcagc ttgaaataga ggaggccgcg ctcaacaagg agaaggacgc
      961 ggcgagcgtc gaacgtttga aggcgctcca gaaggagctg caggaggcgc gcgaggagca
     1021 gaccggcctg cgcgcgcagt acgaggccga gaagcaggga attggcgttg tccgcgagct
     1081 gcgggagcgc atcgaatcaa tcaggcgcga tatcgagaag gcagagcgcg agtacgacct
     1141 gaacagggcc gccgagctga agcacggcac gcttccacag cttgaggccg aactgcgcga
     1201 gcgggagaaa acgcttgaga acgggcacgc cgggccgaag atcctccgcg aggaggtcac
     1261 cgaggaggag atttccgaga tcgtcagccg ctggacgggc atccccgtca cacgccttct
     1321 tgaaggggag aaggagaagc tgcttcaact cgacgaggtg ttgcatcggc gcgtcgtcgg
     1381 tcaggacgag gccgtcgagc tggtggccga cgcggtgctc cgcgcccgct ccggcatcaa
     1441 ggacccgcgg cggccgatag gatcgttcat ctttctgggg cccacgggcg tcggcaagac
     1501 ggagctggcc aagacgctcg ccgagtcgct cttcgacagc gaggagaatc tgatccggct
     1561 ggatatgtcg gagtacatgg aaaagcactc cgtctcccga atggtgggag cgcctccggg
     1621 gtacgtcggg tacgacgagg gggggcagct gaccgaggcg gtgcgccgca ggccctattc
     1681 cgtcatcctc ttcgacgaga tcgaaaaggc gcatcccgac gtcttcaaca tcctcctgca
     1741 gatactggac gacgggcgca tcaccgacag tcaggggcat cttgtcaact tcaagaacac
     1801 tgtggtcatc atgaccagca acatcggcgc gcagtatctg ctcgaaggga tcacctccga
     1861 cgggcagatc cgcgagagcg cgcggacgca ggtgatggac gagctgcgcc gcgccttccg
     1921 gccggagttc ctgaaccgcg tgga
//
LOCUS       DAOPKU010000574         1943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_69154_length_1943_cov_3.266419, whole genome shotgun sequence.
ACCESSION   DAOPKU010000574 DAOPKU010000000
VERSION     DAOPKU010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69154_length_1943_cov_3.266419"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PK849_13755"
     CDS             complement(<1..753)
                     /locus_tag="PK849_13755"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPE67241.1"
                     /translation="MDAIRRMIKDRCMEKEEPFCASACPFHLDVREFIARMQRGAFNT
                     AFRLFSNTTGFPAIVAAHCHEPCAAVCPRGTVDAPVQLNLLEKAAVAYAANTKPNSYN
                     LPPKKGRIAVVGAGLSGLGCALRLANRKYRVDVYERSGRVGGRLWDEMDPEIFLADIR
                     LQFSNEEYGLFLDHTVETLEPLRAQYDAVYVATGNGGDAFGLTCENDVREGIPWASRL
                     PGVFLGGALLGANSMEALSQGLKASLCIEDYLK"
     gene            complement(825..1889)
                     /locus_tag="PK849_13760"
     CDS             complement(825..1889)
                     /locus_tag="PK849_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE67242.1"
                     /translation="MPEKRRGVGISWGGFNVTEGGTDKATVAIELNPDGTFTKYDTYQ
                     ELGQGGDIGSLMLTLEALKPLGVTPEQIKQVQNDTKLCPDAGMSGASRTHFMSGNATI
                     IAAGKLIAAMKKPDGTYRTYEEMKAEGLETKYFGAYSNTSVPGLCRLDPNTGIGDPTP
                     AFTYCLNLAEVEVDTSTGKTSVLRFTCVDDVGKIGNIDAVNGQAYGGISHAIGFALGE
                     NYDDVKKHTNIAASGVPYIETIPDEIEVIHYERPDEAGPFGSSGASEGFQSSGHMAVL
                     NAINNACGVRVYEMPATPEKVKAGLALLASGGTIEPPKKYFLGSDLYEELENIKANPV
                     QVGPEDYFVPLGGASAERFF"
BASE COUNT          318 a          597 c          608 g          420 t
ORIGIN      
        1 cttcagatag tcctcgatgc agagcgaggc cttcagtccc tggctgagcg cttccatcga
       61 attcgctcct aagagcgcgc cgccgaggaa gactccgggt agacgactcg cccatggtat
      121 gccttcgcgc acgtcgtttt cgcaggtcag gccgaaagcg tcgcccccgt tccccgtcgc
      181 gacatagact gcgtcgtact gtgcacgcag cggttccagc gtttcgaccg tgtggtcgag
      241 gaagaggccg tactcttcgt tcgagaactg gaggcgtata tcggcgagga agatctccgg
      301 atccatctcg tcccaaaggc gtccgccgac gcggccgctt cgctcgtaga cgtccacacg
      361 gtatttcctg ttcgccagcc gcagcgcgca ccccagaccg ctcagtcccg ccccgaccac
      421 ggcgatcctc cccttcttcg gggggaggtt gtagctgttc ggcttggtat tcgccgcata
      481 cgcgacggcc gctttttcga ggagattgag ctgtacggga gcgtcgaccg ttcctctcgg
      541 acagaccgcg gcgcacggct cgtggcagtg cgccgcgacg atcgcgggaa agccggttgt
      601 gttcgagaag agacggaatg cggtgttgaa agcgccccgc tgcatccgcg cgatgaattc
      661 gcgcacgtcc agatggaacg gacaggccga ggcgcagaaa ggctcctcct tctccatgca
      721 gcgatccttt atcatgcgtc tgattgcatc catcgtccaa tcccctccgg agatgccggg
      781 agcccgtatc ttcagcagat acaggctccc atgaacatgt tttgttagaa gaagcgctcc
      841 gcggaggcgc cgcccagagg aacgaagtag tcctcgggcc cgacctggac cgggttggcc
      901 ttgatgttct caagttcctc gtagagatcc gagccgagga agtacttctt cggcggctcg
      961 atggtcccgc ccgaagccag cagagcaagc ccggccttga ctttttcggg agtggcgggc
     1021 atttcgtaga cgcggacgcc gcacgcatta ttgatcgcgt tgagcaccgc catgtgcccg
     1081 gaggactgga agccctcgga tgcgcccgaa gaaccgaagg ggccggcttc gtccggacgc
     1141 tcgtagtgga tgacttcgat ctcgtccgga atggtctcta tgtaggggac gccgctcgcg
     1201 gcaatgttgg tgtgtttttt tacgtcgtcg tagttctcgc cgagcgcgaa gccgatcgcg
     1261 tgcgagatgc cgccgtacgc ctgtccgttg acggcgtcga tgtttcctat tttccccacg
     1321 tcgtcgacgc acgtaaagcg cagcacgctc gtcttcccgg tggaagtgtc gacctcgact
     1381 tcggccaagt taaggcaata ggtaaaagcg ggcgtcggat cgccgatgcc ggtgttcggg
     1441 tcgagtctgc acaaacccgg gaccgaagtg ttcgagtagg cgccgaagta cttcgtctcc
     1501 agcccttcgg ccttcatctc ctcgtaggtt ctgtaggttc cgtcaggttt cttcatggcg
     1561 gcgatgagct tcccggcggc gatgatggtg gcgttgccgc tcatgaagtg cgtgcggctc
     1621 gccccggaca tccccgcatc cgggcagagt ttcgtgtcgt tctgtacctg cttgatctgt
     1681 tcgggggtga cgccgagagg tttcagcgcc tccagcgtga gcatcaggga gccgatgtcg
     1741 ccgccctgac cgagctcctg gtaggtgtcg tactttgtga acgtgccgtc cgggttcagt
     1801 tcgatggcga ccgtggcctt gtcggtgccg ccctcggtga cgttgaatcc cccccaggag
     1861 atgccaacgc ctcggcgctt ttcgggcatc ggcggctttc gcctcggcga cggcgcgctc
     1921 gtagtgcggt cgcatttttt tca
//
LOCUS       DAOPKU010000575         1942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_69176_length_1942_cov_6.096449, whole genome shotgun sequence.
ACCESSION   DAOPKU010000575 DAOPKU010000000
VERSION     DAOPKU010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69176_length_1942_cov_6.096449"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1598)
                     /gene="ltrA"
                     /locus_tag="PK849_13765"
     CDS             complement(114..1598)
                     /gene="ltrA"
                     /locus_tag="PK849_13765"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HPE67243.1"
                     /translation="MEHGRPLRSGVAPRGKEYKAEAEISDTDRRGVGGVHSIDDGADN
                     TTRPREGTLLHPRLHERTGDLLHELSGSEEKARAFQRNLYRKAKREKEFRFYSLYDKI
                     WRRDILEYAWKRCRANGGSPGIDGKSFKAIEEEGVGRLLEEIARELREKTYRPSPVKR
                     VYIPKADGSKRPLGIPTIKDRVVQTACLIVVEPIFEADFEDISYGFRPKRSAHGAIEE
                     IVEHVERGFTAAYDADLTKCFDTIPHDVIMEGLKRRVSDGNVLSLVLKFLRTPVVEPD
                     GPVQGTKNRTGTPQGGVISPLLANIVLDALDKKGAELKKLYNARMVRYADDFVILARY
                     IGDPILKAVEETIAGLGLKINEEKTRIVRLWNGETLNFLGYAMNMVGKSGRVALRPSE
                     KAQKKLKERLREIISRQRLYKGIDGIVKELNPLLTGWKNYFRLTTVKREFHDLDFHII
                     GRFHRVAKKSSQRPSRIFRHGVHATLKFMGLRSLCGDRPVNAFR"
     gene            complement(1681..>1942)
                     /locus_tag="PK849_13770"
     CDS             complement(1681..>1942)
                     /locus_tag="PK849_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67244.1"
                     /translation="GFQGPAKVEVRREKSYANQKGASPFRGISRSPWNRRLACRGDPK
                     RQASRKRLLNESGEDAGRSASEPVVASKTQLSSAIPPQQGEA"
BASE COUNT          287 a          624 c          527 g          504 t
ORIGIN      
        1 cgtgtccgcc tttcggcttt gtggcaaaga acgcccggac cgtgcgacgc tcgccttctc
       61 cccctcgtcc aaccgtacgt gcggttttcc cgcatacggc tgcccgacat ccttcaccga
      121 aaagcgttca cgggcctgtc tccgcacaag cttcgcaatc ccatgaattt cagggtcgcg
      181 tggactccgt gcctgaatat ccggctcggg cgttgactgc tcttcttcgc cactctgtgg
      241 aatctgccga tgatgtggaa gtccagatcg tggaattccc gtttcaccgt ggtcagcctg
      301 aagtagttct tccagccggt caacagaggg ttgagttcct tcacgattcc gtcgatgcct
      361 ttgtacagtc gttgtctgga gatgatttcc cggagtcgtt ccttgagttt cttctgcgct
      421 ttctccgacg gtcgcagggc gacgcggccg ctcttcccga ccatgttcat ggcgtagccg
      481 agaaaattca gcgtctcccc gttccagagc ctgacgatgc gcgtcttctc ctcgttgatc
      541 ttcagcccga ggccggcgat ggtttcttcg acggctttca gtatcggatc tccgatgtag
      601 cgcgcgagta tgacgaagtc gtcggcgtag cggaccatcc gggcgttgta gagcttcttg
      661 agttccgcgc ccttcttgtc cagcgcgtcg agcacgatgt tggcgagcag gggcgatatc
      721 acgcccccct gcggggttcc cgtgcggttc ttcgttcctt gaaccggtcc gtcgggttcc
      781 accacgggtg tcctgagaaa tttcaggacg agagagagca cgttgccgtc cgatacgcgt
      841 cttttcaggc cttccatgat gacgtcgtgc gggatggtgt cgaagcattt cgtcaggtcc
      901 gcgtcgtagg cggccgtgaa tccccgctcc acgtgttcga ctatctcctc tatggctccg
      961 tgagcgctcc tcttggggcg gaatccgtag gaaatgtctt cgaagtccgc ttcgaagatc
     1021 ggctcgacga ctatgaggca cgccgtctgc accactctgt ccttgatcgt cggtatcccc
     1081 agcggacgtt tgcttccgtc cgccttcggt atgtacactc tcttcaccgg cgaggggcga
     1141 taggtctttt cccggagttc gcgcgctatc tcttcgagga gtcgccccac accctcctcc
     1201 tcgatggcct tgaagctctt cccgtcgatt cccgggcttc ctccgttcgc tctgcatcgt
     1261 ttccaggcgt attccagtat gtcgcgtctc catatcttgt cgtacaggct gtagaatcga
     1321 aactcctttt cccgcttggc ttttcggtac aggttgcgct ggaaggcgcg ggccttttct
     1381 tccgatcctg atagctcgtg caatagatct cccgtccttt catgcaatcg tggatgaagc
     1441 agcgtccctt ctctcgggcg ggttgtgttg tccgcgccgt cgtcgatact atggacgcct
     1501 ccgactcctc tccggtcggt atcggaaatt tcggcctccg ccttatattc cttccctcgg
     1561 ggcgcaaccc cggaccggag aggtctcccg tgttccatac gcgctccttc gatgcgtgcc
     1621 gcccctaatc accacggccg tcccgatgtc tgcttcttcc cgttgcttcg tacatcggtg
     1681 tcaggcttct ccctgttgag gcgggatcgc cgacgacaac tgcgttttcg aggccaccac
     1741 gggttcgctt gcgctgcggc ccgcatcttc acccgactcg ttcaagagcc ttttccggct
     1801 tgcttgacgc ttcgggtcgc ccctgcacgc caaccggcgg ttccacggtg aacgggatat
     1861 tccgcggaag ggactcgcac ccttctggtt cgcatatgac ttttcacggc gcacctcaac
     1921 tttcgcagga ccttgaaaac cg
//
LOCUS       DAOPKU010000576         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_69249_length_1941_cov_3.507953, whole genome shotgun sequence.
ACCESSION   DAOPKU010000576 DAOPKU010000000
VERSION     DAOPKU010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69249_length_1941_cov_3.507953"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="PK849_13775"
     CDS             <1..1599
                     /locus_tag="PK849_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPE67245.1"
                     /translation="VLVFILWPTVAILKESVLIDGAFSLERYRDLLTDNRTLLKNSLF
                     VGWWTAVLAVLIGLCCALYVTRTAFRGKRAVLGVLLMTMIAPPFVSSLSYIMLFGRRG
                     LVTWRLLGLRWNPYGAHGVVLMESVGLAAVAAFLIMAVLRGIDRSLEQASFDLGAGRW
                     ETLLRVTLPLARPGVVTAALIVFIRSLSDFGTPMFIGGNYNVLATQAYMTAMGSFDLP
                     KAAAISTLLVIPAFLVFLVYRELMAGAPLFSSKAVAAGEERPLPGAVRWVVFAVVWSF
                     VLFEAMKYGAIFCGAFVRTWGVDFSFTLHHLKTLQLTKLDSLLRSVRYAFAAALAAGA
                     MGVVLARFLGRRSTLFSRAVDFIADLPFILPGPFFGIAYLLAFNRLPEAFLGTGFLIV
                     ANCVYRQLSLGIKSGVSVLGQINPELEDAVRDQGGGGLAVLRDVIGPLLAPAFLVSFI
                     NTFTFTMTTIGGIIFLITPYTKVLTAEMFDAIQSGDIGASSVMASVIILVAMTVNVTF
                     SWLFLKRRTKGSEAEYVSSVGAAR"
     gene            1571..>1941
                     /locus_tag="PK849_13780"
     CDS             1571..>1941
                     /locus_tag="PK849_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE67246.1"
                     /translation="MYLRLERLDKKFEATEAVRELSLDIERGEMLSLLGPSGCGKTTT
                     LRMVGGFLRPDGGRIVLDGEDITALPPERRPTATVFQSYALFPHMSVLRNVIYGLKFR
                     KVPREEALSAGRAMLEKVGLPG"
BASE COUNT          282 a          620 c          627 g          412 t
ORIGIN      
        1 gtacttgttt ttatcctctg gccgacggtc gccattctga aggagagcgt cctgatcgac
       61 ggcgcgttca gcctggagcg ctaccgcgac ctgctgacgg acaaccggac gctgctgaag
      121 aacagcctct tcgtcggatg gtggacggcc gtccttgcgg tgctgatcgg cctgtgttgc
      181 gccctctacg tgacgcgcac cgcgtttcgg gggaagcggg cggtgctggg ggttctcctg
      241 atgacgatga tcgcccctcc cttcgtctcg tccctctcgt acatcatgct cttcggacgg
      301 agagggctcg tgacctggcg gcttctgggg cttcgctgga acccgtacgg agcgcacggc
      361 gtcgttctga tggagtccgt cgggctggcc gccgtcgccg cgttcctgat aatggcggtg
      421 ctccggggca tcgacagaag tcttgaacag gcctcgttcg acctcggcgc gggaaggtgg
      481 gagacgctgc tccgcgtcac gcttccgctc gcccgcccgg gcgtcgtcac ggcggcgctg
      541 atcgtcttca ttcgctcgct ctccgacttc ggcacgccga tgttcatcgg cggcaactac
      601 aacgtgctcg cgacgcaggc gtacatgacc gcgatgggct ccttcgacct tccgaaggcc
      661 gccgccatct ccacgcttct cgtcatccca gcctttctcg tctttctggt ctaccgagag
      721 ctgatggccg gggcgcccct cttttcttcc aaggcggtcg ccgccggaga ggaacgcccg
      781 cttcccggag cggtgcgatg ggtcgttttc gcggtcgtct ggagcttcgt cctcttcgag
      841 gccatgaagt acggcgcgat cttctgcggc gccttcgtca ggacgtgggg cgtcgatttt
      901 tcctttacgc tccatcattt gaagacgctc cagctcacca agctcgacag cctgctccgg
      961 agcgtccgct acgccttcgc cgccgccttg gccgcgggcg cgatgggcgt cgtcctggcg
     1021 cgctttctcg ggaggcgctc cacgctgttc agccgggccg tcgatttcat cgcggatctc
     1081 cccttcattc tgccggggcc gttcttcggc atcgcctacc ttctggcgtt caaccggctg
     1141 cccgaggcgt tcctggggac ggggtttctc atcgtcgcca actgcgtcta ccggcagctc
     1201 tcgctcggga tcaagagcgg cgtctccgtg ctgggacaga tcaacccgga gctcgaggac
     1261 gccgtccgcg atcagggagg aggaggcctc gccgtgctgc gggacgtgat cgggccgctg
     1321 ctcgcgccgg cgttcctggt gagcttcatc aacaccttca cgttcaccat gaccaccatc
     1381 ggcggcatca tctttctcat cacgccgtac acgaaggtgc tcaccgcgga gatgttcgac
     1441 gctatccaga gcggcgatat cggcgcgagt tcggtgatgg cgtcggtgat catcctggtc
     1501 gccatgacgg tgaacgtgac gttttcgtgg ctcttcctga agaggagaac aaaaggatcg
     1561 gaggcggagt atgtatcttc ggttggagcg gctcgataaa aaattcgaag ctacggaggc
     1621 ggtacgggag ctctctctcg atattgaacg cggggagatg ctctcgcttt tggggccgag
     1681 cggatgcggc aagacaacga cgctgcggat ggtcggaggc tttctccggc ccgacggcgg
     1741 gcggatcgtc ctcgacgggg aggatatcac cgcgcttccg ccggagcggc gccctacggc
     1801 gaccgtcttc cagagctacg cgctcttccc gcacatgagc gtgctgcgga acgtgatcta
     1861 cggcctgaaa ttccgcaaag ttccccgcga agaggccctc tcggcgggga gggcgatgct
     1921 tgagaaggtc ggccttccgg g
//
LOCUS       DAOPKU010000577         1934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_69586_length_1934_cov_4.199042, whole genome shotgun sequence.
ACCESSION   DAOPKU010000577 DAOPKU010000000
VERSION     DAOPKU010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69586_length_1934_cov_4.199042"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PK849_13785"
     CDS             <1..552
                     /locus_tag="PK849_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPE67247.1"
                     /translation="DAFESVKEVAGMLNGLLERGGLTGMLGTVALILVALVLGGIMET
                     CGFLEVLLVRLMQSVKSVFGLVASVLVSCVFTNMFLGDQYLALVMPGRMFKPAFEKFE
                     QNGKKLDPSLLSRSLEDSGTLTSVLVPWNTCGAYNSGVLGVPTLSYLPYAFLNYLNPL
                     VALLMTRLGIGIPWIEAGEKKAE"
     gene            555..995
                     /locus_tag="PK849_13790"
     CDS             555..995
                     /locus_tag="PK849_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67248.1"
                     /translation="MDNVLRGSGTHFPRWMRWTGMGTGIFLLADGMRRLFAGGGDLSG
                     VFAYILIGTACIYIAGYEKKIFLSEKGFEREILFWGRGRKEQVLWMDMEELILVPSGK
                     GTAVLIPLKQRGWRAFFPGIREDDVRKFVARFREDLPVRKGSGS"
     gene            1073..>1934
                     /gene="tal"
                     /locus_tag="PK849_13795"
     CDS             1073..>1934
                     /gene="tal"
                     /locus_tag="PK849_13795"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HPE67249.1"
                     /translation="MVQQNRILQALALGQSMWCDFLSRDLLRSGGLDRMIEQGVRGVT
                     TNPSIFENAIARTSDYDTDIQAMMREKKTEEEIYTALTIADVQEGADRFKTLFETSNG
                     VDGFVSLEVSPLLCHDEKGTVEEALRLFEQLDRKNVMIKIPATSAGMKAIRRCISLGV
                     NVNATLIFSRAQYRQVAEAWLSGLEERAAAGESVKGIASVASLFVSRVDTAVDKLLET
                     TGEKDLAGKIAVDNARAAYSDFEAIAASARWKTLAEKGALLQRPLWASTGTKNRAYSP
                     TLYVDSLIGPH"
BASE COUNT          448 a          466 c          547 g          473 t
ORIGIN      
        1 gatgcgttcg aatctgtcaa ggaggttgca ggcatgctga atggtcttct cgaaagagga
       61 ggtttaactg gcatgcttgg gacggtggcg ctcatcctcg tcgctttggt tcttggcggt
      121 attatggaga cgtgcggttt ccttgaagtc cttttggttc gcttgatgca gagcgtcaaa
      181 tcggtgttcg gtcttgtcgc ttcagttctt gtatcgtgcg tcttcactaa catgttcctt
      241 ggcgaccaat accttgcgct tgtcatgccc ggacgaatgt tcaaacccgc ctttgaaaaa
      301 ttcgaacaga acgggaaaaa acttgatccg agcctgctct ccagatcgct tgaggattcc
      361 ggaacgctca cctcggttct tgttccgtgg aatacgtgcg gcgcctataa tagcggtgtc
      421 cttggcgtgc ccactctgtc atatcttccg tacgcattcc tgaattacct gaatcctctt
      481 gttgctctgt tgatgacaag gctcggtatc ggtatacctt ggattgaagc cggcgaaaag
      541 aaggctgaat aggcgtggac aacgtgcttc gggggagcgg aacgcatttc ccccgctgga
      601 tgcggtggac tggaatgggg acgggcatct ttctcctggc agacggaatg cgccgtctct
      661 ttgccggcgg cggcgatctc tcgggtgttt ttgcctatat tctgatcggc acagcgtgta
      721 tctacattgc cggatacgaa aagaagatct tcctctcaga gaaggggttt gagagagaga
      781 ttcttttctg gggacggggc cgcaaagaac aggttctctg gatggatatg gaggaactca
      841 tcctcgttcc atcggggaag ggaaccgccg ttcttatccc cctgaaacag agagggtgga
      901 gggctttttt cccgggaatt cgcgaggatg atgtgagaaa attcgtagct cgcttcagag
      961 aagaccttcc cgttcggaaa gggagcggtt cataagaaaa ttgtgaacgg cgcggaggag
     1021 gagacttccc gtctcctcca atttctccac tctctatgaa aggatagatt ccatggttca
     1081 acagaatagg atattgcagg cgctggcgct tggacagagc atgtggtgcg acttcctgag
     1141 cagagatctg ctccgttccg gaggtcttga caggatgatc gaacaaggag tgcggggagt
     1201 aacaacgaat ccctccatct tcgagaacgc aatcgccagg acatccgatt acgacacgga
     1261 tattcaagcg atgatgcgcg agaaaaaaac ggaagaggag atctatactg cactcaccat
     1321 cgccgatgtg caggaaggag ccgaccgttt caagacgctt ttcgagacaa gcaacggggt
     1381 tgacggtttt gtaagtcttg aggtaagtcc tttgctctgt cacgatgaaa aaggaactgt
     1441 tgaagaagcc ttgcgtctct tcgaacagct tgacaggaaa aacgtcatga tcaagatacc
     1501 ggcgacatct gccggtatga aagccattcg tcgctgtatc tctctgggag tgaacgtcaa
     1561 cgcaacgctc attttttccc gggcgcagta ccgacaggtt gcagaagcat ggctttcggg
     1621 tcttgaagaa agagctgcgg caggcgaatc ggtaaagggc atcgcctcgg ttgcctctct
     1681 ttttgtcagt cgagttgaca ccgcggtgga caagcttctc gaaacaacgg gagaaaagga
     1741 tcttgccggt aaaatcgcag tcgacaacgc ccgtgccgca tattccgact ttgaagcgat
     1801 agcggcatcc gcacgatgga aaactcttgc ggaaaagggt gctttgcttc agcgcccatt
     1861 atgggccagc acgggaacga aaaaccgcgc ctactccccg acgctctacg ttgactctct
     1921 tataggtcct cata
//
LOCUS       DAOPKU010000578         1927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_69951_length_1927_cov_5.292735, whole genome shotgun sequence.
ACCESSION   DAOPKU010000578 DAOPKU010000000
VERSION     DAOPKU010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69951_length_1927_cov_5.292735"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK849_13800"
     CDS             complement(<1..366)
                     /locus_tag="PK849_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPE67250.1"
                     /translation="MFEAEYSIPYAGIGPDGAMKLPVLLDILQDMADRDAHRMSMTVA
                     DLLPRGLSWVLRQYRVVVDRYPGHESMLKVRTWHGPRRNLHSIRAFEVRDAAGPVASA
                     WTSWILIDTLRGRPLRLDRH"
     gene            557..1618
                     /locus_tag="PK849_13805"
     CDS             557..1618
                     /locus_tag="PK849_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPE67251.1"
                     /translation="MRRILFLTLLLVITAAPSVIAEQPPFGVYTVKAGDTVDSVAGRY
                     GVDPANLASANGIAANDGLPPEGTVLLVPKSPGDVLATLYEAKRRGLGAWPKPKYADE
                     FLPPLTEEPPSDKKPSAPKERPQKAEHTPAPAVSSQQPAAPADGTARTHKVQEGETMY
                     GISRLYGLSLSALLQANNLTESSIIKIGETLTIPGTSAEKPEPHVQRPTPPQNEPIVG
                     KTPAEAPPSPTTPMRLTWPLRGGPPPTHTGTSFGEGLSAKAAPGEPVHAAADATVLHG
                     GWMRNFGNAIYLNHGNGIATFYGGLGIIYVKSGEKIRRGTKIALVGEIQGAEPRFVFH
                     VLKEGKSVDPRPWIEGAKQ"
BASE COUNT          441 a          598 c          521 g          367 t
ORIGIN      
        1 gtgccggtca agcctgaggg gacgtccgcg cagagtatcg ataaggatcc agcttgtcca
       61 tgcggacgca acgggtccgg cggcgtcgcg aacctcgaaa gcgcggatcg aatgcagatt
      121 tctccgcggt ccgtgccacg ttctgacctt taacatactc tcgtgcccgg gatagcggtc
      181 cacgacgacc ctgtactgac gcagcaccca gctcaaccct ctcggcagaa gatcggccac
      241 ggtcatggac atcctgtgcg catcccggtc ggccatgtcc tgcaatatat cgagaaggac
      301 gggaagcttc atggccccgt ccggtccaat ccccgcgtag ggtatggagt attcggcttc
      361 aaacacgtaa tcatctcgtt tctctggaat ataatatgcg aagggaacag gacggaacgt
      421 gctgcatctt ttccccagcg tcgctttttc gaggtatatt atagtacaga acgagtttct
      481 tctctcccgt gcggaagaac aggctcccgc cgggcgaaca tcatgtcctt cgggacgcca
      541 ggaggttgaa cagagcatga gacgcatact cttcctcaca cttcttctcg ttattacggc
      601 cgcgccttcc gttatcgcgg aacagccccc gttcggcgtc tacacggtaa aagccggaga
      661 caccgtagac tcggtagccg ggaggtacgg cgtcgacccc gcgaacctcg cctcagccaa
      721 cgggatagct gcgaacgatg gtcttccacc ggaggggacg gtgctgctgg ttcccaaatc
      781 accgggcgac gttcttgcca ccctctacga ggcgaaacgc cgcggcctgg gagcatggcc
      841 gaagccgaaa tacgccgatg agtttctgcc gcccttgaca gaagaaccgc cctcggacaa
      901 gaagccttcg gccccgaagg agcgcccgca gaaagccgaa cacacacccg ctccggcagt
      961 atcttctcaa cagcctgcgg ctccggcgga cggaacagca cgcacccata aagtacagga
     1021 gggagagacc atgtacggaa tttcgcgtct ctacggcctc tcgctgtccg cgctgctgca
     1081 agccaacaat cttaccgaaa gttccattat caagataggc gagacgctca cgattcccgg
     1141 aacctcggcc gaaaagccgg aacctcatgt gcagcgcccg accccgccgc agaacgagcc
     1201 gatcgtcggg aagacgcctg cagaagcgcc tccatcacct accacaccga tgcgcctgac
     1261 ctggcctctc cgtggaggtc ctccccccac gcataccgga acgagcttcg gagaagggct
     1321 ttccgcaaag gctgcccccg gcgaaccggt tcatgcggcg gcggatgcga cagtccttca
     1381 cggcggatgg atgcgaaatt tcggcaacgc gatctatctg aaccacggca atggtattgc
     1441 cactttttac ggaggactcg ggatcatcta cgtaaaatcc ggagagaaga tacggcgtgg
     1501 aacaaagatc gcccttgtcg gagagattca gggagccgag ccgaggtttg tcttccatgt
     1561 cttaaaggaa ggaaaatccg tcgatcccag accctggata gaaggcgcaa aacagtaacc
     1621 gtttttcaga cgcggacctc acagcgcggg ggagagtgtc gccggacgac ttccccccga
     1681 cgctgcaagt ccgccctccg ctcagtaacg aagtagctgc tccataagaa ccgccctcgg
     1741 tgtcattcat ttcctcaagt catctggaca aaaagcgaga gtaataacac atcctccggc
     1801 gaaaaatacc cgtgaatcca cagagaaaaa gtgagcagct caaatactcc gctgcattct
     1861 cttctttcgg catgattggc ttttgccccg atgctttccc ccggcactga agatcgtgtg
     1921 ctctttt
//
LOCUS       DAOPKU010000579         1925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_70057_length_1925_cov_5.776471, whole genome shotgun sequence.
ACCESSION   DAOPKU010000579 DAOPKU010000000
VERSION     DAOPKU010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70057_length_1925_cov_5.776471"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PK849_13810"
     CDS             complement(<1..1401)
                     /locus_tag="PK849_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE67252.1"
                     /translation="MTSFNSRFSRVHRIRKSLGIIFFFSFFYSLFSSSPVPSQGFYMV
                     GISSFSSASASARVVRYGMVELSPFFFKDENGNFTGYMPDLLRTLAENEGWRLSFINA
                     GGEELVKMLQEGEIDLLSMAPTPRLEGLFDFSKNHHYSTWYTFFTRSDSEILTFQDLE
                     GKTISMQSGFYALSELRRILDGIGIRYTIAEAPTVEEALEMLRSGVADVCAAEQLASL
                     AKIRRFDFRRSPIIFAPSKIFFTTTKGKNTALLARLDEKLSELNESPVPLMEVLLRRW
                     YYDDEFTFFPQWALWSFFAASILLALLSAGFVIFYKKGRELRLQGVRLQERLDSERFL
                     SDAARMLLTEGGHEDVLRNACRKIREVIDARRILLLRSTFSSTRRRSIEICFEYASPG
                     LPLLSEHSEVRSVLISDIPRKWLIDLTRKKVVKERLYETPLKKFFSVSEGAFFSFYPL
                     FEGGRFWGALAVNIDDT"
BASE COUNT          570 a          425 c          440 g          490 t
ORIGIN      
        1 tgtatcgtct atgttcacgg ctaatgctcc ccagaaacgt cccccttcaa aaagcggata
       61 gaaagagaag aatgcccctt ccgaaacgga gaagaatttt ttcaatggag tttcgtagag
      121 ccgctctttt acaaccttct ttcgtgtaag gtcaatgagc cactttctag gaatatcgct
      181 tatcaggacg ctccgcactt cagagtgttc cgaaagaagc ggtagtccgg gtgatgcata
      241 ttcaaagcat atttcgatcg aacgtcggcg tgtagaagaa aaagtgcttc ggaggagaag
      301 aattctcctg gcatcgatca cttcacggat ttttcggcac gcgttgcgca gaacatcttc
      361 atgcccgcct tccgtaagta gcatacgcgc ggcgtcacta agaaaacgtt ccgaatcaag
      421 tctttcttga agccgcaccc cctgtaaacg aagttcacga ccctttttat agaagataac
      481 gaatccggct gaaagtaatg ccaagagaat tgacgctgca aagaagctcc acaatgccca
      541 ttgagggaag aaggtaaact catcgtcgta gtaccatcgg cgtaaaagga cttccatgag
      601 aggtactgga gactcgttca gttcggaaag tttttcgtcg agtcgagcca agagcgctgt
      661 gttttttcct tttgtagtgg taaaaaagat tttggagggg gcaaaaataa tcgggcttct
      721 ccggaaatca aaacgtcgaa tcttggccag gctagcaagc tgttccgccg cgcatacgtc
      781 ggcaacgccg gagcgcagca tctcaagcgc ttcctccact gttggcgctt ccgctatcgt
      841 gtagcggatt cctataccgt ccaggattcg gcggagttcg gaaagcgcat aaaagccgct
      901 ttgcattgaa atagttttgc cttcaagatc ctgaaatgtc agtatctccg agtcagaacg
      961 cgtgaaaaag gtgtaccacg tagaataatg atgattctta gagaaatcga aaagcccttc
     1021 caaacggggc gtaggagcca tactcaaaag gtcgatttct ccctcctgaa gcattttgac
     1081 aagctcttca ccgccggcgt taatgaagga aagcctccac ccctcatttt cagcaagggt
     1141 tcgcagaaga tccggcatat agcccgtaaa gttcccattc tcatccttga aaaaaaacgg
     1201 ggataactct accattccgt atcgaacaac acgcgcggaa gcggaagcag aagaaaacga
     1261 agaaatgccc accatataaa aaccctggga aggaacgggg gaagaagaaa aaagactgta
     1321 aaaaaaggaa aaaaagaaga taatgccaag agatttcctg atacggtgta ctcttgaaaa
     1381 acgagagttg aatgaggtca agaaacagac actccctttc ggagtaaaac aaccttgcca
     1441 tgttttctca atatttattt cggacccgca atggttttaa aatatgtatt ccatgtcatc
     1501 gctgtacaaa gaaagcaaat agactgcttg agaatatttt gataacgcat cgcaggctca
     1561 tctgaaagaa ttatatgaag aaaacaagag ttgcgcaagc ccaattccag gcttacgttg
     1621 tctattaagt gggcactaag gttcatatat ataaaagttt atctcgtttt ttgtcgctac
     1681 gtgtactaaa agtatctttt gacattactt tgattgtggg gtggattctt ggagtgagaa
     1741 cgaaaaaaaa tggaagccct aatagcggtt aagctcgatt gcatcaagtt cctgcgagac
     1801 tacctccgct ttattcagag gttccgtcta tcatcatctg tctttccttc ttgaaaacca
     1861 tggaggttcc agggaggaat aacgctcttt catttttttc gttttcttaa aacatatggt
     1921 tgtgg
//
LOCUS       DAOPKU010000580         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_70388_length_1919_cov_3.086373, whole genome shotgun sequence.
ACCESSION   DAOPKU010000580 DAOPKU010000000
VERSION     DAOPKU010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70388_length_1919_cov_3.086373"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PK849_13815"
     CDS             complement(<1..807)
                     /locus_tag="PK849_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPE67253.1"
                     /translation="MLKKLGIMALAVFLMAGATFASDLEPSGKGMRVWFDAGGSAGEP
                     YSTILINGAKQAAADLGVELTIVYSDWNAEKMLENFKNGLAARPTGYVVIGVPGDDGF
                     APLVDEAFADGMIVTAVDTELPRLQAKYQSRGFGYIGPDGWKQGEDMAKEILRRKEFK
                     PGDRAFVWGLKRLESRGRRARAILEVFEKAGLKVDYLEISDEVNKDAALGAPVLAGYL
                     AANPDCKLIVLDHGALTGQTANFLRGAGLQPGDVYATGFSLNPATAASIES"
     gene            complement(844..>1919)
                     /locus_tag="PK849_13820"
     CDS             complement(844..>1919)
                     /locus_tag="PK849_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HPE67254.1"
                     /translation="EAHEAGTKVIGTYCTYMPWELADAAGAITAVLCAKSDKPIAAAE
                     EQLPRNLCPLIKASYGHALLDTCPYFHFCDAVVAETTCDGKKKMFELMSALKPLHMIR
                     IPHSSSRPEDLEQMKSEFLKTREYIGKVAGRAITDDALRESIALRNRERRALQSLWEL
                     SALDEPPLSGLEVHQFGEYMQYRFDKRKDVPWLEEQVRSIRAAWEGGERREHGKKPRI
                     MITGCPTGGVVKVIEAVEKAGAVVVCYENCSGQKELGYLVDETKDPMEALAEKYLSIG
                     CSIMSPNENRMNSLRALAERYKIDGVVEVLLTACHTYAVESYSVRNLVQSLGKKYLLV
                     ETDYSEGDKEQIATRMDAFVEMM"
BASE COUNT          317 a          652 c          534 g          416 t
ORIGIN      
        1 gctctcgatg gaggccgccg tcgccgggtt caggctgaag ccggtcgcgt agacgtcgcc
       61 cggttgaagc cccgctcctc tgaggaagtt cgcggtctgt cccgtcagcg cgccgtgatc
      121 gagcacgatc agcttacagt ctgggttggc cgcaaggtat ccggcgagca ccggagcgcc
      181 gagcgcggcg tccttattga cctcgtcgga aatctccaga tagtccacct tcagcccggc
      241 cttttcgaag acctccagta tcgcccgcgc cctgcgacct ctgctttcga gacgcttcag
      301 accccagacg aacgccctgt ctccgggctt gaactccttg cgccgaagga tctccttcgc
      361 catatcctcg ccctgcttcc agccgtcggg gccgatgtag ccgaaacctc tggactgata
      421 cttcgcctga aggcgcggaa gttcggtgtc cacagccgtt acgatcatgc cgtcggcgaa
      481 cgcctcgtcg accaaggggg cgaatccgtc gtctcccgga acgcctatca cgacataccc
      541 cgtcgggcgg gcggccaatc cgttcttgaa gttctccagc atcttctccg cgttccagtc
      601 ggagtagacg atcgtcagtt cgactccgag gtcggcggcc gcctgcttcg cgccgttgat
      661 caaaattgtg gagtacggct cgcccgccga accgcccgcg tcgaaccaga cccgcatccc
      721 cttgccgctc ggttcgaggt cggacgcgaa cgtcgcccct gccataagaa agaccgcaag
      781 cgccatgatt cccagttttt tcaacatctc tcttctcctt tcgttttccc gcgaacgcgg
      841 gggttacatc atctcgacga aggcgtccat ccgcgtggcg atctgctcct tgtcgccctc
      901 cgaatagtcc gtctccacga gcaggtactt tttcccgagg gactgcacaa gattccgcac
      961 ggaataggac tccaccgcgt acgtatggca ggcggtgagc agcacctcga ccacgccgtc
     1021 gatcttgtag cgctccgcga gcgcacgcag cgaattcatg cgattctcgt tggggctcat
     1081 gatggagcag cctattgaga ggtacttctc cgcaagcgcc tccatggggt ctttggtctc
     1141 gtccacaagg tagccgagct ccttctgtcc gctgcaattc tcgtaacaga ccacgacggc
     1201 cccggccttc tctacggcct cgatcacctt cacgacgccg cccgtcgggc atcctgtgat
     1261 catgatcctc ggcttctttc cgtgctccct acgctcgccg ccctcccacg cggcgcggat
     1321 gctccgtacc tgttcctcga gccagggaac gtccttccgc ttgtcgaagc gatactgcat
     1381 gtactcgccg aactgatgca cctcaagacc ggaaagagga ggttcgtcca gcgcgctcag
     1441 ctcccagagg gactgcagcg ctcttcgctc tctgtttctg agggcgatgg actcgcgcag
     1501 ggcgtcgtcc gtgatcgcgc gtccggcaac cttcccgata tactccctcg tcttcaaaaa
     1561 ctcgctcttc atctgctcca gatcctcggg gcggctcgaa ctgtgcggaa tgcggatcat
     1621 atgcagcggt ttcagcgcgc tcatcagctc gaacatcttc ttttttccgt cgcaggtggt
     1681 ctccgcgacg acggcgtcgc agaagtgaaa gtacgggcac gtgtcgagca gcgcgtgacc
     1741 gtagctcgcc ttgatgagcg gacagagatt gcgggggagc tgttcctccg ccgccgcgat
     1801 cggtttgtcg ctcttcgcgc agagcaccgc cgttatggct ccggcggcgt ccgcaagctc
     1861 ccagggcatg taggtgcagt atgttcctat caccttcgtt ccggcttcat gcgcctctt
//
LOCUS       DAOPKU010000581         1914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_70624_length_1914_cov_4.376547, whole genome shotgun sequence.
ACCESSION   DAOPKU010000581 DAOPKU010000000
VERSION     DAOPKU010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70624_length_1914_cov_4.376547"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PK849_13825"
     CDS             complement(<1..932)
                     /locus_tag="PK849_13825"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPE67255.1"
                     /translation="MSKVKSAIEAVGLIRDGATVAIGGFVGCMHPEELSNAVEERFLQ
                     SGTPNDLTLVYAAGQGDRKDRGANHFGHPKLLKRVVGGHWGLAPKLQALALENKIEAY
                     NFPQGVISHLFRDIAAKKPGTITHIGLKTFVDPRLEGGKLNARTTEDLVEIVEIGGTE
                     YLRYMPFPIDVALVRGTFCDAAGNATFEKEALALEVVAMCQAAKNSGGIVVLQVEKVL
                     PKSELNAKLVRLPGIYVDAIVEATPERHMQTFGVQYNPSYCGDAKASIGSIEPLAMDE
                     RKIIARRCAMELGKDVVINLGIGMPEGISIVASE"
     gene            1411..>1914
                     /locus_tag="PK849_13830"
     CDS             1411..>1914
                     /locus_tag="PK849_13830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPE67256.1"
                     /translation="MDIQHLKYFVQVIKNQSFTKAAEKLVVTQPMLSRVINQLEEELD
                     VKLIERTSKLFKTTDAGELLYQQALKVLHTHNDIYQYINDLKSAKSGEVLISIPGVLL
                     DMYFPPLLFEFHQKYPNVKLDIIEEGSKLTAQSVLSEQVELGFVMLPVRNASKLTSRI
                     VTTSICHV"
BASE COUNT          483 a          553 c          389 g          489 t
ORIGIN      
        1 tcttcgctgg cgacgatcga tatcccctcg ggcataccta taccgaggtt gatcacaaca
       61 tccttcccga gctccattgc gcaccggcgg gcgataattt tccgttcatc catagccaaa
      121 ggctctatag aaccgattga ggctttcgca tctccgcaat agctggggtt gtactgaacg
      181 ccgaacgttt gcatgtgcct ctccggcgtg gcttctacga tcgcgtcgac ataaattcca
      241 gggagacgga ccagcttggc gttgagttcg ctcttcggaa gcaccttctc cacctgcaga
      301 acgacgatac ctccgctgtt cttcgcggct tggcacatgg ccacgacttc gagcgccagc
      361 gcctcttttt cgaacgtcgc gtttccggcc gcgtcgcaga acgtaccccg taccagggct
      421 acatctattg gaaacggcat gtaccgcaaa tattccgtac cgcctatttc gacgatttcc
      481 accaggtctt ccgttgttct cgcattcaat ttgccgccct caagacgagg gtcaacgaat
      541 gttttcaacc cgatgtgcgt aatcgttccg ggctttttcg ccgcgatgtc cctgaacaga
      601 tgcgatatga ctccctgcgg gaagttgtac gcttcgatct tgttttccag agccagagcc
      661 tgcagcttcg gagcaagccc ccaatgccct cctacgactc gtttgaggag cttcggatgt
      721 ccgaagtgat tcgctcctct gtccttcctg tctccctgcc cggcggcgta cacaagggtc
      781 agatcattcg gcgttccgct ttgtagaaaa cgttcctcca ctgcgttgga aagctcctcg
      841 ggatgcatgc acccgacgaa ccctcctatc gccactgtcg ctccgtccct gatcaaacca
      901 accgcctcga tcgccgattt cactttgctc atacccgcaa cacgctcctt ccgaactctt
      961 tacccctctc acccgtcctt gagagatcgt tttcattcct ctgggacaag gctttctcaa
     1021 gctcccaaca caaagcatat ttcaatatta aatacaatgc gcacaaccaa aagaactttt
     1081 tttcacaaat aacgcaatag ccgaataggc tcagttccca ctcacagctt catagaaaaa
     1141 tccggaaaaa gctcgagaga tgctttttcg gatggcggaa cacacaggtg aaatgaaagc
     1201 gctacatagc aaccggtttt caaaacagct tacgcaaaaa ctctttaaaa taaatgcttt
     1261 tttaattttg catcttgagc atttattttt ttcatgctac aattctatag tctaaactaa
     1321 gtctatgtaa aatactttct tttaaatggt tcaatgccta catgtaatgt attatatagt
     1381 ggctacttac ccaaaagatt ggagcataca atggacattc aacatttaaa atactttgtt
     1441 caagttataa agaaccaaag tttcaccaag gccgccgaga agcttgtggt cacacaacca
     1501 atgctttcaa gagtcatcaa ccagctcgaa gaagaactcg acgtcaaact gatagaacga
     1561 accagcaaac tcttcaaaac gacggacgcc ggagaactct tgtatcagca ggcattgaaa
     1621 gtactgcaca cccacaacga catctatcag tacatcaacg atcttaaatc cgcaaaatcc
     1681 ggcgaagtat tgatcagcat ccccggcgtc ctcctggaca tgtacttccc ccctcttctc
     1741 ttcgagttcc atcaaaaata cccgaatgtc aagctcgaca ttatcgaaga gggctccaag
     1801 cttacggcgc aatcagtctt gtccgagcag gttgaactgg ggttcgtcat gctccctgtc
     1861 cgcaacgcct ctaagttgac aagccgtatt gtcaccacga gcatctgcca tgtc
//
LOCUS       DAOPKU010000582         1910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_70860_length_1910_cov_4.159569, whole genome shotgun sequence.
ACCESSION   DAOPKU010000582 DAOPKU010000000
VERSION     DAOPKU010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70860_length_1910_cov_4.159569"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="thrS"
                     /locus_tag="PK849_13835"
     CDS             <1..1018
                     /gene="thrS"
                     /locus_tag="PK849_13835"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPE67257.1"
                     /translation="ERSLWIQSGHWDHYRDNMYFTEIDERPFAIKPMNCPGGMLVYKS
                     QIRSYRDLPLRMAELGVVHRHERSGVLHGLMRVRCFTQDDAHLYCRPDQVKDEIKGIM
                     DLCRFIYKDVFGFKYTMELSTRPDNSMGSEEQWKIAETALQQALEETGSEYKLNPGDG
                     AFYGPKIDFHLEDCIGRTWQCGTIQLDFQMPEKFDLKYIGDDGKEHRPVMLHRTVLGS
                     LERFFGILIENFAGAFPYWIAPLQARVLPVSDEFVEYGKSVEQTLRSWGVRVETDERD
                     EKLGKKIRDAQTQKVPYMIVIGEKERESGTVAPRERAKGDLGSMSLEQFRKVLDEEFN
                     PLKQ"
     gene            1208..>1910
                     /locus_tag="PK849_13840"
     CDS             1208..>1910
                     /locus_tag="PK849_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE67258.1"
                     /translation="MNKKRIAVLCVALFVLAFAGAGFASTKFVTIVTGSTGGTYYPIG
                     TILANHFNTALMEKGYKWSAQSSGGTVENLDMMQRGEAEMAIAMANITGFAYTGTQTY
                     EGKKIENLRYVMGLWPDVTQFVVSGSSGIKTWADLKGKKVAVGPAASGTEFSSKVLLQ
                     ALAGLTFDDIKAEYVGYSEAAQALQNGQLDAFNAEAGVPVGAVAELYAGRQEVNMLEF
                     SPEDLAKVKAVAPFYA"
BASE COUNT          433 a          529 c          586 g          362 t
ORIGIN      
        1 cgaacgctcg ctctggattc agtccggcca ctgggatcac tacagagaca acatgtactt
       61 caccgagatc gacgagcgtc ccttcgctat caaacctatg aactgccccg gaggcatgct
      121 cgtctacaag agtcagatcc ggagctaccg cgacctgccg ctcaggatgg cggagctcgg
      181 cgtggtgcac cgtcacgagc ggagcggcgt gctccacggg ctgatgcgcg tccgatgctt
      241 cacacaggac gacgcgcacc tctattgccg ccccgaccag gtgaaggacg agatcaaagg
      301 catcatggat ctctgccgct tcatctacaa ggacgtcttc ggcttcaaat acaccatgga
      361 gctctcgacg cgcccggaca attccatggg ctctgaagag cagtggaaga tcgccgagac
      421 ggccctgcag caggcgcttg aggagaccgg atcggagtac aagctcaacc cgggcgacgg
      481 cgcgttctac ggcccgaaga tcgacttcca ccttgaggat tgcatcggca ggacgtggca
      541 gtgcggtacc atccagctcg atttccagat gcccgagaag ttcgatctga agtatatcgg
      601 ggacgacgga aaggaacacc gccccgtcat gctccacaga acggtcctcg gcagcctgga
      661 gcgcttcttc ggcatcctca tcgagaactt cgccggcgcc ttcccgtact ggatcgcgcc
      721 tctgcaggcc cgggtgctcc ccgtctccga cgaattcgtc gagtacggaa agagcgtgga
      781 acagacgctt cgttcatggg gcgtacgcgt tgagaccgac gaacgcgacg agaagctcgg
      841 caagaagatc cgggacgcgc agacgcagaa ggtgccgtac atgatcgtca tcggcgaaaa
      901 agaacgcgag tccggtacgg tcgctccaag agagcgggcg aagggcgatc ttggttccat
      961 gtcgctggag cagttcagga aagtgcttga cgaggagttc aatccgctga agcaataatt
     1021 ttggttcagc atagtcttcc ttgaggaaag aatgttcaga acaggagtcg cttgaaagcc
     1081 gaagcgactc ctgttacaat caacgagtgt tttgttaaca gtgagtgaag atagtatttc
     1141 ggggggtgaa atcaccgaag acgtggggtg cgatgaagta cacaattacg agagaggtgg
     1201 atgaaaaatg aacaaaaaga gaatcgctgt tttgtgtgtc gcgctgtttg ttctggcctt
     1261 tgcaggagcc ggatttgctt cgacgaagtt tgtcaccatc gtcaccggtt ccacgggcgg
     1321 aacgtactat cccatcggta cgatcctcgc caatcatttc aacacggcgt tgatggagaa
     1381 ggggtacaag tggtccgcgc agagctccgg cggcacggtc gagaatctcg atatgatgca
     1441 gcgcggagag gccgagatgg cgatcgccat ggcgaatata accggcttcg cctacaccgg
     1501 gacgcagacc tacgaaggga agaagatcga aaatctccgg tatgtcatgg ggctgtggcc
     1561 ggacgtgacg cagtttgtcg tcagcggctc ctccggcatt aaaacctggg ccgatctcaa
     1621 gggcaagaag gtcgccgtgg gacctgccgc ctccggcacg gagttcagca gcaaggtgct
     1681 tcttcaggcc cttgccggtc tgaccttcga cgatatcaag gccgagtacg tgggatacag
     1741 cgaggcggcc caggcgcttc agaacgggca gctcgacgcg ttcaacgccg aggccggcgt
     1801 ccctgtgggc gcggtcgccg aactctatgc gggccgccag gaagtgaaca tgcttgagtt
     1861 ctcacccgaa gatcttgcga aagtcaaggc ggttgccccc ttctacgcgg
//
LOCUS       DAOPKU010000583         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_71034_length_1907_cov_3.834773, whole genome shotgun sequence.
ACCESSION   DAOPKU010000583 DAOPKU010000000
VERSION     DAOPKU010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71034_length_1907_cov_3.834773"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="PK849_13845"
     CDS             complement(<1..1256)
                     /locus_tag="PK849_13845"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE67259.1"
                     /translation="MGESVREKKARNRSGGSTLGKKGRDLTALALSGELLPPIGRERE
                     CAEVLSTLCRMEKGNPLLLGDAGVGKTAVAEGVACLLASGNAPADLRNASVVEIKLSA
                     FIEAKRFSGGEDFVNSLAEEVLSDNRETIQTVLFVDGLHSLFLDERSWSDWFKPYLEQ
                     TKFRIIGTTTHKALGRTFERNDVFFRYFSPVFIREFDPETAVEALRGKGALLEAYHAV
                     LIPYDTAATAVELSQRYLPGRRLPEKAIDILDSACVLARNTGEVTREHVRAAVAGLAN
                     LPPEAVACSFADICKDLGERLAQRVKGQPEALKKVSRLLKRRTAGFDRRRERPLGSFL
                     FIGPTGTGKTELAKALAEAFFGSVRYMVRLDMSEYSDDASVSRLLGSNPGYIDSERGG
                     LITEAVRKNPFSLFLFDEIEKAAPTV"
     gene            complement(1324..>1907)
                     /locus_tag="PK849_13850"
     CDS             complement(1324..>1907)
                     /locus_tag="PK849_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67260.1"
                     /translation="PCAGNKSGIIASGAWLEPGSVFLVQELLVVRAPELKDEPRRPFL
                     AHPLQEKCLDAGRELAMHADALLEELREGRGFGESVEAEADFFRSKKKYVDAARALVK
                     EFFGTLAAKDLAKSGFKPRFRSGMLGRLSKQFPHDLLMLLDYCDFSLARHPGYVLDET
                     PGNVAAPALAGILASRLVALDGFLFSFRQHEDD"
BASE COUNT          408 a          643 c          490 g          366 t
ORIGIN      
        1 atcacggtgg gcgcggcctt ctctatctca tcgaacaaaa acaacgagaa aggattcttc
       61 cgcactgctt ccgtaatcag gccgcccctt tccgaatcga tataccccgg attggacccc
      121 aaaaggcggc tgacgctcgc atcatccgag tactcgctca tatcgagacg caccatatat
      181 cgcaccgagc caaagaaggc ctcggcgagc gccttggcga gttccgtctt tcccgtaccg
      241 gtgggcccga tgaaaaggaa gctcccgaga ggccgctccc gcctccggtc gaaaccggcg
      301 gtacggcgtt tcaacagcct gctgaccttc ttcaaggcct cgggctgtcc cttcacccgt
      361 tgcgcaaggc gttctccgag gtccttgcag atatccgcga aggaacacgc cacagcctcc
      421 ggaggcaggt tcgccaatcc ggcgaccgcc gcgcgcacat gctcccgcgt cacttccccc
      481 gtgttccgtg ccagtacgca ggccgaatca aggatgtcga tggctttttc cggaagccgc
      541 cgcccgggca ggtagcgctg cgacagttca acagctgtcg cagccgtatc gtaagggatg
      601 agcaccgcgt gatacgcctc aagaagggct cctttgcccc gaagcgcctc cacggctgtt
      661 tcgggatcga actccctgat gaaaacaggg gaaaagtacc ggaagaacac gtcgttcctc
      721 tcgaacgtcc gcccgagggc tttgtgggtc gtcgtcccga ttatgcggaa ctttgtttgt
      781 tcaaggtacg gcttgaacca gtcgctccag gagcgttcat ccaagaacaa cgaatgaagc
      841 ccgtcaacga agagaaccgt ttgtatcgtc tctctgttgt cggacagcac ctcctcggcg
      901 agggagttga cgaaatcctc tccgccgctg aaccttttgg cttcgataaa agccgacaac
      961 ttgatctcca ccacgcttgc gttccgcaga tcggccggcg catttccgga agcaagaaga
     1021 cacgccaccc cctccgccac cgccgtcttc cccacgccgg catcgcccag caacagagga
     1081 ttccccttct ccatgcggca cagcgtcgaa agcacctcgg cgcactctct ttcccgccct
     1141 atcggaggaa gcagttcacc cgacaatgcg agcgcggtca gatcccgccc tttctttccc
     1201 aaggtggaac cgcctgaacg attccgcgct tttttttccc gcacgctttc gcccatgaag
     1261 catcctcctt tccggcgtgg aatgttgaat atctacggcg aacacggaaa cggaacgcgg
     1321 cgcttagtcg tcctcatgct gcctgaaaga aaacagaaag ccgtccagag caacaagccg
     1381 cgacgcgaga atgccagcaa gcgccggagc ggcgacgtta cccggcgttt cgtccagtac
     1441 atacccggga tgcctcgcca aggaaaaatc acagtaatcg agaagcatca ggagatcatg
     1501 cggaaattgc tttgaaagcc ttcccagcat tccgctcctg aatctcggtt tgaagcccga
     1561 ctttgcaaga tcttttgccg ccagcgtacc aaagaactcc ttcaccaaag cgcgcgcggc
     1621 gtccacgtac ttcttcttcg acctaaaaaa gtccgcctcc gcctctacgg actccccgaa
     1681 gccccgcccc tcgcgcagct cttccagcag cgcatccgca tgcatggcaa gctccctgcc
     1741 cgcgtcaagg cacttctcct gcaacggatg cgcgaggaag ggacggcgcg gttcatcttt
     1801 cagctccgga gcgcggacaa ccagcagctc ctgcaccaga aagacgcttc cgggctcgag
     1861 ccatgccccc gacgcaatta tcccggattt gtttcccgcg cacggaa
//
LOCUS       DAOPKU010000584         1895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_71648_length_1895_cov_5.191304, whole genome shotgun sequence.
ACCESSION   DAOPKU010000584 DAOPKU010000000
VERSION     DAOPKU010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71648_length_1895_cov_5.191304"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PK849_13855"
     CDS             <1..262
                     /locus_tag="PK849_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HPE67261.1"
                     /translation="EVEAILHTHPAVSQAVVVGMPHPVNGEVPKAFIQLREGMQVTSR
                     EIIDFCKKHLAHFKVPRSVEFMESFPLSSTGKVLRRMLKQPK"
     gene            complement(317..790)
                     /locus_tag="PK849_13860"
     CDS             complement(317..790)
                     /locus_tag="PK849_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPE67262.1"
                     /translation="MTEKSFNMKDALRYGIHAEIEAKNFYHTWAESVQEEHLKKELSA
                     LSDWESEHEASLMNYYEKLFGAQCPIDPNMVVEPELHVQTKDFGDTTSLLRVASAAYL
                     SEMRAMEFYERLEKESTGETSAMFRKIKDMEKEHMDITKKRYMKIREDVVGFRAF"
     regulatory      900..1110
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1334..>1895
                     /locus_tag="PK849_13865"
     CDS             1334..>1895
                     /locus_tag="PK849_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HPE67263.1"
                     /translation="MRKMSCAVFVFVVCLFGLLSASAFASEHAKPEGKQGILLVAFGT
                     SEPDARSAIDGIVDAVRKAFPDAEVRLSYTSNIIRRKILKEEGLVVDTPPIALAKMQD
                     EGFTSVVVQSLHIISGEEFHQLAGIVRAFSSIRGKYGFERLSLGRPLLDSMEDYRKTV
                     DMLKAKYASFAADGGVVTFMGHGTHHA"
BASE COUNT          373 a          535 c          570 g          417 t
ORIGIN      
        1 ggaggtcgag gccattctcc acacccatcc ggccgtatcc caggccgtgg tggttggtat
       61 gccgcacccc gtcaacggcg aggtgccgaa ggcattcatc cagcttcgcg agggaatgca
      121 ggttacgtcg cgcgagatca tcgacttctg taaaaaacac ctggcgcact tcaaagtgcc
      181 ccgcagcgtc gagttcatgg agtcgttccc gctctccagt acggggaagg tgctccggag
      241 aatgttgaaa cagccgaagt aaaaaaccgg ggcttcctca attttgggaa gccccggttt
      301 actagccgaa aaaacctcag aatgcccgga agccgacgac gtcctcgcgg atcttcatgt
      361 accgtttctt cgtgatgtcc atgtgctcct tttccatgtc cttgattttg cggaacatgg
      421 cggaagtttc gcctgtggac tccttttcga ggcgctcgta aaactccatc gcgcgcatct
      481 cggagagata tgcggcggag gcgacgcgga gcagcgatgt cgtgtcgccg aagtccttcg
      541 tctgaacgtg cagctccggt tcaacgacca tgttcgggtc gatggggcac tgcgctccga
      601 agagcttttc gtagtagttc atcagacttg cctcgtgttc gctctcccaa tcggagaggg
      661 ccgacagctc tttcttcaag tgctcttcct ggacgctctc cgcccacgtg tggtaaaagt
      721 tcttcgcctc gatttcagcg tggatcccgt agcggagcgc atccttcatg ttaaaactct
      781 tttctgtcat cgtcatccct cctgtgcaag attagattct ttcctgtatt ataccccatt
      841 cccgcaaaaa ttccaaaaac gcaggagctt ttcctcgcct tgacaaaccc ttcccgtcgg
      901 actactatca gaacgaaggc gcgtgcgcct tctttcggaa gatgttcggg aagtccggtg
      961 aaaatccggc gcggacccgc cactgtaagc ctgacgaagc caccgagcca ctggagcgaa
     1021 ccggcgcagc actgcgtacg aagggttccg ggaaggggtg ggggaggatg aaggcgagcc
     1081 aggagaccgg tctttcgaaa ctgcgggaag actcgcgagg aatcgaggcc gccgaagtgt
     1141 ttcccacgca ttccccggag actcgacggg gagcggcgtt ggaagaagat cactaggagg
     1201 ggccgtttcc tgtgtggaac ggtccttttt attatccccg ccaactgcac ggcggtgtgc
     1261 gctcgtttcc tccgggagcc catggcgtcc ggggagcatt ctcccggcta tcactccacg
     1321 ggaggggtaa aggatgagaa aaatgagttg tgcggtcttt gtgtttgttg tgtgtctgtt
     1381 cggcctgctt tcggcaagcg cgttcgcttc ggagcacgcg aagcccgagg ggaaacaggg
     1441 gatactgctt gtcgccttcg ggacgtcgga gcccgacgcc cgctcggcta tcgacggcat
     1501 cgtcgatgcg gtgcggaagg ccttcccgga cgccgaggtg cggctttcgt atacttcgaa
     1561 tattatccgc cggaagatac tcaaggagga ggggctcgtc gtcgacacgc ctccgatagc
     1621 gttggcgaag atgcaggacg aaggctttac gtcggtcgtc gtgcagtcgc tgcacatcat
     1681 ctcgggcgag gagtttcatc agcttgccgg gatagtgcgc gccttctcgt ccatacgcgg
     1741 gaagtacgga ttcgaacgtc tttccctcgg cagaccgctg ctcgacagca tggaggatta
     1801 cagaaagacg gtggacatgc tgaaggcgaa gtacgcctcc tttgcggccg acggcggcgt
     1861 agtgaccttc atggggcacg gtacgcacca cgcgg
//
LOCUS       DAOPKU010000585         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_71697_length_1894_cov_4.696574, whole genome shotgun sequence.
ACCESSION   DAOPKU010000585 DAOPKU010000000
VERSION     DAOPKU010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71697_length_1894_cov_4.696574"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PK849_13870"
     CDS             <1..286
                     /locus_tag="PK849_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67264.1"
                     /translation="HGLVVLLGCSHPGMKNMLDAARNRLKRPIYAVLGGTHLVESKGE
                     GLERSLAYLNDGDIKIVGVSHCTGKEAVERLRESNAGFFHNVTGSSFIVE"
     gene            373..1425
                     /gene="dctP"
                     /locus_tag="PK849_13875"
     CDS             373..1425
                     /gene="dctP"
                     /locus_tag="PK849_13875"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE67265.1"
                     /translation="MCALRKSCWVLLLAVFVLGAFAPVSVEGAEAPKKIVWRSSGHGP
                     ASDPSQIYHDMLCKAITEASGGRLEIKPFVGGSIVPAYKEVDAVHENVLQLCYTCPMY
                     NLDKWSAAGLISSRPGALAGEALRTWFNYGGGADLMNKMMEGYNVMTFPGALSPLPEE
                     VFFHSKKKIESLEDLKAIKARCMGDGGEILKRMGMATVIIPGGELYEAMKRGTIDAFE
                     YSTLASNWNMHFNEVADYVYLSPVRAPSDPQVFFVNKDAWNALPKDLQLLVQTFVDRF
                     SQAQHEFLVYESIIALEKFKEAGNQVLKVPEEVNVALAAEAAKFYEEKIASEPPIFGE
                     IYNSMKKFGEAYQTMK"
     gene            1460..>1894
                     /locus_tag="PK849_13880"
     CDS             1460..>1894
                     /locus_tag="PK849_13880"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HPE67266.1"
                     /translation="MTSLKRFLKKIDALSELSGSVGKWCALLLVFAGTYEAVSRHFFN
                     APTVWAYDTLCMAGGALYLLGASYDYLHEAHTRVDIFYNMLPERGKAFMNVVCALFLF
                     FPLMGVMLWLSVTWAVRAWTINEVFFNSFWYPPAGPYRTIFAV"
BASE COUNT          403 a          538 c          532 g          421 t
ORIGIN      
        1 gcatgggctt gtcgtactgc tgggctgttc ccacccggga atgaagaata tgctcgacgc
       61 tgcacggaac cggctgaagc ggccaattta cgcggtgctc ggcggaacgc atcttgtgga
      121 gtcgaaaggg gaggggcttg aacgttccct cgcctatctg aacgacggag acatcaaaat
      181 cgtaggagta agccactgca caggaaagga agccgtcgaa cgcctccggg agtcgaacgc
      241 cggtttcttc cacaatgtaa ccggcagctc gttcatcgtt gagtaagtct ttctggcttt
      301 ccgaagccga cgcgttcgaa aagcgcggcg gtttctgtaa gacatcatat atctgaagga
      361 ggttgtaaaa agatgtgtgc tctgagaaaa agttgttggg tattgctgct cgcggtattt
      421 gtccttggcg cttttgcgcc cgtttccgtc gagggcgccg aggcgccgaa aaagatcgtg
      481 tggcgttcct ccggccacgg tcctgcctcc gatccgtccc agatttatca tgatatgctc
      541 tgcaaggcca tcaccgaggc ttcgggcggg cgcctggaga tcaagccgtt cgtcggcggc
      601 tccatcgttc ctgcctacaa agaggtcgac gcagtccacg agaacgtgct tcagctctgc
      661 tacacctgcc cgatgtacaa tctcgataag tggtccgcgg cgggcctgat cagctcccgt
      721 cccggcgcac tggccggaga ggctctccgg acgtggttca actacggcgg cggcgctgat
      781 ctgatgaaca aaatgatgga agggtacaac gtcatgacct tccccggcgc gctctctcct
      841 cttcctgaag aggttttctt ccactccaag aagaaaatag agagtcttga ggatctgaag
      901 gcgatcaagg cccgttgcat gggcgacggc ggcgagatcc tgaagagaat gggcatggcg
      961 acggtcatca tccccggcgg cgagctgtac gaagcaatga agcgcggtac catcgacgct
     1021 ttcgagtact ccacgcttgc ctccaactgg aacatgcact tcaacgaagt tgccgactac
     1081 gtctacctct ccccggtacg tgctcccagc gatccccagg tcttcttcgt gaacaaggat
     1141 gcctggaacg cgcttccgaa ggatctgcag ctcctcgtcc aaacgtttgt cgatcgcttc
     1201 tctcaggcgc agcacgaatt cctcgtctac gagtccatca tcgcccttga aaagttcaag
     1261 gaagccggca accaggtgct gaaggttcct gaagaagtaa atgtggcgct tgcagccgag
     1321 gccgcgaagt tctacgaaga gaagatagcc agcgaacctc cgattttcgg cgagatctac
     1381 aactccatga agaagttcgg cgaagcctac cagacaatga aataacgcgt cttcgacgac
     1441 gcgcacaagg agaagcggta tgacctcttt gaaacggttt ttgaaaaaaa tcgatgcact
     1501 tagcgaactg tccggatccg tggggaaatg gtgcgccctt ctccttgtgt tcgccgggac
     1561 gtatgaagct gtctcaagac acttcttcaa cgcccccacg gtctgggcgt acgatacgct
     1621 ctgtatggca ggaggcgccc tctatcttct gggcgcttcc tatgactacc tccacgaggc
     1681 gcacacgcgt gtggacattt tttacaacat gctccccgaa cgggggaagg cgttcatgaa
     1741 cgtcgtctgt gcgctcttcc ttttcttccc tctgatgggg gtcatgctct ggctctccgt
     1801 tacgtgggca gtgcgcgcct ggacgatcaa cgaagtcttc ttcaacagct tctggtaccc
     1861 cccggcgggg ccgtaccgta ccattttcgc agtc
//
LOCUS       DAOPKU010000586         1892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_71795_length_1892_cov_5.371802, whole genome shotgun sequence.
ACCESSION   DAOPKU010000586 DAOPKU010000000
VERSION     DAOPKU010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71795_length_1892_cov_5.371802"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PK849_13885"
     CDS             complement(<1..941)
                     /locus_tag="PK849_13885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE67267.1"
                     /translation="MNTINLRLMLIHRGTRTHSITQIEPIGLMSLAEMMTGQGIDTAV
                     FSGELLKGLEFLNTTGCGRNTVVGLYCDYENQSAVESFSRVIKERFGAVVLVGGPQTV
                     GLGEAFLRASRADGIVRGEGEYSLLAAIRTLREERREQWETIPGMCSFTKGGAYYDNG
                     IYPVIENLDSIPGISGTIKSAQHRGINHMAVMTGRGCPFHCSFCYEGANSKNVRQRSV
                     ANVMHEIRRRLQENPNVKYLFFCDDTFTLDTERVRAFCEELKELRKEHDFVWFADAHV
                     NVVINHPEIVKMMVDAGLVRMQIGIESCNQHIIDIYN"
     gene            complement(997..>1892)
                     /locus_tag="PK849_13890"
     CDS             complement(997..>1892)
                     /locus_tag="PK849_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67268.1"
                     /translation="GHPGFLARRPELIAEMIESGMVRMQIGMESGDGRVLQAYGKQAV
                     PEDVKNVVDTCCREGLPQLAGNFIIGGAFETRRTLETTTGFALDLLHRAPGMLDLSTT
                     FLMPLPGTRISTHPGSFGIALEDRDCLTSIEDFPVNSTLELSVPEICRERSVFLTHIS
                     NAMKAQYANGRIPPERIRRDFELAFRYGIAAGYLKFIYGKDPLLVAYYRKLTDYKGLL
                     KEWNELEGAEKTTSFIQRVPDFSLVDFTVLSGAETDILLDAGSSSVKETAKKLNLSIE
                     ATSVCLERLSAQHLTLFSPCL"
BASE COUNT          383 a          558 c          516 g          435 t
ORIGIN      
        1 ttgttataga tgtcgatgat gtgctggttg cagctttcaa tgccgatctg catcctgacc
       61 agtccggcgt ccaccatcat ctttacgatt tcggggtggt tgatgacgac attgacgtgg
      121 gcgtcggcga accagacgaa atcgtgttct ttccgcagct ctttgagttc ctcgcaaaag
      181 gcgcgcaccc gttctgtatc cagggtaaag gtgtcgtcgc aaaaaaagag gtactttacg
      241 ttgggattct cctgcagccg gcgccggatc tcatgcatga cgttcgccac gctgcgctgc
      301 cggacgttct tgctgttggc cccttcgtaa cagaaagagc agtgaaaagg acaccctcgc
      361 cccgtcatca cggccatgtg attgatgccg cggtgctggg ccgatttaat agttcccgaa
      421 ataccgggga tggagtccag attttcgatg acaggataaa ttccgttgtc gtagtatgct
      481 ccgccctttg tgaacgaaca catcccgggg atggtctccc actgttccct ccgctcttct
      541 ctcagtgtgc ggattgctgc gagcagagag tactctccct cgccccgtac gattccgtcg
      601 gcgcggctcg ctcgtaaaaa cgcctctccc aacccgacgg tctgggggcc gccgacgaga
      661 acgacggcgc cgaaacgctc cttgatcacc ctggaaaagc tctcaaccgc cgactgattc
      721 tcgtaatcac agtataatcc caccactgta ttccggccgc accctgttgt gttcaggaat
      781 tccaggcctt tcaacagctc tcctgaaaaa acggccgtgt cgattccctg gccggtcatc
      841 atctccgcca gcgacataag gccgatgggc tctatctggg tgatcgaatg ggttctcgtt
      901 ccgcgatgaa tcagcatcag tctcaggttg atagtgttca ctccgggcct ccatagagta
      961 gtgtgtacga caggttcgct ctcatcgcat tccgtttcac aggcagggcg agaagagcgt
     1021 cagatgctgc gcgctcagtc tttcgaggca gacgctcgtt gcttcgatgg acagattcag
     1081 ttttttcgcc gtctccttca ccgaagagga accggcgtcc agcaggatat ccgtttccgc
     1141 gccgctcaag actgtaaaat ccaccagcga aaaatcagga acgcgctgga tgaaggaggt
     1201 tgtcttttcc gctccttcaa gttcgttcca ttctttcaga agtcccttat agtcggtaag
     1261 tttccgatag taggcgacca gaagcgggtc cttgccgtag atgaacttca ggtaccccgc
     1321 cgcgatgccg taccggaacg ccagctcgaa gtcgcgcctg atccgctccg ggggaatccg
     1381 gccgttcgcg tactgcgcct tcatcgcgtt ggaaatatgc gtgagaaaga ccgaccgttc
     1441 acggcatatt tccggaacgg aaagctcgag cgtactgttc accggaaagt cctcgatgga
     1501 cgtcagacag tcccggtctt caagggcgat gccgaagctc ccgggatgtg tggaaattcg
     1561 cgttcccggc agcggcatca gaaaagtggt ggagagatcc agcattcccg gcgctcgatg
     1621 cagcagatca agagcgaagc cggtggtcgt ttcgagggtg cgccgggttt caaaagcccc
     1681 cccgatgatg aaattcccgg cgagctgggg cagcccctcg cggcagcagg tatccaccac
     1741 gttcttcacg tcctccggaa cggcttgctt cccgtatgcc tgaaggacgc gaccatctcc
     1801 ggattccatg ccgatctgca tcctgaccat gccgctttcg atcatttcgg ctatcagctc
     1861 cgggcggcgc gctaaaaaac ccgggtgccc tt
//
LOCUS       DAOPKU010000587         1885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_72182_length_1885_cov_4.676503, whole genome shotgun sequence.
ACCESSION   DAOPKU010000587 DAOPKU010000000
VERSION     DAOPKU010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72182_length_1885_cov_4.676503"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1333)
                     /locus_tag="PK849_13895"
     CDS             complement(116..1333)
                     /locus_tag="PK849_13895"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HPE67269.1"
                     /translation="MKQAVLIALGDELLSGARREGNCGWLANRLALAGWKVLRMEIIP
                     DGLGALEDTLALWAGKTDLIVISGGLGPTHDDCTRKALARFLNAPLQVNAEAYNRILA
                     RYPSEMRENLELCRISQGSVPEGAQAIHNPRGSALGISFLMEGTKICAFPGVPDEFKA
                     MAEQELACEMSVSDCRMRSLIVVGWPESLLKDRIAPVVDDPSLHISILPSSGTVEIIL
                     RGEPVRIECAARTLRELLPGDCLPEGCFSVAEDLLREARKRNVTLAFAESCTGGLLGA
                     ALTDISGSSDVFLGSAVCYSNSAKTHILSVSDELLESFGAVSSECAIAMAKGALNQFG
                     SELALSVTGVAGPGGGSEEKPVGAVWFALAGLCGERAFLHHFPGDRLQIRRSAMNTGL
                     LLLRKTVMELNIL"
     gene            complement(1371..1862)
                     /locus_tag="PK849_13900"
     CDS             complement(1371..1862)
                     /locus_tag="PK849_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPE67270.1"
                     /translation="MLKNMISSRKYQGTKDIAWCISTLCGLGFFSSMPGTLGSVAAFL
                     FFVVIPVPWYLILATAILGVSVSQLYSKRIGVEDPKEVIIDEVVGVWIAMYGMPSGFF
                     LPALFLFRVVDILKPFPVNASEKLPGGWGIMADDIVGGILVNLLLTFIRWLYIGGGWS
                     HIL"
BASE COUNT          506 a          521 c          439 g          419 t
ORIGIN      
        1 cccgataccc atttcaaatc cggcaaaact aaacgaaggg tgtccacagt ttgccaaagt
       61 ttcttttgta ctgcaggagg caactcgatg caaaagaaag aacgcagtat tccattcata
      121 gaatgttcaa ctccattacc gttttccgga gcagcaaaag ccctgtattc atcgcacttc
      181 tacgtatctg gagtctgtcc ccggggaaat gatgcaaaaa tgcacgttcg ccgcaaagac
      241 ctgcaagagc gaaccagacg gcgcctaccg gtttttcttc agagccgcct cctggtcccg
      301 cgactcctgt gacagagagc gccaactcgg agccaaactg attcaaagca cccttcgcca
      361 tggcgatcgc gcactcggaa cttacagccc caaaactctc aagcagctcg tctgaaactg
      421 agagtatatg tgtttttgcg ctgttactgt aacagactgc gcttcctaaa aacacatccg
      481 aactccctga gatgtctgtg agcgcagccc caagaagacc tccagtgcat gattccgcaa
      541 aggcaagtgt gacgtttctt ttccgggcct ctcgaaggag atcctctgct acggaaaaac
      601 agccttccgg caggcagtcg ccagggagga gctctcgtaa cgtgcgagcc gcgcattcta
      661 tgcgaacagg ttctcctctg agaataattt ccactgttcc ggaagagggg agaatggaaa
      721 tatggaggga gggatcgtca accacaggag caattcggtc tttcaaaaga ctctccggcc
      781 atccgacaac aatgagtgaa cgcatcctgc agtcacttac agacatctcg caggcaagtt
      841 cctgctcagc cattgctttg aactcatcgg gaacacccgg aaaggcgcag atctttgtcc
      901 cttccatcag aaaggatata ccaagagcgg agccgcgcgg gttatgtata gcctgggcgc
      961 cttcaggaac tgagccctga gaaattctgc ataattcaag gttctctctc atctcggaag
     1021 gatatcgtgc taaaattctg ttatatgctt ccgcgttcac ctgaagcggg gcgttcaaaa
     1081 accgtgcaag ggccttcctt gtgcaatcat catgtgttgg cccgagccct cctgagatga
     1141 caataaggtc cgtttttcca gcccaaagag ccagagtgtc ctccagcgct ccgagtccgt
     1201 cgggtatgat ttccatccgg agaactttcc accctgcaag agcaagacga ttcgcgagcc
     1261 atccgcaatt cccctcgcga cgtgcaccgc tgagaagttc atcaccaagg gcaatgagga
     1321 ctgcctgttt catggtatgc ctcctttttc cgaaaagccg tcgttccttt ttacaggata
     1381 tgactccagc cgcctccgat gtagagccag cggataaagg tcaggagtag attgacgaga
     1441 atgcctccga cgatatcgtc cgccattatt ccccatcctc cgggaagttt ttccgaggcg
     1501 ttcacaggaa acggcttcaa tatatccaca acccggaaaa gaaagagcgc tgggagaaaa
     1561 aaaccggagg gcattccata cattgctatc cagacgccga caacttcgtc aataattact
     1621 tctttcggat cttcgacgcc gattcttttg gaatatagct gcgacacaga gactcccaaa
     1681 atggctgtag caagaatcag gtaccacgga acaggaatga caacaaagaa aagaaacgcc
     1741 gctaccgacc cgagggttcc cggcatcgaa gagaaaaaac ccaatccgca aagagtgctg
     1801 atacaccatg cgatatcttt agttccctga tatttccgcg aagagatcat gttcttcagc
     1861 atctgttatc aacacctcca caaaa
//
LOCUS       DAOPKU010000588         1882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_72375_length_1882_cov_3.787630, whole genome shotgun sequence.
ACCESSION   DAOPKU010000588 DAOPKU010000000
VERSION     DAOPKU010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72375_length_1882_cov_3.787630"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..727)
                     /locus_tag="PK849_13905"
     CDS             complement(26..727)
                     /locus_tag="PK849_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPE67271.1"
                     /translation="MDENSKRTFPLVEAMKLTNQFDTARQEHRDRLGGTLVKRLEERG
                     FHALYVQTKEEALKEVVKLIPEGASVGVPGSVTIREIGAMEDLAKRGCTVIHHWNPSL
                     SAEERMDTLQNELLADYFLTSSNAITLDGLLVNIDGNGNRVSGMAWGKNTLIFVIGLN
                     KVTNSLDEAIARTRNQATPPNALRLKLQTPCASTGRCSDCNSPDRACKALLVLERATG
                     GRTSHVILVGEDLGF"
     gene            845..1189
                     /locus_tag="PK849_13910"
     CDS             845..1189
                     /locus_tag="PK849_13910"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPE67272.1"
                     /translation="MRTTKEETVLAKRVYLHDLYDLYAPLLTEKQREAWDLHEFADFS
                     LAETAEKLGISRQGVSDLITRSRDRLEELESLLGFFHKEDAFEEEIRELRRRIEHLSS
                     GRGGAMQKAEGN"
     gene            1192..>1882
                     /locus_tag="PK849_13915"
     CDS             1192..>1882
                     /locus_tag="PK849_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPE67273.1"
                     /translation="MFDALRERFEAVFDKLKGRGKLTEADVETALRDVRRGLLEADVD
                     YKVVKSLLEAIKTRAIGRDVLESITPGQHVAAIVYEELAELMGKEPVPLVISPKPPTV
                     YLMVGLQGGGKTTSTVKIARKLSGAHKPLVVACDLRRPAAVEQLRVLAAQSIISFFGP
                     ENGEKNVLNVVKAALSYADDHLNDVVLLDTAGRLHADEDLMDELVAMKKSAPPTEILL
                     VVDAMTGQEAVK"
BASE COUNT          430 a          473 c          512 g          467 t
ORIGIN      
        1 caccctttat ggcatttgtt cggttttaaa agcccaggtc ctctccgaca aggatgacat
       61 gcgacgttct cccgccggtt gcgcgctcaa gaacgaggag cgctttacag gccctgtcgg
      121 gagaattgca gtccgagcaa cgtcctgtgc tggcgcacgg cgtctgcaat tttagccgaa
      181 gagcattcgg gggagtagcc tggttgcgcg ttcgtgcaat cgcctcgtcc aaactgttcg
      241 tcactttgtt cagtccaata acgaagataa gcgtgttttt cccccacgcc atgccgctta
      301 ccctgtttcc gtttccgtcg atattgacca gcaatccgtc gagagtgatg gcattcgagc
      361 ttgtaaggaa gtaatcggcc aagagctcgt tttgaagcgt gtccatgcgt tcttccgcag
      421 acagggacgg gttccagtga tggattacgg tgcatcccct cttcgcaagg tcttccatgg
      481 cgccgatttc acgaattgtg acgctgccgg ggacgccgac ggaggcgccc tccgggatga
      541 gctttacaac ctctttgagg gcctcttctt ttgtctgaac atagagagcg tgaaagcctc
      601 tttcttccag cctttttacc aatgtaccgc ccagccggtc gcggtgttcc tgtcgggccg
      661 tatcgaactg attcgtcaat ttcatcgcct ccacaagcgg gaatgtccgc tttgaatttt
      721 cgtccatcat atcatttttc tctccgaacg tttcgctccg gaatatagaa tgctgtcaag
      781 agaaaaagct tgacactttt tgcgaagggg gatataattc cacagttcca ggggaggctg
      841 cgagatgcgc acgacgaaag aagaaacagt tttagccaag agagtgtatc tccacgactt
      901 gtacgacctt tatgctccat tgttaactga aaagcaacgt gaggcttggg atctccatga
      961 gtttgcggat ttctctcttg ccgagacagc cgaaaaactt ggaatcagcc gtcagggcgt
     1021 gtccgatctt attacgcgaa gtcgtgatcg acttgaggag ctggaaagcc ttcttggttt
     1081 ttttcacaaa gaagacgcgt ttgaagaaga aatccgcgag cttcggcgcc gtattgagca
     1141 tctttcttcg ggccggggcg gggcgatgca gaaagcggag ggaaactgaa aatgttcgat
     1201 gcgcttcgtg aacgttttga ggcagtgttc gacaaactca aagggcgcgg caaactcacc
     1261 gaggcagatg tggaaactgc gttgagggat gtgcggcgag ggcttttaga agccgatgtc
     1321 gattataaag tggtaaagtc tcttctggag gccataaaaa ctcgcgccat aggaagagat
     1381 gttctcgaat ccatcacccc ggggcaacat gtcgctgcaa tcgtctatga agaacttgca
     1441 gagttgatgg gaaaagaacc tgtacccctt gttatttctc caaagccgcc gactgtctac
     1501 ctcatggtcg gtttgcaggg cggaggaaaa acaacgagca cggtgaagat agcgcgaaag
     1561 ctttccgggg cgcataaacc cctcgtggtt gcttgcgacc ttcgtcgccc cgccgctgtc
     1621 gagcagcttc gggtactggc tgcacaatcg ataatttcat tttttggtcc tgaaaatgga
     1681 gaaaaaaatg tcctcaacgt cgtaaaagct gctctgagct atgcggatga ccatctgaac
     1741 gacgttgtgc ttcttgatac ggccggacgt ctccatgccg acgaggattt aatggatgag
     1801 cttgtcgcca tgaagaagtc cgctcctcct accgagatac tccttgtcgt cgacgccatg
     1861 accggccagg aggcggtaaa gg
//
LOCUS       DAOPKU010000589         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_72451_length_1881_cov_3.355969, whole genome shotgun sequence.
ACCESSION   DAOPKU010000589 DAOPKU010000000
VERSION     DAOPKU010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72451_length_1881_cov_3.355969"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK849_13920"
     CDS             <1..230
                     /locus_tag="PK849_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HPE67274.1"
                     /translation="VVFSAFPVLVYQGALTLLAEGVQRVMSPEVIAEVSAVGGLMLLG
                     IGLAILEIKRIKVINMLPALILAGILASFFR"
     gene            complement(319..1068)
                     /locus_tag="PK849_13925"
     CDS             complement(319..1068)
                     /locus_tag="PK849_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPE67275.1"
                     /translation="MNHPFMDIPITAAPSFSWEASRAVPIDECGEEMVAASLIPEKIL
                     VRPQYYLHMLEGAIPECYVRRSVLERLIEAADMLPDGRRLVLLDTWRSRNIQTTLFQK
                     FRSELREKMPLLDDSEITVLASQFVAPPSLHPERPSPHITGGAVDLTIVDKMGFCLPM
                     GTGFDETSEKSATVWYEKKFDSKEAMSEDETEALHNRRLLYYVMHKAGFTNYPDEWWH
                     YDYGNQNWALLRGKKHAIYGVTEPAFRWRSL"
     gene            complement(1093..>1881)
                     /locus_tag="PK849_13930"
     CDS             complement(1093..>1881)
                     /locus_tag="PK849_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE67276.1"
                     /translation="GGLFAAGILPGILLGGAFMGMNYVYSHRYQVPVADFEEVRKMKK
                     EILKRSLVALFAPVIIIGGIITGVVTPTESGALAVAYCLVAGTLYTRQMTFRVLAESL
                     YETVRLTSSIFLIMGAAMVIGWLLKWEQVPQKFASIILQAGLLDKPWLLMVVLSAIIF
                     PVGMFMEEVSTLALLTPIFAPIALKAGIDPFHFGVVMTLNVTIALITPPMGACNYIVA
                     AVGRVKLSDVFFHIWPFIGVALLVLLAIISFPPLTTLIPRMLNL"
BASE COUNT          425 a          516 c          527 g          413 t
ORIGIN      
        1 gggttgtgtt ttccgccttc cctgtccttg tgtaccaggg agccctgaca ttgcttgcgg
       61 agggcgtaca gcgtgtgatg agcccggagg tgatcgcgga ggtgagcgcg gtaggcgggc
      121 ttatgctcct gggcatagga ctcgcgatac tggagatcaa gcgcatcaag gtgatcaaca
      181 tgcttcccgc ccttatattg gcggggatcc tggcgtcatt ctttcgctga tccgcttttc
      241 gctgacctgc taaaaagaaa cggaaggccg gagtatggat tctccggcct tccgtttctt
      301 ttttagctct tcttcgtatc agagggaacg ccagcggaac gcgggttctg ttacgccgta
      361 gatcgcatgt ttcttcccgc ggagcagcgc ccagttctga ttgccgtagt cgtagtgcca
      421 ccactcgtcg gggtagttgg tgaatcccgc cttgtgcatc acgtaataca gcagccgccg
      481 gttgtgcagg gcctccgtct catcctcgga catggcctct tttgaatcga attttttctc
      541 gtaccacaca gtggccgact tctcggaagt ctcgtcgaac ccggttccca tcggcagaca
      601 gaagcccatt ttatcgacga tggtcaggtc taccgctccc cccgtgatat gaggcgaggg
      661 gcgctcgggg tgcagcgacg gcggcgcaac aaactgagag gcgagaacag taatttccga
      721 atcatcaaga agcggcattt tctcacggag ttcggagcgg aatttctgaa aaagggtcgt
      781 ctggatattg cgggaacgcc acgtatccaa gagcacaagc cggcgcccgt cgggcagcat
      841 atctgctgct tcaataaggc gttcgagcac cgaacggcgt acgtagcact ccgggatggc
      901 gccttcgagc atatggagat agtactgggg ccgaacaagg atcttctccg gtataaggct
      961 tgcggccacc atctcctcgc cgcactcatc tatcggcacc gctctcgaag cttcccacga
     1021 aaagcttgga gcggctgtaa tcggtatgtc catgaaggga tgattcattt ttcttctcct
     1081 tccatttccg tgttacaggt tcagcatccg cggaataagc gtagtcagcg gaggaaatga
     1141 tatgatcgcg agcagaacaa gcagggcgac gccgatgaaa ggccagatgt ggaaaaaaac
     1201 gtccgaaagc ttcaccctcc ccacggctgc gacgatatag ttgcatgctc ccattggggg
     1261 agtgatcagc gcaatcgtta cattgagcgt catcacaact ccgaagtgaa aggggtctat
     1321 gcccgctttc agcgcgatag gcgcaaaaat aggggtaaga agcgccaaag ttgaaacctc
     1381 ttccatgaac atgcccaccg gaaagatgat ggcgctgagc acgaccataa ggagccacgg
     1441 tttgtccaga agccccgcct ggaggatgat agaagcaaac ttctgcggta cttgctccca
     1501 ctttaaaagc catccgataa ccatggccgc tcccatgatg agaaagatcg agctggtcag
     1561 tcggacggtc tcgtagagcg actccgcgag tacccggaac gtcatctgcc gcgtgtacag
     1621 cgttcctgcg accaggcaat acgcaaccgc gagcgcgccg gattccgtcg gggtgacgac
     1681 ccctgtaata attcctccta tgataatcac gggcgcgaaa agtgcgacca gcgaacgttt
     1741 caggatctct tttttcattt tacgaacttc ttcaaaatcg gcgacgggaa cctggtaccg
     1801 gtgagagtag acgtagttca ttcccataaa agctcctccc agcaggatac cgggcaggat
     1861 accggcggca aacagcccgc c
//
LOCUS       DAOPKU010000590         1872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_72915_length_1872_cov_4.860759, whole genome shotgun sequence.
ACCESSION   DAOPKU010000590 DAOPKU010000000
VERSION     DAOPKU010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72915_length_1872_cov_4.860759"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..1831)
                     /locus_tag="PK849_13935"
     CDS             complement(197..1831)
                     /locus_tag="PK849_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPE67277.1"
                     /translation="MVRFDELPIPFAAVATDLETGEKVVLRSGSLASAMRASMAIPAL
                     FDPWPMGDRLLVDGGLVSNLPVETAKELFPGYPVIAVDVTGKLKTKKQIRSLVDVIDQ
                     SITIFTHRNVQHESPMADLLISPPVEGVSIFDLSNAEVVIEGGKQATLAMLQAIRKLA
                     ESAPPVAQRETLHLDTRVASVKVEGATLELASSIVKKYGSWVGKPLSDIEVLRASREI
                     SERDDILSVDYRFEEAGPNVDVVFAVQRKPALEINFGGYTTNLHPHRWLFVSGIRRDL
                     FKDADALKFNIKVGEQWGVDASYFDSPGKTPWEIRLSAYNWELETANAGKRSWDRYAL
                     GVLKHFNGRSFNGGFGYAFERIHTGGDEFDASGPTLFLASDTLNDPVDPTDGALLYMQ
                     AWWPDFDEPLFRLSYFRASRVANDWRFYFRAGFAEGDMKREGRAVYLGASEELYSYSG
                     NPIEAERMAWWNIAFRRILMKSWWGALNTEFFGGMGFAFDDEWDRFRDVWEAGVSFSI
                     PGVLFDGKLMFIYNDESDLKIGFFLGSPIWGKYPLP"
BASE COUNT          389 a          557 c          519 g          407 t
ORIGIN      
        1 cgtatgacca ttcagcccct gacaggcgcg taatccgcca gctctgcgac cttgatgatg
       61 tcgagatacc actgggaata gtcttccgtt ctcgatgtta tgttccgtgc catttctctt
      121 cctcctgttt tccgtatccc ccgtttcggg gagtatcaag cgctgtacag cgcgttcaca
      181 gttatgcccg gaaatatcag ggcagcgggt atttccccca gatgggactg ccgaggaaga
      241 accctatttt caagtcgctt tcgtcattgt agatgaacat gagttttccg tcgaagagca
      301 ctcccggtat tgagaaggaa actcccgcct cccacacgtc gcggaaccgg tcccattcgt
      361 cgtcgaaggc aaatcccatg ccgccgaaga actccgtgtt cagggctccc caccagctct
      421 tcataaggat acgccggaag gcgatgttcc accatgccat ccgctccgct tcgatcggat
      481 tgcctgaata gctatagagt tcttcggagg ctccgagata gaccgcccgt ccctctctct
      541 tcatgtcgcc ctcggcaaat cccgctctga agtagaatcg ccagtcgttg gccacgcgcg
      601 aggccctgaa gtacgagagc cggaagagcg gttcgtcgaa gtccggccac cacgcctgca
      661 tatagagaag cgccccgtcg gtcgggtcga ccggatcgtt gagcgtatcg gaagccagga
      721 agagagtggg acctgacgcg tcaaactcgt cacccccggt atggattcgt tcgaatgcgt
      781 atccgaaacc tccgttgaaa gagcggccgt taaagtgttt gagaacgccg agagcatacc
      841 ggtcccatga gcgtttgcct gcattcgccg tttcaagctc ccagttgtag gcggagagcc
      901 gaatttccca cggcgtcttc cccggcgagt cgaagtagct cgcgtcgacg ccccactgtt
      961 cgccaacctt gatattgaac ttcagagcgt ccgcgtcctt gaagagatcc cggcgaatac
     1021 cgctgacgaa gagccagcga tgggggtgga ggtttgtcgt gtaccctccg aaattgatct
     1081 caagcgcggg ttttctctgt acagcgaaga cgacgtccac attcggtccg gcctcctcga
     1141 aacggtagtc cacggagagg atatcgtcgc gttcgcttat ctccctgctc gcccgcaaaa
     1201 cctcgatatc gctcagcggt ttacccaccc atgagccgta ctttttcacg atggaggatg
     1261 cgagctcgag cgtagctccc tctaccttga cagaggctac gcgcgtgtcg aggtgaagcg
     1321 tttcccgctg cgccacagga ggagcggact cggcaagttt tctgattgcc tggagcatgg
     1381 cgagcgtcgc ctgcttgccg ccttcgatga ccacctccgc gttcgaaagg tcgaaaatgc
     1441 tcacgccctc taccggggga gaaatgagga gatccgccat cggactctca tgctggacgt
     1501 tcctgtgcgt aaagatcgta attgactggt cgatcacatc cacaagcgag cgaatctgtt
     1561 ttttcgtctt tagttttcct gtcacgtcca cggcaatcac cgggtacccc ggaaaaagct
     1621 cctttgcggt ctcgacggga agattcgaga ccagaccgcc gtcgaccagc agtctgtcgc
     1681 ccatgggcca cgggtcgaag agcgcgggaa tagccataga ggcgcgcatc gccgatgcaa
     1741 ggcttcctga gcgaaggacg accttctctc cggtttccag atcggtggcg actgcggcga
     1801 atggaatcgg cagctcgtcg aaacgaacca cctagatacg ggaagcaagc tgtgcgaagc
     1861 gttcgaggag ct
//
LOCUS       DAOPKU010000591         1871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_72982_length_1871_cov_3.751652, whole genome shotgun sequence.
ACCESSION   DAOPKU010000591 DAOPKU010000000
VERSION     DAOPKU010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72982_length_1871_cov_3.751652"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PK849_13940"
     CDS             complement(<1..1143)
                     /locus_tag="PK849_13940"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPE67278.1"
                     /translation="MAKYVFVTGGVVSSLGKGITAASLGVLLKKRGLRVSIIKLDPYL
                     NVDAGTMNPFQHGEVFVTDDGAETDLDLGHYERFIDESLSANNNVTTGKIYSSVIAKE
                     RRGDYLGGTVQVIPHITNEIQERILRAGEKSDIVIAEIGGTVGDIEGQPFLEAIRQMS
                     SRVGRENVLYCHVTLVPWLEAARELKTKPTQHSVQELRRIGILPQILVCRSHYAIEEE
                     MRDKMALFCNVPPEAVIQALDEPTIYKVPLSLYEQKFDLLVLKGLSLESPREPDLSDW
                     RRVVDGFCSPAGAVDIVMVGKYINHKDAYLSVVEALHHAGIHHDVKVNIRSVEAEEIE
                     EHGSEKILSGAHGILVPGGFGSRGIEGKIAAARFARERKIPYLGLCL"
     gene            complement(1279..1797)
                     /locus_tag="PK849_13945"
     CDS             complement(1279..1797)
                     /locus_tag="PK849_13945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPE67279.1"
                     /translation="MPPSRVRLAARATAPRQSLTRCSTESISTASRAPAATAKVSSSS
                     SRSYRWPVVGKINSPFGWRRHPVTRRRDFHTGIDIKAPRGRTIQAARGGRVEYSGWMG
                     GYGRVVVIDHGDGHSTLYAHCSSLAVRKGNKVSQGQAIATVGTTGRTTGPHLHFEVRR
                     GNNPVNPLGMLR"
BASE COUNT          382 a          584 c          513 g          392 t
ORIGIN      
        1 cagacagaga ccgagatagg gaattttccg ctcacgggca aagcgagccg cagcgatctt
       61 tccctcgatt ccgcgcgaac cgaaaccgcc ggggaccaga ataccatgcg cgcctgaaag
      121 gatcttctcg cttccatgct cttcgatctc ttctgcttcc actgaacgta tattcacctt
      181 cacgtcatgg tgaatcccgg cgtgatggag cgcttccacc acgctcaggt aggcgtcctt
      241 gtgattgata tatttcccca ccatgacaat atcgactgct cccgcggggg agcagaatcc
      301 gtcgacgact cttctccagt cggaaagatc cggttcgcgg ggagattcaa gagaaaggcc
      361 cttgagtacg agcaagtcga acttttgttc atacaggctg agaggcacct tgtagatcgt
      421 cggttcgtca agagcctgaa tgactgcttc cggcgggacg ttgcagaaga gcgccatctt
      481 gtcgcgcatc tcttcctcga tagcgtaatg ggaacggcac accaggattt gagggaggat
      541 accaatacgc cgcagctcct gaacgctatg ctgcgtcggt ttggtcttca attcgcgcgc
      601 cgcctcaagc cagggcacaa gcgtcacgtg acaatagagc acattctcgc gtcctaccct
      661 ggacgacatc tgacggatgg cctccaggaa gggctgtcct tcaatatcgc ccacggttcc
      721 gccgatctcc gcgatgacaa tgtcgctctt ctcgcccgcc cggaggatac gctcctgaat
      781 ctcgttggtg atgtgcggaa tgacctgcac cgtccccccc agataatcgc cgcggcgctc
      841 cttggcgatg acggaagagt agatcttccc cgttgtcaca ttgttgttcg cgctcagcga
      901 ctcatcgata aaccgttcgt aatgccccag atccaggtcg gtttcggcgc cgtcgtcggt
      961 cacgaaaacc tctccgtgct ggaagggatt catcgtcccc gcgtccacgt tcaaatacgg
     1021 atcaagtttg atgatggaga cgcgcagccc cctctttttg agcaacacgc ccagcgacgc
     1081 cgcagtgata ccctttccca acgacgacac cactccgcct gtgacaaaca cgtacttcgc
     1141 catttccctc atcctccact agtattcaga aaaaatcgtt cacgagacga acacgtcaac
     1201 gcatcaaaaa aacatggacc agggagaacc ccggtccaga atatcgaaag agaatccatc
     1261 cgtcatggag gatattgcct atcttaacat ccccagcggg ttcaccggat tgtttcccct
     1321 gcggacttcg aagtgcaggt gcggaccggt tgtccttccg gtggtgccga cggtcgcgat
     1381 agcctgtccc tgagaaacct tgtttccctt acggaccgcg agggagctgc agtgagcgta
     1441 cagggtcgaa tgaccgtctc cgtggtcgat gaccacaact ctgccgtagc cacccatcca
     1501 gccggagtac tccacacgtc ctccgcgcgc cgcctgtatt gttctgcccc ggggggcctt
     1561 gatatcaatc cccgtgtgga agtcgcgccg gcgtgtcacc ggatgacgcc tccacccgaa
     1621 cggactgttg atctttccca ccacaggcca ccgataactc cgcgaggagc tgctggaaac
     1681 cttcgccgtg gccgcagggg cgcgcgacgc ggtgctgatg ctctcggtgc tgcatctggt
     1741 caaggactgg cgcggggccg tggcgcgcgc ggcaagcctg acgcgcgagg gcggcatctt
     1801 tgtgtcctcc acgatgacgg gcggggatgc gggcgcgggc gcggaacacg tggttcttca
     1861 ccgatgcgtc c
//
LOCUS       DAOPKU010000592         1870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_73007_length_1870_cov_13.944353, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000592 DAOPKU010000000
VERSION     DAOPKU010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73007_length_1870_cov_13.944353"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PK849_13950"
     CDS             <1..253
                     /locus_tag="PK849_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67280.1"
                     /translation="TFREEFSSSRRAERLTKNSATVETSGKRTVRRMRNCCEDIACGG
                     KEKRSGRYEKNAINTKNPSGPVAKTGVKSVHHKQTPPER"
     gene            261..1730
                     /locus_tag="PK849_13955"
     CDS             261..1730
                     /locus_tag="PK849_13955"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPE67281.1"
                     /translation="MPRKTAPFNFESAPEQRSLTAKPGGILLAELFHASGCAAAADAI
                     GVRSSRGYSDADHVLSSVLLNALGFDAGDDLEQLRSDGALVSAVHLLNEHKGKERRTV
                     NAFPSPSAFRAFLTDAGSTEERVRGFSGLCSVPVRCAQEMKPQEKITVDMDATIIPTE
                     RGDDVRCNYKGERSHMAFNSYVPELDMVVHSEFCPGNVSPRDDQLGLLRRTLSKLPEG
                     VKEVETRIDSAGYCMDLIKYCCGGESPYGVIRFSISVPMYKTYREEIERLSEKAWSPF
                     VDENGREETDRRWAEVNIVPTSLVSSEPYPEIRFIAVRERMKHPKLKERMDAAQMELA
                     IEEIEGEHPKTERLHLTCMDGTVWKVFLMVTNDFERNGQHALEHHWKRCGKSEEVHGI
                     LKNDLAGGHVPSADFGSCAVWWYLCVLSYNLQRLMTHRLFPEEWARAHQKRLLATIYS
                     TPAKMVRHGKQIVIRVFGLLGRLIEAAHWKVLRLGRLNI"
BASE COUNT          430 a          512 c          597 g          331 t
ORIGIN      
        1 cacctttcgg gaggagtttt cgagttcgag gcgggcagaa cggcttacga aaaattctgc
       61 gaccgtggaa acatcaggaa agcgaactgt tcgccggatg aggaattgct gcgaagatat
      121 cgcttgcggc ggaaaagaaa agaggagcgg aagatacgag aaaaacgcaa taaatacaaa
      181 aaacccctct ggtcccgttg cgaaaacggg tgtaaagtcg gttcaccaca aacaaactcc
      241 accagagagg tgaccgtatt atgccacgaa agaccgctcc gttcaacttc gaatccgccc
      301 ccgaacagag aagccttacc gcgaagccgg gaggaatcct tctggcggaa ctcttccacg
      361 catcgggctg cgcggcggcc gcggacgcaa taggggttcg gagcagcagg ggctactccg
      421 acgccgacca tgtgctttcg tcggtgctgc tgaacgctct cggcttcgac gcgggcgacg
      481 atctggaaca gctccgctcc gacggggcgc tcgtctccgc cgtacatctt ctgaacgagc
      541 acaaggggaa ggagagacgc accgtgaacg cctttccctc cccttcggcc ttccgcgcct
      601 ttctgaccga cgcgggaagc acggaggaga gagtgcgcgg attctccggt ctgtgctccg
      661 tccccgtccg ttgcgcgcag gaaatgaagc cgcaggagaa gatcaccgtc gacatggacg
      721 ccaccatcat ccctacggag cgcggcgacg atgtccggtg caactacaag ggcgagagat
      781 cgcacatggc gttcaacagc tacgttcccg agctggacat ggtggttcac agcgaatttt
      841 gtcccggaaa cgtatcgccc cgcgacgacc agctcggact gcttcgaaga acgctctcga
      901 agctgcccga gggggtgaag gaggtcgaaa cgcgcatcga cagcgccgga tactgcatgg
      961 atctcatcaa gtactgctgc ggcggagaga gcccgtacgg cgtcatacgc ttctccatct
     1021 ccgttcccat gtacaaaacg taccgggagg agatcgaaag actttccgag aaggcctgga
     1081 gccccttcgt ggacgaaaac ggcagggagg agaccgaccg gcggtgggcc gaagtgaaca
     1141 tcgtgccgac ctcgctcgtt tcgagcgagc cctatccgga aatccgcttc atcgccgtgc
     1201 gggagaggat gaagcatccg aagctgaagg agagaatgga cgccgcgcag atggagctcg
     1261 cgatagaaga gatcgagggg gagcatccga agacggagcg actgcacctg acctgcatgg
     1321 acggaacggt ctggaaggtt ttcctgatgg tgaccaacga cttcgagcgg aacggacagc
     1381 acgcgctcga gcatcactgg aagcgatgcg ggaagtccga ggaggtgcac ggaatcctga
     1441 agaacgacct cgccggggga cacgtgccga gcgccgactt cggctcctgc gcggtctggt
     1501 ggtatctgtg cgtcctgtcg tacaaccttc agcggcttat gacgcatcgt ctctttccgg
     1561 aggagtgggc gcgcgcgcat cagaagcggt tgttggcgac gatctactcg acgcctgcga
     1621 agatggtgcg ccacggaaag cagatagtga tacgagtgtt cggtcttctt ggaagactga
     1681 tcgaagccgc gcattggaag gtgctgcggc tcggtcgcct gaatatttga tattcacgga
     1741 gggaggggtg atgcgatagc gaagacgccg ccgccgagtg gagcgcggcg cggatggagt
     1801 gtactttcat tgcgacaaac tcagcctttc tgtggataaa aatgaatgcg ggagggtgtt
     1861 tttcgacatc
//
LOCUS       DAOPKU010000593         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_73070_length_1869_cov_3.872106, whole genome shotgun sequence.
ACCESSION   DAOPKU010000593 DAOPKU010000000
VERSION     DAOPKU010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73070_length_1869_cov_3.872106"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..517
                     /locus_tag="PK849_13960"
     CDS             272..517
                     /locus_tag="PK849_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HPE67282.1"
                     /translation="MPLNELAVPNRVTGERQVRRTLAGGRLRKLFIAEDADPSVIKNL
                     EEEARRQNIPVERVDSMLRLGRACAITRGASAAGIEG"
     gene            611..982
                     /gene="rpsL"
                     /locus_tag="PK849_13965"
     CDS             611..982
                     /gene="rpsL"
                     /locus_tag="PK849_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPE67283.1"
                     /translation="MPTINQLIRHGREEKRRKSDSPALQGNPARRGVCTRVYTVTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIIRGTL
                     DCGGVENRKRSRSKYGARKPK"
     gene            1027..1497
                     /gene="rpsG"
                     /locus_tag="PK849_13970"
     CDS             1027..1497
                     /gene="rpsG"
                     /locus_tag="PK849_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPE67284.1"
                     /translation="MPRKGHVKKRQATPDTKFGSPALGKFINSLMRKGKKSVAERIMY
                     NALDIAGEKLQTEPFEVFKKAMENVRPLVEVRPRRVGGATYQVPVEVPAERAQVLSIR
                     WILNYSRSKKGMPMAERLAREFMDACKNEGASIKKKEDTHKMAEANRAFAHYRW"
     gene            1562..>1869
                     /locus_tag="PK849_13975"
     CDS             1562..>1869
                     /locus_tag="PK849_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPE67285.1"
                     /translation="MQKLDMKSIRNIGIAAHIDAGKTTTTERILFYTGKNYKMGETHE
                     GAATMDYMDQERKRGITITSAATTCIWHDHRINIIDTPGHVDFTVEVERSMRVLDGAV
                     A"
BASE COUNT          489 a          460 c          565 g          355 t
ORIGIN      
        1 ggagccgcag tgaagggaga gatcgatccc cttgaaggac tgaaggaaaa cgtcatcatc
       61 ggacatctca ttccggccgg aacaggagtt gaacgattcc agggggttcg cacatacatt
      121 cagcaccccg aaaaggaaga atagctcttc ggcgtctttg ctccagagag agaaaatgta
      181 aatactctga aggcgggagc attttatgct tgacattgct cccgcctttt tgttattatt
      241 ccgtggtgct tttggcacag gagggatttt attgccttta aatgaactcg cagtcccgaa
      301 cagagtgacg ggagaacgtc aggtacggcg gacgcttgcc ggcggacggc tccggaagct
      361 ctttatagcg gaggatgcgg acccttcagt gataaaaaac ctcgaagaag aagcgcggcg
      421 gcagaatatt ccggttgaac gcgtcgacag tatgctccgg ctcggaagag cctgcgccat
      481 cacacgagga gcttcggcgg ctggaataga aggatagatg ggcgaatatc cggaagacga
      541 gggaaaaggg tttttcggaa aaaagtttct ttgcccagaa tacgaagaac agatggaagg
      601 aggaagttgc gtgccaacga tcaatcagct tatacggcat ggccgggaag aaaagaggcg
      661 caagagcgat tcgcctgcgc tgcagggaaa ccccgcccgg cggggtgtct gcacgcgtgt
      721 gtacacggtg acgccgaaaa agcctaactc ggctctgcgg aaggttgccc gagtccgcct
      781 caccaacggt atcgaggtta cctcgtacat tccgggaatc ggccacaacc tgcaggagca
      841 ctcggtggtt cttgttcgtg gcggaagagt gaaagacctt cccggagtcc ggtatcacat
      901 tattcgggga acactcgact gtggaggcgt ggagaatcga aagagatccc gctccaagta
      961 cggcgcccgg aaacccaagt agggttttgc ggcgtgtttt ttgttatttt gaggaggggt
     1021 tgaccaatgc cgcgtaaggg gcatgtcaag aaacgccaag cgacgccgga taccaagttc
     1081 ggcagccccg cactgggtaa gttcatcaac agcctgatgc ggaagggaaa gaagagcgtg
     1141 gccgagcgga ttatgtacaa cgcgctcgat atcgccggag agaagcttca gacggagccc
     1201 ttcgaggtct tcaagaaggc catggagaac gtccgtcctc tcgtcgaggt tcgcccccgc
     1261 cgcgtcggcg gagcgaccta ccaggtaccg gtggaagtac ccgccgaaag agcgcaggtg
     1321 ctctccatca gatggatcct gaactattcc cggagcaaga agggaatgcc catggctgaa
     1381 agactcgcca gggagttcat ggatgcctgc aagaacgaag gcgcttccat caagaagaag
     1441 gaagatacgc acaagatggc ggaggctaac cgcgcgttcg cccattaccg ctggtaataa
     1501 agtcgtttac agattcaata cgatgcagtg ctctgcagga aacgaggctt ggtggtagga
     1561 aatgcaaaaa cttgatatga aaagtatccg taacattgga atcgccgccc atattgacgc
     1621 gggcaagacg acgaccactg agcgaattct tttctacacc ggaaaaaact acaaaatggg
     1681 cgaaacgcac gaaggcgccg ccaccatgga ctacatggac caggaacgga agcggggaat
     1741 caccatcacc tctgccgcga cgacgtgcat atggcacgat catcgtatca atattattga
     1801 tacgcccggg cacgtggatt tcaccgtgga ggtggagcgt tcgatgagag tgctcgacgg
     1861 cgcggtcgc
//
LOCUS       DAOPKU010000594         1865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_73287_length_1865_cov_4.544751, whole genome shotgun sequence.
ACCESSION   DAOPKU010000594 DAOPKU010000000
VERSION     DAOPKU010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73287_length_1865_cov_4.544751"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PK849_13980"
     CDS             <1..629
                     /locus_tag="PK849_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67286.1"
                     /translation="SVGILSFPSFELEGNNAALLFKHLGCTVYPIPLDSALLRRDLNA
                     LYVPHGPGFLCAERLLGKSGVREWFTSIFHARKAVLVNGGITPLLGESFSLPNRKEYE
                     GLKFFRFKGKFDIPSSDDVRRVEAVSTDGDVMLHQGDKIRGYLPPYISVLNPGNVAGA
                     VWSLQDTVKNMEIGLSGWSRGAALATDVCVELWSSIEGVYRWLMMRKQ"
     gene            complement(636..1496)
                     /locus_tag="PK849_13985"
     CDS             complement(636..1496)
                     /locus_tag="PK849_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67287.1"
                     /translation="MERPRCSTTHDIIVFDVDGVLVDTSRSYFEVIVQALDWAWTSLI
                     NGVVDCEGFSERHFATTKTHPAFNDDYDIAWAIINCAATAQNPSLAQSLPAPEEWRLL
                     LDGCKESDVPSWVRSTFGEQIPRDTVRRACEELYFGVTKNEKHTALLSPRKGLWENER
                     PMITIPWSAIPVPAGIYTGRTAKELELALKRLHWEDFPDSMIITSDSGVTKPSPRGLE
                     ILCHTSHASTPLFLGDTESDRQTVHAFGRGTFAAIGNFLSDAEFAFPCPEEALEYFGF
                     LSPSNSSVNL"
     gene            1577..>1865
                     /locus_tag="PK849_13990"
     CDS             1577..>1865
                     /locus_tag="PK849_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPE67288.1"
                     /translation="MLLEIPLNYSEGVLYRQLAEHLEKMIRTGSISSGERLPGTRELA
                     RMLRVSRCTVVAAYDVLEENGLVKRTARSGTFAASPLLEGRSSYKGKGDILR"
BASE COUNT          416 a          449 c          534 g          466 t
ORIGIN      
        1 tgagcgttgg aattctttct tttccctcgt ttgagctgga aggaaataac gctgcgttgc
       61 ttttcaagca cctcgggtgc acggtctacc cgattcccct tgacagtgcg cttcttcgcc
      121 gggacttgaa cgcactttac gttccgcacg gcccggggtt cctttgtgct gaacgactat
      181 tggggaagtc cggggttcgg gaatggttta catcgatttt tcacgcccgg aaggcagtgc
      241 tggttaatgg cggcattacg ccgcttcttg gggagtcgtt ttctttaccg aacagaaaag
      301 agtacgaggg gctcaagttc ttccgtttta agggaaagtt cgatatccct tcatccgatg
      361 acgtccgcag ggttgaggct gtttctaccg acggagatgt tatgctccat caaggagata
      421 aaatccgggg gtacctcccc ccctacatat ctgtgctgaa tccggggaat gtggccggag
      481 cagtgtggag tctccaggac acggtcaaaa atatggagat cgggctctcg ggatggagca
      541 ggggagccgc tttagctacc gacgtgtgcg tcgagctgtg gagcagtatt gaaggtgtgt
      601 atcggtggct gatgatgcgg aaacagtgac gttcttcata agtttacact gctgttggat
      661 ggagagagaa aaccgaaata ttccaaggcc tcctcagggc agggaaatgc aaactccgca
      721 tcggagagga aattcccgat ggcggcaaat gttcctctgc cgaaagcgtg cacggtctgc
      781 cggtcgcttt ccgtatctcc aagaaagagc ggcgtcgatg cgtgagaggt atggcacaga
      841 atctcaagtc cgcgaggaga tggttttgta actccggagt cggaagtgat tatcatggaa
      901 tccggaaaat cctcccagtg aagacgcttg agtgcaagtt ccagctcttt ggcggttcgt
      961 cctgtataaa ttcctgcagg gacaggaatc gcgctccacg gaatcgtgat catcggacgc
     1021 tcgttttccc atagtccttt acggggcgag agcaacgcag tgtgcttttc gtttttcgtg
     1081 acgccgaaat aaagttcttc gcatgctctg cgtacagtat ccctgggaat ttgttccccg
     1141 aacgtactcc gtacccagga aggcacgtca gattccttgc atccgtcgag caagagtctc
     1201 cattcttcag gcgcggggag actttgtgca agagagggat tctgcgccgt tgcagcgcag
     1261 ttgatgattg cccaggcaat atcgtagtcg tcattgaacg ccgggtgtgt tttggtggtt
     1321 gcgaagtgac gttcagaaaa accttcgcaa tccacgaccc catttataag cgacgtccat
     1381 gcccagtcga gagcctggac gatcacctcg aaataggacc gggatgtgtc gacaagcacg
     1441 ccgtcgacat caaaaactat aatatcgtgt gtcgttgaac atcttgggcg ttccatcgca
     1501 aacgctcctt ttcaagtatt tttctcccga agtattgtac cagatgaacc ggcgcagatg
     1561 gaaaggagag ggtgttttgc tgctggaaat accgttgaac tactctgaag gagtactcta
     1621 ccgtcagctc gccgaacatt tggagaagat gatacgaacc ggaagcatct cttccggcga
     1681 gcgtcttccg gggacacggg agcttgccag gatgctccgg gtaagccgtt gtactgtcgt
     1741 tgccgcctac gatgtcctcg aagagaacgg tctggtgaag agaacggcga gaagcgggac
     1801 ttttgctgct tctcctcttc ttgaagggcg gtcttcttat aaaggcaaag gggatattct
     1861 ccgtt
//
LOCUS       DAOPKU010000595         1861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_73516_length_1861_cov_5.296235, whole genome shotgun sequence.
ACCESSION   DAOPKU010000595 DAOPKU010000000
VERSION     DAOPKU010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73516_length_1861_cov_5.296235"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="PK849_13995"
     CDS             <1..1332
                     /locus_tag="PK849_13995"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPE67289.1"
                     /translation="AAELYSVASGNGPLALRGLAVRGKISTRSANAAVTDSAAAGTAL
                     ATGFKTNNGVLGMDPSGMKKLPTVAEVARDRGMKVGVVTSVFLNDATPAAFYAARPSR
                     TDFYEIGVQMVESGFDYFAGGGVNRRAGRKGDLKDVYDLAREKGYRVLRTREELVAAR
                     PGTKIIAAASVGGALPYETDRSAGSPSLAEFTKRGIELLDNPDGFFMMVEGGRIDWAC
                     HRNDAAAAVRDVLAFDEAVKEALAFARARPNDTLIVVLADHETGGMRVDAERAAANLF
                     PALSAQRESSESFDEKLERFRKGTARTFERMAPLLAESFGLHATSSAGRAALDARAKK
                     GDGAARTALLSALSGEELDELRTAFAQSMRAKEQRKKDRDYLRRYGPYEPLSVTATRI
                     LNRKAGVEWSSFAHTGADVPVFAQGAGAARFAGEYDNTNVAEKILSVLGKR"
     gene            1583..>1861
                     /locus_tag="PK849_14000"
     CDS             1583..>1861
                     /locus_tag="PK849_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPE67290.1"
                     /translation="MKRSIVSDETRNKEAKKATWTGFAVNALLTFFKLFAGFRGGSAA
                     MIADGVHSLSDFFTDIVVLVGFKFTEKPADDEHNYGHGKYETLATVVIS"
BASE COUNT          350 a          568 c          634 g          309 t
ORIGIN      
        1 gccgctgagc tgtacagcgt cgcgtccgga aacgggccgc tcgcgctgcg cggacttgcc
       61 gtgcgcggga agatctccac gcggtcggcg aacgccgccg tgacggactc cgccgccgcc
      121 gggacggcgc tggccaccgg attcaagacg aacaacggcg tcctcggcat ggatccttcg
      181 gggatgaaaa aactgcccac cgtcgcggaa gtcgcccgcg accgggggat gaaggtcggc
      241 gtggtcacgt cggtctttct gaacgacgcc actccggcgg ccttctacgc cgcgcgtccc
      301 tcccggaccg acttttacga gatcggcgtc cagatggtcg agagcggctt cgactacttc
      361 gcggggggcg gcgtcaaccg gcgcgcgggg cgaaagggcg acctgaagga cgtgtacgac
      421 ctcgcccgcg agaaggggta ccgcgtgctc cgcacccgcg aggagcttgt cgcggcgagg
      481 ccggggacaa aaatcatcgc ggccgcgtcg gtcggcgggg cgctgccgta cgagacggac
      541 cggtctgcgg ggagcccctc ccttgcggag ttcacgaagc ggggtatcga gctgctcgac
      601 aacccggacg gcttcttcat gatggtcgag ggggggagga tcgactgggc atgtcatcgc
      661 aacgacgcgg cggcggccgt tcgcgacgtg ctggccttcg acgaggcggt gaaggaggcg
      721 ctggccttcg cccgcgcccg cccgaacgat acgctgatcg tcgtcctcgc cgaccacgag
      781 acgggcggca tgcgcgtcga cgcggagcgc gcggcggcga atctcttccc ggcgctctcg
      841 gcgcagaggg agtcctccga gtctttcgac gagaagctcg agcgcttccg caaggggacg
      901 gcgcggacgt tcgagcgcat ggcgccgctg ctcgcggagt ccttcggcct ccacgccacc
      961 tcctccgccg ggcgcgccgc gcttgatgcc agggcgaaga agggcgacgg ggccgcgcgg
     1021 acggcgcttc tgtccgcgct ctcgggcgaa gagctggacg agctgcggac ggccttcgcg
     1081 cagagcatgc gggccaagga gcagcggaag aaggacaggg actatctgcg ccgctacggc
     1141 ccctacgaac cgctttccgt gaccgctacg cgcattctga accggaaggc gggggtagag
     1201 tggtcttcct tcgcccacac aggggccgac gtccccgtct tcgcacaggg cgcgggcgcg
     1261 gcacgcttcg ccggggagta cgacaataca aacgtcgccg aaaagatcct gtccgttctc
     1321 ggcaaacgct agacgaaccg gcaatccgaa actccgaggg gcggacgggg actttctccg
     1381 gggctccttg tgaaccggaa cgcatacgac ggctcgcaac acattgacaa gtctattcac
     1441 tctttatata ctaaaacggc atcaggtgtt gcatactgtg cagcagccgg gccgaaaggc
     1501 agcaggtacg acctgtggga ctgtgatcgt cattcatggt tccacagttt tttattcgta
     1561 gagaacgaag gagcgtatcg ctatgaagcg gagtatcgtc tcggacgaaa cgagaaacaa
     1621 agaggccaaa aaggcgacct ggacgggctt tgccgtgaac gcattgttga cgttcttcaa
     1681 actcttcgcc ggattcagag gcggaagcgc cgcgatgatt gcggacgggg tgcactctct
     1741 gtcggatttt tttacggaca tcgtggtgct ggtcggtttt aaatttacgg agaaacccgc
     1801 cgatgatgaa cacaactacg gacatggaaa atatgaaacg cttgcgacgg tcgtcatcag
     1861 t
//
LOCUS       DAOPKU010000596         1853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_73951_length_1853_cov_5.109566, whole genome shotgun sequence.
ACCESSION   DAOPKU010000596 DAOPKU010000000
VERSION     DAOPKU010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73951_length_1853_cov_5.109566"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PK849_14005"
     CDS             complement(<1..272)
                     /locus_tag="PK849_14005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HPE67291.1"
                     /translation="MSIESAAAFYDRLQNDDALQEQLKAAGDRGVMEAYVRDKLGYDF
                     TKEEMQKVIFERNPEMSDEDLEAVVGGVSDDMLWGITIGVGVGGGMG"
     gene            complement(403..1722)
                     /locus_tag="PK849_14010"
     CDS             complement(403..1722)
                     /locus_tag="PK849_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67292.1"
                     /translation="MRKYRGMPMVLVVLLMTAVMFGGGCGGGSDRMPAHPGEIQPGAE
                     YEGVLSVQDGDEDRVLMRTRYEAHNIETGDSMEPVVALFVKQEEHLDEATGTMRVLSA
                     SEISRPTPEQVKEFLKDFWDAEHGGDADEGVNAREFLDFLAAHNMSLDQFFRYFEALD
                     SSVSAFIDVIETYSTKLSSFDGKDIYGETESFCDLLGLELREYFQKIILVFGTHAAYC
                     DKLVMLGGIGKSTLYDAYSKWLLDQEVAPEDPFVGFLNYLKARSAEASGARPGGAAET
                     ALEVAKFAWDIIKDGKAQVTTDGAFTSVLRKDTTALDYGYAKRNETSIIHFKITDAWI
                     KSWVLIETKFRGSASYDAVNPTFGGKYLQNVQFDVESAYAQWGMYLNVSAQVSNVTNP
                     ASVENPDPEIDVVARINAGWLFQSFGRSAYFRAKGSQGIWFQRWGDN"
BASE COUNT          393 a          594 c          450 g          416 t
ORIGIN      
        1 cccatccccc cgccgacgcc gacgccgata gtaatccccc acagcatatc gtcgcttact
       61 cctccgacga cggcctccag atcttcgtcg ctcatctccg ggttccgttc gaaaatcacc
      121 ttctgcatct cttcctttgt gaagtcatac ccgagtttgt cccgcacgta tgcttccatc
      181 acgcccctgt cgcccgccgc tttcagttgt tcctgaagcg cgtcgtcgtt ctggagacgg
      241 tcgtaaaacg cagcagcaga ttcaatcgac atggctcttc ctcctttttc gtacagtccg
      301 gaaacgaaaa gagggggaaa tgcgtcgttc cccctctttt tcagtcattg catcgttttc
      361 aacccggtat caaacgcatg caaccttctc cgcgttctta tctcaattgt cgccccagcg
      421 ctggaaccag atcccctggc ttcccttggc cctgaagtac gccgatctgc cgaacgactg
      481 gaagagccac ccggcgttga tgcgcgcgac gacgtcgatc tcggggtcgg ggttctccac
      541 cgatgcgggg ttcgtcacgt tggagacctg cgcgctcacg ttgaggtaca ttccccactg
      601 ggcgtacgcg ctctccacgt cgaactggac gttctgcagg tacttcccgc cgaaagtagg
      661 attgaccgcg tcgtacgaag cggaaccgcg gaatttggtc tcgatgagca cccacgactt
      721 gatccatgcg tcggttattt taaagtggat gatcgacgtc tcgttgcgtt tcgcgtagcc
      781 gtaatcaaga gcggtcgtgt ctttcctcag cacggacgta aaggcgccgt cggtagtgac
      841 ctgtgccttt ccgtccttga tgatatccca cgcgaactta gcaacttcca gggcggtttc
      901 ggccgcgccg ccggggcgag cgccggaggc ttccgccgaa cgcgccttca ggtagttcag
      961 gaacccaaca aacgggtctt ccggtgcgac ctcctgatcc aataaccatt ttgaatacgc
     1021 gtcgtacaga gtggacttcc ctatcccccc gagcatgacc agcttgtcgc agtaggccgc
     1081 atgcgttccg aaaaccagga taattttttg aaaatattcc cgcagctcca gaccgaggag
     1141 atcgcagaaa gactcggtct ccccgtatat gtctttgccg tcgaaagagg acagcttcgt
     1201 tgagtacgtc tcgataacat ctataaaagc cgaaacggaa gagtccagcg cctcaaaata
     1261 gcggaagaac tgatcaaggg acatgttatg cgcggcaaga aaatccagaa attcgcgcgc
     1321 attgacgcct tcatccgcat cgccgccgtg ctccgcgtcc cagaagtcct tcaggaactc
     1381 ctttacttgt tcgggagtcg gcctcgagat ttcggacgcg gacagaacgc gcattgtgcc
     1441 cgtcgcctcg tcaaggtgct cttcctgctt tacgaaaagc gcaaccaccg gctccatcga
     1501 atcgccggtc tcgatattat gcgcttcata tcgggtgcgc atgagcaccc ggtcctcgtc
     1561 gccgtcctga accgaaagca caccctcgta ttccgctccc ggctgaatct ccccaggatg
     1621 cgccggcatt ctgtccgacc cgccgccgca tccccctccg aacatcaccg ccgtcatcaa
     1681 gagcactacc aacaccatgg gcattccgcg gtatttcctc atgagcaacc actcctttca
     1741 atcaaaataa ccagcgattc acacacatgc tctcttcttc gggaaaacga agaggaatcc
     1801 gagttacaga cctgccgtaa cgatcacatt tgcaacagaa agcccgtcag atc
//
LOCUS       DAOPKU010000597         1849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_74212_length_1849_cov_4.346154, whole genome shotgun sequence.
ACCESSION   DAOPKU010000597 DAOPKU010000000
VERSION     DAOPKU010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74212_length_1849_cov_4.346154"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PK849_14015"
     CDS             <1..441
                     /locus_tag="PK849_14015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPE67293.1"
                     /translation="RSDAFSSIGTMVGIGGAIFLGRNWAFLDPLASLIVSVFIFKVSF
                     EILMPAVNELLESSLQDEEIAYIKKIIEASPHIQNYHHLRTRRIGAKVAIEVHFVFEK
                     TMSIREAHSYATEVEKKIQEYFGASSLITTHLEPSRDAQEATRA"
     gene            638..>1849
                     /locus_tag="PK849_14020"
     CDS             638..>1849
                     /locus_tag="PK849_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPE67294.1"
                     /translation="MKRSLWNKIGLTLLALLVLAGAACAAGVNNDWGGAKAKYVFMFI
                     GDGMGLQQVNAAEVYNGSIDRTGGEPAIKKLALSGLPVQGMITTYSTNSFITDSAPAA
                     TSLASGYKTDNGVIGMDPAKKQKFTTVAQMVRAKGMKVGVLSSVSLNHATPASFYASV
                     PSRNDYYGIGEQLLDSGFDYFAGGGIHQSTGKEKDRKDLYEVAKEKGYQVLRTREEIL
                     AAKPGTKVIAVNPVLDGSNAMPYDMDRTPEQLSLAEFTKKGIELLDNPNGFFMMVEGG
                     KIDWACHANDAAATILDTFAFDAAVMEALSFAEKHPDETLIVVVGDHETGGMSIGFAG
                     TQYETFFEKIAHQKESYESFDKKIAAFRKGEEKTFEKMAPMIEESFGLRTATAEERAA
                     LEEKAKAGDDAA"
BASE COUNT          407 a          530 c          578 g          334 t
ORIGIN      
        1 cggtcggatg cgttttcctc cataggaacg atggtcggaa tcggaggagc gatattttta
       61 ggtcgaaact gggcctttct tgatcctctg gcgtctctga tcgtcagcgt gtttattttc
      121 aaggtgtctt tcgagatttt gatgccggcc gtcaatgaac tgctggagtc ctctcttcag
      181 gatgaagaga tcgcatacat caaaaaaatc atcgaagcct cgccgcatat tcaaaattat
      241 caccatctgc gaacgagacg gatcggggcg aaggttgcca ttgaagttca cttcgttttt
      301 gaaaaaacca tgtcgattcg cgaggcgcat tcctatgcaa ccgaagtgga aaaaaagata
      361 caagagtatt tcggcgcttc cagtcttata acgacgcatc tggagccgag ccgcgacgcg
      421 caggaagcga cgcgggcata agcgggaagg ggctgcggag acggacgcgc cgtatatacg
      481 gacatcttcc cggtgcgttc tctgcgggat actgaggccg gaggaggcgc ggcgaccgcg
      541 cgccgagcga gttttaccct cccgatacac ttcttcgctt cttccgttac cctccttgaa
      601 gataattggc tcaaattctt cgaggagggt aaaaaatatg aagcgttcac tgtggaacaa
      661 gatcggattg acgctgctcg cgctgctggt gctggcaggg gcggcctgtg ctgcgggggt
      721 caacaacgac tggggcggcg cgaaggcgaa gtacgtgttc atgttcatcg gcgacggcat
      781 ggggttgcag caggtcaacg cggcggaggt ctacaacggc tccatcgaca ggacgggcgg
      841 cgagcccgcg atcaagaagc tcgccctgag cggccttccg gtgcagggga tgatcaccac
      901 ctactccacc aactccttca tcaccgactc ggccccggcg gcaacctcgc tggcctccgg
      961 ctacaagacg gacaacggcg ttatcggcat ggacccggcc aagaaacaga agttcaccac
     1021 cgtggcccag atggtccgcg ccaaggggat gaaggtcggc gtgctcagct ccgtctcgct
     1081 gaaccacgcg acccctgcgt ccttctacgc ctccgttcct tcacgcaacg actactacgg
     1141 tatcggcgag cagcttctcg acagcggctt cgactacttc gcgggcggcg gcatccatca
     1201 gagcaccggc aaggagaagg accgcaaaga cctctacgag gtcgcgaagg agaaggggta
     1261 tcaggtgctc cgcacccgcg aggagattct cgcggcgaag ccgggaacga aggtcatcgc
     1321 ggtgaacccc gtgctcgacg gcagcaacgc catgccctac gacatggaca ggacgccgga
     1381 gcagctctcc ctcgccgagt tcacgaagaa gggcatcgag ctgctggaca acccgaacgg
     1441 attcttcatg atggtcgagg gcggcaagat cgactgggcc tgtcacgcga acgacgccgc
     1501 cgcgaccatt ctggacacct tcgccttcga cgcggcggtg atggaggccc tgagcttcgc
     1561 ggagaagcac cccgacgaga cgctgatcgt cgtcgtgggc gaccacgaga cgggcggcat
     1621 gagcatcggc ttcgccggga cgcagtacga gaccttcttc gagaagatcg cgcaccagaa
     1681 ggagtcgtac gagtcgttcg acaagaagat tgccgcgttc cgcaagggtg aagaaaagac
     1741 gttcgagaag atggcgccga tgatcgagga gtcctttggc ctccgcacgg cgacggccga
     1801 ggagcgcgcc gcgctggaag agaaggcgaa ggccggcgac gacgcggcc
//
LOCUS       DAOPKU010000598         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_74494_length_1844_cov_5.702627, whole genome shotgun sequence.
ACCESSION   DAOPKU010000598 DAOPKU010000000
VERSION     DAOPKU010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74494_length_1844_cov_5.702627"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..>1844
                     /locus_tag="PK849_14025"
     CDS             136..>1844
                     /locus_tag="PK849_14025"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HPE67295.1"
                     /translation="MNTKKTGSAKAATETIALESINVLTPGSAPVALKLARIGGIGET
                     VDGSVSWDRSRCSLSPGIVAETLVSALLCGCRALYNMKSFWEESGCAAWFERNGVVPE
                     QLNDDVYARLLDRFSECDMRGIVETVAFRLRTAHGIKVSTVHFDTTSVSVEGDYATTK
                     EERGEESDGAETSEPAAADAEKPSFEIERGHSKDRRPDLKQIKIGLAVQEDGIPLTGE
                     LLSGSASDRTWNGDAVGYISELLGGQGVEDAVFVADSALVSKENLAELADGGMDFVTL
                     LPGTFSLEKELKERAWREEKWTDLGALARDPDEKSARYKTRSVREEIEGRTYDFLVVH
                     STKLEALKERSLKSRFDRLGVELRKESLALRKREFACEEDARRGGAELLEKAAKYGIV
                     SSVSVEFEEKAVRGKGRPKKDAPPPATNRTWRAVVEIGEVEEKTWKSAMDRESTFILV
                     YRMEKAVERKNPADILRTYKNQNVVEQGFRFLKQPIYLGPVLLKKPERVEALGYVFLL
                     VLLLAKYLEYRVRASLEKEGDALRVGGQKLARPTTQTILYHFGTVPLLESGGKRFMPN
                     PPGG"
BASE COUNT          412 a          487 c          619 g          326 t
ORIGIN      
        1 attgtccgcc gtttcatcat catctaaact cttcttacgc tcggagtgta ttccgtattt
       61 cgcatgtcga tttgaatgat cgggtatttt ttgaggcagg tgacaatgtg tcgtatttgc
      121 cataaaatag gtcgtatgaa tacaaaaaag actggcagcg caaaagctgc aacggagaca
      181 atcgcgcttg aatccataaa tgtgctcacc cccggctccg ctcccgtcgc tctgaaactc
      241 gcgcgcatag gaggaatcgg agaaacggtg gacgggagcg tgtcgtggga ccgttcccga
      301 tgctccctct ctcccggaat agtggcagaa acgctcgtat cggctcttct ttgcggctgt
      361 cgggcgctct acaacatgaa gtccttctgg gaggagtccg gatgcgccgc ctggttcgag
      421 aggaacggcg tcgtgccgga gcagctgaac gacgatgtgt acgcgcggct tctggacagg
      481 ttctccgaat gcgacatgcg cggaatagtg gagaccgtgg cgttcaggct caggacggcg
      541 cacgggatca aggtttcgac cgtgcatttc gacacgacgt cggtctccgt ggaaggcgac
      601 tatgcgacga cgaaggaaga gcgcggagag gagagcgacg gcgcggagac gtccgaaccc
      661 gccgccgcgg acgccgaaaa gccctctttc gagatcgagc gcgggcactc caaggaccgt
      721 cgtccggacc tgaagcagat caagatcggc ctcgcggtgc aggaggatgg gattccgctc
      781 acgggagaac ttctctccgg cagcgcttcg gaccggacgt ggaacggaga cgccgtcggg
      841 tacatatcgg aactgctcgg agggcaggga gtcgaagacg ccgttttcgt ggccgacagc
      901 gcgctcgtga gcaaggagaa cctggcggag ctcgccgacg gaggaatgga tttcgtgacg
      961 ctgcttcccg ggacattttc tctggagaag gagctcaagg agagagcgtg gcgcgaagag
     1021 aagtggacgg acctcggcgc tctcgcccgg gatcccgacg aaaagagcgc gcgctacaag
     1081 acgcggagcg tccgggagga gatcgaggga agaacgtacg actttctcgt ggtccactcg
     1141 acgaagctcg aagccttgaa ggagaggagt ctgaaaagcc gtttcgacag actgggcgtc
     1201 gaactgcgca aagagagtct cgcactgcgg aagcgggagt tcgcctgcga ggaggacgcc
     1261 cgaagaggcg gagcggaact tctggagaag gcggcgaagt atgggatcgt ctcctccgtc
     1321 tccgtcgagt tcgaggagaa agccgtacgg ggcaagggca gaccgaagaa ggacgctccc
     1381 cctccggcga cgaaccgtac gtggagggcg gtcgtcgaaa taggagaggt cgaggagaaa
     1441 acgtggaaat ccgccatgga cagggagtcg acattcattc tcgtgtatcg gatggagaag
     1501 gctgtcgagc ggaagaatcc cgcggacatc ctgaggacgt acaagaacca gaacgtcgtg
     1561 gaacagggat tccgcttcct caagcagccg atctacctcg gccccgtcct gctgaagaag
     1621 ccggaacggg tggaagcgct gggctacgtc tttcttctcg tgctgcttct cgcgaagtat
     1681 cttgaatacc gggtacgggc gtcgctggag aaggaaggcg acgcgctgcg ggtcggagga
     1741 cagaaactcg cccgtcctac gacgcagacg atcctgtacc atttcggaac cgtgccgctg
     1801 ctggagagcg gagggaaacg gttcatgccg aatccgcccg gagg
//
LOCUS       DAOPKU010000599         1839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_74802_length_1839_cov_4.502803, whole genome shotgun sequence.
ACCESSION   DAOPKU010000599 DAOPKU010000000
VERSION     DAOPKU010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74802_length_1839_cov_4.502803"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..720
                     /locus_tag="PK849_14030"
     CDS             295..720
                     /locus_tag="PK849_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HPE67296.1"
                     /translation="MAKIIALCTSSQRQEPKSEVAEAHCVHGGIEGDAHRGVTEREIS
                     LLRAEDIRRAEQEAGFVFPPGSLAENLVVEGLPEDLAPGARLSIGPEAVLEVVEKGKK
                     PGEPHSYSYKGWCLLPTAGFFLRVIEEGVVSKGDEVESL"
     gene            801..>1839
                     /locus_tag="PK849_14035"
     CDS             801..>1839
                     /locus_tag="PK849_14035"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HPE67297.1"
                     /translation="MFRKMKIGGKLSLAFGVLLLIFAGVGAMSWMNMSGVQQEARSLA
                     ERYVPELVVAANVQNTVQTMMYEVRGYNFTYSKSYLDAGRNAVVEAQKALKEASDLGE
                     KYPTLVALREGAAKAVAALDEYVVLIDRTETAVNAIDGARTSGNESARLFMENAEAYL
                     ASQNQAMLHALRNNEPREETEDRLQKITLANEIIDAGNAVRLANYMGQATRDVVRFEE
                     GMKNFDEIDSLVKKLRADTAQKVNLDQLDAVQKAAGEYKTAMEKTLASWRELDEINTR
                     RVETGRVILTLADQVVHAAETQSMKITGQAVSSLGSTILVILIATVVAVLFGGIVAFL
                     MTRSLTVPLKRV"
BASE COUNT          405 a          490 c          593 g          351 t
ORIGIN      
        1 cggcggttgc gccggagcgg cgccgttcgc gaggtcgatt tcaagctgcc ggcgaggtga
       61 tgtgaaggtt tttcgcaaga gccggacgct cgaagcccga tgtccgaagc ccgagaggac
      121 gtcctccggg tgtatcgcaa tgatattgcg gagaaaaagc ggtcttgcca aaaaggtaag
      181 gccgcttttg agtcttttcc gggtgggcag ggggtcgtat ttttcccttt catcttgtat
      241 tattcaatag agtcaatgtt tcgttatcct gttgccgatg taggaatggt tattttggcg
      301 aagatcattg cactgtgtac aagttcgcag cgacaggaac cgaagagcga ggttgcggag
      361 gcgcattgtg ttcatggagg catcgaagga gatgctcaca gaggagtgac cgagagagag
      421 atcagccttc ttcgcgccga ggacatccgg agggcggagc aggaggccgg atttgtcttc
      481 ccccccggat cgcttgcgga gaatctcgtt gtggaagggc ttccggagga tcttgcgccg
      541 ggagcgcgtc tctcgatcgg ccccgaggcc gtcctcgaag tcgtggagaa gggaaaaaag
      601 ccgggcgagc cgcacagcta cagttacaaa gggtggtgtc tgctgccgac ggcggggttc
      661 tttcttcgcg tgatcgagga aggtgttgtc tcgaaggggg acgaagtcga atccctttga
      721 agcgcgtgcc tttctgaatg agaaaccgtt ggatctgaaa caatcgtatt cattatttca
      781 cggaaggaga ggtggatgca gtgtttcgaa agatgaaaat cggagggaaa ctctcgctcg
      841 cgttcggcgt tctgctgctc atattcgcgg gagtcggcgc gatgagctgg atgaacatga
      901 gcggcgtgca acaggaagcc cgctctctcg cggagcggta cgttcctgaa ctggttgtcg
      961 ctgccaacgt gcagaatact gtgcagacca tgatgtacga agtccgagga tataacttca
     1021 cctactccaa aagctacctc gacgcggggc ggaacgctgt cgtcgaagcg cagaaggcgc
     1081 tgaaagaggc gtccgacctc ggggagaagt acccgacgct cgtcgcgctc cgggagggtg
     1141 cggcgaaggc cgtcgcggcg cttgacgagt acgtcgtcct gatcgacagg acggagacgg
     1201 cggtgaacgc catagacggt gcgcgaacca gcgggaacga aagcgcgcgc ctcttcatgg
     1261 agaacgccga ggcctacctt gcgtcccaga accaggccat gctccacgct ctccggaaca
     1321 acgaaccgcg cgaggaaacg gaagacaggc tgcagaagat cacccttgca aacgagatca
     1381 tcgacgcagg gaacgccgtg cgccttgcga actacatggg gcaggcgacc agggacgtcg
     1441 tccgattcga ggaggggatg aaaaatttcg acgaaataga ttccctcgtc aagaagctcc
     1501 gcgccgacac ggcgcagaag gtcaacctgg atcagctcga cgccgtgcag aaggctgcgg
     1561 gcgagtacaa aacggcaatg gagaaaactc tcgcgagctg gagggaactg gatgaaatca
     1621 acacccgccg cgtcgaaacc ggccgcgtca ttctgactct cgccgaccag gtggtccacg
     1681 ccgccgagac tcagtcgatg aagattaccg gccaggccgt ctccagtctc ggctccacca
     1741 tcctcgtcat cctgatcgcc acggtcgtcg cggtgctctt cggaggcatc gtcgccttcc
     1801 tcatgacgcg cagcctcaca gttccgctga agcgcgtgg
//
LOCUS       DAOPKU010000600         1835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_75068_length_1835_cov_2.447191, whole genome shotgun sequence.
ACCESSION   DAOPKU010000600 DAOPKU010000000
VERSION     DAOPKU010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75068_length_1835_cov_2.447191"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1835
                     /locus_tag="PK849_14040"
     CDS             <1..>1835
                     /locus_tag="PK849_14040"
                     /inference="COORDINATES: protein motif:HMM:NF037424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67298.1"
                     /translation="LTTPGDMEDRLQAGAYRVAYNLTPYVANVFTSGYRAVATDFLLN
                     SFLTMPTYLDAFKNSRNPEEFLSMVIPQAIIDLGMAWNTRGSPETHRRAVINRQVDEF
                     AKAAGISKEDARFFVENDWNRADEVMGRNFWEGTSPEVIREGDVKYDRYTGEKYEITS
                     VRPGSVFARLVGTGREEAIATRIGEIQRIFSDKLSGVLDDDTQIADASKLYAVAAMHA
                     EQVGAVKDALADAKAVTFERSEDGRVVTFYQNAGEGAKTALLKDLGRAKIMLSRGSRT
                     PEEIFRLTGWEKGFDGEWRFTIDDTKATFQPGLLSRGNVVDGLRDKLSKNVSVRLGDV
                     LNHPALFAAYPHLADMNIYLTEGKYGSKIAGKWSDDLPGIHIGTMASFDEGHNRQKTD
                     FEFMSALIHEIQHSIQNSERGFQHGFGMEGVRARIGIVQDALYKAHDAGDKDAENRYF
                     FELWRLGVPENEIGLEPSVKVLYDAYRRTAGEAEARAAQLRLTYTEEQLRNESPNETL
                     RKMLVEEGLHKEGDSILDTLLFLPRDPGDPDIAWSIPESAFQRGETRYQGGERSAFQD
                     SIDADYLAAVEAGDMKTAQKLVLEAARAAGYLSDSDYRMQHRAPN"
BASE COUNT          418 a          541 c          561 g          315 t
ORIGIN      
        1 ctcaccaccc ccggcgatat ggaggatcgc cttcaggcgg gggcgtaccg cgtggcctac
       61 aatctcaccc cttacgtggc gaacgtcttc acctcggggt accgggccgt tgccacggac
      121 ttccttctca actccttcct caccatgccc acgtacctcg atgcattcaa gaattcgcgc
      181 aaccctgagg aatttttatc catggttatt cctcaggcta tcattgacct tggcatggcg
      241 tggaacacaa gaggaagtcc agagacccat cgtcgcgctg ttatcaatcg tcaggttgac
      301 gagtttgcca aggccgcagg aatttctaag gaggatgctc ggttctttgt cgaaaacgac
      361 tggaatcggg cggacgaggt tatgggccgt aacttctggg aaggcacttc ccctgaagtt
      421 atcagggaag gcgacgtgaa gtatgaccgc tacacgggcg agaaatacga aatcacatca
      481 gtgcgccccg gctcagtctt tgccaggctc gtcggtacgg gccgtgaaga ggccatagca
      541 acaaggatcg gagagataca gcgcatcttc agcgacaaac tctccggcgt gctggacgac
      601 gacacccaga tagcggacgc ctcaaaactc tacgccgtgg ccgccatgca cgccgagcag
      661 gtgggggcag tgaaggacgc tctcgccgac gcgaaggccg tcacgttcga gcggagcgag
      721 gatggccgcg tggtgacatt ctaccagaac gccggggaag gggcgaagac cgctctgctg
      781 aaagaccttg gcagggcgaa gatcatgctc tccagaggca gtcgcacgcc agaggaaatc
      841 ttccgcctta ccgggtggga gaaggggttt gacggagagt ggcggttcac catcgacgac
      901 acaaaggcca cgttccagcc tgggctcctc tccaggggca acgtggtgga cggcctcagg
      961 gacaaactgt ctaaaaacgt gtccgtgagg ctcggcgacg ttctcaacca ccctgcgctc
     1021 ttcgccgcat acccccacct cgccgacatg aacatctacc tgactgaagg gaagtacggc
     1081 tcgaagattg cggggaagtg gagcgacgat cttcccggca tccacatcgg caccatggcg
     1141 agtttcgatg aggggcacaa caggcagaag acggacttcg agttcatgtc ggcactgatc
     1201 cacgagatac agcactccat ccagaacagc gagcggggtt tccagcatgg gttcggcatg
     1261 gaaggcgttc gcgcccgcat cggcatcgtc caggatgctc tgtacaaagc gcacgatgcc
     1321 ggagacaagg acgccgagaa caggtacttc ttcgagctgt ggcgcctcgg cgttccggag
     1381 aatgaaatcg ggctcgaacc gtccgtaaaa gtgctttacg acgcatatag gcggacggct
     1441 ggcgaggccg aggcccgtgc agctcagttg cgcctgacgt acaccgagga acagcttcgc
     1501 aacgagagcc ccaacgagac tcttcggaaa atgctcgtgg aagaggggtt gcacaaggaa
     1561 ggggacagca tactggatac cctcctgttc ctgcccagag accccggaga cccggacatc
     1621 gcatggagca tccccgagag cgccttccag cgcggggaga cccgctacca gggcggcgag
     1681 cggtccgctt tccaggacag tatcgacgct gactatctcg ccgccgtcga agcgggcgat
     1741 atgaaaacag cccaaaagct cgtgctggag gccgccagag cggcggggta tctcagcgac
     1801 agcgactacc ggatgcagca ccgggccccg aacag
//
LOCUS       DAOPKU010000601         1833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_75152_length_1833_cov_4.762092, whole genome shotgun sequence.
ACCESSION   DAOPKU010000601 DAOPKU010000000
VERSION     DAOPKU010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75152_length_1833_cov_4.762092"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PK849_14045"
     CDS             <1..893
                     /locus_tag="PK849_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE67299.1"
                     /translation="DKAFATAVVSVSAGLAIIIPPSISFIVYGDIMQQSVGTLFAAGV
                     VPGIVVAVFLCVTVWLISRKHGYRGAPRGTSKEVLFALKDAFWGLFTPVIILGGIYGG
                     VFTPTEAAAVAVFYGLFVGVFIYRSLTLRILYDILRETVAGTSVVMIVVTCAGLYSWL
                     GATVGLIDKIAALLLGISENPLVILLMINIILLFAGMLLDAISIAYVFLPILAPVIVT
                     FGWDPIWFGVMMTVNMAIGQVTPPVAVNLYVGARISGLTMEQITRPVIPLLVASIIAL
                     AVITVFPGITLYLPRLLGMM"
     gene            964..>1833
                     /locus_tag="PK849_14050"
     CDS             964..>1833
                     /locus_tag="PK849_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPE67300.1"
                     /translation="MASPVTMPKLGLTMNTGVVSQWKKKEGEPVRKGEILMVVATDKL
                     TFDVESPSEGVLLKVLVPEGKDVPIGEILAYIGAAGETVAGAAPEMPAAAPAAEEKKA
                     AAPSEVRSAPAGGIRATPLARKTAREAGIDLSLVAGSGPSGRIVRKDVDAAAASGGGA
                     VKASPVAAKMAAELGVDMASMRKDGRVMKEDVLRAAAVPEAEDVRIPLTPMRKVIAER
                     MSLSNSTVPSVTYTLEADFSALVAFRNKVKDEAAKKGVKISYNHILMKICAAALKENP
                     LANASFEGDAIVMH"
BASE COUNT          323 a          551 c          576 g          383 t
ORIGIN      
        1 atgacaaggc gttcgccacc gcggtcgtct ccgtgtccgc ggggcttgcg atcatcattc
       61 cgccgagtat ctccttcatc gtgtacggcg acatcatgca gcagtcggtg ggcacgctct
      121 tcgccgccgg agtcgtaccg ggcatcgtcg tcgccgtttt cctctgcgtc acggtgtggc
      181 tgatcagtag aaaacacggc tacaggggcg ctccgagggg tacttccaag gaagttcttt
      241 ttgcgctgaa ggacgctttc tgggggctct tcacaccggt gatcatcctc ggaggcattt
      301 acggaggggt cttcacgccc acggaggccg cggcggttgc ggtgttctac ggtctgttcg
      361 tcggcgtctt catctaccga agtctcacgc tccgcattct ctacgacata ctccgggaga
      421 cggtcgccgg gacttccgtg gtgatgatcg tggtcacctg cgcagggctc tactcctggc
      481 tcggcgccac cgtgggactc attgacaaga tcgcggcgct tcttctgggc atctccgaga
      541 atccgctggt cattctgctt atgatcaaca tcatcctgct cttcgcaggg atgctgcttg
      601 acgcgatctc gatcgcctac gtcttcctcc cgatcctggc gccggttatc gtcaccttcg
      661 gttgggatcc catctggttc ggcgtgatga tgaccgtcaa catggccata ggccaggtca
      721 ccccgccggt ggcggtgaac ctctatgtcg gcgcgagaat cagcggactc acgatggagc
      781 agatcacccg accggtcata ccgctcctcg tggcgtcgat cattgcgctt gccgtcatta
      841 ccgtattccc ggggatcacg ctctacctgc cccgacttct gggcatgatg tagcgcggac
      901 agcgtatctg cctgtcgttg tacagcgtat gttctttcgt tcctcaaaag gaggggtttt
      961 tgaatggctt cacccgtaac aatgcccaag ctcgggctga caatgaacac aggggttgtc
     1021 tcccagtgga agaagaagga gggagagccc gtccggaaag gcgagattct gatggtcgtc
     1081 gccacggata agctgacgtt cgacgtggaa tcgccctccg aaggcgttct gctgaaagtc
     1141 ctcgtccctg aaggaaagga tgttccgatc ggcgagattc tcgcctacat cggcgccgcc
     1201 ggcgaaacag ttgcgggcgc agctccggaa atgcccgcag cggcgcccgc ggcggaggag
     1261 aagaaggccg ccgctccttc cgaggttcgc agcgctccgg cgggcggaat ccgcgccacg
     1321 ccgcttgccc gtaagacggc gcgtgaggcg gggattgacc tctcgctcgt cgccggttcg
     1381 ggaccgtcgg gtcgtatcgt ccgaaaggat gtggacgccg cggccgcctc gggcggcggc
     1441 gctgtcaagg caagcccggt cgcggcgaaa atggctgcgg agctgggcgt cgacatggct
     1501 tccatgcgca aggatggtcg tgtgatgaag gaggacgttc ttcgtgccgc cgccgttccg
     1561 gaggcggagg atgtccgcat tccgctgacg ccgatgcgga aggtcatcgc cgagcggatg
     1621 agcctgagca actccacggt tccctcggtg acgtacacac tcgaagcgga cttctcggcg
     1681 ctcgttgcct tcagaaacaa ggtgaaggac gaagcggcga agaagggcgt caagatctcc
     1741 tacaatcata tcctgatgaa gatctgcgcc gcagcgctca aggagaatcc gcttgcgaac
     1801 gcctccttcg aaggggacgc catcgtgatg cac
//
LOCUS       DAOPKU010000602         1828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_75430_length_1828_cov_4.893401, whole genome shotgun sequence.
ACCESSION   DAOPKU010000602 DAOPKU010000000
VERSION     DAOPKU010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75430_length_1828_cov_4.893401"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PK849_14055"
     CDS             complement(<1..673)
                     /locus_tag="PK849_14055"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HPE67301.1"
                     /translation="MNFVALGIGGLMIAGGAYLSFIQKKKSEGLVTELKFMKAVSLGE
                     LKESWQSLSDEGMADGFRDMVETNGKAGTDGEMRAPYSNTPCAYFEATVTREFEKEER
                     STDKDGKVQTTRTRSSEVVSSRKSPSPLYIADGDIRVGIDMDGATLHLKDGEDRFEPY
                     ESNKTYTFFGVQFSAPSGVRTLGFRYKEKLIPLGHPLYVVGEARHSAGALRIGKPSEK
                     GKPFIV"
     gene            complement(707..1141)
                     /locus_tag="PK849_14060"
     CDS             complement(707..1141)
                     /locus_tag="PK849_14060"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPE67302.1"
                     /translation="MHKFPVLKDGVNGPMLEEVRAKVNTWLREFFDEDDLVRVEDLVS
                     FSFGSATIQVTVLPWHSGDVLVKVFSYLAENVDKNKVAAEFLRLNADLPLGGFSLVFD
                     NTVMLSCSLPGAKLDKSELLGAVQTVAAYADQYDDILKELYR"
     gene            complement(1134..1808)
                     /locus_tag="PK849_14065"
     CDS             complement(1134..1808)
                     /locus_tag="PK849_14065"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HPE67303.1"
                     /translation="MSIFARVSDIFKANVNEILDRMEDPEKMVKQMIIEMEEALVKAT
                     SALAKAMANERNLRKQQENALSQAKQWEEKAAMALKAGNADLAKQALSKKIIADGQAK
                     QYDAMVAQASSVTSQLRSQLDTLKTRLDEARMKQATLVARAQTAKTQKEFSTVMGSNV
                     GQGAFAKFEKMEKKIEGMEAEAQAFSELSGDAPADDPFKDMEKDMQLEAEMAKLMEKM
                     GGGAGA"
BASE COUNT          332 a          613 c          459 g          424 t
ORIGIN      
        1 agacgatgaa gggcttccct ttttctgaag gcttgccgat gcgcagcgct cccgcagaat
       61 ggcgcgcctc cccgacgacg tacagtgggt gtccaagagg aatgagcttc tccttgtagc
      121 ggaagccgag cgtccgaact cccgagggag cggagaactg caccccgaag aacgtatacg
      181 tcttgttgct ctcgtacggt tcgaaccggt cctctccgtc cttgagatga agcgtcgctc
      241 cgtccatgtc gatgccgacg cggatgtctc cgtcagcaat gtagagcgga gaagggctct
      301 ttctggagga aacgacttcg gaacttctgg tacgcgtggt ctgcaccttt ccgtccttgt
      361 cggtcgaacg ctcctccttc tcgaattcac gagtgaccgt cgcttcaaag taggcgcacg
      421 gagtgttgct gtacggcgca cgcatctctc cgtcagtccc ggccttgccg ttggtctcga
      481 ccatgtctcg gaatccgtcg gccattccct cgtcggagag cgactgccac gactccttca
      541 gctcgccgag cgaaacggcc ttcataaact tcagctcggt cacaagccct tcagacttct
      601 tcttctggat gaacgagaga tacgcgcccc ctgcgatcat cagaccgccg ataccaagag
      661 ccacgaaatt catgcaacct cctcctttcc tccccttcag gggaaactac ctgtacagct
      721 ccttcaggat atcgtcgtac tgatccgcgt aggcggcgac cgtctgcacc gcccccagca
      781 gttcgctctt gtccaacttc gctccgggca gagaacagga gagcatcacg gtgttgtcga
      841 aaacaagcga gaagccgccg agcggcaggt cggcattcag tcgcaggaat tctgccgcaa
      901 ccttgttctt gtcgacattc tctgccaggt aggaaaagac cttcaccaga acatccccgc
      961 tatgccacgg aagcaccgtc acctggatcg tcgcgctgcc aaaggagaag gagacgaggt
     1021 cctccacccg gacgagatcg tcctcgtcga agaactcccg cagccacgtg ttcaccttcg
     1081 cccggacctc ctccagcatt ggtccgttca cgccatcctt gagtacggga aacttatgca
     1141 cccgctccac cccccatctt ctccatcagt ttcgccatct ccgcctcaag ctgcatatcc
     1201 ttctccatgt ccttgaacgg atcgtccgcc ggagcgtcgc ctgagagttc cgagaacgcc
     1261 tgcgcctccg cctccatccc ctcgatcttc ttctccattt tctcgaactt cgcgaaggcg
     1321 ccctggccga cgttactccc catgaccgtg gaaaactcct tctgcgtctt cgccgtctga
     1381 gcgcgggcga ctaaagtcgc ctgcttcatc cttgcttcgt cgagccgcgt cttcagcgtg
     1441 tcgagctgtg aacgaagctg ggacgtgacg ctggaagcct gcgccaccat tgcgtcgtac
     1501 tgctttgcct gtccgtcggc aatgatcttc ttggagagcg cctgcttcgc aagatcggcg
     1561 ttccccgcct tgagggccat cgcagccttc tcctcccact gtttcgcctg agagagcgcg
     1621 ttctcctgct gcttgcggag gttgcgctcg ttggccatcg ccttcgccaa cgccgacgtg
     1681 gcctttacaa gcgcctcctc catctcgatg atcatctgtt tgaccatctt ctccggatct
     1741 tccatcctgt ccagtatttc gttgacgttc gccttgaaga tatccgatac ccgcgcgaat
     1801 atgctcataa tccccctcct tgtgtgtt
//
LOCUS       DAOPKU010000603         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_75562_length_1826_cov_4.757764, whole genome shotgun sequence.
ACCESSION   DAOPKU010000603 DAOPKU010000000
VERSION     DAOPKU010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75562_length_1826_cov_4.757764"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /gene="eno"
                     /locus_tag="PK849_14070"
     CDS             complement(<1..562)
                     /gene="eno"
                     /locus_tag="PK849_14070"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522203.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPE67304.1"
                     /translation="MSSIIGVHGREILDSRGNPTVEVEVWLDSGASGRAAVPSGASTG
                     TYEAVELRDGGPRYLGKGVLNAVNNVNEKIAPELMGFDADDQAEVDAALIELDGTPNK
                     GDLGANAVLGVSLAVARAAADDHDLPLWSWIGGLGPFSLPTPMMNVVNGGAHADNNVD
                     IQEFMLVPHGAETFPEALRMGVETYHT"
     gene            complement(685..963)
                     /locus_tag="PK849_14075"
     CDS             complement(685..963)
                     /locus_tag="PK849_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPE67305.1"
                     /translation="MRLDKFLKLARLVKRRTVAQEMIEVGAVRINGRQCKPSAEVRVG
                     DSVEIAYPSRFLSLEVLCADEAALKRMPPEEAWSLREERRVDPSERPW"
     gene            complement(1032..>1826)
                     /locus_tag="PK849_14080"
     CDS             complement(1032..>1826)
                     /locus_tag="PK849_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67306.1"
                     /translation="GRSLRFALLPEGKDPDDLLSLPDGKALFSKALEDARPLVLHHIH
                     LRREMLRTPEKRRKASEDIISGIAMLSPVDASPYLPAVEAALGVLHHEFIPMLEEQRR
                     KHSSQAREPVRDVPPDEETGEEQQPPDLVEAALCALLWRDCDLRRSADPVEIVSMLSD
                     ARLQTLASALLSASSAEDMEIYWHDIGDTFPMKAIAAGGVYCDELERAHDPYTILVDI
                     LSVRKHKREYDLLKTKLSRGTAANDELMRFQKLAMILKSGKGKGTL"
BASE COUNT          351 a          593 c          510 g          372 t
ORIGIN      
        1 gggtatggta cgtttcgacg cccatacgca gcgcctcggg gaaggtctcc gcgccgtggg
       61 gaaccagcat gaactcctga atatcgacgt tgttgtccgc gtgcgctccg ccgttgacca
      121 cgttcatcat cggcgtcggc agcgagaagg gcccgagacc tccgatccac gaccacaggg
      181 gaagatcgtg gtcgtccgcc gccgcgcggg ctaccgccag cgacacgccg agaacggcgt
      241 tggcgcccag gtcgcccttg ttgggcgtgc cgtcaagttc gatgagcgcc gcgtcgacct
      301 ccgcctggtc gtcggcgtcg aaccccatca gttcgggcgc tattttttca ttgacgttgt
      361 tgaccgcgtt cagcactccc ttgccgagat agcggggacc gccgtcgcga agctccaccg
      421 cctcgtacgt tcctgtggac gcccctgacg gaacagccgc tcttccggaa gcgccgctgt
      481 caagccacac ttcaacttca accgttggat tgccgcgaga atcgagtatc tcccggccgt
      541 gcactccgat aatactgctc atacacctca actccttttc aagtacgctc ttcgcccgaa
      601 gatgcttttg acgcaccgga cgattccaaa acgccacaca cccggcccgc tggggaggaa
      661 ctgttcagct tgccgggcgc agtatcacca cggtctttcc gatggatcca cacgccgctc
      721 ctcccgcaga gaccatgcct cttcaggcgg cattcgcttc aacgccgcct cgtcggcgca
      781 caggacctcc agcgagagaa aacgcgaggg gtacgctatc tcaacggaat cgcccacacg
      841 gacttccgcc gaaggcttgc actgtcttcc gttgattcga accgccccga cttcgatcat
      901 ctcctgagcg actgttctcc gtttgacgag ccttgcaagc ttcaaaaatt tatccaatcg
      961 catcgcttcg ttcctccaga cacaagacca cagggaagta tatcaaaaaa gccccctgaa
     1021 tttcaattct ttcacaaagt cccttttcct ttcccgctct ttaaaatcat ggccagcttc
     1081 tggaagcgca tcaattcatc gtttgcggct gttcccctgg agagcttcgt cttgagcaga
     1141 tcgtactccc gtttgtgttt ccgtacggag agaatgtcca ccagaatcgt gtagggatcg
     1201 tgagcccgct caagttcgtc gcagtagact cctccggcgg cgatcgcctt catcgggaac
     1261 gtatcgccga tgtcgtgcca gtagatctcc atgtcctcag cggaagacgc cgagagcagc
     1321 gcggacgcaa gcgtctgcag ccgcgcgtcg gagagcatcg acacaatttc cacagggtcg
     1381 gccgatcggc gaaggtcgca gtccctccaa aggagcgcgc acaacgcagc ctccacaagg
     1441 tcgggaggct gttgttcttc gccggtctct tcgtccggag ggacgtcgcg gacaggctcc
     1501 cgtgcctgcg aagagtgctt ccttcgctgc tcctccagca tcgggatgaa ttcatgatgc
     1561 agcacgccga gcgccgcttc gacagccgga agatacggcg acgcgtccac aggggagagc
     1621 atcgcgattc cggagataat gtcctccgac gcttttcggc gcttctcggg agtacggagc
     1681 atctccctcc ggaggtggat gtggtggagc accagcggac gggcgtcttc gagagctttg
     1741 gagaagagcg ccttgccatc cggaagcgag agcagatcgt ccggatcctt tccctcggga
     1801 agcagcgcga agcgcaggct tcgccc
//
LOCUS       DAOPKU010000604         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_75587_length_1826_cov_3.308865, whole genome shotgun sequence.
ACCESSION   DAOPKU010000604 DAOPKU010000000
VERSION     DAOPKU010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75587_length_1826_cov_3.308865"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PK849_14085"
     CDS             <1..610
                     /locus_tag="PK849_14085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE67307.1"
                     /translation="LARIQLRLDKIDPESLREKFAANIDEIQSIVEQGLELARSLHTS
                     EKECPLDIVAFIESIVDDIKAQGENVTLADTPGENDSSLLVMARPTCLKRSVENLLSN
                     AILYAGAARISLAKNNGDIVIDIEDDGPGIPADMLEKVFEPYYRLEYSRNRESGGTGL
                     GLAIARNMAALNNGSIRLINRREGGLLARISLPSLQEKTAVH"
     gene            890..>1826
                     /locus_tag="PK849_14090"
     CDS             890..>1826
                     /locus_tag="PK849_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPE67308.1"
                     /translation="MKKKEILRLLLSLCILAYSFTAAFAADNVRPEPLLIQAQGSFAV
                     GGTILAESGTFDPKNPMKPDGQTFHGDHAYVFHQVPVNPRAFPIVFLHGAGQFSKTWE
                     TTPDGREGFQNIFLRRGFATYLLDQPRRGGAGRSTLPMELKPAGDEQMWFSIFRLGNW
                     PNFHPGVQFPQDEESLNQFFRQMTPNTGPYDDRIISDAIVALFDKLGGGILVTHSQGG
                     GPGWHTVLKSTKVNAVISYEPGLFIFPEGEVPPPMPSSSPFGELQAAGVPMQEFMKFT
                     KIPIALYFGDNIPEAPSDDPGKDNWRTRLQMAKLWV"
BASE COUNT          433 a          503 c          507 g          383 t
ORIGIN      
        1 tttggctcgc atacagctcc ggctggataa gatcgacccc gaatcgctgc gcgagaaatt
       61 cgcggcgaat atcgatgaga tacagtccat tgtcgagcag ggcctggagc ttgcgagaag
      121 tctccatact tccgagaaag agtgtccgct cgacatcgtc gcctttatcg aaagtatcgt
      181 cgacgatatc aaggctcaag gggaaaatgt cacgcttgca gatacacccg gagagaacga
      241 ctcttccctg ttggtcatgg cgcgtccgac gtgcttgaaa cgcagcgtgg aaaacctctt
      301 gtcgaatgcg attctctacg cgggagccgc acgcatttct ctggcgaaga acaacggaga
      361 tatcgttatc gacatcgagg acgacggtcc cggcattccc gccgatatgc tcgaaaaagt
      421 attcgaaccg tactacagac tggaatactc gcgcaacagg gagtccggag ggaccggtct
      481 cgggcttgcg atcgcccgca acatggcggc tttaaacaac ggttccatcc gtctgatcaa
      541 caggcgggaa ggaggtttgc tggctcgcat ttctcttccg tctctgcagg aaaaaaccgc
      601 tgtgcattga atactacaac tccggagcaa gctttcggca ttgatttcga aaggccgcgc
      661 cgaatggaat gcgagggatg attttctccg gttgccacga caggcaggtt gcgaaaaaaa
      721 ccaaccgccg gcgtcgctca accgaagacg atgaagagca tcgcggcgcc ggacaagaaa
      781 accggtgaac gctcaatcct ttgcacgcac gaaaacgccg attccacttg actctgttat
      841 tcaatataga gagaaaaaga gtatattcca tataaggagg accattgata tgaaaaagaa
      901 agagattttg aggctcttgc tatcgttgtg cattttggcg tactctttca cagccgcttt
      961 cgctgccgac aacgtcagac cagaaccgct tcttattcaa gcgcagggga gctttgcagt
     1021 cggaggcacg atccttgcgg agtccggaac gttcgatccg aagaacccca tgaagcccga
     1081 cgggcagaca ttccacgggg atcatgccta tgttttccat caggttccgg tcaacccgcg
     1141 cgcgtttccc atcgtctttc ttcacggagc ggggcagttc tcgaaaacct gggaaacgac
     1201 gccggacggg cgagaggggt ttcaaaacat cttcctgcgg cgcggattcg ccacctacct
     1261 tctggatcag cctcgccgcg gaggcgcggg gagaagcacg ctgcctatgg agctgaaacc
     1321 cgccggagac gagcagatgt ggttttcgat attccgcctc gggaactggc cgaactttca
     1381 ccctggtgtt caatttcctc aagacgagga aagcctgaac cagttcttcc gtcaaatgac
     1441 gccgaatacg gggccgtacg acgaccggat tatttccgat gcgatcgtcg ctctcttcga
     1501 caaattgggc ggaggcatcc tggtcacaca ttctcaggga ggcggtccgg gatggcacac
     1561 cgtactgaag agcacgaagg tcaacgccgt catctcctac gaacccggtt tgttcatctt
     1621 ccccgaaggc gaagttcctc cgccgatgcc gagttccagt ccgttcggcg agttgcaagc
     1681 ggcaggcgtg ccgatgcagg agttcatgaa gttcacgaag atcccgatcg cgctctactt
     1741 cggagacaac attcccgagg cgccttcgga cgaccctgga aaggacaact ggcggactcg
     1801 ccttcagatg gcgaagcttt gggttg
//
LOCUS       DAOPKU010000605         1815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_76263_length_1815_cov_4.651136, whole genome shotgun sequence.
ACCESSION   DAOPKU010000605 DAOPKU010000000
VERSION     DAOPKU010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76263_length_1815_cov_4.651136"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PK849_14095"
     CDS             <1..507
                     /locus_tag="PK849_14095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPE67309.1"
                     /translation="PTEKVTFEMWKFVVDLNLMGVVYGTYSAIPVMREQGFGHIVNTG
                     SITGRVPVPYQAVYAATKAAVISMTESLQFELETEGLNFSVFCPGNVATAIFGDVPPP
                     PDAIGVDDAVEYILAEVEKKSLVIILPQQMRDFDAVYREHREEYDAFARKMAAERREN
                     YRTKGTYF"
     gene            544..1779
                     /locus_tag="PK849_14100"
     CDS             544..1779
                     /locus_tag="PK849_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67310.1"
                     /translation="MRRGLFDEACLPLETVLLFIAGMTVFIAGILLFPVYAGVAPYYE
                     NGLYGLLLFFFALQTVMLGKTPFGELPVSKPLLAVGVTIASIGIVTCFIPGIPNKLAR
                     WMLVLCLGPGGALLLLQMLFSKNKLRLWLRVKGALRPLPFACGAVYALSMLAGLLVLK
                     PELLTGYGTATAVLLYGAAILNLSRVLAVVYQEYPEASADAGDKGGLPFDKAMLMLTG
                     VFMVLLGGLLVPVNLGLLPFSGSAQLGLLMVIFSVQMLAAGSTPIGVFPRSWLMAYLG
                     TLFAGLGIVSCIVPDILVAPLTLLVGVLNILSGGLTLAKALPSLLKTRKMMPSVPVPS
                     LLLRLYSTQIALGGLSVMFGSSMLAPGIIPGLLIGVILAANGGVLLYLMRLLFQLDAL
                     AESAEDGGSAGGDAQNECS"
BASE COUNT          289 a          577 c          573 g          376 t
ORIGIN      
        1 cccacggaga aggtcacctt cgagatgtgg aaattcgtgg tcgacctcaa cctgatgggc
       61 gtcgtctacg gcacgtacag cgcgatcccc gtcatgcgcg agcagggctt cggacatatc
      121 gtcaacaccg gctcgatcac ggggcgggtt cccgttccgt accaggccgt ctacgccgcc
      181 acgaaagccg ccgtgatctc catgacggag agcttgcagt tcgagctgga gacggagggg
      241 ctcaacttca gcgtcttctg cccgggcaac gtcgccaccg cgatcttcgg cgacgttccg
      301 ccgcctcccg acgctatcgg cgtggacgac gcggtggagt acatcctcgc ggaggtggag
      361 aagaaatcgc tcgtgatcat cctcccgcag cagatgcgcg attttgacgc ggtctataga
      421 gaacacaggg aggagtacga cgccttcgcc cggaagatgg ccgccgagcg ccgggagaac
      481 tacaggacaa agggaacgta cttttaggcg cgtgcgccga cgaaggaaag gaggcgcgat
      541 ccgatgcgcc gcggactgtt tgacgaagcg tgtcttccgc tggagaccgt gttgctgttc
      601 atcgccggga tgacggtgtt catcgccgga atcctgctct tccccgtgta cgcgggcgtg
      661 gcgccgtact atgagaacgg gctgtacggc cttctgctct tcttcttcgc cctgcagacc
      721 gtcatgctcg ggaagacgcc gttcggcgag cttcccgtgt cgaaaccgct gctcgcggtc
      781 ggcgtgacga tcgcctccat cgggatcgtg acctgcttca tccccggcat cccgaacaag
      841 ctcgcccgct ggatgcttgt tctctgcctc ggccccgggg gagcgctcct gctgctccag
      901 atgctcttct ccaaaaacaa actccgtttg tggctgcgcg tcaagggcgc gctgcggccg
      961 ctgcccttcg cgtgcggagc ggtctacgcg ctgtccatgc tcgccgggct gctcgtgctc
     1021 aagccggagc tgctgaccgg atacggcacg gcgacggcgg tgctcctcta cggcgcggcg
     1081 atactgaatc tctcgcgcgt tcttgccgtc gtctaccaag agtaccccga ggcctctgcg
     1141 gatgcgggag acaagggcgg cctccccttc gacaaggcga tgctcatgtt gaccggagtt
     1201 ttcatggtgc tgctcggagg cttgctggta ccagtgaacc tcggcctgct tcccttctcg
     1261 gggagcgcgc agctcggtct gctgatggtc atcttctcgg tgcagatgct cgcggcgggg
     1321 agtacgccga tcggcgtatt ccctcgttca tggctgatgg cgtacctcgg gacgctcttc
     1381 gcagggctcg gcatcgtctc ctgtatcgtt ccggacatcc ttgtcgcgcc gctgacgctc
     1441 ctcgtcggcg tgctcaacat tctgagcggg ggcctcacgt tggcgaaggc gctcccctcg
     1501 ctgctgaaga cgagaaagat gatgccgtcc gttccggttc cttcgctcct cctcaggctg
     1561 tactcgacgc agatcgccct ggggggactc tcggttatgt tcggctcgtc gatgctcgcg
     1621 cccggtatca tccccggcct gctcatcggc gttattctcg ccgcgaacgg cggcgtgctg
     1681 ctctacctga tgcgtctgct cttccagctc gacgccctcg cggaatccgc cgaggatggc
     1741 gggtcggcgg gcggcgatgc tcaaaacgag tgttcgtaac tacggaaaaa aaggaggttt
     1801 tcgaaatgaa aagcg
//
LOCUS       DAOPKU010000606         1813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_76401_length_1813_cov_4.668942, whole genome shotgun sequence.
ACCESSION   DAOPKU010000606 DAOPKU010000000
VERSION     DAOPKU010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76401_length_1813_cov_4.668942"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PK849_14105"
     CDS             <1..1206
                     /locus_tag="PK849_14105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE67311.1"
                     /translation="GNSFFSAPISEAQYLALVMERNNDLAAISQEIEARLFSARAELS
                     VQRPSLGLSAEASKWLDQERGDQQIDLSLSHRVNLSGTYGLQERDLLLGVEILRNQYA
                     DAVNERLALAASAYRQAVMASLNREAMEQIVAKRRESMLITREKFDKELVSLLELLRA
                     QSQVDDGEAFLLQARQKYEQQLVEMSALAGGVSVTPEIMLPQLEELRPDIDEKKAWGL
                     RPDVASLMLSRERASVQRSLAAKGLSPFVDLSVGLRVRQDSSSAIADERAGEVFVRAV
                     LTVPLTDGGKTANTTEAAALLLRKAEYELEARRDSLKKEAELVRERWGRALEIVTIRR
                     RQAERADKELEIAEMMYREGLGSQLDLITAQEADQKSRTELINAIQELWLVLAEADRV
                     MGRWVRRGE"
     gene            complement(1367..1675)
                     /locus_tag="PK849_14110"
     CDS             complement(1367..1675)
                     /locus_tag="PK849_14110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HPE67312.1"
                     /translation="MSMESAAAFYERLQSDAALQENLKAAGNRDQVEAYVKGELGYDF
                     TKEEMQKVVFERNPEMTDEELEAVIGGASNDMMWGITIGVGIGGGLGIIALAIAAAGA
                     "
BASE COUNT          347 a          593 c          520 g          353 t
ORIGIN      
        1 gggaattcct tcttctccgc gccgatttcg gaagcacagt acctggcgct tgtgatggag
       61 cgaaacaacg accttgctgc gatcagccag gagatcgagg cgcggctttt ctccgccagg
      121 gcggagcttt ccgttcagcg cccctctctt gggctctccg ccgaggcaag caagtggctc
      181 gaccaggaac ggggcgatca gcagatcgat ctttccctct cccaccgcgt caacctctcg
      241 ggaacctacg gactccagga acgggacctt ctcctcggag tcgagattct ccggaaccag
      301 tacgccgatg cggtcaacga acggctcgcc ctcgccgcgt ccgcctaccg ccaggcggtg
      361 atggcttcat tgaaccggga ggcgatggag cagatcgtgg cgaagcgccg cgagtcgatg
      421 ctgatcaccc gcgagaagtt cgacaaggag ctggtctccc tgctggagct gcttcgggct
      481 cagtcgcagg ttgatgacgg cgaagcgttt ctcctccagg cacgccagaa gtacgagcag
      541 cagctggtcg agatgagcgc cctggcaggg ggagtttcgg tcacgccgga gatcatgctc
      601 ccccaactcg aggaactgcg tcccgacatc gacgagaaga aggcatgggg gctccgtccg
      661 gacgtcgcct cgcttatgct ctccagggaa cgcgcctccg tgcagcgctc gctcgccgcg
      721 aaggggctct ctccgttcgt tgacctctcc gtcgggctgc gggtgagaca ggactcctcc
      781 tctgccatcg ccgatgagag ggccggagag gttttcgtcc gcgccgtgct gacagttccc
      841 ctgacagacg gggggaaaac ggccaacaca accgaggcgg ccgcgctgct tctccgaaag
      901 gccgagtacg aactggaggc gaggcgcgac agcctgaaga aggaggcgga gctcgtccgc
      961 gaacgatggg gacgggcgct cgaaatcgtc accatccgcc gccgccaggc cgagcgggcc
     1021 gacaaggagc tggagatcgc cgagatgatg taccgcgagg ggctgggatc ccagcttgac
     1081 ctgatcacag cgcaggaagc cgatcagaaa agcaggacgg agctgataaa cgcaatccag
     1141 gaactgtggc tcgtcctcgc cgaagccgac agggtgatgg ggcggtgggt ccggcgcggg
     1201 gagtagtacc cgaccccatg gaggccgaag aagaccagtc ctatgaattc gaacaaccgg
     1261 cctccacctg cggcagcttc ctgctcagcg tcggtcatct cccacaattc catctcttcc
     1321 cgacggaacg gacagttcct gaaggtttcc tctctctctt ttttcgctac gcacccgcag
     1381 cggcgatcgc aagggcgata atccccagcc caccgccgat gccgacgccg atggtgatcc
     1441 cccacatcat atcgttgctt gctcctccga tgacagcctc cagctcttcg tcggtcatct
     1501 ccggattccg ttcgaaaacc accttctgca tctcttcctt tgtgaagtca taaccaagtt
     1561 cccccttcac gtacgcttcc acctgatctc tgttccccgc cgccttcagg ttttcctgaa
     1621 gcgccgcgtc gctctggaga cgctcataga acgccgctgc agattccatc gacatataat
     1681 acgacctcct tttatactct ctcccttttc ggaagaggtt atatacacac ctgcaatttc
     1741 tgcggcagcc tccagttcct cgtcggtcat ctccggattc ttcccgaaga tcactttctg
     1801 catctcctcc agc
//
LOCUS       DAOPKU010000607         1810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_76606_length_1810_cov_4.785185, whole genome shotgun sequence.
ACCESSION   DAOPKU010000607 DAOPKU010000000
VERSION     DAOPKU010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76606_length_1810_cov_4.785185"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="PK849_14115"
     CDS             <1..1619
                     /locus_tag="PK849_14115"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE67313.1"
                     /translation="LFLAAALLAGGYFLYGTDGNATWYVAGLLLLFILMFFKSIFSCS
                     GPAAVVSEWIGRLSRNESVDLRKSVDVPQGSKLSSVANALNAFVQVMRTHFLRIMQGL
                     HKFTFNFYRLERQLGEFFSAFSAMSAGVKEGISSGQNVSHATESQYASSEEISATAQG
                     LAQLAAELNNAVASAGDRADAGNRRLREMERTFASVVEETKTLSEEARILSGKVDVIQ
                     SVVHTITGIAEQTNLLALNASIEAARAGDAGRGFAVVADEVRKLAEESKTAAATISKN
                     LQELVSGVHTTSGGVETMAAKMNEANENVGAVLSEISALLEGISGISDSSERVAASAQ
                     ELGASSEELAASAETVTRETEKMSSIFGDIEGKISSLSSTAEGLNETSKEGSIDAAAL
                     IHQLSVLKAMKADDFADIAEDAIKAHKGWVANLKKFVEGGQWDLETNPQRCRFGIFLS
                     FIERPEGASEELWSGILSMHEKLHGLGHTVNDAMQRGESGKAREVLKETVALSERLSA
                     SLLRVVEICRGQGEQEREASGLPALPERTR"
BASE COUNT          351 a          550 c          593 g          316 t
ORIGIN      
        1 tcctctttct tgcggcggcg ctccttgcgg gcggttattt cctgtacggg acggacggaa
       61 acgcgacctg gtacgtggcg ggactcctcc tgcttttcat tcttatgttt ttcaagagca
      121 tcttcagctg ctccggccct gccgctgtcg tctccgagtg gatcgggagg ctttcgcgga
      181 acgagtcggt cgatctgcgc aagagcgtcg acgtgccaca gggttcgaaa ctgagctccg
      241 tggcgaacgc cctcaatgcg ttcgttcagg tcatgcggac gcactttctg cgcatcatgc
      301 aggggctgca caagttcaca ttcaacttct accgtctgga gcggcagctc ggcgagttct
      361 tttcggcctt ctccgcgatg tccgcaggcg tgaaggaggg gatctcctcc gggcagaacg
      421 tctcgcatgc gacggagtca cagtacgcct cgtccgagga gatttcggcc accgcgcagg
      481 ggctggcgca gctggcggca gaactgaaca acgccgtcgc ctccgcgggc gacagggcgg
      541 acgccgggaa cagacgcctc cgcgagatgg agcgcacctt cgcctccgtc gtcgaggaaa
      601 cgaagacgct ttcagaggaa gcgcgcatcc tctccggcaa ggtggacgtc atccaaagcg
      661 tggtgcacac gattacggga atagcggagc agacgaatct cctcgcgctc aacgcttcga
      721 tcgaggcggc ccgcgccggg gatgccggcc gggggttcgc cgtggtcgcc gacgaggtgc
      781 gcaagctggc cgaggagagc aagacggccg ccgcgacaat ctccaagaac ttgcaggagc
      841 tcgtctccgg agtgcacacc acctcgggag gcgtcgagac gatggctgcg aagatgaacg
      901 aagccaacga gaacgtcggc gccgtcctct cggagatttc cgcgctgctc gaaggtattt
      961 cggggatttc cgactcgtcg gagcgcgtcg ctgcaagcgc gcaggagctt ggcgcgtcct
     1021 ccgaggagct ggcggcctcc gcggagaccg tcacccgcga gacggagaag atgagctcca
     1081 ttttcggaga catcgagggg aagatatcct ctctctcttc caccgccgaa gggctgaacg
     1141 aaacttcaaa ggagggctcg atcgacgccg ccgccctcat ccaccagctt tccgtcctca
     1201 aggcgatgaa ggcggacgac ttcgccgaca tcgcggagga cgcgatcaag gcgcataagg
     1261 gctgggtggc caatctgaag aagttcgtgg agggcggaca gtgggacttg gagacgaatc
     1321 cgcagcgctg ccgcttcggc atcttcctct ccttcatcga gcggcctgag ggagcttccg
     1381 aagagctgtg gagcggcatc ctctcgatgc acgagaagct gcacggtctg gggcacacag
     1441 tcaacgacgc gatgcagcgc ggcgagagcg gcaaggcgcg ggaggtcctg aaggagaccg
     1501 tagccctcag cgaacgtctt tcggcatccc tgctccgcgt ggtcgagatc tgccgcgggc
     1561 agggggaaca ggagcgggaa gcctcggggc tcccggcgct tccggaaaga acgcggtaag
     1621 cggcaaggac aattctataa aagaagaggg ccgaagcccc ccgcaagggg atgcctcggc
     1681 cctctttttc gtctttaatc agcagctaac agctgccgga acaaccggct cacccgccgc
     1741 cgcagctgcc gctccggagg cgagatattc cagcaagagc tggatttccg ccgcatactg
     1801 gtagtgcaat
//
LOCUS       DAOPKU010000608         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_77205_length_1801_cov_2.147194, whole genome shotgun sequence.
ACCESSION   DAOPKU010000608 DAOPKU010000000
VERSION     DAOPKU010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77205_length_1801_cov_2.147194"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PK849_14120"
     CDS             <1..834
                     /locus_tag="PK849_14120"
                     /inference="COORDINATES: protein motif:HMM:NF037272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HPE67314.1"
                     /translation="HAVNIVGWDDEYPAGNFNTDPGRDGAWIMQNSWGTEWGEKGFFY
                     LSYADPTIGTPSIYIGGETTEFERIYQYDPLGWVESFGFGSETAWFANIFTSAPSVSH
                     DNAGESTVELLRAVSFYAGQANAPYTIEIRRGVDENAPTSGALVNSTSGTLAAAGYHT
                     VNLSSSVPLSPVERFSVVVRLTTPGYLFPVPVEMPSSGYSEKATAGAGQSFVSFDGAS
                     WSDMTTRSPNTNVCLKAFSSTGEPPSSSSGGGCIVPHGAHIYSLLLLLPLLALLGKNA
                     R"
     gene            complement(1263..>1801)
                     /locus_tag="PK849_14125"
     CDS             complement(1263..>1801)
                     /locus_tag="PK849_14125"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPE67315.1"
                     /translation="LPPKAVFSPVMMRYLERVAEVTPPFRVAVEVRNRSWMEPGNRES
                     FLDLLKGANMAYVAVDEPELPWTVGRDAPVTASWGSVVRFHGRNTEAWSRKDATVAER
                     FRYCYSDAEMLSWEGAVRDAATKAERLFLMFNNCFRDYAVQNAQRMKCLLGVACAPGE
                     GVQGELDFEGSADESKIE"
BASE COUNT          376 a          548 c          452 g          425 t
ORIGIN      
        1 cacgccgtca acatcgttgg gtgggacgac gagtatccgg caggtaattt caacacagat
       61 ccggggcgcg acggcgcatg gataatgcag aacagctggg gaaccgaatg gggcgaaaag
      121 ggctttttct acctctccta cgccgatcct actattggca ctccgtcgat ctatatcggc
      181 ggagaaacaa cggaattcga gcgcatctat caatatgatc cgctcgggtg ggtggagagc
      241 ttcggtttcg gctccgaaac agcatggttc gcgaacatct tcacctcagc cccttccgtc
      301 tctcatgata atgcaggcga gagcacagtc gaacttcttc gcgcagtctc cttctacgcc
      361 gggcaggcca acgccccgta cacgatcgaa atccgacgcg gagtcgacga gaacgccccg
      421 acgagcggcg cccttgtgaa ttctacttca ggcacactcg cggcagccgg atatcatacc
      481 gtgaatcttt catcgtccgt tcctctctct cccgttgaaa gattctccgt ggttgtccgc
      541 ctgacaaccc ctggctacct cttccctgtt ccggtcgaga tgccttcatc cgggtacagc
      601 gaaaaagcga cggctggcgc cggacagagt ttcgtcagct tcgacggggc ttcctggtcg
      661 gacatgacaa cgcgctcccc gaatacgaac gtctgcctca aggcgttcag ctcgaccgga
      721 gaaccgccct cttcctcttc cggcgggggg tgtatcgtac ctcatggggc gcacatatac
      781 tctctcctgc tccttttacc tttacttgcg ctcttaggaa aaaacgcacg ctaaaaaaat
      841 gcggatgtga tgtgaaaggg cgctccacgt tgctccttcc acatcacatc cgcacacaac
      901 gaaaagggag gggaataact tcccctcccg gaatttcaac gggctatctc ggacggtggt
      961 tctgaccttc tgtcaaacaa ccagctccta gtacttctgt agatatccca cacgtatctt
     1021 tgttgaggtt ctgtacagct ttccttgttg cggcagttct ttggttcgtg gtcctcgttt
     1081 cagttgctgt ctttggtgat cggctttcct gacttgaaac tctttgatgc ttcttctctc
     1141 cgtccggata acccgttagg agtattctac acttgcacgc tgcatccgtc aaacagacag
     1201 agtatacagt ctcggggata taatccttat tttaagccgg gaatgctgtt tttcttcatt
     1261 tttcattcaa ttttactctc atccgcggag ccttcaaaat ccaactcccc ctgcactcct
     1321 tctccaggag cgcaggcgac cccgagcaga catttcattc gctgcgcatt ctgcaccgcg
     1381 tagtctctga agcagttgtt gaacatcagg aagaggcgct ccgcttttgt cgcggcgtca
     1441 cggaccgcgc cctcccagga gagcatctcc gcatccgaat aacaataccg gaatcgctcc
     1501 gcgacggttg catctttgcg gctccatgct tccgtattgc gtccgtggaa gcgaacaacc
     1561 gacccccacg aagcggtgac gggggcgtcc cttcccacgg tccagggcaa ttcaggttcg
     1621 tccaccgcga cgtacgccat gttcgcccct ttcaacaaat ccaggaacga ctcccggttg
     1681 cccggttcca tccaggaacg gttccgaacc tcgaccgcga cgcggaaggg cggggtcacc
     1741 tcggcaacgc gttcgagata gcgcatcatc acgggggaaa aaacagcctt gggaggaagt
     1801 t
//
LOCUS       DAOPKU010000609         1800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_77221_length_1800_cov_5.182235, whole genome shotgun sequence.
ACCESSION   DAOPKU010000609 DAOPKU010000000
VERSION     DAOPKU010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77221_length_1800_cov_5.182235"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PK849_14130"
     CDS             <1..584
                     /locus_tag="PK849_14130"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPE67316.1"
                     /translation="RSLVWANSYQQQTGLAKIARQHLKELLRDVGETVHLFIYEKGEA
                     FYLEKMHTYNVTGLDSRIGSRLELYSTSAGRAILAALPEEEFEAYLSSRELLPRTDWT
                     VVDKETFGKIIEKARTEGYAEETQENEVGVRCVGAAILGKNGYPLGAVSVAGPVFRFT
                     DDRVESVGKRVFGTARIISYQLGYSTNGGQGGL"
     gene            605..1438
                     /gene="kduI"
                     /locus_tag="PK849_14135"
     CDS             605..1438
                     /gene="kduI"
                     /locus_tag="PK849_14135"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HPE67317.1"
                     /translation="MKLDIRHGANPCDFKSYDTERLRKDFLVTHLFVPGEIRMTYSHI
                     DRIITGGACPTIPLSLECGKEIGTEFFLQRREMGIINVGAPGSVTVDGERVSLKKMDG
                     LYLGAGGKNIVFESDKHDDHAHFYFSSTPAHTSYPNCYIDMEKAKKVKMGEPEKANVR
                     TINQYIHPEVCTTCQLMMGLTQLESGSVWNTMPCHTHERRMEVYFYYDIPQDGVTFHI
                     FGQPEETRHIVVRNEEAVINPSWSIHSAAGTSNYSFIWSMAGENQVFTDMDHVPMSAM
                     K"
     gene            1602..>1800
                     /locus_tag="PK849_14140"
     CDS             1602..>1800
                     /locus_tag="PK849_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67318.1"
                     /translation="MDWGRVPADTVVIESKEITLRDVVQAAADGVDTPEGLMEVLGLE
                     EGQEGTEHLQPILDVFLPAIER"
BASE COUNT          437 a          512 c          537 g          314 t
ORIGIN      
        1 cgagaagcct tgtctgggcc aactcctacc agcagcagac ggggctggcc aagatcgcgc
       61 gccagcatct gaaggagctg cttcgggacg tcggagagac tgtacacctc ttcatctatg
      121 aaaagggcga ggcgttctat ctggaaaaga tgcataccta caacgtcacg gggcttgatt
      181 cgcgaatcgg atcgaggctt gaactgtaca gcacctccgc ggggagggcc atactggccg
      241 cgcttccaga ggaggagttc gaggcgtacc tctcttccag agaactgctt ccgcgcaccg
      301 actggaccgt cgtcgacaag gagacgttcg ggaagatcat cgagaaggcg cgaacagaag
      361 ggtatgccga ggagacccag gagaacgagg tcggcgtccg ctgcgtcggc gcggcaatcc
      421 tcgggaaaaa cggctaccct ttgggggcgg tcagcgtcgc cggccccgtc ttccgtttta
      481 ccgacgacag ggtggagagc gtgggcaagc gcgtgttcgg cactgcgcgg attatatcct
      541 accagcttgg ttacagtaca aacggaggcc agggcggtct ctaaaaaata aggagggact
      601 catcatgaaa ctcgacatcc ggcacggagc gaatccgtgc gacttcaaga gctacgacac
      661 cgaacggctg cggaaggatt ttctggttac ccatcttttc gtccccggag agatcagaat
      721 gacgtacagc catatcgacc gcatcatcac cggcggcgcc tgcccgacca ttcctctgtc
      781 gctggagtgc ggcaaggaga tcgggacgga gtttttcctc cagcggcgcg agatggggat
      841 catcaacgta ggggctcccg gctcggtcac cgtcgacgga gagcgcgtct ccctcaagaa
      901 gatggacggg ctctacctcg gagcgggcgg caagaacatt gtcttcgaga gcgacaaaca
      961 cgacgatcac gcgcacttct acttcagcag cacccctgcc catacttcgt accccaactg
     1021 ctacatcgac atggaaaaag cgaaaaaggt caaaatgggc gagccggaaa aggccaacgt
     1081 ccgcactatc aaccagtaca tccacccgga ggtatgcacc acctgccagc tcatgatggg
     1141 gctcacgcag ctcgaaagcg gcagcgtctg gaacaccatg ccctgccata cccatgagcg
     1201 gcgcatggag gtgtacttct actacgacat tccccaggac ggggtcacat tccatatctt
     1261 cggccagccc gaggagaccc gccacatcgt cgtccgcaac gaggaggccg tcatcaatcc
     1321 gagctggtcc atccacagcg cggcgggcac gtcaaactac agcttcatct ggagcatggc
     1381 gggagaaaac caggtgttca ccgacatgga ccacgttccg atgtcggcga tgaaatagga
     1441 aaatgcaagg ggctccattc gaagaaaata ccctctagcg gcatatacga aaaaacacct
     1501 atggcgcatt tgtcgaaaga aggtataata tcgaaagctt tttcttccgg agcgccatgt
     1561 tcggaaaaag ggctgacttt actgaggaag gaggagtcgt catggattgg ggaagagtgc
     1621 ccgcggatac cgtagtgatc gagagcaagg agatcacgct gcgcgacgtg gtgcaggcgg
     1681 cagccgacgg cgtcgacacg cccgagggac tgatggaagt cctcggcctt gaagaggggc
     1741 aagagggaac cgaacacctg cagcccattc tggacgtctt tcttccggcc atcgaacgcc
//
LOCUS       DAOPKU010000610         1799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_77284_length_1799_cov_4.237959, whole genome shotgun sequence.
ACCESSION   DAOPKU010000610 DAOPKU010000000
VERSION     DAOPKU010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77284_length_1799_cov_4.237959"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..1231)
                     /locus_tag="PK849_14145"
     CDS             complement(410..1231)
                     /locus_tag="PK849_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HPE67319.1"
                     /translation="MKTPAELAKLACTVSKVKGNLSVRQMVLMGILAGAYIAFAGWLM
                     TVVSHDMTQHFGSGMTRLVSGAVFSVGLMMVVISGSELFTGNCMMPLGYLAGCTPLPK
                     ILRNWGWVYVSNLVGSILIAALIYYSGLADNAVGGRALQVASGKMGLSFGQAFFRGVL
                     CNWIVVLAVWMAMAATDIVGKVWAIFFPIMTFVAAGFEHSIANMYFMALGIFLKGTPA
                     AVTASGLSEQALANVGLGGYMSNLIPVTLGNMVGGILFVAVFYYFIFKDGLTDID"
     gene            complement(1323..>1799)
                     /locus_tag="PK849_14150"
     CDS             complement(1323..>1799)
                     /locus_tag="PK849_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HPE67320.1"
                     /translation="LPWPCPTCEHPGTPILYTTGFPRGKADFSPCDWVKPDEWPTEEY
                     PFIATTGRILYHYHSGSMTRRSSTGEFIKEMYIEVNPADATALNVKDGEQIKVTSRRG
                     EVSGSVKITDRVPAGTVFLPFHFSETSANILTSAKIDPYSKTPGFKVNAVKLEKTV"
BASE COUNT          421 a          496 c          479 g          403 t
ORIGIN      
        1 tcgtttcagc aagcacacca tcccagtcaa gcacaaatgc acggcataat gcgggattcc
       61 aatggtgtga gcggcgttgt tggagcactg gatacccctc ctcatataaa agacaggggc
      121 gcgggcttcc ccgtcccctg tagccaagct tccccgcaat gtcgtgtcat gaacgtcttg
      181 acccgttcct atagtattgg ggcctgctga accttagttt cgtcatcgca cgaggcaatt
      241 cctctaccgg cccaaactgg cggccccgca aggcgcgagt gttggctcaa gacaaaaatc
      301 atgatcacgg gcaccgcagg gactctttct ttcttgttgt atattatagc acaaaaatgg
      361 gaatcgccca gtcgcaggcg attcccaaaa gagagaaaag ataaaatgac taatcgatgt
      421 ctgtgagacc gtccttgaag atgaaatagt agaacacggc gacaaagaga attccgccga
      481 ccatattccc cagcgtaacg ggaataaggt tgctcatgta gccgcccagg ccgacgttgg
      541 caagcgcctg ctcggaaagg ccggatgcgg ttacggctgc cggcgttccc ttcaggaaga
      601 tcccgagagc cataaagtac atgttcgcga tggagtgttc aaaaccggcc gcgacgaatg
      661 tcatgatcgg gaagaagatt gcccatactt tcccgacgat atctgtcgcc gccatcgcca
      721 tccagacggc gagcacaacg atccagttgc agaggacgcc gcggaagaat gcctgtccga
      781 aggagagtcc catcttaccg gaagctacct ggagcgccct tccgccgacc gcattgtcag
      841 ccagcccgga gtaatagata agcgcagcga taaggatgct gccgacaagg ttcgaaacgt
      901 agacccaacc ccagttccga agaatcttcg gcagaggcgt acagcccgca aggtatccca
      961 gaggcatcat gcagtttccc gtaaagagct cggagccgct gataacaacc atcatgagtc
     1021 ccacgctgaa gacggctccc gaaacaaggc gtgtcattcc gcttccgaag tgttgcgtca
     1081 tgtcgtgaga cacgacggtc ataagccatc cggcaaatgc tatatacgct ccggcgagta
     1141 ttcccataag gaccatctgc cgcaccgaga gatttccctt tacttttgaa acagtacatg
     1201 ccaacttggc cagttctgca ggggttttca tcgttctctt cctcctgtga tagttttctt
     1261 attagatgta ttcagagctg cgatcggacc aactgctgcg gtccggtcgc agctctcttt
     1321 atttatacgg ttttctcaag ttttactgcg ttaaccttga aaccgggcgt cttggagtac
     1381 ggatcgatct ttgccgacgt aaggatattc gctgatgtct cgctgaagtg gaacggaagg
     1441 aagacggtcc cagcgggcac gcgatcggta atcttcactg agccgctgac ttctcctctg
     1501 cgggaagtaa ccttgatctg ttctccatcc ttgacgttca gcgcagtggc gtcggccggg
     1561 ttcacctcga tgtacatctc cttgataaac tcgccggtcg agctccggcg ggtcatgctt
     1621 ccggagtggt agtggtagag gatcctgccg gtagttgcaa tgaacggata ctcttctgtc
     1681 ggccattcat caggttttac ccaatcgcac ggagagaagt cagccttgcc tctggggaaa
     1741 cccgttgtgt acagaatcgg cgtcccgggg tgttcgcacg tcgggcatgg ccacggaag
//
LOCUS       DAOPKU010000611         1798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_77357_length_1798_cov_4.531842, whole genome shotgun sequence.
ACCESSION   DAOPKU010000611 DAOPKU010000000
VERSION     DAOPKU010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77357_length_1798_cov_4.531842"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PK849_14155"
     CDS             <1..1204
                     /locus_tag="PK849_14155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM/SPASM family putative metalloenzyme
                     maturase"
                     /protein_id="HPE67321.1"
                     /translation="SGPGIPDEHMTMSTFQRLVPAFPMLETLLLNGIGEPLLHPRLEA
                     FVQLAKEHLQEKARVGFQTNGTLLTPERARSLLAAGTNIVCLSVDATDPALFQSMREG
                     GETEDVNRAAAALNAARARASSPDFHWGAEIVLTRDTLDELPRVVEWVGERGGAFLLV
                     THLLPYRQESVPSVAYNPNVDETLAFYREKSREANEQGIDLSRYIEARWRFRPVDNGE
                     RTVAFMEKVITEISARGLPQHVPNLVAHDEKQYRRMEELFRRCEELALQHGVELHLPS
                     LSPRNKRRCDFIEEGSAFVAASGTVHPCYFLWHSFTCHADGRIRPVDALSFGSVNETP
                     LLDIWNGPEFSDYRSEIGRYDFPHCGNCSLAPCDYIERHDFEQDCLGNSLTCGSCPWS
                     VGVLQCLR"
     gene            complement(1223..1627)
                     /locus_tag="PK849_14160"
     CDS             complement(1223..1627)
                     /locus_tag="PK849_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67322.1"
                     /translation="MLILERFFLWIARWEGIPGFLGSLEVWTNMLCSATDILFVFWFL
                     RIADIARAEQGRKAIRLRYSILAACLLLTPALFLFKGPVLMIWTSLILGIPYILLAGS
                     VFFEIPGIVKLVRRRIHKAPPSGEFSGPDGAL"
     gene            complement(1614..>1798)
                     /locus_tag="PK849_14165"
     CDS             complement(1614..>1798)
                     /locus_tag="PK849_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67323.1"
                     /translation="APALSVATRHSLTLREWEYICLFYFRLVNTCYLLLEGAMAIVVF
                     LLWRNLRKEEAFDAHS"
BASE COUNT          411 a          531 c          520 g          336 t
ORIGIN      
        1 gtccggcccc ggaataccgg acgagcacat gaccatgagc acgtttcaac gcctcgtccc
       61 ggcattccct atgctggaga cattactgct caacggcatc ggcgagcccc tgcttcaccc
      121 gaggctcgag gctttcgtcc agctggcgaa ggaacatcta caggagaaag ctcgcgtcgg
      181 ttttcagacg aacggcacgc tgctcactcc ggagcgtgcg cgctcgctcc ttgccgcagg
      241 aaccaacatc gtctgtctct ccgtggacgc gacggacccg gcgctctttc agagcatgcg
      301 ggaaggagga gaaaccgagg atgtgaatcg tgccgccgcg gccctgaacg cggcgcgtgc
      361 acgcgcctcc tccccggatt tccactgggg ggctgaaatc gtgctcaccc gggacacact
      421 ggacgagctg ccccgggtgg tcgagtgggt aggggagcga gggggagcgt tccttctggt
      481 aacgcacctt cttccataca ggcaggagtc ggtaccaagc gttgcgtaca accccaacgt
      541 ggatgaaacg cttgctttct acagagagaa gagcagagag gcaaatgaac aggggattga
      601 cctctcccgt tacatcgagg ccagatggag attccgtccc gtggacaatg gtgaaagaac
      661 ggttgctttc atggagaagg taatcactga aatatctgcg cgcggactgc cccagcacgt
      721 ccccaacctc gtcgcgcacg acgaaaagca gtatcggcgg atggaggagc ttttccgccg
      781 atgtgaggag cttgcgttgc agcacggagt ggaacttcat ctcccctcgc ttagcccgcg
      841 caataaacgg cggtgcgact tcatcgagga ggggagcgcg tttgtcgccg cctccgggac
      901 ggtgcacccc tgctacttcc tgtggcactc cttcacgtgt catgcggacg gtcgaatcag
      961 gcccgtcgac gcgctctcct tcggatcggt gaacgaaacg ccgctcttgg atatatggaa
     1021 cggcccggag ttctccgact accgcagcga gatcggacgg tacgacttcc cccactgcgg
     1081 caactgcagc cttgcgccgt gcgactatat cgagcgccac gatttcgagc aggattgcct
     1141 ggggaactcc cttacctgcg gatcgtgccc atggagcgtc ggcgtgcttc agtgtctccg
     1201 gtaacctttt tctctccgca ctttacaggg ctccgtccgg gccggagaac tctccggagg
     1261 gcggagcctt gtgaattctc cggcgcacga gcttgacaat gcccggaatt tcaaagaaga
     1321 cgctcccggc gagcagtatg tacggaatgc cgaggatgag ggacgtccat atcatcagta
     1381 caggcccttt gaataaaaag agcgcggggg taagcagtaa acaggctgca agaatagaat
     1441 accggagacg gattgctttg cgcccctgct ctgcacgggc gatgtcggca attcgcagaa
     1501 accagaagac gaagagaata tccgtcgcac tgcagagcat attcgtccac acttccagcg
     1561 aacccaaaaa accgggaatg ccctcccatc gcgcaatcca cagaaaaaaa cgttcaagaa
     1621 tgagcatcaa acgcctcctc cttccgcaga tttctccata gcagaaatac gacaatcgcc
     1681 atggctccct ccagaaggag atagcatgtg ttcacgagcc ggaaatagaa gagacagata
     1741 tactcccatt cccgaagagt cagcgaatga cgtgtagcga ctgaaagtgc cggggcaa
//
LOCUS       DAOPKU010000612         1798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_77362_length_1798_cov_3.823867, whole genome shotgun sequence.
ACCESSION   DAOPKU010000612 DAOPKU010000000
VERSION     DAOPKU010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77362_length_1798_cov_3.823867"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK849_14170"
     CDS             complement(<1..282)
                     /locus_tag="PK849_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HPE67324.1"
                     /translation="MIPVIRVDERDNVAIPVADLPEGSEAGWGVVAREQIPQGHKIAL
                     EEIPEGGAVIRYGVALGHLLAGVPQGGLITEKMLRLPDSPDLDSQADEAS"
     gene            complement(279..1124)
                     /locus_tag="PK849_14175"
     CDS             complement(279..1124)
                     /locus_tag="PK849_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPE67325.1"
                     /translation="MRDDAIGADLSREIRGFRFSDFKGQVVNLFRSAFIGTFVGILPG
                     VGGSAASLLSYSQAKNFSGRPEKYGTGCVDGLVASEAANNGLTGGALIPLLSLGIPGD
                     STTAVLVGAFMLQGVQVGPLFITNNLDLWHVILSALLLCNFLMFFVMFYPIKYIASVI
                     KLPKYRIFPVIILLCVIGAYSSRNGNMFDVWTMLFFGLLGYFFVKIKLPIVPFLIGFI
                     LGKDLEKYFVDSIKGVGGSLSVFFTRPMGWVIWGLIVASIAWAVYDSKREARRAAAGN
                     GGAQA"
     gene            complement(1106..>1798)
                     /locus_tag="PK849_14180"
     CDS             complement(1106..>1798)
                     /locus_tag="PK849_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPE67326.1"
                     /translation="CRKEEETHMIDFGLLHLLMAFVGVVVGIVFGALPGMTATMAIAV
                     FLPLTYAYDLHVSLFLLLGLYVGGISGGLVPAILVNIPGTPSSICTGFDGYPMAKRGE
                     AVRALRIGITASLFGGLFSLLLLFLFTPPLARVALSFSSVEKFLIILFALSIIAALSK
                     GNMVTGVFAGFLGVFFSLIGQFADNNRMRMVPDFLSVELRNGFQLLPVLIGPSPSCRS
                     CRRRRGGCGTTP"
BASE COUNT          409 a          538 c          576 g          275 t
ORIGIN      
        1 agatgcttcg tctgcctgac tatcgaggtc gggagagtca ggcagacgaa gcatcttctc
       61 ggtgatcagt ccgccctgcg gaacgcctgc gagcagatgc ccgagcgcca cgccataccg
      121 gatcaccgcg ccaccctcgg ggatttcttc cagagcgatc ttgtgcccct gggggatctg
      181 ttcacgggcg accacacccc atccggcttc cgaaccttcg ggaaggtcgg cgacggggat
      241 ggcgacgttg tccctctcgt ccaccctgat gaccggtatc atgcctgcgc gcctccgttt
      301 ccggccgcgg cgcgccgggc ctctcttttc gagtcgtaaa ccgcccacgc gatggaggcg
      361 acgatcaacc cccagatcac ccaccccatc gggcgcgtga aaaagaccga gagggagccg
      421 ccgaccccct tgatggagtc gacgaagtac ttctccagat ccttcccgag gatgaagccg
      481 atgagaaagg gaacgatcgg gagcttgatc ttcacgaaga aataccccag gaggccgaaa
      541 aagagcatgg tccacacgtc gaacatgttt ccgttccgcg aagaatacgc accgatgacg
      601 cagaggagga tgatcacggg gaatatcctg tacttgggaa gcttgatgac cgaagcgatg
      661 tacttgatag ggtagaacat gacgaaaaac atcagaaagt tgcacagaag aagcgcggag
      721 aggatgacgt gccagaggtc gaggttgttc gtgatgaaga gcggcccgac ctgcactccc
      781 tgaagcatga aggcgccgac gagcaccgcc gtggtgctgt ccccggggat gccgagggag
      841 agcagcggaa tgagcgcgcc tcccgtcagt ccgttgttgg cggcttcgct ggcgaccagc
      901 ccgtcgacgc agcccgtgcc gtacttctca gggcgcccgg agaaattttt cgcctgcgaa
      961 tacgagagca acgacgccgc gctccccccc actccgggaa ggatgccgac gaaggtgccg
     1021 atgaacgccg accggaacag gttgaccacc tgccccttaa agtccgagaa cctgaaaccg
     1081 cgaatttcgc gcgagagatc ggcgcctatg gcgtcgtccc gcatcccccc ctccgcctcc
     1141 tgcaagatct gcacgagggc gaagggccga tgagcacggg aagcagctgg aagccgttgc
     1201 gcagctctac ggagagaaag tccgggacca tgcgcatcct gttgttgtcg gcaaactggc
     1261 cgatcaggga gaagaagacg ccgaggaacc ccgcgaagac gcccgtgacc atgttcccct
     1321 tggagagggc ggcgatgatc gagagcgcga agaggatgat caggaacttc tccaccgagg
     1381 aaaagctgag ggcgaccctg gcgagcggcg gggtgaagag aaagagcagc agcaggctga
     1441 agagaccgcc gaagagggag gcagtgatgc cgatacggag cgccctgacc gcctcgccgc
     1501 gcttggccat cgggtagccg tcgaacccgg tgcagatgga ggacggggtt cccggaatgt
     1561 tcacaaggat ggcgggaaca agccctccgc tgattccccc cacgtagagc ccgagcagga
     1621 ggaagaggga gacgtgaaga tcgtaggcgt acgtgagcgg aagaaagacc gcgatggcca
     1681 tcgtggccgt catccccggc agcgcgccga agacgatgcc gacgaccacg ccgacgaacg
     1741 ccatgagcag atgaagaagg ccgaaatcga tcatgtgcgt ttcctcctct ttacggca
//
LOCUS       DAOPKU010000613         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_77416_length_1797_cov_4.307692, whole genome shotgun sequence.
ACCESSION   DAOPKU010000613 DAOPKU010000000
VERSION     DAOPKU010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77416_length_1797_cov_4.307692"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PK849_14185"
     CDS             complement(<1..230)
                     /locus_tag="PK849_14185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPE67327.1"
                     /translation="MESHSPRFWELFFEVFEDLPRQGPGSKTCAAEALALCRELPESP
                     SILDLGCGVGGQTLYLAALRDCGFEPVGHFPLP"
     gene            complement(270..1154)
                     /locus_tag="PK849_14190"
     CDS             complement(270..1154)
                     /locus_tag="PK849_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPE67328.1"
                     /translation="MKTYKYYKLNAFVKGNSEGNPAAFLDLGDETLSDESMQRIAKEH
                     AGFVSEVVYARKNEEREIFLTYYSSECEVAFCGHGTIATMYEIIRRDEALRSAKVIEI
                     CTNKKGRLNIYNHIEEEDCIYISAPDPVMHGCTLSRASIAEALGIREEAISDALPVDI
                     IDAGLRTLLVPIRALEQEVSMYPDEQALKSYCLRNDVDIVLVFSMETAEPGYFAHTRV
                     FAPKFGYLEDPATGSGNSAFGYYLLKNKKWSGDAVRIEQGGDNRIFNEVKLMTTDGKV
                     LFGGKAALKIEGNYFVGS"
     gene            complement(1151..>1797)
                     /locus_tag="PK849_14195"
     CDS             complement(1151..>1797)
                     /locus_tag="PK849_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPE67329.1"
                     /translation="GKLSRKEEAEAFAEDVVLLRNQVLAFDLPEHGERKGENYPCTVQ
                     NGVHDLRTVYDSVKDSYSSVSLFACSLGAYFSLVAYRDVVFDKCLFLSPLLDMERLIR
                     NMMHWANVDETQLQKEREIPTSFGETLSWDYYEYVRAHPVDRWDSPTSILYGENDNLT
                     ERCVLDAFAQKFACGVTVMSGGEHYFHTPEQLAFLKNWIREQNGDGGISKEGKR"
BASE COUNT          323 a          545 c          515 g          414 t
ORIGIN      
        1 gggaggggga agtgtccgac cggctcgaat ccgcagtcgc gcagcgcggc gaggtacagc
       61 gtctgcccgc cgacgccgca ccccagatcg aggatcgagg gggattccgg cagttcgcgg
      121 cacagggcga gggcttcggc ggcgcatgtt ttgctgccgg gcccctgacg cggcaggtct
      181 tcgaagactt caaagaagag ctcccagaag cgcggtgagt ggctctccat gatcattctc
      241 ctcctcgttc cgccccggct ccagggcgct tagctgccga caaaatagtt cccttcgatt
      301 ttcagcgcgg cttttccgcc gaacaggacc tttccgtccg tcgtcatcag cttgacttcg
      361 ttgaaaattc tgttgtcgcc gccctgctct attctgacgg cgtccccgct ccacttcttg
      421 ttcttgagca ggtagtagcc gaacgcgctg ttccccgacc ctgtggcggg gtcctccagg
      481 tagccgaact tcggggcgaa cactcgggtg tgggcgaagt acccgggctc cgccgtctcc
      541 atcgaaaaga ccagcacgat atccacgtcg ttgcgcaggc agtaggattt cagagcctgc
      601 tcgtcggggt acatcgacac ctcctgctcc agagcgcgta tcggcacgag cagcgtccga
      661 agccccgcgt cgatgatatc gacgggcagc gcatccgaaa tggcctcttc acggatgccc
      721 agggcttcgg cgatcgacgc ccgtgaaagt gtgcatccgt gcatcaccgg atccggcgcg
      781 gagatataga tgcagtcctc ttcttcaata tgattgtaga tgttcagcct gcccttctta
      841 tttgtgcaaa tttcgatgac cttcgcgctt ctcagggcct cgtcccgccg gatgatttcg
      901 tacatggtcg ctatcgttcc gtgcccgcag aacgcgactt cgcactccga ggaatagtag
      961 gtcagaaaaa tctcccgctc ctcgtttttt cttgcgtaca ccacttccga cacgaagccc
     1021 gcatgctcct tcgcgatgcg ctgcatgctc tcgtcggaga gcgtttcgtc gccgaggtcg
     1081 agaaaagcgg ccggatttcc ttcggagttc ccctttacga aggcgttgag cttgtagtat
     1141 ttatacgttt tcatctcttt ccctccttgg aaatgcctcc gtcgccgttc tgctcacgta
     1201 tccagttctt caggaaagcg agctgctcgg gagtgtggaa gtagtgctcg ccgccgctca
     1261 tgaccgttac tccgcaggcg aatttttgcg cgaaggcgtc aagaacgcat cgttccgtca
     1321 gattgtcgtt ctctccgtag agtatcgagg tcgggctgtc ccagcggtcg accgggtgcg
     1381 cccgaacata ttcatagtag tcccaggaga gcgtctcgcc gaaggatgtc ggtatctcac
     1441 gttccttttg caactgcgtt tcatccacat tcgcccagtg catcatgttc cggataaggc
     1501 gttccatgtc gagcagcggc gagaggaaga ggcacttgtc gaaaacgacg tctctgtagg
     1561 cgaccaggct gaagtaggcg ccgaggctgc atgcgaacaa agagacgctg ctgtacgagt
     1621 cttttacgga atcgtacacg gttcgcaggt catggacgcc gttctgaacg gtgcaggggt
     1681 agttttcccc cttccgctcg ccgtgctccg gcaggtcgaa ggcaaggacc tgatttcgca
     1741 ggaggacgac gtcctccgcg aacgcttccg cctcttcttt ccgggagagc ttcccgt
//
LOCUS       DAOPKU010000614         1790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_77869_length_1790_cov_3.321614, whole genome shotgun sequence.
ACCESSION   DAOPKU010000614 DAOPKU010000000
VERSION     DAOPKU010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77869_length_1790_cov_3.321614"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..>1790
                     /locus_tag="PK849_14200"
     CDS             273..>1790
                     /locus_tag="PK849_14200"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPE67330.1"
                     /translation="MKTILPSLRNFPVEELTDSMVHSLEETAAKCRINAVTMVTLPNS
                     GHPAGSLSSMEMYLTVYGVADSTPGNCSSFDRDYVAISHGHTSPGAYAALAAWGFFDP
                     MDAVAHFRQCGSPFQGHVERDVPGIDWGSGNLGQGLSAGVGFALAQRARGNGKRTYVL
                     MGDGGQTKGQSAEARRIASKEKLSNITALVDWNRIQISGSLESVMPADIPALWKADGW
                     DVLECDGHSFRELYAALKQASESAVPTVILCRTVMGKGVPFMENSSEFHGKAPLNDLY
                     VQAMRDLGGDPSLLEDARNRRTSAQLPTGRHISTSSFGLDTGVPRIYTADAKKTDNRS
                     AFGSALADIGKLNYKKEGGAPLLVFDCDLAGSVKTGEFASLCPEWFIQTGIQEHATAT
                     VAGAASTAGVAALWADFGVFGLSETYNQQRLNDINKANLKTVLTHVGLDVGEDGMTHQ
                     AIDYVSLLRNTFGWKLAVPCDPNQTDRATRWAIKEPGCVCLAMGRSKLDVLTSAKG"
BASE COUNT          386 a          529 c          511 g          364 t
ORIGIN      
        1 tctcgggcgc cagggggagg aagtgatcac tccggaagag atggcttccg ccagaaatac
       61 tatcaactat gaagtgccca ttatgtttct gaaaagggtt cccagggtct acgtctccta
      121 aagaagaagg ggggcgattc caggcgggac cgtcccctcc acctcgtcgc actccaaaac
      181 acggttttct cgcctctttt ttcctctttt tttacctctt ttttctcatc ttgagtataa
      241 ttgtcctgtc acaactcaag gaggcgatct caatgaaaac aattcttcca tctctgagga
      301 acttccccgt ggaggaactc accgattcca tggttcattc tcttgaagag actgcggcga
      361 agtgccgcat caacgccgtc acaatggtaa cgctgccgaa cagcggacac cctgcgggct
      421 ctctctccag catggagatg tacctgaccg tgtacggcgt cgccgacagc acgccgggaa
      481 actgctcctc tttcgaccgc gattacgttg ccataagcca cggccatacc tctcccgggg
      541 cctacgcggc gcttgcggcg tggggctttt tcgaccccat ggacgcagtg gcgcacttcc
      601 gtcagtgcgg ctctcccttc caggggcatg ttgagcgcga cgttcccgga atcgactggg
      661 ggagcggaaa cctcggacag gggctttccg ccggcgtcgg tttcgctctt gcacaacgcg
      721 cgcgcggaaa cgggaagcgg acctacgtcc tgatgggcga cggaggacag acaaaaggcc
      781 agagtgcgga ggcgcggcgg attgcctcga aggagaaact atcgaatatt acggcgttgg
      841 tggactggaa ccgcatccaa atttcaggtt ccttggaatc ggtgatgccc gccgatattc
      901 ccgctctatg gaaagccgac gggtgggatg tgctggagtg cgatggacac tctttccgcg
      961 aactttacgc cgcactgaag caggcctcgg aaagcgcagt acccacagtt atcctctgcc
     1021 ggacagtcat gggcaagggc gttcctttca tggaaaacag ctccgaattc cacgggaaag
     1081 cgcctctcaa cgatctctat gtccaagcga tgcgcgatct cggaggcgat ccgtcgttgc
     1141 tcgaggacgc gaggaacaga agaacttcag cccagcttcc aacggggagg catatttcga
     1201 cttcctcctt cggtctggat acgggcgtac ctcgcatcta taccgccgac gcgaagaaga
     1261 ccgacaaccg gtccgccttc ggttccgcgc tcgccgatat cggaaagctg aactacaaga
     1321 aggaaggagg cgcaccgctt cttgtcttcg attgcgacct tgccggttcg gtgaaaacag
     1381 gtgaattcgc ctccctctgc ccggagtggt tcatccagac aggcatccaa gaacacgcaa
     1441 cggcaacggt tgcaggggcg gcctctacag ccggagttgc ggccctctgg gccgatttcg
     1501 gcgtcttcgg actttccgag acctacaacc agcagcgcct gaacgacatc aacaaggcga
     1561 atctgaaaac agtcctcaca catgtcggac ttgacgtcgg cgaagacggc atgacgcacc
     1621 aggcgataga ttacgtcagt ttgcttcgga acaccttcgg ctggaagctc gcggttccgt
     1681 gcgatccgaa ccagaccgac cgcgcgacac gctgggcaat caaggaaccg gggtgcgtct
     1741 gtctcgccat ggggcgaagc aagctcgatg tcctgacgag cgccaagggg
//
LOCUS       DAOPKU010000615         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_78289_length_1783_cov_3.734954, whole genome shotgun sequence.
ACCESSION   DAOPKU010000615 DAOPKU010000000
VERSION     DAOPKU010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78289_length_1783_cov_3.734954"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PK849_14205"
     CDS             complement(<1..672)
                     /locus_tag="PK849_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67331.1"
                     /translation="MLPWRRFASLSVMLAFLLIPGVLFGNDSSFMSRNLLRTLSLSPS
                     DNASVFVRRSGDILAVRTSDDRFLVYDLALGRELYREAGFTAPTFELFGEHVLFTPSG
                     ASEMRLLNARTGVVRVLPYRFKRRLTNDGVVLARSGHVLELPSGNVLFQRETITNQTV
                     FAVGDRLFFPKADRRRNYAGYDVLSKNGTLLFSLDDTPSHLLDFHDDAAFQRPFRPLS
                     FPLPLV"
     gene            complement(860..>1783)
                     /locus_tag="PK849_14210"
     CDS             complement(860..>1783)
                     /locus_tag="PK849_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPE67332.1"
                     /translation="TGALILAFVFGGGIKSFSRLGEAKIIFLYMVMLLCAGVVLVQGW
                     TPGAIIEALPSEPFFNILSRGAGKDLGSFASLLCGVLCGQIYIQAVYTASSNATAKKG
                     CLWAAFIIPPMGFLGTWIGLSMRASGVAVEASQALPFFIQTSFHPVLGGLLWSGIMIT
                     VLGTAVGVSLGVATNLTRDIFLKMKVAAHFDDVRILLASRVIVLAVVAAAGTVACASR
                     NSMILQWAYLGMGVKASGIFCLMLAAVLRPGRLSHAWSFAAGIGGLLGVAGGAFVGGG
                     LDPLFPGLALSGLVVLVGLSRGRAEALPWDC"
BASE COUNT          433 a          537 c          559 g          254 t
ORIGIN      
        1 cacaagcgga agaggaaacg aaagcggacg gaagggcctt tggaaggcag catcgtcgtg
       61 aaaatcgagc aaatgcgagg gagtgtcgtc gagagagaaa agcagcgttc cgttcttcga
      121 aagcacgtcg tacccggcgt agttcctgcg gcgatcggcc ttggggaaaa aaagcctgtc
      181 gccgacagcg aacacggtct gattcgtgat cgtctcccgc tggaagagaa cgttccccga
      241 aggcagctca agcacatgac cgctgcgcgc gagcacaacg ccgtcgttgg tcagcctcct
      301 cttgaagcgg tagggaagga cccggacgac gcccgttcgc gcgttcaaaa gcctcatctc
      361 cgacgcaccg gagggggtga agaggacgtg ctctccgaag agctcgaatg tgggggccgt
      421 gaaacctgct tcccggtaca gctcccgacc aagggccagg tcgtacacga ggaagcggtc
      481 gtcgctcgtc cggacggcga ggatgtcgcc cgagcgccga acaaagacgg aagcgttgtc
      541 ggaaggagag agagaaagcg tgcgaagcag attgcgggac atgaaggaag aatcgttccc
      601 gaaaaggact ccgggaatga ggagaaatgc cagcatgacg gacagggaag caaagcgcct
      661 ccacggcaac acggtctcac cccctagcag gcagcttcaa gagacccgtt tgttcggatg
      721 ataccgcaga gacgcttttt agtcaaatag ttttgagtag tgcaaattat agattacgct
      781 tcattcccgc agacactgaa tcgcccccgg acgtagaaac cggagaaact cccgttatga
      841 gacgcggaaa cgaatttctt caacagtccc acggaagcgc ttccgcgcgc cctctcgaca
      901 gaccgaccag cacgacgagc ccggagaggg cgagtcccgg aaagagcggg tccaggccgc
      961 cgccgacgaa cgcgccccca gcgacgccga gaagaccgcc gatccccgcg gcgaagctcc
     1021 acgcatgcga aagcctcccc ggccggagca ccgcggcaag catcaggcag aagatgccgg
     1081 aggccttgac ccccatcccg aggtaggccc actggagaat catcgaattc cgcgaggcgc
     1141 aggcgaccgt tcccgccgcc gcaaccacgg cgagcacgat gacgcggctg gcgagcagga
     1201 ttctcacatc gtcgaagtgc gccgcgacct tcatcttgag gaagatatct ctcgtgaggt
     1261 tcgtcgcgac gccgagcgag acgccaacgg ccgttccgag gacggtgatc atgatccccg
     1321 accagagcag accgccgaga acggggtgga aggaggtctg aatgaagaaa ggaagcgcct
     1381 gcgaggcctc gacggccaca ccggaagcgc gcatggagag ccctatccac gtgccgagaa
     1441 agcccatcgg cggaatgatg aaggccgccc agaggcatcc ttttttcgcc gtcgcgttgg
     1501 acgatgcggt gtagacagcc tgaatgtaga tctgcccgca gagcacgccg cacagcagcg
     1561 aggcgaacga gccgagatcc ttccccgctc cccttgagag gatgttgaag aagggttcgg
     1621 acggaagcgc ctcgatgatc gcccccggag tccagccctg aacgagcacc acgcccgcgc
     1681 acagaagcat caccatatag aggaagatga tcttcgcctc gccgagcctg ctgaaactct
     1741 tgatgccccc gccgaagacg aacgcgagga tcagcgcgcc ggt
//
LOCUS       DAOPKU010000616         1777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_78679_length_1777_cov_3.765970, whole genome shotgun sequence.
ACCESSION   DAOPKU010000616 DAOPKU010000000
VERSION     DAOPKU010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78679_length_1777_cov_3.765970"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="PK849_14215"
     CDS             <1..1666
                     /locus_tag="PK849_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPE67333.1"
                     /translation="PLNNTLALRLRDQEEEEHRILRKLTSDILARERPILDAQDVLAE
                     FDLLYGGAEVLSKRRWKLPRIEEKTSFSLHQARHPLLGEKAVPVNIKCGEGFRSLVIT
                     GPNTGGKTVVLKTAGVCIYLAWCGLPIPAEEDSRVGNIGAIFADIGDEQSIEQNLSTF
                     SAHVKNIISILEQADRTSLVLLDELGAGTDPQEGAALGVALLDTLTKEKGLTLATTHH
                     NPVKQYALTAPGVETASMEFDVETLSPTYRMLLGVPGKSNALFIAQRYGMPSAVLDKA
                     RKVLSEREVPVEELIGELNERRAWLDKEENEIRALRRTLAEERKKYQDSSRDIESRKD
                     RILAEAERKAESILERAEESSKELLRNVHDAAKSAVHRKISETSERVRNERKTLEQRQ
                     ERRALRGAAMQEGFAPVVGAVAQIAGTDYVGVIEKLQGEKAILRIGAIKMDVNAKKLV
                     KTEKKPKGASLPRDTSMEETPAAVPSSIMVRGMNVQDALPMVERYLDQALRRGHTSVT
                     VIHGRGEGILRREIHALCASLKYVESYRLGDAGEGGYGVTIVTFRR"
BASE COUNT          419 a          500 c          561 g          297 t
ORIGIN      
        1 gccgctcaac aacacgctcg ccctcaggct gcgcgaccag gaagaggagg aacatcgtat
       61 tctccggaag cttacctcgg acatactggc gcgggaacgt ccgattctcg acgcgcagga
      121 tgtgcttgcc gagttcgacc tcctctacgg cggcgccgaa gtgctctcca aacgccgctg
      181 gaagctcccc cggatagaag agaagacatc tttttcactc caccaggcgc ggcatcccct
      241 cctcggggaa aaggccgtcc ccgtgaacat caagtgcggc gaaggatttc gaagccttgt
      301 gatcacaggc ccgaatacgg gcggaaagac ggtcgtcctc aaaaccgcgg gcgtctgcat
      361 ttacctcgcg tggtgcggtc ttccgattcc ggcggaggag gactccaggg tggggaacat
      421 cggcgccatt ttcgcggaca tcggcgacga gcagagcatc gagcagaacc tgtccacctt
      481 cagcgcccac gtcaagaaca ttatctcgat tctggaacag gccgaccgga cgtcgctcgt
      541 ccttctcgac gaactcggcg cgggcaccga ccctcaggag ggcgcggccc tcggagtggc
      601 gctgctggat acgctgacga aggagaaagg gctcaccctg gcgacgacgc atcacaaccc
      661 tgtaaaacag tacgcgctga ccgctcccgg cgtcgagaca gcgagcatgg aattcgacgt
      721 ggagacactt tcacccacct acagaatgct gctcggcgtt cctggcaaga gcaacgcgct
      781 ttttatcgcg cagcgatacg gtatgccttc cgccgtcctc gacaaggcgc ggaaggtact
      841 gtccgagcgt gaagtcccgg tggaagagct catcggcgaa ctgaacgaac ggagggcgtg
      901 gctcgacaag gaggagaacg agatacgagc gctccgaaga acgcttgcgg aagagcggaa
      961 aaaataccag gactcctccc gcgacatcga atcaaggaaa gaccgtatcc tcgccgaagc
     1021 ggagcggaag gcggaaagta tcctcgaacg ggccgaggag tcgagcaagg agctcctccg
     1081 gaatgtgcac gacgcggcga agtcggcagt gcacagaaaa atttcggaga catcggaacg
     1141 cgtcaggaac gaacgaaaaa cactggaaca gcgccaggaa cgaagagcgt tgagaggagc
     1201 cgcgatgcag gaggggtttg cgcctgtagt gggggctgtc gcgcaaattg cgggaacgga
     1261 ctatgtcggc gttattgaaa aactccaggg ggagaaggcc attctccgca tcggggcgat
     1321 caagatggac gtcaacgcga aaaagcttgt caaaacggag aagaagccca aaggggcgtc
     1381 cttgcctcgt gatacttcga tggaggagac cccggcggcg gttccttcgt ccatcatggt
     1441 cagagggatg aacgtgcagg atgcgttgcc catggtcgag cggtaccttg accaggcgct
     1501 ccggcggggc cataccagcg taaccgtgat ccacgggaga ggggaaggga ttctgcgccg
     1561 cgagattcac gcgctgtgcg cctccctgaa gtacgtggaa tcctaccgtc tcggcgatgc
     1621 cggcgaggga gggtacggcg tcactattgt gacgttcaga cgctgatgca gcatacaaca
     1681 gcatcgactc gtttgtagcg atcctccccg aaatgtcaac ctgcgacatc gtatgaagga
     1741 tacgttttga gcatcggctg gaggatttaa tgaaggt
//
LOCUS       DAOPKU010000617         1776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_78731_length_1776_cov_4.720511, whole genome shotgun sequence.
ACCESSION   DAOPKU010000617 DAOPKU010000000
VERSION     DAOPKU010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78731_length_1776_cov_4.720511"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..239
                     /locus_tag="PK849_14220"
     CDS             45..239
                     /locus_tag="PK849_14220"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HPE67334.1"
                     /translation="MPVVNVQMFEGRSVEEKRKFVADVTRTICDSLGVAPESVRIMIT
                     DMPRENVAVGGVLNIDKAGK"
     gene            243..551
                     /locus_tag="PK849_14225"
     CDS             243..551
                     /locus_tag="PK849_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67335.1"
                     /translation="MNRQVFWEKLKSTLHNTWEFIVLFHHGTFVDKRMAVVRKEAFDI
                     NDNLMLLLFGDYLGVPNPMSYYMLELLPYVANDLEPWERRIQNRKFLIAEKAAQYDFD
                     "
     gene            565..1512
                     /locus_tag="PK849_14230"
     CDS             565..1512
                     /locus_tag="PK849_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HPE67336.1"
                     /translation="MRRFTFFGGKGGTGKTSCAAAYALSLASKGTRTLVVSTDPAHSL
                     ADAFGKPIGFETALLRENLWGLEIDAEVEAKKYMKVIQEKVLDLVSAAIVEEIKRQIE
                     IAYMSPGAEEAAIFDKFVELMESAGTTYDSIVFDTAPTGHTLRLLTLPEILETWIEHL
                     IEKRRKAMHLMKMAARYDSVLADKIKEDPIIETLSARKEKFALARRYLTDSSLSGFFF
                     VLNAEKLPILETARAVELLGKHGVHVGGMVINKVIPPEAGAFFEKRRSAQEGYLADIN
                     SRFSSFVRVELPLLDSDIQGVEQLEMMVPLLGALDASGE"
     gene            1520..>1776
                     /locus_tag="PK849_14235"
     CDS             1520..>1776
                     /locus_tag="PK849_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPE67337.1"
                     /translation="MKLKIACVQMRARSSEHRGEALQSALRMIDSAADGGAKMALLPE
                     TFYPSYYLGGIDPAWSWREAFSALSEKAAERRIFLAAGIVL"
BASE COUNT          388 a          477 c          544 g          367 t
ORIGIN      
        1 tgaggccagg ccttgtttca ttttaacccg aggaggtttt ttcgatgccg gtcgttaatg
       61 tacagatgtt cgaaggcaga agtgtggagg agaagagaaa attcgtcgcc gatgtcacgc
      121 ggaccatctg cgactctttg ggagtcgctc cggaaagcgt tcggattatg ataacggata
      181 tgcccaggga gaacgtcgcc gtaggcggag tgctcaacat agacaaagcc ggcaagtagc
      241 cgatgaacag acaggttttt tgggaaaagc tgaagtcaac gcttcacaat acgtgggagt
      301 tcatcgtcct cttccaccac gggacgtttg tggacaagcg tatggcggtt gtccgcaagg
      361 aggcgttcga tatcaacgac aatctgatgc ttttgctctt cggcgactat ctcggagtgc
      421 ccaatccgat gtcgtactac atgctcgaac tccttccgta cgtggcgaac gacctggaac
      481 cgtgggaacg gcgcatccag aaccgcaagt tcctcatcgc cgagaaagcg gcgcaatacg
      541 atttcgactg aggggagaac cctgatgaga cgatttacat ttttcggcgg caagggagga
      601 acgggaaaga cctcctgcgc tgccgcctat gctctttcac tcgcaagcaa aggaacccgg
      661 acgcttgttg tttccaccga cccggcgcac tccctcgcgg atgcgttcgg caaaccgatt
      721 ggcttcgaga cggcgcttct gcgcgaaaac ctctgggggc ttgagataga cgccgaggtc
      781 gaggcgaaga agtacatgaa ggtcattcag gagaaagtcc ttgatctggt gagcgcggcg
      841 atcgtcgagg agatcaagcg ccagatcgag atagcgtaca tgtctccggg agcggaggag
      901 gcggcgatct tcgacaagtt tgtcgagctt atggagtccg cgggaacgac ctacgactca
      961 atcgtcttcg atactgcgcc gacaggccat acgctccggc ttctgacact tccagagatt
     1021 ttggagacgt ggatcgagca cttgatcgag aagcgaagga aagcgatgca cctcatgaag
     1081 atggccgctc ggtacgacag cgtcctcgcc gacaagatca aggaagaccc gatcatagaa
     1141 acactttccg cgcggaagga gaagttcgcc cttgcgcgcc gctacctgac cgactcgtct
     1201 ctttccggct ttttcttcgt cctcaacgcg gaaaagcttc cgattctgga gaccgcgagg
     1261 gcagtcgagc ttcttggaaa acacggtgtc cacgtcggcg gcatggtgat caacaaagtt
     1321 atccctccgg aggcgggcgc atttttcgag aagagacgaa gcgcccagga aggctatctg
     1381 gcggatatca acagcaggtt ctcctcgttc gtccgcgtgg aactgccgct gctcgactcg
     1441 gatattcagg gcgtcgagca gctggagatg atggttcctc ttctcggcgc gcttgatgcg
     1501 tccggagagt agcggcgccg tgaaactcaa gattgcctgc gtgcagatgc gcgcgcgttc
     1561 ctcggaacac cggggcgagg cgcttcagag cgcgctccgg atgatcgact ctgcggcgga
     1621 cggcggggcg aagatggcgc tgctgccgga gacgttctac ccttcgtact acctcggggg
     1681 gattgaccct gcctggtcgt ggagggaggc gttctcagcc ctctcggaga aagccgccga
     1741 gcggaggatt ttcctcgccg ccggaatcgt cctcaa
//
LOCUS       DAOPKU010000618         1770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_79115_length_1770_cov_4.320700, whole genome shotgun sequence.
ACCESSION   DAOPKU010000618 DAOPKU010000000
VERSION     DAOPKU010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79115_length_1770_cov_4.320700"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1770
                     /locus_tag="PK849_14240"
     CDS             <1..>1770
                     /locus_tag="PK849_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPE67338.1"
                     /translation="KHVRETKTSKAINFAGRLCSAAVLLLARLSVIPILLWLSWEFAV
                     HAALRFDIEGVPSIEELRSYRPMRTTVLYDRYGKPFARFFIEDRVELPLSGMSPWIPK
                     AVVAAEDARFPSHRGVSLAAIARAAIENQRAGTIRQGGSTITQQLARNMFLSRERTIE
                     RKAREAVIAFRLESEFSKEEILEKYLNEIYFGRGAWGVETASQVYFGKHARDLSLGEA
                     ALLAGVIPSPNRTNPGASPDAAEAGKRRVLARMFETGAISARERSEAEAPVALVPEPG
                     NLPSEHVRYPYYAMRVLNDTLLPEYGVNGAYGGGLRVYTMLDPDLQEAGERIAARSKH
                     QLAIVGLDSETGGVRALVGGKDWHVSQFDRAVQAYRQPGSAFKPLVYATLFEKKGWSG
                     ATKIQDAPISFGSGRNRWSPRNYDGKFRGSVTVERAIIGSLNVPAVRALRAVGAGVVA
                     ETVRSMGITTPYLPDNPTMALGSASLTPLEMAAAFSVFGNGGYRIVPTLVREIRDADG
                     KVLFKAAEEKKRVLSEYTANGIRDILIKAVSSGTGRAARIDGFQVFGKTGTTNDFRDA
                     WFVGGIPGFCAAVYVGDDDRKPLG"
BASE COUNT          316 a          543 c          617 g          294 t
ORIGIN      
        1 gaagcatgta cgggagacca agacaagcaa ggctatcaac ttcgcgggga ggctctgctc
       61 cgccgccgtt cttttgctgg cgcgtctttc cgtgattccc attcttctgt ggctctcctg
      121 ggagttcgcc gtccacgccg cgctccgctt cgacatcgaa ggtgttccgt ccatcgagga
      181 gctccgctcg taccgtccga tgaggaccac ggtgctctac gacaggtacg ggaagccgtt
      241 cgcgcgtttt ttcatcgagg acagagtgga gcttcccctc tccggaatgt ccccgtggat
      301 accgaaggcc gtcgtcgcgg cggaggacgc gcgcttcccg tcgcaccgcg gggtgagcct
      361 ggcggcgatc gcgcgggccg ccatcgaaaa ccagcgcgcc gggacgattc gccagggcgg
      421 cagcacgatc acgcagcagc tggcgcgcaa catgtttctc tcccgcgagc ggacgatcga
      481 gcggaaggcc cgcgaggcgg tcatcgcatt ccggcttgaa agcgagtttt cgaaggagga
      541 gatcctggag aagtatctca acgagatcta cttcggccgc ggagcgtggg gtgtggagac
      601 agcgtcgcaa gtgtacttcg ggaaacacgc gcgcgatctc tccctcggcg aggcggcgct
      661 tctggccggg gtgatcccct cccccaaccg caccaatccg ggggcgtcgc ccgacgccgc
      721 ggaagccggg aagagacgcg tgctggcgag gatgttcgaa accggcgcga tctccgcgcg
      781 cgagcgctcc gaggcggaag cgccggtcgc gctcgttccg gagccgggga accttccttc
      841 ggagcatgtg cggtatccgt actatgcgat gagggtgctc aacgacacgt tgctgcccga
      901 gtacggggta aacggcgcgt acggcggagg actgcgcgtc tacacgatgc tcgaccccga
      961 tctgcaggag gcgggcgaac gcatcgccgc gcggtcgaaa caccagctcg cgatcgtggg
     1021 gctggactcg gagaccggag gcgtcagggc gctcgtcggc ggcaaggact ggcatgtaag
     1081 tcagttcgac agggccgtcc aggcgtatcg gcagcccgga tcggccttca aaccgcttgt
     1141 gtacgctacg ctcttcgaga aaaaggggtg gagcggggcg acgaagattc aggacgctcc
     1201 gatttcgttc ggctcggggc ggaacagatg gtcgcccaga aactacgacg ggaagttccg
     1261 ggggagcgtg acggtcgagc gggcgatcat cggatcgctg aacgtcccag ccgtcagggc
     1321 gttgcgcgcc gtgggagcgg gagttgtggc ggaaaccgtc cgcagcatgg gcatcaccac
     1381 gccgtacctt ccggacaatc cgacgatggc gctggggagc gcctcgctga cgccgctcga
     1441 aatggcggcg gccttctcgg tcttcggaaa cgggggatac aggatcgtcc cgacgcttgt
     1501 tcgcgagatc cgcgacgccg acgggaaggt cctcttcaaa gcggcggagg agaagaagcg
     1561 cgttctctcc gaatacaccg cgaacgggat cagggacatt ctcatcaagg ccgtttcgtc
     1621 cggaacggga cgcgccgcgc gcatcgacgg attccaggtc ttcggcaaga cggggacgac
     1681 gaacgacttc cgcgacgcat ggttcgtcgg cggcattccg ggcttctgcg ccgccgtcta
     1741 cgtgggcgac gacgacagga agccgctcgg
//
LOCUS       DAOPKU010000619         1769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_79211_length_1769_cov_3.457993, whole genome shotgun sequence.
ACCESSION   DAOPKU010000619 DAOPKU010000000
VERSION     DAOPKU010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79211_length_1769_cov_3.457993"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PK849_14245"
     CDS             <1..482
                     /locus_tag="PK849_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HPE67339.1"
                     /translation="HPGRLLADFVDIFDFPPTVINMGVNGLLVTGYLLAVGGDFNGPT
                     LGGIFTVVGFSAMGKTPRNIAPIIAGVVLGSLTKHWSLSDPAIQLAALFGTTLAPIAG
                     EFGWKAGILAGYVHSSVVLYVGVLHAGFNLYNNGFAGGLVAAILVPLIETFRGRETE"
     gene            479..829
                     /locus_tag="PK849_14250"
     CDS             479..829
                     /locus_tag="PK849_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67340.1"
                     /translation="MKYERHVVSRIVSELMNFFFSMGARDFSSHVIRNEDRFEICVES
                     DYAGNPTTKIREMTRLLRMPRAREMEEYSWSLSGDISTGQEIYLVGIMTDDVTVEHDE
                     EAGRVRISLTRLLE"
     gene            850..>1769
                     /locus_tag="PK849_14255"
     CDS             850..>1769
                     /locus_tag="PK849_14255"
                     /inference="COORDINATES: protein motif:HMM:NF017812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 family protein"
                     /protein_id="HPE67341.1"
                     /translation="MPKLELTIDLLEEALLGGSILGGGDDSSIDDALMLGELALKINS
                     PALLDIQDIDPNGIVVTCAGVTCPHRMKAPFVNPRAHVRSIELLLESGLPRPAALIAS
                     ECGSGGIVNGWLQAAILGLPLVDAPCNGRAHPTPEMGSMGLHLAPDYQAVQAFAGGDP
                     TQGTYIEGVLRGNVTTVSAMVRQDACIVGGLLAVARNPVKAGYLQENAAPGAIKLAIG
                     LGKAVKAVRSGGGDAVTEAILSVLPGEVLCRGRVDAVDRFTAGGMDSGTAYVGEYSVS
                     FWREYMTVEHGEEERLATFPDLITVVDAETG"
BASE COUNT          351 a          500 c          577 g          341 t
ORIGIN      
        1 cgcatccggg acgactgctt gccgacttcg tcgatatctt cgatttcccg ccgacggtga
       61 tcaacatggg ggtgaacggc ctgctcgtga ccggctatct gttggctgta ggcggcgatt
      121 tcaacggtcc cacgctcgga ggcatcttta ccgtcgtcgg tttcagcgcg atggggaaaa
      181 cgccgaggaa catcgcgcct attatcgcgg gagtcgtcct cggaagtctg acgaagcact
      241 ggagtctttc cgacccggca atacagcttg cggcgctctt cggaacgacg ctcgcgccta
      301 ttgcgggcga attcggctgg aaagcgggaa tactggccgg gtacgtccac tcgtccgtgg
      361 tgctgtatgt cggcgtactg cacgccggct tcaacttgta caacaacggc ttcgccgggg
      421 gactcgtcgc ggctatcctc gtccccctca tcgaaacctt cagagggaga gaaacggaat
      481 gaaatacgaa cgtcatgtcg tctcgcgcat cgtgagcgag ttaatgaact tcttcttctc
      541 gatgggagcg cgcgactttt cgtcgcatgt catccggaac gaagacaggt ttgaaatatg
      601 cgttgaatcg gactacgcgg gcaacccgac gacaaagatc cgggagatga cccggctgct
      661 cagaatgccg cgcgcccgcg aaatggagga gtactcctgg tcgctctccg gagacatcag
      721 cacgggacag gagatctatc tcgtggggat catgacggat gacgtgaccg tggaacacga
      781 cgaggaggcg gggcgggtcc gcatcagtct cacccgtctt ctggagtgac ctgaaagggg
      841 gaggaaagta tgccgaagct tgaattgacc attgatctgc tcgaggaggc ccttctgggc
      901 ggctcgattc tcggaggggg agacgactct tcgatcgacg atgcgctgat gctcggcgag
      961 ttggcgctca agatcaacag cccggcgttg ttggatatcc aggatatcga tccgaacggg
     1021 attgtggtta cgtgcgccgg cgtgacgtgt ccccatcgga tgaaagcccc tttcgtgaac
     1081 ccgagggcgc acgtccggag catcgaattg ctgctggaaa gcggattgcc gcgacccgcc
     1141 gccctcatcg ccagcgagtg cggcagcggc ggaatcgtga acggctggtt acaggccgct
     1201 attctcggat tgcccctcgt cgacgctcca tgcaacggca gggcgcaccc gacgcccgaa
     1261 atgggctcca tggggttgca tctcgcgccg gactatcagg ccgtacaggc cttcgccgga
     1321 ggcgatccaa cccagggaac atatatagaa ggcgttcttc gcgggaacgt cacaaccgtc
     1381 tcggcgatgg tgcgtcagga cgcctgtatt gtgggcgggc ttctcgcagt cgcgcgcaac
     1441 ccggtgaagg ccgggtatct gcaagagaac gccgcgccgg gggcgatcaa actcgccatc
     1501 ggactaggta aagcggttaa ggcggttaga agcggaggag gcgacgccgt gaccgaagcg
     1561 atactctccg tgcttccggg cgaggtcctc tgcaggggca gagtcgacgc ggtcgatcgt
     1621 ttcacggcgg gaggcatgga ctccggaacc gcgtatgtcg gagagtattc tgtctccttc
     1681 tggagggagt acatgacggt ggaacacggc gaagaagagc gcctggccac ctttccggac
     1741 ctcatcacgg tcgtcgacgc cgagacggg
//
LOCUS       DAOPKU010000620         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_79663_length_1762_cov_3.992970, whole genome shotgun sequence.
ACCESSION   DAOPKU010000620 DAOPKU010000000
VERSION     DAOPKU010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79663_length_1762_cov_3.992970"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PK849_14260"
     CDS             <1..741
                     /locus_tag="PK849_14260"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPE67342.1"
                     /translation="HGCFRERESRVFSLSLPNGKATFSNAFQEPLLVVAGGRKPAASW
                     LAEIFRSFTTRWCADSGLVYCLEAGILPDRLVGDGDSTPPDEWRRAVEAGVSVTKHPV
                     DKDLTDLQLALFTAGKELHGKPVLVTGCWGGRFDHLWANIFSALWAAEQDIRILVFAD
                     EKECLFLLEGEEALEICVSGRENVVVSLLPLTPECAGVDLQNVRWELKDSLLLQKHPY
                     TVSNRTEGNKNISVRLQKGILGVYCGWL"
     gene            complement(758..952)
                     /gene="rpmB"
                     /locus_tag="PK849_14265"
     CDS             complement(758..952)
                     /gene="rpmB"
                     /locus_tag="PK849_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPE67343.1"
                     /translation="MAKVCECCGRGPTTGNAVSHSNRHTRRRWLVNLRSVKADVGGGE
                     SLRMRICTRCLRSGKVKRAV"
     gene            1064..1294
                     /gene="xseB"
                     /locus_tag="PK849_14270"
     CDS             1064..1294
                     /gene="xseB"
                     /locus_tag="PK849_14270"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPE67344.1"
                     /translation="MSFSAKMDELEGLVARIEKEALPLEDALALFEKGVGLVRECREY
                     LSEAKQRILLLTEDSSTVPFVATYNNDEESSR"
     gene            1291..>1762
                     /locus_tag="PK849_14275"
     CDS             1291..>1762
                     /locus_tag="PK849_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPE67345.1"
                     /translation="MTQNTRSFQALFSEAGEFVEARLQELAEMLPVPAPRRLRESMIY
                     SLTAGGKRLRPILCLKAAEVFGMSWDVSIPMALGFEMIHTASLIHDDLPAMDNDVLRR
                     GKATNHVLFGESLAILAGDALMAWAFEYPLSALSKSNVPAERICEAMRILANSLG"
BASE COUNT          391 a          413 c          511 g          447 t
ORIGIN      
        1 cacgggtgct ttcgggagag ggagagtagg gtgttctctc tctctcttcc caatgggaag
       61 gcaaccttct caaacgcttt tcaagagcct cttctggtcg ttgcaggggg aagaaaaccg
      121 gctgcttcct ggttagctga gatttttcgc tcgttcacga cgcgctggtg cgctgattcc
      181 ggccttgtgt attgtcttga agcgggtatt ctgccggaca gacttgtggg agacggagac
      241 agtactcctc cggatgaatg gaggcgtgct gtagaggcgg gagtttccgt aacgaaacac
      301 cctgtggata aggatttgac agatcttcag ctggcgctct tcacggcggg aaaagagctc
      361 cacggaaaac cggttctggt aaccggttgc tggggagggc ggttcgacca tctctgggcg
      421 aatatattct ccgcgttgtg ggcggccgaa caggacattc gaattcttgt atttgcggat
      481 gaaaaggagt gtctgttcct cttggagggg gaagaagctc tcgaaatttg cgtttccggg
      541 cgtgagaatg tcgtagtgtc gctgttgccg cttactccgg aatgcgccgg tgttgatctc
      601 caaaatgttc gttgggagtt gaaggattcc cttctactgc aaaaacaccc ctatacagtg
      661 agcaacagaa ccgaggggaa caaaaatatc tcagtaaggt tgcagaaggg aatccttggt
      721 gtatactgcg gatggctctg aaaagcaaaa gagggcttta caccgcccgt ttcactttgc
      781 cggaacggag acagcgtgtg cagattctca tgcgaagaga ttcgccgccg ccaacgtcag
      841 ccttgacgct ccgaagattg acaagccagc gcctgcgtgt atgccggtta gaatgactta
      901 cggcatttcc ggtggtaggc ccgcgaccgc aacattcaca tacttttgcc attgatttcg
      961 cccccttgct gcgattggtt tcgataaaac agaagaattt tagcacagag gggtgcatgg
     1021 agcaaatttt ttttgtagaa ttcctcgggg aggattggac aaaatgagtt ttagcgcgaa
     1081 aatggatgaa ctagaggggt tggtagcccg catagagaag gaagccctgc ctttggaaga
     1141 tgccctcgcc ctctttgaaa agggggttgg gttggtacgg gagtgccggg aatatctttc
     1201 ggaagcaaag cagagaattc ttctccttac ggaggatagc agtaccgtac cttttgttgc
     1261 gacctacaat aacgatgagg agtcaagccg atgacccaga atacacgttc ttttcaagcg
     1321 cttttttcgg aagccgggga gtttgtcgag gcgcgcttgc aagaacttgc cgagatgctt
     1381 cccgtccctg ccccccgcag gctcagggaa tcgatgattt attcactgac tgccggtggg
     1441 aaacgtcttc gtcccattct ctgcctgaag gccgcggagg ttttcggtat gtcgtgggac
     1501 gtgtccatac ctatggcact tggctttgag atgattcata cggcctctct cattcatgac
     1561 gatctccccg ctatggataa tgatgtttta cggagaggga aggcaacgaa tcatgtcctc
     1621 ttcggcgaat ccctcgccat actcgccgga gatgcgttga tggcctgggc gtttgaatac
     1681 cctctctctg cgctttcgaa gtcgaatgtt cccgctgaga gaatctgtga ggcaatgcgc
     1741 atattggcaa actcattggg gc
//
LOCUS       DAOPKU010000621         1759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_79880_length_1759_cov_3.377347, whole genome shotgun sequence.
ACCESSION   DAOPKU010000621 DAOPKU010000000
VERSION     DAOPKU010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79880_length_1759_cov_3.377347"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PK849_14280"
     CDS             complement(<1..691)
                     /locus_tag="PK849_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67346.1"
                     /translation="MNTCSEVFIITHAGRSIGGGHASRCSALAEGFSSLGVAVRWLVN
                     ASAGEIVLRRGATEASVTVMESPFGGGADAVFTAISRSRPALCVVDGYDATPSFLREL
                     RRFAPVVLVDDCRVRPVERECDVVLNYNMNADLLGYEQGHAGLLLGSEYALLRSEFWS
                     LAPEKGDAVLIIPGASDLLHTGERFVEWWGSDWPRAELVLGPLVESSMAERLSEVAGA
                     LPNLSVVRNPLD"
     gene            complement(1255..>1759)
                     /locus_tag="PK849_14285"
     CDS             complement(1255..>1759)
                     /locus_tag="PK849_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67347.1"
                     /translation="LALLAAKRMIESGRSDNKRIIFLKHLGSLLDERGWSRERSVALY
                     RFIEWILRPKSEKKYEEYKAWVRKEERKVYITAAEEIGMEKGLEKGVEIGKEIGKEIG
                     KEIGKEIGKEIGKEIGKGEGILIGKEDTKREAALRMLRKGMEVSLVAECVDLPEDEVR
                     RLCEERS"
BASE COUNT          386 a          578 c          414 g          381 t
ORIGIN      
        1 ggtcaagcgg gtttcgcact accgaaaggt tcggcaacgc gccggcaacc tcggacagtc
       61 gctccgccat tgaagactcc accagcggac cgaggacaag ctccgcacgc ggccaatcgc
      121 ttccccacca ctccacgaac cgttcgcccg tatgcagcaa atcgctcgcg ccgggaatga
      181 taagcacggc gtctcccttt tcgggagcca gactccaaaa ctcgctccgc agcagggcgt
      241 actcagaccc cagaagcagc ccggcatgtc cctgttcgta tccgagcagg tccgcattca
      301 tattgtagtt cagcaccacg tcgcattcgc gctcgacggg ccgtacccgg cagtcgtcca
      361 ccagcaccac cggggcgaac cggcgtagct ccctcagaaa agaaggcgtt gcgtcgtacc
      421 cgtccaccac gcagagggct ggacgagaac gtgagatcgc agtaaacaca gcatccgccc
      481 cgccgccgaa cggactctcc atcaccgtca cggacgcttc ggtagcccct ctgcgcagca
      541 caatctcccc ggcggaagcg ttcaccagcc aacggacagc aacgccgagc gaagagaacc
      601 cctcggccag cgcggaacag cggctcgcat gcccgccccc gatggaccgc cccgcgtggg
      661 taattatgaa aacttcgctg caagtattca tgtgaccgac tccgtttact ctgattttgc
      721 cgctgacagc gcttccgcat tcgcatcgcg ctgtgctttt attataccta cagtggaaaa
      781 tcttgttgtt gagcgttgtc gaggaaagaa cgcaggagag aggggagacg ttaggtgaga
      841 agagagacga gaagaagcaa aaatttaccg ttgtcgctaa gatgctgtgc aaaggtaacc
      901 ctcacgaaaa gattctggtc atagggagat atctccggag gaactcgacg agatggtcga
      961 atcgcgcaaa aactgagcgg caccctcacc cctcgtcact ctctccacat cctcccggcg
     1021 aagacggctc accaatcgat acagtttttt gctccttgac atcaacagac aatccaaaga
     1081 cctctttgtt agcaagtggc tctgcttgat tataacactc gacggcttct cgccagggaa
     1141 tacagcaagg cttaaaatga cacgcgaaag gcacgtctta aaaattcggc gttatgccgt
     1201 cacgtaatca aaagcacggc ttccagacac attctccgca cgcgacggtc gtgctcagct
     1261 cctctcttcg caaaggcggc ggacttcgtc ttccgggagg tcgacgcact ccgcaacgag
     1321 ggaaacctcc atccctttgc gaagcatcct gagagcggct tcacgcttgg tatcctcctt
     1381 cccgatgagg atgccttcgc cctttcctat ctcttttcct atctcctttc ctatctcctt
     1441 tcctatctct tttcctatct ctttccctat ctcctttccg atctccacgc ccttttccaa
     1501 tcccttctcc atgcctatct cctcggctgc agtgatgtac actttcctct cctccttcct
     1561 tacccatgcc ttgtactctt cgtacttctt ctcactcttc ggccgaagta tccattctat
     1621 gaagcggtag agcgcgacgc tgcgttcgcg agaccacccg cgctcgtcca gaagacttcc
     1681 gagatgcttc aaaaaaatga tccgtttgtt gtccgagcgc ccgctctcga tcatcctctt
     1741 cgccgcaaga agcgcgagc
//
LOCUS       DAOPKU010000622         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_79962_length_1758_cov_2.758074, whole genome shotgun sequence.
ACCESSION   DAOPKU010000622 DAOPKU010000000
VERSION     DAOPKU010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79962_length_1758_cov_2.758074"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..962)
                     /locus_tag="PK849_14290"
     CDS             complement(90..962)
                     /locus_tag="PK849_14290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPE67348.1"
                     /translation="MTNSEFSLLIGYRDIDPARFSFNELARKTFGLDFERWYRSGYWT
                     KQYIPYSLAKEGQVVANVSVSFMPVNIDGAPMTMAQIGTVMTDPAFRGRGLCRILMET
                     VLEEWTPKVDDVYLFANDATLDFYPKFGFRKSAEACSSAAVRTSGPARMQKLDMDSAE
                     NLAKVERAVRGTRGVSRLRVNNAGLVMFHLTGPLRENVYSDPLTNAIIVAEPEGENLR
                     VDDVFCEDAVNLDDAVGAFGERVKRAAFGFPPTQDENMERTRCTEVNTTLFVLKNAVD
                     FFEKGLMFPVLSRA"
     gene            complement(996..>1758)
                     /locus_tag="PK849_14295"
     CDS             complement(996..>1758)
                     /locus_tag="PK849_14295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE67349.1"
                     /translation="IGSLFLLAVFVLTGGKFSGLRNVRHLAYLILSGVAMGASWIFLF
                     EAYQQIGVGMATLAYYCGPVIVMALSPLLFKEKLTLARICGFVAVLAGMFLVNRDALM
                     VDGFSWRLLCGGLSAVMFALMVILNKMAASITGFENAVWQLATSFVTVAIYTVLRQGF
                     AIDILFGSFAPVLLLGLVNTGVGCYLYFSSMQRLPAQSVAICGYLDPLSALVFAAVFL
                     AERMSPEQMAGAALILGGAAFGEYFGRKRLPDVSW"
BASE COUNT          400 a          586 c          462 g          310 t
ORIGIN      
        1 gcgacctcct tccgttccgt cacactccgc gctcttccgc aggatcgccg ttcagcctgt
       61 cgggcaacga gaaaaagaag acggttccct cacgcgcggg agaggacggg gaacatcagc
      121 cctttctcaa aaaaatccac cgcgttcttc aggacgaaga gggtcgtatt cacctccgta
      181 cagcgcgtcc tttccatgtt ctcatcctga gtcgggggaa agccgaacgc cgctcttttc
      241 actctctcgc cgaaagcgcc gaccgcatcg tccagattca ccgcgtcctc gcagaagacg
      301 tcgtcgaccc gcaggttctc cccctcgggc tccgcgacga taatggcgtt cgtcaggggg
      361 tcggagtaca cgttctcacg caagggaccg gtgaggtgga acatgacgag cccggcgttg
      421 ttcacacgaa gcctgctcac gccgcgcgtt ccgcgtactg ctctctccac tttcgccagg
      481 ttttcggcgg agtccatatc cagcttctgc atccgcgccg gccccgacgt gcggacagcg
      541 gcggaagagc acgcttccgc gctctttcga aatccgaact tcggatagaa atcaagcgtt
      601 gcgtcgttgg cgaaaagata gacatcatcg accttcggcg tccactcctc cagcacggtc
      661 tccataagga tccggcacag ccccctcccc ctgaaggcag ggtcggtcat caccgtgcct
      721 atctgcgcca tcgtcatcgg cgcgccgtcg atgttcaccg gcatgaagct caccgacacg
      781 ttcgcgacaa cctgcccctc cttcgccaga gaatagggga tatactgctt cgtccagtac
      841 cccgaacggt accatctctc gaagtcgagc ccgaaggtct tccgcgcaag ctcgttgaac
      901 gagaatcgtg cagggtcgat atcccggtag cctatcagaa gtgaaaactc gctgttggtc
      961 atcgtcttct ccttgaacag acgaatttga cttattcacc aagaaacatc cggaaggcgc
     1021 ttcctgccga aatactctcc gaacgcggcg cctcccagaa tcagcgccgc cccggccatc
     1081 tgctccggag acatgcgttc ggccaggaaa accgcagcaa agaccagcgc cgagagcgga
     1141 tccagatagc cgcagatcgc gacggattgc gccggaagcc gctgcatcga cgaaaagtac
     1201 agatagcacc cgacgcccgt gttcacaagc cccagcagaa gaacgggcgc gaaacttccg
     1261 aagagaatgt cgatcgcaaa cccctgcctg agcactgtat agatcgccac cgtgacgaag
     1321 ctcgtcgcaa gctgccacac ggcattttcg aaccccgtga tgctcgcggc catcttgttg
     1381 aggatcacca tgagcgcgaa catgaccgcc gagagcccgc cgcacagcag tctccatgaa
     1441 aacccgtcca ccatcagcgc atcccggttg acgagaaaca tccccgcaag aacggcgacg
     1501 aaaccgcata ttcttgcaag agtaagcttt tccttgaaca acagcggcga gagcgccatc
     1561 acgataaccg gcccgcagta gtaggccagc gtcgccatgc cgacgccgat ttgctggtac
     1621 gcctcgaaga gaaaaatcca gctcgcgccc atggcgaccc ccgacaggat caagtacgcg
     1681 aggtggcgca cattccgcag cccggagaac ttcccccctg tcaggacgaa aacggcgaga
     1741 agaaacagac tgccgatc
//
LOCUS       DAOPKU010000623         1755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_80195_length_1755_cov_2.355882, whole genome shotgun sequence.
ACCESSION   DAOPKU010000623 DAOPKU010000000
VERSION     DAOPKU010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80195_length_1755_cov_2.355882"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PK849_14300"
     CDS             complement(<1..913)
                     /locus_tag="PK849_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE67350.1"
                     /translation="MKLRRIELKGFKSIDGRTGQTIPFGDITVMLGANGSGKSNIVSF
                     FKMLNFMMTGAFQSYVGRQGVQQLLFYGPRTTESIIFSLFFSSEEADDTYEARLSYGL
                     PDRLFIGGEKVTYLKKGETSAQEFFLESGGAEVRLGKDERKTSRVLFRILSGIRAYQF
                     HDTSDTAKIKSRGYVDDAAYLRSDAGNLAAFLKMLKEHSEYMKYYDRIVRHIRRVMPQ
                     FGDFSLLTVPGNEKYVRLNWTDSLGGDYLFDPDQISDGSLRFMALTTLLLQPPALLPK
                     FIVLDEPELGLHPAAIADLAGMIRTASQ"
     gene            complement(1084..>1755)
                     /locus_tag="PK849_14305"
     CDS             complement(1084..>1755)
                     /locus_tag="PK849_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE67351.1"
                     /translation="LCAGAFIGWINGLLVVVVGIPSIIATIGTQFFWRGLVLLAADGL
                     AIALPELRGETLHFLLAGRIGAWGVPAQALWAVALALLLAFLLNRRPFGDAILFIGDN
                     RNTAEMMGIPVGRVRIAVFALMGLLAAFAGILATFELNNWWPTQGEGYMLMVFASVFV
                     GGTSAYGGEGTIYGSFIGAIIIGIIEAGIVSAGLVGFWTRLVHGLVILLSVSVYALLA
                     RRRAR"
BASE COUNT          369 a          520 c          512 g          354 t
ORIGIN      
        1 tttgggacgc cgttcttatc attccggcaa ggtcggcgat agcggcgggg tgcaggccga
       61 gttccggttc gtccagtaca atgaacttcg gcagcagcgc cggtggttgg agcaaaagcg
      121 tcgtcagcgc catgaagcgg agggaaccgt cggagatctg atcgggatcg aaaagatagt
      181 ctccgccaag agagtcggtc cagttcaagc ggacgtactt ctcgttgccc ggtacggtca
      241 ggagcgagaa atcgccgaac tgcggcatca cgcgtcggat atgcctcaca atacgatcat
      301 agtatttcat gtattccgaa tgttccttga gcatcttcag gaaggcggca agattgcccg
      361 cgtcgctgcg cagataggcg gcgtcgtcca catacccgcg gctttttatc ttcgcggtat
      421 ccgatgtatc gtggaactga taggcgcgta tgccggaaag aatccggaag agcactctgc
      481 ttgttttacg ttcgtccttt ccgagacgaa cttccgcacc gccgctttcc agaaagaact
      541 cttgagcgga ggtctcgcct ttctttaaat aggtgacctt ttcaccgccg atgaacagac
      601 ggtccggaag gccgtaggag agtctcgcct cgtaggtgtc gtccgcctcc tccgaagaga
      661 aaaagaggga aaaaataatc gactcggtcg ttcggggtcc gtagaagagc aattgctgaa
      721 ctccctgtct tccaacatag ctctgaaaag ctccagtcat cataaaattc agcattttga
      781 agaacgagac aatgttgctc ttcccggagc cgttcgcgcc gagcatcacg gtgatgtcac
      841 cgaacggaat cgtctgtccg gttcgtccgt cgatcgattt gaagcccttc agttcgatac
      901 gccgtagttt catagaactc ccctccgcag cgcgctctct attcagtctc tcggtgcggg
      961 acgatacgcg cccagtcttt tctatcatat ttcccggtgt ccggcaaggc gtaccgcctt
     1021 ttaccgcgat gcctttttca cgatgttcct ccgcttttac acttcgttta tgacgattct
     1081 tcgctacctc gccctcctgc gcgcgagcag cgcgtagacc gagacggaga gaaggatgac
     1141 gaggccgtgg acgagacgtg tccagaagcc gaccagcccc gcgctcacga ttcccgcctc
     1201 gatgatgccg atgatgatcg cgccgatgaa ggagccgtag atagttcctt ctccgccgta
     1261 ggccgaggtg ccgccgacga agacggaggc gaagaccatc agcatgtacc cctccccctg
     1321 cgtgggccac cagttgttca gctcgaaggt ggccaggatt ccggcgaacg cggcgagcag
     1381 acccatcagg gcgaagaccg cgatgcggac ccgcccgaca ggaatgccca tcatctcggc
     1441 ggtgttgcgg ttgtcgccga tgaagaggat cgcgtcgccg aacggacgcc tgttgagcag
     1501 aaacgcgagc agaagggcga gcgcgacggc ccacaaggcc tgcgcgggga ctccccacgc
     1561 gccgatcctt cccgcgagca ggaagtgcag cgtctctccc ctcagctcgg ggagcgcgat
     1621 cgccagaccg tccgccgcga gcagcaccag ccctctccag aagaactgcg ttccgatggt
     1681 ggcgataatg gaggggattc cgacgacgac gacgagcagg ccgttgatcc agccgatgaa
     1741 cgccccggcg cagag
//
LOCUS       DAOPKU010000624         1754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_80232_length_1754_cov_3.892290, whole genome shotgun sequence.
ACCESSION   DAOPKU010000624 DAOPKU010000000
VERSION     DAOPKU010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80232_length_1754_cov_3.892290"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..83
                     /locus_tag="PK849_14310"
     CDS             <1..83
                     /locus_tag="PK849_14310"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HPE67352.1"
                     /translation="EFDLFSKGPDGEEGGEGAGKDITNWE"
     gene            344..715
                     /locus_tag="PK849_14315"
     CDS             344..715
                     /locus_tag="PK849_14315"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HPE67353.1"
                     /translation="MKKNFAVLLTVALVCVLSLYAFAGAAEQPLSVTGGEPVVYDCNN
                     GDEVVARYYMLSDESLRFVKLLLPDGTEYTLPQVLSGSGARYTDDIELLWWIKGDDAY
                     VEKRDETGEWRPLYSECRARP"
     gene            complement(882..1124)
                     /locus_tag="PK849_14320"
     CDS             complement(882..1124)
                     /locus_tag="PK849_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67354.1"
                     /translation="MASFPQATARVAVGDLIGKIRAHYGKSVETNIYDPRCLFWFFDL
                     VRFNIRAEPTWVFDGKLLFRGIPNWEELREKMDATL"
     gene            complement(1218..>1754)
                     /locus_tag="PK849_14325"
     CDS             complement(1218..>1754)
                     /locus_tag="PK849_14325"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPE67355.1"
                     /translation="SAFALSTMGGYSGGLRSPLRKLLDKKGYRTLGYAEFVMPSNYAN
                     KSIPQEANREKIQKCEAKAREFALALLEDRATWCSGGPFSPFFNWMARKKAPWRMMKR
                     SFPLEIDKSRCIQCGKCARLCPAKNIVMEEYPRFLDHCVVCQRCIAFCPPAAIGVHGK
                     KYEQYRAVEYDTLVSDKL"
BASE COUNT          388 a          474 c          489 g          403 t
ORIGIN      
        1 ccgagtttga tctcttctcg aagggaccag acggagaaga gggcggcgaa ggagcaggaa
       61 aggatattac gaactgggaa tagacaacta tgtgtatccg ggtcgcctca cgaagcagag
      121 gcggcctttt ttatgcgcac tcgtccataa acgggccatt gagggaaaaa accgggagaa
      181 tgtgatgtgt cgtttcacgg ggagatagcc gcaggttcac tctccaggaa cccgaatagg
      241 gctgcgaatt ttccttcttg caacgcgccc aagaagtgcg tattattgaa taacgcttca
      301 gacagccatt caaatacctt tcaggggaga aggaggggga agcatgaaaa agaacttcgc
      361 agttctgctt acggtagcgc tggtctgcgt cctttcgctg tatgcttttg cgggcgccgc
      421 ggagcaaccg ctgtccgtca cggggggcga acctgtggtg tacgattgca ataacgggga
      481 tgaggttgtc gctcggtatt acatgctctc tgacgaaagt ctccgtttcg tcaagctgct
      541 cttaccggac ggaaccgagt atacgctgcc ccaggtgctt tccggctcgg gagcccggta
      601 cactgacgat atcgaactgc tctggtggat aaaaggagac gacgcatacg tcgaaaagcg
      661 ggacgagacg ggcgaatggc gtcccctgta cagcgagtgc cgcgcacggc cgtaacggtt
      721 ttgaactgac ggaagaagct ccctgcacgg tttgccctgg atcaatgcgc cgagttccgt
      781 gcacgggagc ttcctttctg gagacgaagc gtatgaacct gatgttgtca tgaccgcgtt
      841 cggtagtgtg gagtacccaa cttgctgctt cgcagcgcgg ttcaaagcgt cgcatccatc
      901 ttttcccgca gttcttccca gtttggaatc cccctgaaga gcagtttccc gtcgaaaacc
      961 cacgtcggtt ctgctcggat attaaaccgc accaggtcga aaaaccagaa aaggcatcgg
     1021 gggtcgtaga tatttgtctc gacgctcttg ccgtaatgcg ctcgaatctt gccgatcaga
     1081 tctcccaccg ccacacgggc ggttgcctgc gggaaggagg ccatctcctc gcttccgtac
     1141 agaaaaccgc ttcagcagac ggaggaaatc ggtttttcca tgtagatttt gaggcaatgc
     1201 cgcataggct tcactcccta tagtttgtcg gatacgagcg tatcgtattc caccgccctg
     1261 tactgttcgt acttcttccc atgaacgccg atggcggcag gagggcagaa agcgatgcat
     1321 cgctggcaga cgacacagtg atcaaggaaa cgcgggtact cctccatgac gatgtttttc
     1381 gcagggcaaa gacgggcgca ctttccgcac tgaatacacc tgctcttgtc gatttcgagc
     1441 gggaacgagc gtttcatcat tctccatggc gctttctttc gcgccatcca gttgaaaaac
     1501 ggcgaaaacg gtcccccgct gcaccatgtg gcgcgatctt cgagaagcgc gagcgcaaat
     1561 tcgcgagcct tcgcctcgca tttctggatt ttttccctgt tcgcttcctg cgggatactc
     1621 ttattcgcat agtttgacgg cataacgaat tccgcatacc cgagcgtcct gtaccccttt
     1681 ttgtcgagca gtttacgaag aggcgatcgc agcccccctg aataccctcc cattgtggag
     1741 agcgcgaatg cgct
//
LOCUS       DAOPKU010000625         1751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_80427_length_1751_cov_4.367925, whole genome shotgun sequence.
ACCESSION   DAOPKU010000625 DAOPKU010000000
VERSION     DAOPKU010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80427_length_1751_cov_4.367925"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PK849_14330"
     CDS             complement(<1..346)
                     /locus_tag="PK849_14330"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HPE67356.1"
                     /translation="MFLMLLFVLTLLLLLAASPSEAKTHSFFITKEQAYNGMLRSDIP
                     PALTIEDGDTVVFNTVMLMAGELSPEMTFDEMFALRTRMKERGLGVYAFTGPFFVKGA
                     EPGDVLEVRIKRI"
     gene            complement(534..950)
                     /locus_tag="PK849_14335"
     CDS             complement(534..950)
                     /locus_tag="PK849_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPE67357.1"
                     /translation="MSRTKIFIDTNILIYTVDAHDAEKQIRARSIVKSVVENDLPVIS
                     TQILQEFYSASTTKLKVDPLIAKNLVHNFGNMEVVPVDPHTIEQGIDISILCRISFWD
                     GLVVAAAEQGNCEILFSEDLNNGQTIRGIKILNPFF"
     gene            complement(943..1167)
                     /locus_tag="PK849_14340"
     CDS             complement(943..1167)
                     /locus_tag="PK849_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67358.1"
                     /translation="MRNITLSLDKETLRRGREYAKRHNISFNALVRRLVEQTVASSSS
                     QWLEDTFALMDQTPVSSQGEKWSREELHRV"
     gene            complement(1247..>1751)
                     /locus_tag="PK849_14345"
     CDS             complement(1247..>1751)
                     /locus_tag="PK849_14345"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPE67359.1"
                     /translation="LGDRVFVLRDGQTAESFPVERLWEKGARGEAWSALGWGNLFRGR
                     ITPCESDCACRFEGERVCLSLDGGAHSPGESLVFVAPDKIRILYPDIPVDPDLGGNVF
                     SAVVEETAPLGGSIRLYLASENGVLWQAEHPSESYQTLRLRPGSKVRFAVPPAGIESW
                     SGGPSPR"
BASE COUNT          384 a          477 c          520 g          370 t
ORIGIN      
        1 cgatgcgctt gatacggact tcgaggacgt ctcccggctc ggcccccttg acgaagaacg
       61 gcccggtgaa ggcgtacacg cccaggccgc gctctttcat gcgcgtgcga agggcgaaca
      121 tctcatcgaa cgtcatctcg ggcgacagct ctccggccat cagcatcacc gtgttgaaaa
      181 cgaccgtgtc gccgtcctct atcgtgagcg cgggggggat atcgctccgg agcatgccgt
      241 tgtacgcctg ttccttcgtg atgaagaagg agtgcgtctt cgcctcggag ggagaagcgg
      301 ccaggagcag cagaagcgtc agtacaaaaa gcagcattaa aaacatgcgt gttcctcctc
      361 gatgtgaaag tctgaagaaa gtatatcatc cggggagtga acgcgccgtc agcttctttt
      421 ctcgggtgag agggcgcgaa aattctcccg aacggagagc ggagaagttc gtacggcggc
      481 ttccgttcgg tacgcgacgt tgatgcaagc gggctttcgg gctgtgagaa aggttaaaaa
      541 aacgggttga ggatctttat tccccgtatt gtttggccat tattcaggtc ttccgaaaag
      601 agaatctcgc aattgccctg ctctgccgct gcaacgacaa gtccgtccca gaaggaaatc
      661 cgacagagaa tactgatgtc gataccttgt tcgatcgtgt gcggatcaac agggacaacc
      721 tccatgttgc cgaagttatg aacgaggttt ttggcaataa gaggatcaac cttcaacttg
      781 gttgtagagg cgctgtaaaa ctcctgcaaa atctgtgtgg aaatcacagg aagatcattc
      841 tccacaacgc ttttcactat ggaacgggcc cgaatctgtt tttcggcgtc gtgagcgtca
      901 accgtatata tcagaatgtt ggtgtcgatg aatattttgg ttctagacac gatgcagctc
      961 ttccctcgac catttctcac cttgcgacga gacgggcgtt tgatccatga gagcgaatgt
     1021 gtcttccagc cactgggaag aagaggacgc caccgtttgc tcgaccaaac gacgtacgag
     1081 cgcgttaaaa gaaatattat ggcgtttcgc gtactctctt ccccgtcgga gcgtctcttt
     1141 atcgagagaa agcgttatat tacgcatgat tccacctcct attcacacat aatacgtgcg
     1201 cactttcgtg ctgtcaagat atcgaatgga agagtatgca gttgcattat cgaggagaag
     1261 gccccccgct ccacgactcg atgcctgccg gagggacggc gaaacgcacc ttcgagcccg
     1321 ggcgaagccg gagggtctgg tacgactcgg aggggtgttc ggcctgccag aggacgccgt
     1381 tctccgaggc gagatagagg cggatgctcc cgcctagcgg cgcggtctcc tcgacgacgg
     1441 cggagaagac gttgccgccg aggtcggggt cgaccggaat gtcggggtag agaatgcgga
     1501 tcttgtcggg ggcgacgaag acgaggctct cgccaggcga atgcgctccc ccgtcgagcg
     1561 agaggcagac cctctctccc tcgaagcggc aggcgcagtc cgattcgcag ggggtaatcc
     1621 tgccacggaa gaggtttccc cagccgagcg cgctccacgc ctcgccgcgc gcgcctttct
     1681 cccagaggcg ttccacgggg aagctttcgg cggtttgccc gtcgcggagg acgaacaccc
     1741 tgtcgccgag c
//
LOCUS       DAOPKU010000626         1751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_80428_length_1751_cov_4.277712, whole genome shotgun sequence.
ACCESSION   DAOPKU010000626 DAOPKU010000000
VERSION     DAOPKU010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80428_length_1751_cov_4.277712"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PK849_14350"
     CDS             <1..276
                     /locus_tag="PK849_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67360.1"
                     /translation="AKAVLLAPTSRTPLDATGDMEKQNDPGSYLIQADSVSAPLRISL
                     PLPKSKEEEAKAVSAMLVEAAATMDLLEPFSRMGKALAALGEEHGEQ"
     gene            1014..1457
                     /locus_tag="PK849_14355"
     CDS             1014..1457
                     /locus_tag="PK849_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPE67361.1"
                     /translation="MADEKKGDVTPLAPGEVVVCVVEQIMPYGAFVRIKNGQRAMIHI
                     SELSHGFVKKVEDILALGQEVQARVIKIDEKGRIDLSLKKMEAPRPAASFGPRNAAAN
                     VPEDPRDSFEKKLSNYLKASEAKIADINSKLNSSKASKKRGKPAK"
     gene            1553..>1751
                     /locus_tag="PK849_14360"
     CDS             1553..>1751
                     /locus_tag="PK849_14360"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HPE67362.1"
                     /translation="MKGRVFDPSLVSAVVRRCPKGFPAVIQCVPLKNCKPFPTSFWLT
                     CPWLVRLCGMRESEGAVSVLEE"
BASE COUNT          400 a          437 c          511 g          403 t
ORIGIN      
        1 gccaaagcgg tgctgcttgc ccccaccagc agaactcccc ttgatgcaac cggggatatg
       61 gagaaacaga acgatcccgg ctcctacctg attcaggctg actcagtcag cgcgcctctc
      121 cgtatatcgc tcccccttcc caagtcgaaa gaggaagagg ccaaggcagt ttcggcgatg
      181 ctcgtcgagg ccgcggcgac aatggatctg ctcgaacctt tctccaggat gggaaaggcg
      241 cttgcagccc ttggtgagga acacggtgag cagtgaggat attcgcacgt atctggcaag
      301 gctctttctc ggaaaaacga aagtacactc tctccgtcct caagggaaag aggttgtgct
      361 tcggactacc gggagagtta tagagacgca tcctctccgc tctcccgaac gcgtacttgg
      421 ttccgccctg ttcggttctg cgatggatgt gctgactaag ggcttttgta tttcagaggc
      481 ggcgatcgga ggcattcggg acaatatccg cggcgggaag ttctctttcg acaggcgcct
      541 cgtggtgaaa ggcgaaagag ttgtgcgcga tacgcgggtt tttttgcact ccatcggtgg
      601 attcactgta cgttgtaaca aggctgtagg caggatctcg ccccttccga ttgaagtgcg
      661 gaatcggctt ccctggtttc gaggaaggtc gaattctctc aagaagggag aagttttgct
      721 tgcgctttac tgcccgatac tggaagacgg cgtgttgaaa agtgttctga acaaggagag
      781 aggaacgctt cttgtttggt ataatcctcg aagggcgtgg aaagcccgga gtttgtttct
      841 tgttcgggac ttctcctccg gggggagtct cgaatggaaa tggatcgata ttcactagcg
      901 cataatcgca gcctgcggag gtggttttca ccggaaacga aacagcggca cagcttgtcg
      961 atgcagtaag gactcaaagt atcaacaaat aatcgtggag ggtagacaac cgcatggcag
     1021 atgaaaaaaa gggagacgtc actcccctgg caccgggtga agtggttgtg tgcgttgtag
     1081 agcagattat gccctacggc gcttttgttc ggattaaaaa cggacagcgg gcgatgatcc
     1141 atatctccga gctgtcccac gggttcgtca agaaagtcga ggatatcctc gctcttggcc
     1201 aggaggtcca ggccagggta ataaagatcg acgagaaggg caggatcgat ctctccctaa
     1261 aaaaaatgga ggcgccaaga cctgctgctt cctttggtcc gcgaaacgct gcggcgaacg
     1321 ttcctgaaga tccccgggac tccttcgaga agaagctttc gaactatctc aaggccagcg
     1381 aggcgaagat cgccgatatc aacagcaaac tcaacagcag caaggcttca aagaaaagag
     1441 gcaaacctgc gaagtgaagc ggactctttc ttttttctcc cgggggcgga ttcgccccct
     1501 tctttttggg gagccgctga atggaaggga tagatctgtt ctgctgcgcc agatgaaagg
     1561 acgtgttttc gacccttcgc tggtttctgc tgttgtacgc cgctgtccga aggggttccc
     1621 tgcggtgata cagtgcgttc ctctcaaaaa ttgcaagccg ttccccacat cgttctggct
     1681 gacctgtcct tggcttgtca ggctttgcgg aatgcgagag tccgaaggag ccgtttccgt
     1741 tctggaagag a
//
LOCUS       DAOPKU010000627         1740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_81138_length_1740_cov_3.782196, whole genome shotgun sequence.
ACCESSION   DAOPKU010000627 DAOPKU010000000
VERSION     DAOPKU010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81138_length_1740_cov_3.782196"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1740
                     /locus_tag="PK849_14365"
     CDS             <1..>1740
                     /locus_tag="PK849_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPE67363.1"
                     /translation="GQNPASADRGTLARLKELQPDFDLVIVDEAHHLRNPGRKQWQAG
                     TTLSRNATAMVMLTATPVQLGRENLFHLLQILDKDEFPDNYGAEERFAINEKVVMAQN
                     CVAKIPPDVSKAAEYLDSAGYFEIIRKNPYYLQSRSALENLSRETGSAKSDEEWIRAQ
                     IAAQRSLASLNLISHIYTRTRRREVHEDFTRREAHPIQVEFTKYEEAFYKAVTHYVRA
                     HCAELGYSRGVEKWVLNMPQRQMASSIPAMVEHYMKTIPGVQRFPVVESEPADDLEAE
                     ESELRSENGENDFELERTQSFEEAQANLRDVLSQWPENATDSKYEAFVKALRKIRSGE
                     GSMKVLVFSFFKGTLRYLQRRLAEEGIRTVCIHGDVKSEERARLIAEFRDNPEIEVML
                     SSKVGSEGLDFQFCHILFNYDLPWNPMEVEQRIGRLDRIGQESPFIAIYHFWITGTIE
                     ERILKRLYDRIGVFERSIGELELILGDISRDLEQEIFSKDLTPHEETAALERKLLVLK
                     TREEELAKIESDAARFIGTDAFFEQEVEKTRKNRLYITPEQIKGLVEDFLRNHTPMTR
                     FDYNPEKETGRLFP"
BASE COUNT          427 a          487 c          519 g          307 t
ORIGIN      
        1 ggtcagaacc ccgcatcggc ggaccgggga acgctcgccc ggctgaaaga actgcaaccc
       61 gacttcgacc tggtcatcgt ggacgaagcg catcacctga ggaaccccgg aagaaagcag
      121 tggcaggcag ggacgacgct ctcccggaac gcgactgcca tggtgatgct gacggcgacg
      181 cccgttcagt tggggcggga gaaccttttt caccttctcc agatcctcga caaggacgag
      241 tttcccgaca attacggggc ggaggagcgc ttcgcgatca acgagaaggt cgtcatggcg
      301 caaaactgcg tcgcgaagat acctcccgac gtctcgaaag ccgctgagta cctggattcg
      361 gcaggatact tcgagatcat ccgcaagaac ccctactatc tccagtcgcg gagcgctctg
      421 gagaatctgt cccgcgaaac ggggagcgcg aagagcgacg aggagtggat aagagcgcag
      481 atagccgctc agcgaagcct cgcctcgctc aacctcatca gccacatcta cacccgaacg
      541 cgaagaagag aagttcacga ggatttcacc cgcagagagg cgcacccgat tcaggtggag
      601 ttcaccaagt atgaggaagc cttctacaaa gcggtcacgc actatgtccg agcccactgc
      661 gccgaactcg gatactcccg gggggttgaa aagtgggttc tcaacatgcc gcagcggcag
      721 atggcatctt ccattccggc gatggtggag cactatatga aaacgattcc gggcgttcag
      781 cgattccccg tcgtcgaaag cgagccggcc gacgacctgg aagcggaaga gtcggaactg
      841 cgttcggaaa acggggagaa tgacttcgaa ctcgaacgga cgcagtcgtt cgaagaggcg
      901 caggcaaatc tgcgggacgt gctgtcgcag tggcccgaga acgcgacgga ttcgaaatac
      961 gaagcgttcg taaaggcgct tcgcaaaata cgatccggag aaggaagtat gaaagtcctc
     1021 gtcttctcct tcttcaaggg gacgcttcgc tacctgcaaa ggcgcctcgc cgaggaggga
     1081 atccgtaccg tctgcatcca cggcgacgtg aagagcgagg aacgcgcccg tctgatcgcc
     1141 gaattccggg acaaccccga gatcgaggtg atgctctcct cgaaagtcgg ctcggaggga
     1201 ctcgactttc agttctgcca catccttttc aactacgacc ttccctggaa ccccatggag
     1261 gtggagcagc gcattggccg tctggaccgg atcggacagg agtccccgtt catcgctatc
     1321 tatcacttct ggatcaccgg aacgatcgag gagcgaatcc tcaagcgtct ttacgatcgt
     1381 atcggcgtct tcgagcgttc tataggagag ctggagctga ttcttggcga catatcgaga
     1441 gatctggagc aggagatctt ctccaaggat ctgacgcctc acgaggagac cgcggcgctc
     1501 gaaaggaagc tgctggtgct gaagacaaga gaggaggagc ttgcgaagat cgagtcggat
     1561 gcggctcgct tcataggaac cgacgccttt ttcgagcagg aggtagaaaa gacgagaaag
     1621 aaccggctgt acatcacgcc agagcagata aaagggctgg tggaggactt tcttcgaaac
     1681 cacactccga tgacgcgctt cgactacaat cccgaaaaag agaccggacg cttatttccc
//
LOCUS       DAOPKU010000628         1735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_81447_length_1735_cov_5.743452, whole genome shotgun sequence.
ACCESSION   DAOPKU010000628 DAOPKU010000000
VERSION     DAOPKU010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81447_length_1735_cov_5.743452"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PK849_14370"
     CDS             complement(<1..331)
                     /locus_tag="PK849_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67364.1"
                     /translation="MTEQPTTVQKENGGRSGKRWFLPFLTIFAVLAYLSFRASFSLIG
                     PLAWSALISFTVYPLYIRMRRRFFSRRSANLPALLATAIIVFAVLIPCLLGLFFAARE
                     GVRLLDST"
     gene            435..>1735
                     /locus_tag="PK849_14375"
     CDS             435..>1735
                     /locus_tag="PK849_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPE67365.1"
                     /translation="MILRGCASLRLFSTLFLALLLWSGGAYAAGFPPAMIRDASRAVK
                     MLVTEGNGDRMGQGTGFFVTPDGLMLTNYHVVEGNRTIKIWDEKNLYDIVRIVAFDKN
                     ADVALLETNYPKSSVRHLPLRKDVVELGEPIMVLGYPRAFQLGDSITVTKGDISSIRK
                     VGDDTLVQFTAFVAGGSSGSPIIDEKGRVAGIVTSELTLGQGMFLGLSAPSIQRYVGE
                     HLPKVEELIVDLPPPPPVIRSPALPAAPRELPPDTSAMVKEAQELLNALGYDAGAEDG
                     VYGPRTSAAVKAFQRVKKLRRDGHVTKKLLDLLREEKRKTDETRAAAIGREIQSLTEN
                     AMRGNPDAQRELGLLYREGRRVPPDYSKAAGWLRMAALHGDVVAQTALASMYRYGLGV
                     PKDPVQSASWMTAAAELGHAPAQLALGEMFATAFGVPLDRKR"
BASE COUNT          322 a          535 c          595 g          283 t
ORIGIN      
        1 ccgtgctgtc gagcaggcgc accccctcgc gcgcggcgaa gaaaagcccc agcaggcagg
       61 ggatcaggac ggcgaagacg atgatcgcgg tggccagaag ggcgggcagg ttcgcggaac
      121 ggcgggagaa aaagcgccgc cgcattcgta tgtacagcgg atagactgtg aaggagatga
      181 gcgcggacca ggcaagaggt cctatcaggg agaaggaagc ccggaaggag agatacgcga
      241 gcaccgcgaa gatcgtcaga aagggaagaa accatcgttt cccgcttcgt ccgccgtttt
      301 ctttttgaac tgttgtcggc tgttccgtca tgtacgccac ccgctttcgt ccgattgtgg
      361 tattctcttt tgagtaagga taccactcgg cgcggcgaat cgcctccgcc gttcgacgat
      421 tcggggtgat gtgtatgatt ttacgaggct gcgcctcgtt gcggcttttt tcgacgctct
      481 ttctcgcgct ccttctgtgg agcgggggcg cgtacgcggc gggattcccg cccgcgatga
      541 tccgcgacgc ctcccgcgcc gtcaagatgc tggtgacgga gggcaacggc gaccgcatgg
      601 ggcaggggac gggctttttc gtcacgcccg acggtctgat gctgacgaac tatcacgtgg
      661 tcgaggggaa caggacgatc aagatatggg acgagaagaa cctctacgac atcgtccgga
      721 tcgtggcgtt cgacaagaac gccgacgtcg ccctgctgga gacgaactat ccgaagtcgt
      781 cggtgcgcca cctcccgctg aggaaggacg tcgtcgaact cggggagccg atcatggtcc
      841 tcgggtaccc gcgcgccttc cagctgggag actccatcac cgtgacgaag ggggacatca
      901 gctccatccg gaaggtcggc gacgacacgc ttgtgcagtt caccgccttc gtggcggggg
      961 gctcgagcgg cagccccatc atcgacgaaa aggggcgtgt cgccggaatc gtgaccagcg
     1021 agctgaccct cggacagggg atgttcctgg ggctgagcgc gccgtcgata caacggtatg
     1081 tcggcgagca tctgccgaag gtcgaggagc tgatcgtcga tctgcctccc ccgccgcccg
     1141 tcatccggtc gcccgcgctc cctgcggccc cgcgcgagct tcctccggac acctcggcga
     1201 tggtgaagga ggcgcaggag cttctgaacg cgctcggcta cgacgcggga gctgaggacg
     1261 gcgtgtacgg cccgcggaca tccgccgccg taaaggcgtt ccagcgggtg aaaaagctcc
     1321 gccgcgacgg ccacgtgacg aagaagctgc tcgacctcct gcgggaggag aaacgaaaga
     1381 ccgacgagac gcgggccgcc gccatcggga gagagattca atctctcacg gagaacgcga
     1441 tgcggggaaa tccggacgcg cagcgcgagc tcggcctgct ctaccgcgag gggcggcgcg
     1501 ttccgcccga ctattcgaaa gccgccgggt ggctgcgcat ggctgcgctg cacggcgacg
     1561 tcgtcgccca gaccgcgctt gcctccatgt accggtacgg cctcggcgta ccgaaagatc
     1621 ctgtgcagtc cgcctcctgg atgaccgccg ccgcggagct ggggcacgcg cccgcgcagc
     1681 tcgcgctggg ggagatgttc gcgaccgcct tcggcgtgcc gctggacaga aaacg
//
LOCUS       DAOPKU010000629         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_81852_length_1730_cov_3.331940, whole genome shotgun sequence.
ACCESSION   DAOPKU010000629 DAOPKU010000000
VERSION     DAOPKU010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81852_length_1730_cov_3.331940"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /gene="hcp"
                     /locus_tag="PK849_14380"
                     /gene_synonym="priS"
     CDS             <1..775
                     /gene="hcp"
                     /locus_tag="PK849_14380"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPE67366.1"
                     /translation="EEFESFNGPVFLTTNCLVPLKKENTYLDRLFTTGVASYPGAKHI
                     PDRQKGGAKDFSAMIEMAKKCAAPKEIETGKLVGGFAHNQVMALADKVVAAVKSGAIK
                     RFVVMAGCDGRFKSRDYYTDVAKNLPKDTVILTAGCAKYRYNKLDLGDIGGIPRVLDA
                     GQCNDSYSLAVIALKLKEAFGLDDINKLPISYDIAWYEQKAVAVLLALLSLGVKGIRL
                     GPTLPGFLSPNVAGVLVKNFDIKPISTVEEDVAAMMAGK"
     gene            complement(880..1329)
                     /locus_tag="PK849_14385"
     CDS             complement(880..1329)
                     /locus_tag="PK849_14385"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HPE67367.1"
                     /translation="MSSDIVRIGIFICNRYHTCAGGKCLRSIRNREGAFSIYKDREVE
                     LVGYTTCDGCPGGNIEYAPAEMKKNGATVIHLATGMVVGYPPCPRIDYFRRYIKTAFD
                     LPVVVGTHPIPEKYFVIHEALGTWNSPMWQELIAPTLATPEIRLAYN"
BASE COUNT          364 a          512 c          513 g          341 t
ORIGIN      
        1 cgaggagttc gagtccttca acggacccgt cttccttacc accaactgcc tggttccgct
       61 gaagaaggaa aacacctacc tcgacaggct gttcaccacc ggcgtcgcca gctaccccgg
      121 cgcgaagcac attccggaca gacagaaggg cggcgcgaag gacttctccg cgatgatcga
      181 gatggcgaag aagtgcgccg cgcccaaaga gatcgagacg ggcaagctcg tcggcggatt
      241 tgcgcacaac caggtcatgg cgctcgccga caaggtcgtc gcggccgtga agtccggcgc
      301 gatcaagcgc ttcgtcgtca tggcggggtg cgacggccgc ttcaagtcac gcgactacta
      361 caccgacgtg gcgaagaacc tgccgaagga caccgtcatt ctcaccgcgg gctgcgccaa
      421 gtaccgctac aacaagctcg acctcggcga catcggcgga atccccaggg tgctcgacgc
      481 cggacagtgc aacgactcct actcgctggc cgtgatcgcc ctgaagctga aggaggcgtt
      541 cggcctcgac gacatcaaca agctgcccat ctcctacgac atcgcctggt acgagcagaa
      601 ggcggtcgcg gtccttctgg ccctcctctc gctcggcgtg aagggcatcc gcctcgggcc
      661 cacccttccc ggattcctct ctcccaacgt cgcgggagtg ctggtgaaga acttcgacat
      721 caagcccatc tcgacggtgg aggaagacgt cgccgcgatg atggcgggca agtagtcggc
      781 gcggcgcaag cgcgccaaaa atgaaaagag cgggaggatc gcccttcgaa aaggcgtgat
      841 cctcccgctt ttttgtgatg aaatcgtttc gcccggcctc tagttgtacg ccaggcggat
      901 ctccggggtg gcgagcgtcg gggcgatcag ctcctgccac atcggagagt tccacgtccc
      961 cagcgcctcg tggatgacga agtacttctc cgggataggg tgcgtgccca cgaccacagg
     1021 aagatcgaat gccgttttga tatagcggcg gaagtagtct atccggggac agggcgggta
     1081 gcccacaacc atgcccgttg cgaggtggat caccgtcgcg ccgttcttct tcatctcggc
     1141 gggggcgtac tcgatgttgc cgccgggaca gccgtcgcag gtagtgtagc cgacgagttc
     1201 gacctctctg tccttgtaga tactgaacgc accctctctg ttgcgtatgg agcgcaggca
     1261 cttgcccccc gcgcacgtgt ggtagcggtt gcagatgaaa atacctatcc ggacgatgtc
     1321 gctgctcatg aaaacgcctc ctcgggtcga aattccggcc gtacggccag cttgttgata
     1381 tctgttctca ttactataaa cctcccccgc cgatttgcaa ccccgagcat ttcaaaaaga
     1441 gagtgcgtcc ttcggcggac gcagcaggaa caatggaatt tgccggagga atctcgtgga
     1501 cgcggagaga cgctggtaaa gagatcccga ggtctgttcg tggaaaagcc cgcggaactt
     1561 gtgtgcccgc agaaggtttt tttgaggaac agggaaagct tcctgcgcct gtcggggaaa
     1621 aatcttcctt gcgctaatct ggaatacgaa ttataatctc actctgagga attcaattta
     1681 ttccgtattt gattcgaaag ggtgagtgtt cttcatgaag ggtttcaaca
//
LOCUS       DAOPKU010000630         1727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_82060_length_1727_cov_3.647129, whole genome shotgun sequence.
ACCESSION   DAOPKU010000630 DAOPKU010000000
VERSION     DAOPKU010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82060_length_1727_cov_3.647129"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PK849_14390"
     CDS             complement(<1..433)
                     /locus_tag="PK849_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPE67368.1"
                     /translation="MTQDVQREQWGSKIGFILAAAGSAVGLGNIWRFPYVTGQNGGAA
                     FVLIYLAIVFIIGFSVMLAEMAIGRKAQLNAVGSFSKLAGGLWPLVGWMGVAAGFIIL
                     SFYAVIGGWTIKYFIHSFTGLMADAAAGKAGDVFGGFVTNTM"
     gene            645..905
                     /locus_tag="PK849_14395"
     CDS             645..905
                     /locus_tag="PK849_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPE67369.1"
                     /translation="MCLAVPHVIQEILDGKKAVALAGAVRTEIRTDLVDSLSCGDTVL
                     VHAGFAIEKLSPADSEELLALWEEVRSLAGETLRGSLTHDAV"
     gene            895..>1727
                     /gene="hypD"
                     /locus_tag="PK849_14400"
     CDS             895..>1727
                     /gene="hypD"
                     /locus_tag="PK849_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPE67370.1"
                     /translation="MLSNVAAASEALKTLVAAPISVMEVCGTHTVSIFRTGLRSLLPQ
                     SLRLVSGPGCPVCVTDQGEIDCALSLLETGAIVAAYGDMFRVPGKSGSLASKRSAGFD
                     ARLVTSASDVLAIAENEPRNEVVFLAVGFETTAPATAALVLEASERNIANLSILSFHK
                     RTPPALKALSADPALAISGFLLPGHVCVILGHEPFRFLAEQHGIPAVIAGFDPEQIML
                     GLVAIALQKKQGEATLRSVYGQAVRPEGNTKARELMESVFISKDASWRGLGILPDSGL
                     V"
BASE COUNT          377 a          507 c          445 g          398 t
ORIGIN      
        1 gcatcgtatt cgtgacgaac ccgccgaaca catctcctgc tttcccggcc gcagcatcag
       61 ccatgagccc ggtgaaagag tggatgaagt atttgatcgt ccagccgccg atgaccgcgt
      121 agaaagaaag aatgataaat cctgcggcga cacccatcca accgacaaga ggccagaggc
      181 caccagccag tttactgaac gaaccgacgg cattcagctg cgcctttctg ccgattgcca
      241 tctccgcgag catcacggag aaaccgataa taaacacgat agcgaggtag atgaggacga
      301 aagccgcccc accgttctgt cccgttacat aagggaacct ccagatgttg ccaagtccta
      361 cagccgatcc cgccgccgcc agaataaatc ctatcttgct accccattgc tctctctgca
      421 catcctgtgt cattataccc atacccctcc ttcatcatcg atttgagcgt atacacatcc
      481 tgacacaacg acaatctctc cttataccca cccccctatt ccaatgatat tcacaatata
      541 taataatcgc attgtattat acaccagatc aggattcttc caagtggggt gagctgtttt
      601 tcctttttct ggagtacaat agagcaagaa aaggagtgat caccatgtgt cttgctgttc
      661 ctcacgtcat tcaggaaatc ctggacggga aaaaagcggt tgctcttgcc ggagccgtcc
      721 ggacggagat acgaaccgat ctcgtagatt ctctctcttg cggagatact gtgcttgtcc
      781 acgcggggtt tgctatagaa aagctctctc ctgcggattc ggaagaactg ctcgcacttt
      841 gggaggaggt ccggagtctc gccggagaga ctctcagggg tagcctgacg cacgatgctg
      901 tctaacgtag ccgcggcctc ggaggcgctg aaaacgctcg tcgccgcgcc gatctcggtg
      961 atggaggtat gcggcacaca tacggtttcc atcttccgca ccggacttcg cagtctgctt
     1021 cctcagtcgc ttcgccttgt ttcggggccg ggatgccccg tatgcgtaac tgaccagggg
     1081 gagattgact gcgcgctctc cctcttggag acgggcgcga ttgtcgccgc gtacggcgat
     1141 atgttccgcg tccccggtaa atcaggttct ctcgcgtcga aacggagcgc cggtttcgac
     1201 gcgcgattgg tgacaagcgc ctccgacgtt cttgcgatcg cagaaaatga accccggaac
     1261 gaggtggtct ttctcgccgt cggctttgaa accactgcgc ccgcgacagc cgcgcttgta
     1321 cttgaagcct ccgagaggaa catcgccaat ctttcgattc tttcgtttca taagcgaacg
     1381 cctcccgccc tcaaagcact ctctgccgat cccgctcttg cgatctccgg ttttcttctt
     1441 ccgggacatg tttgcgtcat cctcggccac gaaccgtttc gcttcctggc ggagcagcat
     1501 gggattccgg cagtgattgc agggttcgac cctgaacaga ttatgctcgg gctcgtagcg
     1561 atcgcgcttc agaaaaaaca gggtgaggcg actcttcgct cggtctacgg acaggcggta
     1621 cgccccgaag ggaacacaaa agcgcgggag ctcatggaga gtgtttttat cagcaaagac
     1681 gcttcgtgga gaggacttgg aattcttccc gattccggac ttgtttt
//
LOCUS       DAOPKU010000631         1726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_82132_length_1726_cov_3.366846, whole genome shotgun sequence.
ACCESSION   DAOPKU010000631 DAOPKU010000000
VERSION     DAOPKU010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82132_length_1726_cov_3.366846"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PK849_14405"
     CDS             <1..1322
                     /locus_tag="PK849_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPE67371.1"
                     /translation="GHEDFSEDTYRVLTAVDSVIMVIDSVKGVEAQTRKLMEVCRMRN
                     TPIITFINKLDREGMAPLDILGDIEEKLQVECAPLSWPVGMGKRFKGTYNLYKKELNL
                     FTPGSDRVEEEVRVIRDVNDPALDAALGSQADELREDLALLEGAANPFEMEHYLRANQ
                     TPVFFGSAINNFGVKELLDTFVEIAPSPLPRPAVSRTVAPEEEAFSGFVFKIQANMDP
                     SHRDRLAFMRVCSGKFERGMKMRHHRIGKDVTVANPVIFMAQERALAEEAWPGDIIGI
                     HNHGTIKIGDTFTDKEPLKFTGIPSFAPEHFRRVRLQSPLKAKQLQKGLLQLTEEGAV
                     QLFRPLGRNDYILGAVGVLQFDVTMARLKDEYGVEATYEPVNYETARWVTCADEKRLA
                     EFQKANQNDLAIDGEGNLAFLADGEWRLRHVSEKWPGIEFHTTRELR"
     gene            1346..>1726
                     /locus_tag="PK849_14410"
     CDS             1346..>1726
                     /locus_tag="PK849_14410"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPE67372.1"
                     /translation="MEKTILDLAGELLAKSEVVTLASVTEEGFPRVCAMARVRSEGVK
                     TVWMATGTYSKKTAHFRKNPKASVCAYSGGDSLTLLGNVAVLTDEQTRKDLWQEWFIK
                     HFPKGPDDPDYCVLKFDAVEATVYI"
BASE COUNT          332 a          500 c          632 g          262 t
ORIGIN      
        1 cgggacacga ggatttctcg gaggacacct accgcgtgct caccgcggtg gacagcgtga
       61 tcatggtcat cgacagcgtg aagggcgtgg aggcgcagac gcgcaagctg atggaggtct
      121 gccggatgcg caacacgccg atcatcacct tcatcaacaa gctcgaccgc gaggggatgg
      181 cgccgctgga catcctgggc gacatcgagg agaagctcca ggtggagtgc gcgcccctgt
      241 cgtggcccgt cgggatgggg aagcgcttca aggggacgta caacctgtac aaaaaagaat
      301 tgaacctctt cacgccgggg agcgaccggg tggaggagga agtccgcgtg atcagggacg
      361 tgaacgaccc cgcgctcgac gcggcgctgg ggagccaggc cgacgagctg cgcgaggatc
      421 tcgccctgct cgagggggcg gcgaacccgt tcgagatgga gcactacctg cgggcgaacc
      481 agacgccggt tttcttcggc agcgcgatca acaacttcgg cgtgaaggag ctgctggaca
      541 cgttcgtgga gatcgccccg tcccccctgc cgcgccccgc cgtttcgcga accgtcgcgc
      601 cggaggagga ggcgttctcc ggcttcgtct tcaagattca ggcgaacatg gacccgtcgc
      661 accgcgaccg gctggccttc atgcgcgtct gctcgggcaa gttcgagcgg gggatgaaga
      721 tgcggcacca ccgcatcggc aaggatgtga cggtggccaa tccggtgatc ttcatggcgc
      781 aggagcgcgc gctggcggaa gaggcgtggc cgggcgacat catcggcatc cacaaccacg
      841 gcacgatcaa gatcggcgac acgttcacgg acaaggagcc gctgaagttc acgggcattc
      901 ccagcttcgc gccggagcac ttccggcggg tgcgcctcca gtcgccgctg aaggcgaagc
      961 agctccagaa ggggctgttg cagctgacgg aggagggggc ggtgcagctc ttccggcctc
     1021 tggggcgcaa cgactacatc ctgggcgcgg tgggcgtgct gcagttcgac gtgacgatgg
     1081 cgcggctgaa ggacgagtac ggcgtggagg cgacctacga gccggtgaac tacgagacgg
     1141 cgcgctgggt gacctgcgcg gacgagaagc gccttgcgga gttccagaag gcgaaccaga
     1201 acgacctcgc catcgacggc gaggggaacc tggccttcct ggcggacggc gagtggcggc
     1261 tgcgccacgt ctccgagaag tggccgggga tcgagttcca caccacgcgc gagctgcggt
     1321 gagggatata aaggaggggg ccacgatgga gaagacgatt cttgatcttg ccggggagct
     1381 gctggcgaag tcggaggtgg tgacgctggc ctcggttacg gaggaggggt ttccccgcgt
     1441 ctgcgcgatg gcgagggtgc gctccgaggg cgtcaagacg gtgtggatgg ccaccggcac
     1501 gtattcgaaa aagacggcgc acttccggaa gaacccgaag gcgagcgtct gcgcgtattc
     1561 gggcggcgac agcctgacgc tgctggggaa cgtcgccgtg ctgacggacg agcagacgag
     1621 gaaagatctg tggcaggagt ggttcatcaa gcacttcccg aaggggccgg acgatcccga
     1681 ctattgcgtc ctgaagttcg acgccgtcga ggcgaccgtc tatatc
//
LOCUS       DAOPKU010000632         1722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_82435_length_1722_cov_3.195561, whole genome shotgun sequence.
ACCESSION   DAOPKU010000632 DAOPKU010000000
VERSION     DAOPKU010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82435_length_1722_cov_3.195561"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PK849_14415"
     CDS             <1..328
                     /locus_tag="PK849_14415"
                     /inference="COORDINATES: protein motif:HMM:NF019477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif11 family protein"
                     /protein_id="HPE67373.1"
                     /translation="PRQKRGYAMMAEKNIEDFFEEVRASTDLQDSLEALQESCGNDLL
                     KMYVALAGEKGYDFSETQLQAALEEKAGEMIPEGPLDDEQMESVAAAGCPYCFFTRGS
                     YCFFTK"
     gene            352..>1722
                     /locus_tag="PK849_14420"
     CDS             352..>1722
                     /locus_tag="PK849_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HPE67374.1"
                     /translation="MIRTIAAGACFLREKSAYCSAVASEIATPEPWNPERRELLERWI
                     RHAGGNGGKSAFLTRLAAEGIEERTLQCLLSSENGAGFEEPLPEWAVRLQKILEPSSS
                     EEEGASIPADSEPLFTALKPFLLFAARALREHLGKEREDTCLPASVHDELLAGLAKRL
                     MRIGEKTYRAEIHRRLAVAYPLQIILGRPPAQEITTTEAKRLAADLRTGGWFDLLRTY
                     PVLGRRLSETTGDWVTYTVELLDRLKKDCYELEQVFSISPSIAALSGMETDASDPHNG
                     GRAVAVLRWKTGERLVYKPRSGGIDQAWRRWIAWVESAGLPCCPVPAALDKGDYCWVE
                     YLTPRPLSDKEDAELFYHRSGVLLCIFYVLGANDFHMENMIAQGSYPVPVDLETLLVH
                     RFQPFVQEDEGTGAAREALRMLVDSVLHIGLLPIWVTDGRGNAEDISGLTGFAPRGSN
                     LPVLEGR"
BASE COUNT          357 a          471 c          540 g          354 t
ORIGIN      
        1 tccacgacaa aaaagggggt acgcaatgat ggccgagaag aatatcgagg atttttttga
       61 agaggtgcgc gcttccacgg accttcagga ttccttggaa gccctgcagg agagttgcgg
      121 caatgacctg ctgaagatgt acgtcgcgct tgcgggggaa aaaggctatg atttctccga
      181 gacgcagctc caggcggctt tggaggaaaa agcgggggag atgatcccgg aaggcccctt
      241 ggacgacgaa cagatggagt ccgttgccgc ggcagggtgc ccgtactgct tttttacccg
      301 cggcagctac tgcttcttca ccaagtgaca accgtacacg aaacgggaaa tgtgatccgc
      361 acgatagcgg cgggagcgtg tttcctgcgc gaaaaatcgg cgtattgcag cgctgtcgcc
      421 tctgaaatcg caacgcccga gccatggaat cccgagcgcc gtgaactgct cgaacgatgg
      481 attcggcatg cgggaggaaa cggcggcaaa agcgcctttc tcactcgtct tgcggcggaa
      541 ggcattgaag agcgaacgct tcaatgcctt ctttcttcgg agaacggcgc cggattcgaa
      601 gaaccgctcc ccgaatgggc ggtccggctg cagaaaatcc tcgagccgtc gtcttcggaa
      661 gaggagggcg cttcgattcc tgccgattcc gagcctcttt tcacggcctt gaagcccttt
      721 cttctgtttg cagcgcgcgc cctacgggag catctcggga aagaaaggga ggatacctgt
      781 ctccccgctt ccgtgcacga cgaactcttg gccggattgg cgaaacgatt gatgcggatc
      841 ggcgagaaaa cctatcgcgc cgaaatccac cgcagacttg cagttgccta cccgttgcag
      901 atcattctcg gccgtccgcc ggcgcaggag attaccacaa ccgaagccaa gcggctggcg
      961 gccgacctgc gaacgggggg ctggttcgat ctgctccgga cttatccggt gctcggccga
     1021 cgtctttccg agacaacggg agattgggtg acatatacgg tcgaactcct ggatcgattg
     1081 aaaaaagatt gctacgagct ggagcaggtc ttcagcattt ccccgtcgat cgccgcactg
     1141 agcgggatgg agacggatgc gtccgaccct cacaacggag gccgggccgt ggcagttctt
     1201 cgctggaaga cgggagagcg tctggtctac aaaccgcgaa gcggaggaat agatcaggct
     1261 tggcgtcgct ggatcgcatg ggtcgagtcg gcggggttac cgtgctgtcc ggttccggcg
     1321 gcgctggaca aaggcgatta ctgctgggtc gaatatctta ctccgcgccc cctgtcggac
     1381 aaggaggacg ccgaactctt ttatcatcga tccggagttt tattgtgcat attctacgta
     1441 ttgggagcca acgacttcca tatggagaac atgatcgccc aaggttcgta ccctgtgccg
     1501 gtggatctgg aaaccctcct cgtccaccgc tttcaacctt tcgttcagga ggacgagggg
     1561 acgggcgcgg cccgggaggc tctacgtatg cttgtcgact cggtgcttca tatcggtctt
     1621 ctgccgatat gggttaccga cggccgcgga aatgcggagg atatcagcgg tttgacgggg
     1681 ttcgctccga gggggagcaa tcttcccgtc ctggaagggc gt
//
LOCUS       DAOPKU010000633         1721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_82474_length_1721_cov_4.579232, whole genome shotgun sequence.
ACCESSION   DAOPKU010000633 DAOPKU010000000
VERSION     DAOPKU010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82474_length_1721_cov_4.579232"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1721
                     /locus_tag="PK849_14425"
     CDS             21..>1721
                     /locus_tag="PK849_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPE67375.1"
                     /translation="MRLREAEVRLARVLEASKISVWDYFPDKDATFATGRAILYGDAE
                     NEALLVPRKALWDKLVPPQLRPLLEEKLRQLLSGTVDSAEQEIDIPGPGDTKRRAVSK
                     AFPVRDAQGNVVRIAGLNIDVTKLKETERRIARQNKRLELFHATFLVLMEETDTEKLL
                     GRILEKATELARTEHGRISLLDTEKNVFRLVLGRGFLKAMEGETRPSDAGLAGEVLRQ
                     KKCVVIKEYPSFYGKIDDPRLARLTTVIGMPLFQGDVFFGVLSVAYWDVFPEVDEEIL
                     EDLDQFAGAVSIALENARLYESARRELKERVFAGERLRFHARLVDAAARASGVLLSEE
                     KREDALFCALYLLAAAANACEAALFRNSEGPDGTILANVVGRSTPEEAEEDSRRPPSS
                     LVLRDMSPVLLSALEKGIPFHGTLEGAEEPFSSMCGASACSRSSLDSVVIPVHLRGQF
                     WGFLALCFRERRSAFEGDELGVLRSFACTLAASVSRWESELEVSAGYENLQKTFSDVI
                     RTMGQIVGKKDPYTIEHQERVGTLASAIGARMNLDAERCDGLRIAGLVHDVGKIEIAG
                     E"
BASE COUNT          349 a          458 c          544 g          370 t
ORIGIN      
        1 cagggagaaa actgtcggag atgcgcctca gggaggccga ggtccgtttg gcccgcgtcc
       61 tggaggcctc gaaaatcagc gtgtgggact acttccccga caaggacgcg acgttcgcaa
      121 ccggacgggc tattctctac ggggacgcag aaaacgaggc gcttctggtt cccagaaagg
      181 cgctttggga caagctcgtt cctccgcaac ttcgccccct gctcgaggaa aaactgcgcc
      241 agttgctctc aggaacagta gattccgccg agcaagagat tgatatcccg ggtcccggcg
      301 atacgaagcg ccgcgcagtt tcgaaggcgt ttccggttcg ggacgcgcaa ggaaacgttg
      361 ttcgtattgc cggtttgaat atcgacgtga ccaaattgaa ggagaccgaa cggaggatcg
      421 ctcgtcagaa caaacgtctt gagctgtttc acgccacgtt ccttgtgctc atggaagaga
      481 cggatacgga aaaactgctc ggacgtattc tggagaaggc cacggagctc gcgcgtacgg
      541 aacatggaag gatctctctc ctggatactg aaaaaaatgt cttccggctt gttctggggc
      601 gaggatttct gaaggccatg gagggggaga cccggccgag cgacgccgga cttgcaggag
      661 aggtgctgcg gcagaaaaaa tgtgtggtta tcaaagagta tccatccttt tacggcaaga
      721 tagacgatcc tcgccttgcg aggctcacca cagtgatagg aatgcctctc ttccaggggg
      781 atgttttttt cggcgtgctc tcggtcgcct attgggatgt gtttcccgag gtagacgaag
      841 agatccttga ggacctcgat caattcgccg gggccgtctc gatcgcactg gagaatgccc
      901 gcctctatga gagcgcccgg cgggagctga aggagcgggt gttcgctgga gaacggcttc
      961 gctttcatgc tcgtctcgta gatgccgcag cccgggcctc cggcgtgctg ctctccgagg
     1021 aaaaaagaga agacgcgctc ttctgcgcgc tctatctgct cgccgcggct gcaaatgcct
     1081 gtgaagccgc gctgttccgc aacagtgagg gtcctgacgg gacgatactc gcgaatgttg
     1141 ttggccgttc tactcctgaa gaggccgaag aagactcgcg aagaccgcct tcatccctcg
     1201 ttttaaggga catgtcgccc gtacttctct ctgctctgga aaaaggcatt ccgttccatg
     1261 ggactcttga aggcgccgaa gagccttttt cttcgatgtg cggcgcttcc gcctgctcgc
     1321 gttcttcgct cgacagcgtg gtgatccctg tgcacctgcg cggtcagttc tgggggttcc
     1381 tcgcgctgtg cttccgggaa cggcgttccg ctttcgaagg tgatgaactc ggtgtgctgc
     1441 ggagttttgc gtgcaccctg gccgcctcgg tgagccgctg ggagtccgaa ttggaggtga
     1501 gcgccgggta cgagaacctg cagaagacgt tttcggatgt catcaggacg atgggacaga
     1561 ttgttggaaa aaaagacccg tacacaatag agcaccagga gcgcgtcggg acgctggctt
     1621 cggcgatcgg cgccaggatg aacctcgatg cggagcgctg cgatggtctt cgtattgcag
     1681 gtctcgtgca tgacgtcggc aagattgaga tagccggtga g
//
LOCUS       DAOPKU010000634         1720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_82550_length_1720_cov_3.860661, whole genome shotgun sequence.
ACCESSION   DAOPKU010000634 DAOPKU010000000
VERSION     DAOPKU010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82550_length_1720_cov_3.860661"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PK849_14430"
     CDS             complement(<1..1082)
                     /locus_tag="PK849_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPE67376.1"
                     /translation="MRIRSINAEKMGLSNRLTLLNLIRTTPGVSRRELARLAGLDPST
                     VTKIITSLLEKGLVIEHGVRDADHPGRKSIMLRVYRDAAVALIAEIGVEKTRIAKGFL
                     DASFEVLDEFDTPPKEDEFFSVFAGSLRKKAFSFPHTAYCALSVAVPGMVEWGSNVLR
                     SLPHMGWKNVDFEARLGKLLPEMELTVFTANEAKLSLIAEMFRNGGVSGLHSGVYVYL
                     SQGVGGAILMGDKIIHGLSNTAGEIGHMTVDPSGPLCHCGNRGCFETFVSIDTVVRNF
                     ENLGASLPGSDFREKFRALLRLFQEGNDAARRVLEEMGEFLATGIANLVSILNPDFIM
                     LGGMGSILPSSFVADVGDAVAKKTPVA"
     gene            complement(1207..>1720)
                     /locus_tag="PK849_14435"
     CDS             complement(1207..>1720)
                     /locus_tag="PK849_14435"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPE67377.1"
                     /translation="TWHGPRRNLHSIRAFEVRDAAGPVASAWTSWILIDTLRGRPLRL
                     DRHTTEVYTREAYPMDGDAPSLPVVEDFAYDTFFSVRRWDLDRNAHVNNAVYFSWAVE
                     SAPDAVAAEYELVKVEAEYLKPVEKNGDVRVRTALLPADQGEKAFFHLIEGPDGEVKA
                     HFATRWRGRK"
BASE COUNT          380 a          502 c          462 g          376 t
ORIGIN      
        1 gcgacgggcg tcttttttgc gactgcatct ccgacatccg ctacaaacga ggaaggcaga
       61 atggatccca ttcctccgag cattatgaag tctgggttaa ggatactgac gaggttggcg
      121 atccccgtcg cgagaaactc ccccatctcc tcgagaacac ggcgcgcagc gtcgttccct
      181 tcctggaaca acctgagaag cgctctgaat ttttcgcgga aatcgcttcc cggcaaggag
      241 gctcccaggt tctcgaagtt cctcacaaca gtgtctatgg aaacgaacgt ctcgaaacac
      301 cctcggttgc cgcagtggca gagagggcct gaaggatcga ccgtcatatg tccgatttca
      361 cccgccgtat tactgagtcc atggatgatc ttatccccca tgaggatggc gcctcccacc
      421 ccctgcgaaa gatagacata gacgccgctg tgcagcccgg agactccgcc gttccggaac
      481 atctcggcga tcagggagag tttggcctcg tttgcggtga acacagtgag ctccatttcg
      541 ggaagcaact tcccgagacg cgcttcaaaa tcgacgtttt tccaccccat atgcggaaga
      601 gagcgcagca cgttgctccc ccactccacc attcccggaa cggcgacgct cagcgcacaa
      661 tacgccgtat gagggaagga gaacgctttc tttcgcaggc tcccggcgaa aacagagaaa
      721 aattcgtcct ccttcggcgg cgtgtcaaat tcgtcaagca cttcaaaaga agcgtcgaga
      781 aaccctttcg cgatccgggt tttctcgacg ccgatctctg caatcagcgc aacggcagca
      841 tcacggtata cgcggagcat gatggacttt ctccccggat ggtcggcgtc gcgcactcca
      901 tgttcaatga cgagcccttt ttcaaggagc gaggtgataa tcttggtcac tgtgcttgga
      961 tcaagcccgg cgaggcgggc aagttcccgc ctgctcacac cgggagtggt tcggataagg
     1021 ttgagcaagg tcagtctgtt cgaaagaccc attttttcag cgttgatgct ccggattctc
     1081 atcccgtccc tcctctgcgt cgaatcaata cggcggctcg cggtacggag attagtttat
     1141 tgatgaaaca aaccaattct agagagaaag atgagagttg tcaatggcga attttctttc
     1201 agaagtttat ttccggcctc tccaccgtgt ggcgaaatgc gccttcactt ccccgtccgg
     1261 cccttcgatt aagtgaaaga aagccttttc gccttgatcg gccggaagca acgcggtcct
     1321 gacgcgcacg tcgccgtttt tctccacggg tttcaggtat tcggcctcca cttttaccag
     1381 ctcgtactcc gcggccactg catccggcgc agattcgaca gcccaagaga agtagacggc
     1441 gttgttcaca tgggcgtttc ggtcaagatc ccatctgcgg acggagaaaa atgtatcgta
     1501 ggcaaagtcc tcaactacag gaagagaagg agcgtctccg tccatcgggt aggcctctct
     1561 cgtgtagacc tccgtggtgt gccggtcaag cctgagggga cgtccgcgca gagtatcgat
     1621 aaggatccag ctcgtccatg cggacgcaac aggtccagct gcgtcgcgaa cctcgaaagc
     1681 gcggatcgaa tgcagatttc gccgcggtcc gtgccacgtc
//
LOCUS       DAOPKU010000635         1707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_83537_length_1707_cov_2.743341, whole genome shotgun sequence.
ACCESSION   DAOPKU010000635 DAOPKU010000000
VERSION     DAOPKU010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83537_length_1707_cov_2.743341"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PK849_14440"
     CDS             complement(<1..579)
                     /locus_tag="PK849_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPE67378.1"
                     /translation="MLKHTRMSDPELFDIVVEELDRQEHNIEMIASESTESEELLELS
                     GCVFANKTTEGYPGARFQAGSQIADKMERLGIERAKALYGADHANLQPYSGSTANYSV
                     YAAALKPGDCVLGMRLDHGGHLTHGSPANFMSKFYHFVSYGLNPQTEQIDYDELEALA
                     KEHKPRLIIAGGSAYPRLIDYERIANIARSAGG"
     gene            complement(841..>1707)
                     /locus_tag="PK849_14445"
     CDS             complement(841..>1707)
                     /locus_tag="PK849_14445"
                     /inference="COORDINATES: protein motif:HMM:NF027879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MIT C-terminal domain-containing protein"
                     /protein_id="HPE67379.1"
                     /translation="DRYRERFTLSSDISTRDRDGVNKTFSGLMKILFPEGNASAEEME
                     MLLRFAVEGRKRVKDQLLRIDSTYGSVRFCYTDTTGKTKTVTTLEEEEYPHYYHKTVA
                     EGDDEVGRGAVQEQVKVPASPEAPLQEKHLTFQENQKGVSYDALLGPYLEGATKITVT
                     DPYIRLFYQVRNFMEFLETIVRRKAREDEIEVHLITVEDEFKADSQHESFEKIQEASV
                     ALGVAFTWEFDGSGTLHARHITTDHGWKISLDRGLDIFQHYEMNNAFSFANRLQAFRS
                     CKAFEATFIRVD"
BASE COUNT          318 a          530 c          492 g          367 t
ORIGIN      
        1 gccgccggcg gaacgcgcta tattcgcgat acgctcgtag tcgatcaggc gcgggtaggc
       61 gctgccgccc gcgatgatca gcctgggctt gtgctccttc gccagcgcct ccagttcgtc
      121 gtagtcgatc tgctcggtct gcgggttcag cccgtacgaa acgaagtgat agaatttgct
      181 catgaagttc gccggagagc cgtgcgtcag gtgcccgccg tggtccagcc gcatcccgag
      241 cacgcagtcg cccggcttga gcgccgccgc gtacacgctg tagttggcgg tggagccgga
      301 gtaaggctgc aagttcgcgt ggtccgcccc gtagagcgcc ttcgcgcgct cgatccccag
      361 ccgctccatc ttgtcggcga tctgcgaccc ggcctggaag cgcgcccccg gatacccctc
      421 ggtcgtcttg ttcgcgaaga cgcaccccga aagctccagc agctcctcgc tctcggtgct
      481 ctccgaggcg atcatctcga tgttgtgctc ctgccggtcc agctcctcga cgacgatgtc
      541 gaacagctcc ggatcggaca tccgcgtatg tttcagcatc cctcaacgac ctcctgaaat
      601 atcgggtccg gaaacgaaaa aagtgaacgc ggacccgcag tcttaaaacg aggatactcc
      661 aagaaggcgg aaggggcaag ggggcggagt ggccgtggat cttgaagaag cgcttccctg
      721 acacgcggaa gcggatactc tcgatttgaa aaggacagtc ggcgattgcc ctttttgaga
      781 ttcgccaatc attgattagc gaatcgatgg cgaagacacc tggaaaaacc cctcgcttta
      841 tcaatccacc ctgatgaacg ttgcctcgaa cgccttgcag gagcggaatg cttgcaggcg
      901 gttggcgaag gagaaggcgt tgttcatctc gtaatgctgg aatatatcca gtccccgatc
      961 gagggagatc ttccagccgt ggtcggtcgt tatatggcgg gcatgaagcg tgccgctgcc
     1021 gtcgaactcc caggtgaacg ctacgcccag agcgaccgag gcttcctgta tcttctcgaa
     1081 gctctcgtgc tgggaatcgg ccttgaattc gtcctcgacc gttatcagat gcacctcgat
     1141 ctcatcttcc cgggcctttc gcctgacgat cgtctccagg aattccatga agttgcgcac
     1201 ctgatagaag agccgtatgt acgggtccgt caccgtgatc ttcgtcgcgc cctcgaggta
     1261 cggccccagc agcgcgtcgt acgatacccc tttctggttc tcctgaaagg tgagatgctt
     1321 ctcctgaaga ggcgcttccg gggacgcagg aaccttcacc tgctcctgga cggctccacg
     1381 cccgacctca tcatcgccct ccgcgaccgt cttgtggtag tagtgcggat actcctcctc
     1441 ttccagcgtc gtgaccgttt tcgtcttccc ggtcgtatcg gtataacaaa aacgcacgct
     1501 cccgtaggtg gagtcgatcc tcaggagctg atccttcacc ctctttcgcc cttcaacggc
     1561 gaatcgcagc aacatttcca tctcctcggc ggaggcgttt ccctccggaa agaggatttt
     1621 catcaggccg gaaaaggtct tgttgacgcc gtcgcggtcg cgggtcgaga tgtcggagga
     1681 gagcgtgaag cgctccctgt accggtc
//
LOCUS       DAOPKU010000636         1705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_83664_length_1705_cov_4.000606, whole genome shotgun sequence.
ACCESSION   DAOPKU010000636 DAOPKU010000000
VERSION     DAOPKU010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83664_length_1705_cov_4.000606"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK849_14450"
     CDS             <1..399
                     /locus_tag="PK849_14450"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPE67380.1"
                     /translation="EKGDVLICEGGYPGRAAIWENEYPIYFQKALHRVRFHKPEHNKW
                     FVYFLFACFQNGTLREYFSGTGIQHFTGEALSRFVVPVPPTQQIQAEVAKFDALVVET
                     QRLASIYHRKLAALDELKKSLLHQAFTGQL"
     gene            438..>1705
                     /locus_tag="PK849_14455"
     CDS             438..>1705
                     /locus_tag="PK849_14455"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPE67381.1"
                     /translation="MNEAETRAEYIDPALKTAGWGEAGGSRVRREYVISPGRIEGRGR
                     RGKSLTADYVLEFRNAKLAVVEAKSRDEPLTAGAAQAKNYAEKLQVRFAYATNGQGIY
                     EIDMMTGREREIPFYPSPEDLWRRIHAVQNEWRDRFFEVPFEDKSGSHPGRYYQDIAV
                     ERVLHSVAEGKKRILLTLATGTGKTFIAFQVAWKLFHSRWNLSREPARRPRILFLADR
                     NILADQAYNAFSSFPEDAMVRISPADISKKGRVPKNGSLFFTIFQTFMTSPRRDEESS
                     FCFGEYAPDFFDFIIVDECHRGGANDESAWRGILEYFAPAVQLGLTATPKRAENIDTY
                     AYFGEPVFVYSLKEGINDGFLTPFRVKQIATTLDEYFYTPDDTLVEGEVEQGKIYTES
                     DFNRIIEIKERERKRVRIFMEQIDQREKTLV"
BASE COUNT          407 a          472 c          489 g          337 t
ORIGIN      
        1 gaaaaaggag acgttctcat ctgtgaaggc ggttatcctg ggcgtgctgc catttgggag
       61 aatgagtatc ccatatactt tcagaaagcg ctgcatagag tacgttttca caaaccagag
      121 cacaataaat ggtttgtgta ttttcttttt gcatgttttc aaaacggaac cctgcgagag
      181 tatttcagcg gtactggcat acaacatttt accggagaag cactctccag atttgttgtt
      241 cccgtgcctc ctacgcaaca aatccaagct gaagtcgcaa aattcgacgc gctcgtcgta
      301 gaaacccagc gccttgcctc catttaccat cgcaagctcg ccgcgctgga cgaactgaaa
      361 aaatcgctgt tgcaccaggc gttcaccggg caactctgaa gacacaagcc gtttgaaccg
      421 cggagaggtg atcctctatg aacgaagccg aaaccagagc cgaatacatc gacccagcgc
      481 tgaaaacggc gggatggggt gaagccggag gcagccgcgt cagacgggag tatgtcatct
      541 cgccggggcg aatcgaaggg cgcggaaggc ggggcaagtc gctgaccgcc gactacgtgc
      601 tggagttccg gaacgcgaag ctggccgtgg tcgaggcgaa gtcgcgggac gagcccctca
      661 ccgcgggcgc cgcccaagcg aagaactacg ccgaaaaact gcaagtgcgt tttgcctatg
      721 ctacgaacgg gcaggggatt tacgagatcg acatgatgac gggcagggaa cgggagattc
      781 cgttctaccc ctccccggaa gatctatggc ggcgaatcca cgccgtgcag aacgagtggc
      841 gcgaccgttt tttcgaggtc cctttcgagg ataagagcgg ttcccacccc ggacggtact
      901 accaggacat cgcggtcgag cgggtgctgc actcggtagc ggaggggaaa aagcgcattc
      961 tgctgacgct cgccaccggt acgggaaaga ccttcatcgc ctttcaggtt gcatggaagc
     1021 tctttcacag tcgatggaat ctttcccgcg agcctgcgag acgcccccgc atcctcttcc
     1081 tcgcggacag aaacatcctc gccgaccagg cgtacaacgc attctcctcg ttcccggagg
     1141 atgcgatggt tcggatctcc cctgcggaca tttcaaaaaa aggacgcgtg ccgaagaacg
     1201 gcagtctttt cttcaccatc ttccagactt tcatgacaag ccctcgaagg gatgaagagt
     1261 cctctttctg tttcggcgag tacgctcccg atttcttcga tttcatcatc gtcgacgagt
     1321 gccacagagg cggggccaac gacgagagcg cgtggcgcgg cattctggaa tactttgcac
     1381 cggcggtgca gctcggactc accgcgacgc cgaagcgggc cgagaacatc gacacctacg
     1441 cctacttcgg agagcccgtc tttgtgtact ctctgaagga gggaatcaac gacggttttt
     1501 tgacgccttt cagagtgaag cagattgcga ccacgctcga cgagtacttc tacacccccg
     1561 acgatacgct cgtcgaaggg gaggtggagc aggggaaaat ctacaccgag agcgacttca
     1621 acaggattat cgagatcaag gagcgcgaaa gaaagcgcgt gcgcattttc atggagcaga
     1681 tcgaccagag agagaagacg cttgt
//
LOCUS       DAOPKU010000637         1696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_84301_length_1696_cov_3.367459, whole genome shotgun sequence.
ACCESSION   DAOPKU010000637 DAOPKU010000000
VERSION     DAOPKU010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84301_length_1696_cov_3.367459"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PK849_14460"
     CDS             <1..478
                     /locus_tag="PK849_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE67382.1"
                     /translation="LDAKLRVRMRGEIRRLQREFGITALYVTHDQEEALALSDRVAVM
                     EGGRIVQTGTPQEVYGAAANSFVADFIGRSNILVLEDGSKVAVHPEDIRLSSGGEGVE
                     GVVFDRQFKGAVVTWFVDAGGRTVEADLPGRSGDAWRTGERVRISFDRGSATVLRE"
     gene            complement(518..1294)
                     /locus_tag="PK849_14465"
     CDS             complement(518..1294)
                     /locus_tag="PK849_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPE67383.1"
                     /translation="MKTITAGVDIGSTGVKAVLFDGSILARSILPTGWSPAETGERAL
                     ADLLLERGLLRTDLVRVTATGCGRKIFREADDIITEITCHAAGAFFLEPEVRSVLDLG
                     GQDSKAIALADDGSVADFLMNDKCAAGTGRFLSMTANLLEVDLRWFDALGEGETHPIS
                     SMCAVFAESEMVGLLASGVEKEALARGVLDSIASRAAGMLQRVGARGPVLFTGGLARC
                     APLLRMLERKTGLEFRSSEHSQTAGALGAAVLGAMKARVG"
     gene            complement(1296..>1696)
                     /locus_tag="PK849_14470"
     CDS             complement(1296..>1696)
                     /locus_tag="PK849_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPE67384.1"
                     /translation="SLHKVIRAVEEQGGGVVVAFENCGNLKSSTDLADESRPPMEAIA
                     DRYLHIPCSCMSPNPRRLEFLSRFREEYAVDGVVDVILQACHTYAVESFSVREHLKGL
                     GIPYIAVETDYSQGDEGQLSTRLNAFLEML"
BASE COUNT          287 a          583 c          567 g          259 t
ORIGIN      
        1 cctggacgcg aagctccgcg tgcggatgcg gggcgagatc cggcgtttgc agcgggagtt
       61 cggcatcacc gcgctctacg tcacgcacga tcaggaggag gcgctcgcgc tctcggacag
      121 ggtggccgtg atggaggggg ggcgcatcgt ccagaccggc acgccgcagg aagtttacgg
      181 cgcggcggcg aacagcttcg tcgcggattt catcggcagg agcaatatcc tggtcctgga
      241 ggacggaagc aaggtcgccg tccacccgga ggacatccgc ctttcgagcg gcggcgaggg
      301 tgtggaaggc gtcgtcttcg accgtcagtt caagggcgcg gtcgtcacct ggttcgtcga
      361 cgccgggggc cgcaccgtcg aggcggacct tcccggcagg tccggcgacg catggaggac
      421 gggcgagcgc gtccggatat ccttcgacag ggggagcgcg acggtactga gggagtaagg
      481 agtttgtccc tgacaaaacg cgtccggcgg cgaccgctta cccgacgcgc gccttcatcg
      541 cgcccagtac cgccgcgccg agcgctccgg ccgtctggga gtgttcggaa gaacggaact
      601 cgagccctgt cttccgttcg agcatccgca gcagcggcgc gcaccgggcg agcccgccgg
      661 tgaacagcac cggaccgcgc gcgccgacgc gctgcagcat ccccgccgcc cgcgacgcga
      721 tggagtccag cacgccccgc gccagcgcct ccttctccac gccggaggcc agtagcccga
      781 ccatctccga ctccgcgaag acggcgcaca tgctcgaaat agggtgcgtc tcgccctcgc
      841 cgagggcgtc gaaccagcgg aggtcgacct ccagcagatt cgcggtcatc gagaggaaac
      901 gccccgtccc ggcggcgcac ttgtcgttca tcagaaagtc ggcgacgctt ccgtcgtccg
      961 ccagcgcgat cgccttgctg tcctgcccgc cgaggtcgag caccgaacgg acctccggct
     1021 ccagaaagaa ggcgcccgcc gcgtggcacg tgatctcggt gataatgtcg tcggcctcgc
     1081 ggaagatctt tctgccgcac cccgtcgcgg tgacgcgcac gaggtccgtc cgcagcagcc
     1141 cccgctccaa gagaaggtcg gcgagcgccc gctctcccgt ctcggcgggg ctccaccccg
     1201 tgggaagaat ggagcgggca aggatggagc cgtcgaagag gacggccttc acgcccgtcg
     1261 aaccgatgtc tacacccgcc gtgatcgtct tcatgtcaca gcatctccag aaaggcgttc
     1321 agccgggtcg agagttgccc ctcgtccccc tgcgaatagt cggtctccac cgcgatataa
     1381 ggaattccga gccccttcag atgctcgcgc acggagaagc tctccaccgc gtaggtgtgg
     1441 cacgcctgca gaatcacgtc gaccacgccg tcgacggcgt actcttcgcg gaagcgggag
     1501 aggaactcca gccgccgggg gttcgggctc atgcacgaac aggggatgtg cagatagcgg
     1561 tcggcgatcg cctccatcgg cggacgggac tcgtccgcaa gatcggtgct gctcttcaga
     1621 ttgccgcagt tttcgaaggc gacgaccacg ccgcccccct gctcctccac cgcgcggatc
     1681 accttgtgca ggctct
//
LOCUS       DAOPKU010000638         1694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_84465_length_1694_cov_2.089689, whole genome shotgun sequence.
ACCESSION   DAOPKU010000638 DAOPKU010000000
VERSION     DAOPKU010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84465_length_1694_cov_2.089689"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PK849_14475"
     CDS             complement(<1..1382)
                     /locus_tag="PK849_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPE67385.1"
                     /translation="MGRRSFSSADATFEVRFSREAWETMLAEASRDRDDSAPMRETGG
                     ILIGSWKEKNGRTVVTVRRSSGPGPKSIRETHVFSPDLEYFRDRSGYYATRKNWRYLG
                     EWHRHPGSFHALSGTDLRMASELLRDEKWPFLLLPLVNIEDGKLVVTMAIAVSSESGR
                     AEIVSLGRITAGTPYSHKGGRDRMKVFVDRDWIERFAQGQENVCEYKGFRNPGESWVF
                     LPLPGTGNATLRLIREGTETSLPDTENIVTAVLGTDGDAICYTVREGQILEIPLVAVA
                     PQEDVYSRNSGILETRELAGKKALLVGCGSVGSTMAMELVRAGIGSIVLVDPDVLEAA
                     NICRHQAGLQHLGRRKADVVRDLLLSINPSANVEVHAVDVCGDMETMELMSDIAAGCD
                     VLLCTTDTDESRIFVNDLSISCGVPSIQAGLHERAASGIVQTVRPGGSACFMCHRERV
                     LRGTRPEGGA"
     gene            complement(1382..>1694)
                     /locus_tag="PK849_14480"
     CDS             complement(1382..>1694)
                     /locus_tag="PK849_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67386.1"
                     /translation="SAFSLSYLESGRSALMRTPFGKRNGNGNQWSNENGSSARAFDDD
                     LFSFDGGRDEDIRDFTRQMLDGDNSAAVITRDGRILPKNHALKSGDKDFTELYKERVW
                     G"
BASE COUNT          334 a          570 c          424 g          366 t
ORIGIN      
        1 taggctcctc cttcgggacg ggttccgcgc agcacccgct ctctatggca catgaaacag
       61 gcgctcccgc ccggccgcac tgtctgaaca ataccgctcg ctgcccgctc gtgcagtccc
      121 gcctgtatgg agggtacccc gcaggatatc gaaagatcgt tcacaaaaat acgcgattcg
      181 tcggtgtccg tggtgcacag cagcacgtcg caaccggcgg cgatgtcgct catcagctcc
      241 atcgtctcca tgtcgccgca tacgtccacc gcatgaacct ctacatttgc cgatggattg
      301 atggagagca gcaaatccct gaccacgtcg gccttcctcc gccccagatg ctgcaatccc
      361 gcctggtgac ggcagatgtt cgccgcctcc agaacatcgg ggtccaccag cacgatgctc
      421 ccgatgccgg ccctgacaag ttccattgcc attgtcgatc ccacgctgcc gcatccgacg
      481 agaagcgctt tcttcccggc cagttcgcgg gtctccagaa tgccgctgtt ccggctgtac
      541 acatcctcct gcggggcaac tgcgacaaga ggaatctcca gaatctggcc ctcccgcacg
      601 gtgtagcaaa ttgcgtcgcc gtccgtaccc aaaacggctg ttactatgtt ctcagtatcc
      661 ggcaggctcg tttcggttcc ctcgcggatc aagcgcaggg ttgcgttccc cgtcccggga
      721 agcggaagaa aaacccagct ttcgccagga ttccggaacc ctttgtattc gcacacattc
      781 tcctgtccct gtgcaaaacg ctctatccag tctctgtcga cgaatacctt catcctgtcg
      841 cgtcctcctt tgtgcgaata cggcgttccg gccgtaattc tgccgagaga gacgatctcc
      901 gccctcccgg actcgcttga aaccgctatg gccattgtga ctacaagttt gccatcttca
      961 atattcacga gcgggagaag caggaacggc cacttctcgt cccgcagcaa ctccgaagcc
     1021 atccttaaat cggttccgct gagcgcatga aaagagcccg ggtgcctgtg ccactccccc
     1081 agatagcgcc agtttttccg cgtcgcatag tagcctgaac gatcccgaaa atactccaga
     1141 tcgggtgaga acacgtgtgt ctcccggata ctcttcggtc ccggtccgct gcttctgcgc
     1201 accgtaacca cggtacggcc gttcttctct ttccacgaac caatgagaat acccccggtt
     1261 tccctcatgg gagcagaatc gtctctgtct ctcgaggcct ccgcaagcat cgtctcccac
     1321 gcctcgcggg aaaaccgcac ctcgaacgtc gcgtcggcgg aactgaacga tcgcctgccc
     1381 atcagcccca caccctctcc ttatacagct ccgtgaaatc cttgtcgccg gacttcagcg
     1441 cgtggttctt cggaaggatg cgaccgtcgc gtgtgattac ggccgccgaa ttgtcgccgt
     1501 caagcatctg gcgggtgaaa tcgcggatat cttcgtcgcg tcctccgtca aaggagaaca
     1561 gatcgtcgtc gaacgccctt gcggaagaac cgttctcgtt gctccactga ttcccgtttc
     1621 cgttccgctt gccgaaaggt gttctcatca aagcagacct ccctgattcg agataggaga
     1681 gcgaaaatgc cgaa
//
LOCUS       DAOPKU010000639         1692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_84563_length_1692_cov_5.525351, whole genome shotgun sequence.
ACCESSION   DAOPKU010000639 DAOPKU010000000
VERSION     DAOPKU010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84563_length_1692_cov_5.525351"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PK849_14485"
     CDS             complement(<1..473)
                     /locus_tag="PK849_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67387.1"
                     /translation="MKARNGATKTLQRRGGIRRYGALLSTVVFLWAAWAGCAHAENEA
                     KAYPPVTWTVTEASDNPSSPGENTLRGIIGKATDLDIVVFGPGIAEISLQGELVIDKV
                     LTIAGSSTQQVTIRQTAAGARVLSVSSGKSAGLQYLTITGGRRAGYGDGGGILNNG"
     gene            complement(656..1549)
                     /locus_tag="PK849_14490"
     CDS             complement(656..1549)
                     /locus_tag="PK849_14490"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPE67388.1"
                     /translation="MDTRILTTFRRIVTSGGFSSAASELGYAQSTVSVHIQTLEETLG
                     GPVFDRAGRKPVLTHLGKELLGLSEEMLRLEERVRNLASGSSEPSGELNVATGESVMV
                     SRLGESLARFKQRYPHVVLRMRNSYCPAMIDWVLRGESDLAVLIMPPLNAPDLETRPL
                     RREEMVFVAAPGKDRNLLLDALDRGKLDECFIHTEQGCSYRVFIENFFRSKGLVLEKT
                     MELWSMEGIKKCVQSGLGISFVPLMYVRGALADGSLEQLAAPPLEESFVIQAAWRKTG
                     ALSPAAREFLAILEEDALSWR"
BASE COUNT          312 a          545 c          476 g          359 t
ORIGIN      
        1 ccgttgttga gaatgccgcc gccgtcgccg tatccggccc gtctgcctcc cgtgatcgtc
       61 aggtactgca ggcccgcgct ctttcccgag gaaacggaaa gcaccctggc gcccgccgcc
      121 gtctgcctga tcgtgacctg ttgcgtggag gagcccgcta tcgtcagtac cttgtcgatg
      181 accaactctc cctggagcga aatctccgcg atgcccggac cgaagacgac gatatcgaga
      241 tcggtcgcct tcccgatgat tccgcgcagc gtgttctcgc cgggagagga ggggttgtcg
      301 gaagcctccg tcaccgtcca cgtcaccgga ggataggctt ttgcttcgtt ctccgcgtgc
      361 gcgcaccccg cccatgccgc ccaaaggaac acgacggtcg aaagcaacgc cccgtaccgt
      421 cgtattcctc ctcttctttg cagtgtcttc gttgctccgt tgcgcgcctt cattcctcct
      481 accacccttc ttgttgcaaa atctcccctg gtcccgggag ttgaaagccc gtatccatag
      541 cgtcgaaaaa ttcgccctta tgccaaaagc aataataaac ctttgcaccg tgtgagagtc
      601 aaggggaagt tttcatgttc gagcgaaagt acacggcgtc ccggagatca gttcttcatc
      661 tccaggaaag ggcgtcctct tcgaggatgg cgaggaattc cctcgcggcc ggggagagcg
      721 cccccgtttt tctccatgcg gcctggatga cgaagctctc ctcaagcggc ggcgcggcga
      781 gctgttcgag ggacccatcg gcgagggcgc cgcggacgta catgaggggg acgaaggaga
      841 tgccgaggcc gctctggacg cactttttaa ttccctccat gctccacagc tccatcgtct
      901 tttcgagtac cagacctttg ctccggaaga agttctcgat gaagactctg tagctgcacc
      961 cctgctccgt atggatgaag cactcgtcga gcttcccccg gtcgagcgcg tcgagcagca
     1021 ggttccggtc tttccccgga gcggcgacga agaccatctc ctcccgccgc agcgggcgcg
     1081 tctccaggtc cggcgcgttg agcggcggca tgatgagcac tgccagatcg ctctcgccgc
     1141 gcagaaccca gtcgatcatc gccggacagt acgagttgcg catccgcagg acgacatgag
     1201 gatatcgctg tttgaagcgg gcgagggatt cccccaacct agacaccatg acgctctccc
     1261 ccgtcgccac gttcagttcg cccgagggtt cggatgagcc agacgccagg ttccggacgc
     1321 gctcctccag gcggagcatc tcttcggaga gcccgagcag ctccttcccc agatgcgtca
     1381 ggaccggctt tctgcccgct ctgtcgaaga cggggccgcc gagcgtctcc tccagcgtct
     1441 gaatatggac ggagaccgtc gactgcgcat accccagctc gctcgccgcg ctggagaagc
     1501 cgccgctcgt cactatcctg cggaacgtgg tcagaattct ggtatccatg ccatgcctcc
     1561 cgaaggcctc tttcgaggcg tgatatcgtt tttatcgatg agaaccatct tttatttcca
     1621 ttttaccgat tctacttcct tctgtatagt gggctcaaaa ggagggggta ttcgtgaaga
     1681 aaaaattgaa cg
//
LOCUS       DAOPKU010000640         1692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_84567_length_1692_cov_4.639585, whole genome shotgun sequence.
ACCESSION   DAOPKU010000640 DAOPKU010000000
VERSION     DAOPKU010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84567_length_1692_cov_4.639585"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PK849_14495"
     CDS             complement(<1..938)
                     /locus_tag="PK849_14495"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPE67389.1"
                     /translation="MSFFQGVPVAIRGSGMYAPRKVVTNNDLSQIVETSDEWIRERTG
                     IQRRHIAEPGELTSTIAEKAGRAALENAGILPSELDMVIVATNSPDTLFPGVAPKVQG
                     LLKAENAGAFDVQSGCTSGVYSIVLAVNGIASGLWKNVLVIGAEVLSPLIDWTDRNTC
                     VLFGDGAGAMVLAPSNGNGRFISAQLKSDGTKHDQITFLGGLIENPASHETIDQKKHY
                     VAMKGNDVFKFVNRVIPPFLKTLCEDAGMLPEGIDWWFFHQANQRIIERTAERLGINP
                     ERVFINLDEYGNTSAASVFIALSEFMKQGKLLRGQKL"
     gene            complement(952..>1692)
                     /gene="plsX"
                     /locus_tag="PK849_14500"
     CDS             complement(952..>1692)
                     /gene="plsX"
                     /locus_tag="PK849_14500"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPE67390.1"
                     /translation="MRLAMEMVRSGEASGCVSSGNTGAIVAGGVLVVGRISGIDRPAM
                     GVALPTLNKYTFLLDVGANVRNKPYHLFQFAHMGNIYSKRILGVTTPQIALLSNGSED
                     IKGDEVISGAKTLIKESALNFSGYVEGDDIPYSRADVVVCDGFSGNIVLKFAEGLMTA
                     VASMLKEEIGHRILPKVGMVFMLPMMKDLIARFHYEKHGGTPLLGVNGAVIKAHGRSK
                     APAIVSALRVARNFVKQNGVELIREELG"
BASE COUNT          397 a          468 c          408 g          419 t
ORIGIN      
        1 agtttctgtc cccgaaggag tttcccttgt ttcataaact cggacagagc gataaagacg
       61 gatgccgcgg atgtgttgcc gtattcgtca agattgatga atactctctc gggattgatg
      121 ccgagacgct ctgctgtgcg ttcaatgatt cgctggtttg cctgatgaaa aaaccaccaa
      181 tcaattccct caggcaacat gcctgcgtct tcgcacagtg ttttcaaaaa cgggggaata
      241 actcgattga caaatttaaa gacgtcattc cctttcattg cgacatagtg ttttttctga
      301 tcgatggttt cgtgcgaggc agggttttcg ataagccctc cgaggaacgt tatctggtcg
      361 tgtttcgtgc cgtccgactt cagttgagcc gagataaagc gtccgttgcc gttggaagga
      421 gcgagaacca tcgcgcccgc gccgtcgccg aaaagaacgc acgtattccg gtcagtccag
      481 tcaataagcg gcgaaagcac ctcggcgcca atgacgagaa catttttcca aagacctgaa
      541 gctataccgt taacggcgag gactatagag tacactccgc tggtgcatcc cgactgaacg
      601 tcgaacgccc cagcattctc tgctttaagc aacccctgga ctttcggggc tactcccggg
      661 aatagagtat ccgggctgtt cgtggcaaca atgaccatat cgagttcaga aggcaagata
      721 ccagcatttt caagtgctgc acgccctgcc ttttcggcga ttgtgctcgt gagctctccc
      781 ggttcagcaa tgtgtcttct ctgaatgcca gtcctttcac gaatccattc atcgcttgtt
      841 tcgactattt gtgataagtc gttattcgtc actacttttc tcggggcata cataccgcta
      901 ccgcgaatgg cgacgggcac tccttgaaaa aaagacatga gaagacctcc tttaccctaa
      961 ctcttccctt atcagctcaa cgccgttttg cttaacgaag ttcctggcaa ctctcagcgc
     1021 actcacgata gccggcgcct tcgaccgtcc atgagccttt attacagctc cgttgacgcc
     1081 cagaagcggc gtgccgccgt gcttctcata gtgaaagcgt gcaatgagat ctttcatcat
     1141 agggagcata aagaccattc ccaccttggg aagaatccga tggccgatct cttctttgag
     1201 catagaagcg actgcagtca tcaggccctc tgcgaatttg agaacgatgt ttcccgagaa
     1261 gccgtcgcag acaaccacgt ccgccctgct gtacggaatg tcgtctccct caacgtatcc
     1321 cgaaaaattg agcgcgctct ctttaataag cgtttttgcg ccggagatca cctcgtcgcc
     1381 ctttatatcc tccgagccgt ttgagagcag cgctatctgc ggcgtcgtaa cgcccaggat
     1441 gcgctttgaa tagatattac ccatgtgagc gaactgaaaa agatgatacg gtttatttcg
     1501 tacgtttgcg cccacgtcga gaagaaaggt gtacttgttc agcgtgggga gcgcaacacc
     1561 cattgcagga cggtcgatcc cggaaatcct gcctacgaca agcactcctc cggcaacaat
     1621 cgcgcctgtg ttaccggagg atacacatcc ggatgcttcg ccggaacgaa ccatctccat
     1681 cgcaagacgc at
//
LOCUS       DAOPKU010000641         1685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_85081_length_1685_cov_4.951534, whole genome shotgun sequence.
ACCESSION   DAOPKU010000641 DAOPKU010000000
VERSION     DAOPKU010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85081_length_1685_cov_4.951534"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..>1685)
                     /locus_tag="PK849_14505"
     CDS             complement(29..>1685)
                     /locus_tag="PK849_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPE67391.1"
                     /translation="IDTPGHADFSGEVERILSTVDSVLLLVDAGEGPMPQTRYVLSHA
                     LRMGIKPIVYINKVDRKEADPVLALNATFDLFFELGATDEQADFPVLYGSGLAGWAVK
                     ELSEIEGGESKGMDALFETVIEYVEPPLADEESPFLLQVSTLAWNEYTGRMGCGKILQ
                     GKIRKGEEFLRTTTAWEDRADKENENFVIAGKDRTKAVQIFVTRGLERLEVEEAGAGD
                     IVWISGPKEIGIGDTFCSPDLENFPLPPLDIEEPTVSMFFLVNNGPFSGQEGQAITLR
                     QLKARLEREMHVNVALRMEDLGRPDGVKVSGRGELQLAILIEEMRREGMEFCVSKPEV
                     ITEERDGKTFEPMEDLVVDIPEEYQGVVFEKLSRRKARVQEIQNLRTGLMRIFFAVPT
                     RGLIGYRGEFLTDTRGLGIMSSRFSGYAPWVGEITPRNRGALVSLDTGEATSYQLENL
                     QERGVLFISPMDKIYSGMIVGENARPGDMPCNPTKRKQATNHRSSTKDFTIKLDVPRK
                     MTLEKALEWISEDELVEVTPKSIRLRKSILDDNDRRKARRISA"
BASE COUNT          278 a          525 c          515 g          367 t
ORIGIN      
        1 ccccgttgga ggagcggcgc ttgtcttttt atgcgctgat gcgtcgagcc tttcttcgat
       61 cgttgtcgtc caggatggat ttgcgaagac ggatcgactt gggcgtcacc tcgacgagtt
      121 cgtcctcgga gatccattca agggcttttt cgagggtcat tttcctcggg acgtcgagct
      181 ttatagtaaa gtccttggtg gaggacctgt ggttggtcgc ctgcttgcgc ttcgtcgggt
      241 tgcagggcat gtcgcccggg cgcgcgtttt cccccacgat cattcccgag tagattttgt
      301 ccatcggcga gatgaagagg acgccccgct cctggagatt ttcaagctgg tagcttgtgg
      361 cctcgccggt gtccaggctg accagcgcgc ccctgttgcg cggcgtgatc tcgcctaccc
      421 acggggcgta cccggagaac cgggacgaca tgatcccgag ccctctcgtg tcggtcagga
      481 attccccccg gtagccgatg aggccgcggg tggggacggc gaagaagatg cgcatcagtc
      541 ccgtacggag attctggatc tcctgcacgc gcgccttgcg tcgcgacagc ttctcgaaga
      601 cgacgccctg gtactcctcg gggatgtcca cgacaagatc ctccatcggc tcgaaggttt
      661 ttccgtcgcg ctcctcggtg atgacctccg gtttggagac gcagaactcc atcccctcgc
      721 ggcgcatctc ctcgatgagg atcgcgagct gcagttcccc gcgtccggag accttgactc
      781 cgtcgggacg tcccaggtct tccatgcgga gggcgacgtt gacgtgcatt tcgcgctcca
      841 gccgcgcctt gagctgacgg agcgtgatgg cctgtccttc ctgccccgag aaggggccgt
      901 tattcacaag gaagaacatc gagaccgtcg gctcctctat atcaagcggg ggcagcggaa
      961 agttctcaag atccggcgag cagaaggtat cgccgatgcc gatctccttg gggccggaga
     1021 tccagacgat atcgcccgct cccgcctctt ccacttcaag acgctcaaga ccgcgcgtga
     1081 cgaagatctg gacggctttt gtccggtctt ttccggcgat gacgaagttc tcgttctcct
     1141 tgtcggctct gtcctcccag gcggtcgttg tgcggaggaa ttcctccccc ttgcgtatct
     1201 tcccctggag gatcttcccg caccccatgc gtccggtgta ttcgttccag gcgagcgtgc
     1261 tcacctgcaa caggaaggga gactcctcgt cggcgagcgg gggctccacg tactcgatga
     1321 cggtctcgaa aagggcgtcc atccccttcg attcgccgcc ctcgatctcg ctcagttcct
     1381 tgacggccca accggcaagt cccgacccgt agaggacggg gaaatccgcc tgctcgtcgg
     1441 ttgcgccgag ttcgaaaaag aggtcgaacg tggcgttgag cgcaagcacc gggtccgcct
     1501 ctttgcggtc caccttgttg atatagacga tcggcttgat ccccatccgg agcgcatgcg
     1561 agaggacgta ccgggtctgt ggcatcggcc cctctcccgc gtccacgagg agcagcacgg
     1621 agtcgaccgt ggagaggatg cgttccactt cgccggaaaa gtcagcgtgt cccggagtat
     1681 cgatg
//
LOCUS       DAOPKU010000642         1684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_85156_length_1684_cov_3.745856, whole genome shotgun sequence.
ACCESSION   DAOPKU010000642 DAOPKU010000000
VERSION     DAOPKU010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85156_length_1684_cov_3.745856"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PK849_14510"
     CDS             <1..515
                     /locus_tag="PK849_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE67392.1"
                     /translation="LTLKDMWPIFMKMARTTGVVFLVIAAASILGWVLTIEQIPEKVA
                     TLMLSVSTNKWVVMGMILLLLLFIGMFMDIAAALIILGPILHPLAVKIGFHPLHFGII
                     MVLALNIALMTPPVGACLFVACGISKLTIEQLSKEIFPFILVVVAVLLLITFVPEIPL
                     FLPRLMGMVH"
     gene            550..>1684
                     /locus_tag="PK849_14515"
     CDS             550..>1684
                     /locus_tag="PK849_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HPE67393.1"
                     /translation="MIPFEKVLSKAEEYKPKMSRFLRDMIALPSESCGEKEVIHRIKA
                     EMEACGYDRVEIDPMGNVLGFIGNGKHLIALDAHIDTVGIGDRTLWTYDPYEGYEDDE
                     IIVGRGASDQEGGMASMVYAGKIIKDLGLPGDFTLMVVGSVQEEDCDGLCWQYIINED
                     KYTPEFVVLTEPTSCNIYRGHRGRMEIKVSTRGLSCHGSAPERGDNAIYKMAPILQEL
                     RALHENLHFDPFLGKGSLTVSEVFFSSPSRCAVADGCTISVDRRLTHGETWEMALQQI
                     RNLPAVKAAGAEVSMYSYDRPSWTGLVYPTECFFPTWVLEENHAACRSMVDGYRDLFK
                     SEPLVDKWTFSTNGVSIMGRYGIPCVGFGPGHEDQAHAPDERTW"
BASE COUNT          328 a          501 c          516 g          339 t
ORIGIN      
        1 acctgacgct gaaggacatg tggccgatct tcatgaagat ggcgcgcacc accggcgtgg
       61 tctttcttgt catcgccgcc gcctcgattc tcggctgggt gctcaccatc gagcagattc
      121 cggagaaggt ggctaccctc atgctcagcg tgagcacgaa caaatgggtg gtcatgggaa
      181 tgatcctgct actcctcctc ttcatcggca tgttcatgga catcgcggcg gcgctcatca
      241 tcctcggccc gatcctgcac cctctcgcgg tgaagatcgg tttccatccg ctgcacttcg
      301 gcatcatcat ggtgctcgcg ctcaacatcg cgctgatgac gcctcccgta ggcgcgtgtc
      361 tcttcgtcgc ctgcggcatt agtaaactga cgatcgaaca gttaagcaag gagatctttc
      421 cctttatcct cgtcgtggtg gcggtgctgc ttctgatcac cttcgtaccg gagatcccgc
      481 tcttcctgcc gaggttgatg ggaatggttc actgagttat taagaatatc gcatgaaaga
      541 aggtattcca tgattccctt cgagaaggtt ttgagcaagg cggaagagta caagccgaag
      601 atgtcccgct tccttcggga catgatcgct cttcccagcg agagctgcgg ggagaaggaa
      661 gtgatccatc gcatcaaggc cgagatggag gcctgcgggt acgacagagt cgagatcgac
      721 cccatgggga acgtgctcgg cttcatcggg aacggcaaac acttgatcgc cctcgacgcg
      781 cacatcgata ccgtcggaat cggcgaccgc accctttgga cctacgaccc ctacgagggg
      841 tacgaggacg acgagatcat cgtcggcaga ggcgccagcg accaggaggg cggcatggcc
      901 tccatggtct acgcggggaa gatcatcaag gatctggggc ttccgggcga cttcacgctg
      961 atggtggtgg gctccgtcca ggaggaggat tgcgacggcc tctgctggca gtacatcatc
     1021 aacgaggaca agtacacgcc ggagtttgtg gtgcttaccg agcccacgtc gtgcaacatc
     1081 taccgcggcc accgcggccg tatggagatc aaggtgagca cgagggggct ctcctgccac
     1141 gggtccgctc cggaacgggg cgacaacgcg atctacaaga tggcgccgat tcttcaggag
     1201 cttcgcgccc ttcacgagaa cctgcacttc gacccgttcc tcggcaaggg aagccttacc
     1261 gtgtctgagg tcttcttcag ctctccgtcg cgctgcgcgg tggcggacgg ctgtaccatc
     1321 tccgtggacc gcagactcac gcacggcgag acgtgggaga tggcgctgca gcagatacgc
     1381 aaccttccgg cggtgaaggc cgccggtgcg gaggtttcca tgtactccta cgaccgtcct
     1441 tcctggacgg gactggtgta ccccacggaa tgcttcttcc cgacgtgggt gctcgaggag
     1501 aatcacgcgg cgtgcaggtc catggtggac gggtacaggg atcttttcaa gagcgaaccg
     1561 ctcgtcgaca agtggacttt ctccacgaac ggcgtctcca tcatgggacg ctacggcatt
     1621 ccctgcgtcg gcttcgggcc gggacacgag gatcaggcgc acgcccccga cgagcggaca
     1681 tgga
//
LOCUS       DAOPKU010000643         1680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_85465_length_1680_cov_5.072615, whole genome shotgun sequence.
ACCESSION   DAOPKU010000643 DAOPKU010000000
VERSION     DAOPKU010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85465_length_1680_cov_5.072615"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..>1680)
                     /locus_tag="PK849_14520"
     CDS             complement(35..>1680)
                     /locus_tag="PK849_14520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HPE67394.1"
                     /translation="IILSVSSSLYIFFTFEAFSMRGTAIPLDITMGAILIVCVLEATR
                     RSVGKELMILALLFLAYGYWGEYIPGVLGHTGFTYKRIVYQLFLSSEGIFGISLGVSA
                     TYIFLFILFGAFLTETGMGRFIKDLAMSLAGRTIGGEAKVSIVASGLMGMINGSAVGN
                     VAATGTFTIPLMTGAGFKPVFSAAVVAVAGTGGMIMPPVMGAASFIMAEFLGLHYGVI
                     MLAAAIPAVLYYLAEYAVIHIEAVKMGMKPIPRDEIIPLTKVMRERGHLLVPIIVIVY
                     LLLIGRTPLYAAFYGIISSIAVSFLTKATRLTWKSFFKAMESGARQSVGVGIACAVVG
                     NIIGITNLTGLGLVLGDNIVSLAGGSVLITTFLTMIVCVILGMGLPTTACYIVTATIA
                     APALMKLGVNPLAAHMFTYYFACLSNLTPPVAIASYGAAGLSGQSPSAVGWTGFRISL
                     AGFLIPFTFIFNPELLLVGGDYVSITISTITSIIGVIALASSLQNFLYVRMNLIQRAG
                     MFFGALLLIFPGLTTDIAGVALTGTVFFWQRMQARSRAV"
BASE COUNT          406 a          486 c          507 g          281 t
ORIGIN      
        1 gttttcattt cgtacactct cctctctccg cgcctcagac ggcacgcgaa cgagcctgca
       61 tcctctgcca gaagaaaact gttcctgtca gcgctactcc ggcgatatcc gtggtcaatc
      121 ccgggaagat aagcagcaac gcgccgaaga acatacccgc ccgttggatc aaattcatcc
      181 ggacatacag gaaattctgc agcgacgaag cgagcgcgat aaccccgatg atgctcgtaa
      241 tcgtcgagat ggtgatggag acataatcgc cccccacgag cagaagttca gggttgaaga
      301 tgaacgtgaa ggggataaga aaaccggcaa gcgaaattct gaaacccgtc cagcccacgg
      361 cggaggggct ctgccccgaa agtcccgcgg ccccgtagga ggcgatggcc accggaggcg
      421 tcaggttcga gagacaggcg aagtagtagg tgaacatgtg cgcagccaga ggattgacgc
      481 ccagcttcat cagcgcggga gccgctatgg tcgcggtaac gatgtagcac gcggtggtcg
      541 gcaaccccat gccgaggatg acgcagacga tcatcgtgag gaaggtcgtg atgaggacgc
      601 tgccgcccgc gagcgagacg atgttgtcgc cgagcacaag ccccagcccc gtcaggttcg
      661 taatgccgat gatattgcca acgacggcgc aggcgatacc gacgccgacg ctctgcctgg
      721 ctccggattc catcgccttg aagaaggatt tccaggtaag gcgggtcgcc ttcgtcagga
      781 agctgacggc gatggaggag ataatgccgt aaaacgctgc gtagagcggc gtccgtccga
      841 tgagcaggag gtagacgatc actatgatgg ggacgagaag gtgcccccgc tccctcatga
      901 ccttcgtcag gggaatgatc tcatctctgg ggataggctt catccccatc ttgacggcct
      961 cgatatggat gacggcgtac tcggccagat agtagagcac cgccggaatg gcggctgcga
     1021 gcatgatgac gccgtagtgc agccccaaga actccgccat gatgaacgac gcagccccca
     1081 tgaccggagg catgatcatt ccgcccgtcc cggcgaccgc cacaactgcc gcggagaaga
     1141 ccggcttgaa cccggccccg gtcatcagag ggatggtgaa cgtccccgtg gcggcaacgt
     1201 tccccaccgc gctgccgttg atcattccca taaggccgct ggctacgatg gagacctttg
     1261 cctcgccgcc gatggtgcgg ccggcaagcg acatcgccag atccttgatg aaacggccca
     1321 ttcccgtctc ggtcaggaaa gcgccgaaga gaatgaacag gaaaatatac gttgccgaca
     1381 cgccgaggga gatgccgaag ataccctcgg aagaaagaaa cagctggtag acgatccgtt
     1441 tgtaggtgaa ccccgtgtgt ccaagaacgc cggggatgta ttcgccccag tagccgtacg
     1501 cgagaaagag cagcgcgagg atcatcagct ccttgcccac ggaacgcctt gtcgcctcga
     1561 ggacgcagac gatgaggatc gctcccatcg tgatatccag agggatggcg gttccacgca
     1621 tactgaaggc ctcgaatgta aagaaaatat agagactcga tgaaaccgag agaataatcc
//
LOCUS       DAOPKU010000644         1673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_86034_length_1673_cov_3.474660, whole genome shotgun sequence.
ACCESSION   DAOPKU010000644 DAOPKU010000000
VERSION     DAOPKU010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86034_length_1673_cov_3.474660"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PK849_14525"
     CDS             complement(<1..1036)
                     /locus_tag="PK849_14525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPE67395.1"
                     /translation="MSIWRRSMLFNSYVFLFLFLPATWFSFRLACSRRTTNAALAVLL
                     VASMLFYSYWNPPFILLILFSILFNYAWGRGIERAVLQGGNVKGWLFSGLSVNLALIA
                     YFKYANFFMANLALLWGAEWLPREIFLPLGISFFTFQQMAYLVDCSKGLVGGHSFTHY
                     ALFVSFFPQLIAGPIVRYDQIIPQFSRLRTFALSYRNIAIGLTLLSLGLFKKLVVADT
                     LAPWAAAVFDAPGVPPILEAWVGVLCYTFQIYFDFSGYSDMALGLGRLFNIELPVNFA
                     SPYKACSMAEFWRRWHISLSTFLRDYLYIPLGGNRKGTLRRYGNLMTTMLLGGLWHGA
                     SWNFVIWGGLH"
     gene            1224..>1673
                     /locus_tag="PK849_14530"
     CDS             1224..>1673
                     /locus_tag="PK849_14530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE67396.1"
                     /translation="MKILQILPELHEGGVERHVLWLSNGLAAGGHEVVVVSAGGKMER
                     ELRGVEHVRLPVHRKNPVTAAWAALRIARIAKARGVQLLHAHSRVPAWIAWWASRLSG
                     IPWVATCHAFYSRNAGILPYRRALLLLCVSDAVRSYFAEIFPDAEFRV"
BASE COUNT          375 a          458 c          536 g          304 t
ORIGIN      
        1 cgtgcagccc tccccaaatg acgaagttcc acgaagcgcc gtgccagagc cccccgagca
       61 gcatcgtcgt catcaggttg ccgtagcgcc ggagcgttcc cttccggttc ccccccagcg
      121 ggatatacag gtagtccctc agaaaggtcg agagcgagat gtgccatctg cgccagaatt
      181 cggccatcga gcacgccttg tagggcgagg cgaagttgac ggggagttcg atattgaaca
      241 gcctcccgag cccgagcgcc atatccgagt agcccgagaa gtcgaagtag atctggaacg
      301 tgtagcagag caccccgacc caggcctcca agatcggagg aacaccgggc gcgtcgaaga
      361 cggccgccgc ccacggcgca agcgtgtccg cgacgaccag cttcttgaag agcccgagcg
      421 agagcagcgt gagtcctatc gcgatgttcc ggtagctcag cgcaaacgtg cggagccggg
      481 agaactgcgg aatgatctga tcgtagcgga cgatcggccc ggcgatgagc tgcgggaaga
      541 aggagacgaa aagggcgtag tgcgtgaagg agtgtcctcc aaccagcccc ttcgagcagt
      601 cgacaaggta ggccatctgc tgaaaggtga aaaaggagat gcccaggggg agaaaaattt
      661 cgcggggcag ccactcggcg ccccaaagga gcgccaggtt ggccatgaag aagttcgcgt
      721 acttgaaata ggcgatcagc gccaggttga cgctcaaacc gctgaagagc catcccttta
      781 cattcccgcc ttgtaatacg gcccgttcaa tgccgcgtcc ccaggcgtaa ttgaagagga
      841 tcgaaaagag aatgaggagg atgaacggag ggttccagta cgaataaaag agcatcgacg
      901 ccacaaggag cacggccaaa gcggcgttcg tcgttctcct ggagcatgcg aggcggaaag
      961 aaaaccaggt cgcgggcagg aagagaaaca gaaaaacgta ggaattaaag agcatgcttc
     1021 ttctccatat cgacatgcgg cccgttttcc cgattctgtc ctggagagcc gcaccggatg
     1081 ttgagtatta tatccgatac tttccgattt gtaattccgc gttccgatcc ttttggacaa
     1141 cgagagggaa atatgatagg cttccatcgg aaaaaccgga gcggtaccgg tgttttcggg
     1201 agcgtggaaa ggcggcggtc ctcttgaaga ttctccagat tcttcccgaa ctgcatgaag
     1261 gcggcgtcga gcgacacgtg ctgtggctct ccaacggcct tgcagccggg gggcatgagg
     1321 ttgtcgttgt ctcggccggt ggaaagatgg agagggagct gcggggcgta gagcatgtcc
     1381 gccttccggt gcacaggaaa aacccggtaa cggcggcgtg ggcggctctc cgtatagcgc
     1441 ggatcgcaaa ggcgcgcgga gtacagcttc tgcatgccca ctcccgcgtc ccggcgtgga
     1501 tcgcgtggtg ggcgtcccgc ctttccggaa taccgtgggt tgctacgtgc catgcctttt
     1561 acagcaggaa cgcgggaatc cttccgtacc ggagggcgct tctgctgctc tgcgtcagcg
     1621 acgcggtgcg ttcgtatttt gcggagatct tccccgatgc ggagtttcgg gtg
//
LOCUS       DAOPKU010000645         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_86268_length_1670_cov_4.390093, whole genome shotgun sequence.
ACCESSION   DAOPKU010000645 DAOPKU010000000
VERSION     DAOPKU010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86268_length_1670_cov_4.390093"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PK849_14535"
     CDS             complement(<1..704)
                     /locus_tag="PK849_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPE67397.1"
                     /translation="MNGLLYEKRGHVGIIRFNRPKVLNAMDTETTGEFSGLLREIEAD
                     GDIRCVVLTGEGRAFCAGGDIDEESGKDVRASFEFAVMGDRLLERMERLRVPVIAAVN
                     GYALGGGLEFVLACDVRIAADTAKLGSPEVTLGTFPGWGGTQRLPRTVGLSKAKELMM
                     TGDKIGAAEALRIGLVDRVVSAEALMDEAMALAEKIADNPPLAVKYIKISVNEGTQCD
                     LTRGIQLEANIFAQLYA"
     gene            complement(760..>1670)
                     /locus_tag="PK849_14540"
     CDS             complement(760..>1670)
                     /locus_tag="PK849_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPE67398.1"
                     /translation="MGKVEPAMAITMLCTSLAMKCVLVGGTPEQIRRVADILMPGGFG
                     AFCLTEPDSGSDAASLRTSVVRDGDEYVINGSKCFATNGQYADVYIVFATLDRKLGPK
                     GICAFIVEKGAPGLTVGRHENKMGLRLSNTCDVTFQDVRVPASSLLGKEGEGFKIAME
                     GLDEGRAYNGSIAVGICQAAVDESVKYAKERKTFGVPIIRHQAVAMMLADMEIYTEAA
                     RQLACSAMSMADQGLKITKEASICKCFCADAAMKVTTDAVQILGGYGYSREYPVEKMM
                     RDAKIFQIFEGTNQIQRLVVLREMSR"
BASE COUNT          273 a          575 c          494 g          328 t
ORIGIN      
        1 gcgtagagct gagcgaaaat attcgcctcg agctgaatcc cgcgcgtgag gtcgcactgc
       61 gtgccctcgt tgaccgagat cttgatgtac ttcaccgcca gcggcgggtt atccgcgatc
      121 ttctccgcca gggccatcgc ttcgtccatc agcgcctcgg cgctcaccac cctgtccacg
      181 aggccgatcc ggagggcttc cgcggcgccg atcttatcgc cggtcatcat gagttccttc
      241 gccttggaga gccccaccgt ccgcggcagg cgctgggtgc cgccccagcc ggggaaggtg
      301 ccgagcgtca cctccggcga accgagtttg gcggtgtccg cggcgatccg gacatcgcac
      361 gcgaggacga attccagccc gccgcccagc gcgtaaccgt tgaccgcggc gatgaccggc
      421 acgcgcaggc gctccatccg ctcgagcagg cggtcgccca tcacggcaaa ttcgaacgac
      481 gcccgcacgt ctttcccgct ctcctcgtcg atgtcgcccc cggcgcagaa agcccggcct
      541 tcacccgtca gaacgacgca gcggatatcc ccgtcggcct cgatctcacg caatagcccg
      601 gaaaattcgc cggtcgtctc cgtgtccatg gcgtttagca ctttggggcg gttgaacctg
      661 atgattccga catgccctct cttttcgtac agcagtccgt tcacattgtc ccctcttttc
      721 tacacgaaat acgaactgtg cggcacgctc cggcgaaggt cacctcgaca tctcccgcag
      781 aacgacgagc cgctggatct gattggtgcc ttcaaagatc tggaatattt tcgcgtcgcg
      841 catcatcttc tcgaccggat attcccggct gtagccgtat ccgcccagga tctggacggc
      901 gtcggtcgtc accttcatcg ccgcgtccgc gcagaagcac ttgcagatgg aggcctcctt
      961 cgtgatcttg agaccctggt ccgccatgct catggcgctg caggcaagct ggcgggccgc
     1021 ctcggtgtag atttccatgt cggcgagcat catggcgacc gcctggtgcc ggatgatggg
     1081 aacgccgaag gtcttgcgct ccttggcgta tttgacactc tcgtccaccg cggcctggca
     1141 gatgcccacg gcgatggagc cgttgtaggc ccggccctcg tcgaggccct ccatggcgat
     1201 cttgaagcct tcgccctctt tccccagaag gctggaagcg gggacgcgca cgtcctggaa
     1261 ggtcacgtca caggtgttgg agagacgaag ccccatcttg ttttcgtgtc tgccgaccgt
     1321 gagccccggc gcgccctttt ccacgatgaa ggcgcagatc cccttgggtc ccagcttccg
     1381 gtccagcgtg gcgaagacga tgtagacgtc ggcatactgc ccgtttgtgg caaagcactt
     1441 gctgccgttg atgacgtatt cgtccccgtc cctgaccacg ctcgtccgga gggacgcggc
     1501 gtccgagccg gaatccggct cggtcaggca gaaggcgccg aagccgcccg gcatcagaat
     1561 gtcggccacc ctccggatct gctcgggggt gccgcccacc agcacgcact tcatcgccag
     1621 ggaggtgcag agcatggtga tcgccatcgc gggctccacc ttgcccatct
//
LOCUS       DAOPKU010000646         1668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_86473_length_1668_cov_2.611903, whole genome shotgun sequence.
ACCESSION   DAOPKU010000646 DAOPKU010000000
VERSION     DAOPKU010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86473_length_1668_cov_2.611903"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1668)
                     /locus_tag="PK849_14545"
     CDS             complement(<1..>1668)
                     /locus_tag="PK849_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPE67399.1"
                     /translation="FSEPRNWYLRWMLKCFGENNPLILSQGADFWRTVLGVLSSEPSG
                     ILRRLSWPRGRGNEEPVWALEPSALRVTSGVAEFRCSKCGASFFAGADGAEDWNGLPC
                     PRESCDGGLAENSISGEYYGRLYRTGDVCRLFPAEHTGMLPKGRRTLLEAAFKQREGR
                     KPWDPNLLSCTPTLEMGIDIGDLSTLMLCSVPPDTASFRQRVGRAGRRDGNSLAFTVA
                     NGRPHDLYFFGEPLEMIVGDVEPPGVFLGASAVLERQFAAFCIDCWVRSGITAKDLPE
                     KASTVLNQLDGSEKNCFPHSFLMFIDRERSPLLDRFFALFGAEISDDVKRHIEQYTSS
                     SEEGIAFSLPEGLRRMQKQRASLQRKAKALRKALGKLKEDPAAKPEEIEALSREKRAL
                     ERLFRDLGELPLFNLLTDEGLIPNYAFPESGVVLRSVIYRRLEDGKLESDIFKYERAA
                     GAALRELAPSSTFYAGGRKVKIEQVDMSLSEVEEWRFCDVCSYMERVGAEERAGKCPR
                     CGSSGFGDLGQRRKLLRMKQVYAFGDDKSTLIDDDSDEREPAFYVDQ"
BASE COUNT          318 a          568 c          469 g          313 t
ORIGIN      
        1 tctgatcaac gtagaaggcc ggctcgcgct cgtcgctgtc gtcgtcgatc agggtgctct
       61 tatcgtcccc gaaagcgtag acctgcttca ttcgaagcag ctttcttctc tgcccgagat
      121 cgccgaatcc ggaactgccg caccggggac acttccccgc acgttcctcc gcacccactc
      181 tctccatgta cgagcagacg tcgcagaaac gccactcctc cacctccgaa agcgacatat
      241 ccacctgctc gatcttcacc ttgcgccctc cggcgtaaaa ggtgctcgaa ggcgccagct
      301 cccgaagggc ggcgcccgcc gcacgctcgt atttgaaaat atcgctctcg agttttccgt
      361 cctccagtct cctgtagatg acggagcgga gcacgacgcc gctctcggga aaggcgtagt
      421 tcggtatcag cccctcgtcc gtgagcaggt tgaaaagagg aagctcgccg aggtcgcgga
      481 agagccgttc aagggcgcgt ttctccctcg acaacgcctc gatctcctcc ggcttcgcgg
      541 caggatcctc cttcagcttc cccagcgctt tccgcaacgc cttcgccttg cgctgcaggg
      601 aggccctctg cttctgcatc ctccggagcc cctccggcag gctgaaggcg atcccttctt
      661 cagaagacga ggtgtactgc tcgatatggc gcttcacgtc gtctgaaatc tccgccccga
      721 aaagtgcaaa gaagcggtcc aagagcggcg accgctccct gtcgataaac atcaggaagc
      781 tgtgcggaaa gcagttcttt tcgctgccgt cgagctggtt gagcaccgtc gaggcctttt
      841 cggggaggtc cttcgccgta atccccgatc tgacccagca atcgatgcag aacgccgcga
      901 actggcgttc aagcacggcg gacgcgccca gaaagactcc cggcggctcc acgtcgccca
      961 cgatcatttc caggggctcg ccgaagaaat agaggtcgtg ggggcggccg ttggccaccg
     1021 tgaaggcgag ggagttcccg tcccgtcttc ccgccctgcc cacccgctgc cggaacgagg
     1081 ccgtatctgg cgggacggaa cacagcatca gcgtggacag gtcgccgatg tcgatgccca
     1141 tctccagcgt cggcgtgcat gaaagaaggt tcggatccca cggcttcctc ccctcccgct
     1201 gcttgaacgc ggcttccagc agagtccgcc gccccttggg cagcatcccg gtgtgctccg
     1261 ccggaaaaag cctgcacacg tctcccgtgc ggtagagcct tccgtaatac tccccggata
     1321 tggagttttc ggcgagtccg ccgtcgcagg attcccgcgg acagggaagg ccgttccagt
     1381 cctccgcgcc gtccgctccc gcaaaaaagg aagcgccgca cttcgaacac cggaattcgg
     1441 ccacgccgct ggtcacgcgg agcgccgaag gttcaagagc ccagacaggt tcttcgtttc
     1501 ctcttcctct tggccaagaa agcctccgca gaatgccgga aggttcggac gagagaacgc
     1561 cgaggaccgt cctccagaaa tcggccccct gagaaagaat cagtggattg ttctccccga
     1621 agcatttcag catccaccgg aggtaccagt tcctgggctc cgaaaagg
//
LOCUS       DAOPKU010000647         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_86579_length_1666_cov_3.901304, whole genome shotgun sequence.
ACCESSION   DAOPKU010000647 DAOPKU010000000
VERSION     DAOPKU010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86579_length_1666_cov_3.901304"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PK849_14550"
     CDS             complement(<1..1057)
                     /locus_tag="PK849_14550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPE67400.1"
                     /translation="MLFWILLLAGALGFLGYRVVAPSGAQQRIPPPSAGIMVTLYEVQ
                     PRLWEIWRSFFGTVRSAQVQKVTSQTREVIEEINVEIGDSVKKGDVLISLAGQSQVAL
                     LSARQAAYDDAKSRYDRLQSLFQAGGTSKQELDKANVSVQEEAAKLRDARTTVSRIQI
                     RSALNGIVVRRNAERGEFADPGRELLAIADFDRREVEMLISPTIRGLIRPGMDVQVKS
                     PWGGQTMGKIFRMDPEADSATGFFRGIVRLPPECTLIPGDYVRMEVRMVHKENTLAVP
                     YECILREDGQPYVFIVDGEKAVRRNILAGEGSGGYVEISSGLAPGDKVVKIGAGSLYE
                     GAKVLVSNGLLRTAAGSG"
     gene            complement(1225..>1666)
                     /locus_tag="PK849_14555"
     CDS             complement(1225..>1666)
                     /locus_tag="PK849_14555"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPE67401.1"
                     /translation="ALSLLGENILVVLGILPPVFILLGLFDVWIPRERVAPHMGEDSG
                     LKGTVLSVLLGACSAGPLYIAFPVAEVMLRKGTSLRNVFVFIGAWSTMRIPMILFEIQ
                     NLGAVFGLTRYFVSLVGILLMALLMDRVLNTAEKTEIIARFQEE"
BASE COUNT          385 a          511 c          424 g          346 t
ORIGIN      
        1 ctccggaacc tgcggctgtt cgcaataaac cgttcgagac gaggaccttc gccccctcgt
       61 agagacttcc ggcgcctatt ttcacaacct tgtcgcccgg ggcgaggcca gaggaaattt
      121 cgacatagcc tccgctcccc tctccagcga ggatattccg gcgaaccgcc ttttcgccgt
      181 cgacgataaa gacatacggc tgcccatcct cgcggagaat gcattcatag gggacggcaa
      241 gcgtattctc tttgtgcacc atcctgactt ccattctgac gtaatcgcct gggatgagag
      301 tacactccgg cggaagacga acaatccccc tgaagaatcc cgtcgccgaa tccgcctcgg
      361 gatccatccg aaagattttc cccatggtct gtccgcccca gggagacttc acctgcacgt
      421 ccatacccgg gcgaatgagc cctcgtatgg tgggagagat cagcatctcc acctcccggc
      481 ggtcgaagtc ggcgatcgcc aacagctcgc gccccgggtc cgcgaattcc ccccgctcgg
      541 cattcctccg aacgacgatt ccgttgagag ctgaacggat ttgaatacgg gagacagtcg
      601 tcctggcgtc ccgaagcttg gcggcttctt cctggacgga gacgttcgcc ttgtccagtt
      661 cctgctttga tgttccgccc gcctgaaaga gactctggag acgatcataa cgggatttag
      721 catcgtcata ggctgcctga cgagcgctca aaagcgccac ctggctctgt ccggcaaggg
      781 agatgagcac atctcccttc ttcacgctgt cgcctatctc gacgttgatc tcctcaataa
      841 cctcgcgcgt ctgagatgtc accttctgta cctgcgcgga gcgcaccgtc ccgaaaaagc
      901 tgcgccagat ttcccagaga cgcggctgga cttcgtaaag ggtcaccatg attccagcgg
      961 aagggggcgg tattcgctgc tgcgccccgg aaggcgcaac aaccctatac cctaggaaac
     1021 cgagggctcc ggcaagaagc agaatccaga acaacacatt tgctttcctc aataaaacca
     1081 tggcgtcccc cttgcggttg caaagcgata acgaatcttt gctctgtcta ccctttccca
     1141 taatatcttt attgtacatc cggagctttc tttttcgcta tgcgcaatta cgtaaaactc
     1201 gttccctata aggaacgaaa gcgcttactc ctcctgaaaa cgggctatga tctccgtctt
     1261 ctctgccgtg ttgagcactc tgtccataag aagcgccatg agaaggatgc cgacaagcga
     1321 gacgaaatac cgggtgagtc cgaagaccgc cccaagattc tggatctcga aaagaatcat
     1381 cgggatgcgc atcgtagacc atgcgccgat gaagacgaag acgttccgaa ggctcgttcc
     1441 ttttcgcagc atcacttcgg caacgggaaa ggcaatatag agagggcctg cggagcatgc
     1501 cccgaggagc acggagagca ccgtcccctt tagtcctgag tcttcgccca tatgcggagc
     1561 cacccgctcc cttggaatcc atacatcgaa aaggcccaaa aggatgaaga caggtggcaa
     1621 aatgcccaga acaacgagaa tattctctcc gagaagtgag agcgcc
//
LOCUS       DAOPKU010000648         1657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_87287_length_1657_cov_2.463171, whole genome shotgun sequence.
ACCESSION   DAOPKU010000648 DAOPKU010000000
VERSION     DAOPKU010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87287_length_1657_cov_2.463171"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK849_14560"
     CDS             complement(<1..233)
                     /locus_tag="PK849_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67402.1"
                     /translation="MKESSVDLYFFSGTGNTLLAARAVAEGLRAGGKSVRLLRLEKGI
                     SAPAGDTALGVAVTTACFSTYPFVWEALEQLPSG"
     gene            526..1083
                     /locus_tag="PK849_14565"
                     /pseudo
     CDS             526..1083
                     /locus_tag="PK849_14565"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            complement(1094..1522)
                     /locus_tag="PK849_14570"
     CDS             complement(1094..1522)
                     /locus_tag="PK849_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPE67403.1"
                     /translation="MEPAALLRAREIRPTPLRKEALALLFAAGVPLSLKTLSERFQGK
                     PPDRVSLYRTLTLLELKGIVHKVLGADGAWRYCAHPPEESGCPGNHAHFLCLECGSMF
                     CLQDQPIPVLTLPDGCVVEGKQLLAYGLCKECAELRTKGK"
BASE COUNT          366 a          432 c          485 g          374 t
ORIGIN      
        1 cctgaaggaa gctgctccag agcctcccag acaaacggat aggtcgagaa acatgcggtc
       61 gttacagcga cgccgagcgc cgtatccccc gccggcgccg atatcccctt ttcgaggcgg
      121 agcagacgga ctgatttccc ccctgcgcga agcccttcgg ctaccgctcg tgcagcgaga
      181 agtgtgtttc ctgtaccaga gaaaaaatac agatccacgg atgattcttt cattgtgtct
      241 cctttcttct ttccttacgg agagtgatga tgaagtaatt ctatcacttc cgccataatg
      301 ctgatctttt acagggtaca atctgagcta aagtagtgta tcatagtgga tgaaacaaag
      361 aatttggagg tgagagagat gagaatattg gagtcgttgc ttctttttgc gctgttcttc
      421 tccgcggaga aggcgtacgc tgcttttcgt cctcctctgg atgaaagcgc tccggaggtt
      481 tttcagacag cgtcctttgc tcttggctga ttttggggac cggatgcccg gttcgggctt
      541 cttgaaggcg tgatccgtac ccgcgtcggc tatgcgggag gaacgacgcc ggaccctacc
      601 tacgaagaga taggagatca ttcggagaca attcagatcg attacgatcc cgaagcgctc
      661 tcgtacgaag agttgctgag tgtcttctgg agcagtcata acccgtcggc tccttcctgg
      721 tcgacgcagt accgttccgc catctttgta gcaaacgaag agcagcgtcg tgcagcggag
      781 gaaagccgtg aaaaggtgaa aacgtctcgc gggggtggag tgcagacttc catagagccg
      841 ctgaagcgct tctatccggc ggaagactat caccagaagt actacctgcg gcaggagcac
      901 gtgttgatga cggatttcat gaataactac gccacttcgg aggattttat gaactctccc
      961 gcggcggcga aggtgaacgg cttcctttcc gggactggtt cagaggagta tttaaaaaaa
     1021 atactccccg gtcttggtct ttcggaagaa ggggagaaga gactcaggct tcgcgtccgt
     1081 tgaacaggag cgtttatttc ccctttgtgc gaagttctgc gcactccttg cagagaccgt
     1141 aggcgagcag ctgtttgccc tctaccacgc agccgtccgg tagcgtaagc acaggaatgg
     1201 gctggtcttg aaggcagaac atggagccgc attccaggca gagaaagtgc gcgtgatttc
     1261 cggggcagcc gctctcttcc ggagggtgcg cacagtatcg ccaagccccg tccgctccaa
     1321 ggactttgtg gacgattcct ttcagctcaa ggagcgtcaa tgtcctgtac aacgaaaccc
     1381 tgtcgggcgg cttcccctgg aagcgctcgg acagggtttt gagagaaaga gggactcccg
     1441 ctgcgaaaag aagcgcgagc gcttcttttc gcagcggcgt tggacgaatt tctcgtgccc
     1501 tcaggagcgc ggcgggttcc attacctcgc ggcaaaggcg cacacgggat agttgcatgt
     1561 acggcacttt ctgcaaagac cgcctgcgcc ccatcggcgg acttcggaga gcgtggggat
     1621 gagccccgcg aagagacggt tgagaaggat atccatg
//
LOCUS       DAOPKU010000649         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_87523_length_1654_cov_3.030644, whole genome shotgun sequence.
ACCESSION   DAOPKU010000649 DAOPKU010000000
VERSION     DAOPKU010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87523_length_1654_cov_3.030644"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PK849_14575"
     CDS             <1..400
                     /locus_tag="PK849_14575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE67404.1"
                     /translation="PKAADTLGVNVLRIKYLAVITSGVLAGFGGAALSVAIVSRFSST
                     LVSGQGFIALAAMIFGKWKPVGTLAACLLFSSAQGLTVYLGRGDLQRMLPIPSQLLAT
                     IPFLLTLAVLIGFVGKSRPPAADGIPYEKE"
     gene            complement(470..1195)
                     /locus_tag="PK849_14580"
     CDS             complement(470..1195)
                     /locus_tag="PK849_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67405.1"
                     /translation="MGKKGILFLVWFLGFVSAVEGAATWYNAYTGGMWNNPGSALIDT
                     MLYNRMMAETSLKKMQRTPSGGVGASGQVRTLYRPSSSQSSIPGKVAAVLSNDNATKK
                     ELTVFFKEGLQSFEQFAQQAGRPYDLGFSMAFFAGVCIGLTTENEVDDDAILAAASQM
                     DKLLAEQSGIAKASDTQRREFAEVLACMAVFALAGHTQAEEAGDDEAVESFKQFARQS
                     MIEILGMDTNRLRMDGRGIRIIQ"
BASE COUNT          371 a          467 c          395 g          421 t
ORIGIN      
        1 ccccaaagcg gcggatacct tgggtgtaaa tgttctcaga atcaagtacc ttgctgttat
       61 cacttccgga gttcttgcag gattcggagg ggccgcgctc agcgtggcta tcgtctctcg
      121 cttcagctcg acgctcgtga gcggtcaggg gttcatcgcc ctcgcggcga tgatcttcgg
      181 aaagtggaaa cccgtcggaa cgctcgcggc atgccttctc ttcagctcgg cccaggggct
      241 caccgtttac ctgggacgtg gggacctcca gaggatgctc cctataccct cccagcttct
      301 ggcgacaatt ccgttcctgc tcacgcttgc cgtgctgata ggattcgtcg gcaagtcccg
      361 ccctccggcg gcggacggca tcccttacga gaaagaataa cgaaaagagc ccgtcgaaaa
      421 gtttaatatg ttccgccggg ctcttttttc tagtttcatt gatggtctat tactggataa
      481 tgcgtatacc ccgaccgtcc atacgaagac ggtttgtatc cattcccaga atctcgatca
      541 tcgactgacg ggcaaactgc ttgaacgatt ctacagcctc gtcgtcgcct gcctcctcgg
      601 cctgcgtatg accggcaaga gcaaaaacag ccatgcacgc caggacctct gcgaattccc
      661 ttcgctgcgt atcggaggct ttggctatcc ccgactgttc cgcaagcagc ttgtccatct
      721 gcgacgcggc tgctagaatg gcgtcatcgt ccacttcatt ttccgttgtt aacccgatgc
      781 agactccggc aaagaacgcc atcgaaaagc ccagatcata cggacggccc gcctgttgag
      841 caaactgctc aaaactctgc aacccttctt tgaaaaatac agtcagttcc ttctttgtag
      901 cgttatcgtt gctcaaaaca gcggcaacct tgccgggtat ggaggactga gaggaagaag
      961 gccggtagag cgtccgcacc tgccccgaag ctcccactcc accggaaggc gtcctttgca
     1021 tcttcttcaa cgaggtttct gccatcattc tgttatagag catcgtgtct attaacgcgg
     1081 agccggggtt gttccacatt ccccccgtat aggcgttgta ccacgtagcg gctccttcaa
     1141 ccgcggaaac aaaacccaaa aaccatacga gaaacagaat tccttttttc cccattccgt
     1201 atcgccccct tcgaaaaaga gtcgctgccc gtacgaggtc cctccttttt acgcatattc
     1261 tactcgttgc agtttcacat gagcaagagt tctttttata aaggtgaaaa gggctttttg
     1321 ccttgattgg atgccgaaga agctgttgaa aaaagtgttt ttttggcgta tggtactctt
     1381 gtttataata tacgcctccg aatgcccttt cgcatttttt taggtatcca gaatacattt
     1441 ttgactctac cctttgcgaa tgagcggaaa aagtggtgaa ttgtcgattt ctgcttctgt
     1501 gggatatcat ctcaattcag aaacggagtg attcgaaatg acgatcaaac gatgcccgaa
     1561 cggacacttc tacgatcccg acgccaataa ttcttgtccg tggtgcgctg ttcccgccga
     1621 cgacaacgag aagacgcggc gaatcaaccc tgta
//
LOCUS       DAOPKU010000650         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_87670_length_1652_cov_3.825924, whole genome shotgun sequence.
ACCESSION   DAOPKU010000650 DAOPKU010000000
VERSION     DAOPKU010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87670_length_1652_cov_3.825924"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PK849_14585"
     CDS             <1..426
                     /locus_tag="PK849_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67406.1"
                     /translation="LGNVEAPTSHGKNLGQEVRGYCDPTAYEAICNSERFRISYPTGY
                     MELNVENFFPCPVEKGKKVFRLVREHCTAIQQEELLAILLRKAKSYADKALELDGRLD
                     DATLSEKDYDDVYSQLKNVKRQHERITRNIEQLTGRKAK"
     gene            423..>1652
                     /locus_tag="PK849_14590"
     CDS             423..>1652
                     /locus_tag="PK849_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67407.1"
                     /translation="MKIKVSVGNSRMDKKWNLVEMELDEFRDRIAITKRTAETVEQYK
                     KLSKAKQDDVKDVGGFVLGVLKNGRRKKDSVVSRSALCLDMDYAEASVIDQIEMFFSF
                     RCWFYSTHKHTPEKPRFRLIVPLSRDVTPDEYMAVGRKVAEEIGIEQFDDTTYEPSRL
                     MYWPSTSSDGEFIFREIDGALLDPDAVLAKYVDWHNTAEWPVSKRQVTVVQREVKKQA
                     DPLEKPGTVGAFCRTYSVQEAIETFLTDVYKHSAMPGRFDYIPADSQAGVVIYDDKYC
                     YSHHATDPACGKLLNAFDVVRLHRFGALDAKADEDADPGKLPSFKAMQEFAVQDEGVK
                     LQLAKERQLAASREFTADADDWQSVLELDKQGRVKDTLTNLANIVRFDPNLRAIVYNE
                     FKCMVDVIGEVPWKQVKP"
BASE COUNT          411 a          461 c          456 g          324 t
ORIGIN      
        1 cttggaaatg ttgaagctcc gacttctcat ggcaaaaacc tcggtcaaga agtacgaggc
       61 tattgcgacc ccacggccta tgaagcgata tgcaattcgg agcgtttccg tatttcgtat
      121 ccgaccgggt acatggaact gaatgtcgag aatttctttc cttgcccggt cgagaaaggt
      181 aaaaaggtat tccggctcgt ccgggagcat tgcacggcaa tccaacaaga ggagcttctc
      241 gccattctgc tccgcaaagc gaagtcctat gcggacaagg ctctcgaact ggacggacga
      301 ttggatgacg cgacgctttc tgaaaaggat tatgacgacg tttactcgca gctcaagaat
      361 gtcaaacgac agcacgagcg cattactcga aatattgaac agctcacggg gaggaaagcg
      421 aaatgaagat aaaggtatca gtcggcaaca gccgcatgga taaaaagtgg aatcttgtcg
      481 agatggagct tgatgagttc cgggatcgca tcgccatcac caagcggacg gccgaaacgg
      541 tcgaacagta taaaaagctc tccaaagcaa aacaggacga cgtcaaggat gtgggcggtt
      601 tcgttctcgg cgttctcaaa aacggacgca ggaagaagga cagcgtcgtt tcccgctccg
      661 ctctgtgcct ggatatggac tatgcggaag cgagcgtcat cgaccaaatc gagatgtttt
      721 tctccttccg ctgctggttc tacagcaccc ataagcacac cccggagaaa ccccgattcc
      781 ggctcatcgt cccgctctcc cgcgacgtaa ccccggacga atacatggcg gttggcagaa
      841 aggtcgccga ggaaatcgga atcgagcagt tcgacgacac cacctacgag ccgagccgtc
      901 tcatgtactg gccttcgacc tcctcggacg gcgagtttat cttccgagag atcgacggcg
      961 cgctgcttga tcccgacgcg gttctcgcca aatacgtcga ctggcataac acggcggagt
     1021 ggcccgtgtc aaaacgccag gtaacggtgg tgcagcgcga ggtcaaaaaa caggccgacc
     1081 cgctcgaaaa gcccggtacg gtaggggcgt tttgccggac gtactctgtg caagaggcaa
     1141 ttgagacctt tctcacagat gtttacaaac acagcgccat gccgggtcgc ttcgactata
     1201 tccccgctga ttcacaggct ggcgtggtta tctacgatga taagtattgc tacagccatc
     1261 atgcaaccga ccccgcttgc ggcaagctgc tgaacgcctt tgatgttgtc cgccttcata
     1321 ggttcggcgc gctcgacgcc aaggccgacg aagacgccga ccccggcaaa ctcccgtcgt
     1381 tcaaggcgat gcaggaattc gccgttcaag acgaaggcgt gaagctgcag cttgcaaagg
     1441 agcgtcagct cgccgcctcc cgtgaattca ccgcagacgc cgacgactgg cagagcgtcc
     1501 tcgaacttga caagcagggg cgcgtaaagg atacgctcac gaaccttgcc aacatcgtcc
     1561 gctttgaccc gaatcttcgc gccatcgtct acaacgagtt caagtgcatg gtggacgtga
     1621 tcggcgaagt gccgtggaag caggtaaagc cc
//
LOCUS       DAOPKU010000651         1648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_87961_length_1648_cov_3.968613, whole genome shotgun sequence.
ACCESSION   DAOPKU010000651 DAOPKU010000000
VERSION     DAOPKU010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87961_length_1648_cov_3.968613"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PK849_14595"
     CDS             complement(<1..559)
                     /locus_tag="PK849_14595"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPE67408.1"
                     /translation="MNPVLRTLLLLAGSGAPAVRIWNAFESEALPPEAFWEEGEVLWR
                     RLGCTEAVCRMLRRLADSSWPDEEELRAKQAGVRLLTFRDKEWPDALGGQPDSPRLLY
                     VKGKWPLRGPSVAVVGTRRCSAYGMRAAFEIGRAVGGAGGVVISGGAAGIDKAAHEGC
                     LSAGGASAAVLGTGVDVAYPRGHEPL"
     gene            complement(556..>1648)
                     /locus_tag="PK849_14600"
     CDS             complement(556..>1648)
                     /locus_tag="PK849_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPE67409.1"
                     /translation="APEVSLVKGCEAYAVEDLADLFAFFRGELELPSVPEYAPQEEDV
                     RADPDFADIRGQAGARRALEIAAAGHHNILLIGSPGSGKTLLARALRGILPPLSHEEL
                     LETTLVRSTLGLPFERGVQPPFRSVHHTASTVSICGGGATLRPGEISLAHRGVLFLDE
                     FPEFRRDLLEGLRQPLEDGAITVSRAAGSALYPCRVLLVVAANPCQCGWDGDPVERCV
                     CSHLERERYRRKISGPILDRIDLHVSVPRLLPEELVAVESAGGECSADVAKRVRAARE
                     MQRERWAPHGFSCNAELPERLVKRSLALSAGVRPFLSSMAGSLRLSGRGIGRVLKVAR
                     TIADLARSESVEVPHVAEALAYRDGGVWR"
BASE COUNT          282 a          570 c          549 g          247 t
ORIGIN      
        1 agagcggttc gtggccgcga gggtaggcga cgtcgacgcc tgttccgagg acggcggcgc
       61 ttgctccgcc agcggaaagg catccttcgt gggcagcctt gtcgatcccc gccgctccgc
      121 cgctgatgac gacgccccct gcgccgccga cggcccgccc tatctcgaat gcggcgcgca
      181 taccgtacgc cgagcatcgt ctggtcccca cgacggcaac ggaagggccg cggagcggcc
      241 acttgccttt gacgtagagc agccgcgggg agtcgggctg cccgccgagc gcgtcgggcc
      301 actccttgtc gcggaaggtg agaagacgga cgccggcctg cttcgcgcgc agttcctctt
      361 cgtcgggcca ggaagagtcc gcgaggcgac gcagcatcct gcacacagct tcggtacacc
      421 caaggcgacg ccagagaact tccccctcct cccagaacgc ctctggagga agagcctccg
      481 actcgaatgc gttccagata cgaacggcag gagcgcccga gcccgcaagc agcaggagcg
      541 tccggagcac agggttcatc tccagacccc tccgtccctg tacgcgaggg cttccgcgac
      601 gtgggggact tcaacgcttt cgcttcgcgc aagatccgcg atcgtccgcg cgaccttgag
      661 cacccgtcct atgcctcttc cggagaggcg caggcttccc gccatcgaag agaggaaggg
      721 gcgcacgccg gccgaaagag caagcgagcg tttgaccagg cgttcgggaa gctcggcgtt
      781 gcaggaaaag ccgtgagggg cccaccgctc cctctgcatc tcccgcgcgg cacgcacccg
      841 cttcgcgacg tccgcgctgc attcgccgcc cgcgctctcc accgcgacga gctcttccgg
      901 caagagccgc ggtacggaga cgtgcaggtc gattctgtcg aggataggtc cggaaatctt
      961 gcggcggtag cgctccctct ccaggtggga gcagacgcat cgttccacgg gatcgccgtc
     1021 ccagccgcac tgacaggggt ttgcggcgac gacaagcagc acgcggcagg ggtagagcgc
     1081 ggatcccgca gctctgctga ccgtgatcgc cccgtcctcc agcggctgcc gcagtccttc
     1141 gagcagatcg cgccggaact cgggaaactc gtcgaggaag agcacccctc tgtgggcgag
     1201 actgatttct ccggggcgaa gggtcgcgcc gccgccgcag atggagacgg tgctcgccgt
     1261 gtgatggacc gaccggaacg gcggctgcac cccccgttca aaagggaggc cgagcgtgct
     1321 ccgtacaagg gtcgtctcca gcagctcctc gtgggagagc ggcgggagga tgccgcgaag
     1381 cgcccgtgca aggagcgtct ttccgcttcc cggcgaaccg atgagcaaga tgttatggtg
     1441 cccagcggcc gcgatctcca gcgcgcggcg cgcccccgcc tgccccctga tatcggcgaa
     1501 gtcggggtcg gcgcggacgt cctcttcttg gggcgcgtac tccggtaccg agggaagctc
     1561 caattcgccc cggaagaagg cgaagagatc ggcaagatcc tctacagcat acgcttcgca
     1621 ccccttgacg agcgatacct ccggggcg
//
LOCUS       DAOPKU010000652         1647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_88019_length_1647_cov_6.529523, whole genome shotgun sequence.
ACCESSION   DAOPKU010000652 DAOPKU010000000
VERSION     DAOPKU010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88019_length_1647_cov_6.529523"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PK849_14605"
     CDS             <1..292
                     /locus_tag="PK849_14605"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPE67410.1"
                     /translation="SKKHERTEKPQKRAGRNGNAYAEAGAGPEGRPFIGPVIRFIIDV
                     GAERPLVVEESAASARTASPGERVSLRFSPEDLRLFGNTSPEAAPMKKEATP"
     gene            289..1065
                     /locus_tag="PK849_14610"
     CDS             289..1065
                     /locus_tag="PK849_14610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPE67411.1"
                     /translation="MKFPPHHTFALSAKAADPESRYPWALKHGFALEYTPDPEQLELL
                     PAHTAEFRRRNVPVRYHTRYFGLEFGDARAEYAEAAFDAHRRTLDAMAQVGGEVLTVH
                     AGLSKGVTLNSDTVARNLRRLADHGRSLGVTVSLENLRSGPASDPENILRWASSADTA
                     ITMDLGHVVSSEQVKSGELKGEEVARAFLPRLLEVHFYGREEGRHFPLESMKSVEHIV
                     EVVLESSCRWWTVELDNTAEALATKSLLEEYLREKGLLPA"
     gene            complement(1146..1364)
                     /locus_tag="PK849_14615"
     CDS             complement(1146..1364)
                     /locus_tag="PK849_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPE67412.1"
                     /translation="MADTVIVDARGLSCPQPVLETKKALEKLSGGRVEVLVDTVTSRE
                     NVARFGGSRGWKVEKEEREDGFCVVLAK"
     gene            complement(1382..>1647)
                     /locus_tag="PK849_14620"
     CDS             complement(1382..>1647)
                     /locus_tag="PK849_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YedE-related selenium metabolism membrane
                     protein"
                     /protein_id="HPE67413.1"
                     /translation="WNFLGMVLSGLAFTLAGGCPGRQFIMSGEGDGDSAIFVLGMLVG
                     AGFAHNFSLASSPAGVTAYGMTATAIGMVFCLAVGFLFRIKMD"
BASE COUNT          318 a          566 c          510 g          253 t
ORIGIN      
        1 aagcaaaaaa cacgaaagga ccgagaaacc tcagaaaagg gcgggcagga acggcaacgc
       61 ttatgcagaa gcaggcgccg gcccagaagg caggcccttt atcggccccg tgattcgctt
      121 tataatcgac gtgggggcgg agcggccgct tgtcgtggaa gagagcgccg catccgcgcg
      181 caccgcttcc cccggagagc gcgtctcgct gcgcttctcc ccggaggacc tccggctgtt
      241 cggaaacaca tctccggaag ccgccccgat gaaaaaggag gctacgccat gaaatttccg
      301 ccgcaccaca cgttcgccct ctccgcaaag gccgcggacc ccgagagccg ctacccctgg
      361 gcgctgaagc acggcttcgc gctggagtac actcccgacc cggagcagct ggagcttctg
      421 ccggcgcaca cggccgaatt ccgccgccgc aacgtcccgg tgcgctacca tacgcgctac
      481 ttcggcctcg aattcggcga cgcgcgcgcc gagtacgcgg aagccgcctt cgacgcgcac
      541 cgccgcacgc tcgacgcgat ggcgcaggtc ggcggcgagg tgctcaccgt ccacgccgga
      601 ctctccaagg gagtgacgct gaactccgac acggttgcgc ggaacctgcg gcgtctcgcc
      661 gaccacggaa gatcgctcgg cgttaccgtc agcctggaaa atctgcgctc gggccccgcg
      721 agcgatccgg agaacatcct ccgctgggcc tcctccgccg ataccgcaat caccatggac
      781 ctggggcacg tcgtctcctc cgaacaggtg aagtcgggcg agctgaaggg ggaggaggtc
      841 gcccgcgcct tcctgccgcg cctgctggag gtgcacttct acggcaggga agaggggcgg
      901 cacttcccgt tggagagcat gaagagcgtc gaacacatcg tggaggtcgt gctggagtcg
      961 tcgtgccgct ggtggaccgt cgagctggac aacaccgccg aagcgctggc gacaaaatcg
     1021 ctgctggaag agtatctgcg ggaaaaaggt ctcctgcccg cctgacgcgg ctgcctcgac
     1081 ggacaacaaa acggcgctct ccggagtttt ctccgaagag cgccgcatgt ttttctcacg
     1141 ctgaactact tcgcgaggac gacgcagaaa ccgtcttcgc gctcctcttt ctccaccttc
     1201 cagccgcggc ttccgccgaa gcgggcgacg ttctcacgcg acgtcacggt gtccacgagc
     1261 acctccacgc gcccgccgga gagcttctcc agcgctttct tcgtctcgag gacgggctgc
     1321 gggcacgaca gtccgcgggc gtcgacgatc acagtatcgg ccattttcac attcctcctc
     1381 gtcaatccat cttgatgcgg aacaggaaac cgacggccag gcagaagacc atgccgatcg
     1441 ccgtcgccgt cattccgtac gccgtcacgc ccgcgggcga actcgcaagc gagaagttat
     1501 gcgcgaaccc cgcgccgacg agcattccga gcacgaagat cgccgagtcg ccgtcgccct
     1561 cgccggacat gatgaactgc cttccggggc agcctccggc gagcgtgaag gcgagtccgg
     1621 agaggaccat gccgaggaag ttccaca
//
LOCUS       DAOPKU010000653         1646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_88107_length_1646_cov_4.363922, whole genome shotgun sequence.
ACCESSION   DAOPKU010000653 DAOPKU010000000
VERSION     DAOPKU010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88107_length_1646_cov_4.363922"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..977)
                     /gene="proC"
                     /locus_tag="PK849_14625"
     CDS             complement(162..977)
                     /gene="proC"
                     /locus_tag="PK849_14625"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPE67414.1"
                     /translation="MTEEFRIAILGAGNIGTSIAKGLVQSGHFAPARITLTRRRVHLL
                     EDFKAQGFDVSNDNISAVNASDVVIVSVEPQLVDGVLEQIAPALDFDRHILISVVTGI
                     SLHQIEAKLGKRMRIARAMPNTAIAVRESMTCIASNGPDDKALEMAKLLFNDVGKTLR
                     IREEDMLAATALGACGIAYFLRAIRAASQGGIEVGLSAKNSLAMAVQTAKGAACLLSV
                     GGTHPESEIDKVTTPNGCTISGLNCMEHEGFSSAMIRGITVSAEKAARLYRKE"
     gene            complement(1166..>1646)
                     /locus_tag="PK849_14630"
     CDS             complement(1166..>1646)
                     /locus_tag="PK849_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HPE67415.1"
                     /translation="ALFTVRASTKKELVAMFADLQRVADAAARLTGAEVVVDGEPVYA
                     ERYSNSVMGEAFKRNMETLGEPMEYPNPKERVGSSDVGNVSLEIPTIHEYLAIADPSV
                     AGHTAEFREASLSPRGDDVVLLAAKGLALTGWDLLTDERLRKAAKAEYLEKAAPYKS"
BASE COUNT          287 a          540 c          508 g          311 t
ORIGIN      
        1 tcctcggttc aaccgacggc gtgtggctcg cctttccgct cgcggacgcg ctctcctgcc
       61 tcgtgacggt cttcttcttc cggcgggaac tgcgatacca cctgatcacc gaccccgaag
      121 ccgcctgaga gaacggggtt ttcactgtcg agaaggccgc gctactcttt ccggtagagc
      181 ctcgcggcct tctccgcgga gacggtgatg ccgcgtatca tcgcggaact gaacccctcg
      241 tgctccatgc aattcaatcc ggagatggtg cagccgttcg gcgtggtcac cttgtcgatc
      301 tcgctctccg ggtgcgtacc gcccacggag agaaggcacg ccgctccctt cgcagtctgc
      361 acggccatcg cgagggaatt cttcgcggag aggccgactt cgatgccgcc ctgcgacgcg
      421 gcgcggatcg cccgcaggaa gtaggcgatg ccgcacgcgc ccagcgcggt ggcggcgagc
      481 atgtcctcct cgcggatgcg gagcgtcttc cccacgtcgt tgaagagcag cttcgccatc
      541 tccagcgcct tgtcgtccgg accgttggag gcgatgcagg tcatcgactc ccgcaccgcg
      601 atggcggtgt tcggcatggc gcgggcgatg cgcatccgct tgccgagctt cgcctcgatc
      661 tgatggaggg aaatgcccgt gaccaccgag atgaggatgt gcctgtcgaa gtcgagcgcg
      721 ggagctatct gctccagcac gccgtcaacc agctgcggct cgacggagac gatgaccacg
      781 tccgatgcgt tcacggcgga gatgttgtcg ttcgacacat cgaacccctg cgccttgaag
      841 tcctccagca gatggacgcg ccgcctcgtc agagtgattc tggcgggagc gaaatgcccc
      901 gactgcacca gccccttcgc aatgctcgtg cctatgttcc ccgcgccaag aatggcgatc
      961 ctgaactctt cagtcatttc tgcgaacctc ccttaataaa tgcggacacc acgcacgccc
     1021 gataccatgc cgcttcgctc acgtgcatgc gagcgaagcg gcgacgggag atccggtgag
     1081 ccctgtcaaa gtatgaggga gagtatagca aacaggctca aaaaataaaa gactatttac
     1141 tccataaaac ttgaagaaac ttttgtcagc ttttatacgg cgcggccttc tccaggtact
     1201 ccgccttcgc agccttgcgg agccgctcgt cggtcagcag gtcccagccg gtgagcgcga
     1261 gccccttcgc cgcgaggagc acgacgtcgt cgcccctcgg cgacagggag gcctcgcgga
     1321 actcggccgt atgcccggcc acggaggggt ctgcgatggc gaggtactcg tggatggtcg
     1381 ggatttcgag cgagacgttc cccacgtcgg aagagccgac gcgctccttg gggttggggt
     1441 actccatcgg ctcgccgagc gtctccatgt tcctcttgaa ggcctcgccc atgacggagt
     1501 tcgaatagcg ctccgcgtac accggctctc catcgacgac gacttccgcc ccggtgaggc
     1561 gcgccgccgc gtccgccact cgctggaggt cggcgaacat ggcgacgagc tccttcttgg
     1621 tggaggcgcg gacggtgaaa agcgct
//
LOCUS       DAOPKU010000654         1637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_88830_length_1637_cov_2.800885, whole genome shotgun sequence.
ACCESSION   DAOPKU010000654 DAOPKU010000000
VERSION     DAOPKU010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88830_length_1637_cov_2.800885"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..>1637
                     /locus_tag="PK849_14635"
     CDS             84..>1637
                     /locus_tag="PK849_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPE67416.1"
                     /translation="MSVPHGFGRRASLLPFLFLLFLLLFVVHPSWTVLSRSVFVKGVP
                     GLGNLRALLERPHLFRIVWQSVLVAGGVALCATALGACIAVTVFRTRLPLRGLFRAAA
                     VLPLIIPGFVSSLSFLFLFGRNGLITYKLLKLDLAIYTWKSVFLIQVMDFATIAFLLV
                     SASLLTMDGRPEDAARTLGASETEVFRTVTLPLLRPGLLAAGILVFMRSMADFGTPLF
                     LGGRFATLASASYSQLIGSYNLEMASAMNTLLLLICLVAFLLFRRAQAAGERTRQTAE
                     RPPEPFAFPRPLAALLWGASALFSAFVLMILVSVLLAAFTRHLGANFDLTALHFQNAF
                     LRGWRGMLNTLLFASSAALLASLGGMTAAWFVVRRPGAASRALDLLATAPFAIPGTFL
                     GVGYILAFNQPPIMLAGTWALVLILTVVRELPLGLRSGVSVLSRQDGSAEDAAATLGD
                     GAFGAFFRIVLPLARPAMIATALHAFVATVQTVGALIFIVSPGTKLLSVDVFEAVYKG
                     EIGTAAALSV"
BASE COUNT          181 a          590 c          519 g          347 t
ORIGIN      
        1 cacgcccgct cagatcgcgg ccaagaatgc gcgcctcgag gagttttcgg cgcgctaccc
       61 ccatctgaac tagggggcgt ttcgtgagcg taccgcacgg cttcggaagg agggcttccc
      121 tccttccgtt tctttttctg ctctttctgc tgctcttcgt cgtccatccg tcgtggacgg
      181 tgctgagccg gagcgtcttc gtcaagggcg ttccgggcct cggcaatctt cgcgcgctgc
      241 tggagcggcc gcatctcttc cggatcgttt ggcaaagcgt cctcgtcgcg gggggcgtcg
      301 cgctctgcgc caccgcgctg ggcgcgtgca tcgcggtgac ggtcttcagg acgaggctgc
      361 cgctgcgcgg gctcttcagg gccgcggcgg tgctcccgct gatcatcccg ggattcgtct
      421 cctcgctctc cttcctcttt ctcttcggac ggaacgggct gatcacctac aagctgctga
      481 agctcgacct cgccatctac acgtggaaga gcgtcttcct gatacaggtc atggacttcg
      541 ccacgatcgc cttcctgctg gtctcggcgt cgctcctgac catggacgga cgtcctgagg
      601 acgcggcgcg cacgctcggc gcctccgaaa cggaggtttt tcgcaccgtc acgctcccgt
      661 tgctgcgccc cggcctcctt gcagcgggca tcctcgtctt tatgcgctcc atggcggact
      721 tcggcacgcc gctctttctg ggagggcgct tcgccaccct ggcgtcggcc tcctattcgc
      781 agctcatcgg ctcgtacaac ctggagatgg cctcggcgat gaacacgctg ctgctcctga
      841 tctgcctggt cgcgttcctc ctctttcgaa gggcgcaggc ggcgggggag cggacgcggc
      901 agaccgccga acgtcctccc gagccgttcg cctttccgcg ccccctcgcg gcgcttctgt
      961 ggggcgcctc cgcgctcttc tcggcgttcg tcctcatgat cctcgtctcg gtgctgctcg
     1021 ccgccttcac gcgccacctg ggcgcgaact tcgacctcac ggcgctccac tttcagaacg
     1081 cctttctgcg cggatggcgc gggatgctga acacgctgct cttcgcctcc tccgccgcgc
     1141 tgctggcgag cctcggcggc atgaccgcgg cctggttcgt cgtccggcgt ccgggagccg
     1201 cgtcccgcgc gctcgacctt ctggcgaccg cgccgttcgc cattccgggg acgtttctgg
     1261 gagtggggta cattcttgcg ttcaatcagc cgccgatcat gctcgcgggg acgtgggcgc
     1321 tcgtgctcat cctcaccgtc gtccgcgagc tgccgttggg cctccgctcc ggggtcagcg
     1381 tactgagccg ccaggacggt tcggcggagg acgccgccgc cacgctcggc gacggcgcgt
     1441 tcggggcgtt cttccggatc gtcctcccgc tcgcccgccc ggcgatgatc gccacggcgc
     1501 tccacgcgtt cgtcgccacg gtgcagaccg tcggcgcgct gatcttcatc gtctcgccgg
     1561 gaacgaagct gctctccgta gacgtcttcg aggcggtcta caagggggag atcgggaccg
     1621 cggcggcgct ttcggtc
//
LOCUS       DAOPKU010000655         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_88869_length_1636_cov_5.480708, whole genome shotgun sequence.
ACCESSION   DAOPKU010000655 DAOPKU010000000
VERSION     DAOPKU010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88869_length_1636_cov_5.480708"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PK849_14640"
     CDS             <1..1041
                     /locus_tag="PK849_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPE67417.1"
                     /translation="LIDTADPELEHVLMEQLEDVERVDYIISLHTEQDHSGAIPTVLA
                     KYPEARVVCSTKAKELLIDHIHLDESVIDAMPDGSTLSLGDRHFTFIHTPWVHWPETM
                     CAYLHEERILFSCDFFGSHLATSDMYAGESPLIYEGAKRYFAEIMMPFRSIVKSNIKK
                     VEGYAIDIIAPSHGPAFDKPEFIMNAYRDWTSDRVSNFVAIPYISMHGSTEIMVNRLL
                     DALVERGVKVQKYELTVTDIGKLAIDLVDAATIVIGTPTVHFGPHPAVFSATHLANAL
                     RPKVKYAAIIGSYGWGTKAVEQISGLIPNLKVEVLGTVMCKGLPREEDLKALDELADA
                     IAAKHAPLMQAE"
     gene            1221..1568
                     /locus_tag="PK849_14645"
     CDS             1221..1568
                     /locus_tag="PK849_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67418.1"
                     /translation="MKRIWCAAAFLFVLLAASCAFAGAQDFVLVNKTGVEIYAVYIAP
                     SDSEDWEEDVLAVETLPSGNSVKIRFAPGADVEYWDIRVEDSDGNALEWYEFNLLEIS
                     RITLLKNGEAQYE"
BASE COUNT          343 a          497 c          488 g          308 t
ORIGIN      
        1 ctcatcgaca cggcagaccc ggaactcgaa catgtcctga tggaacagct cgaggacgtg
       61 gagcgggtcg actacatcat ctcgctgcat accgagcagg accactccgg ggccatcccg
      121 acggtgctcg ccaagtaccc ggaggcccgg gtggtctgct ccacaaaggc gaaggagctg
      181 ctgatcgacc atatccacct ggatgaatcg gtgatcgacg cgatgcccga cggctccact
      241 ctctccctcg gcgacaggca cttcaccttc atccatacgc cgtgggtcca ctggccggag
      301 acgatgtgcg cctacctgca cgaggagcgc atcctcttct cgtgcgactt cttcggttcg
      361 cacctcgcca cgtcggacat gtacgccggt gagagcccgc tgatctacga gggggcgaag
      421 cgctacttcg ccgagatcat gatgcccttc cgctccatcg tgaagagcaa catcaagaag
      481 gtggaggggt acgcaatcga catcatcgcc cccagccacg gcccggcctt cgacaagccg
      541 gagtttatca tgaacgccta ccgcgactgg acctccgaca gggtgtccaa ctttgtcgcc
      601 atcccctaca tctcgatgca cgggagcacc gagatcatgg tgaaccgcct cctcgacgct
      661 cttgtggagc gcggcgtcaa ggtgcagaag tacgagctta ccgtcaccga catcggcaag
      721 ctggccatcg accttgtgga tgccgcgacc atcgtcatcg gcacgccgac cgtccacttt
      781 ggtccgcacc ccgcggtctt ctccgcgacg cacctggcga acgcgctgcg cccgaaggtg
      841 aagtacgcgg ccatcatcgg ctcgtacgga tggggcacca aggcggtgga acagatctcc
      901 ggcctgatcc ccaatctgaa ggtcgaggtg ctgggaaccg tgatgtgcaa gggacttccg
      961 cgcgaggagg acctgaaggc gctcgacgag ctggccgacg ccatcgccgc gaagcacgcc
     1021 ccgctgatgc aggcggagta gacggcagtt ttttccgagg aactgaccgg aggagaggcc
     1081 cgtttgcgga aatttctcct ccgctatatt gaacgctgaa aaaaacaggt agtgcgcgcc
     1141 gttgtcttgt gtttttgaag ccgtctgcta tcattcgcag gaaggcgctt atctcgtaaa
     1201 aagaggggca ggttgtcaca atgaaaagaa tatggtgtgc agcagcgttt ttgttcgtgc
     1261 ttctggcggc ttcgtgcgct ttcgccggag cgcaggattt cgtgctggtg aacaagacgg
     1321 gagtggagat ctacgccgtc tatatcgctc cttccgacag cgaagactgg gaagaggacg
     1381 tgctcgccgt ggaaaccctg cccagcggaa actccgtcaa aattcgcttc gcccccggag
     1441 cggacgtcga atactgggac atccgcgtgg aggacagcga cggcaacgcc ctcgagtggt
     1501 acgagttcaa tctgcttgaa atatccagga tcacgctgct gaaaaacggc gaagcacagt
     1561 acgagtaagt acgcatcgtc atctgcgcat acacaagagg ccgttcccga atccgggggc
     1621 ggcctcttgt gatgcg
//
LOCUS       DAOPKU010000656         1635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_89009_length_1635_cov_2.312025, whole genome shotgun sequence.
ACCESSION   DAOPKU010000656 DAOPKU010000000
VERSION     DAOPKU010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89009_length_1635_cov_2.312025"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PK849_14650"
     CDS             <1..519
                     /locus_tag="PK849_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67419.1"
                     /translation="KTGAEMGFEGLPHMDFLNIVIEDRSARYKKDLEHFPLSDFQLKS
                     MVQLKEAFEKNGGTLVFVLTPFWDIRLLGDDFFERTRIAARALDEALGPVRLIGSWKR
                     DAYGLEYTDFLDNSHLSLSGAKKLTASLFKNIASIRSASPIFLEERLFGKAGEYEETT
                     GTRFHGHIHEQR"
     gene            533..1147
                     /locus_tag="PK849_14655"
     CDS             533..1147
                     /locus_tag="PK849_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67420.1"
                     /translation="MFELHRRIREFAARIQWEMKGRPIPLPNALKQAILLEHARTHGI
                     RVFVETGTYEGKTLAAMVPCFDRLYSVELGEALYERACARFASVPKVRLFQGDSGKRI
                     EDILALEREGRCLFWLDAHYSRGDTARGDEETPVCTELERIGRHPVKDHVILIDDARC
                     FGADSGYPDIPQVEEMARRLFPDSTFAVADDIIRIAPRTGNDGR"
     gene            1144..>1635
                     /locus_tag="PK849_14660"
     CDS             1144..>1635
                     /locus_tag="PK849_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67421.1"
                     /translation="MIVKLTTPSASWPLALQTPGASGKWGDVQFVIDRDCAHADAWVV
                     YEKLPGRMTTVCPPSRTILITGEPPMIKSYPEAFAAQFARVLTCHAMRHGGVISEQQA
                     LPWHYGREFDQSGHERFVESYDSLVRSDPYEDKRRLLSIICSTKRKRDGHRKRHEFVS
                     SLEQ"
BASE COUNT          341 a          490 c          501 g          303 t
ORIGIN      
        1 aagacggggg cggagatggg gttcgagggg ctgccgcaca tggactttct gaacatcgtc
       61 attgaggaca ggagcgcccg gtataaaaag gacctggagc actttcccct ctccgatttc
      121 cagctgaaaa gtatggtaca gttaaaggaa gccttcgaaa agaacggcgg aacccttgta
      181 ttcgtgctca cgcctttttg ggacatacgt ctgctcggcg acgatttctt cgaacggacg
      241 cgcatcgctg cccgggctct cgacgaagcg cttggccccg tcaggctcat aggaagctgg
      301 aagcgggacg cctacgggct ggagtatacg gacttcctgg acaattcaca cctgtcgctc
      361 tccggcgcga agaagctgac cgcctcgctt ttcaagaaca tcgcatcgat ccgctcggct
      421 tcgccgatct ttttggagga gcgtctcttc ggaaaggccg gagaatacga agagacaaca
      481 ggcacgagat ttcacgggca catccatgag caaagataag gagactttcg gtatgtttga
      541 attgcatcgg cgcatccgcg agttcgcggc acgtattcaa tgggaaatga agggcaggcc
      601 gattcccctg ccgaacgccc tcaaacaggc gatacttctg gagcacgctc gtacgcacgg
      661 catccgcgtg ttcgttgaga cggggaccta cgaggggaaa acgctcgccg ccatggtccc
      721 ctgctttgac cgtctctact ccgtcgagct gggagaagct ctgtatgaac gggcttgcgc
      781 tcgtttcgcc tccgtaccca aggtgcgcct cttccagggg gacagcggca agagaattga
      841 ggacattctt gcgcttgaga gggagggacg ctgcctcttc tggctcgacg cgcactactc
      901 ccgcggagac acggcgagag gagatgagga gactcccgtc tgcacggagc tcgaacgcat
      961 cggacggcat cctgtgaaag accacgtgat tctgatcgac gacgccagat gcttcggcgc
     1021 ggactcggga tacccggaca tcccgcaggt cgaggagatg gcccggcggc tctttccgga
     1081 ttccaccttc gccgtcgcgg acgacatcat ccgcatcgct cctcgaaccg gcaacgacgg
     1141 cagatgatcg tcaaactcac caccccctcg gcgtcgtggc cgcttgccct gcagacgccg
     1201 ggtgcatcgg gaaagtgggg ggacgtacag ttcgtgatcg accgggactg cgcccacgcc
     1261 gacgcgtggg ttgtctacga aaaactcccc gggaggatga cgacggtttg cccgccgtcg
     1321 cgaacgattc tgatcaccgg cgagccgccg atgatcaaga gctacccgga ggcgttcgcg
     1381 gcgcagttcg ccagggtgct cacctgccac gcgatgcggc atggaggagt tatttccgaa
     1441 cagcaggcgc ttccgtggca ctacgggcgc gagttcgacc aatcggggca cgagcggttc
     1501 gtcgagagct acgattccct cgtccgaagc gacccctacg aagacaagcg ccgccttctc
     1561 tccatcatct gctcgacgaa gcggaagcgc gacgggcaca ggaaacggca cgagttcgtc
     1621 tcctccctgg agcaa
//
LOCUS       DAOPKU010000657         1621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_90139_length_1621_cov_2.577267, whole genome shotgun sequence.
ACCESSION   DAOPKU010000657 DAOPKU010000000
VERSION     DAOPKU010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90139_length_1621_cov_2.577267"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..>1621)
                     /locus_tag="PK849_14665"
     CDS             complement(304..>1621)
                     /locus_tag="PK849_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67422.1"
                     /translation="RIGLRRYLARDFRPNTLYLDNEYLRYANVLCAAEEQRRASSAHC
                     SEAPFSDLNPVFLHLLHNELHPVYPVPQDLYPLKQQAANGPHHAFPLRTCAEKNYVLT
                     LGYGWSGSLSYSQLLREGYPGAVYRHLAQAFRVNRIYHGPGEIVVRKSKTIARTSPKI
                     QKVVFPLFQAIHQLLRSKSFYVCESELQVAERVIGFRETIGRNLVRDETYGMESSFSQ
                     YPLNRFIRTSGVSFLLPGTPGKSGAGKYVCDAFCSVCQIREGDVDWGVFVGKGENFGV
                     GEMHGFSVYDSTYGSLRLTKLIMPRLDEILKEAMRLAEKNGASLVVETLAGMKEMLPL
                     LEERSPQIYGADTNVACEDDGWLLMVAPGEKAILHDGDSHIFEEVRVLSFVYSPKGMM
                     YTLEPRRGESRWSVPRELIRPFPDAHLVRYHLDTGETLEEEPRCSA"
BASE COUNT          388 a          451 c          387 g          395 t
ORIGIN      
        1 tgtcgtttcc aagatgacag actcagcgtg tctcccgtga ggtacacagt tgcgatagca
       61 ttaaaacgat tcggcaggca gctttagata gctcattgtt tcctttttcc gattccggcg
      121 gcggatcttg ccgcgaaggc gctttacccg tcgcctggca gatgtcttcg atgttgtgta
      181 tgtccaggaa tcaatctttc cttcgggctt cataaggaag cgcccccttt cttttatata
      241 tgaaaatcat agcataaaga ggctacaaga tgaacttttc ctttttttct tgttccgcct
      301 ctttcacgcg gaacaacgcg gctcctcctc cagcgtctcg cccgtgtcca gatgataccg
      361 cacgagatga gcgtccggaa aggggcgaat cagttcccga ggcacactcc agcgagattc
      421 ccctcttcgt ggctccagag tgtacatcat tcctttgggt gaatagacga acgaaagcac
      481 ccggacctct tcgaagatat gcgaatcccc gtcatgaaga attgcctttt caccgggagc
      541 aaccataaga agccatccgt catcttcaca tgctacatta gtgtccgcac cgtatatttg
      601 cggtgagcgt tcttcgagca acggaagcat ctccttcatt cctgcaagag tttcaactac
      661 aagagaggct ccattctttt ccgcaagacg cattgcctct ttcaatatct catccaaacg
      721 aggcataatc aattttgtga gtcgtaacga cccgtaggtt gaatcataaa cagagaaccc
      781 gtgcatctct cccacgccga aattctctcc ctttccgacg aaaacacccc agtccacatc
      841 tccttcccgg atctggcaga cggagcagaa tgcgtcgcaa acatacttgc ctgcgccgct
      901 tttccccggt gttcccggaa gaagaaagct gacgccgctc gtcctgataa agcggttcag
      961 aggatactgt gaaaacgaac tctccattcc gtaggtctcg tcccgaacca gattccgtcc
     1021 aatagtttcg cggaacccaa tcacacgttc cgccacttgc aattcgcttt cacaaacgta
     1081 aaagctcttt gaccgcagca gttgatgtat tgcctgaaaa agaggaaaca ctactttttg
     1141 aatctttggc gatgttcgtg cgatagtctt gctcttccga acgacaatct ctcccggacc
     1201 atggtatatc cgattgacac gaaatgcctg cgccagatgg cgatagaccg cgccggggta
     1261 tccttcgcgg agcaattggg aataggagag cgaaccgctc cagccgtacc cgagcgtcag
     1321 cacatagttc ttttccgcgc acgtacgaag cgggaaagca tggtgcgggc cgttcgccgc
     1381 ctgctgtttc agagggtaga gatcctgagg aaccggatag actgggtgca gctcgttatg
     1441 cagcagatgg agaaagaccg ggttcaaatc gctgaacggc gcttccgaac agtgtgcaga
     1501 agaagctcgc cgttgttctt cggcagcgca gagcacgttg gcgtagcgaa gatactcgtt
     1561 atccagatag agcgtattcg gcctgaagtc tcgcgctaaa taccttcgca aaccaatcct
     1621 g
//
LOCUS       DAOPKU010000658         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_90598_length_1615_cov_4.101923, whole genome shotgun sequence.
ACCESSION   DAOPKU010000658 DAOPKU010000000
VERSION     DAOPKU010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90598_length_1615_cov_4.101923"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PK849_14670"
     CDS             <1..291
                     /locus_tag="PK849_14670"
                     /inference="COORDINATES: protein motif:HMM:NF014856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HPE67423.1"
                     /translation="ASGGYPGTFEKGKKIEGLENVSSMEDFVLFHGGTTFSPEGKILT
                     EGGRVLSAVGLGASLEEALVNAYAGVEQISFENMHYRRDIARRAIIQRRGGI"
     gene            293..820
                     /gene="purE"
                     /locus_tag="PK849_14675"
     CDS             293..820
                     /gene="purE"
                     /locus_tag="PK849_14675"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPE67424.1"
                     /translation="MPLVSPEVGILIGSKSDYPLVKKVEDVFAFFGVSWEISVASAHR
                     TPDDVVAYVNNAPKRGIKVLVAAAGLSAALPGVVAAHTRLPVIGIPVNTGTVGGLDAL
                     LSVAQMPPGVPVASVGIDGVKNAALFAVRILALLREDLAGKLDAWSKKEEDAVRTARN
                     DLEGAHSAPGEAFTL"
     gene            complement(871..>1615)
                     /gene="argG"
                     /locus_tag="PK849_14680"
     CDS             complement(871..>1615)
                     /gene="argG"
                     /locus_tag="PK849_14680"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPE67425.1"
                     /translation="EHGVPVKATAHSPYSIDDNIWGRSIECGILEDPWNEPPSDAYTL
                     TCEPHEAPDQPEYVEILFEKGIPVALDGVRMDGPTLIDKLNCQAGKHGVGRIDMLEDR
                     LVGFKSREVYECPAAITLITAHRALETLTLDKRVLATKKELEVKFGELAYEGYWFSPL
                     KDAINSFIDTTQEYVNGVVKVRLYKGMAVVRGMKSGKSIYSETLATYSENDAFDHSAA
                     VGFIKIWGLPIKTWRQVHESKEAIEIPAQ"
BASE COUNT          346 a          423 c          457 g          389 t
ORIGIN      
        1 gcctccgggg gataccccgg gacgtttgag aaagggaaaa agatagaggg acttgagaat
       61 gtatcatcca tggaagattt tgttcttttc cacggaggca caaccttctc tcccgaggga
      121 aagattctca cagagggcgg acgcgtcctc agcgccgtcg gcctcggggc atcgctggag
      181 gaagctctcg taaacgccta tgcgggtgtg gaacaaattt cttttgaaaa tatgcactac
      241 aggagagaca ttgcccgaag ggcgattatt cagagaagag gaggcatctg atatgccgtt
      301 ggtttcacca gaagttggga tactgatcgg atcaaaatcg gattatccct tggtcaagaa
      361 agtcgaggat gtctttgctt ttttcggagt ttcttgggag atttccgttg catccgctca
      421 cagaacaccg gatgacgtcg tcgcctacgt gaacaacgcg ccgaaacgcg gaatcaaagt
      481 cctcgtggcc gccgcggggc tctcagccgc ccttccgggc gttgttgcag cgcacacgcg
      541 ccttcctgtc atcggtattc cggtgaacac cgggactgtc ggaggacttg acgcgcttct
      601 ctcggtagcg cagatgcccc ccggcgtgcc ggtggcttca gtgggcatag acggggtgaa
      661 gaatgcggcg cttttcgcgg tacgtatcct cgcgcttcta cgagaggatc ttgcgggcaa
      721 gcttgatgca tggtcaaaaa aagaggaaga cgccgtccga acggctcgaa acgatcttga
      781 gggggcgcat agcgctcccg gcgaggcgtt tactctgtaa aaaacggagg aaccgggata
      841 tcctcggttc ctccgttttt tcttgtcctt ttactgtgca gggatttcga tggcttcctt
      901 gctctcgtgg acttgcctcc acgttttaat cggcaggccc cagatcttga tgaagccgac
      961 tgccgcagag tggtcgaatg catcgttttc cgagtaagtg gcaagagtct cgctgtaaat
     1021 ggacttaccg gatttcattc cgcgcaccac ggccattccc ttatacagac ggactttgac
     1081 cacgccgttg acatactcct gcgtggtgtc gatgaaggag ttgatcgcgt cctttaacgg
     1141 ggagaaccaa tacccttcgt aggcaagttc gccgaatttg acttcgagtt ctttcttcgt
     1201 tgcaagaacg cgtttgtcaa gagtgagagt ctcaagggcg cgatgcgcgg taatcagtgt
     1261 gatagccgcc ggacactcat aaacctcgcg gctcttgaaa ccgacaagtc tgtcttcgag
     1321 catgtcgatc cgccctactc catgttttcc cgcctgacag ttcagtttat caataagcgt
     1381 cgggccgtcc atacgaacgc cgtccagagc gaccggaatt cccttctcga acagaatctc
     1441 cacatattca ggctgatccg gggcttcatg aggctcgcag gtcagtgtgt aggcgtcgga
     1501 aggaggttcg ttccacggat cctccaaaat tccgcattcg atggagcgcc cccagatatt
     1561 gtcgtcgatg ctgtagggag agtgggctgt cgcttttaca ggaactccat gttcc
//
LOCUS       DAOPKU010000659         1613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_90780_length_1613_cov_3.712452, whole genome shotgun sequence.
ACCESSION   DAOPKU010000659 DAOPKU010000000
VERSION     DAOPKU010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90780_length_1613_cov_3.712452"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK849_14685"
     CDS             complement(<1..241)
                     /locus_tag="PK849_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HPE67426.1"
                     /translation="MPPKTHALLSASSANRWLHCPPSARLNELCEDQGSDYAAEGTAA
                     HALCEFKLRKALGMEAQDPTENLSWFNEEMDDCAEG"
     gene            complement(231..548)
                     /locus_tag="PK849_14690"
     CDS             complement(231..548)
                     /locus_tag="PK849_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HPE67427.1"
                     /translation="MNEIANAINELRTAATTITRLSDLLYAELLAVDPNAGQTETPVP
                     ETAPALTLEAVRAILAEKSRDGHTAKIRALLQKYGATKLSSVDPANYKALLMDVEELT
                     NAT"
     gene            complement(541..741)
                     /locus_tag="PK849_14695"
     CDS             complement(541..741)
                     /locus_tag="PK849_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67428.1"
                     /translation="MKHNLKISVSKEPQSGGIVCCRNVTLREKILTRLLGKKERMMIL
                     VPGDSVAELCISEAAEGGGLNE"
     gene            complement(823..1203)
                     /locus_tag="PK849_14700"
     CDS             complement(823..1203)
                     /locus_tag="PK849_14700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPE67429.1"
                     /translation="MKIKYQFDNETVEIEVSEEWGSILADLDRQEYNNNHKESRRHYH
                     LDACAYEGEDFAAEDPALTALLERDDRVAKAVETLPPAQRELMKAMFWDGMSQSEYAR
                     LRAVSKATVSIAYHRAIKKLREIL"
BASE COUNT          270 a          469 c          441 g          433 t
ORIGIN      
        1 agccctcggc gcagtcgtcc atctcctcgt tgaaccagga aaggttctcc gtggggtctt
       61 gggcttccat gcccagcgct tttctgagct taaactcgca aagcgcgtgc gcggcggtgc
      121 cttccgcggc gtagtcgctg ccctgatcct cgcaaagctc gttcagcctc gccgatggcg
      181 ggcaatgcag ccagcggtta gcggaggacg cggagagcag cgcgtgtgtc ttaggtggca
      241 tttgtcagtt cctccacgtc catgagcagc gccttgtagt ttgcggggtc gacgctggat
      301 aacttggtcg ccccgtactt ctgaagcagt gcgcgaatct tggccgtgtg cccgtcgcgg
      361 gacttctccg cgaggatcgc cctgaccgct tctaatgtga gagcgggcgc cgtctccggg
      421 acaggcgttt ccgtttggcc cgcgttcggg tctaccgcaa gcagttctgc atacagaagg
      481 tcggacaagc gggtgatcgt cgtcgccgcc gtgcgcagct cgttgatggc gttggcgatt
      541 tcattcattg agaccgcctc cttccgccgc ctcagagata cacagctctg cgacgctgtc
      601 gcccgggacg aggatcatca tccgctcctt ctttcccaga aggcgcgtca ggatcttctc
      661 gcggagcgtt acattcctgc agcagacaat gccgccggat tggggttcct tggaaacact
      721 gatcttgaga ttgtgtttca ttccgttccg tcctttccga agggctcgta ttgtttcgcc
      781 cttcgctata cggccacggg aaaggtcgat agttagcctc tttcacaata tttctcgaag
      841 tttttttatc gcccgatgat acgctatgga aaccgtggct ttgctgacgg cacgcagacg
      901 cgcatactcg ctctgagaca tgccgtccca gaacatggct ttcatcaatt cccgctgcgc
      961 gggcggcagc gtttccacgg cttttgctac gcgatcgtca cgttcaagca gggccgtgag
     1021 cgcggggtct tccgcggcga aatcctcgcc ctcatacgca caggcgtcca gatggtaatg
     1081 ccgccgcgat tccttgtggt tgttgttgta ctcctgtcgg tcgaggtctg cgaggatgga
     1141 accccattcc tcggatactt cgatttcaac ggtctcgttg tcgaattggt acttgatttt
     1201 catttcgctt tcctttcttt gctctttgtg gaggcaaagc aaggagggag cgatattccc
     1261 ggaaatgaaa aaggccgggg gtatagcttt tcttgctatg cctccggcca tttggtcgct
     1321 cgttgcccga ctattgatcg ggacggcgcc gttgctcggt agtcaattgg tttggtttta
     1381 ctgtgctgcc aaatcgggaa ccggtacgct taccacgtta tgacaatgtg gacatttcat
     1441 ctgtatctca aaatcacctc gtggccactt tgacacgtca aatgcgcgtc gcccgcactt
     1501 cgggcatttc atcgccttga ttctcattgt cgttgccgcc gacgtcttta cgttcatata
     1561 cactctatcg tcctcctctc attcagcctg cttcgacttt tccattcctt tct
//
LOCUS       DAOPKU010000660         1606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_91329_length_1606_cov_3.634429, whole genome shotgun sequence.
ACCESSION   DAOPKU010000660 DAOPKU010000000
VERSION     DAOPKU010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91329_length_1606_cov_3.634429"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PK849_14705"
     CDS             <1..280
                     /locus_tag="PK849_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE67430.1"
                     /translation="EFIKDSVPAVAVEGHTDGVPLRGGIYRDNWGLSAARAAAVASYL
                     QDRAGFDPLKLQAVGYGPNRPLVPNDTPEHMALNRRVDLVFLSQYPKQ"
     gene            293..736
                     /locus_tag="PK849_14710"
     CDS             293..736
                     /locus_tag="PK849_14710"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPE67431.1"
                     /translation="MKRIILFIIMAILLFSVGFGGGIMVGRTWLNPEGSTSVTVEEPG
                     PVYFVGDFIANLSGAGNHVANFKLSLEVSSDRAMEMVSSASWVARIRNEVILLVKDRV
                     FDELTTAEGIMQLAEDIKRTINAMMPSVRDKAPVVRVLFEGFVLQ"
     gene            772..>1606
                     /gene="fliM"
                     /locus_tag="PK849_14715"
     CDS             772..>1606
                     /gene="fliM"
                     /locus_tag="PK849_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HPE67432.1"
                     /translation="MTPDVMSQSEIDSLLEALSTGSVDVDSITRDSDEKKIKSYDFRR
                     PDKFSKDQLRAIQMIHESFTRQLTSTMSTMVRSMVSAEIASVDQLAYDEFIRSLVQPT
                     VIGILEMYPFSGNALIEINPNLVFAIIDRMLGGRGEFTGKIRELTDIERTVVERLLMR
                     MLELLEEAWSTVVDVRFRYENLESNPFFVQICPGTDMVLLVILKLKVSEVEGMVSICI
                     PYFLMEPIMDKLSSQQWFASTGRKTDERSGEYIRKHIGKVRVPVALELGHTVLSVADV
                     LQ"
BASE COUNT          346 a          391 c          506 g          363 t
ORIGIN      
        1 ggagttcatc aaggactcgg ttcctgctgt ggccgtcgaa gggcatacgg acggcgtgcc
       61 cctgcggggg gggatatacc gcgataactg gggcctttcc gcggcccggg cggcggcggt
      121 cgcctcgtat cttcaagaca gggcggggtt cgacccgctg aagctgcagg cggtggggta
      181 cggtcccaac cgccctcttg tgcctaacga cacgccggag cacatggcgc tgaaccgcag
      241 agttgatctg gtctttcttt cgcaatatcc gaaacagtag gaggagaaga cgatgaaacg
      301 tattattctc tttataatta tggcgatcct tctgttctcc gtcggtttcg gaggcggtat
      361 aatggtcggg aggacgtggc tgaatccgga aggttcaacc tccgtgacgg ttgaagaacc
      421 ggggccggtc tacttcgtcg gcgatttcat cgcgaatctc tcgggcgccg ggaatcatgt
      481 ggcgaacttt aagctttcac ttgaagtaag cagcgaccgc gcgatggaga tggtgtcctc
      541 ggcaagctgg gttgcgcgga tacgcaacga agtgattctt ctcgtcaagg acagagtgtt
      601 cgacgaattg acgacagcag aggggataat gcagctcgcg gaggacatca agcgtacaat
      661 aaacgcgatg atgccctctg ttcgtgacaa ggcgcctgtg gtcagggtcc ttttcgaggg
      721 atttgtcctt cagtagcgct acgatgagaa tgcgggaagg aggcggaaat cgtgacgcct
      781 gacgtgatgt cgcaaagcga gattgactct ttattagagg cgctctccac gggaagcgtt
      841 gacgttgatt ccataacgcg cgattccgat gaaaaaaaga tcaagagtta cgacttccgc
      901 cgccccgaca agttcagcaa ggaccagttg cgggcgatcc agatgatcca cgagtcgttc
      961 acccggcagc tgacctcgac tatgtcgacg atggtgcggt cgatggtctc cgctgaaatt
     1021 gcttcggtgg accagctcgc ttacgacgag tttatccgct cccttgtgca gcctacggtg
     1081 atcggtattc ttgagatgta cccgttcagc gggaacgcgc tcattgagat caatcccaat
     1141 cttgtgttcg cgatcatcga caggatgctc ggcggccgcg gagagtttac cggcaagatc
     1201 agggagctga ccgatatcga gcggacggtt gtggagcgtc ttcttatgag gatgcttgaa
     1261 ctgctagagg aagcgtggag caccgtggtg gatgttcgct tccgttacga gaatcttgag
     1321 agcaatccgt ttttcgtcca gatctgtccc ggtacggaca tggtgctgtt ggtgattctg
     1381 aagctgaaag tgagcgaagt cgagggaatg gtgagtatct gcatcccgta tttcctgatg
     1441 gagccgatca tggacaagct gagctcgcag cagtggtttg cctcgacggg aagaaaaacc
     1501 gacgagaggt ccggggagta tatacggaaa catatcggca aggtcagagt ccccgtagcg
     1561 ttggaactgg ggcataccgt tctcagcgtg gccgatgttc tgcagc
//
LOCUS       DAOPKU010000661         1601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_91742_length_1601_cov_3.280724, whole genome shotgun sequence.
ACCESSION   DAOPKU010000661 DAOPKU010000000
VERSION     DAOPKU010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91742_length_1601_cov_3.280724"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PK849_14720"
     CDS             <1..765
                     /locus_tag="PK849_14720"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPE67433.1"
                     /translation="NLDALGGWNLFFAVYGTYFLLPLIAFGIGRLFKEDRKRLAISAL
                     TSMRSNQVFMGIPAVSIAMGNPGLEALSLFLAMSLVGYHVISIASSQLVITRELSVRS
                     LLGTISKVVRNPMVCACLVGILCSSLGFREFPGWIDTTLKVLGDIGTGLALLALGASI
                     KVVSLKGVFRSTWRDCTIKLFLHPLTLFLFFSLLPVEKTMMQVVVFCSAMPVAVNSLV
                     VAQGMGMDEHYAAELIAVSTMLSVFTLPLWIRFLGI"
     gene            complement(782..>1601)
                     /locus_tag="PK849_14725"
     CDS             complement(782..>1601)
                     /locus_tag="PK849_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE67434.1"
                     /translation="QKNEEVWLVDTGLDDEAGRKIARFLETENKKLRCIVGTHSNADH
                     CGGNAFLKKRTGCLIAATPLEAAVMENTFMEPLLLWTAFPFAEISNRFLQAKPSSVDI
                     LIRHSGVFDESGLEAVPLPGHFLEMIGVRTPDGVFFVADALFSETLLEKYHIIFVLDV
                     QAALETLTFLDKQEADWFVPSHAPAVEDIRPLAEANRRSLMRVSQAVLDSCSEPIGKD
                     EIVKRVGALFGISMSITEFLLYSAAISAHLTWLRKNALISPFVEDGLLLWRRTP"
BASE COUNT          356 a          446 c          388 g          411 t
ORIGIN      
        1 aaccttgacg ctctcggagg atggaatctt ttcttcgcgg tgtacggaac gtactttttg
       61 ctaccgctca tagccttcgg aatcggcaga ttatttaagg aggatcggaa acgtcttgca
      121 atatccgctc tgacatccat gcgttcaaat caggttttca tgggaatccc cgcagtatca
      181 atcgctatgg gaaacccggg gcttgaagcg ctgtcgcttt tcctggccat gagcctggtg
      241 gggtatcatg tcatttccat tgcttcttcc caacttgtta ttaccaggga gctttctgtg
      301 cgttctctcc tcggaacgat atcaaaagtc gtacgaaacc cgatggtatg tgcctgcctc
      361 gtcggaatac tctgctcctc cctcggcttc cgtgagttcc ccggatggat agataccaca
      421 ctgaaagtcc ttggcgatat cggaaccggt ctcgctctgc tcgccctggg agcgagtatc
      481 aaagtcgtct ccctcaaagg ggtttttcgt tccacgtgga gagactgcac aatcaagctc
      541 ttccttcatc ccttgactct tttcctcttt ttctctctcc tccccgtcga aaagacaatg
      601 atgcaggtcg ttgttttttg ctccgcaatg ccggtggccg tcaactctct ggtagtcgcc
      661 cagggaatgg gaatggacga acactacgcc gccgagctca tcgccgtgtc gacaatgctc
      721 tctgtgttca cgcttccctt gtggatccgt tttttaggga tctgagggat tacgccgcat
      781 gtcagggggt acgcctccac aagagcagcc cgtcctcaac aaacggagaa atgagcgcgt
      841 tttttcgtaa ccaggtcagg tgcgccgata tcgccgcgct gtagagaagg aactccgtga
      901 tcgacatgga gatgccgaag agagcgccta cccttttgac tatttcgtcc ttccctattg
      961 gttccgagca gctgtcaaga accgcctgac tcaccctcat caagctccgg cggttcgctt
     1021 cggccagagg tcgaatatcc tcaacggcag gggcatgact cggaacgaac cagtcagcct
     1081 cctgcttatc gaggaatgta agcgtctcaa gcgcagcctg tacgtcgagc acaaaaataa
     1141 tatgatactt ctccagaagc gtttccgaaa aaagagcatc cgcaacaaaa aacacaccgt
     1201 caggggtgcg tactccgatc atttcaagga aatgcccggg aagaggaact gcttccagtc
     1261 cgctctcgtc gaataccccg gaatgccgta taagtatatc cacagaggat ggtttcgctt
     1321 ggagaaatct gttcgatatc tcggcgaaag gaaatgcagt ccacagcagc agaggctcca
     1381 taaatgtatt ttccataacc gccgcctcca atggggtggc agctatcaaa cagcccgttc
     1441 gctttttgag aaaggcgttt cctccgcagt gatccgcatt tgagtgcgtc ccgacgatac
     1501 agcggagttt tttattttct gtctctaaaa aacgtgcgat cttccgcccc gcttcatcat
     1561 ccaagccggt atccacaagc cagacttctt cgtttttctg g
//
LOCUS       DAOPKU010000662         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_91804_length_1600_cov_6.167638, whole genome shotgun sequence.
ACCESSION   DAOPKU010000662 DAOPKU010000000
VERSION     DAOPKU010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91804_length_1600_cov_6.167638"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..532)
                     /locus_tag="PK849_14730"
     CDS             complement(77..532)
                     /locus_tag="PK849_14730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HPE67435.1"
                     /translation="MIIGTLAACERYYVLGERFRKALEFLKEHDVAAMEPGRYDIEGD
                     DVFALVQEYVTKTIDDCTLEAHRVYADVNYVAEGFEYLGYAPLERAGTPAVEYDPKTE
                     AAFFEKECDFILLRKGDIAIVFPEDAHMPQKRALVPATVRKVCIKVRVG"
     gene            complement(791..1060)
                     /locus_tag="PK849_14735"
     CDS             complement(791..1060)
                     /locus_tag="PK849_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPE67436.1"
                     /translation="MTCMRKLNLTKDFMKFCDVLPPKQFKQVVAKTLALASNPRPHDS
                     IELKGSSGQFRVDIGEYRVIYELDKEEVRVCVIDKRNDGQVYRSG"
     gene            complement(1038..1310)
                     /locus_tag="PK849_14740"
     CDS             complement(1038..1310)
                     /locus_tag="PK849_14740"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPE67437.1"
                     /translation="MPVVVNATEFKQRFGAYVEHAQKEPVEITRSGRSSAVLLSADEF
                     RRMREHEDKYWAMLAEEAKRGGFAGTEESAEWLAEGMRRCDLHEEA"
     gene            complement(1395..>1600)
                     /locus_tag="PK849_14745"
     CDS             complement(1395..>1600)
                     /locus_tag="PK849_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67438.1"
                     /translation="RRGLPFSVDIPNDVTVAASWKPAVSKIWQLVCRLPLRGLYDRLG
                     DGKNHDAVVRGETRVRTPLARSC"
BASE COUNT          340 a          484 c          427 g          349 t
ORIGIN      
        1 agagcagagt ataacacgtg taaaaaacat ttgtcagcac tgctcgtgat actgttcttt
       61 tcgctctccg ttcctttcag ccgacccgta ccttgatgca gaccttgcgg accgtcgccg
      121 ggacgagggc gcgcttctgc ggcatgtggg cgtcctcggg gaagacgatg gcgatgtcgc
      181 ccttccgcag aaggatgaag tcgcactcct tctcgaagaa tgccgcctcc gtcttgggat
      241 cgtactcgac ggcgggcgta ccggcgcgct caagcggcgc gtaccccaga tactcgaacc
      301 cctcggcgac gtagttcacg tcggcgtaca cccgatgcgc ctccagcgtg cagtcgtcga
      361 tggtcttcgt gacgtactcc tgcacgaggg cgaagacgtc gtctccctcg atgtcgtagc
      421 gccccggctc catagccgcc acgtcatgct ccttcagaaa ttcgagcgcc ttccggaagc
      481 gctcccccaa cacataatac cgttcgcaag cagccagcgt gccgatgatc atactcttcc
      541 ccgcctttcg gttgtatcga ggcgctttcg cgcccggaca gtacgatagc atgaagagga
      601 agggtgctac aagagcgggg agacgtcagt gtgggatgtt tttcgggcga catgcaatac
      661 cattagattg cgccagccct gcatagacct tgcccagatt tgcaaggaaa agaagatcgc
      721 agcggaaata tggcatggcg agcgaaatcg tcggcttgaa tcgttcttct tttcattccg
      781 caggactatc ctatccgctc ctgtacacct gtccgtcgtt ccttttgtct ataacgcaga
      841 cgcgcacttc ctccttgtcg agctcgtaga ttactcgata ttcaccgatg tcgacgcgaa
      901 actgccctga ggatcccttc agctcgatgc tgtcgtgcgg gcgagggttc gatgcgagcg
      961 ccaaagtctt cgccacgacc tgtttgaact gtttcggcgg aaggacgtcg cagaacttca
     1021 tgaagtcctt tgtcaggtta agcttcctca tgcaggtcac aacggcgcat tccctcggcc
     1081 agccactccg cggactcctc cgttccggcg aaaccgccgc gcttcgcctc ctccgcaagc
     1141 atagcccaat acttgtcttc atgctcccgc attcgacgga attcgtcggc ggagagcagc
     1201 acagccgaag aacggccgga acgagtgatc tcgacaggct ccttttgagc atgctcgaca
     1261 tacgcgccga aacgctgctt aaattccgtc gcattcacca caacgggcat tctactcacc
     1321 tcttaattat tatgattaaa acgatcataa cgatcatttc gcctctacaa ggatactctt
     1381 tcgaaggggg tacgtcaaca ggaccgagca aggggggtgc ggacacgggt ttcaccacgg
     1441 acgacagcat catgattctt gccgtcgcca aggcgatcat agaggccgcg cagaggtaac
     1501 cgacaaacga gttgccagat cttcgaaact gccggtttcc agctcgccgc gacggtgacg
     1561 tcgttgggga tatcgactga aaaaggaagc ccccggcgca
//
LOCUS       DAOPKU010000663         1597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_92053_length_1597_cov_5.034371, whole genome shotgun sequence.
ACCESSION   DAOPKU010000663 DAOPKU010000000
VERSION     DAOPKU010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92053_length_1597_cov_5.034371"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..413
                     /locus_tag="PK849_14750"
     CDS             111..413
                     /locus_tag="PK849_14750"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPE67439.1"
                     /translation="MSSRRNFSAEFKAKVVLEMLREEETLSQIAAKHKIHPNLLQRWK
                     KTATEGLPGLFSDPRRKSAEDLGKDSTINDLYRQVGLLSMQLEWIKKKCGVDSFSS"
     gene            391..1257
                     /locus_tag="PK849_14755"
     CDS             391..1257
                     /locus_tag="PK849_14755"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPE67440.1"
                     /translation="MSTLSLHERRALVDWEEKEIPLSWQAWLLGLNRSGLYYVPAPLS
                     EEELLLRRRIDEINTLHPCYGSRRMRAVLSRGGLSVSRKRIQRLMRDMGILSIYPAPK
                     LSAGDESPKFPYLLRGVDIDHPNHTWGTDITYVRLRGGFMYLTVFLDWYSRYVVSWAF
                     SDSLSAGFVIEALEDAFKTARPKIINSDQGSQYTSKSYVERIRDESDGIAISMDGRGR
                     CMDNIFTERFWRSYKQEEVYLKEYESPREARRSTAEYIRHYNFNRPHQSLNYRTPWEV
                     YSGEKAEKVNIK"
BASE COUNT          408 a          386 c          432 g          371 t
ORIGIN      
        1 gcttaccatc gtcgggttcg gtgcgatggg aaaaactccc cgaaacatca ttccttataa
       61 tggaccccga aaatcggaca aaaagtcggg gggaggttaa ggtgtaaaag atgagttcgc
      121 gtcggaactt ttctgctgaa ttcaaggcaa aagttgtgct cgagatgctt cgtgaagaag
      181 aaactctcag ccagatcgca gccaagcaca aaatacatcc caacctgctt cagagatgga
      241 aaaagacggc cacggaaggg cttccgggat tgttctccga tccccgcagg aaaagcgccg
      301 aggatctcgg gaaggattct acgataaacg acctttacag acaagtcggg ttgctttcca
      361 tgcagctcga atggatcaaa aaaaaatgcg gtgtcgactc tttctcttca tgaacgcaga
      421 gcgctcgtcg actgggagga aaaagagatc cctctctcct ggcaggcctg gctgctcggt
      481 ctgaacagaa gcgggctgta ctacgtcccc gcgcctctct cggaggaaga actgctgctc
      541 cgcaggagga tcgacgagat caacacgttg catccgtgct acggctccag gaggatgcgg
      601 gcggtgctgt cgcggggggg attgtccgta tcgcgcaaac ggattcagcg tctcatgaga
      661 gacatgggaa tcctctcgat ataccccgct cccaaactga gcgcgggcga cgagagtccg
      721 aagttcccct atcttctgcg cggagtggac atcgatcatc cgaaccacac gtgggggacg
      781 gacatcacct acgtccggct gaggggcggc ttcatgtatc ttacggtgtt tctggactgg
      841 tactcccgct acgtggtctc gtgggcattt tcggactcgc tgtcggcggg gttcgtgatc
      901 gaggcgctgg aggacgcctt caagacggcc cgcccgaaaa tcatcaacag cgatcagggg
      961 tcgcagtaca cgagcaaatc gtatgtcgag cggattcggg acgaaagcga cggcatcgcc
     1021 atcagcatgg acggaagggg gcgttgcatg gacaacatct tcaccgagag attctggagg
     1081 agctacaagc aggaggaagt gtatctgaag gagtacgaga gtccccgcga ggctcgccgc
     1141 tcgacggcgg agtacatacg gcattataat ttcaaccggc ctcatcaatc gttgaattac
     1201 cggacacctt gggaggtgta ttcgggggaa aaggccgaga aggttaacat taagtagtcg
     1261 ctattctttt tcacacaaac taaaaattca atgatttttt gtcttgacaa ccgggtccac
     1321 tttaatgctc ccacgctttt taccataaac ggatgcggta caacactttt tggctctact
     1381 gatattaacc cacttacagg ccaatacgtc gcaacatatt attttatatt gtttgcgata
     1441 ccaatttttc cgataggaag atacctatta agccaaaatt gcaaaaaata tcgtttcttc
     1501 ggaaagttgc cgttgcggtt atttgataag ttgcacatag cgatttcaat aggtttaatc
     1561 tctctgctgt ttttaaaaat gtggtgaaag ggagttt
//
LOCUS       DAOPKU010000664         1595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_92284_length_1595_cov_2.262338, whole genome shotgun sequence.
ACCESSION   DAOPKU010000664 DAOPKU010000000
VERSION     DAOPKU010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92284_length_1595_cov_2.262338"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1595)
                     /locus_tag="PK849_14760"
     CDS             complement(<1..>1595)
                     /locus_tag="PK849_14760"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPE67441.1"
                     /translation="IAVDFPYELDSDYQKLYDDVLLFASDLMAAKRGSENQKRFRYWS
                     ALALLRGIMSSPRAGACMLENRAAGGPLPEEDDGSADGISPEHPHYERLEASDDELPT
                     PVLNRTELSVQQRARFSEFAERLRLMEEGSRDVKSASCVLQIRKWLDEGFTPIVFCRY
                     ILTAKYLAERLTETFGRNAVVECVTSEDPDEVRRARVNAMTPEAAGGKRRILVATDCL
                     SEGINLQHLFDAVLHYDLPWNPNRLEQREGRVDRFGQTRPEIRTCLLYGKDNPMDRVV
                     LRVLYNKAKIIRNNIGVTIPFPEDSKDFLDTIFRAILAEAEKKRKPSGQMELFDLEER
                     VRCEAELDRLVTVAEKKESALRTIFAQQSIKAQDLEEDLALVDAAVGRPETVWRFVQS
                     ALGELFGVRAAGDPHLLTIDVARSDFPDKLRNRLEEVSGKRNARLAFRAPVPEGRTYW
                     GRNHDAVEALCRTVLAESLNPPRDGASPPRVARAAVVLSGAVEERTVLYVLRARHVID
                     DLRAHKSMVAEEILLRGLAGAER"
BASE COUNT          243 a          478 c          565 g          309 t
ORIGIN      
        1 tccgttccgc tccggcgagg ccccgaagga gaatctcctc tgccaccatg ctcttatgcg
       61 cccgcaggtc gtcgatgaca tggcgggcgc ggaggacgta gagcacggtg cgttcctcca
      121 ccgcgccgga gaggacgacg gcggcgcggg cgacgcgggg cggggaggcg ccgtcgcggg
      181 gtgggttgag cgattccgcg aggaccgtgc ggcagagcgc ctcgaccgcg tcgtgattgc
      241 gcccccagta cgtccgcccc tcggggacgg gcgcgcggaa ggcgagacgg gcgttccttt
      301 ttcccgacac ctcttcgagc cggttccgca gcttgtcggg gaagtcggag cgcgcaacat
      361 cgatggtgag aagatgcggg tccccggcgg cacggactcc gaaaagctct cccagagcgg
      421 actgcacgaa gcgccatacc gtctccggcc gtccgacggc ggcgtcgacg agggcgagat
      481 cctcctccag atcctgcgcc ttgatgctct gctgcgcgaa gatcgtccgg agcgcggact
      541 cctttttttc ggcgacggtg acgagccggt caagctccgc ttcgcatctg acgcgctctt
      601 cgaggtcgaa cagctccatc tgtccggaag gtttgcgctt tttttccgct tcggcgagga
      661 tcgcccggaa gatggtgtcg aggaaatcct tcgagtcctc ggggaagggg atggtgacgc
      721 cgatgttgtt ccggatgatt ttggccttgt tgtagagaac cctgaggacg acgcggtcca
      781 tcgggttgtc cttaccgtag agaaggcagg tgcggatctc gggccgggtc tgtccgaagc
      841 ggtcgacgcg cccctcgcgc tgttcgaggc ggttggggtt ccacggaagg tcgtagtgga
      901 gcacggcgtc gaagaggtgc tggaggttga tcccttccga gaggcagtct gtcgccacca
      961 ggatgcgccg ctttcctccg gcggcttcgg gggtcatggc gtttaccctc gcgcggcgca
     1021 cttcgtcggg gtcctcgctg gtgacgcact cgacgacggc attccgtccg aaggtttcgg
     1081 tcagtcgttc ggcgaggtat ttcgccgtca ggatgtagcg gcagaagacg atgggcgtga
     1141 agccttcgtc gagccatttg cgaatctgaa gaacgcacga ggcgcttttg acgtcgcggg
     1201 agccctcctc catcaggcga agccgttcgg cgaactccga aaaccgcgcc ctctgctgga
     1261 cgctcagttc ggtgcggttc aggacggggg tgggcagttc gtcgtcgctc gcttcgaggc
     1321 gttcgtagtg ggggtgttcc ggagaaatac cgtcggcgct tccgtcgtcc tcctcgggga
     1381 ggggacctcc ggccgcgcgg ttctccagca tgcacgcgcc cgccctcggg ctggacatga
     1441 tgccgcgcag aagggccagc gccgaccagt agcggaatcg cttctggttt tcgctgccgc
     1501 gttttgcggc catgaggtcg gaggcgaaga gaaggacgtc gtcgtacagc ttctggtagt
     1561 ccgaatccag ttcgtagggg aagtcgacgg cgatc
//
LOCUS       DAOPKU010000665         1591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_92573_length_1591_cov_2.705078, whole genome shotgun sequence.
ACCESSION   DAOPKU010000665 DAOPKU010000000
VERSION     DAOPKU010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92573_length_1591_cov_2.705078"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1591
                     /locus_tag="PK849_14765"
     CDS             15..>1591
                     /locus_tag="PK849_14765"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPE67442.1"
                     /translation="MPNPEVVWRTLLVSAKASLALGAVPNAVRFLDQAIEVMESVSPS
                     DGTNSQATADKLDVYRTAVETAYSLYAASPSDENAAMLWRYLEGMKSRQWREMLATTG
                     AAFLDRLPESEEEHYQVLSMSAAQLLAKIAFLDFRGLAKEADEARAELQDVRGALGEI
                     TARYTVDSVRRVPTLEEVSDALHPDWAAADYYLSRELSFAFVLEKGKRPRLVQLPLDY
                     DSFFAYTLWMRTLPDAVEFQSTRANEAVMAAGVSSPRLAKQLFEPVAQLIGEKKKLLV
                     IPHDLLYTFPFETMSVASGDTWRFLLDEGWTFAELPSAFLLEKTRRPETAQGKKRLVV
                     MADPEYYPTLRKKHGAEEARKILLSGVENPLVQQIRDTFCKYMAPLRNARREGDYLVE
                     QWKGVGDVRSLMGADASEKMLYDQETKVWDAHHVHIVCHGYDRNSIPDLQPGLALSPV
                     ADSQNDSFAQMGELASLRWRSDLVVLSACDTGLGDLFIGDGMVGLNTVFLAGGAKGML
                     ISRWRVPDESAPGFMNV"
BASE COUNT          324 a          445 c          492 g          330 t
ORIGIN      
        1 ggagtactgg agggatgccg aatcccgagg tcgtctggag aacgctcctt gtctccgcga
       61 aggcctctct tgcattgggc gctgtaccga acgcagtccg ttttctggac caggcgatcg
      121 aggtcatgga gtccgtctcc ccctccgacg gtacgaattc acaagcgact gccgacaagc
      181 tggatgtgta ccgtacggcc gtagaaacgg cgtatagtct ctacgccgcg tccccttccg
      241 atgaaaacgc ggcgatgctg tggcgctatc tggagggaat gaagagcagg cagtggagag
      301 agatgcttgc gaccaccggc gccgcgtttc ttgaccgtct tcctgaaagc gaggaagaac
      361 actatcaggt actgagcatg tctgcggcgc agctgctggc gaagatcgcc tttcttgatt
      421 ttcgagggct tgcaaaggag gctgacgagg ctcgcgcgga acttcaggat gttcggggag
      481 cgctcggaga gataacggcc cggtacacgg tcgactccgt ccggcgagtt ccgacgcttg
      541 aggaagtttc cgacgcgctc catcccgact gggcggcggc agattactat ctgtcacgcg
      601 agctctcctt cgcatttgtg ctggaaaaag ggaagcgccc tcgccttgtg cagctccccc
      661 tggactacga tagttttttt gcgtacacgt tgtggatgcg tacgcttccc gacgcggtag
      721 aattccagtc gacacgggcc aacgaggctg tcatggctgc gggcgtttca tccccaaggc
      781 tcgcgaagca gcttttcgag cccgtggcgc agctcatcgg cgagaagaaa aaactcctcg
      841 tcatccccca tgatcttctg tacacctttc cctttgaaac catgtcggtt gcgagcggcg
      901 atacatggag gttcctgctg gacgaagggt ggacctttgc agaactcccg agcgcgtttc
      961 ttcttgagaa gacaagaaga ccggaaacgg ctcaggggaa gaagaggctt gtcgtgatgg
     1021 cagacccgga gtactatccg acgcttcgaa agaaacacgg agcggaagaa gcgcgaaaaa
     1081 tccttctcag cggcgtcgag aaccccttag tgcagcagat acgggatacg ttctgcaaat
     1141 acatggcccc gctccggaac gcgcggcggg aaggcgacta ccttgtcgaa caatggaagg
     1201 gcgtcggaga cgtacgttcc ctgatgggcg cggacgcctc ggagaagatg ctctacgatc
     1261 aggagacgaa agtgtgggac gcgcaccatg tgcatatcgt gtgccacggc tacgaccgga
     1321 acagcatccc ggatcttcag cccgggcttg ctctctcccc ggttgcggac agccaaaacg
     1381 attcgtttgc gcagatgggt gaactcgcct cgctgaggtg gcgttccgat ctcgtcgtcc
     1441 tttccgcctg cgacaccggc ctgggcgatc tcttcatcgg cgacgggatg gtcggtctga
     1501 acactgtctt tcttgccggc ggagcgaagg gaatgctcat ctccagatgg cgggtgccgg
     1561 atgagagcgc tcccggtttc atgaatgttt t
//
LOCUS       DAOPKU010000666         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_93364_length_1581_cov_3.615990, whole genome shotgun sequence.
ACCESSION   DAOPKU010000666 DAOPKU010000000
VERSION     DAOPKU010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93364_length_1581_cov_3.615990"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PK849_14770"
     CDS             complement(<1..839)
                     /locus_tag="PK849_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE67443.1"
                     /translation="MKLRNVLLAALLVFVVAGTAMAADTIKIGVYLPLTGRTAFGGQL
                     ELEGIQMAHKEMSEVLGKKVELVVVDNKTDKVEAANAVKRLIEYDKVVAVIGTYGSSL
                     AMAGGEVAEAAKVPMMGTSCTNPLVTQGKMYVFRACFIDPFQGAGAATYAINSLKMNK
                     AALLIDVANDYCVGLANFFKQSYKKLGGEIVSELKYNSGDQDFTAQLTEIISQNPDLL
                     FIPADFAEGAIIMKQARELGAEFQIMGGDAMDNPEIVNIGGEAVEGFIHTTFPYDPSM
                     TDM"
     gene            complement(930..>1581)
                     /gene="nrdD"
                     /locus_tag="PK849_14775"
     CDS             complement(930..>1581)
                     /gene="nrdD"
                     /locus_tag="PK849_14775"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPE67444.1"
                     /translation="SEEAFQLALAVTQYMNLKCQALSERYGLDIVLEQTPAESTAYRF
                     AKLDLKSCPETLDVVKGNPVTGEVYYTNSTHFPYNILMDPVRKIVEEGRFHPLISAGA
                     ITHIWMGEHKPDARALSSFIRKVFRHSENAQVTFSPEFTVCNGCGVTSRGLRESCPSC
                     GSMDVDGITRITGYFTRTSSWNDGKRAELQDRVRTSIPDVPSEVGLSRQPSLALEK"
BASE COUNT          356 a          441 c          408 g          376 t
ORIGIN      
        1 ctcatatcag tcatcgacgg gtcatagggg aaggtcgtgt ggatgaagcc ttcaacagct
       61 tctccgccga tattgactat ttcggggttg tccatggcgt cgccgcccat gatctggaac
      121 tcggcgccca gctctctggc ctgcttcata ataatggcgc cctcggcaaa gtcggccggg
      181 atgaagagaa ggtcaggatt ctggctgatg atctccgtaa gctgcgcggt aaagtcctga
      241 tcgccggagt tgtacttcag ctccgatacg atctcgccgc caagcttctt gtaggactgc
      301 ttgaagaaat tggcaagtcc cacgcagtag tcgttcgcca cgtcgatgag aagcgcggcc
      361 ttgttcattt tgagagagtt gattgcatac gtcgcagcgc ccgctccctg gaagggatcg
      421 atgaagcatg ctctgaagac atacatcttg ccctgggtaa ccagcgggtt ggtgcaggag
      481 gtccccatca tggggacctt cgcggcttcg gcgacctcgc cgcccgccat ggcgagagag
      541 gatccgtacg ttccgatgac ggcaacgacc ttatcatatt cgataaggcg cttcacggcg
      601 ttggcggcct cgaccttatc cgtcttgttg tcgacgacga caagttcgac tttctttcca
      661 agcacctccg acatttcctt gtgcgccatc tgaatgcctt caagttccaa ctgtccgccg
      721 aaagcggttc ttccggtcaa gggaagatat acgccgatct tgatcgtatc ggcggccata
      781 gccgttcccg caacaacgaa aaccaacagt gcagcaagca gtacatttcg aagcttcatt
      841 cggttccctc ctcagatatt gatattcgac ttcacgataa attatacaca aaaactggga
      901 cttccggaag ccctttcttt tctttatttt tacttttcaa gggcgaggga aggttgtcgc
      961 gaaagaccga cttcactggg tacatcggga atcgaggtac gaaccctgtc ctgaagctca
     1021 gccctctttc cgtcgttcca gctcgaagtc ctggtaaaat acccggtaat tcgcgtaata
     1081 ccgtccacgt ccatacttcc gcaggaagga caactctctc gaagaccgcg cgacgtcacg
     1141 ccgcagccgt tgcaaacagt gaattccggg ctgaaagtaa cttgcgcgtt ttcggagtga
     1201 cgaaaaactt tccgtataaa cgaagaaaga gcgcgggcat ccggtttatg ctcgcccatc
     1261 caaatgtgcg taatggcccc ggcggagata agcggatgaa aacgtccctc ctctacaatc
     1321 ttccgcacag gatccataag gatattgtac gggaagtgcg tgctgttggt gtagtacacc
     1381 tctcccgtga cggggttgcc cttcactacg tcgagcgtct ccggacacga cttaagatcc
     1441 agttttgcaa aacgatacgc tgtgctctcg gcgggagtct gctcaagcac gatatcgagt
     1501 ccatatcgtt ccgaaagagc ctggcacttc aaattcatgt actgcgtaac cgccagcgcg
     1561 agctggaacg cctcttccga t
//
LOCUS       DAOPKU010000667         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_93386_length_1581_cov_2.839450, whole genome shotgun sequence.
ACCESSION   DAOPKU010000667 DAOPKU010000000
VERSION     DAOPKU010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93386_length_1581_cov_2.839450"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1581)
                     /locus_tag="PK849_14780"
     CDS             complement(<1..>1581)
                     /locus_tag="PK849_14780"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE67445.1"
                     /translation="VDHHDDAYAWARNRKCSVHPETGSLQVFEVQETVREAEQSPAPE
                     AVPPLFEGVKEKYLLRMGVPSELLPAVREIRSEEELNRLAQSLPREAGDALSLLHLGF
                     SPEEILQDLELKKPDAKVDTEDFAKALQTDQSRSRFFVAEDDLELQAILQAPLEKWRV
                     FLHPTQRKIVEKDWNGPVRVLGGAGTGKTVAAVHRARWLVQSVLKDPNERVLFTTFTV
                     NLAEDIRSNLAKLCSDDVMKRIEVINLDRWVSQFLKARGYEMKLLFDDEKRRDFWGRA
                     MAYKPVDIDLPESFWIEEWEKVIQPQEITGEKEYFQADRKGRGASLDRKQRKAVWPVF
                     EEYRALLSENGYSEPDDAMRDARKLLESSGEKLPYRSIVVDEAQDLGPLAFKLIRRMV
                     PEGNNDIFIVGDAHQRIYKRRTVLSRCGIEVRGRSRKLRINYRTTEETKEWAVALLEG
                     RDFDDLDGGRDSQKGYRSLLRGQPPRLEGFPSFEDEAGFLAAYLNELQASGEPLRNIC
                     LVARTNSLLEQYRSALKAK"
BASE COUNT          292 a          514 c          414 g          361 t
ORIGIN      
        1 ctttgctttc aaggcggaac ggtactgctc aaggaggctg ttcgtcctcg cgacgaggca
       61 gatattccgg agcggttcgc cggaagcctg caactcattc aggtacgccg ccaggaaacc
      121 ggcctcgtct tcgaatgacg ggaagccctc caggcgcggc ggctgtcccc gcagcagcga
      181 acgatacccc ttctggctgt cccgccctcc gtcgagatcg tcgaaatccc ttccctcgag
      241 aagcgccaca gcccactcct ttgtttcttc cgtggtgcgg taatttatcc tgagcttcct
      301 gcttcgtccc cggacttcta ttccgcatcg ggaaagaacg gtccggcgct tgtatattct
      361 ctggtgggcg tcgccgacga tgaagatatc gttgtttccc tccgggacca tgcgccggat
      421 caatttgaag gcgagcggtc caagatcctg cgcctcgtcc acgacgatgc tcctgtaggg
      481 cagcttctcc cccgatgatt cgagcagctt ccgtgcatcc ctcatggcgt cgtccggttc
      541 gctgtacccg ttttcggaaa ggagagccct gtactcctcg aataccggcc acacggcctt
      601 tctctgtttc ctgtcgagcg aagcgccgcg ccccttccgg tcggcctgaa aatactcttt
      661 ttcgccggtg atctcctggg gttgaatcac cttttcccac tcctcgatcc aaaaactctc
      721 aggcagatcg atatcgacgg gtttgtaggc catcgctcgt ccccagaagt ctcttctctt
      781 ttcgtcgtcg aagagcagtt tcatttcata ccctctcgcc ttcaaaaact gagagaccca
      841 ccggtcgaga ttgatgactt cgattcgctt catcacatcg tcggaacaga gcttcgccag
      901 gttcgagcgg atatcctcgg caagattcac ggtgaacgtg gtaaaaagga cgcgttcgtt
      961 cggatccttc agaaccgatt ggacgagcca tcgggcccgg tggacggccg cgaccgtctt
     1021 gcccgttccc gcgcctccga gcacccgtac cggaccgttc cagtcctttt ccacgatctt
     1081 cctctgggta gggtggagaa agacccgcca cttttcaaga ggcgcctgca ggatcgcctg
     1141 aagctccaga tcatcctcgg cgacgaagaa cctgctccgg gactggtcag tctgaagcgc
     1201 cttcgcaaaa tcctccgtat ccaccttggc atccggcttc ttcagttcca gatcctgaag
     1261 tatctcttcc ggagaaaacc cgagatggag cagcgagagc gcgtcccccg cctccctggg
     1321 aagggactgc gccaggcggt tcagttcctc ttcggatcgt atctcccgta cggcgggcag
     1381 cagttccgaa ggaacgccca tcctgagcag atacttctcc ttcacgcctt cgaaaagagg
     1441 tggaaccgct tccggagcag gagattgttc cgcctcccgt accgtctcct gaacctcgaa
     1501 aacctgaagg ctccccgtct ccggatgcac ggagcacttt ctgttccgcg cccacgcata
     1561 ggcgtcgtca tggtggtcga c
//
LOCUS       DAOPKU010000668         1574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_93961_length_1574_cov_4.219223, whole genome shotgun sequence.
ACCESSION   DAOPKU010000668 DAOPKU010000000
VERSION     DAOPKU010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93961_length_1574_cov_4.219223"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK849_14785"
     CDS             <1..382
                     /locus_tag="PK849_14785"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67446.1"
                     /translation="VVDTEEFNRRDSRALLASLVSTHREAFALLKEGVERGDPAEVGE
                     AATLSAEACRRILEKPLLERCRALVRSLGGYGVCVAHSGTLYGLLLPVALLNEKEHVT
                     EAAARALPEAAEIRLHALAAGGPR"
     gene            409..1335
                     /gene="cbiB"
                     /locus_tag="PK849_14790"
     CDS             409..1335
                     /gene="cbiB"
                     /locus_tag="PK849_14790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HPE67447.1"
                     /translation="MEVFVVLFAAAVWDLLLGEPSSKVHPVVWMGKYIGAFWNRRPSG
                     ADDRKMLIFGGVLVVSGALLFSLPLRFLKLLPGLTALLLTIPLLKSVFSISALINAGE
                     SILRALELGDVPEARRLLSWSLVSRNTEDLSQEEVVGAAIESLAENLTDSLASPLFFY
                     AIFGLPGAFFYRFCNTCDSMIGYRGGDTEWGGKIAAKLDDVLNWIPARVTSILLLGAG
                     VLAKEDVIKGAEALRDERGNTASPNAGWTMSVMAGMLRVTLEKRGQYALAGGTEPLCT
                     QALRRCVKVVRIAAALILAFVAALGGLHALFG"
     gene            1319..>1574
                     /locus_tag="PK849_14795"
     CDS             1319..>1574
                     /locus_tag="PK849_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67448.1"
                     /translation="MPFSVRKELLLAAETVHGGLDYAKLRSEGVEPESILDFSVSTNP
                     LPPHPEVERAVARAAFDRYPDSSSGALRRAIAEANGLSSDE"
BASE COUNT          239 a          476 c          563 g          296 t
ORIGIN      
        1 cgtcgtggac accgaggagt tcaacaggag ggacagccgc gctcttctcg cctcgctcgt
       61 ttccacgcac agggaggcgt tcgccctgtt gaaggagggc gtggagcggg gagaccccgc
      121 cgaagtgggg gaggctgcaa cgctcagcgc ggaggcgtgc cgccgcattc tggaaaaacc
      181 gctgttggag cggtgtcgcg cgctcgttcg ttcgctcggg gggtacggcg tctgcgttgc
      241 gcacagcggc acgctctacg gtctgctcct ccccgtcgcc ctgctgaatg aaaaagagca
      301 tgtaacggag gccgccgcgc gcgcgctgcc ggaggcggcg gagattcgtc ttcacgccct
      361 ggcggccggg ggaccgcggt agaatccgtt cgaaaggaag gtattcgcat ggaagtgttt
      421 gtcgttctct tcgccgccgc agtgtgggat ctcctgcttg gggagccctc gtcgaaggtg
      481 catcctgtgg tctggatggg aaagtacatc ggcgcgttct ggaaccgccg tccgtctggt
      541 gcggacgacc ggaagatgct catcttcggc ggcgtgctcg tggtgtcggg ggcgctgctc
      601 ttctcccttc cgctgcgttt tttgaagctc cttccggggt tgacggcgct gctgctcacc
      661 attccgctgc tgaagagcgt cttttccatc tccgcgctga tcaacgcagg agagagcatc
      721 ctgcgggcgc ttgaactcgg cgatgtgccg gaggcccggc ggctgctttc gtggagcctg
      781 gtcagccgga acacggagga tctgtcgcag gaggaggtcg tcggcgcggc gatagagtcg
      841 ctggccgaaa acctcaccga cagcctggcg tcgccgctct tcttctatgc gatcttcggc
      901 cttccggggg ccttctttta ccgattctgc aacacctgcg actccatgat cggctaccgg
      961 ggaggggata cggagtgggg agggaagatc gccgcgaagc tcgacgacgt cctgaactgg
     1021 attccggctc gcgtgacctc gattctgctc cttggggcgg gcgtgctcgc caaggaggac
     1081 gtcatcaaag gcgccgaggc gctgcgcgat gaacggggga acaccgccag ccccaacgcg
     1141 gggtggacca tgtcggtgat ggcggggatg ctcagggtga cgctggagaa gcgcggccag
     1201 tacgcgcttg ccggagggac cgagccgctg tgcacgcagg cgctgcgccg ctgcgtaaag
     1261 gtggttcgca tcgccgccgc gttgattttg gccttcgtcg cggcgctggg agggctccat
     1321 gcccttttcg gttagaaagg aacttctgct cgccgcggag acggtccacg gcgggctcga
     1381 ctacgcgaaa ctgcggagcg aaggggtgga gccggagagc attctggact tcagcgtctc
     1441 gacgaacccg ttgcccccgc accccgaggt ggaacgggcc gtcgcgcggg cggcgttcga
     1501 ccggtaccct gactcctcgt cgggggcgct ccggcgcgcc atagccgagg cgaacggtct
     1561 ctcgtccgac gaag
//
LOCUS       DAOPKU010000669         1573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_94060_length_1573_cov_3.855072, whole genome shotgun sequence.
ACCESSION   DAOPKU010000669 DAOPKU010000000
VERSION     DAOPKU010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94060_length_1573_cov_3.855072"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PK849_14800"
     CDS             <1..474
                     /locus_tag="PK849_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPE67449.1"
                     /translation="VRAHELHTGEEIWTQTEGKLDAFVDFAGTGGSFTGCALALKKHN
                     PRIRCYLLEPETAAFLGKGEVVNANHKIQGGGYSRTLPLLAPSLVEDYLAASNDDAIE
                     GAHALAVYEGIFGGFSSGANLFAAVSLLRGREKGNAVAFLVCDSGLKYLSTDLYL"
     gene            627..>1573
                     /gene="alr"
                     /locus_tag="PK849_14805"
     CDS             627..>1573
                     /gene="alr"
                     /locus_tag="PK849_14805"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPE67450.1"
                     /translation="MTLRPTRMEVRLDNIRANYGAIRKHLDEHGGAAAKIFGVIKANA
                     YNLGAVPVAWALREAGADYFIVATPDEALELRAGGIVDPVLVLGASPYEAAPEYVRYG
                     IRATITDMEMAHALSAAAEKSGKKAFVHIKIDTGMGRIGFRPDEATETVARIAALPGV
                     VCEGAFTHFATADDADLAYVHEQHALFLKALDLIAAKGISIPLRHCCNSGGTLSFPQW
                     AMDGVRPGQIVCGMYPTEHVPRTVLVLPGFSFKTAVAALRTLPPGQGVSYGLTYVTRS
                     EERLAVLPLGYADGFARPLSNRGEVLIRGTRCRVTGRIC"
BASE COUNT          280 a          478 c          550 g          265 t
ORIGIN      
        1 gtgcgggcgc acgagctgca caccggggag gagatctgga cgcagacgga ggggaagctg
       61 gacgccttcg tcgacttcgc cggaacgggg ggctccttca cggggtgcgc gctggcgctg
      121 aagaagcata atccgcgcat ccgctgctat ctgctggaac cggagaccgc cgcgtttctt
      181 ggcaaggggg aggtcgtcaa cgcgaaccac aagattcagg ggggcgggta cagccggacg
      241 ctcccgctgc tcgccccgtc gcttgtggag gactacctcg ccgcaagcaa cgacgacgcg
      301 atcgaagggg cgcacgcgct tgccgtgtac gagggcatct tcggcggttt ttcgtcgggg
      361 gcgaacctct tcgcagccgt ctcgcttctg cgggggcgcg aaaaggggaa tgcggtggcc
      421 tttctggttt gcgactccgg gctgaaatat ctgagcacgg atctctacct gtaggcgggg
      481 gggagcgcct tccaagagag aggacgcgcc tattcaacat agttttatct tttgcatcga
      541 tgccgaaaat aaatacattt cgcgccgtcc tttgacatat aatagacgca cgcgcgcaga
      601 gcgcatcttt tggggaggtg gcatcgatga cgttgcgtcc tacgcggatg gaagtccggc
      661 tggacaacat acgggccaac tacggggcaa tccggaagca ccttgacgaa cacggcggcg
      721 cggccgcaaa aatcttcggc gtgatcaagg cgaacgcgta caacctgggg gcggtccccg
      781 tggcgtgggc gctccgcgag gcgggagcgg actacttcat cgtggccacg ccggacgaag
      841 cgctcgaact gcgcgcaggg gggatcgtcg atcccgtgct ggtgctgggg gcgtcgccgt
      901 acgaagcggc ccccgaatac gtgcggtacg gcatacgggc gaccatcacc gacatggaga
      961 tggcgcacgc gctctccgcg gcggcggaaa aatcagggaa aaaggcattt gtccacatca
     1021 agatcgacac cggaatgggg cgcatcggct tccgtccgga cgaagcaacg gagacggtcg
     1081 cgcggatcgc cgcgctgccg ggagtggtct gcgaaggggc gtttacgcac ttcgccacgg
     1141 cggacgacgc cgatcttgcc tacgtgcacg agcagcacgc gctcttcctg aaggcgctcg
     1201 acctgatcgc ggcgaaggga atctccattc cgctgcgcca ctgctgcaac tcgggaggga
     1261 cgctctcctt cccgcagtgg gcgatggacg gcgtccggcc ggggcagatc gtctgcggga
     1321 tgtaccccac cgagcacgtc ccccgcacgg ttctcgttct tccgggcttc tcgttcaaga
     1381 cggccgtggc ggccctcagg acgctgccgc cggggcaggg ggtcagctac gggctgacgt
     1441 acgtcactcg ctccgaggag cggctcgccg tcctcccgct ggggtacgcc gacggtttcg
     1501 cccgcccgct ctcgaacagg ggagaggtgc tgatccgggg gacgaggtgc cgcgtgacgg
     1561 ggcgcatctg cat
//
LOCUS       DAOPKU010000670         1564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_94844_length_1564_cov_5.352551, whole genome shotgun sequence.
ACCESSION   DAOPKU010000670 DAOPKU010000000
VERSION     DAOPKU010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94844_length_1564_cov_5.352551"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PK849_14810"
     CDS             <1..663
                     /locus_tag="PK849_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HPE67451.1"
                     /translation="MRTEGGAGKTEMWYVLDREPGAFLYCGFERDVSREELTRRIADG
                     TVTEVLHKAPVEPGDVFFMEAGTVHAIGAGILIAEIQQSSNTTYRVFDYGRRGPDGKE
                     RPLHIEKALDVARRGPACSVPPGSRPPVILPGSTLRRLARCAHFSVELLELSEHCEYR
                     TDETSFLSLLCLEGSARLAEGEWGFDIAKGDSVFVPARKGSVFLEGRGTFLLTTVPDG
                     EL"
     gene            801..>1564
                     /gene="guaA"
                     /locus_tag="PK849_14815"
     CDS             801..>1564
                     /gene="guaA"
                     /locus_tag="PK849_14815"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPE67452.1"
                     /translation="MNNIVILDCGSQFTQLIARRIRELKVHSEILPWDAPIERIKSAA
                     LSGVVISGGPMSVSDEGAPTVPEELFQLGVPVLGVCYGMQLATKLFGGVVTSGSSREY
                     GRTAISVKEESALFSGTAASFEVWMSHGDHIDRLAPGFRCVAETANGVPAAMENPEKG
                     VWGLQFHPEVVHTAFGTEILSNFLFSVCGCVPDWDLGNWIDTMTEEIRRKVGPDRVIC
                     GLSGGVDSSVAAVLTARAIGDRLECIFVNNGLLRLN"
BASE COUNT          279 a          461 c          528 g          296 t
ORIGIN      
        1 atgcgcacgg aggggggcgc cgggaagacg gagatgtggt acgtactcga ccgggaaccc
       61 ggggcgtttc tctactgcgg ctttgaacgg gatgtttcca gggaggagct gacgcggcgg
      121 atagcggacg gcacggtgac ggaggtgctg cacaaagcgc ccgtggagcc gggagacgtg
      181 ttcttcatgg aggccggaac ggtccacgcc atcggcgccg ggatactgat cgccgaaatc
      241 cagcagagct cgaacacgac gtacagggtc ttcgactacg gccgccgcgg tcccgacgga
      301 aaggagcggc cgctgcacat cgaaaaggcg ctcgacgtcg cccgcagggg acccgcatgc
      361 tccgtccctc caggtagccg gcctcccgtc atcttgcccg gaagcacgct ccgccgcctc
      421 gcgagatgcg cgcacttctc cgtggagctt cttgagcttt cggaacactg cgagtaccgg
      481 acggacgaaa catccttcct ctctctcctc tgtctcgaag gctcggcgag gttggccgag
      541 ggggagtggg gattcgatat cgcgaaggga gacagcgtct tcgtccctgc gcggaaagga
      601 tccgtcttcc tggaaggacg cggaactttt ctgctgacga ctgttccgga cggagagctc
      661 tgagagggaa cggggcggtt ttcgcggcgc tttccgtttc ctcttgtgga acggcgaggg
      721 gctttctggt aagatacaac ggggtgtgaa agccgaactt tccggccccg gtttttctat
      781 atttcagggg gaatggactt gtgaacaaca tcgtcatact cgattgcggc tcccagttca
      841 cgcagctcat cgcgagaaga atcagggagc tgaaggtaca cagcgagatt ctgccgtggg
      901 acgctcccat cgagcgaatc aagagcgcgg ctctgtctgg cgtcgtcatc tccggcggac
      961 cgatgagcgt ctccgacgag ggcgccccga cagtaccgga ggagctgttt cagctcggcg
     1021 ttccggtcct cggcgtctgt tacgggatgc agctcgcgac gaagctcttc ggcggcgtcg
     1081 tcacgtcggg cagttcccgt gagtacggac gcacggcgat ctccgtcaag gaggagtcgg
     1141 cgcttttttc cggcacagcc gcttcgttcg aagtctggat gagccacggc gatcacatcg
     1201 accgtcttgc tcccggattc cgctgcgtcg ccgaaaccgc gaacggcgtt ccggcggcga
     1261 tggaaaaccc ggagaagggc gtctggggac tccagttcca ccccgaggtg gtgcatacgg
     1321 ccttcgggac ggagatcctt tcaaactttc tcttctcggt ctgcggatgc gtgcccgact
     1381 gggatctcgg caactggata gacaccatga cggaggaaat ccgccggaag gtcggaccgg
     1441 acagggtgat ctgcggcctc tccggcgggg tcgactcgtc cgtggcggcg gtgctcacgg
     1501 cgcgggccat cggcgaccgg ctggagtgca tcttcgtcaa caacgggctg ttgcgcctga
     1561 acga
//
LOCUS       DAOPKU010000671         1561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_95097_length_1561_cov_5.634130, whole genome shotgun sequence.
ACCESSION   DAOPKU010000671 DAOPKU010000000
VERSION     DAOPKU010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95097_length_1561_cov_5.634130"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PK849_14820"
     CDS             <1..488
                     /locus_tag="PK849_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPE67453.1"
                     /translation="REEAAEREGVRFFAADLEDWDSLRRHGLTGRSLRYLLHYDHRLA
                     RHFGVEGRPPLLSRSVVDLVSRFRLDFLYGGGLLKYPMRLLFSEIPESVRFEARKTGF
                     WHNGPSLPDLRGEVLRMLETTLLGNLVARPEAVGSMNPGALWRFLSAETLLEEDVRKG
                     A"
     gene            575..847
                     /locus_tag="PK849_14825"
     CDS             575..847
                     /locus_tag="PK849_14825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HPE67454.1"
                     /translation="MSMESAVAFYERLEKDVELQEKLKELGTKENISQYVKGELGYDF
                     TLEEMQKVIFERNPEMTDEELEAVVGGDQLLEIISQIFMSLLGAAA"
     gene            885..1157
                     /locus_tag="PK849_14830"
     CDS             885..1157
                     /locus_tag="PK849_14830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HPE67455.1"
                     /translation="MSIESAVAFYERVEKDTALQAKLKELNTKENISQYVKGELGYDF
                     TLEEMQKVIFERNPEMTDEELEAVVGGDQLLEIISQIFMSLLGAAA"
     gene            1195..1491
                     /locus_tag="PK849_14835"
     CDS             1195..1491
                     /locus_tag="PK849_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HPE67456.1"
                     /translation="MSIESAVAFYERVEKDTALQAKLKELNTKENISQYVKGELGYDF
                     TLEEMQKVIFERNPEITDEELEAVVGGSITLIGIFSLGMAISGLVITFASMAAA"
BASE COUNT          330 a          368 c          530 g          333 t
ORIGIN      
        1 cgcgggagga ggcggccgaa cgggagggag tgcgcttctt cgccgccgat ctggaggact
       61 gggactcgct gcgccgtcac ggtttgacgg ggagaagcct gcgctacctt ctgcactacg
      121 accaccggct tgcgcgccat ttcggggtcg aggggcgccc gcctcttctg agccggagcg
      181 ttgtcgatct ggtctcgcgc ttccggctgg attttctcta cggcgggggg ttgcttaaat
      241 acccgatgcg cctgctcttt tcggagattc cggagagcgt gcgcttcgag gcgcgcaaga
      301 ctggcttctg gcataacggc ccttcccttc cggatctccg cggcgaagtg ctgcgtatgc
      361 tggagacgac gcttctcggg aatctcgtcg cccgtccgga ggctgtgggg agtatgaatc
      421 cgggcgctct ctggcgcttt ctctcggcgg agacattgct ggaggaggat gtgcggaagg
      481 gggcttgact ctgacacggt gtgaaggtgt agtgtagcgt tggatcggaa cttttccggc
      541 agatgaagag aaaatcaaca gtgaggtgtt gcgaatgtcg atggaatccg cagttgcgtt
      601 ctacgagcga ttggagaagg atgtcgagtt gcaggagaag ctgaaggagc ttggaacgaa
      661 ggagaacatc tcgcagtacg tgaaggggga actcgggtac gacttcacgc tggaggagat
      721 gcagaaggtg atcttcgagc gcaaccccga gatgaccgac gaggaattgg aggcagtcgt
      781 cggtggagat cagcttcttg aaatcatctc ccagatattt atgagccttc ttggagcggc
      841 cgcctgaagc ggcgataatt cttgaacagc gggaggtacg gaaaatgtcg atcgaatccg
      901 cagttgcgtt ctacgaacgg gttgagaagg ataccgcgtt gcaggcgaag ttgaaggagt
      961 tgaacacgaa ggagaatatt tcgcagtacg tgaaggggga actcgggtac gacttcacgc
     1021 tggaggagat gcagaaggtg atcttcgagc gcaaccccga gatgaccgac gaggaattgg
     1081 aggcagtcgt cggtggagat cagcttcttg aaatcatctc ccagatattt atgagccttc
     1141 ttggagcggc cgcctgaagc ggcgataatt cttgaacagc gggaggtacg gaaaatgtcg
     1201 atcgaatccg cagttgcgtt ctacgaacgg gttgagaagg ataccgcgtt gcaggcgaag
     1261 ttgaaggagt tgaacacgaa ggagaatatt tcgcagtacg tgaaggggga actcggctac
     1321 gacttcacgc tcgaggagat gcagaaggtg atcttcgagc ggaaccccga gatcacggac
     1381 gaagagctgg aggccgtcgt ggggggatcc attacgctga tagggatttt ctccctcggg
     1441 atggccattt cggggcttgt gatcacattc gcctctatgg ccgccgcctg atcgccccga
     1501 cctgtttctc cgggagatcg gccgcatgcg tgccgacgat gtgcgtatcg ttccgtgaag
     1561 t
//
LOCUS       DAOPKU010000672         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_95696_length_1554_cov_4.060040, whole genome shotgun sequence.
ACCESSION   DAOPKU010000672 DAOPKU010000000
VERSION     DAOPKU010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95696_length_1554_cov_4.060040"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..>1554)
                     /gene="proS"
                     /locus_tag="PK849_14840"
     CDS             complement(219..>1554)
                     /gene="proS"
                     /locus_tag="PK849_14840"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPE67457.1"
                     /translation="VIRPTGYALWESIQRHFDDAFKETGHVNAYFPLLIPNSFLEKEA
                     EHVEGFAPECAVVTHAGGEELEEPLIVRPTSETVIGHMYSKWIQSWRDLPILINQWAN
                     VMRWEKRPRLFLRTSEFLWQEGHTAHATREEAMEETLKMLEVYRRIMTDILALPVIPG
                     EKTEGERFPGADNTYTCETMMSDKKALQAGTSHFLGQNFAKAFEIQFQNQDGNLEYAW
                     TTSWGASTRLIGALIMTHSDDDGLILPPRVAPVKVAILPISTDDEMIASTLLPKAAEI
                     SAQLDSRLGGRFTVVDKQFHLRPGDRFFSHLQRGVPLRIELGEKELASGTARVVRRDT
                     GAKVDIPLDALAERIPQLLEEIHTALYEKALAFRKENTRFVSGYEEFKSAIEEQGGFV
                     ETFFAGTSDDEKKIKEETGATARCMPLESSETGVCMLTGKTGARRTLFAKAY"
BASE COUNT          287 a          460 c          437 g          370 t
ORIGIN      
        1 cctcttcttc tcgctcttat aaaactgtca aggttctgtt cgggaacttc tgcttcaact
       61 tccgttttcg cttccgtccc cgctcttttc cgccgccgct cgggcggctc aaagagcgcg
      121 ccgtttcaaa gacgcgaggg gtataatacc acttttgcag gggttgtcaa ctcgtaattg
      181 cggctttttt attttccaaa tcaaaagtct tcttgaggtt agtacgcctt tgcgaataga
      241 gtccgcctgg cgccggtctt ccccgtcagc atgcacactc ctgtttcact cgactccagc
      301 ggcatgcagc gggccgtggc gccggtctcc tccttgatct tcttttcgtc gtcggaggtt
      361 cccgcaaaga atgtctcgac aaaaccgccc tgctcctcta tcgccgactt gaactcttcg
      421 taaccggaaa cgaaacgggt gttctccttc cggaaggcaa gggctttctc atagagggct
      481 gtatggattt cctcaagaag ctgcgggatt ctctcggcga gagcatcgag cggaatatcg
      541 accttcgcgc ccgtgtctct cctgacgacg cgcgccgttc cggaggcgag ctccttctct
      601 cccagctcga tacgaagagg cacgcccctc tgaaggtgcg aaaagaatct gtcgcccggg
      661 cgaaggtgga actgtttgtc caccactgtg aaccgccctc cgaggcgaga gtcgagctgc
      721 gcggagatct cggccgcctt gggaagaagg gtggacgcaa tcatctcgtc gtcagtgctg
      781 atgggaagga ttgcgacctt tacaggagca acgcgcggcg gcagaatgag gccgtcgtca
      841 tcggaatgcg tcatgatgag cgcgccgata agacgtgtag aggctcccca gctcgtcgtc
      901 caggcgtatt cgagattgcc gtcctggttt tggaactgaa tttcgaaggc cttggcgaaa
      961 ttttgcccga ggaagtggct tgttcccgcc tgaagcgcct ttttatcgct catcatggtc
     1021 tcgcaagtat aggtattgtc ggcgccgggg aagcgctccc cctccgtttt ttctccgggt
     1081 atcaccggca gcgcgagaat gtccgtcatg attctgcggt agacttcgag cattttcagc
     1141 gtctcttcca ttgcctcttc cctggtggcg tgggcggtat gcccctcctg ccagaggaac
     1201 tcggacgtac gaaggaagag ccggggacgc ttctcccagc gcatcacgtt cgcccactgg
     1261 ttgatgagga tgggaaggtc gcgccacgac tggatccact tgctgtacat gtgaccgatc
     1321 accgtttcgg acgtcggacg gacgatcagg ggctcctcaa gctcttcgcc ccccgcatgc
     1381 gtgaccacag cgcactccgg ggcgaaccct tccacatgct ccgcctcttt ctcaaggaat
     1441 gagttcggga tcaaaagggg gaagtaggcg ttcacgtgcc ccgtctcttt aaacgcgtcg
     1501 tcgaaatgac gctggatgga ttcccagagc gcgtaccccg tagggcgtat gacc
//
LOCUS       DAOPKU010000673         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_95786_length_1553_cov_4.433912, whole genome shotgun sequence.
ACCESSION   DAOPKU010000673 DAOPKU010000000
VERSION     DAOPKU010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95786_length_1553_cov_4.433912"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PK849_14845"
     CDS             <1..824
                     /locus_tag="PK849_14845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="choline/carnitine O-acyltransferase"
                     /protein_id="HPE67458.1"
                     /translation="NFEHSALDGSQHARLAAAILKAGERASAGEWKASPPLPLSFLLS
                     EELAHELKKAERSFERLRENTRLRVLEFTSFGREKIKRLGMSPDAFCQTAMQATIFDL
                     YGKFLNAYEAVMTRQFLLGRTEAMRPVTPEIAAFVHARTAEHLKAASSAHTGRIAECR
                     EGRGVDRHLFGLKKMMELFGQEIGVEETPALFSSPGYLALSRNLLSTSSGPTECFRIY
                     GFGPVDDEGFGVRYLMFPDKLVFTMTSRTVMEHRLLAFRERLSENLEKIAALLSE"
     gene            complement(917..>1553)
                     /locus_tag="PK849_14850"
     CDS             complement(917..>1553)
                     /locus_tag="PK849_14850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPE67459.1"
                     /translation="VALTDPEMTATMPKNLTAFTGMDALVHAIEAYTCRVATPFSDAP
                     AITAMELIGKYLRRAWKDGGDLEAREGMLLGSLLAGMAFSHSDVAAVHCLAEALGAIY
                     DAPHGACNSIALPAIMEFSMDACVERYARVATCLGLSFATPEEGAKMAVEEVKQMARD
                     LELPSISVFGVKEEDFEEIAAKSEANSSNPDNPKILSKADYVHILRKLTTA"
BASE COUNT          309 a          461 c          453 g          330 t
ORIGIN      
        1 tgaacttcga gcactccgcc ctggacggtt cccagcacgc acggcttgct gcggcaattc
       61 tcaaagcagg agagagggcg agcgcggggg aatggaaagc ttccccgccg ctcccgctct
      121 cctttcttct ttcggaagaa ctcgctcatg aactgaaaaa ggcagagcgc tcatttgaac
      181 gtctgcgtga aaacacaagg cttcgcgtgt tggaattcac atccttcggg cgcgagaaga
      241 tcaaacgtct cggcatgagt ccggacgctt tttgtcagac ggcgatgcag gcgacgatct
      301 tcgatctgta cgggaaattc ctcaacgcgt atgaagcggt tatgacaagg cagttccttc
      361 tgggccgcac cgaggccatg cgtcccgtca cgccggagat agcggctttt gtgcatgcac
      421 gaacggcgga acacctgaaa gcggcttcct cggcgcacac cgggcgaatt gccgaatgca
      481 gggagggacg cggcgtcgac cgccacctct tcgggcttaa aaagatgatg gaactcttcg
      541 gacaggagat cggggtggaa gaaacgcctg cccttttctc ttccccgggg tacctcgcgc
      601 tctcgagaaa tctgctctcc acgagctccg gtcctacgga gtgcttccgc atctacggtt
      661 tcggacctgt tgacgacgag gggttcggcg tcaggtacct gatgttcccc gataaactgg
      721 tctttacaat gacaagccgt acggtaatgg aacaccgtct gctcgccttc agggagaggc
      781 tgtcggagaa ccttgaaaag atagccgctc tcctctccga ataaagagga gcgaaggagg
      841 cggtcctccc gctcaccctt cgctcctctt tttctccatt tatttcacct ttttctcata
      901 cattccgcgg taactgctat gccgttgtca gcttcctgag tatatggacg taatcggctt
      961 tcgagagaat cttcgggttg tcggggtttg acgagttcgc ctccgacttc gccgcaatct
     1021 cctcgaagtc ctcttccttg acgccgaaga cggagatcga aggcagttca agatcgcgcg
     1081 ccatctgctt gacctcctcc acagccatct tcgcgccctc ctccggcgtc gcgaaggaga
     1141 gtccgaggca ggtggcgacg cgggcgtacc gttccacgca cgcatccatc gagaactcca
     1201 tgatcgcggg cagagcgatc gagttgcagg cgccgtgggg cgcgtcgtat atagccccga
     1261 gagcttcagc aaggcagtgg acggctgcca cgtcggagtg gctgaaggcc attcccgcaa
     1321 gaaggctccc caggagcatt ccctcacgcg cttccaaatc gccgccgtct ttccaagccc
     1381 tccgcagata tttgccgatg agttccatcg ccgtgatcgc cggggcgtcg gaaaaaggcg
     1441 tggcgacgcg gcaggtgtag gcctctattg cgtggacgag agcgtccatg ccggtaaagg
     1501 cggtcaggtt cttcggcatg gtggccgtca tctcagggtc ggtgagcgca acc
//
LOCUS       DAOPKU010000674         1546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_96502_length_1546_cov_2.598256, whole genome shotgun sequence.
ACCESSION   DAOPKU010000674 DAOPKU010000000
VERSION     DAOPKU010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96502_length_1546_cov_2.598256"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1363
                     /locus_tag="PK849_14855"
     CDS             122..1363
                     /locus_tag="PK849_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPE67460.1"
                     /translation="MSFFGFLALFLFCFVLSAFFSASEMAYSGINKIRVKRFVEEGRP
                     GAVKAQELLRDFNGTLSAILIGGNIVDIFMTALATAVLTTLFGPIGAVYSTLLMTSLI
                     ILFGEILPKAFVKDRSEEFALSAAGMLHLLVRLFRPFTNFTRKLAAVLRRWAPGPADN
                     LPGVTHDELLSIVDAMHEEGVLPGAEQELIGNAINFNELEVWEVQTPRVDLFAINIRE
                     PLENVRNLVVKNHYSRVPVYEGTVDNIVGFLSEKDFLAKCVQGEPFDLKSVLTRPLLI
                     AGSASLMDAFRILQASRSHMAVILDEYGGTSGIVTMEDFLEELVGDIYDEHDDIKENF
                     TQVEENVFLVNADVYLSDLFDKFLHRPSPESESSTFGGWLLERFTILPEVGATVQWED
                     LTFQIAKINGQRIQKVRIIRQ"
BASE COUNT          318 a          407 c          435 g          386 t
ORIGIN      
        1 aaagaatatg tcctatttcc tggccgatcc gctgtctttc tgtgatttgt gcgtgcggga
       61 caatgtgccg gaggcagtat gcggagtgta tgcacggggg aggaaatatg tcgtgaatca
      121 gatgtcgttt tttggttttc tcgccctctt tctcttctgt tttgttctct cggctttttt
      181 ttcggcttcg gagatggcat attccggcat caacaaaatc agggtgaagc gcttcgtcga
      241 ggaggggcgt ccgggcgcag tgaaggctca ggagctgctg cgcgatttca acgggacgct
      301 ttcggcaatt ctcatcggcg ggaatatcgt cgacattttt atgacggcgc tggctactgc
      361 cgtgctgaca acacttttcg gtcccatagg cgcggtgtat tcgactcttt taatgacgtc
      421 tcttatcatc ctcttcggcg aaatactgcc gaaagcgttc gtaaaagacc gctccgaaga
      481 gtttgcgctc tccgccgcgg ggatgctgca tcttctggtg cgccttttcc gcccctttac
      541 gaacttcacc cggaagcttg cggcagtctt acgacgctgg gcgcccggtc ctgccgacaa
      601 tcttcccggc gtgacccacg acgagctgct ctcgatcgtg gatgcgatgc acgaagaggg
      661 agtgctgccg ggggcggagc aggagctcat cgggaacgcc atcaacttca acgagttgga
      721 ggtgtgggag gtgcagacgc cgcgagtgga ccttttcgcc atcaacatca gggagcctct
      781 cgaaaacgtc cgcaatctgg tggtcaaaaa tcactattcc agagttccgg tctacgaggg
      841 cacggtggac aacatcgtcg gtttcctcag cgaaaaggat tttcttgcca agtgcgtaca
      901 gggcgaacct ttcgatctga agagcgtgct cacccggcct cttctcatcg ccggcagcgc
      961 cagtctgatg gacgctttcc gcatccttca ggcgtcgcga agccacatgg ccgtcattct
     1021 ggacgagtac ggaggaacct ccggcatcgt caccatggag gattttctgg aggagcttgt
     1081 cggggatatc tacgacgagc acgacgacat caaagagaac ttcacccagg tcgaggagaa
     1141 cgtctttctg gtcaatgccg acgtctattt gagcgacctg ttcgataaat ttctccatcg
     1201 tccgagtcct gaaagcgaat caagcacatt cggcggatgg cttcttgagc gtttcacaat
     1261 cctcccggag gtgggggcta ccgtccagtg ggaggatctg acgtttcaaa tcgcgaaaat
     1321 caacggacag agaattcaga aagtgcgcat tatcagacag taaaatacaa caaggaaaca
     1381 gaacaaaagc cctctccacg ccggaggggg cttttgttct gtttcccggg caagaggcgt
     1441 tctttgtccg tttttttatt tctgcggtgc aaggcgctca agagccttct gtgcgaaatc
     1501 gtctccctga tcggcggctt tccggtacca ttcgagggct tttttc
//
LOCUS       DAOPKU010000675         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_96733_length_1543_cov_4.368280, whole genome shotgun sequence.
ACCESSION   DAOPKU010000675 DAOPKU010000000
VERSION     DAOPKU010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96733_length_1543_cov_4.368280"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..>1543
                     /locus_tag="PK849_14860"
     CDS             449..>1543
                     /locus_tag="PK849_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPE67461.1"
                     /translation="MSEKKIPLIWQIGIGFILGIVAGAVLGEKVQIVEPVGSIFLTLL
                     KMLIVPLVFSSLVVGAASIGDPRDLGRIGIKTIAFYLVTTAVAITIGLLLGNFLQPGV
                     GLAIGEGAAFKAPEAPSIKNVIMGLFPANPLKAAVDGNMLQIIVFALFLGVSAVLAGE
                     KGKPFINFFDSLAETMYKLTGIVMKIAPYGVFALIAVTVSKYGMSVLAPFAKVILAVY
                     VGCALQVILVYSGLITVFVKKSPFWFLKGIREAGLTAFVTRSSSATLPITMRNTQENL
                     GVSEKVSSFVLPLGATINMDGTALYQGVCALFVAQAYGLTLGMGAQLSIILTATLASI
                     GTAGVPGAGLIMLTLVLTAVGLPLEGAALVA"
BASE COUNT          317 a          452 c          394 g          380 t
ORIGIN      
        1 caagcaccac tcccccgctc tgcgcggacg cggggccgca aaacgccagc aaaagcaaaa
       61 aaagcgttgc gatcatccgt tttttcaaca tcgcaatccc ctcccgattc aaaacagcga
      121 gcacctctag aacctacata tcctaacaga attcggcctg actggccata ctgcgttgat
      181 tgttcggggt aaatctgcac aacttatctt cgatagactg gaattacggc gaagaatagg
      241 atatgcttta gggaagctcc gattacaggg ggtttcccct tgattgggct ttattgcatc
      301 aagttcctgc gaggccagat cggctttatt caaaggttcc ttagctatga atgaaatcgc
      361 gcgaggaggt ggttttttcg gccattcgct gtttccgttg ttctcttcct gaagttgcac
      421 catccatttc ttgaaggagg cggtactgtt gtcggaaaag aaaatacctc ttatttggca
      481 gataggcatt ggtttcattc tcggaatcgt agcaggcgcg gtgcttggcg aaaaggtcca
      541 gatagtcgag ccggtaggaa gcatcttcct cacactgctc aagatgctca tcgttcccct
      601 tgtcttctcc agccttgtcg tcggggcggc ttcgataggc gatcctcgag atctcggcag
      661 aataggcatc aaaaccatcg cattctatct tgtcaccact gcggtcgcga tcaccatcgg
      721 gcttcttctc ggcaacttcc tccagcccgg agtcggcctt gcaataggag aaggcgcggc
      781 attcaaggcg cccgaagctc cgagcatcaa gaacgttatc atgggtctct tccccgcgaa
      841 tccgctgaag gccgctgttg acggcaacat gctgcagatc atcgtctttg ctctcttcct
      901 gggagtttcc gcagtcctcg ccggagaaaa gggcaaaccg ttcatcaatt tctttgattc
      961 actggccgaa acaatgtaca aactgaccgg catcgtcatg aagatcgccc cctacggcgt
     1021 cttcgccctc atcgctgtga ccgtttccaa gtacgggatg tcggttctcg ctccgttcgc
     1081 caaggtcatt cttgcggtct acgtcgggtg cgcgctccag gtcatccttg tgtactccgg
     1141 cctgatcacg gtattcgtca agaagtctcc cttctggttc ttgaagggga ttcgtgaagc
     1201 cggactgacg gccttcgtca ctcgctccag ctccgcgacg cttccgatca ccatgagaaa
     1261 cacccaggag aatctcggcg tctccgaaaa ggtttcttcc ttcgtcctcc cgctcggagc
     1321 gacgattaac atggacggaa ccgcactcta ccagggtgtc tgcgccctct tcgtcgcgca
     1381 ggcctacggg cttacgctcg gaatgggagc gcagttaagc atcatcctca ctgcgaccct
     1441 ggcttcgatc ggaacggcag gcgtccccgg agcggggctt atcatgctca cacttgtcct
     1501 tactgcggtc ggccttcccc ttgaaggcgc ggcgctcgta gcg
//
LOCUS       DAOPKU010000676         1537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_97341_length_1537_cov_2.696356, whole genome shotgun sequence.
ACCESSION   DAOPKU010000676 DAOPKU010000000
VERSION     DAOPKU010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97341_length_1537_cov_2.696356"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1439
                     /locus_tag="PK849_14865"
     CDS             99..1439
                     /locus_tag="PK849_14865"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE67462.1"
                     /translation="MNRRLSMMLLLFGLSLAVILLGRSVWASEKPWLTLEKALEMAER
                     NNPLISASGERIAQARARLDRASAASLPQLGVSLLYQEVQNEPRYPVVPAGYAKAGFE
                     STWKAALTLNWLLYSGGAVRLNTESKRIALFGVEAREKRTRQTVLWGVAAAWYDLRRA
                     EGKLTVAEDVFALTKEHLQHVEALFRNGMTAKNEVLRVQVSVSEAELNLIRAENAVAV
                     EWKALERAVGVPLCGAFDLPPREESPELFSMPQDAVSVGLAHRPEMEALDLAARSASL
                     ASQAANAQGGPQVALVGEVYAADDEFFPQKQNDWKVTLLASWSFFDGGASSARAREAR
                     AAAEEFLFRMEDMKRQIELEISVARLNCESSLQRISTGRTMVESAEEDYRMALRRYTA
                     QVGTNIDVLDARVALSNARNQLVESVYDSLKARAEMEYAMGVIGSDGRGEERTK"
BASE COUNT          300 a          401 c          534 g          302 t
ORIGIN      
        1 ccacgggttg attatgaatc ttgaactgac ggtgagcagg gaagaggcca agaaaagttg
       61 gcgttttctc gtcgggagtg tgtttggagg gattgcaaat gaatcgtaga ctgtcgatga
      121 tgttgttgct cttcggactc tctctcgcgg tcatccttct cgggcgttcc gtgtgggctt
      181 ccgaaaaacc gtggctgacc ttggaaaagg cgctcgaaat ggcggaacgc aacaatccgc
      241 tcatctccgc ctccggcgag aggatagcgc aggcgcgagc gcgcctggac cgggcgtcgg
      301 cggcgtcgtt gccgcagctc ggcgtgtcgc ttctgtatca ggaggtgcag aacgaacctc
      361 gttaccccgt cgttcctgcg ggatacgcga aggcgggctt cgagagcact tggaaggcgg
      421 cgcttacgct gaactggttg ctctacagcg ggggagccgt ccgcctgaac acggaatcga
      481 agaggatcgc ccttttcgga gtggaagcgc gggaaaaacg cacgcgtcag actgtcctct
      541 ggggggttgc ggcggcatgg tacgaccttc ggcgcgccga ggggaagctg accgttgccg
      601 aggatgtgtt cgcgcttaca aaggagcatc tccagcatgt cgaggcgctc ttccggaacg
      661 ggatgacggc gaagaacgaa gtgcttcggg tgcaggtctc cgtttcggag gcggaactga
      721 acctcattag ggcggagaac gccgttgcgg tagagtggaa ggcgttggag cgagcggtcg
      781 gcgtcccgct ctgcggcgcg ttcgatcttc cgccgcgcga agagagcccg gagctcttct
      841 ccatgccgca ggacgccgtt tcggtcggac tcgcccatcg cccggagatg gaagcgcttg
      901 acctggcggc gcgctcggcg tcgcttgcgt cacaagcggc gaacgcgcag ggaggaccgc
      961 aggtcgcgct tgtgggagaa gtttatgcgg cggacgatga gttttttccg cagaaacaga
     1021 acgattggaa ggtaactttg ctggcctcct ggagcttttt cgacggaggc gcttcgtcag
     1081 ccagagcccg cgaggccagg gcggccgcgg aagagtttct ctttaggatg gaggatatga
     1141 agcggcagat cgaacttgag atctccgtcg cccggctgaa ctgtgaatcg tcgctccagc
     1201 ggatcagcac ggggcggacg atggtcgaga gcgcggagga ggactacaga atggcgctgc
     1261 ggcgctacac ggcacaggtg ggaacgaata tcgatgtgct cgatgcgcgc gttgccctct
     1321 ccaatgcccg gaaccaactt gtggagagcg tctatgactc gctcaaggcg agggcggaga
     1381 tggagtacgc tatgggcgtc atcggaagcg acggaagagg ggaggaacgg actaaatgaa
     1441 aaaatacctg gtattttttc tcttcttagc tgcgttcggc ggtcttatac tctaccggcg
     1501 gcacgagacc ctctcgacga tgacggaagt cccggtg
//
LOCUS       DAOPKU010000677         1534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_97630_length_1534_cov_2.455713, whole genome shotgun sequence.
ACCESSION   DAOPKU010000677 DAOPKU010000000
VERSION     DAOPKU010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97630_length_1534_cov_2.455713"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1534)
                     /locus_tag="PK849_14870"
     CDS             complement(<1..>1534)
                     /locus_tag="PK849_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPE67463.1"
                     /translation="GWLTSMDAGEGERAYRLNADQIVWKNGDGSVPFDPVFRPNSRRQ
                     ERKPNAFFTDLYMRAAYTGRIKSREHTGQTDKKSRQQREEDFREGKIAALFCSPTMEL
                     GIDIADLNVVHMRNVPPNTANYAQRSGRAGRSGQPALVFAICSRQSAHDTHFFNNRDR
                     MVAGTVASPRLDLSNEELLRSHFHALYLSSRGLTGLGANLHELLDYDDPSLPLKPEVR
                     GDLSEAVQRGGIVLEKWGQVVADLQAAGRIAEPLETGARWLAGMKDSFDASLNRWRNL
                     YRQHKEQVLAARKIIDNPAFKMTSPEYREAQRNEMLAHRLRALLMNEVSSDSVSEFYS
                     YRYLASEGFLPGYNFTRLPIRLFLDVGKGTDAISRDRVLAIREMGPENLVYHNGSKYK
                     VTRAQIRETADETQEATVCLDSGYILFDGEQGLNTDPWSGAGLDGRTLRIAGLLPLPD
                     ALAERTKHITCDEEERQRIGYQIDTYFRFKGDEKRLSEIRLVAGEDVLLRLRFIPSAE
                     LIY"
BASE COUNT          232 a          458 c          513 g          331 t
ORIGIN      
        1 cgtagatcag ctcggcggag ggtatgaagc ggaggcgaag cagaacgtcc tctccggcga
       61 cgaggcggat ttcggagagg cgcttctcgt cccccttgaa gcggaagtag gtgtcgatct
      121 ggtagcctat ccgctgccgc tcctcctcgt cgcaggtgat gtgtttggtg cgttccgcaa
      181 gggcgtccgg cagaggaaga agccccgcta tccggagagt gcgtccgtcg agtccggcgc
      241 cgctccacgg gtcggtgttc agcccttgct cgccgtcgaa gaggatgtag ccggaatcca
      301 gacagacggt cgcttcctgc gtttcgtccg ccgtctcgcg tatctgggcg cgggtgacct
      361 tgtacttcga tccgttgtgg tagacgaggt tttccggtcc catctcgcgg atggcgagca
      421 cacgatcccg gctgatcgcg tcggttcctt ttccgacgtc gaggaagaga cggataggaa
      481 ggcgggtgaa gttgtatccg ggcagaaatc cttcgctcgc gagatagcgg tagctgtaga
      541 actcggagac cgaatccgat gacacttcgt tcatcaggag agcccgcagg cggtgggcga
      601 gcatttcgtt gcgctgcgcc tccctgtatt ccgggctcgt catcttgaac gccgggttgt
      661 cgatgatctt tctcgcggcc agcacctgct ccttatgctg gcgatagagg tttcgccacc
      721 ggttcagcga ggcgtcgaag gagtccttca ttccggcgag ccaccgcgcc cccgtttcca
      781 ggggctccgc gattcgaccg gcggcttgga gatccgcgac gacttgtccc cacttctcca
      841 gcacgattcc accccgctgt acggcctcgg agagatctcc ccggacttcc ggtttgaggg
      901 gcagacttgg atcgtcgtag tcgagcagct cgtggagatt ggcgccgagc cccgtcagtc
      961 cgcgggacga gaggtagagc gcgtggaagt ggctgcggag cagctcctcg ttggagaggt
     1021 cgagacgcgg cgaggcgacc gttccggcga ccatccggtc gcggttgttg aagaagtgcg
     1081 tgtcgtgcgc ggactgtctg ctgcagatgg cgaagaccag ggcgggctgt ccgctcctcc
     1141 cggcgcgccc cgaacgctgg gcgtagttcg cggtgttggg gggcacgttc cgcatgtgga
     1201 cgacgttgag gtccgcgatg tcgatgccga gttccatcgt cggggaacag aagagcgccg
     1261 cgatcttccc ctcccggaag tcctcctccc tctgttgtct gctcttcttg tccgtctgtc
     1321 ccgtgtgttc cctcgatttg atccgtccgg tgtaggcggc gcgcatatag agatcggtga
     1381 agaatgcgtt cggtttccgc tcctgcctgc ggctgttcgg ccggaagacg gggtcgaagg
     1441 ggacgcttcc gtcgccgttt ttccagacta tctggtcggc gttcagacgg taggcgcgct
     1501 cgccttcgcc cgcatccatg cttgtaagcc agcc
//
LOCUS       DAOPKU010000678         1533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_97664_length_1533_cov_3.916779, whole genome shotgun sequence.
ACCESSION   DAOPKU010000678 DAOPKU010000000
VERSION     DAOPKU010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97664_length_1533_cov_3.916779"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1192)
                     /locus_tag="PK849_14875"
     CDS             complement(119..1192)
                     /locus_tag="PK849_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67464.1"
                     /translation="MVAALCLSKKTSLDEWGSFEFGKAARAYSRARRFREWLRNPRID
                     PGFLLHPLTERLISKAGKHVHIALDTSILFNAFCMVRVSLIFLDRAVPLVWSVRKSKS
                     ASVPFSAYRGLLEEVAAMIPSGTQPIFLADRGFQNASLFELLKQLGWWCRIRAKSDAP
                     VRTRKGEAIIPGKLCLKRGLPRFWRNASYGGVGGVTFGALLPMAAGEEPWYIVSFGGD
                     FTDIFRDYSKRFCIEEEFLDEKSGCFGLSKSRIRDEKRIERLLCVIATAAVLMVELGI
                     ETERAGERVNIDTHGKRGLSYLKIGLRKLMQILHFPKLLPIWIRLSPLPFDYEPEGVS
                     VSRKEDEETARRKRKCEVYEVYD"
BASE COUNT          317 a          438 c          431 g          347 t
ORIGIN      
        1 tttatgatca tgggcatgct tgttcttgga ggagctctgg cgtttctcgc ccgaatgggc
       61 gtggacgaac ctgactgacg gaggaatctt cttctcttca ccgtaataaa gcgcatcgtc
      121 aatcgtacac ctcgtaaact tcacacttgc gtttccggcg ggcggtctct tcgtcctctt
      181 tacgggagac cgaaacacct tccggctcgt agtcgaaagg aagcggggag agtcgaatcc
      241 atatcgggag gagcttcggg aaatggagta tctgcatcag ctttctcaat ccgatcttga
      301 ggtagctcaa accacgcttc ccgtgagtat cgatgttcac gcgctctccc gcccgctccg
      361 tctcgattcc gagttccacc atcagaacgg cggccgtcgc gatcacgcac agcagccgct
      421 cgatcctctt ctcgtcgcgt atccggctct tcgaaagacc gaaacacccg ctcttctcgt
      481 cgagaaactc ctcttcgatg cagaagcgtt tcgagtagtc gcggaagatg tccgtgaagt
      541 cccctccgaa ggagacgatg taccacggct cttcgcctgc ggccatcggc agcagcgcgc
      601 cgaacgtcac gccgccgact ccgccgtagg aggcgtttct ccagaatctg gggagtccgc
      661 gcttcagaca gagtttcccc ggtatgatcg cctccccctt cctcgtccgt acgggagcgt
      721 cgctcttcgc tcttattctg caccaccagc cgagctgttt cagaagctcg aagagagagg
      781 cgttctggaa tcccctgtcc gcgagaaaga tcggctgcgt ccccgacgga atcatggccg
      841 cgacctcctc caggagcccc cgatacgcgg agaagggaac cgaggcgctc ttgctcttcc
      901 gcacggacca gaccagcgga accgccctgt cgagaaagat cagggagacg cgaaccatgc
      961 agaatgcgtt gaaaaggatg gacgtgtcca gagcgatgtg gacgtgtttt cccgctttcg
     1021 atatcaggcg ctccgtaaga gggtgaagaa ggaacccggg atcgatcctc ggattgcgga
     1081 gccattcgcg gaaacgcctc gccctgctgt atgcgcgcgc ggctttgccg aactcgaagg
     1141 aaccccattc gtcgagactc gtctttttcg acaggcagag agctgccacc atcgcgccga
     1201 gcgtttctat gagccttcgg tcgagaaacg gagcgaggtg ttgataaagg aggcggatga
     1261 ttgtagtatg tagaggcggc ctgttgatga tcgattcccc cgttctgtat agtttcggcg
     1321 aacgtattga acacgggaaa cgatgaattg tctacaggcc gttctttttt caaggtgctt
     1381 tattgttccg cactgtcgcc gggacgaaac ggaggacatt caatccatac aatccatact
     1441 ttatcatggt gaattggaaa agattcctcc gtcagtcagg aaggcggggc tcttcgcggg
     1501 atatgtgcat tcctcggttg tgctgtatgt agg
//
LOCUS       DAOPKU010000679         1529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_98067_length_1529_cov_3.670285, whole genome shotgun sequence.
ACCESSION   DAOPKU010000679 DAOPKU010000000
VERSION     DAOPKU010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98067_length_1529_cov_3.670285"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..682
                     /locus_tag="PK849_14880"
     CDS             224..682
                     /locus_tag="PK849_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67465.1"
                     /translation="MTDTFDLGLASDLWEYWGFSSWEAPGLKGVYRRVTFVKNALLGE
                     VCRYYADDFIIWSHSGRSDKIRILESCRPQPDLMTQRYLFVEENDSGEKGRIRSFLFG
                     LRGYAEVHSFTPGGRYAKRMKDLAPLVDKALELVRSRKSGSGGGEQGELQ"
     gene            697..>1529
                     /locus_tag="PK849_14885"
     CDS             697..>1529
                     /locus_tag="PK849_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HPE67466.1"
                     /translation="MVFIFSSEVNEVLSTLAILFLIAVVLFVLCYKIYGSYMANIYGL
                     NDDVQTPAEALFDGIDYCPAHPAVLLGHHFASIAGAGPIVGPITAAAMFGWLPAYLWC
                     LIGSAFLGGPHDMGALVASMRHDGKSVGEVVDKWIGRKGKVLFLCFTILALVLVVAVF
                     LQLSAGSFAADPAVAFSATLYIFMALLFGVLIYKYHVPLWLMTVVMVPIVIYACWYGN
                     DAAWVAEMFKLPMETWRWILAGYIFFASVLPVWLLLQPRDYLASYFLYFAVIIGTIGM
                     L"
BASE COUNT          305 a          355 c          409 g          460 t
ORIGIN      
        1 agtgacctct tccgtgtgaa ggaagcgcta cttccgctga gctaatcgcc cgaaacgggg
       61 gatattatag cgtgcatctg tcaagtttgc aagtaaaatt gcgcttggaa ttttctctct
      121 tttggtgctg aaatgagggg tgaaaaaaaa ataaaaggaa actaaaaatc acttcccatt
      181 ttgcagcatt ggagtataat cattccgaag gggagatgag ctggtgaccg atacttttga
      241 tttgggactc gcttccgatc tgtgggagta ctggggattc tcttcttggg aggctcccgg
      301 gttgaaggga gtatacagaa gagtaacctt tgtgaaaaat gcgcttttgg gagaagtttg
      361 tcggtattat gccgatgatt ttatcatttg gagccatagc ggcagaagcg ataaaatacg
      421 tattttggaa tcgtgccgtc ctcaacccga cctgatgacg cagcgctacc tttttgtcga
      481 ggaaaacgat tcgggagaga aaggaagaat acgttctttt ctctttgggc tgagggggta
      541 tgcggaagtt cattcattta cacccggagg gcggtatgcg aaacgcatga aggatcttgc
      601 tcctctggtt gacaaggctc tggagcttgt gcgctccagg aagagcggtt cagggggtgg
      661 tgagcaggga gaacttcaat aagagtaaat tgcagcatgg tttttatttt ctcatcggag
      721 gtgaacgaag tgctttctac tcttgctatt ctgtttctga ttgctgtcgt tttatttgta
      781 ctgtgctaca agatttatgg atcctacatg gcgaatatct atggtttgaa tgatgacgtc
      841 cagactcccg ccgaagcgtt gtttgacgga atcgactact gcccggccca tcctgcggtg
      901 cttttgggcc atcacttcgc gtccattgcc ggcgccggtc ccattgtcgg ccccattaca
      961 gcagccgcta tgttcggctg gcttcccgcc tacctgtggt gtctcatcgg ttccgccttc
     1021 cttggcggcc cgcatgacat gggagcgctt gtcgcttcga tgcgccacga cggaaagtca
     1081 gtcggcgaag tggtagacaa gtggattggc cgcaaaggca aggttctctt cctttgcttt
     1141 accattcttg ctcttgttct tgttgtcgcc gtctttttgc agctttccgc cggttctttc
     1201 gccgcggatc ccgccgttgc attttctgcc actctgtata ttttcatggc attgcttttc
     1261 ggcgtgctca tctacaagta tcacgttccc ctgtggctca tgacagtcgt catggttccg
     1321 attgttattt acgcctgctg gtacggcaac gatgccgcgt gggtcgcgga aatgttcaag
     1381 cttcctatgg agacgtggcg ctggattctt gccgggtata ttttctttgc ctctgttctg
     1441 ccggtatggc ttcttcttca gcctcgcgac tatctggcat cctatttcct ttatttcgcc
     1501 gtcatcatcg gaacgatcgg catgctgat
//
LOCUS       DAOPKU010000680         1529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_98072_length_1529_cov_3.496608, whole genome shotgun sequence.
ACCESSION   DAOPKU010000680 DAOPKU010000000
VERSION     DAOPKU010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98072_length_1529_cov_3.496608"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK849_14890"
     CDS             complement(<1..186)
                     /locus_tag="PK849_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67467.1"
                     /translation="MAKPIVARWEWRTFGAGFEEPEARIRQFEQGNYKESEEVYVLSR
                     NSDENVKVRDDLLDIKSL"
     gene            complement(428..1474)
                     /locus_tag="PK849_14895"
     CDS             complement(428..1474)
                     /locus_tag="PK849_14895"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPE67468.1"
                     /translation="MTRFNDRHELLENLQRLLSESRRAALKAVNAEMLRAYWEMGKAL
                     SQWEETHGGSPDLLEGVAADLASDFGKAFSGKKLAAILKFFKEYENRPPLFRFREESD
                     LAESGLRSLDPGLSWSHYTVMMDIEDSAVRDEVERKVSREGWTASELEKHLRAAGEKT
                     SDVTDDMPLFEERTPPSPEDYVVDPHVQAFMGIPDRQRLTESILERALLDHLQRFLLE
                     SGKELYLVERQKKMELKGGARYIDLVFYSRSLHAFFLVELVCGDFSSEDEEKLSLVLE
                     YMSRESALSDENPPAALLIAVSRTAGRMKYLFASKERELEYRRLLPSEDQLAVEIQRE
                     QVDILKRYPHLEDE"
BASE COUNT          343 a          452 c          353 g          381 t
ORIGIN      
        1 aagcgacttg atgtccaata gatcatcccg caccttcacg ttctcgtcgc tgttccggga
       61 gagcacgtag acctcctcgc tctctttata attcccctgt tcaaactgcc ggatccttgc
      121 ctcgggctct tcgaatcccg ccccgaacgt cctccactcc caacgcgcca ctataggttt
      181 tgccatgttg tcccctccat gttctgtatg aaatagtata gataaaaaaa tcggaggagc
      241 attcgctccc ccgacaaaaa atatacaata accctactgg ggcaaaacac cctgaacgca
      301 tacgatgcgc agaatattct gctttttcaa ggtccctgtg tattcactat atcgcttctc
      361 ggcatctttt gtcaagccag gattcgcaca ttttgtgtat tttcttcgcc tcagccccga
      421 aaggacgcta ctcatcctcc aggtgaggat atcgcttcag tatatcgacc tgttccctct
      481 gaatttcgac ggcaagctga tcctcagagg ggagcaaacg tctgtactcc agctcccgct
      541 ctttcgacgc gaagagatac ttcattctcc cggcggttcg agatacggcg ataagcaacg
      601 ccgcaggagg attttcgtcg gacagagcgc tttctcgcga catatactca aggacgagcg
      661 acagtttttc ttcgtcttca ctcgaaaaat ctccgcagac taactctacc agaaaaaacg
      721 catgaagaga acgcgagtag aagacaaggt cgatatagcg cgcccctccc ttgagctcca
      781 tctttttctg acgttccacc aggtagagct ctttgccgga ttcaagaaga aaccgctgaa
      841 ggtgatcgag cagcgccctt tctagaatgc tctctgtcag ccgctgacgg tcgggtattc
      901 ccatgaacgc ctgaacgtgg ggatcgacaa cgtagtcttc cggtgaagga ggagtcctct
      961 cctcgaagag cggcatatcg tcggtcacgt cggaggtttt ttctccggcg gccctcagat
     1021 gcttttccag ttcggacgcc gtccatcctt cgcgcgatac ctttcgttcg acttcgtccc
     1081 tgacggccga atcttcgata tccatcatca ccgtgtagtg gctccagctc agtccgggat
     1141 caagggagcg aaggccggat tcggcgagat cgctctcctc cctgaaacga aagagcggag
     1201 gacggttttc gtattccttg aaaaacttca ggatagcggc aagtttcttt ccggaaaacg
     1261 ctttgccgaa atccgacgca agatccgcag ccactccttc aagaaggtcg ggagagcctc
     1321 cgtgcgtttc ctcccattgg gaaagcgcct ttcccatctc ccagtacgca cgaagcatct
     1381 cggcgttcac tgccttcaaa gcagcccttc gagactctga aagcagccgt tgcagatttt
     1441 ccaacaactc gtgccggtca ttgaaacgtg tcattctctc agcctccttt tttggattag
     1501 gccagactcc cttgcagggt tcggctcag
//
LOCUS       DAOPKU010000681         1517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_99217_length_1517_cov_3.917921, whole genome shotgun sequence.
ACCESSION   DAOPKU010000681 DAOPKU010000000
VERSION     DAOPKU010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99217_length_1517_cov_3.917921"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK849_14900"
     CDS             <1..514
                     /locus_tag="PK849_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HPE67469.1"
                     /translation="SYQGLVIANKQSGVGALEQLKGKRFAFVDPKSGSGYLYPRLLLQ
                     EKGFDPDHFFGEILFLGSHDKVIQAVFEGNVDGGATYTDAWDDAAKTMDLRRLEILAR
                     TEDIPKDALAVPASVDAAFAAALAETFLGMKSEGGESGAVMKKVSIDAFVKADDAQYN
                     VLRRAKAMNR"
     gene            complement(573..1316)
                     /locus_tag="PK849_14905"
     CDS             complement(573..1316)
                     /locus_tag="PK849_14905"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE67470.1"
                     /translation="MKRQNLRKALMVIFVLSLPVTMFYLSPLLTLQGAWKGIVTSSLV
                     FVGLSAVLAFFAGRVFCGWICPGGAVQEVLFPANDRRVPGGKLDLIKYGIAAVWLALL
                     VAFFIRAGGLLSVQPLYGIESGFSLATPVSYVMFYVAMGGMVVLSLAVGRRPFCHYGC
                     MLAPFMIAGKYAAKKIGSPRLHLEAESEKCIDCKLCTKNCPMSLDVNAMVRSSEMDSP
                     ECISCAACVDVCPRQVISLVFSSTGSKKS"
BASE COUNT          338 a          438 c          445 g          296 t
ORIGIN      
        1 atcctaccag gggcttgtga ttgccaacaa gcagagcggc gtcggcgccc ttgagcagct
       61 caagggaaaa cggttcgctt tcgtcgatcc gaaaagcggc tcggggtacc tctatccgcg
      121 tttgctgttg caggagaagg ggttcgatcc ggaccacttc ttcggagaga ttcttttcct
      181 cggaagccat gacaaggtca tccaggccgt ttttgagggg aacgtcgacg gcggggccac
      241 ctacaccgat gcctgggatg atgcggcgaa aacaatggat ctccgtcggc tggagatcct
      301 cgccagaaca gaggatatcc cgaaggatgc gctcgccgtt cccgcttccg tcgatgcggc
      361 gtttgccgcc gctcttgcgg aaacgtttct tggaatgaaa tccgaagggg gagagtccgg
      421 cgccgtgatg aagaaggtct ccatcgacgc ttttgtgaag gccgacgacg cgcagtacaa
      481 tgtgcttcgc agggcgaagg cgatgaaccg ctgaaaaaag aagatcatgc ggggcgtacg
      541 ccgggtttct ccgaagcgcg ccccgtatcg tgtcagctct tctttgatcc cgtcgaggaa
      601 aaaacgagtg aaataacctg tctcggacag acgtcgacgc acgccgcgca cgagatgcac
      661 tccggggagt ccatttcgga ggagcggacc atcgcgttca cgtccaggct catggggcag
      721 ttcttcgtgc agagcttgca gtcgatgcac ttttcgctct cggcctcgag gtgcaggcgt
      781 ggggagccga tctttttcgc tgcgtacttc cccgcgatca tgaagggcgc cagcatgcag
      841 ccgtagtgac agaacggccg ccgccccacg gcgagagaaa ggacgaccat gccgcccatc
      901 gcgacgtaga acataacgta cgataccggc gtcgccagag agaacccgct ttcaatgccg
      961 tagagaggct gcactgagag cagtccccct gcacggatga aaaaggcgac gaggagagca
     1021 agccagacgg cggcgatgcc gtacttgata aggtcgagtt tcccgcccgg aaccctccgg
     1081 tcgttcgctg gaaagagcac ctcctgcact gcgccccccg ggcagatcca gccgcagaac
     1141 acccttccgg cgaagaaggc gagaacagcc gataggccta cgaagacaag gctcgatgtc
     1201 acaatgccct tccaagctcc ctgcagcgtg aggagcgggg agaggtagaa catcgtcaca
     1261 gggagcgaga gcacaaaaat caccatgaga gctttccgca gattctgccg tttcattgcg
     1321 atccctccgt cattgaattg ttcactatta tagcagatca tacctaatgg gggtattctt
     1381 atttcccccg tgaagacatc gttccgtcgc acgccggcgc atactcttcc gcatcgtgat
     1441 cggatactgt tcgcaccttt cacccatgac ctttttgcgc tcagagcgta caatagtata
     1501 aatcaccccg gtgcgag
//
LOCUS       DAOPKU010000682         1510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_99905_length_1510_cov_2.897595, whole genome shotgun sequence.
ACCESSION   DAOPKU010000682 DAOPKU010000000
VERSION     DAOPKU010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99905_length_1510_cov_2.897595"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1403
                     /locus_tag="PK849_14910"
     CDS             150..1403
                     /locus_tag="PK849_14910"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE67471.1"
                     /translation="MLLIFLGVPIAFVIGIISLAGIATLPFVPNLTVFMKMFNGLNSF
                     VLLAVPLFILAANLMNHGQITRKLVNFCIALVGNIRGGLAHANILVSMIFAGVSGSSQ
                     ADTAGVGKMLIPAMIESGYDKENSVGVTAASSTIGVIIPPSIPMVIYSGLTNASVAAL
                     FLGGMIPGILVGVTMMMIVYLIGHRRNFPKYEKAKYSDVWKLFKESFPALLTPVIIIG
                     GITTGWYTPTEAAAFASVYALLISLFIYRTLKLRDLPGILKETLKLSSLSLFALATAS
                     ALGELLGYYKASEGVAAFFAANVGNPRIFLLIVIAFFLFVGTFMDAIPAMILFIPVIL
                     PTAVTMGISPVHLGVVVVMTLAVGLVTPPYGLCLLIAGSIADLSIERSLKGVLPYISA
                     LMIILLLVAMFPDVAFFVPKALGVM"
BASE COUNT          258 a          499 c          397 g          356 t
ORIGIN      
        1 ctgggtcaag atggggtaca cgtggctctg cctccccatc accggatgca cgatgacggt
       61 ctacactgtc gcgcacatca ttgatgacat acgtattctc tcctcgaagg aggccgtgtc
      121 atgacgccga tgctgatcgt cggttcttcg tgctgctcat cttcctcggc gtgcctatcg
      181 ccttcgtcat cggcattatc tcgcttgcgg ggatcgcgac gctgccgttc gtccccaatc
      241 tcaccgtctt catgaagatg ttcaacggcc tcaattcatt cgtcctgctc gccgtgccgc
      301 tcttcatcct ggcggccaac ctgatgaacc acggacagat cacccggaag ctggtgaact
      361 tctgcatcgc gcttgtgggg aacatccgcg gcggcctcgc gcacgcgaat atcctcgtgt
      421 ccatgatctt cgccggagtc tccggctcct cccaggcgga cacggcgggc gtcgggaaga
      481 tgctgattcc cgcaatgatc gagagcgggt acgacaagga gaactccgtc ggtgtcaccg
      541 cggcgtcctc gaccatcggc gtcatcatcc cgccgagcat cccgatggtg atctactccg
      601 gcctcaccaa cgcctctgtc gcggcgctct ttctcggagg gatgatcccc ggcatcctcg
      661 tcggcgtcac catgatgatg atcgtctacc ttatcggcca ccggcggaac ttcccgaagt
      721 acgagaaggc gaagtactcc gacgtctgga agctcttcaa ggaatccttc ccggcgctgc
      781 tgacccccgt gattatcatc ggagggatca ccacaggatg gtacacgccc acggaggcag
      841 cagccttcgc ctccgtctac gcgctcctca tcagcctttt tatatacagg acgctgaaac
      901 tgcgcgacct acccggaatc ctgaaggaga cgctcaagct cagctcactt tcactcttcg
      961 cgctcgcaac agccagcgct ctcggcgaac tgctgggcta ttacaaggcg tccgaaggcg
     1021 tcgcggcttt cttcgcggcc aacgtcggca atcctcgtat cttcctcctg atcgtgatcg
     1081 ccttcttcct cttcgtcggc accttcatgg acgcaatacc ggcgatgatt ctcttcatcc
     1141 cggtgatcct gccgacagcc gttaccatgg gcatctctcc cgtccatctc ggcgtcgtgg
     1201 tcgtcatgac gctcgcggtc ggtcttgtga cacccccgta cgggctctgt ctgctcatcg
     1261 cgggttccat cgcagatctg tctatcgaac gttctctgaa aggcgtcctt ccctacatct
     1321 ccgcgctgat gatcattttg ttgctggtgg caatgttccc cgacgtcgcg ttcttcgttc
     1381 cgaaagctct cggcgtgatg tagtccccga atgcgaagag agggaggcgg gaaaattccg
     1441 tcctccctct ctttttgtag ttcgcggcgc ccggcaggaa aatactattc gtaccggcgg
     1501 gcctcttccg
//
LOCUS       DAOPKU010000683         1502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_100635_length_1502_cov_4.421562, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000683 DAOPKU010000000
VERSION     DAOPKU010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100635_length_1502_cov_4.421562"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1502)
                     /locus_tag="PK849_14915"
     CDS             complement(<1..>1502)
                     /locus_tag="PK849_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPE67472.1"
                     /translation="LLFEPVKIESSAETYELLWKEVASASGAQPLPSWQTLHLRLASW
                     PTLRISSGVAYARDTLVLEEAPLFKGNLERFQWMCDAWRRDGYHIRLFTANPRLSSLY
                     DDDLSVIEGALSKGFVDPLKRIVHISDLELSGISDRVASAGTVFAPPREWSEQLVEGR
                     LLIHEEYGLCVFRGSEEVAISGEVMDSLVLEFADNKRLFLPVLQLHKITPLSEHADDD
                     VQLDSLRGTRWKKSVAKTKERVLQEVRALLELYAKRELVEGFAFPPRDDLFDQFERSF
                     PHAETKDQLVAISEILEDMERRFPMDRLLVGDVGYGKTEVALRAAFRAVQGGKQAALL
                     VPTTILAQQHYLSFTARLTGFPVRVAFLSRFLSRKEQKDVLERVAAGEVDILIGTVRM
                     LREDVAFKDLGLLIIDEEHRFGVMSKEKLKQARENVDVLMLSATPIPRTLALSLKGLR
                     SISVLNEAPHNRIPVVTTAGPWSASIVKGAVSRELERGGQVYYVTNRNTR"
BASE COUNT          302 a          436 c          473 g          291 t
ORIGIN      
        1 tccgcgtatt ccggttggtg acgtagtaga cctgtcctcc cctttcgagc tccctggaaa
       61 ccgctccctt cacgatcgag gcgctccatg gaccggcggt ggtgactacg ggaatccggt
      121 tgtgcggggc ctcgttgagc acggagatgc tcctgaggcc cttgagcgag agcgcgagcg
      181 tccggggaat gggcgtggcg gagagcatga gcacgtcgac gttctccctg gcctgtttga
      241 gcttttcctt ggacatgacg ccgaaacgat gctcttcgtc gatgatgagc agcccgaggt
      301 ctttgaacgc gacatcctcc ctgagcatac gcaccgtccc tatgaggatg tccacctcgc
      361 ccgcggcaac gcgctcgagc acgtccttct gctccttgcg cgagagaaaa cgcgagagaa
      421 aggcaacccg gaccggaaaa ccggtcaagc gcgcagtaaa cgagagatag tgctgctgcg
      481 ccagtatggt ggtgggcacg aggagcgccg cttgcttccc gccctggact gcgcggaagg
      541 ccgcccgcag tgcgacttcg gtctttccgt atccgacgtc gccgacgagc agtctgtcca
      601 tgggaaagcg ccgctccata tcctccagaa tttcggagat ggccacgagc tgatcttttg
      661 tctccgcatg gggaaaggag cgttcaaact ggtcgaaaag atcgtcccgc ggaggaaacg
      721 cgaatccctc cacgagctcc cgcttcgcgt acagttcgag cagcgcccgc acctcctgca
      781 gaacacgctc tttcgttttg gcgacgctct tcttccacct ggtcccccgg agcgaatcaa
      841 gctgtacatc gtcgtcggca tgctcggaaa gcggcgtgat cttgtggagt tgcagcaccg
      901 gaaggaagag gcgcttgttg tcggcaaact ccagcacaag actgtccatg acctctccgg
      961 agattgcgac ctcctcggat cccctgaaaa cacaaaggcc gtactcctcg tggatgagaa
     1021 ggcgtccttc gacgagctgc tcgctccatt cccgcggcgg cgcgaaaacc gttccggcgg
     1081 aggctactct gtcggagatt ccggagagtt cgaggtcgga gatgtggaca atacgtttga
     1141 ggggatcgac aaaacccttg gagagcgctc cttcgatgac ggagagatcg tcgtcgtaga
     1201 gggaggagag ccgcgggttc gcggtgaaca gacgaatatg gtatccgtcg cgccgccacg
     1261 catcgcacat ccattggaac cgttcgagat ttcctttgaa gagaggggct tcttcgagta
     1321 caagcgtatc gcgggcgtag gccacgccgg aagagatgcg gagcgtaggc caggaagcca
     1381 gccggagatg gagcgtctgc caggagggaa gaggctgcgc tccggatgcg gaagctacct
     1441 ctttccatag gagctcgtag gtctcggcgg acgattcgat tttgaccggc tcgaagagga
     1501 ga
//
LOCUS       DAOPKU010000684         1494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_101440_length_1494_cov_4.504517, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000684 DAOPKU010000000
VERSION     DAOPKU010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101440_length_1494_cov_4.504517"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..968)
                     /locus_tag="PK849_14920"
     CDS             complement(3..968)
                     /locus_tag="PK849_14920"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HPE67473.1"
                     /translation="MKHYFAYGSNLSFAQMRERCPEHRKIGKGTLEGYKWFLSSRGYA
                     NVINSPEERVHGFVYGISEEDERNLDRFEEMHQGLYRKETLPVRLDEGGCLECLVYID
                     STSPASGEIVLKEWYKQRINRGIEDAELPPEYVRNVIRRFVPAEAKSEGGLPSTTMEG
                     LKALEKVLGCERLPMAREWGGAGLIVTRGEPEKPGNDWRFVRYAATPHAREVSWFIER
                     IRDAFYAEERVDNCSKYEFFGRLGNAAGRCGESVENADARMLCASVLREAYAMYEEME
                     QGRFLCLGIASGGDIADDFAERDFSEKEIRAFFENRGVDASVWKR"
     gene            complement(1025..>1494)
                     /locus_tag="PK849_14925"
     CDS             complement(1025..>1494)
                     /locus_tag="PK849_14925"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPE67474.1"
                     /translation="IDWEKLSTTDEDERKAGRYILREGDVLLSCRGTTVKSAVFRKQE
                     RPVIPSANLLVIRPDASVSGDFLRIFFESPSGDALIRSFQRGTVLMNLNHSDIMEMEM
                     PLPPMERQLEIVGRYFSERKKYEEATRRWEEEKERAYRELLEQVQDASAESGG"
BASE COUNT          247 a          491 c          396 g          360 t
ORIGIN      
        1 ttctaacgtt tccagaccga cgcgtccact cccctgtttt cgaagaaggc gcgaatctcc
       61 ttctccgaga agtctctttc ggcgaagtcg tcggctatat ctcccccgga ggcgattccg
      121 aggcagagga agcgcccctg ctccatctcc tcgtacatcg cgtacgcctc ccgaaggacc
      181 gaggcgcaga gcatacgggc gtccgcgttt tcgacgctct ccccgcacct gcccgcggcg
      241 ttcccgagtc tgccgaagaa ctcgtacttc gagcagttgt ccaccctctc ctcggcgtag
      301 aaggcgtccc ggatcctctc gatgaaccac gagacctccc tcgcgtgcgg cgtcgccgcg
      361 taccgcacga aacgccagtc gtttcctggt ttttcgggct cccccctcgt cacgataaga
      421 cccgctccgc cccactcgcg agccatcgga agcctttcgc acccgagaac tttttcgagg
      481 gctttcagtc cttccatggt cgtcgaaggc agtccacctt cggacttggc ttcggcgggg
      541 acgaatcttc ggatgacgtt tcggacgtac tccggcggca gctccgcatc ttctatcccc
      601 cggtttatcc gttgtttgta ccactccttt agaacgatct ctccggacgc cggggaggtg
      661 gagtcgatat agacgaggca ttcgaggcac cctccttcat cgagtctcac cggcagcgtc
      721 tccttccggt agagcccctg atgcatttcc tcgaaccggt cgagattccg ttcgtcctct
      781 tcgcttatcc cgtagacgaa gccgtgaacg cgctcctccg gcgaattgat cacgttggcg
      841 tacccgcggg aggagagaaa ccatttgtac ccttcaagcg ttccctttcc gattttcctg
      901 tgttcggggc atctctcccg catctgtgcg aaagagaggt tggatccgta ggcgaagtag
      961 tgtttcatgt gcatcatcct cccgtgtgtt ttttgacgga gttcgtccgg cagtcatttt
     1021 ttcttcaacc gccgctctcc gccgatgcgt cctgaacctg ttccagaagc tcccggtatg
     1081 cgcgttcctt ttcttcctcc cagcgccgcg tcgcctcctc gtactttttt ctttcggaga
     1141 agtaccgccc gacgatttcg agctgtcgtt ccatcggcgg cagcggcatc tccatctcca
     1201 tgatgtcgga gtggttcaaa ttcatcagga cggtccccct ctggaagctc cgtatcaacg
     1261 cgtcccccga agggctttcg aagaatatcc tgagaaaatc gccggagacg gatgcatcgg
     1321 gcctgatgac gagcaggttc gccgagggga tgacaggacg ctcctgcttc cggaagactg
     1381 ccgacttgac ggtggtccct ctgcaggaga ggaggacgtc gccttcgcgc agtatgtacc
     1441 tcccggcctt ccgttcgtcc tcgtcggtgg tggagagctt ctcccagtcg atct
//
LOCUS       DAOPKU010000685         1485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_102411_length_1485_cov_4.056643, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000685 DAOPKU010000000
VERSION     DAOPKU010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102411_length_1485_cov_4.056643"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..829
                     /locus_tag="PK849_14930"
     CDS             224..829
                     /locus_tag="PK849_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67475.1"
                     /translation="MAGKDHPPFSSPWEIQRFLSAIPYNSKEECKSPKRVLAERNAHC
                     FEGALFAASRLRELGYPPLLMDLRAWNDDDHVIALFKERGRWGAVAKSNFTTLRFREP
                     VYRTLRELAMSYFDFYFNTLGQKTLRAYSRPLKLSQFDEKNWTETEDDLEYIGDAFSK
                     LPYYELLDAEQVARLVEVDGDLLKAGLIGSNPAGLYVPEAE"
     gene            961..>1485
                     /locus_tag="PK849_14935"
     CDS             961..>1485
                     /locus_tag="PK849_14935"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPE67476.1"
                     /translation="MRCTGVSARGIRLPVLSKGADLVTIVADELVKASAACRDSFEFR
                     DGDIVGVTESVLARSQGNYVTLDDIAEDVRRRVPEGDVALIFPILSRNRFGRLLEGIA
                     RGVRGTLHVVLSYPADEVGNLLIDPRAFYLKSGQLPGDLFGEKEYDEIFGRYPHPFTG
                     VDYVSYYKDMDPKKI"
BASE COUNT          290 a          452 c          439 g          304 t
ORIGIN      
        1 actccccctt atattgctca tcttttgccg ggtgtacact tttttagatt ccttgcctgc
       61 cggttctgtt ctgcttgttc cctgtcctga tatgcgttat cattgtcgtg cagctgcacc
      121 gcactggaat ctccccttcg gacatctatc cggagggaag gacgcgcgtc cggcgcacac
      181 catgacaatc tcgattgtcg cggctggaag gtgaggagaa atcatggccg gaaaagacca
      241 ccccccgttc tcatccccct gggaaattca acgctttctc agcgccatcc cgtataactc
      301 caaggaggag tgcaaatcgc ccaagcgcgt gctcgccgag cgaaacgcgc actgcttcga
      361 aggcgcgctc ttcgccgcct cacgacttcg ggagctggga tatcctccgc tcctgatgga
      421 cctgcgggcg tggaacgacg acgaccacgt catcgccctc ttcaaggagc gcgggcgctg
      481 gggagcggtc gccaaatcca acttcaccac cctgcgtttc cgggagcccg tctaccgtac
      541 cctcagggaa ctcgccatgt cgtacttcga tttttacttc aacacgctgg ggcagaagac
      601 gcttcgggca tactccagac cgctgaaact gtcgcagttc gatgaaaaga actggaccga
      661 gaccgaggac gacctggaat acataggcga cgccttttca aaactccctt actatgaact
      721 gctggacgcg gaacaggtcg cgcgcctcgt cgaggtcgac ggggatctgc tgaaggccgg
      781 gctgataggc tcgaatcccg cagggctgta cgtcccggag gcggagtgaa attccgcgcc
      841 ttcgggcggc cgcgttgcag gaactgaaca cagaatgccg ggcggatggc gcccggcgta
      901 tattcgaggt tcgccgcttc cttgaggcgc ggcgagtcga aacggaagga atgaggttct
      961 atgcgctgta caggtgtttc cgcaagaggt atccgacttc cagtcctctc gaagggggcg
     1021 gatctggtca caatcgtcgc cgacgaactt gtcaaggcgt ctgcggcgtg ccgagattcg
     1081 ttcgaattcc gggacggcga tatcgtgggc gtgacggagt cggtgctcgc ccgttcgcag
     1141 gggaactacg tcacgttgga cgacatcgcc gaggatgttc gcagacgggt tcccgaaggg
     1201 gacgtcgcgc tgatcttccc cattttgagc cgcaaccgtt tcggacgcct gctcgaaggc
     1261 atcgcgcgag gagttcgcgg cacgctgcac gtcgtcctct cctaccctgc ggacgaggtg
     1321 ggcaatctgc tcatcgaccc gcgcgctttc tacctgaaga gcgggcagct ccccggagac
     1381 cttttcggcg agaaggagta cgacgagatc ttcggccgct acccccaccc cttcaccggc
     1441 gtggactacg tgagctacta taaggacatg gacccgaaga agata
//
LOCUS       DAOPKU010000686         1484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_102529_length_1484_cov_3.884535, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000686 DAOPKU010000000
VERSION     DAOPKU010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102529_length_1484_cov_3.884535"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..>1484
                     /locus_tag="PK849_14940"
     CDS             222..>1484
                     /locus_tag="PK849_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPE67477.1"
                     /translation="MTKSPRLGDILVLSGAISDEQLQSALKEQKSSGLKLGELLLHKK
                     IITEGKLMEALATQLRLQIYSLSRYRPMPEALRMVPQGVAKRLQVVPLAVEDGNTLLV
                     AMADPLDVLAQDEIRMISGMELRIGIVSPTELERNLERLYSIQESLEGANLEFMDSSP
                     RDVDLTQAMGATTPDDAPVIQLVNNIIEQAVRENCSDIHIEPFEKNTRVRYRIDGQLF
                     NALDFQKNLHPAVSSRLKIMSGMDISEKRKPQDGRILIKILGRRVDLRISSLPTIYGE
                     KIVIRILDQENAMVGLKKLGLEDDDKQKLDYICEAPYGIVLVTGPTGSGKSTTLYSVL
                     EQINKPEVNIVTVEDPVEYTLFGIGQVQVNERAGLTFNSTLRSILRQDPDKIMVGEIR
                     DGETAQLAIRAALTGHLVLSTLHTNDAPS"
BASE COUNT          367 a          460 c          367 g          290 t
ORIGIN      
        1 gagagcaggt cgccgccgga aatccttttg aaagaacaca ggaagccgtc cagagttaac
       61 cctcaggacg gcttcctgcg tattcgcatt ttgcttctat aagcgctgac aaatccggag
      121 cggttcttcc cctctcgttg tacattttcc ccttttacgc tattatcgtc ccatacaatc
      181 ccgcagcatc aaacatgtca tcgagataaa ggggagagag catgaccaaa tcgcctcgac
      241 tgggagatat tcttgtactg tccggagcaa tctccgacga gcagctgcaa tccgcgctca
      301 aggagcagaa aagttccggt ttgaagcttg gggagctgct gcttcataaa aaaatcatca
      361 ctgaaggcaa gttgatggaa gcgttggcga cgcagctcag gctccaaata tactcgcttt
      421 cccgctaccg gccgatgcct gaagcgttgc gaatggtacc gcaaggcgtg gcgaaacgtc
      481 ttcaagttgt tcctctcgcg gtcgaagacg ggaatacgct tctggtcgcc atggcggatc
      541 cccttgatgt tcttgcgcag gacgaaatcc ggatgatctc cgggatggag ctgcgcatcg
      601 gcatcgtctc gcccacggag ctggagcgaa accttgagcg tctgtactcc atacaggaat
      661 cgcttgaagg cgccaacctc gaattcatgg attcctcccc gcgcgacgtc gacctcacgc
      721 aggccatggg cgcaacaacc cccgacgatg ccccggtcat ccagctcgtc aacaatatca
      781 tcgaacaggc agtccgcgaa aactgttcgg acatccacat cgaacccttt gaaaagaaca
      841 cccgtgtgcg ctatcgaatc gacgggcagc tcttcaatgc cctcgacttt caaaaaaacc
      901 tccaccccgc cgtctcctcg cggctcaaga tcatgtccgg gatggacatc tccgagaagc
      961 gaaaacctca ggacggacga atactcataa aaatactcgg acgcagagtc gaccttcgta
     1021 tctcctcgct cccgaccatc tacggggaaa agatcgtcat ccgaatcctt gaccaggaga
     1081 acgccatggt cggcctcaaa aaactcggtc tggaagacga cgacaaacag aagctcgact
     1141 acatctgcga agctccctac ggcatcgtcc tcgtcacagg ccccaccgga agcggaaagt
     1201 ccactaccct ctattccgta ctcgaacaga tcaacaagcc ggaggtgaac atcgtcacag
     1261 tggaggaccc ggtggagtac acgctcttcg gtatcggaca ggtgcaggtc aatgaacgcg
     1321 ccgggctgac gttcaactcc acgctccgtt cgattctccg gcaggacccc gacaagataa
     1381 tggtcggcga aatccgcgac ggcgaaacgg cccagcttgc catacgcgca gccctcaccg
     1441 gacaccttgt cctctccacc ctgcacacca acgacgctcc aagc
//
LOCUS       DAOPKU010000687         1482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_102711_length_1482_cov_4.827610, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000687 DAOPKU010000000
VERSION     DAOPKU010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102711_length_1482_cov_4.827610"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1482)
                     /locus_tag="PK849_14945"
     CDS             complement(<1..>1482)
                     /locus_tag="PK849_14945"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPE67478.1"
                     /translation="KGTVEISLSVLAGTATNLGVFLPVAIMTGIAGQFLVQYAVTIVY
                     ATLFSLWVSLTLTPSMAARFKADAGIPRLGRFLCGWWDWIYSGFETLFLFLLRRAIRH
                     PVLTILVFAGLTFGVFKAGGFIGAEFMPRTDDGAMTINITLPSNTPVWVTQARAKEIE
                     DRIRETLPALKYIVSTVGASGRNQGTHKATIEAYFHNNVERPSTMDMADQLRPFVNAM
                     PGVDATVSGFRKGMGGGKPIQIIVRGDELETLYRIAETLRDRIKHVPGIVDAGIDVEM
                     GKPELQILPVRWRLSQFGINMTTLSQVVYGYLVGMDAGKFRQDGYEYDIKARINPEHA
                     KDIFKVPELPIMTEYGLVPLKEFSEVQWRDGPTEIVRKDRRKAVTVEADARYITVGEA
                     TENARRAIEGIELPPGYEIQLGGDAEFMAENFAELGQALLIAIVLTFIIVAAILESWF
                     FAFVITLSVPLAALGVIPAMLMSGVSISLFALIGLIMLVGLVVN"
BASE COUNT          295 a          480 c          435 g          272 t
ORIGIN      
        1 gttcaccacc aggccgacga gcatgatcag gccgatgagc gcgaagagcg aaatggaaac
       61 tcccgacatc agcatcgccg ggatgacgcc gagcgcggcc agcggcaccg agagcgtgat
      121 cacgaaggcg aagaaccacg actccagtat cgccgcgaca atgatgaagg tgagcacgat
      181 ggcaatcagc agggcctgcc cgagttcggc gaagttctcc gccataaact ccgcgtcgcc
      241 gccgagctgg atctcgtacc cgggaggcag ctcgataccc tcgattgcgc gccgggcgtt
      301 ttccgtcgct tcgcccactg tgatatagcg tgcgtcagcc tccacagtca ccgccttccg
      361 gcggtccttc ctgacaatct ccgtcgggcc gtcccgccac tgtacttcag agaactcttt
      421 cagcggcacg agcccgtact ccgtcatgat cggcagctcc ggcaccttga agatgtcctt
      481 cgcatgctcc ggattgatac gggccttgat gtcgtactcg tagccgtcct gccggaactt
      541 gcccgcgtcc atgccgacga ggtagccgta gaccacctgc gacagggtcg tcatgttgat
      601 gccgaactgg gagaggcgcc agcgcaccgg aaggatctga agctcgggtt tccccatctc
      661 cacgtcgata ccagcgtcga cgatgcccgg aacgtgcttt atcctgtcgc gaagcgtctc
      721 cgcgatacgg tacagcgtct ccagctcgtc tccccggacg atgatctgaa tgggttttcc
      781 gccgcccatc cccttgcgga atccgctgac tgtcgcatcc actccgggca tcgcgttcac
      841 gaaggggcgc agctggtcgg ccatatccat agtggacgga cgttcgacat tgttgtgaaa
      901 atacgcctcg atcgtcgcct tgtgcgttcc ctggtttcgt ccggacgcgc cgacggtgga
      961 aacgatatat ttcaacgccg gaagcgtttc cctgatccgg tcttcaattt ccttcgccct
     1021 cgcctgcgtc acccagaccg gagtattgct cggcagcgtg atattgatgg tcatcgctcc
     1081 gtcatccgtg cggggcatga attccgcgcc gatgaacccg cccgccttga agacgccgaa
     1141 cgtgagtccg gcgaagacga ggatcgtcag cacaggatgc cggatcgccc ttcggagcaa
     1201 gaagagaaag agcgtctcga agcccgagta aatccagtcc caccatccgc agaggaaacg
     1261 ccccaggcgg ggaattcccg cgtcggcctt gaagcgcgca gccatcgagg gagtgagcgt
     1321 cagcgagacc cacagggaga agagcgtcgc gtagacgatc gtcaccgcgt actgcacaag
     1381 gaactgcccc gcgatccccg tcatgatggc gaccggaagg aagacgccga ggttcgtcgc
     1441 cgtccccgcg aggacggaga gcgagatttc aaccgtcccc tt
//
LOCUS       DAOPKU010000688         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_103695_length_1473_cov_3.107898, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000688 DAOPKU010000000
VERSION     DAOPKU010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103695_length_1473_cov_3.107898"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PK849_14950"
     CDS             complement(<1..188)
                     /locus_tag="PK849_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67479.1"
                     /translation="MGRNSIEAFTNLRPHQRHPWHAFLCQLGAIALIDGGVEIPSVGK
                     NTPYSELPGVYDEEEWKEL"
     gene            complement(286..1239)
                     /locus_tag="PK849_14955"
     CDS             complement(286..1239)
                     /locus_tag="PK849_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67480.1"
                     /translation="MLRNSVASAVETLQAMEKQRAGENNNIFTIEGVSTLHHARFAPA
                     DRKRLDQEVELLFGKSVERRWPCVIVTTQTLEQSLDVDFDLLITDLCPADVLLQRIGR
                     LFRHDRRRPSAFSEPRCIVLVPDKGKDWLLKREAGKRQFGKERAYEDVRSVAATWELL
                     EDRIAEDGFLRIPEMNRYFVERSTHPAFLSRLAERLGPEWEQITGGIAGSKGAKRQRA
                     AFDIISWKKGYEAEFVSAADDHHIVTRLGLDDAVVFFQNPPVGPFGFSIEKMTVPGWM
                     LQGKDLSELDQGIEARQTEFGFEFSICGKSFRYSRYGLERS"
BASE COUNT          330 a          400 c          396 g          347 t
ORIGIN      
        1 agttccttcc actcttcttc atcgtacact ccaggaagct ccgagtacgg cgtgttctta
       61 ccgaccgagg gtatttctac tcctccgtcg ataagcgcga tagcgccaag ttggcagagg
      121 aatgcgtgcc aggggtgccg ctggtgggga cgcaggttgg tgaaggcttc gatactgttc
      181 ctccccaacg cggcaaacac cccaggcaag gtaagtttca cgcttgaaga gtctgtagtc
      241 cgcacttcga tacacggttc ttccaatata ttgtacaaaa tcgcatcagc tcctttccaa
      301 gccgtaccgg ctatagcgga atgatttccc gcatattgaa aactcgaacc cgaattccgt
      361 ttgcctcgct tcgatccctt ggtcgagttc cgaaagatcc tttccttgaa gcatccatcc
      421 tggaaccgtc atcttttcga tggaaaaccc gaaagggccg acgggtggat tctgaaagaa
      481 gactacggca tcgtcgagac cgaggcgagt cacgatgtga tggtcgtcgg ctgcggagac
      541 aaactccgcc tcgtatcctt ttttccagct gataatgtcg aaagcagccc gctgtctttt
      601 cgcacccttg cttcccgcga tgcccccggt tatctgctcc cactcgggtc cgaggcgttc
      661 agcgaggcga gacaaaaagg cgggatgggt cgaacgttcg acgaaatatc gattcatctc
      721 gggtatgcga aggaaaccgt cttccgcgat gcggtcttca agcagttccc acgtcgcggc
      781 gacgctccgg acgtcttcgt aggctctttc tttaccgaat tgacgctttc cggcctcccg
      841 cttgagtagc caatctttcc ccttatcggg aacgaggacg atgcagcgag gttcagaaaa
      901 cgctgaaggc cttcgacggt cgtgtcgaaa gaggcgtccg attcgctgaa gcaacacgtc
      961 cgcaggacag agatcggtga tcaggaggtc gaaatccaca tccaaggatt gctcaagcgt
     1021 ctgcgtcgtc acgatgacac agggccagcg tctttcgacc gattttccga agagcagctc
     1081 gacctcttga tcaagacgtt tccggtctgc gggagcaaat cgagcatgat gaagcgtgga
     1141 gacgccttct attgtgaata tattgttgtt ttctcctgca cgttgttttt ccatcgcttg
     1201 caaggtttcg actgcggagg caacggaatt ccgcagcaca agaatgcagc cccctgagtc
     1261 tgcggctcct agcgcaagtt gggcaagcgt ttcaggattc tccatcgaag gaaggagttc
     1321 caaacggacc tctttggctc tcccggaaag agtttttatc gcagtccgag cggatgtgga
     1381 ggatatcaga ggatacgggg ttccgatgca cagcgtttcg tccggaacgg gagaaactcc
     1441 aacggctttc cgagtacgaa aaatatgaag ata
//
LOCUS       DAOPKU010000689         1471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_103867_length_1471_cov_4.552260, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000689 DAOPKU010000000
VERSION     DAOPKU010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103867_length_1471_cov_4.552260"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PK849_14960"
     CDS             <1..414
                     /locus_tag="PK849_14960"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPE67481.1"
                     /translation="VRSDDGGFVAECSVDVVDYEIRVNGISLNHTTTDVNVDGTRYLV
                     ASISPANATNKRVRWVTSNWEIASVSQNGIVVGVRKGRAIISAITEDGGFVAECLVSV
                     NEGWLDFIEGCSALNSSPFSLLLLIPLLSLIGRRK"
     gene            611..>1471
                     /locus_tag="PK849_14965"
     CDS             611..>1471
                     /locus_tag="PK849_14965"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HPE67482.1"
                     /translation="MDRDTVDSVRRLAIDGPLLLQQIGLLGEVGKEPDGGRTRLALSG
                     QDKRARDLLVQWMKELGLDVKIDPIGNIFGILYGKGDSCADALIIGSHIDTVINAGPF
                     DGVYGVLSGLAVARAFRNAGVRPLRPLIVAAFTNEEGVRFQPDMMGSLFFTGGIPIDD
                     ALETKGIDGALLGQELRRIGYKGETEPSSLFPAEYLELHVEQGPVLDSKELDIGVVDN
                     LQGISWKRIEISGTANHAGTTPVSMRRDAGVAAARIIVFLRELALSSEGTRTTVGSLR
                     FYPDLINVIPS"
BASE COUNT          321 a          347 c          447 g          356 t
ORIGIN      
        1 gttcgttcgg atgacggagg ttttgttgcg gagtgttccg tcgatgttgt cgactacgag
       61 atcagggtga acggcatatc tctgaaccac acaacaacag atgttaatgt cgacggaact
      121 cggtatcttg tcgcctctat atcccctgcg aatgcgacga ataaacgggt tcgctgggtt
      181 acgtcgaact gggaaatagc gtccgtctcc cagaacggaa ttgttgtggg agtacgaaag
      241 ggaagggcga ttatcagcgc gattaccgaa gacggcggtt ttgtcgcgga gtgcctcgtg
      301 agcgtgaatg agggatggct ggattttatc gaaggctgtt cggccttgaa ctcgtcgcct
      361 ttttctctgt tgcttttgat tccccttctc tcgctgatcg gtcgacggaa gtaacgtaac
      421 tttgagaaca aggccttcgc tcttcgcaaa agaaacgcga agagcgaagg ccttctttgt
      481 actctcttgg agcgtctatg gatgcagtgt gaaaagagtg tgttctccag gaagttttag
      541 tgtatactgt ggaacacaat gagaccccga agagtattcc tcttgatccg gggaacaagg
      601 aggaaattcc gtggacagag atacagtcga tagcgtacgg cgccttgcga tcgacgggcc
      661 gctgcttctt caacagattg ggcttcttgg cgaggtcgga aaggaaccgg acggcggaag
      721 gactcgctta gcgctctcgg gtcaggacaa gcgcgcacgt gatctgttag tgcaatggat
      781 gaaggaactt ggacttgatg tgaaaatcga cccaataggg aacatcttcg ggattcttta
      841 cggaaaaggt gattcctgcg ctgatgccct gattatcgga tcgcacattg acactgtgat
      901 caacgcggga ccgttcgacg gggtgtacgg cgtcctttcc ggactggcag ttgccagggc
      961 gttcagaaat gccggtgtgc gtcctcttcg tccgctcatc gtcgcggcgt tcaccaacga
     1021 ggagggcgta cgttttcagc cggatatgat ggggtcgctc tttttcacgg gcggcatacc
     1081 gatcgacgat gctcttgaaa cgaagggaat cgacggggcg cttctgggtc aagaactgcg
     1141 ccgtattggg tataaggggg aaacggaacc gtcgtctctt ttccctgcgg aatacctcga
     1201 actgcatgtg gaacaggggc cggtactcga cagcaaggaa ttggatatag gagttgtaga
     1261 caatctgcag ggtatttcct ggaagcggat cgagatctcc ggaacggcga accatgccgg
     1321 aacaactccg gtttcgatgc gccgggatgc aggggttgcc gccgctcgaa tcattgtctt
     1381 cctcagggag cttgcgcttt catccgaagg gacgcggacg accgtcgggt cgctgcgttt
     1441 ttaccccgac cttatcaatg tgatcccatc g
//
LOCUS       DAOPKU010000690         1455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_105554_length_1455_cov_3.771429, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000690 DAOPKU010000000
VERSION     DAOPKU010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105554_length_1455_cov_3.771429"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PK849_14970"
     CDS             complement(<1..1005)
                     /locus_tag="PK849_14970"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HPE67483.1"
                     /translation="MLSQKIDAMKDELIAALQESVRIPSVGGDPEEGAPFGAGPRKSL
                     DWTLVFAEKMGFRTKNVENVAGWAEMGEGDEMVAVLGHLDVVPEGKGWTVDPWGGELK
                     DGMIYGRGVLDNKGPVMVGLFAAKAIFDSGIALKRRIRVIFGTNEERGSKCMKRYVEV
                     GEELPAAGFTPDAEYPIIYAEKGILTYTLSLPFAASGEMKLLSLEGGVAANVVMAEVK
                     AEITDPRPECRQRIMDAAAAWKGPEGSSLSAAEDAAQHIVTLEMKGHPAHGSTPEKGI
                     NALACLADFMNGLRLKGEQGNFFARFSKLIGFETNGASLAVAMNDDVSGALTACVGVV
                     K"
     gene            complement(1155..>1455)
                     /locus_tag="PK849_14975"
     CDS             complement(1155..>1455)
                     /locus_tag="PK849_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavoprotein oxidoreductase"
                     /protein_id="HPE67484.1"
                     /translation="FAFQSAVIKTKTRAGYYPGAESMHVKLLGVEGTGRLLGGQIVGG
                     QGSAKRVDIIATALAAGMTVHDLIDLDLGYAPPFSMAWDPVQIAGRELMKKVGGE"
BASE COUNT          270 a          478 c          399 g          308 t
ORIGIN      
        1 cttcaccacg ccgacgcatg cggtgagcgc gcccgagacg tcgtcgttca tcgcaacggc
       61 aagcgaggcg ccgttggttt cgaagcctat aagcttcgag aagcgggcga agaaattccc
      121 ctgctcccct ttcagtctga gaccgttcat gaagtcggcg aggcaggcga gcgcgttgat
      181 gcccttctca ggggtgctcc cgtgggcggg atggcccttc atctcaaggg tcacgatatg
      241 ttgcgcagca tcctccgccg cgctgagcga actcccctcg gggcctttcc aggcggccgc
      301 cgcgtccatg atgcgttgtc tgcactcggg acgagggtcg gttatctccg ccttgacttc
      361 cgccatgacg acgtttgcag ccacgccgcc ttcgagagag agcagcttca tctcacctga
      421 cgctgcgaac ggaagagaga gcgtgtacgt aagaattccc ttctcggcat agatgatggg
      481 gtactctgca tccggggtga atccggccgc aggcagttcc tctccgacct cgacgtagcg
      541 tttcatgcac ttgcttcccc gctcctcgtt cgttccgaag atcacgcgga ttcggcgctt
      601 cagcgcgatg cccgagtcga agatcgcctt cgcggcgaaa aggcccacca tgacgggtcc
      661 cttgttgtcg agaacgcccc tgccgtagat catgccgtcc ttcagttcgc cgccccaggg
      721 atcgactgtc catcccttcc cctcgggaac gacgtcgaga tgcccgagca ctgcgaccat
      781 ctcgtcgccc tcgcccatct ccgcccatcc ggcgacgttc tccacgtttt tcgtcctgaa
      841 ccccatcttc tcggcgaaca caagcgtcca gtcgagcgac ttacggggcc cggcgccgaa
      901 tggagcgcct tcctcgggat cccctcctac gcttgggata cgaacgcttt cctgaagcgc
      961 ggcgatcagt tcgtctttca ttgcatcgat tttctgagag agcacgacaa ttcctcctaa
     1021 aaatgatatc tattatacaa cattcataaa aagagagcgc tcccggaaaa cctcgcacat
     1081 acgatctgcg aagtgcggga gcgtaccctc aactcatcgg gaaagcgctc aggcggattg
     1141 cttttctgaa tgcgctactc ccctccgacc ttcttcatca gttcacgccc ggcaatctgc
     1201 accggatccc atgccatcga aaagggcggc gcatagccga ggtcgagatc gataaggtcg
     1261 tgtaccgtca ttccggccgc aagggccgtt gcgatgatgt cgacccgttt cgcggatccc
     1321 tgaccgccga cgatctggcc cccgagaagg cgtccagttc cctctacacc gagcagcttg
     1381 acgtgcatgc tctccgcgcc ggggtagtat ccggccctgg tcttcgtctt gatgaccgcg
     1441 gactggaagg cgaat
//
LOCUS       DAOPKU010000691         1455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_105558_length_1455_cov_3.695714, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000691 DAOPKU010000000
VERSION     DAOPKU010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105558_length_1455_cov_3.695714"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /gene="mnmG"
                     /locus_tag="PK849_14980"
     CDS             <1..1378
                     /gene="mnmG"
                     /locus_tag="PK849_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPE67485.1"
                     /translation="LGQTPSVELPASLRAAGLEIRRMRTDTTPRLHADTLDLSSLTSQ
                     ASADEPLSFSLWGEGTIHEGYFCHLTRSNRRTHEIIRENLHRSPLSMGWMEGSGPRYC
                     PSIEDKVIKFPEKESHLIFLEPVSRSNKEVYVQNFSTSLPFDVQLAMVRSLPGCEHAH
                     ITRPGYAIEYDYVVPTQLTPWLETKAVSGLFCAGQINGTSGYEEAAAQGLLAGINASL
                     KARGEEPLVLGRSESYIGVLVDDLVTKGTNEPYRMLTSRCEHRLLLRHDNADRRLAPI
                     GRRLGLLDDERWNRLLSGWKRIDDELERLAHAKIPPTDELNRELAALDSAPISEGTKA
                     VELLRRPGVPYSLVEKYSPSPEPPRGGEALSLEVEVKYEGYIERQGRQVARMERMEHV
                     SLPSDMDYGSVPGLLAESRQKLEAVRPYTLGQAGRISGVTPTDIHLLSVWLNALSRRK
                     ERSVAAEE"
BASE COUNT          271 a          455 c          498 g          231 t
ORIGIN      
        1 tctagggcag acgccttccg tggagctgcc tgcgtcgctg cgcgcagccg ggctcgaaat
       61 ccgcaggatg cgcacggaca caacgccgcg cctgcatgcc gacacgctcg acctctcgtc
      121 gctgacctcg caggcgagcg ccgacgagcc gctctccttc tccctctggg gggaggggac
      181 gattcacgag gggtacttct gccacctgac gaggagcaac cgccgcacgc acgagatcat
      241 cagggagaac ctgcaccgct cgcccctttc gatggggtgg atggagggga gcggaccccg
      301 ctactgcccg tccatcgagg acaaggtgat caaatttccg gagaaggaga gccacctgat
      361 ctttctggag ccggtctcgc ggagcaacaa agaggtgtac gtccagaact tttcgacgag
      421 cctgccgttc gacgtccagc ttgcgatggt ccgttcgctt cccgggtgcg agcatgcgca
      481 catcacccgc ccgggctacg ccatcgagta cgactacgtc gtgccgacgc agctcactcc
      541 ctggctggag acaaaggcag tttcgggcct cttctgcgcc gggcagatca acggaacgtc
      601 cgggtacgag gaggccgccg cgcaggggct gctcgcaggg atcaacgcgt cgctgaaggc
      661 gcggggggag gagccgcttg tgctcggccg gtcggagtcg tacatcggcg tgcttgtgga
      721 cgacctggtc acgaagggga cgaacgagcc gtaccggatg ctcaccagca ggtgcgaaca
      781 ccgtctgctg ctgcggcacg acaacgccga ccgcaggctc gctcccatag ggcgcagact
      841 cgggctgctg gacgacgaac gatggaacag gctgctctcc gggtggaagc ggatcgacga
      901 cgagcttgaa cgccttgcgc acgcgaagat tccccctacg gacgagctca acagggagct
      961 ggccgcgctg gactccgccc ccatatcgga gggaacgaaa gcagtggagc tgctccggcg
     1021 ccccggagtg ccctattcgc tcgtcgaaaa gtactccccc tcccctgaac cgccgagagg
     1081 cggggaggcg ctttctcttg aagtcgaggt aaagtacgag gggtatatcg agcggcaggg
     1141 gaggcaggtg gcgcgcatgg agcggatgga gcacgtctcg ctcccttccg acatggacta
     1201 cggcagtgtt cccggtctgc tcgccgagag caggcagaag ctggaagccg tccgtccgta
     1261 cacgctcggc caggcggggc gcatctccgg ggtgacgccc accgatattc atctgctttc
     1321 ggtgtggctg aacgcgctgt cgaggaggaa ggaacgcagt gtggcggcgg aggagtagaa
     1381 gagacccttc aaaggtgggc ggtctgctcc ggggcgtcct cccaagacag gcggaagaga
     1441 ggctcgctct cccgg
//
LOCUS       DAOPKU010000692         1451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_105988_length_1451_cov_3.271490, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000692 DAOPKU010000000
VERSION     DAOPKU010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105988_length_1451_cov_3.271490"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PK849_14985"
     CDS             <1..1393
                     /locus_tag="PK849_14985"
                     /inference="COORDINATES: protein motif:HMM:NF019151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HPE67486.1"
                     /translation="FGQEAYTTYFDAFMRHYLTVRTGDLPRQGEVYEAFKLYARSDGI
                     AQQGVERLVEDIRLYARFFCAMAFDAEKDSDLQRAFHDLRTLKVDVAYPFLLELYARY
                     HEGLLPKKNFLELIRLVESYVFRRAICSIPTNSLNKTFATFAASAKGLEPDRLFESLK
                     AQFLLLPSYRRFPSDEEFRRDFKTRDLYNFRSRSYWLRKIENHERKEHVKTGEYTIEH
                     IMPQNENLSAEWRADLGTEWERVREEWLHTLGNLTLTGYNSEYSDRPFREKRDMDGGF
                     KDSPLKLNKGLGALEKWDEEAIKERAEKLSSMALDIWKAPVLPKETLDTYRSRTARPV
                     SYTIDDHLHLSGKSGNGATKTRALFEILRKEILALDPCVTEEFLKLYVAYKAETNFVD
                     IVPQAKGMRLSLNMPFSDIVDPRSLCKDVKGLGRWGNGDVEVFLSSEDEIPYIMSLIR
                     QSFERQMGNGEEV"
BASE COUNT          349 a          404 c          408 g          290 t
ORIGIN      
        1 cttcggtcag gaggcgtaca ccacctactt cgacgccttc atgcgacatt atctgaccgt
       61 gcgtaccggc gacttgccga gacagggaga agtctacgag gctttcaaac tctacgctcg
      121 ctccgacgga atcgcccagc agggagtcga acgtcttgta gaggacattc gcctttacgc
      181 ccgattcttc tgcgccatgg cgttcgacgc agagaaggat tccgatctgc agagggcgtt
      241 tcacgacctc aggacgctca aggtcgacgt ggcatacccc tttctgctgg agctgtatgc
      301 ccgctatcat gaagggttgc tgccgaaaaa aaacttcctg gaacttatcc ggctggtgga
      361 gtcgtacgtt ttccggcgcg ccatctgttc catccccaca aactcgctca ataagacgtt
      421 cgcaaccttc gccgcctctg cgaaagggct tgagccggat cgccttttcg agagcctcaa
      481 agcgcagttt cttctgctcc cttcttatag gcggttcccg agcgacgagg aattccgccg
      541 cgatttcaag acgcgcgacc tctataactt ccggagccgc agctactggc tgagaaagat
      601 tgagaatcac gaacggaagg agcatgtgaa gacaggagag tacaccatag aacacatcat
      661 gccgcagaac gagaacctct ccgccgagtg gagagccgat cttggtacgg agtgggagag
      721 agttcgggag gaatggctgc ataccttggg aaacctgacg cttacagggt acaactccga
      781 gtacagcgac cgcccgttcc gtgagaaacg cgacatggac ggaggcttca aagacagtcc
      841 gctgaagctc aacaaggggc ttggcgctct cgaaaagtgg gacgaagagg ccataaagga
      901 gagggctgaa aagttgagca gcatggcgct cgacatatgg aaggcgcccg tattgccgaa
      961 ggagactctg gacacctacc gtagcagaac cgcccgtccg gtctcctaca ccatcgacga
     1021 tcacctccat ctttccggca agagcggaaa cggcgccaca aaaacacgcg ccctgttcga
     1081 gatactgcgt aaggagatac tcgccctcga cccctgcgta accgaggagt tcctcaaact
     1141 ctacgtcgcg tacaaggcgg aaaccaattt cgtagacata gtccctcagg cgaaaggtat
     1201 gcgtctttcg ctgaacatgc ccttctctga catcgtcgac ccgcgttcac tctgcaagga
     1261 cgtgaaagga ctgggccgat gggggaacgg cgatgttgag gtcttcctct cctcggagga
     1321 tgagatcccc tacatcatga gcctgatccg ccagtccttc gagcggcaga tggggaacgg
     1381 ggaagaggtg tgaggcggtt tttgatgttt tatcagaaat cagcgaacgc aagaaggaac
     1441 tgcggaattt t
//
LOCUS       DAOPKU010000693         1450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_106070_length_1450_cov_4.037276, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000693 DAOPKU010000000
VERSION     DAOPKU010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106070_length_1450_cov_4.037276"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1199
                     /locus_tag="PK849_14990"
     CDS             192..1199
                     /locus_tag="PK849_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE67487.1"
                     /translation="MDGAEIGTEKGRGAAIEARGLVKRFGEVEAVRGIDFDVYEGELF
                     GFLGPNGAGKTTTINMFTGLARPDEGTLRIGGVDCTANPRAAQRLIGVVPDESNLYPE
                     LTGFENLSFCASLYGMRKAERRKRATELLDMFGLSKAGFRKFGGYSKGMKRKLTIAAG
                     IIHRPRVLFLDEPTTGIDVASARQVRRLIADLHREGTTIFLTTHFIEEAERLCERIAF
                     LVQGRIERIDTVENLLQPLREKHVVHIACAVVLDDVRRALSENFPEASFACMEAGRIR
                     VESEEPIAVGKLVRFLEENEVEVYEARKTTPSLEEVFVRITGIEAEEMRAERAGPGMA
                     R"
BASE COUNT          318 a          382 c          485 g          265 t
ORIGIN      
        1 taaacggaag aaacaaacca cagaagacgt cccgcgaggg gcgtcttttt cttgacattg
       61 aaatggaaag aaaatagtat atccggggcg gttcgtgcgc tgaacccgcc ggccccgctc
      121 ccgtcgagga gtttcttgct ttttcggcgg ctgcgggagt ctttggggga gacctggaga
      181 agaggggtgc aatggacgga gcggagatcg gaacggaaaa gggaagagga gcggcaatcg
      241 aggcgcgcgg gctggtgaaa cgttttgggg aagtggaggc ggttcgcgga atcgatttcg
      301 acgtctacga aggcgaactc ttcggatttc tcggcccgaa cggggccggg aagacgacga
      361 cgatcaacat gtttacaggg ctggcacgcc ccgacgaggg gactctccgc atcggcggcg
      421 tggactgcac cgcaaatccg cgggcggcgc agcggctgat cggcgttgta ccggacgaga
      481 gcaatcttta cccggagctg acaggattcg agaacctctc gttctgcgct tcgctctacg
      541 gaatgcgcaa agccgaacgg aggaaacggg ctacggagct tctcgacatg ttcggactga
      601 gcaaagccgg attcaggaag ttcggcggct actccaaggg gatgaagcgg aagctgacca
      661 tagccgccgg gatcatccac cgtccgcgcg tcctcttcct ggacgagcca accacaggaa
      721 tcgacgtcgc gagcgcgagg caggtacggc gcctcatcgc cgatcttcat cgggagggga
      781 cgaccatctt tctgacgacg cacttcattg aggaggcgga gcgcctgtgc gaaagaatcg
      841 cctttcttgt gcaggggagg atcgagcgga tcgacacggt cgagaatctg ttgcagccgc
      901 tccgggagaa gcacgtcgtg cacatagcat gcgcggtcgt gctggacgat gtgcgtcgag
      961 ccctctccga aaacttcccg gaggcgtcgt ttgcctgcat ggaggctggg agaatccgtg
     1021 tcgaaagcga ggagccgata gcggtcggca agcttgtccg cttccttgaa gagaatgagg
     1081 tcgaggtgta cgaggcgagg aaaacaactc cctccctgga ggaggtcttt gttcgcatca
     1141 ccggtatcga ggccgaggag atgcgcgcgg agagagccgg accgggaatg gcgcgataaa
     1201 cgaccgggag gcaaaaatga ccatgaaaaa agacgtccgc gaacaggacg tctttttatc
     1261 gtctgaagat gttgatgaag cagattacgc cgacatcgcg aactcgcccc acctggagaa
     1321 gtcctgtgcg cctttttcgc cggagtaggc tttttcagcc tcttgctttg ccatctcggc
     1381 gcgtgcgcgc atctgggccg cagaagctgc ggccgcgacc tgtatatcct gcggagatgg
     1441 agaacctgcc
//
LOCUS       DAOPKU010000694         1443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_106904_length_1443_cov_2.532421, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000694 DAOPKU010000000
VERSION     DAOPKU010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106904_length_1443_cov_2.532421"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK849_14995"
     CDS             complement(<1..444)
                     /locus_tag="PK849_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPE67488.1"
                     /translation="MTNMDMSQYMGAFLDEAGDNLKHLDDLTLVIEKDPGNSEAIAEI
                     FRSAHTLKGMSATMGFDRMASLTHAMEDMLDAVRKGEYSLSAGDIDLLFRSLDTLQSM
                     VDAIRSVGSDASVDTDALVKSIRAAGVNRGAEPPAPEEKALNDELS"
     gene            complement(484..>1443)
                     /locus_tag="PK849_15000"
     CDS             complement(484..>1443)
                     /locus_tag="PK849_15000"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HPE67489.1"
                     /translation="DALRKIEELSPAVVSLDVEMPKKDGLATLEEIMKRRPLPVIMVS
                     SLTQEGAQITLRALSAGAVDFVAKPSGHVSLNMRDVSADLITKVLAASTAKIVVPRER
                     REPRAQPVAPTAARPMESFASRWRKGRPEMVAIASSTGGPRALQQVLSQIPGDFPVPI
                     VIVQHMPKDFTLSFAKRLDSLSELDVVEGAEGTELRPGLAVVAPGGYHMVVRKKSPGK
                     FACALSDAPPLLSVKPSANILFLSVADEIGGSTVGVILTGMGRDGADGAAALKMKGAR
                     IIAESQETCVVYGMPKAAVEQGVVDELLPLYSIPEAMIRSVRE"
BASE COUNT          299 a          450 c          383 g          311 t
ORIGIN      
        1 ggagagctcg tcgttcaagg ctttttcttc cggcgcaggc ggttcagctc ctctgttaac
       61 ccccgcggct cgtatagact ttacgagagc gtcggtatcc accgaagcgt cgcttccaac
      121 cgaccgtatg gcgtcgacca ttgactgcag cgtatcaagc gaacggaaga gcaggtcgat
      181 atcccccgcg cttaaagaat attccccttt tcggacggcg tcgagcatgt cctccatggc
      241 gtgggtgagg gaagccatcc tgtcgaaccc catcgtcgcc gacatccctt tgagggtgtg
      301 cgccgaccgg aagatctcgg cgatagcctc cgaattcccc gggtcttttt cgatcacgag
      361 cgtaagatcg tcgaggtgct tcagattgtc ccccgcttcg tcgaggaaag ctcccatata
      421 ctggctcata tccatattcg tcatggcaga tcatctccag ccttgtaaac tcgtttcatg
      481 tcgttattct ctcacgcttc ggatcattgc ctcgggtatt gagtacaggg gaagaagctc
      541 gtcaacaacg ccctgttcca ccgcagcctt cggcattccg tacacaacgc acgtctcctg
      601 agactctgcg atgatgcgcg ctcctttcat cttgagcgcg gctgcgccgt cggctccgtc
      661 ccgtcccatt ccggtgagaa tcaccccgac cgtactccct ccgatctcgt cggcgacgct
      721 caaaaacagg atgttcgccg acggcttgac ggaaagaaga gggggcgcat cggaaagagc
      781 gcaagcgaat tttcccggcg atttcttccg cactaccata tgataccctc caggagccac
      841 tacggctagc cccggacgta attccgttcc ttcggccccc tcgacgacat cgagttcaga
      901 cagagaatcc agtcgttttg cgaaagagag ggtaaaatcc ttcggcatgt gctgcacaat
      961 gacaatggga accgggaaat ccccgggtat ctgagaaagc acctgctgca acgctctggg
     1021 accgccggtt gatgaggcta tggcgaccat ctcggggcgt cctttcctcc agcgcgaggc
     1081 aaacgactcc atcgggcgcg ccgcggtagg cgcaacgggc tgcgcacgcg gttcccgccg
     1141 ttcacgcgga acgacgattt tcgccgtgct tgccgccagt acttttgtaa taagatctgc
     1201 ggagacgtcc cgcatgttca gcgaaacatg ccccgaaggc ttggcgacaa aatccaccgc
     1261 cccggccgaa agcgccctga gcgtaatctg cgcgccttcc tgcgtcaggc tgctcaccat
     1321 gatcacagga agaggacggc gcttcatgat ttcctcaaga gtagccaaac cgtctttttt
     1381 gggcatctcg acatcaagcg aaacaaccgc cggtgaaagc tcttcaatct tgcgaagcgc
     1441 gtc
//
LOCUS       DAOPKU010000695         1439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_107372_length_1439_cov_2.058526, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000695 DAOPKU010000000
VERSION     DAOPKU010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107372_length_1439_cov_2.058526"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PK849_15005"
     CDS             complement(<1..384)
                     /locus_tag="PK849_15005"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPE67490.1"
                     /translation="MNEVLRAIFERRSIRKYSAEPVKREDIELVLQAGLYAPTGGDAE
                     PWHFGVQTDREMIEDLDERARAAMRESGIERISVMGANPKYRVFFGAPVVIMIYGERT
                     LRKSGTHLSALADCSAAIQNMQLAAF"
     gene            complement(488..1144)
                     /locus_tag="PK849_15010"
     CDS             complement(488..1144)
                     /locus_tag="PK849_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67491.1"
                     /translation="MLIPEERIDVIIEFLDVDGAGQVYITAEGIRRLQALLTTVEIPE
                     HGATLSLPVFRALLLGGASSTGSGSGLGESALVFFGVPEAFIGKNVENLHVVKMLSTE
                     SAEVLTHVFDLDDLRDGTCAVVEIEQTAEGEKLKRVLEAKDSVTPMSRIAMALQDGGE
                     YDLDAAENGGVTDPAFILEATLKEQVSSGGSGCSLGGEYAHLSAIALLLPALLLFLRK
                     "
     gene            1137..>1439
                     /locus_tag="PK849_15015"
     CDS             1137..>1439
                     /locus_tag="PK849_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67492.1"
                     /translation="MSNLRSALRRASLFWSAGGAGGSSTVRGTGIAGYTPSGRSHPIT
                     GGGTGWRGMGVGVGVGVGVGVGVGVGHGVGSGVGSGVGMGVGMGVGVGVGVGVGVGV"
BASE COUNT          275 a          444 c          419 g          301 t
ORIGIN      
        1 aaaggccgcg agctgcatgt tctggatggc cgcgctgcaa tcggccagcg ccgagaggtg
       61 cgtcccgctt ttgcggagcg ttcgctcccc gtagatcatg atcaccaccg gagcgccgaa
      121 gaagaccctg tacttcgggt tggcgcccat gaccgagatc ctctcgatgc cggactcccg
      181 catggcggcg cgggcgcgtt cgtcgagatc ctcgatcatc tctctgtccg tctgcacgcc
      241 gaagtgccac ggctcggcgt cccctccggt cggcgcatag agccccgcct gcagcacgag
      301 ttcgatatcc tcccgcttca caggttcggc cgaatattta cggatgctgc gccgttcgaa
      361 tatggcgcgc agaacttcgt tcatttcact tgcctccttc tgaaaaaagc ccgggcgatc
      421 ctcttgatgc aatcacccgg acttctttta acgcgagtgt acgcacatat ccgcgctgaa
      481 aggatagtta tttcctcaaa aagagcagca acgccggaag gagcagggcg atcgcggaaa
      541 gatgcgcata ctccccgcct agcgaacagc cgctcccacc gctggaaact tgctctttca
      601 aggtcgcctc caggataaat gcggggtccg tgacacctcc attctccgcc gcgtcaagat
      661 cgtattctcc gccatcctga agcgccatgg caatccggga catgggggtc acactatcct
      721 tcgcctccag cacgcgcttc agcttctccc cttcggcagt ttgttcgatc tccaccaccg
      781 cgcacgttcc gtcgcggaga tcatcaagat cgaacacgtg cgtcaacacc tctgcgcttt
      841 ccgtcgacag catcttgaca acgtgcagat tttccacatt tttccctata aacgcctcag
      901 gtacaccgaa gaataccagt gctgattctc caagccccga tcccgagccc gttgaagaag
      961 cgccgccaag tagaagcgcg cggaacacgg gcagcgaaag agtcgctcca tgctcaggta
     1021 tctccaccgt agtcaagagc gcttgcagac ggcgtatccc ttcggcggtg atgtacactt
     1081 gccctgcgcc gtccacatcc aagaactcaa tgatgacatc gatgcgttcc tccggtatga
     1141 gcaacctgcg aagcgcattg cgaagggctt cccttttctg gtcggccggc ggagcgggtg
     1201 gcagctcgac tgtcagggga acaggcatcg ccggatacac tccatccgga agaagccatc
     1261 cgataaccgg cggaggaacg ggttggagag gtatgggcgt tggcgttggc gttggcgtcg
     1321 gcgtgggcgt cggagttggc gttggacatg gcgtcggatc tggcgtagga tctggcgtcg
     1381 gtatgggcgt cggtatgggt gtcggcgttg gcgttggcgt cggggttggc gttggcgtc
//
LOCUS       DAOPKU010000696         1438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_107410_length_1438_cov_4.105568, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000696 DAOPKU010000000
VERSION     DAOPKU010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107410_length_1438_cov_4.105568"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PK849_15020"
     CDS             <1..373
                     /locus_tag="PK849_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPE67493.1"
                     /translation="PPAELRKIADAVVKGLNETVAFIRPGVTAEAVEEKWRESIAGTG
                     VHKPSRVGYSFGLNYPPDWGEQTLSLRPADTTILQPGMCIHFMPGIWLKDFGFECSEP
                     LVVTGSGCELFLNFERKLFVK"
     gene            470..1144
                     /locus_tag="PK849_15025"
     CDS             470..1144
                     /locus_tag="PK849_15025"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE67494.1"
                     /translation="MENVTFERDTLSERVADFIRRQILYTDVFKDGDHILETDIADHL
                     QVSRATVREALKDLETQGIVEIIPRKGTYVAAFDHKDMIEILELRCMCEAYIFETVVN
                     GDMLDARDFAVLESIIDEMAQVSRSAEESELAKSIAFTTKDLEFHGYIWKKSGRRWFC
                     KVLSDNYYRLRLAMMQDMILERDMEKSATMHYDILNALREKDLDTARNYLRRHILSLH
                     GAETVA"
     gene            1232..1423
                     /locus_tag="PK849_15030"
     CDS             1232..1423
                     /locus_tag="PK849_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67495.1"
                     /translation="MFKAKHLREIQAFMEKTGIPGRDGWELPASKKSFPDGANYRIEI
                     AGVERYSNMVAMVDEAKKR"
BASE COUNT          329 a          421 c          456 g          232 t
ORIGIN      
        1 gccgcccgcg gagcttcgaa agatcgcgga cgccgtcgtc aagggcctga acgagacggt
       61 cgccttcatc cgtccgggcg tcacggccga ggcggtggag gagaagtgga gggagtccat
      121 cgccggaacg ggcgtgcaca aaccgtcgcg cgtcgggtac tccttcggcc tgaactaccc
      181 gcccgactgg ggcgagcaga cgctcagcct ccgcccggcg gatacgacca tcctccagcc
      241 ggggatgtgc atccacttca tgccgggcat ctggctgaag gatttcggct tcgagtgcag
      301 cgagccgctc gtcgtcaccg ggagcgggtg cgagctcttc ctgaacttcg agcgaaaact
      361 gtttgtcaaa tgaccgaaat gatagaataa gagagcggac cacatacaat agaccagaga
      421 gaacgaacgg ggagcggtga tctcctcccc gggaaacgga gagcgcgcca tggaaaacgt
      481 caccttcgaa agggatactt tgagcgagcg ggtcgccgac ttcatccggc ggcagatact
      541 gtacacggac gtcttcaagg acggcgacca catccttgag accgatatcg ccgatcacct
      601 gcaggtgagc agggccacgg tccgcgaagc cctcaaggac ctggagacgc aggggatcgt
      661 cgagatcatc ccccgcaagg ggacgtacgt cgcggccttc gaccacaagg acatgatcga
      721 gatcctcgaa ctccgctgca tgtgcgaggc gtacatcttc gagacggtcg tgaacgggga
      781 catgctcgac gcgcgcgact tcgccgtcct cgaatcgatc atcgacgaga tggcgcaggt
      841 ctcccgctcg gcggaggaga gcgagctcgc caagtcgatc gccttcacca cgaaggacct
      901 tgagttccac ggctacatct ggaagaagtc cggccggcgc tggttctgca aggtgctctc
      961 ggacaactac tacaggctgc ggctggccat gatgcaggac atgatcctcg aacgggacat
     1021 ggagaagtcg gccacgatgc actacgatat tctgaacgcg ctcagggaga aggacctgga
     1081 cacggccaga aactaccttc gcagacacat tctctcgctc cacggcgccg aaacggtcgc
     1141 ctgacgacga gcctgccccg tgcgggcttt ttttaccggc ggagagtgtg tcgacaaaaa
     1201 aatgacgaca ttaaaaggag gcgcttcagg gatgttcaag gcgaaacacc tcagggagat
     1261 tcaggcgttc atggagaaga cggggattcc gggaagggac ggatgggagc tgcccgcctc
     1321 gaagaagagc ttccccgacg gggccaacta ccgtatcgag atcgccggag tggagcgcta
     1381 ctccaacatg gtcgccatgg tggacgaggc gaagaagcgc taaattccca tccacagg
//
LOCUS       DAOPKU010000697         1429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_108440_length_1429_cov_5.567686, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000697 DAOPKU010000000
VERSION     DAOPKU010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108440_length_1429_cov_5.567686"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /gene="dctP"
                     /locus_tag="PK849_15035"
     CDS             <1..462
                     /gene="dctP"
                     /locus_tag="PK849_15035"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE67496.1"
                     /translation="QLMMGLVSALGAVPTPMPFGEVYSALQTGVIDGAENNWPSYFST
                     SHYEVAKYFTLDGHTRVPEILIASKISMDKLSKEDQEIIKQAAKDSMPYQIKLWKEFE
                     KVSEEKVRAAGSIITELTPEAVVEFQNAMQPMYDALSPELQEVVKKIRDVQ"
     gene            524..1045
                     /locus_tag="PK849_15040"
     CDS             524..1045
                     /locus_tag="PK849_15040"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE67497.1"
                     /translation="MNAVTSFFRLLHTLLVILAKTLLAAMVLLIAANVFMRYVLNSGI
                     TWSEEVALVFVVWFTFIALALGVKQRLHISLCLLPEKISPKIDFLLSKLTDIVILFMG
                     YIMIHYGWILVQFTSRSILPATGVPASVLYFPLVLSGVLVLYEGGMNLLGLDKGDANM
                     DDKFSGRRCASDV"
     gene            1038..>1429
                     /locus_tag="PK849_15045"
     CDS             1038..>1429
                     /locus_tag="PK849_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE67498.1"
                     /translation="MSDPTVATWILLGSFALFLVLKAPITFSLAVSSVLTAMYLKIPL
                     MAIVQQMVQGVNSFSLLAIPFFIIAGEMMGEGGISRRLIAFSNLLVGRVRGGLAQVNV
                     LASMFFGGISGSAVADVSSIGTILIPMM"
BASE COUNT          275 a          457 c          367 g          330 t
ORIGIN      
        1 cagctcatga tgggtctcgt atcggctctc ggcgccgtcc ccacccccat gcccttcggc
       61 gaagtgtaca gcgcgcttca gaccggcgtc atcgacggcg cggagaacaa ctggcccagc
      121 tacttctcca ccagccacta cgaggttgcg aagtacttca ctctcgacgg gcacacccgc
      181 gttcccgaga tcctgatcgc gagcaagatc agcatggaca agctctccaa agaagaccag
      241 gagatcatca aacaggccgc gaaggactcc atgccctatc agatcaaact ttggaaagag
      301 ttcgagaagg tctccgaaga aaaagtccgc gccgcgggtt cgatcatcac cgagctgaca
      361 cccgaggcag ttgtcgagtt ccagaacgcc atgcagccga tgtacgacgc cctcagcccc
      421 gaactgcagg aagtagtcaa gaaaattcgc gacgtacagt aacggctcga aaagggaagg
      481 ctcctgaggg agtcttccct tttttcagga aggaggtctg cccgtgaacg ctgtcacatc
      541 gttcttccgg ctgctgcaca cgctcctcgt cattctcgcg aagacgctgc tcgcagcaat
      601 ggtgcttctc atcgccgcca acgtcttcat gcgctatgtc ctcaactccg gcatcacctg
      661 gtccgaggag gtcgcgctcg tcttcgtcgt ctggttcacc ttcatcgctc ttgcgctcgg
      721 cgtcaaacaa cggcttcata tcagcctctg ccttcttccg gagaagatct ccccgaagat
      781 cgactttctc ctctccaaac tgaccgatat cgtcattctc ttcatggggt atatcatgat
      841 ccactacgga tggatcctcg tccagttcac cagccgctct atccttccgg cgacaggggt
      901 tcccgcctcc gtactctatt ttccgctcgt actctcgggc gtgctcgtcc tctacgaagg
      961 cggaatgaat ctgctcggac tcgacaaggg cgacgcaaac atggacgata aattctccgg
     1021 caggaggtgc gcctccgatg tctgatccga ccgtcgctac gtggatcctc ctcggcagct
     1081 ttgccctctt ccttgtcctc aaggcgccga tcaccttttc cctggcggtt tcctccgtgc
     1141 ttaccgcgat gtacctgaag attcccctca tggctatcgt ccagcagatg gtgcagggag
     1201 tgaactcttt ctcgctgctc gcgattccat ttttcatcat cgccggcgag atgatgggcg
     1261 agggtggtat ttcacgtcgg ctcatcgctt tctcgaacct gctcgtaggg cgcgttcggg
     1321 gcgggctcgc ccaggtcaac gtgctggcga gtatgttctt cggcggcatc tccggttccg
     1381 cagtggcgga cgtctcctct atcggcacca tccttattcc gatgatgaa
//
LOCUS       DAOPKU010000698         1427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_108741_length_1427_cov_2.979592, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000698 DAOPKU010000000
VERSION     DAOPKU010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108741_length_1427_cov_2.979592"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..556)
                     /locus_tag="PK849_15050"
     CDS             complement(161..556)
                     /locus_tag="PK849_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67499.1"
                     /translation="MKKSFRYIFFLFSYTFTFLFFFPGASSANYVLIPAQAPAIITTP
                     DYVDSFQAPSPSALSGQIFRSKEGYLFSFDASGRRVYHMHASYVLSAHGSSPVPVHII
                     AAPMIAVHPGAVLTPSPYTYLPQQYLLVP"
     gene            complement(620..>1427)
                     /locus_tag="PK849_15055"
     CDS             complement(620..>1427)
                     /locus_tag="PK849_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67500.1"
                     /translation="NPKGGWFTMRRRILIVLSLTVLLSLLPSTTTATTYVVEPVLVAQ
                     PVYSGMSFYVYRPYNMPSGWFVTFDGYPITRTPAGVWVYGSYNGSLLAPTSFVVGSIS
                     PQSLPIVQYVPSARVSSTQSLPVTPLPPLPAPIQPDPGAYILAEAVTVAPTYVPEWTT
                     QSSFTSIGTWNKLVDRMGILEKPRVPIAWKGERPEALFVWTGKSWYQIQARSGVSEKP
                     SEMIKDHLYALTRMVKNNAYHWKDADTAILANQAAVWGYLWMGRIAPSNL"
BASE COUNT          398 a          299 c          383 g          347 t
ORIGIN      
        1 tcacacttct ttaacacctc ggttcaagcg acttctatta tgacacaggg gggtatacga
       61 caacgacgct ctccgggact ttcatgacga agagccgccc agtgaggatt tcctcaaggg
      121 cggctcttcg tcatgatgat gtcagagaga aaaacgagat ttacggtaca agcagatatt
      181 gttgcggaag atatgtatag ggggatggag tgagcacagc gcccggatgt actgcaatca
      241 tcggggcagc aataatatgt accggaaccg ggctgctgcc atgagcggaa agaacatacg
      301 aagcgtgcat gtgataaacg cggcgtcctg aggcgtcaaa tgagaaaaga tatccttcct
      361 ttgagcggaa aatttgtcca gatagagcag agggggacgg agcttgaaaa gaatcgacat
      421 agtcaggagt tgtaataatt gccggtgctt gcgcaggaat gaggacataa ttcgcagaag
      481 acgcacccgg aaagaaaaaa agaaacgtga atgtgtaaga gaacaagaaa aaaatatacc
      541 tgaaagattt tttcatctga ttgccccctt gttttatctc aagctaacga gaagtgtctt
      601 ttgaacgaag aacgattatc tataggtttg aaggagcgat tcttcccatc caaagatatc
      661 cccagactgc ggcctgattg gcaagaattg ccgtatctgc gtctttccag tggtatgcgt
      721 tgttcttcac cattcgagta agcgcataaa gatgatcttt tatcatttct gatggttttt
      781 ctgaaacgcc ggagcgggct tgaatctggt accagctctt tcccgtccag acgaaaaggg
      841 cttcaggacg ttctcccttc catgcgatcg gaactcttgg tttctcaaga attcccattc
      901 tatcgaccag tttattccaa gtgcctattg aagtaaaact tgattgtgtt gtccactccg
      961 ggacatatgt cggagcgact gtcacggctt cagccaaaat ataagccccc ggatcaggtt
     1021 ggattggggc ggggagcggt ggaagcggcg taaccggtag gctctgtgtg ctggaaacac
     1081 gtgctgaagg tacatactgg acaataggaa gtgattgggg agagatagaa ccgactacaa
     1141 acgacgttgg agcaagaagg gatccgttat atgaaccata aacccagact cccgcagggg
     1201 tgcgtgttat tggatacccg tcgaacgtga caaaccagcc ggagggcata ttatatggcc
     1261 ggtagacata aaaggacatt cctgaataca cgggttgcgc cacgagaacc ggttcgacaa
     1321 cgtatgttgt cgctgttgta gttgatggta ggagagagag caggactgtt aagctgagta
     1381 cgatgagtat tcttcttctc atggtaaacc atcctccttt gggattt
//
LOCUS       DAOPKU010000699         1425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_108945_length_1425_cov_3.287591, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000699 DAOPKU010000000
VERSION     DAOPKU010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108945_length_1425_cov_3.287591"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PK849_15060"
     CDS             complement(<1..1272)
                     /locus_tag="PK849_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67501.1"
                     /translation="MAGEIALDEERAELVGAEHYKIGDPTQGGTEGTAELIRGLQQSI
                     RDYSEREWTARRGKREYPSYYDAFIQSSNDKEKVSALMPAISDPLRLAETSIKDMMDT
                     LTREVLVNQFMLAEDAAQNPFVIAIPREGVKDTDPANRGRDIRSMLREDTLRKQLRML
                     ERKAGRAGRGDTLHEKLRSLVSGNERAVNLDNYELVKSDYESIQAFYVLKKEAGYDSR
                     ARYWFQHVVGRPAQWNVEIGEEFEHDVLKTLSKVNMFMKHMAVAWSLFFATAGVESTV
                     AATPLNRNMANVFQKQFWENWLSVARLGRRVRRGDLEIAMDLYKYHKAGIMISERNIS
                     DGSSSVVDEVISKVSRSLGERFGGEDDAKRAMYEKRSRTALRTLTGRKLSEWLMGDFF
                     GGLKLWSTQFLIDEFHKKNPHLSEESVLEIVA"
     gene            1216..>1425
                     /locus_tag="PK849_15065"
     CDS             1216..>1425
                     /locus_tag="PK849_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPE67502.1"
                     /translation="MLRPDQLRPLLIERNFTRHAEGSVLIRMGVTHVLCNASVEENVP
                     PFLRGKGQGWVTAEYGMLPRSTHTRS"
BASE COUNT          240 a          478 c          386 g          321 t
ORIGIN      
        1 ggcgacgatc tctagcacgg actcttccga gaggtgcggg ttcttcttgt ggaactcgtc
       61 tataagaaat tgcgtcgacc acaatttcag tccgccgaag aagtccccca tcaaccactc
      121 ggacagtttg cgtccggtca gcgtgcgcag cgcggtgcgc gaccgcttct cgtacatggc
      181 ccgcttggcg tcgtcttctc ctccgaaccg ctctccaagc gagcgagaca ccttggatat
      241 cacttcgtcc accaccgagc tggagccgtc agagatgttg cgctccgaga tcatgatgcc
      301 ggccttgtgg tacttgtaca ggtccatggc gatctcgagg tctccgcgcc gcacccttcg
      361 gccgagcctt gctaccgaca accagttttc ccagaactgc ttctgaaata cgttggccat
      421 attcctattc agaggggtgg cggccacggt cgactcgacg ccggcggtcg cgaagaacag
      481 gctccacgca acggccatgt gcttcatgaa catgttgacc ttcgacagcg tcttcaacac
      541 gtcgtgctca aactcctccc cgatctccac gttccattgc gccggcctcc caacgacgtg
      601 ctggaaccaa taccgcgccc ggctgtcgta ccccgcctct ttcttcaaca cgtagaaagc
      661 ctggatagat tcgtaatcgc tcttgaccag ctcgtagttg tcgaggttta cggcgcgctc
      721 gttcccgctg accaggctgc ggagcttctc gtgcagcgtg tccccgcgcc cggcacgccc
      781 cgccttgcgc tccagcatcc gaagctgttt tcgaagcgtg tcctcccgca gcatgctgcg
      841 gatgtctctt ccgcgattcg ccgggtcggt atctttgacc ccctctcttg ggatcgcgat
      901 gacgaacgga ttctgcgcgg cgtcctcggc cagcatgaat tgattcacga ggacttccct
      961 cgtgagcgtg tccatcatgt ctttgatgga cgtctccgcg agtcgcagcg ggtccgatat
     1021 cgcgggcatc agcgcggaca ccttttcctt gtcatttgag gactgtatga aagcgtcgta
     1081 gtatgacggg tactcccgct ttcctcgacg agccgtccac tcccgttcgg agtagtctcg
     1141 tatggactgc tgcagccccc ggataagttc tgcagttcct tcggtgccgc cctgcgtcgg
     1201 gtccccgatt ttgtagtgct ccgccccgac cagctccgcc cgctcctcat cgagcgcaat
     1261 ttcacccgcc acgccgaagg ctcggtgctg atccgcatgg gtgtcaccca tgtgctatgc
     1321 aatgccagcg tcgaggaaaa cgtgccgccc ttcctgcgcg gcaagggcca gggctgggtg
     1381 accgccgaat acggcatgct gccacgctcg acccataccc gttcg
//
LOCUS       DAOPKU010000700         1424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_109055_length_1424_cov_4.297297, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000700 DAOPKU010000000
VERSION     DAOPKU010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109055_length_1424_cov_4.297297"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PK849_15070"
     CDS             complement(<1..733)
                     /locus_tag="PK849_15070"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPE67503.1"
                     /translation="MAEITVFGGGTWGTALAVSAAKSGHSVMLWCRRAEQARAINATS
                     HNPDYLQAIELPEAISSTSDPEEAALFSQHWILALPTQTVRGFLPALAPFCGKETEVC
                     NVAKGIEIGTGARISAIVSETLPEVRYSVLSGPSFAGEVAQGLPTALTAASRHETSAL
                     LWQSALNTQRLRIYTSSDVIGTETGGAVKNIMAIASGMASALGLGENARAALVTRGLA
                     EIMRLGEALGAHPLTLAGLAGMGDLV"
     gene            complement(758..>1424)
                     /locus_tag="PK849_15075"
     CDS             complement(758..>1424)
                     /locus_tag="PK849_15075"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE67504.1"
                     /translation="LMLFAFVLFLPDARNAGGSPSLSVYKRLFRDKDIWKINMATFCS
                     LYGFWAAATWGPTFLQAERGFSLSGSGLYTGLIALSALPGGVLWGRLSDRFGRRKIAA
                     LVLPASGVALFLLTRVQGAPLIVSTLLLFGIFSNTAFSPVAVAWIGDIVSKRHPGSMS
                     AAVGFFNATIMSSAVIAPLVSGFLRDMTGSLVPAILTGSLLMFFGTVMLLLTPDAARA
                     GAE"
BASE COUNT          282 a          439 c          473 g          230 t
ORIGIN      
        1 ggacgagatc ccccattccc gcgagcccag cgagcgtcag cggatgggct ccgagcgctt
       61 cgccgaggcg catgatttcc gcaagtccgc gggttaccag cgctgcgcgc gcgttttctc
      121 ccaaaccaag ggcggaagcc attccggagg cgatcgccat gatatttttc accgcgcccc
      181 ccgtttcggt accgatgacg tcggacgacg tgtagatgcg cagtctctgg gtattcagcg
      241 cagattgcca gaggagcgcg gacgtctcgt gcctggaggc ggcagtcagt gcggtcggaa
      301 ggccttgcgc cacctccccg gcgaaactcg gtcccgaaag gacggagtag cggacttccg
      361 gaagcgtctc cgaaacgata gcgctgattc ttgctcccgt gccgatctcg atcccctttg
      421 ccacgttgca gacttcggtc tctttcccgc agaagggggc gagcgccgga aggaaacccc
      481 gcaccgtttg cgtcggcagc gcgaggatcc agtgctggga gaagagcgcc gcctcctctg
      541 gatccgaggt ggacgagatc gcctcgggaa gctcaattgc ctggaggtag tccgggttat
      601 gcgaggtcgc gttgatcgct cgcgcctgtt cggcgcgacg gcaccagagc atgacggagt
      661 gcccgctctt ggcggccgag actgcgagcg ccgtacccca ggttcctccg ccgaatactg
      721 tgatttcagc catgacggga attgctcctt tcgtgtatta ctccgcgccc gcccgggcgg
      781 cgtcgggagt cagcagcagc attacggtac cgaagaacat cagcagggag cccgtgagga
      841 tggcgggtac cagggagccc gtcatgtcgc gcagaaagcc ggagacgaga ggcgcaatca
      901 ccgccgagga catgatcgtc gcgttgaaaa aaccgaccgc cgcgctcatc gagccagggt
      961 ggcgtttgct cacgatgtcg cctatccatg cgactgcaac gggcgaaaac gccgtgttgc
     1021 tgaagatgcc gaagagcagc agcgtggaaa cgatgagcgg cgctccctga acgcgggtga
     1081 gaaggaagag cgccacgccg ctcgccggca gcacgagcgc ggcgatcttc ctgcggccga
     1141 agcggtcgga gagcctgccc cagagcacgc ctcccggaag cgcggagagc gcgatgagcc
     1201 cggtgtacag cccggagcct gagagcgaaa acccccgctc cgcctgaagg aacgtcggtc
     1261 cccatgtcgc ggcggcccag aagccgtaga gcgagcagaa cgtcgccatg ttgatcttcc
     1321 agatgtcctt gtctcgaaag agacgcttgt agaccgaaag cgacggggaa cctccggcgt
     1381 ttcgcgcgtc ggggagaaaa aggacgaatg cgaagagcat aagc
//
LOCUS       DAOPKU010000701         1414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_110299_length_1414_cov_2.922001, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000701 DAOPKU010000000
VERSION     DAOPKU010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110299_length_1414_cov_2.922001"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="PK849_15080"
     CDS             <1..1395
                     /locus_tag="PK849_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HPE67505.1"
                     /translation="ASSGLRYVLWRKGDDGRPVVIHSTVNGDAERILSEGVVTGLDIL
                     NAEWFLSALPEGGWGNLPLVLLLSFGGLALSVQLALVARLVAGIRDSEEKYRLLAENV
                     KDVIWMVSLPDFKFLYISPSVEELSGYSVEETMNMRLDEYLEPESLDHVKKLYGESLC
                     RFRKGEIPEEGRVMEVRHRRKDGSSVWLGVRSSYILNDDGEPVGILGISRDMTEIKHV
                     QQELERANATKNTFLAIIAHDLRGPVGALGQTLELLEQNADAMDEEDLEEFLSLASGD
                     ARRVFSLLENLLTWSRSQLEQIVFRPVVWGVRIIVEESVELLASRASEKGIVLSNDAP
                     PMMTARLDPGMMGVVLRNLVGNALKFTSPGGSVSVSARRTDEGVEFAVRDTGRGIPPE
                     IAEKLFRIDVGHGSTEGTAGERGSGLGLILCKEFVEKHGGRIWFESSPGEGTVFFFSL
                     PDRLNGDPAEERGV"
BASE COUNT          285 a          356 c          473 g          300 t
ORIGIN      
        1 gcctcaagcg gtctgcgcta tgttctctgg aggaagggcg acgacggacg tcccgtggtc
       61 attcattcaa ccgtgaacgg agatgcggag cggatccttt ccgaaggtgt cgttaccggg
      121 ctcgatattc tgaacgccga gtggttcctg agcgcccttc ccgagggcgg gtggggaaat
      181 ttaccgctgg ttttgctgct ctcgtttgga ggactcgcgc tgagcgtgca gcttgcgctt
      241 gtggccaggc ttgtcgccgg gatacgcgac agcgaggaga agtaccgtct tctggccgag
      301 aacgtcaaag atgtgatatg gatggtatcg ttgcccgact tcaagtttct ctatatcagc
      361 ccgtcggttg aagaactcag cgggtattcc gtcgaagaga cgatgaatat gcgcctcgac
      421 gagtatctcg agccggaatc gctcgatcac gtgaaaaaac tgtacggcga aagtttgtgc
      481 cgcttccgca agggagaaat ccccgaagaa ggaagagtca tggaggttcg tcatcgacgg
      541 aaagacggct cgtccgtctg gctgggagtc cggagcagct atatcctgaa cgacgatggc
      601 gaacccgtcg gtattcttgg aatttcgcgc gacatgacgg agatcaagca tgtgcagcaa
      661 gagcttgaac gcgccaatgc gacaaaaaac acgtttctcg ccatcatcgc gcacgatctg
      721 cgagggcctg tcggcgcgtt ggggcaaacg ctcgaactgc tggagcagaa cgcggacgcc
      781 atggacgaag aagatttgga ggaatttctt tctcttgcaa gcggcgacgc gcgaagggtt
      841 ttttcgctgc ttgagaatct gttgacctgg tccaggtcgc agctcgagca gatcgtattt
      901 cggcctgtcg tgtggggtgt gaggataatc gtggaggaga gcgtcgagtt gcttgcctcc
      961 cgcgcatccg agaagggaat cgttttgtcg aacgacgctc cgcctatgat gaccgcccgg
     1021 ctggatccgg ggatgatggg cgtcgtgctg cgaaatcttg tcggaaacgc gctgaagttc
     1081 acttccccgg gcggcagtgt ttccgtctcc gcgcggcgga cggacgaggg cgtcgagttt
     1141 gccgtccgcg ataccgggcg cggcatacct ccggaaattg cggagaagct cttccgtatc
     1201 gacgtcggtc atggaagcac cgagggaact gccggagagc ggggtagcgg cctcgggctg
     1261 attctctgca aggagttcgt ggagaagcat ggggggcgga tttggttcga gagctcgccc
     1321 ggagagggaa ccgtcttctt tttctcgttg cccgacagac tgaacggcga tcctgcggaa
     1381 gagcgcggag tgtgacggaa cggaaggagg tcgc
//
LOCUS       DAOPKU010000702         1407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_111118_length_1407_cov_2.774408, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000702 DAOPKU010000000
VERSION     DAOPKU010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111118_length_1407_cov_2.774408"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PK849_15085"
     CDS             complement(<1..188)
                     /locus_tag="PK849_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67506.1"
                     /translation="MSVYSDEELERMLDDLESDLVERKESFRGDVPKKARQAVCAFSN
                     DLPFDLHPVPEASLNDLS"
     gene            579..1253
                     /locus_tag="PK849_15090"
     CDS             579..1253
                     /locus_tag="PK849_15090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPE67507.1"
                     /translation="MDYGKRKHLRIVVEAERGVEVRAPRSASFAVVTEAVQRRAGWIA
                     GALERVHNAALLHPKPSYSEGEEHLFLGVLYPLSIRTGRGLGELSGGRLALRVPSVPA
                     DGEAVKRSLNRWYASQAESVFAASMARCLPRCAAFVQCAPPLKARFLRARWGSWSSAR
                     GVVLNTHLLKAPPECIDYVLLHELCHVRHPNHGKGFYELLESVSPEWKELKKKLEETV
                     HLRLFA"
BASE COUNT          283 a          393 c          451 g          280 t
ORIGIN      
        1 ctggaaagat cgttcagcga ggcctccgga acagggtgga gatcgaacgg caggtcgttg
       61 gagaatgcgc agacagcctg ccgcgctttt ttcggcacat ccccgcggaa ggattccttt
      121 cgttcaacaa gatcggattc gaggtcgtcg agcatccgct caagctcctc atccgaatag
      181 accgacatcg ccgcaccacc tttcagcgaa ttcagggcga tgaaacgctt cgccgcccga
      241 ttccgttttc tggaatatct ttttcccgtt tctttataat agcatgaacg cttcgatgtg
      301 cggacacacg atgaagaggc ggaaaggccc atctcgagaa gcctttcgga gtcttcggat
      361 atccttgaaa gagccgagac ccgttccgtg gtattatgga cgtacctgca ctttgagtat
      421 ctttctgctt ctgcggaaaa ttctacaacg ccggacggca aaggagtaat cgtgtacgct
      481 cactatacta cgaacttatt gacgatgggc gaagaatgag cgcgaggaag ccgcgggagg
      541 gctcgctggc ggtttgcggg cgggagattt cgttcactgt ggattatggg aagcgcaagc
      601 atctgcgtat tgtcgtggaa gcggagcggg gggtggaggt gcgcgctccc cgctccgcga
      661 gtttcgccgt cgtgacggag gcggtgcagc ggcgggccgg gtggatcgcc ggggcgctcg
      721 agcgcgtcca taacgccgcg ctgctccacc cgaagccatc gtactccgag ggggaggagc
      781 atctctttct gggagtcctc tacccccttt ccatacggac ggggcggggc ttgggcgaac
      841 tctccggcgg gaggctggcg ctgcgggtac cgtcggttcc tgcggacggc gaggcagtga
      901 agcgctcgct caacaggtgg tacgcgtcgc aggcggagag cgtcttcgcg gcaagcatgg
      961 cgcgctgcct cccgcgttgc gcggcgtttg tccagtgcgc gcccccgttg aaggcgaggt
     1021 ttcttcgggc gcgctgggga agctggtcga gcgcgcgcgg cgtcgtgctg aacacgcatc
     1081 tgctgaaggc cccgcccgag tgcatcgact acgtcctgct ccacgaactc tgccacgtcc
     1141 gccacccgaa ccacggcaaa ggcttctacg agctgctgga aagcgtgtcc cccgagtgga
     1201 aggaactgaa gaagaagctg gaggagactg tccacctgcg cctctttgcg tgaaggggac
     1261 acttacaaca ggtcaaggcg cccttctttc catattcaac aagaatcgtg attcgaagtg
     1321 aatcgaatcg gaaagaacgg cgagtggaaa acgaacgccg gaggcactgt gattcccggg
     1381 gcgaacgatg ttttggcaaa caacaca
//
LOCUS       DAOPKU010000703         1403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_111547_length_1403_cov_4.172107, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000703 DAOPKU010000000
VERSION     DAOPKU010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111547_length_1403_cov_4.172107"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PK849_15095"
     CDS             complement(<1..1071)
                     /locus_tag="PK849_15095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HPE67508.1"
                     /translation="MRNKHVVLKEYFGFGGFRSGQEEVIDALLDGRDAVGIMPTGAGK
                     SLCFQIPSLLFEGVSLVVSPLISLMKDQVSALRQSGVAAAFLNSTLTFPQMKTVLRNA
                     RAGKYTLLYVAPERLATEDFLEFARDASIPFVAVDEAHCVSQWGQDFRPSYLKIGEFV
                     RSLPLRPVLGAFTATATPQVRDDIVQLLNLRAPHVVTTGFDRKNLYFEVQSPKSKPAA
                     LKEFLRSRKEKSGIVYCLTRKTVDEVCERLCEAGYSATRYHAGLTQEERRENQDRFQA
                     DDARIMVATNAFGMGIDKSNVAYVLHYNMPKNMESYYQEAGRAGRDGSPAECVLFYGG
                     QDVFTNQFFIEKERENEELDEET"
     gene            complement(1105..>1403)
                     /locus_tag="PK849_15100"
     CDS             complement(1105..>1403)
                     /locus_tag="PK849_15100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HPE67509.1"
                     /translation="NPIGYVLQLEAVSNFFDDDVLLLAATIVRSIIQGHPLQDGNKRL
                     GMVLGTYFLALNGFSLTASDEDYFETAIGLASGRIDERRLLDWIRECAVSVGEA"
BASE COUNT          258 a          439 c          434 g          272 t
ORIGIN      
        1 cgtctcctcg tcgagttctt cgttctcgcg ctctttttct atgaagaact ggtttgtgaa
       61 cacgtcctgc ccaccgtaga agagcacgca ctccgcaggg gagccgtcac gtccggcgcg
      121 gcccgcctcc tgatagtagc tctccatgtt cttcggcatg ttgtagtgga gcacgtacgc
      181 gacgttcgac ttgtcgatcc ccatgccgaa ggcgttcgtc gccaccatga tccgcgcgtc
      241 gtcggcctgg aagcggtcct ggttttcccg gcgctcttcc tgagtcagtc cggcgtggta
      301 gcgcgtcgcc gagtaccccg cctcgcagag cctctcgcag acctcgtcga ccgtcttgcg
      361 cgtgaggcag tagacgatgc cgctcttctc cttgcggctg cgaagaaact ccttcagcgc
      421 cgccggcttg ctcttcggcg actgcacctc gaagtagagg ttcttgcggt cgaaacctgt
      481 cgtgacgacg tgcggcgcgc ggaggttcag caactggacg atgtcgtcgc gcacctgcgg
      541 cgtcgccgta gcggtgaagg ccccgagcac ggggcggagc gggagggagc ggacgaactc
      601 gccgatcttg aggtagctcg ggcggaagtc ctgcccccac tgcgagacgc agtgcgcctc
      661 gtcgacggcg acgaacggaa tggaggcgtc gcgtgcgaac tcgagaaagt cctccgtcgc
      721 aaggcgctcc ggcgcgacgt agagcagcgt gtacttcccg gcgcgggcgt tgcggaggac
      781 ggtcttcatc tgggggaacg tgagcgtgct gttgagaaac gcggccgcga cgcccgactg
      841 gcggagtgcg ctcacctggt ccttcatcag ggagatcagg ggggagacga ccagagagac
      901 cccctcgaag aggagcgacg ggatctggaa gcagagcgac tttcccgccc ccgtcggcat
      961 gatccccacc gcgtcgcgcc cgtcgagcag cgcgtcgata acctcctcct gcccggagcg
     1021 gaagcccccg aagccgaaat actccttcag cacgacgtgc ttgttgcgca ttgactcatt
     1081 cctttccgca gctgtcgaaa cagactatgc ctcgcctacg gacacggcac attcccgtat
     1141 ccagtcgagc agccgtcgtt catcaatacg accgctcgcc aggcctatgg ctgtttcaaa
     1201 atagtcctca tcggacgcag tgagggaaaa cccgttcagt gcgagaaagt atgttcccag
     1261 caccatcccg agacgcttgt ttccgtcctg cagagggtgt ccttggatga ttgaacggac
     1321 tattgtggcg gcaagcaaaa gtacatcgtc gtcgaaaaaa ttcgagacgg cctcaagctg
     1381 aagaacgtac ccgatggggt tat
//
LOCUS       DAOPKU010000704         1403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_111552_length_1403_cov_4.077151, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000704 DAOPKU010000000
VERSION     DAOPKU010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111552_length_1403_cov_4.077151"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="PK849_15105"
     CDS             complement(<1..1217)
                     /locus_tag="PK849_15105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPE67510.1"
                     /translation="MSLLSGGMGWRRKGGTKMRAHSRLAAATVAVSMLAGAGGGIASA
                     DAKSSLAESMNLAPRRNADGNYNIIFITTDQERYMQDYPAGTAYEARELLASLGATFE
                     KHYACATMSTSSRSVIFTGKHITDTKMIDNTDFPWQGPLSKEMTTVGDIMRTAGFYSA
                     LKGKWHLGNASTLEDVEDELTDLDEHGFSDWDVDGDYIGTMWEGYNKDEEIVTDTVAW
                     LRNVGAVQNAAGKSFFLAANLINPHDIMYFCVDEEFVGPVPVGGAPDDPLYTKTYDEP
                     LPSSWNRDVSDGTVPAAVSIFKNFMNKQTGVLSTEEEWKETQDYYFNCIQDNDNHLID
                     LLLAVRDMGMLDNSIIFFTSDHGEMGGAHGLKGKGGLLFEQNLHVPLVVYHPDYEGGR
                     SVNAVTSHIDLATT"
BASE COUNT          239 a          437 c          414 g          313 t
ORIGIN      
        1 gtcgtcgcga ggtcgatatg gctggtgacc gcgttcacgg aacgacctcc ctcgtagtcc
       61 gggtggtaga cgacgagcgg gacgtggaga ttctgttcga agagcaggcc gcccttcccc
      121 ttcaagccgt gcgcgccgcc catctctccg tggtcggagg tgaagaagat gatcgagttg
      181 tccagcatcc ccatatccct cacggccaga aggaggtcga tcaggtggtt gtcgttgtcc
      241 tggatgcagt tgaagtagta gtcctgcgtc tccttccact cctcttcggt gctcagaaca
      301 ccggtctgct tgttcatgaa gtttttgaag atggaaacag ccgcaggaac cgtcccgtcg
      361 ctcacatccc tgttccatga ggacggcagc ggctcgtcgt aggtctttgt gtacaggggg
      421 tcgtccggag ccccgccgac gggaacggga ccgacgaact cctcgtcgac gcagaagtac
      481 atgatgtcgt gcggattgat caggttggcc gcgaggaaga aggacttccc ggcggcattt
      541 tgaacggccc ccacgttgcg aagccatgcg accgtatcgg tgacgatctc ttcgtccttg
      601 ttgtacccct cccacatcgt gccgatatag tcgccgtcga catcccagtc ggagaatccg
      661 tgctcgtcaa ggtcggtcag ctcgtcctcc acatcttcaa gcgtgcttgc gttccccagg
      721 tgccacttcc ccttgagcgc gctgtagaat cccgccgtgc gcattatgtc gccgactgtc
      781 gtcatctcct tggaaagagg gccctgccac ggaaaatccg tgttgtcgat catcttcgta
      841 tcggtgatgt gttttcctgt gaagatgacg gaacgggacg aggtggacat cgtggcgcag
      901 gcgtagtgct tctcgaaggt ggcgccgagc gaggccagca gctcccgcgc ttcgtaggcc
      961 gtacccgcgg ggtagtcctg catgtagcgt tcctggtcgg tggtgatgaa gatgatgttg
     1021 tagttcccgt cggcgttccg tctcggagca aggttcatac tctccgcaag cgaagacttc
     1081 gcatccgcgg aggctatgcc gcctcccgcg cccgcgagca tggaaacggc aacagtagcg
     1141 gccgccaatc tagagtgtgc acgcatcttc gttcctccct ttctacgcca tcccatgccg
     1201 ccggacagca acgacatgcc ccggcctccg cgccctgttc agcgactcag gccccactcc
     1261 ttccgccact gcttctgcag ctcctgcgcc cgtttcttgt cgcgggagga gatggtcttt
     1321 tccagctctt cgagcatttc gtcggcgttc gccaccgagc gtttcgccgc cagatccagg
     1381 taggcccacg ccatgacggg gtc
//
LOCUS       DAOPKU010000705         1402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_111708_length_1402_cov_2.731997, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000705 DAOPKU010000000
VERSION     DAOPKU010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111708_length_1402_cov_2.731997"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /gene="gspG"
                     /locus_tag="PK849_15110"
     CDS             complement(<1..236)
                     /gene="gspG"
                     /locus_tag="PK849_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HPE67511.1"
                     /translation="MTIFFKGKRRKGFTLVEVLVVVVILGLLAALVAPKVVGQGEEAK
                     RTATSVQIREIEQALEMYRLDNSMYPTTAQGLEAL"
     gene            complement(271..>1402)
                     /locus_tag="PK849_15115"
     CDS             complement(271..>1402)
                     /locus_tag="PK849_15115"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPE67512.1"
                     /translation="AYDKSGKLRKGIIDAPSSTRAGELLAEQNLVAVEIIEGAAESVK
                     KGKVFGLAYHSLFCKGLSSYLNRGVPLAEALRFLSRHSNDRRVSAACLHLHESVQGGK
                     RLSAALEETGLFRDDLVRIVDSGERTSALSGVLDQVARQYAMEDKQRRKIRSALTYPV
                     AMSIIGIGVVVFLLTYVVPKLTELFSEMGQQLPLPTRILLAITAFLRSWGLPLLLVLL
                     LVILYIRRRKKKFTLPFFRRVRESITLSLVMSHLSTLLSAGIPLVQALNMASAMDSNA
                     QRWLEAARLVKEGFRFDRALEKQGAFSEEVIYILRIGEMGGDLPGAIKQVSDMKWEEA
                     LESMERLSTLAEPVLVLFLGFSVGFVVLAILLPIFELSSLAG"
BASE COUNT          338 a          413 c          363 g          288 t
ORIGIN      
        1 agcgcctcaa gcccctgcgc cgtcgtcggg tacatgctgt tatcgagcct gtacatttcc
       61 aaggcctgct cgatctcgcg aatttggacc gaagtcgcgg tccgtttcgc ctcttcaccc
      121 tgtcctacaa ccttcggggc aacaagagca gcaagaagac cgaggataac gacgaccaca
      181 agaacttcaa caagagtaaa gccttttctc cgcttgcctt tgaaaaatat tgtcatattt
      241 tcctcctcca tttcctttcc tcaaattctt ctaacctgca aggctcgaca attcgaatat
      301 cgggagaaga atagccagaa cgacaaaccc caccgaaaag cccaggaaaa gcaccaggac
      361 cggctcagcc agcgtagaga gtcgttccat actctcaagc gcttcctccc atttcatatc
      421 cgagacctgt ttgatagccc cgggcaggtc gccgcccatc tcgccaatac gaagtatgta
      481 gatcacctct tccgagaaag cgccctgctt ctcaagggcc cggtcgaaac ggaagccttc
      541 cttgaccagg cgggctgctt ccagccagcg ttgtgcattt gaatccatcg cagaggccat
      601 gttgagcgcc tgaacaaggg gaatgccggc tgaaaggagc gtgctgagat gcgacatcac
      661 gagtgaaagc gtaatgctct cccgtacccg ccggaaaaaa ggaagggtaa atttcttctt
      721 tctgcgccgg atgtacagaa taacgagcag cagcacgagc aacagcggaa gcccccacga
      781 acggagaaac gccgtaatcg ccagcagtat tctcgtcgga agcggaagct gttgtcccat
      841 ttccgaaaaa agttccgtca gcttcgggac tacgtaggtc aacaggaaaa caaccacacc
      901 gatgccaata atgctcatgg caaccggata ggtgagagcc gaacggatct tgcgtcgctg
      961 tttgtcctcc atagcgtact ggcgcgctac ctgatcgaga actccgctca gggccgaggt
     1021 ccgttcaccc gagtcgacaa tacgaacaag gtcgtcccgg aagagtcctg tctcctcgag
     1081 cgccgcggag agacgttttc ctccctgcac gctctcgtgc agatggagac aggccgccga
     1141 gacacgacga tcattggagt ggcggctgag gaagcgcaat gcttccgcga ggggcactcc
     1201 cctgttgagg taagaagaga gacctttgca gaaaagtgag tgatacgcga ggccgaacac
     1261 cttgcctttc tttaccgatt cggccgcccc ttcgatgatc tcaacggcca ccaggttctg
     1321 ctctgcgagg agttctcctg cgcgggtgct ggacggcgcg tcgataatcc ccttgcggag
     1381 ctttcccgat ttatcgtatg ca
//
LOCUS       DAOPKU010000706         1401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_111810_length_1401_cov_3.038633, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000706 DAOPKU010000000
VERSION     DAOPKU010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111810_length_1401_cov_3.038633"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PK849_15120"
     CDS             <1..274
                     /locus_tag="PK849_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67513.1"
                     /translation="AGLAQVPGSDAPVLAAIQADMIRDIAAYYNEEITTVRALSLVAT
                     FSATAVGRFVSQWLVGWIPGLGNAINASTAAAITEGIGWAADAYFS"
     gene            complement(354..>1401)
                     /locus_tag="PK849_15125"
     CDS             complement(354..>1401)
                     /locus_tag="PK849_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPE67514.1"
                     /translation="RMERLLTGEKRSDPEDLFRLEDVLKEAIRSWLGRCGKGEREFSK
                     PVDWKASLDAHLSESGYVSLFADPSTRRMALEVKEAIHSLKSRAEAIQKSAEKLPLSE
                     TNFKEMANKGWSVFAGRIPSVLLLGESGSGKTLLAKWIGESLLPKQFERVNISAIQSS
                     LVDGELFGAAKGAFTDHPFDTQGLFLSQAGGVVFLDEIGDMEPACQTRLLTFIDDGLV
                     RPLGWHGQPFAAPLLLVAATNRPLKEWVSSKSASFREDLFYRFDHVIELPPLRERRGD
                     LRLLISVTLQDEDVNAGYAQGKGVRRISLDAIEALERMEMPGNFRELRGVLKRACFAA
                     RREKAETLCLRHFA"
BASE COUNT          301 a          440 c          363 g          297 t
ORIGIN      
        1 cgcgggcttg gcgcaggttc ccggctccga cgctcctgtg cttgcggcga ttcaagcaga
       61 catgattaga gatatcgccg cctattacaa cgaagaaatc acaacagtaa gggcattatc
      121 tctggtggcc actttctcgg cgaccgcagt agggcgcttc gtttcccagt ggcttgtggg
      181 ctggattccc gggctgggca acgcgatcaa cgcaagcacc gccgccgcaa taaccgaggg
      241 tatcgggtgg gctgccgacg cctacttctc gtgagaatcc ggtcgcccct gcggctacga
      301 acgcaggggc aacggatcgc aacacagcag cacgtttttg aacaccgtcg gactcaagca
      361 aagtgccgca gacagagtgt ttcggctttc tctctgcgtg cggcgaagca ggcgcgtttc
      421 agaacacctc gaagctccct gaagttgccc ggcatctcca tacgttccaa cgcttcaatt
      481 gcgtcgagcg agatgcgccg aacccctttc ccctgcgcat atcccgcgtt cacatcttcg
      541 tcctgcaacg tgacgctgat aagtagccga agatctccgc gccgttcgcg gagcggagga
      601 agttcgatca cgtgatcgaa ccggtagaac aggtcttcac ggaacgaggc gcttttcgac
      661 gaaacccact cttttaacgg ccggttcgtg gcggccacca gaagaagcgg cgccgcaaac
      721 ggctggccgt gccagccgag cggccgcaca agcccgtcgt cgatgaatgt gagcaggcgt
      781 gtttggcagg caggttccat atcgccgatc tcgtccagaa agacaactcc gcctgcttgc
      841 gacaagaaaa ggccttgagt atcgaacgga tgatccgtaa acgctccctt cgcggcgccg
      901 aacagctccc catccaccag agaagactgt atagcggaaa tattgacccg ttcaaactgc
      961 ttcggcagaa gcgactctcc gatccacttc gcaagcagcg tctttcccga acccgattct
     1021 ccaagcagaa ggaccgaagg aatcctgccc gcgaaaacgc tccatccttt attcgccatt
     1081 tccttgaagt ttgtttcact caaagggagt ttttcagcgc tcttttggat tgcctccgcc
     1141 ctacttttca gggaatgaat ggcctccttg acctccagcg ccattctgcg tgtggaaggg
     1201 tcggcgaaaa gactcacata cccgctttct gaaaggtgcg catcaagaga agccttccag
     1261 tccaccggct tcgaaaactc tctctcccct ttcccgcacc ttccgagcca ggatcttatg
     1321 gcctccttca atacatcctc aagacgaaac aagtcttcgg ggtcgctccg tttctccccg
     1381 gtcagcagtc tctccatcct g
//
LOCUS       DAOPKU010000707         1395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_112577_length_1395_cov_2.402985, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000707 DAOPKU010000000
VERSION     DAOPKU010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112577_length_1395_cov_2.402985"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..1273)
                     /locus_tag="PK849_15130"
     CDS             complement(344..1273)
                     /locus_tag="PK849_15130"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HPE67515.1"
                     /translation="MRIVSVSRRTDIPAFYSPWLFRRLKDGYVLVRNPRNAKQTRFVS
                     LKPDDVHCFVFWTKDARPMFGRLDELEAHESAFYFMWTVTPYGPEIEPGFPRKEEIVS
                     AFRQLSGRIGPRRTIWRYDPVVLDDSFPVRKHLEAFARMCASLDGATERCIVSFVDMY
                     RKLRGKGITEVGEEDMRIIARGFSEIASSHGIAVQTCCEAADFGAEGCFPGGCIDAAL
                     VEELSGKPVPEKKDAGQRPLCRCAPSVDIGAYDTCPGGCAYCYATQSAAAVRANASRH
                     DPASPLLSGTPSPEEQQLIERSRTPQQRRLALF"
     gene            complement(1286..1393)
                     /locus_tag="PK849_15135"
     CDS             complement(1286..1393)
                     /locus_tag="PK849_15135"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPE67516.1"
                     /translation="MPCHRVINTGGKLGGYGGGLDLKKTLLELEARNRP"
BASE COUNT          244 a          441 c          437 g          273 t
ORIGIN      
        1 tgcgtacggc ttcccggaca agagggcctc gtccgccctg tcctgttcca gcccttccag
       61 gtatttcaaa aacaggagcc acgaagactg ctccgtgtag tcgagttccg tagtgcatcc
      121 cgcctctttc ctgaggacgt cgtcgatatt ccggaaggtc tgctcgaaca tgcgtttgcc
      181 tcttcctttc atgtgtgaag accgccggga atgttccgcc aagcgggtgt catttattcc
      241 gtaatgtcat tagcgtcacg atagcattga tgagaagggt tgtcaatttt gctccgtcaa
      301 gaagcaaggg gcgagccggt gacgggaggg cgctgccgat gcattaaaag agagcgaggc
      361 gcctctgctg cggtgtgcgc gaccgttcta tgagctgctg ctcttcgggc gacggggtcc
      421 cgctcaacag gggagaggcg ggatcatggc gcgaggcatt cgcgcggacg gccgccgcgc
      481 tctgtgtggc gtagcagtac gcgcacccgc cggggcaggt gtcgtacgcg ccgatgtcca
      541 cgctcggcgc gcaccggcag agggggcgct gcccggcgtc ctttttctcc ggaacgggct
      601 tccccgaaag ctcctcgacg agcgcggcat cgatgcaccc tccggggaaa cacccctccg
      661 ctccgaagtc cgcggcctcg cagcaggtct gcactgctat tccgtgcgag ctggcaattt
      721 cggagaaacc acgcgcgata atccgcatgt cttcctcgcc gacctcggtt atcccctttc
      781 cgcgcagctt ccggtacatg tcgacgaagc tgacgataca ccgctccgtc gccccatcga
      841 gcgacgcgca catgcgggcg aacgcttcca ggtgcttccg gacggggaac gagtcgtcga
      901 ggacaacggg gtcgtacctc cagatcgtcc gccgcgggcc gattcgtccg ctcagctgcc
      961 ggaaggcgga cacgatctcc tctttccggg gaaagccggg ttcgatctcc gggccgtacg
     1021 gcgtgaccgt ccacataaag tagaacgcgc tctcgtgcgc ctccagttcg tcgaggcgtc
     1081 cgaacatcgg ccgggcgtcc ttggtccaga agacgaagca gtgcacatca tccggcttca
     1141 gcgagacgaa gcgcgtctgc ttcgcgttcc gcggattgcg gacgaggaca tacccatcct
     1201 tcaaacggcg gaacagccac ggggagtaga atgccgggat gtcggtccgt ctgctcacgc
     1261 tgactatgcg cacacgaacg acatcctacg gtctgttgcg ggcttccagc tcgaggagcg
     1321 ttttcttcag gtcgagcccc ccgccgtatc cgccgagctt gccgccggtg ttgatgacgc
     1381 ggtggcaggg gatga
//
LOCUS       DAOPKU010000708         1394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_112638_length_1394_cov_4.127707, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000708 DAOPKU010000000
VERSION     DAOPKU010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112638_length_1394_cov_4.127707"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PK849_15140"
     CDS             complement(<1..472)
                     /locus_tag="PK849_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67517.1"
                     /translation="MEYGMIRFTPEDSYVVEDILIVLSRFFPARPVKGYSVEVADSLR
                     GGLSFIEGDGDTDSLGKGLLLSLARVYVSMDEEGGSVLIAADSMEERARWISSQWKSR
                     SGKNFNGVIHHTYQGNGWSLVGIAAPGWMVLFDDETENAEQDWLEADLRVAYLPP"
     gene            complement(502..>1394)
                     /locus_tag="PK849_15145"
     CDS             complement(502..>1394)
                     /locus_tag="PK849_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HPE67518.1"
                     /translation="RLFKCLRKHILFDQKVEITVEANPDSLSESHVRLWKEHGVSRIS
                     VGVQSFIDGELRWLGRLHDADRARMALAMCVHSGISVSADFMFGLAGQSLRSFSTSIA
                     EAIALGAEHLSLYQLSIDETSRWSDNPPEGRKDGYPFYRWSQWYLPKKGFAQYEISSF
                     ARPGKECRHNSAYWTNGAVLALGAGAWGYADGIRYRNERTLTGYAEAIRRNGHSVAEA
                     EIPDACKRAHEAAILMLRTARGISFEAFASRYGEETLSSLLRTLEENAPPDCFVRSDA
                     SFALSPKGMRVANALWSLIV"
BASE COUNT          333 a          411 c          352 g          298 t
ORIGIN      
        1 cagggggaag gtacgcaacg cgaagatcgg cctccaacca gtcctgttca gcattttcag
       61 tttcatcatc aaagagcacc atccatcccg gcgctgcgat tcccaccaga ctccatccgt
      121 tcccctgata ggtatggtga atgacgccgt tgaaattttt ccctgagcgt gatttccact
      181 gcgagctgat ccagcgagcc cgttcttcca ttgagtcggc ggcgatcaaa acgctccccc
      241 cctcttcgtc cattgagaca tagacgcgag caagtgaaag gagaagcccc ttgccaaggg
      301 agtctgtatc cccgtccccc tcaataaaag agagccctcc gcgcagcgaa tcagccacct
      361 caacggaata ccccttcaca gggcgggcgg ggaaaaagcg cgagagtaca atcagaatat
      421 cctctacgac ataagaatcc tcgggagtga aacgtatcat gccgtattcc atcggcagca
      481 tcttcctttc ctgaacgctg attacacaat aaggctccac agggcgtttg cgaccctcat
      541 ccccttcggg gagagcgcaa aagatgcgtc gctccgaaca aaacaatcgg gaggcgcatt
      601 ctcctccaga gtacgaagaa gtgacgagag tgtttcttct ccgtaccgcg aggcaaaagc
      661 ttcgaagctg atacctctgg cagtccgaag catcaggatt gcagcttcat gcgcacgctt
      721 acaagcatcc ggaatttcag cttcggcaac cgagtgtccg ttgcgccgga ttgcttcagc
      781 atatcctgta agcgttcgtt cgttgcgata gcgaatacca tccgcgtacc cccacgctcc
      841 cgcgccaaga gcgagcaccg cgccgtttgt ccagtaggcg ctgttgtgcc ggcattcctt
      901 ccctggccgg gcaaagcttg agatttcata ctgggcaaaa ccttttttcg gaagatacca
      961 ctggctccac ctgtagaacg ggtatccgtc tttccgccct tcaggcggat tgtcggacca
     1021 tctgctggtt tcatcgatcg agagctgata cagcgagaga tgctcagctc cgagagcgat
     1081 tgcctccgcg atcgaggtgg aaaaggagcg taaagactga ccggcaaggc cgaacataaa
     1141 atctgcgctc accgaaatgc ctgagtgaac gcacattgcc aacgccatcc tggcgcggtc
     1201 cgcgtcatgg agtcgtccaa gccagcgaag ttcgccgtca ataaagcttt gcacaccgac
     1261 gctgattctg gagaccccat gctctttcca gagacgcacg tgactttcag aaagggagtc
     1321 gggatttgcc tccacggtga tctctacctt ttgatcgaaa aggatatgct tgcgtaaaca
     1381 tttgaaaagc cgct
//
LOCUS       DAOPKU010000709         1393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_112772_length_1393_cov_3.956652, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000709 DAOPKU010000000
VERSION     DAOPKU010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112772_length_1393_cov_3.956652"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..820)
                     /locus_tag="PK849_15150"
     CDS             complement(158..820)
                     /locus_tag="PK849_15150"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE67519.1"
                     /translation="MKVPTMVEVIQAELKKRVLDGEFVPGDRLNVDELARVLNVSKTP
                     IREALGRLAREGIVQSKPRIGWRVSLLSMEEFFQYQETQHVIKMYLAEQIFPYLDKID
                     IPKLEKMNGNMHHFVETRQFERMLEENDRFHMEIFGVYPNKVLLEYLEEVNNTIRLQR
                     IHMMEKQLLSPESPLLQDAPKDHEEIIDAIKSGDRTRIIETSAKHQATILEALKRPGK
                     VS"
     gene            970..>1393
                     /gene="dctP"
                     /locus_tag="PK849_15155"
     CDS             970..>1393
                     /gene="dctP"
                     /locus_tag="PK849_15155"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE67520.1"
                     /translation="MKRVLMCAVCVVGVLLSVAFPAAAEKYVIKIGNVTAPDHPLNVS
                     FEKMAALMNEKSGGRIEATVYPSAQLGSLRALAEGLQLGTVEMGTQSPGGLASFLPLM
                     GVLELPYIYRSNEHVYKTVDGPIGLELNEKIRAKTGIRI"
BASE COUNT          283 a          385 c          403 g          322 t
ORIGIN      
        1 caccttgaac ggcgagccct ctttgctctt ctcaagctgt ttctgaagct ccaacgtttt
       61 ccataacgtg ttgcccgatc cggagaccag tccggccaga cgcagtttcc ccctcagggg
      121 actccacaga gggtgtgtca cagcgaattc ctcccgttca gctcactttc cccgggcgtt
      181 tgagcgcttc gaggatggtg gcctgatgtt ttgccgaagt ttctataata cgcgtcctgt
      241 cgccgctctt gatggcgtcg atgatctctt cgtggtcctt cggcgcgtcc tgaagcagcg
      301 ggctctcggg agagaggagc tgcttttcca tcatatggat gcgctgcagg cgtatcgtgt
      361 tgttgacctc ttcgaggtac tccaggagca ccttgttggg gtagacgccg aaaatctcca
      421 tgtggaagcg gtcgttctcc tcaagcatcc gctcgaactg cctcgtctcg acaaagtgat
      481 gcatgttgcc gttcatcttc tccagcttcg gaatgtcgat cttgtcgagg taggggaaga
      541 tctgttccgc aaggtacatc ttgatcacgt gctgcgtctc ctgatactga aagaactcct
      601 ccatagagag gagcgagacg cgccacccta tccggggctt cgactgcacg atcccctcac
      661 gggccaggcg tccgagcgcc tcgcggatcg gcgtcttgct cacgttgaga acgcgcgcga
      721 gttcgtccac gttgagtctg tcgccaggga caaactcgcc gtcgaggacg cgctttttca
      781 gctccgcctg aatcacttcc accatggttg ggaccttcat ggaaaacgcc cctttcccga
      841 tacgggtaaa tctgatatag tatccgatat gcaagatagt atcttgaaac agatttcttg
      901 tcaacgaagg agaagcggcg gacagtgttc caaacggtgt gttccatgat atgaaaggag
      961 atggatgcga tgaaaagagt tctgatgtgc gcggtatgcg tggtcggcgt gcttctatcg
     1021 gtagcgttcc cggcggcggc ggagaagtat gtcatcaaga ttggcaatgt aacggcgcct
     1081 gatcacccgt tgaacgtctc gttcgagaag atggcggccc tgatgaacga gaagagcggc
     1141 gggcgcatag aggccacggt ctatcccagc gcgcagcttg gcagcctccg cgctctcgcc
     1201 gaagggttgc agctcgggac ggtcgaaatg ggaacccaga gtccgggagg tctggcatcg
     1261 ttccttccgc tcatgggcgt gctggagctg ccctatattt acaggagcaa cgaacacgtg
     1321 tacaagacgg tcgacggacc gatcggcctc gaactcaacg aaaaaatccg tgcgaagacg
     1381 ggcatacgca ttc
//
LOCUS       DAOPKU010000710         1390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_113121_length_1390_cov_4.801498, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000710 DAOPKU010000000
VERSION     DAOPKU010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113121_length_1390_cov_4.801498"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK849_15160"
     CDS             <1..416
                     /locus_tag="PK849_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67521.1"
                     /translation="LLTFFLYPVVWLFLRLRTFNSMSEKTRIHPVLSGLFACAAVALA
                     VILNGDSLIGPAVPQQAIDTAISYIFLANFILSAVLTFTLRRILREYAARMDKSPLAA
                     KIVARSEVFAFLFQFLYIQHHINLLIDAGLVERTN"
     gene            complement(497..>1390)
                     /locus_tag="PK849_15165"
     CDS             complement(497..>1390)
                     /locus_tag="PK849_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPE67522.1"
                     /translation="SPELAGEGYPFDYLQNSAVHPGEPLFVSHLSLDGLWAWCDTSYA
                     SGWVELRDLAFVDDAAKVRWTALPLAAIVRENALLRDGTESLFRAKVGALLPLLRRGI
                     GEHVLIVPVRGQNGLMTERAIRISAEEAVPAPLVPTTWTLAAVAEELRGELYGWGGFL
                     GNRDCSATTRDTLLPFGVWLPRNSRFQAESGRFLTLEGLSNEEKKELLKREGVPFLTL
                     VGLPGHIMLYVGTHRGEPLVFHNMWGIRTERKGVEGRYVLGRTAVTTLDLGEDLPEHA
                     PGKLLIDRINRLGFPAEDGVQ"
BASE COUNT          256 a          482 c          376 g          276 t
ORIGIN      
        1 cactgctgac gttttttctc taccctgtcg tctggctctt tctccggctt cggaccttta
       61 attccatgtc ggagaagacg cgtatccatc ctgttctttc cggccttttc gcatgcgccg
      121 cagtcgccct ggcagtgatt ctcaacgggg actcgctcat cggaccggca gtaccgcagc
      181 aagccatcga cactgccatc agctatattt tccttgcgaa cttcattctt tcggctgttc
      241 tcaccttcac cctgcggcgg attctgaggg agtacgcggc acgcatggac aagagccccc
      301 tggcagccaa aatcgtcgcc cgctcggagg tcttcgcatt cctctttcaa ttcctgtata
      361 tccagcacca catcaacctg ctgatcgacg cagggctcgt tgaacggacg aactgattct
      421 cttccgcccc cggcggaacg gccggtctga cgcaagggag cctcgtatga ccacatccgc
      481 cgaggctccc ttgtgtttat tgcactccgt cctccgcggg aaagcccaga cggttgatcc
      541 tgtcgataag cagctttccc ggagcatgct cgggaagatc ctccccgaga tcgagcgtgg
      601 tcaccgccgt ccgtccgaga acataccgcc cttcaacacc ctttcgctcc gtccgtatcc
      661 cccacatgtt atggaagacg agcggctccc ccctgtgcgt tccgacgtag agcatgatat
      721 gccccggaag accgaccagc gtcagaaagg gaaccccttc ccgcttcaga agttctttct
      781 tttcctcgtt cgagagtcct tcgagcgtga gaaatcgccc gctctccgcc tgaaaacgcg
      841 agttgcgggg aagccagacg ccgaagggaa gcagtgtatc tcgcgttgtt gcgctgcagt
      901 cgcggtttcc taaaaaaccg ccccagccgt aaagttcgcc gcgcagctcc tccgccaccg
      961 cggcaagcgt ccacgtcgtg gggacgagcg gcgcggggac ggcctcctcg gcggagattc
     1021 ggattgcgcg ctcggtcatc agaccgttct gtcctcgcac cgggacaatg agcacatgct
     1081 ccccgatgcc ccgccgcagc agcggcagca gcgcgccgac cttcgcccgg aagagcgact
     1141 ccgtcccgtc gcggagcagc gcgttctccc gcacgatcgc agcgagaggc agcgccgtcc
     1201 acctgacctt cgcggcatcg tcgacgaagg caagatcccg aagttccacc caccccgacg
     1261 cataggaggt gtcgcaccac gcccacagac cgtccagcga gagatgcgaa acgaagagcg
     1321 gctctcccgg gtgcaccgcg ctgttctgca gatagtcgaa cgggtacccc tcccccgcca
     1381 gttcggggga
//
LOCUS       DAOPKU010000711         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_113291_length_1389_cov_3.047976, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000711 DAOPKU010000000
VERSION     DAOPKU010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113291_length_1389_cov_3.047976"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PK849_15170"
     CDS             <1..517
                     /locus_tag="PK849_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67523.1"
                     /translation="LINQFLSPLSNRRRDGYGVNFEGRSRFLQEIARAVRTRWPEEKP
                     LFLRISAEDYVEGGMTTGQMVRIVDRVTKEVDVVHVSSGGVTPVAPPVHPGYQIPFAE
                     QIKRECEVPTIAVGLITSLDMIEEALCNQRADLVALGRELLRNPFFPLNEARKRGIEL
                     PWPEQYRRAFS"
     gene            complement(568..>1389)
                     /locus_tag="PK849_15175"
     CDS             complement(568..>1389)
                     /locus_tag="PK849_15175"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPE67524.1"
                     /translation="KSPPEILSEMVAAAVSIARAIGVRGFLNIQFAMKEGVLYVLEVN
                     PRASRTVPFLSKASGVDLVRAAVRLWEGKDLEAQGLVRNGYGEGRCLTGWAVKEAVFS
                     FDRFASVDPLLGPEMRSTGEVMGSGESVGEAYAKAQAACGTVLPTKGTVFISVNDPDK
                     PTILPVAKRLREMGFSIAATTGTARYLFENGILPEVVLKVHEGSPNVVDHMRAGRIQM
                     LINTPLGRHSLHGDKEMRIEAVRRKIPYTTTTSAAEAAVEGIAALKEGTLRIRRL"
BASE COUNT          271 a          455 c          384 g          279 t
ORIGIN      
        1 cctgatcaac cagttcctct ctccgttgag caaccgcaga cgtgacggct acggcgtaaa
       61 cttcgagggc cggtcccgct ttctccagga gatcgcgagg gcggtacgga cgcgctggcc
      121 cgaggagaaa ccgctcttcc tccggatttc ggcggaggac tacgtcgagg gaggtatgac
      181 cacaggacag atggtccgta tcgtcgacag agtcaccaag gaagtggacg tcgtccacgt
      241 cagctccgga ggggtaaccc cggtggctcc ccctgtgcat ccgggatatc agattccctt
      301 cgccgagcag atcaagaggg agtgcgaggt tccgaccatc gcggtcgggc tgatcaccag
      361 tctcgacatg attgaggagg cgctttgcaa tcaaagggcc gacctcgtcg ccctggggcg
      421 cgagctgctc cgtaacccct tcttcccgct gaacgaggca agaaaacgcg gcatagagct
      481 cccctggcct gagcaataca gaagagcgtt cagctaagaa ccgcgagggg cttcctctat
      541 tcggaagccc ctcgattcat tatttcccta cagcctccgt atgcggagcg taccctcttt
      601 cagcgccgcg atcccctcca cggccgcctc cgccgccgac gtcgtggtag tgtacggtat
      661 cttccgtcgg actgcttcga ttcgcatttc cttgtctccg tgcaggctgt gccgcccaag
      721 aggggtattg atgagcatct gaattctccc ggcacgcatg tgatctacta cgttgggtga
      781 gccttcatga accttcagca ccacttcggg aagaatgcca ttttcaaaga gataacgcgc
      841 tgttcctgtt gtagccgcta tcgaaaaccc catttcacga agtcgcttcg cgaccgggag
      901 aattgtgggc ttatccgggt cgttcaccga aatgaacacg gttcctttcg tcggcagcac
      961 cgtcccgcag gcggcttgcg ccttcgcgta ggcttccccg acgctctctc cagatcccat
     1021 gacctctccg gtggagcgca tttccggccc gagcaggggg tccaccgatg cgaacctgtc
     1081 gaacgagaag acggcctctt tcaccgccca ccccgtaagg caccgcccct ctccgtaacc
     1141 gttccggaca agcccctgcg cttcgaggtc cttcccctcc caaagacgga cggccgccct
     1201 cacgagatcg acgccgctcg ccttcgacag gaaggggacc gttctggaag ctctgggatt
     1261 cacctccagc acatagagca ccccctcctt catggcaaac tgaatgttga gaaaccctct
     1321 cacacctatc gcgcgcgcga tggaaacggc ggcggcgacc atctcactga ggatttccgg
     1381 aggcgactt
//
LOCUS       DAOPKU010000712         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_113316_length_1389_cov_2.365817, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000712 DAOPKU010000000
VERSION     DAOPKU010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113316_length_1389_cov_2.365817"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1389)
                     /locus_tag="PK849_15180"
     CDS             complement(<1..>1389)
                     /locus_tag="PK849_15180"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPE67525.1"
                     /translation="LSYTGFFAREDLFEVHKEGGKADELDVAYFVPRSQLGEYAEGER
                     AKDGEGNLKCFPKGTFIYRLAGRERQKSASYYTPESLVKCLVKYALKELLEGKKADEL
                     LETTVCEPAMGSAAFLNEAADQLADAYLQRKQRETGKEIPLDEYTYEKQRVKMYLAAK
                     GVFGVDLNPVAVELAEVSLWLGAIHRDAPVPWFGMQLVCGNSLVGARRAVYDSELLRP
                     AVQGAPSWKDGPPETLKNFKRRKGTVYHFLLPSESMAEYGDKTVKSLLPDEVKRVKEW
                     RKHFCKPFTEGEINRLESLSEAVDGLWSACAEEQARLREEMADSLPVFGRPAHRKAAA
                     TSRHKDLRYAQEILSRRVRASSPYRRLKLAMDYWCALWFWPLEKADMLPSREEWLFDM
                     ELLLHGGVIDSVRTGESQLPLFPSTAKEEAEQLVLRFGMVNVETLLSGNGRLKTADEL
                     ARKYRFLHWEIE"
BASE COUNT          214 a          463 c          441 g          271 t
ORIGIN      
        1 actcgatctc ccagtgcaga aagcggtatt tccgcgccag ctcgtcggcg gttttcagcc
       61 gcccgttccc cgagaggagc gtctccacgt tgaccatgcc gaagcgcagg acgagctgct
      121 ccgcctcctc cttcgcggtg gaggggaaga gggggagctg gctttcgccc gtccgcacgc
      181 tgtcgatcac ccctccgtgc agcagcagct ccatgtcgaa gagccactcc tcgcgggagg
      241 ggagcatgtc cgccttctca aggggccaga accagagggc gcaccagtag tccatggcca
      301 gcttcagccg ccggtagggg ctggacgccc gcacccggcg ggagaggatc tcctgagcgt
      361 accggaggtc cttgtgccgc gaggtcgccg ccgccttccg gtgcgcggga cgtccgaaga
      421 ccggcagcga gtcggccatc tcctcccgga ggcgcgcctg ctcctccgcg caggcggacc
      481 acagcccgtc gaccgcttcg gaaagggatt cgagacggtt gatttcgcct tcggtgaacg
      541 gcttgcagaa gtgcttccgc cactccttca ccctcttcac ctcgtcgggc agcaggctct
      601 tgacggtctt gtccccgtac tccgccatgc tttccgaggg gaggaggaag tgatacaccg
      661 tccccttccg ccttttgaag tttttcagcg tctccggagg gccgtctttc catgatggcg
      721 ctccctgtac tgcgggcctg aggagttcgg aatcatagac ggcgcgccgc gcgccgacga
      781 gggagttgcc gcagacgagc tgcatcccga accacgggac gggagcgtcc cggtggatgg
      841 cccccagcca gagagagacc tcggcaagct cgaccgcgac ggggttgagg tcgacgccga
      901 agacgccctt cgccgcgagg tacatcttga cccgctgctt ttcgtacgtg tattcgtcga
      961 gggggatttc cttgcccgtc tcccgctgtt tccgctgcag gtaggcgtcc gcgagctggt
     1021 cggcggcttc gttgaggaag gcggcgctgc ccatggcggg ctcgcagacg gttgtttcaa
     1081 gcagttcgtc cgcctttttc ccttccagca gctccttgag ggcgtacttg acaaggcatt
     1141 tgaccagcga ttccggcgtg tagtacgagg cgctcttctg ccgctccctc ccggcgaggc
     1201 ggtagatgaa ggttcccttc gggaagcact tcaggttccc ctccccgtcc ttcgcccgtt
     1261 ccccctccgc gtactctccg agctgcgagc gggggacgaa gtaggcaacg tccagttcgt
     1321 cggccttgcc gccctcttta tggacctcga agagatcctc cctggcgaag aagcccgtgt
     1381 aggagagaa
//
LOCUS       DAOPKU010000713         1382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_114116_length_1382_cov_2.968350, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000713 DAOPKU010000000
VERSION     DAOPKU010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114116_length_1382_cov_2.968350"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PK849_15185"
     CDS             <1..517
                     /locus_tag="PK849_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67526.1"
                     /translation="KQNAERNQAEESGRDWARERDKWLENVESFYTTIFSYLREYIDR
                     GQIVVQQAPVTLFEEDVGEYSTKTATLSLGKKQIKFEPIGTNLIGAKGRIDMIGPNGK
                     VKFVLVDKAASSPEFVVEISVDGEAPSKKAQEDVRREWVWKISTPPPRIGYFELNEES
                     FFDAMMEVANA"
     gene            510..1055
                     /locus_tag="PK849_15190"
     CDS             510..1055
                     /locus_tag="PK849_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67527.1"
                     /translation="MLERVSFSGQHLELSRIVSHHTDVESALKLYFSPSSPDYVVRFA
                     DYVASEVGNELLDRLDEHERWTAFSILSAVEAAFRIDYLQRNYKKKKDPLSRELRALH
                     KRKGSRASLEDDIFDAWKKRTSVPGRLIEDLKGAFKYRHWLAHGRYWEPKLGRRYDYD
                     GVYILAKTVFSSFPLEGAEPH"
     gene            complement(1115..>1382)
                     /locus_tag="PK849_15195"
     CDS             complement(1115..>1382)
                     /locus_tag="PK849_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67528.1"
                     /translation="EKELSPPYFPSSGQVILLHIVVAVSRMVISTQSRPAISVLLRNP
                     PEMIPALGEFLHTFSFCGFLCCERQNNSSRESSRDSSRSSQLGR"
BASE COUNT          357 a          345 c          385 g          295 t
ORIGIN      
        1 aaaacagaat gccgaacgca atcaggcgga agaatcggga agagactggg cacgggagcg
       61 cgacaaatgg cttgaaaacg tcgagtcctt ttatacgacc attttttcct accttcggga
      121 atacatcgac cgtggacaga tagtcgttca gcaggccccc gttacgcttt tcgaagagga
      181 cgtcggcgaa tactcgacga agacggcgac gctctccctt ggcaaaaaac agatcaagtt
      241 cgagcctatc ggtaccaatc tgattggagc caaagggcgt atcgatatga tcggccccaa
      301 cggaaaggtg aagttcgtcc tcgtggacaa agccgcgtct tcgccggagt tcgttgttga
      361 aatctccgtg gacggagagg ccccctcgaa aaaggcgcaa gaggacgtgc gccgcgagtg
      421 ggtctggaag atcagcaccc cgcctccgcg cataggatac ttcgaactga acgaggagtc
      481 cttcttcgat gcgatgatgg aagtcgcaaa tgcttgaaag ggtttcgttt tcgggacagc
      541 atcttgagct gtcccggatc gtatcgcatc ataccgatgt ggaatcggct ctcaaactgt
      601 atttcagccc ctcttccccc gactatgttg tccgtttcgc cgattacgtt gcatcggaag
      661 tcgggaacga gcttctcgac cgtctcgacg agcatgaacg atggacggct ttttccattc
      721 tatcggctgt ggaggctgcc tttcgtattg actatcttca gcgcaactac aaaaagaaaa
      781 aggatcccct ttcgagagag cttcgcgcgc tccataaaag aaaaggctca agagcgtctt
      841 tggaagacga tattttcgac gcctggaaaa agcgcacgtc cgttcccggc aggttgatag
      901 aggatctgaa aggcgccttc aaataccggc actggcttgc ccacggccgg tactgggagc
      961 cgaagctggg ccggcgatac gactacgacg gagtgtatat tctggcgaaa acggtctttt
     1021 cctccttccc gctggagggt gcggaaccgc attgaggcga tgtggttgca gacctttcga
     1081 agaaaacagg ccccagtcaa aagtcagacg agactcaccg accaagttgt gatgaacggc
     1141 tagagtcacg gctagactca cggctagagt tgttctggcg ttcgcagcat agaaagccgc
     1201 aaaaagagaa tgtgtgaagg aactctccca atgctggaat catttcggga ggattgcgca
     1261 acagaaccga aattgccggt cttgactggg ttgaaataac cattcgtgaa acggcaacga
     1321 caatatgcag aagtatcacc tgaccggaag aggggaaata aggtggcgaa agctcttttt
     1381 ca
//
LOCUS       DAOPKU010000714         1374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_115085_length_1374_cov_3.834723, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000714 DAOPKU010000000
VERSION     DAOPKU010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115085_length_1374_cov_3.834723"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK849_15200"
     CDS             complement(<1..183)
                     /locus_tag="PK849_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPE67529.1"
                     /translation="MAGTLSSLYVGGMVWTFFASAVALISLAEFYGMLSRKFKVSKGI
                     GFLTSGLIIFSVSEGIH"
     gene            complement(230..928)
                     /locus_tag="PK849_15205"
     CDS             complement(230..928)
                     /locus_tag="PK849_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPE67530.1"
                     /translation="MIPKETVPRHVAIIMDGNGRWAKKRFLPRLLGHKAGVKAIERTV
                     RAADALGIQYVSLYAFSTENWKRPEKEVSGLMSIFRFVLRRKVDEMKRENVRLRFSGF
                     RDGMPQDIVELMEWAEEETKENSGLTLVPCLNYGGRQEILHAVENLLEDGVRPPLTEE
                     RFREYLYLPDLPDPDLIVRTSGESRLSNFWLWQSTYSELYFTDTLWPDFDGKELENAV
                     KYFANRERRYGALK"
     gene            complement(925..>1374)
                     /locus_tag="PK849_15210"
     CDS             complement(925..>1374)
                     /locus_tag="PK849_15210"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPE67531.1"
                     /translation="DEDEQGVPFVGRAGKLLTRILQSVGIERESVFITNVVKCRPPQN
                     RTPMADEMMACTPFLEAQLALLRPKIVVCLGNTPAKWLLKSSEGISALRGRWFAWRGI
                     DLFPMFHPSYLLRNESKKKGSPKDLTWQDINALRSKLDDLTKGGPSA"
BASE COUNT          334 a          388 c          323 g          329 t
ORIGIN      
        1 gtgaatcccc tctgatactg aaaagataat aagccccgag gtaaggaagc ctattccctt
       61 ggagactttg aacttcctgg aaagcatccc gtagaattcg gcgagagaga tcaaagcaac
      121 agcggacgca aaaaatgtcc acaccatacc gccgacatag agggaactga gcgtacctgc
      181 cacgatgacg agagaacttc cccctcgcag agtaagttcc cgtagctttt cacttgagag
      241 cgccatagcg tctctctcta ttcgcaaaat atttcacggc gttttcaagt tcctttccgt
      301 caaaatccgg ccacaaggtg tccgtaaaat acagctcgct atacgtgctc tgccagagcc
      361 agaagttact caaccgactc tcgccgcttg tccggacaat gagatcaggg tccggaagat
      421 ccggaagata gaggtattct ctgaaacgct cctcggtcaa aggagggcgg acgccatctt
      481 ccaggagatt ctccactgcg tgcagaatct cctggcggcc accgtaattg agacagggaa
      541 cgagcgtgag accgctattc tcctttgttt cttcttcggc ccactccatc agttcaacta
      601 tatcctgggg catcccgtca cgaaaaccgg agaagcgaag acgcacattc tcacgcttca
      661 tttcatcaac ttttcggcgc aagacaaaac ggaagatgct cataagacct gagacctctt
      721 tttcaggcct tttccagttt tctgttgaaa aagcatacag agaaacatat tgaataccaa
      781 gcgcgtcggc tgcccgtacc gtccgttcaa tagccttcac tcctgcttta tgccccagca
      841 gacgcggaag aaagcgtttt tttgcccaac gcccgttgcc gtccattatt atggcgacgt
      901 ggcgaggtac tgtctccttc ggtatcatgc ggacggccct cctttcgtca gatcatcgag
      961 tttgctgcgg agagcgttga tatcctgcca tgtcaaatcc ttcggactcc ccttcttttt
     1021 tgattcgttg cggagaagat agcttggatg aaacatcggg aaaagatcga ttcccctcca
     1081 tgcaaaccaa cgtccgcgaa gtgcggaaat gccttctgag gacttgagca gccattttgc
     1141 cggggtgttt cccaggcaca ccacgatctt cggccggaga agggccagct gcgcctccag
     1201 aaatggcgtg catgccatca tttcgtccgc catcggagtg cggttctgcg ggggccggca
     1261 ctttacaaca ttcgtaataa aaacgctttc cctctcaata ccgaccgatt gaagaatacg
     1321 cgtgagcagt tttcccgctc gtcctacaaa tgggactcct tgttcatcct cgtc
//
LOCUS       DAOPKU010000715         1372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_115307_length_1372_cov_3.790433, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000715 DAOPKU010000000
VERSION     DAOPKU010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115307_length_1372_cov_3.790433"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..>1372
                     /locus_tag="PK849_15215"
     CDS             251..>1372
                     /locus_tag="PK849_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE67532.1"
                     /translation="MNNRMKNGNPAPVLDDVVLGAVESLSQQLQQMFWVGKNQEKAAE
                     EMRTSASSTRSDMERYALGVSKIIEGLSHLSESCIAMGDIAMASKEIESETREGVLNA
                     VTGMERLEKTLDALSTLSEEHARMTDELLHSTADVGIFLERIDRIAKQTRLLALNASI
                     EAARAGEAGRGFNVVAQEVGKLAQSTQETAASISKAIETMGDRIRHVVDGLKETESAL
                     SAGREETQDALARARQAGEGVMRFSGSFSELIKVLEEERTSITEAVAETQSLQEGTKA
                     VLSDVGTLGKSLEAEVSMAKEFSASIKMVAEDLFTLQQSVSSQRPSDELWIGLTPFTD
                     PQKIRDKFGPLLAEVARRMGRKSRLFVSPDYESLGKMLAT"
BASE COUNT          311 a          346 c          422 g          293 t
ORIGIN      
        1 tcggggtgtt tgcgggttca gctctctggt ggcttctttt gagcggcggc ttcggcctgt
       61 tccgggagaa attcaccgcc agcggtctca gatggctcaa tagactttcc ggggtaatca
      121 tcggtgtttt tggtctcctc gcactgtgga gcgttttcta gattcataat gtccacatga
      181 cgaaaagagg gaacccgtca aaagaagcag cgcgcgttta gtaccggcct cagaaaggag
      241 tgttgttcgt gtgaacaatc gtatgaagaa cgggaatccc gcccccgtgt tggacgatgt
      301 cgttctcggc gcagtagagt ccctttcgca gcagctgcag cagatgtttt gggtaggcaa
      361 aaaccaggaa aaagcagcgg aagagatgcg gacgagcgcg tcctctaccc ggtcggacat
      421 ggaacgatac gccttggggg tctccaagat catcgaaggg ctttcgcatc tttcagaatc
      481 ctgtattgca atgggcgata ttgccatggc ctcgaaagag atcgagtccg agacgcgcga
      541 gggagtgctc aatgctgtga cgggtatgga gcgcctggag aaaacgttgg acgctctttc
      601 cactctctct gaggagcatg cgcgcatgac cgatgagctt ttgcactcga ctgcggatgt
      661 cggaatcttt ctggagcgaa tcgaccgtat tgcgaaacag acgcggctcc tcgcgctcaa
      721 tgcgtccatt gaagctgcaa gggccgggga ggcggggcgc ggtttcaacg tcgtcgccca
      781 agaggtgggg aaattggcgc agtccacgca ggagacggcg gcgtccatct caaaagctat
      841 cgaaacgatg ggagaccgca tccggcatgt cgtcgacggc ctgaaagaaa cggagagcgc
      901 gctctccgcc ggaagggaag aaacgcagga cgccttggcg cgcgctcgtc aggcgggcga
      961 aggcgtcatg cgtttcagcg gttcgttctc cgagctgatc aaagttctcg aagaggaacg
     1021 tacctccatc acggaggctg tagcggaaac acaatctctg caagaaggga cgaaggcagt
     1081 cctttccgat gtaggcactc ttgggaaaag ccttgaagcc gaggtttcga tggcaaaaga
     1141 gttttcggct tcgatcaaga tggtcgccga agatctcttt acgctacagc aaagcgtctc
     1201 gtcccagcgt ccttcagatg aactgtggat tggactgact ccgtttaccg acccgcagaa
     1261 gatacgggac aaattcggcc ccctgcttgc cgaggttgca aggcggatgg gaaggaaaag
     1321 ccgtcttttc gtctctcctg attatgagag cctcggaaaa atgcttgcga ca
//
LOCUS       DAOPKU010000716         1372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_115385_length_1372_cov_1.900532, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000716 DAOPKU010000000
VERSION     DAOPKU010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115385_length_1372_cov_1.900532"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1372
                     /locus_tag="PK849_15220"
     CDS             <1..>1372
                     /locus_tag="PK849_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67533.1"
                     /translation="KLCSSCMALSDVRDGEDCPFCGNAPLLPVFVPDSQFKKTYENGD
                     NRVFSHNDCPFCGSSRGLSIFGARSTTLSSVMISQTMASRGNDDKKMLAFSDSVQDAA
                     HHAGFFGARTYRFTLRTAMAKYLADGGEGKTLSGFAGELPRYWASRLSMEECVALFLA
                     PDQAWRRDYARLKETGAPPGAEFMEQLSKRIGWEAFTEFGLSAHIGRTLEKSGVAVAS
                     PDPALFAHSEALLAEALKNELPKMTPPEEREISFFLSGLIQRMKSKGAIHHPELEEYI
                     RRGGEGYLLSKERKGWMPSYGKRTRRPLFLSAAADGDFDRLSFSESRNWYCRWMMKCF
                     GENNPMMLSSAADFWRTVLGVLSSEPSGILRRLSWPRGRGSEDPVWALEPSALRMTCG
                     VAEFRCSKCGASSFAGADGAEEWNGLPCLRDSCDGRLAESSISGEYYGRLYRTGDMCR
                     LFPAEHT"
BASE COUNT          235 a          421 c          460 g          256 t
ORIGIN      
        1 gaaagctctg ttcctcgtgc atggcgctct ccgacgtgcg ggacggagag gactgcccct
       61 tctgcggaaa cgcccccctg ctcccagtct tcgttcccga ctcacagttc aagaagacct
      121 acgagaacgg cgacaacagg gtattctcac ataacgactg ccctttctgc ggttcgtcaa
      181 gggggctctc catcttcggc gcccggagca cgaccctgtc gagcgtcatg atctcgcaga
      241 ccatggcgtc gcggggaaac gacgacaaga agatgctggc cttctccgac tcggtgcagg
      301 atgccgccca tcacgccggg ttcttcgggg cgaggaccta caggttcacg ctgcggaccg
      361 ccatggcgaa gtaccttgcc gacggcgggg aggggaagac gctctccggt ttcgccgggg
      421 agctgccccg gtactgggct tcccgcctgt ccatggagga gtgcgtcgcg ctttttctcg
      481 ccccggatca ggcgtggcga agagattacg cccgcctgaa ggagacgggt gcgccgccgg
      541 gcgcggagtt catggagcag ctgtcgaagc ggatcgggtg ggaggcgttc accgagttcg
      601 gcctgagcgc ccacatcggg cgcaccctgg agaaatcggg cgtggccgtc gcctcgcccg
      661 acccggcgtt gttcgcccat tccgaagccc tcctcgcgga ggcgctgaaa aacgaactgc
      721 cgaagatgac gccgccggag gagcgggaga tctctttctt tctctccggg ctgatccaga
      781 ggatgaagtc gaagggggcg atccatcacc ccgagctgga ggagtatatc cggcgcggag
      841 gagaggggta tctgctctcg aaagagcgga aagggtggat gccctcctac gggaagcgga
      901 cgcggcggcc tcttttcctc tccgcggcgg cggacggcga tttcgaccgc ctctcctttt
      961 cggagtcgag aaactggtac tgccgttgga tgatgaaatg cttcggggag aacaacccca
     1021 tgatgctctc gtcggcggcg gatttctggc ggacggttct cggcgttctc tcgtccgaac
     1081 cttccggcat cctgaggagg ctttcctggc ccagaggaag ggggagcgaa gatcctgtat
     1141 gggctcttga accttccgcg ctccgcatga cttgcggcgt ggccgagttc cggtgctcga
     1201 agtgcggcgc gtcctctttt gcgggggcgg acggcgcgga ggaatggaac ggccttccct
     1261 gcctccggga ctcctgcgac ggcagactcg ctgaaagctc catatcgggg gagtactacg
     1321 gaaggctgta ccgcacggga gacatgtgcc gccttttccc cgcggagcac ac
//
LOCUS       DAOPKU010000717         1366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_116038_length_1366_cov_3.712433, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000717 DAOPKU010000000
VERSION     DAOPKU010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116038_length_1366_cov_3.712433"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PK849_15225"
     CDS             <1..655
                     /locus_tag="PK849_15225"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HPE67534.1"
                     /translation="LPGPVFRAAKEETVPFPKVRELVDRIRSAGIEILLCDNGTDVRV
                     PTWNCFLFDMEDAGGIGGVAHGMGSSLDVESAMIRAVTEAVQGRCVFLSGVRDIVPSK
                     EFGGFMSGDARLAKEAMESAVGEWLDMSGVKDAPFATFEEDVRETVRRLSAVGLDRVI
                     VYRLSRPDDPVQAAKVVVPGAEGYIFPYYLPGERAVAAIRHGLGEGSPLFREEGFSW"
     gene            649..>1366
                     /locus_tag="PK849_15230"
     CDS             649..>1366
                     /locus_tag="PK849_15230"
                     /inference="COORDINATES: protein motif:HMM:NF019429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HPE67535.1"
                     /translation="MVAIIFGGPSLEGVDKTKTDAIFLPAAAQGDLLRAVREHSPSVI
                     GLLDCRLPYQSLPTWHKEILFALARGVAVVGAAAVGAHRAVELGPNGMEGVGGIYERV
                     VSGELERDDEVLCDWEETPDGIRRLTLPLVTMRDGVAAAKNAGTLSDESASSLLSLAE
                     RLFWKKRTWRALLDAAAAEGVPEEELRAFEAWLPAAPDPVKEDAEALLRRVEELASGN
                     GESGRTFQNDSAGKGDPGEES"
BASE COUNT          229 a          432 c          489 g          216 t
ORIGIN      
        1 gctcccaggc ccggtcttcc gcgccgccaa ggaggagacg gttcccttcc cgaaggtgcg
       61 ggaactggtc gaccggatac gctccgccgg catagaaatc ctgttgtgcg acaacgggac
      121 ggacgtccgc gtcccgacct ggaactgctt cctcttcgac atggaggacg cgggcgggat
      181 aggcggggtg gcccacggaa tgggaagctc tctcgatgtg gagagcgcga tgatccgcgc
      241 cgtgaccgag gcagttcagg ggcggtgcgt cttcctctcc ggcgtgcgcg acatcgttcc
      301 gtcgaaggag ttcggcggct tcatgagcgg cgacgcgcgc ctcgcgaagg aggcgatgga
      361 gagcgccgtc ggcgaatggc tcgacatgag cggcgtgaag gacgcgccct tcgccacgtt
      421 cgaggaggac gttcgcgaaa cggtgcggcg gctctcggcc gtcgggctcg accgggtgat
      481 cgtctacagg ctttcccgcc ccgacgatcc tgtgcaggcg gcgaaggtgg tcgttcccgg
      541 cgccgaaggg tacatcttcc cgtactacct gccgggtgag cgcgccgtcg cggccatcag
      601 gcacggactc ggagagggaa gccccctctt ccgcgaggag gggttctcat ggtagcgatc
      661 atcttcggcg gtccctccct ggagggcgtc gacaaaacga aaacggacgc gatctttttg
      721 cccgccgccg cgcagggcga ccttctgcgg gcagtgcgcg aacacagtcc gtcggtgata
      781 ggactgctcg attgcagact cccctaccaa agcttgccga cgtggcacaa agagatcctc
      841 ttcgccctcg cgcgcggggt ggcggtcgtc ggcgccgccg cggtcggagc gcaccgcgcc
      901 gtcgaactgg ggcccaacgg catggaaggg gtcggaggca tctatgagcg cgtggtatca
      961 ggagagctgg agcgcgacga cgaagtgctc tgcgactggg aagagacgcc ggatggaatt
     1021 cgccgcctca ccctgccgct ggtgaccatg cgcgacggcg tggccgccgc gaagaacgca
     1081 ggaactctct ccgacgaaag cgcttcgtcg ctgctctcgc tcgccgaacg gctcttctgg
     1141 aaaaaacgca cttggagggc gctcctcgac gctgccgctg cggagggcgt gcccgaggag
     1201 gagctgcgcg ccttcgaggc gtggctcccc gccgcccccg accctgtgaa ggaggacgcc
     1261 gaagcgctgc tgcgccgcgt ggaggaactc gcctccggga acggggagtc cggaaggact
     1321 ttccagaatg attccgcggg aaaaggtgat cccggcgagg agtccg
//
LOCUS       DAOPKU010000718         1354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_117642_length_1354_cov_3.301771, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000718 DAOPKU010000000
VERSION     DAOPKU010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117642_length_1354_cov_3.301771"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK849_15235"
     CDS             complement(<1..220)
                     /locus_tag="PK849_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE67536.1"
                     /translation="MTESKIRVLVVDDSSFMRKVISDILGADPRMEVVGTARDGEDAL
                     RKIEELSPAVVTLDVEMPKKDGLATLEEI"
     gene            complement(238..909)
                     /locus_tag="PK849_15240"
     CDS             complement(238..909)
                     /locus_tag="PK849_15240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake domain-containing protein"
                     /protein_id="HPE67537.1"
                     /translation="MDSGEFLAMLKDCIGSKVSLVIDAGLYKGTYPSRVEDVDEEAIG
                     LAHPMMRGALLPVHRNVLLKMSVEAGGALYFSVVSISKGLVRDTLPLLWVAPVGEARK
                     IQRRLFVRVPCLMKIGFFPLEGENYKPEKEEWVYVNAKDISLGGVGFTVPHEKERFFP
                     EKTRFLVEIVLQEKKYLLVGKLVKKMPKNELLELGMAFEGVPGFVEKPLGGYIRQQEL
                     AGRQS"
     gene            complement(912..>1354)
                     /locus_tag="PK849_15245"
     CDS             complement(912..>1354)
                     /locus_tag="PK849_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPE67538.1"
                     /translation="TLSFGVAADMTILITTPEPTSIRDAYGVLKTLAINTGSKIDVTL
                     IVNMAASRSEGREVAERIQMAANQFLSLSVPYGGVVLLDENVGKAVRMRTPFFLANPS
                     CQASKDIAEIAANLASIDDETEPPTGRGVKSFFFRLARSLGLGV"
BASE COUNT          304 a          422 c          311 g          317 t
ORIGIN      
        1 tgatttcctc aagagtagcc aaaccgtctt ttttgggcat ctcgacatca agcgtaacaa
       61 ccgccggaga aagctcttca atcttgcgaa gagcgtcttc tccgtcccga gctgtcccga
      121 caacctccat ccggggatcc gctcccagta tgtcgctgat tacttttctc ataaaagagg
      181 agtcatcaac tacaagcact cgaatcttcg actctgtcat agcattccct tcgctctcta
      241 tgattggcgt cccgccagct cctgctgacg aatgtagcct ccaagcggct tctccacaaa
      301 acccggcacc ccttcaaagg ccattcccag ctccaacaat tcatttttgg gcattttttt
      361 tactaatttg ccaaccagca gatatttttt ttcctgaagc acaatctcca ccaggaaacg
      421 cgtcttttcg gggaaaaagc gctctttctc atgcggaaca gtaaaaccta ccccccccag
      481 gctgatatcc ttcgcgttga catacaccca ctcttctttt tcgggtttgt agttctcgcc
      541 ctcaagagga aaaaaaccta ttttcataag acatggaacg cggacaaata gccgccgttg
      601 aatcttgcgt gcctcgccga ccggcgctac ccagagaagc ggcagggtgt cgcgtaccag
      661 ccccttcgag atggacacca ccgaaaaata cagggctcct cccgcctcca cgctcatttt
      721 cagcagcacg ttgcgatgca cgggcaacag cgcgccccgc atcatcggat gggcaaggcc
      781 tattgcctcc tcgtcgacgt cctctactct tgaagggtac gtccccttgt agagtcccgc
      841 gtcgatgaca agcgacacct tgctcccgat gcagtccttg agcattgcga gaaattcgcc
      901 ggagtccatt tttacactcc caacccgaga cttctggcaa ggcggaagaa gaaggatttt
      961 acacctcttc cggtcggagg ctcagtctcg tcgtctatgc tcgccagatt cgccgcgatc
     1021 tccgcgatat ccttactggc ctggcacgac ggattcgcca ggaaaaaagg agtacgcatc
     1081 cggaccgcct ttccgacgtt ctcatccaac agcaccactc cgccgtaagg aaccgagagg
     1141 ctgagaaact ggttcgccgc catctgtatg cgttcggcga cctctcttcc ctcggaacgc
     1201 gaggccgcca tattcacgat gagcgtcacg tcgattttgc tgccggtatt gatagccagc
     1261 gtcttcagga caccgtacgc gtcgcgtatg gatgtcggct caggggttgt gatcaagatc
     1321 gtcatatccg cagcaacgcc gaaggagagc gtat
//
LOCUS       DAOPKU010000719         1343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_119116_length_1343_cov_3.431677, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000719 DAOPKU010000000
VERSION     DAOPKU010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119116_length_1343_cov_3.431677"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PK849_15250"
     CDS             <1..211
                     /locus_tag="PK849_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67539.1"
                     /translation="RILGIDSKGHQQIVKAQAPLAEMFRYAIILRSMTSGRGSFTMEY
                     DHYEEVPAEITKKVVAAHKQEEEEE"
     gene            301..>1343
                     /locus_tag="PK849_15255"
     CDS             301..>1343
                     /locus_tag="PK849_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPE67540.1"
                     /translation="MKKRVIVVGSGGAGLTAAISARKTGAEVVLLSKTGCAEASCTAY
                     SGGLFSLSSGTVSPDDHYRRIMETGRYVNDPSLVRILADHSEATLRTISEWGVSLKVT
                     TSGHATARKTAPSRIMGGGGMTRELASIARREGVVILENHIASRLLVGTHGVEGVEAV
                     DWKRGKAFSFGASAVILATGGGGAIYERTDNPLRMTGDGYALALDAGLELVDMEYVQF
                     YPMGWDQKGYPVWMVGLGLLDYIPVTDENGREFLKEAFPLWGVSTGKEANLFARDRAA
                     LLLAGHAVKGGRTLLHLEKFREEDLSLPDVQASLLFDLPPEKRSAPVHVSPVQHYFTG
                     GVPMDSEGRTSIPG"
BASE COUNT          301 a          345 c          407 g          290 t
ORIGIN      
        1 acgtatcctc ggcatcgaca gcaagggaca ccagcagatc gttaaagcgc aggcgcctct
       61 tgcggagatg ttccgctatg cgattatcct tcgctcaatg acctccggaa gaggctcctt
      121 cactatggag tacgatcact acgaagaagt cccggccgag attacgaaaa aggttgtcgc
      181 tgcacacaag caggaagagg aagaagaata agctttttct caaacacgag ttctcaagag
      241 agccccgcgc gatgcggggc tctcttgtaa taagcacgtt tcacaaggag gccggataca
      301 atgaaaaaaa gagtgattgt cgtcggttcg ggaggagccg gtctgactgc tgctatctcc
      361 gcccgcaaga ccggcgcgga agttgtgctt ctcagcaaaa cggggtgtgc ggaggccagt
      421 tgcacggcct attccggcgg actcttctct ctttcgtcgg ggaccgtttc accggatgat
      481 cactaccgtc ggataatgga aacgggacgg tacgtcaatg atccatcgtt ggttcgaatc
      541 cttgcggatc attccgaagc cacgctcagg acaatctccg agtggggagt ctccttgaaa
      601 gtcactacat ccggtcatgc gactgcacgc aagaccgccc cttcgagaat catgggcggc
      661 ggagggatga cccgtgagct tgcctcgatc gcgagacgcg aaggtgtcgt cattctggag
      721 aatcatatag cttcacgtct gttggtcggt acgcatggcg ttgagggagt ggaagctgtc
      781 gactggaagc gagggaaggc gttctctttc ggcgcatccg ctgtgattct tgcgaccgga
      841 ggaggcgggg cgatctatga acggacggac aacccgctgc gtatgacggg agacggatac
      901 gcgctcgcac ttgatgccgg tcttgaactt gtggatatgg agtacgtgca gttctacccg
      961 atggggtggg atcagaaagg ataccccgtc tggatggtcg gactggggct gcttgactac
     1021 attccggtta cggatgagaa cggaagagaa tttctcaagg aggccttccc tttatggggc
     1081 gtctccaccg gaaaagaagc gaatctcttt gcccgcgacc gtgcagcgct cttacttgcg
     1141 ggacatgctg tgaaaggggg aaggacgctg cttcatctcg agaagttcag ggaagaagac
     1201 ctctctctcc ccgatgtcca ggcttcgctg ttgttcgacc ttccgccgga gaaacggtcc
     1261 gctccagtgc acgtcagtcc ggtacagcac tattttacag ggggcgttcc aatggattcc
     1321 gaagggagaa cctcgattcc agg
//
LOCUS       DAOPKU010000720         1341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_119355_length_1341_cov_4.905132, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000720 DAOPKU010000000
VERSION     DAOPKU010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119355_length_1341_cov_4.905132"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PK849_15260"
     CDS             complement(<1..403)
                     /locus_tag="PK849_15260"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67541.1"
                     /translation="MIRKSTACGVLGAFVLGLLLTGVFALPSAAADKLVLKVAGISSP
                     EYRGSKSLEAIKEIVEKGTEGRVVLDVFPANQLGDYTQVFEEIRRGTIEMGLIFLPSQ
                     FDPMLEVGSLPFLASTGEGSEPTSSMGSNWLG"
     gene            complement(493..1245)
                     /locus_tag="PK849_15265"
     CDS             complement(493..1245)
                     /locus_tag="PK849_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPE67542.1"
                     /translation="MSSLEKALLLLKRLGEPPYELGLSEIAVHAGIGKSGAYKLLRTM
                     QEQNFVIQDHSSKKYHLSPIMLRLGNVYGHFKGIGETAPPILAHTSKTVGETTYITIW
                     EGDRAFPAYKHRVPGGVYDVNDFIGKSIPLNSGGSAMLLCAYQDPEHIRSLLDASELE
                     RRTPYTLVDRDVLMEEYRKIRDQGYALEDEVFNIGVLSLAVPVFDRERAVRISLAMAA
                     PKKHATEGKIVEWLHLLKDGAEELSYKLQFRR"
BASE COUNT          276 a          406 c          359 g          300 t
ORIGIN      
        1 tccccagcca gttcgacccc atgctcgaag tcggctcgct cccctcgccc gtggaggcaa
       61 ggaaggggag cgagccgact tcgagcatgg ggtcgaactg gctggggagg aagatgagcc
      121 ccatctcgat cgtgccgcgc cggatctcct cgaaaacctg cgtatagtcg ccaagctggt
      181 tcgcggggaa gacgtccaga accacccgtc cctccgtccc cttctcgaca atctccttga
      241 tggcttcgag cgacttgctt ccgcggtact ccggagagga gatcccagcc accttcagca
      301 caagtttgtc cgccgcggcg gacggcagcg cgaaaacccc cgtcagcagc aacccgagaa
      361 caaacgctcc cagaactccg cacgcagtac ttttccttat cattatgaca tcgctccttt
      421 cagattcttt agtgccttgt acagaccttt tccgcgcagt catttttccc cgcaactcct
      481 cctttcacgg cgtcaacgcc tgaactggag cttgtacgaa agttcctcgg cgccgtcttt
      541 cagaagatga agccattcga cgattttccc ctccgtcgcg tgcttcttgg gcgcagccat
      601 ggcgagagag atgcgaaccg ctctctccct gtcgaaaacc ggaactgcga gtgaaagcac
      661 cccgatattg aagacctcgt cctccagggc atacccctga tcgcgaatct tccggtactc
      721 ctccatcaaa acgtctctgt caacaagcgt gtagggtgtg cggcgctcca gctccgaagc
      781 gtcaagaagg ctgcggatat gctccggatc ctggtaggcg cacagcagca tcgcggaccc
      841 tccggagttc agcggaatac tcttgccgat gaaatcgttc acgtcgtaca cgcctccggg
      901 taccctgtgt ttgtaggcgg ggaaggcgcg gtcgccctcc cagatcgtga tataggttgt
      961 ctcgccgacg gttttggacg tatgcgcaag tatcggaggg gccgtctcgc cgattccctt
     1021 gaagtgaccg tacacgttcc cgaggcgaag catgatgggg ctgagatggt attttttgga
     1081 ggagtgatcc tgaatgacga agttctgctc ctgcatcgtc cggagcagct tataggcgcc
     1141 gctcttgccg atgccggcat ggacggcgat ctcggaaaga ccgagctcgt agggcggttc
     1201 gccgagacgt ttcagaagaa gcaaggcttt ttccagactg ctcacgcgta cacctcgttt
     1261 ccttacagga aatgaagttt cccatagaag aatttactgc actatatcag ctactcagtg
     1321 atttgtcaaa aatcttttct t
//
LOCUS       DAOPKU010000721         1341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_119378_length_1341_cov_3.556765, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000721 DAOPKU010000000
VERSION     DAOPKU010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119378_length_1341_cov_3.556765"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PK849_15270"
     CDS             <1..886
                     /locus_tag="PK849_15270"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HPE67543.1"
                     /translation="VENVSGLLSAGSGAHYRKLHSALVDRGYDCGAIMLDASRFVPQS
                     RPRVFVIAVERGRRIPEELVGSAPCWLHSKAAAELGKTLPGWIWWRSEEPPKRHTRLE
                     DILEPDVPFDKDDVLRLIPERHREKLEEHATVYATGYRRTRQGRQQLELRFDGIAGCL
                     RTPEGGSSKQFIVLKKDGEVHARLLTVRETARLMGAPDSFRLPGSANDGYKAMGDAVA
                     LPVARFIGEQFLLKIAEAAYGDRQASNAELGRRVVYEHSLFDETNRSAAIAKIVDRYN
                     DIVAAAETDPSLRIEFER"
     gene            893..1309
                     /locus_tag="PK849_15275"
     CDS             893..1309
                     /locus_tag="PK849_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HPE67544.1"
                     /translation="MSDVFDKDARSKIMRKVRSKENRSTELRLMKLFKDNGITGWRRN
                     YPVVGHPDFVFPEYKVAVFVDGCFWHGHDCRNTRPQDNEEYWQKKRERNIRRDKEITA
                     RFESRRWTVLRIWECELRKKRIEETLMRIRQALERV"
BASE COUNT          313 a          372 c          432 g          224 t
ORIGIN      
        1 ggtcgaaaac gtctccggcc ttctttcagc aggaagcggc gcacactaca ggaaactgca
       61 ttcggcgctc gtcgacagag ggtatgactg cggcgcgatc atgctggacg cgtcccgttt
      121 cgtgccgcag tcgcgcccca gggtgttcgt catcgccgtc gagcgcggac gccgcattcc
      181 ggaggaactt gtcggttccg ctccgtgctg gctgcacagc aaggcggccg cggagctggg
      241 gaagacgctg cccggctgga tatggtggcg ttcggaagag cctccgaagc gccatacgcg
      301 actggaggac atcctcgaac cggacgttcc gttcgacaaa gacgacgtcc tccggctgat
      361 tccggagaga cacagggaaa agctcgaaga acacgccacg gtctacgcga ccggataccg
      421 gaggacccgc cagggacgtc agcagcttga actgcgcttc gacggcatcg ccggctgctt
      481 gaggacgccg gagggcggaa gcagcaagca gtttatagtg ctcaaaaagg acggcgaggt
      541 tcatgcacga ctgctcaccg tccgcgaaac agcccgtctg atgggcgcgc ccgacagttt
      601 caggcttccc ggcagcgcca acgacgggta caaggccatg ggcgacgccg tggcgcttcc
      661 cgtcgcacgc ttcatcggcg aacagttttt gctgaagata gcggaggcgg cgtacggtga
      721 tcgacaggcg tccaacgcag agttgggaag acgcgtcgtc tacgaacata gtcttttcga
      781 cgagacgaac cgcagcgcgg cgatcgcgaa gatagtggac aggtacaacg acatcgtcgc
      841 cgctgccgag accgacccaa gccttcgcat cgagttcgag aggtaacgcg ccgtgagcga
      901 cgtattcgac aaagacgctc gctcgaagat aatgcggaag gtgcgatcaa aagaaaaccg
      961 gtcgacggaa ttgcgtttga tgaaactatt caaagacaac ggaattacag ggtggcggcg
     1021 caactacccg gttgtaggac atcccgattt cgtctttccc gaatacaaag tggccgtctt
     1081 tgtggacggc tgcttctggc acgggcacga ctgccggaat acgcgccctc aagacaacga
     1141 ggagtactgg caaaagaagc gggagcgcaa catacgccgc gacaaggaga taaccgcccg
     1201 tttcgaaagc cgaaggtgga cggtgctgcg aatttgggag tgcgagttgc ggaaaaaacg
     1261 tatcgaagag acgctgatga gaatacggca ggcgcttgag cgggtgtgaa gtgaaacatg
     1321 cgatgcacat tcttcccagg g
//
LOCUS       DAOPKU010000722         1335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_120247_length_1335_cov_4.037500, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000722 DAOPKU010000000
VERSION     DAOPKU010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120247_length_1335_cov_4.037500"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..494)
                     /locus_tag="PK849_15280"
     CDS             complement(138..494)
                     /locus_tag="PK849_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPE67545.1"
                     /translation="MAYVKERKQFGKPISAFQNTQFQLAEMQTRIEAARHLVYDAAAT
                     KDSGARFSVEAAMAKLYASQTAMEVTTKCVQLHGGYGYMRDYPVERMMRDAKITEIYE
                     GTSEVQLMVVAGALLK"
     gene            complement(686..1246)
                     /locus_tag="PK849_15285"
                     /pseudo
     CDS             complement(686..1246)
                     /locus_tag="PK849_15285"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
BASE COUNT          282 a          411 c          301 g          341 t
ORIGIN      
        1 gaccggcctt gtcgtcagga ttgacgatac tgggaacgcc ttcgcgcatc aatgttccgg
       61 tcacgggatc cagccgcact tcattggtat ccggcacctg tttcacacat acaatgatct
      121 tcatgcgtct caccccttta tttcagcaag gcgccggcga caaccatcag ctgcacttcg
      181 ctggtacctt cgtagatctc cgtgatcttc gcgtcgcgca tcattctctc cacggggtag
      241 tcgcgcatat atccgtatcc gccgtgaagc tgcacgcatt tcgtggtaac ttccatagcc
      301 gtctgagagg cataaagctt ggccatcgcc gcctcgacgg aaaatctggc tccggaatcc
      361 tttgtcgctg cggcgtcata gaccagatgt ctcgcagcct cgatccgcgt ttgcatttcg
      421 gcaagctgga actgcgtgtt ttgaaaagcg gagatcggct tcccgaactg cttccgctct
      481 ttgacgtagg ccacggttca tccaacgccc cctgcgcgac tccgagcgcc tgagctccga
      541 ttccgatgcg tcctccatcc aaagtcccca tcgctatacc gaaccctttc ccttccttgc
      601 cgagcatatt ctccttagga accaggcaat tctcaaagat aagctcgcac gttgaagaac
      661 cccgtatccc catcttcttc tctttcttgc ctacggaaaa ccccgggaat tccttctcga
      721 cgatgaaagc cgaaatacct tttgttccga ggctcttgtc ggtcatcgca aagataatat
      781 acacatcggc ctgtccggcg ttggtaataa atatcttgct gccgttcaga acataatttt
      841 ctccgtcaag gacagccttc gtctgttgcc cggccgcatc ggtccccgct ccgggctccg
      901 taagcgcaaa agcgccgagc tttcttccgg agagaagatc gggaagatat ttttcttttt
      961 gctcaggggt accatacttg gcgatcggat cgcaccccaa cgacgtgtgc gcagaaacga
     1021 tgactcctgt cgtcgcgcaa aatcggctca tttcttcaac gcagagaata taggtcagag
     1081 tgtcgcaccc ctgcccttcc cattctttcc cgtacgggat gccgaggaag ccgaatcggg
     1141 ccatcttttc caccgtctcg gaaggaaacc gttcgttttc atccacttcc tgcgccaagg
     1201 gctttacttc cttttccgcg aaacgacgaa aaagttgctg cgccattcgc tgttctttac
     1261 tcagtgcaaa gctcatctat tcgccctcca atcatattcg caaacacttg agtttgttaa
     1321 aaaacacgat ggaga
//
LOCUS       DAOPKU010000723         1335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_120258_length_1335_cov_3.553906, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000723 DAOPKU010000000
VERSION     DAOPKU010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120258_length_1335_cov_3.553906"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK849_15290"
     CDS             <1..205
                     /locus_tag="PK849_15290"
                     /inference="COORDINATES: protein motif:HMM:NF025021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPE67546.1"
                     /translation="EVIRLLLEAGTFVNQVDSFGETALHAALEALARHNDAKDIVRMI
                     TEAGGETVFPVEGKRFDSNSERR"
     gene            212..907
                     /locus_tag="PK849_15295"
     CDS             212..907
                     /locus_tag="PK849_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67547.1"
                     /translation="MITFGAHPKGGLVFTGNGVHVSSPVLQDTMEKKERENELFIANY
                     RLLWENRGRIFATPELYDAKLSFPALSCAYFGGGPLSLGILLELWDIGYWTVPCPECG
                     GTCRVTYWCGSPLSGMNYWWGFCLDCGVRIHKNIYKRSDEVNNGRGFSRESHRALELI
                     KKHSKPIVVKDPPVPSDFRTWSKKCRERYESGAVIPVKEPEKEIIPVDDTKSLEEVIE
                     LLSRSAADSAEGS"
     gene            915..1307
                     /locus_tag="PK849_15300"
     CDS             915..1307
                     /locus_tag="PK849_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67548.1"
                     /translation="MQWEKTTGRKMHCCEGTINGVPARFTVHTGRIETGRSRMFIGYF
                     ASVEIDGEPVTGADSSGIVFALRNCAEKLRARGVVLDAPALSERFYESGLSENSGWGY
                     MRDGDDEPVHYIDRTKKYTRPPRYDSKS"
BASE COUNT          310 a          389 c          417 g          219 t
ORIGIN      
        1 ggaggtgatc cggctgctgc tggaggcggg gaccttcgtg aaccaggtcg actcgttcgg
       61 agagacggcg ctccatgcgg cgctcgaggc gctcgcgcgg cacaacgacg cgaaggatat
      121 cgtgcggatg ataacggaag cgggcggaga aacggtcttt ccggtcgaag gaaaacgctt
      181 cgattcgaac tctgagagga gatgattgcc catgatcaca ttcggcgcgc accccaaggg
      241 cggacttgtg ttcacaggaa acggcgttca cgtaagctct cccgtgcttc aggacacgat
      301 ggagaaaaaa gagcgcgaga acgagctgtt catagccaac taccggcttc tttgggagaa
      361 caggggacgc atcttcgcca cacccgaact gtacgacgcc aagctctcct tccccgcgct
      421 ctcctgcgcc tatttcggcg gcggccccct ctccctcggc atcctgctgg aactgtggga
      481 catcggctac tggaccgtgc cctgccccga atgcggcgga acctgccgcg tgacgtactg
      541 gtgcggcagt ccgctgagcg gcatgaacta ctggtggggc ttctgcctcg actgcggcgt
      601 cagaatacac aagaacatct acaaacgatc ggacgaggtg aataacggca ggggattcag
      661 ccgcgagtcg cacagggcgc tggagctgat aaaaaaacac agcaaaccca tcgtcgtgaa
      721 agacccgccc gtgccctccg acttcagaac atggtcgaaa aagtgccgtg aacggtacga
      781 gagcggcgcc gtcatccccg tcaaggaacc ggaaaaagaa atcatcccgg tcgacgacac
      841 gaaatcgctg gaagaagtga tcgagctgct ttcgcgcagc gccgcggact ccgcggaagg
      901 gagctgagag aagaatgcag tgggaaaaaa ccaccggacg aaagatgcat tgctgcgagg
      961 gtacgataaa cggcgttccg gcgcggttca ccgtgcacac ggggcgaatc gaaacgggtc
     1021 gttcccgcat gttcatcggc tatttcgcct ccgtggagat agacggcgaa ccggtgacgg
     1081 gcgccgactc ctcggggatt gtcttcgcgc tcaggaactg cgcagaaaaa ctgcgggcgc
     1141 ggggcgtcgt gctggacgcc ccggcgctct ccgaacggtt ttacgaatcc ggactctccg
     1201 agaattcggg gtgggggtac atgagggacg gcgatgacga gcccgtccac tacatcgacc
     1261 gcacgaaaaa gtacacacgc cctccccggt acgattccaa gtcgtaagca gaccttcccg
     1321 gaagaacaaa cgcga
//
LOCUS       DAOPKU010000724         1333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_120615_length_1333_cov_2.125978, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000724 DAOPKU010000000
VERSION     DAOPKU010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120615_length_1333_cov_2.125978"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..>1333)
                     /locus_tag="PK849_15305"
     CDS             complement(115..>1333)
                     /locus_tag="PK849_15305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03986 family CRISPR-associated RAMP protein"
                     /protein_id="HPE67549.1"
                     /translation="NRKANKEWAFWKRHLGNEEGVPVFLLFEGKSIKSMGLALMYRLP
                     YTHSILETVAHTSGDHLDGKRPDFGEMLFGRVEDTDALRGRVSVETFVAEGHPSPLPR
                     VDTVLGAPKPTFYPNYVKQKANAEGTVNQYSTYMHKNAEIRGWKRYIVQPDAAQKNAP
                     RAPTQNVETFFQPLPQGTTFIGSIHLHNVKPAELGALLWSLTWGGDTSLRHSLGMAKP
                     YGFGSVALSITSSSIAWCNPLKGGAPSLDECKKLFIETMSTWLGQPWEKSDAVAAVKA
                     MANPSTRWSQELRYPHLDQRTNEFADFKKQQASLIPVVAERAGGQARQQPLSAPATNR
                     APTPAAQKKPADLFLEECATTSLKKIPERLKHHKLEPDKVSPDEKKRIYEKLKSKLAN
                     QPLYASLLKQWKP"
BASE COUNT          214 a          332 c          431 g          356 t
ORIGIN      
        1 ctggacgggc gggagccgat tgactgcgat tattgaggta gaggaagacg atatcggtag
       61 gtatgacgaa aggaagcccc gtacggggct tcctttcgcg tgtctacggg gttgttacgg
      121 tttccactgt ttcagaaggc ttgcgtagag aggctgattc gccagtttgc ttttgagctt
      181 ttcgtagatg cgttttttct catccggaga gactttgtcc ggttcgagct tgtgatgttt
      241 gagtctttcg ggtattttct tcaaggacgt cgtagcgcat tcttcaagga agagatcggc
      301 aggttttttt tgtgctgcgg gagtcggcgc tctgttcgtc gccggtgcgg aaagaggctg
      361 ctgccgcgcc tgtcctcccg ctctttccgc cactacgggg ataagcgacg cctgctgctt
      421 tttaaaatcg gcgaactcgt tggttctctg gtcgagatgg gggtagcgaa gttcctgact
      481 ccatctggtg gaggggttcg ccattgcttt gacggcagca acggcgtcgc tcttttccca
      541 aggctgtccg agccaagtgc tcatcgtctc tatgaagagt tttttgcact cgtcgagcga
      601 cggcgctccg ccttttaagg ggttgcacca tgcgatgctg ctcgacgtta tggagagcgc
      661 gacggagccg aatccatagg gtttggccat tccgaggctg tgtctgagcg acgtgtcgcc
      721 tccccatgta agactccaga ggagagcgcc cagttccgcg ggttttacgt tgtggagatg
      781 gatgcttcct atgaaggtcg tgccctgcgg aaggggttgg aaaaacgttt ctacgttctg
      841 cgtgggagcc cgcggagcgt ttttctgcgc ggcgtccggc tggacgatgt agcgtttcca
      901 accacgtatc tcggcgtttt tatgcatgta ggtggagtac tggttgacgg ttccttcggc
      961 gttcgctttc tgtttcacgt agttgggata gaatgtgggc ttgggtgcgc cgaggacggt
     1021 gtcgacccgc ggcagcggcg aggggtgtcc ctcggcgacg aatgtttcca cggagactct
     1081 gccgcgcagc gcgtcggtgt cctcgacgcg gccgaagagc atttctccga agtcggggcg
     1141 tttgccgtcc aggtgatccc ccgaagtgtg ggcgaccgtc tcaagtatcg agtgcgtgta
     1201 cggcaggcgg tacatgagcg cgagtcccat ggacttgatg ctctttcctt cgaagagcag
     1261 gaataccggg acgccttctt cgtttccgag gtggcgtttc cagaaggccc actccttgtt
     1321 ggcctttctg ttc
//
LOCUS       DAOPKU010000725         1332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_120667_length_1332_cov_4.265466, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000725 DAOPKU010000000
VERSION     DAOPKU010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120667_length_1332_cov_4.265466"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /gene="rhuM"
                     /locus_tag="PK849_15310"
     CDS             complement(<1..244)
                     /gene="rhuM"
                     /locus_tag="PK849_15310"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HPE67550.1"
                     /translation="MNSIRGTRFRMWATERIKEYIVKGFAMDDDRLKRGGGGAYFEEL
                     LARIRDIRSSEKVFLVFFPKGGDAPCSARSSAISRVH"
     gene            complement(511..642)
                     /locus_tag="PK849_15315"
     CDS             complement(511..642)
                     /locus_tag="PK849_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67551.1"
                     /translation="MLGATIGDITDSRFGLDNRWSEEFELFTEVCFATDDRSSKTGG"
     gene            1026..1298
                     /locus_tag="PK849_15320"
     CDS             1026..1298
                     /locus_tag="PK849_15320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HPE67552.1"
                     /translation="MVNATVNIRLEAEDKKEAEKIFATLGLTTSEAIRLFYRQTILRR
                     GLPFSVDIPNDVTVAAMEAARRGELEAGGFEELGSRISSNERRGRV"
BASE COUNT          292 a          361 c          355 g          324 t
ORIGIN      
        1 cgtgaacccg cgatatcgcc gatgatcgcg ccgagcatgg ggcgtcgcct ccttttggaa
       61 aaaaaaccaa aaacaccttc tccgaagaac gaatgtcgcg gatgcgggcg agaagctcct
      121 cgaaataggc gcctccgcct cctcttttga gacgatcatc gtccatcgcg aaccccttga
      181 cgatgtactc cttgattcgt tcagtcgccc acattcgaaa ccgagtgccc cgaatgctgt
      241 tcactctgta tccgaccgaa atgatcgcat ccagattgaa gtgttccact tgataggttt
      301 tgccgtccgc tcaagctcgc cgctctcgaa gacattcttc aagtggcggc tgataccgga
      361 tttatcgata gcgaagagat cggccatctg tgaaaggctc aaccatagtg tctcgttctg
      421 gaggcgagcc tcgattcgaa tgcttccgtt ttccgcttga tagagcagaa actcggtttc
      481 caaaggcgct tgaccggacc ctctcatacg ctaaccccct gttttcgaag atctgtcgtc
      541 cgtcgcgaag cagacctccg tgaagagttc gaactcttcg ctccagcggt tgtcgagccc
      601 gaaccgggag tccgtgatat cgccgatggt cgcgccgagc atggagcgtc gcctcttccg
      661 accaggaggc ttttcccccg acgacgaagt gttcccgcaa acgccggcga tacctttttt
      721 cccgtttcca gcatcggtaa tcccgacctg ttcttcatca tatgtggatt tatcttagcg
      781 taacggagag cgtcgttgtc ctcgattcaa tatatacgtt caagtcttat tgtcaagttt
      841 tttgtccagt cgctgttttc aggcgcgctc ttcccagtta cggcgtgtac ggcggtactc
      901 cgcctttctt tgccgccttc tcattcgatc atcagaaaaa ggagagtgtc tggacgccgg
      961 tatcgggaat ccctctagag atgtaacgca aaaagcaata caaatgcgtt cggggggtga
     1021 gcggtatggt gaatgcgacc gtaaatatca gattggaagc cgaggataag aaagaggccg
     1081 agaagatctt tgcaacactc ggcttgacga cgagtgaagc gattcgtctt ttctacaggc
     1141 agacgattct gcgccggggg cttccttttt cagtcgatat ccccaacgac gtcaccgtcg
     1201 cggctatgga ggcggcacgt cgcggcgagc tggaagcggg cggtttcgaa gagctgggaa
     1261 gcaggatttc ttcaaatgaa aggcggggaa gagtatgatc atcggcacgc tggctgcttg
     1321 cgaacggtat ta
//
LOCUS       DAOPKU010000726         1330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_120982_length_1330_cov_2.874510, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000726 DAOPKU010000000
VERSION     DAOPKU010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120982_length_1330_cov_2.874510"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1308
                     /locus_tag="PK849_15325"
     CDS             142..1308
                     /locus_tag="PK849_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPE67553.1"
                     /translation="MSDRKKFFWIAAFFLTFYFIPEGNERVLAGLAESVAMMADYARE
                     HVLLCLIPAFFIAGAISVFVSQQSVMKYLGAKAKKWIAYSVASVSGTVLAVCSCTVLP
                     LFAGIYTRGAGLGPATAFLYSGPAINVLAIVLTARVLGLELGIARAVGAVLFSVIIGL
                     LMAAIFGKEEKARQEGFAQIPEDEGGRPLWKTVVYMASMVVFLVFANWAAPKTSNAFW
                     EGVYNLKWILAGAGIAVTLLTSWLWFTRDERQDWYTSTWGYALQVMPLLFGGVLVAGF
                     LLGRPGHEALIPSRYVAMLVGGNSLAANFFASISGAFMYFATLTEVPILQGLLGAGMG
                     KGPALALLLAGPALSLPSMLVINSILGPKKTATYVVLVVALSTAAGMLFGIIAS"
BASE COUNT          208 a          394 c          440 g          288 t
ORIGIN      
        1 tacgagaagg tgaaggatct gcttttgagg gcgaaggggt aagctgacac actgcacccc
       61 gcctcggcgg ggatttcccc ttgctcttta agtgaataaa acgccacata atggcgatac
      121 tgtatgaaat ggagagagat tatgagcgac cgtaaaaagt ttttttggat agccgcgttt
      181 tttctgacgt tctacttcat ccccgagggg aacgagcgcg ttcttgcagg actcgcggag
      241 tccgtggcca tgatggctga ctacgcgcgg gagcacgtgc tgctctgcct gattcccgcg
      301 ttcttcatcg ccggggccat ctcagttttc gtgagtcaac agtcggtgat gaagtacctg
      361 ggggcgaagg cgaagaagtg gatcgcgtac tcggtggcat cggtctccgg cacggtgctg
      421 gcggtctgct cctgcacggt attgccgctt ttcgcgggga tctacacgcg gggcgccggg
      481 ctcggccccg ctacggcctt cctctactcc ggccccgcga tcaacgtgct cgcgatcgtc
      541 ctcacggcgc gggtgctcgg gcttgaactc ggcatcgcgc gcgcagtggg cgcggtgctc
      601 ttcagcgtga tcatcggcct gttgatggcc gccatcttcg gcaaggagga gaaggcccgg
      661 caggaggggt tcgcgcagat tcccgaggat gagggcggcc gtcctttgtg gaagaccgtc
      721 gtctacatgg cgagcatggt cgtcttcctc gtcttcgcca actgggctgc gcccaagacg
      781 tccaacgcct tctgggaggg cgtgtacaac ctgaagtgga ttctcgccgg agcggggatc
      841 gccgttacgc tccttacctc gtggctgtgg ttcacccgcg acgagcggca ggactggtac
      901 acgtcgacat gggggtacgc gctccaggtc atgccgctgc tcttcggcgg cgtgctcgtc
      961 gcggggttcc tcctgggacg gccggggcac gaggcgctca tcccgagccg gtacgtcgcc
     1021 atgctggtgg gcggcaactc gttagcggcg aacttcttcg cgtccatctc cggcgcgttc
     1081 atgtacttcg ccacgctgac cgaggttccc attctgcagg ggctgctcgg cgcggggatg
     1141 ggaaaaggcc ccgcgctcgc cctgctgctt gccggaccgg cgctctcgct gccgagcatg
     1201 ctggtcatca acagcatcct cgggccgaag aagaccgcga cgtatgtagt tctggtggtg
     1261 gccctctcga cggcggccgg aatgctcttc gggattatcg cttcctaatg ggagtttcgg
     1321 gcatttgccc
//
LOCUS       DAOPKU010000727         1325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_121670_length_1325_cov_2.865354, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000727 DAOPKU010000000
VERSION     DAOPKU010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121670_length_1325_cov_2.865354"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PK849_15330"
     CDS             complement(<1..672)
                     /locus_tag="PK849_15330"
                     /inference="COORDINATES: protein motif:HMM:NF039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HPE67554.1"
                     /translation="MTLKDIFKKPVDRPIEGVIKADDEASLRLEIEEYVLTNEIEKRL
                     ESFLAAYNSCDGSNGAWISGFFGSGKSHLLKILALLLENREIDGTAALELFLPKCGDN
                     EILRGDLKRASSIPSKSILFNIDQKADIISKTQIDALLAVFVKVFDEMCGYYGKLGHI
                     AQFERDLDSRGLYGKFTSEYLACAGIPWQKGREQALLEGRNIARAYAAAGGGEEAEAS
                     GILDKY"
     gene            complement(687..>1325)
                     /locus_tag="PK849_15335"
     CDS             complement(687..>1325)
                     /locus_tag="PK849_15335"
                     /inference="COORDINATES: protein motif:HMM:NF020329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HPE67555.1"
                     /translation="RIRPQGDDAMKKDIAARPVQERLQHLFSVISGRRFLEKQGLGNE
                     VPFFICPYPPEESVEMDRMRRSLVRQLENAGVRILEIDLYDLSVEILKERGIWDRILE
                     METSTSKEELNELLQGVLDPKANLVPAIAARMAGAEFDVLFISGVGEVFPCIRSHSVL
                     NNLQSTAKEQPTVMFFPGAYTHSSESGASLDLFGRLHNDKYYRAFNIFHCEA"
BASE COUNT          248 a          389 c          375 g          313 t
ORIGIN      
        1 gtacttgtcc agtatcccgg atgcttcggc ctcttcgcct ccgccggcgg cggcgtatgc
       61 gcgggcgatg ttgcgcccct ccagcagcgc ctgctcgcgc cccttttgcc agggtatccc
      121 ggcgcatgcc aggtattcgg atgtgaattt cccgtagagt ccgcggctgt ccagatcacg
      181 ttcgaattgg gcgatatgcc cgagcttgcc gtagtagccg cacatctcgt cgaagacctt
      241 gacgaagacc gcgagcaggg cgtcgatctg cgttttgctg atgatatccg ccttctggtc
      301 gatgttgaac aggatgcttt tcgaggggat ggacgaggct ctcttgaggt cgccgcgaag
      361 gatctcgttg tcgccgcact tggggaggaa gagttcaagc gccgccgttc cgtcgatctc
      421 gcggttctcc agaagcaggg cgaggatctt cagcagatgc gatttacccg atccgaagaa
      481 tcccgatatc caggccccgt ttgagccgtc acagctattg tacgccgcga gaaaggattc
      541 caggcgtttt tcgatttcgt tggtgaggac gtactcctct atctcgaggc gaaggctcgc
      601 ttcgtcgtcg gccttgataa ccccttcgat cggacggtcg accggttttt tgaaaatgtc
      661 cttcagagtc atttcgatcc cctctctcat gcttcgcagt ggaagatatt gaatgccctg
      721 tagtatttat cgttatgcag ccttccgaag aggtccagcg aggcgcccga ctcggacgag
      781 tgtgtgtagg ctcccgggaa gaacatgacg gtgggttgct ccttcgcggt actctgaaga
      841 ttgttgagca cactgtgcga ccgtatgcag ggaaagactt cgccgacccc ggaaatgaag
      901 agcacgtcga actcagcgcc cgccatccgg gcggcgatcg cgggcacaag gttcgccttc
      961 ggatccagca ctccctgtaa gagctcattc agctcttcct tggaagtcga ggtctccatc
     1021 tcgaggattc gatcccagat accccgctct ttgagtattt cgaccgagag gtcgtacagg
     1081 tcgatctcca gaatccggac tcccgcgttt tccaactgcc tgaccaagct gcgccgcata
     1141 cgatccatct ccacggactc ctccggcggg taggggcaga tgaagaacgg cacttcgttg
     1201 cccagcccct gtttttcgag gaacctcctg ccggagatca cggaaaaaag atgctgcaat
     1261 ctctcctgca caggacgtgc cgcgatatcc tttttcattg cgtcatcccc ttgaggtcgg
     1321 attcg
//
LOCUS       DAOPKU010000728         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_122003_length_1322_cov_6.179953, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000728 DAOPKU010000000
VERSION     DAOPKU010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122003_length_1322_cov_6.179953"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1322)
                     /locus_tag="PK849_15340"
     CDS             complement(<1..>1322)
                     /locus_tag="PK849_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPE67556.1"
                     /translation="FALIFLKGFPEDEEGMKVFTGAPELEAFFEMHRESDAFRKGIES
                     SMYDEIFQPAERLQFAGEERRILFAPAHDGETGDVTGTAVFLFEPAGFDKFLVLMRGL
                     FILALVIFTAVSAVASYSRDPVLGYVILGLFAIVGVFVAYPLFEAVRLSFIQDGKFTL
                     DTWKSILTARQYLTALQGSITLGVFTATASTIVGFLFAFVISRTGIGGKKFFSAMATL
                     PIISPPFSLTLSIILLFGNNGLITKRILALEGFSIYGLGGLTLVQTMGMFPIAFLTMI
                     GVLEAIDSTLEDAAMNLRATKWETFRTVTFPLAVPGLLSAWLLVFTNSLADFANPLIL
                     AGSFRVLSVESYMEVTGMNRLGHGAALSLLLLLPTLSAFLVQRYWVSRKSYVTVTGKP
                     SGRMTDLVSPGLKKVLVALMCLIVLFLLSLYGTIIAGCFVKNWGIDYS"
BASE COUNT          283 a          355 c          458 g          226 t
ORIGIN      
        1 ggagtagtcg atgccccagt tcttcacgaa gcaccctgcg atgatggtgc cgtagaggct
       61 cagaaggaag aggacgatga ggcacatcag cgcgacgagg acttttttca gccccgggga
      121 gacaagatcg gtcatcctcc ccgacggctt gcccgtcacg gtgacgtacg acttcctgct
      181 cacccagtag cgctggacga ggaacgccga gagcgtcggc agcagaagga gcagcgagag
      241 cgccgcgccg tgaccgagcc ggttcatccc ggtgacctcc atgtacgact caaccgagag
      301 gacgcggaag ctccccgcga ggatcagcgg gttggcgaag tcggcaagcg aattcgtgaa
      361 gacgagcagc cacgcgctca acagccccgg gaccgcgagc gggaaggtga cggttctgaa
      421 ggtttcccac ttggtggcgc ggaggttcat cgccgcgtct tccagggtgg agtcgatggc
      481 ttccagaacg ccgatcatgg tgagaaaggc gatggggaac atccccatgg tctgtaccag
      541 cgtcagtccg ccgagcccgt agatgctgaa tccttcgagc gcgagtatcc tctttgtgat
      601 aaggccgttg ttgccgaaga gaaggatgat cgagagcgtc agggagaagg ggggcgagat
      661 gatcgggagc gtcgccattg cggagaagaa cttctttccg ccgatcccgg tcctggagat
      721 gacgaaggcg aagaggaagc cgacgatggt ggaagccgtt gcggtgaaga cgccgagcgt
      781 gatgcttcct tgcaaggcgg tgaggtactg ccgtgcggtc aggatggact tccacgtgtc
      841 gagcgtgaac ttcccgtcct ggatgaagga gagtcggacg gcctcgaaga gcgggtaggc
      901 gacgaagacg ccgacgatgg cgaagaggcc gaggatgacg tacccgagaa cggggtcgcg
      961 gctgtaactt gccacggcgg agacagcggt gaagatgacg agggccagaa tgaagagccc
     1021 tctcatgagg acgagaaact tgtcgaagcc tgcgggttcg aaaagaaaga ccgctgtgcc
     1081 ggtcacatcg cccgtctcgc cgtcgtgggc cggggcgaag agaatgcgcc gctcttcgcc
     1141 ggcgaactgg aggcgctcgg cgggctggaa gatctcgtcg tacatgctgc tttcgatccc
     1201 cttgcggaag gcgtcgcttt cccggtgcat ttcaaagaat gcttcgagtt cgggagcgcc
     1261 ggtgaagacc ttcattccct cctcgtcctc ggggaatccc ttgaggaaga tcagggcgaa
     1321 at
//
LOCUS       DAOPKU010000729         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_122094_length_1322_cov_1.878453, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000729 DAOPKU010000000
VERSION     DAOPKU010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122094_length_1322_cov_1.878453"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..576)
                     /locus_tag="PK849_15345"
     CDS             complement(247..576)
                     /locus_tag="PK849_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67557.1"
                     /translation="MAKTKIKIIMEKSAASGAGGCGAYIPGGESIEGRQDAVSAIREL
                     LSKTKETFGESVDISVMDPRSIFSFVDNFRYNIKGTAPAWIVNGKKVFEGVPEWDEWE
                     KILREAF"
     gene            737..>1322
                     /locus_tag="PK849_15350"
     CDS             737..>1322
                     /locus_tag="PK849_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67558.1"
                     /translation="MDEKGMIICSADIDEGLTLQIWTKGAVPRLVVLNRAKNTRKLMP
                     FSWLEREDRTISLKGAKGKTNSYTVASLEEPVRRMLYQYAQDPAFKGLLWHSVIFMSD
                     LMHTPRAVFDRAEFAMLHEDKRCRLWLLDLTDGEANGYFRPFFPRTAPEEIFGDEPGV
                     QAHGGKNVADLKKTGITGKLASVLPSRWYDTPRIS"
BASE COUNT          309 a          345 c          353 g          315 t
ORIGIN      
        1 gctgaagcac gcggtgaaac agaaaaccgc ccgagaaggg cgaagcgcat ggatgttcga
       61 gcgccggcgc acagataccg agacgatggt cgtggagaat gcgtcatgac tctgaaggga
      121 aaatttctcg cccttgcggc gggaatcata ctcaccgcaa ccatccttgc aggagagatc
      181 ctgtaaccga ctggacgccg gagagcgcaa aggctctccg gctcgtcctg ctcttcggag
      241 tgcggcttaa aacgcctctc gcaggatttt ttcccactcg tcccattcgg ggacgccttc
      301 gaaaactttt tttccgttca cgatccacgc aggggcggtc cctttgatgt tgtagcgaaa
      361 gttgtcaacg aaggaaaaaa tactccgggg atccattacg gaaatgtcta cactctcgcc
      421 gaatgtctcc ttggttttcg agagcagttc gcggattgca gaaactgcgt cttggcgtcc
      481 ctctatggac tcccctccgg gaatataggc tccgcacccc cccgcgccgc ttgccgcact
      541 tttttccatg atgatcttta tctttgtctt cgccatggta ccttcacctc ctcgtgtact
      601 gtcaatatca tcataataca cgaaaaattc atatcgaaaa aacgagattt ttactgaaag
      661 ctcttttttc ccctttgcat ttaagaaaaa agacgtcata atagacggta ttagtattga
      721 tgaaggagtg cggagtatgg atgaaaaggg catgataata tgctcggctg atattgacga
      781 ggggttgacc ctccagatat ggaccaaagg cgccgtaccc cgcctcgtgg ttctgaacag
      841 ggctaagaat acccggaaac tcatgccttt cagctggctc gaaagagagg atcggacgat
      901 ctcccttaaa ggggccaaag gcaaaaccaa ctcatataca gtagcgagtc ttgaggaacc
      961 tgttcgcagg atgctgtacc agtatgctca ggatccggcg ttcaaagggc ttctctggca
     1021 ttcggtgatc tttatgtccg atttgatgca tacgccgcgt gcggtcttcg acagggcgga
     1081 gttcgcaatg ttgcatgagg acaagcgatg ccgcctgtgg cttcttgatc ttacggacgg
     1141 ggaggcgaac gggtatttcc gccccttttt cccccgaacg gccccggagg agatcttcgg
     1201 agacgagccg ggcgtccagg ctcatggcgg taaaaatgtt gcggatctga agaagacagg
     1261 gatcaccgga aagctcgctt ctgttcttcc ctcgcgctgg tacgatacgc cgcgtatttc
     1321 gg
//
LOCUS       DAOPKU010000730         1321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_122127_length_1321_cov_4.852291, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000730 DAOPKU010000000
VERSION     DAOPKU010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122127_length_1321_cov_4.852291"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PK849_15355"
     CDS             <1..597
                     /locus_tag="PK849_15355"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPE67559.1"
                     /translation="KDLRIGFCKGCFACWTATPGRCVQRDDMDMVLEKMDAADTLLWA
                     TPLYHFGMTAMLKAVMERTLPLADPHIVKAGDVYTHPGRSGKRFPQYLLFSNCGFPDQ
                     GHFDGLRAHFESLAKASGKRGTIPAVFRAAGELLRQEEVRPHIQWYFDALRQAGKEYV
                     EHGGISGESMEVLHRDLVPQEMYISQANAHWDSVLKGK"
     gene            688..1275
                     /locus_tag="PK849_15360"
     CDS             688..1275
                     /locus_tag="PK849_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67560.1"
                     /translation="MNPLGMIISFAPWLVLKILTSIPFSDPLTRLKTALVIAILICIY
                     QSRSAVKGFIFWGTIAFFSFSFVTVVLMTNMWVIDRLGLLSQLTMSAIAWGSILFKRP
                     FTLDYSKQYVPSEFWTHPAFLRKNYLITGVWGCYFLLGVAINEIRIYEPQISHLLLEV
                     LDNASMLAIILYTSHASKRRSVSASSENGQDTPVE"
BASE COUNT          263 a          377 c          369 g          312 t
ORIGIN      
        1 aaggatttga gaataggctt ttgcaagggg tgtttcgcct gctggacggc aactcccggc
       61 agatgcgtcc agcgcgacga catggacatg gtgctcgaga agatggatgc cgcagatacg
      121 ctcctctggg cgacgccgct ctaccacttc ggcatgaccg ccatgctgaa ggccgtcatg
      181 gaacggacgc tccccttggc cgatccgcat atcgtcaagg ccggcgatgt gtacacccac
      241 cccggccgct ccgggaagcg ctttccgcag tacctcctct tttccaactg cggcttcccc
      301 gaccagggac atttcgacgg tctcagggcg cattttgaat cgctcgcaaa agcgagcggg
      361 aagcgcggga caattccggc ggtctttcgc gcggcaggag agctcttacg gcaggaggag
      421 gtccggccgc acatacagtg gtacttcgac gcgctgcgcc aagcggggaa agagtacgtc
      481 gaacacggcg gcatctcagg cgaatcaatg gaggtattgc accgggatct cgttccgcag
      541 gagatgtaca tttcgcaggc gaacgcgcac tgggactcgg tgttgaaggg gaaataagaa
      601 ggcgactgtg ctattattac cctgccgtaa gcgagcgtac cgcctttcgt ccgtcggccc
      661 tacactattt tcttgggagg ttgtactgtg aatcctttgg gtatgattat ttccttcgct
      721 ccgtggcttg tgctcaagat cttgaccagc attccttttt ccgatccgct gaccaggctg
      781 aaaacggcgc ttgtcatcgc gattttgata tgcatctacc agtcgcgaag cgcggtgaag
      841 ggctttattt tctggggaac catcgcgttc ttctccttca gcttcgtcac cgtagtgctg
      901 atgacgaaca tgtgggtcat cgatcgcctc ggtctgctgt cgcagctgac catgtccgcc
      961 atcgcctggg gatcgatcct ctttaaacgg ccctttacgc tcgactactc caaacagtac
     1021 gtcccgtccg agttctggac gcaccccgcc tttctccgga agaactacct gatcacgggc
     1081 gtatgggggt gctacttcct tctgggcgtc gcgatcaacg agatacggat ctacgagccg
     1141 cagataagcc acctgcttct cgaagtgctc gacaatgcct cgatgctcgc catcatcctc
     1201 tatacgtcgc acgcttcgaa gcggcgcagc gtttcagctt cgtcggaaaa cggacaggat
     1261 actccggtag aataatgatt gtgtgttatg ctgtttgagg aaccattttt attaaagtct
     1321 c
//
LOCUS       DAOPKU010000731         1320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_122286_length_1320_cov_3.735178, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000731 DAOPKU010000000
VERSION     DAOPKU010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122286_length_1320_cov_3.735178"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PK849_15365"
     CDS             complement(<1..876)
                     /locus_tag="PK849_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67561.1"
                     /translation="MHPSAHKGVLNGFFKGCVLFFLAAALGFVIPSRACAYAGDIRVE
                     GVPFWLRPSVERSLRSVWNEIAQRSGFSGEDRRALLSVVAERLFVGYSSSVLLSESGV
                     IVTLSPVSPVAWTVEIKTPSLSSPVEEWFAENVAELGGTLERMLALLPLEALSWADIA
                     LNEAIEDVCVPLLPGWNPSLIVRLEGRGIASGPPPADEKRILQISFSPKPPLVLAIVP
                     SISSSTLPVAFRSDLREKILRTLAPVVGLPIEWAAKNKLRIEALAAEALFDTNTVSNA
                     RATVDVSFSPEQLSPV"
     gene            complement(899..>1320)
                     /locus_tag="PK849_15370"
     CDS             complement(899..>1320)
                     /locus_tag="PK849_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPE67562.1"
                     /translation="TPIKGLEDLLNCQIGGDPDNPAVFAARMRIGFSSGTLPTASLYS
                     LGGSSTLRGYDGGEYEGTKMFLANLELRVPIEKAFGLVLFYDTGNAWLEESMNFSDLH
                     DAWGVGVRVKTPLGNFRLDHATGENETRTHFGFGEMF"
BASE COUNT          328 a          389 c          364 g          239 t
ORIGIN      
        1 cacgggggag agttgctcgg gggagaaaga aacgtcaacg gtcgcccggg cgttcgagac
       61 cgtattggta tcgaacagcg cctctgcggc aagagcttct atacggagct tgttttttgc
      121 cgcccactct attggcagac cgacaaccgg agcaagcgtc ctcaatatct tctccctcag
      181 gtcggagcga aaggctacgg gcagcgtgga ggaagagatg gacggaacga tagccagcac
      241 caagggaggt ttgggagaaa aagagatctg cagaatacgt ttttcgtccg ctggaggcgg
      301 tccgcttgca attcccctcc cttcaaggcg cacgatgagc gacgggttcc agccgggaag
      361 cagaggaacg catacgtctt caatcgcttc attcaatgct atatcggccc aagaaagcgc
      421 ctcgagcggg agcaacgcaa gcatccgttc cagcgtcccg ccgagctccg cgacattttc
      481 agcaaaccac tcctcaaccg gggaggaaag cgacggcgtt tttatctcca ccgtccacgc
      541 aacgggcgaa accggagaaa gggtgacaat cacaccggac tcggagagca aaaccgagga
      601 ggagtagcca acgaaaagcc tctccgcaac gaccgagagg agcgcacggc gatcttctcc
      661 ggaaaatccg gagcgctgcg caatctcgtt ccatacagaa cgcagactcc tctcgacgct
      721 tggacgaagc cagaacggga cgccttcgac ccggatatcc ccggcgtatg cgcacgctcg
      781 ggaggggata acgaagccaa gggctgcggc aaggaaaaac agcacacagc ccttgaagaa
      841 tccgttcaat actcccttgt gcgcagaagg atgcacatcg gcgcctcctt cgggcgctct
      901 agaacatttc tccaaaaccg aaatgcgttc gtgtttcatt ctctcccgtg gcatggtcga
      961 gccggaagtt ccccagagga gtctttaccc tgacgccgac tccccacgca tcatgaagat
     1021 cggaaaaatt catgctctct tcaagccatg cgttccccgt atcgtagaag agaacaaggc
     1081 caaacgcctt ctcgatagga acacgcagtt caaggttggc caggaacatc ttcgttccct
     1141 cgtactcccc gccgtcatac cctctgagcg tgctcgaacc gccgagcgaa tagagactgg
     1201 cggtcggcaa ggttccggaa gaaaaaccga tacgcatcct cgcggcgaaa acagcaggat
     1261 tatccgggtc tccgccgatt tggcagttca agagatcttc gagccccttt atcggcgtat
//
LOCUS       DAOPKU010000732         1317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_122711_length_1317_cov_3.744057, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000732 DAOPKU010000000
VERSION     DAOPKU010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122711_length_1317_cov_3.744057"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PK849_15375"
     CDS             complement(<1..642)
                     /locus_tag="PK849_15375"
                     /inference="COORDINATES: protein motif:HMM:NF019824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HPE67563.1"
                     /translation="MFGLEERALVSPREMEERSRLLASRGLTVPGNESVILGFFEEER
                     LVATGSLVGNILQGIAVDRELEGEGVAATVTSALLKKAMQMGRRQLFLYSKPDEAKRF
                     EALGFTLLATASVERTGLGASLLEWGSEGIDKWRAAIATKAEGGPEGAGVAVVNCNPF
                     TLGHYRLLEYAAARTPRLYVLVVEEDRSLFPFDVRFRLVKEGVSGLENVVALRG"
     gene            complement(664..>1317)
                     /locus_tag="PK849_15380"
     CDS             complement(664..>1317)
                     /locus_tag="PK849_15380"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868776.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPE67564.1"
                     /translation="IVDDSMVLLPPEDGSSVEIRRGPNIKPLPEFLPMADEIQGEVLL
                     KMGDNITTDHILPAGSRIMSLRSNLPAISEYCFNIVDSSFPERARKAGGGILVAGENY
                     GQGSSREHAALSPLFLGVKAIITVSYARIHRQNLVNAGILPLLFEDRADLEKIAQGDR
                     LELVNLHSCLEGEFVEVRNVTTGVRIRTRHGLSRRQREILLAGGALNAARVALTKEP"
BASE COUNT          239 a          469 c          371 g          238 t
ORIGIN      
        1 gccgcgaagc gctacgacgt tctccagtcc ggacactccc tccttgacaa ggcggaaacg
       61 cacatcgaag gggaagagcg accggtcctc ctccacgacg aggacgtaga gccggggcgt
      121 tcgtgcggcg gcgtactcca acaggcggta gtgccccagc gtaaacgggt tgcagttcac
      181 caccgcgacg ccggccccct ccgggccgcc ctccgccttc gtggctatcg ccgctctcca
      241 tttgtctatt ccttcggagc cccactccag cagcgaagct ccaagacccg tccgctccac
      301 cgatgcagtc gccagcagcg tgaagccgag cgcctcgaag cgcttcgcct cgtccggttt
      361 cgagtacagg aagagctgcc tgcgccccat ctgcatcgct tttttcagca gagcggaggt
      421 caccgtcgcg gcgacgccct ccccctcaag ttcccgatcg accgcgatcc cctgcagaat
      481 attccccacc agggagccgg tcgcgacaag acgctcttcc tcgaaaaatc ccaggatcac
      541 agattcgttt cccggaaccg tgaggcccct cgacgccagc aatcgcgagc gttcctccat
      601 ctccctcggc gagaccagcg cccgctcctc cagtccgaac atgcgcgctc cccctttcga
      661 ccgttacggt tctttcgtca gcgcaacacg ggcggcgttg agcgccccgc cggcgagcag
      721 aatctcccgc tgtctccgcg aaagcccgtg gcgcgttctg atacgaactc ccgtcgtcac
      781 attccgcacc tcgacaaact ccccttcgag gcaagagtgc agatttacca gctccagccg
      841 gtccccttgc gcgatcttct caagatcggc cctgtcctcg aagagcagcg gaagaatccc
      901 cgcgttcacc aggttctgcc tgtggatcct tgcgtacgag acggtgatga tcgccttgac
      961 gccgagaaag agcggcgaga gggccgcatg ctccctgctc gacccctgcc cgtagttctc
     1021 ccccgcgacg aggattcctc ctcctgcctt gcgcgcgcgc tccgggaagg aggagtcgac
     1081 gatgttgaag cagtactcgg agatggcggg gaggttcgag cgcagcgaca taatccggct
     1141 gcccgcggga aggatgtgat cggtcgtgat gttatccccc atcttcaaca gcacctcgcc
     1201 ctgtatctca tcggccatgg ggaggaactc gggaagcggc ttgatgttcg gccccctgcg
     1261 gatctcgacg gaactgccgt cctccggagg aaggagcacc atcgaatcgt cgacgat
//
LOCUS       DAOPKU010000733         1317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_122726_length_1317_cov_3.100634, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000733 DAOPKU010000000
VERSION     DAOPKU010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122726_length_1317_cov_3.100634"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PK849_15385"
     CDS             complement(<1..1140)
                     /locus_tag="PK849_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPE67565.1"
                     /translation="MTQIHAIINGKPITGEPGMTILQAARANGIYIPTLCDHPALSPF
                     GACRVCLVEAEKNPKLLTSCTTPITEGMVVHTNTPRVIEARKAVVELILVRHPLDCFS
                     CSSNGKCELQEVAYELGIKGSRFADPGDTNDSHRLEDANPFYVRDMDKCILCGRCVRA
                     CDEQALYHAIDFQGRGINTSIQPPVGSPLEESDCVFCGQCVQVCPVGALFEKSSVGRG
                     RPWELKKVTSACSYCGVGCELEINVNAKTGRIAGVTTDYESPTALNKGRSCVKGRFAW
                     QFVHSEDRLTAPLVREDGAFRTATWDEALSLVANKLSEIKSAHGPDSLAFFSSARCTN
                     EENYLMQRLAREVFGTNNVDHCARLCHSSTVAGLAETLGSGAMTND"
BASE COUNT          228 a          396 c          442 g          251 t
ORIGIN      
        1 gtcgttggtc atcgcgccgc tgccgagcgt ttcggccagt cccgcaacgg tcgagctgtg
       61 gcagagacgg gcgcagtggt cgacgttgtt cgtgccgaag acctcgcgcg cgaggcgctg
      121 catcaggtag ttctcctcgt tggtgcagcg ggcggaggag aagaaggcga gagagtcggg
      181 gccgtgcgcg ctcttgatct ccgaaagttt gttcgcgacg agcgagagcg cctcgtccca
      241 ggtcgccgtg cggaaggcgc cgtcctcgcg gacgagcggc gcggtcaagc ggtcttcgct
      301 gtggacgaac tgccacgcga agcgcccctt cacgcacgat ctccccttgt tgagggccgt
      361 cgggctttcg tagtccgtcg tgacgcccgc gatcctgccc gtcttcgcat tgacgttgat
      421 ctccagttcg caccccacgc cgcagtagga acaggccgac gtcaccttct tcagctccca
      481 cggacggcca cggccgacgg aggatttctc gaaaagcgcg cccaccggac agacttggac
      541 gcactgtccg caaaagacgc agtccgactc ctccagcgga gagcctaccg gcggctggat
      601 ggaggtgttg atgccgcgcc cctggaagtc gatcgcgtgg tagagggcct gttcatcgca
      661 cgcgcggacg cagcggccgc agaggatgca cttgtccata tcgcggacgt agaaggggtt
      721 ggcgtcctcc agtcggtgcg agtcgttcgt gtcgccgggg tcggcgaagc gcgagccctt
      781 gattccgagt tcgtacgcga cctcctgaag ctcgcacttc ccgttgctgg agcaggagaa
      841 acagtccagc ggatgtcgga cgaggatcag ctcgacgacc gccttgcgcg cctcgataac
      901 cctcggcgtg ttcgtgtgca cgaccatgcc ctccgtgatg ggagtggtac aggaggtcag
      961 gagcttgggg ttcttctccg cctcgacgag acagacgcgg catgcgccga acggactgag
     1021 cgcggggtgg tcgcagagcg tcggaatgta tatgccgttg gctcgggccg cctgaaggat
     1081 cgtcatgccc ggctcgccgg ttatcggttt tccgttgatg atcgcgtgaa tctgtgtcat
     1141 tttttcagat catctccttg atggatatgc ttcttcatgc cccttcgggg gcatgttcgt
     1201 tcttcgcggg gacgcgggat cttcggaacg agccgaaggc gagcgccgcg aagaggagaa
     1261 cgccgaagag agggaaagac ttccccggaa tgaaacataa aaaaccggcc cccgcag
//
LOCUS       DAOPKU010000734         1315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_122971_length_1315_cov_3.811905, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000734 DAOPKU010000000
VERSION     DAOPKU010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122971_length_1315_cov_3.811905"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..>1315)
                     /locus_tag="PK849_15390"
     CDS             complement(10..>1315)
                     /locus_tag="PK849_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE67566.1"
                     /translation="VTFIGMVGFLLYINWKLTLITFAILPLTVLILDRAAKKLRGVGH
                     AIQSELATLSSVVQEAFGAIRIVRSFATEDLELARFRNSNRDNFRAVMHGVQVQSALA
                     GIIEVVLIAALALILWLGGKDVVGGELTPGELIAFLGYLGFLVQPIRVFTSVVSSMQI
                     GLAATDRIFALLDAPEDIVSPDNPTPLGAIEGSVTFRHVCFSYVKGQEVLRGINLDVR
                     PGEKIAIVGPTGSGKSTLADLIPRFYDPDSGTVYVDGTDVRTVNLRELRKQIGVVHQD
                     SLLLRGSIAFNISYGLPGLSLEEIVKAAEIAGIHSFIESLPKGYDTEVGERGVTLSGG
                     QRQRIAIARAVVRNPRILILDEATSSLDMEVERLVQKAMNKAMEGRTSFVIAHRLSTV
                     RNADRIVVLDEGRIAEQGTHEELLARNGLYHRLCVLGETAAP"
BASE COUNT          253 a          406 c          419 g          237 t
ORIGIN      
        1 ccgcgccgtt catggagcag cagtctcccc gaggacgcac aggcggtgat agagaccgtt
       61 gcgcgcgagc agctcttcat gcgtcccctg ctcggcgatg cgcccttcgt caagcacgac
      121 gatcctgtcc gcgttccgga ccgtggagag gcgatgcgcg atgacaaagg aggtgcgtcc
      181 ctccatggcc ttgttcatcg ccttctggac cagacgctcg acctccatgt cgagagatga
      241 ggtcgcctcg tcgaggatga ggatcctcgg attccgtacc acggcccggg ctatggcgat
      301 gcgctgccgc tgtccgccgc tgagagtaac cccgcgttcg ccgacttcgg tgtcgtaccc
      361 tttagggagc gattctatga aggagtgaat accggctatc tccgcggctt taacgatttc
      421 ctccagggag agtccgggca ggccgtacga tatgttgaag gcgattgagc cgcgcagcag
      481 cagcgaatcc tggtgcacca cgccgatctg ctttcgaagt tcccgaagat tgacggtgcg
      541 cacatccgtg ccgtcgacgt agaccgtccc gctgtcggga tcgtagaagc ggggaatgag
      601 atcggccagc gtggatttgc ccgagcctgt cgggccgacg atggcgatct tctccccggg
      661 acggacgtcg aggttgatcc ccctgagaac ctcctgcccc ttgacgtacg aaaaacagac
      721 gtgcctgaag gtgacgcttc cctcgatcgc cccgagcggc gtcgggttgt ccggggagac
      781 gatgtcctcc ggcgcgtcga gcagggcgaa gatcctgtcc gtggcggcaa gccctatctg
      841 catggaactg accaccgagg tgaacacacg aatgggctgc acaaggaacc ccagataacc
      901 gagaaaggcg atcagttcgc cgggagtgag ctcgcccccg acgacgtcct tgcctccgag
      961 ccagaggatg agcgcaagcg ccgcgatgag gacgacctct atgattccgg cgagggccga
     1021 ctgcacctgg acgccgtgca tcactgcgcg gaagttgtcg cggttgctgt tcctgaaccg
     1081 ggcgagctcc agatcctcgg tcgcgaagga gcgcacaatc ctgatcgcgc cgaatgcctc
     1141 ctgcacgacc gaggagagcg tcgccagctc gctctgtatg gcgtgcccca ccccgcggag
     1201 cttcttcgcg gcacggtcca ggatgagcac ggtaagcggg agaatagcga aggtgataag
     1261 cgtgagcttc cagttgatgt acaggagaaa ccccaccatt ccgatgaagg tgacc
//
LOCUS       DAOPKU010000735         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_123664_length_1310_cov_3.831076, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000735 DAOPKU010000000
VERSION     DAOPKU010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123664_length_1310_cov_3.831076"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..>1310
                     /locus_tag="PK849_15395"
     CDS             203..>1310
                     /locus_tag="PK849_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPE67567.1"
                     /translation="MQPGVPGNTALLADGGDVLVRCLSAFQQRRAPLRYAFSFGEAVD
                     YSPLEAASSILQSDSQTDFYVFILKELREGRKFLDFASDAAKKGKRTAVLLLRRPQTS
                     SREETAALKQFGVVLLNDIQEIVDGALAFLALKNSGLSGGKECAYHTSSDELSMLIKK
                     EAERAEFTLQESARATSPLLIDVLSGSDSLNTVQRRKQRIEQSGRLPVFSSLADNALF
                     EEQLYSAKIPLIPGIRRCFNALQLVFGSGPAVCPAPSEGAHPTKCPLHPRPTEYDAKV
                     LLRAYDIPVAREALCHSLPEALTAAETIGYPIALKVMSPSILRKTEARVIALDVQNEE
                     ELKNAYGRTLEKARIANPSARIRGVLVQEMVHGGT"
BASE COUNT          291 a          342 c          338 g          339 t
ORIGIN      
        1 tcccattccg gatacactgt ttttcgcagg aaaaggtcct gaagaccgga agatgctcga
       61 aagatgtttt gaacttccca ttcccttcct tgtgcttggc ggccgttggc ctcgaaagct
      121 ctctctctct ctttgctcca gagcaagtga aaatggaatg cgtattcttg gaccccgaag
      181 tgaaggaatt atcgtaccct gtatgcagcc gggcgtccct ggaaacaccg cattgcttgc
      241 tgatgggggt gacgttctcg ttcgctgcct ctccgctttt caacaaagaa gagcgcctct
      301 caggtacgca ttctccttcg gcgaggcagt ggactattct ccgcttgaag ctgcctcctc
      361 gattcttcaa tccgattctc aaaccgattt ctatgtgttt attctgaaag aattacgtga
      421 agggcgtaag tttcttgatt ttgcatcgga tgccgcaaag aaaggaaaaa gaactgctgt
      481 tcttcttctt cgccggcccc aaacgagttc ccgtgaggaa actgctgccc tgaagcaatt
      541 cggcgtggtt ctcctgaacg atattcagga gattgtcgat ggggccctag cctttctggc
      601 tttgaagaac agtggccttt ccggggggaa ggaatgtgca taccatacct cctcggacga
      661 actttccatg ttgataaaaa aagaagcgga aagagctgaa tttactcttc aagagagcgc
      721 tcgggcgacg tcgccccttc tgatcgatgt cctttctgga tccgattctc tgaataccgt
      781 tcagcgaaga aagcaacgta tcgaacagtc aggccggctt cctgtgtttt catctctggc
      841 ggacaacgca ttgttcgaag aacagttgta ttcggcgaaa atcccgctta tccccggtat
      901 tcgccgttgc tttaacgctt tgcagctggt cttcgggagc ggcccggcgg tgtgcccggc
      961 tccgtccgaa ggcgctcatc ctacaaagtg tccactgcat ccccgtccga ctgaatatga
     1021 cgcaaaagtg cttcttcgag cgtacgatat acctgttgcg agagaagctt tgtgccattc
     1081 gctacccgag gctttgaccg ctgcggagac aataggctac cctattgctc tcaaggtgat
     1141 gtctccatct attttgcgta aaacggaggc tcgcgttatc gccctggatg ttcagaacga
     1201 ggaagagctc aagaacgcct acggacgaac gcttgaaaaa gcgcgcattg ccaatccgtc
     1261 tgccaggatt cggggagttc ttgttcagga aatggtgcac gggggaacgg
//
LOCUS       DAOPKU010000736         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_123673_length_1310_cov_3.537052, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000736 DAOPKU010000000
VERSION     DAOPKU010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123673_length_1310_cov_3.537052"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PK849_15400"
     CDS             <1..211
                     /locus_tag="PK849_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HPE67568.1"
                     /translation="LPQSVSGERDCILSLRVAAPWRNRCIVVKDGEREVARKKEMRLH
                     PAEMIRIPLKKEALSGCSSLEVTVE"
     gene            208..600
                     /locus_tag="PK849_15405"
     CDS             208..600
                     /locus_tag="PK849_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HPE67569.1"
                     /translation="MSTRELTCVVCPGGCTIVVDVDNDETPVVTRIQGNNCPRGEAWA
                     RQEVENPMRTIASSVPVRGGDFPLVSVRTLRPIPLTKIREVMEEIRTLSLEAPVEIGS
                     VVLASPAGCDTEVVATRRVKAAAPCAGA"
     gene            664..>1310
                     /locus_tag="PK849_15410"
     CDS             664..>1310
                     /locus_tag="PK849_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE67570.1"
                     /translation="MKKVLIAVLVLFALCAAIGGMTSEAEAKSYRINIATATTGGAYY
                     PIGNAMAQIWSRNLEDVRRASAQSTAGTPQNVELMKDGEVQIAFGQNGICYYAYYGKG
                     IYDGKPPYTDLRGMFTLYPNVMHWLVRKGSGIQSIADFKERKFVPGQVASATEINSRE
                     MMEAYGLNYMKDAGEVNVTADFVGYNEAADLLKNAQTDGTHIAGGLPTAAIMDALS"
BASE COUNT          292 a          353 c          423 g          242 t
ORIGIN      
        1 gctgccgcag agcgtctcgg gggaacgcga ctgtatactt tccctcaggg tggcggctcc
       61 gtggcgtaat cggtgtatag tagtgaagga cggcgagcgg gaggttgccc ggaagaagga
      121 gatgcgcctc caccccgccg agatgatccg tattccgctg aagaaagaag cgctttcggg
      181 ctgttcttcg ctggaggtga ccgtggaatg agtacccgcg agctgacctg cgtcgtctgt
      241 ccgggcggct gcacgatcgt tgtcgatgtc gataacgacg aaacgcctgt cgttacgagg
      301 atacagggga acaactgccc tcgcggagag gcgtgggcgc gccaggaagt ggaaaacccc
      361 atgagaacca ttgcgtcaag cgttccggtg cgtggaggag attttcctct cgtcagcgtc
      421 agaacgcttc ggccgatacc gttgacgaag atacgggagg tgatggagga aatccgtacg
      481 ctgtctctgg aggctccggt ggagataggg agcgtggtgc tcgcgtcccc cgccggctgt
      541 gataccgagg tcgttgcgac ccggagagtg aaggctgcgg ccccctgcgc cggggcgtaa
      601 ggcagggtgc gcatgcccga actgaatggc gaagaagtta tacatcagaa ggagggaatc
      661 gttgtgaaaa aagttcttat tgcagttctt gtcctgtttg ctctgtgtgc ggcaatcggt
      721 ggtatgacca gcgaggccga ggcgaaatcg taccgaatca acatcgcgac cgccactacc
      781 ggcggagcct actaccccat cgggaacgcg atggcgcaga tctggtccag aaaccttgaa
      841 gacgtccgca gggcgtccgc acagtctaca gcgggcactc cgcagaatgt cgaacttatg
      901 aaggacggcg aagtgcagat cgccttcgga cagaacggta tctgctacta tgcatactac
      961 ggcaagggaa tctacgacgg taagcccccg tacaccgacc tccggggtat gttcacgctc
     1021 taccccaacg taatgcactg gctcgtccgg aaggggtccg ggattcagtc gatcgccgat
     1081 tttaaggaga gaaagttcgt tcccgggcag gttgcaagcg caacggagat caacagccgg
     1141 gagatgatgg aagcctacgg cctgaactat atgaaggacg cgggagaagt gaacgtaacc
     1201 gccgacttcg tcggatacaa cgaagccgcc gaccttctga agaacgccca gaccgacgga
     1261 acgcatattg ccggcggcct tcccacagcc gcgatcatgg atgcgctctc
//
LOCUS       DAOPKU010000737         1302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_124878_length_1302_cov_3.235766, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000737 DAOPKU010000000
VERSION     DAOPKU010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124878_length_1302_cov_3.235766"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PK849_15415"
     CDS             <1..894
                     /locus_tag="PK849_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67571.1"
                     /translation="DWRKRGISAVLSFAGGREIAFLRPHLGEAGPVFLSAFGETANIY
                     NGEGVPDTMVFALDLFRDFRIAAFTAYAAELFPSGSVLAVLGDKLDPALERYSRNLGD
                     MFFDRGFLVAHFWIPGGGMDSFKMIESEAIASGAKALVSWAGSMVVRDVWRSTRRKSN
                     AFEIWYGGVPQPLLLSFNGVVTADQDYPVRTDPALKSLGRDIWRRHNVTVKDPAIAGR
                     AYAACQWLFDAFKRAGSPVPAALAGMLPLAEGLVFGSQALSINPATHRPAERAVAFMI
                     VEGREFHPVKTLLVRGPDYLP"
     gene            945..>1302
                     /locus_tag="PK849_15420"
     CDS             945..>1302
                     /locus_tag="PK849_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPE67572.1"
                     /translation="MKLRDIASQIRAEVLSGEELLDDIDIECAYGADLMSDVLAFARP
                     GSLLLTGLTNIQIVRTAQMLDLPAVVFVRGKKPQEAAVKLASQIKMPILLCHMSMFES
                     CGILFQKGILPCHIPDR"
BASE COUNT          244 a          385 c          396 g          277 t
ORIGIN      
        1 gactggcgga agcgcggaat ctccgcagtg ctttcctttg ccggggggag agaaatcgcc
       61 tttctccggc cgcatctcgg agaagcggga cccgtctttc tctctgcttt cggagaaacc
      121 gcgaacattt acaacggcga aggcgtcccc gatacaatgg tcttcgcgct ggatctgttc
      181 cgcgacttca gaatcgccgc gttcaccgcg tatgcggcgg agctcttccc ttccgggagc
      241 gttcttgcgg tcctcggcga caagcttgac cccgcgcttg agcggtattc ccggaacctc
      301 ggcgatatgt ttttcgatag gggcttcctt gtggcgcact tctggattcc cggcggagga
      361 atggattcct tcaagatgat agaatcggaa gccattgctt caggcgccaa agccctcgtc
      421 tcctgggcgg ggtcgatggt ggtcagggac gtctggcgaa gcacccgtcg caagagcaac
      481 gctttcgaga tctggtacgg cggcgttccg caaccgcttc ttctctcctt caacggcgtt
      541 gtgacggccg accaggacta ccccgtgcgg actgatccgg ctctgaagtc gctgggcagg
      601 gacatctgga gacggcataa cgtcaccgta aaagaccccg caatcgcggg gcgcgcgtat
      661 gcggcctgcc aatggctttt cgacgccttc aagcgggcgg gctctcccgt cccggccgcc
      721 ctggcgggta tgctgccctt ggccgagggg cttgtcttcg gttctcaggc gctttcaatc
      781 aatcctgcaa cacaccgtcc cgccgagagg gccgtggcgt tcatgatcgt ggaggggaga
      841 gagtttcacc ccgtcaaaac gctccttgtc agaggaccgg attacctgcc gtaaaggccc
      901 ctgtcccggg gaagtgttca agataatcta gaggaggaag tagaatgaaa ctgcgtgata
      961 ttgcgtctca gatacgcgcg gaggtgctca gcggcgaaga gctgctcgac gatatcgaca
     1021 tagagtgcgc atacggggcc gacctgatga gcgacgtgct tgcgttcgcc cgccccggat
     1081 cgctgctcct gaccggattg accaacatcc agatagtacg gaccgcgcag atgctcgacc
     1141 ttccggccgt tgtcttcgtc cgggggaaaa agccgcagga ggcggcggtc aagctggcct
     1201 cccagatcaa aatgccgatt cttctctgtc acatgagcat gttcgagtcg tgcggcatac
     1261 tgtttcagaa aggcattctc ccttgtcata taccggacag ag
//
LOCUS       DAOPKU010000738         1301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_125024_length_1301_cov_3.239968, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000738 DAOPKU010000000
VERSION     DAOPKU010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125024_length_1301_cov_3.239968"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PK849_15425"
     CDS             complement(<1..1239)
                     /locus_tag="PK849_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HPE67573.1"
                     /translation="MMHELLSGRDGRSRGERIVTLLCVSMSLFHMYTAGIGLLETAVQ
                     RAVHLGFAMTLVFLLHPFSKPKGVRAPDLLFAALSVVCTGYIALFFNDIAMRGGKALP
                     HDILLGALAMLLVLEAGRRVLGRTLPALALLFLLYCVFGRYAPSIFAHRGYSAARIIQ
                     HMYLTPEGIFGVALGVSSTFVFMFILFGSFLSAGGGARFFNDLALALSGRSTGGPAKV
                     AILASGLLGTINGSSVANVATTGTFTIPLMKKTGYTPEDAGAIEACASTGGQLLPPIM
                     GAGAFIMSEFLSVPYARIVAAATIPALLYYGAILANVHLRARRENIGRLDPNALPRAL
                     DILKRDGHLLLPIAAVVFLLLRGYTPLMAAFRGILCLMAVCALRRHTRFTMRDIVAAL
                     EDGAKNALGTAIACAVVGFIV"
BASE COUNT          271 a          402 c          444 g          184 t
ORIGIN      
        1 tacgatgaag ccgaccactg cgcaggctat cgccgtgccg agcgcgtttt tcgcgccgtc
       61 ctcgagggcg gcgacgatgt cgcgcatcgt gaatcgcgtg tgccgccgca gcgcgcagac
      121 ggccatgagg cagagaatcc cccggaacgc ggccatgagc ggggtgtagc cgcgtagtag
      181 aagaaagacc accgcggcga tcgggagaag aagatgtccg tctcttttca gaatgtcgag
      241 agcgcggggc agggcgttcg ggtcgagacg cccgatgttc tctctccgag cgcgcagatg
      301 gacgttcgcc agaatcgcgc cgtagtacag cagcgcggga atggtcgccg cggcgacgat
      361 acgggcgtac ggcacgctca aaaactcgct catgatgaac gcccccgccc ccatgatcgg
      421 gggaaggagc tgcccgccgg tcgacgcgca cgcctcgatc gcccccgcgt cttccggtgt
      481 atagccggtc ttcttcatca gcggaatggt gaaggtccct gtggtggcca cgttcgccac
      541 cgaggagccg ttgatcgttc ccagcaggcc ggaggccaga atcgccacct ttgcgggacc
      601 gcccgtgctc cgaccggaga gggccagcgc caggtcgttg aagaaacgcg cgcctcctcc
      661 cgccgacaga aacgagccga agaggatgaa catgaagacg aaggtgctgg agacgccgag
      721 cgccacgccg aagatcccct ccggagtcag gtacatgtgc tggatgatcc gcgccgccga
      781 gtacccccgg tgcgcgaaga tcgacggagc gtagcgcccg aagacgcagt agagcagaaa
      841 gaggagcgcc agagcgggga gcgttctgcc gagaacgcgc cgccccgctt cgaggacgag
      901 cagcatcgcg agcgcgccga gcagaatgtc gtgcgggagc gccttccctc cgcgcatggc
      961 gatgtcgttg aagaagaggg cgatatagcc ggtgcagacg acggagagag ccgcgaacag
     1021 caggtccgga gcgcggactc ccttcggctt cgagaatggg tggagcagaa agaccagcgt
     1081 catcgcaaag cccaagtgca ccgccctctg aacggcggtc tcgagcagac cgatgcccgc
     1141 ggtgtacatg tgaaacagcg acatcgacac gcagagcagc gtgacgatgc gttcgccgcg
     1201 cgatcgtccg tcgcgcccgg agagaagttc gtgcatcatt gcggcactcc tttgaaaaat
     1261 cggtacgcgg agaggacgga aaacgccctc tccgcgaagg g
//
LOCUS       DAOPKU010000739         1300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_125140_length_1300_cov_3.616867, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000739 DAOPKU010000000
VERSION     DAOPKU010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125140_length_1300_cov_3.616867"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..>1300)
                     /locus_tag="PK849_15430"
     CDS             complement(214..>1300)
                     /locus_tag="PK849_15430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HPE67574.1"
                     /translation="EALASLPEESKGFPRYIVVGTYKGTRERPAIHFSGHYDVVPAGG
                     GWTVDPYGGLVADGKIFGRGASDMKSGIAAQIFALETLQHAGIPLKGSFVSSAVPDEE
                     TGGQPGMGYLVRSGRLTKENTDYCIITEPLDPDMICLGHRGTLWFTLAFKGVQSHGSM
                     PSEGLNPIEGMRALLERIDRRIRPQLERVSKYPVSPERSRKSTLCITTIQAGSKVNTI
                     PDTCSASFDWRLIPEQSVEWAKGEILSICDDLVREGVILEYAYTPIIEADPTMVDENQ
                     PLVDALKTCGKAVLGKEMGINFSPGMDDQRFAVHEGGLGAAVVYGPGRLALAHKSDEH
                     IAVDDLVKGVEIMAAVAAELLGTEERQ"
BASE COUNT          212 a          425 c          392 g          271 t
ORIGIN      
        1 cgtcacgtag gcgaattttc tgccctcttc cttgtacttc ttgaaatcaa gcaccgacat
       61 cttcttcttc ataaattgcc ctcctctttc ctgccgagat agcggcagcg tatgcgtgcc
      121 gctctgtatc gtttcattcc gccgcgcggc ggacgtcaga agacgcgccg gcggcgaatg
      181 aacgccaaaa actttgccgt gatcacgatc cggctactgt cgctcctccg tcccgagcag
      241 ctccgccgcg accgccgcca tgatttcaac gcccttgacg agatcgtcga cggcgatatg
      301 ctcgtctgat ttatgagcga gcgcaagacg cccgggcccg tacaccacag ccgccccgag
      361 gccgccttcg tggacggcga agcgctgatc gtccattccg gggctgaagt tgatccccat
      421 ttccttcccc aggacggcct tcccgcaggt cttcaatgcg tcgaccagcg gctggttttc
      481 atcgaccatg gtggggtcgg cttcgatgat cggagtatag gcgtactcga ggatgacgcc
      541 ctcgcgcaca aggtcgtcgc agatggagag aatctccccc ttcgcccact ccacgctctg
      601 ctccgggatg aggcgccagt cgaagctcgc ggaacaggtg tcgggaatcg tattgacctt
      661 cgatccggcc tggatggtcg tgatgcagag ggtgctcttc cgggagcgtt cggggctgac
      721 ggggtatttg ctgacgcgct ccagctgcgg acggatgcgc cggtcgatgc gttcgagcag
      781 cgccctcata ccctcgatgg ggttcagccc ctccgacggc atgctcccgt ggctctgcac
      841 tcccttgaag gcaagggtga accagagggt tccgcggtgc cccaggcaga tcatgtcggg
      901 gtcgagcggc tcggtgatga tgcagtagtc ggtgttctcc ttcgtcagcc ttccgctccg
      961 gacgagatac cccatgccgg gttgtcctcc ggtctcctcg tcggggaccg cgctggagac
     1021 gaagctcccc ttcaagggaa tgcccgcgtg ctggagcgtt tcgagggcga agatctgcgc
     1081 ggcgatgccg gacttcatgt cgctcgcgcc gcgcccgaaa attttcccgt cggcgaccag
     1141 gcctccgtac ggatcgacgg tccagccccc tccggccggg acgacgtcgt agtgcccgga
     1201 aaagtggatc gccggacgct ctctcgtccc tttatacgta ccgacgacga tgtatctggg
     1261 gaaccctttc gactcctcgg ggagcgacgc gagcgcctcc
//
LOCUS       DAOPKU010000740         1295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_125968_length_1295_cov_2.334677, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000740 DAOPKU010000000
VERSION     DAOPKU010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125968_length_1295_cov_2.334677"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1295
                     /locus_tag="PK849_15435"
     CDS             <1..>1295
                     /locus_tag="PK849_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67575.1"
                     /translation="SGTTTYVAEQWGRRWITVDTSRVALALARARIMGARYPYYLLAD
                     SEEGKLKEAEITRNAPSEKPVYGDIRQGFVYERVPHITLKSIANNAEIDVIWEKWQEV
                     LEPLRVQLNEALETRWEEWEIPRSAAPEWPESVRAIHSEWWRARIGRQQEIDASIAAK
                     ADFEYLYDKPYEDRKTVRVAGPFTVESVSPYRTLAVDENDEMIDPANRKNPEENTGKD
                     FAHVVLENLKMAGVQQAHKEDRIVFSSLAPWPGEYICGEGRYVESGDSGVERRAAVFI
                     GPEFGTVSRPDLVQAAREAADAGFDVLIACAFSFEAHTTEFTKLGSVPVLKARMNAEL
                     HMGDSLKSTGKGNLFVIFGEPDIEISHEPGGKLRVHLKGVDVFHPQTGEVRSDGPEGI
                     ACWFVDTDYDEESFFVRHAYFLGANDPYGALRTTLRAEI"
BASE COUNT          284 a          389 c          419 g          203 t
ORIGIN      
        1 gttccggaac aaccacctac gtcgccgaac agtggggcag gcgctggatc accgtcgaca
       61 cttcgcgagt cgctctggcg cttgcacgcg cccgcatcat gggagcgcgc tacccctact
      121 acctcctcgc cgacagcgaa gaggggaagc tgaaagaggc cgaaataacc cggaacgccc
      181 cctccgaaaa gccagtctac ggagacatcc gccaaggctt cgtctacgag cgggtacccc
      241 acatcacgct gaagtccatc gccaacaacg ccgagatcga cgtcatctgg gagaaatggc
      301 aggaggttct ggagcccctc cgcgtccaac tgaacgaagc tcttgaaacg cgatgggaag
      361 agtgggagat cccccgttca gcagcgccgg aatggccgga aagcgtccgc gccatccatt
      421 cggagtggtg gcgggctcgg attggacgtc agcaggagat cgacgcctcc atcgctgcga
      481 aggcggactt cgagtacctc tacgacaaac cctacgaaga cagaaaaacg gtcagggtcg
      541 cggggccgtt cacggtcgag agcgtctctc cctaccggac gctcgccgtg gacgaaaacg
      601 acgagatgat cgacccggcg aacaggaaga accccgagga aaacaccgga aaggactttg
      661 cgcacgtcgt cctggagaac ctcaaaatgg ccggggtgca gcaagcgcac aaggaggaca
      721 gaattgtctt ctcctccctc gcgccgtggc cgggcgagta catctgcggc gaggggcgct
      781 acgtcgaatc aggagactca ggggtcgaac ggcgtgccgc cgtcttcatc ggtccggagt
      841 tcggcaccgt ctcacggcca gacttggtgc aggcggcgcg cgaagctgcg gatgctgggt
      901 tcgacgtgct gatcgcctgc gcgttcagct tcgaggcgca cacgacggag ttcaccaagc
      961 tggggagcgt acccgtgctc aaggcccgca tgaacgccga actgcacatg ggcgattccc
     1021 tcaagagcac cggcaagggg aatctgttcg tgatcttcgg tgagccggac atcgaaattt
     1081 cgcacgaacc cggcggaaag ctgcgcgtgc atctcaaggg agtggacgtc tttcacccgc
     1141 agaccgggga agtcagaagc gacggcccgg agggtatcgc ctgctggttt gtggatacgg
     1201 actacgacga ggagagtttc ttcgtcaggc acgcgtattt tctcggggcg aacgacccgt
     1261 acggggcgct gaggacgacg ctgagggcgg agatc
//
LOCUS       DAOPKU010000741         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_126217_length_1293_cov_3.420032, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000741 DAOPKU010000000
VERSION     DAOPKU010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126217_length_1293_cov_3.420032"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PK849_15440"
     CDS             complement(<1..497)
                     /locus_tag="PK849_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPE67576.1"
                     /translation="MAKKQYVHAVPNFSNGKDPEVFEAVVDQIRKAPGVKLIDYFPDA
                     DFNRTVIECLGEPKPLKAALLAMAGKSYDLIDMEKQQGKHPRIGAQDTIPLFPLRNIS
                     LEECAALAEEIGAEVWERYKVPVYFSGENARTPERKELSFIRKGQYEGLKEVAHLPER
                     APDLGP"
     gene            complement(588..>1293)
                     /gene="dctP"
                     /locus_tag="PK849_15445"
     CDS             complement(588..>1293)
                     /gene="dctP"
                     /locus_tag="PK849_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE67577.1"
                     /translation="DPIVNRDFFAQSKALNVMLNDIYREKGLSVLSYFTEGAMFWTAN
                     KAIRTPDDFKGVKIRVMPSELLVETYRAYGANPTPLSFTELYSGLQLNVVEGQENAPA
                     TIQEMKFMDVQKFLIGSRHNMYVMQHLVNVGFLDSLPEDVRKIVLDSVDEVRPYIHDV
                     QEELNKTRLQMMIDDFKSGQEYYELTHEEREAFKEIAKKADAKYFELSRNPEFAKELL
                     ETFRAEMAELESKRGK"
BASE COUNT          236 a          371 c          363 g          323 t
ORIGIN      
        1 ggccccagat cgggcgctct ctcgggaagg tgcgccactt ccttcagccc ttcgtactgc
       61 cccttgcgaa tgaaggaaag ttccttccgc tcaggcgtcc gcgcattctc tccgctgaag
      121 tagacgggga ccttgtaccg ctcccagacc tccgctccga tctcttcggc gagcgcggcg
      181 cactcttcaa gggagatgtt ccgcagcggg aagagaggga tggtgtcctg tgcgccgatg
      241 cgagggtgtt tcccctgctg tttctccatg tcgataagat cgtaggactt ccccgccatg
      301 gcaagaagtg ccgccttcag cggctttggc tcgccgaggc actcgatgac ggtacggttg
      361 aaatccgcgt cggggaagta gtcgatgagc ttgactcccg gcgcttttcg tatctgatcg
      421 accaccgctt caaagacttc aggatctttt ccgttgctga aattcgggac tgcatgcaca
      481 tactgtttct ttgccataat gcgttcctcc tgtcaatgac ccgttgcaca tcgatagaat
      541 gcttcatgaa aaggggggcc gaagcccccc tctgccgctt tcagccgcta ctttcctctt
      601 ttgctctcca gttcggccat ttcggcccgg aacgtttcaa ggagctcctt ggcgaactcg
      661 ggatttctcg aaagctcgaa gtacttcgcg tcggctttct tcgcaatctc cttgaacgct
      721 tctctctcct cgtgcgtcag ttcgtagtac tcctgtcccg atttgaagtc gtcgatcatc
      781 atctgcaggc gggtcttgtt cagctcttcc tgaacgtcgt gaatatacgg gcgtacctcg
      841 tcaaccgagt cgaggacgat cttgcgcaca tcctcgggaa gggaatcgag gaatccgacg
      901 ttcacaagat gctgcatgac gtacatgtta tggcgcgaac cgatgaggaa cttctggaca
      961 tccatgaact tcatctcctg gatggtcgcg ggggcgttct cctgcccctc cactacgttg
     1021 agctgaagtc cgctgtacag ttcggtgaag gagagcggcg tcgggttggc tccgtacgct
     1081 ctgtaggttt ccacgagcag ttcggagggc ataacgcgga tcttgacgcc cttgaaatcg
     1141 tcgggggtac ggatagcctt gttcgctgtc cagaacatgg cgccctcggt gaagtaggag
     1201 agaacggaga gccccttctc ccggtagatg tcgttgagca taacgttgag cgccttgctc
     1261 tgagcgaaga agtctctgtt cacgatgggg tcg
//
LOCUS       DAOPKU010000742         1290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_126624_length_1290_cov_4.006478, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000742 DAOPKU010000000
VERSION     DAOPKU010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126624_length_1290_cov_4.006478"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PK849_15450"
     CDS             <1..437
                     /locus_tag="PK849_15450"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HPE67578.1"
                     /translation="FDLYALPSDFPDYDQCERFEPYTRVEALENAFKLAIFDRRFLPY
                     IQIHEFEALILADPTKLDWEYLEHERAIRNLVDMVNEQNPELIDDGPETAPSKRILRE
                     IPEYDKATAGVSVAAHIGLPTLRKKCRHFDEWVTQLESLGKE"
     gene            563..>1290
                     /locus_tag="PK849_15455"
     CDS             563..>1290
                     /locus_tag="PK849_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPE67579.1"
                     /translation="MATLRSSRVLLDHIADESPAAPVRVKEAKERGVPVVGTFCVFTP
                     WELVRAVGGISVSLCSTSEKPIAAAEAHLPRNLCPLIKASYGFALTDTCPYFHFCDIV
                     IGETTCDGKKKMYEYLNRLRPVHVMQLPQTTEGETARALWRAETERLKSVLESFYGRT
                     ITDDALREAIRLKNRERRVLNRFFAVARLDPSPLTGLEILAVSDYLKYSMASEEALCV
                     VEQLTAALEEERRRGKGSQTAGRKR"
BASE COUNT          272 a          398 c          375 g          245 t
ORIGIN      
        1 tgttcgacct gtacgccttg ccctccgatt ttccggacta cgaccagtgc gaacggttcg
       61 aaccctacac acgggtggag gcgcttgaaa acgcgttcaa attggcgata ttcgaccgtc
      121 gttttctccc ttatatccag atacacgagt tcgaagcact gattttggcg gatccgacga
      181 aactcgattg ggagtatctg gagcacgaac gagccattcg caacctggtc gacatggtga
      241 acgagcagaa tccggagctt atcgacgatg gccccgagac ggcgccttca aaacgaattc
      301 ttcgggagat ccccgaatac gacaaagcga ctgccggcgt ttccgtagcg gcgcatatcg
      361 ggttgccgac cctgcgaaag aagtgcagac acttcgacga atgggtcact caactggaga
      421 gcctggggaa agaatgaggg agagtcatcg ttcccgttgt taaaaaaacc tctttcggtt
      481 ataattttta ttataataac ggcaaatgcg gcgccctatc atctcctctc gccgcctgct
      541 tcctgaaagg ggtttccgtt tcatggcaac gcttcgttca tcccgtgtcc tgctcgacca
      601 tatcgccgac gaatcgcccg ccgcgccggt gcgcgtgaag gaggcgaagg agcggggcgt
      661 tcccgtggtg ggaactttct gcgtcttcac cccctgggag cttgtccgcg ccgttggggg
      721 aatctccgtc tcgctgtgca gcaccagcga aaaaccgatc gccgcagccg aggcgcacct
      781 gccgcgcaac ctctgtccgc tgatcaaggc gagctacggc ttcgccctca ccgacacctg
      841 cccgtacttc cacttctgcg acatcgtcat cggcgagacc acctgcgacg gcaagaagaa
      901 gatgtacgag tacctgaacc gcctccgccc cgtccacgtg atgcagctcc cccagacgac
      961 ggagggggag acggcccgcg cgctctggcg ggcggagacg gagcgcctga aaagcgtcct
     1021 cgaatcgttc tacggaagga cgatcaccga cgatgcgctc cgcgaggcga tccgcctcaa
     1081 aaaccgtgaa cggcgcgtcc tcaaccgctt cttcgccgtc gcgcggctcg acccgtcccc
     1141 gctgacgggg ctcgaaatcc tcgccgtctc tgactatctg aagtactcca tggcgtcgga
     1201 ggaggcgctc tgcgtggtgg agcagctgac ggcagcgctc gaagaggagc ggcgacgagg
     1261 aaagggaagc caaacggctg gacgcaaacg
//
LOCUS       DAOPKU010000743         1281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_127954_length_1281_cov_5.221860, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000743 DAOPKU010000000
VERSION     DAOPKU010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127954_length_1281_cov_5.221860"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PK849_15460"
     CDS             <1..448
                     /locus_tag="PK849_15460"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPE67580.1"
                     /translation="SKGHSGELIRLHEKKILPCAGCNACKTEGKCILSDDMTALYPLV
                     EEAEVIVHAFPVYWWSMSAQTKLFQDRLYAMDYTHFKGKQLFLLTTYGGEDPNSGPRL
                     CEQSFRDICEFLDMQFRDCIGVCSGMTPVRDNKRALERAFLAGASM"
     gene            514..1266
                     /locus_tag="PK849_15465"
     CDS             514..1266
                     /locus_tag="PK849_15465"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPE67581.1"
                     /translation="MRTDRLRTILLCVCALLLAGGVACASGAPSREELLERPAAGVVA
                     GSNVNVRDRPSTRGKSLGRLGEDGYWEELVVVDAAPGDDGQIWYKILSEKLGEGWMHG
                     RFLRFEGLDDPLGRFFVLLRRDFGTNPAMTQQRLGKPVKIERKTFRPEDYPENVTSVE
                     LRYPGGSVVFWIMKKIPFLVGADFEKGAAFGPISFGDGPEKVERLLGEPHGVRNRVWT
                     YHSGMRRVHIGFGLGPDGEAAVNRLIFERDAF"
BASE COUNT          246 a          380 c          422 g          233 t
ORIGIN      
        1 ttccaaagga cattcggggg agctcatccg gctccacgag aagaagatac tcccgtgcgc
       61 ggggtgcaac gcctgtaaaa cggagggaaa gtgcattctc tccgacgaca tgacagccct
      121 ctaccccctc gtcgaggagg ccgaggtcat cgtccacgca ttcccggtgt actggtggtc
      181 gatgtccgcg cagaccaagc tctttcagga caggctctac gcgatggact acacgcattt
      241 caaggggaaa cagctcttcc tgctgacaac gtacggcggc gaggacccca actccggtcc
      301 gcgcctctgc gaacagtcct tccgcgacat ctgcgaattc ctggacatgc agttccgcga
      361 ctgcatcggc gtctgctccg ggatgacgcc cgtccgagac aacaaacgag ccctcgaaag
      421 ggcgtttctc gcgggcgcgt cgatgtagcg ttccgctgga ggagtttcca gttgcgcccg
      481 ttccacgaag gcgaaaaaaa ggaggaggat acgatgcgca ctgatcgctt acgaaccatc
      541 ctgttgtgtg tctgcgcgct tcttcttgcc ggcggggtcg cgtgtgcatc cggcgcgccc
      601 tcgcgcgagg agctgctcga acggcccgcg gccggagtgg tcgcagggag caacgtcaac
      661 gtccgggacc gcccttcgac caggggaaag agcctcggga ggctgggaga ggacgggtac
      721 tgggaggagc ttgtcgtcgt ggacgccgct ccgggtgatg acggtcagat atggtacaag
      781 atcctctccg agaagctcgg cgaggggtgg atgcacggtc gcttcctccg cttcgagggg
      841 ctcgacgacc cgctggggcg cttctttgtt ctgctccgga gggatttcgg gacgaacccg
      901 gcgatgacgc agcagcggct gggcaaacca gtgaagatcg agcgaaagac atttcgtcca
      961 gaggactacc ccgagaatgt gacgtcggtc gaacttcgct accccggagg gagcgtcgtc
     1021 ttctggatca tgaagaagat tcccttcctg gtaggagcgg acttcgagaa gggcgccgcg
     1081 ttcggcccga tctccttcgg cgacggcccg gagaaggtgg agcgactgct cggcgaaccg
     1141 cacggagtac ggaaccgcgt ctggacgtac cactccggga tgcggcgtgt gcacatcggc
     1201 ttcggtctcg gcccggacgg cgaagcagcg gtgaaccgcc tgatcttcga gcgggacgcg
     1261 ttctgaacag actgtttcat a
//
LOCUS       DAOPKU010000744         1279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_128261_length_1279_cov_5.644608, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000744 DAOPKU010000000
VERSION     DAOPKU010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128261_length_1279_cov_5.644608"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..>1279
                     /locus_tag="PK849_15470"
     CDS             130..>1279
                     /locus_tag="PK849_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67582.1"
                     /translation="MKKGIVARVLVAVLLVLAVVSTGFAADPKYSKSSTFGPMITGGV
                     TDEFLQMVREDPDIKKGKPLRLGKDITDDDLKKVATLADVLTGVLIERNDTITDLSPL
                     SSLSGLTYMKLDGLKGVKTLAPVGALVKMKQLEIRQVEYPDLDFLTGMNDLESLIFFM
                     QPKQIEDISPLKGKAKLKTLRLYGGAGQDGISDISVLEELTELEDLSLYMTKVEDLSP
                     ISGLTKLKRLDLYGIPATDMSPVENLTELRYIWIYATKFEDYSPLANLTKLEEFRAGI
                     SKLDTLEYLESMPKLKIVSMLREDIQDFSPLAKCPNLTEVNLEAVKGVIDLSVFAEHK
                     KLKNLFVRNAEVKNAEAIVALPRLEWLILIDTKGIADVSIFKDLPKLNR"
BASE COUNT          291 a          345 c          381 g          262 t
ORIGIN      
        1 aggttgtctc cgggatgcag gggtctgtga agttcgtgtt ctatctgggg atcgcgctga
       61 tgattggcgg ggcggccgtg gccgtcttct tccggtagcg cgaaacgctc tgcgaggagg
      121 ggaaattcga tgaaaaaggg aatagtggca cgcgttctgg ttgctgtatt gctggtactg
      181 gcagtggttt cgacggggtt tgccgcagac cccaagtact cgaagagctc gacgttcggt
      241 cccatgatta ccggcggtgt gacggacgag ttcctccaga tggttcggga ggacccggac
      301 attaaaaagg ggaagccgct ccgtctcggg aaggacataa ccgacgacga tctgaagaaa
      361 gtcgcgacgc tcgccgatgt gctgacagga gttctgatcg agaggaacga cacgatcacc
      421 gacctttcgc cgctctcctc gctctccggg ctgacctaca tgaagctcga cggcctcaag
      481 ggtgtcaaga cgctcgcgcc ggtcggagcg ctcgtgaaga tgaaacagct tgaaatacgc
      541 caggtcgagt accctgatct ggatttcctt accgggatga acgatctcga atcgctcata
      601 ttctttatgc agcccaaaca gatcgaggac atttcgccgc tgaagggaaa ggcaaagctg
      661 aagacgctgc gcctctacgg aggggccggg caggatggca tatcggacat ctccgtgctg
      721 gaggagctga cggagcttga ggacctcagc ctctacatga ccaaggtgga ggacctctcc
      781 cctatctccg gcctgacgaa gctgaagcgg ttggatctct acggcattcc cgcaaccgac
      841 atgagccctg tcgaaaacct cacggaactc cggtacatct ggatctatgc gacgaagttc
      901 gaagactact cgccgctcgc caacctgacg aagctcgagg agttccgcgc cggaatttca
      961 aagctcgata ctcttgaata cctggagagc atgcccaagc tgaagatcgt ctccatgctc
     1021 cgcgaggaca ttcaggactt ttcgccgctg gcgaagtgcc cgaacctgac cgaggtcaac
     1081 ctggaagctg tgaaaggagt tatcgacctg agcgtcttcg cggaacacaa gaagctgaag
     1141 aatttgttcg tccgcaatgc agaggtcaag aacgccgaag ccatcgtcgc cctgccgagg
     1201 ctggagtggc tgatcctcat cgacacgaag ggtattgccg acgtctctat tttcaaggat
     1261 cttcccaaac ttaacaggg
//
LOCUS       DAOPKU010000745         1279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_128333_length_1279_cov_2.617647, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000745 DAOPKU010000000
VERSION     DAOPKU010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128333_length_1279_cov_2.617647"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PK849_15475"
     CDS             <1..476
                     /locus_tag="PK849_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67583.1"
                     /translation="APWEADSMVLRTSIPAHVKPTPQFLDFLARLKQAPKETAVIRAS
                     DVYDPEIVFFIGGVRADTEGRAHFMQRHPGGVSDYVSYDTVEFSHHVRWILKNKNQKV
                     LGILPSTCEPEGYAAEKKKGNVRSLAGNASVVFRIRAGALDSAQGDVYEKKLQNS"
     gene            504..>1279
                     /gene="rbsK"
                     /locus_tag="PK849_15480"
     CDS             504..>1279
                     /gene="rbsK"
                     /locus_tag="PK849_15480"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPE67584.1"
                     /translation="MNARPKAAVIGSNMIDLVTLIDRMPKLGETVEAPHFDIGFGGKG
                     ANQAVALARLGADVLMVSKVGDDIFGLEVKRNFEENGIDSRYVDVVVGASNGVAPIFV
                     DKDANNSILIIKGANNALSPEDVDRAAEDIKKCNLIILQLEVPLKTVYHAIEFGVKNK
                     IPVILNPAPGAELDFEYVRQADFLVPNETELEIVSRMPVGTMDDIVAAARSLVEKGAR
                     NVIVTLGAKGSLLVNGKEEVLVEPFAVKSMDSTGAGDAFIG"
BASE COUNT          300 a          311 c          389 g          279 t
ORIGIN      
        1 ccgctccctg ggaggcggac tccatggtct tgcggaccag catccccgcg catgtgaagc
       61 ccactccaca gtttttggat tttcttgccc gtctgaagca ggctcccaag gaaaccgccg
      121 tcattcgcgc ttcggatgtg tacgatccgg aaatcgtctt ctttatcggc ggcgttcggg
      181 cggatacgga ggggagagct catttcatgc aacgtcaccc gggaggtgtc tcggactacg
      241 tttcttacga cactgtggaa ttttctcatc atgtccggtg gatactgaag aacaaaaatc
      301 agaaggtcct tggtatcctc ccttcgacgt gcgaaccgga ggggtatgcc gcggagaaaa
      361 aaaaggggaa tgtccgttct ctggcgggaa acgcatcggt tgttttccgt atccgggcgg
      421 gggctctgga cagcgctcaa ggcgatgtgt acgaaaaaaa gctacagaat tcctgagata
      481 cagataagac agggggaaaa ggaatgaacg cacgaccgaa agcggctgtc atcgggagta
      541 atatgatcga cttggttacc cttatcgaca ggatgcccaa acttggagag accgtcgagg
      601 ctcctcattt cgacatcgga tttggaggaa agggcgcgaa ccaggcggtg gcgctggcca
      661 gactgggggc ggatgtgctg atggtctcga aagtgggaga cgatatcttc ggccttgagg
      721 tgaagcgcaa tttcgaggag aacggcatcg actccaggta tgtcgatgtc gtcgtcgggg
      781 cttcgaacgg cgtggcgccg atcttcgtgg acaaggatgc caacaacagc attcttatca
      841 taaaaggggc gaacaacgcg ctctctccgg aggatgtgga ccgtgccgcg gaggatataa
      901 agaagtgcaa cctgatcatt ttgcagcttg aggtgccgtt gaaaacggtc tatcacgcga
      961 tcgaattcgg cgtgaaaaac aaaattcctg tgatattgaa ccctgcgccc ggggcggaac
     1021 tcgatttcga atatgtacgg caggcggatt ttctcgtccc taacgaaacc gaactcgaga
     1081 ttgtctccag aatgcctgtc gggacgatgg acgatattgt cgccgctgcg cgaagtcttg
     1141 tcgaaaaggg cgcccgcaat gtcatcgtga cgctcggcgc caagggctcg cttcttgtga
     1201 acgggaaaga ggaagttctt gtcgagcctt tcgcggtaaa atccatggat tccacaggag
     1261 cgggcgatgc ctttatagg
//
LOCUS       DAOPKU010000746         1272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_129362_length_1272_cov_2.755136, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000746 DAOPKU010000000
VERSION     DAOPKU010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129362_length_1272_cov_2.755136"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PK849_15485"
     CDS             complement(<1..522)
                     /locus_tag="PK849_15485"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HPE67585.1"
                     /translation="MKIELRPLAEIKPYERNPRDNDAAVAAVAESIQRFGFRQPFVVD
                     GEGVIVCGHTRWKAAQKLGLEKVPVHVATDLTPEQIRAYRIADNKTNELASWNMELLP
                     LELGELQGAGIDWSLLGFDQDELAKLLGGEIKAGLTDPDDIPEPPDEAVTQPGDLWIL
                     GEHRLLCGDSGSVD"
     gene            complement(519..656)
                     /locus_tag="PK849_15490"
     CDS             complement(519..656)
                     /locus_tag="PK849_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67586.1"
                     /translation="MIADIVGTILTHSLCYLGGIYTVFIAWVLWPQGKKDAAVVEEPT
                     S"
BASE COUNT          236 a          428 c          358 g          250 t
ORIGIN      
        1 gtcgacagac ccgctgtcac cgcaaagcaa gcggtgctca ccgaggatcc acaggtcgcc
       61 cggttgcgtc accgcctcgt ccggtggctc agggatgtca tcggggtccg ttagtcccgc
      121 cttgatctcg ccgccgagga gcttcgcgag ctcatcctgg tcgaacccga gcagcgacca
      181 gtcgatgccg gcgccctgca gttcgccgag ttcgagcggc aacagctcca tgttccagga
      241 ggccagctcg ttcgtcttgt tgtcggcgat gcggtacgca cggatctgct caggcgtgag
      301 gtcggtcgcc acgtgcaccg gcaccttctc gaggccgagc ttctgcgccg ccttccagcg
      361 cgtgtggccg cagacgatga cgccctcgcc gtccaccacg aacggctggc ggaagccgaa
      421 ccgctggatc gactcggcca ccgccgcgac ggctgcatcg ttgtcgcggg gattgcgttc
      481 gtacggtttg atttcggcca gcggcctcag ttcgattttc atgacgtcgg ctcctccacg
      541 acggccgcat ccttcttccc ctgcggccac aggacccagg cgatgaacac cgtgtagatc
      601 ccgcccaagt agcacagcga atgcgtcaaa atggttccga caatgtctgc gatcacagca
      661 agctctcctt tcgctggggc aaagcagccc cgaggtttgc gactcgatac ggctgcgctg
      721 caaggcgcct gtagccccca cagcggccac cggtgcccca gtggccaacg gcaccgccgg
      781 cgccgccgcg tggccacgga acgccacggt tcgcgttctg cgccaacgtg gcgcgccgcg
      841 cacaaccgcg accggacagc cgaaacaaac tcagactatg cttgcggctg ttcccgccgc
      901 cctttggcac ggaaattgcc agggaagtac ctaatcgcga attgccaatc gacttctttc
      961 ccttctttcc tctttcaccc ctccctctca cgcgtgcgca cacacagaca ggagaacaga
     1021 gaaaagaagt gagagaggaa tagagagaaa gagagtaata agaagagaag tagtgttttt
     1081 tactatatat attcagcatt tcttccttca cccttccttc tcattcttcc cgctgtttcc
     1141 ctcagccagc agctcgtaga ccaagccggt acggcccggc gtcgtcaccg gagtcatggc
     1201 gatgtccccc cgctgccgga gcgtgtcgat aatctcgcgg aagctcttcg cgtcgatctt
     1261 catccgtttg ag
//
LOCUS       DAOPKU010000747         1269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_129793_length_1269_cov_4.011532, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000747 DAOPKU010000000
VERSION     DAOPKU010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129793_length_1269_cov_4.011532"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..>1269
                     /locus_tag="PK849_15495"
     CDS             147..>1269
                     /locus_tag="PK849_15495"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPE67587.1"
                     /translation="MAKNAGKIDIEMHKGTSNLTSFGGLCSVIKLFFKMGLDKSIDAL
                     IGARNERGAKDSEHVLSLVLLNLGGGTAVDNLNFLREKLAFEKFGIRIPSPSAGREWL
                     KSFHNPSEDSKRGMGQAFIPEENKYLSGWRDVFAKSFHFAWKLNPRAFLTLDMDDTEI
                     ETGVEGSLFNYKGNRGFWAFITYCAELGQIVSTRYCDGNVPPGWRQLEEFRNVLGTVP
                     EGVKEVALRSDSAGYQAELLRFCNENPEKRFRRIHYGVSADVNDEFRAAAKAVSETEW
                     KPLKTFEDKNGKIVVLQEWAEVAYAPNSLSTKKHGPDYRFFAVREKWDGSGKDTEKEE
                     SPEKPVAGWEGHGEQLYLEGAIETLEAKSDKVRKLHLTQF"
BASE COUNT          318 a          311 c          406 g          234 t
ORIGIN      
        1 taggtttcga tagacgtaaa tgtgagtagc gaagaagacc caaatccgca ggcagaaaaa
       61 ataaaaaccc ctctggactg ttcgtagaac agtgataagg tagtgtttgc gaagacacag
      121 accttcacca aaggggtgca tctattatgg caaagaacgc gggaaaaatc gacatcgaaa
      181 tgcacaaagg cacgagcaat cttacatctt ttggagggct gtgctccgta atcaaacttt
      241 tcttcaaaat gggactcgac aagtcgatcg acgcgttgat aggagcgagg aacgagcgtg
      301 gcgcgaagga ctccgagcat gttctgtccc tcgtgcttct caacctgggc ggagggacgg
      361 ccgtggacaa tctgaacttt cttcgcgaga agctggcctt cgagaaattc ggcatccgca
      421 ttccttctcc gagcgcaggc agagagtggc tcaagtcttt ccacaacccc tcggaagact
      481 cgaagagagg gatgggacaa gccttcatcc ccgaggagaa caagtatctg agcggttggc
      541 gggacgtctt cgcgaagtcg tttcatttcg cgtggaagct gaaccctcgc gcctttctca
      601 cgttagatat ggacgacacg gagatagaga cgggagtgga gggctctctg ttcaactaca
      661 aggggaacag aggattctgg gccttcataa cctactgcgc ggaacttggt cagatcgtct
      721 cgacgagata ctgcgacggg aacgtgcctc cggggtggag gcagcttgag gagttccgga
      781 atgtgctcgg gacggttccc gagggagtga aggaagtggc gcttcggagc gacagcgccg
      841 gataccaggc ggaactgctc cgtttctgca acgagaatcc ggagaagcgc ttcaggcgga
      901 tccactacgg agtctccgcg gacgttaacg acgagttcag agcagcggcg aaggccgttt
      961 ccgagacgga gtggaaaccg ctgaagacgt tcgaggacaa gaacgggaag atcgtcgttc
     1021 tccaggagtg ggcggaggta gcgtacgcgc ccaacagtct ctcgacgaag aagcacggtc
     1081 cggactaccg gttcttcgcc gtccgggaga agtgggacgg ttccgggaag gatacggaga
     1141 aggaggagtc tccggagaag ccggtcgcgg ggtgggaagg ccatggcgag cagctctacc
     1201 tcgagggggc catcgagact ctcgaagcga agagcgacaa ggtgaggaag ctccacctca
     1261 cccagttcg
//
LOCUS       DAOPKU010000748         1263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_130695_length_1263_cov_4.913907, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000748 DAOPKU010000000
VERSION     DAOPKU010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130695_length_1263_cov_4.913907"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PK849_15500"
     CDS             complement(<1..663)
                     /locus_tag="PK849_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HPE67588.1"
                     /translation="MRGLFRFNEEFVYKMPVHFGGDPFYPVRTVYGDMTCLGVSFRTE
                     EDALLRFIPEDFEMLEPVVNVQYANCRDVDWMAGGEYRLIQASVPVRYEGNSEGLVGE
                     YVLVIWENKACPIIGGREEDGMPKIFADIASERHVGERWFTSASYESNTFLSLEVTRR
                     GELDEEQIVEVNKNPKINFFGWRYLPNLGKGGATLSHATLYPQEMVVRQVWTGEGSLR
                     WTP"
     gene            complement(780..>1263)
                     /locus_tag="PK849_15505"
     CDS             complement(780..>1263)
                     /locus_tag="PK849_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HPE67589.1"
                     /translation="LGELSTYGIMKDESERSLRAKIDYLLSRGYISLTDDEFPVVKTT
                     PLAKGVLFRGEKLAMPVAKQEPKPLAAEPKGVSMLMEPTDLELFDKLRALRKEIAGAQ
                     GVPAFVVFTDATLRDMCEKLPQTPDELLQVSGVGYAKREKYGAAFLELLREHSTASVP
                     "
BASE COUNT          248 a          407 c          339 g          269 t
ORIGIN      
        1 cggcgtccag cggagcgatc cctcgcccgt ccatacctgg cggacgacca tctcctgcgg
       61 atagagcgtc gcatggctca gcgtcgcgcc tcccttgccg aggttcggca ggtatcgcca
      121 gccgaagaag ttgatcttcg ggttcttgtt gacctcaacg atctgctctt cgtcgagttc
      181 cccccggcgt gtgacttcga gtgagaggaa ggtgttgctc tcgtagcttg cggaggtgaa
      241 ccagcgctcg cctacgtgac gttccgaggc gatgtccgcg aagatcttcg gcatgccgtc
      301 ctcctcgcgc ccgccgatga tcgggcacgc cttgttctcc cagatgacca gcacgtactc
      361 gccgacgagt ccttcggaat ttccttcgta ccgtaccggg acgctcgcct gaatcagccg
      421 gtactcgccc ccggccatcc agtcgacatc gcggcagttc gcatactgga cgttgaccac
      481 cggttcgagc atctcgaaat cctcggggat gaagcgcagc agcgcgtcct cctccgtccg
      541 gaacgaaacg ccgagacacg tcatgtcgcc gtagaccgtc cgcaccgggt agaacggatc
      601 gcctccgaaa tgcacaggca tcttgtagac gaactcttcg ttgaacctga acagccctcg
      661 catacgcacc cctccattcg aagaatatgt tcgtctatta tactcttttt actacaacaa
      721 aaagagcgca tccccaaaaa aggacacgct cttcacacac aaaatctgtg attctgtttt
      781 tacggaacgg aagcagtcga gtgttcgcga agcagttcca aaaaggccgc gccgtacttt
      841 tcccgtttgg cgtaccccac gccgctcacc tggagcagtt cgtccggcgt ctgcggcagc
      901 ttctcgcaca tgtcgcgaag cgtcgcgtcg gtaaagacca cgaaggcggg aacgccctgc
      961 gcgccggcga tctctttccg gagggcgcgc agcttgtcga agagttcgag gtcagtcggc
     1021 tccatgagca tgctcacacc tttcggctcg gcggcaagcg gtttcggctc ctgcttcgcc
     1081 acgggcatcg cgagcttctc tccccgaaag agcactccct ttgccaacgg agtggtcttc
     1141 accacgggaa attcgtcgtc cgtgagcgaa atataccctc gcgaaagcag atagtctatc
     1201 tttgcgcgca acgaacgctc gctctcatcc ttcattatgc cgtaggttga aagttcgcca
     1261 agg
//
LOCUS       DAOPKU010000749         1260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_131187_length_1260_cov_4.036515, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000749 DAOPKU010000000
VERSION     DAOPKU010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131187_length_1260_cov_4.036515"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1260
                     /locus_tag="PK849_15510"
     CDS             <1..>1260
                     /locus_tag="PK849_15510"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPE67590.1"
                     /translation="LKYLEGLEQDRADEALLSGKPYAYILDAPYRWESWAAPKGKDGQ
                     LDRNRAMTGDDLRDFVNQKLFPYLHGFKSRATGPNTVEYKIGEIFGEIKNKIQSGYNL
                     REIIDYVDELKFRSQVEKHELSALYEEKIQRMGNAGRNGGEYYTPRPLIRAIVEVVDP
                     KIGERVYDGACGSAGFLCEAHVHMKKRNPRRTTAQDRTLQESTFYGKEKKSLAYVIAI
                     MNMILHGIEAPNIIHTNTLSENLADVQEKDRYHVVLANPPFGGKERPEVQKNFPIRTS
                     ETAYLFLQHFIRMLKAGGRGGVVIKNTFLSNTDNGAVSLRKHLLESCTLHTVLDCPGG
                     TFQGAGVKTVVLFFEKGSPTKKIWYYQLDPGRNLGKTNPLNDGDLAEFLELRKTFADS
                     PKSWSVDAGGIDKETFDLSVKNPNNGT"
BASE COUNT          304 a          373 c          369 g          214 t
ORIGIN      
        1 ttttgaaata cctggagggg ctggaacagg acagggcgga cgaggccctc ttgtccggga
       61 agccgtacgc atatattctc gacgccccct atcgctggga atcgtgggcg gccccgaaag
      121 ggaaggacgg gcagctcgac cgcaaccggg ccatgaccgg agacgatctg cgagacttcg
      181 tgaaccagaa gctctttccc tatctgcacg gattcaaaag ccgcgccacc ggtcccaaca
      241 cggtggagta caagatcggc gagatcttcg gcgagatcaa gaataaaatt cagagcggct
      301 acaatctcag ggagatcatc gactacgtcg acgaattgaa gttccgctcc caagtggaaa
      361 agcacgaact ctcggcgctg tacgaagaaa agatccagcg catggggaat gccggtcgca
      421 acggcggcga gtactacacg ccgcgtccgc tgatccgcgc catcgtcgaa gtagtcgacc
      481 ccaagatcgg agagcgcgtc tatgacggcg cctgcggttc cgcgggtttc ctgtgcgaag
      541 cgcacgttca tatgaagaag cgcaatccgc gccgcaccac ggcccaggac agaactcttc
      601 aggagagcac tttctacggc aaggagaaga agtccctcgc ctacgtcatc gccatcatga
      661 acatgattct gcacggcatc gaagcgccga atatcatcca caccaacacg ctctccgaga
      721 acctcgccga tgtacaggag aaggaccgtt atcacgtcgt gctggccaat cctccattcg
      781 gcggcaaaga gcgtccggag gtacaaaaga acttccctat ccggacgagc gagacggcgt
      841 accttttcct ccagcacttc atccggatgc tcaaggcggg aggacgcggc ggcgttgtga
      901 tcaagaacac ctttctctcc aacacggaca acggcgccgt cagcctccgg aagcatcttc
      961 tggagagctg caccctgcac accgttctgg actgtccggg cggaaccttc caaggggccg
     1021 gggtgaagac ggtggtgctc ttcttcgaga agggatctcc gacgaagaag atatggtact
     1081 accagctcga cccgggccgg aacctcggaa agaccaatcc gctgaatgac ggcgaccttg
     1141 cggagttcct cgagctgcgg aagactttcg ccgactcgcc caagagttgg agcgtggacg
     1201 ccgggggcat cgacaaggag acgttcgatc tctcggtgaa gaatccgaac aacggaacgg
//
LOCUS       DAOPKU010000750         1258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_131651_length_1258_cov_1.532003, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000750 DAOPKU010000000
VERSION     DAOPKU010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131651_length_1258_cov_1.532003"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1258)
                     /gene="pglZ"
                     /locus_tag="PK849_15515"
     CDS             complement(<1..>1258)
                     /gene="pglZ"
                     /locus_tag="PK849_15515"
                     /inference="COORDINATES: protein motif:HMM:NF033450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type B"
                     /protein_id="HPE67591.1"
                     /translation="TVPVFYTPGFGRQDLRVVEGNPNELKPLAELQFRGTFWSQSNGK
                     DWTVSAFLKSEKGLALDVARDGDSKRAMLLSLHRLLNEELEMLEGKRLDRDFFNTLLT
                     GDPVRDLLFWLDRGEDFRKSRGANEWRAFVEVCGAQFGFNPQKEGELSGAERLASRRG
                     SWANVWERFCEAPKRYPGIPVLLRTCSPPKENIFWKLNDGTFDGWPQWNEDMERVLRE
                     ELLSLADLPPHEAREKVAALEMKHARRRTVVWTELGEAPLASALEFIAILAEITSKPL
                     AAGTLEDLESAYRSRGWKADDAMLRALAFGDTLADSAVIESAVRSLYLPWAEESARSL
                     QAMAESRGYPGDKALSAHSTSSEEGECVLFVDGLRLDTAKRLAGMLQAVGFEVGESPA
                     WAALPSVTATGKAAVSPVRDKIAGSDL"
BASE COUNT          241 a          427 c          363 g          227 t
ORIGIN      
        1 agatcgctgc cggcgatctt gtcgcgcacc ggagagactg ccgctttacc ggtcgctgtc
       61 acgctcggga gagcggccca cgcgggcgac tccccgacct cgaatccgac ggcctgaagc
      121 atccccgcga ggcgcttcgc cgtatccagc cgaagaccgt cgacgaagag aacgcactcg
      181 ccttcttcgg aggaagtgga atgcgcggag agcgccttgt ctcccggata gccccggctc
      241 tccgccatcg cctgcagaga gcgggccgac tcctccgccc acgggagata gagcgaacgg
      301 acagcgctct ctatcacggc ggaatccgca agagtatccc cgaatgccaa ggcacgcagc
      361 atcgcgtcgt ccgctttcca tccgcggctt ctgtacgccg actccaaatc ctcaagagtg
      421 cctgcggcaa gaggtttcga ggtgatttcc gccagaatcg ctatgaattc aagcgcggaa
      481 gcgagagggg cctcccccag ctccgtccag acgacggtcc gccgccgtgc gtgcttcatt
      541 tcaagcgcgg cgaccttctc ccttgcttca tgggggggca gatccgcaag cgagagcagt
      601 tcttcccgca gcaccctctc catatcctcg ttccactggg gccagccgtc gaacgttccg
      661 tcgttcagct tccagaagat gttttccttc ggcggcgaac aggttcgcaa caggacgggg
      721 atgccgggat agcgcttcgg cgcctcgcag aaacgctccc agacgttcgc ccacgatccc
      781 cgacgcgagg caagcctttc agcccccgaa agctccccct ccttctgcgg gttgaaaccg
      841 aactgcgcgc cgcagacctc gacgaaggcg cgccattcgt tcgctccccg gctcttcctg
      901 aagtcctctc cgcggtcgag ccagaagaga agatcgcgca caggatctcc cgtgagcagc
      961 gtattgaaga agtcccggtc gaggcgcttt ccctcaagca tctcaagttc ctcgttcagc
     1021 aagcgatgaa gagagagcag catcgcgcgc ttggaatctc cgtccctggc cacgtcgagc
     1081 gcgagcccct tttccgactt gagaaaagcc gaaacggtcc agtccttccc gttgctctga
     1141 gaccagaagg ttccccgaaa ctgcagttca gcaagcggtt tgagctcgtt cggatttccc
     1201 tcgacgacgc gcagatcctg acggccgaat ccgggggtgt agaagacggg gacggttc
//
LOCUS       DAOPKU010000751         1256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_131850_length_1256_cov_4.070774, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000751 DAOPKU010000000
VERSION     DAOPKU010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131850_length_1256_cov_4.070774"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /gene="thpR"
                     /locus_tag="PK849_15520"
     CDS             <1..440
                     /gene="thpR"
                     /locus_tag="PK849_15520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPE67592.1"
                     /translation="KFLGEQEQRLLEDLMRSFEKRIASSGQDVIALSSSALGAFPNIR
                     TARTLFAELCGDLVPLKAIASIVEDVSAEHGIPREKRPFKPHITLARTRRTETVIISP
                     KDIPLFSKIEWQATSVTLMKSSLTPAGPIYTPLKIWEFPAKVF"
     gene            500..>1256
                     /gene="recA"
                     /locus_tag="PK849_15525"
     CDS             500..>1256
                     /gene="recA"
                     /locus_tag="PK849_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPE67593.1"
                     /translation="MAKKKTPQTRDDVLELAIEDIRSKFGEGSIMRLGDSFKAAVEVI
                     STGILPLDVALGIGGLPRGRIVEIFGPEGSGKTTVALHAVAEAQKAGGVAAFIDAEHA
                     LDPRLAHNLGVDVQSLYIAQPDSGEQALYVLDTLVRSSAVDIVVIDSVAALTPQAEID
                     GKIGDSQVGLQARLMSYALRRLTSAISKSNCVVVFINQLRAKISTGYSQGPQETTTGG
                     RALKFYSSVRIEVKRGKGVSKGDDTNGHELWMKV"
BASE COUNT          324 a          296 c          322 g          314 t
ORIGIN      
        1 tcaagtttct cggcgaacag gagcaacggc tgctcgagga tcttatgcga tcatttgaga
       61 aaagaattgc ttcttccggg caagatgtga tagctctttc ctcttccgct ctcggtgctt
      121 tcccaaatat acgaacggcc cgaactcttt ttgccgaact gtgcggcgat cttgtcccgc
      181 tgaaagctat tgcgtcaatt gttgaagatg tgagtgcgga acatggaatt ccacgagaaa
      241 aaagaccttt caagcctcac ataacacttg cgagaacgcg ccgtacggaa acagtgataa
      301 tatccccaaa agatatccct cttttcagta aaatagagtg gcaagcgaca tcggtaacgt
      361 tgatgaaaag ttctctgaca cctgccggcc caatctatac ccctctaaaa atctgggaat
      421 tcccggcgaa agttttttga taggaacacg acttttcctg tacaatacta ctgttcatat
      481 tctggaagga ggaatttgtt tggcgaagaa gaaaactcct cagacgcgtg acgacgttct
      541 tgaacttgcc attgaggata tccgcagcaa atttggagaa ggctccataa tgcgtctcgg
      601 agattccttt aaagcggctg ttgaggttat ttcaacaggt attctccctt tggatgtggc
      661 tctgggtatc ggtggccttc cccgtggtcg tatcgttgaa attttcgggc ctgaaggctc
      721 cgggaaaaca accgtagctc tgcatgccgt tgctgaagcg cagaaagccg gaggagttgc
      781 cgcctttatt gacgctgaac atgctctgga cccgagactt gcgcataatc tcggcgttga
      841 cgtgcagtcc ttatatatcg cacagcccga cagcggagaa caggctcttt acgttttgga
      901 cacgctggtt cgcagcagcg ccgttgatat tgtggttatc gattcagtcg ctgctctgac
      961 cccgcaggcg gaaatagacg gtaagatcgg agatagccag gtaggccttc aggctcgtct
     1021 tatgtcctat gctctcagac gactgacctc agcaatttcg aaaagcaact gcgtcgtggt
     1081 ttttataaac cagttgcgcg caaaaatcag cacaggctat tcgcaggggc ctcaagaaac
     1141 gacaaccgga gggcgcgctc tcaaatttta tagctctgtg cggatagaag taaaacgggg
     1201 gaaaggcgtt agtaaaggag acgacacaaa cgggcatgaa ctatggatga aggttg
//
LOCUS       DAOPKU010000752         1249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_132991_length_1249_cov_2.563652, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000752 DAOPKU010000000
VERSION     DAOPKU010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132991_length_1249_cov_2.563652"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..>1249
                     /locus_tag="PK849_15530"
     CDS             398..>1249
                     /locus_tag="PK849_15530"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPE67594.1"
                     /translation="MAEKDNAFRRMLGDKELFLRFLRRFLRRDMPLIVDALIESKGFS
                     PEDVVLENISFIPPDLREKRSDVVYRIRKGSLEAYVYILIEHQSRVDFLMPYRLLSYM
                     VQLWSRCIEDAGELARRKSFLLPPIVPVVFYEGEDAWTAAKYFTEKVRGADSFRGYVP
                     EFEYRLISLRDEDSEELLAKPDALSSLMYLAQPFKSEDFFEVSERIRNLLLVFPEEEK
                     ILLAKHLGGYLKILAKKEGVEIEGAPDEYFEQKGAEGMLTYLEKEIRRYKKQGREEGQ
                     LEKGISVA"
BASE COUNT          286 a          276 c          384 g          303 t
ORIGIN      
        1 ggctgcgaaa ttttccaatg attgccggcg gaaaggtgtt caggggtctc atatcgactt
       61 tttaatatgt tcggttgcgt ttaaaaacgt ttggaagatt tttacctcag accaagactt
      121 tttaagatac caacaatatc ttcctctcga gttgtatagc ataggcgagt agcgtgagag
      181 tcttaactct cacggactcg caaaccctcg tctgctccga cgacaacgcc gaacgcctct
      241 cgcgcgcgta cgacggcgcg gaggaatttt gggggggcga aagccgaggt caccgcgctc
      301 cttgtgtcaa cccactccct gcaggacctc cgcctgaagt cgcagttcgg tgagcatgct
      361 ataataatca aaaatcttag gtcggaaggg gaatccgatg gcggaaaagg acaatgcgtt
      421 tcggaggatg ctcggcgata aagagctctt ccttcgcttt cttcgccgct ttttgaggcg
      481 ggacatgccg ttgatcgtcg acgctctgat cgaatccaag gggttttcgc ccgaggatgt
      541 tgtgctggag aacatctctt ttattccgcc ggatctgagg gagaagcgct ccgacgtggt
      601 gtaccggata cgtaagggct ctcttgaggc gtatgtctac atcctgatcg agcatcagtc
      661 gagagtggat tttttgatgc cgtaccgctt gctgtcgtat atggtgcagt tgtggtcgcg
      721 ctgtatcgaa gacgcgggcg agttggcgag acggaaaagt tttctcttgc cgcccatcgt
      781 tccggtggtt ttctacgagg gcgaggatgc atggacggca gcgaaatatt tcactgaaaa
      841 agtaagggga gcggactctt tccgggggta tgttccggag ttcgagtatc gcctgatttc
      901 gttgcgggac gaggattcgg aggagctttt ggcgaagccc gatgctttga gcagcttgat
      961 gtatttggcg cagccgttca agagcgagga ctttttcgag gtttccgaac gaattcgaaa
     1021 cctcttgctc gtcttcccgg aagaggaaaa aatcctgttg gcgaagcacc tgggagggta
     1081 cttgaagata ttggcgaaaa aagagggcgt ggaaatcgaa ggcgctccgg acgaatactt
     1141 tgaacagaag ggggctgagg ggatgctgac ctatctggag aaggagatac gaaggtacaa
     1201 gaagcaaggc cgggaggagg ggcagctgga gaaaggcatt tcggtagcc
//
LOCUS       DAOPKU010000753         1248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_133143_length_1248_cov_2.937133, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000753 DAOPKU010000000
VERSION     DAOPKU010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133143_length_1248_cov_2.937133"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PK849_15535"
     CDS             complement(<1..1129)
                     /locus_tag="PK849_15535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPE67595.1"
                     /translation="MKFAEMYADIKPSGMLKLFQAAAATPDVVNLGEGEPDFDTEHDI
                     VDAAARAAKEGYTHYGPIQGYPDVRQAVCNYWERRYGLSSTPDEVMIMAGGVQSVHLA
                     LQALLNPGDEVIVAEPCFSPYFEQIYQHRGVAVHLPTTEAQGFVPSGEDIERLITPRS
                     RVLMICSPSNPTGRVMQRRQMESIARAAEKHDLVVLSDEIYDSMVYTGKHVPFATVPG
                     MKERTLTMGGLSKSHCMTGWRIGYAIGPAPLIKVMSLIGANQTYGVNVPTQRAALYAL
                     EHHDAKMEERARVFRDRLAYVAKRLNAMKGVRCAEPEGAFYLFPDIRGTGADSEQFAW
                     GLLEKGHVATLPGSAFGACGEGYVRIACTRSMEMLEKGMDRM"
BASE COUNT          178 a          413 c          422 g          235 t
ORIGIN      
        1 ccatccggtc cattcccttt tccagcatct ccatcgagcg ggtgcaggcg atgcggacgt
       61 acccctcgcc gcaggcgccg aaggccgatc cggggagggt ggcgacgtgc cccttctcca
      121 gcagccccca cgcgaactgt tcgctgtccg cgcccgtgcc gcggatgtcg gggaagaggt
      181 agaaggcccc ctccggctcg gcgcagcgga cgcccttcat cgcgttgagc cgcttggcga
      241 cataggcgag gcggtcgcgg aagacgcggg cgcgctcctc catcttcgcg tcgtgatgtt
      301 cgagcgcgta gagggcggcg cgctgggtgg gcacgttgac gccgtaggtc tggttcgccc
      361 cgatgagcga catgaccttg atcagcggcg cggggccgat ggcgtacccg atgcgccacc
      421 ccgtcatgca gtggctcttc gagaggccgc ccatggtgag ggtgcgctcc ttcatgccgg
      481 ggacggtggc gaaggggacg tgcttcccgg tgtagaccat ggagtcgtag atttcgtcgg
      541 agaggacgac gaggtcgtgc ttctccgcgg cgcgggcgat ggactccatc tgtctgcgct
      601 gcatcacgcg cccggtgggg ttgctcggtg agcagatcat cagcacgcgc gagcgcggcg
      661 tgatcagccg ttcgatatcc tcccccgacg ggacgaaccc ctgcgcctcg gtggtgggca
      721 gatggaccgc gacgccccgg tgctggtaaa tctgctcgaa gtagggggag aagcagggct
      781 ccgcgacgat cacctcgtcc cccgggttca gcagcgcctg aagcgcgagg tggaccgact
      841 ggacgccccc ggccatgatc atgacctcgt cgggggtgga cgacaggccg tacctgcgct
      901 cccagtagtt gcagacggcc tgccgcacgt ccgggtaccc ctggatgggg ccgtagtggg
      961 tgtacccctc tttggcggcg cgggccgccg cgtcgacgat gtcgtgctcc gtgtcgaaat
     1021 ccggctctcc ctctcccagg ttgaccacgt ccggagtggc cgctgccgcc tggaagagct
     1081 tcagcatccc cgacggcttg atgtcggcat acatttcggc gaacttcata ctatcctctc
     1141 ctcgttccgt ttcgcgctac tcgtactcct tgagcatggc tgcgtactcc tcttccatgc
     1201 ccttccgcac gtggtagcag tgctcgtcgc cggggaactt cccggtat
//
LOCUS       DAOPKU010000754         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_134178_length_1241_cov_4.528668, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000754 DAOPKU010000000
VERSION     DAOPKU010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134178_length_1241_cov_4.528668"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK849_15540"
     CDS             <1..208
                     /locus_tag="PK849_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPE67596.1"
                     /translation="AGAGGEIQLTDGIRTLISQEPVWGVVYKGRRFDCGTQPGWLHAN
                     IRLAMEHPALKEVVLRAVKEYEEE"
     gene            428..>1241
                     /locus_tag="PK849_15545"
     CDS             428..>1241
                     /locus_tag="PK849_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HPE67597.1"
                     /translation="MCGIVGYIGHRNVADVLVKGLQSLEYRGYDSAGIAVINYPDEEQ
                     TLRTPAVHIVKGVGKVAELKSMLAAHPVSGHQGIGHTRWATHGGVTETNAHPHADLSS
                     RLVLVHNGIVENYRELRAELLVPEEQYKSETDTEVIAQLLSALYTGGGAQDMINALVA
                     LHGRLRGSFALVIMVKDIPGKIYCLRKGSPLVVGFSEEGEALCASDVPAILPYTQDVV
                     YLDDGDIAELSAEGMRFWSAAGESLEKSEVHIDWDVSMVDKGGYSHYMLKEIN"
BASE COUNT          279 a          329 c          388 g          245 t
ORIGIN      
        1 ggcgggagcg ggaggagaga ttcagctgac cgacggcatt cgaacgctga tcagtcaaga
       61 gcctgtctgg ggcgttgtgt acaagggaag gcgtttcgac tgcgggacgc agccgggatg
      121 gctgcacgcc aacattcgcc ttgccatgga gcaccctgca ttgaaggagg tggtgctgcg
      181 agcggtgaag gaatacgaag aagagtagac agcgcatgaa cgaagcgcct gcgccggaga
      241 acagctcccc ggatccgggg aatcgagtct tcggctgacg aggacggagg agtatcgatc
      301 tgtcggcgga tgcctccggg gccgtgagag accggcccgc aaagcctttc tgcaaaaccg
      361 gaaagtaatt tccgggacaa agtgaaatgc agtctctcgt gagtggatat aaaggcggtg
      421 ctgtataatg tgcggaatcg ttggctatat tggccaccga aatgttgcgg atgtacttgt
      481 aaaaggcctt cagtcccttg aatacagagg gtatgactcg gcggggatcg ccgttatcaa
      541 ctaccctgac gaagaacaaa ctctccggac gcccgcggtg catatcgtca agggcgtcgg
      601 caaagtggcc gagctgaagt ccatgctcgc cgctcatccc gtttccggcc atcaggggat
      661 agggcatacc cggtgggcaa cccacggggg cgtcaccgaa accaacgccc atcctcacgc
      721 agatctttcc tccaggttgg tgcttgtcca taacggaata gtggagaact accgtgaact
      781 tcgcgccgag cttcttgttc ccgaggaaca gtacaagtcg gagaccgaca cggaggtcat
      841 cgcgcagctt ctctccgccc tttataccgg cggcggagca caggacatga tcaatgccct
      901 cgtcgccctc cacggacgct tgagaggttc gtttgcgctt gtcatcatgg tgaaagacat
      961 tcccgggaag atctactgcc ttcgcaaagg ctcgccgctg gtcgtcggct tttcggaaga
     1021 aggggaagcg ctctgcgctt cggatgtgcc cgccatactt ccgtatacgc aggacgtcgt
     1081 ctatcttgac gacggggata tcgccgaact ctcggctgaa ggaatgcgtt tctggagcgc
     1141 cgcgggggaa tcgctcgaaa agagtgaagt gcatatcgac tgggatgtct ccatggtgga
     1201 caaggggggc tactcccact atatgctcaa agagatcaac g
//
LOCUS       DAOPKU010000755         1236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_135023_length_1236_cov_3.720576, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000755 DAOPKU010000000
VERSION     DAOPKU010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135023_length_1236_cov_3.720576"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PK849_15550"
     CDS             complement(<1..926)
                     /locus_tag="PK849_15550"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HPE67598.1"
                     /translation="MKTGFLLNRISSSKRSGVVLLSVLLISVFLVSASTGFAIFARRT
                     MRRFDREQRIFTARMVSEVAMDAAKLLLSKHNGKSHSAEDEFFAPRTFDFPDAGVSVT
                     MRMEPLDDRIPLNNLFLPDGKTLRREMEAPWSALWQKVGAEQLENRVLDFLDEDTEPR
                     LGGGERTGNLNRKLLSLEELLLVPDMKPETLSGFGGKPGVRALATIWSSGKINMNVAP
                     VEVLSLLEGLDEKLAAEIVKSRTEKPLESIVDLTRNGGFPPDAVPKLMNIATFKSDWF
                     RVSFDVVFGDGEMIATRGILKKTLKSWNTVQW"
     gene            complement(901..>1236)
                     /locus_tag="PK849_15555"
     CDS             complement(901..>1236)
                     /locus_tag="PK849_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67599.1"
                     /translation="DDRLAFWSDRGGEPGVRAVRRFLPSAGKIGEPGVYLWILPFLTP
                     GSVNWDMLEPEKGKLLFSEAESLRFALLPFDQNEWSDEYKGNRPRGIRITVKIGEEEF
                     VHEDWLPPQ"
BASE COUNT          318 a          360 c          276 g          282 t
ORIGIN      
        1 tcccattgga ctgtattcca tgactttaag gttttcttca gtataccccg agtggcaatc
       61 atctcgccat cgccaaatac gacatcaaag gaaacccgaa accagtcgct tttgaacgta
      121 gctatgttca tcaacttcgg aactgcatct ggagggaacc ctccatttct cgtgagatca
      181 acgatcgatt ccaaaggttt ctccgtcctg cttttaacta tttcagcggc caatttctcg
      241 tcgagccctt ccaaaagtga aagaacctct actggcgcta cgttcatgtt gattttccct
      301 gacgaccaaa tagttgccag ggcgcgcaca cccggcttcc cgccaaaccc tgagagagtt
      361 tccggtttca tatcgggtac taaaagcagc tcctccaagg aaagaagctt cctgttcaga
      421 ttgccggtgc gctccccgcc tcccaaacga ggttcagtat cctcgtccag aaaatcaagc
      481 acacggttct ccaactgctc agctccaact ttttgccaca gcgccgacca tggcgcctcc
      541 atctctcttc gcagagtctt cccgtccggc agaaaaagat tattcagagg aatgcgatcg
      601 tcaagcggct ccatcctcat cgtaaccgaa acgccggcat ccggaaaatc aaaagtacgg
      661 ggagcgaaaa attcgtcctc ggcagaatgt gatttcccat tgtgcttgga aaggagaagt
      721 ttcgccgcat ccattgcaac ttccgaaacc atgcgggcgg taaaaattcg ttgttccctg
      781 tcaaaacgcc tcattgtacg gcgggcaaag atcgcgaatc cggtagacgc ggagacaaga
      841 aaaacagaaa tcagcagcac ggagagaagc accacgccgc tccttttaga actcgatatt
      901 ctattgagga ggaagccagt cttcatgaac aaactcctcc tcacctattt ttactgtgat
      961 gcggataccg cgaggacggt tccctttata ttcgtcgctc cattcattct ggtcgaaagg
     1021 aagaagcgca aaacgaaggg attccgcctc cgaaaaaagg agcttccctt tttcaggctc
     1081 gagcatgtcc caatttactg atccgggagt gaggaacggg agaatccata aatacactcc
     1141 gggttcccct atctttcccg ccgagggaag aaaacgccgc accgcgcgca cacccggttc
     1201 ccccccgcgg tcgctccaga aagcaagcct gtcgtc
//
LOCUS       DAOPKU010000756         1233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_135488_length_1233_cov_4.802207, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000756 DAOPKU010000000
VERSION     DAOPKU010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135488_length_1233_cov_4.802207"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..307)
                     /locus_tag="PK849_15560"
     CDS             complement(104..307)
                     /locus_tag="PK849_15560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPE67600.1"
                     /translation="MAQRIVDYMDRRVAMMDNPRTLGKALKGGFGDFWRYRVGDYRVI
                     CSIEDKNVMVLVVRVGHRSDVYR"
     gene            complement(354..587)
                     /locus_tag="PK849_15565"
     CDS             complement(354..587)
                     /locus_tag="PK849_15565"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HPE67601.1"
                     /translation="MPTPISVRLDEETGKRLDRLAKETGRTKAFYIRKMIEERIDDME
                     DYYLAAERLAQSRKFGSRSLTSEEARRELGLED"
     gene            complement(761..>1233)
                     /gene="trpA"
                     /locus_tag="PK849_15570"
     CDS             complement(761..>1233)
                     /gene="trpA"
                     /locus_tag="PK849_15570"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPE67602.1"
                     /translation="FAKDAADAGVAGVIVPDLPFHQSEELAGLLRSRGVDFILMVTPN
                     STPERLKAIAEKASGFLYCVSLLGVTGADEKGNAHLEEYMQRVRASTKLPLALGFGVS
                     TPQRAKAAAQVADGVIVGSALISALEPCGTDRKQLAAKATEFIGSLRAGVDSAG"
BASE COUNT          245 a          390 c          327 g          271 t
ORIGIN      
        1 aggaacagag agaaaacggt agaatacaag caacgcctca tagcataccc ctcccaaaaa
       61 aactcgttgc atcgtcccga aatacatcca tatatgcttc accttatctg tacacatcac
      121 tgcggtgacc tacacggact accaaaacca tcacgttttt gtcctcaatc gaacaaatta
      181 cacggtaatc tcctacacga tagcgccaga aatcgccaaa tcctcccttg agcgctttcc
      241 ccaaagtcct gggattgtcc atcatggcga ctcttctgtc catataatcg acgatacgct
      301 gcgccatccg acggtcgata tgccgaaggt ctccaagtgc cgaaggatcg tattcaatcc
      361 tccaggccaa gttcacgcct cgcttcttca gatgtcaggc tgcgggagcc gaatttccgg
      421 ctttgcgcaa gtcgttccgc ggcaagataa tagtcttcca tgtcgtcgat ccgctcttct
      481 atcatcttcc gtatgtaaaa cgctttcgtc cgcccggtct ctttcgccag ccggtccagc
      541 cgttttccgg tttcctcgtc cagtcgcacg gatattggcg taggcatagc aacgcctcct
      601 tttttaaaac gtagtctttg tagtcattgt agcgcgccga tcgatgctgt caaccaaaaa
      661 acgcgccgcg acatgcctga tcggcacaat cgcaagcgcg cgtttcactc tgtattccgt
      721 tgtccgttgc cgaaagcggc cgctttcgcc gaacttccct ctaccccgcc gagtcgacgc
      781 ctgcgcggag ggagccgatg aactccgtcg ccttggccgc gagctgtttt ctatcggttc
      841 cgcacggctc cagcgcggag atgagcgcgc tgccgacgat cacgccgtcg gcgacctgcg
      901 ccgctgcctt cgcccgttgg ggcgtgctca cgccgaagcc gagggcgagc gggagcttcg
      961 tggaggcgcg gactcgctgc atgtactctt cgaggtgcgc gtttcctttc tcatcggcgc
     1021 ccgtcacccc gaggagcgag acgcagtaga ggaagccgga cgccttctcc gcgatggcct
     1081 tcaaccgttc gggggtgctg ttgggcgtga ccatgaggat gaagtccact ccgcgggaac
     1141 gaagcagccc cgcgagttct tcgctctgat ggaaggggag gtcggggacg atgacgcccg
     1201 cgacgcccgc gtcggcggcg tccttcgcga agg
//
LOCUS       DAOPKU010000757         1228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_136333_length_1228_cov_3.465473, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000757 DAOPKU010000000
VERSION     DAOPKU010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136333_length_1228_cov_3.465473"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..>1228
                     /gene="gcvH"
                     /locus_tag="PK849_15575"
     CDS             413..>1228
                     /gene="gcvH"
                     /locus_tag="PK849_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPE67603.1"
                     /translation="MGYDFQSIEKKWQHTWESEGTFNADADPARKKFYCLEMFPYPSG
                     ALHMGHLRNYSIGDMMTRFLWKRGFNVLHPIGFDAFGLPAENAAMKYKTPANKWTWEN
                     IEHMTEQLKHMGCSYDWRRRVETCNPDYYKWTQWIFLQFFKKGLAYRKNAPVNWCESC
                     NTVLANEQVINDGHCWRCNTPVVKRNLEQWFLKITDYAQEQLGDLTFVELPQPGDMAA
                     AGEEIGSVESVKAASEIYSPVSGEVIEVNTTLEDSPELINQDAFGDGWMFKVRM"
BASE COUNT          302 a          344 c          362 g          220 t
ORIGIN      
        1 tcacccatca cgctccagct ccgctacctg caaacgctgc gcgaagtcag cggcggagac
       61 aaatcctcca caaccatctt cccgctgccg ctcgacctgg tgaagccctt tatggagatg
      121 ttcttcgaca aggataagaa aaaagcgggc ggcggcgacg cgtaagcgtc aatagtcggc
      181 aatgggaggc ggcaggctgc cgcacgcggc ctgccccgcc cgtaaacaaa acggatagtg
      241 gaatcaggcg attccggtat ataatacctg tacaagacca tttcggacga ctgttttgaa
      301 acggacgcac acggcctttc aaggagagag ccttcacgag agaagacttt cttccgggac
      361 ggtcgtttgc gtctgtcctg cgaacgggcg ctgtgatcaa ggaggaaggg ttatgggcta
      421 cgattttcaa tccatcgaga aaaaatggca gcatacgtgg gagagcgaag ggacgttcaa
      481 tgcggacgcc gatccggcac ggaagaaatt ctactgcctg gagatgtttc cgtaccccag
      541 cggcgcgttg cacatggggc acctgcggaa ctactccata ggcgacatga tgacccgttt
      601 cctgtggaaa cgggggttca acgtcctgca tccgatcgga ttcgacgcgt tcggtctccc
      661 ggccgagaac gccgcgatga agtacaagac tcccgccaac aagtggacct gggagaacat
      721 cgaacacatg acggaacagc tcaaacacat ggggtgcagc tacgactggc ggcgaagggt
      781 cgagacctgc aaccccgact actacaagtg gacccagtgg atcttcctcc agttcttcaa
      841 gaaggggctc gcctacagaa agaacgcgcc ggtgaactgg tgcgagagct gcaacacggt
      901 gctcgccaac gaacaggtca tcaacgacgg gcattgctgg cgatgcaaca cgcccgtcgt
      961 caagcgcaac ctagagcagt ggttcctcaa aattaccgac tatgcgcagg agcagctcgg
     1021 cgatctgacc tttgtggaac tgccccagcc cggcgatatg gccgctgccg gtgaggaaat
     1081 cggcagtgtg gaatccgtca aggcggccag cgaaatctat tcgcccgtga gcggtgaagt
     1141 catcgaagtg aacaccacgc tggaagactc gccggaactg atcaatcagg atgcctttgg
     1201 cgacggctgg atgttcaagg tccgcatg
//
LOCUS       DAOPKU010000758         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_137341_length_1222_cov_3.517566, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000758 DAOPKU010000000
VERSION     DAOPKU010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137341_length_1222_cov_3.517566"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PK849_15580"
     CDS             complement(<1..527)
                     /locus_tag="PK849_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67604.1"
                     /translation="MDSRRSRRIFLFCAALLSFALLFLVARGVFVRFFLPEAASVSVT
                     VLEASRFEPVEILLAQPTLVALIAWLSPFRSFGALWPSVCAGAGLLLAIALLYPLRAS
                     SHPFFALLFFVPAVLSAPFLFMLTADPASAIAVACLSVAVADMLSHLRTGRTFPLFSA
                     GLALGLVPFAASWGL"
     gene            complement(508..>1222)
                     /locus_tag="PK849_15585"
     CDS             complement(508..>1222)
                     /locus_tag="PK849_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPE67605.1"
                     /translation="LTKLFTLEYSFWFDYMLPGIEALGMPTPLGGTSNHFRTEMLRLL
                     GGWDPFNVTEDADLGIRASAHRMWVSTINSTTFEEANSHTGNWIRQRSRWIKGYMQTY
                     LVHMRNPLRLFRTIGLMPFLGVQLLIGGTFMTFLINPILWLLFIFWIAFRPLWMESVF
                     PLWILTLSTLNLIVGNAMGIYLNMLAVFRRRLFHLTFYSLMNPFYWILHSIAAYKALW
                     QLFTKPFFWEKTVHGLAKK"
BASE COUNT          333 a          330 c          383 g          176 t
ORIGIN      
        1 tacagccccc atgaagcggc gaagggaacg agaccgagcg ccagcccggc gctgaagagc
       61 ggaaacgtgc gccccgtacg caagtgagag agcatatcgg cgacggctac cgagagacag
      121 gcgaccgcaa tcgcagacgc gggatctgcc gtcagcatga agaggaacgg cgccgaaaga
      181 accgccggaa cgaagaagag caaagcaaag aaagggtgcg aagacgcgcg cagaggataa
      241 aggagggcga ttgcgagcag gaggccagcc cccgcgcata cggagggcca aagagccccg
      301 aacgagcgga acggcgagag ccatgcgatc agcgctacaa gagtcggctg cgccagcaaa
      361 atctcgaccg gttcaaatcg ggacgcttcc agcaccgtga cggagactga ggcggcttca
      421 ggcaggaaga aacgcacgaa gacaccccgc gcaacgagaa agagcagcgc gaaggagaga
      481 agcgcggcgc agaatagaaa aatccgtcta cttcttcgcg agtccatgga ctgtcttctc
      541 ccagaagaac ggttttgtaa aaagctgcca gagcgccttg tacgcggcaa tcgagtgcaa
      601 aatccagtag aacggattca tgagcgagta gaatgtcaga tggaagagtc tgcggcggaa
      661 gaccgcgagc atgttcagat aaatccccat ggcgttgccg acgatcaggt tcagcgtgga
      721 gagggtcaaa atccagagcg ggaagaccga ctccatccac aggggacgga aggctatcca
      781 gaaaataaaa agcagccaga ggatcggatt gatgaggaac gtcatgaagg ttcctccgat
      841 caggagctga acgcccagaa atggcatgag accgatcgtc cggaagagcc gtagcggatt
      901 gcgcatatgc accagatacg tctgcatgta ccccttgatc caccgggaac gctgtcggat
      961 ccagttgccg gtatggctgt tcgcttcttc gaaggtggtc gaattgatgg tggagaccca
     1021 catcctgtgg gcgctcgccc gtattccaag atcggcgtcc tccgtcacgt tgaaggggtc
     1081 ccaccctcca agcaggcgga gcatctccgt ccggaagtga ttcgacgtgc cgccgagcgg
     1141 agtgggcatc cccagcgcct cgattccggg cagcatatag tcgaaccaga acgaatactc
     1201 caacgtgaat aacttcgtca gc
//
LOCUS       DAOPKU010000759         1219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_137868_length_1219_cov_3.123711, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000759 DAOPKU010000000
VERSION     DAOPKU010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137868_length_1219_cov_3.123711"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PK849_15590"
     CDS             <1..534
                     /locus_tag="PK849_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67606.1"
                     /translation="LGLVVAKYADELLGGLLTPAVLAAGPVPNGRCEDFITDCVQPYE
                     WWTGFNGGGAGAPALSSVKDCLRVPSTLGDALNLYSYTFKGKTLDVDESMSFLKFREH
                     ISPKCAEHLRRRTVKRIERIVKSDPGCKEVQDELCEVFSRAAEQGWDTEELGRKIVAV
                     RAAADEVFSDPSENPVL"
     gene            755..>1219
                     /locus_tag="PK849_15595"
     CDS             755..>1219
                     /locus_tag="PK849_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67607.1"
                     /translation="MKKKGANSMVADKQTGCSTRRDAENDNVLFSSIETAMLAALDSY
                     LWKNDRDEFLGNLEMLSGALSLVKPERLRPLLRLSSTFVNTCLHMVRIWNCGSGLRVE
                     NGMFSSPPSVPFIAFLLAPTGFPFTEEEMDSVRGASGYGVEDVRKWHEKVMSR"
BASE COUNT          236 a          290 c          372 g          321 t
ORIGIN      
        1 cttgggcttg tggttgcgaa gtatgccgat gaactgctcg gcggtctgct gactccggca
       61 gttctggcgg ccggtcctgt gccgaacggg cgctgcgagg atttcattac tgattgcgtc
      121 caaccctacg agtggtggac cggattcaac ggcggcggag cgggtgcgcc cgctctttcc
      181 tcggtgaagg actgcctgcg cgttccatct actctaggcg atgcgctgaa tctgtattcg
      241 tacacgttca agggaaagac gctggacgtc gacgagagca tgtcgttttt gaaattccga
      301 gagcacatat cgcccaagtg cgccgagcat cttcgtcggc gtacggttaa gcggatagag
      361 cgcattgtga agtctgatcc gggctgtaag gaagtacagg atgagctttg cgaagtcttt
      421 tcccgggctg cggaacaagg atgggacacg gaggagctgg ggagaaagat tgttgccgtc
      481 cgtgccgcag cagacgaagt gttttcggat ccttcggaga atccggtgct gtagcgccgc
      541 ttcttttgat tcatattgca tagtgccccg gtaacggcag ggtggtttcc gcctcattca
      601 cgtcctgccg ttacgttttt cctcccttct gtttctctga atatgctcgc ttgacgggta
      661 gatatagata tggtatatta tgttcatccc ttcttctccg gattttcggc gtttcggagg
      721 ggtaagatgg tcttttattc ggtgagaagt ctccatgaaa aagaaagggg cgaacagcat
      781 ggtcgcggac aagcaaacag gttgcagtac ccggcgggat gcggaaaacg ataatgtttt
      841 attctcttcg atcgaaacgg cgatgctggc cgctctggat tcttacttat ggaagaacga
      901 ccgcgatgag tttctcggta atctggagat gctctccggc gcgctttccc tcgtgaaacc
      961 cgaaaggctt cgccctcttc tgaggctgag ttcgactttt gtgaacacgt gccttcacat
     1021 ggtccggatc tggaattgcg ggagcggatt gcgggtggaa aacgggatgt tttcttcccc
     1081 tccgtccgtt ccttttatcg catttctgct tgcgcctacg ggatttccgt ttacggagga
     1141 agagatggat tccgtacggg gcgcttcggg gtacggggtt gaggatgtcc gcaaatggca
     1201 cgaaaaggtg atgagtcgt
//
LOCUS       DAOPKU010000760         1218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_138001_length_1218_cov_4.709372, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000760 DAOPKU010000000
VERSION     DAOPKU010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138001_length_1218_cov_4.709372"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PK849_15600"
     CDS             complement(<1..399)
                     /locus_tag="PK849_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPE67608.1"
                     /translation="MSGAFFGSTDALRERYNTITPEILAELKAAVGAKNVVAGDPDAL
                     ESYASDEAGIMFTSMPDAVVKAENTEHVSAVMQLASKYKIPVTPRGAGSGLAGAAVPL
                     CGGIVLSMEKMNRILEIDPINRVAVVEPGVV"
     gene            complement(396..>1218)
                     /locus_tag="PK849_15605"
     CDS             complement(396..>1218)
                     /locus_tag="PK849_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPE67609.1"
                     /translation="IAGGADQVYVVDSPELASFLPDSYTKTLAALVERHKPEIFIASA
                     TSTGRTIMPLLYVDAETGLTADCTELTIEKAERLLLQTRPAIGGNVMADIRTPLGRPQ
                     MCTVRPRSKKPLPVDRSRTGKVFRESVDKRDLSSVMKLLEFLPEKNVGAPLQDAEIIV
                     SGGKGMKNAKNFAMLEELARLLGGSVGASRTAVDLNWAPYSSQVGLSGKSVTPKLYIA
                     CGISGAVQHLAGMSASDTVIAINTDPEAPIFRVADLAIVGDVLEVVPALIGRLKS"
BASE COUNT          186 a          400 c          405 g          227 t
ORIGIN      
        1 gaccacgccc ggctccacca ctgcgacgcg gttgatcggg tcgatttcga ggatgcggtt
       61 catcttctcc atcgagagga cgatgccgcc gcacagcggg accgccgccc cggcgagtcc
      121 gctccccgcg cccctcggcg tgaccggaat tttgtacttg gatgcaagct gcatcacggc
      181 ggagacgtgt tccgtgttct ccgccttcac aacggcgtcc ggcatggagg tgaacatgat
      241 gcccgcctcg tcggatgcgt agctttccag agcgtcgggg tcgccggcga cgacgttttt
      301 cgcgcccacc gccgccttca gctccgcaag tatctccgga gtgatcgtgt tgtagcgctc
      361 gcgcagagcg tccgtgctgc cgaaaaatgc gccgctcatg atttcagcct ccctataagg
      421 gcggggacga cttcgaggac gtcccccacg atggcgaggt ccgccacgcg gaagatgggg
      481 gcctcggggt cggtgttgat ggcgatgacc gtgtccgacg ccgacatccc cgcgaggtgc
      541 tgtaccgcgc cggagatgcc gcaggcgatg tagagcttcg gcgtgacgga ctttccggag
      601 agtcccacct gcgaggagta gggggcccag ttcaggtcga ccgcggtgcg cgacgcgccg
      661 acggagccgc cgaggagcct cgccagctct tcgagcatcg cgaagttctt cgcgttcttc
      721 atccccttgc cgccggagac gatgatctcc gcgtcctgca gcggcgcgcc gacgttcttc
      781 tccggaagga attcgagcag cttcatcacg gaggagaggt cccttttgtc cacgctctcg
      841 cggaagacct ttccggtccg cgaacggtcc acgggaagcg gtttcttcga gcgggggcgg
      901 acggtgcaca tctgcggccg tccgagcggg gttctgatgt ccgccatgac gttgcccccg
      961 atagccgggc gcgtctgaag cagcaggcgc tccgccttct cgatggtcag ctccgtgcag
     1021 tcggcggtga gccccgtctc cgcgtccacg tagagcaggg gcatgatggt gcgccccgtc
     1081 gacgtggcgg aggcgatgaa gatctccggc ttatgccgtt ccaccaacgc ggcgagcgtt
     1141 ttcgtgtagc tgtccggcag gaacgacgcc agttcgggcg agtccaccac gtagacctgg
     1201 tccgcgccgc ctgcgatc
//
LOCUS       DAOPKU010000761         1218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_138049_length_1218_cov_2.804815, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000761 DAOPKU010000000
VERSION     DAOPKU010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138049_length_1218_cov_2.804815"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK849_15610"
     CDS             <1..240
                     /locus_tag="PK849_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67610.1"
                     /translation="HTKVVPIVSIGSGTHIGAAQIAGPRGEPVGMTKAVAQIEATFRD
                     RLRVKILVPVDSENPVQRFRRVQGVGVSAIIDFKL"
     gene            274..>1218
                     /locus_tag="PK849_15615"
     CDS             274..>1218
                     /locus_tag="PK849_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67611.1"
                     /translation="MKRLFFVIVFLCCLSAPSFGSGSIVEATSILERWTAFHWGRDCL
                     VWLVHYPEELIDPWVAAEAARSGMSEAESAQYRASFTSELRIAETEPFLLTVYVFGPR
                     PLDLGSFASAVQLQTNEGKKVPPLSYERKFDQPLSGIVQGLVFFPKQPEKGFSVIIRN
                     LGIQEEQHFVFDRADGPLVAAAEPEVVVVELPPAPKKEPVKPQPPKREKPTPTPPPPP
                     PVATPQELAELKPEPVVVDLPAPEKEKPVLYISKEKTVENFIRHWIEGQTSAMYDLLS
                     SSTRTALTEEQFARQVDATGLRNSLREGYKIQWMDDSRV"
BASE COUNT          236 a          320 c          372 g          290 t
ORIGIN      
        1 catacaaagg ttgttcctat tgtttccatt ggttccggaa cgcacatagg cgcggcgcag
       61 attgccgggc cgagaggaga acctgttggg atgacaaagg cagtggctca gatagaggca
      121 accttccggg atcgtctgcg cgtgaagata ctggtgccgg tggattctga gaatccggtg
      181 cagcgcttcc ggcgggttca aggagtcggt gtttctgcga tcattgattt taaactgtag
      241 ggtgatcaat cttttgtttt tggggtggcg ttcatgaaac gactgttttt tgttattgtg
      301 tttctttgct gcctttctgc cccgtctttc gggagcggtt ccatcgttga ggcgacgtcc
      361 attcttgaac gatggacggc ctttcactgg gggcgcgact gtcttgtatg gcttgtgcac
      421 tatccggagg agcttattga cccctgggtt gctgccgagg ccgcgcgcag cggaatgagc
      481 gaggcggaga gcgctcagta ccgcgcttcc ttcacttccg agctgcggat agcagagacg
      541 gaaccttttc tgctgactgt gtacgtcttc ggccctcgcc cgcttgacct gggctcgttt
      601 gcgtccgctg tccagctgca gacgaacgag gggaagaaag ttcctccgct gagttatgaa
      661 cgtaagttcg atcagccgct ctccggtatc gtgcaaggcc ttgtcttttt cccgaaacag
      721 ccggagaagg gcttcagtgt gattatccga aaccttggca tccaggaaga gcagcatttt
      781 gtttttgatc gtgcggacgg ccccttggtt gccgcggcgg aaccggaagt agtggtggtc
      841 gaactgccgc cggcgccgaa gaaggaaccc gtgaagcctc agcctccgaa gcgcgagaaa
      901 ccgactccca ctccgcctcc tccccctccg gtggcgacgc ctcaagaact tgcggagctg
      961 aaaccggaac ctgtggttgt cgatctgcct gctccggaga aggagaagcc cgttctctac
     1021 atcagcaagg agaagactgt ggagaacttt atccgccatt ggatagaagg gcagacatcg
     1081 gcgatgtacg accttctttc ctcctccacg aggactgcgc tcacggaaga gcagttcgcc
     1141 aggcaggtgg atgccacggg cttgcgaaac tcccttcggg aggggtataa gattcaatgg
     1201 atggacgata gccgcgtc
//
LOCUS       DAOPKU010000762         1216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_138420_length_1216_cov_2.382429, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000762 DAOPKU010000000
VERSION     DAOPKU010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138420_length_1216_cov_2.382429"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..>1216)
                     /gene="dgt"
                     /locus_tag="PK849_15620"
     CDS             complement(224..>1216)
                     /gene="dgt"
                     /locus_tag="PK849_15620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HPE67612.1"
                     /translation="IGAAFEAAIDRGEIALDAFMRDKGGFEGNGQTLRILSKLGKYSE
                     SHGMDLTRRSLLGVLKYPANYSAVKNGKVFEEDGNNPPPKCYHDDETGVVEWILESFP
                     PEDRKRFIETTERVDTPEAKHGKPLHKGLDTSIMEIADDIAYGVHDMEDAIALNMLKE
                     RDCERLKQIVPDDLQQGGELITALFSNKKYEAKHAIGALVNYFITRVTLRRKEAFEHP
                     LMQLNAVLSEDAASFLDEFRKLIKERVIFSQNVQLLEQKGKLMIREIFTKLKEAPDRY
                     LPEDTRRKCVVPQGNGEENYRIICDYVAGMTDEYACKIYQMMFLPGYGSVFNRL"
BASE COUNT          222 a          330 c          347 g          317 t
ORIGIN      
        1 gcatactatt cacctcagct ttcgcggtat gtccgccact ggaaagagct gacgaaacag
       61 gaaacctgcg gactcctctg gttcgtcgaa accggagaat tcttcgatga gaaggggatg
      121 cgggtggaag ggtaggggaa gagcggattt ttatagaaag aaaacggcga gatgtttggt
      181 ggagcggtac tgcttgctca tgcacatcag cttcgccctt ggcttacaac ctgttgaaca
      241 ccgagccgta cccggggaga aacatcatct ggtagatttt gcaggcgtat tcgtcggtca
      301 tgcctgcaac gtagtcgcag atgatcctgt agttttcttc gccatttccc tgcggtacaa
      361 cgcactttcg gcgggtgtcc tccggaaggt agcggtcggg ggcttccttt agcttggtga
      421 atatttccct gatcatcagc ttgcccttct gctcgagcag ttggacgttc tggctgaaaa
      481 tgacgcgctc tttgatgagc ttcctgaatt catccaaaaa ggaagccgcg tcttctgaga
      541 ggacggcgtt gagctgcatc agggggtgct cgaaagcctc tttccggcgc agcgtgacgc
      601 gagtgatgaa gtagttcacc agcgccccga ttgcgtgttt cgcctcgtat tttttgttcg
      661 aaaacagggc ggtaatcagc tctccgccct gttgaagatc atcggggact atctgcttca
      721 agcgctcgca gtcgcgctcc ttcagcatgt tcagcgctat ggcgtcctcc atgtcgtgga
      781 cgccgtaggc tatgtcgtcg gcgatctcca tgatggaggt gtccagcccc ttgtgcagag
      841 gctttccgtg tttcgcctcc ggtgtatcca ccctttcagt tgtttcgata aaacgtttcc
      901 ggtcttccgg cggaaatgac tccagtatcc actccaccac gccggtttcg tcgtcgtggt
      961 agcacttcgg tggaggattg ttcccgtcct cttcgaagac tttcccgttt tttaccgcgc
     1021 tgtagttcgc cgggtacttg agcaccccga ggaggcttct gcgcgtcagg tccattccgt
     1081 gggattccga gtattttccc agttttgaga ggatgcggag cgtctgtccg ttcccttcga
     1141 aaccgccctt gtcgcgcatg aaggcgtcga gggcgatctc gccgcgatcg atcgccgcct
     1201 cgaaggcggc cccgat
//
LOCUS       DAOPKU010000763         1209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_139613_length_1209_cov_2.985269, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000763 DAOPKU010000000
VERSION     DAOPKU010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139613_length_1209_cov_2.985269"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1209)
                     /locus_tag="PK849_15625"
     CDS             complement(<1..>1209)
                     /locus_tag="PK849_15625"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPE67613.1"
                     /translation="FLDIDDPQIEAALTDTGRLEAMCPEPLIQFNPSYETGARVEDLA
                     AEGVLAPGMSSVFGNFVLHRHQEEALRLGSAGKSFVVTSGTGSGKSLTFLGTIFNEVL
                     RNSSPGVAGLVVYPMNALINSQTGEILKYADLYKKNTGKDFPVTHGQFTGQEDEEKRE
                     RIRSDPPHILLTNYMMLELILTRGGDERIKNAIFSNLKYLVFDELHTFRGRQGADVAL
                     LIRRIKAECANDVVCIGTSATMAGGANAAERKSRVAEVASTFFGTPFSSEQVIEESLR
                     AISPGGEALRREDLLHALENPYAPSGEDDAESFAASPLFRWLERNVALAPDNGNFQRG
                     EPKTLSAIVALLSADSGAGPEECDRALRTLFDTIASVNKKIAERNELSATGRPFLLPF
                     KLHQFIAQSAT"
BASE COUNT          206 a          346 c          397 g          260 t
ORIGIN      
        1 ggtggcggac tgcgcgatga actgatgcag cttgaagggg aggaggaacg gcctcccggt
       61 ggcggacaat tcgtttctct cggctatttt cttgttgacg gaggcgatgg tgtcgaagag
      121 cgttctgagg gcgcggtcgc actcttccgg accggccccg gaatccgcgc tcaacagcgc
      181 gacgatggcg gagagcgttt tcggctctcc tcgctggaag ttcccgttgt ccggcgcgag
      241 cgcgacgttc cgctcgagcc agcggaagag cggagaagcg gcgaacgact cggcgtcgtc
      301 ttctccggag ggcgcgtacg ggttttcgag cgcgtggagc agatcctcgc ggcgaagagc
      361 ttctcctccg ggcgatatcg cccgcagaga ttcctcgatc acctgttcgg aggaaaaggg
      421 cgttccgaaa aaagtcgagg cgacttcggc gaccctgctc tttcgttcgg cggcgtttgc
      481 gcctccggcc attgtcgccg aggttccgat gcaaacgaca tcgttggcgc attcggcttt
      541 gattctgcgg ataaggagcg cgacgtccgc cccctgtctg cccctgaacg tatggagttc
      601 gtcgaagacg aggtacttga ggttggagaa gatcgcgttc ttgatacgct cgtcgccgcc
      661 gcgggtgagt atcagctcga gcatcatgta gttcgtcagc aggatgtgcg gcgggtcgga
      721 gcggatccgt tcgcgcttct cctcgtcctc ctgccccgtg aactgcccgt gcgtgacggg
      781 gaagtctttt cccgtgttct ttttgtacag gtcggcgtac ttgagtatct ctccggtctg
      841 ggagttgatg agggcgttca tgggatagac gacgagcccc gcgactccgg gagaggaatt
      901 ccggagcact tcgttgaaga tcgttcccag gaaggtcaga gatttgcccg aaccggttcc
      961 cgaggtgacg acgaagctct tcccggcgct tccaaggcgc agcgcttcct cctgatgcct
     1021 gtgcaggacg aaattcccga aaacggagga catgccgggg gcgagcacgc cttcggccgc
     1081 gaggtcttcg accctcgccc ccgtctcgta ggacggattg aactgaatga gcggctccgg
     1141 acacatggct tccagccttc cagtgtcggt caaggccgct tctatctgcg gatcgtctat
     1201 atcgaggaa
//
LOCUS       DAOPKU010000764         1205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_140367_length_1205_cov_2.567826, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000764 DAOPKU010000000
VERSION     DAOPKU010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140367_length_1205_cov_2.567826"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1205
                     /locus_tag="PK849_15630"
     CDS             <1..>1205
                     /locus_tag="PK849_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE67614.1"
                     /translation="RAKNLYSVQDYRGALALFDRLLGEIPGDGEALDYSAWCLRYLGD
                     WKSAEERFFQALDAPSGALTSWIHVGLGETYLGAGDERKAVASFQKAIESAPGDDELF
                     LRSQKGIAWAAAFLGEREKHGRALALIKEKDGKFAEDVLGDTGAILEEREALAAAKKT
                     FDEPVEPILVLDTMERHAKLYEEEALEDELDEILSEVLVQETPAAEKQEDVLEIIVLN
                     DVKTGGDVASSGEKEETPGPDETPPGDTTITPLPTPGAALEKPEESAGPVLSKQKPAK
                     KPSQKKPEEKKTAQSGATVSPPSAVWGVRIGVALDEELTRAQGEGLAVEKESTTDKYG
                     VRRFPFTPKITPFPSSVRSQAASEFYHIEGYKGTILKVHGMVKTKLLPKPLEWKKATF
                     DAVVDEYSK"
BASE COUNT          297 a          311 c          376 g          221 t
ORIGIN      
        1 acgggcgaaa aatctgtata gcgtgcagga ctaccgggga gccctcgcgc tcttcgaccg
       61 gttgcttgga gagatcccgg gcgacggcga ggcgcttgac tattcagcgt ggtgcctgcg
      121 ttacctcggc gactggaaga gtgcggaaga gcgtttcttc caggctcttg acgctccgtc
      181 aggagcgctg acgagctgga ttcacgtggg cctcggcgaa acatacctcg gcgcgggcga
      241 tgaacgcaaa gcggttgcat ccttccagaa ggcgatagag agcgctccgg gcgacgatga
      301 gctttttctt cgttcgcaga aggggattgc ctgggccgcg gcatttctcg gagaacggga
      361 gaaacacggg cgggcgctcg ctctgatcaa ggaaaaggac gggaagttcg cggaggacgt
      421 tctgggcgac acgggagcaa tcctagagga acgcgaggcg cttgcggccg caaaaaaaac
      481 ttttgatgaa ccggtcgagc cgattcttgt gctggacaca atggaacggc atgcgaaact
      541 ttacgaagag gaagctctgg aggatgagct ggacgagatt ttgtcggagg tcctcgtgca
      601 agagacgccg gctgcggaaa aacaagagga cgtccttgag attattgtgc tcaacgacgt
      661 aaagactggc ggcgacgtcg cttcttccgg agaaaaagag gagacgcccg gaccggacga
      721 aacgcctcct ggcgacacga cgatcacgcc gcttcctact ccgggtgcgg ctttggaaaa
      781 gccggaggaa tctgcgggac ctgtgctctc gaagcaaaaa cccgcaaaaa aaccttcaca
      841 gaagaagcct gaagagaaaa aaaccgccca gagcggcgcc actgtctctc ctccttctgc
      901 tgtatggggc gtccgtatcg gagttgctct tgacgaagaa ctcacaaggg cgcagggcga
      961 aggcctcgcg gttgaaaagg agagtacgac cgacaagtac ggtgtacgcc gtttcccctt
     1021 tacaccgaag attactccct tcccgtcatc cgttcgttcg caagcggcat ccgagttcta
     1081 ccacatagaa ggctacaagg ggaccatcct gaaagtacac ggtatggtga agacaaaact
     1141 cctgccgaag cctcttgaat ggaaaaaggc gacatttgat gcagtagtgg acgagtattc
     1201 gaaag
//
LOCUS       DAOPKU010000765         1203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_140711_length_1203_cov_2.700348, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000765 DAOPKU010000000
VERSION     DAOPKU010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140711_length_1203_cov_2.700348"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..>1203
                     /locus_tag="PK849_15635"
     CDS             70..>1203
                     /locus_tag="PK849_15635"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPE67615.1"
                     /translation="MNGSSEKLSFAPFFKAIGGRQDVRAVLDVAAEYLKTGAAFREAY
                     TGNVSVVSSSEIFRESAKTFPMLELSRIYCSREVQVGNVHSGILIFDIPSDRAHALSD
                     EESIYIENAVIATALCLERSFSRTLPGNEKRDELLKDLLAGEVRDYREAEESFAQWGW
                     KKDQEFLVLVLRLADDVKEDDGFSARERHISFSLARSKVLPFFPKSFCVFYTKMPVFL
                     LPLPSGKPEVLSGVKDVLESILLSLKDELPPKKGRAFLFAAGGAKRGVNAIHRSFDEA
                     VRVLSVSESLRREGGVILWDMLGGYQLVAILSETEEARLFCGRILGELLEGTDSSSVE
                     LLETLLVMDGCNWNVRAASESLRFHYNTVKYRLGVLRERIGFDS"
BASE COUNT          244 a          295 c          370 g          294 t
ORIGIN      
        1 aaggccgggg ggctttcggt ttcaggagag gcgtttgaaa aacccgtgca tgcggggagg
       61 tgacattgaa tgaacggttc cagtgaaaaa ctgtcttttg ctccattctt caaagcgatt
      121 gggggcaggc aagatgtccg ggccgttctc gatgttgccg ccgagtatct gaagacggga
      181 gcagccttcc gggaggcgta cacgggaaac gtcagcgtcg tctcctcttc cgaaatcttt
      241 cgggaaagtg cgaagacgtt tccgatgctt gagctgtcca gaatatactg ttcccgcgag
      301 gtgcaggtcg ggaatgtcca ctccggtatc ctcatcttcg acattccgtc ggaccgcgcc
      361 cacgctcttt cagacgagga atcgatctat atcgagaacg ccgtgatcgc gacggcgctc
      421 tgcctggagc gttctttcag ccgcacgctt cccgggaatg agaaaaggga cgaactcttg
      481 aaagatttgc tcgccgggga ggtgcgcgac tacagggaag cggaggagtc gtttgcgcaa
      541 tgggggtgga aaaaggacca ggagtttctt gtgctggttc ttcgtcttgc ggacgatgta
      601 aaggaagatg atgggttttc ggcgagggag cgtcatattt ccttctcact tgcacgaagc
      661 aaggtgctcc cttttttccc caagtctttt tgtgtttttt atacaaagat gccggtgttt
      721 ctccttccgc tcccctccgg aaaaccggag gtcctttccg gagtgaaaga tgttcttgag
      781 agcatactcc tttcgctgaa agacgagctt ccgccgaaga aaggacgcgc ttttctcttt
      841 gcggccggag gcgccaagag aggtgtgaac gcaattcacc ggagcttcga cgaagcggta
      901 cgagtgcttt cagtctcaga aagcctccgg agagaagggg gcgtcattct ttgggatatg
      961 ctcggcggat accagctggt tgcgatcctc tccgaaaccg aggaggcgcg ccttttttgc
     1021 ggccggatcc tgggcgaact gctggaggga accgattctt cctcagtgga acttctggag
     1081 accttgctgg tgatggacgg gtgcaactgg aacgtcaggg cggcgtccga gtcgctccgt
     1141 tttcattata ataccgtgaa gtaccgcctt ggtgttctgc gggagcggat tggatttgac
     1201 tct
//
LOCUS       DAOPKU010000766         1185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_144040_length_1185_cov_2.368142, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000766 DAOPKU010000000
VERSION     DAOPKU010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144040_length_1185_cov_2.368142"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PK849_15640"
     CDS             <1..816
                     /locus_tag="PK849_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67616.1"
                     /translation="PVFHKGALLKAESLNLLRDVLQKPLWDMLRKGSSGIVSGLSVHS
                     GEEERGVVVAPGVFRIGAHIGWLHDALVLPSPEIRGVYDLGLSFEQEANDSHAPEALW
                     RIEDDDEQKTCVPLRRSTFSLQWIPAPAAVNFERAITLCRIHSTGLSLKNSWIHEREK
                     PRSVKAALLADPADTVLLHYAHHASSGTYPTLCPDMQRFLATLPEFSERRLELLLWFQ
                     TGSFPLCDAARTTDWGEALRWICSLPDEQPERRVVEEPAPRRSESPGIRNIAG"
     gene            829..>1185
                     /locus_tag="PK849_15645"
     CDS             829..>1185
                     /locus_tag="PK849_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67617.1"
                     /translation="MEKTMSPEDIIKKKMVPLQEVANNGKPEEQKGRFTTMLAETFSP
                     KSFFADFNFGTHTPPARDGDWVIGLDRHLVFLFPSIPLGMHYIPFIGRISLSSRSPKA
                     YGKGTVFSKKIAPSGIL"
BASE COUNT          245 a          373 c          329 g          238 t
ORIGIN      
        1 cccgtttttc acaagggcgc gcttttgaag gccgaaagcc tcaatctcct acgggatgtc
       61 ctccagaagc ctctctggga tatgctgcgg aagggaagct ccggcatcgt ctcggggctt
      121 tccgtgcact ccggggagga ggaacggggc gtcgtggtcg cacccggagt gttccgcata
      181 ggagcgcaca tcggatggct ccatgacgcg ctcgtcctgc cttccccgga aatccgcgga
      241 gtgtacgatc tcggtctctc cttcgagcag gaggccaacg actcgcacgc ccccgaagcg
      301 ctctggcgca tcgaagacga cgacgaacaa aaaacatgtg ttcccctccg gcgcagcacc
      361 ttctcgctcc agtggattcc cgccccggcc gccgtgaatt tcgagcgcgc catcaccttg
      421 tgcaggattc actccaccgg actttctctg aagaactcat ggatccacga acgggaaaag
      481 ccccgctccg tcaaagcggc cctgcttgcc gaccctgcgg acactgtgct gcttcactac
      541 gcgcaccatg caagctcggg aacgtacccc acgttgtgcc cggatatgca gcgttttttg
      601 gcgacgctcc ccgagttctc ggaacggaga ctggagctgc tcctctggtt ccagaccggt
      661 tcattcccgc tgtgcgacgc cgcccgtaca accgactggg gggaggcgct ccgctggatt
      721 tgctcgctcc cagatgaaca gccggaacgg cgtgtagtag aggaacccgc tccccgcagg
      781 agcgaatccc ccggaatacg gaacatcgcg gggtaggatg acctcatcat ggaaaagacc
      841 atgtcgcccg aagacatcat caaaaagaag atggttcctc ttcaagaagt cgcgaacaac
      901 ggcaaaccgg aggagcagaa aggcaggttc acaaccatgc tcgccgagac cttttcgccg
      961 aaatcattct tcgcggattt caacttcggg acgcataccc ctccggcacg ggatggcgac
     1021 tgggtgatcg gcttggacag gcatcttgtt tttctttttc cttcgattcc tttggggatg
     1081 cattatatac cgttcatcgg gaggatctcg ctctcgtcgc gttcgcccaa agcctacggc
     1141 aaaggaaccg tcttcagcaa aaaaatcgcc ccgtcgggca tattg
//
LOCUS       DAOPKU010000767         1173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_146257_length_1173_cov_4.098390, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000767 DAOPKU010000000
VERSION     DAOPKU010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146257_length_1173_cov_4.098390"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PK849_15650"
     CDS             complement(<1..786)
                     /locus_tag="PK849_15650"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HPE67618.1"
                     /translation="MTEQSGKLGFDRYLVGEGGKRLFLFLFGGLLCTVGLHSSPFAEI
                     LPGLKLIIFEPDYLVSDYFHVGGRGASFLNSGILTLLFTAILVYYKIHIRGISIAAVF
                     TVAGFAFFGKNLLNVWFILGGVWLYAWYRKESFLKFIYIAFFGTALAPLVSQLMFGLY
                     LPAPFRIAAGAIAGLSAGFILPSLAASFLSVHKGYNLYNVGFTAGMVGTLYVSLFRSH
                     GFVPEARVLWSTGNNGLLLPMLLGMFLTMILAGYLLNGRSFKGL"
     gene            complement(863..>1173)
                     /locus_tag="PK849_15655"
     CDS             complement(863..>1173)
                     /locus_tag="PK849_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67619.1"
                     /translation="ERSMELLSAESGAKYLCFSHYGWTTEVAETLNLAAVQFRDWCER
                     ARRCAAADEEDAVALLFDETQREDPLLGLLSSLRTEQRQKEEFFIKNSLRGIVRWVRS
                     "
BASE COUNT          293 a          346 c          321 g          213 t
ORIGIN      
        1 gagtcccttg aaggatcgtc cgttcaacag atacccggcg aggatcatcg tcagaaacat
       61 cccgagaagc atggggagga gcagcccgtt gttcccggtg gaccagagca ccctcgcctc
      121 cggtacgaaa ccgtgcgagc gaaagagcga gacataaagc gttcccacca tccctgctgt
      181 aaatccgacg ttgtacaggt tgtacccctt gtgcacggaa aggaaggacg ccgccaatga
      241 aggaagaatg aacccggctg aaagccccgc gatcgcgccg gcggctattc gaaaaggcgc
      301 ggggaggtac agaccgaaca tcagctggct cacaagcggc gcgagggctg tgccgaaaaa
      361 agcgatgtag atgaacttca ggaaagactc cttccgatac catgcgtaga gccacacgcc
      421 tccgaggata aaccagacgt tgagcagatt ttttccgaag aaggcaaacc ctgctacggt
      481 gaagaccgcc gcgatggaaa taccgcgtat gtggattttg tagtacacaa ggattgccgt
      541 gaaaagcagc gtgagaattc cgctgttgag aaacgaagct ccgcgtcccc ctacatggaa
      601 gtaatcggag acgagataat cgggttcgaa gataataagc ttcaaccccg gcaggatttc
      661 agcgaacggg ctggagtgca ggcctactgt acaaagaagt cctccgaaaa ggaagagaaa
      721 gagccgtttg cccccctctc cgacgagata gcggtcgaat ccaagcttcc cggactgttc
      781 cgtcactacg atcccccctc ttcccaaagt atgcggcgat accctcctgg gaatgtacgg
      841 ccgcgcttgc cgggccggaa tgttatgatc gtacccatcg gacaatcccc ctcaggctgt
      901 ttttgatgaa gaactcctcc ttttgacgct gctccgtcct caaagaggaa agaagtccga
      961 gcagcgggtc ctcccgttgc gtctcgtcga agagcagcgc caccgcatcc tcttcgtccg
     1021 ccgcagcaca ccgtcgcgcc ctctcacacc agtcgcggaa ctgcacggcg gccagattca
     1081 gtgtttcggc aacctccgtg gtccagccgt agtgactgaa gcagagatac ttcgccccgc
     1141 tctccgccga aagaagttcc atggaacgct cca
//
LOCUS       DAOPKU010000768         1169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_147032_length_1169_cov_3.738779, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000768 DAOPKU010000000
VERSION     DAOPKU010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147032_length_1169_cov_3.738779"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PK849_15660"
     CDS             <1..444
                     /locus_tag="PK849_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE67620.1"
                     /translation="GWSEELSRYLMIWMALLSVALCIWRHEHVGVTMFIKKLPRLLAK
                     IMVFVSNGLILYFLYVLARYGFRMAEGGKSQVSTALHTTMEWWLMAVPVSAVLCIIML
                     VCKMILDIRRKNLDEILMSEDIIDTVKREEGLDFGDVPLKEERSS"
     gene            441..>1169
                     /locus_tag="PK849_15665"
     CDS             441..>1169
                     /locus_tag="PK849_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE67621.1"
                     /translation="MTGFISICFLILIVVGMPIGFVLGSTGLAAMLRMDMTSILQIVP
                     QRYFAGVDMFTLMAMPFFILAGEIMNKSGITGRLVKFANVLVGHLQGGLAHANILASV
                     FFAGITGAAVSDTAAIGSMLIPAMVEEGYDKDFSAAVTASSSIIGPTIPPSNIMVIYG
                     AFMQVSIAGLFFAGVVPGLLVAAALMVLTARISKKRGYPVGERRATFKEMAVAFKEAF
                     VALLMPAIILGGILSGIFTPTEAAS"
BASE COUNT          213 a          311 c          348 g          297 t
ORIGIN      
        1 ggctggtccg aggagctttc gcggtacttg atgatctgga tggcgctgct ctccgtagcc
       61 ctctgtatct ggcgccatga gcacgtcggc gtcacgatgt tcatcaagaa gcttccgcgt
      121 ctgctcgcga aaattatggt cttcgtatcg aacgggttga ttctctactt cctctatgtc
      181 ctggccagat acggcttccg gatggccgag ggaggaaaat ctcaggtttc aacggcgctg
      241 cacacgacga tggagtggtg gctcatggcc gtgccggtga gcgcggtgct ctgcatcatt
      301 atgctcgtct gcaagatgat cctcgatatc cgtcgaaaga atctcgatga gatcctgatg
      361 tcggaggata tcatcgatac agtaaagcgg gaggaaggcc tcgatttcgg ggatgttccc
      421 cttaaggagg aacgatcgtc atgaccggat tcatctcgat ctgttttttg attctcatcg
      481 tggtggggat gcctatcggt tttgtgctgg ggtccactgg tctggcggct atgctcagga
      541 tggatatgac ctccatcctt cagattgttc ctcagcgtta cttcgcgggg gtggacatgt
      601 tcacccttat ggcgatgccg tttttcattt tggcaggaga gatcatgaac aagagcggta
      661 tcacgggccg ccttgtcaag ttcgcaaatg tgcttgtcgg ccatcttcaa ggaggactgg
      721 cgcacgccaa catcctggcg tccgtctttt tcgccgggat cacaggggcg gcggtgagcg
      781 ataccgccgc catcggctcg atgctcatcc cggcgatggt agaggaaggg tacgataagg
      841 acttctccgc ggcggtcact gcgtcgtcat ccatcatcgg tcccaccatt ccgccgtcga
      901 acatcatggt catttacgga gccttcatgc aggtgtccat tgccggactc ttcttcgcgg
      961 gcgtcgttcc cggccttttg gtcgccgcgg cccttatggt tctcacggcg aggatttcaa
     1021 aaaagcgcgg gtatcctgtg ggagagcgcc gggcgacatt caaagagatg gcggtagctt
     1081 tcaaagaagc gttcgtggcg ctgctgatgc ctgccatcat cctggggggt attctctcag
     1141 gcatcttcac gccgaccgag gcagcttcc
//
LOCUS       DAOPKU010000769         1153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_150150_length_1153_cov_3.593807, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000769 DAOPKU010000000
VERSION     DAOPKU010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150150_length_1153_cov_3.593807"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1153)
                     /locus_tag="PK849_15670"
     CDS             complement(<1..>1153)
                     /locus_tag="PK849_15670"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPE67622.1"
                     /translation="TGIDPWFLYQMAEQADFEGILGENVLNGSLLLKAKRFGLSDKRI
                     ARVTGKSESDVEVLREAERIAPSFHFVDTCAGEFPAATPYFYSTWGERDEGDPLGGDA
                     VLILASGPNRIGQGLEFDACCTLASLAWREEGRKTVIVNSNPETVSTDYNVSDRLYIE
                     PLTAEHVKAILKKEGIRDVLVQLGGQTPLNMARELESAGAHIVGTPLSGIEEAEDRGR
                     FAALLDRLGLRQPKNRMAGSREDVAAAAEEIGYPVLLRPSYVIGGKNMFIAYRRDELE
                     EFLDRGIPLSESAPVLVDQFLEDAFEYDLDALCDGTNVYVAGIGEHIEAAGIHSGDSA
                     CVFPVYKSPPEILSEMVAAAVSIAATAFWKSCIFASLESGKGPDAALSRR"
BASE COUNT          209 a          379 c          346 g          219 t
ORIGIN      
        1 gccgtcgtga aagcgctgca tccggacctt tcccggattc gagcgacgcg aagatgcagc
       61 ttttccaaaa tgccgtggcc gcgatggaaa cggcggcggc gaccatctca ctgaggattt
      121 ccggaggcga cttatagacg gggaagacgc aggcgctgtc gcccgagtgg ataccggcgg
      181 cttcgatatg ttcgccgatt ccggcgacgt agacgttcgt tccgtcgcag agcgcgtcga
      241 ggtcgtactc gaaggcgtct tccaggaact gatccaccag tacgggcgcc gactcgctca
      301 acgggattcc tctgtcgagg aactcctcca gctcgtcgcg cctgtaggcg atgaacatgt
      361 tctttccgcc gatgacgtag gaggggcgca gcagcaccgg gtagccgatc tcctccgccg
      421 cggccgcgac gtcctcgcgg gaacccgcca tcctgttttt tggctgacgg agtcccagtc
      481 gatcgagcag cgcggcgaaa cgaccgcggt cctccgcctc ctcgatgccg gagaggggag
      541 ttccgacgat gtgcgcgccc gcagactcca gctcccgggc catgttgagc ggtgtctgcc
      601 ctccgagctg caccaggacg tcgcggatcc cctccttctt cagaatcgcc ttcacgtgct
      661 ccgcggtcag cggttcgatg tacagccggt cggagacgtt gtaatccgtg gagacggtct
      721 cgggattcga gttcacgatc accgtcttcc gcccctcctc ccgccaggcc agcgaagcga
      781 gcgtgcagca ggcgtcgaat tcaagcccct gtccgatccg gttgggtccg gaggcgagga
      841 taagcaccgc gtcgccgccg agggggtcgc cttcgtcgcg ctcgccccat gtcgaataga
      901 aatacggcgt cgcggcgggg aactcccccg cgcaggtatc cacaaagtga aacgacggcg
      961 cgatcctctc ggcttcccga agaacctcca catcagactc gctctttcct gtcacgcggg
     1021 caatacgctt gtcggagaga ccgaagcgtt tagccttcaa cagcaaactt ccgttcagca
     1081 cattttctcc caggatcccc tcgaaatcgg cctgttcagc catctggtac aggaaccacg
     1141 gatcaatgcc tgt
//
LOCUS       DAOPKU010000770         1147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_151419_length_1147_cov_2.345238, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000770 DAOPKU010000000
VERSION     DAOPKU010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151419_length_1147_cov_2.345238"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1147)
                     /locus_tag="PK849_15675"
     CDS             complement(<1..>1147)
                     /locus_tag="PK849_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPE67623.1"
                     /translation="RGIKAIFIYPMNALASDQAQRTARAIWDNPELKERVTAGLYVGH
                     DPEDALDQPVKAMERDKVITCRYTLRENPPDILLTNYKMLDFLLLRPADRELWRYNTP
                     DTLKYLVVDELHTFDGAQGTDLSCLLRRLKSRLDIPSGHLCCVGTSATLGEKERASEV
                     LGYAASVFGERFDADALVTEERLSAGEFLENDMAVRFDLPGPEASGGLDPESFDSPLD
                     YLERQAFLWFGGEGRRKDGESGDEWRVRLGTLLRGHVFFQNFLKVLGGRPMEYSDLLG
                     RLVKVAPRLGEGEDASYAPLVLDSLLSLVSHARKQEEGRLRPLVTVQVQGWLREVRRM
                     VASVPSQGERPELFFSDDLKADRLARSLPAVHCRDCGAMGWASSEGKN"
BASE COUNT          199 a          404 c          354 g          190 t
ORIGIN      
        1 gttctttccc tcggacgagg cccaccccat cgccccgcag tccctgcagt gaaccgcggg
       61 aagggagcgg gcgagccgat ccgccttcag gtcgtcggag aagaagagtt ccggacgctc
      121 gccctgcgaa ggaaccgacg ccaccatccg ccgcacctcc cggagccacc cctgcacctg
      181 caccgtgacc agcggccgga gccgcccctc ctcctgcttt ctcgcgtggc tcacaaggga
      241 gagcaggctg tcgagaacga ggggagcgta ggaagcatcc tccccctctc ccaagcgggg
      301 agcgactttg accagccttc ccagaaggtc ggagtattcc atgggccttc cgccgaggac
      361 cttgaggaag ttctgaaaaa agacgtgccc ccggagcagc gttcccagcc ggactctcca
      421 ctcgtccccg ctctcgccgt ccttcctgcg cccttcgccg ccgaaccaca ggaaagcctg
      481 ccgttcgagg tagtcgaggg gcgaatcgaa ggactcgggg tccagcccgc cgctcgcttc
      541 ggggccgggc agatcgaaac ggacggccat gtcgttctcc agaaactccc ccgcggagag
      601 ccgctcctcc gtaacgaggg cgtcggcgtc gaaccgctcg ccgaaaacgg acgcggcgta
      661 cccgaggact tcggaggcgc gctccttctc cccgagggtg gccgatgtgc cgacgcagca
      721 caggtgtccg gacggaatat caagccgcga tttcagcctc cggagcaggc acgagaggtc
      781 ggtcccctgc gcgccgtcga acgtgtgcag ctcgtccacc accaggtact tcaaagtgtc
      841 gggcgtgttg taacgccaca gctccctgtc ggcggggcga agcagcagaa agtccagcat
      901 cttgtagttg gtcagcagaa tatccggagg attctcccgc agggtgtagc ggcatgtgat
      961 caccttgtcc cgctccatcg ccttgaccgg ctggtcgagc gcgtcctcag ggtcgtgccc
     1021 gacgtacagc ccggcggtca ccctctcctt cagctccgga ttgtcccata tcgcccgcgc
     1081 ggtgcgctgc gcctggtccg acgccagggc gttcatcggg tagatgaaga tcgccttgat
     1141 cccccgg
//
LOCUS       DAOPKU010000771         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_152786_length_1140_cov_4.366820, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000771 DAOPKU010000000
VERSION     DAOPKU010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152786_length_1140_cov_4.366820"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..961
                     /locus_tag="PK849_15680"
     CDS             254..961
                     /locus_tag="PK849_15680"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPE67624.1"
                     /translation="MEPVLEELRKELAAHADEEIRRTGRRFFKEPVNLYGIKTAVGTK
                     IAKEFFKQLKGRTKEEIFSLCEELWKSGMMEESFVACNWSYALRKEFAEEDFLLFERW
                     IQRYVGNWASCDTLCNHTIGAFIETFPQYVDRLKEWTASENRWVRRASAVSLIIPARN
                     GLFLDDVFEIAERLLLDADDLVQKGYGWLLKVTCAKHEDAVFSYILSRKDVMPRTALR
                     YAIEKMPPERKKEAMKR"
BASE COUNT          283 a          268 c          351 g          238 t
ORIGIN      
        1 ggatgatggg gattgctccg aaaccgagtt cggagacgga gacgccggtc ttgccgaaag
       61 gaagatactt catggatgtc agctccttca tgcagattgt ttttccgact ctttcttcgt
      121 caagaatagc agagaaagag ggagtttgaa agagtgccga cggaaagcta ctgggtggag
      181 ccttgatgct tttatttccg cgtggtagag ttttcctgaa aaatgatgac gatcctgaaa
      241 gagagtgatg tctgtggagc cggtgctcga agagcttcga aaagagctcg ccgcccacgc
      301 cgacgaagag atccggcgga ccggacggcg ttttttcaag gaacctgtga atctctacgg
      361 aataaagact gcggtcggaa cgaaaatagc caaggagttt ttcaagcagc tcaaggggag
      421 aacgaaagag gaaatattct ctctctgtga ggagctttgg aaatcgggta tgatggaaga
      481 gtcgttcgtg gcctgcaact ggtcgtacgc gcttcgtaag gaatttgcgg aggaggactt
      541 cctccttttt gagcgatgga ttcagcgtta cgtgggcaac tgggcgtcct gcgataccct
      601 ctgcaaccat acgatcggcg ccttcatcga aacattcccg cagtacgtgg accggctgaa
      661 ggagtggacc gcctcggaga accggtgggt gcgcagagcg tccgcggtct cgctgattat
      721 ccccgccagg aacggcctct tcctcgacga cgtcttcgag atcgccgaac ggttgctgct
      781 cgacgccgac gacctcgtgc agaaggggta cggctggttg ctgaaggtga cctgcgcgaa
      841 gcacgaagac gcagtgttca gctacatcct gagcagaaag gacgtcatgc cgaggactgc
      901 cctgcggtat gcgatagaga agatgcctcc cgagcgaaag aaggaagcca tgaagcgata
      961 gcgtgagaga aggagagaga aatcctgtcc ttgaaacatg ttgtaaaaag atggcgccgc
     1021 ccgtttttac tgttgaacgc cgatcctccg cacatccgac gctccgtgcc ggaatgctgc
     1081 gcggactgaa agagagatga gggaaggatg ataccgaaag aaccgacgta cagtgatctt
//
LOCUS       DAOPKU010000772         1139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_152985_length_1139_cov_4.275830, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000772 DAOPKU010000000
VERSION     DAOPKU010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152985_length_1139_cov_4.275830"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PK849_15685"
     CDS             complement(<1..739)
                     /locus_tag="PK849_15685"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPE67625.1"
                     /translation="MPANLRSIRGVLHFYTLLLAVAMIIFPVLIGVYDLYKASRTVER
                     DIAYALDMQSVALQRWFAIHQNNIASIASLPSVQTKAYDDMFEDFQSFVRTNTNFDSI
                     VYVNTEGRGVVNSTTGFNPRPETDVSDRDYYKDAAKGKPHISDILIGRTSGMPVVIFS
                     APLVGDDGFQGLVFGAIRFDTLLNTILSTHFGATGQFLLLNSKGRSLQGTGESSFPNE
                     TLAALLSSDGYSVSYRDASGKRYLAKGK"
     gene            917..>1139
                     /locus_tag="PK849_15690"
     CDS             917..>1139
                     /locus_tag="PK849_15690"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPE67626.1"
                     /translation="MKRIILCVVAAALFLSCGLTLPAMVSSAAASEIVLTSVGQSPDA
                     TMIRVVLRKMKINAENQPLLRPEGLAGEKI"
BASE COUNT          284 a          267 c          306 g          282 t
ORIGIN      
        1 tcttgccctt ggcgagatat cttttgcccg aagcgtcgcg gtagctcacc gaatatccgt
       61 cggatgagag gagagccgcg agcgtctcgt tgggaaaact gctttctccc gttccctgca
      121 aagatctgcc cttggaattc aggaggagaa actgacctgt cgctccgaag tgtgttgaca
      181 gtatggtatt caaaagggtg tcgaaacgta tggcgccgaa aacaagtccc tggaagccgt
      241 cgtctcccac aagcggagcc gagaaaataa cgacgggcat gcccgatgtc cggccgatga
      301 gaatgtcgga gatatgaggc ttccctttgg cggcgtcctt ataatagtcg cggtcggaaa
      361 catcggtttc ggggcggggg ttgaagcctg tagtgctgtt gacaacgccc cttccttccg
      421 tgttgacata gacgatggaa tcaaagttcg tatttgtacg aacaaaactt tgaaaatctt
      481 cgaacatatc gtcgtatgct tttgtctgga cggacggaag gcttgcgatg gaggcgatgt
      541 tattctggtg tatggcgaac catcgctgga gcgccacaga ctgcatatcc agcgcgtacg
      601 cgatatcccg ctcaacagtc ctcgaggcct tgtagagatc gtacactcct atgagcaccg
      661 gaaagatgat cattgcaact gcaagaagaa gcgtataaaa gtggagcacg ccgcgaatac
      721 ttcgtaaatt agcgggcact tgcatgtacc tcccgtcttc ttttttctat tcaaataata
      781 ccatagggtg ctgtataccg cagccttcga tgtttctatt gaaaagaatg ataagaaata
      841 tggacagaaa actcctgaag ggtacactta aaaagaacgt atgaatcgta tattctattt
      901 ttgaggagga tgtgccatga aaagaataat tctatgcgta gtggcggctg ctctgttcct
      961 ttcctgtggt cttacgcttc cggctatggt ctcttcggcg gcggcttcgg agatcgtgct
     1021 cacctcagta ggacagagtc ctgatgcgac aatgattcgc gtggtgcttc ggaagatgaa
     1081 gatcaacgcg gaaaaccagc ccctgctcag acctgaaggc cttgccggcg agaaaattc
//
LOCUS       DAOPKU010000773         1138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_153192_length_1138_cov_4.716528, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000773 DAOPKU010000000
VERSION     DAOPKU010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153192_length_1138_cov_4.716528"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PK849_15695"
     CDS             <1..538
                     /locus_tag="PK849_15695"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HPE67627.1"
                     /translation="RTWLTDLLPPLRQGELLSLPVEGIPARYDPSLPAGRMNLRWVPL
                     VCEWSSLLEEELESLAAPARERRFGDLLGLGPGSTPAGDDFLAGYTTGRLWLGTPVPP
                     LPDTSRTTWLSAEILRDAADGLVWKRSKDVLESLSLNDAAALLKAVERILGWGHTSGR
                     AWLAGLSAAVSDTIERNV"
     gene            554..>1138
                     /locus_tag="PK849_15700"
     CDS             554..>1138
                     /locus_tag="PK849_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid metabolism regulator protein"
                     /protein_id="HPE67628.1"
                     /translation="MASSQRLEIIRRTYYDSVTLMLVAKELLKLEGISVATLNMGTEA
                     NYKIMGAGGFDLTGVDATPNDLVLALLSDAPEAEKVLDDALAKAKEYLANPPGRKKDS
                     GDNDYRPKSLDGALSVLPEANLAIVSVAGRYAGDVALQCIEKGLNVMLYSDNVTVEKE
                     IEVKKAAAAKGLLVMGPDCGTIIIRGVAMGMANAC"
BASE COUNT          216 a          338 c          375 g          209 t
ORIGIN      
        1 gaggacgtgg ctgacggacc ttcttccccc tctgcggcag ggagagcttc tctccctgcc
       61 ggtggagggc atccctgcgc ggtacgaccc gtcgcttccc gccggacgta tgaaccttcg
      121 atgggttccg ctggtttgcg agtggagttc gctgctcgaa gaggagctgg aatctctcgc
      181 tgcaccggcc cgggaacggc gcttcggcga tcttctcggc ctcgggccgg ggagcacgcc
      241 cgccggggac gacttccttg ccggatacac gaccgggaga ctctggctcg gaactcctgt
      301 tccccctctg ccggatacgt cccggacgac ttggctctcc gctgaaattc tgagggacgc
      361 tgcggacgga ttggtctgga aacgaagcaa agatgtactg gaatctcttt cgctgaacga
      421 cgcggctgcg cttctgaaag cggtcgaacg aatcctcggc tgggggcaca cttccggaag
      481 agcgtggctc gcggggcttt ccgcggccgt ttcggacacg atcgaacgca acgtatgaaa
      541 ggaggatgat actgtggcat cttcccagcg cctcgaaatc atccgaagaa cgtactatga
      601 cagcgtcacc ctgatgcttg tggcgaagga gctgctcaag ctggagggaa tctccgtcgc
      661 cactctgaac atggggacgg aggcgaacta caagattatg ggagccggcg gtttcgacct
      721 gaccggagtg gacgccacgc cgaacgatct tgttctcgct ctgttgagcg acgcgccgga
      781 ggccgaaaag gttctcgacg acgccctcgc gaaggcgaaa gagtaccttg cgaaccctcc
      841 ggggcggaag aaggactcgg gcgacaacga ctacaggccg aagagcctcg acggggcgct
      901 ctcggtcctt cctgaagcca atctcgcgat cgtctcggtc gccgggcgct acgcgggcga
      961 cgttgccctt cagtgcatcg agaaggggct caacgtgatg ctctactccg acaacgtcac
     1021 ggtcgagaag gaaatcgagg tcaagaaggc ggccgccgcg aagggactgc tcgtgatggg
     1081 gcccgactgc ggcacgatca ttatccgcgg cgttgcgatg gggatggcga acgcctgt
//
LOCUS       DAOPKU010000774         1136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_153606_length_1136_cov_4.636448, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000774 DAOPKU010000000
VERSION     DAOPKU010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153606_length_1136_cov_4.636448"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..695
                     /locus_tag="PK849_15705"
     CDS             147..695
                     /locus_tag="PK849_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67629.1"
                     /translation="MREKNRLGLFAGIALLLLLAFSSAALAKPDWVTVKYTGTQLRKQ
                     KNTKVGDGNHLLLVHKFDIINNSKNGDIITAIFDRKIRWNGVFTIVQMTSTTQYFFDG
                     KNSTTWKPAHWNVKFGMTGTNPYKGEWYPGQVYKYEHTIPLNKLIKPYDGSWKTTNEA
                     AFKKFNFKFDKWSLDFQVRSHR"
     gene            807..>1136
                     /locus_tag="PK849_15710"
     CDS             807..>1136
                     /locus_tag="PK849_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67630.1"
                     /translation="MKKTPLLCASALLFVLTCTLPAFATEEQWTTYVNERFGYSVEYA
                     DIYTERDEPENGDGVWLSASEGKYALTLSGGYNVLEEDGERRLRSRLENVSHIVPGSE
                     ESGPDWYR"
BASE COUNT          331 a          264 c          306 g          235 t
ORIGIN      
        1 aggaaaaaat cacaacgcgg gaaataaaaa tagagattcc cgcggcaggg taaagcgtaa
       61 cggaacgcct gtttttgggc gtatttgctg cgggagagga gtttcccgca gcaaatacga
      121 ttttttataa aaatgggggg aaataaatga gagagaagaa caggctcgga ctgtttgcgg
      181 gcatagcgtt gctgctgttg ctggcattct cttccgccgc tctggcaaaa cccgactggg
      241 tgacggtcaa gtacacggga acgcaactgc ggaagcaaaa aaacacgaaa gtcggcgacg
      301 ggaatcacct gcttctggtg cacaaattcg acatcatcaa caacagcaaa aacggcgaca
      361 tcatcacggc gatttttgat cgaaaaattc gctggaacgg tgttttcacg atcgtgcaaa
      421 tgacgagtac tacgcagtat ttttttgacg gcaagaattc tacaacatgg aaaccggcac
      481 actggaacgt aaagttcggc atgaccggga cgaatcccta caaaggcgaa tggtaccccg
      541 gacaggtgta taaatacgaa catacgatac ctctcaataa acttatcaag ccgtatgacg
      601 gcagctggaa gacgaccaac gaagcggcgt tcaaaaaatt caatttcaag ttcgacaaat
      661 ggagtctcga ttttcaggtt cgaagccata ggtagttttg aggttgtatc aaaagagctt
      721 cttccgctgc gctcttcgcc gagaaaacca gtttcgctgg aaaggatgct cccgggcgtc
      781 tcaataatcg agaggagaaa tgaatcttga aaaaaacacc cttgttatgc gcttcggcgc
      841 tgctctttgt tttgacgtgc acgctccccg cttttgcaac cgaggaacag tggacaacct
      901 acgtcaacga gcgcttcggc tactctgtgg aatacgccga tatctacacg gaaagagatg
      961 agccggaaaa cggagacggc gtctggctga gcgcttcgga agggaagtac gcgctgacgc
     1021 tctcgggagg ctacaacgtc cttgaagaag acggagaacg caggctgcgg agcagactcg
     1081 aaaacgtgtc gcatatcgtg cccggctccg aagaaagcgg tcccgactgg tacaga
//
LOCUS       DAOPKU010000775         1136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_153610_length_1136_cov_4.442183, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000775 DAOPKU010000000
VERSION     DAOPKU010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153610_length_1136_cov_4.442183"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1136
                     /locus_tag="PK849_15715"
     CDS             <1..>1136
                     /locus_tag="PK849_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67631.1"
                     /translation="KADTRAPAACHTSFPVELSLDTFSRLQTEAAALDQAAGDPQFTF
                     ILCRAGTNKNGDHFTPDELAARYMTAINKKIDLKHSQDFTDIVGGIVGADYVEDESGG
                     RIECAGELYVSDSPHAQLAYKLIRKGIISQVSMECDYEEGECSICGKRVVSKSDYCVH
                     LRKNKGGELQGQPVYEILHGVTFTGLGLLDRKGADENARILKVAAAENDGSTTHSEGG
                     PTMDEKHKETEEREADAAKKKDDGGGGTAPDDKTRLKELERENKELKQQVLALQKQLE
                     ELEAEKKAAANRSRAQKLLRKIEKSGLAFESDEDREQELSRLAGLSDDAFAASEAAFE
                     RMMKAGSRGHSEDKGAKDRGAGAETTKASCGCDDPPLRSDAGVR"
BASE COUNT          272 a          345 c          370 g          149 t
ORIGIN      
        1 ccaaagcgga tacgcgggca ccggcggcat gtcacacctc gttccctgta gagctgtcgc
       61 tcgatacgtt cagcaggctc cagaccgaag cggcggcatt ggaccaggcc gcgggcgatc
      121 cgcagttcac cttcatcctc tgccgggccg gcacgaacaa gaacggcgac cacttcaccc
      181 ccgacgaact ggccgcccgc tacatgacgg cgatcaacaa gaaaatcgac ctgaagcatt
      241 cccaggattt caccgacatc gtcggcggca tcgtgggggc ggattacgtc gaggacgaaa
      301 gcggcggccg gatcgagtgc gcgggggaac tctatgtctc cgacagcccg catgcgcagt
      361 tggcctacaa gctcattcgc aaagggatca tctcccaggt ctcgatggag tgcgactacg
      421 aggaaggaga gtgctcaatc tgcggcaaac gcgtcgtttc gaagagcgat tactgcgttc
      481 acctgcgcaa gaacaagggc ggtgagctcc aagggcaacc cgtctacgaa atcctccacg
      541 gcgtcacttt caccgggctc gggcttctcg accgcaaagg cgcggacgag aacgcccgca
      601 tcctgaaagt cgccgcggcg gaaaacgatg gttcgacaac ccactctgaa ggaggtccga
      661 caatggacga gaaacacaag gaaaccgagg aacgggaagc ggatgccgcc aagaagaagg
      721 acgacggcgg cggcgggacc gcgcccgatg acaagacccg gctcaaggaa ctggagcggg
      781 agaacaagga actcaaacag caggttctcg cgctgcagaa gcagctcgag gaactggagg
      841 cggagaaaaa ggccgccgca aaccggagcc gagcccagaa gcttctgcgc aagatcgaaa
      901 agagcgggct ggcattcgag agcgacgagg atcgcgaaca ggagctgagc cgtctggccg
      961 gtctttccga cgacgccttc gccgccagcg aagccgcttt cgagcggatg atgaaggccg
     1021 gttcccgcgg tcactccgag gacaagggcg cgaaggaccg aggagcgggc gcggaaacca
     1081 cgaaggcgtc atgcggttgc gacgacccgc ccctgcgcag cgacgcgggc gtccgt
//
LOCUS       DAOPKU010000776         1133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_154277_length_1133_cov_3.350649, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000776 DAOPKU010000000
VERSION     DAOPKU010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154277_length_1133_cov_3.350649"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PK849_15720"
     CDS             <1..337
                     /locus_tag="PK849_15720"
                     /inference="COORDINATES: protein motif:HMM:NF022743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HPE67632.1"
                     /translation="LGVLGVYVIGTLFGAIFMGLFAGFLASTTSLHPYALAMASGVGS
                     GSMMAASSGSLLALFPDMKDQIAAFAGASNLISTGSGLYVSIFVALPLTEWLYKKLEP
                     VLGRKKSVE"
     gene            365..817
                     /locus_tag="PK849_15725"
     CDS             365..817
                     /locus_tag="PK849_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF340 domain-containing protein"
                     /protein_id="HPE67633.1"
                     /translation="MKVSFMDSVVVMIISGVIIAIGNMVGYKVDIMASVPGLVYLFLI
                     ILAGVAMTKIIPWKVPSIAYITLIGILVTIPAFPYSKMIVDATGKINLLATATPVLAY
                     AGIALGKDMETLKKMGWKIVVVSIFVFMGTFIGSAVIAHLILKMQGII"
     gene            complement(911..>1133)
                     /locus_tag="PK849_15730"
     CDS             complement(911..>1133)
                     /locus_tag="PK849_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPE67634.1"
                     /translation="KTVRPNLYIACGISGAIQHLAGMEESDVIVAINKDPAAPIFDVA
                     DFGVVGDALKILPPLTESIKAIRAEKKGA"
BASE COUNT          229 a          347 c          299 g          258 t
ORIGIN      
        1 actgggcgta ctgggcgtct acgtcatcgg aacgctcttc ggcgccatct tcatgggcct
       61 cttcgcaggc ttccttgcct ctacgacatc gctgcacccc tacgccctcg caatggcgtc
      121 cggcgtcggc agcggcagca tgatggcggc ctcctcgggc agtctcctcg cgctcttccc
      181 cgatatgaaa gatcagatcg cggccttcgc cggcgcgtcc aatctcatct ccaccgggag
      241 cggcctctac gtctctatct tcgtcgcgct gcccctgacg gagtggctgt acaagaagct
      301 cgaacccgtc cttggacgga agaagagcgt tgaatagcac caccaggaga ggaggagaaa
      361 cattatgaaa gtttcgttca tggattcagt cgtcgtcatg atcatctcgg gtgttatcat
      421 cgccatcggc aatatggtag ggtacaaggt ggacatcatg gcgtccgttc cggggctcgt
      481 ctacctgttc ctcatcattc tcgcgggtgt ggcgatgaca aagataatcc cctggaaggt
      541 gccgagcatc gcctatatca cactcatcgg tattctggtc accattccgg ctttccccta
      601 ttcgaagatg atcgtcgatg ccacgggcaa gataaacctt ctggccacgg cgacccccgt
      661 gctcgcatac gcgggcatcg cgctgggcaa ggacatggag accctcaaga agatggggtg
      721 gaaaatcgtc gtcgtcagca tcttcgtctt catgggcacg ttcatcggat cggctgttat
      781 cgcccatctc attctgaaaa tgcagggcat aatttagccc gcgattcttc cgcaaacggt
      841 agcgccggcg ttctcgaacg ccggcgctac cgtttgcgca taccgccaaa tcccccgaca
      901 agacggtatg ctatgctcct tttttctccg ccctgatagc cttgatactc tctgttagag
      961 ggggaagaat cttcagcgcg tcgccaacta cgccgaagtc cgcaacgtca aagataggcg
     1021 ctgcgggatc cttgttaatc gccacgatca catcggactc ctccataccg gccagatgct
     1081 gaatcgcccc ggaaatgccg catgcgatat aaagattcgg gcgcaccgtc ttt
//
LOCUS       DAOPKU010000777         1122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_156586_length_1122_cov_3.658857, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000777 DAOPKU010000000
VERSION     DAOPKU010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156586_length_1122_cov_3.658857"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PK849_15735"
     CDS             complement(<1..256)
                     /locus_tag="PK849_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67635.1"
                     /translation="MTESEARTRKERIDARLRSPVLDWIIVPNDEVRDFSSLARHAVE
                     EYPTGTGPADYALFVDGKLLGIIEAKKVSLGAENVLEQAKR"
     gene            complement(546..770)
                     /locus_tag="PK849_15740"
     CDS             complement(546..770)
                     /locus_tag="PK849_15740"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HPE67636.1"
                     /translation="MTKLPVVTGKALIAALEDIGFKVLRIKGSHHYLRHVDGRSTVVP
                     VHAGEEVGPGLLVKILRDCELSKETLENLL"
     gene            complement(767..982)
                     /locus_tag="PK849_15745"
     CDS             complement(767..982)
                     /locus_tag="PK849_15745"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPE67637.1"
                     /translation="MARQFSVIIEQDQDGYYVASVPALRGCHTQAKSLDELSVRVKEA
                     IELCLEVEDNDAERLEFIGVQQVSVPI"
BASE COUNT          250 a          310 c          306 g          256 t
ORIGIN      
        1 atcgtttggc ctgttcgagc acgttttcgg cgccgagaga aacttttttg gcttcgatga
       61 tgccgagcag cttgccgtcg acgaaaaggg cgtagtcggc gggaccggtc ccggtggggt
      121 actcttcgac ggcgtgacgg gcgagcgacg agaagtcgcg gacttcgtcg ttggggacga
      181 tgatccagtc gaggacgggc gatcgaagcc gcgcatcgat gcgctctttt cgtgtacgag
      241 cctcggattc agtcattgcc acaccccttt ttccgtgcgc gccgcggacg ctctcgggcg
      301 gtcaagcaac tcaacagatg cacgcggcgg gaataccacg tgttcttcaa aaaacccgat
      361 cttcgtttta ccatagtcca tctacttcgg caagactttt ccaggattat tttcgaaaag
      421 agcgataaat ttccatgcgt actacttcga cggggggcat agttcattcg gggagaatga
      481 gcaaggggtt atgaggcaac tcgaatcgtc gccggaagtg cgttatggag gaaaacatca
      541 atagctcaaa gcagattttc caaagtctcc ttggagagtt cacaatcccg tagaatcttc
      601 accagaagcc cggggccgac ctcttctccc gcgtgaacag ggacgacggt agaacggccg
      661 tcgacatgcc gcaggtaatg atggctgccc ttgattcgca ggaccttgaa tccgatatct
      721 tcaagtgcgg ctatcaacgc tttccccgtc acgacaggaa gctttgtcat attggaacgc
      781 tcacctgctg gactccgatg aattcgaggc gctccgcatc gttgtcctcc acctcaagac
      841 acagttcgat ggcctctttt acccgtacgg aaagttcgtc gagcgacttc gcctgagtat
      901 gacaccctcg cagtgcaggt actgaagcga cgtaatagcc atcctgatcc tgttcaatga
      961 taacgctgaa ctgcctggcc atgatcccca cctccccgag tatgattcta acagatggac
     1021 agccctgtag aaacggaatc cccctgaagc atgctctggc cggtttctgg tcgcgccgga
     1081 taaccggaga acatcgtatc gtctatcgcg tcgaggacga tg
//
LOCUS       DAOPKU010000778         1113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_158535_length_1113_cov_5.253308, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000778 DAOPKU010000000
VERSION     DAOPKU010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158535_length_1113_cov_5.253308"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1113
                     /locus_tag="PK849_15750"
     CDS             18..>1113
                     /locus_tag="PK849_15750"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPE67638.1"
                     /translation="MLGAIIGDIAGSRFEFNNHRSKEFELFTDKCFATDDSIMTLAVA
                     KAIMEAERSAGSADRDDAFYSALGGLTVRYMREIGQRYPHCGYGGRFHQWMFSPNPRP
                     YNSFGNGAAMRVSPAGFAARTLSEVRRMSEAVTSVTHNHPEGIKGAEATAVAIFMARS
                     GATLEEIRKAVVRDYYPLDFTIDGIRESYQFNETCQETVPQAIEAFLESSSFEDAIRT
                     AISVGGDSDTLAAITGGIAEAYYGVPADLRERALRFLDKDLRAIYDEWVAFTAEEGTV
                     PRFRPLTRHIERLATVKSYGEWIDDVRGDGTSGNPKVLPCMSQHPAATEFVEEFYRFS
                     ATHPEFSLEEYRSILETHGVEWGFDEMRDAD"
BASE COUNT          230 a          342 c          366 g          175 t
ORIGIN      
        1 aaaggaggcg acgccccatg ctcggcgcga tcatcggcga tatcgcgggt tcacgattcg
       61 agttcaacaa ccacaggagc aaggagttcg agctgtttac ggacaagtgt ttcgctacgg
      121 acgacagcat catgacgctc gccgtggcca aggcgatcat ggaggccgag aggagcgccg
      181 gctccgcgga ccgtgacgac gcgttctact ccgctctcgg cggcctgacg gtgaggtaca
      241 tgcgggagat tggccaacgc tatccgcact gcgggtacgg aggacggttc catcaatgga
      301 tgttcagccc gaatccacgg ccctacaaca gcttcggaaa cggcgcggcc atgcgggtaa
      361 gccccgcggg gttcgccgcg cggaccctct ccgaggttcg gcgaatgtcg gaagccgtca
      421 cctcggtgac gcacaaccac ccggagggca tcaagggggc ggaggcgact gcggtcgcaa
      481 tcttcatggc ccgcagcggc gcaacgctgg aggagatacg aaaagccgtc gtccgcgact
      541 actacccgct ggacttcacc atcgacggca tcagggagag ctaccaattc aacgagacgt
      601 gccaggagac ggtcccgcag gcgatcgaag cgtttctgga gtcctcttcg ttcgaggacg
      661 ccatccgcac cgcgatctcc gtcggcggcg acagcgacac tttggcggcc ataacgggcg
      721 gcatcgcgga ggcgtactac ggcgttccgg ccgatctgcg ggagcgggcg ctgcgcttcc
      781 ttgacaagga tctgcgggcg atctacgacg agtgggtcgc attcacggcg gaagagggaa
      841 cggtcccacg cttccggcct ctgaccagac acatcgaaag gctcgccacc gtgaaatcat
      901 acggcgaatg gatcgacgac gtgcggggag acggaacgtc cgggaatccc aaggttttgc
      961 cctgcatgtc gcagcacccc gccgcgacgg aattcgtgga ggagttctac cggttctccg
     1021 cgacgcaccc ggagttcagc ctcgaagagt accggagcat actcgaaaca cacggcgtcg
     1081 aatggggctt cgacgaaatg cgggacgccg acg
//
LOCUS       DAOPKU010000779         1099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_161703_length_1099_cov_5.971264, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000779 DAOPKU010000000
VERSION     DAOPKU010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161703_length_1099_cov_5.971264"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK849_15755"
     CDS             <1..467
                     /locus_tag="PK849_15755"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPE67639.1"
                     /translation="CSEPPCISVAVQKARYSFGGIVARKAFTVNVPSEAQAAEADYFG
                     IFSGRDGDKFAATGLTPRRGEIADAPLVEEFPISFECEVIHSAELGSHVLFVGKVVKT
                     WAFEEHLDERGQPDPLKVRPLVFAPQFGMYYGLRDEPVARAFSVGRTIREGK"
     gene            complement(526..>1099)
                     /locus_tag="PK849_15760"
     CDS             complement(526..>1099)
                     /locus_tag="PK849_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HPE67640.1"
                     /translation="KHLNGGPGAPAGLFVNRRHFGRAPGLAGWFGYVKERQFDMSITF
                     EHAQSAGGWQMGTPCVLSAASLDGALKLFTEAGIGAVREKSLALTGFMIDMVDERLAP
                     LGFEVGTPREPERRGGHVAVEHPEGWRICCALKKKGIIPDFRPPRVIRLAPVALYNTF
                     EDVERTVAALETIVKGREYEAFSAEREAVS"
BASE COUNT          196 a          371 c          339 g          193 t
ORIGIN      
        1 gctgttcgga gcccccctgc atctccgtcg ccgtacagaa ggctcggtac agcttcggcg
       61 gcatcgtagc gcggaaggcg tttaccgtaa acgttccatc cgaggcgcag gccgccgagg
      121 ccgactactt cggcattttt tcggggcgcg acggcgacaa gttcgctgcc accgggctca
      181 cccctcgccg cggagaaatc gcggacgcgc cgctggtgga ggagttccct atctctttcg
      241 agtgcgaagt gattcactcg gccgaacttg gttcccacgt gctcttcgtc ggcaaggtcg
      301 tcaaaacatg ggcgttcgag gagcatctcg acgagagagg acagcccgac ccgctgaaag
      361 tgcgccccct cgtcttcgcg ccccagtttg gaatgtacta cgggctgcgg gacgaacctg
      421 tggcgagagc cttctccgtt ggcagaacca tccgggaggg aaaataaaaa gaaggggccg
      481 agaacggccc cttcgttcat attacgttac cgcagcccct gtcctctaag aaaccgcctc
      541 gcgctcggcg gagaaggctt cgtactcgcg ccccttcacg atcgtctcca gcgcggccac
      601 ggtgcgttcc acgtcctcga acgtgttgta cagcgccacg ggcgccagcc ggatcacgcg
      661 aggagggcgg aagtcgggga tgatcccttt cttcttgagt gcgcagcaga tgcgccaccc
      721 ctccggatgc tccacggcga cgtgtccgcc tctccgttcc ggctcgcggg gcgttcccac
      781 ctcgaagccg aggggggcca gacgctcgtc gaccatgtcg atcataaagc cggtcagcgc
      841 cagcgacttc tcgcgcaccg cgccgatccc cgcctcggtg aagagtttca gcgcgccgtc
      901 gagcgatgcc gcggagagga cgcacggagt ccccatctgc caccccccgg cgctttgagc
      961 atgctcaaag gtgatggaca tgtcgaactg acgttccttc acgtacccga accatccggc
     1021 caaacccgga gcgcgcccga agtgacgccg gttgacgaag aggcccgcag gagcgcccgg
     1081 cccgccgttc aggtgcttg
//
LOCUS       DAOPKU010000780         1099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_161723_length_1099_cov_4.321839, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000780 DAOPKU010000000
VERSION     DAOPKU010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161723_length_1099_cov_4.321839"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PK849_15765"
     CDS             <1..401
                     /locus_tag="PK849_15765"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HPE67641.1"
                     /translation="LALPELRKVWAEIVGGALAAKTYAEDIDEGALLVKAESPAAAKM
                     ISMRAGSLARELSRRTGLSVTSVKVVPGKASHRPSPSRKEEPFRVVPPKELVERNFEE
                     VKEKFSPERETLARRFASLMALFQKRFPGK"
     gene            complement(405..>1099)
                     /locus_tag="PK849_15770"
     CDS             complement(405..>1099)
                     /locus_tag="PK849_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE67642.1"
                     /translation="EATLAENGREAVDILSKKRFGLVLMDIQMPVMDGYEATALIRNP
                     SSDVLDHTVPVVALTAHAVEGFRDECLSRGMDDYLPKPFSSADLLGLLYKWLPMKTRP
                     AAPEPAGAGAQASSPGQDVFDPDGFFSKLFNDREEGKELLDLFLESFPEDIELLRQTI
                     NDGEYDKAAKIAHTMKGTAGNACARSMSQCAKALQLAAKEEDREKTVRLFEELVAIFD
                     KVREAMLKEMER"
BASE COUNT          184 a          362 c          342 g          211 t
ORIGIN      
        1 ggctcgccct cccggagctc cggaaagtat gggcggagat cgtcggaggc gcgctcgcgg
       61 cgaagacgta cgccgaggat atcgacgagg gcgcgctgct ggtgaaggcg gaaagccccg
      121 ccgcggcgaa gatgatctcc atgcgggccg gctcgctcgc gagggagctg tcgaggcgga
      181 cgggactctc cgtgacgtcg gtcaaagtgg ttccgggcaa ggcgtcgcac cgcccgtctc
      241 cgtcccgcaa ggaagagccc ttccgcgttg tcccgccgaa ggagcttgtc gagcgcaatt
      301 tcgaggaggt caaagagaag ttctcccccg agcgggagac gcttgcacgc aggttcgcct
      361 ccttgatggc cctctttcaa aagcgcttcc ccggaaagta gcggtcagcg ctccatctcc
      421 ttcagcatcg cctcgcgaac cttgtcgaag atggcgacca gctcctcgaa cagccgcacg
      481 gtcttctccc ggtcctcttc cttcgccgcc agctgcagcg ccttcgcgca ctggctcatg
      541 ctccgggcgc aggcgtttcc cgccgttccc ttcatcgtgt gggcgatctt cgccgctttg
      601 tcgtactccc cgtcgttgat cgtctgccgc agcagttcga tatcctcagg gaaggattca
      661 aggaaaagat ccaggagctc ctttccttct tcccggtcgt tgaagagttt cgagaagaat
      721 ccgtcggggt cgaacacatc ctgccccggg gacgacgctt gagcccctgc gccggcgggc
      781 tccggcgccg cggggcgcgt cttcatggga agccacttgt agagcaaccc cagcagatcc
      841 gccgaggaga acggtttggg aaggtagtcg tccattcctc tggagagaca ctcgtcgcgg
      901 aaaccttcga cggcgtgggc cgtcagcgcg accacgggga ccgtgtggtc gagcacgtcc
      961 gacgagggat tcctgatcag tgcggtcgct tcgtacccgt ccatgaccgg catctggatg
     1021 tccatcagca ccaggccgaa gcgtttcttc gacagtatat ccacagcctc cctgccgttt
     1081 tccgccagcg tcgcctcgc
//
LOCUS       DAOPKU010000781         1099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_161743_length_1099_cov_3.470307, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000781 DAOPKU010000000
VERSION     DAOPKU010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161743_length_1099_cov_3.470307"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PK849_15775"
     CDS             <1..241
                     /locus_tag="PK849_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPE67643.1"
                     /translation="DPNDAAITRAVVGIARSLGLRVVAEGVETPEQLDFLRRNGCDEI
                     QGYLLSRPLPPDAFEKFGESEIPLCFNGLSGYNHC"
     gene            318..1091
                     /locus_tag="PK849_15780"
     CDS             318..1091
                     /locus_tag="PK849_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE67644.1"
                     /translation="MQHVSSLTLYDQGDHKFIMLGWEEQEEEVAVQTNQYVISSGGEV
                     LLLDPGGAHVFPRVLANVAEMFELKDIRHIFYSHQDPDVSSGITLWLSMAEKATVYIS
                     GLWVRFLPHFGIYDVKRIKAIPDKGDAISLSNGKKMTVIPSHFLHSTGCFSLYDPTSK
                     ILFSGDIGAAIFPRGTRYPVAEDFDAHMKYMEGFHKRYMASNAFCRRWVKTVSALDVE
                     HIAPQHGALIKGRENVKKFLAWFENLACGVDVIDDIYGR"
BASE COUNT          223 a          308 c          333 g          235 t
ORIGIN      
        1 ggatcccaac gacgccgcca tcaccagggc cgtcgtcggc atagcccgtt cgctggggct
       61 gcgcgttgta gcggaagggg tggagactcc ggagcagctt gattttttgc gccgcaacgg
      121 atgcgacgag atccaggggt acttgctgag ccggcctctt ccgccggatg cctttgaaaa
      181 attcggggag agtgaaattc ctctttgttt caacggactc tcaggttata atcactgttg
      241 agacggggtg ctccgtagaa tagaatatac cccggagaaa cgtccgtctc ctctgcatac
      301 ggaaggagtt ggttggagtg cagcacgtga gttcgcttac cctctacgac cagggggatc
      361 acaagttcat catgctcggc tgggaggagc aggaagaaga ggtcgccgtc cagacgaacc
      421 agtacgtcat ctcttccggc ggcgaggtgt tgctgctcga tccgggcgga gcgcacgtct
      481 tcccgagggt gttggcgaat gtggccgaga tgttcgaact gaaggatatc cggcatatct
      541 tctactccca tcaggatccg gacgtctcct cgggcatcac gctctggctg tcgatggccg
      601 agaaggcgac ggtctatatc tccgggctgt gggtgcgctt ccttccgcac ttcggcatct
      661 acgacgtgaa acgcatcaaa gccattcccg acaaggggga tgcgatctcc ctctccaacg
      721 gcaagaagat gacggtcatc ccttctcatt tcctccactc tacaggatgt ttttccctgt
      781 acgacccgac gtcgaagatt ctcttctcgg gcgatatcgg cgcggcgatc ttcccgcgcg
      841 gaacccgcta ccccgtggcg gaggattttg acgcgcacat gaaatacatg gaagggttcc
      901 ataaacgcta catggcatcg aacgccttct gccgcagatg ggtgaagacg gtatcggcgc
      961 tcgacgtgga gcacatcgcc ccgcagcacg gcgcgctgat caaggggcgg gagaacgtga
     1021 agaagttcct cgcgtggttt gaaaacctcg cctgcggcgt agatgtcatt gacgacatct
     1081 acggtcgcta gccggggag
//
LOCUS       DAOPKU010000782         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_161951_length_1098_cov_4.810163, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000782 DAOPKU010000000
VERSION     DAOPKU010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161951_length_1098_cov_4.810163"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1098
                     /locus_tag="PK849_15785"
     CDS             <1..>1098
                     /locus_tag="PK849_15785"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPE67645.1"
                     /translation="ILHVDMTTGTHRMIDDPELFGEFLGGTAVATELLFRHGDPQGDP
                     LAPESPVIFAIGPFSSLFPVATKTVAMFKSPLSGELGESHAGGRMAMSLHEAGIEALV
                     ITGRAEKLSYLVIENDSAEVKSASTFRGQSAAATERILREAEGKSGRKVSILRIGPAG
                     ERLSPMACATVDGSRHFGRLGLGAVLGSKNLKAIVISGTRTLPLARPKEFKAVYDILY
                     DRVVKSKEMKKYHDLGTPANVRPLSLIRGLPTRNFSQGNFEGAEAISGERFAKDFLVQ
                     HTACAHCQCGCIHMAELREEFAPYHYSTNRVSYDYELIYAMGSMLSLSSPEDILKLLL
                     FTEKQGWDAISLGCTLAWATESFLHGVLSLD"
BASE COUNT          204 a          385 c          324 g          185 t
ORIGIN      
        1 cattctccac gtcgacatga cgacgggaac gcaccgcatg atcgacgatc ccgaactctt
       61 cggcgaattt ctgggaggaa ccgccgtcgc cacggaactg ctcttccgcc acggagatcc
      121 gcagggcgac ccgctcgctc ccgagagccc ggttatcttc gcgatcggtc cgttcagctc
      181 gctctttcct gtggcgacga agaccgtcgc gatgttcaaa tcccccctct ccggcgaact
      241 cggggagtcg cacgccggcg gccgcatggc gatgtcgctg cacgaagcgg gaatcgaggc
      301 gctcgtgatc accggcaggg cggaaaaact ctcctacctc gtcattgaga acgactccgc
      361 cgaagtcaag tccgcctcca ccttcagggg gcagtccgcc gccgccaccg agcgtattct
      421 gagagaggct gaggggaaga gcggacgcaa ggtctccatc ctccgcatcg gccccgccgg
      481 cgaacggctc tcacccatgg cgtgcgccac ggtggacggt tcgcggcact tcggcaggct
      541 gggacttggc gcggtcctcg gcagcaagaa cctcaaggcg atcgtcatca gcggaaccag
      601 aacgctcccc ctcgcccgcc cgaaggagtt caaggccgtg tacgacatcc tctacgaccg
      661 ggtggtcaag tcgaaggaga tgaagaagta ccacgatctc ggcaccccgg ccaacgtccg
      721 gccgctctcc ctcatcaggg ggcttccgac gcgcaacttc tcgcagggaa acttcgaggg
      781 ggccgaggcc atctccgggg agcgcttcgc gaaggacttc ctcgtccagc acaccgcctg
      841 cgctcactgc cagtgcggct gcatccacat ggcggagctg cgcgaggagt tcgcccccta
      901 ccactacagc acaaaccgcg tgtcctacga ctacgagctg atctatgcga tggggagcat
      961 gctcagcctc tcctcccccg aggatatcct caagctgctg ctcttcaccg agaagcaggg
     1021 gtgggatgcg atatcgctcg gctgtaccct cgcctgggcc acggaatcct tcctccacgg
     1081 cgtcctctcg ctcgacga
//
LOCUS       DAOPKU010000783         1096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_162560_length_1096_cov_1.737752, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000783 DAOPKU010000000
VERSION     DAOPKU010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162560_length_1096_cov_1.737752"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1096)
                     /locus_tag="PK849_15790"
     CDS             complement(<1..>1096)
                     /locus_tag="PK849_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE67646.1"
                     /translation="DVQDHPVFTDKPLFVGDIVGAVLAKDEETARTAAAAVKIGFEEE
                     APLLDMEQAALPQTPSFREEMPPVIEGKIAYGEESLPGDAIILESTVRTQKIHHGAME
                     NHICEARMEYGGVLLVETPCQMAFTVRYVLADLFDIPFNKVRVVKTPVGGAFGGKQEV
                     GIEPHCALMALKTGLPVRLALDRTQTILATRTRAASVGRILIAADREGNLLRREMDVI
                     VDAGAYATGTQRVMMAMGKKTSRLYRIPSQSFRGRTVFTNTTPAGACRGYGSPQIHAI
                     TEIAMDLLARKLSMDPAELRLKNLVHPWDTDPAGGPALGNARIRDCLVRGMESFRWKE
                     RRVIPPGERRFRRGVGLACCTHGNGYFGSPY"
BASE COUNT          195 a          344 c          341 g          216 t
ORIGIN      
        1 gatacggcga gccgaagtag ccgttgccgt gcgtgcagca cgcgagtccg acgccgcgcc
       61 ggaagcgtct ttcgccgggt ggaatcacgc gccgttcctt ccacctgaag gactccatcc
      121 cacgaacaag acagtcgcgg atacgcgcat ttccgagagc gggcccgccc gccgggtccg
      181 tatcccacgg atggacaagg tttttcaatc tgagttccgc cgggtccatt gaaagctttc
      241 gggcgagcag gtccatcgca atctccgtga tcgcgtgaat ctgcggggaa ccgtaccctc
      301 ggcatgcgcc cgcaggggtg gtgttcgtga aaacagtgcg tccccggaac gactgggacg
      361 gaatacggta gagccgggag gtcttcttcc ccatcgccat cattacccgc tgcgtccccg
      421 tggcataggc gccagcgtcc acgatcacgt ccatctcacg ccggagaagg ttcccttctc
      481 tgtccgcggc gatgagaatc cgtccgacgg aggcagccct ggtcctcgtc gcgaggatgg
      541 tctgcgtacg gtcgagagca agccgaacgg ggagtcccgt cttcagcgcc atcagagcgc
      601 agtgcggttc tatgccgact tcctgcttgc cgccgaatgc tccgccgacc ggagtcttca
      661 ccacgcgaac tttattgaac gggatgtcga aaaggtcggc caggacgtag cggacggtga
      721 acgccatttg gcacggagtt tcaacaagga ggacgccgcc gtactccatc cgtgcctcgc
      781 agatgtggtt ttccatcgcg ccgtggtgta ttttctgcgt ccggacggtg ctttcgagaa
      841 tgatcgcatc cccgggaaga ctctcctcgc cgtaggcgat tttgccttcg atcaccgggg
      901 gcatttcctc gcgaaacgag ggcgtctgcg ggagcgcggc ctgctccata tcgagcagag
      961 gcgcctcttc ttcaaaaccg atcttgacgg cggcggccgc tgtgcgagcg gtttcctcgt
     1021 ccttggcgag caccgcgccg acaatgtcgc cgacgaagag cggtttgtcc gtgaagacgg
     1081 ggtgatcctg cacatc
//
LOCUS       DAOPKU010000784         1087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_164577_length_1087_cov_2.986434, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000784 DAOPKU010000000
VERSION     DAOPKU010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164577_length_1087_cov_2.986434"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PK849_15795"
     CDS             <1..631
                     /locus_tag="PK849_15795"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE67647.1"
                     /translation="WALLMPVIILGGIYGGIFTPTEASAVAAVYGAFIGMFVYKDLKL
                     KDFPKVLLSAATSTTMIMYVVGAANLFGWILTNAQVPHRIAAGFTAMTDSPFVFLILV
                     NLLLLFIGTLLNASAAVVILTPILLPVALSLGIDPVFFGVLMVVNLAIGCITPPVGLD
                     LFVVSSITKISIDKIMKDITPYLLALLVVLLILTWFPDIILFLPRLLRG"
     gene            703..>1087
                     /locus_tag="PK849_15800"
     CDS             703..>1087
                     /locus_tag="PK849_15800"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HPE67648.1"
                     /translation="MREIAEKLARAMEASKQTVVFTGAGMSTESGLPDFRSSNGLWGK
                     YRPENLASVDALNRNFENFAAFYRQRIATLGEVRPNKGHLILAEWEKRGLVSAIITQN
                     VDGLHHAAGSRVVHELHGTLREVRCQ"
BASE COUNT          195 a          360 c          305 g          227 t
ORIGIN      
        1 ctgggcgctc ctgatgccgg tgatcatcct cggcggcatc tacggcggca tctttacccc
       61 taccgaagcg tccgcagtcg cggcggttta cggcgcgttc atcggaatgt tcgtctataa
      121 ggatctgaag ctgaaggact tcccgaaggt actcctgagc gccgccacca gcacaacaat
      181 gatcatgtac gtcgtcggcg cggcgaacct cttcggctgg atcctcacca acgcgcaggt
      241 accgcacagg atcgccgcgg gcttcaccgc gatgacggac agtccgttcg tcttcctcat
      301 cctcgtgaac ctccttctgc tcttcatcgg caccctcctg aacgcctcag cggcggttgt
      361 catccttacg ccgatcctgc tcccggtcgc actctccctc gggatagatc cggtcttctt
      421 cggcgtcctg atggtcgtga acctcgccat cggctgcatc acgcctcccg tcggactgga
      481 cctcttcgtc gtgtcgagca tcacgaaaat ttccattgac aagatcatga aggacattac
      541 gccgtacctt ctcgcgctgc tcgtggtact gctgatactc acctggttcc cggacattat
      601 tctcttcctc cccaggcttc tgagggggta gacatcggcc cgcccggcgg cgccccgaag
      661 gcgctccgcc gggcggattt ctttctcaag gaggggttcg atatgaggga aatagcggaa
      721 aaactcgccc gtgcgatgga ggcgtcgaag cagacggtcg tcttcaccgg agccgggatg
      781 agcaccgaat ccggtcttcc cgacttccgt tcatcgaacg gtctctgggg aaagtaccgc
      841 cccgagaacc tcgcttcggt ggacgcgctc aatcgcaact tcgagaactt cgccgccttt
      901 taccgtcagc gcatcgcgac cctcggggag gtacgcccga acaagggaca ccttattctg
      961 gcggagtggg agaaacgagg gcttgtctcg gcgatcatca cgcagaacgt ggacggcctg
     1021 caccacgccg ccggttcccg cgtcgttcac gaactccacg ggacgctccg ggaagtgcgc
     1081 tgccaga
//
LOCUS       DAOPKU010000785         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_165931_length_1081_cov_4.710526, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000785 DAOPKU010000000
VERSION     DAOPKU010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165931_length_1081_cov_4.710526"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PK849_15805"
     CDS             <1..864
                     /locus_tag="PK849_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPE67649.1"
                     /translation="DLVALLHTEMKHLLPGIRGGSEETTSGVKRLRSMANEGILAFPM
                     ISVNDAHSKYLFDNRYGTGQSVMDGVMRTTNMLVAGKTVVIAGYGWCGRGVAMRAAAM
                     GARVLVVEIDPHRAFEALMDGYEVAGMAEAASRGDIFLTLTGNTKVIRKEHFEKMKDG
                     VLLGNAGHFDVEISKPDLDSLTESVEETRPGIRTYVLKDGRRLHLLGEGRLVNLACAD
                     GHPIEIMDLSFALQLESAIYMAKNSMKPGMYDVPAEIDRSVMEAKLASMNITLDTMTP
                     EQEAYMKSWVE"
     gene            866..>1081
                     /locus_tag="PK849_15810"
     CDS             866..>1081
                     /locus_tag="PK849_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE67650.1"
                     /translation="MATLFKNFLAVDGEMAEGRYCSLRVEDGRITAVADKLAALPGDE
                     VVDGERRVAIIPGFVNAHTHAAMSLLRG"
BASE COUNT          210 a          297 c          390 g          184 t
ORIGIN      
        1 gaccttgtgg cgctgctgca taccgagatg aagcatctgc ttcccggtat ccgtgggggc
       61 tccgaggaga cgacgtccgg agtgaagcgc ctgcgttcga tggcgaacga gggaattctg
      121 gccttcccga tgatctcggt gaacgacgcg cacagcaagt atctcttcga caaccgctac
      181 ggtacggggc agtcggtgat ggacggcgtc atgcggacga cgaacatgct ggtcgcgggg
      241 aagaccgtgg tcatcgcggg atacggctgg tgcgggcgcg gcgtggcgat gcgcgcggcg
      301 gcgatgggcg cgcgggtgct tgtggtggag atcgatccgc accgcgcctt cgaggcgctg
      361 atggacggct acgaagtcgc cggaatggcg gaggccgctt cgcggggcga tatcttcctg
      421 acgctcaccg gcaacacgaa ggtgatccgc aaggagcact tcgagaagat gaaggacggc
      481 gtgctgctgg ggaacgcggg gcacttcgac gtggaaatca gcaaaccgga tttggacagc
      541 ctgacggagt ccgtcgagga gacgcgcccc ggcatacgga cgtatgtgct gaaggacggc
      601 cggagactgc accttctcgg cgaaggacgt ctggtgaacc ttgcgtgcgc cgacgggcac
      661 cctatcgaaa ttatggatct gagcttcgcg ctgcagcttg aatccgccat ctacatggcg
      721 aaaaacagca tgaaacctgg gatgtacgac gttcctgcgg agatcgaccg gtcggtgatg
      781 gaggcgaagc tggcctcgat gaacatcacg ctcgacacga tgactcccga gcaggaagcg
      841 tacatgaaga gctgggtgga gtagcatggc gaccctcttc aagaacttcc tggccgtcga
      901 cggcgagatg gcggaggggc gctactgctc tctccgcgtc gaggacggca gaataacggc
      961 cgtcgcggac aagctggcgg cgcttcccgg cgacgaggtg gtcgacggcg agcggcgggt
     1021 ggcgatcatt ccgggttttg tcaacgcgca tacccacgcc gcgatgtcgc tgctgcgggg
     1081 g
//
LOCUS       DAOPKU010000786         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_165944_length_1081_cov_3.874269, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000786 DAOPKU010000000
VERSION     DAOPKU010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165944_length_1081_cov_3.874269"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1081)
                     /gene="istA"
                     /locus_tag="PK849_15815"
     CDS             complement(<1..>1081)
                     /gene="istA"
                     /locus_tag="PK849_15815"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HPE67651.1"
                     /translation="LGKAREHGVTWSKAEGMTNGELRELFFPGVAVPFSRTRPDCERI
                     HREMGKSGVTLTLLWQEYCEECRQNGDIPLMYSQFCLCYQRYARGANATMRMVRKPGE
                     SAEVDWAGQKGFWLDPETGERFEASIFVGVLSSSRYAYVEAFPSQNQESWLNAHANMF
                     RYFGGVPRILIPDNLKTGVTKPDRYEPVVNRLYRELAEHYGTAVIPARVGKPRDKANA
                     EGTVGAITTWILAALRERNFFSLRELNEGIREKLDRFNAKPFQKKEGSRLDAFLAEEK
                     DLLLPLPKNPYELSSWKKVTADKSYHVLVEKMLYSVPYEYIGHEMEVRLTRNVVEVFF
                     EGQRICSHARIFGRIGQCVTVPSHMP"
BASE COUNT          171 a          342 c          326 g          242 t
ORIGIN      
        1 ggcatgtgcg acgggaccgt gacgcactgt ccgatacggc cgaaaatccg ggcgtgggag
       61 catatcctct ggccttcgaa gaagacctcc acgacgttcc tcgtcaggcg tacctccatt
      121 tcgtgtccga tgtactcgta cgggacgctg tagagcatct tctcgaccag tacgtggtac
      181 gacttgtccg ccgtgacctt cttccacgac gagagttcgt acggattctt cggcagcggc
      241 aggagaaggt ctttctcctc ggcgagaaag gcgtcgagcc tgctcccctc cttcttctgg
      301 aagggtttgg cgttgaagcg gtcgagcttc tcgcgtatcc cctcgttcag ctcccgcagg
      361 ctgaagaagt ttctttcgcg gagcgcggcc agtatccatg tggtgatcgc tccgacggtt
      421 ccttcggcgt tcgccttgtc cctgggcttt ccaacccgtg cggggatgac cgccgtgccg
      481 tagtgctccg ccagctccct gtaaagacga ttgactacgg gttcgtatct gtccggcttg
      541 gtgactccgg tcttgaggtt gtcgggtatc aggatgcggg gcacgccgcc gaagtaccgg
      601 aacatgttcg cgtgcgcgtt cagccagctc tcctgattct gcgagggaaa ggcctcgacg
      661 taggcgtacc ggctgctgga gaggacgcct acgaagatgg acgcctcgaa acgctccccc
      721 gtctccggat cgagccagaa ccccttctgt cccgcccagt cgacttccgc gctctcgccg
      781 ggtttgcgga ccattcgcat cgtcgcgttc gcgccacgtg cgtatctctg gtagcagaga
      841 cagaactggg agtacatcag cgggatgtcg ccgttctgcc tgcactcttc gcagtactcc
      901 tgccacagga gcgtcagcgt gacgccgctt ttgcccatct ccctgtgaat gcgctcgcag
      961 tccggcctgg tgcgggaaaa ggggacggcg acgccgggga agaagagttc gcgcagctct
     1021 ccgttcgtca tgccttccgc cttgctccac gtcactccgt gttcgcgggc cttcccgagt
     1081 a
//
LOCUS       DAOPKU010000787         1080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_166254_length_1080_cov_2.782439, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000787 DAOPKU010000000
VERSION     DAOPKU010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166254_length_1080_cov_2.782439"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PK849_15820"
     CDS             complement(<1..826)
                     /locus_tag="PK849_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67652.1"
                     /translation="MIYQAVSWEGSLMGPETLEKLAEEALPGQRPGDFGLDGRVREEI
                     LETWASARAQWELFKGRRDRDDPKDKYGTSRTRQFWIQPLLALLGYDLRNTPAVEIGG
                     RSFAVSHRAAGRICPVHIVGCFESLDRKREGSKSSPHSMTQEYVNLSETLFALLTNGL
                     QLRLVRDSSRLIKLSYLEFNLERIFEDELFTDFALLFRLLHSSRWAAEGENPAESLLE
                     TYHQDSLENGSRIREKLSEAVVDALETWAEGLLIHPDNSDFRTSLREGRTTPRQLYD"
     gene            complement(823..>1080)
                     /locus_tag="PK849_15825"
     CDS             complement(823..>1080)
                     /locus_tag="PK849_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67653.1"
                     /translation="EKARQDSTDLAAQKTQLERELSEIENLRPSFDALALQRAGELIR
                     QHERYYKALGAESKSERFRVVEPVIPMDILGIYIFLPGGAS"
BASE COUNT          167 a          325 c          358 g          230 t
ORIGIN      
        1 gatcgtagag ttgacgcggg gtcgtacgcc cctcccgcag ggaggtgcgg aagtcgctgt
       61 tgtccggatg gatcagcagt ccttccgccc atgtttcgag ggcgtcgacg acggcttcgg
      121 agagcttctc cctgatgcgg ctaccgtttt cgagactgtc ctgatggtac gtttcgagaa
      181 gcgattccgc ggggttctcc ccctcggcgg cccatctgct gctatggagc aggcggaaga
      241 gcagggcgaa gtcggtgaac agctcgtcct cgaagatgcg ttcgaggttg aattcgaggt
      301 acgagagctt gatcaggcgg ctcgagtcgc ggacgaggcg aagctgaagt ccgttggtca
      361 gcagtgcgaa gagcgtctcg gagaggttga cgtactcctg cgtcatgctg tgggggctgc
      421 tcttgctccc ctcccgcttt ctgtcgaggc tttcgaagca gccgacgatg tgcaccgggc
      481 agatcctccc ggcggcgcgg tggctgacgg cgaagcttct accgccgatc tcgacggcgg
      541 gggtgttccg gaggtcgtag ccgaggagcg cgaggagcgg ctgaatccag aactggcgcg
      601 tccggctggt gccgtacttg tccttggggt cgtcccggtc gcgccggccc ttgaacagct
      661 cccactgcgc ccttgcgctc gcccacgtct caagaatctc ctcgcgcacc ctgccgtcga
      721 gtccgaagtc gccgggtcgc tgtcctggaa gcgcctcctc cgcgagcttt tcgagtgttt
      781 ccggccccat cagggaacct tcccacgaga cggcctggta gatcatgacg cacctccggg
      841 gaggaagatg tagattccaa ggatgtccat cgggatcacg ggttcgacca cacggaagcg
      901 ctcgcttttc gattcggctc cgagcgcctt gtagtagcgc tcgtgctgcc ggatgagttc
      961 tcccgcacgt tgcaacgcca atgcgtcgaa ggagggacgc agattctcga tttcggagag
     1021 ttctctttcg agctgcgtct tctgcgcggc gaggtccgtg gagtcctgtc gcgccttctc
//
LOCUS       DAOPKU010000788         1067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_169333_length_1067_cov_3.379447, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000788 DAOPKU010000000
VERSION     DAOPKU010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169333_length_1067_cov_3.379447"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PK849_15830"
     CDS             <1..557
                     /locus_tag="PK849_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE67654.1"
                     /translation="ETHAELIVDGIFNHDALIYCSYALVFFLCWFFNSTRQGLYLRAT
                     GDNPRAADAMGISVAKTRYFYTMVGAGIVAVGGAYMSVAYTKMWAEHMTAGRGWIAVA
                     LVIFAIWNPARAAFGAYLFGGVEAFQLRLQAAGTTIPAPILMMLPYLMTIGVLLFISI
                     KKGKGIVFGAPASLGLPFFREERD"
     gene            666..>1067
                     /locus_tag="PK849_15835"
     CDS             666..>1067
                     /locus_tag="PK849_15835"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HPE67655.1"
                     /translation="MDFFFQHIPVSGSILNAAAIALGGLAGLLLHSRLSQRFITITFQ
                     GLGLFTIFLGFLMAQRTQSVLIMVFSIVGGSLLGEWFDLDKRLNNLAEWTKGKIRSKN
                     EKFTEGFVAASLLFCVGSMAILGAIEEGMGEY"
BASE COUNT          199 a          319 c          296 g          253 t
ORIGIN      
        1 atgaaacgca tgcggaactc atcgtagatg gcatcttcaa tcacgatgcg ctcatctact
       61 gctcgtacgc cctcgtcttc tttctctgct ggttctttaa ctccacaagg caggggctgt
      121 atctccgcgc caccggagac aaccctcgag ccgccgacgc gatgggcatc tccgtcgcga
      181 agacgcgcta cttctacacg atggttggag cgggtatcgt agctgtgggc ggcgcgtaca
      241 tgtccgtggc ctatacgaag atgtgggcgg agcacatgac cgccggacgc ggatggattg
      301 cagtcgcgct cgtcatcttc gccatctgga accctgcgcg cgcagccttc ggcgcctatc
      361 ttttcggcgg tgtggaagcc ttccagctca gacttcaggc tgcgggaacc actatccctg
      421 cgccaatcct gatgatgctc ccctacctga tgacgatcgg cgtccttctc ttcatctcca
      481 ttaagaaagg gaaagggatc gtcttcggag cgcctgcctc cctgggactt ccttttttcc
      541 gggaggaacg cgactaactg gtttcccgaa cgcacacacg ctcatcgggc gtcggcgatc
      601 gatacggcgc ccgatgacga cggccgccgg atatactctt tcaccataca ggaggttgtt
      661 ccaaagtgga ttttttcttt cagcacatcc ccgtttcggg gagcattctc aatgcggcgg
      721 caatcgctct cggcggcctc gccggtcttt tgctccattc ccgtctttcg caacggttta
      781 tcaccattac ttttcaggga ctgggactct tcacgatctt tctcgggttt ctgatggcgc
      841 agaggacgca gagcgtcctc atcatggtct tcagcatcgt cggcggctcg cttctcggcg
      901 aatggttcga cctggacaaa cgtctgaaca acctggcgga gtggaccaag ggaaagatac
      961 gctcgaaaaa cgaaaagttc acagaggggt tcgtcgccgc ttcgcttctc ttctgcgtcg
     1021 gctcgatggc gatcctcggc gccatcgaag aggggatggg agagtat
//
LOCUS       DAOPKU010000789         1067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_169371_length_1067_cov_2.850791, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000789 DAOPKU010000000
VERSION     DAOPKU010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169371_length_1067_cov_2.850791"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..290)
                     /locus_tag="PK849_15840"
     CDS             complement(90..290)
                     /locus_tag="PK849_15840"
                     /inference="COORDINATES: protein motif:HMM:NF018249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67656.1"
                     /translation="MAKADPERVIILRSVSNFAMQWKGASAYESLMSEGGDEYSSVEA
                     AVRNVHAVGAAVLRELLKERTP"
     gene            complement(350..>1067)
                     /locus_tag="PK849_15845"
     CDS             complement(350..>1067)
                     /locus_tag="PK849_15845"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPE67657.1"
                     /translation="TEKHGWDAISLGCTLAWATESFLHGVLSLDDTGGVSLSFGDCGA
                     YLEVLERMAKGESEFYRDLEMGCAFCAKKRGGLEWAIQYGGVEPGGYMTGENFAVTCL
                     LGVRHSHLDDNGYSIDQNLLNAPKPLEEQVRAQVKEARWRMVLNSLVICLFARGVYDE
                     EIILQGLDALGAEWDAGKLRELAAKALLRKHEWKRLCGFDPATLVVPEKMYTVQTSTG
                     MIDRENLQQRIHLYLEEIRR"
BASE COUNT          179 a          355 c          309 g          224 t
ORIGIN      
        1 cttctccccc tgccgggata aagcagaaga gaggcttccc gtccgggagg cctctctcga
       61 atttccgtat gcctgcatgc gccgttccgt cagggcgtcc gctccttcag cagctcgcgc
      121 aacacagcgg cgccgacggc gtggacgttg cggactgccg cttcgaccga gctgtattcg
      181 tcgcccccct cgctcatcag gctctcgtag gccgaagccc ccttccactg catggcgaag
      241 ttggaaaccg acctgaggat gatcacccgc tccggatcgg cctttgccag ttgagagagg
      301 ctggcgcctt cgtcttcccg cgtgctcagg tcgacgatag cctctgcgct tacctcctta
      361 tttcttcaag atagagatga atacgttgtt gcaggttttc tctgtcgatc attccggtgc
      421 ttgtctgtac cgtgtacatc ttctccggca cgacgagcgt agcgggatcg aatccgcaca
      481 gccgtttcca ctcgtgcttc cgcagcagcg ctttcgccgc cagttcccgg agtttcccgg
      541 cgtcccactc cgcgccgagg gcgtcgagcc cttgaaggat aatctcctca tcatagacgc
      601 cgcgcgcgaa gaggcagatc accagcgagt tgagcaccat gcgccagcgc gcctccttca
      661 cctgggcgcg tacctgctcc tccaagggtt tgggcgcgtt cagcagattc tggtcgatgg
      721 agtagccgtt gtcgtcgaga tgggagtggc gcacgccgag gaggcaggtg accgcaaagt
      781 tctcgcccgt catgtagccg ccgggctcca cgccgccgta ttggatggcc cattcaagcc
      841 cgccgcgctt cttcgcgcag aacgcgcacc ccatttcaag gtcgcggtag aactcgctct
      901 ctcctttcgc catccgttcg aggacttcga gatacgctcc gcagtcgccg aaggagagcg
      961 aaacgccgcc tgtatcgtcg agcgagagca ctccgtggag gaaggactcc gtagcccagg
     1021 cgagggtgca gccgagcgat attgcgtccc acccatgctt ctcggtg
//
LOCUS       DAOPKU010000790         1063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_170395_length_1063_cov_2.563492, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000790 DAOPKU010000000
VERSION     DAOPKU010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170395_length_1063_cov_2.563492"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PK849_15850"
     CDS             complement(<1..409)
                     /locus_tag="PK849_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67658.1"
                     /translation="MEYLADLADTLAMLQEEKDAIAALGEEAGNGAAVMKRNYDTWRK
                     AISEYDRLKKSLVRGYSYFYGKRRVPGWLVIKSDADSVQAPDRTIPEEFGPELGTLQR
                     MKTSIASDLRRLPQVKGDERRFRSTLEKIKKTYN"
     gene            complement(424..>1063)
                     /locus_tag="PK849_15855"
     CDS             complement(424..>1063)
                     /locus_tag="PK849_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67659.1"
                     /translation="SLAVKMKIAVEASLKGTAYGTVKGAFELAKVSGKWAGGAIVGGA
                     ETVIFLAGEALDTLNTLEVSGATLREQGMAQEVQDAKSVSSGRELAGELRRMASLAAS
                     QRSLLEQNIRWARSLDIHRSRALDDLRSRLEEAAQVSLPDVEQRAEEAAKRYPPEFKR
                     LAEYSAVIHSKIKAVEKELAGGKSALASLETSSSSLRSTAGISTASTRSPRE"
BASE COUNT          161 a          358 c          296 g          248 t
ORIGIN      
        1 cgttatacgt ctttttgatt ttctcaagcg tcgaacggaa acgccgctcg tcccccttca
       61 cctgaggaag ccgtcgcagg tccgaggcga tggaggtttt catcctctgc agcgttccga
      121 gttcgggccc gaactcctcg gggatcgtcc tgtcgggagc ctggaccgag tccgcgtcgc
      181 tcttgatcac caaccatccg ggaacgcgcc tctttccgta gaagtaggag tagcccctga
      241 caagggattt tttcaacctg tcgtattcgg agatcgcctt tctccaggta tcgtagttcc
      301 gtttcataac ggcggcgccg ttccccgctt cttcccccag ggccgcgatc gcgtccttct
      361 cctcttgcag catagcgagc gtatccgcga ggtcggccag gtactccatc cgtcggagct
      421 ccttcattcc ctcggcgagc gcgttgaagc cgtcgagatt cctgccgtgc tccgcaacga
      481 cgaacttgat gtctccagcg acgcgagggc ggactttcct ccggcaagct ccttctccac
      541 agctttgatt ttggagtgga tgaccgcgct gtattccgcg agacgcttga attcgggagg
      601 gtatcgcttc gccgcctctt cagcgcgctg ttccacatcc gggagcgaga cctgtgcggc
      661 ttcttccagc ctgcttcgca ggtcgtccag cgctctgctc ctgtggatgt cgaggctgcg
      721 ggcccagcgg atattctgtt ccaacagcga ccgctgcgac gccgcaaggg atgccatgcg
      781 ccggagttcg ccggcgagtt ctctgcctga ggagaccgac ttcgcgtcct gcacctcttg
      841 ggccatcccc tgctcgcgaa gcgtcgcgcc gctgacttca agagtgttca gggtgtcgag
      901 agcctctccc gcaaggaaga tcaccgtctc cgcgccgccg acgatggccc ctcccgccca
      961 ttttccgctc acctttgcga gttcgaaggc gcccttgact gttccgtagg cggttccctt
     1021 cagcgacgcc tctacggcga tcttcatctt caccgcaagg ctc
//
LOCUS       DAOPKU010000791         1061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_170846_length_1061_cov_3.126243, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000791 DAOPKU010000000
VERSION     DAOPKU010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170846_length_1061_cov_3.126243"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PK849_15860"
     CDS             complement(<1..938)
                     /locus_tag="PK849_15860"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HPE67660.1"
                     /translation="MAVTPDLGKFIPVRTEDLVDVLCEEQTLSAEDREKFRRVAELVE
                     ATYHYDFHSTLKEARGAYHIFNPDADTLKICPPGESMDARFDSMVKALEEILVAANYR
                     EFPIAELNELMTKAAPKRLNVRVNLENYEKILIYRRGTKQVQKPKEPLSWWKSLRKKD
                     APVEPEMEEMIQRLLILIKLKSQEDMEQFYDTFIKTAAETENERERRMTREAVLGKKE
                     KEKEKETAAKKSNGAPKVPTIFLKVFKNVPASTLQTLFPDVTIQMTLIDKGKIALPII
                     AGFLSVVNRVIPALLVVGTLIAAIVAGRNIDWAHFK"
BASE COUNT          180 a          331 c          300 g          250 t
ORIGIN      
        1 tccttgaagt gcgcccagtc gatgttccgt ccggcgacga tcgcggcgat cagcgtgccg
       61 acgacgagca gcgcggggat gacgcggttc accacgctca aaaaacccgc gatgatcggc
      121 agggcgatct ttcccttgtc gatcagggtc atctggatgg tcacgtcggg gaagagcgtc
      181 tgcagcgtcg aggccggaac gttcttgaag accttcagga agatcgtagg caccttcggc
      241 gcgccgttgc tcttcttcgc agccgtttcc ttctccttct ccttctcctt tttgccgagc
      301 accgcctcgc gggtcatgcg gcgctcgcgc tcgttctcgg tctccgcggc ggtcttgatg
      361 aaggtgtcgt agaactgctc catgtcctcc tgactcttca gcttgatcag gatgagcagc
      421 cgctggatca tctcctccat ctccggttca acgggcgcgt ccttcttccg gagcgacttc
      481 caccaggaga gcggttcctt cggtttttga acctgcttcg ttccccgccg gtagatcagt
      541 atcttctcgt agttctcaag gttgactcgc acgttcagcc tcttgggggc cgccttcgtc
      601 atcagttcgt tcagctcggc gatgggaaac tcgcgatagt tcgccgctac aaggatttct
      661 tcgagcgcct tcaccatgct gtcgaagcgg gcgtccatgc tttcgccggg cggacagatc
      721 ttcagggtgt cggcgtcggg gttgaagata tggtaggcgc cgcgcgcctc tttcagcgta
      781 ctgtgaaagt cgtagtggta cgtcgcctcg accagttcgg cgacgcggcg gaacttctcc
      841 ctgtcctccg ccgaaagggt ctgctcctcg cacagaacgt ccacaaggtc ctcggtgcgc
      901 acggggatga acttgcccag atccggagta accgccatcg caacccctcc tgtattaaaa
      961 agttcgacgc gtcctctgcg gggaagagcc cgcccgaaga atccgtcgtc ggaaacaaac
     1021 cgtctccaca tagtacattc atccggcctg tttatcaata g
//
LOCUS       DAOPKU010000792         1058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_171569_length_1058_cov_3.449651, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000792 DAOPKU010000000
VERSION     DAOPKU010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171569_length_1058_cov_3.449651"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1030
                     /locus_tag="PK849_15865"
     CDS             200..1030
                     /locus_tag="PK849_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HPE67661.1"
                     /translation="MNVKKRVWEIVEVAKPGDTVSRVFDIFILTLIIVNVISVIIGSV
                     NQIYDRWGRALDLFEFVSVVIFTGEYVARLWACTVDPRYSGAVRGRIRFALRPMSIID
                     LLAILPFYLPFWSVDLRSMRVLRLLRILRIAKAGRYFTSFGLIKDVLYTNKEELILTT
                     AMMGLLLVVSSSILYYCENEAQPEAFASIPHTMWWAIATLTTVGYGDIAPITGIGRTF
                     ASIIAVIGIGMFALPAGILGAGFVEAIHRRKKREEAAQKKEEDEERGNYCPHCGKRLR
                     "
BASE COUNT          220 a          344 c          287 g          207 t
ORIGIN      
        1 caggtcccat cccgccgaga acccccagaa ccgtatccgg tctgcgtatg gtcgtcataa
       61 aaacacctcc ggaaaatatg ctccgtgtat tatacatggt atgcgttttt caggaagagg
      121 gagcaagaaa aaactctcct tgactccttt cgcgcacatg ctgtatcttt ggcgcacttc
      181 gggagaggag aggatcgcag tgaacgtcaa aaagcgggtc tgggaaatag tcgaagtcgc
      241 caagccgggc gataccgtca gcagggtgtt cgacatcttt attcttacgc tcatcatcgt
      301 caacgtgatc tccgtcatca tcggctccgt caaccagatc tacgacaggt ggggaagggc
      361 gctcgacctg ttcgaattcg tctcggtcgt catcttcacc ggggagtacg tcgccagact
      421 gtgggcctgc accgtcgacc cccgctactc cggcgccgtc cggggacgca tccgctttgc
      481 gctgcgcccc atgtccatca tcgacctgct cgcaatcctt cccttctacc tccccttctg
      541 gagcgtcgac cttcgctcca tgcgcgtgct gcgcctcctc cgcatcctgc ggatcgccaa
      601 ggcggggcgc tacttcacat cgttcggcct catcaaggac gtgctgtaca cgaacaaaga
      661 agaactcatc ctcaccaccg ccatgatggg cctgcttctc gtcgtctcgt cgagcatcct
      721 ctactactgc gaaaacgagg cccagccgga agccttcgcc agcattcccc acacaatgtg
      781 gtgggccatc gcaacgctca ccaccgtcgg atacggcgac atcgccccga tcacaggcat
      841 cggaagaacc ttcgccagca tcatcgccgt catcggcatc ggcatgttcg ctctccccgc
      901 gggcatcctg ggcgcgggat tcgtcgaggc catccatcgg cgcaagaagc gtgaagaggc
      961 cgcgcagaaa aaagaggagg acgaagagcg gggcaactac tgcccgcact gcggcaagcg
     1021 cctgcggtga ggcgggaaag ccggctacgc tgatcagg
//
LOCUS       DAOPKU010000793         1056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_172071_length_1056_cov_4.086913, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000793 DAOPKU010000000
VERSION     DAOPKU010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172071_length_1056_cov_4.086913"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1056)
                     /locus_tag="PK849_15870"
     CDS             complement(<1..>1056)
                     /locus_tag="PK849_15870"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPE67662.1"
                     /translation="TQIPLSVRTGVPLKNASGAVVGALSAGISFSREVFVEQAKEMFG
                     AEMTIFAGDVRLMTTIVQDGKRVVGTKLDPAIAAIVLGGASYNGAANILGAPYVTAYD
                     PIEGPDGKVVGIYFSGIPMTEVNAARWAVVRTVLLLAGALLVLGVLLQGWFVRKIVRS
                     IRTMMGFMKEAETGTLCYTREDFGIRSRDEIGDMADALGSMMEKLCTVIGELRGDAQG
                     TAQRAQALAALSEESLASMEEIGASVERANALSEHSASALEETGAGVHEVAESASAAA
                     RSSEEGAEAAGQTKEISERAVGEVEHSITLIREGGRKVNQTAREMENVSRSVESISGF
                     VATITSIADQTNLLALN"
BASE COUNT          188 a          368 c          309 g          191 t
ORIGIN      
        1 cgttcagcgc cagcagattc gtctgatcgg cgatggacgt gatggtggcg acaaaaccgg
       61 agatggactc caccgaacgg gagacgttct ccatctccct cgcggtctgg ttgacctttc
      121 ggcccccctc ccggatgagg gtgatggaat gttcaacctc tccgacggcg cgctcgctga
      181 tctccttcgt ctgccccgca gcctcggcgc cctcttccga ggaacgcgcc gccgcggaag
      241 cgctctcggc tacctcgtga acgcccgcgc ccgtctcctc aagcgcggaa gcgctgtgtt
      301 cggagagagc gttcgcgcgc tcgacggagg cccctatctc ctccatcgac gcaaggctct
      361 cctcggagag cgcggcaagc gcctgcgccc tctgagcagt accctgcgca tccccgcgaa
      421 gctcgccgat gactgtacaa agcttctcca tcatagaacc gagggcgtcc gccatgtcgc
      481 cgatttcatc ccgcgagcgg atgccgaagt cctcccgggt gtaacagagt gtcccggttt
      541 ccgcttcctt catgaatccc atcatagtgc ggatgctccg gacgatcttt ctgacgaacc
      601 acccttgaag cagcacgccg agcacgagga gcgctccggc aagaagcagc acggtacgga
      661 cgaccgccca ccgggcggcg ttcacctcgg tcatcgggat gccggagaag tagatgccga
      721 ctacctttcc gtccgggccc tcaatagggt cgtacgcagt cacgtaggga gcgccgagga
      781 tattcgccgc gccgttgtag ctcgctcctc cgagcacgat ggccgctatg gcgggatcga
      841 gcttcgtccc caccacgcgc ttgccgtcct gcacgatggt cgtcatcagg cggacgtcgc
      901 ccgcaaatat ggtcatctcc gcgccgaaca tttccttcgc ctgctcgacg aaaacctctc
      961 tcgaaaagga gatccctgcg gagagcgcgc cgacgacggc gccggaggca ttcttcaacg
     1021 gcacgccggt gcgcaccgaa agcggaatct gcgtcc
//
LOCUS       DAOPKU010000794         1054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_172564_length_1054_cov_5.192192, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000794 DAOPKU010000000
VERSION     DAOPKU010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172564_length_1054_cov_5.192192"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PK849_15875"
     CDS             complement(<1..696)
                     /locus_tag="PK849_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPE67663.1"
                     /translation="MDAQEDRTKSSAESGKDRHPHAGAGKISLKEALGGEATDVHLFP
                     AVSSTPEDAPLSGAKARVRIGGVLGPLTAVDADVDGELLALLNNPPKPDFSTGLPSGR
                     RLRVAIRTVCSGIAREIFIRVLPETIPPVFGKLAHLLPELASVTPGLVLVSGITGSGK
                     STLLASVAMRHLELGAHVVTVEDPVEYLLHSKTTTGYASQREVGLDVPDFASGVKMSL
                     REDPDTIIVGEIRD"
BASE COUNT          203 a          348 c          308 g          195 t
ORIGIN      
        1 gtctcgaatc tccccgacga tgatcgtgtc aggatcttcg cgcagggaca tcttcacccc
       61 ggaggcgaag tccggaacgt cgagcccgac ctcccgctgc gacgcgtacc ccgtggttgt
      121 tttcgagtgg agaaggtatt ccaccggatc ctccaccgtc accacgtgcg ccccaagctc
      181 cagatgccgc atggccacgg acgcgagcag cgtcgacttg ccgctccccg tgatccccga
      241 gaccagcacc agcccgggag tcaccgaagc gagttccgga agcaggtggg caagcttgcc
      301 gaacaccggg gggatagtct ccggcagcac gcggatgaag atctccctcg cgataccgga
      361 acatactgta cgaatcgcca cgcggagccg ccttccggac ggcagaccgg tcgagaagtc
      421 gggtttcggc gggttgttga gcagcgcgag cagctcgccg tcgacatccg cgtccaccgc
      481 cgtcaggggc cccagcacgc ccccgatgcg caccctcgcc ttcgctccgc tgagcggagc
      541 gtcctcgggc gtagaagaca ccgcagggaa gagatggaca tccgtcgcct ccccgccgag
      601 cgcctctttc agcgaaattt tccctgcgcc tgcatgagga tggcgatctt tcccgctttc
      661 ggcactgctc ttcgttctgt cttcctgtgc atccatcggc gttcaccttg ttttgtatac
      721 gacatatacg gtccattagc agtaccaact tttcaccgct ttctgcggaa tagtgtatca
      781 tcaatccgag gcgtagagag cctgaccatg cgttcattca gcatcccgtt ttttggaacg
      841 cccggtgacg gaagagacgg agtgccgcgc ttccgaaacg acggacagcc agggaggaaa
      901 agcgtgtgaa caaccaaaac atccgcgccg tcgccgttgg cgcagccaat atcgatgtcc
      961 acggctttcc aggtcagccg ctggtcatgc gggattcgaa tcccggacac atccggacct
     1021 gccccggcgg ggtggcgcgg aacatcgcgg agaa
//
LOCUS       DAOPKU010000795         1047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_174347_length_1047_cov_4.609879, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000795 DAOPKU010000000
VERSION     DAOPKU010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174347_length_1047_cov_4.609879"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PK849_15880"
     CDS             <1..345
                     /locus_tag="PK849_15880"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE67664.1"
                     /translation="EGARPWGGYELLHQGERFAIKRVTVLPGKRLSLQYHHHRSEHWV
                     VVRGAALVTLDGEERYVNEGESVFIPKHCVHRLENPGKVMLELVEVRGGEYIEEDDIV
                     RIEDDYGRTTAR"
     gene            381..>1047
                     /locus_tag="PK849_15885"
     CDS             381..>1047
                     /locus_tag="PK849_15885"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HPE67665.1"
                     /translation="MSAKRFFLLVAALLVVLGGAADGEARLSRTLLDLGYDREARLDG
                     ADAAFSAFFPMPAMSRMSGEMLWKIAPSAALLGNGTFSFFLNDRLILTRSVEALRKEP
                     LVRLPLPADLPRDRFLHARIESRIGITDDVCRDLFSGQLFWTVREDSGFSLDDLRIPH
                     RSVSDFFETLYGSLSILLPASPSREEAEAAVWLAAMLRKAVPGLSVSLDMDDNVSSAS
                     ASFI"
BASE COUNT          189 a          288 c          346 g          224 t
ORIGIN      
        1 gagggcgcgc gtccgtgggg agggtacgag ctgctgcacc agggggagcg cttcgcgatc
       61 aagagggtca cggtccttcc cggcaaacgt ctgagcctcc agtaccatca ccaccgcagc
      121 gagcattggg tggtcgtccg cggcgccgcg ctggtgacgc tcgacggcga ggaacgctac
      181 gtcaacgaag gagagagcgt cttcattccg aagcactgcg tgcacaggct ggagaacccc
      241 gggaaggtca tgctggaact ggtggaggtg cgcggaggag agtacattga agaggacgat
      301 atcgtccgca tcgaggacga ttacggaagg acgaccgcgc gatgaagcgg cggaaacagg
      361 atttccggga ggcgatacga ttgagtgcga aacggttctt tctgcttgta gctgcgctgc
      421 tggttgttct gggaggagcg gccgacggag aggcgcgtct ttcacggacg cttttggatc
      481 tggggtatga cagggaggcg cgtctggacg gagcggacgc cgcattctcc gcgttctttc
      541 cgatgccggc gatgagcagg atgtccgggg agatgctctg gaagatagcc ccctctgcgg
      601 cgttactcgg gaacggtacg ttctccttct ttttaaacga cagactgatt ctgaccagaa
      661 gcgtcgaggc gcttcgcaaa gagccgctgg ttcgtcttcc gcttcccgca gaccttcccc
      721 gcgaccgttt tctgcacgcg cgtatcgagt ccaggatagg tataacggac gatgtgtgcc
      781 gggatctctt ttcggggcag cttttctgga ctgtgcggga ggattcgggg ttctctctcg
      841 acgacttgag gataccgcac cgctccgtct ccgacttctt cgagacgctc tacggttcgc
      901 tcagtattct gctcccggcc agtccttcaa gagaagaagc cgaagcggcc gtctggcttg
      961 ccgcgatgct caggaaggcc gtcccggggc tttccgtttc tttggatatg gacgacaacg
     1021 tgtcctctgc ctccgcttcg tttatca
//
LOCUS       DAOPKU010000796         1047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_174348_length_1047_cov_4.590726, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000796 DAOPKU010000000
VERSION     DAOPKU010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174348_length_1047_cov_4.590726"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..591
                     /locus_tag="PK849_15890"
     CDS             268..591
                     /locus_tag="PK849_15890"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPE67666.1"
                     /translation="MNALQPVEDKRSIIERLRRERTALASLGVLEIGLFGSFARGEQT
                     PESDVDLLVEFTPQQHTFDSFMDLSFFLEALLGRKVELLTRESLSPYIGERILAEVEK
                     IHGAA"
     gene            complement(824..>1047)
                     /locus_tag="PK849_15895"
     CDS             complement(824..>1047)
                     /locus_tag="PK849_15895"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HPE67667.1"
                     /translation="TRPSGRCHTRNSIGRMKRKDLERLLRMAGCYLKREGASHALWIN
                     PKTGTMEAVPRHAEIKEPLARKILKSLDA"
BASE COUNT          230 a          272 c          285 g          260 t
ORIGIN      
        1 gtggatatag gtcatcgttc ggcgatctat tcccgaagga gataggctgc gtacttctgc
       61 gggcgatgga ggacgctctt cgtgacgtga tagggagtcc gaacagcatc tctcttacct
      121 ctcttgagaa atttgtgcag ctggagctgt ttcggcgcct ttcgtctatc agaaaaagag
      181 acgtgagttt gccatttttt cccgcttcgg cgacggtgct atactctttg tattgaccgg
      241 tgagatctgg agaaggagat gatcggaatg aacgcgctgc agcctgtgga ggataaacga
      301 tccattatcg aacgcctgcg gagggagcga actgctctcg cctccttggg cgttctggag
      361 atcgggctgt tcggctcctt cgcgcggggc gaacagactc cggagagcga cgtcgacctt
      421 cttgtcgaat tcacccccca acagcatacg ttcgattcgt tcatggatct ctctttcttt
      481 ctggaggctc ttctcggaag aaaagtggag ttgctgaccc gtgaatcgct cagtccatac
      541 atcggcgaac gcattcttgc ggaggtcgaa aaaatacatg gcgcagcgtg aatatccgat
      601 agtccgggat gtcgccgtca ataagctccc cggactgaga gaacagcttc gccgttttgt
      661 gaacacgtga gacttcgaca ggaggagtaa tcatcggtga atatcttcaa aggggagggg
      721 caaactgctt cccccctttc tttagaaagt ggaaacacca gcgaatcgga gcgtgctatg
      781 aacggctttt cggagatcga gatcccggaa tcgccattaa atctcacgcg tcgaggctct
      841 ttagtatttt cctcgccaac ggttctttga tctccgcatg ccgaggaaca gcttccatcg
      901 taccggtttt cgggttgatc cacagagcat gcgaagcgcc ctcgcgtttc aaataacacc
      961 ccgccatgcg aagcaagcgt tccaaatcct ttcgcttcat ccgaccgata ctgtttcgcg
     1021 tatgacatcg tccggaaggc cgcgtag
//
LOCUS       DAOPKU010000797         1047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_174366_length_1047_cov_3.761089, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000797 DAOPKU010000000
VERSION     DAOPKU010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174366_length_1047_cov_3.761089"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1047
                     /locus_tag="PK849_15900"
     CDS             <1..>1047
                     /locus_tag="PK849_15900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE67668.1"
                     /translation="KAKEVFLANVSHEVRTPLNGIVGMTTLLEETPLDARQRELLGVL
                     KDESGALLRLINDLLDFSKMEAGKLVFEDAPFDLYELWNSVRDSFLPRAVAKNLSFSM
                     TLDESAPRVVSGDSFRIRQVLGNLVGNAVKFTGAGSVEVNGNCLARREESFFFEVTVR
                     DTGIGISTKKKDELFQPFVQGDVSMTRKYGGTGLGLAISKRIVDGMGGRITLESREGE
                     GSLFSFTIPMREVRVPEGPFPQHLREKEAREDTYASASILVVEDNPTNRTVAVALLNL
                     LGLQNIETAENGEEAIEKLSATQYDLVFMDIQMPLLDGLQALAKIRDPSSKVVDHETP
                     VVAMTANALPGERE"
BASE COUNT          245 a          254 c          303 g          245 t
ORIGIN      
        1 tgaaggcaaa agaggtcttt cttgcgaatg tcagtcatga agtgcgtacg ccgctcaacg
       61 gtattgtcgg tatgacgaca ctgctggagg aaacgccttt ggatgcgcgc cagagagagc
      121 tccttggtgt tctgaaagac gaatcaggag ctcttctccg gctgatcaac gatcttctgg
      181 atttttcaaa gatggaggcg ggaaaacttg tcttcgaaga cgctcccttc gacctgtacg
      241 agctttggaa cagcgtccgc gacagtttcc ttccgcgcgc cgtcgcaaaa aacctctcgt
      301 tttcgatgac gctcgacgaa tcggcgcccc gagttgtatc cggcgactcc ttcaggatac
      361 gccaggtcct cgggaatctc gtggggaatg cagtaaaatt taccggggca ggatctgtcg
      421 aagtcaacgg taactgcttg gcacggaggg aagaatcctt tttttttgaa gttacagtcc
      481 gagatacggg catagggatt tccacgaaaa agaaggacga gcttttccag ccttttgtcc
      541 agggtgacgt ttcaatgacc cgaaagtatg gcggcacggg gctcggtttg gctatttcga
      601 aacgcatcgt cgacggtatg ggaggccgga ttacgctgga gagccgcgag ggggagggct
      661 ccctcttttc cttcactatt cccatgcgcg aggtccgagt tcccgaaggg ccctttccgc
      721 agcacttgcg ggaaaaggag gcgcgagaag atacgtatgc gtcggcttcc atactcgttg
      781 tggaggataa cccgacaaac aggaccgtgg cggtcgcact gctcaatttg cttggtctgc
      841 agaatatcga aacggctgaa aacggtgaag aagctattga aaagctctct gcaacacaat
      901 atgatttagt cttcatggat attcagatgc cgttgctcga tggacttcag gcgcttgcca
      961 aaattcggga tccatcgtcc aaggtagtgg atcacgagac tcctgtggta gcgatgacgg
     1021 caaatgctct ccctggggaa agagagc
//
LOCUS       DAOPKU010000798         1043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_175405_length_1043_cov_3.707490, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000798 DAOPKU010000000
VERSION     DAOPKU010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175405_length_1043_cov_3.707490"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..>1043
                     /locus_tag="PK849_15905"
     CDS             286..>1043
                     /locus_tag="PK849_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE67669.1"
                     /translation="MKRYGKLVCIAIAALLAFAFLGGAAFAKELVVYSSVDEENAKNL
                     LDEFSKATGITVNMVFLSSGPAMSRIEAEQANPQADVWFGAPNENHIIAKDRGLTQPY
                     VSAAAGDLASGFKDPEGYWHAFYMNPLGFGVLPEELKADGKPVPASWKDLLDPAYKGM
                     IQMPSPQSSGTAYAMIMTLIETFGEDEAYAYMKELNPNIQTYTQSGTGPSKNLAIRET
                     SIAIQFTPAFLKLVDEGYPAKVVFPAAGVGYEAAA"
BASE COUNT          219 a          325 c          301 g          198 t
ORIGIN      
        1 gacaacggtt ttcggttttc aaggtcctgc gaaagttgag gcattataaa caagacatct
       61 gaggatgaca acggaaacac acaggtgttt cacgatgatc atctttaatt cctcgctcca
      121 caagcagaga gcgtatctta gcctctcccc tacgttatca cacccaaccg acaccatacg
      181 tcgttctccc ccccgctccg cgccccttgc tttgtgttac aatactgcaa gatttccgca
      241 acactttgag cagtcctaca ctctgattcg ggagggattt gaagaatgaa acggtacgga
      301 aaactggtgt gcatcgcgat tgcggcgctt ctggcgttcg cgttcctcgg cggcgcggcg
      361 ttcgcgaagg aactggtggt ctactccagc gtggacgagg agaatgcgaa gaacctcctc
      421 gacgagttct cgaaggcgac cggcatcacg gtgaacatgg tctttctctc ctccggtccg
      481 gcgatgagcc gcatcgaggc cgagcaggcc aatccgcagg cggacgtctg gttcggcgct
      541 cccaacgaaa accacatcat cgcaaaggac cgcggcctga cgcagccgta cgtctccgcg
      601 gcggcgggcg accttgcttc cggcttcaag gatcccgagg ggtactggca cgcgttctac
      661 atgaatccgc tcggcttcgg cgtgctcccc gaggagctga aagccgacgg caagcccgtt
      721 ccggcgtcgt ggaaggatct gcttgacccg gcgtacaagg ggatgattca gatgccctcg
      781 ccgcagtcgt ccggcacggc ctacgccatg atcatgaccc tgatcgagac gttcggcgag
      841 gacgaggcct acgcgtacat gaaggaactc aatccgaaca tccagacgta cacgcagagc
      901 ggcaccggcc ccagcaagaa cctcgccatc cgcgagacgt ccatcgccat ccagttcacc
      961 ccggcgttcc tgaagctggt ggacgagggg tacccggcga aagtcgtctt ccccgccgcg
     1021 ggcgtggggt acgaggcggc ggc
//
LOCUS       DAOPKU010000799         1043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_175424_length_1043_cov_3.382591, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000799 DAOPKU010000000
VERSION     DAOPKU010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175424_length_1043_cov_3.382591"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PK849_15910"
     CDS             complement(<1..754)
                     /locus_tag="PK849_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67670.1"
                     /translation="MYKGTSGVCMKVMSVLILAVVVAALPALAQERQERTVETLMDYF
                     DSLVKQGMEATETLLKSKKREKSEVEAVWDDLNERFISVYSKVGHFEFDSMLKKERNE
                     ARASRASEWDNSMKLFIHDLQKVGEVVAWQRKVNRDIAQLEYDIEKAPKRVEAYSGKL
                     KEEGPRVEKLLHELKSGSLSSAELQQRLDEVKNLRAEAENVSNSLERTLTRLGEDLLL
                     KPEMDKRIARILEDWSNMKARHPSVADELTDMP"
BASE COUNT          194 a          326 c          237 g          286 t
ORIGIN      
        1 caggcatatc cgttagctcg tccgcaacgg aggggtgtcg ggctttcata ttcgaccagt
       61 cctccaatat ccgggctatc ctcttgtcca tctccggctt cagcagaagg tcttcaccga
      121 gacgcgtgag cgtgcgttcg agactgttcg agacgttttc ggcctccgct ctcaggtttt
      181 tcacttcgtc gaggcgttgc tgaagctccg cgctcgaaag ggaaccgctt ttcaactcat
      241 ggaggagctt ctcgacgcgc ggcccctctt ccttcaattt tcccgagtac gcctccaccc
      301 ttttcggcgc tttttctata tcgtactcaa gttgagcaat atcccgattt acctttcgct
      361 gccatgccac gacctcccct accttctgaa gatcgtggat gaagagcttc atgctgttgt
      421 cccactcgga agctcgcgac gccctcgctt cgtttcgttc ttttttcagc atgctgtcga
      481 attcgaaatg gccgaccttg ctgtacacgg agatgaagcg ttcgttgaga tcgtcccaca
      541 ctgcttcgac ctccgatttt tccctcttct tcgatttcag aagcgtttcc gtcgcctcca
      601 tgccctgctt gacaagagaa tcgaaatagt ccatcagcgt ctccacagtc cgctcctgcc
      661 gttcctgggc cagggcggga agagcggcaa cgacgaccgc gagaatcaga acgctcatca
      721 ccttcatgca cacgcccgat gtgcccttgt acatgggtca ttcctcctct acggcaaaaa
      781 acccgccgtc aagtcttcgc cagcgtaatc tccttgagcg ccggtggacg accaagcgtc
      841 ttgcgcctgg cggtatgctt ttccaagata actttcgctg tttttcgcgc atcttccagt
      901 ccttttctcc gcgatcgcta tacaaagttc gcagagtacg cattgtttct tcataccgag
      961 cttcaccttc tctcccgaat attagaaggg ctgctcgtag ttatcgatat accatcgccc
     1021 cttgtccctt cggataagga agt
//
LOCUS       DAOPKU010000800         1037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_177044_length_1037_cov_2.774949, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000800 DAOPKU010000000
VERSION     DAOPKU010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177044_length_1037_cov_2.774949"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PK849_15915"
     CDS             <1..895
                     /locus_tag="PK849_15915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPE67671.1"
                     /translation="AAVVSKVPGRTVLRVHVNEGDYVKKGQKLAEVDDSLLRQQIAQA
                     EAVLGKARTYAATIGAEYKRIAALYQENVVSRQQYERMDGEAKVAVRQVEEARAGVNQ
                     LRIMLGYHTLEAPVSGVVLSRAVDPGDTVSQSPAFIISRQENVTITGGVPERSYPSVR
                     VGQKVFVTLDAFPGERFEGVVLRIAPALDPGTRTGKVEVLLPSGGRIKPGMFARVSLK
                     LGERQGYAVPLEAVGRMSGSGESICYVVSGDAALLRKISTGAEKGGQVEILSGLEPDE
                     IIIATRSEKIRDGVQVKVSDR"
BASE COUNT          202 a          284 c          358 g          193 t
ORIGIN      
        1 ggccgctgtt gtgagcaagg ttcccgggcg cacggtgctt cgggtgcatg tgaacgaagg
       61 agattatgtg aagaagggac aaaaacttgc cgaagtggac gacagccttc tgcgtcagca
      121 gatcgcgcag gccgaggccg tgctggggaa ggcgcggacg tacgccgcga cgatcggagc
      181 ggagtacaaa aggatcgccg ctctctatca ggagaacgtc gtcagcaggc agcagtacga
      241 gaggatggac ggagaggcga aggttgctgt gcgccaggtg gaggaggccc gggccggggt
      301 gaaccagctt cggatcatgc tcggctacca tacgctggag gcgcctgtat cgggagttgt
      361 gctctcgcgg gcagtcgatc cgggggacac tgtctcgcag tcgcctgctt ttatcatctc
      421 aaggcaggag aacgtgacga ttaccggagg cgtacccgag cgttcctacc cgtcggtacg
      481 ggtcggtcag aaggtcttcg tcactcttga cgcattcccc ggggagcgct ttgaaggagt
      541 cgtcctgcgt atcgcacccg ctctcgatcc cgggacgcgc acgggaaaag tggaagtgct
      601 gcttccctcg ggaggccgca tcaagcccgg catgttcgcc cgcgtctcgc tcaagttggg
      661 cgaacgccaa gggtacgccg tgccgcttga agcggtaggg agaatgtccg gctcggggga
      721 gagcatctgt tacgtggtct ccggggacgc ggcgctgttg cggaaaattt ccacgggcgc
      781 tgaaaaaggc ggacaggttg agattctgag cgggctcgaa cccgacgaaa taatcatcgc
      841 cactcgttcc gagaagatcc gcgacggcgt tcaggtgaag gtgagcgatc gatgaacctc
      901 ccggagcttt ccgtcaagcg caagacggcc atgctgatgg tctttctcgc ggcgatcctc
      961 ctgggaggcg tcgtcttcgt cgggttgaag ctcgacttgc tccccaggat cgaaccgccc
     1021 gtggtgaaca tcctgac
//
LOCUS       DAOPKU010000801         1035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_177552_length_1035_cov_2.439796, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000801 DAOPKU010000000
VERSION     DAOPKU010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177552_length_1035_cov_2.439796"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PK849_15920"
     CDS             <1..965
                     /locus_tag="PK849_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67672.1"
                     /translation="GCSKEELEEGVNLQRNRIEKVLRLLSVESPSPVVKNGSKWSATA
                     AAQNYVVDKEHIQAITAIRRAEQKEMRRYMEHTECLMEFLERALDDPAPQPCGKCVNC
                     DPNSFLFARCDEEILRKATLFLRNEQHTILPRKKWPMGHPLPSHGFSGRIPEEIRFSE
                     GRVLSLWGDSGLGRLVAEGKYITGRFSDELVEACCEMIRLWKPEPAPQWVACVPSLSR
                     PELVPDFAERLAGALGIPFSACIEKVRSNQPQKEMQNSFQQARNLDGVFTFAREKKVY
                     KPCLLVDDMVDSRWTFTVLSALLRMNGVQAVHPLALALTASGDA"
BASE COUNT          232 a          257 c          316 g          230 t
ORIGIN      
        1 taggctgttc caaagaggag ctggaagaag gggtgaatct gcaaagaaac cggatagaaa
       61 aagtattacg gttactatcc gtggaatctc cgtcgcctgt tgtgaaaaac ggatcaaaat
      121 ggtccgctac tgccgctgcg cagaattatg ttgtggataa agagcatata caggcgatta
      181 cggccatccg aagagcggag cagaaggaga tgcgccgcta tatggagcac acggagtgcc
      241 tgatggagtt cctcgaaaga gcactcgacg atccggcacc acagccctgc gggaaatgcg
      301 tcaattgcga cccgaactct tttctattcg ctcggtgcga cgaagagatt ctccggaagg
      361 cgactctctt tctgaggaat gagcagcata cgattcttcc ccggaaaaaa tggccgatgg
      421 gacatcctct cccgtcgcac ggtttttcgg gcagaatccc cgaagagatt cgtttttcag
      481 aggggcgcgt gctttcgctc tggggcgact ccggactggg gcgacttgtt gcggaaggga
      541 agtacataac gggaagattt tcggacgaac tggttgaagc ctgctgcgag atgatccgcc
      601 tctggaagcc ggaacctgcg ccgcagtggg tggcgtgcgt gccttcattg tcccgccccg
      661 agcttgtgcc ggatttcgcg gagcgtcttg ccggggcgtt gggtattccc ttcagcgcgt
      721 gcattgagaa agtgcgatcc aatcaaccgc agaaggaaat gcagaacagc ttccagcagg
      781 cccgcaatct tgacggagtc ttcacgttcg ccagagagaa gaaggtatat aaaccgtgtc
      841 tgctggtgga cgacatggtg gattcgcgat ggacctttac cgttctctcc gcgcttttga
      901 ggatgaacgg agtgcaggcg gttcatccgc ttgctcttgc ccttactgcg tcgggggatg
      961 cttgagccat gacgtatcgt atgagcgacg acacgaaggc gattctgctt ctctgcggcg
     1021 ttttcggcga cagga
//
LOCUS       DAOPKU010000802         1032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_178246_length_1032_cov_4.500512, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000802 DAOPKU010000000
VERSION     DAOPKU010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178246_length_1032_cov_4.500512"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PK849_15925"
     CDS             <1..539
                     /locus_tag="PK849_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67673.1"
                     /translation="EALAAEALLESNTVSNARATVDVSFSPEQLSPVQAEVESPRYSI
                     RAWIAAYAGADGKYPEIGLHLGRNALPLSGWDVELYGEWILAANDFSLESRWGARWSP
                     WKHILIGAEAAFPGNVLWYRLSVAGGSRSPYFWWRLSEDGDSMVGFGYRLDERISLEL
                     HYDERSEDTLTLKAISDL"
     gene            573..>1032
                     /locus_tag="PK849_15930"
     CDS             573..>1032
                     /locus_tag="PK849_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67674.1"
                     /translation="MEGVWTLRSLAERVEGRVIGDAALLVEGVASPSKARRGEIVCLW
                     NAFDTASGQKTDSPEPSPEAVNSSEGVLFLAAPEFFARHTDASGVAVEHPKKVFPLVL
                     DLFSRALRSGAQCGIHPTAVVSPKASIAPSAWIGPLCIIDEGAVIEERAFL"
BASE COUNT          199 a          268 c          323 g          242 t
ORIGIN      
        1 tcgaagcgct cgccgcagag gcgctgcttg agagcaatac cgtgtcgaac gcccgggcga
       61 cggtcgacgt ttctttctcc cccgagcaac tttctccggt gcaagcagaa gtagagagcc
      121 cgcgatattc tatccgggcg tggatagcag cgtatgccgg agcggacggg aagtaccctg
      181 agatcggcct tcacctggga agaaacgcgc tgccgctctc cgggtgggat gtagagctct
      241 acggcgaatg gatactcgca gccaacgatt tttcgttgga aagcagatgg ggagcgcgct
      301 ggagtccgtg gaagcacatc ctcatcggag cggaagcggc ctttccggga aatgttctct
      361 ggtatcggct ctcggttgca ggcggttccc gttctccgta tttctggtgg cgtctgagcg
      421 aggatggcga ttcaatggtt ggtttcggat accgcctcga cgagaggatc tccctggagc
      481 ttcactatga tgaacgttcg gaagatacgc tgacgctgaa ggcgatcagc gatctgtaaa
      541 ccgagaaatt tgctttgaac ggaggacatg gaatggaagg agtatggact ctccggtctc
      601 tagcggagag ggtcgaaggc agggtgatcg gagatgcggc gcttcttgtg gaaggcgtgg
      661 cctctccttc gaaggctcgc aggggagaga tcgtctgtct gtggaatgca ttcgatacgg
      721 cttccggaca aaaaacggac tctccggagc cttcgcctga agcggtcaat tcctccgagg
      781 gcgttctttt cctggcagcg ccggagtttt ttgcccgcca caccgatgcg tccggagtag
      841 ccgttgagca tccgaagaaa gtattccctc ttgtgcttga tcttttttct cgtgcgcttc
      901 gttccggcgc tcagtgcgga atccatccga cggcggttgt gtctccaaag gcgagtattg
      961 ctccatccgc ttggatcggt cctctctgta taattgacga aggcgccgtc atcgaggaac
     1021 gcgctttttt ac
//
LOCUS       DAOPKU010000803         1031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_178529_length_1031_cov_3.854508, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000803 DAOPKU010000000
VERSION     DAOPKU010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178529_length_1031_cov_3.854508"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK849_15935"
     CDS             <1..430
                     /locus_tag="PK849_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HPE67675.1"
                     /translation="TNDAPSASIRLIDMGIAPFLVASSLLAVIAQRLVRTLCPSCKEE
                     YIIPEPVCKTLGLPIDTRGYRPVGCNDCRGTGYKGRSAIFEIMLLNESIRELIVSGAS
                     ADVIRKEAIRLGMTTLRESGVRKIAAGVSSVEDVLTTTFS"
     gene            450..>1031
                     /locus_tag="PK849_15940"
     CDS             450..>1031
                     /locus_tag="PK849_15940"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPE67676.1"
                     /translation="MRIDELLAEVSVRGASDLHISEGLPPSFRVDGELRPLADFPSLS
                     REDMEEALQHLLSPEEMEKFRKSREHDFSFSFRSPDGEDARFRGNAYFESGRMGLALR
                     LIPMRIRTLDELGLPPTLKEISTHRRGLFLVTGPTGHGKSSTLATLLQMINTTRREHI
                     ITIEDPVEYVYISDKCIVHQRQVGIDTESFAEAL"
BASE COUNT          232 a          339 c          280 g          180 t
ORIGIN      
        1 caccaacgac gctccaagcg cgtcgatccg tctcatagac atggggatcg cccccttcct
       61 cgtcgcctcc tctctgctcg ccgtcatcgc tcagcgtctt gtgcgcacac tctgcccctc
      121 gtgcaaagag gaatacatta ttccagagcc ggtctgcaaa acactcggcc tccccatcga
      181 tacacgcggg taccgtcccg tggggtgcaa cgactgccgc ggaacgggct acaaagggcg
      241 gagcgcgatt ttcgagatta tgctgctcaa tgaatcgatc cgggaactca ttgtcagcgg
      301 cgcaagcgcc gacgtcatac ggaaggaagc gatacgcctg ggcatgacaa cgctgagaga
      361 gtcgggagta cggaagatag ccgccggtgt ttcgagtgtc gaagacgtgt tgaccacgac
      421 gttctcatga cggaaggaga ggatacctcg tgcggataga cgaactgctg gccgaagttt
      481 cggtacgcgg agcaagcgac cttcacatca gcgaaggact tccgccaagc ttccgcgtag
      541 acggagagct tcgtcccctc gccgatttcc cctcgctctc cagggaagac atggaggaag
      601 cgctgcaaca cctgctttcc cccgaggaga tggagaaatt ccgaaagagc cgcgagcacg
      661 acttcagctt ctcattccgg tctccggacg gagaagacgc ccgtttccgc ggcaacgcct
      721 acttcgaatc cggcagaatg gggctggcgc tccgcctcat cccgatgcgc atccgcaccc
      781 tcgacgaact cggactgccg ccgacgctca aagaaatttc cacgcaccgc agagggctct
      841 tcctggtaac cggccccacc ggccacggaa aaagctccac gctcgccacg ctcctccaga
      901 tgatcaatac cacccgcagg gagcacatca tcacaatcga agaccccgtc gagtacgtgt
      961 acatctccga caagtgcatc gtccaccaac gacaggtggg tatcgacacc gagagcttcg
     1021 ccgaagccct c
//
LOCUS       DAOPKU010000804         1016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_182764_length_1016_cov_4.530697, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000804 DAOPKU010000000
VERSION     DAOPKU010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182764_length_1016_cov_4.530697"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PK849_15945"
     CDS             <1..247
                     /locus_tag="PK849_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPE67677.1"
                     /translation="EHSYLKDTGRAKYVSVTDVEALDAFSRLCRKEGIIPAVESSHAL
                     AYALKRLPSMRKDQILLVNLSGRGDKDLETVLPMLGL"
     gene            510..>1016
                     /locus_tag="PK849_15950"
     CDS             510..>1016
                     /locus_tag="PK849_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE67678.1"
                     /translation="MIRKGCFVFFTLLWGLWCVAPLPALAGVHAVDDVEYSTPHGWVQ
                     QESPGAKAYHVLMYQGHPVGELFLMKESLFEQKSPATVLQEGIRKNAAGFAGYQAIGM
                     RNISLSGTEAVMHDFLYYPPGSPVQFAGRVVVLVVDKSAYTFFFNTTINTAAMLEGPF
                     SEVVSSVRV"
BASE COUNT          213 a          306 c          301 g          196 t
ORIGIN      
        1 cgagcactcc tacctgaagg acacgggccg ggcgaagtac gtttccgtca ccgacgtcga
       61 ggcgctggac gcattcagcc gtctctgcag gaaggaaggg atcatccccg cggtggaaag
      121 ctcgcacgcg ctggcgtacg cgctgaaacg cctcccctcg atgcgaaagg atcagatact
      181 gctggtcaat ctctccggac gcggagacaa agatctggag accgtgctgc cgatgctcgg
      241 gctgtagaca tgcgaacgct gccggagagc ccctccggca gcgctcactt ctctcaaaaa
      301 actacgctct tcccgccaaa gacaacctga tgaaaaagtg cgcatcgtgg tacaataatg
      361 cgacgatgca ggcccgatat gcgcaacgcc gcactttgag ctttcagaat cctccctcct
      421 gtgaaacgcg ctcggagggg gcgtcgttcc actctcacga gtacggcttc aggcgcagcg
      481 gaccgtcgat acgggaaggg ggaataccgg tgattcgcaa agggtgtttc gtattcttca
      541 cgttactctg gggtctgtgg tgcgttgcgc cgcttccggc gctcgcgggg gtgcatgccg
      601 ttgatgacgt tgaatactcc acgccgcacg gatgggtgca acaggaatct ccgggagcaa
      661 aggcgtacca tgtcctgatg taccaggggc atccggtcgg cgagctcttc ctgatgaaag
      721 agtccctctt cgaacagaaa agccccgcga cagtcctcca ggaaggcatc cggaagaacg
      781 ccgcaggatt tgcggggtac caggcgatcg gcatgagaaa catctcgctc tccggaaccg
      841 aggctgtgat gcacgacttc ctctactacc cgccggggag ccccgtgcag ttcgcgggaa
      901 gggtcgtcgt gctcgttgtg gacaaatccg cctacacctt tttcttcaat acgacgatca
      961 ataccgccgc catgctcgaa ggaccgttca gcgaagtcgt ctcctccgtc cgggtt
//
LOCUS       DAOPKU010000805         1011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_184192_length_1011_cov_3.933054, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000805 DAOPKU010000000
VERSION     DAOPKU010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184192_length_1011_cov_3.933054"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PK849_15955"
     CDS             <1..326
                     /locus_tag="PK849_15955"
                     /inference="COORDINATES: protein motif:HMM:NF013399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HPE67679.1"
                     /translation="AAMRSAAKVNHDVQTGGTLLNLRLNKEIVSTPRGLRNLGAMIRA
                     YFSLGAFHVQFNTISTGELRAAQARPEEYRDLLVRVAGYSTQFVNLSREMQEAIIART
                     EHASF"
     gene            313..>1011
                     /locus_tag="PK849_15960"
     CDS             313..>1011
                     /locus_tag="PK849_15960"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE67680.1"
                     /translation="MPPSEPFPKGEKICVSQIQHFSVNDGEGIRSTVFLSGCPLRCRW
                     CCNPETWTSTPKSGRLPGAKEETFGRFLSVAEVMKELVRHAIFYRESGGGVTWSGGEP
                     FFFPGNLRALVRACADRGIPQAAETTCHFDWDECADIVGMLDFVFADIKHMDGGVHER
                     LTGIGNERILSNMKRLGATGVDAVVRVPLLSGVNDDEQNVARTARFVYECFRQPKMEL
                     LPYHDFGRAKYRMLG"
BASE COUNT          181 a          317 c          337 g          176 t
ORIGIN      
        1 tggcggccat gcgttcggcg gccaaggtga atcacgacgt ccagaccggc gggacgctgc
       61 tcaacctgcg gctgaacaag gagatcgtct ccacgccgag aggcctgcgc aaccttgggg
      121 cgatgatacg cgcctacttc tccctgggcg ccttccatgt ccagttcaac accatctcca
      181 ccggggagct gcgcgcggcg caggcgcgcc cggaggagta ccgggacctt ctggtgcgcg
      241 tggcggggta cagcacgcag ttcgtgaacc tctcccgcga aatgcaggag gcgatcatcg
      301 ccaggacgga acatgcctcc ttctgagcct ttcccgaaag gggagaaaat ctgcgtctcg
      361 cagattcagc acttctccgt gaacgacgga gagggcatcc ggagcacggt cttcctctcc
      421 ggatgccctc tccgctgccg ctggtgctgc aacccggaga catggacgtc gacgccgaag
      481 agcgggcgtc ttccgggggc gaaggaggag accttcgggc gattcctcag cgtcgccgag
      541 gtgatgaagg agcttgtacg ccacgcgatc ttctaccggg agtcgggcgg cggggtcacc
      601 tggtcgggcg gggagccgtt cttctttccg gggaacctcc gggcgcttgt ccgggcctgc
      661 gcggacaggg gaatcccgca ggccgccgag acgacctgcc acttcgactg ggacgagtgc
      721 gccgacatcg tcgggatgct cgatttcgtc ttcgccgaca tcaagcacat ggacggcggc
      781 gttcacgagc gcctgaccgg aatcggcaac gagcgcatcc tctcgaatat gaagcggctt
      841 ggagcaaccg gagtcgacgc cgtcgtccgc gtgccgctgc tttccggcgt caacgacgac
      901 gagcagaacg tcgcgcgaac ggcgcgcttc gtgtacgaat gctttcggca gccgaaaatg
      961 gagctgcttc cgtatcacga ttttggacgt gcgaagtaca ggatgctcgg c
//
LOCUS       DAOPKU010000806         1010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_184647_length_1010_cov_2.321466, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000806 DAOPKU010000000
VERSION     DAOPKU010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184647_length_1010_cov_2.321466"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PK849_15965"
     CDS             <1..580
                     /locus_tag="PK849_15965"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE67681.1"
                     /translation="FVPGQVASATEINSREMMGAYGLNYMKDAGEVNVAADFVGYNEA
                     ADLLKNAQTDGTHIAGGLPTAAIMDALSSGVAEMISIEEDKIKEICEKYPWYFPFTIP
                     AGTYPNQEKEIKTLALANILFTDAKQDEELIYMLTKATYDYHEDLVNAHRATAYTVLE
                     NALNGMTVPLHPGSVKYFKEMGVDVPEKLIVK"
     gene            646..>1010
                     /locus_tag="PK849_15970"
     CDS             646..>1010
                     /locus_tag="PK849_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HPE67682.1"
                     /translation="MTQQNDLLEQLPELDAERAAEKVLEKADAEARFRKYGGRWAVAV
                     TVIAVAMSIFHLYTSGFGTLMAMKQRSVHLAFLVLLTFLLYPGSKKAPRNRPSVVDWI
                     WIILSVSSSLYIFFAFDAF"
BASE COUNT          238 a          280 c          277 g          215 t
ORIGIN      
        1 gttcgttccc gggcaggttg caagcgccac cgagatcaac agccgggaga tgatgggagc
       61 ctacggcttg aactatatga aggacgcggg agaagtgaac gtagccgccg acttcgtagg
      121 gtacaacgaa gccgccgacc ttctgaagaa cgcccagacc gacggaacgc atatcgccgg
      181 cggccttccc acagccgcga tcatggatgc gctctcctcc ggagtggccg aaatgatctc
      241 catcgaagag gacaagatca aagagatctg cgagaaatac ccctggtact tccccttcac
      301 aattcctgcg ggtacctatc cgaatcagga aaaggaaatc aagacgcttg ctcttgcgaa
      361 catccttttc acagacgcca agcaggatga agagcttatt tacatgctca ccaaggccac
      421 gtacgactat cacgaggatc ttgtgaacgc gcacagggcg accgcctaca cagtgctcga
      481 gaacgccctg aatggaatga ccgttccgct gcatccgggt tctgtgaagt actttaaaga
      541 aatgggcgtg gacgttcccg aaaagcttat agtgaaatag tgaccgattg aaagcgggga
      601 ggaggctccg ccttctcccc atctgtgtat tcggaggtag agagtgtgac acaacaaaac
      661 gatcttcttg agcaactccc tgaactcgac gcggagcgcg cggctgaaaa ggtactcgaa
      721 aaggccgacg ccgaagcgcg cttccggaag tacggcggcc gctgggccgt cgcggtgact
      781 gttatcgctg tagccatgtc catcttccat ttgtacacct ccggtttcgg gacgttgatg
      841 gcgatgaagc agcgcagcgt ccaccttgcc ttcctggtgc tcctcacatt tctgttgtat
      901 ccggggtcga aaaaagcgcc gcgcaaccgt ccgtccgtag tggactggat ctggattatt
      961 ctctcggttt catcgagtct ctatattttc ttcgcgttcg acgcctttag
//
LOCUS       DAOPKU010000807         1002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_186837_length_1002_cov_4.270327, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000807 DAOPKU010000000
VERSION     DAOPKU010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186837_length_1002_cov_4.270327"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PK849_15975"
     CDS             <1..979
                     /locus_tag="PK849_15975"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HPE67683.1"
                     /translation="PIRTLRNILRNPNFGGEIMVVGLGCEKLTADRLLEPEEIRPGNV
                     LMLQEHAGFVKMTDALMEMAERKLRRLEGRRRTTLPLSRLCVGMQCGGSDAFSGITAN
                     PAAGYASDLLVGAGATVLFSEVTEVRDGVKQLAARCVNSAVGRRLVEEMRWYDEYLRA
                     GGVDRSANPTPGNKKGGLANIVEKAMGSIAKSGRAPISEALAPGERPSAGGLVFAATP
                     ASDMVCGTCQLASGMTLQVFMTGRGTPYGLAAAPVIKVSSRSDLKDMWSDLIDVDAGV
                     IASGGADIRTVGERLFREIVEVASGRKRPAAERYRLFNDICLFNPARIT"
BASE COUNT          173 a          312 c          364 g          153 t
ORIGIN      
        1 tcccatcagg acgctgcgga acatactgcg gaacccgaac ttcggagggg agatcatggt
       61 cgtcggactg gggtgcgaga agctcaccgc ggacaggctg ctcgaaccgg aggagatccg
      121 ccccgggaat gtgctgatgc tccaggagca cgccgggttc gtcaagatga ccgacgccct
      181 gatggagatg gcggagcgga agctccgcag gctggagggc aggcgcagga cgacgctgcc
      241 cctctcgcgc ctctgcgtcg ggatgcagtg cggagggagc gacgcctttt ccggcattac
      301 cgcgaacccg gcggccgggt acgcgtcgga ccttctggtc ggggcgggcg cgaccgtgct
      361 cttttccgag gtcaccgaag tgcgggacgg cgttaagcag ctcgctgcgc gctgcgtgaa
      421 ctccgccgtg gggaggcggc tcgtcgaaga gatgcgatgg tacgacgagt acctccgtgc
      481 ggggggcgtg gaccgcagcg ccaaccccac tcccggaaac aagaagggcg ggctggcgaa
      541 catcgtcgag aaggcgatgg gctcgatcgc caagtcggga cgcgcgccga tttcggaggc
      601 gctcgccccc ggagagcgcc cgtccgccgg gggactcgtc ttcgccgcca cgcccgcgag
      661 cgacatggtc tgcggaacct gccagctcgc ctcgggcatg acgctccagg tgttcatgac
      721 ggggaggggg acgccctacg gactggccgc cgcgcctgtg atcaaggtct cctcgcggtc
      781 ggacctgaag gacatgtgga gtgacctgat cgacgtggac gcgggagtaa tcgcgtccgg
      841 cggggcggat atccggaccg tgggcgaacg gctcttccgc gagatcgtcg aggtcgcaag
      901 cgggcgaaag cgccctgccg ccgaacgcta ccggctgttc aacgatatct gccttttcaa
      961 cccggcccgg atcacgtaga atgctccatc aggagatttc cg
//
LOCUS       DAOPKU010000808         1001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3243
            NODE_187189_length_1001_cov_2.836152, whole genome shotgun
            sequence.
ACCESSION   DAOPKU010000808 DAOPKU010000000
VERSION     DAOPKU010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864978
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:24:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,178
            Genes (coding)                    :: 3,156
            CDSs (with protein)               :: 3,156
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 7 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187189_length_1001_cov_2.836152"
                     /isolate="AMR_MDS_3243"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PK849_15980"
     CDS             complement(<1..216)
                     /locus_tag="PK849_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPE67684.1"
                     /translation="MHEGAAIYDQAGELRRIVQTRRKNIKGLSGLRSIAILSGKGGVG
                     KSNVAVNLAMALAASGKRVVLLDADLGM"
     gene            complement(209..>1001)
                     /locus_tag="PK849_15985"
     CDS             complement(209..>1001)
                     /locus_tag="PK849_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HPE67685.1"
                     /translation="PVCGLGRKQDTDLLLRLTSEDVSEEHARKLVRDYESEAPSKDFS
                     EWLAGRIPVAADTNWDALGGGRVMLIGPTGVGKTTTIAKLAAIQSLWEKKNVVFLTAD
                     TYRIAAPEQLRTYAKILCVPIDVVYDANEIPDIVDRYRKADLLLLDTAGRSQKDSRRV
                     EEIRVLYEAFRPDAVHLVLPANMKYRDMLDVIQRMGVVPLSYVLFTKLDETTTYGALL
                     NILLDFDKPVSFFTTGQNVPNDIEVAKGSRLAELLLRGKGDVLRA"
BASE COUNT          209 a          308 c          274 g          210 t
ORIGIN      
        1 catcccgaga tccgcgtcga gaaggacgac gcgcttccct gaagcagcga gcgccatggc
       61 gagattcacc gcgacgttgc tctttcctac cccgcctttt ccactgagaa tggcgatgga
      121 acggaggccc gacagcccct tgatgttctt acggcgtgtt tgtacaattc tgcgaagctc
      181 tcccgcctga tcatagatcg ccgcaccctc atgcacgaag aacatctccc ttcccgcgaa
      241 ggaggagttc ggcgaggcgg gagcccttgg ccacttcaat atcgttcggg acgttctgcc
      301 ctgtggtgaa aaaagagacc ggtttgtcga agtcgagcag aatattcagc agggcgccgt
      361 aggtcgttgt ctcgtcaagc ttcgtgaaaa gaacgtacga aagcggaacg acgcccatcc
      421 gttgaatgac gtcgagcatg tcgcggtact tcatgttcgc gggaagcaca aggtggaccg
      481 catctggacg aaacgcctca tagagcacgc ggatttcttc gacgcggcga ctgtccttct
      541 gggaacggcc ggcggtatcc aacagcagaa ggtccgcctt ccggtaacga tccacgatgt
      601 ccggaatttc gttcgcgtcg tacactacat caatcggcac acagaggatc ttcgcatacg
      661 ttcgcagctg ctccggtgcg gcgatccggt atgtatccgc agtgaggaaa acaacgtttt
      721 tcttctccca gagcgactga atcgccgcaa gtttcgcgat cgtcgtggtc ttcccgactc
      781 cggtaggccc gatgagcatc acccggcctc cgccaagagc atcccagttt gtgtccgccg
      841 caacgggaat tctcccggcc agccactcgg agaagtcctt tgaaggagcc tcggattcgt
      901 agtcccgaac gagcttcctg gcgtgctctt cactcacatc ctccgaagtc agacgcaaca
      961 ggaggtctgt atcctgtttc ctccccagcc cgcagaccgg c
//
